LOCUS       DATKGS010000001         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_201081, whole genome shotgun sequence.
ACCESSION   DATKGS010000001 DATKGS010000000
VERSION     DATKGS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201081"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..378
                     /gene="rplX"
                     /locus_tag="VMQ48_00005"
     CDS             73..378
                     /gene="rplX"
                     /locus_tag="VMQ48_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HUD08470.1"
                     /translation="MKIKKGDNVKILSGKDRGKTGKVSRVLPDAGKALVEGLNLVKKH
                     IRPRKQGEKGQRVSVPAAIDISSLMLVCPKCSKATRVGFKVSEKDKFRVCKKCGSEI"
     gene            393..938
                     /gene="rplE"
                     /locus_tag="VMQ48_00010"
     CDS             393..938
                     /gene="rplE"
                     /locus_tag="VMQ48_00010"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HUD08471.1"
                     /translation="MASFLKEKYNKEVIAKLKEKFGAKNAMAVPKILKVSLNSGIGKY
                     LKEKDAIDEIAENLKKISGQKPVFAKSKKSVAGFKVREGQEVGVSVTLRGERMWHFLE
                     RLVSSALPRVRDFRGIDPKNVDRDGNLNMALKEQFVFPEIVPEGVKTIFSFQVNVTTS
                     AKTKEEGLELFKLLGFPIKQD"
     gene            947..1132
                     /locus_tag="VMQ48_00015"
     CDS             947..1132
                     /locus_tag="VMQ48_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HUD08472.1"
                     /translation="MAKTSTEARARKKPKFSSRIVRRCWRCGRKHGYMRDFELCRICF
                     RELASVGEIPGVKKSSW"
     gene            1150..1554
                     /gene="rpsH"
                     /locus_tag="VMQ48_00020"
     CDS             1150..1554
                     /gene="rpsH"
                     /locus_tag="VMQ48_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HUD08473.1"
                     /translation="MLDPISDMLTRIRNANLAGHTEVFVPLSKLKLGIAGILKKKNFV
                     EKIGEDEVEGRKRIRIDLKYIRDEKGKKSPYIKGLSRVSRQGQRIYAGKGELPVVRSG
                     YGFSIVSTSKGLMTGEEAKKTGVGGEIICEIW"
     gene            1621..>2064
                     /gene="rplF"
                     /locus_tag="VMQ48_00025"
     CDS             1621..>2064
                     /gene="rplF"
                     /locus_tag="VMQ48_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HUD08474.1"
                     /translation="MSRVGKKTISVPDAVGINISGQNISVKGPKGTLDFSFDWDVVVK
                     HEEKEIQVSVKKITKQTKALWGLTRVLISNMIEGTTKGFEKQLELQGVGYRMAVAGKK
                     INMALGFSHPVDVEIPEGLEAKIEKNILTVSGIDKQKVGQFALLFP"
BASE COUNT          684 a          349 c          552 g          479 t
ORIGIN      
        1 ggagcgccag aagtttggta gcaaaaacac gaatttaacg aataagagca cgaatacaac
       61 gaaaaaaaga ttatgaagat caagaagggt gacaatgtaa aaatactttc cggaaaggac
      121 cggggaaaga cgggaaaagt ttcacgcgtt ttgcctgatg ccggaaaagc gcttgttgaa
      181 ggattgaatc tggtgaaaaa gcacattcgt ccccgcaagc agggtgaaaa gggacaaaga
      241 gtttccgttc cggcggcgat tgatatttcg agtttgatgc tggtttgtcc gaagtgctcg
      301 aaagctaccc gggtcgggtt caaagtcagt gaaaaagaca aatttagggt atgcaagaag
      361 tgcggaagcg agatataaga aggaaaaaat atatggcgag ttttctcaag gaaaaatata
      421 ataaggaagt gattgcgaag ctgaaggaaa aattcggcgc gaagaatgct atggctgttc
      481 cgaagatttt gaaagtgtct ctcaacagcg gaatcggaaa atatctcaaa gaaaaagacg
      541 ccattgatga aatcgcggaa aacctgaaaa agatcagtgg tcaaaaaccc gtttttgcaa
      601 aatcaaagaa atcagttgcc ggattcaagg tccgggaagg tcaagaggtc ggagtttcgg
      661 tgacgctccg cggcgagaga atgtggcatt ttttggagag acttgtttcg tcagctctgc
      721 cgcgcgtgag agattttcgt ggaattgatc cgaaaaatgt tgatcgggac ggaaatttga
      781 acatggccct caaagaacag tttgtttttc ccgaaattgt gccggaaggt gtgaaaacca
      841 ttttcagttt ccaagtgaat gtgacgacga gcgccaaaac gaaggaagaa ggattagaat
      901 tatttaaatt gctaggattt ccgataaaac aagattaata aatattatgg ccaagacatc
      961 aactgaggca agagccagga aaaaaccgaa attttcttct cgcattgtgc gccgctgttg
     1021 gcgctgtgga agaaagcacg gatatatgcg ggattttgaa ttgtgccgaa tttgtttccg
     1081 cgagcttgcc agcgttgggg agattccggg agtcaagaag tcaagttggt aaattagtat
     1141 cacacaaata tgttggatcc aattagcgat atgctcacaa gaataaggaa cgcaaacctt
     1201 gccggccaca cggaggtttt tgtgccgctt tcaaagttga aactcggtat cgccggaatt
     1261 ttgaaaaaga aaaattttgt tgaaaaaatc ggtgaagatg aagttgaagg ccgcaaacgc
     1321 attcgtattg atctcaaata catccgggat gaaaaaggca aaaaatcacc atacatcaaa
     1381 ggacttagcc gggtgagccg acagggacag agaatatatg cgggcaaagg agaattgccg
     1441 gtcgtgagga gcgggtacgg attttccata gtctcaactt cgaagggctt gatgacgggt
     1501 gaagaggcca aaaagacagg cgtgggggga gaaattattt gtgagatctg gtaatagcaa
     1561 catacactaa cgcaacgaat gagggcacga atacaacgaa aattagttta atttaatatc
     1621 atgagcagag taggcaaaaa aacgatcagt gttccggatg cagtgggtat caatatttcc
     1681 ggacaaaata tatccgtcaa aggaccgaaa gggacgcttg atttttcttt tgattgggat
     1741 gttgtggtga agcatgaaga aaaagagatc caggtgtccg tgaaaaaaat caccaagcag
     1801 acaaaagcgc tttggggttt gacgcgggtt ttgatttcca atatgatcga agggacaacc
     1861 aaaggttttg aaaaacagct ggaacttcaa ggtgttggat acagaatggc ggttgccggg
     1921 aaaaaaataa atatggcgct cggattttct catccggtcg atgtggaaat tccggaagga
     1981 cttgaggcga aaattgaaaa aaatattctg acggtttctg gaatcgacaa gcagaaagtt
     2041 ggacaatttg ccctcctctt cccc
//
LOCUS       DATKGS010000002         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_71862, whole genome shotgun sequence.
ACCESSION   DATKGS010000002 DATKGS010000000
VERSION     DATKGS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71862"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..418)
                     /locus_tag="VMQ48_00030"
     CDS             complement(56..418)
                     /locus_tag="VMQ48_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08475.1"
                     /translation="MSQFAAISHSNSVVFGERKKAVKTKPEKASRITWSFLLVSLICA
                     AGVFYIFEVNNAATQGYRIQALEKQAQELRDSNEKLKIKEAELRSMYNIEEKTKDLNM
                     TAPKDVSYLTLPGNVAMK"
     gene            complement(457..1512)
                     /gene="rsmH"
                     /locus_tag="VMQ48_00035"
     CDS             complement(457..1512)
                     /gene="rsmH"
                     /locus_tag="VMQ48_00035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HUD08476.1"
                     /translation="MIHKSVLLEELVENLKLKPGMTVVDGTLGAGGHSEEILKRILPG
                     GKLISIDWDKRAAENFKKKLEKGISIAKKELVRLAASVSVNINKKNDDQVSSQFEKLG
                     NRGGEIVGYWHGAADNYANIKEILGGLGIRSVDAVFVDLGFSSDQIEDEKRGFSFLKD
                     GPLDMRYAPDSQKRSASDIVKFYTEEKLTEIFKIFGEEKFAKRISREIVLARRSSDIR
                     KTTELVKIISAAVPEKYKRGKIHFATRVFQALRIETNQELENLKNFLNQAVEALASKG
                     ILATISFHSLEDRIVKNFFREEAQDCVCPPNFPKCVCAHRARLKIVTKKPITASEREV
                     AQNPRARSAKLRIAQKI"
     gene            complement(1516..1950)
                     /gene="mraZ"
                     /locus_tag="VMQ48_00040"
     CDS             complement(1516..1950)
                     /gene="mraZ"
                     /locus_tag="VMQ48_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HUD08477.1"
                     /translation="MFIGEYNHNIDPKKRVAIPAKFRNDFSGKAVVTRGLDKCLFIYP
                     LKTWEELSSKLGSLPMGESGTRSFVRLMLAGAVDVDIDKLGRVLIPDYLKEYAGLQKE
                     VIVAGLYNRLEIWDSGKWSQYKKDAEKNSDEIAEQLGKLGIY"
     gene            complement(2118..>2428)
                     /locus_tag="VMQ48_00045"
     CDS             complement(2118..>2428)
                     /locus_tag="VMQ48_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08478.1"
                     /translation="SSILALTRNIAGALGIAVFGTILNNSTSSKVLEIARYSSLHAYT
                     PNFVGQFTSLIILKAQIEAYRTVFLYAFVILVVGAILSLTIKVKEEIKPGGEEVLILD
                     "
BASE COUNT          569 a          629 c          466 g          764 t
ORIGIN      
        1 gggagcgtat gtttttttgt gcttagatat tttcatggca atgatcagtg aaaaatcatt
       61 tcatcgccac gtttcccggc aatgtcagat aactcacatc tttcggcgcg gtcatgttga
      121 gatcttttgt tttttcttca atgttgtaca tcgaacgcag ctctgcttcc ttgattttca
      181 gtttctcgtt gctgtctctc aattcttgcg cttgtttttc aagcgcctgg atcctgtatc
      241 cttgcgtcgc ggcgttgttc acttcgaaga tatagaatac cccggccgca caaatcaaag
      301 acaccagcaa gaatgaccag gttatgcggc tggctttctc cggtttggtt ttcacagcct
      361 tttttctttc tccaaaaaca actgaattgg agtggctgat tgcagcgaat tggctcattg
      421 gactttcgtc cccctgtttt actttttttg tttttgttat attttttgcg ctatcctcaa
      481 cttcgcactt cttgctcgcg gattttgagc aacttctctc tctgaggcag tgatcggctt
      541 tttcgtgacg attttgagcc gcgctctatg agcgcaaaca catttcggaa aattgggcgg
      601 gcaaacgcaa tcttgcgctt cttcgcgaaa aaaattcttc acgatccgat cttcaagcga
      661 atgaaagctg atagtggcta atatcccctt actagccagc gcctcaactg cctggttcaa
      721 aaaatttttc aaattttcaa gttcctgatt ggtttcgatc ctgagcgcct gaaatactcg
      781 cgtcgcaaaa tgtatttttc ctcgcttgta tttttccggt actgctgcag atataatttt
      841 cacaagttct gtcgtctttc ttatgtcgct gcttctcctc gccagcacaa tttctcggga
      901 tattctcttg gcaaatttct cttctccaaa tattttgaat atttcagtca acttttcctc
      961 ggtataaaac ttcacaatat ctgacgccga ccgtttttgg gagtccggcg catagcgcat
     1021 gtcgagcgga ccatccttga gaaagctgaa tcccctcttc tcgtcttcga tctggtcgga
     1081 gctgaatccg aggtcgacga aaactgcgtc tacggatcga atccccagcc ccccgagaat
     1141 ttcttttata ttggcataat tgtcagccgc tccgtgccag tacccgacga tctcgccccc
     1201 gcgattcccc agtttctcaa attgagaaga gacttgatcg tcattttttt tattaatatt
     1261 gaccgacacg gaagccgcca aacgaaccaa ttcttttttt gcaatagaaa ttcctttctc
     1321 gagtttcttt ttaaaattct cagccgctct tttatcccaa tcaatcgaaa tcaatttccc
     1381 gcccggcaat attcttttca gtatttcttc tgaatgtcct cctgccccaa gcgttccatc
     1441 cacaactgtc attcccggct tcaatttcag attttcgaca agctcctcca gcaagactga
     1501 tttgtgaatc atatcttagt aaattcccaa tttacccaac tgttctgcga tctcgtctga
     1561 atttttttcc gcgtctttct tgtactggct ccacttgccg ctgtcccaga tttccagacg
     1621 gttatagagg cccgcgacta tcacttcttt ttggagtccg gcatattctt tgagatagtc
     1681 agggataagc actcgtccca atttgtcgat atccacatcg accgctccgg cgagcatcag
     1741 tctcacaaaa cttcttgttc ctgattcccc catcggcaga cttccgagct tggatgacaa
     1801 ctcttcccaa gtcttgagcg gatatatgaa aagacatttg tccaaacccc gggtgacaac
     1861 cgctttcccg ctgaaatcat ttctgaactt cgccggaatg gccacgcgtt ttttgggatc
     1921 tatattatga ttgtattcgc cgatgaacat aagttacgca cagattgacc tagttatccc
     1981 cacttccatc cacttcgcaa cactgtacat acattctaat ccactctgaa acatccgtca
     2041 acactttgca ggcaaaataa aaatggctta tacaagccac tggaaagcat tttccccaca
     2101 agttatccac tttttttcta atccaaaatc aaaacctctt ctccccctgg tttgatttct
     2161 tctttcactt tgatagtcaa agacaaaatc gcgccgacga ccaagattac aaaggcgtac
     2221 aaaaatacgg tgcgataggc ttcgatttgc gctttgagga tgatgagcga cgtgaactgg
     2281 ccgacaaaat tcggggtata agcatggagc gaactgtagc gcgcgatttc cagcactttc
     2341 gagctagtag aattattgag tatcgttcca aaaaccgcga tcccaagcgc tccggcgatg
     2401 tttcgggtga gcgccagaat ggacgagg
//
LOCUS       DATKGS010000003         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_172474, whole genome shotgun sequence.
ACCESSION   DATKGS010000003 DATKGS010000000
VERSION     DATKGS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172474"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1527)
                     /locus_tag="VMQ48_00050"
     CDS             complement(196..1527)
                     /locus_tag="VMQ48_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIF1 family DEAD/DEAH box helicase"
                     /protein_id="HUD08479.1"
                     /translation="MKQRTALDILKLGHNVFLTGPAGSGKTFLLNQYIAHLKSKKIGV
                     AVTASTGIAATHMNGQTIHSWSGMGIKDELTEGDLNKLKRKFFYRKRFAKTHTLIVDE
                     ISMLHAHQLDMVNKICKAFKDPFLPFGGLQVILCGDFFQLPPVSKNSQKAKFAMESLA
                     WQEMNPKICYLEEQYRQEDEKMSKILNAIRNQKVDDQILSLILSRENQPTRSDIYPTR
                     IFTHNANADAINNLELQRIKEKPLAYTMQSDGNDKLVSVIKKGCLAPERLVLKKGAVV
                     MFVKNNFDRGFVNGTLGKIVDFDENKLPIVKTFLGAEIVALPEKWAIEEDDRVLASVT
                     QIPLRLAWAITVHKSQGMTLDAAEMDLSKSFEYGMGYVALSRLRTLDGIKLLGINETA
                     LKVNPEICELDKELEKMSDMNFQEIAGMSRKNKKKLQEDFIEKNSGKRRRK"
     gene            complement(1648..2706)
                     /locus_tag="VMQ48_00055"
     CDS             complement(1648..2706)
                     /locus_tag="VMQ48_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HUD08480.1"
                     /translation="MKKNTKQIVANNSFTEFLLYTTPNGKVKVEIFLRDENIWLTQKR
                     IAELFDVGIPAVNKHLTNIFESGELNENSVISILEITADDGKNYKTKFYNLDAIISVG
                     YRVNSAQATQFRIWATERLKEYIIKGFTMDDERLKDPNGFFGKDYFEEQLARIRNIRS
                     SERRMYQKVTDIYAQCSADYNPNEEITKQFFATVQNKLHFAITGKTAAEIIHERVNSA
                     KKNMGLTVWKNSPKGAIRETDVVIAKNYLNEKELDHLNRIVTMYLDYAEMQATRGILM
                     RMKDWVEKLDAFLKFNERQILGNAGKISHEVAEALALGEYEKFRIEQDRNYVSDFDRE
                     VEKILTMAKKKKFSKKKK"
     gene            complement(2819..4030)
                     /locus_tag="VMQ48_00060"
                     /pseudo
     CDS             complement(2819..4030)
                     /locus_tag="VMQ48_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(4013..4087)
                     /locus_tag="VMQ48_00065"
                     /pseudo
     CDS             complement(4013..4087)
                     /locus_tag="VMQ48_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          958 a         1039 c          793 g         1332 t
ORIGIN      
        1 taaaagatcg cttttagaaa gagcatcggg gcctttttga agaaattttt ctctaggtcg
       61 atctattttc ggaatatctt ttatttttgc catatatctt tcggacacaa aattttcttc
      121 tttaaataaa gaatatttat acccccatat tatcattgat tcgccgaaaa gtaaaggatt
      181 ttgcccatat ttacgctatt ttctccgtcg cttgccgcta ttcttttcga tgaaatcttc
      241 ttgcagtttt ttcttgttct ttcggctcat tccggcaatc tcttggaaat tcatatccga
      301 cattttttcc aattccttgt ccagttcgca aatttccgga ttgactttga gagccgtttc
      361 gttgattccc aaaagcttta tgccgtccaa agttctgaga cgtgagagcg ccacatatcc
      421 cattccatat tcgaaagatt tgctcaaatc catttctgcc gcatcgagag tcataccctg
      481 gcttttgtgc acggtgatcg cccaggccag gcgaagcggg atctgggtga cggaggcgag
      541 aacccggtcg tcttcttcaa tcgcccactt ttccggaaga gccacaattt ctgctcccaa
      601 aaatgttttg acaatgggca gtttgttttc gtcaaagtcc acaatttttc ccagtgtgcc
      661 attcacgaag ccgcggtcga aattgttttt gacaaacatc accaccgctc cctttttcag
      721 caccagccgt tccggagcaa gacatccttt tttgatgacg ctgaccaatt tgtcattgcc
      781 gtccgattgc attgtatagg ccagcggttt ttctttgatc ctctggagtt ccagattgtt
      841 gatcgcatcc gcgttggcat tgtgggtgaa gatcctcgtc ggatatatat cgcttcttgt
      901 cggctggttt tctctggaca atatcaagct caagatctga tcatccactt tctggtttcg
      961 gatagcgttg aggattttcg acatcttctc gtcttcttgg cggtattgtt cttccagata
     1021 gcaaatcttt ggattcattt cctgccaagc gagagactcc atcgcgaatt ttgccttctg
     1081 gctgtttttg ctcaccgggg gaagttgaaa aaagtcaccg caaagaatca cctgcagtcc
     1141 gccaaagggc agaaaggggt ctttgaaggc cttgcagatc ttgttgacca tatccagctg
     1201 atgggcatga agcatggata tctcgtcaac aatcaaagta tgggttttgg caaaacgttt
     1261 gcgataaaag aattttctct tgagtttgtt cagatctccc tcagtcagtt cgtctttgat
     1321 gcccatcccc gaccagctgt gaatcgtttg cccgttcatg tgagtggcgg caataccggt
     1381 cgaagccgtg acggccaccc cgattttttt gctcttgaga tgcgcgatgt attggttcaa
     1441 caaaaaagtt ttcccgcttc cggcggggcc tgtcaggaac acattgtgtc ccagtttcaa
     1501 aatgtccaga gctgttcgct gtttcacaga attttgcctt gtttatgctc taattctagc
     1561 aaaaaaccca attattttgg gtatcttttg taaggtttgc aagtttttgc ggttgtcctg
     1621 ataccaaaag agtcaaaacc agtcggatca ttttttcttt tttgaaaatt tctttttctt
     1681 tgccatcgtc aaaatcttct ccacctcacg atcaaaatcc gaaacataat tccgatcctg
     1741 ctcgattctg aatttttcat attctcccag cgccaaagct tccgccactt catggctaat
     1801 tttcccggca ttgccaagta tttgccgttc attgaatttc aaaaacgcat ccagcttttc
     1861 cacccaatct ttcatccgca tcagaatccc gcgagtcgct tgcatttcag catagtcaag
     1921 atacatcgtc acaattctat tgagatggtc caattctttt tcgttcaaat agtttttcgc
     1981 aatcaccaca tcagtttccc gaatcgcacc ttttggagaa tttttccaaa cggtcagtcc
     2041 catatttttc ttggcgctat tgaccctttc gtgaattatc tctgccgcag ttttgccggt
     2101 aatggcaaaa tgcagcttat tttgcacagt ggcaaaaaat tgcttggtga tttcttcgtt
     2161 gggattataa tcagcgctac actgagcata aatatcagtc actttttgat acattctcct
     2221 ttcactagaa cgtatatttc tgattctcgc cagctgttct tcaaaataat ctttgccaaa
     2281 aaacccatta ggatccttga gtctttcgtc atccatcgta aaacctttga taatatactc
     2341 ttttaacctt tcagttgccc aaatgcgaaa ctgcgtcgct tgcgcgctat tcacgcgata
     2401 accaacagaa ataatggcat ctagattata aaattttgtt ttgtagtttt tgccgtcatc
     2461 ggcagttatt tccaaaatgg aaataactga attttcattc aactccccgc tttcaaagat
     2521 gtttgttaaa tgtttattga cagcgggaat accaacatcg aaaagctcgg ctattctttt
     2581 ttgcgtcaac caaatatttt catcgcgcaa aaatatctcc acctttactt ttccgtttgg
     2641 cgtagtgtag agcaaaaact ccgtaaaact attatttgca acgatttgct tagtattttt
     2701 tttcatatat ttgttacgta gcatccatca taggcggaca agtgctgcat aacagtattt
     2761 ttcctaacat tcaatgttag gaacggctac tatcactata tcactatatt aacctctgtc
     2821 aagccgcccc tgtccccgcc gcgtatccgg tgctccagca gatctgcaga ttgtatccgc
     2881 cggtcgggcc gtccacgtcc aatatttcgc cggcgaaata gagattgccg atcaatcggg
     2941 attgcatggt tttggaattc acttctttca agctcactcc cccgctggtg acgaccgctt
     3001 gagcgtatcc ggccgtgccg tcgaatgtca ggcgcagatc tttgagaaga agcgccaatt
     3061 ttttccgctc cgctcccgtg accgtgttga tttttttgcg cgggtcaatg ccggacaatt
     3121 tgatcattgt cgcgatcatt ttttgcggca aaagttccgg caagtagttc acgaaatctt
     3181 ttttcgcgtt gtccgcgaaa tctctttgca gccgagtgtc gagttgttcg gggctcaagg
     3241 ccggttttag gtcgatcgag accgcgactt cgcctttcgc catcaattct ccggccttct
     3301 tgctgatgtc gagcacaatc ggaccgctca agccgaaatg cgtgaaaagc atttccccaa
     3361 atcttgaatc tttctttttg ttgttctgga tcaaacttat ctccacattt ttcaaactca
     3421 agccctgcac ctctttgacc caagtctctt tggttttgat cggtaccaaa gccggcgcgg
     3481 gcgggacaat cgtatgcccc aagtctttcg cccattgata tccgtctccg gtcgagccgg
     3541 tgagcggata ggattttccg cccgtgcaca gaataaatat gtccgctgaa attatttttc
     3601 tgcccggttt tcctttttca tgcttcaata ttacgccttt tatttttttc ccgtcacttt
     3661 caaatcaaac actttcgctg ccaaacaata ttttcacttt gttttttttc aaatattcct
     3721 ccaaaaccct caaaacgtcg tatgacttgt tgctcaccgg aaaaatacgc ccgcctcttt
     3781 cggttttgac aggcaacttt ctgtcctcaa aaaattttat cacttctctg ggtccaaaaa
     3841 ctgacagaga cgaaaacaaa aattttccat tcttgccgaa ctggctgacc atttctttga
     3901 gatcaaactc gtctcgcgtc agattgcagc ggcccttgcc cgtgatcaaa agttttttgc
     3961 caagactctc gtttttttcc aacagcacca ctcgcgcgcc catttccgcc gcccggcccg
     4021 ccgccatcat gcccgccggt ccaccgccaa tgaccgccac gtcaaatttt tcccgttggt
     4081 ttttcatgca agaatggtag catgtttcgc ccttccgtcc aa
//
LOCUS       DATKGS010000004         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_207, whole genome shotgun sequence.
ACCESSION   DATKGS010000004 DATKGS010000000
VERSION     DATKGS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VMQ48_00070"
     CDS             complement(<1..752)
                     /locus_tag="VMQ48_00070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HUD08481.1"
                     /translation="MNNTKKITRAMEMVAASKMRRAVASVLAIRSYAHSAWGVLTNMA
                     RAFENYPVDLLEAREVKKICIILVTSNRGLCGGFNAQILRKVIEQVKNPALLKINRVG
                     NKRIDSKIPDSEIEIDFITVGKKGAAVVRKLGKNVVATFDELTYLPGIVDVRALSRIV
                     IDDYSAKKYDKVVVAFTDYVSAVVQHPKLRQLLPVSKVDIEKQLADMDVLAKEYGLNE
                     PPMEYKVEPDAKTVLTEILPRLIEMQVYHAIL"
     gene            complement(806..2350)
                     /gene="atpA"
                     /locus_tag="VMQ48_00075"
     CDS             complement(806..2350)
                     /gene="atpA"
                     /locus_tag="VMQ48_00075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HUD08482.1"
                     /translation="MNQKDFIVESLKKQISGFKAETKSEKVGRVVEVGDGIARVSGLS
                     DAMASEMLEFESKGGQPVFGVALNLEEDQVGAMILGEYEKIKEGDTVRSTGKILSVPV
                     GEEMIGRVVSPLGRPIDGKGELSAQGGPASGRKNYPIEKIAAGVIERESVKQPLQTGI
                     KAIDSMIPIGRGQRELIIGDRQTGKTAVAIDAIINQKGEDVICIYVAIGQKESKVARI
                     VAELEKRGAMAYTIVVIAGASDPAALSFIAPYAGCAMGEYFMDQGKDVLVIYDDLSKH
                     AWAYRQVSLLLKRPPGREAYPGDIFYLHSRLLERSAKLSKEMGGGSITALPIIETQAG
                     DVSAYIPTNVISITDGQIYLESDLFYKGIRPALNVGLSVSRVGSSAQIKAMKKVAGKL
                     RLDLAQFRELEAFAQFGSDLDETTRNQIERGRRTVEILKQDQYVPMPVENQVAILFAL
                     VGGYLDDVPVENIQKWEAEFHAYMKDMKSDVLGLIKEKKELTEEVEKALKKAIEEYKE
                     VYGSDN"
BASE COUNT          547 a          697 c          562 g          699 t
ORIGIN      
        1 tccaaaatgg cgtgatagac ttgcatctca atcagccgcg gcagaatttc cgtgagaacc
       61 gttttcgcgt ccggttcgac tttgtattcc atcggcggct cattgaggcc atactctttg
      121 gccaaaacgt ccatgtccgc cagttgtttt tcgatatcca cttttgaaac cggaaggagc
      181 tggcgaagct tgggatgctg gaccacggcg gatacatagt cggtgaaagc aaccaccact
      241 ttgtcatatt ttttcgcgct ataatcgtca atgacaattc tcgagagtgc ccggacgtca
      301 acgatccccg gaagataggt cagctcatca aaagtggcca caacattttt cccgagtttg
      361 cggacgaccg ccgctccctt tttcccaacg gtgatgaaat caatttcaat ttcgctgtcg
      421 ggaattttcg aatctatccg tttgttcccg acccggttta ttttgagaag cgccggattt
      481 ttcacctgct caatcacttt cctcaaaatc tgggcattga acccgccgca aagtccccgg
      541 ttggaagtca ccaaaattat gcaaattttt ttcacctcgc gcgcttcaag caggtcaacc
      601 ggatagtttt caaacgcgcg ggccatgttg gtcagtactc cccaggcgct gtgcgcgtag
      661 gaacggattg ccaaaaccga cgccaccgcc cggcgcatct tcgaagccgc taccatttcc
      721 atcgcgcggg tgattttttt cgtattgttc accgatttta ttcggcgacg tatttctctg
      781 cttccttgtg ccattggctg aaatcttaat tatcgctccc gtatacctct ttgtactcct
      841 caatcgcctt tttgagcgct ttttccactt cttccgtcag ctcttttttc tcttttataa
      901 gccccaaaac gtcgctcttc atgtctttca tatatgcgtg gaattcggct tcccatttct
      961 ggatattttc aacgggaaca tcatcgagat atccgccgac gagcgcaaaa agaatcgcca
     1021 cttggttctc gaccggcatc ggaacatact ggtcctgctt caaaatttca accgtacgcc
     1081 gtccgcgctc gatctgattc cgggttgttt catcgagatc tgaaccgaac tgcgcgaaag
     1141 cttcaagctc gcggaactgg gcaaggtcaa gccgcaattt cccggccact tttttcatcg
     1201 ccttgatctg ggcggaagaa cccacgcgcg acactgaaag accgacgttc agtgccggac
     1261 ggatgccctt gtagaaaaga tcagattcga gatagatctg tccgtccgtg atggagatga
     1321 cgttggtcgg aatgtaggcg gacacatcgc cggcttgcgt ttcgatgatc ggaagcgccg
     1381 tgatcgatcc gccgcccatt tctttcgaca gtttcgcgct cctttcgagc agccgcgaat
     1441 gcagatagaa aatatctccc ggataagctt cgcgtcccgg cggacgtttg agaagaagcg
     1501 aaacttggcg ataggcccaa gcgtgcttcg aaagatcgtc atatatcacc aggacatctt
     1561 ttccttggtc catgaaatat tctcccatcg cgcagccggc gtacggtgcg atgaaagaaa
     1621 gagcggccgg atcggaagcg cccgcgatga cgacgatcgt ataggccatc gcgccccgct
     1681 tctcaagttc cgcgacaatt ctcgccactt tcgattcttt ctggccgatc gctacatata
     1741 tacagatgac atcctccccc ttttggttga tgatcgcgtc gattgccacg gcggtttttc
     1801 cggtttgacg gtcgccgatg atgagctcgc gctgtccccg gccaatcgga atcatcgaat
     1861 caatcgcttt gatcccggtc tggagcggct gcttcactga ttcgcgctca atcacacccg
     1921 ccgcgatttt ttcgatcgga taatttttcc ggccggaggc tggtccgcct tgggcggata
     1981 attccccttt cccatcaatt ggccttccta aaggactcac cactcgtcca atcatttctt
     2041 cgcccaccgg cacggacaaa atttttccgg tcgaacgtac cgtgtcgcct tctttgatct
     2101 tttcatattc gccgaggatc atcgctccga cctggtcctc ttcaaggttg agcgccacgc
     2161 cgaaaaccgg ctgtccgcct ttgctctcga actcgagcat ttcgctggcc atggcatccg
     2221 acagtcccga cacgcgggcg attccatcgc ccacttcaac aacccggccg actttctcgg
     2281 atttggtttc ggccttgaag ccggagattt gttttttgag agactctact atgaagtctt
     2341 tttggttcat aaattttctg aattaatttt agaattacaa aattttcaat tttcaaacaa
     2401 tgacttaatt tcaaaatttt gaaattgttt ttgaaattga aaattcagaa attttgaaat
     2461 tctatgttaa taattttttc aactctccca accgtccagc tatgc
//
LOCUS       DATKGS010000005         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_43283, whole genome shotgun sequence.
ACCESSION   DATKGS010000005 DATKGS010000000
VERSION     DATKGS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43283"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VMQ48_00080"
     CDS             complement(<1..530)
                     /locus_tag="VMQ48_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HUD08483.1"
                     /translation="MKNIYKNMHSCNLDVEISKLSKIEGNADLDIKIRDGKLQEVNLK
                     VMENLRFFNQAVKGQSYAAVPQLVSRICGTCSIAHLTGCIEAVEKTLGVKISEQTLIL
                     RKLTVYSMMIRDHALHLYFFAMPDMLGIDSLLDVSEDKHDLIHDALAVKEAGNNLSEL
                     IAGRAVHAPFEQVGGF"
     gene            complement(514..984)
                     /locus_tag="VMQ48_00085"
     CDS             complement(514..984)
                     /locus_tag="VMQ48_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08484.1"
                     /translation="MAETNNKKRVGIFSLTCDEGCSILLTEIFNQKLIPWLEKMELVY
                     FLSIKDKVEIKNLDIALVEGVVTSERDKEDIEKIRANSKILIAMGTCAMTALPSGQRN
                     NFNISQLKEIEGHLEKYNFLPKALALKDAVKVDDSISGCPIDEGKFIETFEKYL"
     gene            complement(987..2018)
                     /locus_tag="VMQ48_00090"
     CDS             complement(987..2018)
                     /locus_tag="VMQ48_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HUD08485.1"
                     /translation="MDNKLLNFIQQNSPDGGFLQSEYWRKFQESVGRKTYNLSENGED
                     GELTVSANIIAHRLPLISDYFYAPRGPVFSIFNFQFSIFKQFLNDLTDNAKENNIGWI
                     RIEPNSEEELELIQDNLPKDWRTKKSSVDMQPREILVLDISKSEEEILAGMKQKTRYN
                     IKLAEKRGVHVTYNMKHVTQENIDKFLQLVKITSKRDKITSHPDAYYRQMFETIPSEI
                     LKLYIAEYEGKIIAANLVLFFGKTATYMHGASDNEHRNLMAPYLLQWQQIEDAKKAGC
                     ERYDFGGIKTAISDQRSMNNSWSGITKFKTGFAPNTNPIQFPGCYDIILKPGKYNLYR
                     ALQKIKRIF"
     gene            complement(2029..2598)
                     /gene="ruvA"
                     /locus_tag="VMQ48_00095"
     CDS             complement(2029..2598)
                     /gene="ruvA"
                     /locus_tag="VMQ48_00095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HUD08486.1"
                     /translation="MIAKIKGKIAFLRDHYAVVDVNGVGYKIFVTDFTMGKIAGKEEI
                     ELFTHTYVREDSLSLYGFLTIDELGMFELLISISGIGPKAAIGILTIAEPKTIRTAII
                     TGDSSILTRVSGVGKKTAERVILELSNKIAELPGEDQGAARADSEAIEALASLGYSVS
                     QAREALKAVPESVKDVSERVRAALKSLGK"
BASE COUNT          759 a          712 c          572 g          899 t
ORIGIN      
        1 ctgaatccgc cgacttgctc aaagggcgcg tgaacggcgc ggcccgcaat cagttccgac
       61 aaattgttcc ccgcttcctt gacggcgagc gcgtcatgaa tgagatcatg cttgtcctca
      121 gaaacgtcga ggagcgaatc aatcccgagc atatccggca tggcaaaaaa atacaggtgc
      181 aaggcgtggt cgcggatcat catgctatac actgtcagtt tgcgcagaat caatgtctgc
      241 tcgctgatct tcacgcccag tgtcttttcg acggcttcaa tgcagcccgt gagatgggca
      301 atggagcacg ttccgcagat gcgcgagacc agctgcggca cggcggcata ggattgaccc
      361 ttgaccgcct ggttgaaaaa ccgcaagttt tccatgactt tcaagttcac ttcctgaagt
      421 tttccatccc ggattttgat atccagatcc gcattacctt caatcttgga aagtttgctt
      481 atttcgacgt ctaaattgca tgaatgcata tttttataga tatttttcaa atgtttcaat
      541 aaatttcccc tcatcgatcg gacaaccgga aatcgaatca tcaactttca ccgcgtcttt
      601 caaggccaga gcttttggca aaaaattata tttctcgaga tgcccttcga tttctttgag
      661 ctggctgatg ttgaaattgt tgcgctggcc ggacgggagc gccgtcatcg cgcaggtgcc
      721 catcgcgatc aaaattttgg agttggcccg gatcttttcg atgtcttctt tatccctttc
      781 acttgtcacc acgccttcca cgagagcaat atccagattt tttatctcca ccttgtcttt
      841 gatcgacaaa aaatacacca gttccatttt ttcgagccag ggaatcaatt tttggttgaa
      901 tatttccgtg agcaagatgc tgcacccctc gtcgcaagtg agcgaaaata tcccgacccg
      961 ttttttatta tttgtttctg ccatctctaa aatattcttt ttatcttctg caaagcgcga
     1021 tacaaattgt atttgcccgg cttgagtatt atatcataac atccgggaaa ttggatcggg
     1081 tttgtgtttg gggcgaagcc ggttttgaat ttggtaatcc ctgaccagct attgttcatt
     1141 gatctttgat cactgatcgc tgttttgatt ccgccaaaat catatctctc acaacccgct
     1201 tttttcgcat cctcgatttg ctgccattgc aaaagatacg gcgccatcaa attgcggtgc
     1261 tcattgtccg acgcgccatg catatatgtg gcggtttttc cgaaaaacaa aaccagattc
     1321 gccgcgatta ttttcccttc atattcggcg atatataatt tcaatatttc cgaaggaata
     1381 gtctcgaaca tctggcgata ataggcatct ggatgcgatg taattttatc tcgcttagat
     1441 gtaattttta ccaattgcaa aaacttatca atattttctt gtgttacatg tttcatgttg
     1501 tatgttacat gtacgcccct tttttctgct aattttatgt tatagcgcgt tttttgcttc
     1561 atcccggcta aaatttcttc ctcactcttc gaaatatcaa gcacgagaat ctctctcggc
     1621 tgcatatcaa cggatgattt ttttgttctc caatcttttg gcaaattatc ttgaattaat
     1681 tccaattctt cctcgctatt cggctcaatt cttatccagc caatgttatt ttctttagca
     1741 ttatcagtta aatcattcag aaattgtttg aaaattgaaa attgaaaatt gaaaattgaa
     1801 aaaaccggtc ctcgtggcgc gtaaaaataa tcgctgatta acggcaatct gtgtgcgatt
     1861 atatttgccg aaacagtcaa ttccccatct tcaccgttct ctgacaaatt atatgtcttt
     1921 cgtccgacgc tttcctgaaa tttccgccaa tactccgatt gcaaaaagcc cccatcgggg
     1981 gaattttgtt gaatgaaatt taaaagttta ttatccatat tcaaaatact atttccccaa
     2041 actcttcagc gccgctctca ctctttccga aacatccttc acgctttccg gcacggcctt
     2101 caaggcttcc cgcgcttgtg acacggaata gccgagagac gcgagcgctt caatcgcttc
     2161 ggaatcagcg cgcgccgctc cttgatcttc tcccggcaat tccgcaattt tgttcgagag
     2221 ctcaagaatt acccgctcgg cggtcttctt cccgacacca gaaacacgag tgagaatgga
     2281 agaatccccg gtgatgatgg ccgtgcggat tgttttgggc tcagcaatag tcaaaattcc
     2341 gattgccgct tttggcccaa tccccgaaat ggaaatcaag agctcaaaca ttccaagttc
     2401 atcaatcgtt aaaaatccgt acaaagacag cgaatcttcc cggacatatg tatgcgtgaa
     2461 tagctcaatt tcttccttgc cggcgatttt ccccatcgta aaatcggtga cgaatatctt
     2521 gtatccgacg ccgttcacat ccaccacggc atagtgatcc cgcaaaaacg caattttccc
     2581 tttgattttg gcaatcataa gttggtatct agtatatagt atctggtatt tggtattttt
     2641 ttacaatacc ccttactatt tacaaaaaag actggctatg atagaaatat aaggcgttat
     2701 taacaaattt ttaattttca attgtcattt tgcattttga tttttgcatt ttgattttga
     2761 acgaagtgaa ttatgccaat caaacaatct gccaaaaaat atctccgcgc ctcgaagagc
     2821 cgcgccgccc aaaactcaaa gatcaagaaa acttttcgcg tcgcgatgaa aaccgtgcgc
     2881 gagctcgccg cttcagaaaa ggccgacgag gcgaaaaagg ctttcccggc gatgcaaaaa
     2941 gc
//
LOCUS       DATKGS010000006         5565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_14484, whole genome shotgun sequence.
ACCESSION   DATKGS010000006 DATKGS010000000
VERSION     DATKGS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14484"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VMQ48_00100"
     CDS             complement(<1..225)
                     /locus_tag="VMQ48_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HUD08487.1"
                     /translation="MHNEKITTFAKTNFRGQERVFGIKEDDRRRHFYVVGKTGTGKTT
                     MIQNMAIQDIQNGRGVAIVDPHGEFAEEVLK"
     gene            complement(236..1504)
                     /locus_tag="VMQ48_00105"
     CDS             complement(236..1504)
                     /locus_tag="VMQ48_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08488.1"
                     /translation="MDFASFLKQYFETIGLLYSISWWWAGPIVLFYLFKILWTKYSFN
                     QYLSTLKWVMLEIKPPRNIERSPRTMEQVFAALHGVWSTPNYINKYVRGQWKQASLSF
                     EIQGVNGEMHFMVRCERRFRNFVESLIYSQYPEAEIQEVEDYTHSVPRNTPNAEWNLW
                     GVDFKKSKDSAYPIRTYKYFQEEVTKGMIDPLAELADVIAGLPPGQQIWLQFVVTPEH
                     DKDWQAAVKKVATKLAGKEVKSDPMIFFRFFSEIFDIISTSFLYMFHTVDEIAEKKKE
                     EQPLMFRLTPGEQEIIKAVEESIAKKGYRTKMRFIYVGRRDGFDRGFLSGVNGSIQQF
                     NDAMLNSLAHENETKTFANYIWVEPRMRFAQRKIFNRYLDRDFDGPTFVMNSEELATV
                     YHMPDMSVLSPAISRVEAKKGGAPINLPIE"
     gene            complement(1558..2670)
                     /locus_tag="VMQ48_00110"
     CDS             complement(1558..2670)
                     /locus_tag="VMQ48_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HUD08489.1"
                     /translation="MMTTENKFNLRVNTDIPQKIMVKSADIEIIVGDLTQKLGAKQKL
                     VLSGRFGISGQEPLTLEAIGKTLNITRERVRQIEADALNILKKGEKSRETEELISAAN
                     DIVKEKGGIVAAKTFADEVFEKYFGRKAKNDKERYLVEILFSVVGLKKIKGNKELEDA
                     WTSKDFDIKKFRNIASGIEKIFDDSKKLFSEEDLLKKIDIASQAVLEEKIEPEQILNY
                     LEISKKFGKNIFGQWGKAKWPLVRPRGVREKATLVLLNRQKPLHFREISQLIEKMGLS
                     DKKVHPQTVHNELIRDKRFVLVGRGTYALREWGFEEGTVKDIIVSLIKAAGGFLAKDV
                     IVKDVLKKRKVKKATIDVNLSDRKVFQKTKGGYQLK"
     gene            complement(2758..3645)
                     /gene="secF"
                     /locus_tag="VMQ48_00115"
     CDS             complement(2758..3645)
                     /gene="secF"
                     /locus_tag="VMQ48_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HUD08490.1"
                     /translation="MYNILGKRKYFYIFSGTLMLLSVIVLSTWKLKYGLDFTGGTLME
                     LQNVKAQSSDLKSVLSDAGINDAVVSPTQNNGFLVRYANSSDEKNQEVLAKLKEKFPD
                     VQNTSLDYVGPSISSDLKSNAFWALLFAVIGIAAYVAYAFRKVSRPVESWKYGVGAVV
                     ALVHDVLITVGIFAVLGHFLNIEVDSAFIAALLTILGFSVHDTIVVYDRTRENLLKSG
                     SGEKFDETVNRSLNETMARSINTSLTVLVTLLAIFIFGGQSIKTFTLALLIGITFGTY
                     SSIFVASALIVDWWKWEKK"
     gene            complement(3754..5469)
                     /gene="secD"
                     /locus_tag="VMQ48_00120"
     CDS             complement(3754..5469)
                     /gene="secD"
                     /locus_tag="VMQ48_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HUD08491.1"
                     /translation="MTVRRKIWIKFSLVVILFLITALVAYPKPIQKPAWLGNTLAKLK
                     INLGLDLQGGIHLIYKVDTSGVDPAKVPDALNGLQDVIEQRVNKFGVAEPVVETSKSG
                     GEQRLIVDLAGIKDINAAKDQIKETPKLEFKTIAAPAVDQSAAPTLSPEDQQYNDQQK
                     QKAEDLLKQIQGGADFSDLAKQNSEDPGSKDQGGDLGWNKRGVLVPEFDKVLFDSNLQ
                     PGQVYPQVVESDFGYHIIKLLEVRGDGDNMEVHAQHILLAKKTPPAPEQVQPTLVDTG
                     LTGKNLKNATADFDQQTGKPLVKIQFDNEGAKLFSSLTKENIGKPIAILLDGQIVSDP
                     IVQTQISDGNAIITGNFTTQGTKDIARKLNEGALPLPLEGPIQQQSVDATLGMNSFNM
                     AIKAGLIGIILVMIFMIIYYRFLGVVASAALIIYSSLMVAIFKLSSVTPWSITLTLAG
                     IAGFILSVGMAVDANILIFERTKEEIRKGRSIRSAIDEGFRRAWPSIRDSNISSIITS
                     LILIWFGTGFVKGFAITLLLGVLVSMFTAIVITRTILTAIIGGWAENKKSWIIVNSKN
                     EESTE"
BASE COUNT         1411 a         1365 c         1159 g         1630 t
ORIGIN      
        1 tttcagaacc tcttcggcaa attccccgtg cggatcgaca atagccactc cgcggccgtt
       61 ttggatgtcc tggatggcca tattctggat catcgtcgtc tttccggttc ccgtttttcc
      121 gacaacatag aaatgacggc gccggtcatc ttccttgatg ccgaaaaccc gctcctgtcc
      181 gcggaaattg gtcttggcaa aggttgtgat tttttcgttg tgcataaata atatattatt
      241 ctatcggcaa attgatcggt gcgccgccct tcttggcttc aacccgggag atggccggtg
      301 acaggactga catgtccggc atatgataga ccgttgccag ctcttccgaa ttcatgacaa
      361 atgtcggacc atcgaaatca cggtcgagat aacggttgaa aattttgcgc tgggcaaacc
      421 gcatccgcgg ctcgacccag atatagtttg cgaacgtctt cgtctcgttt tcatgagcca
      481 aagagttgag catcgcatca ttgaactgtt ggatggaacc gttgactccc gaaagaaatc
      541 cccggtcaaa accgtctcgc cgcccgacat agatgaaacg catctttgtc cgataacctt
      601 ttttcgcgat actttcttcg actgctttga taatttcttg ttccccgggc gtaagtctga
      661 acatcaaggg ctgctcttct ttcttttttt cggcaatttc atcgactgtg tgaaacatat
      721 acagaaaaga ggtgcttata atatcaaaaa tttctgaaaa aaaacggaaa aatatcatcg
      781 gatcgctttt cacttctttt cccgccaatt tggttgctac ttttttcacc gctgcctgcc
      841 aatctttgtc atgttcgggt gtgacgacga actgcagcca gatctgttgt ccaggaggaa
      901 gaccagcgat gacatccgcg agttccgcca gcggatcgat cattcctttc gtcacttctt
      961 cctgaaaata tttgtaagtg cggatgggat aggcactgtc tttggacttt ttgaaatcca
     1021 ccccccaaag gttccactcc gcgttcggcg tatttctcgg gacagaatgc gtataatcct
     1081 caacttcctg gatttccgcc tccggatatt gggaatagat caaggattcg acaaaattgc
     1141 ggaatcttcg ttcgcaacgg accatgaagt gcatttctcc gtttacgccc tgaatttcaa
     1201 aactcaaaga cgcctgtttc cactgtcctc tgacatactt attgatatag ttcggagttg
     1261 accaaacacc gtgcaaagcc gcgaaaacct gttccatcgt ccgcggactg cgttcgatgt
     1321 tgcgcggcgg cttgatttcc agcataaccc acttgagcgt actgagatat tgattgaaag
     1381 aatatttcgt ccacagaatt ttgaaaagat aaaacaaaac aatcggaccc gcccaccacc
     1441 aactgatgga gtacaaaagc ccgattgttt caaaatattg tttgagaaag gatgcaaaat
     1501 ccatattggt tgttaaattg attttatttt atctcgccct ttgataaggg aaaatctcta
     1561 tttcagctga tatccgcctt tggtcttctg aaagaccttc cgatccgaca gattcacgtc
     1621 aatggtcgct tttttcactt tgcgtttttt gaggacatct ttcacaatga catctttcgc
     1681 caaaaatccg ccggcggctt tgatgaggga aacgatgata tcttttacgg ttccttcttc
     1741 aaacccccat tcgcgcaagg catatgtccc ccgcccgacc aagacaaacc ttttgtcccg
     1801 gatcaattca ttgtgaacgg tctgcggatg gacctttttg tctgacagac ccattttctc
     1861 gatcagctgg ctgatttccc ggaaatggag aggcttttgg cggttcagca gcaccagagt
     1921 cgccttttcg cgcactccgc gcggacgcac aagcggccat ttggcttttc cccattgtcc
     1981 gaaaatattt tttccgaatt ttttggaaat ttcaagatag ttgaggatct gctccggttc
     2041 aatcttttct tccagcactg cttggcttgc aatatcgatc tttttcaaaa gatcttcctc
     2101 ggaaaaaagt tttttcgaat cgtcgaatat cttttcaatt ccacttgcaa tattgcgaaa
     2161 tttcttgata tcaaaatctt tgctcgtcca ggcgtcttcc aattctttgt ttcctttgat
     2221 ttttttgagg ccaaccactg aaaacaaaat ttcgacgaga tatctttctt tgtcgttttt
     2281 tgccttgcgt ccgaaatatt tttcaaaaac ttcatcggcg aatgttttcg ctgcgacaat
     2341 gccgcctttt tctttgacaa tatcatttgc agccgaaatc aattcttccg tttccctcga
     2401 cttttcaccc tttttgagta tattcagcgc atcggcttcg atttggcgca ctctttcgcg
     2461 agtgatgttc aaagtttttc cgatcgcttc aagcgtcagc ggttcttggc cggaaatgcc
     2521 gaaacggccc gagagcacca gcttctgttt ggcgcccaat ttttgcgtca aatcaccgac
     2581 gattatctcg atatcggcgg attttaccat tattttttgc ggaatatccg tatttacccg
     2641 caaattaaac ttgttttcag tagtcatcat ttttcccttt gattaaggct aatattagca
     2701 cgaattaatc ttagagtaaa atgataacat atgcaattta tgttgtcaag tagcgcccta
     2761 ttttttctcc catttccacc aatcgacgat caaagctgaa gcaacaaaaa ttgaggaata
     2821 ggtcccaaaa gtaatgccga tcaagagcgc gagcgtgaaa gttttgatcg attgtccgcc
     2881 gaaaatgaaa atggcaagca atgtcacaag cacggtcaag gatgtgttga tcgaacgcgc
     2941 catcgtttcg ttgaggctgc gattcaccgt ttcgtcgaat ttttcaccgg atccggattt
     3001 caaaagattt tcgcgcgtcc ggtcatagac gacaatcgta tcatgcacgg aaaaaccaag
     3061 gatcgtgaga agcgcggcga tgaaagcaga atcgacttcg atattcagga aatgcccgag
     3121 gacagcgaaa attccaaccg tgatcaggac atcgtggaca agagccacaa ccgcgccgac
     3181 tccatacttc cacgactcca ccggccgcga aaccttgcgg aaggcatagg caacgtatgc
     3241 cgcaatccca atcactgcaa atagaagcgc ccaaaaggcg tttgatttca gatcggacga
     3301 aatcgacggt ccgacatagt cgaggctggt attttggaca tccggaaatt tttctttcaa
     3361 ttttgccagc acttcctgat ttttttcatc gctggaattg gcataacgga caagaaaccc
     3421 attgttttgt gtcggcgaaa cgaccgcatc gttgattccg gcgtcactca ggacactttt
     3481 aaggtcactg ctttgcgctt tgacgttctg gagctccatc agcgttcctc ccgtaaaatc
     3541 caagccatat tttagtttcc acgttgaaag aacaatcacg ctcaaaagca tcagcgtccc
     3601 ggagaagata tagaaatatt tgcgtttgcc tagaatattg tacatgatcg taatattagc
     3661 gagcgaaaaa tttggagctt cgaaaatatt gcgtgtgccc gtccggcttt accggcgggt
     3721 agcctatttg agaagcccaa atttttcgcg agcctattcg gttgattctt cgttcttaga
     3781 attaacaatg atccagcttt tcttattctc tgcccatccg ccgatgatcg ccgtcaaaat
     3841 cgtccgcgta atcacaattg ccgtgaacat tgaaaccaaa actcccaaaa gcaacgtgat
     3901 ggcaaatccc ttcacgaatc ccgtcccgaa ccagatcaaa atgagtgagg tgatgatcga
     3961 cgagatattg ctgtcccgaa tactcggcca cgcgcgccgg aaaccttcat caatggcgct
     4021 gcggatgctc cgcccttttc tgatttcttc ttttgtccgt tcaaaaatca aaatattcgc
     4081 gtccaccgcc attccaaccg aaagaatgaa tccggcgatg ccggccagcg tcaaagtaat
     4141 tgaccaaggc gtgacggaag aaagcttgaa gatcgcgacc atcagagaac tgtatataat
     4201 caatgcggct gaagcgacaa cgcctagaaa gcgatagtaa atgatcataa atatcatcac
     4261 gaggatgatc ccgatcagcc ccgcttttat cgccatatta aacgagttca ttccgagagt
     4321 tgcatccacg ctttgctgtt gaattggtcc ctccaaagga agaggtagcg ctccttcatt
     4381 caacttacgg gctatatctt ttgttccctg cgttgtaaaa tttcctgtta taatggcatt
     4441 cccatctgat atttgagttt gaacaattgg atcgcttact atctgcccat caagcaaaat
     4501 tgctattggc ttcccaatat tttcttttgt tagactggag aataattttg caccttcatt
     4561 gtcaaattga attttgacaa gaggttttcc ggtctgttga tcgaaatctg ccgttgcatt
     4621 ctttaagttt ttaccagtaa gtcctgtatc aacaagagtc ggttgaactt gctccggcgc
     4681 aggcggcgtt tttttggcaa gcaaaatatg ctgggcgtgc acttccatgt tgtccccgtc
     4741 cccgcgcact tcaagcaatt taattatatg gtagccaaaa tccgactcga caacttgcgg
     4801 atatacctgc ccgggctgca aattgctgtc gaataaaact ttgtcaaatt ccggcacaag
     4861 tactccacgt ttgttccagc cgagatcccc accctggtct tttgatcccg gatcttccga
     4921 attttgcttg gccagatccg aaaaatcagc cccaccctgg atttgtttca ggagatcttc
     4981 agctttctgt ttttgctggt cattgtattg ctggtcctcc ggactcaggg ttggcgcggc
     5041 ggattggtca actgcgggcg ctgctattgt tttaaattcc agcttcggtg tttccttaat
     5101 ttgatctttt gcagcattga tatcttttat accagcaaga tcgacaataa gacgttgttc
     5161 tccccctgat ttagaagttt ccacaacagg ttccgctact ccaaacttat ttacacgctg
     5221 ttctataaca tcttgaaggc cattcaaagc atccggcact ttcgccgggt ccacgccact
     5281 ggtgtcgact ttgtagatta aatggatccc accttgcaga tccaggccaa gatttatttt
     5341 caatttcgcg agcgtatttc caagccacgc cggtttttgg atcggcttcg gatacgcaac
     5401 aagagccgtg atcaaaaaca agatcacgac cagagaaaac tttatccaaa tttttcttcg
     5461 gacagtcata ttggtatcat tatagcagaa agtggaaaaa aattccagtg ttaatttttt
     5521 tcaaaaaaga gagccaccaa gtctttgttt tccttctgga aaaaa
//
LOCUS       DATKGS010000007         3184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_86545, whole genome shotgun sequence.
ACCESSION   DATKGS010000007 DATKGS010000000
VERSION     DATKGS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86545"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VMQ48_00125"
     CDS             complement(<1..437)
                     /locus_tag="VMQ48_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08492.1"
                     /translation="MNILAIETSCDETAVALIEAKNNKFRVIEDFVSSQIKVHAPFGG
                     IVPNLAAREHLKNLPLLLKKTFKKHKISSKDIDLIAVTQGPGLIPALLIGTNSAKALA
                     YAWKKPLIGVHHIAGHIYANFVGNVKSQKSKVKSPEIKFPIPLL"
     gene            539..610
                     /locus_tag="VMQ48_00130"
     tRNA            539..610
                     /locus_tag="VMQ48_00130"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:572..574,aa:Glu,seq:ttc)
     gene            666..914
                     /locus_tag="VMQ48_00135"
     CDS             666..914
                     /locus_tag="VMQ48_00135"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HUD08493.1"
                     /translation="MKKSFSTKIKNISFPIVVEKDEDGFYVAECTIFQGCYSQGKSVD
                     EALKNIKEVIKLCLKEKENQEIVASYHPQELSLHNITL"
     gene            923..1141
                     /locus_tag="VMQ48_00140"
     CDS             923..1141
                     /locus_tag="VMQ48_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HUD08494.1"
                     /translation="MAKIPQISGKTAVKIFSKIGYRVVRQKGSHIRLHHPDKKPLTVP
                     DHKVLGKGLLRKLLRDSELSIEDFMKLM"
     gene            1317..1586
                     /locus_tag="VMQ48_00145"
     CDS             1317..1586
                     /locus_tag="VMQ48_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HUD08495.1"
                     /translation="MKKKELVDALVEKTQLAKKDVVMVLEEMAEIVKATVKKEAVEIP
                     GLVKVEKKHRAARMGRNPMTGEAISIPAKTVLKAKILKKLKDAVM"
     gene            1668..2411
                     /locus_tag="VMQ48_00150"
     CDS             1668..2411
                     /locus_tag="VMQ48_00150"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUD08496.1"
                     /translation="MSKKTYNGESIFLKGSNEVGILLLHGWSSYTDELLPLAKYLNSF
                     GFAVSAPFLRGHGTKPEDLFGVTWEDWLQDAKIELQKLEKYSSKIFIGGISMGGDLAM
                     LLSGEKSVAGLISLGSAVRYKFQPLVKITLFFMGLTKTHRKKYYPPWARKKMGDRKVY
                     PSYPVESAKEVVRLADATRAILPQVTKPILIMQSTTDFLVSKKSPQIVFEEVKSKIKK
                     IYWVKDGYHVFVEDRRVWKEIENFVKSNL"
     gene            2408..>3184
                     /locus_tag="VMQ48_00155"
     CDS             2408..>3184
                     /locus_tag="VMQ48_00155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08497.1"
                     /translation="MKIGIFTNNYLPNPYGVAGSVESFRKVFERLGHEVYIFAPRWKG
                     YEDKNPHVFRYPAIKTNIKIKFPIAIPYSRKISGIIDKLDLDVIHSQHPNLLGSAAMR
                     WARKKNIPLVFTWHTLYDQYAHFVPLVPQKLAAWLAIRNARNFTNRCDAVIVPTPSVG
                     EIIKKWGVRNENIVAIPTGVEEKQFESPDRESVRRKCGIEDNEILLFVMTRLTAEKNM
                     EFLVDAVSEIIRKNNSAKFMICGDGNIKEKLVEKVEEAGLV"
BASE COUNT          959 a          589 c          759 g          877 t
ORIGIN      
        1 aggaggggga taggaaattt aatttcggga cttttgactt ttgacttttg acttttgaca
       61 ttgccaacga agttggcgta aatatgcccg gcgatatgat ggactccgat caatggcttt
      121 ttccaagcat aggcaagagc tttcgctgaa tttgttccga tgagaagtgc cggaatgagt
      181 cccggacctt gggtgacagc gatcagatca atatcttttg aagaaatttt gtgctttttg
      241 aaagtctttt tgaggagcag cggcaagttt ttcagatgtt cgcgggcggc caaatttggc
      301 acaatgccgc caaaaggcgc gtgcactttg atctgcgaag aaacaaaatc ttcaatgact
      361 ctgaatttat tgtttttcgc ttcaatcaac gcaaccgccg tttcgtcgca ggatgtttca
      421 atggccagga tattcatgat gtaagtataa agtattacat aaaaagtata aagcctatct
      481 gcttttgttg actaatccgg tattttctgc tatcatttgc tttgtactat ccgtttttgg
      541 ccccatcgtc tatcggttag gacgttaggt tttcatccta aaaagagggg ttcgactccc
      601 cttggggcta cgattttgaa ataaatgcgg ttgaccggaa gtaatcaaac atatagaatg
      661 aatatatgaa aaaatcattt tcaaccaaaa ttaaaaatat ttcctttcca atcgtcgtcg
      721 aaaaagacga agacggtttt tatgttgccg aatgcacgat ttttcaggga tgctattctc
      781 aagggaagag cgtagacgaa gctctgaaaa atatcaaaga agttatcaag ctttgtctca
      841 aagaaaagga aaatcaggaa atcgtggcgt cctatcatcc tcaagagttg agcttgcata
      901 acataaccct ttgataaatt tcgtggcaaa gattcctcaa atttccggca aaacggccgt
      961 gaaaattttt tccaaaatcg gttatcgtgt tgtgagacaa aagggcagcc atatccgtct
     1021 gcatcatccc gacaaaaaac cgctaactgt tcccgatcac aaagttttgg gaaagggatt
     1081 attgagaaaa ctattgaggg attcggaact gtcaattgaa gattttatga agctgatgtg
     1141 aaaaatgcat ttatatcgta aagccgcctt tcggcgtttt tttgccccta aagaccgaac
     1201 tttataagat gaattcaaga atttaaagct ctcttgacaa aacattgcgg aaaagccagt
     1261 attttgttag tatttgaaag tcgaaagctc taaatacata attaatcaaa aaaaccatga
     1321 agaaaaaaga actcgtggac gcgctggttg aaaagactca gctggccaaa aaggacgtcg
     1381 tgatggtcct tgaggagatg gcggagatcg tgaaagccac ggtcaaaaaa gaagccgtcg
     1441 aaattcccgg ccttgtgaag gtcgagaaga agcaccgggc cgcccgcatg ggtcgcaacc
     1501 cgatgaccgg agaagcaatc agtattccgg ccaaaaccgt cctgaaggcg aagattctca
     1561 agaagctcaa agacgcggtt atgtaataga gaattactct ttcaaagatc ccccaaatcg
     1621 ggggattttt tgattttttg cggtcttctt gctacgctaa aagggcaatg agcaagaaaa
     1681 catacaatgg agaatcaatt tttttgaagg gtagtaatga agtgggaata ttgcttcttc
     1741 atggttggtc ttcctatacc gatgagcttt tgccgctcgc aaaatatttg aattccttcg
     1801 gctttgcagt ctcggcgcca tttttgcgtg gtcacgggac aaaaccggaa gatttgttcg
     1861 gtgtcacttg ggaagattgg ctccaagacg caaaaattga gttgcaaaag ctcgagaaat
     1921 attcttcaaa aattttcatt gggggcattt caatgggtgg ggatttggcg atgcttcttt
     1981 caggggagaa atctgttgcg ggtttgatat ctcttggcag cgcagtgaga tacaaatttc
     2041 aaccgttggt gaagataact ctttttttca tgggtttgac gaaaacacac cgaaaaaaat
     2101 attatcctcc ctgggcgcga aaaaaaatgg gagatagaaa agtatatcct tcatatcccg
     2161 tagaaagtgc aaaagaagtg gtgcgattgg ctgatgcgac gcgggctatt ttgccgcagg
     2221 ttacaaagcc gattcttatc atgcagtcga cgaccgactt cttggtttcc aaaaaatcgc
     2281 cgcagattgt ttttgaggaa gtgaaatcaa agataaaaaa aatatactgg gtgaaagacg
     2341 gatatcatgt ttttgttgaa gatagaaggg tgtggaagga gatagaaaat tttgttaaaa
     2401 gcaatttatg aaaattggta ttttcaccaa caactatctg cccaatccct atggcgtggc
     2461 gggctccgtc gaaagttttc ggaaagtttt tgaacggctt ggccacgaag tgtatatttt
     2521 cgcgccgcgc tggaaaggat atgaagacaa aaatcctcat gtttttcgct atccggcaat
     2581 caagacaaat ataaagatca aatttccgat tgcgattcca tattcgcgaa agatcagcgg
     2641 gattattgac aaattggatt tagatgtcat ccattcccag caccccaatc ttcttggaag
     2701 cgcggcgatg cgctgggcac gcaaaaaaaa tatcccgctg gttttcactt ggcatactct
     2761 ctatgatcag tatgcgcatt ttgtgccgct tgttccgcaa aaattggccg cctggctggc
     2821 aatccgaaac gcgcgaaatt ttactaatag atgcgatgca gtgattgttc cgacaccgtc
     2881 ggttggggag atcatcaaga aatggggggt aaggaatgaa aatatcgttg ctattcccac
     2941 cggggttgaa gaaaagcaat ttgagagtcc tgatcgggaa agcgtgagaa gaaaatgcgg
     3001 catcgaagac aatgaaattt tgctttttgt gatgacgcgc ctcacggcgg aaaaaaatat
     3061 ggaatttttg gttgacgcgg tttcggaaat tattcgaaaa aataattcgg caaaatttat
     3121 gatttgcggc gacggaaaca tcaaagagaa gttggtagaa aaagttgagg aggcggggtt
     3181 ggtg
//
LOCUS       DATKGS010000008         3469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_100951, whole genome shotgun sequence.
ACCESSION   DATKGS010000008 DATKGS010000000
VERSION     DATKGS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100951"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..907
                     /locus_tag="VMQ48_00160"
     CDS             191..907
                     /locus_tag="VMQ48_00160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUD08498.1"
                     /translation="MKKIIIALVVIILAGIVFLLARNMFRKNNAPAPSSTVSTNIPAV
                     ENQTSSQSVPAANTPPDNQNTTISQNPNGFQAPLDRAAERVTKKPFGIYITPKTSPVQ
                     PEKFQGYHTGTDFEIFPEELNADVPVRAICSGKIAMKRSASGYGGILVQNCMFNNQPI
                     TVIYGHLKLASIGNIAGDNLNAGDEIGFLGKAYSSETDGERKHLHLGIHKGSAVSILG
                     YVQSQSELSGWIDPCSLVCK"
     gene            complement(926..1588)
                     /locus_tag="VMQ48_00165"
     CDS             complement(926..1588)
                     /locus_tag="VMQ48_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08499.1"
                     /translation="MEQNPTGQGEQQPPATPPTNQPVQKKSSSKIVLIIVAIVVVLGI
                     VALVGGYFVMKSIRTKISQKIGQSIGENMAEKAIEKATGQKADVNTDNNSVSIKTDSG
                     TFAASESGTIKLPSDFPSDVFTYPDAKITFATSTPANAADGTKASFMIGYTVNQSVAD
                     VVAKYKAEMAKNGWTVGTEANYGAMMIDFKKGNKDVSVTVGDSQGDQTGSTGVSVTGS
                     EN"
     gene            1751..1975
                     /locus_tag="VMQ48_00170"
     CDS             1751..1975
                     /locus_tag="VMQ48_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08500.1"
                     /translation="MCNEHEKGFEEMDEAMKTCCANWPKKFKKEFMMAKMKKKEKILE
                     AKLEFLREMRRLAEKSEMGEEKESEEEEKA"
     gene            complement(2024..2401)
                     /locus_tag="VMQ48_00175"
     CDS             complement(2024..2401)
                     /locus_tag="VMQ48_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08501.1"
                     /translation="MNHDKKIEERISQYQALGKEDKSVDVAKLMIFSLNEKGNVVSSK
                     KKRWAYIVSLGLPPIGFLLALKYYFDDEDDARHVANVCVVITLAALLMVWISFRMLSS
                     GSAGTEINQIQQINPQDLQDLMK"
     gene            complement(2440..3432)
                     /gene="rlmN"
                     /locus_tag="VMQ48_00180"
     CDS             complement(2440..3432)
                     /gene="rlmN"
                     /locus_tag="VMQ48_00180"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HUD08502.1"
                     /translation="MNLSKLEEILKSQPAFRLKQAKKAVFFDLAEDWSEATTISKDLR
                     EKLRKEVPLEIKGKILVSKNGRTVKAALELEDGLKIETVLMKHTDKRNTVCVSSQVGC
                     PLGCLFCVTGTMGFKRNLTSSEIVEQVLFFARYLKKDKEKITNVVFMGMGEPFLNWEN
                     VKKAIEILNDDNCFGIGARKISVSTAGITEGIERLAEDFPQINLAISLHAPDDKLRGE
                     LMPVNQKYPLEKILKSVDEYIEKTNRKVMFEYVMLKDVNDSEEQAKKLAKRLKYPLYM
                     VNLIAYNPNGRFESSSPERIKKFKAVLEKAGIFATQRYRFGTDIEAACGQLAGK"
BASE COUNT          926 a          830 c          710 g         1003 t
ORIGIN      
        1 cgcatccccc aaagatatgt tcggattgtc atttggctgt ttgttttacc tatatcattc
       61 tagcaggaaa aaatattttt ggcaagcaaa atccccccga ttttgaggct tgtataaagc
      121 ctaattaggg ataatcccgg atttgcaata taatggatac tatatatgta agaaatagta
      181 aataaattct atgaaaaaaa ttattattgc cttggtcgtc atcattctcg ccggaatcgt
      241 ttttcttttg gcacgaaata tgttccgaaa aaacaatgct ccggctccgt ccagtactgt
      301 ttcaacaaat attcccgcgg ttgaaaacca aacatcttct caaagcgtac ccgccgcgaa
      361 tactccgcct gacaatcaaa atacgaccat ctctcaaaat ccaaacggat ttcaggcgcc
      421 gctcgaccgc gccgctgagc gagtgacaaa gaagccattt ggcatataca tcactccgaa
      481 aacttcgcct gtccagccgg aaaaattcca gggctaccac accggaacgg attttgaaat
      541 ttttccggaa gaactcaatg ccgacgtgcc cgttcgcgct atttgcagcg ggaaaatagc
      601 catgaaaaga tccgcctcgg gatatggcgg gatactcgtc caaaattgca tgttcaataa
      661 tcaaccgatc accgtgatct acggccattt gaagctcgcc agcatcggaa atatcgccgg
      721 cgacaatttg aacgccggcg atgaaattgg atttttggga aaagcctatt cttccgaaac
      781 ggacggcgag cgaaagcatc ttcatctcgg cattcacaaa ggcagtgctg tcagtattct
      841 gggctacgtc cagtcgcaat ccgaactttc cggatggatc gatccgtgtt cgcttgtttg
      901 caagtagatt tcttttttat aaagcctagt tttccgagcc agtgacggaa actcctgtgg
      961 atccggtctg gtcgccttga ctgtcgccaa cggtcaccga aacatcctta tttcctttct
     1021 tgaaatcgat catcatcgct ccatagttgg cttccgttcc cactgtccag ccgtttttgg
     1081 ccatttccgc tttatatttc gcaactacat ccgcaacgct ttggttcacg gtatacccga
     1141 tcataaagct ggcttttgtc ccatcagctg cgttggccgg tgtcgaagtg gcaaaggtta
     1201 tcttggcatc aggataagtg aaaacatcgg agggaaaatc tgaaggcagc ttgattgttc
     1261 ccgattcaga agcggcaaac gttcccgaat ctgtcttaat actcacagaa ttattgtccg
     1321 tgttcacatc ggctttttgg ccggtggctt tttcaatcgc cttttccgcc atattttctc
     1381 caattgactg gccgattttt tgcgagatct ttgtccggat gcttttcatc acgaaatatc
     1441 cgcccacaag agcaacaatg cccaaaacga cgacgattgc gacaatgatc agcacgattt
     1501 tgctgctgct ttttttctgc accggttgat ttgttggcgg cgtggccggc ggctgctgtt
     1561 ccccttgccc tgttggattt tgctccattt tgttttgctt aattgttagc gttttttcct
     1621 ggcaatattc tatcacaaat cgacttttct ctcaatttct gaaaaagaaa aatatttttg
     1681 aaatcaaaat cattttgcgt tcgtactcaa atatgaaagg tcggcaaaaa taattttaag
     1741 actcaaaact atgtgcaacg aacacgaaaa aggtttcgag gaaatggatg aggcgatgaa
     1801 gacgtgctgc gcgaattggc cgaagaaatt caaaaaggaa tttatgatgg ccaagatgaa
     1861 gaagaaagaa aagattctcg aggccaagct tgaattcctc cgcgaaatgc gccgcctcgc
     1921 ggaaaaatca gaaatgggtg aagaaaaaga aagcgaagag gaagaaaaag cttgaaggca
     1981 aaaataaatc actccaaaag ctggggtgat ttattttatg tttttatttc atcaaatcct
     2041 gcaaatcctg cggattgatt tgctgaattt ggttgatctc ggttccagca gacccagaag
     2101 aaagcatcct gaaggaaatc caaaccatca gcaatgctgc aagcgtgatg acaacgcaca
     2161 cattcgccac gtgccgggcg tcatcttcgt catcgaaata gtatttgagc gcaagcaaaa
     2221 agccgatcgg cggaaggccc aaggaaacaa tatacgccca gcgttttttc ttggaagaca
     2281 cgacattccc cttttcgttg agggaaaaaa tcatcaattt cgccacgtca acactcttgt
     2341 cttccttccc cagcgcctga tattgagata tgcgctcctc tatttttttg tcatggttca
     2401 tagattcaat tataccaaaa aaccgtttat gggacgattt tattttcccg ccagttgtcc
     2461 gcacgccgct tcgatgtccg tgccaaagcg atatctttgg gtggcgaata tgccggcttt
     2521 ttctaagacc gccttgaatt ttttgatcct ctcgggcgag gatgattcaa atcggccatt
     2581 cggattgtag gcgatcagat tgaccatata taaaggatat ttcagccgct tcgccagttt
     2641 tttcgcctgc tcttccgaat cattcacgtc cttaagcatc acgtattcaa acatcacttt
     2701 acgattggtt ttttcgatat attcgtccac agatttcaga attttttcaa gcggatactt
     2761 ctgattgacc ggcatcagct cccctcgcaa tttgtcatcc ggcgcatgga gcgagatcgc
     2821 caggttgatc tgcggaaaat cttcggccag tctttcaatt ccttccgtga ttcccgccgt
     2881 tgaaacggaa atttttcgcg ctccgatccc gaagcaattg tcatcattga ggatttcaat
     2941 cgcttttttc acgttttccc aattcaaaaa aggctccccc attcccatga aaacgacgtt
     3001 ggtgattttt tctttatctt ttttgagata tcttgcgaaa aacaaaacct gctcgacgat
     3061 ttccgaagaa gtgagatttc tcttgaagcc cattgtcccc gtcacgcaaa acaaacatcc
     3121 caaagggcat ccaacttgag atgaaacaca gaccgtattg cgtttgtccg tatgtttcat
     3181 gagaactgtt tcgattttca aaccgtcttc aagctcaagc gccgctttca ctgttcgtcc
     3241 gttttttgaa acaagtattt ttcctttgat ctcaagcggc acttcttttc gcaatttttc
     3301 gcgcagatcc ttcgaaatcg tcgttgcctc actccaatcc tcggccaaat caaaaaaaac
     3361 tgctttcttg gcttgcttca accgaaacgc cggctgcgat ttcaaaattt cctcaagttt
     3421 tgagagattc attcaatatt gggatattat caaaataagc taagaattt
//
LOCUS       DATKGS010000009         2482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_144079, whole genome shotgun sequence.
ACCESSION   DATKGS010000009 DATKGS010000000
VERSION     DATKGS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144079"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VMQ48_00185"
     CDS             <1..246
                     /locus_tag="VMQ48_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08503.1"
                     /translation="GNAEETVNPRTEEMDTEHSTFPVPFWYLNPKNHKQKSESEILNA
                     KNNIGGLLSDVAPTILAVMDIPVPPEMTGQNLMEVLI"
     gene            complement(286..1011)
                     /locus_tag="VMQ48_00190"
     CDS             complement(286..1011)
                     /locus_tag="VMQ48_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HUD08504.1"
                     /translation="MEAILLSIATFVSTYSGGLLAVKLKNKLHLIMGFTAGVILGVVF
                     FEIFPEIIEQIKERDIAPTGVMVALLVGFLVFHILEKAILIHHAHEGDYAEHIHPQVG
                     VLSALALIGHSFLDGFGIGLGFQASPAVGLLVAVAVVSHDFTDGMNTITMMLSHENSL
                     KRAKVFLLFDALAPVFGVLATLFFKLPPTFLILYLGFFAGFLLYIGASDILPQAHMKK
                     SSYQLMSLTVLGALLVFAISRFV"
     gene            complement(1015..1710)
                     /locus_tag="VMQ48_00195"
     CDS             complement(1015..1710)
                     /locus_tag="VMQ48_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HUD08505.1"
                     /translation="MFHEKRKNLILTADDFGKSELANLSILRLAKSGKLDRVSVMSDG
                     DFAPGEIEELAATGVKLDIHLELSWQKKRRGKMRDNTARQGIVFLMNHLRSSQRKKIR
                     EDWKKQIEKFHILTGRYPDGINSHEYVHLFPPYFKIALSLASEFDIPFVRFGRHGFLG
                     KRNMANLVLNNLRKWNKKYFLEAKLDSSDHFVSLDWIANFDKFFRHIPEGKIEIACHP
                     EREEEFELIDKYF"
     gene            complement(1717..1799)
                     /locus_tag="VMQ48_00200"
     tRNA            complement(1717..1799)
                     /locus_tag="VMQ48_00200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1764..1766),aa:Leu,seq:taa)
     gene            complement(1812..1882)
                     /locus_tag="VMQ48_00205"
     tRNA            complement(1812..1882)
                     /locus_tag="VMQ48_00205"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Cys,seq:gca)
     gene            2097..>2482
                     /locus_tag="VMQ48_00210"
     CDS             2097..>2482
                     /locus_tag="VMQ48_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08506.1"
                     /translation="MAKKKAKRKTKKTRKVQNTPKNKAMRGTSAPECFLFGLIISGAI
                     LFSFLSWQSAKQVSTSFSTMNISNEKNVFQEKINDLVGGYPIEKMTPYISHKNKKVAS
                     FLVAIAKKESNWGRRSPKKNGKECFN"
BASE COUNT          781 a          571 c          508 g          622 t
ORIGIN      
        1 ggaaacgccg aggaaacggt caatccccgg accgaggaaa tggatactga gcattccacc
       61 ttcccggttc ctttttggta tttgaatccc aagaaccaca aacaaaaaag cgaatcagaa
      121 attctgaacg ccaaaaacaa cattggcgga cttttgagcg atgtcgcacc gacaattctg
      181 gccgtgatgg atattcctgt cccaccagaa atgaccggcc aaaatttgat ggaagttttg
      241 atatgatgtg tagagacgca aaatcttacg tctctacttt tacttttata cgaagcgaga
      301 aatcgcaaaa accaacaagg ctcccaaaac cgtaaggctc atcaattggt atgagctttt
      361 tttcatatgg gcttgaggaa ggatatcgct tgcgccgata tagagcaaaa atcccgcaaa
      421 aaaaccgaga taaagaatca agaaagtcgg gggcagcttg aaaaaaagag tggccagcac
      481 gccaaacacc ggcgccaacg cgtcaaaaag gagaaaaacc tttgctcttt tgagactatt
      541 ctcgtgagaa agcatcatcg tgatcgtatt cattccgtcc gtgaaatcat gagacacaac
      601 cgctactgcg acaagcaggc cgaccgcggg actcgcctgg aaaccgagcc cgatgccgaa
      661 cccgtccaag aagctgtgcc cgatgagcgc gagagccgac aaaactccga cctgcggatg
      721 gatatgctcg gcatagtcgc cctcgtgagc gtgatgaatg agaattgcct tctcaagaat
      781 atgaaaaacc aaaaaaccga caagcagcgc aaccatcact cccgttggcg cgatatctcg
      841 ctcttttatc tgctcgatga tttcgggaaa gatttcaaaa aaaaccacgc ccaaaatgac
      901 tccggccgtg aaccccatga tgagatgcaa cttgtttttc agctttactg ccaaaagccc
      961 tccggaatag gtggaaacaa aagttgcgat tgaaagcaat atggcttcca tgtcttagaa
     1021 atatttatca attaactcaa attcttcttc tctttccgga tgacaagcaa tttcaatttt
     1081 tccttccgga atatgtctga aaaatttatc aaagtttgca atccaatcaa gacttacaaa
     1141 atgatctgat gaatccaatt ttgcttcaag aaaatatttt ttgttccatt tgcgcaggtt
     1201 gtttaaaacc agattagcca tgttcctttt tcccaaaaaa ccgtgcctgc caaaccggac
     1261 gaaggggata tcaaattcgg atgctagact caacgctatt ttgaaataag gcggaaaaag
     1321 atgaacatat tcgtgtgagt tgatcccgtc cggatatcgg ccggtcagga tgtgaaattt
     1381 ttcaatttgt tttttccaat cttctcgaat tttttttctc tggctcgagc ggagatgatt
     1441 catcaaaaaa acaatcccct gccgcgcggt gttgtctctc atttttcctc tccttttttt
     1501 ttgccaagaa agctcaagat gaatatccag cttcactccc gttgccgcaa gttcttcaat
     1561 ttctcccggc gcaaaatctc catcactcat gacggaaacc cggtcgagct tccccgattt
     1621 cgccagccgc aaaatactca aattcgcgag ctcgcttttc ccaaaatcat cggcagtgag
     1681 gatcaaattt ttgcgttttt cgtgaaacat aaatcgtgcc cagggcgaga cttgaactcg
     1741 caagggattg ctcccgaggg attttaagtc ccttgtgtat accattccac cacctgggca
     1801 aaataattta gaggtccgga gcggatttga accgctgtac aaggttttgc agacctttgc
     1861 ctaaccgctc ggccaccgga ccatctactt tcaactgata taaaaattat cattaatagg
     1921 gcataaggtc aataaaaaga gacgatccgg cactgtaaaa tgatgtcaat ggttgagaat
     1981 gaatatcgtt acaaagcact tgctccggag ccaaaaatgt gctaaaatat aaccagtaat
     2041 aaagaaacgg gtggaagccc gttttttgcg tgaaaatacc acaaatatct tcatatatgg
     2101 caaagaaaaa agcaaaaaga aaaacaaaga agacaagaaa agtccagaac acaccaaaaa
     2161 ataaggccat gcggggaaca tctgctccgg agtgtttttt gtttggccta atcatatcag
     2221 gtgcgatttt gttttcattt ttgtcttggc agagcgcgaa acaagtttca acctcatttt
     2281 cgacgatgaa tatttcaaac gaaaaaaatg tttttcagga aaagatcaac gacctggtcg
     2341 gcggatatcc gatcgaaaaa atgacaccct atatttctca caaaaataaa aaagtcgcct
     2401 cttttctcgt cgccatcgcg aaaaaagaaa gcaactgggg gcgccgttca ccaaaaaaga
     2461 acggaaaaga atgtttcaat ta
//
LOCUS       DATKGS010000010         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_144101, whole genome shotgun sequence.
ACCESSION   DATKGS010000010 DATKGS010000000
VERSION     DATKGS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144101"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VMQ48_00215"
     CDS             <1..1701
                     /locus_tag="VMQ48_00215"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HUD08507.1"
                     /translation="RRHFYVVGKTGTGKTTMIQNMAIQDIQNGRGVAIVDPHGEFAEE
                     VLKAVPASRINDVIYFNPADQEWPIAFNVMEKVDTEYRHLIASGLVGVFKKIWADSWG
                     PRLEYILRNAILALLEYPGSTLLGVTRILVDKNYRERVVEKISDPVVKSFWVDEFTKW
                     NDRVLQEVISPIQNKVGQFLSSALIRNIVGQTKSAFNIRDLMDQEKVIIMNLSKGRIG
                     EDNSALLGAMMITKIQLAAMARVDVPEEERKDFYLYVDEFQNFATDSFANILSEARKY
                     RLNLILAHQYIGQLTTDTSTKVRDAVFGNVGTIATFRIGAEDAEFLEKEFEPIFMIQD
                     LVNLPNRNIYIKLMIDGLTGSAFSAATLPPIDLSKTTANAEKVIKVTRERYGTSREVV
                     EEKIARWSGMLTEKEMKMNADKPEIIRIKPDNMKNDNTPARTAVPARNVQAVQKTEDN
                     RTMYPAKCATCETDIQVPFKPDASRPTFCKDCLKDYQRQQARVQQVRSARAEKPAEPK
                     KYGQNLSNSYSPPRSISLRDAAKMQPQNFRRERIEKPRKQVDLAGVRSLIKESIDSKK
                     "
     gene            1713..>2123
                     /locus_tag="VMQ48_00220"
     CDS             1713..>2123
                     /locus_tag="VMQ48_00220"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HUD08508.1"
                     /translation="MPNRKILIALSGGVDSAVSAALLKKQGFDVFGVHFCFFDPPKAG
                     KADGSLKKAKLVAEKLNIPLEIVDARKEFKKKIIDYFIQAYKKGITPNPCVVCNREMK
                     FALLFEMLKKYKADFMATGHYARIKREFSIFNFQF"
BASE COUNT          596 a          486 c          568 g          473 t
ORIGIN      
        1 cgccgtcatt tctatgttgt cggaaaaacg ggaaccggaa agacgacgat gatccagaat
       61 atggccatcc aggacatcca aaacggccgc ggagtggcta ttgtcgatcc gcacggggaa
      121 tttgccgaag aggttctgaa agcggttccg gcgagccgga tcaatgatgt gatctatttc
      181 aacccggccg accaggagtg gccgatcgcg ttcaacgtga tggaaaaagt ggatacggaa
      241 tacaggcact tgatcgcttc aggcctggtc ggggttttca aaaaaatctg ggcggattcc
      301 tgggggccgc ggctcgaata tattttgcga aatgccattc ttgcgcttct cgaatatccg
      361 gggagcacgc tcttgggcgt gacgcgcatc ctcgttgaca aaaactaccg cgagcgggtg
      421 gtggaaaaaa tcagcgatcc ggtggtgaag tctttctggg tggacgagtt cacgaaatgg
      481 aacgatcggg ttttgcagga agtcatttct ccaatccaga acaaggtcgg ccagtttctt
      541 tcaagcgcgc tcatccgcaa cattgttgga cagacaaagt cagctttcaa tatccgcgat
      601 ctcatggacc aggaaaaagt catcatcatg aacctttcca agggacggat cggtgaagac
      661 aatagcgcgc tgctcggggc gatgatgatc accaaaatcc agctggcggc gatggcgcgg
      721 gtggatgttc cggaagaaga gcggaaagat ttctatcttt atgtcgacga gtttcaaaat
      781 tttgcgacgg actctttcgc gaacatcctt tccgaagcgc gaaaatatcg tctcaatttg
      841 atcctggccc atcaatatat cggccagctc acgaccgaca catcgacaaa agtgcgggat
      901 gctgtttttg gaaacgtggg cacaatcgca acctttcgga tcggtgcgga ggatgctgaa
      961 tttctggaaa aagaatttga gccgatattt atgatccagg atctggtcaa tcttcccaac
     1021 cgcaatatat acatcaagct gatgattgac ggactgaccg ggagcgcttt ttcggcagcc
     1081 actttgcctc cgattgatct ttccaaaacg acggccaacg cggaaaaagt catcaaagtg
     1141 acgcgcgaac ggtatgggac gagccgggaa gtcgttgaag aaaaaatcgc ccgctggagc
     1201 gggatgctca cggaaaaaga gatgaaaatg aacgcagaca agccggaaat aatcagaatt
     1261 aagccggata atatgaaaaa tgacaatacg cccgcgcgca cagcggtgcc cgcgagaaac
     1321 gtacaagccg tccaaaaaac ggaggataat cgcacgatgt atccggcgaa atgcgccacc
     1381 tgtgaaacgg atattcaggt ccctttcaag cccgatgcct cgcggccgac attctgcaag
     1441 gactgtctca aagactatca gcgtcagcag gcgcgtgttc agcaggttag aagcgcgaga
     1501 gcggaaaaac cggccgagcc caaaaagtat ggccaaaatc tttccaatag ctattctccg
     1561 ccaagatcaa tctcgcttcg cgatgccgcg aaaatgcagc cccagaattt ccgtcgcgag
     1621 agaatcgaga aaccgcgcaa gcaagtggat ctggccggtg tgcgaagctt gatcaaagaa
     1681 agtattgatt cgaaaaaata atttttggaa taatgcctaa taggaaaatt ctcattgcgc
     1741 tttccggcgg ggtggattca gcggtttcgg cggccttgct caaaaagcag ggctttgatg
     1801 tttttggagt gcatttttgt tttttcgacc cgcccaaggc gggcaaggcg gacgggagtt
     1861 tgaaaaaggc gaaactagtt gcagaaaaat tgaacattcc gcttgaaatt gtcgatgcgc
     1921 gaaaagaatt caagaaaaag ataatcgatt atttcatcca ggcatacaaa aaagggatca
     1981 cgcccaatcc gtgcgtggtt tgcaatcggg agatgaaatt tgctttgctg tttgaaatgc
     2041 tcaagaaata taaggcggat tttatggcca ccgggcacta tgcccgcata aagcgggaat
     2101 tttcaatttt caattttcaa ttt
//
LOCUS       DATKGS010000011         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_144368, whole genome shotgun sequence.
ACCESSION   DATKGS010000011 DATKGS010000000
VERSION     DATKGS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144368"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VMQ48_00225"
     CDS             <1..352
                     /locus_tag="VMQ48_00225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUD08509.1"
                     /translation="SFRYATRGLKYVLKNEQNFQIEILGGLFVVILMYLFPTRSLEKI
                     ALYIVIFAVLVMELINTIFERVVDMLKPRVHPYAQLVKDVMAAAVLLSSVGAVIVGVI
                     IFWPYFQDLFLQGM"
     gene            378..1631
                     /gene="ftsA"
                     /locus_tag="VMQ48_00230"
     CDS             378..1631
                     /gene="ftsA"
                     /locus_tag="VMQ48_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUD08510.1"
                     /translation="MSKGDIIIGLDVGSSNVRAVILQKFEEEEKPRVVGVGVAPSAGM
                     RRGVVADVEETIKSISTAVGNAERTSGIPVSRAIVSIGGSHISSQESQGVVATGKADG
                     EVTGDDLARVLSAAETISLPSNTEILHVMPRNYSVDDQKNVKDPLGMNGIRLEVNSLL
                     VLGSTPFIKNLSKCVYQAGVEIDDLVFAGLAASKASLNKRQKELGVVLVDMGGGTTNF
                     IVFEEGELVQLGVVPIGGGHITNDIAIGLRTSIDVAEKVKINYGSALPDEVSKKEQIN
                     LAEIDQNEEGEISRHHIAEIVEARLEEIFTLVDKELRKTGRSGMLPAGAVLVGGGAKL
                     PGAVDVAKKILRLPAQTGFPADLGGIVDKVDEPGFVTAVGLALWGLEDMPVRSTSMKM
                     PGLNNMPNMGKTVEKMKGWFRKFLP"
     gene            1891..3102
                     /gene="ftsZ"
                     /locus_tag="VMQ48_00235"
     CDS             1891..3102
                     /gene="ftsZ"
                     /locus_tag="VMQ48_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HUD08511.1"
                     /translation="MAEVKPEIETFARIKVVGVGGSGGNAISRMIDHKLRGVEFVAIN
                     TDAQDLHHSKAAEKIHIGKNLTKGLGAGMNPEIGRQAAEENRDEIHEILKGADMVFVT
                     CGMGGGTGTGAAPIVAEAAKEAGALTIAVVTKPFSFEGAQRKAIAAEGLENLRERVDA
                     LITISNDRLLQIIDRKTTLINAFRVVDDVLRQGVQGISDLISKPGIVNVDFADVKAIL
                     QDSGSALMGIGTASGENRSAEAAKLAINSPLLEFSIDGAKGILFNVSGGPDMTMLEIN
                     EAANVITESIDPNAKVIFGASVDESAKKGELTITVVAAGFDAEMVNEPLSSMQPVSMS
                     RETKPAIEEPMISSSSSFSDEEKPKFIIEEKMAPKPKRQINFESKTSTIGEEEDEELE
                     IPAFIRRKMKK"
     gene            3284..>3549
                     /locus_tag="VMQ48_00240"
     CDS             3284..>3549
                     /locus_tag="VMQ48_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HUD08512.1"
                     /translation="MIDSRESTDGKVIRRRRECLKCQKRFSTYEQLELLNFVVVKKDG
                     RREDYKREKLEKGIRKALEKRPIDGKKIEEAIDEIEYKLQLDKN"
BASE COUNT          983 a          746 c          971 g          849 t
ORIGIN      
        1 aagcttccgc tatgctacgc gcggcctgaa gtacgtcctc aaaaacgaac agaatttcca
       61 aattgagatt ctggggggtc tttttgtggt cattttgatg tatcttttcc cgacgcggtc
      121 tctcgaaaaa atcgctttgt atattgtcat tttcgcggtg ctggttatgg agctgatcaa
      181 tacgatattc gaacgggtgg tggatatgct gaaaccccgc gtccatccct atgcccagct
      241 tgtgaaagac gtgatggcag cggcagtctt gctttcttcg gtcggagcgg tgattgtcgg
      301 cgtgatcata ttctggccgt acttccaaga cctgtttttg caaggaatgt agtttttctg
      361 tataattatc atgcattatg tctaaaggag atatcatcat tggtctcgat gtcggctcgt
      421 cgaacgtgcg ggcggtgatt ttgcaaaaat tcgaagaaga agaaaaaccg cgcgtggtgg
      481 gggtgggtgt ggcgccttcc gccgggatgc gccggggagt tgtggctgat gttgaagaaa
      541 caatcaaatc catctcaacc gccgtcggaa acgccgagcg gacttccggt attcccgtct
      601 cgcgggcgat cgtgagcatc ggaggcagcc atatcagctc gcaggagagc caaggggtgg
      661 tggcaacggg caaagccgac ggcgaggtca ccggcgatga tttggcgcgg gttttgagtg
      721 cggcggaaac gatttccctt ccttcgaaca ctgaaatttt gcacgtgatg cctcggaatt
      781 attctgttga cgaccaaaaa aacgtgaaag atccgctggg aatgaacggg atccgcctcg
      841 aggtgaattc gcttttggtc ctgggttcga ctccttttat caaaaatctt tccaagtgcg
      901 tctatcaagc cggagtggag atagacgacc ttgtttttgc gggtctggcg gcttcaaaag
      961 cttcgctcaa caaacggcag aaggaattgg gggtagttct tgttgatatg ggcggcggga
     1021 cgacaaattt cattgttttc gaggaaggag agttggtcca gctgggggtt gtcccgatcg
     1081 gcgggggtca catcacaaat gatattgcca tcggtcttcg aacctcgatc gatgtggcgg
     1141 aaaaagtgaa gatcaactat ggctcggcat tgccggatga ggtcagcaag aaagaacaaa
     1201 tcaatcttgc cgaaatcgat caaaacgagg agggggaaat ttcgcgccat cacatcgcag
     1261 aaatcgtcga agctcgtctc gaagaaatat tcactttagt tgacaaggag ctcagaaaaa
     1321 caggccgttc aggaatgctg ccagctggag cggttcttgt tggaggcgga gccaaacttc
     1381 ccggcgcggt ggatgttgcc aaaaagatcc tgcgtcttcc cgcccaaaca gggtttcccg
     1441 cggacctcgg gggtatcgtt gacaaagtgg atgagccggg atttgtgacg gcggtgggac
     1501 ttgccttgtg gggactggag gatatgccgg tgaggtcaac gagcatgaaa atgccaggac
     1561 tcaataatat gccgaatatg gggaaaacgg tcgagaaaat gaagggatgg ttccggaagt
     1621 ttctgcccta gaagtgtgat ttctcagcca gaggctgatc cgcctttggc ggaaaatcca
     1681 aaatcccaag tccaaatgtc aaacaaaaat caaaatccca aattttctgc tagttgacga
     1741 taaccttgat ttttgacatt aggatttggg attttattgg aaattgagat ttggtaattg
     1801 gaaattaata aatcagggag acttcttgac attttttgag gctatatgct agattgacat
     1861 accagtttat tagtcaatat cacaaacatt atggccgaag taaaaccaga aattgaaaca
     1921 tttgcccgta taaaagtggt tggagtcgga ggttcgggag gaaacgcgat tagtcgaatg
     1981 attgaccaca agctccgggg agtggaattc gtggcgatca acacggacgc gcaggatctt
     2041 catcattcca aagcggcgga aaaaattcat atcggaaaaa atctgaccaa ggggcttgga
     2101 gccggaatga atccggagat cggtcgccag gcggcggaag aaaatcggga cgaaattcac
     2161 gagattttga aaggggcgga tatggttttc gttacctgcg gtatgggagg cggaaccgga
     2221 acaggcgctg ctccaattgt tgccgaagcg gccaaagaag ccggcgcgct gaccattgcc
     2281 gtcgtcacca aacctttcag cttcgaggga gcgcaaagaa aagcgatcgc ggcggaagga
     2341 cttgaaaact tgagagaaag agttgatgcg ctgatcacaa tttcaaacga caggcttctt
     2401 cagataatcg accggaaaac tacccttatc aacgctttcc gtgttgtgga tgatgttctt
     2461 cggcaaggtg tccagggaat ttcagacctc atttcaaaac caggaatcgt caatgttgac
     2521 tttgccgacg tgaaagccat cttgcaggat tccggttcgg ctctgatggg aatcgggacg
     2581 gcgtcgggtg aaaaccgcag tgcggaagcg gccaagttgg ccatcaacag tcctcttctt
     2641 gaattctcaa ttgacggcgc aaagggcatc ctgtttaatg tgagcggcgg accggatatg
     2701 actatgctcg aaatcaatga agcggcaaac gtgatcactg aaagcatcga tccgaatgcc
     2761 aaagttatct ttggtgcatc ggttgatgaa tcggcgaaga agggcgagct tacgatcacg
     2821 gttgtggcag ccggtttcga cgctgaaatg gtaaatgaac ccttgtcctc aatgcagcct
     2881 gtttcaatgt cgcgagaaac aaaacctgcc attgaagagc ctatgatttc ttcttcatct
     2941 tcattttcag acgaggaaaa gcccaagttt atcattgaag aaaaaatggc tcccaagccg
     3001 aaaagacaga ttaacttcga gtcaaaaacc agcacgattg gcgaagagga agatgaagag
     3061 ctggagatcc cggctttcat ccggagaaag atgaagaagt aggatcgacc tgtggataac
     3121 tcaaaatctt tcaataaaaa agcccgcttg acgggctttt tgcttgtggt aaaataagga
     3181 ttactaccac aacatatagt atgaaaaggg gattctgcga attctcctta ttatttctct
     3241 caattttatg ctctgtccct tttgcaatca tcccgaaaca aaagtgattg attctcgcga
     3301 atcaactgat ggaaaggtga tccgccgccg tcgggaatgt ttgaaatgcc agaagcgctt
     3361 cagcacgtat gaacaattgg aactcctgaa ctttgtagtc gtgaaaaaag atggacgcag
     3421 ggaagactac aaaagggaaa aactcgaaaa gggaatcagg aaagcgctcg aaaaaagacc
     3481 gatcgacgga aaaaaaatcg aagaagctat tgatgagatc gaatacaaac ttcagctcga
     3541 caagaattg
//
LOCUS       DATKGS010000012         3612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_43853, whole genome shotgun sequence.
ACCESSION   DATKGS010000012 DATKGS010000000
VERSION     DATKGS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43853"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VMQ48_00245"
     CDS             complement(<1..548)
                     /locus_tag="VMQ48_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08513.1"
                     /translation="MEIIRNVWQKNKYLQNTTIIHSYDGEKSWYSEKYLEDELVTIKN
                     SWHFQGASDLIDAGIKKFKKDIDYVIVLASDTWLINPAYIERILIRMEKEALYLATNP
                     WGLLKRNDLRDVGMAVDFFIVDAKWAKKYKMFPVDYGEFHKKYEDLFLYQNGGNVMLE
                     KLIWARFIKAVGREQEFKGSATP"
     gene            complement(680..750)
                     /locus_tag="VMQ48_00250"
     tRNA            complement(680..750)
                     /locus_tag="VMQ48_00250"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Trp,seq:cca)
     gene            complement(755..1411)
                     /locus_tag="VMQ48_00255"
     CDS             complement(755..1411)
                     /locus_tag="VMQ48_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08514.1"
                     /translation="MKRKEIFSKANRLREHGRSFREISEILGIAKSTASLWTRATVLN
                     PRAIQRLERLQDLGRKKSKKTKERQKMLIEKEIAEKAKMTVRNAKLNDSHRKIICSLI
                     YWCEGGKNDGGRVNFTNSDPQLMRYFLKIFRKSFSLSENKFRALLHIHEYHQEKKQIR
                     FWSKVTKIPIKQFSKSFHKKNTGKNTRAGYPGCLSVRYYDSKIQKELVFICKEIFKQR
                     "
     gene            complement(1478..1633)
                     /gene="rpmG"
                     /locus_tag="VMQ48_00260"
     CDS             complement(1478..1633)
                     /gene="rpmG"
                     /locus_tag="VMQ48_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HUD08515.1"
                     /translation="MSQDNLIKMKCEVCKSVNYHSTRNKKKIKEKIELKKYCRKCRKH
                     TLHKESK"
     gene            complement(1666..2514)
                     /locus_tag="VMQ48_00265"
     CDS             complement(1666..2514)
                     /locus_tag="VMQ48_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08516.1"
                     /translation="MGYRSWKNKKNKNAPSQLHPSVTTTQPSSSDSAKTDSNTSSDQS
                     DQADQDFEAQCQNGEWVKIADVQGDISSATGILHWVDMDNDAASKQFENYTHYLDGKE
                     KIGLVNPAAKSADNANNLDLFQGREVEVQGVAKQGVAREMQVSQVKCTGSETDKNLAT
                     SRVSMLSYISENISSIAPEKAPYQKWSVDSAIILDEKDVYVDYYDTLQDDANSDPNLD
                     TMHRVLLQVSPKDGGSYDVKVLAYYVPGEEDFSLKQGTDKFKNQDETAFPSYTYDSEE
                     NSWTRD"
     gene            complement(2601..2673)
                     /locus_tag="VMQ48_00270"
     tRNA            complement(2601..2673)
                     /locus_tag="VMQ48_00270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2638..2640),aa:Thr,seq:ggt)
     gene            2828..3487
                     /locus_tag="VMQ48_00275"
     CDS             2828..3487
                     /locus_tag="VMQ48_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUD08517.1"
                     /translation="MELLNKRNTGLIISVAFIGFLVWSTVEFQLYFSKILTLFQNLTQ
                     RNEGLSILIFIGLSAAMTMISSFIRVLPIPIALILWGNTPALLYLLVGWLIGDALSYA
                     IGYSAGNPIIKKFVALDKLDYYREKIPPRAQFKLILFFVTAMPAEIPNYVLGSIRYDF
                     AKYFIATIFSELIYVYLSVRTGQALVQQNFVIFAIAVTLLVALFSYSFHLFNIYLKEK
                     K"
BASE COUNT          912 a          823 c          658 g         1219 t
ORIGIN      
        1 ggggtagccg aacccttaaa ttcttgctcc ctgccaacag cttttatgaa acgagcccat
       61 attagcttct ctagcatcac atttccacca ttttgataaa gaaaaagatc ttcgtatttt
      121 ttatgaaatt caccataatc aactggaaac attttatatt ttttcgccca ctttgcatca
      181 acgataaaaa aatcaacggc cattcctacg tctctaaggt cattcctttt tagcaagccc
      241 caagggttgg ttgcaagata taatgcttct ttttccattc ttatcaagat acgttctata
      301 tacgctggat ttatcaacca ggtatctgaa gccaatacga ttacgtaatc aatatctttt
      361 ttaaactttt ttatacccgc atcaattaag tccgaggctc cctggaaatg ccaagagttt
      421 ttgatagtta ctaattcatc ttcaagatat ttttccgaat accagctctt ttctccatcg
      481 taagaatgaa tgattgttgt attttgaagg tacttatttt tctgccaaac atttctgata
      541 atttccatgt ttatctttgc gtcgtctact ctattgtatg tgtacatcaa aactcctatt
      601 tttttcatcc tatttctctc actttttatg aatttgtcct gtcgcgagaa agtttgtagc
      661 agttcttttt tatactatgc agggatggag ggaatcgaac ccccggttcg gcttttggag
      721 agccgtggtt taccactaac caacatcccc aatcttatcg ttgtttaaat atttctttgc
      781 aaataaaaac taattccttt tgaattttgc tgtcatagta cctaacactc aagcatcccg
      841 gatagccagc ccttgtattt ttacccgtgt tttttttatg aaacgattta gaaaattgct
      901 tgattggaat ttttgtgact ttcgaccaaa atcgaatttg ttttttctct tgatgatatt
      961 catgtatatg aagtaaggca cgaaatttat tttcacttag agagaaagat tttctgaata
     1021 ttttcagaaa atacctcatg agctgaggat ccgaattggt aaaattcacc cttcctccat
     1081 cattttttcc tccttcacac cagtatatca atgaacatat tattttgcga tggctatcat
     1141 tcagcttggc atttctcacc gtcatttttg ctttctccgc aatttctttt tctatcagca
     1201 ttttttgtct ttcttttgtt ttctttgatt tttttctgcc taaatcctga agcctttcca
     1261 gcctctggat cgctctggga ttcaaaacag tcgcccgagt ccacaagctg gcggtactct
     1321 tggctatacc aagaatttcg ctaatctctc gaaaagagcg tccatgctct cttaatctat
     1381 ttgcttttga aaatatctct ttgcgtttca tatactaaaa ctacgggtta tgaaacctat
     1441 aatcatagta cgaatatttc tttcaaaagt caaatgtcta cttcgactct ttgtgcagcg
     1501 tatgcttccg gcatttgcgg caatattttt tgagctcaat tttttccttg atctttttct
     1561 tgttgcgggt tgaatgatag ttcactgact tgcaaacctc gcatttcatt ttgatgagat
     1621 tgtcttgaga catatttttc cgataaaact tataaattat atatcctagt ctctggtcca
     1681 ggaattttcc tccgagtcat atgtataaga aggaaaggcg gtttcgtcct gatttttgaa
     1741 tttgtccgtc ccctgcttca gggaaaaatc ttcttcaccc ggaacatagt aggccaaaac
     1801 tttcacgtcg tagcttccgc cgtcttttgg cgaaacttgg agcaggaccc tgtgcatcgt
     1861 atcaagattt ggatcgctgt ttgcatcatc ctgcagagta tcatagtagt caacgtaaac
     1921 atctttttcg tccaatatga ttgccgaatc caccgaccat ttttgatagg gtgctttttc
     1981 aggcgcaatt gagctgatgt tctcggaaat atagctaagc atggaaacgc ggctcgtggc
     2041 aagatttttg tccgtttctg atccggtgca cttcacttgg gaaacctgca tttcacgggc
     2101 cacaccttgc ttcgcaacgc cctggacttc aacttcccgg ccttggaaca aatcgaggtt
     2161 gttcgcattg tcagctgact ttgcagccgg attcacaagc ccaatttttt cttttccatc
     2221 cagatagtgg gtataatttt cgaattgctt cgaggccgca tcattgtcca tatcaaccca
     2281 gtgcaaaatt ccggtggcgg acgagatatc gccctggaca tcggcgattt tcacccactc
     2341 gccgttttgg cattgggcct caaaatcctg atcggcttgg tcggactgat cgctgcttgt
     2401 gttgctatct gttttcgcag aatcacttga agacggctgt gttgtcgtta cagacggatg
     2461 cagctgcgaa ggcgcattct tatttttttt gttcttccag ctccgatatc ccaaaaaccc
     2521 ggcagcaacc aagatcagta caacaagaaa tatttatatt tttttcccgg acatattaat
     2581 aagaataaat attatccttg agccgttgcg cggattcgga ccgcggaccc cttccttacc
     2641 atggaagtgc tctgccaact gagctacaac ggcctatata gcaggaatga caaagcttag
     2701 cggataagga aatatagtcc gcagcatcgc ttcgacttga accatattat caaaaaacgc
     2761 ataaaaaaac aacccgccga aaaaagaagg ctttctgcgg ctggtttttt gttgtatact
     2821 gcgatcaatg gaactcctca acaaaagaaa caccggtctg atcatctcag ttgctttcat
     2881 cggctttttg gtctggtcaa ctgttgagtt tcagctctat ttttcaaaaa tcctcacgct
     2941 ttttcaaaac ctcacccaga gaaacgaggg tttgagcatt ctgatcttca taggcctttc
     3001 ggcggccatg acaatgattt cttctttcat ccgggtcctc ccaatcccca ttgccctcat
     3061 attgtggggc aatacgccgg ctctgctcta tctgctcgtg ggatggctca tcggcgacgc
     3121 tttgagctat gcaatcggct attcagccgg aaatccgatc atcaaaaaat ttgttgcgct
     3181 tgataaactg gactattatc gcgaaaaaat tccgccgcgc gcccaattca agctgatcct
     3241 tttttttgtc actgcgatgc cggcggaaat tccaaattat gttcttggct caattcgcta
     3301 tgatttcgca aaatatttca tcgccacaat tttttccgaa ctgatttatg tttatttgtc
     3361 cgttcgcacc gggcaagctt tggtgcagca aaattttgtc atttttgcga ttgccgtgac
     3421 tctcttggtt gcccttttca gctattcctt tcatcttttc aatatatatc taaaagagaa
     3481 aaaatagccg gaataaatgc ctactttatg cataaaaaaa ggcgacctca ttgagatcgc
     3541 cctctccgcg aatagtgaaa cgcgggatta ttcatcgttg tccatatgca gcgagagact
     3601 tgctgttatt tt
//
LOCUS       DATKGS010000013         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_72555, whole genome shotgun sequence.
ACCESSION   DATKGS010000013 DATKGS010000000
VERSION     DATKGS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72555"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VMQ48_00280"
     CDS             complement(<1..435)
                     /locus_tag="VMQ48_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HUD08518.1"
                     /translation="MRILASLILAMIVASAIPANAAGASVDSNQDAKQTLETKITNTL
                     SDNFLVALYTPKVGNVQFSKMYKNKLLSENFILSKGSFVVNASAYTAAADECGKSDGI
                     TASGKKVQANHTLACPKQYQLGTKIEIDGMGTYVCEDRGGAIR"
     gene            complement(583..750)
                     /locus_tag="VMQ48_00285"
     CDS             complement(583..750)
                     /locus_tag="VMQ48_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08519.1"
                     /translation="MQKFELIKQLEYLIAFQTNCLASGDWESFDNAQDKIKKLEEAII
                     SRNEEGQVNTA"
     gene            955..1290
                     /gene="rplS"
                     /locus_tag="VMQ48_00290"
                     /pseudo
     CDS             955..1290
                     /gene="rplS"
                     /locus_tag="VMQ48_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            1436..1519
                     /locus_tag="VMQ48_00295"
     tRNA            1436..1519
                     /locus_tag="VMQ48_00295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1470..1472,aa:Leu,seq:gag)
     gene            1539..1611
                     /locus_tag="VMQ48_00300"
     tRNA            1539..1611
                     /locus_tag="VMQ48_00300"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1572..1574,aa:Val,seq:tac)
     gene            1626..1697
                     /locus_tag="VMQ48_00305"
     tRNA            1626..1697
                     /locus_tag="VMQ48_00305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1658..1660,aa:Phe,seq:gaa)
     gene            1709..1779
                     /locus_tag="VMQ48_00310"
     tRNA            1709..1779
                     /locus_tag="VMQ48_00310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1741..1743,aa:Gly,seq:gcc)
     gene            complement(1796..2497)
                     /locus_tag="VMQ48_00315"
     CDS             complement(1796..2497)
                     /locus_tag="VMQ48_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HUD08520.1"
                     /translation="MIKIIFENKDIIVVDKPAGLAVHPGVGTSEKTLVDFLLEEFPEI
                     KNVGDDPEVRPGIVHRLDKETSGVMVVARNQKTFEFLKDLFKNRQIEKKYLALVHGKL
                     KTKEGQVEGEMGRSKKDFRKQALVRGKISVRKERYSLTHFKVKKEFEKYSLLEVFPKT
                     GRMHQIRVHLHSVGHPIVGDKKYTFKEFKNIFAPRMMLHANSISFIGPDKEKYFFESR
                     PPEEFEKFMDHKSAR"
     gene            complement(2500..2583)
                     /locus_tag="VMQ48_00320"
     tRNA            complement(2500..2583)
                     /locus_tag="VMQ48_00320"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2547..2549),aa:Leu,seq:tag)
     gene            2738..3670
                     /locus_tag="VMQ48_00325"
     CDS             2738..3670
                     /locus_tag="VMQ48_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUD08521.1"
                     /translation="MNIPLMIIGIVVIYLSAVIRYVPPPFEWVIEENFPGSDTVKKIW
                     KPGLHLLWFPIKPFMFVRNKLYCADKKILITIGVADENPGDASLVEFKDTSAGVRTQA
                     ILKVLDPIKATYMVDDYEKAALNKIEADFRRIMSTMKLDDAMSNICMRTQIAAETFKN
                     VNKAIARWGVKLTNPGDEITIIDFVLQQSTLDDRAKILTAQKDHDAKIKNAEATRRET
                     ILIKEGEAKGIILVADAEGKGEADKVTILAKKLGLPNEQVIDYLLKLGMLKAIDGSTI
                     IATSEGGLLNTPIGLAQTMFAINNAQAGKGGSQP"
     gene            3667..>3871
                     /locus_tag="VMQ48_00330"
     CDS             3667..>3871
                     /locus_tag="VMQ48_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08522.1"
                     /translation="MIYGAYIGLAIVVCSVLKEWFSWPQTILIGTGVTLVILVVWAFR
                     SQFTKIIFPASAVSPPTSTTTGSG"
BASE COUNT         1090 a          859 c          877 g         1045 t
ORIGIN      
        1 tctgatcgcg ccaccacggt cctcgcagac ataggttccc atcccgtcaa tttcaatttt
       61 cgttccgagt tgatattgct tgggacaagc aagagtatga tttgcctgaa cctttttgcc
      121 gctagccgtg attccgtctg atttgccgca ttcatcggcc gccgcggtat aggccgaagc
      181 attcacaacg aagcttccct tcgacaatat gaagttttcc gagagcagct tgtttttgta
      241 catttttgag aattgcacgt ttccgacttt cggcgtatag agcgcaacca gaaagttgtc
      301 actcaaggta tttgtaattt ttgtttctaa tgtttgtttg gcgtcctggt tcgaatcaac
      361 actcgctcca gccgcattcg caggaatggc cgaagcaaca atcatagcga gaataagcga
      421 agccaaaata cgcatcagat tgaataaatt gttactgtca ggcttttact tcaggcaaca
      481 aaaaaagccc ttttcaaggc ctaacgggct agtatagcat tttattatgc ggaagtcaag
      541 ggcacgtgaa acaatgacta tttctcgtga aacatatttt ttttaggccg tattcacttg
      601 gccttcttca ttcctactta tgatggcttc ttccagcttt tttattttgt cttgggcgtt
      661 atcgaagctt tcccagtctc cgcttgccag acaattcgtc tgaaaagcga tcaaatattc
      721 cagttgtttg atgagttcga acttttgcat agtctgaaaa ataaaaaata cttcgcgaaa
      781 aagtaatttt gattcccttt tttgcccctc ctgattttag gcactatagt agtatagtcg
      841 ataattttct ttttgtcaac agcttgacga ttttagaaaa aaattatagt atgaattagc
      901 tttccaaaag ccagtttttg tttttggagc aataacccta atatttctaa taaaatggaa
      961 aaggttcaga aattcaacac aaaggttcgc acgtctttgg aaaggccgga tctcaaagca
     1021 ggggatatag tcaaaattca ccgtaagatc aaggaaggag aaaaagagag agtccaggtt
     1081 tttgagggta tcgtcattgg tgtaaaaggg aaacaaagct cttcgcccac ggccactgtg
     1141 aggagggtat cttttggaac aggcgtggaa atcaccgtcc ctcttcattc acccgccata
     1201 tccaagatcg aggttgtgaa acaggccaag gtgcgccaag caaaactata ttatcttcga
     1261 ggcaaaggaa ttcgcatttc aaaactgaaa acaaaagagc tcgcaaagtt tgtggcagag
     1321 gaaaaagcgg aagagccaaa ggccgtaatt gaaccagcta agcagacaga agaaaaatca
     1381 ggggagaatg ctgaggacaa aaaatagatt ttgatattga gcgtcattct cagatgccgt
     1441 ggtgccggaa tcggtagacg ggccagcttg aggggctggt gtccattagg gcgtgggagt
     1501 tcgagtctcc ctcacggcac tgttctagct gtagcgacgg acgattagct cagctggtag
     1561 agcgcttgct ttacaagcaa gatgtcgcag gttcgagccc tgcatcgtcc acaggaatca
     1621 aaaaagccgg gttagctcag tggtagagca gaggactgaa aatccttgtg tgcccagttc
     1681 gattctggga cccggcacaa tatggcaggc gggtatagta caatggtagt acattaggtt
     1741 gccaatctaa aaacacgggt tcgattcccg ttacccgctc aaaagcaaaa gcgccctacc
     1801 gggcgctttt gtgatccatg aatttttcaa attcttccgg cggccgactt tcgaagaaat
     1861 atttttcttt gtccggcccg atgaagctga tcgagttggc atgaagcatc atccgcggcg
     1921 cgaaaatatt tttaaactct ttgaaagtat actttttgtc tccgacaatc ggatggccga
     1981 cggaatgaag atgaacccgg atctgatgca tccgaccggt ctttggaaaa acttcgagta
     2041 gggaatattt ttcaaattct ttcttgacct tgaaatgagt cagggaatat ctttcttttc
     2101 tcacggatat tttcccgcgc acgagcgctt gctttcgaaa atcttttttc gaccggccca
     2161 tctcgccctc aacctggcct tctttcgttt tcaacttgcc gtgaaccaac gccagatatt
     2221 ttttttcgat ctggcgattt ttgaacaagt ccttgagaaa ctcaaaagtt ttttggtttc
     2281 ttgccaccac catcacgccc gaggtttctt tgtcgaggcg atggacgatc cccggacgca
     2341 cctccggatc atcgccgaca tttttgattt caggaaattc ttccagcaaa aaatcaacaa
     2401 gcgttttttc acttgttccc actcccggat gaacagccaa tccggccggc ttgtccacga
     2461 cgataatgtc cttgttttcg aagataatct tgatcattgt gccggggaga ggacttgaac
     2521 ctccatagga ttgctcccgc cagctcctaa ggctggtgcg tctgccaatt tcgccacccc
     2581 ggcgtataaa tataatagca acttgggtaa aatagcacac attgacaagc ttgtaaaata
     2641 gtgatataat gacccgatgc tcttggagtg tcaaagaagg gaaattaagc cctctttggg
     2701 ggctctttaa aaaatttgat gtatacgagg tgaaaaaatg aatattcccc tgatgattat
     2761 tggcatcgtt gtgatttatc tttctgcagt tattcgttac gtcccccctc cttttgaatg
     2821 ggttatcgag gaaaattttc ctggcagcga cacagtaaag aaaatttgga aaccgggtct
     2881 tcatttacta tggtttccaa ttaaaccctt catgtttgtg agaaataaat tgtactgcgc
     2941 ggacaaaaag atcctgataa ccataggcgt cgctgacgaa aatccaggcg atgcgagcct
     3001 agtagagttt aaggatactt cggcgggcgt aagaactcaa gccatcctaa aggttctcga
     3061 ccccataaag gcgacttaca tggtcgacga ttacgaaaag gcagccctca ataaaatcga
     3121 agctgacttt cgcagaatca tgagcactat gaaattggac gacgccatga gtaatatctg
     3181 catgagaacg caaatcgcag ctgaaacgtt taaaaacgta aacaaggcca ttgccagatg
     3241 gggagtaaaa ttaaccaacc caggcgatga aataactatc attgatttcg tcctgcagca
     3301 atcgactctg gacgatcgcg ccaagatcct aacagcccaa aaggatcatg acgcaaaaat
     3361 taaaaacgcg gaagcaaccc ggcgagaaac aatcctaata aaagaaggcg aggcaaaggg
     3421 cataattctt gtcgctgacg ccgagggaaa aggtgaggcc gataaagtaa caatcctcgc
     3481 caagaaactt ggtcttccga atgaacaagt gattgactat ctacttaaac tggggatgct
     3541 caaggcaatt gatggctcga caatcattgc tacaagcgag ggaggattgc taaacacccc
     3601 gataggtctc gcacaaacaa tgtttgccat caataacgcg caggcaggaa aaggaggaag
     3661 ccagccatga tctacggtgc ttatattgga cttgcgatcg ttgtgtgcag cgttttgaaa
     3721 gaatggtttt cctggcccca gacaatttta attggcaccg gtgtaactct ggtaatcctt
     3781 gttgtgtggg cgtttaggag tcaatttact aagatcattt tccctgcttc tgccgtatct
     3841 cctccaactt caacaacaac cggttctggc a
//
LOCUS       DATKGS010000014         3388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_14859, whole genome shotgun sequence.
ACCESSION   DATKGS010000014 DATKGS010000000
VERSION     DATKGS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14859"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VMQ48_00335"
     CDS             complement(<1..1088)
                     /locus_tag="VMQ48_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08523.1"
                     /translation="MKSWSVKLGKGISAMKRDGILRGGKRSVVSFFKLFGRVGSGDIL
                     FISSGVGDSAKYRSERQAEELRLHGFKCSVTVQDNPLLPRYADRFKIFIFQKTIYSNT
                     VAKMIENIKAQKKEIIFETDDLVFDLKYVKQMDFYQKLNSLEKKQYETGVGSEILRDD
                     YVKTCVTTTSYLADILRGYGKQVFISTNKLSNYDVGIMNDIFEKNEKYDGEKIKIGYF
                     SGTASHDKDFETVKDPLVYILAKHPQVELHLFGPLEIDYVYDKFGHRVKRHPFAPWEK
                     YLENISSVDINIAPLEIGNPFCESKSAIKFTGSGLLGIPTVAAATQTFREAIEDGVDG
                     FVAGDDGEWKEKLDKLILNGDLRRQMGE"
     gene            complement(1085..2242)
                     /locus_tag="VMQ48_00340"
     CDS             complement(1085..2242)
                     /locus_tag="VMQ48_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08524.1"
                     /translation="MMKVLTIAATSFFSDRGCHMRIYGDAKYLEKFGAEVKICTYFSG
                     DDIAGLDIERIGKVGWYKRTAPGFSWGKFWLDIKLIFLCRRVIREFRPDIIHGHMYEG
                     FGVGYVAKRLTFQNPPVVVDLQADLEEEFKSYNRKNFIARRFFVWLSRRLINRCNWLV
                     VSSENVKPRMEKLYKHKDKISIVRDGIDLDLFRNVLRLTEEEQAKIAEIKKWTEGKKL
                     LVYIGGLSDNKGVGDLLEAFLRLNLKNAEWRLLIGGFGNDEEKYKEYVRKNNLGDFVY
                     FAGRVKYFSLPAYLALASAAIDPKNGSTTEGSGKLVNLMAAGCPIVCFEKEFNRARLG
                     EKGHYMQNFGDLAGILESIKTSEKIHYDMEELGEEKEAKKLFDIFEKLIKK"
     gene            complement(2239..2706)
                     /locus_tag="VMQ48_00345"
     CDS             complement(2239..2706)
                     /locus_tag="VMQ48_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HUD08525.1"
                     /translation="MNIKDRIDALVEKILGRKVNKSLLQYFRYLMCGAVATATDVGIL
                     FVLTHFLHLNYLVVAAASFLCGITVNYILNSFLVFQSSGKIKREFSVFAMIGLGGLAW
                     TELILWVLVDKLKIYVMIAKLVAIILVLQWNFFMRKRFVFYPEPIPGDNIEKI"
     gene            complement(2719..>3388)
                     /locus_tag="VMQ48_00350"
     CDS             complement(2719..>3388)
                     /locus_tag="VMQ48_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08526.1"
                     /translation="FFRKKELKRDTITAVYIIIFIFIFYLGNAVNSVIATVRYQIIVY
                     PLIFVVAAIGVSQFFEMEKVKKFVPLLTAYAVLIIVLLGSLFIARPNFLAYASEILPN
                     NFIVNLKGMGEGSFEAASYLNSLPNAHDMTIWSDKGAVCEVFAGKCFIDFKKTTFLQN
                     KIDYFVVSTDRRSRSLKMSKSLKNIADFEKIYKSNNYSFRFIINNNPNDFVKVFKSSE
                     FLSQ"
BASE COUNT          921 a          852 c          632 g          983 t
ORIGIN      
        1 atctcgccca tttgtctcct caaatctcca ttcagaatca atttatccaa tttttctttc
       61 cactctccat catctccggc cacaaatccg tccacgccat cttcgatcgc ttctctgaat
      121 gtttgagtgg ctgcagcgac ggtgggtata cctaaaagtc ctgatccagt gaacttgatt
      181 gccgatttcg attcacaaaa aggattgccg atttcgagcg gcgcaatatt gatatcaacg
      241 ctagagatat tttcgagata tttttcccag ggcgcgaaag gatgtctttt cacccgatgg
      301 ccaaatttat catatacata atcaatttca agcggtccaa aaaggtgcaa ttctacttgc
      361 gggtgtttag cgagaatgta cacaagggga tctttcacgg tttcaaagtc cttgtcatga
      421 ctggctgttc cggaaaaata accaattttt attttttctc catcatactt ctcatttttt
      481 tcaaaaatat cattcatgat tccaacatca taattcgaaa gtttatttgt tgaaataaaa
      541 acttgcttgc catatcccct caatatatcg gcgagatacg aagttgttgt cacacaggtt
      601 tttacataat catctcttag aatttcactg ccaactccgg tttcgtactg tttcttttct
      661 aaagaattta atttctgata aaaatccatc tgcttcacat atttcagatc aaaaaccaaa
      721 tcgtccgttt caaaaataat ttcttttttc tgcgctttga tgttttcgat catcttcgcc
      781 acggtgttgg aatagatcgt cttttggaaa ataaaaatct tgaaccggtc agcgtagcgc
      841 ggcaaaagtg gattgtcctg aaccgtgacg ctgcatttga acccatgcag cctcaactct
      901 tccgcctgcc gctcgctgcg atatttcgcg ctatccccca ctccgctcga aataaaaaga
      961 atatcccctg atccgacccg cccgaacagc ttgaaaaaag aaacgacgga tctttttccg
     1021 ccacgcaaaa tcccgtcgcg tttcatcgcg ctgatcccct ttcccaattt gaccgaccac
     1081 gatttcattt ttttatcagc ttctcaaata tatcaaacaa ctttttggct tctttttcct
     1141 cacccaattc ttccatatca taatgaattt tctctgaagt cttgatactt tccaaaatgc
     1201 cggccaaatc gccgaaattc tgcatataat ggcctttttc tcccaatctc gcacggttga
     1261 attctttttc gaaacaaaca atcgggcatc cggcggccat caaattgacc aatttcccgc
     1321 tgccttctgt tgtgcttccg tttttcggat caatcgcggc cgaagccagt gccagatagg
     1381 ccggcaagga aaaatatttg actctccccg caaaatatac gaaatctcca agattatttt
     1441 ttctgacata ttctttgtat ttttcttcat cgtttccgaa cccgccaatg agcaacctcc
     1501 attctgcatt tttaaggttt agccttaaaa atgcttcgag caagtctccg acgcctttgt
     1561 tgtcgctcaa gccgccgata tagaccaaca attttttgcc ttccgtccat tttttgatct
     1621 ctgcaatctt tgcctgctct tcttccgtca agcgcagaac attccggaaa agatccaaat
     1681 caatcccatc gcggacgatg ctgattttgt ctttatgctt gtataatttt tccatgcgcg
     1741 gcttcacgtt ctcgctgctc accaccagcc aattgcaacg gttgatcaaa cggcgggaaa
     1801 gccatacgaa aaatcgtcgg gcgatgaaat ttttccggtt atagcttttg aactcttctt
     1861 cgagatccgc ctgcaaatca acgacaacgg gaggattttg aaatgtcagt ctttttgcca
     1921 catacccgac accaaatcct tcgtacatat ggccatggat aatatccggt cgaaattccc
     1981 tgatcacccg gcggcacaaa aaaatcaatt tgatgtcgag ccaaaatttc ccccaggaaa
     2041 atcccggggc cgtccgtttg taccatccga ctttcccgat ccgttcaata tcaagccctg
     2101 caatatcatc ccccgaaaaa taagtgcaaa ttttcacttc agctccgaat ttttcaagat
     2161 atttcgcgtc gccgtatatc cgcatatggc agccgcgatc agaaaagaaa gacgtcgccg
     2221 caatcgtcaa aactttcatc atattttttc gatattatcc ccgggaatcg gttccggata
     2281 gaaaacaaat ctttttctca tgaaaaaatt ccactggaga accagtatga ttgccaccaa
     2341 cttggcgatc atcacgtata ttttcagctt gtccaccaaa acccaaagga tcagctccgt
     2401 ccaggccagg cccccgaggc caatcatcgc gaaaacggaa aattctcttt tgattttccc
     2461 gctgctctgg aaaaccaaaa acgagttgag gatatagttg acggttatgc cgcaaagaaa
     2521 gctggccgcc gccacgacga gatagttgag atgcaaaaaa tgggtcaaaa caaagaggat
     2581 gccgacgtcc gtagctgttg cgaccgctcc gcacatcaga tagcgaaaat attgcaaaag
     2641 actcttgttg acctttcggc caaggatttt ttcaacaagc gcgtcgatcc gatcttttat
     2701 gttcataaat atctcatgct attggcttag aaattcggac gatttgaaaa ctttcacaaa
     2761 atcgttcggg ttgttgttga tgatgaatcg gaaggaataa ttgttgctct tgtatatctt
     2821 ctcaaagtcc gcgatatttt tgagcgattt ggacatcttg aggctgcggg accggcggtc
     2881 ggtcgaaacg acaaaatagt cgatcttgtt ctgcaaaaat gtcgtttttt tgaaatcgat
     2941 gaaacatttt ccggcaaaaa cctcgcaaac cgcccctttg tccgaccaga tcgtcatatc
     3001 atgggcattg ggcaggctgt tgagatagct ggccgcctca aagctccctt cgcccatccc
     3061 cttgaggttc acgatgaaat tgttcggcag aatttcggac gcataagcca gaaaatttgg
     3121 ccgggcgatg aacaggctgc ccaaaagcac aatgatcaag accgcgtatg ccgtcaaaag
     3181 cggaacaaat ttcttgactt tttccatttc aaagaattga gaaacgccga tggccgccac
     3241 cacaaaaatc agcgggtaca ctatgatctg gtatctcacc gtcgcaatca ccgagtttac
     3301 cgcgttgccg agataaaaga taaagataaa aatgatgata tataccgctg tgattgtatc
     3361 gcgcttcagc tcttttttcc tgaaaaaa
//
LOCUS       DATKGS010000015         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_202283, whole genome shotgun sequence.
ACCESSION   DATKGS010000015 DATKGS010000000
VERSION     DATKGS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202283"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..229)
                     /locus_tag="VMQ48_00355"
     CDS             complement(74..229)
                     /locus_tag="VMQ48_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUD08527.1"
                     /translation="MNLFRSNGFEEFQALGDVSFEVKKGEFFGIIGRNGSGKSTLLKI
                     LAGAKCP"
     gene            complement(371..1066)
                     /locus_tag="VMQ48_00360"
     CDS             complement(371..1066)
                     /locus_tag="VMQ48_00360"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HUD08528.1"
                     /translation="MALDISVHKSILLQILKDIYSDADISPYLGFKGGTAAYIFFDLD
                     RFSVDLDFDLLDEAKEEQVFSRIEKIVKNYGQIKEARKKRFNLFFLISYEEKAQNIKV
                     EINRRMFGSQYEMKTYLGISMLVMVKEDMFAHKLMAMHERMGKSNRDIFDVWFFSKNH
                     WPINKKIVEERSRMSFAELLKKCIVQLEKTTSRNILDGMGELLSEKQKDWAKANLVSD
                     TIFLLKLMRENEK"
     gene            complement(1071..1652)
                     /locus_tag="VMQ48_00365"
     CDS             complement(1071..1652)
                     /locus_tag="VMQ48_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HUD08529.1"
                     /translation="MDKGQYLSSILRCGKTVFSFSDIAMIWGESGSAARVRLNYYVKG
                     GELFRIRRGIYAKDKNYDKLELASKIFTPAYVSFETVLGREGVTFQHYGQIFAASYLT
                     REILCDNQKYSFRKMKKTILSDSAGVENKNNRAIAVKERAFLDVLYINKDYHFDNLSP
                     LDWNRVFEILPIYDNKRMDKKVKEIHKNFISQK"
     gene            complement(1755..>2661)
                     /locus_tag="VMQ48_00370"
     CDS             complement(1755..>2661)
                     /locus_tag="VMQ48_00370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUD08530.1"
                     /translation="LAKKLMDRGDKVVIIDNFNDYYDPQLKRDRIKILLKGYKFKLYR
                     GDIRDAKLVEKIFKTEKIDKVIHLAAMAGVRNSLRDPKLYFDVNVMGSLNLLEAAVKY
                     KIKNFVFASSSSVYGNNKKIPFSESDSVDTPISPYAASKKCTELMAHVYSHIHKLPTT
                     GLRFFTVYGPWGRPDMALFLFTDAITKGQPIKVFNRGKMSRNFTYVDDIATGTITVLD
                     KAKDYGVMNIGGDKEETLMRYIEVLEENLGKKAKKKLLPMQPGDVPKTVADIKKLRKL
                     GWKPTTRIEKGIKNFVDWYKEYYKN"
BASE COUNT          666 a          593 c          537 g          865 t
ORIGIN      
        1 ggggattgtg aatttgaatg tttttctgat tacagtatac ttacgataat cctactctta
       61 agtatactct ttgtcaagga cattttgccc ccgccaaaat cttgagcagc gttgattttc
      121 cgctgccgtt gcggccgata atgccgaaaa attccccttt tttcacttca aagctgacgt
      181 cgcccagcgc ctggaattct tcgaatccgt tcgagcggaa caggttcaca aaagcccccc
      241 cgcaagctgg agatcttttc gtggggaatg cggaaggttt tggagacgtt ttccaccttg
      301 atggccgttt cggccggctt ggggttttca gattttgttg aagtttcgtt atccattctt
      361 gtattttatc ttatttttca ttttcccgca tcagcttcaa aagaaaaatc gtatcagaca
      421 caagattggc tttcgcccaa tctttttgtt tttcgctcaa caattctccc ataccgtcca
      481 aaatattgcg actggtcgtt ttttccaatt ggacaatgca ttttttcaac aattccgcga
      541 aagacattct ggatctttcc tcgactattt ttttgtttat cggccagtga ttttttgaaa
      601 aaaaccagac atcaaaaata tcgcgattgg attttcccat acgttcatgc atcgccatga
      661 gcttgtgggc aaacatatcc tctttgacca taaccagcat tgagatgcca agataagttt
      721 tcatttcata ttgagaacca aacatccggc gattgatttc aaccttgata ttttgagctt
      781 tttcctcata tgaaatcaga aagaacagat tgaatctttt ttttcttgct tcttttattt
      841 gtccgtaatt tttaacaatt ttctctattc tggaaaaaac ttgctcctcc tttgcttcgt
      901 caagcaggtc aaaatccaga tcgacggaaa aacgatccaa atcaaaaaaa atatatgcgg
      961 ctgtgccgcc cttgaagcca agatagggag aaatatcggc atcagaatag atatctttca
     1021 atatctgaag cagaatactt ttgtgaactg atatgtccag cgccataaaa ttatttttgg
     1081 gatatgaaat ttttgtggat ctcttttact tttttgtcca ttcttttgtt gtcgtatatc
     1141 ggcaatatct caaaaaccct gttccaatcc aggggcgaca aattgtcaaa atgatagtct
     1201 ttgttgatat acaaaacatc aagaaaagcc ctctctttca cggcaatcgc gcgattgttt
     1261 ttgttttcca cgccggcaga atcgctcaat atggtttttt tcattttgcg gaaagaatat
     1321 ttttgattgt cgcacaaaat ttctcttgtc agatatgaag ccgcaaaaat ttggccgtaa
     1381 tgctggaagg tgacgccctc tcttcccaga acagtttcaa aactgacata agctggagtg
     1441 aatatttttg aagccagttc aagtttgtca taatttttgt ccttggcata tattccgcgg
     1501 cgaatacgaa aaagttctcc gcctttgaca taataattca gcctgactct ggcagcagac
     1561 cctgactcgc cccaaatcat agcaatatca gaaaaagaaa atactgtttt cccgcagcgc
     1621 aatattgatg aaagatattg acctttgtcc atattgagaa cccgactata gtttaaacct
     1681 tgtatgcagt tcctattgta ccaaatatgc tctttttgtc aaattttgat aaattccgaa
     1741 aaactcgtct tttttcagtt tttataatac tctttatacc aatccacaaa atttttgatc
     1801 cccttttcga tccgggtcgt tggtttccag ccaagtttcc gcaatttttt gatatccgcc
     1861 accgttttgg gaacatcgcc cggctgcatg ggcaggagtt ttttcttggc cttttttccg
     1921 agattttctt ccagaacttc gatatatctc atcagcgttt cttccttgtc gccaccgata
     1981 ttcatgacgc cgtagtcctt tgctttgtcg agcacggtga tcgtgccggt cgcgatgtcg
     2041 tccacatagg tgaaattgcg gctcattttt ccgcgattga aaactttgat cggctgacct
     2101 ttggtgatgg cgtccgtgaa aagaaaaaga gccatatccg gccgtcccca cgggccatag
     2161 acagtgaaga atcgcagtcc cgtcgtcggg agcttgtgga tgtggctata gacgtgcgcc
     2221 atcagttcgg tgcatttttt gctcgccgca tagggcgaaa tgggcgtgtc aacgctgtcg
     2281 gactcggaaa acggaatttt tttgttgttg ccgtagacgg atgacgacga agcgaaaacg
     2341 aaatttttga tcttgtattt caccgccgcc tcgagaaggt tgaggctccc catcacgttg
     2401 acgtcgaaat acagtttcgg gtcgcgaaga gaattgcgaa ccccggccat ggcggcgagg
     2461 tggatcactt tgtcgatttt ttccgttttg aaaatttttt cgacgagctt cgcgtcgcgg
     2521 atatcgcccc tgtataattt gaatttgtac cctttgagca aaattttgat ccggtcgcgc
     2581 ttgagctgcg gatcatagta atcattgaag ttgtcgatga tgaccacttt gtctcccctg
     2641 tccatcagtt ttttggccag a
//
LOCUS       DATKGS010000016         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_44280, whole genome shotgun sequence.
ACCESSION   DATKGS010000016 DATKGS010000000
VERSION     DATKGS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44280"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="frr"
                     /locus_tag="VMQ48_00375"
     CDS             <1..436
                     /gene="frr"
                     /locus_tag="VMQ48_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HUD08531.1"
                     /translation="VEYYGAKTPLKQMASINVPEPRLIVVQPWDKDSLVNIEKAINEA
                     QLGFNPVNDGQVIRLAIPPLNEERRTELVKTLGKYAEYAKVEVRKAREVAWDEIQDMV
                     KDGKLAEDDKFRGKDMLQKSVDEYNGKIEEIREKKEKEMMEI"
     gene            617..1777
                     /gene="rseP"
                     /locus_tag="VMQ48_00380"
     CDS             617..1777
                     /gene="rseP"
                     /locus_tag="VMQ48_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HUD08532.1"
                     /translation="MFTVLVFIVILGLLVFVHELGHFLVARRNGVMAEEFGFGFPPRI
                     FGLYKTKNGKRKLVWGSKEVESTDTIYSVNWIPLGGFVKIMGEDGDEKKDPKSFASKP
                     AFTKTRILAAGVAMNFLLAAVLLSVGYFVGIPQAVDDAASGNLKNEKIQIIETIPGSP
                     AEQMGIQVGDQIIGAVEGNNQVVRFNTIEDVQNFINAKKGQEIKLDVKRGNETLALSG
                     TPRTDFPASEGALGISLVKTAIVSYPWYESIWRGVQTTVDLAGMIIVAFGNLFWKLIT
                     GHSVGMDVSGPVGIAVLTGQVAKLGFIYILQFTALLSVNLAIINILPIPALDGGRILF
                     ILIEKIKGKAVSQKFEARAHNIGFALLITLMVVVTFRDVARLDVLQKLKNLF"
     gene            1980..>2181
                     /locus_tag="VMQ48_00385"
     CDS             1980..>2181
                     /locus_tag="VMQ48_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUD08533.1"
                     /translation="MFSRIFGRFSKNVGIDLGTANTLVYVEGRGIVINEPSVVAVNQK
                     TGQILAIGAEAKKMVGKTPAHPP"
BASE COUNT          627 a          410 c          560 g          584 t
ORIGIN      
        1 ggttgaatac tatggcgcga aaactccttt gaaacagatg gcgagtatca atgttcctga
       61 gccgcggctc atcgttgttc agccgtggga caaggacagt cttgtgaaca ttgaaaaggc
      121 gatcaatgag gctcagttgg gattcaatcc ggtgaacgat ggccaagtga tccgtctggc
      181 cataccgcct ctcaatgaag agcgtcgcac ggagctcgtg aaaactctcg gaaaatatgc
      241 agaatatgcc aaggtagagg tgcgaaaggc gcgcgaagtt gcttgggatg agatccagga
      301 tatggtcaag gatggaaaat tggcagaaga tgacaaattt cgcggaaagg atatgctcca
      361 gaagagcgta gatgaataca atggaaagat cgaagaaatc cgggaaaaaa aagaaaaaga
      421 gatgatggaa atctgacgga aattttcaat tttcaattta caaattttca aacaattttc
      481 aatgacccaa tgattcaatt tcaaaaacaa aatcatttag tcatttagaa atttagacat
      541 tgtttagtaa ttgaaaattt agtaattgaa aattcggttg ggaaattaga gttgaaagat
      601 tgtttgtata aaaagtatgt ttacagtatt agtctttatt gtcatccttg gtcttcttgt
      661 tttcgtccac gaactgggac attttttggt tgcgcggcga aatggagtga tggcggaaga
      721 gtttggattc ggttttcccc cgaggatttt tggcttatat aaaacgaaaa atggcaagag
      781 aaaactggtt tggggatcaa aggaagtgga atcgaccgac acgatatatt ccgtcaactg
      841 gatacctctt ggcggtttcg tgaagatcat gggcgaagac ggggacgaaa aaaaagatcc
      901 aaaaagtttt gcttcgaagc cggcgtttac caaaacaaga atcttggcgg caggcgtggc
      961 catgaatttt ttgctcgcgg cggtgctttt gtcggttgga tattttgtcg gcataccgca
     1021 ggcagtggat gatgcggcga gcgggaatct caaaaacgaa aaaatccaga tcatcgaaac
     1081 aattcccggt tcgccggccg aacaaatggg tattcaggtc ggcgatcaga tcattggtgc
     1141 ggttgaaggg aataatcagg ttgtccgctt caatacaatt gaagatgttc agaattttat
     1201 caacgccaaa aaaggtcagg aaattaaatt ggacgtgaag aggggaaacg agacactcgc
     1261 gctctcggga accccacgga ccgattttcc cgccagcgaa ggcgcgctcg ggatcagcct
     1321 ggtgaagacc gccatagttt cctatccgtg gtatgaatca atttggcggg gagtacagac
     1381 gacagttgat ctcgccggga tgatcattgt ggcgtttgga aatttgtttt ggaaactcat
     1441 caccggtcat tcagtgggga tggatgtttc cggtccggtc ggcatcgcgg tcttgacggg
     1501 acaagtggcc aaactcggct tcatctatat tttgcaattc acggctcttc tttcggtcaa
     1561 cttggccatc ataaatatcc tccccattcc ggcgcttgac ggcggacgaa tcttgtttat
     1621 actgatcgaa aagatcaagg gcaaagctgt ttcgcaaaaa ttcgaagcgc gcgcccacaa
     1681 cattgggttt gcgctcctga taacactgat ggtcgttgtg acctttcggg acgtggcgag
     1741 actggatgtc cttcaaaaat tgaaaaattt attctaggag ttattagtat gaggattatc
     1801 tacgcgcttt aatgcgcctt tttttattgg ctaagcgaac tttgacataa tatcattact
     1861 gaagtaatat taggtactag gaataggaaa ataaatataa aaatggcttg aaaattcaca
     1921 catcgctgaa gcgttttttt ggatttgtct aattttttca tttcgtgttt cgctaaagta
     1981 tgttttcaag aatcttcggg agattttcaa agaatgtcgg gattgacctt ggaacggcca
     2041 atacccttgt ctatgttgaa ggtcgcggga tcgtcatcaa tgaaccgtcg gttgtggcgg
     2101 tgaaccaaaa aacggggcag attttggcga ttggcgcgga ggccaaaaaa atggttggca
     2161 aaacacccgc tcatcctccc c
//
LOCUS       DATKGS010000017         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_115270, whole genome shotgun sequence.
ACCESSION   DATKGS010000017 DATKGS010000000
VERSION     DATKGS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115270"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VMQ48_00390"
     CDS             complement(<1..496)
                     /locus_tag="VMQ48_00390"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HUD08534.1"
                     /translation="MSDKKENMMETIVSLCKRRGFIFPACEIYGGFANAYSFGPYGAE
                     LKNNIKRLWWKKFVHAREDIVGIDGPILLHPKLWEASGHVAGFNDAMIDCKKCKKRFR
                     ADHLVEELTGEDMEGRLEDMTNTLRNLKAKCPNCGESEWTDARNFNMMFETKMNGFDG
                     TVYLR"
     gene            complement(738..1469)
                     /locus_tag="VMQ48_00395"
     CDS             complement(738..1469)
                     /locus_tag="VMQ48_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08535.1"
                     /translation="MSSRIEKLRNLFLFVFFLLVISIIFTPLLVQRGFSIFPEESLES
                     VLLFFQVFIGWQVFRLYEQSVSHREKEIKKLEGEYQKREKELLEAFAYLGKVNVQISL
                     IRSFLQKIKAPSSRKEAESYINEILSIALTLSGKKWATLRAIDAKSLQTVKEYWAKTN
                     PEIDTGGIKISNKDVSSWGKEGDKCDRKGYCVYASAGSKLSPFKVFLVFLNGEKMDQD
                     IEGFLRAAANQCEVLLTMFDFKEKR"
     gene            complement(1574..>2240)
                     /locus_tag="VMQ48_00400"
     CDS             complement(1574..>2240)
                     /locus_tag="VMQ48_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08536.1"
                     /translation="MPRKTKSKSARVAGGKAIIKKTKTSTVSMNHHRKDMRFKVSLVI
                     LIGMLTFSAMLLLLSLNKSSAVNAETGTITAIPASHNFGVKKSSSQTLNDSALDFTVT
                     VPVELGAWFYKIGEVKSLTDKSLSDQYFRVYVANPGVKSNNFDEQNKDILTIRKFSAD
                     EWSAVEKSCQKNKSDICDAAGTLIVKSDKEAYAYTKPVDCPKSIVAKCNLTDKIIESF
                     NLK"
BASE COUNT          572 a          556 c          416 g          696 t
ORIGIN      
        1 ggcgcaagta taccgtccca tcaaaaccat tcattttcgt ttcaaacatc atgttgaaat
       61 ttcgggcatc agtccactct gactctccgc agttcggaca tttggccttg agattccgaa
      121 gtgtattcgt catatcttcc aatcgacctt ccatatcttc tccagttagc tcctcgacca
      181 agtggtcagc gcgaaatctt tttttgcatt ttttacaatc tatcatagca tcattgaatc
      241 ccgctacatg gcccgacgct tcccaaagtt tcggatggag cagaattggc ccatcaatgc
      301 caacaatgtc ttcgcgggca tgcacgaatt ttttccacca aagccgcttg atattgtttt
      361 tcagctctgc tccatatgga ccgaaagaat aggcatttgc aaatccgcca taaatttcac
      421 aagctgggaa tatgaacccc cgccgtttgc acaacgacac gattgtctcc atcatgtttt
      481 cttttttatc gctcataata tttcttaaat tattatatta aattaaacaa aaaacccgtc
      541 ctaaatatcc aaaaaacaaa cttcagatat tcgggacgag ccttctttcg aattcccgcg
      601 gttccacccg agttccccat cgctggatgc aatgaggctc tttgatttat tgagttgtca
      661 ccacttgagg ttgccattcc tcacagatta tatactaatc aatttcagtc tttctgtcaa
      721 caacaaaaac acaaaactta tcttttctcc ttgaaatcaa acatagtaag cagcacttcg
      781 cattgattgg ccgccgcccg caaaaaacct tcgatatcct gatccatttt ttctccattt
      841 aggaacacca gaaaaacctt gaagggcgaa agttttgatc ctgcggaggc ataaacacaa
      901 tatccttttc tgtcgcattt atccccttcc ttcccccaag aagaaacgtc cttgttgctg
      961 atcttaatgc cgccggtatc gatctccggg ttggttttcg cccaatattc tttgaccgtt
     1021 tggagcgatt tggcatcaat cgcccggagt gtcgcccatt ttttcccact gagggtcagg
     1081 gcgatgctca aaatctcgtt tatatagctc tcggcctcct tgcggctcga gggggctttg
     1141 atcttttgga gaaacgaccg gataagcgaa atttggacat tcacttttcc gagataggca
     1201 aacgcttcca acaattcttt ttctcttttt tggtattccc cttccagctt ttttatttct
     1261 ttttcgcggt ggctcacgga ttgttcataa agccggaata cctgccaacc gatgaagacc
     1321 tggaaaaaaa gcaaaactga ctccaggctt tcctcgggaa aaatcgaaaa gcccctttgc
     1381 accaaaagcg gagtgaaaat tatcgaaata accagcagaa aaaatacgaa caaaaacaaa
     1441 tttctcagct tttctattct tgaactcata gtttgtgccc tacacccccg aatttatgtt
     1501 ccctattaat tacgattttt ttcgatatat atttcatctt tcaaaaaagg ccccccgtcg
     1561 ggaggcccct tgattatttc aggttgaaac tttcaattat tttgtccgtg agattgcatt
     1621 tggccacgat gcttttcgga caatccactg gtttcgtgta agcgtaggcc tctttgtcac
     1681 ttttgactat cagtgttccc gccgcatcgc aaatatcgct tttgtttttc tggcaacttt
     1741 tttcaacagc ggaccattca tcggcgctga attttctgat cgtcagaatg tctttgtttt
     1801 gctcgtcaaa attgtttgat ttcactcccg gattcgcaac atataccctg aaatattgat
     1861 cggacaggga tttgtccgtc aagcttttca cttcgccgat cttataaaac cacgcaccca
     1921 gttcgaccgg aaccgtgaca gtgaaatcaa gcgcgctgtc attgagtgtc tgactgcttg
     1981 attttttgac gccgaaatta tggcttgccg gaattgccgt gattgttccc gtctccgcgt
     2041 tcactgccga agatttattc aaagaaagaa gcagcagcat cgcagaaaaa gtcagcatcc
     2101 cgatgagaat cacaagggaa actttgaagc gcatatcttt ccgatggtga ttcattgaaa
     2161 ccgtgctggt ttttgttttt ttgataattg ctttcccgcc agcaacgcgg gcagactttg
     2221 attttgtttt tctaggcata
//
LOCUS       DATKGS010000018         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_202499, whole genome shotgun sequence.
ACCESSION   DATKGS010000018 DATKGS010000000
VERSION     DATKGS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202499"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VMQ48_00405"
     CDS             complement(<1..473)
                     /locus_tag="VMQ48_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08537.1"
                     /translation="MITDEVKIKLRAGRGGDGVVAFDKTKMSLGPTGGRGGNGGNVYF
                     EGVSDITALNKYKTNREYFAEDGKRGKGDRSSGHEGKDLILTVPIGSVLRDLKTGKEF
                     EITKHGERILTAKGGLGGRGNFFFRSSRNTTPKEFELGKPGEEFEFAVELRSPSSP"
     gene            586..1014
                     /locus_tag="VMQ48_00410"
     CDS             586..1014
                     /locus_tag="VMQ48_00410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HUD08538.1"
                     /translation="MPINSKNEKEKIKYFVKRSRAGLGLFAVDFFKKGEFVIEYVGER
                     LVGKKADDRYNKYLFEVNSQLTIDGSGRENMARYVNHSCRPNCEVRIYAQRVRIWTIR
                     KIKPGEQFSYDYGKEYFDEYIKPVGCKCASCRKKRKKQKI"
     gene            1103..2014
                     /locus_tag="VMQ48_00415"
     CDS             1103..2014
                     /locus_tag="VMQ48_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUD08539.1"
                     /translation="MANNNYGLGRGLASLIPPKNKPAASAGEPAKNVSQMPAKIQSEA
                     LQDDFLEVAIEKVASNPQQPRHNFDEKELADLAASIKEHGIIQPLVVAKIAPEQYELI
                     AGERRLKASKLAGLTMVPVIIREESGEREKLELALVENIQRHDLDVLEEARAYKKLIE
                     EFDLTQEEVADKVGKSRSAVANKVRLLGLPIEIQRALSEGKISEGHARSILAIENTEK
                     QRALFEMILKDNLTVRQTEEKVKEVTVATHQRKIGSSISPFQIEEEALAAKFGTKVSI
                     KKAGNGGKITIDFYSKEELDNILSKIS"
     gene            complement(2066..>2593)
                     /locus_tag="VMQ48_00420"
     CDS             complement(2066..>2593)
                     /locus_tag="VMQ48_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HUD08540.1"
                     /translation="EIQIRTPRMHNEAEFGIAAHWIYSSAKTWRDYFMRRYSPEEIPE
                     QELFWVKQLHEWQTETGRDDEDFMKSIKVDFFQNHIFAFTPKGDIINLPEGATPIDFA
                     YAVHSEIGARTTGAKVDGKLVALDYKVRNGQVIEILTSKEMKLPNQDWLRIVSTTYAK
                     SRIRQQLRKAGIKTR"
BASE COUNT          680 a          591 c          641 g          681 t
ORIGIN      
        1 ggggaggagg gagatctgag ttcgaccgca aattcaaatt cttctcccgg ttttccaagt
       61 tcaaattctt tcggggtcgt gtttcgggac gagcggaaaa agaaattacc ccgtccgccg
      121 aggccgcctt tggctgtcag gatcctttcg ccatgtttcg taatctcgaa ttcttttccg
      181 gttttgaggt cgcgaagaac cgagccgatc gggacggtta aaatcagatc tttgccctcg
      241 tgtccgctcg agcggtcgcc ttttccgcgt tttccgtctt cggcaaaata ttcccggttg
      301 gtcttgtatt tgttgagcgc ggtgatgtcg gaaacgcctt caaaatatac atttccgccg
      361 tttccgcccc gtccgccggt tggcccgagg ctcatctttg tcttgtcaaa agcgacgacg
      421 ccatcgccgc cacgccccgc ccggagtttt attttcactt catctgtgat catgcatgca
      481 gcttagcttt ttgcgaggat tttggcaaca aaatctccag atcttgaatt ttttttattt
      541 ttattctatg attatatttg tggtcaaaaa gcgatatttt tatttatgcc aatcaattca
      601 aaaaatgaaa aagagaaaat aaaatatttt gtgaagagat cccgtgccgg acttggtctt
      661 tttgccgtcg attttttcaa aaaaggcgaa ttcgtgatcg agtatgtcgg cgagcggctg
      721 gtggggaaga aggccgatga tcgctacaac aaatatcttt tcgaagtcaa ttcgcagttg
      781 acgattgacg gatcgggtcg agaaaatatg gctcgctatg tcaatcattc ctgccggccg
      841 aattgcgaag tgaggatcta tgcccagcgc gtccggatct ggaccatccg gaaaatcaaa
      901 ccgggggaac agttttccta tgactacgga aaggaatatt ttgacgaata catcaagcct
      961 gtcggctgca aatgcgcgag ctgccggaaa aaaaggaaaa aacaaaaaat ttaaacttcc
     1021 ggcctcaaaa gggctggttt ttctattcct ccattttttg gtataataaa cccagcaata
     1081 aattttctta aaaccaaaac aaatggcaaa caataactat ggacttggcc gaggactagc
     1141 ttctcttata cccccgaaaa ataaacccgc cgcttcggcg ggcgagccag caaaaaatgt
     1201 ttcacagatg ccggcgaaaa ttcagtcgga agcgcttcag gacgattttc tcgaggtggc
     1261 gattgaaaaa gtcgcttcca atcctcagca gccgcggcac aatttcgacg agaaagagct
     1321 ggcggatctg gccgcgtcca tcaaagaaca cgggattatt caacctcttg tcgtggcgaa
     1381 aatcgcgccc gagcaatatg aactgatcgc cggggagcgt cgcctcaagg cttcgaagct
     1441 ggccggactt acgatggttc cggtgatcat ccgtgaagaa tcgggagagc gggaaaaatt
     1501 ggaactggct ctggtggaaa atatccagcg acatgacctc gatgtacttg aggaagcacg
     1561 ggcgtacaaa aaattgatcg aagaattcga ccttacccag gaagaagtgg cggataaggt
     1621 cggaaaatcc cgcagcgcgg tcgccaacaa agtgcgtctt ctgggactcc ccattgaaat
     1681 tcagcgtgct ctttcggaag gaaagatcag tgaaggacac gcccgttcga ttcttgcgat
     1741 tgaaaatacg gaaaagcaaa gagcgctttt tgaaatgatc ttgaaagaca atttgaccgt
     1801 tcgccagacg gaagaaaaag tgaaagaagt gacggtggcg acgcatcaaa gaaaaatcgg
     1861 ctcaagcatt tcgccgtttc aaattgaaga agaagcactc gccgcaaaat ttgggacgaa
     1921 agtgagcatc aaaaaagcgg gcaacggtgg aaaaatcaca attgactttt attcaaaaga
     1981 agaactcgat aatattttga gcaaaatttc ttagatattc aatatttctt ccgtagagtc
     2041 gaggcattgc ctcgcctcta cttttttacc tggtttttat tcctgctttt cggagttgct
     2101 gcctgattcg cgatttggca taggtcgtgc tgacaatgcg cagccagtct tggttcggaa
     2161 gtttcatttc tttggaagtg aggatttcaa tcacttggcc gttgcgaact ttataatcaa
     2221 gtgccaccag ttttccatcc acttttgcgc cggttgtgcg ggccccgatt tcgctgtgaa
     2281 cggcgtaggc aaaatcaatc ggcgtcgcgc cttcgggaag attgatgatg tcgcctttcg
     2341 gagtgaaagc gaaaatgtgg ttttggaaaa aatcgacttt gatgctcttc atgaaatcct
     2401 catcatctct tccggtttct gtctgccatt cgtgaagctg tttcacccag aacaattcct
     2461 gctcggggat ttcttcgggc gaatagcggc gcataaaata gtcacgccac gttttggccg
     2521 aagaatatat ccagtgggcg gcaattccga actcggcttc gttgtgcatc cgcggcgtgc
     2581 ggatctggat ttc
//
LOCUS       DATKGS010000019         5093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_2378, whole genome shotgun sequence.
ACCESSION   DATKGS010000019 DATKGS010000000
VERSION     DATKGS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..747)
                     /locus_tag="VMQ48_00425"
     CDS             complement(340..747)
                     /locus_tag="VMQ48_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08541.1"
                     /translation="MQKKANKSNAVKYAVIGASLAGLAATAYFFFGPKGKKRREHAKA
                     WAIKMKGEVVEKLEKAREITEPIYREIIDSVARDYKKGKKASQPEIAALAADLKKHWK
                     TMSKMAIAAKQEVAKNASRVAKKVAKKSNRRKA"
     gene            complement(768..1028)
                     /locus_tag="VMQ48_00430"
     CDS             complement(768..1028)
                     /locus_tag="VMQ48_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08542.1"
                     /translation="MDTLVKADIFFFISSIATIVIAVLLSIVLFYFIKAGKNLYQISQ
                     KLQEHFKESEEFVLELKERLEDNMVFRLFFPPARVRSRDKKK"
     gene            complement(1074..1391)
                     /locus_tag="VMQ48_00435"
     CDS             complement(1074..1391)
                     /locus_tag="VMQ48_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HUD08543.1"
                     /translation="MFGIVRTLVSLVVGVIEFLLLLRFIFKFFVANLGTPFVAWIYST
                     TASLVGPFAKIVPDLKLGQFVVDFSTLVALIVYVFIGYLILRAFSYVGPRYYNRDDGE
                     YRD"
     gene            complement(1409..1804)
                     /locus_tag="VMQ48_00440"
     CDS             complement(1409..1804)
                     /locus_tag="VMQ48_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08544.1"
                     /translation="MTEIEKETITTPENGGNQVVSEPIAQKATGSQTTEYLVYYIFGA
                     LEIILAFRFVLKLAGASLGNAFVGFVYGLSGIFVAPFAGIFHRGFTQGVETTSVFEPS
                     TVIAILVYAVVAWGIVKLVRISSGEKQAS"
     gene            complement(1801..1980)
                     /locus_tag="VMQ48_00445"
     CDS             complement(1801..1980)
                     /locus_tag="VMQ48_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08545.1"
                     /translation="MTIDKKQRVTLFLDPNLLKQAKVQALVENESLSALIERALTKYL
                     PKEIIIKKEKGGEKT"
     gene            2297..2680
                     /locus_tag="VMQ48_00450"
     CDS             2297..2680
                     /locus_tag="VMQ48_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUD08546.1"
                     /translation="MKTSKSLKKFGKLLYDLRIKEEITLREMCRKVNYDPSNWSKIER
                     GKIAPPSERKTLEIWARTLGLKKDSREFDNFIYNANVAQSIIPLEIMQEKELVAALPA
                     FFRTLKNKKPTKGDIDKIIDLIKNA"
     gene            2698..3306
                     /locus_tag="VMQ48_00455"
     CDS             2698..3306
                     /locus_tag="VMQ48_00455"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HUD08547.1"
                     /translation="MKYKNIAVPFVGNSYIKNKADEFRLKFWGKDIPVEIEEIVEIKL
                     KIKVIPIPDLMSQCGVDAQITSDFTSIYVDQKNYENDTNRFRFSLAHEVGHYVLHEKF
                     YKDLKISSFADVIAFMDEIDAEEYSHLEVQANKFGNYLLLPRKELLRIRNTVLEEIRK
                     KHDIKNIDEKTLNSYLAGYISSQFVVSSGATEIALNDLNNSK"
     gene            3360..3530
                     /locus_tag="VMQ48_00460"
     CDS             3360..3530
                     /locus_tag="VMQ48_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08548.1"
                     /translation="MPEQKNIHKLKKSSAYSFSVTIPKEIVEKYKWREKQKLVVKDKG
                     HGKVEIIDWRKK"
     gene            3704..4021
                     /locus_tag="VMQ48_00465"
     CDS             3704..4021
                     /locus_tag="VMQ48_00465"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HUD08549.1"
                     /translation="MGKYKAKIPEIKEKIVKEINPEKVILFGSYAWGTPTDDSDVDLL
                     IIQKSDEPRRTRQINLRRKLFGSGVAMDFIIYTPDELDRRLSIRDVFVNKILRQGKVL
                     YER"
     gene            4011..4409
                     /locus_tag="VMQ48_00470"
     CDS             4011..4409
                     /locus_tag="VMQ48_00470"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HUD08550.1"
                     /translation="MNDKNIEQFQEWQKRAEEDFGSAKMLLDNDGFPSVVCFHAHQVI
                     EKYLKGYLAYQDKEPQKTHQLDILLEECVKMDESFRGYIEEAVSLNDYYIEARYPTDF
                     REDIPLSEAKEALEKAEKIRDAVVSKIQSN"
     gene            4520..>5093
                     /locus_tag="VMQ48_00475"
     CDS             4520..>5093
                     /locus_tag="VMQ48_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUD08551.1"
                     /translation="MANDDVAVRFNKVSFEYGHKKPILDEVSFALRRGTKFTIMGQNG
                     SGKSTILNLITGALKETDGSIYVDPRLKIAYAKQVIPREQMQFTVKEFFEQYFDGKTY
                     NIDPKIDGALKLVNLEAKHDKKIKSFSGGQQSRLLLASALIQDPDLLILDEPTNNLDA
                     AGIASLTEFLMEYKKTCIVISHDANFLNCFT"
BASE COUNT         1581 a          990 c         1090 g         1432 t
ORIGIN      
        1 aaaacactcg atagcatcgc cactccctgt tcagtgaaag catacggatt agttcgtgaa
       61 aatttgatat gtttgaggtg gtcacagttt gtgaccacat aatctttttc cgctttcgta
      121 agttcaaaca taaaatcttc cggaaatctt ttatgagcag aacaatattt cgtatttcaa
      181 agaattgttt ttgaagcagt tcgaatttgc ggtggaaaat tattttggat aagattagct
      241 gctcctgaag ttgaacttca ggaatttcca agtcaaaaaa cccagccccg acgtgacgtc
      301 gggactgttt ttgaacgcgt aaaaaatgta tttcaatacc taggcttttc ggcgattgga
      361 ttttttggca acttttttgg cgacacgaga ggcatttttg gccacttctt gcttggcggc
      421 aattgccatt ttgctcatcg ttttccagtg cttcttgagg tcagccgcca aggccgcaat
      481 ttccggctgg ctggccttct ttcctttttt gtagtctctg gccactgagt cgatgatctc
      541 tcggtagatc ggttccgtga tttcccgcgc tttttcaagc ttttccacaa cttctccctt
      601 cattttgata gcccaggctt tcgcgtgctc acggcgcttc ttgcctttcg gcccgaagaa
      661 gaaataggct gttgcggcga gacccgcgag acttgctcca atcaccgcgt acttcactgc
      721 atttgatttg tttgcttttt tttgcatgat atatagttat aaaaaaatta ttttttcttg
      781 tcccggctac ggacgcgcgc cggcgggaag aacagccgga aaaccatatt gtcctcaagc
      841 ctttctttga gctcaagcac gaattcttcc gactctttga aatgttcttg cagtttctga
      901 gaaatctgat agagattttt gcccgctttg atgaaataaa aaaggacaat ggaaagcagc
      961 acggcgatca ctatggttgc aatggaagat ataaaaaaga aaatgtctgc ctttactaat
     1021 gtatccatat gcataagtat actacaccga taccttttcg cataggctat ttatcaatcc
     1081 cgatattccc cgtcatccct gttataatat ctcggaccga cgtaggaaaa ggctcgcaag
     1141 atcaggtagc cgatgaagac gtatacgata agcgcgacca gcgtggaaaa atcgaccaca
     1201 aactgtccca atttcagatc aggaacgatt ttcgcgaaag ggcctaccaa agaagcggtg
     1261 gtgctataga tccaagccac aaacggagtg cccaagttgg caacgaagaa cttgaaaatg
     1321 aatcggagca gcagcaaaaa ttcaatcact ccgaccacaa ggctcaccaa tgttctgaca
     1381 atgccgaaca tatatttgat ttaattgatt aggaagcttg tttttcgccc gaggagattc
     1441 gcaccagttt gacgattccc caagccacta cggcgtaaac aagaatggcg atcacggtcg
     1501 agggttcgaa aacggaagtt gtttccactc cctgagtgaa tcctcggtga aatattcccg
     1561 cgaaaggcgc gacaaaaatt ccggacaggc catagacgaa acccacaaag gcgtttccca
     1621 agctggctcc cgccaatttg agcacaaaac ggaaagccaa gatgatttcc agcgctccga
     1681 agatatagta gaccagatat tcagttgtct gtgagccggt cgctttttgc gcgatcggtt
     1741 cactcacaac ctgatttccg ccattttccg gggtggtgat tgtttctttt tcaatttcag
     1801 tcatgttttt tcacctcctt tttctttttt gattattatt tctttgggca aatatttggt
     1861 caaagctctc tctatcaaag cggacaagct ttcattctca accaaagctt gcactttggc
     1921 ctgtttcagc agatttggat caagaaagag ggttacgcgc tgttttttgt cgattgtcat
     1981 ggtgcatatc ttacaagtag ttgtataggt tgtacttatt actacgctgg agacagctct
     2041 ttatttcatc gaagctggag aatccgggct tgcctgaaaa aacaggagtg ataggattaa
     2101 aagagggaat agaagcttct ttgatgttcc ctagggttaa actttaggaa tgactaaaaa
     2161 aaagaggata aaataagctt aattaataaa tttcccatct tgcaaaggat tgtttttttg
     2221 aaatatttgc tggttcttga cagtattatc tagtgtggta atattatacc acagtataaa
     2281 tattaaaata tatcatatga aaacgagtaa aagcttgaaa aaattcggga agctcctgta
     2341 tgatcttcgg attaaggaag aaatcactct tcgggaaatg tgccgcaagg taaattatga
     2401 tccgagcaat tggagcaaga tcgagagagg aaaaattgca cctccttctg aaagaaaaac
     2461 tttggaaata tgggctcgaa cgcttgggtt gaaaaaagac tccagagaat ttgataattt
     2521 tatctacaat gccaatgttg ctcaaagcat aattccgctt gaaatcatgc aagagaaaga
     2581 attggtggcg gctttgccgg ctttttttag gacgctgaaa aataagaagc ctactaaggg
     2641 agatatcgac aaaataatcg atttaatcaa aaatgcctag gcataacttg catagaaatg
     2701 aaatacaaaa atatagctgt tccatttgtt gggaattcat atataaaaaa caaagccgac
     2761 gaattcaggc tgaaattttg gggtaaggat atcccagttg aaattgaaga aatagtcgag
     2821 attaagttaa aaattaaagt aatacctata cccgacctga tgtctcaatg tggagtagat
     2881 gcgcagataa cctctgattt tacgtcgatt tatgtcgatc agaaaaatta tgaaaacgac
     2941 acgaaccgtt tcaggttctc cctagctcat gaggttgggc attatgtttt gcatgaaaaa
     3001 ttttataaag acttgaagat ttctagtttt gcggatgtca ttgcttttat ggatgaaatt
     3061 gatgcagaag aatattctca tttggaagtt caggccaaca aattcggaaa ctacttattg
     3121 ttgccaagga aagaattatt aagaatcaga aatactgttc tggaagaaat tcgcaaaaaa
     3181 catgacatca aaaatattga tgaaaaaaca ttgaactcat atttggccgg atatatttct
     3241 tctcagtttg tggtttcttc cggtgcgact gaaattgctt tgaatgatct aaataattca
     3301 aagtgaatta ttggcagagg cttctatgaa ggcttctaat aaaaattaat ataaaaccta
     3361 tgcccgagca aaaaaacatc cacaaactca aaaaatccag cgcgtatagt ttttccgtga
     3421 ctatccccaa ggaaatcgtc gaaaaatata aatggcgcga gaagcagaaa ctggttgtca
     3481 aagacaaggg tcacggcaaa gtcgaaatca tcgattggcg aaagaaataa aatagaaact
     3541 tctatgtaat caaatgatca aaattttcgc cgcaatcgtg ggaataggga tcattttcac
     3601 cgggtattta ttcaatcttg tcctaaaata acacaatttt tacgaaccat ttctgacaac
     3661 cgcaaaattt gctgtttatt gcaatttaag tataattaag gcaatgggta aatataaagc
     3721 aaaaattcct gaaatcaagg aaaaaatagt caaggaaatc aatccggaga aggtgattct
     3781 tttcggctct tacgcgtggg gaactcccac cgacgatagc gatgtggatt tgctgataat
     3841 tcaaaagtct gacgaacctc gcagaacaag acaaatcaat ttgagaagaa aactttttgg
     3901 aagcggggtg gcaatggatt ttattatcta tacgcctgac gagcttgata ggcgattgag
     3961 cattcgagat gttttcgtga ataagatttt gagacaagga aaggttttat atgaacgata
     4021 agaatattga acaattccaa gaatggcaaa aaagagcaga agaagatttt ggttcggcca
     4081 aaatgcttct cgacaacgat ggtttcccgt cggttgtatg ttttcatgcc catcaagtga
     4141 ttgagaaata tctcaaggga tatttggcct atcaagataa ggagccgcaa aaaactcacc
     4201 agctggatat tctacttgaa gagtgcgtca aaatggacga atctttcagg ggctatatcg
     4261 aagaagcggt ttccttgaac gattattaca tagaagccag atacccgact gatttcaggg
     4321 aggatatacc tttaagcgaa gcgaaagagg ctcttgaaaa ggccgagaaa atacgggatg
     4381 ccgttgtatc gaaaattcaa agcaattagt attcgccaaa gtcaaaattt agtagaaccg
     4441 tcttcattga cggttttgcc ttttcaaatc cgcaaaagcg atgtataatc agaattcagt
     4501 gtttaaccaa gaaaaaacaa tggcgaatga tgatgtcgcg gtgaggttca acaaagtttc
     4561 ttttgaatac ggccacaaaa agcccatttt ggatgaggtc tcttttgcgc tgcggcgggg
     4621 gacgaagttc acaatcatgg ggcaaaacgg ctcggggaaa agcacgatct tgaatttgat
     4681 caccggcgcg ctcaaggaga ccgacggttc gatctatgtc gatccgcggc tcaagatcgc
     4741 ctatgcaaag caagtcattc cgcgcgagca aatgcagttc acggtcaaag agtttttcga
     4801 gcaatatttt gatggaaaaa catataatat cgatccgaaa attgacggcg cgttgaagtt
     4861 ggtgaatctt gaagccaaac acgacaaaaa aataaaatcg ttttccggcg gacaacagtc
     4921 gcggcttctt ttggcttcgg cgctcatcca agatccggac cttctcattt tagacgaacc
     4981 gaccaacaac ttggacgcag ctgggatagc aagtctcacg gaatttttga tggagtacaa
     5041 aaaaacttgt atcgtcattt cccacgatgc caattttctc aattgcttta ccc
//
LOCUS       DATKGS010000020         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_15314, whole genome shotgun sequence.
ACCESSION   DATKGS010000020 DATKGS010000000
VERSION     DATKGS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15314"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VMQ48_00480"
     CDS             <1..1171
                     /locus_tag="VMQ48_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUD08552.1"
                     /translation="GERDVKSEEIIEDSSAATEEPTGPAQTMADFLNQTKLSFPKEGD
                     VIEGKVLEIGSNAVYLDLGVFGTGIVLGKEIKDGLGTTDKLKIGDMVSATVTDIEDEN
                     GFIELSVREASIEKAWLDLEEKQAGEAIFNVKILDANKGGLIVEVNGITGFLPVSQLS
                     NEHYPRVEEGEKSKILEKLRSYIGTEMSAKIIDTDRENEKLIVSEKAAYSAEEKKAIS
                     ELKTGDEIEGEVSGVVDFGAFVKFFPPSKADSIREEDRLEGLVHISELAWQLIDNPRD
                     IIKTGDRVKAKIIGIDNTRVSLSIKALLKDPWAAAEEKYVAGKTYDGQVRKINHFGAF
                     VYLDDDIHGLAHVSELLEAHPGKNIEEIIQTGNTYKWKILSIEPKEHRMGLALVK"
     gene            1202..1798
                     /locus_tag="VMQ48_00485"
     CDS             1202..1798
                     /locus_tag="VMQ48_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08553.1"
                     /translation="MQNSKLLKRVLVIGGPTGSGETTITNEIIKRFPVFTRLVTATSR
                     KPRNGEQNKIDYYFFSKEGFEKSIENGNILEYTYIENRNTYYGAYKPDLDEKIAKGFN
                     IVANVDIVGAKYYKETYNATAIFIKPQSIEELRGRLVNRDKDISPEELEKRLRNAENE
                     IEKEMPFYDYVVVNANGKIEEAVERTIEILKKENYSFV"
     gene            1813..>2137
                     /locus_tag="VMQ48_00490"
     CDS             1813..>2137
                     /locus_tag="VMQ48_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD08554.1"
                     /translation="MKIIKKIQENIFRYELFPRGAKVVVGVSGGPDSVCLLDALYKLQ
                     KKYNIELIIAHVNYGLRGKDSDLDEKLARELAEKYSLPIEVLKVGKVGKSEEKLRKTR
                     YAFFEK"
BASE COUNT          725 a          421 c          505 g          486 t
ORIGIN      
        1 gggagagagg gacgttaaat cagaagaaat aatcgaagat tcttcggctg cgactgaaga
       61 accgactgga cccgctcaaa cgatggcgga ttttttgaac caaacaaaat tatctttccc
      121 gaaagaaggc gatgtgattg aaggcaaagt gctggaaatc ggatcaaacg ccgtctacct
      181 cgacctggga gtttttggaa cgggaatcgt ccttggaaaa gaaatcaagg acggactcgg
      241 aacgaccgac aagctgaaaa tcggcgacat ggtttcggcc actgtcacgg acattgaaga
      301 cgaaaacgga ttcattgaac tttctgtgcg cgaagcttcc attgaaaaag cctggcttga
      361 tctcgaagaa aaacaagccg gcgaagcgat cttcaacgtg aaaattctcg acgcgaacaa
      421 aggcggactg atcgttgaag tgaacggcat aaccggattt ttgcctgtgt ctcaactttc
      481 aaacgaacac tatccgcgcg ttgaggaagg cgagaaaagc aagattttgg aaaaattgcg
      541 ttcatatatc ggaactgaaa tgtcggccaa gatcatcgac accgaccgtg aaaacgaaaa
      601 attgattgtg agcgagaaag cggcctatag cgccgaagag aagaaggcca tctccgaact
      661 caaaacagga gatgaaattg aaggggaagt gagcggcgtc gttgattttg gagccttcgt
      721 caaatttttc ccgccgagta aagccgactc gatccgcgaa gaggaccggc ttgaagggct
      781 ggtgcatatt tcggaactgg cttggcagct catcgacaat ccgcgtgaca tcatcaaaac
      841 cggcgaccgt gtgaaggcca aaatcatcgg catcgacaac acccgcgttt cgctttccat
      901 caaagccctt ttgaaagatc cttgggcggc tgccgaggaa aaatatgtgg ccggaaaaac
      961 ctatgacgga caagtccgca agatcaacca ctttggagct tttgtctatc ttgacgacga
     1021 catccacggc cttgcccacg tcagcgaact tctcgaagct caccccggaa aaaatatcga
     1081 agaaataatc cagaccggca atacatataa atggaaaatt ctttccattg aacccaaaga
     1141 gcaccggatg ggcttggcac tagtgaagtg aaaagtttaa agtgaaaagt gaaaaattac
     1201 aatgcaaaat tcaaaattat tgaaaagagt tcttgttata ggcggaccaa ctggaagcgg
     1261 ggaaacaacg atcactaatg agatcatcaa gcgattcccc gttttcacgc ggctcgtaac
     1321 tgctacttca cgaaaaccga gaaatggaga acagaataaa attgattatt attttttctc
     1381 gaaagagggg tttgaaaaaa gtatcgaaaa cggaaatatc ctcgaatata cttacataga
     1441 gaatcgaaat acttactacg gagcgtacaa gcccgatctc gatgaaaaaa ttgcaaaagg
     1501 ctttaacatt gtcgcaaatg tagatattgt tggggcaaaa tattacaaag agacctataa
     1561 cgcgacagct atatttatca agccgcaatc tattgaggag ttaagggggc gtttggtcaa
     1621 tcgcgataaa gatatcagcc cggaagaact ggaaaaaaga ctgcgtaatg ctgaaaatga
     1681 aattgaaaaa gaaatgccct tttatgatta tgtcgtcgtg aacgcaaacg gaaaaattga
     1741 ggaagcagtt gaaagaacca ttgaaatact aaaaaaagaa aattattctt ttgtctaatt
     1801 tatacaaact gtttgaaaat catcaaaaaa atccaggaaa atatttttcg ctatgaatta
     1861 tttccacgcg gagcaaaagt tgtggtcggc gtttccggcg gccctgattc agtttgcctt
     1921 ttggatgcgc tatataaatt gcagaaaaaa tataatatcg agttgatcat cgcgcacgta
     1981 aactatggcc tgcgcgggaa agattcggac ctggatgaaa agttagctag agaattagct
     2041 gaaaaatatt ctctgccaat cgaagttttg aaagtcggaa aagttggaaa gtcggaggaa
     2101 aaactaagaa aaactcgata cgcatttttt gaaaaag
//
LOCUS       DATKGS010000021         7600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_2585, whole genome shotgun sequence.
ACCESSION   DATKGS010000021 DATKGS010000000
VERSION     DATKGS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..388)
                     /locus_tag="VMQ48_00495"
     CDS             complement(2..388)
                     /locus_tag="VMQ48_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUD08555.1"
                     /translation="MEKKKILIVEDDNFVAEVYFAKLTEMGYEAILAQNGEEGIATLK
                     KDKIDMILLDILMPIMNGMEMLEEIKKREDWKNIPVILLTNVGEKESIQKVREMGVKN
                     YLIKSHFTPAEVIEKIESVFKENKTL"
     gene            complement(403..2004)
                     /locus_tag="VMQ48_00500"
     CDS             complement(403..2004)
                     /locus_tag="VMQ48_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HUD08556.1"
                     /translation="MVNPQNKEDKSEEKIDWEHLKIPAEVISLVPYEAAKKYQFIPFE
                     KEGRVLRVAGTDIDSVDVQNALQFLAEKNQLSIEMIPISEKDFDSALAGYTSPAFTIQ
                     QALETIGEDKEPAEKEKKEKKEDDVTIQEAPVAKIVEVILRNAIEGSASDIHIEPLED
                     SVRVRYRLDGILHNSLVLPKQIGPSIVSRIKILSNLKIDEKRKPQDGRFRITESKKQI
                     DLRVSTLPVSLGEKVVMRVLDKEKGLLDLEDLGVTGRNYDIIKKCIFEPYGMILITGP
                     TGSGKSTTLYSVLKILNREGVNIITLEDPVEYAIEGINQSQVRPEIGYTFASGLRSIL
                     RQDPDIIMVGEIRDKETAELAVHAALTGHVVLATLHTNDSLGAIPRLIDMEVEPFLVA
                     SSLRAVVAQRLVRKICPECREEVKMNDAVRQEIERVLEQIPEAEKKIHNLGKEIKIYK
                     GKGCAKCAESGMKGRIGIYEVFYMDEEVNDLLGGKFDEEKLRQIAQKQGMITMKQDGI
                     LKVLEGITTLEEVERVTEEGFLEIE"
     gene            complement(2029..2871)
                     /gene="pilO"
                     /locus_tag="VMQ48_00505"
     CDS             complement(2029..2871)
                     /gene="pilO"
                     /locus_tag="VMQ48_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HUD08557.1"
                     /translation="MMIQESMRLKVLILPLSLAIAVIVAIFFIKPAFSDMLSSKNALA
                     GKQAQLENLKNQNQKLQAMKTKWEAMADEKNLVATAFPETENVDSYISEITSKASRSG
                     VLLTDIKLGQAAGVGTSQNPDYICSSDSTGASTAASAQPVGAAAMPDTSGLQTLPGSS
                     SACLKAITISLTAKGSWEQMLDFFKYLEDMNRISNIEALTLSTQAQAQAQDQASSDIL
                     SADISANAFFKEKAQSGSMALSSNLASQGSFNQKSLDKLKETIYAPYDAPAVSPSGQR
                     NIFK"
     gene            complement(2889..3452)
                     /locus_tag="VMQ48_00510"
     CDS             complement(2889..3452)
                     /locus_tag="VMQ48_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08558.1"
                     /translation="MVTNIDLSSPEVAEKRSITGKSALIFSVLLLVFVLAAYLVVFFL
                     KSKYSASDKAISDEISQEQNKANGATFSDIIDFQGRLDSAGKIIDDHAYWDSMAKRMS
                     GYVIPEVKLTKFAGKKDSTGAGTIDISGTASSLDALSRELILLKDFPNLDSLEFKNAG
                     ESQGQTGQPSGIQFDASLKLDKSAFQK"
     gene            complement(3489..4571)
                     /gene="pilM"
                     /locus_tag="VMQ48_00515"
     CDS             complement(3489..4571)
                     /gene="pilM"
                     /locus_tag="VMQ48_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HUD08559.1"
                     /translation="MFGIFSKKNRCLGVDLGTTGIKIVELKKENNLPVFVNYAISYDS
                     GALLQSSGLELLDGQAKDILSKVLKEGGFTTKNAAIGIPGFFSLIFFVELPEMPDAEI
                     EQAVKFEAAKYIPTPIEEVSLGWEAIGSVQEKSFEGGQPSSQSRKMQVMVVSVPKSTT
                     VKYGGIVKSVKLSLKAMEVENFAVARAIIGNDKGTFMIVNIGAKATDFTVVSDGVVRI
                     TRNIDVGGAEISRSLASGFGIDLERADKLKKSGRINLLDPQNDTTRLIGPAIGIIIDE
                     MKRLREMYHKKSPLKKIEKMIFTGGTSKMATLTDYFAREIGMECQIGNPLARIGVDKR
                     QQAVVEAVSPELAVAIGLAMRGIEIE"
     gene            4837..5472
                     /locus_tag="VMQ48_00520"
     CDS             4837..5472
                     /locus_tag="VMQ48_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HUD08560.1"
                     /translation="MKILDFQVCPACEDSITDKGFLCFACRETRGLHLDGLIAAVSYE
                     DPAARKMVHNFKYRFVSDISEPLAKLICRALIRNDAMLPDFIVPVPLHPKRLRWRGFN
                     QSLLLAKHISEGLAPLMEIEILDILQRKKFNRPQMQIKNYQQRLKNMQNIFALLPDTN
                     LDLIKNKNILLIDDIATTGATLEECAKVLKSAGAKKVFAAVVARQSLKARN"
     gene            5388..6521
                     /locus_tag="VMQ48_00525"
     CDS             5388..6521
                     /locus_tag="VMQ48_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HUD08561.1"
                     /translation="MRQSSQIRRRQKSFRSGCGATKLESKKLVDYSEKTIYYGSMAER
                     HKSKRVKFPKNKQRDFLFTAKNKLGITWSEFAEILKISQRNLSDWKQEKITLPLSTVK
                     TICKKSGLAFPKNIQLLSPYWYTTKGASKGGLAVLKKYKIIGGDPQVRMKRWEEWWEK
                     EGKFKKHPILDRFPIKKPPFSAKLAEFVGILLGDGGISQRQVSITLNRETDKGYIQFV
                     KKMIKDLFGVSPGLYSHLKSKADAIVISRTELVEYLTQKLGLKIGNKVKQKIDIPKWI
                     MRKKEYQIACLRGLVDTDGTTIIHRYKSKGRSYVYKKLGFTSRSFPLLKSVNCILTEL
                     KIKNRIAKDQYNIRVEAVPDVKKYFDLVGSSNPKHLKRYLSGL"
     gene            6534..6624
                     /locus_tag="VMQ48_00530"
     tRNA            6534..6624
                     /locus_tag="VMQ48_00530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6569..6571,aa:Ser,seq:gct)
     gene            6661..6751
                     /locus_tag="VMQ48_00535"
     tRNA            6661..6751
                     /locus_tag="VMQ48_00535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6696..6698,aa:Ser,seq:tga)
     gene            6761..7300
                     /locus_tag="VMQ48_00540"
     CDS             6761..7300
                     /locus_tag="VMQ48_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08562.1"
                     /translation="MDHMKPKHEMARRAEHNVPANFSHSEEPVFSWEAHEYEYIEKHP
                     KWYWVMGLVLLAIVVYAIITNSILMAILFILIGMLGYVYAEREPRLIHMEINPDGVVA
                     DKIMYEYDDLKSFWIFYEVEAGFKVLSLHSKKTFLPHVHIPVGDANPILIREVLLNYL
                     PELRQEMTFVDRLSLMIGL"
     gene            7310..7383
                     /locus_tag="VMQ48_00545"
     tRNA            7310..7383
                     /locus_tag="VMQ48_00545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7344..7346,aa:Arg,seq:gcg)
     gene            7391..7462
                     /locus_tag="VMQ48_00550"
     tRNA            7391..7462
                     /locus_tag="VMQ48_00550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7424..7426,aa:Arg,seq:ccg)
BASE COUNT         2011 a         1852 c         1544 g         2193 t
ORIGIN      
        1 ttcataatgt tttgttttcc ttaaataccg actcgatttt ttcaatcact tccgccggcg
       61 tgaaatgcga tttgatgaga taatttttca ctcccatttc ccttactttc tggatgcttt
      121 ctttttcccc gacattggtg agaagaatca ccggaatatt tttccagtcc tcccgttttt
      181 tgatttcttc aagcatttcc attccgttca tgatgggcat caaaatgtcg agcaaaatca
      241 tgtcgatttt atcttttttg agagtcgcaa tgccctcctc cccgttttga gcgagaatcg
      301 cctcatatcc catttccgtc aacttggcaa aatacacttc cgccacgaaa ttgtcatcct
      361 ccacaattag aatttttttc ttttccatat ttttaaaata aattattcaa tttccaaaaa
      421 cccctcttcc gtcactcttt ccacttcctc aagggtcgtg attccttcca aaaccttgag
      481 tattccgtct tgtttcatcg tgatcattcc ctgtttttga gcaatttggc gcaatttttc
      541 ttcatcaaac tttcctccca aaagatcatt cacttcttcg tccatataga aaacttcata
      601 gattccaatt cttcctttca ttccggattc ggcgcatttg gcacatccct ttcctttata
      661 aatttttatt tcttttccaa ggttgtggat ttttttctcc gcttcgggaa tctgctccaa
      721 gactctttct atctcctgcc tcaccgcatc attcattttg acttcttcgc ggcattcggg
      781 gcagattttt ctgacaagcc tttgcgccac gaccgcccga agagaggaag cgaccaaaaa
      841 cggctccacc tccatgtcaa tgaggcgcgg aatggctccc aaggaatcat tcgtgtgcag
      901 ggtggccaga accacgtgtc ccgtgagcgc cgcgtggacc gcaagttcgg cggtttcttt
      961 gtcccgaatt tcgccaacca tgatgatatc cggatcctgc cgcaagatgg aacgcaatcc
     1021 cgaagcaaat gtatatccga tttccggcct cacttgactt tggtttatgc cttcaatggc
     1081 gtattcaacc ggatcttcaa gggtgattat gttgacgccc tccctgttga gaatcttgag
     1141 gaccgaatag agcgtggtgg atttccccga tcctgtcggc ccggtaatga gaatcatgcc
     1201 atacggctca aatatgcact tttttattat atcatagttc cgcccggtta caccgagatc
     1261 ctcaagatcc aaaagtccct tttctttgtc gagaacccgc atcaccacct tctctcccaa
     1321 cgagaccggc aaagtggaca cgcgaagatc gatttgtttt ttgctttccg tgatccggaa
     1381 ccggccgtcc tgcggctttc tcttttcgtc tattttcaga ttggaaagaa tttttattct
     1441 tgaaacgatc gagggtccga tttgtttggg aagaaccagg gaattgtgaa gaattccatc
     1501 caaccgatag cgcactctga cactatcttc caggggttcg atatgaatgt cgctggccga
     1561 tccttcgatc gcatttcgca agataacctc gacaattttc gccaccggcg cttcctgaat
     1621 cgtcacgtca tcttcttttt tttctttttt ttctttttca gccggctctt tgtcttctcc
     1681 tattgtttcg agggcttgct ggatcgtgaa tgccggactg gtataacccg ccaaagccga
     1741 atcgaaatcc ttctccgaga tagggatcat ttcgatgctc agctgatttt tttccgccaa
     1801 aaattgcaaa gcattttgca cgtccacact gtctatgtcc gttcccgcca cccggagaac
     1861 tcttccttct ttttcaaaag gaatgaactg atattttttc gcggcctcat aaggaaccag
     1921 gctgatgact tccgccggta ttttgagatg ctcccaatct attttttctt cacttttatc
     1981 ttctttattt tgaggattga ccatttttcc aaaccttgcg gataaaaatt atttgaagat
     2041 atttcgctgt ccgctcggag aaacggctgg cgcgtcatat ggcgcataga ttgtttcctt
     2101 caatttatcc agagattttt gattgaaact tccctggctg gccaggttgc ttgagagcgc
     2161 catgctgccg ctttgagctt tttccttgaa aaaggcattc gctgaaatat cagccgacaa
     2221 aatatcggag gacgcttggt cttgagcttg agcttgagct tgcgttgaca acgtaagcgc
     2281 ttcaatgttt gaaatccggt tcatatcctc aagatatttg aaaaaatcca acatttgttc
     2341 ccaactgcct tttgccgtaa gtgaaatcgt aatcgctttc aaacaggcac tcgaggaacc
     2401 gggcaaagtt tgcaatccgg aagtgtcggg catcgcagca gcgccgacgg gctgcgcgga
     2461 agcagccgtt gaagcgccgg tcgaatcact actgcaaata tagtccggat tttggcttgt
     2521 ccctacgccc gccgcctgac ccaattttat gtcggtcagc aaaactccgc ttcggctggc
     2581 ttttgacgtg atctcggaaa tataggaatc gacattttcc gtctccggaa atgcagttgc
     2641 gacaagattt ttttcatccg ccatagcttc ccattttgtt ttcatggcct gaagtttttg
     2701 gttttgattc ttcagatttt caagctgcgc ttgctttccc gccaacgcat tttttgagct
     2761 cagcatatcg gagaaagccg gcttgatgaa aaaaattgca actatgaccg cgattgccaa
     2821 tgaaagaggc aaaataagca ctttcagcct cattgattct tgaatcatca tattattaat
     2881 tttagtattt atttttgaaa agccgatttg tccaatttca agctggcatc aaattgaatc
     2941 ccgcttggct ggccggtctg cccttggctt tcccccgcgt tcttgaattc caatgaatcc
     3001 agatttggaa aatccttgag caaaataagc tcccgtgaaa gagcatccaa gctcgaggct
     3061 gttccgctga tatcaattgt ccccgccccg gttgaatctt tttttccggc aaatttcgta
     3121 agcttcactt ccggaatgac atagccgctc attcttttgg ccatactgtc ccagtaggca
     3181 tgatcgtcga taatctttcc cgctgaatcc aatctcccct gaaaatcaat aatgtcggaa
     3241 aaagtcgcgc cgttggcctt gttctgctct tgggatatct cgtctgaaat tgccttgtcc
     3301 gaagctgaat atttggattt caggaaaaaa accaccagat atgcagccaa aacgaaaacc
     3361 agcagcaaaa ccgaaaagat cagcgccgat tttccggtga tagatctttt ttcagctact
     3421 tccggagacg agagatcgat attcgttacc atttgttttt attttagatg ttagaaaaca
     3481 atatactttt attcaatttc aatcccccgc atagccagac cgatcgccac tgcgagttcg
     3541 ggagataccg cttccacaac cgcctgctgc cttttgtcca cccctatgcg agccaacgga
     3601 tttccaattt ggcattccat tcctatctct ctcgcgaaat aatccgtcaa ggtggccatt
     3661 tttgaagtac cgccggtgaa aatcattttt tcgatttttt tcaggggact tttcttgtga
     3721 tacatttcgc gaagcctttt catttcgtca atgattatgc ctatggccgg tccgatcaaa
     3781 cgggtcgtat cgttctgcgg atcgagcaaa tttatgcggc ctgatttttt gagcttatcc
     3841 gcccgctcaa gatcgattcc gaacccgctg gcaagagatc tggatatttc cgccccaccc
     3901 acatcgatat ttctcgtgat tctcactacg ccatcgctca ccaccgtaaa gtccgtggcc
     3961 ttggcgccga tgttcacgat catgaaagtt cctttgtcat tgcctataat ggcccgggca
     4021 acggcaaagt tctccacttc catcgctttc aggctcaatt tgacggattt cacaattcct
     4081 ccatatttaa ccgtcgtgct tttgggaaca gagaccacca tcacttgcat cttgcggctc
     4141 tgcgaggaag gctgcccgcc ctcaaaggat ttttcctgga cactgccgat tgcttcccac
     4201 cccagactga cttcttcaat gggggtcgga atatatttgg ccgcttcaaa tttcaccgct
     4261 tgctcgattt ccgcatcggg catttccgga agctcgacaa agaaaatcaa agaaaagaat
     4321 ccgggaattc ctatggcggc attttttgtg gtgaaacctc cctccttgag cactttggaa
     4381 agaatatctt tcgcctgccc atccaaaagc tccagcccgc tggactgcaa aagtgctccg
     4441 gaatcatagg aaatcgcata gttgacgaaa acaggcaaat tgttctcctt tttcaactca
     4501 acaattttta tgccggtcgt cccaagatcc acccccaaac atctgttttt ttttgaaaat
     4561 attccaaaca tcaaaatttc ggatgcatag tttttatttt tgtaccccgc ctgcttgtgt
     4621 tattttaaca catttttctt ttgaaatcaa actgaaaaac atttttgtgc taaaatcagt
     4681 atagcataaa aagaaaacaa aaagaatatc ttgtccacag cccctttttc gaaaataaga
     4741 tccctgaaaa acgtcgctct tgattcgctt ttccctattt tttgcctttc ttgccgtaaa
     4801 gaaggttttt ggctgtgcga aaattgcctg gcggagatga aaattttgga ttttcaggtt
     4861 tgccccgctt gcgaagacag catcaccgac aaaggttttt tgtgctttgc ttgccgggaa
     4921 acacgcggat tacaccttga cggcttgatt gccgccgttt cctacgagga tcccgccgcg
     4981 cgaaaaatgg tacacaactt caaatatcgt ttcgtcagcg atatatcgga gccccttgcc
     5041 aagctgatct gccgcgcgct catccgaaat gatgcgatgc ttcctgattt cattgttcct
     5101 gttccgcttc atcccaaaag actgcgctgg cgaggattca atcaaagcct cttgctcgcc
     5161 aaacatattt ccgaaggact cgcgccgctg atggagatag agattttgga tattttgcag
     5221 cggaaaaaat tcaatcgccc gcaaatgcaa atcaaaaatt accagcagcg gttgaaaaat
     5281 atgcaaaata ttttcgcctt gttaccggac acaaatctcg atttgataaa aaacaaaaat
     5341 attcttttga tcgacgacat tgcgactacc ggcgccacgc ttgaagaatg cgccaaagtt
     5401 ctcaaatccg ccggcgccaa aaaagttttc gcagcggttg tggcgcgaca aagcttgaaa
     5461 gcaagaaatt agttgactat tcagaaaaaa caatatatta tggaagcatg gctgaaaggc
     5521 ataaatcaaa aagagttaaa tttccaaaaa ataagcaaag ggattttctc tttactgcga
     5581 aaaacaagct cggtataact tggagtgaat tcgccgaaat actgaaaata agtcagagaa
     5641 acctttcgga ttggaaacaa gaaaaaatca ctcttccctt gagtaccgtt aaaactattt
     5701 gcaaaaaatc aggtttggct tttcccaaaa atattcagct gctttcaccc tattggtata
     5761 cgacgaaagg cgccagcaaa ggaggactcg ctgttttgaa aaaatacaaa attatcggcg
     5821 gtgatcctca agttaggatg aaaagatggg aggaatggtg ggaaaaagaa ggaaaattca
     5881 aaaaacatcc aattcttgat agattcccca ttaaaaaacc gcctttctcc gctaagcttg
     5941 cagaatttgt cggtatattg ctgggagacg gcggaataag ccaaagacaa gtgtcgatca
     6001 cattaaaccg cgaaactgac aaagggtata tccaatttgt gaaaaaaatg attaaggatt
     6061 tgttcggagt ttcccctgga ttgtattcac atctcaaaag caaagcagat gcaattgtca
     6121 tttctcgcac agagctggta gaatatctaa cgcaaaagct agggctaaaa ataggcaata
     6181 aagttaaaca aaaaatagat ataccgaaat ggattatgcg caaaaaagaa taccagatag
     6241 cctgtttgcg cggcttagta gatacggacg gtactacaat aattcaccgg tataaatcaa
     6301 agggtcgatc ctatgtttac aaaaagttgg gctttaccag cagatcattt cccctcctca
     6361 aatccgtcaa ctgtatacta acggaactaa aaataaaaaa taggattgca aaagaccaat
     6421 acaacataag agttgaggca gtgcctgatg taaaaaaata ttttgacctg gtcggctcca
     6481 gcaatccgaa acacttgaaa agatatctat ctgggctata atcaaaaaac cggggagagg
     6541 tgcctgagtt ggcttaaagg agcatcctgc taagatgttg actccgcaag gggtcccagg
     6601 gttcgaatcc ctgcctctcc gccaagaaaa atacacttat caagagccgt tattaataaa
     6661 ggtgaggtac cgaagtggtc ataacggggc agtcttgaaa actgcttgtt ccgcaagggg
     6721 cacgtgggtt cgaatcccac cctcaccgcc aagaaaactt atggatcaca tgaaaccaaa
     6781 acacgaaatg gcaagacgcg ctgaacacaa tgttcccgcg aatttttccc actccgaaga
     6841 gccggttttt tcttgggaag cccatgaata cgaatacatt gaaaagcacc cgaaatggta
     6901 ttgggtaatg gggctcgtgt tgcttgccat cgtcgtctat gctatcatca ccaactccat
     6961 tcttatggcc atcctgttca tcttgatcgg aatgctcgga tacgtgtacg ccgagcgcga
     7021 accgcgcctc atccatatgg agatcaaccc cgacggcgta gtcgcggaca aaatcatgta
     7081 cgagtacgat gatctcaaga gcttctggat attctatgaa gtggaagccg gtttcaaagt
     7141 tttgagtctc cacagcaaaa aaaccttcct gccgcacgtt cacattcccg tcggcgacgc
     7201 caatccgatc ctgatccgcg aagtacttct gaattatttg cccgaacttc gtcaggaaat
     7261 gacttttgtc gacaggcttt ctttaatgat agggttgtaa aaatatgacg cgctcgtagc
     7321 tcagctggat agagcgttgg cttgcggagc cagaggccac aggttcgaat cctgtcgagc
     7381 gcacaaattc gtctccatag ctcaatggat agagcgcagg cctccgaagc ctgaaataaa
     7441 ggttcgactc cttttgggga cactatggat caccttcaaa cttcactctc acaaatctct
     7501 ttcatagaaa aaaattactt gaaaaagttg gaaaagctcg ggcttgagac cgtccgtgat
     7561 tttttgtatt ttttcccgaa ccgctacgac gatttttcaa
//
LOCUS       DATKGS010000022         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_30043, whole genome shotgun sequence.
ACCESSION   DATKGS010000022 DATKGS010000000
VERSION     DATKGS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30043"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..316
                     /locus_tag="VMQ48_00555"
     CDS             17..316
                     /locus_tag="VMQ48_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08563.1"
                     /translation="METETKEEILERRKEIEREILEMLEETESDFGLEDVKEAIYNEE
                     EQDDFTHIVAMFDRGGDASELSNVLELITDAWNYFPHKALGGLSPAEESMERRKK"
     gene            589..1410
                     /locus_tag="VMQ48_00560"
     CDS             589..1410
                     /locus_tag="VMQ48_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUD08564.1"
                     /translation="MKLNPKTELTVKTALFSAVFTLVLFSLANFFVNRSWNFFSTNNS
                     KTQPFTVQGSGSATATPDQAQISFTVTKTAPKLEDAQNEANAATNSIVTDLQSAGIQK
                     KDIQTGNYNSHPNYTESTSTISSMPIRPMPIPLPGNSTDIASYTVDENITITLHDISK
                     ASAAIDIATKGSAENIYGPSFTFSEQEQQTLSDQARTVAISNAKQKAEKMAGETGIRL
                     GKILSVQETNTPFGIQPLMMNAKTESGASSGSAPTQINPGENTMTVNIVLSYEVR"
     gene            1521..1889
                     /locus_tag="VMQ48_00565"
     CDS             1521..1889
                     /locus_tag="VMQ48_00565"
                     /inference="COORDINATES: protein motif:HMM:NF039180.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HUD08565.1"
                     /translation="MTSFATFLATHQWILILAAIWTIPWKGVALWRAARNRSVSWFVV
                     LLIVNTLGILEIIYIFAFSKKGKEEEPQPIETPEIPTPEKIVLDIKGEEKAEEKTEAK
                     EEEEKKEEEKSSLDEEIPKS"
     gene            1939..2211
                     /locus_tag="VMQ48_00570"
     CDS             1939..2211
                     /locus_tag="VMQ48_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HUD08566.1"
                     /translation="MSKMNIQPLGENVLIEPAKSDTKTASGIVLPDSNGEKPQEGLVI
                     AIGDDKKIKVKAGQKVIYSKYSGNEIKMGGNEYLLVKNEDILAIVK"
     gene            complement(2264..3094)
                     /locus_tag="VMQ48_00575"
     CDS             complement(2264..3094)
                     /locus_tag="VMQ48_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUD08567.1"
                     /translation="MQNEKNQHKVFVFGFLLIVLVVLGFLLKPVVMNWKDGQKNQAEQ
                     KANAEILKAPSVMPDDLFQMIQDKSKVFLVDISAPDDFKRGHIALAVNVPMEKLDKSF
                     ISSLGAEKTANIFIVNQGSDLAALATVTNKIVSDGFVNAKYLRGGISGWIEKGFPLVS
                     LGGSEEDNAKVKKITIDEIKNDAGSTPDLLQFLDVRSKDDFAKEHIVGALNIPLTELE
                     TRKDDIPAVKKIIVYGSDENESFQAAVTLFDLNFFNVWQMDGGINEWKAAGGNIASGK
                     "
BASE COUNT          984 a          759 c          626 g          791 t
ORIGIN      
        1 atagccaagt taaaaaatgg aaactgaaac aaaagaagaa attttggaaa gacggaaaga
       61 aattgagcgg gagattttgg aaatgctcga ggaaactgaa agcgattttg gattggagga
      121 tgtcaaagaa gcgatttaca acgaggaaga gcaggatgat tttacgcata tcgtggctat
      181 gttcgatcgc ggaggtgacg cttctgaatt gagcaatgtt ttggagctta ttaccgacgc
      241 atggaattat tttccgcata aagctcttgg cggcctttcg ccggccgaag aaagcatgga
      301 gcgtagaaaa aaataatatt ttttgattcg tattattgca tattgaacac gaggccgatt
      361 tgttttcgag tataccaatc aagaaaatca acgcttctcc atcaacacgc tcaccctttc
      421 catctgcacc tcgacccact cccaatgctc caatattccc tcgtatgata tactgaaatc
      481 gttgcccgat gtatcctcat atgccagccg aagaatcgat tttagcaatt caataatcgt
      541 ggtataatca aggcaatcag aggtttctaa aaaaataaac aaaaaaatat gaaattgaat
      601 ccaaaaacag aattgacggt caaaacggcg cttttttcag cggttttcac gctcgttttg
      661 ttttcgctcg ccaacttttt cgtgaatcgc tcctggaatt tcttttccac gaacaattca
      721 aaaactcaac cattcaccgt tcagggctcc ggcagcgcca cggcaacccc cgaccaggcg
      781 caaatatctt tcactgtcac aaaaacagcg ccaaagctcg aagacgcgca aaatgaagca
      841 aatgcagcca ccaattccat cgtgaccgac cttcaaagcg ccggaatcca gaaaaaagac
      901 attcagaccg gcaattataa ttctcatccc aattacaccg agagtacttc gacaatttcg
      961 agcatgccga tccggccaat gcccattcct ctgcccggaa attcaactga catcgcaagc
     1021 tatacggtcg atgaaaatat aaccatcact ttgcatgata tttccaaggc cagcgcagcg
     1081 atcgacattg ccaccaaagg atccgcggaa aacatctatg gtccgagctt cactttctct
     1141 gaacaggagc agcaaacgct ctccgatcag gccagaacgg ttgccatatc gaacgccaaa
     1201 cagaaagccg agaagatggc gggggagacc ggaattcgcc tcggaaaaat attgagtgtt
     1261 caggaaacca acactccctt tggcattcag ccccttatga tgaacgccaa aaccgaatca
     1321 ggtgcttcat ccggatcagc gccgacacag atcaacccgg gtgaaaatac catgacagtg
     1381 aatatagttc tgtcctacga ggtacgttga aaaatcaagt atgtgtcttt tttgctttac
     1441 aaatccatat attccggtat aatgactata ctcgaacttc atttcgcccc accagattaa
     1501 ctcattatat caacaaaaac atgacgagtt tcgccacatt tctcgcaacc caccaatgga
     1561 tccttatcct cgccgccatt tggaccattc cctggaaagg cgtggcgctt tggcgggcgg
     1621 ccaggaaccg gagcgtctct tggtttgtcg ttttgctcat cgtcaatacg cttggaattc
     1681 ttgagataat ttatattttt gctttttcta aaaaaggaaa ggaagaagag ccgcagccga
     1741 ttgaaacacc ggaaatacca acaccggaaa aaatagtttt ggatataaaa ggggaagaga
     1801 aagcagagga aaaaacagaa gcaaaagaag aggaagagaa aaaagaagaa gaaaaatcaa
     1861 gtcttgacga agagattcca aaaagctaag atatatatct gagagcaaga tcaataatca
     1921 ctaaaccaaa aaacgcatat gtctaaaatg aacatccagc ctctcggcga aaacgtcctc
     1981 attgaacccg ctaaatcaga cacgaaaacc gcttccggca tcgttctccc tgattcaaat
     2041 ggagaaaaac cgcaagaagg actggtcatc gcaatcggtg acgacaagaa gatcaaagtc
     2101 aaggccggac aaaaagtgat ctactccaaa tattctggga atgaaatcaa gatggggggc
     2161 aatgaatatt tgctggtgaa aaacgaggat atcctggcga tagtgaaata aattttttag
     2221 gaacgataaa aagcggaata aaatccgctt ttttatttga atattatttt ccacttgcga
     2281 tatttcctcc cgccgctttc cattcattga tcccgccgtc catctgccaa acattgaaaa
     2341 aattcaagtc aaacaaagtc accgccgcct ggaaactttc gttttcgtcc gatccataga
     2401 caataatttt tttcacagca ggaatatcgt cttttctggt ctcaagctcg gttaggggaa
     2461 tattcaacgc tccgacaata tgctctttcg caaaatcatc tttgctgcgc acatccagga
     2521 attggagaag gtcaggagtg ctcccggcat catttttgat ctcatcgatc gttatctttt
     2581 tcaccttcgc gttgtcttcc tcggaaccgc ccaaggaaac cagcggaaag cccttttcaa
     2641 tccagccgga aattccgccc cgcagatatt tggcgttgac gaatccatca gaaacgatct
     2701 tgttggtgac tgttgccaag gctgccaaat cgcttccttg attcacaatg aaaatattgg
     2761 cggtcttttc cgcgcccaga ctgctgataa aacttttgtc caatttttcc atcggcacat
     2821 tcaccgccag cgcaatatgc cctcgcttga aatcatctgg cgcgctgata tccaccaaaa
     2881 aaacttttga tttgtcctgg atcatttgaa acagatcatc tggcatcacc gaaggcgcct
     2941 tcaaaatttc cgcgttggct ttttgctcag cctgattttt ttgtccgtct ttccaattca
     3001 tcaccaccgg cttcaagaga aaaccaagga ccaccagcac gatcagcaaa aaaccgaaaa
     3061 caaatacttt gtgttgattt ttttcgtttt gcatattttt atctcaaaat ttccccgaca
     3121 aattcgggaa accccctcac aaaatcattg acatccatcc
//
LOCUS       DATKGS010000023         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_175107, whole genome shotgun sequence.
ACCESSION   DATKGS010000023 DATKGS010000000
VERSION     DATKGS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175107"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..414)
                     /locus_tag="VMQ48_00580"
     CDS             complement(91..414)
                     /locus_tag="VMQ48_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08568.1"
                     /translation="MGDYNVAFRYTRKAGAYHGVVNWTSFSSREEFQQMYTPERQEHQ
                     EVVEEGITEERAIQLTFQTPLSCRLAASIGEATDEAGNINPTLLEVLLANSIYAEREA
                     RKRGC"
     gene            complement(697..1812)
                     /locus_tag="VMQ48_00585"
     CDS             complement(697..1812)
                     /locus_tag="VMQ48_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HUD08569.1"
                     /translation="MFGSRNSERVGSSKKRFYKTKKFKTFVVVLALILVIIGYFGWKT
                     GAILNKITGGRSNALSSLLGGSSNPQGQEGRINVLLLGDTEGRAVAAGDLLADSIILV
                     SLDTKDNKVAMISIPRDLYVKIPETNDRGKLNSVYAYWQGGGKNQGIPKMEEMVGTIT
                     GLKVDYAIVISGNGFQQLVDAVGGIDIHLQKAFYETQQFVAGNECGGVFTLPAGTNHL
                     DGAKALCYARARDETSDFDRSKRQQVMLKALKDKMLSIGTLTDFNKLNNILNTVGNNV
                     KTDLTPDQMKGLYDQYNKMQDAQIVQRVFENSPQGLLEVPAAAPNLGYVLFPIAGEDN
                     YSQLQDACRNIFTEAAQSDTNPVVQSGAPAAPAPVKK"
     gene            complement(1931..>2938)
                     /locus_tag="VMQ48_00590"
     CDS             complement(1931..>2938)
                     /locus_tag="VMQ48_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08570.1"
                     /translation="VLLIALVFNLGQLIYEAKSNFENSKVFFHSIAQPDPKSTNSLPL
                     KLAMDADCQLQANFHIMSSLGDKVSCDYSLITLTDPKISLFSLNSIGDYYGCAVNFLG
                     TTGIYIRNLLLFLEIIFSIFGYGLLIYRFRKESGKKKQFLGLAILYCLVSFLVIMPVI
                     GDTPLRYFLLSFFAPYIFLGLLGEYAIKRFRFGIFLVASIFLFLVASNYFSLKSVAED
                     LAAGKRSGSSVCGPDAIVLGEIEPMAGYIAHESYPQNEAYILHTSRYTFLNPIKYLTQ
                     TGGINLSLANGRGFNNIPPGKPLFFFSENFFADPNFPIYGRKIESCQKFARVTIYKLQ
                     N"
BASE COUNT          812 a          664 c          643 g          819 t
ORIGIN      
        1 ggggagagga ggggttcgca ggcgacaaat gaaaaaggcg acctcaataa gacgatcgcc
       61 tttaaatgta gctggcagat gtcaaccttg ttaacagcca cgctttcgag cctctctttc
      121 cgcgtaaata gaattggcga gcaacacctc tagaagtgtt ggatttatgt ttcccgcttc
      181 atcggtcgct tccccaatac ttgcagccaa tctgcatgaa aggggcgttt gaaacgtgag
      241 ttggatagcc cgctcctccg taatgccctc ctcaacgact tcttgatgtt cctgcctttc
      301 gggagtatac atctgctgga attcctcccg gctggagaag cttgtccaat tcacgacgcc
      361 atgataagcg cccgctttcc gtgtataacg aaaagcaaca ttataatctc ccataacctc
      421 accccctcca aaatgattgt gaaaagttat ttcgataaat attgctactt tatttttaac
      481 acattcctaa atgttattaa aatgcgttat agtaatctaa cgcaatatgc aaaaagtgtc
      541 aagctctgta ttttttattg gcaaaatccc aactcttgtg tagaatagca atattgggca
      601 gctagcgttc tcacttcgtc ccaggctgcc cgcagtttga gcgaaaaaaa caagactcat
      661 attgagcctt gtctttttct tttgattata ctaagcttat tttttcacgg gagctggagc
      721 tgctggcgcg ccgctttgta cgaccggatt tgtgtctgat tgcgctgctt cggtgaagat
      781 attccggcag gcatcttgca attgggaata gttgtcctct cccgcgattg ggaacaagac
      841 gtagccgaga ttcggtgcgg cggcgggaac ttccaaaagt ccctggggag aattctcaaa
      901 taccctctgg acgatctggg cgtcttgcat tttgttgtac tggtcataaa gtcccttcat
      961 ttgatccgga gtaagatcag ttttcacatt gtttccaaca gtgttcagaa tgttgttgag
     1021 tttgttgaag tcggtcagcg tgccgatgga gagcatttta tctttcaagg ctttcagcat
     1081 aacttgctgt ctctttgagc ggtcaaaatc ggaggtttca tcacgggctc gggcgtagca
     1141 aagagctttt gctccatcca ggtgattggt tccggccgga agcgtgaaaa cgccgccgca
     1201 ttcatttcct gcgacaaact gctgagtttc atagaaggct ttttgcaaat ggatgtctat
     1261 tcctcccacc gcatccacca attgctggaa tccattgcca ctgatgacga ttgcatagtc
     1321 aactttgagt ccggttatgg tgccaaccat ttcctccatc tttgggatgc cctgattttt
     1381 tccgcctccc tgccaatatg cgtacacgga attaagcttt ccccggtcgt tggtttccgg
     1441 aatctttacg tatagatccc gagggataga aatcatggcc actttgttat ctttcgtgtc
     1501 gagagatacc agaatgattg agtcagccaa caagtcgccg gcagcgacag cccttccttc
     1561 cgtatcgcca agaagaagaa cattgatacg gccttcctgt ccctgcgggt tcgatgatcc
     1621 gcccagcaag ctggacaagg cattgctccg tccgcccgtt attttgttga gaatagctcc
     1681 tgttttccaa ccgaaatacc caatgattac caaaataaga gccaagacga ccacgaaagt
     1741 tttgaacttt ttcgttttgt agaatctttt tttggatgac ccaactcttt cgctatttcg
     1801 actgccaaac atagtttttg cttaatattt agactaatat gactaacaaa ttgtattcta
     1861 caaaatatac ggccaatggg caagaaaaaa ttcagattgt caagccgtgg cctatgggcg
     1921 aaaataaatt ctagttttga agcttataga tggtaactct ggcaaatttt tggcagcttt
     1981 caattttgcg tccataaatc gggaaattcg ggtcagcaaa aaaattttcc gaaaaaaaga
     2041 aaagcggttt tcccggagga atgttgttaa aacctcttcc gttcgccaag cttagattta
     2101 taccacctgt ttgcgtcaga tatttgatcg gattgagaaa ggtgtatcgg ctggtatgca
     2161 aaatatacgc ctcgttttga ggataggatt catgagcgat atagccggcc atcggttcta
     2221 tttcaccaag aacaatggca tccggtccgc acaccgaaga gccgcttctt ttccccgcgg
     2281 caagatcctc ggcgacggat ttcagagaga aatagttaga cgcgaccaag aacagaaaaa
     2341 ttgaggcgac taagaaaatg ccaaaccgga atcttttgat tgcatattct cccagaagac
     2401 ccaaaaaaat atacggcgca aagaagctca aaagaaaata tctaagcgga gtgtcgccga
     2461 taaccggcat tatcaccaga aaagaaacta agcaataaag aatagctaaa cccaaaaatt
     2521 gtttcttctt tcctgattcc tttcggaaac gataaatcag caatccatag ccgaaaatgg
     2581 aaaaaataat ttccaaaaaa agtaaaagat tccttatata tattccggtc gttcccaaaa
     2641 aattgacggc gcaaccataa tagtccccaa tgctgttgag ggaaaacagg gatattttcg
     2701 gatcagtcag cgtgatcagg gaataatcgc aactcacctt gtctcccagg gaagacatta
     2761 tgtgaaaatt ggcttgaagc tggcaatccg cgtccatagc cagctttagc ggcaagctgt
     2821 ttgtactttt cggatcgggt tgagcgattg agtgaaaaaa aacttttgaa ttctcgaaat
     2881 tgcttttggc ttcataaatc agctgtccga gattaaatac gagagcaatg agcaggac
//
LOCUS       DATKGS010000024         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_45709, whole genome shotgun sequence.
ACCESSION   DATKGS010000024 DATKGS010000000
VERSION     DATKGS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45709"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /gene="ftsH"
                     /locus_tag="VMQ48_00595"
     CDS             complement(<1..1601)
                     /gene="ftsH"
                     /locus_tag="VMQ48_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUD08571.1"
                     /translation="MKSFLKNLVIILLVFLVISGIVMLFNTPAPQAQNITLSELATQI
                     NDGKVKDILVNGDGLDITLVDNTQEKSRKEADAALTDSFKNYGVDQSALRAVNINIKD
                     TTSTAFWVNTVIPFLLPFIIIGAFLWYMMRSAQRGNNQALSFGMSRARLISPKKNGKK
                     KITFADVAGAEEAKDELKEIVEFLKHPKKFLDLGARIPKGVLLLGSPGTGKTLIAKAV
                     AGEANVPFFSISGSEFVEMFVGVGASRVRDLFKQAKKSAPAIVFIDEIDAVGRHRGAG
                     LGGGHDEREQTLNQILVEMDGFDTDTNVIVIAATNRPDVLDPALLRPGRFDRRVVMDL
                     PDIKEREAILKIHTKNMPIAKGVDLHKIAARTPGFSGADISNLVNEAAILAARRGKKV
                     VEDDELRVSIEKVILGPERKSKILNVQEKEITAYHEAGHALIASSLKNADPVQKVSVI
                     SRGQAAGYTLNVPQEDKRLYTRAYFIDELAVFLGGYVSEKIFFGDVTTGASNDLERAT
                     DTARKLVTRYGMSNLGPRTFGHKEEL"
     gene            complement(1610..2629)
                     /gene="tilS"
                     /locus_tag="VMQ48_00600"
     CDS             complement(1610..2629)
                     /gene="tilS"
                     /locus_tag="VMQ48_00600"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HUD08572.1"
                     /translation="MNLIKKIQENIFRYELFGCRASAKGGSASGGKIVVGVSGGPDSI
                     CLLDVLYKLKKKYDFKLIVAHVNYGLRGKDSDLDEKLVRKMAEKYSLPIEVLKVGKVG
                     KSEEKLRKTRYAFFEKVSKKHRAGTIAVGHNLNDQAETVLMRILRGTGLRGLGAIKFS
                     TRRGPTAQPKRNADRPLLVRPLLNVPRKEILAYLRKNKIAYRIDKTNLGTDFTRNKIR
                     NKLIPQLEKKFNQNIQESLFKFSESAAADYDFISRFSKVWLTANKVLMVSSLNSLHPS
                     ILNEVLRQTIEKYNPNLREIESAHIEEINKILKSNKNKRQQITLKGLKIGRIGDKLTI
                     EKINP"
     gene            complement(2626..>3161)
                     /locus_tag="VMQ48_00605"
     CDS             complement(2626..>3161)
                     /locus_tag="VMQ48_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08573.1"
                     /translation="GPSGAGEDSVIEGLKKYFQIERVITTTTRSMRAGESQGKPYYFI
                     SHEEFKKKIERDELAEYAQEYNDNFYGVAKDELDRVRNSGNIGLWKIEYKGVITAKKK
                     FPEIKSIYIAPPSLEVLRQRILKRDPDVSEEYLKERMDYTKEWMKYENVYTYKVVNEE
                     GKLDETIEKVADIIRKQ"
BASE COUNT          718 a          743 c          738 g          962 t
ORIGIN      
        1 agttcttctt tgtgtccgaa agtgcgaggt ccgagattgc tcatcccata tcttgtgacg
       61 agttttcgcg ccgtgtcggt tgcccgttcg aggtcattcg aggcgccggt cgtcacatcg
      121 ccgaagaaga ttttttcgct cacgtatccg cccaaaaaca cggccagttc gtcgatgaag
      181 taggcgcggg tatagagccg cttgtcttct tgcgggacat tcaaggtata cccggcggct
      241 tggccgcgcg aaatgacgga aactttttgc accggatcgg cgttcttgag acttgaagca
      301 atgagagcgt gcccggcttc gtgataggcg gtgatttctt tttcttgcac gttcaaaatt
      361 ttgctctttc gctctggacc aagaattact ttctcgatgg aaacgcggag ttcgtcgtct
      421 tcaacgactt tctttccgcg ccgggcggcc agaatcgccg cttcattcac gagattggaa
      481 atatccgctc cggaaaaacc gggagtgcgc gccgcgattt tgtgaagatc aactcctttg
      541 gcgatgggca tgttctttgt gtgaatcttg aggatagctt cgcgttcttt gatatccggc
      601 aggtccatca cgacccggcg gtcgaaccgg cccggccgca ggagcgccgg atcaagaaca
      661 tccggccggt tggtggcggc aatcacgatc acattcgtgt ctgtgtcaaa gccgtccatc
      721 tcaacaagaa tctggttgag agtttgttcg cgttcgtcgt gtccaccgcc aagaccggct
      781 ccccggtggc ggccgaccgc gtcaatttca tcaatgaaga caatcgcagg agcgcttttt
      841 ttggcttgtt tgaaaagatc gcgcacccgg ctggcgccga cgccgacaaa catctccaca
      901 aattccgaac cggagatact gaagaaggga acattggctt caccggcgac ggctttggca
      961 atgagcgttt ttcctgttcc gggagagccg agaagcaaaa cgcctttcgg gatacgcgcg
     1021 ccaagatcca aaaatttttt cgggtgtttc aaaaactcaa caatttcctt caattcgtct
     1081 ttggcttcct ccgctccggc gacatccgcg aaagttattt ttttcttgcc gttctttttt
     1141 ggagaaatga ggcgggctcg ggacattccg aaagagagtg cctgattgtt tccgcgttgg
     1201 gcggagcgca tcatatacca gaggaaggcg ccaataataa tgaacggcaa aagaaaagga
     1261 atgaccgtgt tgacccaaaa agcggtcgaa gttgtgtcct tgatgtttat attgactgcg
     1321 cgaagagcac tctggtcaac gccataattt ttgaacgaat ccgtgagcgc cgcgtccgct
     1381 tctttgcgtg atttttcttg ggtgttgtcg acgagagtga tgtccaaacc atccccgttc
     1441 acaaggatat ctttcacttt tccgtcgttg atctgggtgg ccagttcact caacgtgatg
     1501 ttttgcgctt ggggtgccgg agtgttgaaa agcatcacta tgcccgaaat aacaagaaaa
     1561 acaagaagga tgatgactaa atttttgaga aaacttttca taagatattt tatggattaa
     1621 ttttttctat agtcagctta tcaccgattc tccctatttt caagcccttg agggtgattt
     1681 gctgtctctt attcttatta cttttcagaa tcttattaat ttcttcgata tgtgcggatt
     1741 caatttcccg cagattcgga ttgtattttt cgatcgtctg gcggagaact tcgtttagga
     1801 tcgacggatg gaggctattc agtgatgata ccatcaagac cttatttgct gtgagccaaa
     1861 ctttcgaaaa acgcgagatg aaatcatagt cggccgcggc tgattcggaa aatttgaaga
     1921 gtgattcctg gatattttgg ttgaattttt tttctagctg tgggatgagc ttgttgcgaa
     1981 ttttgtttcg ggtgaaatcg gtgccgagat tggttttatc gatccggtac gcgattttat
     2041 tcttacgtaa gtaagccagg atttctttgc ggggtacgtt gagaagcggc cggacgagta
     2101 gaggtctgtc cgcattcctc tttggctgcg cggtcggacc tctacgggtt gagaatttga
     2161 tcgcgcccaa tcctcgcaac cccgtcccgc gcagaatccg catcaaaacc gtttcagctt
     2221 ggtcgttcag attgtgtccg acggcgatgg taccagctct atgttttttc gaaacttttt
     2281 caaaaaatgc gtatcgagtt tttcttagtt tttcctccga ctttccaact tttccgactt
     2341 tcaaaacttc gattggcaga gaatattttt cagccatttt gcggactaat ttttcatcca
     2401 aatctgaatc ttttccgcgc aggccataat tcacgtgcgc gacaatcaat ttgaaatcat
     2461 attttttctt caatttatat agcacatcca aaagacaaat cgaatccggc ccgccggaaa
     2521 cgccgaccac gatttttccg ccagaggcgg atccgccttt ggcggacgct ctacatccaa
     2581 aaagttcgta gcgaaaaata ttttcctgaa tttttttgat gaggttcatt gtttccttat
     2641 aatgtcagcc actttttcga ttgtctcatc taattttcct tcttcgttta caactttata
     2701 agtatagacg ttttcgtatt tcatccattc cttagtataa tccattcttt cttttaaata
     2761 ttcttcggac acgtcaggat ctctttttaa gattctttgc ctcaaaactt caagagatgg
     2821 cggtgcaata tagattgact ttatctccgg aaattttttc ttggcggtta tcactccttt
     2881 atactcaatt ttccacaagc caatattgcc ggaatttcgc acacgatcca attcgtcctt
     2941 tgcgacgccg taaaaattat cgttgtattc ttgcgcatac tcagcaagtt cgtctcgttc
     3001 gatctttttt ttgaattctt catggctgat aaaataatag ggcttgcctt gcgattcgcc
     3061 tgcgcgcatt gaacgagtgg tcgtggtgat taccctttcg atttggaaat attttttcag
     3121 accctcaatg acagagtcct ctccagcgcc cgatgggcca g
//
LOCUS       DATKGS010000025         7103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_58829, whole genome shotgun sequence.
ACCESSION   DATKGS010000025 DATKGS010000000
VERSION     DATKGS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58829"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2573)
                     /locus_tag="VMQ48_00610"
     CDS             complement(<1..2573)
                     /locus_tag="VMQ48_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08574.1"
                     /translation="MKMKKNKKIIVSMFSVFALTLNLVLPFAAQAYTVGLPSLSNGGS
                     VNIDVNQILQEAQYSSISQISQNAATGITNSTEQRYGISSDIFRSAERKADAPRAEVF
                     FDNTNPKVGEKVTAHAVPEFFKNDPQNLYYTWYIMHTTDGRIQTATNSIKEGKTEAAG
                     IMARGDYDPNLDGQTYADPGKDPDNDGWPAVDTNSYDSNTCAAPMGGSDGVGGLADQT
                     VDSYKTSTDWCDSLGGHAWDNCSYNDDTSSRPLDSYFTLKSSQHNHYCDLCKDYFSGD
                     GATAFASAKTTRNNCCYANTPQSSLQCSTTTQVTDPDTGAVSDVTTYSKCPQSSGTDY
                     CGVTYNSLFDSCYDTFKEANKSIANACLGPQYDSCKTDWTTTHGNPDSQGFSTFSEQA
                     TVGVSRCYRHNFGTNNAATPFMSNELSGSATNDPSGLDYPVSCSHKWPNAPGYKSGSG
                     KFTTGEEKYWKTDPTDPDTDGDGIPDGAAIIGLGKQEFTWNYQSGDRVGVAIEGTSMI
                     PTDEKSAYFKIMWGYLDTCDSTKKDLMDKDECDSSGDYGFGFLATRSPNEQGDDRLKI
                     SLSSSPENPLADPSDENKDNISSDGTIADADQITVTSSLDNTNENPTNLYYTWQISKG
                     DPQKNDWTEIKDIPGNFNTASPAAGMGISQFSFTPKKSALSSASGLNYFKVTLTASKA
                     SDLTAGRGRSSVIIPVNTQGVKISLHKVDIKDGKAVVGDEVCNDGLYKTLCPVVKGQM
                     LAAQVSGSHYKSGNSQFSWTVDGSAAYPPANASLLFDGWSNTTIFLPVTKDEQQVENI
                     SVTATSKDELQPATGTRLATVVHPVAFIKSGDTSLSWPKVYTAEDPNHKATYQNIESS
                     DA"
     gene            complement(2578..3243)
                     /locus_tag="VMQ48_00615"
     CDS             complement(2578..3243)
                     /locus_tag="VMQ48_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08575.1"
                     /translation="MKFIETKNSRKFSRRDLLIGFCAALVLAISFIFPQTAYGETFWL
                     SFFLFAIFPAAAIVFILREPLKNFGLSLGQRRPGIIFSAAIVAIFIFINYFLLFHSKY
                     GGQLSIPPFIIGSFAVFLVFEIFISLPLHFFWEFFFRGFVQLGFEKKMGIFSLPLAAA
                     AQALLSFRVNLILILLVFFSSLAAGLIVRQSRSVLYSAISLWIISASLDIMIIRIVHQ
                     VTG"
     gene            complement(3266..4066)
                     /locus_tag="VMQ48_00620"
     CDS             complement(3266..4066)
                     /locus_tag="VMQ48_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08576.1"
                     /translation="MPSQDYSGAANVNTPRLDRLREARSNYAGDTGKAAEKFGPTPRH
                     LALSEKGKLGAEKEMQRKLSSLQRNAPKTAAGLAKGKKMAVAKFAGRIFMQIDWTVDW
                     LFILLGSFALLKDIFDIVFAAAGTAGSGIWSVLVGWIPLAGPALAGAGDITMAAIGIT
                     ISFTGDLMFLILTVTVLVLVGSSLKNRGMAKYFIGIAMEFIAEALPGISWLPWTSVYV
                     FILYFCVLYDRAYGRQAQQVENPEQTENENTEPAAQIDPQASANFSKA"
     gene            complement(4081..5886)
                     /locus_tag="VMQ48_00625"
     CDS             complement(4081..5886)
                     /locus_tag="VMQ48_00625"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HUD08577.1"
                     /translation="MFGLNKNKKPAFAQSNQTDAEIEKAYHEGMASVVDLIAPAALQV
                     STSFLQIGDTFAKTIFATTYPKFLQSGWFSLIINLDRPMDIAMFIHPIESTVIMKSLR
                     RSVTQVESQIHLEQEAGKVRDPILESALENIEELRDKLQQGTERFFHFGLYITLYGRT
                     KDEIDQASQFIESLLESRMVYVKEAVFRAEQGFCSTLPLANDELSVSNNMNSSPLATT
                     FPFVSSDLSSNTGVLFGVNLHNNNLVLFDRFQMENANMVIFAKSGAGKSYAVKLEILR
                     SLMLGTEVIVIDPENEYQYLCEAVGGSFINVSLNSDYHLNPFDLPAIPEDEDPRDVLR
                     NNIASLIGLLHIMLGSITPEEDSILDRSITETYSMKDITPESNLRAFSRDQFPTMSDL
                     YEVLRSMEGAEDLATRLEKYTTGIFSGFLNNRSNVNLSSQMVVFNLRDMEEELRPIAM
                     YVVLHFIWNEIRSELKKRLIIVDEAWVMMQYEDAGSFMFGIAKRCRKYFAGLTTITQD
                     IGDFLASKYGKPIVTNSSIQLLLKQSPAAIDTIGDTFFLTDQERFMLLESNVGEGIFF
                     AGTKHVGIQVVASRSEDQLVTSNPAELLERKEKGE"
     gene            complement(6038..6733)
                     /locus_tag="VMQ48_00630"
     CDS             complement(6038..6733)
                     /locus_tag="VMQ48_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08578.1"
                     /translation="MELLHSKKLTKGEAMGKAAQAFVDVAEIKDGVVVLKNGTLRAVL
                     MVSSINFDLKSTQEQEGIVANYQNFLNSLDFPIQVVISSRKLDINPYLEMLSIKEKEQ
                     PNELLRFQIAEYRDFVRNMVDASNIMSKSFFIIIPFALSEGKKEGFVDRIRTALNPKQ
                     IMLEKKMEFENYKSQLWQRVDHVIAGLGGTGIRMAPLSTEELIELYYNAYNPGVTENT
                     DVISAEELDISRV"
     gene            complement(6735..>7103)
                     /locus_tag="VMQ48_00635"
     CDS             complement(6735..>7103)
                     /locus_tag="VMQ48_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08579.1"
                     /translation="AFGLTGKQLLWMGGGVALMVVAYSLFDRSLFFAVSFFIVLFVGA
                     LAFWRPQGVPMITFAGFTMQYFMKPRNYIWRRVYEKGNINVKKASLAQQKNNQAAFVP
                     KHLPTQGQLKKIAWELDTKK"
BASE COUNT         1719 a         1618 c         1850 g         1916 t
ORIGIN      
        1 gcatccgaac tttcaatgtt ttggtaggtt gctttgtggt ttggatcttc ggccgtatag
       61 actttcggcc agctcaagct cgtgtctccc gatttgatga aggcgactgg atgaacgacg
      121 gtcgcgaggc gcgtcccggt tgcgggctgg agctcgtctt ttgatgtggc ggtcacggag
      181 atattttcaa cttgctgctc gtcttttgtg acgggcaaaa aaatggtggt attgctccag
      241 ccgtcaaaaa gcagggaagc gtttgccgga ggataagccg cgctgccgtc aaccgtccag
      301 gagaattggc tgtttccgga tttgtagtgg cttcccgaaa cttgggcagc cagcatctga
      361 cctttcacta ccggacagag ggtcttgtag agtccgtcat tacaaacttc atcgccaaca
      421 accgcttttc catccttgat atcaactttg tggagagata tctttactcc ttgcgtgttc
      481 accggaatga tcacgcttga gcgtcccctt ccggccgtga gatcggaagc tttgcttgcc
      541 gtgagagtca ccttgaaata gttgagaccc gaggctgaag aaagagcgct ttttttcgga
      601 gtgaagctga attgggatat tcccattccg gccgcgggag aggcggtgtt gaaatttccg
      661 ggaatgtcct tgatctccgt ccagtcattt ttttgcggat ctcctttcga aatttgccag
      721 gtataataga gatttgtcgg gttttcgttt gtgttgtcaa gtgaagatgt gacggtgatc
      781 tggtcggcat ccgcaatcgt tccgtcgctc gaaatattgt ccttattctc atcgcttgga
      841 tcagctaagg gattttcagg cgaggaagaa agagaaattt tcaacctgtc atccccttgc
      901 tcgtttggtg accgggtggc aagaaatcca aagccatagt ctccggagct gtcgcattca
      961 tctttgtcca tcagatcttt tttcgtggag tcgcaggtat caagatatcc ccacataatt
     1021 ttgaagtacg cgcttttctc gtcggtcgga atcatggaag ttccctcaat ggccacaccc
     1081 actcggtcgc cggattgata gttccaggtg aattcttgtt ttccaagtcc gatgatagcc
     1141 gccccgtcgg gtatgccgtc gccgtcagtg tcagggtcag tcggatcggt cttccaatat
     1201 ttttcttcgc cggtggtgaa ttttcctgag ccggatttgt agcccggagc attcggccac
     1261 ttgtggctgc aggatacggg atagtcgagc ccggacggat cattggtagc gctgccggag
     1321 agttcattcg acataaaagg cgtagcggcg ttgttggtgc cgaaattgtg gcgatagcag
     1381 cgggaaacgc ccacggtggc ttgttcactg aaagtggaaa aaccctggct gtccggattt
     1441 ccgtgagttg tcgtccaatc tgttttgcag gaatcatatt gcggaccaag gcaggcattg
     1501 gcgatgcttt tgttggcttc cttgaaagta tcgtaacaac tgtcaaaaag tgagttgtag
     1561 gtcactccgc aatagtcggt gccgctgctt tgcgggcatt ttgaataggt cgtcacgtcg
     1621 gacaccgctc cggtatccgg atcggtcact tgggtggtcg tggaacattg aagggatgat
     1681 tgcggcgtat ttgcgtagca gcaattgttc cgagttgttt ttgcgcttgc gaaagcagtt
     1741 gctccgtctc ccgagaaata atctttgcaa aggtcgcaat aatgattgtg ctgggatgat
     1801 ttcagtgtga agtacgagtc cagcggcctt gaagaagtat cgtcattata tgagcagttg
     1861 tcccaagcgt ggccgccgag agaatcgcac cagtcggtgg aggttttata tgaatcaaca
     1921 gtctggtcgg ccaatcctcc gactccgtcc gatcctccca tcggtgcggc gcaagtattc
     1981 gaatcatagc tattggtatc cacagccggc cagccgtcgt tgtccggatc ttttcccgga
     2041 tcggcgtagg tttgcccgtc aagatttgga tcatagtctc cccgagccat gattccggcg
     2101 gcttcggtct tcccttcttt gatgctgttg gtggctgttt ggatccgtcc gtccgttgtg
     2161 tgcatgatat accaggtata atagaggttc tgcgggtcgt ttttgaaaaa ttccggaacg
     2221 gcgtgggcgg tcactttttc gccaactttc gggtttgtat tgtcgaaaaa cacttccgcg
     2281 cggggagcgt ctgctttgcg ttcggccgaa cgaaaaatat cactgctgat cccatagcgt
     2341 tgttcggtgg agttggtaat gccggttgcc gcattttgag aaatttgaga tattgatgag
     2401 tactgcgctt cctggagaat ttggttgacg tcgatgttga cgcttcctcc attggacaaa
     2461 ctgggaagtc cgaccgtgta ggcctgggcg gcaaaaggca aaacaagatt cagcgtgaga
     2521 gcaaaaactg aaaacatgga gacaattatt tttttgtttt ttttcatttt catatattta
     2581 gccggtcacc tgatggacta ttcttattat cattatatcc aagctggccg aaattatcca
     2641 cagagatatg gcggagtata aaacagaccg gctttggcgc acgatgagtc ccgcggcgag
     2701 cgaagaaaag aaaaccagca aaatcaaaat caaattgacc cggaaagaca gcagcgcttg
     2761 agcagcggcg gctaagggca aggaaaatat tcccattttt ttttcaaatc ccaattgtac
     2821 gaatccccgg aagaaaaatt cccagaaaaa atgaagcgga agggaaatga atatctcaaa
     2881 aacgagaaag accgcgaaac tgccgataat gaagggagga atggaaagct gtccgccgta
     2941 tttggaatga aaaagaagaa aatagttgat gaaaataaaa atagcgacaa tagctgcaga
     3001 gaagataatg ccgggacgcc gctgtccaag ggaaaggccg aaatttttga ggggttctct
     3061 gagaataaac acgatggcgg cagccggaaa gatcgcaaaa agaaaaaaag agagccaaaa
     3121 tgtttctcca taagcggttt gaggaaaaat aaagctgata gcaagaacaa gcgcggcgca
     3181 aaaaccaatc aggagatccc gccgggagaa ctttcggctg tttttagttt cgatgaattt
     3241 catggccata aaatttgttc taaggctaag ccttagaaaa gttagcgctt gcttgtggat
     3301 cgatttgggc ggcaggttcg gtgttttcgt tttctgtctg ctcgggattt tcaacttgct
     3361 gggcttgcct gccgtatgct ctgtcataga gaacgcagaa atagaggata aagacatata
     3421 cggacgtcca cggcagccaa ctgatgccag gtagcgcttc ggcgatgaat tccattgcta
     3481 ttccgatgaa atattttgcc atccccctgt ttttgaggct tgatcctacc aaaaccagaa
     3541 cagtgacagt caaaatgaga aacataaggt ctccggtaaa actgatggtt attccgatag
     3601 cggccattgt aatgtcgcct gctcctgcta gtgcaggacc ggccaatggt atccagccga
     3661 ccaacacact ccatattccg ctgccggctg tccctgcggc ggcgaaaaca atatcaaaaa
     3721 tatctttcaa caaagcgaac gaacccagaa gaataaacaa ccaatcgaca gtccaatcaa
     3781 tttgcatgaa aattcttccc gcgaatttcg cgacagccat tttctttccc ttcgcgaggc
     3841 cggcggccgt tttcggagcg tttctttgca agctggaaag tttgcgctgc atttcctttt
     3901 ctgctcccaa ttttcccttt tccgataaag ccaaatgtct tggtgtcgga ccgaatttct
     3961 cagcagcttt tccggtatcg cctgcatagt tagaccgagc ttctctgagc ctatctaacc
     4021 ttggagtatt gacattggct gcgccgctgt aatcttgcga tggcattgat tttatttata
     4081 ttattcccct ttctcttttc tttccaaaag ctcggcgggg ttcgaagtga cgagttggtc
     4141 ttccgagcgg gaggcgacaa cctggattcc gacgtgcttg gtgccggcga agaatattcc
     4201 ttctccgaca tttgattcga gcagcataaa tctttcctgg tcagtgagga aaaaagtgtc
     4261 tccaatcgtg tcgatcgcag ccggcgactg cttgaggaga agctggattg aggagttggt
     4321 gacgatcggt ttgccgtatt tcgaagcgag gaaatcgccg atatcctgcg tgattgtggt
     4381 gaggccggcg aaatatttgc ggcaacgttt ggcgattcca aacatgaacg atcctgcgtc
     4441 ctcatattgc atcataaccc aggcttcatc gacgatgatt aacctttttt tgagctccga
     4501 gcggatttca ttccagatga agtgcaaaac aacatacatt gcgatcgggc gcagctcctc
     4561 ttccatgtcg cgaagattga aaacaaccat ctggctggaa aggttcacat tcgagcggtt
     4621 gttgagaaat ccggaaaaaa ttcccgtcgt atatttttcg agccgcgtgg ccaggtcctc
     4681 cgccccctcc atgctgcgca gaacttcata gaggtccgac atcgtcggga attggtcgcg
     4741 tgaaaacgcg cgcaagttgc tttcaggagt aatgtctttc atcgagtatg tttccgtgat
     4801 cgaccggtcg aggatcgagt cttcttccgg cgtgatgctt ccgagcatga tgtggagcag
     4861 cccgatgagg ctggcgatat tgttgcgcaa aacgtcgcgg ggatcttcat cttcgggaat
     4921 ggcgggcaaa tcaaaagggt tgaggtgata gtcggagttg agcgaaacat tgatgaaact
     4981 tccgccgaca gcttcgcaca aatattggta ttcgttttcc ggatcaatga cgatcacttc
     5041 cgtcccgagc atcagggagc ggaggatctc tagtttcacg gcatagcttt ttccggcgcc
     5101 ggacttggcg aagatcacca tattggcgtt ttccatctgg aagcggtcga aaaggacgag
     5161 gttgttgttg tgcaaattca cgccaaagag gacgccggtg ttcgaagaaa gatcagacga
     5221 aacgaacgga aaagtggtcg cgagcggcga gctgttcata ttgttcgaga cggaaagttc
     5281 gtcgttggcg agcggaagag tcgagcaaaa accttgttcg gcacggaaaa cggcctcttt
     5341 cacatagacc atccgggatt caagcagaga ttcgatgaat tgggaagcct ggtcaatctc
     5401 gtctttcgtc cggccataaa gcgtgatata caagccaaaa tggaagaaac gctctgttcc
     5461 ttgttgcagt ttgtcgcgca attcctcaat gttttcgagc gccgattcaa ggatcggatc
     5521 gcgcactttt ccggcttcct gttcaagatg gatctgggac tccacctggg tgaccgagcg
     5581 gcgcaggctt ttcatgatca cagtcgattc gatcggatgg atgaacatcg cgatatccat
     5641 cggtcggtcc aaattgatga tcagcgaaaa ccagcctgac tggagaaatt ttggataggt
     5701 ggttgcgaaa atagttttgg caaaggtatc gccgatttgg agaaaagaag tcgaaacctg
     5761 aagggcggcg ggagcgataa gatcgaccac ggaagccatg ccctcgtgat aggccttttc
     5821 gatttcagca tccgtttgat tggattgcgc gaaggcaggt tttttgtttt tattcagtcc
     5881 aaacataaaa atttctaatt tctaattgat ctaattgacc taaaaaaatc ccaaaattca
     5941 aatcccaaaa ctattttatc ttctagcgaa atatcatctt tggacattgg ggtttgggat
     6001 tttcttagat taattagaaa ttaggtcaat tagtaattta aacccggctt atatccaact
     6061 cttccgcgct tatcacatcc gtattctccg tcactcccgg attgtaggcg ttgtaataga
     6121 gttcaatcag ctcttcggtc gaaagcggcg ccatacggat gcctgttcct cccagcccgg
     6181 cgatgacgtg gtcgactctc tgccaaagct ggcttttgta attttcaaat tccatttttt
     6241 tctcgagcat gatctgcttg gggttgagtg ccgtgcggat acggtcgacg aagccttctt
     6301 tcttgccttc cgacagggcg aaagggatga tgatgaagaa cgacttggac atgatatttg
     6361 aagcgtccac catgtttctc acgaaatccc ggtactcggc gatctgaaag cgcagaagtt
     6421 cgttcggttg ttctttttcc ttgatggaaa gcatttcaag atacggattg atgtcgagtt
     6481 ttcgcgaact gatcacgacc tggatcggaa aatcaagcga gttcaaaaaa ttctggtagt
     6541 tcgcgacgat gccctcttgt tcctgggttg atttgagatc gaagtttatt gaagaaacca
     6601 tcaaaaccgc ccggagtgtc ccgttcttca gcaccacgac tccgtctttg atttccgcga
     6661 cgtcgacaaa agcttgggca gctttgccca tcgcctcgcc tttggtcagt ttttttgagt
     6721 gtaataattc catattattt ttttgtatca agttcccacg cgattttctt gagttgtccc
     6781 tgggttggca gatgcttggg aacaaaagcc gcctggttgt ttttctgttg ggcgagggac
     6841 gcttttttga cgttgatatt tcctttttca tacactcttc tccagatata gtttcggggt
     6901 ttcatgaaat attgcatagt gaatccggcg aaagtgatca tggggactcc ttgcggcctc
     6961 caaaaggcga gtgcaccgac gaaaaggacg atgaaaaatg agacggcgaa aaacaaagag
     7021 cggtcgaaga ggctataggc gacaaccatg agtgccacgc ccccgcccat ccacaaaagc
     7081 tgcttgccgg tgagcccgaa agc
//
LOCUS       DATKGS010000026         2619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_146739, whole genome shotgun sequence.
ACCESSION   DATKGS010000026 DATKGS010000000
VERSION     DATKGS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146739"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VMQ48_00640"
     CDS             complement(<1..142)
                     /locus_tag="VMQ48_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HUD08580.1"
                     /translation="MFIGEYNHNIDPKKRVAIPAKFRNDFSGKAVVTRGLDKCLFIYP
                     LIS"
     gene            complement(445..1260)
                     /locus_tag="VMQ48_00645"
     CDS             complement(445..1260)
                     /locus_tag="VMQ48_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HUD08581.1"
                     /translation="MRKQKIEVRGIEIAIITKEDQDYISLTDMLRAKDGDFFISDWLR
                     NRNTIEFLGIWEKINNPNFNYGEFAIIKSQAGLNSYKISVKEWVKKTNAIGLKAAAGR
                     YGGTYAHKDIAFEFGMWVSPEFKIYLIKEFQRLKIEENEKLVLGWDAKRMLTKINYRI
                     HTDAIKENIIIPLKLSEKEANIVFANEADVLNMALFGMTAKQWRDKNPKLAGNIRDYA
                     DVAQLVCLANLENLNAEFIRVSLSQKKRLLKLNEIAIIQMKSLANNPSIKKLE"
     gene            complement(1536..>2619)
                     /locus_tag="VMQ48_00650"
     CDS             complement(1536..>2619)
                     /locus_tag="VMQ48_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08582.1"
                     /translation="WFEEEEDVAWRAWVDFKKREVGDLIGLLTADVRDSLKKRALFLL
                     VVPSADFNPIYWTEEVGKYFRRLDYLKRLTPDLLAFVAELVTKFCDILRPKHCGRPQT
                     VVEGDENFTAYMLVPDKYHDALEFYNKCILMLIQLLPLEQAEKIFPFYSLQDVSSFWS
                     PDSATGYRPFDFIMRSNIDEKWKMLADAAMRKIILEEIAGQTKPREDWENALSCYANF
                     AALHLFGKLPYSLELFAEQIQFIMDNRQASEHDLIPDYQVIKIFNLLPGDAYAELRHA
                     IARYAVVEDRKDEHFRFLMYHEDQAVDWIAEEFGGSDPELADSLKALLKEGDAQQRER
                     DAQQEVVNAQRKKMRETEENILAQMK"
BASE COUNT          692 a          637 c          469 g          821 t
ORIGIN      
        1 gggagatgag cggatatata aaaaggcatt tgtcgagtcc gcgtgtcaca accgcttttc
       61 cgctaaaatc atttcgaaat ttcgccggaa tagcgactct cttctttgga tcaatattat
      121 ggttatactc gcctataaac ataaatctgc ttgctaaaaa atactcgcaa ctaagttcat
      181 tcgtatattc gtatggattc gtaattcgta gagaaaagaa tgtgatctat gccgagttgt
      241 ccacaatctt ggtcagttat ccccacttcc atccactttc caacactgta catacattct
      301 aatccactcc gaaacatcgg tcaacacttt gcggacaaaa taaaaatggc ttatacaagc
      361 cactggaaag cattttcccc acaggttatc cactttttgt ttaatgttag gacggtctat
      421 gttatttgat ttatgtattc atgattattc cagttttttg atgcttggat tattggccaa
      481 tgatttcatc tggatgattg caatctcatt taattttagc aatcgctttt tctgggataa
      541 gctaacccga atgaactccg cattgagatt ttccaaattt gctagacaca cgagttgagc
      601 cacatctgcg taatcacgaa tatttcccgc cagcttcgga tttttatctc tccattgttt
      661 tgctgtcatt ccaaaaaggg ccatattcaa aacatccgcc tcatttgcaa aaacaatatt
      721 cgcttctttt tccgacaatt tcaaaggaat aatgatgttt tcctttattg catcagtgtg
      781 aattctataa ttgatttttg tcagcattct tttggcgtcc catcccagca ccagcttttc
      841 attttcttca attttgagcc tctgaaattc tttgattaga taaattttga attccggact
      901 gacccacatt ccaaactcaa aagcaatatc cttatgagca tatgttccgc catatcgacc
      961 ggccgcggct ttcaatccaa ttgcgtttgt ctttttcacc cattctttta cgcttatttt
     1021 gtagctattt aaacccgcct gacttttaat tatggcaaat tcgccataat taaaatttgg
     1081 gttgtttatc ttctcccaaa ttccgagaaa ttcaattgta ttcctgtttc tcagccaatc
     1141 ggaaatgaaa aaatcgccat ccttcgcccg caacatatcc gtaagcgaaa tataatcctg
     1201 atcttctttg gttatgatgg ctatttcgat tcctctcact tcaatttttt gttttctcat
     1261 aatgatcttt tatttttatg gctatttttt tctaaagttt aacttagtgt aattttacaa
     1321 tttatcttcc gagattattt tatcacaaat tttcacacaa aaaaatcccg cccacttcgc
     1381 agggcggagg cgcaggatgc ttattttcta ttttcctaac atttttccta acattgaatg
     1441 ttaggaaaga ttaaacttta ggaagtttta aaaaatgttg tgagaaaaaa tgaagccctc
     1501 ggcaattaat accaagggct ccgtggttga agcctttact tcatctgggc gaggatgttc
     1561 tcctccgttt ctctcatttt ttttctttga gcattcacta cttcttgttg agcatcgcgc
     1621 tctctttgct gcgcgtcacc ctccttaagc aacgccttaa gggaatcagc gagctcagga
     1681 tcgctccccc cgaattcttc cgcgatccag tcgaccgcct gatcttcatg atacatcaaa
     1741 aaacggaagt gctcatcctt cctatcttcc accacggcat atctggcaat cgcatgccga
     1801 agttcggcat acgcatcgcc cggaagaaga ttgaaaatct tgatgacctg ataatcggga
     1861 attaagtcat gctcactcgc ctggcgattg tccatgatga attggatttg ctccgcaaac
     1921 agctccaatg aatacggaag cttcccaaag agatgcaatg cggcaaagtt cgcatagcag
     1981 ctcaacgcgt tctcccaatc ctccctgggc ttcgtttgcc cggcaatttc ttccaggatg
     2041 attttccgca tcgcggcgtc ggccagcatc ttccactttt catcgatatt actgcgcatg
     2101 atgaaatcaa atggtctgta gccggtagca ctgtccggac tccaaaaaga tgaaacatcc
     2161 tgaagagaat agaacgggaa aattttctcc gcttgctcca gaggaagcag ttggatcagc
     2221 atcaagatgc atttgttgta gaattccagc gcatcatgat atttgtccgg aaccaacata
     2281 taggccgtga aattttcgtc accctcaacg acagtttgcg gccggccaca gtgctttggt
     2341 ctcaagatat cgcagaactt ggtcacgagt tcagccacaa aggccagaag atccggggtt
     2401 agccgcttca gatagtccaa acggcggaaa tatttgccca cctcttccgt ccaatagatc
     2461 ggattgaaat ccgccgaggg aacaacaagc agaaataacg cccgcttctt gaggctgtcc
     2521 ctcacatcag cagttaagag gccgatcaaa tctcccactt ctctcttctt gaagtctacc
     2581 cacgccctcc atgcgacgtc ctcttcctct tcaaaccac
//
LOCUS       DATKGS010000027         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_175440, whole genome shotgun sequence.
ACCESSION   DATKGS010000027 DATKGS010000000
VERSION     DATKGS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175440"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..358)
                     /locus_tag="VMQ48_00655"
     CDS             complement(50..358)
                     /locus_tag="VMQ48_00655"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HUD08583.1"
                     /translation="MSKARAKKIVKKYAEKLKAEKFPFSAIWLFGSMAKGKAHKWSDI
                     DVAVVSKKLSSDKEESKLWEYRKDVSDLIEPHGFTPKDFADYWNPMAHEVKKTGIRVA
                     "
     gene            complement(331..738)
                     /locus_tag="VMQ48_00660"
     CDS             complement(331..738)
                     /locus_tag="VMQ48_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HUD08584.1"
                     /translation="MKKTGYKKLVEYWQKTAEYDYDTMKSLLKSKRYASSLFFGHIVL
                     EKILKALVVNNTKDHAPYIHDLIRLFQLAGLEIDKDTRKFLDEVNKFNIKARYPEQKL
                     NFYKKCTKEYCGKRINDIDTLYKKLCRKLEPKK"
     gene            1056..2063
                     /gene="ruvB"
                     /locus_tag="VMQ48_00665"
     CDS             1056..2063
                     /gene="ruvB"
                     /locus_tag="VMQ48_00665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HUD08585.1"
                     /translation="MITSSNEQTDDTKLDITLRPQSFGEYFGQEKTKKNLRILIDAAK
                     KRKEPIEHVLLYGPAGLGKTTLAHIIANEMGANIRVTSGPAIEKVGDLGSILTNLGDG
                     DILFIDEIHRLNKLIEEVLYPAMEDFKLDIIIGKGPSAKTLQLDLPRFTLIGATTRLG
                     AISSPLRNRFGVINRLDFYEPSEIESIVHRSSKILSAPIDSAGAKTVSQSSRRTPRVA
                     NRLLKRVRDFTQVNDSDTITEELCRAAFANLEIDELGLEPTDRQILRTIIEKFNGGPV
                     GISTIAAATSEEIETIEDVYEPFLLQIGFLTRTPRGRMATEAAYKHLGIKYDKEQDKL
                     F"
     gene            2102..2623
                     /locus_tag="VMQ48_00670"
     CDS             2102..2623
                     /locus_tag="VMQ48_00670"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HUD08586.1"
                     /translation="MKKEITIPQETIRQKIYLIRDEKVMFDRDLAELYDVETRILNQA
                     VKRNKDRFPSDFMFQLTKQEMEIWISQIVISNREKMGLRKPPLVFTEQGVAMLSSVLN
                     SKRAIHVNIQIIRTFTMLRKILATHKELRKKIDAMEQKYDKRFKVIFDTLRLLIKEDK
                     KPKKPIGFYDRKP"
     gene            2662..>3066
                     /locus_tag="VMQ48_00675"
     CDS             2662..>3066
                     /locus_tag="VMQ48_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08587.1"
                     /translation="MTLRKGILWSQKKMRPQNVKRSGKYQGVMGKLMSNEKIAKALDR
                     PGERKEFYGMLKSVSEGGVYKNELRKFFGDLRSGKVKTRTINAKELRVIAKEIFPDSA
                     KRYTFKESKSQKNTATPSQNAAMFDEIRRGFSP"
BASE COUNT          934 a          706 c          631 g          795 t
ORIGIN      
        1 ggataaaaaa agacgcccca gcaggacgtc tctactcttt tgacacattc tacgcaaccc
       61 tgattcctgt ttttttgact tcgtgtgcca ttggattcca ataatcggcg aaatctttcg
      121 gggtgaaacc gtgcggctcg atcaaatcag aaacatcttt gcgatattcc cacaattttg
      181 actcttcttt gtccgagctt aattttttgg acacaaccgc cacatcgatg tcgctccatt
      241 tgtgcgcttt gcccttcgcc attgaaccga acaaccaaat cgcggaaaaa ggaaactttt
      301 ccgctttcag tttttcagcg tattttttga ctattttttt ggctctagct ttcgacataa
      361 ttttttatac aaagtgtcaa tatcatttat acgttttccg caatattctt ttgtgcattt
      421 tttataaaaa tttaatttct gttccggata acgggctttt atgttgaatt tattcacctc
      481 atccaaaaac tttcttgtat ctttgtctat ttccaaacca gcaagctgaa atagtctaat
      541 caaatcgtga atatacggcg cgtggtcttt cgtattgtta acgaccagcg ctttcagaat
      601 tttttccaag acgatatggc caaaaaataa gctgctggcg taacgcttgc ttttcaaaag
      661 gcttttcatc gtgtcataat cgtattctgc cgttttttgc caatattcaa ccaatttttt
      721 gtatccggtc tttttcatgc tttcatttta ttacgctttt gagtttcagt caattttgag
      781 aaaacgaaaa aaccctctga aatgcaggca aattcccgca actagggaat cgcggggtag
      841 gaacagtgcc gaggaagagt tcagccgaaa ccgaacaatc cgtgtctggg caaaaaagtt
      901 cccggtagga aagttacgag ctccccttat tatgcctgca aatcagaagg ttctggtgga
      961 aattgtgctt ttttatgcgc tactttcagg attttacagc ttatcacagg ctgatttttt
     1021 tgtcaagcag accaaaataa gctatactgg cgataatgat tacctcttcc aacgagcaaa
     1081 ccgacgatac aaagctggat atcacccttc gcccgcaaag tttcggggaa tattttgggc
     1141 aggaaaaaac caagaaaaat ctacggattt tgatcgacgc cgccaaaaaa agaaaagagc
     1201 cgattgagca tgttttgctc tatggcccgg cgggactcgg aaaaacaacg cttgcccaca
     1261 tcattgccaa tgaaatgggc gccaatatcc gcgtcacctc cggcccggcc attgaaaaag
     1321 tgggcgatct gggatcaatt ctcaccaatc tcggcgacgg cgacatcctt ttcattgacg
     1381 aaatccaccg cctcaacaaa ttgattgagg aagttctcta tcccgccatg gaagatttca
     1441 aactcgatat catcatcggc aaaggcccgt cggcaaaaac tttgcaattg gatctccccc
     1501 gctttacttt gataggagct actacgcgct tgggggcgat ttcttccccg cttcgcaacc
     1561 gtttcggcgt catcaatcgg ctcgatttct atgaacccag cgagattgaa agtatagtcc
     1621 accgctcctc gaaaatcctt tccgctccaa ttgacagcgc cggagcaaaa actgtttccc
     1681 aatccagccg ccgcaccccg cgggtcgcca atcggctctt gaaacgcgtg cgtgatttca
     1741 cgcaggtgaa tgacagcgac acgatcacgg aagaactttg ccgcgcggcg tttgccaatc
     1801 ttgagatcga tgaattggga ctcgagccca cagacagaca aatcttgcgg acaataatcg
     1861 aaaaattcaa cggcggaccg gtcggcattt ccaccatcgc cgccgcaact tcggaggaaa
     1921 ttgaaacaat cgaggatgtc tatgaaccct ttcttctcca aattggattc ctcacccgta
     1981 ccccccgagg ccggatggca acggaagcgg catacaaaca tttagggata aagtatgaca
     2041 aagaacagga taagttattt tgatcctaag gcgccaactt ggtaccataa gaaaaacaaa
     2101 aatgaaaaaa gaaattacaa ttccgcaaga aacaattcga caaaaaatat atctgattag
     2161 ggatgaaaag gttatgtttg accgcgattt ggcggaatta tacgacgtgg aaacaagaat
     2221 tctgaatcaa gcagtcaaac gaaataaaga tcgttttcct tccgatttta tgtttcaact
     2281 cacaaagcaa gaaatggaaa tttggatatc acaaattgtg atatccaatc gcgagaaaat
     2341 ggggctaaga aaaccgcccc ttgttttcac cgaacaaggt gtcgctatgc tctcaagcgt
     2401 gctcaatagc aaaagagcca tccacgtcaa tattcaaatc atacggactt ttacgatgct
     2461 ccgaaaaatt cttgctactc acaaagaatt gcggaaaaaa atcgacgcaa tggagcaaaa
     2521 atatgacaag cgattcaaag ttatattcga cacattgcgt ttgctgatca aagaagacaa
     2581 gaaaccgaaa aaaccaatcg gcttttatga tcgaaagcct taacgtataa taaggagcag
     2641 gataagttgt tctaaaaaca tatgacactt cgaaaaggca tcctttggtc gcagaaaaaa
     2701 atgcgtccgc aaaatgtgaa gcgcagcggc aaatatcagg gcgtgatggg aaagttgatg
     2761 agcaatgaaa agattgccaa agctcttgac aggccgggtg aaagaaaaga attctatgga
     2821 atgctcaaga gcgtgtccga gggtggggtt tataaaaatg aactgcgcaa attttttggc
     2881 gatctccggt caggcaaagt caaaacccgc accatcaatg caaaagaact ccgcgtaatc
     2941 gccaaagaga tttttccgga cagcgcgaag agatatacat tcaaagaatc gaaatcccaa
     3001 aaaaataccg ccacccccag ccaaaatgcc gcaatgtttg atgaaataag aagaggattc
     3061 tctccc
//
LOCUS       DATKGS010000028         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_3133, whole genome shotgun sequence.
ACCESSION   DATKGS010000028 DATKGS010000000
VERSION     DATKGS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3133"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>2662
                     /gene="uvrA"
                     /locus_tag="VMQ48_00680"
     CDS             29..>2662
                     /gene="uvrA"
                     /locus_tag="VMQ48_00680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUD08588.1"
                     /translation="MDKIVIRGAREHNLKNINLELPRGKFIVFTGISGSGKSTLAFDT
                     IFAEGQRRYLESLSSYARQFLGQMEKPDVDSIEGLSPAISIDQKASSHNPRSTVGTVT
                     EIHDYLRLLYAKIGRPHCPVCGKEITKMSIDEIVDRIIDCRRPTSTKIEILSPVVRER
                     KGDYATLLAEMWKRGFAEAYVDGKRVILEDEAKKGKILARYKKHSIDILIDTVEVSDK
                     NISRIFESTEGALNLSKGFVKVRSGKSEHIFNQNMACLDHEEAVFPELEPRLFSFNSP
                     QGACPACEGLGTKKEIDPNLIIPDKTRTIAEGAIMPWSYKPNNYQGSILRAVTNYYRI
                     PDNVRFRDLSEKQQNILLFGDEEPDDIEVRLKTKTGAVWRFNVEWNGILGFMRDRYFK
                     TESEAVRQDIEKYMSQSPCSACKGSRYKPEALLITVGNKNISEISTVSVNETLKFFEG
                     IELNEREKIIADKILKEVKNRLKFLDDVGLGYLTLGRSANTLAGGETQRIRLASQIGS
                     QLVGVLYILDEPSVGLHARDNKKLLETLLKLRDIGNTVIVIEHDEETIRAADHLVDIG
                     PGAGRLGGEIVAEGTVKDIEKAKDSITGKYLRGDSKIEIPKFRRPVRNKKWLTVKGAS
                     EHNLKSITVEFPLKTLTCVTGVSGSGKSTLVEDILGKGLSSKIMRSLERPGRHKEILG
                     TQYVDKVIHIDQSPIGRTPRSNPATYVGFFTLIRELFAMTKDARGHGYGPGRFSFNVN
                     GGRCDNCRGEGFLKVEMQFMPDVFLPCDVCKGKRYNRETLEVKYKGKNIAEVLAMTVS
                     EGKDFFENYPRIFEPLKVLEEVGLGYINLGQAATTLSGGEAQRIKLASELSRRMTGNT
                     VYILDEPTTGLHFDDVKKLLEV"
BASE COUNT          774 a          543 c          742 g          603 t
ORIGIN      
        1 gcatttttgt gttaggaagc aataatatat ggataaaata gttattcgcg gggcgcgcga
       61 gcataatttg aagaatataa acctcgaact tccgcggggg aagttcatcg tgttcacggg
      121 gatcagcggg tcgggaaaat caactctcgc ttttgatacc atctttgccg agggacaaag
      181 gcgatatttg gagagtttgt cgagttatgc ccggcagttt ttgggacaga tggaaaaacc
      241 ggacgtggat tcgatcgaag gcttgagccc ggcgatttcg atcgaccaaa aagcgtcgtc
      301 gcacaatccg cgctcaacgg tcggaacggt gacggaaatt catgattatc tgcggctttt
      361 atacgccaaa ataggccggc cgcattgccc ggtgtgcgga aaagagatca cgaaaatgtc
      421 gattgatgaa atcgtggatc gaataatcga ttgtagacgt ccgacgtcta caaaaatcga
      481 aatattatcg cccgtggtgc gcgaacgaaa gggggattat gcgacgctgc tggcggaaat
      541 gtggaagcgc ggatttgccg aggcttatgt tgatgggaaa agagtgatcc ttgaagatga
      601 agccaaaaaa ggaaagatat tggccagata caaaaaacac agtatcgata ttttaattga
      661 tacggtggaa gtaagcgaca aaaatatcag ccggattttt gaaagcacgg aaggcgcgct
      721 aaatttatca aaaggatttg taaaagtgcg tagcggaaag tccgagcata ttttcaatca
      781 aaatatggct tgtctggatc atgaagaggc tgttttcccg gagcttgagc cgcgtttatt
      841 ttctttcaat agccctcaag gagcttgccc cgcttgtgaa ggtttgggga cgaaaaaaga
      901 aattgatcca aacctcatca ttcccgacaa gacgcggacg attgccgaag gagcgattat
      961 gccatggagt tacaaaccga ataattatca gggttcgatc ctgcgcgccg tgacaaacta
     1021 ttatcgcatt ccggacaatg tccgcttccg ggatctctcc gaaaagcaac aaaatatttt
     1081 gctttttggc gatgaagagc ccgatgatat cgaagttcgc ctcaaaacaa agaccggagc
     1141 ggtctggcgc ttcaacgtcg agtggaacgg tatcttggga tttatgcgag accgctattt
     1201 caaaaccgaa tcggaagcgg tgcgtcagga catcgaaaaa tatatgtccc aaagtccgtg
     1261 ttcggcctgc aaaggctcgc gatacaagcc ggaagcgctg cttatcactg tgggaaacaa
     1321 aaatatctct gaaatttcaa ctgtgagcgt gaatgagaca ctgaaatttt tcgagggtat
     1381 cgaattgaat gagcgggaaa aaataatcgc cgacaaaatc ctgaaagaag tgaaaaatcg
     1441 tctgaagttt ctggatgacg tgggattggg atatttgacg ctgggacgtt cggccaacac
     1501 attggccggc ggtgaaacgc aaagaatccg tctggcgtca caaatcggct cgcaattggt
     1561 gggtgtatta tacattctcg acgagccgtc tgttggcttg cacgcgagag ataataagaa
     1621 attacttgag acattgctga aactccgaga tattggcaac actgtgattg tcattgaaca
     1681 tgacgaagaa acgatccggg cggccgatca tttggtggat atcggcccgg gagccgggcg
     1741 tcttggcggg gaaattgtgg cggaagggac agtgaaggat attgaaaaag cgaaagattc
     1801 catcacggga aaatatttgc gcggagactc aaaaatagaa attcccaaat tccgccggcc
     1861 ggttcggaac aaaaaatggc tcaccgtgaa gggagccagc gaacacaatc tcaagagcat
     1921 aaccgtcgaa tttcctttga aaacattgac ttgcgtgaca ggtgtcagcg gcagcggaaa
     1981 gtcgacgctg gtcgaagata ttctgggaaa aggcctatcc tcaaaaatta tgcgcagtct
     2041 cgaaaggccg gggcggcaca aggaaattct tgggacgcaa tacgtcgaca aagtgatcca
     2101 catcgaccag tcgccgatag ggcgcacgcc aaggtcgaat cccgcgacct atgtcggatt
     2161 tttcaccttg atccgcgaac tttttgcgat gaccaaagac gcgaggggac acggatatgg
     2221 tccgggaaga ttttcgttca acgtgaacgg gggacggtgc gacaattgcc gaggcgaagg
     2281 ttttttgaaa gtggaaatgc aatttatgcc ggacgtgttt ttgccgtgcg atgtttgcaa
     2341 agggaaaaga tataacaggg aaacgctcga ggtgaaatat aaagggaaaa atatcgccga
     2401 ggtgctcgcg atgacggttt cggaagggaa ggattttttt gaaaactatc cgcgaatctt
     2461 cgaaccgctc aaagtcttgg aagaagtggg gctgggatac atcaatttgg gtcaagcggc
     2521 gacgacgctt tcgggcggag aagcgcagcg gataaaactg gcgtcggaac tttcgcgccg
     2581 gatgacgggc aatacagtat acattctcga cgagccgaca acgggtctcc atttcgatga
     2641 cgtgaaaaaa ctcttggaag tt
//
LOCUS       DATKGS010000029         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_175579, whole genome shotgun sequence.
ACCESSION   DATKGS010000029 DATKGS010000000
VERSION     DATKGS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175579"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /gene="pheS"
                     /locus_tag="VMQ48_00685"
     CDS             <1..1026
                     /gene="pheS"
                     /locus_tag="VMQ48_00685"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HUD08589.1"
                     /translation="HMLQKKIEELKAKAISDIKKAADERALFDVKVKYLGRKGKLTEI
                     LKSLKNLSESERKVVGPLANEARFEIEKAITTRKKEISEKIDWEKEKIDITLPGKKQE
                     LGHLSPITLVRRDIENIFSTMGFEIADGPELEFEKYNFDALNFPKDHPARDMQDTFWI
                     KSQKEKLLLRTHTSPVQIRYMEKHKPPFQVIIPGRVFRNEATDAKHEHTLHQFEALVV
                     GDNINVGNFKYLAKQFFSAFFGIKTDIRLRPSFFPFTEPSFEFDITCAICSGKGCSTC
                     KNAGWIEIGGAGMVNQNVFIASGYPRNKYQGFAWGFGLERLAMMKYKIDDIRWIESGD
                     LRFIKQF"
     gene            1028..>2122
                     /locus_tag="VMQ48_00690"
     CDS             1028..>2122
                     /locus_tag="VMQ48_00690"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HUD08590.1"
                     /translation="MKYSYNWLKELSGTKLSPEKLSDRLMMRSFEFEEMKKEGKETQL
                     EFAILPNRAHDMLSHIGMAREICAVEGRKFKHEIAAVATLPRNDKIRVEIKDKKLCRR
                     YIGAVLENIKVAPSPKWIQERLLVSGVKPINNIVDITNYVMLETGQSLHAFDAKKTTG
                     NIIVRKAKKGEKIKLLDEKEYELGESDLVIADSEKALALAGVMGGAESSVASKTTSII
                     LESANFNSTNIRKTRTRHNIITESSYRFERDIDPNLAEIGAARAIELLQKYGGSNAKL
                     VAYTDIYPKKLKPWQIKLNADYVNSLLGEKVPVSRIKNILENLGLGVKISKNILNCEI
                     PTVRIDLKTQEDLIEEIGRIYGYENILAKAP"
BASE COUNT          701 a          389 c          497 g          535 t
ORIGIN      
        1 catatgctgc agaagaaaat cgaagaactg aaagcgaaag ccatttccga tatcaagaaa
       61 gccgcggatg aacgggcgct ttttgatgtg aaggtgaagt atctgggccg aaagggaaaa
      121 ttgacggaaa ttttgaaaag cctaaagaat ttgagtgaaa gtgagagaaa agttgtcggg
      181 ccgctagcca acgaagcaag atttgaaatt gaaaaagcga ttacaactcg caaaaaagaa
      241 atttcggaaa aaattgattg ggagaaagaa aaaattgata tcactctccc cggaaaaaaa
      301 caagaactcg gacatttgag cccgattact ttggtcaggc gtgatatcga aaatattttt
      361 tccacgatgg gttttgagat cgctgatggc ccggaattgg aatttgaaaa atacaacttt
      421 gatgctttga attttcccaa agaccacccg gcacgcgata tgcaggatac tttttggatc
      481 aagtcgcaaa aagaaaaact tctcttgaga acccatactt ctccggtgca aatacgatat
      541 atggaaaaac ataagccgcc ttttcaggtg ataattccgg gtcgagtttt tcggaatgaa
      601 gcgacggacg cgaaacacga acatacattg catcaatttg aagctttggt tgttggcgat
      661 aatataaatg tcggaaattt caaatattta gccaagcaat ttttcagtgc tttttttgga
      721 ataaaaaccg acatacggct tcggccaagc tttttccctt ttaccgaacc gagcttcgaa
      781 tttgacataa cttgtgcaat ttgttccgga aaaggctgct caacttgcaa aaacgccgga
      841 tggattgaaa ttggcggagc gggaatggtt aaccagaacg tatttattgc ttcgggttat
      901 cccagaaata aatatcaagg ttttgcctgg ggttttggcc tggaacgcct tgctatgatg
      961 aagtataaaa ttgacgatat ccgctggatt gaaagcggtg atttgagatt tataaagcaa
     1021 ttctagcatg aaatattcat ataattggct caaagaactt tccgggacga aattgtcccc
     1081 tgaaaaattg tcggatcgct tgatgatgcg gtcgtttgaa tttgaggaga tgaaaaaaga
     1141 gggaaaggaa actcagcttg agtttgctat tcttcccaat cgcgctcacg atatgttgtc
     1201 gcatatcggc atggcgcggg aaatttgcgc ggtggaagga cggaagttta aacacgagat
     1261 tgccgcagtc gctactctcc ctcgcaatga caaaattagg gttgaaatca aagacaaaaa
     1321 gctttgccgg agatatatcg gcgccgtttt ggaaaatata aaagttgcgc cttcgccgaa
     1381 atggatacag gaaagacttt tggtttcggg tgtgaagccg atcaataata ttgtcgacat
     1441 cacaaattat gtgatgcttg agactggcca atccctgcat gcctttgatg cgaaaaaaac
     1501 aaccggaaac ataatcgtcc gcaaagcgaa aaaaggagaa aaaataaaat tgctggatga
     1561 aaaagaatat gaactcggtg aaagcgatct tgtgatcgct gacagcgaaa aagcattggc
     1621 actggccgga gtaatgggcg gagcggaatc aagcgtggca agcaaaacaa cttccattat
     1681 tttggaatcg gccaatttca attcaaccaa tattcgaaag acaagaacgc ggcacaacat
     1741 catcacggaa tcttcgtacc gctttgaacg cgatattgat ccgaatcttg cagaaatcgg
     1801 tgcggcgcgg gccattgaac tgttgcaaaa atatggggga agcaatgcga agctggtcgc
     1861 atatactgat atatatccaa aaaaattgaa gccatggcag ataaaattaa acgccgatta
     1921 tgtaaatagt ctcttgggcg aaaaagttcc ggtttccaga attaaaaata ttctcgaaaa
     1981 tcttggcctt ggcgtaaaaa tttcgaaaaa tatattaaat tgcgaaattc caactgttcg
     2041 gattgatctc aaaacgcagg aggatctcat tgaggaaatc ggccggatct atggatatga
     2101 aaatatcctt gccaaagctc cg
//
LOCUS       DATKGS010000030         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_188277, whole genome shotgun sequence.
ACCESSION   DATKGS010000030 DATKGS010000000
VERSION     DATKGS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188277"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VMQ48_00695"
     CDS             <1..387
                     /locus_tag="VMQ48_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08591.1"
                     /translation="RGNVKSAVAAEKNIQKAEEDAALKESQDQEAAAAEAALAKAQAD
                     KAARAKMIVRQRAAELARINSAPVLAGATDKLSNGLRVCPLKNPHPSGRGGKPHIDED
                     CCADYDEYPNPRCEYSPSQMAALKKG"
     gene            403..1170
                     /locus_tag="VMQ48_00700"
     CDS             403..1170
                     /locus_tag="VMQ48_00700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUD08592.1"
                     /translation="MYISKNAKNIFLGLAVSASVFFLAGCGENQPPQQEVLRDAQQYD
                     IKSYQQRAPDTSPATHRLSVSAPQGDPGAAAAGQDPGVNQTDTKQPPAMQIDKNKTYS
                     AIIKTSEGDITIDLDAKKTPITVNNFVALARQNFYDGTVFHRVMKDFMIQGGDPNGDG
                     SGGPGYKFADESLGGKYTRGTVAMANSGPNTNGSQFFIIQKDYDLPSSYVIFGHVSSG
                     MDAVDKIAQAKVVDNGKGEKSKPVKPVAMDSVQIVEK"
     gene            1288..2232
                     /locus_tag="VMQ48_00705"
     CDS             1288..2232
                     /locus_tag="VMQ48_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUD08593.1"
                     /translation="MNPNCCKGCKKPAGRISLSSCKRFFGRAVEKAPFGVAAVKDGIV
                     LYSNLACAEILGYDARREVIGTNIIDHVAPEKRSEVVSFIKDIRENGLSVPRDGFFET
                     IVEEKSGSKIQLRIRLTREETRCCELVTLCYVTNVTEQKRKEEGYYNLAIRDHLTGLF
                     NRLYMEETLRREIARARRQGSGMGVVMLDLDHFKNFNDTYGHAGGDELLRAFAQLLNN
                     NVREEDVVCRYGGEEFLIIMPGASHEVVKSRAELIRQETAELKIYFQKNMLSSTTVSV
                     GIAFFPDHGGKEEKIIAAADQALYRAKAEGRDRVVVAE"
BASE COUNT          802 a          609 c          752 g          652 t
ORIGIN      
        1 agagggaatg tgaaaagcgc ggttgcggcg gagaaaaata tccagaaagc cgaggaggac
       61 gcagctttga aggaaagcca agatcaggag gcggcggctg cggaagctgc cttggcaaag
      121 gcgcaagcgg acaaagccgc cagggcgaag atgattgtga gacaacgcgc cgcggaactt
      181 gctcggatca atagcgcgcc ggttcttgcg ggggcaactg ataagctttc caatggattg
      241 cgggtttgtc ccttgaaaaa tccccatccg tcaggccggg gtggcaaacc tcatatcgat
      301 gaagattgct gtgccgacta tgatgaatat cccaatcctc gctgcgaata ttctccatcc
      361 cagatggcag ccctaaagaa aggataaaat taaaacatct tgatgtatat atcaaagaac
      421 gcaaaaaata tttttcttgg cctagctgtt tctgcaagcg tttttttcct tgcgggctgc
      481 ggcgaaaatc agccaccgca gcaggaagtg ctccgggatg cccagcagta tgatataaaa
      541 tcatatcaac agcgcgcgcc ggacacttcg ccggcaacgc accgtctctc cgtgagtgcg
      601 ccgcaggggg atccgggtgc ggcggcggcc ggccaggatc cgggagtaaa tcaaactgat
      661 accaagcagc cgcctgccat gcagatcgac aagaacaaaa cctattcggc aatcatcaaa
      721 acgagtgaag gggacatcac aattgatctt gacgcgaaaa aaactccgat cacggtgaac
      781 aatttcgtcg cgctcgcgag acaaaatttc tatgacggaa cggtttttca tcgcgtcatg
      841 aaagatttca tgattcaggg cggtgatccg aacggcgacg ggagcggtgg ccccggatac
      901 aagtttgccg atgaaagtct cgggggaaaa tatacgcggg gaaccgtcgc aatggccaat
      961 tccggaccca acacgaacgg cagccagttt ttcatcattc aaaaagacta tgatcttccg
     1021 agttcatatg tcatcttcgg ccacgtttcg agcggcatgg acgcggtcga caaaattgcg
     1081 caggcaaaag tcgtcgacaa tgggaagggc gaaaaatcaa agccggtgaa gccggtggcg
     1141 atggattcag tgcagatcgt ggagaaatga taggtgaatg cgggggcttg accagcctct
     1201 ttttttgtgc tagaatataa cattgcggca gtgcgtcgca aagcacctga aaaacagcct
     1261 aaaaggctat aaataaggga gaaagagatg aaccccaact gctgtaaagg atgtaaaaaa
     1321 cctgcaggaa gaatctctct ttcctcctgt aaaaggtttt ttggccgcgc ggttgaaaaa
     1381 gctcctttcg gagtagccgc tgtgaaggat ggaattgtcc tttattccaa cctggcttgt
     1441 gcagaaatac tgggatacga tgcccgccgt gaggtgatcg gaacaaatat tatcgatcat
     1501 gtagcgccgg aaaaacgtag cgaggttgtt tcatttatca aagacatccg tgaaaacggt
     1561 ttgtctgtcc ctcgcgatgg gttttttgag accattgtgg aagagaagag cggatccaag
     1621 attcaattga gaatccgttt gacccgggaa gaaacccggt gctgcgaact tgtcactctc
     1681 tgttatgtca caaatgtgac ggagcagaag agaaaggaag agggttatta taacttggca
     1741 atccgagatc atctgaccgg tcttttcaat cgtctgtata tggaagagac acttcgccgg
     1801 gaaatagcac gtgcccgtcg acagggatcc ggcatgggtg ttgtcatgtt ggatctcgat
     1861 cacttcaaga attttaacga cacgtatggt cacgccggag gagacgaact tcttcgggcg
     1921 ttcgcgcagc ttctcaacaa caatgtgcgc gaagaggacg tggtttgccg ctacggcggg
     1981 gaggaatttt tgatcattat gcccggggcg agccacgaag tagtgaaatc cagagcagag
     2041 ttgattcgcc aagaaacggc tgagctcaaa atatattttc aaaaaaatat gttgagttcg
     2101 accactgtct ccgtcggtat cgcgttcttt cctgatcacg gcggaaagga agaaaaaata
     2161 atcgccgccg ccgatcaagc cctctatcgt gctaaagcgg aaggccgcga tcgggtggtt
     2221 gtggcggaat aagttctttg agtatggatt aggggcggtt tgtgcaattt tgcaaaccgc
     2281 cctttcattt tggaaaaatt ctgtgtcacg ggaaaagata ttgccaaaaa cactttctcg
     2341 ccgtataatg caggagcatt tatgcacatt aaaatagact aataacattc ccgggtagct
     2401 caatggtaga gcagttggct gtaactggtt tgcagcttcc taaggaaact taggttgaaa
     2461 aattttggga taacggtgaa ggctaaatcg ctcaagaaat tgaggggtat gcgaacaccg
     2521 tgggaacttc gatacactat tgaagagcgc cgtagagact agataccaaa atgtctaagt
     2581 ccttttatta ggaaaagaca atgatatagt ccaccccttt tggaaacatt aggacaagtg
     2641 taaccaattg gttacaggtt cgaatcctgt cccgggagca ccgatattca ggcttatata
     2701 aagcctgatt tttgttaacc attgacacat tggttaacat agattaaaat tgggttatgg
     2761 ataaaaaata tttaacgaca tctgaattag ccaagctttt gggcatcagt cggat
//
LOCUS       DATKGS010000031         8826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_160684, whole genome shotgun sequence.
ACCESSION   DATKGS010000031 DATKGS010000000
VERSION     DATKGS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160684"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VMQ48_00710"
     CDS             complement(<1..285)
                     /locus_tag="VMQ48_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08594.1"
                     /translation="MESSKNIAIKDRITHAIFTGAAVMLVVFLISLALPKQWLVSGKI
                     VVFPSGQPASASQNLLAETGNTAEIIKNASFQKTNFPDQAANFAEARVPPS"
     gene            complement(347..1207)
                     /locus_tag="VMQ48_00715"
     CDS             complement(347..1207)
                     /locus_tag="VMQ48_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HUD08595.1"
                     /translation="MFINFFKKFKNILKTVALAALVFLAVYAILYFQTSWNTKPVFEK
                     AAPAEQPSFSGVIEVDENTLAAEAGITMADYNIWAGQNGLKGANAKLNADPDGDGLPN
                     YLEYIHGTDPNKADTDGDGFTDKQEITNGYDPAAPGDARPAVEVSIAKISVTAPMIWS
                     KNDNENDQLADLANGVAHFPQTASPGQDGNVIISGHSSNYIWAKGSYNYIFKNLNNLM
                     PGDVVDVKTVQQNGKIITYHYKISEKFTAMPDDARIFDNTDSPTLTLSTCWPLGTNLR
                     RLIVKGDLTQ"
     gene            complement(1330..1983)
                     /locus_tag="VMQ48_00720"
     CDS             complement(1330..1983)
                     /locus_tag="VMQ48_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08596.1"
                     /translation="MSKKNKVIKKALAIAVMVLFLGTLKAMASSSAASSSAASSAAAA
                     SGSGSNNSAAAASAAASGSGNHSVAPAAAAASSAASAAAAASGSHSPAVAAAGSSAAA
                     AASAGSGSSAAAGSAAASAGGSSAASAAAASAKTDDNDHNDNHNCKSDCDKDHNHNYH
                     YDKNHKRYYKDNDGKVVYVKTLPATGAGAAGMAGFAGIASLAAVGIKKYKLLSLLKR"
     gene            complement(2288..2887)
                     /locus_tag="VMQ48_00725"
     CDS             complement(2288..2887)
                     /locus_tag="VMQ48_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08597.1"
                     /translation="MKSSEMKVLEAESSLFVYNGVTQGVNKDGQVGVQLTLIGQAKAP
                     SQTVTTQQVPVTVTTQAAAPQRPVYSRDTRAAKAVSRTPPASPPVTKTEMQTVVTTTT
                     TFRGPEVLASTGGGGPSTGHSVAAAIPGALAGVGSAAVGGAFFQAGMKALRPAQTNVT
                     QTGGGATATGGAGGGGGSSSSSSAAAAAASAAAAAAAGP"
     gene            complement(2953..3354)
                     /locus_tag="VMQ48_00730"
     CDS             complement(2953..3354)
                     /locus_tag="VMQ48_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08598.1"
                     /translation="MKWLTIFVFIGLLFVGQASRVEASEGVKTNLQMANEDIETIDKI
                     GKISDPKVQARAIAEFMRKVQGFGDPVRVEAAKNGLNKYAFNMHDGKWAVIYESKKGL
                     NGASSGYIMAGENPPSDAEILPTKTIEGVKK"
     gene            complement(3506..3907)
                     /locus_tag="VMQ48_00735"
     CDS             complement(3506..3907)
                     /locus_tag="VMQ48_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08599.1"
                     /translation="MGRKVRSLIVRRKRFLPGPAVKSPPGIKKHGVGHSYALVVDMTG
                     TLLINPEITFSSAVPIRASSDDPENINNLAVFHGFAKLLLLPDPDVLAGDIVSGKAGL
                     AKKKKGEKNRKNILHGQSSFQRYFVQANSLF"
     gene            complement(4048..5409)
                     /locus_tag="VMQ48_00740"
     CDS             complement(4048..5409)
                     /locus_tag="VMQ48_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HUD08600.1"
                     /translation="MAKKYSVKFMVIFWAIAILLLTGWYVFLQVKNRNIENLKPFVKI
                     APVGSERRSEIATLMDIYSKIGNSGEEKNLLVLFQNDLELRPGGGFIGAFGMIKTKNG
                     KIADIQVFDTGNFDKNIPNTETPPTPLTDILGTEAWKMRDSNWSPDFKIDAEKAEYFY
                     KLGGGAESIDGVVAINTQVLNSVLAITGPVKIEGYPGEYRDENAILQLEYQVEKGYAE
                     QGIEKAGRKNIMGELADVLAQKAQTLSLPQQLALAQKIEDHLKQKDIQLFFNDENLQK
                     EIEDIGWGGRVRDFDGDYLMAIDANMLSLKSDYCIKRKMDYSVDLSTDTPQAVLNITY
                     EHTCRAKDWMTTDYRDWLRVYVPKDSYLEEANGQSGDVQYAEELGKKVFGMRVDVPVG
                     ETKTITLRYKLPQSLKNNNYQLLVQKQSGSGEVPFQISVKKTDGSDVKAQETLTGDKE
                     FGF"
     gene            6139..6501
                     /locus_tag="VMQ48_00745"
     CDS             6139..6501
                     /locus_tag="VMQ48_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08601.1"
                     /translation="MRPSACGKIFPRIPYMVSAARHRNQPGLKDGQGGTKAGCSGRKS
                     LGVGVLEGFSVGRERNHTNGKSCTLARRLAPTRLRKKVQSPIFKSGAGKGDGGKVKCP
                     NTERVLPRLTHASRSPSG"
     gene            complement(7299..8627)
                     /locus_tag="VMQ48_00750"
     CDS             complement(7299..8627)
                     /locus_tag="VMQ48_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUD08602.1"
                     /translation="MNQFSKRPTIGQKKYFYSLLLPNVRSFHEWGIVFRLAFRNLKTN
                     KMRTVLTVLGIVIGITAVIIVMAGGAGLKNYVMGQVDVFGSDYIQIEPKVPGVSDTSS
                     QNISGRSTGLAITTLKIADGEEIAKLPNVAAWNAGNIDQEIAVYKNINKRSLIFGYMP
                     GALAIDRQLVIEEGNFFTEGENASAEQVAVIGSGVRDSLFGEEDPIGKSVKIKSQSYR
                     VIGLAKKRGAGGFFNLDDAVYVPVNTLNKKLLGIDYVQFITVQLKDMSLVTESEADIR
                     DTLRRRHDIIRADKEDFAITSSVEAKDMINNVFGTINYLLLALTSISLLVGGVGIMNV
                     MYVAVTERTREIGLRKALGAKNSSILRQFLFEAVIVTILGGAVGVIFGIAATYFLNSL
                     LVRLGFLLQFSVPISTIVVAVGFSAAVGVIFGIYPAWKASQLSPIEALRK"
BASE COUNT         2218 a         2195 c         2063 g         2350 t
ORIGIN      
        1 ggaaggaggg actctcgcct ccgcaaagtt cgcagcctgg tcgggaaaat tcgttttttg
       61 aaaagacgcg tttttgatga tttctgccgt gtttcccgtt tccgccaaaa gattctgcga
      121 ggcgctcgcc ggctggccgg aagggaaaac cacaattttt ccgctcacaa gccactgctt
      181 gggaagagcc aatgagatca aaaacacaac aagcatcacc gcggctcccg tgaagattgc
      241 gtgagtgatc cgatctttga tagcgatgtt ttttgaagat tccatggttt tgaattataa
      301 tttgttagtt ggtcattatt atcgataatg aattttttgt caaatatcac tgagtcaaat
      361 cccccttcac aatcagtctg cgcaaattcg tccccagcgg ccagcaagtc gaaagagtaa
      421 gtgttggact gtctgtattg tcaaatatcc tcgcgtcatc cggcatggcc gtgaattttt
      481 cacttatttt ataatgatag gtgatgattt tcccattttg ttggactgtt ttcacgtcca
      541 ccacatcgcc cggcatcaaa ttgttgagat tcttgaaaat ataattgtag ctgcctttcg
      601 cccagatata gtttgaacta tgtccggaaa tgatcacatt gccatcctgg ccgggagaag
      661 ccgtttgggg aaaatgagcc acaccattcg cgaggtctgc cagctgatca ttttcattgt
      721 cattttttga ccaaatcatc ggcgcggtga cgcttatttt tgcaatcgaa acttcaaccg
      781 caggacgcgc gtcgcccggt gcggccggat catatccgtt ggttatttcc tgtttgtcag
      841 tgaatccatc gccgtccgta tccgctttgt tcgggtcagt tccgtggata tattccagat
      901 agttcggaag tccgtcgcca tccggatcgg cattcaattt ggcgttggcg cccttcagac
      961 cgttttggcc ggcccaaata ttataatccg ccatcgtgat gccggcttcg gccgccaaag
     1021 tgttctcatc aacttcgatc acgccggaaa aagacggctg ttcggctggc gctgcttttt
     1081 caaacaccgg ttttgtgttc caactggttt gaaaatataa aatagcgtat acggccaaaa
     1141 agaccaaggc agccaaagcc acagttttca gaatattctt gaattttttg aaaaagttta
     1201 taaacatagg aatatttttg catagagaca tattctcctg ctcgtaaaga tgcaaccaat
     1261 cacgtcttta cgagcaggag caaggttgaa ccttgctcct gttgtttctt tttgattttt
     1321 caggctacgt tatctcttca gaagagaaag taatttgtat ttcttgattc caaccgccgc
     1381 gagagatgca atccctgcga atccggccat tccggccgcg cctgcacctg ttgccggcaa
     1441 ggttttcaca tagacgactt ttccgtcatt gtctttgtaa tagcgtttgt ggtttttgtc
     1501 atagtgatag ttgtggttgt gatctttgtc acaatcgctt ttgcaattat ggttgtcatt
     1561 gtggtcattg tcatcagtct ttgcactggc agcagccgca gaagcggcag aagagccacc
     1621 cgcagaagca gcggcagatc cagcggcggc tgaagaaccg gaaccggcag acgcagcggc
     1681 ggcagcagaa gagccagcag cagcaacggc aggagaatgt gatccggaag cggcagcggc
     1741 ggctgaagca gcagagctgg cagcggcggc agcaggagca acagagtgat tgcctgatcc
     1801 ggaagcggca gcgctggcag cggcagcaga attgttcgag cctgatccgg aagcagccgc
     1861 ggcagaagat gcagcggagc tcgaagcagc cgaactcgaa gccatagcct tcaatgttcc
     1921 tagaaacaga accatgaccg cgatagccag tgctttcttg ataaccttgt tctttttcga
     1981 catagtatta taaaattact tattaataaa cctaaactag gttgattgag tatattagca
     2041 ttagaattgc tttcttgtca agcgggattt ccccacaaga agacgaaacg gaataattgc
     2101 taaaataagg cttatatata gtcttttatg gcctcctata ttccttgttt ttcaattttt
     2161 atcgaaactt atattttttg aaaaagatat tgaaaattcc gtagttcttg tagacaaaaa
     2221 gaaaaacccc taatctctgc gagacgaggg gtcttgttgt gcccgatttc gggtctttca
     2281 ttaggcatta agggccggct gctgctgcgg ctgcggcaga tgctgcggcg gcggctgcgg
     2341 aactcgaact ggaacttcct ccacctccgc ctgctccgcc agtggccgta gctccgccgc
     2401 cagtttgagt aacgttcgtt tgggcgggcc ggagggcttt catgcccgct tggaaaaaag
     2461 ctccgccgac ggccgcggat ccgacacctg ccaatgcccc cggaatagcg gcagcgacag
     2521 aatggccggt cgacggacca cctccacccg tcgaggccag aacttccggc ccacggaacg
     2581 tggttgtggt cgttacgacc gtctgcattt cggtctttgt gaccggagga ctggcaggcg
     2641 gcgtacgaga aacggccttg gccgctctag tgtcccgact gtagaccggc ctttggggcg
     2701 ccgcagcctg cgtggtcacc gtcacgggca cttgctgcgt ggtcacagtc tgggaagggg
     2761 ctttggcctg gccaatcaga gtcaactgaa cgccaacttg gccatccttg ttgacccctt
     2821 gggtcacgcc gttgtacaca aataggctgg attccgcctc taagactttc atctccgagg
     2881 atttcatgac agtaccacag gctgaaatcg cgaaacaaac catagccaat actgcaaaat
     2941 ttctcacggt tctcattttt tcactccttc aatcgttttt gtcggtaaaa tttcagcatc
     3001 cgagggcgga ttttcacccg ccatgatata gccacttgat gcgccattca accctttttt
     3061 gctttcatag ataacggccc acttgccgtc atgcatattg aaagcatact tattgaggcc
     3121 gtttttcgcc gcctcaactc tgactggatc accaaagccc tggacttttc gcataaactc
     3181 cgcgattgcc cgggcttgga ccttcggatc ggatatcttc ccgatcttat cgatcgtttc
     3241 gatatcctca ttggccattt gaaggttggt tttcacaccc tcactagcct ccacccggga
     3301 ggcctggcca acgaataaca ggccgataaa aacaaatatt gtcagccatt tcataggccg
     3361 tcctcctcta tttatttatt aaaatactgc ctctttgagg cttgaaataa gctaaattcc
     3421 gctttagatc atttcaagtc tcacaaatat attttccacc ccaacagacc agtatatacc
     3481 ctattgacat tcttgtcaag gggtgttaga atagactgtt ggcttgaaca aaatatctct
     3541 ggaaggagga ctggccatgc agtatgttct ttcgattttt ctcgcctttt ttcttctttg
     3601 ccagtcctgc tttgccgctg actatgtcac cggcaagcac atctggatct ggcaagagca
     3661 gcagcttggc gaagccgtgg aagacggcga ggttatttat attttccgga tcatcactgg
     3721 acgcgcggat aggtaccgca ctcgagaagg tgatttccgg atttatcaaa agagtccccg
     3781 tcatgtcaac cacgagggca tagctatgcc ctactccatg ttttttgatg ccaggcgggc
     3841 tcttcacggc tggtcctggg aggaaccttt tccttctgac aatgagagac cgtaccttgc
     3901 gtcccatgga tgcgtaagca cggaaattcc ccgggagctg tttgaatggg cccctatggg
     3961 aactcctgtc catgtccgcg gcgagcggat cggctacaat tgaaccccgg cgggttggat
     4021 aaattctcca acccgctttt ctttttctca aaacccaaat tccttgtccc ccgtcaaagt
     4081 ctcctgcgct ttcacgtcac ttccatccgt ctttttgacc gaaatttgaa acggaacttc
     4141 gcccgatccg gattgttttt ggacgagaag ttgataatta ttgtttttta gagattgcgg
     4201 aagcttatat cttagcgtta tcgttttggt ttccccgacc ggaacatcca ctcgcattcc
     4261 aaagactttt ttgccaagtt cttcggcata ttggacatca ccgctttggc cattcgcttc
     4321 ttcgagatat gaatcttttg gaacgtaaac tcgcagccag tcgcgatagt ccgtcgtcat
     4381 ccaatctttc gcgcggcaag tgtgttcata agttatattt agcacagctt gaggcgtatc
     4441 cgtagaaagg tcaacgctat aatccatctt cctcttgatg caataatcgc ttttgagcga
     4501 cagcatattc gcatcaatcg ccatcagata gtcgccgtca aaatctctga ctcgcccgcc
     4561 ccagccaatg tcttcaatct ctttttgcaa gttttcatca ttgaaaaaaa gctggatatc
     4621 tttttgcttg agatgatcct ctattttttg ggcgagcgca agctgctgcg ggagactcaa
     4681 cgtctgcgct ttttgggcca aaacgtcggc caattcgccc atgatatttt tgcgccctgc
     4741 tttttcgatc ccctgttcgg catatccttt ctccacttga tattcaagct gaagaatcgc
     4801 gttttcatcg cgatattctc ccggatagcc ctcgattttc actggacccg tgattgccag
     4861 aacagaattc aaaacctgcg tgttgatcgc aaccactcca tcgatgctct ctgcgccacc
     4921 acccagttta taaaagtatt ccgctttctc ggcgtcgatt ttgaagtccg gcgaccaatt
     4981 ggaatcgcgc attttccaag cttccgtccc gaggatatcc gtgagaggcg tcggcggtgt
     5041 ttccgtgttt ggtatatttt tgtcaaaatt ccccgtgtcg aagacttgga tatcggcgat
     5101 ttttccgttt ttcgtcttga tcatgccgaa cgcgccgatg aatccgccac ccggacgaag
     5161 ctcaagatcg ttttgaaaaa gaacaagaag atttttttcc tcgccggaat tgccaatttt
     5221 gctatatata tccattaaag tcgcaatctc actgcgcctt tcacttccca ccggtgcgat
     5281 tttgacaaaa ggcttaagat tctcgatatt tcgatttttc acctgcaaaa agacatacca
     5341 tccggtgaga agcaggattg caattgccca aaaaatgacc atgaatttta cactgtattt
     5401 cttcgccata taacaactca atttagcaaa tacgcggaag ttgtcaatga tttaaaatcc
     5461 tcctcccctg ccaaggggag gtgcccgaag ggcggagggg tttgttttcg cacaaagaaa
     5521 accacccctc ttatcccctc cttggcagga ggggaggatt tgttctatat ttttacttta
     5581 tacaagccat tatctcgcct catcggcggg caaatttgca ttttttcgga ctttttcttg
     5641 acacccactt gacagatttt taccgatctg ctaggatatg tgcacatttg aaaaagttct
     5701 gtaacaatca aacactcaca caactcagga ggtgctttat gcacaaacag actaccggtc
     5761 ggcaaatgtt gatcatcgat tccagcaccc tgaatctgaa gtcagcgttc cccttcggca
     5821 ccgccccggc ctacagcgac gcctaaaggc accggcccgg ggagccggcg ggatgaaccc
     5881 gctggctcag ccaaagagag agggaaaagt agtatgcggg ggattcccgc gaaaaaatct
     5941 gcccaagtgg cctaactcaa aacggaggtc caccactgtg gttctgaaag tctacgatta
     6001 gtaaagtagt gggcaacggc acaccggtga ggttgagact acacctcccg gatccggatt
     6061 cgattttttc aaagggcaac tccctgccgg cgcccccaac cagtggggca ggggcaggat
     6121 agcacgtagg tgggaaagat gcggccatcg gcctgcggga agatttttcc gcggatcccg
     6181 tatatggtct cggcggcccg ccatcgaaac cagcccggcc tgaaagatgg tcagggcggg
     6241 acgaaggctg gctgcagcgg ccgcaaatcg ctgggggtcg gcgtgctgga ggggttttcc
     6301 gtcggaaggg aaagaaacca tacaaacgga aagtcttgta ctttggcccg acgtctggcg
     6361 ccaacccgcc tgcggaaaaa agtccagtcc cccattttta agagcggggc tggcaaagga
     6421 gacgggggca aggtcaaatg ccccaacacg gaacgggttc taccccggct gacgcacgct
     6481 tcgcgatcgc cgtcgggatg accccggatg tagtatctcc gtattccgaa aggaatcggg
     6541 ggaaaggtct ggaagccaac cggtttccag acctatttta tttttcaaaa gcttgacacc
     6601 cttttcgaat ttgcataaaa tacaattaga ttagtccaat agttctttca taccaccgtc
     6661 ttaaccccga tgtgtggtgc agaaattaga agggaatcta atatacttta ttagaaccca
     6721 ccatcctccg gagaattttt ttagacccaa ccctcaactt ctccggaggg ttttttattt
     6781 tccgttgaca gaaaatttga aaggagtaaa ttgaaaataa gcgaaatttt cgcaaataaa
     6841 aaatgagtct tgcgccacga cgagaccggc tctgcgaaaa cgacgccgct ccccggagaa
     6901 gatattatac tgatcgctga tcagataaga atcatcttcg ggggcttata ttttctcaaa
     6961 gaatgaaagc aaaaaggcca gtttgcgtat gtataattag gaaaggaatc caaaaacctt
     7021 aacaattgaa gcaaaattat gaccagccat taacatggcc acctctctgg gtaggaagaa
     7081 aagtaaattt gtgttgtgtt gattatcttt gaggaggaag gggtagttag catggtgcga
     7141 aaatggaaga gtttccaaac agacaacgat cgagaatttc ttagaaatct gaagtcaacg
     7201 agcgagaggt ggttttttct atattataaa gcaaaaaaga taaaatgttt cacaaaatat
     7261 tgtttcacaa gtcagcctga agggctgttt ttttgtgact actttcgcaa tgcctcaatc
     7321 ggtgagagtt gcgacgcctt ccaggcggga tagatgccaa aaatcacacc cactgcggct
     7381 gaaaatccga ccgcgacgac gatggttgaa attggcacgg aaaattgaag aaggaatcca
     7441 agacgaacaa gaagactgtt gagaaaatag gtggccgcaa tgccaaaaat gactccgacc
     7501 gctccaccga gaatcgtgac gatcaccgct tcgaacaaaa attggcgcaa gatgctcgag
     7561 ttcttggctc ccaaagcttt gcgcagtccg atttcgcgcg tccgttcggt gacggccaca
     7621 tacatcacat tcatgatacc gactccacca acgagaagag aaatagaagt gagggcgaga
     7681 agaagatagt tgatcgttcc aaaaacatta ttgatcatgt ccttggcttc aactgatgag
     7741 gtgatggcaa aatcttcttt gtcggcgcga atgatgtcgt gccggcgtcg cagagtatcg
     7801 cgaatatccg cttccgactc tgtgaccagc gacatatcct tgagctgcac cgtgatgaat
     7861 tgaacataat cgatcccgag caattttttg ttgagcgtat tcacgggaac ataaacggcg
     7921 tcgtcaagat tgaaaaatcc gcccgctcct ctttttttgg ccaaaccgat cacgcgataa
     7981 ctttggcttt tgattttgac gcttttcccg atcgggtctt cctcgccgaa caagctgtcg
     8041 cgcacgcctg atccgatcac cgccacctgc tcggccgatg cgttctcacc ttcagtgaaa
     8101 aaattccctt cttcgatcac aagctggcgg tcaattgcca atgctcctgg catatatccg
     8161 aagatgaggc ttcttttgtt gatgtttttg taaaccgcaa tttcctggtc tatgtttccg
     8221 gcgttccaag ccgcaacatt cggcagcttc gcgatttcct cgccatccgc gattttgagc
     8281 gtcgtgatcg ccaggcccgt tgaccggcca gaaatatttt gcgaggatgt atccgaaact
     8341 cccgggactt tgggttcgat ctggatataa tcactcccaa aaacatcaac ctgccccatt
     8401 acataattct tgagacccgc cccacccgcc atcacaatga tcactgcggt gatcccgatc
     8461 acgattccaa gaaccgtgag gactgtccgc atcttgtttg ttttgaggtt ccgaaaagca
     8521 agacgaaaaa caattcccca ttcatggaaa gaacgaacgt ttggaagaag aagtgaataa
     8581 aaatattttt tttgtccgat tgtcggacgt tttgaaaatt gattcattga aacattggaa
     8641 attgtttgaa aatttgtaaa ttgaaaattg aaaattttgc gctattcata gcgcaatgcc
     8701 tccactaaat tgagttttga tgcttttctc gccggataga ggccaaagaa aattcccacc
     8761 aaaaaagaaa tgccgacagc cagagcaatt gaagaaaatg tcaccacaaa aggccaattg
     8821 tatccc
//
LOCUS       DATKGS010000032         6254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_30430, whole genome shotgun sequence.
ACCESSION   DATKGS010000032 DATKGS010000000
VERSION     DATKGS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30430"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VMQ48_00755"
     CDS             complement(<1..998)
                     /locus_tag="VMQ48_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUD08603.1"
                     /translation="MPNLLRKIKNTSETNHLMGFLFDKIAGLSIYRKFIDYKIRRYAR
                     RIKKNKAFNIIIETTSICNAGCVMCPHTAMKRKKELMRDETFDLIIKKLKREEIGPLA
                     VILNGFGDPLTDQKIFDRVRTIKKEFPGTIVKFYSNLGLANDKVIENILNSGLDEINV
                     SFNGYDREGYEKTMGIDFQKTLDNLKNLIAEKNKAQSLLKIRISMALVSHNTGDEKKF
                     IKFWRDKVDSVSVNKIHTYNNSVEDVSGKNKINFRKPAYPCKYLWNTIVFGTGGDIYL
                     CCLDYEGRYNFGNISENEILDVFYGEGFNNIREKHLKNDVKSIDMCSACYTPYKNGA"
     gene            complement(1003..1596)
                     /locus_tag="VMQ48_00760"
     CDS             complement(1003..1596)
                     /locus_tag="VMQ48_00760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HUD08604.1"
                     /translation="MHKLFQILGKIQTFLLKKKFHHFGDGSIIKPFLNSANEKYISIG
                     DNVNIGSNCRITVSTEFGGHKVKSDNKIRIKIGDNVDIGNNTFISANNSVEIGNHVII
                     SSYVFITDHDHGFQDFSRNLHEQPLTEGSFVKIGNNVFLGTKCSILKNVTIGERSVVA
                     ANAVVTKDVPACSIVAGNPARLIKKFDFEQNKWTKPQ"
     gene            complement(1589..2278)
                     /locus_tag="VMQ48_00765"
     CDS             complement(1589..2278)
                     /locus_tag="VMQ48_00765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUD08605.1"
                     /translation="MEKMINKLRKSRLPGKIIHTLDYCLQWELKDCESVLDLGCGPSS
                     PLQYCKNIKYSIGVEAFQPYLEESKRKNIHTEYLKQRIEEVDFSRDSFDAVIMIEVLE
                     HLTKETGLEILEKAEKWAKNKIIISTPNGYFPMGAVDSNAFQRHLSGWNIDALEKLGF
                     KCYGVSGLKSLYLKENIVHSLNNEKDNTFSNIRFRPKKFFYVINGLLQVFSYYFPKKA
                     FGLFAIKNKNA"
     gene            complement(2282..3298)
                     /locus_tag="VMQ48_00770"
     CDS             complement(2282..3298)
                     /locus_tag="VMQ48_00770"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD08606.1"
                     /translation="MMNDLKFSILIPTYNGAEVIGDTLRSLLSQSFQNFEIIIQDDAS
                     KDSTIEFIKSFKDPRIKIYQNAKNIGYSENLESLSKKAAGDIIYLMGQDDILGENALL
                     ETYYAFKISDDIGAVARPYFWFDEKITKPVRATGQLNPEKNEIVTIKDDFARIILTID
                     VAGQLSGLALRRKYLDTPFHKDIFPCHVHPFAAILKKHPIVFLKDYNLAVRIRSSQCR
                     SVSSIYDKSPVESWVDFWNNVFPEKEFEPLRKYAIKNYVANNYVGLVQIRNYARYKYL
                     LREIRMLLKYRWQNIYSPSFWFFSLGCMIMPPFLLIPLVDWYKSKINSKKLKHIKFQH
                     SMLG"
     gene            complement(3295..4128)
                     /locus_tag="VMQ48_00775"
     CDS             complement(3295..4128)
                     /locus_tag="VMQ48_00775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08607.1"
                     /translation="MLVSVIVSTKNEEKNIEKCLKSVVDQSFAAENIEIIIVDNNSTD
                     KTKEIAEKYTEKVFSLGPERSAQKNFGIRKSKGEYFLHLDADMTLSENVVQECVEKVS
                     KDKNIIALYIPEIVTGRSFFSKVRRFERGFYDGTTIDAVRFIRKDKFLAAGGFDEKMY
                     ACEDWDLDKRLEKFGKFDIIKSPLYHNEEEFNLKEYLAKKGYYSKNLDAYISKWGKND
                     PDIKKQFGFSYRFFGVFIENGKWKKLLQHPIMLVGMYFLRLMVGLKYLLRNKKSRTLK
                     R"
     gene            4200..5936
                     /locus_tag="VMQ48_00780"
     CDS             4200..5936
                     /locus_tag="VMQ48_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUD08608.1"
                     /translation="MSLDNRQKQYIKKKLRKIPLAEIAADIEVSEDDILEYFKKKWPR
                     EKYERFLKGKFEIEVASGGAGASFSFLNFFNENRLVLLFLAALVLVVYANSFGNGFVS
                     DDVAGFLRNENLGKFSSIFGGPMHFSLSAFFQFIAFHIGGLHPFTFRIINILSHIGCI
                     VLIYLLLSLSMNKRVATIAASIFAVHPILTESVSWISGGLYSLYAFFFLLSFLFYLFS
                     KSNKKYFKYAVVFFVLSVLSSEKALALFLVFALYEFSFGNLKYSWRKVMPFFSISFFL
                     LLFYVFKIGSRVSSVESQSYQSSGGMYNPFVQLPVAIGSYLKLIFWPARLTLYQTEMS
                     FSQGQYLILLFIFLVYIGAIFWSWRKNKNVFFWLVFFVITLLPTLTPLKISWIVAERY
                     VYLGTLGIIVVVAMLFDKILKLNENAKMIGYFALLVIVAALSVRTIIRNKDWKSEDTL
                     WIATAQVAPSGQQIHNNLGDVYARQGDMQKAVEEFKKAIEINPNYADAYHNLANTYQQ
                     MGQLDLAIENYQKALSINPQLWQSYQNLAAIYFNQGQYDLAEENIKKALEINPGDQNL
                     ESALKYIQENRK"
BASE COUNT         1623 a         1304 c         1218 g         2109 t
ORIGIN      
        1 gctccatttt tgtagggagt atagcaggct gaacacatgt cgatgctttt cacgtcattt
       61 ttcaggtgtt tttctcttat gttattgaat ccttcgccgt aaaaaacatc caaaatttca
      121 ttttcggaaa tattgccgaa attgtatctt ccttcatagt ccaggcagca caaatatata
      181 tcgcctcccg ttccgaaaac aatcgtattc catagatatt tgcacgggta agcaggcttt
      241 ctgaaattta ttttgttttt tccggaaacg tcttcgacgg aattgttgta ggtatgaatc
      301 ttgttcacgc tcaccgaatc aactttgtcc cgccaaaatt ttatgaattt tttttcatct
      361 cccgtattat gggataccag cgccatggaa attctgattt tcaacaagct ttgcgctttg
      421 tttttttccg ctatgaggtt tttcaggtta tccaaagttt tctgaaaatc tattcccatg
      481 gttttctcat aaccttctct gtcatatccg ttgaagctca cgtttatctc atccagccca
      541 gaattgagaa tattttctat gactttatca ttggcaagac ccaaatttga ataaaatttc
      601 acgatagttc cgggaaattc ttttttgatt gttctcacgc gatcaaatat tttttggtcg
      661 gtcaatggat cgccaaatcc attgagaata acggccagcg gacctatttc ttctcttttt
      721 agttttttga ttatcaagtc gaatgtttcg tcccgcatca attccttttt tcttttcatc
      781 gcagtatgtg ggcacatcac gcatccggcg ttgcaaatac tggttgtttc aataattata
      841 ttaaatgctt tgtttttttt gattcgtctt gcgtatcttc tgattttata gtctataaat
      901 tttctataaa tagatagtcc ggcaatctta tcaaacaaga aacccatcaa atgattggtt
      961 tccgaagtat tttttatttt tctcaacagg ttcggcataa gattattgcg gttttgtcca
     1021 tttgttctgt tcaaaatcaa attttttgat cagcctcgcc ggatttcctg ccacaatgga
     1081 acaggcggga acatcttttg tgacaactgc gttggccgcg acaacggacc tttcgcctat
     1141 ggtcacgttt ttcagtattg agcatttggt gccaagaaaa acattatttc caatttttac
     1201 aaaacttcct tcagtcaacg gttgctcgtg gagattccgg ctgaaatcct gaaaaccgtg
     1261 atcatgatcc gtgatgaaaa cataagagga tataatcaca tgattgccta tttccaccga
     1321 gttgttggcg ctgataaaag tattgttgcc gatatccaca ttgtcgccga tttttattct
     1381 gattttattg tcgcttttta ccttgtgtcc tccaaattcc gtgctgacag ttatgcggca
     1441 attggagccg atattaacat tgtcgccaat actgatatat ttttcattgg cgctgtttaa
     1501 aaacggcttg atgatcgaac catcgccgaa atgatgaaac ttttttttga gcaaaaaagt
     1561 ctggattttg ccgagaattt gaaataactt atgcattctt gttttttatc gcgaataagc
     1621 caaaagcttt ctttggaaaa taatacgaaa agacttgcag caggccattg atgacataga
     1681 aaaatttctt gggtctaaat cggatgttgg aaaaagtatt atccttctca ttattcaaac
     1741 tgtgcacgat attctctttc aaatacaaag atttcaatcc gcttacacca tagcatttga
     1801 aaccgagttt ttccaacgca tcgatgttcc agccggacaa atgcctctga aatgcgttgc
     1861 tgtccaccgc gcccatcgga aaatagccgt tgggagtcga aataattatt ttatttttag
     1921 cccatttttc cgctttttcc aaaatttcga gcccggtttc ttttgtcaga tgttccagca
     1981 cttcaatcat aatcacggcg tcaaaactgt cgcgggagaa atcaacttct tcgattcttt
     2041 gcttgaggta ttcggtgtgg atattttttc tttttgactc ttccagatat ggctggaaag
     2101 cttccacccc gatgctatat tttatatttt tgcagtactg gagaggcgaa gatgggccgc
     2161 agccaagatc caggacgctt tcgcaatctt tgagctccca ttgcaaacaa taatcaagag
     2221 tatgaataat ttttcccggc aatcttgatt tacgcaattt gtttatcatt ttctccataa
     2281 attatcctaa cattgaatgt tggaatttta tatgctttaa ctttttggaa ttgattttgc
     2341 ttttatacca atcgacaaga gggatgagga gaaacggtgg catgatcatg catcccaaac
     2401 tgaaaaacca aaaagagggg ctataaatat tttgccagcg gtatttcaaa agcatcctta
     2461 tttctcgcag caaatatttg tatcttgcgt aatttcggat ctggacgagg ccgacataat
     2521 tgttagcaac ataatttttg atcgcgtact ttcgcagggg ttcaaattct ttttccggaa
     2581 aaacgttgtt ccaaaaatcc acccagctct caacaggcga cttgtcatat atcgatgaga
     2641 cgctccggca ctggctcgac cggatgcgga cagcgaggtt gtaatctttg agaaaaacga
     2701 ttgggtgctt tttgagaata gcagcgaaag gatggacatg acaaggaaaa atatccttgt
     2761 gaaaaggtgt atccaaatat tttcttcgca aagccaatcc cgacagctgg ccggcgacat
     2821 caatggtcaa aattattctc gcaaagtcat ctttgatggt gactatctca tttttttccg
     2881 gattgagttg tccggttgcc cggacaggtt tggtaatttt ttcgtcgaac cagaaataag
     2941 gtcttgcgac cgcgccgata tcatccgaaa ttttgaaggc gtaataagtt tcgagcaagg
     3001 cgttttcgcc caaaatatcg tcctgaccca tcagataaat aatgtctccg gcggctttct
     3061 tgctcaagct ttcaagattt tctgaatagc cgatattttt tgcattttga tatatcttga
     3121 tccgcgggtc tttaaaagac tttatgaatt cgatggtact gtctttggat gcgtcgtcct
     3181 ggatgatgat ttcaaaattt tgaaaactct gcgaaagaag gctgcgcaaa gtatcgccga
     3241 tcacttcggc gccgttgtag gttgggatca aaatgctgaa tttgagatca ttcatcatct
     3301 tttcaaggtt cgacttttct tattacgcaa caaatatttt agaccgacca taagcctcaa
     3361 gaaatacatt cccactaaca tgattggatg ctgcaaaagt tttttccact ttccattttc
     3421 gataaatacg ccgaaaaatc gataagaaaa tccgaattgc tttttgatgt ccggatcatt
     3481 cttcccccat tttgaaatat acgcatcgag atttttggaa taatatcctt ttttggcgag
     3541 atattccttg aggttgaatt cttcttcatt gtgatatagc gggcttttga tgatatcaaa
     3601 tttcccaaat ttttcgaggc gcttgtcgag atcccaatct tcgcaggcgt acattttttc
     3661 atcaaatccg cctgctgcca aaaatttgtc tttccggatg aagcgtaccg cgtcaatcgt
     3721 cgttccgtca taaaatcccc tttcgaacct cctgactttt gagaaaaaac ttctccccgt
     3781 cacaatttcg gggatataga gagcgatgat atttttatct ttcgaaactt tttccacgca
     3841 ttcctgcaca acattttcgc tcaaagtcat atctgcgtcg aggtggagaa aatattcccc
     3901 tttcgatttc cgaatgccga agtttttttg agcggacctt tccggcccca ggctaaagac
     3961 tttctccgtg tatttctcgg ctatttcttt ggttttgtcg gttgaattgt tgtcaacaat
     4021 gatgatttct atattttcgg ccgcaaaaga ttgatcaacg actgatttca ggcatttctc
     4081 aatgtttttt tcctcatttt tcgtgcttac tatgacggaa accagcataa accatgttta
     4141 agattatcat tattcatagt ataatgtatt tgtcatagaa tccaaattta ttgaaactta
     4201 tgtctctcga taaccggcaa aaacaataca taaagaaaaa gctcagaaaa attcctttgg
     4261 cggaaatagc ggcggacatc gaggtttcgg aagatgatat tttggaatat ttcaagaaga
     4321 aatggccgcg ggaaaaatac gaaagatttc tcaaaggaaa atttgagatc gaagtagctt
     4381 ctggcggagc cggagcgtcc ttcagcttcc tcaatttttt taatgaaaac cggctcgtcc
     4441 tgctttttct cgccgctttg gttcttgttg tttatgccaa ctcttttgga aatggatttg
     4501 tttctgatga tgtcgccggt tttttgagga atgaaaatct gggaaaattt tccagcattt
     4561 ttggcggccc catgcatttt tcactctcgg ctttttttca gttcattgct tttcacatcg
     4621 gcggactcca tccatttacg tttagaataa tcaatattct ttcccatatt ggctgtattg
     4681 ttttgatata tcttttgctg tctttgtcga tgaacaagcg cgttgcgacc atcgcggctt
     4741 cgatttttgc cgttcacccg attctcaccg agtcagtctc ctggatttcc ggcggcctct
     4801 attcactgta tgctttcttt ttccttcttt cgtttctttt ttatcttttt tccaaaagca
     4861 acaaaaaata tttcaaatat gctgtcgttt tttttgttct ttctgtcctt tcttcggaaa
     4921 aagcgctggc tttatttttg gtgttcgccc tttatgaatt ttcgtttggc aatctgaaat
     4981 atagctggag gaaagtaatg cctttctttt caataagttt tttccttttg ctgttttatg
     5041 tgtttaagat cggtagtcgg gtatcctcag ttgagtcgca atcttatcag tcctccggcg
     5101 gaatgtataa cccgtttgtc cagcttcccg tggccatcgg atcatatctg aagctcattt
     5161 tttggccggc gcggctcaca ctctatcaaa cggaaatgtc attttcccaa ggacaatatc
     5221 tgatcctgct tttcattttc ttggtttata ttggagcgat cttctggagc tggcgcaaga
     5281 ataaaaatgt ttttttctgg ctggtctttt ttgtcatcac gcttcttccc accctcacgc
     5341 ctctcaagat ttcctggatc gtggccgaac ggtatgtcta tctcgggacg ctcgggatca
     5401 ttgtggttgt ggccatgctt tttgacaaaa ttctcaagct caatgaaaac gccaaaatga
     5461 ttggctattt tgcgctgctt gtcattgttg cagctctctc cgtccggacg atcatccgca
     5521 acaaagattg gaaaagcgag gacacgcttt ggattgccac ggcgcaagtc gcgccttccg
     5581 gtcagcaaat ccacaacaac ttgggagacg tctatgcccg gcagggggat atgcaaaagg
     5641 ccgtcgaaga attcaaaaaa gcgatcgaga tcaatccgaa ctatgcggac gcctatcaca
     5701 atttggccaa tacctatcag cagatgggcc agctcgacct ggcgatcgaa aactatcaaa
     5761 aagctttgag tatcaatccg cagctttggc agtcatacca aaacctggcg gcgatatatt
     5821 tcaatcaggg ccagtatgat cttgcggaag agaacataaa aaaagccctc gaaataaatc
     5881 ccggggatca aaatcttgaa agtgctttga aatatatcca ggagaacaga aaataaaaga
     5941 attatgtatc taggtacgta attctttata tttatgcttt gtataagcca tattttcaaa
     6001 agttatccac agtcgggttg aaattatttt tgatatatgc aagtattata aatacaaaga
     6061 cctgtaatcc gaactatcac cggatggtgg tgtggctggg acaaccagtc taggatatgt
     6121 ggtcggaaat agccccttgg ggctgttttt tgttatttga acggccactc atcctcgata
     6181 tgttttttta tgatatccct gtaaatggcg tgatcggttt tcttgatgtc gcgatttctt
     6241 cttatgcttc cggc
//
LOCUS       DATKGS010000033        10341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_46292, whole genome shotgun sequence.
ACCESSION   DATKGS010000033 DATKGS010000000
VERSION     DATKGS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46292"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VMQ48_00785"
     CDS             complement(<1..201)
                     /locus_tag="VMQ48_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08609.1"
                     /translation="MKFIIENPKASVANFFRRAGYAFQKQDGDEMAFVRKLTHQPFPR
                     FHLFVKIADYKFQVNFHIDHKAA"
     gene            complement(207..281)
                     /locus_tag="VMQ48_00790"
     tRNA            complement(207..281)
                     /locus_tag="VMQ48_00790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(247..249),aa:Pro,seq:cgg)
     gene            complement(282..887)
                     /locus_tag="VMQ48_00795"
     CDS             complement(282..887)
                     /locus_tag="VMQ48_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08610.1"
                     /translation="MASQIAHVIYAQKYLEKNPRKGGEFFILGTLFPDMRRVAKGMAR
                     KDTHMRFEKIDLDFEGMDLFDAGWKFHLWCDMRREEILNKYNFYTLRGAKAIDPTAKL
                     VEDELVYDKYKNWEKLIFILNNPPIVKTSLDVSQETIEHWYAILAKYFQKKPDDKTMK
                     IFLSKQSSLKGEIAEIIDGVRKLRENQKATDILKKVCEEII"
     gene            complement(902..2461)
                     /locus_tag="VMQ48_00800"
     CDS             complement(902..2461)
                     /locus_tag="VMQ48_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HUD08611.1"
                     /translation="MISKIPKPIIEIIQKLEAAGFEAFVVGGCVRDLLMDAGPKDWDV
                     TTNAKPEEVMKIFPDSFCENAFGTVGVKSEVGVVEVTTYRIESKYSDKRRPDKVEFAK
                     TLEEDLSRRDFTINALALRLNFSKVRPFPSSSKALGIPQGQTLKNSSYEIIDLYDGQK
                     DLKKKIIRAVGDANERFDEDALRMMRAVRFAVTLGGDVKVKRTNGPRKLSKKIPFEIE
                     EKTKKAIEKNAKNLKFIALERIQDELNKILVSDFAAEGIELLRELKLLQYIIPELEKG
                     YGVGQNRHHIYTIYEHSILALKNCPSEKLEVRLATLFHDIAKPETKRGEGAFSTFYNH
                     DHVGARVVEKILKRLKYSSDIIKKVRLLVDNHMFYYNVEEVGASSVRRLVKKVGLGNI
                     DDLIDVRVGDRLGSGVPKAVPYKLRHFKYMVDKISSDPLSVKMLKINGDDLMKGLKLK
                     AGPMLGAILDVLLSEVIENPGENKKEILLKRAKKLSKEDLSKLREMAKEKIEEKKEED
                     DEEIKAKYYVK"
     gene            complement(2544..3095)
                     /locus_tag="VMQ48_00805"
     CDS             complement(2544..3095)
                     /locus_tag="VMQ48_00805"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08612.1"
                     /translation="MDHQIILIDMDGPLADFDGTFLKEWKRRYPEEPHIPLRNRREFY
                     PRDDYPKRLRSKVESIYLDQKFYPKIPPVEEGLAAIREMVDLGISVFICTTPLSLYAY
                     CVLEKYQWVEKYLGYGFTKKLILTRDKTLVRGDMLIDDKPEITGIQKPVWEHIIYDFP
                     YNRHITGNRRIVRWKNWRKILGI"
     gene            complement(3151..3224)
                     /locus_tag="VMQ48_00810"
     tRNA            complement(3151..3224)
                     /locus_tag="VMQ48_00810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3188..3190),aa:Arg,seq:cct)
     gene            complement(3235..3738)
                     /locus_tag="VMQ48_00815"
     CDS             complement(3235..3738)
                     /locus_tag="VMQ48_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08613.1"
                     /translation="MHKIKVLGKYATILNSKRGGSRIRRQKNIELSKIEAREILKSTD
                     RELVIILSMLYWGEGSKKTCDFINSDGRIIEVYLKIIREVFEIEENLIRATMRIFTGM
                     DEKDCLNYWSKITGINKNKFKVRLNDGGTRGKTKYGMCRISIRKGARPLKIIHSLIEQ
                     IHREVCP"
     gene            complement(3925..3995)
                     /locus_tag="VMQ48_00820"
     tRNA            complement(3925..3995)
                     /locus_tag="VMQ48_00820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3961..3963),aa:Ala,seq:ggc)
     gene            4074..5561
                     /locus_tag="VMQ48_00825"
     CDS             4074..5561
                     /locus_tag="VMQ48_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUD08614.1"
                     /translation="MQNAETKLAWLWQNTEKVLIVVFFLTFTFNIRKVFLTPFSFLNG
                     GFNEYTTIFISWADILMIAVILSYTIKWLSGQLVRPASAKFSLYNVIRKKSIVIRNYY
                     GNNVSRETFYLLIFLGWVGLSIFWSQYRLLAVYKFLTIIEIVLFAAVVVKRLKNSDWL
                     KIALFALLLNGLFQSLLGIAQFVHNASLGFHFLGESIIGPNIDGVAKILINGEKHIRA
                     YGTFPHPNILAGFLLIPFFIIIAELVCRIFPFLNKQSLPAGEAEAGERSAQVSHETSL
                     SFVPTNFLWFIFSVTSLGFVLTFSRSAFLGLFFGLLIYIFLIAPTRNLVQKFKSYYKL
                     QVALTILLALMIIALLNGTSFFSNQSLQERQLYQDVSYETISGSPIRGIGAGQFILNE
                     HKLHPDLEAWQYQPVHNVYLLIFSELGIVEFILFILLVVSFLFTNLNDSASRENCLLT
                     KYLFCCIVFSFLVISLFDHYFWDIKQGMFVFALPLIIYYGMIQQK"
     gene            5704..8310
                     /locus_tag="VMQ48_00830"
     CDS             5704..8310
                     /locus_tag="VMQ48_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUD08615.1"
                     /translation="MKIDLRRNTTLASKTPEDGMAQTTTRMPEHQNMRPTSVEQAANR
                     RDTTAPKIMDWIITGAVYLLTFLIPLFVLPNVPSALELNKQVLLVLLGGIAFLAWIGK
                     LAWEGRIRIKKNFLLVPVLLLLIVLSLSTVFSVYRDQSMWGGLGTESLSLVTFIALIA
                     VFLVVNNNFNTREKISNLVLILIGSSFLASIYTILQIFGKFIFKNPVFAQNSFNTIGS
                     VYAFSIFIGAILILTTAVMLEKRPFWIKIFLGLSALIFVFILISINFRTSLIMFLVGM
                     AILLGLAIISSGNEEKNKTLIIPMVILTLVLLSTLIGKSGSIMKVQLPVEVGLSQSAS
                     FDVAKTAWKDKALLGSGLSNYDLNYLRGKPTEINQTDFWSVRFNEAASRFLTLATSTG
                     LLGAAALLFLIGAMLVHMFSSLVKIFEKRDSGVYTLIGITAAWVYLTIAIFFYASNIT
                     LDYSWWILTAGFVTLAAIVFGKKEEIISETSSPRLSLILSFVFVLIIVGFISLIFLEG
                     QKYVAAVAYNQTLASDSQGAKVEDLISKLNRVVNLDPNRDLYHRNLSVALFAFLNQKI
                     SEKGMQNLSENDRADISNLYFAAEDQVKTAVGLNPSNPDNLIQLAQINQNVIGSKDGA
                     DQAALDNYKAALKVSPQDPSIYYQIGQIYLAMADLETAKNASQQKQGTQPTLSDKAKE
                     DIALAKQNFEKAVELKGNYIPAQFMIAVALERLGETDQAIAQLETSQKMNPQDAGISF
                     QLGVLYYQTGKYDQAQTALESTVKLASNYSNAMYFLGLTYDKLNNKKQALEQFQKIAE
                     LNPDNADVKKIVANLQAGKKALDGLDQTTGGQNPNGETSQSQSVQRNQQPVPPNTPID
                     NSQPNQNPNPAP"
     gene            8478..9836
                     /gene="nusA"
                     /locus_tag="VMQ48_00835"
     CDS             8478..9836
                     /gene="nusA"
                     /locus_tag="VMQ48_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HUD08616.1"
                     /translation="MKKTIADDESQTGRLGQFGSAIAQIAEEKGIDKKKVIEAIESAL
                     AAAYKKDYGRRGQHIRAEFDEVTGGSDFYLVKEIVDETTRVFEDDPADAEALAGKPEE
                     NIPEKKAHEEHEESDEENIPRYNPERDLTLEEAQKTKPDAKVGDIIEEKLEAKNDYGR
                     VAAQTAKQVIIQKIREAERDSMYEEYKQKEGEILSGVIQRIEGRNIFLDLGKSTGILF
                     PSEQVEGERYRLGQRLKVYLMKVESDAKGPGIILSRTHPSLVQKLFEIEVPEIFAGTV
                     EIKAMAREAGSRTKIAVSSSEEGIDPIGSCVGQKGTRVQAVIDELGGEKIDIIEWNES
                     PAKFISNALSPAKVLHVELAEEKREARVSVPTDQLSLAIGKQGQNVRLAAKLTGWKID
                     VVGGETKTEEPKKEELEKENAAAAEEKTGKSAESENKAKEKKSDKKKSIKKEKEEKEK
                     NK"
     gene            9857..>10341
                     /locus_tag="VMQ48_00840"
     CDS             9857..>10341
                     /locus_tag="VMQ48_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HUD08617.1"
                     /translation="MNLWHDVSLGEKAPEEFNVIIEIPKGSKNKYEIDKETGLIKLDR
                     AMKSSQDYPFDYGFAPQTLWEDNDALDVVVLTTYPLSTGILVNVRPVAVMHMTDGGES
                     DDKIIAVPMDDPRWENVSDLADINPHTIKEFKHFFETYKSIEEKAVTIDGVEGKDKAI
                     HP"
BASE COUNT         2910 a         2384 c         2027 g         3020 t
ORIGIN      
        1 ggccgctttg tgatcgatat gaaaattcac ttggaattta taatccgcaa tcttcacaaa
       61 aagatgaaaa cgcggaaatg gctggtgcgt gagcttgcgc acgaacgcca tctcatcacc
      121 atcctgcttc tggaaagcat atcccgcccg gcgaaaaaaa ttagccacac tcgccttcgg
      181 attttcaata atgaatttca tatttttggt cgggctggag ggagtcgaac cctctgtctc
      241 acgaacccga atcgcgcgtg ttgccgatac actacagccc gttatattat ctcctcgcaa
      301 acttttttca aaatatccgt tgctttttga ttctctcgca attttcgcac tccgtcaatg
      361 atttcggcaa tttctccctt gagggaagat tgttttgaga gaaagatctt catcgtcttg
      421 tcgtccggtt ttttttggaa atacttggcc agaatcgcgt accaatgttc aatggtttct
      481 tgtgaaacat ccaaactagt cttcactatc ggcggattat tgagaataaa gatcaacttt
      541 tcccaatttt tgtatttatc ataaaccaat tcatcctcaa ccagcttggc cgtcggatca
      601 attgccttcg ccccgcggag agtatagaaa ttatatttgt tgagaatttc ttcccgccgc
      661 atatcgcacc acaggtgaaa tttccatccg gcatcaaata aatccattcc ttcgaagtca
      721 agatctattt tttcaaagcg catatgcgtg tctttgcgcg ccattccttt ggccacccgc
      781 cgcatatccg gaaaaagcgt tcccaaaatg aaaaattcac cgcccttgcg gggattcttt
      841 tcaagatatt tttgggcata gatgacatgt gcgatttggg aagccattat tttagaagat
      901 tttattttac gtaatatttc gccttgatct cctcgtcgtc ttcttctttt ttttcttcaa
      961 ttttttcttt cgccatctcg cggagtttcg acaaatcttc ttttgatagc tttttcgccc
     1021 gcttcagcaa tatttctttt ttattttctc caggattttc aatcacttct gaaagcagca
     1081 catctaggat cgcaccaagc atcggtccgg ctttcaattt caatcctttc atcaaatcat
     1141 cgccatttat cttcaacatt ttgaccgaaa gaggatcact cgatatttta tccaccatat
     1201 atttgaagtg gcggagcttg tatggtacag cctttggcac gcccgatccc aaacgatccc
     1261 caacccgcac gtcaatcagg tcatcgatat ttcccagccc cacttttttc accaatcgcc
     1321 gcacactcga agccccgact tcttcaacgt tataatagaa catgtgattg tcgacaagca
     1381 gccgtacttt tttgatgata tccgaagaat atttcagccg ctttaaaatt ttctccacca
     1441 cccgcgcccc gacatggtca tggttataga aagtcgaaaa ggcaccctca cctctcttcg
     1501 tttcgggttt ggcaatatca tgaaacagcg ttgcgaggcg cacttccagt ttttctgatg
     1561 gacaattttt gagcgccaag atcgaatgtt catagatggt atatatatga tggcggtttt
     1621 gaccgacccc atatcctttt tcaagttcgg gaattatata ttgtagaagt tttagctctc
     1681 tcaatagttc aattccttcg gccgcgaaat ctgaaaccaa aattttgttc aactcgtcct
     1741 gaattctctc gagcgcgatg aatttcaaat tcttcgcgtt tttctcaatc gcctttttgg
     1801 ttttttcttc aatttcgaaa ggtatttttt tgctcaattt cctcggccca tttgttcttt
     1861 tgacttttac gtctccgcca agagtcacgg caaaccgcac cgcccgcatc atccgcaatg
     1921 cgtcttcatc aaatctttcg ttcgcatccc ctactgctcg aataattttc tttttcaaat
     1981 ctttctgccc gtcatacaaa tcgataattt cgtacgaaga atttttcaag gtttggcctt
     2041 gaggaattcc gagagctttg ctcgaggaag gaaaaggccg aaccttagaa aaattcaacc
     2101 gcagcgccaa cgcattgatc gtaaaatccc ttctcgacaa atcctcctcg agtgtcttcg
     2161 caaactcaac cttatccggc cgccgcttgt cggaatattt cgattcaatg cgatatgttg
     2221 ttacttctac aacaccgact tcacttttca cgcccaccgt cccaaacgca ttctcgcaaa
     2281 aactatcagg aaatattttc atcacttcct ccggcttcgc gttcgtcgtc acatcccaat
     2341 ctttcggtcc ggcatccatc agtaaatccc gcacacaccc gcccacgaca aaagcctcaa
     2401 aaccagcagc ttcaagtttc tgaattattt caataattgg ttttggaatt ttggagatca
     2461 tactcttaac ttaaatcaaa acaggtaaaa aatcaaaacg cctctcatat tattgaaagg
     2521 cgttggttat gtttgtggga agtttatatt cctagaattt tcctccagtt tttccaccgc
     2581 acaattcttc tattgccggt gatatgcctg ttatacggaa aatcatagat gatatgctcc
     2641 caaacaggtt tttggatgcc ggtgatctcc ggtttgtcgt caatgagcat atcacctcgg
     2701 acaagagttt tgtcgcgggt gagaatcagt tttttcgtaa aaccatagcc caaatatttc
     2761 tccacccatt gatatttttc caacacgcaa taggcataaa gagaaagtgg cgtcgtgcag
     2821 atgaaaacgc tgatacccaa gtcgaccatc tcacgaattg ccgccagtcc ttcctcgact
     2881 ggcggaattt tgggatagaa tttttgatcc agatatatcg attccacctt ggatcgtagt
     2941 cttttgggat aatcatcccg aggatagaat tctcttcgat tccgaagcgg gatatgcggt
     3001 tcttctggat accttcgctt ccactccttc aagaatgttc catcaaaatc cgccaacgga
     3061 ccgtccatat caataagtat tatttggtga tccattttat ccccctttga attctcaaaa
     3121 tattttaatt ttcaaaaaac ttcatttttg tcccctcgag aggagtcgaa cctctagcct
     3181 ttcccttagg acgggaccgc tctaatccat tgagctacga gggggcacac ttgttcaagg
     3241 acagacttct ctatgaattt gctctattaa tgaatgaata attttcagag gtcttgctcc
     3301 ctttctaatt gatattctgc acatgccata ttttgttttt ccccgtgtgc cgccgtcatt
     3361 aagccgaact ttaaatttat tcttgtttat cccagtaatt tttgaccaat agttcaaaca
     3421 atctttttca tccatgcctg taaatattcg catagttgcc ctgatcaggt tttcttcaat
     3481 ttcaaatact tctcttatta tctttagata aacttcaata attcttccat ctgaatttat
     3541 aaaatcgcac gttttcttgc taccttcacc ccagtacagc atagacaaaa taattaccaa
     3601 ttccctgtca gtacttttca atatttctct cgcctcaatt tttgaaagtt cgatattttt
     3661 ttgtcttcta attctgctac cgcccctctt tgaatttaaa attgtggcgt actttcccag
     3721 tactttgatt ttatgcacat gatgccagac agttgttttt ggaatatgca aactgtcgac
     3781 cagttcattg atactgtatc cttgttttcg caatagtatt aattttttta ttttttctgt
     3841 ggaatgtatc ctcacgcatc aagtgtacca cacactagac ttttcaacaa tagcctagta
     3901 ggaaaaagta agttcttcta tttgtggacc cacggggaat cgaaccccgg tccgatccat
     3961 gccatggatc tgttctacca ttatactatg ggcccactac actttaatat attatgtata
     4021 tttcatatat tagtcaattc actcgcttta ttgtttcatg ctaaactgtg tctatgcaaa
     4081 acgcagagac aaaattggcc tggctctggc aaaacacgga aaaagtcctg atcgttgttt
     4141 ttttcctaac tttcacgttc aatatccgca aagtcttttt gacgcctttt tcctttctga
     4201 atgggggatt taatgaatat acgaccattt tcattagttg ggcggatata ttgatgattg
     4261 ccgttattct tagttatact ataaaatggt tatctggtca attagtaaga ccagcctccg
     4321 ctaaattctc attatacaac gtaatacgca aaaagtcaat cgtaatacgg aattattacg
     4381 gcaataatgt ttcacgtgaa acattttatt tactgatctt tctcggttgg gtcggattat
     4441 ccatattctg gtcgcaatac aggcttctag ctgtttataa atttttgaca atcatcgaaa
     4501 tcgtgctctt tgccgccgta gtcgtaaaac gcctaaaaaa cagcgactgg ctgaaaatag
     4561 cccttttcgc gctcctttta aatggcctat tccaatctct gctgggaatt gcacagtttg
     4621 tccacaacgc ttccctggga ttccattttc tcggcgagag catcatcgga ccaaatatcg
     4681 acggagtggc aaaaatccta atcaacggcg aaaagcacat tcgagcttat ggaacatttc
     4741 cacatccaaa tattttggct ggattcttgt taattccctt tttcataatt attgcggagt
     4801 tagtttgcag aatattccct tttcttaata agcagagcct gcctgccggc gaggcagagg
     4861 ctggggagag gtcggcacaa gtttcacatg aaacaagtct aagcttcgta ccaacgaatt
     4921 ttctttggtt catattctcg gtaacgagcc ttggttttgt tttgacattt tctcgctctg
     4981 ctttcctggg tttgtttttc ggattgctaa tttatatttt tttgattgct cctactcgaa
     5041 atttggttca aaaatttaaa agctattata aacttcaagt tgccttgaca atccttctag
     5101 ccttgatgat tattgcttta ttgaatggaa cttccttctt ttcaaaccaa tcgcttcaag
     5161 aaaggcagct ctatcaagat gtttcatatg aaacaatttc aggcagtcct atccgaggaa
     5221 tcggtgcggg acaatttatt ttaaacgaac ataaattgca cccggattta gaagcctggc
     5281 aatatcaacc agtgcacaat gtttatcttt tgatatttag cgagttgggt atcgttgaat
     5341 ttattttatt tattctcctt gttgtttctt tcctatttac aaatttgaat gatagtgcaa
     5401 gtagagaaaa ctgtctattg actaaatact tattttgttg catagtattt tcttttcttg
     5461 tcatttcact tttcgatcat tatttctggg acatcaagca aggcatgttt gttttcgccc
     5521 tgcctctgat aatttattac ggcatgattc aacaaaaata aaaattagac aaggaattat
     5581 aatctgctat aatcaaaaag tattaaactt aattcacccc aagcccattt ttcagctcaa
     5641 ttaaattagg ctgattcgca gcaacaatga gagggcggaa ttggttcggg gcaaaacaga
     5701 aaaatgaaaa tcgacttgag aagaaacact actcttgctt caaaaacacc agaagatggt
     5761 atggcacaga ctaccacgcg gatgcctgaa catcaaaata tgcggccaac cagcgtagaa
     5821 caagcagcca atcggcgcga cacaaccgcg ccaaaaataa tggactggat tatcaccggg
     5881 gcagtctatc tcctcacctt tttgatcccc ttgtttgtcc ttccgaatgt cccaagcgcc
     5941 ttggaactca ataagcaggt actactagtg ctgctcggcg gcatcgcctt tcttgcctgg
     6001 attggaaagc tggcgtggga gggaagaatt cggatcaaga aaaatttcct tttggtccct
     6061 gtcttgcttc ttcttatcgt tctctcgctt agcacggttt tctccgtata tcgcgaccaa
     6121 agtatgtggg gaggactggg tactgagagt ctttctttgg taacctttat tgctttgatt
     6181 gccgtatttt tggtagtcaa caacaatttc aatacccgtg aaaaaatcag caacttggtt
     6241 ctcattttga tcggatcatc ttttctggct tcaatatata caatattgca aattttcgga
     6301 aaattcatct tcaaaaatcc ggttttcgct caaaattcgt tcaacacaat cggatccgtc
     6361 tatgcttttt ctattttcat tggcgctatc ctgatcttga caactgctgt gatgcttgag
     6421 aaaagacctt tctggatcaa gatttttctc ggcctttcag cgttgatctt tgttttcatc
     6481 ctgatttcca tcaatttccg taccagtctt atcatgtttc tcgtgggaat ggccattctc
     6541 ttgggtcttg ccataatttc aagcggaaat gaagaaaaaa acaaaaccct tattattccc
     6601 atggtaattt tgacccttgt tttgctcagc actttgatcg gaaaatccgg atccatcatg
     6661 aaagttcagc tcccggtcga agtcggtctt tctcaaagcg cttcatttga tgttgcaaaa
     6721 acggcttgga aagacaaggc tcttcttgga tcagggcttt caaattacga tctcaactat
     6781 ctccggggaa aaccgactga aataaatcaa actgattttt ggtcggtaag attcaacgag
     6841 gctgccagcc gcttcctcac actggcaact tccacgggac ttcttggcgc tgccgccctc
     6901 ttgtttttga tcggagcaat gctagtgcat atgttctcat ctctcgtcaa aatatttgag
     6961 aaaagagata gtggagttta tacattgatc ggcatcacgg ccgcctgggt ctatttgact
     7021 attgccattt tcttctacgc ctcgaacatc actctcgatt attcctggtg gatactgacc
     7081 gccggtttcg tcaccctcgc tgcgatcgtt ttcggcaaaa aagaagaaat catcagtgaa
     7141 acatcttctc ctcgattgtc actcatcctc tcatttgtct tcgtgctcat catagtcggc
     7201 ttcatttcct tgatcttcct tgaggggcag aaatatgtgg ccgctgttgc ctacaaccag
     7261 actctcgcca gcgactccca aggcgccaaa gtggaagacc tgatttccaa attgaaccgg
     7321 gtggtaaact tggatccaaa ccgcgacttg tatcaccgaa atctttctgt tgctctcttt
     7381 gccttcttga accaaaaaat ttccgaaaaa ggtatgcaaa atttgtctga aaatgaccgg
     7441 gcagacatca gcaatttata ttttgccgcg gaagatcagg tgaaaacagc tgtgggcttg
     7501 aaccctagca atcctgacaa cttgattcaa ttggctcaaa tcaaccagaa tgtgatcggg
     7561 tcaaaagatg gcgctgatca agcggctctc gacaattata aagccgcgct caaggtcagt
     7621 ccgcaggatc cttcgattta ttatcaaatc ggccagattt accttgcaat ggccgacctc
     7681 gaaaccgcta aaaacgcctc ccagcagaag cagggaactc agccgacact ttccgacaaa
     7741 gctaaggaag atattgctct tgccaaacag aattttgaga aagcggttga actcaagggc
     7801 aactatattc cggctcaatt catgatcgcc gttgctttgg aaagactggg tgaaacggac
     7861 caggctattg cgcaactcga aacaagccaa aaaatgaatc ctcaagacgc tggcatttcc
     7921 ttccagcttg gcgttctcta ctatcagacc ggcaagtatg accaagccca aacagctttg
     7981 gagagcaccg taaagctggc cagcaactat tccaatgcga tgtatttcct cgggctcact
     8041 tatgacaagc taaacaacaa gaagcaggcg cttgagcaat tccagaaaat tgccgagttg
     8101 aatccggata acgcggatgt gaaaaaaatt gttgccaatc ttcaagccgg caaaaaagcg
     8161 cttgacgggc tggaccaaac tacgggtggc cagaatccga atggcgaaac ttcgcaaagt
     8221 cagtctgttc agcgcaatca gcaacctgtt ccacccaata ccccgatcga taacagtcaa
     8281 ccaaatcaga atcctaatcc agctccctaa atcttccttc aaaaatccct cgcaaaccag
     8341 cgagggattt ttattatttg acaagaaaac aatcgcctgc tatattgtaa tcacctgaag
     8401 tgatccgaga tgtccgtgcg aacgggcatt ttatattaat tttaacgtac tatattatta
     8461 tggccagacc aaaaaaaatg aaaaaaacta tcgcagatga tgaatcacaa accgggcgac
     8521 tcgggcaatt tggaagcgcg atcgcacaaa tcgccgaaga aaaagggatc gacaagaaaa
     8581 aagtaattga ggcgatcgaa tcggcgctag cagctgcgta caaaaaagat tatggccgac
     8641 ggggacagca tatccgcgcc gaatttgacg aagtcaccgg cggatccgat ttctatctcg
     8701 taaaagaaat tgtcgacgag acaactcgcg tttttgaaga tgatcccgcc gacgctgaag
     8761 ctttggcggg caagccggaa gaaaatatcc cagagaaaaa agcgcacgag gaacatgaag
     8821 aatccgacga agagaatatc ccgcgataca atccggaaag agatctgact ctcgaagaag
     8881 cgcagaaaac aaaacctgac gcaaaagtgg gggatatcat cgaagaaaaa ttggaagcca
     8941 aaaatgacta tgggcgcgtt gcggcccaga ccgccaagca ggtcatcatc caaaagattc
     9001 gcgaagcaga acgtgattcg atgtatgaag aatacaagca aaaagaaggc gagattttga
     9061 gcggagtaat ccagcggatt gaaggacgca acatattcct tgatcttgga aagtcgaccg
     9121 gaattttgtt tccgtcggag caggtggaag gcgagagata ccgcctcggc caacgcctca
     9181 aggtatatct gatgaaggtt gaatccgacg ccaaggggcc gggaatcatt ctttcccgaa
     9241 cccacccgtc gcttgtgcaa aaactttttg aaattgaagt tccggaaatt ttcgcgggaa
     9301 cagttgaaat caaagctatg gcgcgcgaag ccgggtcacg cacaaagatc gccgtatcct
     9361 caagcgaaga gggcattgat ccgatcggat cttgcgtggg gcaaaaagga acacgcgttc
     9421 aggctgtgat cgatgagctg ggtggagaaa aaatcgatat cattgaatgg aacgagagtc
     9481 ccgcaaaatt catttcaaac gcgctctcgc ccgcaaaagt tctgcacgtg gaactcgctg
     9541 aggaaaaaag agaagcccgt gtttccgtcc cgaccgacca gctctctctg gccatcggca
     9601 aacaaggaca aaacgtgaga ttggcggcga aactgaccgg ctggaaaatc gacgttgttg
     9661 gcggagagac aaaaacggaa gaaccaaaga aagaagaatt agaaaaagaa aacgcagctg
     9721 ctgctgaaga aaaaacggga aaatctgccg aatctgaaaa taaagcaaaa gaaaaaaaat
     9781 ctgacaagaa aaagagtatt aaaaaagaaa aagaagaaaa agaaaaaaat aaataatttt
     9841 taatttaaaa tttccaatga acctatggca tgacgtctcc cttggagaaa aagcccctga
     9901 agaattcaat gtaatcattg agattccaaa gggatcaaag aacaaatatg aaattgacaa
     9961 agaaaccggc ttgatcaaac ttgatcgcgc catgaaatct tcccaggatt atccgtttga
    10021 ttatggtttt gcgccgcaga cactttggga agacaacgat gcattggatg tggtcgtgct
    10081 cacaacatat cccttgagca ccggcatact tgtcaatgtg aggccggttg cagtgatgca
    10141 tatgactgac ggcggagaat cagacgacaa aataatcgcc gttcccatgg atgatcctcg
    10201 ctgggaaaat gtaagtgatc ttgctgatat taatcctcat acaataaagg aattcaagca
    10261 tttctttgag acatacaaat caattgaaga gaaagccgta acaattgacg gcgtagaagg
    10321 aaaggacaaa gcgatccacc c
//
LOCUS       DATKGS010000034         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_3698, whole genome shotgun sequence.
ACCESSION   DATKGS010000034 DATKGS010000000
VERSION     DATKGS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3698"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /gene="infC"
                     /locus_tag="VMQ48_00845"
     CDS             <1..552
                     /gene="infC"
                     /locus_tag="VMQ48_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HUD08618.1"
                     /translation="YIRRRFFHRRPQEFRGPRVRINEFIRISPVRLIDDTGQMVGVVP
                     TVEALQMAKEKELDLVEIVPNAKPPVVKIIDYGKYQYQKAKEDQQQKANQKKTEIKGL
                     RIGLKTDDHDIEVRHKQAEKFLTQGNKVKIEIRLKGREKAFQPLAREALQNFVKSISV
                     PNKLEQEIKRFPGGFNVLIIPDK"
     gene            557..757
                     /gene="rpmI"
                     /locus_tag="VMQ48_00850"
     CDS             557..757
                     /gene="rpmI"
                     /locus_tag="VMQ48_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HUD08619.1"
                     /translation="MPKLKTRKAAAKRVKITAKKKLMRRETGQGHFNARETGQATRSK
                     RDDSQVFKTDAKNILLNLPYGN"
     gene            780..1124
                     /gene="rplT"
                     /locus_tag="VMQ48_00855"
     CDS             780..1124
                     /gene="rplT"
                     /locus_tag="VMQ48_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HUD08620.1"
                     /translation="MSRVKRGVAASKRRKNVLKDAKGYMWHRKSAFRAAKEALLKAGK
                     YAYRDRRVKKRTFRRLWITRLNAALRMHGEKYSAFIAKITDKKIEVDRKVLSEMAANF
                     PEHFKSFVEKVK"
     gene            complement(1482..1895)
                     /locus_tag="VMQ48_00860"
     CDS             complement(1482..1895)
                     /locus_tag="VMQ48_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HUD08621.1"
                     /translation="MNLTSSPAKGRCPKGGGVFAMKRFNNLNWYKNLRKGLRNNVTPQ
                     EIILWSRLRRSQLGFKFRRQHSIGKYIVDFCCPSKNLIIEVDGGQHDEANQRRYDENR
                     TKYFESLGFKVLRFWDNDVNNNLSSVIEEIINNLG"
     gene            complement(2024..>2443)
                     /locus_tag="VMQ48_00865"
     CDS             complement(2024..>2443)
                     /locus_tag="VMQ48_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HUD08622.1"
                     /translation="SAYNIGSIFRTADGAGVAKIYLTGYTPAPPHAGIAKTALGAEKF
                     VDWEKISKLGTAIKKLKKEKYELAAVEQNTRSTSYDFFKPKGKVALILGNEVRGLSAA
                     ILQKCDKIIEIPMRGEKESLNVSVAFGIAVYSTISRQ"
BASE COUNT          706 a          604 c          479 g          654 t
ORIGIN      
        1 tatataagac gaagattttt tcaccgccgt ccgcaggaat ttcgcgggcc gcgcgttcgg
       61 atcaatgaat tcatcagaat atcccccgtc cgactgatcg acgacactgg ccaaatggtc
      121 ggtgttgttc ccacggttga ggctcttcaa atggccaaag aaaaagaatt ggatctcgtt
      181 gaaattgttc ccaacgccaa gccgccggtc gtaaagatca ttgactacgg aaaatatcag
      241 taccaaaaag ccaaagaaga ccagcaacaa aaagccaatc agaaaaagac cgagatcaaa
      301 ggactgcgca tcggcctcaa aaccgacgac cacgatatcg aggtccgcca caaacaagcc
      361 gaaaaatttc tcactcaggg aaacaaagtg aaaatcgaga ttcggctcaa gggacgtgag
      421 aaggcttttc agcctctcgc ccgggaagcc ctccaaaatt tcgtcaaatc aatttcagtc
      481 ccaaacaaac ttgaacaaga aatcaaaaga ttccccggag gattcaatgt actcataatt
      541 cctgataaat agaacaatgc caaaactcaa gaccagaaaa gccgctgcaa agcgcgtcaa
      601 gatcaccgcc aagaaaaagc tcatgcgccg ggaaaccggg cagggccatt tcaacgcgcg
      661 cgaaaccgga caagcgaccc gatcaaagag agatgactcc caggtattca agacggacgc
      721 gaaaaatata ttattaaact tgccatacgg aaactaattt caaataatca ctgaaaaata
      781 tgtcacgagt caaacgcggt gttgccgcct ccaaaagaag aaaaaatgtt ttgaaagacg
      841 ccaagggtta tatgtggcac cgcaaatcag ctttccgcgc cgccaaagaa gccctgctaa
      901 aggccggtaa atacgcctat cgcgaccgcc gggtgaagaa acgaaccttc cgccgcttgt
      961 ggatcacgag actcaatgcc gcgcttcgaa tgcacggaga aaaatacagc gcgttcatcg
     1021 cgaaaataac ggacaaaaag attgaagtcg accgcaaggt cctttccgaa atggccgcca
     1081 atttccccga gcatttcaaa agttttgtgg aaaaagtgaa atagatatct tgctatatta
     1141 taaaaaaatc ccggtcatac cgagattttt tcttttcgaa gcaacctctt tttttctttc
     1201 tccccttttg cataaccgcc gatttggccg tcggagcgga tgacccgatg acacggaatt
     1261 gttggttttt tattttcaac gcaatcgcta taacacttat tcaaaatatt ccctatcgcg
     1321 cgggcggctt tttcgttacc ggattttttc gcaacctctt tgtatgtcaa agtttttcct
     1381 ttggggattt gagaaactag gcggatgact ttttgagtga aagtggacat accatttaac
     1441 tatcctcccc tcctgccaag gaggggataa gaggggtggt tttatcccag attattgatt
     1501 atttcttcaa tcacactact caaattatta ttaacatcat tgtcccagaa tctcagcacc
     1561 ttaaagccta aactttcaaa atattttgtt ctattttcat catatcttct ttgatttgct
     1621 tcgtcgtgct gtcctccatc aacttcgatt atcagattct tactagggca acaaaaatca
     1681 actatgtatt tcccaattga gtgctgcctt ctgaatttga atcccaattg acttcttctc
     1741 aatcttgacc aaagaataat ttcttgcggg gtcacattgt tgcgaagacc ttttcgcaaa
     1801 tttttgtacc aattgagatt attgaatctt ttcattgcaa aaacccctcc gcccttcggg
     1861 cacctcccct tggcagggga ggaggttaaa ttcatttttt ctcgctcgca aaaatatccc
     1921 ccaatagttc cccaccgagt ttatatatcg cgtaaaaagg caaaaacacc cagataaatt
     1981 ttttgatcca ccactcaatg aatttttcca tatatttttt cgattattgc cggctgattg
     2041 ttgaatacac cgcgatcccg aacgcgaccg aaacattcaa tgattctttc tcgcctcgca
     2101 tgggaatttc aattatcttg tcgcattttt gaaggatcgc cgcgctcaat cccctcactt
     2161 cgtttcccag gatcaaagca acttttcctt ttggcttgaa aaaatcgtat gacgtgctcc
     2221 tggtgttctg ctcaactgcc gccaattcat atttttcttt tttcaatttt ttgatggccg
     2281 tgccaagttt tgaaattttc tcccaatcga caaacttttc cgctccgagc gcggtttttg
     2341 caatgccggc atgaggcggg gctggcgtgt atcccgtgag ataaattttc gccacccccg
     2401 ccccatcggc ggttcgaaaa atagatccga tattataggc gct
//
LOCUS       DATKGS010000035         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_74384, whole genome shotgun sequence.
ACCESSION   DATKGS010000035 DATKGS010000000
VERSION     DATKGS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74384"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..>2164
                     /gene="secA"
                     /locus_tag="VMQ48_00870"
     CDS             135..>2164
                     /gene="secA"
                     /locus_tag="VMQ48_00870"
                     /inference="COORDINATES: protein motif:HMM:NF006630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HUD08623.1"
                     /translation="MIFGKLFGSAEKQTNKFLPLVDEINGLEAEMEKLSDEQLKAKTE
                     EFKKRLENSEKEDDMLPEAFAVVREAVKRVDGKRLFDVQLLGGITLHQGKIAEMKTGE
                     GKTHTATTAVYLNALSGKGVHIVTVNDYLSKRDANWMGPIYNFLGLSCTCIQHDASFL
                     YEPNVKDSDEVSVEMENLRPVSRREAYAADITYGTNNEFGFDYLRDNMVQSLDQMVQR
                     QEMNYAIVDEVDSILIDEARTPLIISAPDSESTKLYKQFAQIVPRLKGEEDYTVDEKM
                     KAVSLTDKGISAIEKMLGIENIYDVSKISYVHQLEQALKANILFKRDRDYVVQDEQVV
                     IVDDFTGRLMPGRRYSEGLHQAIEAKENVEVQKESRTLATITFQNYFRMYAKLAGMTG
                     TALTSAEEFAKVYKLDVVPIPTNQPMIRKDLADTVYKNEKGKFNAVVEEIKMRHEKGQ
                     PVLVGTIAIERSELLHDMLERTGVPHEVLNAKNHEKEAQIIARAGEKGAVTIATNMAG
                     RGTDIKLGEEVRELGGLHILGTERHEARRIDNQLRGRAGRQGDPGSSQFFVSLEDELM
                     RRFGGDKVKNIMDRFGLPEDQPIENKIISRSIESAQSKIEGFNFDIRKHVLEYDDVMN
                     KQREVIYKRRREILEKYSLKDDVMDIIEEEIEQIVSMHTAGHPEDWQMEDIL"
BASE COUNT          670 a          468 c          569 g          457 t
ORIGIN      
        1 taatgtttaa aatattaagt cattagaatt tgattcaaaa ttcaaaactc gaaattcaga
       61 attagaaact ttgcaatgta tttactaaat atgcaaaatg atataagctg aatagcgtaa
      121 taaagtgata aaatatgata tttggcaaac tgttcggctc ggcggaaaag caaaccaaca
      181 aatttctgcc gcttgttgat gaaataaacg ggcttgaggc ggagatggaa aagctttccg
      241 acgagcaact caaggcgaaa accgaagaat tcaaaaaaag gcttgagaat agcgaaaaag
      301 aggatgatat gctccctgaa gcttttgccg tggtccggga agcggtgaaa agagtggatg
      361 ggaaaaggct tttcgacgtc cagcttttgg gcggaatcac acttcatcag ggaaaaatcg
      421 ccgaaatgaa aacaggggaa ggcaaaacgc acacggcgac aacggcggtc tatctcaacg
      481 ctctctccgg aaaaggcgtg catattgtga cggtcaacga ttatctttcc aagcgtgatg
      541 cgaattggat ggggccgatc tacaattttc tcgggctctc ttgcacgtgc atccagcatg
      601 acgcttcttt tctctatgaa ccaaatgtca aagacagcga cgaagtgagc gtcgaaatgg
      661 aaaatttgcg gccggtttca cggcgcgaag catacgcagc cgatatcacc tacggtacga
      721 acaacgaatt cgggttcgat tatctgcgtg acaatatggt gcaaagcctc gatcaaatgg
      781 ttcaacggca ggagatgaat tatgccattg tcgatgaagt ggactcaatc ctcatcgatg
      841 aagcccgtac gccgctcatc atcagcgcgc cggacagcga atcaacgaag ctctacaaac
      901 aatttgccca aatcgtaccg cgcctcaagg gagaggagga ttatacagtc gatgaaaaaa
      961 tgaaggcggt atcgctgaca gacaaaggga tatcggcgat cgaaaaaatg cttggaattg
     1021 aaaatatata cgatgtctcc aaaatttcct atgtccacca gcttgagcag gcgctcaagg
     1081 ccaatattct tttcaagcgc gaccgggact atgtcgtgca ggacgaacag gtggtcattg
     1141 tggacgactt cacgggacgg ctgatgccgg gacggcgcta ttctgaagga ctccatcagg
     1201 cgatcgaagc caaagaaaac gtggaagtac aaaaagaatc gcggacgctg gcgacgatca
     1261 ctttccagaa ctattttcgc atgtacgcga aacttgccgg aatgaccgga acggcactca
     1321 ctagcgcgga agagttcgca aaagtataca aattggacgt ggttccgatt ccgaccaatc
     1381 agccgatgat ccgcaaagac ctggcggata cggtctataa aaacgaaaaa ggaaaattca
     1441 acgcggtggt cgaggaaatc aaaatgcggc atgaaaaagg ccagccggtt cttgtcggca
     1501 cgattgccat tgaaagatca gagctccttc atgatatgct cgagcggacc ggtgtgccgc
     1561 atgaggtttt gaatgccaaa aatcacgaaa aagaagccca aatcatcgcg cgggccggcg
     1621 aaaagggcgc ggtgacgatt gcgacaaaca tggccggccg cgggacagac atcaagctgg
     1681 gtgaggaagt ccgggaacta ggcggactcc atattctcgg gacggaacgt cacgaagccc
     1741 gtcggatcga caatcagctt cgcggacgag ccggacggca gggcgatccg ggatcatcgc
     1801 aattttttgt ttcgctcgaa gacgagctga tgcgtcgttt tggaggcgac aaggtgaaaa
     1861 atatcatgga tcgtttcgga cttcctgaag accagccgat cgagaacaaa atcatttccc
     1921 gttcgatcga gtcggctcaa tcgaaaattg aaggcttcaa ctttgatatc cgcaaacacg
     1981 tccttgaata tgatgatgtg atgaacaagc agcgcgaagt gatatacaaa cggcggcgcg
     2041 aaattttgga aaaatattct ctcaaagatg atgtgatgga tatcattgaa gaagaaatcg
     2101 agcagatcgt cagtatgcac acggcgggac atccggaaga ctggcaaatg gaagatattt
     2161 tgaa
//
LOCUS       DATKGS010000036         5038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_205098, whole genome shotgun sequence.
ACCESSION   DATKGS010000036 DATKGS010000000
VERSION     DATKGS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205098"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VMQ48_00875"
     CDS             <1..715
                     /locus_tag="VMQ48_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HUD08624.1"
                     /translation="KADGAESKKIFFTTEFFRDPTPKIDEGSDPSVFIKSGKIVCKDK
                     AGTEKDICDLNDIKLRGGHNIGNVLASVAAVSAFGIAAEKICEAIKNFKGVPHRLELV
                     REIDGVKYVNDTAATMPDAAIAGINSFSENIILIAGGMDKNLDFKEFAKAVSEKVKKM
                     ILLKGDATEKMKTELKKIDAENKIDSEFDNLKEALLRAKSLAGPGDIILFSPGATSFN
                     MFKNEIDRGDKFREAVESI"
     gene            712..1551
                     /locus_tag="VMQ48_00880"
     CDS             712..1551
                     /locus_tag="VMQ48_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08625.1"
                     /translation="MNKLAEKLRNKPIVIDQWAYKMVGKRNEGPFKRLYQELKVLSDR
                     DKKNDELIDISKIGIVSRDYYFERCAGAGNGYNKLFAKGPIFPSVLLRKSHAKRLSRA
                     DCYLRKMGLFLNVVSGWRHPELQKMIKDEYAKKHGKDTADRLFASINGKVPAPHSTGA
                     AFDAEVRHLESGEVLNTIVKYRGEKINSIYWGEELLKERKLKEKANQEAALNRRILYH
                     ILCTKDVLFSSRKDLFFVHPGEYWHYGEGETLSSFLTGKKSIKHGLIYPVFNGKKNNP
                     DIE"
     gene            1556..2713
                     /locus_tag="VMQ48_00885"
     CDS             1556..2713
                     /locus_tag="VMQ48_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUD08626.1"
                     /translation="MSKKKKKLYSNEVVTPICKSATYYFEDTKEVIKYHKGKISAGRY
                     GRYDNPNWIEVEDKLAKLDDFDAALLFPSGMSAIATTFLSFLQNKDKVIYTGKGYRNI
                     RSLCGNILSKFGVESTSLSPANPERFLKELSHFYDKKTKIVFLEIPSNPHLHMVDLES
                     VRKILRKDTILIVDSTFATPINFSPKKWGADLVIHSCGKYLGGHADIMAGSVAGSKEK
                     IEIIRNYRNVMGCISGSENAFLLNRSLATLKLRMDYLNKNGMKLAKYLNDNPNIKKVY
                     YSGLPSHSSHELAKKYLSGHGGVVSFELEASISQTAKFVELLKIPFMGTNFGSNYSMV
                     EQCAIFTYFKETKKTKKDLGISDSLIRYSIGFEDIDIIIADIEQAIRTTFK"
     gene            3196..4633
                     /locus_tag="VMQ48_00890"
     rRNA            3196..4633
                     /locus_tag="VMQ48_00890"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1580 a          917 c         1238 g         1303 t
ORIGIN      
        1 gaaagcggat ggggcggagt cgaaaaaaat atttttcaca acggaatttt ttagggatcc
       61 gacccctaaa atcgacgagg ggtcggatcc ctccgttttt attaaaagcg gaaagattgt
      121 ttgtaaagat aaagcgggta ctgaaaaaga tatctgcgac ttgaacgaca tcaaattgcg
      181 gggcgggcac aatatcggga atgtattggc atctgtggca gcagtttcag cttttggtat
      241 cgcggcggaa aagatctgcg aggcgatcaa aaatttcaaa ggcgtgccgc atcgactgga
      301 attggtgcgt gaaattgatg gcgtgaaata tgtgaacgac acggcggcga cgatgcccga
      361 cgcggccatt gcagggatca attctttttc ggaaaatatt atcctgatcg ccggcggaat
      421 ggacaaaaac cttgatttca aggaatttgc gaaagcggtc agtgaaaaag tgaaaaaaat
      481 gattttgctg aaaggagatg cgacggaaaa aatgaaaaca gagctcaaaa aaatcgatgc
      541 tgaaaacaaa atcgacagcg aatttgataa cttgaaagaa gcgcttctcc gcgccaaaag
      601 cctcgctggt cctggggata ttattctttt ttctccgggg gcgacgagtt tcaatatgtt
      661 caaaaacgag attgaccgag gagacaagtt tcgggaggct gtggaatcaa tatgaataaa
      721 cttgcggaaa aattgagaaa taagccgata gtcattgatc agtgggcgta caagatggtt
      781 ggcaagcgaa atgaaggtcc attcaagcgt ttgtatcaag aacttaaagt gttgtctgat
      841 cgcgacaaaa aaaacgatga attgatagat atttccaaaa ttggaattgt cagcagagat
      901 tattattttg aaagatgcgc tggtgcggga aacggatata acaaactgtt tgcgaaaggg
      961 ccgatctttc cgagtgtttt gttgagaaaa tcccacgcaa aaagactatc gagagcagat
     1021 tgctatttac gaaaaatggg actttttctg aatgttgttt ccggttggcg ccatcctgaa
     1081 ttgcaaaaaa tgattaaaga cgaatatgcg aaaaaacatg ggaaagatac tgccgacaga
     1141 ttatttgcgt ctattaatgg aaaagttcct gctcctcatt cgactggtgc tgcttttgac
     1201 gcagaagttc ggcatttgga aagtggagag gttttaaata caattgtgaa atataggggc
     1261 gaaaagatca atagcattta ttggggagag gaattgctaa aagagagaaa actgaaagaa
     1321 aaagcgaatc aagaagccgc attaaatcga agaatcctgt atcatatttt gtgtacaaaa
     1381 gatgttttgt tttcttcgag aaaagatctt ttttttgttc atccggggga atattggcac
     1441 tatggggagg gagaaactct gagttctttt ttaacgggaa aaaaatcaat aaagcatggc
     1501 ttgatatatc ccgttttcaa tggtaagaaa aataatcctg atatcgaata aaaaaatgag
     1561 caagaagaag aaaaaattat attcaaatga agtagtgacc ccgatttgca aatcggcaac
     1621 ctactatttt gaggatacta aagaagtgat taaatatcac aaaggcaaga taagcgctgg
     1681 caggtatgga aggtatgaca atccaaattg gattgaagtt gaagataagc tggcaaaatt
     1741 ggatgatttc gatgcagccc ttctctttcc atctggcatg agcgcgatcg caactacatt
     1801 tttgagtttt ctccaaaaca aagataaggt tatttatact ggcaaaggat acagaaatat
     1861 ccgaagctta tgcggaaata ttctttcaaa gtttggtgtt gaatcaactt cactttctcc
     1921 tgcaaaccca gaaagatttc taaaagaatt gagtcatttt tatgacaaaa aaactaaaat
     1981 tgtattcttg gaaatacctt caaatccgca tttgcatatg gtcgatcttg aatctgtgag
     2041 gaaaatacta cgaaaagata caattcttat tgttgatagc acgtttgcta ctccaatcaa
     2101 tttttcgccc aaaaaatggg gagcagatct tgtgattcac agctgcggaa aatatcttgg
     2161 aggacatgct gatattatgg cgggtagtgt agctggatca aaagaaaaaa tagaaattat
     2221 tcgaaattac agaaatgtta tgggatgtat atctggttca gaaaatgctt ttttgttaaa
     2281 tcggagtctg gctacgttga aattgagaat ggattatttg aataaaaatg gaatgaaact
     2341 tgcaaaatac ttaaatgaca acccgaatat taaaaaagta tattatagcg gacttccctc
     2401 tcattccagc cacgagctag caaaaaaata tctcagtggt cacggaggag tggtatcatt
     2461 tgaacttgaa gcttcaatat cccaaaccgc caaattcgtg gaattattga aaattccgtt
     2521 tatgggtact aattttggat caaactattc aatggtcgaa cagtgtgcaa ttttcacata
     2581 tttcaaagaa acgaaaaaaa caaaaaaaga tttgggaatc agcgattcat tgattcgcta
     2641 tagtataggt tttgaagata ttgatattat catagctgat atcgagcaag cgataagaac
     2701 gactttcaaa taattcttcc agccaagagc attggtgaat tcaagaattt agagcccctt
     2761 gacacgcttt ttcagcctgc tataattatt cttgccgtta aaattaaatt caaattcaaa
     2821 aaacagtcga tcatttgaag cacaactagg gtgaaccaaa atgaaagacg cgactagaat
     2881 aggttgcaat ttgtttgcag gttattccgg tcgtgttttc gttgtccgcc gaagaggcgg
     2941 gaattgacaa caataagcgc gatgaacctt aacaatttat tgacaacatt tcccgatgca
     3001 acgtcgggaa ataacccgcc tcatcggcgg gtaaaattgc aaatttttgt ggttgtcctg
     3061 accccgatgc aagtcggggg ctaagagtct attcaaatca gaaattagtt tttcttttcg
     3121 aattcgctgg agatttccat caaagaatct tcaggttttc aaaattattt tggaatttta
     3181 ggatcccaaa ataattctat tgagagtttg atcctggctc aggatgaacg ctggcggcgt
     3241 ggataaggca tgcaagtcgc gtgggtttag gcccacggca aacgggttag taatgcatag
     3301 gaacgtaccc tcgagtctcg aataatcctt cgaaaggagg aataatgcga gatagttctc
     3361 ttcggagtaa agatttatcg cttgaggagc ggcctgtgtc ctatcagctt gttggtgagg
     3421 taaaggctca ccaaggctat gacgggtagg ggatgtgaga gcatgatccc caacattgga
     3481 actgagacac tgtccaaaca cctacgggtg gctgcagtcg agaatcttcg gcaatgggcg
     3541 aaagcctgac cgagcgacgc cgcgtgaagg atgaaggcct tcgggttgta aacttctttt
     3601 gcaagggaag aaactatgca caattgagaa attcattttt taattatgga ttttttaact
     3661 gtgcatggct gacggtacct tgcgaataag gggttgctaa ctctgtgcca gcagcagcgg
     3721 taatacagag accccaagcg ttatccggat ttattgggcg taaagcgtgt gtaggcggca
     3781 atgatagtca gatgttaaat tccagcgctc aacgttggag ctgcatctga aacgtcatag
     3841 ctagagacaa gtagaggttc atggaacata tggtgtagca gtgaaatgcg ttgatatcat
     3901 atggaacgcc aaaggcgaag gcagtgaact gggctttgtc tgacgctgag acacgaaagc
     3961 gtgggtagcg aacgggatta gataccccgg tagtccacgc cgtaaacgtt gcctactagg
     4021 tctggggagt atcgaccctc tccggaccga agctaacgcg ttaagtaggc cgcctgggaa
     4081 gtacgagcgc aagcttaaaa ctcaaaggaa tagacgggga cccgcacaag cggtggagca
     4141 cgcggttcaa ttcgtctcta agcgaggaac ctcaccaggg cttgaaatgc agctgcaaat
     4201 cctaggaaac taggacttct tcgagagtgc tgcgtaggtg ttgcatggtt gtcgtcagct
     4261 cgtgccgtga ggcgttccat taagttggga aacgagcgca acccctgtcg tgtgttacat
     4321 gtgtcacacg agactgccca gcccagagtt catgaaatat attcgtgaaa tccgggctgg
     4381 gaggaaggtg gagatgacgt cagatcagca tggcccttac gtcctgggcg acacgcatgc
     4441 tacaatggcc ggtacaatgg gtagccaacc cgcgaggggg agccaatcca aaaaagccgg
     4501 tcccagttcg gattggagtc tgcaactcga ctccatgaag ccggaatcgc tagtaatcgt
     4561 gaatcagcca tgtcacggtg aatacgttct cgggtattgt actcgggtgc cttgcagcct
     4621 gataaattag tcggtgaaag tccggccagg gatattgaag aatccatgaa gcgaaatcgc
     4681 aggttcgact ccgtgaggat cggactgaag ggcgaaagta gcagatacca gcttgaacga
     4741 acaataaatg catatatatt tggcgactct gcgagtacat gaggaagcta tcttcaaata
     4801 taaatatata cgcatcggtt cgtcgagacg gcgtcaaaat gtttattagg tgaccaaggg
     4861 agatcttgta tggtctttta attctgtggt atactaacca cgagaattat agggtaagat
     4921 taatgggtaa tcattgattc aagccatgca agaagtcagc cgagctcata gtaccaaacg
     4981 aaaacctttc gagcgcaatc cgcgactgaa agatccgttt gggaaggccc tcctcccc
//
LOCUS       DATKGS010000037         5473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_160963, whole genome shotgun sequence.
ACCESSION   DATKGS010000037 DATKGS010000000
VERSION     DATKGS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160963"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VMQ48_00895"
     CDS             complement(<1..538)
                     /locus_tag="VMQ48_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08627.1"
                     /translation="MRIGINASFLRKTDTGMGQVSRGFISELVKSYSDKNEYFLYLEK
                     DIDFKLPKNFHKRIFLPAWKRDDLIRKIWWEKFLLPKYVKRDQCDMFISLYQCPTFLP
                     KNIRHKMLVHDLIPKIFPDYLDNSRKKFYFFLTQKAIQHADEVITVSEWSKKDIHKFL
                     NIPLGKIKTAYPSIGEEFF"
     gene            628..1581
                     /locus_tag="VMQ48_00900"
     CDS             628..1581
                     /locus_tag="VMQ48_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD08628.1"
                     /translation="MRLIVNIPAYNEEKTIAGVIHKIPRQIHGIDEVKIQVIDDGCTD
                     KTVEVAKAAGADFVVSHTRNMGIGAAFKTAVESALENGADIMVNIDADGQFPPSDIPK
                     FTELIVSHKADMVVASRFGIKSAKNMPWIKSFLNRFAAKVIGKFLRHPIDDLTCGFRA
                     LSRETMLRLNLAYRFTYTQETIVDAISKNLVVKWIPVEVHYFDDRQSKVVKSVGKFVY
                     QSFLIIIRTVRDARPMVFFGIPGVTLITTAAAIGIAFLGYYFSHFRVSPFRTWLSLAG
                     ILLLIGLQLLVFALIADMIRNNRKLTEEQMYFLKKEKYNKK"
     gene            1578..2693
                     /locus_tag="VMQ48_00905"
     CDS             1578..2693
                     /locus_tag="VMQ48_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUD08629.1"
                     /translation="MKILFVSHSYPPVWGGVESQNYNLAENLKKIAEVKVVANGKGKK
                     YLPVFLPVTLFRMLFLMRNCDVCLLGNGVLAPWGAIAKIFHPRKKFFCVVHGLDITFA
                     NKKGFLSKVYKNINIPSLKKLDRLLMVGNATIEEAVKIGIKREQCVFIPNGIDPKEFS
                     EKHEREEISKILGTDINDKKIIFRLGRFVPHKGTSWFIENVMPKLSENVILVAAGGRV
                     GKNTAGDKDDFAVCEKVVKKKNLGSRVWLFPNIPEQQKKTLLNAADLVVSPNIKVPGT
                     MEGFGINVVEAAVCGRMVAASALEGLKDAVKENENGFLVECGNADAWARKIKELLADD
                     EFRKKFGEKAREYTVDNFSWDKIAKRYLEEMEKVAKK"
     gene            2700..4622
                     /locus_tag="VMQ48_00910"
     CDS             2700..4622
                     /locus_tag="VMQ48_00910"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HUD08630.1"
                     /translation="MVFSLLKLILTIPFVLVLPGVFFQLAIFGWKSAFGGGKISFFEK
                     AVLAVPFSLIIVDLIVLLLNKMNILLKGPVLVGAILIFCLVCFAFFQFRFGNRLLVDP
                     PERRRTEEGDKQSLFNFSTWQTIFILLAIVLAIFIRTAYLSDTVIPSATDLGHHMYWV
                     QTIINTGHLPDYGVPDFIIGEHMIFAVVNLISGVKITTAMPVLLLLLFNITGIFTIAI
                     LLGRVFENNKIVAMSFFVAGSLYAINPPQGRYVSGGVVGNVIGDMLIPVAMYFFYRVL
                     KERDQIFAGLFIFSFAGLLYTHHLSAFVLIFSIAAVVILYLILNFRNGFKIISEWTKL
                     FLKPFPLALIFIFALYFLLIFTPSYFNPAAVGQATGEPTKNTRIGFGIAQIDSGVGSA
                     LLVLGAAGFLILMLELFKEKILSARGGSAFGGKHAFAIGWFLVLFTMTWKPGLLYVNI
                     PSDRVSNYLFLPLVFLSSYALVRYFEVFTSATSRFFSTIFLFMLLFFVITNGLSDSAD
                     VFKTKNQFQGIMETFHSAAYLAATVDTNKDIILKDHVNIYGDSWYKLFFMKDYKYPLS
                     RGVLMRYVDPTKPRETCTRDMISDPESDSGKACFVDTGVNYVVVNAQIEGNSFEKYPD
                     FSKVYGSDYISIFRRD"
     gene            4744..5109
                     /locus_tag="VMQ48_00915"
     CDS             4744..5109
                     /locus_tag="VMQ48_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08631.1"
                     /translation="MSSKIIIIAVAILLIASFSVLFVIEAKNHNYDYNKAWSVAYFEN
                     PRDNSLDFAIENHEGVAAEYGYKIFVGEDKVIDEKVEINAGATQKISPVIPSDKLKGN
                     KVLIDVNFKGTDYKIYKSL"
     gene            5110..>5473
                     /locus_tag="VMQ48_00920"
     CDS             5110..>5473
                     /locus_tag="VMQ48_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08632.1"
                     /translation="MKRILLLTRPICPPWDEGSKNFAYTLAKNAGDFEIHLLTCGKVA
                     NLPKNVIQHEIYSSPKLDFGQKFRLLKFLKKANDFDIIHSLFTPTKINSFFLRLNLKK
                     GIKTIQTVATLREDLYSAE"
BASE COUNT         1487 a         1107 c         1268 g         1611 t
ORIGIN      
        1 caaaaaactc ttctccaatg ctcggataag ccgtctttat cttgccgagc gggatattca
       61 agaacttgtg gatatctttt tttgaccact cggaaacagt gatgacctca tccgcgtgtt
      121 ggattgcttt ttgtgtcagg aaaaaataga atttttttcg tgaattgtcg agatagtccg
      181 ggaatatttt cgggatcagg tcgtggacaa gcatcttatg ccggatgttt ttcggcaaaa
      241 aggtcggaca ttgatagagg gaaatgaaca tatcgcactg atctcgtttg acatatttgg
      301 gtaaaagaaa tttttcccac cagatttttc ggataaggtc atctcgtttc caggccggca
      361 gaaatatcct cttgtggaaa tttttcggca gcttgaaatc aatatctttt tcgagataca
      421 aaaaatattc atttttatct gaataagatt tgaccagttc gctgatgaat ccgcgagaaa
      481 cttggcccat tcccgtatcc gtctttcgca aaaaagaagc gtttatcccg attcgcatat
      541 ttgaattcta caacaaatgt ttgattaaaa caaattattt tgctagtata tatccagtgt
      601 ttaaccctaa ataatatagc tatttctatg cgtttaattg tcaacatccc ggcgtataat
      661 gaagaaaaaa caatcgccgg agtgatccac aaaattcccc gccaaatcca tgggatcgat
      721 gaagtgaaaa ttcaggttat tgatgatggc tgcaccgaca aaacggtcga agtcgccaaa
      781 gcggctgggg cggatttcgt cgtgagtcat acgcgcaata tgggcattgg cgcagctttc
      841 aaaacggctg ttgaatcggc tcttgagaat ggagcggata ttatggtgaa tattgatgct
      901 gacggccaat ttccgccaag cgatattcca aaattcacag agctgatagt tagtcataag
      961 gcagacatgg tggtagcttc ccgctttggg atcaaatcgg ccaagaatat gccctggatc
     1021 aaaagttttc tcaatcgctt tgcggcaaag gttatcggga aatttttgcg tcatccgatt
     1081 gacgacctca cctgcggttt tcgagcgctt tcccgcgaaa cgatgctgcg actcaatttg
     1141 gcctatcgct tcacatatac gcaggaaacg attgttgatg ccatttcaaa aaacttggtt
     1201 gtgaaatgga tcccggtgga agttcactat ttcgacgatc gtcagtcaaa agtcgtgaaa
     1261 agtgtcggga aatttgtcta tcaatcgttt ctaatcatca tccgcactgt tcgcgacgcg
     1321 cgtccgatgg tattttttgg gattcctggg gttacgctta tcacgacggc tgctgccatc
     1381 ggaatcgcct ttttgggata ttatttctct catttcagag tttcaccttt tcgtacttgg
     1441 ctctcgctgg ccggaattct gcttcttatt ggtctgcagc ttctggtatt tgctttgatt
     1501 gccgatatga tccgcaacaa ccggaagctc accgaagaac agatgtattt tttgaaaaaa
     1561 gagaaataca ataaaaaatg aaaattcttt ttgtttccca ttcctatcct ccggtttggg
     1621 gcggagttga aagccaaaac tacaaccttg ctgaaaatct caaaaaaatt gctgaggtga
     1681 aagttgttgc caatgggaag ggtaaaaaat acttgcctgt ttttttgccg gtgacgcttt
     1741 tcaggatgct cttcctgatg cgcaactgcg atgtttgtct tctgggaaac ggtgttttgg
     1801 caccctgggg agcaatcgcg aaaatattcc atccgcgaaa gaaattcttt tgcgtcgtgc
     1861 acggtttgga tatcacattt gccaacaaaa aaggcttttt gtcgaaggta tacaaaaata
     1921 taaatattcc ttccctcaaa aagctggatc gtttgctgat ggttgggaat gcgacaattg
     1981 aggaagcagt gaaaatcggc ataaagcggg agcaatgcgt atttataccg aacggaattg
     2041 atccgaaaga attttccgaa aagcacgaac gtgaggaaat ttcaaaaatt ttaggcactg
     2101 atataaatga caaaaaaatt atttttcggc tggggcgatt tgttccgcac aaaggaacga
     2161 gttggttcat tgagaacgtg atgccaaaat tgtcggaaaa tgtcatactg gtggcagccg
     2221 gtggaagggt cggaaaaaat actgccggcg acaaagatga ttttgctgtt tgcgagaaag
     2281 tggtcaagaa aaaaaatctc ggtagccgtg tttggctttt tccgaatatt cctgagcagc
     2341 agaaaaaaac attgctgaat gcagctgatc tggtcgtctc cccaaacata aaagttccgg
     2401 gaacaatgga aggcttcggg atcaatgttg tggaggcggc ggtctgcgga agaatggttg
     2461 cagcctcggc gctcgaaggc ctcaaggatg ctgtgaaaga aaacgaaaac ggatttttgg
     2521 ttgaatgcgg aaatgccgac gcctgggcgc ggaaaatcaa ggaacttttg gcggatgacg
     2581 aattccgaaa aaaatttgga gaaaaagcga gagagtatac ggtcgataat ttcagttggg
     2641 ataaaatagc gaaaagatat ctggaggaaa tggaaaaagt cgcgaaaaaa tagaaattta
     2701 tggtttttag tcttctaaaa ctcatcctca ctatcccctt tgtcctcgtc ttgccgggcg
     2761 tgttttttca gttggcaatt ttcggttgga aatccgcctt tggcggagga aaaatatctt
     2821 ttttcgaaaa agcggttttg gctgtgcctt tcagcttgat catagttgac ctgatcgtgc
     2881 tgcttctcaa caaaatgaat attttgctca aggggccggt gctggtcggg gcgattttga
     2941 tattttgtct cgtatgtttt gccttttttc aattcagatt tgggaatcgc ctcctcgttg
     3001 atccgccgga aaggcggaga acggaagaag gagacaagca aagcctattt aatttttcca
     3061 cctggcagac tatattcatc ttgctggcaa ttgtacttgc cattttcatc cgcacagcct
     3121 atctctctga cacggtcatc ccttctgcga ccgatctggg acaccatatg tattgggtcc
     3181 agacgatcat caacaccggg catctgcccg actatggcgt gcctgacttc ataatcggag
     3241 agcatatgat ctttgcagtt gtgaatttga tcagcggggt gaaaattaca acggctatgc
     3301 cggtgctttt gctgctgctt ttcaacatca ccgggatttt cacgattgcg atccttctcg
     3361 gccgggtttt tgagaacaat aaaattgtag ccatgtcatt ttttgtagcc ggttctttgt
     3421 atgcgatcaa cccaccccag ggtcgatatg tgagcggcgg agtggtggga aatgtgatcg
     3481 gcgacatgct tatcccggtc gcaatgtatt ttttttatcg cgtcctcaag gagagggatc
     3541 aaatcttcgc cggattgttc attttttctt ttgcaggcct tctatacacg catcatttga
     3601 gcgcttttgt gctgattttc tcaattgctg cggttgtgat tttatattta attctcaatt
     3661 tcagaaacgg cttcaaaatt atcagtgaat ggacaaaatt attcctaaaa ccttttccac
     3721 ttgcgcttat atttattttt gcactgtatt ttctcttaat tttcactcct tcatacttca
     3781 atcctgccgc tgtcggtcaa gcaacgggcg aaccgacaaa gaacacgcgg atcggatttg
     3841 gtatcgccca gattgattca ggcgtcggca gcgcgctgct tgttttggga gcggcggggt
     3901 tccttatatt aatgttagag ttgtttaagg aaaaaatatt gtccgcccga ggcggatccg
     3961 cctttggcgg aaagcatgcg ttcgcaatcg gttggttttt ggttttgttc acaatgactt
     4021 ggaaaccggg attgctctat gtcaacattc cgagcgaccg ggtgagcaat tatttgttcc
     4081 tgcctttggt attcctctct tcttatgcac tcgtccggta ttttgaggtt ttcacctcgg
     4141 caacatcgcg ctttttttcg actatttttc tgtttatgct tttgtttttc gtcattacga
     4201 atggtctttc agacagtgcg gatgttttca aaaccaaaaa tcaattccaa ggaattatgg
     4261 agacttttca ctcggccgct tatttggccg caacggtgga tacaaacaag gacattatct
     4321 tgaaagatca cgtgaacata tacggcgatt cttggtataa attgtttttt atgaaagact
     4381 acaaatatcc cctctcccga ggggtgctta tgcgatatgt cgatccaacg aaaccgcgcg
     4441 agacttgcac gcgggacatg atctcggatc cggaaagcga ctcgggaaag gcttgttttg
     4501 tcgacacggg agtcaattat gtcgtcgtaa acgcccagat tgaaggcaat agttttgaaa
     4561 aatatccgga cttttcaaaa gtctatggtt cggattatat atcgattttt agaagagatt
     4621 agaatactaa cctcctcccc tgccaagggg aggtgcccga agggcggagg ggttttaaat
     4681 tccgcaaagc aaaccacccc tcaatcccct ccttgaaaaa ggaggggagg ttaagaaaaa
     4741 gatatgtcat ccaaaatcat cattatcgcc gtcgcaattc tcctcatcgc cagcttctcg
     4801 gtgctttttg tcattgaagc caaaaatcac aattatgatt acaataaagc ctggagtgtg
     4861 gcgtatttcg aaaacccgcg ggacaactcg cttgatttcg cgattgaaaa tcatgaaggt
     4921 gtggcggcgg aatacggcta caagattttt gtgggcgaag acaaagtgat tgatgaaaaa
     4981 gtcgagatca atgccggcgc aacgcaaaag atctcgccgg tgattccgag cgataaattg
     5041 aaaggcaata aagttttgat cgatgtgaac ttcaagggga cggattataa gatatataag
     5101 agtttataaa tgaaacgcat attattattg actcgtccga tttgtcctcc gtgggacgaa
     5161 gggtcgaaaa atttcgccta tacgcttgcc aaaaacgcag gcgattttga aattcatctt
     5221 ttgacgtgcg ggaaagtagc aaatcttccg aaaaatgtga ttcagcatga gatctattca
     5281 tcgccgaaac tcgatttcgg gcagaaattc aggctgctca aattccttaa aaaagctaat
     5341 gattttgaca ttattcattc gctttttact ccgacaaaaa taaattcctt ttttttaagg
     5401 ctaaacctta aaaaaggaat aaagactatt caaactgttg ccactttacg cgaagatttg
     5461 tatagcgcgg aag
//
LOCUS       DATKGS010000038         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_161230, whole genome shotgun sequence.
ACCESSION   DATKGS010000038 DATKGS010000000
VERSION     DATKGS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161230"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VMQ48_00925"
     CDS             complement(<1..1761)
                     /locus_tag="VMQ48_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HUD08633.1"
                     /translation="MDLNKLTFKSQEALQGAQQVSQNLQQNMVDVLHLLYTLLGQSDS
                     VVPTIVDKLEADREELKKKIYTEIEKIPKVTGAPQFYLSPEMAGVLQEAFKEAEAMGD
                     EYISTEHLFAALLKIASRAKKILEENSINSEKVSKIMKEVRGNQRVDSPTPEGKYQVL
                     EKYTINLTKEAAAEKLDPVIGREEEIRRIMQVLTRRTKNNPVLIGEAGTGKTAIAEGL
                     AQRIVAGDVPETLKGKEILALDLGSLLAGSKFRGEFEERFKAIMNEIEKSAGKYILFI
                     DELHTLVGAGAIEGSMDASNMIKPALARGKVRAIGATTLKEYQRYIERDAALERRFQP
                     VYVSEPSIEDTVAILRGLKEKYEIHHGVRITDGALIAAAELSNRYITDRNLPDKAVDL
                     IDEATSSLRMEIDSMPVELDQFEREKRKLEIEKEALKKEKDKQSKARLKEIEKKLADI
                     NEKASKQTLQWKSEKDLITNIRTHKSEIERLRMESDVLERQGDLARVAEIRYGKIPEF
                     ERKIKNEQRKLTQMQSKNPILKEEVTEEDIAGVVSRWTGVPVSKMLEGEMEKLVAAEE
                     SLKTRVVGQEEAISAVANALR"
     gene            complement(1940..>2426)
                     /locus_tag="VMQ48_00930"
     CDS             complement(1940..>2426)
                     /locus_tag="VMQ48_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HUD08634.1"
                     /translation="GAIGHGATISQPLTVAFMLEKLQPRLGDKVLDIGAGSGWTTALL
                     AHIVGKKGKVIGIERIKSLCDFGTNNISKYNLIEGGRAKIICADGSKGYVNEAPYQRI
                     LVSAAAEEIPKELKKQLVVGGRLAIPVKDSIWMIEKKSDKIFHEEEYPGFAFVPLVSK
                     G"
BASE COUNT          484 a          691 c          522 g          729 t
ORIGIN      
        1 gcgcaatgcg ttcgctaccg cgctgatcgc ctcttcctgg ccgacgaccc gcgttttgag
       61 gctctcctcg gcggccacca atttttccat ctcgccttcg agcatcttgg aaacaggtac
      121 gcccgtccag cgggaaacca ctcccgcaat atcttcttca gtcacctctt ctttgaggat
      181 cggatttttg ctctgcatct gggtgagttt tctttgctcg tttttgattt tccgctcaaa
      241 ctcgggaatt tttccatacc ggatttccgc cacgcgcgcc aggtctccct gccgctcaag
      301 cacgtccgat tccattcgca gccgctcaat ctcgcttttg tgcgtgcgga tattggtgat
      361 caaatccttc tcgcttttcc actgcaaagt ctgcttgctg gctttttcgt tgatgtcggc
      421 caattttttt tcgatttctt tgagccgcgc cttggactgc ttgtcttttt cttttttgag
      481 cgcctctttt tcgatttcca gttttctttt ttcccgttcg aactggtcaa gctcgaccgg
      541 catgctgtcg atctccattc gaagggatga agtcgcttca tcgatgagat caacggcttt
      601 gtcaggcaag ttccgatcgg taatataccg gttggaaagt tcggccgccg caataagcgc
      661 accgtccgtg atccgcacgc cgtgatggat ttcatatttt tccttgaggc cacgcaggat
      721 cgccaccgta tcttcgattg acggttcgct gacatacacc ggctgaaatc ggcgttcaag
      781 cgccgcgtcc cgctcaatgt atctttgata ttctttgagg gtggttgctc cgatcgcccg
      841 cacttttccg cgcgcgagag ccggcttgat catgttcgac gcgtccattg atccttcgat
      901 cgcgcccgcc ccgaccaatg tgtgcagctc atcgataaac aaaatatatt ttccggccga
      961 tttttcgatt tcattcatga tcgccttgaa ccgctcttcg aactcgccgc gaaacttcga
     1021 tcccgccaaa agcgagccga gatcaagcgc taaaatttct ttgcccttga gcgtttccgg
     1081 aacatcgccc gcgacaattc tttgcgccag cccttcggca atcgccgttt ttcccgtccc
     1141 tgcttcacca atcagcaccg gattattttt ggtccgacga gtgagaacct gcatgatgcg
     1201 tcggatttcc tcttcacgtc caatgaccgg atcaagtttt tccgccgccg cttctttcgt
     1261 cagattgatc gtatattttt cgagcacctg atattttccc tcaggcgtcg gactgtccac
     1321 tcgctgattg cctcgcactt ctttcataat ttttgaaact ttttcgctgt taatgctgtt
     1381 ttcttcgagt attttttttg cccggctcgc aattttgaga agagccgcaa agagatgctc
     1441 cgtcgagatg tattcgtccc ccatcgcctc ggcttctttg aaagcttcct ggagaacgcc
     1501 cgccatttcc ggagaaagat agaattgcgg cgcgcccgtc actttgggaa tcttttcgat
     1561 ttcggtatat attttctttt ttagctcttc ccgatccgct tcaagcttgt cgacgatggt
     1621 cggcacgacg ctgtccgact gcccgagaag agtatagagc aaatgcaaaa catccaccat
     1681 attctgctgg agattttgcg aaacctgctg cgccccctgg agtgcctcct gcgacttgaa
     1741 agtgagttta ttgagatcca taatttgttg ttattatcgt tagcactctc atcaatcgag
     1801 tgctaattta ttatacctcc tcccgcacct ctgtcaagga ttattacgca gtctacccca
     1861 tatatatttt atcttataaa caaaaagctc tcttcgtcaa aagaagaaag ctgtatggct
     1921 tctaatggaa aaaattattt caaccttttg aaaccagcgg cacgaaagca aatcccggat
     1981 attcttcttc gtggaaaatt ttgtccgatt ttttttcgat catccagata ctatccttca
     2041 ccgggatagc caagcgcccc ccgacgacaa gctgtttttt gagttctttg gggatttctt
     2101 cggccgcagc ggagaccaaa atgcgctgat aaggcgcttc gttgacatag cccttggagc
     2161 cgtccgcgca tattattttc gcccggccgc cttcgatcaa attatatttg ctgatattgt
     2221 tcgtcccgaa atcacaaagg ctcttgatcc gttctatgcc gatcactttt ccctttttcc
     2281 cgacgatatg cgcgagaagc gccgtcgtcc aacccgatcc cgcgccgatg tccaaaacct
     2341 tgtccccaag gcgaggctgc aatttttcca acataaaagc caccgtcagt ggctgagaaa
     2401 tcgttgcgcc gtgaccgatc gccccc
//
LOCUS       DATKGS010000039         7345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_16503, whole genome shotgun sequence.
ACCESSION   DATKGS010000039 DATKGS010000000
VERSION     DATKGS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16503"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /gene="fmt"
                     /locus_tag="VMQ48_00935"
     CDS             complement(<1..673)
                     /gene="fmt"
                     /locus_tag="VMQ48_00935"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HUD08635.1"
                     /translation="MSTKKNLKIIFFGTPKFAAVILKSLLDNKFDMAAVFTQPDKKVG
                     RKQEVAYSPVKDLAQKNKIEIFQPQSLRESGLSEAIKEIKPDLFVVAAYGKILPKDIL
                     EIPELGAVNIHTSLLPKYRGASPVQCAILSGAKKTGITLMKMNEKMDEGDILVQEKIK
                     IGENDTTDTLMKKMAELGAKMITNFIPGWVEGKIKPVRQDHSKATYCKPVKRDDGKID
                     WNSPAE"
     gene            complement(680..1129)
                     /gene="def"
                     /locus_tag="VMQ48_00940"
     CDS             complement(680..1129)
                     /gene="def"
                     /locus_tag="VMQ48_00940"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUD08636.1"
                     /translation="MLKIIKYPNDFLRKKTREVKVKELKDPKIQRFILDMAKTMEVEK
                     GVGLAAPQVGSDLRICTIQADDEAYVLVNPKIKSSSRKKDIFEEGCLSFPGKFFPIER
                     PIKVKVQARDREGKKVKIKADGLFARVLQHEIDHLDGILVIDRAIKE"
     gene            complement(1134..3080)
                     /gene="priA"
                     /locus_tag="VMQ48_00945"
     CDS             complement(1134..3080)
                     /gene="priA"
                     /locus_tag="VMQ48_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HUD08637.1"
                     /translation="MKKYIIQVAPLVPLPVLRTQVFSYWHDEPLPIGTLVSIPFYFRE
                     IEGIVVKVRGDISQTGSFKLKNIRAVKEKNFLNENQIDLARRIAEYYFSPLGSVLKLM
                     IPKIVQRREKNKKISKKTIQMGKAGEIALRILKSDKKKFFLAGNAKQREKTNVDLIRA
                     CLKRNKQCLILAPEIFFSQSIFDKLQGVFSEEIALIHSKITKGQYCEFWKKIKDGQIK
                     IVIASKMGIFLPFLDLGLIVVQEDQDPSFKQWKAMPRYNGARGAEFLAQISGAKLVLE
                     SFAPSVENLYQSRENDLEILSLNSNLPKIEITFLDKVASSATRRGGGNADFPISGGLY
                     SRLAEIVKSKKQAVVFMNRRGFSTRTICENCKKPLKCPKCDLALVFSDEHSEYHCLHC
                     TFKMDLLSSCPTCGGFQFIHRGIGTQTVEKKIKRLFPSARVVRLDADVMKSQAKYKSI
                     LREFLAGEIDILVGTQSAIKGIYSENIKLAASISGKDFADEINFNSRALALSRVFNMA
                     NLLSEDGVLLIQSFFGGSPLFDFLEKKELKKYFTQELAMRKRLGYPPFRKLVKLSFRD
                     KSEKRAASETKKAFDLLRATGNNTVEIIGPYIPFSGEKKGIYTRNILIKCDPEKNISD
                     LPIRAVIGGLGKGWSVDVDPISIM"
     gene            complement(3077..3874)
                     /locus_tag="VMQ48_00950"
     CDS             complement(3077..3874)
                     /locus_tag="VMQ48_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HUD08638.1"
                     /translation="MIRFSAITPHPTVIVPSIGKSNLNLVRKTANAMKQIGEKFRAVK
                     ADTIVLISPHGPVQYDKFMINYSPTLRGDLGSFGDQRRFVFKNNRELVDEIMKLARES
                     RLPVGLVRELELDRGALVPLHYLVSEKDTAYNPKLIHMSFSMLPLEYHFNFGRVLGKV
                     LQESPRVISIIASSDLSHRVSPGSPAGYSPQGRIFDKKLVDLLKRKDIKGILEIEPEL
                     IEEAGECGLRSIVMMLGAISNLRWDIRSMSYEAPMGVGYLAANINIK"
     gene            3973..5301
                     /gene="glyA"
                     /locus_tag="VMQ48_00955"
     CDS             3973..5301
                     /gene="glyA"
                     /locus_tag="VMQ48_00955"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUD08639.1"
                     /translation="MYKNLKKQDPAIYKAILAETKRQEEGLELIPSEIAASAAVIEAL
                     GSVLTNKYSEGYPRKRYYGGQENIDTIEEIAQERAKKLFGVPHANVQPYSGSPANLAV
                     YVAVCNPGDVVLGQNLPDGGHLTHGWKASATSMFYKTIGYHVNEDGYLDLEEIERLAK
                     KNKPKLIWVGATAYVREFPFEKMAKIADKVGAYLAADIAHIAGLVISGAHKSPAKFAH
                     IITTTTHKTLRGPRGGMIMVTHKGLKKDPDLAKKIDQAIFPRLQGGPHDHQTAAIAVA
                     LGEAMKPSFKTYGHQVVKNAKALAKALMARGVKIVTNGTDNHMILIKCGKGRGVFLQD
                     ALDAAGITLNKNTIPKDPSSPFYPSGVRLGTPFITVRGMKEKEMEKLADWIAQVMEEI
                     KDFQFPSDKAKIKPTLIKFRKFVAGNKELKKIKSEVKKLCAKFPLYKDLK"
     gene            5369..6205
                     /locus_tag="VMQ48_00960"
     CDS             5369..6205
                     /locus_tag="VMQ48_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HUD08640.1"
                     /translation="MKLLNGTKLANKIILQLKNKVAIMTRKPVLAMVLVGNDPASKIY
                     VGRKKILCEKAGIGSQIFTFPEKTSEKKILEIIDGLNKSQNISAIMVQLPLPKHIDKD
                     KIIEAIDPRKDADCLHPLNFGKFTQEGEKYSVVAPATPLGIIKLLEEYKVKIEGKNAV
                     IVGRSNIVGKPMAQMLLNRNATVTICHRHTKNLKSYTQKADILVVATGKMNMITADMV
                     QKGVVVIDVGVNRGENKKVFGDVDFKSVSKKASFITPVPGGVGPMTIAMLLWNTVKLT
                     EK"
     gene            complement(6244..>7345)
                     /locus_tag="VMQ48_00965"
     CDS             complement(6244..>7345)
                     /locus_tag="VMQ48_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HUD08641.1"
                     /translation="FYFSYYTGIQKFKIQAFLKTNRGILRIVFIIGLAFLLKSHGMAL
                     QGAILGYVVAPLSIFFEAWSLDPYKKLKSVGTFDWKKLVVFAWPVTIFLIAYQLLNTI
                     DLYLVKGILRSDFQTGIYNAAITVGTIPYNLFYALTIILLPSISKSTAANNFDETKKI
                     ISQSLRFLVMFLVPVCLLMSYFSLPIIQIFFSSKYALSAPVMSVYVIAEGFLTVFYVL
                     TFILNGAGKVKIPMYVAILGLFLNTFLTYFLIKSYGLLGAAGGTAFMALAVMIFGLVY
                     THRSFGYLFKASSFFKIILASAAMSALAFVFPRNNYYFILWSVLLFGIYLIILYILRE
                     IGAGDLVLVKELLSRKKSKRETVEDTEFIDGK"
BASE COUNT         2099 a         1753 c         1592 g         1901 t
ORIGIN      
        1 cttcagcagg gctgttccaa tcaatttttc cgtcatctct tttcaccggc ttgcaatatg
       61 tcgccttgct atgatcttgc cgaaccggct tgatttttcc ctcaacccaa ccgggaatga
      121 agttcgtaat catctttgcc cccaattcgg ccattttttt catcaacgtg tcggtggtgt
      181 cattttcacc tatttttatt ttttcctgaa ccaaaatatc accctcgtcc attttttcgt
      241 tcattttcat caaggtaatg cccgtttttt ttgctccaga gaggatagcg cattgcaccg
      301 gcgatgcgcc acggtatttc gggagcaggg aagtgtgtat attcaccgcg ccaagttctg
      361 gaatttccaa gatatctttt ggcaaaattt tcccataagc agcgacaaca aaaagatccg
      421 gctttatttc ttttatcgct tcgctcagtc cgctttcgcg caggctttgc ggctggaata
      481 tttcaatttt atttttctgc gccaggtctt tcaccggaga atatgcgact tcttgttttc
      541 ggccaacttt cttgtccggc tgagtgaaaa cagccgccat atcgaattta ttgtcgagaa
      601 gagatttcaa aatcaccgcc gcaaatttgg gagtaccaaa aaaaataatt tttagatttt
      661 ttttcgtaga cattttttgc tattccttta ttgctcggtc gattaccaaa atcccgtcca
      721 aatggtcgat ttcatgctga agcacccgcg caaagagacc atccgccttg atcttcactt
      781 ttttcccctc tctgtcccgc gcctgcactt tcactttgat cggccgttcg atcggaaaaa
      841 attttcccgg aaaactcaag cagccttctt caaaaatatc ctttttccgc gaagatgatt
      901 ttattttcgg attgaccaag acatatgcct cgtcatcagc ctggatcgtg cagatccgca
      961 gatctgatcc gacttgcggc gcggccaagc caactccctt ctcgacttcc atcgttttgg
     1021 ccatatccaa aataaaccgc tggattttcg ggtctttcaa ttctttgact ttgacttccc
     1081 gcgttttttt tcgcaagaaa tcatttggat atttgattat tttgagcata cttttacatt
     1141 attgaaatcg gatccacatc cacactccag cccttgccga gccctccaat gacggcacgg
     1201 atcggcaggt cgctgatatt tttttccgga tcgcacttta tgagaatatt tcgcgtatat
     1261 atgccttttt tctctcccga aaacggaatg tatggcccga ttatttcaac cgtattattg
     1321 cctgtcgcgc gcagcaagtc aaatgctttt tttgtttcgc ttgcagccct tttttcagat
     1381 ttgtcgcgaa aagaaagttt tacaagtttt cgaaacgggg gatagcccaa tcttttccgc
     1441 atcgcgagct cttgagtgaa atattttttc aattctttct tctccaaaaa atcaaaaagg
     1501 ggactgccgc cgaagaaaga ttggatcaac agaactccgt cctcgctcag caaatttgcc
     1561 atgttgaaaa cccgggaaag cgcgagagct cttgaattga aattgatctc gtccgcgaag
     1621 tcttttccgg agatcgaagc agccagtttg atgttctccg aatatattcc tttgatcgcc
     1681 gactgcgtcc cgacaagaat atcgatttca cccgccaaaa attcccgcag gatgctcttg
     1741 tatttcgcct ggctcttcat cacatccgcg tccagccgga cgactcgcgc cgacgggaaa
     1801 agtcgtttga tttttttctc aaccgtttgc gtcccgattc cgcgatgaat gaattgaaat
     1861 ccgccgcacg tcggacagga actcaaaaga tccatcttga aggtgcagtg caaacaatga
     1921 tactcactat gttcatcgga aaaaacaagc gccaaatcac atttcggaca tttcagcggt
     1981 tttttgcaat tctcgcaaat cgtgcgagtg gaaaaaccgc gcctgttcat gaaaacaacg
     2041 gcctgttttt tgcttttcac gatctcggcc aaacggctgt ataatccccc agagataggg
     2101 aagtcggcat tcccgcctcc tcggcgggtc gccgatgagg cgactttgtc cagaaaagta
     2161 atctcaattt ttggaagatt actgttcaaa ctcaatatct ccaagtcatt ctctctcgat
     2221 tgatacaagt tttccaccga cggcgcaaaa ctttccaaaa ccagtttcgc gcctgaaatt
     2281 tgcgccaaaa attccgctcc gcgcgcgccg ttgtagcgcg gcatggcttt ccattgcttg
     2341 aaagaaggat cctggtcttc ctgaacaaca atcaagccta aatcgagaaa cggcaaaaat
     2401 attcccattt tcgaggcaat cactattttt atttggccgt ctttgatttt tttccaaaat
     2461 tcgcagtatt gcccttttgt tatcttgctg tggataagcg cgatttcctc ggaaaaaact
     2521 ccctgcaatt tgtcgaaaat gctttgcgaa aaaaatatct ccggcgcgag aatgaggcat
     2581 tgcttgtttc tcttgaggca agctcgaatc aaatcgacgt ttgttttttc ccgctgcttg
     2641 gcgtttccgg ccaggaaaaa cttcttcttg tcgctcttga gtattctcaa ggcaatctct
     2701 ccagcttttc ccatctgtat cgtttttttt gatatttttt tgttcttttc tctccgctgc
     2761 acaattttcg gaatcatcag cttgagaact gacccaagcg gagaaaaata gtattcggcg
     2821 atccttcgcg caagatcgat ctgattttca ttcaaaaaat tcttctcttt gaccgccctg
     2881 atgtttttca atttgaaaga acctgtttgc gaaatgtccc cgcgaacttt gacgacaatc
     2941 ccttcaatct cgcgaaaata aaaagggatc gaaaccagcg tcccgatcgg caagggctcg
     3001 tcgtgccaat aagaaaaaac ctgcgtgcgc agaaccggaa ggggaacgag aggagccact
     3061 tgaatgatat attttttcat ttgatattga tattcgctgc gagatatccc actcccattg
     3121 gtgcttcata gctcatcgac cggatgtccc aacgcagatt tgagattgcg ccgagcatca
     3181 tcacaatcga ccgcaagccg cattctccgg cttcttcaat caattccggt tcgatttcca
     3241 aaattccctt gatatctttc cgtttcaaaa gatcaaccaa ttttttgtca aaaattcttc
     3301 cttgtggaga atatccggct ggcgacccgg gagacacacg atgggacaaa tcgctgctcg
     3361 caataatcga tatgacgcgt ggagattctt gcaaaacttt tcccaacacc cggccgaagt
     3421 tgaaatgata ttccaaagga agcatagaaa aagacatatg gatcaatttt ggattgtagg
     3481 ccgtgtcttt ttcactcaca agatagtgaa gcggcaccag cgctccccga tcaagctcaa
     3541 gttctcgcac cagaccgacc ggcaagcgcg actctcgagc aagtttcata atctcgtcga
     3601 ccagttcccg gttgttttta aaaacaaacc ttcgctggtc tccaaacgaa cccagatctc
     3661 cccgaagggt cggggaatag ttgatcataa atttgtcata ctgtaccggc ccgtgcggag
     3721 aaatcagaac gatcgtatcc gctttcaccg cccgaaattt ttccccaatc tgtttcatcg
     3781 cgttggcggt tttgcgcacc aagtttaaat tgcttttccc gatggatggg acaatgacag
     3841 tgggatgggg agtgatggca gagaaacgaa tcataagatt aaaatagttg ccttaacaaa
     3901 ggatctttga tagaatttta gcatagaaat gagactttat aaagaaagtt tattacaaat
     3961 aactctcaaa aaatgtacaa aaatctgaaa aaacaagatc cggcgatcta caaagccatt
     4021 ttggccgaaa caaaaaggca agaagaaggg ttggaactta tcccttcaga gatcgcggct
     4081 tcggcggccg tgatagaggc tttgggatcg gttttgacca acaaatatag cgagggttat
     4141 ccccggaaaa gatactacgg cgggcaggaa aatatcgata ctattgagga aatcgcccag
     4201 gagcgggcca aaaagctctt tggggtacct catgccaatg ttcagccata ttcaggcagt
     4261 ccggccaatc tggccgtcta tgtggctgta tgcaacccag gcgacgttgt gctgggacaa
     4321 aacctgccgg atgggggaca tttgacccac ggctggaaag ccagtgcgac gagtatgttc
     4381 tacaaaacaa tcggctatca cgtgaatgaa gacggatatc tcgatcttga ggagattgaa
     4441 cggctggcaa aaaagaacaa gccgaaattg atctgggtcg gcgccaccgc ctatgtccgt
     4501 gaatttcctt ttgaaaaaat ggccaagatc gcggacaaag ttggagccta tcttgcggcc
     4561 gacatcgccc acatcgcggg actagtcatt tccggcgcgc acaaaagccc ggcgaagttc
     4621 gcgcatatta ttacgacgac aacccacaaa actttgcgcg gaccgcgggg aggaatgatt
     4681 atggtgacgc ataagggact caaaaaagat ccggatctcg ccaaaaaaat cgatcaagcc
     4741 atttttccgc gcctccaggg cggaccgcac gaccatcaaa cggcggcgat tgccgtcgct
     4801 ctcggtgaag cgatgaaacc gtctttcaaa acatacggcc accaagtcgt gaaaaacgca
     4861 aaagcccttg ccaaagccct gatggcccgt ggagtgaaaa tcgtgaccaa tggcacagac
     4921 aaccatatga tcctcatcaa gtgcggcaaa ggccggggag tttttttgca ggatgcgctc
     4981 gacgccgccg gaatcaccct caacaagaac acaatcccga aagatccgtc ttcacccttc
     5041 tatcccagcg gagtgcgcct cggaacaccc tttatcactg tccgcggaat gaaagaaaaa
     5101 gaaatggaga aactggccga ctggatcgcg caagtgatgg aagagatcaa ggattttcaa
     5161 tttccgtccg acaaagccaa gatcaaacca acactcatca aattcagaaa atttgtcgct
     5221 gggaacaaag agctgaagaa aatcaaaagc gaagtgaaga aactctgcgc caaatttccg
     5281 ctatataaag acttaaaata attttcaatt tcaaaattca aaaatttaga cattgtttga
     5341 aaattgaaaa ttgaaaattg aaaatttgat gaaactacta aacggcacca aactagccaa
     5401 caagattatt cttcaattaa agaataaagt cgcaatcatg acccgcaagc ctgttttggc
     5461 gatggtcttg gttggcaacg acccggcgtc aaaaatatat gtcggccgca aaaaaatcct
     5521 ctgcgaaaaa gcggggatcg gctcgcagat cttcactttt cccgaaaaaa cttccgagaa
     5581 aaagattctg gaaataatcg acggtctcaa caagagccaa aatatttccg cgatcatggt
     5641 ccaactgccg ctcccgaagc atattgacaa ggataagatc attgaagcga ttgacccgcg
     5701 aaaagacgca gattgcctcc atccgctgaa cttcggaaaa ttcacgcaag aaggggaaaa
     5761 atattccgtt gtcgctccag ccacgccgct cggaatcatc aaactgctcg aagaatataa
     5821 agtgaagatc gaagggaaaa atgccgtgat tgtcggacgt tcgaatattg tcgggaaacc
     5881 gatggcgcaa atgctcctca accgcaatgc gaccgtcacg atttgccatc ggcataccaa
     5941 aaatttgaaa agctataccc aaaaagcgga tattctcgtc gtggcaacag gaaaaatgaa
     6001 tatgatcaca gccgatatgg tgcaaaaagg cgtagtggtg attgatgtgg gagtgaaccg
     6061 cggcgaaaac aaaaaagtct tcggcgacgt cgatttcaaa tccgtttcca aaaaagcttc
     6121 tttcatcacc cccgtccccg gcggtgtcgg cccgatgacg attgcaatgc tgctttggaa
     6181 tacggtgaaa ttaaccgaga aataaaagcg tttcaaaaac tccctctgtc ggggggagct
     6241 tttttatttc ccatcaatga attctgtatc ttcaacggtt tcccgtttgg attttttgcg
     6301 ggatagcaat tctttgacca agaccaaatc tccggcgccg atttcacgga gaatataaag
     6361 aataattaaa tatattccga aaagcaaaac tgaccagaga atgaaatagt aattatttcg
     6421 cgggaaaacg aaagcgagag ccgacatcgc ggctgaagct aaaattattt tgaaaaaact
     6481 cgaagcttta aacaaatatc caaaagaccg atgggtataa accagaccaa aaatcattac
     6541 ggcgagagcc ataaacgctg ttccaccggc cgctcccagc aggccatagg atttgatcaa
     6601 aaaataggtc agaaaagtat tgagaaaaag gccgaggatg gccacataca tcggaatttt
     6661 cacttttccg gcgccgttca ggatgaaagt gagaacataa aaaactgtca ggaatccttc
     6721 cgcgatcaca tagacggaca tcacgggcgc ggaaagcgcg tattttgagg aaaaaaatat
     6781 ttgaatgatc ggcagggaaa aataggacat caagaggcac accgggacaa gaaacatcac
     6841 caaaaatcgc agcgactggc tgatgatttt ttttgtttcg tcaaaattgt tcgctgccgt
     6901 tgatttcgag atcgagggca ggaggatgat cgtgagagca taaaaaagat tgtagggaat
     6961 ggtgcccact gtaatcgcgg cgttgtatat tcccgtctga aaatcggaac gcaaaattcc
     7021 tttcacgaga taaaggtcaa tggtgttgag gagctgatag gcgatgagaa aaatcgtcac
     7081 cggccaggca aaaacaacca gctttttcca atcaaatgtc ccaacggatt tcaatttttt
     7141 gtacggatca agcgaccagg cttcgaagaa aatagaaagg ggagcaacga catatcccaa
     7201 aattgctccc tgaagcgcca ttccatgcga tttgagcaaa aacgccagac cgataatgaa
     7261 aacaatccgc aggatgcccc ggttcgtttt gagaaaagcc tggatcttga atttttggat
     7321 gccggtataa taggagaaat agaaa
//
LOCUS       DATKGS010000040         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_205977, whole genome shotgun sequence.
ACCESSION   DATKGS010000040 DATKGS010000000
VERSION     DATKGS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205977"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1260
                     /gene="dnaN"
                     /locus_tag="VMQ48_00970"
     CDS             112..1260
                     /gene="dnaN"
                     /locus_tag="VMQ48_00970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HUD08642.1"
                     /translation="MKAICTTENLKKGILVAEKIIGKNLTLPILSNILLEAEKGGLKI
                     SATNLEIGLIAKVRAKIEKEGKIAVPGRIIGGILSQLDDGSQITLEEKNSTLKMIYNG
                     NSAAIKGMDAKDFPIIPKPESKFLFEMSAAELQKKAGNIVATAATSDTRQELTGIHFE
                     FGETKITLAATDSFRLSEAVIKLDKAKITEDYKKYIAKNKAVIVPARTLSEVIRAISA
                     EDENVKVYLAENQIFFETADVLFVSRLIDGKYPEYKQVVPKEFSLNVIAKKDDLLKAA
                     RLAGVFSDSKSREVRLKIESDKGKIAVFAESVESGEGSWNVDCQVDAKSNLEVAFNNR
                     FLLDGLNSISTEDISISANDSFGPVMLREVADGQPQPDYFHIIMPIRS"
     gene            1274..1870
                     /locus_tag="VMQ48_00975"
     CDS             1274..1870
                     /locus_tag="VMQ48_00975"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUD08643.1"
                     /translation="MQKQVIVIHGGDSYMSREKFLDALKKWKVTRESFLPKYDWKSQL
                     QSELGEEYEVLSPRMPNKQNAKYDEWKIWFERTLPFLRDEVILVGHSMGGLFLAKYLS
                     ENKFPKKIGMFFLAAPPYRDTEDIANFPITEDLQKVWKQCQNIHLFYSTDDPLVPMTE
                     AKEYQKAWPGAKMHVFTDRGHFKQESFPELVAEIRRSA"
     gene            1880..2587
                     /locus_tag="VMQ48_00980"
     CDS             1880..2587
                     /locus_tag="VMQ48_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUD08644.1"
                     /translation="MEIFNPEKAKPKKYLPEVRTIIDIINNEGKKEEDKNFLKNREKD
                     LTSMSEKKETLRHSQRMTNLGYLLAKKMNFSDEETKFFVEACLLHDIGKTAVPRKYLG
                     KPSKKFSDKDLKVVSEHSVLGYKYLKDKGRNSRVYNPILLHHEFQTRPYPHTKASILR
                     ILGAMDDTDIDNGRLLAIIDNFDTQAFGRSYVDIKSAPLGEVKKWIQVEAGFNQPGDE
                     EIIDFLCDQYEKIKELS"
     gene            2611..2745
                     /locus_tag="VMQ48_00985"
     CDS             2611..2745
                     /locus_tag="VMQ48_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08645.1"
                     /translation="MAAEWVEQGDRKLADLIKAYKNKLLVVVFYAEWCPPCLGSNIAA
                     "
BASE COUNT          937 a          577 c          664 g          691 t
ORIGIN      
        1 gggggaataa attcatcaat aaatatataa ttcaatctca cttatcccaa aaaatcccaa
       61 acctaataat aataacaact ttaaataaat aattaaatta ataatataac catgaaagca
      121 atttgtacta cggagaatct aaaaaaagga attcttgtag ccgaaaaaat tattggaaaa
      181 aacctcactc ttccaattct ttcaaatatt cttttggagg cggaaaaggg tggacttaaa
      241 atttccgcga ccaatctgga aatcggcctt attgcaaaag tgcgggcaaa aattgaaaaa
      301 gagggaaaaa tcgcggttcc cggaagaatc atcggcggaa ttttgtccca attggatgac
      361 gggagccaga tcactttgga agaaaaaaat agcaccctta agatgatata taatggaaac
      421 tcggcggcga tcaaggggat ggacgcaaag gattttccaa tcatcccaaa accggaatca
      481 aaatttcttt ttgaaatgag cgcggcggag ctgcaaaaaa aagcgggaaa tatcgtcgca
      541 acggccgcca cttcagatac gcggcaggaa ctcacgggga tccactttga atttggggaa
      601 acaaaaataa ctctggcggc aactgacagt tttcggcttt cagaagcagt cattaaactc
      661 gacaaagcga aaataaccga ggactataaa aaatatatcg ccaaaaataa ggcggtgatc
      721 gtgccggccc gcaccctctc ggaagtcatc cgggcgattt ccgcggaaga tgaaaatgtg
      781 aaagtctatc ttgcggaaaa ccaaatcttt tttgagacgg cggatgtgct ttttgtctcg
      841 cggctcattg acgggaaata tccggaatac aaacaggtgg ttcccaaaga gttttctttg
      901 aacgtgattg cgaaaaaaga cgatcttctc aaagcggcgc gccttgccgg tgttttttct
      961 gattccaaaa gtcgggaagt gcggctcaag atcgaaagcg acaaggggaa aattgctgtt
     1021 tttgcggagt cggttgaatc cggagaggga tcttggaatg tcgattgcca agttgacgcg
     1081 aagagtaatc ttgaagtcgc tttcaataac cgttttcttt tggacgggct caattccatt
     1141 tcaacggaag acatctctat cagcgcaaac gattctttcg gcccggtgat gctgcgcgaa
     1201 gtggcggacg gtcagccgca gcccgactat ttccacatca tcatgccaat tcgaagctaa
     1261 agagtataat cccatgcaaa aacaagtcat tgttattcac ggcggagatt catatatgag
     1321 ccgggaaaaa tttctggacg cgctcaagaa gtggaaagtg acgcgggaat cttttttgcc
     1381 caagtatgac tggaaaagtc agctgcaatc agagcttgga gaagaatatg aagtgctctc
     1441 gccccgcatg cccaacaagc aaaacgccaa atacgacgaa tggaaaattt ggtttgaaag
     1501 gacgcttcct tttttgcgag acgaagtaat actcgtcggt cactcgatgg gcggactatt
     1561 tctcgcgaaa tatttgtcgg aaaacaaatt tccaaaaaag atcggcatgt tttttctggc
     1621 ggcaccgcca taccgagaca cagaggatat cgccaacttt ccaatcacgg aggatttgca
     1681 aaaagtgtgg aagcaatgcc aaaatatcca tctattttat agcacagacg acccgcttgt
     1741 tccgatgacc gaggctaagg aatatcaaaa agcttggccc ggagcgaaaa tgcatgtctt
     1801 cactgaccgc gggcatttca aacaggaatc ttttccggag ctcgtggccg aaatcagaag
     1861 atcagcctaa aatcaaaata tggagatctt caatcccgaa aaagccaagc cgaaaaaata
     1921 tttgccggaa gtccggacca ttattgatat tattaataat gaaggcaaaa aagaagaaga
     1981 caagaatttt ttaaaaaata gagaaaaaga cctgacaagt atgagtgaaa aaaaagaaac
     2041 attgcgccat tcgcagcgca tgactaattt gggatatcta cttgcaaaaa aaatgaattt
     2101 ttccgacgaa gaaaccaagt tctttgtgga agcctgtctt ttgcatgaca ttggaaagac
     2161 agccgttccc cggaaatatc tcggtaagcc ttccaaaaaa ttcagcgata aagatcttaa
     2221 ggtggtgagt gagcattccg tgttaggata taaatacctg aaagataaag ggagaaattc
     2281 ccgggtatat aatccaattc ttctccatca tgagtttcaa acgcggcctt atccacatac
     2341 caaggcgagc atactgagaa ttttgggtgc tatggatgac actgatatcg acaatgggag
     2401 gctccttgct ataattgata attttgatac ccaagcgttt ggacgatcct atgttgatat
     2461 taagtcagca cccctcggcg aagtaaaaaa atggatccag gttgaggcgg ggttcaatca
     2521 gccgggggat gaggagataa tcgattttct gtgcgatcaa tacgaaaaaa taaaagagtt
     2581 gagttaaaat aaaaaaagga ggaagccggc atggccgccg aatgggtcga gcaaggagat
     2641 cggaaattgg cagatctgat caaggcatat aaaaacaaat tgctcgtagt cgttttctat
     2701 gccgagtggt gtccgccttg cctcggctcc aatattgccg cataatacgg atcaggttta
     2761 agcatgtggc gaacttttcc aagagttcgc cactttttct tgaaaaaaag tgggtttggg
     2821 cgtaggatga ggatatgaaa aacatcgagc tcaagatctc ccttccccc
//
LOCUS       DATKGS010000041         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_46873, whole genome shotgun sequence.
ACCESSION   DATKGS010000041 DATKGS010000000
VERSION     DATKGS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46873"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VMQ48_00990"
     CDS             complement(<1..624)
                     /locus_tag="VMQ48_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HUD08646.1"
                     /translation="MIFSKLFGSAEKQTNKFLPLVDEINGFEAEMEKLSDEQLKAKTE
                     EFKKRLKNGEKEDDMLPEVFSVVRESVKRVDGKRLFDVQLLGGITLHQGKIAEMKTGE
                     GKTHTATAAVYLNALSGKGVHIVTVNDYLSKRDMNWMGPIYHFLGLSCACIQHDASFM
                     YELKVHDTDEVSVEMENLRPVSRREAYAADITYGTNNEFGFDYLRDPP"
     gene            complement(902..1597)
                     /locus_tag="VMQ48_00995"
     CDS             complement(902..1597)
                     /locus_tag="VMQ48_00995"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08647.1"
                     /translation="MNSVQTWGEAITLSLLNLWERFINFLPALLGAVLVFVAGWIVAV
                     ALGKAVEHIVKVIRIDDVVEKAGTKGRLRKAGVELNIAKFFGGLVKWFLILVFLMAAT
                     DILHLMQVTSFLNSIVLYLPNVIVAAVILAIAFLIGTFAYAVIKGSTKVAGIVSATLL
                     ATIAKWAIVIFGLLAALVQLGVASSLINTIFIGFVAMLALAGGLAFGLGGKDEAALIL
                     KKLRREITENHGE"
     gene            1692..2252
                     /locus_tag="VMQ48_01000"
     CDS             1692..2252
                     /locus_tag="VMQ48_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08648.1"
                     /translation="MGGKNVFLKAFFKNPRQIGAVAQTSSFVADEMTRSINFSKAKCL
                     VEFGSGMGNMTEKILEKMSPGCILLCFEVDKTLAAKLSQNIQDSRVKIICDSAENLGE
                     HMAKHGIEKADHIISSIPLTTLPYQTVKNILSYSFQYLRSGGHYIQVQYSLATLRQIK
                     YLFSSVAVSFVFLNFPPAFVYVCVKM"
     gene            2355..>2963
                     /locus_tag="VMQ48_01005"
     CDS             2355..>2963
                     /locus_tag="VMQ48_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08649.1"
                     /translation="MDPKIQDFIRLIFKFGRIEIRDVDGGQEPFLYSTKNRGPGYVDV
                     KGGIGIDEFFEPALELLADVVVRDQVPVDVIVGMMSGGALPGYRLKQHLQARLGKRVV
                     YIYQRGARKEGGHGELDTGDRNNPFVSDSCHVLIVEELVNFAGTTTNGVLYERTKRRV
                     VDHAATILFYKNPVAIERLKVNNITLHWVVTLPEVLDLGLSEG"
BASE COUNT          828 a          722 c          609 g          804 t
ORIGIN      
        1 gggagggtca cgcagataat cgaacccgaa ttcgttgttc gtaccgtagg tgatatcggc
       61 tgcgtatgct tcgcgccgtg aaaccggccg caaattttcc atttcgacgc tcacttcatc
      121 cgtatcgtga accttcagct catacataaa tgacgcatcg tgctggatgc acgcgcaaga
      181 gagtccgaga aaatgataga tcggtcccat ccagttcata tcccgcttgg aaagataatc
      241 attgaccgtc acgatgtgca cgccttttcc ggatagcgca ttgagataca ccgctgctgt
      301 cgccgtgtgc gtttttcctt cccccgtttt catttcagcg atttttccct gatgaagcgt
      361 gattccaccc aaaagctgga cgtcaaaaag ccttttccca tccacccttt tcactgattc
      421 tctgaccacg gaaaaaactt cggggagcat atcatcctct ttttcgccat tcttaagcct
      481 ttttttgaat tcttcggttt tcgccttgag ctgctcgtca gaaagctttt ccatctccgc
      541 ctcaaacccg tttatttcgt caacaagcgg cagaaatttg tttgtttgct tttcagccga
      601 tccgaatagt tttgagaaaa tcataatcga tatttatagg caatataata aacttagcaa
      661 ctttctcaat attagcgcaa ttatttgcta atgtaaaatg caaatatcaa aaataaaaat
      721 gacaatttaa aatgtaaaaa tattccattt cgatgaaact atgcttttct atataaaata
      781 attttgaatt ttacactgtc attttacatt ttgactttta aattttgaat tataaaacag
      841 cccaccataa cacagaatgc gagctgcttc agagcccccg taatttaaac aatgcgaaat
      901 actactctcc gtggttctcg gtgatttcgc gccgcaattt tttgagtatc aatgccgctt
      961 catcttttcc gccaagaccg aaagcgagtc cacccgcgag agcgagcatc gccacaaatc
     1021 cgatgaaaat cgtattgatg agcgatgacg ccacaccgag ctggacaaga gccgccaaaa
     1081 gtccgaagat cacaatcgcc catttggcaa tcgtcgcaag aagcgtcgcc gaaacaattc
     1141 cggccacctt tgtgcttccc ttgatgaccg cataggcgaa agttccaatg aggaatgcaa
     1201 tcgccaaaat caccgccgcc acgatcacgt tcggcagata gaggacaatg ctgttgagaa
     1261 agctcgtcac ctgcatcaga tgcaggatgt ctgtcgccgc catcaaaaac accaggatca
     1321 aaaaccattt gacgagtccg ccgaaaaatt tggcaatatt gagctcaaca cccgctttgc
     1381 gcagccgtcc tttcgttccc gctttttcaa ccacgtcatc gatccggatg accttcacaa
     1441 tatgctccac cgcttttcca agcgcgaccg ccacgatcca accagcaaca aaaaccagga
     1501 ccgcccccaa aagagccggc aaaaaattga tgaaccgttc ccagaggttg agcagggaca
     1561 gtgtgatcgc ttctccccaa gtttgaactg aattcatttc ttcacctccg ttctattttt
     1621 gtgtttatat gctatacttt gattataaca gaattgggat aaagataata actcgttgtg
     1681 gataacttca tatgggaggg aaaaatgttt ttttgaaggc tttttttaaa aaccccagac
     1741 agatcggagc agtcgcgcaa acatcttcat tcgtggctga cgaaatgacc agatcaataa
     1801 acttctccaa ggccaagtgc ctggttgaat ttggatcagg gatgggaaat atgaccgaaa
     1861 aaattctgga aaaaatgtct ccaggatgta ttcttctctg ttttgaagtg gataaaacac
     1921 tggccgccaa actctcccaa aatattcaag attcccgggt aaagattatt tgtgacagtg
     1981 ctgaaaactt gggcgaacac atggcaaaac atgggattga aaaagcggat catataattt
     2041 caagtattcc cctcacaact ctgccctatc aaactgtcaa aaatattctt tcttattcct
     2101 tccaatatct tcgaagcggc ggccattata ttcaagtcca atattcactt gccactctcc
     2161 gccagatcaa atatcttttc tcttccgtcg ccgtttcctt cgtcttcctc aacttccccc
     2221 ccgccttcgt ctacgtttgc gtcaaaatgt gaaatgtgat agaaaataaa caggttacac
     2281 gggaggcaac agcctctttt ttgtaaattt ctttgaaaat taaaataacc gatatcaaat
     2341 aaaggaggta ggacatggat cccaaaattc aagactttat ccgcttaata tttaaattcg
     2401 gcagaattga aattcgcgat gtggacggcg gtcaagagcc gtttctctac agcacgaaaa
     2461 acaggggacc gggttatgtc gatgtaaaag gaggcatcgg cattgacgaa ttttttgaac
     2521 cggctttgga acttcttgcc gatgtcgtag tgcgagatca ggttcccgtg gacgtgatcg
     2581 tcggaatgat gtccggcgga gcattgcccg gatatcgtct caagcaacat ctccaagccc
     2641 gtttaggaaa gcgtgttgtg tatatttatc agcgcggagc caggaaagag ggcggtcatg
     2701 gcgaactcga caccggcgat cgcaataatc cgtttgtatc ggacagctgc catgtcctta
     2761 tcgtcgagga actggttaat tttgccggta ccacaactaa cggcgttctc tatgagcgga
     2821 cgaagagacg agtcgttgat cacgcggcca ctatactttt ttacaaaaat cccgtggcga
     2881 tcgaacggct caaagtcaac aatataactc ttcactgggt cgtaacgctt ccggaggttt
     2941 tggatctagg cctttcggaa ggc
//
LOCUS       DATKGS010000042         5210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_31165, whole genome shotgun sequence.
ACCESSION   DATKGS010000042 DATKGS010000000
VERSION     DATKGS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31165"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VMQ48_01010"
     CDS             <1..965
                     /locus_tag="VMQ48_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08650.1"
                     /translation="FAQTDCEGGFDSVIFDKKFIIDNNDQLTNYLESIMESSKNIAIK
                     DRITHAIFTGAAVMLVVFLISLALPKQWLVSGRIVVFPSGQPASASQNLLPETGNTAE
                     IIKSVSFQKANFADQSANFAGARAVRNSSMVQVSFRSSENDIQSYEDLIVKIPGQVND
                     YARDLYDGSPFKYKMAGDPEISTSPVRPNLLLNAILGFVGGIVLYVIYMLGFESREPK
                     IEVEEVEIKEKKSSIVSPEIKPQIIAESVSLTVKKKEEKIGNGKVEMKDGRPHFVPGK
                     PEEKKSSAPENLPFVDELNSSSNIQEPSDDEVKERLNRLMRGEL"
     gene            1052..1603
                     /gene="rfbC"
                     /locus_tag="VMQ48_01015"
     CDS             1052..1603
                     /gene="rfbC"
                     /locus_tag="VMQ48_01015"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HUD08651.1"
                     /translation="MEFVETSLKGAYTIKPKVFQDERGFFLETYSKKVFAEHGIEADF
                     VQDNHSLSVKKGVLRGLHFQLPPNDQAKLVRVTRGKVYDVIVDIRKNSPTYGKWEGFE
                     ISGDNFQMLFIPRGFAHAFLTLEDNTEFMYKVDNFYAPESDSGIIWNDPDLKIDWPIE
                     NPVLSDKDTKLQKFKDFNSPFKL"
     gene            1600..2616
                     /gene="rfbB"
                     /locus_tag="VMQ48_01020"
     CDS             1600..2616
                     /gene="rfbB"
                     /locus_tag="VMQ48_01020"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HUD08652.1"
                     /translation="MKLLVTGGAGFIGSNFVRYIFNKYPDYKIVNLDLLTYAGNLENL
                     KDVETNPNYKFIKGDIADKELVNNLVKDCDVIVNFAAESHVDRSILDSSDFIRTNIVG
                     THNLLEAAKNNGLKRFHHISTDEVFGHLGPNDPAFDENTPYAPRSPYSASKAASDHLV
                     RAYFHTFGLPITISNCSNNYGPFHFPEKLIPLAITNIIEGKKIPVYGDGLQVRDWLFV
                     EDHCEAIDLILQKGKIGETYCIGGNSEKTNMEIVKKILELLGKGEEMIEYVKDRPGHD
                     RRYAIDFSKIKNELGWQPRTSFEDGMRKTVEWFESNESWWRDVKSGEYQKYYEKQYSN
                     NIQC"
     gene            2652..3521
                     /gene="rfbD"
                     /locus_tag="VMQ48_01025"
     CDS             2652..3521
                     /gene="rfbD"
                     /locus_tag="VMQ48_01025"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HUD08653.1"
                     /translation="MAKRILIIGSHGMLGQDLVRVFERDMNWEVFAYDRDQIDIASVE
                     SLRGKIEEVGPDAVINASGYNAVDKCESDEIEFELAKKINGYGPGMLAKICADMEIPV
                     IHYVSDYVFDGEKGEYAETDKPNPISNYGRSKLLGEEEIQKNTDKFYLIRTSKLFGKP
                     GKSEMAKKSFFDTMIGLAKDNSVLKIVDEEKSCFTYTPDLATETKKMIEEKVPFGIYH
                     VVNEGACTWYEAALELFRLAKMDVEVIPVSSSEFPRPAKRPHSSVLLNTKLKPLRNYK
                     EALKEYLKENYSM"
     gene            3570..4379
                     /locus_tag="VMQ48_01030"
     CDS             3570..4379
                     /locus_tag="VMQ48_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUD08654.1"
                     /translation="MSNKMKGIILAGGTATRLFPLTATTSKQLLPVYNRQMIFYPLNV
                     LIKAGIKDILMIVSPEHSGQFLNLLGRIFERHDVRLEFKVQSVPRGLADAFILGENFI
                     DNDNVTMILGDNIFEDDFSRVIKNFRSGGQIFAKKVPDPERYGVVKFDKSGKAVQIVE
                     KPVDWISDYAIPGLYIYDSRVCLAAKNAKPSERGEKEITSIHNYYLEKGVLQVSTFEG
                     EWLDAGTFDTLLEAGKIVKEKEIYKNFDPQIEKAIEEFNAEMKTIAKKQLL"
     gene            4401..>5210
                     /locus_tag="VMQ48_01035"
     CDS             4401..>5210
                     /locus_tag="VMQ48_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HUD08655.1"
                     /translation="MKIKKAIVAVAGSGTRFLPVTKAMPKEMLPIVDKPIIQLVVEEL
                     VEAGIEDIILVTKWDKKPLEDHFDHSWALENELEKAGKTERLNAVKRVADMANFIYVR
                     QKGPYGNATPVLSAASLVDNEPFVFVWGDDLVKSKVSFTKQMVEDYEKNGKLMIGVQE
                     VPKDAVSRYGIVKLREGTDEIEDIVEKPSIEEAPSQLADFGRMILNQEIIDVLKKTDL
                     GKGGELWITDAIQNYVRNGGIFVAKKIEDGEWLTTGDPLNYLKAVLAYAAER"
BASE COUNT         1618 a         1026 c         1276 g         1290 t
ORIGIN      
        1 aatttgcgca gactgattgt gaagggggat ttgactcagt gatatttgac aaaaaattca
       61 ttatcgataa taatgaccaa ctaacaaatt atcttgaaag tattatggaa tcttcgaaaa
      121 acatcgccat caaagatcgg atcactcatg caatcttcac aggagccgct gtaatgcttg
      181 ttgtattttt gatttcactg gctcttccca agcagtggct tgtgagcgga agaattgtgg
      241 ttttcccttc cggacagccg gcgagcgctt cgcagaatct tttgccggaa acaggaaata
      301 cggcagaaat catcaaaagc gtctcttttc aaaaagcgaa ttttgctgac cagtctgcga
      361 actttgcggg ggcgagagcc gtgagaaatt cttcaatggt ccaggtgagc tttcgcagct
      421 ccgagaatga tatccaatcc tatgaagact tgatcgtgaa aatcccgggt caagtcaatg
      481 attatgcccg cgacctgtat gacggttcgc ctttcaaata caaaatggcg ggcgatccgg
      541 aaatttccac aagccctgtt cgacccaatt tgctgttgaa tgccattctt ggattcgttg
      601 gtggtatagt gctttatgtg atatacatgc ttggatttga atcaagagaa ccgaaaattg
      661 aagtcgaaga agtcgaaatc aaagaaaaaa aatcatccat agtttcgccg gaaatcaagc
      721 cccaaattat agctgagtcc gtctcattga ctgtcaagaa aaaagaagaa aaaataggca
      781 atggcaaagt ggaaatgaaa gacggccggc cgcatttcgt gccgggaaaa ccggaagaga
      841 aaaaatcttc cgcaccggaa aacctgcctt tcgtggacga attgaattct tcttcaaaca
      901 ttcaagaacc ttcggacgat gaagtgaaag aaagattgaa tcggttgatg cgaggggagt
      961 tgtaattgca aaaagaattt atatagttaa aaagacccgt tgtctcgggc ctttttgatt
     1021 tttttgagga aatgagataa aattaagttg catggaattt gtcgaaacaa gtctaaaagg
     1081 cgcatatacc atcaaaccca aagtctttca ggacgaaaga ggtttttttc ttgaaacata
     1141 tagcaaaaaa gtttttgccg agcatggaat cgaagctgat tttgttcagg acaatcattc
     1201 tttgtcagtg aaaaagggag ttttgcgagg gctgcatttc cagctgccgc cgaatgatca
     1261 ggcgaaattg gtacgagtga cgcgggggaa agtatatgac gtcattgttg atatcagaaa
     1321 aaattcgccg acatacggaa aatgggaagg ctttgagatt tcgggtgata attttcaaat
     1381 gcttttcatt cctcgcggtt ttgctcacgc ctttctaact cttgaggaca acacggaatt
     1441 tatgtataaa gtggataact tctatgcgcc ggaatccgat tcgggaataa tttggaacga
     1501 tcccgatttg aaaattgact ggccgattga aaatcccgta ttatctgata aagataccaa
     1561 gctgcaaaaa ttcaaagatt tcaattcacc attcaaatta tgaaactact cgtcaccggc
     1621 ggagccggat tcatcggttc caacttcgtc cgctatattt ttaacaaata tcccgattac
     1681 aaaatcgtta atctcgatct tttgacatac gcggggaatt tggagaattt gaaagacgtt
     1741 gaaacaaatc caaattacaa atttatcaaa ggtgatattg ccgacaaaga attggtgaat
     1801 aatctcgtca aagattgcga tgtgatcgtc aacttcgccg ccgaatcgca cgttgaccgg
     1861 tcgatccttg acagttcgga tttcatccgg acaaatattg tcggcactca caatctgctg
     1921 gaagcggcga aaaacaatgg tctcaagcga tttcaccata tctccacaga cgaggtcttc
     1981 ggccatttgg gtcccaatga ccctgccttt gatgaaaaca cgccctacgc gcccagaagc
     2041 ccatacagcg cgtccaaagc ggcttccgac cacctggttc gggcatattt ccatactttc
     2101 gggttgccga tcacgatttc caattgctcg aataattatg gtccctttca cttcccggaa
     2161 aagctgattc cgctggcgat tacgaatata atcgaaggca agaaaattcc cgtgtatggc
     2221 gacggattgc aagtgcgaga ttggcttttt gttgaggatc attgcgaggc gatcgatctg
     2281 attcttcaaa aaggaaagat aggggagaca tattgcatcg gcggcaattc cgaaaaaacg
     2341 aacatggaaa tcgtcaaaaa aattctcgag cttctcggaa aaggcgaaga gatgatagaa
     2401 tacgtgaaag accgacccgg ccacgaccga agatatgcga ttgatttttc gaaaataaaa
     2461 aatgaacttg gctggcagcc gcggacaagc tttgaagacg gaatgcgaaa gacggttgaa
     2521 tggttcgaaa gcaacgaaag ttggtggcgc gacgtgaaaa gcggagaata tcaaaaatat
     2581 tacgaaaagc aatatagtaa caacattcaa tgttagggcg accccaacat tgaatgttga
     2641 ggtaaaaaag catggcaaag cgaatattaa tcattggctc tcacggaatg ctcgggcagg
     2701 atcttgtgcg ggtgtttgaa agggatatga attgggaagt ttttgcgtat gaccgcgatc
     2761 aaatcgatat tgcgtccgtt gaaagtttgc gcgggaaaat cgaagaagtc gggccggacg
     2821 cggtgatcaa tgcgtcggga tacaacgctg tggacaaatg tgaatcggat gagattgagt
     2881 ttgagctggc caaaaagatc aatggatatg gtcccgggat gctggccaaa atttgcgctg
     2941 atatggaaat tccggttatc cactatgtga gcgactatgt ttttgacggg gaaaaggggg
     3001 aatatgcgga aactgacaaa ccgaatccga tttcgaacta tggacggtcg aagctgctcg
     3061 gagaggaaga aatccaaaaa aacacggata agttttatct catccgcacg tcaaaactat
     3121 ttggaaaacc cggaaaatcg gaaatggcca aaaaaagttt ttttgacacg atgatcggac
     3181 tcgcgaaaga caattctgtt ttgaaaatag tcgacgagga aaaaagttgc ttcacttata
     3241 cgccggatct ggctaccgaa acgaaaaaaa tgattgaaga aaaagttcct ttcgggatat
     3301 atcacgtcgt caacgagggc gcttgcacct ggtatgaagc ggcgctcgaa ctcttccggc
     3361 tcgcgaaaat ggatgtggaa gtgattccgg tgagctcaag cgaattcccg cgtccggcca
     3421 agcgtccaca ctcttcagtg cttctgaaca caaaattgaa accgctccga aactacaaag
     3481 aagcgctgaa agaatatctg aaggaaaatt attctatgta gcacgtagag acgaggcaat
     3541 gcctcgtctc tacgatgtga aaataatcta tgagcaataa aatgaaaggc attattctcg
     3601 ccggagggac ggcgacgagg ttgttcccgc tcacggcgac gacttcaaag cagcttttgc
     3661 ccgtgtataa ccgccaaatg atcttctatc cgctcaatgt tttgataaag gcgggaataa
     3721 aagatatttt gatgatcgtt tcgcccgagc actccggaca atttctcaat cttctcggtc
     3781 gcatttttga gcggcatgac gtgcggctcg agttcaaagt gcaaagcgtg cctcgcggac
     3841 tggctgatgc tttcattctt ggagaaaatt tcattgacaa tgacaatgtg accatgattc
     3901 tcggagacaa tattttcgag gatgattttt ccagggttat taaaaatttt cgttccggtg
     3961 gccagatttt tgccaaaaaa gttcccgatc cggaaagata tggcgtggta aagtttgata
     4021 aatcggggaa ggccgtgcaa attgtcgaaa aaccagttga ttggataagc gactatgcta
     4081 ttccgggact ctatatctat gacagtcggg tatgcctcgc cgcgaaaaac gccaaacctt
     4141 cggagcgggg agaaaaagag atcacttcga ttcataacta ttatctcgaa aaaggagtat
     4201 tgcaagtaag cacgtttgaa ggcgaatggc tcgatgccgg aacattcgac acgcttcttg
     4261 aagcaggaaa gatcgtgaaa gaaaaagaaa tatacaaaaa tttcgacccc cagatcgaaa
     4321 aagcaatcga agaattcaac gcagaaatga aaacaatcgc caaaaaacaa ttgttgtaat
     4381 tattattttt tagacaggcc atgaagataa aaaaagccat tgttgccgtt gccggatccg
     4441 gaaccagatt tttgccggtg acgaaagcga tgccgaaaga aatgctgccg attgtcgaca
     4501 aaccgatcat tcagctggtt gttgaagagc tggttgaggc ggggattgaa gatattattc
     4561 ttgtcaccaa gtgggacaaa aagccgctgg aagaccattt tgaccattcc tgggcgctcg
     4621 aaaatgagct ggaaaaagcg ggaaaaacag aaagattgaa cgcggtgaaa agggtcgcgg
     4681 atatggccaa tttcatttat gtccggcaaa aggggccgta tgggaatgcg actccggtgc
     4741 tttcggccgc cagccttgtc gacaacgaac catttgtttt cgtttgggga gacgaccttg
     4801 ttaagtcgaa agtatcgttc acgaaacaaa tggtggagga ttatgaaaaa aatggaaaac
     4861 ttatgattgg tgttcaagag gttcccaaag atgctgtcag ccgatatgga atcgtgaaat
     4921 tgagagaggg aacggacgaa atcgaagaca tagtcgaaaa gccatcaatt gaagaagcgc
     4981 catcgcagtt ggctgatttc ggacggatga ttttgaatca ggagatcatt gatgttctca
     5041 aaaaaactga tctgggaaaa ggaggcgaac tttggatcac ggatgccatt caaaattatg
     5101 tcagaaacgg cggaatattc gtggcgaaaa agatcgaaga cggcgaatgg ctgacgaccg
     5161 gtgatccgct caattatctg aaagctgttc ttgcttatgc ggcagaaagg
//
LOCUS       DATKGS010000043         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_162067, whole genome shotgun sequence.
ACCESSION   DATKGS010000043 DATKGS010000000
VERSION     DATKGS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162067"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VMQ48_01040"
     CDS             <1..1023
                     /locus_tag="VMQ48_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUD08656.1"
                     /translation="NQEKNKLPGQKYIWLIQIVFSVAIVVLIFLNYISIISAILLFFV
                     LAVNSAAFALLLSGFRKGLNFYNTETEQKSKKIEEFDQITRQLVKRELEVAHANQRLA
                     ELDVAKSDFVSVAAHQLRTPLTGIKWSYTALLDPETGPLTAEQKEIAENGLASISNTL
                     DLINDLLNVAHIEEGKMKFEIKRQSIFPVFKKAVEGFRLIADEKKITLAVKIPDESGF
                     PDVDIDAEKMELAITNLLDNAVKYTPNGGRIDFSISQEQGLVRMVVQDSGIGIPKDQK
                     NRLFSKFFRADNATAVQTSGTGLGLYMVKKIIDRHGGKIVVDSTEGKGTAFVITIPEI
                     SAKKGE"
     gene            complement(1029..1562)
                     /locus_tag="VMQ48_01045"
     CDS             complement(1029..1562)
                     /locus_tag="VMQ48_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HUD08657.1"
                     /translation="MSRTNPYKKKKRSAKKTILIFGEGLGEESFLKHLRSLYSQDSGA
                     HAKIQKGRGGAAERIVVDSHKTPGDFDKRFVVVDNDKTKEEMERARQGAKKRGIELIE
                     NTPCLEFLLLLILKNGQKISERSSSWCKKEFESKYIDKKKRSESREYAKLFPRELLDD
                     KRHEISELDKLISILEG"
     gene            complement(1575..>2443)
                     /locus_tag="VMQ48_01050"
     CDS             complement(1575..>2443)
                     /locus_tag="VMQ48_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD08658.1"
                     /translation="SEKKILSEELKIKNKTKEKTTSKKVFFRKWDEESGKYELEDSNF
                     DLPKGFENLLRTNASVIGVALRLNHKGSQKISNFWQQVETNVIEAGWIGDHLLPNTSK
                     LMIETLHFFSESENEALKKEAEKLLSRFDLGLESFDIKKEKKENELSINVQVAHTFGD
                     EKIYLPMQYESSGTKQLFVLLKAILVVLAKGGVAVLDEFDVNLHPEMVLSLFDLFVQP
                     ETNPKNAQLLFSTHSHRVLSKLDKYQIILTEKNEKGGSEAWRLDDVSGVRADDNFFSK
                     YIAGAYGAVPKF"
BASE COUNT          648 a          559 c          489 g          747 t
ORIGIN      
        1 aatcaagaaa aaaacaagct tccaggccaa aaatatatct ggctgatcca gattgttttc
       61 agtgtcgcca ttgttgtttt gatatttttg aactatatct caattatttc ggctattctt
      121 ctttttttcg tcttggccgt caattcagcg gccttcgcgc ttctcctttc cggtttcaga
      181 aaaggattga atttttataa cactgaaact gaacagaaat caaaaaaaat agaagaattt
      241 gaccagataa cccgccaatt ggtcaagcgg gaattggaag tcgcccatgc caatcagcgc
      301 ctcgccgaac ttgatgtcgc gaaatcagac tttgtttccg tcgccgccca ccaattgcgc
      361 actcccctca caggaatcaa atggtcgtat acggcgcttc ttgatccgga aacaggaccg
      421 ctcaccgccg agcaaaaaga aattgccgag aacggtctcg cctcaatcag caatacgctt
      481 gatttgatca atgatcttct caatgtcgcc catatcgaag agggaaaaat gaaattcgaa
      541 atcaagagac agtctatatt tcccgttttt aaaaaggccg tcgaaggttt caggttgatc
      601 gctgacgaaa aaaagattac cttagctgtg aaaattccgg atgaatccgg tttcccggat
      661 gtggatatcg atgcggaaaa aatggaattg gccatcacaa atcttctgga caatgcggtc
      721 aaatatacgc cgaacggcgg aaggattgat ttcagtatca gccaggaaca agggctcgtt
      781 cgaatggttg ttcaggatag cggaatcgga attcccaagg atcagaaaaa caggcttttc
      841 tcgaaatttt tccgggccga caatgccacg gctgtccaga cctccggcac tgggctcggg
      901 ctttatatgg tgaaaaaaat aatcgaccgc catggaggaa aaatcgtggt ggacagcacc
      961 gaaggcaaag gtactgcctt tgtcatcact attccggaga tttcagcgaa gaagggtgaa
     1021 taaagttctc aaccctccag aattgaaatc aatttgtcca attctgaaat ttcatgtcgt
     1081 ttgtcatcca acaattccct tgggaacaat ttggcatatt cccttgattc gcttcgtttt
     1141 ttcttatcaa tatattttga ttcgaactct ttcttgcacc agctcgaact tctctcacta
     1201 attttttgcc cgtttttcaa aatcaaaagc agcaagaatt caagacaagg tgtgttttca
     1261 atcaactcaa tacctctttt cttcgctcct tgtctggctc tctccatttc ctctttcgtt
     1321 ttgtcgttgt caaccacaac aaaccttttg tcaaaatcgc ccggagtttt gtgggaatca
     1381 acgacaattc tttcagctgc acctcccctg cccttttgaa tcttggcatg cgccccgcta
     1441 tcttgagaat acaaacttct cagatgttta aggaagcttt cttcgcccaa tccttcccca
     1501 aaaatgagga ttgttttttt ggctgatcgc tttttctttt tgtatgggtt tgttcgagac
     1561 atttttgatg caagctaaaa ttttggaaca gctccgtatg ctccggcaat atattttgag
     1621 aagaaattat cgtcagcgcg aacaccggac acatcatcaa ggcgccatgc ttcgctcccg
     1681 cctttctcat ttttttcagt taagattatc tggtatttat caagcttgct caaaacccga
     1741 tgactgtgcg tgctgaacag tagttgggcg ttttttggat tggtttccgg ctgaacaaat
     1801 agatcgaaca aagacaagac catttccgga tggagattga catcaaattc atcgagtacc
     1861 gcaacgccgc ctttcgcgag aaccacaaga atggctttga gcaaaacaaa gagctgcttg
     1921 gttccggatg attcgtattg catcggaaga tatatttttt catcgccgaa agtatgggca
     1981 acttgcacat taatagagag ttcgttttct tttttttcct tttttatgtc gaatgattcc
     2041 aacccgagat caaagcggga caaaagcttt tcagcttctt ttttcagagc ttcattttca
     2101 ctttcgctga aaaaatgcag ggtttcgatc ataagtttcg aagtattcgg cagcaagtga
     2161 tcgccgatcc aaccggcctc aatgacattt gtttcaactt gctgccagaa atttgagatc
     2221 ttctgacttc ccttgtgatt gaggcgcaga gccaccccaa tgacgctggc atttgttcga
     2281 agcagatttt caaatccctt gggaaggtcg aaattgctgt cttccagttc gtattttccg
     2341 ctttcttcat cccatttccg aaaaaaaact tttttgctgg ttgtcttttc tttcgttttg
     2401 ttttttattt tcagctcctc ggacaaaatt ttcttctcgg aaa
//
LOCUS       DATKGS010000044         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_16815, whole genome shotgun sequence.
ACCESSION   DATKGS010000044 DATKGS010000000
VERSION     DATKGS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16815"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VMQ48_01055"
     CDS             <1..261
                     /locus_tag="VMQ48_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08659.1"
                     /translation="VFRKTDDYEMSYYPTDQGFIITLQNSDLTNARTAAEKDFIDALG
                     ISQEQSCFLKVTLNVPFDVSQAASGRNYGLSFCSNGKPLPKK"
     gene            273..1460
                     /locus_tag="VMQ48_01060"
     CDS             273..1460
                     /locus_tag="VMQ48_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08660.1"
                     /translation="MSKKIFAISTLLLVLVVGAIFVYNFAFKKPAASTNSNADKATAK
                     TAQEGMTPDKTTAQADTPKTSPTSNLSVSAVSDEPVFGAALSADGNSLYYFLRNNGQL
                     NQADLNGKLEKVLSTEKFTNLKKITWNKPKNKVIIKTESAPGKSKFLYFDIPGKKVSV
                     LKENLDSVAWSNLGDKIIYKYYDPKTKKRTVSTADPDGKNWTDLTEFNYQGVEISPIP
                     SSSDISFWPSPNAFTPTSVNSISFGGGAKKEILKDKFGADLLWSPDGSQAAVSFTDQK
                     GGHKTELALMNSQGGEFQSLGFATFATKCAWSTDSKYLFCALPGNIPDTAILPDDWQA
                     SKISTSDTFWKVEAATGKKDRLVDPEKIGGTYDALFPFLSKDEKSLFFVNKADGKLYK
                     LDF"
     gene            complement(1466..>2315)
                     /locus_tag="VMQ48_01065"
     CDS             complement(1466..>2315)
                     /locus_tag="VMQ48_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUD08661.1"
                     /translation="KSWLALLFSGVAVSMMGIVYKFSGLLTYDGRLHIFYDSPNQLGM
                     FLAPIFIVGLVLFLRENLYWRKLLFLAGLFLLAVNLYFTYSYGTWMALAVAITVVFWL
                     KHKTLRSRVGILILVFLAAIAIGQMRSQKMADLENMGGRSSIDSRIMIWKAAGLMIES
                     NPLFGIGPGNFQNKYLEYQKYFPPYLEWAVPQPHNLFLAFWLEAGFLGLVGFVFLLVQ
                     FFKDNKKAIENNHLYGTLCFAVMLYLLVHGLIDTTYWRNDMAIIFWTIISINTFLSSK
                     GKAQGD"
BASE COUNT          726 a          575 c          461 g          553 t
ORIGIN      
        1 gtcttccgaa aaaccgatga ctatgaaatg tcatactacc cgaccgacca gggatttatc
       61 atcactctcc aaaattccga tctgacaaac gcgagaacag ccgccgaaaa agattttatc
      121 gacgcccttg gcatcagtca agagcagagt tgttttttga aagtgactct caatgtcccg
      181 tttgatgtaa gtcaagctgc ttccggccga aattacggct tgagtttttg ctcaaacgga
      241 aaacctcttc caaaaaaata atcgaaagac taatgtcaaa gaaaatcttc gctatttcca
      301 cgcttcttct agttctcgtt gtcggagcta tttttgtgta taattttgcc tttaaaaagc
      361 cggccgcctc gaccaattcg aacgctgata aagcaactgc caaaaccgct caggagggaa
      421 tgacccccga caaaacaacg gctcaagccg acacaccaaa gacatctccg acaagcaatt
      481 tatccgtttc cgcggtttcc gacgaaccag tttttggagc ggccctctcc gccgacggaa
      541 attcattgta ttattttttg agaaacaacg gccagctcaa tcaagctgac ttaaatggaa
      601 aactggagaa ggttctttcg acagaaaaat tcacgaatct caaaaaaatc acctggaaca
      661 aaccgaaaaa caaagttatc attaaaacag aatcggctcc cggaaaatcg aagttcctat
      721 atttcgatat tcccggaaaa aaagtttcgg tcctcaagga aaatctcgat tcggtcgcct
      781 ggtcgaatct gggagacaag atcatttata aatactacga cccgaaaacc aaaaagcgca
      841 ccgtgagcac tgccgatcca gacggaaaaa attggaccga cctgaccgaa ttcaattacc
      901 aaggcgtgga gatcagccct attcccagct cttccgacat ttccttctgg cccagtccca
      961 acgcctttac tcccacttcc gtcaattcga tttctttcgg cggaggagcc aaaaaagaaa
     1021 ttctcaaaga caagtttgga gcggatctcc tctggtctcc cgatggctcc caagccgcgg
     1081 tgagtttcac cgaccaaaag ggaggccaca aaacggaact ggcgcttatg aattcccaag
     1141 ggggagagtt tcaatccctc ggctttgcaa cttttgcgac gaaatgcgcc tggtccaccg
     1201 attcaaaata tcttttctgc gcccttcctg gaaatattcc cgatacggcc attctgcctg
     1261 acgactggca agccagtaaa atttcaacca gcgatacttt ctggaaagtg gaagcggcga
     1321 ccggcaagaa agaccgcctt gtcgatccgg aaaagatcgg aggaacctac gacgcccttt
     1381 tcccttttct ctcgaaggat gaaaaaagcc tgttttttgt gaacaaggcg gatgggaagc
     1441 tctataaact agatttttaa ttgttctaat cgccctgagc cttcccctta cttgaaagaa
     1501 aggtgtttat actaattatt gtccaaaaaa taatcgccat atcatttctc cagtacgttg
     1561 tgtcaatcag tccatgaaca agcaaataga gcataacagc aaagcatagc gttccataca
     1621 agtgattgtt ttcaatcgct tttttgttat ccttaaaaaa ttgaactaat aaaaaaacaa
     1681 aaccaacaag acccaaaaat cccgcttcta gccaaaaggc cagaaacaaa ttatgcggct
     1741 ggggaaccgc ccactcaaga tacggcggga aatatttttg gtattccaga tatttgttct
     1801 ggaaatttcc cggtccgatt ccaaaaaggg gattgctttc tatcatgaga ccggctgctt
     1861 tccagatcat tatccgcgaa tctattgatg aacgcccgcc catattttca aggtctgcca
     1921 ttttttggct tctcatctgt cctatggcaa tggcagccaa aaagaccaga atcaagatac
     1981 cgactcgcga cctaagagtt ttatgcttaa gccaaaatac gactgtaatg gcgactgcta
     2041 gcgccatcca ggttccatag gaataggtga aataaaggtt gaccgccaaa agaaaaagcc
     2101 cggccaagaa taacaatttg cgccaatata aattctctcg caaaaaaagg acgagtccga
     2161 caataaaaat cggcgcgaga aacataccca gttgattcgg cgaatcataa aaaatatgca
     2221 gcctcccgtc gtaggtcaag agaccggaaa atttataaac aatgcccatc atcgatacag
     2281 ccacaccgga aaaaagcaaa gcgagccaag acttg
//
LOCUS       DATKGS010000045         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_189441, whole genome shotgun sequence.
ACCESSION   DATKGS010000045 DATKGS010000000
VERSION     DATKGS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189441"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VMQ48_01070"
     CDS             complement(<1..723)
                     /locus_tag="VMQ48_01070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08662.1"
                     /translation="MKERIITVLIFLAIVAASFYFGFHRLESYSGVDEPLWSYGRVPQ
                     FWNAIQKMKWKSTTLCDKPGVTLAAISGIGLNWVNGDPTKLGDLRYAPKTPQDLQALR
                     GLYFDLRLPVYLFTLAILPVFYWLIKRLLGKQIARASIIFIGLSPVLLGISLMINADA
                     VLWSLIPLVLLSFLIYQKENNRNFLYLSGFLLGLALIDKYIANILFLFFFGLIFLKYI
                     SEGDAPKAEDYFKKAFADFGLFP"
     gene            complement(726..1673)
                     /locus_tag="VMQ48_01075"
     CDS             complement(726..1673)
                     /locus_tag="VMQ48_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD08663.1"
                     /translation="MENTNQKPDLSVVVPIYNEEGNVRELHQKIVEACQALGKSFEIV
                     FIDDGSKDKTNEICRTLSPLKLIVQRKNFGQTAAFDAGFKNSTGKVVITMDGDLQNDP
                     ADIGKLLEKMDEGYDVVSGWRKYRKDKFLKKLNSRIANLLRKFLIDDGIHDSGCSLKA
                     YKRECFDDLDLFGEMHRFIPANLKVQGFTVTEIPVSHHPRKHGVTKYNWKRGVKGIVD
                     MISVWFWKKYANRPLHLFGAGGLFIFGLGFILTFVLAVAKFAYGVSMSDKNWPLVAVF
                     LLLTGIQLFIFGLLADIATKNYYKAHGGMNYKIKEIIEN"
     gene            1723..>2787
                     /locus_tag="VMQ48_01080"
     CDS             1723..>2787
                     /locus_tag="VMQ48_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08664.1"
                     /translation="MKIFLGILVFFLAFGWLVLLLAATGLFYFGAVVLSIVASAVLAW
                     LFLPKISQNINVKTGKIFYLVILISLLVSLAACHFTTPTVFGGRDQGSIGTAAIYLAQ
                     NHNTKVDLPIANDLFQKYGPGKALNFPGFDYAKNGSLFSRFPLGFTAYLASAYDLFNL
                     KGIQYANFIPLFFFLVIFWLILKEFFDDKVSLLGFFLAASFFPFLWFAKYALTETYML
                     FLVWAGIFFLLSCRRPTSTNLDVRRLQISLAFFGLSALVRIEGIVFFFLAAIYILLLQ
                     RKKILMLPKNFKRNLVIATLVLLGLYVYLNFPDLVDSGKNLVKAFLPNSAKESAPSTN
                     MYEYLARIFFNYNLLAYILIG"
BASE COUNT          718 a          692 c          569 g          808 t
ORIGIN      
        1 gggaaaaagt ccgaaatcgg caaaagcctt tttgaaataa tcttcagcct tgggcgcatc
       61 gccctccgaa atatatttca aaaaaatgag tccaaaaaag aacaagaaga ggatgttcgc
      121 gatatatttg tcaatgagag ccaatcccag caaaaatccg cttaaatata ggaaatttcg
      181 gttgttttct ttttgataga tcaggaaact caaaagcacg agagggatca agctccacaa
      241 aacagcgtcc gcattgatca tcaatgaaat cccgagaagc accggggaca gcccgatgaa
      301 aatgatcgac gcccgggcga tctgcttgcc caacagtctc ttgatcagcc aatagaaaac
      361 gggcagtatg gcgagcgtga agagatatac cggcaggcgc aaatcaaaat acaggccgcg
      421 aagcgcctgc aaatcttgtg gagtcttcgg tgcgtagcgc aagtctccga gtttcgtcgg
      481 atcgccattc acccaattca aaccgatgcc ggaaatggcc gccaaggtga cgcccggttt
      541 gtcgcaaagc gtcgtgcttt tccatttcat tttctggatg gcattccaga attgaggcac
      601 ccggccgtag gaccacaatg gctcgtccac gccggaataa ctttcaagac gatgaaaccc
      661 gaaataaaaa cttgctgcaa caatagcaag aaagatcagg acagtgatga ttctttcttt
      721 catctttaat tttcgatgat ttcttttatc ttatagttca ttcctccgtg cgccttgtaa
      781 tagtttttcg tggcaatgtc agcgaggagt ccgaaaatga agagctgaat ccccgtcagt
      841 aagagaaaaa ccgcgaccaa cggccaattt ttgtccgaca tgctcacacc ataggcaaat
      901 tttgccacag caagcacgaa agtcaaaata aagccgagtc caaagatgaa tagtccaccg
      961 gcgccaaaaa gatggagcgg ccgattggca tattttttcc aaaaccagac ggaaatcata
     1021 tcgacaatcc ccttcactcc ccgcttccag ttgtattttg tcaccccatg tttgcgcgga
     1081 tgatggctca ctggaatttc cgtgactgtg aatccttgca ctttgagatt ggctgggatg
     1141 aaccggtgca tctcgccgaa aagatcgaga tcatcaaaac actcccgttt gtaggctttg
     1201 agactgcagc ccgaatcgtg gatcccgtcg tcgattaaaa attttcgtaa aagattggct
     1261 attcgtgaat ttaatttttt caaaaacttg tcttttctgt atttgcgcca accggaaaca
     1321 acatcatagc cttcgtccat tttttccagc aattttccga tgtccgccgg atcgttttgc
     1381 agatcgccgt ccatcgtgat cacaactttc ccggttgagt ttttgaatcc cgcgtcaaaa
     1441 gccgccgttt gcccgaaatt tttgcgctga acgatcaact tcagcggact caatgtccgg
     1501 caaatctcgt tggttttgtc cttgcttcca tcatcgatga aaacgatctc gaaagatttt
     1561 ccgagcgcct gacaagcttc gacgatcttt tggtgcaatt cccgcacatt tccctcttca
     1621 ttatagatcg gcacaacaac ggataaatcc ggtttttggt tggtgttttc catatattta
     1681 tgataatcta aaaaaggatt aaaatcaaag aaaaattcat ttatgaaaat atttttgggt
     1741 attttggtgt tttttctcgc ttttggctgg ctggttttgc tgctggcggc taccggctta
     1801 ttttacttcg gagcggtcgt tttgtcaatc gttgccagcg ccgttttggc ctggctattt
     1861 ttgcctaaga taagccaaaa tatcaacgtc aaaaccggca aaatattcta tcttgtgatc
     1921 ctgatttccc ttctcgtttc tcttgccgct tgccatttta ccaccccgac cgttttcggc
     1981 ggacgagatc aaggttcgat cggcacggcc gcaatctatc tcgcccaaaa ccacaatacc
     2041 aaagtcgatc ttccaatcgc caatgacctt tttcaaaaat acggaccggg aaaggcgctc
     2101 aacttccccg gctttgacta cgccaagaat ggcagtcttt tttcccgttt tccgctcggc
     2161 ttcacggcct atctcgcttc ggcatatgat ctgttcaacc tcaagggcat ccaatatgcc
     2221 aacttcatcc cgctattttt ctttttggtc attttttggc tgatcctcaa agaatttttt
     2281 gatgacaaag tttctctgct cggatttttc ctcgccgcgt ccttttttcc ctttctgtgg
     2341 tttgccaaat acgccctcac ggaaacatat atgctttttc tcgtttgggc gggaatattt
     2401 ttcttgcttt cgtgtagacg tccgacgtct acaaatttag acgtccgacg tctacaaatc
     2461 tctctcgcct tcttcggact ttccgcgctc gtgagaatcg agggcattgt tttctttttc
     2521 ctggcagcca tatatattct cctgctgcaa agaaaaaaaa ttctgatgct tcccaaaaac
     2581 ttcaaaagaa atctcgtgat tgcgactttg gttttgctcg gcctatacgt atatctaaat
     2641 tttcccgatc tcgtcgacag cggcaagaat ttggtcaaag cgtttctccc caactcggcc
     2701 aaagaatccg ccccgtcaac caatatgtat gagtatcttg ccagaatatt tttcaattat
     2761 aatctgctcg cctatattct cattgga
//
LOCUS       DATKGS010000046         9067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_162309, whole genome shotgun sequence.
ACCESSION   DATKGS010000046 DATKGS010000000
VERSION     DATKGS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162309"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VMQ48_01085"
     CDS             <1..596
                     /locus_tag="VMQ48_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08665.1"
                     /translation="SESETIDVRNNSGKQINLAGWKIATGSGDKIFNHPISGEISADP
                     NETKTITREFSKFSLNNKAGKVQLVMPDGKVIDEVTYSKEKITEDEAYVKMNGEWQWL
                     APNASNENPDKSNTAENNIVAPDQSNNENANNGEVLGATDENQSVSSSYRSSFNSENA
                     YIFLSRINFVSSSKQKINFCSASDPSSNIAFLIASLI"
     gene            660..1376
                     /locus_tag="VMQ48_01090"
     CDS             660..1376
                     /locus_tag="VMQ48_01090"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUD08666.1"
                     /translation="MSGHSHWAGIKHKKGVADAKRAGVFTKVARIVTLAAKEGGGNPD
                     MNFKLRIAIDQARAVNMPKDNIDRAVKRGTGELKDAAQIEEVVYEAMAPGNIAMLIKT
                     ATDNKNRTVSELKAIFNKTNVKMVPAGSFKHLFRLVGIIYVDTTDKNADELEMVAIDA
                     GALDTIFQGELLQIYTDPKELMKVKNILEKDGMEIDNANIIFHPLDPIEIDPHTKIDY
                     ENLLEKLDELDDVQEIYDNL"
     gene            1373..1867
                     /gene="ruvC"
                     /locus_tag="VMQ48_01095"
     CDS             1373..1867
                     /gene="ruvC"
                     /locus_tag="VMQ48_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HUD08667.1"
                     /translation="MKILGIDPGTATTGWAVVEEIKKNSRLVACGCVNTSKFHSDEDR
                     LVEIGKDIALLIKKYQPDEAAIEDLFFFKNLKTAITVAQARGVILYEIKKNIVPLFSY
                     TPLQVKQALTGYGRADKNQIQEMVKNILKLKSIPKPDDAADAVAIAICHLNSRITKNL
                     REKH"
     gene            1939..2583
                     /locus_tag="VMQ48_01100"
     CDS             1939..2583
                     /locus_tag="VMQ48_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HUD08668.1"
                     /translation="MNQFVSLALSNGVPLDTLVLVLILPIIVTLIAFFRQVIGIKAFG
                     IYTPAIITFAFLATNQIKYGIIIFITVIAMGTFVRYLLKQFRLLYLPRVAIMITIVGF
                     AILALLIIGGIYNRTGLASVSIFPILIMITLVEKFVAVQIEKGGRTAVILATETLIIS
                     VIGYYIASWPALIGFVLNYPWISLFTIPFNIFMGKWTGLRISEYFRFRQIIKSK"
     gene            2580..3986
                     /locus_tag="VMQ48_01105"
     CDS             2580..3986
                     /locus_tag="VMQ48_01105"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HUD08669.1"
                     /translation="MIAFFKNSRKILGMNARNLQFIRPNNLRRAKRLADDKLLSKKVL
                     KKADLPVPNLIAKIKSADELENFDWNILPESFVLKPNRGFGGEGILIVYGKKKNIENT
                     WIKADGSLITVNDIKTHITNIVDGSYSLSGVPDIAFFEERLKLLKLFKPYAFKGIPDI
                     RIIVYNKVPMMAMLRLPTRESEGKANLQQGGVGVGIDMASGVTTTAVYHKNTIIEYLP
                     GTRLVLSGIRIPYWKNILGLAVRAQEISGLGFLGADIAIDRDHGPVFLELNARPGLSI
                     QIANLDGLLGRLQRVEGLKIKTAEKGVAVGMNLFGGEIEEEIEDISGKKMLGTVEKVK
                     LTGKNEKEVEVEARIDTGAGISSIDKKLARELGFEEAVKYWEDFHLDRVLSDEEIKEL
                     SANETWKKLEQHPDIVGVAKTFSSHGATYRIEIKINLVLNDTLVVSTISIANRSGMKY
                     PILIGRRDLKRFLINPAQ"
     gene            3989..4942
                     /locus_tag="VMQ48_01110"
     CDS             3989..4942
                     /locus_tag="VMQ48_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUD08670.1"
                     /translation="MIKNPPIIKRVVREMGGKIEELIPRRGYFYIVLKGKKFLVSRKF
                     KIASNLITGGETTKFKDLTYLLLNNKGIPTPKTISIHRKLLPNELHARLKTLKFPIIV
                     KDAQGSQSKGIFANIADIKTAEKIIKREIKNFPRLIAQEMVKGDEYRITVLGNKLLGA
                     VKLIPPRIIGNGKDSVRKLIEQKQKNARRKTPFDSSLNNTLAEQGFSLRSIPKKGTIV
                     YIRRNSVLDEGGETEDATDQVHKKIKAMCTAAAMATGKYLAGIDVMCDDISKDPRKQN
                     FNIIEINGKPDIYLHYNPTRGKTRNVVKDIVTFILKLKNIG"
     gene            5545..7731
                     /locus_tag="VMQ48_01115"
     CDS             5545..7731
                     /locus_tag="VMQ48_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HUD08671.1"
                     /translation="MKKPKKRWFWRLLKLASWGLVAFLLLAIGVFAYYAKDLPSPTNL
                     NTRQVVESTKIYDRTGEHMLYEIHGEEKRTSIPFDQMSAILRAATISAEDQSFYQNPG
                     VDFKAVARAAIYDVLGRSASQGGSTITQQLIKNTVLTNEKTFTRKVKEVILSVELEQK
                     FSKDQILEMYLNQIPYGSNAYGIQAASQTFFGKDAKDLSLAESALLAGLPKAPTYYSP
                     YGSNQDELKYRQEYILDQMTKLGYITTDQAKEAKAVDVIAEIRPFQENISAPHFVMYV
                     KDQLVNQYGEKQVEEGGMKVYTTLDWDKQQAAETAVKNGVATNVAKYKATNAALVAID
                     PKTGQILAMVGSRDYFDKTIDGNVNVAIRPRQPGSSFKPYVYATAFKKGYTPDTILFD
                     AETNFGTKDQPYIPEDYDGKFRGPVKMKEALAESLNIPAVKTLYLAGVNNAINTAKSM
                     GITTINDPSRVGLSLVLGGGEVTLLDHVNAFGTFATAGIRHDKTAILKIEDSQGKTLQ
                     EYQSSPGERVLDTNVCAQIDSILDDNKLRAPIFGSNTPLRFDDRQVAAKTGTTNEWRD
                     AWTVGYTPGIVAGVWAGNNDNSPMAQGADGVFSAAPIWRDFMNHALANSAIEKFPDAT
                     VDKTGKPVLDGDVNTDKQDSLVCKMKKNKYCIPSGNSCPNGTDEKQKKISNAHNILYW
                     VNKDDPRGAIPKNPQQDPQFNAWESGVQAWAAKNGDKSGNSYDSCN"
     gene            complement(7735..8028)
                     /locus_tag="VMQ48_01120"
     CDS             complement(7735..8028)
                     /locus_tag="VMQ48_01120"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HUD08672.1"
                     /translation="MKNIQDILKRKAGREARGEKKELDEKTIGRVFLEAAKGEIKNIE
                     DGDIREMKIKEKTLYVKTAHPVVSSELFLRKEKILEKTNEIAGGKVIEKVIIG"
     gene            complement(8030..8434)
                     /locus_tag="VMQ48_01125"
     CDS             complement(8030..8434)
                     /locus_tag="VMQ48_01125"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HUD08673.1"
                     /translation="MKIEWKDEYSVGIKEIDDQHKYFISLLNDLYNAIMAGKSRKELK
                     DLFSYLADYAEKHFATEEKYFDEFNYEGAAEHKLKHQEMRDEIEKIKNMDDGKEIDFY
                     GNIVYFLKNWLEEHLAEMDQKYEKCFAEHGLK"
     gene            complement(8469..8777)
                     /locus_tag="VMQ48_01130"
     CDS             complement(8469..8777)
                     /locus_tag="VMQ48_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08674.1"
                     /translation="MPKKKTNKWNLKKVIYVLLAVVLGKLLGFIAFGLLSIEFIKMLT
                     RRGLPVEYDQIFWFVYSPLPAYLYWTLIYAGVIGGFFLGMRWWQMVYVEHRHWKKWGK
                     "
     gene            complement(8787..9044)
                     /locus_tag="VMQ48_01135"
     CDS             complement(8787..9044)
                     /locus_tag="VMQ48_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HUD08675.1"
                     /translation="MYYLYILECADKTLYTGITTDLERRMVEHGGTKLGSKYTSARRP
                     VKLIYSKKFKNRSAASKAEARIKKLKKNEKLVLIGKYKNNK"
BASE COUNT         2732 a         2167 c         1913 g         2255 t
ORIGIN      
        1 attccgaaag cgaaacaatc gacgttcgaa acaattccgg aaaacaaatc aatctcgccg
       61 gctggaaaat cgcgactgga tcaggcgata aaattttcaa tcatccgatc agcggggaaa
      121 tcagtgctga tccaaatgaa accaaaacca tcacccgtga gttttccaaa ttttctctca
      181 acaacaaagc gggaaaagtc cagctcgtta tgcccgacgg aaaagtgatc gacgaagtca
      241 catattcgaa agaaaaaatc accgaggacg aggcttatgt gaaaatgaat ggtgaatggc
      301 aatggcttgc gccgaacgca agtaatgaaa atccagacaa atcaaacaca gcagaaaata
      361 atattgttgc ccccgatcaa agcaataatg agaatgccaa taacggcgag gtgcttggcg
      421 caacggatga aaatcaatca gtttcctcaa gctacagatc ttcatttaat tccgaaaacg
      481 cctatatttt cttgagccga atcaactttg tcagctcctc caagcaaaaa ataaatttct
      541 gctccgccag cgatccttcc tccaacatcg ccttcctcat cgcttcgttg atttagtaaa
      601 aaacttgaaa tatccccctt ttccgactag tattagaaag tactaatatt tttatatcta
      661 tgtcgggaca ttcacattgg gcggggataa aacacaaaaa aggagtagcc gacgcgaaac
      721 gcgccggagt tttcacgaaa gtcgcccgga tcgtgacgct ggccgccaaa gaaggcggcg
      781 gcaatcccga tatgaacttc aaattgcgca ttgccattga tcaagcacgg gccgtgaata
      841 tgcccaaaga caatattgac cgcgccgtga aacgcggcac gggcgaactc aaagacgcgg
      901 ctcaaatcga agaagtagtc tatgaagcga tggcgccggg caacattgct atgctcatca
      961 aaaccgccac cgacaacaaa aaccgcaccg tttccgaact caaagctatt ttcaataaaa
     1021 cgaacgtcaa aatggttccg gccggcagtt tcaaacatct tttcaggctt gttggaatta
     1081 tttatgtcga cacaactgat aaaaacgcag acgaactcga gatggtggcc attgacgccg
     1141 gtgcacttga tactattttt cagggagaac tgctccaaat ttataccgac ccgaaagaac
     1201 tgatgaaagt aaaaaatatt ctggaaaaag atggtatgga aattgataac gccaatatca
     1261 tcttccatcc tctcgatccg atcgaaatcg acccgcatac caaaatcgat tacgaaaatc
     1321 ttttggaaaa actcgacgag cttgatgatg tgcaggaaat atatgacaat ttatgaaaat
     1381 ccttggaatc gacccaggaa cagccacgac tggctgggcg gtggttgaag aaatcaaaaa
     1441 aaattcgcgg ctcgtcgctt gcgggtgcgt gaatacttcg aaattccatt ccgatgaaga
     1501 tcggctggtt gaaattggaa aagatatcgc cttactcatc aaaaaatacc agcccgatga
     1561 agcggccatc gaggatttgt tttttttcaa aaatctcaag actgcaatca cggtcgcgca
     1621 agcccgtggg gtgatcctct atgaaatcaa gaaaaatatc gttcccctat tctcatatac
     1681 ccctcttcag gtgaagcagg ctctgaccgg atatggccgg gcggacaaaa atcagatcca
     1741 ggaaatggtg aaaaatatct tgaaattaaa atctattccc aagcccgatg acgccgctga
     1801 tgcagtggcc atcgcgatct gccacctgaa ctcgcgaatc acaaaaaatt tgcgcgaaaa
     1861 acattgacaa accagtgaaa aatgagtaag ataaagccat taagaggggc tttttttatt
     1921 cataatcaaa ctattcccat gaaccaattc gtttcactcg ccctttccaa tggcgtgccg
     1981 ctcgatacgc ttgttttggt gctgattttg ccgatcatcg tgacactgat cgcctttttc
     2041 cgccaagtga tcgggatcaa agctttcgga atctatacgc ccgcgataat caccttcgcc
     2101 ttcctcgcaa caaaccagat caaatacggg atcatcatct ttatcaccgt catcgcgatg
     2161 ggaacttttg tcagatatct tctcaagcaa ttccgattgc tctatctccc ccgcgtcgcc
     2221 atcatgatta caatcgttgg ctttgcgatc ctcgctcttc tcatcatcgg cggaatatac
     2281 aaccgcacgg gattggcaag cgtcagcatc ttcccgatcc tcatcatgat cacactcgtt
     2341 gaaaaattcg tcgcggtcca aatcgaaaaa ggcggccgca ccgctgtcat tctcgccact
     2401 gaaactctca tcatctccgt catcggatac tacatcgcca gctggccggc gctcatcggt
     2461 tttgttttga attatccgtg gattagtctt ttcactattc cgttcaacat cttcatgggc
     2521 aaatggacag gtctgcgtat ctctgaatat ttccgctttc gtcaaattat caaatccaaa
     2581 tgatagcgtt cttcaaaaat agccgaaaaa ttctcgggat gaacgcccgc aacctgcagt
     2641 tcatccgccc caataatctc cgacgcgcca aacgtctcgc cgacgacaaa cttttgagca
     2701 aaaaagttct caaaaaagcg gatttgcccg tcccgaatct gattgcgaaa ataaagagcg
     2761 cggacgagct cgaaaatttt gattggaata ttttgccgga aagttttgtt ttgaagccga
     2821 atcgaggatt cggcggcgaa ggaattttga ttgtttatgg gaagaaaaag aacattgaaa
     2881 acacatggat caaggccgac ggatcgctca tcacggtcaa tgacatcaag acgcacatca
     2941 caaacatcgt tgacgggagc tattcccttt ccggcgtgcc tgacatcgct tttttcgaag
     3001 agcgactgaa acttttgaaa cttttcaagc cctatgcgtt caagggaata cccgatattc
     3061 gaatcattgt atacaacaag gtgccgatga tggcgatgct gcggcttccg acgcgcgaat
     3121 cggaaggaaa agccaatctt cagcaaggcg gagtcggcgt gggaattgat atggcgtcgg
     3181 gcgtgacgac cacggctgtc tatcacaaaa acacaatcat tgagtatctg cccggcacac
     3241 gtctcgttct ttccggaatc agaatcccct attggaaaaa tattttgggt ctggccgtcc
     3301 gcgcgcagga aatttcggga cttggatttt tgggcgccga cattgcgatc gaccgtgacc
     3361 acggtccagt cttcttggaa ctcaatgctc gcccgggact ttccatccag atcgcgaatc
     3421 tcgatggact actgggaaga ctccagcgag ttgaagggct caagataaaa accgccgaaa
     3481 aaggcgtcgc cgtcggaatg aatctctttg gaggggaaat cgaagaagaa attgaagata
     3541 tttcgggaaa gaaaatgctt ggaaccgtcg agaaagtgaa gcttaccggt aaaaatgaaa
     3601 aagaagttga agttgaagcg cggatcgaca ccggggctgg tatttcttcg atagacaaga
     3661 aactcgctag agagctgggc tttgaagaag cagtaaagta ttgggaggat tttcatttgg
     3721 atcgagtttt gagtgatgag gagataaaag agttgagcgc caacgaaact tggaaaaaac
     3781 ttgagcagca ccccgatatt gtcggggtag ccaaaacatt ctcttcacat ggggcaactt
     3841 atcgaattga aataaaaatt aatttagttc tcaacgatac gcttgtcgtt tcaacgattt
     3901 ccattgctaa tcgatccggc atgaaatatc caattttgat aggacggcgc gatctcaaaa
     3961 gatttttaat taatcccgca caataaatat gataaaaaat ccaccgatta taaaaagagt
     4021 tgttcgagaa atgggaggaa agatagaaga gcttatcccc agaagaggtt acttctatat
     4081 tgtcctgaag gggaagaaat ttcttgtttc acggaaattc aagattgcca gcaatttgat
     4141 aaccggcggg gaaaccacca aattcaaaga tctcacctac ctcttgctta acaataaagg
     4201 tatcccaacg ccaaaaacta tatctattca tagaaagctt cttccaaatg aattgcatgc
     4261 aaggctcaag acgctgaaat tccccataat cgtcaaagat gcccaaggat cacaaagcaa
     4321 aggaatattt gccaatattg ccgacataaa aacagctgaa aaaataataa aaagagagat
     4381 aaaaaatttt cccagactga tcgcgcagga aatggtaaag ggtgatgaat atcgcatcac
     4441 tgtcctaggg aataaattgc ttggtgcggt caagctgatt cctccccgca tcattggtaa
     4501 tggaaaggac agtgttagaa aactgatcga gcaaaaacaa aaaaacgctc gaagaaaaac
     4561 cccctttgac tcttcactta acaacacact agctgaacaa ggattttcac tccgcagtat
     4621 tccgaaaaaa gggactattg tttatattcg cagaaattcg gttctcgacg aagggggcga
     4681 aacggaagac gcgactgatc aagttcacaa aaaaataaag gctatgtgca cagccgctgc
     4741 gatggcgacc ggaaaatatt tggccggcat tgatgtgatg tgcgatgaca tatcaaaaga
     4801 tccgcgaaaa caaaatttca atattattga gatcaatgga aaacccgata tctatcttca
     4861 ttataatcca acccgcggca agacccgcaa cgtcgtaaag gatatcgtaa cttttattct
     4921 caagctaaaa aatatagggt agttggacat agatatatga atcgcaagtg cagcaaaatg
     4981 atcttattgg cccatccgtc acaatcagat cgaaccgcga ttcttgattt tcataaccga
     5041 aattttccga attcaaaatg gagcgaacaa tccttcaaaa aatatttttc cgaaacctcc
     5101 catcatcctg tttgcgtagc aataaaagac ggaaaaatct ttaacggaat tgccataggg
     5161 agattttcca caaatagccg gactagccta aatttggcca ctcttttggt ttcgaagcag
     5221 tgccgaggaa aaggctatgg ggatatcttg atgagagaat ttttcaaatc agctgcggat
     5281 atacctgatt tgaaaaaaat ccacctccat tttcgagatt ctaacggtca agtaaaaaat
     5341 ttttacagac gttttggttt taaaaagatt gaaatcaaag agagatattc caatggagag
     5401 agaaaacact atatggaaat caaccgaaaa tctattcaga cgtgcttaaa acaagattta
     5461 tgctaaaata ggcaaagaaa agaataatcc ttaatttcgt actaaagcca gatgaaaact
     5521 cttgttgata ataccaaaaa tgccttgaaa aagccaaaaa aacgttggtt ttggcggctt
     5581 ttgaaattgg ccagttgggg cttggtcgcc tttttgctgc ttgcaatcgg agtctttgcc
     5641 tactacgcca aggatctgcc gagcccgaca aacctgaaca cccggcaagt cgttgaatcg
     5701 acaaagatat atgaccgcac gggagaacat atgctctatg aaattcacgg cgaggaaaag
     5761 agaacaagca ttccctttga ccaaatgagc gcgattctgc gcgcggcaac tatctccgcc
     5821 gaagaccaga gcttttatca gaatcccggc gtggatttca aagccgtcgc ccgagcggcg
     5881 atctatgacg tcctgggcag aagcgccagc caaggaggat cgaccatcac ccaacagctc
     5941 atcaagaata ccgtgctgac gaatgaaaaa accttcaccc gcaaagtgaa agaagtgatt
     6001 ttgtccgtcg aactcgaaca gaaattttca aaagaccaaa ttctcgaaat gtatctcaac
     6061 caaattccct acggatcgaa cgcctacggt atccaggcag cctcccaaac tttttttgga
     6121 aaagacgcaa aggatttatc tctagccgag tcagcgctct tggctggtct cccgaaagcc
     6181 ccgacctact attcaccgta tggatcgaat caggatgaac tcaaataccg gcaggaatat
     6241 attcttgacc agatgacaaa actcggctac ataaccactg accaagccaa agaagccaag
     6301 gccgtcgacg tcatcgccga gatccgaccc ttccaggaaa atatttcagc gccgcacttc
     6361 gtgatgtatg tgaaagacca gctggtcaac cagtatggcg aaaaacaagt tgaagaaggc
     6421 ggaatgaaag tctatacaac cctggattgg gacaaacaac aggccgcgga gacagccgtg
     6481 aagaacggcg ttgccacaaa cgtcgcaaaa tacaaagcta caaacgccgc tcttgttgcc
     6541 atcgatccga aaacgggtca gattctcgcc atggtcggct cgcgcgacta ttttgacaaa
     6601 acgatcgacg gcaacgtgaa tgtcgccatc cgtccccgcc agccgggatc ttccttcaag
     6661 ccgtatgtct atgccaccgc tttcaaaaag ggctataccc ccgacactat tcttttcgat
     6721 gcggaaacaa attttgggac caaagaccag ccctatattc ctgaagatta tgacgggaaa
     6781 ttccgcggtc cggtgaaaat gaaagaagcc ctcgccgaat cactgaatat tcccgccgtg
     6841 aaaactttgt atctggccgg cgtgaacaac gccattaata ccgccaaatc catgggcatc
     6901 accaccatca acgatccgtc gcgtgtcggc ctttctcttg ttttgggagg aggagaagtg
     6961 acccttctcg accacgtgaa cgcttttgga acctttgcga ccgccggcat ccgtcacgac
     7021 aaaacagcca tcctcaagat tgaagattct cagggaaaaa ccctgcagga atatcaaagt
     7081 tcacccggag aaagagtgct cgacacaaac gtctgcgccc aaattgattc gatccttgac
     7141 gacaacaagc tgcgtgcgcc aatcttcgga tccaacacgc ctctccgttt tgacgatcgg
     7201 caggttgccg caaaaaccgg aaccaccaac gaatggcggg acgcctggac ggttggctat
     7261 actcccggga tcgttgccgg tgtctgggcg ggaaacaacg acaactctcc gatggctcaa
     7321 ggcgcggatg gcgtattttc agccgctcca atttggcggg atttcatgaa tcacgccctc
     7381 gccaactccg ctattgaaaa atttccggac gcaacagtcg acaagactgg aaaaccagtc
     7441 ctcgacggtg atgtgaacac tgacaaacaa gattctttgg tttgcaagat gaagaaaaat
     7501 aaatattgca ttccttccgg aaacagttgt ccgaacggaa cggacgaaaa acaaaagaaa
     7561 atctccaacg cgcacaatat tctttattgg gtgaacaagg acgatccgcg cggcgctatt
     7621 ccaaaaaacc cgcagcaaga tccccaattc aacgcctggg aatcgggcgt tcaggcgtgg
     7681 gctgccaaaa atggtgacaa gtccggaaac agctatgact cgtgcaacta ggcatcagcc
     7741 gatgatcact ttctcgatca cttttccgcc cgcaatttcg ttcgtttttt ccaaaatttt
     7801 ctcttttcgc aagaacagct cgctcgaaac caccgggtga gctgttttga cataaagagt
     7861 cttctctttt attttcattt cgcggatatc tccatcttca atatttttga tctctccttt
     7921 ggccgcttcc aaaaaaaccc ggccgatcgt tttttcatcg agttcttttt tttctccccg
     7981 cgcttcacgc cccgcttttc ttttgagaat atcctgaata tttttcatac tatttcaatc
     8041 catgctccgc aaagcatttt tcatactttt ggtccatttc tgccaaatgc tcctcaagcc
     8101 agtttttcaa aaaataaaca atgttcccat aaaaatcgat ttcctttccg tcatccatat
     8161 tctttatctt ttcaatttcg tctcgcatct cctgatgttt cagcttgtgt tccgccgctc
     8221 cttcatagtt aaactcatcg aaatattttt cttccgtggc aaaatgtttt tccgcatagt
     8281 ccgcgagata tgagaaaaga tctttcagct ctttccggct tttgccagcc atgatggcgt
     8341 tgtacagatc gttcaaaaga gaaatgaaat atttgtgctg gtcatcgatt tccttgatgc
     8401 ccacgctgta ctcatccttc cattctattt tcatgaaatt tgataaaaaa tttatggctt
     8461 tgacgttttc atttccccca cttcttccaa tgccgatgct cgacatagac catctgccac
     8521 caacgcatcc ccaggaaaaa tcctccgatc accccggcgt aaatgagagt ccagtataaa
     8581 taggccggga gggggctata gacgaaccag aaaatttgat cgtattccac cggcagcccg
     8641 cgtctcgtca gcatttttat aaactcaatt gaaagcaacc caaaggcgat aaagcccagc
     8701 aattttccga gaacaaccgc caaaaggaca tagatcactt ttttgaggtt ccacttgttt
     8761 gtctttttct ttggcatata atttttttat ttattatttt tatattttcc gatcaacacc
     8821 aacttttcat tttttttcaa ttttttgatc cgggcttcgg ctttggaggc cgccgaccga
     8881 tttttgaatt ttttcgaata tataagtttg actggccggc gagcggaagt atatttcgag
     8941 ccaagcttgg tgcccccgtg ctcgaccatt ctgcgctcaa gatctgtggt gatcccagtg
     9001 tacaaggttt tatcagcgca ttcaaggata tagagataat acatctgtta aaattatatt
     9061 tttcccc
//
LOCUS       DATKGS010000047         4006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_206685, whole genome shotgun sequence.
ACCESSION   DATKGS010000047 DATKGS010000000
VERSION     DATKGS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206685"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VMQ48_01140"
     CDS             <1..1060
                     /locus_tag="VMQ48_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUD08676.1"
                     /translation="KIELQNYKKIVDRKLAEYFEKGIKEAKKIDPLAVQAAKNIRDLT
                     LAGGKRVRAAFMYWGYKAAGGRDDDKMVEASMSIELTNIFLLIHDDIIDRDDFRHGVK
                     TIHRRYEEVARRFYKKTDPVHFGDSMALVIGDMAAAAGNEIIFNSKFPPERILKALAK
                     LQDIVSVTVGGELMDVVLEARRKATEEEILKVHENKTAKYTVEGPLHLGGLLAGADKK
                     MLDAFSAYAVPVGIGFQIQDDILGIFGDEKKLGKPVGSDLRKGVQTLLITKALENANT
                     QQRKWIQNMMGNPKADARDIEEFKKIVIDTGSLEYSKNFSQKLIKQGKDAIEKSNIPK
                     NAKEFLSGIADYLMNREV"
     gene            1068..1874
                     /locus_tag="VMQ48_01145"
     CDS             1068..1874
                     /locus_tag="VMQ48_01145"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HUD08677.1"
                     /translation="MKPLYILKIGGSVATYKNRPGFSVRSSLLKKIAISIREAQKKEN
                     FDLILIHGAGAAGHQLAKQYGLQRGTGKNQKKMHGAFLSRFANQKLDIAVAESLYEGG
                     LKIVSVHTASIIIQKNGKITKCDLKTIRDALDKECIPLLYGEMAFDEELGMTICSGDA
                     IAPYLAKELYAKKIFFASDAKGIFDKDPHIFKNVRLIEKISIKDIEKNVELSNSHNVD
                     ATDGMRGKIKNIKNLIKKSVKSVEIFDGHKVQNYKKVFLSEKFPHTVIQK"
     gene            complement(1897..2355)
                     /locus_tag="VMQ48_01150"
     CDS             complement(1897..2355)
                     /locus_tag="VMQ48_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08678.1"
                     /translation="MIIGVGFLLFSIVDGIPKLFVLRELKSKPEIYKKEGMLSFPLET
                     YQEIDGNYTNTIIRLLKEEIGISLEQASIGKIISKKFSLIPGRSDIVTVYGYGFFHGN
                     PAAYLCPSDDDITFAGWYTCEDLLNYPLIRVETAPILRHFLKKHAREILG"
     gene            2522..3235
                     /gene="uppS"
                     /locus_tag="VMQ48_01155"
     CDS             2522..3235
                     /gene="uppS"
                     /locus_tag="VMQ48_01155"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HUD08679.1"
                     /translation="MPEVKNIPNHVAIIPDGNRRWAKKKGLEPWDGHEAGAENVEKVL
                     KANLDLGVKHVTFWGSSVDNIQKRPWREKRALLNVYRKYFVRLIDNEDIHKNQVQVNI
                     IGRWEEQFPDSLKNILRKCMSRTEKYGKHFLNFLLAYSGDDEMVVATNSLLEKCKGKV
                     KRITAKMIKEHLMTRDLPPVDLLVRTGGEPHNSAGFMMWDVANAQLYFSEVLWPDFDE
                     KKMREAVLDYGRRNRRFGR"
     gene            3336..3884
                     /locus_tag="VMQ48_01160"
     CDS             3336..3884
                     /locus_tag="VMQ48_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08680.1"
                     /translation="MKLKIIILSLLIIPSLVFAQDVPKKKAYFFYGDSCPHCKKVEAY
                     FQENGIYDKYDITSLEFSNPFNTRLLLKFGDVFNDPNKGGVPAVAFGDKFIVGDQPIM
                     DNFVREIDAADNANELPDPDKIASANGTSINSNPDSQASPVANSPASVTGGNKKNYFP
                     VVIIAFVILGAGALVYVNRKKS"
BASE COUNT         1284 a          739 c          961 g         1022 t
ORIGIN      
        1 gaaaattgaa cttcaaaatt acaaaaaaat agttgatcgg aaattggccg aatattttga
       61 aaaagggatc aaagaagcga aaaaaattga tcctttggcg gttcaggcgg cgaaaaatat
      121 ccgggatctc acgctcgcag gcggaaaacg cgtgcgtgcg gcttttatgt attgggggta
      181 taaggcggcc ggaggacggg atgacgacaa aatggtcgaa gcttcgatga gcatcgaact
      241 cacgaacata tttttgctga tccatgatga catcattgat cgggatgatt ttcgccatgg
      301 cgtgaaaacg atccataggc ggtatgaaga agtggccagg cgattctaca aaaaaaccga
      361 cccggttcat ttcggagatt cgatggcgct ggtcatcggc gatatggcgg cggcggccgg
      421 aaacgaaatt attttcaact caaaatttcc accggaacgg attctcaaag ctttggctaa
      481 gctgcaggac attgtttccg tgaccgtggg aggggaattg atggatgttg ttcttgaggc
      541 gcgaagaaag gcgacggaag aagaaatttt gaaagttcac gaaaacaaaa cggccaagta
      601 taccgtggag gggccgctgc atttgggagg attgctggcc ggagcggaca aaaaaatgtt
      661 ggatgctttc agcgcctatg cagttccggt tggcatcggc tttcagattc aggatgatat
      721 tcttggtatt tttggggatg aaaagaaact gggaaaaccc gttggcagtg atttgcgaaa
      781 aggcgtgcaa acgctgctga tcacgaaagc gctcgaaaac gcaaatactc agcagcgcaa
      841 gtggatccag aatatgatgg gaaacccgaa ggccgacgct cgcgatatcg aagaattcaa
      901 aaaaattgtc atcgatacgg gatcgcttga atactcgaag aatttttcgc aaaaattgat
      961 caagcaaggg aaagatgcaa ttgaaaaaag caatattccg aaaaatgcga aagaattttt
     1021 gtcgggaata gcggactatc tgatgaaccg ggaagtgtga atattttatg aaacctttat
     1081 atattctcaa gattgggggg agtgttgcga catacaaaaa tcgtcccggt ttttctgtga
     1141 ggagttcttt gctgaaaaaa atagcgattt caataagaga agctcaaaaa aaggaaaatt
     1201 ttgatttgat tttgattcac ggcgcgggtg cagccggtca tcagttggca aaacagtatg
     1261 gactacagag aggaacggga aaaaatcaaa aaaaaatgca tggagcattt ctttcccgat
     1321 ttgcaaacca aaaattggat atcgcggttg ccgaatccct ctatgaaggc gggttgaaaa
     1381 tagtatccgt acatacggcc tcaataatca tccaaaaaaa cggaaaaatc acaaaatgcg
     1441 atctaaaaac aataagagac gcattggaca aagaatgcat tcctctgctt tatggagaaa
     1501 tggcttttga tgaagaattg gggatgacta tttgttctgg cgatgcgatt gcaccttatc
     1561 ttgcaaagga actgtatgct aaaaaaatat tttttgcctc tgatgcgaaa gggattttcg
     1621 ataaagatcc gcatattttt aaaaatgtac gccttattga aaaaatatca ataaaggata
     1681 tcgaaaaaaa tgtcgaattg tccaattccc ataatgttga tgctacagat ggaatgaggg
     1741 gaaaaataaa aaatatcaaa aatttgataa aaaaatcagt aaaatcggtt gagatattcg
     1801 acgggcacaa agtacagaac tataaaaaag tttttctgag cgaaaaattt ccacatacag
     1861 tgattcaaaa atagaaacag cggccgttgc cgctgtttag ccgagaattt cccgggcatg
     1921 ttttttgaga aaatgtcgaa ggatgggggc tgtttccact cggattagtg gataattgag
     1981 cagatcttcg caagtatacc atcccgcgaa agtgatatcg tcgtcagaag gacaaagata
     2041 tgcagccgga tttccatgga aaaagccata cccgtaaact gtgacaatgt cgcttcttcc
     2101 tggaatcagg gaaaattttt tggaaattat ttttccaatc gaggcttgct caagagaaat
     2161 gccaatttcc tctttcaaga ggcggattat agtgttggtg taattgccgt ctatttcctg
     2221 gtaggtttcc aatggaaacg aaagcattcc ctctttttta tagatttctg gtttgctttt
     2281 gagctcccta agtacgaata gtttgggaat tccgtcaaca atagaaaaga gcaaaaatcc
     2341 gacgccaata atcataaggc aacctcctgg atattcgtat gattgagctg tttcaaaaaa
     2401 tatcatagaa aatcgcaagt agcaagcttg ttgtgttttt tggaactatt ttatataata
     2461 tcagtgtgat tttgaatact aacaaaaaac gggatatacc attcccagtt ttctattttg
     2521 tatgcctgaa gtaaaaaata ttcccaacca cgttgctatc attcccgacg ggaaccgccg
     2581 gtgggcgaaa aagaaaggac ttgagccgtg ggacgggcac gaagccgggg cggaaaatgt
     2641 cgagaaagtt ctgaaagcga acctggattt gggcgtgaag cacgtgacct tttggggctc
     2701 atcagtagac aatatacaaa aaagaccttg gcgggaaaaa agagcgctgc tcaatgttta
     2761 tcgcaaatat tttgtgcgct tgattgacaa tgaagatatt cacaaaaacc aagtgcaagt
     2821 gaacataatt gggcgctggg aagagcaatt tccggattct ctcaaaaata ttctccgcaa
     2881 atgcatgagt cggacggaaa aatacggaaa gcatttcctc aattttttgt tggcatacag
     2941 cggcgatgac gaaatggtcg tcgcaaccaa cagtcttttg gagaaatgca aagggaaagt
     3001 gaagcgtatt accgccaaga tgatcaaaga gcatttgatg acgcgtgatc ttccgccggt
     3061 ggatcttttg gtgcggacgg gcggagagcc gcataacagc gccggtttca tgatgtggga
     3121 cgtcgccaac gcccagctgt atttttccga ggttctttgg ccggattttg acgagaaaaa
     3181 aatgcgggaa gcggtcttgg attatggaag gagaaacagg agattcgggc gctgacgcgc
     3241 aatttccaat gatcaatgat caattttcaa acattgagaa attgaaaatt gaagaattga
     3301 ttgaaaattg aaaatttgta aattgaaaat tatttatgaa gctaaagatc atcatactca
     3361 gtctcttaat aattccatct cttgtttttg cccaggatgt cccaaagaaa aaagcctatt
     3421 tcttttatgg tgattcttgt ccgcactgca aaaaagtaga agcttatttc caggaaaatg
     3481 gaatatatga caaatacgac atcacaagcc ttgagttttc caatcctttc aatacacggc
     3541 tgcttttgaa attcggagac gtgttcaatg atccaaacaa aggcggtgtg ccggcggttg
     3601 cctttgggga caaatttatt gtcggcgacc agccgatcat ggataatttt gtccgagaaa
     3661 tcgatgcggc agacaatgcg aatgaactgc cagatccgga caaaatcgcc agcgcgaacg
     3721 gcactagcat aaattccaat ccggacagtc aggccagtcc ggtcgcaaat tcgcctgcaa
     3781 gtgtgactgg cggaaataaa aaaaattatt ttcccgtagt aatcattgcg ttcgtcatct
     3841 tgggtgcggg agcgttggtt tatgtaaatc gtaaaaaatc ataaaaaaat ggaaatgcaa
     3901 ttcacagacc aaaatttcga agcagaagtt ctcaaaagcg acaagccggt gctcgtcgat
     3961 attcgcatca agctgtttcg caagctcaac ccccggttca ctcccg
//
LOCUS       DATKGS010000048         4542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_47571, whole genome shotgun sequence.
ACCESSION   DATKGS010000048 DATKGS010000000
VERSION     DATKGS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47571"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VMQ48_01165"
     CDS             complement(<1..926)
                     /locus_tag="VMQ48_01165"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUD08681.1"
                     /translation="MLKLTDNKNNKRVGKLSTAHGVINTPFFLPIATKGAVKQLSSEE
                     LRELGAEAILGNTYHLWLRPGEKLVKKAGGLQKFMNWNGPMLTDSGGYQVFSLAGRRG
                     DKGSVKLSEKGVEFRDPSDGKKYFMTPEKSIEIQLALGSDIIMVLDECPPYPCSREYA
                     LKSLERTTRWAERCLKYFKQRMQNGKIKMQNDNLKLKNNKKIPNTKYQIQDTKRPLLF
                     GIVQGSVYKDLRKESAKQLLELGFDGYAIGGVAVGEPREKMKKILEWVLPMLPEDKSR
                     YLMGLGRPEEIVFAVNHGIDMFDCVIPTRNAR"
     gene            complement(1027..2964)
                     /locus_tag="VMQ48_01170"
     CDS             complement(1027..2964)
                     /locus_tag="VMQ48_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08682.1"
                     /translation="MSLEETERKIYQREEDPSLNLEQRRALNPHTAEEVPFAAASLET
                     QSLEQKKEVWIKEQEEKKEKRKKILKKALIVLGALALVAGIISLALYIRKSAFTEQQV
                     KVSISGPEKVKSGESVSFDIDYQNLNRASLNGAVLYINYSENFKPSGNLQFESEGPST
                     SKFNIGNIAGKTNGKVTIQGKFFGASDALVYFEVKLDYKPSTFNSTFEAKANSSVFIS
                     SSPLTIEVSGPLNEASGNAVSYTVKYQNTGQEDFNDLKLKADFPDGFSFSSSDPLAAQ
                     GNNIWYIGTLAAGQSGQVKVNGTVAGARDEQKTTKFSIGEIGSDNAFISYGEAASTVK
                     IIGSPIVLKETINGKSEDVSVSAGESVSFKIGYQNTGSIGLRDVVFSVEANSPILDYS
                     RINEQGAKGEFDGDKKIITWKSSEIPDFKMLSPGAQGEIDFSIPVKDVIPVSGPKDKN
                     FSFSAVARMDSPDIPTPEGSNKVVASNSVNVKLNSKLLAGLQGFFNDADIVNSGPIPP
                     KVGQETTFALHLKIANVSNDVTDAKVAMTLAPGVKWKNNFLPKDASMSYNDRTNELDW
                     NVGSLAAGTGVTTDPKEVIFQLGVTPTLNQVSDFAPLVSQTVFSAKDSFTNQPLELKI
                     GAKNTNLPEDLGVGDMGKVTP"
     gene            complement(3189..3896)
                     /gene="recO"
                     /locus_tag="VMQ48_01175"
     CDS             complement(3189..3896)
                     /gene="recO"
                     /locus_tag="VMQ48_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HUD08683.1"
                     /translation="MQKYTAIILSSHDSGEFDRLYILYTKEIGVVKAMGRGVRKQTAK
                     LAGHLEPGTLSEVYVARARGMGQITGAIALDNFENIKNNFEKLAEVLKIFKFFTRHFS
                     EEEKDERIFELLSEFLKEADVAERADEADILVEAFWWKLFDALGNRPETMKCAKCEAR
                     LEEKGEKYFSSSRGGIVCGRCFAGEQDLAKISSDQIKLLRIFLANPLEKILKVKVEKT
                     ELRGLGNIRREFERYNF"
     gene            3932..4318
                     /locus_tag="VMQ48_01180"
     CDS             3932..4318
                     /locus_tag="VMQ48_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08684.1"
                     /translation="MRPLIGRLISFLASPNGEPEYSYCCQQTNQRQQYLSAEETVKNK
                     KRNSISSRWQNHAYDQNQVLFSVYLRHIVLLRRISDLIMGCLKISIEKSKAERKQNEE
                     AANPLTPQLLIWNCERQNNDNDNCAD"
BASE COUNT         1110 a         1156 c          903 g         1373 t
ORIGIN      
        1 tgccgcgcgt tgcgggtcgg gatgacgcaa tcaaacatat caatgccgtg attcaccgca
       61 aaaactattt cttcgggacg gccaagcccc atcaaatatc tcgacttgtc ctctggcaac
      121 atcggcaaaa cccattccaa aattttcttc atcttttcgc gcggctcccc gaccgccacc
      181 ccgccgatgg catagccgtc gaagccaagc tccagcagct gcttcgcaga ttcttttctc
      241 aaatccttat atacactgcc ttgtacaatg ccaaacagta agggcctttt tgtatcttgt
      301 atttggtatt ttgtatttgg tattttttta ttatttttta attttaaatt gtcattttgc
      361 attttgattt ttccattttg cattcgttgt ttgaaatatt tcaaacaacg ttccgcccat
      421 ctggtagttc tctccagtga tttaagcgca tattcacgcg aacatggata aggcgggcat
      481 tcgtcgagaa ccatgataat gtcgcttccg agcgccagct ggatctcgat cgatttttcc
      541 ggtgtcatga aatatttttt tccgtcggac ggatcgcgaa actccacgcc tttttcggac
      601 agtttgacac tgcccttgtc acctcttctt cccgccaggg aaaaaacttg gtatccgccc
      661 gaatccgtca gcatcgggcc gttccaattc atgaattttt gcaaaccccc cgcctttttg
      721 acaagttttt cgccgggccg gagccaaaga tgatacgtgt tgcccagaat agcctccgcg
      781 ccgagctctc tcagttcttc cgaactcagt tgcttcaccg cccctttcgt cgcaatcggc
      841 aaaaaaaagg gagtgttgat aaccccgtgc gctgttgata attttccaac ccttttgtta
      901 tttttattat cagttaattt aagcatacat aaatttttta atttcagaat tttcaatttt
      961 caaacaatga ctgaatttct aaattttaaa attgtttgaa aattttggaa ttttgacatt
     1021 gaaaatttac ggcgtcactt tccccatatc tcccactccc aaatcttccg gtaaatttgt
     1081 attttttgcg ccgatcttca gctcaagcgg ctggttggtg aaagaatctt tggcggaaaa
     1141 taccgtttgt gaaacaagcg gagcaaaatc actgacttgg ttcagcgtcg gagtgacacc
     1201 gagttggaaa ataacttctt tcggatcagt ggtcacaccc gttccggcgg caagactgcc
     1261 tacgttccag tccaattcgt tcgtccggtc gttgtagctc attgacgcgt ccttgggaag
     1321 aaaattgttt ttccatttta cccccggcgc gagcgtcata gccactttcg catccgtcac
     1381 gtcgtttgaa acgttggcta ttttgagatg cagcgcgaaa gtcgtctcct ggccgacttt
     1441 gggggggatc ggaccggagt tcacgatatc ggcatcgttg aaaaatccct gaaggccggc
     1501 cagcaatttc gagttcaatt tcacgttgac cgaattgctt gccacaacct tgtttgatcc
     1561 ctcgggcgtg ggaatatccg gactgtccat ccgggcaacc gccgaaaagg agaaattttt
     1621 gtctttcggc ccggaaacag gaatgacatc cttcacggga atggagaaat ctatttcccc
     1681 ctgcgcgccc ggagagagca tcttgaaatc cggaatttcg gaagacttcc aagtgatgat
     1741 ttttttatcc ccgtcaaatt ctccttttgc accctgctca ttgatccgcg aatagtcaag
     1801 gatggggctg ttcgcctcaa ccgaaaaaac aacatcccga aggccgatgg agccagtatt
     1861 ttggtagccg atcttgaagg acacggattc tccggcactg accgagacat cttcactttt
     1921 gccattgatt gtctctttca aaacgatcgg cgacccgatg atcttcacgg tgctggccgc
     1981 ttctccgtat gaaatgaatg cgttgtctga cccgatttct ccaatggaaa actttgtcgt
     2041 tttttgctca tctctcgctc cggcaaccgt cccattgacc ttcacttggc ctgattgtcc
     2101 cgccgccaaa gtcccgatat accaaatatt gtttccttgg gccgccaaag ggtcagagct
     2161 tgaaaaagaa aacccgtccg gaaaatcggc tttgagcttg aggtcgttga aatcttcctg
     2221 tcccgtattt tgatatttca ctgtgtagga aacggcattt cccgacgcct cgttcagcgg
     2281 tccgctcacc tcgatcgtca gcgggcttga ggatatgaaa acgctcgaat tcgccttggc
     2341 ttcgaaagtc gaattgaatg tcgacggttt gtaatcaagt ttaacctcga aatataccag
     2401 cgcatcgctg gctccgaaaa actttccctg gatagtgacc tttccgttgg tttttcccgc
     2461 gatgttcccg atgttgaatt tgctcgtgct cggcccttcg ctttcaaatt gcaaattgcc
     2521 cgagggtttg aaattttcgg aatagtttat atacagcacg gcgccgttca ggctcgcccg
     2581 gttgaggttt tgatagtcga tgtcgaaact gacgctctca ccgcttttca ccttttccgg
     2641 tccggaaatg gaaactttca cctgctgttc ggtaaaagcg cttttgcgga tatacaaggc
     2701 cagtgagatg attcccgcca caagcgcaag cgcgcccagg acgatcaaag cttttttgag
     2761 tatttttttt ctcttttctt ttttttcttc ctgctccttt atccagacct cttttttctg
     2821 ctccaggctc tgcgtctcca aagaagccgc ggcaaaagga acctcctccg ccgtgtgggg
     2881 attcaaggcc cttctttgct ccaaattcaa acttggatct tcctctcttt ggtatatttt
     2941 tctttctgtt tcctcgagag acatcttact gaatattata cactaaatat cgctttttcg
     3001 taaacgactc ttctattaat taaaatgtct tatttatact atcaattttc aatttcaaat
     3061 tttcaatttt caaacaatgt ctaaatgact aaatgaaaaa acagaaaact atggtttaga
     3121 aaaaatataa tttaaaattt tgtcatttgg gatttcattt caaatttaaa atttagcctt
     3181 tcaaattatc agaaattata gcgttcaaac tctcttctta tattccctaa acctctcaac
     3241 tctgtttttt ccactttaac tttcaaaatt ttctcgagcg ggttggcgag gaatatccga
     3301 agaagcttga tttggtcgga ggatattttc gcaaggtcct gctcgccggc aaaacatctc
     3361 ccacaaacaa ttccgccacg agaagaggaa aaatattttt ctcctttttc ttcgaggcgc
     3421 gcttcgcact tcgcacattt catcgtttcg ggccggttcc cgagcgcatc aaacaacttc
     3481 caccaaaacg cttcgactag aatatccgct tcgtccgctc tttccgccac atctgcttct
     3541 ttcaaaaatt cactcaacaa ttcaaaaatt ctttcgtctt tttcttcttc ggaaaaatgg
     3601 cgcgtgaaaa atttgaatat tttcaaaact tcggccagtt tttcaaagtt gttctttata
     3661 ttttcaaaat tgtcgagggc aatcgcgccc gtgatctgtc ccattccgcg cgccctggcg
     3721 acatagacct cggaaagcgt ccccggctcc aagtggccgg cgagcttggc ggtctgtttt
     3781 ctgactcccc gccccatggc tttcaccacc ccgatttcct ttgtataaag gatatacagc
     3841 cggtcaaatt caccggaatc atgggaggac aaaattatgg ccgtgtattt ttgcatggtt
     3901 caattatatc ataaaaaggg ggccgcaccg tatgcgaccc ctaataggaa gattgatttc
     3961 ttttctcgcc tctccaaatg gcgaacccga gtatagctac tgctgccagc agaccaatca
     4021 gcgtcagcaa tatctttccg cggaggaaac agtcaaaaac aaaaaaagaa attccatatc
     4081 ctcccgctgg caaaatcacg catacgatca aaatcaagta ctcttttctg tttatttgcg
     4141 ccatatcgtc ctccttcgcc ggatttccga cttaataatg ggatgcttga aaatcagcat
     4201 cgaaaaaagc aaggcagaac gcaaacagaa tgaggaagcc gccaatccat tgacgcctca
     4261 actcctcatc tggaattgcg agcgccaaaa caatgataac gacaattgcg ccgactaggc
     4321 taatgatata tttcatcaga ttcccccttt tttcaaagat caatcttcgc ccaagatctc
     4381 agctcttggt tattttatat ctattttctt gagccatact ttcaccggtt cccgctcgag
     4441 cgccaccagt tctgaaatgt cggcttcagg gtcttcatga tcgcagatat cctctgcgag
     4501 tgtttctttg gcaatcagct cgccgaattt ttcaaatata tc
//
LOCUS       DATKGS010000049         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_176665, whole genome shotgun sequence.
ACCESSION   DATKGS010000049 DATKGS010000000
VERSION     DATKGS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176665"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VMQ48_01185"
     CDS             <1..483
                     /locus_tag="VMQ48_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HUD08685.1"
                     /translation="YIDEFQNFTTDSIAQILSEARKYRLALIIAHQFIAQLKEEISKA
                     VFGNVGSICSFRVGAEDAEFLQKQFEPVFDANDLMNVDNHNCFMKLLIASQVASPFNM
                     KTYPPTAGSKEIAEAIKELSKLKYGRNRDIVNEEILKKSSFVSAPASADAFDMGEKPL
                     "
     gene            507..680
                     /locus_tag="VMQ48_01190"
     CDS             507..680
                     /locus_tag="VMQ48_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUD08686.1"
                     /translation="MDQHDMQTEKSPEEETVDPHCVTCGMPLTKAEDFGTNSDGSRNE
                     QYCCHCMQGGNLF"
     gene            746..877
                     /locus_tag="VMQ48_01195"
     CDS             746..877
                     /locus_tag="VMQ48_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08687.1"
                     /translation="MNPVTPVEPMAAPEVPMETPAEPMAPEAPVMPEEKPVDMPMQE"
     gene            complement(974..1312)
                     /locus_tag="VMQ48_01200"
     CDS             complement(974..1312)
                     /locus_tag="VMQ48_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08688.1"
                     /translation="MAIKLESEPTKIDQGLFKKLYDTAILKLPDNLRRDGIKKKNPAY
                     NKTLKTEIELFLSSHGLIKTILEKPQGDAKILFDSIFNYLKVPEEILPEIRSEKNLPK
                     DRQRDLLDLA"
     gene            complement(1302..>2284)
                     /locus_tag="VMQ48_01205"
     CDS             complement(1302..>2284)
                     /locus_tag="VMQ48_01205"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HUD08689.1"
                     /translation="TVLAFDWNASGLTTILTAALKEAIRGEERDLGIYICGGKGKTSL
                     KTPEQISLFGARAELSQEKINSLEYNSRMAAKVDSSLIQDGFQIYHHCFFFSRNGAWA
                     VVQQGMNEKNVTARRYHWFSDDAKNMVIEPHAGIISDGRHTGLNMTARESENTQRIST
                     ELVQGSYNTLMKDLKLLRKYSTPQSEVISIGNENQELVKMHLHDRDFKVLPVITEDFT
                     KSKYLEKILYEINEIKPKNYEQLLSLKGVGPKTIRALALTAEVIYGAKASYEDPARYS
                     FAHGGKDFIPYPVDRPTYDQTIATMRQLASKMKIGYSEKNKIIGRLNYGN"
BASE COUNT          576 a          504 c          529 g          675 t
ORIGIN      
        1 tatatcgatg aatttcaaaa tttcacgacc gattcgatcg cccagatcct ttcggaagcg
       61 cgaaaatatc gcctggcgct catcatcgcc catcaattca ttgcccaact caaagaggaa
      121 atttcgaaag ccgtgtttgg aaacgtggga tcgatttgct cgtttcgggt gggagcggaa
      181 gatgcggaat ttctgcaaaa acagtttgag ccggttttcg acgcgaatga tctcatgaac
      241 gtggacaatc acaactgttt catgaagctt ttgatcgcga gccaggtcgc ttcgcctttc
      301 aatatgaaga cctatccgcc gacggccgga tcgaaagaaa ttgccgaagc catcaaagag
      361 ctttccaaat tgaagtatgg gcgcaatcgt gatatagtga atgaagagat cttgaaaaaa
      421 tcaagttttg tttccgctcc cgcttcggcc gacgcttttg atatggggga aaaacccttg
      481 taaagataat tttttatatt gccattatgg atcaacatga catgcagaca gaaaagtcac
      541 cggaagagga gacagttgac ccgcactgcg tgacttgcgg tatgccgctc acgaaagcgg
      601 aggatttcgg gaccaattct gacggtagcc gcaatgagca gtattgctgc cactgcatgc
      661 aaggcggaaa tttattttaa gtgatagata atattaagta tattactcta aaaactagaa
      721 agagaggtgt aacatgactg acgatatgaa cccagtgact ccggttgagc cgatggcagc
      781 tccagaagtt ccgatggaaa ctccagctga accgatggct ccggaagctc ccgttatgcc
      841 ggaagagaag ccggtagata tgccaatgca agagtaattt tcgtaaattg ctcatcaaaa
      901 aagccagcca agagggaccg gcttttttgt tttatcgtgg aataaaaaac cgcccagaat
      961 tgtctaggcg gggtcaggcc agatcaagga gatctctttg ccgatctttt ggcaaatttt
     1021 tttcactcct aatttctggt agaatttctt caggaacttt taaatagttg aagatgctgt
     1081 cgaataatat ctttgcatct ccctgaggct tttccagaat tgtttttata aggccgtggc
     1141 ttgacaaaaa gagttcgatt tctgttttca gggttttatt atacgcaggg tttttctttt
     1201 ttattccgtc gcgcctcaag ttatccggta gctttaaaat tgctgtatca tataacttct
     1261 tgaagagccc ctggtcaatt tttgttggtt cactttcaag cttaattgcc ataattcaac
     1321 ctcccgatta tcttattttt ctcactatat ccaattttca tcttggatgc aagctggcgc
     1381 atggtcgcga tcgtctggtc ataggtcggc cggtcgacgg gataggggat gaagtctttg
     1441 ccgccgtggg cgaaggagta gcgggccggg tcttcatagg aagctttcgc gccatagatc
     1501 acttccgcgg tcagcgccaa ggctcgaatc gttttcggcc cgactccttt gagtgacaaa
     1561 agctgttcat aatttttcgg cttgatttca tttatttcgt ataaaatttt ttccaaatac
     1621 ttgcttttgg taaaatcttc cgtgatgacg ggcaaaactt tgaaatcgcg gtcatgcaaa
     1681 tgcattttca cgagttcttg attttcgttt ccaattgaaa tcacttcact ttgtggtgtc
     1741 gaatattttc ggagcagttt caaatctttc atcagcgtgt tgtagcttcc ttgaacgagc
     1801 tccgtgctaa tcctttgcgt gttctcactt tcacgcgcgg tcatgttcag tccggtatgc
     1861 cggccatctg aaattattcc ggcatgcggt tcgatgacca tatttttggc gtcgtcggaa
     1921 aaccagtgat aacgccgtgc tgtcacattt ttttcgttca ttccttgttg gacaaccgcc
     1981 caggcgccgt ttcgggaaaa aaagaaacaa tgatgatata tttgaaaccc atcttgaatg
     2041 agtgaggaat cgactttcgc cgccatgcga gaattgtatt ccagggaatt tattttttct
     2101 tgcgaaagtt cggcccgcgc gccaaagagg gaaatttgct ccggagtttt gagggaagtc
     2161 tttcccttcc cgccacaaat atatattccc aaatcccttt cttctccgcg gatcgcttct
     2221 ttgagcgccg ccgtgagaat cgtcgtcagt cctgatgcgt tccaatcgaa agcgagtacg
     2281 gttc
//
LOCUS       DATKGS010000050         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_162714, whole genome shotgun sequence.
ACCESSION   DATKGS010000050 DATKGS010000000
VERSION     DATKGS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162714"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1085)
                     /locus_tag="VMQ48_01210"
     CDS             complement(108..1085)
                     /locus_tag="VMQ48_01210"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HUD08690.1"
                     /translation="MKYKFAEEFNQIKKEFEKSRKILLVAHSRPDEDTAGSVFVLKGY
                     LKNNLGKIVDVVCLDPLPEYLAVIFEGEKFLKSQEIRIEDYDLIVGCDAVERSFEKIV
                     NKKNENQKIILFDHHPTLELKEIKPDIVVTDVKYSSVCEIIYDFFDFHRIALNRKMAS
                     YLLAGILYDTGAFQHANTSVRVMEISADLMRKGAPMNKIIEATFKNKRLETLKLWGRA
                     MQRAEINEKTGAILSFITREDMLELGIKEDEIGRIAELLNTVVGTKFSLVLIEKGGNK
                     IKGSLRSEWYKNVDVSEIAQKLRGGGHRLASGFEIVGKIVKNEEGISVE"
     gene            complement(1082..1411)
                     /gene="rbfA"
                     /locus_tag="VMQ48_01215"
     CDS             complement(1082..1411)
                     /gene="rbfA"
                     /locus_tag="VMQ48_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HUD08691.1"
                     /translation="MPLRLNRVNELIRTTVAEIIAREVELPLGSFVTVVKVDTTRDLR
                     YARVFVSVFPEKRFGKTLEFLRKRLYLIQGALNKKLHMKPLPRLEFVADKTEAEADKI
                     EKLLKEL"
     gene            complement(1417..>3187)
                     /gene="infB"
                     /locus_tag="VMQ48_01220"
     CDS             complement(1417..>3187)
                     /gene="infB"
                     /locus_tag="VMQ48_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HUD08692.1"
                     /translation="KVKMPSRVTVKKFAEVLGLGISDVIKELMKNKILATINDEIDFE
                     TAAIIASDLGFEAEEDIEAGGQAAMTLEKLDEILKKEKDASSTEVGLRHRAPVVTILG
                     HVDHGKTTLLDTIRKTSVVEKEAGGITQHISAYQVKKKGEPITFVDTPGHEAFSAMRE
                     RGVSLADIAILVVAADDGVRPQTEEVINYLKEKKIPMVVAINKVDKPNANVQRVKQEL
                     ADRGIVIEDWGGDVVAAEISAKQNIGIDELLDMILLVAEVENLKADYKRLPLGVVIES
                     HLDPKKGNMSTVLVRTGTLKEGQDILAGSIAGRVRRLEDFKGKQVKEAGPSTPVTVIG
                     LHELPNTNDVLQGAVDLKSAKMSQKEMQSGGFGGMKKFDAQTLNKSIEDQNIKKINII
                     LKTDVQGSLEAVDQILGAIKSDEVGINYIRQAVGDITESDVKLAQTSGAVIFGFNIQP
                     TTVAKRLAEMNKVEIKSYKVIYELVDDIKKRLEAMLGENIVRTELGKLKVLAVFMTGK
                     KDMIVGGEVISGKMKNGELLEVTRADIVLGRGKLINLQQNKVDVDEVTQGKECGITFD
                     GETKIKEGDILLCFHEEVVKKTL"
BASE COUNT          746 a          857 c          564 g         1020 t
ORIGIN      
        1 tttccctcgc ctggaacaac ctggctgcaa gtcccacgca agaggcatga gcaacatttt
       61 tttatatttt cttcgaggat cttgtattgc ataagagtaa ttgaaaatta ctcaactgat
      121 atcccttctt catttttcac aattttccca acaatttcaa atccgctcgc gagccggtgt
      181 ccgccgcctc gcagcttctg ggcaatttcg gaaacgtcca cgtttttata ccactcactc
      241 cgcagacttc ctttgatctt attccctcct ttttcgataa gaaccaagga aaactttgtt
      301 ccaacaactg tgttcaaaag ttcggcgatc ctgccgattt catcttcttt tatgccaagc
      361 tccagcatat cttccctagt aatgaaagac aaaatagctc ccgttttttc attgatttcc
      421 gcccgctgca tcgcccgtcc ccatagtttc aatgtttcca atcttttgtt tttgaaagtc
      481 gcttcgatta ttttattcat cggcgcccct tttctcataa gatccgccga aatttccatt
      541 acccgcacag aagtattcgc gtgctgaaac gcccctgtat catacaaaat tccggccagc
      601 agatatgacg ccatttttct gtttagagca attctatgaa aatcaaaaaa atcataaatt
      661 atttcgcaga ctgatgaata ctttacatcg gtcactacga tatccggttt tatttcctta
      721 agttcaagtg ttggatgatg atcgaaaaga attatttttt gattttcatt ttttttgttt
      781 actatttttt caaaacttct ctccaccgca tcacatccga caatcaaatc ataatcttct
      841 attctaattt cttgtgattt taaaaatttt tcgccctcaa aaattactgc gagatattcc
      901 ggcaatggat caagacagac tacatcaaca attttaccta gattgttttt tagatatccc
      961 ttcaaaacaa aaacagatcc cgctgtatcc tcgtccggcc gcgaatgggc aaccaggaga
     1021 atttttcggg acttttcaaa ttcttttttt atttggttga attcttcagc aaatttatac
     1081 ttcatagctc tttgagtagt ttttcgatct tatccgcttc cgcttcggtt ttgtctgcga
     1141 caaattccaa tctcgggagc ggcttcatat ggagtttttt gttcagagct ccctggatca
     1201 gatacagtct tttccgcaaa aattccaatg tcttgccgaa cctcttttcc gggaatacac
     1261 tcacaaacac tcgagcatag cgcagatccc gagtcgtgtc aaccttaacg accgtcacaa
     1321 aacttccgag aggcaattcg acttctcgag caattatttc agcgactgtt gtcctgatca
     1381 actcgttgac ccgattgagg cgtaaaggca taaaagttac aaagtttttt tcactacttc
     1441 ctcatggaag caaagaagga tgtccccttc tttgattttt gtttccccgt caaaagtgat
     1501 tccgcattct tttccttggg tcacttcatc gacatcaacc ttgttttgct gaagattgat
     1561 gagtttcccg cgtcccaaaa caatatctgc gcgcgtcact tcaagaagct ccccgttttt
     1621 cattttcccg gagatgacct ctcccccgac aatcatatcc tttttcccag tcatgaagac
     1681 cgcaagaact ttgagctttc caagctccgt cctcacgata ttttcaccca acatcgcttc
     1741 aagccgcttt ttgatatcat caaccagctc atagataact ttatagcttt tgatttcaac
     1801 tttgttcatt tctgcgagcc ttttcgcgac cgttgtcggc tggatattga atccgaaaat
     1861 tacggcacct gaagtttgtg ccagcttcac gtcagattca gtgatatcac ccaccgcttg
     1921 gcgaatataa tttattccaa cttcatctga tttgatcgcc ccaagaatct gatcaacggc
     1981 ttcaagcgat ccttgcacgt ctgttttcaa gatgatattt atctttttga tattttggtc
     2041 ttcgatgctc ttgttcaaag tttgcgcgtc gaattttttc atcccgccaa agccgcccga
     2101 ttgcatttct ttctgcgaca tcttcgcgga tttcaaatca accgcaccct gcaaaacatc
     2161 gttcgtattc gggagttcgt ggaggccaat caccgtcact ggcgttgatg gtcccgcttc
     2221 tttgacttgc ttgcccttga agtcttcgag ccttcgcact cttccggcaa tggagcccgc
     2281 caaaatatcc tggccttcct tgagcgttcc ggtccgcacc agcaccgtcg acatatttcc
     2341 tttcttcgga tcaagatgcg attcaatgac cactccaagc ggcagccttt tgtagtccgc
     2401 cttgagattt tccacttccg ccaccagtaa tatcatatcc aaaagttcat cgatgccgat
     2461 attttgtttg gctgaaattt ccgccgccac aacatctccg ccccagtctt caatcacaat
     2521 cccgcgatca gccaattctt gtttcactct ctggacgttc gcgtttggct tgtcgacttt
     2581 gttgatcgcc accaccatcg gaatcttttt ttctttgaga tagttgatca cttcttctgt
     2641 ctgcggccgc acgccgtcat ccgccgccac aaccaggatg gcaatatcag cgagactcac
     2701 gccacgttcg cgcatcgctg aaaaagcctc atggcccgga gtatccacaa aagtgatagg
     2761 ttctcctttt tttttcactt gataggcact gatatgctgc gtgatcccgc ccgcctcttt
     2821 ttcaacaacg gacgttttcc ggatagtgtc cagaagagtc gtttttccat ggtcgacatg
     2881 gccaaggatc gtcacgaccg gcgcgcgatg tcgaaggccc acttccgtgg aactggcatc
     2941 cttctctttt ttcaaaattt cgtcgagttt ttccagtgtc atcgccgcct gcccgccggc
     3001 ttcaatatcc tcttccgcct cgaatcccaa gtcagaggca atgatcgccg ctgtttcaaa
     3061 atcgatctcg tcattgattg ttgccaggat cttgtttttc atcagctcct tgatgacatc
     3121 agaaattccc aaacccaaaa cttccgcgaa ttttttcacg gtcacccggg atggcatttt
     3181 cactttt
//
LOCUS       DATKGS010000051         4476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_130984, whole genome shotgun sequence.
ACCESSION   DATKGS010000051 DATKGS010000000
VERSION     DATKGS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130984"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VMQ48_01225"
     CDS             complement(<1..567)
                     /locus_tag="VMQ48_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUD08693.1"
                     /translation="MKKYFITTPVYYVNDKPHIGHAYTSVAADVLARFHKLQGDDVFF
                     LMGTDEHGLKIQKKAEEAGKEPQKFVDEISDGFKQLWDKLDIKYDAFIRTTDEKHKTA
                     VQKVLQTLFDKGAIYKGEYAGLYCVGCEQFKSESDLVNGRCPDHDTDCEIVKEESYLL
                     KMETLQEELIEKIENDEFKIRPEKYKKEI"
     gene            complement(572..1255)
                     /gene="rsmI"
                     /locus_tag="VMQ48_01230"
     CDS             complement(572..1255)
                     /gene="rsmI"
                     /locus_tag="VMQ48_01230"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HUD08694.1"
                     /translation="MTNFGKLYIVATPIGNLEDITLRALRVLKEADLILCEDTRVTKK
                     LLDRYEIQKPLLSYHHHSKLSRVEKIAEHLENGKNLALVSDAGTPGISDPGNELIAFL
                     RLNLKNAEIIPIPGTSAITTLASVAGINMSRFTFLGFPPHKKGRETFFREVAEMKYPI
                     VYFESPHRFLKNLELLEKMKPDAKLIVGRELTKMFEEILRGEIGEIRKNWEKKAEKEK
                     RGEFVIIAY"
     gene            1256..1726
                     /locus_tag="VMQ48_01235"
     CDS             1256..1726
                     /locus_tag="VMQ48_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08695.1"
                     /translation="MILTTHILAGALIGREINNPYAVAGLAVAMHFVLDLLPHGDYLD
                     KKSKLREFWKVAVDLTVGLGTVYAIFFIRDPISNNALYIKNIAIGIFFSLLPDGTTFM
                     YWKMGMKFLKPIYIFHQKLHRAPDFSPKRQFCLKNNLFDILISLIAIITLFSLR"
     gene            complement(1704..2741)
                     /locus_tag="VMQ48_01240"
     CDS             complement(1704..2741)
                     /locus_tag="VMQ48_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUD08696.1"
                     /translation="MNRTIEISSSTILRTIFILLLLWFLFLIWDIIILVFLALIIVSA
                     IDPIVDWCQKKKIPRGLAVLAIYILAIAAIGTAISFLVTPLISEIRGLGENFPNLVSK
                     YSGYFQGVQSYATSHNLQEQINNFSGSVTGRFSQIGTNVFSGTISFIGGVLSFLVVLS
                     VAFYMSVEEKGVKKFFASIAPDEHGEYVSGLVDRIQYKMGRWLLGQLALMVIIFAIDY
                     VGLLAIGAPYALVLAIMAGLLEIIPYVGPIVSAVVAASISFLHGPVTGLLVLALFAIA
                     QQLEGYVIAPLVMKKAVGLNPVVVILAILIGAKLGGVLGVIISVPIATVVSEVVKDLV
                     KKPEVSAERKE"
     gene            complement(2859..4142)
                     /locus_tag="VMQ48_01245"
     CDS             complement(2859..4142)
                     /locus_tag="VMQ48_01245"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUD08697.1"
                     /translation="MHFKKTTFKNGLRLITAPMKDTNTVTVVVAVAAGSKYESKKING
                     LSHFLEHMFFKGTKKRPKTKDIAESLDAVGGEFNAYTSTESTAYYAKVDKEHIGLAID
                     VVSDIFLNSKIDPKEIEKERGVILQEINMYEDNPMLSVQEEFENLLYGDQPAGWKVIG
                     TKENIARVSQKDFAGYMKNFYTAKNTVVSVVGNFGEMGESGVIKEIEKIFSKMRKGEV
                     NKKQKTTEKQARPELKIKHKKTDQAHLVLGFRGYNMFHKDRFALALLANILGGTMSSR
                     LFLSVRERLGLAYYVSAGDDESSDCGYFSVRAGVDTDKAKIEKTVKTIISEIKKMKAR
                     GVSEKELQKAKDNLRGKMALSLESSDEVAAFLAGQELGRREIKKPDEILRRIDKVKRD
                     DILRVARDIFRNENLNLAIIGPIEDKGFLERILKL"
     gene            complement(4182..>4476)
                     /locus_tag="VMQ48_01250"
     CDS             complement(4182..>4476)
                     /locus_tag="VMQ48_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08698.1"
                     /translation="RGALCEGRFKSIHIKNDSRLLYLASYVNCNSEVHGIFKSLKYKW
                     CSYLEYMGLCKDEICDKKIILEQFRGRKDYAEFAREAVWDAKQKKEDEKTFLE"
BASE COUNT         1133 a         1165 c          845 g         1333 t
ORIGIN      
        1 aatttctttt ttgtattttt ccggccggat cttgaactcg tcgttttcta tcttctcgat
       61 cagctcttcc tgcaaagttt ccattttcag aagatagctt tcttctttca ctatctcgca
      121 atccgtatca tggtccggac agcgtccatt gaccagatcg ctttcacttt tgaattgctc
      181 acacccgacg caatagaggc ctgcatattc gcctttatat atagcacctt tgtcaaaaag
      241 agtttgcaaa actttttgca cggcggtttt gtgcttctca tccgtcgtcc ggataaaagc
      301 gtcgtatttg atgtcgagct tgtcccaaag ctgcttgaaa ccatcagata tttcatccac
      361 aaatttttgc ggttcttttc cggcttcttc ggcttttttt tggatcttga gcccgtgctc
      421 gtccgttccc atcaaaaaaa agacatcgtc gccctgaagc ttgtgaaatc ttgccagcac
      481 atccgccgcc acgctcgtat acgcatgccc gatatgcggc ttgtcgttga catagtagac
      541 cggagtggtg atgaaatatt tcttcataaa atcaataagc gattattaca aactctcccc
      601 ttttctcttt ttctgccttc ttttcccaat tttttcggat ttcaccgatc tctcctctca
      661 aaatttcttc aaacatcttc gtcagttccc ggcctactat cagtttagca tcaggtttca
      721 ttttttccaa aagttcgagg ttcttcaaaa atcggtgcgg agattcgaaa tagacgatgg
      781 gatatttcat ttccgccact tctcggaaaa aagtttcccg gccttttttg tgcggcggga
      841 agccgaggaa agtaaatcgg ctcatgttta ttcccgcgac acttgcaagg gtcgtgatcg
      901 ccgatgttcc cggaatcgga atgatttcag catttttaag gttcagcctt aaaaatgcaa
      961 tcagctcatt accgggatct gaaatccccg gcgttcccgc gtcactgacc agtgccagat
     1021 tttttccgtt ctccagatgc tccgcgattt tttccaccct tgagagcttc gagtgatggt
     1081 ggtatgatag aagcggtttt tggatctcat atctgtccag cagcttcttc gtcacccgcg
     1141 tatcctcgca caagatcaaa tcggcttctt tgaggacgcg aagagcgcga agagtgatgt
     1201 cctcaagatt gccgataggt gtcgcaacaa tgtataattt tccaaaattt gtcatatgat
     1261 tctcactact cacatcctcg ccggtgctct catcggccga gaaataaaca atccatatgc
     1321 tgtcgcgggg ctggccgttg cgatgcactt tgtccttgat cttcttccgc acggtgacta
     1381 tctcgacaaa aaatcgaaat tacgcgaatt ctggaaggtg gctgtcgacc tcaccgtcgg
     1441 cttgggaacg gtttatgcga tcttttttat tcgagatcca atctcgaata atgccttata
     1501 cataaagaat atagccatcg ggatcttttt ctccctcctc ccagacggaa ccacttttat
     1561 gtattggaaa atgggaatga agtttctgaa accgatttat atcttccacc aaaaacttca
     1621 tcgcgctcct gatttttctc ccaagcgcca attttgcctc aaaaacaatc ttttcgatat
     1681 tttgatctcg cttattgcga tcatcactct tttctctctg cgctaacttc gggcttcttc
     1741 accaaatcct tcactacttc actcacaacc gttgcaatcg gaacggaaat aatcacgccg
     1801 agcactccgc ccagtttcgc gccaatgaga atcgccagga tcacaaccac cggattcaag
     1861 ccgacggctt ttttcatcac cagcggggca atcacatatc cttccaattg ctgcgcgatg
     1921 gcaaaaagag ccaaaaccaa gaggccggtg accggaccgt gcaaaaagct gattgaagct
     1981 gccacgactg ccgacactat cggcccgaca tagggaatga tttcgagaag gcccgccatg
     2041 atcgccagca ccaaagcata gggcgctccg atagccagca gcccaacata gtcgatcgca
     2101 aaaataatca ccatcagcgc cagctgcccc aaaagccagc gtcccatttt gtattgaatc
     2161 cggtcaacca atcccgaaac atattcccca tgctcatccg gcgcgatcga ggcaaaaaat
     2221 ttcttgacgc ctttttcttc gactgacata tagaaggcga cggacaaaac caccaagaaa
     2281 gaaagtaccc ctccgataaa agaaattgtt ccgctgaaaa cattcgttcc gatctgggaa
     2341 aacctgccag tcacgctgcc tgaaaaattg ttgatctgct cttgaagatt gtgggaagtt
     2401 gcgtagctct gcacgccctg gaaatatcct gaatatttgc tcaccagatt cggaaaattt
     2461 tctcccaatc cgcggatctc ggaaatgagc ggcgtaacca gaaaagaaat tgccgtccca
     2521 atggccgcga tagccaggat atatatcgcc agaaccgcca agccgcgagg aatctttttt
     2581 ttctggcacc aatcgacgat cggatcaatg gcactcacga tgatcaacgc caaaaaaacc
     2641 aggatgatga tatcccaaat gagaaaaaga aaccagagca aaagcaggat gaagatagtc
     2701 cgaaggattg tactggatga aatttcgata gtacggttca taataaattt ccaattatca
     2761 attttcaatt tacaatgaat tttcaatgac ccaatttaca atgtttgaaa attcagaaat
     2821 tgatcattga ttgaaaattg tgaattgaaa attgaaaatt ataacttcaa tatcctctct
     2881 aaaaatcctt tatcctcaat cggtccaata atagctaaat ttaaattctc gttccgaaat
     2941 atatccctcg ctactctcaa tatgtcatct ctcttcactt tgtcaatccg gcgcaaaatt
     3001 tcgtcgggtt tcttgatctc tcgcctcccg agttcttgcc cggccaaaaa tgcagctact
     3061 tcgtctgaag actcaagtga aagcgccatt tttccgcgca gattgtcttt ggctttctga
     3121 agttccttct cggaaacgcc tctcgctttc atttttttga tttctgaaat gatcgtcttt
     3181 actgtttttt caatctttgc tttgtccgta tccactcccg cccggacgga aaaatatccg
     3241 caatcggaag attcatcgtc gcccgcactg acataatacg ccaatcccaa tctctcgcgc
     3301 accgaaagaa acaaccggct actcatcgtc ccgccgagaa tattcgcaag aagcgctaaa
     3361 gcaaatctgt ctttgtgaaa catattgtat ccgcgaaatc ccaaaaccag atgcgcctgg
     3421 tcggtttttt tgtgtttgat tttgagctcc ggcctggcct gcttctcggt cgttttttgt
     3481 ttcttgttca cctcaccctt tcgcatcttc gaaaatattt tttcgatttc cttgatcact
     3541 ccgctctctc ccatttcccc gaaatttcca accactgata ccaccgtatt cttggcagta
     3601 tagaaatttt tcatatagcc cgcaaaatct ttttgggaaa ctcgtgcgat attttctttc
     3661 gtcccgatca ctttccaccc cgccggctga tcgccataga gaagattttc aaattcttcc
     3721 tggacggaaa gcatcggatt gtcttcatac atattaattt cctgaaggat cactccccgc
     3781 tctttttcga tttctttcgg gtcgatcttt gaattcaaaa aaatatccga gacgacatca
     3841 atcgcgaggc cgatgtgctc tttgtcgacc ttggcataat aggccgtgct ttctgtggag
     3901 gtataggcgt tgaattcccc gccaaccgca tcgagcgact cggcgatgtc ttttgtcttc
     3961 ggccgctttt ttgtcccctt gaaaaacata tgctcaagaa aatgcgacag gccgtttatt
     4021 tttttcgatt catacttcga tccggccgcc accgccacaa ccaccgtcac cgtgttcgtg
     4081 tctttcatcg gcgcggtgat caatcgcaat ccgtttttga atgtcgtttt tttgaaatgc
     4141 attagcgtca cgacaagagc tcaactcttg tcagtaaaaa tttattcgag aaaagttttt
     4201 tcatcctcct ttttctgttt cgcatcccaa acagcttctc ttgcaaattc cgcataatcc
     4261 tttctgccgc gaaactgttc caatattatt ttcttgtcgc atatctcatc cttgcaaagt
     4321 cccatgtatt ccagataact acaccattta tatttcaagg atttaaaaat tccatgcact
     4381 tcactgttgc aatttacata cgaagccaga tacaacagcc tgctgtcgtt tttgatgtgg
     4441 atggatttga agcggccttc acataaggct cccctc
//
LOCUS       DATKGS010000052         3093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_75716, whole genome shotgun sequence.
ACCESSION   DATKGS010000052 DATKGS010000000
VERSION     DATKGS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75716"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VMQ48_01255"
     CDS             <1..332
                     /locus_tag="VMQ48_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HUD08699.1"
                     /translation="FISSANSGRVLENAVFLELKRRGRDVYYFKEKNECDFIIREKNR
                     PDMAIQVTKRLDAQNDNREINGILEAMEKLKLKKGIIITEDQEEERIIKGKKIKIVPV
                     YKWLLED"
     gene            349..498
                     /locus_tag="VMQ48_01260"
     CDS             349..498
                     /locus_tag="VMQ48_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08700.1"
                     /translation="MEKDYAIKVNGVSKTFRIPHEKITTIRGAFVSALKSHGYEEFKA
                     LVDAC"
     gene            554..1807
                     /locus_tag="VMQ48_01265"
     CDS             554..1807
                     /locus_tag="VMQ48_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD08701.1"
                     /translation="MKKPYIKRKLEDTISKYLEKREILAIVGPRQAGKTTLLKKVQSE
                     LKNSVFLSFEDREDLELFERDVKSFAKKYFDRQYVFIDEFQYSKHGGKRLKYLYDTYP
                     DVKIVISGSSAIDLTVQAIKFLVGRVFVFNLYQLDFEEYLLFKSKEGLASYGKYKKSF
                     DIQKDRLDFPQIKDSESKELKKILDDFILWGGYPRVALAQSDDERKTVLKNIYNTYFL
                     RDIRDVLGLTDDFRLSKLVKLLAVQIGQLVSYNELGSSAGYDYLTLKKYLNVLEKTFI
                     CQPIRPYFVNKRKEITKNPKIYFFDTGLRNHIVGNFSSLNERDDKGFLLENYIFNQLV
                     KKDMTVNFWRTKQKDEVDFVVETGGRKIPLESKSVFQKAQMPKGIGMFVSENNSELAV
                     ILNETSSGRMMENKTKVYILPHWII"
     gene            1977..>3093
                     /locus_tag="VMQ48_01270"
     CDS             1977..>3093
                     /locus_tag="VMQ48_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD08702.1"
                     /translation="MINPEIIKELILRQRKIFLDTSDAISREIIVEKKFQKSVSLREA
                     VIITGVRRSGKSYLMRLIWKKIVAERKMPEKNFLYFNFEDEKLLKFSASDFETLLESY
                     FEVIEPEKSKKIYLFFDEIQNIPGWEKFINRLREDRGYKIFITGSNAALLSKEIGTAL
                     TGRNFPVSLFPLSFREFCGFKLKREFMKEDFYGAEEKAKLKKILKSYLKNGGFPEVVI
                     QNFRPLLQEYLKNIIYRDIILRHKIKNEASLREISAFVISNVGVNLSLEKISRMTKVK
                     NLMTVKNYLSHLEDTFLFYAVPKFSYSVKDQIYNPDKIFVVDTGMYNEVAFISSANSG
                     RVLENAVFLELKRRGRDVYYFKEKNECDFIIREKNRPD"
BASE COUNT         1048 a          524 c          672 g          849 t
ORIGIN      
        1 ctttcatttc gagcgccaat tcaggaaggg ttctggaaaa cgctgttttt ttggagctca
       61 agaggagagg tcgtgatgtc tattatttca aggaaaaaaa cgaatgcgac ttcatcattc
      121 gggaaaagaa tcgtccggac atggccatcc aagtgacgaa aagattggac gcgcaaaatg
      181 ataacaggga aatcaatgga attctcgagg ctatggaaaa acttaaattg aaaaagggga
      241 ttataataac ggaagatcaa gaagaggaaa gaataatcaa aggaaaaaag ataaagatcg
      301 tacctgttta caagtggctt ttggaagact agaataaaca aagttattat ggaaaaagac
      361 tacgcaatca aagtcaatgg ggtttccaaa actttccgca tcccgcatga aaagatcacg
      421 acgatccgcg gggcttttgt gagcgcgctc aaatcccacg gctatgaaga attcaaagcg
      481 cttgtcgatg cttgttgaca gattataaaa tataaactaa aattatatta ttatatagtt
      541 ttagtaataa cgcatgaaaa agccttatat caagcgaaaa ttggaagata ccatttctaa
      601 gtatttggaa aaaagggaaa ttttggctat tgtcggtccc aggcaagcgg ggaaaacaac
      661 attgctgaaa aaagtgcaat ccgaactcaa aaactcggtt tttctcagct ttgaggatcg
      721 agaggatttg gaactttttg aacgcgatgt gaagagtttt gcgaaaaaat attttgaccg
      781 tcaatatgtc tttatagatg aatttcaata ttccaagcat ggagggaaaa gactgaaata
      841 tctttatgac acatatccgg acgtcaaaat tgttatttcc ggatcatccg cgattgatct
      901 cactgtgcag gcgataaaat ttttggttgg tcgggttttt gtcttcaacc tgtatcagct
      961 tgattttgaa gaatacctgc tgttcaagag caaagaaggg ttggcgtcct atggaaaata
     1021 caaaaaatct ttcgacattc agaaagacag gcttgatttt ccgcagataa aagattcgga
     1081 aagcaaggaa ttgaaaaaaa ttctggatga ttttatactt tggggaggat atccccgagt
     1141 agccctcgcc caaagtgacg atgaaagaaa aacggttctg aaaaatatct ataacaccta
     1201 ttttcttcgg gatatcaggg atgttctcgg attgacggat gattttcggc tgtcaaaact
     1261 tgtcaaactg ctggccgtcc aaatagggca gcttgtttcg tacaatgaac ttggaagttc
     1321 agccggatat gattatctca cgctcaaaaa atatctgaat gttttggaaa aaacatttat
     1381 ttgtcagcca atccggccgt atttcgtaaa caagagaaaa gaaatcacga aaaatcccaa
     1441 gatctatttt tttgataccg ggcttcgcaa ccatatagtg ggcaattttt ccagtctgaa
     1501 cgaaagggat gacaaaggtt tcttgctcga aaactatatt ttcaaccagt tggtaaaaaa
     1561 agatatgaca gtgaatttct ggaggacaaa acaaaaagat gaagtcgatt ttgtcgtgga
     1621 aacgggtgga agaaaaattc cacttgaaag caaatccgtt tttcaaaaag cgcaaatgcc
     1681 gaaaggaatt gggatgtttg tttcggaaaa caattcggaa ctggccgtga tattgaacga
     1741 aacttcaagc ggcaggatga tggaaaacaa aacgaaggta tatattttgc ctcattggat
     1801 aatttgatta tttattatgc tggaaaagga cctcgccatc aaagttgaaa acgtcagttt
     1861 tgaggtgaaa aaaaggggaa tttttcggtc gggatataga gatgaattta tattgccatt
     1921 gtaatgtata gtacgatata ttataataga aatataatat attctgaagc aaaagaatga
     1981 tcaatccaga aataatcaaa gaattgattt tgcgccagag gaagattttt ctggatacca
     2041 gcgatgcgat ttcgcgcgaa attattgttg agaaaaaatt tcaaaaatcc gtttctctcc
     2101 gggaggccgt cataatcacc ggagttagaa ggtcgggaaa atcatacttg atgcgcctga
     2161 tctggaaaaa gatagtcgcg gagcgcaaaa tgccggaaaa aaatttcctg tatttcaatt
     2221 tcgaagacga gaaacttttg aaattcagcg cgtctgattt tgaaacgctg cttgaaagct
     2281 attttgaggt cattgagccg gaaaaaagca aaaagatata ccttttcttc gatgagatcc
     2341 aaaacattcc cggttgggaa aaattcatca atcgcctcag agaagacaga ggatacaaga
     2401 tattcattac cggttccaac gcagcccttc tttccaaaga gattggaacg gctctcactg
     2461 gcagaaattt tccggtgagc cttttcccct tgtcttttcg tgaattttgc ggtttcaagc
     2521 tcaaaagaga attcatgaaa gaagattttt acggcgctga agaaaaagca aaattgaaaa
     2581 aaatattaaa aagttatctg aaaaatggag gtttcccgga agtggttatt caaaattttc
     2641 gtccgcttct tcaggaatat ctcaaaaaca tcatttatcg ggacatcatt cttcgccaca
     2701 aaatcaaaaa cgaggccagc ctccgagaaa tttccgcttt cgtcatatcc aatgttgggg
     2761 tgaatttgag tctggaaaaa ataagccgga tgaccaaggt gaaaaatttg atgactgtca
     2821 agaattattt gtcccatctc gaagacactt ttctttttta cgcggttccg aaattcagct
     2881 attccgtgaa agatcaaatc tacaatccgg ataaaatttt tgtcgtggat accggcatgt
     2941 acaacgaagt tgctttcatt tcgagcgcca attcaggaag ggttctggaa aacgctgttt
     3001 ttttggagct caagaggaga ggtcgtgatg tctattattt caaggaaaaa aacgaatgcg
     3061 acttcatcat tcgggaaaag aatcgtccgg aca
//
LOCUS       DATKGS010000053         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_31483, whole genome shotgun sequence.
ACCESSION   DATKGS010000053 DATKGS010000000
VERSION     DATKGS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31483"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..460
                     /locus_tag="VMQ48_01275"
     CDS             260..460
                     /locus_tag="VMQ48_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08703.1"
                     /translation="MTDKLNNQQNADKAKPRKDNIHCGDPPSNGPMKTEAKNTMPILD
                     NVSARSLSCVSESRINFDFILL"
     gene            490..1764
                     /locus_tag="VMQ48_01280"
     CDS             490..1764
                     /locus_tag="VMQ48_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HUD08704.1"
                     /translation="MKKETKKEKLHPVRSNPAKRDAESSIRISNGVKIKKPEEIPAME
                     NSTQPPASDDSENIDPAKISEIMSMNQCVYCTSRNIVKRGKRKNKLEEVQLYICQDCG
                     KTFTGKKIKGKKYPTRMILDGVSYYNVGFSLEESAKFLERSYGVKVDASTISHWVKEL
                     ADLCSYGRLREFGLKMYSPAQVVQSFQMYHRQIYRFRYHRAKIALALQEFGHYRFAPL
                     REYLDSIATDCPHYLFKESDRSSEIKAEMDLHEVRITEKQNRATQLAQLVLQAVADNK
                     WRHDALQKFMLANDSVTVATEVPVFFEREDYDWMKDNLKFEIPFEMEKTITGHIDFIQ
                     IRNKSIHILDYKPNADKARPVAQLTFYALALSRLTGLRLFDFKCAWFDEKNYYEFFPL
                     HVVYKKKKRGRGRGKEEEPKTQITHVENITQT"
     gene            1850..>2266
                     /locus_tag="VMQ48_01285"
     CDS             1850..>2266
                     /locus_tag="VMQ48_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUD08705.1"
                     /translation="MFRKSRFIKKKPVRGFRDLEIYQKTQEMAAQVVRLVPEILKDKN
                     YPTEKKVIEMALDIPSQIARSHSQRYEDRPGALQRMEEVLQRCDWMIVHLEQIREIYV
                     ADEGEKAALTEIIRRYGTVRVKIRNLHKAWKRWGEEN"
BASE COUNT          716 a          560 c          520 g          470 t
ORIGIN      
        1 aggtgctagg ggacatattc acgcgaaatt aggcaacttt tcgtgagatg ggcgaaaaga
       61 cgagccaatt cgtgagatga aagataaaca aaaagcggct gacaaaaaaa ccaaccgcaa
      121 aaagtattcc gggatgaacc aatttatttt ttcttaaggg ctatgacaac cagagcgaaa
      181 gcaatcacaa caagagcgcc gagaatatag agattggtca taatttgatt aatattgtta
      241 ttcataaaat ttttccttaa tgaccgacaa gctcaacaac caacaaaatg cggataaagc
      301 caagcccaga aaagacaata tccactgcgg cgatccgccg agtaatggtc cgatgaaaac
      361 agaagcaaaa aatactatgc cgatattgga taacgtctcg gcgagaagtt tgagttgtgt
      421 ttctgaaagc cgcataaact ttgattttat tttattatag caaaccaaaa aacagtgtca
      481 accgccaaaa tgaaaaagga aacaaaaaaa gaaaaacttc accccgttag aagtaatcct
      541 gccaagcgag atgccgaatc ttcaattcgg atttctaacg gggtaaaaat caaaaaaccg
      601 gaagaaatcc cggccatgga aaacagcacc cagccgcccg cttccgacga ttcggaaaat
      661 attgatccgg cgaagatttc cgaaatcatg tcgatgaacc agtgcgtcta ttgcacgagc
      721 cggaatattg tgaagcgcgg caaaagaaag aacaaactcg aagaagtgca attgtatatc
      781 tgccaggact gcggaaaaac tttcaccggg aaaaagatca aaggaaaaaa atatccgacc
      841 cggatgatcc tcgacggcgt gagctactac aacgtcggat tttcgctcga agagtccgcc
      901 aaatttttgg aacggagcta tggcgtgaaa gtggacgctt ccaccatctc gcactgggtg
      961 aaagaactcg ccgacctttg ctcctacggc cgcctgcgcg aattcgggct caaaatgtat
     1021 tctcccgccc aagtcgtgca gagtttccag atgtatcacc gccagatcta ccgcttccgc
     1081 taccaccgcg ccaagatcgc ccttgccctt caggaattcg ggcactaccg gtttgctccg
     1141 cttcgcgaat atcttgactc catcgccacc gactgcccac actatctttt caaagagtcc
     1201 gaccgctcct ccgagatcaa agccgagatg gatcttcacg aagtccggat cacggaaaaa
     1261 caaaatcgcg cgacgcaact cgcccaactc gttttgcaag ccgtcgctga caacaaatgg
     1321 cgccacgacg ctttgcagaa atttatgctt gcgaacgact ccgtgacggt ggcgacggaa
     1381 gtgccggttt ttttcgagag ggaagactac gactggatga aagacaattt gaaattcgaa
     1441 attccgttcg agatggagaa aacgatcacg ggtcatattg atttcataca gatccgcaac
     1501 aagtccatcc acattctcga ttacaaaccg aatgccgaca aagcccggcc cgtcgcccag
     1561 ctgacattct acgccctcgc gctttcccgc ctcacggggc tgcgcctctt cgatttcaaa
     1621 tgcgcctggt tcgacgaaaa aaattattat gaatttttcc cgctccacgt cgtctacaaa
     1681 aagaaaaaaa gaggccgggg acgggggaag gaagaagaac caaaaactca aattacccat
     1741 gttgaaaata taacgcagac atgataatct aaaatccgat atccgatatt tagaagccaa
     1801 cattcaatgt tagggcggct ccaacattga atgttaggca acaattatta tgtttcgcaa
     1861 atcaaggttc atcaaaaaga aacccgtgcg ggggttccgc gatctggaga tataccagaa
     1921 gacgcaggaa atggcggcgc aggtcgtgcg gcttgttccc gaaattttga aagacaaaaa
     1981 ttatccgact gaaaagaaag tcatcgaaat ggcgcttgat attccgtccc agattgcccg
     2041 ttcccattcg caaagatacg aagaccgtcc gggcgccctc caacggatgg aagaagtcct
     2101 tcagcgctgc gattggatga tcgtccatct ggagcagatc cgcgaaatct acgtcgcaga
     2161 cgaaggcgag aaagcggcgc tcacggaaat catccgccgc tacggaaccg tccgcgtgaa
     2221 gatccgcaat ctccacaaag cctggaaaag atggggggag gaaaac
//
LOCUS       DATKGS010000054         2706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_60230, whole genome shotgun sequence.
ACCESSION   DATKGS010000054 DATKGS010000000
VERSION     DATKGS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60230"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..905)
                     /locus_tag="VMQ48_01290"
     CDS             complement(366..905)
                     /locus_tag="VMQ48_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08706.1"
                     /translation="MILGIKELHRLVDEQKLVENLCEREMKNPEGAGFDLQMGEIFEL
                     EGDGYLGIEERNTPETKSVAKFEKGKGNSFVFEPGKYYLIKTMEKVNLPIDLMAVIFP
                     RTTLFRSGLALFNGQVAPGYCGELTFGLCNLGKSKIKIDLGARVVHITFHQVLGEGNQ
                     YRGQWQGGRVSTDGKEVQV"
     gene            complement(930..1931)
                     /gene="thyA"
                     /locus_tag="VMQ48_01295"
     CDS             complement(930..1931)
                     /gene="thyA"
                     /locus_tag="VMQ48_01295"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF002496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HUD08707.1"
                     /translation="MSEKKHPEYQYLDLMKDILENGSDKKLFLTPEVQEQYKKQGEKL
                     PFIRSVFGRVIRFDLNEGFPLLTTKKVFYRGIFEELLWFLSGDSNIKYLVDRDVHIWD
                     EWAWKKYHKYCLENDPKKDMDQEAFIAKLKTEPTDSEFVKKWGDLVTIYGRMWRKWPA
                     SDGHLIDQLGYCIKGLKDKPYRKSYVVSAWNPDFIYAMASPENKNEVPPFCHTTFQFS
                     VVNDKLNLGMYQRSADLFLGVPFNIASYALLLQMVSQVVGIPMGEFVHFFGDVHIYSN
                     HFEQVKEQLSREPKPFPKMIINPEVKNINDFKYEDFTLEGYDPHPTIKAEIVNVGGS"
     gene            complement(1938..2501)
                     /locus_tag="VMQ48_01300"
     CDS             complement(1938..2501)
                     /locus_tag="VMQ48_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HUD08708.1"
                     /translation="MSETKKPAFKRPSWDAYFMALAKLAASRSTCLSRPTGCVIVRDK
                     QILSTGYNGSMPGVAHCSDEGVCYRRSIGASDDGKYDYCRSIHAEANAIALAARQGVS
                     VKGATVYSTLFPCYVCTKLMASAGISEIVFELSYESWNKERDKHWKEATGQAKLKLRE
                     YKIDEKDKIFFEYFIQNETSRRRLKSE"
BASE COUNT          740 a          652 c          509 g          805 t
ORIGIN      
        1 gggggggcac tcacaaaaca tcttcgagtc tgttgcgagc tcgacatgaa cctccatccc
       61 gattgttggc ttgtatttca tataattatt ctagcaaaaa tgactgaaaa aataaaggcc
      121 ccgagaggcc tttggtaaaa agtataaaga aattagttaa gtaccggctc aacactgaaa
      181 acgccgagtt tagcgtgttc accgattata tttctaaagg aacacttttg ggaaaattca
      241 tgtccgcact cgccacaagt ccacccggag caatgccaca tcagaatttc tatgacaggc
      301 ggataaatat tcacgggata ttctttgcta tgattttcca ctttttttcc ctccttgttt
      361 agcatctaaa cctgaacttc tttcccatcc gttgagacac gaccgccctg ccattggccg
      421 cgatactgat tgccttcacc aagaacttga tggaaagtga tatgcaccac tctcgctcca
      481 agatcaatct tgattttgct ttttccaaga ttgcaaagcc caaaggtcaa ctcaccgcaa
      541 tatcccggcg caacctgacc gttgaaaagc gcgagtcccg atcgaaaaag agttgttcgc
      601 ggaaaaatga ctgccatcag atcaatcggc aaattcactt tctccatcgt cttgatgaga
      661 taatactttc ccggttcaaa aacaaacgaa ttgcccttcc ctttttcgaa tttcgccacc
      721 gatttcgttt caggtgtgtt tctttcctca atgccaagat agccatcgcc ctcgagttcg
      781 aaaatttctc ccatctggag atcaaaaccg gcgccttcgg gatttttcat ctcccgctcg
      841 cacaaatttt caaccagttt ttgctcatcg accaaccgat gaagctcttt gatgccaaga
      901 atcatggtat atacgcatta ataattattt taagatccgc cgacattcac aatttccgct
      961 ttgattgtcg gatggggatc atatccctcc agggtgaaat cttcatattt gaaatcatta
     1021 atatttttca cttccgggtt gatgatcatt ttgggaaaag gttttggttc gcgcgaaagt
     1081 tgttccttga cttgctcgaa atgattgctg tatatatgga cgtccccgaa aaaatggaca
     1141 aattctccca ttggaatccc taccacctgc gaaaccatct ggagaagcag cgcatagctc
     1201 gcaatattga acggcacacc cagaaaaaga tcggcgctcc tttgatacat tcccaaattg
     1261 agcttgtcgt tcacgaccga gaactgaaaa gtggtatggc aaaaaggagg cacttcattt
     1321 ttgttctccg gcgaagccat tgcgtatatg aaatccgggt tccaggccga aacgacatag
     1381 gatttccgat agggcttgtc tttgaggcct ttgatgcaat aaccaagttg gtcgatcaga
     1441 tgtccgtcgc tcgccggcca tttgcgccac atccggccat agattgtcac caaatctccc
     1501 cactttttca caaattcgct gtcagtcggt tctgttttca atttagcgat gaacgcttct
     1561 tgatccatat cttttttggg atcattttcc aggcaatatt tgtgatattt tttccaagcc
     1621 cactcgtccc agatatgaac atcccggtca accagatatt tgatattcga atccccgctc
     1681 aaaaaccaaa gtagttcttc aaatattcct cgatagaaaa ctttcttggt ggtgaggagc
     1741 ggaaaaccct cattcaaatc aaaacggatc acccttccga aaacagaacg gatgaacggc
     1801 aacttttctc cctgcttttt gtactgttcc tgcacttccg gagtcaaaaa caactttttg
     1861 tccgaaccgt tttccaaaat atccttcatg agatccaggt attgatattc gggatgtttt
     1921 ttttcgctca tattttttta ttcgcttttt agccttcttc ttgatgtttc gttctgtata
     1981 aaatactcaa aaaatatttt atctttttca tcaatcttat attccctcaa cttcaattta
     2041 gcttgccctg tcgcttcttt ccaatgctta tctctttcct tattccaaga ttcataggac
     2101 aattcgaaga caatttccga aatacccgca ctggccatta gtttggtgca tacatagcat
     2161 ggaaaaagag tggaataaac cgttgcacct ttcacactta cgccctgccg agcggccagc
     2221 gcaatcgcgt tggcttcagc atgaatactg cgacaataat catattttcc atcatctgat
     2281 gcaccaatac tgcgacgata gcagactcct tcatcactac aatgtgcaac tccaggcatc
     2341 gacccattgt atccggtgga caaaatctgt ttatcgcgga caataacaca gcctgttggt
     2401 cgcgacaaac aggtcgaacg cgaagcagcc agtttagcga gagccatgaa gtacgcatcc
     2461 cacgatggac gtttgaaagc aggttttttt gtttcggaca ttttatttgt tagttaaaaa
     2521 aggcaaaaat ttaggcagaa ttaatcattt tcttcagctt ctgtggtgaa accgtgatga
     2581 cgtacaaatc ggcaaaaata ttggatgaat ttagaatttt atcttttcga ggaattattt
     2641 gccaatgaag atgtgcaatg gaggctccag ctttttcccc taaattaaga ccgatattga
     2701 accccc
//
LOCUS       DATKGS010000055         5681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_31600, whole genome shotgun sequence.
ACCESSION   DATKGS010000055 DATKGS010000000
VERSION     DATKGS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31600"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VMQ48_01305"
     CDS             complement(<1..415)
                     /locus_tag="VMQ48_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08709.1"
                     /translation="MKKLKISISYPPLNSAKGVPLLTQNRQFQWFSDPTYIYPVIPAS
                     AATLLKANSYEVFWDDAIAENLTYEEWKARLISEKPDVVVIETKTPVIKRHWQIIDDL
                     KKESLEISAKGGPASGWGNPKIVLMGDHVTALPEES"
     gene            complement(440..1417)
                     /locus_tag="VMQ48_01310"
     CDS             complement(440..1417)
                     /locus_tag="VMQ48_01310"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD08710.1"
                     /translation="MTRYSFIIPVKEINEYVRESIPAILSLGRNDFEILIYPDEIASD
                     IWPKTRQIASGSVGPAQKRNLAIRDAKGEILVFIDDDAYPKNNFLEILGKDFASDDIV
                     AVGGPAMTPKESKFWQKVSGATFLSSLSGGFPERYRPVGKKKFVADWPSVNLSIRKKI
                     FDELGGFGGDYWPGEDTKLGFDLLMKKNISILYDPELVVYHHRREGLGKHVKQISAYG
                     LHRGFFAKRYSKTSFNWRYFMPSLFVLFIIFGGTLAYSSKLFLNLYILGWIIYFVALL
                     NAFYDIWKHEKNILVTLAASYYIFLTHIFYGVRFIQGFLFTRELKSKLR"
     gene            complement(1436..2713)
                     /locus_tag="VMQ48_01315"
     CDS             complement(1436..2713)
                     /locus_tag="VMQ48_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HUD08711.1"
                     /translation="MFKSKFLKNNAIFMAGTLVGGLLGYAFHFVVSRQISVAQYGELQ
                     SLLSTMLIFGVFNSALSYFTIKHTSVFAAHQDFEANREFTSYLASRVFKLTLAILLAL
                     LIASPLLANFLHFSSTIGFIVVSLATFFSTMTIIYFEILRGWQKFFLLSLAGIATAFV
                     KFASGAILASIAHNTAAVSFSLLTAAFAGWYLAKYWSRKQITGGNIPDAGTGWKHKYF
                     SGTNIRRSAMNIFFFSLALILVSNLDVILVKYFSSAETAGYYGAFALLGKIVLWLNLS
                     VAGVLLPDACADGHTGKRPDKKTLLNSYTLMALIALGLITIYYFIPNLVVNLFFGKKY
                     ILDTQILWLFGFMSFLLSILTFEANLSFAKHDFRVVYFLATTVILMVVSLARYHTNLQ
                     QIVLAFSASFFVGYLLVLAANLTHEKRRINLPS"
     gene            2853..5000
                     /gene="recQ"
                     /locus_tag="VMQ48_01320"
     CDS             2853..5000
                     /gene="recQ"
                     /locus_tag="VMQ48_01320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HUD08712.1"
                     /translation="MINLLKNNFGFDRFRPLQEEIIEQALLGKDAFVLMPTGGGKSLC
                     YQLPALKMPGLTLVVSPLIALMKDQVDDLQANGIAAAFVNSTLSPAEIDRTQADARTG
                     KLKILYAAPERLANPGFQNFLAGLKISLIAIDEAHCISEWGHDFRPDYRNLKMLRNIF
                     PNVPVMALTATATEKVREDIIRQLSLEKAKIFISSFNRPNLSYAVLPKKDSYDQLIEI
                     LQGHKNESVIVYCFSRKDTENLATDLRKEGFKALAYHAGLESGKRRKNQEKFIRDEAH
                     IIVATIAFGMGIDKPDVRLVVHYSLPKSIEGYYQETGRAGRDGLPSRCILFYSYGDTV
                     KQQFFIRQIEDESERENAYKKLNQMVEYCELASCRRSHLLSYFGEKYEAEKCDSCDVC
                     LSPKTEFDATVISQKILSAMVRTGQRFGASYIVDVLTGAKNKKLLDRGHQKLSVYGIA
                     GDFSKEELRNIIGQLLGKKLIVKTGEEYPILALGERGKAFLKNRENILLQKSETGRET
                     IRKSAAFEADYDQKLFDKLRLLRKNIADEKGVPPFVVFGDLALTQMATYFPQSEDSFA
                     KISGVGEEKLKKYGKIFTEVIRAHAKKNHLAEKSIPVGRSPQVRRAIRTGSTYQETKK
                     LALQKISIEKMAKTRGMTVGTITSHLEKLTAAEEEIDIEYLRPPANKMEKIKSAFRKS
                     GGTALSPVREILGEEFSYDELKVARMFLDRIVI"
     gene            5177..5449
                     /locus_tag="VMQ48_01325"
     CDS             5177..5449
                     /locus_tag="VMQ48_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08713.1"
                     /translation="MKIILKINEEKNIKLSLFEGKREVDGLEWKDENSLSRVLLSNLD
                     KLLQKNGVGLDKIASYNIISKVPQKWTTYRIADIALKTLKIGTSAR"
BASE COUNT         1700 a         1168 c         1399 g         1414 t
ORIGIN      
        1 gggattcttc ggggagcgcg gtgacgtgat cgcccatcag gacaattttc ggatttcccc
       61 agccagaggc tggtccgcct ttggcggaaa tttccaaact ttcttttttc aaatcgtcga
      121 ttatttgcca gtgtcgtttg atgacagggg ttttggtttc aataacaacg acatccggtt
      181 tttcgctgat tagccttgct ttccattctt cataagttaa attttcagcg atggcgtcgt
      241 cccaaaaaac ttcatagctg ttcgccttga gcaatgtcgc ggcgctagcc gggatcacgg
      301 gataaatata cgtcggatcg gaaaaccact ggaactggcg gttctgcgtg agtaagggta
      361 cacccttggc ggaatttaat ggtggatagg atattgatat ttttagtttt ttcataattt
      421 tttccaaagc tcggcttttc tatcgtaatt tgctttttaa ttctcttgtg aataagaatc
      481 cttggatgaa tctgactcca tagaagatat gggtgaggaa aatatagtag gacgcggcga
      541 gagtgacgag gatgtttttt tcgtgcttcc agatgtcata gaaagcgttg aggagggcga
      601 cgaaatagat gatccagccg aggatataaa gattcaaaaa taattttgat gaataagcga
      661 gcgtgcctcc aaaaatgata aacaaaacaa acagactcgg catgaaatat ctccaattga
      721 aagaagtttt ggagtatctt ttggcgaaaa atccgcggtg aagtccatag gcgctgattt
      781 gcttcacgtg ctttccgagt ccctcgcgcc gatgatgata gacgacaagc tccgggtcat
      841 aaagaatact gatgtttttt ttcatcaaaa ggtcgaatcc cagttttgtg tcttcgcccg
      901 gccaatagtc tccgccaaag ccgccgagtt catcaaaaat ttttttccgg atggaaaggt
      961 tcacgcttgg ccaatcggcc acgaattttt tctttccaac tggccggtat ctttccggaa
     1021 agccgccgga aagggagctc aagaatgttg ctccggaaac tttttgccaa aatttgcttt
     1081 ctttcggagt catggctggt ccgccgacgg caacaatatc atcgctcgcg aaatctttgc
     1141 cgagaatttc caaaaaatta tttttgggat aagcgtcgtc gtcaatgaaa accaatattt
     1201 cacctttggc gtcgcgaatg gccaaatttc ttttttgcgc cggtccgacc gagccacttg
     1261 cgatttggcg ggttttcggc cagatgtcac ttgcgatttc atccggataa atcagaattt
     1321 caaaatcatt tcggccaagc gaaagaatcg cgggtatcga ttcccggaca tactcgttga
     1381 tttctttgac tggaataatg aagctgtacc ttgtcattga aaggggctgt gaaaattagg
     1441 aaggcaaatt gatccggcgt ttttcatggg taagattcgc agcaagcacg agcagatagc
     1501 cgacgaaaaa tgacgcggaa aaggcgagga cgatctgctg gaggttggta tgatatctag
     1561 cgaggctcac gaccatcaga atcactgttg ttgccaggaa atataccact cgaaagtcat
     1621 gcttggcaaa ggacaggttg gcttcaaaag ttaatatgga aagcaagaag gacataaagc
     1681 cgaatagcca aaggatctgg gtgtcgagaa tatatttttt tccgaaaaaa agattgacga
     1741 ctaaattggg gataaaatag taaatggtga ttaaacccag tgcgataagc gccatcagag
     1801 tatatgaatt caatagagtt tttttgtccg gtcttttccc cgtgtggccg tcggcacaag
     1861 cgtccggcag cagaacgccg gcgacagaca gattgagcca gaggacgatt ttcccgagca
     1921 gggcgaatgc gccataatat ccggcagtct cggctgaaga aaaatatttg accaaaatga
     1981 catcaagatt cgagacaagg atgagagcga gcgagaaaaa gaaaatattc atggctgatc
     2041 ttcggatatt tgttcccgaa aagtatttgt gcttccatcc ggtaccggca tccggaatat
     2101 ttccaccagt gatttgtttt cggctccagt attttgccag ataccagccc gcaaaagcgg
     2161 cagtgaggag tgagaaagaa actgcggccg tgttatgcgc tattgatgcc aaaattgcgc
     2221 ccgaggcgaa tttcacgaag gcggtggcga ttccggcaag actaagcagg aagaattttt
     2281 gccatccgcg caaaatctca aagtatatga tcgtcattgt tgagaaaaaa gtggcgaggc
     2341 tcacgacgat gaagccaatc gtgcttgaaa aatggagaaa atttgccaaa aggggactcg
     2401 cgatgagtaa ggcaagcaat atcgccaagg tcaatttgaa aacacgagat gccaaatagc
     2461 tggtgaattc tcgattggcc tcgaaatctt gatgcgcggc aaaaaccgaa gtatgcttga
     2521 ttgtgaaata ggaaagcgct gaattgaaaa ccccgaaaat gagcatcgtc gaaagaaggc
     2581 tttgcagctc cccgtattgg gcgactgaaa tctgccttga tacgacaaaa tggaacgcat
     2641 agcccaaaag gccgccaacg agcgtcccgg ccatgaaaat agcgttgttt ttgagaaatt
     2701 tgcttttgaa catgggggtt tctattgatt tgatattagc atatcgttag gaaaatgcca
     2761 taaaataagc gcaaaagaaa aagtccgaaa ctttatccac agtagacaga atggtgatat
     2821 agtgataata tttgcataga acaagttttt ttatgataaa tcttctcaaa aataatttcg
     2881 gttttgatcg gtttcgtccg ctgcaggagg aaatcatcga gcaagcgctt cttggaaaag
     2941 acgcgtttgt tttgatgccg acgggcgggg gaaaatctct ttgctatcag ctgcccgcgc
     3001 tcaagatgcc gggactcacg ctcgttgtct cgccactcat cgcgctcatg aaagatcaag
     3061 ttgatgacct ccaagccaat ggcattgcgg ccgcgtttgt aaacagcact ttgagtccgg
     3121 cggagattga tcgaacacag gcggatgcgc gaacaggaaa acttaaaatt ttatacgctg
     3181 ctccggaaag attggcgaat cccggattcc aaaatttttt ggcggggctc aaaataagtt
     3241 tgattgccat tgacgaggcg cactgtatca gcgaatgggg tcatgatttt cgtcccgact
     3301 atcgaaattt gaaaatgctt cgaaatattt ttccaaatgt tccggtgatg gcgctcacag
     3361 ccactgccac cgaaaaggtg cgggaggata taattcgcca actgtcgctt gagaaagcaa
     3421 aaattttcat ttccagcttc aaccggccga atttgtccta cgcggtgttg cccaaaaaag
     3481 attcttatga tcagctgata gaaatattgc agggacacaa aaatgaatcg gtgatcgtct
     3541 attgtttttc tcgcaaagat acagaaaatc tggcgacgga cctgcgcaaa gaaggattca
     3601 aggctcttgc ctatcacgcg gggctggaga gcggcaaacg tcggaagaac caggaaaaat
     3661 tcatccggga tgaggcgcat atcatcgtgg cgacaatcgc gttcgggatg ggaatcgaca
     3721 agccggacgt gcgtttggtg gtgcactata gcttgccgaa atcaattgaa ggctactatc
     3781 aagaaactgg acgcgccggg cgcgatggtc tcccaagccg atgcattctt ttctattctt
     3841 acggtgacac ggtcaagcaa cagtttttca tccggcaaat cgaggacgag agtgaacggg
     3901 aaaatgccta caagaaactc aatcagatgg tcgagtattg cgaactggcc tcgtgccgcc
     3961 gatcacacct gctttcatat ttcggagaaa aatatgaagc ggaaaaatgt gacagttgcg
     4021 acgtctgtct ttcgccgaaa acggaatttg acgcgacggt gatcagtcaa aaaattcttt
     4081 cagccatggt tcggacgggt cagcggttcg gtgcaagcta tattgtcgac gttctcacag
     4141 gagcgaaaaa caaaaagctg ctggatcgcg gccatcaaaa attatcagtc tacggcatag
     4201 cgggcgattt ttcgaaagaa gagttgcgaa acatcatcgg ccaactgctt ggcaaaaaat
     4261 tgatcgtgaa aaccggggag gaatatccga ttctcgcttt gggagaacgc ggaaaagcgt
     4321 tcctgaaaaa tcgggaaaat attcttttgc agaagtctga aaccggccgt gaaacaattc
     4381 gaaagagcgc tgcgtttgag gcggattatg accagaaatt atttgataag ttgcgccttc
     4441 tgcgcaaaaa tatcgcggac gaaaaaggcg tgcctccgtt tgtcgtcttc ggcgatctgg
     4501 ccctcaccca aatggctacg tatttcccgc aaagcgaaga cagctttgcc aagatcagcg
     4561 gcgtgggcga ggagaaactg aagaaatacg gaaaaatatt cacggaggtc attcgggcgc
     4621 atgctaagaa aaatcatctc gctgaaaaaa gcattccggt gggacgatca cctcaagtgc
     4681 ggcgagcgat tcgaacagga tcgacgtatc aagaaactaa aaagttagcc cttcaaaaaa
     4741 tatccattga aaaaatggcc aaaacgcgcg gaatgacagt tggaaccatc acctctcatc
     4801 tcgagaagct gaccgcagct gaagaagaga tcgacattga atacctgcgt ccgcccgcca
     4861 ataaaatgga gaaaataaaa tccgcctttc gaaaatccgg cggcacggcc ttgtcgcccg
     4921 tccgcgaaat tctcggcgaa gagttttcat atgatgaatt gaaggtggcg aggatgtttc
     4981 tagacagaat agtgatataa tttcagataa taacattaaa aaaaccattc atatgaaaaa
     5041 agataaactt tcctaacatt aaatgttagg aagaaatttt gggaagtgag atggttacaa
     5101 aatgtgatcg gttgaaattg aaatcgaaag gaaatgagat ggttacagaa attaaccaac
     5161 tgaataaatt gaaaaaatga aaataatcct aaagataaac gaagaaaaaa acatcaaact
     5221 ctctcttttt gaggggaaaa gggaagttga cggtctggag tggaaggacg agaactcact
     5281 ttctcgcgtt ttactgtcca atttggacaa acttttgcaa aaaaacgggg tgggacttga
     5341 caagattgcc agctacaata taataagtaa agtgccgcag aaatggacga cttatcggat
     5401 cgcggatatt gccttgaaga cgctgaaaat cggaacttct gccagataag cttgccaaat
     5461 aattcacgct gtgatagact cgatattacg atatgtaact ttcgggggaa ttggaaaaat
     5521 catacaagaa taattaaata cttctaataa accacttagc caaaatcagc cgcttcccag
     5581 aaattttcga agcgctgatt ggagagtggc gtgtctttta tgtcgcaaga ggcgaaaact
     5641 ctcggggtga aagagtcttt tagcccgcag tcctctctca a
//
LOCUS       DATKGS010000056         4190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_163020, whole genome shotgun sequence.
ACCESSION   DATKGS010000056 DATKGS010000000
VERSION     DATKGS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163020"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VMQ48_01330"
     CDS             complement(<1..629)
                     /locus_tag="VMQ48_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUD08714.1"
                     /translation="MLKLYNTLTKSKEEFLPLHKGKVSIYTCGPTVYDYIHIGNIRAY
                     LTADILRRYLEYSGYEVRHIKNITDVGHLTDDDVAQGDTGEDKILKKALAEKKTPSEI
                     TKFFEESFRSDEKEMNILPAHYFPRATAHVPQMIKMIETLIEKGHAYEKNGNVFYDVT
                     SFKDYGKLSGNTLENLKVGARLEEHPDKKHPWDFALWLKADPNHILQWDS"
     gene            complement(648..1550)
                     /locus_tag="VMQ48_01335"
     CDS             complement(648..1550)
                     /locus_tag="VMQ48_01335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HUD08715.1"
                     /translation="MNNILELIGNTPIVRINKLNLNPNINIFAKLEGNNPGGSIKDRI
                     ALKMLEEAEKSGKLTREKTIIEATSGNTGIAIAMIAAVKEYFVEIVMSEAVSVERRKM
                     IAAFGAKITLTDGKLGTDGAIKKTRELVAEFPEKYYYPDQFSNNNNILAHYENTAAEI
                     WEQMDGKIDYFVAAIGTSGTLMGVSKYLKQKNPRVKVVSAEPVKGHYIQGLKNMEEAI
                     IPAIYDREHLDDIIMVESEAAFEMARLVARNEGIFCGMSSGAAMYASLEIAKKISAQG
                     GQAGDIVTIFPDRGEKYLSTSLFG"
     gene            complement(1848..2756)
                     /locus_tag="VMQ48_01340"
     CDS             complement(1848..2756)
                     /locus_tag="VMQ48_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HUD08716.1"
                     /translation="MRQIQTSKITWIDFSEPDADELLELKKKFKLHPLAIEEFTMPSA
                     RPKASLYGHCLYLTIHVPLFDKENRRTFPSELDIVMTDKYLITGHDQDIFQLRDFFQR
                     IQRSKKAQEQYMSQSPGHLLYYVIEDLLESCFPKIDHINENLDKIEEEIFKGNEKQMV
                     HEISYVKRDILNFRRTLKPQKSVLESLAKDNYPMIDTDLRSYYQDLVGTNIRVWSALE
                     NAKETIESLEGTNDALLSNKLNTTMKVLTIFSAVMLPMTVISSVLSMSMHIPLSQNTH
                     AFWIYSAGMLIISVITIVIFKVKKWI"
     gene            2863..3096
                     /gene="yidD"
                     /locus_tag="VMQ48_01345"
     CDS             2863..3096
                     /gene="yidD"
                     /locus_tag="VMQ48_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HUD08717.1"
                     /translation="MKSIFLKMIRWYQKYLSLDTGFLSYLYSERICRYHPTCSEYTYQ
                     AIEKYGVFLGGWIGLKRITRCHPWAKGGNDPLK"
     gene            3240..3983
                     /locus_tag="VMQ48_01350"
     CDS             3240..3983
                     /locus_tag="VMQ48_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08718.1"
                     /translation="MLTDRQKNLLSVVVKEYTQTAVPVGSKHIAKKYGFDLSSATIRA
                     EMNELEDAGYLFQPHTSAGRIPTDAGYRYFVEEIMPDKQLSKAEQKKMQAELLKLKAK
                     NTRLTRTTAKLLSSLSGNLAISGIKDEFDDFGMGELLDEPEFREMDDLCRLAETLDFI
                     DERVDQLVGKLKDNEVKIYIGKENPIKEISNCSMMVTPYKTKSGEKGILALIGPKRMK
                     YAKNKSLIEYMKKLLGGAVVVFYIAYLNF"
BASE COUNT         1105 a          858 c          976 g         1251 t
ORIGIN      
        1 gaatcccact gcaggatgtg gttcggatcg gctttcaacc acaaggcgaa atcccacgga
       61 tgctttttgt cagggtgctc ttcgagacgc gcgccgactt tcaaattttc gagcgtattg
      121 ccggaaagct ttccataatc cttgaaactt gtgacgtcat aaaaaacatt cccgtttttt
      181 tcgtaggcgt ggcctttttc gatcaacgtt tcgatcattt tgatcatctg cggaacgtga
      241 gctgttgccc ggggaaaata gtgagcagga aggatgttca tttctttttc gtcgcttcgg
      301 aacgactctt cgaaaaattt cgtgatctcg gagggagttt tcttttccgc cagtgctttt
      361 ttcagtatct tgtcttcacc cgtgtctcct tgcgcaacat catcatcagt caggtgtccc
      421 acgtctgtaa tgttctttat atgcctaacc tcgtaacctg aatattccaa atagcgccgc
      481 aggatatcag cggtgagata agcccggata ttgccgatat ggatataatc atataccgtc
      541 ggcccgcagg tataaattga aacttttccc ttgtgcagag gtaaaaattc ttcttttgat
      601 tttgtgaggg tattgtagag ctttagcata gttttatagt agcttattta tccaaaaagg
      661 gaagtgctca aatatttttc tccccgatcc gggaaaatag tgacgatgtc gccggcttgt
      721 ccgccttggg cggatatttt tttcgcaatc tccagactag catacatggc tgcgccggag
      781 gacataccgc aaaaaatgcc ttcattgcga gcgacaagtc ttgccatttc aaaagcggct
      841 tcgctttcaa ccataatgat gtcatcgaga tgctcgcggt catatatggc gggaatgatg
      901 gcttcttcca tatttttaag accttggata taatggccct tcactggttc ggcactcacg
      961 actttgacgc gcggattttt ttgcttgaga tatttcgata cgcccatcaa agttcccgaa
     1021 gttccgatgg cggcgacgaa atagtcgatt ttcccgtcca tttgctccca gatttcagcg
     1081 gcggtgttct catagtgagc aagaatattg ttattattcg aaaattggtc gggataataa
     1141 tatttttccg gaaactcggc gacgagctcg cgcgtttttt tgatcgcgcc gtccgttcct
     1201 agtttcccgt cagtgagtgt gattttcgca ccaaaggcgg cgatcatttt ccggcgttca
     1261 acagaaaccg cttcgctcat cacgatttcc acaaaatatt ctttcacggc ggcaatcatc
     1321 gcgatagcaa ttcccgtatt tcccgaagtg gcttcgataa ttgtcttttc tctcgtgagt
     1381 tttccggatt tttccgcttc ctcaagcatt ttgagtgcaa tccgatcctt gatgcttccg
     1441 ccggggttgt ttccttcgag tttggcaaaa atgttgatgt ttgggttcag attcagtttg
     1501 ttgatccgca caatcggtgt attgccgata agttcgagga tgttgttcat gattttttct
     1561 taatactaaa aaacttccca tcctattcgt aaatattcga aaaatattca ctcaaggacg
     1621 agaagtctaa ttcccgtggt tccacctcgg tcgtcgcaca atgcgacact tgattacagc
     1681 tattacgggc atacccgcgg ggttttactt gtttgtagac gtcggacgtc tacaaagttt
     1741 cttccccgag cctccccgat aaatcgggat aaacgaagcg cacgcttctt cgctaaagct
     1801 tttggaattg ttaagtgatt ttatgttaag cgtattttgc tgaaaggtca aatccacttc
     1861 ttcaccttga aaatcacgat cgtaatcaca gaaatgatga gcattccggc agaataaatc
     1921 caaaaagcgt gagtattttg cgaaagggga atatgcatac tcatcgaaag gacgctcgaa
     1981 ataacggtca ttgggagcat gacagcggaa aatatcgtga gaaccttcat tgtcgtgttc
     2041 aatttgttcg aaagcagggc gtcgtttgtt ccttcgagcg attcgatcgt ttctttggcg
     2101 ttttcgagag cagaccagac acggatgttc gtgccaacaa ggtcttgata atatgaacgc
     2161 agatcggtgt cgatcatggg ataattgtct ttcgcgagtg attccaagac cgatttttga
     2221 ggtttcaaag tgcggcggaa attcagaata tcgcgcttga cgtaggaaat ctcatggacc
     2281 atctgttttt cattgccttt gaaaatttct tcttcaattt tatcaagatt ttcgttgatg
     2341 tggtctattt tcgggaaaca agattcgaga agatcttcaa tgacgtagta gagcaaatgg
     2401 ccgggggact ggctcatata ttgctcctgc gcttttttgc tccgttggat cctttggaaa
     2461 aaatcgcgaa gctggaaaat atcctggtcg tgtccggtaa tgaggtattt gtctgtcatg
     2521 actatgtcca gctcgctggg aaaggtgcgg cggttttcct tgtcaaaaag aggaacgtgg
     2581 atcgtgagat agagacaatg gccatagagg ctggctttcg gcctcgcgga aggcatcgta
     2641 aactcttcga tcgccagagg atgaagtttg aattttttct tgagttccaa aagctcgtcc
     2701 gcgtccggtt cggaaaaatc gatccaggtt atttttgatg tttgtatttg gcgcataaaa
     2761 actcaaaacg caaaactcaa aacgcaaaac cacagttcaa aattcaaaac tttttgcgtt
     2821 ttttgtttgg tttttatttt ttagttctgc taatattata ccatgaagtc aattttcttg
     2881 aaaatgatcc gctggtacca gaaatatctg tccctagata cgggtttttt gtcgtacctc
     2941 tattccgagc ggatttgccg ctatcatccg acttgcagcg agtacaccta tcaagcgatt
     3001 gaaaaatacg gtgtatttct aggtggatgg ataggtctca agcgaataac gagatgtcat
     3061 ccttgggcga agggcgggaa tgaccccttg aaatgaaaga cttgaccccg caatatttcg
     3121 ctttgctaaa taggcgggac aggcagctat tttgaaatat ttgacagttt atgcgggtat
     3181 tatataattg attggcactc acccttgacg agtgccaatt ggagcgatat aatgatctta
     3241 tgctcacaga tcgtcagaaa aatttgcttt ccgtcgtagt gaaagaatat acccaaacgg
     3301 ccgtgccggt cggatcaaaa catattgcca aaaaatatgg ttttgatttg agttcggcga
     3361 caattcgggc ggaaatgaac gaacttgaag acgcgggcta tctttttcag ccgcacacga
     3421 gcgcaggaag gattccgacg gacgcgggat atcgctattt tgtcgaggag atcatgcccg
     3481 ataagcagct ttcgaaagcc gaacagaaaa agatgcaggc ggagcttttg aagctcaaag
     3541 cgaaaaatac ccggcttacg cgtacgacgg caaaattatt gtcgagtttg agtggaaatc
     3601 tggccatcag tgggatcaaa gacgagtttg acgattttgg gatgggcgag cttctcgacg
     3661 agccggaatt tcgggaaatg gatgatctct gccgtcttgc agaaacgctt gatttcattg
     3721 atgagcgggt ggatcaactg gtcggaaagc tgaaagacaa cgaagtgaaa atatatattg
     3781 gcaaagaaaa tccgatcaag gaaatttcca attgctcaat gatggtgacg ccgtacaaga
     3841 ccaaatccgg tgaaaaagga atccttgccc tcatcggccc gaaacggatg aaatacgcga
     3901 aaaacaaatc attgattgag tatatgaaga agcttttggg tggagcggtg gtggtatttt
     3961 atatagcgta tttgaatttt taaggtttag ccttaaaaat gtatctagtt ttatttttaa
     4021 gatcggatct taaaaaagga aattttatgc aaaacgaaga aaaaaagttc cacagtcgga
     4081 tttcacaaaa gatttttctg tataataagg aaaagaattc ttttttgctc gtaaagatga
     4141 gaaatgatca ggcatatttt gcaaagaagt atggtgtttc ttctccctct
//
LOCUS       DATKGS010000057        13281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_105768, whole genome shotgun sequence.
ACCESSION   DATKGS010000057 DATKGS010000000
VERSION     DATKGS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105768"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VMQ48_01355"
     CDS             <1..279
                     /locus_tag="VMQ48_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUD08719.1"
                     /translation="SVAAGFLIDKMIINEKIGALSEGQKGLVAFARLVLEEPGLLILD
                     EPTNHINFRHLPVIAEALDSYKGAMVLVSHVPEFVDQIRIDETLDLEK"
     gene            254..844
                     /locus_tag="VMQ48_01360"
     CDS             254..844
                     /locus_tag="VMQ48_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HUD08720.1"
                     /translation="MKRWIWKSSDYVFLGGFHLTKFAKYRILSIISSIKKKYMELENK
                     IKEIIFGLFGSIIASFGVSLGASFLTSDHLTILLAGLIVGIASSFANAFGPLVSSSQM
                     LTRQTYSKQDFQQAVGSSVLTFVIVGLPLVSYVLISDLGIARIISVITGLVLLFIFGV
                     HQAQLERKSALGYGFSMALVGALFVGLFYFLAVLFK"
     gene            1022..2062
                     /locus_tag="VMQ48_01365"
     CDS             1022..2062
                     /locus_tag="VMQ48_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUD08721.1"
                     /translation="MKTNFNTYFFYAILIGLTVLAFFIIKPFLIPFLFALILVHLFNP
                     VYNFLLEKTERKWLSSLLTCLLIALVIMIPVLIISSLLVGEVQSAIINLASNPESLKK
                     ITNIAQNLSSLPIFKSLELGKILNPDSILSALSSFSQGFLFILEGTYAGILHFIFVMI
                     MIFFSLFYMFFDGKKLLDKIVKILPLQDKYDSILRSDINSMIRATIKGIMLMAIFEGI
                     VGGVLFWSAGVAAPILFGLLMAVFSVIPPFGAGLVWLPVGIIMMLLGYPTQGIIILLT
                     GVLVIGAMDNFLRPKLIGKDTQLHPLFILFSTLGGIIFFGISGFIIGPIIVSLLVALW
                     DIYVLDFKTQSD"
     gene            complement(2088..3095)
                     /locus_tag="VMQ48_01370"
     CDS             complement(2088..3095)
                     /locus_tag="VMQ48_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08722.1"
                     /translation="MWLLIAIIIGDAAFMVDQYRSSHADQSTAGTEQKAAPVAEVKKP
                     EPIKAQAVDNPEYQPVRDEKVTDLAIPTAHAFVVVDADTGKILSGNHETDQRQIASLT
                     KMMTAILVMEKIKNLDEPVNIGDEEVYIEGTKIGCPRSGYCISPRLKIGEKVSAKSLL
                     QAMLMNSANDAAIALGKHISGSQDSFASLMNEKAKQMGLINTHFCTPSGLEPDGRESE
                     CYSSASDIARISAYSMRFDTVWKMFGYPNNSQITSCDGQVAHTLINSDILLNDIPNCL
                     GGKTGFTPAAGYSLLMGVSDPTHLHRIIVVVLDDPYRWQDIRTAINWTFQAYSWEKQK
                     F"
     gene            3276..3971
                     /locus_tag="VMQ48_01375"
     CDS             3276..3971
                     /locus_tag="VMQ48_01375"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HUD08723.1"
                     /translation="MEKQKEKNKTHTLIISDLHLGSRVSRAKEVAEFLKNFEFKKLIL
                     LGDIFEDLNFNRLRNDDWKFLALLTKFSKTRKVRWVVGNHDKDLAKIFAVFTGAKVYS
                     VYKWRYGRKKFLAIHGHQFDNFLTDNPLLSLIAPQIYNFIQLVDFSDKRISRYIKRQS
                     KLWMRNSEKVAARAFLYARLRGIDYIFCGHTHKALQERRGKIRYFNSGCWTEIPSTYI
                     TIDGKKIEICKYQ"
     gene            complement(4110..5369)
                     /locus_tag="VMQ48_01380"
     CDS             complement(4110..5369)
                     /locus_tag="VMQ48_01380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUD08724.1"
                     /translation="MRNTNSRYSSGRRSFSPSRVRSNQSGAKGRSFGGGRRPAGRSQS
                     RGGGRFALSPLDLSAFINKAAVITEKVEHFVPEHQFPDFKLNPTILQTLTRAGYQQPT
                     PIQDKIIPHILNDRDVVGLANTGTGKTAAFLIPLIHKQIGDKKKQVLILTPTRELALQ
                     IDQEFRIFSKGLGLYSVVCVGGANINMQIRDLRYFNNLIIGTPGRVIDLINRRVLKLG
                     EINTVVLDEADQMLDMGFVNDIKLIMSNVPAERHTLCFAATMSATIEGLISKFLRDPI
                     TISVKTQATSKNVQQDIVKMHGADKIDKLHDILNDPEFKKVLVFGRTKHGVENLSKKL
                     ITRGFKAESIHGNKSQGQRQRALKKFKDNQVQVLVATDVAARGLDIDSVSHVINYDLP
                     ATYDDYIHRIGRTGRGSKKGKALTFIE"
     gene            5588..6208
                     /locus_tag="VMQ48_01385"
     CDS             5588..6208
                     /locus_tag="VMQ48_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUD08725.1"
                     /translation="MLWHLLVIALTYVFVVSGFDWFYFQATRSAALQVFFWPAVLAGA
                     FIPLFGILLFYLISSAGKNAKLAGTALALGQAALLGLLVSDFYKFFTGRPGPPDVFGS
                     VAAIDMTHIFHFGILRGGVFFGWPSSHTTVAFAMAVTLATLFPKNKLVKLIALVYAFY
                     VGIGVSMTIHWFSDFAAGAIFGSIVGIVVGKSYRDRNSFSLGRNKK"
     gene            6221..7351
                     /locus_tag="VMQ48_01390"
     CDS             6221..7351
                     /locus_tag="VMQ48_01390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08726.1"
                     /translation="METAFKKYKLILGVFLIWLVLLLLVGHFSQSLSKWPGLKSCGNK
                     FDPAYYRWDSGWYTEISQKGYSYSPDRQSSVAFWPLYPLSIRAAHVITNLRPGKVAYD
                     LSILFAFLATIVIYRLARLDYPEKTSQYAIFFILFWPASYFLVAVYPESLFVFLAALS
                     LYLGRKKKWLWAGIVAALLALTKPYGVFMAPTLMAEYFIVSGKDWKIFFRKRDWWPLL
                     LPIISVVSFAYYNYHKFGNAFAFLQTEKNWGRGYGNFFLSLAGEARDFMVPPTKLLDG
                     SHSQYIIYLVSFFFSIWAFRVSWKKVRRTYLIFPLLILAAALTSGTLTSWSRYMLLGF
                     PILFGPAVWLAEKKYLKYAWLFVSLALLLFIANLFVRCYPVE"
     gene            7407..8483
                     /locus_tag="VMQ48_01395"
     CDS             7407..8483
                     /locus_tag="VMQ48_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUD08727.1"
                     /translation="MDKMKKYLLIGGAAVLVLAIAVFGFFLLRAGKTSSTTSPQVATQ
                     PSVPVPNSFRGTLADVADRIANIVSPPEASTPPSPFTNKVPGSSSSGDGTSFSFAIFG
                     DTKEFSANDPNGNLQKAVAQIKGMDLKAVFVMGDLIKSCGDGCAQKFNDWKSVMAPVL
                     SKTYEVVGNHDRTGGDSDDAVWQKAFNLPQNGPSGYKDLVYSFDIGNSHFAVLDSEKP
                     RTTIINSVQRDWLDKDLTASNKEHKFVFFHEPAFQVAQDAEDGLDAHPTERDALWNII
                     KKDKVDAVFNGHAHIYARKKIDNVEQIVVGDTDSTNDDTVQAGLSDFGYKGKSYAIVT
                     VNGKNINFKLFTVDGKQIDSFDFL"
     gene            8586..9776
                     /locus_tag="VMQ48_01400"
     CDS             8586..9776
                     /locus_tag="VMQ48_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08728.1"
                     /translation="MDRETAYRRKKIYLWAAGLVLAGMALGGFWLFEKNNAGSGEGAF
                     QPAAEAQALPSGQTLDSFAGVLNPSKPPPPPVDMNRLKTQGCVTDGLLTEYNPDQGEY
                     IQLINRSNCYYLHRAIETWLAPPDFTVIEKNMAQVAKPDVVYGMFIAEAIRFKDDYRN
                     EDTGHVFDFRKMCHDNTMNDWGEHTCKPTFSSKEYRDYLSYITHKAIDLGVQSFTFGQ
                     IYLQESSSRDYAPQIMKDIRQYAKKKNVDVVIGAQTGSITDAKYLKLFDYIEGGVGIN
                     SNGDIENGPCLSTRGSCWALLWNQVYASKAKNVLLNLDWTGIPSDDLDIFARMNAGKR
                     AATLKNLYGFFKQKNMGFLMPYFGVLDTGNGGCFGPKKGFYSPSDAYSCRDEGTISKI
                     LGGS"
     gene            9902..10102
                     /locus_tag="VMQ48_01405"
     CDS             9902..10102
                     /locus_tag="VMQ48_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HUD08729.1"
                     /translation="MAQGTIKRLTDRGFGFIAQEGSDKDLFFHSSELVGVDFNDLKEG
                     DNVTFEIGESPKGPNATKVEKA"
     gene            10216..10389
                     /locus_tag="VMQ48_01410"
     CDS             10216..10389
                     /locus_tag="VMQ48_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08730.1"
                     /translation="MSKTFIMIGLFLGSIAGGYIPLLWGGSVFSISSILLSGAGALVG
                     IWLGFRVAQRLDI"
     gene            10420..11625
                     /locus_tag="VMQ48_01415"
     CDS             10420..11625
                     /locus_tag="VMQ48_01415"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HUD08731.1"
                     /translation="MENTAMQKEKIKKIAYTSLIIAFFITTSGGFSFSQNVAEAKKSS
                     VTKWQLKLQKSGVDTALWQKYLPDINRKDYIKITKLAASSPATNTGTETTATAQPAAT
                     TSGSLGPEISVGLRYNSKSDTFKIDANKTYNIRDGGENGKVIAQIAGGSTTKVDYDGN
                     GNLKVSGSVADTSVSDQVWFDAADGDNSTIIFNTHGSDSYDHYRGKIEVNYYHGDDIY
                     GGNSNTVTQIWLINKLPLEQYVWGMGETTGTGDTDHVRVMTTIFRTYGDWYIENATKY
                     KPLGFKIRSDSNSQIYGGYDWEKDHPHIKEAANDTRGKIVKYKGDVALTPYCSYTDGK
                     TRSMDGYPYLKSVKDNKAGIKKGLKPGDGGNHMWGLSAHGALGFAQDGKSWDWILKYY
                     YTKVDISSL"
     gene            11690..12217
                     /locus_tag="VMQ48_01420"
     CDS             11690..12217
                     /locus_tag="VMQ48_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08732.1"
                     /translation="MSNGMKKSKIIAIIIFIIAAAAVLYFAYGVVKKRYMSPAKNAGT
                     SSSATQNQAGSQTDNQNVDQSGDNQVSTPSEVDNTAPTNGQPDVQSADCDNNCAQYKD
                     NADNLKYCQEVCGITPVKPKNSEEDCANLSGLDKDSCWRDLAVSKKDFAICDKISDAK
                     LQKVCHNRVTEEVLN"
     gene            complement(12214..13020)
                     /locus_tag="VMQ48_01425"
     CDS             complement(12214..13020)
                     /locus_tag="VMQ48_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUD08733.1"
                     /translation="MSLSLPEIKDSITSGRTKGANINNPKKKPTDSDGHGTFVAGIIA
                     AKTNNKKGIAGASFFGNLKIMPLKFDFTTDQAISAVSYAKARNIPIINASWGAYGEEG
                     LDLVLKDTIASYPGVFVTAAGNGDSKTYIGYNHDGTDPNKKMYPCDFDLANIICVGAS
                     DKNGALAEYSDYGATSVDVAAPGGTDDNPIFGLDLKKNRYVEEEGSSLSTAYVSAEAG
                     LILSKKPNLSSTQIIGIIKNSVDPEPSLAGKTVTGGKINFQKALQMAGSY"
BASE COUNT         3291 a         3008 c         3315 g         3667 t
ORIGIN      
        1 tcggtcgcgg ccggattttt gatcgacaaa atgatcatca acgaaaaaat cggcgcgctt
       61 tctgaaggac aaaagggtct cgttgctttt gcgcgccttg tgcttgaaga accggggctt
      121 ctcatcttgg acgagcccac caatcatatc aacttccgcc atctgccggt gattgccgag
      181 gccttggaca gctacaaagg cgccatggtt ctggtcagcc acgtgccgga attcgtcgac
      241 caaatccgga tcgatgaaac gttggatttg gaaaagtagc gactatgttt ttcttggcgg
      301 attccatttg acaaaatttg ccaaatatcg catactatca ataatttcaa gcattaagaa
      361 aaaatatatg gagctggaaa ataagatcaa ggaaataatc tttggcttgt tcggatccat
      421 tatcgccagc tttggagtgt ctttgggcgc ttcttttttg acctccgacc atttgaccat
      481 attactggcc ggtttgattg taggaatcgc cagcagtttt gccaatgctt tcggcccgtt
      541 ggtttcaagt tcccaaatgc ttacccgcca aacctattcc aagcaggatt ttcaacaagc
      601 cgtcggatct tctgtgctta cttttgtcat tgtcgggctg ccgcttgttt cctacgtcct
      661 gatcagcgat ctgggaattg ccagaataat ctcagtgata accggcttgg ttcttctttt
      721 cattttcggt gtccaccaag ctcaattgga acgcaaatct gcgcttgggt atggcttctc
      781 aatggctttg gttggcgctc ttttcgtggg gctcttttac tttctggctg ttttgttcaa
      841 ataaaggcgg ttattctgga gaatacgatt cattccccga aatacaaggt tctggctgca
      901 atatccttat ggctggttcg gttgcaggcc attttttcag acttaaataa agcctgattt
      961 ttgttgccca agagggacga tggaaattgt gatataatga gatcatcaac aaatgcaaaa
     1021 tatgaaaacg aatttcaaca cgtatttttt ctacgccatc ctaatcggac taacggtttt
     1081 ggcttttttc ataataaagc cttttctgat ccctttttta ttcgccttga ttctggttca
     1141 tctgttcaat cccgtttaca attttttgct agaaaaaact gagaggaagt ggctaagctc
     1201 gctgctgacg tgccttctta tcgctctcgt catcatgatt cccgttctaa taatttcatc
     1261 cttgctagtg ggagaagttc agtctgctat catcaatctt gcctcaaatc ctgagtcgtt
     1321 gaaaaaaata accaatattg cccagaactt gtcgtctctt cctattttta aatcgcttga
     1381 gcttggaaaa atattaaatc cagattccat actgtcagca ttgagcagct tttcacaggg
     1441 attcttattc atcctcgagg gaacgtatgc aggaatcttg cattttatct tcgtaatgat
     1501 aatgatattc ttctcgcttt tttatatgtt tttcgatggg aaaaaacttc ttgataaaat
     1561 agtgaaaatt cttcctctgc aggacaaata tgacagtatt ttgcggagcg atataaactc
     1621 aatgattcga gctacgatca agggaataat gcttatggcg attttcgagg gaatcgttgg
     1681 cggcgttctt ttttggtcgg ctggtgtggc agctcccatc ttattcggtc ttttgatggc
     1741 tgttttttcc gttattccac cctttggagc gggactggta tggcttccag tcgggataat
     1801 aatgatgtta ttgggctacc cgacacaagg tataattatt ctcttgacag gtgtcctggt
     1861 tataggtgcg atggataatt ttcttcgccc gaagctaatc ggaaaagaca ctcaactgca
     1921 tccacttttt attctgtttt ccacgctggg aggaataatc tttttcggta tttccggatt
     1981 tataataggt ccaataatcg tgtccctctt ggttgcctta tgggatattt atgttttaga
     2041 tttcaagacg caatcggatt agtattgcct tctttcatcc ataaatttta aaatttttgc
     2101 ttttcccacg aataagcttg gaaagtccaa ttgatggctg tgcggatgtc ttgccagcga
     2161 tagggatcgt caaggacgac gacgatgatg cggtggagat gggtcggatc cgacactccc
     2221 atgagaagag aatatccagc cgctggagtg aaaccggttt tgccgccgag gcagttggga
     2281 atatcattga gaagaatatc gctgttgatg agcgtatgcg cgacctgccc gtcgcaggaa
     2341 gtaatttgcg agttgttcgg atagccgaac attttccaaa ctgtgtcgaa gcgcatcgaa
     2401 taggcggaga tgcgggcgat gtcggatgcg gaggaatagc attccgattc gcgtccatcc
     2461 ggctcgagcc cggaaggcgt gcaaaaatgc gtgttgatca gccccatttg tttcgctttc
     2521 tcattcatga gactcgcgaa agaatcttgc gatccggaga tatgttttcc gagggcgatg
     2581 gccgcgtcgt tggcgctgtt catgagcatt gcctggagga gacttttggc cgaaactttt
     2641 tctccgattt tgagccgcgg actgatgcaa tagccgctgc gcgggcagcc aatctttgtt
     2701 ccttcgatat agacttcctc atcgccaata ttgaccggtt cgtcgagatt cttgattttt
     2761 tccatgacca ggatcgctgt catcattttt gtgagcgagg cgatctgcct ttggtccgtt
     2821 tcatggttgc cggataatat ttttccggtg tcggcgtcaa ccaccacaaa agcgtgggca
     2881 gtcgggatcg cgaggtctgt cactttttca tcgcgcaccg gctgatattc ggggttgtca
     2941 acggcttgag ctttgatcgg ttccggtttt ttcacttctg ccaccggcgc ggctttttgt
     3001 tcagtgccag ccgtgctctg atcagcgtgc gaactgcgat attgatcaac cataaacgct
     3061 gcgtctccga tgatgatcgc gatgagcaac cacaaaagaa aaccaggtct tggttttcga
     3121 ggaggtggag cgggcttgaa tctttggggt gtgtaaatta tgtctctcac gaagaaattt
     3181 tagcaaatgt atcccgctgg cgcaaatcct cggagggcaa gtatttttat gcgtaatgca
     3241 tccatcgccg ctgtatggta aaataaaacc aaccgatgga aaaacaaaaa gagaaaaaca
     3301 aaacccatac cctcatcatt tccgatctgc atctgggatc gcgggtgagt cgcgccaaag
     3361 aggtcgctga attcttgaaa aacttcgagt tcaagaaatt gattttgctt ggagatattt
     3421 ttgaagatct caatttcaac cgtcttcgaa atgatgattg gaaattcctc gcgcttctca
     3481 cgaagttttc caaaaccaga aaggtgcgct gggttgtggg aaaccacgac aaagaccttg
     3541 cgaaaatttt tgccgtgttc accggcgcga aagtatacag tgtctataaa tggcggtatg
     3601 ggcgcaaaaa atttctggct attcacgggc accagtttga taacttcctg accgacaatc
     3661 cattgttgag cttgatcgct cctcaaatat acaatttcat ccaactggtt gatttttccg
     3721 acaaaaggat tagccgttac atcaaaaggc aaagcaagct ttggatgcgc aattcagaaa
     3781 aagtcgcggc gcgcgctttc ctctatgcgc ggttgagggg aatcgactat attttctgcg
     3841 gacacacgca caaagccttg caagagcgaa gggggaaaat ccgttatttc aactccggct
     3901 gttggacgga aattccttca acctatatca ccatcgacgg caaaaaaatc gagatttgca
     3961 aatatcagtg atgatgtatt ccctaatatt aaatgtcagg agcggttaaa atccggttct
     4021 aaccgtgtga actagccagc tggtgagaac cttattttga atcaaaacag ccccaatatt
     4081 ttggagctgt ttttctattt caaaatatct tattcaataa acgtcagcgc tttgcctttc
     4141 ttggatccgc ggccggtacg gccaattcgg tggatgtagt cgtcgtaggt ggcgggaagg
     4201 tcataattga tgacatggct gacgctgtcg atatcgagtc cgcgggcggc gacgtccgtc
     4261 gcaaccagaa cctgaacctg attgtctttg aattttttga gagcgcgctg gcgctggcct
     4321 tgcgatttgt tgccgtgaat ggattcggct ttgaagccgc gggtgatgag tttttttgaa
     4381 agattttcaa cgccgtgctt ggtccggccg aagaccagca cctttttgaa ttcggggtcg
     4441 ttcaagatgt catgaagttt gtcgatcttg tccgcgccgt gcatcttgac gatatcctgc
     4501 tggacgtttt ttgaagtggc ctgggttttc actgaaatag tgatcgggtc gcgcagaaat
     4561 ttgctgatca agccttcgat ggtggccgac atcgtggctg cgaaacacag cgtgtgcctc
     4621 tcagctggaa cgttcgacat gatcagtttg atgtcattca caaatcccat atcaagcatc
     4681 tggtcggctt catcgagcac gacggtattg atctcgccga gcttcaaaac tcttcgattg
     4741 atcaaatcga tgactcttcc aggcgtgccg ataatcaggt tgttgaagta gcggagatca
     4801 cggatctgca tgttgatatt ggcgccgccg acacaaacaa cggaatagag gccgaggcct
     4861 ttcgagaaaa ttcggaattc ctggtcaatc tggagggcca gttcgcgggt cggggtcagg
     4921 ataagaacct gctttttctt gtcgccgatt tgtttgtgaa tcagagggat gagaaaggcg
     4981 gctgtttttc cggtgccggt gttggcaaga cccacgacgt cccggtcatt caaaatatgg
     5041 gggatgatct tgtcttggat tggagttggc tgctggtagc cggctcttgt gagagtctgg
     5101 agaattgtcg gattgagctt gaagtcggga aattggtgtt ccgggacaaa atgctccact
     5161 ttttcggtga taaccgcggc tttgttgatg aaagccgaaa ggtcgagcgg ggacagcgcg
     5221 aaccggccgc ctcctcgtga ttggctccgg ccggccggcc gtcggccacc accaaaagaa
     5281 cgcccttttg cgcctgattg gttggaccga acgcgactcg gtgaaaagct tcggcgtccg
     5341 gatgaatatc ttgaattggt gtttctcatg ttgtttttga ataataaaag gctgcttttt
     5401 gaacagtctg ttaattatat accgattata tatatttgta aagggctgcg tcgaaaaata
     5461 ttgtttttgc taggcctttc tcttctttga gggtataata ggatcattga ttgtatattt
     5521 ttttcacatg cgcggatttt tccataatat tctgaagaat ataaaaaaca ttttttgggg
     5581 gcgcaatttg ctctggcatc ttttggtgat cgcactgaca tatgtttttg tcgtgtccgg
     5641 atttgattgg ttttattttc aagcaacgcg ctctgccgcc ctgcaagttt ttttctggcc
     5701 ggcggttctc gcaggggctt tcattccgct tttcggaatt ttgctttttt atctcatcag
     5761 ttcggctgga aaaaatgcca agctggcggg tactgctctt gcgctcggtc aggcggcgct
     5821 tttgggactt ttggtttccg atttttacaa atttttcacc ggccggccgg gtccgccgga
     5881 tgttttcggg agcgtagctg ctatcgatat gacccacatt ttccatttcg ggattttgcg
     5941 cgggggagta tttttcggtt ggccgtcttc gcacacgacg gttgcgttcg cgatggcggt
     6001 gacactcgct acgctttttc caaaaaacaa attagtgaaa cttattgcgc tggtctatgc
     6061 tttttatgtc ggtatcggcg tgtcgatgac tatccactgg ttttctgatt ttgcggccgg
     6121 ggcgatcttc gggagcatcg tcggaattgt tgtgggaaaa agctatcgcg accgaaattc
     6181 tttttcgctg ggccgaaaca aaaaatgata gaataaaatc atggagactg cgttcaaaaa
     6241 atacaaattg atcctggggg tatttttgat ctggctggtc ttgcttcttc tcgttggaca
     6301 tttcagccaa tctctttcca aatggccggg tctcaagagc tgcggaaaca aattcgatcc
     6361 cgcctattat cgctgggatt cgggctggta tacggaaatt tcccaaaaag gatactccta
     6421 ttcacccgac cggcaatcga gcgtcgcttt ctggccgctc tatccgcttt ctatccgcgc
     6481 ggcccatgtc atcaccaacc ttcgtccggg aaaagtggca tatgatctga gcatcctttt
     6541 tgcttttctt gctacaattg ttatctatag attggcgcgt cttgattatc cggaaaaaac
     6601 cagccagtat gccatctttt tcatcctgtt ttggccggcc tcatattttt tggtcgccgt
     6661 ctatcccgaa tcactttttg tctttctggc cgcgttgagt ttgtacctgg gaaggaagaa
     6721 aaaatggctt tgggcgggaa ttgttgcggc tctcctcgcg ctcacgaaac cgtatggagt
     6781 tttcatggcg ccaactctca tggcggaata tttcatagtc agcgggaaag actggaaaat
     6841 atttttccgg aaacgggatt ggtggccgct tcttttgccg atcatttctg ttgtttcttt
     6901 tgcgtactat aactatcaca aatttggaaa tgcttttgct tttcttcaga cggaaaaaaa
     6961 ttggggcagg ggctatggca atttttttct ttccttggcg ggggaggcca gggatttcat
     7021 ggttccgccg acaaaacttt tggacggttc tcattcccaa tacatcatct atcttgtcag
     7081 ttttttcttt tccatctggg cttttcgggt gagctggaaa aaagtgcgcc ggacctatct
     7141 cattttcccg ctgctcatcc tggcggccgc gctcacctcc gggacgctca ccagctggtc
     7201 gcggtatatg ctgctcggct ttccgatcct tttcgggccg gcagtttggc tcgcggaaaa
     7261 aaaatatctg aaatatgcgt ggctctttgt ttcgctcgcg ctcttgcttt tcattgccaa
     7321 ccttttcgtg cgctgctatc cggtggaata ggcaattttg aaaatattgg ctgattttgc
     7381 gtattaaatg atatagataa aaatatatgg acaagatgaa aaaatatttg ctcatcgggg
     7441 gtgcagccgt gctggtgctt gcaattgccg tgttcggctt ctttcttttg cgggccggga
     7501 aaacgagttc aacaacatcg ccgcaagtgg cgacccagcc atccgtgccg gtgcccaatt
     7561 cttttcgggg gacgctggcg gatgtggcag acaggatagc gaatattgtt tctccccctg
     7621 aagcttcgac accgccttcg ccattcacca acaaagtacc cggcagctcc agctcgggag
     7681 atggaacaag tttttctttc gccatattcg gcgacacgaa agaattcagc gcgaacgatc
     7741 cgaacggaaa tcttcaaaaa gcggtggcgc aaataaaagg tatggatctc aaagctgttt
     7801 ttgtgatggg cgatctcatc aaaagctgcg gagacggctg cgcgcaaaaa ttcaatgatt
     7861 ggaaaagcgt catggcgccg gtcctttcaa aaacatatga agtcgttgga aaccacgacc
     7921 ggacgggagg cgattccgac gacgcagttt ggcaaaaggc cttcaatctt ccgcagaacg
     7981 gtccgagcgg atacaaagat ctggtttatt cattcgacat cggcaattct catttcgccg
     8041 tgctcgacag tgaaaagccg cgtacgacca tcatcaattc cgtccagcgc gattggctgg
     8101 ataaggatct aacagcttca aacaaagagc ataaatttgt gttcttccat gagccggctt
     8161 tccaagtggc gcaagatgcg gaggacggtc tcgatgccca cccgaccgag cgagacgccc
     8221 tttggaatat catcaaaaaa gacaaagtcg atgcggtgtt caacggccac gcccacatct
     8281 atgcccggaa aaaaatagac aacgtcgagc agatcgtggt cggggatact gacagcacga
     8341 atgatgacac tgtccaggcc ggcctttccg atttcggcta caaagggaaa tcatacgcga
     8401 ttgtgacggt caacggaaaa aatatcaatt tcaaactttt caccgtggac ggaaaacaaa
     8461 tcgattcgtt tgattttctt tgagaaacat tttccaactg acccttgaga aagggttttt
     8521 tggttttgta aaaatatggg caagacaaat tttcccggat gcgtaataat ggacagacaa
     8581 gcattatgga tagagaaacg gcttatcgcc gcaaaaaaat atatctctgg gcagcaggtt
     8641 tggttttggc gggcatggcg ctcggcggtt tttggctgtt tgagaaaaac aatgccggtt
     8701 ccggcgaggg cgctttccag ccggctgccg aagcacaagc cttaccgtcc gggcaaacgc
     8761 ttgattcttt tgccggagtt ctcaatccat cgaagcctcc gcctccgccg gtcgatatga
     8821 accggctcaa gacgcagggc tgcgtgacgg acgggctgct cacggaatac aatcccgacc
     8881 agggggaata tatccagctc atcaaccgtt cgaattgcta ctatctccac cgggcgatcg
     8941 agacgtggct cgcgccgccc gacttcacgg tgatcgaaaa gaatatggcg caagtcgcaa
     9001 aaccggatgt cgtctacgga atgttcatcg ccgaagccat ccgcttcaag gatgattaca
     9061 gaaacgaaga tacgggccac gtttttgact ttcggaaaat gtgccatgac aacacgatga
     9121 atgattgggg agagcatacc tgcaagccaa ccttttcttc aaaagaatat cgggactatc
     9181 tttcctatat cactcacaaa gcgatcgacc ttggcgtgca gagtttcacg ttcgggcaaa
     9241 tatacctgca ggaaagttcg agtcgggact acgctccgca aattatgaag gacatccgcc
     9301 agtatgccaa gaaaaaaaat gtcgacgttg tgatcggcgc gcagacaggc tccatcactg
     9361 acgcgaaata tctcaaactc tttgactaca ttgaaggggg agtaggcatc aattccaacg
     9421 gtgacattga aaacggccct tgtctctcga cgagaggcag ctgctgggcg cttctttgga
     9481 accaagtcta cgcctcgaaa gccaagaatg tgcttctgaa ccttgattgg accgggatcc
     9541 cgtcagatga tctggatatc ttcgcgcgga tgaatgcggg aaaacgggca gcgacgctca
     9601 aaaacctcta cggctttttc aaacagaaaa atatgggctt tcttatgcca tatttcggcg
     9661 tgctcgacac gggcaacggc ggttgtttcg gccccaagaa aggattttat tctccttccg
     9721 acgcctattc ttgccgggat gaaggcacaa tcagcaagat tttgggcggt tcttgagtca
     9781 tagttgacaa aatgggcaga agtgctatac ttatcacatc acaagagtat gcaatagcag
     9841 cgtcgtcgaa ctcatttttg cattacattc aaatgtgaat ttaaataatc tccaaagaaa
     9901 aatggcacaa ggaacaatca agaggctcac agaccgggga ttcggattca ttgcccaaga
     9961 aggcagtgac aaggatctct tcttccactc gagcgagctc gtcggagttg acttcaacga
    10021 cctcaaggaa ggcgacaatg tgacgttcga aatcggcgaa agcccgaaag gaccgaacgc
    10081 gaccaaagtc gaaaaggcct agtcagaact catacttctg aaaccaaaca gccctgatgc
    10141 aaatcggggt tgttttttta ggatagaaaa aatgggcagc ttgtgataaa atatttttat
    10201 aagacaaaaa gcgctatgtc caaaactttc atcatgatcg gattgtttct cggatcgatt
    10261 gcgggcgggt atatcccact cctttgggga ggaagtgttt tttcgatttc ttcgatactt
    10321 ctgagcggag ccggggcgct tgtcggaatt tggctcgggt tcagagtcgc ccagcggctg
    10381 gatatttgag aatggacttt ttcttgaaaa tgtgctaaaa tggaaaatac tgcaatgcaa
    10441 aaagaaaaaa tcaaaaaaat cgcatataca tccctcatca tcgcgttttt cataacgacg
    10501 agcgggggat tttctttttc tcaaaatgtc gcggaggcca aaaaatcatc cgtcaccaaa
    10561 tggcagctaa agcttcaaaa aagcggtgtg gatacggcgc tttggcagaa atatcttccc
    10621 gatatcaatc gcaaggatta tatcaagata acgaagcttg cggcgagctc accggcgacg
    10681 aataccggaa cggaaacaac agcaaccgct cagccggcgg cgacaacgtc cggatcgctc
    10741 ggaccggaaa tctcggtggg actgcggtac aattcaaaat ccgatacctt caagatcgat
    10801 gccaacaaga cgtataacat tagagacggc ggtgaaaatg gaaaagtcat cgcccagatt
    10861 gcgggcggtt ccacgaccaa agtggactat gacggcaacg gcaatttgaa agtatccggc
    10921 tctgttgctg atacgtctgt ttcggatcaa gtttggtttg acgctgccga cggggacaac
    10981 tcgacaatca ttttcaacac ccacggcagc gattcgtatg accattacag aggaaaaatc
    11041 gaagtcaact attatcacgg cgacgacata tatggcggca actcgaacac ggttacgcaa
    11101 atctggctca tcaacaaatt gccgctcgag cagtatgttt ggggaatggg ggaaacgacc
    11161 ggaaccggag acactgacca tgtgcgggtg atgacgacca tcttccggac ttacggggat
    11221 tggtatatcg agaatgccac aaaatacaag cctttgggct tcaaaattcg gagcgactcc
    11281 aattcgcaaa tatatggcgg ctatgactgg gaaaaggatc atccgcatat caaggaagcg
    11341 gccaacgata cccgcgggaa aatcgtgaaa tacaaaggcg acgtggcgct cacaccgtac
    11401 tgttcttata ctgacggaaa aacaagaagt atggatggct acccgtatct caaatcagtg
    11461 aaggacaaca aggcgggaat caaaaagggt ctcaagcctg gcgacggggg caaccatatg
    11521 tggggacttt ccgcgcatgg cgcattgggt tttgctcagg acggaaaatc gtgggattgg
    11581 attttgaagt attattatac taaagtcgat atcagcagct tatgaatccc gttagacaga
    11641 tcatgtttat gggatttatt atatggggac aaaagtaaaa ttttagtaaa tgtctaacgg
    11701 gatgaaaaaa tcgaaaataa ttgcaattat aattttcatc attgcagctg cggccgtttt
    11761 gtattttgcc tatggggtgg tgaagaaacg ctatatgtct ccggcaaaaa atgctggaac
    11821 aagcagcagc gcaacgcaaa accaagccgg aagccaaact gacaatcaaa atgtcgatca
    11881 atccggggac aaccaggtga gcactccctc ggaagttgac aataccgctc cgacaaacgg
    11941 ccagcccgat gttcaaagcg cggattgcga caacaattgc gcgcaatata aagataatgc
    12001 ggacaatttg aaatactgcc aggaggtttg cgggatcact ccggttaaac caaaaaattc
    12061 tgaagaagac tgcgcgaatc tctccggcct agataaagac agctgctggc gggatctcgc
    12121 cgtttcaaaa aaagattttg ccatttgcga caaaatcagc gacgcgaaac tccaaaaagt
    12181 ctgccacaac cgcgtgacgg aagaagtgct taactagtag cttcctgcca tttgcagcgc
    12241 tttttggaag tttattttcc cgccggtgac ggttttgccg gcaaggctcg gttcggggtc
    12301 gactgagttt tttattatcc caattatttg agttgaagat aagttcggtt ttttggaaag
    12361 aatcagtccg gcttcggcgc tgacataggc ggtggagagg gaactgcctt cttcttcgac
    12421 atagcggttt ttcttgagat cgagaccgaa gattgggttg tcgtccgttc ctcccggagc
    12481 ggcgacatcg acggaagtgg cgccatagtc ggaatattca gccagcgcgc cgtttttatc
    12541 tgaagcacct acgcagataa tatttgccaa atcaaaatcg caagggtaca tttttttgtt
    12601 tggatctgtc ccgtcgtggt tgtaaccgat atatgttttc gaatctccgt ttcccgccgc
    12661 cgtgacaaac actccgggat agctggcgat ggtgtctttg agcaccaagt ccaatccttc
    12721 ttctccgtac gcgccccaac tggcgttgat gatggggata ttgcgcgcct tggcatagct
    12781 cacggcggaa atggcttggt cagtcgtgaa gtcgaatttg agcggcatga ttttgaggtt
    12841 gccgaagaag ctcgcacccg cgatgccttt tttgttgttc gttttggcgg caatgatgcc
    12901 ggctacaaat gtcccgtggc cgtcactgtc cgtcggtttc ttttttggat tgtttatgtt
    12961 tgctcctttt gtccttccgc tagtgatgct gtccttgatc tccggcagac tgaggctcac
    13021 tcccgtatca atgacggcga tcgaaacttt gttttttgtt gccgtttcaa gatcccaagc
    13081 ggacggggcg gaaactccgc cggccgtgct cgttgtgtca gccagcggcc actctttgga
    13141 aaaatattga tcgttgggaa cgcgggagag cagtttgtat ttgaagtttg gctggacgga
    13201 aagaatcgcc ggatcttttt cgagtgtttg gataattttc tacgtagaga cgcgattaat
    13261 cgcgtctcta tctcccttcc c
//
LOCUS       DATKGS010000058         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_76179, whole genome shotgun sequence.
ACCESSION   DATKGS010000058 DATKGS010000000
VERSION     DATKGS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76179"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VMQ48_01430"
     CDS             <1..273
                     /locus_tag="VMQ48_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08734.1"
                     /translation="DYVIGKYYLGANHLTLYNIDWPQYNPDYWAAIGPSLKRTENIND
                     LRNDPNGLIIFNGQPDKKYRDDKTFDPTKFVMVAQYKNIALYRFAN"
     gene            751..>2616
                     /locus_tag="VMQ48_01435"
     CDS             751..>2616
                     /locus_tag="VMQ48_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUD08735.1"
                     /translation="MRKIKRNRNRKILIFVIVFLFALSPFRLDKLHFEKTNAEWPEPG
                     SLIVENDDSWSGNKEISHDVVIRNNATLTIEKGTRVEFHKKDNGMAPAIYVENGNIDA
                     RGNEKEKIIFTSSGAPDEGFSIYFESVSNRESFLRYVDIEHGGRKSNAVPIGFQNNFL
                     NSAYAQGGEMPAVFYSLGPVHIENSRFTDNFFSDVQVQAYLEGANEQTSRLEIVNSNF
                     EGSSQDYAMISSISCQTLGASCSSKILLKDDWYGNSAGPTYENYLISFGKKVKGNFQI
                     DSFRENDLIADPAIVIPGIMGSYKAGGKWIIDPMLFTYSDLLDSFQMNGYKKDKNLFD
                     FPYDWKRKNEDTAVDLETKINQIKNTTKISKVDLVGHSMGGLVARYLVENIENNSVDQ
                     LITLGTPNDGSPEAYLKWEAGEGFFTWTEKVARHHFYQEATEAGYSDIFEYIRAEIPS
                     VRELLPDYGYLSKNGAIMPYSDFYPRNIFLEKLNDPAAVAKLSQVRTDNIVGNLDDER
                     STISGIKITKSTVSGRWEDGMPENFYDDKTDQGIIRGRGDETVPISSASAIPGIDPIS
                     FNSSHGDLPTIAQKTIVSRLTGRDESSLHYVNDIDIPNIFLINVFSPVDIQVVSPS"
BASE COUNT          821 a          509 c          560 g          726 t
ORIGIN      
        1 gattatgtca tcgggaaata ctatctcggc gccaaccacc tgacgctcta caacatcgat
       61 tggccgcagt acaacccgga ctattgggcg gcgatcggcc cgagtctgaa gcgaaccgaa
      121 aacatcaatg atttgcggaa cgatccgaac gggctcatca tcttcaacgg ccagccggac
      181 aagaaataca gggacgacaa aactttcgac ccgacaaaat tcgtcatggt ggctcaatac
      241 aaaaacatcg ccctctaccg cttcgcgaat tgaataaatc cctcttcgca aagcttgcca
      301 tcattttatt ttttggtata ataaagttgc caatcattct tgggggaggg aatcaaaaat
      361 gaaaagcaat ttattttgtt ggaaaattat ttcgaagcaa cagccatccc gccgtttttc
      421 ggttgggatt tttttgatat attaatagca agggataatt attaaaaata aaaaatatgt
      481 ttttttcaag cccaagtatg gatctaaagg tgataatctt ttttgtcgcg gttgctttct
      541 tgatttcgct tgttgtctat tttcgaacaa aaaaaatatt tgctccggta gttatttttt
      601 caatattaag caatattgtt ttgcatggaa acgtgaatta tcaatttgca agctactaca
      661 atgtattttg gctgtttaat ttagtcagaa atatttggcc tttaataaat ttagttctag
      721 ctataatttt aataattcat ctaataaaaa atgcgaaaaa taaaaagaaa tagaaataga
      781 aagatattga tcttcgtaat tgtttttctg ttcgcgcttt cgccatttcg actggacaag
      841 cttcatttcg aaaaaaccaa tgccgaatgg ccggaacccg gatcgttgat tgtcgaaaat
      901 gacgattcgt ggagcggaaa caaagaaata agtcatgatg tcgtcatccg aaacaacgcc
      961 actctcacca ttgaaaaggg cacgcgcgtt gaatttcaca agaaagacaa tggtatggcg
     1021 ccggccatat atgttgaaaa cgggaacatc gacgctcgcg gaaacgaaaa agaaaaaatc
     1081 atctttactt cgtccggcgc gccggacgaa ggattttcaa tttattttga aagcgtttca
     1141 aacagggaat cttttttgag gtatgttgac attgagcacg gcggaagaaa aagcaatgcc
     1201 gttccaatcg gttttcaaaa taatttcttg aacagtgcat atgctcaagg cggtgaaatg
     1261 ccggcagttt tctattctct tggaccggtg cacatcgaaa attcccgctt cacggacaat
     1321 tttttctcgg atgttcaagt tcaagcctat cttgaaggcg cgaatgaaca aacgagtcgc
     1381 ttggaaatcg tgaattccaa ttttgaggga agcagccagg attatgcgat gatttcgagt
     1441 atttcctgcc agacgctcgg ggcgagttgt tcttctaaaa ttttgctgaa agatgattgg
     1501 tatggaaatt ctgccggacc cacttatgaa aactacttga tttctttcgg gaaaaaagtg
     1561 aagggaaatt ttcaaatcga cagtttcaga gaaaatgatc tcattgccga tccggcgatt
     1621 gtcatcccgg ggattatggg atcatataaa gccggaggaa aatggatcat cgatccgatg
     1681 cttttcacct acagcgatct tcttgattct tttcaaatga atggatacaa aaaagacaaa
     1741 aacctctttg attttcccta tgattggaaa agaaaaaacg aagacacggc ggttgatctt
     1801 gagacaaaaa taaaccagat aaaaaatacc acaaaaatat ccaaagtgga tctggtgggg
     1861 cacagtatgg gcggactggt ggcaagatat ctggttgaaa atatcgaaaa caattctgtt
     1921 gaccagttga taacacttgg aacccctaat gacggatcgc cggaggctta tctgaaatgg
     1981 gaagccggcg aaggattttt cacttggaca gaaaaggttg ccagacatca cttttatcaa
     2041 gaagcgacag aggcgggata cagcgatatt tttgagtaca tccgggcgga aataccatca
     2101 gttagagagc tacttcctga ttatggatat ttgtctaaaa acggagcaat aatgccatat
     2161 tccgacttct atcccaggaa cattttcttg gaaaaactca atgatccagc ggctgttgcg
     2221 aaattatcgc aggtaagaac cgataatatt gttggaaatc tggatgatga acgaagcact
     2281 attagtggga taaaaattac aaaaagcacc gtttcaggcc gttgggagga tggaatgccg
     2341 gaaaatttct atgatgataa aactgaccaa ggaataatac gaggtcgtgg agatgaaacc
     2401 gtccccatct ccagcgcttc ggcgattcca gggattgacc cgatcagctt caattcttcg
     2461 catggcgatc ttcccacgat agcgcaaaaa acaatcgtaa gtcgcttaac aggaagagat
     2521 gaatcaagcc tgcattatgt gaatgatatt gatattccca atatttttct cataaacgtt
     2581 ttttctcccg tcgatattca agtcgtttct ccttcc
//
LOCUS       DATKGS010000059         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_117584, whole genome shotgun sequence.
ACCESSION   DATKGS010000059 DATKGS010000000
VERSION     DATKGS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117584"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..1248)
                     /locus_tag="VMQ48_01440"
     CDS             complement(415..1248)
                     /locus_tag="VMQ48_01440"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HUD08736.1"
                     /translation="MDIGLLCEIGKVSRGGYYKWLAHSEEQEKDCEDYLLVKKIFDKG
                     KGKFGFRTIQMRLKERKIVMNHKKIIRIMKKYNLATRIRRRNPYKDIMKKSLEHRTFE
                     NKLNRQFQQNVPFKVFCTDITYLPFNHRFAYLSVVKDVASGEVVASNLSGCLGMDLVI
                     DTVENLKQNKNISKFENILIHSDQGFHYTNPLYIEMVKQLKMTQSMSRKGNCIDNAPI
                     ESFFGHLKDDVDYKDCKTFEELSFLIAEYVKYYNHERQQWNLKKMTPVSYRNHLLEFA
                     T"
     gene            complement(1293..1700)
                     /locus_tag="VMQ48_01445"
     CDS             complement(1293..1700)
                     /locus_tag="VMQ48_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08737.1"
                     /translation="MSKRIFTKEQIGKLLQNPCVAKCSAKSITYNPDFKVSAVRQHKE
                     QCATVKEIFVRAGFDLEMIGRETPRWCLKRWSRIYKAKGAEKLSEERRGRLGRPRTKK
                     LTDADRIKRLKAENAYLKAENDFLAKLRAKRAE"
     gene            complement(1860..>2447)
                     /locus_tag="VMQ48_01450"
     CDS             complement(1860..>2447)
                     /locus_tag="VMQ48_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08738.1"
                     /translation="EGGASVTMLETKMNQKTKSSVGAFYNSESGVEWALNKISISSGT
                     ISSTLSANPDGSVSCPISPDCKVYFLKSDGTVITQANFSTLTFDDIKAVRSVGNQGGD
                     TQRAIEAAVAAGGSNCFTYYCNNSRNNACTDSGGAQRYCPSGFQQKYDMGTWGYCVCD
                     GGTALSSWTFSPPGGNCPCQEPNQRPQGKGLVCCN"
BASE COUNT          621 a          532 c          519 g          775 t
ORIGIN      
        1 gggaggatca aaaagagatt gactggctgg aagagcggga ggggaaactt ttcgcgtatg
       61 aattcaaatt ttccaaaaac aaattttctt tcccgaaaga ttttttgaaa gcctatcccg
      121 aatcaaaaac aaaaatcgtc accaaggatg attatctcga gtttctgaag gtcaaatgat
      181 gtcccaggat ggaattttga aagtccttga aggcatcacg acgatggaag aagtggagcg
      241 ggtgacggaa gaatgaccgt agagacgagg caatgcctcg tctctacttt ttggagatta
      301 taataaaaat acatagagat ttgccatggc aaaagtggcc actggctaaa aagaaaaagc
      361 cccgtcccta tgatttgaag tgtaccctct aaagtagaca cgtaataaaa agccctacgt
      421 ggcgaattcc aaaagatgat ttctgtaact cacaggggtc atctttttta aattccactg
      481 ctgtctttcg tggttgtagt atttcacata ttcagcgatt agaaaactca attcttcaaa
      541 tgttttacaa tccttgtaat caacatcgtc tttcaagtgc ccgaagaatg attcaatcgg
      601 cgcgttatca atgcaatttc ctttcctgga cattgattgt gtcattttta gttgcttgac
      661 catttcaatg taaagcggat tagtgtaatg aaatccttga tcggagtgga tcaggatatt
      721 ttcaaattta gaaatgtttt tgttttgttt caaattttcc acggtatcaa ttaccagatc
      781 cattcctaga caccccgata gatttgaggc cactacctcg ccgctggcaa catctttgac
      841 aactgaaaga taggcgaatc tatgattgaa cggcagatag gtaatgtccg tgcagaacac
      901 tttaaatgga acattttgct gaaattgtct gtttaatttg ttttcaaatg ttctgtgctc
      961 caatgatttt ttcataatat ctttgtaggg atttcttcgt ctgattctgg tcgctagatt
     1021 gtattttttc atgatcctga ttatcttttt gtgattcatg acaattttcc tttccttaag
     1081 cctcatttga attgttctga agccgaattt acctttaccc ttgtcgaata tctttttgac
     1141 gagcagataa tcctcgcaat ctttttcctg ctcttctgaa tgcgcgagcc acttgtaata
     1201 cccgcctctt gaaaccttgc cgatctcgca tagcagccca atatccaact tcaattgttt
     1261 gatgatttga tatttctgat taggcttgag ttttactccg ctcgctttgc ccggagcttg
     1321 gctaaaaaat cgttttcggc tttgagatag gcattctctg ctttcaggcg cttaattctg
     1381 tcagcatcag tcaacttttt tgttcttggt cttcccaatc ttcctcttcg ttcttcggaa
     1441 agtttctcag cgccctttgc tttgtagatt ctactccagc gcttcaaaca ccaccttgga
     1501 gtttctcgcc caatcatttc aagatcaaag ccggctctta cgaatatctc tttcacggtt
     1561 gcgcattgct ctttgtgttg cctgactgcg ctgaccttga aatccgggtt gtaggttatg
     1621 gattttgcgc tgcatttggc aacgcatgga ttttgcaaca gtttcccgat ctgctccttg
     1681 gtaaagattc ttttgctcat agctttttgt taattgatta cattatacca aattaaaaag
     1741 tggacaggcg cttttttaag cgtgtccact ttttggggta cagatcagat ttaggttcgg
     1801 ggtttttaaa atatcaacga gaaattataa taccttagcg aagcctggat gcattaaaat
     1861 taattgcaac aaacaagccc tttgccctga ggacgctgat tgggttcctg acatggacaa
     1921 tttccgccgg gtggcgaaaa agtccaggag ctgagggcgg tgccgccatc gcaaacgcag
     1981 tatccccaag ttcccatgtc atatttttgt tgaaaaccag aaggacaata tctctgtgcg
     2041 cctccgctat cagtgcaagc attgttgcgg gaattattac aatagtaggt aaaacaattg
     2101 cttccccccg ccgccaccgc cgcttcaatc gcccgctggg tgtcgccgcc ctggtttccg
     2161 acggagcgga cggctttgat gtcatcgaaa gtgagggtgg aaaaattagc ttgagtgatc
     2221 acggtgccat cgctctttag aaaataaact ttgcaatccg gagaaatagg gcagctgacg
     2281 cttccgtccg gattggcgga caaggtgctt gagattgttc cggaacttat ggatatcttg
     2341 ttgagcgccc attccactcc ggattcggag ttatagaaag cgccgaccga gcttttggtc
     2401 ttttggttca tctttgtttc cagcatcgtg acgctggccc ccccctc
//
LOCUS       DATKGS010000060         4117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_163473, whole genome shotgun sequence.
ACCESSION   DATKGS010000060 DATKGS010000000
VERSION     DATKGS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163473"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1512
                     /locus_tag="VMQ48_01455"
     CDS             163..1512
                     /locus_tag="VMQ48_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUD08739.1"
                     /translation="MPITGVSIMNASEFCGYWWKFFTDPDFQEQQPWMQPIMRFFGLL
                     PPNVWTSMRVPGGLVVWWLMCNGFYISALAAFLLFVLTDWADGKSARYRKQVTPNGGI
                     FDGVADKCLVIPIMGHLLWIFLLQCYSYWGVIIPALFLLMVISEVSRILMPCSRRFDK
                     KKHFYAIWYGKCKFGVQVVLTVVLWFAIFVFPFWPWWPIWISFFLVVALTLSVFSIIF
                     RLYPELERYAADVVTFGNFSCGALAILFALAIEFKTSVALILIAGGLDMADGFIARKT
                     KPPKEKPGLTFGGLADDTGDFVSFALAPAFILFLLGEYIAAVVYLLATSVRLYFFTDQ
                     ERKGKSISGVFRGFPSPAAAIFLGSFLLWEHPISPETIAIIGMTLAVLEVAFFVRWYH
                     FRMIPKVPSLEKVAAGILGIFVFFFIGSGEALSVLSILYLILFFQPVANRRWGWNKN"
     gene            1622..1861
                     /locus_tag="VMQ48_01460"
     CDS             1622..1861
                     /locus_tag="VMQ48_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08740.1"
                     /translation="MTATATESASVSAEVLAACPVAFLAVDSAIGSVIALGRLERELF
                     DRCSALGTSQIEFGAVEKLPLRTILIVHFSFRLIC"
     gene            complement(1996..2817)
                     /gene="lgt"
                     /locus_tag="VMQ48_01465"
     CDS             complement(1996..2817)
                     /gene="lgt"
                     /locus_tag="VMQ48_01465"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HUD08741.1"
                     /translation="MLQIWQHLPEKINPISFSLGPIQVSWYGIMYLVAFAIVYYLVRR
                     RIRTEKLNISFETVQDFFPWAIVGLLLGARLGDVFIYDWGYFQNHLSQIFLPFDIFNH
                     WRYIGIYGMSYFGGLVGIILAFYIFCKIRKINLLEFSDLFIPAVPLAYAFGRLGNFLN
                     GELYGRVTTKPWGMYFPLDITGQLRHPSQLYELFLEGILLFLILWSIKNSPFIKGGSR
                     GILTAFYLIGYGLARFLAEFFRQPDNNWFFLSLTIGQWFSVGIIIVSIAIIQKKH"
     gene            3082..3348
                     /locus_tag="VMQ48_01470"
     CDS             3082..3348
                     /locus_tag="VMQ48_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HUD08742.1"
                     /translation="MDIDKEIITALKKAHSHLGNVIKMVEEKEYCIAILQQNLAVMGL
                     LKSANNKLLERHLNSCFVNVMKGTNEKRKQEMIDEILAINRLSK"
     gene            3353..4063
                     /locus_tag="VMQ48_01475"
     CDS             3353..4063
                     /locus_tag="VMQ48_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HUD08743.1"
                     /translation="MKTKKIFIQGMHCPSCEKLLEGEFKNIPGVRNVKVNRKTDSADI
                     FFEDKEPDFEKIKNIAEKFSYSVSEERPDMGMAPKNSKVFWQDWLKAGLIIIVILFVF
                     RFFQASGLANGISASNKSISYGVAFLVGLVASVSSCLAVVGAVVIAFSEKYSSEKSAS
                     SADAARFFESAVRPNLFFHIGRLGTFFVLGGLLGMVGGELNISGNFVSIYTIVIAVVM
                     AWLFWSVWSRLSRLVSLW"
BASE COUNT         1050 a          867 c         1016 g         1184 t
ORIGIN      
        1 tagaatcaat tcagtttcac tatttattat actaactttt cctaaaaaat attgccaatc
       61 ttgtttatcc gaacggctgc ggcggggttt ttatttgacc aaaaatgaat aagcggagta
      121 caattggagc cagctcttga acaaccaaaa aataacggct ttatgccgat cacgggggtt
      181 tccatcatga acgcgagcga attttgcggg tattggtgga aatttttcac cgacccggat
      241 ttccaggaac aacagccttg gatgcagcct ataatgcggt ttttcggtct gctgccaccc
      301 aacgtgtgga cgtcgatgag ggtaccgggc ggattggtgg tctggtggct tatgtgcaat
      361 ggattctata tttcagccct agccgccttt cttctgtttg ttttgactga ttgggcggac
      421 ggaaaatcgg ctcgttacag gaaacaagtg actcctaatg gggggatctt tgacggagtc
      481 gctgacaaat gtctggtgat tcccataatg ggacacctcc tctggatatt cctcctccag
      541 tgctactctt attggggagt gataattccg gctcttttcc tgcttatggt aatttcggaa
      601 gttagccgaa ttctgatgcc ttgcagtcga aggttcgaca aaaaaaagca cttttatgcc
      661 atttggtatg ggaagtgcaa atttggcgtc caggtggttc tgactgttgt gctttggttc
      721 gctatcttcg tgtttccatt ctggccatgg tggccgattt ggatttcctt ctttttggtc
      781 gtggccctca cgctgtcggt cttctcgatc atcttccggc tctatccgga gctcgagaga
      841 tacgcggccg atgtggttac ttttggaaac ttcagttgcg gcgcgcttgc catcctcttt
      901 gctctagcaa tagaatttaa aacttctgtg gctttaatcc tcatcgccgg gggcttggat
      961 atggcggatg ggttcattgc cagaaagaca aaaccgccca aagaaaaacc cggtctgaca
     1021 ttcgggggtc tcgccgatga cacaggcgat ttcgtttctt ttgccctggc tccggcgttc
     1081 attctctttt tgcttggtga gtacattgcc gcggtggtat atcttttggc aacttctgtg
     1141 cgcttgtatt ttttcaccga tcaggagagg aagggaaaat caatttctgg agtgtttcgg
     1201 ggatttcctt ctcccgcggc tgcgattttc ctgggcagtt tcctgctttg ggagcatccg
     1261 atatcgcccg agacaattgc gatcatcgga atgacgttgg ccgtgctcga agtggctttc
     1321 tttgtccgat ggtatcactt ccggatgatt ccaaaagttc cttcactgga aaaagttgcg
     1381 gcgggaattc tcggcatttt cgttttcttt tttatcggga gcggagaagc cctgtccgtc
     1441 ctttcaattt tatacctgat cctttttttc caacccgttg ccaacaggcg ctggggctgg
     1501 aacaagaatt aagtcaacac aaaccccgcc aacgcgaatg ccgcggcggg ttttttcttt
     1561 ccacaaaaaa acaactcccg gattgggaga ttgttttttg gttcgattag taccggcccc
     1621 tttgaccgcc accgccaccg aatccgcctc tgtttccgcc gaagtccttg cggcgtgccc
     1681 ggttgcattc ctggcagtag attctgccat aggttccgtc atcgcgcttg gtcggctcga
     1741 aagggagctc tttgatcgct gttccgcact cggtacaagt cagattgagt tcggtgcagt
     1801 cgaaaagctg ccgttgagga cgattttgat agtccatttt tcgtttcgcc taatttgtta
     1861 ataatcccgg gactttgtgt ccggaatagc tctcacttag gcgacgactt tatgtatacc
     1921 ttatatatga atatatttgg atactacgcc taactcgatt actgactata atattacacc
     1981 aaagcgggga aaaagtcaat gtttcttttg tatgattgct atactaacaa tgattatccc
     2041 caccgaaaac cactgcccaa ttgtgagcga aaggaaaaac cagttgttgt ccggttggcg
     2101 gaagaattcg gcgaggaagc gggcgaggcc gtagccgatg agataaaagg cggttaaaat
     2161 cccccttgat ccccctttaa taaagggaga gttttttata ctccataaaa tcaagaataa
     2221 taatattccc tctaaaaaca attcatagag ttgggacggg tgccggagct ggccggtgat
     2281 gtcgagcgga aaatacattc cccatggttt tgtcgtcacg cggccatata attcgccgtt
     2341 caaaaaattt cccaaacggc cgaaagcgta ggcgagggga acagcgggaa taaagaggtc
     2401 ggaaaattcg agcagattta tttttctaat tttacaaaaa atataaaaag ccaaaatgat
     2461 cccgacgagt ccgccgaaat aactcatacc gtatattccg atatacctcc agtgattgaa
     2521 aatatcaaag ggcaaaaaaa tttgcgaaag atggttttgg aagtagcccc agtcatagat
     2581 gaaaacatcg cccagtcttg ctcccaacaa taatccgacg atcgcccatg ggaaaaaatc
     2641 ctggacggtt tcgaaagaaa tattcaattt ttccgttcgg atgcggcgtc gcaccaaata
     2701 atacacaatc gcaaaagcga cgaggtacat gatcccgtac caactgactt gaataggtcc
     2761 gagcgaaaag gatatcggat ttattttttc aggcaaatgt tgccagattt gtagcatata
     2821 tttagtatag agtatttttt ggataaaaaa agggcgggtg tgcattgcga cacccgccgt
     2881 gattattcag gtaattattt tttgcttgcg gaggattcgg agcagatttt cggttttttt
     2941 gtgtttattt cttgcttgaa taatagcaaa ttttgaaaga acttgacagg gaattggata
     3001 gtaagataga gtgtacctag gggggaggtg tttcaaggtg aaaagtaaaa gtgaaaaagt
     3061 aaaaaataat cgaaaaagat tatggatatt gacaaagaaa tcatcacagc gctcaagaag
     3121 gcgcacagcc atctcggaaa cgtgatcaag atggttgagg agaaagaata ttgcattgcc
     3181 atcctgcagc agaatttggc ggtgatgggg cttttgaaat cggcgaacaa caaactgctc
     3241 gagcggcatc tcaacagttg ttttgtgaat gtcatgaagg gaaccaacga aaagagaaaa
     3301 caggaaatga tcgatgaaat tttggcgatt aatcggctat cgaaataatt caatgaaaac
     3361 aaaaaaaata tttatccaag ggatgcactg tccgagctgc gaaaaactgc ttgagggaga
     3421 attcaaaaat attcccggcg ttcgaaatgt aaaagtcaat cgaaaaacgg attccgcgga
     3481 tattttcttt gaggacaagg aaccagattt cgaaaagata aaaaatatcg ctgaaaaatt
     3541 tagctattct gtttcagaag agcggcctga tatgggcatg gccccaaaaa atagcaaagt
     3601 tttttggcaa gactggttga aggcggggct gatcattatc gtcatcttat ttgtattccg
     3661 ttttttccaa gcttcgggtc ttgcgaatgg aataagcgcg agcaacaagt cgatatcata
     3721 cggcgtggct tttcttgtcg gattggtcgc gtctgtttct tcatgtctcg ccgtggtggg
     3781 agcggtcgtc attgcctttt cggaaaaata ttctagcgag aaatctgcct catcggcgga
     3841 cgctgcgcgg ttttttgaaa gtgcggtccg gccgaacttg tttttccata tcggacgcct
     3901 cgggacattt ttcgttttgg gcgggctgct tgggatggtc ggcggagaat tgaacatctc
     3961 cggaaatttt gtttctatct acaccattgt tatcgcggtc gtgatggcgt ggcttttctg
     4021 gtcggtctgg tcgcgtctgt ctcgtcttgt ctcgctgtgg taggggcggt cgtcattgct
     4081 ttttcggaaa aatattcgag cgacaaatcc gcctcgt
//
LOCUS       DATKGS010000061         6679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_17391, whole genome shotgun sequence.
ACCESSION   DATKGS010000061 DATKGS010000000
VERSION     DATKGS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17391"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1356
                     /locus_tag="VMQ48_01480"
     CDS             133..1356
                     /locus_tag="VMQ48_01480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HUD08744.1"
                     /translation="MFDISPEKYVRSGIPLVRVQNIKNGMIDDSNMVYLSEEEHSMEN
                     KTELGDFDFVLSKIGTVGEVALLDPKHKKWNFSQNVIGIKLDKVKISPSYILFFLLSK
                     FGQLQIKRAEMGQVQAKLELEDIRNLKIIRIEGEKIFNDYLVKILKSRSGSELFYKQA
                     ENLLLEELGLKNLDVEENLSAIVNFSDILEARRMDAEYFQPKYENLLEVIKKKVKVAK
                     LKDIANVKRGSLINPKFYSESGTPYLRGKDFSSGNLEKESLVYINDKFIPGNETRVEV
                     DDIIFASIGSVGTLALINNEFKDSFISNNTAKISLIEKNYIVPDYFCMVLHSIVGKLQ
                     FEKESTQTAQPKISDSQVKNFMVPILPIETQQKIADLVRQSHEARKKAKELLEEAKRK
                     VEEMIEKGGEKNGKI"
     gene            1343..1546
                     /locus_tag="VMQ48_01485"
     CDS             1343..1546
                     /locus_tag="VMQ48_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08745.1"
                     /translation="MAKYNRNSGKPIMKKDIQTVKILAKKNTPTRIIGLEIGRTEDSI
                     RKIASENSISLKPTNQSPYNRKK"
     gene            complement(1647..1946)
                     /locus_tag="VMQ48_01490"
     CDS             complement(1647..1946)
                     /locus_tag="VMQ48_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08746.1"
                     /translation="MPKEKTYRYYVEHHDDHTNEVLARYLAPKYECDERELWTGRKCQ
                     AWEVPDRRFVNFLKSSKKSLGIEFDAFIQEDNYSLRPFPWPIPKRPMPRRKGFAM"
     gene            complement(2337..5963)
                     /gene="rpoC"
                     /locus_tag="VMQ48_01495"
     CDS             complement(2337..5963)
                     /gene="rpoC"
                     /locus_tag="VMQ48_01495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HUD08747.1"
                     /translation="MNQNSATKVSDFDALRLKLASPDDILSWSHGEVTKPETINYRTQ
                     RYERDGLFCEKIFGPAKDWECYCGKYKKIRYKGIVCDKCGVEVTRSIVRRERLGHIKL
                     AVPVSHIWFLRGVPSKIGLALGMSAQELEKVVYFASYIVMEVDEVARKDAMERLDAEY
                     KGKQKKMSAQGGSASGGNGDEKNSKGESEAEDLKGAYQAGRDELKNLRKFQILSEVEY
                     FNLSLKYGHVFKAGIGAEAIRRVLEGTNLEEEITRINKDINDDKISDKKKLLKQLKLF
                     QGMSQAGIRPEWMLPTVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRIINRNNRLKKL
                     IDIGAPEVISRNEKRMLQEAVDALFDNSARRGQTSVAASTGQKRALRSLADMLKGKQG
                     RFRQNLLGKRVDYSGRSVIVVGPKLKLHQCGLPKKMALELFKPFIINKLIEKEYAHNV
                     RSAGKMVEFEAEEAYEILDEIIEHHFVLLNRAPTLHRLSIQAFQPVLIEGKAIQIHPM
                     VCSAFNADFDGDQMAVHVPLTDEARNEAENIMLSSKNLLKPASGEPITIPSQDMVLGC
                     YYMTHFKKGKKGEGKIFASSEDAKLAYDFGYIEIHSEIEVSMGDTRIKTSVGRILFNE
                     IMPEEIGFVNEEMDKGKLRQIVARSLALAGRDRTAIFVDELKNLSFALVTRSGISWGM
                     DDLTTPVEKDGLIKDAEKKVETVNAQYDMGLLTDRERKGQVIEIWSETKGKITEAVRA
                     SLDPEGPVYAMVYSKARGTEAVVVQMTGMKGLVAGPTGETIELPIKSSFKEGFNVLEY
                     FIATHGARKGMADTALRTATAGYLTRRLVDVAQDAVVRESDCGDQRGSFMYRADSDVV
                     GISFASRITGRFLIEDVIDPKTKKVILKAGELLAKEKADEIEKKKVDKIKIRSVVTCR
                     SRRGICQKCYGLDLGRSDIVQIGQAVGIVAAQAIGEPGTQLTMRTFHIGGVAGTDITQ
                     GLPRVDEIFEARPPKGEAAIAEVDGQVIEIKETEKDKTIRIESTPDKKGKKEVREYVV
                     SAVMNLEVQKDDLVAKGQVLSEGHLDLKKLFKIQGEEAVYRYITKEVQEIYSAQGEGI
                     NDKHIEVIIRQMLSRVRILDPGDTTLLPGDIVEDVQLEEANEAAKKNGKNLATGEKLL
                     LGISKVALSTESFLSAASFQETAKVLINAAVVGKEDKLRGLKENVIIGKLIPAGTGYR
                     NSLK"
     gene            complement(5987..>6679)
                     /locus_tag="VMQ48_01500"
     CDS             complement(5987..>6679)
                     /locus_tag="VMQ48_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HUD08748.1"
                     /translation="VISKIAALEDMPYLPDGSPVDIILNPLGVASRMNIGQILEAHLG
                     WAASKLGYKAASPALDGVSEKDIKAELKKAGLPEDGKIGLINGKTGEHFDEKTMVGYM
                     YVMKLNHLVEDKIHMRSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGSAYMLQ
                     EMMTIKSDDQLGRSKAYESIIKGERIKSPNVPASFNVLVNELKGLCLNIEVVGERTDS
                     DKADKEEGKKDN"
BASE COUNT         1688 a         1575 c         1496 g         1920 t
ORIGIN      
        1 atggagaaac tgattgaata tttttatcct acagaattgt aaacaacgct cttaattctt
       61 acaattaaga atattttttc caatagaaaa tacaatgatt ttcgtgaatt aagtctattt
      121 atcaaaaaag gtatttttga cataagccct gaaaaatatg tgagatctgg cattccattg
      181 gtcagggtac aaaatataaa aaacggaatg atcgatgact caaatatggt ttatctttct
      241 gaagaagaac actctatgga aaataaaaca gagttaggcg atttcgattt tgttttatcc
      301 aaaatcggga cagttgggga agttgcgctt ttggatccaa aacataagaa atggaatttt
      361 agtcaaaatg tcataggaat aaaattagat aaagtcaaaa tcagcccgtc ttatatctta
      421 tttttccttt taagtaaatt tgggcagctg caaattaaaa gggctgagat gggacaggta
      481 caagcaaaat tagaattaga agatataaga aatctaaaaa taattaggat tgagggggaa
      541 aaaatattta acgactactt ggtaaaaatc ttaaaatcaa gaagtggatc agaattattt
      601 tataaacaag ccgaaaactt gcttttggaa gaattaggtt tgaaaaattt agatgttgaa
      661 gaaaatttat cggccattgt gaatttttcg gatattttgg aagcgcgaag aatggacgcg
      721 gaatattttc agccgaaata tgaaaattta ttagaagtaa taaagaaaaa agtgaaagtc
      781 gcaaaattaa aggatatcgc aaatgttaaa agggggtctc ttatcaatcc gaaattttat
      841 agcgaaagcg gaacgcctta cttgcgcggt aaagattttt catcaggcaa tttggaaaaa
      901 gaaagcctcg tttatataaa tgacaaattc attccaggca atgaaacgag agttgaggtt
      961 gacgatataa tttttgcttc cattggaagc gtcggcaccc ttgcgctaat aaataatgaa
     1021 ttcaaggact ccttcatatc aaataacact gcaaaaattt ctttgataga gaaaaattat
     1081 attgtccctg attatttttg catggttttg cattcaatag ttggaaaatt acaatttgaa
     1141 aaagagtcga cgcaaacagc gcaaccaaaa atatctgata gccaagtgaa aaactttatg
     1201 gttcctattt tgccaattga aacccaacaa aaaatcgccg atcttgttcg ccagtcccac
     1261 gaagcgcgaa agaaagcaaa agaacttttg gaagaagcaa aaagaaaagt cgaagagatg
     1321 atagagaaag gaggtgaaaa aaatggcaaa atataataga aattcaggaa aaccgataat
     1381 gaaaaaggat attcagacag tcaaaatact tgctaagaaa aatactccca caagaattat
     1441 tggtttggaa attggaagaa cggaagattc gattcgaaaa attgcttcgg agaatagcat
     1501 tagtttaaag ccgacaaacc agagtcctta taataggaag aaataactaa aatacaatat
     1561 aatatgtaga taatagccag tctcccaaag gactggtttt tgtttcagaa ataattcgag
     1621 gcacccgttg gggcgcctct tttttactac attgcgaagc cttttcttct tggcattggc
     1681 cttttgggga tgggccaggg aaagggacgc agagaataat tgtcctcctg gatgaaagcg
     1741 tcgaattcga ttccgaggga tttcttgctg cttttcagaa aattaacgaa ccgacgatcc
     1801 ggcacttccc acgcctgaca cttcctgccg gtccagagtt ctcgctcgtc acattcatat
     1861 ttcggagcga gataccgcgc cagcacctcg ttagtatgat catcatggtg ttcaacatag
     1921 tagcgatatg ttttttcttt gggcatagcc accctccttt tcgacgttct attttccaca
     1981 gatgattgta ctttctattc tggtttttgt ccagtatatc tcaatatgac aaatttcaaa
     2041 ataacgaaaa taatccttta tacaaggcat tatttttgaa atgtttcacg ccaaaaagaa
     2101 aaatccccgt gtgatgtgta ccctctaaag tagacatgga ttgacaaagc ttaaaaatct
     2161 gctatactga acttaggcgt tattattcat aatgcctata aaaacaagcg tcgcccgttg
     2221 tgggccatgg cttgtttttg tttcgcctaa gattcaattt ttcggttatc ttcaaggttc
     2281 gaccttttcc tttcccgact attcgtcggg aaattcctca aggtcaaacc ttattattat
     2341 ttcaaactgt ttcgataccc tgtccccgcc gggatcagtt ttccgatgat gacattttct
     2401 ttcagtccgc ggagtttgtc ttcctttcca accaccgccg cgttgatgag aactttggca
     2461 gtttcctgga aggacgccgc cgagaggaaa ctttcggtcg aaagagcgac tttcgaaatt
     2521 ccgaggagga gcttttctcc ggtcgcgaga tttttgccat tcttcttcgc agcttcgttg
     2581 gcttcttcga gctggacatc ttccacaatg tcgcccggca gaagagtcgt gtctcccgga
     2641 tcaaggatcc gcacgcgcga gagcatttgg cgaatgatca cttcgatatg cttgtcgttg
     2701 atgccttcgc cctgggcact atatatttcc tgcacttcct tggtgatata tcgatagacg
     2761 gcttcttcgc cctggatttt gaaaagtttc ttgagatcga ggtggccttc ggaaaggacc
     2821 tgccccttgg cgacgaggtc gtctttttgg acttccaggt tcatcacggc cgagacgaca
     2881 tattcgcgga cttctttctt tccctttttg tccggagtgg attcgatacg gatcgttttg
     2941 tccttttccg tttctttgat ttcgatcacc tgtccgtcca cttccgcgat ggccgcttct
     3001 cctttcggag gacgagcttc gaaaatttca tcaacgcgcg gaagaccctg ggtgatgtcc
     3061 gttccggcga cacctccgat gtggaaagtc cgcatcgtca gctgggttcc cggttctcca
     3121 atggcttgcg ccgcgacaat tccgaccgcc tggccgattt ggacgatatc actccggccg
     3181 agatccaagc catagcattt ctggcagatt ccccggcggc tgcggcaagt gacaaccgaa
     3241 cggatcttga ttttgtccac ttttttcttt tcaatctcat ccgccttttc cttcgcgagg
     3301 agctcgcccg ccttgagaat gacttttttc gttttgggat cgatcacgtc ttcgatcaaa
     3361 aatcttccgg tgatccttga agcgaaactg atgccgacca cgtccgaatc ggctcgatac
     3421 atgaaactgc cccgctgatc gccgcaatcg gattcgcgca caaccgcatc ctgcgccaca
     3481 tcgacgagac gccgcgtgag atatccggcc gtcgccgtgc gaagagccgt atcagccata
     3541 ccttttcgag ctccgtgagt ggcaatgaaa tattcgagaa cgttgaatcc ttctttgaat
     3601 gaagatttga tcggcagctc gatcgtttca ccggtcggtc cggccaccag ccctttcatt
     3661 ccggtcatct ggacgaccac cgcttccgtt ccacgagcct tggagtatac catcgcatag
     3721 accggacctt ccgggtcgag agatgctcgc accgcttcgg tgatcttgcc tttcgtttca
     3781 ctccaaatct cgatcacctg tccttttctt tcccgatcag tgagaagtcc catgtcatat
     3841 tgcgcgttga ccgtttccac tttcttttcc gcgtccttga tcaagccgtc cttttccacc
     3901 ggtgtggtca aatcatccat accccaactg atccccgaac gggtgacgag agcaaaggaa
     3961 aggtttttga gttcatccac aaagatggcg gtccggtctc ttccggcgag cgccaatgag
     4021 cgcgcgacaa tttggcgcag ctttcctttg tccatttctt cattcacaaa tccgatttct
     4081 tccggcatga tttcattgaa caaaattctt ccgactgacg ttttgatcct cgtgtcaccc
     4141 atactcactt cgatttctga atgaatctcg atatatccaa aatcataggc gagcttcgcg
     4201 tcttcgctgc tggcaaaaat cttgccttcg cccttttttc cctttttgaa atgggtcata
     4261 tagtagcagc ccagcaccat atcttgcgat ggaattgtga tcggttcgcc gctggccggc
     4321 ttcaaaaggt tctttgacga aagcatgata ttttccgctt cgttgcgcgc ttcatccgtg
     4381 agcggcacgt gaacggccat ttgatctccg tcgaagtcag cgttgaaagc ggagcaaacc
     4441 atcggatgga tctggatggc ttttccttca atgagaaccg gctggaaagc ctggatgctc
     4501 aagcggtgca aggttggggc gcggttcaaa aggacgaagt gatgctcgat gatctcgtcg
     4561 aggatctcat aggcttcttc cgcttcaaac tcaaccattt ttcctgccga tcgcacgttg
     4621 tgggcatatt ctttttcgat cagcttgttg atgatgaaag gtttgaaaag ttcaagcgcc
     4681 atttttttcg gcagaccgca ttggtgcaat ttcagtttcg gtccgacaac gatcacgctg
     4741 cggccggaat agtccacccg ctttccaaga aggttctgac ggaaacggcc ttgtttccct
     4801 ttgagcatat ctgcgagtga acgcaaagcc cgtttttgtc ctgtcgaagc ggcaacggat
     4861 gtttgtccgc gccgggcaga gttgtcgaag agcgcgtcta ctgcttcttg gagcattctt
     4921 ttttcgttcc gcgagatcac ttccggggcg ccaatatcga tcagcttttt gaggcgattg
     4981 ttgcggttga tgattcggcg gtagagatca ttgagatccg aagtcgcaaa ccggcctccg
     5041 tccaattgga ccatcggacg aagatccggc gggatcaccg gaatgacagt tggcagcatc
     5101 cattccggac ggattccggc ttggctcatt ccttgaaaaa gcttgagttg cttcaaaagt
     5161 tttttcttgt ctgaaatttt gtcgtcattg atatctttgt tgatccgtgt gatttcctct
     5221 tccagattgg tgccctcgag gacgcggcgg atggcttcgg caccgattcc cgctttgaaa
     5281 acgtgcccat atttgagaga caggttgaaa tattccactt cggaaagaat ttggaatttt
     5341 cggaggttct tgagctcatc ccgtccggct tgataggcgc ctttcagatc ttcggcctcg
     5401 ctttcgccct tagagttctt ttcgtctccg tttccgccag aggcggatcc gccttgggcg
     5461 gacattttct tctgtttgcc tttgtattcg gcgtccagtc tttccatagc atctttgcgc
     5521 gccacctcgt ccacttccat cacgatgtag ctcgcaaaat ataccacttt ttcgagctcc
     5581 tgcgctgaca taccaagagc caggccaatc ttcgaaggca cgcctcgcaa aaaccagata
     5641 tgggaaaccg gcacggccag tttgatgtga ccaagacgtt cgcggcgcac aatggagcgc
     5701 gtcacttcca ctccacactt gtcgcagaca atccccttgt agcgaatttt tttgtatttt
     5761 ccgcaatagc actcccaatc ctttgctggt ccgaatattt tttcgcaaaa aaggccgtcg
     5821 cgctcatagc gctgggtgcg ataattgatc gtttccggct tcgtcacttc accgtgagac
     5881 caactcaaaa tatcatccgg gctggcgagc ttcaggcgga gcgcgtcgaa gtcgctaact
     5941 ttggttgcgg aattttgatt catatattat atatagatga aaaaatctaa ttatcttttt
     6001 ttccttcttc cttgtccgct ttgtcagaat ccgttcgttc gccaaccact tcaatgttca
     6061 aacacaagcc tttcaattcg ttgacgagaa cattgaacga cgccggaacg ttcgggctct
     6121 tgattcgctc gcctttgatg atcgattcat aggctttcga gcggccgagt tgatcatccg
     6181 atttgatggt catcatttct tggagcatat aggcgctgcc gtatccttcc agcgcccaca
     6241 cttccatttc tccgaagcgc tggccgccga attgggcttt cccgccgaga ggctgctggg
     6301 tgatgagaga gtatggcccg atggagcgca tatgaatttt gtcttccacg aggtggttca
     6361 gcttcatcac atacatatac ccgaccatcg ttttttcatc gaaatgctcg ccagttttgc
     6421 catttataag gcctattttt ccatcctccg gaagacccgc tttcttgagt tcggctttga
     6481 tgtccttttc actcactccg tcgagcgcgg gactggccgc tttgtagcca agcttcgaag
     6541 ccgcccagcc gagatgcgcc tccaggattt gtccgatgtt catgcgcgat gccactccca
     6601 gcgggttcaa gatgatgtcc acgggagagc cgtccgggag atagggcata tcctcaagcg
     6661 ctgcaatttt ggagatcac
//
LOCUS       DATKGS010000062         7410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_92658, whole genome shotgun sequence.
ACCESSION   DATKGS010000062 DATKGS010000000
VERSION     DATKGS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92658"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..644
                     /gene="rpsI"
                     /locus_tag="VMQ48_01505"
     CDS             162..644
                     /gene="rpsI"
                     /locus_tag="VMQ48_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HUD08749.1"
                     /translation="MATAKKIPKKESKVVEPAAKTVSAEKYFQGIGRRKLSTARVKVF
                     VFAGKKVSKAEPEIVVNGKPYREYFTLLELKEIVASPLKAVSPDGISKVSVAVRGGGI
                     RGQAESVRLGIARALVLKDQEFKKTLKDLGYLTRDARMVERKKPGLKKARRAPQFSKR
                     "
     gene            738..1385
                     /locus_tag="VMQ48_01510"
     CDS             738..1385
                     /locus_tag="VMQ48_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08750.1"
                     /translation="MEKEPVTSEEENKEESSENGREKMNEWFTENLRMIVSVVIVVVI
                     AGGIYSYSKRAQAPNMKSAATSTEQNSEGKISVIGGDNQAASDNQQAAPEATAQPQTQ
                     PSENVQSAQPQTSQETGTSFVETAAKGEGSTNLARRALANYLEKNPDSSLTAEHKIYI
                     EDLLRRQVKDGRMKIGDKREFSKDMIAKAIEKSKSLNDKQLKNLQKYSHRVSALK"
     gene            1534..3006
                     /locus_tag="VMQ48_01515"
     CDS             1534..3006
                     /locus_tag="VMQ48_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUD08751.1"
                     /translation="MAIFYRKLFFWIFVLLFLTTTPVAILYSQGYRFDQYKKIFIHSG
                     SITVKSTPASASIYLNGKLQSSSAFNIINNSTTINGLRPGNYDLKISADGYGTWEKNV
                     DVHSGVSTEFWNIVLAPQKNTPKELSADGALRYFPSPFGKNVAYVKKNGENMEIWSIV
                     RSSNVPTLILSQKDFDFSSDAFENIEWNTKETLFIVPVDHNGKNDFVVLDSGKGQDPI
                     FLSQITGLSGMDHVRWSPANQNEIYFVAKDDSGAQDNLYRMDLGAGKPEIILEGIKAY
                     DLSKNSIYFLQQNNIVYKTDLDGKNEDQLVLAPIDLSDANEKTRLIAYDDNRQAIVSE
                     SGELYVHNNGETTGDTLKKIADGIKSVQFSNDGKKLLYWSDNEINVLYLRKWDVQPRR
                     EENEVQQIVRISSPVKNVFWYRDYEHIFFSTQNSVKIIELDSRDHRAADDIFKYNSEN
                     FLSSYDGANGIYYYVDEIDGTRKLFYLYIPAQTNFFGGGG"
     gene            complement(3337..4209)
                     /gene="prmC"
                     /locus_tag="VMQ48_01520"
     CDS             complement(3337..4209)
                     /gene="prmC"
                     /locus_tag="VMQ48_01520"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HUD08752.1"
                     /translation="MTIREILIKTGSTYNLSPLDMGILLSLALVKPKEYILAHPEKKI
                     SSVQIKKYHSFAKRRSDGEPIAYIAGKKEFFGLDFIVDKNVLIPRPETELLVENAIEK
                     IKNAKCDMPNTIIDVGTGSGNIIISIAKNIPSKIKNKTNFYAVDISKKSLEVAKENAK
                     KNKIDQKIKFIQSNLLEYFQPLVEKNKKFKNILIIANLPYVSPKIYNANKNNLKFEPK
                     TALLSRNNGLDHYIKLFKQVRLLFTVHCSLILEFSPEQKSEISRIITRYLPKAKAEFF
                     KDLAGRWRVAVLSF"
     gene            complement(4343..5404)
                     /gene="prfA"
                     /locus_tag="VMQ48_01525"
     CDS             complement(4343..5404)
                     /gene="prfA"
                     /locus_tag="VMQ48_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HUD08753.1"
                     /translation="MQNQLDAIKTEYQKLKTEMENPEVVSDSQKMKTVGKRMAELEET
                     IAKITELEKMEKDMRENAEIVNSDTEAELKEMAMEENVKLSKQKEELEEKLKKMIVPK
                     DPHDSRNVIVEIRAGAGGDESALFAAELLKMYLRYAEKKGWKTGVMSSSPNSIGGFKE
                     VIASIEGKNVFGKMKYESGVHRVQRVPETEKMGRVHTSTATVAVLPEAEEVDLDINPK
                     DLRIDTFCSSGPGGQSVNTTYSAVRITHIPTGTVVSCQDQKSQIKNKEKAMKVLRSRI
                     LAEQEEKRMKELGAERKSQIGTGDRSEKIRTYNFPQDRITDHRIKESWSNIGGILDGD
                     IDKILGTLEEEDIKRRIST"
     gene            complement(5624..5827)
                     /gene="rpmE"
                     /locus_tag="VMQ48_01530"
                     /pseudo
     CDS             complement(5624..5827)
                     /gene="rpmE"
                     /locus_tag="VMQ48_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            6007..6813
                     /gene="rpsB"
                     /locus_tag="VMQ48_01535"
     CDS             6007..6813
                     /gene="rpsB"
                     /locus_tag="VMQ48_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HUD08754.1"
                     /translation="MQNDEKTPVVGEKKEGEKSAAFSYENLELNIAEMLKAGVHFGHK
                     SSRWNPKMKEYVFGVKNNIHIIDLEKTIGLLQKALEFLRSTVENGGKILFIGTKPQAR
                     QLVEEVSNILDMPYIKNRWLGGTFTNYSEIKRRIKYLNDQEHLLESDELKKYTKYEQS
                     RIRKEIARMNEKMGGIKKMDKLPQAVFVIDVKENALAVKEAQSAGIPTVALTDTNTDP
                     TLVDYPIPANDDALSALKYILGIVAKNIMEAKKRIKIEKTEKAETKQNVK"
     gene            6821..7282
                     /gene="tsf"
                     /locus_tag="VMQ48_01540"
     CDS             6821..7282
                     /gene="tsf"
                     /locus_tag="VMQ48_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HUD08755.1"
                     /translation="MTDIKISAELVKELREKTGASIMECRNALVEAKGDLEKAVENLR
                     KRGKEKAAKKADRATAEGVVVSYIHSNKKIGAMVELLCETDFVAKNSEFQELAYDLAM
                     HITAMEPSDEISLLSQPFVKNPDTTVEDLIKEKIAKIGENIQIGNFVRFEI"
BASE COUNT         2237 a         1526 c         1622 g         2025 t
ORIGIN      
        1 ttgtttggga atccgtctat ggcatgcttc caaaaaataa gctgcgtgac ctgatgatga
       61 aaaagatgct catcttcaag gatgacaagc acggcgtgca caatgtcgaa atcgaagaaa
      121 taaaggcgca aaaagcttaa gcttcaaata tcatatccat tatggcaaca gcaaaaaaaa
      181 ttccgaaaaa agaatcaaaa gtcgttgaac ctgcagccaa aacagtttcg gcggaaaaat
      241 atttccaggg aatcggacgg agaaaacttt ccacggcccg ggtgaaagtt tttgttttcg
      301 ccgggaaaaa agtatcaaaa gccgagcccg aaatcgtggt caacggaaaa ccgtataggg
      361 aatatttcac attgctcgag cttaaagaaa ttgtcgcctc tcctttgaaa gcggtttctc
      421 ctgatggaat ttcgaaagtt tcagtcgcgg tgcggggcgg cgggatccga ggccaggctg
      481 aatcggtgag attgggcatt gcgagagcat tggttttaaa agatcaagaa ttcaaaaaga
      541 cgctcaagga tctgggctat ctgacccgtg acgcaagaat ggttgaaagg aagaagcccg
      601 gactgaaaaa agcccgaaga gccccgcaat tctcgaagcg ctaacaattt ttctgaaaat
      661 tgacaagatt ttagcaaaat tgctatcata aaactcaaag aacattaata acttagctag
      721 aaaggaaggt gtataaagtg gaaaaagaac ccgtcacttc tgaagaggaa aataaagaag
      781 aatcttcaga aaatgggagg gaaaaaatga atgagtggtt cacggaaaat cttcggatga
      841 tcgtgagcgt cgtgatcgta gttgtcatcg ccggggggat atactcatat tccaagcgag
      901 ctcaggctcc gaacatgaag agtgctgcaa catcaacgga acagaattca gaaggaaaaa
      961 ttagcgtgat cggcggagat aaccaagcag caagcgacaa tcagcaggcc gctccggaag
     1021 caaccgcgca gccgcaaaca cagccgtcag agaatgttca gtccgcgcag ccgcaaacca
     1081 gtcaagaaac gggaacttct tttgtggaaa ctgccgcaaa gggagaagga tcaacaaatc
     1141 tggcgcgaag agctcttgcc aactatctgg aaaaaaatcc ggattcatcg cttactgccg
     1201 aacacaagat atatattgag gatcttctgc gccggcaagt caaggatggc aggatgaaaa
     1261 ttggcgacaa gcgtgaattc agcaaggata tgattgcgaa agcaatcgaa aaatcaaaga
     1321 gtctcaatga caagcaactc aagaatcttc aaaaatattc acatcgagtt tcagcattga
     1381 aataaccatt tgaaaaatat cgttctttct gatataaaac caaatgaccg gcaaccgccg
     1441 gttttttggt acgaaaatga gtacaaaaat tagacaaagt caagaagata ctttagtata
     1501 aagttggtaa atttgagata gtttcagaat attatggcta ttttttatcg caaattgttt
     1561 ttttggatat tcgtccttct ttttctgaca accaccccgg tggctatttt gtattcccag
     1621 ggttatcgtt tcgaccagta caagaaaata ttcatccata gcgggtcgat cactgtgaag
     1681 tcaaccccgg ccagtgccag catatatctc aacgggaagc ttcaatcatc cagcgcgttc
     1741 aatataataa acaattcgac gacaataaac ggcttgcgtc ccgggaacta tgatctgaaa
     1801 atttcggcag acggatacgg aacttgggaa aaaaatgttg atgtgcacag cggagtgtca
     1861 actgaatttt ggaatatagt ccttgctcct caaaaaaata ctccaaaaga actttccgcc
     1921 gacggcgcac tgcgatattt tccatcacct tttggaaaaa atgtggccta tgtcaaaaaa
     1981 aatggagaaa atatggaaat atggtctatc gtcaggagca gcaacgttcc gacgctcatt
     2041 ctttcccaga aagactttga tttttcaagc gatgcctttg aaaacataga gtggaatacg
     2101 aaagagacat tattcattgt tcctgtcgat cacaacggca agaatgattt cgtcgttttg
     2161 gattcaggga aagggcagga tccaatattt ctctcccaga tcaccggtct ctccggaatg
     2221 gatcatgtcc gttggagccc ggccaatcaa aatgaaatat attttgtcgc gaaagacgac
     2281 agcggcgctc aagataactt gtataggatg gatcttggcg cagggaagcc ggaaatcatt
     2341 cttgagggaa tcaaggcgta tgatctttcg aaaaattcaa tatattttct tcagcaaaac
     2401 aatatagttt acaaaaccga tctggatggg aagaatgaag accagctggt cttggcgccc
     2461 atagatcttt ctgatgccaa tgagaaaaca aggctcatcg cctatgacga caaccggcag
     2521 gccattgttt ccgaatccgg cgaactgtat gtccacaaca acggcgaaac aacgggagat
     2581 actctcaaga aaatagccga tgggataaaa agcgtgcagt tctcaaatga cggaaagaaa
     2641 ctcctttatt ggagtgacaa tgaaatcaac gtactatatc tgcgaaagtg ggatgttcag
     2701 ccgaggagag aggaaaatga agtccaacaa atcgtccgta tctcatctcc cgtgaaaaat
     2761 gttttttggt atcgcgacta cgagcatatt ttcttcagta cgcaaaattc cgtgaagatc
     2821 atcgagctcg attcgcgcga ccatagggca gccgatgata ttttcaaata taattcggaa
     2881 aactttttga gttcgtatga tggcgcaaat ggaatatatt attatgttga tgaaatcgat
     2941 ggaacaagaa aactatttta tctttatatc cccgcacaaa caaatttctt cggaggagga
     3001 ggctaataaa aaatattttt tcttaaattg aaataatttt tcacttgcga gagtaataat
     3061 actcgaaagt gcggcctgtg gataactttt ttgacttgca attgaaaaat aatccatcta
     3121 taataaaaac aattccaaaa acattccgag aaataaaaaa taaaattgaa gggaggtgaa
     3181 acacaatggc aaagaaaaaa aaagcagcca agaagaaagt tgcaaagaaa aaagtagcaa
     3241 agaaaaaaaa gaaaagatag tctgaatcaa ttttcttttt gcaatagaaa acaaaggcgc
     3301 cccaatcgga gcgtctttgt gttttttctg tttgtctcaa aatgacagta cggccacccg
     3361 ccatcttccg gccaaatctt tgaagaattc agccttggct ttcggcagat atctggtgat
     3421 aatacggctt atttcagatt tttgctcggg actaaattca aggatcagtg agcagtgaac
     3481 agtgaacaac agcctgactt gcttgaataa ttttatatag tgatccaatc cgttgtttcg
     3541 actaagcagg gctgttttcg gttcgaattt caaattgttt ttattggcat tataaatttt
     3601 cggcgagacg tagggaagat tagcaatgat aagaatattt ttaaattttt tatttttctc
     3661 aaccaaaggc tgaaaatatt ccagaagatt tgattgaata aattttattt tttgatcaat
     3721 tttatttttt ttggcattct cttttgcgac ttctaaggat tttttcgaga tatcaactgc
     3781 ataaaagttt gttttatttt taattttact tggaatattt tttgcgattg aaataataat
     3841 atttcctgat ccggtgccga cgtcgatgat agtatttggc atatcgcatt tggcattttt
     3901 tattttttca atagcgttct caactagcaa ttcagtttcc ggtcgaggaa tcaaaacatt
     3961 tttatccaca atgaaatcca atccgaaaaa ttcttttttc ccggcgatgt aggcaatcgg
     4021 ctcgccgtcg gatcttcttt tggcaaatga atgatatttt ttgatttgca cggacgaaat
     4081 tttcttttcc ggatgtgcca gaatatattc tttcggcttc accagcgcca aagacaaaag
     4141 aatccccatg tcgagcgggg aaagattata agttgagcct gtttttatta atatttctcg
     4201 tattgtcata taaacaagag aaaattttca atttcagaat tttcaatttt caaacaatga
     4261 ctgaattatt caatttcaaa atgtttctga aattcggtca tttgagaatt gtttgaaaat
     4321 tttggaattt tgacattgaa aattacgtcg atattcttcg cttaatatct tcctcctcca
     4381 gcgtgcctag tatcttatca atatccccat caagaattcc tccgatattg ctccaacttt
     4441 ctttgatgcg atgatccgtg attcggtctt gcgggaaatt gtaggtccgg attttttccg
     4501 accggtctcc ggttccgatc tggctttttc gctccgcacc cagttctttc atcctttttt
     4561 cttcttgttc ggccaaaatg cgggagcgca aaactttcat cgctttttcc ttgtttttga
     4621 tctgcgattt ttggtcttgg caagaaacaa cggttccagt ggggatatga gtgatccgca
     4681 cggcgctata ggtcgtgttg acgctctgtc cgcccggtcc tgacgagcag aaagtatcaa
     4741 tgcgcaaatc ctttggattt atatcgagat ccacctcctc ggcttcagga agaacggcca
     4801 ctgtggcggt tgaggtgtgg actcggccca tcttttcggt ctccggcact ctttgcacgc
     4861 gatgtacgcc tgattcgtat ttcattttgc caaaaacatt tttgccttcg atgctcgcga
     4921 tcacttcttt gaagccgccg atcgagttgg ggcttgaact catcacacct gttttccagc
     4981 cctttttttc cgcgtagcgc agatacattt tcaaaagctc ggccgcgaag agggctgatt
     5041 cgtcccctcc ggctccggcg cggatttcga caatgacgtt tcggctgtcg tgcggatctt
     5101 tcgggacaat catttttttg agtttttctt caagttcttc tttctgcttt gacaatttaa
     5161 cattttcttc catggccatt tctttgagtt ccgcttccgt atcactgttc acaatttccg
     5221 cgttttcccg catatctttt tccatttttt caagctctgt gattttcgcg attgtttcct
     5281 ccaattccgc cattcttttc ccgacagttt tcattttttg ggaatcagaa accacctcgg
     5341 gattttccat ctcggttttc agtttttgat attcggtttt gatcgcgtcc agctgatttt
     5401 gcatatcact agtcttaata atcgcaaaga aaggaagctt tgtcaaaaaa agaccgcact
     5461 ctgcggcctt tttaaatttg taggtgtcgg acacatacaa gaactatttt gcagtctttg
     5521 ctgttttttt cttggcaacc tttttctttg cgactggttt agctggttta gctgcaggtt
     5581 ttttcttttt ttcagctctt tccactttat ccgccagcat ttgttcgctc ttttccatgc
     5641 gcttcttgaa acgatcaacg cggccggtcg aatcaacgag gcgctttttg ccggtataaa
     5701 aaggatggca ggcgctgcag acttcgactt tcattgcctc tttcacggaa ccggttgtgc
     5761 tcacattccc gcaggcgcag ataattttgg cttcagagaa gtatttcggg tggatatctt
     5821 ttttcataat ttttcgaaaa tcaataaatt tacccattaa tcctagcata acatccaata
     5881 attgacaagc cctcacgctc ttgctacact atatatcgtt atcaaacata tctcccagag
     5941 gcaaatcctt gtcctcgcct agcggggtaa tccgggggat agaagtaaat aattaaagga
     6001 aaaacaatgc aaaacgatga aaagaccccc gtggtcgggg aaaaaaagga gggcgagaaa
     6061 tcggccgctt tttcgtatga aaacctggag ctgaatatcg ccgaaatgct caaggctggc
     6121 gtccatttcg ggcacaaaag ttcgcgctgg aacccgaaaa tgaaggaata tgttttcggc
     6181 gtgaaaaaca atattcatat cattgatctt gaaaaaacga tcggattgct gcaaaaggcg
     6241 ttggaatttt tgcgctcaac cgtggaaaac ggcggaaaaa tacttttcat cggtacaaag
     6301 ccccaggctc gccagctggt tgaagaagtc tcgaatattc tcgatatgcc atacatcaag
     6361 aatcgctggc tgggaggaac cttcacaaac tacagtgaga tcaagcgccg gatcaaatat
     6421 ctcaacgatc aggaacacct tctggaaagc gatgagctca agaaatacac caaatacgaa
     6481 caaagccgga tccggaaaga aattgcccgg atgaatgaga agatgggagg aatcaagaaa
     6541 atggacaaac tcccgcaggc ggtctttgtg atagacgtca aggagaacgc cctggcagtg
     6601 aaggaagcgc agagcgcggg gattccgaca gttgccctca cggacacgaa taccgacccg
     6661 acgctggtcg actatccgat tccggccaac gacgacgccc tttccgccct gaaatatatc
     6721 ttgggaattg tggcgaagaa tatcatggag gcaaagaaaa ggataaaaat agaaaagacg
     6781 gaaaaagcgg aaactaagca aaatgttaaa taacaaaaaa atgaccgaca tcaaaatatc
     6841 cgccgagctc gtgaaagagc tgcgcgaaaa aaccggagcg agcatcatgg aatgccggaa
     6901 tgcgcttgtt gaagcgaaag gcgatcttga aaaggccgtg gaaaatcttc ggaagcgtgg
     6961 caaagagaaa gctgccaaaa aggccgaccg ggcgaccgcg gaaggcgtgg tcgtttccta
     7021 tattcacagc aacaagaaaa taggagcgat ggtcgaactg ctttgcgaga cggactttgt
     7081 tgcaaaaaat tccgagttcc aggaactggc ctatgacctt gcgatgcata tcacggccat
     7141 ggaaccatcc gacgaaattt cgcttttgag tcagccgttc gtcaagaatc cggatacaac
     7201 agtcgaagat ctgatcaaag aaaagatcgc caaaattggt gaaaatatcc agatagggaa
     7261 ttttgtgaga tttgagattt aatcaatgct gcaaactgtt tttctcaaaa taaggcgctg
     7321 ggaaagttcc cgtccggtca aatgcgccca aacgctcgaa acgggtgata tcgttattgt
     7381 ggaaacggaa gacggccttg attcggcaat
//
LOCUS       DATKGS010000063         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_163726, whole genome shotgun sequence.
ACCESSION   DATKGS010000063 DATKGS010000000
VERSION     DATKGS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163726"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1535)
                     /gene="murC"
                     /locus_tag="VMQ48_01545"
     CDS             complement(159..1535)
                     /gene="murC"
                     /locus_tag="VMQ48_01545"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HUD08756.1"
                     /translation="MIDFSKIKKIYFIGIKGSGMVALVEYFDRKGFKVTGSDTHEKFF
                     TDEILKKKLFVKYHEGFDAKHITNDIDLVIYSTAYNEKNNEEWKAAKYKMIPMISYPE
                     MVANLFNEKYGIAVCGTHGKTTTTAMLAEIFKAAGLDPTAVVGSQVRQWRGSALVGRS
                     EFMIIEADEYQNKIGLYKPQSVILTSADWDHPDFFPTFEEYKDTFAKFVEKIPRHGFL
                     VVCGDRSETLEIAKSAKCKVISYGFGKDVDLKIVNHEIREGKQFFSINMRDKKLADFE
                     IMLPGRHNILNATAAIAMAHQFNAKIDKVREALANFEGTSRRFEYIGQRNGAILIDDY
                     GHHPEEIKATLQAAREFFPKRRIWCVFHPHTFTRTKALLSEFAQSFEDADKVIVLDIY
                     GSAREVQGGVHSKDLVELAKRYHRGVEYISTIPEAVEFLKDKISREDLVIAMGAGNVW
                     EVVEGLKE"
     gene            complement(1546..2496)
                     /locus_tag="VMQ48_01550"
     CDS             complement(1546..2496)
                     /locus_tag="VMQ48_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUD08757.1"
                     /translation="MSRKKIGVALGGGILRGMAHIGALEVLAENDIVPDILTGCSSGA
                     LVAAAYGCGTLGELKKISLSVDRKSRRQMLDFCLTGEGLIRGKKMRSFFDFITGGKNF
                     EDIEGIKLAFVGTDALTGKEVVIDEGNIAEALEITTALPGLAPLKRHKGRLVFDGGTA
                     MLVPAKIAYDLGAEYVIAIDVGAERSLMTRLLGDVRGLMRKTRVGKMAAPMFRMQKMI
                     INSDEHNFLGKARELMQRLQLLDDYVNHEYNFLETYLIGLKTITSDYERGIFHKEQAD
                     IAIRPEVFHINRSDVTKIDEIIQAGRKGVEKKLEKIKKIF"
     gene            complement(2496..3608)
                     /gene="murG"
                     /locus_tag="VMQ48_01555"
     CDS             complement(2496..3608)
                     /gene="murG"
                     /locus_tag="VMQ48_01555"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HUD08758.1"
                     /translation="MRIVLTGGGTGGHIFPIIAVVKKVRELAPEGADLEFLFLGPDGD
                     LEREAMEKEMIPAKKILSGKMRRYFSASYIPDIFKVPIGIVQSLWQLLVFMPDVVFSK
                     GGYAGVPVVVAAWLYRIPIVIHESDIMPGLANQFAGKLAKKVMVSFPGAANFFSPEKV
                     EIAGNPIREELMKGNKEEAKKTFGLPEGRKTILVMGGNQGAKVINESILHILPKLVKI
                     FEIIHLTGGSDYESVVQEAGRSGIKAGHGSGYHPYPFLREEMAQALAVADLVVSRAGA
                     NAISEIAANGKPAIIIPIKSSANNHQELNAYALSESGAAVVLEQDNLGGNIFFEKIEE
                     VMNSGELQFELRERIKKFYNPDAAEKIALELINLAN"
     gene            complement(3611..>3930)
                     /locus_tag="VMQ48_01560"
     CDS             complement(3611..>3930)
                     /locus_tag="VMQ48_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HUD08759.1"
                     /translation="DSIFAIIAEEIGLIGAAAMIILFVIFAMRGLKIAKSAPDTFGKL
                     VAVGVTSWVVFQALMNIAAISGIVPLTGVTLPFISYGGTSLVFLMIGVGILLNISKKC
                     DKL"
BASE COUNT          968 a         1056 c          797 g         1109 t
ORIGIN      
        1 ctgctaatat ctcctcacta agatcaagag ctctgagata aaaatcaaaa tctcttttgt
       61 atatttctcc atacactgcg aattgagctt tatcaagaac gctatcattt tttctttctg
      121 ctcgtaagtt ttctattgtc ataagggtat ttcttttttt actcttttaa cccctccacg
      181 acttcccaaa cattgcccgc gcccatggcg atgacgaggt cttcgcggct gattttgtct
      241 ttcaaaaatt cgacggcttc gggaatggtg gaaatatact cgacaccgcg gtgatatctt
      301 ttggcgagtt caaccagatc tttcgagtgc acgccgccct gaacttctcg cgcgctgcca
      361 taaatatcaa gaacaatcac cttgtcggcg tcttcaaaac tttgggcgaa ttccgaaagc
      421 aacgcttttg tccgcgtgaa agtatgcgga tgaaaaacac accagatccg gcgtttgggg
      481 aaaaattccc gcgccgcttg aagcgtggcc ttgatttctt cgggatgatg gccgtagtca
      541 tcaatgagaa tcgccccgtt cctttggccg atatattcaa accggcgcga agttccctcg
      601 aaattagcga gagcctcccg gaccttgtca attttggcat tgaattgatg cgccattgca
      661 attgcggctg tggcatttaa aatattatgc cggccgggta gcattatttc aaaatccgca
      721 agctttttgt cacgcatatt aatcgaaaaa aactgcttgc cttcacggat ttcatgattc
      781 acaattttca aatccacatc tttcccaaac ccataggaaa tcactttgca tttcgcgctc
      841 ttggcaatct ccaaagtttc gctccggtca ccgcaaacga ccaaaaatcc gtgacgggga
      901 attttctcga cgaacttcgc gaaagtatct ttatactctt caaaagtcgg aaaaaaatcg
      961 ggatgatccc agtcggcgct ggtgaggatc accgattgag gcttgtataa gccaattttg
     1021 ttctgatatt catccgcttc aatgatcata aattcgctcc ggccgaccag cgcgctgccg
     1081 cgccactgcc gaacctgcga tccgacaacc gctgttgggt caagtccggc cgctttgaaa
     1141 atttccgcga gcatcgccgt cgtggtcgtt tttccgtgcg tcccgcaaac cgcaatccca
     1201 tatttttcat tgaacagatt ggccaccatt tccggatacg aaatcattgg gatcattttg
     1261 tatttcgccg ccttccattc ttcgttattt ttttcattat aagccgtaga ataaataacc
     1321 aagtcgatat cattcgtgat gtgcttcgcg tcaaagcctt cgtgatattt cacgaacagt
     1381 tttttcttga gaatttcatc cgtgaaaaat ttttcgtgcg tgtcggatcc cgtcaccttg
     1441 aatcctttgc ggtcaaaata ctcgaccagc gccaccatcc cgctgccttt gatgccgatg
     1501 aaatatattt tcttgatttt tgaaaaatca atcatagaaa cgatcctaaa atattttctt
     1561 tattttttcc agcttttttt ccaccccttt tcttcccgct tgaataattt catctatttt
     1621 tgtcacatcc gaccggttga tatggaaaac ttccggccga atggctatat cagcttgctc
     1681 tttgtggaat attcctcttt catagtccga ggtgatggtc ttgaggccga tgaggtatgt
     1741 ttccaaaaaa ttgtattcgt gattcacata atcgtcgagc agctgcaatc tttgcatcaa
     1801 ttctcgcgcc ttgcccaaaa aattgtgctc gtccgagttg ataatcattt tttgcattcg
     1861 aaacatcggc gcagccattt ttcccacgcg agttttgcgc atcaaccctc gcacatctcc
     1921 aagcaaacgc gtcatcaggc tcctttccgc cccaacgtca atggcaatca catattcggc
     1981 tccgagatca taggcaattt tggccggcac cagcatggct gttccgccgt caaaaacaag
     2041 ccgccccttg tgccttttga gcggtgccag tcctggaaga gcagtggtta tctcgagcgc
     2101 ttcggcaata tttccttcgt caatgacaac ttctttcccc gtaagcgcat ccgttccaac
     2161 aaacgccaac ttgatgcctt cgatatcctc aaaatttttt ccgcccgtga tgaaatcaaa
     2221 aaaagaacgc atttttttcc ctctgatcag accttctccg gtgagacaaa aatcgagcat
     2281 ctgccggcgt gattttctgt caacgctcaa ggaaattttt ttgagttcac caagcgtccc
     2341 gcagccatag gctgcggcta caagcgctcc actggagcaa cccgtcaaaa tgtcgggtac
     2401 aatatcattt tccgcgagca cttccagcgc tccgatatgg gccatccctc tcagtatccc
     2461 tccgccaaga gccacgccga tctttttcct gctcatcaat ttgccaagtt aattagctcc
     2521 aaagcgattt tttccgctgc gtcaggattg tagaattttt tgatcctttc tctgagttca
     2581 aattgcagct cgccgctatt catcacttct tcaatttttt cgaagaaaat attcccgcca
     2641 agattatcct gctcgaggac aaccgccgca cctgattctg aaagagcata ggcattgagc
     2701 tcctggtggt tgtttgcgga tgatttgata ggaatgatga ttgccggttt tccgttggcg
     2761 gcgatctcgg aaatggcatt tgctcccgcc cgactgacca caaggtccgc tactgccaaa
     2821 gcctgcgcca tttcttcgcg caaaaatgga tagggatgat agccgctgcc gtgtccggct
     2881 tttatgccgg atcggcccgc ttcctggacg acgctctcat agtcgctgcc gcccgtgaga
     2941 tggataattt caaaaatttt caccaatttc ggcaaaatat ggagaattga ttcattgatg
     3001 actttcgctc cctgatttcc acccatgact aggattgttt ttctgccttc aggaagtccg
     3061 aaagttttct tcgcttcttc tttatttcct ttcataagtt cttcgcgaat ggggttcccg
     3121 gcaatttcaa ctttttcggg actgaaaaag ttggccgctc cgggaaaaga aaccatcact
     3181 ttcttcgcca gctttcccgc gaattgattg gcaagacccg gcatgatgtc cgattcatga
     3241 ataacaatcg ggatgcgata gagccacgct gcaaccacaa ccggcacgcc cgcgtatccg
     3301 cccttggaaa aaacaacgtc gggcatgaag acaagcagct gccaaagcga ctggacgatg
     3361 ccaatgggaa ccttgaagat atcaggaata tagctcgcag aaaaataccg gcgcattttt
     3421 ccgctcaaaa ttttcttggc cggaatcatt tctttttcca tcgcctctct ttccaaatcg
     3481 ccatccggtc ccaaaaacaa aaattccaag tccgcacctt cgggcgccaa ctctctcact
     3541 tttttcacaa cagcgataat cgggaaaatg tggcctcccg ttccgccgcc ggtcaataca
     3601 atacgcatat ctatagttta tcgcatttct tggaaatatt caacaaaatt cccaccccaa
     3661 tcatcaagaa aaccagggac gttcctccat agctgatgaa aggcaaggtg acccccgtga
     3721 gcggaactat gccggaaatg gccgcgatgt tcatcagagc ctggaaaacc acccatgaag
     3781 tgacacccac tgcgaccaat tttccaaacg tatccggcgc gcttttggcg atcttgagcc
     3841 cccgcatcgc aaaaatcaca aaaaggatga tcatcgccgc cgctccgatc aaaccgattt
     3901 cctcggcgat gatcgcaaat atggaatcgc
//
LOCUS       DATKGS010000064         6045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_150089, whole genome shotgun sequence.
ACCESSION   DATKGS010000064 DATKGS010000000
VERSION     DATKGS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150089"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VMQ48_01565"
     CDS             complement(<1..1078)
                     /locus_tag="VMQ48_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HUD08760.1"
                     /translation="MLDEIKKIKFDKLEKLKKAGMDPYPEKTVRAMTNKEALNKFDEL
                     QEKATVLVGRVKSLRPMGGSAFAHIEDESAKMQIFLSKKNIDPEKYKLFVDTIELGDF
                     IEVKGKLFKTKTEEITLEVSDWKILSKSLAPVPTEHFGVKDTEELLRRRYLDLMMNSE
                     TRELFRKKNIFWQTARTFLAKKGFLEAQTSVLENIPGGAEAEPFVTHHNALDRDYFMR
                     ISLELPLKRLLVGGYEKVFEIGRVFRNEGVDREHLQEYDMLEFYEAYTDLKKGMNLVE
                     KLYKEIVEKVCGKMETEYEGNKINWGEKWETKDYFSLFKQETGIDLDQDVSADDLQKK
                     ADKLGIKYEKNYGKGRMIDTIYKKT"
     gene            complement(1284..1739)
                     /gene="greA"
                     /locus_tag="VMQ48_01570"
     CDS             complement(1284..1739)
                     /gene="greA"
                     /locus_tag="VMQ48_01570"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HUD08761.1"
                     /translation="MTKFLTPEGRKKLEEELAMRKTEMRQKIAEIIKEAKEQGDLSEN
                     AEYSEAKRQQSDNERRIMEIENDVRTSQIASFNQESNVVQMGSKVKVKCNGDEQDFCI
                     VGSNESDPLDGKISNESPIGQALMGKSADEKVRVNTPAGEKEYTVVEVK"
     gene            complement(1851..3740)
                     /gene="mrdA"
                     /locus_tag="VMQ48_01575"
     CDS             complement(1851..3740)
                     /gene="mrdA"
                     /locus_tag="VMQ48_01575"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUD08762.1"
                     /translation="MFENYFNKKRQLRGAEIDEITENSSDLDETRVVGSAVSGNRLKV
                     FRVIVFVFFALLVARVFYLQIARGGYYSELSRENRVRSIVIKAPRGIIYDRNGKKLVN
                     NVPSFDLIAIPADILKDQNLKKQEMADIAEMFGMNSQNVEVIVGSQNENSMNPVLIKE
                     NVSQDESLIFAEKYLNLKGFALDQTAVRQYEDGPYFSSFIGYSGKISKDDLVAHPDYL
                     MTDYIGKTGVEYGYEKNLRGVNGKQEVEVDSAGNIQKDFGVTEPVPGSDLVLGIDADL
                     QKKLEDSLQAKLDETGTKTAAAVAIDPRNGEILAMVNIPSYDNNLFAKGISADDYQKL
                     MSDPDKPMFNRSISGEYPPGSTFKPLVAAAALQEGTITPSTTLDCPAAINIGSYSFPD
                     WKTHGLTDVRKAIAESVDIFFYAVGGGWGDITGLGIDKIHEYADKFGLGRKLGIDIPG
                     ESSGLIPTQQWKQDKLGERWYLGDDYHCAIGQGFVTATPLQLANYIAAIANGGTVFQP
                     HFVDSIKKIDGSVEKVNPVVLNKNFISAANLQVVREGMRQTVISGTAQPLKDLPVEAA
                     GKTGTAQFGSGNSREHGWFVSFAPYNNPTIAMAVLVEGGGEGFSTAEPVTHDVYQWYF
                     GDRNK"
     gene            complement(3752..4297)
                     /locus_tag="VMQ48_01580"
     CDS             complement(3752..4297)
                     /locus_tag="VMQ48_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08763.1"
                     /translation="MKNFALLIFCVVTVFFQLSIEGVFFSARRIPDLALALVITLVLT
                     MGFKESLKWILLTGILIDAGSSTVFGTATLAYFLVGWMVAGLIDVADIKSRKTFFLAA
                     FAVAVLVLEIVKDIFIFAIFKIKANFLHEPFGASLNIFSADYFFKILYTIIAAYAIYY
                     IFRRVSRALFLEPVRLVKRNY"
     gene            complement(4294..5094)
                     /gene="mreC"
                     /locus_tag="VMQ48_01585"
     CDS             complement(4294..5094)
                     /gene="mreC"
                     /locus_tag="VMQ48_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HUD08764.1"
                     /translation="MHFFSRKNLKYLSIAGVLIIVIFASFRSSNNPVKGAILTVSSPF
                     LKTSRIFFGGVSGFFQFMGSIGDLKSENEKLVGENQSLLAQNNRLKDAEKENQTLRQQ
                     LNLAPRNNFDLEASFVIAQDPQGLGNYFLIDKGENSGIKTGMAAIVSNGILVGQVTES
                     YSNSAKVTLITDPSSAVNAEVQSSGAKGIVNGEYGLGLKMGMISQAEVLSEGDTVISS
                     GLGGKIPRGLTIGKIGQVGQSPDKLFQTASVVPAIDVSDLRTVFIIKK"
     gene            complement(5108..>6045)
                     /locus_tag="VMQ48_01590"
     CDS             complement(5108..>6045)
                     /locus_tag="VMQ48_01590"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUD08765.1"
                     /translation="AVNQKTGQILAIGAEAKKMVGKTPAHIVATRPLVDGVISDFEVT
                     EQMLRYFIEKVHKDSFALMPRPQVVIGIPSGVTEVEKRAVVDAAMSAGARRAYLVDEP
                     MAAAIGARLPIQEASGNMIVDIGGGTTEIAVISLGGVVVCRSLRIAGDEMNEDIIRYA
                     RDEFNLLLGLKTAEDIKIAIGSAYELDEVLEMPMRGRDLVTGLPKEVIVNDSQIREAI
                     FRSIKIIVNNIKSTVEETPPELLSDIMQRGIILAGGGSLIRGLEKLIAEQTDIPTRVM
                     DDPLTAVARGTGIILEHLDELKNVLAETQYDKVSF"
BASE COUNT         1518 a         1599 c         1210 g         1718 t
ORIGIN      
        1 cggttttttt gtatatcgtg tctatcatcc gtcctttgcc atagttcttt tcatatttga
       61 ttcccagttt atccgctttt ttttgcaaat cgtcagctga aacatcttga tcgagatcaa
      121 ttccggtttc ttgtttgaac agtgaaaaat agtctttcgt ttcccatttc tctccccaat
      181 ttattttatt cccttcgtat tctgtctcca tctttccgca aactttttcc acaatttctt
      241 tgtataattt ttcaacaaga ttcattccct tttttaggtc cgtataagct tcataaaatt
      301 ccagcatgtc atattcctga agatgctcac gatcgacgcc ttcattgcga aaaacccgtc
      361 cgatttcaaa tactttttcg tatccgccaa ccagcagcct cttgagcggc agctcgaggg
      421 aaatacgcat gaaataatcg cggtcaagcg cgttgtgatg agtgacgaag ggctcagctt
      481 cagctccacc gggaatattt tcaagaacgg aagtctgcgc ttccaaaaat ccttttttgg
      541 cgagaaaagt tcgagccgtt tgccaaaata tgtttttctt gcgaaaaagt tcgcgcgttt
      601 cggaattcat catgagatcg agataacgcc ttcgcaaaag ttcttccgta tctttcacgc
      661 caaaatgttc cgtcggcacg ggagccaggc ttttgctcaa tattttccaa tccgacactt
      721 caagagtgat ctcctctgtt ttggttttga acaattttcc tttgacctcg atgaaatcgc
      781 caagctcgat cgtgtcgaca aaaagcttat atttttccgg atcaatattt tttttgctca
      841 aaaaaatctg catcttcgcc gattcatctt caatgtgtgc aaaagccgac ccgcccatcg
      901 gacggagcga tttcacccga ccgaccaaaa ctgtagcttt ttcctgaagt tcatcaaatt
      961 tattcagcgc ttccttgttt gtcatcgccc gtacggtctt ttccggatag ggatccatac
     1021 ccgctttttt aagtttctct aatttatcaa attttatctt ttttatctcg tcgagcataa
     1081 tctaaaattt tcaattatca atttacaatt ctcaatcaat gatcaattct tcaattttca
     1141 atgtttgaaa attagtacat tgaaaattta ttgaaaattg agccttgaaa attgaaaatt
     1201 gtcaacatta atattataca tttttacgct aaaaaaacaa gctctagtca aaactagcgc
     1261 ctgcttaaaa tccaataaaa tatttacttc acttccacca ctgtatattc tttttctccc
     1321 gccggagtgt taactctgac cttttcatcc gcgcttttgc ccatcaaggc ctggccgatc
     1381 ggtgattcat tggaaatctt tccgtccaaa ggatcggatt cgtttgatcc gacaatgcag
     1441 aaatcctgct catctccatt gcacttgacc ttgactttgg atcccatttg gacaacatta
     1501 ctttcctggt tgaagctggc aatttgcgag gtgcgcacat cattctcaat ctccattatc
     1561 cgccgctcat tgtcggactg ctggcgcttc gcttccgagt attccgcgtt ctcgctcaag
     1621 tcgccctgct ctttggcttc cttgatgatc tcagcgatct tctggcgcat ttccgttttg
     1681 cgcatcgcca gctcttcttc aagttttttg cgtccttccg gggtgagaaa ttttgtcatt
     1741 tgttttcttg ataagggata taacgattag aatgatagtt gaaaaagaaa gtgttgtcaa
     1801 taagaaaata atgtttatac cacagcctaa aataggcctt atataagcta ttatttattt
     1861 ctatccccga aataccattg gtacacatcg tgcgtcaccg gttcggctgt cgagaagcct
     1921 tctcccccac cttccaccag cactgccatg gcgatggtcg gattgttgta gggcgcgaaa
     1981 gaaacaaacc aaccgtgttc ccgactgttc ccgcttccaa actgcgccgt cccggttttt
     2041 cccgcggcct cgactggcag gtctttcaaa ggttgggccg tcccggagat caccgtctgc
     2101 cgcatccctt cccgcacaac ctgcaaattg gctgcggaaa taaaattttt attcaaaaca
     2161 accggattca ctttttccac ggatccgtca atttttttga tcgaatcgac aaagtgcggc
     2221 tgaaaaaccg ttccgccgtt cgcaatcgcg gctatatagt tggcaagctg aagaggcgtc
     2281 gccgtcacga aaccttgccc gatggcgcaa tgatagtcat ctcccaagta ccaacgctcg
     2341 ccaagtttgt cctgtttcca ctgctgggtc ggaatgagcc ccgaagattc tcccggaata
     2401 tcaatcccga gtttgcggcc gagaccaaac ttgtcggcgt actcatgaat cttgtcgatt
     2461 ccaagtcctg tgatatcgcc ccagccgccc cccaccgcat aaaagaatat gtccacgctt
     2521 tccgcgatcg cttttcggac atccgtgagg ccgtgcgttt tccagtccgg aaaactatag
     2581 ctcccgatat tgattgctgc cggacagtca agcgtcgtgc tcggcgtgat cgtcccctct
     2641 tgaagcgccg ccgccgcaac aagcggtttg aaagttgaac cgggcggata ctctccggaa
     2701 atcgaacggt tgaacatcgg cttgtccgga tcggacatta atttttgata gtcgtccgct
     2761 gaaattcctt tggcgaaaag attgttatca taacttggaa tattgaccat cgccaaaatt
     2821 tcgccgtttc tcggatcgat cgcgaccgcc gccgctgttt tcgtccccgt ctcgtcaagc
     2881 tttgcctgca gagagtcttc gagctttttc tgaagatcag cgtcaatccc caagaccaaa
     2941 tcgctccccg gcaccggctc cgtgacgccg aaatcctttt ggatattccc ggccgagtcc
     3001 acctcgacct cctgtttccc atttactccc cgcagatttt tttcatatcc atattccacg
     3061 ccggtttttc cgatatagtc cgtcatcaga tagtccggat gagcaacaag atcgtccttt
     3121 gatatttttc cgctatatcc gataaaggaa gaaaaatatg gaccatcttc atactggcgc
     3181 accgccgtct gatcgagagc gaagcccttg agattcaggt atttttcggc aaaaataaga
     3241 gattcatcct ggctcacgtt ttctttgatc agaacgggat tcatcgaatt ttcattttgc
     3301 gatccaacga tcacttcgac gttttgactg ttcatgccaa acatctcggc aatatccgcc
     3361 atctcctgtt ttttcaaatt ttgatctttc agaatatcag ccggaatggc gatcaggtcg
     3421 aaactcggga cgttgttcac aagctttttt ccgttccggt cgtatatgat tccccgcggc
     3481 gccttgatga caattgagcg cacccggttt tcccgcgaca gttcgctata atatccgcct
     3541 ctcgctattt gaagataaaa aacccttgcg acaaggagcg caaaaaacac aaaaacgatc
     3601 acgcgaaaaa ctttgagcct gtttcccgaa acagcacttc caacaacacg cgtttcatca
     3661 agatcggaag aattttccgt gatctcatcg atttcggctc ctcgcaactg tctttttttg
     3721 ttgaaataat tttcaaacat ggaaatattt atcaataatt ccttttcacc aatcttacgg
     3781 gttccaaaaa caacgctcga ctgactcttc tgaaaatata ataaatagca taagcagcaa
     3841 tgattgtata taaaatcttg aaaaaatagt ctgcactgaa aatgttcaaa gaagcaccga
     3901 atggttcgtg gagaaaattt gcttttattt taaaaatggc aaaaataaat atatctttca
     3961 caatctccaa aaccaggaca gcaacggcaa aggctgccag aaaaaatgtt ttccgcgact
     4021 ttatgtcagc cacatcgatg agaccggcaa ccatccatcc gaccagaaaa tacgccaaag
     4081 tcgccgttcc gaaaacggtg cttgatccgg catcgatcaa aattcccgtg agcaatatcc
     4141 atttaagaga ttccttgaaa cccatagtca aaaccagagt gatcacaagc gccaacgcca
     4201 gatccggaat cctccgcgcg gaaaaaaaga cgccttcaat ggagagctgg aaaaaaaccg
     4261 taaccacgca aaagatgagc aatgcgaaat ttttcatttc tttatgataa ataccgttct
     4321 caagtctgac acatcgatgg ccggaacaac cgaggccgtc tgaaagagtt tgtctggcga
     4381 ttgtccgact tggccgatct tgccaatcgt gagtcctcgc ggaattttcc cgccaagacc
     4441 tgacgagatc accgtgtcgc cttcgctcaa aacctccgct tgcgagatca tgcccatttt
     4501 gagtcccaat ccgtattctc cattcacaat tcccttcgcc ccggagcttt gcacctcggc
     4561 gttgacggca ctcgagggat cggtgataag cgtgactttc gccgagttcg aatatgattc
     4621 agtaacctgt ccgaccaaaa tcccgtttga aactatggcc gccattcccg ttttgatccc
     4681 ggaattttct cctttgtcaa tgagaaaata attgccaagc ccctgcggat cttgggcaat
     4741 gacaaaactg gcctcaagat caaaattgtt tctgggagca agattcagct gctgtcgcaa
     4801 agtttgattt tctttctccg catccttgag ccggttgttt tgggcaagca agctttggtt
     4861 ttccccgacc aatttttcgt tttcactttt gagatctccg atcgatccca taaattgaaa
     4921 aaaacccgac actcccccga aaaaaattcg ggaagttttg agaaacggac tggaaacagt
     4981 cagaattgct cccttcaccg gattgttgga agagcgaaaa ctggcaaaga tgacgattat
     5041 caatacgcct gctattgaaa gatattttag gtttttgcgc gagaagaaat gcatagaaaa
     5101 aattatacta gaaactcact ttatcgtatt gcgtctcggc caaaacgttt ttcagctcat
     5161 cgagatgttc caggatgatt cccgttcccc gcgccaccgc cgtcaaggga tcatccatca
     5221 cgcgcgtggg aatatcggtc tgctcggcga tcaatttttc gagcccccgg atcaagctgc
     5281 ccccgcccgc cagaatgatg cctctttgca taatgtcgga aagaagttcc ggcggagttt
     5341 cttcgaccgt cgacttgatg ttgttgacaa ttatttttat ggagcgaaaa atagcttccc
     5401 ggatctgtga atcgttcaca atcacttctt ttggaagtcc cgtgacaaga tcacgtccgc
     5461 gcatcggcat ttccaaaact tcatcgagct cataggcgct tccgatcgcg atcttgatgt
     5521 cttcggccgt cttgagtccc aagagaagat tgaattcatc gcgggcatag cggatgatgt
     5581 cttcgttcat ttcatccccg gcaatacgca agctccggca aacgaccacc ccgccaagag
     5641 agatcaccgc gatttcggtc gtccctccgc cgatatcaac gatcatattc ccggacgctt
     5701 cctggatcgg gagacgcgct ccaatcgcgg ccgccatcgg ctcatcaaca aggtacgccc
     5761 gccgagcgcc cgcgctcatc gccgcgtcca ctaccgcccg cttttccacc tccgtcacgc
     5821 ctgacggaat tccgatgacc acctgcggcc gcggcatcaa agcgaaactg tccttgtgga
     5881 ctttttcaat gaaatagcga agcatctgct ccgtcacttc aaagtccgaa atgactccgt
     5941 ccacgagcgg ccgagtcgca acaatatgag cgggcgtttt cccaaccatt tttttggctt
     6001 ccgcgccgat tgccaaaatc tgaccggtct tctggttcac ggcca
//
LOCUS       DATKGS010000065         4366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_7074, whole genome shotgun sequence.
ACCESSION   DATKGS010000065 DATKGS010000000
VERSION     DATKGS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7074"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VMQ48_01595"
     CDS             complement(<1..743)
                     /locus_tag="VMQ48_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08766.1"
                     /translation="MQISNNSTFIRRNRIYIILGLIIIVGIYLRTYHFHDWLYFYPDQ
                     ARDVLVVKDFLGGKSPLPLMGFRAASTSFDLGAMYYYFQIASGKLFGVAPNTMAYPDL
                     FFNILAIPLLYYFLKRYFKTNISLVLTGLYSISYFAIEYSRFAWNPNPMPFFVLLYLL
                     SLWKFLTEKEKTRWGWIVGIGAAVGIGIQLHTILIFLLTSVSLIALIITLKSDWRLWK
                     RWLLIIGIVLLLNTNQIVSETRHNFSNTK"
     gene            complement(753..2552)
                     /locus_tag="VMQ48_01600"
     CDS             complement(753..2552)
                     /locus_tag="VMQ48_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08767.1"
                     /translation="MKIGDSKIFQYSKKHHIWAVVIAYAFLSVVLTYPLIARFSSEIP
                     QGGRDSFLVMGQIESRSVAMADQGWLAGMKTIFGNRDINSYLPYVILDQFFHNKFATN
                     NFLFLLSFVLSGLGAYLLAFYFTKNRPASFLAGIIFAFSPFHVYQSTAIHLGMRHQEL
                     IPFFVLFLFRFFEKLEIKFFVLIGLFALLIAITEHQLLAFTVLFAVFFVIYKLISDRT
                     LLLNKRLWAFLFASMIFLAIVAVSIFGPLLKVALSSDNYLDPGIKSAINLSIVPADAL
                     LPPVFHGLWPNANEYFQKSIFHTSSKGDSYYAGIVVIGVVLFFLLLFFKEKIRKKETK
                     VNVGMSTALVWFWLVEVIVFFLLSMGPTTAQIGKWTMHFPYYFIYKYIPFYVNIRVVG
                     RLFVFVVLGLSVLFAFAIEYLTKKYPGKKNITVVALGLLLLLDFCIAPMKTDALSYSP
                     FYDRIGKDTQSYKLLEIPGSTDDVFFNYEWITQNVHHKELVNGMPVAREVEGQFDFQK
                     NTPVIEQLLYTIPKGNNPESKGNLKKYQKYYENANEILNENNIVYITISKKYANAEVQ
                     SSAEKFIEKYIKYDSKYEDYFLIAYKVTNVMSK"
     gene            complement(2549..4126)
                     /locus_tag="VMQ48_01605"
     CDS             complement(2549..4126)
                     /locus_tag="VMQ48_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08768.1"
                     /translation="MRQSILEKIKSVPKFWWILLAVLAVGFFLRVYNHHDWLRFNADQ
                     GRDALLVSSVVDGKASLPLLGPKAGGTQFRLGSAFYWFEVAAAKIFGNAPDKMAYPDL
                     FTGILCIPLLFLFLQKYFEKYVSLYLTAIFAVSAYAIFYARFGWNPNSTPFWTLLFLY
                     AIHEVVSKKNKRKYLWSVLAGVAIGVDVQLHTTLLVILPLVTVLVFGYFSFKNKKILK
                     YFLVILIASLIINIPQLIDMHKTNGKNIAAFFQGLKTKQKSESTVAGNILQSTSCLIQ
                     GSIYIASGYEISDKCSFDSGSSKVNIAIFTFGSIFVLGGLILAVRYFLKETNADHKHF
                     LGIVFVFTGITYLVFTKMAYELSVRFYLPLVFLPFILLGFWAEFFREKIKTGHSIILL
                     IAGAFLLGSNLFFVQKSFATFANYGNPGGSDVNITILKEAEEFSRFIVANAGNKKNVY
                     ISGDKEFLHKAYTPLKYLVGRSNIKLSLLKNLPLPDQSFQVVRQKNRKKILPGTDMNI
                     LQYKDYGSFSMLLIQNI"
     gene            complement(4138..>4366)
                     /locus_tag="VMQ48_01610"
     CDS             complement(4138..>4366)
                     /locus_tag="VMQ48_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08769.1"
                     /translation="VAAATQTFREAIEDGVDGFVAGDDGEWKEKLDKLILNGDLRRQM
                     GEIAREKVLENYTNKNSRNKEYYDHLKSKLE"
BASE COUNT         1360 a          946 c          896 g         1164 t
ORIGIN      
        1 ttttttgtat tggaaaaatt gtgccgggtt tcactgacga tttgattggt gttgaggagc
       61 aagacaatgc cgatgatcag cagccaacgt ttccaaagac gccaatcact tttcagcgtt
      121 atgatgagag cgatgagact gaccgacgtc agaagaaaaa ttaaaatagt atgaagttgg
      181 ataccaatgc caacagccgc tccgatccca acgatccaac cccaacgtgt tttctctttt
      241 tcagtgagaa atttccagag agacagcaaa tatagcagca caaaaaaggg catcggattg
      301 ggattccagg caaagcggga atattcaata gcgaaatagg aaatggaata gagcccggtg
      361 agaaccagcg agatattggt cttgaaatat cttttcagga aataatacag cagcggaatc
      421 gccaagatgt tgaaaaacaa atccggatag gccatcgtat ttggcgcgac tccgaacaat
      481 tttccgctgg caatctggaa ataataatac atcgccccca gatcgaaact cgtgctggcc
      541 gcccgaaatc ccatgagcgg caaaggagat tttccgccca aaaaatcttt cacgaccagc
      601 acgtcgcgcg cctggtccgg ataaaaatag agccaatcgt gaaaatgata ggtgcggaga
      661 tatatcccga cgataattat caatccgagt ataatatata tcctgtttcg tcttatgaat
      721 gtcgaattat ttgagatttg catattatcg gattatttgg acattacatt ggtcacttta
      781 taggcaatca gaaaataatc ctcgtattta ctgtcatatt ttatgtattt ttcgatgaat
      841 ttttcggcac tagactgaac ctcagcgttg gcatattttt tgctgatggt tatgtagacg
      901 atgttgtttt cattcaaaat ttcattggca ttttcgtaat atttctgata tttttttaag
      961 tttcctttgc tttccgggtt gtttcccttg ggaatagtat agagaagctg ttctatcacc
     1021 ggcgtatttt tttgaaagtc gaattgcccc tcgacctcgc gcgccaccgg catgccgttg
     1081 accagctctt tatgatgcac attttgagtt atccattcat aattgaaaaa tacatcatcc
     1141 gtgctgcccg gaatttcgag aagcttgtag gattgggtat ctttcccgat tctatcataa
     1201 aaaggagaat acgaaagcgc atctgttttc atgggcgcaa tacaaaaatc aagcagaagc
     1261 aacagcccaa gcgcaacaac tgttatattt tttttgccgg gatatttttt cgtcaaatac
     1321 tcaatcgcaa acgcaaacaa aaccgaaagt cccagaacga caaaaacgaa caatcggccg
     1381 acgacccgga tgttgacata aaaaggaata tatttatata tgaaataata cggaaaatgc
     1441 atcgtccatt ttccgatctg cgcagttgtc ggccccattg acagcaagaa aaaaacgatc
     1501 acctcaacca gccaaaacca aacaagcgcg gtgctcattc cgacatttac cttggtttct
     1561 ttttttctta ttttttcttt gaaaaacagg agcaggaaaa acagcactac tccgatcacg
     1621 acaatgccgg catagtacga atctcccttg ctcgatgtat gaaatatcga tttctggaaa
     1681 tattcgttcg catttggcca caagccgtga aacacaggag gcagcaaagc atcagccggc
     1741 acaatcgaaa gattgatggc gctctttatt cccggatcga gatagttgtc ggatgaaagg
     1801 gcgactttca aaagcggtcc gaaaattgaa accgccacga tcgctaaaaa tatcattgag
     1861 gcgaataaaa acgcccacag ccgtttgttg agaagaagag tccggtcgga aatcagttta
     1921 tatataacaa agaaaacggc gaataaaacc gtgaaagcca gcagctggtg ctcggtgatt
     1981 gcaataagca gcgcaaaaag gcctatcaaa acaaaaaact tgatttccaa tttttcgaaa
     2041 aaacgaaaca ggaaaagcac aaaaaaaggt atcaactcct gatgtctcat ccccagatga
     2101 attgccgttg attggtacac atggaacggg gaaaacgcaa aaataatccc ggcaaggaaa
     2161 gaagccggcc tgtttttggt gaaataaaat gccaaaagat acgcccccaa cccgctcaaa
     2221 acaaaactca acaggaataa aaagttgttt gtggcaaact tgttgtgaaa aaactgatcc
     2281 aggatcacat aaggaagata gctgttgatg tcccgattgc cgaatattgt tttcattcct
     2341 gcgagccaac cctgatccgc catggctacg gatctgctct cgatctggcc cattaccaaa
     2401 aatgagtctc ttcctccttg gggaatttca cttgagaatc tcgctataag cggatatgta
     2461 agcacgacag aaagaaaggc ataggcaatc accacagccc aaatatggtg cttttttgaa
     2521 tattgaaaaa tttttgaatc gcctatcttc atatattttg aataagaagc atggaaaaac
     2581 ttccgtagtc tttgtattga aggatattca tatccgtacc cgggagaatt tttttccgat
     2641 ttttctgtct cacaacctga aaagattgat cgggcaacgg caagttcttg agcaatgaca
     2701 attttatgtt cgatcttcca accagatatt tgagcggcgt ataggctttg tgcagaaatt
     2761 ctttgtcccc ggaaatatat acattctttt tattgcctgc gttggcaacg ataaaccggg
     2821 aaaattcctc ggcctccttg agaattgtga tattgacatc gcttcccccg ggattgccat
     2881 aattcgcgaa cgtggcaaat gatttttgga cgaagaaaag atttgagcca agtagaaacg
     2941 cgcccgcgat caagagaatg atgctgtgcc ctgtcttgat tttttctctg aaaaattcgg
     3001 cccaaaaacc gagcagaatg aagggaagga aaacgagcgg gagatagaat ctcactgaaa
     3061 gctcataggc catctttgtg aaaacgaggt acgttattcc cgtgaaaaca aacacaattc
     3121 ccaaaaagtg tttgtgatcc gcattcgtct cttttaaaaa atatctgaca gccagaatca
     3181 acccgcccaa aacgaatatg gagccaaaag tgaatatcgc gatattcact ttgctgctgc
     3241 cggagtcaaa agaacatttg tcggagattt cgtatcccga agcgatatag atactccctt
     3301 gtatcaagca gcttgtgctt tgaagaatat ttccagccac ggtgctttct gatttttgtt
     3361 tggtcttgag gccctggaaa aaagctgcaa tgtttttccc gtttgtctta tgcatatcaa
     3421 tcaactgcgg aatgttgata atcagcgaag caatcaaaat aaccaaaaaa tatttcaaaa
     3481 ttttcttgtt tttgaaagaa aaatatccga aaacaagaac cgtgaccaac ggaagaatga
     3541 ccaaaagcgt tgtgtggagc tggacatcaa ctccgattgc gacaccggcc aacaccgacc
     3601 acaaatattt gcgcttgttt ttcttggaaa caacttcgtg gatcgcatac aaaaacaaaa
     3661 gggtccagaa aggcgttgaa ttcgggttcc agccaaaacg ggcatagaaa atcgcataag
     3721 ccgaaacagc gaatatcgct gtcagataca aggatacata tttctcaaaa tatttttgaa
     3781 gaaacaaaaa aagcagcgga atacacaaaa ttcccgtgaa cagatccgga taggccattt
     3841 tgtcgggagc gtttccgaaa attttggctg cggctacttc aaaccaatag aaagccgagc
     3901 cgagccgaaa ttgggttccc ccggctttgg gtccaagcaa aggcagggat gcttttccat
     3961 cgacaacgct tgaaacaagc agcgcgtctc ttccttggtc ggcgttgaaa cgcagccagt
     4021 cgtgatggtt gtaaacgcgc aaaaaaaatc caactgccaa aacagccagc aatatccacc
     4081 aaaatttcgg aacacttttt attttttcaa gtatgctttg acgcaaaagt ttgaaaatta
     4141 ttccagctta gacttcaaat gatcataata ttctttgttg cggctgtttt tgttcgtata
     4201 gttttccaaa actttttccc tggctatctc gcccatttgt ctcctcaaat ctccattcag
     4261 aatcaattta tccaattttt ctttccactc tccatcatct ccggccacaa atccgtccac
     4321 gccatcttcg atcgcttctc tgaatgtttg agtggctgca gcgacg
//
LOCUS       DATKGS010000066         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_150231, whole genome shotgun sequence.
ACCESSION   DATKGS010000066 DATKGS010000000
VERSION     DATKGS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150231"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VMQ48_01615"
     CDS             complement(<1..653)
                     /locus_tag="VMQ48_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08770.1"
                     /translation="MKNKNNKKAGFSIMEVVAAIGIFTVVFISVFGSFRAGLASLVQS
                     RHRTEAAALANEKMEIIRNLPYASVGTIGGIPAGSLPQNEAVTKSNQKFNVHTFIRYV
                     DDPQDGSGLADTNHVTTDYKEVKVEVTWGGVSTGHGVVAVSKFVPNGVEADVGGGTFR
                     LNVLDGSGAGFPGVAIHITNSSVNPAVDINTNTDSYGTILMSGMPAGDRNYHITVSK"
     gene            complement(675..>2189)
                     /locus_tag="VMQ48_01620"
     CDS             complement(675..>2189)
                     /locus_tag="VMQ48_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08771.1"
                     /translation="WEGGTIPKDWNVLWYYLQIWKFKNINIFTSDYDFIHEHIPSTKQ
                     LLPIYNYTIPKGSSDPAYYDEMQEYNQYLNDLFWSTNNLFGDNTLIIEGSDVPTTKMI
                     DVKEYQGANQGKIWKDGIPVFDPLHKDETQGDGTVVNASSAFDFLPTDPNHPLFPGAK
                     SEKLSGTSHAQLPTASTAKIHDFLFAQQPPHGDYPLPSENKLVFTFASPVDVKIIASD
                     GKYITKSENTIGKAFYYSDGQSDGPKIVEVEDPASGIYKVELTGNGNGEYHMLASKQN
                     GENNDESEKQGQITVGQILNYKVDTAAENFEIQPEQSADTTPPSIGITSPGGKTYSND
                     QAIPVEYAITDDMSAEGEIVKNIQLDGQVFSGNIIDLSLQKIGGHKLKIIAVDKAGNK
                     SEKEVAFQVTTNLNAIQNNINHYWELKLIKTKCAKRFLTVRLNQLENLFNLLEKTKNS
                     NLKPKPKQVAVDALKKVINADINLLVRQINRKSPRGIDTKVAGLLVEDLNYIRP"
BASE COUNT          526 a          503 c          462 g          698 t
ORIGIN      
        1 tcttttgaaa ctgtgatatg atagttcctg tccccggccg gcatcccgga catcagaatt
       61 gtcccgtagg aatcagtatt cgtgttgata tcgaccgccg gattcacaga gctgttcgtg
      121 atatgaattg ccacccccgg aaaaccggca ccgctaccgt caagaacgtt tagacgaaat
      181 gttcctcctc caacatcagc ttcaacgcca ttgggaacaa actttgaaac ggccacgacg
      241 ccgtgtccgg tcgaaactcc tccccacgtc acttccacct tcacttcttt gtagtccgtg
      301 gtgacatgat tcgtgtctgc cagacccgat ccgtcttgag gatcatccac gtagcggata
      361 aacgtgtgca cattgaactt ctggttgctt ttcgtgacgg cttcgttctg gggcaaactg
      421 ccggccggaa ttccacctat ggttccgacg gaagcatagg gaagattgcg gataatttcc
      481 attttttcat tcgccaacgc cgccgcttcc gtgcgatgcc gcgactgaac caaagaggca
      541 agtcccgctc tgaaagaacc gaacacagat atgaaaacga ctgtgaagat cccgatcgcc
      601 gcgacaactt ccatgatgct aaagccagct tttttattat ttttattttt catttgcctt
      661 tttatacttt ttccctacgg ccggatatag ttcagatctt caacaagcag tcccgccact
      721 ttggtatcga tcccgcgggg cgatttgcgg ttgatctgcc gcacaagcaa attgatatcc
      781 gcattgatga cttttttgag tgcatccacg gcgacctgtt tcggcttggg tttcagattc
      841 gaatttttgg ttttttcgag aagattgaaa agattttcga gctgattcaa tcggacagtc
      901 aaaaaccttt tcgcgcattt tgttttgatc agctttagct cccaatagtg attgatattg
      961 ttttgaatgg cgttgaggtt ggttgtaact tggaaagcaa cttctttttc ggatttattt
     1021 ccagccttat caaccgcaat aattttcaat ttgtgcccgc ctattttttg caatgacaag
     1081 tcgattatat ttccagaaaa aacttgcccg tcgagttgaa tatttttcac aatctcccct
     1141 tccgcagaca tatcatcagt tattgcatat tcaaccggaa tagcctggtc gttagaataa
     1201 gtctttcctc cgggagaagt tattccgatt gaaggaggcg tggtgtctgc gctttgctcg
     1261 ggctgaattt cgaaattttc cgccgccgta tcaactttgt aattcaatat ttgcccgacc
     1321 gttatttgcc cttgcttttc gctttcgtca ttattttcgc cattttgttt tgaggccagc
     1381 atatgatatt caccattgcc atttccggtc aattcaactt tgtatatccc cgaagccgga
     1441 tcttcaactt cgactatttt cggaccgtcg ctttgtccgt cggaataata gaaagctttt
     1501 ccaattgtat tttcgctttt tgtaatgtat tttccgtcag acgcaataat tttcacatcc
     1561 accggcgaag caaaagtgaa aacaagtttg ttttcgctcg gaagaggata atcgccgtgc
     1621 ggaggctgct gggcaaacag gaaatcgtga attttcgcag ttgaggcggt tggaagctgc
     1681 gcgtgactgg tgcccgaaag tttttctgat tttgcaccag gaaacaaggg gtggtttgga
     1741 tctgtaggca aaaaatcaaa agcagaactt gcgttgacaa ctgttccgtc gccttgtgtt
     1801 tcatccttgt gaagcggatc aaaaaccggt ataccgtctt tccatatttt tccttgattt
     1861 gcgccttgat attctttcac atcaatcatt ttggttgtcg gaacatcgct tccttcgatg
     1921 atcagcgtat tgtcgccaaa aagattattt gtgctccaaa aaagatcatt aagatactga
     1981 ttatattctt gcatttcgtc ataatatgca gggtcggatg atccctttgg gattgtgtaa
     2041 ttatatatcg gcaaaagttg tttcgttgag ggaatgtgtt cgtggataaa atcataatca
     2101 ctcgtaaaaa tatttatgtt tttaaatttc catatttgca aatagtacca caggacattc
     2161 caatcttttg gaattgttcc tccttccca
//
LOCUS       DATKGS010000067         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_76899, whole genome shotgun sequence.
ACCESSION   DATKGS010000067 DATKGS010000000
VERSION     DATKGS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76899"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VMQ48_01625"
     CDS             <1..1127
                     /locus_tag="VMQ48_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08772.1"
                     /translation="LSFATLPVITVVFFFFSRREIKKNLGWRKMLVFLGIILLIYLPV
                     VANEIITHGKDTAAFFAAVKSKPSHHSLRENVIRNVQYWGQYWFLIATSWIGKESGFV
                     SSVVAWVFLMLPGMFLAIKYYREEKNNLRKKFLLITVLWFSAYFLIYIPIAYQIRPRF
                     FLPMLALPFVFAGYIAKYLWERNNKFLKAAVAVFLAIIFFGNLAGTYLWFKEIKDAQK
                     KGTYPNRTIILKARDGIVLWHLENAENYIREDCQENTAYYYATNEYKRPVEYLLGLQG
                     IRGATIDNLYLGQKNCVYGVLLTRSAKTKLPASITDKFDNVDSKKFGAITVYKLSAKD
                     EFVGQPLGYTKKPLPEQSLESGRIFWKDFFNTDKKQKAKK"
     gene            1201..1935
                     /locus_tag="VMQ48_01630"
     CDS             1201..1935
                     /locus_tag="VMQ48_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08773.1"
                     /translation="MKNTKISVVTPCFNEGKNIRKNIKRVDDYLKQRFDRYEIIAVND
                     GSRDNTADELLALQQEIGLKFINNKENQGKGGAVRDGMLATSEGSDVAMFLDSDLGIP
                     IEELEKFVAEIRNGHDIVIASRFVPGLKIIQSVQLHRKLMEKAFRLIRMVITNNWNVK
                     DTQCGFKVFRRQAAMKIFPKLTVKRFAFDAEVIFVANKCGYKIKELPIHLQNPPSRSL
                     RIFRDPLNMVLDLLKIRKNNWQKKYE"
     gene            1928..>3142
                     /locus_tag="VMQ48_01635"
     CDS             1928..>3142
                     /locus_tag="VMQ48_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08774.1"
                     /translation="MNRILKNEKFRVVLFFTALTVILTWPSVWHFFSSVPSSGADTMQ
                     VIGVAGDKANLLSDQGIFKGTIELIKRSEFNITTLYAYFQLIFGRAVGYNILFFASFI
                     LSGFGAYLLAFYFTKNKPASLIAGIIFAFSPFHIYNSLSTNAGTMHQEWLPFFALYLF
                     KFFEDLNWKNFSLAGLFLLLIGFTEHQLLAFTVIFILFFLVYKLIIQPKIYLRGKFWA
                     YAGTSILVLSIVFFFMFRSLFVIAGSDNNFLDAGFKSAVKYSNDALSIFVPPNLHTFW
                     PDTFSHLRDQFERNSDSTFSAYAGYSVLLLSLLGIFGIRFLKKKNIPTRGIFFWFFVA
                     VGFYVLSWGPYLHYKGLLDPPVKMPYYFIYQYLPFYKNIRTVGRLFVWSILGFSMLAA
                     WGMTYINFQFSIF"
BASE COUNT          842 a          651 c          723 g          926 t
ORIGIN      
        1 ttctgtcgtt tgcgactttg ccggtaatca cggttgtttt cttctttttc agccgcaggg
       61 aaatcaaaaa aaatttgggt tggagaaaga tgctggtctt tttgggcatt atcttgctta
      121 tatatctgcc ggttgttgcc aacgagatca taactcacgg aaaggacacg gccgcatttt
      181 tcgcggctgt caaaagcaag ccctcgcatc acagcctccg ggagaatgtc attcgaaatg
      241 tgcagtattg ggggcagtat tggtttctga tcgcgacaag ctggatcggc aaagagagcg
      301 gttttgtttc gtccgtcgtt gcgtgggtat ttttgatgtt gccgggaatg ttcctcgcga
      361 taaaatatta tcgcgaggaa aaaaacaatc ttcgaaaaaa attcctgctc atcacggttc
      421 tgtggttttc agcgtatttt ctcatctata ttccgattgc ctatcagatc cggccgcggt
      481 tttttctgcc aatgctggct ttgccgtttg ttttcgccgg atatattgcg aaatatcttt
      541 gggaaagaaa caacaagttt ctgaaagctg cggtggcggt attcctggcg ataatatttt
      601 tcggaaatct ggcggggaca tatctgtggt tcaaagaaat caaggatgct cagaagaagg
      661 gaacttatcc aaataggacg attattctca aggcgcgcga cgggatagtc ttgtggcacc
      721 tggaaaacgc tgaaaattat atccgcgagg attgccagga aaataccgca tactattatg
      781 caaccaatga atacaaaagg ccggtggaat atttgctggg attgcaaggt atccgaggag
      841 ccacgattga taatctgtat ttgggacaga aaaactgtgt atacggcgtg ctcttgaccc
      901 gtagcgcgaa aacgaaattg ccggccagta taaccgataa gtttgacaat gttgattcca
      961 aaaaattcgg agcgatcaca gtctacaagt tatcggcgaa ggatgaattt gtcggacagc
     1021 cgcttggata tacaaaaaag cctcttccgg aacagtcgct tgagtctggg cgaatttttt
     1081 ggaaagattt ttttaatacg gacaagaaac aaaaagcaaa gaaataaggg gagtgagaga
     1141 aactctggaa aaattgagga aaatatagta gattaataaa tgtgagatcg agaggaatat
     1201 atgaaaaaca cgaaaatatc cgtagttact ccctgtttca atgaagggaa aaatatccgg
     1261 aaaaatatca aaagggtcga cgattatttg aagcaaagat ttgatcgcta cgaaataatc
     1321 gccgtaaacg acggaagccg cgacaatacg gcggatgagc tcttggcgct tcagcaagaa
     1381 attggattga aattcattaa taataaagaa aatcagggca aaggaggcgc agtgagagat
     1441 ggaatgctgg caaccagcga gggaagcgac gtcgcgatgt ttcttgattc ggatcttgga
     1501 atacccattg aagaacttga aaaatttgtc gcggaaattc ggaacggcca tgacattgtc
     1561 attgcttccc ggtttgttcc cgggctcaag atcatccagt ccgtgcagct ccaccgcaag
     1621 ctgatggaaa aagcttttcg tctgatcagg atggtcatca ccaacaactg gaacgtgaaa
     1681 gacactcaat gcgggttcaa agtttttcgg cgccaagccg cgatgaaaat tttccccaag
     1741 ctcaccgtga aaagattcgc ttttgacgca gaagtcatct tcgttgcgaa caaatgcgga
     1801 tataagatca aagaacttcc gatccatctc cagaatcctc cctcaagatc cctccggata
     1861 tttcgcgatc cattgaacat ggttttggat ctcctaaaga tcagaaaaaa taattggcaa
     1921 aaaaaatatg aatagaattc tcaaaaacga aaaatttagg gtcgtattat ttttcacagc
     1981 gttgaccgtg attctcactt ggccttcggt ttggcatttt ttctcgtccg tgccgagttc
     2041 cggagcggat acgatgcaag tgatcggtgt ggcaggggac aaggcaaatt tgctttccga
     2101 ccaaggtatt ttcaaaggga caattgagct catcaagcgg tctgaattca atatcacgac
     2161 attgtatgcc tattttcagt tgatctttgg ccgggcagtt ggctacaata ttttgttttt
     2221 tgcgtccttc attctttctg gattcggtgc atatcttttg gccttttatt tcaccaagaa
     2281 caagccggct tcgctcattg ccgggatcat ttttgctttt tctccgtttc atatctacaa
     2341 ctcactatcc accaatgccg gaacaatgca tcaagaatgg cttccttttt tcgcactgta
     2401 tcttttcaag ttttttgaag acctaaattg gaaaaatttc tctcttgccg gtttgttttt
     2461 gcttctgatc ggtttcacag agcatcagct tttggccttt acggtcattt ttatcttgtt
     2521 ttttcttgta tacaaactca ttatccagcc gaaaatatat ttgcgcggaa aattttgggc
     2581 ctatgccgga acgagcattc tggtcttgtc aattgtgttc ttttttatgt tccgatccct
     2641 ttttgtgatt gccgggtccg acaacaactt tctcgatgcc ggattcaaat cagccgtgaa
     2701 atattcgaac gacgcgctca gtatttttgt gccgccgaat cttcacacct tttggccgga
     2761 cactttttcg cacctccgcg accaattcga gcgcaactcg gacagcactt tttcggccta
     2821 tgccggctat tccgtgctct tgctttcgct cttgggaatt ttcgggataa gatttctcaa
     2881 aaagaaaaac attccaacca ggggaatatt tttctggttc ttcgtggcag tcggctttta
     2941 tgtgctgtcg tggggaccgt atctgcatta caagggactt cttgatccgc cagtcaagat
     3001 gccgtattat ttcatctatc aatatttgcc tttttacaag aatatccgga cagtgggacg
     3061 gcttttcgtc tggtcgatac tcgggttttc aatgctggcg gcgtggggaa tgacatatat
     3121 caattttcag ttttcaattt tc
//
LOCUS       DATKGS010000068         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_131791, whole genome shotgun sequence.
ACCESSION   DATKGS010000068 DATKGS010000000
VERSION     DATKGS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131791"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VMQ48_01640"
     CDS             <1..226
                     /locus_tag="VMQ48_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08775.1"
                     /translation="IQNMMQQLPGQTGATGTDGTNGAANVPGTDNSVGNTLPAPPNEN
                     LQNPADSTNNNPPMNSSAPDQKKNGVPQLP"
     gene            239..>2559
                     /gene="gyrA"
                     /locus_tag="VMQ48_01645"
     CDS             239..>2559
                     /gene="gyrA"
                     /locus_tag="VMQ48_01645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUD08776.1"
                     /translation="MEIGYIKSRELTTEMKESYLAYAMSVIVSRALPDVRDGMKPVHR
                     RILFAMRGLGLTHSAKYRKSALVVGDVLGKYHPHGDLSVYDAMVRLAQDFSMRYPLVD
                     GQGNFGSMDGDSPAAMRYTEAKMTSLAEEMMADIEKETVDFVPNYDASRQEPSVLPAK
                     LPQLLLNGTMGIAVGMATNIPPHNLGELVDAIAYLIENPDCDVKDLTQFVKGPDFPTG
                     GSIYNQADIMQAYLTGRGSIVCRAETEIVETKSGQFQIIVTEMTYSSNKAAMIEKMAD
                     LVKTGRIEGIKDLRDESDKDGVRVVIELKKEAFPKKILNKLYTLTDLQKSFHMNMLAL
                     VDGIEPKVLNLKAMLEYFIAHRQVVVTRRTQYDLRIAKERAHILEGLKKALDHIDAVI
                     NTIRKSATKEIAHDNLMKKFKLSDKQSTAILEMRLQTLAGLERQKIEDELKEKMKLIK
                     ELEELLKSPKKILSVIKTELQELKDKYADERRTKVVRSAVGEFSQEDLIPNEEAIIVL
                     TEDNYVKRMNPSLYRVQRRGGKGVIGATTKEDDKIDMVVSAMTHDDLFFFTNTGKVFQ
                     TKAYEIPVSSRTAKGQSIVNFLQISPDEKITAIITVGKDSQAKYLFMATKFGTVKKTA
                     IEDFAKVRRSGLIALNLAAGDELRWIAPTTGKDEIIITTSAGQAVHFKETDVRGMGRT
                     AAGVRGINLKKNDAVVGMDVVLKNQKGNQLLVLTENGFGKRSDMKAYKIQRRGGSGIK
                     TAKVTPKTGKLVCAHVVNLDNLEADLIASSANG"
BASE COUNT          759 a          591 c          675 g          534 t
ORIGIN      
        1 gatccaaaat atgatgcaac aattgcccgg ccagacgggc gcgacgggaa cggatggtac
       61 gaatggcgca gctaatgttc cgggaacgga taatagcgtg ggaaatactt taccggctcc
      121 ccccaatgaa aatttgcaaa atccagccga tagcacaaat aataatccac caatgaatag
      181 cagtgctccg gatcagaaaa aaaacggtgt gccgcagcta ccctaaccag aaaaaaatat
      241 ggaaattgga tacatcaaat caagagaact gactaccgag atgaaagaat cctatctcgc
      301 ctacgcgatg agcgtgattg tttcgcgtgc tcttccggac gtgcgggacg ggatgaagcc
      361 ggtgcatcgc cggattcttt tcgcgatgcg cgggcttgga ctcacccaca gtgcgaaata
      421 cagaaagtcg gcgttggttg ttggagacgt gctgggaaaa tatcatccgc acggtgattt
      481 gtcggtttat gacgcgatgg tgcggcttgc ccaggatttt tcaatgcgct accctcttgt
      541 ggacggtcag ggaaacttcg gatcgatgga cggcgacagc ccggcggcga tgcggtatac
      601 cgaagccaaa atgacgtcgc ttgcggaaga gatgatggcc gatattgaaa aagaaacggt
      661 ggatttcgtc ccgaactatg atgcgtcgcg ccaggagcca agtgttcttc ctgcgaaact
      721 tccccagctt cttctcaatg gcaccatggg catcgcggtt ggtatggcaa cgaatattcc
      781 gccgcacaat cttggcgaat tggtggatgc cattgcctat ctcatcgaga atccggattg
      841 cgacgtgaaa gacctcaccc agttcgtgaa aggcccggac tttccgacgg gcggatcgat
      901 ctataatcaa gctgatatca tgcaggccta tctcaccggc cgtggaagta ttgtttgtcg
      961 cgccgagact gaaattgtcg agacaaagag cggccagttc cagatcatcg tgacggaaat
     1021 gacctattca tcgaacaagg cggcgatgat cgagaaaatg gcggatctcg tgaaaaccgg
     1081 aagaatcgaa gggatcaaag acctgcgcga cgagtcggac aaggacgggg tgcgagtggt
     1141 gattgagctg aaaaaagaag ctttcccgaa aaaaattctc aacaaactat ataccctcac
     1201 tgatcttcaa aaatcgtttc acatgaatat gctggctctg gtcgacggaa tcgagccaaa
     1261 agtcttgaat ctcaaggcga tgcttgagta tttcatcgcc catcgtcagg tggtcgtcac
     1321 gcgccggaca cagtatgacc tgcgaatcgc aaaagaacgg gctcacatct tggaaggact
     1381 caaaaaagcg ctcgatcata tcgatgccgt catcaacact atcagaaaat cagcgacgaa
     1441 agaaattgct cacgacaatt tgatgaaaaa attcaaatta tcggacaaac agtcaacggc
     1501 catcttggaa atgcgtctcc aaactctcgc cgggcttgag cgtcagaaaa tcgaagacga
     1561 actcaaggaa aaaatgaaat tgatcaaaga gctggaagaa cttttgaaga gtccgaagaa
     1621 aattttgagt gtcatcaaga cggaactcca ggagctcaaa gataaatatg ccgatgagcg
     1681 ccgcacaaaa gtggtgagat cagcggtggg tgaattcagc caggaagatc ttattccaaa
     1741 cgaagaagcc attattgttt tgactgaaga caactatgtg aaaaggatga atccctcact
     1801 ctatcgtgtg cagaggcggg gcgggaaagg cgtgatcggt gcgaccacca aagaggacga
     1861 caaaatagat atggtggtga gcgcaatgac gcacgatgat cttttctttt tcacgaatac
     1921 gggaaaagtt tttcagacca aagcctatga gattcctgtt tcttctcgga cagccaaggg
     1981 tcagtcaatt gtgaactttt tgcaaatttc tccagacgaa aaaatcacag cgattatcac
     2041 tgtcggaaaa gatagccagg cgaagtatct tttcatggcg acgaaattcg ggacagtgaa
     2101 aaaaacagcc attgaagatt ttgccaaagt tcgccgatcc ggtctcatcg ctttgaacct
     2161 cgctgcgggc gacgaactgc gctggattgc tccaacaacc ggaaaagatg aaatcatcat
     2221 cacaacttca gccgggcaag ccgtccattt caaggaaacg gacgtgcgcg gcatgggtcg
     2281 gacagcggcc ggggtacggg gaatcaatct caagaagaac gatgctgtag tcggaatgga
     2341 tgtggtgctc aagaaccaga aaggcaacca gcttttggtg ctgacagaaa atggattcgg
     2401 caaaagatcg gatatgaaag cctacaaaat tcagcggcgc ggcggcagcg ggatcaaaac
     2461 ggcgaaagtc actcccaaaa ccggcaaatt ggtgtgcgcg catgtagtca atctcgacaa
     2521 tcttgaagcc gatctcatcg cgagctccgc caacggcca
//
LOCUS       DATKGS010000069         8467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_164424, whole genome shotgun sequence.
ACCESSION   DATKGS010000069 DATKGS010000000
VERSION     DATKGS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164424"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VMQ48_01650"
     CDS             complement(<1..613)
                     /locus_tag="VMQ48_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUD08777.1"
                     /translation="MIGRKGAICYHSSMKNIQEKFDVAVIGGGPAGMMAAGRAAELGA
                     NVVLLEKNESLGKKLLITGKGRCNLTQAQFDLKEMVSQFGKNGKFLFSSLAEFGPREV
                     IKFFEDRKLSTKTERGARVFPVSNKSYDVLRVMEEYLKKNKVKILFGSEIVGFESDGK
                     KIKGVKLKHEKGAPGRKIISADRFILCTGGKSYPTTGSTGDGSP"
     gene            complement(620..1309)
                     /locus_tag="VMQ48_01655"
     CDS             complement(620..1309)
                     /locus_tag="VMQ48_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08778.1"
                     /translation="MDKSKIHFVLVAGFSSDHLEAMGLKKSLEKKGFSADAISFYGDG
                     YIDDFTDLKISDCIANIAEFINGRAGQYETVFGIGISLGGALLLEHAKSYNNLKGIAS
                     IGTPLKLKNRVLISFGQKILPLVYLFWRRMQKIKRLRLNPLGATNMMIEYLETGLPKN
                     LDYIKTPVLLLHSKKDPVSDYRVLPKYLNLISSKKKRITFFDNGNHVIDHDPDLVVKY
                     ALDFLEINVRK"
     gene            complement(1403..3178)
                     /locus_tag="VMQ48_01660"
     CDS             complement(1403..3178)
                     /locus_tag="VMQ48_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUD08779.1"
                     /translation="MNYSLTKTDVEKEKEKSVLSTAWRRLVPLMAEEKRSVMIAIIAI
                     LVSSAATLVVPIIIAHIVDVYMVNKDFHGVLLFSGLLFVVFLSGLASSYVQVKTMGGV
                     GRRLLFNLRNKIFNKLQALPVAFFNQNKAGDLISRINNDTDKLNQFFSQALMQFFGNI
                     ILIIGAGIFLIAIYWRLGLVALVPAVIALIATQLLSPWVKRTSLASLRTLGAMSGEIQ
                     ESINNFKVIVAFNRLDYFRNKFKEANETNYSASVSAGIAGNVFNPIYVLASSLAQLSV
                     LAYGIYLISAGHFTVGLLIGFLLYVNSFYNPLRQLASVWPSLQLALAGLDRISEVLAL
                     ESDIKTMPKETAEAFSSSNAVLEFKNVFFNYEGGKDVLKNINFSLERGKTYALVGPTG
                     GGKTTTASLMARLYDPSKGTIYLEGKDIRLYKPGERVKKIGFILQEPFLFNGTVRENI
                     LYGNEEHQGYTKDELAQVLEKAHLTKLLSRFSNGLDTQVSSSGDAISLGQKQLIAFIR
                     ATLRNPDILILDEATANIDTVTEQLLQEIIDKLPEKTTKVIIAHRLNTIENADDIFFV
                     NAGEVTHAGSMQQAVDMLLHGERRS"
     gene            complement(3197..4951)
                     /locus_tag="VMQ48_01665"
     CDS             complement(3197..4951)
                     /locus_tag="VMQ48_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUD08780.1"
                     /translation="MQQRKKQKGQNNDMSLPAPSLFGLLRRYAGIISMLIVLTIIANA
                     LSISVPKIIAGAIDTYTNGNFFITHLIVEFSIIAFLIFVFTYLQNIVQVLASERVARD
                     MRNDIAAKISVQPYSYIESATPAMLLTNLTSDVDAVKTFVSMAVSSIISSIFLIIGIS
                     VLLLVTDWKLGLAVLSVVPFIGVTFFFVLTKVRKLFVKSQEAIDWLNKVISESILGAA
                     LIRLLNSKKPEYKKFHAANTEAKRVSLQILGLFASLVPVITFFTNIATLAIVLLGGHF
                     IIQGTMSLGNFTAFNSYLAILIFPILIIGFMSNVIAQAQASYGRILQVLNAPIEKKTG
                     GLTVALKGDIEVKNVTVKFGEKSALKDVSFSVKAGSKTAIIGPTAAGKTQLLYLLTGL
                     LAPTSGEIDFDGKDINEYDKESLHSQVGFVFQDSVMFNLSLRENIAFSNTVKDENIEK
                     AIATAELRDYIDALPQKLDTIVSERGTSLSGGQKQRIMLARALALNPKILLLDDFTAR
                     VDANTESKILSNIAKNYPGLTLISVTQKISSIERYDQIVLLMEGELLAKGTHEELMQT
                     SPEYVQIYQSQRSTSQYE"
     gene            complement(4962..5096)
                     /locus_tag="VMQ48_01670"
     CDS             complement(4962..5096)
                     /locus_tag="VMQ48_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08781.1"
                     /translation="MEKLRSKRIQDMEHVFDIFTDAEFEAYCRLHEKIAESLAVRKKQ
                     "
     gene            complement(5143..5628)
                     /gene="msrA"
                     /locus_tag="VMQ48_01675"
     CDS             complement(5143..5628)
                     /gene="msrA"
                     /locus_tag="VMQ48_01675"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HUD08782.1"
                     /translation="MLKKIVLGGGCFWGLQELLRKQPGVVKTYAGYTGGKVKNPTYEN
                     HEGHAEAVEIEYETEKTSYKKILDFFFRVHDPTTLNQQGNDRGTSYRSVIFYGNDEEK
                     EKAEDFIDIVNKSDRWKDPVATTLEPLKKFYPAEDYHQNYLQKNPDGYTCHAIRFGSY
                     L"
     gene            complement(5673..6380)
                     /locus_tag="VMQ48_01680"
     CDS             complement(5673..6380)
                     /locus_tag="VMQ48_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUD08783.1"
                     /translation="MSQKISTKLLIAAIAILAFVAIFLVWQSEKKNSELISQQKTQAT
                     QLGGVEVREYNGEKLSSIGDVVDQGIKGVQNIDVKNYTLQMTGLVDQPKNFTYDQVLQ
                     LPKYKKAVDLNCVEGWSAKILWEGVQVKDLIDSAGPKAEANTVIFYAQDGYTTSLPLD
                     YIQNNNILLAYKMNDVILSPAKGFPFQLVAEQKWGYKWIKWVTKIELSNDPNYKGYWE
                     SNGYNNNGDVKGSKFAP"
     gene            complement(6610..7026)
                     /locus_tag="VMQ48_01685"
     CDS             complement(6610..7026)
                     /locus_tag="VMQ48_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HUD08784.1"
                     /translation="MKIKSITILEVEYIAYRLAKETMKWDEPIPDFGSRFPGKLESCL
                     AVPFQSFGGKLFYKGLIGKASILFYLMVKNHPFENGNKRIAMTALLHFLRKNKKWLQV
                     DNQELYNFSRWVAESNPKLKEETVAAIRKFLSTYLV"
     gene            complement(7023..7277)
                     /locus_tag="VMQ48_01690"
     CDS             complement(7023..7277)
                     /locus_tag="VMQ48_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUD08785.1"
                     /translation="MDKKYLTTSELAKLLGISRIAVFKKIKTGKIKAKKIGRNFVIDK
                     KELGGILDESLTERKKIEISETVKKTIKEYGETLKLLGKE"
     gene            complement(7526..7867)
                     /locus_tag="VMQ48_01695"
     CDS             complement(7526..7867)
                     /locus_tag="VMQ48_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08786.1"
                     /translation="MPLLSEEAHPIPGLGFLSSLWLPSRKQTEAARQELAGSNEKDFI
                     FAAHPLTQEFAEGKLNIGTRVKIDGRDLVLKGVYPKAEYPYAMVFWDAVKGCYIQFHF
                     LPCYSDHEILN"
     gene            complement(7914..8132)
                     /locus_tag="VMQ48_01700"
     CDS             complement(7914..8132)
                     /locus_tag="VMQ48_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08787.1"
                     /translation="MQLKKRLLKFFGIGLTKEEKIARAKRKYKDWELVSKIMEIENPP
                     DDEARYYLLIDLCAEYQIDFVIPCSGPG"
BASE COUNT         2319 a         1935 c         1689 g         2524 t
ORIGIN      
        1 ggggggatcc atcaccggtc gaaccggtgg ttggatatga ttttccgccc gtacacaaga
       61 taaatctgtc ggccgaaatt atttttctgc ccggagcgcc tttttcgtgt ttcaatttca
      121 cgccttttat ttttttccca tcactttcga atccgacaat ttcactgcca aacaatattt
      181 tcactttgtt ttttttcaaa tattcctcca tcactcgcaa aacatcatat gatttgttgc
      241 tcaccggaaa aacccgcgcg cctctttcgg tttttgtgga caattttctg tcttcaaaaa
      301 attttatcac ttctctcggc ccaaattccg cgagcgagga aaacaaaaac tttccgttct
      361 tgccgaattg actgaccatt tctttgaggt caaactgcgc ttgcgtcaaa ttgcagcggc
      421 ctttgccggt gatcaaaagt tttttgccga gactttcatt tttttccaaa agcaccacgt
      481 ttgctccgag ctctgccgcc cgtcccgccg ccatcatgcc ggccggtccg cccccgatga
      541 ccgccacatc aaatttttcc tggatgtttt tcatggaaga atggtagcat attgcgccct
      601 ttcgtccaat caaaaaatat tattttctaa cgttaatttc gaggaagtcc aaagcgtatt
      661 ttacgacaag atcaggatca tgatcgataa catgatttcc attgtcaaaa aatgtgatcc
      721 tctttttttt acttgaaatc agattcaaat atttcggaag aacccgataa tcagataccg
      781 ggtctttttt gctgtgcagg agcagaaccg gagttttgat atagtcgagg ttcttgggaa
      841 gacctgtttc caaatattca atcatcatat tcgtagcccc aagcggattc aggcgcaagc
      901 gcttgatctt ttgcatccgc ctccaaaaaa gatagacaag cggcaaaatt ttctggccaa
      961 atgatatcag gaccctgttc ttcaatttaa gaggcgtacc gatgctcgct atccccttca
     1021 aattgttata gcttttcgcg tgttcaagaa gcagcgctcc acccaggctg atgccaatac
     1081 caaaaactgt ttcatattgc ccagcgcgtc cattgataaa ctccgctata tttgcgatgc
     1141 aatcggaaat tttcaggtcg gtaaaatcat cgatataacc atcgccataa aagcttattg
     1201 cgtctgcgct gaatcctttt ttttccaaac ttttcttcaa ccccattgct tccaagtgat
     1261 cagaagaaaa accggctacc agcacaaagt ggatttttga tttatccata ctcggcaaat
     1321 ataatgaatt gcgaaaataa atgctcctga catcaatttt accacaaaca ggacactaat
     1381 cctacaatga caacaaatca gactagctcc ttctttctcc gtgcaagagc atatccactg
     1441 cctgctgcat agatcccgca tgagtgactt caccagcgtt tacgaagaat atgtcgtcag
     1501 cattctcgat ggtattgaga cggtgtgcga taataacttt cgtcgttttt tccggcaact
     1561 tatcaatgat ttcctggagc agctgttctg taacggtatc tatattcgcc gtcgcttcat
     1621 ccaaaatcaa gatgtccgga ttgcgcagtg tcgctcgaat gaatgcaatc aattgttttt
     1681 gccccaaact aatagcatcc ccgcttgaag agacttgggt atcgaggccg ttagaaaatc
     1741 gcgagagcaa ttttgtcaga tgcgcttttt ccaacacttg tgccagttca tctttggtgt
     1801 atccttgatg ctcctcattt ccatataaaa tattttcgcg caccgtgccg ttaaagagaa
     1861 acggttcttg caggataaat ccgatctttt ttactcgttc gcccggttta tataaacgaa
     1921 tatctttccc ttccagataa attgtacctt tgctcgggtc ataaaggcgt gccatcaaag
     1981 acgctgttgt tgtttttccg ccgccagtcg gcccgacaag cgcatatgtc tttcctctct
     2041 cgagtgaaaa attaatattc ttcaaaacat cctttcctcc ttcataattg aaaaatacgt
     2101 ttttaaattc aagtacggcg ttggatgaag aaaaggcttc cgctgtttct ttcggcattg
     2161 ttttaatatc agattcaagc gccaatacct cggaaatacg atccaagccg gctagggcaa
     2221 gctgaagtga tggccacaca gacgcaagtt gtcggagtgg attgtaaaaa ctgttgacat
     2281 atagcaaaaa tccaataagc aggccgaccg tgaaatgacc cgcggatata agatagattc
     2341 cataagctaa aacggaaagc tgcgccaaag acgaggcaag cacatatatg gggttgaaaa
     2401 cattgcctgc gataccggcc gaaacagatg cggaataatt tgtctcgttg gcttctttaa
     2461 acttatttct gaagtaatca aggcggttga atgccacaat cactttgaaa ttgttgatgc
     2521 tttcttggat ttctccgctc atagctccca gtgtgcgaag tgacgccaaa ctggtgcgct
     2581 tgacccaagg agaaagcagc tgagtggcga tcaaggcgat gaccgcaggc acgagtgcaa
     2641 caagaccgag tcgccaataa atagcaataa ggaaaatacc ggcaccgatg ataaggatga
     2701 tgttgccgaa aaattgcatc aatgcctgtg aaaaaaattg attgagcttg tcggtatcgt
     2761 tgttgatgcg cgaaataaga tcgccggcct tgttttgatt gaagaaagca acgggaagcg
     2821 cctgaagttt gttgaatatt ttgttgcgca aattaaagag caatcttctg cctactcctc
     2881 ccatcgtttt gacttgcaca taacttgaag caagtcccga aagaaaaaca acaaacagca
     2941 gccctgaaaa caataacact ccgtgaaaat ctttattgac catgtagacg tctacaatat
     3001 gagcgataat tatcggcacg accagcgtcg cagccgagct cactaatatg gcaattattg
     3061 cgatcatcac tgaacgtttt tcttctgcca tgagaggcac aagcctgcgc cacgcggtgg
     3121 acagcacgct tttttccttt tctttctcaa cgtctgtttt agtaagagag taattcatat
     3181 atcttttttc cttattttat tcatattggc tcgtgctccg ctgcgactga taaatttgga
     3241 cgtattcggg cgacgtttgc atcaattctt catgggtgcc ttttgcaagc aattcccctt
     3301 ccatgaggag aacaatctgg tcataacgct caatagacga aattttctgg gtaacggata
     3361 tgagcgtgag tccgggataa tttttggcaa tattgcttaa aatcttgctt tcagtattgg
     3421 catcaacgcg cgcggtaaaa tcatccagga gcaatatctt cggattgagc gccagcgcgc
     3481 gcgccagcat aatgcgctgt ttttgtccgc cggaaaggct ggttcctctt tctgaaacta
     3541 tcgtgtctaa tttttgtggc aaggcatcaa tgtaatcccg aagctcggca gtcgctatag
     3601 ccttctcaat attttcgtct ttaactgtat tgctgaatgc aatattctcc cgcaaactca
     3661 aattaaacat gacactgtct tgaaacacaa atccgacctg gctgtgcaaa ctttctttgt
     3721 cgtactcgtt gatgtctttg ccgtcaaaat cgatttcgcc cgacgtggga gccaaaagac
     3781 ccgtaagtaa atagaggagc tgggttttgc ccgcggctgt cggaccgata atggcagtct
     3841 tgctgccggc ttttacggaa aaagaaacat ccttgagcgc cgatttttct ccaaatttaa
     3901 ccgtcacatt tttcacttca atgtctccct taagcgccac cgtgagtccc cccgtcttct
     3961 tttcaatcgg cgcattgagc acttgcagaa tgcgtccgta tgaagcttga gcctgggcaa
     4021 tgacgttgct cataaacccg ataataagta ttgggaaaat aaggatggcc agataactgt
     4081 tgaaggcagt gaaattgccg agcgacattg ttccctgaat gataaaatgt cctcccaaaa
     4141 gaactatggc cagtgtcgca atgttcgtaa aaaatgtaat gacagggaca aggctggcga
     4201 acaaccctaa tatttgaaga ctgacccgtt tcgcttcggt attggcagcg tgaaattttt
     4261 tgtactcggg ctttttagaa ttgagcaagc ggatgagagc tgcgcctaaa atgctttcgc
     4321 ttatgacttt attgagccag tctatcgctt cctgtgattt tacgaagagt ttgcgaactt
     4381 tggttaatac aaaaaagaaa gtcactccga tgaaaggcac gactgaaagc acggcaagtc
     4441 cgagcttcca atcagtgaca agcagcagca ccgatatgcc aataatcaaa aatatggatg
     4501 aaataatgga cgaaacggcc attgatacga acgtctttac cgcatcaacg tctgaagtaa
     4561 gattggtcaa aagcatcgcg ggtgtagcac tttcgatata tgaatacggt tgcactgata
     4621 tcttggctgc aatatcgttg cgcatatcgc gggctactcg ctcggaagcc aatacctgca
     4681 caatattttg cagataagtg aatacgaaaa taagaaatgc aataatagag aattcgacga
     4741 tgagatgcgt gatgaaaaaa ttgccgttcg tgtaagtatc aatggcgcct gcaatgattt
     4801 tcgggactga aatgctcagc gcattagcaa taatagtaag cactataagc atactgataa
     4861 tacctgcgta ccgacgcaac aaaccgaaaa ggctcggcgc tggcaagctc atgtcattgt
     4921 tttgaccttt ttgttttttt cgctgttgca taataaattg gttattgttt ttttctaacc
     4981 gccaggcttt ctgctatttt ttcatgcaag cggcaatacg cttcaaattc cgcgtcagtg
     5041 aaaatgtcaa aaacatgctc catatcctgg atgcgcttac ttctaagctt ctccatgcgt
     5101 tttttgcttt tgtctgaaag tgaaagactg agttagcacc cttcacaaat aactgccgaa
     5161 ccttatggcg tggcaagtgt acccatcagg attcttttgc agataattct gatgataatc
     5221 ttccgccgga taaaattttt tcaagggctc caaggtcgta gcaacgggat ctttccagcg
     5281 gtcggattta ttcacaatat ctataaaatc ttcagctttc tctttttctt cgtcgttgcc
     5341 ataaaatatc actgaacgat aagacgttcc acgatcattg ccttgctgat ttagggttgt
     5401 tggatcgtgc acgcgaaaga aaaaatccag tatttttttg taagaagttt tttctgtctc
     5461 atattcgatc tctaccgctt ctgcgtgtcc ttcgtgattt tcataagttg gatttttaac
     5521 cttcccgcct gtataacccg catatgtttt caccacccca ggctgtttcc gcaggagttc
     5581 ctgcaatccc caaaaacaac ctcctcccaa tactattttt ttaagcataa aattatttga
     5641 tttaacgaaa tcatgccgcg caaccgccca ctctatggcg cgaatttgga acccttaaca
     5701 tcgccattgt tattataccc gttgctttcc caatatccct tgtaatttgg atcgttggac
     5761 aattcaattt tcgtcaccca tttgatccat ttatatcccc acttttgttc cgctaccagc
     5821 tggaaaggaa atcctttggc cggagacaag atcacatcat tcatcttgta agccagaaga
     5881 atattattat tttgaatata atccaaaggt agggatgtcg tatagccgtc ctgggcataa
     5941 aagatgaccg tgttggcttc ggctttcggc ccggctgaat caataaggtc tttgacctgc
     6001 acgccctccc ataatatttt ggcgctccag ccttccacgc agttcaaatc aaccgccttt
     6061 ttatattttg gaagctgcaa aacctgatca tacgtaaagt ttttaggctg atcaacaagg
     6121 ccggtcattt gaagggtgta gtttttcacg tcgatatttt ggactccttt gattccctga
     6181 tccacaacat ctccaatgga ggacaatttt tctccgttgt attctcgtac ctcaactccc
     6241 cccagctgag tggcttgcgt cttttgctgg gaaattagct cgctattttt tttctccgat
     6301 tgccaaacaa gaaaaattgc gacaaaagcc aaaatggcta tcgcagctat caataatttt
     6361 gttgaaattt tttggctcat atttttttaa ctattatatt tttattttac cacggaaaat
     6421 actaacttcc tatctgaaca acaaaaataa ggcttatata agccttaaaa aaacgtgact
     6481 cgcttggcag gacagctttc gaacttttga ttgggaaaag gcctttcccg atccggtaat
     6541 tgttatcagc caaacgagaa gattattggc acttacataa aataaaaaac agcgtagtga
     6601 aatagttttc taaaccaaat aggtgctcag aaatttccga attgctgcga cagtctcttc
     6661 tttgagcttc ggattgcttt cggcgaccca tctggaaaag ttgtaaagtt cttgattatc
     6721 aacctgaagc cattttttat tcttacgaag aaaatgcaaa agagcagtca tggcaatcct
     6781 cttgttgcca ttttcgaaag gatgattttt caccatcaaa taaaacaaaa tggaagcttt
     6841 tccaataagg cccttataaa acaattttcc gccaaaagac tgaaagggaa cagctaagca
     6901 actttccagt tttccgggaa aacgtgaacc aaaatcaggg ataggctcgt cccacttcat
     6961 tgtttctttg gccagtctat aagcaatgta ttcaacctcc aatattgtga tggattttat
     7021 tttcattctt tgcccaataa tttcaaagtt tcaccgtatt ccttaatagt ctttttcacc
     7081 gtttcgctga tctcaatctt ttttctttcc gtgagcgatt cgtcgagaat tccgcccaat
     7141 tcttttttat caataacaaa attccggccg attttcttgg cttttatctt tccagtcttg
     7201 atttttttga aaacagcaat ccgactgatg cccaaaagct tggctaattc agatgtcgtt
     7261 aaatattttt tatccataac ccaattttaa tctatgttaa ccaatgtgtc aatggttaac
     7321 aaaaatcagg ctttatacaa gcctgaaaaa cgttgctcgc ttggcgggac agctttcgaa
     7381 catttgagtg ggagaaagct ttccctgatc cgatgattgt gatcaatcag acgaagcaac
     7441 ttttggagct ggcttgaaaa tttggaaata aaaaagacca gattacggac tggtctgtcg
     7501 gcactattgt gccccgaaga gattgtcagt tgaggatttc gtggtcgctg tagcacggca
     7561 gaaaatgaaa ctgaatgtag catcccttga cagcgtccca aaagaccata gcatagggat
     7621 actcggcttt cggatagacg cctttcagaa cgaggtctct gccgtcaatc ttcaccctcg
     7681 tgccgatgtt caacttacct tcggcaaatt cttgggtgag cggatgagcc gcaaagatga
     7741 aatccttttc gttcgacccc gccaattcct gcctggccgc ctctgtctgt ttacgcgagg
     7801 gtagccagag agaagacaag aatccgagtc cgggaattgg gtgtgcttct tcgctcaata
     7861 atggcatagt tatcacctcc gtctttgctc atatgtactt ttcaaagaaa ggtttatccc
     7921 ggcccactgc aaggaatgac gaaatcgatc tggtattcgg cacaaagatc aatcaagaga
     7981 tagtaccgag cttcgtcatc aggcgggttc tcaatttcca taatcttgct cactaattcc
     8041 cagtctttgt actttctttt ggccctggcg attttttcct ccttcgtcag gccgataccg
     8101 aaaaatttga gcaatctctt tttgagttgc atataacctc ctaatctttt ccgggttttt
     8161 aaggcaactg atcttgaaat tagattagca ttaaaaagtc ctaaaatcaa acaaaagttc
     8221 gaatcccacc atgggtatca aaaatcaggc tttatacaag cctgaaaata acgtagctcc
     8281 ccctgcagga caaacttaga actttggatt ggagtgttat caaacgagaa ttggttattt
     8341 tacaacacta tcttgcaatt taaaaaatag cccgaagaac ttcacagtcc tccgggcttt
     8401 tgtttgccgc aattgcggtc ttacttgatg gccaggaaat cctggcagtg ggcttgttct
     8461 ccctccc
//
LOCUS       DATKGS010000070         3284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_177710, whole genome shotgun sequence.
ACCESSION   DATKGS010000070 DATKGS010000000
VERSION     DATKGS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177710"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VMQ48_01705"
     CDS             <1..366
                     /locus_tag="VMQ48_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HUD08788.1"
                     /translation="KHMIDPQIQEELKQKLLEEKKRIEENLSKTETGKKDADREYETA
                     FPEVDRDEESNADEMDMYENNLAADEAMKGELEKIEKALAAMDAGTYGFCANCQKDIP
                     LERLHAYPQADTCLDCEEK"
     gene            383..781
                     /locus_tag="VMQ48_01710"
     CDS             383..781
                     /locus_tag="VMQ48_01710"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUD08789.1"
                     /translation="MKTNTKLAFLFLVFIAVDQLAKNVFAFTSFCNKNIAWNIPVHAG
                     IFYFVWIAIVASLIYIFFKSKNIYPKIFLIIILSGAISNIIDRIRLGCVVDYIDLKFW
                     PVFNLADVYITVGIILLLILNLKFEILSES"
     gene            873..2399
                     /locus_tag="VMQ48_01715"
     CDS             873..2399
                     /locus_tag="VMQ48_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HUD08790.1"
                     /translation="MSSKVLSATTLGLTSHLVEVEIDTHSGMYVFSIVGLPDATVKEA
                     KDRVNAAIKNCGFKPPYQCGHVTANLAPADIQKVGPLYDLPIALGFLLTTKQIDFDAS
                     DKLFVGELSLDGKIRPVNGVLPIAMMAKEKNISEIFVPKENAGEASIAGGLKVIPADN
                     FKDLVAHLLGEKIIDPHPEQDLQKVFEPQTYHLDMAHVKGQEHAKRALEIAAAGGHNV
                     LFSGPPGSGKTLLAKTFPSILPPLTFPEALEITKIFSIAGKLPREVALVSQRPFRSPH
                     HSASAVSLVGGGQFPRPGEISLSHRGVLFLDEFPEFSRMVLENLRQPLEDGSISVSRA
                     QATLSFPARFTLIAAMNPCPCGNATDPEKLCSCSPSSIIRYQRKISGPILDRIDIHIE
                     VPRMKFDKLASEKLGESSESIRKRVTAARQRQQARFKNLDGIIANSEMTGQQMKEFCQ
                     LGNNELDLLRKAVNQLQLSARAYHRIIKIARTISDLAGAEQIEIPHLAEAIQYRQRQT
                     "
     gene            2460..>3284
                     /locus_tag="VMQ48_01720"
     CDS             2460..>3284
                     /locus_tag="VMQ48_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HUD08791.1"
                     /translation="MKKKISILLLFFAIAAVLYFFWPFGKRKFNVKLPFPVAVADSGN
                     YFAATTSARTISDFLKEKKIALGDKDFIFPAPDAKIFPGSQIILRHAVPVVIAADGKN
                     IQLATTGRTVSDALSEANVSLSPVDKIAPDPSQTLTANLKIVVTRINIAEIAVDEDIP
                     FQVVQKEDNTVLWKQNKITQKGVNGIREKKYRVTYTDGKETNRVLIGTAVTQAPISEI
                     DSIGTKIIVGKTQSGDATWYTNGDDLTCASLTFSHGEYLRVTNRSNGKSVIVQVNDS"
BASE COUNT          936 a          813 c          719 g          816 t
ORIGIN      
        1 aaacatatga tcgatccaca aatccaggag gaactcaaac aaaaactgct tgaagaaaaa
       61 aaacgaatcg aagaaaacct gtccaaaaca gaaaccggaa agaaagacgc tgatcgggaa
      121 tatgagaccg cctttccgga agtcgaccgc gatgaagaat ccaacgccga tgaaatggat
      181 atgtacgaaa acaaccttgc cgccgatgaa gcgatgaagg gagagcttga aaaaatcgaa
      241 aaggctctcg ccgcgatgga tgccggcacc tatggatttt gcgccaattg ccaaaaagac
      301 atcccgctcg aacggctcca cgcctatccg caggcagaca cttgtcttga ttgcgaggag
      361 aaataaaaca tacaaaagga ggatgaaaac aaacacaaaa ctagcatttc tttttcttgt
      421 ttttattgct gtcgatcaac ttgcgaagaa tgttttcgct ttcacttctt tttgcaacaa
      481 aaatatcgct tggaatattc cggttcacgc cggaatattt tattttgtct ggattgcgat
      541 cgtcgccagt ttgatctata tattttttaa atccaaaaat atttatccga aaatatttct
      601 aatcattatt ttatctggcg caatctcaaa cataatcgac cgaatccgcc tcggctgcgt
      661 cgtcgattat atcgacctca aattttggcc agtatttaat ttagcagatg tgtatattac
      721 ggttggaatt attttgttat taattttaaa tttgaaattt gaaattttaa gtgaatccta
      781 aatgattaaa ttttaaatgt tttcaaattt tgtcatttag ttattcaaaa ttgatttaaa
      841 atttataatt tagaatttaa aattttcagt ctatgtcttc aaaagtactc agcgcgacaa
      901 ctttaggatt gaccagccat ctggttgaag tggaaatcga cactcattcc ggaatgtacg
      961 ttttttcgat cgtgggtctg ccggacgcga cagttaaaga agccaaagac cgcgtgaacg
     1021 cggccatcaa aaattgcggc ttcaagccgc cgtaccagtg cggtcatgtc acagccaatc
     1081 tcgctccggc cgatatccag aaagttgggc cactctatga tctgccgatc gcgctcggat
     1141 ttttgctcac caccaagcaa atcgattttg atgcgtcaga caaattattt gtcggagaac
     1201 tttctctcga cggaaaaatt cgcccagtga acggagtttt gccgattgcc atgatggcga
     1261 aagaaaaaaa tatttccgaa atttttgtgc caaaagaaaa cgccggagaa gccagtatcg
     1321 cgggcggcct caaagtcatt cctgcggaca atttcaaaga tttggtcgcg catcttcttg
     1381 gggaaaaaat aatcgatccc catcccgaac aagatcttca aaaagttttt gagccgcaga
     1441 cctatcatct tgatatggct catgtcaaag gccaggaaca cgccaagcgc gcgcttgaaa
     1501 tcgccgcggc cggcggacac aatgttttgt tttccggacc gccaggatca ggaaaaacgc
     1561 ttctcgcgaa aacatttccc tcgattcttc cgccgcttac tttcccggaa gcactcgaga
     1621 tcaccaaaat attttcgatc gccggaaaac tgccccgtga agtcgcgctt gtttcccagc
     1681 gccctttccg ttcacctcat cacagcgcct cggccgtttc tttggtcggc ggaggacaat
     1741 ttccgcgccc gggagaaatc agtttgtccc accgaggagt cctgttcctt gacgaatttc
     1801 cggaattttc ccggatggtc ctcgaaaatt tgcgccagcc gctcgaagac ggttcgattt
     1861 ccgtttcacg ggctcaggcc acgctttctt ttcccgcccg cttcactttg atcgctgcca
     1921 tgaatccttg tccctgcggc aacgcgacgg atccggaaaa attgtgttcc tgcagtccgt
     1981 cttcaatcat cagatatcag cgaaaaatat ccggtcccat tcttgaccgc atcgacatcc
     2041 acattgaagt tccgcgaatg aagttcgaca aattggccag tgaaaaattg ggagaatcaa
     2101 gcgaatcaat tcgaaaaaga gtgactgccg cgcggcaaag gcaacaggcc agattcaaaa
     2161 atttggacgg catcatcgcc aactccgaaa tgaccggcca gcagatgaaa gagttttgcc
     2221 aactcgggaa caatgaattg gatctattgc gaaaagccgt caatcagctt cagctttccg
     2281 cccgcgccta tcaccgcatc atcaaaatcg cccgcactat cagtgacctc gccggcgcgg
     2341 aacaaatcga aatcccacac ctcgccgaag cgatccagta caggcagagg cagacgtgaa
     2401 aagctttttc ttttcttcaa aatagtgtat attatacagc tagctggggc tgttttctta
     2461 tgaagaaaaa aatctcaata ttgctcctgt ttttcgcgat tgccgctgtt ttgtattttt
     2521 tttggccgtt tggaaaaagg aaattcaatg tgaagctgcc ttttccggtt gcggtggcgg
     2581 atagcggcaa ctattttgca gccacaactt ccgcgcgaac catttctgat tttctcaaag
     2641 aaaaaaagat tgcgctcggc gataaggact ttattttccc cgcgccggac gcaaaaattt
     2701 ttccgggcag ccaaatcatc ctccggcacg cggttccggt tgttatcgcc gccgatggga
     2761 aaaatatcca gctcgccacg accggccgaa cggtcagcga tgcgctctcc gaagcaaatg
     2821 tttcgcttag cccggtcgac aagatcgctc cagatccgtc ccaaaccctg accgccaatt
     2881 tgaaaattgt cgtgacaaga atcaacatcg ccgaaatcgc ggtagacgaa gatattcctt
     2941 tccaggtggt gcaaaaagaa gacaacacgg ttttgtggaa acaaaacaaa atcactcaaa
     3001 agggtgtgaa cggcatccga gagaaaaaat accgcgtcac ttatacggac ggaaaagaaa
     3061 ccaatcgtgt tttgatcgga accgccgtca cccaggcgcc aatatcggaa attgattcca
     3121 tcggaacaaa gatcattgtc ggaaaaaccc agagcgggga cgcgacctgg tacacgaacg
     3181 gggacgacct cacctgcgcc agtctgacct tctcgcatgg agaatacctg cgcgtgacga
     3241 accgctccaa cggaaaatcg gtgatcgtcc aagtgaacga ctcc
//
LOCUS       DATKGS010000071         8227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_7478, whole genome shotgun sequence.
ACCESSION   DATKGS010000071 DATKGS010000000
VERSION     DATKGS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7478"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..791)
                     /gene="lexA"
                     /locus_tag="VMQ48_01725"
     CDS             complement(150..791)
                     /gene="lexA"
                     /locus_tag="VMQ48_01725"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HUD08792.1"
                     /translation="MQLKITEKQKNALGVIYESIRLSGFPPTFADLREKLNVASNQAV
                     LNFLESLEKKGFIKREDGQARSIKILPLGYKEIEKEPLIKIAGMSAAGSCIESFADAF
                     TAWMPLSDKIFQNEKISKAQDDVFVIQVHGDSMINAGIDDGDMLLIKKSKEYKSGDIV
                     VARSDEGTTVKRFVADGGKRYLKPENPNYENIPIIPGEINFEGKVILNLSKVR"
     gene            1074..3359
                     /locus_tag="VMQ48_01730"
     CDS             1074..3359
                     /locus_tag="VMQ48_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HUD08793.1"
                     /translation="MGRRKKKDKEKNGKNGNGNGNGYKPKKKWGLAGDTKRSIAAVFL
                     IALAILAVFSLFGGAGTFGTYFNKILSLAFGAGRFLLPVLMIVVGFMYFRRDTDSYYV
                     FVAGGFFVFFAAILGFIHIFYPLSKMVAMAQSGQGGGFLGAAAAYPALRLFGFSAGII
                     VLLALALASLLLALNVPLRALLKPVEWMKKRSDEEEEGETSISKPPAAAEALVGKSTA
                     VRISEIIKGRTEKSAPDRKMMMPDKQRMIAGWKLPPLDLLERTTGKSKAGDIEANAKI
                     IQETFKNFGIETEIGEISVGPSVTQYCFKPAAGIKLSKITGLNNNLSLALAQHPIRIE
                     APIPGKSLIGIEVPNKVSTMVRLRDFVESVKFAERKTNLTLALGQDVSGNYIFGDLTE
                     MPHLMIAGATGTGKSVSINTIITTLLYQNSPDDLKMILIDPKRVELTAFNGIPHLLTP
                     TVVENGKVANSLKWAIGEMERRYKMLQESASRDIFSYNEKIKPEQKKNIINEETGEVL
                     GEEEVKRLPYIVIIIDELADIMASHSKDVEAAIVRLAQMARAVGIHLIVSTQRPSVEV
                     LTGLIKANITTRIAFQVATQIDSRTILDMGGAEKLLGNGDMLYLSARSPKPKRIQGVF
                     VSESEVKRVVKSIKSQLDGEVEYDEEVTATQRVSFSGKMTGAFGGDQEGDELYEEAKD
                     TVIKSGKASASLLQRRLRVGYARAARLLDILEANGVIGQADGSKPREILVDSPVSPVA
                     TGEVQYKDELDDQKKRERWQM"
     gene            3452..4453
                     /locus_tag="VMQ48_01735"
     CDS             3452..4453
                     /locus_tag="VMQ48_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HUD08794.1"
                     /translation="MFRGFTQKKIHSYTLGEQLKNLRSEGRVTLFEVSRETKIPVKYI
                     EMIEEGKYEKLPPDVYVKGFLRAYAEFLGIEPKKIIKLYEREKDIKNNLNGGEKKAPQ
                     VKKKTRLSHFVVTPRIIIAVSVAVVVIGGFSYLYSQIGRFAAVPRLVITSPASDENIP
                     GNSISVSGYTDEDAKLTINGQPVLVSDKGEFKEDILLQSGVNSITISALNRFNKEISK
                     TVNIKSDYQSPSLAADNSSGDQGKVSGEQDVKKSGVDVVVRADSIPIWISVEADGNLV
                     YSGTMLAGAVQNFDGEKEVRVTSGKANQTFIKVNGKAEKILADSPGIVRDVVFTPSD"
     gene            4473..5465
                     /gene="recA"
                     /locus_tag="VMQ48_01740"
     CDS             4473..5465
                     /gene="recA"
                     /locus_tag="VMQ48_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUD08795.1"
                     /translation="MSKETENKEAILENTVKEIKDRFGEGSIMKLGDVKRVDVAAIPT
                     GAPSLDIALGIGGVPRGRIIEIYGPESSGKTTLALHIVANAQKTGAAAFIDAEHALDP
                     SYAKKIGVNLEDLLISQPDNGEQALDIVEALVKSNAVDVIVIDSVAALTPQAEIEGEM
                     GDSHMGRQARLMSQALRKLTAIGAKSNVTIIFINQIRMKIGVVFGNPETTTGGNALKF
                     YASVRIEVRRAAQIKQGEQIVGNRVKAKIVKNKVAPPFQVTEFDIMYNEGISKEGDLI
                     DTGVKYEVVAKTGNSFSFGEVKLGVGREKAKTFLRENPKVYKEIEKALKEKVKV"
     gene            complement(5533..6093)
                     /gene="efp"
                     /locus_tag="VMQ48_01745"
     CDS             complement(5533..6093)
                     /gene="efp"
                     /locus_tag="VMQ48_01745"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HUD08796.1"
                     /translation="MLNLSDIKTGKTLSINNEPYLVIFHQHSKMGRAGAVLRTKLRNL
                     KNGAVMEKTFQGSDKIEEAEMAKSKAQFLYREGENYFFMDNTSYEQFSLPKSVLGDLT
                     DYLIEGTEVTILVYGTTPINIELPVKMDFKVVEAPPAIKGNTADGGSKQVTLETGIKV
                     NTPLFVKEGDIIRINTETGEYSERAK"
     gene            complement(6097..6336)
                     /gene="rpsR"
                     /locus_tag="VMQ48_01750"
     CDS             complement(6097..6336)
                     /gene="rpsR"
                     /locus_tag="VMQ48_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HUD08797.1"
                     /translation="MKFNAQQNTPPAAPKTCYFCANNVHTIDYKDTQLLRRFMNAHAK
                     IVPPRRTGNCAKHQRMVTRAIKRARTMALLSYTIK"
     gene            complement(6378..6872)
                     /locus_tag="VMQ48_01755"
     CDS             complement(6378..6872)
                     /locus_tag="VMQ48_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HUD08798.1"
                     /translation="MSLNKVLLIGNLTRDPELRSTPSGQSVANFAIATNRVWKDPQSG
                     EKKEAVEFHNIVVWRRLAEIAGQYLRKGSKIFLEGRLQTRSWQDQSGTKKYMTEIIAE
                     NMQMLDRKPQGGNAPYQSAPMPSGPAMPPQSANQPLPPQPEEIPTIQIDENQDEVKIE
                     DIPF"
     gene            complement(6988..7479)
                     /gene="rpsF"
                     /locus_tag="VMQ48_01760"
     CDS             complement(6988..7479)
                     /gene="rpsF"
                     /locus_tag="VMQ48_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HUD08799.1"
                     /translation="MEYELLFFTSVANEDRIGEIKREIDEIITGRGGQKTADFSDIGK
                     RKFAHPIKKQTHGFFSFCRFTIEDAENLPELNKRLALNDKIMRHIIVRADEIGKPIGA
                     PAMPDAKQDARPERPSIKRTEPARVAPQSTASEKKPAEETAKVEMAELDEKLNAILDE
                     SPE"
     gene            7721..7891
                     /locus_tag="VMQ48_01765"
     CDS             7721..7891
                     /locus_tag="VMQ48_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08800.1"
                     /translation="MLDEDKIKKDLEKMREAYAIASKKLDDIRERQAKIAEKIYAARK
                     DNSAKNNKILWA"
     gene            7882..8178
                     /locus_tag="VMQ48_01770"
     CDS             7882..8178
                     /locus_tag="VMQ48_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08801.1"
                     /translation="MGLETFWGKKIETASPENYKVELEKEEKEVMEKVTAIFQENAEL
                     NKLLLDKEDLDFSEHQTRYLAGQLTGFRRDTKDVIENLRNIVPVQTADRTKKAA"
BASE COUNT         2195 a         1768 c         2121 g         2143 t
ORIGIN      
        1 ggggggaata ttttccgtct ttttccgaaa aatcaagcac ctcgtcaaaa tttattccct
       61 caaacacaga aagtccgtat tgcatgtccg gatttctgaa tattttttcc agaacaatgc
      121 cagataaatt tttttcagtc atattttttt tatcttactt tacttaaatt taaaataact
      181 ttgccttcga aatttatttc ccccggaatg atagggatat tttcataatt gggattttcc
      241 ggcttcaaat atctttttcc gccgtcagcg acgaatcttt tcacagtcgt gccttcatcg
      301 cttctcgcga cgacgatatc accggacttg tattctttgg attttttaat cagcagcata
      361 tcgccatcgt ctatgccggc gtttatcatt gaatctccat gaacttgaat gacgaaaaca
      421 tcatcttgcg ctttgcttat tttttcgttt tgaaaaattt tatcagatag cggcatccaa
      481 gcggtaaacg cgtcagcaaa cgattcaatg caggatccgg ccgcgctcat tccggcaatt
      541 tttatcaatg gttctttctc aatttctttg tatccaagag gcaaaatttt tatacttctg
      601 gcttgtccgt cttctctttt aataaatccc tttttttcaa gagattccag aaaattcaaa
      661 acggcctgat ttgaagcaac attgagcttc tcgcgaagat cagcaaacgt cggaggaaag
      721 cccgataatc tgatactttc atagatcaca ccaagtgcat ttttttgttt ttctgttatt
      781 tttagttgca tatccctatt atatatgacc atatagtcat gtcaagtcaa gataatatgg
      841 cttatttaag ctatattaaa aatttatcca cagtttggtc acgcaaaaaa attctttata
      901 ttttcagaaa ccggagagat gttcgctaag ggtcggttaa tccaaggttc gacctttccc
      961 ttgtcggctg tcgcctccaa ttcctcaagg tcgaaccttt ctagctaaac tggaaatctc
     1021 tcggggaata tgctagaata agcaagtaat aatctttacg aaaatagaaa acaatgggcc
     1081 ggagaaagaa aaaagacaaa gaaaaaaacg ggaaaaacgg gaatgggaat ggaaatgggt
     1141 acaaacccaa gaagaagtgg ggtttggccg gggatacaaa gcgcagcatc gcggcggttt
     1201 ttttgatcgc gctggcgatt ttggcggtgt tcagcttgtt cggcggggcg gggacgtttg
     1261 gcacgtattt caataaaatc ctctcgctcg cttttggagc gggtcgtttt cttttgccgg
     1321 ttttgatgat cgtggtcggc ttcatgtatt tccggcggga tacggattca tattatgtct
     1381 ttgtggcggg cggttttttt gtattttttg cggcgattct cgggttcatc catattttct
     1441 atcccctttc gaaaatggtg gcgatggcgc aaagcgggca aggcggggga tttttggggg
     1501 cagcggcagc ctatccggcg ctcaggttgt ttggcttttc ggcggggatc attgttcttc
     1561 tggcgcttgc tctcgcgtcg cttctgcttg cgctaaacgt gccgttgcgg gcgctcttga
     1621 agccggtgga atggatgaaa aagaggagcg acgaggaaga ggaaggagaa acttctattt
     1681 caaaaccgcc cgccgccgct gaagctttgg tgggtaaatc cacagcggtg agaatcagcg
     1741 agattatcaa gggccggacg gaaaaatcgg ctcccgatcg gaaaatgatg atgccggaca
     1801 agcaacggat gatcgcgggt tggaaattgc cgccgcttga tctcttggaa aggacgaccg
     1861 gcaaatccaa agcgggcgat atcgaagcca acgccaaaat catccaggaa actttcaaaa
     1921 atttcgggat cgaaacagag atcggcgaaa tcagtgtcgg accgtccgtc acgcaatatt
     1981 gtttcaaacc ggcggccggg atcaagcttt ccaaaatcac cggactcaac aacaatttgt
     2041 cgctggcgct cgcccagcat ccgatccgga tcgaagcgcc gattccgggg aaatctctca
     2101 tcgggattga agtcccgaac aaagtttcaa ccatggttcg tttgcgcgat ttcgtggaga
     2161 gcgtcaaatt tgccgagcgg aaaacaaacc tcactctggc gctcgggcag gatgtctccg
     2221 gaaactatat tttcggcgat ctcacggaaa tgccgcatct catgatcgcg ggagcgaccg
     2281 ggacggggaa gagcgtttca atcaatacga tcattactac gcttctctat caaaactcgc
     2341 cggacgatct gaaaatgatt ctgattgatc caaagcgagt ggaactgacg gctttcaacg
     2401 gcataccgca ccttctcacg ccgacggtgg tcgaaaatgg aaaagtcgcc aattctctca
     2461 agtgggcgat cggtgaaatg gaacggcgct acaaaatgct ccaagaaagc gcatcacgcg
     2521 atattttttc gtataatgaa aagataaagc cggagcagaa gaaaaatatt atcaacgaag
     2581 agacggggga agtgctcggc gaggaggaag tgaagcgcct gccgtatatt gtgatcatca
     2641 ttgatgagct ggcggacatc atggccagcc attccaaaga cgtggaagcg gcgatcgtgc
     2701 ggttggcgca aatggcgcgc gcagtcggca tccatctcat cgtttcgacc cagaggccgt
     2761 cagttgaagt gttgaccggg ctcatcaagg ccaacatcac gacgcgcatc gcctttcaag
     2821 tggcgacgca gatcgattca agaacgatcc ttgacatggg cggggcggaa aagttgcttg
     2881 gaaacggaga tatgctctat ctttccgccc gttcaccgaa accgaaacgg atccagggag
     2941 ttttcgtttc cgaatcagaa gtgaagcgag tcgtgaaatc aattaaatcc caacttgatg
     3001 gagaagtgga atatgacgag gaagtcaccg caacgcaaag agtcagtttt agcggaaaga
     3061 tgaccggagc gttcggcggc gatcaggaag gggatgagct ctatgaagaa gccaaagaca
     3121 cagtgatcaa atccggcaaa gcatccgcgt ctcttttgca gcggcggctc cgggtgggct
     3181 atgcccgcgc ggcgcggctt ctcgacattt tggaggccaa cggcgtgatc ggacaggcgg
     3241 acggatcaaa acctcgggaa atcttggtag atagccctgt ttctccggtc gcgaccggag
     3301 aagttcagta taaagatgag ctggatgacc aaaaaaagcg ggaacgttgg caaatgtgag
     3361 cggatgagtt ataatgatgg agaaaataaa aacaaaaatc tggcaggttg tttgacctgt
     3421 ttttattgtg gatttaacaa ttctcaaata aatgtttcgc ggtttcacgc aaaaaaagat
     3481 ccattcctat accttgggag agcagctcaa aaaccttcgc agcgaagggc gggtgacgct
     3541 ttttgaggtt tcgcgagaaa caaaaattcc ggtgaaatac attgaaatga tcgaggaagg
     3601 gaaatatgaa aagcttcctc cggacgtcta tgtgaaaggt tttctccggg cctatgccga
     3661 atttcttggg atagaaccaa aaaaaataat caaattgtat gagcgggaaa aggacatcaa
     3721 gaacaatctg aatggcggag agaaaaaagc cccgcaggtg aaaaagaaaa caagattatc
     3781 tcacttcgtc gtcacgcccc gcatcatcat tgcagtttcg gtcgcggtcg tggtgatcgg
     3841 cgggttttcc tatctctata gccagatcgg ccggttcgct gcggtgcccc gcctggtgat
     3901 cacgagtccc gcgagcgatg agaacattcc cggcaattcc atttccgttt cgggatatac
     3961 cgacgaggac gccaagctca ccatcaacgg ccagccggtc ttggtgagcg acaagggcga
     4021 gttcaaagag gatattcttc tccagagcgg cgtcaattca atcaccattt ctgccctgaa
     4081 ccgcttcaac aaggaaattt ccaagacggt caatataaag tcggactatc agtcgccaag
     4141 tctggcggcg gataacagca gtggagatca aggaaaagtg agcggagagc aagacgtgaa
     4201 aaaaagcgga gttgatgtcg ttgtccgggc tgattccatc ccgatctgga tttccgtcga
     4261 ggcagacgga aatttggtct attccggaac gatgctcgcg ggagcagtcc agaatttcga
     4321 cggggaaaaa gaagtgcggg tgacgagcgg aaaagcgaat caaacgttca tcaaggtgaa
     4381 tgggaaagcg gaaaaaattt tggccgacag cccgggaatt gtgagagacg tcgtgtttac
     4441 acccagtgat taatatttta attataagga tcatgtccaa ggaaacagaa aacaaagaag
     4501 ctattttgga aaacaccgtg aaagaaatca aagatcgatt cggcgaagga tcaatcatga
     4561 agctcggcga cgtgaagcgg gtggacgtgg cggcgattcc gaccggcgcg ccttcgctcg
     4621 acattgcgct aggcatcggg ggcgtgccac gaggaagaat cattgaaata tacgggccgg
     4681 aatcttccgg gaagacgacg ctcgcgctcc atattgttgc caatgcccaa aagaccgggg
     4741 cggccgcttt catcgacgcg gagcacgcgc ttgatccgtc gtatgccaag aaaatcgggg
     4801 tgaatttgga agatcttttg atctcgcagc cggacaatgg ggagcaggcg ctggatatcg
     4861 ttgaagcttt ggtgaaatcg aatgccgtgg atgtgattgt gattgattcc gtggccgctc
     4921 tcacgcccca agccgagatc gaaggtgaaa tgggtgattc gcatatggga cggcaagccc
     4981 ggctgatgtc gcaggctctc cgcaagctca cggcgatcgg cgccaagagc aacgtgacga
     5041 ttatcttcat caaccagatc cgaatgaaaa tcggagtggt ttttggcaat cccgaaacaa
     5101 ctacgggcgg aaacgccttg aaattctatg cctccgtccg catcgaagtc cgccgcgcgg
     5161 cgcagatcaa gcagggggag cagatcgtcg gtaatcgcgt gaaagcaaaa attgtgaaga
     5221 ataaggttgc ccctcccttt caggtgacgg aattcgacat tatgtacaac gaaggtatca
     5281 gcaaggaggg cgatctcatc gacacgggtg tgaaatatga agtggtcgcc aaaaccggaa
     5341 attcattttc cttcggcgaa gtgaagctgg gggtcggccg tgaaaaagcg aaaacttttt
     5401 tgcgagaaaa tccgaaagta tacaaagaaa tcgaaaaagc gctcaaagaa aaagtgaaag
     5461 tataaaattt tttgaaataa aaaagcagct gcttatgcgg ccgctttttg ctttatataa
     5521 aatgttattt tactatttcg cgcgttccga atattctccc gtttcggtgt tgatgcggat
     5581 gatgtcgcct tctttcacaa acagcggcgt gttgactttg atgccggttt cgagggtaac
     5641 ttgctttgat cctccgtcag ccgtgttgcc tttgatggcg ggcggagctt cgacgacttt
     5701 gaaatccatt ttaacgggaa gttcgatatt gatcggagtg gtgccgtaaa ccaggatggt
     5761 cacttctgtg ccttcgatga gatagtctgt gagatcgcct agaacggatt tcggaaggga
     5821 aaattgctca tagctggtat tgtccatgaa aaaatagttt tctccttcgc gatagagaaa
     5881 ttgcgctttc gattttgcca tctccgcttc ttcgatcttg tccgatccct gaaaagtttt
     5941 ttccatcact gcgccgtttt tgagattgcg aagtttggtg cgcaaaactg ctcccgcgcg
     6001 tcccattttg gaatgttgat gaaaaataac gagatagggc tcgttattta ttgatagggt
     6061 ttttccggtt ttgatgtcgg agagattcag catggtttac tttatcgtat atgaaagcaa
     6121 agccattgtt cgcgctcgct tgatggctcg ggtcaccatc cgttggtgct tggcgcaatt
     6181 gccggttcgg cggggcggaa caatcttggc gtgggcgttc atgaagcggc gcaaaagctg
     6241 cgtgtcttta taatcgatgg tgtggacatt gttggcgcag aaatagcagg ttttcggcgc
     6301 ggccggaggc gtgttttgtt gtgcgttgaa tttcatagcc ataatcgtgt attgtgcaaa
     6361 aatgcggttt atgttggtta aaatgggata tcttcaattt tcacttcatc ctggttttcg
     6421 tcgatctgga tggttgggat ttcttccggc tggggaggaa gcggctggtt ggcgctctgc
     6481 ggcggcatgg ctggtccgga cggcatgggg gcgctttggt atggcgcatt gccaccttgc
     6541 ggttttcggt cgagcatttg catattttcc gcgatgatct cggtcatata ctttttggtt
     6601 ccgctttgat cttgccagga gcgggtctgc aggcggcctt cgaggaaaat cttgctgcct
     6661 tttcgcaaat attgtccggc gatctccgcg aggcgtcgcc acacgacgat gttgtgaaat
     6721 tcaacggctt cttttttctc tccggactgc gggtctttcc aaacccggtt cgtcgcgatc
     6781 gcgaaattgg caactgattg tccggaggga gtggaacgca attccggatc gcgggtgaga
     6841 ttgccgatga ggaggacttt gttgagactc ataatattaa attaaaaatt taaaaatcaa
     6901 agtggaaaat tacagttcaa aattcaaaat gggaaaatgt caaaaatatt tttgaattca
     6961 atataatttt acattttaaa tctttgttta ttccgggctc tcgtccaaaa ttgcattcaa
     7021 cttttcatcc aactccgcca tttcaacctt ggctgtttct tcggcgggct ttttctcgct
     7081 ggcagtgctt tgcggggcga cgcgggcagg ttcagttcgt ttgatcgacg gccgttcggg
     7141 tcttgcgtct tgttttgcat ccggcatcgc cggggcgcca atcggctttc cgatttcatc
     7201 ggcacggaca atgatgtggc gcatgatttt atcattgagc gccagtcttt tgttcaattc
     7261 cggaaggttt tccgcgtctt cgatcgtgaa tcggcagaaa ctgaaaaaac cgtgcgtttg
     7321 ttttttgatc gggtgggcaa attttctttt tccgatgtcc gaaaaatcag ccgttttttg
     7381 tccaccgcgg ccggtgatga tttcgtcaat ttctcgcttt atctccccaa ttcggtcttc
     7441 attcgcaacc gaggtgaaga acagaagttc gtattccatg atgttagttt aaaattaaaa
     7501 aatcaaaatg taaaatgaca atgcaaagtg caaaattatt gattattgtg cttgttcagg
     7561 ctaataacaa aaatcaccat acatgaaagc ccaatcatct ggtgatatct cgcttcttat
     7621 ccgcaaggcg agaagcgtcg ggacttccta agttgtataa atagcctatc atattgactt
     7681 atgtttggca atactagtaa aaatatgcta aaatagagct atgttggacg aagataaaat
     7741 aaaaaaggat ctcgagaaga tgcgtgaggc gtacgcgata gcctctaaaa agctcgatga
     7801 tatccgggaa agacaggcca aaatcgccga aaagatttat gctgccagaa aagataattc
     7861 agctaaaaat aacaaaatat tatgggccta gaaacttttt ggggaaaaaa aatagaaact
     7921 gcttcacccg aaaattataa agttgagctc gagaaagaag aaaaggaagt gatggaaaaa
     7981 gtcacggcga tttttcagga gaacgctgaa ttaaacaagc tgctcctcga taaagaagat
     8041 ctagatttta gtgagcacca gacacgttat cttgccggac agttgaccgg atttcgccgg
     8101 gacacaaagg atgtcattga aaatttaaga aacattgtgc cagtgcaaac ggcagataga
     8161 acaaaaaaag cggcttagat cttgaattcg accacgtctc cgtcttggac gatatattct
     8221 ttccctt
//
LOCUS       DATKGS010000072         4847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_94131, whole genome shotgun sequence.
ACCESSION   DATKGS010000072 DATKGS010000000
VERSION     DATKGS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94131"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VMQ48_01775"
     CDS             <1..330
                     /locus_tag="VMQ48_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HUD08802.1"
                     /translation="PYSSRAAFEVWVMPKDHKPYFERINNEEKIDLGEALQKSIGSIY
                     KTLEDPAYNFYIHTSPCDGKDYPHYHWHIEILPHTATWAGFELSTGIEISTIEPERAA
                     EELKLKI"
     gene            457..939
                     /locus_tag="VMQ48_01780"
     CDS             457..939
                     /locus_tag="VMQ48_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HUD08803.1"
                     /translation="MFHIDYSHIFANIPPQLATFLIAMVPISELRAAIPIGIKVYHLS
                     IWSAYFWSVLGNLVPMILILLILQPIADFLSRHFRIFHKFFEWLFEHTRKRGAKKFEK
                     WGEFAVFILTAIPIPLLGGWTGPLAAFVFNIKLKKSIPLVILGCMAAGIVVTLITVYF
                     "
     gene            984..1751
                     /gene="tpiA"
                     /locus_tag="VMQ48_01785"
     CDS             984..1751
                     /gene="tpiA"
                     /locus_tag="VMQ48_01785"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HUD08804.1"
                     /translation="MHKKLVVGNLKMNPVSVVEFEHYLDMLEKELKNKEFEKTEVVVC
                     PPIVFLQKLLNRDIKNIKAGVQDVFWEYQGAFTGQISAGMVKSVGASYVIVGHSERRK
                     YFGENEEIINLKLKAVLKNGLNAILCIGESAEERKKGQTSQMLKKQLKECLSEIAPGK
                     LEYVSIAYEPIWAVGTDKIPTSDEILEAKIIIKKALAEMFSPKSAEKIRLLYGGSVKS
                     SCVDQVCLDPAMDGALVGRESLMPYEFVKIAKMIDDN"
     gene            1894..2745
                     /locus_tag="VMQ48_01790"
     CDS             1894..2745
                     /locus_tag="VMQ48_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HUD08805.1"
                     /translation="MLASVKDILQEAKNNKYAVGAFNTSNMEVTQAIIRAAIKKNTPI
                     IIQATAKTLDYAGDKVIIELIKAIIEQESASIPIGFHLDHGRDFDTCVRAVELGMKSV
                     MIDASRNELRENMAITKRVVEYAHPKDVMVQAELGKVAYLAEMESNPDWDKLMTNPLE
                     AKWLIEETGADALAVSIGNAHGFARERPEPDWERLGEINKLLPDTFLILHGASDWVNH
                     RVTEAVKGGIVCFNIDTDIKIAFNRVVCKLTSDRCSFVDPRELLGEAREAVQRVVEEK
                     IDLFRNA"
     gene            2772..4349
                     /locus_tag="VMQ48_01795"
     CDS             2772..4349
                     /locus_tag="VMQ48_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUD08806.1"
                     /translation="MPKQINPQLVRRLKHFSEILGIIVFLVGLAALVGWQFDVVFFKR
                     ILPSLPIIAPNTAFSFFLGGIVLLLIANPKTEEKRHLKAVAYIFSSFIALVGFLTLLE
                     YLFGANFGIDRVLFAAAQGETAVRMSPQSAFNFLMMGVSLLFIAGQEKEKVKVGQAII
                     LLAGAVSLVSLFGFIYNISSFYTISSFKGMAAHTAISFTMVFFSVLLIFPDFGFMRIF
                     VSRGLSGLAARRLLFVLVCMIAAEVLATIGRRINIFDYSFEMLIHLVVILGAFVYLIF
                     FAFRSLDELGKMETSLAKSQELDRAKTEFVGLASHQLRTPLTAISWYTEMLLKKDFGG
                     LNAKQEDYLGEIYAGNQRMIDLVDDLLNTSRIDMGTLKIKPEPVNLVEVMENTLNEFS
                     PQIKRKKISIEKKFSRNLPRVLLDPEHLGIVFQNILSNSVKYTPPKGKIMVEMRRQNS
                     HILVKIADNGWGIPAAQQKRIFTKLFRADNVRKRDTEGTGLGLYIARAIIKKSGGKIW
                     FESKEGKGTTFFITLKV"
     gene            4419..4805
                     /locus_tag="VMQ48_01800"
     CDS             4419..4805
                     /locus_tag="VMQ48_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUD08807.1"
                     /translation="MSNGVKKTILIVEDELPMLKALSDKFTLEGFDILEAKDGAEGLE
                     AAISKKPDLIILDIFMPVMDGKAMFEKLREDAWGKTVPVIVLTNLNPDDKTLDQLMKN
                     GPSYYFVKSKWKLEELVEKVKKELGA"
BASE COUNT         1414 a          974 c         1142 g         1317 t
ORIGIN      
        1 ccgtattcat cccgcgccgc gtttgaggtc tgggtgatgc ccaaagacca caagccgtat
       61 ttcgaacgga tcaacaacga agagaaaatc gatttgggcg aggcgctgca aaaatcgatc
      121 ggatcgatct ataaaactct cgaagacccg gcttacaatt tctatatcca tacctcgcct
      181 tgcgatggaa aagactaccc gcactatcat tggcatatcg agattttgcc tcacacagcg
      241 acctgggcgg gattcgaact ttcaaccgga attgaaattt ccacgatcga gccggagaga
      301 gcggcggaag agttaaaatt gaaaatttaa aaatcaaaaa taaaaatgac agtttaaaat
      361 gaaaaattat ttgaaagcca aaaaaatttt tgcatttgcc gatttctaac tttacattgt
      421 cattttacat tttgatattt ccattttaaa ttatctatgt ttcacattga ctattcccat
      481 atatttgcca atattcctcc ccagcttgcg acgtttctca tcgcaatggt tccgatcagt
      541 gagcttcggg cggcgatccc aattggaatc aaagtatatc atctctcgat ttggtcggcc
      601 tatttctggt cggttttggg aaatttggtg ccgatgattt tgattttgct gatcttgcag
      661 ccgattgccg attttttgtc tcgccatttt cgaatattcc acaaattttt cgaatggctt
      721 tttgagcaca cccggaaaag gggagcgaaa aaatttgaaa aatggggaga atttgccgtt
      781 tttattttga cagcgattcc cattccactc ctcggcggct ggacgggacc gctcgcggct
      841 tttgttttca atatcaaatt gaaaaaatca attccgcttg tgattctggg atgtatggcg
      901 gcgggaattg ttgtaacttt aatcactgtg tatttttaag gttgaacctt aaaaatgaaa
      961 aaatggcttt agtttcgtaa attatgcaca aaaaactggt tgttggaaat ttgaagatga
     1021 atccggtgag cgtcgtggaa ttcgaacatt atttggatat gctcgaaaaa gagcttaaga
     1081 acaaagaatt tgagaaaacg gaagttgtgg tctgtccgcc gatcgtattt cttcaaaaac
     1141 tgttgaatag ggatataaaa aacatcaaag ccggggtgca ggatgttttt tgggaatacc
     1201 aaggagcctt caccggacaa atttcggctg gaatggtgaa aagcgtcggg gcgagctatg
     1261 tgattgtcgg acacagcgag agacgaaaat atttcggcga aaacgaagaa ataatcaatc
     1321 tcaaattgaa agcagttctc aaaaacgggc tcaacgcgat tctctgcatc ggggaaagcg
     1381 cggaagaaag aaaaaaaggc cagacctcgc aaatgctcaa gaagcaattg aaagaatgtc
     1441 tttctgaaat agcgccaggc aagttggaat atgtcagcat tgcctatgag ccgatctggg
     1501 cagtcgggac ggacaaaatt cccacttcag atgaaatttt ggaagcaaaa ataatcatca
     1561 aaaaagcgct tgcggaaatg ttttcaccaa aaagcgcgga aaaaatccgc ttgctctatg
     1621 gcggcagcgt gaaatcaagt tgcgttgatc aggtttgtct cgatccagcc atggacgggg
     1681 cattggtcgg tcgcgaaagt ctgatgccgt acgaattcgt aaagattgcg aagatgatag
     1741 acgataatta aattgaaaat gcaaaaatca aaaatcaaaa tgacaatgca aaatgtaaaa
     1801 ttatatttaa ttcaaaaatc attttgacat ttgcccattt tgaactttac attgtaattt
     1861 ttcacttttc actttaaact tttaatttat cttatgctag ccagcgtcaa agacattctt
     1921 caggaggcca agaacaacaa atatgccgtt ggagctttca acacttcgaa catggaggtg
     1981 acgcaagcga tcatccgggc agccatcaaa aaaaatacgc cgatcattat ccaagcgacc
     2041 gccaagaccc ttgattacgc aggcgacaaa gtcatcattg aactgatcaa ggcgatcatc
     2101 gaacaagaat cggcgagtat cccgatcggc ttccatcttg atcatggcag agattttgat
     2161 acttgcgtgc gagcggtcga attggggatg aaatcggtga tgattgacgc ttctcgcaat
     2221 gaattgcgag aaaatatggc tatcacaaaa agagttgttg agtacgctca tcccaaggac
     2281 gtcatggttc aggctgaact tggaaaggtg gcgtaccttg ccgaaatgga atcaaatccg
     2341 gactgggaca aactgatgac aaaccccttg gaagcgaaat ggctgatcga agaaacggga
     2401 gcagatgctt tggcagtttc aattggaaac gcgcatggtt ttgccagaga gcggccggag
     2461 ccagattggg aaagattggg agaaataaac aagcttcttc cggacacttt tctcatattg
     2521 catggcgcga gtgactgggt gaatcacaga gtgacggaag cggtgaaagg tggaattgtt
     2581 tgcttcaata ttgatacgga tatcaagatt gctttcaata gggttgtttg caaattgaca
     2641 agtgatcgat gcagctttgt cgatccgaga gagcttttgg gagaagcaag agaagcagtg
     2701 cagcgagtcg ttgaagaaaa aattgacttg tttcgcaacg catagccctg agttttgcaa
     2761 aaaaacaaaa aatgccaaaa caaataaacc cgcaactggt tcggcggctg aagcattttt
     2821 ccgaaatcct gggaataatc gtttttttgg tcggcctggc ggcgctggtc ggctggcagt
     2881 tcgatgtggt ttttttcaag agaatccttc ccagcctgcc gatcatcgcg ccaaatacgg
     2941 ctttttcttt ttttcttggg ggaattgtct tgctcttgat cgccaatccg aaaacagaag
     3001 aaaaaaggca tttgaaggcc gtagcctata ttttttcctc ttttatcgct cttgtcggat
     3061 ttttgaccct tctcgaatat cttttcggag ccaattttgg gatcgaccgc gtgctttttg
     3121 ccgcggcgca aggtgaaacc gcggtcagga tgtctcctca aagcgctttc aattttttga
     3181 tgatgggcgt ctcactactt tttatcgccg gacaagagaa agaaaaagtg aaagtgggcc
     3241 aagcgatcat ccttctggct ggagctgtct ctttggtctc tttgtttggg tttatctaca
     3301 atatctccag tttctatacg atttcttctt tcaagggtat ggcggcgcat acagccatat
     3361 cattcacgat ggtttttttc agtgtgctgc tgatttttcc ggacttcggt ttcatgcgta
     3421 tattcgtgag ccgcggcctt tccggacttg ccgccagacg attgcttttt gttcttgtct
     3481 gcatgatcgc tgcggaggtt ttggcaacaa tcggaagacg aataaatatt tttgactatt
     3541 ctttcgaaat gctgatccat ctggtcgtga tcttgggagc ctttgtctat ctcatttttt
     3601 tcgcttttcg ctctcttgat gagctaggga aaatggaaac aagtcttgcc aagagccaag
     3661 agcttgatcg ggcaaaaacg gaatttgtgg gtttggcttc gcatcagttg cggacgcctc
     3721 tcactgccat ttcctggtat acggaaatgc ttcttaaaaa agattttgga ggattgaatg
     3781 cgaaacagga agattatctc ggcgaaatat atgccgggaa tcagcggatg atcgatctcg
     3841 tggatgatct tctcaacacc tcgcggatcg atatgggaac gctcaaaatc aagccggaac
     3901 ctgtcaattt ggtggaagtg atggaaaaca cgctgaatga attttcaccc cagataaaaa
     3961 gaaaaaaaat cagcatcgaa aaaaagttca gccgcaattt gccgcgcgtg ttgcttgatc
     4021 cggaacattt gggaattgtc tttcaaaata ttctttccaa ctcggtgaaa tatacgccgc
     4081 cgaaggggaa aataatggtc gagatgcgaa gacaaaactc ccatattttg gtcaaaatcg
     4141 cggacaacgg ctggggtatc ccggccgccc agcaaaaaag gatatttaca aaactcttcc
     4201 gggctgataa tgtaagaaag agagatacgg agggaacggg tcttggcctt tatatcgcca
     4261 gggcaatcat caaaaagtca ggcgggaaga tctggtttga atcgaaagaa ggcaaaggaa
     4321 caactttttt tataaccctt aaagtataaa aattcacccc gttagacatg gtctcgggat
     4381 tacaaattgc ataaaagttt attattaaaa ttaaaaatat gtctaacggg gtaaaaaaaa
     4441 ccatcctcat cgtcgaagac gagttgccga tgctcaaagc cttgtcggac aaattcaccc
     4501 tggaaggatt tgacatcctg gaagccaaag atggcgcgga aggtcttgaa gccgccatct
     4561 ccaagaaacc ggatctcatc atccttgaca tcttcatgcc ggtgatggac gggaaagcaa
     4621 tgtttgaaaa attgagggag gacgcctggg gaaaaactgt gccggtcatc gttctcacca
     4681 atctcaaccc ggacgacaag acgctcgacc agctgatgaa aaacggcccg tcgtactatt
     4741 tcgtcaaatc gaagtggaaa cttgaggaat tggttgaaaa agtcaagaaa gaattgggag
     4801 cctagtttat ttctgtgttt ttaactttcc cgggattttt tcctcgg
//
LOCUS       DATKGS010000073         5540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_209644, whole genome shotgun sequence.
ACCESSION   DATKGS010000073 DATKGS010000000
VERSION     DATKGS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209644"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..852)
                     /locus_tag="VMQ48_01805"
     CDS             complement(226..852)
                     /locus_tag="VMQ48_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HUD08808.1"
                     /translation="MAKTKQIQRLTGRDILFAAINGLIFGALLPVVLKNMNIAMSANK
                     GVIIAIAFTVLAAAGVYIGYLLSKYISVIFQIAKFGCVGAANFAVDIGILNLLIFLSG
                     TAAGIAYIVFKVISFTFAVTNSFIWNKIWTFKKVDNKESGKEFAQFIMISVIGLILNA
                     AVAGFLVIVVGPMGGIKVKTWASVSAAVASLCVMAWNFIGYKFWVFKR"
     gene            complement(877..1599)
                     /locus_tag="VMQ48_01810"
     CDS             complement(877..1599)
                     /locus_tag="VMQ48_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08809.1"
                     /translation="MPKKKTKEIDKDQELQKIAAIREMIAGAERTIASARAMLSQIEG
                     VPMKRAPKIVSSDEEGEIVEGVFDGQVMIGTDGKQYPIPANYASKSKLVEGDMLKLTI
                     TPDGSFVYKQIGPIERKRVIGIVSQDDLGNYVVIAEGKAFRVLLASVTYFKADAGDEV
                     TIVIPRDSETVWAAIENLVKKGAMDGTTFGGFESETQPEQKVAKTMEDNFGKDGRLEV
                     DLVDEWTPDVDKLHAELGKSAI"
     gene            1882..2322
                     /locus_tag="VMQ48_01815"
     CDS             1882..2322
                     /locus_tag="VMQ48_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUD08810.1"
                     /translation="MKKRSATEKIISLMFNMGRAIKKQCLESCGRADGLSILQIEALW
                     HVKEGKEVLMKDLASHLFIAPPSATSLADDLVKAKLVKRSEDKEDRRTTAISLTPKGK
                     RALANFLKKRMEKAKKRIDKLTQAEKKSLFAILEKLAKENDQSR"
     gene            2345..3430
                     /locus_tag="VMQ48_01820"
     CDS             2345..3430
                     /locus_tag="VMQ48_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUD08811.1"
                     /translation="MKNFAKSNRRALTIVAVIALIGVSVWAAFHFTRIPAFSETQIFE
                     VQKGAIKSEISADITLQPATNADLAFENSGKIKNIYVSVGDQVKKGQLLAEEVNSDYV
                     VSLREAEAARRAAQADLEGAQENIDIQGAKLKSLKNANAKKYDVKAQKETKDQAGSSA
                     DSKEALLEAANQAVIAANLQLAKTRLTAPMDGVITDKSIEIGEVVSQAGPVMKIASGD
                     NLEADAYVSELDVKKIAVGGSAEIVLSSADGNKITLDSKIKNIYPAETVQNGVSSYKV
                     VFDLAGQESNLKSGMTGMANIKISDQAGAMVVPQSSVFSDAGKKYVMVMSGGFPERKD
                     VQVGAYGSDGTVEVLSGLAEGDKILKF"
     gene            3435..4100
                     /locus_tag="VMQ48_01825"
     CDS             3435..4100
                     /locus_tag="VMQ48_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HUD08812.1"
                     /translation="MEQEKNNQIADSHSTTKGEKKTRRIMIFALVVFAVAASFIGLVY
                     LDAISKRVYVDKSEVDAPQIMLSAKTGGILEELFVKEGDQISANDVVARVGNELVKSK
                     DAGLVISRQDSIGKYFGPGEEVVTMIKPGDLRIVGRLAEDKGLKDVKVGQRVVFEVDA
                     FGTKQYEGIIDEVSPTSRASDVVFNISDQRQENEFNVKARFNLSQDPELKNGMSAKMW
                     IYK"
     gene            4113..>5540
                     /locus_tag="VMQ48_01830"
     CDS             4113..>5540
                     /locus_tag="VMQ48_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HUD08813.1"
                     /translation="MADNNGQKSTIGRYRWMILITVIVGTFLGRLDQTIVNLALPKII
                     SDYRITVTSAGWIATAYILANAIFVPIWGKLGDTIGRKKVYLWGFAIFIFGSVLAGFA
                     WNLSSLIVFRVIQAIAGSADYPTAMAILAVTFPSGKERAQALGIWSSSFAAAVVFGPL
                     IGGPLIDNFGWRSVFLINFPVGIIGIIMALTYIKESVSEKVTKYFDWWGAITLGVSLA
                     SLVLVLDKGMSWGWTSWESLVSYFVMLVSLAFFIRVENNHPEPIVDFKFFKNNVFTNT
                     LINNFIVFMGMMGSVFLIPIFAQTYLGYDATKTGYLFIPMAFMIILAAPLGGALTGKV
                     QPRYVIAVSTFIAAIGIYFFTGIDARSHAMNIIFPMAVMAFGIGFGMAQRTNIIATVV
                     PQNEIGVASSILALARNIAGAFGIAVFGTILNNSTSSKVLEIARYSSLHAFTPNFVGQ
                     FTSLIILKAQIEAYRTVFLYAFVSLP"
BASE COUNT         1462 a         1216 c         1399 g         1463 t
ORIGIN      
        1 ggggagccgc aaaaaggtaa gcctgcccga cccgccccga tttcacggcg tttttgaggg
       61 tttggacgac gtgcttttgc ccgatcaatt cggaaaaggt ggcggggcga tattttcgat
      121 agagagtggt gctcatactt ggttattata gagaattgag ggggtaaaat caaattttga
      181 aaagaaaaag accgctggaa tgcggccttt aataaaaata agaatttatc ttttgaaaac
      241 ccagaatttg tacccgatga agttccaagc catgacacag agggaagcca cggcggcgga
      301 aacgctggcc caggttttga ccttgatgcc gcccatcggg ccaacgacga tcacaagaaa
      361 acccgcgacg gcggcattca ggatgagacc gatcacgctg atcatgatga attgggcaaa
      421 ttctttgccg gattctttgt tatcaacttt tttgaaagtc caaatcttgt tccaaatgaa
      481 gctgttcgtg acggcaaagg tgaatgaaat tactttgaat acgatatagg cgattccggc
      541 cgctgtcccc gagagaaaga tcaagagatt caggatgccg atgtcaacgg cgaagttcgc
      601 cgcgccaacg catccaaatt tcgcgatctg aaagatcacg ctgatatatt ttgaaagcaa
      661 atagccgata tacactcctg cggcggcgag gacggtgaat gcaatcgcaa tgatcactcc
      721 cttattagcg gacatcgcga tattcatatt cttgagcacg accgggagca gcgcaccgaa
      781 aatcaaaccg ttgatggcgg cgaaaagaat atctcttcca gtcagtcgct ggatttgttt
      841 tgtttttgcc atttttttat ttttatttga ttatatttag attgctgatt ttccaagttc
      901 tgcgtgaagt ttgtccacgt ccggtgtcca ttcgtccact aggtcgactt ccagtcggcc
      961 atcttttcca aaattgtctt ccattgtttt ggcgactttc tgttccggct gggtttcgct
     1021 ttcaaatccg ccaaaagttg ttccatccat ggctcccttt ttgaccagat tctcaatggc
     1081 ggcccagacg gtttcgctat cccgtggaat aacaatggtt acttcgtcgc cggcatcggc
     1141 tttgaaatag gtgaccgaag cgagaaggac gcgaaaggct tttccttcgg caatgacgac
     1201 atagtttccg agatcatcct ggctgacgat gccgatcacg cgcttgcgct caatcgggcc
     1261 gatttgctta tagacaaaag aaccgtccgg agtgattgtc agcttgagca tatcaccttc
     1321 gaccaacttc gattttgaag cgtagttggc gggaataggg tattgctttc cgtcggttcc
     1381 aatcatgact tgtccgtcaa aaactccttc aacaatttca ccttcctcat cagaagaaac
     1441 gattttcggc gcccttttca ttgggacgcc ctcgatttgg gaaagcatgg cccgcgcgga
     1501 cgcaattgtc cgttcggcac cggcgatcat ttcccgaatc gcggcgatct tttgaagttc
     1561 ttgatctttg tcaatttcct tggttttctt ttttggcatt tatttctttg cggctgcttt
     1621 ggcatatggt caaaacaaaa atccccctat tccaaaacag ctgcttttta tttgtctttt
     1681 tgtattacat cctcattata caattccttt caaaacagtg caacagggca tatttcaggc
     1741 aaaattgtgg ataagtttgg ctttttcggt ccactagaaa aaatgttccg gaggactcca
     1801 aactcttgac aaaaaatatc gacgatgcta gattaataat acttagaata gctaatagtt
     1861 agataataaa agaataaaaa tatgaaaaaa agatcagcca ccgaaaagat catatctctg
     1921 atgtttaata tgggaagagc gatcaaaaaa caatgccttg aaagttgcgg acgggcggac
     1981 gggctctcca tccttcaaat cgaggctttg tggcatgtca aggaggggaa agaggtgctg
     2041 atgaaggatc tggcgagcca tcttttcatt gcgccgcctt cggctacgtc tcttgcggat
     2101 gatctggtga aagcgaaact ggtgaaaaga tcggaagaca aggaagacag gcgaaccacg
     2161 gccatttctc tgaccccgaa aggaaaacgg gcgcttgcga attttttgaa aaaacgaatg
     2221 gaaaaagcca aaaagaggat tgacaagctg acccaggcgg aaaaaaaatc tctgtttgcg
     2281 attttggaaa agctggcaaa agagaacgat caatcgagat aatcattgtt tcaggtaata
     2341 tattatgaaa aattttgcaa aatcaaaccg gcgagcactc acgatcgtgg cagtgatagc
     2401 cttgattggg gtttcagtct gggcggcctt ccatttcacg cggatcccgg ctttctccga
     2461 aacccagatt tttgaagtgc aaaagggcgc aatcaaaagt gaaatttcgg cggacatcac
     2521 tttgcagccg gcgacgaacg cagatctggc ttttgaaaac agcggaaaaa taaaaaatat
     2581 ctatgtttct gtcggtgatc aggtgaaaaa aggccaattg ctggcggaag aagtgaacag
     2641 cgattatgtc gtttctttgc gggaagcgga agcggcgcgc cgcgcggctc aagccgatct
     2701 cgagggagct caagaaaaca ttgatatcca aggagccaag ctcaagagtc tcaaaaatgc
     2761 aaacgcaaaa aaatatgatg tgaaggcaca gaaagaaacc aaagatcagg ccggctccag
     2821 cgctgactcc aaagaggcgc ttctcgaggc ggccaatcaa gcggtgattg ccgccaattt
     2881 gcaattggcg aaaacgcgcc tgacggctcc gatggatggg gtgatcacgg acaaatccat
     2941 cgaaatcgga gaagtggtct ctcaggccgg cccggtgatg aaaatcgctt ctggagataa
     3001 tcttgaggcg gacgcctatg tttccgaatt ggatgtgaaa aaaattgcgg tcgggggttc
     3061 ggcggaaatt gttttgagtt cggcggatgg aaataaaata accctggatt ccaaaataaa
     3121 gaatatctat cccgcagaaa ccgttcaaaa cggagtttct tcctacaaag ttgtgtttga
     3181 tcttgctgga caggaatcca atctcaaatc ggggatgact ggaatggcaa atataaagat
     3241 atccgatcag gcgggagcga tggttgttcc gcaaagttcg gttttttcag acgcgggaaa
     3301 aaagtatgtg atggtgatgt cgggtggttt tcctgaaagg aaagacgtac aagttggagc
     3361 atatggttct gatggcacag tcgaagttct ttcgggtctc gcggaagggg ataagatatt
     3421 aaaattctaa ctatatggaa caggaaaaaa acaatcaaat tgcggacagc cacagcacca
     3481 ccaaaggcga aaaaaagacc cgccggataa tgatctttgc gctggttgtc tttgctgtcg
     3541 cagcaagttt catcggcttg gtctatcttg atgccatatc aaagcgggtt tatgtcgata
     3601 agtccgaagt tgatgctcct cagattatgc tctcggcaaa gacgggtgga attttggaag
     3661 aattattcgt caaagaagga gatcagattt ctgcgaatga tgtcgtagca agagtcggaa
     3721 atgaattggt gaaatcgaaa gatgcgggct tggtgatcag ccggcaggat tcgatcggaa
     3781 aatattttgg tccgggagaa gaagttgtga cgatgatcaa gcctggagac ttgcgaatcg
     3841 ttggacggct ggcggaagac aagggactca aggacgtgaa agtcggacaa agagttgttt
     3901 ttgaagtgga tgcgttcggg acaaaacaat atgaaggcat cattgatgaa gtgagcccga
     3961 cttcccgggc atcggacgtc gttttcaata tttccgacca gagacaggaa aacgagttca
     4021 atgtgaaggc gcgcttcaat ctttcccagg atccagagct caaaaacgga atgtcggcca
     4081 aaatgtggat ctataagtag tataaatgcg cgatggctga caataacgga caaaaatcaa
     4141 cgatcggccg gtatcgctgg atgatactca ttaccgtcat tgtcggcacc tttttggggc
     4201 ggctggacca gacgatcgtc aacttggctt tgcccaaaat aatttccgac tatcggatca
     4261 cggtgacttc cgccgggtgg atcgccacgg cctatatcct ggccaacgcg atctttgttc
     4321 caatctgggg aaaactgggc gatacgatcg gaagaaaaaa agtatacctt tggggatttg
     4381 ccatctttat ttttggctcg gtcttggccg gttttgcctg gaatttgagc tcgctcattg
     4441 tctttcgcgt cattcaggcg attgccggtt ccgccgacta tcccaccgcg atggcgattt
     4501 tggccgtcac ctttccttcc ggaaaagaac gggcccaagc gctgggaatt tggtcctctt
     4561 cttttgccgc tgcggtcgtg tttggtccgc ttattggcgg accgctcatc gacaatttcg
     4621 gttggcgctc ggtttttctc atcaattttc cggtcgggat catcgggatc attatggctc
     4681 taacctatat caaggaatct gtttccgaaa aagtgaccaa atattttgac tggtggggcg
     4741 cgattacatt gggcgtatct cttgcttcgc ttgttttggt tctcgacaag ggcatgagtt
     4801 ggggatggac gagctgggaa agcctggttt catatttcgt gatgcttgtg tccctggcat
     4861 ttttcatccg ggtggaaaac aatcaccccg agccgatcgt cgatttcaaa tttttcaaga
     4921 ataacgtttt cacaaatact ttgatcaaca actttattgt tttcatggga atgatgggaa
     4981 gcgttttttt gattcccatt tttgcccaaa cctatctggg ctatgacgcg acgaagaccg
     5041 gatatctttt catcccgatg gccttcatga tcattttagc cgctcctttg ggcggcgcgc
     5101 tcaccggaaa agtccagccg cggtatgtta ttgcggtgag taccttcatt gcggcgattg
     5161 gcatctattt ttttacgggc attgatgctc gttcgcacgc aatgaacatc atttttccga
     5221 tggcagtgat ggctttcgga atcggtttcg ggatggccca gcggacgaat atcatcgcca
     5281 cggtcgtgcc gcagaatgaa atcggagtgg cttcgtcaat tttggcgctc gcgcgaaaca
     5341 tcgccggcgc gttcgggatc gcggtcttcg gtacgatact gaataactct accagttcga
     5401 aagttttgga aatcgcgcgc tatagttcac tgcacgcttt cactccgaat tttgtcggcc
     5461 aattcacatc actcatcatc ctcaaagcgc aaatcgaagc ctatcgaact gtatttttat
     5521 acgcctttgt ctccctcccc
//
LOCUS       DATKGS010000074         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_18512, whole genome shotgun sequence.
ACCESSION   DATKGS010000074 DATKGS010000000
VERSION     DATKGS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18512"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VMQ48_01835"
     CDS             <1..551
                     /locus_tag="VMQ48_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HUD08814.1"
                     /translation="DKMRYHRVIIMTDADVDGSHIRTLLLTFFYRNFEEIIKRGYLYI
                     AAPPLYRISKGKDVHYAYNDEDKNEIIEEFLQKSKEKKAVAAKAKKKSEPEKEEGEEE
                     ISDEEKKSGVNVQRYKGLGEMNPSQLWETTMDPANRVMYQVKVEDANEADKIFNILMG
                     SEVEPRKRFIQAEARSVVNLDV"
     gene            632..817
                     /gene="secE"
                     /locus_tag="VMQ48_01840"
     CDS             632..817
                     /gene="secE"
                     /locus_tag="VMQ48_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HUD08815.1"
                     /translation="MNKITTFVKEARVELRKVNWPTREQTIKYTALVVGLSLAVALFL
                     GALDYLFEYILRTFVIK"
     gene            819..1364
                     /gene="nusG"
                     /locus_tag="VMQ48_01845"
     CDS             819..1364
                     /gene="nusG"
                     /locus_tag="VMQ48_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HUD08816.1"
                     /translation="MAKQTQHHGRAWYAIHTYSGYEDNVAKNLKQRIESLDMQDKIFE
                     VMVPKERQIKIKNGKRTTIEERIFPGYVLVEMIVTDDSWYVVRNTPNVTGFIGLGTTP
                     TPIAEAEIESMKKRMGVAEPKYKIDVVKGDAVKIVDGPFKEFDGKVDAVDDARGKIKV
                     LVSIFGRETPVELDFLQIKKI"
     gene            1369..1794
                     /gene="rplK"
                     /locus_tag="VMQ48_01850"
     CDS             1369..1794
                     /gene="rplK"
                     /locus_tag="VMQ48_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HUD08817.1"
                     /translation="MAKAIKTVIKLQIKAGQANPAPPVGPALGQHGLNIQEFCTRFNE
                     ATKDKMGQVIPAEITVYEDRSFDFVLKTPPAADLLKKAAKAEKGAADPLKQKVGTVTK
                     AQIREIAELKMPDLNANDVEQAMKIIEGTARSMGIKVEK"
     gene            1807..>2338
                     /gene="rplA"
                     /locus_tag="VMQ48_01855"
     CDS             1807..>2338
                     /gene="rplA"
                     /locus_tag="VMQ48_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HUD08818.1"
                     /translation="MNITEAIAEIKKNAKAKFDESVEAHFRLDIDPKKSDQQVRGVAV
                     LPNGTGKSVKVVVFTTTQKKEAEAAGAELVGGEELIDKIKSSGKIDADVAIATPEMMP
                     KLATVAKILGPKGLMPNPKNQTVTPKVTEVIEALKKGRADFKNDNSGNIHQAIGKVSF
                     DAAKLEENFKALLEPSL"
BASE COUNT          736 a          496 c          624 g          482 t
ORIGIN      
        1 tggacaaaat gcgctatcat cgcgtgatca tcatgaccga cgccgacgtg gacggatcgc
       61 acatccgaac gctgcttttg acttttttct atcgcaactt cgaagagatc atcaagcgtg
      121 gttatctcta tatcgccgct ccgccgcttt atcggatttc gaaaggaaaa gatgtccatt
      181 atgcttacaa cgacgaggac aaaaatgaaa taatcgaaga atttttgcaa aaaagcaagg
      241 aaaagaaagc cgtcgccgcc aaggccaaga aaaaaagcga acccgagaag gaagaaggcg
      301 aagaagaaat cagcgatgaa gagaaaaaat cgggcgtgaa tgtccagcgc tacaaaggtc
      361 ttggagagat gaatccgtcc cagctttggg aaacgacgat ggatccggcc aatcgcgtga
      421 tgtatcaggt gaaagtcgaa gacgccaacg aagcggacaa gattttcaat attctgatgg
      481 gctccgaagt cgaaccgcgc aagcgcttca tccaagccga agcgagaagt gttgtgaatc
      541 tggatgttta ggatttcttg acagattttc tatatagtat attattgggt tatagaaaag
      601 cccgccgaag gctttttaaa aaagctttga aatgaacaaa atcacgacat ttgtcaaaga
      661 ggcccgcgtg gagctgcgga aagtcaactg gccgacgcgg gagcaaacga tcaaatacac
      721 cgcgctcgtg gtggggttga gtttggcggt ggcgctcttc cttggagctc tcgactattt
      781 attcgaatat atattaagaa catttgtgat caaataagat ggcgaaacaa acccaacatc
      841 atggccgcgc ctggtacgcg atccatacct attccgggta tgaagataat gtggccaaaa
      901 acttgaaaca gagaattgaa tccctggata tgcaggacaa gatttttgag gtgatggtgc
      961 cgaaagaaag acagatcaag atcaaaaacg gaaaaagaac aacgattgaa gagcggattt
     1021 ttccgggata tgtccttgtg gaaatgatcg tgacggatga ttcttggtat gttgtcagaa
     1081 acacgccgaa cgtgacagga ttcattgggc ttggaacgac tccgacaccg atcgccgagg
     1141 ccgagatcga atcaatgaag aagcggatgg gcgtggctga gccgaaatac aagatcgatg
     1201 tggtcaaggg cgacgcggtc aagatagtcg atggtccgtt caaagaattt gatgggaaag
     1261 tggacgcagt ggatgatgcc agaggaaaga tcaaggtgtt ggtttcgatc ttcgggcggg
     1321 aaacaccggt ggaactcgac tttttacaga taaagaagat ataaatatat ggcaaaagca
     1381 atcaaaacag tcatcaagct ccagatcaaa gcgggacaag ccaatccggc tcctccagtc
     1441 ggtcctgctc tgggtcagca cggcctcaac atccaggaat tttgcacgcg cttcaatgaa
     1501 gcgaccaaag acaagatggg tcaggtaatc ccggctgaga tcacggtata tgaagaccgt
     1561 tcgtttgatt ttgtgttgaa aactcctccc gcggctgatc ttctcaaaaa agcggccaaa
     1621 gcggaaaagg gggcggcaga tccattgaag caaaaagtgg gaacagtgac caaagcgcag
     1681 attcgcgaaa tcgccgagct caaaatgccg gatctcaacg cgaatgatgt cgagcaggca
     1741 atgaagatca ttgaaggaac agccagatcc atgggcataa aagttgaaaa ataattctca
     1801 attccaatga atatcactga agcaatcgct gaaataaaaa agaacgccaa ggccaagttc
     1861 gacgagtctg ttgaagcgca ttttcggctg gacattgatc cgaaaaaatc ggaccagcag
     1921 gtgcggggcg tggcggtgtt gccgaatgga acaggcaagt cggtgaaggt cgtcgttttc
     1981 acgaccacgc aaaagaaaga agccgaagcg gcgggagcgg aacttgtcgg gggcgaagaa
     2041 cttatcgaca agatcaagtc ttcaggaaag attgacgcgg atgttgccat cgctaccccc
     2101 gaaatgatgc caaaacttgc gacggtcgcc aaaattttgg gaccgaaagg tttgatgcca
     2161 aatccgaaaa accagacggt gacgccgaaa gtgacggaag tgatcgaggc gctcaaaaaa
     2221 ggccgggccg atttcaagaa cgacaattcc ggaaatatcc atcaggcgat cggcaaagta
     2281 tcatttgacg ctgcaaagct tgaggaaaat ttcaaagctc ttctcgaacc ttctctcc
//
LOCUS       DATKGS010000075         5563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_132718, whole genome shotgun sequence.
ACCESSION   DATKGS010000075 DATKGS010000000
VERSION     DATKGS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132718"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VMQ48_01860"
     CDS             <1..1257
                     /locus_tag="VMQ48_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUD08819.1"
                     /translation="EKASKQTLQWKSEKDLITNIRTHKSEIERLRMESDVLERQGDLA
                     RVAEIRYGKIPEFERKIKNEQRKLTQMQSKNPILKEEVTEEDIAGVVSRWTGVPVSKM
                     LEGEMEKLVAAEESLKTRVVGQDEAISAVANALRRSRAGIAEENRPIGSFIFLGPTGV
                     GKTELAKALAEFLFNDENAMIRVDMSEYMEAHSVAKMIGSPPGYIGHEEGGQLTEKVR
                     RRPYSLILFDEIEKAHPEVFNILLQILDDGRLTDGKGRTVNFKNTVIVMTSNVGSDII
                     YKMQEIGFKEEKRKEFVLKEDEMKERVLGSLREKFKPEFLNRVDEIIIFHPLAAEQIK
                     KIVDLQLELVAKRLKEKNIRIQITPAAREFLAREGFDPVYGARPLKRAIQNLVLDELA
                     LKIVEGKVKEGSKVKIDVSGGKILIK"
     gene            1274..2017
                     /locus_tag="VMQ48_01865"
     CDS             1274..2017
                     /locus_tag="VMQ48_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08820.1"
                     /translation="MFFSFNIFFLSVLFAANLWLSVSYIGYAGWFGILFSLIIIVAAR
                     IIVGRWKFTVLPAVLVPGAVLLLSLINPPFEMKFFIFLSTLAFYATVLAGWRLLRYEK
                     DETAKAMYNIATVAVLFCWYAASYGWYLNPNMSFPIWLLLVVFSVITFLVSYVSFATN
                     QIDREKWLIYSVFLTTLVAQTVWIQNFWPFGYLTVAVITLIIYCVGWEIVLSFFLKKL
                     TVRTVLFEILFLAGSVVLVLLSTKWYPVI"
     gene            2022..3218
                     /locus_tag="VMQ48_01870"
     CDS             2022..3218
                     /locus_tag="VMQ48_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUD08821.1"
                     /translation="MAENETMKGKTKKAKLAKFVFFVVLIFVVSGISSIFSDKYIFPW
                     LATQDWFKKQAFFKTAMDNVTVINKTEQVTVSENQDISGYTQKSTSSVVEIIERQKGD
                     KNVVSTSGQSASGVVVTSDGLVASYDNKFFGGKDAEYEAYFPDGKSYSAQVAAADPFS
                     NIVLLKLDNAENLPVAEFIAPEDIKTGIKTAVVGRSGFNAEISLLLGITSEWGKSYSL
                     SGPLASSEKLQGVLFADFAGAASTDSKALAGGAVVDQNGNMLGILGESKDDSGVKMFV
                     VPINHLQYVIDQYLGKGKIERATLGVYYVALSKENAYFARNGFDHGALVYSPSGQQGL
                     AVLAGSSADQAGIKVGDIILSVNGDEVNPDQNLAQLISEYKPGDMVSLKIVRDGKEIE
                     VKVSLN"
     gene            3412..3717
                     /gene="rplU"
                     /locus_tag="VMQ48_01875"
     CDS             3412..3717
                     /gene="rplU"
                     /locus_tag="VMQ48_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HUD08822.1"
                     /translation="MLAVIKTGGKQYLVKKGDKIKVETLEGEIGDKIEFSEVLFLGDE
                     KEVKVGAPFLKDVKIEGKILKQGKAKKVVGVKHKAKKRYKVKFGHRQNFTEVEITKI"
     gene            complement(3751..4341)
                     /gene="recR"
                     /locus_tag="VMQ48_01880"
     CDS             complement(3751..4341)
                     /gene="recR"
                     /locus_tag="VMQ48_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HUD08823.1"
                     /translation="MFPKSFQKLIDHFAALPSVGPKMAERLVLYLWKYDQEKLDDFSE
                     IIKNLKRNIHFCKQCFNISENGLCAICQDKKRDGSIICVVEDPLDVISLEKTRRMRGL
                     YHVLGGTINAPVDKNHPLKIKELLARLKKEKISEVIIATNPTTEGEATALYLARFIKP
                     LNIKTTRLARGLPTGGDIEYADEATLGGAIEGRKEI"
     gene            complement(4331..>5563)
                     /gene="dnaB"
                     /locus_tag="VMQ48_01885"
     CDS             complement(4331..>5563)
                     /gene="dnaB"
                     /locus_tag="VMQ48_01885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HUD08824.1"
                     /translation="PDDFYKDANRLVYETMLELYEEREPIDVLSLSNKLEEKKQLEKI
                     GGASYLTEIVNAVPTASNVVHYAKVVQKKSLLRKLITAASDIVELGYEEAEDVEKVLD
                     EAEQKLFKVSQKYVKADFVPLKTYLESAFNRIDELHKGDKELRGVPSGFADLDSVLAG
                     FQKSDLIIIAARPSIGKTALALDIARNVAVQKKIPVGIFSLEMSSDQLVDRMLSAQGK
                     VDAWRMRTGHLVSNDREDDFAKIGEAMGALSEAPIFIDDAASANVMEMRTMARRLQAE
                     HNLGLIIIDYLQLMQGRSTDNRVQEVSEISRSLKQLARELNIPVIALSQLNRSVESRS
                     PQVPKLSDLRESGSIEQDADVVMFLYREDREKPDTPNKNVIQVIVSKHRNGPLGTANL
                     YFNDAITSFSSLEKKHVS"
BASE COUNT         1482 a         1142 c         1456 g         1483 t
ORIGIN      
        1 gaaaaagcca gcaagcagac tttgcaatgg aaaagcgaga aggatttgat caccaatatc
       61 cgcacgcaca aaagcgagat tgagcggctg cgaatggaat cggacgtgct tgagcggcag
      121 ggagacctgg cgcgcgtagc ggaaattcga tatggaaaaa ttcccgaatt tgaaagaaag
      181 atcaaaaacg agcaaagaaa actcacccag atgcagagca aaaatccgat tctcaaagaa
      241 gaggtgactg aagaagatat tgcgggagtg gtttcccgct ggacgggcgt gcctgtttcc
      301 aaaatgctcg aaggcgagat ggaaaaattg gtggctgccg aggaaagtct caaaacgcgg
      361 gtcgtcgggc aggacgaagc gatcagcgcg gtggcgaacg ctttgcgccg cagccgagcc
      421 gggattgccg aggaaaatcg accaattggt tccttcatct tccttgggcc gacaggcgtg
      481 gggaaaacgg agttggcgaa ggctttggcc gagttcttgt tcaacgatga aaacgcgatg
      541 atccgggtgg acatgagcga atatatggaa gcgcacagcg tcgccaaaat gatcggttcg
      601 cctccgggat atatcggcca cgaagaggga ggacaactca ccgaaaaagt gcgccgccgg
      661 ccgtacagcc tgattttgtt cgatgaaatt gaaaaagccc atcccgaggt cttcaatatc
      721 ttactccaga ttcttgacga cggtcgtctc actgatggca agggaagaac ggtcaatttc
      781 aagaatacgg tgatcgtgat gacatctaac gtgggatctg atataatcta taaaatgcag
      841 gaaatcgggt tcaaagaaga gaaacgaaaa gagtttgtgc tcaaagaaga cgaaatgaaa
      901 gagcgggtgc ttggctctct gcgggaaaaa ttcaagccgg aatttttgaa ccgggttgat
      961 gagatcatta tttttcatcc gctggcagcc gagcagatca agaaaatcgt agatttgcag
     1021 ctcgaacttg ttgccaagag actgaaggaa aaaaatataa gaatccaaat cactcccgcg
     1081 gcgagagaat ttttggcccg tgaggggttt gatccggtct atggtgcccg tcccctgaaa
     1141 cgggcgatcc aaaacctggt tctcgatgaa ctagccctca aaatcgtgga aggaaaagtg
     1201 aaagagggaa gcaaggtgaa gatagatgtc tctggtggaa aaattttaat taaataactt
     1261 tatatttttt aggatgtttt ttagtttcaa tatatttttt ctctccgtgc tgttcgcagc
     1321 caatctttgg ctgtcagtga gctatattgg ctatgccggc tggttcggga ttctcttttc
     1381 gctgatcatc atcgttgcgg ccaggatcat tgtcgggcga tggaagttca cggtcttgcc
     1441 ggcggttttg gttccgggag cggtgcttct tttgtcgctc attaatccgc ctttcgagat
     1501 gaagtttttt atctttcttt ccacgctggc tttttatgcg actgttttgg ccggctggcg
     1561 actattgcgc tatgaaaaag acgaaaccgc caaggctatg tacaacattg ccaccgtggc
     1621 tgttctcttt tgctggtatg cggcgtccta cggctggtat ttgaatccga atatgtcgtt
     1681 tccaatttgg ttgctgcttg ttgttttttc tgtcatcact tttctcgtgt catacgtttc
     1741 ttttgcaaca aaccaaatag atcgagaaaa atggcttata tacagcgtat ttttgacgac
     1801 tttggtcgcg caaaccgtct ggatccaaaa tttttggccg ttcggatatt tgacagttgc
     1861 cgtgataact cttataattt attgcgtcgg ctgggagatc gttctcagct tttttctcaa
     1921 gaaactcact gtccgaaccg tcctatttga aatactattt cttgctggat ccgtcgtgct
     1981 tgtccttctc agcacaaaat ggtatccggt gatataaacc aatggcagaa aatgaaacca
     2041 tgaaaggaaa gaccaaaaaa gcaaagctcg cgaagtttgt tttttttgtc gttcttattt
     2101 ttgtcgtttc agggatcagc tcgatttttt ccgacaaata tatttttcct tggctcgcca
     2161 cgcaagattg gttcaaaaaa caggctttct tcaaaacggc catggacaat gtgacagtta
     2221 tcaacaagac cgaacaggtg acagtgagtg agaatcagga tatttccggc tatacccaaa
     2281 aatcaacatc aagtgtcgtt gaaatcatcg aaaggcaaaa aggagacaaa aatgttgttt
     2341 ccacttcggg acaatcggca agcggagtgg ttgtcacctc ggacggactg gtggcaagct
     2401 atgacaataa atttttcgga ggaaaagatg cggaatatga agcatatttc ccggacggaa
     2461 aatcatacag cgcgcaagtt gcggccgcgg atcctttctc gaatatcgtt ttgctaaaat
     2521 tggataatgc ggaaaatttg cccgtggcgg aattcattgc tccggaggat atcaaaacag
     2581 ggataaaaac agctgtggtt ggccggagcg ggttcaacgc ggagatatcg ctcttgttgg
     2641 gaatcacgag cgagtgggga aaatcatatt ctctttcagg tccgctggcg tcttcagaaa
     2701 agttgcaggg agtgctgttt gctgattttg cgggagcggc gagcacggac agcaaggcgc
     2761 tcgcaggcgg agcggtcgtt gaccaaaacg gaaatatgct tgggattttg ggtgagagca
     2821 aggatgattc cggcgtgaaa atgtttgttg tgccgatcaa tcatcttcaa tatgtgattg
     2881 accaatatct cggaaaagga aaaatcgaaa gagcgaccct tggagtctat tatgtggcgc
     2941 tgtcgaaaga aaacgcctat tttgcgcgca atggttttga ccacggcgcg ctggtatatt
     3001 ctcccagcgg ccagcaagga ttggcggtgc tcgctggaag ctccgctgac caagcgggaa
     3061 tcaaagtcgg ggacatcatt ttgagtgtga atggagatga agtgaatccc gatcaaaact
     3121 tggcgcaact gatttcggaa tacaagccgg gggatatggt gagcttgaag atagtgagag
     3181 acggaaaaga aattgaagta aaagtaagtt tgaattaatt ttaaagacta aatttgaaat
     3241 tttaaatcaa ttttaaatta tcaaatgact aaatttttaa catttaaaat ttagacattt
     3301 agaattcatt ttaaatttaa aattgaaaat ttagaattgc aatcgttgac atttctattt
     3361 gtatctagta gtctgttaag gtgctaatga aataataaac ttaaaaatat catgctagca
     3421 gtcatcaaaa ctggcgggaa gcagtatctc gtcaagaaag gggataagat caaggttgaa
     3481 actctcgaag gcgagatcgg agataaaatc gaattttccg aagttttgtt tttgggtgac
     3541 gaaaaagaag tgaaagtcgg cgcgcctttc ttgaaagacg tgaaaataga aggcaagatt
     3601 ttaaaacagg ggaaagccaa gaaagtggtg ggcgtgaaac acaaggccaa gaaaagatac
     3661 aaagttaaat ttggccatcg acagaacttc acggaagtgg aaataacgaa aatctagata
     3721 tagaaaaacg ggcatcgggt gcccgttttt ctatatttct tttcttccct caatcgctcc
     3781 gccgagggtc gcctcgtccg cgtattcgat gtcgccgccc gtcggcagtc ctcgcgccag
     3841 gcgggttgtt tttatattca aaggttttat aaaacgcgcc aagtataatg ccgtcgcttc
     3901 gccttcagtt gtcggattcg tggcgatgat cacttcggat atcttttctt ttttcaatcg
     3961 ggcgagcagc tctttgatct tgagcgggtg atttttgtcg acgggagcat tgatcgttcc
     4021 gcccaggacg tgatagagtc cgcgcatccg gcgggttttt tcgagagaaa tcacatcaag
     4081 cggatcttca acgacgcaga taatggaacc atctcttttt ttgtcttggc aaatagcgca
     4141 caacccattt tcagatatat tgaagcattg tttgcagaag tgaatgtttc gcttgaggtt
     4201 tttgataatt tcggaaaagt catccagctt ttcctgatcg tatttccaca aatacaaaac
     4261 cagccgttcg gccattttgg gaccgacgga aggcaaagcg gcgaagtggt ctatcaattt
     4321 ctgaaacgat ttaggaaaca tgttttttct cgagtgaaga gaagctggtg atagcgtcgt
     4381 tgaaatacag attggcggtt cccaagggtc cgtttctgtg tttggacaca atcacttgaa
     4441 tcacgttttt gttcggcgtg tccggttttt cgcggtcttc gcgatagaga aacatcacaa
     4501 cgtcggcgtc ttgttcgatc gatcccgatt cacgcaggtc ggaaagtttc ggaacttgcg
     4561 gactgcgaga ttccacggag cggttgagct gggaaagagc gatgacggga atattcagct
     4621 cgcgggcaag ctgcttcaat gaccttgata tctccgaaac ttcctggacg cgattgtctg
     4681 ttgaacgtcc ctgcatcagc tggagatagt cgatgatgat gagaccgaga ttgtgttcgg
     4741 cctggagccg gcgggccatc gtgcgcattt ccatcacgtt ggcgctggcg gcatcgtcaa
     4801 tgaagatcgg ggcttcggac agcgcgccca ttgcttcgcc gatcttggca aaatcatctt
     4861 cccggtcgtt gctcacgaga tggccggttc gcatccgcca ggcatccact tttccttggg
     4921 cggaaagcat acggtcaacc aattgatcgg atgacatttc gagggaaaat attcccaccg
     4981 gaattttttt ctgcacggcg acattgcggg cgatatcaag ggcaagggca gtttttccaa
     5041 tggaggggcg ggcggcgatg atgataagat ccgatttttg aaaaccggcc agaacgctgt
     5101 cgagatccgc aaatccggag ggcaccccgc gcagctcttt gtcgcctttg tggagttcat
     5161 cgatgcggtt gaaagcggat tcaagatagg ttttgagcgg gacgaaatcg gcctttacgt
     5221 atttttgcga gaccttgaaa agtttttgtt cggcttcgtc gaggactttt tccacatcct
     5281 cggcttcttc atagccgagt tcgacgatat ctgatgcggc agtgatcaat tttcgcaaca
     5341 aagatttttt ctggacgact ttggcgtagt ggacaacatt cgaagcggtc ggaacggcgt
     5401 tcacgatttc ggtcaaatag ctcgcgccgc cgattttttc aagctgtttt ttttcttcaa
     5461 gcttgttgga aagggaaagg acatcgatcg gttcgcgctc ttcatagagc tcgagcatcg
     5521 tttcatagac gaggcggttc gcgtctttat agaaatcgtc cgg
//
LOCUS       DATKGS010000076         4237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_108474, whole genome shotgun sequence.
ACCESSION   DATKGS010000076 DATKGS010000000
VERSION     DATKGS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108474"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1519
                     /gene="dnaX"
                     /locus_tag="VMQ48_01890"
     CDS             23..1519
                     /gene="dnaX"
                     /locus_tag="VMQ48_01890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HUD08825.1"
                     /translation="MKSGRIGQAYLFAGPRGTGKTSIARIFAKTINCTSLKNGEPCLK
                     CDSCQNILGNRALDIIEIDAASNTGVDNIRELRETVKLPPSSLKYKVYIIDEVHMLSG
                     GAFNALLKTLEEPPANVVFILATTEVHKIPETILSRVQRFDFTRLTIEEIINRLDEIA
                     KLEKVKVDREALEIIASSSEGGMRNAESLLGQIIALEDKKITTKEVEFILGTVSEKFT
                     AEFVDLIISQRYPEVFSKIGELREMGIDFKNFGKSLLNYLRQMLVLKINPDLGQKLSY
                     EITKERLGQIKSQIASVETDEILNFINILQENTEKFKSSPIPQLFLEVSIAKIASQNT
                     TVPPAQPQDNIKKKIITEPQSPALKKEEKNSAEKSPVKPAVIEKKEAENKNNSADIDL
                     VISNWSEIIESLKSYNHSLSSVLKMSSPTLIDGDYLILKTKYDFYKEKLVEMTNRLTI
                     EKAIDKITGVDLKLKSLTEKEFVDLYPDFKEKKSLMQEAVDIFGGKIV"
     gene            1527..1598
                     /locus_tag="VMQ48_01895"
     tRNA            1527..1598
                     /locus_tag="VMQ48_01895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1559..1561,aa:Gln,seq:ttg)
     gene            1655..1855
                     /locus_tag="VMQ48_01900"
     CDS             1655..1855
                     /locus_tag="VMQ48_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08826.1"
                     /translation="MEQDLLEKLNQLEQKVEETRVLAQKTWRLFFWTMVGTVVMFVLP
                     LIALAFVIPYFLKSVTAGLNGL"
     gene            1857..2435
                     /locus_tag="VMQ48_01905"
     CDS             1857..2435
                     /locus_tag="VMQ48_01905"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUD08827.1"
                     /translation="METNESIIKKTKDGKIGVIPTDTIYGVAAAAFSKKAVTRAYKIL
                     KRNPKKPFIILISSIKDLSLFGIKLDRDTGKILHSLWPGKISVVLPTPSKKFQYLHRG
                     TKNLAFRLPRDTNLARIIKKTGPLISTSANPEGAKPAETIAEAKAYFRNSIDFYVDGG
                     KIKSLPSTLVEIKDGKVNVIRKGAGKISLIKV"
     gene            2450..2572
                     /locus_tag="VMQ48_01910"
     CDS             2450..2572
                     /locus_tag="VMQ48_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08828.1"
                     /translation="MEEKCPGCGQPMESCTCGSDEKESHCGCSHCGHPCGPEEK"
     gene            complement(2634..3902)
                     /locus_tag="VMQ48_01915"
     CDS             complement(2634..3902)
                     /locus_tag="VMQ48_01915"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HUD08829.1"
                     /translation="MIYDLRIKKIISSLPKSPGVYEFRDKQGKILYIGKATSLRDRVG
                     SYFLKNANLDRPNIVPMVLQIADIKIHEIDSVLEALILESNLIKKHQPKYNVMAKDDK
                     SFGYFVITKEKFPRVIILWKTELGKIPAKKIYGPYLSKYQMNIALKLIRRIFPFHSNK
                     QQTEKGCLDFQIGKCPGPHSGAISQKDYLKNIQGIEMILQGKKKNLLIEIKKQMKLSA
                     KKDEFEKAAEARNRIFALEHIRDVALIANDENFQFPISNFQKMRIEGYDISNIAGDYA
                     VGSMVVFSGNKPDKSQYRKFKIKTISGIDDVGMMREVLYRRFNNNWPKPDLMILDGGK
                     GHLNMAQKVIGDLKLGIPIIAVAKGPTRKRLDLYQSKFLLTFLGKNEELRKNYSNVLS
                     DIRLLEHIRNEAHRFAISYHKKLRKNWKER"
     gene            complement(3906..>4237)
                     /locus_tag="VMQ48_01920"
     CDS             complement(3906..>4237)
                     /locus_tag="VMQ48_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUD08830.1"
                     /translation="EQRIKLASELSRRMTGNTLYILDEPTTGLHFDDVKKLLDVLHRL
                     VDAGNTVIVIEHNMDVIKTADWIIDLGPEGGDKGGRVVVTGPPEEVVKYHKESYTAKF
                     LREVLNK"
BASE COUNT         1247 a          952 c          891 g         1147 t
ORIGIN      
        1 gaggtgagaa ggaggaaacg ccgtgaaatc ggggcggatc gggcaggctt acctttttgc
       61 ggggccgcgg ggaacgggaa aaacttcgat tgcgaggatt tttgccaaaa caataaactg
      121 cacgagctta aaaaacggcg agccgtgcct caaatgcgat tcgtgccaga atattcttgg
      181 caatcgggcg cttgatatta tcgaaatcga cgccgcttca aacacgggag ttgataatat
      241 ccgcgaactg cgcgaaaccg tgaaattgcc cccgagctcg ctgaaataca aagtctatat
      301 catcgacgaa gttcatatgc tctccggtgg ggccttcaac gcgcttctca aaactctcga
      361 ggagccgccg gcgaatgtcg tcttcatttt ggccacaacc gaagtccaca aaataccgga
      421 gactatttta tcaagagtcc agcgatttga tttcacgaga ctgacaatcg aagaaatcat
      481 caaccgcctc gacgagatcg ccaagcttga aaaggtgaaa gttgaccgcg aagcgcttga
      541 gattatcgcc tcttcctcgg aaggcggaat gcgaaacgcc gaaagtttgc tgggacaaat
      601 catcgccctt gaagacaaga aaatcaccac caaagaagtg gaattcattt tgggaaccgt
      661 ttcggaaaaa ttcacggcgg aatttgtaga tttgataata agccaaagat atcccgaagt
      721 tttttcaaag atcggcgaac tgcgggaaat gggcatcgat ttcaaaaatt ttggcaaatc
      781 cctgctcaat tatctgcggc agatgcttgt cctcaagatc aatcctgacc tcggtcaaaa
      841 actttcttat gagatcacga aagaaagact gggtcagatc aaaagccaga tcgcgagcgt
      901 cgaaaccgat gagatcctga atttcatcaa catccttcaa gaaaataccg aaaaatttaa
      961 atcaagcccc atcccccagc tttttcttga ggttagtatc gcaaaaatag catcgcaaaa
     1021 tacaacagtc ccgccagctc aaccccagga taacataaaa aaaaagatta taactgaacc
     1081 gcaatcccct gctctgaaaa aagaggaaaa aaacagtgct gaaaaatctc cagttaaacc
     1141 ggctgtgata gaaaaaaagg aggcagaaaa taagaataat tctgccgata ttgatctcgt
     1201 catctctaac tggtccgaga tcatcgaatc gctgaaaagc tacaatcatt ccctctcctc
     1261 cgttctcaag atgtcgagtc ccacgctcat tgatggagat tatctgatcc tcaaaacaaa
     1321 atacgatttt tacaaggaaa agctggttga aatgacgaac cgattgacaa tcgagaaagc
     1381 gattgataag ataacaggcg ttgatttgaa gctgaaatcc ctcacggaaa aagaatttgt
     1441 cgacctttat cccgacttca aagagaaaaa aagtctcatg caagaggcgg tggatatatt
     1501 tggaggaaag atagtctaag tacgattgcc cggtcgccaa gcggtaaggc agcggccttt
     1561 gaagccgcta ttcgtaggtt cgaatcctac ccgggcaaca agataatttg gatatggata
     1621 tgcttgaaga aaaaataaat atttgcaaaa atatatggag caagatttgt tggaaaaatt
     1681 gaaccagctt gagcaaaaag tcgaagaaac ccgcgttttg gctcaaaaaa cctggaggtt
     1741 gtttttttgg acgatggttg gaaccgttgt gatgtttgtc ctgccactca ttgccttggc
     1801 ctttgtcatc ccctacttct tgaaatctgt cactgccggt ctcaatggcc tataatatgg
     1861 agacaaacga atcgataata aaaaaaacaa aagacggaaa aatcggtgtt attcccaccg
     1921 atacaattta tggcgtcgcc gctgcagcat tttcaaaaaa agccgtcacg cgtgcataca
     1981 aaattctcaa gagaaaccca aaaaagccgt ttatcatcct catttcttcc ataaaagatt
     2041 tgtctttatt cggaataaag cttgatcgcg atactggaaa aatattgcat tccttgtggc
     2101 ctgggaaaat aagcgtggtt ttgccaacac cgtccaagaa atttcaatat cttcaccgag
     2161 ggacgaaaaa tttggctttc aggctgccga gagatacaaa tttagccaga atcatcaaaa
     2221 aaaccggccc tttgatctca accagcgcca atcccgaagg tgccaaacca gcggaaacaa
     2281 tcgccgaggc caaagcatat tttagaaaca gcattgactt ctatgttgac ggtggtaaaa
     2341 taaagtcctt gccatcgact ttggttgaaa taaaagatgg taaggtgaac gtaataagaa
     2401 aaggagcggg caagataagt ttaattaaag tttaatattg aacaaaaata tggaagaaaa
     2461 atgccctggc tgtggacagc cgatggaaag ctgcacctgc ggttcggacg aaaaagagtc
     2521 ccactgcggt tgttcccact gcggacatcc ctgcggcccg gaagaaaaat aacgcgtcct
     2581 cttaaacaaa aaaaaccctg cactttgcgg ggttttttgt ttttggctaa tcgctagcgc
     2641 tctttccaat tttttctcaa cttcttgtga tagctgatcg cgaaacgatg ggcttcgttg
     2701 cggatgtgtt cgagcaacct tatgtcactc aaaacattac tataattttt tcttagctct
     2761 tcgtttttgc ctaagaatgt tagcaaaaat tttgattgat ataaatcaag cctttttcgg
     2821 gtcgggcctt tggcaaccgc gataatagga attcctagtt ttaaatcacc aatcactttc
     2881 tgcgccatat tcaagtgtcc ctttcccccg tcgagaatca ttaaatccgg ttttggccaa
     2941 ttgttgttga atcttctata caaaacttcg cgcatcatcc cgacgtcgtc gatcccggat
     3001 atcgttttta ttttgaattt tcgatattgc gatttgtctg gcttattgcc cgagaataca
     3061 accatcgatc caacggcata atcgccggct atgtttgaga tatcatagcc ctcaattctc
     3121 attttttgga aattggaaat tggaaattgg aaattctcat cgtttgcgat gagtgccaca
     3181 tcccgaatat gctccagcgc aaagatccga ttccgcgcct cggctgcttt ttcaaactca
     3241 tcctttttag cagataattt catctgcttt ttgatttcga taagaagatt tttctttttg
     3301 ccttgaagga tcatctcgat gccttggata tttttgagat aatctttttg ggatatcgct
     3361 cctgagtgcg gcccgggaca tttcccgatt tgaaaatcaa ggcagccttt ttccgtttgt
     3421 tgcttattgg aatgaaacgg aaaaatcctt cggatcaatt tcagggcaat attcatctga
     3481 tatttcgaaa gatacggccc gtatattttc ttggccggaa tttttcccag ttccgttttc
     3541 cagaggatga ttacgcgtgg aaacttttct ttggtgatca caaaatagcc gaagctcttg
     3601 tcgtctttcg ccatcacgtt gtatttcggt tggtgttttt tgatcagatt tgattccaaa
     3661 atcaaagcct caagaaccga gtcgatctcg tgaattttga tgtcggcgat ttgcaaaacc
     3721 atcggcacaa tattcggccg atccagattc gcatttttga gaaaataact ccccacccga
     3781 tcccgcaaag aggtcgcttt tccaatataa agaattttcc cttgcttatc acgaaattcg
     3841 taaacaccgg gagatttggg gagactggat attattttct taattcttaa atcataaatc
     3901 atgaatcatt tgttcaaaac ttctctcaaa aacttcgccg tataactttc tttgtgatat
     3961 ttcaccactt cttccggcgg gccggtgacg accacacggc cgcctttgtc gcccccttct
     4021 ggccccagat cgatgatcca atcggcggtt tttatgacat ccatattgtg ttcgatcaca
     4081 atgaccgtgt tccccgcatc caccaagcgg tgtaaaacat ccaaaagctt tttcacgtca
     4141 tcgaaatgta gtccggtggt cggctcgtcg agaatatata aggtattccc cgtcatgcgc
     4201 cgcgaaagtt ccgacgctag cttgatccgc tgctccc
//
LOCUS       DATKGS010000077         8136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_50628, whole genome shotgun sequence.
ACCESSION   DATKGS010000077 DATKGS010000000
VERSION     DATKGS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50628"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VMQ48_01925"
     CDS             <1..1256
                     /locus_tag="VMQ48_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HUD08831.1"
                     /translation="YPGANLTDSIIVASINPKTYQTALLSIPRDLYVQIPDTNSYTKI
                     NALYARAQDKDASGKSGIDDLEKTIADITGQPIDYYIALDFDGFKQIINELGGIKVQV
                     PKDLHDERYPGPNYSYETFDIKAGLQNLDGETALKFTRTRHDEDGDFGRAYRQQLILE
                     SARSKAFSLGTILNLPEVSGLLDTLGQHLRTNIQIDELNSFLDLIGKIDTHTTTNKVL
                     DAGKPDSVMAVSHVSLGGVRAFILIPRTGNYDETQDIAKNIFDLELIERKKAAIKLED
                     ASVTIINNSGVSGFDGKLKDLLDKFGYTTSISQIKATKTPYRSNSNTTNDTTVYDLTG
                     GIKPFSLEDISKKLGAKISIYLPDSLSAQCQGADFCLVAGPDITDKLNYEENSVQDLE
                     NGYNQQQVDEKTYFNLLKRGSNQKL"
     gene            1277..1825
                     /gene="pth"
                     /locus_tag="VMQ48_01930"
     CDS             1277..1825
                     /gene="pth"
                     /locus_tag="VMQ48_01930"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUD08832.1"
                     /translation="MKTIFIAGLGNPGKEYEKTRHNAGFMFANHFFEKFRDDFGFSEW
                     KFNKKLKSEISIGKIEKNKIILLKPQTFMNLSGEAVSAAKKYYKVKLENIFVAHDDID
                     LLLGKYKVSINSSSAGHNGAQNIIDLLGTKNFTRIRIGVENRGDKKIPTEKYVLGKFT
                     AAETKVISKILPLVSDELIKNI"
     gene            complement(2349..4133)
                     /gene="typA"
                     /locus_tag="VMQ48_01935"
     CDS             complement(2349..4133)
                     /gene="typA"
                     /locus_tag="VMQ48_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HUD08833.1"
                     /translation="MEIRNIAIIAHVDHGKTTITDAIMRQTGIAGEEVATMDSDALEQ
                     ERGITIYSKNTSIFYKDTKINIVDTPGHADFGSEVERVLRSIDSVLLVVDAQEGPMPQ
                     TRFVLKKSLELGLKPIVVINKIDKPAAHPEIAQEQVFELFLDLGASDEQLDFTVVYAI
                     GREGIAKKSLDDNSKDLTPLLDTILEKVPAAISNSEAPFRMQPFSLGYDNFLGRLAIG
                     RIYEGTIRSGEVFVKKPTGEVRKGKIIKMFNFEGISRKEISESVGGDTVMIAGIPDID
                     IGETICANADQEPLPAIKIDEPTITLNFLVNDSPFAGRDGKFVTSKQIKERLEKELEV
                     NVGLKVDFSNGDFYKVSGRGELHIAILLENMRREGYELQVSQPQVIIKEENGKKLEPF
                     EEATIDVPEQFSGTIIETLGKRKGIMVNMKQEGSQVRMVFEIPTRGILGYRNQFMIET
                     RGEGIFASRFLEFRPHAGAISKHATGSMASMVAGKALAFSLANLQDRGTLYIGANTEV
                     YEGMVIGNTAKGNDMAVNPIKGKNLTNMRSSGADAAIHLVPHMELTIERGFEIMADDE
                     YLEVTPQSVRLRKQILNETKRVQAGRNK"
     gene            4623..5729
                     /gene="dprA"
                     /locus_tag="VMQ48_01940"
     CDS             4623..5729
                     /gene="dprA"
                     /locus_tag="VMQ48_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HUD08834.1"
                     /translation="MEGKNQNEIIFWHAFNCVDGFGPQAFKKLLAGFSNLEEAWKSTN
                     VDKLQQLGLSKKQIDNFFRFRQKHSPDILFEELAKENIEILTIKDEAYPSQLKEISSA
                     PPVLYLRGDKSTLSNKSIAIVGSRKFTEYGQRVTENLTRDLVRAGLTIVSGLALGIDG
                     IAHRAALDAGGITAAVLGTGIDDATIYPREHFNLAQRIIESGGSLITEQPPETPSLKQ
                     NFPARNRIMSGLALGTLVIEAAESSGSLITANFALEQNREVFAVPGDIFSPQSAGANM
                     LVKHGAKLVTSAADILEELSISRIQPELPLKIFEPKTDEEKIIWKNLSNEPLHIDKIS
                     KMTRLDTAVVSSVLGMMEIENVVKDVGGKNYIKL"
     gene            5819..8047
                     /gene="topA"
                     /locus_tag="VMQ48_01945"
     CDS             5819..8047
                     /gene="topA"
                     /locus_tag="VMQ48_01945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HUD08835.1"
                     /translation="MKLIIVESPTKAKTISKFLGPKFKVMSSYGHIRDLPEKKMGIDI
                     EKNFAPQYIVNPKSEEKAELLKKAAKTAEAVILASDEDREGEAIAWHLSEILKLKPDE
                     KNRIVFHEITKKAIEKALDNPRSINFDLVDAQQGRRVLDRLVGFELSPFLWDKIRRGL
                     SAGRVQSIALRIIAEREREIEKFKKEEFWTIAAMLKKSGNKNSFETSLTRQNEKKIEE
                     AITLKLFAGDYKTKKSVIADKKTADEINAELEKAKYAVSEITKKEITRTPPAPFTTST
                     LQQAAINSLGYSAKVTMMLAQKLYEQGYITYMRTDSTNLSLESMLAARKTIEKVFGKN
                     YTLESPRFYKTKSKGAQEAHEAIRPTNPGQTPDDLSKLDDRQLRLYGLIWRRMLASQM
                     QPAVLNSVKVLIDAKGEKNNYTFQANGSTVKFDGYLKVYGAKMPVTENLLPDLKEKEA
                     LDLEKIIPAQKFTTPPPRYTEASLVRVMEEYGIGRPSTYAPTISTIIDRKYVEKNEEK
                     KLEPLEIGLLVNDLLVEHFPQIVDMKFTAQMEENLDEVAQGLKKWVPVIKEFYDPFHK
                     NLETKSGEVKKEDLMQKLGRQCPQCGGDLIMKFGRFGKFIACMKYPECKYTEKTAEEQ
                     KEEDENSGEVCEKCGAPMMVKRGRFGAFLGCSKYPECKTIKSIAKKTGVKCPKCHEGD
                     ILERKSKKGRLFFGCSKYPACDFSLWNKPTGETCPKCQGLLTFQAKVKVKCGNKECGY
                     EK"
BASE COUNT         2384 a         1980 c         1832 g         1940 t
ORIGIN      
        1 actatcctgg agccaacctt actgactcga tcattgtcgc cagcatcaac ccgaaaacct
       61 atcaaacagc ccttctgtcc atcccccgcg acctctatgt ccagatcccg gacacaaatt
      121 catacacaaa aataaacgcg ctctatgccc gggcgcagga caaagacgct tcgggaaaat
      181 ccggcattga cgaccttgaa aaaacgatcg ccgacatcac cggccaaccg atcgactatt
      241 atatcgctct cgatttcgac ggcttcaagc aaatcatcaa cgagctggga ggcatcaagg
      301 tccaagttcc gaaagacctg cacgacgagc gctatccggg tccgaactat tcctatgaaa
      361 cttttgacat caaagccggg cttcaaaatc tcgacggcga aacggccttg aaattcaccc
      421 gcacccgcca cgatgaagac ggcgatttcg gccgggcgta tcgccagcag ctcatcctcg
      481 aatccgctcg ctcaaaagct ttttctttgg gaacaattct caaccttccc gaggtgagcg
      541 gtttgctcga tacgcttggg cagcatttgc gcaccaatat tcaaatcgac gagctcaatt
      601 cttttttgga tctcattggc aagatcgaca cacatacgac gaccaataaa gtcctcgacg
      661 ccgggaaacc cgacagcgtg atggccgttt cgcacgtttc cctcggagga gtccgcgctt
      721 tcattttgat cccccgcacg ggaaactatg acgaaacgca ggacattgct aaaaatattt
      781 tcgaccttga actcatcgag cggaaaaaag cagccatcaa gcttgaagat gcctctgtaa
      841 cgatcatcaa taacagcggt gtctctggat ttgatggaaa actgaaagat cttcttgaca
      901 aattcggcta cacgacgagt atcagccaaa tcaaagccac caagacgccc taccgaagca
      961 attccaacac tacgaatgat acgactgtct atgacctcac gggcggaatc aaacctttct
     1021 cccttgaaga tatttccaaa aaactcggcg cgaaaatttc gatctatctg ccggacagcc
     1081 tttccgccca gtgccaaggc gccgattttt gcctcgtcgc cggaccggat atcacagata
     1141 aactgaacta cgaagaaaat tccgtccaag accttgaaaa cggatacaat cagcagcaag
     1201 tggacgaaaa aacatatttc aacctgctga aaagaggaag taatcagaag ctttagattt
     1261 atgctaaaat aaccctatga aaacaatctt catcgccggg cttggaaacc ctggaaaaga
     1321 atacgaaaaa acccgccaca atgccgggtt tatgttcgcg aaccactttt tcgaaaaatt
     1381 tcgggatgat ttcggcttct cggaatggaa gtttaataaa aagttaaaat ccgaaattag
     1441 cattggaaaa attgagaaaa ataaaataat attgctcaaa ccccagactt tcatgaatct
     1501 ttcgggtgaa gcggtttcgg cggcgaagaa atactataaa gtgaagctgg aaaatatctt
     1561 cgtcgcccac gatgatattg atttgctgct tggaaaatac aaagtttcca tcaactcatc
     1621 ttccgccggt cacaatggcg cgcagaacat cattgacctg cttggcacaa aaaacttcac
     1681 tcgaatacgg attggagttg aaaatcgcgg agataaaaaa atccccaccg aaaaatatgt
     1741 gttgggaaaa tttactgcgg cggaaacgaa agtgatttcg aaaatattgc ctctggtttc
     1801 tgatgaactt ataaaaaata tctaacttta aatcatcctc ccctgccaag gggaggtgcc
     1861 cgaagggcgg aggggttttt gcaatgaaaa gattcaataa tcttaattgg tacagaaatt
     1921 tccgaaagga tctccgcaat aatgcgacgc cccaagaaac aattctttgg gcaagattga
     1981 gaagaagcca attgggattc aagttcagga gacaacactc aattgggaaa tatatagttg
     2041 atttttgttg cccaagcaag agtctgataa tcgaggtcga tggtggacat catgacgaag
     2101 taaatcagag aagatacgat gaaagcagaa ccaaatattt tgaaagtctg ggttataaag
     2161 tacttcgatt ttgggacaat gacgttaata ataatttgag tagtgtggtt tcagaaataa
     2221 tcaataatct ggaataaaac cacccctcga tcccctcctt gaaaaaggag gggaggctat
     2281 aggaaaaaaa tattcaacat aaaaaatccc cctctgaagg gggataaatt atattcaaaa
     2341 taaatttttt atttatttct ccccgcctgg actcgtttcg tttcgttaag gatttgtttt
     2401 cgaagacgca cgctttgggg cgtgacttcg agatattcgt cgtccgccat tatttcaaag
     2461 ccgcgctcaa ttgtgagttc catatgcggc accaggtgga tcgccgcgtc agcgcccgat
     2521 gaacgcatat tcgtcaaatt ttttcctttg atggggttca ctgccatatc atttcctttc
     2581 gctgtgtttc cgatcaccat gccctcatat acttccgtgt tcgcaccgat atagagcgta
     2641 cctcggtcct gaagattggc aagtgaaaag gccagggcct tcccggccac cattgaagcc
     2701 atcgaaccgg tggcgtgctt ggaaattgct cccgcgtgcg gccgaaactc gagaaaacgg
     2761 ctggcaaaaa ttccttcgcc gcgtgtttcg atcataaatt ggttgcgata tcccaaaatg
     2821 ccgcgggtcg gaatttcaaa aaccatgcgg acttggctgc cctcttgttt catattcacc
     2881 atgatcccct ttctttttcc gagagtttca atgatcgttc ccgaaaattg ttccgggaca
     2941 tcgattgttg cttcttcaaa aggttccagc ttcttgccat tctcttcttt gatgatgacc
     3001 tgcggctgag aaacctggag ttcatagccc tcacggcgca tattttcaag caagatagcg
     3061 atgtgcagtt cccctcttcc gctcacttta taaaaatcgc cgttggaaaa atcgactttc
     3121 agtccgacgt tcacttcgag ttctttttcg agacgttctt tgatttgttt cgacgtcaca
     3181 aacttcccat cgcgccccgc gaatggcgaa tcgttcacca gaaaattgag ggtgatggtc
     3241 ggctcatcaa ttttgatcgc cggaagcggt tcttgatcag cattcgcgca aatagtttca
     3301 ccgatatcaa tatcaggaat acccgcaatc atcaccgtgt ctccgccgac ggattccgaa
     3361 atttcttttc gcgagatgcc ctcgaagttg aacattttga taatttttcc tttgcgcact
     3421 tcgccggttg gcttcttcac aaacacttcg ccgctgcgga ttgtgccttc atatatcctt
     3481 ccgatcgcga ggcgtcccaa aaaattgtca tagcccaagc tgaagggctg catccgaaaa
     3541 ggcgcttcgg aattggaaat tgccgccgga actttttcaa ggatcgtatc aagcagcggc
     3601 gtgaggtctt tggagttgtc atccaagctt tttttcgcga tcccttcgcg gccgatggca
     3661 tagacaaccg tgaaatcgag ctgctcgtca ctcgcgccga ggtcgagaaa aagttcaaag
     3721 acttgttcct gggcaatctc gggatgcgcc gccggcttgt cgatcttgtt tatgacgaca
     3781 atcggcttca agcccagctc caaagatttt ttgagcacaa atcgggtttg cggcatcgga
     3841 ccttcctgcg catccacaac caaaagaacc gaatcgatcg atcgcaaaac ccgttccact
     3901 tccgatccaa aatcggcgtg cccgggcgta tccacgatgt tgatctttgt gtctttgtag
     3961 aatatggaag tgttttttga atatatggtg ataccgcgct cctgttcgag cgcgtcgctg
     4021 tccatcgtag caacctcttc gcccgctatt ccggtctgac gcatgatggc atctgtgatt
     4081 gtcgttttgc catggtcgac gtgggcgatg atggcaatgt ttctaatttc catatgagtg
     4141 ttgtagacgt cggacgtcta caaaaataaa aaccgcgcga gagcggcaaa tttctgaacg
     4201 aattagctcc gggactgttg gtcttttgtg atattagcag gaaaatgaga tagtgtcaaa
     4261 aaaatccaga acaaaatccg gcgaagccgg actccaaaac ttttcccttt tcatttttaa
     4321 ttttaaactt tcgctgtcct ctgtcccacc tgaaagtgcc gaattgagag catttgctct
     4381 ctttccgaac ttacccaacg atgattaagg aggggtcaca ccgtcggact tccagacggg
     4441 acagaagaca cgaatttgct ttatatctag ataaatagaa cattttctga attcccctat
     4501 ccagaagtga tattatagca cttacacgag atttgtcaat atgcggaata tttcacccgt
     4561 agagacgagg caatgcctcg tctctacgat gattacgcaa agttgtgtta ttatattcag
     4621 gtatggaggg aaaaaatcaa aacgaaataa ttttctggca cgcattcaat tgcgtggacg
     4681 gtttcgggcc gcaagcattc aagaagctac tggccggttt ttcaaatctt gaagaggctt
     4741 ggaaaagcac caacgtcgac aagctgcagc aactcggcct ctcaaaaaaa caaatcgata
     4801 atttttttcg attccggcaa aagcattcgc cggatattct ttttgaagag ctcgcgaaag
     4861 aaaatattga gattctcact ataaaagacg aagcctaccc gagccaactc aaagaaatct
     4921 cttcggcgcc tccagtactt tatctccgcg gagacaaaag tacactttcc aacaaatcaa
     4981 tcgcaatcgt cggatcgcgc aaattcactg agtacggcca gcgagtgacg gaaaatctca
     5041 cccgcgattt ggtgcgcgcc gggctcacga tcgtgagcgg actagcgctg ggaatcgacg
     5101 gcatcgctca tcgtgccgcg cttgacgccg gtggaatcac cgctgccgtt ctcgggacgg
     5161 gaatcgacga cgcgaccata tatccccgcg aacatttcaa tcttgcccag agaatcatcg
     5221 aaagcggcgg atcgctaatc acagaacaac ctcccgaaac cccgtcgctc aaacaaaatt
     5281 ttcccgcccg gaaccgcatc atgtccggac tcgcgctcgg gacgctcgtg atcgaagctg
     5341 ccgaaagttc gggatcgctc atcacagcga atttcgccct tgagcaaaac cgcgaagttt
     5401 tcgccgtgcc gggagatatt ttttctccgc aatcagctgg tgcgaatatg ctcgtgaaac
     5461 atggagcaaa attggtcacc tccgcagcag atatcctcga agaattgagt atctcccgca
     5521 tccaacccga gcttccgctc aagatcttcg agcccaaaac cgatgaagaa aaaatcatct
     5581 ggaaaaatct ctcgaatgaa ccgcttcata ttgacaagat ttcgaaaatg actagactgg
     5641 acacagcagt cgtgtcatcc gtgctcggaa tgatggaaat tgaaaacgta gtgaaggatg
     5701 tgggagggaa gaattatata aagttgtaaa ttttataatt tcagaatttc agaattttca
     5761 aacaatttct aaattttgaa atttgagaat tgttttttaa attgaaaatt aaaaaattat
     5821 gaaattgata atagtagagt ctccgaccaa agcgaaaacc atctccaaat tcctcggacc
     5881 gaaattcaag gtcatgtctt cgtatggcca catccgcgac cttccggaaa agaaaatggg
     5941 cattgatatt gaaaaaaatt tcgcgcctca atacatcgtc aatccgaaat cggaagaaaa
     6001 agccgaactg ctcaagaaag cggccaaaac cgcggaagcg gtgatcctcg caagcgacga
     6061 agaccgcgaa ggagaagcga tcgcttggca tctttcggaa attttgaaat tgaaaccgga
     6121 cgaaaaaaac cggatcgttt ttcacgagat cactaaaaaa gccatcgaaa aagcgctcga
     6181 caacccacgc agcattaatt ttgatctggt ggacgcccaa caaggacgcc gcgtactcga
     6241 ccgcctggtc ggtttcgagc tttccccttt tctttgggac aagatccggc gcggcctctc
     6301 cgccggacgc gtgcaatcga ttgctctgcg catcatcgcc gaacgggaac gggaaatcga
     6361 aaaattcaaa aaagaagaat tttggacgat cgcggccatg ctgaaaaaat ccggcaataa
     6421 aaattctttt gagaccagcc tcactcgcca gaatgaaaag aaaatcgaag aagcgatcac
     6481 gctgaaactt ttcgcgggag attataagac gaagaaatcg gtcattgcag acaaaaagac
     6541 ggcggatgaa atcaacgcgg aactggaaaa ggcgaaatat gcggtgagtg aaatcacgaa
     6601 aaaagaaatc acgcgcactc ccccggcgcc tttcacgact tcaaccctcc aacaggccgc
     6661 catcaattcg ctcggatact cggcgaaagt gacgatgatg ctggctcaga aactatatga
     6721 acagggctac atcacctata tgcgaacgga cagcacaaat ttatcgcttg aatcgatgct
     6781 cgccgccaga aaaacgatcg aaaaagtttt tggaaaaaat tatacattgg aaagtccgcg
     6841 cttctataaa accaaatcaa aaggcgcgca ggaagcgcac gaagctatcc gtccgacaaa
     6901 tcctggccaa actcccgacg acctctctaa actggacgat cggcagcttc gactgtatgg
     6961 tctgatttgg cgaagaatgc tggcctcgca aatgcaaccg gccgtgctga actcggtgaa
     7021 agttttgatc gatgccaagg gcgagaagaa taactacact ttccaagcga atggatcgac
     7081 cgtgaaattc gacggatatt tgaaagtcta tggcgcgaaa atgccggtta cggaaaatct
     7141 tctgcccgat ctcaaggaaa aagaagcgct cgatctggaa aaaataatcc cggcccaaaa
     7201 atttacgact cccccgccca gatacaccga agcgtcactc gttcgcgtga tggaagaata
     7261 cggcatcggc cggccgtcga catacgcgcc gacgatctcg acgatcatcg accgcaaata
     7321 cgtcgagaaa aacgaagaaa aaaaacttga gccgttggaa atcggacttt tggtgaacga
     7381 tcttttagtt gaacacttcc cgcagatcgt ggatatgaaa ttcacggctc agatggaaga
     7441 aaatctcgac gaggtggcgc aaggtttaaa gaaatgggtg ccggtcatca aagaattcta
     7501 tgatccgttc cacaaaaatc tggaaacgaa atccggcgag gtcaaaaaag aagacctgat
     7561 gcaaaaactc ggccgccagt gtccccagtg cggcggcgat ctgatcatga aattcggccg
     7621 gtttggaaaa ttcatcgctt gcatgaaata tccggaatgc aaatatacgg aaaaaactgc
     7681 ggaggaacaa aaagaagaag atgaaaacag cggcgaagtt tgcgaaaagt gcggcgcgcc
     7741 gatgatggtg aagcggggtc gcttcggagc gtttctgggc tgcagcaaat atccggaatg
     7801 caaaacgatc aaatcaatcg ccaagaaaac gggcgtgaaa tgtcccaagt gccacgaggg
     7861 cgacattctc gagcgcaaat caaaaaaagg ccggctcttt ttcggctgct cgaaatatcc
     7921 cgcctgcgat ttttccctct ggaacaaacc gacgggtgaa acttgcccca aatgccaagg
     7981 cctgctgact ttccaagcca aggtcaaagt gaaatgcggc aacaaagagt gcgggtatga
     8041 gaagtaattt ttgcttttta taaagcaaat tttcgcactt gacagatttt tttattctga
     8101 tagtattata ttaataaggc tgggattccc cccacc
//
LOCUS       DATKGS010000078         3685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_108660, whole genome shotgun sequence.
ACCESSION   DATKGS010000078 DATKGS010000000
VERSION     DATKGS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108660"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..2877
                     /gene="uvrA"
                     /locus_tag="VMQ48_01950"
     CDS             91..2877
                     /gene="uvrA"
                     /locus_tag="VMQ48_01950"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUD08836.1"
                     /translation="MANKSHLIVKGARVNNLKNISVKIPRDKIVVITGLSGSGKSSLA
                     FDTIFAEGHRRYVENLSAYARQFLASVKKPEIEKIENLSPVIAIDQNSVNRSPRSTVG
                     TMTELYDWLRILFARLGTPHCPKCSRELSQKTASEIADVILSKKKGEKIQIAILAGPK
                     NKTKSSAEILSSFEQMGYARVRLNGKIVPATQARFEIGKNSEPDKIEAVVDRITLDKN
                     FSDRERLVDSIETAMKASGGEVTISFPGEEDAKYNRYLICSDCGVSISEITPRHFSFN
                     SPEGACSACAGLGTKLEVDIDQVMPNKNLSIREGAIKPWNITGVRNGAASRLEQLLNN
                     LSESLRFSLDAPVKKLDKKVLDTVLHGGRDFEGVVPALEKKYKEASSDFSRGEAEKYM
                     IVKTCPACKGRRLKKESLAVKISGSSIADFCETSLDRLKEKLGGIEKIDGIPDFGKQM
                     AREILAETGKTIHNLCEAGLGYLTLARNANSLSGGEAQRVRLAVQIGSGLTGILYILD
                     EPSIGLHERDTEKLIGVFKKLQSEGNSVIVVEHDAQIMRCADWIIDMGPGAGEAGGEI
                     IFSGPAKKMQTAETQTAKYLNGKNKVFVKKTFRKGNGKKITIVEASENNLKDITVSIP
                     LGKLSVITGVSGSGKSSLVSDIVGKALRKHFFRAKDQPGAHERILGLEYISKAIEIDQ
                     SPIGRTPRSNAATYTGVFSHIRDIFASLPEARRLGLNATYFSFNMKGGRCEVCQGEGQ
                     KKIEMHLLPDIYIPCEACGGTRFSQKVLTVEYQGANVASVLDMSVDYAWRFFQNHPLV
                     SEKLETLNRVGLGYLKLGQSAMYLSGGEAQRIKLATELARRTNGKALYILDEPTIGLH
                     FGDVAKLLKILDELVEKGNTVLIVEHNLDVIRAADWVIELGPEGGEAGGKLVFEGTPD
                     KLKKAKTWTGKYLR"
     gene            2980..>3685
                     /locus_tag="VMQ48_01955"
     CDS             2980..>3685
                     /locus_tag="VMQ48_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08837.1"
                     /translation="MAENSSTLGEVVEALESDELKNHIEEFRGFYFLEGRKNLVERRI
                     QCDKNSAVKFEIAERAAWWLRFVPYVRMIAVTGTLAMKNCEKNSDIDFFVVLEKGRIF
                     TGRILVTLVVHLLGKRRHGKKIKNRICLNYFTTTGNLEIERQDLFAANEYSFIYPIFG
                     YNIFQKFCGINLEWIKKIKPNWQVPELKPARYCVEHSQPGRSLQRLFENLINMLWGDR
                     IESWLKRHQITRIERNP"
BASE COUNT         1042 a          807 c          964 g          872 t
ORIGIN      
        1 ggggaggact ttactttttt tgatttatcc tgtacaattc acatctagtt tggtcttata
       61 acctcactta ataatattgt taataatatt gtggcaaaca aatcgcatct tatcgtcaaa
      121 ggcgcgcggg tgaataacct caaaaatatc tcggtgaaaa ttccgcggga caaaatcgtg
      181 gtgatcaccg gcctttcggg ttcgggaaaa tcgtctctcg cattcgacac tattttcgcc
      241 gaggggcaca gaagatacgt tgagaatctt tcggcttatg cccggcagtt tctggcgtcc
      301 gtcaaaaaac cggaaattga aaagattgaa aatctttcgc cggtcatagc gatcgaccaa
      361 aacagcgtga atcgcagtcc gcgttcgacc gtcgggacga tgaccgaact ctatgactgg
      421 ctgcgcatcc ttttcgcccg gctcggaaca cctcattgtc cgaaatgctc aagggagctt
      481 tcacaaaaaa cggcatctga aattgccgac gttattcttt ccaaaaaaaa aggagagaaa
      541 atccagattg cgattttggc cggtccgaaa aacaaaacga aatcatccgc cgaaatcctt
      601 tccagttttg agcagatggg atatgcccgg gtaagactca atgggaaaat cgttccggca
      661 acccaggctc ggtttgaaat tggaaaaaat tcggaacccg acaagattga agcggttgtc
      721 gaccgaatca ccctcgacaa aaatttttcc gaccgcgagc ggctcgttga ttcgatcgaa
      781 acggcgatga aagcgagcgg aggagaagtg acgatcagtt tcccgggcga agaagacgca
      841 aaatataacc gatatcttat ctgctcggat tgcggtgttt ccatttcaga gatcactccg
      901 cgccattttt ccttcaacag tcccgaaggg gcgtgcagcg cgtgtgcggg ccttggaacg
      961 aagcttgagg tggatatcga ccaagtgatg ccgaacaaga atttgagcat ccgcgaggga
     1021 gcgatcaaac cttggaatat caccggcgtt cgcaacggtg ccgcttcccg tctcgaacag
     1081 ctgctcaata atttgtccga aagccttcga ttttcgctcg atgctcccgt gaaaaaactc
     1141 gacaaaaaag ttttggacac ggtccttcac ggcggacgcg attttgaagg agtggttccg
     1201 gcgcttgaaa aaaaatacaa agaagccagc tccgattttt cccggggtga agcggagaaa
     1261 tatatgatcg tgaaaacctg cccggcgtgc aaaggaagac ggctcaagaa agaatcactg
     1321 gcggtgaaaa tttccgggtc ttcaattgct gatttttgcg aaaccagcct tgaccggctc
     1381 aaagagaaat tgggtggaat cgaaaagatc gacggaattc ctgattttgg caaacaaatg
     1441 gcccgggaaa ttctcgcaga aaccggaaaa actattcaca atctgtgcga agcggggctg
     1501 ggatatctca ctctcgcgcg caatgccaat tccctctcgg gaggtgaggc gcagcgggtg
     1561 cggctggcgg tgcagattgg atcaggtctc acgggaatcc tctatattct ggacgagcca
     1621 tcaatcgggc ttcacgaacg ggatacggaa aaattgatcg gtgtcttcaa gaaacttcag
     1681 agcgaaggga acagcgtgat tgtggtcgag cacgacgcgc aaatcatgag atgtgccgat
     1741 tggatcatcg atatggggcc gggcgcggga gaagcgggag gagagattat cttttccggt
     1801 cccgccaaaa aaatgcagac ggccgagacg caaacggcga aatatttgaa cggaaaaaac
     1861 aaagtttttg tgaaaaaaac tttccgaaag ggcaatggga agaaaatcac aattgtcgaa
     1921 gcttcggaaa acaatttgaa agacatcacg gtttcgatcc cgcttggaaa attgtccgtg
     1981 atcacaggcg tttcggggtc gggaaagagt tcgttggtga gtgacatcgt tgggaaagct
     2041 ttgcgcaagc atttttttcg cgccaaagac cagcccggag cgcacgaacg gatcttgggg
     2101 cttgagtata tttcaaaagc gatcgagatc gaccaatcgc caatcggacg tactccgcgg
     2161 tcaaatgcgg cgacgtatac gggagttttc tctcatatcc gcgatatttt cgcttcgctt
     2221 cccgaagcgc gccgacttgg cctcaatgcg acgtatttca gtttcaacat gaaaggtggg
     2281 cggtgcgaag tctgccaggg agaaggacaa aaaaagatcg agatgcatct cttgccggac
     2341 atttatattc cctgcgaagc ctgtggcggc acgaggttca gccaaaaagt tttgacggtt
     2401 gaatatcaag gggcgaatgt tgcttcggtg ctggatatga gcgtcgacta tgcctggcga
     2461 ttttttcaaa atcatccgct agtctcggaa aaactggaaa cactcaaccg ggtcggacta
     2521 ggatatctta agctcggcca gtcagcgatg tatctctcag ggggtgaagc gcagaggatc
     2581 aagctagcaa ctgaacttgc ccgccggaca aacggaaaag cgctctatat tctcgacgaa
     2641 ccgacaatcg gccttcactt cggcgacgtg gccaaacttc tcaaaattct cgacgagcta
     2701 gttgaaaagg gcaatacggt tttgatcgtc gagcacaact tggatgtcat ccgcgcggct
     2761 gactgggtca ttgagcttgg tccggaaggt ggagaagcgg gcgggaaatt ggtttttgaa
     2821 ggcacgccgg ataaactcaa aaaagcgaag acgtggacag ggaaatactt gagataatgt
     2881 atcatgcccg atatccttcc aaaaaatatc ttatcaacaa tttcttatta cgacatttta
     2941 aatttccccc tgacttcctt tgaagtctgg aaatatcttt tggcggaaaa tagctcaaca
     3001 ctcggagaag ttgtcgaagc gctggaaagc gacgaattga aaaatcatat cgaagaattt
     3061 cgcgggttct attttttgga agggcgcaaa aatttggtgg agcgcagaat ccaatgcgac
     3121 aaaaattcgg ctgttaaatt tgagatcgca gagcgtgcgg cttggtggct cagatttgtt
     3181 ccatatgttc ggatgatcgc cgtgacgggg acgctcgcga tgaaaaattg cgagaaaaat
     3241 agcgacatag atttttttgt ggttttggaa aagggcagga tattcaccgg acggatactg
     3301 gtcacgctag tcgttcactt gcttggcaaa cggcggcatg gaaagaagat aaaaaaccgg
     3361 atttgtctca attatttcac tacgaccgga aatcttgaaa ttgagcggca agatcttttc
     3421 gcggcgaatg aatattcttt tatatatccc atatttggat ataatatttt tcagaaattc
     3481 tgcgggataa atttggaatg gatcaaaaaa ataaaaccga attggcaggt gccggaattg
     3541 aaaccagcca gatattgcgt tgagcacagc cagcccggca gatctctcca aagattattc
     3601 gagaacttga taaacatgct ttggggtgat agaattgaat cctggctgaa gcgtcatcaa
     3661 attacgcgga ttgagcggaa cccgc
//
LOCUS       DATKGS010000079        10557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_32781, whole genome shotgun sequence.
ACCESSION   DATKGS010000079 DATKGS010000000
VERSION     DATKGS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32781"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2190)
                     /locus_tag="VMQ48_01960"
     CDS             complement(<1..2190)
                     /locus_tag="VMQ48_01960"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HUD08838.1"
                     /translation="MDAYSLNLILYPLAGLAALVCFVSGALLVFKSFVGFRGIITRSL
                     NMDLDVVKVSRPMKREAQGGEQQHPKSEKELISVMEHLFASLGNIKEKSNIWHRIIYG
                     DAVVALEMANPADNDEISFYVGAPKKFINIVERQIHSFYPKASIERVKDYNIFSPGSF
                     TAASSLTLKDKHHFPIRTYQALEADPLNNITNVLGKFDEKKEGAAIQLLIKPARSGWR
                     QKGKLVAHKMQQGRQLSDVKRDTIARQVGRGLLSVFHQKKDKQEDILKQNKDVIQLTP
                     EEQEVVKKIEGKAGKSGFSVNIRLVASALTQERSEVILSELENGFIQYENPGLNSFKV
                     LRESKRRPKNIAFDFIFRNFRDDQSFILSSEEVASVFHFPLFSTETPKIAWLKARTAA
                     PPSNISKDGITLGFNDYRGVETVIRHAEDDRRRHFYVVGQTGTGKSTMMEEMVKQDIK
                     NGKGVCVIDPHGEFVDHVLECVPKERAEDVIYFDPADMERPYGLNMLEFDANKPEQKT
                     FVINEMINIFDKLYDLKQTGGPMFEQYMRNAALLVMEHPDSGSTLMEIPRVLADEEFR
                     HLKLSHCTNPVVKNFWEKEAEKAGGEAALANMVPYITSKLTQFISNDMMRPIIAQEKS
                     TINFRQAMDDQQIILVNLSKGKIGEINANLLGMIIVGKILIGALSRVDISESERKDFY
                     LYIDEFQNFTTDSIAQILSEARKYRLALIIAHQFIAQLKEEISKAVFG"
     gene            complement(2353..2823)
                     /locus_tag="VMQ48_01965"
     CDS             complement(2353..2823)
                     /locus_tag="VMQ48_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08839.1"
                     /translation="MAQSNYQEPIEQKDLDLAKKLREANIDAGKKETIPTPEVSKEVQ
                     VETIAESPEEKVSEKIETARQTIGTQQATPAANIASDAESVSGIEEFEKKVEKLVEIA
                     IQKGPEHAIQVAQHMDKGKDPAAADNYTLDEIHDRLMEDELRAQLMQKGLLKEL"
     gene            complement(2968..3318)
                     /locus_tag="VMQ48_01970"
     CDS             complement(2968..3318)
                     /locus_tag="VMQ48_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08840.1"
                     /translation="MPQDTILQQSIIEELGLQDLPKEKQEEILVKMTEVLLKKIYLET
                     FEKLGEADREELAKMLDTEEAEPEKVEAYLRTKIEDYDGFVKKIVEDFTEEMKKTTGD
                     LGKIKEEELAKVGV"
     gene            complement(3343..6114)
                     /locus_tag="VMQ48_01975"
     CDS             complement(3343..6114)
                     /locus_tag="VMQ48_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08841.1"
                     /translation="MEKIPELNIEEQLKKDREELKEAGMLPKDSFPTGAGFMGAEVDL
                     DAREAEEVKARKTGTADKKEKEPEFSPEELRAIQDTAYYIQQHRMSNDPERQKYWAKK
                     YGLDENIFLKENIKKILSGKGEWNDEAQKFMQNWDMETAKKAFKNMKKAGEQMLEPDS
                     GSKTGTLPDKTPMEDALKKQAKENQRQIDNVSIEDARMKGAENAEEARKMEFEAMNSL
                     EALKADLEAKRMLAARYDVDMSGRWNKLKKVLGFNSKEIKDSGVEEKKNEYKQALKRY
                     LDAKRESLGAVTKEQAAQLYAEFTVGEATLFLEAKTDESAKMRKERGEWPDKVMDGYM
                     KVVDKWRGLSFGKKMAVSGALLAGGIVSGAYLGGIFVGLAATAKVGQRIVSGGATGVG
                     LKKLMDTVSAKRENKAALEEAGKILSQDDWMKALNAKIDGEIGTIDTRIGKMESKDLT
                     RKRVATFAGIFVGSGAMAQIINGTGLGGFIKDKASGVFGNVKHFAERILSPDQAYAAE
                     LAPAGGGTIATQAHEGLVGYGSEASGHEFVQAYPEGKYGLGLYGSEGEGHDLVDAHPE
                     GKYGSGLYGTEGEGHEAKAVNPEEKSSLIGYGAEGDGHVADIRDIASVKIKAGGNMWG
                     SIENNIKANPSAYGLDPNNPNFTKDMHRMTKQMLDEFAYRKGMSYEDLDQIARTKLRA
                     GDTFKIIYNPTTEEISLDDFHGKAFGSDVLHGSAAEEMATMRPTVTPDVSHGGAAGAV
                     HENTLNKPHLSHGTVKHGTGVAEHQPARGAGKTMQYDADTPEQINAKKEFALADKYQK
                     DLRVFNAQETARLEGVNEAANDQLFSSTKEGIEKIFHESGISIRSNILDKPMNAWEKL
                     IATENYITQDVQDADAVGKMNINLNKLKYLRLILGRYNTSGSETIGDCLKQAVKNPKI
                     LYLINKSVL"
     gene            complement(6119..7084)
                     /locus_tag="VMQ48_01980"
     CDS             complement(6119..7084)
                     /locus_tag="VMQ48_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08842.1"
                     /translation="MGKGNPHRQSGVGRNAGKGWKMEQILESGELALPKVSTRKEKGD
                     KKADIIDLSSKRPVDASSIHFGPNIPKPVEKKSESQPESVKAENKKSYYPKDDLKYWS
                     GDDNEMLGAVVETLEKTREAKLAEVETKIEKEFDPAEKRFIDPMIDDYPSYFYGEKEK
                     ADRRKVRIDENWVEAIRQFYIDERKRWGLNRKIAPEKAELLGNYLELKLPVGFPEKKI
                     KSMNNKDKKETEFTPNQSSELLQWIGLTSKRFKEWVKESEKWGSEFGDEETKKKAIFE
                     QIKEKMNSVMSSDSEMKKLFSEEEIPRAVEHVMKYVEKFNFDTKK"
     gene            7276..9072
                     /locus_tag="VMQ48_01985"
     CDS             7276..9072
                     /locus_tag="VMQ48_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08843.1"
                     /translation="MLEKYQYYIVAGILGIMLVLSISVALGDAAIMDEVAHIPSGYSY
                     IHFHDYRLNPEHPPLLKDLAGIPLQFLKVTFPTDKNYWTTDANGQWDAGSTFLYLPGN
                     NTEAILFWSRFPIILIALALGFMIFKWAKDLFGTKAGLFALTLYAFDPNVLGHDHYVT
                     TDLGIAAFSLFAFFAFIKYLKNPNWKTITLAGIFLGLAALAKFSSVLLFPIFGLSLIV
                     YALARKEKLSFSFLGSGKIKKFWVQKLYLYITTFIVIFAIHGLLIGLVYELNIYKMPT
                     EKIHQLIDTQLSPNGIQKIAAPYLHKMADNTVLRPYSQYLVGFFMVFGRVEGGNTTYF
                     FGQVTNQSFRAYFPLVFLIKESLPLLILLLFSIAVGLTILLKKNLPSFPKKIWTNIKD
                     YFDGHIAEFSMLSFIALYWYVSITGNLNIGLRHLFPTLPFMYILIAKEVTIFGNRAAK
                     KYQKYFWTIVLLLVAWLAAEVAFAYPNYLAYFNETVGGPKNGYKYVTDSNVDWGQDLK
                     RLEKFVSDNNIQKIRVDYFGGGDVVHYLGDKAIMWHSDLGQEPGWYAISATFLQNSLY
                     YKITEGKPDYEWLRERTPTAEIGGSILIYKID"
     gene            complement(9075..9956)
                     /locus_tag="VMQ48_01990"
     CDS             complement(9075..9956)
                     /locus_tag="VMQ48_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HUD08844.1"
                     /translation="MPIFSKKKKNKIVQKSWQRKNTKAYPGASKKETSQRGGIFSRFI
                     FWLLLVVFLGICVYLLFFSTFLDVNKINIQGNQDISSVEISKVVERSLEGKYLKYLPK
                     KNFFLVSVSNIGSAVKNNFNRLEVASIGKKFPDTIVVKVVERKAELVWCSGGACYLVD
                     SSGLAYGGANGTEEDLRAGNFLVVVDDSAIPVEIGKTKISPDFIGYIEKVNAMIRDDL
                     KLDVAASYHTPGIASQEVSVVTNEGWTLKISSEYSVDEAKKIIQTLFEKDLNGETRKN
                     LDYLDLRVKGKVYYKTK"
     gene            complement(10014..>10557)
                     /locus_tag="VMQ48_01995"
     CDS             complement(10014..>10557)
                     /locus_tag="VMQ48_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HUD08845.1"
                     /translation="IGLPLAILYLESGESSFWKWWKILVQAKMKALFSRKFPSKLVLE
                     LGVEKKGDMAYLLEMVRPSWVVVTNIEGSYSDAESNLDDIFEEMKILVKKMPSSGKLI
                     LNFDDERVKKLANFSPSEKISYGFSDGAKAQASNLKTTVQGQTFDFSFQGKHESVEIK
                     KYGRHNVYAWMIAKIVKEIL"
BASE COUNT         2488 a         2721 c         1915 g         3433 t
ORIGIN      
        1 tccaaacacg gctttcgaaa tttcctcttt gagttgggca atgaattgat gggcgatgat
       61 gagcgccagg cgatattttc gcgcttccga aaggatctgg gcgatcgaat cggtcgtgaa
      121 attttgaaat tcatcgatat agagatagaa gtctttgcgc tcactttctg aaatatcaac
      181 ccgcgaaagc gccccaatca aaatttttcc aacgatgatc attcccagga gattggcatt
      241 gatctcccca atctttccct ttgaaagatt gaccagaatg atttgctggt cgtccatagc
      301 ctggcggaaa ttgattgtcg atttctcctg cgcgatgatc ggccgcatca tatcattcga
      361 gatgaactgc gttagcttcg aagtgatata cggaaccata ttggccagag ccgcttcccc
      421 gcccgccttt tccgcttctt tttcccagaa atttttgacg accggattcg tacaatgtga
      481 aagcttcaaa tgccggaatt cttcatcggc caggactcgc ggaatctcca tcaatgttga
      541 accggaatct gggtgttcca tcaccaagag cgcggcgttg cgcatatatt gttcaaacat
      601 cggaccgcca gtttgcttga gatcataaag cttgtcaaag atattgatca tttcgttgat
      661 gacaaaagtt ttttgctccg gtttgttcgc gtcgaactcc agcatattga gcccgtacgg
      721 ccgctccata tccgccggat cgaaatagat cacatcttcc gcccgctctt ttggaacaca
      781 ctcaagcaca tgatcgacaa attcgccatg aggatcgatc acacacacgc cttttccatt
      841 tttgatgtcc tgcttcacca tttcctccat catggttgat tttccggttc cggtctgacc
      901 gacaacatag aagtggcggc gccggtcatc ctccgcatga cggatcaccg tttccacacc
      961 ccgatagtca ttgaagccga gcgtgatgcc atctttcgaa atattcgagg gcggggcggc
     1021 cgtacgagcc ttgagccagg cgatcttggg cgtctcggtc gagaaaagcg gaaagtgaaa
     1081 aacgctggcc acttcctccg aagacaggat gaaactttga tcgtcgcgaa aattgcgaaa
     1141 gataaaatca aaagcgatat ttttcggacg ccgtttgctt tcccgcagaa ctttgaaact
     1201 gttcaaaccc ggattttcgt attgtataaa gccattttcg agctcggaaa gaatcacttc
     1261 cgagcgctcc tgagtgagcg ccgaagccac cagacggata ttcacgctga aaccggattt
     1321 tccggccttg ccttcgattt ttttcaccac ttcctgctct tccggcgtga gctgaatgac
     1381 atctttgttt tgcttcagaa tatcttcttg cttgtctttt ttctgatgaa agacgcttag
     1441 cagtccgcgg ccgacctggc gcgcgatcgt atccctttta acatctgaaa gttgccggcc
     1501 ctgctgcatt ttgtgggcga caagttttcc tttttgccgc cagccgcttc gtgccggttt
     1561 gatgagaagc tggattgccg ccccttcttt tttctcgtca aactttccga gcacgttcgt
     1621 gatgttgttg agaggatcgg cttccagtgc ctgatatgtc cggatcggaa aatgatgttt
     1681 gtccttgagc gtcaggcttg aggcagccgt gaagctgccg ggagaaaaaa tattgtagtc
     1741 cttcactctt tcgatcgagg ctttgggata gaagctgtgg atctgtcgtt cgacaatatt
     1801 gatgaatttt ttcggcgccc cgacataaaa tgaaatttcg tcattgtcgg caggattggc
     1861 catttcgagc gccacgaccg cgtctccgta tatgatccga tgccaaatgt ttgatttttc
     1921 tttgatgttc cccaggctcg cgaaaagatg ttccatcacc gagatcaatt ctttttccga
     1981 tttgggatgc tgctgctcgc cgccctgcgc ttcccgcttc atcggccgac tcactttcac
     2041 cacgtcaaga tccatgttca atgatcgcgt gatgatccct cgaaaaccca caaagctttt
     2101 gaataccaaa agagcacccg acacaaagca aacaagcgcg gcaagcccgg caagagggta
     2161 taggatcaag tttaagctat aggcgtccat ctattttttt atttcttttt attttcagaa
     2221 gattacctaa aaaataattt tctaatttaa gaattttcaa ttttcaaaca atgactcaat
     2281 ttcttaattt caaaatgttt ctgaaatttc ggtcattcga aaattgtttg aaaattttgg
     2341 aattttaaaa ttttataatt cttttaacaa tcctttctgc atcaattgtg ctcggagctc
     2401 gtcttccatc agccggtcat ggatttcatc gagagtatag ttgtcggccg ccgccggatc
     2461 ctttcctttg tccatatgtt gcgccacctg gatcgcgtgt tccggtcctt tttgaatggc
     2521 aatttcgacc aatttttcca cctttttctc aaattcttca attccggaca cggactccgc
     2581 atcactcgca atgtttgcgg caggagtcgc ttgctgcgtt ccaatagtct gcctggctgt
     2641 ctcaattttt tcggaaactt tttcctcggg cgattccgcg attgtttcaa cttggacttc
     2701 ttttgatact tccggcgtcg gaatagtttc cttttttcca gcgtcaatat tcgcttctct
     2761 cagctttttc gccagatcca gatctttttg ctcgatcggc tcttgatagt ttgattgtgc
     2821 cataatagta aatttaaaat gcaaatatca aaatgtaaaa ttacaattca aaattaaaaa
     2881 tatattcatg caaaagtttc taacattcaa aaaactttac attttacact gtcattttca
     2941 attttgattt ttcaattttg aattatttta tactcccact ttcgccagct cttcttcctt
     3001 gatctttccc aaatcgccag ttgttttttt catctcctcc gtgaaatctt caacgatttt
     3061 tttgacgaat ccgtcgtagt cttcgatttt cgtgcgcaaa tacgcttcaa ctttttccgg
     3121 ttccgcctct tcagtgtcga gcatcttggc cagttcctcg cgatccgctt ccccaagttt
     3181 ttcaaaagtc tcgagatata tttttttgag aagcacttcc gtcattttga cgagaatttc
     3241 ttcctgcttc tccttgggaa gatcctgaag tcccaattct tcaatgatcg actgctggag
     3301 gattgtgtct tgtggcatat gtgtttttta atatttatct tcttataaaa ctgatttatt
     3361 aattaaatac aatatctttg gattcttcac tgcttgtttc aaacaatcgc cgattgtttc
     3421 actgccgctc gtgttatatc ttcccagtat aaggcgcaaa tatttcaatt tatttaaatt
     3481 aatattcatt tttccgactg catccgcgtc ttgaacatcc tgagttatat agttttccgt
     3541 cgcaatcagt ttttcccacg cattcatcgg tttatcaaga atattggatc gaatgctaat
     3601 gcctgactca tgaaaaattt tttctattcc ttcttttgtt gaagaaaata actgatcgtt
     3661 ggccgcttca tttacaccct ctagtcgagc tgtttcctga gcgttaaaaa ctctcagatc
     3721 tttttgatac ttatccgcca aggcaaattc tttcttcgca tttatttgtt ccggagtatc
     3781 agcatcatat tgcatcgttt ttcctgcgcc tctcgccggc tgatgctctg ccacgcctgt
     3841 tccatgtttc acggttccat gcgacaaatg tggcttgttc aaggtatttt cgtgcaccgc
     3901 accagccgct ccgccgtgtg aaacatcagg cgtgaccgtc ggtcgcatag tcgccatttc
     3961 ttccgccgcg ctaccatgca aaacatccga gccaaaagct tttccgtgaa aatcatccag
     4021 agatatttct tctgtcgttg gattatatat tatcttgaag gtgtccccag ctcggagttt
     4081 cgttcgcgca atttgatcaa gatcctcata actcattccc ttccgatagg caaattcatc
     4141 aagcatctgc ttggtcattc ggtgcatatc tttggtgaaa tttggattgt tgggatcgag
     4201 gccataggct gatggattcg ctttgatatt gttttcgata cttccccaca tattcccgcc
     4261 cgctttgatt ttcacggatg caatatcacg aatgtcagca acatgcccat ctccttcagc
     4321 tccatatccg atcaaagaac ttttttcctc cggattgacg gcctttgctt catgtccttc
     4381 tccttctgtt ccataaagtc ctgatccata ctttccttcc ggatgcgcgt ccacgagatc
     4441 atgcccctca ccttcagaac cgtataatcc aagtccatat tttccttccg gataagcctg
     4501 aacaaattca tgaccacttg cttcggagcc ataaccaacc aatccttcat gagcctgcgt
     4561 tgcgattgtg ccgcctcctg caggcgcaag ttccgcagca tatgcttgat cgggagagag
     4621 aatacgctct gcaaaatgct ttacgttgcc aaagacaccg gatgctttgt cctttatgaa
     4681 tcctcccaag ccagttccgt tgattatttg cgccattgct ccgcttccca caaatatacc
     4741 agcaaacgta gcaactcttt ttcgcgtgag atccttgctt tccatctttc caatccgcgt
     4801 atcaatcgtc ccaatctccc catcaatttt tgcgttgagc gctttcatcc aatcatcctg
     4861 gctcaatatc ttcccggcct cctcgagagc cgccttgttt tctcgtttgg ctgacactgt
     4921 gtccatcaat tttttgagtc ccacaccggt tgctccgccg ctgacaattc tctgtcccac
     4981 cttcgcagtt gctgccaacc cgacaaatat tccgcccaga tatgcgccag aaacaattcc
     5041 tcctgcgaga agcgcgcctg acacagccat tttttttcca aagcttaagc cccgccattt
     5101 gtccaccact ttcatatacc cgtccataac tttatccggc cattcacctc tctctttcct
     5161 catcttcgcg ctttcatctg tctttgcttc caaaaacaag gttgcctcac caacagtgaa
     5221 ttcggcataa agttgagctg cttgctcctt tgtcacagct ccaagcgatt ctctttttgc
     5281 gtccagatat cgtttcaaag cttgcttata ttcatttttc ttttcttcaa caccagaatc
     5341 tttgatttct ttcgagttga aaccaagaac ttttttcaat ttgttccaac gtcccgacat
     5401 atccacatca tatcgagcag ctaacattct ttttgcttcc aagtcggcct tcagcgcctc
     5461 caagctattc atcgcttcaa actccatttt tctcgcctct tccgcgttct ctgctccttt
     5521 catccttgcg tcttcaatgc tcacattatc aatctgcctt tgattttctt tcgcttgttt
     5581 tttcagggcg tcctccatcg gtgtcttgtc cggcagagtg ccggttttgc tccccgaatc
     5641 cggttctagc atttgctcgc ccgctttttt catatttttg aaagctttct ttgccgtttc
     5701 catgtcccag ttctgcataa atttttgggc ttcgtcattc cattcacctt ttccggaaag
     5761 aattttcttg atattttcct tcaaaaagat attttcatcc aaaccgtatt ttttggccca
     5821 atatttctgc ctttcaggat cattgctcat tcgatgctgt tggatatagt atgccgtatc
     5881 ttggattgcc ctcagctctt cgggagaaaa ttccggctct ttttcttttt tgtccgctgt
     5941 ccccgtcttt cgagccttca cttcctcggc ttctcttgca tcaagatcca cttccgctcc
     6001 cataaaacca gctcctgtcg gaaaagaatc tttcggcaac attcccgcct ctttcagttc
     6061 ttcgcggtct tttttgagct gttcctcaat gttgagttct ggtatttttt ccatattttt
     6121 attttttagt atcaaagtta aatttttcta catatttcat cacatgctca accgctctcg
     6181 gtatttcttc ttctgaaaat aactttttca tctctgaatc actactcatc acactattca
     6241 ttttttcctt tatttgctca aatatcgcct tcttttttgt ttcttcatct ccgaattccg
     6301 atccccactt ctctgattct ttgacccact ctttgaaacg tttgctggtc aaaccaatcc
     6361 actgaagcaa ttctgacgat tgatttggag taaattctgt ctcttttttg tctttattgt
     6421 tcatactctt tatttttttc tccggaaaac caactggcag cttcagctca agataatttc
     6481 ccaacagctc cgctttttcc ggcgcaattt ttctgttcaa tccccaccgc tttctttcgt
     6541 cgatataaaa ttgccgaatg gcttcgaccc agttttcatc aattctcact tttctcctgt
     6601 ccgccttctc cttttctccg taaaaatacg aaggatagtc atcaatcatc ggatcgataa
     6661 atcttttctc agccggatca aattcttttt ctatcttagt ctcgacttca gccaattttg
     6721 cttctctggt cttttccagc gtctcgacca cagctcccag catctcatta tcgtcacctg
     6781 accagtattt caaatcatcc ttgggataat atgacttttt attttccgct ttcaccgatt
     6841 cgggttggga ttcacttttt ttctcaacag gcttcggaat gttcggaccg aaatggatac
     6901 tactcgcatc aaccggtctc tttgaactta ggtctataat atcagctttt ttatctccct
     6961 tttctttacg cgttgaaacc ttcggcaaag ccagttctcc gctttcaaga atttgctcca
     7021 tcttccatcc ctttcctgca tttctcccga ctcctgattg gcgatgtgga tttccttttc
     7081 ccattttaaa atttttttaa ctcaaacaaa actggcgctt tgaatacgct tcggctttcc
     7141 caaattttct ttttatctta ctcaaattat accacaaaaa caaacttcac agaatggctg
     7201 tggataactt ttcttgattg ttgtataatg tgggcgtaag cactcttgag ataaataaaa
     7261 aaataaacaa aatctatgct cgaaaagtac caatattata tcgtcgccgg catacttgga
     7321 atcatgctgg tcctttccat cagcgtcgcc ctcggggatg ccgccatcat ggacgaagtg
     7381 gcccatattc cgtccggata ttcatatatc cactttcacg actatcgcct caaccccgaa
     7441 catccgccgc ttctcaagga cctcgccgga attcccctgc agtttttgaa agtcactttc
     7501 cccacagaca aaaactattg gacaaccgac gccaacggcc aatgggacgc cggatcaaca
     7561 tttttgtatc tgccggggaa taataccgaa gcaatccttt tttggtcccg ttttccaatc
     7621 atcctgattg ccctggctct cggattcatg atcttcaaat gggccaaaga cttgttcggc
     7681 accaaggccg gcctattcgc gctcacgctc tatgccttcg atcccaatgt tttgggtcac
     7741 gaccactatg tgacgaccga cctcggcatc gccgcttttt ctctttttgc ttttttcgct
     7801 tttatcaaat atctcaaaaa tccgaattgg aagacgatta ctctcgccgg aatatttttg
     7861 ggcttggctg ccctcgccaa attctcttca gttttgctct ttcctatttt tggattgtct
     7921 ctgatcgtct atgccctcgc cagaaaagaa aagttgtctt tctcttttct cggctcaggg
     7981 aaaataaaga aattttgggt acaaaaattg tatctctata tcacgacttt tattgtcatt
     8041 ttcgcaattc acggcctttt gatcggcctt gtctatgaac tgaatattta taaaatgccg
     8101 acagaaaaaa tccaccagtt gatcgacact cagctttctc ccaatggaat tcagaagatt
     8161 gccgcaccat atcttcacaa aatggcagac aataccgtac ttcgccccta ttcccaatat
     8221 ctcgtcggat tcttcatggt tttcggccgc gtcgagggcg gaaatacgac ctatttcttc
     8281 ggccaagtga ccaaccagag cttccgagcc tatttcccac tggtatttct catcaaagaa
     8341 tcacttccac tcttgatttt gctgctgttt tcgattgccg ttggactgac gatactgctc
     8401 aaaaaaaacc tgccatcttt cccgaagaaa atttggacca acatcaagga ttatttcgat
     8461 ggccacatcg ccgaattttc aatgctttct ttcatcgctt tgtattggta cgtaagcatc
     8521 accggaaatc tcaatattgg cctccgccat ctcttcccga ccttgccgtt catgtatatt
     8581 ctgatcgcca aagaagtaac tatttttgga aatagggcgg caaaaaaata ccaaaaatat
     8641 ttttggacaa tcgtgcttct gcttgttgct tggcttgcgg ctgaagtcgc tttcgcttat
     8701 cccaactatc tcgcctattt caatgaaaca gtcggcggac caaaaaatgg ctacaaatat
     8761 gtgacggatt caaatgtgga ttggggacag gatctgaagc ggctggaaaa atttgtttcg
     8821 gacaacaata ttcaaaaaat ccgcgtcgat tatttcggcg gcggagatgt ggtacattat
     8881 ctcggagaca aggccattat gtggcactcc gatttgggtc aagagcccgg ctggtacgct
     8941 atttcagcga ccttcctcca aaattctctc tactacaaga ttacggaagg gaaacctgat
     9001 tatgaatggc tgcgagaacg gacaccaacg gccgaaattg gcgggtcgat actgatctac
     9061 aaaatagatt aaagctattt tgtcttgtaa tatacttttc ctttcaccct caagtccaaa
     9121 tagtccagat ttttccttgt ttctccgttc aaatcttttt cgaaaagagt ttggatgatt
     9181 tttttcgctt catcaactga atattcagaa ctaatcttaa gagtccatcc ttcgttggtc
     9241 acgacactga cttcctgtga ggctatcccg ggagtgtgat aactggcagc cacgtcaagt
     9301 tttagatcgt cccggatcat tgcgtttact ttttcgatat aaccgataaa atccgggctt
     9361 atttttgttt tgccgatctc caccgggatg gcgctatcgt cgaccaccac caagaaattc
     9421 ccggcccgca aatcttcttc ggttccattg gctcctccat atgccaagcc gctgctgtcc
     9481 acaagatagc aagctccccc actgcaccaa accaactccg ctttccgctc aaccactttc
     9541 acgacgatcg tatcaggaaa ttttttcccg atcgacgcaa cctccagacg attgaaattg
     9601 tttttcaccg cgctcccaat attcgaaaca ctcaccaaaa aaaagttttt tttcggcaaa
     9661 tacttcaaat atttcccttc gagtgatctt tccacgactt ttgatatttc cacgctcgaa
     9721 atatcctgat tcccttggat atttatttta ttaacatcca aaaaagtcga aaagaaaagc
     9781 aaatagacgc aaatcccgag aaaaacaaca agcagcagcc aaaaaataaa gcgagaaaaa
     9841 atcccgcccc tttgggacgt ttcttttttt gatgctccgg gatatgcttt cgtatttttt
     9901 ctctgccagg atttttggac aattttgttt ttcttttttt tggaaaaaat aggcatatat
     9961 ttcctaaagt tcgactttag gcgctcctaa agtcgaactt taggaaaccg aagctataag
    10021 atttctttta caattttcgc gatcatccag gcatacacgt tgtgccggcc atattttttg
    10081 atctcgacgg attcatgctt cccttggaag gaaaaatcga atgtttgtcc ctggacagtt
    10141 gttttgaggt tgcttgcctg cgctttcgcg ccatcagaaa aaccatagct gatcttttcc
    10201 gatggcgaaa aattggccag ttttttcacc cgttcatcat caaaattcaa aatcaatttt
    10261 ccgcttgacg gcattttttt caccaaaatt ttcatttctt caaaaatatc atccagattc
    10321 gattccgcat ccgagtagct gccttcaata ttggtgacga ccacccaaga gggacgcacc
    10381 atttcaagca agtatgccat atcgcctttt ttctccactc ccagctccag aaccagtttt
    10441 gagggaaatt tccgggaaaa taaagctttc atcttggcct gaaccaagat cttccaccac
    10501 ttccaaaatg aagattctcc tgattcgaga tacaaaatcg ccaatggcag gccaatt
//
LOCUS       DATKGS010000080         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_9262, whole genome shotgun sequence.
ACCESSION   DATKGS010000080 DATKGS010000000
VERSION     DATKGS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9262"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VMQ48_02000"
     CDS             complement(<1..244)
                     /locus_tag="VMQ48_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HUD08846.1"
                     /translation="MIQAETKLKVADNSGAKTIECFKVLGGTRRRYAQLGDVVVASVK
                     SAEPRAAIKKKAIVRAVIVRQRKPFRRKDGSYIRFDE"
     gene            complement(266..550)
                     /gene="rpsQ"
                     /locus_tag="VMQ48_02005"
     CDS             complement(266..550)
                     /gene="rpsQ"
                     /locus_tag="VMQ48_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HUD08847.1"
                     /translation="MNNSDNSNIASARGGSASGGKKFLEGVVVSDKMDKTAVVAVNSF
                     KAHPKYLKRYLSTKKYKAHDPENKCKIGDKVKMAETKPMSKGKKWEIVFE"
     gene            complement(547..747)
                     /gene="rpmC"
                     /locus_tag="VMQ48_02010"
     CDS             complement(547..747)
                     /gene="rpmC"
                     /locus_tag="VMQ48_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HUD08848.1"
                     /translation="MKIKEIREKSENELKKDLVERRNKLAKMRFDISAKQVKNHRDIR
                     ITKKDIARILTVLNISKVKTIL"
     gene            complement(785..1192)
                     /gene="rplP"
                     /locus_tag="VMQ48_02015"
     CDS             complement(785..1192)
                     /gene="rplP"
                     /locus_tag="VMQ48_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HUD08849.1"
                     /translation="MLMPKKVKHRKIQRGGKIRGNAKRGTEVSFGSIGLKSLEAGLVS
                     ARQIEAARRAMTRYVQRGGKIWIRIFPDKPMTKKGAETPMGKGKGSVDHFVAVVKPGR
                     IIFEMDGVNHTIARQAMKLAADKLNVKTRIEER"
     gene            complement(1219..1857)
                     /gene="rpsC"
                     /locus_tag="VMQ48_02020"
     CDS             complement(1219..1857)
                     /gene="rpsC"
                     /locus_tag="VMQ48_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HUD08850.1"
                     /translation="MGHKVNPQGVRLGITSDWKSRWFSKKDFPSQLKEDVKIRERIFK
                     QLKNAAVGGVEVERSVGSIKIIIKTARPGVIIGRGGTGVEDIKKMIKDEFFKQKKLNL
                     KVEVEEIKNMEENAMVIAQSVAEQLEKRMPFRRVLKTTLERMMENKRINGIKIEISGR
                     LGGADMSRREWSAKGNLPLHTLRADIDFAKVTARTTFGVIGVKVWVNRGEKF"
     gene            complement(1862..>2045)
                     /locus_tag="VMQ48_02025"
     CDS             complement(1862..>2045)
                     /locus_tag="VMQ48_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08851.1"
                     /translation="KKIEPEEKIGKEELKPSSSAGREEKKEVIKEKIEKEFVDEKKKA
                     AESKGFLKRVFRRKSM"
BASE COUNT          428 a          562 c          350 g          705 t
ORIGIN      
        1 tttcatcaaa gcggatatac gacccgtctt ttcttctgaa aggttttctt tgtctcacaa
       61 tcaccgccct cacgatggct ttcttcttga tcgcggcgcg tggctcagcc gatttcaccg
      121 aagcaacaac cacatccccg agttgggcat atctccggcg cgtcccgccg agcactttga
      181 aacattcaat ggtttttgct ccgctgttgt cggctacttt caattttgtc tcggcttgaa
      241 tcatatactt gtcttaaaat tccatttatt caaatactat ttcccatttt ttccccttgc
      301 tcatcggctt ggtttcagcc atcttcactt tgtcgccaat tttacacttg ttctcgggat
      361 catgggcttt gtactttttc gtggacaaat atctcttcaa atattttgga tgagctttga
      421 aagaattcac cgccaccacg gccgtcttgt ccatcttgtc gctcacaacc acgccttcca
      481 aaaatttttt tccgccagag gcggatccgc ctctggcgga cgctatattg ctattatctg
      541 aattgttcat aaaattgttt taacttttga aatattcaaa acggttaata ttcttgcaat
      601 atcttttttc gtaattctga tatcccgatg attcttcact tgctttgccg aaatatcaaa
      661 ccgcatcttg gccagtttgt ttctgcgctc aaccaaatct tttttgagct cgttttcact
      721 tttttctcta atctctttaa tcttcatatc tttaatcaaa tttattcgaa ttcattcaaa
      781 tatcctatct ctcctcgatt cttgtcttca cgttcaattt gtctgcggca agtttcatcg
      841 cctgcctggc aatcgtatgg ttcactccgt ccatttcaaa tatgattctt cccggcttca
      901 caaccgccac aaaatggtca actgacccct tgcccttgcc catcggggtt tccgcacctt
      961 ttttggtcat cggcttgtca ggaaaaatgc ggatccaaat ctttccacca cgctggacat
     1021 agcgggtcat cgcgcgccgc gccgcttcga tctgcctggc agaaacaaga ccggcttcca
     1081 aagatttgag accgatgctt ccaaaactca cttccgttcc gcgcttcgcg tttccacgga
     1141 tcttgccgcc tcgttggatc tttctgtgtt ttactttctt gggcatcaac ataataatcg
     1201 aaaattcaat gagtcaaatt aaaatttctc tcctcgattc acccagactt tcacgccgat
     1261 cactccgaat gttgttctgg ctgtgacttt ggcaaaatca atgtcggcgc gcagtgtgtg
     1321 caagggcaag ttccccttgg ccgaccattc gcgacgggac atatctgctc cgcccaacct
     1381 tccggatatc tcaatcttga ttccgttgat tctcttgttt tccatcatac gttcaagcgt
     1441 cgtcttgaga acgcgacgga aaggcattct tttttccagc tgttccgcaa cgctctgggc
     1501 aatcaccata gcattttctt ccatattctt gatctcttcc acttccacct tgagattcag
     1561 ttttttctgc ttgaaaaatt cgtcttttat cattttcttg atgtcttcca cgcctgtgcc
     1621 accccggcca ataatgactc ccggtcgcgc agttttgatg ataatcttga tcgaaccgac
     1681 agatctttcc acttcaacac cgccaaccgc cgcatttttg agctgcttga aaattcgctc
     1741 acggatcttg acgtcttctt tgagctggct aggaaaatct ttcttcgaaa accatcgcga
     1801 tttccagtcg cttgtgatcc ctaaacgcac gccttgtgga ttgactttat gccccataaa
     1861 attacattga ttttcttctg aatactcttt tcagaaaacc cttcgattcg gccgcttttt
     1921 tcttctcatc aacaaattct ttctcgattt tttctttgat cacttccttc ttttcttccc
     1981 tgcccgccga agaggagggt ttcaattcct cttttccgat tttttcttcc ggctctattt
     2041 tcttg
//
LOCUS       DATKGS010000081         2900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_9379, whole genome shotgun sequence.
ACCESSION   DATKGS010000081 DATKGS010000000
VERSION     DATKGS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9379"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VMQ48_02030"
     CDS             complement(<1..1307)
                     /locus_tag="VMQ48_02030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08852.1"
                     /translation="MKERIITILIFFVIVASSFYFGFHRLASFSGVDEPYWSYGRVPN
                     FWNAVQKHKWKNTNISDKPGVPLAIVSGAGLPFIGENPKSLKSLRYNPKTPEQIQEIR
                     DIYFKLRLPVFLFTLAMLPVFYWLIKKLLGKNVARFSLLFIGLSPVLLGISLIINSDA
                     LLWILTALSTLSLFVFLKDKQRKFLLLSGFFLGLSVITKYVANILLVYFFLVFFLEYI
                     FHAHKKVAIGEYLEKALANYVILVLATMVTAFVFFPATWVKISTLLNATIGNKVFSST
                     WPLFVGLIALVAADTFLLESKFSKLVFGFLAKYNGVIAKFFAGLFLAFAAFVFLHVYG
                     HVHIIDIQALISQPKGLGQGNVLQKYGGAISSDLYSLLFSVSPLILLFILFAVINFFR
                     KKELKRDTITAVYIIIFIFIFYLGNAVNSVIATVRYQIIVYPL"
     gene            complement(1417..>2900)
                     /locus_tag="VMQ48_02035"
     CDS             complement(1417..>2900)
                     /locus_tag="VMQ48_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08853.1"
                     /translation="LIKRLLGKQIARASIIFIGLSPVLLGISLMINADAVLWSLIPLA
                     LLSFLIYQKENNRNFLYLSGFLLGLALIDKYIANILFLFFFGLIFLKYIFEDDARGAE
                     KYFKKAFADFGLLILVSSVTIFAFFPSVWVKPKILLDVTIYSKAFYKTWRIFFALLIL
                     AAADTYLLKSKIIGLICNPIRARKNWLRGAVYAIALALMAFVLLNTYSGMKFFDFQPV
                     FNFPTLELKQINARFGEPLIIMLSSFYPLLFSLIPVVAILFVFAVVRSFARRMEKNES
                     LQVFYLLMFILFYYLGSAASGVSPTPRYQIVLYPVASIIAAIGLSHFLDMKSLKKYFS
                     SARSRAAVLLILFAVSIWSLFSIKPFYLSYTSDLLPKQDILDLRNMGDGSWEAAQYLN
                     SLPDAKNIKVWADEGGVCEAFMGACSDTLKPNQLNTNFDYFVVSAGREAKSIRMAQTR
                     LTKLNGAIEVGSLYSETNPCKFKLNVGGRSGNFVKVMKADSLP"
BASE COUNT          920 a          644 c          693 g          643 t
ORIGIN      
        1 atcagcgggt acactatgat ctggtatctc accgtcgcaa tcaccgagtt taccgcgttg
       61 ccgagataaa agataaagat aaaaatgatg atatataccg ctgtgattgt atcgcgcttc
      121 agctcttttt tcctgaaaaa attgataact gcgaacaaaa taaacaggag aatgagcgga
      181 gaaacgctga aaagcaagct atagagatcg gaagaaatcg ccccgccgta tttttgcagc
      241 acgttgccct gcccgagtcc tttgggctgc gaaataagcg cctgtatgtc gataatatga
      301 acatgcccat aaacgtgcaa aaaaacgaaa gcggcgaaag ccagaaacaa accagcgaaa
      361 aatttggcga tcaccccgtt atatttcgcc aaaaaaccga atactaattt cgaaaacttg
      421 gattcgagca agaatgtgtc tgcggcaacc aaagcaatga gaccgacaaa aagcggccac
      481 gtggatgaga aaactttgtt tccgatcgtg gcgttcagaa gcgtcgaaat cttgacccac
      541 gtcgccggga agaaaacaaa agccgtcacc atagtcgcca ggacaagaat gacataattg
      601 gcaagcgctt tttccaaata ttccccgatg gcaacctttt tgtgggcatg aaaaatatat
      661 tccaaaaaga aaaccaagaa aaaatagacc aggagaatat tggcgacata cttggtgatg
      721 acggaaagac caagaaaaaa tccggagaga agcaggaatt ttctttgttt atctttcaaa
      781 aaaacaaaca ggctcaaagt ggaaagcgcg gtcaggatcc aaagaagagc gtccgaattg
      841 atgatgaggg aaattcccag caggaccggc gaaaggccta tgaaaagaag cgagaacctg
      901 gccacgtttt ttcccagcag cttcttgatg agccaataga aaacgggaag catcgccagg
      961 gtgaacaaaa aaacaggcag acgcagcttg aaatagatgt cccgtatctc ttggatctgc
     1021 tctggagttt tcggattgta gcggagcgac ttgagacttt tcggattttc cccgatgaat
     1081 ggcaagcccg cgccggaaac gatcgccaga ggcacgcctg gcttgtcgga tatattcgta
     1141 tttttccact tgtgcttttg aaccgcattc caaaaatttg gcactcggcc atatgaccaa
     1201 tatggctcgt ccaccccgga aaaactcgcc agacgatgaa acccgaaata gaagctggaa
     1261 gcgacaataa caaaaaagat caggattgtg ataattcttt ctttcatagg attgttttgc
     1321 tccttaatcc aataaaaact agccttaatg gcttcgaatt aggctatata attatatccc
     1381 aaaaaaacga gtttgtgaat gggaccagag aaaatctcaa ggcaagctgt cagccttcat
     1441 cactttcaca aaatttccgc tccggccgcc aacgttcagt ttgaatttgc acggatttgt
     1501 ttccgaatag aggctgccga cttcgatggc gccgttcagt ttcgtaagtc ttgtttgtgc
     1561 catacggatg gatttggctt ctcttcccgc actgacgaca aaatagtcga aattggtatt
     1621 cagctgattc ggcttgaggg tatccgaaca ggctcccatg aacgcctcgc ataccccgcc
     1681 ttcgtccgcc cataccttga tgtttttcgc gtcgggaagg ctgttgagat attgggccgc
     1741 ttcccagcta ccgtcaccca tattgcgcaa atccaggata tcttgtttcg ggagcaggtc
     1801 ggatgtatac gacagataaa agggcttgat ggaaaagagc gaccatatgg aaacggcaaa
     1861 caagatcaaa agaacggctg cacgtgaacg ggcggaggaa aaatattttt tcagactttt
     1921 catgtccaga aaatgactaa gtccgatagc agcgatgatg gaagcaacgg gatagaggac
     1981 gatctgatag cggggagtcg gactcactcc gctggcagcg gatccgaggt agtaaaaaag
     2041 aatgaacatg agcaaataga agacctgcag cgactcattc ttttccatcc ggcgcgcgaa
     2101 ggatcgcact acggcaaaca cgaaaagaat ggccacgacg gggatgagac tgaagaggag
     2161 cggataaaag gaagatagca tgatgatgag aggttcgcca aaacgcgcgt ttatctgctt
     2221 gagctcaagc gtcggaaagt tgaagacggg ctggaaatca aaaaatttca ttcccgaata
     2281 tgtgttcaag agaacaaaag ccatcaaagc gagcgcgatg gcatatacgg ctccacgaag
     2341 ccaatttttc cgcgcccgga ttggattgca tatcaggccg atgattttgc ttttcagaag
     2401 ataggtatcg gcagcggcca aaatcaagag cgcgaaaaaa atgcgccaag tcttgtaaaa
     2461 tgccttgctg tatatggtca cgtcgagcaa aattttcggc ttcacccaga cggacgggaa
     2521 aaaagcaaag attgtcacgg acgaaaccag gatcaaaagt ccgaaatcgg caaaggcttt
     2581 tttgaaatac ttttcggcgc cgcgcgcatc atcctcaaaa atatatttca aaaaaatgag
     2641 tccgaaaaag aaaagaaaga ggatattcgc aatatatttg tcaatcaaag ccagtcccag
     2701 caaaaatccg cttaagtata ggaaatttcg gttgttttct ttttgatata tcaggaaact
     2761 caaaagcgcg agagggatca agctccacaa aacggcatcc gcgttgatca tcaatgaaat
     2821 cccgagaagc accggggaca gcccgatgaa aatgatcgac gcccgggcga tctgcttgcc
     2881 caacagtctc ttgatcagcc
//
LOCUS       DATKGS010000082         4083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_95616, whole genome shotgun sequence.
ACCESSION   DATKGS010000082 DATKGS010000000
VERSION     DATKGS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95616"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..726)
                     /locus_tag="VMQ48_02040"
     CDS             complement(226..726)
                     /locus_tag="VMQ48_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HUD08854.1"
                     /translation="MKIILMMAMTADGKIAKTSDHFPDWTSKEDKKYFFETTKKHGAV
                     LMGEKTFKTFPKPLPDRLNVVFTLEKNPKKQENVKWVSGDPEKVLDDLENMGYKSAIL
                     GGGAFLNSQFLKKKLIDEIWLTIEPKIFGDGLGIFSGDFNQDLKLISVEKINENSVVV
                     KYEVVK"
     gene            complement(736..2406)
                     /locus_tag="VMQ48_02045"
     CDS             complement(736..2406)
                     /locus_tag="VMQ48_02045"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006586706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HUD08855.1"
                     /translation="MLSDIEIAQKAKMKPVKDIAGLVGIGENDLELYGDYKAKLKPEL
                     WEKIKSNPDGKLILVTAINPTPAGEGKTTISIGLAQAFNKLGKYAVLALREPSLGPCM
                     GIKGGAAGGGYAQVMPMEDINLHFTGDIHAVTAANNLLASLIDNHIYFGNKLEIDLQN
                     IFWKRCMDLNDRALRKVELKIEKKSGNYSREDGFNISVASEIMAILCLSENMADLKKR
                     LGNIVVGLTSAGKPVYARELKAEGSLAALLKDALKPNLVQTLEHTPALIHGGPFANIA
                     HGCNSILATKYALKLGDFAITEAGFGADLGAEKFFDIKCRLAGLKPDAVVIVATIRSL
                     KYNGGAEKENLTEENIDALSRGMANLEKHLENLKKFSVPAVVAINIFPTDTEKEIELV
                     EEKAGKLGAKFARSYVWEKGGEGGIELANAILETIENEKSDFAPLYDEKKSITEKIET
                     IARKIYGAGSVSFSEKAKERISMLEAENLDKVPICIAKTQYSLSDDPRLLGRPEGFEF
                     NIRELRLSNGAGFIVAIAGDMMTMPGLPKVPAAEKIDVDDKGRISGLF"
     gene            complement(2426..3034)
                     /locus_tag="VMQ48_02050"
     CDS             complement(2426..3034)
                     /locus_tag="VMQ48_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HUD08856.1"
                     /translation="MEKHFKDERTLVVIKPDGVQRSLVGEIIKRFEQVGLKLLAVKML
                     VPTEEHIDKQYTLDPEWRRVTGEKTIKGYQDKGLEPPSMDPLAITEIILSNLKRYMTS
                     GPVVAMVWQGAHAVKIVRKLTGGTEPLTSDVGTIRGDYVLDSYQMGDTDKRAVRNLLH
                     ASGSPEEAEMEIKHWFSDKEINDYRLVQEGIIYDVNLDGILE"
     gene            complement(3589..>4083)
                     /locus_tag="VMQ48_02055"
     CDS             complement(3589..>4083)
                     /locus_tag="VMQ48_02055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HUD08857.1"
                     /translation="EAGVQYFGDTGDDQSPALVKTISGTKIGFVNYNYSIAGSFERAV
                     EDIKNIRNKSDIVVVCPHWGTEFKIGDPGQKIRAEAYKLIDAGADLIVGGHPHVVQDS
                     EDYKNKKIYYSLGNFIFDQYFQKETMEGLGVEVTINPDRAIQFNELKFEMTKRGQTIL
                     KVEP"
BASE COUNT          987 a         1063 c          811 g         1222 t
ORIGIN      
        1 ggggggcaca agtttcggct gttttcaagt cacatggagc accttgcttc cccgaaaggc
       61 agaaataata gggacagcac ccaccttctc tcactcccgc tgcttctctt cttcgacctt
      121 cctttaactg atcatctagt ggacaatcag tgggaaaatc gaacttcagc cacatttccg
      181 gattatattt tcttgcttcc acgcctacct cctttgaaca tggatttatt tcaccacttc
      241 atacttcact accaccgaat tttcgttgat cttttcgacg ctgatcaact tcaaatcctg
      301 attgaaatca ccgctgaaaa ttccaaggcc gtctccgaaa attttcggct cgatggtgag
      361 ccagatctcg tcaatcagtt tctttttcaa aaattgtgag ttcagaaacg cgccgccgcc
      421 cagaatagcc gatttgtatc ccatattttc caaatcatcc aagacttttt ccggatcacc
      481 cgaaacccat ttcacatttt cttgtttctt gggatttttt tcgagcgtga agacgacatt
      541 cagccggtcc ggcaatggct ttggaaaagt cttgaatgtt ttctcgccca tcagcaccgc
      601 gccgtgtttt ttcgtcgttt caaaaaaata ttttttatct tctttcgaag tccaatccgg
      661 aaaatgatcg ctcgtcttcg cgatctttcc atccgccgtc atcgccatca tgagaataat
      721 tttcataaat tttttctaaa aaagtccgct aattcttccc ttgtcatcca cgtcgatctt
      781 ttccgccgcc ggaactttgg gaagcccggg catcgtcatc atatctcccg cgatcgccac
      841 aatgaacccg gcaccgttcg aaagccgcag ttcacgaata ttaaattcaa atccctccgg
      901 ccgaccgagc aatcttggat catccgaaag agaatattgg gttttggcga tgcaaatcgg
      961 cactttgtcg agattttccg cctcaagcat tgaaattcgc tcttttgctt tttcagaaaa
     1021 tgaaaccgat cccgccccat atatttttct cgcgatggtt tcaatttttt ccgtgataga
     1081 ttttttctcg tcatagagag gagcaaaatc ggatttttca ttttcaatgg tctcaagtat
     1141 ggcgttggca agttcaattc ctccctcccc gcctttttcc caaacgtatg aacgggcaaa
     1201 ctttgctccc agtttccccg ctttttcttc cactaattct atttcttttt ccgtgtcggt
     1261 cgggaaaata ttgatcgcca ctacggccgg cacgctaaac tttttcaaat tctcaagatg
     1321 tttttccaga ttggccattc ctcttgaaag cgcgtcgata ttttcctcgg tgagattttc
     1381 tttttccgct cccccattat atttgagcga tcggatcgtc gccacaatca ccaccgcatc
     1441 cggcttcagc cccgccaatc ggcattttat gtcaaaaaac ttttccgccc caagatccgc
     1501 tccgaatccg gcttccgtga tggcaaaatc accaagcttc aaggcgtatt tggttgcaag
     1561 gatgctattg cagccgtgcg caatgttcgc gaacggtccg ccatgaatga gagcaggcgt
     1621 atgttccaag gtctgaacca aattcggctt aagcgcatct ttcaagagtg cggcgagaga
     1681 cccttcggct ttgagttccc gggcataaac cggctttccg gcagaagtga gaccaacaac
     1741 aatatttccc aatctttttt tgaggtcggc catattttcc gaaaggcaca aaattgccat
     1801 gatttccgac gcgacggaaa tattgaatcc gtcttcgcgg gaatagttgc ctgatttttt
     1861 ttcaatcttg agctcgactt ttcgcaaagc gcggtcattg agatccatgc aacgcttcca
     1921 aaatatattt tgaagatcaa tttccagttt gtttccgaaa tatatatggt tgtcgatgag
     1981 agacgccaaa aggttgttcg ccgccgtgac cgcgtgaatg tcgcccgtga agtgcaaatt
     2041 gatatcctcc atcggcatca cttgcgcgta tccgccgccc gccgctccgc ctttgatgcc
     2101 catgcacggc ccgagggatg gctcgcgcaa ggccagcacg gcatatttgc caagtttatt
     2161 gaaagcctgc gcgaggccga tagaaattgt cgtttttcct tcgcccgccg gagtcggatt
     2221 gatagccgtc accaggatca atttcccgtc cggattgctt tttatttttt cccagagttc
     2281 gggcttgagt ttcgctttgt aatcgccata gagttccaga tcattttcgc caatgccaac
     2341 aagacccgcg atatccttta caggcttcat tttggctttt tgcgctattt caatgtcgga
     2401 tagcatggcg ctttgcggaa aaaatttatt ccaatattcc gtccaagttc acgtcgtata
     2461 tgatgccttc ctgaacaagg cgatagtcat tgatttcctt gtctgaaaac caatgtttga
     2521 tttccatttc ggcttcttcg ggagatccgg aagcatgcaa aagattgcgg acagcacgct
     2581 tatcagtatc gcccatttgg tatgaatcaa gaacatagtc cccgcgaatt gttccgacat
     2641 cagaagttag aggttccgtt ccgcccgtaa gttttctcac aattttcacc gcgtgcgccc
     2701 cctgccagac cattgcaacg accggaccgc tagtcatata tcttttcaaa ttgctcagaa
     2761 taatttccgt gatagcaaga ggatccatcg aaggcggctc aagtcctttg tcctgataac
     2821 ctttgatcgt cttttcgccc gtcactcgcc gccattccgg atcaagagtg tattgcttat
     2881 cgatatgttc ttccgtcggc acaagcattt tgactgccaa aagtttgagg ccgacctgct
     2941 cgaatctttt aataatttcc ccaaccagtg atctttgcac gccgtcgggc ttaatgacca
     3001 ccagcgtccg ttcgtctttg aaatgctttt ccattttttt gaaaattact tgattataaa
     3061 atggtattca ccattttgat gtcatcgctt gctcggaaat taaaacgagt tttaatttca
     3121 ctcacttcac tcgacaataa aaacattagc gaaccccgct aacagggtta taatagcaga
     3181 aaaatatttt cgaggcaaga aaaagccggc cacatctgcg agaatccgca aacgagccgg
     3241 cttcgggtct gagcgcccca ttaacgaccg tacgtataca agccgtcaac ctgactcttg
     3301 ggctgcaccc tagagttgta atcgatggtg cccacagcca gaaaggcacc gaaaatgaac
     3361 agggcaataa aaataagctt ccagcgcgcc atcttttccc ctccccggag aatttttttg
     3421 taagaactac ttattttatt gcaacgcacc gtcgcaatag tgcattttat cacgaaaaaa
     3481 cagctgtgtc aatcggctgt gaaacaactg ggaaataggg cttacacaag gcttttttgg
     3541 tttttccaag gtcaaacctt ttttaactcc gaataaattc ggagtttttt aaggttcgac
     3601 cttcaatatc gtctgccccc ttttcgtcat ctcaaatttt agctcgttga attgtatcgc
     3661 gcgatcggga ttaatcgtca cttccactcc cagtccttcc attgtttctt tttgaaaata
     3721 ctgatcgaag atgaaattgc cgagggaata gtatattttt ttgtttttat aatcctcact
     3781 gtcttgaaca acgtggggat gaccgccgac gatgagatcc gcgccggcat ctatcaattt
     3841 atacgcctcg gctctaatct tctgtccagg atcgccgatt ttgaattccg ttccccagtg
     3901 aggacaaaca actacgatat ccgacttgtt gcggatattt ttaatatcct cgaccgctct
     3961 ctcgaaactg ccggcaattg aataattata attcacaaat ccgatttttg ttccgctgat
     4021 tgtcttgaca agagccggac tctgatcgtc tcccgtgtcg ccaaaatatt gaactcccgc
     4081 ttc
//
LOCUS       DATKGS010000083         6252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_51007, whole genome shotgun sequence.
ACCESSION   DATKGS010000083 DATKGS010000000
VERSION     DATKGS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51007"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /gene="tsaD"
                     /locus_tag="VMQ48_02060"
     CDS             <1..982
                     /gene="tsaD"
                     /locus_tag="VMQ48_02060"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUD08858.1"
                     /translation="WKRDSQIKVHAPFGGVVPNLAAREHLKNLPLMLKKTFKKHKITS
                     KDIDLIAVTQGPGLIPALLIGTNSAKALAYVWKKPLVGVHHIAGHIYANFIRESSKLN
                     GIKFPILALIVSGGHTQLILMKKHMDFQIVGETLDDAAGEAFDKVARILNLGYPGGPK
                     VAVEAAKFSNFKFQISNFRLPRPMINSNNLDFSFSGLKTAVLYIVRDNPKILKNKKLK
                     SELCAEFQQAVIDVLIAKTLKAAKKYNPKSILLGGGVSANRELRKQLSEAVKNNFPNT
                     LYSIPNTLYSLDNAAMIAVAGYFQYKLGKNKNKFSNNWKNLEADAGLELK"
     gene            983..1588
                     /locus_tag="VMQ48_02065"
     CDS             983..1588
                     /locus_tag="VMQ48_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08859.1"
                     /translation="MKNIIKIIFLAIVSAALAIVVEQLIAAVASAFWQREIIFESYMH
                     FTWFLAFSAAVEEISKYWAVYFVIRKKFGLEKIKFVLASLFLGAAWGIFEIGLVLFSD
                     QKVLSAFRSGNPQVIFSFGTIVALHTLTAFLMGTFISSNSSSSKLRHFQIILFPVLLH
                     VLFNFLIIQKGNFTDFLTVVALAIGYLVGLIVLASNFKKLA"
     gene            1646..2212
                     /locus_tag="VMQ48_02070"
     CDS             1646..2212
                     /locus_tag="VMQ48_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUD08860.1"
                     /translation="MKEKKSFFERLTGTRNIPEGDSYREQSHSFGYQSQQPKPPIGGQ
                     PIQTQSFQQEEYAVEPTAPTAAPAEQPDENWGGDSEGQLTIDVYQTDAEIVIKSTIAG
                     VKPEDLDVSINNDMVTIRGDRKNDEVVEGENYYYQECYWGTFSRSVILPVEVIADKAE
                     AAMKNGILTIRLPKADTTKSKKIQVRGF"
     gene            2425..3843
                     /locus_tag="VMQ48_02075"
     CDS             2425..3843
                     /locus_tag="VMQ48_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUD08861.1"
                     /translation="MKNDLDILLVNPHLEFPPDTSGRSGHFRSREYLLNAGLLSIATY
                     LKSIGIDTVLCDLTTVDNPKRELGALVRECRPKFVGISNQSCHSYLSTMRCANIAKSI
                     DKDIKIIVGGLHASGIPKVLLRECSAVDVVIVGDGELVIADLIKGTNTSGCRRRTRTS
                     NRSIIDGLQPPNLNDLPSPDYTLYPNYRLFVPYIEESRGCNFKCAYCISPTIHRGIRF
                     WHPETIASNLTSLQNLYGREGFHTFIEANNFATSYKRTKALAELLANSNASWRVESRS
                     DTFPISLLDPLVDAGLRVLDIGLESGSPEMLLRMNKTKDPRTYLDACVKIAEKIAANG
                     KCLLKLNIMLYYGESTRSIKTTRDYLKLISSICPLSLGIGPVRMEPGSKLYRNGVQQM
                     DLSRFENTFWGKVHCYPIDLSEEISFETANKICLEIAQEFQTSKAYFEAKRHSQLRYD
                     ITYEEFMEQSEKISANERQWRI"
     gene            3989..5290
                     /locus_tag="VMQ48_02080"
     CDS             3989..5290
                     /locus_tag="VMQ48_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HUD08862.1"
                     /translation="MKRHIFFVALIFALAPFASHAEEVNLGSFSIDEIQKITGIPINF
                     HVADKTVVADKNALAGWLKIRPALRISADYSSEAENINFIPTNDPAAKIYFRFLDLQR
                     GRSHIRISTSLALDTDQLKAYLEKAKDASRVDPQNAKLSFGGGSVSAFSLSKDGYEID
                     TEKSFQDILKSLTQNPLTKDITLETNALKPDVASTDLDKYGIKELLSEGVSNFGNSPA
                     NRIHNIQVGAARFNGVLIKPQEEFSFIETLGPVDESTGYLPELVIKTDKTVPEFGGGM
                     CQVSTTMFRAALLAGLKITARTNHAYPVSYYNPQGLDATVYIPKPDLMFLNDTPNYIL
                     VQTHITGTQLFFDFYGTSDGRQTKLDGPTVLEKNPDGSMKTDLFQIVSDASGNLIRKS
                     EFKSNYKSPSLYPHPGDEKITEKPSDWSQKEWKAYKKANGV"
     gene            5346..5417
                     /locus_tag="VMQ48_02085"
     tRNA            5346..5417
                     /locus_tag="VMQ48_02085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5379..5381,aa:Arg,seq:tct)
     gene            5425..5497
                     /locus_tag="VMQ48_02090"
     tRNA            5425..5497
                     /locus_tag="VMQ48_02090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5458..5460,aa:Lys,seq:ctt)
     gene            5525..5595
                     /locus_tag="VMQ48_02095"
     tRNA            5525..5595
                     /locus_tag="VMQ48_02095"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5557..5559,aa:His,seq:gtg)
     gene            5729..6118
                     /locus_tag="VMQ48_02100"
     CDS             5729..6118
                     /locus_tag="VMQ48_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUD08863.1"
                     /translation="MEQNQTGEKTILIVEDDTFMGSLLERKFKQKNFQILRAMNVDEA
                     RKFIESGPVSLILLDIILPGTDGITFLKELKLNPQFKDIPVIITSNLGKEEEIEKGIA
                     EGATDYIVKAHTTPGEIVEKVEKILGI"
BASE COUNT         1809 a         1506 c         1335 g         1602 t
ORIGIN      
        1 gtggaaaagg gattcgcaaa tcaaagtcca cgcccctttc ggcggggtgg tgccgaatct
       61 ggccgcccgc gaacatctca aaaatctgcc gctgatgctg aaaaaaactt tcaagaaaca
      121 taaaataact tcgaaagata tcgacttgat tgccgtcacg caaggccctg gattgattcc
      181 ggccttgttg atcggcacaa attccgccaa agccttggcc tatgtttgga aaaagccgct
      241 cgtcggcgtt catcatatcg ccggacatat ctacgcgaac ttcattcgcg aaagctcaaa
      301 attaaacgga ataaaatttc ctatcttagc tctcattgtt tccggcgggc atacgcagct
      361 tattttgatg aaaaagcata tggatttcca gattgtcgga gagactttgg atgacgcggc
      421 tggggaggcc tttgataaag tagccaggat tttgaatctc ggttatcccg gtgggccgaa
      481 ggtcgcagtc gaagccgcta aattttcaaa tttcaaattt caaatttcaa attttaggtt
      541 gcctaggcca atgatcaatt cgaataatct tgatttttct ttttccggtc tcaaaacagc
      601 tgtcctgtat attgtcagag acaatcctaa aattttaaaa aataaaaagc tgaaaagtga
      661 actttgcgcc gaatttcagc aagcggtgat tgacgtactg atcgccaaaa ctctgaaagc
      721 cgccaaaaaa tataatccga agagcatttt actgggaggc ggggtatctg ctaatagaga
      781 acttcgcaaa caattgagtg aagccgtaaa aaataatttt ccaaatactt tatactctat
      841 accaaatact ttatactcat tggacaacgc cgctatgatt gccgtcgcgg gatattttca
      901 atataaattg gggaaaaata aaaataaatt ttcaaataat tggaaaaatc tggaggccga
      961 cgcaggatta gagttaaaat aaatgaaaaa tatcatcaaa ataatctttc ttgccatcgt
     1021 ttccgccgcg ctcgcgattg tcgttgagca actcatcgcc gcggtggcca gcgctttttg
     1081 gcaaagagaa atcatcttcg aatcctatat gcatttcacc tggtttctcg ctttttcagc
     1141 cgccgtcgaa gaaatttcga aatactgggc ggtatatttt gtgatccgga aaaaattcgg
     1201 tttggaaaaa ataaaatttg tccttgcttc tcttttcttg ggcgcggctt ggggaatttt
     1261 tgaaatcgga ctcgttcttt tttccgacca aaaagtcctt tccgcttttc ggtccgggaa
     1321 tccgcaagtg atcttttctt tcggaactat tgttgcgctc cataccctca ccgcgttttt
     1381 gatgggaacg ttcatatcgt caaatagcag ttcgagcaaa ttgaggcact ttcagatcat
     1441 ccttttccct gttctcttgc acgtcctttt caattttttg atcatccaaa aaggcaattt
     1501 caccgatttc ttgacagttg tcgctctcgc catcggctat ctcgtcggac tcattgtttt
     1561 ggcctccaat ttcaaaaaac ttgcctaatc gcgaaactgg ttatatacta aaaacatcaa
     1621 aaatcattag tagccaaaca aacctatgaa agaaaaaaaa tcatttttcg agaggctcac
     1681 cggaaccaga aacatcccgg agggagattc atacagggag caaagccatt cctttggcta
     1741 tcagtcccag cagcctaagc cgccgatagg aggacaacct atccaaactc aaagtttcca
     1801 gcaagaagaa tatgccgttg aaccgactgc gccgacagct gcccctgccg agcagcccga
     1861 cgagaattgg ggcggcgatt cggaaggcca gctcacgatt gatgtctatc agactgacgc
     1921 tgaaatcgtc atcaaatcga ccatcgccgg cgtgaagccc gaagatttgg acgtttcgat
     1981 caacaatgat atggtgacca tccgcggcga ccgcaaaaac gatgaggtcg ttgaaggcga
     2041 aaattattat tatcaagaat gctactgggg aaccttttcc cgctcggtca tccttccggt
     2101 cgaagtaatc gccgacaagg ccgaagccgc catgaaaaac ggcattctca ccatccgcct
     2161 ccccaaagcc gacacaacca agagcaaaaa gatccaagtg cgggggttct aagcgaaaaa
     2221 catttccatt gtttcacaag attttgcttt atacatgcag gagcagggtt gtaccctgct
     2281 ccttttgttt ccccttgctt tttccgccca atttccttac attatatgca cgaggaggcc
     2341 gagcgggagg gcttggtttt tctgttgctt taaaaattta gataaaatat caatcgcgat
     2401 ggcgatatac gggggagctt tcaaatgaaa aatgatctgg atattctgct tgtcaatccg
     2461 cacttggaat ttccgcctga tacgagcggc agaagcggcc attttcggtc gagagaatat
     2521 ttgctcaatg ccggattgct ttcgattgcg acttatctca aaagtatcgg cattgatacc
     2581 gtactgtgcg acctcacgac tgtggacaat ccaaaaagag agttgggggc tctcgtccgc
     2641 gaatgtaggc cgaaatttgt cgggatatcc aaccaatcct gccactccta tctttcaaca
     2701 atgagatgcg caaacattgc aaaatcgatc gacaaagata taaaaattat tgtcggcggt
     2761 ttgcacgcca gcggaattcc aaaagttcta ctccgagaat gttcagctgt cgatgtcgtt
     2821 atagtcggtg atggcgaact ggttattgct gatttgatta agggcacaaa tacttcggga
     2881 tgcagaagaa gaacaagaac aagcaaccga agcatcatcg atggcctaca gcctcctaat
     2941 ctcaacgatc ttcccagccc cgactatacg ctctatccca attaccggct atttgtcccg
     3001 tatatcgaag aaagccgcgg atgcaatttc aaatgcgcgt attgtatttc cccgacaatt
     3061 cataggggca taagattttg gcatcccgaa actatcgcat ccaatcttac ctctctgcaa
     3121 aatctttatg gccgcgaagg ctttcacact ttcattgaag caaataattt cgcaaccagt
     3181 tataaaagaa cgaaagcctt ggccgaactt cttgcaaaca gcaacgcttc gtggagagtt
     3241 gaatcgagat ccgatacctt cccgatatcg cttctcgatc ctctcgtgga cgcgggtctg
     3301 agagtgttgg acatcggtct tgaatcgggc tctccggaaa tgcttctgcg gatgaacaaa
     3361 accaaagacc cgagaactta tctcgacgcc tgcgtgaaga tcgccgaaaa aattgcggcc
     3421 aatggcaaat gcctgctcaa actgaatatc atgttgtatt acggcgaatc aacccgatca
     3481 ataaaaacca caagagacta tctgaaactc ataagcagca tttgtccctt gtcgctcgga
     3541 attggacccg tacgaatgga gccgggcagc aaactatacc gtaatggcgt tcagcagatg
     3601 gacttatcgc ggttcgaaaa cactttctgg ggaaaagttc actgctaccc aatcgacctc
     3661 tctgaagaaa tatcattcga aactgcgaat aaaatttgtc tcgaaattgc gcaggaattt
     3721 caaactagca aagcatattt cgaggccaaa cgacattccc agcttcgcta tgatataacc
     3781 tatgaagaat ttatggaaca atcggaaaaa atttcagcaa acgagaggca atggagaata
     3841 tgagaaatcc agttcgcctt tcgcttataa ccccgccgct gctgattgca cccaatcggc
     3901 cgcggcggct ttttttattc caaaatcatt taatctcatt ttccgcaata acttgtataa
     3961 acaacgtttt tcaagtatcc taaagacaat gaaacgccat attttttttg ttgccctgat
     4021 ttttgcgctt gcaccttttg caagccatgc cgaggaagtg aatctcggca gtttttccat
     4081 tgacgaaatc caaaaaatca caggaatccc aatcaatttc cacgtcgcgg acaaaacagt
     4141 cgtcgcggac aaaaacgcgc tggctggctg gctcaagatc cgcccggcgc tccggataag
     4201 cgcggactat tcttccgaag cggaaaatat caatttcatc ccgacgaatg atccggcggc
     4261 aaaaatatat tttcgcttct tggaccttca gcgcggccgc agccacatcc gcatctcgac
     4321 gagcctcgcc ctcgacacgg accaactcaa agcttatttg gaaaaagcca aagatgcttc
     4381 acgtgtcgat ccgcaaaatg cgaaactttc ttttggaggt gggtcagtga gcgccttttc
     4441 cctttccaaa gacggatatg agatcgatac ggaaaaaagt tttcaggata ttttgaaatc
     4501 cctcacacaa aatccgctca caaaggatat cactctggaa acaaatgcgc tcaagccgga
     4561 cgtcgccagt acggacctcg acaaatatgg aatcaaagaa ttgctctccg agggcgtttc
     4621 caatttcggc aactcgccgg caaaccggat ccacaatatt caagtcggtg ccgcccgctt
     4681 caacggcgtg ctcatcaaac cacaggaaga attttccttc atcgaaactc tcggtccagt
     4741 ggacgaatcc accggctatt tgcccgagct cgtcattaaa accgacaaaa ccgttcccga
     4801 attcggaggc gggatgtgcc aggtttccac cacaatgttt cgcgccgcgc tcctcgccgg
     4861 tctcaaaata accgcccgga ccaaccatgc ctatccggtt tcgtattaca acccgcaagg
     4921 cctcgacgcg accgtctata tccccaaacc tgatctcatg ttcttgaatg acacccctaa
     4981 ttatatactt gtccaaaccc atatcacagg aacacagttg tttttcgatt tctatggaac
     5041 cagcgacgga cgtcaaacca agcttgatgg gccgactgtc ctcgaaaaaa atcccgacgg
     5101 ttcgatgaaa acggatcttt ttcaaatagt ctctgacgca agtggaaacc tcatccgaaa
     5161 atcggaattc aaaagcaatt ataagtctcc ttccctgtat ccgcacccgg gcgatgaaaa
     5221 aataaccgaa aaaccgtccg attggtcaca gaaagaatgg aaggcctaca aaaaagcgaa
     5281 tggagtgtaa ataaattgac tttttgcaaa aaatatctta tactcttcga gtgtgatttt
     5341 attttgtctc tgtagttcaa tggatagaac gagggtcttc taaaccctag accgaggttc
     5401 gactcctcgc agggacacaa agacgcgctg ttagctcagt tggtagagca ggggactctt
     5461 aatcccaagg tcgcaggttc gatccctgca cagcgcacaa agaatgatat caatttaaag
     5521 catggcggcc atcgttcaat ggtaggacta cgggttgtgg tcccgttaac ttgggttcaa
     5581 ttcccaatgg tcgcccctgt gtggagttta tgcaaaaaaa ccgcgaactt gtcgcggttt
     5641 tttgttgctt ttattatgct tacgtagcat ttgacccagt attaataaaa agatacaata
     5701 aaagcaatct tagccataaa atatcaatat ggaacaaaac caaactggag aaaaaaccat
     5761 tctcattgtt gaagacgaca ccttcatggg gagtctgctc gagcgaaaat tcaagcagaa
     5821 aaattttcag attttgcggg cgatgaatgt ggacgaagcc agaaaattca tcgagtccgg
     5881 accggtcagc ttgatactgc tcgacatcat cctccctgga acggacggga tcacttttct
     5941 caaggaactc aagctgaatc cgcaattcaa ggacattccc gtgatcatca cctcaaactt
     6001 gggaaaggag gaggaaatcg agaaaggtat cgccgaagga gcaacagact atatcgtcaa
     6061 agcccacaca actccgggag aaatcgtcga aaaggttgaa aaaattcttg gaatatgatg
     6121 gatcaagaaa aaaacaatct tccgagccaa aagcatatct ggctgattca gatcgtcttt
     6181 gcggttgccg ttgccgcaat gttgtttctg ggctatattc cgactgtttc agccgttctt
     6241 ctttttttcg tc
//
LOCUS       DATKGS010000084         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_18892, whole genome shotgun sequence.
ACCESSION   DATKGS010000084 DATKGS010000000
VERSION     DATKGS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18892"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VMQ48_02105"
     CDS             <1..297
                     /locus_tag="VMQ48_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUD08864.1"
                     /translation="GFDESDGWRYTAPVGTELGVSPYGVLNMAGNVYEWTNNDFNAYP
                     ENDKYIQEDFEKGFKTLRGGAYDDGISEQRTSTRCGFRKDYKDVDVGFRCVRSK"
     gene            479..>2279
                     /locus_tag="VMQ48_02110"
     CDS             479..>2279
                     /locus_tag="VMQ48_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HUD08865.1"
                     /translation="MPNKKISSIVAIFAATIAVFSAAKFARATDTSHVIINEIQTAGA
                     SSNDEFVELFNPTDTPVILDGFKLGKKTKSGSESTLLSSATSTKFLGIIPAHGYFLIA
                     HPDYKGSLPADLAYSSSSSISSDNTLLLYDKSGTLLDKVGFGAAIDFEGSPAANPSSN
                     QSIERNDFADTNNNAADFSINNVPLPQNSTVVETGNKSGSGNGDTSKDEPRSSDNTTD
                     GLTKCTTSSRDIKLSEVFPYPSSGDEFVEITNTGGSCVDVSGWKVMDEAGHKKTFPEN
                     SILEPGKYLYLEGNLYLNNDSDAAYLLDENGSTQNDALDKVFYEKAKENFSYALSDGI
                     FSWTSSATPGEKNIIAIPKAAEKNTTETNNSQEATENYTFDANIYLNEIMPNPKDGSD
                     GEYIEIASGGAEPVDLFGWRVKDASKSKGYQFKEHIILDPGEYLAIYRPDSKIALNNS
                     DESVYLYDPDGKITSSVSYDTSQKNASYNFDGENWKWSKYLTPGKENRFDSPPKVDIK
                     KPKHAYKDLLTEFSATAKDKETKKLKYSWNFGDGSKSTLAKTSHKYLATGRFAVTLSV
                     TDDSQTVEKSFSIEVKKYPRPNIEIVKIIPNPTG"
BASE COUNT          722 a          541 c          492 g          524 t
ORIGIN      
        1 ggttttgacg aaagcgacgg ctggaggtat acggcgcctg ttggcacaga gctgggcgtg
       61 agtccctacg gagttttgaa tatggcgggc aatgtttatg aatggacaaa caatgatttc
      121 aacgcctatc cggaaaatga caaatacatc caggaagatt tcgaaaaggg cttcaaaaca
      181 ctccggggtg gagcctacga cgacgggata agcgaacagc gcacatccac aagatgcggc
      241 tttcggaaag attacaaaga cgtggatgtc ggctttcgct gcgtcaggag caaataaaac
      301 ccatgtctcc cccttttcta aagggggact aagggggatt tctgcgatta aattcggaaa
      361 tccctctcca gctctccctt taggaaaggg agagagcact attcagaccg taatacgcat
      421 ttattacggt ctttttttgt ataatgtgcg tgaaattaat atttttaaca aaaatcccat
      481 gcccaacaaa aaaatctcct ccatcgtggc tatttttgca gccaccatcg ccgttttttc
      541 cgccgcaaaa ttcgcccgcg caacagatac atcgcatgtc attatcaacg aaattcaaac
      601 agctggcgcc agcagcaatg acgagtttgt cgaacttttc aatccaactg acacaccggt
      661 tattttggac gggttcaaat tgggcaaaaa aacaaagtcc ggctcagaaa gcacgctttt
      721 gagctcggcc acttcgacca agtttttggg catcattccg gcccatggat attttctgat
      781 cgctcatccg gactacaagg gatcgcttcc ggctgacctc gcttattcat cgtcgtcaag
      841 catttccagc gacaacactc ttctcctgta tgacaaatca gggacgcttt tggacaaagt
      901 tggatttgga gcggccattg attttgaggg atctccggca gcaaatccca gcagcaatca
      961 aagtattgag agaaacgatt ttgccgatac aaacaacaac gctgctgatt tttccataaa
     1021 taacgttcct ttgccgcaaa atagtaccgt tgttgaaact ggcaataaaa gcggatcagg
     1081 caacggcgat acaagcaaag atgaaccgag aagtagtgat aataccacgg atggcttgac
     1141 gaaatgcacc acctcctcaa gagatataaa attaagtgaa gtattcccct atccctcaag
     1201 cggcgatgag tttgttgaaa tcacaaatac cggcggaagc tgcgtcgatg tctccggctg
     1261 gaaagtgatg gacgaggccg gccacaaaaa aacatttccc gaaaattcaa ttctggagcc
     1321 cggcaaatat ctctatctgg aagggaatct ctatctcaac aatgattccg acgcggccta
     1381 tcttctggat gaaaacggca gcacacaaaa tgatgcactc gacaaagttt tttatgaaaa
     1441 ggcgaaagaa aacttttcct atgccttgtc ggacggaata ttttcctgga catcatctgc
     1501 cactccagga gaaaaaaaca taattgcaat tcctaaagcc gctgaaaaaa atacaaccga
     1561 aacaaacaat tctcaagaag caaccgaaaa ttacaccttt gacgcaaata tttatcttaa
     1621 cgaaatcatg cccaatccca aagacggatc tgatggcgaa tatattgaga ttgcaagcgg
     1681 cggcgccgaa ccagtcgatc ttttcggctg gcgcgtcaaa gacgcctcca aaagcaaagg
     1741 atatcaattc aaagaacata tcatcttgga tccgggcgaa tatttggcaa tatacagacc
     1801 cgattcgaaa atagcgctca ataactcgga tgaatcagta tatctgtatg atcctgatgg
     1861 gaaaatcacc tcaagtgttt cgtatgacac atcccagaaa aatgcttcgt ataatttcga
     1921 cggagaaaat tggaaatgga gcaaatatct cacccccggg aaagaaaaca ggttcgattc
     1981 cccgccaaaa gtggatatca aaaaaccaaa gcacgcttac aaagacctcc tcacggaatt
     2041 ttcagcgact gccaaggaca aagaaacgaa aaagttgaaa tactcttgga atttcgggga
     2101 cggaagcaaa agcaccctcg cgaaaacctc gcacaagtat ctcgccaccg gaaggttcgc
     2161 ggtgacgctc tccgtgacgg acgactcgca aacagttgaa aaaagtttct cgattgaagt
     2221 gaaaaaatat ccccggccaa acattgaaat tgttaaaatc attcccaacc ccactggcc
//
LOCUS       DATKGS010000085         2843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_18930, whole genome shotgun sequence.
ACCESSION   DATKGS010000085 DATKGS010000000
VERSION     DATKGS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18930"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VMQ48_02115"
     CDS             complement(<1..218)
                     /locus_tag="VMQ48_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08866.1"
                     /translation="MQGYVANIEKETEENDNFRKVLYTAKFSQLVVMALKPMEEIGEE
                     THTLDQFIRIEKGTGKAVLDGIEHEIADG"
     gene            complement(301..1038)
                     /locus_tag="VMQ48_02120"
     CDS             complement(301..1038)
                     /locus_tag="VMQ48_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD08867.1"
                     /translation="MKLSFVIPAHNEENYIAKCLESVIREVEKTRRDVEIIVVNNAST
                     DKTREIVLSFPEVKLVDEPRKGLPQARQAGFLASSGDLIANVDADSVIPSFWIEKVFD
                     HFSRNEKLVALSGPYIYYDYSKTKNRLVKAFYYPGYLLNRTNHIFFGKAAMLQGGNFI
                     LKRSALENIGGFNTDIKFYGEDTDIARRIKNQGQVIFTYDLPMHTSARRLKKEGLVKM
                     GAKYGANFIWTIVFKKPLTKKYIDVKD"
     gene            complement(1035..2231)
                     /locus_tag="VMQ48_02125"
     CDS             complement(1035..2231)
                     /locus_tag="VMQ48_02125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08868.1"
                     /translation="MRIAIYSDNFFPEMSGISDSIIALAKELSRRGHQANFYVPRYSK
                     KNFLISKLKPEELDLGKNIEIFRLPSISYPSTPTKQGRIVLPVLSSYRHLKKFNPDVI
                     YTQDFFFAGLEALAVARILKKPFIGTNHTPISEFLKYSPIHWKWIEKLILKYVSWYYN
                     KCLWISAPSQPILEEMKKFGLKRKSRAVSNPIYISEYKPVSDQVREELKKKFVFSERA
                     ILYTGRLADEKNIDVIIRAISLVKKKFPSITFAVTGHGNAETKLKKLAKELGLEKDVL
                     FLGYVETSEFVKIYQASDIFAVMSTAETQCISMMQAMATGIPVIGANSWGLPDYINSE
                     NGYLIEPGDHKTLAEKIIYLLENPTERKRLGEGGLKFVQKFGIKNIADEWEKIFNQFA
                     NNHRPE"
     gene            complement(2243..>2843)
                     /locus_tag="VMQ48_02130"
     CDS             complement(2243..>2843)
                     /locus_tag="VMQ48_02130"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUD08869.1"
                     /translation="FTTFAGAFQWVIAHGYVLIFIAMLIEGPIVTVAAAFAAALGYFD
                     VFIVFILSFLGDIVADVIYYFIGYFSRLAVIEKFGRFFGLTEKRMHRIEELLNGHSVK
                     TLIALKLTPVLPTPGLMLVGATKMPIKRFVKICSIIIFPRTIFLVIMGYYFGAAYEVM
                     AKQFEKNYFILIAIVAIVAIVYYLFSQLLAYLGRRVEKI"
BASE COUNT          761 a          676 c          597 g          809 t
ORIGIN      
        1 ccgtctgcga tttcgtgctc aatgccgtca agaacggcct tgcccgtccc cttctcaatg
       61 cggatgaatt ggtcaagcgt atgcgtttct tcgccgatct cttccatcgg cttgagagcc
      121 atcaccacca gctggctgaa cttggccgta tagagaacct tgcgaaaatt atcgttttct
      181 tcggtttctt tttcgatgtt cgcgacatag ccttgcataa ttgtattctt aaaatttaat
      241 tagcctactg caacataaac tattacaccc gcaatctttt tttctttcta aagaattttt
      301 tcagtcttta acgtctatat atttctttgt aagcggtttt ttgaaaacga tggtccagat
      361 aaaattggcg ccgtatttcg ctcccatttt cacaagaccc tcctttttca gtcttcgggc
      421 ggaagtatgc atgggaagat cataagtaaa aatcacctgg ccctggttct tgatgcggcg
      481 cgcgatatcc gtgtcttcgc cataaaattt tatgtccgtg ttgaacccgc cgatattttc
      541 aagcgccgaa cgtttcagaa tgaaatttcc cccttggagc atcgccgcct ttccaaaaaa
      601 aatatgattt gtccgattca aaagataccc gggataatag aaagccttga ccaaacgatt
      661 ctttgttttc gaataatcat aatagatata cggcccgcta agcgccacca atttttcatt
      721 gcgggaaaaa tggtcaaaaa ctttttcgat ccaaaaggaa ggaatgaccg aatccgcgtc
      781 aacattcgcg atcaaatccc cactcgaagc gagaaaaccg gcttgcctgg cttggggcaa
      841 gcccttcctg ggctcgtcga ccaattttac ttcgggaaac gaaagaacaa tttcacgggt
      901 tttgtccgtg ctggcgttgt tgacgacaat tatctcgaca tcccggcgcg tcttttctac
      961 ctctcgaatg acggattcaa ggcacttggc tatgtagttt tcctcattgt gcgccggaat
     1021 gacaaaactc agtttcattc tggtcgatgg ttatttgcaa attggttgaa tattttttcc
     1081 cactcgtcgg ctatgttctt tataccgaat ttttgcacaa atttcaaccc gccctcgccc
     1141 agcctctttc tttccgtcgg attttccaga agatagatta ttttttccgc caatgttttg
     1201 tgatctcccg gttcgatcag atacccgttt tcgctgttga tataatccgg caatccccaa
     1261 ctattggctc cgatcacggg aattccggtc gccatagcct gcatcatact gatgcactgg
     1321 gtttcggccg tactcatcac ggcaaaaata tcagaagctt ggtatatttt cacaaactcg
     1381 ctcgtctcca cataacccaa aaacagaacg tctttttcga gacccagttc cttggctaat
     1441 tttttgagct tagtctccgc gtttccatgc ccggtcacag caaaagtgat gctgggaaat
     1501 tttttcttga ccaaggatat ggcccggata atcacgtcaa tatttttttc atccgccaat
     1561 cgccccgtat agagaatcgc gcgctccgaa aaaacaaact tttttttcag ttcctcccgc
     1621 acttggtcac ttaccggttt gtattccgaa atatatatgg gatttgagac ggcacgactt
     1681 tttctcttga gtccgaattt tttcatctct tcgagaatcg gctgactggg cgcagaaatc
     1741 caaaggcatt tattgtaata ccaactcaca tatttcagga tcagcttttc aatccacttc
     1801 caatggatcg gagagtactt caaaaattcg ctgataggcg tgtgattggt gccaataaac
     1861 ggctttttga gaatccgcgc aaccgcgagc gcttcaagtc cggcgaaaaa gaaatcttgc
     1921 gtatagatca catccggatt gaattttttg aggtgtcggt agctcgaaag aacgggcagt
     1981 acgatccgtc cttgtttagt cggcgtggag ggataggaaa tagagggtag ccggaaaatc
     2041 tcgatgtttt ttccaagatc cagttcttct ggcttcaatt tggaaatgag gaaatttttt
     2101 ttcgaataac ggggaacata gaaattagct tggtgccccc ttcgggaaag ctctttcgca
     2161 agcgcgataa tcgagtcgga aatgccgctc atctcgggaa agaaattgtc agaatatatg
     2221 gctattctca tcaaatttga tcttaaattt tttcaaccct tctgccgaga taggccagga
     2281 gctggctgaa aagataataa acgatagcga cgattgcgac aatggcaatg agaataaaat
     2341 agtttttttc aaactgcttg gccatcactt cataggccgc cccgaaatag tatcccatga
     2401 tcaccaaaaa aatcgtccgg ggaaaaatga tgatcgaaca tattttcacg aatcttttga
     2461 tcggcatttt tgtcgcgccg acaagcataa ggccgggcgt gggaagaaca ggtgtgagct
     2521 tcagcgcaat gagagttttc acggaatggc cattgagcag ctcctcgatg cgatgcatcc
     2581 gtttttccgt gagtccgaaa aatctcccga atttttcaat cacagccaaa cggctgaaat
     2641 agccgatgaa ataatatatc acatcagcga ctatatctcc cagaaaagaa aggataaaaa
     2701 caatgaaaac gtcgaaatat cccagggccg cagcaaaggc cgccgcgacc gtcacaatcg
     2761 gaccttcgat gagcatcgca atgaaaatca aaacataccc gtgtgcgatg acccactgaa
     2821 aggcgccggc aaaagtagtg aaa
//
LOCUS       DATKGS010000086         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_9615, whole genome shotgun sequence.
ACCESSION   DATKGS010000086 DATKGS010000000
VERSION     DATKGS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9615"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VMQ48_02135"
     CDS             complement(<1..741)
                     /locus_tag="VMQ48_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HUD08870.1"
                     /translation="MKQSQLFTKTQKNAPKDEVSTNAQLLIRGGFVDKLMAGAYTILP
                     LGWRVLKKIEQVIREEMNAIGGQEIFMPSLQPKENWETTGRWSTMDDLYKVKDASDRE
                     YALGPTHEEIISPLMKKFVNSYKDLPFAAYQFQNKFRMELRAKSGILRGKEFLMKDLY
                     SFHISQEDLDKYYEKAKQAYKNIFDRTGIGEKTFLTFASGGSFSKYSHEFQTETDAGE
                     DIIFVCSECSTAYNKEISDEISHCPECKN"
     gene            complement(738..1577)
                     /locus_tag="VMQ48_02140"
     CDS             complement(738..1577)
                     /locus_tag="VMQ48_02140"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HUD08871.1"
                     /translation="MKITLGKHSGFCFGVKRAYDLTCVNSKDCDDVYILGKLVHNNNV
                     CQDLYKRGIKEIKLLSDIKEGTVIFTAHGVGPGFYEKASARGLKIIDTTCPKVMKAQR
                     LAKSFAEKKYQVLIFGDKNHKEVKSIFEWSGRHAKIVGSLAEAEKLKLDKAKKYCLVA
                     QTTQNVEEFKKIIAYFQKNLKNFVYFNTICPSTDDRQKEVRELAKSNEAVIVIGGKDS
                     ANSRMLYEIAKSINPKSYFVENVGQLKKSWFSDIKKIAVSAGASTPEWVIEEVIGKIN
                     SYT"
     gene            complement(1623..>2233)
                     /locus_tag="VMQ48_02145"
     CDS             complement(1623..>2233)
                     /locus_tag="VMQ48_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUD08872.1"
                     /translation="LWNAARFMAMNVKKAELLKATDAVEPADAWILDELNKTIAKTTK
                     AFENYAYAKAKEEIDSFFWSKFADYYVEFVKYRFFGEDEKSKKIGEKTIATVFFNVVK
                     LYAPIMPFVTEEIFQTWFKHLEKVKSLHISQWPEEIKLKKSPDISDFGSAIKAVDEIR
                     KHKTKNGLSLGAEIDSIKLKTKTNLKKYGEFLRKVGRVGKII"
BASE COUNT          514 a          516 c          455 g          748 t
ORIGIN      
        1 atttttgcat tccgggcaat ggcttatttc atcgctgatt tccttgttat aagccgtgct
       61 gcattcggaa caaacgaaaa ttatgtcttc tcccgcatca gtttcagtct ggaactcgtg
      121 cgaatatttt gagaatgatc cgcccgaagc aaaagtgaga aatgtttttt cgccgattcc
      181 ggtgcggtca aaaatatttt tgtaggcctg tttggccttt tcataatatt tatcgagatc
      241 ttcttggctg atatgaaagg agtaaagatc tttcatcaga aattccttcc cgcgcaaaat
      301 gccggatttg gctcgcagct ccatccggaa tttgttctgg aattgatagg cggcgaaagg
      361 cagatctttg tatgaattta cgaatttttt catgagcggc gagattattt cttcgtgagt
      421 cggcccaagg gcgtactcgc ggtcactcgc gtctttcact ttatagaggt cgtccatcgt
      481 actccagcgc cctgtcgttt cccaattttc tttgggctgg agagacggca tgaaaatttc
      541 ctgtccgccg atggcgttca tttcctcacg aatgacctgt tcaatctttt tcagtacgcg
      601 ccagccgagc ggcaggatcg tataggcgcc cgccatcaat ttgtcgacaa aaccgccccg
      661 gatcaaaagc tgagcgtttg tgctcacctc atccttgggc gcgttttttt gagttttagt
      721 gaaaagttgg gattgcttca tgtatatgaa tttattttgc cgataacctc ttcaatcacc
      781 cactcgggag tcgaagcccc agcggaaaca gctatctttt ttatatcact aaaccagctt
      841 ttcttcaatt gcccgacatt ttccacaaaa tatgattttg ggttgattga ttttgcgatt
      901 tcatagagca tccttgaatt ggcgctgtct tttccaccga tgacaatcac cgcctcgttg
      961 ctttttgcaa gttcgcgcac ttctttttgt cggtcatccg tcgatgggca gatcgtattg
     1021 aaataaacaa agtttttcag atttttttgg aaataagcga ttattttttt gaattcttcg
     1081 acattttggg tggtttgggc gaccaaacaa tattttttgg ccttgtcgag tttcagcttt
     1141 tccgcttctg ctaggctccc gactatcttg gcatgtctcc ctgaccattc aaaaatactc
     1201 ttcacttcct tgtggttttt gtcgccaaaa ataagtactt gatatttttt ctcggcgaaa
     1261 cttttggcca gccgttgcgc tttcatcacc ttgggacagg tagtgtcgat gatcttgagc
     1321 cctctggcgc tcgctttttc atagaagcct ggcccgacgc catgggcggt aaagatcact
     1381 gttccttctt tgatatcaga taacaatttt atttctttta tcccccgttt gtataaatct
     1441 tggcagacat tattgttgtg gacaagtttg cctaggatat acacgtcatc gcaatctttt
     1501 gaattcacac acgtcaaatc ataggcgcgc ttcacgccga aacaaaaacc ggaatgcttg
     1561 ccaagggtga ttttcataat tttaaggcta aaccttatct tttttaaggt ttagccttaa
     1621 aattatatta ttttccccac tcttcctact ttcctcaaaa actccccgta ttttttcaag
     1681 tttgttttgg ttttgagttt aatggaatca atctctgcgc cgagggaaag gccgtttttt
     1741 gttttgtgct tcctgatctc atcgaccgcc ttgatcgcgc tcccgaaatc ggaaatatcc
     1801 ggtgattttt tgagcttgat ttcttccggc cattgggaga tgtgaagact tttcactttt
     1861 tccaaatgct tgaaccaggt ctgaaaaatt tcttccgtca caaacggcat gatcggagca
     1921 tagagtttca caacattgaa aaataccgtc gcgattgtct tctcgccaat tttttttgac
     1981 ttttcatctt cgccaaaaaa acgatatttc acgaactcga cataatagtc ggcaaacttc
     2041 gaccagaaaa agctgtcgat ctcttctttg gctttggcat aggcataatt ttcaaaagct
     2101 ttggtcgttt tcgcgatcgt tttattgagc tcgtcgagaa tccaagcatc cgccggttca
     2161 accgcatctg tcgctttgag caactccgct tttttcacgt tcatcgccat gaagcgggcg
     2221 gcattccaaa gct
//
LOCUS       DATKGS010000087         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_120151, whole genome shotgun sequence.
ACCESSION   DATKGS010000087 DATKGS010000000
VERSION     DATKGS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120151"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VMQ48_02150"
     CDS             <1..442
                     /locus_tag="VMQ48_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08873.1"
                     /translation="YENTNANPYLKATLGKNIPMLILNISPKEWTGMKNEDKEKKQQE
                     AFQKLEQGADVDFEAEIDGKKIKIQAEGLSLEEYEIFQRIYFEKNPGKHLDEKGWTWL
                     PKSRSGSRVVRSSWSPDDRRLDVDADDPGSALDALGLRLSRSFS"
     gene            complement(828..1247)
                     /locus_tag="VMQ48_02155"
     CDS             complement(828..1247)
                     /locus_tag="VMQ48_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUD08874.1"
                     /translation="MNNHKNFESIEIPLVKKLYELYKYAHKSVSVFPKHERYSLGEKI
                     ETNILEAIEAVIFGNAQPKNFKDAYLLRANAKVEVLKILFRLAMDNEFINSTQYLKTE
                     EYLCESGKMLGGWIKYLHSGAAGYAQNCNVDRKSMRK"
     gene            1236..2270
                     /locus_tag="VMQ48_02160"
     CDS             1236..2270
                     /locus_tag="VMQ48_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HUD08875.1"
                     /translation="MIVHMNVDYDELTSVENLFYSWRAYKKGKSRKADVMKFERHLED
                     NLFSLHEKLKTGSYSHAGYSYFRISDPKKRDIWKAAVGDRIVHQALFEYLCKIYEPLF
                     IDLSFSSRKNKGTHKAVLALAESAKNFRKNREKCLAMKCDVRKYFENIDHGILLEILR
                     KNIGDDKIFNLLKTVVKSFNNNVSRGIPLGNITSQIFANIYLNELDKFATGGLELEHY
                     VRYNDDFIVLENNEEKLFADIEKIRIFLKEKMSLKLPKEKIIFRKLNWGIDFCGCVVL
                     PNAILIRNKTKGRMFEKIKAISRKLRAEKISLNDARKTVNSYFGLLSHCKAHNLREKI
                     RNRYLYEHIF"
BASE COUNT          768 a          481 c          597 g          686 t
ORIGIN      
        1 ctatgaaaat acaaatgcta atccgtatct caaggcgacg ctgggaaaga atattcctat
       61 gttgattttg aatatttccc cgaaagaatg gacgggtatg aaaaatgaag ataaagaaaa
      121 aaaacaacaa gaggcttttc aaaaactcga gcaaggagct gatgttgatt tcgaagcgga
      181 gattgacgga aagaaaataa aaattcaggc cgaagggctt tctctggaag aatacgaaat
      241 cttccaaaga atatatttcg agaaaaatcc gggaaaacac cttgatgaaa aaggctggac
      301 ttggcttccc aaatctcgct ccggttcccg ggtcgtgcgc tcgagctgga gtccggacga
      361 tcggcggctg gatgtcgatg ccgatgatcc cggcagtgcg cttgacgctc ttggcctccg
      421 cctctcccgt agtttctcat gatttaagtt ttggatattt ttgattttct tttttatttt
      481 tactttttat tcaatcaggc aagttcgggt cagtacgacc gatttcgtcg gtttgcccga
      541 aacgatttcc aatcaaaaag acggccgatc agcaaagtcg gacggtacag attggattcg
      601 cgccaggaga aactacagcc tttctttcgg gcgatcgggt cgccctgata aatcgcgatt
      661 agcgaaggcg caacggacaa ggaagggcta aaagaggcgg ggctccggtt cccgggtcgt
      721 gaactcgaac tggaatccgg acgatcggca gctgaatgtc aatgccaatg atcccggcaa
      781 tgcgaatgac aatcttggcc tccgcctctc ccgtagtttt tgctctgcta ttttctcata
      841 ctcttgcggt caacattgca attttgcgcg taccccgcgg cgccggagtg caaatatttt
      901 atccagccgc ccagcatttt tccggattcg cagagatatt cttcggtctt gagatattgc
      961 gtcgagttga taaattcgtt gtccatagcc aggcgaaaaa gtattttcaa gacttcgact
     1021 ttggcgttgg cccgcaaaag gtaagcatct ttgaagtttt tcggctgggc gtttccaaaa
     1081 atgaccgctt cgattgcctc caaaatattc gtttcgattt tttctcccag gctgtatctc
     1141 tcatgcttcg ggaaaacaga aacggatttg tgggcatatt tgtaaagttc atacagcttc
     1201 ttgaccagcg gaatttcgat tgactcaaaa tttttgtgat tgttcatatg aatgttgatt
     1261 atgatgaatt aacaagcgtg gaaaatcttt tttattcatg gcgcgcgtat aaaaagggaa
     1321 aaagccggaa agcggatgta atgaaattcg aaaggcattt ggaagataat ttgttttcgc
     1381 tgcatgaaaa attaaaaacc ggaagttaca gccatgccgg ctattcctat ttcagaatat
     1441 cagatcccaa gaaaagggat atctggaaag cggcggtcgg cgacagaatc gtgcatcagg
     1501 cgctttttga gtatctttgc aaaatatacg agcctctttt catcgatctt tctttttctt
     1561 ccagaaagaa caaggggacg cacaaggcgg tgttggcgct ggcggaatcc gcaaagaatt
     1621 tccggaagaa cagggaaaaa tgcctggcta tgaaatgcga tgtcaggaaa tattttgaaa
     1681 atatagatca cggaattttg ctggaaattt tgcgaaaaaa tatcggagat gacaaaattt
     1741 tcaatttgct gaaaactgtt gtgaaaagtt tcaacaacaa tgtgagcagg ggcattcctt
     1801 tgggcaatat aaccagtcaa atctttgcca atatatatct gaatgaactg gacaaattcg
     1861 ccacaggcgg cttggaattg gagcattatg tcagatataa cgatgatttc attgtcttgg
     1921 aaaacaatga ggaaaaactt tttgcggata tcgaaaagat cagaatattt ttgaaagaaa
     1981 agatgtcgct caaattgcca aaagaaaaaa taatttttcg gaaattgaat tgggggatag
     2041 atttttgcgg ttgcgttgtt ttgccaaacg ccatattgat cagaaacaaa accaaaggca
     2101 ggatgtttga aaaaataaaa gcaatttccc ggaaactgcg cgccgaaaaa atttcattga
     2161 acgatgcgcg aaaaaccgtc aattcatact tcggattgct ttcccattgc aaagcgcata
     2221 atttgaggga aaagataaga aacagatatt tgtatgagca tattttttag ccggatttcg
     2281 ggtagcggga ttgtgaggcg gacgtgaagt gaaaaaaata tcagggaaag gagtagtaat
     2341 agtcaacgcg ggggaaattg tgcgaaaaag caaaaacatc ccacaccaca gagataaaaa
     2401 tacatctggg ggattaaaat ctattgagca ttaaaacccg gactgtggta cgggataagt
     2461 ttgcctattt tgggtatttt gctaatataa cctcacctct tatcctctcc ttgaaaagga
     2521 gaggaggcta tt
//
LOCUS       DATKGS010000088         4419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_109601, whole genome shotgun sequence.
ACCESSION   DATKGS010000088 DATKGS010000000
VERSION     DATKGS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109601"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..652)
                     /gene="frr"
                     /locus_tag="VMQ48_02165"
     CDS             complement(95..652)
                     /gene="frr"
                     /locus_tag="VMQ48_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HUD08876.1"
                     /translation="MLEQTVSNAKPKMEKGVERFKEEINKLRTGRANPAMVESLSVEY
                     YGAKTPLKQIASINVPEPRLIVVQPWDKDSLVNIEKAINEAQLGFNPVNDGQVIRLAI
                     PPLNEERRTELVKTLGKYAEEAKVEVRKAREVAWDEIQEMVKDGKLAEDDKFRGKDML
                     QKGVDEYNGKIEEIREKKEKEMMEI"
     gene            complement(675..1724)
                     /locus_tag="VMQ48_02170"
     CDS             complement(675..1724)
                     /locus_tag="VMQ48_02170"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HUD08877.1"
                     /translation="MNKTKILGLFASVFLLTGCSLVSSSSDGGVFRSDDGGKSFSQKV
                     TIDDKTKISSVDVLSLAVNPQNGNEVYIGTVSSGIFKTTDAGEKWQVLKVSTSMVTKV
                     YAMAVDPTNANTVYAAAIVDGRGKILKSTDAGANWKEIYTEPSSGSFVLALAIDPAGS
                     GKIFGGTTEGQIIFSDNGGDTWKNLYKTQGGVFKIAIDSTNSNLEYFAVSQGGLIRTR
                     DGGNNFEDLGEKSNLNGQQQLGSPTAITVNPNRTNWVYVGTTAGLFRSKNGGDDWEKV
                     KVLNSPQSNVVRGIAINPQNSDEIAYGAAQAFYKSVDGGQSWATTQLGGSRTAEAVAY
                     NRQNPGVIYVGMNKR"
     gene            complement(1739..2539)
                     /locus_tag="VMQ48_02175"
     CDS             complement(1739..2539)
                     /locus_tag="VMQ48_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08878.1"
                     /translation="MKINPKIKSYLDRLVDSASRPSSYIFAGAGESEKEEAAYYFISK
                     LAGKFGDEEFLARIKAKNHPDVVIIEPEIEEKKGKTREKEISISQVRGSLERLKFFPY
                     ELKTKFCIIKRSQKMNAEAANALLKSLEEPSEKAIFLLLVDNIDSLLPTIASRCAVLR
                     FPQTNLPEWSEENREHLKNIFRQEIFERFDYIEKISKNKNEMIAALKDWELVMAESLR
                     KLVGGDEDRRKIGKVIELVEDTREAINRLEHTNANARGVGEELVLRMS"
     gene            complement(2547..3707)
                     /locus_tag="VMQ48_02180"
     CDS             complement(2547..3707)
                     /locus_tag="VMQ48_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD08879.1"
                     /translation="MNADKFKVALVHDFLNQYGGAERVLLALAKIFPEAPIYTLLYDP
                     KKMRGKFKNADVRPSFLQKFPKILKKRPKWLLPFLPTAPETFNLRDYDLVISSSGAWS
                     KGIIVKPKTIHICYCHSPMRFAWDWNEKYLGEQNLGKGRRIFARLLLNYVRMWDKAAA
                     DRPDFFIANSRATETRIKKYYGRESEVVFPPVEIGSVEVGPQTHEGSGPTSTRDYFLI
                     VSRLSPYKKIGVAVEAMNKLNLPLVVIGEGAPKYVKYLKSIAGPKTKFLGWKSDEETK
                     KYFAACRAFIFPGEDDFGIAPVEAMGFGKPVIALRKGGATETVIEGETGEFFDEPAIE
                     VLADAVRRFQENEKNYDSAKIRAQAEKFSKDKFVENIERVIGEMVNRYNLEN"
     gene            complement(3704..>4419)
                     /locus_tag="VMQ48_02185"
     CDS             complement(3704..>4419)
                     /locus_tag="VMQ48_02185"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUD08880.1"
                     /translation="GRWSIRNSCRCARRIITVSENTKNDLIKLYRISGDKIDVIYEGC
                     SNDNFKSNLNDKILNYKNYFLFIGRLEDRKNISGIVEAFEILKEKHKIPHKLILVGKF
                     GFGGEKIKGKIDSSEYKSDMILTGYVSDEDKFYLLANADVFLFPTFYEGFGLPILEAQ
                     SVGTPVITSDVSSMPEVADHSAVLVDPKDVNAIAEAAHKLISDESYKNDIIQKGLENV
                     KRFSWEKCAREVADIIKNK"
BASE COUNT         1052 a         1198 c          813 g         1356 t
ORIGIN      
        1 ttctaaatga ctaaatgatt ttgtttttga aattgaatca ttgggtcatt gaaaattgtt
       61 tgaaaatatg taaattgaaa attgaaaatt tccgtcagat ttccatcatc tctttttctt
      121 ttttttcccg gatttcttcg atctttccat tgtattcatc tacgcccttc tgaagcatat
      181 cctttccgcg aaatttgtca tcctcggcca gttttccatc cttgaccatc tcctggatct
      241 catcccaggc aacttcgcgc gcctttcgca cctctacttt ggcttcttct gcatattttc
      301 cgagagtttt cacgagctcc gtgcgacgct cttcattgag aggcggtatg gccagacgga
      361 tcacttggcc gtcgttcact ggattgaatc ccagctgagc ttcattgatc gctttttcaa
      421 tgttcaccag actgtctttg tcccatggct ggacaacgat gagccgcggt tcgggaacat
      481 tgatgcttgc tatctgcttc aaaggagttt ttgcgccata gtattcaacc gacaggcttt
      541 ccaccatcgc cggattggcc cgtcctgtcc tcaatttgtt tatttcttcc ttgaaacgtt
      601 cgactccttt ttccattttt ggttttgcat tggaaacagt ttgttcgagc atatttttat
      661 tataattatt agcactaccg cttgttcatt cctacgtata tcacgcccgg gttctgtcgg
      721 ttgtacgcaa ccgcctcggc tgtccggctt ccgccaagtt gcgtcgtcgc ccagctttgc
      781 ccgccatcca cggacttata aaaagcctgc gccgctccat aggctatctc atccgaattc
      841 tgggggttga tcgcaatccc tctgacgaca ttgctttgcg ggctgttcaa caccttcact
      901 ttttcccagt catccccgcc gtttttgctt cgaaacaaac cggcggtcgt tccaacataa
      961 acccaattcg tcctgttggg attaactgta attgccgtcg ggcttcccag ctgctgctgg
     1021 ccgttcaaat tgctcttttc tccaagatct tcaaaattat tccctccatc ccgagtcctg
     1081 atgagcccgc cctggctcac cgcaaaatat tccagattag aattcgtcga atcaatcgct
     1141 atcttgaaaa cgcctccctg ggtcttatac agatttttcc aagtatctcc gccattgtcc
     1201 gaaaaaatga tctggccttc cgtcgttccg ccaaaaatct tccctgaccc cgccggatca
     1261 attgccagag ctaaaacaaa cgacccgctc gacggctcgg tatagatctc tttccaattc
     1321 gcacccgcgt ccgttgattt caaaattttc ccccggccat ccacgatcgc cgccgcatag
     1381 actgtattcg cgttggtcgg atcaaccgcc atcgcataga cttttgtcac catgctcgtc
     1441 gaaactttga gcacttgcca tttttcaccg gcatcggtcg ttttgaatat gccgcttgaa
     1501 actgtgccga tataaacttc gttcccgttt tggggattca ctgcgagact cagcacatca
     1561 acagagctga ttttcgtttt atcatcgata gtcacttttt gagaaaagct cttcccgcca
     1621 tcatccgagc gaaaaacccc tccatcggag gaactggaaa cgagagaaca gccggtgaga
     1681 agaaacactg aagcaaataa gcctagtatt tttgttttgt tcatatgatt attatagact
     1741 aactcatcct taaaaccaac tcctccccca ctccccgcgc atttgcgttt gtgtgctcga
     1801 gccggttgat cgcttccctt gtatcctcga caagttcgat gacttttcca atttttctcc
     1861 gatcttcatc gcctccgacc aatttccgca agctttctgc catcaccagc tcccagtctt
     1921 tcagtgctgc aatcatttca tttttgtttt tcgaaatctt ttcaatataa tcaaaccttt
     1981 caaaaatttc ctgtcgaaaa atatttttca gatgctctct attttcttcg ctccattccg
     2041 gcaaatttgt ttgcggaaat cgaagaacag cgcagcggga agcaatcgtg ggcaaaagcg
     2101 aatcgatatt atcaacaaga agcagaaata tcgctttttc cgacggctct tcaagagatt
     2161 tgagcaatgc gttcgccgct tcggcgttca ttttttggga tcttttgata atacaaaact
     2221 ttgtcttgag ctcgtatgga aaaaatttca gcctttcgag cgaaccccgg acttgagaaa
     2281 tactgatttc tttttccctt gtttttcctt ttttttcctc aatttccggc tcgatgatca
     2341 ccacgtcggg atggtttttc gcctttattc tggccaaaaa ttcctcgtct ccaaatttcc
     2401 ccgcgagttt tgaaatgaag taatatgccg cctcctcttt ttcactttcc cccgctcccg
     2461 caaaaatata cgaactcggc cggctggcgg aatccacgag acggtcaaga taggatttta
     2521 ttttgggatt aattttcata atatccctaa ttttccaaat tataccgatt taccatctct
     2581 ccaatcaccc tttcaatatt ctccacaaac ttatctttcg aaaatttttc ggcttgggcg
     2641 cggattttgg cggagtcata gtttttttcg ttttcttgga accggcgcac ggcgtcagcc
     2701 agcacttcga tcgccggctc gtcaaaaaat tcgcctgtct cgccctcaat gaccgtttcc
     2761 gtcgctccac ctttgcgaag cgcaatcacc ggttttccaa aacccatcgc ttcaaccggc
     2821 gcaatgccaa aatcatcctc gcccggaaaa atgaacgccc ggcaagcagc aaaatatttt
     2881 tttgtctcct cgtctgattt ccagcccaaa aacttcgtct tgggtccggc aatcgatttc
     2941 agatatttca catatttcgg cgcaccttcc ccgatcacca ccaaaggtaa attcaacttg
     3001 ttcatcgcct caaccgccac tccaatcttt ttgtaaggtg aaagccggga aacaatgaga
     3061 aaataatcgc gagtagaggt cggtccgctt ccctcgtgag tctgcggtcc gacctctacg
     3121 gatccgattt ctaccggcgg aaaaacaacc tcgctctccc ggccgtaata ttttttgatc
     3181 cgcgtctccg tcgctcttga attcgcaatg aaaaagtccg gccggtccgc cgccgctttg
     3241 tcccacatgc ggacataatt gagaagcagc cgcgcgaaaa ttctccgtcc ctttccaaga
     3301 ttttgctcgc ctagatattt ctcgttccaa tcccaggcaa agcgcatcgg gctgtggcaa
     3361 tagcaaatat gaattgtttt cggtttcacg atgatgcctt ttgaccaagc gcctgacgaa
     3421 gaaatcacca gatcatagtc tcgcaaattg aacgtttccg gcgcggtcgg caaaaaaggc
     3481 aaaagccatt ttggccgctt tttcaaaatc ttgggaaatt tttgcaaaaa agacgggcga
     3541 acatcggcat ttttgaactt tccccgcatt tttttcgggt catacagcaa agtatagatc
     3601 ggcgcctcgg gaaaaatttt cgcgagcgcc aaaagcaccc tctccgcccc gccatattgg
     3661 ttgagaaaat catgaacaag cgcgacctta aatttatctg cattcattta ttttttatta
     3721 tatccgcgac ctctctcgcg catttctccc agctgaatct tttcacgttc tccagtcctt
     3781 tttgtattat atcattttta tagctttcgt ccgaaatcag tttatgcgcc gcttcggcga
     3841 tggcgttgac gtcttttgga tcgaccaaaa ccgcactgtg atccgccact tcgggcatcg
     3901 aagaaacatc agaagtaatc accggcgtac cgacgctttg cgcctcaaga attggaagac
     3961 cgaaaccttc atagaaggtc gggaataaaa acacatctgc gtttgccagc aaataaaact
     4021 tatcctcatc gctcacataa ccggttagga tcatgtcgct tttgtattca ctgctatcaa
     4081 ttttgccctt tattttttct cctccgaaac caaacttgcc gaccaaaatc aatttgtgag
     4141 gaattttatg cttttctttc aggatttcaa aagcctcaac tattcccgaa atatttttcc
     4201 tatcttccag ccgaccaatg aacaaaaaat aattcttata atttaaaatt ttgtcattta
     4261 gatttgattt gaaattatca ttactacacc cttcataaat aacgtcaatc ttatctccag
     4321 atatccgata taattttatc aaatcattct ttgtattctc gctcaccgta ataatccgtc
     4381 tggcacaccg gcatgaattc cgaatcgacc agcgccccc
//
LOCUS       DATKGS010000089         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_96170, whole genome shotgun sequence.
ACCESSION   DATKGS010000089 DATKGS010000000
VERSION     DATKGS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96170"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VMQ48_02190"
     CDS             complement(<1..279)
                     /locus_tag="VMQ48_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08881.1"
                     /translation="MQKTKKFFKKLGDFIKHPFNRKNEPLLHTIEWVFILLAFFAVGL
                     PIINYQTRENNLGGGKLTSLGNQEATAPPSEAEKKFIGETVTEPIVDVT"
     gene            complement(397..>2198)
                     /locus_tag="VMQ48_02195"
     CDS             complement(397..>2198)
                     /locus_tag="VMQ48_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HUD08882.1"
                     /translation="GRGHYSNISPKKFDDFFLTFFSCIATLPVLVSTYKSVKNKKISV
                     DLLASIALVVSLLNKEWASAVFINLMLTSARIFGDYTERRSQNAIKSLLKLRPERVKI
                     KNGNRVVEKSISKIKKGDLIVIELGDRIPVDGIVVSGSAEIDQSSLTGESIPVNKNTG
                     DKVFSSTLNASGSLVIKAEKVGKDTTFEKLIKLVAESQDNKIGIKTIADKFTSWYIVI
                     TVIGTILLYYFVHDLKMVLAVLLVTCADDIAVAIPMAFSAAIGSAARRGIIVKGGEYF
                     EGLNKVKTILIDKTGTITKGKLKIEGVYSISSATKEKVLKLAAIADCFSEHPVAKAIA
                     DYALGQKVEFEKPSKFKEISGMGSTAIYEKRTVICGREKFLEQSGIKISKEQVAKLEK
                     TKKEGDSILLVGYDNKLVGYIKYGDEIKEDAREAIRRLKELGVEKIVMLTGDNEAVAK
                     KVARKVNVDSFHDNLLPEDKLKYVKEYVGKKNGKVAMIGDGVNDAASLALADVGIAMG
                     VIGTDTAIEAADIALMKDNLLKVPEVFELGKLVTKISHQDFLIWGILNSAGLILAFTR
                     VIGPESAAAFNFVTDFIPIFNSLRLFKYKLSGD"
BASE COUNT          554 a          533 c          432 g          679 t
ORIGIN      
        1 ggtgacatcc acgatcggct ccgtgacggt ttctccgatg aatttcttct cagcttcaga
       61 gggaggcgct gtggcctctt gatttcccag agacgtcaat ttccctcctc caagattatt
      121 ctcccttgtt tgatagttga tgataggtag accaacggca aaaaaagcga gcaggatgaa
      181 cacccattcg atcgtatgca acaacggttc atttttccgg ttgaaaggat gcttgatgaa
      241 atctcccaat ttcttgaaaa atttttttgt tttttgcata atttgtcgcc ccccgacaag
      301 ttgtattttg cttatttata ctgcaattat tataccaaat attcagaaaa gaccaaaatg
      361 aagtttcgtc cctgcttttg ctgcgccaaa cgaaactcaa tctccggaca acttatattt
      421 gaaaagcctt agtgaattga agataggaat aaagtcagtg acgaaattga aagcagcggc
      481 actttccggt ccgatcaccc gggtgaaggc cagaataagc ccggctgaat tgagtatgcc
      541 ccaaatcaga aagtcttgat gagaaatctt ggtgaccaat tttcccaact caaacacctc
      601 gggaactttc agcaaattat ccttcatcaa agctatgtca gccgcttcga tagccgtatc
      661 agttccgatg acgcccatgg caattccgac atcggccaga gcgagagagg ccgcatcatt
      721 cactccatcg ccaatcatcg ccaccttccc gtttttcttt ccgacatatt ctttgacata
      781 tttcaatttg tcctccggca acaggttgtc gtggaatgaa tcaacattga cttttctcgc
      841 tacctttttt gccaccgcct cgttgtctcc cgtaagcata actatttttt ccacccccag
      901 ttctttgagc cggcggatgg cctctcttgc gtcttccttg atttcatcgc cgtattttat
      961 ataccctacc aatttgttat cataaccgac aagcaatatg gaatctccct ctttttttgt
     1021 tttttccagt ttggcaactt gttctttcga tatttttatt cccgattgct ccaaaaattt
     1081 ctctcttccg caaataaccg tccttttttc atatatagct gtgcttccca taccggaaat
     1141 ttccttgaac ttactcggtt tttcaaattc aactttttgt cccagagcat aatcagcaat
     1201 ggctttggcg acgggatgtt cggaaaaaca atcggcaatt gctgccagct tcaggacttt
     1261 ttctttggta gcactgctga ttgaatacac tccttcgatt ttcaattttc ctttggttat
     1321 cgtccctgtt ttatcaatca gaatagtctt cactttattg agcccttcga aatattcccc
     1381 tcccttgacg attatccctc ttctggccgc actgccgatg gcagccgaga aagccatcgg
     1441 aatggcgacg gcaatatcat cggcacaggt gaccagaaga accgccagaa ccattttcaa
     1501 atcgtggaca aaataataaa gcaaaatcgt tccgatgacg gtgataacga tataccagct
     1561 ggtgaacttg tcggcaattg ttttgatgcc tattttgtta tcttgcgact cggcaaccaa
     1621 ttttatcagt ttttcgaaag tcgtatcttt cccaactttt tccgccttga tgaccaaaga
     1681 gcccgaggca ttgagcgtgg agctgaaaac cttgtctccg gtatttttgt tcacgggtat
     1741 cgactcgccg gtcagcgaag actggtcgat ttcagctgac ccgctgacaa cgatgccatc
     1801 caccggaatg cggtctccca attcgatgac aatcaagtcg ccctttttga tctttgaaat
     1861 tgatttttca acaacacgat ttccattttt tatctttacc ctttctggtc tcaatttcag
     1921 aaggcttttg atggcgtttt gggaacgcct ttcggtatag tcgccaaaaa ttctggcgga
     1981 agtcagcatc aggttgatga agaccgctga cgcccattct ttgttgagca gcgaaacaac
     2041 aagagcgatg ctggccaaaa gatcgacgga tatttttttg ttttttaccg atttatacgt
     2101 gctgacgaga acgggcaagg tggcgatgca ggaaaagaaa gtgagaaaaa aatcgtcaaa
     2161 tttttttggc gatatattcg aatagtgacc tctccctt
//
LOCUS       DATKGS010000090         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_153364, whole genome shotgun sequence.
ACCESSION   DATKGS010000090 DATKGS010000000
VERSION     DATKGS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153364"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1206)
                     /locus_tag="VMQ48_02200"
     CDS             complement(4..1206)
                     /locus_tag="VMQ48_02200"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUD08883.1"
                     /translation="MLRGENFRTVARKIHTSQIRLASCLREKPVACFFAGWIRKYAAT
                     VVVAICVLLVALINFSSGNNPNGNIFDTLTGTDSSQSPLKNKLATENYKKNNLAVAPL
                     ANVSAPTSAANVSADYADFYSADDYAAMYQPEPTIDNQILFASIAPQTPTAKEKRGAK
                     TYEVKMGDTASTIAAAFGISTSTILSANSLSDTSLLKPGDKLTILPITGASYKIQSGD
                     NLEAIAKKYNVDNQKIIVYNNLPADGSLKEGQTIVIPDGYLSPVGATQTAGTQLAITG
                     QTQSPLSLSGATSYAPIALGGGGGGHRFPYGYCTWYVAQKRYVPWGGNAISWLSNARA
                     SGKPTGRAPRPGAIMVSGESRWGHVALVESVSGSSFTVSEMNYAGFGKKDFRTISTNS
                     GFVRGFIY"
     gene            1545..2492
                     /gene="xerA"
                     /locus_tag="VMQ48_02205"
     CDS             1545..2492
                     /gene="xerA"
                     /locus_tag="VMQ48_02205"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HUD08884.1"
                     /translation="MDIKQLKTQYLEYLEIEKDRSQKTIENYDHYLERFFAWAKIKLP
                     ADITDNLIRSYRLYLNRMSDEKGKSLKKITQNYHIIAVRNFLKYLAKRDIKALSADKI
                     ELGKTQRMEVEFLDSDEIDRIFEAVAGLDLKSLRDRAIIELLYSSGLRVSELTNLNRD
                     QVNLKTQEFSVRGKGGKIRVVFISDRAKNSLGRYFEKRPDVDPAVFARIGIKRLENPP
                     KADQPRAGKNFDNLRLTPRSIQRIVKHYALKAGIVKDVHPHTLRHSFATDLIANGADI
                     RSVQEMLGHSSVTTTQIYTHVTNKQLKEVHKAFHGKRRK"
BASE COUNT          688 a          591 c          627 g          636 t
ORIGIN      
        1 ttcctaatat ataaaccctc tcacgaaacc actgttcgtg gaaatcgtac gaaaatcttt
       61 ttttccgaaa ccggcgtaat tcatttcgga aacagtgaaa cttgatccac tcaccgattc
      121 aacgagagca acgtgacccc agcgcgattc gcccgaaacc atgatcgctc ccgggcgagg
      181 cgctctgccg gtcggtttgc cggacgcgcg ggcgttcgag agccaagaaa tggcgtttcc
      241 gccccaaggg acataacgtt tttgggcgac ataccaagta caatagccgt acggaaagcg
      301 atggccaccc ccgcccccgc caagggcgat aggcgcataa gaagtcgctc cactcaaaga
      361 gaggggtgat tgggtttgac cggtgatggc caattgtgtc cctgcagttt gagttgcccc
      421 gacgggagaa agatagccat ccggaataac aatagtctgt ccttctttca aggaaccgtc
      481 agccggcagg ttgttgtaga caataatctt ctgattgtcc acgttatact ttttcgcaat
      541 agcctcaaga ttgtcaccac tttggatttt gtagcttgct ccagtgatcg gcagaatggt
      601 gagtttgtcg ccaggcttca acagtgaagt gtccgaaaga ctgttggccg aaagaatggt
      661 gcttgtcgaa attccgaaag cggcggcaat ggtgctggct gtatcaccca tcttcacttc
      721 atatgtcttt gctccccgtt tttctttggc ggtaggtgtt tgaggagcaa ttgaggcgaa
      781 gagtatctgg ttgtcgatag tcggttcagg ctgatacata gccgcatagt catcggcgga
      841 ataaaaatcc gcatagtcgg ccgaaacatt tgcggcgctt gtcggagcgc tgacgtttgc
      901 aagcggcgcg actgccagat tatttttttt gtagttttcc gttgccaact tgtttttcaa
      961 ggggctctgg ctgctgtcgg ttccggttag agtgtcaaaa atattcccat tcggattatt
     1021 tccggaagaa aagttgatta gtgcgacgag taacacgcaa atagctacca cgacggtagc
     1081 tgcatatttc ctgatccatc cggcgaaaaa gcaagcaaca ggcttctctc gcaggcacga
     1141 agccaagcgg atctgggatg tatgaatttt tcgggcaact gttcgaaagt tctcccctct
     1201 tagcaaagtt cgcagttttc gtcccgctcg gtccgttggg cggcgaggaa aatcgttgaa
     1261 atggctacta ataggattgt tccttctttt ggatcagagg ttcgagctga ccggaaaaaa
     1321 ctgatttact gaagcgattt ccgtctgatc cctaattatt acacggctac taggttagca
     1381 tattgccaaa tggtgtcaag gcgcccaaaa catgagagat gtgaaaatcg gattattttg
     1441 gcttatataa ggaaaatatt ggccagtgtg taaattggga ttaatctggt ataattccaa
     1501 atacatgtaa cacaaagcat gtagtatata actaaaaatc agaaatggat attaagcaac
     1561 tcaaaacaca gtatctggaa tatctcgaga tcgaaaaaga ccgttcccaa aaaacgattg
     1621 aaaactacga tcattatctg gagcgctttt tcgcttgggc gaaaatcaag ttgccggcgg
     1681 acattaccga caatctaatt cgcagctatc gcctctacct caaccggatg agtgatgaaa
     1741 aaggaaaatc gctcaaaaaa atcacccaga actatcatat catcgccgtt cgcaattttt
     1801 tgaaatattt ggcgaagcgc gatatcaagg cgctttcggc tgacaagatt gagttgggaa
     1861 aaacgcagcg gatggaagtt gagtttctcg acagcgatga gattgatcga atttttgaag
     1921 cagtggcagg attggatctc aagtcgttgc gcgaccgggc gatcattgag ctgctctata
     1981 gctccggctt gcgtgtttca gaactcacga acctcaaccg tgaccaggtc aatctcaaaa
     2041 cgcaagaatt cagtgtgcgc gggaagggcg gaaagatccg ggtggttttc atttccgacc
     2101 gagccaaaaa ttcacttgga cgatattttg aaaaaagacc ggatgtcgat ccggcggtct
     2161 tcgcgcggat cgggatcaaa cgattggaga atccgcccaa ggcggaccag cctcgggctg
     2221 gaaaaaattt tgataattta cgacttacgc cgcgtagtat ccagcgcatt gtgaaacatt
     2281 atgctctgaa agctggcatc gtgaaagacg tccatccgca cacgctgcgc cattccttcg
     2341 caaccgatct catcgcaaac ggcgccgaca tccgctcggt gcaggaaatg ctcggccact
     2401 catcggtgac cacgacgcag atctataccc acgtgacaaa caaacagctc aaagaagtcc
     2461 acaaggcttt ccatgggaag agaagaaaat agttctattg aagcagattt actatcagag
     2521 caatcatcac gcttttttct tt
//
LOCUS       DATKGS010000091         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_211527, whole genome shotgun sequence.
ACCESSION   DATKGS010000091 DATKGS010000000
VERSION     DATKGS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211527"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VMQ48_02210"
     CDS             complement(<1..706)
                     /locus_tag="VMQ48_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08885.1"
                     /translation="MKINPQMFRGYDLRGIVGTDLNAEIVEHIGKAFGTYLKRQGISG
                     AVVGQDSRATSPEYSSAIIRGMSWAGVDTIDIGMTMVGTFYWSQYHLNRKGGVFITAS
                     HNPAEYNGMKFANDFSETLVSEGMNELRRMVQEDDIEKGEQEGKTEKKDILGAYIEDL
                     SKRLNISKKFRVVVDASCATPGVVAPKILRSFGMDAVESNCDLDPTFPLGTPDPTETA
                     VAERLKAKVLEEKADLP"
     gene            843..1916
                     /locus_tag="VMQ48_02215"
     CDS             843..1916
                     /locus_tag="VMQ48_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HUD08886.1"
                     /translation="MKLVIMAGGKGTRLWPVSRAGKPKQFQCLTGDKTMLQETYLRLR
                     QIADPADIYVATNQEYVSEVEKEILEFPKKNILAEPIGRNTAPSIALAAAVIAAKDEN
                     EIMGVFPADHFIENPEVLLNAIQKGKEFLEKNDEYLLTFGITPSAPETGYGYIKKDGT
                     LEKMGNVEICKAEKFVEKPNYETAQKYLEAGDYFWNSGMFLWKTKTIIEKFKKYVPDD
                     YERLMKIRDAMNSGNYAEVLEKEFPEMDKNSIDYAILENEPDVAVIPLEMPWSDVGSW
                     TALKDTLISNNRENFVRGEHINFDSENLLVYGSDKLITTIGVKDLIIVDTADAILICD
                     RNKSQLVSDVVKKLEADGKIKNL"
BASE COUNT          577 a          551 c          449 g          504 t
ORIGIN      
        1 ggggaagatc ggctttttct tcaagaactt ttgccttcaa acgctcggct accgccgttt
       61 cggttggatc cggcgtccca agcggaaaag tcgggtcgag atcgcaattg ctttccaccg
      121 cgtccatccc gaaagagcgc aaaattttcg gggccaccac tcccggagtg gcgcaactcg
      181 cgtcgaccac gactcggaat tttttgctga tattcaatct cttactaaga tcttcaatat
      241 atgctccgag aatatctttt ttttctgttt ttccctcttg ctctcctttt tcaatgtcat
      301 cctcctggac catgcgacga agttcattca ttccttcact caccagcgtc tcggaaaaat
      361 cgttggcgaa tttcatcccg ttgtattccg ccgggttgtg cgatgccgtg atgaagactc
      421 cgcctttgcg gttgagatga tattgcgacc aatagaacgt ccccaccatc gtcattccga
      481 tatcgatcgt atccaccccc gcccaactca tccctcggat gatcgccgaa gagtattccg
      541 ggctggtcgc ccgcgaatcc tgtcccacca ccgccccgct gattccctgc cgcttgagat
      601 atgtcccaaa ggcttttcca atgtgctcca caatttccgc gttcagatcc gtcccgacaa
      661 ttccccgcag atcgtacccg cgaaacattt ggggatttat tttcataatg acgcatttta
      721 caaattagcc cctctcctct attattatat acggaaatta aacatttatt caaattatcg
      781 attcgtatat tccctcctca tcggcgggca ggcgtacgga ttcgtaattc gtagagaatt
      841 ccatgaaact agtcattatg gctgggggaa aaggaaccag actgtggccg gtgagccgcg
      901 ccggaaaacc caaacagttt caatgcctca ccggagacaa aacgatgctc caggagacgt
      961 atctgcgcct acgccagatc gccgatcccg cggatatata tgtcgcgacg aaccaggaat
     1021 atgtcagcga agtggaaaaa gaaattttgg aattcccaaa aaaaaatatt ctggcggaac
     1081 cgataggaag aaataccgcc ccttcgatcg ctctggccgc ggctgtcatt gctgccaaag
     1141 atgaaaacga aatcatgggc gtttttccgg ccgatcattt cattgaaaat ccggaagtgc
     1201 tcttgaatgc cattcaaaaa ggaaaagaat tcctcgaaaa aaatgatgag tatcttctga
     1261 cttttggcat cacgcctagc gcgcccgaaa cgggatacgg gtatatcaaa aaagacggta
     1321 cgcttgaaaa aatgggcaat gttgaaatct gcaaagccga aaaattcgtc gaaaaaccga
     1381 attatgaaac ggcgcaaaag tatctcgaag ccggcgacta cttctggaac agcgggatgt
     1441 tcctctggaa aacaaaaacg atcattgaaa aattcaaaaa atatgtgccg gatgattatg
     1501 agcgattgat gaaaattcgc gacgccatga attctggaaa ttatgctgag gttctcgaaa
     1561 aagaatttcc ggaaatggat aaaaacagta ttgactatgc cattttggaa aacgaaccgg
     1621 acgtggccgt catcccgctg gagatgccct ggagcgatgt cggctcctgg accgcgctca
     1681 aagacacgct catttccaac aaccgggaaa actttgtccg cggcgagcat atcaacttcg
     1741 acagtgagaa tttgctcgtt tatggctcgg acaagctcat caccaccatc ggtgtgaaag
     1801 acctcatcat cgtcgacacc gccgacgcta tcctcatctg tgaccgcaac aaatcccagc
     1861 tcgtctccga tgtcgtcaaa aaactggaag ccgacgggaa gatcaaaaat ttgtaaaaat
     1921 actttatgtc actcaaagtc ggcacggagc gaacacaatc aaacatcagt atggccaagc
     1981 aagcaccaaa tctataccct ttatcttcac aataaaactg ccgcctcctc gctacgaacg
     2041 gctaattaca gcccgtaatt caaaaggtag ctcctcctcc c
//
LOCUS       DATKGS010000092         5277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_110312, whole genome shotgun sequence.
ACCESSION   DATKGS010000092 DATKGS010000000
VERSION     DATKGS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110312"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /gene="leuS"
                     /locus_tag="VMQ48_02220"
     CDS             <1..2081
                     /gene="leuS"
                     /locus_tag="VMQ48_02220"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HUD08887.1"
                     /translation="WTQWFFLFLYKHGLAYRKKAKVNWCESCKTVLANEQVEDGVCER
                     CKKEVVQKDLEQWFFRITDFAEDLIKDLDKIDWPESTKTAQKNWIGKSEGAIVKFSIS
                     NFQFPNKSQFKNDQRYLDVFTTRLDTIYGCTYCVVAPEHPLIENLKSEIENIKEVEKY
                     IAKAKKKTDLQRTELQKEKTGVELKGVKATNPFTNESVPIFVADYVLSTYGTGAVMAV
                     PAHDERDWEFAKKYKLPIKEVITQNSKLRTQNHGAKPKIVSESFTEDGILINSKKYDD
                     LASEKAREEMVKWLEKNKLGKKTTTYKLRDWLISRQRYWGAPIPMIHCEKCGWQMVPE
                     KDLPVILPKVKDYRPKGTSPLGTNPNFVNTKCPKCRGLAKRELDTMDTFVCSSWYFFR
                     FADPKNKKEFASKNEIKKWLPVDLYIGGAEHTVLHLLYSRFFTKALYKYKQIDFNEPF
                     AKLRHQGIVVAEDSRKMSKSLGNVVNPDEIVKVFGADVLRLYEMFMGPLEDSKSWNTK
                     SIIGLKRFLERVWNIKLKVKSEKLKITVKNSKLESLLHKTIKKVTEDVENLHFNTAIS
                     SMMILVNEMEKENQIPNTSYEILATILSPFAPHIAEELWHELGHKKSIFLEKWPEYDP
                     KLVVEKEANVVVQVNGKVRDQLMVSVDAKEDEIKRLAFSSEKIKKYIPNIDNIRKIIF
                     VPGRLINFVV"
     gene            2125..3564
                     /locus_tag="VMQ48_02225"
     CDS             2125..3564
                     /locus_tag="VMQ48_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HUD08888.1"
                     /translation="MQNKSHPKGTLHTLSEFRREAGIPLVIAKKMVMWGEVKVVKMKD
                     GAVRITEEEVQRIKETFLNPREKVKYYLRVLGPGVITGASDDDPSGIGTYSSVGAMFG
                     FAILWTAAWLLPIMLAVQEACARIGIVANHGLTHVLGKHYNKKIVLGIVILLIIANVV
                     NIGADLGAMAASLKMLTGADLYFGAVFFALLIILMEIFFQYHVYVRVLKWLTLSVFAY
                     IFAGFMSHPNWFEVLKNAFVPTMRFNSNYVFALIAIFGTTITPYLFFWQASEEVEENK
                     MLKVKWNKLAVHTRVREMRTDVGAGMLLANIVFFFIILTTAQALFKNGVTNIGSAEQA
                     ALALKPFAGNMAYLFFALGIIGTGVLAVPILAGSGAYALCEVMKWREGLELKFSRAKG
                     FYLIIAFSIIVGLLINFIGIEPIKALYYSAYLNGIIALPLLIAIMIVGNDKRIMGTET
                     HPGWVKFFGWLAVVFMSTAIVVTIYLALK"
     gene            3602..4075
                     /locus_tag="VMQ48_02230"
     CDS             3602..4075
                     /locus_tag="VMQ48_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUD08889.1"
                     /translation="MDTLIIFIAKYLVFVSAAIAVFYFLKQPRQKQKEILIFAVVLLP
                     LSYIVAKIVSHFYFDPRPFVVGHFTPLLPHAADNGFPSDHTLFGAAVAFAVFHFNKKL
                     GVLLLFFSILIGFFRVLAGVHHISDILGSIIIVGLVYQLATYVNPSSWRIFQKKL"
     gene            4184..5149
                     /locus_tag="VMQ48_02235"
     CDS             4184..5149
                     /locus_tag="VMQ48_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08890.1"
                     /translation="MIQKEYRKNKNAKKYSFIVGFLFLIFMAASSAGAQELTPVSVNS
                     VGVNETLGTPVDEMISAATSGGLSGAQNISALKQDAKKPASESSIASDPSSDPQPTPP
                     TPAFSSDSLKDLESQGGLSSAPEISSADTPAVFNLAVKFFEQVVFKKDVEFASRPMFD
                     KGLDISGQPTFNKDTAGYAIIGKGNQSVEVDFDQAYDSPPVVTATLSLQQYKDPDVRA
                     VAEDLLLVSDVKYIITNVSKKSFEIMMDHKADSDIPFSWHALAVNDPATANKKGEKLK
                     SKIGNDLGPGNGGTAVPAAAGPAAVSQSNTSQAAGNTPNTSANAN"
BASE COUNT         1506 a         1161 c         1265 g         1345 t
ORIGIN      
        1 aatggacgca atggttcttt ttgtttttgt ataagcatgg ccttgcctac cgcaagaaag
       61 cgaaagtgaa ttggtgtgaa tcatgcaaga cggttttggc gaacgaacag gttgaagacg
      121 gcgtctgtga gcgttgtaaa aaagaagtcg tgcaaaaaga tctcgagcag tggtttttcc
      181 gcatcactga ttttgcggaa gatctgatca aggacttgga caaaattgac tggccggaat
      241 caaccaaaac cgcgcagaaa aattggatcg gtaaaagcga gggagctatt gtaaaatttt
      301 caatttccaa ttttcaattt ccaaacaaat cccaatttaa aaatgatcaa agatatttag
      361 acgtatttac tacgagattg gataccatat atggatgtac gtactgcgtg gttgcgccgg
      421 agcatccgtt gattgaaaat ttgaaatccg aaatagaaaa tatcaaagaa gttgaaaaat
      481 atattgcaaa agccaaaaag aaaaccgatc ttcagcgcac ggaattgcaa aaagaaaaaa
      541 ccggcgttga gctgaagggc gtcaaagcca caaatccttt tacaaacgaa tctgttccga
      601 tttttgtcgc cgattatgtt ctttcgacgt atggcaccgg cgcggtgatg gcggttcccg
      661 cccacgatga acgagactgg gagttcgcga agaagtataa gttgccgata aaagaggtaa
      721 taactcaaaa ctcaaaactc agaactcaga accacggcgc aaaaccaaaa attgtcagcg
      781 aatctttcac tgaagacggt attttaataa attctaaaaa atatgatgat ttagcttctg
      841 aaaaggcgcg ggaggaaatg gtgaagtggc tggagaaaaa taagcttggc aaaaagacca
      901 cgacctataa actccgcgat tggctgattt cgcgccagcg gtattggggc gctccgatcc
      961 cgatgatcca ttgcgaaaaa tgcggctggc aaatggtgcc ggaaaaagat ctgccggtga
     1021 ttttgccgaa agtgaaagat tatcggccga aaggaacttc gccgctgggg acgaacccga
     1081 atttcgtcaa tacaaaatgt ccgaagtgcc gaggtttggc gaaacgcgaa ctcgatacga
     1141 tggatacttt tgtttgttca tcctggtatt ttttccggtt tgccgatccg aagaacaaaa
     1201 aagaattcgc aagcaaaaat gaaataaaaa agtggctgcc ggtcgatctc tacatcggcg
     1261 gagcggagca tacggtttta cacttgctct attcgcgttt tttcacgaag gcgctctaca
     1321 aatacaaaca aatcgatttc aacgaaccgt tcgcgaaact gcgccatcag ggaattgttg
     1381 tggcggaaga cagccggaaa atgtccaagt cgcttggcaa cgtcgtcaac ccggatgaga
     1441 ttgtgaaagt tttcggcgcg gacgttttgc gcctttatga aatgttcatg gggccgctcg
     1501 aagatagcaa aagttggaac acgaaaagta ttatcggtct caaaagattt ttggagagag
     1561 tgtggaatat taagttaaaa gttaaaagcg aaaagttaaa aattacagtt aaaaattcaa
     1621 aattagaaag tttattgcac aagacaatca aaaaagtcac ggaagatgtt gaaaatctgc
     1681 atttcaatac ggccatttcg tcgatgatga tcctcgtcaa tgaaatggaa aaagaaaatc
     1741 aaataccaaa taccagctac gaaatactag ctactatttt gtctcctttc gctccgcaca
     1801 tcgctgaaga actttggcac gaacttggac ataaaaagtc cattttcctt gaaaaatggc
     1861 cggaatatga tccaaaactg gtcgttgaaa aagaggccaa tgtcgtcgtt caagtgaacg
     1921 gaaaagtgcg cgatcaactc atggtttcgg tggacgcgaa ggaagatgag atcaagcgct
     1981 tggcgttttc gtcagaaaag atcaaaaaat atattccaaa cattgataac ataagaaaaa
     2041 taattttcgt tccgggacga ctcatcaatt tcgtggttta gcctaacatc cgatgttagg
     2101 tgaagatgaa tgtataatat aaaaatgcaa aacaaatccc atccgaaagg cactttgcac
     2161 accctttccg agtttcggcg cgaggccgga ataccgcttg tgattgcgaa aaaaatggtg
     2221 atgtggggag aggtaaaagt ggtgaagatg aaggacggag cggtgaggat cacagaagaa
     2281 gaagtccaga ggatcaaaga aaccttcctc aacccgcgcg aaaaagtgaa atactatttg
     2341 cgcgtgcttg gtccgggcgt catcaccggc gcgtcggatg acgatccgtc cggcattgga
     2401 acctattcct cggtcggagc gatgttcggt ttcgcgattt tgtggacggc agcttggctt
     2461 ttgccgatca tgctggcggt tcaggaagcg tgtgcccgga ttgggatcgt agccaatcac
     2521 ggcctgaccc atgtcctagg aaagcattac aacaaaaaaa tagtcttggg gatcgtgatt
     2581 ctgctgatca tcgccaatgt ggtcaatatc ggggccgatc tcggagcgat ggcggcttcg
     2641 ctcaaaatgc tcacgggagc cgacctctat ttcggagcgg tcttcttcgc gttgctcatc
     2701 attctcatgg aaatattttt ccaataccat gtttacgtca gagttctcaa gtggctcact
     2761 ctgtcggtct tcgcctatat cttcgccgga ttcatgtcgc atcccaattg gtttgaagtt
     2821 ttaaagaacg cttttgtccc gacgatgcgt ttcaactcaa attatgtctt cgccctcatc
     2881 gccatttttg gaacgaccat cacgccttat cttttcttct ggcaggcttc ggaagaagtt
     2941 gaagaaaaca aaatgctcaa ggtgaaatgg aacaagctgg ccgtgcatac ccgcgtgcgc
     3001 gagatgcgga cagatgtcgg agcggggatg cttctggcca acattgtctt ctttttcatc
     3061 attctcacga cagcgcaggc tcttttcaaa aacggcgtga ccaacatcgg ttcggccgag
     3121 caagcagccc ttgctctcaa gccttttgcc ggaaatatgg cctatttgtt tttcgctctt
     3181 ggcatcatcg gcaccggagt tttggccgtg ccgattttgg ccggctcggg cgcttacgcc
     3241 ctttgtgagg tgatgaaatg gcgcgaaggg ctggaactca aattttcgcg ggccaaagga
     3301 ttctatctca tcatcgcctt ttcgatcatt gtcggactcc tcatcaattt catcggcatt
     3361 gaaccgatca aggcactcta ttattccgct tatctcaacg ggatcattgc gcttccgctc
     3421 ctcattgcta taatgattgt tggcaatgac aagcggatca tggggacgga aacgcatccg
     3481 ggctgggtca aattcttcgg ctggctggcg gtcgtgttta tgtctacagc aatcgttgtg
     3541 acaatatatt tagccttaaa gtaaaacaat atccaagagc ttggttcttg gagaaaatat
     3601 tatggatacg cttatcatct tcatcgccaa atatctggtg ttcgtctcgg cggcgatcgc
     3661 ggttttttat ttcctgaaac agccgcggca gaagcaaaaa gaaatcctta ttttcgcggt
     3721 ggtgctgttg ccgttgtcct atatcgtcgc aaaaatagtt tcccatttct atttcgatcc
     3781 gcggccgttt gtggttgggc atttcacgcc gctccttcca cacgcggcgg acaacgggtt
     3841 tccgtcagac catacgcttt tcggagcggc agtcgctttc gcggtttttc atttcaacaa
     3901 aaaactggga gtgcttctgc tatttttttc cattcttatt ggttttttcc gggttctggc
     3961 cggagtgcat catatttccg acattttggg tagtataatc atagtaggat tggtttatca
     4021 gctcgcaacc tatgtcaatc ccagctcttg gcggattttc caaaaaaagt tataatatcg
     4081 agagcgaaaa aaacaaaaaa aacaaaaaat ttggaaggat tatggccttt ttggcgcctt
     4141 ccttttttat tggccaggaa attgaaatat aaatcaacaa aatatgatac aaaaagaata
     4201 tagaaagaac aaaaatgcaa agaaatactc ttttattgtc ggttttcttt tccttatttt
     4261 catggcggcg tcatcggcag gagcccagga attgactccc gtgagcgtga acagtgttgg
     4321 agtgaatgag acattgggaa caccggttga tgaaatgatc tcagcggcca cttcaggcgg
     4381 cttgtcggga gcccaaaata tttctgcctt gaagcaggac gctaaaaaac cggcatccga
     4441 aagttcgatc gcgtccgatc cgtcgtccga cccgcaacca acaccaccga cacctgcttt
     4501 cagttccgat tccttgaaag acctggaatc gcagggaggc ttgagctccg cccccgagat
     4561 tagcagtgcg gacacgccgg ccgttttcaa tctggccgtc aaattttttg agcaggttgt
     4621 tttcaaaaaa gacgtggaat ttgcgagccg ccccatgttc gacaagggct tggatatttc
     4681 cggccagccg actttcaaca aggacaccgc cggctatgcc atcatcggaa aaggcaacca
     4741 aagcgtcgag gtggatttcg atcaggcata tgattcgccg ccggtggtca cagccacgct
     4801 atcccttcag caatacaaag atcccgacgt gagagcggtg gcggaagatc tgcttttggt
     4861 ctcggacgtg aaatatatca tcaccaatgt gtcgaaaaaa agtttcgaga taatgatgga
     4921 tcacaaggct gattcggaca ttcctttttc ctggcacgca ctcgccgtga atgatcccgc
     4981 aaccgcgaat aaaaaaggag aaaaattgaa gagcaaaatc ggaaatgatc tcggtccggg
     5041 taacggggga acggctgttc ctgctgctgc cggtcccgcc gccgtttccc aatcaaacac
     5101 ttctcaagcg gcaggcaata ctccaaatac tagcgcaaac gccaactaat tttttgaaat
     5161 gaaccatcaa aatgctggac gtaaaaaggg ccaaatacca tccgagcccc aatctcaaga
     5221 tcgcagcaac gcgctggttt gaaagaagat tgatatacaa gggaaaatca tatcccg
//
LOCUS       DATKGS010000093         6728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_211772, whole genome shotgun sequence.
ACCESSION   DATKGS010000093 DATKGS010000000
VERSION     DATKGS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211772"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1893
                     /gene="dnaG"
                     /locus_tag="VMQ48_02240"
     CDS             97..1893
                     /gene="dnaG"
                     /locus_tag="VMQ48_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HUD08891.1"
                     /translation="MASPEIEEIKNRLNIAEVVGEYIRLTKAGSNWKALCPFHNEKSP
                     SFMVSEERGSWHCFGCGKGGDVLSFVMELEGVGFREALEQLAGKAGVELKKYSKGDWP
                     SQETRDDKARLLAILELATKWYEKNLWEGQGKEKILKYLRERGLNDQSIKKFRLGYAP
                     DGWRNMLEFLLKKNYTIEEIAKTGLLVEKESGKEYYDRFRDRIMFPIQDILGRVVGYS
                     ARVAPGGDEKNAKYINTPQTALYDKSKVLYGLNLAKMEIKKRNETVLVEGNTDVIAAH
                     QAGFGNTVAVSGTALTDDQVKILKRYGENINMGFDMDAAGQAAARRSIKTCLENDLNV
                     KIILLSSGKDAAESLKENPEVWKRAVESASGVMEYYFKEIFSRYDPKKPAEKKKIAQE
                     LLNVIKDISNPVEQSHWLKLLASRIGTEEKVLTDILQKSKTSEKNQAISENPEARVNH
                     EKSLEEKILGIILAFPKECRREVVRIDVGDFQNEKERAILDAIKQDLENFSLERVKAL
                     FSDYETGKILEEIVFETEVRFGGELGNGRDQLDPIGELSGYVERLKENRRKEKIFRVM
                     QDIKSAETSGDKAGAKLLMGEFQKLMEEATGE"
     gene            1927..3306
                     /gene="rpoD"
                     /locus_tag="VMQ48_02245"
     CDS             1927..3306
                     /gene="rpoD"
                     /locus_tag="VMQ48_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HUD08892.1"
                     /translation="MPKKKSAQGRSASGGKAKKKSVKKKVQKKPKKKPVKKTVRKKSA
                     KKKIIKKTKKAAPKKVVKKKSASGGKKENSVSRKKEALKKALRAPKKKQENISVEKRG
                     PIDELVYIGKQRGFITEDEILHVIPEVETDVSGLEKLYEKLELSGVKVISSDEVIKTE
                     IEKEAEKMDKKVEHMDVPLDLDDVSSDSVQMYLREIGRVPLLKGEEEVSLAKRNEKGD
                     LMAKQKLTEANLRLVVSIAKKYVGRSANLSLLDLIQEGNIGLFRAVEKFDYRKGYKFS
                     TYATWWIRQAITRALADQSRTIRIPVHMVETINKYTQATRRLVQDLGRDPLPEEVAAE
                     MGLEVEKVRHIMKISQETVSIETPVGDSEDDSILGDFIEDTEAVMPHQNAARTLLTDH
                     VIEVLKDLTPREQKILKIRFGLEDGVTHTLEEVGHEFGVTRERIRQIEAKALDKIRES
                     GLAEKLRDY"
     gene            complement(3409..4266)
                     /locus_tag="VMQ48_02250"
     CDS             complement(3409..4266)
                     /locus_tag="VMQ48_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08893.1"
                     /translation="MSNLVKSTNFGGIYARSLHSENYTTRETKYFRKYQKFTNQLEKR
                     LEIAQNSWKKSKKLLLYIKNNLRSHLSEKQELYWPVLIAITLLLVVFASRIANAATDG
                     TPNNLPLRQAGLPISDNSKLNLIDFEKNAKPAKNTLPAIADAKANDGDLKFDIEKIVK
                     NTPMAAMIDSISEKDRTVAAFIVGIAMKESKFGAYAPHIAGRDCYNYWGLKAGGKTTA
                     GGYSCFSSPEDAVSVVGKTVEKMVAKGVHTPAQAISWKCGSSCAGHDPTGVRKWVADV
                     GVNFYKINS"
     gene            complement(4534..5544)
                     /locus_tag="VMQ48_02255"
     CDS             complement(4534..5544)
                     /locus_tag="VMQ48_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08894.1"
                     /translation="MFCFVDFRNRPQYKYTFHSREKAREYLRKIQNNPLGATVIHADG
                     RREYFASGVPANLLRSGSDFRIEKGCHVKYKIRPLAVMDGIASNRSKGKTSNISNWKS
                     LKSSPLFMLGNLKFSACLLIISITAFSFTYLFQKNFGRSAMAEELKNSGGIVLGATDN
                     QVDDSSAQLTDDSNPLQLADEDIVMNLISKIDDEQHADFTNEILTYVKGRPMEDMAPY
                     IAKQPRIVAAFIVGISMKESKFGVYAPHDANGNDCHNYWGYRGPENTTASGYSCFATP
                     EDAVTAVGRRIARLVDQGASNPSEMVVWKCGASCSWDNPENVRSWIADVGINFYKLNN
                     KS"
     gene            complement(5595..6413)
                     /locus_tag="VMQ48_02260"
     CDS             complement(5595..6413)
                     /locus_tag="VMQ48_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HUD08895.1"
                     /translation="MKRTVKILIWILLIAGAASLLEFFVARKNIKQPLPQAQSNISNQ
                     QQANETNSMASVIADTPTTTNLDPVPASDTAADTAPANPLPGKFLLQVPFYSQAPLGK
                     WDNTHENACEEASVLNAGLYLLGKKLSPSQFDQEIMSMIKIEKKLSINFTDSTIAETK
                     QFSDSYFAGKINSKIIVSPTIEDIEAEITAGNPVIVPLSGRDIGNPNFTPPGPVYHML
                     VVKGYDAQNFITNDVGTRKGDSYTYAKDVIMKNMHDWNAKDIHLGAKIILVLYK"
BASE COUNT         1874 a         1420 c         1769 g         1665 t
ORIGIN      
        1 agggaaagga gtaataatag acaacttggg ggaaattgtg tgaaaaagca aatttatttg
       61 cctattttgg gtattttgct aatatgatag gcagaaatgg cttctccgga aattgaagaa
      121 ataaaaaatc gcctgaatat cgcggaagtg gtgggcgagt atatacggct aacaaaagca
      181 gggagcaact ggaaagctct ctgtcctttt cataatgaaa aatcaccgtc ttttatggtc
      241 agcgaagagc gggggagttg gcactgtttc gggtgtggaa aaggcgggga tgtcctttct
      301 ttcgtgatgg agctcgaggg agtcggattc cgggaagcgc ttgagcaact ggccgggaaa
      361 gcgggagtgg aactcaaaaa atattcaaag ggggattggc cgagccaaga gactcgggac
      421 gacaaagcca ggttgttggc catcttggag ctggcgacga agtggtacga gaaaaatttg
      481 tgggaaggcc agggaaaaga aaaaatactg aaatatttgc gcgagcgggg gctcaatgat
      541 cagagcatca agaaatttag attaggttat gcgccggacg gctggcggaa tatgcttgaa
      601 tttcttttga aaaaaaatta tacaatcgaa gaaatcgcaa aaaccggctt gctggtagaa
      661 aaagaatcag gaaaagaata ttatgaccgc ttcagagacc ggataatgtt ccctatccaa
      721 gatattctcg ggcgagtagt cggctatagt gcccgggtcg cgcccggagg agatgaaaaa
      781 aacgcaaaat atatcaatac gccccaaacg gcgctctatg acaaaagcaa agtcctctat
      841 ggcctcaatt tggccaaaat ggagatcaaa aaaagaaacg aaacggtttt agtggaaggg
      901 aatacggacg ttatcgcggc gcatcaggcg ggattcggga acacggtggc cgtttccggg
      961 acggcgctca ctgatgatca ggtgaaaatt ctcaaacgat acggcgagaa catcaacatg
     1021 ggtttcgata tggatgcggc cgggcaagca gcagcccgcc gaagcatcaa aacctgtctt
     1081 gaaaatgatc tcaacgtgaa aatcatcctg ctgtcatcag gaaaagacgc ggcggagagt
     1141 ctcaaagaaa atcctgaagt ctggaaaagg gcggtcgaaa gtgccagtgg cgtcatggag
     1201 tattatttca aagagatatt ctctcggtat gacccgaaaa agccggccga gaagaaaaaa
     1261 atcgcccagg aactcttgaa tgtgatcaaa gacatttcca atccggttga gcaatcccat
     1321 tggttgaaac tcctggcttc gcgcatcggg accgaagaaa aagtattgac tgatatcttg
     1381 cagaaatcga aaacgagcga aaaaaaccag gcaatctccg aaaatccgga ggcaagagtc
     1441 aatcacgaga aaagcctgga ggaaaagatc ctgggaatca ttttggcttt cccgaaagaa
     1501 tgccgcagag aggttgtccg gatagatgtc ggggacttcc aaaatgagaa agaaagagct
     1561 atactggatg caatcaagca agatctcgaa aatttcagcc ttgaaagagt gaaggctctt
     1621 ttttctgatt atgaaacggg aaaaattctc gaagaaatag tctttgaaac ggaagtgaga
     1681 tttggcggag agctcggcaa cgggagagac cagctggatc ccattggtga acttagcgga
     1741 tatgtcgagc ggctcaagga aaacaggcgc aaggaaaaaa tttttcgcgt catgcaggat
     1801 atcaagtcgg ccgaaacctc cggcgacaaa gcgggggcca agcttttgat gggagaattt
     1861 cagaagctga tggaggaagc gacgggagag tagtttcgaa acagtaaata tgtttttcaa
     1921 ttaattatgc ccaaaaagaa gtcggcccaa ggccgatccg cctcgggcgg aaaagccaaa
     1981 aaaaagtcag tgaagaagaa ggttcagaaa aagccgaaga aaaaaccggt taagaaaact
     2041 gtgcgaaaaa aatctgctaa gaaaaagatc atcaagaaga cgaaaaaggc tgctccgaaa
     2101 aaagtcgtga agaaaaagtc cgcttccggc gggaaaaagg agaactctgt ttccaggaaa
     2161 aaagaagctt tgaaaaaagc tttgcgtgct ccgaagaaaa agcaggagaa tatctctgtt
     2221 gaaaaaaggg gccctatcga cgaattggtg tatatcggaa aacagcgcgg atttatcacg
     2281 gaggatgaaa ttttgcacgt cattccggaa gtggaaacgg atgtttccgg cctcgaaaaa
     2341 ctttatgaaa agttggaact ttcgggcgtc aaagtcatca gctcggacga ggtgatcaag
     2401 acggagatcg aaaaagaagc cgaaaaaatg gacaagaagg tcgagcatat ggatgttccg
     2461 ctcgatttgg atgatgtttc ctcggactcg gtccagatgt atcttcgcga gattggacgg
     2521 gtgcctttgc tcaagggtga agaagaagtg agcctggcta agcgcaatga gaagggagac
     2581 ttgatggcca agcaaaaact cactgaagcc aacctgcggc tcgtggtttc gattgccaag
     2641 aaatatgttg gccgcagcgc caatctgtcg cttttggatc tcattcaaga agggaatatt
     2701 ggtcttttcc gggcggtgga gaaatttgac tatcgaaaag gatacaagtt ttcaacctat
     2761 gcgacctggt ggatccggca ggccatcacg cgggcgctcg ccgaccagtc ccgcacgatt
     2821 cgtattccgg tccatatggt tgaaacgatc aacaaatata cgcaagcgac gcggcggctc
     2881 gtccaagact tgggacgcga tcctttgccg gaagaagtgg cggccgaaat gggactcgag
     2941 gtggaaaagg tgcgccatat catgaaaatt tcacaggaaa ccgtttccat cgagactccc
     3001 gtcggggaca gtgaagatga ttctattctt ggtgatttca tcgaggacac agaggcggtg
     3061 atgccgcacc agaacgcggc gcggacgctg ctcaccgacc acgtgatcga agttttgaag
     3121 gatctcactc cccgcgaaca gaaaatcctg aagatccgct ttggtcttga agacggtgtc
     3181 acgcatactc tcgaagaagt cggacacgaa ttcggcgtca ctcgcgaacg catccgccag
     3241 atcgaagcca aagcgctcga caaaatacgc gaatcaggct tggcggagaa gttgagggat
     3301 tactaaaagc cgcaggagca aggttgcacc ttgctccgca tatttaaata tagaaaccaa
     3361 aaggagcagg gtacaaccct gctcctgctt tttatgttcc attttgtatt acgaatttat
     3421 tttatagaag tttactccca cgtcggcaac ccatttgcgg actccggtcg gatcatgtcc
     3481 ggcgcaggaa gagccgcatt tccaagagat ggcttgagcc ggagtgtgga cgcccttggc
     3541 gaccattttc tcgactgttt ttccaacaac actcacggca tcttcgggag agctgaaaca
     3601 ggaatatccg cccgcggtgg ttttgccgcc ggctttgagt ccccaatagt tgtagcagtc
     3661 ccgtccagca atatgtggag cataggcgcc gaacttgctt tccttcatcg cgattccgac
     3721 aatgaaagcg gcgacagtgc gatctttttc cgaaatcgaa tcaatcatgg cggccattgg
     3781 ggtatttttc acgattttct cgatatcaaa cttcaaatcg ccgtcattgg cttttgcgtc
     3841 cgcgatagcg ggaagggtgt ttttcgccgg cttggcgttt ttttcaaagt caatcaggtt
     3901 caatttcgaa ttgtccgaaa tcggcaggcc tgcctgccgg agaggcaggt tgttcggcgt
     3961 tccgtcggtt gcagcatttg cgatcctgga agcgaatacg acaagcaaga gagtgatggc
     4021 gatcaaaacc ggccaataaa gctcttgttt ttcagataaa tggcttctca ggttattttt
     4081 tatgtaaagt agtaatttct tagacttttt ccagctattt tgggctattt caagccgttt
     4141 ttccagctgg ttagtgaatt tttgatattt gcgaaagtat tttgtttcgc gagttgtgta
     4201 gttttctgag tgtagagatc tggcataaat ccccccaaaa ttagtggatt ttaccaagtt
     4261 actcatcagg tgatgattat ttcttagctt gggctaaaat gaccttatat aagccgaaat
     4321 tagcttgcag cccggtttta atattcttca catattcgac gggcggagca tcaaaaaaag
     4381 gcatatcaca tatacccctt ttgcattgca caagagctat cctagcacac cccaaaaccc
     4441 tgtcaagggt tgacaaaaat ggacgattgt atttaaaaaa ccccgctctt cacaaaggcg
     4501 gggtctcaac gatgcatatc aaaaaccttt ttcctatgac ttgttgttca gcttatagaa
     4561 attgatgccg acatcggcga tccagctgcg gacgttttcg ggattgtccc aggagcagga
     4621 cgcgccgcac ttccagacga ccatctccga cgggttgctg gctccctggt cgacaagcct
     4681 ggcgattctt ctcccgacgg cggtgacagc gtcctcgggc gttgcaaagc aagaatatcc
     4741 ggatgcggtc gtgttttccg gtccgcggta gccccaatag ttgtggcaat cgtttccatt
     4801 ggcgtcgtgc ggggcataga cgccgaattt actttccttc attgagatac cgacgatgaa
     4861 ggcggcgacg atacggggct gcttggcgat gtacggcgcc atatcctcca tcggccttcc
     4921 tttgacatat gtgaggattt cattggtgaa gtcggcatgc tgttcgtcgt caattttgga
     4981 gatgaggttc atgacaatgt cttcatcggc gagctggagg ggattgctat cgtcagtgag
     5041 ttgagcgctt gaatcatcaa cctgattgtc cgtcgctccc aaaacaatcc cgcccgaatt
     5101 tttcagttct tccgccatgg ccgatcgacc aaagtttttt tggaaaagat aggtgaagga
     5161 aaaggcggtg atggagatga tcaaaaggca ggccgagaat ttcagattgc cgagcatgaa
     5221 aagaggagaa gacttgaggc ttttccaatt ggaaatattc gaagtttttc cttttgaacg
     5281 atttgaggcg atgccatcca tgacggcgag cggacgaatc ttgtatttca cgtggcagcc
     5341 cttttcgatc cggaaatcac tacccgatct caataaattg gccggcacgc cactggcaaa
     5401 atattctcgc cggccgtcgg cgtgaatgac tgttgctcca agggggttgt tttgtatttt
     5461 gcggagatac tcccgggcct tttcacggga atggaaagtg tatttgtatt gaggcctgtt
     5521 gcgaaagtcg acgaaacaga acattatgcg tttttatgtt aggtgttagc tcatactatt
     5581 ttatatggaa aaagctattt gtacaatacc aatattattt ttgcaccaag atgtatatct
     5641 ttcgcgttcc agtcatgcat atttttcatg attacgtctt ttgcataagt ataagaatcg
     5701 cctttgcgcg tgccgacgtc gtttgtgatg aaattttggg cgtcatagcc cttcacgacc
     5761 agcatgtgat agaccggacc gggcggagtg aaattggggt tgccgatatc gcgtccggaa
     5821 agcggaacga tgaccggatt tcccgcagtg atttcagctt cgatatcttc aattgtcgga
     5881 ctcactatta tttttgagtt tattttcccg gcgaaatatg aatcggaaaa ttgcttggtc
     5941 tcggcgatcg ttgaatcagt gaaattgatc gacaactttt tctcgatctt gatcatgctc
     6001 ataatctcct ggtcgaattg agatggagaa agttttttac caagcaaata taatcccgcg
     6061 ttcagtacgg acgcttcttc gcaggcattc tcatgagtgt tgtcccattt cccaagcggc
     6121 gcttgagaat agaaggggac ttggagaaga aattttccag gaagggggtt ggccggagct
     6181 gtgtcagccg cagtgtcgga tgcgggaacg ggatcaagat tagttgtagt cggagtatcc
     6241 gcgatgactg aagccattga gttcgtttcg ttagcttgtt gttggtttga aatattgctt
     6301 tgcgcttgcg gaagtggctg tttgatattt ttccgtgcaa caaaaaactc caagagcgac
     6361 gccgccccgg cgatcaggag tatccagatc aaaattttga cggttctttt catagaatca
     6421 attcagtttc actatttatt atactaactt ttcctaaaaa atattgccaa tcttgtttat
     6481 ccgaacggcc gcggcggggt ttttatttga ccaaaaatga ataagcagag tacaattgga
     6541 gccagctctt aaacaatcaa aaataacggc tttatgccga ttacggggag gttccatcat
     6601 gaacgcgagc gagttttgcg ggtattggtg gaaagttttc accagccccg aatttcagga
     6661 aaagcaaccc tggatgcggc ccgtcatgcg atttttcggt ctgcttcctc ccaacgcgtg
     6721 gacgtcca
//
LOCUS       DATKGS010000094         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_121390, whole genome shotgun sequence.
ACCESSION   DATKGS010000094 DATKGS010000000
VERSION     DATKGS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121390"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VMQ48_02265"
     CDS             <1..366
                     /locus_tag="VMQ48_02265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUD08896.1"
                     /translation="SGDLGWSTKDQLVTEIADKAFSMNPGEISQVISSPLGFHIIKLE
                     EKKSDNGQDLVHLRQIFVKIPTFADWLKGEMKKYNVRVFLRDYQWNSDTAQVEFKNPD
                     LKKFEENLDINSQGDPSIF"
     gene            complement(426..501)
                     /locus_tag="VMQ48_02270"
     tRNA            complement(426..501)
                     /locus_tag="VMQ48_02270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Thr,seq:cgt)
     gene            complement(567..637)
                     /locus_tag="VMQ48_02275"
     tRNA            complement(567..637)
                     /locus_tag="VMQ48_02275"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Gly,seq:ccc)
     gene            790..1803
                     /gene="prfB"
                     /locus_tag="VMQ48_02280"
     CDS             790..1803
                     /gene="prfB"
                     /locus_tag="VMQ48_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HUD08897.1"
                     /translation="MPKKKKRIDELEKISTGENFWGDQEKAQKVMQELEQVRDEIKEL
                     SESETEIDNLSEMTSLAAEDEEIKKEIEKQAEKIEKNIEDIEFETLFRGEYDQSNAIL
                     SIHSGTGGTDAQDWAEMLLKMYLRFAERKNWKAKIISESRGQEAGIKSATIEVIGKLA
                     YGYLRAEAGVHRLVRLSPFNAKNLRQTSFALVEVLPEIDKIKEVEIDPSDLRIDTFRS
                     GGAGGQSVNKTDSAVRITHLPTEIVAASQNERSQLQNKEQSMKILKAKLHQQYLENKE
                     KEKKEIKGEHTEVQWGSQIRSYVLHPYKLVKDHRTKFESKNPEEVLDGNVEEFIETYL
                     KSK"
     gene            1805..>2083
                     /locus_tag="VMQ48_02285"
     CDS             1805..>2083
                     /locus_tag="VMQ48_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUD08898.1"
                     /translation="MIEFRNVKKIYTGGINTIALDDISFVIEKEEFVSLVGHSGAGKS
                     TLLKLLYAEEKPTSGEIFFNGEDISNLTPKQLPFYRRRIGTVFQDLSPP"
BASE COUNT          680 a          454 c          484 g          465 t
ORIGIN      
        1 agcggggatc tcggttggtc cacaaaagat cagctggtca cggaaatcgc cgacaaggct
       61 ttttccatga atccgggtga gatcagccag gtaatctcca gtcctttggg tttccatatc
      121 atcaagctcg aagaaaaaaa gagcgataac ggacaggatt tggtgcattt gaggcaaatt
      181 ttcgtaaaaa ttcccacttt tgccgattgg ctgaagggag agatgaaaaa atataatgtc
      241 agggtctttt tgcgggacta ccaatggaat tccgacacgg ctcaagtcga attcaaaaac
      301 ccggatctca agaaatttga ggaaaatctg gacatcaatt cccaaggcga tccgtcgatt
      361 ttttagcttt ttatatttta attagatact caaaaaccgt cccccgtagg gggacggttt
      421 tattttggag ccgatgcgcg ggatcgaacc gcggacctac gcgttacgaa tgcgtcgctc
      481 tgccaactga gctacatcgg catttttgat tgaagcgggc cgccctccgg gcgagcctcg
      541 ctcaaattat tttataaaat aatttgagcg ggatacggga atcgaacccg tgtttcaacc
      601 ttgggaaggt cgtgtattac cactatacta atcccgcctg cttatttact ttcactaaaa
      661 cattatagca atttgccgca atctggcaat agaaaatcgc ttatgctata ataaaatcaa
      721 tgcaggagat catcgcaaaa attgaagaac tccgccaaag aatcgcctcc gtaagcgact
      781 gcctttgacc tgccgaaaaa gaaaaaacga attgacgaac tggagaaaat ttcgactgga
      841 gaaaatttct ggggagacca agaaaaagcg cagaaagtaa tgcaagagtt ggagcaagta
      901 cgtgacgaaa tcaaggagtt gagtgaatct gaaacggaaa ttgacaattt gagcgagatg
      961 accagtctgg cagcggagga tgaggaaatc aagaaagaaa ttgaaaaaca agccgaaaag
     1021 atcgagaaga atattgaaga tatcgaattt gaaaccctgt tccggggaga atatgaccaa
     1081 agcaatgcca ttttgtcgat ccacagcgga acgggaggaa cggatgcgca agattgggct
     1141 gaaatgctgc tcaaaatgta tctccgcttt gccgaaagaa aaaactggaa agcaaaaata
     1201 atttccgaat cacgcggaca agaagcgggg atcaaaagcg caacgattga ggtgattggg
     1261 aagttggcct acggatatct gcgggcggaa gcgggtgtgc atcgtttggt gcgtctttct
     1321 cccttcaatg ccaaaaattt gaggcaaaca tcttttgctc tggttgaagt tttgccggaa
     1381 attgataaaa taaaagaagt tgaaatagac ccatctgatc tgagaattga taccttccga
     1441 tcaggcggag ccggcggcca gtccgtcaac aaaactgaca gcgccgtgcg catcacacat
     1501 cttccgaccg aaattgtcgc tgcctcccaa aacgaacgca gccagctcca gaacaaagag
     1561 caatccatga aaattctcaa agcgaagctg catcaacaat atctcgaaaa caaggaaaaa
     1621 gaaaagaaag aaataaaagg ggagcatacc gaagtccaat ggggaagcca gatccggtcg
     1681 tatgtcctgc atccttacaa gctcgtcaaa gaccaccgga caaaatttga atccaaaaat
     1741 cccgaagaag tgctggatgg aaacgtggaa gagtttatcg aaacatatct caaatcaaaa
     1801 taaaatgatc gaattccgca acgtcaaaaa aatatatacc ggagggatca acaccatcgc
     1861 tctcgacgat atcagctttg tcattgaaaa agaagaattc gtttcccttg tcggccacag
     1921 cggagcggga aaatcgaccc ttctcaagct gctctatgcc gaagaaaaac cgacttcagg
     1981 agaaattttt ttcaacggag aagatatttc aaacctcact cccaaacagt taccatttta
     2041 tcgccgccgg atcggaacgg ttttccagga tctctccccc ccc
//
LOCUS       DATKGS010000095         6126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_154037, whole genome shotgun sequence.
ACCESSION   DATKGS010000095 DATKGS010000000
VERSION     DATKGS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154037"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VMQ48_02290"
     CDS             <1..292
                     /locus_tag="VMQ48_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HUD08899.1"
                     /translation="DGRREDYKREKLEKGIRKALEKRPIDGKKIEEAIDEIEYKLQLD
                     KNCEVSVRTIGKLVLEKLKELDDVAYLRFASVYKGFGSADSFKKEAEKLMET"
     gene            336..2501
                     /locus_tag="VMQ48_02295"
     CDS             336..2501
                     /locus_tag="VMQ48_02295"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HUD08900.1"
                     /translation="MIAVKRGQKMVKKNQGGFQNRVTRVVKRDGRVVKFEQDKITKAV
                     MKAFAETNEGTERDAQKVTDKIVRLLNKNYQKGNVPEIEEIQDLVERVLMILDFEETA
                     KAYILYREQHRKIRESEESLKEAVDLVDKYIKEIDWQVKENANMAYSLQGLNNYISSI
                     VSSKYWLNRVYSKAIRDAHDEGDFHIHDLDKIAAYCCGWDLQDLLRRGFTGVPGKIAC
                     KPPKHLRTAIGQIVNFFYTLQGEVAGAEAFSSFDTLLAPFVRYDGLSFKEVKQCMQEF
                     VFNVNVPTRVGFQTPFTNITMDLVAPKNIAEESVIIGGVPQKEKYGEFQEEMNMINKA
                     FAEVMSAGDATGRVFTFPIPTYNITKNFDWNDENLKPIWEMTAKYGIPYFANFVNSDM
                     NVEDARSMCCRLRLDNRELYKRGGGLFGANPLTGSIGVVTINLPRIGYLSKNKKDFYA
                     RLDRMMDLAKESLETKRKLIEGFIKQGLYPYARFYLDAIYKRRGDYWSNHFSTIGIIG
                     MNEALKNFMDETTATEKGIKFAGEVMEHMREKMLKYQKETDNLYNLEATPGEGTSYRL
                     AIKDRKKYPDMIFANGQVGKSDKIEPYYTNSTQLPVGHTDDFFEALDLQEPLQIKYTG
                     GTVLHGFLGERINDIETTKKLVKTISSKYSLPYFTISPTFSICPSHGYLVGEHELCPK
                     CVVEQKCEVYSRIVGYIRPIQQWNAGKATEFGDRKEFVV"
     gene            2498..3193
                     /locus_tag="VMQ48_02300"
     CDS             2498..3193
                     /locus_tag="VMQ48_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HUD08901.1"
                     /translation="MILGGLQKLTLIDYPGHLAATVFTVGCNFRCPFCHNPELVLLAK
                     YSASNIFEKEFFDFLETRQGKLEGVCITGGEPTIQPDIVDFIRKIKGLGYKVKLDTNG
                     TRPDVLRLLYAEKLLDFVAMDIKSHLDDYDRTTNSKVDIERIRLSVDLIRNSGIDYEF
                     RTTAVPGLHKEADFAGIGKWLEGSKKYVLQAFEDKGKILNPRLLKKTKGKKLDLEKIA
                     KNIEKYFGKVEVR"
     gene            3330..3584
                     /locus_tag="VMQ48_02305"
     CDS             3330..3584
                     /locus_tag="VMQ48_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08902.1"
                     /translation="MFKKLFLFVIVLAIAVAWLGGIFSVSFSPFSLTIQSPEKDFLKK
                     VVKRVEGVVYHEATIHNPPGNMLPDQIDDNVKGEIKKIVN"
     gene            3615..3956
                     /locus_tag="VMQ48_02310"
     CDS             3615..3956
                     /locus_tag="VMQ48_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08903.1"
                     /translation="MLDKLKKHSVKIFVFTINILLAAIVVLIIREKDQARLLENFQKE
                     LPANENAQSSSSLPSFENSVSTEGAQSDSSQANVSETSPSTNNSVVVPNTPAPATTPV
                     SNSSNAKTKTS"
     gene            3978..4916
                     /locus_tag="VMQ48_02315"
     CDS             3978..4916
                     /locus_tag="VMQ48_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUD08904.1"
                     /translation="MKFEINSGFRALGTDINIRIVFKNETEKEKAEGDLEKAKNIFFS
                     KEKIFSRFDFKSELCRINKNTGIWQKATPDMLYLAGRALYYNEISGGLYDPRVIEVLE
                     KIGYDKDFKKKDFSKADLPSPFSAIRKKLADDLKIGDGKIFFATRMDLSGIANGYIVD
                     CAAQYLKRQGWKNFLVDAGGDMNISGTNDEEKKWRIAVEGVPEEKLMLEISGKGIATS
                     GISRKKWQIKGKKFHHLVNPNDPNNFSYEMRTVSVIAQNTENADGRAKTLVLMGKEKG
                     LEFAKENKIAAIFLDYKGNIFVSPEAKKYVYPNIQC"
     gene            4949..5608
                     /locus_tag="VMQ48_02320"
     CDS             4949..5608
                     /locus_tag="VMQ48_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HUD08905.1"
                     /translation="MQNTQIPWDWYVVRASALVGFLLLYISVFMGTVSCLPGIRKYFL
                     RLRSLNFHCWISLQALIFALIHGVALLFHRFISFSLADILIPFHSSYEPLLVALGTIS
                     LYLMIILVATSYARKYISQKFWRTVHFLNIALYIFSIIHAFYLGTDLKSGLLREIFIW
                     ANGTLILLLAYNLIYRIWSKIKKREVICALPSANPYENIRQSDPTCGSQRGNQNFRRR
                     I"
     gene            5535..5762
                     /locus_tag="VMQ48_02325"
     CDS             5535..5762
                     /locus_tag="VMQ48_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HUD08906.1"
                     /translation="MRIYVKVIPRAGRNEVIKISEGEYKVKVVDPPEKGKANERLIGL
                     LAYHFAVPKSSLHIVAGKSARIKIVDVDIEE"
BASE COUNT         1858 a         1232 c         1428 g         1608 t
ORIGIN      
        1 agatggacgc agggaagact acaaaaggga aaaactcgaa aagggaatca ggaaagcgct
       61 cgaaaaaaga ccgatcgacg gaaaaaaaat cgaagaagct attgatgaga tcgaatacaa
      121 acttcagctc gacaagaatt gtgaagtatc agtaaggaca atcggaaaat tggtcctcga
      181 aaaactcaaa gagctggatg acgtggcgta tctccggttt gcgtcggtat acaagggatt
      241 tggcagcgcc gacagcttca aaaaagaagc agagaagttg atggaaacat aaattaaaaa
      301 attcataatt aataaatttc gggggcgaaa acaaaatgat cgcagtcaaa cgcggccaga
      361 agatggtcaa gaaaaatcaa ggggggttcc agaatcgcgt cacgcgggtg gtgaagcgcg
      421 acggacgggt cgtcaaattc gagcaggaca aaatcactaa agccgtgatg aaagcttttg
      481 cggaaacaaa tgaggggaca gagagggatg cccaaaaagt gacggacaag atcgtccggc
      541 ttctcaacaa aaactatcaa aaaggcaacg tgccggaaat tgaagagatc caggaccttg
      601 tggagcgcgt tttgatgatc cttgatttcg aagaaacggc caaagcctat atcctctatc
      661 gcgaacagca cagaaaaatc cgcgaatcgg aagaatcgct caaagaagcg gtggacctgg
      721 tcgacaaata tatcaaagaa attgactggc aagtgaaaga gaatgccaat atggcctatt
      781 ctttgcaggg actgaacaac tatatttctt cgattgtcag ctcgaaatat tggctcaacc
      841 gggtctattc caaagctatt cgcgacgcgc acgacgaagg agactttcac atccacgatc
      901 tcgacaaaat cgccgcctat tgctgcggct gggatctgca ggatcttctg cggcgcggct
      961 tcaccggagt tccgggaaaa attgcctgca aaccgcccaa acatttgcgg accgccattg
     1021 gccaaatcgt gaacttcttt tatactctcc agggagaagt ggccggcgcg gaagcctttt
     1081 caagctttga cactcttctt gcacccttcg tccgatatga cgggctatcc ttcaaagaag
     1141 tgaagcaatg tatgcaggaa tttgttttca atgtgaatgt tcccacgcgg gtcggttttc
     1201 aaacgccttt cacgaacatc acgatggacc ttgtggcgcc gaaaaacatc gccgaagagt
     1261 cagtgatcat cggcggtgtt ccccagaagg aaaaatacgg cgagttccag gaggagatga
     1321 atatgatcaa caaggccttt gccgaagtta tgagtgcggg agacgcgacg ggacgcgttt
     1381 tcacttttcc aattcctacc tacaacatca ccaaaaattt tgattggaat gatgaaaatt
     1441 tgaagccgat ctgggaaatg acggccaaat atggcattcc gtattttgcc aattttgtga
     1501 actcggacat gaatgtggag gatgcccgtt cgatgtgctg ccgtttgcgc ctcgacaacc
     1561 gtgagctcta taaacgtggc ggcggtcttt ttggtgccaa tccgttgact ggatcgatcg
     1621 gggtggtgac catcaatttg ccccgcatcg gctatctttc gaaaaacaaa aaagattttt
     1681 atgcccgtct tgaccgcatg atggatctcg ccaaagaaag ccttgaaacc aaaagaaagc
     1741 tgatcgaagg attcatcaaa cagggccttt atccgtatgc cagattttat ctagacgcga
     1801 tctacaagcg ccgcggcgac tattggtcga accatttttc gacgatcggc attatcggga
     1861 tgaacgaagc gctcaagaat tttatggatg aaacaactgc gacggaaaag ggaatcaagt
     1921 ttgctggaga ggttatggag catatgcgtg aaaaaatgct caaataccaa aaagaaacgg
     1981 acaatctata caatctggaa gcaactccgg gagaaggtac gtcgtatagg cttgccataa
     2041 aagatcgcaa aaaatatccg gatatgattt ttgcgaacgg ccaggtcggg aaaagcgaca
     2101 agattgaacc ttattacacc aattcaaccc agctgcctgt tggccacacc gatgatttct
     2161 ttgaagcgct ggatctgcag gaaccgcttc aaataaagta taccggtggc acggttctcc
     2221 atggatttct gggcgagcgg atcaatgata ttgagacgac caagaagctt gtgaaaacaa
     2281 tttcttcaaa atactctctt ccatatttca ctatttctcc cactttcagc atttgcccgt
     2341 cgcacggcta tcttgtcggc gagcatgagc tctgcccgaa atgcgtggtc gagcagaaat
     2401 gcgaagtcta ctcgcgcatc gtcggctaca tccgtcccat tcagcagtgg aacgccggaa
     2461 aagcgaccga gttcggagat cggaaagagt ttgtggtatg atcctcggcg gtttgcaaaa
     2521 actgactctc attgattatc ccggccattt ggctgcgact gttttcactg tgggttgcaa
     2581 ttttcgttgt ccattttgtc ataacccaga attagtgtta cttgccaaat atagcgcatc
     2641 gaatattttc gaaaaagaat tttttgattt tttggagaca agacagggaa aacttgaggg
     2701 ggtttgcatc acaggcgggg agccgacgat tcagccggat attgtggact tcatccgcaa
     2761 aatcaaggga ctgggataca aagtcaagct ggacacgaac ggcactcgtc cggatgtttt
     2821 gaggctctta tatgcggaaa aactgcttga ttttgtggcg atggacatca aaagtcattt
     2881 ggatgattat gacagaacaa cgaattccaa ggtggatatt gaacgtatta gactaagcgt
     2941 tgatttgatc cgaaatagtg gaattgacta tgaatttcgc acgactgccg tgcccggttt
     3001 gcacaaagaa gcggactttg cgggtattgg gaagtggctg gaggggtcga aaaaatatgt
     3061 cttgcaggcc tttgaagaca agggaaaaat actcaatccg cgtctcttga aaaaaacgaa
     3121 agggaaaaaa ttggatctcg aaaaaatcgc gaaaaatatt gaaaagtatt tcgggaaagt
     3181 ggaggtgaga taattttcaa ttttagaatt tagaaatttt caaacaattt caaaatgatt
     3241 tgatttccaa aacagattca tttagacatt aataaattta gttattgttt gaaaattgaa
     3301 aattttgaaa ttaaataatt ttttggacaa tgttcaagaa actttttctc tttgtcatag
     3361 tccttgcaat tgccgtcgct tggctcggcg ggattttttc ggtgagtttt tctccatttt
     3421 ccttgacaat tcagagtccc gaaaaagatt ttctgaaaaa agtcgtaaaa agagttgaag
     3481 gggttgtcta tcatgaggcc acgatccaca atccgccggg gaatatgctt cctgaccaga
     3541 ttgacgacaa tgtgaaaggc gagatcaaaa aaatagtgaa ctaaatattt ttttaaggtt
     3601 tagccttaaa aatgatgctg gataaactga aaaaacacag cgtgaaaatc tttgttttca
     3661 cgatcaatat tcttctggct gcgattgtcg ttctcattat tcgtgaaaaa gatcaggcga
     3721 gactccttga aaatttccaa aaagaacttc ccgccaacga aaacgcgcaa agcagttcat
     3781 ctctgccaag ttttgaaaac agtgtgagca ctgaaggcgc gcaaagcgat tcgtctcagg
     3841 caaatgtttc agaaacatca ccgtcaacca acaattcagt tgtggttccg aatactcccg
     3901 ctcccgcaac tactccggtc agcaattcat ccaacgccaa gaccaaaact tcttgataaa
     3961 gcgtatatct ctgcactatg aaatttgaaa taaattctgg ttttcgagct ttaggtacgg
     4021 atattaatat tcgtattgtt tttaagaatg agactgaaaa agaaaaggcg gaaggcgatc
     4081 ttgaaaaagc gaaaaacata tttttttcca aagaaaagat attttcccgt ttcgatttca
     4141 aaagcgaact ttgtcggatc aataaaaaca ccggcatatg gcaaaaagca acgcccgata
     4201 tgctttatct tgctggccgg gcgttgtact ataatgagat aagcgggggg ctgtatgatc
     4261 cgcgcgtgat cgaagttctt gagaagatcg gctatgacaa agatttcaaa aaaaaagatt
     4321 tttcgaaagc tgatttgccg tctccatttt ctgcgatcag aaaaaaatta gccgatgatt
     4381 tgaaaattgg tgatggaaaa atattttttg cgacgaggat ggatttgtcc ggcatagcca
     4441 atggatatat cgtcgactgt gccgcccaat atctcaaaag acagggatgg aaaaattttc
     4501 tggtcgatgc gggcggcgat atgaacattt ccggcacgaa tgacgaagaa aaaaaatggc
     4561 ggattgcggt tgagggcgtt cccgaagaga aattgatgct ggaaatttca ggcaagggaa
     4621 ttgcgacctc cggaatcagc cgcaaaaaat ggcagatcaa aggaaaaaaa ttccatcatc
     4681 ttgtgaaccc gaatgatccg aacaattttt cctatgaaat gcgaaccgtt tccgtgatcg
     4741 ctcaaaatac cgaaaatgcc gacgggcgag ccaaaacgct tgtgctgatg ggaaaagaaa
     4801 aggggctgga attcgcgaaa gaaaacaaaa tcgccgctat ttttttggat tataaaggga
     4861 atatctttgt ctcgccggaa gcaaagaaat atgtttatcc caacattcaa tgttagggcg
     4921 gatccaacat tgaatgttgt agcgccttat gcaaaatact caaattccct gggactggta
     4981 cgttgtccgc gcgagcgctc tcgtcggatt tttgctgctc tacatttccg ttttcatggg
     5041 aacggtttcc tgtctgccgg gaatccggaa atattttttg cgtctgcgca gtctcaattt
     5101 tcactgttgg atttctctgc aagcgctcat ctttgccttg attcatggtg tcgctctctt
     5161 gtttcacaga ttcatctcat tcagcctggc agacattctc attcctttcc actcaagcta
     5221 tgaacccttg cttgttgcgc tggggacgat cagcttatat ctgatgatca ttttggttgc
     5281 cacttcctat gcccgaaaat atatttccca gaaattctgg cggacggtcc attttttgaa
     5341 tatcgccctt tatattttct caatcatcca cgccttttat ctcggaacag atctcaaaag
     5401 tggactgctg cgtgaaattt tcatttgggc gaacggaaca ctgattttac tcttggcata
     5461 caatctgatt tatcgcattt ggtcgaaaat aaaaaagcgg gaagtcattt gcgcgcttcc
     5521 ctctgccaat ccatatgaga atatacgtca aagtgatccc acgtgcgggt cgcaacgagg
     5581 taatcaaaat ttccgaagga gaatataaag tgaaagttgt tgatcctccc gagaaaggaa
     5641 aagccaatga aagattgatc gggcttttgg cgtaccattt cgcggtgccc aaaagttctt
     5701 tgcatattgt cgccggaaag tcggcgagaa taaaaatagt agatgttgat atagaggagt
     5761 agcgatcaaa atttcaagga ggggcttcat ggaatggggt tataaaatta ctgttatttt
     5821 gatcgggctt cttatatttc tagtgcatag agaagcccat gttgagcaaa aaaagaaaga
     5881 atcaaaaacg gaggaacacg aagaataata tgcgtacaaa aaattgtgcg gggcaggtcg
     5941 aaaaagacct gcctttttga tataatgatc ttgtgaccaa atccaaaata ttttttgctt
     6001 tgtctttttt ctttttgctc ggcgtttttt tggcgtcttt ttttcagata gaaaaatata
     6061 ttatcttagt tcttgccgtt tcggcaatca ttatatttgt catcaattat aaaaatagaa
     6121 aagccg
//
LOCUS       DATKGS010000096         4331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_121773, whole genome shotgun sequence.
ACCESSION   DATKGS010000096 DATKGS010000000
VERSION     DATKGS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121773"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VMQ48_02330"
     CDS             <1..510
                     /locus_tag="VMQ48_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HUD08907.1"
                     /translation="FAVELRLIADIGLIGLPNAGKSSLLNELTNANAKVANYSFTTLE
                     PNLGVLDDLVIADIPGLIEGASLGKGLGIKFLKHIRRTKILVHCVSAESEDPRRDYKI
                     VRKELKDFAKELAEKKEILLLTKSDLVSKKELKKKIAILKKINPDVFPVSIHDWDSLK
                     VLSEKLLGA"
     gene            complement(529..1317)
                     /locus_tag="VMQ48_02335"
     CDS             complement(529..1317)
                     /locus_tag="VMQ48_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUD08908.1"
                     /translation="MAKIISLVNQKGGVGKTTTAVNLSVSLAQAGKFVLLVDLDPQAN
                     ATSGLGVDPRTIEKSLYHSMVMGEHPGSHIIKLQNFGHSLLPSNRDLAGATVELVNLE
                     NREYRLYNVLRQIRTDYDYIIIDSPPSLGPLTINGLVASDEVLIPVQCEYYALEGLSQ
                     LLETINLVKDNLQPDLKVMGAVLTMFDRRNRLSYQVVREVKDHFPGKVFDSIIPRSVR
                     LAEAPSFGKSIFEFDLFSKGAKSYKNLAQEVLRMEKEGESGFHL"
     gene            1388..1471
                     /locus_tag="VMQ48_02340"
     tRNA            1388..1471
                     /locus_tag="VMQ48_02340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1422..1424,aa:Leu,seq:caa)
     gene            1537..2361
                     /locus_tag="VMQ48_02345"
     CDS             1537..2361
                     /locus_tag="VMQ48_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HUD08909.1"
                     /translation="MIGVLEVEIKVSKKGHGTMSLTDFENLGFGINDSSLKRALRQVR
                     SLPAIYWQAIANRLAFLKNCEIDPRQVFCPSLGLTDNIVFISQSNKSRFKGNGLYLKS
                     RFKCDAVITDVPGLAIMFLPADCPVIVLFDQKQGILAQVHSGRSNILKNIAGKTARVM
                     KEDFLCNPSDIIAYFSPHLCAECHILTYLEFLNNPEYEEIASAIVEVKGGWQFDMRRA
                     IEIQLGLEGIDRIISEPPACTLCGKEKFFSHRGWEQLHPNHREPGRFVVVSRMVKL"
     gene            complement(2417..3385)
                     /locus_tag="VMQ48_02350"
     CDS             complement(2417..3385)
                     /locus_tag="VMQ48_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08910.1"
                     /translation="MKLMREKNESKKIPARALVLFSGGLDSILAVKILEGQGIEVTAL
                     TFTSYFFDAQQAEKSARENKIKLKIVYFSDAHFDIIKKPKYGRGAGMNPCIDCHLLML
                     VQAKKIAKKENFDIIATGEVLGQRPMSQNFRALELIERKAGLVGKILRPLCAKALPET
                     EMERKQKSPRAKLLVDRTKLYGISGRSRKNQIELAKKYKIKYFPSPAGGCILTEKEYS
                     KKLAELIEKKKKIVSSDLQLLRVGRHFWIEKTRIILGRNHEENLALKKIAEKNDVLIE
                     PEDVPGPTALIRGKKNKAVLDYTKKLLLKYTKNAPDDLQVKIISRK"
     gene            complement(3382..3888)
                     /locus_tag="VMQ48_02355"
     CDS             complement(3382..3888)
                     /locus_tag="VMQ48_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08911.1"
                     /translation="MLDVKKVKYHPSPNLKVRTTRWFERRLIYKGKSYPVKNLLLLAA
                     ALLLILFSAAHFLLAYRNNQADPSLAIQKETKELTDRIGKFMELPTGEQPTLATVTDQ
                     DKLKGQDFFAHAQNGDKLLVYPVARKAILYRPSTGKVIEVSNLVSGGAGQSQPSTDTV
                     PSTDNSSQ"
     gene            complement(3911..>4331)
                     /locus_tag="VMQ48_02360"
     CDS             complement(3911..>4331)
                     /locus_tag="VMQ48_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08912.1"
                     /translation="GSNQSVEVDFDQEYDSPPVVTATLSLQQYKDPDVRAVAEDLLLV
                     SDVKYIITNVSKKSFEIMMDRKADSDIPFSWHALAVNDPVTAKKKGEKLKSKIGNDLS
                     PGSTGATVPAAAGPAAVTQSNTSQAVGDTSNASANAN"
BASE COUNT         1086 a         1013 c          996 g         1236 t
ORIGIN      
        1 tttgcggtcg aactcagatt gatcgccgat attggtttga ttggtttgcc gaatgccggc
       61 aagtcaagcc ttctcaatga actcaccaac gccaatgcga aagtggcaaa ttattctttc
      121 accacgcttg agccgaatct cggagtactt gatgatttgg tcattgccga tatccccgga
      181 ctcatcgaag gcgcctcgct cggaaaaggt ttgggaatca aattcttgaa acatattcgc
      241 cgcaccaaaa ttctggttca ttgcgtttct gcggaatcgg aagatccgag gagagattat
      301 aagatcgtcc ggaaagagct caaagatttt gcgaaagaat tggccgaaaa aaaagaaatc
      361 cttctcctca cgaagtcgga tctggtttca aaaaaagaac tcaagaaaaa aatagccatt
      421 ctaaaaaaaa taaatccgga cgtttttccg gtttcgattc acgattggga tagtttgaaa
      481 gttttgagcg aaaaattgct gggtgcatag aagcctctat ggaagcttct atagatgaaa
      541 tccgctttct ccttcttttt ccatccgcaa aacttcttgc gcgaggtttt tgtatgattt
      601 cgcgcctttt gaaaagagat caaattcgaa aatagatttt ccgaaactgg gcgcttcggc
      661 gaggcggaca ctgcggggaa tgatggaatc aaaaactttt ccgggaaaat gatctttcac
      721 ttcacgaacg acttgatagg acaagcgatt gcgccggtcg aacatcgtga gcaccgcgcc
      781 catcactttg agatcgggct gcaaattgtc tttcacaaga ttgatcgttt cgaggagctg
      841 gctcaaacct tcgagggcgt aatactcgca ctggacggga atgagcactt cgtccgacgc
      901 caccagaccg ttgatcgtga gcgggccaag actgggcggg ctgtcgatga tgatataatc
      961 atagtccgtg cggatttggc gaaggacatt gtataacctg tattcccggt tctcaagatt
     1021 caccagttcg accgtcgctc cggccagatc ccggtttgac ggcagcaaac tgtgtccgaa
     1081 attttggagc ttgatgatgt gcgacccggg gtgctcgccc ataaccatac tgtgatagag
     1141 gcttttttcg atcgtccgcg gatccacccc gagaccgctc gtggcgttgg cctgcggatc
     1201 aagatcaaca agcagcacga acttccccgc ttgcgcgagc gagacagaca aattgacagc
     1261 cgtagtggtt tttcccaccc cgcctttttg gttcacaaga gaaataattt tggccataac
     1321 aaagtaaatt ataccatatt gtttgacata cgactactat ttatttagag tagtataagc
     1381 cagaattgcc tcggtggcgg aactggcaga cgcactggat tcaaaatcca gcgatggcaa
     1441 catcatgcga gttcaactct cgcccgaggc acaaataggc gcaaaagcgc ttattgaatt
     1501 tttataccta ccaaatcaaa caaggaggca cgcgccatga ttggagttct cgaagtcgag
     1561 atcaaagttt ccaagaaagg tcatggcact atgagcctga ccgatttcga aaacctggga
     1621 ttcgggatca acgactcttc cttaaaaaga gcattgcggc aagtgagatc tcttccggcg
     1681 atctattggc aggcaatcgc caaccggctt gcttttttga aaaattgcga aatcgatcca
     1741 aggcaggttt tctgcccatc attgggcttg accgataata ttgtttttat cagccaatca
     1801 aacaaaagcc gtttcaaagg aaacggatta tatctcaaaa gccgcttcaa atgcgatgcg
     1861 gtcatcactg acgttcctgg cctggcaatt atgtttctgc cggctgattg cccggtcatt
     1921 gttctctttg accagaagca gggaattctg gctcaagtgc acagcgggcg aagcaatatt
     1981 ctcaaaaata ttgccggaaa aaccgcccgt gtgatgaagg aagatttttt gtgcaatccc
     2041 tctgacatta tcgcatattt ttcccctcat ctttgcgccg agtgccacat tctcacctat
     2101 cttgaatttc tcaataatcc tgaatatgaa gagatagcgt cagcaattgt ggaagtcaaa
     2161 ggtggctggc aattcgacat gcgaagagct attgaaatcc aacttggcct ggagggaatt
     2221 gaccggataa tctccgaacc gcctgcctgc accctttgcg gcaaagaaaa gttcttttct
     2281 catcggggtt gggagcaact gcatccaaat caccgcgaac caggaaggtt cgtcgtcgtc
     2341 tcgaggatgg tgaaattgta gtcaatcaag gcagggttct gaagaccttg ccttttttct
     2401 ttttcaagta ttttttttat tttcttgaaa ttattttcac ttgaagatca tcaggcgcgt
     2461 ttttcgtgta tttcaagaga agtttttttg tataatcaag aactgctttg tttttctttc
     2521 cccggatcaa agctgtcggg ccaggaacat cttctggctc aatcaagacg tcattttttt
     2581 cagctatttt tttgagcgcc agattctctt cgtggttgcg gccaaggata attcgggttt
     2641 tttcgatcca aaaatgccgg ccaactcgca ggagctgaag gtcgcttgag actatttttt
     2701 tctttttttc aatcagctcg gcgagttttt ttgaatactc tttttcagtg agaatacaac
     2761 cgcccgccgg cgaaggaaaa tattttatct tgtatttttt tgcaagctct atttggtttt
     2821 ttcggcttct tccagaaata ccatataatt tcgttctgtc aaccaagagc ttagctcttg
     2881 gagatttttg ctttctttcc atttcagttt ctgggagagc tttggcgcaa agtggacgca
     2941 gaatttttcc aaccagcccc gcttttctct cgatcagctc gagcgcgcga aaattttgcg
     3001 acattggccg ctggcccagc acttcgcctg ttgcaatgat atcgaaattt tctttttttg
     3061 cgattttttt ggcttgtaca agcatcaaaa gatggcagtc aatgcacgga ttcatccccg
     3121 ctccccgacc atatttcggt ttttttatga tatcaaaatg cgcgtctgaa aaataaacta
     3181 ttttcaattt tattttattt tcccgcgccg atttttccgc ctgctgggca tcaaaaaaat
     3241 agcttgtgaa agtgagcgcc gtcacctcga tcccctgccc ttccaaaatt tttaccgcta
     3301 ggatcgaatc aaggccgcct gaaaaaagca ccagggcgcg cgcaggaatc ttttttgatt
     3361 catttttttc tctcatcaat ttcattggga agaattgtca gtgcttggaa ccgtatctgt
     3421 cgatggctgg ctctgcccgg cgccgcccga gaccaaattg gaaacttcaa tgaccttccc
     3481 ggtggagggc cggtagagaa tcgcttttct ggcaacggga tagaccagaa gtttgtcccc
     3541 gttttgggca tgcgcgaaaa aatcctgccc cttgagtttg tcctgatcgg tgacagtcgc
     3601 caaggtcggc tgctccccgg tcggaagctc cataaatttc ccaattcgat cggtcaattc
     3661 ttttgtttct ttttggatcg cgagacttgg atcggcctga ttgttccgat aagcaaggag
     3721 aaaatgggca gccgaaaaaa gaataagcag aagcgctgcg gcaagcaaaa gaagattttt
     3781 gacgggatat gattttccct tgtatatcaa tcttctttca aaccagcgcg ttgtcctgac
     3841 cttgagattg gggctgggat gatatttgac tttttttacg tccagcattt tgatggttta
     3901 ttccaaaaaa ttagttggcg ttcgcgctgg catttgaagt atcgcctacc gcctgagaag
     3961 tgtttgattg ggtaacggcg gcgggaccgg cagcagccgg aaccgttgct ccagtgctac
     4021 ccggactgag atcatttccg atcttgcttt tcagcttttc ccctttcttt ttcgctgtga
     4081 ccgggtcatt cacggcaagc gcgtgccagg aaaaaggaat atccgaatca gccttgcgat
     4141 ccatcattat ctcaaaactt tttttcgaaa cattcgtgat gatatatttc acatctgaaa
     4201 ccagaagcag gtcttccgcc accgccctca catcgggatc tttgtattgc tggagcgaca
     4261 gcgtggccgt gaccaccggc ggcgaatcat attcttggtc aaaatcaact tcgacgctct
     4321 gattgctccc c
//
LOCUS       DATKGS010000097         5438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_212790, whole genome shotgun sequence.
ACCESSION   DATKGS010000097 DATKGS010000000
VERSION     DATKGS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212790"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VMQ48_02365"
     CDS             <1..295
                     /locus_tag="VMQ48_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08913.1"
                     /translation="GGDPVKHPKVDPQSVWKKDHREVASSDLIIAYVGSPSLGVGAEL
                     EIARITASDLILWWFKGEKVSRMALGNPAVSHRMEVEDEEDLKNKLREILKKY"
     gene            312..998
                     /locus_tag="VMQ48_02370"
     CDS             312..998
                     /locus_tag="VMQ48_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08914.1"
                     /translation="MKKIKRGKFIVIDGTDGSGKATQTELLVKRFKKEGYKVKELDFP
                     QYGKKSAGLVEEYLNGNYGTARDVGPYRASIFYACDRYAASFQVKTWLSEGNIVISNR
                     YVTANMGHQGGKIKNKRERDKYFRWLYDLEYNIFSIPKPDINLILHVDAAIAQKLVDS
                     KEKRDYIKNGKRDIHENDLKHLQDAEKVYLEIAQGFSGFQLIECTKGGFIMSKKEISD
                     LVWNRLKNKL"
     gene            1002..1331
                     /locus_tag="VMQ48_02375"
     CDS             1002..1331
                     /locus_tag="VMQ48_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HUD08915.1"
                     /translation="MNFDEYQEKSRKTAKYPNAGKDWVYPMLGLCGEAGEVAEKMKKV
                     LRDCGGKIDETKKNEIKKELGDVLWYVAQLATELNLSLDEIANGNIEKLYSRMERNKL
                     NGNGDDR"
     gene            1342..2778
                     /gene="gatB"
                     /locus_tag="VMQ48_02380"
     CDS             1342..2778
                     /gene="gatB"
                     /locus_tag="VMQ48_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HUD08916.1"
                     /translation="MKYKPTIGMEVHVELATGSKMFCECKNELGLDRIPNTNICPICT
                     GQPGALPAANEKAIEYVVRAGLALGCEIAKVSKFDRKNYFYPDLPKGYQISQYDQPLC
                     LGGHLDIGGKKIGITRIHLEEDTGKLVHQKGSDFSLVDFNRSGVPLMELVTEPDITSS
                     EEAKKFCEKLQLILRYIGITEADMEKGQMRCEANISVSTNDKLGTKVEVKNLNSFKVV
                     EKAIEYEIKRQAELLDEGKDIAQETRGWDEGRMVTFSQREKESAHDYRYFPEPDLPTL
                     EIGEDFVEKLRAELPELPAQKAKRFVGEYKISEYDAEVLVRDKKLAEFFEESVSEAME
                     SAGSKDRIAKLAANYILAEIRKYLDEKTNVSDLKLTPENFGELMRIIVEGKINSSAAQ
                     IVLSEMIETGADPEQIIAEKNLVQLEDEGEIEDIVKNIISDNSGPVADYKVGKQNALQ
                     FLVGQTMKETKGRVNPQRAEELLKKYLT"
     gene            complement(3174..4148)
                     /gene="miaA"
                     /locus_tag="VMQ48_02385"
     CDS             complement(3174..4148)
                     /gene="miaA"
                     /locus_tag="VMQ48_02385"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HUD08917.1"
                     /translation="MNKILVILGPTSSGKSDIAIKMAKKFNGEIISADSRQIFRGMDI
                     GTGKVEGRLVEVGPQQAAVGGPTSTRGMTKKIFMSQDIPHYMIDIVSPRTDFNVAKFK
                     KQSEKIITDILNRGKLPIICGGTGFWIQAIVDDVNFPEVKPDWKLRERLEKHSPEKLF
                     LMLKKLDRERSQNIDSKNKVRLIRAIEICKKIGKVPSIKNTKYQIRNTKYKTLQIGLK
                     LSREKLYANIEKRVLARFKQGMTKEVENLHTQTGLSWKKIQSFGLAYFWIPLYLQNKI
                     SKKELLEKVIQVEKNYAKRQMTWFNRDKRIKWLENYKKIEKSAKYFLR"
     gene            complement(4145..>5438)
                     /locus_tag="VMQ48_02390"
     CDS             complement(4145..>5438)
                     /locus_tag="VMQ48_02390"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HUD08918.1"
                     /translation="GEKSDAERIATFLEKYKIKPASSIDAADFVIFVTCGVRQSAEDR
                     VYGQINNIKKKSPEKKAILTGCLAHRKDVQRRLKDKVDLYFPISDFQTFENWLIENYL
                     EIGNWKLEIPAQKKYSQQCNSYLSIDPKHTNAFQAFVPVMTGCNNFCAYCVVPYARGR
                     EYSRPAEDILKEVKDLIGKGYKEIMLLGQNVNSYKSPKTNFPKLLHLIEKIPGNFWLT
                     FLTSHPKDMSDELIGTIVKSKKICEYISLPAQSGDNEILLKMNRKYSVEHYKSLIKKI
                     RAAYKKYQPDIFPSISTDIIVGFPSETKKQFENSAKLLREMKFDMAYIAQYSPRPETA
                     AWRLKDSVSKKEKARREKVLTDILSKTALENNKKYVGKIVEVLVDKRITSSPQKYIAH
                     TRTQKKVIFLYNKKITVGSFAKVKITKAKAFGLEGEIS"
BASE COUNT         1607 a          955 c         1304 g         1572 t
ORIGIN      
        1 gggaggtgat ccagtgaaac atccgaaagt cgatcctcaa agtgtttgga aaaaagatca
       61 tagagaggtt gcatcttccg atttaatcat tgcttatgta ggatctcctt cattgggtgt
      121 tggcgcagaa ctagaaattg cgagaatcac agcatcggat cttattcttt ggtggttcaa
      181 gggagaaaaa gttagccgga tggctttggg aaatccggct gtttcgcacc gaatggaggt
      241 cgaagacgaa gaggatttaa aaaataaatt gcgagaaatt ctcaaaaaat actgatttat
      301 tgcaacacct tgtgaaaaaa attaaaagag gaaaatttat tgtcattgac ggaactgacg
      361 gaagcggaaa agctactcaa actgaattgt tggtcaaaag atttaaaaaa gaaggttata
      421 aagtcaaaga attagatttt cctcaatacg ggaaaaaatc cgccggactt gtggaggaat
      481 atctgaatgg aaattatggt acggcccggg atgttggccc ttatcgagct tctatttttt
      541 atgcatgtga tcgatatgct gcgagtttcc aggtaaagac atggctttct gaaggaaata
      601 tagtgatttc caaccgatac gtgacggcca atatgggcca tcaaggagga aaaataaaaa
      661 ataaaagaga aagagataaa tatttcagat ggctatatga tttggaatat aatatttttt
      721 ctatccccaa gccagacata aatttgatac ttcatgttga tgcggctatc gctcaaaagt
      781 tggttgattc aaaagaaaaa cgagattata taaaaaatgg aaaaagagat attcatgaga
      841 atgacctaaa acatcttcag gatgcggaaa aagtatattt ggaaatagcc caagggtttt
      901 ccggttttca gcttatagaa tgcacgaaag gcgggttcat aatgagtaaa aaagaaataa
      961 gcgatttggt ttggaatagg ttaaaaaata aattatagcc aatgaatttt gacgaatatc
     1021 aagaaaaatc gagaaagact gcaaaatatc cgaatgcggg gaaagactgg gtttatccta
     1081 tgctggggct ttgcggagaa gcgggcgaag tggcagaaaa aatgaaaaaa gttctgcgtg
     1141 actgtggtgg aaaaattgat gaaacgaaaa aaaatgaaat caagaaagaa ttgggggatg
     1201 tgctttggta cgttgcccaa ttggcgactg aattgaattt atctctggat gaaattgcca
     1261 atggaaatat tgaaaaatta tacagcagaa tggaaagaaa taaattaaac ggaaacggcg
     1321 acgacagatg aaaataatta catgaaatac aagccaacaa tcgggatgga ggtgcatgtg
     1381 gagctcgcga cgggatcgaa gatgttttgt gagtgcaaaa acgagctggg acttgaccgg
     1441 attccaaaca cgaatatctg cccgatttgc acggggcaac cgggtgcgct tccggcggcg
     1501 aacgaaaagg caattgaata tgttgtgcgg gcggggctcg ccctcggttg cgagatcgcg
     1561 aaagtttcaa agttcgaccg caagaactat ttttatcccg atctgccgaa gggctatcaa
     1621 atttcccaat atgaccagcc cttgtgcctg ggcggccatc tcgatatcgg tggaaaaaaa
     1681 atcggcatca cgcggatcca cctcgaagaa gatacgggaa agctggttca tcagaaagga
     1741 agcgatttca gtttggtgga tttcaaccgc tcgggcgtgc ctttgatgga actggtgacc
     1801 gagccggata ttacatcgtc tgaagaagcg aaaaaattct gcgaaaagct gcagctcatc
     1861 ttgcgctata tcggaataac ggaggcggat atggaaaaag gtcagatgag atgcgaagcc
     1921 aatatttcag tttcgacaaa tgacaagctg ggcacgaaag tcgaagtgaa aaatctcaac
     1981 tctttcaaag ttgttgagaa agcgatcgaa tatgagatca aaagacaagc ggaacttttg
     2041 gatgagggga aagatatcgc gcaggaaacg cgcggctggg atgagggacg aatggtcact
     2101 ttctcgcagc gagaaaaaga atcggcgcac gattatcgat attttccgga gcctgatctg
     2161 ccaacgcttg aaatcggtga agattttgtc gaaaagttga gagccgaact tccggaattg
     2221 ccggcgcaaa aagcgaaaag atttgttggg gaatacaaaa tatcggaata cgatgctgaa
     2281 gttttggtgc gtgacaagaa acttgcggaa ttttttgaag agtcggtgag cgaagcaatg
     2341 gaatcagccg gaagcaaaga caggatcgcg aaattggccg ccaactatat tctcgctgag
     2401 atcagaaaat atctggatga aaaaaccaat gttagtgatt tgaaattgac ccctgaaaat
     2461 tttggcgagc tgatgcgtat aatagtcgaa gggaaaataa attcctccgc ggcgcagatc
     2521 gtactttctg aaatgatcga aacaggtgcc gatccggagc agatcatcgc cgaaaaaaat
     2581 ttggtgcaat tggaagatga aggcgagatt gaagacatag taaaaaatat tatttccgat
     2641 aattccggtc cggttgcgga ctataaagtt ggaaaacaaa atgccctcca atttcttgtg
     2701 gggcaaacaa tgaaagaaac aaaagggaga gtgaatccgc aaagggcgga ggaactgctc
     2761 aaaaaatatt tgacgtagta ctttcaaaag ggggtagcct atgtctgata atccaatgtg
     2821 cagtgggtgt cccctagaaa aggatgccaa ggcgtgtttg attgttgatt gcggagcata
     2881 ttctactatt gagtattgcg gaatacctgc aatatgtctg gatctgatat gctgggaagt
     2941 aaaaaagtgc gattttaggg ggaatattca ggagtgccct gaattgccag aatggagaaa
     3001 agaaaatcct ggcaggtgtg aaatttgcgg ggctatttgc ggacatcact gggacaaatg
     3061 tccaaaataa tgagagggca atttcatgaa tctcacctct tgtcagtttt atggaagatt
     3121 aaagggctgg aaatttccag ccctgctttt tttataaaat aactaagcga tacttatctc
     3181 aggaaatatt ttgcggattt ttctatcttt ttataatttt cgagccattt gattctttta
     3241 tctcgattga accaagtcat ttgccgtttg gcataatttt tttcgacctg gataactttt
     3301 tcgaggagtt cttttttgga aattttattt tgcaaatata gcggaatcca gaaataggcg
     3361 aggccaaaag attgaatttt tttccatgat aatccggttt gggtatgcaa attttccact
     3421 tccttggtca ttccttgctt gaagcgggcg aggacgcgtt tttctatatt agcataaagt
     3481 ttttcccggg atagcttgag accgatttgt aatgttttgt atttggtatt tcgtatctgg
     3541 tattttgtat tttttatcga gggaactttg cctatttttt tacagatctc aattgcccgg
     3601 attaggcgga ctttgttttt ggagtcaata ttttgcgatc tttcacgatc taattttttc
     3661 agcatcaaaa acaatttctc tggagaatgt ttttcaagtc tctcgcgcag tttccaatca
     3721 ggtttcactt cgggaaaatt gacatcgtcg acaatggcct ggatccaaaa gcctgtgccg
     3781 ccgcagatga tggggagctt accccggttt aaaatatccg taattatttt ttcggattgt
     3841 tttttgaact ttgcgacgtt gaaatcggtg cgaggagaaa cgatatcgat catatagtgg
     3901 gggatgtctt gagacataaa gatttttttt gtcataccgc gcgtagaggt cggaccgccg
     3961 accgcagcct gctgcggtcc gacctctact aatcggcctt ctacttttcc tgtccctatg
     4021 tccatcccgc gaaaaatctg ccggctgtca gccgaaataa tttcaccgtt gaactttttg
     4081 gccattttaa tggcaatatc ggatttaccc gaggaagtcg ggccaagaat gacgaggatt
     4141 ttgttcatga tatttctcct tccaatccaa acgctttggc tttggtgatt tttactttcg
     4201 cgaaacttcc aacggtgatt tttttgttgt ataaaaatat aactttcttt tgggtgcggg
     4261 tgtgggcgat atatttttga ggggaactgg ttattctctt atcaactaaa acctcaacaa
     4321 ttttcccgac atattttttg ttattttcca gcgcggtttt ggaaagaata tcggtcagaa
     4381 ctttttcgcg tctggccttt tctttctttg agacactgtc tttcaagcgc caggcggcgg
     4441 tttcggggcg gggggagtat tgggcgatat aggccatatc gaatttcatc tcgcgaagaa
     4501 gcttggctga attttcaaac tgttttttcg tttcgctcgg gaagccgacg atgatgtcgg
     4561 tagaaataga tggaaatata tctggttggt attttttgta tgctgcgcgg atttttttga
     4621 taagactctt gtaatgttcg actgaatatt tgcggttcat ttttagcaaa atttcattgt
     4681 cgcctgattg cgcggggagg gaaatatatt cgcagatctt ttttgatttg acaatcgttc
     4741 caatcaactc atcggacata tctttcggat gcgaagtgag gaaagtgagc cagaaatttc
     4801 cggggatttt ttcgatgagg tggagaagtt ttgggaagtt tgttttggga gacttgtatg
     4861 aattcacgtt ttgtccaaga agcataattt ctttgtatcc ttttccaatc aagtctttca
     4921 cttctttcag aatatcttcc gccggtcttg aatactccct tccacgtgca tagggaacaa
     4981 cgcagtaggc gcagaaattg ttgcagccgg tcataaccgg cacaaaggcc tgaaaagcat
     5041 tggtatgctt tggatcaatt gaaagataag aattgcattg ctgagaatat tttttttgtg
     5101 ccggaatttc caatttccaa tttccaattt ccaaataatt ttcaattagc caattttcaa
     5161 atgtctgaaa atcgcttata ggaaaatata agtcaacctt atctttcagt cttcgttgca
     5221 catcttttct gtgagccaag cagcctgtca aaatagcttt tttctcgggg gatttctttt
     5281 tgatattatt gatctgtcca taaactctgt cctcggccga ttggcgcact ccgcaggtga
     5341 cgaatatgac aaagtcggcc gcatcaatcg aagaggccgg tttaattttg tatttttcca
     5401 aaaacgtagc gattctttcg gcgtcggact tctctccc
//
LOCUS       DATKGS010000098         2659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_212837, whole genome shotgun sequence.
ACCESSION   DATKGS010000098 DATKGS010000000
VERSION     DATKGS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212837"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="serS"
                     /locus_tag="VMQ48_02395"
     CDS             <1..1142
                     /gene="serS"
                     /locus_tag="VMQ48_02395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HUD08919.1"
                     /translation="ELRLEKNKLNDEMQKASDAEKSGLISKGKKIKEDSDKAEAAWKI
                     KNGEFLKELLRIPNMSHPDSPIGKDDSENKEIERYGELPKFDFEPKGHEELMKNLDLI
                     DFERGAKVAGAKFYFLKNEAVLLEQALINYALDTCIQEKFTPFTTPDLAKDSILLGVG
                     FNPRGQESQIYSVENTDLNLVGTAEITMGGYHMDEMIDEENLPLKYVAFSHCFRTEAG
                     SYGRHSAGLYRVHQFSKVEMFAHTLPEKSEEMHTYFKDLEVKIFKGLGVPFRVVDICT
                     GDLGGPAYRKYDLEAWMTSRNDWGEVTSTSDTTDYQARRLNIKVKRAYGKKEFLHMLN
                     GTAIATSRALIAIIENYQQADGSVVIPEILRRWMPGEQKVIKKNG"
     gene            1135..2301
                     /locus_tag="VMQ48_02400"
     CDS             1135..2301
                     /locus_tag="VMQ48_02400"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUD08920.1"
                     /translation="MDNKINLSPIKDMELRASRIPGVVSLAQGVPSFDTPDAIKRAAI
                     EAIEKGKVAKYSLSPGLLELREVITQKLELENKFYDFEEEVIVTAGSIEAITATMLAI
                     LSPGDEVLIPDPTYTSYQPAIKVAGGVPVFVPLDEKNHWAFDIAELEKRITPKTKALL
                     FCNPNNPTGTIFTRNQLHAVAQLAIKHDFYILSDEVYKDFIFDAGEKFYSLAEMRGIR
                     DRLIYIFSFSKAYAMTGWRIAYLATHHELAKKILGVHDALVTCAPVVSQYAALAALEM
                     ADSDLAKYHAIYKNRRDLICRRLDKLKNIFEYQKPDSAYFVFPRIKEDILQKIVPNGG
                     SKEFAIELLEKAKVATVPGSAFGPVGENHIRMCFGRSEEDINLAFDRMDKFFIT"
     gene            2356..>2659
                     /locus_tag="VMQ48_02405"
     CDS             2356..>2659
                     /locus_tag="VMQ48_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08921.1"
                     /translation="MKNIFKSFIQYYLKLLTKFVLWRHKPFIIAVAGTTNKTTVKEYI
                     LKFLREKYGEQEVRGNPRSYNTEIGLPLAILYLESGESSILKWLKILIQAKIEAIPS"
BASE COUNT          786 a          558 c          671 g          644 t
ORIGIN      
        1 aggagcttag attggagaaa aacaagctta atgatgaaat gcagaaagct agcgatgctg
       61 aaaaatcagg attaatctcg aaaggtaaaa agataaaaga agactcagat aaggcggaag
      121 ccgcctggaa gataaaaaac ggtgagtttc taaaagagtt acttcgcatt ccgaatatgt
      181 cgcatcctga ttcgccgatc gggaaagacg acagtgagaa caaagagatt gaaagatatg
      241 gagagctgcc gaaattcgat ttcgaaccga aggggcacga ggagttgatg aaaaatcttg
      301 atttgatcga ttttgaacgc ggggctaagg tggctggagc gaaattctat tttctcaaaa
      361 acgaagcggt gcttctcgag caagcgttga taaactatgc tcttgatact tgtattcagg
      421 aaaaatttac gcctttcaca actcctgatc ttgccaaaga cagcatactt cttggagtcg
      481 gattcaatcc gcgcgggcag gaatcgcaaa tatacagcgt cgaaaatacc gatctgaatt
      541 tggtcgggac agccgagata acaatgggcg ggtatcatat ggacgaaatg atcgacgaag
      601 aaaatttgcc cttgaaatat gttgctttct cccattgttt tcgaaccgaa gcgggaagct
      661 acgggcggca cagcgcagga ctctatcgcg tgcatcaatt ctcgaaagtc gaaatgtttg
      721 cccatacttt accagaaaaa tcagaagaaa tgcatactta tttcaaagat cttgaagtga
      781 aaatattcaa aggacttggc gtgccatttc gggtggttga tatctgcacg ggcgatcttg
      841 gcgggccggc ctatcgcaaa tatgaccttg aagcttggat gacgagccgc aacgattggg
      901 gagaagtgac ttcgacatcc gacacgacgg actatcaggc gcgccggctc aacatcaaag
      961 tgaaacgcgc atatggaaaa aaagaatttt tgcatatgct caatggcacg gcgatagcaa
     1021 catcccgtgc gctaattgcg ataatagaaa actatcagca agccgacggg tcggtcgtca
     1081 ttccggaaat tttgcgcagg tggatgccgg gagaacagaa agttatcaag aagaatggat
     1141 aataaaatca atctttcacc aataaaagac atggaactcc gggcgtcgcg cattcccgga
     1201 gttgtttcgc tcgcccaagg cgtgccgtct tttgatacgc cggatgcaat caagcgggcg
     1261 gcgatcgagg cgattgaaaa ggggaaagtg gccaaatatt cgctctcgcc gggtcttttg
     1321 gaattgcgcg aagtgatcac ccaaaaattg gaattggaaa acaaattcta tgattttgaa
     1381 gaagaagtta tcgtgacggc cggatcgatt gaagccatca cggcgacgat gctggcgatt
     1441 ctttctccgg gggacgaggt tttgattccc gatccaacgt atacgtcata tcagccggcc
     1501 atcaaggtgg cgggaggcgt gccggttttc gtgccgctgg atgaaaaaaa tcattgggcg
     1561 tttgatattg cggagcttga aaagaggatc accccaaaaa caaaagctct tcttttttgc
     1621 aatccgaaca atccgacggg gacgattttc acgcgcaatc agctccacgc ggtggcgcaa
     1681 ctggcgatca agcacgattt ttatatcttg agcgacgaag tatacaaaga tttcatattc
     1741 gatgccggtg aaaagtttta ttccctcgcg gaaatgcgcg gtattcgcga tcgtttgatc
     1801 tatatcttct ctttttcaaa ggcctacgca atgaccggct ggcggattgc gtatctcgcc
     1861 acgcaccacg agctggcgaa gaaaattctg ggtgtccatg acgcgcttgt cacctgcgcg
     1921 ccggtggtgt cgcaatacgc ggctcttgcg gcgcttgaaa tggcggattc ggatctggcg
     1981 aaatatcacg cgatatacaa aaacaggcgg gatttgatct gccggcggct tgataaattg
     2041 aaaaatatat ttgaatatca aaaaccggat agtgcctact tcgtttttcc gcgtatcaaa
     2101 gaagacattt tgcaaaaaat tgtccccaat ggcggatcaa aggaatttgc gatagaattg
     2161 ctggagaagg ccaaagtggc tacggttccc ggatcagcct tcggtccggt cggggagaat
     2221 catattcgga tgtgcttcgg gcggtcagaa gaagatataa atttggcgtt tgataggatg
     2281 gataaatttt ttataacatg attttcctaa cattcgatgt taggagcgcc taacatcgaa
     2341 tgttaggaaa ataccatgaa gaatattttc aaatcattta tccaatatta tctcaaactt
     2401 ctcaccaagt tcgttttgtg gcggcacaag ccgttcatca ttgccgtcgc gggaacaaca
     2461 aacaaaacaa cagtcaaaga atatattttg aagttcttgc gcgagaaata tggtgagcaa
     2521 gaggtgcggg gaaatccccg cagttacaac acggaaatcg gcttgcccct ggcaattctc
     2581 tatctcgaat cgggcgaatc ctcgattcta aaatggctaa aaatattgat tcaagccaaa
     2641 atagaagcta taccctccc
//
LOCUS       DATKGS010000099         3494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_154786, whole genome shotgun sequence.
ACCESSION   DATKGS010000099 DATKGS010000000
VERSION     DATKGS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154786"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VMQ48_02410"
     CDS             complement(<1..762)
                     /locus_tag="VMQ48_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUD08922.1"
                     /translation="MQKYNPKKIEKKWQKIWVKNPKLSAADEKSKKPKFYCLDMFPYP
                     SGDGLHVGHVEGYTASDIYSRYQRMIGKNVLHPMGWDAFGLPAENFAIKTGVHPAKST
                     QKNIKTFTKQINSLGLSYDWSREIDTSSPEYYKWTQWFFLFLYKNGLAYRKKAKVNWC
                     ESCKTVLANEQVEDGVCERCKKEVVQKDLEQWFFRITDFAEDLIKDLDKIDWPESTKI
                     AQKNWIGKSEGAIVKFSISNFQFPNKSQFKNDQRYL"
     gene            complement(867..1340)
                     /locus_tag="VMQ48_02415"
     CDS             complement(867..1340)
                     /locus_tag="VMQ48_02415"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HUD08923.1"
                     /translation="MLHTCEAALVTCEDYRLHQRKDGRNYVAKYILKLGVDCDLITRA
                     GGVQDLLRPGGTGFADPIIRDVDVSHRLHSAEILVFLNHEDCGAYKGFKFRDRDEELR
                     RHYQDLRRTLRMMHLLFPKKRLIGSFAELKPGTTDVFDIKKMFEFKPGAKKIVAV"
     gene            complement(1463..1774)
                     /locus_tag="VMQ48_02420"
     CDS             complement(1463..1774)
                     /locus_tag="VMQ48_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08924.1"
                     /translation="MVGGNKSFSPLGFIVLLESSKDFAGRNWAERYVTKIAGRPKLEV
                     VSVFESLKVAKKIQQLLISCRRVTLISRLYSVGHKTEAIIQGMLDFYLVKKEIVHMII
                     I"
     gene            complement(1796..2239)
                     /locus_tag="VMQ48_02425"
     CDS             complement(1796..2239)
                     /locus_tag="VMQ48_02425"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HUD08925.1"
                     /translation="MKTEFKYKDIHSCEAVVLACIDFRFWKETMKFVEDELGIASYDF
                     PKMPGAAKAINDCQNEIDVPMKCIGVPCELHHVEKIVIVNHADCGAYGGAKEFSGDDE
                     AEQKFHEVQLKSAKEKILKYYPGKEIILAYAKLVDEGENIEFIIL"
     gene            complement(2366..2806)
                     /locus_tag="VMQ48_02430"
     CDS             complement(2366..2806)
                     /locus_tag="VMQ48_02430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HUD08926.1"
                     /translation="MDKNKIKFKIVTPEKTVFEDSIDQVTLPTQEGEITVLPEHIPLI
                     AVLAPGELTVKQDGKEIAMAVSGGMIEVRKNELTILADTAERAEEIDIKRAEEARERA
                     EKLKDEKIRMDETEYAAVAALMERNLARIKVAKKHLTRRGMRIE"
     gene            complement(2913..>3494)
                     /locus_tag="VMQ48_02435"
     CDS             complement(2913..>3494)
                     /locus_tag="VMQ48_02435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HUD08927.1"
                     /translation="IPSAVGYQPTLAEEMGRLQERITSTKKGSVTSVQAVYVPADDLT
                     DPAPATTFGHLDSTVVLSRNLTELGIYPAVDPLDSSSTILDPKIVGEEHYQTARGVQK
                     ILQKYKDLQDIIAILGVEELSDEDKLAVSRARKIQKFLSQPFFVAEQFTGSPGKYVKL
                     SDTIRSFKEILDGKHDDKNEQMFYMKGSIEEVK"
BASE COUNT          864 a          862 c          669 g         1099 t
ORIGIN      
        1 taaatatctt tgatcatttt taaattggga tttgtttgga aattgaaaat tggaaattga
       61 aaattttaca atagctccct cgcttttacc gatccaattc ttctgcgcga ttttggttga
      121 ttccggccag tcgattttgt ccaagtcctt gatcagatct tccgcaaaat cagtgatgcg
      181 gaaaaaccac tgctcgagat ctttttgcac gacttctttt ttacaacgct cacagacgcc
      241 gtcctcaact tgctcgttcg ccagaaccgt cttgcatgat tcacaccaat tcactttcgc
      301 ttttttgcga taggccaatc catttttata caaaaacaaa aagaaccact gtgtccattt
      361 gtaatattcg ggacttgaag tatcgatttc ccgcgaccag tcataggaaa gtccgagtga
      421 attgatctgc ttcgtgaaag ttttgatatt tttttgcgtc gacttggcag gatggacacc
      481 cgttttgata gcaaaatttt ccgccggaag tccgaatgcg tcccagccca tcggatgcaa
      541 aacatttttc ccgatcatcc gctgatagcg ggaatatata tcgctcgccg tataaccttc
      601 cacgtgtccg acgtgcaacc cgtcaccgga cggataaggg aacatgtcga gacaatagaa
      661 tttcggcttt tttgatttct catccgcggc cgaaagtttc gggtttttga cccatatttt
      721 ttgccacttt ttctcaattt ttttcggatt atatttctgc ataaaatatc aaaattacac
      781 tataacttta tcccattttc cggtttttat caagaaaaaa agcccggggt aatgcttccc
      841 gggcttcgaa tttgcaaggc cttccatcag accgcgacga tcttcttggc gcctggtttg
      901 aattcgaaca ttttctttat gtcaaaaacg tccgttgtcc caggcttcaa ttcagcaaaa
      961 gaaccaatga gcctcttttt tggaaacaga agatgcatca tccttagggt tcgacgaagg
     1021 tcttggtaat gtcttcgtag ctcttcgtcc ctatccctga acttaaagcc cttataagca
     1081 ccgcaatctt catgattgag aaatacaagt atttctgcgc tatgcaaccg atgagaaaca
     1141 tcaacgtctc taatgattgg atcggcaaac ccggtccctc caggtctcaa taagtcctgt
     1201 actccaccgg ctcttgtaat aagatcgcag tcgacaccaa gcttaaggat atacttggcg
     1261 acataattcc tgccgtcctt cctctggtga agcctataat cctcgcaagt aaccaatgct
     1321 gcttcgcatg tatgaagcat atgcccccca tttgtcagaa taaaaacagc tctaaagaac
     1381 cgctagcatt atagcagaaa taatagattt gtcaagaaaa aacagctcgc cttttggcaa
     1441 accgggtctt taatcgatct tttcagataa ttatcatgtg gacaatctct tttttcacga
     1501 gatagaaatc caacatcccc tggattatag cttccgtttt atggcctacg gaatataatc
     1561 ttgaaatgag agtcacccgc cggcaactga tcaaaagttg ctgaattttt tttgcaacct
     1621 tcaaggattc aaaaactgat accacctcca atttgggtct tcccgctatt tttgttacgt
     1681 atctctccgc ccaatttctt cctgcaaagt ccttggaaga ttccaaaaga acaatgaacc
     1741 ccagtggcga aaaacttttg tttcctccaa ccatttcgtc tctccttttt ggcagctaaa
     1801 gaataatgaa ctcaatattc tctccttcat caaccagctt tgcataggcc aaaataattt
     1861 cttttcccgg gtaatatttc aaaatttttt ctttggcgct tttgagctgg acttcgtgaa
     1921 atttttgctc cgcctcgtcg tcgccagaga attctttcgc tccgccatag gcgccgcagt
     1981 cggcatggtt cacgatgaca atcttttcca cgtggtgcag ttcgcacggc acaccgatgc
     2041 acttcatcgg aacatcgatt tcgttctggc aatcattgat agctttggcc gctcccggca
     2101 ttttgggaaa atcatatgag gcgattccca attcatcttc cacaaatttc attgtctctt
     2161 tccaaaaacg aaaatcgata caagcaagga cgacggcttc gcaggagtgg atatctttgt
     2221 acttaaattc tgttttcata aatcttgtct tgttatttgc aaaattttta aattttgtaa
     2281 ttttcaattt tcaaacaatt aatcaatttc taaatttcaa aattttgaaa ttgtttttga
     2341 aattgaaaat tccggaattc taaaattatt ctattctcat tcctcttctc gtcagatgtt
     2401 tcttcgccac tttgatccgc gccaggttcc gttccatgag agcggctacg gcggcgtatt
     2461 ccgtctcgtc catccggatc ttttcgtcct tcaatttctc ggcccgctcg cgcgcttcct
     2521 ctgcccgctt gatatcgatt tcctccgccc gttcggccgt gtcggccaaa attgtcagtt
     2581 cgtttttgcg cacttcgatc atcccgcccg aaacggccat cgcgatttct ttcccgtctt
     2641 gtttgaccgt cagttcgccc ggcgccaaaa ccgcaatgag cggaatgtgc tccggaagca
     2701 cggtaatttc gccctcctgg gtaggaaggg tgacctggtc gatactatcc tcaaagaccg
     2761 tcttctctgg ggtcactatc ttaaatttta ttttattctt gtccaatgct ttttctcaat
     2821 ttttaaattt tataattttc aattttcaaa caattcttta attttaaaat ttctaaattg
     2881 tttttgaaat tgaaaattcc ggaattctga aattatttca cttcctcaat acttcccttc
     2941 atatagaaca tctgctcgtt cttgtcatcg tgtttgccgt ccaaaatttc cttgaaactg
     3001 cggatcgtgt cggaaagttt cacatatttt cccgggctgc cggtgaattg ttccgcgacg
     3061 aaaaacggct gggacaaaaa tttctggatc ttccgcgctc ggctcacggc caatttgtct
     3121 tcgtccgaca actcttccac cccgagaatc gcgatgatat cctgaaggtc tttgtatttc
     3181 tgcaaaattt tctgtacgcc tcgggctgtt tgatagtgct cttcgcccac gattttcgga
     3241 tcaaggatgg tcgaagatga atcgagcgga tcgacagccg gatatattcc aagctcggtg
     3301 agatttcgcg acagaacgac cgttgaatcg aggtgcccga aagtcgtcgc cggcgccggg
     3361 tcggtgagat cgtccgccgg aacatacacg gcctgcacgg acgtcacgga accttttttg
     3421 gtggaagtga ttcgttcctg aagccgtccc atttcttccg cgagcgtcgg ctgatatccc
     3481 accgctgacg gaat
//
LOCUS       DATKGS010000100         6602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_53242, whole genome shotgun sequence.
ACCESSION   DATKGS010000100 DATKGS010000000
VERSION     DATKGS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53242"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /gene="grpE"
                     /locus_tag="VMQ48_02440"
     CDS             <1..189
                     /gene="grpE"
                     /locus_tag="VMQ48_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HUD08928.1"
                     /translation="QLEDVLKNNDIEEILVKPGDKFNPALHEAIGGDGKKQKVARVVQ
                     KGYRLNGRILRAARVEVA"
     gene            326..2260
                     /gene="dnaK"
                     /locus_tag="VMQ48_02445"
     CDS             326..2260
                     /gene="dnaK"
                     /locus_tag="VMQ48_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HUD08929.1"
                     /translation="MSKILGIDLGTTNSAMAVVEAGSPKIVENKEGARTTPSMVAISK
                     SGERLVGLLAKRQAVTNPENTLFSIKRLIGRRFEDQEVQRDVKLMPYSIVKANGGVKI
                     KMGDKEYTPQEISAMILGKLKADAEAKLGETITEAVITVPAYFDDSQRKATIEAGEIA
                     GLTVKRIINEPTAAALAYGFDKKKDEKIAVYDLGGGTFDISILEVGNDTVEVKSTNGN
                     THLGGDDFDQTIIKWILDEFKKDQGIDLSKDSLALQRIKEAAEKAKIELSTAQETEIN
                     QPFITSDASGPKHMVMKLTRAKMEELVGELVEKTLEPCKKALADAKLSASDINEVVMV
                     GGMTRMPLVLATVEKFFGKKPNATVNPDEVVALGAAIQGGVLTGDVKDVLLLDVTPLT
                     LGIETMGGVRTPLINRNTTVPTSKSQTFSTAADNQPSVEIHVLQGEREMAADNKTLGR
                     FMLDGIPPSPRGVPQVEVSFDIDANGVLSVKAKDKATGKEQSIRIEASTGLSKEEVEK
                     MAKDAETHAEEDKKKKEAVEARNLADTLVYTTEKALRDAGEKISSDKKKPVEEKIEAL
                     KKVKDGEDIEAIKKATEELSQEAQKIGQELYAAAQEADKAKAGEAKPEEKPASDESTA
                     GKKDGEVKEGEVVDEGEKKE"
     gene            2291..3319
                     /locus_tag="VMQ48_02450"
     CDS             2291..3319
                     /locus_tag="VMQ48_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08930.1"
                     /translation="MNKNKIFAYSVLALSVVAAWISWWVLAGAIFGQMRHIWLYPVLA
                     FSVWGIAFTLSAIFVRDRKFLYGSFVAAGVGYLIFFGPSLSVLGVLLAVLIFILTEIQ
                     AKREIARGITINFYQLVSHTLKYFVTAVCVVIAIAYYFSIIEKAATPTPTFIEGKTLQ
                     TEMDWGLKAAGFVLPDDKKSMIDDIADGETVDDFLSKNFVDPGINENMDAANSVVPAN
                     ATDTTKLIGDAAAGKIKEEMLAKSKKDLSKQLGVDVVGEQPMKNVLMAYIDKTERGFF
                     DYSGTDKFYIPVILAFGIFLTARILGTAVDLLLGLLILGIIRLFRKTGVIQVSRVQRE
                     GAVIDYSI"
     gene            3324..4448
                     /gene="dnaJ"
                     /locus_tag="VMQ48_02455"
     CDS             3324..4448
                     /gene="dnaJ"
                     /locus_tag="VMQ48_02455"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUD08931.1"
                     /translation="MSKDYYDTLGVSKEASDDDIKKAYRKLAHKHHPDKPGGDEARFK
                     EVNEAYQVLSDKEKRNQYDQYGQTFEQAQAQGQGGFSGFDGFRDFSSYAEGFDFNFGG
                     GRSNRQGSSGFEDIFGDIFEQAGFGGGGRRSRAARGGDIQVDIEITFAEMAHGTEKEL
                     EIYKKVRCSVCDGGGVEPGAKKVECPTCKGEGQIRSNRRTILGTFQQVSTCPECQGEG
                     KVPEKKCKKCGGDGRVREYEKIKIKIPAGIKDGQTIRLENMGEAGEKGGGSGDLYVNV
                     HVATDKRFERKEDDIHSEAKISFSQAALGDKIEVETINGDVKLKIPAGTQSGEIFRLK
                     GHGLKHLSHFGHGDQYVKIQVVTPKDLSREEKEIFENLKK"
     gene            4530..5039
                     /locus_tag="VMQ48_02460"
     CDS             4530..5039
                     /locus_tag="VMQ48_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08932.1"
                     /translation="MSNGMNLNNLLGNIGIKSNLAGDVSFLIIFLLVSFVVSFALGKH
                     RILVSLLGVYAAYAVVNLASFDFLKDAQTKTLIFLAVIIGFIMMFSRIIRGSVSGHGP
                     VFYAKLTIGTAIVIGLSLSIILVWYPAKDLENVVTPSVKMYFTDGFYRFLWAIAPLVY
                     LGIVRKRID"
     gene            5105..5177
                     /locus_tag="VMQ48_02465"
     tRNA            5105..5177
                     /locus_tag="VMQ48_02465"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5138..5140,aa:Lys,seq:ttt)
     gene            5248..5321
                     /locus_tag="VMQ48_02470"
     tRNA            5248..5321
                     /locus_tag="VMQ48_02470"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5282..5284,aa:Ile,seq:gat)
     gene            5347..5419
                     /locus_tag="VMQ48_02475"
     tRNA            5347..5419
                     /locus_tag="VMQ48_02475"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5380..5382,aa:Ala,seq:tgc)
     gene            5455..5886
                     /locus_tag="VMQ48_02480"
     CDS             5455..5886
                     /locus_tag="VMQ48_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08933.1"
                     /translation="MLALEAQTYVPGEMRRYETIKREPRILRPVGWFLPSEFVACQTI
                     KGKWKIGQFIEVNGDPPCLMICMNLIKGSCQAECESHCEHRYWAMGDQSGVMGKGDLL
                     RIIADPLSLTVFGFPYTVSAYNQVRRLLRLINNAGKRQKSC"
     gene            6358..>6602
                     /locus_tag="VMQ48_02485"
     CDS             6358..>6602
                     /locus_tag="VMQ48_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08934.1"
                     /translation="MARESMGDVGSDFFDDLLSGSAAFGIPNRTADDQIFHAVQYGFS
                     WGHSAALVVRRRIGHADPRRVKIHLRPPGVEFSGFFRR"
BASE COUNT         1906 a         1322 c         1797 g         1577 t
ORIGIN      
        1 cagctcgaag acgttttgaa aaataatgat attgaagaga ttttggttaa gccgggagac
       61 aaattcaatc cggcgcttca cgaggcaatc ggcggagacg gaaaaaagca gaaagtggca
      121 agggtcgttc agaaaggcta caggctgaac gggcgaatat tgcgagcggc gagagtggaa
      181 gtagcgtaaa atttcaaaat ttcaaaattt tcaatttcag aaacaatttc aaaattctga
      241 aatttagaaa ttaaataatt gttttgagaa ttgaaaatta ttgaattctg aaattataat
      301 ttattaatta tttatttata aatttatgtc caaaattcta ggcatagact taggcacaac
      361 gaatagcgcg atggcggtgg tggaagccgg atcgccaaag atcgtggaaa acaaagaagg
      421 ggcgcgaacg acgccttcaa tggttgcgat ctcaaaatcg ggcgaaagac tggttgggct
      481 cttggcgaag cgccaagccg tcaccaatcc ggagaatacc ctgttttcaa tcaaaagatt
      541 gatcgggcgc cgttttgaag accaggaagt gcaaagagac gtgaaactga tgccgtattc
      601 catcgtgaaa gccaatggtg gcgtgaaaat caagatgggg gacaaagaat atactccgca
      661 ggaaatatca gcgatgatcc tgggaaaact caaagccgat gcggaagcga agcttggcga
      721 gacgatcact gaagcggtta tcactgttcc ggcctatttt gacgactcac agagaaaagc
      781 gacgattgaa gcgggtgaga ttgccggact cacggtgaaa cgcatcatca atgaaccgac
      841 ggcggccgcg ctcgcgtatg gttttgacaa aaagaaggat gaaaaaatcg ctgtctatga
      901 tcttggaggc ggaacttttg atatttccat tttggaagtg ggaaatgaca cagtggaggt
      961 gaaatcaacg aacggaaaca cgcatcttgg cggtgacgat tttgaccaaa ccatcatcaa
     1021 atggattctc gacgaattca aaaaagatca agggattgat ctctcaaaag atagtttggc
     1081 tttgcagagg atcaaggaag cggcggaaaa agccaagatc gaactctcga ccgcgcaaga
     1141 aactgaaatc aaccaacctt tcatcacttc ggacgcgagt ggaccaaagc atatggtgat
     1201 gaagctgacg cgggccaaaa tggaagaatt ggtcggagaa ttagttgaaa aaacgctcga
     1261 gccttgcaaa aaagcgcttg cagatgcgaa attatcggcg agtgatataa acgaagtggt
     1321 gatggtcggc gggatgaccc gcatgccgct cgttctcgcg acagttgaaa aatttttcgg
     1381 gaaaaaaccg aacgcgacgg tgaacccgga tgaagtcgtg gcgcttggcg cggcgatcca
     1441 gggaggagtg ctgacaggcg acgtgaaaga cgttctactt cttgatgtga cgccccttac
     1501 tctcggaatc gagactatgg gcggtgtccg gacgccactc atcaaccgca atacgacagt
     1561 tccaacttca aaatcacaga cattttccac agcggccgac aatcagccga gcgttgaaat
     1621 ccatgtactt caaggcgagc gagaaatggc ggccgacaac aagactctgg gaagattcat
     1681 gctggatgga atcccgcctt ctccgcgcgg cgtgccgcaa gtggaagttt cctttgacat
     1741 cgacgccaat ggagtcttga gcgtgaaagc caaagacaaa gcgaccggga aggagcaatc
     1801 aatccggatc gaagcctcca cgggactttc caaggaagaa gtggaaaaaa tggccaaaga
     1861 tgccgaaacg cacgccgaag aggacaagaa gaaaaaagaa gcggtcgaag cgcggaatct
     1921 tgccgacaca ttggtataca cgacggaaaa agctttgcgt gatgccggag aaaaaatctc
     1981 gtccgacaag aaaaaaccgg ttgaagaaaa aatagaagcc ctgaaaaagg tgaaggacgg
     2041 cgaggatatc gaagcaatta aaaaagcgac cgaagaactt tcgcaggaag cccaaaagat
     2101 cgggcaggag ctctatgcgg ccgcgcagga agccgacaaa gccaaggccg gcgaagcaaa
     2161 gccggaagag aaacccgcct ccgatgaatc tacggcgggc aagaaagacg gcgaagtaaa
     2221 agaaggcgaa gtagtcgacg aaggcgaaaa aaaagagtaa aataaataca gaaatttata
     2281 cactatctcc gtgaacaaaa ataaaatttt tgcttattcc gttctggctt tgagcgtggt
     2341 tgcggcttgg atcagctggt gggttttggc cggcgctatt tttggacaaa tgcgccacat
     2401 ctggctctat cccgtcttgg ctttcagtgt ttggggaatc gctttcactt tgagcgcgat
     2461 attcgtcaga gatcgaaagt ttctatatgg aagttttgtc gctgcgggtg tcggctatct
     2521 catttttttt gggccgagtc tgtccgtact tggagtcctg ttggcagtct tgatattcat
     2581 cctcactgaa atccaggcaa agcgagaaat tgcgcgcggc ataacgataa atttctatca
     2641 gcttgtcagc catacgctta aatattttgt gacagccgtc tgcgttgtga tcgctattgc
     2701 ctactatttt tcgatcatag aaaaggcggc gacaccaacg ccgacgttca ttgaaggcaa
     2761 aactcttcaa acggaaatgg attggggtct caaagcggcc ggtttcgttc ttccagatga
     2821 caagaaaagt atgatcgatg acattgcgga tggagaaacg gtcgacgact ttctctcaaa
     2881 aaatttcgtc gatcccggaa taaacgaaaa tatggatgca gcgaacagcg tcgttcccgc
     2941 gaatgcaaca gacactacca agctcattgg cgatgcggcg gccggcaaga taaaggaaga
     3001 aatgttggcg aaatcgaaaa aggatctttc gaagcagctg ggggtagacg tagtgggaga
     3061 acagccgatg aaaaacgtgc tgatggcgta tatcgacaag acagaaagag gattttttga
     3121 ttattcggga acggacaagt tctatatccc ggtcattctc gcatttggaa tattcttgac
     3181 ggcaagaatt cttggaacgg cggtggattt gcttctgggg ctgcttattt taggaataat
     3241 aaggttgttt cggaagaccg gcgttatcca ggttagtcgg gtgcagaggg aaggggcagt
     3301 gattgattat agcatttagc attatgtcca aagactatta cgatactctc ggcgtttcga
     3361 aagaggcgtc cgatgatgat ataaaaaaag cctatcgaaa attggctcac aaacaccacc
     3421 cggacaaacc cggtggcgat gaagcgcgct ttaaggaagt gaacgaggcc tatcaagtcc
     3481 tttccgacaa ggaaaaacgc aaccaatacg atcaatacgg ccagacattc gagcaggctc
     3541 aagctcaagg gcaaggcggg ttcagtggat tcgacggatt tcgcgatttt tcttcgtatg
     3601 cggaagggtt tgatttcaat tttggcggag ggcgaagcaa tcgccaggga tcatccggtt
     3661 tcgaagatat tttcggagat atttttgaac aagccggttt tggtggaggc ggaagaaggt
     3721 cgcgtgcagc gagaggcggg gatatccaag ttgatattga aatcactttt gctgaaatgg
     3781 cgcacgggac ggaaaaagaa ttggaaatat acaaaaaagt gagatgttcg gtttgcgatg
     3841 gaggtggggt cgaaccggga gcgaaaaaag ttgagtgccc gacgtgcaaa ggggagggac
     3901 aaattcgcag caaccggcga acgattctgg gaacattcca gcaagtgagt acgtgtcccg
     3961 aatgtcaggg cgaaggaaaa gttccggaga aaaaatgcaa aaaatgtggc ggagacggac
     4021 gggtgcgcga atatgaaaaa ataaagatca aaattccggc tgggatcaag gatggccaga
     4081 cgatccggct cgaaaatatg ggagaagccg gggaaaaggg cggcgggtcg ggagatctct
     4141 atgtaaatgt ccatgtcgca accgataaaa ggtttgaaag aaaagaggat gatattcact
     4201 cggaagccaa aatatctttt tcgcaggcgg cgctcgggga caaaattgaa gtggaaacga
     4261 tcaatggcga tgtgaagctc aaaattcccg ctggcacgca gtcgggggaa atttttcgtc
     4321 tcaaggggca tggtctcaaa catttgtccc attttggcca cggagatcaa tatgttaaga
     4381 tacaagtggt gacgccgaaa gatttgagcc gggaagaaaa agaaattttc gaaaatctaa
     4441 aaaaataaaa atacaaatat gaatcccgtt agacatcact tagaattatt taatctggtg
     4501 atatgacggg ctaaaacaaa taattattaa tgtctaacgg gatgaacctc aacaatctcc
     4561 ttggaaacat cggaatcaaa agcaatttgg ccggcgacgt gtcttttttg atcatatttt
     4621 tgcttgtgag ttttgtcgtt tcgtttgcgc ttgggaaaca ccggattttg gtttcgcttt
     4681 tgggagtcta tgcggcttat gcagtcgtga atctggctag ttttgatttt ctgaaagatg
     4741 cacagacgaa aacgttgata ttcttggctg ttatcattgg cttcatcatg atgttttcgc
     4801 ggatcatccg cggaagtgta tcaggacacg gtccggtttt ctatgcaaaa ttgacaatcg
     4861 ggacggcgat cgtgatcggt ctttcgcttt cgattatctt agtctggtat ccggccaaag
     4921 atctggaaaa tgtggtgacg ccaagcgtaa aaatgtattt cacggacggt ttttaccgat
     4981 ttctctgggc tatcgccccg ctggtatatt tggggattgt gaggaaaagg attgactgat
     5041 tttgtttttg tagtataatt ctatcgttgc ctaaaattag gccgttttag gtattgtttg
     5101 tttcgggtcg ttagctcagt cggtagagca gtggcctttt aagccaacgg tcgcaggttc
     5161 gatccctgca cgacccacac ccaaaaaata tttttggaat ttccaatttc caaatcccaa
     5221 tttccaattg tggaagattt tttataaggg cgattagctc agttggttag agcgcgtcac
     5281 tgataatgac gaggtccgag gttcgagtcc tcgatcgccc acaactttgg aggacgtagt
     5341 gccgacgggc ccgtagctca tctggtagag cgcctgtttt gcatacagga ggtgaggagt
     5401 tcgagtctcc tcgggtccac gagtaatata aaattagttc aaaaaaagag gaggatgcta
     5461 gccttggaag cacaaaccta tgtgccaggt gaaatgaggc ggtatgagac tatcaaacgt
     5521 gagcctcgca tcttgaggcc ggtaggatgg tttttaccaa gtgagtttgt ggcttgccaa
     5581 acgatcaagg ggaaatggaa aatagggcag tttattgaag tgaatggtga tccgccctgc
     5641 ctgatgattt gcatgaactt gatcaaggga tcctgtcaag ccgagtgtga atcccattgc
     5701 gagcatcgat attgggcaat gggagatcag tctggggtta tgggaaaagg tgatctgttg
     5761 cgtattatcg ccgatccttt gtcgctgaca gtctttggct ttccctatac agtaagcgcc
     5821 tataatcaag tcaggcgttt gctccgtttg atcaacaacg cggggaaacg gcaaaaaagt
     5881 tgctgaaaaa accctcacga aaaagaagaa aggttcggag ccaattgtgg cgccgaacct
     5941 ttttcaaatt gcgagatgag atcatctgaa ggccgctctg cggagtggcc agtggggagc
     6001 gagtgatttt tctccccgtc taaaaaatac gggagcactc actgcaggtg gttttcaagg
     6061 gagatttttg ctctagactt tgggagtcgc actccatggc aggcacgatc tttgtgctgc
     6121 cgcattccgg acactgagcc tgtttttttt cttgattcac gtttcctcca gaaaattttg
     6181 aaataatttg ctgacattat cagcaaggag ccctccaaga aatattatag cagattaaaa
     6241 aaaatttttc aaaaagaaaa cccctcaggt gtgcggaccg gaggggcagg gtgttctttg
     6301 gaacgataaa tttttcagca atttcctcct gctgcgcgga tttggttatg gtgtcatttg
     6361 gcccgcgaga gcatgggcga tgtcggatcg gatttcttcg atgacctgct ttcgggttct
     6421 gccgccttcg ggattcctaa taggacggcc gatgaccaga tatttcacgc cgttcagtac
     6481 ggcttctcct ggggtcatag tgcggccctg gtcgtccggc ggagaatagg gcatgcggat
     6541 ccccggcgag taaagatcca tttgaggcca ccgggtgtgg agttcagcgg attctttcgg
     6601 cg
//
LOCUS       DATKGS010000101        12620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_97879, whole genome shotgun sequence.
ACCESSION   DATKGS010000101 DATKGS010000000
VERSION     DATKGS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12620
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97879"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VMQ48_02490"
     CDS             complement(<1..607)
                     /locus_tag="VMQ48_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08935.1"
                     /translation="MPARLVAKIKNKKWKIILFLSIFAVGIFLRSYHFRNWLDFESDQ
                     ARDLSITEKVVDKGGSWPLLGPDMSHSQDGKFRFHVGPIYYYFQIISGHLFGVHPETA
                     AYPDLFFAILSIPLFYFFLKRYFSGNWSLALTGLYAVSFYIIQYSRFAYNTNPIPFFV
                     ILFLLSLLEFLEKREKVSWTWAILAGIALGIGVQLHVTLLLI"
     gene            complement(655..2268)
                     /locus_tag="VMQ48_02495"
     CDS             complement(655..2268)
                     /locus_tag="VMQ48_02495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08936.1"
                     /translation="MSRDQESFWKKNKKKITVIIAIAIFSIGLFLRATHFHDWLDFGD
                     DQVNDASIVTKVVDHNAPWPLLGPKMSHTGNGGRKGQYHLGSMFYYFEITSAKLFGNR
                     PDALAYPDLLFDILTLPLFYIFFRRFFSTNITLALTGLYAVSFYVLNFSHSALNPNLI
                     PFFVLLFLFSLLEFLAHKEKTSWKWAVFLGLALGVGIQLHAILLVLLPLVTAVVFFFL
                     LRKYRQDWKKWGAVLLIALILNAGQIVSEIRTNFANTRIFFISLQSSGSGSGNGTLLN
                     NIPLDISCHVQANAYILSSLGDSQCNFSYTNPAQWRKISLGSWANLLFGILFSVFGYG
                     FLIYKFRREKEEKRKYLLGLALLFVSCFFVVFLPVVASQYRYFLPVFFAPFFFLGFIL
                     DYFARKNFQKFLPFLVLIYSLVLAANYLSIQTAAKELLTRARNGKDHIVLGETETMVN
                     YIIAHTPGGQREAYLMTSMFSHNCVKPLKFIAGENRFKLLIASDVDHIPPGKQAFYIQ
                     SSEGGSDETSSKFAASENFGQETIYQLRN"
     gene            2439..3113
                     /locus_tag="VMQ48_02500"
     CDS             2439..3113
                     /locus_tag="VMQ48_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUD08937.1"
                     /translation="MKILVVEDNEKLAKSLKKGLEQEGHAADYLTDGEAGQRRLEVSA
                     EDYDLVILDLMLPKRDGIAVCKNWRDQNIMTPVIMLTAKDADDDKIFGLDSGADDYLI
                     KPFSFPELLARIRALLRRPKTTLPSELKIKDLVLNVSTRKVFRGQREVHLTLKEFAIL
                     EYLVRQPNQVLNREQIISHVWDFAFDSFSNLVDVHIKNLRKKLEKKNDEKFLETIRGI
                     GYRIKG"
     gene            3058..4086
                     /locus_tag="VMQ48_02505"
     CDS             3058..4086
                     /locus_tag="VMQ48_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD08938.1"
                     /translation="MMKNFWKQFEALVTGSKADLFSSARLRLTAYYLLIMTGVLVIFS
                     AVLYFSFAYVLKDNIDTQFPDVPDQGDLAIVQTMSDAKDGVVVIDLTAFALTIVLGYS
                     LAGRTLRPIEDVLEQQKEFSANASHELRTPLAVMKSESEIALNNPKTSTADLRAVIES
                     NLEEINRMSQMTENLLKLSRVETKKEKIYFSEIELGEFVREAANRISSRTSQKGVSIE
                     VEKTDSGKILGNRDDLVSVASNLIQNGVDYNKPGGAVRVSVENSRQEMRLVVEDNGLG
                     ISEDDLPHIFERFYKADKSHSRNFSNAGIGLSIVKRIIDKHNGSIKIESILGKGTKIV
                     VSFPSAGA"
     gene            complement(4088..5269)
                     /locus_tag="VMQ48_02510"
     CDS             complement(4088..5269)
                     /locus_tag="VMQ48_02510"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HUD08939.1"
                     /translation="MRRSLHSSKAPIGWYGKRNSLGKRILRRIKFAAIVLVFLLVGLV
                     LFLKLNTKGAAVLTDNVLRPLLGDNRVVYLEKIFFNSSDLVQRLTHNSGSNIAPHFED
                     QGSGENLAGGSLNLTPLKVDDSLKPIDGEGVWRNRPLGLFPNQEVMAYTFLRTDPARS
                     YSITTIVQMDTNALRLASVAGTKQPGGPVGKPGPGVVPKDIIGSGKLVAAFDGGFQYK
                     DGAFGMIVGDKTYLPLKNDLGTLVGYNDGSLKIFDYTGQSLGDNVAFVRQNCPMLVTN
                     GQISVTDPRSKALWGRLAAGTVDIYTWRSGIGLTKEGNLLFAVGNNLTPDTLASALKS
                     AGAVNAIQLDINPIWVRFNIFDPTGSGKYTSTTLIKDLQDGSKQYLGGYEKDFFYVYA
                     R"
     gene            5414..5692
                     /locus_tag="VMQ48_02515"
     CDS             5414..5692
                     /locus_tag="VMQ48_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08940.1"
                     /translation="MKYMKKENIIIAVIVVVIVVVGAFAFFNSKKAKKTAKAKTQSSQ
                     TQTQTKTKSKKNAPANGTNQNSASGTDNTSNTPNTSNADTSNSNPTSN"
     gene            5742..6797
                     /locus_tag="VMQ48_02520"
     CDS             5742..6797
                     /locus_tag="VMQ48_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUD08941.1"
                     /translation="MNRKKIAILILIVLAIIVLSLFFFRSPQKTKKTNLLPANPPTSL
                     DLHSFKGVITLITDVVTGNIPEVAKNSTNQAAAPSTISKTQGGFSFAIIGDTKLFDDS
                     PASGNLQKAVGSISKQNVDMVFAVGDLVFKCGDTSSCENSYGDWKTAMSPLMSKTYEV
                     VGNHDRSGGNLADDVWQKEFDLPINGPADFQKQTYSFDFQNSHFVVLDTEKPRGGVVN
                     LEQRIWLEQDLAAHKLQHTFVLFHEPAFPTSQFKRKSLDVDTRQRDALWKIMVQNNVT
                     AVINGHEHFFTRKKVGSIYQFIVGNTDAPENAIPIPKLSDYSYVGNIYAIVTISGAKM
                     NMSLYSVDGKLINSFDF"
     gene            complement(6862..7248)
                     /locus_tag="VMQ48_02525"
     CDS             complement(6862..7248)
                     /locus_tag="VMQ48_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08942.1"
                     /translation="MARRKKLWRKLKVPKPITCNTGEQAPAYQAIEDFFRMVRERIEK
                     TYPNWPLEFKIELYPLYPERLVGKTGGGPTAELKVLFCGDRPVCSVLVTSTDLNFDHF
                     SFALYEPRFSWIRMYWEYQREYKEEN"
     gene            7396..9117
                     /locus_tag="VMQ48_02530"
     CDS             7396..9117
                     /locus_tag="VMQ48_02530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08943.1"
                     /translation="MAPKTKTTKTKSKPAIKTAIKSKSPLKKTAVEKKPAVEKSQKDI
                     SFKLYILLAVLIVVGALFLRTYKLGTLPPGVYPDESVNGIDAITANAAHHWQLFYENN
                     NGRESLFMNLIALGFSLFGISVVTLKMWSVFFGTLTVLGMIVLGEELFASRRAGLVAG
                     FLTATSFWAIDFSRISFRAIMLPFVLVFTVFFLIRGIQKKKIIDYVLAGLFFGLGLHT
                     YIAFRIAPLILVVLLVAFIANRKRFLATHWKEISAFLVATILVALPMLIDFVHNPEHF
                     SGRTGQVSLFTPEVNQGHLLPMLGVSIAKSLAKYSFFGDQNWRHNLPPWPELFPTIGI
                     FFLAGFLYFIYEFFYLAWRRIRAGERSERLILVALLLSWFFAMLIPEAITNEVPHALR
                     SIGTMPVAILMAVFAIEGAWKWADNKEKLKKYKEAIWTLMIVLIVGSGIWSVRMYFVD
                     WGGNPNVPGAFDEGYLNMSNYINSLPASTPKYIVDNGPGIKMQDGLQTSAEVIKLFTY
                     QKADNVMFVEPDVDLSTLKTPSKIFLMNADDGFVSYLRQSFPASRVEKIDVREGMDTG
                     FTMIDVK"
     gene            9154..11664
                     /locus_tag="VMQ48_02535"
     CDS             9154..11664
                     /locus_tag="VMQ48_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HUD08944.1"
                     /translation="MPRLEKKIEQPSKKPEFELKDLATAEEYLDWKKNVPVGVRQIVE
                     TVIRGNKLQPSQTGLALSDQFLSALGESLASDEQVDLSGADKRVFNLLDPLRRIETKG
                     GHLNVFETLRNPEASWNLKRKIFEMQIKPALDWLAERDLEKIAEEERRALESEQTPQE
                     KQPDRKKEGDDSLPPESENAKSSMESQSEKKEGEPNAIFRVLPYWGGYFKQAAYRRLH
                     PQTFEWGKDELEIEEPTRESLNAQETKILSGKIHGNQTVALPLPYEWAVDTDNFDTDA
                     PRENAKIFKNQNGDWHLQIAGEGVFQYQLRIAPKQSVEIGGKFSESETNVGGNLDREL
                     QEKIQELKKENLPKMKLAREVVKFIRNNLVYSSGSAESVAAWRYYTENPEEFFQRIWE
                     QKKADCFVANTMAVRALAEAGLKTRFAGGYFVKGKNKDGAAVMHSGNGHGWLEVWDDL
                     SGRAVRLDATPKGDPNVDEQQQEKDLEGETGEGDYGEENNELMSEEKLKEKIKKMKEQ
                     QGKKEQERETASDPEEARFAELAECSPEQAREFLKALEKVREIKDGRGVPISELLKLE
                     WKKIITERKIETNDYRGPVRMDEGDNLEDPVSARIDILSKEFNPTGFEKLEKEEKIET
                     EFGGINIYFSFDLSGSMRESDGASGRSKADVQRDVALLFADSLMQCAYASRQQSENSD
                     LLPIKIMVTLASDTGKARLHLTDKWGPKEQWAFYSALNRPAKGGTPTHTTLKLIEKDF
                     DLEIADLKKKKIPKEKLPLHYTVEISDGAPDDFSQTEAMHRELKKKGMAIRSYCVGGV
                     SASVDAAEPIASFSQLPEILAEDIIEKFKKLNPGRIRQ"
     gene            11670..>12620
                     /locus_tag="VMQ48_02540"
     CDS             11670..>12620
                     /locus_tag="VMQ48_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08945.1"
                     /translation="MSFEKPENFQPYTLSPEEAKTMRPLTEEEREELGLGERKKEKKL
                     RPEIAGSRLPVTFRNPETGIDQSIEIDLENSLKEQKAFYKKNLNLEINESAVREIWNK
                     HHAEISREIETYGYDSILIIPNNLPEEEILNRDLVETMDEGAGKGNVAATWQSDNFKN
                     DGKSFAGVRNTYSPEYRLILTHSDQNIYENPAANPYLKVTLGKNIMDLTGLDEKEVQK
                     RIKNKQEIKVDFEAVIGGQKKQIQSDGGSLEEYLLQQAMHFKKTGHHLDEKGWTWLMK
                     SFSGSRVVFSRWAPDDRQLRVDAGGPGVAAGGLGLRLSRSF"
BASE COUNT         3740 a         2742 c         3076 g         3062 t
ORIGIN      
        1 atatcagaag cagcgtgacg tgaagttgga cgccgatgcc caaagcgatt ccagccagaa
       61 tcgcccaagt ccaggaaact ttttcccgct tttcaagaaa ttccaaaagc gacagcaaaa
      121 acaggatgac aaaaaatgga atagggttgg tattataagc gaaacgggaa tactggatga
      181 tataaaaaga aaccgcataa agtccggtca gggccagaga ccaattgccg ctgaagtacc
      241 gtttcagaaa gaaatagaaa agtggaatgg acaaaatagc aaagaacaaa tcgggatagg
      301 cggctgtttc cggatgcact ccaaaaaggt gaccggatat gatttggaaa taatagtata
      361 tcggccccac atgaaaccgg aatttgccat cctgcgagtg gctcatatcg gggcccaaaa
      421 gcggccaaga tccgcctttg tccaccactt tttccgtgat cgacaaatcc ctggcttggt
      481 cggactcaaa atcaagccag tttcgaaagt gataagaacg caaaaaaatg ccgacagcga
      541 atatggaaag aaataatatt attttccatt ttttattttt tatttttgca acaagtctag
      601 caggcattat attgaattta ctcgaaatat ctgataatta tctttggccg caagttaatt
      661 tcggagctgg taaatcgttt cctgcccgaa attttcagac gccgcaaact tgctactggt
      721 ctcatcactt cctccttcgg aactttggat atagaacgcc tgtttcccgg ggggaatatg
      781 atctacatcg ctggctatca gcaatttgaa ccggttctct cccgcaataa atttgagcgg
      841 tttgacacag ttatggctga acatgctcgt catgagatag gcctccctct gcccgcccgg
      901 cgtgtgagca atgatgtaat ttaccatagt ttccgtctct ccaagcacaa tatgatcctt
      961 tccgtttcgc gcgcgcgtga gcagttcttt ggcggcagtt tggatggaaa gatagttggc
     1021 tgctaaaacc agcgaataaa ttaggacaag aaacggcaga aatttctgga aattttttcg
     1081 ggcgaaataa tccaggataa atccaaggaa aaagaaaggc gcgaaaaaaa cgggaagaaa
     1141 atatctgtat tggcttgcta ctaccggaag gaatacgaca aaaaaacaag aaacgaataa
     1201 aagcgccagt cccaataaat atttcctttt ttcctctttt tctctgcgaa acttgtaaat
     1261 caaaaatcca tatccaaaca cggaaaataa aattccaaac aataaatttg cccaggaacc
     1321 gagactgatt tttctccatt gcgccgggtt ggtataggag aaattgcatt ggctgtcgcc
     1381 caaagaagac aatatatagg cattggcctg tacgtggcag ctgatatcca agggaatatt
     1441 gttgaggagc gttccattgc cgctcccgga gccactgctt tgaagggaaa tgaaaaaaat
     1501 cctagtattg gcaaaatttg tccttatttc gctcacaatt tggccggcat tcaggatgag
     1561 cgcgatcaat aaaaccgctc cccatttttt ccagtcttgc cggtattttc tcaaaagaaa
     1621 gaagaaaaca actgctgtca ccaacggcag cagcaccaag agaatagcat ggagctgaat
     1681 acccactccg agcgccagcc ccaaaaaaac tgcccatttc caactggttt tttctttgtg
     1741 cgctaaaaat tccagcaggg aaaacaaaaa cagcaaaaca aaaaacggaa tcaaattcgg
     1801 attcaatgcg gaatgggaaa agttcagcac ataaaaagaa accgcataaa gcccggtgag
     1861 cgccagcgtt atgttggtgc tgaaaaaacg cctgaagaag atatagaaga gcggaagagt
     1921 caagatatca aataacaaat cgggataagc cagcgcatcg ggccggttgc cgaacagttt
     1981 ggctgaagtg atttcgaaat aatagaacat cgaccctaaa tggtattgcc ctttgcgtcc
     2041 gccgtttcca gtgtggctca ttttgggtcc caaaagcggc cagggagcgt tgtgatccac
     2101 aacttttgtc acgatggaag catcgtttac ctgatcatcg ccgaaatcaa gccaatcgtg
     2161 aaagtgagtc gcgcgaagaa ataggccaat ggaaaaaatt gctatagcta tgataacggt
     2221 tattttcttt ttgttttttt tccaaaaact ttcttgatct cggctcatgg aagaaatact
     2281 tacaagtctt tcatattttc acagattatt cttttgctca tcgtcagaaa atatccagtc
     2341 ggatattcgt ataatacatg atataagcag aatatgaata attgatgaat aataggggat
     2401 tcacgagaaa ttcatttata tggtatagtt tctaagctat gaagatactt gttgttgagg
     2461 acaatgaaaa attggcaaaa tccctgaaaa agggcctgga acaggagggg catgcggccg
     2521 actatctcac tgatggagag gcgggacagc gccgattgga agtcagcgca gaagactatg
     2581 accttgtcat tcttgacttg atgcttccga aacgggacgg aatcgcggtt tgcaaaaact
     2641 ggcgcgacca aaacatcatg acgccggtga ttatgctgac cgcaaaagat gcggacgatg
     2701 acaaaatttt cggtttggac tccggcgctg atgattatct cataaaacct ttctcttttc
     2761 cagaactctt ggcgagaatc cgggcgcttc tgcgccggcc aaaaacgact ttgccgtccg
     2821 agctcaaaat caaagacctg gttctgaatg tctccacccg aaaagtcttt cgcggccaga
     2881 gggaagtcca tctcacgctg aaggagttcg cgatacttga atatctggtg aggcagccca
     2941 atcaagtcct gaaccgggaa caaatcatca gccatgtgtg ggattttgcc tttgactcat
     3001 tcagcaatct ggttgatgtg catatcaaaa atctgcgaaa gaaattagag aagaagaatg
     3061 atgaaaaatt tttggaaaca attcgaggca ttggttaccg gatcaaaggc tgacctattt
     3121 tcctcggcgc gtttgcgatt gacggcctat tacctcttga ttatgaccgg ggtgctggtt
     3181 attttcagcg cggtcctgta tttttctttt gcctatgtgt tgaaagacaa catcgatacg
     3241 caatttcccg atgttccaga ccaaggggat ctggccatcg tccaaacaat gagcgacgcg
     3301 aaagacggcg tagtggtgat cgacctcacc gctttcgctc ttacgattgt tttgggatat
     3361 agccttgcgg ggagaacctt gaggccgatt gaagatgtgc tggaacagca aaaggaattc
     3421 agcgccaatg cttcccatga gcttcgtacg cctctcgccg tgatgaaatc tgaaagtgaa
     3481 attgcgctaa acaaccccaa gacatcgacg gccgatttga gagcggtcat tgaaagtaat
     3541 cttgaagaaa tcaaccgaat gtcccaaatg acggaaaatc ttctcaagct ttcccgggtt
     3601 gaaacgaaaa aagaaaaaat atatttttcc gagatcgagc tgggggagtt tgtcagggag
     3661 gcagccaatc gaataagttc gcggacttct caaaaggggg tttccattga agtcgaaaaa
     3721 accgattccg gaaaaatttt gggcaatcgg gacgaccttg tctcagtggc ttccaatctc
     3781 atccaaaacg gagttgacta caacaagccg ggcggcgcgg tgcgcgtcag tgtcgagaat
     3841 agccggcagg aaatgcggct ggtggttgag gacaacggct tgggaataag cgaggatgat
     3901 ctgccgcata tcttcgaaag attctataag gcggacaaaa gccacagccg gaatttttca
     3961 aacgcgggaa tcgggctttc cattgtgaaa agaatcatcg acaagcacaa cggctcgatc
     4021 aaaattgaaa gcatccttgg aaaggggaca aaaattgtcg tttcttttcc ttcagccggc
     4081 gcctaagcta tcgcgcatag acatagaaga aatctttttc atagccgccc aaatattgct
     4141 ttgatccgtc ctgcaagtcc ttgattagtg tggtagaagt atattttccg gaaccggtcg
     4201 gatcgaagat attgaatctc acccagatcg gattgatatc cagctggatc gcattcacgg
     4261 cgccggcgga tttcaaggca gaagccaaag tgtcgggcgt caaattgttt ccgacggcga
     4321 aaagaagatt gccttctttt gtgaggccga tgcccgaccg ccaggtataa atatcgacag
     4381 ttcccgcggc caaacggccc caaagcgcct tgctgcgggg atcggtgacg gatatttgcc
     4441 cattggtgac gagcatcggg caattttggc gcacgaaagc gacattgtca ccaagcgatt
     4501 gtcccgtata gtcaaaaatt ttcaatgatc cgtcgttgta tccgaccaaa gtgcccaggt
     4561 catttttgag cggcaaatag gtcttgtctc cgacgatcat gccaaaggct ccgtccttat
     4621 actggaatcc gccatcaaag gctgccacca attttccact gccaatgata tctttgggaa
     4681 cgacacctgg tcccggtttt cccaccggac cgcccggttg tttcgtccca gccacactgg
     4741 ccaggcgcaa ggcatttgta tccatttgga cgatcgtggt gattgaatat gatcttgccg
     4801 ggtctgttcg aagaaaggta tatgccataa cttcttggtt tggaaaaagg cccaaaggtc
     4861 gattgcgcca aacgccttct ccatcaatag gcttgagact gtcatccact ttgagagggg
     4921 tgagattgag cgaccccccg gcaagatttt ctccggaacc ttggtcttca aaatgcggcg
     4981 ctatgtttga tccggaattg tgagtcagac gctggacgag atcggaagaa ttgaaaaata
     5041 ttttttccaa ataaaccacc cgattgtctc ccaaaagagg ccgcaagaca ttgtcagtga
     5101 gcacggccgc gccttttgtg ttgagcttca gaaaaagcac caggccgaca agcaaaaaaa
     5161 caagcactat ggcagcaaac ttgatccgtc ttaggattct ttttcccaaa gagtttcttt
     5221 tgccatacca gcctatcggc gcttttgagc tgtgcagact tcttcgcata atttagataa
     5281 agttttattt taggtataat acaggcaaca aatgaaaaaa gtatgaaggg aaattgggtt
     5341 tcatcgtttc ttcatttggt ctgattataa tcagaccagc taatatgtaa taagaactaa
     5401 tcgtgctaaa cttatgaagt acatgaagaa agaaaacatt attatcgcgg tcatagtcgt
     5461 cgtcatcgtg gtcgtcgggg cgtttgcgtt cttcaactcg aaaaaagcaa aaaaaacagc
     5521 caaagcaaaa acgcaaagtt cccaaaccca gacgcaaacc aagaccaagt ccaagaaaaa
     5581 cgctcccgcc aatggaacga accaaaacag cgcttccggt acggacaata cttccaatac
     5641 ccctaacact tccaacgcgg acacttccaa cagcaatccg acaagcaatt gacagctgat
     5701 caagaaaatc tgccttagct aattctgctc tgacacaaaa aatgaacagg aaaaaaatag
     5761 caatattaat attgatcgta ttggcgatta ttgttttgtc actgtttttt ttcagatccc
     5821 ctcaaaaaac aaaaaaaaca aatttacttc cggcgaatcc gcctacttcg ctcgatctgc
     5881 attccttcaa aggcgttata acgttgataa cggatgtggt gacaggaaac ataccggaag
     5941 tggccaaaaa ttcaaccaac caggcggctg ctccatcgac aatcagcaag acacaaggcg
     6001 gtttttcctt cgctatcatc ggcgacacaa aactttttga cgattccccg gccagcggaa
     6061 acttgcaaaa ggccgttggt tcgatctcaa aacaaaatgt tgatatggtt tttgctgtcg
     6121 gggatcttgt tttcaaatgc ggggatactt cgtcttgcga aaatagttat ggtgactgga
     6181 agacggcaat gtcgccgctt atgtccaaga catatgaagt tgtcggcaac catgaccggt
     6241 cgggcggaaa tcttgctgat gatgtttggc aaaaagaatt tgacttgccg atcaatggcc
     6301 cggcggattt tcagaagcaa acctattctt tcgactttca aaattcccat tttgtggttc
     6361 tcgataccga aaaaccacgg ggcggagtgg taaacttgga gcagcgcatc tggctcgagc
     6421 aggacttggc ggcgcacaag ctgcagcata cttttgtcct ctttcatgag ccggcttttc
     6481 cgaccagcca gttcaagaga aaaagtttgg atgtcgatac cagacaaagg gacgcgcttt
     6541 ggaaaatcat ggttcagaac aatgtgacgg ctgtgatcaa cggccacgag cattttttca
     6601 ccagaaaaaa agtcggaagc atttaccaat ttattgtcgg caataccgat gcgcccgaaa
     6661 acgccattcc aattccaaaa ctttccgact attcttatgt gggcaatata tatgccatcg
     6721 tcaccatttc cggagcaaag atgaatatgt ctttgtattc ggtcgacggc aagctgataa
     6781 attcatttga tttttagctg aaattgaaag aaaaatattt tttcttctaa actattttaa
     6841 aagcccggcg gtgccaggct tttagttttc ttctttatat tctctttgat attcccaata
     6901 catgcggatc caggagaatc tcggctcata tagagcaaag gaaaaatggt cgaagttgag
     6961 gtcggtggac gtcacaagga cggagcacac cggtcgatct ccgcaaaaaa gaaccttgag
     7021 ttctgccgtt gggccgccgc cggttttgcc gacgagcctt tctggataaa ggggatacag
     7081 ttcgatcttg aactcgagag gccagttggg atatgttttc tcgatccttt ctcgaaccat
     7141 tcgaaaaaaa tcctcaattg cttggtaggc aggagcctgc tcgcctgtgt tgcaggttat
     7201 cggcttcggc accttcaact ttctccaaag tttctttctt cttgccattt ggcaaccctc
     7261 cttatatgca tattttgcga tttttcagtt tggcatatcc taacagaata atttttaact
     7321 gtccattttg taaatttggc aaaaaagtcc caatagcata ttattagagg agaaataatc
     7381 ttgaaaaata aaaaaatggc tccaaaaaca aaaacgacaa aaacaaaaag caagccggct
     7441 atcaaaacag caatcaaaag caagtcgccg ttgaaaaaaa cagcggtcga aaaaaagccg
     7501 gcagttgaaa aaagccaaaa agacatcagt ttcaagctgt atatccttct ggccgtgctc
     7561 attgtggtcg gagcgctttt tttgcggacg tacaaactgg gcacgcttcc gccgggagtc
     7621 tatccggatg aatccgtcaa cgggattgac gcgatcacgg ccaacgccgc gcatcactgg
     7681 cagcttttct atgaaaacaa caacgggcgg gaaagtttgt tcatgaatct catcgccttg
     7741 ggcttcagtt tgttcggaat cagcgtggtc acgctgaaaa tgtggtcggt tttcttcggg
     7801 acgctgacgg ttctcgggat gattgtgctt ggcgaagaac ttttcgcgag ccggcgagcc
     7861 gggctcgttg ccggatttct gacagccact tctttttggg cgatcgattt cagccggatt
     7921 tcattccgcg cgataatgct gccttttgtc ctcgtgttca cggtattttt tctgatccgc
     7981 ggcattcaaa agaaaaaaat aatcgactat gtcttggccg ggttgttctt cggactgggt
     8041 cttcacacat acatcgcttt tcgcattgcg ccgctcattc tggtcgtact tctcgttgct
     8101 ttcattgcga accgcaaaag atttctggcc acccactgga aagaaatttc ggcatttttg
     8161 gtggccacta ttctcgtggc gttgcctatg ttgattgatt ttgttcataa tccggaacat
     8221 ttcagcggac gcacgggtca agtttctctt ttcactcccg aagtcaatca aggccatctt
     8281 cttcctatgc ttggcgtgag tattgccaaa tctttggcca aatacagttt cttcggcgat
     8341 caaaactggc ggcacaacct tccgccttgg ccggagctct ttcccacgat cggaatattt
     8401 tttctggccg gttttctcta tttcatctat gaatttttct atctggcttg gcgaagaatc
     8461 cgagcgggcg agagaagcga gcgcttgatt cttgtcgcac tgctcctttc ctggtttttc
     8521 gcgatgctta ttccggaagc catcacgaat gaagtgccgc atgccctcag aagtattggg
     8581 acaatgccgg tggcgatttt gatggctgta ttcgcgattg agggcgcctg gaaatgggcc
     8641 gacaacaaag agaagctcaa aaaatacaaa gaggctatct ggacgctcat gatcgttctc
     8701 attgttggga gtggaatttg gtcagtgcgg atgtattttg tcgattgggg agggaatccg
     8761 aatgtccccg gggcttttga cgagggctat ttgaatatgt cgaactatat caacagcttg
     8821 ccagcgagta ccccgaaata tatcgttgac aacgggccgg gaatcaaaat gcaagacgga
     8881 cttcagactt cggcggaagt gataaaattg ttcacctatc aaaaagcgga taatgtcatg
     8941 tttgtcgaac cggatgtcga cctaagtacg ctcaaaactc cgtcgaaaat attcctgatg
     9001 aacgcggatg atggttttgt gagctatctt cgccagtctt ttcctgcaag ccgggtggaa
     9061 aaaatcgatg tccgggaagg aatggacaca ggcttcacga tgattgacgt aaaatagttt
     9121 tatactattc ttgaaaaagt gatttggaaa aatatgccca gactggagaa aaaaattgaa
     9181 cagccaagca aaaaaccgga atttgaattg aaagatctgg caacagccga ggaatatttg
     9241 gattggaaaa aaaatgttcc ggtcggagta aggcagattg tggaaaccgt cataagaggc
     9301 aataaattgc aacccagtca gaccggtctg gcgctctcgg atcaatttct gagtgcgttg
     9361 ggagaatcgt tggcatcaga cgaacaagtt gatttgtccg gagcggacaa gcgagttttc
     9421 aatttgctgg atccgttgag acgcattgaa accaaaggcg ggcatttgaa tgtttttgaa
     9481 acattgcgca atccggaagc ttcttggaat ctgaagcgaa aaatttttga aatgcagatc
     9541 aagccggcac tggattggct ggccgagaga gatctggaaa aaatagcgga ggaggagagg
     9601 cgagctttgg aaagcgagca aacgcctcaa gaaaaacaac ccgaccggaa aaaagaagga
     9661 gacgacagtt tgccgcccga aagtgagaat gccaaatcct caatggaatc tcaatcggaa
     9721 aaaaaagaag gagaaccgaa cgcgattttt cgcgtcttgc catattgggg cggctatttc
     9781 aagcaagccg cttatcggcg gctccatccg caaacttttg agtggggaaa agacgaattg
     9841 gaaatcgaag agccgacgcg ggaaagtctg aatgcgcagg aaacaaaaat tttatccggc
     9901 aaaatccatg gcaatcaaac tgtggctttg ccgctgccgt atgagtgggc ggtggacacg
     9961 gataatttcg acacggatgc gccccgagag aatgccaaaa ttttcaaaaa tcaaaacgga
    10021 gattggcatt tgcaaatcgc cggggaaggt gtttttcaat atcagttgcg catagcgccc
    10081 aaacaatccg ttgaaatcgg gggaaaattc agcgaatcag agacgaatgt tggaggaaat
    10141 cttgatcggg agctccagga aaaaattcaa gaactgaaaa aagaaaattt gcccaagatg
    10201 aaactggcga gagaagtcgt aaaatttatc cgcaacaatt tggtttattc gagcgggagt
    10261 gcggaaagcg tggcggcctg gcgatattat acggaaaatc cggaagaatt ttttcaaaga
    10321 atatgggagc aaaaaaaggc ggattgtttt gtggccaaca caatggccgt gcgcgctttg
    10381 gcggaagccg gcctcaaaac cagatttgcg gggggctatt tcgtcaaagg aaaaaacaag
    10441 gacggcgcgg cggttatgca ttcgggaaac ggccacggct ggctggaagt ctgggatgat
    10501 ttgagcgggc gagcggtgcg gttggacgcg acgcccaaag gcgatcccaa tgtggatgag
    10561 cagcagcaag agaaagattt ggagggtgaa accggcgaag gcgactatgg tgaagaaaat
    10621 aatgaattga tgtcggaaga aaagttgaaa gagaaaatca aaaaaatgaa ggagcaacaa
    10681 ggaaaaaagg agcaagaaag ggaaactgcg tctgatccgg aggaagcgag attcgcggaa
    10741 ttggccgaat gcagtccgga acaggcaagg gaatttctga aagctttgga aaaagtgagg
    10801 gaaatcaaag acgggcgcgg tgttccgatc agcgaactgc tgaaactgga atggaaaaaa
    10861 ataattacgg aaagaaaaat tgaaaccaat gattatcgcg gaccggtcag gatggacgaa
    10921 ggggacaatt tggaggatcc ggtgagcgcc agaatcgata tattgtccaa agaattcaat
    10981 ccgaccggct ttgagaaact ggaaaaagaa gaaaaaatcg aaacggaatt cgggggcatc
    11041 aacatttatt tctcttttga tctgtcggga tcaatgagag aatcggacgg ggcgagcggc
    11101 cggagcaagg cggacgtgca aagagatgtt gcgctgctgt ttgcggacag cctgatgcaa
    11161 tgcgcctacg cctcccgcca gcaaagcgaa aattcggatt tactgccaat caaaattatg
    11221 gtaacattgg cttcagatac gggaaaagcc agactgcatc tgactgacaa atgggggccg
    11281 aaggaacagt gggcgtttta ttccgcgctc aatcggccgg ccaagggagg cacgccgacc
    11341 cacaccacgc tcaagctgat tgaaaaagat tttgatttgg aaatagccga tctgaaaaag
    11401 aaaaaaattc caaaagaaaa actgcccttg cattataccg ttgagatttc cgacggcgct
    11461 ccggatgatt tcagtcaaac tgaggctatg cacagggaat tgaagaagaa gggcatggct
    11521 atccgaagct attgcgtcgg gggagtctca gcgagcgttg acgcggccga gccgatcgcc
    11581 tcattttctc aattgcccga gattttggct gaagacatca ttgaaaaatt caaaaaactt
    11641 aatcccggaa gaataaggca ataaaaacta tgtcattcga aaaaccggaa aattttcaac
    11701 catacaccct ttcgccggaa gaggcaaaaa caatgcgtcc gttgacagaa gaagagcggg
    11761 aagagttggg tcttggtgaa agaaaaaaag aaaaaaaact ccggccggaa atagccggtt
    11821 cgcgacttcc agtgactttt cggaatcccg aaacaggaat cgatcagtcg attgaaatcg
    11881 atctggaaaa ttccctgaaa gagcaaaaag ctttttacaa aaaaaacctc aatctcgaaa
    11941 tcaacgaatc cgcagtcagg gaaatctgga acaaacatca tgcggaaatc agccgtgaaa
    12001 tcgagacata cggatatgat tccattctga tcatcccgaa taacttgccc gaagaagaaa
    12061 ttctcaatcg cgatctggtt gaaacgatgg atgagggcgc gggcaaaggc aacgttgccg
    12121 ccacatggca aagtgacaac tttaaaaatg acgggaaatc tttcgcggga gtcagaaata
    12181 cgtattctcc cgaatatcgt ctcattctga cccattctga ccagaatatc tatgaaaatc
    12241 cggcggccaa cccgtatctc aaggtgactc ttggaaaaaa tatcatggat ctgacaggcc
    12301 ttgacgagaa ggaagtccaa aaaagaatca aaaacaagca ggagataaaa gttgattttg
    12361 aggctgttat tggcgggcaa aaaaaacaga ttcaatccga tggaggatcc ttggaggaat
    12421 atctgttgca acaagctatg cacttcaaaa aaacagggca tcatctggat gaaaagggct
    12481 ggacatggct catgaagtct ttctccggtt cccgggtcgt gttctcgcgc tgggctccgg
    12541 acgatcggca gctgcgtgtc gatgccggtg gtcccggcgt tgcggctggc ggtcttggcc
    12601 tccgcctctc ccgtagtttc
//
LOCUS       DATKGS010000102         3873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_155306, whole genome shotgun sequence.
ACCESSION   DATKGS010000102 DATKGS010000000
VERSION     DATKGS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155306"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VMQ48_02545"
     CDS             <1..610
                     /locus_tag="VMQ48_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUD08946.1"
                     /translation="GGIQHFSLQLLTSIMLFNFFSEGTMAGLNSLMSKASLVTKIYVP
                     RWTIVIASTINASLIFAMNLIIIAVFFTWYGYWPGIPAILMFVYFIILTFILILSFSF
                     IAAPLYVKFRDLSQIWEVLIQALFYGSPIVYPLQIIPVKLQRVLLANPIGYIIHFTKQ
                     ALTEHHYAPFWQNAIFSIIIFALFFVSVLIFRKMEPKIAENI"
     gene            621..2486
                     /locus_tag="VMQ48_02550"
     CDS             621..2486
                     /locus_tag="VMQ48_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08947.1"
                     /translation="MLKQKYKLAAGVICLLFFSLALFSSAGFSWRNLKYSGNGIIADE
                     IPHIATGYYYLKTHRYFINPEHPPLVKDVAGLGESVVHPAFPDITDVPSQPDNFQRYS
                     YPFAGNDFPSFLERSNTQAYFGQMYLFNSQNNPDLIAFWGRLAVILVNTLLLFALFLI
                     ISKIWNPRAALIGLFFIAVSQFSIAHGSFVVIDFMSGLLSIIATGAFAIFWKKYAEGK
                     ANAWYFLAAAAGLFLAEVSKFSSVILFPAFFLGGLVFIIAAKKSWKEFFKYIGIFTLM
                     MIVTLLLIAAFYVLHVYKMDNGAMVQQLWDNFPYGTLPQSLMAIFAPMVSLNILTKGL
                     VEYFNGVLMVMGRMSGSSQITYFMGHVYGNEGAGPSYFPVLYFTKLSLGFLLFNLVAL
                     ILIIKKIFTDKQKISVRWRNFVSNPFALLLFIFAYLYMVETLSSTFQIGLRHIMPVIM
                     AVAVLTGRGVDIFWDQKIWKKISLKHVFMAVGSLMVVSVLCAFPFYLSYYNALGGGTE
                     NGYKIATDSNYDWGGSDVRRLAEWMKGNNAHSIYTDFFADVELPYYLGDGQHNFNIED
                     GFMPPPGSLIAVSNYKYMNNIANDKLRPEQKYTILKNDLIARVGPTIMVFKVPGK"
     gene            2488..>3873
                     /locus_tag="VMQ48_02555"
     CDS             2488..>3873
                     /locus_tag="VMQ48_02555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUD08948.1"
                     /translation="MEKLKNFFAKYERHWIWLILVLAAALRIYQINDRDFWYDEAFTG
                     IAVKERFHDMMTMIINDVHPPLYYWSVKLFASFFNYSVLGIRMFSAVFGVLGVWAIYL
                     FTKELFDRRTALWAALIIAISPFAVQYSQEARMYVMLVFLITIAAYFFVKALKTDRTL
                     YFVWWGIFTGLACLTHYMGIIFASVYLPAFIAWKIIGGRIYEKQIGWQMVKNFLPSKK
                     LLIGYGAAFLIFLPWLPIFKHHLLNQGNNLSWIVPASFGDIFWNLQMFVFGTPKGEMS
                     AGMPNPNEFYGVANNTALTLLVIFFAGITIYLFKKRKDKEKISALLILSVGFMFIVYL
                     LSLLEQRYFVARYLMPAAYFTFVYIGLWLATVRFRTAIYAMAIYITLMFTFQPYYYSQ
                     GWNLLDQNLSKYKNNNFYILNSFDYVIGKYYLGANHLTLYNIDWPQYNPDYWAAIGPS
                     LKRTENINDLRN"
BASE COUNT          887 a          957 c          879 g         1150 t
ORIGIN      
        1 ggggggcatc cagcattttt ctctccagct gctcacttcc atcatgctct tcaacttttt
       61 ttccgagggg acgatggcgg ggctcaattc gctgatgagc aaagcttcat tggtgacgaa
      121 aatatatgtg ccgcgctgga caatcgtgat cgcttcaacg atcaacgcgt ctctcatctt
      181 cgcgatgaac ctcatcatca tcgcggtttt tttcacttgg tacggatatt ggccggggat
      241 tcccgccatt ctcatgtttg tgtatttcat catccttact tttatcttaa ttttgtcttt
      301 ttccttcatc gccgcgccgc tctatgtgaa attccgtgat ctttctcaga tatgggaagt
      361 gctcatccag gccttgttct atggctcgcc gattgtttat ccacttcaaa ttattccagt
      421 caagctgcaa agggtgcttt tggccaatcc aatcggctac atcattcatt ttacaaaaca
      481 agctcttaca gagcatcact atgcgccgtt ttggcaaaac gcgatttttt caatcattat
      541 attcgctttg tttttcgtga gtgtgcttat cttccggaaa atggagccaa agatcgcaga
      601 aaatatttaa aaacaaaaaa atgctaaaac aaaaatacaa actggctgca ggtgtaattt
      661 gcctcctgtt tttttcgctc gctctttttt ctagtgccgg gttttcgtgg cgcaatttga
      721 aatattccgg caacgggatt attgccgacg agattccgca catcgcgacc ggatactact
      781 atctcaaaac gcaccgctac ttcatcaatc ccgaacatcc gccactcgtg aaggatgtcg
      841 ccgggttggg cgaaagcgtc gtccatcccg cttttcccga catcaccgat gtgccgagcc
      901 agccggacaa tttccagcgc tacagctatc ccttcgccgg aaacgatttt ccatcttttc
      961 tggaaaggtc caatacgcaa gcctatttcg gccagatgta tcttttcaat tcccaaaaca
     1021 accccgatct gatcgctttt tggggacggc tggcggtcat tctcgtcaat acactgctgc
     1081 tgtttgcgct gtttctcatc atttccaaaa tatggaatcc cagagcggcc ttgatcggac
     1141 tttttttcat cgccgtttcg caattcagca tcgcgcacgg atcattcgtc gtgattgatt
     1201 tcatgtccgg gcttctttcc atcattgcaa cgggcgcctt tgcgattttt tggaaaaaat
     1261 atgccgaggg aaaagccaac gcctggtatt tcttggccgc cgccgccggt ttgtttctgg
     1321 cggaagtttc caaattttct tcagtcattt tgtttcccgc gtttttcctg ggcggattgg
     1381 ttttcatcat cgccgcaaaa aaatcctgga aagaattttt caaatatatc gggattttca
     1441 cgcttatgat gatcgtgacg cttttactga tagcggcttt ttatgttttg cacgtctaca
     1501 aaatggacaa cggcgccatg gttcagcagc tgtgggacaa ttttccctat ggcacccttc
     1561 cgcagtcgct catggcaatt ttcgcgccga tggtttcact caacatcctg accaaaggat
     1621 tggttgaata tttcaacgga gtgctgatgg tcatggggcg gatgtccgga tcttcccaga
     1681 tcacctattt catgggtcat gtgtatggaa acgaaggggc gggtccgtca tattttccgg
     1741 tgctgtattt cacgaaactt tctctcggat tcttgctttt caacttggtg gcattgatcc
     1801 tgatcatcaa aaagattttc acggacaaac aaaaaatttc cgtccgatgg cgcaattttg
     1861 tttccaatcc gtttgcactt ttgcttttca ttttcgcgta tttgtatatg gtcgagaccc
     1921 tttcttcgac ttttcagatt ggcctccggc acatcatgcc ggtcatcatg gcggtggcgg
     1981 tgctgaccgg aaggggagtg gatattttct gggaccaaaa aatttggaaa aaaatcagcc
     2041 tcaaacacgt atttatggca gtcgggagcc tgatggtcgt ctcggttttg tgcgcttttc
     2101 ctttctattt gagctattac aacgctctcg gcgggggaac ggaaaacgga tacaaaatcg
     2161 ccaccgattc gaactatgac tggggcgggt cggacgtgag acggctggcc gaatggatga
     2221 agggcaacaa cgcgcattcg atttatactg atttttttgc cgatgtcgag ctgccgtact
     2281 atctcggtga cggccagcac aatttcaata tcgaagacgg cttcatgccg ccgccggggt
     2341 cgctcattgc tgtttcgaac tacaaatata tgaacaacat cgccaatgac aagttgcgtc
     2401 cggagcaaaa atataccata ctgaagaatg atttgatcgc ccgggttggc ccgactataa
     2461 tggtcttcaa ggtgccggga aaataatatg gaaaagctga aaaacttttt tgcgaaatac
     2521 gaaagacatt ggatttggct gattttggtt ttggccgcgg cgcttcgcat ctatcagatc
     2581 aatgaccgtg atttttggta tgacgaggct ttcaccggca tcgccgtgaa agaaagattt
     2641 cacgatatga tgacgatgat catcaacgac gtccatccgc cgctctacta ttggtctgta
     2701 aagctgttcg cctcgttttt caattatagc gttctcggca tccggatgtt ttcggccgtt
     2761 ttcggcgtgc tgggcgtctg ggccatctat ctcttcacga aagagctgtt cgaccgccgg
     2821 acggcgctgt gggccgccct catcatcgca atttcgccgt ttgccgtcca atattcgcaa
     2881 gaagcccgga tgtatgtgat gcttgttttt ctcatcacca ttgccgccta tttcttcgtc
     2941 aaagctctca aaaccgaccg gacgctgtat ttcgtctggt gggggatttt caccggtctg
     3001 gcctgtctca cgcactatat gggaatcatt tttgcctctg tctatctgcc ggcgttcatc
     3061 gcctggaaaa taatcggcgg aaggatatat gaaaaacaaa tcggctggca gatggtcaaa
     3121 aattttctgc cgagcaaaaa attgctgatc ggatatggcg cggccttcct gattttcttg
     3181 ccttggctcc ctattttcaa gcatcatctt ctcaaccagg gaaacaattt gagctggatc
     3241 gtgccagcga gtttcgggga tattttttgg aatctccaga tgttcgtttt cgggacgccc
     3301 aaaggcgaaa tgtcagccgg gatgcccaac ccgaacgaat tctatggcgt ggccaacaac
     3361 acggcgctca cgttactcgt catttttttt gccggaatta cgatatattt gttcaaaaaa
     3421 aggaaagaca aagaaaaaat ttcggccctc ctcatccttt cagtcggctt tatgttcatc
     3481 gtctatctcc tctctcttct cgaacaaaga tatttcgtgg cgcgctatct gatgccggcg
     3541 gcctacttca cttttgtcta tatcgggctt tggttggcga ctgtgcgatt tcgcaccgcg
     3601 atctatgcga tggcgatcta tatcacgctg atgttcacgt ttcagccgta ctactattcc
     3661 caaggctgga acctgctcga tcaaaacctt tcgaaataca agaacaacaa tttctatatt
     3721 ttgaattcat ttgattatgt catcgggaaa tactatctcg gcgccaacca cctgacgctc
     3781 tacaacatcg attggccgca gtacaacccg gactattggg cggcgatcgg cccgagtctg
     3841 aagcgaaccg aaaacatcaa tgatttgcgg aac
//
LOCUS       DATKGS010000103         7077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_12668, whole genome shotgun sequence.
ACCESSION   DATKGS010000103 DATKGS010000000
VERSION     DATKGS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12668"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VMQ48_02560"
     CDS             <1..431
                     /locus_tag="VMQ48_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08949.1"
                     /translation="VEAPVKKMQPILQDEPKLRNPSVEEKNRYSVPLQEIKFKKKKDI
                     FSPDFAGEERVAAPMFSRFAFAMSFAVILLLVAASGYFVWSGGLLARGQVAGASDERN
                     AGFSGITSGGEYVLTSAGDKMKQSLSISRVMVQAAEEKSK"
     gene            476..1024
                     /locus_tag="VMQ48_02565"
     CDS             476..1024
                     /locus_tag="VMQ48_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUD08950.1"
                     /translation="MAMWVLLGIIVVLVIWAVAVYNGLIRLKNRVDEAWSDIDVQLKR
                     RYDLIPNLVNTVKGYASHEKEVFEKVTEARTRAMGAQSAADKAQAENALSQTLKSLFA
                     VAEAYPDLKANQNFLELQRELTDTEDKIQASRRFYNGNVRDFNTKIQVFPNNVFAGML
                     GFSAREFFEAAEGEKEPVKVEF"
     gene            1087..1983
                     /gene="htpX"
                     /locus_tag="VMQ48_02570"
     CDS             1087..1983
                     /gene="htpX"
                     /locus_tag="VMQ48_02570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HUD08951.1"
                     /translation="MATIYDQADKNTHLTWIYILGFLVFVIGIGYVFAGAMNNYAILV
                     IAVLFSVVMSFASYWWSDKIVLAMSGAKEVTRENAREIYNIVENLCITAGLPVPKIYV
                     IQDSSPNAFATGRDPEHAVVCLTTGIVEKLDKPELEGVIAHELSHIGNRDILLSTVIV
                     VLVGFVAILAGFFRRWSFFGGGRRSNDNEGGQLGLILMIVAIILSILAPIAAMLIQLA
                     ISRKREYLADADGALLTRYPEGLARALEKISADPTPMKSANQATAHLFIDNPFKGKKV
                     SSLFQTHPPIAERVKRLRGMDV"
     gene            2017..2391
                     /locus_tag="VMQ48_02575"
     CDS             2017..2391
                     /locus_tag="VMQ48_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08952.1"
                     /translation="MQFKIPKNTEKYEWTQHAAFKMKQYGLTAQRILRVVKNPQRTEE
                     GIVKNTIAVMQPASLRTKDGKRVWSQEIWAMYQIKGKSKNEKGKTFGMAKIRLISAWR
                     YPGVSPKRDPIPEEILRELNLG"
     gene            2480..2821
                     /locus_tag="VMQ48_02580"
     CDS             2480..2821
                     /locus_tag="VMQ48_02580"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HUD08953.1"
                     /translation="MTEEIKKADTSDAEKNKAMGIVGYIIPILFFIPLISDAKNSPFA
                     KFHANQQLNLLLAAIVVNIVGGIIPFIGWFIILPLGTIFLLVVAIMGIINAAKGEMKE
                     LPLIGSFKLIS"
     gene            2898..4373
                     /gene="pyk"
                     /locus_tag="VMQ48_02585"
     CDS             2898..4373
                     /gene="pyk"
                     /locus_tag="VMQ48_02585"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HUD08954.1"
                     /translation="MKRTKIICTIGPASESEKILSELVKKGMNVARLNFSHGNFSSHK
                     KAIKTIRKISEKLQIPVGIMADLQGPRIRVSNVAELAVSKGEIIEVKEALLPAKSNPL
                     KKIINKLRITKDANKQKTLKLDLAEVVRMVKTGSSILIEDGLIKLKVLKRTKDHLVCR
                     VANSGTIKPRKGVNIPGISARLGAITARDKEVLDFALGQNVDFVAMSFVRTAEEIEYL
                     KKLIKQKSRRKTGLAQVIAKIERKEAIKNFDKILAATDGVMIARGDLGIEMPQADLPI
                     LQKEICAKCLRVGKPVIVATQMLDSMIRNPRPTRAEVTDVSNAVIDHADALMLSGETA
                     SGKYPVEAVHTMTQIIRRTEKSPFDDLDHGFLGDQKESVSAAIAQSAHELLKDSRAKA
                     IVVASISGLTARMITRHRPEQKIFVMTNNEKTHNQLALVWGVNSFVLPDCKDLDELID
                     KSIEVIRKNKLLKKKDKVVIVAGRPHVKKEHMSLVKVEEIN"
     gene            4387..6816
                     /gene="ppsA"
                     /locus_tag="VMQ48_02590"
     CDS             4387..6816
                     /gene="ppsA"
                     /locus_tag="VMQ48_02590"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HUD08955.1"
                     /translation="MKQKYILWFKETGIKDIELVGGKNASLGEMFQNLKKKGIRVPDG
                     FSITAEAFRYFITQNKLDGEIKNILKGLDTGNMRNLAERGYRVRQAILSAEFPVDLRN
                     QIIEAYSKLSKAYDPRKSFRINWNNTDHTNHFAGGVDVAVRSSATAEDLVDASFAGQQ
                     ESYLNVTGEYQLLESIKKCFSSLFTDRAISYRVDKGFDQLSIALSVGVQKMVRSDEAA
                     SGVMFTIDTESGFSDAIIINGSYGLGENIVKGKVNPDEFIVHKPTLKQGFKSIVGKKM
                     GSKETKLVYSVGGTSTTQNAIVSQEDRRRFCLTDDEALELARWGLLIEEHYKKPMDIE
                     WAKDGRDGKLYIVQARPETVQSQKKKNVLEEYILKSKSKILCVGAAVGGKIGAGKANV
                     IKDVAQIADFQKGEVLVTEMTDPDWEPIMKIASAIVTNSGGRTSHAAIVSRELGIPCI
                     VGTGNATAAIKGKKPVTVSCAEGEEGKVYEGILKFEIKTTNLKNLKKTKTKIMMNVGN
                     PDQAFDLSFIPNDGVGLAREEFIITEYIKIHPMALVHFDRVKDKDEKKKINEMTQGYK
                     SKEDFFVEKLAEGIGRICAAFWPKDVIVRMSDFKTNEYATLIGGKVFEPAEENPMIGW
                     RGASRYYDEDYKEGFRLECLAFEKVREVMGLTNLKAMIPFCRTVEEGKKVLVEMEKYG
                     LERGKNGLQVYVMCEIPSNVILAKEFSKIFDGFSIGSNDLTQLTLGVDRDSAKVAHVY
                     DERNEAVKELIRQVIEKAHKARRKVGICGQAPSDFLDFAKFLVEEKIDSISLNPDTVI
                     KTTMEIGKMEK"
     gene            6899..6983
                     /locus_tag="VMQ48_02595"
     tRNA            6899..6983
                     /locus_tag="VMQ48_02595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6933..6935,aa:Ser,seq:gga)
BASE COUNT         2055 a         1502 c         1893 g         1627 t
ORIGIN      
        1 gggtagaggc tccggtaaaa aaaatgcagc cgattttgca ggacgaacca aaattgagga
       61 atccttctgt tgaggaaaaa aacagatact cggttccttt gcaagaaata aaatttaaga
      121 aaaaaaagga tatattttct cccgattttg cgggtgagga aagggtggct gcgccgatgt
      181 tttcgcgttt tgcttttgcg atgagttttg ctgtgatact tcttctggtg gcggcatccg
      241 gatatttcgt ctggtccggc ggtttgctcg cgagagggca agtggcgggg gcgagtgatg
      301 aaagaaacgc cggattttcc ggtattacat ccggaggcga atatgtcctc accagcgcgg
      361 gggacaaaat gaaacagtcg ctttccatat cgagagtgat ggtgcaagcg gcggaggaga
      421 aatcgaagta gggtggttta gtaaattagg gaaataattt tttaagaata aaattatggc
      481 aatgtgggta ctattgggaa taatcgtagt tcttgtgatt tgggcggtgg cggtgtacaa
      541 cgggctgatc cgactcaaga accgggtcga cgaagcgtgg tcggatatcg acgtccagct
      601 caagcggcgc tatgatctca ttccgaacct ggtgaatacg gtgaagggct acgcctctca
      661 cgaaaaagag gtctttgaaa aagtgacgga agcgcggacg cgggcgatgg gtgcgcaatc
      721 agcagcagac aaggcgcagg cggaaaacgc cctctcgcaa actctcaaaa gtttgtttgc
      781 ggtggcggaa gcctaccccg atctcaaagc caatcaaaac tttttggaac tgcaacgcga
      841 actcaccgac acggaagaca agatccaggc gtcacgccgg ttctacaacg gcaatgtccg
      901 cgacttcaat acgaaaatac aggtttttcc aaacaatgtc tttgccggca tgctgggatt
      961 ttctgctcgt gagttttttg aagcggccga aggagagaaa gagccggtca aagtcgaatt
     1021 ctaaactttc tttttgtgaa aactaatcac gattaaacta gtaatattac tagaacattt
     1081 tgctatatgg caactatcta tgatcaagcc gacaaaaaca ctcatcttac ctggatatac
     1141 atcctgggtt ttttggtttt tgtcattggg atcggctatg tctttgcggg ggcgatgaac
     1201 aattatgcaa tcttggtgat cgcggtgctt ttcagcgtgg tgatgagctt tgcttcgtat
     1261 tggtggagcg acaagatagt gctcgcgatg agcggagcca aggaggtgac acgcgagaat
     1321 gcccgggaaa tatacaacat cgtcgaaaat ctttgcatca cggccggtct gccggtgcca
     1381 aaaatatatg ttatccagga ttcctcgccc aacgccttcg cgactggacg cgatccggag
     1441 catgcggtgg tgtgcctcac gacggggatc gtggaaaaac tcgacaaacc cgaacttgaa
     1501 ggggtgattg cgcacgagct ttcacacatc ggcaatcggg atattctgct ttcgacggtc
     1561 atcgttgttt tggtcggctt cgtggcgatc ctcgccggtt ttttccgccg ctggagcttt
     1621 tttggcggag gaagaaggtc gaatgacaat gaagggggac aactcgggct catattgatg
     1681 atagtcgcca tcattctctc cattctcgcg cccattgcgg cgatgctcat ccagctggcg
     1741 atctcgcgca agcgcgaata cttggccgac gccgacggtg cgcttctcac cagatatccg
     1801 gaaggcctgg cccgcgcgct cgagaaaatt tccgccgatc cgacgccgat gaaaagcgcc
     1861 aatcaggcga cggcgcatct tttcatcgat aatcccttca aaggcaaaaa agtttcgagt
     1921 cttttccaaa cccacccgcc catcgctgag cgcgtgaaac gtttgcgagg gatggatgtg
     1981 taatttttcc caaggtgatg ccttgggaat ttttttatgc aattcaagat tccgaaaaat
     2041 acggaaaaat atgagtggac gcagcatgcc gcgttcaaaa tgaaacaata cggcctcacg
     2101 gctcaaagaa ttttgcgcgt ggtgaaaaat ccgcaaagga cggaagaggg aattgtgaaa
     2161 aatacgattg ccgtgatgca gcccgcctct cttcgcacca aagacggaaa aagagtctgg
     2221 agccaggaaa tttgggcgat gtaccagata aaaggaaaaa gtaaaaatga aaaaggaaaa
     2281 acatttggga tggcaaaaat tcgtttgatc agtgcgtggc ggtatccagg tgtgagtcca
     2341 aaaagagacc ccattccaga ggaaatcttg cgggaattaa atttaggatg atagtttgtc
     2401 tttttcggcc agattgggta ttataaaacc agtagaaaaa cacataaaaa atctctaaaa
     2461 cagaaagcga ggtgacaata tgaccgaaga aataaaaaaa gcggatacat ccgatgcgga
     2521 aaaaaacaaa gcgatgggaa tcgtgggata tatcattccg atcctgtttt tcatccccct
     2581 gatttccgac gcaaaaaaca gtccattcgc gaaattccac gccaaccagc agctgaatct
     2641 tctgcttgcg gcaatcgttg tgaacattgt tggcgggatc atcccgttca ttggctggtt
     2701 tatcattctt ccgcttggaa caatcttttt gctcgtggtg gctattatgg gcatcatcaa
     2761 cgcggcgaaa ggcgaaatga aagaattgcc gctcatcggc agcttcaagt tgatcagcta
     2821 aagtgctatt gcgcaatagt atttcaaagg caggcaaact attttggttt gtctgttttt
     2881 tgttgtataa tacttgtatg aagcgaacga aaataatttg caccatcgga ccggcgtcgg
     2941 aaagcgaaaa aatactttcc gagttggtca aaaaggggat gaatgtcgcc cggctcaact
     3001 tttcgcacgg gaatttttct tcacacaaga aagcgatcaa gacgatccgg aaaataagcg
     3061 agaaattgca aattcccgtc ggcataatgg ccgatctcca aggccccagg atcagggtga
     3121 gtaatgttgc agagcttgcg gtttcgaaag gcgaaatcat cgaagtgaaa gaggcgctgc
     3181 ttccggcaaa atccaatcct ttgaaaaaaa taatcaacaa attgagaatt acaaaagacg
     3241 cgaacaagca aaaaacgctc aaattggatc ttgcggaggt ggtgaggatg gtgaagacag
     3301 ggtcgagcat actgattgaa gatggactca tcaaattgaa agttttgaag cggacaaagg
     3361 atcatcttgt gtgccgtgta gcgaacagtg ggacgattaa accgagaaag ggagtgaata
     3421 ttccgggaat ttccgcgcgt ctgggagcaa tcaccgcgcg cgacaaagaa gttcttgatt
     3481 ttgccctagg tcaaaatgtc gatttcgtcg cgatgtcttt tgtgcgaacg gccgaagaga
     3541 ttgaatatct caaaaaactg atcaagcaaa aatccagaag gaaaaccggt ctcgcccagg
     3601 tgattgccaa gatcgaaaga aaagaagcca tcaaaaattt cgacaaaatt ttggcggcga
     3661 cggatggagt gatgatcgcg cggggcgatc ttgggatcga aatgccgcaa gcggacctcc
     3721 ccattctcca gaaagaaatt tgcgccaaat gtttgcgggt gggcaaaccg gtgatcgtcg
     3781 cgacccagat gcttgattcg atgattcgca atccccggcc gacccgggcc gaggtgacag
     3841 acgtttccaa cgcggtgatc gaccacgcgg acgccctgat gctttccggg gaaactgcga
     3901 gcggaaaata tccggttgaa gcggtgcata cgatgaccca aatcatccgg cggacggaaa
     3961 aatcaccttt cgatgatctt gaccatggat ttctgggaga ccagaaggaa tccgtctcgg
     4021 ccgcaattgc gcaatcggcg catgaactct tgaaagacag ccgggcaaag gcgattgtcg
     4081 tggcaagcat ttctggcctt acagcccgga tgattacccg ccatcggccg gagcagaaaa
     4141 ttttcgtgat gacgaacaat gaaaaaacgc acaaccaatt ggcgctcgtt tggggtgtca
     4201 acagtttcgt tttgccggat tgcaaagacc tggacgagct tatcgacaag tcgattgaag
     4261 ttataagaaa aaataaattg ttgaaaaaga aagacaaagt ggtgattgtg gccggccgtc
     4321 cgcatgtcaa aaaagagcac atgagcttag taaaagtgga agaaataaat taaaaataaa
     4381 ataaatatga aacaaaagta cattctttgg ttcaaagaaa ccgggatcaa ggacatcgaa
     4441 cttgtcggcg ggaagaacgc gtcgcttggc gagatgttcc agaatctaaa aaagaaaggc
     4501 attcgggtgc cggacggatt ttcgatcacg gcggaagctt ttcgctactt catcacccaa
     4561 aataagctag acggggaaat aaaaaatatt ctaaaagggc ttgatacggg caatatgcgc
     4621 aatcttgccg agcgggggta tcgggttcgc caagccattc tctcggccga atttccggta
     4681 gatctgcgca atcagatcat tgaggcctat tcgaaacttt cgaaagccta cgacccgcgc
     4741 aaaagttttc gaatcaattg gaacaatacc gaccacacga accatttcgc gggcggagtg
     4801 gatgtcgccg tgagatcgag cgcgacggcc gaagatctcg tagacgcgtc attcgcggga
     4861 cagcaagaat catatctcaa tgtgacgggg gagtatcaac ttcttgaatc gatcaagaaa
     4921 tgcttttcct cgcttttcac cgatcgagcc atatcctatc gcgtggacaa gggtttcgac
     4981 cagctttcca tcgcgctttc ggtcggcgtg caaaaaatgg tgcgttcgga cgaagcggcc
     5041 agcggcgtga tgttcacgat tgacacggaa tccgggtttt ctgatgcgat catcatcaat
     5101 gggtcatacg gtcttgggga aaatattgtg aagggcaagg tgaatccgga tgagttcatt
     5161 gtccacaagc cgactttgaa gcagggattc aaatcaatcg ttgggaaaaa aatggggagc
     5221 aaagaaacga agctggtcta tagcgtcgga ggaacctcaa ccacccaaaa cgcgattgtt
     5281 tcccaagagg acagaagaag attttgtctc acggacgacg aagccctgga actggcccgt
     5341 tggggacttt tgatcgagga gcactacaaa aaaccgatgg acatcgaatg ggcgaaagac
     5401 ggtcgggacg ggaaattgta tatcgtccag gcgcggccgg aaacagtcca gtcacagaag
     5461 aaaaaaaatg tcctggaaga atatattttg aaaagcaaaa gcaaaatttt gtgcgtcggg
     5521 gcggctgtcg gcggaaagat cggcgcaggg aaggctaatg tcatcaagga tgtcgctcaa
     5581 attgccgatt ttcaaaaagg ggaagtgctg gtgacggaaa tgactgatcc ggattgggag
     5641 ccgatcatga aaatcgcctc ggcgatcgtc acgaacagcg gcgggcggac ttcacacgcg
     5701 gcgatcgtca gccgcgagct cggaattcca tgtattgtcg ggacgggaaa cgcgacagct
     5761 gcgatcaagg gcaaaaaacc ggtgacggtg agttgcgccg aaggggaaga ggggaaagta
     5821 tatgaaggaa ttttgaaatt tgagatcaaa acgacaaatc tcaaaaatct gaaaaaaacg
     5881 aagaccaaga ttatgatgaa cgtcggcaat cccgaccagg ctttcgatct ttctttcatc
     5941 ccaaacgacg gagtggggct ggcgcgggag gaattcatca tcaccgaata cataaagatc
     6001 catccgatgg cgctggttca ttttgaccgg gtgaaggaca aagatgaaaa gaaaaaaatc
     6061 aacgaaatga cccaggggta caagagcaaa gaggatttct ttgtggagaa actggcggaa
     6121 ggaataggac ggatctgcgc cgctttctgg ccgaaagatg tgattgtccg gatgagtgat
     6181 ttcaaaacga atgaatatgc gacattgatc ggcggaaaag tttttgaacc ggcggaggaa
     6241 aatccgatga tcggctggcg gggagcgtcc cgatactatg acgaagatta taaggaagga
     6301 tttcggcttg aatgtctcgc ttttgaaaaa gtccgggagg tgatgggtct cacgaatctc
     6361 aaagcgatga ttccgttttg tcgaactgtc gaagagggca aaaaagtgct ggtcgaaatg
     6421 gaaaaatatg gtcttgagcg gggcaagaat ggtcttcaag tctatgtgat gtgtgaaatt
     6481 ccttcaaacg tcattctcgc caaagaattt tccaaaatat tcgacggatt ttcgatcggc
     6541 tcaaatgatc tcactcaact cacgcttgga gttgaccgcg acagcgcgaa agtggcgcat
     6601 gtctatgacg aacgcaatga agccgtgaaa gaactcatcc ggcaagtgat tgaaaaagcg
     6661 cacaaagccc gccgaaaagt cggcatctgc ggccaggctc cgtcggactt tctcgacttc
     6721 gccaaatttc tcgtcgaaga aaaaatcgat tcgatttctc tcaatcccga cacggtcatc
     6781 aagacgacga tggagattgg gaagatggaa aaataatatt agcaaataat attccgatag
     6841 caaaaatcag gcctaaaagc ttgatttttg ctttataatg ggctattcta catttgttgg
     6901 aagcgtgcgt gagcggctta aacggcagtc ctggaaagac tgtgtgtcga aaggcaccag
     6961 gggttcgaat cctctcgctt ccgcaaaaca aatatccctt tttgtgaggg atatttttgt
     7021 taagcaaatt gactcttatt ttatttcttc tattgcttcc tctaaggctg actgaat
//
LOCUS       DATKGS010000104         9637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_12777, whole genome shotgun sequence.
ACCESSION   DATKGS010000104 DATKGS010000000
VERSION     DATKGS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12777"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2727)
                     /locus_tag="VMQ48_02600"
     CDS             complement(<1..2727)
                     /locus_tag="VMQ48_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HUD08956.1"
                     /translation="MSKQIYNFSLAEILKKFSVSENGLSTEEAKKRLKVYGPNKIEKK
                     NNWRWAKMVGHQFDDALVWILLVAAGLALFFGEMRDVAIIGMIILINATIGFFQEWKA
                     ERILDHIQKLTSDKAFVFRNGKKNEIDSAEIVPGDVIFVSSGDNVPADGYILESYNLH
                     TNSFVFTGESKPEGKMAKTISEEDLSVADVDNMIFSGEQVTMGEARAVVVGTGLNTEL
                     GKIANLATEVKDELTPLQKKIRILGKDVSILAVAIAVAVMIAGSLYHMSLYQNFLFAL
                     AVAVAVVPEGLPAALSVALSLGMRRLLKVNVLAKKLIAVETLGSVNVICTDKTGTITK
                     NELMVTKIILDEKEIVVSGEGYEPKGDFSVGGQKVDPKNIPNLELLLKIATLCNDAAL
                     VYPVRKKETLNNNENPVFSNGVNEKNNFKIAGDPTEGALVVTARKYNPDEKYFERGER
                     KIDENPFESERMRMSVVFENKEENEIFSYVKGSPDVLLGLSPKKLTEEGIVPFSDAEK
                     LKIKKIYDAMSGEALRVLAFVYRPLDNIDKKNYQIESEQDLIWVGMMAMIDPPRKDVA
                     EAIKECRESGIKVIMITGDYEITAAAIARQVGLLNSNVKNNFVINGKELNLLKDGEIL
                     QRIKSGTSVFARITPEQKLRIAGIIKKSGAVIAMTGDGVNDAPALKRADIGVAMGIIG
                     TDVSKEAADMILLDDNFASIVSAIKEGRTIYQNIRKFIYYVFVANAGEFLTAVFGALL
                     FIPAPITAVQILAIDLGVDVLPSFALGLEPPEPGMMKKKPQSADGRLIGTLGFWRLVY
                     VGMIMATGGIIAFLWSMTRGGWHFGQPIDFSSALYIKSTAATYAVLAMAQMANLLQAR
                     SETLSVFAIGFFKNKYIIGAILFSVGLLFAFLYVPFFQKNLHMAPLDLWDWLV"
     gene            complement(2761..3402)
                     /locus_tag="VMQ48_02605"
     CDS             complement(2761..3402)
                     /locus_tag="VMQ48_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HUD08957.1"
                     /translation="MRKKPLVNNEYYHIYNRGVEKRDIFLCDRDYGRFLLSMKEFNGI
                     DPIFSLYWHSQQKRKSDVDVGRLQKLVDIVCYCLNPNHVHFILKQVSEGGISEFMKRL
                     SGGYTKYFNHRYKRSGVLFQGKFKSVHIETNAQLLYLSAYINKNYFIHGYSNNSSWSY
                     SSLLDYTEKRNTEICSPDIILSQFKNKNEYCEFIQENALYMRGKKEDEKYLLE"
     gene            complement(3384..3830)
                     /locus_tag="VMQ48_02610"
     CDS             complement(3384..3830)
                     /locus_tag="VMQ48_02610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUD08958.1"
                     /translation="MLAGKLIDGDLELRFLEKRKVMILKSCAWEFVFEMYLLDTNQKI
                     GAIRLRLKLTPDLEEYGGHVEYEVDKKFRGNNFAARSFTLLLPLFRKIKINPIIITCD
                     PTNIASIKTIEKIGGKLVSAKNIRISSTKKRKTNKYFVYNSCERNR"
     gene            complement(3922..5238)
                     /locus_tag="VMQ48_02615"
     CDS             complement(3922..5238)
                     /locus_tag="VMQ48_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUD08959.1"
                     /translation="MNEQEKFSTENSTENKEEVKKTNKPSYSKVIVVVVVASIVLSSF
                     FGAVFGFMAAKIGQGKFSLSNILKKGQSQTGQSGNSDSGQTINVPSEESAVIQAVKTD
                     SPAVVSIIISKNVPQTPDFFSDPFFDPFGFQQQQPQPQNQNQGNANQKQEIGAGSGFI
                     VTSDGLVVTNKHVISDPSADYTVMTSDQKKYDAKVQAIDPTNDLGILKINATNLPVLE
                     LGNATNLQIGQTVIAIGNSLGEFRNTVSKGVISGLKRNVNAGSDQSGQSELLSQVIQT
                     DAAINPGNSGGPLLDIQGKVIGVNVAMAQGAENIGFALPVDLVKKDLASLKSSGKITQ
                     PYIGVRYVPIDAQIQKDNNLSFDYGALVVRGQQVTDFAVIPGSPADKAGIVENDIILE
                     VNGTKVDADHPLANLLSQYGVGDTLTLKVWHKGNTSDVKVTLEERK"
     gene            complement(5388..5573)
                     /locus_tag="VMQ48_02620"
     CDS             complement(5388..5573)
                     /locus_tag="VMQ48_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HUD08960.1"
                     /translation="MSKPKVNQELCIGCGTCEALCPEMFKVENGKSQVIGESCEKAGC
                     DCNEAVTSCPVSAISME"
     gene            complement(5617..6528)
                     /locus_tag="VMQ48_02625"
     CDS             complement(5617..6528)
                     /locus_tag="VMQ48_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUD08961.1"
                     /translation="MNKKLTEKLAPGHSTCAGCGIPAIARTVLGATEDPVIVVNVTGC
                     LEVTSTLYPYSAWKIPWIHTAFENGGATAAGIDGAIKALRKKNKIPKEKRIQVVVFAG
                     DGGTYDIGLQSLSGALERQHDFLYVCYDNEAYMNTGGQRSGATPYGADTETTPAGKNS
                     FGKTEKRKDLMSIVIAHHIKYAAQANVAYLADLKMKAKKAFALEGPRFLLVLQPCTNL
                     WKYPTSQYVNIGKLATETNFWPLWEYFDGKYKINWATENPKPLEEFLKTQGRFKHLFK
                     PGNEKVIAEMQKEVDVDWKRLVNLADV"
     gene            complement(6529..7674)
                     /locus_tag="VMQ48_02630"
     CDS             complement(6529..7674)
                     /locus_tag="VMQ48_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08962.1"
                     /translation="MSSNKKKLALTGGAAVAEALKQINPDVMPVYPITPQTPIIETFA
                     KMVADGEADTEMIQVESEHSAMSAAVGASAAGARAMTASSSQGILLMAEVLPIASGMR
                     LPILMLVSARAISAPINIHGDHQDVMFVRDSGWIQIFSENSQEVYDNTLIALKLAEIV
                     RLPAMIIMDGFQTSHSVENLEILEDSVAKKFVGEYNPKNYLLNFENPKTFGPVSLPNS
                     YFEFKRQQIEAMDGVIAKLVEVNGNYGEISGRQYDLFEAYKLDDAEKVIVVAGSTAGT
                     AKMAVDELREQGEKVGLLKIKLFRPFPYKQIADALKNAKQIAVLDRTVSFGSVAPLYS
                     EIANSTGRKDLQSYVYGLGGREIFKEGIKKAFQDNWENKEVKYIGLK"
     gene            complement(7736..8002)
                     /locus_tag="VMQ48_02635"
     CDS             complement(7736..8002)
                     /locus_tag="VMQ48_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUD08963.1"
                     /translation="MTNWKDIEEGAIIKHDMEKSPRTGDWRYMKPQVDKKTCIGCGLC
                     FDHCPEACIDYDDERKADIDFDVCKGCGVCATVCPQKAIKMQKN"
     gene            complement(8108..8677)
                     /locus_tag="VMQ48_02640"
     CDS             complement(8108..8677)
                     /locus_tag="VMQ48_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HUD08964.1"
                     /translation="MKIGKKIFEIIFHARGGQGAKTAAELLAQAALLEGKFVQAFPDF
                     GPERTGAPMKTFVRISDEPILTHEPIVDPDAVIVMDETLLGIVEVSKNLDEQESLIVN
                     STKDRETISQKTGYIGNIFPIDATGMSAEIIGEPRPNTAILGKLIKITEVVKLESITE
                     VFREKYLEKIGQEKTEKNIQAIEKAYDSI"
     gene            complement(8771..>9637)
                     /locus_tag="VMQ48_02645"
     CDS             complement(8771..>9637)
                     /locus_tag="VMQ48_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUD08965.1"
                     /translation="PFEGRTWVAYNEPVVTVQVVNDSNCKECDVTDAVKMMKRVMPTL
                     LVKEVQFDSADGKALIDQMKIKSLPAFVFDDSVTKTDIYTQAQDIFQKQGNNYTIDTA
                     QIGIQPGKFLTLPTVNADDAQAGPADAKVKMIIFSDFQCPYCAAFYTQDLKKAMTDYK
                     DKVQFVFKEVPLSIHDKAVPAAMAAECANEQGKFWEMADKLYTDQKTWSAAKTFSATP
                     YASTIAGLNMTQFNQCVSSNKFQDKINADNQSAQDFGISGTPAFFVNDQFFGGVVQYS
                     QLQQAIDAQLAK"
BASE COUNT         2407 a         2445 c         1872 g         2913 t
ORIGIN      
        1 gaccagccag tcccaaaggt caagcggtgc catatgaaga tttttttgga agaaggggac
       61 atacaaaaaa gcaaagagca gtccgactga aaaaaggatt gcgccgatga tatatttgtt
      121 cttgaaaaat ccgatcgcga acacggaaag tgtttccgag cgcgcctgca aaagattggc
      181 catttgcgcc attgcgagaa cggcataagt cgccgccgtg gattttatat agagagccga
      241 actgaaatca attggctgac cgaagtgcca accaccgcgc gtcatcgacc aaagaaaggc
      301 aattatgccg cctgtggcca tgatcatccc cacataaacc agccgccaga atcccaacgt
      361 tccgattagc cgaccgtcag cgctttgtgg cttctttttc atcatccccg gttcgggcgg
      421 ttcaagccca agagcaaatg acggaagaac atccaccccc aagtcaattg ccaaaatctg
      481 cacagctgtg atgggcgccg ggatgaaaag caaggctccg aagacggccg tcaaaaattc
      541 tccagcgttg gccacaaaaa cataatagat aaatttccgt atgttttgat atatggtccg
      601 gccttcttta atagcgctca caatcgacgc aaaattgtcg tcaagcagga tcatatccgc
      661 cgcttctttg gaaacatccg tcccgatgat ccccatcgcg acgccgatgt ccgcacgctt
      721 gagagccggc gcgtcgttca ccccgtcgcc cgtcatcgca atcacggcgc cggatttttt
      781 gatgattccc gcaatccgca atttctgttc gggcgtaatt cttgcaaaga cagacgtgcc
      841 actctttatt ctttgtaata tctctccatc tttcaataag ttcaattcct tgccatttat
      901 aacaaaatta tttttgacat tggaatttag taaaccgact tgccttgcta ttgccgctgc
      961 agttatctca tagtctcccg taatcatgat caccttgatc cccgattcgc gacattcttt
     1021 gatcgcttcc gccacgtctt ttcggggcgg gtcgatcatc gccatcatcc cgacccagat
     1081 caaatcctgc tcggattcaa tttgataatt ttttttatca atattatcaa gcggccgata
     1141 gacgaaagcc agcactcgca gcgcctctcc cgacatcgca tcgtatattt tttttatttt
     1201 taatttttcc gcgtcggaaa aggggactat cccctcttca gtcaactttt tgggggagag
     1261 gccgagcaaa acatccggcg agccttttac ataggaaaaa atctcgtttt cctctttgtt
     1321 ttcaaaaaca acactcatgc gcatccgttc cgactcaaac ggattttcat caatcttcct
     1381 ctcccctctt tcgaaatatt tttcatccgg attatatttt ctggccgtaa cgaccagcgc
     1441 tccttccgtc ggatcgcctg caattttgaa attatttttt tcgttcaccc cgttagaaaa
     1501 tacgggattt tcattattat ttaaagtctc tttctttcta acggggtaaa ccaacgccgc
     1561 gtcattgcaa agagtggcga ttttcagcaa taattcgaga tttggaatat ttttcggatc
     1621 gactttttgt ccgccgacag aaaaatctcc ttttggctca tatccttccc cgctaactac
     1681 tatttctttt tcatccagga tgatttttgt caccatcaat tcatttttcg tgatcgtccc
     1741 ggttttgtcc gtacagatca cgttcacgga cccaagcgtt tcgaccgcga tcaatttttt
     1801 tgcaaggaca ttcaccttga gcaaccgccg catcccaagc gaaagagcga ctgaaagcgc
     1861 cgctgggagt ccctcgggca caaccgcaac cgctaccgcg agagcgaaaa ggaaattttg
     1921 atagagcgac atatgataaa gacttccggc aatcatcacc gcaaccgcga tcgccaccgc
     1981 caaaatcgac acatcctttc ccaatattct tatttttttc tgaagcggcg taagttcatc
     2041 cttcacttcc gtcgcgagat tcgcaatctt tccgagctcc gtgttcagtc ccgtcccgac
     2101 cacaaccgcc cttgcctccc ccattgtcac ctgctcgccc gaaaaaatca tattgtccac
     2161 atcggcaacc gacaaatctt cctcgctgat tgtcttcgcc attttccctt ccggttttga
     2221 ttctcccgtg aaaacaaaac tgttcgtgtg aagattgtag ctttccaaaa tatatccgtc
     2281 tgccggaaca ttgtccccac tggaaacaaa aatcacatcg cccggcacaa tttcggccga
     2341 atcaatttca ttttttttcc catttcgaaa aacaaaagct ttgtctgaag tgagtttttg
     2401 gatatggtcc aaaatccgct ccgctttcca ttcctgaaaa aatccgatgg tcgcattgat
     2461 gagaatgatc attccaatga tcgccacatc ccgcatttcc ccgaaaaaaa gtgccagccc
     2521 cgccgcgact aagagaatcc acaccagcgc gtcatcgaac tgatgtccga ccattttcgc
     2581 ccatcgccag ttgttctttt tttcaatttt gttcggcccg taaactttga gtctcttttt
     2641 cgcttcttca gtcgaaagtc cgttttcaga caccgaaaac tttttcagaa tttctgcgag
     2701 agagaaattg tatatttgtt tggacataat attttgtaga cgtccgacgt ctacaagtaa
     2761 ttattctagc aagtatttct catcttcttt ctttcctctc atatacaggg cattttcctg
     2821 tataaactca cagtactcat tcttattttt aaattgacta aggataatat ctggactgca
     2881 gatttccgta ttcctttttt cagtataatc caaaaggctt gaatatgacc aactggaatt
     2941 attgctgtat ccgtgaatga aataattttt gttgatatag gctgataaat agagaagttg
     3001 tgcgtttgtt tcaatgtgaa ccgatttaaa ctttccctga aatagaacac cggatctttt
     3061 atagcgatgg ttgaaatatt tggtatagcc accactcaat cttttcataa attcagaaat
     3121 gccgccttcc gagacttgtt tcaaaataaa atgaacatga ttcggattga ggcaatagca
     3181 aactatgtcc acaagctttt gtagacgtcc aacgtctaca tcgcttttcc tcttttgctg
     3241 actatgccaa tacaagctaa aaatagggtc aatcccatta aactctttca tagaaagcag
     3301 gaatcttcca taatccctat cacacaaaaa aatatcccgt ttttctactc ctcggttata
     3361 gatatgatag tattcattat ttactagcgg tttctttcgc atgaattata tacgaaatat
     3421 ttgttagttt ttcgtttttt agtagatgat attcgaatat ttttcgccga aaccagctta
     3481 cctccaattt tctcaattgt ttttattgag gcgatattag ttggatcgca agttataata
     3541 attggattaa ttttaatttt tctaaacaag gggagtaaca gagtaaaact ccttgcggca
     3601 aaattatttc cgcgaaattt tttatctact tcatattcca catgtccacc atattcttcc
     3661 aaatctggag tcagtttcag cctcaatcga attgcgccaa tcttctgatt cgtatccaac
     3721 aagtacattt caaaaacaaa ctcccaagcg caacttttca aaatcattac ttttcgtttc
     3781 tcaaggaatc ttaattctaa atcgccgtcg attaattttc ctgcaagcat agctccatta
     3841 tatattcaac acaaaaataa aagaagcggc tttgtttccg cttccttttt gcaatcttgt
     3901 agacgtcgga cgtctacaaa tctatttcct ttcctcaagc gtcactttga catcgcttgt
     3961 gtttccttta tgccaaactt tgagcgtgag agtatcgccg acgccatatt ggctcaaaag
     4021 attcgcaagg ggatggtcgg cgtcgacttt cgtgccgttc acctcaagga taatgtcgtt
     4081 ttctacgatt cccgctttgt cggccgggct tccgggaatg accgcgaaat ccgtcacttg
     4141 ctgaccacgg acaaccagag cgccataatc gaaggataaa ttgttgtctt tctggatttg
     4201 cgcatcaatg ggaacatatc gcacgccaat atacggctga gtgattttcc cgctgctttt
     4261 caagctggcc aaatcttttt tcaccagatc aaccggaagg gcaaaaccga tattttccgc
     4321 gccttgcgcc atcgcgacat tgactccgat tacttttcct tgaatatcca agagcgggcc
     4381 accagaattg cccggattga tcgccgcatc agtttggatc acctggctca aaagttcgga
     4441 ttggccgctt tggtcagaac cagcattgac atttcgcttg agtcctgaga tcacgccttt
     4501 gctcaccgtg tttcggaatt caccgagtga atttccgatc gcgatcaccg tctggccgat
     4561 ctgcaaatta gtggcgtttc caagctctaa aaccggaaga ttggtcgcgt ttattttcaa
     4621 aattccaaga tcgttcgtcg gatcgatggc ctgcactttc gcatcatatt ttttttggtc
     4681 gcttgtcata acagtatagt cagcgctcgg atcggaaata acgtgcttgt tcgtcaccac
     4741 aagcccgtcg cttgtcacaa tgaatccaga accggctcca atctcctgct tttgatttgc
     4801 gtttccctga ttttggtttt ggggttgcgg ctgttgttgc tgaaaaccaa agggatcaaa
     4861 gaaaggatcg gagaaaaaat ccggcgtttg cgggacattt ttggaaatga taatgctcac
     4921 tactgcggga gaatccgttt tcacggcttg gatgacagct gattcctcac tgggaacatt
     4981 gatcgtttgg cctgagtcgg aattgcccga ctgtcctgtc tggctttggc cttttttcaa
     5041 aatattcgac agactgaatt ttccctgccc tattttcgcc gccatgaaac caaaaaccgc
     5101 cccgaaaaaa gaggaaagga caatcgaagc aacgacgaca accacgatga ctttgctgta
     5161 gcttggtttg tttgtctttt tcacttcttc tttattttca gtagaatttt cagtagaaaa
     5221 tttttcttgt tcgttcatta aggtaaaata ttatttgcta aacctcctcc cctgccaagg
     5281 ggaggtgccc gaagggcgga ggggtttgga taacgcacag aaataaaacc acccctcgat
     5341 cccctccttg aaaaaggagg ggaggattct aaaaaagaaa tttcattcta ttccattgaa
     5401 atcgcgctca ccggacagct tgtcacggct tcattgcaat cacagccggc cttttcgcaa
     5461 ctttcaccga tcacctgcga ttttccgttt tcaactttga acatctcggg gcaaagcgcc
     5521 tcgcaagtcc cgcatccgat gcaaagttcc tggtttactt tgggttttga catatgtttc
     5581 gcttaattat tacgatatgt tttgcttatt ctttcattat acatccgcca aattcaccaa
     5641 tcttttccaa tccacatcca cttctttttg catttcggcg atgacttttt cattccccgg
     5701 cttgaaaaga tgcttgaatc tcccttgcgt tttcaagaat tcttcgagcg gtttcggatt
     5761 ttccgtcgcc cagtttatct tatatttccc gtcaaaatat tcccaaagcg gccagaaatt
     5821 ggtttctgtt gccagttttc cgatgtttac gtattgagaa gtcggatatt tccacaaatt
     5881 cgtgcaaggc tgaaggacga gcaaaaatcg cggaccttca agcgcaaagg ctttcttagc
     5941 tttcatcttg agatcagcca gatacgcaac atttgcctgc gccgcatatt tgatgtgatg
     6001 cgcgatcacg atcgacatca gatcttttct tttttccgtt ttgccaaaac tgtttttccc
     6061 ggccggggtc gtttctgtgt ccgcgccata gggagtcgct ccgcttcttt gcccgcccgt
     6121 gttcatatag gcttcgttgt catagcagac atagagaaaa tcatgctgac gctcaagcgc
     6181 accggacaga ctctgcagac cgatgtcata cgtcccccca tcacccgcga aaacgacgac
     6241 ttgaattctt ttttcttttg gaattttgtt ttttttgcgc aaagccttga tcgcgccatc
     6301 gattccggcc gccgttgctc cgccattttc aaatgccgtg tggatccagg gaattttcca
     6361 cgccgaatag ggatagagcg ttgacgtcac ttcgagacat cctgtgacat tgacgacaat
     6421 caccggatct tccgtcgcac cgagaaccgt ccgcgcaatc gccggaatcc cgcaacccgc
     6481 gcaggtggaa tgtccgggag ccaatttttc tgtgagtttt ttgttcattt atttcaagcc
     6541 aatatatttt acttctttgt tttcccaatt gtcctgaaat gcttttttga taccttcttt
     6601 gaaaatttcc cggccgccta gaccataaac atatgattgt aaatcttttc gtcctgttga
     6661 attcgcaatt tcggaatata gcggcgcgac gctcccaaac gaaaccgtcc ggtctaaaac
     6721 ggcaatttgt ttcgcatttt tcagtgcatc agcgatttgc ttgtacggaa acggacgaaa
     6781 taattttatc ttgagcagcc caactttttc accttgctct cgcaattcat ctactgccat
     6841 tttggctgtc ccagccgtcg atccggccac gacgatcact ttttctgcgt catctaattt
     6901 atacgcttca aataaatcat actgccgccc gctaatttct ccataatttc cattaacttc
     6961 aaccaatttc gcaattaccc catccatcgc ctcaatttgt tgccttttga attcgaaata
     7021 tgaattggga agcgaaaccg gcccgaaagt ttttggattt tcaaagttta atagataatt
     7081 tttgggatta tattctccga caaatttttt agcgactgaa tcttccaaaa tttctagatt
     7141 ttcgacgctg tgtgaagttt gaaaaccgtc catgatgatc atggcaggca acctgacgat
     7201 ttcagccagt ttgagtgcaa ttagagtatt atcatagact tcttgggaat tttccgaaaa
     7261 tatctggatc cagccggaat cccgcacaaa catcacatcc tgatggtcgc cgtggatatt
     7321 gatcggagcc gagatcgccc gcgcgctcac cagcatcaaa atcggcaggc gcattcccga
     7381 cgcgatcggc aaaacttccg ccatcagcaa aattccctgt gagcttgaag ccgtcatcgc
     7441 ccgcgctccc gcggcgctcg cgcctaccgc cgcactcatc gcactgtgct ccgattcgac
     7501 ctggatcatc tccgtgtccg cttcaccgtc cgcgaccatt ttggcaaaag tttcaatgat
     7561 cggcgtctga ggcgtgatcg gatacaccgg catcacatcc ggattgatct gtttcaaggc
     7621 ttccgccaca gcagcacccc ccgtcaaagc taattttttt ttattagaac tcattttatt
     7681 ctactctacc aactcttcta tacttagaag tatggaagaa agtgtgtatt aaatctcaat
     7741 ttttctgcat ttttatcgcc ttttgagggc aaaccgtcgc gcagacgccg caacctttgc
     7801 aaacgtcaaa atcgatgtcc gctttacgct cgtcatcgta gtcaatacaa gcttccgggc
     7861 aatggtcaaa acacaacccg cagcctatgc aggttttttt gtccacttgc ggtttcatat
     7921 accgccaatc acccgtgcgc ggagatttct ccatatcatg ttttataata gctccctctt
     7981 ctatatcttt ccaatttgtc attttattat aatttccaat tatcaatttt caatttacaa
     8041 taaattttca atgattcaat ttccaatgtt tgaaaattta aaatttagga attgaaaatt
     8101 gaaaatttta gatactatca taggcttttt caatcgcctg aatatttttt tccgttttct
     8161 cttgcccgat tttttccaga tatttttctc gaaatacttc tgtgatgctt tccagtttca
     8221 caacttcggt gattttgatc aattttccca aaatcgccgt attcggtcgt ggttcgccga
     8281 tgatttcggc ggacatcccg gtcgcgtcaa tcgggaatat atttccaata tatccggttt
     8341 tttgcgaaat agtttcccga tcttttgtcg aattgacaat aagactttcc tgctcatcaa
     8401 gatttttcga aacttcgacg attcccaaaa gcgtttcatc catcacaatc accgcgtccg
     8461 gatcaacgat cggctcgtgg gtcaagatcg gctcgtcgga aatccgcaca aacgttttca
     8521 tcggcgcccc cgtccgttcc ggaccaaaat cgggaaaagc ctgaacgaat ttcccttcga
     8581 gtaaagccgc ctgcgccaaa agttcggcgg ctgtcttggc tccctgcccg ccgcgagcgt
     8641 gaaaaataat ttcgaaaatt ttctttccga ttttcatata ctaattatac cacaaacaaa
     8701 aaaatacaaa aagaaatcga gcctttcgac ccgatttcta tactaaatac tagatactag
     8761 atacttaatg ctacttcgcc aattgcgcgt cgatagcctg ctgcaactgg ctatattgca
     8821 caactccgcc gaagaactgg tcgttcacga agaaagccgg tgtaccgctg attccaaaat
     8881 cttgcgctga ttgattgtca gcgttgatct tgtcttggaa tttattggac gacacacact
     8941 ggttgaattg agtcatattg agaccagcga ttgttgacgc gtacggcgtc gcgctaaagg
     9001 ttttggccgc gctccaggtt ttttggtccg tatagagctt gtccgccatt tcccagaatt
     9061 ttccctgctc attggcacat tcagccgcca tagccgccgg caccgctttg tcatggatag
     9121 aaagcggaac ttccttgaaa acaaattgga ctttgtcttt gtaatcagtc atcgccttct
     9181 tgagatcctg ggtatagaag gcggcgcaat atggacattg gaaatcgctg aagatgatca
     9241 ttttcacttt ggcgtccgcc ggtcccgctt gcgcgtcatc cgcattgact gtcggcagcg
     9301 tcaagaattt tcccggctga atgccgattt gagccgtatc gatggtataa ttgtttccct
     9361 gcttttgaaa aatatcctgc gcctgggtat atatgtccgt ttttgtcacg ctgtcatcga
     9421 aaacaaaggc gggaaggctc ttgatcttca tttggtcgat caaagctttt ccatccgcgc
     9481 tgtcaaactg cacttccttc acaagcagcg tcggcatcac tctcttcatc attttgaccg
     9541 cgtccgtgac gtcacattct ttgcaattgc tgtcattcac cacctggaca gtgacaaccg
     9601 gctcgttgta agcgacccat gtccgtcctt cgaatgg
//
LOCUS       DATKGS010000105         4821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_155671, whole genome shotgun sequence.
ACCESSION   DATKGS010000105 DATKGS010000000
VERSION     DATKGS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155671"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VMQ48_02650"
     CDS             <1..271
                     /locus_tag="VMQ48_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08966.1"
                     /translation="GSARKRAIEKMIILKDREPVHSHINKERLWVRKMYWPKMGLLTH
                     HDPLSKKKILKDKRIKNGDNISKLLENKDLSYYNNGITRGQYSTN"
     gene            287..1060
                     /locus_tag="VMQ48_02655"
     CDS             287..1060
                     /locus_tag="VMQ48_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08967.1"
                     /translation="MKRFFLYFLLILFIVGLAMLAYGFWNAKNIRLWAAAAGQIKSQH
                     DIGSKEKDITNRFEASGGKKMEDLKNELAGFSSDLDSIQKETESAKQETEKLGAPRAA
                     KPTQQELVDFYDQSGQQIQNLESVSTFMSQLFEVAVIFDGIKQDTKLEDIQAMIAEAK
                     AKSGGISAGILPDVVQASGNDLKTATDNFLDTIDQTAAGKVEGSDQLNTAYADFSQKE
                     NDFFSAAKKYISSFQNTDLVGQKIDTDLLILRKVKFSIK"
     gene            1086..2408
                     /locus_tag="VMQ48_02660"
     CDS             1086..2408
                     /locus_tag="VMQ48_02660"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUD08968.1"
                     /translation="MPKRKTIIWIVIGAAVLIGIVYFAFLRKPKVQYNTVEVKKGELT
                     ETVSVTGTLKANDDIGLNFETPGRIKESRIRVGDKVAKGDVLAILDQTNLQNDLDQAQ
                     ANLDKARADAGANSDIVHTDDVEVENAKSSLEDTKSLNDANVKAADQTYTDAKNKLDD
                     AQSYYDQVKSESGASSSTTKLAKLTLDAAQASYNSAKKDRDVVDQQADLAKTGAENNL
                     NSAEANLSSAKSKYVSAGNNATVKSFAAAYETALNNLDKAVLRAPTNGVIKKVNFKPG
                     EVIGSPSVTSQSGFFAEMISFDNVFEAQVSEADIAKVAVGQSTKLTFDALPSESFDGE
                     VISIEPSATIVQDVVDFVVKISLSKSDLRLKDGMSADVDISINKKEDVISIPQRAVQD
                     SSGKKIVQVLENGKSVDREVKLGLEGDGGLVEIVSGLSEGDQVITSIK"
     gene            2568..3293
                     /locus_tag="VMQ48_02665"
     CDS             2568..3293
                     /locus_tag="VMQ48_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUD08969.1"
                     /translation="MLISVKKLCKEYDNDGVVTQALCGVSFDIKEGEFVAIMGPSGSG
                     KSTLMQILGFLDRPTNGEYLFEGKNTKNFSDDDLAYLRNQRIGFVFQSFNLLPRTTVF
                     ENIELPLIYDERKESQSLIRQKVEQALKAVNLEERINNFSNQLSGGEQQRVAIARALV
                     NDPALILADEPTGNLDSKNGLQIMKILQKLNDDGHTIVMVTHETYTSEHAKRIIEMLD
                     GNVVKDKLVAKRRIADGEERLLK"
     gene            3425..4669
                     /locus_tag="VMQ48_02670"
     CDS             3425..4669
                     /locus_tag="VMQ48_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUD08970.1"
                     /translation="MRFSDPLKISYRNLTAAKFRSFLTILGIVIGIASVIIIMAIGTS
                     AQELILDQVRGIGSNLIGVLPGASEEKGPPASALGVVLTTLKYDDFKALLQKNNVPNA
                     IDGAAYLSGNNTVQFGSINRNYTYQGTTAGYVGTENAEIDKGRFFAKEEETSLSRFAV
                     LGSSVADDLFGKNIDPIGKKIKIGDQSFEVIGVFKKRGSTAFSNQDTTIYMPIFTAQK
                     IMLGVDYLNFVRIKVDSEANIDRAKADITATLRERHHIKKASNDDFSVRDQQAGLDVI
                     TNVTGAIKYFLTAIAAISLLVGGVGIMNTMLISVNQRIREVGLRKAIGAKNSAIMSQF
                     LVESVVVTLIGGIVGILIGVFVSFVAALVIQKLGYNWPFVVTFSSIALAVGISFLVGI
                     FFGIYPARKAAKLNLVEALRYE"
BASE COUNT         1501 a          902 c         1176 g         1242 t
ORIGIN      
        1 gggttcggct aggaaaagag caattgaaaa aatgatcata ctaaaagata gagaaccggt
       61 gcacagccat atcaataaag agaggctatg ggttagaaaa atgtattggc caaaaatggg
      121 actcttaacg catcatgacc cgttgtctaa gaaaaaaata ttaaaggata aaagaattaa
      181 aaacggggac aatatcagta aattgctaga aaataaagat ttatcatatt ataataatgg
      241 gatcactagg ggccagtaca gcacaaatta gaaattaaaa aaaacaatga aaagattttt
      301 tctctatttt ctcttgatat tgttcattgt cggccttgcg atgctggcct acggtttttg
      361 gaatgcgaaa aatatccgtc tctgggcggc cgcagccggg caaatcaagt cgcagcacga
      421 tatcggcagc aaggaaaaag atatcacaaa ccggtttgaa gccagcggcg gaaagaaaat
      481 ggaagatttg aagaatgaat tggccggttt ctcatcggat ctcgactcaa ttcaaaaaga
      541 aaccgaatcg gccaagcagg aaacggagaa acttggcgcg ccgagagcgg ccaagccgac
      601 gcagcaagaa ctcgtcgatt tttatgacca gagcggacag caaattcaaa atcttgagag
      661 cgtctccaca ttcatgagcc agctcttcga agtcgcggta atctttgacg gcataaaaca
      721 ggatacaaag cttgaggata tacaggcgat gatcgccgaa gcaaaagcga aaagcggtgg
      781 gatcagtgcg ggtattttgc cggacgttgt gcaggcttca ggcaatgatt tgaaaacggc
      841 gacagacaat tttttggata cgattgacca gaccgctgcg ggaaaagttg aaggcagcga
      901 ccagctcaac acggcctatg ccgatttctc gcagaaagaa aatgattttt tttccgctgc
      961 gaaaaagtat ataagttctt ttcaaaatac ggatctggtc ggacaaaaaa tagataccga
     1021 tcttttaatt ttgagaaaag tgaaatttag tataaaatga aaacagaaag gtctcttctc
     1081 aaagtatgcc caaaagaaaa actataattt ggatagtcat cggagcggct gtgctgatcg
     1141 gcatagtgta ttttgcgttt ctgcgaaagc caaaagtcca atataatacc gtcgaggtga
     1201 aaaagggaga attgacggag acggtgtcag tgaccggaac attgaaagcg aatgatgata
     1261 tcgggctaaa ctttgaaacg cccggccgga tcaaagaatc cagaataaga gttggcgaca
     1321 aagttgccaa gggcgatgtt ttggcaattc ttgaccaaac caatttgcaa aatgacttgg
     1381 atcaggctca ggccaatctt gacaaggctc gcgcagacgc gggagcaaat tcagatattg
     1441 tgcatacgga tgatgtggaa gtggaaaacg cgaaaagttc tcttgaagat acaaaaagtc
     1501 tcaatgacgc caatgtcaaa gcagctgatc aaacctatac tgacgcgaaa aataagctgg
     1561 acgatgctca aagctattat gatcaggtga aaagtgaaag cggtgcgtcg agttcgacca
     1621 caaaattggc aaaattgact ttggatgcgg cccaggcaag ttataacagc gcgaaaaagg
     1681 atcgcgacgt cgttgatcag caagcagatc ttgcaaaaac aggagcagag aacaacttga
     1741 attccgccga ggcgaatctc tcgtctgcca aatcaaagta tgtgtcagcg ggaaacaatg
     1801 cgaccgtgaa aagttttgcg gcggcctatg agacggctct caacaatttg gacaaagcgg
     1861 ttcttcgcgc gccgacaaat ggcgtgatca agaaggtgaa tttcaagccc ggcgaggtca
     1921 ttggttcgcc aagcgtcact tcgcagagcg gattcttcgc ggaaatgatt tcctttgaca
     1981 atgtttttga ggcgcaggtt tcggaagcgg acattgccaa ggtggcagtg ggacaatcga
     2041 ccaagctcac ttttgacgct cttccgagtg aatcatttga tggagaagta atttcgatcg
     2101 agccttccgc taccattgtt caagacgtgg ttgattttgt ggtcaagata tccctttcaa
     2161 aaagtgattt gcgactcaaa gacgggatga gcgccgatgt ggatatatcg atcaataaaa
     2221 aagaggatgt catatcaatt ccccaaaggg cagtgcaaga ttcaagcgga aaaaaaatcg
     2281 tccaagttct tgaaaatgga aaatcggtgg atcgcgaggt gaaattgggg cttgaaggcg
     2341 atgggggatt ggttgagata gtgagcggat tgagtgaagg agatcaagtt ataacatcga
     2401 taaaataatt caagtatttc tgtccaataa ttttcaattt tcaatttacg aattttcaaa
     2461 caattttcaa tgcttcaatg aatcaatttc aaaatttttt catttagaca ttgagaaatt
     2521 ctggcattgt ttggaaattg aaaattagaa attggaaatt ttttattatg cttatcagcg
     2581 taaaaaaatt gtgtaaagaa tatgataatg atggcgtggt cacgcaggca ttgtgcgggg
     2641 tttcttttga tataaaggaa ggagaatttg tggcgatcat ggggccgagc gggagtggaa
     2701 aatcaacact gatgcagatt ctcggttttc ttgaccgtcc gacaaatgga gagtatctct
     2761 ttgaagggaa aaacacgaaa aatttttccg atgacgattt ggcctatctt cgcaatcaaa
     2821 gaatcggttt tgtgttccag tctttcaatt tgttgccgcg aacgacggtt tttgagaata
     2881 ttgagttgcc cttgatatat gacgagcgca aagaatcaca gagtctcatt agacaaaaag
     2941 tggagcaggc tctcaaggcg gtcaatcttg aagaaaggat caacaatttt tccaaccaac
     3001 tttcgggcgg tgagcagcaa agagtcgcca ttgcgagagc cttggtcaat gatcccgccc
     3061 tcattttggc cgacgaacca acaggaaact tggattccaa aaatggtttg caaataatga
     3121 aaattctcca aaaactgaat gacgatgggc atacgatcgt gatggtgacg cacgaaacat
     3181 atacctcgga gcatgcgaag cgtatcattg aaatgctcga cggaaatgtc gtgaaggaca
     3241 aattggtagc gaaaagaaga atcgcggatg gggaagagag actcttgaaa taatttcaga
     3301 attacttaat tttcaatttt caaacaatga tttaatttct caatttttca atgatttatt
     3361 ttagaattta gtcattgaaa attgtttgaa aattttgaaa ttgattaatt gaaaattaaa
     3421 gcgtatgcgt ttttctgatc cgctcaaaat ttcatatcgg aacctcacgg cggccaaatt
     3481 tcggtcgttt cttactattc tcggaattgt cattggcatt gcttcagtga tcatcatcat
     3541 ggccatcgga acgagtgccc aggaactgat tcttgaccag gttcgcggga tcggatcaaa
     3601 cctcatcggt gttttgccgg gagcatccga ggaaaaaggg ccgccggcaa gcgccttggg
     3661 agtggttctc acgacactca aatatgatga tttcaaagct ttgcttcaaa aaaacaatgt
     3721 gccaaacgcg attgacgggg cggcctattt gagtggaaac aatactgttc aattcggaag
     3781 catcaacagg aactatacat atcaaggtac tacagccggc tatgtcggga cggaaaatgc
     3841 tgaaatcgac aagggccgtt tttttgcgaa agaagaagaa acaagcttgt ctcggtttgc
     3901 tgttttgggt tcgagcgtcg cagatgatct ttttggaaaa aatatagacc cgatcggaaa
     3961 aaaaataaaa atcggtgatc aaagttttga ggtgataggt gttttcaaaa aaaggggatc
     4021 gacggccttt tccaatcagg atacgaccat atatatgccg attttcacgg cgcaaaagat
     4081 aatgcttgga gttgattatc tcaattttgt ccgtatcaaa gtggatagcg aagcgaatat
     4141 tgaccgggca aaagcggaca tcacggcgac gctgcgcgaa agacatcaca tcaaaaaagc
     4201 gtcgaatgac gatttttctg tgcgtgatca gcaggcggga cttgacgtga tcacgaatgt
     4261 cacgggcgcc atcaaatatt tcttgacggc gatcgcggca atctcccttt tagtgggcgg
     4321 agttgggatc atgaatacaa tgcttatcag cgtcaatcag cgtatccgcg aagtgggact
     4381 gcgcaaggcg atcggagcca aaaattccgc cataatgtcc cagtttctcg tggaatcagt
     4441 cgtagtgacg cttatcggag gtattgtcgg aatattgatc ggtgtttttg tctcttttgt
     4501 ggcggcattg gtcatccaaa aattgggata caattggcct tttgtggtga cattttcttc
     4561 aattgctctg gctgtcggca tttctttttt ggtgggaata tttttcggca tctatccggc
     4621 tcgcaaagcg gccaagctca acttggtgga agcgctgcgc tatgaatagc gcaaaatttt
     4681 gaatttccgg ttttgaaatt gttatggcaa atattttcaa aaaatactta tattccagca
     4741 tcaaatcccc tcatgaatgg gccgttgttt ttcgtcttgc ttttcggaac ctcaaaacaa
     4801 acaagatgcg gacagtcctc a
//
LOCUS       DATKGS010000106         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_98483, whole genome shotgun sequence.
ACCESSION   DATKGS010000106 DATKGS010000000
VERSION     DATKGS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98483"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..687
                     /locus_tag="VMQ48_02675"
     CDS             178..687
                     /locus_tag="VMQ48_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HUD08971.1"
                     /translation="MKNIELKIKIDDFKSILAGLKKSRAIKRGVLKQTDTYFNCEGKR
                     LKLREENNRKFQLVLYVRPDKKEAKVSDFETIDFDKQQAQALKAVLKKAYGEKVVVKK
                     ERKLWMYKNTRIHLDKVEKLGTFLELETLVTKDLALAKKEYDKVVDLLDLDRYKKLER
                     SYSDMLLAK"
     gene            725..1399
                     /gene="radC"
                     /locus_tag="VMQ48_02680"
     CDS             725..1399
                     /gene="radC"
                     /locus_tag="VMQ48_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HUD08972.1"
                     /translation="MPKIKDIPKIERPREKIEKYGPGKLSNAELLAILLRTGTKGINV
                     VELSKRILTKFPKNTLSKAGFKELKNTFGLGKAKAAEIMACFELGRRLLKDKPHKLIL
                     SPQDVHREMKNYRNHKQEHFVVFFLDSRSGEIASQVVSVGTLNASLVHPREVFEPAIN
                     CSAAQIIVAHNHPSGDAEPSEDDLAVTRRLVEAGKLLGIEVIDHVIVAKDDFASLKER
                     NLAGLT"
     gene            1447..1812
                     /locus_tag="VMQ48_02685"
     CDS             1447..1812
                     /locus_tag="VMQ48_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HUD08973.1"
                     /translation="MRSKTTIPITEARRKIFDIAEEVQKPGVYYFLTDKGRPKAVIMS
                     IDEFESWVETLEVMQDFPDLKKDIEEARKAFKTGEYKKWFSLDQLLAREGFILADNPK
                     KKYAAIRPKSKTKRAKKTR"
     gene            1760..2017
                     /locus_tag="VMQ48_02690"
     CDS             1760..2017
                     /locus_tag="VMQ48_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUD08974.1"
                     /translation="MQQYDLKVKPNAQKKLDKLPKADYHRVLAAFSILSHNPFIGKKL
                     KGEYAGSYSYRVWPYRIIYDIYKKELLVLVVRVGHRQRVYR"
BASE COUNT          688 a          429 c          521 g          465 t
ORIGIN      
        1 cgatcaatac gaaaaaataa aagagttgag ttaaaataaa aaaaggagga agccagcgtg
       61 accgctgaaa agatcgaata ggagggtcga gggctctcag atatcatcaa ggcgcacaat
      121 atcgcgataa ggcctttttt acttgaaaaa taaagggatt tggagtaggg tgaggatatg
      181 aaaaacatcg agctcaagat caaaattgac gatttcaagt ccatcctagc cggactcaaa
      241 aagagccggg ccatcaaaag gggcgttttg aaacaaactg acacctattt caattgcgaa
      301 gggaaaagac tgaaacttcg ggaagaaaac aataggaagt tccagctggt gctatatgtt
      361 aggccggaca aaaaagaagc caaggtgtct gatttcgaga caatcgattt tgacaagcag
      421 caggcgcagg cgctgaaagc ggttttgaaa aaagcgtacg gagaaaaagt ggtcgtgaaa
      481 aaagaacgca aactttggat gtacaagaat accaggattc atctcgataa agttgaaaaa
      541 ctgggaacct ttttggagct ggagacgcta gtcacaaagg atctggcttt ggcaaagaaa
      601 gaatacgaca aagtcgtgga tcttttggac ttggatagat ataaaaagct ggaaagatcc
      661 tacagcgata tgttgctggc aaaatagtaa atcaatgatg gagtatagga ataaataaaa
      721 atcaatgccg aagataaaag acattccgaa aatcgaaaga ccgcgggaaa aaatcgaaaa
      781 atacggtccg ggaaagcttt caaatgcgga gcttttggcg atacttttga gaaccggaac
      841 caagggcatc aatgtcgtcg aactgtccaa aaggatcctc accaagtttc ctaaaaatac
      901 attgtctaag gccggcttca aagaattgaa aaatacgttt ggccttggaa aagcgaaggc
      961 agcggagatc atggcttgct ttgaactcgg ccggcggctt ctgaaagaca agccgcacaa
     1021 attgattctc agtccgcaag acgtacatcg cgagatgaaa aattatcgaa accacaagca
     1081 ggaacatttt gtggtttttt tcttggactc gcggagcggt gagatcgcca gccaggtcgt
     1141 ttccgtcggc accttgaatg cgtccctcgt ccaccccaga gaagtcttcg agccggcgat
     1201 caattgcagc gccgcgcaga tcatcgtggc tcacaaccat ccctcgggag acgctgagcc
     1261 gtcggaagat gatcttgcgg tcactcggcg cttggttgaa gcaggcaagc tcctggggat
     1321 cgaggtaatc gaccacgtca ttgtcgccaa agacgacttc gcgagcctca aagaaaggaa
     1381 tttggccggc ttgacgtaat acgaggtgta cactattatg tacatataac aagtagcagc
     1441 aaatatatgc gctccaaaac aacgattccc atcacggaag cccgccggaa aattttcgat
     1501 atagccgaag aggttcaaaa gccgggcgtc tattattttt tgacagataa aggtcgtccc
     1561 aaggcggtga tcatgtctat cgatgagttt gaatcctggg ttgagacttt ggaggtaatg
     1621 caggatttcc cggatctcaa aaaagatata gaggaagcga ggaaggcatt taaaaccgga
     1681 gaatacaaaa agtggttttc tctcgaccag ctgttggcgc gagagggctt tatattagct
     1741 gataatccaa aaaagaaata tgcagcaata cgacctaaaa gtaaaaccaa acgcgcaaaa
     1801 aaaactcgat aagctcccga aagccgacta tcatcgggtg ctggcggctt tttcgatatt
     1861 gtcccataat cctttcattg gaaaaaaatt gaaaggcgaa tacgccgggt cgtattccta
     1921 tcgcgtctgg ccgtatagga taatttacga catatacaaa aaagaattgc tcgtgcttgt
     1981 cgtccgcgtg ggccatcggc aacgggtgta tcgataaaat attatgtttg aacaatcgct
     2041 aaaaaatatc gacgacattc ttcgggctga caatgccgga agcgctcttg actatcaccc
     2101 ccc
//
LOCUS       DATKGS010000107         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_63626, whole genome shotgun sequence.
ACCESSION   DATKGS010000107 DATKGS010000000
VERSION     DATKGS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63626"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..>2485
                     /gene="rpoB"
                     /locus_tag="VMQ48_02695"
     CDS             59..>2485
                     /gene="rpoB"
                     /locus_tag="VMQ48_02695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HUD08975.1"
                     /translation="MESGVSFMSQEAKTLGVKESFSPQSSLKKRKFFKQLPMVSLPNL
                     IETQTVAYDWFLKDGLRELLDEVNPISDFTGKDLELSFGDYYLDEPKYDEKTSKEKNI
                     SYEAPLRSVVKLLNKQTGEIKDQEVYLGDLPIMTDRGTFVINGVERVVVSQLIRSPGV
                     FFTMDYQKGKKLFGAKIIPNRGAWLEMETDLDGALWVKIDRKRKVAVTSLLRAFGIVS
                     DAEIVKTFADVNKGETNYVEATIERDAAKTQGDGFKEIYKRIRPGDLASVENAKQMIE
                     SMFFNFERYDFGKVGRYRLNQRLEDDAPNDEEHRILRPKDLVLIIKEVIRLNNDPMAE
                     PDDIDHLGNRRVRCVGELVQNKFRAGLARMVRNIKDRMSTCDLATVVPGQLINSRPVA
                     AAVKEFFASSQLSQFMDQVNPLAELEHKRRLSAMGPGGLTRERASFEVRDVHQSHYGR
                     ICPVQTPEGPNIGLVNHLSCYARINEFGFLETPYRKVIVKDGRAFVTKEIDYLNATVE
                     DRKSIAHADIDINQEGYILDEKVEARVKGKPSIIEVQKLDYVDVSPKQCISIATSLIP
                     FLEHDDAHRALMGSNMQRQAVSCILPDSPLVGTGLEDKAAADSGQVILAKEDGEVVEV
                     DASHITMKVEKENGKSVKTNYWLQNFERSNAFTCMNQIPRVATGDRIAKGQLLADGAS
                     TDNGELALGQNVLVALMPWEGSNFEDAIIISERLLQDDRYTSIHIEDFSVDIRDTKLG
                     PEVVTRDIPNIGEERLKNLDDTGVITIGAEVSSGDILVGKISPKGEGDLTPEERLLRA
                     IFGEKSKDVKD"
BASE COUNT          680 a          564 c          694 g          547 t
ORIGIN      
        1 cttctaataa accacttagc caaaatcagc cgcttcccag aaattttcga agcgctgatt
       61 ggagagtggc gtgtctttta tgtcgcaaga ggcgaaaact ctcggggtga aagagtcttt
      121 tagcccgcag tcctctctca aaaagaggaa atttttcaaa cagcttccaa tggtgtcgct
      181 cccgaacctg atcgaaacgc aaacagtcgc ctatgactgg tttctcaaag acggtttgcg
      241 cgaacttctt gacgaggtga atccgatcag cgatttcacg ggaaaggatc tggaactctc
      301 gtttggggac tactatctcg acgaaccgaa atacgacgaa aaaacttcca aagaaaaaaa
      361 catctcttac gaagcgcccc ttcgctcggt ggtgaagctt ctcaacaagc agacgggaga
      421 gatcaaggat caagaggttt atctcggtga ccttcccatt atgaccgacc ggggaacttt
      481 tgtgatcaac ggcgtggaaa gagtcgtggt gagccagttg atccgtagcc cgggagtttt
      541 cttcacgatg gactatcaga aaggcaagaa acttttcggc gcgaagatca ttccgaaccg
      601 cggggcgtgg cttgaaatgg aaacggatct ggatggagcc ctttgggtga aaatagaccg
      661 caaaagaaaa gtggccgtga cgtcgctttt gcgggctttt ggcattgtga gcgacgcgga
      721 aatcgtgaaa acttttgccg atgtgaacaa gggagagaca aactatgtcg aagcaacgat
      781 tgaaagggac gcggccaaga cgcagggaga cggcttcaaa gaaatataca aaaggatccg
      841 gccgggagat ctggcttcgg ttgaaaacgc gaagcagatg atcgagtcga tgtttttcaa
      901 cttcgaaaga tatgactttg ggaaagtcgg ccgctatcgg ctcaaccaac gtttggaaga
      961 tgacgcgcca aatgatgaag aacatcgtat tttgcgaccc aaagatctgg ttttgatcat
     1021 aaaggaagtg atccgtctca acaatgatcc aatggccgag cctgatgata tcgaccactt
     1081 gggaaatcga cgtgtgcgtt gcgtgggcga attggttcag aacaaatttc gggcgggact
     1141 ggcgcgcatg gtcagaaata tcaaggaccg gatgagtacc tgcgatctgg cgacggttgt
     1201 tccggggcag ctcatcaatt cgcgaccggt tgcggcagcg gtcaaagaat tttttgcttc
     1261 ttcgcagctt tcccagttca tggaccaagt caatcctttg gcggaactcg agcacaaacg
     1321 gcgcctttcc gcgatgggac ccggcggtct cacccgcgaa cgggccagtt ttgaagtgcg
     1381 cgatgtccac cagtcgcact atggccgcat ctgcccggtg cagacgccgg aagggccgaa
     1441 catcgggctg gtgaaccatt tgtcctgcta cgcccgtatc aatgaatttg gatttttgga
     1501 aacgccgtat cggaaagtga tcgtgaagga cggacgggct tttgtcacga aggaaatcga
     1561 ctatctcaac gccaccgtcg aagatcgaaa aagtattgct catgccgata ttgatatcaa
     1621 ccaggaagga tatattttgg atgagaaagt tgaggcgcgc gtgaaaggca agccgtcaat
     1681 cattgaagtc caaaaactcg attatgtcga cgtgtcgccg aagcagtgca tttcgatcgc
     1741 gacttcgctc attcctttcc tcgaacacga tgacgcccac cgggctctga tgggttcaaa
     1801 catgcagcgc caggcagtgt cttgcatttt gccggactct ccgctggtcg gaacggggct
     1861 ggaagacaaa gcggcggctg attcaggcca ggtgattttg gcaaaagaag acggggaagt
     1921 ggtggaggtg gatgcgagcc atatcacgat gaaggttgaa aaggaaaatg gaaaatcagt
     1981 caaaacaaat tattggcttc aaaattttga gaggtcaaac gccttcacct gcatgaacca
     2041 gattccgcga gtcgcaacag gggacagaat cgccaaaggt caattgctgg cggatggggc
     2101 ttcaacggac aatggagagt tggcgctcgg acagaacgtg ctggtggcgc tgatgccctg
     2161 ggaaggatcg aactttgaag acgccatcat catttccgaa cggttgcttc aagacgatcg
     2221 ctatacttcg attcacattg aagatttttc ggttgacatt cgcgatacga aactcggacc
     2281 ggaagtagtc acgcgggata ttccaaacat cggagaagag cggctcaaaa atctggacga
     2341 taccggcgtc atcaccatcg gtgcggaagt ttcttcaggc gacattttgg ttggaaaaat
     2401 ttccccaaaa ggagaaggag acctcacacc ggaagaaagg ttgctgcggg cgatctttgg
     2461 agaaaaatca aaagacgtga aagac
//
LOCUS       DATKGS010000108         7829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_54290, whole genome shotgun sequence.
ACCESSION   DATKGS010000108 DATKGS010000000
VERSION     DATKGS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54290"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VMQ48_02700"
     CDS             <1..628
                     /locus_tag="VMQ48_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08976.1"
                     /translation="IDVLEKHPGAKIMYNTLCSKVVEDTIKKFGGEPFMWRTGHSFLK
                     KKNQEVKAAFIGELSGHFFFSADFYNHDDGCYTTLRLLDYLSRSGQTLAEAVAALPKY
                     ISSPEIKLGCADDKKVALIEKMSEVLKKDFPEAEVIDDQRAGDGVRLNLSDSMFVVRY
                     SQNGPYLTIKFEAQTQEKYNELKNYLKNILHKYEEVNWSEGVNVESLE"
     gene            complement(724..1704)
                     /gene="trpS"
                     /locus_tag="VMQ48_02705"
     CDS             complement(724..1704)
                     /gene="trpS"
                     /locus_tag="VMQ48_02705"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HUD08977.1"
                     /translation="MKQRIFSGIQPSGTLHIGNYLGAVKNWVALQEKYDSIFCVVDLH
                     AITVPQDPEALRQKTLELAKIYLAAGINTQESTIFVQSHIPEHTELAWILNTITKIPE
                     LERMTQFKDKAKEQRQNSNAGLFNYPVLMAADILLYDTNVVPIGEDQLQHVELARTLA
                     KRFNQQFGETFVVPEPYIVKEGMRIMGLDDPSKKMSKSAASELNYIALTDTPEKIREK
                     IKKAVTDSGKNVRYADDKPAIKNLINIYSLLDNVKPEEVEKIYKGKSYSEFKADLAEV
                     IVDFLKNFQEKYDAIDDNEVLKMLETGKEKAKKMAEAKMNEVREKVGLLM"
     gene            1815..2168
                     /locus_tag="VMQ48_02710"
     CDS             1815..2168
                     /locus_tag="VMQ48_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUD08978.1"
                     /translation="MLRFIIHILSNAIGIWSSARLVHGIHFHGNWKWLILAGAVMGFI
                     NFFIKPVVKIVSLPLIWLTLGFFTIIINILMLDLVAHIVPALVIETWTAAFWAVIIIS
                     GINFIVSALIKEENA"
     gene            2161..2394
                     /gene="secG"
                     /locus_tag="VMQ48_02715"
     CDS             2161..2394
                     /gene="secG"
                     /locus_tag="VMQ48_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HUD08979.1"
                     /translation="MLENILIWAQLIVSVLLAISILLQNRGAGLSASFGGDFGGYYTK
                     RGMEKFLNWGAIALAVIFIGLAITNIYLGSRSV"
     gene            2403..4085
                     /locus_tag="VMQ48_02720"
     CDS             2403..4085
                     /locus_tag="VMQ48_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HUD08980.1"
                     /translation="MNKIFWQKFFRVFSAKERMTVLSLVLLVVVSLVATVLGVYYSLT
                     KPVPIPGGDYSEGIIGQPMYVNPVLEAGNEADADLSRLIYSGLFKYGTDGKIVPDLAE
                     SYEVSNDQLTYTVHLKKDVKWHDGEAFSSDDVLFTIQAVQDPAFKSPLRQNWQGVGME
                     QVDDSTVKFVLPAPYIFFLNNLTVGIIPKHVWETVAPTNFSLAEYNLKPVGTGPFEYS
                     DFQKDSGGMILSYKLISNPDYFGDKPYLGSMTFQFYPDEDSMIEDYNNKKILATNSLS
                     PEKLNDIKSKRSANIISASLPRYFAVFFNQQKSKVLSDKDVRKALATATNRKDIVDKI
                     FGGQGREIFSPIPPGTFGATDDVKKFDFNPEAAKKILDDAGWKSGSDGMRAKNGQALQ
                     FTLVTTDWPQLVDTANILKSQWEAVGAKINIDSESIGDVQQNFLRPREYEAFLFGQSW
                     PTAEPDPYSFWHSSQTKDPGLNLALYSNPNVDKILEKLRTETDEGKRVDLYKNFQQSV
                     TDDIPAVFLYSPNYLAVVNKKVGGINITSLVSSDEKFADAAKWYVNTKRVRK"
     gene            4099..5127
                     /locus_tag="VMQ48_02725"
     CDS             4099..5127
                     /locus_tag="VMQ48_02725"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HUD08981.1"
                     /translation="MQNKIDKENLYDVIKNIPDQLLTGLEIAKNVKVEGKFKSIMISG
                     MGGSALPGNLLRIYLHDLFFKNSAANHRFGIYQNRNYTLPPESFDNCLNIISSYSGNT
                     EETVSSFEEVLKNNLPCVGIAAGGKVAEMCKGSNVPLVLMPPPEKILQPRMALACNFA
                     AVFQALVNSGMIEDKKKEFEQAVIKLKDSGQIFEELGKKIAEEVAGKTPIVYSSTKFK
                     SLAMIWKIMFNENAKTPAFWNFFPELNHNEMIGFTNPQGKFHLIILRDRQVDPRILKR
                     MEITSGILKKYGVDSTTVDMPEDGILFRIFATLQIGCWASYYLALKYEIDPTPVDMVE
                     ELKVQLTK"
     gene            5255..5338
                     /locus_tag="VMQ48_02730"
     tRNA            5255..5338
                     /locus_tag="VMQ48_02730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5289..5291,aa:Leu,seq:cag)
     gene            5387..7333
                     /locus_tag="VMQ48_02735"
     CDS             5387..7333
                     /locus_tag="VMQ48_02735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HUD08982.1"
                     /translation="MKKVTRPVDGIVSQPSQSSVPSSRRRRNFGSRKRTGSTSAQQSP
                     LRREVVGQIQANKDVSKTASSFAYKKSSQRPRRNVPGKALPGKAAVENKNALRIIPLG
                     GQEEVGRNMTLFEYGGDIIILDMGLQFPEEDMPGIDYIIPNTSYLKGKEKNIRGVILS
                     HGHLDHIGAAPILLKKLNYPPIVGRDLTLALVKKKMEDFEKNSAQNLKTMRVNSVSDK
                     IRLGKFEISFFDVEHSIMDAVGVIIKTPSGTVIHPGDWTMDKDPINGKTITYTHLAKL
                     PSPRILMLESLGAVDTRPAATGEKEMYKNLEKLISDAKGRVIIGTFSSQIRRIGHLIE
                     FAEKIGKKVALDGYSMKMNVEIAKELGYIKAHKETLIPISEASNYPDNRIIVICTGAQ
                     GEGNAVLSRIITDQHRYVKINKDDTVIFSSSVIPGNERTIQRLKDNLYRKSDNVIHSD
                     IMDVHTSGHCNASDIKDILKQIRPDYFLPVYAHHYMLKEAARLARDIGFRRENIFVLD
                     NGMPIEFEKGKGKILPRKVDTSYVMVDGLGVGDIGQVVLRDRQVLAQDGMFTIVTIID
                     SKTKRVIGEPQVTSRGFIYVKENFDLVNATKRKVKDVVAHATAKDESVDWNYVKDQIR
                     DTVGQFLYQKTERRPMILPVVIEV"
     gene            7469..>7829
                     /locus_tag="VMQ48_02740"
     CDS             7469..>7829
                     /locus_tag="VMQ48_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUD08983.1"
                     /translation="MANKSHLIVKGARVNNLKNISVKIPRDKIVVITGLSGSGKSSLA
                     FDTIFAEGHRRYVENLSAYARQFLASVKKPEIEKIENLSPVIAIDQNSVNRSPRSTVG
                     TMTELYDWLRILFARLGK"
BASE COUNT         2140 a         1743 c         1917 g         2029 t
ORIGIN      
        1 aatcgatgtg cttgaaaaac acccgggcgc gaagattatg tacaatacgc tttgctcgaa
       61 agtggtggaa gatacaatca aaaaattcgg cggcgagccg ttcatgtggc ggacgggtca
      121 ttcatttctc aaaaagaaaa accaggaagt gaaagccgcc ttcatcggcg aactttcggg
      181 gcactttttc ttctcggctg atttttataa ccacgatgac ggatgctata cgaccctgcg
      241 gcttctcgac tatctttccc gaagcggaca aacgctcgcg gaggccgtcg cagctttgcc
      301 aaaatatatt tcgagtcccg aaatcaaatt gggatgcgcg gacgacaaaa aggtggcgct
      361 catcgaaaaa atgagcgaag ttttgaaaaa agattttccc gaagccgaag tgattgatga
      421 ccagcgggcg ggcgacggcg tgcgcttgaa cctttctgat tcgatgtttg tggtgcgcta
      481 ttcccaaaat ggcccctatc tcacgatcaa attcgaagcg caaactcaag agaaatacaa
      541 cgagctcaaa aattatctca aaaatatttt gcacaaatac gaagaggtta attggagcga
      601 aggagtgaat gtggaaagtt tggaatagca ataaaatatt ctacaaatag aaaagcccgc
      661 ggctgagatt cccgggcttt ttaaaatccc ccttagtccc cctttagaaa agggggagat
      721 atttcacata agcaagccaa ccttttctct gacttcattc attttcgctt ctgccatttt
      781 tttggctttt tccttgcctg tttctagcat tttcagaact tcattatcgt caattgcatc
      841 gtatttttcc tggaaatttt tcaaaaaatc gacgatcact tcggcgaggt cggccttgaa
      901 ttcggaatag cttttaccct tgtatatttt ctcgacttct tcgggcttca cgttgtcgag
      961 aagggaatag atattgatca aattcttgat ggccggtttg tcatcggcgt aacggacatt
     1021 ttttccgctg tccgtgacgg cttttttgat cttttcgcgg attttttcgg gagtgtcggt
     1081 gagcgcaata tagttcaatt ccgaggcggc cgattttgac atttttttcg agggatcgtc
     1141 gagtcccatg atgcgcattc cttctttgac gatatatggt tcgggtacga caaaagtttc
     1201 gccgaactgc tggttgaatc ttttggcgag cgtccgggcg agttcgacgt gctggagctg
     1261 gtcttcgccg atcgggacga cgttcgtgtc atagagaagg atatcggcgg ccatcagaac
     1321 cggatagttg aaaagtccgg cgttggaatt ttggcgttgc tctttggcct tgtccttgaa
     1381 ttgagtcatc cgttcgagct ccggaatttt ggtgatcgtg ttcaagatcc aagccagctc
     1441 cgtgtgctcg ggaatgtgcg actgaacaaa aattgtcgat tcctgcgtat tgatacctgc
     1501 ggcaagatat attttcgcga gttccaaggt tttttggcgg agcgcttccg gatcctgggg
     1561 gacagtgatg gcgtgaagat cgacaacgca gaaaatagaa tcatattttt cttgaagagc
     1621 gacccagttt ttcaccgcgc cgagataatt tccaatgtgg agcgttccgg aaggttggat
     1681 gcctgaaaaa atacgttgtt tcatataggc ttgattatta gtgttagcta gggttataat
     1741 aggatttaga gagaagaatg tcaaaatttg acgcccaaat tattaattgc tagtatttcc
     1801 caagccttca atctatgcta cgcttcatca ttcacatcct ttccaatgcc atcggcattt
     1861 ggtcttccgc gcgtctcgtg catggcattc attttcatgg caattggaaa tggcttattt
     1921 tggccggagc ggtgatggga tttatcaact ttttcataaa gccggttgtg aaaattgttt
     1981 cccttccgct gatttggctg acactcggat ttttcacgat tattataaat attttgatgt
     2041 tggatctcgt agcgcatatt gttccggctc tcgtgatcga aacctggaca gcggcttttt
     2101 gggcggtgat catcataagt ggcataaact ttattgtttc agccttaatc aaagaagaaa
     2161 atgcttgaaa acatccttat ctgggcgcaa ctgatcgttt cagtgctcct ggcgatttca
     2221 attcttctcc agaaccgggg ggcgggactg tccgcctctt ttgggggcga ctttggagga
     2281 tattatacca agcggggaat ggaaaaattt ctcaattggg gagcgatagc tctggctgtc
     2341 atatttattg gattggctat tacgaacatt tatctgggtt ccagaagcgt ttaacgcaat
     2401 caatgaataa gatcttttgg caaaaatttt ttcgggtatt ttctgcaaaa gagcgcatga
     2461 cggttttgtc actggtgctc ttagtggtcg tttctctcgt ggcgacggtg ctgggggttt
     2521 actattctct caccaagccg gttcctattc ccggcggaga ctattccgaa ggcatcatcg
     2581 gccagccgat gtatgtcaac ccggtgctgg aggccggcaa tgaagccgat gctgatttgt
     2641 cccgtctcat atactccggc cttttcaaat atggaacgga cggaaaaatc gtcccggatc
     2701 ttgccgaaag ttacgaagtt tcaaacgacc agctgacgta tacggtccat ctcaaaaaag
     2761 atgtgaaatg gcacgacgga gaagcttttt cgtcggatga tgttcttttc accatccagg
     2821 ctgtccaaga tccggcgttc aagagtccct tgcgccaaaa ttggcaggga gtggggatgg
     2881 aacaggttga cgacagtacg gtcaaatttg ttctgccggc accgtacatt ttttttctca
     2941 acaatctcac cgtcgggatc attccgaagc acgtttggga gactgtcgcg ccgacaaact
     3001 tttctctcgc ggaatacaac ctgaagccgg tcggaacagg cccttttgaa tactctgatt
     3061 ttcaaaaaga ctcgggcgga atgatcttgt cgtataagct catttcaaac cctgactatt
     3121 tcggcgacaa gccgtatctt ggctcaatga ctttccagtt ctatcccgac gaggattcga
     3181 tgattgagga ttacaacaac aagaagattt tggcgacaaa ctctctttca ccggaaaaat
     3241 tgaacgatat caagagcaag cgcagcgcga acatcatctc ggcgagcctg ccgcgctatt
     3301 tcgccgtttt tttcaatcag caaaaaagca aggtgctctc ggacaaggat gtgaggaaag
     3361 cgcttgcgac ggcgacgaac cgcaaagaca tcgtcgacaa aatattcggg gggcagggac
     3421 gcgaaatttt ttcgcccatt cctcccggga catttggcgc gacggatgat gtgaaaaagt
     3481 ttgatttcaa cccggaagcg gccaaaaaaa ttctcgacga cgccggctgg aaaagcggca
     3541 gtgacggaat gcgcgcaaaa aacggacagg ctctccagtt cacactcgtc acgaccgatt
     3601 ggccgcagtt ggttgatacg gccaacatcc tgaagagtca atgggaagcg gtcggcgcga
     3661 aaatcaatat tgattcggaa agcatcggag atgtccagca gaattttctc cgtccccgcg
     3721 aatacgaagc ttttcttttc gggcaaagct ggccgacggc cgaaccggat ccctattctt
     3781 tttggcattc ttcacaaaca aaagatccgg gactgaacct ggcgctctat tcgaatccga
     3841 acgtagacaa aattctcgag aagttgcgca ctgaaacgga tgaaggaaaa cgcgttgacc
     3901 tttacaaaaa cttccagcag tcggtgacgg acgatattcc ggccgtattt ctctatagtc
     3961 cgaactatct ggctgtggtc aacaaaaaag tggggggaat aaacataaca tcgctcgttt
     4021 cgtcagacga aaaattcgcc gatgccgcca agtggtacgt gaatacgaaa agagtgagaa
     4081 aataaatttc aaaaaattat gcaaaacaaa atcgacaagg aaaatctcta tgatgtcatc
     4141 aaaaatatcc ctgatcagct tctgaccgga ctcgagattg ccaaaaatgt aaaagtggaa
     4201 ggaaaattca aatctattat gatctccggg atgggcggtt cggcgctgcc ggggaacttg
     4261 ctgcggattt atctccatga cctttttttc aaaaattccg cagccaatca tcgctttgga
     4321 atttatcaga atcgcaacta tactttgccg ccggaatctt ttgataattg tttgaacata
     4381 atttcttctt attctgggaa tacagaagaa acagtctcgt cttttgagga agttttgaaa
     4441 aacaatttac cttgcgttgg aatcgcggca ggtggaaagg tagcagagat gtgtaagggc
     4501 agcaatgttc cgttggtgct gatgccgcct cccgaaaaaa ttcttcagcc gaggatggcg
     4561 cttgcttgca actttgccgc ggtgtttcag gctctggtga attccggaat gattgaggat
     4621 aaaaagaaag aatttgagca agcggtgatt aaattgaaag atagtggcca aatattcgaa
     4681 gaacttggga aaaaaatcgc cgaagaggtt gcgggcaaga cacccatagt ctatagttcg
     4741 accaaattca aatcgctcgc gatgatttgg aagattatgt tcaatgaaaa tgcgaagaca
     4801 cccgcgttct ggaatttctt tcccgagctc aatcacaatg aaatgatcgg tttcacgaat
     4861 ccgcagggaa aatttcattt gataatttta cgtgatcgac aagttgatcc tcgtattctg
     4921 aagagaatgg aaatcacttc aggtattttg aaaaaatatg gcgtcgattc aactaccgtc
     4981 gacatgcccg aagatgggat acttttccgt atctttgcca cattacagat cggttgctgg
     5041 gcatcatact atttggcgct caaatatgaa atcgatccca cgccggtgga tatggtggag
     5101 gaattgaagg tgcagttgac aaaatagccg attgggtata taattggaca attggagtgc
     5161 cttcttcttg atttatctaa aatatctctt atctaagcct ttatttatca atctaagacc
     5221 gcattagtgc ggttgctgta agtttttgtt tcatgccgaa gtggtgaaat tggcatacac
     5281 gcagcgttca ggtcgctgtg ggggcaaccc cgtggaggtt caagtcctct cttcggcaca
     5341 aatataatca atcttctaaa ctagaattaa tcaaatcaaa aaaattatga aaaaagttac
     5401 cagaccggtt gacgggattg tttcgcaacc tagtcaatca agcgtccctt cttcccggag
     5461 aaggcgaaat ttcggttcgc gaaaaagaac cgggagtacc tctgctcagc aaagtccgct
     5521 tcgacgtgaa gtggtcgggc aaatacaagc aaataaagac gtttccaaaa cagcgtcctc
     5581 ttttgcatac aaaaaatctt cccagcggcc aagaaggaat gttccaggga aggcgcttcc
     5641 cggaaaagcg gctgttgaaa acaagaatgc tcttcgcatc attccgttgg gaggccagga
     5701 ggaagtcggc cggaatatga ccctgtttga atatggaggc gatatcatca tccttgatat
     5761 ggggctgcag tttccggaag aggatatgcc gggaatcgac tatattatcc cgaacaccag
     5821 ctatctcaaa ggcaaggaaa aaaatatccg cggcgtgatc ttgagtcacg gccatctgga
     5881 ccatatcgga gcggctccta tcttgctcaa aaaattgaac tacccgccga tcgtcgggcg
     5941 ggatctcact ttggctttgg tcaaaaagaa aatggaggat ttcgaaaaga actccgccca
     6001 aaatctcaaa acgatgcggg tgaattctgt tagcgacaag atcagacttg gaaaattcga
     6061 gatcagtttt ttcgatgtgg agcattccat catggacgcg gtgggcgtga ttatcaaaac
     6121 tccgtccgga acagtcatcc atccaggaga ctggacgatg gacaaggatc cgataaacgg
     6181 aaaaacgatc acctatacac atctcgcaaa actgccgagc ccccgcattt tgatgctcga
     6241 gagtttggga gcggtggaca cgcgtccggc ggcgacgggt gaaaaggaaa tgtacaaaaa
     6301 tcttgaaaaa ctcatctcgg acgccaaagg ccgggtgatc attggaactt tttcttccca
     6361 gatccggcgc atcggacacc tcatcgagtt tgctgaaaag atcgggaaaa aagtggcgct
     6421 cgacggatac agcatgaaaa tgaacgtgga gatcgccaaa gaactgggat atatcaaagc
     6481 gcacaaagaa acactcattc cgatttcgga agcgagcaac tatccggaca accggatcat
     6541 cgtcatttgc accggcgcgc agggagaagg aaacgccgtc ctttcgcgga tcatcaccga
     6601 ccagcatcgg tatgtgaaaa tcaataaaga cgacacagtc atcttttctt cctccgttat
     6661 tccgggaaac gaacggacta tccaacgcct caaggacaac ctatatcgca aaagcgacaa
     6721 tgttattcac agtgatataa tggacgtcca taccagcgga cattgcaacg cttcggacat
     6781 caaggacatc ctgaagcaaa tcagaccgga ttattttctt cccgtctatg ctcatcacta
     6841 tatgctcaaa gaagcggcgc ggctcgcgcg ggatatcggt tttcgccggg aaaatatttt
     6901 tgttctcgac aacgggatgc cgattgaatt cgaaaaagga aaaggcaaaa ttctgccaag
     6961 gaaagtcgac acgagctatg tcatggtgga cgggctcggg gtgggcgaca ttggacaggt
     7021 agttttgcgg gaccggcaag ttttggcgca ggacgggatg ttcacgattg tgacgatcat
     7081 cgattccaag accaagcgcg tcatcggcga gccgcaggtg acaagccgcg gattcatcta
     7141 tgtcaaagaa aatttcgacc tggtgaacgc gacgaaacgg aaagtgaagg atgttgtcgc
     7201 gcacgcgacg gccaaagacg agtcggtaga ctggaactat gtcaaagacc agatccgcga
     7261 cacggtcgga caatttctct atcaaaaaac cgaacgccgc ccgatgatcc tgccggtggt
     7321 gatcgaggtg tagaatgacc aatatatagc ataatctatc gagtaaagcc ctgccttgtc
     7381 gcagggcttt actttttttg atttctcctg tacaattcac atctagtttg gtcttataac
     7441 ctcactaaat aatattgtta ataatattgt ggcaaacaaa tcgcatctta tcgtcaaagg
     7501 cgcgcgggtg aacaacctca aaaatatctc agtgaaaatt ccgcgcgaca aaatcgtggt
     7561 gatcaccgga ctttccggtt cggggaaatc atctctggct tttgatacga tctttgccga
     7621 gggacaccga agatacgttg agaatctttc ggcttatgcc cggcagtttc tggcgtccgt
     7681 caaaaaaccg gagatcgaaa aaatcgaaaa tctttcgccg gtcatcgcga ttgaccagaa
     7741 cagtgtgaac cgcagcccgc gttcaaccgt cgggacgatg actgaactct atgactggct
     7801 gcgcatcctt tttgcgcggc tcggcaaac
//
LOCUS       DATKGS010000109         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_135405, whole genome shotgun sequence.
ACCESSION   DATKGS010000109 DATKGS010000000
VERSION     DATKGS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135405"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="prfB"
                     /locus_tag="VMQ48_02745"
     CDS             <1..698
                     /gene="prfB"
                     /locus_tag="VMQ48_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HUD08984.1"
                     /translation="GTGGTDAQDWAEMLLKMYLRFAERKNWKAKIISESRGQEAGIKS
                     ATIEVIGKLAYGYLRVEAGVHRLVRLSPFNAKNLRQTSFALVEVLPEIDKIKEVEIDP
                     GELRIDTFRSGGAGGQSVNKTDSAVRITHIPTGIVASSQNERSQLQNKEQSMKILKAK
                     LHQRYLENKEKEKKEIKGEHTEVQWGSQIRSYVLHPYKLVKDHRTKFESKNPEEVLDG
                     NVEEFIETYLKSK"
     gene            700..1386
                     /gene="ftsE"
                     /locus_tag="VMQ48_02750"
     CDS             700..1386
                     /gene="ftsE"
                     /locus_tag="VMQ48_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HUD08985.1"
                     /translation="MIEFRNVKKIYTGGINTIALDEINFVIEKEEFVSLVGHSGAGKS
                     TLLKLLYAEEKPTSGEIFFNGEDISNLTPKQLPFYRRRIGTVFQDFKLLPRKTLFENV
                     AFALEVSDHSDKEINELVPQILEIVGLAHKLDSYPREISGGEQQRVAIARALVHRPPL
                     IIADEPTGNLDPISSWGIIQLLLKINKLGTTILLATHNKDIVNRVKKRVITLDQGKII
                     RDHSKGRYAI"
     gene            1405..2316
                     /locus_tag="VMQ48_02755"
     CDS             1405..2316
                     /locus_tag="VMQ48_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HUD08986.1"
                     /translation="MLLLTLSRTFKSGFQHFRRNGWLSFAVISIISLTLLIISTLIVL
                     SIAANLVIRSVQDKVDISVYFKSDTEESKIMEFRSTVLQYKEVKSADYVSKNRALDEF
                     KSKNADNPTIMQSIDAIGDNPLNASVNIKAVTPDQYDTIATAVQNSAYKDYISRINYA
                     KNKIVIERLNNVLSTTRNVGAMLATLFSLIAVLITFNAIRITIYAHRQEIEIMRLVGA
                     SNPYIRMPFIFEGIIYGTISAVVVMLFLFPLIKFVAPYLGGAVSVQEVQANFMHNIWL
                     IFLIQLITGVALGTISSLIAIRRYLKA"
BASE COUNT          742 a          623 c          545 g          582 t
ORIGIN      
        1 gcggaaccgg aggaacggac gcacaagatt gggctgaaat gctgctcaaa atgtatctcc
       61 gttttgccga aaggaaaaac tggaaagcaa aaataatttc cgaatcgcgc ggacaagaag
      121 ccgggatcaa aagcgcgacg attgaggtga ttgggaagtt ggcctatgga tatctccggg
      181 tggaagcggg tgtgcatcgt ttggtgcgcc tttctccctt caatgccaaa aatttgaggc
      241 aaacatcttt tgccctggtt gaagttttgc cggaaattga taaaataaaa gaagttgaga
      301 ttgatccggg cgaattgaga attgacacgt ttcgatcggg aggagccggc ggacaatccg
      361 tgaacaaaac agacagtgcc gtacgcatca cacatattcc gacaggaatc gtcgccagtt
      421 cccaaaacga acgcagccaa ctccagaaca aggagcaatc gatgaaaatc ctgaaagcca
      481 agctgcatca acgatatctc gaaaacaagg aaaaagaaaa gaaagaaata aaaggggagc
      541 ataccgaagt ccaatgggga agtcagatcc ggtcgtatgt cctgcatccc tacaaactcg
      601 tcaaagacca ccggacaaaa tttgaatcca aaaatccgga agaagtgctg gatggaaacg
      661 tggaagagtt tatcgaaaca tatctcaaat caaaataaaa tgattgaatt ccgcaacgtc
      721 aaaaaaatat ataccggagg aatcaacacc atcgccctcg acgagatcaa ttttgtcatt
      781 gaaaaagaag aatttgtttc ccttgtcggt cacagcggag cgggaaaatc gactcttctc
      841 aagctgctct atgccgaaga aaaaccgact tcgggagaaa tttttttcaa cggagaagat
      901 atttcaaatc tcacccccaa gcagctgcct ttttatcgcc gccggatcgg aacggtcttc
      961 caggatttca aattgctccc cagaaaaacc ctttttgaaa acgtggcctt tgccttggag
     1021 gtttccgacc atagcgacaa agaaatcaat gaactggttc ctcaaattct ggaaattgtc
     1081 ggcctcgccc acaaattgga cagttatccc cgcgaaatat ccgggggaga gcaacagaga
     1141 gtcgccattg cgagagcttt ggttcatcgg cctccgctca tcattgctga cgaaccgaca
     1201 ggaaaccttg acccgatcag cagctgggga atcattcagt tgcttctcaa gatcaataaa
     1261 ttgggaacga ccatccttct cgccacccac aataaagata ttgtcaatcg cgtcaaaaaa
     1321 agagttatca cgctcgacca ggggaaaata atccgcgacc atagcaaagg ccgctacgcg
     1381 atataatttc gcaaatacaa aataatgtta ctgctcacac tctcacgaac attcaaatca
     1441 ggattccagc atttccgccg caacggctgg cttagttttg ccgtgatcag catcatctcc
     1501 ctcacgcttc tcatcatcag cacgttgatt gttctttcaa tcgccgcgaa tcttgtcatc
     1561 cgatccgtcc aggacaaagt ggacatcagc gtctatttca aaagcgacac ggaagaatcg
     1621 aaaattatgg aattccggtc aactgttttg caatataaag aagtgaaatc agccgactat
     1681 gtttcaaaaa accgagccct cgacgaattc aaatcaaaaa atgcggacaa tccgactatc
     1741 atgcagtcga tcgacgccat cggcgacaat cctttgaatg cttcagtgaa catcaaggct
     1801 gtcaccccgg accaatatga caccattgcg accgctgtcc aaaattccgc ctacaaagat
     1861 tatataagcc ggatcaacta cgccaaaaac aagatcgtga ttgaacggct caacaacgtc
     1921 ctctcaacca cccggaatgt cggcgcaatg cttgcgacac ttttctctct tatcgccgtg
     1981 ctcatcactt tcaatgccat ccgcatcacg atatacgccc accggcagga aattgaaatc
     2041 atgcgcctgg tgggagcctc aaacccctat atccggatgc cgttcatctt tgagggcatc
     2101 atctatggaa caatatcggc cgtcgtcgtc atgcttttcc ttttcccgct gatcaaattc
     2161 gtcgcgccct atcttggcgg cgccgtttcc gtgcaagagg tgcaagccaa tttcatgcac
     2221 aatatctggc ttattttcct tatccaactc atcaccggtg tcgctctcgg aaccatcagc
     2281 agcctcatcg ccatccggag atatttgaaa gcgtaaaaac aaaaacctcc ccctttcgaa
     2341 aagggggatc gagggggatt ttgaaagccg ttacgggctt ttttgctttt tttacttgaa
     2401 aaatttcgcc ttttgagcta caatctattt tgtattgggg tgtagccaag tggtaaggca
     2461 acgggttctg gtcccgtgat cctaggtccg aa
//
LOCUS       DATKGS010000110         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_98996, whole genome shotgun sequence.
ACCESSION   DATKGS010000110 DATKGS010000000
VERSION     DATKGS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98996"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VMQ48_02760"
     CDS             complement(<1..263)
                     /locus_tag="VMQ48_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HUD08987.1"
                     /translation="MKSRKIRYLEKILKYFATKALRRFKPRVVGVTGSVGKTSTKEAI
                     FSVLSSKFRVRKNEKNYNNEIGLPLTILGIESGGGSPWKWVVV"
     gene            complement(396..>2274)
                     /locus_tag="VMQ48_02765"
     CDS             complement(396..>2274)
                     /locus_tag="VMQ48_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUD08988.1"
                     /translation="MVSKKISRHKTGSLKSNLRIYILVFFVFAAAGAILFRLYNLQVT
                     AHAYYEELASNQHKIYEDLVPKRGEIFAKDGSGYYPVAVNRELSLVYAVPKEIEDPRA
                     EAHELASILQLDERDLESKLDQPNGWYAVIQHKVEDDKAAEITSKKLKGIYMSPESER
                     FYPAGNFASQVVGFVGSDGEKTVGRYGLESYWDKELQGTPGSLEQERDTGGRWISISE
                     KNIVPAQNGADLYLTIDHTIQYRAEMAIKKAVEKYQADRGSMVIIDPKTGAVLAMASA
                     PDFNLNDYSQISDMSLFANPAVSDNYECGSIFKSITLASGVDAKVVTPDSTYTDTGAV
                     TEAGYTIMNSDKKANGVQTMTQVLEKSLNTGAIHVEKLLGNMRFLEYVKNFGFGSKTG
                     IDTIGEASGNISGLKELRNINSFTAAFGQGLSVTPIQFASAIAAIANGGELMKPYFVD
                     KVAYPDGHEDDIQPQVVRQVISKEASLDATRMLISVVQNGHGKRAAVPGYLVAGKTGT
                     AQVPKPGGGGYEDNQHIGSFAGYAPAYDPKFVMLVKLDNPKNVDWAESSAAPTFGEMA
                     KFLLDYYNIEPTEQYTQHDVDLFNATHDVSIYQNEAALAPVATPAPQPNDTNKNNKKK
                     H"
BASE COUNT          525 a          593 c          480 g          676 t
ORIGIN      
        1 aatacgacca cccatttcca aggacttcca cctccgcttt caattcccaa aattgtgagc
       61 ggcaagccga tttcgttgtt atagttcttt tcgtttttgc gcacccggaa tttcgaagaa
      121 agcacggaaa aaatcgcttc cttggttgaa gtttttccga ccgatcctgt cacgccgaca
      181 acacgcggct tgaaacgccg cagagctttc gtcgcgaagt atttgagaat tttttcaaga
      241 tagcgtattt tacgagactt catattagaa ttttataatt tcagaatttt caattttcaa
      301 acaatgactg aattattcaa tttcaaaatg tttctgaaat ttcgatcatt tgaaaattgt
      361 ttgaaaattt tggaattttg acattgaaaa ttaacttagt gtttcttctt attattttta
      421 tttgtatcgt tcggctgcgg tgcgggagtg gcaaccggcg ctagtgcagc ttcattttgg
      481 tatattgaaa cgtcatgcgt cgcattgaaa agatcaacat cgtgctgggt atactgctcc
      541 gtcggttcga tattatagta atctagcaga aatttggcca tttcgccaaa tgtcggcgcg
      601 gcgcttgatt ccgcccaatc cacatttttg ggattgtcga gcttcaccaa catgacaaat
      661 tttggatcat aggccggcgc atatccggca aatgaaccaa tatgctggtt gtcttcatat
      721 ccgcctccgc ccggcttggg aacttgcgcc gttcccgttt ttccggccac cagataaccc
      781 ggcacggctg ctctttttcc atgcccgttt tgcacaacgc tgataagcat ccgcgtcgca
      841 tccaaacttg cttccttgga aatgacctgt ctgacaactt ggggctggat atcgtcctcg
      901 tgcccgtcgg gataggctac tttgtccaca aaatacggct tcatcaattc tcctccattg
      961 gcgatcgccg cgatagcgct tgcaaactgg atcggtgtga cggaaagacc ctgcccgaaa
     1021 gccgccgtaa atgagtttat gttccgcaat tccttgaggc cggagatatt tccgcttgct
     1081 tctccgatcg tgtctattcc cgtcttgctg ccaaacccaa aatttttcac atattccaaa
     1141 aatcgcatat tgccaagaag tttttcgaca tggattgctc ccgtgttcaa agatttttcc
     1201 agaacctgcg tcatcgtttg cacgccgttc gccttcttgt cggagttcat gatcgtatat
     1261 cccgcctctg tgaccgctcc cgtgtccgtg tatgttgagt ccggggtcac cactttcgcg
     1321 tcaactccgg atgccagcgt gatcgatttg aaaattgatc cgcattcata gttgtcactc
     1381 accgccggat tcgcgaacaa ggacatatcg ctgatttgcg aatagtcatt cagattgaaa
     1441 tcgggagccg aagccattgc gagaactgca cccgtttttg gatcgatgat caccatgctc
     1501 ccccggtcgg cctgatattt ttccaccgct tttttgatcg ccatttcggc gcggtattgg
     1561 atcgtatggt cgatcgtcaa atagaggtca gcgccattct gggccggcac aatgtttttc
     1621 tcgcttattg aaatccagcg gccgccagta tctctctctt gttccaggct tccaggcgtc
     1681 ccctgcagct ctttgtccca atatgattcc aggccatagc gcccgaccgt tttttcccca
     1741 tccgaaccca caaacccgac cacctgggaa gcgaagtttc cagccggata aaaccgctcc
     1801 gattccggag acatatatat tcctttcaac ttttttgacg ttatttccgc agccttgtcg
     1861 tcttcaacct tgtgctggat cacggcgtac cagccattcg gttgatccaa ttttgattcc
     1921 aaatcacgtt cgtccagttg cagaatggat gccaattcat gcgcttcagc tcttggatct
     1981 tcgatttctt ttggcacggc ataaacaagc gacagctcgc gattgaccgc cacgggatag
     2041 tatccgctcc cgtctttcgc gaaaatctct ccccgcttgg gaaccaaatc ctcatatatt
     2101 ttgtgctggt tggaggccaa ttcttcataa tacgcatgag ccgttacttg caaattatac
     2161 agccggaaga gaatagcacc cgccgccgca aaaacaaaaa aaaccagaat ataaattcgg
     2221 agatttgatt tcagagatcc agttttgtgc ctgcttattt ttttactaac catg
//
LOCUS       DATKGS010000111         4394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_80252, whole genome shotgun sequence.
ACCESSION   DATKGS010000111 DATKGS010000000
VERSION     DATKGS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80252"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VMQ48_02770"
     CDS             complement(<1..216)
                     /locus_tag="VMQ48_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08989.1"
                     /translation="MARKTALIKISGDLLSKKEVIDKIKEISKEYFTVITVGGGTQIS
                     ETFQKKGFLIKFGPLGRETGSLEEKQLA"
     gene            complement(226..933)
                     /locus_tag="VMQ48_02775"
     CDS             complement(226..933)
                     /locus_tag="VMQ48_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD08990.1"
                     /translation="MKKIKVGIIGHTGRLGKPLLEILNGHPFVEVIYTESRSEGAQGN
                     IKETEVVFLALPYGESERYLKKLSGKRIIDLSIDHRNDSGWTYGLSEIFGNKIKKSKL
                     VANPGCYATSILLGLLPLKGKVKSVHISSTSGVSGAGIAVSRDDNFLVYKEGRQHPQI
                     KEIGKVIGLDEILFVPQRIDTAERGIISTMFVQIDGADNISELYEKAYAKHPFVSIKN
                     EIKTKNVVDTNFCYKNF"
     gene            complement(1008..1082)
                     /locus_tag="VMQ48_02780"
     tRNA            complement(1008..1082)
                     /locus_tag="VMQ48_02780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1047..1049),aa:Gly,seq:tcc)
     gene            complement(1095..1165)
                     /locus_tag="VMQ48_02785"
     tRNA            complement(1095..1165)
                     /locus_tag="VMQ48_02785"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1131..1133),aa:Pro,seq:tgg)
     gene            complement(1222..1545)
                     /locus_tag="VMQ48_02790"
     CDS             complement(1222..1545)
                     /locus_tag="VMQ48_02790"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HUD08991.1"
                     /translation="MATDINKEILVDMVSSKNGMAKKDVEIVIDNVVDVITDALHMDR
                     KVTIASFGTFKVTHRAAREGINPQTKQRIMIPAVNTPKFTAGKRLKEMVRSQAVLSAA
                     PSQAE"
     gene            complement(1654..3186)
                     /gene="rny"
                     /locus_tag="VMQ48_02795"
     CDS             complement(1654..3186)
                     /gene="rny"
                     /locus_tag="VMQ48_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HUD08992.1"
                     /translation="MEFSFGLLFIGILALAAGSVLGYFARQSIAKKQLGTAEGKINKM
                     TQEAEEKARLSLIEAKNRAVEVLEEAKKEEKQRQEQIYRLEKRLEGREENLDKKMDEL
                     EKEKNHLISKADEIKRIRKETDAARQEELKRLEKIAGLSKEEAKKILLQLAEEQNREI
                     LAERIKKMEREGQDELDKKARNIMSMAMQRYAGSHAAETTTSTVAIPSDELKGRIIGR
                     EGRNIKTLERLTGIEVIVDDTPEAIVLSGFNPVRREIARIALEKLITDGRIHPTRIEE
                     AIVFAKAEVNNKIKEAGEAAAYDVGVAGLDPKLIQLLGRLRYRTSYGQNVLLHSLEVA
                     HLSGALAAELGANVSLAKKAGLLHDIGKAIDHEVQGTHIEIGVNVLKKFGIGQDVIDA
                     VKSHHEDYPFESNEAMIIAAADALSASRPGARKDTLEDYLKRLGDLENVAQSFPEVEK
                     TYAIQAGREIRVFVKPDKIDDLGAVKLARSIADKIEQELKYPGEIKVNVLRETRAVEI
                     AR"
     gene            3369..>4394
                     /gene="gpmI"
                     /locus_tag="VMQ48_02800"
     CDS             3369..>4394
                     /gene="gpmI"
                     /locus_tag="VMQ48_02800"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HUD08993.1"
                     /translation="MNKPVYLIVLDGWGENSTFEGNAIAQANTPTVDKISQHYPQTLL
                     QASGISVGLPWGEVGNSEVGHLTLGAGRVLYQSLPRITLSIQDGSFFKNESFLAAVEN
                     VKKNSSTLHLMGLVSNGGVHSSMDHLYALLELAKNSGLEKICLHLFTDGRDAPTKSGI
                     KIIQDVEERIKNFPGACIASLCGRYFAMDRNDNWTRTEKAYNLLTLGAGSRQKSAQTA
                     LETSYEKNITDEFIEPVMIEDEKGNISVVGEKDSIIFFNFREDRARQLTKAFALPTFS
                     KFERKNYFPDIEFVTMTKYENDLPVRVAFGPQEIKNCLGEVLSQNNINQLRIAETEKY
                     AHVTYFFN"
BASE COUNT         1081 a         1160 c          850 g         1303 t
ORIGIN      
        1 cgccaattgc ttttcctcga gtgatccggt ttcccttccc agcggtccga actttattag
       61 aaaccccttt ttctggaaag tttcgctaat ctgggtgccg cctcccacag tgataacggt
      121 aaaatattcc ttggagattt cctttatttt atcaataact tcttttttgg acagaagatc
      181 gcccgatatt tttattaatg ccgtttttct ggccatacgc ttgccttaaa aatttttata
      241 gcaaaaattg gtgtcaacga catttttggt tttgatttca ttttttatac tgacgaaagg
      301 atgtttggcg tatgcctttt catataattc cgatatatta tccgcgccat caatctgaac
      361 aaacatcgtt gatatgattc ctctttcggc ggtatcgatt ctttgcggga caaacaaaat
      421 ttcatccaga ccaatcactt ttccaatttc tttgatttgc gggtgctgcc gaccttcttt
      481 atagactaaa aagttatcat ctcttgagac cgctatgcca gcacccgaaa caccgctagt
      541 cgaagatatg tgcacacttt taacttttcc cttcaacgga agcaacccca gcaagattga
      601 tgttgcataa catcccgggt ttgctaccag ttttgatttt ttgattttat taccaaatat
      661 ttccgacaag ccataagtcc atccgctgtc atttctgtga tcgatgctca aatctataat
      721 gcgtttccca ctcaattttt tcaaatatct ttcgctctcg ccgtaaggca acgccaaaaa
      781 cacaacctct gtttctttta tgtttccctg cgctccctca cttctgcttt cggtatatat
      841 tacttccacg aaaggatgcc cgtttaatat ttcgagcagt ggcttcccca atcttcccgt
      901 atgtccgatt atcccaactt ttattttttt catatttcca ttatacccag ttctggatta
      961 acttcctagc aacaaaaaac aaggctcaaa aagagcctca ttcttaatgg agcgggtaac
     1021 gggaatcgaa cccgtatatc gactttggaa gagtcgcata ataaccatta tactataccc
     1081 gcctattaaa cttttcggag tgctgggaat cgaacccagt caacgtgctc ccaaagcacg
     1141 cgtactgccg gtatacgaca ctccgacaat ttgccccaca ttcaaaagac gtggggcaaa
     1201 cttatcgaaa aataatttta tttattcagc ttgggaaggc gcagcgctga gaacagcttg
     1261 tgagcgaacc atctctttga gacgctttcc ggccgtgaat ttcggggtgt tgactgccgg
     1321 gatcataatc cgctgcttgg tttgcgggtt gatgccttcg cgcgccgccc gatgggttac
     1381 tttgaaagta ccgaagcttg cgattgtcac tttgcgatcc atatgaagcg cgtccgtgat
     1441 cacatcgaca acattgtcga tcacgatttc aacatctttc ttggccattc cgtttttgct
     1501 cgaaaccata tccaccaaaa tttctttatt gatatccgtt gccatatttc accccgcttt
     1561 ctatattact ttttagttta tataaagcta aatacattct aatacttact tatattactg
     1621 acaagcatct ctggaaattt caattcctga atcctatctc gcgatttcca ccgctctcgt
     1681 ttcgcgcaaa acattcacct tgatctcgcc cggatatttg agctcttgtt caattttgtc
     1741 ggcaatggac cgcgccaatt tgaccgcgcc caagtcgtcg attttgtccg gcttcacaaa
     1801 aactctgatc tcgcgtccgg cctggatggc gtatgttttt tccacttcag ggaatgactg
     1861 agcaacattt tccagatccc cgagacgctt gagatagtct tcgagcgtat ctttccttgc
     1921 tcccggacga gaggcggaaa gcgcatccgc cgccgcgatg atcatcgctt cattgctttc
     1981 gaatgggtag tcctcgtgat gcgatttcac cgcatcgatc acgtcttgtc cgatcccgaa
     2041 ttttttgaga acgttgaccc ctatttcgat atgcgttccc tgcacttcat ggtcgatcgc
     2101 ctttccaata tcatgcaaaa gtccggcctt ttttgcgaga ctcacattcg cgccgagttc
     2161 cgccgccagt gctccggaaa gatgtgccac ctcaagcgaa tgaagcaata cattttgtcc
     2221 ataactcgta cggtatctaa gccgccccaa gagttggatc aacttcggat caagcccggc
     2281 cacaccgaca tcataggccg ccgcctcgcc cgcttccttg atcttgttgt tcacttcggc
     2341 tttggcaaaa acaatcgctt cttcaatgcg agtcggatgg attctcccgt cagtgatcag
     2401 tttttcaagc gcaatgcggg caatctcccg ccgcaccgga ttgaatccgg acaaaacaat
     2461 cgcttccggc gtatcatcga caatcacttc aatcccagtg agccgctcca gcgttttgat
     2521 atttcttcct tcgcgcccga tgatccttcc cttgagctcg tccgaaggaa tcgccactgt
     2581 tgaagtagtc gtttcggccg catggcttcc cgcgtatctc tgcatggcca tcgacatgat
     2641 gtttcgcgct tttttatcaa gctcgtcttg gccttcacgc tccattttct tgatccgctc
     2701 agccaaaatt tcacgatttt gctcctcggc gagctggaga agaatttttt tcgcctcttc
     2761 ctttgaaaga ccggcgatct tttcaagtcg cttgagttct tcttgccgcg ccgcatctgt
     2821 ttcttttcgg atccgcttga tctcatccgc cttgctgatc aaatgattct tttctttttc
     2881 caactcgtcc attttcttgt ccaaattttc ttcgcgtccc tcgagtcttt tttccaagcg
     2941 ataaatttgc tcttgtcttt gcttttcttc tttttttgct tcttccaaga cttcaaccgc
     3001 cctgtttttc gcttcgatca aagacaaccg agctttttcc tcggcctcct gcgtcatttt
     3061 gtttattttt ccctctgcgg ttccaagttg cttcttagca atcgactggc gggcgaagta
     3121 ccccaagacc gagccggctg ccaaagccaa aatcccaata aaaagaagtc cgaaactgaa
     3181 ttccattttt ttgcgagcta ttcacttgat aacaatttga aatcatctca aatactttat
     3241 aatttgcacg atatattcgc tgatattcct tggttatcaa ttgaagcata attaatatta
     3301 accgagataa atcatatatc atttcacctt ttttgtcaaa gaggctgaaa atgtgataaa
     3361 atcatcatat gaacaaacca gtctatctca ttgtcctcga cgggtggggc gaaaattcca
     3421 cttttgaggg gaacgctatc gcacaggcca acactccgac cgtcgacaaa atatcacagc
     3481 actatccaca gacgctgctt caggcgtcgg gaatttcggt cggcttgccc tggggcgagg
     3541 tgggaaattc cgaagtggga cacctcactt tgggagccgg acgcgtctta taccaaagcc
     3601 ttccccgcat cacactttcc atccaggacg gaagcttttt caaaaacgaa agtttcctcg
     3661 ccgccgttga aaacgtgaaa aaaaacagca gcactcttca tcttatgggt ttagtttcaa
     3721 acggaggcgt acacagttca atggaccacc tctatgccct tcttgaactc gccaaaaaca
     3781 gcggacttga aaaaatttgc ctccatcttt tcaccgacgg ccgggacgca cccaccaagt
     3841 cgggaatcaa aatcattcag gatgttgagg agcggatcaa aaactttcca ggggcgtgca
     3901 tcgcctccct ctgcggacgc tatttcgcaa tggacagaaa tgacaattgg acccggacgg
     3961 aaaaagccta caaccttctg acccttggag cgggatcacg gcaaaaatcg gcgcaaacgg
     4021 cgcttgaaac atcttacgaa aaaaatatca ctgatgaatt catcgaaccc gtcatgattg
     4081 aagacgaaaa gggaaacatc tccgttgttg gtgaaaaaga ttcgattatt tttttcaact
     4141 tccgcgagga tcgcgcccgc cagctcacaa aggctttcgc cttgccgacc ttttcaaaat
     4201 tcgaaagaaa aaattatttc cccgacattg aatttgtgac aatgacaaaa tatgaaaacg
     4261 atcttccggt ccgggtcgcc ttcggaccgc aggaaatcaa aaactgcctc ggcgaggtat
     4321 tgagccaaaa caacatcaac cagctgcgca tcgccgaaac ggaaaagtat gcccacgtca
     4381 cctatttttt caac
//
LOCUS       DATKGS010000112         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_122409, whole genome shotgun sequence.
ACCESSION   DATKGS010000112 DATKGS010000000
VERSION     DATKGS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122409"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2686)
                     /gene="ileS"
                     /locus_tag="VMQ48_02805"
     CDS             complement(<1..2686)
                     /gene="ileS"
                     /locus_tag="VMQ48_02805"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HUD08994.1"
                     /translation="MFNPVNPKQSFPEMEENVLKFWEENKIFEKSLEQRRDAKLYSFY
                     DGPPFATGLPHHGHLLSSTSKDVVPRYWTMRGYNVPRRWGWDTHGLPIENIVEKKFGY
                     EGKKDIEKNIGKFNSEARKTVLQFADEWKKTIRRIGRWVDFDNSYKTMDNDYMESVWW
                     GFNELHKKGLIYKDSRISLYCPRCATPLSNFEIAMDSSYRDVQDASVYFKLKVKSEEY
                     PDTFFLAWTTTPWTLPGNVAIAIKEDGRYAFIKFNKIIRNDAKLSIKEGETYILEIDR
                     IRQLFGINVDSLKTEGQKDIREIENTKSENENLDVEIGYKIIKGKDLIGFEYYPIFPH
                     EIENGYRVVAGDFVSLEDGTGIVHIAPAFGEDDFRTRKENNLPIIENVDDEGKFKDGD
                     WAGENVWEANFKILKRIADQGFVFKKENVTHSYPFCYRCQTKLIYKAQPAWFIKVSDL
                     KEKLLTENEKINWHPAHLKEGRFKNGLETAPDWNVSRSRFWGTPMPIWECEKCKEVKI
                     LGSYKELEELSGAKLEDYHRPGIDEIVFGCEKCDGKMKRIPDVFDCWVESGSMPFAEF
                     HYPFENKEEFEKRFPAQFISEYIAQTRGWFYTLHVLAVGLFGKPSFLNSVTTGTIAGN
                     DGKKMSKSLGNFTDPNILIDRYGADSFRFYLMQSPLMDGENLNFNDEDLKDIFGGMLR
                     MLWNTYSFFVLYANIDKFEPKNSKYEIPENANLLDRWIVSELNSLIKNVNHHMEHYEL
                     NKTARLFPEFIDNLSNWYVRRSRKRFWKSENDGDKNEAYETLHHVLVELSKLMAPFTP
                     FVAEEIYKNLTGNESVHLADFPVADKKLIDDDLNEKMQLTRRFVKMGLAARAKVGIKV
                     RQPLAELKINEDIGGDLGELIKEEVNVKKISFSGGVALP"
     gene            complement(2679..>3509)
                     /locus_tag="VMQ48_02810"
     CDS             complement(2679..>3509)
                     /locus_tag="VMQ48_02810"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUD08995.1"
                     /translation="PASYRGKIVTTFYDFAPYRLPELFPRLSTAKLKTLYSFAAKKSD
                     QIITVSESTKYDAKELLRFPEEKITAIYNGIDKRFFEEGSFSRDDVKKNYGIIDKYIL
                     FLGTLEPRKNLSRVLDAFARFKKNYKNSSDYQLVVAGKKGWLTEEYFQQTKDLGIEDD
                     VIFTGYVGGDELKPLYTNAEFFVMPSLYEGFGQTVVEAMACGAPCLVSKVSSIPEIAD
                     EAAYFVDPHDTDGIAKGMTELASNQELREKLANAGKQQAKKFSWEKCARETLEVYKNV
                     "
BASE COUNT          897 a          858 c          668 g         1086 t
ORIGIN      
        1 ggggaagagc aacaccgccg gaaaagctga tttttttgac attaacttct tctttgatga
       61 gctcgcccaa gtcccccccg atatcttcgt tgatcttgag ttccgcgaga ggctggcgaa
      121 ctttgattcc gacttttgcc cgcgccgcga gacccatctt cacgaaccgt cttgtcagtt
      181 gcattttttc gttgaggtcg tcgtcgatca atttcttatc ggctaccgga aaatcggcga
      241 ggtgcacaga ttcattgcct gttaaatttt tataaatttc ttccgcgacg aacggggtga
      301 acggcgccat caacttcgag agctcgacaa ggacatgatg cagagtttca taggcctcgt
      361 ttttgtcccc gtcgttttcg cttttccaaa atcttttccg gctgcgacgg acataccagt
      421 tggaaagatt gtcgatgaat tccggaaaca gccgcgccgt cttgttgagc tcatagtgct
      481 ccatgtgatg attcacgttc ttgatcaaac tgttgagttc cgaaacgatc cagcgatcca
      541 gaagattcgc attttccgga atttcatatt tgctattttt cggttcaaac ttatcaatat
      601 tcgcgtacag cacgaaaaaa gaatatgtat tccacagcat ccggagcatc ccgccaaaaa
      661 tatcttttag gtcctcgtcg ttaaagttta agttttcccc atccataaga ggcgattgca
      721 tcagataaaa tcggaaagaa tcggcgccgt aacggtcaat taaaatattc ggatcagtga
      781 aatttccaag cgacttcgac attttctttc cgtcatttcc ggcgatagtt ccggttgtaa
      841 cggaattcaa aaagctcggc tttccgaaaa gtcccactgc aagaacgtgc agtgtataaa
      901 accatcctcg tgtttgagca atatactcgg aaataaactg cgccggaaat cttttttcaa
      961 actcttcctt gttttcaaaa ggatagtgaa attcagcaaa cggcatcgaa cccgattcaa
     1021 cccagcagtc aaaaacatcc ggaattctct tcattttccc atcgcatttt tcacacccaa
     1081 atacaatttc gtctatacct ggccggtgat aatcctccag tttcgctccc gacaattcct
     1141 ccagttcttt gtacgatcct aaaattttaa cctctttgca tttttcgcat tcccaaatcg
     1201 gcattggagt tccccaaaaa cgggaacgag aaacattcca atcaggagct gtttcaagac
     1261 cattcttaaa tcttccctct ttgagatgcg caggatgcca gtttattttt tcgttttcag
     1321 taagtaattt ttccttgaga tcggaaacct tgatgaacca cgccggttga gccttgtata
     1381 ttagtttcgt ctggcagcga taacagaacg ggtaagagtg agtgacattc tcttttttaa
     1441 aaacaaatcc ctggtcggca atgcgcttca gtattttgaa gttcgcttcc caaacatttt
     1501 ctccggccca gtcgccatcc ttgaattttc cttcatcgtc tacgttctcg atgatgggca
     1561 gattattctc ttttcgcgtt cgaaaatcat cttctccaaa agccggtgca atatggacaa
     1621 ttcctgttcc gtcctcgaga ctcacaaaat caccagccac aactctgtat ccattttcta
     1681 tttcatgcgg gaaaatcgga taatattcaa aaccaatcag gtcttttccc ttaataattt
     1741 tatatcctat ttcgacatct aggttttcat tttcagattt cgtattttca atttctctaa
     1801 tgtctttttg tccttcggtt ttcaaagagt caacattaat gccaaatagc tgcctaattc
     1861 tatcaatttc caaaatatat gtttcaccct cttttatgct taactttgca tcatttctga
     1921 tgatcttatt aaattttata aatgcgtatc ttccatcttc cttgattgcg atcgctacat
     1981 ttcccggcag tgtccagggc gttgttgtcc aagcaagaaa aaaagtatct ggatattcct
     2041 cacttttgac ttttagctta aaatacacag aggcatcctg aacatcccga tagctggaat
     2101 ccatcgcgat ttcaaaattt gaaagcggcg tagcgcaacg cggacaatac agagaaattc
     2161 tggaatcttt atatattaga ccctttttat gcaattcatt aaacccccac cagaccgatt
     2221 ccatataatc gttgtccatg gtcttgtagg aattgtcaaa atcaacccaa cgcccgattc
     2281 gtcgaatggt ttttttccat tcatcagcaa attgaaggac cgtttttctg gcttcactgt
     2341 taaacttgcc aatatttttt tcaatatctt ttttcccttc atatccgaat tttttctcga
     2401 caatattttc aataggcagc ccgtgcgtat cccatcccca gcgacggggc acgttgtatc
     2461 cgcgcattgt ccaatatctc ggcacaacat cctttgatgt cgacgacaaa agatgaccgt
     2521 gatgaggcaa tcccgtcgcg aatggtggac catcataaaa agaatagagc ttcgcgtccc
     2581 tgcgttgctc caaagatttt tcgaatatct tgttctcttc ccagaatttg aggacatttt
     2641 cctccatctc gggaaaagat tgtttcggat tgacgggatt aaacattttt gtacacctcc
     2701 aaagtttctc tggcgcattt ttcccagctg aattttttgg cctgctgttt tccggcattt
     2761 gccaattttt cacgcagttc ctgattcgac gccagctccg tcattccttt cgcgattcca
     2821 tccgtatcat gcggatcgac gaaataggct gcttcatccg cgatttccgg aatagaggaa
     2881 actttcgaca ccaggcaggg cgcgccgcaa gccatcgctt cgacgaccgt ttggccaaac
     2941 ccttcataga gcgagggcat cacaaaaaat tccgcgtttg tataaagcgg cttaagctcg
     3001 tctcccccga catagccggt gaaaataaca tcgtcttcaa tccccaagtc tttcgtctgc
     3061 tgaaaatatt cttccgtgag ccagcctttc ttccccgcca cgaccagctg atagtcagaa
     3121 ctgtttttat aatttttctt aaaacgggcg aaggcatcga gcacccggct caagtttttg
     3181 cgcggctcga gcgtccccag aaacaaaata tatttgtcga tgatcccata attctttttc
     3241 acatcatctc ttgaaaaaga gccttcttca aaaaatctct tgtcgattcc gttgtatatt
     3301 gccgtgatct tttcttcagg gaatctcaaa agctcttttg cgtcatattt ggtcgattcg
     3361 gaaacggtga tgatctggtc tgattttttg gcggcaaagg aatagagtgt tttcagtttt
     3421 gccgtcgaaa gacgcggaaa aagttccggc aagcgatagg gagcaaaatc atagaaagtg
     3481 gtcacgattt ttccgcgata gctcgccgg
//
LOCUS       DATKGS010000113         2718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_194485, whole genome shotgun sequence.
ACCESSION   DATKGS010000113 DATKGS010000000
VERSION     DATKGS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194485"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VMQ48_02815"
     CDS             <1..353
                     /locus_tag="VMQ48_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HUD08996.1"
                     /translation="KLVKLDILVAGDKIDAFSRIVPEERAFSEGKATVEKLKDVIPRQ
                     NFVIALQAAVGAKILARESVKAFRKDVTAKLYGGDITRKRKLLEKQKKGKKKMTNIGR
                     VEIPSSAFLAVLRK"
     gene            486..908
                     /locus_tag="VMQ48_02820"
     CDS             486..908
                     /locus_tag="VMQ48_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HUD08997.1"
                     /translation="MKRKNREKTNTSAKIFSGMFFLAGLAVLVLIGISLGKEVYRKRQ
                     IQKEIEGLQSQISQMGQQNGDMENLISYLSSTDFQEKQAREKLNLQKSDEKMIVLRKD
                     VVQPDSQPQNTPINPQAPPEDNSPNWQKWWKYFFAAKS"
     gene            918..1967
                     /locus_tag="VMQ48_02825"
     CDS             918..1967
                     /locus_tag="VMQ48_02825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUD08998.1"
                     /translation="MPKKEKLKKHKIWLIVGASAAVIFLFLLFLVLGIYKFNWSNRFF
                     VAATKVIPFPAVYVRGAGTIPVNEIKADDAAIKKFYESQDFEKIGMRVDFSTDQGKKR
                     LQVKEKEIINKLVENKVIESLAKARGINLTDSAVASELESNIQQFGNKDNLMSDLARL
                     YGWTLDDFEQKVVKPEMYSEKLTEIYASSLDTNSEQSKIKSLYDRVTGKKEDFAKVAK
                     ENSDGQSAANGGDLGWSTKDQLIQQVADKAFSMKVGDISEPVQSPLGFHVLKLLEKKT
                     ENSEDMVHLSQIFVKIPTYSDWLASQMKNYSVTVFLKDYQWNPSNGQVEFRDPAMKSF
                     EQSLPANSEGDPSVF"
     gene            complement(2044..2119)
                     /locus_tag="VMQ48_02830"
     tRNA            complement(2044..2119)
                     /locus_tag="VMQ48_02830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2084..2086),aa:Thr,seq:cgt)
     gene            complement(2131..2201)
                     /locus_tag="VMQ48_02835"
     tRNA            complement(2131..2201)
                     /locus_tag="VMQ48_02835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2167..2169),aa:Gly,seq:ccc)
     gene            2343..>2718
                     /locus_tag="VMQ48_02840"
     CDS             2343..>2718
                     /locus_tag="VMQ48_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HUD08999.1"
                     /translation="MPKKKKRIEELEKISNSENLWGDQEKAQKVMQELEGLRGEMKNL
                     SEYETEIDNLSEITSLAADDEKIKREIEKQIENIQKNIENIEFETLFQGEYDQSNAIL
                     SIHSGTGGTDAQDWAEMLLKMYL"
BASE COUNT          897 a          577 c          628 g          616 t
ORIGIN      
        1 acaaactggt aaaactcgac attctggttg ctggagacaa aatcgatgcc ttttccagaa
       61 ttgttccaga agagagagcg ttttccgagg gcaaagccac ggtggaaaaa ttgaaagacg
      121 tgattcctcg gcaaaacttt gtaatcgccc ttcaagcggc cgtcggcgca aaaatactcg
      181 ctcgagagag cgtcaaagcc ttccgaaaag atgtgacagc caagctctat ggcggggaca
      241 ttacccgcaa gcgaaaactg cttgaaaaac agaaaaaagg aaagaaaaaa atgaccaata
      301 tcgggcgggt cgaaatcccc agttcggctt ttttggcagt tttgcggaaa tagtggtata
      361 atataaacac tatgaaatcc cgacacaaga aaaaaatcag aataatttgg ctcatcatca
      421 ccattctggc cgttctttcg atggtggcct tcacaatcgc tccgctaatg agattctaag
      481 aaaaagtgaa aagaaaaaat cgcgaaaaaa caaacacatc tgccaagatt ttttcgggaa
      541 tgttttttct cgccggtttg gccgttttgg ttctcattgg aatctctctc ggcaaagaag
      601 tttatcgcaa gaggcagatc caaaaagaaa ttgaaggact ccagtcgcag atcagccaga
      661 tgggacagca aaacggagat atggagaatt tgatcagcta tctttcctcg accgattttc
      721 aggaaaagca ggcccgcgaa aaactcaatc tccagaaaag cgatgaaaaa atgattgttc
      781 ttcgaaaaga tgtcgtccag ccggacagcc agccgcagaa tactcccatc aatcctcaag
      841 ctccgccgga agacaattct ccgaactggc aaaaatggtg gaaatatttt ttcgcagcaa
      901 aaagctaata taattttatg ccaaaaaaag aaaagttgaa aaaacacaaa atttggttga
      961 ttgttggagc ctcagccgcg gtgatttttc tctttttgct atttttggtc cttggaatat
     1021 acaaattcaa ctggagcaac cgattttttg ttgccgcaac gaaagtcatt ccattcccgg
     1081 ccgtctatgt ccgcggagcc ggaaccattc ccgtgaacga gatcaaggcc gatgacgccg
     1141 ccatcaaaaa attctatgaa tcacaggatt ttgaaaaaat cggcatgcgg gttgattttt
     1201 ccaccgacca agggaaaaaa cggctgcaag tgaaagaaaa ggaaatcatc aacaaattgg
     1261 tcgaaaacaa agtgattgaa agcctggcca aagcgcgggg aatcaatttg acagacagtg
     1321 ccgttgcctc ggagttggaa agcaacatcc agcaatttgg aaacaaagac aacttgatga
     1381 gcgatcttgc gcgcctctat ggctggaccc tggatgattt cgagcagaaa gtcgtgaagc
     1441 cggaaatgta ttccgagaaa ctgactgaaa tatatgcgag cagcttggat accaactccg
     1501 agcaatccaa aataaagtca ctctacgacc gtgtgaccgg caaaaaagaa gattttgcaa
     1561 aagttgcaaa ggaaaattcg gacggccaaa gcgccgcaaa cggcggagac ctcggttggt
     1621 cgacaaagga tcaattgatc cagcaagtgg cggacaaggc cttctcaatg aaagtggggg
     1681 atatcagcga acccgtccaa agcccgcttg gttttcacgt cctgaaactc ctggagaaaa
     1741 agaccgaaaa cagtgaagat atggtgcatc tgtcccagat tttcgtcaaa attccgacct
     1801 attccgattg gcttgccagc caaatgaaaa attattctgt gacggttttt ctcaaggact
     1861 atcaatggaa tccgagcaac ggccaggtgg aattccgcga tcctgccatg aaatcttttg
     1921 agcaaagcct ccccgccaac tcggaagggg atccctcggt attctaacaa aacaaaatct
     1981 ccccctttta caaaggggga ttaaggggga ttttgacttt gcgataatgc aaagttttta
     2041 ttttggagcc gatgcgcggg atcgaaccgc ggacctacgc gttacgagtg cgtcgctctg
     2101 ccaactgagc tacatcggca ttcaattttg agcgggatac gggaatcgaa cccgtatttc
     2161 aaccttggga aggtcgtgtt ctgccactga actaatcccg cttttacttc aagaatataa
     2221 cagttttttt tgattctggc aatagaaaat cgcttatgct ataataaaat caatgcaaga
     2281 aatcatcgca aaaattgaag aactgcgcca aagaatcgcc tccgtaagcg actgtctttg
     2341 acttgcccaa aaagaaaaag aggatagaag agttggaaaa aatatccaat agcgaaaatt
     2401 tatggggcga tcaggaaaaa gcgcaaaaag tgatgcagga actcgaggga ttgcgaggag
     2461 aaatgaaaaa tttaagtgaa tatgagacag aaatcgataa cttgagtgaa ataaccagct
     2521 tggcggcaga tgatgaaaaa atcaagcggg agattgaaaa acaaatcgaa aatattcaga
     2581 aaaatattga aaatatcgaa tttgaaaccc tttttcaggg agaatacgac caaagcaacg
     2641 ccatattgtc gatccacagc ggaaccgggg gaacggacgc gcaagattgg gctgaaatgc
     2701 ttctcaagat gtatctcc
//
LOCUS       DATKGS010000114         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_80450, whole genome shotgun sequence.
ACCESSION   DATKGS010000114 DATKGS010000000
VERSION     DATKGS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80450"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VMQ48_02845"
     CDS             complement(<1..359)
                     /locus_tag="VMQ48_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HUD09000.1"
                     /translation="MKRKNREKTNTSTKIFSGMFFLAGLAVLVLIGISLGKEVYRKRQ
                     IQKEIEGLQTQIGQLGQQNGDMENLISYLSSTDFQEKQAREKLNLQKSDEKMIVLRKD
                     VVQPDSQPQNTPINPQAP"
     gene            complement(490..2277)
                     /gene="lepA"
                     /locus_tag="VMQ48_02850"
     CDS             complement(490..2277)
                     /gene="lepA"
                     /locus_tag="VMQ48_02850"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HUD09001.1"
                     /translation="MNNQQNIRNFCIIAHIDHGKSTLADRMLEITHTVEDRKMKEQLL
                     DTMDLERERGITIKLQPVRMKFKEYTLNLIDTPGHVDFQYEVSRSLAAVEGAVLLVDA
                     TKGVQAQTVANLYHAVEQGLEIIPVVNKIDLPNAEVEKSKKEIIHLLGCKEEEILEAS
                     GKTGEGVENILQKIVEKIPHPKGEQEKSLRALIFDSKYDTYKGVLAYVRIVDGKIKAE
                     ERILTMATKQNSSVIEVGYFKPNLEKTSMLSAGEIGYIATGFKTVEDCRVGDTIVLEK
                     SQAGAQALPGYKEIMPVVYASLYPKEGDDYNFMRDALGKLKLNDAAFSFEPEANLALG
                     RGFRCGFLGLLHMEIIQERLKREFEIDPVITTPSVVYQVSTKGKKEKTKIYSPLEMPD
                     PSVIESIEEPYVKLDLISPSKYLGSIMDLMRSTRAEYKNTEYIDAERLILTFEAPLTD
                     VIIDLHDKLKSASSGYASMNYEIIGNRRNLLVKLDILVAGDKIDAFSRIIPEEKAFSE
                     GKTTVEKLKDVIPRQNFVIALQAAVGAKILARESVKAFRKDVTAKLYGGDITRKRKLL
                     EKQKKGKKKMTNIGRVEIPSSAFLAVLRK"
     gene            complement(2434..>2636)
                     /locus_tag="VMQ48_02855"
     CDS             complement(2434..>2636)
                     /locus_tag="VMQ48_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09002.1"
                     /translation="PYINLDTFLGVFWQMTFAGVFGTATYFALAHLFNLEELHYLKKL
                     FSTRIFRLRPPLPEDPTEASGI"
BASE COUNT          619 a          595 c          598 g          824 t
ORIGIN      
        1 ggagcttgag gattgatggg agtattctgc ggctggctgt ccggctggac gacatctttt
       61 cgaagaacaa tcattttttc atcgcttttc tggagattga gtttttcacg ggcctgcttt
      121 tcctgaaaat cggtcgagga aagatagctg atcaagtttt ccatatctcc attctgctgt
      181 cccagctggc cgatttgcgt ttgaagcccc tcaatttctt tttggatttg ccgcttgcga
      241 taaacttctt tcccgagaga tattccaatc agaaccagaa cagccaaacc ggcgagaaaa
      301 aacattcccg aaaaaatctt ggtagacgtg tttgtttttt cgcgattttt tcttttcact
      361 tctttagaat ctcattagcg gagcaatcgt aaaagcaacc atcgaaagaa cagctaagat
      421 agtgatgata agccaaacta ttctgatttt tttcttgtgt cgtgatttca ttatgctgac
      481 attataccac tattttctca aaaccgccaa aaaggccgag ctggggattt caactcgtcc
      541 gatattggtc atttttttct ttcccttttt ctgtttttca agcagttttc gcttgcgggt
      601 gatgtctccg ccatagagtt tggccgtcac atctttccgg aaggccttga cggactcgcg
      661 ggcaagaatt tttgcaccga cagccgcttg cagggcgatg acaaagtttt gccgggggat
      721 gacatctttc aatttttcaa cggtcgtttt gccctcggaa aaggcttttt cttcgggaat
      781 aattctcgaa aaggcgtcga tcttgtctcc ggcaaccaaa atatccagtt tcacgagcaa
      841 attcctccgg ttgccgatga tttcatagtt catcgaggca tagccgcttg aagcgctttt
      901 caatttgtca tgaaggtcaa tgataacatc cgtgagaggc gcctcaaagg tcaggatcaa
      961 tctttccgcg tcaatatact ccgtgttttt gtattccgct cgggtgcttc gcatgagatc
     1021 catgatcgag ccgagatatt tcgaggggga aatcaaatcc agcttcacat acggctcttc
     1081 gattgattcg atgacagacg gatccggcat ttcaagaggt gaatatattt tggttttttc
     1141 ttttttgcct ttcgtactta cttgatagac cacgctgggg gtcgtgatca cgggatcaat
     1201 ttcaaactcc cgcttcagtc tctcctggat gatttccata tgcaaaagtc ccaaaaatcc
     1261 gcagcgaaaa ccgcgtccca gcgccagatt ggcttccggc tcgaaggaaa aagcggcatc
     1321 attgagcttc agttttccca gcgcatcgcg cataaagttg taatcatccc cttctttcgg
     1381 ataaagactg gcatagacga ccggcatgat ttctttgtat ccgggaagcg cttgagctcc
     1441 agcttggctc ttttccagaa cgatcgtatc accgacgcgg caatcttcga cggttttgaa
     1501 gccggtcgcg atgtagccta tttcgccggc agaaagcata cttgtttttt caagattggg
     1561 cttgaaatat ccgacttcaa ttacggagct gttctgcttg gtggccatgg tcaagattct
     1621 ttcttcggct tttattttcc catcgacgat tcggacataa gccagaacac ccttataagt
     1681 atcgtatttc gaatcgaaaa tgagcgcgcg aagcgatttt tcttgctcgc cttttgggtg
     1741 gggaattttt tcaacaatct tttggagaat attttccacg ccctcccctg tttttccgct
     1801 ggcttccaga atttcttctt ctttgcatcc aagaagatgg atgatttctt tttttgattt
     1861 ttcgacctcc gcgttcggaa gatcaatttt attgacgaca ggaattatct ccaatccctg
     1921 ttcaacggcg tgatagaggt tggcaaccgt ttgcgcttgc acgcccttag tcgcgtccac
     1981 cagcaaaacg gcgccctcga cggcggcgag agaccgggaa acttcatatt gaaaatccac
     2041 gtgtcccggc gtgtcgatca gattcagcgt atattctttg aatttcatcc gcaccggctg
     2101 caattttatc gtgattccgc gttcgcgctc gagatccatc gtatctaaaa gctgttcttt
     2161 catttttcgg tcttcgactg tatgcgtgat ttcaagcatt cgatcggcca gggtcgactt
     2221 tccatggtca atatgggcga ttatgcaaaa attccggata ttttgttggt tattcatatc
     2281 ctttaatcta gcctaattcg cggcaatttt caagtatttt tcaaaatgca agagtaggat
     2341 tgtatcctgc tccttttgtt tgcatttttg caaataaatg tttaaaaaag tattccgttt
     2401 agcgggacac aaaaattctt aattttaaat tttttagata ccgctcgctt ccgttgggtc
     2461 ttcggggagg ggcgggcgga ggcggaaaat gcgagtggag aaaagttttt tgaggtagtg
     2521 gagttcctcg agattgaaaa gatgggcaag ggcgaaataa gttgctgtgc cgaaaactcc
     2581 tgcgaaggtc atttgccaga atacgccgag gaatgtgtcc agattgatat atggtt
//
LOCUS       DATKGS010000115         5725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_113761, whole genome shotgun sequence.
ACCESSION   DATKGS010000115 DATKGS010000000
VERSION     DATKGS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113761"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VMQ48_02860"
     CDS             <1..1029
                     /locus_tag="VMQ48_02860"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HUD09003.1"
                     /translation="CPDHDTDCEIVKEESYLLKMETLQEELIEKIENDEFKIRPEKYK
                     KEILSFLTKEKLKDVSISRKNVKWGIPLPFDAEHTTYVWFDAFLSYLTGLGWDGEFLR
                     LNLKNKDLALKMDFWPADVQLIGKDILRVHATIWPIMLLHLGIELPKLLFTHGHLLSG
                     GRKMSKTIGNVIGVDEMLEKFGVDGTRYLLMSAGTFGEDVDVTMERLMEKYNADLANG
                     LGNLLSRVLRLAQNFQFQSPPKADQPRAEISNDSIFKNIENFEFEKALEKIWNIVRED
                     DKFVEENKPWELVKVNEDKFKEVMKKLISDLYLISELLVPFMPDTAGKIKKALETKEP
                     TILFPRIK"
     gene            1084..1878
                     /locus_tag="VMQ48_02865"
     CDS             1084..1878
                     /locus_tag="VMQ48_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUD09004.1"
                     /translation="MIDTHAHLDFENFEEDREEVISRAFDNGVKKIINIGVDLETSKK
                     SIALAEQHENIFAAVGFHPHDAEEILNSPSDDDFEELKKLAKHKKVVAIGEIGLDYSR
                     FGDTHQIEKQKELLLKQLKMASELNLPVVIHCREAWEDLYEIISNFKFQISNFQLHCY
                     SGGTEDTEKFLKLPNVYFSFSGNITYPKPANRAEKLAEAVRMIPLDRIMLDSDSPFLA
                     PQAMRGKRNEPAYIRYIVEKIAEIKGISGAEVERITDLNAIKFFGI"
     gene            2042..3589
                     /locus_tag="VMQ48_02870"
     CDS             2042..3589
                     /locus_tag="VMQ48_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUD09005.1"
                     /translation="MNSSGAPLIKLEKLNIVFNVGKPNEVRVLDNLDLDIYPHEYLIL
                     FGPSGCGKSTLLNVISGLLPPTSGKVEVEGKEITTFTEKEKLEFHRRRIGMIFQSFFL
                     VPTLSIIDNVCLPRSFAGDDSPASRQDAMNFLKRFGIESQAEKFPNDLSGGQRQRVAI
                     ARALMNSPSIIVADEPVGNLDSKATYNVLSILKDLNEIDKKLVVLVTHDPSHLSYGDR
                     VIHMQDGKITKIVEVLEKKEPTEKGEQEDLEEREKIGKEWDSFKEGTLGKAWELHKKG
                     QLDKDWELVKKEELGKAWNSFKEGKAGENWEIGKEYELVRKEDLTERRKEHIAPELLT
                     LMRSFKNFTPDQVGTLLIPFKAQQIFSHIFFHIPETQLDLAKKLFQDFIAGGIPLEDF
                     EKRLVEEEDKGGAGWDHTKAKKLTKGIEQIFEQVKKIDFGNSEKSAQELGEYLIENFH
                     LRLGEMEKIKFLEYVKMRLENELTMDELLDLVDRPVVKGGVGLDRRMAIKVAREMEII
                     MLARYSM"
     gene            3591..4781
                     /locus_tag="VMQ48_02875"
     CDS             3591..4781
                     /locus_tag="VMQ48_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUD09006.1"
                     /translation="MTTKDLLKLSTRIFTARKGRTALTVLGMGIGFGAILFLVSLGYG
                     LQGALLQNITSTGALTTLDVAPNEQDGKFITSSAVDEMKKINGVQGVETSYNFNSRIK
                     LGDTGSDARGEIASFGFLDLEGLKVTVGKPLDKNNSKEILVSSAIGKVFGKDPKDLVG
                     QKISFSLLPSATTEVPAPRPNIEAGDYTIAGVTENEEAVFYAPTDGLNIPADIPFAKA
                     KVECNSSNAMTSVKDTISQKGFAVSSLSDTVSQVNKLFSVVNVILGLFGIITLAVSAI
                     GMFNTMTVALLERTTEIGIMRSIGASRKDILFMFIIESTLMGFCGGIAGIILGIASGQ
                     IVNLIVNIAAKYMGGKSLNLFAYPLWFLGFVLAFSIIIGFATGIGPAKRASSLDPLEA
                     LRSR"
     gene            4888..5520
                     /locus_tag="VMQ48_02880"
     CDS             4888..5520
                     /locus_tag="VMQ48_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HUD09007.1"
                     /translation="MILKKTIKYFSLAILLVFLGYFSMKVLSLYSARNRTNQEVATAR
                     NETTGGKVLGEEGNQVPTQMYASQNFRTPQITFGGDAITMPSGQQAVAPEILDMRSEL
                     LTTKTDQQVKFLLTWKTNKLCNSSIDYTKEGQAEAKTVSEEGYGFVHSVEIAPLNYST
                     SYSYAVTARDKWGTEIKSDKLAFYTGAQNVSVFDLLAGAFKDMFGWAGGK"
BASE COUNT         1755 a         1143 c         1386 g         1441 t
ORIGIN      
        1 tgtccggacc atgatacgga ttgcgagata gtgaaagaag aaagctatct tctgaaaatg
       61 gaaactttgc aggaagagct gatcgagaag atagaaaacg acgagttcaa gatccggccg
      121 gaaaaataca aaaaagaaat tttgtcgttt ctcacgaaag aaaagctgaa ggatgtttcc
      181 atctcgcgca agaacgtaaa atgggggatt cctttgccgt ttgatgcgga gcatacgacc
      241 tatgtatggt ttgatgcctt tttgagctat ctcaccggtt tgggctggga tggagaattt
      301 ttaaggctaa accttaaaaa taaagattta gccttaaaaa tggatttctg gccggcggat
      361 gtgcaactga tcggaaaaga tatcctgcgg gttcacgcga cgatctggcc gataatgctc
      421 ttgcacctcg ggatcgaact gccaaaactt ttattcaccc atgggcacct gctttccgga
      481 gggaggaaaa tgagcaaaac cattggaaat gtcattggag ttgatgaaat gctggaaaaa
      541 ttcggtgtgg atggcacgcg ctatctattg atgtcggctg gaacattcgg ggaagatgtc
      601 gatgtgacaa tggagcgtct catggaaaaa tataacgcgg atttggcaaa cgggttggga
      661 aaccttctat cgcgagtctt gcgactcgct caaaattttc aatttcaatc tccgcccaag
      721 gcggatcagc ctcgggctga aatttccaat gactcaattt tcaaaaatat tgaaaacttt
      781 gaattcgaaa aagcactgga aaaaatttgg aatatcgtaa gagaagatga taaattcgtg
      841 gaagagaaca agccctggga gcttgttaaa gtcaatgaag ataaattcaa agaagtgatg
      901 aagaagttaa tttctgatct ttatttaatt tcagaattac tcgtgccgtt tatgccagat
      961 actgcaggga aaataaaaaa agcgctagag acgaaagagc cgacaatttt gtttccgcgg
     1021 ataaaataac ttttaatttt gcattgtaat ttttcacttt gcatttttca cttttaactt
     1081 cctatgatcg acacgcacgc ccatttggat tttgaaaatt tcgaggaaga ccgcgaggaa
     1141 gtgatttcgc gggcttttga taatggggtg aaaaaaataa tcaatatcgg agttgacctc
     1201 gagacgagca aaaagagcat cgcgctggca gagcagcacg aaaatatatt cgcagcagtc
     1261 ggttttcatc cacacgacgc agaagaaatt ttgaattcac ctagcgacga tgattttgaa
     1321 gagttaaaaa agctggcaaa gcataaaaaa gtcgtggcta ttggagaaat tggattggat
     1381 tattcccgtt ttggggatac gcatcaaatt gaaaagcaaa aagaattgct tctcaaacaa
     1441 ttgaaaatgg ccagcgaatt gaatttgcct gtcgtgatcc attgccggga agcgtgggag
     1501 gatctatacg aaataatttc aaatttcaaa tttcaaattt caaattttca gctgcattgc
     1561 tattcgggcg ggacagaaga tacggaaaag ttcttgaaat taccaaatgt atacttttct
     1621 ttttcgggaa atatcactta tccaaaaccg gcgaataggg cggaaaagct ggccgaggcg
     1681 gtacggatga ttccccttga caggattatg ctggacagtg atagtccttt tctcgcgcca
     1741 caggcgatgc gaggaaaaag aaatgagcca gcgtatatcc gatatatcgt cgaaaaaatc
     1801 gccgaaataa aaggaatatc gggagcggaa gtggagagaa ttactgatct taacgcaatc
     1861 aaattttttg gaatttagcc tgttaagcaa ggaactttct ctacgaacta ctaatcaatt
     1921 acgaatatac gaaatatata caaatatctt ttctcttgaa tacacgaacc acttatttgc
     1981 acgaattagt atattcgtac aaaattcgta gttcgtagag acttcgtgat atactataaa
     2041 catgaattct tcaggtgcgc cgctcatcaa gctggaaaaa ctgaatatcg ttttcaacgt
     2101 gggaaagccc aatgaggtga gagttttgga taatcttgat ctggatatat atccgcatga
     2161 gtatctcatt ctctttggtc cttcgggttg cgggaaatcg accctgctca acgtgatctc
     2221 gggactcctc cctccgacga gcgggaaagt cgaagtcgaa ggcaaggaga tcacgacttt
     2281 caccgagaag gaaaaattgg aattccaccg ccgccggatc gggatgatct ttcagtcttt
     2341 ttttcttgtt cccactcttt cgatcatcga caatgtctgc ctgccgagat ctttcgcggg
     2401 cgacgattct ccggccagcc ggcaagatgc aatgaatttt ctcaaacggt tcgggatcga
     2461 gtcccaggcg gaaaaatttc caaacgatct ttccggcggg cagaggcaaa gagtggcgat
     2521 cgcgagagcc ttgatgaaca gcccgtccat cattgtcgcg gacgaaccgg tgggaaacct
     2581 tgattccaag gcgacctaca acgttctttc aatcctcaag gatctcaatg agatcgacaa
     2641 aaagcttgtc gttcttgtca cgcacgatcc gagccatctc tcctacggcg atcgcgtgat
     2701 ccatatgcaa gacggaaaga tcacgaagat tgtcgaagtg cttgagaaaa aagagccgac
     2761 ggaaaaagga gaacaggaag atcttgaaga gcgggaaaag attggaaaag aatgggactc
     2821 tttcaaagaa gggacgcttg ggaaggcctg ggaacttcac aaaaaagggc aactcgacaa
     2881 agattgggaa ttggtcaaaa aagaagaact tggaaaagcc tggaattctt tcaaagaagg
     2941 gaaggcggga gagaattggg aaattggcaa agaatacgaa ttggtgagga aagaagatct
     3001 caccgagcgc cgcaaggagc atatcgctcc ggagctgctc accctgatga gatctttcaa
     3061 aaatttcacg cccgaccaag tcgggacgct tttaatcccc ttcaaggcac agcagatttt
     3121 ttcccatata ttttttcaca ttcccgaaac acaacttgat ttggcgaaaa aactgttcca
     3181 ggattttatc gcgggaggaa tacccctcga agactttgaa aaaagactgg tcgaagaaga
     3241 ggacaaagga ggcgccgggt gggaccatac caaagccaaa aagctcacaa aaggaatcga
     3301 gcaaattttc gaacaggtga aaaaaataga tttcggaaat tcagaaaaat cagcgcaaga
     3361 actgggtgaa tatttgattg aaaattttca cttgagactc ggagaaatgg aaaaaataaa
     3421 gtttttggaa tacgtcaaaa tgcgcctcga aaacgaactc acgatggacg agctgctcga
     3481 tcttgtcgac cggccggtgg tgaagggcgg agtcggactc gaccgccgga tggcgatcaa
     3541 ggtggctaga gagatggaaa taatcatgct ggcccgttat tcaatgtaaa atgactacca
     3601 aagatttgct aaaactttcc acccgaattt tcaccgcgcg gaaagggcgg acggcgctca
     3661 cggttttggg tatgggaatc ggattcggag caattctgtt tttggtttct ttgggatacg
     3721 ggctccaggg agctttgctt caaaatatca cttcaactgg agcgctcacg acacttgatg
     3781 tcgcgcccaa tgagcaggac ggaaaattca tcacctcttc tgcggttgat gagatgaaaa
     3841 aaataaatgg agtgcagggg gttgaaacaa gttataattt caactcaaga atcaagctgg
     3901 gcgacactgg gtctgacgcg cgcggagaaa tcgcgagttt cggatttctt gatttggagg
     3961 gtctgaaagt cacggtcgga aaaccgctcg acaaaaataa ttcaaaagaa attctggttt
     4021 cttcggctat cggaaaagtt tttgggaaag acccgaagga tctcgtcgga caaaaaattt
     4081 ctttttcttt gcttccttcg gcgactacgg aggttcctgc gcctcgcccg aatattgagg
     4141 cgggggacta cacaattgcg ggtgtcacgg aaaacgaaga ggctgttttt tatgcaccaa
     4201 cggatggcct caatattccc gcggatatcc cgtttgctaa ggcgaaagtg gaatgcaatt
     4261 caagtaatgc tatgacttcg gtcaaagaca cgatttccca aaaaggtttt gctgtttcat
     4321 cgctttcaga tactgtttcc caagtgaaca agcttttcag cgtcgtgaac gtcatccttg
     4381 gccttttcgg cattatcacg ctcgcagtca gtgcgatcgg gatgttcaac acgatgacgg
     4441 tcgccctact cgaacggacc acagaaatcg gaattatgcg ctcaattggc gcgtcgagaa
     4501 aggacattct tttcatgttc atcatcgaat cgacgctgat gggattttgc ggcggtattg
     4561 cgggcatcat tctgggcatc gcatcggggc agatcgtcaa cttgatcgtg aatatcgcgg
     4621 cgaaatatat gggcggaaaa tccctcaatc tctttgccta tccgctttgg tttttgggat
     4681 tcgtcctggc cttttcaatt atcattggat ttgcgaccgg catcggaccg gccaagcggg
     4741 cgagttcgct tgacccgctg gaagcgctga gatcgagata attttataat ttttaaacaa
     4801 tgtctaaatt ttttaatgtt taaaaattga gacattaaga tttatttaaa aattaaaaat
     4861 tggaaattaa aaatttagtc ataaaatatg atactcaaga aaacaatcaa atacttttcc
     4921 ctcgccattc tccttgtctt tctcggatat ttttcaatga aagttctttc attgtattcg
     4981 gcgaggaaca gaacaaatca agaagtggcg actgcgcgaa acgaaacaac aggcggaaaa
     5041 gttcttgggg aagaggggaa tcaagtgccg acgcaaatgt acgcgagcca gaatttccgc
     5101 acgccgcaaa tcacattcgg cggggacgcg atcacaatgc cgtcaggcca gcaagcagtt
     5161 gctccggaaa ttctggatat gcgctcggag cttctcacga caaaaaccga ccagcaagtg
     5221 aaatttctct tgacgtggaa aacgaacaag ctttgcaatt catcaatcga ttatacgaag
     5281 gaagggcaag cagaagcgaa aacggtttcc gaagaaggat atggtttcgt ccatagcgtc
     5341 gagattgcgc cgctcaacta ttcaacttcg tattcatacg cggtgacggc gcgcgacaaa
     5401 tggggaaccg aaatcaaatc ggacaagctg gctttctata ccggcgcgca gaatgtttcc
     5461 gtctttgatc ttcttgccgg cgcgttcaaa gatatgttcg gttgggcggg gggaaagtaa
     5521 ctgtggataa ctaaaaaagt cggggcaaaa aaaatacccg acttgacaaa agtttttgag
     5581 attgaataga aaataatttt ataaaaaata ttattttagc gaaggatcaa aaaaaataga
     5641 aaaacaacca atccgatttt tggcgtaaaa ccaaaaaccg atatttccga catttagccg
     5701 agctaaaggg aaatatcggt ttttt
//
LOCUS       DATKGS010000116         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_21355, whole genome shotgun sequence.
ACCESSION   DATKGS010000116 DATKGS010000000
VERSION     DATKGS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21355"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VMQ48_02885"
     CDS             complement(<1..824)
                     /locus_tag="VMQ48_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HUD09008.1"
                     /translation="MTANELRQKYLDFFKEKGHAIIPSAPLVPENDPTVLFTTAGMHP
                     LVPFLMGEKHPQGKRLANIQKCVRTGDIDDVGDNRHDTFFEMLGNWSLGDYFKKEAIE
                     WSFEFITGEKWLGLDPRRLYVTVFEGDDDAPLDQESINIWQEQFKKAGIEAEIAGSDK
                     SVSENHRIIPLGKDDNWWGPAGETGPCGPCTEMFYDVSPENGSLTGNFENLVNNFRLL
                     EFWNDVFMEYNKKEDGTFERMAQQNVDTGMGLERVAVIVGGKDNIFDTELFQPIIR"
     gene            complement(833..1147)
                     /locus_tag="VMQ48_02890"
     CDS             complement(833..1147)
                     /locus_tag="VMQ48_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09009.1"
                     /translation="MGLLDDYQNIDETADDGSPVLDPTTLRRKRLDIERQIVILDSDF
                     RKTQREIEQYEMQKRKFKKEEERIRIDREDLDKKLKNLDNDRVSLEDQIRLLKKKLKI
                     LQ"
     gene            complement(1190..>2211)
                     /gene="mnmA"
                     /locus_tag="VMQ48_02895"
     CDS             complement(1190..>2211)
                     /gene="mnmA"
                     /locus_tag="VMQ48_02895"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HUD09010.1"
                     /translation="FGVHFRFFDDASAKKSLNKAKKVAKTLEIPLEIVDARKEFKKKI
                     IDYFIKSYKKGITPNPCVACNREMKFALLKELMKKNKADYIATGHYARIKREFSIFNF
                     QFSNNSKISKFQNVKLLQATDKSKDQSYFLYRLSQNNLSRIIFPLGTLKKTEVRKLAK
                     KFKLPIFGEKESQDVCFLAGTDINSFLKKYLKPKSGNIVDESGKILAKHKGLPFYTIG
                     QRKGIEIGGGGPYFVIGKNLKKKELAVSNDPTKLLVKKFEVNQVNWANKGNWGNKGDR
                     LGKYILQAHVQIRYHSEKIPAIIRLSKSGKLVIETKKPLRAVTPGQSTVFYKKGEVLG
                     GGVIC"
BASE COUNT          568 a          522 c          383 g          738 t
ORIGIN      
        1 tttctgataa tcggttgaaa aagttccgta tcaaaaatat tatctttgcc gcccacaatc
       61 accgccactc gctccagacc cattcctgta tccacatttt gttgcgccat tcgctcaaaa
      121 gttccgtcct ctttcttgtt atattccata aaaacatcgt tccaaaattc aagcaggcga
      181 aaattattta ctaaattttc aaaatttccg gtgagtgatc cattttccgg tgaaacatcg
      241 tagaacattt ccgtgcacgg tccgcagggt cccgtttctc cggccggccc ccaccagttg
      301 tcgtctttcc cgagaggaat aatccgatga ttttcgctta cgcttttatc cgatcctgcg
      361 atctctgcct ctatccccgc ttttttgaat tgttcttgcc agatattgat cgactcctga
      421 tcaagcggcg cgtcatcatc accttcgaaa acagtcacat agagtcggcg cggatccagt
      481 ccaagccatt tttctccagt aataaattca aaactccact caatcgcttc ttttttgaag
      541 tagtctccaa gtgaccagtt tccaagcatt tcaaaaaaag tatcatggcg attatccccc
      601 acatcatcaa tatcgcctgt gcgcacgcat ttttggatgt tagctaatct ttttccttga
      661 ggatgttttt cacccatcaa aaacggcacc agcggatgca tcccggcagt cgtgaaaagc
      721 acggtcggat cattttccgg caccaaaggc gccgacggaa tgatcgcatg ccccttttcc
      781 ttgaaaaaat cgagatattt ttgacgaagt tcgtttgcag tcataaaaaa tattattgta
      841 atattttcag cttctttttg agcagtcgaa tctgatcctc caaagacacc cgatcattgt
      901 ccaggttttt gagctttttg tcaagatctt cccggtcaat gcggatgcgt tcttcttctt
      961 tcttgaattt ccttttctgc atttcatatt gctcgatttc tctttgggtt tttctgaaat
     1021 ctgaatccaa aatgacgatt tggcgctcga tatccaagcg ctttcggcgc aacgtagtcg
     1081 gatcaagcac cggagatcca tcgtccgctg tttcatcgat attttgatag tcgtcaagca
     1141 atcccataat tttaaaattt tcaatgactc aatttctaaa ttaaaaaaat tagcaaatta
     1201 caccaccacc tagaacctcg ccttttttat agaaaaccgt cgattgcccc ggtgtcaccg
     1261 cccgcaaagg ttttttggtt tcaatcacca atttccccga cttgcttaat ctgattatag
     1321 caggaatttt ttccgaatga tagcggatct gaacgtgcgc ctgaaggata tattttccga
     1381 gccgatcccc cttgtttccc caatttccct tattcgccca attcacctga ttgacttcaa
     1441 actttttcac aagcagtttt gtcggatcat ttgaaacagc cagttctttt tttttcaaat
     1501 tttttccaat cacaaaatac ggtccgcctc ccccaatttc gattcctttg cgctggccga
     1561 tggtataaaa tggcaaccct ttgtgttttg caagaatttt tccagattca tcgacaatat
     1621 ttcccgactt aggcttcaga tattttttca aaaacgaatt tatatccgta ccggccaaaa
     1681 aacaaacatc ttgcgattct ttttctccaa aaatgggtag cttgaatttt ttcgccaatt
     1741 tcctcacttc ggtttttttg agcgtgccga gagggaaaat gattctggat aagttgttct
     1801 gactcaggcg ataaaggaag tatgactggt cttttgattt atctgtggct tgcagtaact
     1861 taacattttg aaatttagaa attttagaat tatttgaaaa ttgaaaattg aaaattgaaa
     1921 attcccgctt tatgcgggca tagtgcccag tagcgatgta atccgcttta ttctttttca
     1981 tcaattcttt cagcaaagca aatttcatct cccggttgca agccacacac ggattgggcg
     2041 tgatgccttt tttgtatgat ttgatgaaat aatcaattat ttttttcttg aattcttttc
     2101 gcgcatcaac tatttccagt ggaatttcaa gtgtttttgc gacttttttt gctttattca
     2161 aggacttttt ggcgcttgcg tcatcgaaaa aacgaaagtg caccccaaaa a
//
LOCUS       DATKGS010000117         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_157047, whole genome shotgun sequence.
ACCESSION   DATKGS010000117 DATKGS010000000
VERSION     DATKGS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157047"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VMQ48_02900"
     CDS             complement(<1..517)
                     /locus_tag="VMQ48_02900"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HUD09011.1"
                     /translation="MDQTKLLKKVAKVLEQLNIPYIVTGGVAVTVWGRPRFTADIDIA
                     IEISLSKSKDLLKALQKIDRKIYISEVAVNQAVMGNGEFNLIHPDGLKVDFWVLKNDI
                     YARSRIKRRKLKKAWGQNISFISPDDLILSKLIWYKKTGSTRQLEDIESIIAIQKKLD
                     WKYIRRWAEIHA"
     gene            complement(677..994)
                     /locus_tag="VMQ48_02905"
     CDS             complement(677..994)
                     /locus_tag="VMQ48_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HUD09012.1"
                     /translation="MHLIIPMAEEKKEDKDKKLTKAEEQKLHVYFVDKVITLMTGAMG
                     LVAALAWNDAMRKLFERVFGTAGSGDVTAMFIYAAIVTATVVIVTYRLTRFSERMKKK
                     NEK"
     gene            1116..1188
                     /locus_tag="VMQ48_02910"
     tRNA            1116..1188
                     /locus_tag="VMQ48_02910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1149..1151,aa:Ile,seq:cat)
     gene            1204..1276
                     /locus_tag="VMQ48_02915"
     tRNA            1204..1276
                     /locus_tag="VMQ48_02915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1237..1239,aa:Val,seq:gac)
     gene            complement(1329..2300)
                     /locus_tag="VMQ48_02920"
     CDS             complement(1329..2300)
                     /locus_tag="VMQ48_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09013.1"
                     /translation="MIATNNSPQKKSVPYIEILKQAGRIVWQKRFLLWFGLLMALGSP
                     GSFNIGNNNSFGSQNDAAKSFFETHWGIVLALVIVLFIIGIILLLISLVAKAGLVKSV
                     NLVAQNKKTDFKTGWKSGKKYFGKLFKLAILLFLAILAVVIILAVPVIYLVAIKFWLG
                     AILVGIFAIAIFIPLIFIFSLTKIYAEFYIILSDLSLRSAIEAGYNLLLKNIGHSIVF
                     GLLLIAVSIVAGIVLLPVAGIALVILIPTGIAFFYLSKIAFAIFLGFAILFFLAAILF
                     VSSIFQTYKTAAWTLFFQEIAKVEKPEAEKIAETELEESLAATPEKV"
     gene            complement(2284..>3156)
                     /locus_tag="VMQ48_02925"
     CDS             complement(2284..>3156)
                     /locus_tag="VMQ48_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HUD09014.1"
                     /translation="GPGRFDRRVVMDLPDIREREAILKIHTKNMPVAKTVDFKKIAAR
                     TAGFSGADISNLVNEAAILAARRGKKIVEDDELRVSIEKVILGPERKSKILNVAEKEI
                     TAYHEAGHALIASNLKNADPVQKVSVISRGQAAGYTLNVPQEDKRLYTRAYFIDELAV
                     FLGGYVSEKIFFGDVTTGASNDLERATDTARKLVTRYGMSSLGPRTFGHKEELVFLGK
                     EMSEERNYSEATAKEIDMEVSRFISDAYKTAEKIITEKKTTLERIAKTLLEKETLEQE
                     EFNELLKEKNDSDK"
BASE COUNT          786 a          703 c          750 g          917 t
ORIGIN      
        1 tcgcatgaat ttccgcccag cgcctgatgt atttccagtc cagttttttc tgaatcgcga
       61 tgattgattc gatatcttcc agctgacggg ttgagccggt ttttttgtac caaatcaatt
      121 ttgataagat caaatcatcg ggagagataa acgaaatatt ttgcccccaa gcttttttca
      181 actttctgcg ctttatccgg gatcgagcgt agatatcatt tttcagaacc caaaaatcca
      241 ctttcagccc gtcgggatgg atgagattga attccccgtt tcccatcacc gcttggttga
      301 cggcgacttc actgatataa atttttctgt cgattttttg cagggctttc aaaagatctt
      361 ttgattttga aagtgaaatc tcaatggcta tgtcgatatc ggccgtgaag cgcggtctgc
      421 cccaaaccgt gacagcgacc ccgccggtga cgatgtacgg gatattcagc tgctcaagaa
      481 ctttggcaac ttttttgaga agttttgtct gatccacatt tttttcctaa tgaatttaat
      541 tctcttgcaa agcgttgaaa gatgtccaac atttccagtt ttttctccac gggcatttcg
      601 cgaaaaatac cgtcctggat ttcctgtgcc gatttgttct tatccgatga tttattcatg
      661 ataaaaatat attattctat ttctcgtttt tctttttcat tctttccgaa aatcgggtga
      721 ggcggtaggt gacgatgaca acagttgcgg tgacgatggc agcatagatg aacatggccg
      781 tgacgtcgcc tgatccggcc gtgccgaaaa cgcgctcgaa aagttttcgc atcgcgtcgt
      841 tccaagcgag cgcggcgacc agtcccattg cgccggtcat cagcgtgatg actttgtcga
      901 caaaatatac gtggagtttt tgctcctcgg ctttggtcag ttttttgtct ttatcctctt
      961 ttttttcttc ggccataggg atgatcagat gcatttatat tgtaaattta gcatttaacg
     1021 cgataagtgt ccattttctc tatacgctat aaacagaata aactgtcatt gactatatca
     1081 atatatttct ttaggattat aatgttatga gcgacgggac cgtagctcag cggttagagc
     1141 ggctggctca taaccagttg gtcataggtt cgaatcctat cggtcccaaa ataacaatat
     1201 tacgggcaat tggctcagtg gttagagcgc ttccttgaca tggaagaggt cacaggttcg
     1261 attcctgtat tgcccacaaa aaatctccgc catcaaagcg gagattttag tttgtacgaa
     1321 aatgactcct acactttttc cggtgtggca gcgagggatt cttcgagctc agtctctgct
     1381 attttttccg cctccggttt ttcaaccttg gcgatttcct ggaaaaacaa agtccaagcg
     1441 gcggttttgt aggtttggaa aatagaagag acgaacaaaa tagcggccag gaaaaacagg
     1501 atggcaaagc cgaggaagat cgcaaacgcg attttgctca aatagaagaa tgcgattcct
     1561 gtcgggatca aaatgacaag ggcgattccg gcgacgggca ggagaacaat tccagctacg
     1621 atactcacgg cgatgaggag gaggccgaaa acgattgagt ggccgatatt tttgaggagc
     1681 aaattatacc ccgcttcgat ggcgctgcgg aggctcaaat cggaaaggat gatatagaat
     1741 tcagcataaa tttttgtgag cgagaagatg aaaataagcg ggatgaaaat agcaatggcg
     1801 aaaatcccaa ccaaaattgc gccaagccaa aactttatgg cgacaagata gatcacggga
     1861 acggccagga tgatgacgac cgcgagtatt gccagaaaaa gcaagatggc gagtttgaaa
     1921 agttttccga agtatttttt tcctgatttc cagccagtct tgaaatccgt ttttttgttt
     1981 tgagcgacga gattcacgga tttcacgagt ccggctttgg cgacaagaga gatgagaagc
     2041 aaaattattc caataatgaa gagaacaatg acaagcgcca ggacaattcc ccagtgtgtt
     2101 tcaaaaaagc ttttagcggc gtcattttgg gaaccaaagc tgttgttgtt tccgatattg
     2161 aagctgccgg gcgatccaag agccatcaag agcccaaacc aaagcaagaa acgcttctgc
     2221 cagacaatcc ggccggcttg tttcagaatt tcaatatagg gaacagattt tttttgcggg
     2281 gaattatttg tcgctatcat ttttttcttt tagcagctca ttgaattctt cctgctcgag
     2341 agtttctttt tcgagaaggg ttttggcaat tctttcgaga gttgtttttt tctcggtgat
     2401 gattttttct gcggttttgt aggcgtccga aatgaagcgg gacacctcca tgtcgatctc
     2461 tttggcggtc gcttcggaat aatttctctc ttcactcatc tcttttccaa ggaacacgag
     2521 ttcttctttg tggccgaaag tccgcggtcc gagagagctc atcccatatc tcgtcacgag
     2581 ttttcgcgcc gtgtcagtcg cgcgctcaag gtcattcgat gcgccggtcg tcacgtcgcc
     2641 aaagaatatt ttctcgctca cgtatccgcc gagaaacacc gccagttcgt cgatgaaata
     2701 ggcacgggta tagagccgct tgtcttcttg tgggacattc aaggtgtagc cggcggcttg
     2761 gccgcgcgag atgacggaaa ctttttgcac cggatcggcg ttttttaggt tcgaggcgat
     2821 gagcgcatgt ccggcttcat gataggcggt gatttctttc tccgccacat tcagaatctt
     2881 gctttttcgt tcaggaccaa gaataacttt ttcaatggaa acccggagct catcatcttc
     2941 gacgattttt ttcccgcgcc gggcggccaa aatggccgct tcattcacga gattggaaat
     3001 atcggctccg gaaaaaccgg ctgttcgggc ggcaattttt ttgaaatcaa ccgtcttggc
     3061 cacgggcata tttttcgtgt gaattttgag aatggcttcc cgttcccgga tgtcgggaag
     3121 gtccataacc acccgccggt cgaatcttcc cggccc
//
LOCUS       DATKGS010000118         3586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_55446, whole genome shotgun sequence.
ACCESSION   DATKGS010000118 DATKGS010000000
VERSION     DATKGS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55446"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VMQ48_02930"
     CDS             <1..298
                     /locus_tag="VMQ48_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09015.1"
                     /translation="GVGFLVLIFSVIYDFHNRRFGIGNNFNKIQLFFFGHLKSLNRGN
                     NTNHLASVVDQPNRGYSDVFIDPNARPAKFLRTAVKKSSSYGFLFKNYCGDDGN"
     gene            complement(277..675)
                     /gene="ssrA"
                     /locus_tag="VMQ48_02935"
     tmRNA           complement(277..675)
                     /gene="ssrA"
                     /locus_tag="VMQ48_02935"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(836..1279)
                     /gene="smpB"
                     /locus_tag="VMQ48_02940"
     CDS             complement(836..1279)
                     /gene="smpB"
                     /locus_tag="VMQ48_02940"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HUD09016.1"
                     /translation="MSVLAVNKRASFDYDISERFEAGIVLRGYEVKAVKTGHISLKGS
                     FVTVRGEELFLTNANIPLYKHAGAIPSYEPTRPRKLMLHRSEIASLIGKSRVEGLTLV
                     PIQVYTKRGLLKLEFGIGKGRKKFDKRDVIQKRESDRKIERALKR"
     gene            1338..3038
                     /gene="argS"
                     /locus_tag="VMQ48_02945"
     CDS             1338..3038
                     /gene="argS"
                     /locus_tag="VMQ48_02945"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HUD09017.1"
                     /translation="MKTEIQQLIQQAIKSAFGMDFDVEKIKVDYPPEGMGDFSTNVAL
                     LLAKEIGKSPMEVAEELRDKIALKDVGLEKVEVALPGFLNLYLAEKAVQEVIAEINEK
                     GSQFGNLEKKNEKIMIEYSQPNTHKEFHIGHLRNVFIGSTLVNVLKKAGSDVIAANYI
                     GDTGTHIAKCLWGLVKFHPGEDLSKIENKTEFLGKVYSEAVQKIESDEKYKDEFKQIQ
                     KRLEDGDEEIVKLWKETRQWSLDEFSAIYKELGVSFDEIFYESEEEIAGKKMLPNLLE
                     KGVAKKSQGAVIADLEEYDLGVLVLQRGDGSVLYGLKDIPLAIEKFEKYKIDGSVIVA
                     DVRQSLYFRQIFKILEQMGFEKKMVHIGYEFVALKGGESMSSRKGNVVPAQYLIAAVA
                     NEVKRKFPETDISSEIGLGAVKFFMLKYSSGTKIDFDINESVKIEGTTGPYVQYAHAR
                     IASILQKASLLRLSLSGGSLRLSLSNVSNKRERALIFELLKFPDLIEEISRNFEVHHL
                     SKYATDLADKFHSFYNDCKVIDETNPELTAARLELVKAVQIVLAEVLRLMGISAPDKM
                     "
     gene            3103..>3586
                     /locus_tag="VMQ48_02950"
     CDS             3103..>3586
                     /locus_tag="VMQ48_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HUD09018.1"
                     /translation="MFLDIETLPAPEHMHEVLKEIHAYKKKKKTRNGEKYATSFESFL
                     AGTGLDGTFGRILCISYAINDDMIHCICDEDEKKVLENFWEAAKDVDLFVGFNIIEFD
                     MKYLVQRSIVHGVKPKTKMRLSFAKYRSEPMYDVMHEWNQWSFGKGVGLDTLARALGF
                     P"
BASE COUNT         1083 a          677 c          841 g          985 t
ORIGIN      
        1 gggggttggc tttttggtac tgatattttc cgtaatctat gattttcaca accggcggtt
       61 tggcattggg aacaatttca acaagatcca actctttttc tttggccatt tgaagagcct
      121 caaccgtggg aacaacacca accatttggc cagtgtcgtc gatcagccga acaggggata
      181 ttctgatgtt ttcattgatc cgaacgcgcg gcccgcgaaa ttcctgcgga cggcggtgaa
      241 aaaatcttct tcttatgggt ttttatttaa gaattactgt ggagatgatg ggaattgaac
      301 ccatgtccaa atttattcct caaaaaatat tttacaagct tagtttattt gtttgttgaa
      361 gttttttcaa gtagaaaata aacaaaacct tgaaaaactt tctttgttca atggtcattg
      421 atagccgcaa agaatgctat caatcaagcc caatgtatga caccggatac tgcctattgg
      481 gcgtcagcag tccgatggcc ctcgcgatat taggcgagga cggaagcata agcaggggca
      541 aacgccgaag cgaatgtttg cttagctttt gaaaataagt ttgcacttat gatcttggca
      601 caaggattag cgagtccaat gccatgctcg gcttgcgtat tttgagagta caaattgtcg
      661 aaaccaggca tccccaagtt tgaatttttc ttaaaaaaat tcaaacttgg aaattccaat
      721 ttccaatatc caatttccaa caaaaatcta aatttctaat tcgtaaatcc aaaattttga
      781 catttggatt tggacatttt attagaaatt agaaattggg aatttaagtt attgtctatc
      841 ttttcaaagc cctttcaatt tttctatcac tttctctttt ttgtattaca tcgcgcttgt
      901 caaacttctt tcttcccttt cctatgccaa attccagctt caaaagacct cgtttagtat
      961 atacctgaat gggtacgagt gtcaaccctt cgacccgaga tttcccaatc aaactggcaa
     1021 tttcggatcg gtggagcatc aattttcggg gccgagtggg ctcatagctc ggaatggctc
     1081 ctgcatgctt gtataaagga atattggcgt ttgtgagaaa gagttcttcg ccccgcacgg
     1141 tcacgaaact gcccttgagg ctgatatgcc cagttttcac cgcttttacc tcatatccac
     1201 gcaaaacaat cccggcttca aatctttccg agatgtcata atcgaaactg gctcgtttgt
     1261 tgacggcgag tacgctcata ctcttattaa agcgaaaaat tgatttttag gcaattattt
     1321 tggtatacta ataaagtatg aaaactgaaa ttcaacaact tatccagcaa gccatcaaat
     1381 ccgcgtttgg gatggatttt gatgtggaga aaataaaagt cgattatcca cccgagggga
     1441 tgggggattt ttcgacgaat gtggcattgc ttttggcgaa agaaatcggg aagagtccga
     1501 tggaagtggc ggaggagttg agagataaaa ttgctctcaa ggacgtaggg cttgaaaaag
     1561 tcgaagttgc gctgcccggt tttttgaatt tatacctcgc ggaaaaggct gtgcaggaag
     1621 ttattgcgga aataaacgag aaaggcagtc aatttggaaa cttggaaaag aaaaacgaaa
     1681 aaataatgat cgagtattcc cagccgaaca cgcacaagga atttcacatc ggtcatttga
     1741 gaaatgtatt cattgggtct acgttagtta acgtcctaaa aaaagcgggc agcgatgtca
     1801 tcgctgcgaa ctatatcggt gacactggga cgcatattgc caagtgtctt tggggcttgg
     1861 tcaagttcca tccgggcgaa gacctttcga aaattgaaaa caaaacggaa tttttgggaa
     1921 aagtttattc agaagcggtg caaaaaattg agagcgatga aaagtataaa gacgaattca
     1981 aacaaattca aaaaaggctg gaagacggcg atgaggagat cgtcaaattg tggaaagaaa
     2041 cgcggcagtg gagccttgat gagttcagcg cgatatacaa agaattggga gtttcttttg
     2101 atgaaatatt ctatgaaagc gaagaggaga tagccggcaa gaaaatgctt ccgaatttac
     2161 ttgagaaagg cgttgcgaaa aaaagccagg gagccgtcat tgccgacctt gaagaatatg
     2221 atcttggggt gctggttctt caaagggggg atggcagcgt tttgtatggg ctcaaggata
     2281 ttcctctcgc aattgagaaa tttgaaaaat ataagatcga cggtagcgtg attgtcgcgg
     2341 atgtccggca aagtttgtat tttcggcaga ttttcaaaat tcttgagcaa atgggtttcg
     2401 aaaagaagat ggtacatatc ggatatgaat ttgtagccct caagggcggg gaaagtatgt
     2461 cctcgcgcaa ggggaacgtg gttccggctc aatatttgat tgccgcggtc gcaaatgagg
     2521 tgaaaagaaa atttcctgaa acggatattt cttccgagat tggcctagga gcagtgaaat
     2581 tttttatgct gaaatattcg agcggcacga aaatagattt tgatatcaat gaatcagtga
     2641 aaatcgaggg aacaaccggg ccatacgtcc aatacgccca tgcgagaatt gcgagtattt
     2701 tgcaaaaagc tagtttgcta aggcttagcc ttagcggcgg ctcgctaagg ctaagcctta
     2761 gcaacgttag caataaaaga gaacgagccc tgattttcga acttcttaaa tttcccgatt
     2821 tgattgaaga aatatcgcgt aattttgaag tgcatcatct ctcgaaatat gccactgatc
     2881 ttgcggacaa atttcattct ttctataacg attgcaaagt gatcgatgaa acaaaccctg
     2941 aacttacagc cgcacgttta gaactcgtga aagccgtcca aattgtcctc gcggaagttt
     3001 tgcgattgat gggaatcagc gcgccggata aaatgtaaat attaaaaatg atccatgagc
     3061 gactattata gcaacggata tagcaacgcc cccagaaaaa aaatgtttct cgacattgaa
     3121 actcttcccg cgccggagca tatgcacgaa gtgctgaaag aaatccatgc atacaagaaa
     3181 aagaagaaga ctcgaaacgg cgaaaaatat gcgacgagtt tcgaaagttt tctcgccggg
     3241 acgggactcg acggaacatt cggaagaatt ctttgtattt cgtatgccat caatgacgat
     3301 atgatccatt gcatctgcga tgaagacgag aaaaaagttt tggaaaattt ttgggaagca
     3361 gcgaaagacg ttgatctctt tgtcggtttc aatatcatcg agtttgatat gaaatatctt
     3421 gtccagcgtt cgattgttca cggagtgaaa ccgaaaacaa aaatgcgctt gtcgtttgcg
     3481 aaatatcgta gcgagccgat gtatgacgtg atgcatgaat ggaaccaatg gagcttcggg
     3541 aagggagtgg ggctcgacac attggctcgc gcgcttggct ttccga
//
LOCUS       DATKGS010000119         2722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_195316, whole genome shotgun sequence.
ACCESSION   DATKGS010000119 DATKGS010000000
VERSION     DATKGS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195316"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VMQ48_02955"
     CDS             <1..1635
                     /locus_tag="VMQ48_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HUD09019.1"
                     /translation="GGGAKLAIPKDIDFFYRAKGCSKCHGLGYKGRIGIFEIFTMNEE
                     ISQKITEMAPEGEILALSLEDGMITMLQDGILKALEGITSLEEVQRVTGEGKFLEELY
                     EKIISQLLLRSIAITAEDAEAIQGAKKDLSALGELIAGTSMENALRYILGTGLWLNAG
                     DIHIEPEETTVKIRYRIDGILQDVAEIPMNEYPHFLGNIKVLSGFKATDVEQGVKDSR
                     FGIRLNDGALPEVADREVDVRVSIILGGYGETVVMRLLNQSAEALDLEKLGIRKQNLD
                     LLLAEAKKPNGVILNTGPTGSGKTTTLYSLLSILNKPGVKIITVEDPIEYRLKGVLQT
                     QTDDKKGYTFPKALRALLRQNPDIMMIGEIRDDETAQIAVQSSLTGHLVLSTLHTNNA
                     AGSVQRLINMGVSPTDIASSVNAFMAQRLVRTLCPECKKKITPSPEVKTHIEKILQNI
                     SPKADIEVPKEIGSIFEAVGCPKCNNIGYKGRTTVSEILAMTKEMEELITKQPTTSEV
                     EELARNQGMITMAQDGILKVLEGLTTMEEVERVTEE"
     gene            1745..>2722
                     /locus_tag="VMQ48_02960"
     CDS             1745..>2722
                     /locus_tag="VMQ48_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD09020.1"
                     /translation="MNISRFYQDLDHYLKPGKVLVLYGSRQAGKTTLLRDFLARTKMK
                     YKLDSGDNIKTQEILGSQDFSKILEYASGYDLLAVDEAQRIPNVGQGLKIIVDQIPKI
                     KVIATGSSSFELAGQIGEPLTGRKRTLTLFPLAQMELAGKYNLHEIKERLPEWLIFGG
                     YPEVVTAGTNQEKKDALEEIVSSYLLKDILELERVKGSKILLDLLRLIAFQIGSEVSL
                     TEIGQKMGLDHKTVARYLDLLEKSFVVFNLRGFSKNLRKEITKKSKYYFFDNGIRNAV
                     ISNFNDLDLRDDIGKLWENFLAAERLKTQEYRKISANNYFWRTWDQKELP"
BASE COUNT          826 a          623 c          654 g          619 t
ORIGIN      
        1 ggaggagggg ccaaactcgc gattccgaaa gacatcgact ttttctatcg agcgaaagga
       61 tgttcaaaat gccacggact cggatacaag ggacggatcg gaattttcga aattttcacg
      121 atgaacgagg aaatttccca aaaaatcacc gaaatggcac cggagggaga aattttggcg
      181 ctgtctcttg aagacggaat gatcaccatg ctccaggacg gcattttgaa ggcgcttgaa
      241 ggcatcactt cgcttgagga agtgcaaaga gtgacaggtg aaggcaagtt ccttgaagaa
      301 ctttatgaaa aaataatcag ccagctgctt ttgcgctcaa tcgcgatcac ggccgaagac
      361 gcggaggcga tccagggcgc gaaaaaagat ctttcggctc tcggagaatt gatcgctgga
      421 acttcgatgg aaaacgccct gcgctatatt cttggcactg gcctttggct caatgccggc
      481 gatatccaca tcgaaccgga agagaccact gtcaagatcc gctatcggat cgacggaatt
      541 ttgcaagacg tggcggaaat tcccatgaac gagtatccgc acttcttggg aaacatcaag
      601 gtcttgagcg gcttcaaggc aacggacgtt gaacaggggg tgaaggacag tcggtttgga
      661 atccgtctca atgatggagc tctcccggag gtagccgacc gcgaagtgga tgtccgcgtt
      721 tcgatcattt tgggcggcta tggcgaaaca gtcgtgatgc ggcttctcaa ccagtcggcc
      781 gaagctctgg atctggaaaa attgggaatc cgaaaacaaa atcttgattt gcttcttgca
      841 gaggccaaaa aaccaaacgg cgtgatcctg aataccggcc cgacgggcag cggaaaaaca
      901 acaacgctct attccctgct ctccattctc aataagccgg gcgtgaagat catcactgtc
      961 gaagatccga tcgagtatag attgaaagga gtcttgcaaa cgcaaacgga cgacaaaaag
     1021 ggctatactt ttccgaaagc tttacgggcg ctcctgcgcc agaacccgga cattatgatg
     1081 atcggtgaaa ttcgcgacga tgaaacggcg cagatcgccg tgcaatcttc gctcaccgga
     1141 cacttagtgc tctcaacgct ccacacaaac aatgcggcgg gctcagtaca acgcttaata
     1201 aatatgggtg tctcgccgac ggacattgct tcttcggtca acgcttttat ggcgcaacgc
     1261 ctggttcgca ccctgtgccc cgaatgcaag aaaaaaatca ctcccagtcc cgaagtgaaa
     1321 acccatatcg aaaaaattct tcaaaatatt tccccgaaag cggacattga agttccaaag
     1381 gaaatcggaa gcatttttga agccgtcggc tgccccaaat gcaacaacat tggatacaaa
     1441 ggccgcacaa ccgtgagtga aatccttgcg atgaccaaag aaatggaaga attgataaca
     1501 aaacagccaa ccacttcgga agtcgaagag ctggcccgca atcaaggcat gatcactatg
     1561 gctcaagacg gaattttgaa agtcctcgaa ggcctcacga cgatggaaga agtggaaaga
     1621 gtgacggaag agtaattgcc ttttataaag caaaatataa acaaaatact tgaaataagg
     1681 ttatttcgag tattgctaat ccctataatt tgacaattat ggggatttag actatactat
     1741 agacatgaat atatcccgct tttatcaaga tttggatcat tatttgaagc ccggcaaagt
     1801 cttggttttg tacggttccc gccaggcggg caagactacc ctgcttcgtg attttttggc
     1861 gcgaacaaaa atgaaataca agcttgactc cggcgacaat atcaagacgc aggaaatcct
     1921 cggttcccaa gatttttcaa aaatactgga atacgcttcc ggttatgatc tgctcgccgt
     1981 tgacgaagcg caaagaattc ccaatgtcgg acaaggactc aaaatcatcg tcgatcagat
     2041 cccaaaaata aaagttatcg ccaccggctc atcatctttc gaactggccg gccaaatcgg
     2101 cgaacctctt accggaagga aaagaaccct cacacttttc ccgcttgctc aaatggaact
     2161 tgccggaaaa tataatcttc acgagatcaa agagcgattg ccggaatggc tgattttcgg
     2221 cggatatccc gaggtcgtca ccgccggaac aaatcaagaa aagaaagacg cgctggaaga
     2281 aatcgtcagc tcctatcttt tgaaagacat cttggaactt gaacgggtca agggatcaaa
     2341 aattttgctc gacctcctgc gcttgatcgc ctttcagatt ggaagcgaag tttctttgac
     2401 ggaaatcgga caaaaaatgg gattggatca caaaacagtg gccagatatt tggatcttct
     2461 ggaaaaatca tttgtagttt tcaatttgcg cggtttctcc aaaaatttga gaaaagagat
     2521 cacaaagaaa agcaaatatt atttttttga caacggaatc cggaacgcgg ttatatccaa
     2581 tttcaatgat ttggatcttc gcgatgacat tggaaaattg tgggaaaatt ttctcgcggc
     2641 ggaaaggctc aaaacgcagg agtaccgaaa aatatcggcc aacaattatt tctggcggac
     2701 ttgggatcaa aaagagctcc cc
//
LOCUS       DATKGS010000120        19220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_21595, whole genome shotgun sequence.
ACCESSION   DATKGS010000120 DATKGS010000000
VERSION     DATKGS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 19220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19220
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21595"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VMQ48_02965"
     CDS             <1..391
                     /locus_tag="VMQ48_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUD09021.1"
                     /translation="VLMPKLKIDITKIKEVISNMVDNSIKYTQKGGITVKLETNTTWD
                     TEHKEHKESVRVIVSDTGIGMDAEEIGMIFEKFQRGKQVAHYHTDGTGLGMYIGKKIV
                     AEHKGRIWAESDGKGMGSRFILELPVG"
     gene            674..964
                     /locus_tag="VMQ48_02970"
     CDS             674..964
                     /locus_tag="VMQ48_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HUD09022.1"
                     /translation="MSIKEADKMFSEVKNAKDEFGWKPKITMTTWTLEVEGYSILIYP
                     NGNTYAWPVYDQKDKVLLLGNLLNESISEIWERYPYKESHINKYLGKNIHLS"
     gene            982..1299
                     /locus_tag="VMQ48_02975"
     CDS             982..1299
                     /locus_tag="VMQ48_02975"
                     /inference="COORDINATES: protein motif:HMM:NF020341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HUD09023.1"
                     /translation="MPNLKNQPTLKDFQGYVKELETERGFTDDSVLQKCLMLGEEIGE
                     LFKSIRKSEKIKIDKNSNFKEIDEEIADVIIFLCSIANRYNIDMEKAFREKEEKNKKR
                     IWK"
     gene            1442..1837
                     /locus_tag="VMQ48_02980"
     CDS             1442..1837
                     /locus_tag="VMQ48_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09024.1"
                     /translation="MTKAERKKFIKDTFSATEVGVLIEDMQNGIQVIGEQYGDLKKEI
                     KGIKSELGEFKDEMGEFRNETKANFKAVFDYLSRIEDDFVALKEKVKKLDNEETNSKD
                     FKWLKNKVLEIEKKLEEYKKQQTMLSAKM"
     gene            1919..2224
                     /locus_tag="VMQ48_02985"
     CDS             1919..2224
                     /locus_tag="VMQ48_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HUD09025.1"
                     /translation="MDFKELQGKVVQNAMDYGKKYDVKIDEEFALLKLYEEVGELAQA
                     VLIHKKKSRPEKFVSEEISKNELANELADIMGLLIVNAHLLGIDLEEAIERKWIKRK"
     gene            2206..2526
                     /locus_tag="VMQ48_02990"
     CDS             2206..2526
                     /locus_tag="VMQ48_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09026.1"
                     /translation="MDKAEIKKSMTDKPKKWKALSRETIFQKYGREIEKVMFRLPDGT
                     ESDFYIKKEGPANCVLALTKDNQVILAKQYRPGPDEIVLELQSGKTTDVEVGYLGLDH
                     LGLL"
     gene            2576..3163
                     /locus_tag="VMQ48_02995"
     CDS             2576..3163
                     /locus_tag="VMQ48_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09027.1"
                     /translation="MSRENIEKLAHNWISAKPDEVDVQSEYYCQTRDVFQTRIDRMLR
                     NLLSVEKIGDNRSYIISAIAGEIGNNSFDHNLGNWRDIPGIFFAYEILGSEVKIALVD
                     RGQGLFATLEKVKPELSDDQEALKTAFLERISGRAPENRGNGLKFVKENVEEMKMHLL
                     FYSGSAQAELNAKMIVQETSEKFEGCLAILSVKLI"
     gene            3168..3449
                     /locus_tag="VMQ48_03000"
     CDS             3168..3449
                     /locus_tag="VMQ48_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HUD09028.1"
                     /translation="MKIELKKFGEILISRPAGREAFLAMSAYLTKGLDKNEAIEIDFS
                     GVKVLTPSWADEVVTKIAERFENVKLLNTENATVQATLKTLREYSDLKI"
     gene            3538..4818
                     /locus_tag="VMQ48_03005"
     CDS             3538..4818
                     /locus_tag="VMQ48_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HUD09029.1"
                     /translation="MAEKNEKVCVIGLGYVGFPLAVQCAAKGYEVFGYDKDEKKLSRI
                     EKGEIIVDEEYLDALLPKTRINIIRDEKELGQCDIFVIAVPTPVDEKYYPDLGFVEAA
                     SKTVAENMKSGALVVVESTINPGVCEEVVRPIFEKAGWKVGETVFISHCPERINPGDP
                     KWNVTNIPRVVGSFDEKGLEKSVKFYESILDAPVMPMKTIREAEAAKIIENSFRDVNI
                     AFVNELAKSFDLMGIDIVNVIRGAATKPFAFMPHWPSRGVGGHCIPVDPYYLIEKAKE
                     NDFDHKFLRMARTINNSMPAYTVDILQEMLNQIEKSVKGTNIGVLGVSYKANVGDLRE
                     SPALKVIEILEKHGAKLHIFDPYLPEHSNVKDIDELLEKSEAVVLLTNHKEFLEIKPE
                     KYKEKNIKAILDGMNCLDKEEFLKAGLIFKGIGR"
     gene            4865..5692
                     /locus_tag="VMQ48_03010"
     CDS             4865..5692
                     /locus_tag="VMQ48_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUD09030.1"
                     /translation="MHKIIVVEDDPMLGEIYVKKFTEAGFEMKLVTSGKAVESEVANY
                     QPEIVLLDIVLPDMDGYEILEALSKNNLTRSVPTYVFSNLSAKEDIERATSLGAKGFI
                     TKSSFTPSQLVEEVKKILGSQGAETAAAPAPAETPTSSGAEKVISNQQESNGNKILII
                     EDEDIFIEMFGDKLKQEGFEVVSAKNGKWGLKEAENGGFSCILLDMMMPAMNGYEAVQ
                     ELRANEKTKETPIIILSNSALDSEVQQALALGANEYYIKTQVTPGEVADRVKELIKK"
     gene            5695..7077
                     /gene="aspS"
                     /locus_tag="VMQ48_03015"
     CDS             5695..7077
                     /gene="aspS"
                     /locus_tag="VMQ48_03015"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HUD09031.1"
                     /translation="MERTLISDTVNKIDETVKISGWVKTRRDHGKIIFIDLRDRSGVL
                     QCVITPDKKETYDLAQDIRSEFVLEFEGLVKKRPDNAINTDIETGKVEMEVTNINILN
                     ESKTPPFEIEDTNGVNEEIRLKYRYLDLRTDRMRRNIIERAKIIKFIRDFYTARDFVE
                     VETPILTKGTPEGAREFIVPSRLHPGKFYVLPQSPQQFKQLLMVAGIERYFQIAKCFR
                     DEDQRGDRQPEFTQLDVEMSFSDEKEIRSIFEECLIELIKKYYPEKKIKEIPFPEISY
                     ADAMEKYNSDKPDLRDDKNDANELAFCWIINFPLFERDKEGNLGSSHHPFTAPIDEDL
                     NFLSKEPDKVRAQAYDIVLNGYEIGGGSIRIHKKELQHKIFEVLGLDEKTIQERFSHI
                     LEAFEYGAPPHGGIAPGIDRLAMLLMGEPNIREVMAFPKTGEAEDLMMGAPSEIDEKH
                     LKEVKIKRIF"
     gene            7086..8279
                     /locus_tag="VMQ48_03020"
     CDS             7086..8279
                     /locus_tag="VMQ48_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUD09032.1"
                     /translation="MTSKHKEKLNNPAIRTLDISSFIFGISSALVLYLESDYFKTATK
                     SDNVTLFFIFAYTITLVLIFNWHHLVRKFGKKNVFLADFLVKAIFLLALAKLPVGKIG
                     IWFLMGYMILTVLSWIDIDILLEACSRDHKTGRIRGKYLTIMNAGYLVAPFFAGLMLN
                     KWGFQPVFLTASVLILIVWIICFFKLRNIDGSQVKSVNFIELLRKLSNRKNVLRIYYI
                     SFLLEFFYALMIIYTPLYFLDLGFSWADIGKIFTVMLLPFVLLQYPAGYLADKKFEER
                     DMIIFSLVLMAFSTLVIFFLSSKSVILWALILFCTRIGASLIEILRDSYFYKRIDCRD
                     VDIINFFRSVRPMAYIIGLLIATPIVYFWHIRFVFPLISIGVLTGIFAAKNMASNRIP
                     PQTKR"
     gene            8363..9067
                     /locus_tag="VMQ48_03025"
     CDS             8363..9067
                     /locus_tag="VMQ48_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HUD09033.1"
                     /translation="MFKVQLEKFEGPLDLLLKLIEEQKLDITRLNLAKVADDYLEYIR
                     ANENISLENLAEFVNIASRIILIKSQNILPSLEITAEEEKEIVDLENQLREYQKFKKA
                     GEKIGETLKKKRFSFSRDYLLGVAAAYFPPKNVNVFDLKKAFLKVVSEIVLPEKMPEE
                     SVREIITLEDKIEELKESLQKRVETSFRELSSSAENKIEIIVAFLAMLEMVKQRIIDV
                     EQNELFDEIKIAKVNV"
     gene            9060..9674
                     /gene="scpB"
                     /locus_tag="VMQ48_03030"
     CDS             9060..9674
                     /gene="scpB"
                     /locus_tag="VMQ48_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HUD09034.1"
                     /translation="MSEKNSLKSSIESLLFVSGEPMKLAKIVKICEVSKDDVAREIET
                     LNADYKNSESGFSIIRKNDSVQLATNPANSEAVSRLVSGELQSDLSRSTLEVLAIIAY
                     RGPITRVQIDTIRGVNCSYVLRGLMVKGLVERKETADIRGYLYEISFDFLKSLGIANV
                     QELPDWENLSKNEKVEELLNDSAEEAIADEQTAESIPNDINEQP"
     gene            9671..10699
                     /locus_tag="VMQ48_03035"
     CDS             9671..10699
                     /locus_tag="VMQ48_03035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HUD09035.1"
                     /translation="MSIFVALTTLIFLPAQFYFTGLFLKPPVQTAVPTEKVEWASDSK
                     EQSASQSFKDTIVEQKKNDTVLRESGFLPQKKTDYADTKVLAGASVVIDVDSGTILHF
                     DKGRDHMPIASLTKIMTAVLVMEKVPDLNEVVTIDKEALSADGAKVGCPTSILSKCTS
                     ERLHNGEKITVSDLLTAMLIDSANDAAVALGKHVAGSQKAFAEMMNQKAEELNLGDSH
                     FCNPSGLDVKGCYSSAYDLARIAAYSMRYDNLWKIMNTQESSVQSCDGKYEHSLKNTD
                     MLLGQIPNCLGGKTGFTYNAGKSLMLAATDPETEKHRIIAVILNDNNRWKDMQDLIDW
                     TFNNYEWK"
     gene            10686..11792
                     /gene="mraY"
                     /locus_tag="VMQ48_03040"
     CDS             10686..11792
                     /gene="mraY"
                     /locus_tag="VMQ48_03040"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HUD09036.1"
                     /translation="MSGNNFMHFADFTTIPAAMAALKIWILGTSAFLLAMAWTPLFTK
                     FLYKYKIGVKIKESSVDGKVAPVYHELHKNKSGTPTMGGALVWITVLLLAIIFHLVHP
                     FFGSKIVTRLDFLNRSQTWLPLFVLITTAIIGAFDDIFSVKGWGSNKGGGVRFLYRFG
                     WLFLIAIAGAFWFYYKLGHSVIHVPGFGDIQADLWFIPFFIFVVLATAISSNETDGLD
                     GLNGGILLIAFAVFGAIAFAQNMFNLAAFCSAICGALLAFLWFNIYPARFFMGDTGSI
                     SLGATLAVVALMTNSALILPIVCVIYVIESLSVAIQLTSKKLFKKKVFLSTPIHHHFE
                     AIGWPETKITMRAWIFTGVAAGMGLVIGILGMGK"
     gene            11841..12884
                     /locus_tag="VMQ48_03045"
     CDS             11841..12884
                     /locus_tag="VMQ48_03045"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HUD09037.1"
                     /translation="MKYLKDVDFKNKRVLLRTDFNVPLENGKILDDFRIHESLPTIEH
                     ILKQDKSKIVILSHLGRPEGKIDSQYSLEPVAKKLEELLKREIIFIKDILSPEGDEII
                     QSLEGGQIAFAENLRFWPQEEEGDEKFAIDLCHHFGVFVNDAFSDSHRAHASISQIPR
                     FKPSCAGLLMQKEIEELSKALKPSKRPAIVVIGGAKIETKMPVIENMAGNYDAVLVGG
                     RIAVEVQGQSLKFSENVILPEDYVDETLDIGPKTAEKYRMAISSASFVVWNGPMGKFE
                     DEKYAAGSKAILDAICESDAWKIAGGGETVEFIDKQKQADKFDFISSGGGAMLEFLAG
                     DKLPGIEALENSI"
     gene            12903..14150
                     /gene="eno"
                     /locus_tag="VMQ48_03050"
     CDS             12903..14150
                     /gene="eno"
                     /locus_tag="VMQ48_03050"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUD09038.1"
                     /translation="MPKIKKISAIEILDSRGNPTVKAVVTLENNISGSASIPSGASTG
                     THEALELRDDDTRYGGLGVLNACENINKTINKKLAGFDVSDQKKIDAAMIKLDGMENK
                     SNLGANAMLAVSLATARTAATAQNLPLYKYLAKKYGFKGPKKIPTAFFNIINGGAHAD
                     SGLSVQEFQIVPEKFKSFAEKLRAASEIFHKLKSFFANAGYPIGVGDEGGFAPQLDSH
                     SDAFEYIQKAIEEAGYKNKVSLGIDAAASSFFDENEKRYTLKPENTSLEADRLVSLYQ
                     EWVKKYKIISLEDGLSEDDFENWKKLNDKLGKQALIIGDDLLVTNMDRLKKALSFSAI
                     NAAIVKPNQIGTLTETMDFIKLCQKNKIKTVVSHRSGETCDDFIADLAVAAGAEFVKF
                     GAPCRGERTAKYNRLLEIEKEIK"
     gene            14147..15676
                     /gene="gpmI"
                     /locus_tag="VMQ48_03055"
     CDS             14147..15676
                     /gene="gpmI"
                     /locus_tag="VMQ48_03055"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HUD09039.1"
                     /translation="MMKKNKPTILAILDGWGIGPKDDTNAIWLARTPVFDYLTKRYPF
                     TQLGATGKDVGLDDRQTSGSETGHMNIGAGRIIQQDSRKISESINTGTFFHNAAFLGA
                     ISHVKKNKSNLHLAGLLGNEDSPHMNPYHLEALLLLAKQNGIKNVFIHFFTDGRDSYP
                     KSAFSHLDDWEKRIKHIGVGKIATLVGRFYAMDRAKNWDRLKVAYDLLVGGKGKKFKY
                     PKDAVVDSYNNALTDEYIKPSVIIPKAKIKNNDAVIFFNLRSDRARQFAKLFVLEKSK
                     EIKLPKPRLKNLYFVAMTNFGPDLPVHTAFQDHPVNGTLPTALGDFRQLYIAETEKFA
                     HITYFLNGGYADAIDGEERLMIPSPITDNYAKLPQMSAEKITQKILGFLRKNKYDFTA
                     VNFANADMVGHTGNMKATIKAAEVVDKCLGKIFKELQRHNGNLIVTADHGNADCMWDK
                     RVNLPMTFHTKNPVPLIIASDVCKKKKLASGGVLGNISPTICDIMRTEKLKEMSKKSL
                     L"
     gene            15687..16565
                     /locus_tag="VMQ48_03060"
     CDS             15687..16565
                     /locus_tag="VMQ48_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HUD09040.1"
                     /translation="MYDLISIGSITRDIFVPTDRGKIFKTPKDKLAPVWIGFELGEKI
                     CIDEVIETGGGVATNLSIGTKKLGLKSAPLGPAKPATSVILIDQKTGERVIFYQKSSG
                     TINIDKLSKIKTGWLSISSLTGKWTKQAGQISNYAAKNKTGLILLPSTSQIRDDFPDL
                     KKLLKTAKILILNKNEALEIASKMKIKANNTENLFKLLHKLGPEIICITDGTKGAWAS
                     DEKNIFHSPIQKVKAVDVTGAGDAFASGFLGFYLKGGNLKTSLRAGIFNSASVVKYIG
                     TTKGLLAKKDILAKIK"
     gene            16562..18649
                     /locus_tag="VMQ48_03065"
     CDS             16562..18649
                     /locus_tag="VMQ48_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HUD09041.1"
                     /translation="MNKLDHFFSPKSIAIVGASPKRGKLGNVLIENIKKGGWKGKLYF
                     VNPKYAKSRIGYFSNLSEIKNPVDLVLVAIPAPFAGEILENGAKAKPAIKNFAVISSG
                     FKEAGKEGAILEKNLANIAQKYNLNVLGPNCLGFSNPAEKLNATFTDVKLQGGSIAIV
                     SQSGALAVALLDWAESAKIGFSKIVSIGNKADLEESEILNFLSSDKNTRAIALYLEDI
                     KNGPRFMEALAKITPRKPVVVLKAGKTAVGQKAVSSHTGSLAQDEEIIEAVFEKTNVV
                     PANNIEEFQDIIYYLNSNPVPAKNEMIILTNAGGPGVMASDFIGKSKSIKLLDFPEKF
                     KNEIKKYLPTSASVNNPIDVIGDAPPERYAKTLEHIAKYFPKNPVLVILTPQNQTDPK
                     KVAQILVKFRSKIENMSICFMGGEKIARAKEFLAKKKIPNFENPERALAVIEKLAKYS
                     QSQSKDAFNASSLHNRRTQCLSIKPNLIIQKALTEKRRIIFWKEAGKLFGKFQVKLAR
                     SISFESIIELKNKKIPFPCALKTDDPNIAHRLEKNAVMLDIQNAKDLNDTFEKMKKAT
                     GVKHFLVQTMADPGLEMIIGMKNDPTFGPVILCGWGGSFTEIFKDKVILVPPFDKKEI
                     ANKLEQLAIFPILKGFRGKKGYDLAEIAKVIAATAQIALENPQIREIDINPLVVYNDG
                     KEGKILDAKIFLT"
     gene            18654..>19220
                     /locus_tag="VMQ48_03070"
     CDS             18654..>19220
                     /locus_tag="VMQ48_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUD09042.1"
                     /translation="MNEQNKFKILIVDDDDIIRETYRQVFQEHGFESISAKDGVEGID
                     LATKEKFDVILTGIIMPRMDGFQLISALRENVDTKDIPMAIISHLGREEDRRRSKEMG
                     IENFIVQGTMTPREVVDKMKSLIASGSSFQLGFDYSAWDAPALAKKMDLPGFECPSCQ
                     NKMILELTPKSEGKGFDAFFKCPKCDLHP"
BASE COUNT         6029 a         3820 c         4426 g         4945 t
ORIGIN      
        1 cgtactgatg cctaaattga aaattgacat cacgaagatc aaggaagtga tctcgaatat
       61 ggtggacaac tcgatcaaat atacccaaaa aggcggaatc acggtgaaat tggaaaccaa
      121 tacgacttgg gacacggagc acaaggaaca caaggaatct gttcgcgtta tcgtttccga
      181 cacgggaatc ggcatggacg cggaagaaat cggaatgatc tttgagaaat tccagcgcgg
      241 gaagcaagtg gcgcactatc acaccgacgg gacggggctg ggaatgtata tcggcaagaa
      301 aatcgtcgcc gagcacaaag gccgcatctg ggcggaaagt gatgggaaag gaatgggaag
      361 ccgatttata ttggagttgc cggtggggta atttagtttt ttctcgaaat gagtaaagta
      421 aacttcaata caaacttttg cagatttgaa tatcacgaat aggataaggg gaaattacga
      481 caaaatattc aagggcatag gtataataaa aaagcacaaa attccccttg ctctttgcag
      541 tgtggtttta tcaagtacga aaaattcaat tttgtatgcc tgccaaattg ctgatgtact
      601 ggaagcaaaa aaatttaaat tgattcttcc tgttccgaag ggcaatgctt tgaatttgcc
      661 aaagcgggaa tatatgtcaa tcaaagaagc agataaaatg ttcagtgaag taaagaacgc
      721 aaaagatgag tttggctgga aaccaaaaat caccatgact acgtggactc tggaagttga
      781 gggatattcc attctgattt atccaaacgg aaatacttac gcatggccgg tttatgatca
      841 aaaggacaaa gttctgcttc taggaaatct tctgaatgag tcgataagtg aaatatggga
      901 acgatatccc tataaagaaa gccacatcaa caaatatttg gggaaaaata ttcatttatc
      961 ttaaaaataa tattcaatat aatgccaaat ttaaaaaacc agccgacgct aaaagatttt
     1021 caagggtatg ttaaagaact ggaaacagaa agaggattta ctgacgatag cgtgcttcaa
     1081 aaatgtttga tgctaggcga agaaatcgga gagttgttca aatcaatcag aaaaagtgaa
     1141 aagataaaga tagacaaaaa ttcaaatttc aaagaaattg atgaggaaat cgccgacgtt
     1201 ataatttttt tgtgctcaat tgcaaatcgc tacaatatag acatggaaaa agccttcagg
     1261 gaaaaggaag aaaaaaacaa aaaaagaatt tggaaataat ttgttaatac gagttgatat
     1321 cagccgcaaa atcgtcgccg agcacaaagg caaaatctgg gcggaaagcg acgggaaagg
     1381 catgggttcg aggttcatct tggagttgcc ggtggggtga tgatttgcta taatatttcg
     1441 tatgacaaaa gctgaaagaa aaaaattcat caaagacacc ttttccgcta cagaggtcgg
     1501 tgttcttatt gaagatatgc agaacggaat acaggttatc ggagaacaat atggagactt
     1561 gaaaaaagaa atcaagggaa tcaaaagtga attgggtgaa tttaaggatg agatgggaga
     1621 atttagaaat gaaaccaaag ctaatttcaa ggcagttttt gactatctgt cccgcattga
     1681 ggatgacttt gtcgctctca aagaaaaagt taagaaacta gataatgaag aaacaaattc
     1741 taaagatttc aaatggctga aaaataaggt tttggaaatt gagaaaaaac tcgaggaata
     1801 caaaaagcag caaactatgc tctcggcgaa gatgtaggtt gttttgtctt gcaaatttat
     1861 atctgctgga ctattgttaa ataagcttta accagcttat tttttaatac tatattttat
     1921 ggatttcaag gaactgcagg gcaaagtcgt gcaaaatgcg atggattatg gcaaaaaata
     1981 tgacgtcaaa attgacgagg aatttgcttt gctcaaatta tacgaagaag ttggagaatt
     2041 ggctcaagcg gttttgatcc acaagaagaa aagccgcccc gaaaaattcg tctcggaaga
     2101 aatttcaaaa aatgaactgg caaacgaatt ggcggatata atgggcttgc tgatcgtaaa
     2161 cgcgcatttg ttgggaattg atctggaaga agcaattgaa agaaaatgga taaagcggaa
     2221 ataaagaaat ctatgacaga caaacccaaa aaatggaaag cgctttcccg cgaaactatt
     2281 tttcaaaaat atgggcggga aattgaaaaa gtgatgttca gactgcctga cgggacagag
     2341 agcgactttt atatcaagaa agaagggccc gccaattgcg ttttggctct cacgaaagac
     2401 aatcaagtta ttctggccaa gcaatatcga ccggggccgg atgaaattgt cctggagctc
     2461 caaagcggaa aaacgaccga tgtggaagtg ggatatttgg gattggatca tttgggattg
     2521 ttgtaaatag atttcgtttt tgccttttcg tgccgtttat ggtacgattt ttcatatgag
     2581 tagagaaaat atcgaaaaat tagcgcataa ttggatttct gcaaagcccg acgaggttga
     2641 tgttcagagt gaatattatt gccagacgag ggatgttttt cagacgcgga tcgacagaat
     2701 gttgcggaac ttattgagcg tggagaaaat tggcgataac agatcctata taatttcagc
     2761 cattgccggt gaaatcggga acaattcatt tgatcacaat cttggaaact ggcgggatat
     2821 tccgggaata ttttttgcct atgaaatact tggcagtgag gtcaaaatag ctttagttga
     2881 tagaggacag ggattattcg ctactcttga gaaggtaaag ccggaattat ccgacgatca
     2941 agaagcgctg aaaacggctt ttttggaaag aatttccgga agagctccgg aaaacagagg
     3001 aaacgggttg aaattcgtga aagaaaatgt tgaagaaatg aaaatgcact tgctttttta
     3061 ttcaggaagt gcgcaagctg aattgaacgc aaaaatgatt gttcaagaaa cgagtgaaaa
     3121 attcgaaggc tgtctcgcaa tcctatcggt aaaattaatc taaattcatg aaaattgaac
     3181 tcaaaaaatt tggcgaaatt ctgatttccc gaccggccgg aagagaagca tttttggcca
     3241 tgtctgcata tctgacaaaa ggtttggaca aaaatgaagc cattgaaatt gatttttcag
     3301 gagtgaaggt tttgactccc tcctgggccg atgaggttgt cacaaaaatc gcggagagat
     3361 ttgagaatgt aaaactgctg aatacggaaa acgcgactgt ccaggcgaca ctcaaaactt
     3421 tgcgagaata ttcggatttg aagatatgaa aagctgaaag attacagcgc gggattgccc
     3481 tttcgtatcg ttcgtggtac gattttttag taattattca ctatatataa taaaaatatg
     3541 gcagaaaaaa acgaaaaagt ctgtgtgatc ggtcttgggt atgtcggttt tccgctggcg
     3601 gtgcaatgcg cggcgaaagg atatgaggtt tttggatatg acaaagacga gaaaaaactc
     3661 tcccgtattg aaaaaggtga aattatcgtt gatgaagaat acttggacgc gcttctgccg
     3721 aagacaagaa taaacatcat ccgcgatgaa aaagagctcg gccagtgcga tattttcgta
     3781 atcgccgttc cgacgccggt cgacgaaaaa tattatcccg atttgggctt tgtcgaagcg
     3841 gcttcaaaaa cggtggcgga gaatatgaaa agcggtgcac tcgttgtggt tgagtcgacg
     3901 atcaatccgg gagtttgtga agaagtcgtc cggccgattt ttgaaaaagc gggctggaaa
     3961 gtcggagaaa ctgttttcat ttcccattgt cccgagagga tcaatccggg cgatccgaaa
     4021 tggaatgtga caaatattcc ccgcgtcgtc ggttcatttg atgaaaaggg tctcgaaaag
     4081 tctgtgaaat tctatgaaag tatcttggat gcaccagtga tgcccatgaa aacaatccgg
     4141 gaagctgaag ccgcgaaaat cattgaaaat tctttccggg acgtcaatat cgcttttgtg
     4201 aacgagcttg ccaaatcttt tgatctcatg gggattgaca ttgtgaatgt catccgtggc
     4261 gccgcgacaa aaccttttgc tttcatgccg cattggccga gccgaggagt gggcgggcat
     4321 tgcattccgg tggatccgta ttatctcatt gaaaaagcca aggaaaatga tttcgaccac
     4381 aaatttttgc gaatggcccg gacaatcaac aatagtatgc cggcctatac tgtcgatatt
     4441 ttacaagaaa tgctcaatca gatcgaaaaa tcggtcaaag ggacgaatat cggcgttttg
     4501 ggcgtttcgt acaaagccaa tgtcggtgat ctgcgggaaa gcccggcgct caaggtcatt
     4561 gaaattctgg aaaaacacgg ggcaaaactt catatttttg atccatactt gccggagcat
     4621 tccaatgtga aagatataga cgaattgctc gaaaagtctg aggcggttgt tttgctcacg
     4681 aatcacaaag aatttttgga aataaagccg gaaaaataca aagaaaaaaa tataaaagcc
     4741 attttggatg gtatgaattg cctggacaag gaagaatttc tcaaggccgg tctgattttc
     4801 aagggaattg gaagataagt gaaaaatctg gtataatacg gacataagcc ataagctcaa
     4861 atttatgcat aaaataattg tcgtcgagga tgatccgatg ctcggagaaa tatatgtgaa
     4921 aaaattcacc gaggctggtt ttgaaatgaa actggtgacg agcggcaaag ccgtcgaaag
     4981 cgaagtggca aactatcagc cggaaatagt gcttttagac atcgtgcttc cggacatgga
     5041 cggatatgaa attttggagg ctttgtccaa aaataatctt acccgttccg tcccaaccta
     5101 tgttttcagc aacttgagcg ccaaggaaga tattgagcgg gcaacttcgc tcggcgcaaa
     5161 aggcttcatc accaaatcaa gcttcacacc gtcccaattg gttgaagaag tgaaaaaaat
     5221 cttgggaagc caaggagcag aaactgcagc cgcgcctgcg ccagcggaaa ctccgacttc
     5281 gagcggtgcg gaaaaagtca tttccaacca gcaagaatcg aacggaaaca aaattttgat
     5341 cattgaagac gaagatattt tcattgaaat gttcggcgac aagttgaaac aggaaggctt
     5401 tgaagttgtt tcggccaaaa acggaaaatg gggactcaag gaagcggaaa acgggggatt
     5461 cagttgcatt ttgctcgata tgatgatgcc ggccatgaac ggatatgaag ccgtccagga
     5521 actgcgcgcc aatgaaaaaa caaaagaaac accgatcatt atcctttcca attcggcact
     5581 cgacagcgaa gtccaacaag ctctcgcgct tggagcgaat gaatattata tcaaaaccca
     5641 ggtgacaccg ggagaggtgg cggacagagt gaaagaattg atcaaaaaat agttatggag
     5701 cgaacgttaa tctcagatac agtaaataaa atcgacgaaa ccgtgaaaat atccggctgg
     5761 gtgaagaccc gccgcgacca cggaaagatt atttttatcg acctgcgtga ccgatcgggt
     5821 gttttgcaat gcgtgatcac gccagacaaa aaagaaacgt acgatttggc gcaagacata
     5881 cgaagcgaat ttgtactgga atttgaaggc ttagttaaaa aacgtccgga taatgcaatc
     5941 aatacggata ttgaaacagg aaaagtcgaa atggaagtga caaatatcaa tatcttaaat
     6001 gaatctaaaa cgccgccttt tgagattgaa gacacgaacg gagtaaatga ggaaatcagg
     6061 ttgaaatatc gatatctcga tcttcgaacc gatcgaatgc ggagaaacat catcgagaga
     6121 gcgaaaataa taaaatttat tagagatttt tatactgcaa gggactttgt ggaagttgaa
     6181 actccaatcc tcaccaaagg aacaccggaa ggcgcccgag aatttattgt tccctcacgc
     6241 ctgcatcctg gaaaattcta cgtcctgccc caatcgcctc agcagttcaa acaacttttg
     6301 atggttgcgg gaatcgaaag atacttccaa attgccaaat gtttccgtga tgaagatcaa
     6361 agaggggatc gccaaccaga attcacccag cttgatgttg aaatgagttt ttccgatgaa
     6421 aaagaaattc ggagcatttt tgaagaatgt ttgattgaac ttatcaaaaa atattatcca
     6481 gaaaagaaaa taaaggaaat tccattccca gaaatttcct atgcggatgc aatggaaaaa
     6541 tacaattctg ataaacctga tttaagagat gacaaaaatg acgcgaatga actggctttc
     6601 tgctggatca taaatttccc gctttttgaa agggataagg aaggaaactt gggttcatct
     6661 catcatccat tcactgcgcc aatcgatgag gatcttaatt tcctcagcaa agaacctgat
     6721 aaagtccggg ctcaagcgta tgacattgta ctgaacggct acgagattgg tggtggaagt
     6781 attcgtattc ataagaaaga gctgcagcac aaaatttttg aagttttagg cttggatgaa
     6841 aaaaccatcc aggaaagatt tagccatata cttgaagctt ttgagtatgg cgctcctcct
     6901 catggcggaa tcgctccggg catcgataga ctcgcgatgc ttctcatggg cgagccaaat
     6961 attcgcgaag taatggcttt cccaaaaacc ggagaagcag aggatttgat gatgggagcg
     7021 ccaagtgaaa tagatgaaaa acatctgaaa gaagttaaga taaaaagaat cttctagatt
     7081 ctgtgatgac atcaaaacac aaagaaaaat tgaacaatcc ggctattcga acgctggata
     7141 tctcttcttt tattttcggc atatcgtccg cgctggttct ttatctcgag tcggactatt
     7201 tcaagactgc cacaaaaagc gacaatgtga cgcttttttt tatttttgcc tatacgatca
     7261 cgctcgtttt gattttcaat tggcatcatc tcgtgagaaa attcgggaaa aagaatgttt
     7321 ttttggctga ctttctggta aaagcgatat ttcttctggc gcttgccaaa ttgcccgtcg
     7381 gaaagattgg catctggttt ttgatgggct atatgatcct caccgttctt tcctggattg
     7441 acatcgacat tctgcttgaa gcttgttctc gcgaccacaa aaccggacgc atccgcggca
     7501 aatatctcac catcatgaat gcgggatatc tcgtcgctcc atttttcgcg gggttaatgc
     7561 tcaataaatg gggatttcag cctgtttttt tgaccgcttc agttcttatt ctcatcgtct
     7621 ggataatttg ttttttcaaa ctacgcaata tcgacggtag ccaagtcaag agcgtaaatt
     7681 ttattgaact cttgcgaaaa ttgtccaacc ggaaaaatgt tttgcggata tactatattt
     7741 cgtttcttct tgaatttttc tacgctttga tgatcatcta tacgccgctc tattttctcg
     7801 acctgggatt ctcttgggcg gatattggaa aaatattcac agtgatgctt ttgccgttcg
     7861 tccttttgca gtatcccgcc ggatatctgg ccgacaaaaa attcgaagag cgggatatga
     7921 tcattttttc ccttgttttg atggcttttt caactctggt aatatttttc ctctcctcga
     7981 aaagtgtgat tttgtgggcc ttgatcctat tttgcacccg tatcggagcc agtctcattg
     8041 aaattttgcg ggattcctat ttctacaagc gcatcgattg ccgagacgtg gacatcatca
     8101 attttttccg cagcgttcgg ccgatggcct atataatcgg acttctgatt gccactccga
     8161 tcgtatattt ttggcatatc cgcttcgttt ttcccttgat ttccatcggc gtgctgacgg
     8221 gcatattcgc cgccaaaaac atggcttcaa atcggattcc gccgcaaaca aaacgctgaa
     8281 aatgactgat agtcaaagca tatatttcgg gttgtttttt cgtgccattt gtggtacgat
     8341 ttttgtataa aataaagcgc caatgttcaa ggttcagctc gaaaaattcg aaggtcctct
     8401 tgatttgctt ctcaagctga tcgaagagca aaagttggat attacgcgcc tcaatctggc
     8461 caaagtggcc gatgactatc tcgaatatat ccgtgccaac gaaaacataa gcttggaaaa
     8521 tctggccgaa tttgtcaata tcgcctcaag gatcattctt atcaagtccc agaatatcct
     8581 cccgtctctt gaaatcacgg ccgaagaaga gaaagaaatt gtcgaccttg aaaaccagct
     8641 ccgggaatat cagaaattca aaaaagcggg cgagaaaatt ggggaaacat tgaagaaaaa
     8701 gaggttttct ttctcgagag actatctttt gggggtcgcg gctgcgtatt tccctccgaa
     8761 aaatgtgaat gtgtttgatc tcaagaaagc gtttctcaag gtcgtttcgg aaattgtttt
     8821 gccggaaaaa atgcccgaag agtcagtgag agagattatc actcttgagg acaaaatcga
     8881 agagctgaaa gaatctctcc aaaagagagt ggagaccagt ttccgggaat tgagcagctc
     8941 tgccgagaac aaaattgaaa tcattgtcgc gtttcttgcg atgcttgaaa tggtgaaaca
     9001 gcggatcatc gatgttgagc aaaatgaatt atttgatgag ataaagatag ccaaagtgaa
     9061 tgtctgaaaa aaatagccta aaatcgtcca ttgaaagcct tctctttgtt tccggagagc
     9121 cgatgaagct tgcgaaaatc gtcaagattt gtgaggtttc aaaagatgat gttgcccggg
     9181 aaattgaaac gctcaacgct gattacaaaa atagtgaaag cggtttttct attattcgaa
     9241 aaaatgattc agtgcaactt gccaccaatc ccgcgaattc ggaagccgtt agccggctgg
     9301 tgagcgggga gttgcaatca gatctctccc ggtcgacact tgaggttttg gcgatcattg
     9361 cctatcgcgg accgatcacc cgtgtccaaa tcgatactat ccggggcgtg aattgtagct
     9421 atgttttgcg aggcttgatg gtcaaaggac tggttgagcg caaagaaacg gccgatatcc
     9481 ggggctatct atacgaaatt tcatttgatt ttttgaaaag tttgggaatt gccaatgttc
     9541 aagaattgcc ggattgggaa aacttgtcaa agaatgaaaa agtggaagaa ttgctgaatg
     9601 acagtgcgga agaagcgata gctgacgaac aaacagctga atcgattccc aacgatataa
     9661 atgaacagcc atgagcatct ttgtcgcgct cacaaccctg atttttcttc ccgcccagtt
     9721 ctatttcacc ggactattct tgaagccacc agtccaaaca gctgtgccga cggagaaagt
     9781 tgaatgggct tcggacagca aggaacagtc tgccagccaa tcattcaaag acacaatcgt
     9841 cgaacaaaaa aagaacgata cagttctccg agaatcggga tttcttcctc agaaaaaaac
     9901 ggattatgcc gataccaagg ttttggccgg agcatccgtc gtcatcgatg ttgattcagg
     9961 aacgattctc catttcgaca aggggcgcga ccatatgccg attgcctcac tcaccaagat
    10021 tatgactgcc gttttggtca tggaaaaagt gccggatctc aatgaagttg tgacgatcga
    10081 caaggaagcg ctttccgctg acggagcaaa agtcggctgt cccacgagca ttctttccaa
    10141 gtgtacctcc gaaaggttgc ataatggcga aaagatcacc gtgagcgacc tcttgacggc
    10201 gatgctcatc gacagtgcca atgatgcggc tgttgccctt ggaaaacacg tggcgggatc
    10261 ccagaaagct ttcgcggaaa tgatgaacca aaaagccgaa gaactcaacc tcggagattc
    10321 ccatttttgc aatccgtctg gattggacgt gaaaggatgc tattcttccg cctatgatct
    10381 ggctcgcatc gctgcctatt ctatgagata tgataatctt tggaaaatta tgaatacgca
    10441 ggaatccagc gtgcaatcat gtgatggaaa atatgaacat tccctaaaaa atacagatat
    10501 gctgctgggt caaattccaa attgtcttgg cgggaaaacc ggttttacct acaacgccgg
    10561 gaaatcattg atgctggctg ccaccgatcc cgaaaccgaa aaacacagaa tcatcgctgt
    10621 gattttgaat gacaacaacc gctggaaaga catgcaagac ctgattgatt ggacatttaa
    10681 taattatgag tggaaataat tttatgcatt ttgccgactt cacaaccatt cctgccgcga
    10741 tggccgctct caagatttgg attttgggaa cgtcggcttt tcttttggct atggcctgga
    10801 cgccgctttt cacgaaattt ttatacaaat ataaaattgg cgtcaaaata aaagaatctt
    10861 cggttgatgg aaaagttgct ccggtatacc acgagctcca caaaaacaaa agcgggacgc
    10921 caactatggg tggcgctttg gtttggatca cagtactcct tctggcaatt atttttcatt
    10981 tggtccatcc cttttttgga tcgaagatag ttactcgtct tgatttctta aaccgttctc
    11041 aaacttggct gcccctcttt gtcctgatca cgacagcgat tattggcgct ttcgatgata
    11101 tttttagcgt aaaaggttgg ggatccaaca aaggtggagg agtcaggttt ttgtaccgtt
    11161 tcggatggtt atttttgatt gcaattgctg gcgcgttctg gttttattat aagctgggac
    11221 acagcgtcat ccacgttccc ggttttgggg atatccaggc cgatttgtgg ttcattccct
    11281 tcttcatctt tgtcgttctt gcaaccgcca tttcttcaaa cgaaacagac ggactggacg
    11341 gactcaatgg agggatattg cttatcgctt ttgcggtttt cggcgcgata gcttttgctc
    11401 aaaacatgtt caatctcgct gctttttgca gtgccatctg cggagctctt ctggcctttt
    11461 tgtggttcaa tatctatccg gccagatttt ttatggggga tacaggctcg atttctttgg
    11521 gtgccacttt ggccgttgtc gctcttatga caaattcggc gctgatcctt ccgattgttt
    11581 gcgtcatata tgtgatagaa tccctgtcgg tggcgattca actcacctcg aaaaaattgt
    11641 ttaagaaaaa agttttcctt tcgacaccga tccatcatca ttttgaggca attggttggc
    11701 cggaaacaaa aatcacaatg cgcgcctgga tttttaccgg tgtcgccgcc gggatgggtc
    11761 ttgtgatcgg gatattgggt atggggaaat aatcaaaagt gaatattagt aattgactaa
    11821 aaaattttaa aaatgaaaaa atgaaatatc tcaaagacgt cgatttcaaa aacaaaagag
    11881 tactcttgcg gacggacttt aatgttcctc ttgaaaatgg aaaaattttg gatgattttc
    11941 gcatccatga atcgcttccg acaatagagc atattctgaa acaggacaaa tcaaaaatag
    12001 tcatcctttc tcatcttggc cgtccggagg gaaaaattga ttcacaatat tctcttgaac
    12061 cggttgccaa aaaattggaa gaactgctca agcgcgagat cattttcatc aaagatattc
    12121 ttagtcctga gggagatgaa ataatccaat ctcttgaagg cggacaaatt gctttcgcgg
    12181 aaaatttgcg attttggccg caggaagaag aaggggatga gaaatttgca atcgatcttt
    12241 gtcatcattt cggtgttttc gttaatgatg ctttcagcga ctcgcatcga gcgcacgctt
    12301 cgatctcaca aatcccacgc ttcaagccat cctgcgcggg attgttgatg caaaaagaga
    12361 ttgaagagtt gtcaaaagcg ctcaaaccct caaaaagacc ggcaatcgtc gtcattggcg
    12421 gagccaaaat cgaaaccaag atgcctgtca ttgaaaatat ggccggaaat tatgacgccg
    12481 tgcttgttgg cggaagaatc gccgtcgaag tgcaggggca gtcgctgaaa ttcagtgaaa
    12541 atgtcatttt acccgaagat tatgtggatg aaacgcttga tatcggtccc aaaactgctg
    12601 aaaaatatag aatggccatc tccagcgctt cctttgttgt ctggaacggc ccaatgggaa
    12661 aatttgagga cgaaaaatac gctgctggtt caaaagctat tcttgatgcg atttgtgaat
    12721 ctgacgcttg gaaaattgcc ggcggcggtg aaacagtcga gtttattgat aagcagaagc
    12781 aggctgacaa gtttgatttc atttcatccg gtggaggagc gatgctcgaa tttctggccg
    12841 gcgacaagct tccgggaatt gaagccttgg aaaatagtat ttagtattta gtataaaaaa
    12901 ttatgcccaa aataaaaaaa atttccgcta tcgaaattct cgattcgcgt ggaaatccga
    12961 cggtgaaggc agtagtgacg ctcgaaaata atatttccgg ttcagcctct attccatccg
    13021 gcgcctcaac cggaacacat gaggcactcg aactccgcga tgatgatacg cgctatggtg
    13081 gattgggagt tctcaacgcg tgcgaaaata tcaacaagac tatcaacaaa aagctggccg
    13141 gatttgatgt gtctgatcag aaaaagatcg acgcggcgat gattaaattg gatggaatgg
    13201 aaaacaaatc aaatttggga gccaatgcga tgctggccgt ttcattggcc acggctcgga
    13261 cggccgccac ggcgcaaaat ctgcccttat acaaatattt ggccaaaaaa tacggcttca
    13321 aaggtcccaa aaaaattccg acggcctttt tcaacatcat caacggaggc gctcacgccg
    13381 actccggtct ttccgtgcag gaatttcaaa ttgttcccga aaagttcaaa agttttgctg
    13441 aaaaattacg cgcagcgagt gaaatcttcc acaaattgaa gtcttttttc gctaatgccg
    13501 ggtatccgat tggggtcggc gacgaaggcg gctttgctcc gcagctcgat agccatagtg
    13561 acgcttttga atatattcag aaagcaattg aagaagcggg atacaaaaac aaagtatcat
    13621 tgggaattga tgcggctgct tcaagttttt ttgacgagaa tgaaaaaaga tacacattga
    13681 aaccggaaaa tacaagtttg gaggcagacc gattggtctc tctctatcaa gaatgggtga
    13741 aaaaatacaa aatcatttct cttgaagacg gtctgtcgga ggatgatttt gagaattgga
    13801 agaaattaaa tgataaatta ggaaagcaag cattgattat aggcgatgac ttgcttgtga
    13861 cgaatatgga tcgcctcaaa aaagccttgt cattctcggc gatcaatgca gcaattgtga
    13921 agccgaacca gatcggcacc ctgactgaaa cgatggattt catcaagctt tgtcagaaga
    13981 acaagatcaa gacggtcgtt tctcaccgga gcggtgaaac ttgcgatgat ttcatcgccg
    14041 acctggctgt cgcggctgga gcggaatttg tcaaattcgg cgctccttgc cgcggagaaa
    14101 ggacagcgaa atacaatcgc ttactggaga ttgaaaaaga aatcaaatga tgaaaaaaaa
    14161 taaaccaaca attctggcta ttttggatgg gtgggggatt ggtccaaaag atgatacaaa
    14221 cgcgatttgg ctggccagga cgccggtttt tgattattta acgaaaagat atccgtttac
    14281 acaattgggg gcaacgggaa aagacgtcgg gctcgatgat cgccaaacca gcgggtcgga
    14341 aacaggccat atgaatatcg gcgcaggacg gatcattcag caggattcgc gaaaaatttc
    14401 cgagagcatc aacacgggga ctttttttca caacgccgcg tttctggggg cgatttcgca
    14461 tgtcaaaaaa aacaaatcaa atctgcatct tgcgggactc ctgggaaatg aggacagccc
    14521 gcacatgaat ccctatcatc tcgaggcgct acttctgctc gccaagcaaa acggcatcaa
    14581 aaatgtcttc atccattttt ttacggacgg acgtgattca taccccaaaa gcgctttttc
    14641 ccatttagac gactgggaga agaggattaa gcatatcggc gtggggaaaa tcgcaaccct
    14701 ggtcggaaga ttttatgcca tggatcgcgc caagaattgg gatcgcttga aggtggccta
    14761 tgatctgctt gtgggcggga aaggaaaaaa attcaaatac cccaaagatg ctgtcgttga
    14821 ttcctataac aatgccttga cggacgaata tatcaaaccg tcggtcataa tccccaaagc
    14881 aaagatcaaa aacaatgacg ctgttatttt tttcaacctt cgctccgacc gcgcccggca
    14941 gtttgcaaaa ctttttgtcc tggaaaaatc aaaagaaata aagctgccga aaccccgcct
    15001 caagaatttg tattttgtcg cgatgaccaa tttcggtccc gatctgccgg tccacaccgc
    15061 ttttcaggac catccggtca atggtacttt gccgacagcc ctgggagatt tccgccaact
    15121 ctatatcgcg gaaacggaaa aattcgcgca catcacctat tttttgaacg gaggctatgc
    15181 ggatgccatt gacggcgaag agcgcctgat gattccttca cccatcactg acaattatgc
    15241 caagctgcct caaatgtcag ccgaaaaaat cacgcaaaaa attttgggat ttctgcgaaa
    15301 aaataaatat gactttacgg ccgtcaactt tgccaacgct gatatggtcg ggcatacggg
    15361 caatatgaaa gcaacaatta aggcggcaga agttgttgac aagtgcctgg gaaaaatatt
    15421 taaagagctt caaagacaca atggcaatct gattgtcacg gccgaccatg gaaatgcgga
    15481 ttgcatgtgg gataagcgtg tcaatctccc aatgaccttc cataccaaaa atcccgtgcc
    15541 tctgatcatt gcctcggatg tttgtaaaaa aaagaagcta gcctcaggcg gagtactggg
    15601 gaatatttct ccaacgattt gtgatataat gaggacagag aagttgaaag agatgtcaaa
    15661 aaaaagctta ttataatttt tatattatgt acgatttaat atcgataggt tcaatcacga
    15721 gggacatttt tgttccgaca gaccgcggaa aaattttcaa gacaccaaaa gacaagctgg
    15781 ctcccgtctg gatcggtttt gaattgggag aaaaaatttg catagatgaa gtgattgaaa
    15841 ctggcggagg cgtcgccacc aatctgtcaa ttgggacaaa aaaacttggg ctgaaatctg
    15901 cgccactcgg ccccgcaaaa ccggcaactt ccgtaattct cattgaccaa aagacgggag
    15961 aacgagttat tttctatcaa aaatcttccg gaacaatcaa tattgataag ctaagcaaga
    16021 taaagacagg atggttgtca atttcgtctt tgactgggaa atggacaaag caagccggtc
    16081 aaatatcgaa ttatgccgcc aaaaacaaaa ccggactgat tcttttgcct agcactagcc
    16141 aaattcggga tgattttccg gatctcaaga aactgttgaa aacagccaaa attttgatat
    16201 tgaacaaaaa tgaagctctc gaaattgcat cgaaaatgaa aataaaagcg aataatacag
    16261 aaaatttatt caaactactc cacaagctcg gcccggaaat tatttgcatc actgacggaa
    16321 caaaaggcgc gtgggcgagc gacgaaaaaa atattttcca ttctccgatc caaaaagtga
    16381 aagcggttga tgtgacagga gcaggtgatg cttttgccag cggatttttg ggattttatc
    16441 tcaaaggcgg caacttgaaa accagtctcc gcgccggaat cttcaacagc gcaagcgttg
    16501 tgaagtatat tggcacaacc aaaggattgt tggcaaaaaa agacatttta gcaaaaataa
    16561 aatgaataaa ttggatcatt ttttctctcc aaaatcaatt gccattgtcg gcgcgtcacc
    16621 caaaagagga aaactgggca atgttttgat tgaaaacatc aaaaaaggcg gatggaaagg
    16681 aaaattgtat tttgtcaatc caaaatacgc gaaatctcgc ataggctatt tttcaaactt
    16741 aagtgaaata aaaaacccgg tggatctggt tttggttgct attcccgcgc cattcgcggg
    16801 cgaaattctt gaaaacggcg ccaaggcaaa gccggccatc aaaaattttg ccgtgatttc
    16861 ttcaggattc aaagaagctg gaaaagaggg cgcaatactt gaaaaaaatc ttgcaaatat
    16921 cgcgcaaaaa tataacctta atgttcttgg gccaaattgc ctcggctttt ccaatcctgc
    16981 cgaaaaattg aatgcgactt tcacggacgt gaagttgcaa ggggggagca tcgccattgt
    17041 ttcccaatcc ggcgcgctcg cggtcgcact gcttgactgg gcggaaagtg caaagattgg
    17101 tttctcaaaa atagtttcca tcggcaacaa agcggatctt gaagaaagtg agattctgaa
    17161 ttttctctca agcgacaaaa atacaagagc aatcgccctc tatcttgaag atatcaaaaa
    17221 cggaccgagg ttcatggagg cgctggcaaa aatcactcct cgaaagccgg tggtcgttct
    17281 caaggccgga aaaacagccg tcggacagaa ggctgtttca tcgcataccg gctcgcttgc
    17341 ccaggatgaa gagatcattg aagctgtttt tgaaaagacg aatgtcgtcc cggcgaacaa
    17401 tattgaagaa ttccaggata ttatatatta tctgaattcc aatcctgttc ccgcaaaaaa
    17461 tgaaatgata atattgacca atgccggcgg accgggcgtg atggcctctg atttcatcgg
    17521 aaaatcgaag agtataaaat tgcttgattt tcccgaaaaa ttcaaaaatg aaatcaaaaa
    17581 atatctgccg acgagcgctt ccgtcaacaa tccaattgac gtgatcggcg acgcgccgcc
    17641 cgaaagatac gcgaaaaccc ttgagcatat cgcaaaatat tttcccaaga atcctgtttt
    17701 agtcattctc acaccccaaa atcaaactga tccgaaaaaa gtcgctcaaa tattggtgaa
    17761 attcaggagt aaaattgaaa atatgtctat ttgcttcatg ggtggagaaa aaatagctcg
    17821 cgccaaagaa tttcttgcga aaaagaagat tcccaacttc gaaaacccgg aaagagcgtt
    17881 ggcggtgatt gaaaaattag cgaaatattc tcaatcgcaa agtaaagacg catttaatgc
    17941 gtcttcattg cacaacagac gcacgcagtg cctctctata aaaccaaact taataattca
    18001 aaaagctctc actgaaaaaa gaagaatcat tttctggaag gaagcaggaa aattgtttgg
    18061 gaaatttcag gtaaaattgg ccagatcaat ttctttcgaa agcatcattg agctcaaaaa
    18121 caaaaaaata cctttccctt gcgccctcaa aaccgatgat ccgaatatcg cccatcgtct
    18181 cgaaaaaaat gccgtaatgc tcgatatcca aaatgcgaaa gatctgaatg atactttcga
    18241 aaaaatgaaa aaagccaccg gcgtcaaaca ttttctcgtc caaacaatgg ctgatcccgg
    18301 cctcgagatg atcatcggaa tgaaaaatga tccaactttt ggtccggtca ttctttgcgg
    18361 ctggggcgga tcattcaccg agatattcaa agataaagtc atcttggttc ctccttttga
    18421 caaaaaagaa atagccaaca aactggagca gctggctatt ttccccatcc tcaagggatt
    18481 tcgaggcaaa aagggctatg atttggcaga aatagcaaaa gttattgctg ccactgccca
    18541 aatcgctttg gaaaatcccc aaattcggga aatcgacata aacccgctcg tggtctataa
    18601 tgacggaaaa gaagggaaga ttttggacgc taaaatattt ttaacataaa catatgaacg
    18661 agcaaaataa gttcaaaatt ctcatcgttg atgatgatga tattatccgc gaaacgtatc
    18721 gccaagtctt tcaagaacac gggtttgaat cgatcagcgc caaggacggg gttgagggaa
    18781 ttgacctggc aaccaaagaa aaattcgacg tgattctgac cggcatcatc atgcctcgga
    18841 tggacggatt tcagctcatt tccgctctgc gcgagaatgt ggataccaaa gatattccga
    18901 tggccatcat cagccatctc ggacgagaag aagatcgaag acgttccaag gaaatgggaa
    18961 ttgaaaattt cattgtccag ggaacaatga caccccgtga ggttgtcgac aaaatgaaaa
    19021 gtctcattgc ttccggcagc tcatttcagc ttggttttga ttattccgcc tgggacgcgc
    19081 cggctcttgc caaaaaaatg gaccttcccg gtttcgagtg ccccagctgc cagaacaaaa
    19141 tgatccttga actcacaccc aagtcggagg gaaaaggctt tgatgctttt ttcaaatgcc
    19201 caaaatgcga cctgcacccc
//
LOCUS       DATKGS010000121         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_35600, whole genome shotgun sequence.
ACCESSION   DATKGS010000121 DATKGS010000000
VERSION     DATKGS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35600"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VMQ48_03075"
     CDS             complement(<1..777)
                     /locus_tag="VMQ48_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HUD09043.1"
                     /translation="MDKEFKGKIEINSRYKLTKHSLPLIYTPGVAELSREIAKNKKRV
                     YDLTWKRNSVAIVTDGSAVLGLGNLGPEAALPVMEGKAVLFKEMGGVDAVPIVLDTQD
                     TEEIIKIAKAIAPGFGGINLEDISAPRCFEIEKRLIRELDIPVFHDDQHGTAIAVLAG
                     LVNALKVVKKDIKKIKIIISGAGAAGIAITKMLLKYGAKNIIAFDSRSPICRICSYNK
                     NDAKDELAKKTNLENFKGNLREAIVGADVFIGVSAPHLLKS"
     gene            complement(947..1873)
                     /locus_tag="VMQ48_03080"
     CDS             complement(947..1873)
                     /locus_tag="VMQ48_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUD09044.1"
                     /translation="MENNIYDLIIIGAGPAGLAASIYAARYKVNHLVIGKEPGGQAVE
                     AHKVENWPGTTSISGFELTQKMREHAEKLGAKLVMDSVSNISRSGDLFDVTAHTAQYK
                     AKNIILALGMEYKKLQIPGEEEFKGKGVSYCPTCDAAFFQDKVVVVAGGGNSAASAAQ
                     LLAEYASKVYVIYRGEKLKIDPSYLENFAKNERIKIIYNTNIKEIKGENIVEKIVLDK
                     DYDGKSELEAQGVFIEIGSEPGVELAKQLDANIDEQGFIVVGSDQGTNVSGIYAAGDA
                     TTGSNKMRQILTAAAEGAIAASSVYKSLQVSR"
     gene            complement(1876..>2079)
                     /locus_tag="VMQ48_03085"
     CDS             complement(1876..>2079)
                     /locus_tag="VMQ48_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUD09045.1"
                     /translation="RVIEELAEEVKDKYKVGKLNVDENKETASKYAVMSIPTLIIFKE
                     GKVVKQLVGVQAKENLKEELDKI"
BASE COUNT          495 a          536 c          383 g          665 t
ORIGIN      
        1 tgatttgagc aaatggggcg cggaaactcc aatgaagaca tccgcgccca caattgcttc
       61 acgcaaattt cccttgaagt tttccaagtt tgtttttttt gcaagttcat ctttggcatc
      121 gtttttgttg tatgagcata tgcggcaaat gggactgcgg ctgtcaaaag caatgatatt
      181 ctttgcgccg tatttcaaaa gcatctttgt gatcgcaatg ccggccgccc cggcgccgga
      241 aataattatt tttatttttt tgatatcttt tttcaccacc ttcagcgcgt tgacaaggcc
      301 tgccagcacc gcaattgctg ttccgtgctg gtcgtcgtga aatacgggaa tatccagttc
      361 ccggatcaat cttttttcaa tctcaaagca tcttggtgcg gaaatatctt cgagattgat
      421 cccgccaaat cccggcgcaa tcgctttcgc aatcttgatt atttcttcgg tatcctgcgt
      481 gtccaagact atcggtaccg cgtccacgcc acccatttct ttgaaaagaa ccgccttccc
      541 ctccatcacc ggaagtgccg cttccggacc aagatttccc aaacccaaaa cagcgctgcc
      601 atcagttaca atcgccacgc tgtttctttt ccaagtgagg tcgtatactc tttttttatt
      661 tttcgcgatc tcccgcgaaa gttcggccac accgggagta tatataagag gtaaagaatg
      721 tttggtgagt ttatatctgc tattaatttc tatttttcct ttaaattctt tatccatgtt
      781 ttcaatttcc aatttctaat tttcaatttc caaacaatga ctaaatttct caatgtctaa
      841 atgaaaaaat ttttaaaatt aattcattgg gtcattgaaa attgtttgaa aatttgtaaa
      901 ttgaaaattg aaaattatta ataagcaaca ctcaaaaaaa tgtcctttat ctcgaaactt
      961 gcaaactttt atatacactg ctggccgcga ttgctccttc tgctgccgcg gtgagaatct
     1021 ggcgcatctt gttcgagccg gtggttgcgt cgccggcggc gtatattccg gaaacattcg
     1081 tgccttgatc cgaacccacc acaatgaagc cttgctcgtc gatattcgca tcaagctgtt
     1141 tcgcaagctc aacccccggt tcactcccga tttcaatgaa gacgccctgc gcctccagct
     1201 cgctttttcc gtcataatcc ttgtccaaga caattttttc aactatgttt tcccccttta
     1261 tttccttgat attcgtattg tagatgatct tgattctttc gtttttcgca aaattttcaa
     1321 gatagcttgg atcgattttc aatttttccc cgcgatagat gacatatact tttgaagcat
     1381 actcggccaa aagctgcgct gccgacgccg cactgttccc gccgcccgca accactacaa
     1441 ccttgtcctg aaaaaaagcc gcgtcacaag tcgggcaata ggaaaccccc tttcccttga
     1501 attcctcttc acctggaatt tggagctttt tatattccat cccaagagcc aaaatgatat
     1561 tttttgcttt gtattgggct gtgtgcgccg tgacgtcaaa aagatctccg ctacgggata
     1621 tgttcgaaac ggaatccatg acaagtttcg cgcccagctt ttcggcatgc tcgcgcattt
     1681 tttgcgtgag ttcaaatccg gaaatggaag tcgttcccgg ccaattctca actttgtgcg
     1741 cttcaaccgc ctgccctccc ggttccttcc cgatcaccag atggttgacc ttgtaccgcg
     1801 cggcatatat cgaagcagcc aggccagccg gccccgcgcc aatgatgata agatcgtaaa
     1861 tattattttc cataattaga ttttatccag ttcttctttc aaattttctt tcgcttgcac
     1921 tccaacgagc tgtttcacaa cttttccctc tttaaaaatg atcagtgtcg gaatcgacat
     1981 aactgcgtat ttcgacgcag tttctttgtt ttcgtctaca ttcagcttgc caactttgta
     2041 cttatctttc acttcttccg ccaattcctc gatgaccct
//
LOCUS       DATKGS010000122        10090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_195822, whole genome shotgun sequence.
ACCESSION   DATKGS010000122 DATKGS010000000
VERSION     DATKGS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195822"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1724)
                     /gene="dnaA"
                     /locus_tag="VMQ48_03090"
     CDS             complement(333..1724)
                     /gene="dnaA"
                     /locus_tag="VMQ48_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HUD09046.1"
                     /translation="MANTEKMTNEDIWQAVLGEVELAVSPANFVTWFKNTSILSVEDG
                     RVNIRVPNGFAKEWLENKYNRYIITALRNFIDDVREISCSVVASPEVAPKKELDSAVP
                     ASAQVPNLGKISHESLSLRANLNPRYIFDNFVVGANNELAHAACEAVAKSPGSAYNPL
                     FIYGGVGLGKTHLLQSVGNALIEHNPDTKIIYATAEYFTNQYVDMVKNNQAHEFKRAY
                     INADLLLIDDIQFIGGKEKTQEEFFHVFNTLHQTNKQIVLTSDRPPKAIAMLEDRLRS
                     RFEGGMIADVSRPDIETRIAILQYKLSDKKTDIEIDNEVLSYIATNIQHNIRELEGAL
                     NRVIAACELSRSEPNLANVKKILGDIIHSGKKKGVTFKQILEGVAEYYDVATQDLVNR
                     CRRRDIVRPRQIAMFLMRAELQASYPGIGEQLGGRDHTTAMHAYEKISKELEQDEELE
                     QELNTIKSKIYAV"
     gene            1970..2107
                     /gene="rpmH"
                     /locus_tag="VMQ48_03095"
     CDS             1970..2107
                     /gene="rpmH"
                     /locus_tag="VMQ48_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HUD09047.1"
                     /translation="MPKRTYQPKKKRRATRHGFRKRMSTSDGQEVIKRRRRKGRKKLS
                     V"
     gene            2117..2464
                     /gene="rnpA"
                     /locus_tag="VMQ48_03100"
     CDS             2117..2464
                     /gene="rnpA"
                     /locus_tag="VMQ48_03100"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HUD09048.1"
                     /translation="MLPANHRLKGKKIFDDLFRLGKTFSNEALMMKVSKGEADQPTKF
                     GFGASLKFSKKAVERNLAKRWMRESVRMRIAEIEPGWNVIFFISPKFPKKELSLPLIR
                     EKTENLLKKAKIL"
     gene            2461..3186
                     /locus_tag="VMQ48_03105"
     CDS             2461..3186
                     /locus_tag="VMQ48_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HUD09049.1"
                     /translation="MTQLFHSIIYQPIYNALIFLYNVVPGHDLGVAIILLTIIIRLIL
                     YPLAKKQIESQKKMQELQPEIKKVQDKYKGDKEKQGKELMNLYKEKKVNPASGCLPLV
                     VQIIFIIALYRALVAGINFTECKDLYTFVACPAKIGTSFLGILDLAKPSIVLAVLTAA
                     AQFIQTKMMMGAVASAPSSGKGDFAQAMNKQMLYLGPLLTLFIGAKFPAGLPLYWLVM
                     TLFMIGQQYLVMGRETEKEKITS"
     gene            3200..3703
                     /locus_tag="VMQ48_03110"
     CDS             3200..3703
                     /locus_tag="VMQ48_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HUD09050.1"
                     /translation="MDSQEKEALLKKITAEVLQKMTFENFEISVASKNDAEGENFVIN
                     IETPESNLLIGQYGVTLAALQHTLRLLVRRQTEERFRFLADVNRYLQVKTDSLSELAI
                     ESAKQVIRDKKPVVLRPMSAYERRLVHLELAGNENVKTESIGEGEDRKVVIRPVGELE
                     KLDDKES"
     gene            3745..5193
                     /locus_tag="VMQ48_03115"
     CDS             3745..5193
                     /locus_tag="VMQ48_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HUD09051.1"
                     /translation="MSTPRKIAYNVAFSATAKMAGTVLALVGIGFITRYLGKDGFGDY
                     STILAFFFFFGAIGDLGLYAISTREISRPGSDEEEILGNVFSIRVASALLIVVISLFL
                     AYFLPYSSAIKTGIVISALAYVFSSGYSVLIGLFQKRLAMDKVAIGEFVGKIVQVAVI
                     VLAVKYNLGFQAIIWTLLLNMVVSFLIVFIWSRWYIKFYPAFDFTVWKKFLKESYPVG
                     ISAVIVFAYFKLDTILLSILKTSADVGIYNAAYKVLENISFFPAMFVGLVMPIMSRYI
                     FHERKKFELVADKTFKVFLVITVPLFIGTVFLAPSIIRLIGGAGFMEAVPVLRLLAAA
                     IIFIFFGNFFTNVIIAGNLQKKLMGILLFCAIFNVSLNLILIPRYSYIGAAVTSSLTE
                     GLVVLLSCFIAWKNLKYFPSFSKIVPILVSGAGMALVFFYFHFLNFFLLAAAGVFVYA
                     VLLWLFKAVSLDEVESLISKKGGEPTEFEPIA"
     gene            5198..6004
                     /locus_tag="VMQ48_03120"
     CDS             5198..6004
                     /locus_tag="VMQ48_03120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD09052.1"
                     /translation="MKLTIQIVNFRSRHYLEKCLFSIAENLPAILRTEVIIVNNDEKP
                     LDRAIADIGGGFAVQILETGKNIGFGRAHNAGFGRSQGDVVLFLNPDTRVLPGALQAL
                     LDVFEDQRIGIAGPLLVDSAGKIQPDCFGNIRTPFSTVGKKIFGRGAQPAGADGEIFE
                     TGWISGGAMLVRRDIFEKMGGFDENFFMYFEDVDLCLRVKKQGTLVAVNPKARIFHES
                     GKSFASEREKKAHYYTSQDYYMRKHFGPAAASLVKLLRLPYYVKNVYLKH"
     gene            6004..7281
                     /locus_tag="VMQ48_03125"
     CDS             6004..7281
                     /locus_tag="VMQ48_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUD09053.1"
                     /translation="MLNYLFLFLIFLLPLQFALNAGENIDLVTTRILVPVLFLAWMVK
                     SLARKKLWLPNKAEMWLVISFLFLASLSPYLGREASAGFRKLLYLFTIVPVFFVAADM
                     FREEKWRRRSIVAIIFSGALAAMVGLFQFLFSFLIGIDKSVKIWESLAPYFLGNSFGK
                     IVAANSSWLVNIGGVTRMRAFGFFPDPHNFAFFVNLCLFTALGYLFAQKKAVSKLWIG
                     AGAVLMIAAVGLSFSRGAYLGLLAGGVFFALIFLKRSGVLGKALVAIVAIFVLIFVFN
                     SNIITQRLATSFNLKEGSNAERYKNWTQATGMIQDYPLGGVGLGNYARTVDPTAADRS
                     SIYAHNLFLDIAAETGILNAIIFLILIFVSVWRNIRNNNMISLGVAAGLIAFLIHSIF
                     DTALYSPQVLTLLLIIIALGLNGNEKLKNQNGK"
     gene            7526..8491
                     /locus_tag="VMQ48_03130"
     CDS             7526..8491
                     /locus_tag="VMQ48_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUD09054.1"
                     /translation="MSVIANGNYYAGFGAIKGWFVVPIIFGIIFFDALKKDDSLLKKS
                     LLVLFFSGVAISIFGVVYKFLDVVTFDGRLKIFWDSPNQLAMFLAIPFLIGLFFALQK
                     QEVRAKRLYILSLTLVGLGLYFTYSYGAWLAMAVALIVIIWLKYSKKDHKKYLAIFAI
                     LLVAFLALASFFKYKSIENLGARSSLASRVTIWKSAGLMVEKNPLFGIGPGNFQEKYL
                     EYQKYFPPYLEWSAPQPHNIFLAFWLESGLLGLAGFVILLFYFFCDNKKTIENNRDLG
                     ILFLGIIIYILVHGLVDTTYWRNDLAVVFWVVVAMNICLAEKKQS"
     gene            complement(8488..9648)
                     /locus_tag="VMQ48_03135"
     CDS             complement(8488..9648)
                     /locus_tag="VMQ48_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09055.1"
                     /translation="MTKRIFAISTLVLVLLVGAIFVYNFAFKKPTVPTVAPKATDEGK
                     TSTTPAKTQDAAAQSSGAIVSVSDGGVFGATLAPDGNSLYVFSGDNGQLNQIDFNGKL
                     EKVVSTEEFQGLQQILWNKPRNKVIVKRADGSNKSRFLLLDLSQKTVTPVKDNVDSIT
                     WSGLGDKIIYKYYDPKTKKRTLEVSDPDGKNWRKIADINFVGLSISPVPGSSDISFWP
                     SPDAYTATAASLINFNGENKRDILKDRFGADLLWSPDGKYAAASSSDQKGGHKTDLFL
                     MNSDGGQFHSLNFFTFAKKCAWSDDSKFIFCAMPGNFPDSAILPNDWQAGTVQTADTF
                     WKIEVATGKKERLIDAAKVNGSFDALNPFLSQDEKTFFFTNKADGKLYKLGI"
     gene            complement(9748..>10090)
                     /locus_tag="VMQ48_03140"
     CDS             complement(9748..>10090)
                     /locus_tag="VMQ48_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HUD09056.1"
                     /translation="GDNPNSTNSPYRNQEKIPGAEPTSEFIPYLQSIINFGFAVIGIL
                     ALFMLIIGAYQYLMAAGSGNASDAKDTITSALLGLILGLCAWIILNTINPDLVKMNPI
                     TQIQGTNTSAK"
BASE COUNT         2461 a         2133 c         2507 g         2989 t
ORIGIN      
        1 gggggccaat aattttttca gctataagaa ttcccttttt taaattctcc gtagtgcaaa
       61 ttactttcat ggttatatta ttaatttaat tatttattta aaattgttgt tattattagg
      121 tttgggattt ttggggataa gttggttttg gatatatagt tattaattga tttgtttttg
      181 aaagttgtgt ataagggaga cgcgcggatg tggggagggg tgggataaaa aaatattttt
      241 ttcaatttgt ttttttatcc aaatgttgtc cactggctgg ctatatgttt tgaggcggct
      301 tatggataag ttgcccacaa gttgcgcgca gcttatacgg catagatttt acttttaatg
      361 gtgttgagtt cttgctcaag ttcttcatct tgctcaagtt ctttggaaat tttttcatag
      421 gcgtgcatcg cggtggtgtg gtctcgtccg ccgagttgct ccccaattcc gggataggag
      481 gcttgaagtt cggcccgcat caaaaacata gcgatctggc ggggacgcac tatgtcgcgt
      541 cgccggcagc ggttcacaag atcttgggtt gcgacgtcat agtattcagc cacgccttcg
      601 aggatctgtt tgaaggtgac gcctttcttt tttccggagt ggataatgtc accgaggatc
      661 tttttcacat tggcgaggtt cggctcggac cggctcaatt cgcaagcggc aataacgcgg
      721 ttgagcgccc cttccaactc gcggatattg tgctggatgt tggtggcgat atagctcaag
      781 acctcattgt cgatctcgat gtctgttttt ttgtcggaca atttgtattg caaaatggcg
      841 atcctggttt caatgtcggg ccgcgataca tccgcgatca ttccgccttc aaagcgggag
      901 cgcaagcggt cttcaagcat ggcaatggct ttgggcggcc ggtcgctagt gaggacgatt
      961 tgtttgttgg tttgatggag ggtattgaaa acgtgaaaaa attcttcctg ggtcttttcc
     1021 tttccgccga taaactgaat atcgtcgata aggaggaggt cggcattgat ataggcgcgt
     1081 ttgaactcgt gagcctggtt gtttttcacc atgtcaacat attggttggt gaaatattct
     1141 gctgtggcat agatgatttt tgtgtccgga ttgtgttcaa taagggcgtt tcccacggat
     1201 tggaggaggt gcgtcttccc aagtcccact ccgccataga tgaaaagcgg attgtaggcg
     1261 ctgccgggcg actttgcaac ggcttcgcag gcggcgtggg ccagttcgtt gttcgcaccg
     1321 acgacaaaat tgtcgaaaat atagcgcgga ttgaggttgg cgcgcaaact aagactttcg
     1381 tggcttattt tccccaagtt tgggacttgg gcggaggccg ggacggcgga atcgagctct
     1441 tttttgggag ccacttccgg gctggcgaca accgagcagg aaatttctcg cacatcatca
     1501 atgaaattgc gaagggcggt gatgatataa cggttgtatt tgttttcgag ccactccttg
     1561 gcaaatccgt ttggaacgcg gatattcacg cgtccgtctt caacggaaag aatggaggtg
     1621 tttttgaacc aagtgacaaa attggcgggc gaaaccgcca gctcaacttc gcccaaaacc
     1681 gcctgccaga tgtcctcatt cgtcattttt tctgtatttg ccataagtgg atctattata
     1741 tcacaagcca aaaatcggct ggcttggcga tgttgatagc cctggggata acctgttgac
     1801 agcataaaat attttgaaaa gaacagcaag tgattcaagt tgattggctg caaacgctat
     1861 gcattcctct ccccagcaat tataggtcga aaaatgaaca gaaaattgac cgttttggcc
     1921 aaagctgata caatcaagaa agtcaattag ctaaattatc gtcaaaatta tgccaaaaag
     1981 aacatatcaa cccaagaaaa aaagaagagc gacgcgacac ggatttcgga aacgcatgag
     2041 cacgagcgac ggacaagaag tgatcaagcg ccgccgccgc aaaggccgca aaaagttgtc
     2101 cgtttagttt tttgatatgc ttcccgcaaa tcatcgcctc aagggcaaaa aaatcttcga
     2161 cgacctcttc cggcttggga aaactttttc gaacgaggcg ttgatgatga aggtgtccaa
     2221 gggagaagcc gaccagccga ccaaattcgg cttcggggcg agcctcaagt tctccaaaaa
     2281 agcggtggag cgcaacttgg ccaaacgctg gatgcgcgaa tccgtgcgaa tgagaatcgc
     2341 agaaatagag ccgggatgga acgttatttt ttttattagc ccaaaatttc ccaaaaaaga
     2401 attaagcctt ccgctgatcc gggaaaaaac cgaaaatttg cttaagaaag ccaaaatatt
     2461 atgacccaat tgtttcactc catcatatac cagccgatct ataatgcgct gatcttcctt
     2521 tataatgttg ttcccggaca tgatctggga gtggcgatca tccttctcac gatcattatc
     2581 cggctgatcc tctatcccct tgccaaaaaa cagatcgagt cgcagaaaaa aatgcaggaa
     2641 ctgcagccgg aaataaaaaa agtgcaggac aaatacaagg gcgacaaaga gaagcaagga
     2701 aaagagctga tgaatctgta taaagaaaaa aaagtgaatc cggcttcggg ctgcctgccg
     2761 ctggttgttc aaatcatctt catcatcgcg ctttatcggg ccttggtcgc gggcataaac
     2821 ttcaccgaat gcaaagatct ctatactttt gttgcctgtc cggccaagat tgggacaagc
     2881 tttttgggga ttttggatct tgccaagccc agcattgttt tggcggtgct aacggcggcg
     2941 gctcaattca ttcaaacaaa aatgatgatg ggcgcagtgg ctagcgctcc ctcgagcggc
     3001 aaaggcgatt ttgcccaagc gatgaacaaa cagatgctct atctcgggcc gctcctcact
     3061 cttttcatcg gggcgaaatt tccggccgga ctccctctct attggctagt gatgaccctt
     3121 ttcatgatcg gacagcaata tttggtgatg ggacgcgaga cggaaaaaga aaaaataacc
     3181 agttgaatat ataaacacta tggacagcca agaaaaagaa gcgttgctca aaaaaatcac
     3241 ggccgaggtt cttcaaaaaa tgacttttga aaattttgaa ataagtgtcg cgagcaaaaa
     3301 cgacgcggag ggcgaaaatt ttgtcatcaa catcgaaaca ccggaatcaa accttttaat
     3361 cgggcaatac ggtgtgacgc tggcggcttt gcagcatacc ctgcgccttt tggtccgtcg
     3421 ccaaacagaa gaaaggttcc gatttttggc ggacgtgaac cgctatcttc aagtcaaaac
     3481 agattcgctc tcggagctgg cgatcgaatc ggcaaagcaa gtcatccggg acaaaaagcc
     3541 ggtggtcttg cgtccgatga gcgcctatga acggcggttg gtgcatttgg agctcgccgg
     3601 aaatgaaaac gtcaaaacgg aaagtattgg cgaaggagaa gatcgaaaag tggtgatccg
     3661 gcccgtcggt gagcttgaaa aacttgacga caaagaaagt taaaaaagtt tgcctttttc
     3721 gcttttagtt ttgaacttct tattgtgtct accccccgaa aaatcgctta taatgtagcc
     3781 ttttctgcca cagccaaaat ggccgggacg gtgctcgcgc tagtcgggat cgggttcatc
     3841 acccgctatt tgggaaaaga cggctttggg gactattcaa ccatccttgc ctttttcttt
     3901 ttctttggag cgatcggaga tttgggcctt tatgcaattt caacaaggga aatttcccgg
     3961 ccgggaagcg acgaagagga aattctcgga aatgtttttt cgatccgagt agcttccgcg
     4021 ctattgattg ttgttatttc tctttttctc gcgtattttc tcccttattc ctccgccatc
     4081 aaaacgggaa tagtcatctc ggctctggcc tatgtttttt cttcagggta ctcagtgctc
     4141 attggtcttt ttcagaagcg gctggcgatg gacaaggtgg cgatcggcga attcgttggc
     4201 aaaattgttc aggtggcggt gattgtgctc gcggtaaaat acaacttggg atttcaggca
     4261 atcatctgga cgcttcttct caatatggtg gtgagttttt tgatagtttt catctggtcg
     4321 cggtggtaca tcaaattcta tccggctttc gatttcacgg tttggaaaaa atttctcaaa
     4381 gaatcatatc cggtgggaat ttcagctgtc atcgtttttg cctacttcaa gcttgataca
     4441 attcttcttt ctattctcaa aaccagtgcc gatgtcggaa tatacaacgc ggcctacaaa
     4501 gtgctcgaaa atatttcttt ttttccggcg atgtttgtgg gcttggtgat gcccatcatg
     4561 tcgcgctata tttttcacga gcggaaaaag tttgagctgg tagctgacaa aacattcaag
     4621 gtctttcttg tgatcactgt tcctcttttc atcggcacgg tttttttggc gcccagcata
     4681 atccggctta tcggcggagc aggcttcatg gaagccgtac cggtgttgcg gcttctcgcc
     4741 gcggcgataa tcttcatatt ttttggaaat tttttcacca atgtgatcat tgccggaaac
     4801 ctccaaaaaa aattgatggg cattttgctt ttttgcgcga ttttcaatgt gtcgctcaac
     4861 ctcattttga tcccccgcta ttcctatatc ggcgcggcgg tcacttcaag tctcacagag
     4921 ggattggtgg ttctgctttc ctgcttcatt gcttggaaaa atctcaaata ttttccatcc
     4981 ttttcaaaga tagtcccaat tcttgtttcg ggcgcgggga tggctctggt ttttttctat
     5041 tttcatttcc tcaatttttt tcttctggca gcggccgggg ttttcgtcta tgcagtgctt
     5101 ctctggcttt tcaaggcggt gagtcttgat gaagtggaaa gcctcatcag caaaaaaggc
     5161 ggcgagccga cggaatttga acccattgct tgaatccatg aaacttacga tccaaattgt
     5221 taatttcaga agccggcact atcttgagaa gtgccttttt tcgatcgcgg aaaacctgcc
     5281 cgccattctt cgcacggagg ttataattgt caacaatgac gaaaaaccac tcgatagggc
     5341 tattgctgat attgggggcg ggtttgccgt ccaaattctt gaaacgggca agaacatcgg
     5401 tttcggaagg gcgcacaatg ccggctttgg ccggtcgcaa ggcgatgttg tccttttcct
     5461 caatccggat accagggttc ttcctggcgc cttgcaggcg cttctggacg tctttgaaga
     5521 tcagcggatt ggcattgccg ggccgctcct ggttgattcg gcgggaaaaa ttcagccgga
     5581 ttgttttgga aatatacgga ctcccttttc gacggtcgga aaaaaaatat ttggacgagg
     5641 cgcgcagccg gccggtgccg acggggaaat ctttgagacg ggttggatca gcggcggagc
     5701 aatgctggtg cgtcgggaca tttttgaaaa aatgggagga tttgatgaaa acttcttcat
     5761 gtattttgag gatgtggatc tgtgtcttcg ggtgaaaaaa cagggcacgc ttgtggcggt
     5821 caatccgaag gcgcgcattt ttcacgagtc cggcaagagt tttgcgagcg agcgggaaaa
     5881 gaaggctcac tattatactt cacaggatta ctatatgcgg aaacattttg gaccggccgc
     5941 ggccagtctt gtgaaattgc tacggcttcc gtattatgtg aagaatgttt atttgaagca
     6001 ctgatgctaa actatctttt cttatttcta atttttcttc ttcctctcca atttgcgttg
     6061 aacgcgggag agaatattga cttggtgacg acaagaattc ttgtgccggt tctttttctt
     6121 gcttggatgg tgaaaagttt ggcgcggaaa aaattgtggc tcccaaataa ggcggaaatg
     6181 tggttggtga tctcttttct tttcttggct tccctctcgc cttatctcgg gcgggaagcg
     6241 agcgccggtt ttcgcaagct tctctatctt tttacaatcg ttcccgtttt ttttgtggcg
     6301 gcagatatgt ttcgggaaga aaaatggcga aggcggagta ttgttgcaat tattttcagc
     6361 ggggctctgg cggcgatggt cggacttttc caatttctct tttcttttct catcggtatc
     6421 gataaatcgg tgaaaatttg ggaaagcctg gcgccatatt ttttgggaaa ttcgttcggg
     6481 aaaatcgtgg cggccaattc gagctggctc gtgaatattg gcggggtgac aaggatgcgt
     6541 gcctttggtt ttttccccga tccgcacaac ttcgcgtttt ttgtgaatct ctgtctattc
     6601 acagccctgg gatatctttt tgcccaaaaa aaagcagtat cgaaactttg gatcggcgct
     6661 ggagcggtct tgatgatcgc ggcggttggc ctgtcttttt caaggggcgc ctatctcggc
     6721 cttttggcgg gcggggtatt ttttgcgctg atctttctca agcgttcggg tgtgctcggc
     6781 aaagccttgg tggcgattgt ggcaattttt gttttgatat ttgtcttcaa ctcaaatatc
     6841 ataacgcaaa ggctagcaac atcgttcaac ttgaaagaag gatcgaacgc tgaacgctac
     6901 aaaaattgga cgcaagcgac gggaatgatc caggattatc ctctcggagg agtagggctg
     6961 ggcaactatg cccggacggt tgatccgacg gcggccgacc ggtcgtcgat ttatgcccat
     7021 aatttattcc tcgacattgc ggccgagact ggaattttga acgcgattat ttttctgata
     7081 cttatctttg tttctgtttg gcgaaatatc cggaacaata atatgataag tttaggtgtg
     7141 gctgcggggc taatcgcatt tctaatccac tcaattttcg acacagcgct ttattctccg
     7201 caagtgctga cgttgctttt aatcataatt gcgctagggc tgaacggtaa tgaaaaatta
     7261 aaaaatcaaa atggaaaatg acaatttaaa attaaaaatt gttttttggg atacaaattc
     7321 gcatatctct aaatttgttt tggtcacctt atttttgctt ccggtttatc ttgtcaaaat
     7381 aaaattcggc tggctgtctt ttaatgttct tgaattgttg atcataatcc ttttcctatt
     7441 ctgggttttc gataaaaata caaaatacac aatactaaat acaaaataca caatacctgt
     7501 cactcttata ttcgttggtc tcgttttgtc cgttatcgcc aatggcaatt attatgccgg
     7561 cttcggtgcg attaagggct ggtttgttgt tcctattatt tttggaatta ttttttttga
     7621 tgcgctgaaa aaagacgaca gtttgctaaa aaaatctttg ctcgttttgt ttttttccgg
     7681 cgtggcgatt tcaatcttcg gcgtagttta taaatttctt gacgtcgtaa ctttcgatgg
     7741 tcgcctcaag attttttggg attcgccgaa ccagctggcg atgtttttgg cgattccgtt
     7801 tttgattggg ttgttttttg cattgcaaaa acaagaagtg agggcaaaaa ggctatatat
     7861 tttaagttta actctagttg gattgggttt atattttaca tattcttacg gcgcgtggct
     7921 ggcgatggcg gtggcgttaa tcgtcataat ttggcttaag tatagcaaaa aagatcacaa
     7981 aaaatatttg gcaatttttg cgatactact ggttgctttt ctggctttgg caagtttttt
     8041 taaatataaa agcatagaga acttgggcgc tcgttcgtcg ctggcgtctc gggtgacgat
     8101 ctggaaatca gccggactga tggtcgaaaa gaatccgctt tttggaatcg ggccgggaaa
     8161 ttttcaggaa aaatatctgg aataccaaaa atattttcca ccctatctcg agtggtcggc
     8221 gccccaaccg cataatattt ttctcgcctt ttggcttgag tcggggctgc tcggtctcgc
     8281 gggatttgtt attttgcttt tttatttttt ttgcgacaac aaaaaaacga ttgaaaacaa
     8341 tcgcgatctg gggattttat ttttgggaat tataatctat atcctcgtcc acggcctagt
     8401 cgacacaacc tattggcgca atgacctggc ggtggtgttt tgggtggtgg tggcgatgaa
     8461 tatttgtcta gccgaaaaga agcaaagcta aattccaagt ttatacaatt tcccatccgc
     8521 cttgttcgtg aagaagaagg ttttttcgtc ttgggaaagg aaaggattga gcgcgtcaaa
     8581 agatccgttt accttggcgg catcaatcag ccgctctttc tttccagtcg cgacctcgat
     8641 tttccaaaaa gtgtcggcgg tttgaactgt gcccgcctgc cagtcattgg gaaggatcgc
     8701 cgagtcggga aaatttcccg gcatcgcgca gaaaatgaat tttgagtcgt ctgaccaagc
     8761 gcattttttc gcaaaggtga aaaaattgag agaatggaat tgccctccgt ccgaattcat
     8821 caaaaagaga tctgttttgt ggcctccctt ttggtcggag gaactcgccg cggcatattt
     8881 tccgtccggc gaccaaagaa gatcggcgcc gaaacgatcc ttcaggatat ctctcttgtt
     8941 ttctccgttg aagtttatca agcttgctgc cgttgccgtg taggcgtcgg gagacggcca
     9001 aaaagaaata tccgatgatc cgggaacagg gctgattgaa agcccgacaa agttgatatc
     9061 cgcgatcttg cgccaatttt ttccgtccgg atcggaaact tcaagggtgc gctttttcgt
     9121 tttcgggtcg tagtatttgt agattatttt gtctcccaat cccgaccagg tgatggaatc
     9181 aacgttgtct ttgacaggag tgacggtttt ttgcgaaaga tcgagaagca aaaacctcga
     9241 tttattggaa ccatccgccc gtttgacgat gactttgttt ctcggcttgt tccaaaggat
     9301 ttgctggaga ccctggaatt cttcggtcga aacaaccttt tccagtttgc cgttaaaatc
     9361 gatttggtta agctgtccgt tgtctccgga aaaaacatag agcgagtttc catccggggc
     9421 gagcgtggcg ccaaaaacac cgccatcaga aacggaaact atcgcgccgc ttgattgcgc
     9481 cgccgcgtct tgagtttttg cgggagtagt gcttgttttt ccttcgtcag tcgctttggg
     9541 ggcgacggtc ggcactgtcg gtttcttgaa ggcgaaatta tacacaaaaa tagctccgac
     9601 gaggaggacg agaacaagtg ttgagatggc gaatattctt ttggtcatta tttttgcaga
     9661 gagaaatttg tcattgtgct tttttcttca tcaaccagga cttcaattgt aaatcaatat
     9721 ccggatccgc cgttaacaga gcctgtgcta ctttgcgctc gtgtttgtcc cttgtatctg
     9781 cgtgatcgga ttcattttta ccaaatcagg attgatcgta ttcaaaatga tccatgcgca
     9841 gagtccgaga atgagtccca aaagtgccga ggtgatcgtg tctttcgcat ccgaggcgtt
     9901 ccctgatccg gccgccatca gatattgata cgcaccgatg atgagcatga acaaagccaa
     9961 aattccgata acggcaaacc cgaaattgat aatgctttgc agatacggga tgaactccga
    10021 agtaggctcc gcgccgggga tcttctcttg gtttctgtag ggactattgg tgctgtttgg
    10081 attatccccc
//
LOCUS       DATKGS010000123         6084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_136908, whole genome shotgun sequence.
ACCESSION   DATKGS010000123 DATKGS010000000
VERSION     DATKGS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136908"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VMQ48_03145"
     CDS             <1..670
                     /locus_tag="VMQ48_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUD09057.1"
                     /translation="ESTKKDLEEIYQVRKENIQVIPHGISQDFRVMEENDQRLDSTRK
                     KYELPQKFIFFLGNIEPRKNILSIIKAYKNIISSNPKLSEYKLVLAGNISPLCRDLIE
                     KEDIMTCGYIEREDRPYVYNLASLFVYPSFFEGFGLPVLEAMACGTPVIASNNSSLPE
                     VSGKSILSIDPNRPAELAEAIENILIDENLYNKFKKRGIAQAQKFSWEKCAKETLAVI
                     HQHK"
     gene            671..1792
                     /locus_tag="VMQ48_03150"
     CDS             671..1792
                     /locus_tag="VMQ48_03150"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUD09058.1"
                     /translation="MRIGIDARFFGSIGKGLGRYTQKLIENLESIDSENHYFIFLRRE
                     NWDEYQPRSQNFTKVLADVPWYTLREQVQMPKIFKRCGMDLVHFPHFNVPIAYKGKFV
                     VTIHDLILFRHPTRRASTLSAPAYFLKTKVYHRVIKRAIKNSEKIIAVSQHTKKDVLK
                     NFKINPDKVVVTYEGVDTFEKPLLEKPETVLKKYGIMRPYILYVGNAYPHKNLDRLIL
                     AFKEVSKTHSGLHLVLAGKEDYFYKRLKKFVVTNNVSGVVFPGHVAEDHLPALYREAR
                     LYVFPSLYEGFGLPPLEAMARNAPVVSSNASCLPEILGPAAYFFDPRGISETADAIEK
                     VLEDLDLRKYLVAAGREQIKKYSWQKMAKETLGIYKSCR"
     gene            1795..4320
                     /locus_tag="VMQ48_03155"
     CDS             1795..4320
                     /locus_tag="VMQ48_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HUD09059.1"
                     /translation="MSKRKNILPQMFDVKPVNSDGSLNFGKIKGLKRTVALPEKKITK
                     KNVSTKKSSLRLADVVGPKIYSKPKENKIKIAETRYDASPREEARFQPKPELFNKYFD
                     ASDETYQTKLKPDFDYFPTPENFSGRITRPEPEKKIRGKRARKFSLKKKIKKIRQFSG
                     RIIPDLGKLKIQENVSQLRISSASRFSYAFSFAVILLLFSCSIPAFSYVERVLDMKTN
                     LVATADMALGQFAQAKDNLSSSQFDKAAVDFSNSYQILSDANHDIQNIGGNFSEVLRF
                     VPGISKLASANYVLSAGEHFSLAGKSLAESAKSLNSIGNPLDSANQANQPSLTDLFLN
                     LRDGIQKSSGELSAAQDDLNKVNMDDLPPETRDQFVQLKSKLPLVNASLKNFLDYSQI
                     FLDVLGYNGQRKYLFLFENNQEMRPTGGFIGSYGILDIGNGRVKKLFVDDIYNPDGQL
                     KARVIPPTPIQKMSAVWTMHDANWFPDFPTSAEKVAWFYEKTGGPTVDGVIAITPDLL
                     QDLLKVTGPIDMPEYGKTIDADNFMEETQQEVEVDYDKELNQPKKFVADLTPKILDKV
                     MSERGIGSVLQTLKAFNNALSEKHLLLYSRNYNIQKLISDQKWSGEILNTDKDYLSVI
                     NTNINGYKTDGVIDETIDHKSEIQNDGSIVDTVSITRKHNGGNEKYDWWNKVNGDWMR
                     VYVPKDAKFLSVEGQTREIVPPALDYQSLGFKKDPQVQAEEDSTRVDDQSGTKIYDEN
                     NKTVFANWVYVSPGESATITYKYILPFKMRFDALQHPADSFSVLYQKQSGSVGSKLNS
                     QIDLPNSMQTIWHWPDNLNQDGNALKMESILDTDKFVGLAVEK"
     gene            4317..5873
                     /gene="murJ"
                     /locus_tag="VMQ48_03160"
     CDS             4317..5873
                     /gene="murJ"
                     /locus_tag="VMQ48_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HUD09060.1"
                     /translation="MIKSLQKKFLNSSPSSSVASAAFVIAMAGILSRVLGLFRDRILA
                     SHFGAGDTLDIYYAAFRVPDLVYNLLILGALSAAFIPVFTSLIAKDEEKEAWELVNVF
                     LTTALIFLIVIAAIFALFAPQLMKLITPGFSGDKLQGVAMLTRIMFLSPIFLGISGIF
                     GAVFLVKPFGIAGLAWGVVIGAFLHMVVQYPNVKMVGLKYFPRLSLKNRNFRKVITLM
                     IPRTMGLAVTQINLLVVTIIASTLAAGSISIFNFANNLQSFPIGIFAIPFAIAVFPIL
                     SHHAARDERDVFIHNFSKTFRQILFFIIPASILILVLRAQIVRIILGAGKFNWDDTIL
                     TFQALGIFAVSLFAQATIPLLARAFYALHNTRVPFLAGIGGEAMNLAAALILSRYYGI
                     LGLVWAFSLSSIVEMFLLIFILRNKLEYLDDKKIISVIFKISLSALAAGLIAQLAKYL
                     IEPYINLDTFLGVFWQMTFAGGLGVAVYFALAHFFNLEELHYLKKLFSTRIFRLRPPL
                     PEDPTEASGI"
BASE COUNT         1743 a         1409 c         1339 g         1593 t
ORIGIN      
        1 cgaatcaacg aaaaaagatc ttgaagaaat ctatcaagtg agaaaagaaa atatccaagt
       61 gatcccgcat ggcataagcc aagacttcag agtcatggag gaaaatgatc aaaggctgga
      121 ctcaacccga aaaaaatacg aactgccaca aaagttcatc ttttttcttg gcaacattga
      181 gccgagaaaa aacattttat cgatcatcaa agcctacaaa aatattattt caagcaatcc
      241 aaaactgtcg gaatataaac tggttttggc aggaaacatc agtccgcttt gcagggattt
      301 gatcgaaaaa gaagatatca tgacctgcgg atatattgaa agagaagacc gtccgtacgt
      361 gtataacctg gcttctcttt ttgtctatcc ctcatttttt gagggtttcg gcttgccggt
      421 tcttgaagcg atggcttgtg gtactccggt aatagcttcg aacaactctt cgctgccgga
      481 agtttccgga aaatccatcc tttcaattga cccaaatcgc ccggccgagc tcgccgaagc
      541 gatagaaaat attctcattg acgaaaactt atataataaa ttcaagaaaa gaggaatcgc
      601 gcaagctcaa aaattttctt gggaaaagtg cgcaaaagaa acattagctg tcattcatca
      661 acacaaataa atgcgaatcg gcattgacgc ccgctttttc ggatcaatcg gcaagggact
      721 cgggcgctat acacaaaaat tgatagaaaa tttggagagt attgattcag aaaatcacta
      781 tttcattttt ttgcgccggg aaaattggga tgaatatcag ccgcgcagcc aaaacttcac
      841 gaaagttctg gcggatgtcc cttggtacac cttgagagaa caagtgcaaa tgccgaaaat
      901 cttcaaaaga tgcgggatgg atcttgtcca ttttcctcat ttcaatgttc ccattgccta
      961 caaagggaaa tttgtcgtga cgatccatga tttgatcctg tttcgccatc caaccaggcg
     1021 cgcttccact ttgtcagctc cggcctattt cctcaaaaca aaagtatatc accgggtgat
     1081 caagagagct atcaagaact cggaaaaaat aattgccgtt tcgcagcaca caaaaaaaga
     1141 tgtgctgaaa aatttcaaga tcaatccgga caaagttgtc gtcacatatg aaggcgtgga
     1201 cacattcgaa aaaccattgc ttgaaaaacc ggaaacagtc ctcaaaaaat atggtataat
     1261 gaggccgtac atcctatatg tcggtaatgc ttatccgcac aagaatcttg accggctcat
     1321 tttggcgttc aaagaagttt ccaaaacgca ttccggtttg catttggtcc tggcggggaa
     1381 agaagactat ttctacaaac ggctgaaaaa atttgttgtg acaaacaatg tctcgggcgt
     1441 tgtcttcccg ggacacgtcg ctgaagatca tttgccggct ctttatcggg aagcaagact
     1501 ctatgttttt ccttcgctgt atgaaggctt cggattaccg ccgctggaag caatggcaag
     1561 gaacgcgccg gttgtcagct ccaatgcctc ctgtctgccg gaaattttgg ggccggccgc
     1621 ctatttcttt gatccgcggg gaatttccga aaccgctgac gcgatcgaaa aagttttgga
     1681 agatcttgat ttgcgcaaat atttggttgc cgcgggacgg gaacaaataa aaaaatatag
     1741 ctggcaaaaa atggccaaag agacactcgg aatatataaa agctgcagat aaaaatgtca
     1801 aaacgaaaaa acattctacc tcaaatgttt gatgtcaaac cggtaaactc ggatggttct
     1861 ttgaattttg ggaaaataaa gggattgaaa cggacagtcg ctcttcctga aaaaaaaata
     1921 accaagaaaa atgtttcgac aaagaaaagc agtttgcgtc tggctgatgt tgtcggaccg
     1981 aaaatatatt ccaaaccgaa agaaaataaa ataaagatcg cagaaacacg ctatgacgca
     2041 tctccgcgag aggaagctag gtttcagcca aaaccggagt tgtttaataa atattttgat
     2101 gcctctgatg agacatacca gacgaaactc aagccggatt ttgattattt tccaacgcct
     2161 gaaaattttt caggaaggat aaccaggccg gaaccggaaa agaaaattcg cggaaaaaga
     2221 gccagaaaat tttcgctgaa gaaaaagata aagaagatca ggcagttttc aggaagaatc
     2281 attccggatt tgggcaaatt gaaaattcaa gaaaatgttt ctcagctaag gatttcttcg
     2341 gcttcgcgct tcagctatgc tttttcattt gccgtcatct tgctcctgtt ttcctgttca
     2401 attcccgcct tttcctatgt ggagcgagtg cttgatatga aaacaaatct tgtcgcaacc
     2461 gccgatatgg ctcttggaca attcgcccag gctaaggaca atctctcaag cagccagttt
     2521 gacaaagcgg ccgtagattt ttccaattcc tatcaaattc tctccgacgc caatcatgat
     2581 attcaaaata ttggcggaaa cttcagtgag gttttgcgct tcgttcccgg aatttcaaaa
     2641 cttgccagcg ccaactatgt cctaagcgcc ggtgagcatt tttcactggc tggaaaaagt
     2701 ctggcggagt cggccaagtc actcaattcg atcggcaatc cgcttgatag cgcaaaccaa
     2761 gccaaccagc cttccctcac ggacttgttt ttgaatttgc gagacggaat tcaaaaatca
     2821 agcggggagc tttctgccgc tcaagatgat ttgaataaag tgaatatgga cgatctgccg
     2881 cctgaaacca gagaccaatt tgtccagctc aaaagcaagt tgcctctggt gaacgccagc
     2941 ctcaaaaatt tcctcgacta ttcccagata ttccttgatg ttttgggata caacggtcag
     3001 cgaaaatacc tgttcctttt tgaaaacaac caggaaatgc ggccaacagg cggcttcatc
     3061 ggaagctatg gaatccttga tatcgggaat ggccgcgtga aaaaactttt tgtggatgac
     3121 atttataatc cggatggaca gctcaaagca cgcgtgattc cgcccactcc gatccaaaaa
     3181 atgagtgctg tttggacaat gcacgacgcc aactggttcc ccgattttcc gacctcggct
     3241 gaaaaagtgg cctggttcta tgaaaaaacg ggcggaccga cggtggatgg agtgattgcg
     3301 atcactcccg atcttttgca ggatcttctc aaagtcaccg ggccgattga tatgccggaa
     3361 tacggaaaaa caatagacgc ggacaatttt atggaggaaa cccagcaaga agtcgaagtg
     3421 gactacgaca aagagctcaa ccagccgaaa aaatttgttg ccgacctcac gccaaaaatc
     3481 cttgacaagg tgatgagtga aaggggcatc ggctccgtcc tgcaaacact caaagccttc
     3541 aataacgctc tttcggaaaa acatttgctt ttgtattcga gaaattataa tatccaaaaa
     3601 ttgatctccg atcagaaatg gtcgggagag atcctgaata cagacaaaga ctatctgagt
     3661 gtcatcaaca cgaatatcaa cggctacaag accgacggcg tgatcgatga aacaattgat
     3721 cacaaaagcg aaatccaaaa cgacggaagc atcgttgaca cggtttccat tacgcgcaaa
     3781 cataacgggg gcaacgaaaa atatgactgg tggaataaag tgaacggaga ttggatgcgc
     3841 gtttatgtgc caaaagatgc aaaattcctt tccgtggaag gtcagacgcg cgaaatagtc
     3901 ccaccggcgc tagactatca atcgcttggc ttcaaaaaag atcctcaagt ccaggctgaa
     3961 gaagattcca cccgcgtcga cgaccaatcg ggaacaaaaa tatatgacga aaacaacaag
     4021 actgtttttg ccaactgggt ctatgtcagc ccgggagaat cggcaactat cacctataag
     4081 tatattttgc cgttcaaaat gagatttgac gcgcttcagc atccggctga ttctttttcc
     4141 gtcctctatc aaaaacaatc cggaagcgtc gggagcaagc tgaattccca aatcgatttg
     4201 ccaaacagta tgcaaactat ctggcattgg ccggacaatc ttaaccagga tggaaatgcg
     4261 ctcaaaatgg aatcgatttt ggatacggac aaatttgtag gactagccgt ggaaaaatga
     4321 tcaaatctct ccagaaaaaa tttttgaact cttctccatc gagctctgtg gcttcagcgg
     4381 cgtttgtgat tgccatggcc gggattttga gccgcgtctt ggggcttttt cgcgaccgta
     4441 tcctcgcttc gcacttcggg gcgggggata cactcgacat atactatgcc gccttccgcg
     4501 tgcctgattt ggtgtacaac ctcctcattc ttggagcctt gagcgccgct ttcattcctg
     4561 ttttcacgag tcttatcgcc aaagacgaag aaaaagaagc ctgggaactc gtgaatgtat
     4621 ttctcacgac ggcgctcatt tttctcattg tcatcgccgc aatttttgcc ctttttgccc
     4681 cacagctgat gaaacttatc accccgggat tttcagggga caaattgcaa ggcgttgcca
     4741 tgctgacacg catcatgttt ttgagcccga ttttccttgg catcagcgga attttcggcg
     4801 cggttttttt ggtgaagcct ttcgggattg caggactcgc ttggggagtg gtgattggag
     4861 cgtttctcca tatggtcgtc caatatccga atgtgaaaat ggtcggactg aaatattttc
     4921 cacgtctctc tcttaaaaat aggaattttc gaaaagtgat cacactgatg attccgcgca
     4981 caatgggact cgccgtcacc cagatcaatt tgctggtcgt cacgatcatt gcttcgactc
     5041 tcgccgccgg gagtatttcg attttcaatt tcgccaacaa cctgcaaagt tttccgatcg
     5101 gcatctttgc gattcctttt gccatcgctg tttttccgat tctttcccac cacgccgcgc
     5161 gcgatgaaag agatgtcttc atccataatt tttccaaaac tttccggcaa attttgtttt
     5221 tcattattcc ggccagcatt ttgatcctgg tcctgcgcgc ccaaatcgtg cgcatcatcc
     5281 ttggcgcggg aaaattcaat tgggacgaca cgatcctgac ctttcaagcg cttggaattt
     5341 ttgccgtgag ccttttcgct caagccacaa taccgctcct ggcccgggcg ttctatgccc
     5401 ttcacaacac ccgcgttcca tttcttgccg gcatcggcgg tgaagcgatg aaccttgcag
     5461 cagcgctcat tttgtcgcgc tattatggaa ttttgggact cgtctgggcg ttttccctct
     5521 cgagtatcgt agaaatgttt cttttgattt tcattcttcg caacaagctt gaatatcttg
     5581 acgacaaaaa aataatttcc gtcattttca aaatttctct ttccgcgctc gccgccggac
     5641 tgatcgccca actggcgaaa tatttgatcg aaccgtacat caacctcgac acgttccttg
     5701 gtgttttttg gcagatgacc ttcgcgggag ggttgggagt cgcagtatat tttgctctcg
     5761 cgcacttttt caacctcgaa gaactccatt atctcaaaaa acttttctcc acgcgaattt
     5821 tccgcctccg cccgcccctc cccgaagatc caacggaagc gagtgggatt tgaggcttgt
     5881 ataaggggaa tttcgcaggg gacttgactt tttggtgcga aaggtatata atacaaagtt
     5941 ggcaatgatc caacccacga atttaaaaaa attcaattag aggagactcg gaaatgaaga
     6001 ttcgcagatt ttttggtgct atccttttag tccttattgt cctgaccttt cttttgacaa
     6061 tctccgaata caaacccacc gcgg
//
LOCUS       DATKGS010000124         3251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_169051, whole genome shotgun sequence.
ACCESSION   DATKGS010000124 DATKGS010000000
VERSION     DATKGS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169051"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..560)
                     /gene="rplW"
                     /locus_tag="VMQ48_03165"
     CDS             complement(27..560)
                     /gene="rplW"
                     /locus_tag="VMQ48_03165"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HUD09061.1"
                     /translation="MSIFDKILGKSAQGGPASGGEDKLEKKSGKKTEKVEVEKKSADV
                     KSEKTTKVEKKIEKHVTKAAKPVKPKKEVVKRKVAKKDENIAHRVLLENLISEKSTLL
                     AAQNKYVFKVTKDSGKFQIKEAIEGYYGVQVVSVNTIKINPKKRIHGRTIGWKKGFKK
                     AVVTLRAGDTIGVAEGV"
     gene            complement(571..1218)
                     /gene="rplD"
                     /locus_tag="VMQ48_03170"
     CDS             complement(571..1218)
                     /gene="rplD"
                     /locus_tag="VMQ48_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HUD09062.1"
                     /translation="MIKFPVYNLKGEKVKEIELSEKVFGAKRNDALLHQVFVSQYANR
                     RQVLAHTKDRAERAGSGIKPWRQKGTGRARVGSVRTPIWRKGGIVFGPTKDRNFKKDV
                     PKKMGQKALAVVLSSKVKDAELFLVDSLVMKEAKTKIMSETIKNLKIKGSLLIGFSAK
                     EKEAKRASRNLPKVLNVEAKNLNVFDLLNHKYLLMSEEGAKILEKKFAAKKEIKS"
     gene            complement(1221..1787)
                     /gene="rplC"
                     /locus_tag="VMQ48_03175"
     CDS             complement(1221..1787)
                     /gene="rplC"
                     /locus_tag="VMQ48_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HUD09063.1"
                     /translation="MKFIFGKKLGMSTIYDEKQGALNVTLVECVPNAITQIRTEEKDG
                     YVAIQAGVKHEKKEKKFRKKKEFKSEAGELKVGDEMKIDQFEIGEKVKVSGVTKAKGF
                     QGVVKRHGFKGSMKTHGHKHDLRAPGSIGATYPEHVIKGKRMAGRMGGVRSSVLNLKI
                     VEIDKENNILFLKGAVPGVKGRIVEIRA"
     gene            complement(1939..2280)
                     /gene="rpsJ"
                     /locus_tag="VMQ48_03180"
     CDS             complement(1939..2280)
                     /gene="rpsJ"
                     /locus_tag="VMQ48_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HUD09064.1"
                     /translation="MATKKAEVQPEEAKSRIRIKIKAYDHKVIDRSARQIVETAERSG
                     AEIAGPVPLPTQIKKYTVNKSTFVHKDARDQYEMRIHKRLIDIFNPTPKTIDSLTNLD
                     LPAGVDIEIKM"
     gene            complement(2309..>3251)
                     /gene="tuf"
                     /locus_tag="VMQ48_03185"
     CDS             complement(2309..>3251)
                     /gene="tuf"
                     /locus_tag="VMQ48_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HUD09065.1"
                     /translation="HADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLAKQVGV
                     PAIVVFINKVDMVDDPELIDLVEAEVRELLTKYEFDGDNASVIRGSAVKALESKSKDD
                     EAAKPILDLIAAVDEKIPDPVRDIDKPYLMPIEDIFSIEGRGTVVTGRIERGIVNVND
                     EVEIVGIRPTVKTIVTGIEMFNKTLDKGQAGDNAGVLLRGTKKDDVERGQVLAKPGSI
                     TPHTEFDAEIYILTKEEGGRHTPFFKGYKPQFYIRTTDVTGDVTLPEGTEMVMPGDTV
                     NLGVKLMAPVAMEEGMRFAIREGGKTVGAGVVTKITK"
BASE COUNT          675 a          890 c          633 g         1053 t
ORIGIN      
        1 ggggagggac tggcatattt tttcaattaa actccttcgg ccactccgat tgtatcgcct
       61 gcccgaagcg tcaccacggc ttttttgaat ccctttttcc atccgatcgt ccggccgtga
      121 attctttttt tcggattgat cttaattgta ttcacgctca caacctgcac tccatagtat
      181 ccttcaatcg cttcttttat ctgaaacttt ccggaatcct tcgtcacctt gaaaacatat
      241 ttgttttgag cggccagcag agtcgatttt tccgaaatca aattttcaag aagaactctg
      301 tgggcgatat tttcgtcttt cttcgcgact ttccgtttca cgacttcttt cttgggtttt
      361 acgggttttg cagctttagt aacgtgcttt tcaattttct tttcaacttt agtagttttt
      421 tcgctcttaa catctgctga tttcttttca acttcaacct tttccgtttt tttcccggat
      481 tttttttcca atttgtcttc cccgcccgag gcgggtccgc cttgggcgga ttttcctaaa
      541 attttatcga aaatactcat aggtctcgga ttatgacttt atttctttct tggctgcaaa
      601 cttcttttct aaaatttttg ctccttcttc gctcatcagc aaatatttgt gattcaagag
      661 gtcaaaaaca ttcagattct ttgcctcaac attcaaaact ttcggaagat tgcggctagc
      721 ccgcttggct tccttttctt ttgcggagaa accgatcaac agactgccct tgattttcag
      781 atttttgatc gtttcgctca tgatttttgt ctttgcttcc ttcatcacga ggctatcgac
      841 caaaaacaat tccgcatcct tcacttttga actcaacacg acggccaaag ccttttgtcc
      901 catctttttc gggacgtctt tcttaaaatt ccgatctttc gtcggaccga aaacgattcc
      961 gcccttgcgc caaatcggcg tcctaaccga accaacccgc gcccggcctg ttcccttctg
     1021 ccgccatggt ttgattccgg atcccgcccg ttccgcgcgg tccttcgtat gggccaagac
     1081 ttgccggcga ttggcgtatt gggaaacaaa aacctggtgc aaaagagcgt catttctttt
     1141 cgcgccaaaa accttttccg aaagttcaat ctctttcact ttttcaccct tcaggttgta
     1201 tacgggaaac ttgatcatat ttatgctcta atttcaacga ttctgccttt cactcccggc
     1261 acggcgcctt tcaagaataa tatattattt tctttgtcga tttcaacgat cttgagattc
     1321 agcacgcttg accgcactcc gcccatccgg cctgccattc ttttcccttt gatgacgtgt
     1381 tccggatatg tagctccgat cgaacccggc gcgcgcaaat catgcttgtg tccgtgagtt
     1441 ttcattgatc ccttgaaacc atgccgcttc accactccct gaaatccttt ggctttcgtg
     1501 acgcccgaaa ctttcacttt ttcgccaatt tcaaactgat caatcttcat ctcatcgccg
     1561 actttcagct ctcctgcttc gctcttgaat tcctttttct tcctgaattt tttctccttt
     1621 ttttcgtgct tcactcccgc ctggatcgca acatatccgt ctttttcttc agttcggatc
     1681 tgcgtgatcg cattcggcac acattcaacc agcgtcacgt tcagcgcgcc ctgtttctca
     1741 tcatatatag tgctcattcc aagctttttc ccgaagataa atttcatcaa attttttcta
     1801 aggctaagcc ttagcagctg ctaaggctta gccttagaaa aactgctcat ttaaacaaaa
     1861 aactggttat aaaccagcca aaacccagaa ggattttatt gctagctcag tatcccgcga
     1921 attgggactt taactagatc acattttgat ctcaatatcc actcccgccg gcaaatcaag
     1981 atttgtgagg ctgtcgatcg ttttcggcgt cgggttgaaa atatcaatga gtctcttgtg
     2041 aatccgcatt tcatactgat cacgcgcatc cttgtgaacg aaggttgact tgttcacggt
     2101 atactttttg atctgggtag gcaaaggcac cgggcccgcg atttccgcgc ccgaacgctc
     2161 cgccgtttcc acaatctggc gcgcgctacg atcgatcact ttgtggtcgt aggctttgat
     2221 cttgatccgt atcctcgatt ttgcctcttc cggttgtact tccgctttct tggttgccat
     2281 ttgttaaggg atctgaaaaa attttaactt acttcgttat cttcgtcact actcccgcgc
     2341 cgacggtctt gccgccttcg cggatggcga atctcattcc ttcttccatt gcgaccggcg
     2401 ccatcaattt gactccgagg ttcaccgtat cgccaggcat aaccatctcc gttccttccg
     2461 gaagggtcac atcacccgtc acatccgtgg tccggatata gaactgcggc ttgtatccct
     2521 tgaagaacgg agtatggcgt ccaccttcct ctttggtgag gatatatatt tcggcgtcaa
     2581 attcagtatg cggagtaatc gaacccggct tcgcaagaac ttgccctctt tcgacatcat
     2641 cttttttcgt tccgcggaga aggactccgg cgttgtcccc agcttgtcct ttgtcgagag
     2701 ttttgttgaa catttcgatt ccggtgacga tcgttttgac cgtcgggcgg attcccacga
     2761 tttccacctc atcgttcaca ttaacaattc ctctctcgat tcttcccgtc accacggttc
     2821 cgcggccttc aattgaaaaa atgtcttcga ttggcatcag atacggcttg tcgatgtcgc
     2881 ggaccggatc cgggattttt tcgtccaccg cggcgatgag gtcaagaatg ggcttggccg
     2941 cttcgtcatc tttgctcttc gattcaaggg ccttgacagc tgaaccacga atcacggatg
     3001 cattgtcgcc gtcaaattca tatttggtca aaagttcacg cacttccgct tccaccaaat
     3061 caatcaattc cggatcatcc accatgtcga ctttattgat aaatacaacg atagccggca
     3121 cacccacttg ttttgcaaga agaatgtgtt cgcgggtctg cggcatcggt ccgtccgtcg
     3181 cagcaacaac caaaatcgct ccgtccattt gggcggcgcc ggtgatcatg tttttgatat
     3241 agtcagcgtg t
//
LOCUS       DATKGS010000125         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_196584, whole genome shotgun sequence.
ACCESSION   DATKGS010000125 DATKGS010000000
VERSION     DATKGS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196584"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="VMQ48_03190"
     CDS             <1..1829
                     /locus_tag="VMQ48_03190"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUD09066.1"
                     /translation="FFMTANELRQKYLDFFKEKGHAIIPSAPLVPENDPTVLFTTAGM
                     HPLVPFLMGEKHPLGTRLANVQKCVRTPDIDDVGDNRHLTFFEMMGNWSLGDYFKKEA
                     IEWSFEFLTSEKWLGLDPRRLYVTVFEGDDDAPLDQESINIWQEQFRKAGIEAKVAED
                     KIVNYEKRIVPLGKDDNWWGPAGETGPCGPDSEMFYDISPEDGAVEGKFEELNGPRLV
                     EIWNDVFMEYNKKADGTFEKLAQQNVDTGMGLERTLVTLTGKENVFETDIFQPLIRKI
                     EEISTREYKEEENQKAMRIISDHLRTATFMIADGVVPSNVERGYILRRLIRRAVRQGK
                     ILGIENNFAAKIAEVVISEYKETYPELAKENGKITEELNQEEVKFRETLEKGLKKIKE
                     IIAESRSAETISGEKAFYLFSTFGFPLELTKEIAREHSMAVDEEDFHEEFKKHQELSR
                     IVSAGMFKGGLADAGDKAARLHTATHLLLAALRKVLGKDVQQKGSNITGERLRLDFSH
                     SQKMSPDEIEKVEKLVNEAIEKNIPVKCAEMTLEEAQKSGAIGVFGDKYGEKVKVYSI
                     YDFSSEICGGPHAAATGELGKFKITKEEASSAGVRRIKAVLE"
     gene            1886..>2177
                     /locus_tag="VMQ48_03195"
     CDS             1886..>2177
                     /locus_tag="VMQ48_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09067.1"
                     /translation="MTGEGSALVRNAVSDEACVWTCWECPFFFKLVNEGNSPYSCLMV
                     KIVCHCEDDVEEEGGLCLFQDMAGPWVPVDVSRFTGSAALSTPCRRRYIGLPP"
BASE COUNT          631 a          439 c          609 g          498 t
ORIGIN      
        1 tattttttat gactgcaaac gaacttcgtc aaaaatatct cgattttttc aaggaaaagg
       61 ggcatgcgat cattccgtcg gcgcctttgg tgccggaaaa tgatccgacc gtgcttttca
      121 cgactgccgg gatgcatccc ctggtgccgt ttttgatggg cgaaaagcat ccactcggaa
      181 cacgtcttgc caatgtccaa aaatgcgtcc ggactcccga catcgacgac gtgggcgaca
      241 atcgacacct cacttttttt gaaatgatgg gaaattggtc gctaggggac tatttcaaaa
      301 aagaagccat tgagtggagc tttgagtttt taacgtctga aaaatggctt ggacttgatc
      361 cgcgccggct ttatgtgacg gtttttgaag gcgacgatga cgcgccgctt gaccaggagt
      421 cgatcaatat ctggcaggaa caattccgaa aagccgggat cgaagccaaa gtggccgaag
      481 acaaaatcgt aaattatgaa aaaaggattg tcccgcttgg aaaagacgac aactggtggg
      541 ggccggccgg agaaacaggg ccgtgcggac ccgacagcga gatgttctat gatatttctc
      601 cggaagacgg ggccgtggaa ggaaaattcg aagaactcaa tggtccgcgg ttggttgaga
      661 tatggaatga tgttttcatg gaatacaaca agaaagcaga cggaacgttt gaaaaattgg
      721 cgcagcaaaa tgtggatacg ggaatgggac tcgagcggac tctcgtgact ctcactggca
      781 aggaaaacgt ttttgagacg gatatttttc agccgctcat ccgaaaaatt gaagaaattt
      841 caacaagaga atacaaagaa gaagaaaacc agaaggcgat gcggatcatt tccgaccatt
      901 tgcggacggc gactttcatg attgcggatg gggtggtgcc gtcgaatgtc gagcgaggat
      961 atattttgcg ccggctcatc cggcgggcgg tccggcaggg aaaaattttg ggaatcgaaa
     1021 acaatttcgc ggccaaaatc gcggaagtgg tgatttccga atacaaagaa acctatcccg
     1081 agcttgcgaa agaaaatggc aaaatcaccg aagaattgaa ccaggaagaa gtgaaatttc
     1141 gcgaaacgct cgagaaaggg ctcaaaaaaa tcaaagaaat catcgcggaa agccggagcg
     1201 cggaaacaat ttccggtgaa aaagctttct atcttttttc cacgttcggt tttcctctgg
     1261 agctgacgaa agaaatcgcg cgggaacaca gtatggccgt ggatgaagaa gattttcacg
     1321 aagagttcaa gaagcatcag gaactttcgc ggatcgtttc ggccgggatg tttaagggcg
     1381 gcctggcgga cgcgggagac aaggcggcgc gcctgcatac cgccacccat ttgctcctgg
     1441 cggcgcttcg aaaagttttg ggaaaagatg tccagcaaaa aggatcaaac atcaccggcg
     1501 agcgcttgcg cctcgatttt tcgcatagtc aaaaaatgtc tcctgatgaa attgaaaaag
     1561 ttgaaaagct ggtgaatgag gcgatcgaaa aaaatattcc cgtgaaatgt gccgaaatga
     1621 cgctcgagga agcccaaaaa tcgggcgcga tcggcgtttt tggggataaa tatggggaaa
     1681 aagtgaaagt ctattcgata tacgatttct cgagtgagat ctgcggcggt ccgcacgcgg
     1741 cggcgacggg cgaactggga aaatttaaaa tcaccaaaga agaagcgagc tccgccgggg
     1801 taagaaggat caaagcggtg ttggaataag aatatttcaa tatataaaat agatcttata
     1861 caaaaaagaa ttgaggaggg cgattatgac aggagaggga tctgctttgg ttcgcaacgc
     1921 ggtttcggac gaagcatgtg tttggacttg ctgggaatgt ccattttttt tcaagctggt
     1981 gaatgagggc aattcaccat attcatgtct gatggtgaaa attgtatgcc attgcgagga
     2041 tgatgttgag gaagagggtg gactatgtct ctttcaggat atggctggtc catgggttcc
     2101 agtggatgtt tcccggttca ctggttcagc agctctctca actccatgca gaaggagata
     2161 tattggtctt cctcccc
//
LOCUS       DATKGS010000126         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_36864, whole genome shotgun sequence.
ACCESSION   DATKGS010000126 DATKGS010000000
VERSION     DATKGS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36864"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2371)
                     /locus_tag="VMQ48_03200"
     CDS             complement(212..2371)
                     /locus_tag="VMQ48_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUD09068.1"
                     /translation="MEIIKGLNEEQKEAVLATEGPVLVIAGAGSGKTRLLTHRVAYLI
                     REKGVNSRNILAVTFTNKAAGEMKERVKELLSGGYFREPWMGTFHHICIRILRVEIDK
                     IGYKKSFVIYDDSDQLALVKKIMKEKEVSTDQFNPRAMLAVISKAKGELQSVEDYITG
                     ESFFEGIVARIYEAYQKTLRDNNALDFDDILQKTVELFQKFPDILEKYQDIFRYILVD
                     EYQDTNKAQYVLVNLLAQRHRNLMVVGDDWQSIYSFRGADVRNILNFEKDYPEARVIR
                     LERNYRSTQKILDAAHGVISKNIHRKDKKLWTDNAGEHDIVVRKVSDEQEEAGFIVRE
                     IEGIMAKEKLSYNDFVVLYRTNAQSRAIEEAFLEATMPYRVIGGVRFYERREIKDFLA
                     YLKFIHNPSDEISLDRIINLPPRGIGAVTMGKWIRFTRENSLDFISSSREIKNMAGLN
                     SAKIEAIAKFGDMINRLREESQRVTLTNLMDSILEETGYRKLLLAEGEAGQIRYENVR
                     ELFTVAEKYKEEKNGLDIFLEEVALVAATDNIDSEAGAVHLMTMHAAKGLEFDTVFIA
                     GMEEGLFPHSRSLIDNHQMEEERRLAYVGITRAKKRIYLLWAVTRNIFGSVRVNVESR
                     FLDDIPEALKKEEKNDLSPFSKGRAGEGFNKYGKKKYPSNPPLRKGEDWKDGQRVRHN
                     AFGEGIVISTEGEIITIAFMKAGLKKLSTKFAELEKV"
     gene            2479..2748
                     /gene="rpsO"
                     /locus_tag="VMQ48_03205"
     CDS             2479..2748
                     /gene="rpsO"
                     /locus_tag="VMQ48_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HUD09069.1"
                     /translation="MTLTKQKKTKVIETSKTHEKDTGSPEVQISLFTEQIKKLTGHLK
                     KNKKDNHSRRGLLKMVSKRKKLMTYLKGKDAKRYKKLATNLGLKE"
     gene            2753..3256
                     /locus_tag="VMQ48_03210"
     CDS             2753..3256
                     /locus_tag="VMQ48_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HUD09070.1"
                     /translation="MPTGKFPEQRVGVLVDIQNMYYSAKVLYKKKVDFKEILKSGVAN
                     RKLIRAIGYGIKTIEGLEKNFFEALQKSGYEVRTKDLQIFPGGAKKGDWDVGITVDAI
                     KLAEHLDVIVLVTGDGDYIPLVEYLQNTKGCRVEVIAFAESTSAKLIEAADDFINLSE
                     NKKKFLI"
BASE COUNT          931 a          848 c          742 g          938 t
ORIGIN      
        1 tggggtcacg ttgctctcgt tgaatcggtg agtggatcaa gtttcactgt ttccgaaatg
       61 aattacgccg gtttcggaaa aaaagatttt cgtacgattt ccacgaacag tggtttcgtg
      121 agagggttta tatattagga aaaaaatatt aaaataatat tcttccatag agacgaggca
      181 ttgcctcgtc tctactttgt tataatattt cctaaacttt ttccaactct gcaaatttcg
      241 tggaaagttt tttgagaccc gctttcatga aagcgatggt gatgatctca ccttccgttg
      301 aaatgacaat accttctccg aaagcattgt ggcggacgcg ttggccatct ttccaatcct
      361 ccccctttcg taaaggggga ttagaggggt attttttttt gccatattta ttaaatccct
      421 ctccggctct ccctttagaa aagggagaga gatcgttttt ctcttctttt ttcaacgctt
      481 cgggaatatc gtcgagaaat cttgattcga cattcactcg taccgagccg aaaatattgc
      541 gcgtcaccgc ccaaagaaga taaatccttt ttttggcacg ggtgatcccg acataggcga
      601 ggcggcgttc ttcttccatc tgatgattat caatgagact gcgcgaatga gggaataggc
      661 cttcttccat tccagcgatg aaaaccgtat caaattcaag tcccttggcc gcgtgcatcg
      721 tcatgagatg caccgctccc gcttcggaat cgatattatc cgtcgcagcc acaagcgcta
      781 cttcttccaa aaaaatatct agtccgtttt tttcctcttt gtatttttcc gccaccgtga
      841 aaagctcgcg gacgttttca tagcgaattt gacccgcttc tccttcggcg agcagcaatt
      901 ttcgatatcc ggtttcttcc agaatcgaat ccatcagatt cgtgagcgta actcgctggg
      961 attcttcacg aagacgattg atcatatccc cgaattttgc aatcgcttcg attttcgccg
     1021 aattcaaacc ggccatgttt tttatctctc gtgacgaaga aataaagtcc aaactattct
     1081 ctctcgtgaa ccggatccac ttgcccatcg tcaccgctcc gatcccgcgc ggcggcagat
     1141 tgatgatccg atccaaactg atctcgtcgc tgggattgtg aataaatttg agataggcca
     1201 aaaaatcttt gatctcacgc cgttcataga atcgcacgcc cccgatgaca cggtagggca
     1261 tcgtcgcttc cagaaatgct tcttcaatgg cccgcgactg ggcgttggtg cgatacagca
     1321 cgacaaaatc attgtatgac aatttttctt tagccatgat tccttcgatt tcacggacga
     1381 tgaagcccgc ttcctcctgc tcgtcggaaa ccttgcggac cacgatatcg tgctcgcccg
     1441 cgttgtccgt ccaaagtttt ttgtctttgc gatggatatt tttggagatg accccatggg
     1501 ccgcatcgag aattttttgc gttgatcgat agttcctctc gagccggatg acccgcgcct
     1561 caggatagtc tttttcaaag ttcaaaatat tgcggacatc cgccccgcga aaagaataga
     1621 ttgactgcca atcatccccg accaccatca gattgcgatg gcgctgagcc agaagattca
     1681 ccagaacata ttgcgctttg ttcgtgtcct gatattcatc gacgaggatg tatcggaaaa
     1741 tatcctggta tttctcaaga atatcgggaa atttttggaa aagctcaact gtcttttgca
     1801 aaatatcgtc aaagtcaaga gcgttgttgt ctcgcagtgt tttttggtag gcctcgtaga
     1861 tccgcgcgac aatcccttca aagaaacttt ccccggttat ataatcctcg acgctttgca
     1921 actctccttt ggcctttgag atgaccgcga gcatcgctcg cgggttgaac tggtcagtgc
     1981 tgacctcttt ttccttcatt attttcttca cgagcgccaa ttggtcagaa tcgtcgtata
     2041 tcacaaaact ttttttgtag ccgattttgt cgatttccac gcgcaaaata cggatgcaga
     2101 tatgatggaa cgtccccatc cagggctcgc gaaaataccc gccagagaga agctctttca
     2161 ctcgctcttt catttcgccg gccgctttgt tggtgaaagt gacggccaaa atattgcggg
     2221 aattcactcc tttttccctg atcaaatagg cgacccggtg cgtaagaagc cgcgttttgc
     2281 cgctacccgc ccccgcgatc accaaaaccg gcccttcagt cgcaagaacg gcttcttttt
     2341 gctcttcatt taatcccttg attatttcca taacataatg tatgatagtc tacttggaga
     2401 aaaatgtcat tccttgccaa ataatccatt tagcggtatt ataaagccag taaaataagc
     2461 actaagcata cacatcctat gactcttact aagcagaaaa aaaccaaggt gatcgaaaca
     2521 agcaaaactc atgaaaagga taccggctcc ccggaagtcc aaatttcgct tttcaccgag
     2581 cagatcaaaa aacttaccgg ccacctcaaa aaaaacaaga aagacaacca ttcccgccgc
     2641 ggccttctta agatggtctc gaagcggaaa aaactgatga cctatctcaa gggaaaagac
     2701 gccaagcgat acaaaaaact ggccactaat ttgggactca aagaataatt ttatgccaac
     2761 aggcaaattt ccggaacaac gagtcggagt tttggtggac atccagaata tgtactattc
     2821 cgccaaagtc ctctacaaga aaaaggttga tttcaaagaa atattgaaat ccggggtagc
     2881 gaacagaaaa ttgatccgag ccatcggcta tggcatcaaa accatcgagg gcctcgagaa
     2941 gaattttttc gaagctttgc aaaaatccgg gtatgaagtc cgcacgaaag acctgcaaat
     3001 atttcccggc ggagcgaaga agggggactg ggatgtcggc atcacggtgg acgccatcaa
     3061 gctggccgaa cacctcgatg tgatcgtgct tgtcacggga gacggcgact atatcccgct
     3121 cgtcgaatat ctccagaaca ccaaaggctg ccgcgtcgaa gtgattgcct tcgccgaatc
     3181 gacctcggcg aaactgatcg aagccgcgga tgatttcatc aatttgagcg agaacaagaa
     3241 aaagtttttg atataatcca aaataattta acaataattc cgccggctct aatagccaat
     3301 cgcacacaaa taatactaaa ttttggtgtt agctgtgtgt cattcgtctt ttagcgctgc
     3361 gacggaaaaa ctcttcaatg gaagtgaaaa aatttgagac cgaaattgcc ggaagaaaac
     3421 tcgttgtcga aacgggtctt ttggcgcagc aagccaacg
//
LOCUS       DATKGS010000127         2754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_183141, whole genome shotgun sequence.
ACCESSION   DATKGS010000127 DATKGS010000000
VERSION     DATKGS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183141"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VMQ48_03215"
     CDS             complement(<1..246)
                     /locus_tag="VMQ48_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09071.1"
                     /translation="MKPNKIKKPKKSKKSKKSKKRKLDLFQEVCAKPNSAEIAELVLV
                     CAVVFLVSFLGIGKIFYDYSQENFKALNSQSEIQIVKS"
     gene            complement(300..2513)
                     /locus_tag="VMQ48_03220"
     CDS             complement(300..2513)
                     /locus_tag="VMQ48_03220"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HUD09072.1"
                     /translation="MTKNKVSLKISGMTCASCAVSNEKELLRAKGILSASVNFATKKA
                     YVEYDGDILSEEDVRKVIEKNGYGIEREDRNSTEQAEHENKIENSEHKHGDRDIQKNW
                     RAFLLSAVLSLPLVLEMFFKIRAGKDFLGINLAVWSHLILATVVVFWFGWRFHRMAYL
                     QAKKLRANMDTLVSLGTLAAYFFSLWAIFSGREGYLESAALIITLILLGKYFEAKSTG
                     RAGEAMRKLMELGVKKARVIKDGREEEVNIFEVKVGDIVSVRPGEKIPLDGIVLEGES
                     NVDESMLTGESLPVEKKKYSSVFGATVNQDGLLKIKVTKVGEGTVLAQIVKTVEDAQA
                     SKAPIQKLADKISGIFVPVIILIALATFLGWHFSGHALSAALINAVSVLVIACPCALG
                     LATPTAIMVGTGRGAKSGILFKSGESFERAKDISMVVFDKTGTLTKGKPEVISNIQNP
                     ISKISEEKLFKIAGSIAKYSEHPLSKAIAEYVRENNIETVEVDNIREMRGMGIVGECA
                     EHKAKLLLGNKKLLEENNIDIQWADEILNDEKSGVGTRLFVAHESEVVGAIIVADEIR
                     DEAKGVIQDLKNMGLKIAMISGDNKKTAEAVAEQLGIEKVLAEVLPSGKAAEIKNLQK
                     LGEKIIFVGDGINDAPSLVQADLGIAMGSAQDIAKEAGQIILVQNNLQKVVEAVKISK
                     LTFKTIRQNLFWAFGYNVIAIPLAAAGFLNPVIAAAAMAFSSVSVVANSLRIYRK"
     gene            complement(2532..>2754)
                     /locus_tag="VMQ48_03225"
     CDS             complement(2532..>2754)
                     /locus_tag="VMQ48_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09073.1"
                     /translation="EPSVFTVKQGVPVKWVINGVNVTGCTSTIIVPSHNISKSLKTGE
                     NIVEFTPQSKSVINFSCGMGMVRGKFIVE"
BASE COUNT          687 a          733 c          506 g          828 t
ORIGIN      
        1 gcttttcaca atttgtatct ccgactggct gtttaaggct ttgaaatttt cctggctata
       61 atcgtaaaag atttttccga tgcccaaaaa agaaaccaaa aaaaccacag cgcaaaccaa
      121 aaccagctct gcaatctcgg cgctgttcgg cttggcacag acctcttgaa aaagatccaa
      181 ttttcttttc ttggattttt tcgatttttt cgattttttt ggtttcttta ttttattggg
      241 tttcatattt ccagcagttc tggatgaatt atagcaaaaa ttttcaattt tgctaaattc
      301 tatttccgat atatcctcaa gctgttcgcc acaaccgaaa cggaactgaa agccatagcg
      361 gcggcagcga tgacgggatt caaaaacccg gccgcggcca aaggaatggc aatgacattg
      421 tatccgaacg cccagaaaag attctgtcga atcgttttga aagtcagttt ggatattttg
      481 accgcttcga ccactttttg aagattgttt tggacgagaa taatctgccc cgcttctttg
      541 gcgatgtcct gggcgctccc catcgcgatc ccaagatcgg cttggacaag gcttggcgcg
      601 tcgttgatcc catcaccgac aaaaataatc ttttctccca gcttttgaag attttttatt
      661 tccgccgctt ttcccgaagg caaaacttcc gccaaaactt tttcgatgcc aagttgttcc
      721 gccaccgcct cagcagtttt tttattgtcg ccggatatca tcgcaatctt gagtcccata
      781 tttttcaaat cctggatcac gccctttgct tcatcccgaa tttcatccgc gacaataatc
      841 gcaccgacca cttccgattc gtgcgcgaca aacaagcgcg tgccaacgcc cgatttttcg
      901 tcgttcaata tttcatccgc ccattgaata tcaatattat tttcttcaag caattttttg
      961 ttcccaagaa gaagctttgc tttgtgttcc gcgcactccc cgactattcc cattccccgc
     1021 atttctctga tattatccac ttcaacagtt tcaatattat tttcccggac atattcagct
     1081 atagccttgg acaaaggatg ttcggaatat tttgcgatac tccccgcaat cttaaacaat
     1141 ttttcctccg aaattttgga tattggattt tggatattgg atattacttc gggttttccc
     1201 ttcgtgagtg ttcccgtttt gtcgaaaaca accatcgaaa tatcctttgc ccgctcaaaa
     1261 ctttccccgc tcttgaaaag tattccgctt ttggctccgc gtcccgttcc aaccatgatc
     1321 gccgtcggcg tcgcaaggcc cagcgcgcat gggcacgcga tgaccaaaac cgagactgcg
     1381 ttgatcagcg ccgctgaaag cgcgtgtcca gaaaaatgcc agcccagaaa agtggcaaga
     1441 gcaatgagta tgatcaccgg cacaaaaatt cccgaaattt tgtcggccag tttttggatc
     1501 ggtgctttcg aggcctgcgc gtcttcaact gttttcacga tttgcgcaag aacggttcct
     1561 tcgccgacct tggtcacttt tatcttgagc agtccgtcct gattgaccgt cgcgccaaaa
     1621 acggatgaat attttttctt ctcgaccgga agcgattcgc ccgtgagcat tgactcgtca
     1681 acatttgatt caccctcaag cacaatcccg tcgagcggaa ttttttctcc cggccggaca
     1741 gaaacaatgt ctcctacttt cacttcaaaa atattcacct cttcttcgcg gccgtctttg
     1801 atcacccgcg cttttttcac cccgagctcc atcaattttc gcattgcctc tccggctctt
     1861 cccgtacttt tcgcttcaaa atattttccc agcaggatga gcgtgatgat gagcgccgcc
     1921 gattcgagat agccttcgcg gccggaaaat atcgcccata gactgaaaaa ataggcagcc
     1981 agcgttccaa gcgaaaccag tgtatccata ttggcccgca attttttggc ttggagatag
     2041 gccatccgat gaaaacgcca accaaaccaa aaaacgacaa ctgtcgcaag aatcaaatgc
     2101 gaccacacag caagattgat acccagaaaa tcttttccgg ctctgatttt gaaaaacatc
     2161 tccaaaacaa gtggcagact caaaaccgcc gagagcaaaa acgctcgcca atttttttgg
     2221 atatctctgt cgccatgttt gtgttcacta ttttcaatct tattttcatg ctctgcttgt
     2281 tcggtgctat ttcgatcttc cctctctata ccgtacccat ttttttcaat tactttcctc
     2341 acatcttcct cgctcaatat atctccgtca tattcaacat aagctttttt ggtcgcaaaa
     2401 ttaaccgacg cactcaaaat ccctttcgcg cgcaaaagtt ctttttcatt cgagaccgcg
     2461 caagaagcgc aggtcatacc ggaaattttg agagatacct tattttttgt catatatata
     2521 ttttttttat attattcaac aataaatttc ccgcgtacca ttcccatccc gcaggaaaaa
     2581 ttgatgacgc ttttgctttg cggcgtgaac tcaacaatat tttctcccgt cttcaaactt
     2641 tttgaaatat tatgcgaggg aacaatgatc gtactcgtgc agcctgtcac gttcaccccg
     2701 ttgatgaccc acttcactgg cacaccctgc ttcaccgtga aaacactcgg ttca
//
LOCUS       DATKGS010000128         4948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_196880, whole genome shotgun sequence.
ACCESSION   DATKGS010000128 DATKGS010000000
VERSION     DATKGS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196880"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VMQ48_03230"
     CDS             complement(<1..224)
                     /locus_tag="VMQ48_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09074.1"
                     /translation="MQMNKNWRSKFIDRAKRFFWHGFWTDPVIFFSIILAILANIGLW
                     IALFRTVTPTDSPIILHYNIYFGVDVVGNW"
     gene            complement(211..1272)
                     /locus_tag="VMQ48_03235"
     CDS             complement(211..1272)
                     /locus_tag="VMQ48_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HUD09075.1"
                     /translation="MSPNYFLFVFFVAIILVLTGLIFSLRKKIGDSKKDELENKDDRI
                     VNNLTNQISLLSQTLGTIQKSVDQRLGENTGRLDNAAKSYGEVQKQLTHLQDEVRRVH
                     EVGKDISSLHEILRAPKVRGVMGEIWLEKLLGQMLPQNHYECQYAFKSGEKVDAIIRL
                     QDNIISVDSKFPLENFKKAIESQNDEERSGFKKQFGQDVKKHIASISDKYILPGEGTM
                     DFALMYIPAENVYYEIVNHNFGNVSLFDYAVEKKVIPVSPNTFYAYLGTIIMGLKGLR
                     VEENAKFILQNLSKLMVQFMKIRKDFESLGSHIRNAQNKFEATDKRLGKFENQLEKSG
                     DKELEEIKNIVLDEVNADE"
     gene            complement(1295..1897)
                     /locus_tag="VMQ48_03240"
     CDS             complement(1295..1897)
                     /locus_tag="VMQ48_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09076.1"
                     /translation="MPEIFSQLFESRVKARLIKFFMLNDKREYSQQEVVQKNKMSSIQ
                     ITKELNRFEKMKMIIVRTKKGKRFFQTNTGFIFYPELKNLVVKSNTMPECRSLSQIKN
                     LGKVRLALISGVFLNYPKGRADLLLVGDEMSRAKLKHLLENLEAEVGKEINYSFMSSD
                     EFKYRTDMLDKFIMEFLESPYEEIICKVPNLKEIANRRAK"
     gene            2041..4131
                     /locus_tag="VMQ48_03245"
     CDS             2041..4131
                     /locus_tag="VMQ48_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09077.1"
                     /translation="MPISPKKRKNGYPKGLFREIFVSANRELAPFGMVFTYPPENFEQ
                     KQLGTVFGLIKISDSSPESSFVANLLASVIKKEYFAKPSRPAYDSFEASLKKANLALA
                     ELARQGSVKWIGHISFAGGALEKNNLHFSKLGTTSILLLRGGMLADIGSGLDEDTAQV
                     DSHPIKTFSDISSGKVELGDRLVFTTSDLLEIFSFEEIRQNAARFSREEFPEIISASL
                     TANSELSGAIVVSMVSEEEAEEQPVQLFTAKESKNRLIADLPEKQKSAAPSAIIPPKN
                     IPPASSPSPAHAYPAPASPIPGRPTAKKEKYLFVSESEDIIPKKSFAEKTTIIAKNTF
                     SGAKNGILLVWKKVYRLFGRVEWQKIRSGTKSLLASSSQKIRYIDFRKKNTKIGAVAG
                     IFVVLALIAFFLVRNVIRQNAAIKQAQQSIQNAQPSDMPAAEPSDLNAKNIENISDVA
                     ALTTDNNELIFMNGMLFSLTGDKSISKIDPASGAVEKSDSDIASGKFTLAAAMPDLNT
                     IFILTADKKVISFTPSNKKFQENNISFPDNLNASDIKAYLTYIYVLDSSANQIYRYPR
                     AEGGFGEKQDWLKNGQDLKGADKFAINNDIFAANNLNIIPLLQGKIDSAIDFQKPQIP
                     LRIDGIYTDRDFANILVLDNNNHRIVAFSKDGKIAAQYFSNQITGIKNMAVDEKNKVI
                     YLENSDSVSKFSIE"
     gene            complement(4203..4643)
                     /gene="rplL"
                     /locus_tag="VMQ48_03250"
     CDS             complement(4203..4643)
                     /gene="rplL"
                     /locus_tag="VMQ48_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HUD09078.1"
                     /translation="MTEEVKKEEVAVEEKKEVEVPKKFKSLVSEIEKMSVLELSELVK
                     VLEEKFGVSASAPMMMAAGPAAGGEAAEAAPEKSEFDIELVASGDSKINVIKAVREIT
                     GQGLKDAKDLVDAAPKVIKEKVAKAEAEDIKKKLEEAGATVNLK"
     gene            complement(4682..>4948)
                     /locus_tag="VMQ48_03255"
     CDS             complement(4682..>4948)
                     /locus_tag="VMQ48_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HUD09079.1"
                     /translation="GDEVSAAKVLAQFAKANKNFKILQGVLENKVISGKEVMSLAMLP
                     NKDQLLAKLVGTINAPISGFVNALAGNLKNLVGVLKAISEKPQV"
BASE COUNT         1336 a         1238 c          972 g         1402 t
ORIGIN      
        1 ttccaattcc ccaccacgtc cactccgaaa tagatattgt aatgcaaaat tatgggagaa
       61 tccgtcggcg tcaccgtacg gaaaagagca atccacaggc cgatattcgc caaaattgca
      121 agaataatgg aaaaaaatat caccggatcc gtccaaaatc cgtgccaaaa aaagcgcttg
      181 gccctgtcaa tgaattttga cctccagttt ttattcatct gcatttactt catctaaaac
      241 aatattcttg atctcttcga gctctttgtc gcctgatttt tcaagctggt tttcaaattt
      301 ccccaaccgc ttgtctgtgg cttcaaattt gttttgggcg ttccggatat gactgccaag
      361 cgactcgaaa tctttcctga tcttcataaa ttgaaccatg agcttgctca aattttgaag
      421 gatgaatttg gcattttctt caacccgcaa tcccttgagc cccataatga tcgtccccaa
      481 ataggcatag aaagtgttgg gggaaaccgg aatcactttt ttctcaaccg cataatcaaa
      541 aaggctcacg tttccgaaat tgtggttcac aatttcataa taaacatttt cggcgggaat
      601 atacatcagc gcgaaatcca tcgtgccttc tccaggcaat atatatttgt cggaaatgga
      661 ggcgatgtgt ttttttacgt cttgcccgaa ttgttttttg aaaccgcttc tttcctcatc
      721 gttttggctt tcaatcgcct ttttgaagtt ttcgagggga aatttcgaat caaccgagat
      781 gatattgtcc tggagccgga tgatcgcatc aactttctct ccgcttttga aggcgtactg
      841 gcattcgtag tggttctgcg gcagcatttg gccgagaagt ttctcgagcc agatctcacc
      901 catcacgccc cgcaccttgg gagcccgcaa gatctcatgg agcgatgaaa tgtcttttcc
      961 gacttcgtgg acccgccgaa cttcatcctg gagatgtgtc agctgctttt ggacttcacc
     1021 gtatgatttt gccgcgttgt cgagccgtcc cgtattttct cccagccgct gatcgacact
     1081 tttctggatc gtacccagag tttgtgacaa aagagaaatc tggttcgtca gattgttcac
     1141 aatccgatca tccttatttt ccaattcatc ctttttcgaa tcacctatct ttttgcgcaa
     1201 agaaaatata agcccggtga gaaccaggat tattgcaaca aaaaagacaa acaaaaagta
     1261 attaggcgac ataacaatgt tcaattatca attttcactt ggctctccgg ttcgcaattt
     1321 ccttcaaatt cggcactttg cagataattt cttcgtatgg gctttccaaa aattccatga
     1381 tgaatttgtc gagcatatct gttcggtatt tgaactcatc cgaactcata aatgaatagt
     1441 tgatctcttt tccaacctca gcctcaagat tttcgagtaa gtgtttgagc tttgcccggc
     1501 tcatttcatc cccgacaagc aaaagatcgg ctcgtccctt gggatagttc aagaaaacac
     1561 cggaaatgag cgccagtctc actttgccca aatttttaat ctggctgagg ctccggcatt
     1621 ccggcatagt gttcgatttt acaaccagat ttttgagctc gggatagaaa ataaaccccg
     1681 tgttggtttg aaaaaacctt tttccttttt tcgtccgcac gatgatcatc ttcatctttt
     1741 caaagcggtt gagctctttc gtgatctgga tggaactcat tttgtttttt tggacaacct
     1801 cctgttggga atattcgcgc ttgtcattca gcatgaaaaa tttgatgagc cgcgctttca
     1861 ctcttgattc aaaaagctgg gaaaatatct cgggcataaa aaaaatttag caagttagac
     1921 aatgccagta taaaccgaat gttggaaaaa gtccattttt gtagagacga ggcattgcct
     1981 cgtctctact tgataaattt gtatttttcc tcgttattcc ctataatgac ctcaagattt
     2041 atgcctatat cgcccaaaaa aagaaaaaat ggctacccaa aaggactttt tcgggaaatt
     2101 ttcgtttccg cgaaccggga attggccccc tttgggatgg ttttcacgta tccgcccgaa
     2161 aattttgaac aaaagcagct gggcacggtc tttggattga tcaagatttc cgacagctcg
     2221 ccggaatcgt cttttgtcgc caatcttctc gcatctgtga tcaaaaaaga atattttgca
     2281 aaaccctcgc ggccggccta tgacagcttt gaagccagcc tgaaaaaagc caacctcgct
     2341 ctcgccgaac ttgcccgcca aggcagtgtg aagtggatcg gacatatcag cttcgccgga
     2401 ggagccttgg agaaaaacaa tcttcatttt tcaaaattgg gaacgacttc catattgctt
     2461 ttgcgcggag gcatgctcgc cgacatcgga tccggcttgg atgaagatac cgcgcaggtt
     2521 gattcgcatc cgatcaaaac tttttccgat atctcaagcg gcaaagtcga gcttggcgac
     2581 cgccttgttt tcacgacatc ggacctgctt gaaatatttt cttttgagga aatccgccaa
     2641 aatgccgccc gtttttcccg agaagaattc cctgaaatta tttccgcctc cctcaccgcc
     2701 aactccgaac tctcgggagc aatcgtcgtg agtatggttt cggaggaaga ggctgaagag
     2761 cagccagttc aactcttcac agcgaaagaa agcaaaaatc gcctcatcgc tgatctgccg
     2821 gaaaagcaga aatcggctgc gccctccgca atcataccac ccaaaaacat tcctccggcc
     2881 agctctccat cgccagccca tgcttatcct gcgccagcct cgccgattcc aggcaggcca
     2941 actgccaaaa aagaaaaata cctctttgtt tctgaatctg aagatataat tcccaaaaaa
     3001 agcttcgcgg aaaaaaccac cattatcgca aagaatactt tttctggtgc aaaaaatggc
     3061 attcttctcg tctggaaaaa agtttatcgc ctttttggcc gggtcgaatg gcaaaaaatt
     3121 cgttcgggaa caaaatcatt gctagcgtcc tcatcgcaaa aaataagata tatagacttt
     3181 cgaaaaaaga acaccaaaat tggcgcagtt gccggaatat tcgttgttct cgcgctcatc
     3241 gccttttttc tcgtgaggaa cgttatccgg caaaatgcag ccatcaagca agctcaacaa
     3301 tccatccaaa acgcccaacc tagcgatatg ccggccgccg agccatccga cctcaacgca
     3361 aaaaacatcg aaaatatttc cgatgtcgcc gctttgacta cggataacaa cgagctgatc
     3421 tttatgaacg gcatgctttt ttcactcaca ggagacaaat ctatttcgaa aatcgatccc
     3481 gcaagcggag cagtggaaaa aagcgacagc gatattgcaa gcgggaaatt cacgcttgcg
     3541 gccgccatgc cggatctcaa cacgattttc attctcacgg ccgacaaaaa agtgatctct
     3601 ttcaccccct caaacaaaaa atttcaagaa aataatattt ctttccccga caatctgaac
     3661 gccagcgata taaaggcata tctgacctat atttatgttc tcgattcatc cgccaatcaa
     3721 atatatcgct accctcgcgc cgaaggcggc ttcggcgaaa agcaggattg gctgaaaaac
     3781 ggacaagatc tcaagggcgc agacaaattc gcgatcaaca atgatatctt tgcggccaac
     3841 aacctgaata tcattcccct tcttcaggga aaaattgaca gcgcgattga tttccaaaaa
     3901 ccacagattc ctcttcgcat cgatggcata tatacagaca gggatttcgc aaacatactc
     3961 gtcctcgaca acaacaacca ccggatcgta gcgttttcaa aagacggaaa aatcgccgcg
     4021 caatatttca gcaaccaaat aaccggcatc aaaaatatgg cggtggatga aaaaaacaaa
     4081 gttatatatc ttgaaaattc ggattccgtt tcaaagtttt cgatagaata aatggcactg
     4141 aattttatac aaaaaagacg ggaaacatcc cgtctttttt tctttggttc tatttttaaa
     4201 atttatttca agttcaccgt tgccccggct tcttccaatt tcttcttgat atcttccgct
     4261 tcagccttgg cgactttttc cttgatcacc ttcggagccg cgtccaccag atccttcgcg
     4321 tctttgaggc cttgtccggt gatttcgcgc accgctttga tcacattgat cttggaatcg
     4381 ccggatgcga cgagctcgat gtcaaattcg ctcttttcgg gagcggcttc cgcggcttcg
     4441 cctcccgctg ccggtccggc agccatcatc atcggagcgc tggcgctcac accgaatttt
     4501 tcctcgagaa ccttcaccag ttctgaaagt tcaagaactg acatcttctc gatttcgctc
     4561 acgagagatt tgaacttctt gggaacttcg acttcttttt tctcttcgac tgcaacttct
     4621 tcttttttta cttcttcagt cataaaattg aattaaatat ttataaatta tttctactga
     4681 tctagacttg tggtttttct gaaattgcct tgagtactcc gaccaaattc ttcaaatttc
     4741 cagccagcgc gttcacgaat ccggaaatcg gagcattgat cgtgccaacc agtttggcga
     4801 gcagctgatc cttgtttggc aacatcgcga gagacatcac ttctttgccg ctgatcacct
     4861 tgttttcaag aactccctgc aatattttga aatttttgtt ggctttcgcg aattgcgcca
     4921 gaactttggc tgccgacact tcatctcc
//
LOCUS       DATKGS010000129         4291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_22544, whole genome shotgun sequence.
ACCESSION   DATKGS010000129 DATKGS010000000
VERSION     DATKGS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22544"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..421)
                     /locus_tag="VMQ48_03260"
     CDS             complement(53..421)
                     /locus_tag="VMQ48_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09080.1"
                     /translation="MYQKLFSKIAKDLLTALIATYFILLIPELVLPGIVSSHFNPEYL
                     LIFILLLGLAYSKFGSKIKRPENPKFQAISRNLINIILFVIAIMLILSLYKMHIWEIV
                     VVIAISVPLLIGAEKILIKE"
     gene            complement(425..1411)
                     /locus_tag="VMQ48_03265"
     CDS             complement(425..1411)
                     /locus_tag="VMQ48_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09081.1"
                     /translation="MTEEKLEKIYRIIRVAIFLIPVLVVVVGAYLVFFPIDSYNFYPD
                     SPKLSKFYISKNADTNALSFGVFPLRTYRYVDLSMTFKKNDKANCDNAAPEVTLAKTY
                     QVFLDPTADAIAFEDQLRSLLFEDNKTKFPNGSLLHLKPTNEVFLITHGKKILFPGPE
                     ILLAFGYSFDNLIDVEQSDIDQFPNADQKVFLWTMPHPDGTIFQSFPSHTLYIVSDGK
                     KYPIASKDLLNSVWPENYAIPVSDPIPENTLKCDTKNASKGISCQFDSTKLSNIGGYY
                     LFSVNFPASCSIDNIHPGSAQIGFSSEHSVATIKDSLKNIAASALNRYFYKQ"
     gene            1500..2765
                     /locus_tag="VMQ48_03270"
     CDS             1500..2765
                     /locus_tag="VMQ48_03270"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HUD09082.1"
                     /translation="MGLFSKIIGRRNGTNFALSLDIGTEVVKALVFKIDEESGKGIVA
                     GVGRIRQKLGDMQSGAVSDISSVVSSCEKAIAVAEEMAGTRRVEQAIMGIAGELVKGS
                     TTTVHYERLKPETRIDLPELKNIVQKVQWKAFDRIRQQLAWETGHSEIDVKLINAAIV
                     DVRIDGYRVTNPLGFQGRDVSIGIFNAYAPMVHLGALQTIAEELDLDLLSIAAEPYAV
                     ARSMGMENTMDFSTIFIDIGGGTTDIAVVRNGGLEGTRMFALGGRAFTKRLAQELNIN
                     FEEAEMLKIRYGRGELNKEVVLRLEKIFEEDCRVWLDGVELSLSEYAENDLLPSRILL
                     CGGGSALPGIKKILTSPAWLKNLSFSKPPSVSFLQPKDVVNIEDTTGLLRDPQDITPM
                     GLANLALELVGEEKVLSSMLRRAVKMMQN"
     gene            2775..>4291
                     /locus_tag="VMQ48_03275"
     CDS             2775..>4291
                     /locus_tag="VMQ48_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09083.1"
                     /translation="MHKTLYIDIDEEITSIIDRMRKAEAREVIIVAPKNAMLLQSIVN
                     LKLLKKEADRRKKQLLIITQDKIGKKLIEKAGILAQAKPGKDLYLNAEPDEKSYDPMY
                     AAEASEIKEELEKEEQEKEIGSSDFFDQPLSPKESEIIDNESVLIPEPRQVGRKKAPQ
                     VASKKSARNEKLPSSSQFSGAAEKNEEKARVSMSDIVMNESQPKKKKPKPEVIEVEQE
                     PQSKADQSLAGEDKQGEKTRFSNSFSQAYAEANAVPGSVSRVSIKKADKFFRGSRKLK
                     KDLEIARVGGGAKKFFVFFAGIFVLLAALAATYFLFPKATLVLQIKNQEKSVSLDLTA
                     AAQNNATNIADKTLPANLEQITKEVTEDFDATGSKDGGAKATGQAVIYNEFSGENQPL
                     VATTRLETDDGKIFRITKNVVVPGMTNVGGETKPGAIEVDIAADQPGENYNIDPANFK
                     IPGFKDTPAKYEKFYAKSSNPTTGGSSGAAKVVTAQDIASAKEKIAVDGKKAAIA"
BASE COUNT         1266 a          845 c         1149 g         1031 t
ORIGIN      
        1 cgagagaaac gttggagtgg ataaagtcgt agagacgaga ttaatcgcgt ctctactctt
       61 ttatcaagat tttttccgct ccaataagaa gaggcacgga aatggcgatc acgaccacga
      121 tttcccagat gtgcatttta taaaggctca agatcagcat tatcgcgata acaaagagga
      181 tgatgttgat caaattgcgg gaaatggctt gaaattttgg attttcaggc ctttttattt
      241 tgctcccgaa cttcgaatag gccagtccta gaagaaggat gaagattaaa aggtattccg
      301 ggttgaaatg agagctcacg atgccaggca agaccaattc gggaataagc aagatgaaat
      361 aagtggcgat caaagcggtc agaaggtctt tcgctatttt tgaaaaaagt ttttgataca
      421 tgatttattg cttgtaaaaa tacctgttca gtgccgaagc ggcaatattt ttcaaagaat
      481 ctttgatggt tgcaaccgaa tgttcagaag aaaatccaat ctgagcggat ccagggtgaa
      541 tattgtcgat gctgcagctc gctggaaaat taacggagaa aagatagtag cccccgatgt
      601 tcgaaagttt tgtgctatca aactggcagg atattccttt ggaggcattt ttcgtatcgc
      661 atttcaacgt attttccggg atgggatcac tcacggggat agcatagttt tccggccaga
      721 ccgagttgag aagatctttg ctggcgatag gatacttttt cccgtcagaa acaatataaa
      781 gcgtatgtga tgggaaagac tggaaaattg ttccgtccgg atggggcata gtccacagaa
      841 agactttttg gtcggcattc gggaactggt cgatgtccga ttgttcaacg tcgatcaaat
      901 tgtcaaagct atatccgaaa gccagcagaa tttcaggtcc ggggaaaaga atcttttttc
      961 cgtgcgtgat gagaaaaact tcattcgtcg gtttgagatg aagcagggag ccatttggga
     1021 atttggtttt attgtcttca aagagaaggc tgcgcagctg gtcttcgaaa gctattgcgt
     1081 cagctgtcgg atcaagaaag acctgatagg tttttgccaa agtcacctcg ggtgcggcgt
     1141 tgtcgcagtt ggccttgtcg ttttttttga aggtcattga aaggtcgaca tagcgatagg
     1201 tgcgcaaagg aaagactcca aacgaaagag catttgtgtc cgcgttcttg gaaatgtaaa
     1261 atttggatag ttttgggctg tctggataaa aattgtatga gtctatcgga aaaaaaacca
     1321 agtaggctcc aacgactacg acaagtacgg ggatgagaaa aatcgcaacc ctgattattc
     1381 gataaatttt ttcgagtttt tcttcggtca tatgaactcc tttatattaa acaaagaaat
     1441 gccttgcgtc aaagggtgaa ttgtcaactt gtccgatttt gggtataatt gaaccagcta
     1501 tgggattatt ttccaaaatc atcggacgaa gaaacggaac caattttgcg ctttctcttg
     1561 atattgggac ggaggtcgtg aaagcgcttg ttttcaagat cgatgaagag tcggggaagg
     1621 gtattgtcgc gggcgttgga cggatccggc aaaagctggg ggatatgcaa tcgggagcag
     1681 tgagtgatat ttccagcgtg gtttcttctt gcgaaaaagc gattgcggtt gccgaagaaa
     1741 tggcggggac aagaagggtt gagcaggcta tcatgggaat tgccggcgag cttgtgaagg
     1801 gttccaccac gactgttcac tacgaaagat tgaaaccgga aactcgcatc gatctcccgg
     1861 agctcaaaaa tattgtccag aaagtacagt ggaaggcttt tgaccggatc cggcagcaac
     1921 tggcgtggga aacagggcac agcgagatcg atgtgaagct catcaacgcg gcgatcgtgg
     1981 atgtgcggat cgacggttat cgagtgacaa atcctttggg attccaaggt cgggacgttt
     2041 ccatcggaat tttcaatgct tatgccccga tggtccatct gggggctctt caaacaattg
     2101 ccgaagaatt ggacctggat cttttgagca tcgcggccga accgtatgcg gttgcccgct
     2161 cgatgggaat ggaaaacacc atggatttta gcacaatttt cattgacatt ggcggcggaa
     2221 caacggatat tgcggttgtg cgaaacggcg gtctggaagg gacaagaatg ttcgcgctcg
     2281 gcgggagagc tttcacgaaa agattggccc aggagctcaa catcaatttt gaggaggcgg
     2341 agatgctcaa aatcagatac ggccgaggag aattgaacaa agaggttgtc ttgcggctgg
     2401 aaaagatatt tgaagaggac tgccgggttt ggcttgacgg ggtggagctc tcgctttcag
     2461 aatacgccga aaatgatctt ttgccttcaa ggattttgct ttgcggtggc gggtcggcgc
     2521 ttccgggaat caaaaaaatc ctgacaagtc ccgcttggct caaaaatctt tctttttcaa
     2581 agccgccttc ggtgagtttt ttgcagccga aagatgttgt caatattgag gatacaactg
     2641 gtcttttgcg tgatccgcag gacatcactc cgatgggact ggcaaatttg gcgctcgagc
     2701 ttgtcggaga ggaaaaagtg ctgtcttcga tgcttagacg ggccgtgaaa atgatgcaaa
     2761 attaataaat ttccatgcat aaaaccctat atatcgacat cgacgaagag ataacctcca
     2821 ttattgaccg gatgcggaaa gcggaggcga gggaagttat catagtcgcg cccaaaaacg
     2881 cgatgcttct gcagagcatc gtcaatttga aattgctcaa aaaagaggct gaccgcagaa
     2941 aaaagcagct cttgatcatc acgcaggaca aaatcggaaa aaagctgatt gaaaaagcgg
     3001 gaattttggc gcaagcaaaa cccggaaaag atttgtatct caatgccgaa ccggatgaaa
     3061 agtcatacga tccgatgtat gcggcggagg cctctgaaat caaggaagaa ctggaaaaag
     3121 aagagcaaga aaaagaaatt ggctcaagcg acttttttga tcaaccgctg tcgccgaagg
     3181 aatcggaaat aattgataac gagtcagtat tgattccgga gccaaggcaa gttggaagga
     3241 aaaaagcgcc tcaagtagcg tcaaaaaagt ccgcgagaaa tgaaaaactt ccatccagca
     3301 gccaattcag tggagcggct gaaaaaaatg aggaaaaagc gcgtgtcagt atgtctgata
     3361 ttgtaatgaa cgaatctcag ccgaagaaaa agaagccaaa accggaggtt attgaagttg
     3421 aacaagagcc tcagtcaaag gctgaccagt ctctggctgg ggaagataaa caaggcgaga
     3481 aaactcggtt ttcaaattcc ttttctcaag cctacgcgga ggcaaatgcc gtgccgggat
     3541 ccgtgagccg ggtttcgata aaaaaggcgg acaaattttt tcgcggttcg aggaagctga
     3601 aaaaagattt agaaattgca agagtaggcg gcggtgcaaa gaaatttttt gttttttttg
     3661 ctggaatatt cgtgcttttg gccgcattgg ccgctaccta tttccttttt cccaaagcga
     3721 ccctcgtttt gcagatcaaa aatcaggaaa aatccgtttc acttgatctg accgccgccg
     3781 cgcagaacaa cgcgacaaat atcgctgata aaactttgcc ggcgaacctt gagcaaatca
     3841 cgaaagaggt gacggaggat ttcgacgcga ccggatcgaa ggatgggggt gcgaaagcga
     3901 caggccaagc ggttatatac aacgaattca gcggcgaaaa ccagccgctc gtcgccacga
     3961 ctcgtctcga aacggatgac ggaaaaattt tccggatcac aaaaaacgtg gtcgtaccgg
     4021 gaatgacgaa tgttggcgga gaaacaaagc ccggcgcgat cgaagtggac atagcggccg
     4081 accagccggg agaaaattat aacatcgatc ccgcaaactt caagattccc ggattcaagg
     4141 atactccggc aaaatatgaa aaattttacg cgaagtcgtc aaatcctaca acaggcgggt
     4201 catcaggcgc ggccaaagtg gtcacggcgc aggatattgc ctctgcgaaa gagaaaatag
     4261 ctgttgatgg aaaaaaggcg gccattgcgg a
//
LOCUS       DATKGS010000130         2531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_82586, whole genome shotgun sequence.
ACCESSION   DATKGS010000130 DATKGS010000000
VERSION     DATKGS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82586"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="murF"
                     /locus_tag="VMQ48_03280"
     CDS             <1..1181
                     /gene="murF"
                     /locus_tag="VMQ48_03280"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HUD09084.1"
                     /translation="IFSVLSSKFRVRKNEKNYNNEIGLPLTILGIESGGGSPWKWVVV
                     FFKAIGIVFFSSRKSYPEILVLEMGADRPGDIKYLVDFLQPEIGVMTTVGISHLEFFK
                     DKKNIAKEKGYLVRALNKENMAVLNWDDEEVRVMAESIKSKKISYGFSDGADIQASDI
                     FFGYERMKDLRGGDISKIKGISFKLSFEGTTLPVRLMRSVGRPQISAVLAAAAVGIHF
                     EMNLIEIAEALKDFQAPVGRLNLIDGIKNTVIIEDSYNSAPQSAIAALEVLEKIDARR
                     KIAALGDMLELGEAAEEGHREVGRYVAEVADILFAVGEKAKFIADEAEKMGLDKNKIF
                     CYTDSSEAKIPIQNMLQEGDVILIKGSQGARMERISKEIMRNPEDAEKLLPRQTAEWK
                     "
     gene            1766..1978
                     /locus_tag="VMQ48_03285"
     CDS             1766..1978
                     /locus_tag="VMQ48_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09085.1"
                     /translation="MVDLKEVCAMKDRRIFASELPASDGKGMNHQVRFDEVYRTPEEL
                     SSQDELLNFPWAFKYVFGKRVRPGRA"
     gene            2104..2175
                     /locus_tag="VMQ48_03290"
     tRNA            2104..2175
                     /locus_tag="VMQ48_03290"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2137..2139,aa:Glu,seq:ctc)
BASE COUNT          674 a          531 c          691 g          635 t
ORIGIN      
        1 cgattttttc cgtgctttct tcgaaattcc gggtgcgcaa aaacgaaaag aactataaca
       61 acgaaatcgg cttgccgctc acaattttgg gaattgaaag cggaggtgga agtccttgga
      121 aatgggtggt cgtatttttc aaggctattg gcatcgtttt cttttccagc cggaaaagct
      181 atcccgagat tctggtcctt gaaatgggcg cggaccggcc gggagatatc aaatatctgg
      241 tggattttct tcagccggaa atcggggtaa tgacgacggt tgggatcagc catctggaat
      301 ttttcaagga taaaaagaat atcgccaaag aaaaaggcta tcttgtacgg gcgctgaaca
      361 aagaaaatat ggcggttttg aattgggacg acgaggaagt ccgggtgatg gcggaaagta
      421 taaagtcaaa aaaaatatcc tacggctttt ccgacggggc ggatattcag gcctcggata
      481 tatttttcgg atatgaaagg atgaaggatc tgcgcggcgg agatatcagc aagatcaagg
      541 gcatcagttt caagctgagt ttcgaaggaa caactttgcc ggtgcgtctg atgcgctcgg
      601 tcggacggcc gcagataagc gccgtgctcg cggcggcggc ggtcgggatc catttcgaaa
      661 tgaacctgat tgaaattgcc gaagcgctca aagatttcca ggcgccggtg gggaggttga
      721 acttgatcga cgggatcaag aatacggtga tcatcgaaga cagctacaat tccgcgcccc
      781 agtcggcaat cgcggcgctg gaggttctgg aaaaaattga tgcccggcgc aaaatcgcgg
      841 cgcttgggga catgcttgag cttggagaag cggccgaaga aggccatcgt gaggttggga
      901 gatatgtggc cgaagtggcg gatattctgt tcgcggtcgg agaaaaagcg aaatttatcg
      961 ccgatgaagc ggaaaagatg gggcttgaca agaacaaaat tttctgctac actgattcgt
     1021 ctgaagctaa aatccctatt caaaatatgc ttcaggaagg agatgttatt ttgatcaaag
     1081 gttcccaggg cgcgcggatg gaacggattt ccaaggaaat catgagaaat cccgaagatg
     1141 cggaaaagct tctgcccagg caaacggcag aatggaaata ggccttccat aaagcaaatt
     1201 tggcctgtgg ataagtcaat ttgacgcaag ggcggaatta ggcatacaat aaaagtgcgt
     1261 caaacacaaa tacaattgaa ttgaggtacg tcggttgaat taatagggga taaccgttgt
     1321 acctttacac aacgagaaac gatctgtctt taatgcggtc gttttttgtt taaataacta
     1381 actgcggtta aataaatgag gaggtaaagt ccgatgtgta cgattgggtg tactgaatgc
     1441 cgagcccatt acgaaaacgg aaaaccggaa tccgttgata acagccacgg cctttgcccc
     1501 aagtgcctgc gactaagtct tctgggaact ttcagaaagc agcagcggaa agaaaatctt
     1561 cctgactgcg caggtcagtg cttcggcaat tgcgccacga cgtggtgcac ttactggcct
     1621 gtatgcgtca aggaatttcc gacttgggct gattttcagg aagtggaacg gagactggcg
     1681 gatcgcagga aagctctttg tgggaagtcg tgtggttatc aacaccacga agtgatggcg
     1741 atggcatcgg ctgctggtta atcctatggt cgacctcaag gaggtgtgtg ccatgaagga
     1801 tcgccgtatt ttcgcgagtg agcttcctgc ttccgatgga aaaggtatga atcatcaagt
     1861 gagatttgat gaagtctacc ggacgccgga agaactttcc tcacaagatg agttgctgaa
     1921 ttttccctgg gcgttcaaat atgtctttgg gaaaagagtg cgtcctggcc gcgcctaaaa
     1981 aggtgcgaga aaaggaggac aaagttttgc gaaaccgcag acgaagagcc ccgagtcaca
     2041 actgacccgg ggccttttct attggacaaa aagaaaaaat tcttgtattt tgtatattgt
     2101 tccggcccca tcgtctatcg gttaggacat taggttctca tcctaaaaag aggggtccga
     2161 ctccccttgg ggctacaaga aaaaaaagac cctcgacgat gttctgtcga ggggtggagt
     2221 gacttatgct gttagcttcg gttgtgttgt tgatgaatat tcaatgcgca tttgcgcggg
     2281 taggagatcc ccaggaacgt tcttcaatgc ttctatatat tgccgttctc gtgccctcgc
     2341 ttttagccag tcatctgtta tatgccgctg gttaacaacg gtcgctttgc cgcagccgct
     2401 gcatctaact gactcgcctt catgtttata gcgagcgtct cctagatcat tcccacatgt
     2461 tgctctcttt ctagtgctat gtttgcacct catccactgt ttctcccttt tttcaatgaa
     2521 tgaattcact c
//
LOCUS       DATKGS010000131         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_66055, whole genome shotgun sequence.
ACCESSION   DATKGS010000131 DATKGS010000000
VERSION     DATKGS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66055"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..259)
                     /locus_tag="VMQ48_03295"
     CDS             complement(38..259)
                     /locus_tag="VMQ48_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09086.1"
                     /translation="MLSIINILYLVIVLIFLAFGAAIVFHLLYYKINRHVSGMMSLIF
                     VIGAALLLISNFILFKQVAWEKIFSIFKL"
     gene            complement(264..1313)
                     /gene="cax"
                     /locus_tag="VMQ48_03300"
     CDS             complement(264..1313)
                     /gene="cax"
                     /locus_tag="VMQ48_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HUD09087.1"
                     /translation="MLNKIFAGMLIFLPVAVMAHFLNFSPILVFSLSALAIVPLAKFI
                     GEATEELSVYTGSALGGFLNASFGNATEFIIGIFALRAGLVEVVKASITGAIVGNLLL
                     VLGAAIFVGGLKFKKQKFNKTAAMTSGATLLLAVIALVIPAIFHQTAPEVSGKITQEL
                     SVFVSIFMLIIYLASLLFILFTHKHLYTEEVGKLEAKWSKMKSIIILLAATIAVAWMS
                     EILVGSIEPIVKSLGWTELFIGVIFVAIIGNAAEHTSAITMAVKNKMDLALQISIGSA
                     TQIAMFVAPVLVLVSLFFRTPMSLIFNIFELVAIVLSVLIANLITEDGESNWFEGLQL
                     LMAYAIMGVAFFFHP"
     gene            complement(1345..>2044)
                     /locus_tag="VMQ48_03305"
     CDS             complement(1345..>2044)
                     /locus_tag="VMQ48_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09088.1"
                     /translation="MFDEIRRGFSSSGGAQTDSKPPQNVVAFRHGTVKPAKQYSPGAS
                     LNYEFAKPGFIKAGNAGPSDSTAEKTSTPPLEATKKKMAKKYTPGSSLDYEFAKPGLQ
                     SEKESEKKKDVEIKPVAPEDYTKIPGTPQKATETAEETNAASPEATKKKPAGNYSPLD
                     DRLKYEYEKTDSPEAGSSGSSSRNNSPNTLAKMAPLMNIVRKKMLAEKEGDGSGKKRG
                     FSGAMAATMRNKQN"
BASE COUNT          548 a          456 c          472 g          568 t
ORIGIN      
        1 cgtgttgtcc cggaaaattt atttctccaa acataaatta taatttaaaa atggaaaata
       61 ttttttccca tgccacctgc ttgaacagta taaaatttga aatcaaaaga agtgcggcgc
      121 cgatgacgaa aatgagcgac atcatccctg aaacatggcg gttgatcttg taatagagca
      181 gatgaaaaac aattgccgct ccaaaagcga ggaaaatgag gacgatgacg aggtaaagaa
      241 tgttgatgat agagagcata tgtttatgga tgaaagaaaa aagctactcc cattatagca
      301 taagccatca gaagttgaag cccttcaaac cagttgcttt ccccgtcttc ggtgatgaga
      361 ttggcgatga ggaccgacaa aacgatggcg acaagttcaa aaatattgaa gatcaggctc
      421 atcggcgtac ggaagaacaa actgaccaga accaaaaccg gggcaacaaa catggcgatc
      481 tgagtggccg acccaatcga aatctgaagg gcaagatcca ttttgttttt gaccgccata
      541 gtgatggcgg aagtatgttc ggccgcgttg ccgatgatgg cgacgaaaat cacaccgatg
      601 aaaagttccg tccagccaag actcttcacg atcggttcga ttgacccgac caggatttca
      661 ctcatccaag cgacggcaat agtcgcggcg aggaggataa ttatactttt cattttgctc
      721 cattttgctt caagctttcc aacttcttcg gtatagagat gtttgtgtgt gaaaagaatg
      781 aaaagaaggc ttgctaaata tatgatcaac atgaaaattg aaacgaaaac gctcagctct
      841 tgagtaattt ttccactgac ttccggcgcc gtctgatgaa aaatcgccgg tatcacgaga
      901 gcgatcacgg ccaaaagcaa agtcgcgccg ctggtcatgg cggccgtttt gttgaatttt
      961 tgctttttga atttcaatcc gccgacaaaa attgccgcgc ccaaaaccag cagaagattg
     1021 ccaacgatcg cgccggtgat ggaggccttc acgacttcaa cgagtcccgc ccgcaaagca
     1081 aaaatgccga tgataaactc ggtcgcgttt ccaaaagagg cattcaaaaa tccgccgagc
     1141 gccgagccgg tataaactga aagctcttcc gtcgcttcgc cgataaactt ggcgagtggc
     1201 acaattgcga gtgccgacaa agaaaaaaca agaatcggcg agaaattcaa aaaatgagcc
     1261 atgacggcga ccggaaggaa aataagcatt ccggcgaaaa ttttgttgag catatttgtt
     1321 tttgctagtt tttatttttg cgagttagtt ttgcttgttt ctcattgtgg ctgccatggc
     1381 gccggaaaaa cctctttttt tccccgatcc atcaccttcc ttttcagcta gcattttttt
     1441 tctcacaata ttcatcaagg gcgccatttt tgccagtgta ttgggagaat tattcctcga
     1501 ggatgaaccg gagcttcccg cttcaggaga atccgttttt tcatattcat atttcagcct
     1561 gtcgtcgaga ggggaatagt ttcccgctgg ttttttcttt gtcgcttccg gcgaagcggc
     1621 gtttgtttct tcggcggtct ctgttgcttt ctggggtgta ccgggtattt tggtgtaatc
     1681 ttctggtgcg acgggcttga tttcaacatc ttttttcttc tccgactcct tttctgactg
     1741 gaggcccggt ttagcaaatt catagtctaa gctagaccct ggagtatatt tttttgccat
     1801 ctttttcttt gtcgcctcta gaggaggagt gctcgtcttt tcagctgttg aatcagatgg
     1861 accggcgtta cccgccttaa taaatcccgg ctttgcgaat tcataattca agctggctcc
     1921 gggagaatat tgttttgccg gttttaccgt tccatgtctg aaggcaacca cattttgcgg
     1981 cggtttggaa tctgtctgtg cgcctccgga agaagagaat cctcttctta tttcatcaaa
     2041 catt
//
LOCUS       DATKGS010000132         4896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_37394, whole genome shotgun sequence.
ACCESSION   DATKGS010000132 DATKGS010000000
VERSION     DATKGS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37394"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /gene="dnaE"
                     /locus_tag="VMQ48_03310"
     CDS             complement(<1..2138)
                     /gene="dnaE"
                     /locus_tag="VMQ48_03310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HUD09089.1"
                     /translation="MPENKPKIPFTHLHVHSHYSLLDGLAKIDELLDRAKELGMESLA
                     LTDHGVLYGAIEFYQKAKERGIKPIIGCEMYVAPNGLANKRAKIDDTRHHLILLAKNE
                     KGYKNLLQLVSIAHLEGFYYKPRIDKELLKKYSEGLIGLSACVEGEVPSLVLSGKIEE
                     AEKSAREYEAILGKGNFFLELQHHPKFANQKIANDGVIEISRRTGIPLVATTDMHYVH
                     LADAPIQDILLCVQTNRKVSEQNRMNLMDFELYMRSPEKMLEDFADVPEAIENTMRVA
                     EMCNLEIELGNMKLPFYEVPDGFTPETYLRKLCEEGLAERYGKNITQVHRDRMEFELS
                     VVEKTGYASYFLIVQDFVNWAKDQGIVVGPGRGSAAGSFVSYITGITNIDPIKYNLLF
                     ERFLNPERISMPDIDLDFADARRDEVLAYVRQKYGKDHVAQIITFGTMAARAAIRDAG
                     RALGYPYGFCDQTAKFIPTGMSIRDCIAGVPEVSNLYKTDPAGKKLLDSAARLEGVCR
                     HASMHACGVVITPEPVVNYSPLQYLSGDTEHMVTQYSSSTKSSYIEKIGLLKMDFLGL
                     KNLTIIQHALKIIEKTKGAKINIDDIPLDDEKAYELLQKALTTGVFQLESGGMKRYLK
                     QLEPTTFEDIIAMVALYRPGPMDWIPDFIARKHGRKKIVYIHPKLEPILENTYGVAVY
                     QEQIMQISQALAGFSLSEADILRKAVGKKIP"
     gene            complement(2260..2886)
                     /locus_tag="VMQ48_03315"
     CDS             complement(2260..2886)
                     /locus_tag="VMQ48_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09090.1"
                     /translation="MQNKKLLSAGLIGCLVFAISAMTALPTLAATKNKTVENMVVKSS
                     DDSKETIKAVQAGYTYTIDASSATLKKESKSGKSMNFSDFKDGDVISVKGSFNKRDVT
                     ATSIWDLSITKSATMYGVVDSINTATQTVKIKTIKRGKLTVAISKSTKVTYGGKKKKF
                     TDIRQDDKILVTGAWDSSKDTITKTKNFDILVKDDYKKLDLDSTDASI"
     gene            3111..3833
                     /locus_tag="VMQ48_03320"
     CDS             3111..3833
                     /locus_tag="VMQ48_03320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HUD09091.1"
                     /translation="MIHPHLEKEKFHWEQGRKFVAGIDEAGRGPLAGPVVAGAVIVRP
                     DIIDEIKNTPEFKIIRDSKTLSEKQREKAYDFITRHFQWGVGISDEKTIDRINILQAA
                     FLAMKKAISDLKSKIKCEPEIILVDGRSPVPNISIFQESIVGGDKFVFSISAASIIAK
                     VTRDRMMLDFHEKYPVYGFAKHKGYGTKMHFEMIGKYGLCEIHRKSFKFPGNPNIKAQ
                     MSNQAQNQKYKYPLSFSHSDLI"
     gene            3937..4143
                     /gene="rpmF"
                     /locus_tag="VMQ48_03325"
     CDS             3937..4143
                     /gene="rpmF"
                     /locus_tag="VMQ48_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HUD09092.1"
                     /translation="MSVPKQHHTKGRRDRRRVRFIIDPKKLIACSHCKKMTKQHEACP
                     YCGFYKGKEVVDMSKKTAKKKPKK"
     gene            4240..4656
                     /gene="speD"
                     /locus_tag="VMQ48_03330"
     CDS             4240..4656
                     /gene="speD"
                     /locus_tag="VMQ48_03330"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HUD09093.1"
                     /translation="MKNETMGKDTCSFGVHFTLDGYGGDPEKLNDPELMERILNELPD
                     KLGMDKLTEPIVKYAEPRHLKDSGGYSGFVIIAESHISIHTFPKKKFVSIDAYTCKDE
                     MDTERIEKYFRDAYDLQETEVQFFKRGLKFPKEDLA"
BASE COUNT         1278 a         1102 c         1190 g         1326 t
ORIGIN      
        1 ggaatttttt tgccgaccgc ttttcgcaaa atatccgctt cggaaaggga aaaaccggcg
       61 agtgcttgcg aaatttgcat aatctgttct tgatataccg ccacgccgta ggtattctcg
      121 agaataggct ccagttttgg gtgaatatag acaatttttt tgcggccgtg ttttctggcg
      181 atgaagtcgg ggatccaatc catcggtccg ggtctataga gagccaccat agcaatgata
      241 tcctcgaaag ttgtaggctc aagctgtttc agatatcttt tcatcccccc cgattccaat
      301 tggaaaacgc cggtcgtcag agctttctgc aaaagctcgt aggctttttc gtcatcgagg
      361 ggaatgtcgt caatattgat ttttgcgcct tttgtttttt cgatgatttt cagcgcgtgc
      421 tggatgatgg tgaggttttt gagtcccaaa aagtccattt tgagaaggcc gattttttcg
      481 atgtagcttg atttggttga agaagaatac tgggtcacca tatgttccgt gtctccggag
      541 agatattgaa gaggagaata gttcacaacc ggctccgggg tgatgacgac accgcaagca
      601 tgcatcgaag catgccggca gacgccctcg agccgcgccg cgctgtcgag cagttttttt
      661 ccggccgggt ccgttttgta taggttggaa acttcgggga cgccggcaat gcagtcgcgg
      721 atcgacatgc ccgtcgggat gaacttggcc gtctggtcac aaaacccata tggatagcca
      781 agcgcgcgtc cggcgtcgcg gatggcggct ctcgcagcca ttgttccgaa agtgatgatt
      841 tgggcgacgt ggtctttgcc gtatttttgc cttacataag ccaaaacttc atcacggcgg
      901 gcatcggcga aatcgaggtc gatgtccggc atggagatgc gctccgggtt caaaaaccgc
      961 tcaaaaagca aattgtattt gatcggatcg atgttggtga tgccggtgat atacgaaacg
     1021 aaacttccgg cggcgctgcc gcgtcccggc ccgacgacga ttccctggtc tttcgcccaa
     1081 ttcacaaaat cctgaacgat caggaaatat gatgcatagc cggttttttc cacgaccgag
     1141 agttcgaatt ccatgcgatc gcgatgaact tgcgttatat tttttccgta tctttcggcg
     1201 agtccttctt cgcacaattt gcgaagatat gtttcgggcg tgaatccgtc cggaacttca
     1261 tagaagggga gtttcatatt tcccagttca atctcgaggt tgcacatttc ggcgacgcgc
     1321 atcgtgtttt cgatcgcttc cgggacatcc gcgaaatctt cgagcatttt ttcgggactg
     1381 cgcatataca attcgaaatc catcaggttc atccggtttt gctcgctcac tttgcggttc
     1441 gtctggacac aaaggagaat atcctggatc ggcgcgtccg ccagatgaac atagtgcata
     1501 tcggtcgtgg caaccagggg aatgcccgtt cgccgcgaaa tttcaatcac gccatcattg
     1561 gcgatcttct ggtttgcaaa tttcggatga tgctggagtt caagaaaaaa attccctttt
     1621 ccgagaatgg cttcgtattc gcgcgccgat ttttcggctt cttcgatttt tccggagagg
     1681 acgagtgagg ggacttcgcc ttcgacgcag gcagagaggc cgatgaggcc ttcggaatat
     1741 tttttcaaaa gttctttgtc gatgcggggc ttataataga atccttcgag atgggcgatg
     1801 gaaaccaatt gcaaaagatt tttgtagcct ttttcgtttt tggccagcag aatcaaatga
     1861 tgccgcgtgt cgtcgatctt ggcccgtttg ttggcgaggc cgttcggagc aacatacatt
     1921 tcgcagccga tgatcggttt gatgccgcgt tccttggctt tttgatagaa ctcaatcgcg
     1981 ccatagagca caccgtggtc ggtgagggcg agggattcca ttcccagctc cttggcgcgg
     2041 tcaagaagct catcgatctt ggcgagaccg tcgagtaggg aatagtgcga atgcacgtga
     2101 agatgagtga atggaatttt aggcttattt tctggcataa taaaaaatga acctccccta
     2161 taaaaaggct ttgttgtttt tttcttatta tagctgattt tgcgaaaaca aaaaagggcc
     2221 tcgcagaact cgcgggccct tttccaattt caaaataggc tagatgctgg catctgtcga
     2281 atcaagatcc agctttttat agtcatcctt caccaaaata tcaaaatttt tcgtcttggt
     2341 gattgtatct ttggaagaat cccaggcgcc ggtgacgagg atcttgtcgt cttgccgaat
     2401 atcagtgaat ttctttttct ttccaccata agtcactttg gtcgatttcg aaatggcgac
     2461 tgtcagctta ccccgcttga ttgtcttgat cttgacagtc tgggtggccg tgttgatcga
     2521 atcaaccacc ccgtacatgg tagccgactt ggtgattgac aagtcccaga ttgaggtggc
     2581 ggtcacatcg cgcttgttga acgaaccctt tacactgata acatctccgt ccttgaaatc
     2641 tgaaaaattc atgctctttc cgcttttact ctccttcttt agagtagcgc tcgaagcgtc
     2701 aattgtatat gtgtagccgg cctgtacggc tttgatggtt tctttgctgt catcagaact
     2761 cttcacgacc atattctcca cggttttgtt ttttgtcgcg gccagggttg gcaaagccgt
     2821 catcgccgaa atcgcaaaaa ccaaacagcc gatcagaccc gcagacaata attttttgtt
     2881 ttgcactttg tttttattta ggattagtta gctatattat acagcaattt cgggtgtaga
     2941 taaatacttt gtcaatactc gcatcctccc ctcctgccaa ggaggggata agaggggtgg
     3001 ttttctctgc acgactgaaa cccctccgcc cttcgggtac ctccccttgg caggggagga
     3061 ggtatagaaa aatactttat cctttatggc caatgcctta tactaaacct atgatccatc
     3121 ctcatctcga aaaggaaaaa ttccactggg aacaagggcg gaaatttgtg gccggaattg
     3181 atgaagcggg gaggggacca ctggccggac cggtggtggc gggagcggta atcgtgcggc
     3241 cggatatcat cgatgaaata aaaaatacgc ccgaattcaa aatcatccgt gattccaaaa
     3301 ctctctcgga aaagcaacgt gaaaaagcct atgatttcat cacgcgccat ttccagtggg
     3361 gggttggcat ttctgacgaa aaaacgattg atcggatcaa tatcctccaa gcggcttttt
     3421 tggccatgaa aaaggcaatt tctgacctca aaagcaagat caagtgcgaa ccggagatta
     3481 ttctggtcga cgggcgcagt ccggttccaa acatttcaat tttccaggaa agcattgttg
     3541 gcggggacaa gtttgttttt tccatctccg ccgcgtccat catcgcgaaa gtgacccgcg
     3601 accggatgat gctcgatttt cacgaaaaat atcctgtcta cggatttgca aaacacaaag
     3661 gatacgggac gaaaatgcat tttgaaatga tcggaaaata cgggctttgc gaaattcacc
     3721 gaaaaagttt taagttccct ggaaatccaa atatcaaagc tcaaatgtca aatcaagctc
     3781 aaaatcaaaa atacaaatat cctttgtcat ttagtcattc ggatttgatt tgaaatttgg
     3841 attttggatt ttgaaattta ttcagttggc tattgacaaa tactcacaaa aatatagaat
     3901 tgagtgtaga ctaatttttt atctgtactg aaatttatgt ccgtaccaaa acagcatcac
     3961 acaaaaggaa gaagagatcg ccggagagtc cgcttcatca ttgatccgaa aaagttgatc
     4021 gcttgttctc attgcaaaaa aatgacgaag cagcatgaag cttgtccgta ctgcggtttt
     4081 tataaaggaa aagaagttgt tgacatgagc aaaaaaacgg caaaaaagaa gccgaagaaa
     4141 taagcaaaat aaccaataaa tttaataaac ctaatcctgt tgaattgtgt tcaaagcgca
     4201 actccgatgg aacctcggag gattcaacag aagcaaaaaa tgaaaaacga gactatgggg
     4261 aaggatacct gcagcttcgg agtgcatttc acgctcgacg gctacggggg cgatcctgaa
     4321 aagctcaatg acccagagct aatggaacgg attctcaatg agcttccgga taaactggga
     4381 atggacaaac tgacggaacc gatcgtgaaa tacgccgagc cgcggcattt gaaagacagc
     4441 ggcggctatt ccgggttcgt gatcatcgcc gaatcccaca tcagtatcca cactttcccc
     4501 aagaaaaagt tcgtgagcat tgacgcctat acctgcaaag acgagatgga cacggaaaga
     4561 atcgaaaaat atttccgcga tgcgtacgac ctccaagaga cggaagtcca atttttcaaa
     4621 cgcggattga agtttccaaa agaagatctg gcctaggagg cagtcccaac aaccaagacc
     4681 cgggcggagc gatcccctgg gtctttggct ttacttgggg gcaaaaaaag attattattc
     4741 aagcaatggc aaagaaacac gaaaattatt gggaattggt ctgggcgctc gctaggactg
     4801 atttcaaatt gcgctaccat gggtcagtcc tcggctatgt ctgggcgctt ttgaagccac
     4861 ttctcacgtt cttgattttg aatttcgttt tttcga
//
LOCUS       DATKGS010000133         4902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_37739, whole genome shotgun sequence.
ACCESSION   DATKGS010000133 DATKGS010000000
VERSION     DATKGS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37739"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..680
                     /locus_tag="VMQ48_03335"
     CDS             162..680
                     /locus_tag="VMQ48_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09094.1"
                     /translation="MDINIFAIFQSLFSGFAHSSFFAFIKILAGFIIAILLIADILLI
                     SKRIRGDWKIAFHGAKVPNLKKTKYMERWEGIKNNAESGDMAKAKIAMIEADQMFGET
                     LEKIGYKGKDTGERISAVKPGQLIGLEEAKFSRVVFKKIVHSSSHEMNIVDIQAALGG
                     YEKVFRGLELLD"
     gene            850..1857
                     /gene="gap"
                     /locus_tag="VMQ48_03340"
     CDS             850..1857
                     /gene="gap"
                     /locus_tag="VMQ48_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HUD09095.1"
                     /translation="MKIKIAINGFGRIGRPALKIALEKENIEVVAINDLADDKTLAHL
                     LKHDSSYGTYARDVKVGSGEIIVDGKSIKSFAEPDPTKLPWKDLGVDVVLECSGVFND
                     RSSAEKHLQAGAKKVILSAPPKSDDIPVYLIGVNESEMKSEDDIISNGSCTTNCLAPM
                     IKVLDEKFGVEKGFLTTVHSYTNSQRILDLPHKDLREARSAGQNIIPTTTGAAKMVAK
                     AIKSMEGNLDGISMRVPTPVVSVTDFVGILKKEVSVEEINSAYREAAASNLKNILNIS
                     EEELVSMDFKGNPASAIVDLPLTMANGNLVKVVGWYDNEWGYANRLVEMAELAGNILK
                     I"
     gene            1865..2629
                     /gene="murI"
                     /locus_tag="VMQ48_03345"
     CDS             1865..2629
                     /gene="murI"
                     /locus_tag="VMQ48_03345"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HUD09096.1"
                     /translation="MIGVFDSGVGGLTVVKEIKKLLPDAPIVYFGDTARLPYGNKSAE
                     TIQKYSSEIVDFLQSKGCRTIVIACNTASALAADWLREKYPELEIFDVVSSGAEAVAE
                     TTKSKKVGVIGTTATINSGIYKKKISQLNPKIEVFMKACPLFVHLVEENWIKRPETKK
                     IARAYLKELKLQKIDTLLLGCTHYPLLEKVVSGVMGKRTKVIASGEKLAKELKSKIKD
                     HKKSEDEYFVSDLTPHFEKLAKLILNKKIKIKKCTI"
     gene            2617..4191
                     /locus_tag="VMQ48_03350"
     CDS             2617..4191
                     /locus_tag="VMQ48_03350"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HUD09097.1"
                     /translation="MYDIVIRNGTVVDGTGKEKYLADVAVKDGKIAKIGKMKWTRGKK
                     EIDAAGKVVSPGFIDMHNHSDSYWRLFLEPKLPSLVYQGVTTIVGGNCGASIAPLSSG
                     KIIESVQKWADIKEVNVNWLSMREFREEMEKRKMGLNFATLVGHGTLRRGIIGDDMRR
                     LADNELELFKNILEKSLEAGALGFSAGLAYSHEREAKWDELEAMLEVLKRHNKIFSLH
                     LRDEGKDILSSLDEAIDLAGQTGVSLQISHFKVMGEKNWPLAEQALQKIEEAQKAGID
                     VNFDVYPYTETGSVLYVYLPAWAAEGGKRMLLRRLKEKHLREKIVSEMKDIHEYPYEK
                     AIVAISSLDKNLPERRISKIAEARGISAEEVVVDLLVASEGRVITLLECLDEENVRKA
                     IAHPLSIIASDGSGYDINHKDTGEIVHPRCFGTFPRVLGKYVREEKLLTLEEAVKKMT
                     SMPAKRLGFVDRGILQNNFEADIVVFDPEKIIDKSTIEDPYQYAEGVSEVVVNGKLVL
                     EEGKMTGEMAGRFLAS"
     gene            4194..>4902
                     /locus_tag="VMQ48_03355"
     CDS             4194..>4902
                     /locus_tag="VMQ48_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HUD09098.1"
                     /translation="MKKSDFKDKKVTVMGVGLHGGGVATVRFFAECGARVIATDLKTE
                     KELAQSIEKLKDLKNVKFVLGQHRIEDFENVDMVVKSPAAPWTSKPIQAALKKKIPVE
                     MDSSIFFKLCKLPIIGVTGTKGKTTTSTLIAEILKTSGKKVFTAGIGQKPILNLLDEI
                     GDIKDGVVVFELSSWRLSALGREKLSPHIAVITNVYPDHLNYYGTMEKYIVDKKYIFA
                     NQKPEDYVVLNYDNEASR"
BASE COUNT         1560 a          878 c         1255 g         1209 t
ORIGIN      
        1 ggttggattg caactcatgg ggaaatggtt tgacgaggaa acgctgctta atacggcgta
       61 tgtttacgaa caatcttagt ttctaaacaa attcattcag acatttataa atttagtcat
      121 tgtttgaaaa ttgaaaatta gaaattgaaa attttagata tatggacatc aatatttttg
      181 ccatttttca atctctcttc agcggttttg cgcattcgtc ttttttcgca ttcataaaga
      241 ttctggccgg attcatcatt gccattcttc tgattgccga tattctgctc atatccaagc
      301 ggatccgagg ggattggaaa atcgctttcc atggagcaaa agtgcccaat ctcaaaaaaa
      361 caaaatatat ggaaagatgg gaaggcatca aaaacaacgc ggaaagcgga gatatggcaa
      421 aggcaaaaat cgcgatgatc gaagccgatc agatgtttgg agagacgctt gaaaaaatcg
      481 gctacaaagg caaggacacg ggagaaagaa tctcggcggt gaaaccgggc cagttgatcg
      541 gactcgagga agccaaattt tcgcgtgttg ttttcaaaaa gattgttcac tcttcaagcc
      601 acgaaatgaa cattgtggat attcaagcgg cgcttggagg atatgaaaag gtgtttcggg
      661 ggctggaact attagattaa ttttcaatgt caaaattcca aaattttcaa acaattctca
      721 aatgaccgaa tttcagaaaa attttgaaat tgaataattc agtcattgtt tgaaaattga
      781 aaattctgaa attataaaat ttaaacgaat tatttgtaat attgttaata aaaaataaaa
      841 acatatatta tgaagataaa aattgctatc aacggttttg gccgaattgg ccggccggcg
      901 ctcaaaattg cgttggaaaa agaaaatatc gaagttgtgg cgatcaatga tctcgccgac
      961 gacaaaactt tggcgcatct cttgaagcac gacagttcgt acggaactta tgcgcgagac
     1021 gtgaaagttg ggagcgggga aataatcgtg gacggaaaat cgatcaaaag ttttgcggag
     1081 ccggatccga caaaacttcc atggaaagat ctgggggtgg acgtcgtcct cgaatgcagc
     1141 ggagttttca atgatcgctc gagtgccgaa aaacatctcc aggccggggc gaaaaaggtt
     1201 attttaagcg cgccgccaaa aagtgatgat attccggtgt atctcatcgg agtgaatgag
     1261 agcgaaatga aatccgaaga tgatattatt tccaatggtt cctgcacgac caattgtctt
     1321 gcgcctatga tcaaagtgct cgacgaaaaa ttcggcgtgg aaaaaggttt tttgacgact
     1381 gttcactcat atacgaacag ccagcgcatt ttggatcttc cccacaaaga tttgcgtgag
     1441 gcgcgatcag ccggacaaaa tatcatccca acgacaacgg gcgcggccaa aatggtcgca
     1501 aaagctatca agagtatgga aggaaatttg gacgggatct caatgcgcgt gccaacgccg
     1561 gttgtttccg tgacggattt cgtgggaata ttgaaaaaag aagtaagcgt ggaagagata
     1621 aattccgcct atcgcgaagc cgccgcaagc aatctaaaaa atattttaaa tatctccgaa
     1681 gaagaactgg tttcaatgga tttcaaaggc aatccggcga gcgcgattgt cgatctgccc
     1741 ctcacgatgg cgaatggaaa tcttgtgaaa gttgtgggtt ggtatgacaa tgagtgggga
     1801 tacgcgaacc ggttggtgga gatggcagag ttagcgggaa atattttaaa aatttgaatt
     1861 aattatgata ggagtttttg acagcggagt ggggggattg acagtcgtca aagagatcaa
     1921 aaaattactg cccgatgcgc cgatcgtata ttttggcgat acggcgagat tgccgtatgg
     1981 aaacaaaagc gctgaaacaa ttcagaaata ttcttcggaa attgttgatt ttttgcaaag
     2041 caaaggatgc cgaacaatcg tcattgcttg caatacagct tctgctctcg cggccgactg
     2101 gcttcgcgaa aaatatccgg agcttgaaat atttgatgtg gtttcatctg gagcggaagc
     2161 ggtggccgaa acaactaaga gcaaaaaagt cggagtgatt gggacgacgg cgacaataaa
     2221 cagcggtata tataagaaaa aaatttccca gctcaatccg aaaatcgaag tattcatgaa
     2281 agcctgtccg ctttttgtcc atttggttga agaaaattgg atcaagcgtc ccgaaacgaa
     2341 aaaaatcgcg agagcttatc tcaaagaatt gaaattgcaa aaaatagata cattgctttt
     2401 gggatgcacg cattatccat tgcttgaaaa agttgtttcc ggcgtgatgg gaaaaagaac
     2461 gaaagtgatt gcctcgggag agaagctggc aaaagagctg aaaagcaaga tcaaagatca
     2521 caaaaaaagt gaggatgaat attttgtcag cgatcttacg ccgcatttcg aaaaactagc
     2581 caaattaata ttaaataaaa aaattaaaat aaaaaaatgt acgatatagt cattcgaaac
     2641 ggtactgttg tcgacggaac gggaaaagaa aaatatttgg ctgatgtggc ggtcaaggac
     2701 ggaaaaattg ccaaaatcgg aaaaatgaaa tggacgagag gaaagaagga gattgacgca
     2761 gccgggaaag tcgtttctcc cgggttcatt gatatgcaca accattcaga tagttattgg
     2821 cgattgtttc tcgagccgaa gctgccgagt cttgtctatc agggagtcac gacgattgtc
     2881 ggagggaatt gcggggcgtc aatcgcgcct ctttcgagtg gaaagatcat tgaatcagtt
     2941 cagaaatggg cggatatcaa agaggtcaac gtgaactggc tttcaatgag ggagtttcgc
     3001 gaggagatgg aaaaaagaaa gatgggactc aattttgcga cactggtcgg tcatgggact
     3061 ttgaggcggg gaattatcgg ggacgatatg cgccgtctgg ccgacaatga attggaactt
     3121 ttcaaaaata ttttggaaaa atcactggaa gcgggcgccc tgggtttttc ggcgggtctg
     3181 gcctatagcc acgagcgcga agcaaaatgg gacgaactcg aagccatgct tgaagttttg
     3241 aaaagacaca acaagatttt ttctttgcat ttgagagacg aaggaaaaga catcctgtcc
     3301 tcgctcgacg aagcgatcga tctggctggg caaacgggcg tttccttgca aatatcccac
     3361 tttaaagtga tgggtgagaa aaattggccg cttgccgaac aggcgcttca gaaaattgaa
     3421 gaagcgcaaa aagcgggaat cgacgtgaac tttgatgtct atccctacac ggaaacggga
     3481 tctgtactct atgtctatct tccagcttgg gcagcggagg gcggaaaaag gatgcttttg
     3541 cgccgactca aagaaaagca tttgcgggaa aaaattgttt ccgaaatgaa agatatccac
     3601 gaatatccct atgaaaaagc gattgtggcg atttcgtcgc tcgacaaaaa tttgcccgaa
     3661 aggcgtattt ccaagatcgc cgaagcgcgg ggaatttcag ccgaagaggt ggttgtcgat
     3721 cttctggtgg cgagcgaggg aagagtgatc acacttcttg aatgcttgga cgaagaaaac
     3781 gtgagaaaag caattgctca tccgctatca atcattgcat cggacggatc cggatacgat
     3841 atcaatcaca aggatacggg tgagatcgtt catccgcgct gctttgggac atttccgcgg
     3901 gtgcttggaa aatatgtccg agaagaaaaa ttgctgacgc ttgaagaagc ggtcaaaaag
     3961 atgacgtcga tgccggcaaa aagactgggc ttcgttgatc ggggaatctt gcaaaataat
     4021 ttcgaagctg atattgtggt ttttgatccc gaaaaaataa tcgacaagtc aaccattgaa
     4081 gacccgtatc aatatgccga aggcgtgagc gaagtggttg tgaacgggaa attggttttg
     4141 gaagaaggga aaatgaccgg ggaaatggca ggccgatttt tagccagcta aatatgaaaa
     4201 aaagcgactt caaagacaaa aaagtgacag ttatgggtgt tggcctccat ggcggcggtg
     4261 tggcaaccgt tcgttttttt gcggaatgtg gagcaagagt gatcgcgacg gatctcaaga
     4321 ctgagaaaga gcttgcgcag tcaatcgaaa aattgaaaga tttgaaaaac gtaaaatttg
     4381 ttctgggtca gcatcgcatt gaagattttg aaaatgttga tatggttgtg aaaagcccgg
     4441 ctgctccctg gacatcaaag cctattcagg ccgcgctcaa aaagaaaatt ccagtcgaga
     4501 tggattcgag tatttttttc aaactttgca agttgccgat catcggggtg acggggacga
     4561 aagggaagac gacaacatcg actttgatcg cggaaatttt gaagacgtcc ggcaaaaaag
     4621 ttttcacggc cgggatcggg caaaaaccga ttttgaattt attggacgaa attggcgata
     4681 taaaagacgg cgtggttgtt tttgaactat caagctggag attgtcggcc ttgggacgag
     4741 aaaaattgag tccgcatatc gcggtgatca caaatgtgta tccggatcac ctcaattatt
     4801 atggcacaat ggaaaaatat attgtcgaca aaaaatatat ttttgcgaat caaaagccgg
     4861 aagactatgt ggtcttaaat tatgataatg aagcgtcacg gt
//
LOCUS       DATKGS010000134         4420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_23219, whole genome shotgun sequence.
ACCESSION   DATKGS010000134 DATKGS010000000
VERSION     DATKGS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23219"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VMQ48_03360"
     CDS             <1..414
                     /locus_tag="VMQ48_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09099.1"
                     /translation="LITKKMEGNSNQQPKEITLTDLAAQMASGFERIDKEFKESRKHT
                     EKLIGKAIEELATSTQKEFLSLKTDIEGVKTDIEGVKSDIEGVKTDIEGVKSDTGEIK
                     ANLNKKVDVVIHNELEYRVEKTEEKLGLKPKLKFA"
     gene            complement(518..1318)
                     /locus_tag="VMQ48_03365"
     CDS             complement(518..1318)
                     /locus_tag="VMQ48_03365"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HUD09100.1"
                     /translation="MLDKKFLEEYIKKNGIPQKPRQNLTEYLQAEILAALYGSDYGQF
                     LSFIGGTCLRFAYNIERFSEDLDFDLIKNGFDCEGLAKYLEKKLKELGFKADARAKRT
                     KNIFIIYVKFSSVMKEMGLSPMDGQKLMIRFEADPNPPKHIKFESKRVNAYGKAFNIV
                     ANELDSVFAQKVLAIMKRPYQKGRDFYDLVWLLGQKNLEPNYAILQEKKIAVVNRAEL
                     VEMLQKKVAKSDLKQAVKDVEKFLFHPEQAKWILDLPKYLDDYAKAVL"
     gene            complement(1308..1901)
                     /locus_tag="VMQ48_03370"
     CDS             complement(1308..1901)
                     /locus_tag="VMQ48_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09101.1"
                     /translation="MMDYPVFTSSEVKNIFFGEKNLLSQIAFWEKKGHIRKLRKGVYV
                     LVNSAREIDPMAFASKIYDPSYVSLEYALYHHGIIPDVAWAYTSVTTRKPVKFSNDYG
                     DYYYQKIKPELFIGYRPLVEKNVSFNLAVPEKAIMDYIYFNKNKLIPKVDFWRELRIY
                     EEFKFNKKRIEEYKKMFDDKKVSILIDSLLEYQKNAR"
     gene            complement(2516..3919)
                     /locus_tag="VMQ48_03375"
     CDS             complement(2516..3919)
                     /locus_tag="VMQ48_03375"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUD09102.1"
                     /translation="MIKIQDLALKNAWWSDNQYKIEETDWEKREIYDDIVANMEHSLI
                     LNIVGLRRVGKSTILKQIIGRLLLKKKNPKNIFYFLFDYSNQIKKAEFLDEVLSAYFQ
                     DILNNPNLSLDEQVYVFLDEIQYIEDWQSVLKKFYDLSGKKIKFVVTGSQSVLLKGKY
                     SESLAGRIFDFYLPPLSFREFLTINHEAVKIFKKFDLFELPKRFGELGVYDSNFGRDI
                     ARLSREYITVGQFPETRKISDIEKKHEYITESVVGKVLDDCVRIFKVEKTDEFKLIAY
                     QLINNAGSIFELKNIGREVGLSFLTLEKYIEYLKEGYIAEVLYRHHKSLIKRGRMLKK
                     IYTPCVNFVCALNHFNADHVSEVPQAFGKIIENAVYNILHQKYKSNKIDNILSFWRQG
                     EKEIDFLVNKKDDQLPIEVKFSNKITSKDAMFLADYVKKHELNFGIVVTKNEINRKTI
                     SGKTIYYVPYYLILLMI"
     gene            4062..>4420
                     /locus_tag="VMQ48_03380"
     CDS             4062..>4420
                     /locus_tag="VMQ48_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD09103.1"
                     /translation="MPEFRKISIVVPIYNEEKTLGKIIAAVENADVFDLEKEIILVND
                     CSKDKSKEILEKYAGKHKVLHHKVNQGKGAALKTGFASATGDIVLIQDADLEYDPNEY
                     VNLLKPILDGKADVVFGS"
BASE COUNT         1347 a          891 c          817 g         1365 t
ORIGIN      
        1 ttgataacaa aaaaaatgga gggaaatagc aatcaacagc caaaagaaat aactctgact
       61 gatctggccg cgcagatggc aagcgggttt gagcggattg acaaagaatt caaagaatca
      121 cgcaagcaca ctgagaaatt aataggcaaa gcgatagaag aactggccac gtcaacccaa
      181 aaagaattct tgagcctcaa gacagatatc gagggcgtga agacggatat cgagggagtg
      241 aaatcagata tcgagggcgt gaagacggat atcgagggag tgaaatcaga cacaggagag
      301 atcaaagcca atctgaacaa aaaagttgat gtggttatcc acaacgaact cgaatatcgc
      361 gtggaaaaaa ccgaagagaa gctcggtttg aaaccgaaac taaaattcgc ctgaaaaaaa
      421 ccgcgaaaaa ttccggccgc gcgccggttt tttataccga agtagaaaaa tcttgaaaca
      481 ttattggagt tgggtgtaaa acgtaagcaa atttatatca gagaaccgct ttcgcgtaat
      541 catcaagata tttcggcaga tccaaaatcc acttggcctg ctcgggatgg aataagaatt
      601 tttccacatc cttgacggcc tgtttcaaat cgcttttggc gacttttttt tgaagcattt
      661 cgaccagctc ggcgcgattc acgaccgcaa tttttttttc ctgaagaatc gcataattcg
      721 gttcaagatt tttttgtccc aaaagccaaa ccaagtcata gaaatccctt cccttttgat
      781 acggcctttt catgatcgcc aagacttttt gggcgaaaac ggaatccagt tcgttcgcga
      841 caatgttgaa agccttgcca taggcgttca ctctttttga ttcaaatttg atatgcttgg
      901 gcggattcgg atcggcttca aatctgatca tcaatttctg tccgtccatc gggctcaagc
      961 ccatctcttt catcactgaa gaaaatttga cataaatgat gaaaatgttt ttcgttcttt
     1021 tcgctctggc gtccgctttg aagccgagtt ctttcaactt tttttcgaga tatttcgcga
     1081 gcccttcgca atcaaaaccg tttttgatca aatcaaaatc caaatcttcc gaaaatcttt
     1141 cgatattata cgcgaaacgc aggcaagttc cgccaatgaa tgacagaaat tgcccataat
     1201 cggatccata gagagctgcc aaaatttccg cttgcaaata ttccgtcaga ttttgccgcg
     1261 gtttttgggg gattccgttc tttttgatat attcttccaa aaatttctta tcgagcattt
     1321 ttttgatatt ccaacaagct gtcgattaaa atggaaactt ttttgtcgtc aaacatcttt
     1381 ttgtattcct cgattctttt tttgttgaac ttgaattctt cataaattct caattcccgc
     1441 caaaaatcga ctttcggaat caatttgttt ttgttgaaat aaatataatc cataatcgct
     1501 ttttccggca cggccagatt gaaagaaaca tttttttcaa caagcggccg atatccgatg
     1561 aagagctccg gtttgatttt ttgataatag tagtcaccgt aatcattgct gaatttcacg
     1621 ggctttcttg tcgtcaccga agtatacgcc caagcgacat caggaatgat cccatgatga
     1681 tagagagcgt attcgaggct cacatacgac ggatcataga tttttgaagc aaaagccatc
     1741 ggatcgattt cccgcgcgga gttcaccaag acataaaccc cttttctcaa tttccggata
     1801 tgcccctttt tctcccaaaa agcgatttga gacaaaagat ttttttctcc aaaaaaaata
     1861 ttcttcacct cggaagaagt aaaaaccgga taatccatca tttttttctc gaagtcgaaa
     1921 aaggtcatta taagccaaat tatatgatta tgcgtttctc tgcatttata tatattataa
     1981 tgcacggaaa cgcatctgta tgataccaga agcccggatt ttgtcaaata tgcaaatttt
     2041 ccatccaaca attgaatttt atcaaaaaat ggaatattat tgaagggtaa tctttgagca
     2101 gtccgaaaaa tggtcgaatt caggaaaata agcatcgttg ttccggtgta taatagcagg
     2161 taatctgaca tatctatata tttcaagcac accttaaaaa agcgcataaa ataacttttt
     2221 tttaaggtat ggctgaaata tatccctttt agctaaatct gtctttttat accgaagtag
     2281 aaaaatattg acacattatt ggagttgggt gtaaaacgga ggcaaatcta aaaataattt
     2341 tgttgctgtt gctcgcgata aagacacaaa taactaaaaa gcctgcttcg ctttgcgtcg
     2401 caggcttttt agttttgtga tttcgcttca tgcaaaattt tgcatttcgc ttctatttca
     2461 tgcgctgaaa ctttatcagc aatttcttga atttcatatc tcttgtctat tgtttttata
     2521 tcataagcaa aataagataa tatgggacat aatatattgt ttttccgctt attgtcttgc
     2581 gattgatttc attttttgtc acaacgattc caaaattcaa ttcgtgcttt ttcacgtagt
     2641 ccgctaaaaa catcgcgtct tttgacgtaa tcttgtttga aaatttgacc tctatcggca
     2701 attgatcgtc ttttttgttc accaaaaaat caatctcctt ttcaccctgg cgccaaaaac
     2761 tcaaaatatt gtcgattttg ttgcttttat atttttgatg cagaatattg tataccgcgt
     2821 tctcgattat tttgccgaaa gcctgtggaa cctccgaaac gtgatcagca ttgaaatgat
     2881 tcagcgcgca aacaaaattg acgcatggcg tataaatctt ttttagcatt ctgccccgtt
     2941 tgatcagact tttgtgatgt ctgtaaagaa cctccgcgat atagccctct ttgagatatt
     3001 ctatgtattt ttcgagagtc aggaatgaca aaccgacttc tctgccgata tttttgagtt
     3061 cgaatatcga accggcgttg tttatcaact gataggcaat cagcttgaac tcatcggttt
     3121 tttcaacttt gaaaatgcgg acgcagtcat ccagcacttt tccaacgact gattcagtta
     3181 tgtattcatg ttttttttca atatcggaaa tcttgcgcgt ttccggaaat tgcccgactg
     3241 ttatgtattc tcggcttagt cgggcaatat ccctgccaaa attggaatca tagacaccca
     3301 gctcgccgaa ccgtttcggc aattcaaaca aatcaaattt tttgaaaatt ttgaccgctt
     3361 catggtttat tgtcaaaaac tcgcggaaag aaagcggggg cagataaaaa tcaaaaattc
     3421 tgccagccaa actttcacta tatttgccct tgagcaaaac actttgcgat cccgtgacaa
     3481 caaattttat ttttttgcct gacagatcat agaatttttt caacaccgac tgccaatctt
     3541 caatatattg gatttcatcc agaaaaacat acacctgttc atcaagagaa aggttcggat
     3601 tgttcagaat atcttgaaaa tacgcggaca gcacctcgtc caaaaattcc gcctttttta
     3661 tttgattgga ataatcaaaa agaaaataga aaatattttt tgggtttttc tttttgagca
     3721 aaagtcggcc gatgatttgt ttcaaaatcg tgcttttccc gactcgcctc aaaccaacga
     3781 tattgagaat caaggaatgc tccatgttgg cgacaatatc atcatagatt tctctttttt
     3841 cccaatctgt ctcctctatt ttatattgat tatcactcca ccaggcattt ttcaatgcca
     3901 aatcttggat ttttatcata tttgattaaa ttagactata actgaattac gatttgagta
     3961 tatcgcattt aaatcatatt gtcaacaacg attttccgac atttgaattt tatcagaaaa
     4021 tgggatatta tagaagagta atgtttgaat agtccgaaaa tatgcccgaa ttcaggaaaa
     4081 tcagcatcgt tgtgccgata tacaacgaag aaaagactct tggcaagatc atcgccgcgg
     4141 tggaaaacgc ggatgttttt gatttggaaa aagaaattat tcttgtgaat gactgctcaa
     4201 aagacaaatc gaaagaaatt ttggaaaaat acgcgggcaa gcacaaagtt ctgcatcaca
     4261 aagtgaacca aggcaaaggc gccgccctca aaacggggtt tgccagcgcg acgggagaca
     4321 tcgtcctcat ccaggacgcg gatctggaat atgatccgaa tgaatatgtc aatcttttga
     4381 aacccattct cgacggcaaa gcggacgtcg ttttcggctc
//
LOCUS       DATKGS010000135         3044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_66743, whole genome shotgun sequence.
ACCESSION   DATKGS010000135 DATKGS010000000
VERSION     DATKGS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66743"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VMQ48_03385"
     CDS             <1..281
                     /locus_tag="VMQ48_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09104.1"
                     /translation="IARGLAWIGKNNAYIFRKVNLKKYVKENLAGGKLGGRIKDKTLH
                     SPQMDFETKLKFIPEKLYTKKAQQIGKERLKYTKDFLIRLEREIIAKL"
     gene            311..1330
                     /locus_tag="VMQ48_03390"
     CDS             311..1330
                     /locus_tag="VMQ48_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUD09105.1"
                     /translation="MKFTRTKKLLFANNKGGVGKTTLAFNCAMYFAKQGYKTVLVDLD
                     PQCNLSRLALGDNQYEKTLFSSTEKDIHDVLKGVIEGGSDIDLSVPFIPVSNSDGNLS
                     LMKGSVNLSLYENLLVTAYGQAAAGQQLGYFQTSAIDRFMREKGLKDEVDIFVIDTSP
                     SLALLNQIIFLGADYFAVPMMPDAFSVQGIENLGSIFEKWKQNWKVTGKALSGNTESK
                     FVLSGDGLFIGYIVNSYNVYSKQPIKDHRHWIEEIPKKVKKYLSEKHGRNGLVEESWK
                     NPLAEIQDYGRIPAKCQEIGVAIFDLDPALVEDIHIGTKDNIEKSKEEFKILSDRILK
                     ILAEY"
     gene            1459..1680
                     /gene="infA"
                     /locus_tag="VMQ48_03395"
     CDS             1459..1680
                     /gene="infA"
                     /locus_tag="VMQ48_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HUD09106.1"
                     /translation="MQQKDKEIIKLDGVVKETLPSTTFKVELENGHEILAHISGRMRV
                     NYIRLLPGDRVSVEMSPYDLTKGRIVKRL"
     gene            1677..1790
                     /gene="rpmJ"
                     /locus_tag="VMQ48_03400"
     CDS             1677..1790
                     /gene="rpmJ"
                     /locus_tag="VMQ48_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HUD09107.1"
                     /translation="MKVRASVKKICQKCKIVRRKGKLFVICSNPKHKQRQG"
     gene            1793..2182
                     /gene="rpsM"
                     /locus_tag="VMQ48_03405"
     CDS             1793..2182
                     /gene="rpsM"
                     /locus_tag="VMQ48_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HUD09108.1"
                     /translation="MARIAGVSIPDNKRIVIALTYIYGVGPSRSKEVLAALKLSPDTR
                     TKDLSETDVNKLKDYIEKNFTIEGELKHQKQMNIRRLKDIGCYRGIRHMKGLTVRGQR
                     TRTNTRTVRGNVRKTAGSGRRKAAEKT"
     gene            2409..2786
                     /gene="rpsK"
                     /locus_tag="VMQ48_03410"
     CDS             2409..2786
                     /gene="rpsK"
                     /locus_tag="VMQ48_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HUD09109.1"
                     /translation="MKKKKKKLRRQVLKGQAHIQCTYNNTLLTFSDMSGKVLAWASAG
                     SLGFKGAKKATPYAATQIVNAASEKVSKYGLREIEVFVKGVGSGREASIRALMNNDFE
                     IMLIKDITPIPHNGCRPRKPRRV"
     gene            2802..>3044
                     /locus_tag="VMQ48_03415"
     CDS             2802..>3044
                     /locus_tag="VMQ48_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HUD09110.1"
                     /translation="MTKLSAKCKLCRRAGEKLFLKGDRCGTPKCAIVRKPAPPGMHGA
                     KSRRGNKSEYGQQLAMKQRIKRIYAVLERQLNKYFQE"
BASE COUNT         1052 a          567 c          725 g          700 t
ORIGIN      
        1 gaattgcgcg gggtttggct tggattggaa aaaacaacgc gtatattttc aggaaagtaa
       61 atctaaaaaa atacgtcaaa gaaaatttag caggcggcaa actcggcggt cgcatcaaag
      121 ataaaacact gcacagtccg caaatggatt ttgagacaaa gctgaaattt attcctgaaa
      181 aattatatac aaaaaaagcg caacaaatcg gaaaagaaag attaaaatac acaaaagatt
      241 ttttaatccg gctggaaaga gagattatag caaagttgta aaataaaacc cttttgatat
      301 aataaaaaca atgaaattta ctcggacaaa aaaacttctt ttcgccaata ataaaggcgg
      361 tgtaggcaaa acgacgctgg ctttcaattg cgcgatgtat ttcgcaaaac aagggtacaa
      421 aaccgtactg gttgaccttg atccgcaatg caatttgtct cgcttggctt tgggggacaa
      481 tcaatatgaa aaaaccctat tttcttcaac tgaaaaagat atccatgatg tcttgaaagg
      541 agttattgaa ggtggcagcg atattgattt gagcgtgcct ttcattccgg tttcaaatag
      601 cgacggaaac ctttctttga tgaaggggag cgtcaatctt tctctttatg agaatttgtt
      661 ggtgacagcc tatggacaag cggccgccgg tcagcagctt ggttattttc agaccagcgc
      721 gatcgacaga tttatgcggg agaaaggatt gaaagacgag gtggacatct ttgtaattga
      781 tacttctccc agtcttgcac tgctcaatca aattattttt ctcggggctg attatttcgc
      841 ggtgccgatg atgccggacg cgtttagtgt gcagggaatt gaaaatcttg gcagtatatt
      901 tgaaaaatgg aaacagaatt ggaaggtgac aggcaaggcg ctttctggca acacggaaag
      961 caagttcgta ctttcggggg atgggctttt catcggatat atcgtgaatt cgtacaacgt
     1021 ttacagcaag cagccgatca aagatcatcg acattggatc gaggagattc cgaaaaaagt
     1081 aaaaaaatat ctttccgaaa agcatggaag aaatggtctt gttgaagaga gttggaaaaa
     1141 cccattggct gaaatacaag attacggacg aattccggca aaatgccaag aaattggtgt
     1201 ggcaatcttc gatctcgacc ctgcattggt agaggatatc catatcggga cgaaagacaa
     1261 tatagagaaa tcgaaagaag aattcaagat actttcagat agaattttga agatacttgc
     1321 cgaatactaa ctaagtattg acaaaaaata ctgacagttt ataatgattt cagagttaaa
     1381 aacagatatt caagcccttg gattgatacg ctccggagat aatgcaatct gagggggctt
     1441 cggccttcac ttttatttat gcaacaaaaa gacaaggaaa taatcaaact cgacggggtt
     1501 gtcaaagaaa cccttcccag taccaccttc aaagtggaac ttgaaaacgg gcatgaaatc
     1561 ctggctcata tttccggccg gatgagggtg aattatatcc gccttcttcc cggcgacaga
     1621 gtttccgttg aaatgagtcc ctatgacctc acgaagggtc gtattgtaaa aagactatga
     1681 aagtaagagc atcagtcaaa aaaatttgcc aaaagtgcaa aatagtccgg cgaaaaggaa
     1741 agcttttcgt gatttgttca aatccaaaac ataagcagcg tcaaggataa atatggcgag
     1801 aattgcaggg gtaagcatcc ccgacaacaa aagaatagtg atcgccctca cctatatata
     1861 cggtgttggc ccttcgagga gcaaagaagt gctcgcggct ttgaaattga gccctgacac
     1921 gagaaccaaa gatctttccg aaacagatgt gaacaaactc aaggattata ttgaaaaaaa
     1981 tttcaccatt gaaggagaat tgaagcacca aaagcagatg aatatccggc ggctcaaaga
     2041 cattggttgt tatcggggaa tccgccatat gaaagggctc acggtgcgcg gacagcgaac
     2101 ccggacgaat acccgaactg tgcgtggaaa tgtgagaaag acagccggaa gcggacgacg
     2161 caaggcggca gaaaagacgt aaaaataatt caaaatgcaa aaattaaaag tcaaaatgac
     2221 agtgcaaaat gtaaaattgt attgaactca aagatacctt tatataatta gctataatgg
     2281 ccgaagaatt aaagaaaact gaggaaaaag agcaaaaaaa tgacgaaaag tcagaagttg
     2341 tttctgtcga taaaaaagaa gaaaaaactg cagatgcaac cgaatcaacc gatgaaaaag
     2401 cggcggaagt gaaaaaaaag aagaaaaaat tgcggcggca ggtccttaag gggcaggccc
     2461 atatccaatg cacatacaac aataccctgc tgactttttc ggatatgagc ggaaaagtgt
     2521 tggcgtgggc aagcgccggt tctcttgggt ttaagggggc gaaaaaggcg acgccgtatg
     2581 ccgcgacgca gattgtgaat gccgcttcag aaaaagtctc caaatatggg ttgcgagaga
     2641 tcgaagtttt tgtgaaaggg gttggaagcg gacgggaagc ctcaatccgg gctctcatga
     2701 ataatgattt tgagataatg cttatcaaag acattactcc tattcctcac aacggttgca
     2761 ggccgcgaaa gccgaggcga gtataataat atagcaaaaa gatgaccaaa ctatccgcaa
     2821 aatgcaaatt gtgccggcgc gccggagaaa aacttttttt gaaaggtgac cgttgcggca
     2881 cgccaaaatg cgctattgtc cgcaaaccag ctccgccggg aatgcatggc gccaagagcc
     2941 gcagaggaaa caaaagcgaa tatggccagc agttggccat gaaacagagg atcaagcgaa
     3001 tctatgccgt tttggagcgc caattgaaca agtatttcca agaa
//
LOCUS       DATKGS010000136         3388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_198676, whole genome shotgun sequence.
ACCESSION   DATKGS010000136 DATKGS010000000
VERSION     DATKGS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198676"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="holA"
                     /locus_tag="VMQ48_03420"
     CDS             complement(<1..841)
                     /gene="holA"
                     /locus_tag="VMQ48_03420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HUD09111.1"
                     /translation="MIIFLYGEDEFRSMEKLAGIKNKFLEKNKEGVSLSVFDFAEKDW
                     NIGEIIMNISSGGLFTSKKLAIIKNILAGKKESSDDIFEKFLKNEAKKEKSDLILIFW
                     ESGKTKKSSKLYKLLKKITKCQEFELLEGAKLRNWVIGEFKKSDITINSAAVEKLVVF
                     VGNDLNLLSREIEKLATYKSKGEITGGDIDLLVKSKIDTDIFKTVDALARGDKKTALK
                     LLHDHLEDGEDPFYLLSMYFYQFRNLVKVKPLAEKNISPHEIASKLKMHPFVARKSLE
                     QASS"
     gene            complement(892..2016)
                     /locus_tag="VMQ48_03425"
     CDS             complement(892..2016)
                     /locus_tag="VMQ48_03425"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HUD09112.1"
                     /translation="MLDRIKEMSTKTKIITVVVIIILLVGVGYWRGWFGGKNKVNPLN
                     LNAPSGESSAPLNTGNVSPFSGLACANWNRRPIAVMEAADVSVRPDSGFSDADLVLEM
                     PAITASITRLMPVYVCNDPPEVGSIRSTRHDYIALAAGWDAILAHWGGSHFAADILNK
                     GTLGKGGVVDDLDLNGSMGHKAPECGFRKEGIPAPDNGFAKFPTLLDCAKKAGYDMTD
                     KFSGYPHQAEATPDQRPKGGELRVAFAKPYDVNYTYDPASNTYLRTWGGTADVDANNG
                     KRNAPKNVIVVIASSEQIEGQYNNVQIGDPQFDTVTEGDAHYYLNGQEFAGRWKKDKS
                     SWSSKLFLYDDKGQEMKFVPGQMWVEVLEPGQGLKWTPAS"
     gene            complement(2036..2899)
                     /gene="mutM"
                     /locus_tag="VMQ48_03430"
     CDS             complement(2036..2899)
                     /gene="mutM"
                     /locus_tag="VMQ48_03430"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HUD09113.1"
                     /translation="MPELPEVQTIVDDLNRKIKGDVIVGFWSNWKKTVRMPVENFEKE
                     IKGKKILSASRIGKNIFINLSGGKTIYIHLKMTGHLLIKSKIKNPKSKADGDDYFSEK
                     VNQYIHHIFYLKSGKTLEFSDLRKFGKIILADTGKIKDIKEIGSLGIDAMSKEFTFEK
                     LNEILDKRATKIKLLLMDQNKLAGVGNIYASEILFEAGILPMRPAYKISREERKKLFL
                     AIKKTLEKGIKLRGTSDSDYRDTAGAPGGFQKVLKVYRRAGKTCGKCGTIVQRIKLGQ
                     RGTFFCPVCQK"
     gene            complement(2933..>3388)
                     /locus_tag="VMQ48_03435"
     CDS             complement(2933..>3388)
                     /locus_tag="VMQ48_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09114.1"
                     /translation="IMIAIFAVFASYQFVSLHSADRGIGLLPGNEAAGEFLKSEKIAG
                     PIFNNYDIGGYLIWNLPASEKVFVDNRPEAYPDSFFSEIYKPMQENPDVFKKVDAQYN
                     FNTVVFSRNDITPWGMNFLKMIKDDPNWAPIFEDNFAIIYTKKGDKEIR"
BASE COUNT          821 a          857 c          641 g         1069 t
ORIGIN      
        1 gggaggaggc ttgctcgagg cttttccgcg caacaaaagg atgcattttc aattttgatg
       61 cgatttcgtg cggcgaaata tttttttcgg ccagcggttt cactttcacc aaatttctga
      121 actgataaaa atacatcgaa agcaggtaaa atggatcctc accgtcttcg agatggtcgt
      181 ggagtaactt caaggctgtt tttttgtctc ctctcgcgag cgcatcaaca gttttgaaaa
      241 tatccgtgtc gattttcgat ttcacaagca gatcaatatc cccgcctgtg atttcccctt
      301 tgcttttgta cgtcgccagt ttttcaattt cccttgaaag caaattcaga tcatttccga
      361 caaaaacaac cagtttttca actgctgccg aattgatagt gatatcgctt ttcttgaatt
      421 ccccgatcac ccagtttctc aattttgctc cttccagaag ttcaaattcc tggcacttcg
      481 taattttttt gagcagttta tatagcttac tgctcttttt cgtctttcca ctttcccaaa
      541 atatcaaaat caaatcactc ttttcttttt tggcttcatt tttcaaaaat ttctcgaaaa
      601 tatcatccga agattctttt tttccggcca gaatattttt gatgatcgcc aattttttgg
      661 aagtgaacaa accgcctgaa gaaatattca taatgatttc tccgatattc caatcttttt
      721 ccgcaaaatc aaaaacagag agggacacgc cctctttgtt tttttccaga aatttgtttt
      781 ttatcccggc gagtttttcc atcgaccgaa attcgtcctc gccatacaga aatataatca
      841 ttgcaaatcc agaccaaaaa atactttata ctttctacta aatacttgat actagctcgc
      901 cggtgtccat ttcagcccct gtcccggctc aagaacttcc acccacattt gccccggcac
      961 gaatttcatt tcctgcccct tgtcgtcata gaggaagagc ttgcttgacc agctggattt
     1021 gtcttttttc cagcgtcccg cgaattcctg tccgttgaga tagtagtgag catcaccttc
     1081 cgtcaccgtg tcaaactgcg gatcaccgat ctggacgttg ttatattggc cttcgatctg
     1141 ttcagaggag gcgatcacaa cgatcacgtt tttcggcgcg tttctttttc cattgttcgc
     1201 atctacatcg gccgttcctc cccaggtgcg cagatatgtg tttgatgccg gatcgtaggt
     1261 gtagttcacg tcataaggct tggcaaacgc aactcggagt tccccgcctt tgggcctctg
     1321 atcgggcgtt gcttcggctt ggtgcggata tccggaaaat ttgtccgtca tatcatagcc
     1381 ggcttttttc gcgcaatcaa gaagcgttgg aaatttggca aatccgttgt ccggtgcggg
     1441 aattccttct tttcggaatc cgcattccgg cgctttgtgt cccatgctgc cgttgagatc
     1501 caagtcatct accactccgc ctttgcccaa agttcccttg ttgagaatat ccgccgcgaa
     1561 atgtgaaccg ccccaatggg ccagaatcgc gtcccatccg gccgcaagag cgatatagtc
     1621 atgccttgtg gaacggattg atccgacttc tggaggatca ttgcagacat acaccggcat
     1681 caagcgggtg atgctggccg tgatcgccgg catttcaagc accagatctg cgtcagaaaa
     1741 tccgctgtcc ggacgcacgg aaacatccgc cgcttccatc acggcaatcg gccgacgatt
     1801 ccagttggcg caagccaggc ctgaaaaggg actcacgttt cccgtattca agggcgcgct
     1861 gctttcgccg ctcggcgcat tgaggttgag cggattcact ttgttttttc cgccgaacca
     1921 tccgcgccaa tatccaacac caacaagcag gattattatc actacaacag tgataatttt
     1981 ggtcttggtc gacatctctt tgattctgtc caacatactt tttctctata aattattatt
     2041 tctggcaaac tggacagaaa aaagttcctc tctgtcccag ttttatcctc tggactattg
     2101 taccacattt cccgcatgtt tttccagccc ggcgatagac tttcaaaact ttctgaaatc
     2161 ctcccggcgc tccggccgta tcacgataat cgctgtcaga tgttcctcgc aattttatcc
     2221 ctttttcaag cgtttttttg atcgccaaaa acaatttctt ccgctcctcg cgcgatatct
     2281 tgtatgccgg acgcattggc aaaatgccgg cttcaaacaa tatttcgctc gcatagatat
     2341 ttccaacccc ggcgagcttg ttttgatcca tcagaagcaa tttgatcttc gtcgcgcgct
     2401 tgtcgagaat ctcattcagt ttttcaaacg tgaattcttt gctcatcgca tcaattccca
     2461 agctgccgat ttcttttata tctttgatct tgcccgtatc cgctaaaatt attttcccga
     2521 atttacgaag atcggaaaat tccagcgtct tcccggattt gagataaaag atatgatgga
     2581 tatattgatt tactttttct gaaaaataat catccccgtc ggctttggat tttggatttt
     2641 tgattttgga ttttatcaat agatgtcccg tcatcttcaa atggatatat attgttttcc
     2701 ctcccgacaa attgatgaag atattttttc caatccggct ggcgctcaat atttttttgc
     2761 ctttaatttc tttttcaaaa ttttcaaccg gcattctgac ggtctttttc caattactcc
     2821 aaaaaccgac aatcacgtca cctttgattt tcctgttcaa atcatcgaca attgtttgga
     2881 cttcgggcaa ttccggcata aatttatttc ctaattttcc aatcccccta atttaccgta
     2941 tttccttatc tcccttttta gtataaatga tcgcaaaatt atcctcaaag atcggcgccc
     3001 aattcggatc gtctttgatc attttcaaaa aattcatccc ccacggggtg atgtcgttcc
     3061 gggaaaaaac aaccgtgttg aaattatact gcgcatcaac ctttttgaaa acatccggat
     3121 tttcctgcat cggtttgtat atttcgctga aaaatgaatc ggggtatgct tccggacgat
     3181 tgtcgacaaa aactttttcg ctcgcaggca agttccaaat gagatagccg ccaatatcat
     3241 agttgttgaa aatcggaccg gcaatttttt cgctcttcaa aaattcccct gccgcctcat
     3301 tcccaggcaa aagaccgatc ccgcgatcag ctgaatggag ggaaacaaac tgatagctcg
     3361 caaaaacggc aaaaatcgcg atcatgat
//
LOCUS       DATKGS010000137         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_198708, whole genome shotgun sequence.
ACCESSION   DATKGS010000137 DATKGS010000000
VERSION     DATKGS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198708"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VMQ48_03440"
     CDS             <1..495
                     /locus_tag="VMQ48_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HUD09115.1"
                     /translation="GDTFHEKKAVEVGNIFKLGTKYSAPFDLTVMNEKGEKTPVIMGC
                     YGIGLQRLMGTLVELCSDENGILWPESVAPFRVHLISLGKNEEAEKIYKDLEKKNIEV
                     LFDDREDTSAGAKFADADLIGIPWRVVVSQKSLAGGGVEVKKRNEKESEIIKIEELLK
                     ILKK"
     gene            618..1286
                     /locus_tag="VMQ48_03445"
     CDS             618..1286
                     /locus_tag="VMQ48_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09116.1"
                     /translation="MDAQETPKENQNITLDKKSKILFVIIGLLIIGSVAVTFWRYMVK
                     RDYIVQAQTDCDPTTEKCFIWKCDPASTVEGEKCTGDPDKDTWYYKIIHRNAKNIPLC
                     DPNDKNCKALICDPGEKDCSEELCTAQNVKAPDTCNDPAQYNIDNPPEEDTSTECAPD
                     DQACLDAQGLEDAGEECAPDDQECLDAQASEDTKETCSPDDPTCDQTQDSTTSPDGSD
                     ATTP"
     gene            1512..2351
                     /gene="hisG"
                     /locus_tag="VMQ48_03450"
     CDS             1512..2351
                     /gene="hisG"
                     /locus_tag="VMQ48_03450"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HUD09117.1"
                     /translation="MKNVALAIPNGSLFEKTAQLLKKAGAIFHFGKRQFETSVEGLGI
                     FNHIVVMRPQAIPLSVKRRIAAAGICGWDCVVESGLEKSLELVTELQYSKATPSPVRI
                     VVFGKKEKLVDSSRTQVLTEYPNLAKQVFKKATLDFSHGSTEVQILSGAYDFGVGITE
                     TGESLRANGLKIVKTILVSPTVIVARESTPELNIFGEIMKGAIDAEKYQLVKMNADQK
                     AKEKILEILPAMKSPTINELANGSFSIETVIGKSSLVSVLMDLRHLGATDILVQDVNI
                     VLA"
     gene            complement(2603..>2809)
                     /locus_tag="VMQ48_03455"
     CDS             complement(2603..>2809)
                     /locus_tag="VMQ48_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09118.1"
                     /translation="NFFANSFILPLPFKELFSTMLSEGFFETKKLPVLARNHKNCAKG
                     RRVLKNFVVPPFFRYQKTPGKAPT"
BASE COUNT          878 a          611 c          674 g          646 t
ORIGIN      
        1 ggggatacct ttcacgaaaa aaaagcggtg gaagttggga atattttcaa gttggggaca
       61 aaatacagcg cgccgtttga tctcaccgtc atgaacgaaa aaggcgaaaa aacgccagtc
      121 attatgggct gctatggaat cggcctccag cggctgatgg ggactcttgt ggagctctgc
      181 agtgatgaaa acggcatcct ctggccggag tccgtcgcgc cttttcgagt ccatctcata
      241 tcacttggaa aaaacgaaga ggcggaaaag atatataagg atcttgagaa aaaaaatatc
      301 gaagttttgt tcgatgatcg cgaagacacc agcgccggag ccaaattcgc cgacgcggat
      361 ctgatcggca ttccgtggcg cgtggtggtc agtcaaaaat cccttgccgg tggaggagtg
      421 gaagtgaaaa aacgtaatga aaaagaaagc gaaataatta aaatcgaaga attattaaaa
      481 atacttaaaa aataaatctc aaaaaacctc ctcccctgcc aaggggaggt gcccgaaggg
      541 cggaggggtt ttcaatcctg caaagaaaac cacccctctt atcccctcct tggcaggagg
      601 ggaggatcga taaaattatg gacgcccaag aaacaccaaa agaaaatcaa aatataactc
      661 tcgacaaaaa atccaaaata ctgttcgtga tcatcggact tttgatcatt ggatcggtcg
      721 cggtgacttt ttggcgctat atggtcaagc gcgactatat cgtccaagcc cagactgatt
      781 gcgacccgac aacggaaaaa tgtttcatct ggaaatgcga tcccgcttca acagtggaag
      841 gcgaaaaatg cacgggcgat ccggacaaag acacttggta ctataaaata atccatcgca
      901 acgccaaaaa tatccctctt tgcgacccga acgacaaaaa ttgcaaagcc ctcatctgcg
      961 atccgggcga aaaagactgt tccgaagagc tgtgtactgc gcagaatgtg aaagcacccg
     1021 acacttgcaa tgatcccgcg caatacaaca tcgacaatcc accggaagaa gacaccagca
     1081 ccgaatgcgc gccagacgat caggcgtgcc tcgacgcgca aggtttagag gacgcaggag
     1141 aagagtgcgc tccggatgat caggagtgcc tggacgcaca ggcttcagaa gataccaaag
     1201 aaacctgctc ccccgatgat ccgacttgtg atcaaacgca agattcaacc acaagccctg
     1261 atggctcgga cgcgacaacg ccatagcttg acaaaaatat tcaatccatg ttaattttct
     1321 gtgtagtaaa agttataaac caatcccaat gatcaaaagt agattgtata caataaatat
     1381 ccatcataat ccttctttat tttcaagaag gaacgagttt tgattttttc tactgacttt
     1441 ataatcacga ttttatcctt cttaaaaaga gggatttttt gttgtttaaa aaaccaataa
     1501 ggagaaagaa aatgaaaaac gtggcgttgg caattccgaa tggaagtctc tttgaaaaaa
     1561 ccgcccagct tttgaaaaaa gcaggagcaa tttttcactt tgggaaaagg caatttgaaa
     1621 ccagtgttga aggtttgggc atctttaacc acatcgttgt tatgcggccg caggcgatac
     1681 ccttgtcggt gaaaaggagg attgccgccg ccggaatttg cggatgggat tgtgtcgttg
     1741 aatccgggct ggaaaaatca cttgagctgg tgacggagct gcaatacagc aaagcaactc
     1801 caagtccagt acggattgtc gtattcggaa aaaaagagaa actcgttgat tcttcacgca
     1861 cacaagttct caccgaatat ccaaatctgg caaaacaggt tttcaaaaaa gccacgttgg
     1921 atttctcgca cggtagcacg gaagtccaaa ttctgtccgg cgcgtacgat tttggcgtcg
     1981 gcatcaccga aacaggagaa agcctccggg ccaatggact gaaaatcgtg aaaaccatct
     2041 tggtttctcc gacggtcatc gtggcaaggg aatcaacacc cgaactgaat atattcggcg
     2101 agatcatgaa aggagcgatc gatgccgaga aatatcagct cgtcaaaatg aatgccgatc
     2161 aaaaagccaa ggaaaaaatc ctggaaattt tgccggcgat gaaatcaccg acaatcaatg
     2221 aattagcaaa cggttctttt tcaatcgaaa ccgtaatcgg aaaaagttca ttggtcagcg
     2281 tactgatgga tttgcgtcat ctcggcgcaa ccgacatctt ggtgcaggat gtgaacatcg
     2341 ttctggcata acaatcaaag cgcggctcat tgagtcgcgc tttttcataa acaatgggct
     2401 tgacaaacat tttgccacaa tatatattgg cagtagctta acaataacaa atcccaagtt
     2461 cgggtcccga ttgaaaaatc gggattgggc tcgaacaatc ctgatttgca ggatagggag
     2521 gtttctgcgt gagtggaagc ccggtttccc ccgtaatcat gggataatcg atgtcggcga
     2581 aagtcggagt cgatgagcgg aatcaagttg gcgcctttcc gggcgttttt tgataccgaa
     2641 aaaagggtgg taccacgaag tttttcaaga ctcttcgtcc ttttgcgcaa tttttgtgat
     2701 tgcgcgcgag gacggggagt ttttttgttt cgaaaaaacc ctcgctcaac atcgttgaga
     2761 aaagttcttt aaaagggaga ggcaagatga aggaatttgc aaagaaatt
//
LOCUS       DATKGS010000138         4510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_184458, whole genome shotgun sequence.
ACCESSION   DATKGS010000138 DATKGS010000000
VERSION     DATKGS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184458"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /gene="pnp"
                     /locus_tag="VMQ48_03460"
     CDS             <1..2131
                     /gene="pnp"
                     /locus_tag="VMQ48_03460"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HUD09119.1"
                     /translation="GSTGKNSSMEVKKFETEIAGRKLVVETGLLAQQANAAVTVRYGD
                     TVVLATAVMSPNAREGVNYFPLMVDYEEKFYASGKIKGSRFIKREGRPTDEAVLNGRL
                     IDRTLRPLFDQQMRNEVQVIVTVLSIDQENDPALVGAIAASTALSISDIPWNGPIGAV
                     RIGEKEGKVVINPTNGDNAASSYNIFACGVEGKINMIETGANEVAKEKLFDAFVEGQK
                     VIDQIVVFIKDIQKEIGKPKSEALLIAPDAEFKKEIIEKFADKIAPALYQKDHAVQTK
                     LQNELFAEAAQYVKEKYPIEANAKKELAFAVIDSEMENIFKKSVLEKDERPDGRKLNE
                     VRPISCIVGLLPRTHGSALFTRGETQALSIVTLGGPGMEQIIDTMELDEKKRFMHHYN
                     FPPFSVGEVKPMRGPGRRDIGHGALVEKALTIVAPDKDKFPYTIRTVSEVLSSNGSSS
                     MASTCASNLALLDAGVPMRSVIGGISVGIVVGPDGKYKLLSDIQGPEDHFGGMDFKIA
                     GSKKGITAIQLDVKVDGLTADMIRDTLEQSEKNRIAIIDIMNETIDAPRAEMSQYAPR
                     IIILKINPEKIRDVIGPGGKIINQIIDETGVEIDIEDDGSVFITAASPEAGKKASEWV
                     QNLTHEVTAGETFKGKITRILNFGAFAEILPGQEGLIHISEISEKRTERVEDALKVGQ
                     TVNVKVKEIDSLGRINLTMRGLENSVE"
     gene            2163..3320
                     /locus_tag="VMQ48_03465"
     CDS             2163..3320
                     /locus_tag="VMQ48_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09120.1"
                     /translation="MFQFKNTGLGMKEPDTSNALPNTFGKVRTFKEDLENFEKGTPVK
                     DASEEVAELPAEDKKPIVENLPQGQALPQATPAAPAVEYPPGSPFQSAPTPPPISSPS
                     PGANLDTQLPSFRSSPSQSFFAEKPAPEENPSPEHAENQSLPQKKSKGGLMLMLAVVL
                     VVAAAGAGFYYYWFHIKSNSSETSSNPTAPTPPVASPAPAQSQNKNLRNLVVDTSQGS
                     TEMKNAIQKFATDFSTSASENDLVEVKIIGKDNQPVGKKDFFSGVGVTVPDPVLMKLS
                     EDYSLFARKEGGAVRLGFVFKTVTSSGLADEMKNWEPTLPTDLTPLYVGQAPTGMGPF
                     NSSLYKNADIRYFNFLSPADTSLDYSVISNFLVIGSSKDTTRAVLDYMSQK"
     gene            3943..4338
                     /locus_tag="VMQ48_03470"
     CDS             3943..4338
                     /locus_tag="VMQ48_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HUD09121.1"
                     /translation="MAKNTLQKEIGNLGERIAANYFLARGCEILEMNFENKFGYRLGE
                     LDVVARDLKTNEIIFAEVKTRKKGTSNSESPELAISRAKYRKLSRIISNYLRKNNMLD
                     CDYRLDAIAIELDMQTRKASLRHLKCIYY"
BASE COUNT         1358 a         1055 c         1033 g         1064 t
ORIGIN      
        1 agggagtact ggaaaaaact cttcaatgga agtgaaaaaa tttgagacag aaattgccgg
       61 aagaaaactc gttgtcgaaa cgggtctttt ggcgcagcaa gccaacgcag ccgtgactgt
      121 ccgctacggc gacacggttg tcctcgcaac agcagtgatg agccccaacg cgagggaagg
      181 tgtgaactat ttcccgctga tggttgacta tgaagagaaa ttctatgcct ccgggaagat
      241 caagggtagc cgattcatca agcgcgaggg aagaccgacg gacgaggcgg tgctgaacgg
      301 ccggcttatc gaccgcacct tgcgcccgct gtttgaccag caaatgagaa acgaagtgca
      361 ggtgatcgtg accgtgcttt ccattgacca agaaaatgac ccggctcttg tcggagccat
      421 cgccgcttca acagcgcttt ccatttcgga tattccttgg aacggtccca tcggagcggt
      481 gcggatcgga gagaaagaag gcaaggtcgt tatcaatccc acaaacggag ataacgccgc
      541 cagttcctat aacattttcg cctgcggtgt tgagggaaaa ataaacatga tcgaaacagg
      601 cgcaaacgaa gtcgccaaag aaaagctttt tgacgccttt gtcgaaggac aaaaagtgat
      661 cgaccagatc gtcgttttca ttaaagatat ccaaaaagaa atcggaaagc caaaatcaga
      721 agctcttttg atcgcccccg acgccgaatt caaaaaggag attattgaaa aatttgcaga
      781 taagattgct ccggcgttgt atcagaaaga ccacgctgtc cagacaaaat tgcaaaacga
      841 actttttgcc gaagccgccc agtatgtcaa agaaaaatat cctattgaag caaacgcgaa
      901 aaaagagctt gcctttgctg tgattgattc cgaaatggaa aatattttca aaaaaagcgt
      961 tcttgaaaaa gatgaacggc cggacggaag aaaattgaat gaagttcgtc cgatttcctg
     1021 cattgtcgga cttttgccgc gaacgcacgg cagcgcgctt ttcacccgtg gagaaacaca
     1081 agccctttcg attgtgacgc tcggcggccc ggggatggag cagatcatcg acacaatgga
     1141 gctcgatgag aaaaaaagat tcatgcatca ctataatttt cctccctttt ccgtcggaga
     1201 agtgaaaccg atgcgcggac cgggacggcg cgatatcggc catggagcgc ttgttgaaaa
     1261 agcgctcacg atcgttgctc ccgataaaga taaatttccc tacacgatcc ggacagtcag
     1321 tgaggtcctt tcttcaaacg gttcgtcgtc aatggccagc acttgcgcct caaatcttgc
     1381 ccttttggat gcaggcgttc caatgaggtc agtgattgga ggaatttccg tcggcattgt
     1441 tgtcgggccg gatggaaaat ataaattgct ttccgatatc caaggtccgg aagatcattt
     1501 tggcggaatg gatttcaaaa tcgcgggatc aaaaaaggga atcactgcca tccaactgga
     1561 tgtgaaggtg gacggactca ccgccgatat gatccgcgat acgctcgaac agtctgagaa
     1621 aaatcggatt gcgatcatcg acattatgaa cgagaccatc gacgctcccc gcgccgagat
     1681 gtctcaatac gcgccaagaa tcatcatcct caaaatcaat cctgaaaaaa tccgcgatgt
     1741 gatcggaccg ggaggaaaaa tcatcaatca aattatcgat gaaaccggag tagaaatcga
     1801 cattgaggac gatggatcgg ttttcatcac cgctgcgagt cctgaagcgg gcaaaaaagc
     1861 ttccgagtgg gtacagaacc tcacccatga agtgaccgcc ggagaaacct tcaaggggaa
     1921 aataacccgc atcttgaatt tcggcgcctt tgccgagatt cttcccggac aggaaggact
     1981 catccatatt tccgaaatat cggaaaaaag aaccgagcgg gtggaggacg cgctcaaagt
     2041 cgggcagacc gtgaacgtca aagtgaagga gattgacagc ttgggcagga tcaacctcac
     2101 tatgcggggc ttggaaaata gtgtagaata aatttacact aaataatata aaaacaaacc
     2161 aaatgttcca attcaaaaac acgggattgg gaatgaagga acctgatact tcaaatgccc
     2221 ttccaaatac tttcgggaaa gtccgaacat tcaaagaaga tcttgaaaac ttcgaaaaag
     2281 gaacgcctgt caaagacgct tccgaagaag tagctgaact tcctgcggaa gacaagaaac
     2341 caatcgttga aaatcttcct caagggcaag cgttgcccca ggcaacaccg gctgctccgg
     2401 cagtggaata tcctcccggc agcccttttc agtctgctcc cacgcctccg cccatttctt
     2461 ctcccagtcc cggtgccaac cttgacaccc aactcccttc tttcagatca tcaccctccc
     2521 aatcattttt tgcggagaag ccggctcccg aagaaaatcc ttctcctgaa cacgcggaaa
     2581 atcaatctct tcctcaaaaa aaatccaaag gtggtctcat gctgatgctt gccgttgtcc
     2641 ttgttgtcgc cgccgccggt gccggttttt attattattg gtttcacata aaaagcaatt
     2701 cttccgaaac ttcttcaaat ccaaccgccc cgactcctcc ggttgcttct cctgcgcccg
     2761 ctcaatctca aaacaaaaac cttagaaatc tagtcgtcga cacgtcccag ggatcaaccg
     2821 agatgaaaaa cgctattcag aaattcgcga ccgatttttc cacctctgct tcagaaaacg
     2881 acctggtcga ggtgaaaata atcgggaaag acaaccaacc ggttgggaaa aaggattttt
     2941 tttccggtgt tggagtgacg gttccggatc cagtgctgat gaagctttct gaagactata
     3001 gcctatttgc cagaaaagaa gggggcgcag tgagattggg atttgttttc aaaactgtca
     3061 cgtcgtcagg cctcgcggat gagatgaaaa actgggagcc gaccctcccg acagatctga
     3121 ctcctctcta tgtcggtcaa gccccgaccg gcatgggacc tttcaattcg agcctttata
     3181 agaacgccga tatccgatat ttcaactttt taagcccggc ggacacatct ctcgactatt
     3241 ctgtcatctc aaatttcctg gttatcggat cctccaagga cacaaccaga gccgttctgg
     3301 attatatgtc gcagaagtaa tgaaaattga aagttcaaaa atcaaaatgc aaaatgacag
     3361 tgtaaagttg aaaatgaaca aatgttaaaa ttattttgac tttcagtaat ttttgcattt
     3421 ttcattgaaa tttttaattt ttatcttttc attttgaatt tgatttgacc tttcccgcaa
     3481 aatctgctat actgtatatg taccttgaaa agccaaaggt ttacgagttc caatcacaat
     3541 ttccccctat ttgtgattgg aactcgtaaa cttccctata ttctttcccc aacgtgggag
     3601 ggttaaaaac aaaaataaac ttatttttga aaattttaca aaaataaaaa tcaatagcag
     3661 gttctttata accgtaggga ggtgatgcga taacagacga cacgttttca aaatgaatgt
     3721 tgaccgtatg tactaattac gctttcaccc ggcgtaaagt tctcggcttt gacattctgg
     3781 gatgtgtcaa acatcgggcg ccgccgccac ttattattat ttgcgggcgc ttgcggcgcc
     3841 cgattccaga tatcttcaaa aatatttcaa tatagttcaa gtgaaacagg cagccttcca
     3901 gggcggtttt tttgttggcg agatttgcgc taatatatag gcatggcgaa aaacaccctt
     3961 caaaaagaaa ttggcaatct cggcgagcgg atcgccgcca actattttct tgcgcgggga
     4021 tgtgagattc tcgaaatgaa ttttgagaac aaatttggat acaggctggg agagctggat
     4081 gtggtcgccc gcgacctgaa aacgaatgaa ataattttcg cggaagttaa gacgagaaaa
     4141 aaaggaacga gcaattcgga atcgccggaa ctcgccatca gccgggcaaa ataccgcaag
     4201 ctttcaagaa taatttcgaa ttatctgcgg aagaacaaca tgttggactg cgactaccgc
     4261 ctcgacgcca tcgccatcga gcttgatatg cagaccagaa aagccagttt gcggcacttg
     4321 aagtgcattt attattgatg ctatactaat aaacgtctaa tactaagcta aacatatgat
     4381 cgatccacaa atccaggagg aactcaaaca aaaactgctt gaagaaaaaa aacgaatcga
     4441 agaaaacctg tccaaaacag aaaccggaaa gaaagacgct gatcgggaat atgagaccgc
     4501 ctttccggaa
//
LOCUS       DATKGS010000139         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_199087, whole genome shotgun sequence.
ACCESSION   DATKGS010000139 DATKGS010000000
VERSION     DATKGS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199087"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VMQ48_03475"
     CDS             complement(<1..352)
                     /locus_tag="VMQ48_03475"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09122.1"
                     /translation="MQVDNERLKSFALDAGLVDEQKIDAAIEESAKTGKKLGDVLVEQ
                     KLVSADQLRQLFSYILGIPFVNIEKEIIPKDILQIVPEPIAKKYKIVAFKKNGTELKV
                     AMLNPEDIQTIDFIP"
     gene            complement(353..727)
                     /locus_tag="VMQ48_03480"
     CDS             complement(353..727)
                     /locus_tag="VMQ48_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUD09123.1"
                     /translation="MDTPQKILIIEDDRALQSALVEMLSQEGYETISAYDGQEGMEKA
                     EVEKPNLILLDLILPKKDGYEVLAEIKKGSDKNIPVLILTNLEELDNVQRALDLGAKT
                     FMVKSDFSLRDIIEKIKENLQK"
     gene            complement(795..1745)
                     /locus_tag="VMQ48_03485"
     CDS             complement(795..1745)
                     /locus_tag="VMQ48_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUD09124.1"
                     /translation="MFAKFLDDFRFITTCKKYGLGLWQCPNFLFLMMGLITIVAMVGT
                     FFVSSRFASEGTTVMSVTIEVVFLMIIGSSVIRGVEKVAEANIMKTEFISIISHQLCT
                     PLSAIKWNMEVIETERDTKECLPEKQMVFLQNIKKSNEQMLKMVTDLLEVARVDQGKS
                     VFKESIFDLADLAKESIVGFQGMAIEKNVKIESQIAEGPQRVCSDEKKMRVVFDNLIG
                     NAIKYSKEGGKVEIKLKKNKEKIIFSVQDWGVGIPRYQHSKIFEKFFRSKNESRYRTD
                     GVGIGLYLVKAILGHCGGKVWFESEAGKGSIFYFSLPAQS"
     gene            complement(1879..3624)
                     /gene="groL"
                     /locus_tag="VMQ48_03490"
     CDS             complement(1879..3624)
                     /gene="groL"
                     /locus_tag="VMQ48_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HUD09125.1"
                     /translation="MAKQIRFDEDARRRIKAGIDKVADAVRVTLGPKGRNVVLDRGFG
                     SPMVTNDGVTIVKEIDLEDKFENIGAEFVKEVANKTNDAAGDGTTTATILTQSIVESG
                     AKFISKGINVIELKNALLKLSNRVSKDLISSAKVVSGDAIEQVATISAQDPEVGKMIA
                     KVIEKVGKEGVITVEESQTFGLAEEIVEGMKFDKGYISPYMITSAESMEAELKDPQIL
                     ITDKKISSVQEILPILEKIAQTGKKEMVIIAEEVEGEALTTLVVNKLRGILNVLAIKA
                     PGFGDSRKAMLEDIAILTGGQVISEERGMKLESVTLEDLGKAGRIVSTKDDTTLVDGK
                     GKKKDIDARVAQLKTQIEKATSDFDKEKLQERLGKLSGGVAVIKVGAATEVEQKEKQH
                     RVEDAVEATKAALEQGIVAGGGIALLNESVKLSADLQKNLSHEDRIATQILVDALSAP
                     IKQIVANAGQSAEVVVSRVQDLQDTFLKKTAKGKKVNIETGTEIEMEYHVNEVNPKAD
                     WTMGYNADENTYVDMLKAGIIDPAKVVISALGNAVSAAAMLLTTEAVVTDIPEKKEKG
                     GGMPDMSGMGGMGMM"
     gene            complement(3762..>4134)
                     /locus_tag="VMQ48_03495"
     CDS             complement(3762..>4134)
                     /locus_tag="VMQ48_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09126.1"
                     /translation="GRYSFIYPIFGYNIFQKFCGINLEWIKKIKPNWEIPELKPARYF
                     VDVRRLQWAVQRSFESLINLFWGDRIESWLKRHQITRIERNPLTKKEGAYIEYTDKNL
                     IFLPEPQGVRVEEEWQRRVNP"
BASE COUNT          960 a         1083 c          793 g         1298 t
ORIGIN      
        1 gggggatgaa atcaatcgtt tgaatatctt ccggattgag catggccacc ttgagctctg
       61 ttccgttttt tttgaaagcg acgatcttat atttcttcgc gatcggctcg ggaacaatct
      121 gaagaatatc cttcgggata atttcttttt cgatgttcac gaacggaata cccaggatat
      181 atgaaaaaag ctggcgcagt tggtccgcgc ttaccaattt ttgctcaacc aaaacatcgc
      241 ccagtttttt cccggttttg gcgctttctt cgattgccgc gtcaattttc tgctcatcca
      301 cgagccccgc gtccagggca aaacttttga gccgttcgtt gtcgacttgc atttattttt
      361 gcaaattttc ctttattttt tcaataatgt cccgaaggct gaaatccgat ttgaccataa
      421 atgttttcgc gcccagatcg agagctcttt gaacattgtc taattcttca agattagtca
      481 gaatgagcac cgggatgttt ttgtccgacc ccttttttat ttccgccagc acctcatagc
      541 cgtccttttt cggcaagatt aggtcaagta agatcaggtt gggtttttcc acttccgctt
      601 tttccatacc ttcttgtcca tcataagcgc ttattgtttc gtatccctcc tgggacaaca
      661 tttccaccag agcggactga agcgcccgat catcttcaat gatgagtatt ttttggggag
      721 tatccatatt gataaattta ttatttagta tttcgtattt tcaatgcaag acatttttca
      781 acaaagacgt gatgctagct ttgcgctggc aaactgaaat agaaaattga accttttccc
      841 gcttcgcttt caaaccaaac tttaccaccg caatgcccaa gaatagcctt cacgaggtaa
      901 agcccgattc ccaccccgtc agtgcggtag cgggattcat tctttgaacg gaaaaacttc
      961 tcaaaaatct tgctgtgctg atagcgcgga attcccactc cccaatcctg tacggagaaa
     1021 atgattttct ctttattttt cttcaactta atttcgactt ttcccccttc ttttgagtat
     1081 ttgatcgcat tcccgatgag attgtcaaag actactctca tctttttttc atcactacaa
     1141 actctctgcg gtccctctgc gatttgtgat tcaattttga cgttcttttc aatcgccatt
     1201 ccttgaaaac cgacaattga ttcttttgca agatccgcca aatcgaagat actttcctta
     1261 aaaaccgact ttccttggtc aacgcgggcc acctcaagaa gatccgtcac cattttgagc
     1321 atctgctcgt ttgatttctt gatattttga agaaaaacca tctgtttttc cggaaggcat
     1381 tcttttgtgt ccctttctgt ttcaatcacc tccatattcc acttaatggc ggaaagcgga
     1441 gtgcaaagct gatgagagat gatggaaatg aattcggttt tcatgatatt cgcttccgcc
     1501 actttttcta ccccccgaat gacggagctg ccaataatca taaggaaaac cacctcaatc
     1561 gtcacgctca tcaccgtcgt cccttcggaa gcgaaacggc tggaaacaaa aaatgtaccg
     1621 accatggcca cgatcgtgat gagccccatc atcaggaaca aaaaattcgg gcactgccaa
     1681 agtcccagcc cgtatttctt gcacgtcgtg ataaacctga aatcgtcgag aaattttgca
     1741 aacatttttg tttttattta aagaaatcag ccccctgttg ctttcaatta taccacaaat
     1801 aaagaaaaga aaccagtcac tggacgaaaa aagcctagct tttttcagct aagctttttc
     1861 gcagaaatat cgaacatttt acatcatgcc catgccgccc ataccgctca tgtccggcat
     1921 gccgccgccc ttttccttct tctctggaat atccgtgacg accgcttcgg tcgtgagaag
     1981 catcgcggcc gccgagacgg cgtttccgag cgcactgatc acgactttcg cggggtcgat
     2041 gatgccggcc ttgagcatat cgacatatgt gttttcgtcc gcgttgtatc ccattgtcca
     2101 atcggctttc gggttcactt cattgacgtg atattccatt tcaatttccg tcccggtttc
     2161 gatgttcact ttcttgcctt tggcggtctt tttgagaaaa gtatcctgca gatcctgaac
     2221 tctcgaaaca acaacttccg ccgattgtcc cgcattcgcg acaatctgtt tgatcggagc
     2281 gctcaaggca tcgacaagta tttgcgttgc aattcgatct tcatgagata aatttttctg
     2341 caaatcagct gaaagtttca ccgactcatt gagaagcgcg atcccgccac ccgccacgat
     2401 tccttgttca agcgccgctt tggtcgcctc aaccgcgtct tccacccgat gctgcttttc
     2461 tttctgctca acttcggtcg ccgccccgac cttgatgaca gccacgccgc cggacaattt
     2521 tccaagcctt tcctgcaatt tttctttgtc aaaatcactg gtcgcttttt cgatttgcgt
     2581 tttgagttgg gcgacccgcg cgtcaatatc ttttttcttg ccttttccgt cgacaagagt
     2641 cgtatcatct tttgtcgaga cgatccgtcc cgcttttcca agatcttcaa gagtcacgct
     2701 ctcgagtttc attccgcgtt cttcggaaat gacttgcccg ccggtcaaaa ttgcgatatc
     2761 ttcgagcatt gccttgcggc tgtcaccgaa tccgggcgct ttgatcgcca aaacattgag
     2821 tatgccccgc aatttgttca cgacaagcgt tgtcaaagcc tctccctcca cttcttcggc
     2881 gataataacc atctctttct ttccggtctg cgcaattttt tccaaaatcg gcaaaatttc
     2941 ctggacggaa gatatctttt tgtccgtgat caaaatttgg ggatctttaa gttccgcttc
     3001 catcgattcg gcgctcgtga tcatatacgg ggaaatatat cccttgtcaa atttcattcc
     3061 ctcgacgatt tcttcggcaa gaccaaaggt ttgcgactct tccaccgtga tcacgccttc
     3121 ttttccgact ttttcgatca ctttggcaat catttttccg acttccggat cctgcgcgga
     3181 aatggtcgcc acttgctcga tcgcgtcgcc ggaaaccacc ttagctgaag atatgagatc
     3241 ttttgaaacg cgattggaaa gtttcagtaa agcatttttg agctcaatta cattgatacc
     3301 tttcgaaata aatttcgcgc cggactcgac gatcgattgg gtgagaattg tcgccgtggt
     3361 tgttccgtca cccgccgcgt cgtttgtctt gttcgctact tccttcacga attccgcgcc
     3421 gatattttcg aatttatctt caagatcaat ttctttgacg atcgtcacgc cgtcattcgt
     3481 caccatcgga gaaccgaacc cacgatcaag cacaacattg cggccctttg gcccaagtgt
     3541 cacgcgaacc gcatccgcga ccttgtcgat cccggctttg attcttctgc gcgcatcttc
     3601 atcgaatcgt atttgctttg ccatatgttt tatttagagt taattattat tttgtcatcc
     3661 ctccacttcg cttcgctacg gtcggaatga cagacgagaa acccaattag cactctatgc
     3721 cttcgactgc caattgttat tatataaaat tttttttatt gtcaagggtt cactctcctt
     3781 tgccactcct cttcgacccg cacgccttgc ggttcgggaa ggaatatcaa atttttgtca
     3841 gtgtattcga tgtatgcgcc ttcttttttt gtcagcgggt tccgctcaat ccgcgtaatt
     3901 tgatggcgct tcagccagga ttcaattcta tcaccccaaa agagatttat caagctttca
     3961 aaagacctct gcactgccca ttgtagacgt cggacgtcta caaaatatct cgccggtttc
     4021 agctcgggaa tttcccaatt cggcttgatt tttttgatcc attccaaatt tatcccgcag
     4081 aatttctgaa aaatattata tccaaatatg ggatatataa aagaatacct tccc
//
LOCUS       DATKGS010000140         6601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_140506, whole genome shotgun sequence.
ACCESSION   DATKGS010000140 DATKGS010000000
VERSION     DATKGS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140506"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1227)
                     /locus_tag="VMQ48_03500"
     CDS             complement(172..1227)
                     /locus_tag="VMQ48_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HUD09127.1"
                     /translation="MGIFSRSKSAGGSNRKKRFYQKIWFKTLLVILILGAATGGWAMY
                     KTGTTLSKISTKDGFFSSLWKSIPGVPSGQLQGQNQGQINVLLLGMRGSNMPGGGLLA
                     DSIMVVAIRPQENKVALISVPRDLYVQDPGSSSQSKINAINALYEEKDDGKGMEMMKQ
                     VVSDVTGLQIHYVVRIDFKGFTELVDKLGGVDVYLDQPFSEPKQFEGEEAANFSLPAG
                     KNHLDGSKALFFTRARYASSDFERAKRQQQVLIALKDKMLSVGTLSDFGKLNNIMNIL
                     GDDVRADMDASEMRKFFDLANNLHDPQIKQKVFDTSPEGLLNASKADTPDGKTYILLP
                     AGGNYDKIREACRNIFQ"
     gene            complement(1313..2443)
                     /locus_tag="VMQ48_03505"
     CDS             complement(1313..2443)
                     /locus_tag="VMQ48_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUD09128.1"
                     /translation="MKIGIDIRLIGKKRTGDEVVFFNLVKNLTEIDRSNQYFLFTDRN
                     PEKNRELKAEIDKLKLADNFKIIFIDSPNRFWWNLWALPHFLNKNPVDVFHTQYIAPF
                     WLPKKVKLVLTIHDISFNFFPQFIKKSDLFFLKTLIPRSLKMASKIIAVSEFTKKEIE
                     KYYHIPAEKIAAIHNGVDFESFNQTISTEKLEEVKRKYNLPENFILYVGTFQPRKNIP
                     VVIEALKDLDIKLVLAGNRKAHNFDKRIDETIGKNNLGDRVIFPGWIDEEDKPALYKL
                     ARCFVFPSLYEGFGIPIIEAMAAGTPVVSSDIPCLTEVGGEGALFADPKKPQEFAKKI
                     REILSDKNLRDSLIKKGVEIAKSYTWQKNAEKTLGVYSGLAN"
     gene            complement(2526..3920)
                     /locus_tag="VMQ48_03510"
     CDS             complement(2526..3920)
                     /locus_tag="VMQ48_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUD09129.1"
                     /translation="MGALAKKRVTSNEKPNAEAVVIDTNVAVHDPESIDVLRDGGSRT
                     LCIPLVVIHELDGLKDKPDIGIDARDALRRIEDLTLGGEPSLKIISNLTFQGLNGLDR
                     KRADHQIIAAANTVIKNMKAKFASVILVSRDRPVRILAREFGFTAEDYTRDATEISDA
                     STMLPTFNIRSRNISSGFSFPYQPPEDGIDLPQNGGVICFSDWSGDPGTFGASDQKWG
                     AAFTAIRKDNKYKIIPNNIQLMGLKPYSLNGNGPNWHQHIAFSQLLDPNISLIFLQGG
                     AGSGKTLLAMAAAIELRSHYKKIFITRPMIHLEDQDKMGFLPGGIEEKTGPWMRPISE
                     ALDFLKEHAGATNRKTIEGMTEKKKFITEPLDYIRGMSYYRCILIIDEAQNLTPHQVK
                     TIITRAGEGTKIIFTGDLGQIDLRRRLDRRSSGLAYAISRMVGHEEECRMVAATNFKE
                     TVRSPLAKFGERVL"
     gene            complement(4023..5636)
                     /locus_tag="VMQ48_03515"
     CDS             complement(4023..5636)
                     /locus_tag="VMQ48_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUD09130.1"
                     /translation="MANFENFFENLKNILKKELSVLWIFSVLLGLALIVLQNKSKLPL
                     RPGDFVFISILALCAALYRPRWMFLLFVSLVPLENVIFASGFLPIQLRPYQFAGAILA
                     AAVIILYLAKRLNFKLLKPSWIDWLVIALAPLSFLSLLNSPNKNISLKNDLILFSFVV
                     LYYLVRNFLCDKKDFIEAAYFFIGSNLVVLFYGFWQVFADKFGGRSFEVMFGRPNSTF
                     TEADWLGIFLCFALAVLFSMTLSKAKTKIAKYALYIVIFLNLTLLILTLSRSAWIGAA
                     IVIFFWLVFSIYRKSEIRTSFTPRKFVNNLMLIILISLASLAAIQLGKLSKFDIFDRA
                     RSTATNEQRITIVCNNSANIPQTIANTDELAQYGCKFINLEEIGSYKSQGKIVTEIFR
                     KDPNVMTRSQIYRKSFEIIREHPILGVGFGTITQSLGSDERGTGLNESNIFLQIWASS
                     GILGLIAVVVIFGYLFIYSFRRISPVCPLNRIIGCPIVKDDFEKNLNIFLILGILALL
                     IPNLFNAGLLMGIFWLGLAIFASVKYLRD"
     gene            complement(5629..5952)
                     /locus_tag="VMQ48_03520"
     CDS             complement(5629..5952)
                     /locus_tag="VMQ48_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HUD09131.1"
                     /translation="MDLKQIIQRAAEVKEKYHELEMKKYGKRWTNSQIAEGFVGDVGD
                     LMKIIMAKEGLREMDNIDEKLEHELADCLYCVALLANKYGIDLEKAFLENMDKLETKI
                     SKNNG"
     gene            complement(5954..6283)
                     /locus_tag="VMQ48_03525"
     CDS             complement(5954..6283)
                     /locus_tag="VMQ48_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HUD09132.1"
                     /translation="MEKITYDKLIRDKIPEIIQKDGKESATHIAEDEEYVQKLKEKLE
                     EEVDELLSSDESHEIEEIADILEVLYAICNAKGISKDQIEEIRTQKAKERGGFEKRII
                     LEETRSK"
     gene            complement(6293..>6601)
                     /locus_tag="VMQ48_03530"
     CDS             complement(6293..>6601)
                     /locus_tag="VMQ48_03530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD09133.1"
                     /translation="KDLPALYRNCELFVLPSLYEGFGLMPLEAMASGAPTAVSKTSSL
                     PEIGGDAVLYFNPHDTDEISRVMGKVLRNNKLRLRLSEKGKERAKKFSWKSFVEKFFD
                     "
BASE COUNT         1780 a         1480 c         1354 g         1987 t
ORIGIN      
        1 cttcgcgcat cattatttcc aaatcttcct ctgtggagct gtcgaggacc aaaatttcaa
       61 aattttcaca aagaacattt tttttgagcg attcgaggca aagccgaagg acggctggat
      121 ttttgtagct ggttattatt actgataact caggatttcc gttgttcatt attattgaaa
      181 aatatttcgg caggcttcgc ggatcttgtc atagtttccg ccggctggca gcaaaatgta
      241 agtttttccg tccggtgtat cggcttttga ggcgttgagc agaccctcgg gggacgtatc
      301 gaaaactttc tgttttatct gaggatcatg caagttgttg gccaaatcga aaaattttct
      361 catttcagac gcatccatat ccgcccggac gtcatctccc aaaatattca taatattgtt
      421 caattttcca aaatcggaaa gtgttccaac gctcagcatt ttgtctttga gagcgatcaa
      481 aacttgttgt tggcgcttgg ctctttcaaa atcggacgag gcatagcgtg cccgggtgaa
      541 gaaaagcgct tttgatccgt caagatgatt ttttccggcc ggaagggaaa aatttgcagc
      601 ttcctcgcct tcaaattgct tcggttcgga aaaaggttgg tcgagataga catctacgcc
      661 tcccagcttg tccacaagct cggtgaaacc cttgaaatca attctcacga cgtagtgtat
      721 ttgaagaccc gtcacatcgc tcacaacttg cttcatcatt tccatacctt ttccgtcatc
      781 tttctcttcg tataatgcat tgattgcgtt tattttgctc tggctggacg atcccgggtc
      841 ctggacatag agatctcgag gaaccgaaat gagagccacc ttgttttcct gcggccggat
      901 agctaccacc atgatcgagt cggccaaaag tcccccgccc ggcatattgc ttccgcgcat
      961 accgaggaga aggacgttta tctgtccctg attttgtccc tgcagctggc ctgatgggac
     1021 gccggggatg ctcttccaaa ggcttgaaaa aaatccgtct ttcgttgaaa ttttgctcaa
     1081 agttgttccg gtcttataca tcgcccaccc accggttgca gctccaagaa tcaagataac
     1141 aagcaaagtc ttaaaccaaa ttttctgata gaatcttttt ttgcggtttg aaccgcccgc
     1201 tgattttgat cttgaaaaaa tccccataaa tattgaatac aaataaaaag aggcggattg
     1261 aaatcctcaa tgagtatagt ataccatttt ctgattggca atacaaagag attcagttcg
     1321 caagcccgct gtagactccc aatgtttttt ccgcgttttt ttgccaagta taacttttgg
     1381 cgatttcgac cccttttttg atcaaactat ctctcaaatt tttgtctgac aatatttctc
     1441 ggattttctt cgcgaattct tgaggttttt ttggatcagc gaaaagcgct ccctcgccgc
     1501 ccacttcggt gaggcatgga atatcggaag aaacaacagg cgttccggcg gccatcgctt
     1561 caatgatcgg gatcccgaag ccttcgtata gcgatggaaa aacaaaacat cgggccagtt
     1621 tgtataaagc cggtttgtct tcttcgtcga tccagccggg gaaaataact ctgtctccaa
     1681 gattattttt cccaatagtc tcgtcgattc ttttgtcaaa attatgcgct tttctgtttc
     1741 cggcgagaac caattttata tccaagtctt tcaacgcttc aatgacgact ggaatatttt
     1801 ttctcggctg gaatgttccg acgtaaagaa tgaaattttc aggcagatta tatttccttt
     1861 ttacttcttc taatttttcc gtagaaatgg tttgattgaa cgattcaaaa tcaacgccat
     1921 tgtggatcgc agcgattttt tcggccggaa tatgataata tttttcaatt tccttttttg
     1981 taaactccga aacggcgata attttggagg ccattttgag gctgcgcggg atcagagtct
     2041 tcagaaaaaa caaatctgat tttttgatga attgcgggaa aaaattgaaa gaaatatcat
     2101 gaatggtcag aaccaatttc accttttttg gcaaccaaaa cggcgcgata tactgggtat
     2161 gaaaaacatc cactggattt ttgttgagaa aatgcggcag agcccacaaa ttccaccaaa
     2221 aacgattggg agaatcgatg aaaataattt tgaaattgtc ggctagtttc aatttatcaa
     2281 tttcggcttt taattctcga tttttttccg gattgcggtc agtaaaaaga aaatattgat
     2341 tgctgcgatc gatctctgtc agatttttca ccaaattgaa aaataccact tcatcgccgg
     2401 ttcttttctt gccgatcaat ctgatgtcaa tgccgatttt catgtgttta tcatatccca
     2461 aatcctattt tttctcaaat aaataagccc caggcggaaa gcccagggct tggaagtggg
     2521 tgagatcaaa ggactctctc gccgaatttg gcaaggggtg atcttacggt ttccttgaag
     2581 ttggtggcag ccaccattcg gcactcctct tcatgaccaa ccatgcggct tatggcataa
     2641 gcgagaccgc ttgatcggcg atccagccgg cggcgaaggt caatttggcc gagatcgccg
     2701 gtaaaaatta tctttgtgcc ttcgcctgcg cgggtgatga tggttttgac ctgatgaggt
     2761 gtcaggttct gcgcctcatc aataatcagt atacaacggt aataggacat accccggatg
     2821 tagtccaagg gctcggttat gaacttcttt ttctcggtca tgccttcaat ggtcttgcga
     2881 tttgtggctc ccgcatgttc cttgagaaaa tcaagcgcct ctgatatcgg gcgcatccaa
     2941 gggccagtct tttcttcaat gccgccggga agaaagccca tcttatcctg atcctcaaga
     3001 tgaatcatcg gacgggtaat aaagattttc ttgtagtgag accgcaattc aatcgcagcc
     3061 gccatcgcca gcaacgtttt gcccgatccg gcaccgccct ggaggaaaat caaggaaata
     3121 ttgggatcga gcaattggga aaaagcgatg tgctgatgcc agtttgggcc gtttccattc
     3181 aatgaatagg gctttaaccc cattagctga atgttattcg ggataatttt atacttatta
     3241 tctttccgaa tagcagtaaa cgctgctccc catttttggt cgctggcacc gaatgttccg
     3301 ggatctccag accaatcact gaagcagatt acgcctccgt tttgaggcag gtcaatgccg
     3361 tcttcagggg gttgatatgg gaaagagaat ccagacgaaa tattccgcga tcgaatattg
     3421 aatgtcggaa gcatagtcga agcgtcggat atctcggtcg catcgcgggt ataatcctcc
     3481 gccgtgaaac cgaattctcg cgccaaaatc cttaccggac gatcgcgaga gaccagaatc
     3541 acgctggcaa atttagcctt catatttttg atcacggtgt ttgctgctgc gataatttgg
     3601 tgatcggcgc gctttctatc gaggccattg agtccttgaa aagttaagtt actgatgatc
     3661 ttcagcgaag gctcgccacc gagcgttaaa tcctcaatgc gtctcaaggc gtctcgagca
     3721 tcgatcccaa tatccggttt atctttcaaa ccatccagct catggatgac gactaagggg
     3781 atacagagtg tgcggctacc gccatcccgc aacacgtcga ttgattccgg gtcgtgaact
     3841 gcgacattgg tgtcgatcac aacggcttca gcgtttggtt tttcattgga cgttacccgt
     3901 tttttggcca aggctcccat aaaagtctcc tcccccatta tagggataaa tagatatggt
     3961 tcattcgcct aatttttaaa gatccaattc ttctttctga gtccactata aatctatttt
     4021 ctttaatcgc gcaagtattt aacgcttgcg aaaattgcca gtcccagcca gaaaattccc
     4081 atcagaagtc ccgcattgaa taaatttggg attagcagtg ctaagattcc taaaattaaa
     4141 aatatattca gatttttttc gaagtcatcc ttcacgatcg ggcagccgat tattctattg
     4201 agtgggcaaa ccggtgagat ccttcggaaa gaataaataa ataaatatcc aaaaataacg
     4261 acaacggcaa tcagtcccaa aatgccactg ctcgcccaaa tttggaggaa aatattgctc
     4321 tcattgagcc ctgttccccg ttcgtcggat ccgagcgatt gcgtaatcgt cccaaaacca
     4381 acgccaagaa ttggatgctc gcggatgatc tcaaaacttt ttcgataaat ttggctgcgg
     4441 gtcatcacat tcggatcttt tcggaagatc tcggtgacga tttttccttg cgatttatac
     4501 gaaccaattt cttcaagatt aatgaacttg cagccgtatt gtgccaattc gtcggtgttg
     4561 gcgattgttt gcggaatatt cgcactatta ttgcagacaa ttgttattct ctgttcattg
     4621 gttgcagttg accgggcgcg gtcaaagatg tcgaatttgg aaagttttcc aagctgaatc
     4681 gcagccaggg aagccaaaga aatcaaaata atcaacatta aattattgac gaattttcgg
     4741 ggcgtgaagc ttgtcctaat ttcgcttttt ctatatatgc tgaaaaccag ccagaagaaa
     4801 atcacaattg ccgctccgat ccatgccgaa cgggaaagtg tgaggatcaa taaagttaaa
     4861 ttaagaaata tcactatgta taaggcatat ttggctattt tggtctttgc ttttgataat
     4921 gtcattgaaa aaagaacggc cagcgcaaaa cacaaaaata ttcccagcca atccgcttcc
     4981 gtgaatgtgg aatttggtcg gccaaacatc acttcaaacg atcttccgcc aaatttgtcg
     5041 gcgaagactt gccaaaatcc atagaaaagc acaaccaaat ttgagccaat gaaaaaatac
     5101 gcggcctcaa tgaaatcttt cttgtcgcac aagaaatttc gaacaagata ataaagaacg
     5161 acaaaagaaa agaggattag atcgtttttc aagctgatgt ttttattcgg tgaattcagg
     5221 agtgatagaa agctcaatgg cgcgagcgca ataaccagcc aatcaatcca ggaaggtttt
     5281 aataatttaa aattcaatct ttttgctagg tataaaatga ttactgcggc ggctaaaatc
     5341 gcaccggcaa attgatacgg ccgcaattgg atcggcaaaa atccggaagc gaaaatcacg
     5401 ttttcgagcg gcacgagtga aacgaaaagc aaaaacatcc atctcggacg atacagcgcg
     5461 gcgcaaagtg cgagaattga aatgaaaacg aaatctcccg gccgaagcgg cagcttgctc
     5521 ttgttttgca aaacaatcaa cgccaatccc agcagcacgc tgaaaatcca aagaacagaa
     5581 agttcttttt tgagaatatt tttcaaattt tcgaaaaagt tttcaaaatt agccattgtt
     5641 ttttgaaatt tttgtttcca gcttatccat attttccaga aaggcttttt ccagatcaat
     5701 tccgtactta tttgccagta aagcgacaca atacaagcaa tcggccagct catgttccag
     5761 cttttcgtcg atattatcca tttctcgaag tccttccttg gccataataa ttttcatcaa
     5821 atcaccgacg tcacccacaa aaccctcggc gatctggctg tttgtccatc tttttccata
     5881 ttttttcatc tccaattcgt gatatttttc tttcacttcc gccgctcttt gtatgatttg
     5941 cttaagatcc atattatttg cttctagttt cttcaagaat tattcttttt tcgaatccac
     6001 ctcgttcttt ggctttttgt gttcttattt cttcgatttg atctttactt ataccctttg
     6061 cattgcaaat tgcgtaaaga acttccaata tatccgcgat ttcttcgatt tcatgacttt
     6121 catcgctact taaaagttca tcaacttctt cttcaagttt ctctttaagc ttttgtacat
     6181 attcttcatc ttcagcaata tgagtagctg attctttgcc atctttctgg ataatttcag
     6241 gtattttatc ccgtataagt ttgtcgtatg ttattttttc cataaatata aattagtcaa
     6301 aaaatttctc cacaaaactt ttccagctga attttttggc gcgttctttc cctttttcgc
     6361 ttaatcgaag tctcaattta ttgtttcgca aaactttccc catcaccctg gatatttcgt
     6421 ccgtgtcgtg cggattgaaa tacagaaccg catcgccgcc gatttcgggg agcgaggaag
     6481 tttttgaaac agcggttggc gcgccactgg ccatcgcttc aaggggcatc aggccaaatc
     6541 cttcatatag cgacggcaag acaaaaagtt cgcaattccg atacaaggcc ggcaggtctt
     6601 t
//
LOCUS       DATKGS010000141         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_69084, whole genome shotgun sequence.
ACCESSION   DATKGS010000141 DATKGS010000000
VERSION     DATKGS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69084"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..2149)
                     /gene="uvrB"
                     /locus_tag="VMQ48_03535"
     CDS             complement(191..2149)
                     /gene="uvrB"
                     /locus_tag="VMQ48_03535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HUD09134.1"
                     /translation="MKFKISSKFKPDGDQPKAIKKLTAGLRRGDKFQTLLGVTGSGKT
                     FTMANVIENVQKPTLVIAPNKTLAAQLAQEFRTFFPKSAVEYFVSYYDYYQPEAYIAS
                     SDTYIEKESQINDEIDRLRHAATVAVMSRKDVVIVASVSCIYGLGSPEFYEEISLSLK
                     VGEAADREKLIRDLTEMHFIRSEYLSRGRFRSHGDTVEVMSPSREIVTRIEIKGDKIG
                     KISEYDFLTGEVLRKLEKVLIYPAKHFVVPEPVLEEALKQIEKELNERVAYFKKHKKL
                     LEAERIYRRTKQDLEMMREIGYCNGIENYSRYLTGRAPGEAPYTLVDYFNKNFLIVID
                     ESHVTVPQLNGMYNGDHARKKALVDNGFRLSSAYDNRPMKFSEFEKKINQVIFTSATP
                     ADYEKKNSAQIVEQIIRPTGLIDPELVIKPAKGQVKDVIEEIKSVVAKKERVLVTTLT
                     KKMAEDLAEFLKENEIKSEYLHSGVDTMDRVRILESLRRGKFDVLVGVNLLREGLDLP
                     EVSLVTILDADKEGFLRSETSLVQTIGRAARNISGRVILYADKVTGSMKRAIDETNRR
                     RETQTKYNKAHHITPKTIQKKIESIVDHELKPEVPREFVKLESLEDIAGYIKQKEAEM
                     KIAARNMEFEKAAVIRDEIIQLRKLQMG"
     gene            2231..>2503
                     /locus_tag="VMQ48_03540"
     CDS             2231..>2503
                     /locus_tag="VMQ48_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HUD09135.1"
                     /translation="MFKRGIATFTLDYGKCPKWLFERMVKLGREMARVIIAEYGPDEF
                     VKRIADPVWFQALGTVLAFDWNASGLTTILTAALKEAIRGEERDLGI"
BASE COUNT          596 a          604 c          539 g          764 t
ORIGIN      
        1 gatccactga ttcccgtgaa cacgataaac ttgcctcggg gcagttcgag gtttatgttc
       61 ttcaaattat gctcgcgagc accgcgaatt attattttat ccatatattc ttatttccta
      121 acacaaaaat gccccattta agggtttcat ctatagtaat actattgagt taataaatca
      181 atttcaactc ttatcccatc tgcaatttcc ggagctgaat tatttcatcc cggatcaccg
      241 ctgctttttc gaattccata tttcgggcgg cgattttcat ttcggcttct ttttgtttga
      301 tatatccggc gatatcttca agggattcca gtttcacgaa ttcgcgcgga acttccggct
      361 ttagctcgtg atcaacgatc gattctattt ttttctggat ggttttcggg gtgatgtgat
      421 gtgccttgtt gtatttcgtc tgcgtttcgc gtcggcgatt ggtttcgtca atcgcccgtt
      481 tcatcgagcc ggtgactttg tcagcataga gaatcacccg cccggaaatg tttctcgctg
      541 ccctcccaat cgtctgcacg agtgaagttt ccgagcgcaa aaatccttct ttgtccgcat
      601 cgagaattgt cacaagcgac acttccggca aatcgaggcc ctcccgaagc aagttcactc
      661 ccactaaaac atcgaacttg cctcttcgca gactttccag tatccgcacc cgatccatcg
      721 tgtcgactcc cgaatgcaga tattccgatt ttatttcgtt ttctttcaaa aattccgcca
      781 aatcttccgc catttttttt gtgagcgtcg tcacgagcac ccgttctttt ttcgcgacta
      841 cgctttttat ttcttcaatc acgtccttca cttggccttt ggctggttta atgaccagtt
      901 ccggatcgat gagacctgtc ggacgaataa tctgttcgac aatttgcgct gaatttttct
      961 tctcatagtc cgctggagta gccgaagtga aaataacttg atttattttc ttttcaaatt
     1021 cggaaaattt catcggccga ttgtcataag cgctcgacag ccgaaaaccg ttatcaacaa
     1081 gagctttttt gcgcgcgtgg tcgccgttat acattccgtt gagttgggga actgtcacat
     1141 gcgattcgtc aatgacaata aggaaatttt tattaaaata gtccacgagt gtatatggcg
     1201 cttcgcccgg cgcgcgaccg gtcagatagc gggaataatt ttcaattccg ttgcaatacc
     1261 cgatttcccg catcatttcc aaatcctgtt ttgtccggcg atagattctt tcggcttcga
     1321 gcaatttttt atgttttttg aaatatgcga cccgttcatt gagttccttt tctatttgct
     1381 tcaaagcctc ttccagaacc ggttcgggca caacaaagtg cttggccgga tagatcaaaa
     1441 ctttttccag ttttctcagc acttcgccgg tcaaaaaatc gtattcgctg atcttgccta
     1501 ttttatcccc ttttatttca atgcgagtca caatttcccg cgaaggcgac atgacttcaa
     1561 ccgtgtctcc atgagaacga aatctcccac ggcttaaata ttccgagcgg ataaaatgca
     1621 tttctgtgag atctcggatt agtttttctc ggtccgccgc ttcgccaact ttgaggctca
     1681 aagaaatttc ttcgtagaat tcgggcgatc ccaagccgta gatgcatgat accgaagcga
     1741 caataaccac gtctttgcgc gacatcaccg ccaccgtcgc cgcatgacgc agacggtcaa
     1801 tctcgtcatt tatttgggat tctttttcaa tgtaagtgtc agatgacgca atataagctt
     1861 ccggctggta atagtcatag taggaaacaa aatattccac cgcgcttttt gggaaaaatg
     1921 tccggaattc ctgcgcaagt tgcgcggcga gcgttttgtt cggcgcgatg accagcgtcg
     1981 gcttttgcac gttctcgatc acattcgcca tcgtgaaagt ctttcccgat cccgtcacac
     2041 caagaagggt ttgaaatttg tccccacgcc gaagcccggc ggtcaatttc ttgatcgcct
     2101 tgggttgatc cccatcgggt ttgaatttgg atgatatttt aaatttcatg tccaatcatt
     2161 atatacgcaa gaaaagaaaa agtccatata tagactagca taattcatag aatagtgata
     2221 tagtgatagt atgttcaaac ggggaatcgc cacattcacg ctcgactacg gcaagtgtcc
     2281 gaaatggctt tttgagcgga tggtgaaatt gggacgggag atggcccgag tcatcattgc
     2341 cgaatatggg ccggatgagt ttgtgaaacg gattgccgat ccggtctggt ttcaggcgct
     2401 gggaacagtg ctcgctttcg attggaacgc atcgggactg acgacaatat taacagccgc
     2461 tctcaaagaa gccattcgcg gtgaagaaag ggatttggga ata
//
LOCUS       DATKGS010000142         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_24750, whole genome shotgun sequence.
ACCESSION   DATKGS010000142 DATKGS010000000
VERSION     DATKGS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24750"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="gltX"
                     /locus_tag="VMQ48_03545"
     CDS             <1..951
                     /gene="gltX"
                     /locus_tag="VMQ48_03545"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HUD09136.1"
                     /translation="GDVVRGKTKFVNKNLDDFVLLKSDGYPTYHLAHIIDDHLMKTTL
                     VIRGEEWLPSAPKHILLFQAFGWEPPQYAHLSTILNKNTKKKLSKRDGDVSVEDFKDK
                     GYLPEAVVNFIALLGWNPKTEKEIFTLSELAEEFDVAKLNKAGAIFDLEKLDWMNGLY
                     IREKNIKELVELCRPYLAKYEEKLSGDYLEKIVSVERERMKKISDITENTEFYFDAPE
                     YDGKILKWKDMRDDDLKNSLNKSLEILSEIEEKNWELENLKNRLMEAADPGSRGELLW
                     PLRVALTGKEKSPSPFEVAWVIGKDESIKRVSNAIELAKG"
     gene            1058..2341
                     /locus_tag="VMQ48_03550"
     CDS             1058..2341
                     /locus_tag="VMQ48_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HUD09137.1"
                     /translation="MITKHTFAQSATNGTSSSGATNPATTSPADQQQIQDKQQQIQDL
                     EQKAESYRQMIDLNQSKAQTLQNQIKLMESQISSLESDVASLQQKIDSTDQDIGTLST
                     QIETKQKDIQAQKVVLGQLLQAYYENDQDTLTEMLLKNSSFSEFMNQSEYISQTGSRV
                     DSVLASISDAESQLENAKKTLEAKNEQQKEQRGNISQQKLALDGEQASKQQLLADTNG
                     QEQKYQDLLQHVEEEKQSLLGDIDELSQQKSSDLAAVEASIQKPKSGIASTSWYFNQR
                     DSRWGDQGIGYSNTLMKSYGCAVTALAMVLDYHGVNIDPGTLAKQPIFSHDLIVWPQS
                     WKGVNLVSSTGHGNIDWNVIDTELKNKNPVIVFVKATKRGAGHYVVIHDKDKNGDYVV
                     HDPYWGANIFLSSTKAYVGALYGSGVVVDQMIIYH"
     gene            2354..2560
                     /locus_tag="VMQ48_03555"
     CDS             2354..2560
                     /locus_tag="VMQ48_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HUD09138.1"
                     /translation="MKRKIILASKSPRRKSLLEKIGLKFDTRESKYEEDMKKYSDPYK
                     LVKYLALKKAEQGVLTSIIAYNVA"
     gene            complement(2720..3160)
                     /locus_tag="VMQ48_03560"
     CDS             complement(2720..3160)
                     /locus_tag="VMQ48_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HUD09139.1"
                     /translation="MYSKKVLQYFMKPKNQGSMKNPDGFGTVGNPRCGDIMTVYIKVK
                     KLKNKEFISDIKFETLGCAAAIATSSMVTEMAKGKNLDDAFKITRQDVADELEGLPPL
                     KMHCSNLAEGALKRAIEDYRSKAGSKAKTPEKTKKASASKKKKK"
     gene            complement(3179..4375)
                     /locus_tag="VMQ48_03565"
     CDS             complement(3179..4375)
                     /locus_tag="VMQ48_03565"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HUD09140.1"
                     /translation="MAKRKIYLDYASTTPIDPRVFAVMKPYFSGKFGNPSSMHSFGQE
                     ASEAVEKARSQAADFFGCESGEIIFTSGATESNNLAIKGMIISKISHFSPKKQKPHVI
                     TTQFEHSCVLNSCKKLEEQGMAEVTYLPVGKDGLVSVGEVKKNIRPNTALITIMYANN
                     EIGTIQPIKEIGKLVAKENVGREEKILLHTDAVQAVNYLDCNVDNLGVDLLTASAHKI
                     YGPKGIGLLFIRKGTPLKRIQDGGGHEFNLRAGTLNVPGIVGFGTALEIAQQERARNY
                     KIVEKLRDHMFARVMKEIKGAKINGSKIKRIPNNANFIFRDVEGEGMLLGLDMEGVAV
                     STGSACSSGDLRPSHVLTAIGISEEESHGSLRVTLGKYTTKQEIDFFIDSLEEVVARL
                     RKIAGK"
     gene            complement(4387..>4621)
                     /locus_tag="VMQ48_03570"
     CDS             complement(4387..>4621)
                     /locus_tag="VMQ48_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09141.1"
                     /translation="KYGPLVMRFMHLSKMSPLGKYKAGDIIGCTGNTGKITKSPHLHI
                     DLSRNQLDLKNFPNFIDPEEFFSNRLARNSFSR"
BASE COUNT         1307 a          997 c         1085 g         1232 t
ORIGIN      
        1 ggagacgtag tgaggggaaa aactaaattt gtgaacaaaa atcttgatga ttttgttttg
       61 ctcaaatcag acggctatcc gacctatcat cttgcccata tcatcgatga tcatctgatg
      121 aaaacgactc tcgtgatcag aggagaggag tggctgccga gcgcgccgaa acatatcctg
      181 ctttttcagg ctttcgggtg ggagccgccg cagtatgccc acctttcgac aattctcaac
      241 aaaaacacga aaaagaaatt gtccaagcgc gacggggacg tgagcgttga agatttcaaa
      301 gacaaaggct atcttcccga agcggttgtg aatttcatcg ccctgcttgg ctggaacccc
      361 aaaacggaga aggaaatttt cacgctttcg gagctggcgg aagaatttga cgtggcgaag
      421 ctcaacaaag cgggagcgat ttttgacttg gaaaaattgg attggatgaa cggactctat
      481 atccgcgaaa aaaacatcaa agaattggtt gagctttgcc ggccgtatct cgcaaaatac
      541 gaagaaaaat tgtctggcga ttatctcgaa aaaatcgtga gcgtggaaag agagaggatg
      601 aaaaaaattt ccgacatcac tgaaaacacc gaattttatt ttgacgctcc cgagtatgac
      661 ggaaaaattt tgaaatggaa agatatgcgc gatgatgatt tgaaaaattc actgaataag
      721 tctctggaga tcctttccga aatagaagaa aaaaattggg agctggaaaa tctgaaaaac
      781 cgcttgatgg aggcggccga tccgggcagt aggggagagc tgctgtggcc tttgcgtgtc
      841 gctttgaccg gaaaagaaaa aagtccgtcg ccattcgagg tggcttgggt gataggcaag
      901 gatgaaagta ttaaaagagt tagtaatgcc atagagttgg caaaaggtta agctagtgaa
      961 tataaaaaat ttcaaatttc aaatttcaaa tttcaaattt aggtgtcgct acgcgacgac
     1021 agctttttat ttccttttta tattcctgac cgcgaatttg atcacaaaac atacttttgc
     1081 ccaaagcgct acaaatggca cgagcagtag tggtgcgacc aatcctgcga ccacaagtcc
     1141 ggctgaccag cagcaaatcc aggacaagca gcagcaaatc caggatcttg agcaaaaggc
     1201 ggaaagctat cgccagatga tcgatttgaa tcaaagcaag gctcagacgc tccaaaacca
     1261 gataaagctc atggaaagcc agatttcatc cctggagagc gatgtagcaa gcctacagca
     1321 gaaaattgac tctacggatc aggatattgg gacactttcc acacaaattg aaacaaagca
     1381 aaaagatatc caggcgcaga aagttgtttt gggccagttg ctccaggcct actatgaaaa
     1441 tgatcaggac acactcactg aaatgttgct caagaattcc agtttttccg agtttatgaa
     1501 ccagtcggaa tatatctcac agacaggttc cagggtggat agtgttctgg cttcgatcag
     1561 cgacgcggaa agccagctgg aaaatgcgaa aaaaacgctt gaagcgaaaa acgaacagca
     1621 aaaggagcaa agaggaaata tctctcagca aaaactggcg ctcgatggcg aacaagcctc
     1681 aaaacagcag ctcttggctg atacgaacgg tcaggagcaa aaatatcagg atcttctgca
     1741 acatgttgaa gaagaaaagc aatcactctt gggcgacatt gatgaattga gccagcaaaa
     1801 gagttccgac ctggctgcag ttgaagcaag catccaaaaa ccaaagtcag gtattgcttc
     1861 cacaagctgg tatttcaacc agcgtgattc gcgttgggga gaccaaggca ttgggtattc
     1921 aaatactctc atgaaaagct atggttgcgc ggtgacggcc ttggcgatgg ttctggacta
     1981 tcacggcgtt aatatcgatc cgggcacgct tgccaagcag ccgattttct cgcatgacct
     2041 gattgtctgg ccacaatcat ggaaaggtgt gaatcttgtc tcctcgaccg gacacggaaa
     2101 cattgattgg aatgtcattg acacggagct gaaaaacaaa aatccggtca tcgtttttgt
     2161 gaaagccaca aagaggggag cagggcacta tgtcgttatt catgataaag acaaaaacgg
     2221 cgattatgtc gttcatgatc cctattgggg agcgaatatt ttcttgagtt cgacaaaagc
     2281 gtatgtgggt gcgttgtatg gcagtggcgt cgtggttgac cagatgataa tttatcatta
     2341 aaaaaatatt tttatgaaaa gaaaaattat tttagcctca aaatcaccgc ggagaaaaag
     2401 tttattagag aaaattgggt tgaaatttga tacgagagaa agcaaatacg aggaagacat
     2461 gaagaaatat agtgacccat ataagttagt taaatattta gcgcttaaaa aagccgagca
     2521 aggagttcta acttctatta tagcatataa tgtcgcataa cctacattgt gcgacgttgt
     2581 atatacaccc tgaccccact tccctcccaa aatattggga aaattaaaca aaataaaaaa
     2641 tttatatttt caataaaaag cccccgctct attccgcggg ggcttgcttg tatatataga
     2701 cgtgtataca cggaaaaaat tatttctttt ttttcttgct cgcgctcgct tttttggtct
     2761 tctccggcgt cttcgccttc gatccggctt ttgacctata atcctcaatt gcccgcttca
     2821 gggcgccttc cgcaaggttt gaacagtgca tcttcaaggg cggaagacct tcaagctcgt
     2881 cagccacgtc ctggcgagtg attttgaagg cgtcgtccaa atttttcccc ttggccattt
     2941 ctgtcaccat cgatgatgtt gcgatggcgg cagcgcagcc cagcgtttca aatttgatgt
     3001 cagaaataaa ttctttgttt ttcagtttct tcaccttaat ataaactgtc atgatgtcgc
     3061 cgcatctggg gttgccgacc gtgccgaaac catccggatt tttcatcgat ccttggttct
     3121 tcggcttcat gaaatattgg agaacttttt tggaatacat atgatcgaat atgaaatatt
     3181 attttcccgc gatcttgcga agtcttgcaa caacctcttc gagggaatcg ataaaaaaat
     3241 caatttcttg tttggtcgtg tattttccca gtgtcacgcg aagactgccg tgactttctt
     3301 cttctgaaat tccaatggcg gtcaagacgt gcgacgggcg aaggtctccg gaagagcagg
     3361 cgctgccggt agaaacggcg acgccttcca tatccagccc cagaagcatc ccctcaccct
     3421 ccacgtcgcg gaaaataaag ttggcgttgt tgggaattct ttttattttc gatccattta
     3481 tttttgcgcc tttgatttcc ttcattacgc gggcaaacat atggtcgcgt aatttttcaa
     3541 caattttata attccgagct ctttcctgtt gggcgatttc aagcgcggtg ccaaagccga
     3601 caattcccgg aacgttgagt gttccggcgc gcaagttgaa ttcgtgccct ccgccgtcct
     3661 ggatcctctt gaggggcgtg ccttttcgga taaagagaag tccgatgcct ttcggtccat
     3721 atattttgtg cgctgaagca gtgagaagat cgactcccaa attgtccacg ttgcaatcaa
     3781 gatagttcac ggcctgcacg gcatcggtat ggagcaaaat tttttcttcg cggccaacat
     3841 tttctttcgc aaccaacttc ccgatttctt tgatgggttg aatggtgccg atctcattgt
     3901 tggcgtacat tatagttatg agagccgtat tcggcctgat attctttttc acctcgccaa
     3961 cgctcaccaa cccgtccttt ccaaccggaa gataggtcac ttctgccatc ccctgctctt
     4021 ccagtttctt gcacgaattc aaaacgcagg aatgttcaaa ttgagtggtg atgacgtgcg
     4081 gcttttgctt ttttgggcta aagtgggata tttttgagat tatcattcct ttgatcgcaa
     4141 gattgttgct ctcggtcgcg ccggaagtga aaatgatttc tccggattcg cacccaaaaa
     4201 aatcagccgc ttggctgcgc gctttttcca cggcctcgct cgcctcttgt ccaaaggagt
     4261 gcattgatga cggatttccg aattttccgg aaaaataggg cttcatcacc gcaaaaaccc
     4321 gcggatcaat gggggtggtg gacgcataat caagatatat ttttcgcttc gccatacaat
     4381 attatactat cgcgagaagg agtttcttgc aagacgattc gagaaaaatt cctccgggtc
     4441 gatgaaattg ggaaagtttt tcaaatccaa ttgattgcgg ctcaagtcga tgtggagatg
     4501 cgggcttttg gtgatttttc cagtgtttcc ggtgcagcct atgatatccc ctgctttgta
     4561 ttttccgagt ggcgacattt tggaaagatg catgaagcgc atgacgagcg gaccgtattt
     4621 t
//
LOCUS       DATKGS010000143         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_85002, whole genome shotgun sequence.
ACCESSION   DATKGS010000143 DATKGS010000000
VERSION     DATKGS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85002"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VMQ48_03575"
     CDS             complement(<1..755)
                     /locus_tag="VMQ48_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HUD09142.1"
                     /translation="MSKFLVKSFFWVTISEVIYNLSAYVIHAVMGRVLGPADYGRFGI
                     IVTLTTMTVVLIGQSVPTAMSKYLSEIFEKEKGLIPIIKWQTIKIQSVIIGSVTVLFF
                     LLSPVIARVLNDPTLTNLFRLSSLVIPAFALASFYFSYYTGIQKFKIQAFLKTNRGIM
                     RIIFIIGLAFLLKAHGMALQGAILGYVVAPLSIFFEAWSLDPYKKLKAAGTFDWKKLI
                     VFAWPVTIFLIAYQLLNTIDLYLVKGILRSDFQT"
     gene            complement(737..1324)
                     /locus_tag="VMQ48_03580"
     CDS             complement(737..1324)
                     /locus_tag="VMQ48_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09143.1"
                     /translation="MKLITISGLDGSGKSTQIKLLKDYLKSQDKTFFYFHAVEFSFGN
                     KVLGKKKDKTGKEVSVKNAGWLKIFLRKISLLIDICRFKRLVKKLKVDYIISDRYFYD
                     SIININYLSEKNSKLCAEKYIPKPDFAFYIDVKPEIIMQRERKPDQGMEYLLAKEKIF
                     SEKIKDWDIVSVDGEKNKDEIFSEIKSKIKCLNFS"
     gene            complement(1329..2777)
                     /locus_tag="VMQ48_03585"
     CDS             complement(1329..2777)
                     /locus_tag="VMQ48_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HUD09144.1"
                     /translation="MKIESLSTHKKKFQTDRMKVPAILHASDELLPNDKTLAQLENTA
                     SQEYVFHHIAALSDVHDKKGRKNPTGTVVASEKYLLPQINDTAPNCGMRFLKTNLTDA
                     DLTPENIDKLFNALLKPVPTKAYVGTPVPYSLVVDICRYGLKPVTEYFHTRTKNEIEN
                     TFKNGNFFDQEISQQDITDAIPQLFLWIGKFRLGILGAAGNHFLDLMKISEIKDPEMA
                     EKFGLKKGQYIFMFHTGSGLLGQYASYLYTPKKKEHLSQRIVLELGKLFWRSGKKNIY
                     KKIAKKIAEYKNKDEFFAYDADSPEGKMFLTAHQAAGNQGFANRSILTHNLDQTIEKI
                     LGKDPEFDLLYDSPHLFINRENHFGKDVWVHRNGAVRAQAGEPVFIPSSMSTPMYLGI
                     GTDENESTFFSAGHGTGRRKNPETNTAGTKEELFAKMNNKKVKLFNAKSKGVVFQDSG
                     YYKDVEEVIAGMEENKIVKPVAKMEPIAVLMY"
     gene            complement(2774..>3520)
                     /locus_tag="VMQ48_03590"
     CDS             complement(2774..>3520)
                     /locus_tag="VMQ48_03590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUD09145.1"
                     /translation="AEKLKKIIHADFIITYSDYAKEKLEKMGFNRIKRIYPGIDVNLY
                     SPAPKNQQLMKKFATSEKDFVINYTGEYVRLGDMDDIIESFSGLAKRYQNFKLHLAVR
                     VKNQKDAEKKREIIDKLKKEDVLGRVAFIDDGSYTMEEIYNLSDISIFPARTMAGKFD
                     IPLAVIEAMACGKPVIASSLERLKYFLNNENSILIPPGNREVLKQKILYLCDNPEVRN
                     DLARRGIDFVRDNFDITKIVKEYEEVYENS"
BASE COUNT          930 a          768 c          716 g         1106 t
ORIGIN      
        1 gtctgaaaat cggaacgcaa aattcctttc acgagataga ggtcgatggt gttgagaagt
       61 tgataggcga tgagaaaaat cgtcaccggc caggcaaaga cgattagctt tttccagtcg
      121 aatgttccag cagctttcaa ttttttgtac ggatcaagcg accaggcttc gaagaaaata
      181 gaaaggggag cgacgacata tcccaaaatt gctccctgga gcgccattcc atgcgccttg
      241 agcagaaacg ccagcccaat aatgaaaatg atccgcatta tgccccggtt cgttttgaga
      301 aaggcctgaa tcttgaattt ttggatgccg gtataatagg agaaatagaa cgaagccaga
      361 gcaaaggcgg gaatcacaag cgaggaaaga cggaacaaat tggtgagcgt cggatcgttc
      421 agcacgcggg cgatcaccgg cgagaggaga aaaaaaagca ccgtcactga tccgatgatc
      481 acgctttgga ttttgatcgt ttgccacttg atgatcggaa tcaacccttt ttctttctcg
      541 aatatctcgc tcaaatattt ggacattgcc gtcggcacgc tctggccgat gaggacgacg
      601 gtcatcgtcg tgagcgtgac gatgattcca aagcggccat aatccgccgg ccccaaaacc
      661 cgccccatca ccgcatgaat aacataagcc gagagattat aaataacctc gcttatcgtc
      721 acccaaaaaa atgattttac gagaaattta gacattttat ttttgatttt atttcagaaa
      781 atatttcgtc tttatttttt tcgccatcaa ctgaaactat atcccaatct tttatttttt
      841 cagaaaatat tttttctttt gcaagtagat attccattcc ctgatcaggc tttcgctcgc
      901 gctgcatgat gatttctggc ttcacatcga tataaaaagc aaaatctggt ttgggaatat
      961 atttttcagc gcacaatttg ctgttttttt cggaaagata atttatattt ataatagaat
     1021 cataaaaata ccgatcggaa attatataat caacctttaa tttcttcact aatcgcttaa
     1081 acctgcaaat gtcaattagg agactgattt tacggagaaa tattttgagc caacccgcat
     1141 ttttaacgct gacttctttg cctgttttat ctttcttttt tcccaaaact ttattcccaa
     1201 aagaaaactc caccgcgtga aaatagaaaa aagtcttatc ttgcgacttg agataatcct
     1261 tcaacaattt aatttgcgtt gatttcccgg acccatcgag gccggaaatg gtgataagtt
     1321 tcaatttttt aatacattaa aactgcaatg ggttccattt tcgccaccgg tttcacgatc
     1381 ttattttctt ccattcccgc gatgacctct tccacgtcct tataatatcc cgaatcctga
     1441 aaaaccacgc cttttgattt ggcgttgaac aatttgactt ttttattatt cattttcgca
     1501 aacaattctt cttttgtgcc ggctgtattt gtttcaggat tttttcgacg acccgtgcca
     1561 tggccggcag agaaaaatgt tgattcattt tcatccgtgc cgatgccgag atacatcgga
     1621 gtgctcattg aagaggggat aaagaccggt tcgccggctt gcgcccgaac agcgccgttg
     1681 cggtgcaccc aaacatcttt gccgaaatgg ttttcgcggt taataaacaa gtgaggagaa
     1741 tcatagagca aatcaaattc cggatccttc cccaaaatct tttcaatcgt ttggtcgagg
     1801 ttgtgcgtga gaatcgatcg attcgcgaat ccttggttgc ctgccgcttg atgcgctgtg
     1861 agaaacattt ttccttcagg tgaatcagca tcgtaggcga aaaattcgtc tttgtttttg
     1921 tattcggcga ttttcttcgc gattttcttg tagatgtttt ttttaccgga tcgccaaaac
     1981 aattttccaa gctcaagcac gatgcgctgg ctcaaatgtt cttttttttt cggagtatac
     2041 agatacgatg catactgtcc caaaagacca gaacctgtgt gaaacatgaa aatatattgt
     2101 ccttttttga gtccgaattt ttccgccatt tcgggatctt ttatttcgga tattttcatc
     2161 agatcaagaa agtgattgcc ggccgctcca aggatcccga gacgaaattt tccgatccac
     2221 aaaaacagct ggggaatggc gtccgtgata tcttgttggc ttatttcctg atcaaagaaa
     2281 tttccattct tgaatgtgtt ttcgatttcg tttttcgtcc gcgtgtgaaa atattccgtc
     2341 accggcttga gtccgtagcg gcaaatatcg actactagcg aatagggaac cggcgttccg
     2401 acataggcct ttgtcggaac cggcttcaaa agggcattga acaatttgtc gatattttcc
     2461 ggggtcagat cggcatcagt cagatttgtt ttcaaaaacc gcattccgca gttgggcgct
     2521 gtatcattga tctgaggcag aagatatttt tcgcttgcaa caactgttcc cgtcggattc
     2581 ttgcggcctt ttttgtcatg aacgtccgaa agcgccgcaa tgtgatggaa aacatattct
     2641 tgcgaagcgg tattttcaag ctgcgcgagc gttttgtcgt ttggtaaaag ttcgtcgctg
     2701 gcatgcaaaa tcgccgggac tttcattctg tccgtctgaa atttcttttt gtgagtggat
     2761 agagattcaa tcttcatgag ttttcataaa cctcttcgta ctctttcaca attttcgtta
     2821 tatcaaaatt atcgcggaca aaatcgatgc cacgccgcgc cagatcattt cgcacttcag
     2881 ggttatcgca taaatataaa attttttgtt tcaaaacttc gcggttgccg ggcggaataa
     2941 gaatactgtt ttcattgttc aagaaatatt tgagcctttc aagactgctt gcgatcaccg
     3001 gcttgccgca agccatcgct tcaatgacgg ccagcggaat atcaaacttc ccggccatcg
     3061 tccgcgcggg aaaaatggag atgtccgaaa ggttgtatat ctcttccata gtataactgc
     3121 cgtcatcgat aaatgccact ctgcctagaa cgtcttcttt ttttagtttg tcgataattt
     3181 ctcgcttttt ctcggcatct ttttggtttt tcacccgcac ggcgagatga agcttgaaat
     3241 tttgatatcg ttttgctagg cctgaaaaag attcgataat atcgtccatg tccccaagcc
     3301 gcacatattc tccggtataa ttgatcacga aatctttttc gctcgttgca aattttttca
     3361 tcaattgctg attttttgga gcaggggaat ataaattcac atcaatcccg ggataaattc
     3421 tttttattct attaaatccc attttttcca acttctcttt cgcgtagtcg gaataagtga
     3481 tgatgaagtc cgcatgaatt atttttttca atttttccgc
//
LOCUS       DATKGS010000144         2873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_85700, whole genome shotgun sequence.
ACCESSION   DATKGS010000144 DATKGS010000000
VERSION     DATKGS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85700"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..638
                     /gene="rnpB"
                     /locus_tag="VMQ48_03595"
     ncRNA           170..638
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VMQ48_03595"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            650..2068
                     /locus_tag="VMQ48_03600"
     CDS             650..2068
                     /locus_tag="VMQ48_03600"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HUD09146.1"
                     /translation="MKKSELFFAAILVPADFLMIVFAAAVSFWLRFTPGIIEIKPVLF
                     QFPFFSYLKIVAIVAPFFLLIFALQGLYSLKTTRTFWREFLQITFAISVGMTLVILAI
                     FLQREWFSSRFIILSAWGLSIVFISLSRYAVNLTQKILLIYKGIGRHRLVLIGKSGYC
                     DTVCHEYKKNPGHGYKIIAQEPTIDIDNLRHIQKERGIDEIFVCDPDIPMKDLREVSD
                     FCHRNRIEFKFVPAILQSISTNFEVKALFDEPVIEIKNTPLDGWGKIAKKIFDFVGSL
                     FGLIITSPLLLVTAIAIRLDSRGPIIFKNERVGHHGNFNVYKFRYFKIEYCTGIQNPN
                     WEKALELEKKLIETQSVRKGPLYKIKNDPRKTRVGRIIEALSIDELPQIFNVLKGDMG
                     LVGPRPHQPREVEKYEMWQKRTLSIKPGMTGLAQISGRSDLSFTDEARLDIYYIENWS
                     LWMDIEILFKTFFVLLKKRKNL"
     gene            2065..2661
                     /locus_tag="VMQ48_03605"
     CDS             2065..2661
                     /locus_tag="VMQ48_03605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HUD09147.1"
                     /translation="MSQDFIHLLPYWGYPLMFVLMFFEGPFATMIAAFLASQGFFNIG
                     IVFVLSVSADVLGDIGLYHIGYYGGPKIMEKAQKFLKISNATIEKLKNKFHQNSSRVI
                     FYVKSTTGLCAITFILAGTLRMKFLKFIKYSFLGGLVWSSFIVILGYFFGYAAAQISQ
                     YIKYAGVAIFAAAIIAVVVISLIKKRESAEILGNGNGN"
BASE COUNT          789 a          615 c          655 g          814 t
ORIGIN      
        1 acccatattc cgcgtgaaaa gaactcggaa gcggatcgat tggccaattt ggcacttgac
       61 gaagccactc gcgcccaaag ccggatgttc tagattggct tctacaaggc aatttatagc
      121 tatattgccc tattgacaaa tattttaaaa aatattatag tattaagtta gttggggctg
      181 gataatcgcc ttgttccgca aggagctggg aggaaagtcc ggacacctgc cggtgctctg
      241 cattggcaaa aagagcaatg gataacgccc atcgggcgca ttgaaagatg cgcctaggga
      301 aagtgccaca gagactatac tagccaatcc cgatttttga ctatcgagag atccgaccgc
      361 gcggccacaa aggaatgcgg acggatctcg tcaaaaatcg ggattggcca aaggtgaaaa
      421 gccgattgat gttcaatgtt tactcacgtg aggcattgag ctgcaattgg agccggccgg
      481 caacggctgg aacttggcaa accctgcttg gtgcaagttt gtacttcggc gcaagtcgga
      541 ggaaacgctc gagcttaata gcaatattga gcctagataa atgattgtca tcctgcttcg
      601 gcgggacaca gaatccggct tataggcccc agctaactct atatttgcta tgaaaaaatc
      661 agaacttttt tttgccgcaa tacttgtccc tgccgatttt ttgatgatcg tctttgcggc
      721 agcagtttca ttttggctcc gtttcacgcc cggtataatc gaaattaagc cggttttgtt
      781 tcagtttcca tttttttcat atctcaaaat agttgccatt gtggcgcctt tttttctgct
      841 tatttttgcg cttcagggac tatactctct gaaaacaacc cgtacttttt ggcgggaatt
      901 tttgcagata acttttgcta tttccgtcgg aatgacgctt gttattctgg ctatttttct
      961 ccagcgcgaa tggttttcct cgcgtttcat cattctttct gcctggggac tgtctatcgt
     1021 attcatcagc ttgtcccgct acgccgtgaa cctgacgcaa aagatactcc tgatctacaa
     1081 gggaatcggg aggcaccgcc ttgttctcat cggaaaaagc ggatattgcg atacggtttg
     1141 ccatgaatac aaaaaaaatc cgggacacgg atacaaaata atcgcacagg aaccgacaat
     1201 tgatatcgac aacctccggc atattcaaaa agaaagaggt attgatgaaa tttttgtttg
     1261 cgatccggac attccaatga aagatctccg cgaggttagt gatttttgcc atcgcaaccg
     1321 gatagaattc aaatttgtcc cggcaatcct gcaatcaatc agcacgaatt tcgaggtcaa
     1381 agcgcttttt gacgaaccag ttattgaaat caaaaatact cccttggacg gatggggaaa
     1441 aatcgctaaa aaaatatttg acttcgtcgg atcacttttc ggccttatta taacctcgcc
     1501 gttattgctc gtaaccgcaa tcgcaatcag actggattct cgcgggccga ttattttcaa
     1561 aaacgaacgg gtggggcacc atggaaattt caatgtttat aaattccgat attttaaaat
     1621 cgagtactgc acgggcattc aaaaccctaa ttgggaaaag gctcttgaac tcgaaaagaa
     1681 actaattgaa actcaaagtg tccgcaaggg gccgctttat aaaataaaaa acgatccgcg
     1741 aaaaacccgc gtcggaagga tcattgaagc tctgtcgata gacgaattgc cccagatttt
     1801 caatgttttg aagggcgata tgggactggt tggtccgcgt ccgcatcagc cgcgcgaagt
     1861 ggaaaaatat gagatgtggc agaaacggac gctctccatc aaaccgggta tgacggggct
     1921 cgctcaaatt tccggccgat cagatttgag tttcactgac gaagcccgtc ttgatatcta
     1981 ttatattgaa aactggtcgc tgtggatgga tattgaaatt ttgttcaaaa catttttcgt
     2041 gctgcttaag aaaagaaaga acctatgagc caagatttta tccaccttct cccatactgg
     2101 ggatacccct tgatgtttgt tttgatgttt tttgaggggc catttgcgac gatgattgct
     2161 gccttccttg cttcgcaggg attttttaat atcgggatcg tttttgtttt gagtgtttct
     2221 gcggacgttt tgggtgatat tggcctgtat cacatcggct actatggtgg tccgaaaatt
     2281 atggaaaaag cgcagaaatt tttgaaaatt agcaacgcaa cgatcgaaaa attgaagaat
     2341 aaattccatc aaaacagcag tcgcgtcata ttttatgtaa agtcaacaac cggcctttgc
     2401 gccataactt ttatcttggc cggcacgctc cggatgaaat ttttaaaatt tatcaagtat
     2461 tcttttctgg gcggactggt ttggagttca tttattgtca ttcttggcta cttcttcggc
     2521 tatgccgccg cccaaatcag ccagtatatc aaatatgccg gtgtggcaat ctttgcagcg
     2581 gccataatcg cggtcgtcgt gatcagcctc atcaaaaaac gcgaatccgc ggaaattttg
     2641 ggaaatggaa atggcaattg atataacttc ttgatttcat cccgtgaaaa atatcagggg
     2701 cttttttatt gccaaaatcg ggaaaaaaag ctagtattta tctatgaaaa tagctttggt
     2761 tcacgattat ctcatccaat acggcggggc agagagggtc ctggaatgca tcgcggaaat
     2821 ttggccgtac gcgccggttt ataccctgat atatgatgaa aaaaaaacgc acg
//
LOCUS       DATKGS010000145         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_141667, whole genome shotgun sequence.
ACCESSION   DATKGS010000145 DATKGS010000000
VERSION     DATKGS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141667"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..>3135
                     /locus_tag="VMQ48_03610"
     CDS             535..>3135
                     /locus_tag="VMQ48_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09148.1"
                     /translation="MKEKKDKQKIGDIFFGKAGEVFTNVRLFLRFKKIVKIIGKERKA
                     VEGVRGKIAKLYGTTNNSTTENIKVETRQCLVSTEAKKEKNKKADLVFVPLSEIAKNS
                     TYSKNYINFSARQGKLKAKKIKGVWHTTEEWLAEFTEKSQAKKNQFKEKLSKDHGGDK
                     KFVEVAEKAEIGSLASTMEAKLPKQVVKEERIGSFPLLKAELEREMLEKKKAKDNLKK
                     AVAPRAIILRARKSFAKSWNSAKTWVRELGEMEGARQSAKLKKAKLADFWKLDYAKPA
                     AAIIALILLMAIGNITKADLASMGDRGKKQIYFTYNAGVDTVAKIADSVNKGAGKSIA
                     KVKIGKESFVKIAEDVLRREKIIDFEKKTGIALKGNEESKGNLVNQEQGGAVLGEESG
                     RDAINRVSTTGGAGEGVVLAAATGAAQTNIGDIEVSAYLMDGANKELPNGEYDVRFGI
                     YTTDRTEADPYPSDTDKGTRVWEETQTVTVENGLLKTYLGATTPIPASFNFAASNYYI
                     GIRVGEDGEMVPRKRIGAVPLARTAMNATTVNGLTVGNAAGNVPTSNGTLNTNLNADL
                     LDGLHSTAFQVAGSYQPAGTYDNYVAWKLQAFTADAGQKITMNKGAIFTGANGIVTTR
                     TLNTLTIAPTYGSTDNTIAQGSTVITVNTDGNLQGGGSGTAGGGISLTLNTVDNPTFT
                     TAYVSTLDLGTNTITDQNLNGDWNFNAGVLSNIASATVTGNFNLTASSNLIFGGTTSL
                     AATTAPNNSGAYLVGVNDEFANSNSTNVQAVLKDLDAAIAGGGGPGTVWTLSAGNIHP
                     TDNTNSLAVGTASQFQVSNTGNVTGGTYNGVTLSGNGTLATGTGPYTLTLTGSSNLNQ
                     NLLTTSSPTFN"
BASE COUNT          985 a          686 c          838 g          626 t
ORIGIN      
        1 attttgagat ttcaattttg aattatttac ctgtggataa ctaaaaaagt agacgcaaaa
       61 aaaatttcga cttgacaaga aattttgcga gcgaataaaa aattttagca aataaaattt
      121 caagttgacg ctgaagcgaa aaaaatagaa aaatcataaa tccgattttt ggcgtaaaac
      181 caaaatccga tattttcaat aatttgcgaa gcaaataatg aatatcggtt tttttgttgg
      241 attggattag aaaattggtt ggattgggaa aatgaaggta atttggagag attaaattcg
      301 gtataatttg ggaaatcgcg gttaaaaaat tttttgaaaa aagtcggcca ttttttgaca
      361 tgggacttgc aagggtttac aggaaaaata taataaatac ataagctgtt aaatgtcata
      421 tacaaagcaa ataacaggag cggcggtctc atcccaaccc tctccctgaa aaaggaaagg
      481 gaataagaaa aacaaactcg attaaaactg atgtaaaaag gatataacgc ggtcatgaag
      541 gagaaaaaag acaaacaaaa aattggggac atatttttcg gaaaggccgg ggaagttttc
      601 acgaacgtcc ggcttttttt gcgcttcaaa aaaatcgtca agatcatagg caaggagaga
      661 aaagcggttg aaggcgtgag agggaagatc gcaaagttgt acggaacaac aaacaacagc
      721 acaaccgaaa atatcaaagt agagacgagg caatgcctcg tctctacgga ggcgaaaaaa
      781 gaaaagaaca aaaaagccga tttggttttt gtcccgcttt ccgagatcgc gaaaaattcg
      841 acgtactcga aaaactatat caacttctcg gcgcgccagg gaaaactcaa agcgaaaaaa
      901 ataaaaggcg tttggcacac gactgaagag tggctcgccg agttcacgga aaagtcgcag
      961 gcgaagaaaa accaattcaa ggaaaaactt tcgaaagatc atggggggga taaaaagttt
     1021 gtggaagttg cggagaaagc ggaaattgga agcttagctt ccacgatgga agctaagctt
     1081 ccaaagcagg tcgtgaaaga ggaaaggatc ggttcgttcc cgttgctcaa ggcagagctc
     1141 gagagggaaa tgctcgaaaa gaaaaaagca aaagataatt tgaaaaaagc tgtcgctccg
     1201 cgggcgatca ttctccgggc gaggaagtct ttcgcaaaga gttggaacag cgcgaaaacc
     1261 tgggttcgcg agctgggcga aatggagggc gcgcgccaaa gcgccaaact caaaaaagca
     1321 aaactcgcgg atttttggaa actggattat gcgaaaccgg cggcggcgat cattgcgctg
     1381 atccttttga tggcgattgg gaatatcacg aaggcggatt tagcgagtat gggagaccgg
     1441 ggaaagaaac aaatatattt tacctacaac gcgggcgttg acacggttgc gaaaatcgcg
     1501 gatagcgtga ataaaggcgc tgggaagagt attgcgaaag tgaagattgg aaaagaaagt
     1561 tttgtaaaga tcgcggagga tgttttgcgg cgagaaaaaa taatcgactt tgaaaagaaa
     1621 acgggaatcg ctttgaaggg gaacgaggag agcaagggaa atttggtcaa tcaagagcag
     1681 ggaggcgcgg tgctgggaga ggaaagcggt agagacgcga ttaatcgcgt ctctacgacg
     1741 ggaggcgccg gagagggagt tgtcctggcg gcggcgaccg gggcagcgca gacgaatatt
     1801 ggcgacattg aagtctcggc ttatctgatg gacggagcaa acaaggaact tccaaacgga
     1861 gaatatgacg tgcgctttgg aatatatacg accgatcgga cggaagcgga tccctatcct
     1921 tccgacacag acaaaggaac gcgcgtctgg gaagaaacgc aaacagtgac cgttgaaaac
     1981 ggactcttga aaacctatct cggtgcgaca acgcccattc cggccagttt caattttgcg
     2041 gcttcgaact actatatcgg catccgggtc ggcgaggacg gagagatggt gccgcgcaaa
     2101 aggatcgggg cggtgccgct cgcgcgaacg gcgatgaacg ccaccacagt gaacggcctc
     2161 acggtcggca acgccgccgg caacgttcct acttccaacg gaacattgaa cacgaacctc
     2221 aacgcggatc ttttggatgg actgcactcg acggcgtttc aggtcgcggg ttcataccag
     2281 ccggcgggga cgtatgacaa ttatgtcgct tggaaattgc aagcattcac ggcggacgcg
     2341 gggcaaaaaa taacaatgaa caaaggcgcg attttcacgg gcgccaatgg gattgtcaca
     2401 acgcggactt tgaacacgct cacgattgcg ccgacctatg gttcaaccga caacaccatc
     2461 gcccaaggca gcacggtcat caccgtgaac acggacggaa atttgcaagg cggcggatcg
     2521 ggaacggccg gcgggggaat cagcttgact ctcaacaccg tcgacaatcc aaccttcacg
     2581 actgcctatg tttccacgct tgatctcgga acgaacacaa tcacggatca aaatctgaac
     2641 ggcgattgga atttcaatgc gggagtgctt tcaaacatcg ccagtgcgac cgtcaccgga
     2701 aatttcaatt tgaccgcgag ttcgaatctc attttcggcg gaacaacgtc gctcgccgcc
     2761 acgacggctc cgaacaattc cggcgcctat ctcgtcgggg tgaacgatga atttgcgaat
     2821 tcgaactcga cgaatgtgca agcagttctc aaggacttag acgcggcgat tgcgggaggc
     2881 ggcggaccgg gaacagtttg gactctttcc gcgggaaata tccacccgac ggacaatacg
     2941 aacagcctgg ccgtcggaac cgccagccaa tttcaagttt caaacactgg caacgtcacc
     3001 ggcggcacct acaacggcgt caccctctcc ggcaacggca ccctcgccac cggaaccggt
     3061 ccttacactc tcactctcac cggcagctcg aacctgaacc aaaatctcct cacgacttct
     3121 tctcccactt tcaac
//
LOCUS       DATKGS010000146         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_70832, whole genome shotgun sequence.
ACCESSION   DATKGS010000146 DATKGS010000000
VERSION     DATKGS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70832"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VMQ48_03615"
     CDS             complement(<1..1032)
                     /locus_tag="VMQ48_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09149.1"
                     /translation="MLSSQDQLKNQIESAEEILILTAEKPTRDAVAASWALSHLLQSL
                     GKKPTVLQMNAADSFSYLTPPEKMESEIKGARDFVLSFDTKRNKITDFRTENKINSFD
                     IYITPEKAAIDPRDFSFIPAKFKYDLVIVLGCQSLESLGNLSEKNSDLFFEVPVANID
                     NASANENFGQINLVDITASSISEILTGLFKSLWASLASKNIAQCLLTGLIAATHNFQS
                     KNTTPQTFLAASWLIEKGANQQEIVRSLFKTNSFSFMKLWGRVMARLQWDEDLKIAWS
                     TLSLEDFVQSRTKPEELPLVLEKVKDNFAAGKYFAILFSETLDRSIALVRNNDSESLK
                     NIQKLLGGEI"
     gene            1521..2237
                     /locus_tag="VMQ48_03620"
     CDS             1521..2237
                     /locus_tag="VMQ48_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HUD09150.1"
                     /translation="MPTLDPIRIGTVRRCAIEVHSRDTGKAVASIMVVIIVATNPRFC
                     LRAGLPRSRSVHLPPTFFFAKNDIYATIKSMNSKGIFITNSSKQTQKMGEILAKELKG
                     GEIVCLTGELGSGKTTFAQGMLRGFKVKGPHTSPTFVVMKKYKAKFSIFNFQFSNNSQ
                     ISKLKKQKKYQIPNIYHIDAYRVGAKDILDLGWEEIIFGKNNIVIVEWAERIKKIIPR
                     NALWIKFKHIENIKRQIAVK"
     gene            2385..>4637
                     /gene="polA"
                     /locus_tag="VMQ48_03625"
     CDS             2385..>4637
                     /gene="polA"
                     /locus_tag="VMQ48_03625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HUD09151.1"
                     /translation="MKKKLVLIDSNALIHRAYHALPPLTTKKGEIVNAVYGYTSVLLN
                     VLAKFKPEYIVAAFDLKEPTFRHKEFKDYKATRVKAPDDLYAQIPRVKELVKSFNIPI
                     AEKEGYEADDLLATFARKTEKLHPDVEVIIVTGDLDTLQLVSDQTKVFALRRGMSDSI
                     MYDEKAIFERYGLKPDQMNDYKGLRGDPSDNLPGVKGVGEKTASELLQKYGTLENIYK
                     NLPEIKEGVRKKLEKDKVQAYFSKRLATLVDNVPVDFDLEKARVEDFSRVKVINLLRE
                     MEFFSLLRRLPGYEDHMKDIEAEKSVQEGESGKIAYKKIQSNELPHLIGGQAQTKKHI
                     SIITDGEIRGTLNGIALGVDSRSAFYISKIDEKIKEIFSDEKIKKIGFDLKKDWRILD
                     ENKTVLKGMDFDVKIAAYLLKSGSNLSWEDLVMQELGVIVEKEDKKKGQATLVFQEDE
                     KAFEKICRASTLNFALKEKLEKDLERISSEQIIDGREKTIKDLFENLEMPLIEILSRM
                     EKVGIKINKIVLSGISEKLDRRVEKLDKEIQKMAGEKFNVNSNVQLREVLYEKMKIPT
                     TDIKKGKTGYSTASPELQKIKSLHPVIEKIEEYRELFKLKTTYLDTLPNMTDKNSRLH
                     TTFNQAVTTTGRLSSSEPNLQNIPIRTELGQVIRTAFEAKSGHRLVSADYSQIELRIA
                     AHLSGDVRMITTFLSGEDIHTATAARVNKIDLDKVSKKMRSAAKALNFGIIYGMSTFG
                     FSQSAGVDQEE"
BASE COUNT         1392 a          930 c         1140 g         1175 t
ORIGIN      
        1 aatctcacct cccaacaatt tttggatatt tttgagtgat tctgaatcgt tgttccggac
       61 gagcgcgatg ctccggtcga gcgtttccga aaagaggatc gcgaaatatt ttccggccgc
      121 gaaattgtct ttcacttttt cgagaacgag cggtagttct tccggtttgg ttcggctttg
      181 gacaaagtct tcgagcgaaa gggtggacca ggcgattttc agatcttcat cccactggag
      241 gcgcgccatc acacgtcccc aaagcttcat aaaggaaaaa gaattggttt tgaaaagact
      301 cctcacgatt tcctgctggt tggcgccttt ttcgatgagc caagaggcgg cgaggaatgt
      361 ttggggagtc gtgtttttgc tttggaaatt gtgcgtggcc gcgatgagac ccgtcagaag
      421 acattgggcg atgtttttgc tcgcaagact cgcccagagg ctcttgaaaa gtccggtgag
      481 tatttccgaa atggacgaag cagtgatgtc gaccaaatta atctgcccaa agttttcatt
      541 tgcggacgcg ttgtcgatgt ttgcgaccgg cacttcaaaa aaaagatcac tgtttttttc
      601 actcaagttc ccgagagatt caaggctctg gcagccgagg acgatcacga gatcatattt
      661 gaatttggcg ggaatgaaag aaaaatcccg ggggtcgatc gccgctttct cgggggtgat
      721 atagatatcg aacgaattaa tcttgttttc tgtccggaag tccgttattt tattccgctt
      781 cgtatcgaac gaaagaacaa aatcgcgggc gcccttgatc tcactttcca ttttttccgg
      841 tggagtgaga taggaaaaag aatcagcggc attcatctgt aaaacggtcg gtttcttccc
      901 gaggctttgg agaagatgcg aaagcgccca gctggcggct actgcgtcgc gggttggttt
      961 ttctgcggtg aggatcaaaa tctcctccgc ggattcgatt tggtttttca attggtcttg
     1021 agaagagagc atgtaacata taacatgtaa catgtaacaa attattttgt gtttcgtgtt
     1081 acatgtttcg tgttttgtga tggcgtggtt ttatagaata tcaaacgagg caaagagtgt
     1141 caattgacag aatattccct tctttgtgaa acattggatt ttggcgtaga ataggcatat
     1201 tcctgattgt ttcacggtgg ggttgacaat tggccgtttt gggagtaaaa tggacaattg
     1261 aaataaaaag aaaaacccta gcacataaag gccgaatggc cgaaggagag aggatatgtt
     1321 cctgaaaaag actttgcaag ttgcggctgt ggttgcggtc ctggtgatct gttgcttagg
     1381 ctgcgcggtt gatcagtatg gtcggccggt cggggtatat ccagtggtgg tagtcgacac
     1441 ataccctgcc gatgtatacc cctattattg gggtggatat tattactatc catactggac
     1501 cggatcagta tgggcttaca atgcctactc tcgaccccat ccgcattgga acggtccgcc
     1561 gttgcgccat agaggtccac agccgagata ctggcaaggc ggtggcaagc attatggtgg
     1621 tgatcatcgt cgccactaat ccacgattct gcctacgggc gggattgcca agaagtcgga
     1681 gtgtacactt gcctccgact tttttctttg ccaaaaatga tatttatgcg acaataaaat
     1741 caatgaatag caagggtatt tttattacaa atagctccaa gcaaacccaa aaaatgggtg
     1801 agattttggc gaaagagctg aaagggggcg aaatcgtctg tctcacgggc gaattggggt
     1861 ccgggaagac gacttttgcg caaggcatgc ttcgcgggtt caaagtgaag ggacctcata
     1921 caagccccac ctttgtcgta atgaaaaagt ataaagccaa attttcaatt ttcaattttc
     1981 aattttcaaa caattctcaa atttcaaaat taaaaaaaca aaaaaaatac caaataccaa
     2041 atatttatca cattgatgcg tatcgtgtcg gggcgaaaga tattctcgat cttggctggg
     2101 aagaaattat tttcggcaaa aataatattg tgatcgttga gtgggcagag aggataaaaa
     2161 aaattattcc gcgtaatgca ctttggataa aatttaaaca tatagagaat attaaaaggc
     2221 agatagctgt aaaataattt tcaattttca atttcaaatt ttaaatgaat ttcaaatgac
     2281 cgaatttcaa atgttttaaa attatgtcat ttagaaatta aaaattgatt taaaatttat
     2341 aatttgaaat ttaaaattca agataatttt ccatcagcaa catcatgaaa aagaagcttg
     2401 tgttaataga ttccaacgcc ctcatccacc gcgcctatca cgcccttcct cctctcacga
     2461 ccaaaaaggg agaaatcgtg aacgcggtgt atggctatac gtcggtgctt ctcaacgtgc
     2521 tcgcgaagtt caagcctgaa tatatcgtgg cggcgttcga tctcaaagaa ccaaccttcc
     2581 ggcacaagga gttcaaggat tataaagcga cgagagttaa ggccccggat gacctttatg
     2641 cgcagattcc gcgagtgaag gagttggtca aatctttcaa tattcccatt gccgaaaaag
     2701 aaggatacga agccgatgat ctgctggcca ctttcgcgcg caaaaccgaa aagctccatc
     2761 cggatgttga agtgatcatc gtcacgggcg atctcgacac gctgcaactg gtaagcgacc
     2821 agacaaaggt tttcgctttg cggcgcggaa tgagcgattc gataatgtat gacgaaaagg
     2881 cgatctttga acgatacggc ctcaagcccg accaaatgaa cgactacaaa ggtctccggg
     2941 gcgatccgtc ggacaatttg ccgggcgtga agggcgtcgg tgaaaaaaca gcgtctgagc
     3001 ttctccagaa atacgggacg ctggaaaata tctacaaaaa tctgccggag atcaaagagg
     3061 gagtccgcaa aaaactcgag aaagacaaag tacaggccta tttttcaaag agactggcga
     3121 cattggtgga caacgtgccg gtcgattttg atcttgaaaa agcgagggtc gaggattttt
     3181 cgcgggtgaa agtgatcaat cttctgcgcg agatggaatt tttcagcttg ctgcgacgac
     3241 tccctgggta cgaagaccat atgaaagata tcgaagcaga aaaatctgtt caagaaggag
     3301 aatcagggaa aatagcttac aaaaaaattc aatcaaatga attgccccat ctcatcggcg
     3361 ggcaagctca aacaaaaaaa catatctcca taattacgga tggagaaata aggggaacat
     3421 tgaatggtat agcgctgggc gttgactcaa gatcggcttt ctatatttcc aaaatcgacg
     3481 aaaaaataaa agaaatattc tctgatgaaa aaatcaaaaa aataggcttt gatctcaaga
     3541 aagactggcg aattttggat gaaaacaaga cagttctcaa ggggatggat tttgatgtca
     3601 aaatcgcagc ctatcttctc aagtccgggt caaatttgtc ttgggaagac ttggtgatgc
     3661 aggaactcgg cgtgatagtc gaaaaagagg acaagaaaaa aggccaagcg acgcttgttt
     3721 ttcaggaaga cgaaaaagca ttcgaaaaaa tatgtcgcgc atcgactctc aacttcgcgc
     3781 tcaaggaaaa attggaaaaa gatttggaaa ggataagctc tgagcaaatc atcgatggcc
     3841 gggagaagac aatcaaggat cttttcgaaa accttgagat gcccctgatc gaaattctct
     3901 ctcggatgga gaaagttgga atcaagatca ataaaatagt ccttagcggc atttcggaaa
     3961 aattggatcg gcgggtggaa aaattggaca aagaaattca aaaaatggct ggggaaaaat
     4021 tcaatgtgaa ctcaaacgtc caattgaggg aagtgctcta tgaaaaaatg aaaataccga
     4081 ccacggacat caaaaaaggg aagaccggat actcgactgc ttctccggag ctgcaaaaaa
     4141 ttaagtcgct ccatcccgtc attgaaaaga tcgaggaata cagggaactt ttcaagctga
     4201 aaacaactta tctggatact ttgccgaata tgacggacaa aaattcccgt ttgcatacga
     4261 ctttcaacca ggcggtcacg acgacgggaa gattgtcatc gtccgaaccg aatcttcaaa
     4321 atattcctat ccgcacagag ctgggacagg ttatccgtac ggcgtttgaa gccaagtccg
     4381 gccatcggct ggtgagcgcg gactattccc agattgagtt gcgcatcgcg gcgcatttgt
     4441 ccggggatgt gcgaatgatc acgacctttt tgagcggcga agacattcac acagccacgg
     4501 cggcgcgggt gaacaagatt gatctcgata aagtgagcaa aaaaatgcgc agcgcggcca
     4561 aggcgctcaa ttttgggatc atatatggga tgagcacttt tggtttttcc cagtcggcgg
     4621 gcgttgatca ggaagaa
//
LOCUS       DATKGS010000147         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_71035, whole genome shotgun sequence.
ACCESSION   DATKGS010000147 DATKGS010000000
VERSION     DATKGS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71035"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..406)
                     /gene="rplM"
                     /locus_tag="VMQ48_03630"
     CDS             complement(2..406)
                     /gene="rplM"
                     /locus_tag="VMQ48_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HUD09152.1"
                     /translation="MEKYYLINCENFILGRMSTKIAFLLQGKDKPQYAPNKIGDCFVI
                     VTNTDKLNVSGRKKSDKMYHSFSGYPGGITSRKLEDVLERDSRKIVWESVYGMLPKNK
                     LRDLMMKKMLIFKDDKHGVHNVEIEEIKAQKA"
     gene            complement(406..777)
                     /gene="rplQ"
                     /locus_tag="VMQ48_03635"
     CDS             complement(406..777)
                     /gene="rplQ"
                     /locus_tag="VMQ48_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HUD09153.1"
                     /translation="MKHLKTGRKFSREKKQREAMLKIMLGDLFIKRKMKTTLAKAKEL
                     KMIAEKMIGQMKDPQSLRLLKSRLPRNVTPAIIHDLAAKTASRNSGYLRVLKCGARQS
                     DSAQIAIIEIIEDKIAIESKK"
     gene            complement(791..1768)
                     /locus_tag="VMQ48_03640"
     CDS             complement(791..1768)
                     /locus_tag="VMQ48_03640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HUD09154.1"
                     /translation="MQKISLPSAPKYTEIGKNIGKIEIDGCYPGYGTTLGNSLRRVLL
                     SSLVGTAATSVKIKGVSHEFSTVPGVLEDVIQIILNLKKVRFLSHSDEPVKLTLKEKG
                     VKKITAAQIQCPSDVEVISKDLPIATITDKKGELELEIEVQLGLGYVPVEQQVREKKE
                     IGLIAIDAVFTPVRRVNYSVENTRVGKRTDYDKIVLEIETDGSVSPQEAFQKAVETIT
                     EQFSAVKDFEVEKEIVSNEEENIQEQTPVVAEKDEAEKNDAKAKGVSIESLSFSTRTM
                     NVLEKSNIKTIEDLIAMTEEELMNIEGMGQKGAKEIKKAVGNFGLTLKS"
     gene            complement(1817..>2353)
                     /gene="rpsD"
                     /locus_tag="VMQ48_03645"
     CDS             complement(1817..>2353)
                     /gene="rpsD"
                     /locus_tag="VMQ48_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HUD09155.1"
                     /translation="KPAPPGMHGAKSRRGNKSEYGQQLAMKQRIKRIYAVLERQLNKY
                     FQEVSNKQGVTGDLLMQKLELRLDNVVYRAGFASSRRQARQLVKHSLFLVNGKSANIP
                     SIELKNGDKVSIKESKSDKNYFKQVLAAIKESKGAGLPNWMQMDAEKVILEIKGRPTR
                     DDFGAGIDAQMVVEFYSR"
BASE COUNT          532 a          586 c          449 g          786 t
ORIGIN      
        1 cttaagcttt ttgcgccttt atttcttcga tttcgacatt gtgcacgccg tgcttgtcat
       61 ccttgaagat gagcatcttt ttcatcatca ggtcacgcag cttatttttt ggaagcatgc
      121 catagacgga ttcccaaaca atcttgcgcg aatctctttc caagacatcc tcgagtttcc
      181 gcgatgtgat tcctcccgga taaccggaaa acgaatggta cattttgtcg ctttttttcc
      241 ggccggaaac attcagtttg tcggtatttg tgacaataac aaaacaatca ccaattttgt
      301 tcggggcata ctggggctta tccttgccct gcaaaagaaa ggcgatcttg gtcgacatgc
      361 ggcccaaaat gaaattttca cagttgataa gataatattt ttccattatt ttttgctttc
      421 aatggctatt ttatcttcaa taatttcaat aatcgctatc tgcgcgctat ccgattggcg
      481 cgcgccacac ttcaaaactc tcaagtatcc actgtttcgc gaagcagttt ttgccgccaa
      541 atcatgaatg atcgccgggg tgacattgcg cggcaatctt gacttcaaaa gccggagtga
      601 ctgcggatct ttcatttggc cgatcatttt ctccgcgatc attttcagct ccttagcttt
      661 agcaagagtt gtcttcatct ttctcttgat gaacaaatcg cccagcatga tcttgagcat
      721 ggcttctcgc tgcttttttt cccggctgaa ttttctgcct gtcttgagat gcttcattgg
      781 attgaaattc ttatgatttc agcgttaaac caaaattgcc tacggctttc tttatttctt
      841 tggctccctt ttgccccatt ccttcgatat tcatcaattc ttcttccgtc attgcgatga
      901 gatcctcgat ggttttgata ttgcttttct caagcacgtt catggtccgg gtggaaaacg
      961 agagactttc aatcgaaacg cctttagctt tggcgtcgtt tttttcagct tcatcttttt
     1021 ccgccacaac aggcgtttgc tcttgaatat tctcttcttc attggataca atttcctttt
     1081 caacctcgaa atcttttacc gcgctgaact gttccgtaat cgtttcaacc gctttctgga
     1141 acgcttcttg aggagaaaca gacccatctg tttcgatctc gagcacaatc ttgtcatagt
     1201 cggtcctttt tcccacacgc gtattttcaa ctgaataatt gactctgcgg acaggcgtaa
     1261 acacggcgtc aatcgcaatg agtccgattt cttttttctc gcgcacttgc tgttcgaccg
     1321 gaacatagcc caatcccaac tgaacttcga tttccaactc caactcacct tttttgtcgg
     1381 taattgtcgc aatcgggaga tccttgctga tcacttcaac atccgaagga cattggatct
     1441 gagccgctgt gatttttttg acgccttttt ctttgagcgt gagcttcacc ggttcatcgc
     1501 tgtgggacaa aaatctcact ttcttcaggt tcaaaattat ctgaatcacg tcttccaaca
     1561 ctcccggaac agttgaaaat tcatgcgaca cacctttgat cttcacggaa gtggcagccg
     1621 taccgacgag agacgaaaga agcactcggc gaagtgaatt gccgagagtt gttccatatc
     1681 cgggatagca tccgtcaatt tctattttgc cgatgttttt gccaatttcc gtatatttag
     1741 gtgcggacgg cagagaaatt ttttgcattt ttttgaattt aattctaaaa tttaaccttg
     1801 ttgcgtttta taaaaattag cgcgaataga attcaaccac catttgtgca tcaattccgg
     1861 cgccaaaatc atctcttgtt ggtctcccct tgatctctaa aatcactttt tccgcatcca
     1921 tttgcatcca atttggaaga ccagctcctt tgctttcttt tatcgccgcc agaacttgtt
     1981 tgaaataatt tttatccgac ttgctttctt tgatactcac cttatctcca tttttcaatt
     2041 caattgaagg aatattcgca ctttttccgt tcaccagaaa aagagagtgc ttgaccagtt
     2101 gccgggcttg acgccgactg gaggcaaatc ccgcccgata taccacgttg tcaaggcgca
     2161 gctccagctt ttgcatcaaa agatctccgg tcacgccctg cttgttgctt acttcttgga
     2221 aatacttgtt caattggcgc tccaaaacgg catagattcg cttgatcctc tgtttcatgg
     2281 ccaactgctg gccatattcg cttttgtttc ctctgcggct cttggcgcca tgcattcccg
     2341 gcggagctgg ttt
//
LOCUS       DATKGS010000148         9999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_128970, whole genome shotgun sequence.
ACCESSION   DATKGS010000148 DATKGS010000000
VERSION     DATKGS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128970"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VMQ48_03650"
     CDS             complement(<1..671)
                     /locus_tag="VMQ48_03650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUD09156.1"
                     /translation="MTIGIDASRAFVKTKTGIEEYAYRVIKHLRDCLGDQPVVLYLDP
                     RVNEKKYIDFELPQNWKVKFLRAPIFWTQARLSLEMLFRPVDVLFVPAHTVPLIHPKN
                     TIVTIHGLEYEFCPKAYSLFERLYMRWSIRNSCRCARRIITVSENTKNDLMRLYKVPE
                     RKIEVIYEGIGDFEDKAQSEKIKNTTQKLKLLGCGYFLFIGRLEDRKNILGIIEAFEI
                     LKRKY"
     gene            complement(674..1681)
                     /locus_tag="VMQ48_03655"
     CDS             complement(674..1681)
                     /locus_tag="VMQ48_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUD09157.1"
                     /translation="MFIKKIGIDLWTANILVFLPGEGIVVNEPSVVAISLVDNKVLAV
                     GNEAKEMLGRTPEAITAMRPLKDGVIADYRITEAMLRYFINKVAGGFRLFRPEVMVSI
                     PAGVTSTERRAVVDATVKAGAKTSYVVKEPILAAIGAGIPINSAAGNMIVDIGGGTSE
                     VAIISLGGVVAAHSARVGGNKIDQAIADFIKKKHGLAIGERTAEEIKITIGSAIPQMK
                     DEYMEVRGRDLIAGLPKTIRVSANEITEAIQDELREIINAIKKVLQETPPELAADVID
                     KGMILSGGGALLRHLDQLISKTTGVPCYVADEPLLCVAKGTGIALENLDMYKRTIMLT
                     K"
     gene            complement(1819..3072)
                     /gene="murA"
                     /locus_tag="VMQ48_03660"
     CDS             complement(1819..3072)
                     /gene="murA"
                     /locus_tag="VMQ48_03660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HUD09158.1"
                     /translation="MSYFEIIGGKKLNGEIEVKGAKNAALKAVAAALLSREKWTITNF
                     PFIEDTKRILELIEDLGVKVERGKNKVEIQADQIKKRQLDDELAKKLRASIILAGPVL
                     ARKGEVRMHHPGGCVIGKRPIDMFLDGFQKFGAVVKWENNHFRMSAKKLKGAKIFFPR
                     ITVTGTETMMITAVLAEGETILSNAAMEPEIPALAEFLNKCGAKISGAGTPTIRIRGV
                     KSIRGGKMELIPDRIEAGTFAILGALAGGKIKIKNCVPDHVRALLVHMEKAGVKIEEK
                     KNELVVWGAKKLKPVNIATHEYPGFATDIQPPFTLLMTQAKGSSLIHDSIFESRLMFT
                     DALNLMGAHIAMNDPHRATVNGLTKLTGRKITSPDLRAGITLVLAGLIANGKTKIENI
                     YQIDRGYEKIEERLQKLGADIKRIM"
     gene            complement(3360..4664)
                     /locus_tag="VMQ48_03665"
     CDS             complement(3360..4664)
                     /locus_tag="VMQ48_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUD09159.1"
                     /translation="MQWVNQLNRVSSKGRTKIFSLVFVLVVISASAFVVSSSGKTVAS
                     DITVEKVIELTNASRMEAGESALTVNSKLSRSAEAKASDMVANNYFSHTSPAGKTPWN
                     WIQKENYDYIYAGENLAMDFFSAEKMEEAWMASPTHRANILNQNYHDIGTAVKEGIIN
                     GRETILSVVMFGSGDKNSSSADKANKETLNPEKNKDAGKIFPTLSIGEEKKDAALFEQ
                     PTITSPQPGETFSGSEIRVAGRAQPGETVAIFDNGNFVGSAVADPSGWFSSSEENLDE
                     GNHSLALQDKNIFANATTDFYVDREKPAVDFRLYADQNDPSRFFLEASADKNNCTFQF
                     NGESRYVLWGSKALFSIDADKSSAILRVSDQAGNKNFRQINLANYYSGSGNSRNNISD
                     KLAALMSAPENIFADDPGRDAVKNNLGLVPHQFLASRFMDNK"
     gene            complement(4906..6183)
                     /locus_tag="VMQ48_03670"
     CDS             complement(4906..6183)
                     /locus_tag="VMQ48_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09160.1"
                     /translation="MKSYISKITIYFCLVVLFLASDVSLAAQNDAQQQDLKTKINILR
                     NQIQEYQKEISSKGQQEETLQGDIVDLEKDIAKIQLEIQETQLVVQSLDLEIADKEAG
                     IAAMQKEVEAKKKVLAQFMQELYENGNATSVELALGNNTFSDYFFQADSLESFEERTR
                     EIYDQFVFLREGIKKEREDLLNRKEEQMNLRAMQNDQQNTLDSQEQAKNSLLGQTRNE
                     KQALSSQMGKLQDQLNALQALGEPINIDEAISAAKYASGFTKVAPEFLLGVLRVESGL
                     GTNVGGGRYKTDMNPAQWDTFKKICSELGIDPNSVPVSRRACYNSDAKDGCGGWGGAM
                     GPGQFMPTTWLGYKSKVEKVTGEIPANPWDLKDSLVAMGFKLAAVDGVTAGDRAAWAK
                     AAAMYLAGGAWENYSWYSDRVLSYADGFKKIMK"
     gene            complement(6278..6982)
                     /locus_tag="VMQ48_03675"
     CDS             complement(6278..6982)
                     /locus_tag="VMQ48_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HUD09161.1"
                     /translation="MTKNITLDAKIRTKSVGKVADKENIPGILYGRGIENVMVSLDKK
                     KFNDTYEEAGESTVFKLALDGKDERNVLIKDIQKDVLSGAARHVDFYQVRMDEEIEAE
                     IELVFTGESAAVKELGGILVKNMDAIQVKCLPGDLPPEMEVDITPIKTFDDYIYVKDL
                     PFSEKVEVLVDPETVVAMVSEPRSEEELADLETEVKEDVTKVEGVVKEEAPAEGEEAK
                     PEEKKEPASSAGKDKK"
     gene            complement(7042..8148)
                     /gene="rodA"
                     /locus_tag="VMQ48_03680"
     CDS             complement(7042..8148)
                     /gene="rodA"
                     /locus_tag="VMQ48_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HUD09162.1"
                     /translation="MVKNILKMDWILLAAALLLLGLSLAVLYPISHSGNNFVGNDTSH
                     FFRQAIFAVIGIFLFLAFSFTDYRIFRSVSSALFLVGIFLLFAVLVFGKIVNGTSGWI
                     SFGVVNFQPVEIFKLIIAVVLAKYFSLNVKTIREPRHIFISLVPVALSVALIMLQPDL
                     GSALVIVGTWAGVLLLSGVKKRYLIALVIAGLLLGIFSWHFVLKDYQKQRIMVLVSPT
                     SDPHGAGYNVLQSTVAVGSGGVWGKGLGHGSQSQLNFLPEKHTDFIFAVIAEELGFTG
                     VVFVLILLFVILMRLFSIARESRDNFGKLLVGAVAIVFFIQIIVNIGMNIGIAPVAGI
                     PLPLLSYGGSSLVATCAMLGIAESVYRGSRQRYG"
     gene            complement(8177..9031)
                     /locus_tag="VMQ48_03685"
     CDS             complement(8177..9031)
                     /locus_tag="VMQ48_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09163.1"
                     /translation="MTPSSRTIKRLTIVVAYIVIFGLVGTGLYFLFRTKPTCTDKIQN
                     QGETGVDCGGPCAACAEMPKAESLKVVEKAIVPGETGKYDALVKIVNPNSLFGSAKFD
                     YSFNLLDGAGKIIAKSAGSNFVLPGQTKYILAFNIPSDVKPESLDFEISSFEWSKFSQ
                     FEEPDITVYAKEFSLASGGESGFARLTAKMKNQSGFDFHEISAAVVIRGGDGSPIAIN
                     QTNFNDVRAGEEREINVSWNSSFAIDPVTAKIEIVPEVNVFESDNFMKQHGVPGQYGT
                     YNVSGGQQ"
     gene            9163..9612
                     /locus_tag="VMQ48_03690"
     CDS             9163..9612
                     /locus_tag="VMQ48_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09164.1"
                     /translation="MQKQPKIEILRHSLSHVLAAAVFEMFPDARFGIGPAIENGFYYD
                     FDLPRTLIPEDLEILEEKMQAIIKANFKFEKASLSIAEALKDFKKAKQLLKIELIKDI
                     AKNDKNIKKVSIFRTGKFVDLCSGPHIDSTGEISLKAFKLTKISGAY"
     gene            9634..>9999
                     /locus_tag="VMQ48_03695"
     CDS             9634..>9999
                     /locus_tag="VMQ48_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HUD09165.1"
                     /translation="MLQRIYGVAFATDKELRQYLAMMQEAEKRDHRKLGKELGLFIFS
                     DLIGPGLPIYTDKGATIRREIINYSNELQSAIGYQEVHTPNINKADLFKVSGHYEKFE
                     GDMLKVVSHYTDEEYFLKPP"
BASE COUNT         2584 a         2635 c         1920 g         2860 t
ORIGIN      
        1 ttatattttc tcttcaatat ttcaaaggcc tcgattattc ccaaaatatt tttcctgtct
       61 tccagtcttc caatgaacaa aaaatagcca caacccaaaa gttttaattt ttgagttgta
      121 tttttgatct tttcgctttg cgctttatcc tcaaaatcgc caattccttc ataaatcact
      181 tcaatcttcc tttccggcac tttgtacaat ctcatcaaat cattctttgt attctcgctc
      241 accgtaataa tccgtctggc acaccggcat gaattccgaa tcgaccagcg catataaagc
      301 ctttcaaaca gagaataagc cttggggcag aattcgtatt caagtccgtg aattgtgaca
      361 atggtatttt tcggatggat gagcggcacc gtatgcgccg gcacaaacag cacgtccacc
      421 ggacgaaaaa gcatctcaag cgacaaccgc gcctgcgtcc aaaaaatcgg cgcgcgcaga
      481 aatttcactt tccaattttg aggcagctca aaatcaatat attttttttc attaactctc
      541 ggatccaaat acaaaacaac gggttggtcg cccaagcaat cccgcaaatg cttgatcacc
      601 cgataagcat attcttcaat tccggttttt gttttcacga acgctcgtga agcatcgatc
      661 ccaattgtca tatttatttt gtcagcatga tagttctctt gtacatatcc aaattttcaa
      721 gcgcgattcc cgttcctttt gcaacgcaca aaagcggttc atccgcgaca tagcatggca
      781 cacctgttgt cttcgagatc aattggtcga gatgtcgaag caaagctccc ccgcccgaaa
      841 gaatcattcc cttgtcgatc acatcagccg caagctccgg cggagtttct tgcaaaactt
      901 ttttgatcgc attgatgatc tcgcgcaatt cgtcctgaat cgcttccgtg atttcgttgg
      961 ctgaaacccg gatcgttttc ggaagaccgg caatcaggtc gcgtccgcgc acttccatat
     1021 attcgtcctt catttgcgga atcgcgctgc cgattgtgat cttgatttct tccgccgtcc
     1081 gttccccgat ggcaagtccg tgttttttct tgatgaaatc agcgatcgcc tgatcaatct
     1141 tgtttccgcc aactcgcgcg ctgtgcgccg ccacgacccc gcccaaagag atgattgcca
     1201 cttcgcttgt cccgccgccg atatccacaa tcatattccc cgccgcgctg ttgatcggaa
     1261 tgcccgcccc gatcgccgcc aggatcggct ctttgacgac ataggaagtc tttgcccccg
     1321 ccttcacggt tgcatcaaca accgcccgcc gttccgtcga agtgacacca gccggaatgg
     1381 aaaccatcac ttcgggacga aacagccgaa aaccgcccgc cactttgttg ataaagtagc
     1441 gaagcatcgc ttccgtgatc cgataatccg caatcacacc gtctttcagc ggcctcatgg
     1501 ctgtgattgc ctcaggcgtg cgtccaagca tttctttggc ttcgttgccg accgccaaga
     1561 ccttgttgtc cacgagtgaa atcgccacaa ccgacggctc attgacaacg ataccttcac
     1621 ccggaagaaa caccaaaata ttggccgtcc aaagatcaat tcctattttt ttgataaaca
     1681 cgataaaaaa atttaaaaat aaaaaatcaa aatgcaaaat gacagttcaa aatgtaaaat
     1741 ttacccgaat gtcaaaatga acaaaaataa ttcattttaa cattttacat tgtcattttt
     1801 atttttgatt tttgcacttt acattattcg cttgatatcc gctcccagct tctgcagcct
     1861 ctcttcaatc ttttcatacc cccggtcgat ctgataaata ttttcaattt ttgtttttcc
     1921 gtttgcaata agtcccgcca gaaccaaggt gatgccggcc cgaagatcgg gactggtgat
     1981 ttttcttccg gtcaattttg tcaatccgtt gactgtcgct ctgtgcggat cattcattgc
     2041 aatatgagcg cccatcaaat tgagtgcatc cgtgaacatc agacggctct caaaaattga
     2101 atcgtgaatg aggcttgatc ctttggcttg tgtcatcaag agcgtgaaag gaggctgtat
     2161 atccgttgca aatcccggat actcatgcgt cgcgatgttc accggtttca attttttcgc
     2221 gccccagacg accagttcgt ttttcttttc ttcaattttc actcccgctt tttccatatg
     2281 cacaagaagc gcccgaacat ggtcgggaac acaatttttg atcttaattt ttccgcccgc
     2341 aagcgctcca agaattgcaa atgtccccgc ttcaatccga tcaggaatca attccatttt
     2401 cccgcccctt atgcttttca ccccgcgtat cctgattgtt ggcgttcccg cgccggatat
     2461 tttcgccccg cacttgttca aaaattccgc gagcgccgga atttccggtt ccatcgccgc
     2521 gttggaaagt atcgtctcgc cttcagccag aaccgccgtg atcatcattg tttccgtccc
     2581 ggtcacggtg atacgcggaa aaaatatttt cgcgcccttc agtttcttcg cgctcatccg
     2641 gaaatggttg ttctcccatt tcacgacagc tccgaatttt tgaaagccgt cgagaaacat
     2701 atcgatcggc cgcttgccga tcacgcatcc gcccggatga tgcatccgta cttcgccttt
     2761 ccgcgccaaa accggccccg ccagaatgat tgacgcgcgc aattttttcg cgagctcgtc
     2821 gtcaagttgt ctttttttga tctgatcggc ctggatttca actttgtttt tccctcgctc
     2881 cactttcacc ccaagatcct caatcagctc cagaattctt ttcgtatcct caatgaaagg
     2941 aaaatttgtg atcgtccatt tttcccgtga caaaagagcg gctgccactg ccttcaaagc
     3001 cgcatttttc gcccccttta cctcaatttc tccattgagc tttttccccc cgattatttc
     3061 aaaatatgac ataaatattc aattacaagc attggtatta tagatgaaaa agataatatt
     3121 atcaacccaa aacaaaatcc gcccaagttt tttgaaaaaa ctcagacgga tggtttgcca
     3181 ggctgccccg aaaggcaaaa agaaaggaca cttacttttt tgataaaaca cttttttagt
     3241 gtttagatca cccaattttt ttattttgtt tttttaaaga acttctgatt tttgattttc
     3301 ttttacatca catttattaa aaattaataa ttatttatcc ctcctatcat ttggcaatct
     3361 tatttgttat ccatgaatcg tgatgcgaga aattggtgcg gaacgagtcc caaattgttc
     3421 ttcaccgcgt ctctccccgg gtcatccgcg aagatatttt ccggcgcgga cataagcgct
     3481 gccagcttgt ctgaaatatt gtttctactg ttgccgctac ccgaataata gttggccaga
     3541 ttgatctgtc ggaaattttt gtttcctgcc tggtcagaca cgcgcagaat ggctgatgac
     3601 ttgtccgcgt caatggagaa caaggctttg cttccccata aaacatatct cgactctccg
     3661 ttgaattgaa aggtgcagtt gtttttgtcc gcgcttgctt caagaaaaaa tcggctcgga
     3721 tcgttttgat cggcatagag acgaaaatcg acggccggtt tttcgcggtc gacatagaaa
     3781 tcagttgttg cattcgcgaa tatattttta tcttgcagag caaggctatg atttccttca
     3841 tccaagtttt cttctgaaga ggaaaaccat ccgcttggat ccgcaaccgc cgaaccgacg
     3901 aaattgccgt tatcaaaaat tgcgacagtt tctcccggct gggcccgtcc ggcaactctg
     3961 atctcgctcc cggagaatgt ttctcccggc tgcggactgg tgattgtcgg ctgttcaaag
     4021 agggctgcgt cttttttttc ttccccgata gacagcgttg gaaaaatttt tccagcatcc
     4081 ttattcttct ccggattcaa tgtttctttg tttgccttgt ccgcacttga tgaattttta
     4141 tctcccgaac cgaacatcac gacggacagg attgtttcgc gtccgtttat gattccctct
     4201 ttcaccgctg tgccgatatc gtgataattt tgattgagta tgttcgcccg atgcgtggga
     4261 ctggccatcc aagcctcttc cattttttcg gctgaaaaaa aatccattgc cagattctct
     4321 ccggcataga tgtaatcgta attttctttc tggatccagt tccaaggcgt cttcccggcc
     4381 ggagaagtat gcgagaaata gttgttggca accatatccg aagcttttgc ttcggccgac
     4441 cgggacaatt tcgagttcac agtgagcgcg ctctcgccag cctccatcct tgatgcatta
     4501 gtaagctcga tcactttttc cacggtgatg tcagaagcaa ccgtttttcc ggatgaggag
     4561 acaacgaacg cacttgccga aataaccacc aaaacaaaaa ccagcgaaaa tattttcgtt
     4621 cttccttttg agctcaccct attcagctga ttcacccatt gcattcgcaa tttcatccct
     4681 cttcgtaaag ctcaccccgc tatatataat taagactatt taattattcg agaaaaacaa
     4741 aaagcgattt acacccatgt ataaatcacc ctgttcgcgc aaaatccccc tttggaagct
     4801 tgcccgccga ggaggcgaga ctggatctta ctttattatt atataactat ttttagcatt
     4861 agtcaatcgt tccgagatac tttttttcga aaagacttga caaatttatt tcattatttt
     4921 tttgaatccg tccgcatacg aaagaacccg gtccgaatac caagaatagt tttcccaagc
     4981 gccacccgcg agatacatcg ccgccgcttt tgcccaagcc gcccggtcgc ccgccgtcac
     5041 gccgtccacc gccgcgagct tgaaacccat cgccacgagc gagtctttga ggtcccacgg
     5101 gttcgccgga atttctccgg tcactttttc cactttgctt ttgtatccga gccaggtagt
     5161 cggcatgaat tggcccggcc ccatcgctcc tccccatccc ccgcatccat ccttcgcgtc
     5221 cgaattgtag caagcccggc gcgacaccgg cacgctgttc ggatcaatcc caagttcgct
     5281 gcaaattttt ttgaaggtat cccactgggc cggattcatg tcggttttat atctccctcc
     5341 tccgacattt gttccgagtc ccgattcgac gcgcaggaca ccaagcaaaa attccggcgc
     5401 gactttcgtg aaaccggatg catatttggc ggcgctgatc gcttcatcaa tattgatcgg
     5461 ctcgcccagc gcctgcaagg cattcaattg atcctgcaat tttcccatct gcgaagaaag
     5521 tgcttgcttc tcgtttctcg tctgacccag aagagaattc tttgcctgct cttgtgaatc
     5581 caaagtattt tgctgatcgt tttgcatcgc tctgagattc atctgctctt ctttccggtt
     5641 cagcagatcc tctctttctt ttttgatgcc ttcacgaaga aaaacaaact ggtcatatat
     5701 ctctcttgtc ctctcctcga atgattcaag actgtccgct tgaaaaaaat agtctgaaaa
     5761 agtattgttt cccaaggcca attccacgga cgtggcatta ccgttttcat acagctcctg
     5821 catgaattgc gcgaggactt tctttttagc ctccacctct ttctgcatcg cggctatacc
     5881 cgcttctttg tccgcgatct cgagatcaag gctctggacc accagctgcg tttcctggat
     5941 ctcgagttgg attttcgcaa tgtccttttc aagatcaact atgtccccct ggagagtttc
     6001 ttcctgttgt cctttggaag atatttcttt ctgatactcc tggatctgat tccgcagaat
     6061 gtttattttt gttttgagat cctgctgctg cgcatcattc tgggcagcca aagatacatc
     6121 ggatgccaaa aacaaaacaa ccaaacaaaa atatattgtt atttttgaaa tataactttt
     6181 cattttatat tctttgtatt tctacttttt attatacttt attccccaca aaaaaacacc
     6241 cgacgctctt gcatcgggtg ttttgaaata tttcagatta tttcttgtcc ttgcctgccg
     6301 aagaggcagg ttcttttttc tcctctggct tggcttcctc gccttcggcc ggagcctctt
     6361 ctttcaccac tccctcgact ttcgtgacat cctctttcac ttcggtttca agatcggcca
     6421 gttcttcttc cgagcgcggt tccgagacca tcgccaccac tgtttccgga tcgacaagta
     6481 cttcgacttt ttcagagaac ggcagatctt tcacatagat ataatcatca aaggttttga
     6541 tcggcgtgat gtccacttcc atttccggcg gcaaatctcc cggcaaacat ttcacctgaa
     6601 tcgcatccat atttttcacc agaatgccgc ccaactcttt cactgccgct gactcgcccg
     6661 tgaagaccaa ctcaatttcc gcttcgattt cttcatccat acgtacctga taaaaatcaa
     6721 catgccgcgc cgcgccgctc aaaacatcct tctggatatc ctttatcagc acattccgtt
     6781 cgtcttttcc atcaagcgcc agtttgaaaa ccgtgctctc tcccgcctct tcatatgtat
     6841 cattgaactt tttcttgtcg agagaaacca tcacattctc aatcccccgg ccatagagga
     6901 taccgggaat attttctttg tccgcaacct tgccgacgga tttcgttctg attttcgcat
     6961 caagtgtgat attttttgtc atctttagaa aatagcaatt accaatgaaa tctagcaaaa
     7021 aacaaatttt tcgtcaagcg gctagccgta tctttgtcgg ctgcctctgt acacgctttc
     7081 ggcaattcca agcatcgcgc atgtcgccac aagcgagctt ccaccatagc tcaaaagagg
     7141 gagtggaatt cccgccaccg gcgctatgcc gatattcatc ccgatattta cgataatttg
     7201 gatgaaaaac actattgcca cagcgccgac cagcaatttt ccaaaattgt cccggctttc
     7261 acgggcgatg gaaaaaagcc gcatcaatat gacaaaaagc aaaatcaaaa caaacacgac
     7321 tcccgtgaat ccaagttctt ccgcaatcac cgcaaaaata aagtcagtat gcttttccgg
     7381 aagaaaattg agctggcttt gcgatccatg tcccagccct ttcccccaca caccacccga
     7441 accaaccgcc acagtcgatt ggagaacatt gtatcccgca ccgtgaggat cactcgtcgg
     7501 actcacaagc accataattc tttgtttttg ataatctttc aaaacgaagt gccagctgaa
     7561 aatacccaaa agcaatccgg caatcaccag agcgatcaag tatctttttt tcactccgga
     7621 aagaagcaag actccggccc atgttccgac aatgaccaaa gcagatccca aatcgggctg
     7681 aagcatgatt aatgcaaccg aaagagcaac tggaacaagc gagatgaaaa tatgcctcgg
     7741 ttctcggatt gttttcacat tgagggaaaa atatttagca agaacaacgg caatgatcaa
     7801 tttgaaaatt tccaccggct gaaaattgac gacaccgaag ctgatccatc cggaagtccc
     7861 gttgacaatt tttccgaaaa ccaaaaccgc aaacaacaga aagatgccga cgagaaaaag
     7921 agctgacgac accgatcgga aaatacgata gtcggtaaac gaaaaagcaa gaaacaaaaa
     7981 aatgccgatg acagcgaaga tcgcctggcg aaaaaagtgg ctggtgtcat ttccgacaaa
     8041 attgtttcca gaatggctga tgggataaag aacagccaag ctcaatccca aaagcaagag
     8101 agcggccgca agcaaaatcc aatccatctt caaaatattt tttaccatct ttcttataat
     8161 tgcgtctcat cagcgcttat tgttgcccgc cgctgacatt ataggttcca tactgccccg
     8221 gaactccgtg ctgcttcata aagttgtcgc tttcaaaaac attcacttcc ggaacaatct
     8281 ctatttttgc cgtcaccgga tcaatcgcaa acgagctgtt ccagctgaca ttgatttctc
     8341 tttcctcgcc cgcacgaaca tcattgaaat tggtttggtt gatggcgatc ggagatccat
     8401 cccctcctct gatcacaacc gctgcggaaa tttcatggaa atcaaaaccg ctttgatttt
     8461 tcatttttgc cgtgagacgc gcaaatccgg attctcctcc gctagccaag ctgaactcct
     8521 tggcatagac cgtaatatcc ggttcttcaa attgggaaaa cttgctccat tcaaacgaac
     8581 ttatttcaaa atccaaactt tccggcttca catcagaggg tatattgaag gcaaggatat
     8641 atttcgtctg ccccggcaaa acaaaattag acccggcact tttggcgatt atttttcccg
     8701 ctccatcaag aagattgaag gaataatcaa acttggccga tccgaaaagc gagttcggat
     8761 tcacgatctt caccagcgcg tcatatttgc ccgtttctcc gggaacgatc gctttctcga
     8821 caactttgag gctttccgct tttggcattt ccgcgcaggc cgcgcaaggt ccgccacaat
     8881 ccactcccgt ttctccttgg ttttgaattt tgtccgtgca ggtcggcttc gtccggaaaa
     8941 gaaaatataa tcccgttcca accaagccaa aaatcacaat gtacgctaca acaatggtca
     9001 atcgctttat agtacggctt gaaggcgtca tattttgcaa ataatattta tgcccttata
     9061 ctatatcatt ttttttactt tgtcatctct catgatttga tttttaggcg gtttaattgt
     9121 actattcaag agtaaaaaat aagtaaaaaa taataaattt ttatgcaaaa gcaaccaaaa
     9181 attgaaattc tccgccattc cctctcccat gtcctggccg cggcggtctt cgagatgttt
     9241 cccgacgccc gctttggcat cgggccagcc attgaaaacg ggttctatta tgactttgat
     9301 ctcccgcgta cgctcattcc agaagatctt gagattctgg aggaaaaaat gcaggccatc
     9361 atcaaggcca atttcaaatt tgaaaaagcc agtctgtcga ttgccgaagc gttgaaagac
     9421 ttcaaaaaag ccaaacagtt gctgaagatt gaattgataa aagatattgc gaagaacgat
     9481 aaaaatataa aaaaagtttc catctttcgc accggaaaat ttgtcgatct ctgcagcggg
     9541 ccgcacatcg attcgaccgg agaaataagc ctgaaggctt tcaagctcac aaagatttcc
     9601 ggcgcctatt gaaaaggcga tgaaaaaaac aagatgctcc aacgcatcta tggcgtggca
     9661 tttgcaaccg acaaagaact ccggcaatat ctcgcgatga tgcaggaagc ggaaaaaaga
     9721 gaccaccgca agcttggcaa ggaattaggc ttgttcattt tctccgattt gattggtccg
     9781 ggacttccaa tttatacgga caaaggagca actatccgcc gagagatcat caattattcg
     9841 aatgaattgc aatcagcaat cggctatcag gaagtgcaca ctccgaacat aaacaaggcc
     9901 gaccttttca aagtttccgg ccactatgaa aaattcgaag gagatatgct aaaagttgtt
     9961 tctcattata ccgatgaaga atatttcttg aagcctcct
//
LOCUS       DATKGS010000149         5979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_26730, whole genome shotgun sequence.
ACCESSION   DATKGS010000149 DATKGS010000000
VERSION     DATKGS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26730"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VMQ48_03700"
     CDS             <1..800
                     /locus_tag="VMQ48_03700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUD09166.1"
                     /translation="IITSYKNPAVLRLCLESLKKNVLCENFEILVLDSSTEEDLEIMM
                     REDFSEIRFFPHKDNLGFLGLVNEGIENAEGNYYLILNDDIVIKNKSADILLEYLKNN
                     PTVGIVGPKLLNFDGKFQFSCFRFYTPLIILYRRTFLGKLKFAQKRINYFLYKDRDHD
                     KPMDVDWVMGSAMMTSRKAVGKVGLMDRTFFMYFEDVDWCRRFWENKYKVMYYPFAQM
                     FHYHRKGSASQGLLKAAFNRLTRNHIQSVMKYFWKYRGKPNPHVENF"
     gene            926..2182
                     /locus_tag="VMQ48_03705"
     CDS             926..2182
                     /locus_tag="VMQ48_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HUD09167.1"
                     /translation="MDIEKNEPKSKNFQKHFKVFSVVILVCVSFFAGTFYDKHLSSKS
                     ASSNTIEGLLAKGSSKDQPQNVDFSLFWEAWNLLDDKYVDTTKIDHQKMIYGAISGMV
                     KSIGDPFSSFMDPEESQQFSQELGGTFEGIGVEIGMKSNILTVISPLQGSPAEKAGLK
                     AGDKILKVGDKLTSDMSVDEAVSLIRGPKGTDISLTILHDKSNTPVEVKITRDQINVK
                     SVTLEWKDNNIAVIKISKFGDDTISGMNQIASQVVSQKAKGIILDLRNNPGGYLEAAV
                     EVSSKFIPAGKVVVSEEERGGGKKEYDARGGDILSGIPVTVLVNSGSASASEITAGAL
                     RDDLGTQLIGKKTFGKGSVQELEKLSGGSNLRVTIARWLTPNGDYIMEKGIDPTIDVD
                     LTDADYNSNRDPQMDKALEVIRGEIK"
     gene            2222..2668
                     /gene="rplI"
                     /locus_tag="VMQ48_03710"
     CDS             2222..2668
                     /gene="rplI"
                     /locus_tag="VMQ48_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HUD09168.1"
                     /translation="MKVVLLKDVEKLGREGEIKEVAEGYARNFLIPQNFVKPATDQAI
                     QEVELRKEKKTKSDQMELAEVQKIAEQLDGRELFIKVKEKDGTLFGSVNEKTIAKTFA
                     DEGLKIDPKNVKLEEPIKDVGDYDVKLELDHGLEANIKVILVPEET"
     gene            2749..4374
                     /locus_tag="VMQ48_03715"
     CDS             2749..4374
                     /locus_tag="VMQ48_03715"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HUD09169.1"
                     /translation="MKPKYIFVVGGVMSGVGKGIATSSIGNILQSKGFRVSAMKIDPY
                     LNIDAGTMNPTEHGEVFVLDSGMETDQDMGNYERFMNLTLPSENYMTNGLVYKSIIDR
                     ERAMGYGGRCVDVVPDVPKEIIRRIKKAQKIAKADIMLVEIGGTLGEYQGLLFLEAGR
                     MMRLENKGDVLFVLVSYLPIPSTVGEMKTKPTQYAVRSLNAAGIQPDIIIARSAVALD
                     KKRKEKLADHCNLREIDVISAPDIESVYEVPVNFEKDNMSGILLEKLGLKPKKKDMLK
                     WKAMVKNIKNSKKPLKIGIVGKYFGTGDFVLSDAYISVIEAVKHACYSFKRKPQIDWL
                     NAEEYEKNPAKVRELKKYAGILVPGGFGERGVEGKLKAIEFCRKNKIPYFGLCYGMQL
                     LVIEYARNVLKLKGANTTEINRNTKFPVIDIMPEQKKNLADKNYGATMRLGAYPAKLK
                     KNTVAIGAYKTPNISERHRHRWEVNPEYIQRLEKAGLVFSGTSPDGRLMEIAELPKKS
                     HPFHLGTQFHPEFKSSPLHPHPLFKEFIKSAIK"
     gene            complement(4434..4706)
                     /gene="rpmA"
                     /locus_tag="VMQ48_03720"
     CDS             complement(4434..4706)
                     /gene="rpmA"
                     /locus_tag="VMQ48_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HUD09170.1"
                     /translation="MAHRKAGGSTQLGRDSQSKRLGVKKYGGQKVEAGNIIVRQRGTK
                     FRPGENVKRGGDDTLFAMKPGAVKFASKKIVKFTGKLQRAKFVSVE"
     gene            4764..5213
                     /locus_tag="VMQ48_03725"
     CDS             4764..5213
                     /locus_tag="VMQ48_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09171.1"
                     /translation="MSILSRFKESHIGQFWLKHEQKIVLAVGIVLVAAISFEAGFLHG
                     QKNKQKSVVVNKAATAEAASASSFPTQAQTSNPNNSSANSKPASIENIATADNQKCAF
                     VASKNSNKYHLPTCRYAQNIKPENKVCFSSKEEAEGRGFQGAKCCIK"
     gene            complement(5243..5716)
                     /locus_tag="VMQ48_03730"
     CDS             complement(5243..5716)
                     /locus_tag="VMQ48_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HUD09172.1"
                     /translation="MKQRIRDYIKQFFLIDDTPHKVAGGAALGVFMGIMPGEGVLSTL
                     FLAYIFRLNRLAALAGVLAFNMWTTVLALPIAAAIGGLIFRIDPQKLSNDFHESLSLG
                     IQYLFSWNVLRETALPLLVGFIVLAGSIAVAIYFGLLYLLIKRKNIKKKEHLLSK"
     gene            complement(5713..>5979)
                     /locus_tag="VMQ48_03735"
     CDS             complement(5713..>5979)
                     /locus_tag="VMQ48_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUD09173.1"
                     /translation="SEYMDREKVDQVFSDLGRVLRTIRKTYKYDKDQLRLILEPLDKW
                     EAVLKVDGIALKNILGVLPAGARREAEKAKVVDKESKSFIVKKG"
BASE COUNT         1847 a         1314 c         1342 g         1476 t
ORIGIN      
        1 taataataac cagctacaaa aatccagccg tccttcggct ttgcctcgaa tcgctcaaaa
       61 aaaatgttct ttgtgaaaat tttgaaattt tggtcctcga cagctccaca gaggaagatt
      121 tggaaataat gatgcgcgaa gatttttcgg agattagatt ttttccccat aaggacaacc
      181 tcggattttt gggattagta aacgaaggaa ttgaaaatgc ggaagggaac tattatctta
      241 tcctgaatga cgatatcgta ataaaaaaca aatccgcgga tatcttgctc gaatatttga
      301 aaaataaccc gacggttgga attgttggtc cgaagttgtt gaattttgac ggaaaattcc
      361 aattttcctg cttccggttt tatactccat tgataatttt gtatcgaaga acatttttag
      421 ggaaactaaa atttgctcaa aaaagaataa attatttttt gtataaagac agagatcacg
      481 ataagccgat ggacgtggac tgggttatgg gatccgcaat gatgacttcg agaaaagctg
      541 ttggaaaagt tgggttgatg gacagaactt tctttatgta ctttgaagac gttgactggt
      601 gcagacggtt ttgggaaaat aagtataagg ttatgtatta tccctttgct caaatgtttc
      661 attatcatcg aaagggatcc gccagccaag gccttctaaa agctgccttc aatagactaa
      721 caagaaacca tatccagagt gtaatgaaat atttttggaa atatagaggg aagccaaacc
      781 cgcatgtcga aaatttctaa ttttcaattt ttaaacaatt acttaatttc tcaattcatc
      841 aatgtccaaa acttttgaaa attatatcat tggatcattg aaaattgttt gaaaatttga
      901 aattgaaaat tgaaaattca taatcatgga tatagaaaaa aacgagccaa agtctaaaaa
      961 ttttcaaaag cacttcaaag tattttctgt agtcatactt gtgtgtgtca gttttttcgc
     1021 gggcaccttt tatgacaagc atttatcttc aaaatctgcc agctcaaaca ccatcgaagg
     1081 tctgctggcg aaaggaagca gtaaagatca gcctcaaaac gtcgacttca gccttttctg
     1141 ggaagcctgg aacctgctcg atgacaagta tgtcgacacg acaaaaatcg accatcaaaa
     1201 aatgatctat ggcgcaattt ccggaatggt aaaatcgatc ggcgatccct tttcgagctt
     1261 tatggatcct gaagaaagcc agcaattctc gcaagaactc ggtggaacat ttgagggaat
     1321 cggcgttgag atcggaatga aaagcaatat tttgaccgtc atttctccgc tccagggctc
     1381 accagccgaa aaagccggcc tcaaagcagg cgataagatt ctgaaagttg gagataaact
     1441 cacatctgat atgtctgtcg acgaagccgt cagcctgatc cgcggaccaa aagggacgga
     1501 tatttctctc acgattctgc acgacaaaag caatacccca gttgaagtga aaatcacccg
     1561 cgaccagatc aacgtgaaaa gcgtcacgct ggagtggaaa gacaacaaca tcgctgtcat
     1621 caagatttcg aagtttggcg atgacacaat ttccgggatg aaccagatcg cgagccaagt
     1681 cgtatcgcaa aaagccaagg gcataatcct tgatctgcgc aacaatccgg gaggttatct
     1741 ggaagcagcg gttgaagtat cctcaaaatt catcccagct ggaaaagtgg ttgtttccga
     1801 agaagaacgc ggaggaggca agaaagaata tgatgctcgc ggaggtgata ttttgagcgg
     1861 catacccgtg accgtccttg tgaacagtgg aagcgccagt gcttccgaga ttaccgccgg
     1921 cgccttgcgc gatgatttgg gaacccagtt gatcggcaaa aaaacattcg gaaaaggatc
     1981 ggttcaggag ctcgaaaagc tttccggggg atcaaatttg cgcgtcacta ttgcccgctg
     2041 gctcacccca aatggcgatt atataatgga aaagggcatc gatcccacga ttgatgtcga
     2101 cctcactgac gccgactaca acagcaaccg cgacccacag atggacaagg ctcttgaggt
     2161 catccgggga gaaataaagt aggggataga cacaaaaata atattaaatt ataattagga
     2221 tatgaaagta gttttactca aagatgtcga aaaactcggc agggagggag aaatcaaaga
     2281 ggtagctgaa ggttatgccc ggaattttct gattccgcaa aattttgtca aacccgcgac
     2341 agatcaggct attcaggaag ttgaattgag gaaagaaaag aagacaaagt cagaccagat
     2401 ggagttggca gaggttcaaa aaattgccga gcagcttgac ggaagagagc tgtttatcaa
     2461 ggtcaaagaa aaagacggaa cgcttttcgg ctcggtcaat gaaaaaacga ttgccaaaac
     2521 ttttgcggac gaaggtctga aaattgatcc gaaaaacgtg aaactggaag aaccgatcaa
     2581 agatgttggc gactatgacg tgaaactgga gcttgatcac gggctggaag ccaatatcaa
     2641 agtgatcctg gtaccggaag aaacctagga tatcagattc ttaataattc aagtataggg
     2701 gtgatatttt ttcacctcta ttttctttaa ataaatctag aaaaaactat gaaaccaaaa
     2761 tacattttcg tcgtaggggg agtgatgagc ggtgttggaa aagggatcgc cacatcttcg
     2821 attggaaata ttctccaatc aaaaggtttt cgcgtttcgg caatgaaaat cgacccctat
     2881 ctcaacattg acgcgggaac aatgaatccg accgagcacg gggaagtttt cgttttggat
     2941 agcgggatgg aaacggacca ggatatggga aattacgaac ggtttatgaa cctgactctc
     3001 ccgtccgaaa attatatgac caacggcttg gtctataagt cgatcatcga ccgggaacgg
     3061 gccatgggat atggcggccg ctgcgtggat gtcgttccgg atgtcccgaa ggaaatcatc
     3121 cggagaataa agaaagccca aaaaatcgcc aaagccgaca ttatgcttgt ggaaatcgga
     3181 ggaacgctcg gtgaatacca aggcctcctt tttcttgaag ccggaagaat gatgcgcctt
     3241 gaaaacaaag gtgacgttct ttttgttctg gttagctatc ttcctattcc gtcaacggtc
     3301 ggagaaatga aaaccaagcc gacccaatac gcggtccgct ctctgaatgc cgccgggatt
     3361 cagccggata tcattatcgc tcgcagcgcc gttgctctcg acaaaaaaag gaaagaaaag
     3421 ttggccgacc attgcaacct tcgcgaaatt gacgtcatca gcgcaccgga tatcgaatcc
     3481 gtctatgaag ttccggtcaa tttcgaaaaa gacaatatga gcgggatctt gctggagaaa
     3541 ctaggcctca agccaaaaaa gaaagacatg ctgaagtgga aagccatggt aaaaaatata
     3601 aaaaactcca aaaaaccgct taagataggc attgtgggaa aatatttcgg aacgggagat
     3661 ttcgtcttgt ctgacgccta tatttccgtc attgaagccg tgaagcacgc ctgctattcc
     3721 ttcaagcgaa agccccaaat tgactggctg aacgccgagg aatatgaaaa aaatccggcc
     3781 aaagtccggg aactcaaaaa atacgcgggg attcttgtgc cgggcggatt tggagaacgg
     3841 ggagtggagg gaaagctcaa agccattgaa ttttgccgga aaaacaaaat tccgtatttc
     3901 gggctgtgtt atgggatgca gctcctagtc attgaatacg cgcggaatgt tctcaagttg
     3961 aaaggcgcaa atacgaccga aatcaaccgc aacacaaaat tcccggtgat cgacatcatg
     4021 cccgagcaaa aaaagaatct cgccgacaag aactatggcg cgacgatgcg cctgggcgcc
     4081 tatcccgcca aactcaagaa aaataccgtc gctatcggag catacaaaac gccgaacatc
     4141 tccgagcgcc accgccatcg ctgggaagtg aacccggaat atatccagcg cctcgaaaaa
     4201 gcgggactcg ttttttccgg cacctcgccc gacggccgcc tgatggaaat cgctgaactt
     4261 ccgaaaaaat cccacccttt ccatctcggc acccaattcc atccagaatt caagtcctcg
     4321 ccccttcatc cgcatcctct tttcaaggag tttattaaga gtgcaataaa ataatgcagc
     4381 aaaaaaatag aagcgctgtg cttctatttt ttcaagtatt ttttcagaca attttattcc
     4441 acgctgacaa atttagcgcg ttgcaatttt ccggtaaact taacaatttt ttttgaagcg
     4501 aatttgactg ctccgggttt cattgcaaaa agagtgtcat cgcctccgcg cttcacattc
     4561 tcgcccggac ggaattttgt tccgcgctgg cggacgatta tatttccagc ttccactttc
     4621 tgtccgccgt attttttgac accgaggcgt ttggactgcg aatctcggcc aagttgtgtg
     4681 gagccacctg cttttctatg tgccataaaa ttaacttaaa agtttcagaa tttactatag
     4741 aataatagat tattcgcgac aaaatgtcaa ttttatctcg tttcaaggaa tctcacatcg
     4801 gacaattttg gcttaagcat gagcaaaaaa tcgttttggc agtcggcata gtccttgtcg
     4861 ctgccatttc cttcgaagcg ggttttttgc atggacagaa gaacaaacaa aaatcagtgg
     4921 tcgtgaacaa agccgcaaca gccgaagctg cgagcgcaag ctctttccca acacaagctc
     4981 aaacaagcaa tccaaacaat tcttccgcca acagcaagcc ggcttcgatt gaaaatattg
     5041 caacggctga caaccagaaa tgcgcctttg tcgcgagcaa aaattccaac aaatatcatc
     5101 ttccgacctg ccgatacgcc caaaacataa aaccggaaaa caaagtgtgc ttttcttcca
     5161 aagaagaagc cgaaggcaga ggatttcagg gagcgaagtg ctgtatcaaa taatcccgta
     5221 gagccgaagc attgcctcgg ctctacttcg acaacaaatg ctctttcttt ttgatatttt
     5281 ttctttttat cagaagatag agaagtccga aataaattgc tactgcgata gatccggcca
     5341 ggacaataaa tccgaccaaa aggggaaggg cagtctcgcg aagaacattc cagctgaata
     5401 aatattggat tccaagactc aatgattcgt ggaaatcgtt cgagagcttc tgcgggtcga
     5461 tccggaagat caagccgcca attgcagccg cgatgggcag cgcgagaacc gttgtccaca
     5521 tattgaacgc cagcactccg gccagcgccg caaggcggtt caaccggaag atataagcca
     5581 aaaaaagcgt actcaagacg ccttctccag gcataattcc catgaaaacc cccagtgccg
     5641 ccccgccggc gaccttgtga ggagtatcat caatcaaaaa aaattgcttt atataatctc
     5701 ttattctttg tttcatcctt ttttgacgat aaagcttttc gattctttgt cgacaacttt
     5761 ggctttttca gcttctcttc gtgcaccggc aggcaggacg ccgagaatat ttttgagggc
     5821 gatgccatcc actttcaaaa ccgcttccca tttgtccaac ggctcaagaa tgagccgaag
     5881 ctgatctttg tcatatttat aggtttttcg gattgtgcgc agaacccgcc ccaaatccga
     5941 aaaaacctga tcaacttttt ccctgtccat atattcgct
//
LOCUS       DATKGS010000150         2917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U14557 k141_143526, whole genome shotgun sequence.
ACCESSION   DATKGS010000150 DATKGS010000000
VERSION     DATKGS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938067
            Sequence Read Archive: SRR8676595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 23:03:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 752
            CDSs (total)                      :: 713
            Genes (coding)                    :: 709
            CDSs (with protein)               :: 709
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143526"
                     /isolate="SMAG_U14557"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Sweden"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VMQ48_03740"
     CDS             complement(<1..371)
                     /locus_tag="VMQ48_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HUD09174.1"
                     /translation="MQVYEPSRLAYRKKIGTVKKCQFCDLLNIKSQICKSISGKYWQV
                     IANKYPYMDGNLMIIPKRHFKNLEDMNESEQKEFFETLIKTKKRLSKIFKTEDFNIGL
                     NIGERAGASIDHLHWQIIPRK"
     gene            complement(424..933)
                     /locus_tag="VMQ48_03745"
     CDS             complement(424..933)
                     /locus_tag="VMQ48_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HUD09175.1"
                     /translation="MKKAKDKVVADPCQLAGICDQLRNGGGKKVVLTTGAFDLLHEGH
                     LRYLEEARSYGDVLVVGINDDNFVKNLKGQERPIIDENARAFMIAGFGCVDYVHIFGD
                     RLEIVRAVRPNVFVMFAQSHCKPHEGDRPEQQHIVRENGGEVIILDEPMSRRYTTTSI
                     IKKLKGGAI"
     gene            complement(1021..1488)
                     /locus_tag="VMQ48_03750"
     CDS             complement(1021..1488)
                     /locus_tag="VMQ48_03750"
                     /inference="COORDINATES: protein motif:HMM:NF042809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HUD09176.1"
                     /translation="MSEECCKILSPAEWDQKEIIWRDKPFLKSHYFAFLHVPVNIGAK
                     ISEGMKKIEEAGLGAEQMVLSKNDGWFGADILIPIKTKTDAFEIELITGRFFTRLYDG
                     HYGDMRKWIRETRSWCRERGFEPQEYIFWYATCPKCSKKRGDKAQVVVFAKVE"
     gene            complement(1485..2156)
                     /locus_tag="VMQ48_03755"
     CDS             complement(1485..2156)
                     /locus_tag="VMQ48_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HUD09177.1"
                     /translation="MDKNLTDKQNMILETISEMIENGQGNPTAYRISKYLQKKGMKVE
                     SVKSVVQVIEALEKKELVKRDIFRKIFLVENENMPMGLEGVFQVPVYGLASAGDALAF
                     AEDNIDGYLQISESLLAPSAKAQLFAVKTLGDSMNKEKINDGDYVIFEKKDSDFEYDN
                     KIVVAVVNGLATIKRYKKLGNGVIGLFPNSTNTFHQPIYIHESDSFMIAGVFRKVLPV
                     KFVSL"
     gene            complement(2274..>2917)
                     /locus_tag="VMQ48_03760"
     CDS             complement(2274..>2917)
                     /locus_tag="VMQ48_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HUD09178.1"
                     /translation="SNAPRKKMFLDIETLPAPEHMHEVLKEIHAYKKKKKTRNGEKYS
                     TSFESFLAGTGLDGTFGRILCISYAINDDMIHCICDEDEKKVLENFWEAAKDADLFVG
                     FNIIEFDMKYLVQRSIVHGVKPRTKMRLSFAKYRSEPMYDVMHEWNQWSFGKGVGLDT
                     LARALGFGSSKTNMDGSEVFTYYKKGKIKEICAYCNADVEVTRKIYKRMNFEE"
BASE COUNT          709 a          726 c          542 g          940 t
ORIGIN      
        1 tcttttcgag gaattatttg ccaatgcaga tgatcaatcg aggcgccagc tctctcgccg
       61 atattcagac caatgttgaa atcttcagtt ttgaaaatct tcgagagcct tttctttgtt
      121 ttgattaatg tttcgaaaaa ttctttttgc tctgattcat tcatatcctc aagatttttg
      181 aaatgccttt tgggaataat catcaaattg ccatccatat agggatattt gttcgcgata
      241 acctgccagt attttccgga aatactttta caaatttgac tttttatatt caacaaatca
      301 caaaactggc actttttgac agtcccgatt ttttttctgt atgccaatct gcttggttcg
      361 tacacttgca taaaattaaa gaggctatgg ttttgtatat tatacccata gccccttgaa
      421 aaatcaaatt gcgcctcctt tcagcttttt gattattgat gtcgttgtgt atcttcttga
      481 catcggctcg tcaagaatta tcacttcgcc accgttctcc ctgacgatat gttgttgttc
      541 gggtcgatcg ccttcgtgag gcttgcaatg ggattgagca aacatcacga atacatttgg
      601 ccgaacagct ctgacgattt ccagccgatc cccaaatatg tgaacataat ccacgcaacc
      661 gaatccggca atcatgaaag ctcgggcatt ttcgtcgata atcggacgtt cttgcccctt
      721 gagatttttc acgaagttat catcgttaat tccaacaaca agaacatccc cgtagcttct
      781 cgcctcttca aggtagcgaa gatgtccttc gtgcaaaaga tcgaaagcgc ctgtagttag
      841 aacaactttt ttaccccccc catttcttaa ttgatcacaa atgccagcaa gctgacacgg
      901 atcggcgaca actttatctt ttgccttttt catattatca gcctcctgac tgaatatttt
      961 ggaatgaact gctttatttt gttttagcaa tttattttcc agtagtcaaa tttcactttt
     1021 tcattccacc tttgcaaaaa ccaccacttg cgctttgtcg cctctttttt tgctgcattt
     1081 gggacaagtg gcgtaccaga aaatatattc ttgaggttcg aagcctcttt cgcggcacca
     1141 gctgcgcgtt tcgcggatcc attttcgcat atcgccatag tggccgtcat atagacgcgt
     1201 gaaaaatctt cccgtgatga gttcaatctc aaaagcatcc gttttggttt tgatcgggat
     1261 caaaatatcc gcgccgaacc agccgtcgtt ttttgaaagc accatctgct ctgccccgag
     1321 tcccgcttct tcgatctttt tcattccctc ggaaattttt gcgccgatgt tcaccggcac
     1381 atgaaggaac gcaaaatagt gcgatttcaa aaagggcttg tcacgccaga taatttcttt
     1441 ttggtcccat tcagcgggag ataatatttt gcagcattct tcactcataa acttacaaat
     1501 ttcacgggca agactttgcg gaaaacgccc gcgatcatga atgaatccga ctcgtggata
     1561 tagatcggct gatgaaatgt gttggttgag ttcggaaaaa gcccgatcac gccattgccc
     1621 agttttttgt atcgcttgat cgtcgccaga ccattcacca ccgccacaac gattttgttg
     1681 tcatattcaa aatccgaatc ttttttttca aaaatcacat agtccccgtc gtttattttt
     1741 tccttgttca tcgaatcacc caaagtcttc accgcaaaaa gttgcgcttt ggcgcttggc
     1801 gcaagcaaac tttccgaaat ttgcagatag ccgtcaatgt tgtcttcggc aaacgccagc
     1861 gcgtcccccg ctgatgccag gccatagacc ggcacttgaa aaacaccttc gagtcccatt
     1921 ggcatatttt cgttttccac aagaaatatt ttgcgaaaga tgtcccgttt caccagctcc
     1981 tttttctcaa gcgcttcaat cacctgcacg acgcttttca cggactcgac tttcatcccc
     2041 tttttttgaa gatatttgct gatccgatac gccgtcgggt ttccctgtcc gttctcgatc
     2101 atttcgctga tggtttcgag gatcatattt tgcttgtcgg tgagattttt gtccatgttt
     2161 ttattttata gattatgtgg atagtatcac tctatcactc cgcaatatca ctgtcaagcg
     2221 caagttatcc acaatctaaa atccgagagg caatctcccg gatttaatct tgtctattcc
     2281 tcgaaattca tcctcttgta tattttcctc gtcacctcca catccgcgtt gcaataggcg
     2341 cagatttctt tgatcttgcc ttttttataa tatgtaaaga cttccgatcc gtccatattc
     2401 gttttcgatg atccgaagcc gagcgcgcga gcgagcgtat caagccccac tccctttccg
     2461 aagctccact ggttccattc gtgcatcacg tcgtacatcg gctcgctgcg atatttcgcg
     2521 aaagagagcc gcatctttgt ccgcggtttc acgccatgga caatcgagcg ctgcacgaga
     2581 tatttcatat caaactcaat gatattgaac ccgacaaaaa gatcagcgtc tttcgccgct
     2641 tcccaaaaat tttccaaaac ttttttctcg tcttcatcgc agatgcaatg gatcatatcg
     2701 tcattgatgg catatgaaat gcaaagaatc ctcccaaatg tcccgtcgag acccgtcccc
     2761 gccaaaaaac tttcaaaact ggttgaatat ttttcgccgt ttcgagtctt cttctttttc
     2821 ttgtatgcat ggatttcttt cagcacttcg tgcatatgct ccggcgcggg aagagtttca
     2881 atgtcgagaa acattttttt tctgggggcg ttgctat
//
