LOCUS       DATKOU010000001         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3464832, whole genome shotgun sequence.
ACCESSION   DATKOU010000001 DATKOU010000000
VERSION     DATKOU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464832"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VMU21_00005"
     CDS             <1..386
                     /locus_tag="VMU21_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUO75932.1"
                     /translation="NYWIGYFVGPKVFSKERSRFFNRDYLDRTHKFYERYGGKTIIIA
                     RFIPIIRTFAPFVAGIGRMTYLRFILYNIVGGISWIGTFLCGGYFFGGLPFIKQNFSL
                     VILAIIIISMLPGLMEFLRHKFSSR"
     gene            500..1129
                     /gene="tolQ"
                     /locus_tag="VMU21_00010"
     CDS             500..1129
                     /gene="tolQ"
                     /locus_tag="VMU21_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HUO75933.1"
                     /translation="MQESAIQLILHAGYVVKVVLLILLFFSVFSWAIIFYKHRYFSKA
                     SKESESFLRTFRAGRDMKGLYNTARGLQLSPSANVFKSVYADESRANKEEIKRLLRRY
                     EALEVAKLQSYLNFLATTGSTTPFIGLFGTVWGIMDAFRGIGTAGSASLAVVAPGIAE
                     ALITTAAGLAAAIPGVVAYNYYLSRAKILIIEMDDFSEELFDFFTKAAQ"
     gene            1126..1527
                     /locus_tag="VMU21_00015"
     CDS             1126..1527
                     /locus_tag="VMU21_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUO75934.1"
                     /translation="MRVERDREVMSEINVTPFVDVMLVLLIIFMVTAPLLQQGIDVNL
                     PKAKGKDMPPEERIALVIKRDQKIYMNDTPVTVGEMRRKLESISKMNPNVFLKADKDV
                     PYGLVAEVMGEIKEAGIEKLGMVTEPAIRMQ"
     gene            1629..>2137
                     /locus_tag="VMU21_00020"
     CDS             1629..>2137
                     /locus_tag="VMU21_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HUO75935.1"
                     /translation="MPSPYVVNLVGSEIRAEGNSTQAMNKVPEPPVPESPPKKENPAT
                     SPIREQKKPAKNEEQQVQEQIDALRAKRDVQKRANLRRDIISLKGTGAEGKAVRKAGT
                     LKGTTGKGEASVEADYYTKITKEIWQQWIFPETGDKHLEAIISIRIMRDGAVQISRVE
                     KSSGNSLFD"
BASE COUNT          573 a          518 c          545 g          501 t
ORIGIN      
        1 tgaactactg gatagggtac tttgtcggcc cgaaggtgtt cagcaaggag aggtcaagat
       61 tcttcaacag ggattacctg gaccgtaccc acaagtttta cgaacggtac gggggaaaga
      121 cgattatcat tgcgcggttc atacccatta tcaggacctt tgcgcccttt gttgcaggga
      181 tcggacgtat gacctacctg cgcttcattc tctacaacat cgtgggagga atttcatgga
      241 tcgggacatt tctgtgtgga gggtattttt tcggtggcct tccctttatc aagcagaatt
      301 tctccctcgt catccttgca ataatcatta tttcaatgct tcccggtctt atggaatttc
      361 tgcgccacaa attctcgtcg cgataacttt ctcccctttt tcccccacac atcaggagga
      421 gaacgcgagg gacaacgcat ttgacttttt gtgcctactt cttttaatct gaactaaaat
      481 gaattattaa ggggtgttca tgcaagaatc agctatccaa ctcatcctgc acgcagggta
      541 tgtggtaaaa gtagtattac tcatcctctt gttcttctcg gtcttctcat gggccatcat
      601 cttttacaaa caccgctatt tctcgaaggc gtcaaaggaa tctgaatctt tcctgaggac
      661 cttcagggcc ggccgtgaca tgaaaggcct ctataataca gccaggggct tgcagctcag
      721 ccccagtgcc aatgtcttta aatcggtcta tgctgacgag agccgcgcaa acaaggagga
      781 gataaagaga cttctcagaa gatacgaggc ccttgaggtt gcgaagctgc agtcatacct
      841 gaacttcctt gccactacgg gctccacaac gccgtttata gggctttttg gaactgtctg
      901 gggaatcatg gatgcgttca ggggaatcgg cactgccggt tccgcatccc ttgcagtggt
      961 agcacccggt atcgcagagg ccttgatcac cacggcagca ggactggctg cagcaatccc
     1021 cggggtggtt gcctacaact attacctcag ccgtgcgaag atccttatca tagaaatgga
     1081 cgacttctcc gaggaactct ttgacttctt tacaaaggcc gcccaatgag ggtggaaaga
     1141 gacagggagg tgatgtcaga gatcaacgtc accccttttg tggacgtgat gctcgtgctc
     1201 ctcatcatct ttatggtcac cgcgccactc ctccagcagg gcattgatgt caatcttccg
     1261 aaagccaagg gaaaggatat gccccctgaa gagcgaatcg ccctcgtaat aaaaagggac
     1321 cagaagatat acatgaatga cactcctgtg acagttggtg agatgaggag gaaactggag
     1381 tctatcagta aaatgaatcc caacgtgttc ttaaaggcgg acaaggacgt cccctacgga
     1441 ctggtggctg aggtcatggg ggaaataaag gaggcaggaa tagaaaaact cggaatggtg
     1501 accgaaccag cgatccgcat gcaatgaccg ggccctcttt gcaacgattc acccttcttt
     1561 ccctcgtctt tcacctcact tttttcctga cggcattttt gacagtgaga caatcagtac
     1621 accgcatcat gccttctccc tatgtggtga accttgtggg gtcagaaatc agggcagaag
     1681 ggaatagcac tcaagcgatg aataaagtgc cggaaccccc ggtcccagaa tcgccaccca
     1741 aaaaggagaa ccccgccact tcgcccataa gggagcagaa aaaaccggca aagaatgaag
     1801 aacagcaggt ccaggagcag attgacgcgt taagagcaaa aagagatgtg cagaagcgcg
     1861 caaacttgag gagggacatt atttctctca agggaaccgg cgccgagggg aaagctgtga
     1921 ggaaggcagg tactttgaag ggcacgacag gaaaaggaga agcctcggtg gaggccgact
     1981 actatacaaa gatcacaaag gaaatctggc agcaatggat attccctgag accggagata
     2041 agcatctcga agctatcata tccattagga taatgaggga tggtgctgtt cagatcagca
     2101 gggtagaaaa atcttcgggg aattccttgt tcgacaa
//
LOCUS       DATKOU010000002         4077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5004196, whole genome shotgun sequence.
ACCESSION   DATKOU010000002 DATKOU010000000
VERSION     DATKOU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5004196"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VMU21_00025"
     CDS             complement(<1..290)
                     /locus_tag="VMU21_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75936.1"
                     /translation="MGRVIIYFLLCVLLAVTGYLLLDSTEKEPLPPSSPVRQGTAEPG
                     KQMPQSLPEETQKLSLELMKPADIQEPQAAEESRLTTARDRELSLKAAEKLRA"
     gene            complement(278..676)
                     /locus_tag="VMU21_00030"
     CDS             complement(278..676)
                     /locus_tag="VMU21_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUO75937.1"
                     /translation="MSYLILRVMINALSIVIAVKLVDGITFSGEWWKMIIVGGVFGLV
                     NSFIKPLVQLFSIPFIILTLGLFTLIINTLMLGLTASLSDSLHLGFQIRGFWSAFWGA
                     IIVSIVSMLLSWVTGIKRIRYIRHSDKWEE"
     gene            727..1377
                     /locus_tag="VMU21_00035"
     CDS             727..1377
                     /locus_tag="VMU21_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75938.1"
                     /translation="MSTTASSFSVRKDFVLREDFLHNEYILWYPERKGGTMYMKSVML
                     RIMITFTASALLLCIGSCASAPYLITVPTDPREIEGTYTLILYGGRYSSDIENVAILD
                     KEGDQFTFMVYAPEFDYKVKKHISAKEALAEAEAFVRFHYAFWKAQLKGIVDPTGIPI
                     GYEIRPLYYPMDFGYPDVLDIDYILKKDNNVTVMISLKRELRRRFLDDESPFIFRP"
     gene            complement(1443..2093)
                     /gene="mug"
                     /locus_tag="VMU21_00040"
     CDS             complement(1443..2093)
                     /gene="mug"
                     /locus_tag="VMU21_00040"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HUO75939.1"
                     /translation="MAESPRYEGECKSKKQQIKKNRPTGMSSVRIWKPTNAQLLAAYG
                     KRVPDVIAPRLKVLFVGINPGLYSGAVSHHFARPGNRFWPVLHKAGFTDRQLSPFEER
                     ELLKHGYGITNIVNQSTAAASELSRAVLEKGGRALKAKVRRYRPRVVAVLGLEAFRIA
                     FGQPKACFGPQEERIGPSQLWVLPNPSGLNASYQLPALSRLFRKVRKAAECGKKTV"
     gene            complement(2167..2802)
                     /locus_tag="VMU21_00045"
     CDS             complement(2167..2802)
                     /locus_tag="VMU21_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75940.1"
                     /translation="MNNKNHDVIGLTQNIKNNLADFPALLHCWRFLVGVVYALNQYTK
                     IANSCPFTTLSDDEYVQETDNVLTSISKNTPPAENWLRGFFYNAALMRLDAAYERFFK
                     AYLDGKCNLNLECTACDKNKIDEPYLYAEIRKEFSSLFSEKKFEDSNFGKVRHEVNSL
                     KHYIGGANLTEREQPEILRQALDELIAFLKDPTVRKDLTMFSGGYPITGRK"
     gene            complement(2909..>4077)
                     /locus_tag="VMU21_00050"
     CDS             complement(2909..>4077)
                     /locus_tag="VMU21_00050"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HUO75941.1"
                     /translation="ELELLSMPRRSFVVHFPVRMDSGKTRMFVGYRVQYNDARGPTKG
                     GIRFHPELTIDHVRDLAFLMVLKCAVVNIPFGGSKGGVMVNPKSLSKDELEQVTRGYI
                     RAIANFIGPFKDIPAPDVYTDEQIMVWILDEYERIRGEHVPSIVTGKPLELAGSRARR
                     YSTSLGGIYILEEAMKKRAMVHSNTRVVVQGFGHVGENAARILYEQGYRVIAVSDSKG
                     GIINEKGLDVHEVIRHKEGTGGVENFKGSANITNAELLTSSCDILIPAALSDQLHRDN
                     APHVRAKIILELANAPSTTDADDVFEEKQIMVVPDILANAGGVVVSYFEWSQNLNSDY
                     WEEERVLQKLKNTMITAFNDVHGMCREESCRLRRAAYRLAVKRVLHAERLRGNV"
BASE COUNT          965 a         1061 c          981 g         1070 t
ORIGIN      
        1 gccctcaact tttccgcagc ttttaacgag agttctctgt cccttgctgt ggtcaggcgg
       61 gattcctctg cagcctgcgg ctcttgtatg tcagcaggct tcattaattc aagagacagc
      121 ttctgagtct cctcgggcaa agactgtggc atttgctttc cgggttcagc tgttccttgc
      181 ctaacgggag aagaaggggg aagaggctct ttttctgtgc tgtcgaggag gagataccca
      241 gtgactgcga gcaggacaca caacagaaag tagataatta ctcttcccat ttatcggaat
      301 gtctgatgta tctgattcgc tttatgccgg tgacccatga gaggagcata ctcacgatgc
      361 ttacgattat cgctccccaa aatgcagacc agaagcctct tatctgaaac ccgaggtgga
      421 gggagtctga aagggaagcg gtcagtccga gcatgagggt gttgatgatg agcgtaaaga
      481 gcccaagggt caggatgata aagggaatgg agaagagctg tacgagcggt tttatgaagg
      541 aatttacaag gccgaagacc cctcccacga taatcatctt ccaccattct ccggaaaagg
      601 tgatcccgtc cacgagtttg accgcgatca caatggagag ggcattgatc attaccctca
      661 ggatgagata ggacatataa ctccaatcac gtatttcata gttcatacta gcaatcaggc
      721 caatttatgt caacgactgc ctcctcattc agtgtgcgca aagactttgt cttaagggaa
      781 gactttcttc acaatgagta tatcttatgg taccctgaaa ggaagggtgg gactatgtac
      841 atgaaaagtg tgatgctgcg cattatgatc actttcaccg cttctgcact tctcctgtgc
      901 atcggttcct gtgcctcggc gccctatttg atcactgtac caaccgaccc ccgcgagatc
      961 gaagggacct atacgctcat cctctacggt gggagataca gcagtgatat tgagaacgta
     1021 gcgatactgg ataaagaggg ggaccagttt accttcatgg tgtatgcccc tgaatttgat
     1081 tacaaagtga agaagcacat ctccgccaaa gaggcgctcg cagaggccga ggcatttgtt
     1141 cgtttccact acgcattctg gaaggcccaa ttgaagggga ttgtggaccc gaccggtatt
     1201 ccgattggat atgagattag acctctctat taccccatgg attttggcta tcctgatgta
     1261 ctagacattg actacattct taagaaagac aacaacgtca cggtgatgat atcactgaag
     1321 cgggagctga ggagacgttt ccttgatgat gagagcccct tcattttcag accgtgaaga
     1381 tgaagagaag gcaagtacag gcacaaaaag tcaggacttt gccgatgcac ggctgatcct
     1441 gatcatactg ttttcttccc gcactctgcc gcctttcgga ccttccggaa caacctgctg
     1501 agggcgggga gctggtagct tgcattcagg ccgctggggt ttggcaggac ccagagctgc
     1561 gaagggccga tgcgctcctc ctgggggcca aagcaggctt tcggctgtcc gaaggcgatg
     1621 cggaaggcct cgaggccgag cactgcgact acgcggggac ggtagcgccg gaccttcgcc
     1681 ttcagtgcgc ggcctccctt ttccaacacc gcccggctaa gctccgacgc tgcagccgtc
     1741 gattggttca cgatgttcgt gatgccatac ccgtgcttca gcagttcccg ttcctcgaac
     1801 ggtgagagct gccgatccgt gaacccagcc ttatgcagaa cgggccagaa ccggtttccc
     1861 ggccttgcga aatggtggct cacagcgccg gaatagagac ctgggttgat ccccacgaaa
     1921 agtaccttta agcgtggcgc gatcacgtca gggacgcgtt ttccgtacgc agcaaggagc
     1981 tgggcgtttg tcggtttcca gattctgact gatgacattc ctgttggtcg gttctttttg
     2041 atttgttgct ttttgctttt gcattcaccc tcataacgag ggctttcagc caccagcgca
     2101 acttctccgc tggaaaatca aaccagcgtt attcctggtc ggtacgagtg atttgttagg
     2161 caactttcat ttcctgccgg taatgggata acccccagaa aacatggtta gatcctttct
     2221 tactgtcgga tccttaagga aggctattag ttcgtccaga gcttgtcgaa gtatttcagg
     2281 ttgttctctt tccgttaggt tagccccccc gatataatgt tttagactat taacctcatg
     2341 acgtaccttc ccgaaatttg agtcctcaaa tttcttttcc gaaaataatg agctaaattc
     2401 ttttcttatt tccgcataca gataaggttc atctattttg tttttgtcac acgcggtaca
     2461 ttcaagattc aaattgcatt tgccatcaag gtaagcttta aaaaagcgtt cataagccgc
     2521 atccaatcgc attagtgcag cattgtaaaa aaaacctctt agccaattct ctgcaggcgg
     2581 cgtgtttttt gaaatggaag tgaggacgtt atccgtctcc tgaacatatt cgtcatcaga
     2641 aagcgttgta aatgggcagg aattagctat tttggtatac tggttaaggg cataaacgac
     2701 gccaacaagg aatctccagc agtgaagtag cgccgggaaa tctgcaagat tgttcttaat
     2761 attttgcgtt aaacctatta catcatggtt cttattattc acttatatat gccgcttaac
     2821 gacgagttca cacgccgcga agcggtcggc gtggaaccct ttctgagctt caaaagttcc
     2881 ttttgaagct gagtgtcacg aagccctgtc acacattccc gcgcagcctc tccgcgtgga
     2941 gcaccctctt caccgcaaga cgatatgccg cccttctcaa ccggcagctc tcctcgcggc
     3001 acattccatg cacgtcgttg aatgcagtga tcatggtgtt tttcagcttc tgcaaaaccc
     3061 tctcttcttc ccaataatcg ctgttcaggt tctggctcca ttcaaagtaa ctcaccacga
     3121 caccgcccgc attggcgagg atatccggca ccaccataat ctgtttttcc tcaaacacat
     3181 catccgcgtc cgtggtggag ggggcattgg caagctccag aataatcttt gcccgcacgt
     3241 gaggcgcatt gtccctatgc agttggtctg acaatgcagc agggatcaaa atgtcacaag
     3301 aggaagtgag caactctgca ttggtaatat tggcactgcc cttgaaattc tccacgcctc
     3361 cggtaccctc tttatgtctt atcacctcat ggacgtcaag ccccttctca ttgatgatgc
     3421 ctccctttga atcactcact gcgatgaccc tgtatccctg ttcataaagg atgcgggcag
     3481 cgttctcgcc cacatgcccg aatccctgga caactaccct tgtattggag tgcaccattg
     3541 ccctcttttt catggcctct tcaaggatgt aaatccctcc cagagaggtg gaatagcgtc
     3601 ttgccctgct tcctgcaagc tcaaggggct ttcctgtcac tatggacggg acatgctctc
     3661 ccctgatccg ttcatactca tcaagtatcc agaccataat ctgctcatcg gtatatacgt
     3721 cgggtgcggg gatgtccttg aagggaccga tgaaattcgc aatggcacgg atatatcccc
     3781 gggttacctg ttcgagctcg tccttgctga gggacttggg attcaccatc acgcctccct
     3841 ttgaacctcc aaagggaatg ttcaccaccg cgcacttgag aaccatgaga aatgcaaggt
     3901 ccctcacgtg gtcaatggta agttcaggat gaaaccttat tcctcccttg gtgggacctc
     3961 tcgcgtcatt gtactgaacc ctgtacccca caaacatcct tgttttccct gagtccattc
     4021 tcacaggaaa atggacgaca aacgatcgcc ttggcatgga cagcagctcc agttctt
//
LOCUS       DATKOU010000003         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4623345, whole genome shotgun sequence.
ACCESSION   DATKOU010000003 DATKOU010000000
VERSION     DATKOU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4623345"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..875)
                     /locus_tag="VMU21_00055"
     CDS             complement(3..875)
                     /locus_tag="VMU21_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUO75942.1"
                     /translation="MPCRVLGIFIITLFAIVSCAHREIKPPPKQAKVALVLGAGASRG
                     FAHIGVLKVLEANKIPVHMIIGTSAGSFVGSMYAYGYNSFQLQKMSFSMEKGDIIDLV
                     VPDNGFVKGEKLEEFINKTVNNTPIEKMRIPFYAIAADIQNGQEMVFGKGNTGKAVRA
                     SCAIPGIFRPVKIENRIYVDGGVVSPVAVDAAKRLGADVVIAVDVTSAFENIQPEGTI
                     DTILQSIGIMYAKLAAQQIVRADVVIRPKVGSIAAGDFSKRHEAVLEGEKAALEALPA
                     IQSIIAGLKQEGRL"
     gene            complement(975..1904)
                     /locus_tag="VMU21_00060"
     CDS             complement(975..1904)
                     /locus_tag="VMU21_00060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUO75943.1"
                     /translation="MVKARILLVEDSRTQAEVTKDFLERNGYEVDWAEDGKSAIKKVK
                     TQAVDIVLLDFVLPDINGNEVCRWLKLDGDTRGIPIIMLTTRSDTSDKVSGLEAGADD
                     YLPKPYSEMELNARIYASLRTKALQDELRQKNRQLEEMLGKVETLAITDPLTGLSNRR
                     HCEDTLENEFKRAVRYQSSLSCLMLDIDHFKRVNDVYGHQAGDMVLKEAAQILKSNIR
                     ELDTAARWGGEEFIVLLPQTTKQDAMVLASRFLSAISDHIFSGIPNEHISASIGVAGI
                     PDPSIDTGEKLLDAADRALYEAKSKGRNRVASI"
     gene            complement(1908..2171)
                     /locus_tag="VMU21_00065"
     CDS             complement(1908..2171)
                     /locus_tag="VMU21_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75944.1"
                     /translation="MREHRKKLERGGFVRQVLIMLTLAVVFTISGCSGSGGKELFETA
                     KFEELQNNPQHATELYEEIVKKYPKSEYAKKAEERLNVLKGNK"
     gene            complement(2215..2502)
                     /locus_tag="VMU21_00070"
     CDS             complement(2215..2502)
                     /locus_tag="VMU21_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75945.1"
                     /translation="MTVICKCCKYGLEMSPDLRRPPAGTVWCSKRKVPMGQNRNMSCF
                     VPLVVERGRRCQECKWAKMLKPSGEAPLLGKIWCEKRHFEVNKLRTMECFE"
     gene            2855..>3065
                     /locus_tag="VMU21_00075"
     CDS             2855..>3065
                     /locus_tag="VMU21_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HUO75946.1"
                     /translation="MNFSEEELKRYHRQMLMDGWGEETQKKLKNSTVFVAGAGGLGSP
                     VSIYLAVAGIGHIRICDFDSPDWSNL"
BASE COUNT          709 a          871 c          653 g          832 t
ORIGIN      
        1 acttacagtc ttccttcctg cttaagcccg gcgatgatgg actggatggc gggcaacgcc
       61 tcaagggcag ccttctctcc ttcaaggacc gcctcatgcc tttttgaaaa atcccctgct
      121 gcgatagaac cgaccttcgg ccttatcacc acatctgccc tcacaatctg ctgtgcggcg
      181 agtttggcat acataatacc tatggattgg agtatcgtat cgatggtacc ttcaggctgt
      241 atgttctcaa acgcggaagt gacgtccacc gcgatcacca catctgcccc gagcctcttc
      301 gcagcatcca ctgcaacagg actcaccact cccccatcaa catatatcct gttctcgatc
      361 ttcactggcc tgaatatccc tgggattgcg cagctcgccc tcaccgcctt ccctgtgttg
      421 ccttttccaa agaccatttc ctgaccattc tgtatgtccg ccgcaatagc ataaaaggga
      481 atacgcattt tctctatggg cgtgttattc acagtcttat tgatgaactc ctcaagcttt
      541 tcccccttaa cgaatccgtt gtcaggaacg acgagatcaa taatatctcc tttttccatg
      601 gaaaacgaca tcttctggag ctgaaaggag ttgtatccat aggcgtacat gcttcccaca
      661 aaactcccgg cgctggttcc gatgatcatg tgcacaggta tcttgttggc ctccagtacc
      721 ttgagcacac cgatgtgggc aaatcctctt gatgcgcctg ccccgagcac cagtgccacc
      781 ttcgcctgtt tcggcggagg ttttatctca cggtgagcgc atgaaactat ggcgaacagt
      841 gtaatgatga aaatgccgag aaccctgcat ggcatactcc catagaatag cacaaacgtc
      901 tccccggaat gcttctcaag aagggtctca acctcccgca cgtcgcgtaa gggatggggg
      961 ccgaaagaaa taccctatat gctcgccacc ctgttacgtc ctttactttt cgcctcatac
     1021 aaggcacggt cggctgcatc cagcagtttt tcccctgtat cgatggaagg atcgggtatg
     1081 ccggcaaccc cgatactcgc gcttatatgc tcattgggaa taccagagaa tatgtggtca
     1141 gaaattgcac tcaaaaacct cgatgcaagg accatggcat cctgctttgt cgtttgggga
     1201 agtaatacaa taaactcctc tcctccccat cgcgccgcag tatcaagctc ccttatgttg
     1261 ctcttaagga tttgtgctgc ctctttgagg accatgtctc ctgcttgatg gccatagacg
     1321 tcattcactc tcttaaaatg gtcgatatca agcatgagac atgagagtga agattggtac
     1381 cgtactgccc tcttgaattc attttcgagg gtatcctcgc aatgccgcct gttggaaagc
     1441 cctgttaacg ggtccgtaat cgcaagtgtt tccacctttc cgagcatttc ctccagctgg
     1501 cgattcttct gtcttaactc gtcctgcagc gccttcgtcc ttaaagacgc atatatcctt
     1561 gcattgagtt ccatctcgct gtaaggcttg gggagatagt cgtcagcacc tgcctccaag
     1621 cccgaaacct tgtcgcttgt atcacttcgt gtggtgagca tgatgatggg tatacccctg
     1681 gtatccccgt caagcttcag ccatctgcat acctcgttac cattgatatc ggggaggacg
     1741 aaatcgagga gcacaatatc cactgcttgg gttttgacct tcttgattgc agacttaccg
     1801 tcttcggccc agtcaacctc atacccgttt ctctccagaa aatcttttgt aacctctgcc
     1861 tgggtcctgc tgtcttccac aagaagaatt cttgccttca ccattttcta tttattccct
     1921 ttcagaacat tcaatctctc ttccgctttt tttgcatatt cgcttttcgg gtacttcttc
     1981 actatctctt catacaattc cgttgcgtgc tgtgggttgt tttgcaactc ttcaaatttt
     2041 gcggtctcga agagttcctt gccgccactc cccgaacacc cggaaatcgt aaagacaacg
     2101 gccagtgtga gcatgataag cacctgtctc acgaatcctc ctcgttccaa cttttttctg
     2161 tgttctctca taaaaccctt ctgcccacgc ctctgcagca ccttcctttg ccctctattc
     2221 aaagcattcc atggtcctga gtttattcac ctcgaaatgc ctcttctcac accatatctt
     2281 acccagcaac ggcgcctcac cggaaggctt caacatcttg gcccacttgc actcctgaca
     2341 tctcctgccc ctctccacca ccaaagggac aaagcaggac atatttctgt tctgacccat
     2401 ggggaccttt cttttgctgc accagacagt gcccgcagga ggcctgcgca ggtccgggga
     2461 catctcaagg ccgtatttgc agcacttaca gattaccgtc atggtaattt tgtcgatgaa
     2521 tcaagaaagg cactcctttc cttgcatgag atattttcac actcccctgc gctctgcggg
     2581 agagagcaga ccttggaggc cgcgtatgcc gagaagggat tccagaagaa tatctgatgg
     2641 ttaccatttt ccacatgagt caaaagtcgt cttagtctat accacaaaat acacctttgt
     2701 aaataatcca tacgcgcata aaaagcctgc attcgctgtt tcgtaccacc ctacagctat
     2761 catgtccctc aaagttgata aaaaggacga gcggagcata ttatagaacg catatcttat
     2821 ttgcagggtt cagtatcccg atccggagga aaaaatgaat ttttcagagg aagagctgaa
     2881 acgatatcac cgccagatgc ttatggatgg ctggggtgag gaaacacaaa agaaactcaa
     2941 gaactccacg gtatttgtgg cgggcgcagg ggggctcggc tcgccggtgt ccatatacct
     3001 cgcggtcgcc ggaatagggc atataaggat ctgtgacttt gattcccctg actggtcaaa
     3061 cctta
//
LOCUS       DATKOU010000004         3055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2315098, whole genome shotgun sequence.
ACCESSION   DATKOU010000004 DATKOU010000000
VERSION     DATKOU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315098"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..979
                     /locus_tag="VMU21_00080"
     CDS             407..979
                     /locus_tag="VMU21_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75947.1"
                     /translation="MMSEKERPLPKPPTTPFGRKRAFEERDENVPLMADRMVAAMAEG
                     RLDEFMKQELPDSEQARTLAMMMMGMSGMMPPQGLPSVQKNEPQGESGNPPSEPPDEV
                     VKAAKEGDVKGLMEILQREHKQRSADTVTEEDIAPENADETSPSVGKAEKETLDQLMD
                     IASRNNLSMDWVVLRALRLYVEEYHKTGRL"
     gene            complement(1058..1969)
                     /locus_tag="VMU21_00085"
     CDS             complement(1058..1969)
                     /locus_tag="VMU21_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUO75948.1"
                     /translation="MGPTDLIDGYRRSLKRRNCSVHTVRNYMNILDRFHAWLQVPLEQ
                     ATSKESDAYMDYLLRERKKPKTINCHFSCIHAIYKYLIEDERMALTNPVKKHYRLRLP
                     RPLPKHLRDEQVAALFGEISDTRDRAMFMLMLRCGLRVEEVARLTADAIEYRSRQLYV
                     FNGKGSKDRVVYLSEDALSALASYAEKRKWSKEKKVFLVQKGPLKGKPISVRGIQKRI
                     EHYARRAGIQASCHHLRHTMATQLLNADADLVTIQDLLGHSRITTTQCYCRVSNLKVQ
                     RDYFKAIMVVLQRTQPIRGNEYADLTG"
     gene            complement(1956..2963)
                     /locus_tag="VMU21_00090"
     CDS             complement(1956..2963)
                     /locus_tag="VMU21_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUO75949.1"
                     /translation="MTRAGAILRAIVSDSEAANYQNVVETPIDRILRKISRLDLSGGE
                     HFERYMLHKWRMNHKPSTLKGSFVALSSFLIFYEGTGKSQIEEIVSNDLEAFIEHEQD
                     RELKVTTVRTRLHCLFAFLRFLIEQDIIDGRILKRKITLKVPDFLPRAIAPGDIRKIL
                     SVIKEVRDRALILVLLRTGMRIGEVLRLNIRDLDVRERKIHIYEGEKNCLGRVVYLSD
                     DAVMALGLWLKKKEKGKAYLFPGLKGNLCYTSARNIFVKYLREAGLQQKDYTIHSLRH
                     TFASELLNAGMRLECLQLLLGHRDIEMTRRYARLTDKSRQEEYFRAMEIIEQGGIHGT
                     Y"
BASE COUNT          771 a          798 c          704 g          782 t
ORIGIN      
        1 ttccctcatc aacaccattc atcccgaaaa agaccactac gaagacggaa tcagcaacac
       61 cctcccctaa ctagtacgta tatctgttcg ctcaagtcct aacccggatt attcataaga
      121 gtgaaactta agaggcttcg ccgggtctga ggcaaaatac ttggagttat tggtgcggcc
      181 ggatatgagg ccgcgtaaat tcaacaattc acggaccgca attaggtgaa cttgatggat
      241 agagagacac ggagggctat tgtatcttgc cttggggcgg gcgagcctct gaccatgaag
      301 aaacttatga attaagtggc ctacgttcgg catttgcttt cccattgcca cgattttccc
      361 tgtttcatct atggtatagt agaaagaaaa cgcatccagg gaaggaatga tgtcagaaaa
      421 agagcgccct ttacccaaac cacccaccac accatttgga agaaaaagag cttttgaaga
      481 aagggatgag aacgttcccc tcatggccga ccgaatggta gcggccatgg ctgaaggaag
      541 gctggatgaa ttcatgaaac aggagctgcc tgatagcgaa caagccagga cccttgccat
      601 gatgatgatg gggatgagcg gaatgatgcc tccccaagga ctcccttccg tgcagaagaa
      661 tgagccccag ggagagtccg ggaaccctcc atcagagcct cctgatgagg tggtaaaggc
      721 agcaaaagaa ggggacgtga aagggctcat ggagatcctc cagcgggagc ataagcagag
      781 gtcagccgat accgtcacag aggaagatat tgccccggaa aatgcagacg agacctctcc
      841 ttccgtcggt aaggcggaga aagagaccct ggaccagctc atggacattg cgtccagaaa
      901 caacctctcc atggactggg tagtcctgag ggcactcaga ctctatgtgg aggaatacca
      961 caagacaggc cggctctaag aatttcccta cgtcaacaag cccgttctga gcagttcacc
     1021 atgctatacg taacataacc tgctttttct tttattttca gccggttaaa tctgcgtatt
     1081 cattacccct tattggctgg gtcctctgca atacaaccat tatggcctta aagtaatctc
     1141 tctgtacctt gagattggag actctgcaat agcactgggt tgtagtaatg cggctgtggc
     1201 ccaacagatc ctgtatggta acaagatcag catctgcatt gagcagttgg gttgccatag
     1261 tgtgcctcag gtgatgacag gatgcctgta tcccggctct tcgcgcataa tgttcaatcc
     1321 tcttttgaat gccgcgtact gatattggct ttcccttgag tggtcccttc tgtaccaaga
     1381 acaccttttt ctccttggac catttccgtt tctccgcata cgacgccaag gcactcagag
     1441 catcctcgct gagatagaca accctgtcct tactgccttt tccattgaag acataaagct
     1501 gtctgctgcg atactctatc gcatctgctg tcagccgtgc aacctcttca acacgcagac
     1561 cgcacctgag cataagcatg aacatggccc gatcccgcgt atcgctgatc tcgccaaaga
     1621 gtgccgccac ttgctcgtct ctgagatgtt tgggcagcgg tctgggaaga cgcagcctat
     1681 aatgcttttt caccggattg gtgagcgcca ttctctcgtc ttcgatcaga tacttataga
     1741 tggcatggat gcagctgaag tggcagttaa tcgtcttagg tttcttcctt tccctcaata
     1801 ggtagtccat atacgcatcg ctttctttgg aggttgcctg ctctaaagga acctgcagcc
     1861 aggcatggaa ccgatcaagg atattcatgt agtttcttac agtgtggacc gagcagtttc
     1921 ttcgtttgag agatcgtcga tatccgtcaa tcagatcagt aggtcccatg aatgcctccc
     1981 tgctcgatga tttccatggc acggaagtac tcctcttggc ggctcttatc ggtcaggcga
     2041 gcatatcgcc gggtcatctc tatatcgcga tggcccaaca gcaactgtag gcactccaaa
     2101 cgcatcccgg cattgagcag ttcacttgca aaggtatgcc gaagactgtg gatcgtatag
     2161 tccttctgct gaagccctgc ttccctaagg tacttcacaa agatattgcg ggccgaggtg
     2221 tagcacaaat tgccctttaa cccggggaaa agatatgcct ttcccttttc tttcttctta
     2281 agccagagcc caagggccat cactgcatca tcactcagat aaaccacccg accaagacag
     2341 ttcttttcac cctcatagat atggatcttc ctctcccgta catctaggtc cctgatattc
     2401 aagcgaagca cttccccaat tctcatgcct gtccttaaga gtacgagtat cagggcacgg
     2461 tcacgaacct ctttaatgac actaagaatc tttctgatat cccctggtgc aatggccctg
     2521 ggcagaaaat ccggcacttt gagtgttatc ttgcgcttga gtatacgtcc gtcaatgata
     2581 tcctgttcga ttagaaagcg aaggaaagca aaaaggcaat gaagcctcgt cctgaccgtt
     2641 gtgactttga gctcccgatc ttgctcatgt tcgataaatg cctctagatc gttactgaca
     2701 atctcctcga tctgactctt ccctgtgcct tcatagaaaa ttaggaagga actcaaagca
     2761 acaaagctcc ccttaagagt gctcggcttg tgattcattc tccacttgtg gagcatgtaa
     2821 cgctcgaaat gctctccgcc ggaaaggtcc aacctggaga tcttccgaag aatacggtca
     2881 attggggtct ctactacatt ctgataattt gctgcttcac tgtcactgac gattgctctg
     2941 agaattgcac ctgctctggt cataaatcct cctttttctc cttgaaaaag caggccttat
     3001 gatgaccttc agcggtctca cgattcaacc cattttgtta cgtatagcac cgact
//
LOCUS       DATKOU010000005         7384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5010854, whole genome shotgun sequence.
ACCESSION   DATKOU010000005 DATKOU010000000
VERSION     DATKOU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5010854"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VMU21_00095"
     CDS             <1..1095
                     /locus_tag="VMU21_00095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HUO75950.1"
                     /translation="KEKKTLDPFVHYAVAAAAMAAEDAGIPTYAPLTEGGVRGENHSF
                     LNSAGVIIGSSRGGISTIEKALISQHSLSRSNTRRYRLSPYLMPSTTIGMAASYVAQK
                     LGIIGYCLGISNACSSGTNAIGEAFRLIKSGYAHLVLAGGSEAPLCRLCVEGYGIAGA
                     LSKQKRHSGVGPSTPRPFDKARDGFVLAEGSCILVLEDYESALERGAPLYGEIIGYGN
                     TTDAFHITRPSPKGEAKAMIKAIEEAGLRMDDVDYINTHGTGTQIGDMAEAQAIKMVF
                     GEKTTLIPATAIKSLTGHMLAASGAFEIASTLMSMREGIIPPTINLDAKDPECDINVI
                     TEMTPADIRVAISNSFGFGGVTAVIVLRKI"
     gene            complement(1249..2352)
                     /locus_tag="VMU21_00100"
     CDS             complement(1249..2352)
                     /locus_tag="VMU21_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75951.1"
                     /translation="MKALEVRSERDLADFINFPYSLYAQDPLYSPPLKREMKEQFSIK
                     NPFFQHAEVRYFLARKKGKTLGRVVSIINRRHIDFHNERVGFFGFFESVNAREVSHCL
                     LDAVSDALRGKGMAIMRGPMNFSTNEECGFLVEGFHNVPMLMTPHNPPYYNNMMEQYG
                     MQKAKDLYAFLYAVKESLPEKVLRVAAIAEKRGIQVRPVDKKKFNEEMRVFQKVYNSA
                     WEKNWGFIPLTDEELQYLGERLKQIAVPELTLIAEDDGEPVGFMGLLPDFNYVLRHMK
                     GRLNPVSILKALYYSKKITDLRLLLLGIKKEYRNRGVDALLFREGFKGVKSGGYRRVE
                     FSWILEDNIPVQRIVEMIGGRLYKRYRVYEKPI"
     gene            complement(2358..3587)
                     /locus_tag="VMU21_00105"
     CDS             complement(2358..3587)
                     /locus_tag="VMU21_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HUO75952.1"
                     /translation="MDSRLVQDPSMAVKKTIDIFEKCGKFTKAKELMSAGLYSYFRVI
                     ESAQDPEVMMHGKRLIMVGSNNYLGLTNHPKVKEAAIDAIRKYGSGCAGSRFLNGTLN
                     IHVELEKKLARFIRKEVAVVFSTGFQVNLGVISALVGKDDIVIIDKMDHASIIDGCRL
                     SYGEVKKFKHNDMDNLERILDQNNGRGKLIVVDGVFSMEGDIVDLPRVVELAKTYGAR
                     IMVDDAHGIGVLGKTGRGTAEHFGLEQEVDLIMGTYSKSLASIGGFISGSEEVIHYIK
                     HSARSLIFSASPPPASVAAVSAAVDIIENEPERLERLWKNTKKMLKGFRDLGFEVGAS
                     ETPIIPIMVGDNKTALKMGMMLQEEGVFANVAVSPAVPDGKALIRTSYMATHTEEQLD
                     NVLHAFRKVGKQMGLIR"
     gene            complement(3548..4513)
                     /locus_tag="VMU21_00110"
     CDS             complement(3548..4513)
                     /locus_tag="VMU21_00110"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUO75953.1"
                     /translation="MKALVTGATGFIGSHLVDELIKKGYGVTCLARQTSSLKWIEGLD
                     VTVLYGDCEDEGSLKDIPSDTQYIFHLAGLTKAKKDEDFFCVNVKGTENLLKAVSGRV
                     PGLKRFIYLSSLSATGPSKNGTPVNETSEPRPVSSYGRSKLEGEGMVAKYRGSIPATI
                     IRPPAVYGPRDKDFYVLFKMLKKGFYPYWGKCYYSLLYVDDLVRGLIMAAEGKETEGG
                     IYFLSDGKVYSNEDIVNEISNVLEAKIVKLRIPKPLMSVLVSISEKLGKESSIINRDK
                     LKELNCFHWVCNSSKAEKDFRFTPKVTIKEGIKWTADWYRIHQWL"
     gene            complement(4558..5397)
                     /gene="htpX"
                     /locus_tag="VMU21_00115"
     CDS             complement(4558..5397)
                     /gene="htpX"
                     /locus_tag="VMU21_00115"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HUO75954.1"
                     /translation="MNQIKTMMLLVVLTLILIWAGAAFGGKQGMTMALIFALGMNFFA
                     YWFSDKIVLRMYRAQEVAEAAAPELYGIVRRLSQKAQIPMPKIYMIQEDQPNAFATGR
                     NPKHAAVAVTTGIMRILSADELSGVIGHELSHVTHRDILVSTIAATIAGAISYLAQMA
                     QWAAIFGHRGDDDEGGNPIAALVMMIVGPIAALIVQMAISRSREYLADEGGARLAGNA
                     RYLSNALRKLNAASQRIPMDANPATSHMFIVNPLSGGGLLKLFSTHPPIEERIARLEA
                     MSF"
     gene            5507..5755
                     /locus_tag="VMU21_00120"
     CDS             5507..5755
                     /locus_tag="VMU21_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75955.1"
                     /translation="MDSGLKIIPLAALFSLTIAINFPFGYFRRRTKRYSFKWFLYIHI
                     PIPLIFLMRTLSHLDFRYIPLFVLAAVIGQLWGGRAEF"
     gene            5837..6772
                     /locus_tag="VMU21_00125"
     CDS             5837..6772
                     /locus_tag="VMU21_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUO75956.1"
                     /translation="MLVKIQSALTCGTDLKAFLRGHPMIPMPGVFGHEFSGVIAEAGR
                     EVKGFRPGDEVIAAHSAPCGRCGYCKKNLFNLCEHIMNTKALGAFAEYILLPEHIVKQ
                     NLFHKPPHLGFEEAAFLEPLACVVHGMSGLRIKKNACVLIIGAGPIGLLHLLMARLRN
                     AKVIISGLEQERLSLAKELGAAMAVVPEELPVAVKEFSPLGVDFVFECTGNVGVWEHS
                     VDYVRRGGSVILFGGCKEGTKVTYDTHRLHYDELTLKGVFHFSPLDVKAAYYLLKNNA
                     LDIPPLISGRYPLTELHTAMEKLSKGEGIKYAIIP"
     gene            6869..>7384
                     /locus_tag="VMU21_00130"
     CDS             6869..>7384
                     /locus_tag="VMU21_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUO75957.1"
                     /translation="MKVAKLYSFSDIRIENIPIPGIGPRDALIKTRACGICSGDIMPW
                     YIEKKAPLVLGHEPAGEIVELGKALLSASGLPLGSVPFSLGDRVFVHHHAPCMECRYC
                     RRGTHVQCETWRSTSIIPGGISEYILIPEINLRNDTLTLPEEISYEDGTLVEPAACVV
                     KSFTRSGIRKGD"
BASE COUNT         1864 a         1919 c         1665 g         1936 t
ORIGIN      
        1 aaagaaaaaa aaaccctcga cccctttgtg cactatgccg ttgctgctgc tgctatggct
       61 gctgaagacg cagggatacc cacctatgcc ccgcttaccg aggggggagt aaggggagaa
      121 aatcactcgt tcctcaattc agcaggggta attatcggct ccagtagggg cggtataagc
      181 acaatagaaa aggcattaat cagccagcac tccctatcac gctcaaatac aagaagatac
      241 cgtctttctc cctatcttat gccttccact accataggca tggctgcatc ttatgtggct
      301 cagaagcttg ggatcatagg gtattgtctc gggatatcca atgcctgctc atccgggaca
      361 aacgccattg gagaggcatt taggctcatt aaatccggtt atgctcattt agtgctcgca
      421 ggcggctcgg aagcgcccct atgcaggctc tgtgttgaag ggtatgggat tgccggtgct
      481 ttgtcgaaac agaaaaggca ctctggagtt ggaccatcaa ctcctaggcc ttttgacaaa
      541 gccagggacg gatttgtatt ggccgagggc tcttgtatac ttgtacttga ggattatgag
      601 agcgcgctag aacggggtgc acccctttat ggcgagatta tcggatatgg aaacaccact
      661 gatgcatttc acattacacg acccagtccc aagggcgaag caaaggcaat gattaaggcg
      721 atagaagaag ccggcttgcg tatggatgac gtggattata ttaacaccca cggaacgggg
      781 acgcaaatcg gagacatggc agaggctcag gcaataaaga tggtctttgg ggagaaaaca
      841 actctaattc ctgcaacggc aataaagtcc ttgaccggcc atatgctcgc tgcgtccggg
      901 gcttttgaga tcgcatcaac gctcatgtct atgagagaag gcattatccc cccaaccata
      961 aacctggatg caaaggaccc tgagtgcgat atcaatgtta tcacagagat gacacctgct
     1021 gacataagag tcgcaatttc aaactccttc ggtttcgggg gggtaaccgc ggtgatcgta
     1081 ttaagaaaga tataaacggg atacaatgcc cctctcggca atccacggag cagctgaaaa
     1141 actcacgtct cgcaaggggc cttagctaaa ggcctctccg cccgttagtt cgggtatcga
     1201 aaccttgttc aggcgctgtc tggcaagtca aataatgagg atgaggcgct atatcggctt
     1261 ctcgtaaacc ctgtacctct tatacagccg tcctcctatc atctccacaa tcctctggac
     1321 cgggatattg tcctcaagta tccatgaaaa ctctactctc ctatatccac cgctctttac
     1381 ccccttaaac ccttccctga aaagaagggc atccaccccc ctatttctgt attccttctt
     1441 tattcccagc agaaggagcc tcaggtcagt gatcttcttt gaatagtaaa gcgcctttaa
     1501 aatgctgacg gggttgagcc tgcccttcat atgcctgagc acataattaa aatccgggag
     1561 aaggcccatg aaccccacgg gctctccatc atcctcggca ataagggtga gctcgggcac
     1621 cgcaatttgc ttcagtctct ccccaaggta ctgcagttct tcgtcagtga gagggatgaa
     1681 accccagttc ttttcccagg ctgagttata gaccttctga aacacccgca tctcttcgtt
     1741 aaacttcttc ttgtccaccg gcctcacctg aatccccctc ttctcagcaa tggccgcaac
     1801 tctcaacacc ttttcgggga ggctctcttt taccgcatag agaaaggcat agaggtcctt
     1861 tgctttttgc atgccgtact gctccatcat gttattgtaa tagggcggat tatggggtgt
     1921 cataagcatg ggaacattgt ggaatccctc gacgaggaat ccgcactctt cgtttgtgga
     1981 aaaattcatg gggcccctca taatggccat gccctttccc ctgagcgcgt cactcaccgc
     2041 atcgaggagg caatgggata cctcccgggc attcacagat tcaaaaaatc caaaaaaccc
     2101 taccctttca ttatggaagt ctatgtgcct ccggttgatg atagagacca ctctccccaa
     2161 ggtcttccct ttctttctgg caagaaaata tcttacctct gcatgctgga agaacgggtt
     2221 tttaattgag aattgctcct tcatctcgcg ttttagtggc ggagaataaa gcgggtcttg
     2281 agcgtaaagg gaatagggga aattgatgaa atcagcgagg tctctttctg atcgaacctc
     2341 gagggccttc aatgggctca gcgtataagc cccatttgct ttccgacttt tctaaacgca
     2401 tggagcacat tatccagttg ctcttcggta tgggtagcca tatagcttgt ccttatgagc
     2461 gcctttccat caggcactgc ggggctcacc gccacgttgg caaatacccc ttcttcctgg
     2521 agcatcatcc ccatcttcag tgctgtctta ttgtcaccca ccataatggg tattatgggt
     2581 gtctcgctcg caccgacttc aaatcccaaa tctctgaacc ctttgagcat tttctttgta
     2641 ttcttccaga gcctttctaa tctttccggc tcgttttcga taatatcaac ggctgcactc
     2701 actgcagcca cagaagcagg aggggggctt gcgctgaaga tgagcgaacg ggcagagtgc
     2761 tttatgtaat ggatgacctc ctcggatcct gatatgaatc cgcctatgga ggcgagggac
     2821 ttgctgtagg tgcccatgat caggtccacc tcttgctcaa ggccaaaatg ctctgcggtc
     2881 cccctccccg tctttcccaa gactcctatc ccgtgggcat cgtcaaccat aatccttgca
     2941 ccataagttt tcgcaagttc taccaccctg ggcaaatcca cgatatcccc ttccatgctg
     3001 aacacgccgt cgacgacgat aagttttccc ctcccattgt tctgatcgag aatcctctct
     3061 aagttgtcca tatcattatg cttgaatttc ttgacctccc cgtaagagag cctgcatcca
     3121 tcgataatgc tcgcgtggtc catcttgtcg ataattacta tgtcgtcttt tcccacaagg
     3181 gccgagatca cccccaagtt cacctgaaac cctgtggaaa agacaacggc tacctctttt
     3241 cttataaacc tcgccagttt tttctccaac tccacatgga tattgagcgt gccattaagg
     3301 aacctcgatc cggcacatcc cgatccgtat ttccgtatgg catcaatagc cgcttctttg
     3361 accttcgggt ggtttgtaag accgaggtaa ttgttggaac ccaccataat caagcgtttg
     3421 ccgtgcatca tcacctcagg gtcctgtgcg ctctcgatca ccctgaaata agaataaagc
     3481 cctgctgaca ttagctcctt tgccttggtg aatttgccgc acttctcaaa aatgtctatg
     3541 gtcttcttta cagccattga tggatcctgt accaatctgc tgtccatttt atcccttcct
     3601 ttatcgtaac ttttggggta aagcgaaaat ccttctccgc ctttgatgag ttacaaaccc
     3661 aatggaagca gttaagctcc ttaagcttat ctctgtttat tatggatgat tctttcccca
     3721 gcttctcgct gatactcaca agcactgaca taagaggttt cggtatccgc agcttcacta
     3781 tctttgcctc cagaacattt gatatctcat ttacaatatc ctcattggaa tagacctttc
     3841 catcggacag aaagtatata ccgccttcgg tttcctttcc ctcggcggcc ataatcaatc
     3901 cccttacgag atcatctaca tacaggagtg aataataaca ttttccccaa tagggataaa
     3961 agcctttttt tagcatctta aataagacgt aaaaatcctt atcccttggt ccgtataccg
     4021 caggcggccg tatgattgtt gcgggtatac tacccctgta cttggccacc atcccctccc
     4081 cctccagttt acttcttcca taactggaaa caggcctggg ctccgaagtc tcattcaccg
     4141 gcgtaccatt cttgctaggg cctgtggcag aaagactact gagatagata aagcgcttga
     4201 gccccggaac tctcccggat accgctttca ggagattctc tgtgcctttc acattgacac
     4261 agaagaagtc ttcatccttc ttggcttttg tgaggcctgc cagatgaaag atatactgag
     4321 tgtcagaagg aatatcctta agagacccct cgtcctcaca atccccataa agaaccgtca
     4381 catcaaggcc ttctatccac ttaaggctag aggtctgtct cgcaagacag gtaaccccat
     4441 atcccttctt gataagttcg tcaacaaggt ggcttccaat aaagcctgtt gcacctgtca
     4501 caagggcctt cataggacgt cgttattttg aacaatcatt accttaagag tcaagcgcta
     4561 aaaactcata gcctcaagcc tggcgattct ctcctctatg ggtggatgag tactaaaaag
     4621 cttaagtaat ccaccccctg aaaggggatt cacaatgaac atatgggagg tggctggatt
     4681 tgcgtccatg ggtatccttt gggatgcagc gtttagcttg cggagcgcat tcgaaaggta
     4741 tcgggcatta cctgccagcc ttgccccgcc ttcatcagcg aggtattccc tcgatcttga
     4801 tatggccatc tggacaataa gggccgcaat gggccccaca atcatcatta cgagggctgc
     4861 aattgggttc cctccctcat catcatcccc cctgtggcca aaaatggcag cccattgggc
     4921 catctgggca agataactga tggctcccgc gattgtcgca gctatggtac ttacgaggat
     4981 atctctgtgg gtgacatggg aaagctcatg gcctatcacc cctgaaagtt cgtcagcgct
     5041 gaggatcctc attattccag tggttaccgc cactgcagcg tgctttgggt ttcttccggt
     5101 ggcaaacgca ttgggctgat cttcctgaat catgtaaatc ttgggcatag gtatctgggc
     5161 tttttggcta agcctccgga caatgccgta aagctcaggg gctgcggcct ccgctacctc
     5221 ctgtgcacgg tacatcctga gcacaatctt atcgctaaac cagtaggcaa aaaaattcat
     5281 gcccaaagca aaaataagcg ccatggtcat gccctgcttc ccgccaaagg cagcacctgc
     5341 ccatataagg atgagggtta acactactaa cagcatcatt gtttttatct gattcatgga
     5401 aatacctcct ctgtcgcatt aactctataa taataacgaa aaaacaggac aaattcaacc
     5461 ttagtggagg tttgacttat cctgctccca taggttaatt ttctatatgg attcaggttt
     5521 aaagattatc cctttggcag cacttttttc tctgaccata gccattaact tcccatttgg
     5581 ctattttcgg aggaggacaa aacgctattc attcaaatgg tttctctata tccacatccc
     5641 tatccctctt atcttcctca tgaggacgct ttcacacctt gatttcagat acattcccct
     5701 gttcgtcctt gccgcagtta tcggtcaact ctggggcgga agggcagagt tctagccggt
     5761 gctcgcagga atccttgtct caccaggaag aatagaaata cgggaaacgg agaccccgaa
     5821 accttcaagg agtgaagtgc tcgttaaaat tcaatcagcc ctcacctgcg gtactgatct
     5881 caaggcgttc ctgagaggac atcccatgat acccatgcca ggggtattcg ggcatgagtt
     5941 ttcaggggtc attgctgagg caggccgcga ggtcaaggga tttaggccag gggatgaggt
     6001 aatcgcagca cacagcgctc cgtgcggaag gtgcggctac tgcaaaaaga acctatttaa
     6061 tctctgcgag cacattatga acacaaaagc attgggcgcc tttgctgaat atatcctctt
     6121 gcccgaacat atcgtgaaac agaatctctt tcataagcct ccgcacctcg gatttgaaga
     6181 agcggcattc cttgaaccac tggcatgcgt agtccatgga atgtccggcc tccgcattaa
     6241 aaaaaacgcc tgcgttctca taataggtgc aggccctata ggacttctgc accttctcat
     6301 ggcaaggtta agaaatgcaa aggtgatcat ttcaggattg gaacaagaaa gactaagcct
     6361 ggccaaggaa ttaggtgctg ctatggccgt agtccctgaa gaactccccg ttgcggtcaa
     6421 ggaattcagt ccccttgggg ttgattttgt ctttgaatgc accgggaatg tgggtgtatg
     6481 ggagcattcc gtagattacg taaggagagg agggagcgta attctatttg gaggctgcaa
     6541 agaaggaaca aaggttacct acgatactca caggctccat tatgatgaac tcacattaaa
     6601 gggagtcttc catttttcac cccttgatgt gaaagcagcc tattaccttt tgaaaaataa
     6661 tgccctagat attcctcccc tcataagtgg ccgttatccc ctgacagaac tccatactgc
     6721 tatggaaaaa ctttcgaagg gagaagggat aaaatacgcc atcattcctt aatgcataag
     6781 agtttgggaa agtacaagat gaaaaaaata gcgtcaatca ttgttctgtc ttgtcatcaa
     6841 ccctatgcac aaggccaggc ttgagcaaat gaaagtcgcc aaactctata gttttagtga
     6901 catacggatt gaaaacattc ctatcccagg aattggcccc cgtgatgcac tgataaaaac
     6961 gagggcctgt ggcatttgtt cgggagatat catgccttgg tatatagaaa aaaaagcgcc
     7021 ccttgtcctg ggtcatgaac ccgcgggaga gattgtggag ttgggaaaag cgctgttaag
     7081 tgcttcaggc cttcccctcg gctctgtgcc attttcacta ggcgacaggg tctttgtgca
     7141 tcatcatgcg ccatgcatgg aatgcaggta ctgcagaagg gggacccatg tgcagtgcga
     7201 gacgtggcga tcaacatcaa ttattcccgg gggcatttcc gaatatatcc tcattcccga
     7261 gataaacctg aggaatgaca cccttaccct tcctgaggag ataagttacg aggacggcac
     7321 tctggtggaa cccgctgcat gtgtggtcaa atcattcact aggtcaggaa taaggaaagg
     7381 agac
//
LOCUS       DATKOU010000006         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3858427, whole genome shotgun sequence.
ACCESSION   DATKOU010000006 DATKOU010000000
VERSION     DATKOU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858427"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..751)
                     /locus_tag="VMU21_00135"
     CDS             complement(152..751)
                     /locus_tag="VMU21_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUO75958.1"
                     /translation="MNKRSGKESKKKILSAATRVFSEYGYKGASMRMIARSADISIGG
                     LYLYFKNKEDLYTTLIRISLNDLAGETREALGDIDDPAEAIRTFITMRVHYAKKHREL
                     ILILGKEQGFTFGIKARKQFFREQRKVIEEIVRKGITSGRIRKCKVEEVAKIIICALR
                     GFILSMLVEQDALFSPEECGTLVLEGLLAKEPESPFPKR"
     gene            1148..1696
                     /locus_tag="VMU21_00140"
     CDS             1148..1696
                     /locus_tag="VMU21_00140"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HUO75959.1"
                     /translation="MMTKKVLFAVALLTVFLFALSAGVLVAQDKYALKAPNGISFSEI
                     RGYETWQVIAPSYRTEKKELRIILGNPVMINAYKEGIPGNGKPFPEGSIIVKVGWSER
                     KNAAFPAAIEPDVLQRVEFIVKDSKRFPDTNGWGYARFVYDAKTGIFSPYGKDASFMQ
                     ECHQCHTLVKAKDFIFTGYPQR"
     gene            1747..2709
                     /locus_tag="VMU21_00145"
     CDS             1747..2709
                     /locus_tag="VMU21_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HUO75960.1"
                     /translation="MKAIRVHKFGEPEVMKLEEVDEPKPGTGQVLVKVHAVGVNPVDC
                     YVRSGLYPLKPALPFTPGMDAAGIVESVGEEVKRVKPGDRVYVAGTVSGSYAEKALCE
                     VSRVFPLPQNVSFAQGAALGVPYGAAYRALFHRAKAIPGEVVLVHGASGGVGIAAVQL
                     ARAAGMQVIGTAGTESGRQLVREQGAHHVFDHLSPDHMKQILDLTEKRGMDVILEMLA
                     NVNLGKDMGILAQDGRVVVIGSRGTVEIDPRDAMRRDAAILGMVLMNASEQDIYRIHA
                     ALGASLEAGVLQPVIGKEFPLAEGPRAHHEIMESVAHGKIILVP"
     gene            2726..2932
                     /locus_tag="VMU21_00150"
     CDS             2726..2932
                     /locus_tag="VMU21_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2762..2764,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HUO75961.1"
                     /translation="MARHIIIFGKAGUPYTDRARSAYGDTAQYFDVQTDATKLDEMLK
                     YSGGVRKVPVIVKGDKVEIGYGGS"
BASE COUNT          826 a          820 c          904 g          810 t
ORIGIN      
        1 cagcatcacg aatgtgatga tgaagaacaa gggtatcttc tcttcctctg ctctcaaatc
       61 tctctgggtt gacatgtgcc tccctgcttt ctccgaatct tgacgccccg cactgcacgc
      121 ctgtaatctt aacccacctg ccgccaaact ttcatctctt agggaaaggg gattccggtt
      181 ccttcgccag cagcccctca aggacaaggg taccgcattc ctcaggagaa aacagggcat
      241 cctgctccac gagcatggag aggatgaatc ccctcagggc acagataatg attttcgcga
      301 cctcctccac tttgcacttc cttatcctgc ccgacgtgat cccttttctc acgatttctt
      361 cgattacctt cctctgttcc ctgaaaaact gttttcgagc ctttatccca aaggtgaatc
      421 cctgttcctt ccccaggatg aggatgagtt ccctgtgttt ttttgcataa tgcacccgca
      481 tggtaataaa ggtcctgatg gcctctgcag gatcatcaat gtcacccagg gcctctcttg
      541 tctctcctgc caggtcgttg aggcttatcc tgatgagagt ggtatagaga tcttccttgt
      601 ttttgaaata gagatagaga ccgccgatgc taatgtctgc tgaccgtgcg atcatcctca
      661 tgcttgcccc tttgtaccca tactctgaaa ataccctggt ggcggcactg aggatcttct
      721 ttttcgattc ttttcctgaa cgcttgttca tgtgaaccag tgtttagtat agacaagacc
      781 gtttttcggt gtcaacagag gtcacatggc tgtgtacggc agcactcccg gaaaagagac
      841 ctcgatcttg aggaaaatct tagcaaggaa cgtgctcaag ggtaccgtcc ctgcgccaat
      901 cctgaaacgg atagggagtg cattcccctc agtccgcctc aggcggactg aggagttaac
      961 gagcgaatat gagagagagt cattccctac gttaagacac cctgtcccga aagccttcgg
     1021 aagcgtaggg ttcttcactc tcaaggactt gcaggtgcat tgtttttgtg atatgaagta
     1081 aagagaatcg gtgaaactca ttttgggaat acaggcagga aataaagaaa agaaggagga
     1141 ggcctctatg atgacgaaaa aggttttgtt tgcagtcgca ctccttacag tattcttgtt
     1201 tgccttgagt gccggtgtac tcgtagccca ggacaagtac gcactcaagg cccccaacgg
     1261 aatatctttt tccgagatca ggggatacga aacctggcag gtgatcgccc cgagctacag
     1321 gaccgagaag aaagaattaa ggatcatcct cgggaacccc gtaatgataa atgcatacaa
     1381 ggaaggtatt cccggcaatg gcaagccctt ccccgagggg tcgatcatcg tgaaagtcgg
     1441 ctggtcagag agaaagaatg cggcctttcc cgctgccatt gaaccggatg tgctgcagag
     1501 ggttgagttc attgtcaagg actcaaagag atttcccgac accaatggct ggggatatgc
     1561 tcgttttgtc tatgacgcaa agacaggcat attcagcccg tatggaaaag acgcttcctt
     1621 tatgcaggaa tgccatcagt gccacacgct ggtgaaggca aaggacttta tatttacggg
     1681 gtatccccag agataaaggg acgaaaccct catttcacag aaattatgca ggtggaaagg
     1741 agaccaatga aagccatccg ggttcataaa ttcggcgaac ctgaagtaat gaaacttgag
     1801 gaagtggatg aacccaaacc cggcacgggc caggtcctcg tgaaagtcca tgctgtgggg
     1861 gtaaacccgg tggactgcta tgtccgctca ggactgtatc ccctgaagcc tgcgttgccg
     1921 tttaccccag ggatggatgc agcgggaatt gttgagtctg tgggggaaga agttaagcgt
     1981 gtgaaacctg gcgacagggt gtatgtggca ggtacggtga gcggttcgta tgcggaaaag
     2041 gcgctctgtg aagtatcccg ggtcttcccc ttgccgcaga atgtatcttt tgctcagggg
     2101 gctgcattgg gtgtgccgta tggcgcggca tatcgcgccc tgttccatcg cgcaaaggcc
     2161 attccggggg aggtcgtcct cgtacatggc gccagcggag gtgtgggaat agcagcagta
     2221 cagcttgccc gcgcagcagg gatgcaggtg atcggaaccg cgggtactga gagcggcaga
     2281 cagcttgtga gggaacaggg tgcccaccat gtgtttgatc acctttctcc tgatcatatg
     2341 aaacaaattt tggacctgac cgaaaaacgt ggtatggatg tgatcttaga aatgctcgca
     2401 aatgtgaatc tgggaaagga tatggggata cttgctcagg acggacgggt tgtggtgatc
     2461 ggcagtcgcg gcactgtgga gatagatccc cgcgacgcaa tgaggcgtga cgctgcgata
     2521 ctcgggatgg tgctcatgaa tgcctctgag caggacatct accgcatcca tgcggccctt
     2581 ggagccagcc ttgaagccgg cgtactacaa cccgtcattg gaaaggagtt cccccttgcg
     2641 gaaggtccgc gtgcccacca tgagatcatg gaatccgtgg cacacggaaa aataattctt
     2701 gtcccataaa ctataaggag gtgtcatggc cagacatata atcatttttg gaaaggctgg
     2761 ttgaccttac actgacagag ctaggtcagc ttacggggat accgcgcagt attttgacgt
     2821 gcaaaccgat gccacaaaat tggatgaaat gctcaaatat tcgggcgggg tgaggaaggt
     2881 acccgttatc gtgaagggcg ataaagtgga gatcgggtat gggggaagct gaggggtata
     2941 gccaccggca ggtgccggtg atgcaacctc gactcaaagg aggcagggga tatcaacgta
     3001 atcttaataa ttcataagtt caagaggctc gcccgctttg cggcaatata caatagcccc
     3061 tgtgccttgt tgctatcatt ttcgcctaat tactgtaatg aattaccggc ctcatgcggc
     3121 ctcacacccg gccgccccaa taactccagg tattttgcat taaacctggc tagcctctta
     3181 ggttccactt ttatgaatgc tgagagaggc attcggttcg aacgcaagag tccgagttag
     3241 gtgccagtcc gctcatacgg actgataaga ccctatttct ggacagaccg atcttaggtt
     3301 cactctgaaa cagtttggaa caaggggcta ttctcttgcg gagaaacgga gtctctctca
//
LOCUS       DATKOU010000007        12828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3859038, whole genome shotgun sequence.
ACCESSION   DATKOU010000007 DATKOU010000000
VERSION     DATKOU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859038"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VMU21_00155"
     CDS             <1..265
                     /locus_tag="VMU21_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75962.1"
                     /translation="VKEMTAQEGHREHVYGKLVMAPVLGLLYVIALPFIAIGTVAAVM
                     GKKAVEGLYHVVGNLVSFGWRPLEANLSGKKKERKRKEKDSSK"
     gene            336..734
                     /locus_tag="VMU21_00160"
     CDS             336..734
                     /locus_tag="VMU21_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75963.1"
                     /translation="MHTLFDFMTHIKGVEYILALMFIAGYILFSEVLKPKPFATVKST
                     GKDDLEYVRKVGYRGIMKTIGKIAAAPFIGIAYVVMLPFAFVAALGTEVGGRVMSLAG
                     RSTAFGWRPMESYLTGKKTKKEDKKQEEKK"
     gene            complement(859..1149)
                     /locus_tag="VMU21_00165"
     CDS             complement(859..1149)
                     /locus_tag="VMU21_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75964.1"
                     /translation="MGLIQRAIEHIGIPTIGISIVREYTVKVKPPRTIYLRWPFGHPL
                     GEPFKISQQKAVLAEAFKALYAIDKPGEIIDIPFKWRRQRYDNYSIEELKNF"
     gene            complement(1168..1686)
                     /locus_tag="VMU21_00170"
     CDS             complement(1168..1686)
                     /locus_tag="VMU21_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HUO75965.1"
                     /translation="MTTFSRFKNRFIAKLLVRLPFLAKRSVAAYHPPESREIPWTPVA
                     KKLNESIVALVTTAGVHHRDQKAFDMKDPNGDPSFRVIDVTKPFADLMITHDYYDHAD
                     ADRDMNIVFPVDRLREFEQEGLIGKLADAHYGFMGHIMGPHLHTLINKTAPEVAHLLK
                     TAGVDIVLLTPG"
     gene            complement(1702..1848)
                     /gene="ccoS"
                     /locus_tag="VMU21_00175"
     CDS             complement(1702..1848)
                     /gene="ccoS"
                     /locus_tag="VMU21_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HUO75966.1"
                     /translation="MWTIGFLIFLSIILGIGAWLFFIWSVKSEQYDDIEGPKHRMLDD
                     DDEK"
     gene            complement(1853..4297)
                     /locus_tag="VMU21_00180"
     CDS             complement(1853..4297)
                     /locus_tag="VMU21_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HUO75967.1"
                     /translation="MLQCNHCLLDFPDREAVHDTIDGQEKVFCCWGCSGIFRLIHQEG
                     LDAFYEKRKWNERGISSSLFQREPDIKPFAEYVIESGNDREIDLYIEGIRCASCVWLN
                     EKILVKTEGVEYARVNYATHRAKIRWDPNRIGLERILKRILSIGYIPKPFSESEQFRV
                     QQAETKDLLVRLGTACFLSSQLMIYSIALYAGYFQGIDPGTKHVLELIAMLLTIPVLF
                     YSGMPFVRSTINGFKHLRFTMDSLIIIGAGSAFIYSIYEIFSGGEVYFDTSAMIITLI
                     LLGRYIEAAAKGKASETVKLLIELSPKTAVKLVRNQETGVNERETVLLTSLSKGDLVE
                     VKPGEKIPLDGTVIAGESESDESLLTGESRPIPKTPGTEVMGGSMNLYGTLTIEVTRT
                     AKDTVLAGIIRAVEDAQTHKPRIQALADRVVGIFVPAILLIALATVISYLARGSSLHH
                     SLMTGVSVLVIACPCSLGLATPLAVLIFTAMASSRGILIRSSEVIENAARLGHIVLDK
                     TGTITKGRPLLKEVIIADHSLARDHLLSVAASVECMSEHSIGHAICEAAIPAFQITGF
                     KAFQGKGVEGHAEGRRIVIGNRVLMQEQGIDMTALGPLNGSAADFEGQGDTVIFMGWD
                     GAVRALLVISDIIRDEVPEAVDSVKAMNCNVAIVSGDTDITTRSIASQVGITERLSGT
                     SPVGKRDYIAGIQLEKRRVMMVGDGINDAPAITEASVGIAMGRGTEIAIESADAVLIR
                     NDLRLIPYFITLSKKTYGVIKQNIFWAFFYNIAAIPVAVSGVLHPIIAAGAMAASSLF
                     VVCNSLRIRNQKVNRE"
     gene            complement(4284..4958)
                     /locus_tag="VMU21_00185"
     CDS             complement(4284..4958)
                     /locus_tag="VMU21_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO75968.1"
                     /translation="MNTGYILAFFTGLLGGLGHCIGMCGPLVASYTLYGKDSLRRFSA
                     LLPHLLYNAGRITTYVFIGALMGISGSFVNTATKLSGIQSAVGILAGAFMVLMGLGIT
                     GVFKNIRLIEKHNSLILHMVKNVREGDSLWRYFPLGLLLGFLPCGLSYSVFIGAAATG
                     SMPSAMFFVLCFGVGTIPSLLLFGLAMTYLSVRIRGWIYLTSGVMVIITGVYFLARSF
                     IYHASV"
     gene            complement(4958..5416)
                     /locus_tag="VMU21_00190"
     CDS             complement(4958..5416)
                     /locus_tag="VMU21_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HUO75969.1"
                     /translation="MKRSLAVTIVGVWAMLLTLGCSKPHDAAGTDCRIDKDPCVKTIM
                     PGNITAAFDIQPKPVKMMSGLYFNLSLSKWGRPISDADIILHLTMPGMYMGERKVIMS
                     HKGGGRYEGSSIIPRCPSGRRIWKAAFVINETNTVPSGTVSAEYTFEVKE"
     gene            complement(5413..5859)
                     /locus_tag="VMU21_00195"
     CDS             complement(5413..5859)
                     /locus_tag="VMU21_00195"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HUO75970.1"
                     /translation="MKILIGIVIMVALAAVVTAVIVGNKSFEGIVVDKPYERGLSWDR
                     EQKERTESGLNIEIKNKTFTVGENDLLIQVTDREQKPVPDAILMLTVSRPSSSAYDRT
                     YRFSGGEGGVYSAPVALPLYGHWDLRITIVRNGQNLLFPQKIFVEQ"
     gene            complement(5856..7070)
                     /locus_tag="VMU21_00200"
     CDS             complement(5856..7070)
                     /locus_tag="VMU21_00200"
                     /inference="COORDINATES: protein motif:HMM:NF023045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO75971.1"
                     /translation="MTTIKRIQPLRRTAEILQAVLIIGIPFLTINGESALRFDIPSLR
                     LHVFGVSLWMDEFFLVLIALIFVTFFTVLVTLMFGRIWCGWTCPQTVLVDFTRLLDKT
                     YSKGITYRLMAYAVTFLISLIVAANLIWYFVSPYEFFHRLYSGTLGNVIGGFWCVMTI
                     ILFLNLLLLRQTFCATVCPYAKLQSVLFDRRTLVISFDQRRQEECMNCMACVTTCPVK
                     IDIREGTNAACVSCAECIDACARMMSRRQKKGLIDYFFSSPGQRPRLLRQSVVLTTSL
                     AIVFLFLFLYLSISRAPLDMMVLPHYAFPPRVLTDAKVVNSYILSLENKSRTGKEVTL
                     QVRRPKGSISITPERVHLDAGEYKKVPVYVTVGQIFPGATSEDIEILLESEDPDRVII
                     KKGVRFTIPKTQ"
     gene            complement(7057..7200)
                     /locus_tag="VMU21_00205"
     CDS             complement(7057..7200)
                     /locus_tag="VMU21_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HUO75972.1"
                     /translation="MTPQSIAYILFGLVLVILFGIIIVFYYSKKRHKKVEEAKYKMMD
                     DDD"
     gene            complement(7394..7588)
                     /locus_tag="VMU21_00210"
     CDS             complement(7394..7588)
                     /locus_tag="VMU21_00210"
                     /inference="COORDINATES: protein motif:HMM:NF026065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HUO75973.1"
                     /translation="MDDKLDELSEFLVEESVDAKRKIPIGWLILFFGLILWGIYYFVS
                     YTPSISGWSQEKAYEESVKK"
     gene            complement(7604..8548)
                     /locus_tag="VMU21_00215"
     CDS             complement(7604..8548)
                     /locus_tag="VMU21_00215"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HUO75974.1"
                     /translation="MANELHHKLHTSPILFAVLATVAILVGTVVTMFFPMLTQQMHPK
                     LENLKPYAALQLAGKDIYQREGCNNCHTQTVRPLKTEVMRYGEYSKAGEFAYDRPFLW
                     GSKRTGPDLARIGEKYPDAWHYGHFENPQAFFAESNMPAYGWLRNNKLDPSDIEAHMK
                     ALGLPYAPADITALGKRTEMDALVAYMQVIGTSVKKKPEAVAGPAVKETHIPNPLAGD
                     PKAIALGKEIFKANCAVCHGENAKGSIGPSLVDNEFLYVAGDVPDDDYFEVIHNGTQP
                     GMIEDGRAAKGGMPPFKGTLDKDRIWSVVAYIRSLQGK"
     gene            complement(8560..9957)
                     /locus_tag="VMU21_00220"
     CDS             complement(8560..9957)
                     /locus_tag="VMU21_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HUO75975.1"
                     /translation="MSDYHYDNQTVKGFIVSSIFWGVVGILIGLWISVQMWKPEWNIP
                     PFFTFGRMRVVHTNGLAFGLGIGAIFGISYYVVMRLTRRPLLFPKLARFHLYLFNVAI
                     TLAALSLFAGMNQSLEYAELEWPLDIVVVILWVIFAVNIFGTIIKRKEEQMYVSLWYI
                     IATVIAVAVLYIVNNLSIPAGLFKSYHLFAGVNSANVEWWYGHNAVGFIFTTPILAMF
                     YYFLPKATGIPIYSHRLSIIAFWSLVFAYLWTGAHHLVYTPLPDWIQTLAIVFTIFLI
                     APSWGSVVNGYYTVGSDWSKMQTNYLTKFFILGITFYGLQTVQGPTQGLRVVSQLIHY
                     TDWVPGHVHMGTMGWVTMTVAASIYYIIPKIYDTEIYSIKIANTHFWLVLIGQLMFSI
                     TMWITGIQQGTLWKAVTPDGSLKYTFIETLVRNYPFWKLRTVAGLIFTIGMLFFIYNI
                     YMTMRKGKALAAAKA"
     gene            complement(9969..10436)
                     /locus_tag="VMU21_00225"
     CDS             complement(9969..10436)
                     /locus_tag="VMU21_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HUO75976.1"
                     /translation="MEITRETDYAIRCVLYLSGTKEAVIMADEISRAMSIPKSFLAKI
                     LQKLTKAGVVTSFRGVKGGFRLAKKPQQISLLDIIEAIEGPAAMNRCAVDASICNLSG
                     TCTVHPVWVKLRKLVENSLRKTNFRGLSSSTSLKRKQRAYNARKDSVRVRNNH"
     gene            10634..11482
                     /locus_tag="VMU21_00230"
     CDS             10634..11482
                     /locus_tag="VMU21_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HUO75977.1"
                     /translation="MHGLNFETHGSPVYRLYDREGTEIAYDEAIDRLAGAEVVLFGEL
                     HDNTLIHQTQISMTEDLYKKRKGELILGAEMFEADNQLILDEYLAGLILHRHLVAEAK
                     VWDNYETDYRVLVDFAKKHGLKFIATSIPRRYAGLVSREGIKALDGLSGEAKQYIAPL
                     PIAVALATPGYREMMEMNLDMGHGMQMNPENLVAAQAVKDATMAYFILKNRASKQLFL
                     HFNGVYHSQNYGGIYWYLKKVAPELNVLALCAVEEESFEFQDEYKGLGDFILVVPAAG
                     PPPPRT"
     gene            11883..12806
                     /locus_tag="VMU21_00235"
     CDS             11883..12806
                     /locus_tag="VMU21_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75978.1"
                     /translation="MKRVWLLIAMLLLAGCGSGGGSNVSNSQPGGSQPGGIIEVGPST
                     PTGNVVVNIVEGDAKTSAAIPPDDHVRLVVTNPNLRINGSVYKLIVDGLNPGGHITGL
                     KFPVATGYTFELITYAPDHVQPLGTVPINRMVQHAKTPVPVNITPDGATVDLTINTIT
                     ACINDIPTPAYSNAPLGITATFTLQPTPLQPGWNLFLNTSLPLMSHALHSTATPIANH
                     ALVKAPIVLTEQTLYAQAEFYINQSLLDTTGLLDILNPITMASPPTLAAPHAAENIHD
                     WTFNCPNPDAGDDPSAHSVFLQLVGVSIPTP"
BASE COUNT         3268 a         3120 c         3152 g         3288 t
ORIGIN      
        1 ggtgaaggaa atgacagcac aggaaggaca tagggagcat gtatacggga agttggtaat
       61 ggcaccggtt cttgggctgc tgtatgtaat cgcactgccg ttcatcgcca ttgggacagt
      121 ggcggcggtg atgggcaaga aggcagtgga agggctttac catgtggtag gcaatctggt
      181 gtcttttgga tggaggccat tggaggcaaa cctttcgggc aagaagaagg agaggaagag
      241 aaaggaaaag gattcctcca agtgagggga tgaataacta tagtaaatca taaacaaaat
      301 gtcgtcatag acggcacaaa acaaggaggg agaaaatgca tacactgttt gatttcatga
      361 cccacatcaa aggagtagag tacatcctgg cattaatgtt cattgcgggg tatatcctgt
      421 tttccgaggt actgaagccg aagccgtttg caacggtaaa gagcactggc aaagatgacc
      481 ttgagtatgt gaggaaggtc gggtaccggg ggataatgaa gaccataggc aagatagcgg
      541 cagcgccgtt cattggtatt gcctatgtag tgatgcttcc ttttgccttt gtggcagcgc
      601 ttggcaccga ggtgggcggc agggtgatga gccttgcggg caggagcact gcatttggat
      661 ggaggccgat ggagtcgtat ctgactggca agaagacaaa gaaggaagac aagaaacagg
      721 aagagaagaa ataactacct tcaccctggc agttcccgag aacccttcac acaggaagta
      781 ccgggagctg ccaataaacc acattttttg atgacctctc agcttcatta attctaggaa
      841 aaccaggtct catctttttc aaaaattctt gagttcctca atggaataat tatcgtatct
      901 ctgcctcctc catttgaagg ggatgtcaat tatttctccc ggcttatcta tggcataaag
      961 tgccttaaag gcctctgcaa gcacggcctt ttgttgtgag attttgaatg gctctccaag
     1021 gggatggcca aaaggccacc tgagatagat ggtgcgtgga ggttttacct tgacggtata
     1081 ttcccgaaca attgatatac ctatggtggg gatgccaatg tgttctatcg ccctctgtat
     1141 cagtcccacg gactggttgc agattcctca gccaggtgtg agaaggacaa tatctacgcc
     1201 tgctgttttc aggagatggg caacttcggg tgcggtcttg ttaatgaggg tatgcaggtg
     1261 gggccccata atatgaccca tgaagccata atgcgcgtct gccagtttgc ctattaagcc
     1321 ttcctgttca aattccctca gcctgtccac cggaaataca atattcatgt ccctgtctgc
     1381 gtcagcgtgg tcataatagt catgggtaat catgaggtcg gcgaacggtt ttgtcacatc
     1441 aatcaccctg aatgaggggt caccgttggg atctttcatg tcaaaggctt tttgatctct
     1501 atgatgcacc cctgctgttg tgacgagtgc gacaatgctt tcgttcagtt ttttcgcgac
     1561 cggtgtccag ggtatttccc ttgattcggg tggatggtac gcggcaacag aacgtttggc
     1621 aagaaacggc aggcggacaa gaagttttgc aataaaccta ttcttaaaac gtgaaaatgt
     1681 tgtcataaga ctttctgcac attatttctc gtcatcgtca tcgagcattc tgtgctttgg
     1741 cccctcgata tcatcgtact gttcgctttt cactgaccat atgaagaaaa gccatgcgcc
     1801 gataccgagg attatggata aaaagatcag aaaccctatg gtccacatac gatcactctc
     1861 tattcacctt ttggttcctt atcctcaatg aattgcacac tacaaaaagt gaacttgcag
     1921 ccatcgcgcc tgccgcaata ataggatgga gcaccccgga aactgcaaca gggattgcag
     1981 cgatattata aaagaaagcc cagaatatgt tctgcttgat caccccatag gtcttttttg
     2041 agagggttat gaagtagggt ataaggcgca ggtcattcct gatcaacacc gcatccgcgc
     2101 tctcaatagc gatctctgtt cccctgccca tagcgatgcc tacagatgcc tcggtgatgg
     2161 caggggcgtc attaatgcca tcgccgacca tcattaccct gcgcttctcc agctgtattc
     2221 ccgcaatgta atcccttttt ccgaccggtg atgtcccgga tagtctctcc gtgataccca
     2281 cctgggaagc aatggaccgg gttgtgatat ccgtatcacc gcttacaatg gccacattgc
     2341 agttcatcgc ttttacggaa tccactgcct caggaacctc atctctgatt atgtcggaga
     2401 ttaccagtaa tgcccgcacc gctccatccc atcccatgaa aatgaccgtg tcaccctgtc
     2461 cttcaaagtc cgctgcagac ccattgagag gcccgagggc cgtcatatca attccctgct
     2521 cctgcatcag tacacggttg ccgatgacta tccttcttcc ctccgcatga ccttccacac
     2581 cttttccttg aaaggctttg aatcctgtta tctgaaaggc aggaatcgcc gcctcgcata
     2641 ttgcgtggcc tatgctatgt tctgacatgc attcaacaga ggcagcaact gaaaggagat
     2701 gatcccttgc aagggaatgg tcggcaatga tcacctcttt tagaagtggc ctccccttgg
     2761 tgatcgtgcc cgtcttgtca aggacaatat ggcccagtct cgcggcgttc tcgattacct
     2821 cgctgctccg aatgagaatt ccccttgaag aggccattgc ggtgaagatg agcaccgcga
     2881 gcggcgtggc gagcccgagg ctgcagggac aggcgatcac aaggaccgaa acacctgtca
     2941 tcagggagtg gtggagggaa gatccccgtg cgagataaga tatgacggtt gcaagggcga
     3001 taagcaatat ggcagggacg aatatgccca ccaccctatc cgcaagggcc tgaatccttg
     3061 gcttatgggt ctgtgcgtcc tccactgccc tgatgatgcc cgcaaggacg gtatcttttg
     3121 cggtccgtgt gacctctatt gtgagtgtgc cgtaaaggtt catgcttcct cccatgacct
     3181 cagtacccgg ggtctttggt ataggcctgg attcacctgt gaggagggat tcatcacttt
     3241 cagactctcc tgcgatgacc gttccatcaa gggggatctt ttctccgggc ttcacttcca
     3301 ccagatcacc tttcgaaaga gaggtgagca ggaccgtttc cctttcattc actcctgtct
     3361 cctggttccg gacaagtttc actgcggtct tgggtgaaag ttcgatgagt agtttcactg
     3421 tctcggaagc ctttcctttt gcagcagcct ctatatacct gccaaggagg atgagggtga
     3481 tgatcatggc agacgtgtca aaatacacct cgccaccaga gaagatttca taaatgctgt
     3541 agatgaaggc cgagccagcg ccgatgatga tgagggaatc catggtgaag cgcaggtgtt
     3601 tgaagccatt tatggtactc ctgacaaaag gcataccaga atagaagagc acagggatgg
     3661 tgagtagcat tgcgatcaat tccagtacat gctttgtgcc gggatcaata ccctggaaat
     3721 atccggcata gagggcgata ctgtaaatca tcagctggga agagaggaag cacgcagttc
     3781 cgaggcgtac caggaggtct tttgtctctg cctgttgaac cctgaactgc tcagattcag
     3841 aaaatggttt tgggatgtat cctatggaca gtatccgctt tagtattctt tcgagaccaa
     3901 tacggttcgg gtcccacctg atctttgccc tgtgcgtcgc atagttcacc cgcgcatact
     3961 caacaccctc ggtcttcaca aggatctttt cattcagcca gacgcaggag gcgcaacgga
     4021 ttccctcgat gtacaagtct atttccctgt cattgcccga ttcaatgaca tactccgcaa
     4081 aaggcttgat gtcaggttct ctctgaaaga gggatgacga tatgccgcgc tcattccatt
     4141 ttctcttctc gtaaaacgcg tccagcccct cctggtgtat caaacggaag atcccgctgc
     4201 agccccaaca gcaaaagact ttctcctgac catcaattgt gtcatggaca gcttcgcggt
     4261 cagggaaatc gagaaggcag tggttacact gaagcatgat atatgaaact ccttgcgagg
     4321 aagtagacgc cggtaatgat taccataaca ccgcttgtca ggtagatcca tcccctgatt
     4381 ctgacgctga ggtaagtcat tgcaagccca aagagcagca gggaagggat ggttcccacg
     4441 ccgaaacaga ggacaaagaa cattgccgaa ggcatactac cggtcgcggc tgcgccgatg
     4501 aacacagaat aggagagacc gcagggaagg aaaccaagga gcagtccgag gggaaaataa
     4561 cgccagagtg aatcaccctc tcttacattc ttcaccatgt gtaagataag gctgttatgt
     4621 ttttcgatga ggcgtatgtt tttaaaaacc cctgtaatgc ctaaccccat cagcaccatg
     4681 aaggcaccgg ccagtatccc caccgcactt tggatgcctg agagctttgt cgcggtattc
     4741 acaaatgacc cggagatacc catgagcgcg ccgataaata cataggtagt gatccttccc
     4801 gcattataca ggagatgggg gagaagcgca gaaaaccgcc ttaagctatc ttttccataa
     4861 agtgtatagg atgctaccag aggtccgcac atgccaatac agtggccaag gcctccgagc
     4921 agtcctgtga agaacgcgag tatatatcct gtattcacta ttcctttacc tcgaaggtgt
     4981 actctgcact cacagtcccg gaggggacgg tatttgtctc gttgatgacg aacgcggctt
     5041 tccatatccg cctgccactg ggacagcgag gaataatgct gctcccttca tatcgtcccc
     5101 ctcccttatg gctcatgatg actttgcgtt cgcccatata catgccaggc atagtcaggt
     5161 ggagaatgat atccgcgtca cttatcggcc tgccccattt gctgagggaa agattaaaat
     5221 aaagaccgga catcattttc acaggttttg gttggatgtc aaaggcagct gttatgtttc
     5281 cgggcatgat tgtcttcaca cagggatctt tgtctatcct gcagtctgtg cccgcggcat
     5341 catgcggctt ggaacagccc agggtgagca gcatcgccca tacccccact atagttactg
     5401 caagagaacg cttcattgct caacgaatat cttctgcggg aagaggaggt tctgtccgtt
     5461 tcgcacaata gtaatccgga gatcccagtg accataaagc ggcagtgcaa caggggcact
     5521 atacacgcct ccttctcctc cactgaacct gtaggtcctg tcatacgcac tacttgaagg
     5581 cctgctgaca gttaacatga gtatggcatc aggaactggt ttttgctccc ggtcagtcac
     5641 ctgaatgagc agatcatttt cccctactgt gaaggtctta ttcttaattt cgatattcag
     5701 gccggattct gtccgttcct tctgctccct gtcccacgac agtccccttt cataaggctt
     5761 atcaacaaca atgccttcaa agcttttgtt gcccacaatc actgccgtga cgaccgctgc
     5821 aagtgccacc atgattacta ttcctatgag gatcttcatt gagtttttgg aattgtgaaa
     5881 cggacacctt tctttattat gaccctgtca gggtcttcgg attcaaggag aatctcgatg
     5941 tcttcagagg tggccccagg aaatatctga cctaccgtga catatacagg gactttttta
     6001 tattcacctg cgtcgagatg caccctctca ggtgtgatac tgatgcttcc ttttggcctc
     6061 ctcacctgca aagtgacctc tttcccggtc cgcgacttat tctcgagaga gaggatataa
     6121 gagtttacca cctttgcgtc agtgaggacg cggggcggaa aggcataatg gggcagaacc
     6181 atcatatcca ggggtgcacg ggatatggac agatagagga agaggaaaag gaaaacaatg
     6241 gccagtgaag tggtcaatac cacgctctga cgtaacaggc gcggcctttg tcccggagag
     6301 ctgaaaaaat aatctataag gccttttttc tgcctgcggc tcatcatcct ggcacaggcg
     6361 tcaatacact cggcacagct gacgcaggcg gcattcgttc cctccctgat atcgatcttc
     6421 acaggacagg tcgtgacgca tgccatgcag ttcatgcact cttcctgcct cctctggtca
     6481 aatgagatga cgagcgttct cctgtcaaag aggacgctct ggagttttgc atagggacac
     6541 acggtcgcgc agaaggtctg tctgagcagg agcaggttta agaatagtat gatggtcatc
     6601 acacaccaga agccgccgat gacgtttcca agagtgccgg agtacagcct gtggaaaaac
     6661 tcatagggag acacaaaata ccatatcagg tttgctgcta cgatcaggct tatgaggaaa
     6721 gtgactgcat aagccatgag tctgtatgtg attcctttgc tgtaggtttt atcgaggagc
     6781 cgggtaaaat ccacgagtac tgtctgtggg caggtccagc cgcaccatat cctgccgaac
     6841 atgagggtga caaggacagt gaaaaaggtc acaaagatca gggcgatgag cacaagaaaa
     6901 aattcatcca tccagaggct taccccgaac acgtggagcc tcagggaagg gatatcaaat
     6961 ctgagggcac tttcaccatt gatggtgaga aagggaattc cgataataag gacagcctgg
     7021 agaatttcag cggttctcct caggggctgg atgcgtttaa tcgtcgtcat ccatcatttt
     7081 gtattttgcc tcttcgacct ttttatgcct ttttttcgaa taataaaaaa cgattattat
     7141 gccgaaaagt atgacgagta caagaccgaa cagaatgtac gcgatggatt gtggagtcat
     7201 ctcctgttta taccttcatt gtttatttct tgtttttcat cataacaaaa aggaccagta
     7261 tcgctacagt tgctgccaag gtgaacccaa tggtcgctac gatcgatata tccatactaa
     7321 tctccttgat gtgcgtatca tgggcagtgc cggtgtcctt gagggaggat acccggcact
     7381 gcccatacga tggctatttt tttacagact cctcatacgc cttctcctgg ctccatccgc
     7441 taatagacgg cgtgtacgag acaaaataat atatgcccca gaggatgaga ccaaaaaaaa
     7501 ggataagcca tccgatgggg atcttacgtt ttgcgtcaac actttcctct acgagaaatt
     7561 cactcagctc atcaagttta tcgtccattg acattgttcc ctcctacttt ccctgtaaag
     7621 acctgatata ggccaccaca gaccagatcc tgtctttatc gagcgttcct ttaaacggcg
     7681 gcatgccgcc ctttgctgcc cggccatctt ctatcatacc gggttgtgtg ccgttgtgga
     7741 tgacctcgaa atagtcatca tcaggcacgt caccggcaac ataaagaaat tcattatcca
     7801 caaggctcgg gcctatgcta cctttggcat tttccccatg gcacaccgca caattggcct
     7861 tgaagatttc ctttccgagc gcgattgcct tggggtcacc tgcaaggggg tttggtatgt
     7921 gagtttcttt gactgctggc ccggcaacag cctcgggctt tttcttgacc gaggtgccga
     7981 tcacctgcat ataggcaacg agcgcatcca tctctgttct cttgcccaat gccgtgatgt
     8041 ctgcaggggc atagggaagt cccaatgcct tcatatgtgc ctcaatgtct gatgggtcga
     8101 gcttgttgtt tcttagccat ccgtaagccg gcatattgga ctctgcaaag aatgcctggg
     8161 gattttcgaa atggccgtaa tgccacgcat cagggtactt ttccccaatc ctcgcaagat
     8221 caggacctgt tctcttggac ccccagagga acggacggtc ataagcaaac tcgccggcct
     8281 tggaatattc accgtatctc atcacctctg tcttgagagg cctgacagtc tgtgtatgac
     8341 agttgttgca gccctcacgc tgataaatat ctttgcccgc gagctggagt gcggcatagg
     8401 gtttcaaatt ttcgagctta gggtgcatct gttgagtgag cattgggaaa aacatggtca
     8461 cgaccgtccc cacaaggatc gcgacagtcg ccagcacagc gaacagaata gggcttgtat
     8521 gcagcttatg atggagttca ttcgccattg cgcacccccc tatgcctttg ccgctgccag
     8581 ggccttgccc ttgcgcatcg tcatgtagat gttgtagatg aaaaagagca tccctatggt
     8641 aaagatcagc cctgcaactg tccttaattt ccagaagggg taattcctta ccagggtctc
     8701 gataaaggtg tatttgagac tgccgtcagg ggtgaccgct ttccagagtg tgccctgctg
     8761 aattcctgtg atccacatgg tgattgagaa catcaattgg ccgatcagca cgagccagaa
     8821 atgggtattt gcgatcttga tgctataaat ctcggtatcg tagatcttcg gaatgatgta
     8881 atagatagaa gctgcaacgg tcatagttac ccagcccata gtccccatat gcacatgacc
     8941 ggggacccag tcggtgtaat ggataagctg gctcacgacc ctcaacccct gagtagggcc
     9001 ctgtacggtc tggagtccgt agaaggtgat gccaaggatg aaaaacttgg tcaggtagtt
     9061 tgtctgcatc ttggaccagt cgctccctac cgtgtagtag ccgttcacca cagagcccca
     9121 tgaaggcgcg ataaggaata tggtgaacac tattgcgaga gtctgtatcc agtcgggaag
     9181 gggggtgtac acaagatggt gagcccctgt ccagaggtac gcaaagacca gggaccagaa
     9241 tgcgataata gagagcctgt ggctgtatat gggaattccg gtggctttgg gaagaaaata
     9301 atagaacatc gcgagtatgg gtgtggtgaa tataaagccc acggcattat gaccatacca
     9361 ccattccaca ttcgcgctgt tcacccctgc aaagagatga taggacttaa acaatccggc
     9421 agggatagag agattgttga ctatgtaaag caccgcaacg gcgatcactg ttgcgatgat
     9481 ataccagagc gatacataca tctgctcctc tttccttttg atgattgtcc cgaagatatt
     9541 caccgcaaat atgacccaga ggataacgac aacgatatca agcggccatt ccagctctgc
     9601 atactcaagt gactggttca ttcctgcaaa gagactcagg gcagcaagtg tgatggctac
     9661 attgaacaga taaagatgaa aacgcgccag ctttgggaaa agcaggggcc ttctggtgag
     9721 ccgcatcacg acataatagg aaatgccaaa aattgcgcct attcccaaac caaaggcaag
     9781 accattggtg tgcacaactc tcattcttcc aaaggtgaag aagggaggga tattccactc
     9841 aggcttccac atctggacag agatccagag gccgatgagt atcccgacca caccccaaaa
     9901 gatcgaggag acgataaaac cctttaccgt ctgattatca tagtgataat cactcataaa
     9961 actcctcctt aatgattatt gcggactctt acagagtcct ttcttgcatt ataggccctt
    10021 tgttttcttt ttaaagaggt ggaggaactc aatcccctga agttagtttt tcttaaggag
    10081 ttctccacca gctttcgcag cttgacccac acaggatgaa cagtacaggt gccgctaagg
    10141 ttgcagatcg aagcgtccac cgcacaccgg ttcatggcag ccggtccttc tatcgcctca
    10201 atgatatcaa gcaggcttat ctgctgaggc ttctttgcaa gccgaaatcc tccctttacc
    10261 cccctgaatg acgttacgac acccgctttg gtaagctttt ggaggatctt tgcgaggaaa
    10321 cttttaggga tggacattgc ccgcgatatc tcgtccgcca taataacggc ttcctttgtt
    10381 cccgaaaggt agagtacgca ccgtatcgca tagtcggttt cacgggtgat ttccatatac
    10441 aggacaaata ttatcctgtt aaggacagca tgtcaagcat tttgtgaggg tagagcagag
    10501 tgaatagtgc gagctagcaa aaaacgatag ttcttattaa aaacttgttg cgtaaggctg
    10561 cggccgatag aatacgatac aatataggtc aggggtgaag catggttcta ttctatgtaa
    10621 aggggttgat gcgatgcatg gattaaactt cgaaactcac ggcagtccgg tttatagact
    10681 ttatgacaga gaaggaacag aaatcgcgta tgacgaggca atagaccgtc tcgcaggggc
    10741 agaagtggtt ttgtttggtg aattgcatga caataccctc attcaccaga cacagatttc
    10801 gatgacagaa gatctctata aaaaaagaaa gggtgagttg atcctgggcg cagaaatgtt
    10861 cgaggccgat aatcagctta tccttgatga atatcttgcc ggattgatcc tgcacaggca
    10921 ccttgttgct gaggccaagg tctgggacaa ctatgagacg gattaccgcg ttctggtgga
    10981 ttttgcaaag aagcacgggc ttaaattcat tgcaacaagt attccgagga gatatgccgg
    11041 tctcgtatca cgcgagggta ttaaggcatt ggacggtctg tccggagaag cgaaacagta
    11101 tattgcgccc ttgccgatag ctgttgctct cgcgactcct ggctaccggg aaatgatgga
    11161 gatgaacctt gatatgggac acgggatgca gatgaatccg gaaaatcttg tggcagcaca
    11221 ggcggtaaag gatgctacaa tggcgtattt tattttgaaa aaccgtgcgt caaagcaact
    11281 ttttcttcat ttcaatgggg tatatcattc ccagaattac ggaggaattt actggtatct
    11341 gaagaaggtg gctccggaac tcaatgtgct cgctctctgc gcagtagaag aggagagctt
    11401 tgagtttcaa gatgaataca aggggctggg ggatttcatt cttgtggtgc ctgctgcagg
    11461 acccccccct ccacgcacgt agccatttct cctttaaatc tttattcttt tggtcttcaa
    11521 tatttgtcag taagttggta agcccattat gctcaagttc ttccgcactc cacacttctc
    11581 atatgtttct cattacttaa actttctaat ggaatcaata agataaggcc tcctcttgtc
    11641 tccatttttt gtcgttgaca aaggcatcgt tgtgtgcctt aattaataag gccagttcat
    11701 gaatttataa ttttttttaa tttgctttgt ccacctattc tttgatatct ttccggaaga
    11761 aacaggacag gagtcggcgg gtcttgcaaa gatggccgag ttagttaagg gtagtaaccc
    11821 taggctcaac tcggcctttt tatttctcta attgaggcaa cgggaaaagg aggaagattt
    11881 gtatgaaaag ggtttggtta ttgattgcaa tgttgttgtt ggccggttgc ggcagtggtg
    11941 gaggtagtaa tgtctccaat tctcagccgg ggggaagtca gccggggggc attattgagg
    12001 ttgggccttc gacgccgacg ggaaatgttg tcgtgaatat cgtcgagggc gatgcaaaaa
    12061 catccgctgc cataccacct gatgaccatg tacggctcgt ggttaccaat cccaacctta
    12121 gaattaacgg ttctgtttac aagctgattg tggatggcct taaccccggt ggtcatataa
    12181 ccggtctcaa atttccggta gcaaccggtt atacttttga actaataacg tatgcgcctg
    12241 accatgttca accattgggt accgtaccaa tcaacagaat ggtgcaacat gcaaaaacac
    12301 cagtccccgt gaacatcacg ccggatggcg ccacggtgga cttaaccata aacactataa
    12361 ctgcttgcat caatgacata cctactccgg cctattccaa tgccccgctc ggtataactg
    12421 ctacttttac attgcagccg actccgctgc aaccaggttg gaacttattt cttaacacat
    12481 ctttaccctt gatgagtcat gccctgcact ccacagccac tccgatagcg aatcacgcgc
    12541 tggtcaaggc gcctatagta ttaaccgagc aaacattgta tgcacaggct gagttttaca
    12601 tcaatcagtc cttgctcgac acaaccggat tgttggacat acttaacccc attacaatgg
    12661 cttctcctcc cacattagca gccccccacg cggctgaaaa tattcatgac tggactttca
    12721 actgtcccaa tcctgatgct ggcgatgatc caagtgctca ttctgtattt ttgcagttag
    12781 tcggtgtttc tattccaact ccttaaactg aactaaacaa aaaaccag
//
LOCUS       DATKOU010000008        17791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1551676, whole genome shotgun sequence.
ACCESSION   DATKOU010000008 DATKOU010000000
VERSION     DATKOU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 17791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17791
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551676"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /gene="tsaD"
                     /locus_tag="VMU21_00240"
     CDS             complement(<1..941)
                     /gene="tsaD"
                     /locus_tag="VMU21_00240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUO75979.1"
                     /translation="MVILGIDTSCDDTSASVVEEGVRIRSNIVSSQEIIHGRFGGIVP
                     ELASRQHVQMIWPVVDEALRSAHRTLEDLSAVAVCHGPGLIGSLLVGCSFAKALCFSK
                     GIPLVSVNHLEGHVFSSFLENSSYPLEFPFISLIVSGGHTSLYRVDGFGKYRELGRTR
                     DDAAGEAYDKVSKLLGLGYPGGPVIDRLACEGSPDAVVFPRAYLPEGLDFSFSGLKTA
                     VMNHLKEPHPAGINDIAASFQQAVIDVLVRKTEWAITRERVHRVTVSGGVGANSALRR
                     ELALRAKEKGIQLFIPAASLCTDNAAMIAAAGYHHVKA"
     gene            complement(962..1621)
                     /locus_tag="VMU21_00245"
     CDS             complement(962..1621)
                     /locus_tag="VMU21_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HUO75980.1"
                     /translation="MIARAVQRGFFLTLCCFVLLCTDASRTQGGDIPGEKIQKAYEDI
                     KDITGEFVQKSHIKDLKRTDTFKGTFVIKMPSQMKWQYFDEKKRHFTEVLIAHDELLI
                     YQKEEKQVFKGKFDSESYGQAPIALLTGFAHIDREFTVAEKNGKLLLKPRKGMGSVVS
                     IEITPSDGEFPIDSLTIIDRRSNTISITLKDVRINSGVKEAAFSFSLPQGVSVYQYRP
                     Q"
     gene            complement(1618..2040)
                     /locus_tag="VMU21_00250"
     CDS             complement(1618..2040)
                     /locus_tag="VMU21_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75981.1"
                     /translation="MDRQKVVLRFVDGRLIKGHLDDFSPKADFVLLEEESGARQRFGV
                     DELKAIFFVRTFEGDKTRSEKKSFEKENPPGKRVFVKFKDAESMVGYVEGGVPWEKGF
                     FLEPAKGKGFFLVPADDASNNTKVFVIAQSVWEVTVMG"
     gene            2168..3103
                     /locus_tag="VMU21_00255"
     CDS             2168..3103
                     /locus_tag="VMU21_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75982.1"
                     /translation="MRAAAIDVGSNTLRMLIGDIHEYGITRISFERAITRLAQGMTNS
                     RVLSEKNMKESLLVLRGFSQSLARYGITHVQAVGTSALREAKNGQGFADAVFCETGIR
                     IEIISGIREAELTLGGAMLGMEAEGVSLIIDIGGGSTEWIISLSGSLQSSLYGSMPLG
                     VVTLCERFVSGDPPSHREVSALSAEINVHLHSLRQKLPGRTVPITRLVGTGGSITTLA
                     SIDLGLETYNHEKIHTHLISLLRLYALREKLLSLPLSARKTITGLEPERADLIIPGIL
                     LTIKWMELFEAREIVVSDFGLLEGLLREACDETRL"
     gene            3087..3836
                     /locus_tag="VMU21_00260"
     CDS             3087..3836
                     /locus_tag="VMU21_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUO75983.1"
                     /translation="MKPAFERPKSLKSAPFRYCPGCGHSLIHRLIAESIDTLGIRERV
                     IGIAPVGCAVFAYDYFDFDILEVAHGRPPAAATAMKRVLPDRIIFSYQGDGDLAAIGT
                     AEIIHAAARGENITVFFINNATYGMTGGQMAPTTLVGQKTTTTPGGRDSRQSGYPLRV
                     AEMLATIDGASLIARTSVDSPRNLLRTKTLIEKAFRYQREGKGFTLLEILSPCPTDWG
                     LSPQESVKWIQSDMIPVYPLGVLKDRYAEEA"
     gene            3833..4435
                     /locus_tag="VMU21_00265"
     CDS             3833..4435
                     /locus_tag="VMU21_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HUO75984.1"
                     /translation="MKSVGILVAGFGGQGILLLGRLIAYSGMLEGKEVTCFPSYGAEM
                     RGGTANSTVIVSDEMIGSPIMGNPDILIAMNEASLKRFQPKIKSGGVLIFDSSLISSP
                     EIRGDIHVIHIPATEIAASIAESEAPNKNPVLTATRVRSANMVMFGALLAKTGIVKVE
                     TAIHALERLTSSKREKTLKENKKAVMRGVAYIASKKSKDN"
     gene            4407..4862
                     /locus_tag="VMU21_00270"
     CDS             4407..4862
                     /locus_tag="VMU21_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HUO75985.1"
                     /translation="MPVRKAKITDAKAIHRLVNEFAKKGEMIPRSLNDLYENIRDIYV
                     YEERGEIKGVCALHILWEDLAEVRSLSVRLDAQGFGIGKKLVTTCIREAKTLGIKRIF
                     ALTYHPEFFEKIGFGVIDKSKLPQKIWGECLRCPKFPECDESAVIMNLS"
     gene            complement(4857..5417)
                     /locus_tag="VMU21_00275"
     CDS             complement(4857..5417)
                     /locus_tag="VMU21_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HUO75986.1"
                     /translation="MRNRQSGASKALSERINRLYIVLLLFTFHFSLFTVSSAELIDRV
                     VAYIDDKAITLSELNETFDRTRKVQPDITRREVLDTMVNRMLLLNEAKRLRFEGKSDE
                     ELLNEYIELKVKAFIRLREEDIEDYYQKNIDEFKEAPYESVRDKIEHYLTERETNSLL
                     KKQIEELRAKAYIKILIREQGDGGGS"
     gene            complement(5419..8016)
                     /gene="bamA"
                     /locus_tag="VMU21_00280"
     CDS             complement(5419..8016)
                     /gene="bamA"
                     /locus_tag="VMU21_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HUO75987.1"
                     /translation="MKTIEVEGLSSLSAGELIELLDVKAGEVLNPSQVRAGIKRAFLK
                     GIFDDIEVYSEEGGARIRVEVKEKDTIRKVRTTGNEYFSDREIRNMFLMKEGQVLRYD
                     LLDEAVKQLKAELSERGFPDAIVSVSASSVSKPFEKDLTIMVREGKPLLIERIVVRGA
                     PEDEIKSMMYINEGEVYNQEAIKKDVKRIVTRYKKHGYLNPAVSYRFTEGRFEVDVTK
                     GKKLVVQFEGNAVFSAKRLMKEMPFLEAGEVRDDLVEDAARKIVSLYYSKGYPFAQVA
                     PVISNTAEASGDTIEVKFFVFEGEQVTVGSLDFTGLTLPAKKLKDLMPLKEGSDYNPD
                     LVSTDVDVVREFCVALGYLSAEVDEPKVSIEQNKASITISVKEGPRTLLHHIEVSGAV
                     SVPLGEIQPLISLKQGDPYNEVDIADTRARITDLYHERGFTDATVSAKVEMGGEGASV
                     TFEIQEGKKTFFGKTLITGNTRTQREVIERQLLHKEGEPFNSGVLVKERQKLYKLGLF
                     SDVRIERLDTFGQTGDIRIDVVEANAGTVDFGFGYSNYEKFTAFVDLGYKNLLGMNKQ
                     ASLRIGYNSLEKLYEINYLDPWFFSGDLHLKGTLFYIQKEEKNIDTDVTMYRYKKTGG
                     SLGIEKQYGNTTKGELSYEYVRAYTYDVQPDMILTPQDTGHLTIGSIRTGVSYDTRDN
                     PFNPRKGLLGGVTMQVAASALGSQTSFGKVIFNGSLYQELSKPFVLAVAFRFGLAQAW
                     DSSSEILPLIERFFLGGRSTVRGYAQDTVGPLGSDNNPTGGNAFIETNVELRTSVWKG
                     LGIVTFLDSGNVWQKIGDIDWSLKHTVGAGLRYDTPVGPFRLDYGYKLKRVTGLSRSE
                     LFFSIGQAF"
     gene            complement(8142..11618)
                     /locus_tag="VMU21_00285"
     CDS             complement(8142..11618)
                     /locus_tag="VMU21_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HUO75988.1"
                     /translation="MITCSSSQVETIIENIKKYLEEERKTPLTVKVKAVALDNGRFTL
                     QYKENTFRASGVSGEAILNAWENFIAQRHAPPKISFSLRELSSTIKGWPELKAEVKGT
                     VVLKDGQLDVKGLQVGLYGSLINASGIFSSEGSKSLPKNSGDFHLRAGLLPESFKKIF
                     GLKRQGEGEIYASGTVRLTMDDLQQSVMDMELKGDFYMQTLMELLKVKERVEGFVDFS
                     GTLKGPLNRLTGTAKARLKKGNLFDIDVDELRCSVLYGDRSLRFLEGKADLYHGHAEA
                     ELTLSVSGDEYYALKTKFSDVDSPAALTLIGWKPDIPFGKVKGEVSTSGTSFNPSGWY
                     VYEASGQGKDVLGRIRKIKGSFVSRNNVFTLSDSEASTEKSVFGFYGDVDINTTQLRL
                     TIQGKTGDLTDVTLPYRRELTGRGEFSGTVTGSFDNPLIAGRMKLYSAAYEEYSLGDI
                     AGDVNYRRDLLEVKQLTATLNPQQGQAVTAVLKGTVRFPESKEFFDLKRPVYGLSVAV
                     YNADLETFVKSVYKKPLKSYPKGTFDTAISIGGTEPAPLFKGTARIQSLNLYGVVADS
                     LSLSFSYDYRTLGAEGVLSGSRMKGLDLGGGKLRASMKDDILVLDGALFNDRVTLDGR
                     ANMKGVKPWTARLEVKSGRYDFLVAPFLKDVPEDLLINMKGYAEMSGDNNHFTAKAFL
                     NQLSIALYGYNFSNESEIRFELKDKTLALTSVKMRSGSTSFQVTGDCEIGKEYNLVME
                     GNSTLLPLKSFSKKIDTLKGDAGFVFGVTGKWDAPKINGGVTVSNAIFGVRDIPSKVS
                     SINGYFYMDGDKIVIQKLSGKTGGGDVELSGAAFLKGFALKRFYIDAALSNIGVNVSR
                     EFPVNFSGNLFYTGTLDSQTLSGDIRINHAFYREPLEWKSWVLKGKAPERPRGEIGAF
                     EKIGLNIRVLGSDNIVINNNMARASLKLDLVLRGSVANPLIFGRAETRTGIVYFRNNE
                     FKILSATADFADPKRINPILNILAETTISNYDIRMNLNGQIEHFDMTLSSTPSLEETQ
                     ILSLLTLGTLGTASTGIQGGINLSAATSFLSGQLQDIAQERVKSITGIDRIGTESYTS
                     RVTGKSEQRFMVSKRLIGDKLSVTYATSFNAVETSVIRIEYNVGGNVTLIGLKDESGG
                     LGGNIKFRFSFK"
     gene            complement(11625..11945)
                     /locus_tag="VMU21_00290"
     CDS             complement(11625..11945)
                     /locus_tag="VMU21_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75989.1"
                     /translation="MGKITVKKILFVAVPVVIVVLTMVFFRGPHISNLLKNLILPELS
                     SLTGKQVMAQKITLNLFPLFIEAKGIKVLDEGIEIVHLPRVKGYIEASGLLRKRLVLR
                     RITV"
     gene            complement(11927..14512)
                     /gene="glnD"
                     /locus_tag="VMU21_00295"
     CDS             complement(11927..14512)
                     /gene="glnD"
                     /locus_tag="VMU21_00295"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HUO75990.1"
                     /translation="MDIHELSELIKQGYSGRQLTTLFTGRTDSLLKNLFSSMNAHSGL
                     CLMAVGGYGRGELAPFSDIDIMLFARDRSASGKASEFLYKLWDTNRAIGHSFRTPPDC
                     ISEARKDVKTRTSLLEHRYIAGDRDLYQYFMDTVYPEIAFRETGRFITEKLREIEMRH
                     RNISDSVFMLEPHVKEGRGGLRDIHTLVWLASIKLRIRSFDELGKILSPEEFKRLANA
                     YDLLLKARFCLHVLSGRRNDILSFEYHDGIAEMLNFKASKRFLSSERFMRYFYLKASA
                     INDITSRSLDLFSMPRTGREERDSRILPFLYAKKRITDDFSLSKNRIVANGDFLKERP
                     ESIIEAFSVLAKTGKRFSPLLREEIGKNLHRIGKTTRSSQKAVESFMNIIRGDRTYET
                     LREMHNSRVLGRLIPEFGALSFLVVYEPYHRYTVDEHALYAVKKLVELRDTKYKNLEH
                     LSAVFREVRHKEALIFSLLLHDIGKKGIVKSYRYGSGGGHHEEAGYLEVKNVLEKFNL
                     TVGLRTRIEFLVKNHTLMSSVAFKGDTDDIEVIAQFADDVGDRENLDALYLLTYADIA
                     SVGPDFWTEWKAYLLRELYEITSQYLSGFVGVLPGSQGFSGVRGNHPLSRTMFRSDNE
                     RVGTERFLALMPERYIISSTPERVSGDYALYREVTGKGFGVRVKEDPAGTAEIIVGAW
                     DRPGLFSRIVGVLSSLGMNIYRARVYTGSGGIVIDKIQIANWGDICWDGLCQDLESRL
                     REAVCSGEGEKWRRLGIQENRVLPGCSDVKPEVMGRFGPFIEIDNETSRESSTLEFFA
                     QDRLGLLYDVTSLIHEKDIDIISARINTESGIANDILSLQQRGKKLEGMTVHELLLSL
                     WERLQ"
     gene            complement(14607..14930)
                     /locus_tag="VMU21_00300"
     CDS             complement(14607..14930)
                     /locus_tag="VMU21_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75991.1"
                     /translation="MKKDKAKPLKTRRSKLKHLNAFLKEYQPIINALIKTLGFLVLLL
                     ASLRSCHDNRQVIENRMETDEREECVIESIHFHGIIEKVGLCPYVCPDMGKSNVKEQP
                     RGDCP"
     gene            complement(15397..16236)
                     /locus_tag="VMU21_00305"
     CDS             complement(15397..16236)
                     /locus_tag="VMU21_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO75992.1"
                     /translation="MEIEKQIKEYLRCLDESLDILSLHKEGELIGLPSWALSTRTITI
                     GVAGNDGIVLKVVPDKALDDDVVNILEIKSVEQIRDLFAQLGYDGPPIERLDENFFAL
                     WGDTVMYSADNPLLPPALDNKCLLGLGRKKGFQSIFNVENAKQDAIDFWNKRRLPKTH
                     SSYVVELKTILGKFHALIKRKAFLERRIHRFIYEHKNVLLPPHKRCFFEHTLYYGTDS
                     CKADFVLEREQGLSSLLIELESPVHAVFTKTMDLTAPANHARQQISKWVLFIDSDAVR
                     NAS"
     gene            complement(16343..>17791)
                     /locus_tag="VMU21_00310"
     CDS             complement(16343..>17791)
                     /locus_tag="VMU21_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HUO75993.1"
                     /translation="ALEILANALAYLPKDEPVSFIEPGLGTGVFFSALRRIANSRTLK
                     EAVGVELDPTYAEISRAIWGQYGLTVEQIDFISFSRSPEQANRYNLLCTNPPYVRHHH
                     INPETKRSLQTSLDRELGISVSGLSGLYVYFILLAHSILANNAVASWLVPSEFLYVNY
                     GKALRDYFRHHVTLLNIHQFDAEEVQFDDALVSSCVVTYMKRLPSPDATFRFSFGADM
                     LNPKHSQEIALSNGTLNGKWTVLHLKDEAERDDEEYEIGDFFTIKRGLATGANEFFIL
                     DKGIIDHYSIPAGFLKPILPSPRYLKDTVIQSEADGSPRIDKVKYLLDCDVPPEIVKE
                     RYSGLWNYFKRGVEKNIPQRYLCAGREEWYYQERRTPSPFLATYIGRSQNGTKSPFRF
                     LLNLSNALVTNVYLNLYPNELLSALLREDKARFVEMLELLNGISIEEVLRAGRSYGGG
                     LHKIEPKELAGVPLRKIPEWLKAGAAKQLVML"
BASE COUNT         4397 a         4745 c         3919 g         4730 t
ORIGIN      
        1 gccttcacgt gatggtatcc ggctgcggca atcattgccg cattatctgt acagagagaa
       61 gcggcaggaa taaataattg gatccccttt tcttttgccc tgagcgctaa ttccctcctt
      121 agtgcactat ttgctcccac accgccggat accgtcactc gatgcacccg ctccctggtt
      181 attgcccatt cagttttcct caccaataca tcaattaccg cctgctgaaa actcgcggca
      241 atatcattga tacccgcggg atggggctct ttcaagtgat tcatgactgc agtcttaagg
      301 ccactgaaac tgaagtcaag gccctccggc agatatgccc tgggaaaaac tactgcgtca
      361 ggacttccct cacaggccaa tctatcgatt acagggcctc cggggtatcc gagaccgagg
      421 agcttcgata ccttatcata cgcctctccg gcagcgtcat cccttgtcct gccaagttcc
      481 ctgtattttc caaagccgtc aacacgatag agactcgtat gcccccctga cacgataagg
      541 gatatgaagg gaaactcaag ggggtatgaa gaattctcca aaaaacttga aaatacatgg
      601 ccttcaagat ggtttaccga gacaaggggg atacccttcg aaaagcacag ggcctttgca
      661 aacgaacacc ccacgagcag ggaacctatc aatccgggtc catgacacac cgcaacggct
      721 gagagatcct caagggttct gtgagcggac ctcaaggcct catccaccac aggccatatc
      781 atctggacat gctggcgtga tgccagttca ggaactatgc ctccaaacct gccgtggatg
      841 atctcctggc tggaaacaat attcgagcgt atccttactc cctcctcaac cacggaggcg
      901 gatgtgtcat cacatgaggt gtcgataccg aggatcacca taggggttcc tgcgctcttc
      961 tttactgcgg ccggtattgg tagacgctca ctccctgggg cagggaaaaa gaaaatgcgg
     1021 cctccttcac ccccgaattg atacgcacat ccttaagggt gatacttatg gtgtttgagc
     1081 gcctgtcgat gattgttaag gaatctatgg ggaattcccc gtcagaaggg gttatctcta
     1141 ttgagacaac gctgcccatg cccttccgtg gcttaaggag taacttcccg ttcttttcag
     1201 caacggtaaa ttccctgtca atgtgcgcaa accctgtcaa gagggcaatg ggagcctggc
     1261 cgtaactttc actgtcaaat ttccctttaa acacctgttt ttcttccttt tggtaaatga
     1321 gcagctcatc atgggcgatg agcacttcgg tgaaatgccg tttcttttca tcaaaatatt
     1381 gccatttcat ttgcgagggc atcttgatca caaacgtgcc cttaaacgta tctgtcctct
     1441 tcaggtcctt gatatggctc ttctgaacaa actcccccgt gatgtctttt atatcctcat
     1501 atgccttctg gatcttctcc cctggaatgt ccccaccctg ggtccgggaa gcgtctgtac
     1561 atagaagaac gaagcagcaa agagtcaaaa aaaaacctcg ctgtaccgcc ctcgctatca
     1621 tcccatcacc gttacttccc agactgactg tgcaatgacg aacaccttcg tgttattact
     1681 cgcgtcgtca gcgggaacaa ggaagaaacc cttacctttt gcgggctcca gaaagaagcc
     1741 cttttcccag ggcaccccgc cctcgacata ccccaccatg gattcggcat ctttgaattt
     1801 cacaaagacc cttttaccgg gagggttctc cttttcaaag gacttcttct cgctcctggt
     1861 tttatcccct tcaaaggtcc ttacgaaaaa tattgccttg agttcatcca ccccgaatct
     1921 ctgtctggcc cccgactctt cttctagtaa aacaaaatcg gccttgggcg aaaaatcatc
     1981 aaggtggcct ttgatgagcc ttccgtcaac aaatctcaac accactttct gtcggtccat
     2041 cgtgaaaatt ttaccatatt acaaaaatat ttattagtat ctttttgccc ttccctcccc
     2101 cagatgaatg gttttcacta tcctgggaaa attgacctct cctcgtatgc taaaatagca
     2161 attcgccatg cgagctgccg ccattgacgt aggctcaaat accctgagaa tgctcatcgg
     2221 ggatatccat gagtacggaa ttacccgtat ctcctttgag cgcgcaatta cgcgacttgc
     2281 tcaaggaatg acgaattccc gcgtgttaag cgaaaaaaat atgaaagaat cccttttggt
     2341 gctgcggggg ttctctcaat cccttgctcg ctacgggata acccatgtgc aggcagtggg
     2401 aaccagtgcc ctgagagagg caaaaaatgg tcaaggcttc gccgacgccg ttttttgtga
     2461 gacaggcata cggatcgaga ttatttcggg catacgggaa gcagaactca ccctcggggg
     2521 cgcgatgctg ggaatggaag cagagggagt ctccctgatc atagatatcg gagggggaag
     2581 caccgaatgg ataatctcac tgagtggatc tcttcaatca tccctctatg gttcaatgcc
     2641 cttaggagta gtaactctct gtgagaggtt tgtgagcgga gatcctcctt cccacaggga
     2701 ggtctcagcc ctctctgccg agatcaacgt gcaccttcac tccctgaggc aaaagcttcc
     2761 aggacgtact gttcctatca cgcgtttggt gggaaccggc ggttccatta ccacacttgc
     2821 atcaatcgat ctcggtctgg aaacctacaa tcacgaaaag atccacacgc atctcatttc
     2881 tctcttaagg ctatatgccc tcagggaaaa gttgctctca ctccctttaa gcgccaggaa
     2941 gaccatcact ggccttgagc ccgagagggc cgacttgatt atccccggca ttctgcttac
     3001 aattaaatgg atggaacttt ttgaagcccg tgaaattgtg gtgagtgatt ttggattatt
     3061 ggaaggatta ttgagggagg catgtgatga aacccgcctt tgaaagaccg aaaagcctta
     3121 aatctgcccc ctttcgctac tgtcccggtt gtggccatag cctcatacac aggctcatcg
     3181 ccgaaagcat cgatacgctt ggtatcaggg aaagggtgat cggcattgct cccgtggggt
     3241 gtgcggtctt tgcatatgac tattttgatt ttgatatcct ggaggttgcc catggcagac
     3301 cacctgccgc tgcaacagct atgaagaggg ttttacccga caggataatc ttttcgtacc
     3361 aaggcgatgg cgacctggcc gccataggga ccgctgagat aatacacgct gcggcaaggg
     3421 gagaaaatat cacggtattc ttcatcaaca atgcgaccta cgggatgacc ggcggacaaa
     3481 tggcacccac caccctcgtg ggccagaaaa cgactaccac gcccgggggg agagactcca
     3541 gacagtcagg gtatcctctc cgtgttgcag aaatgcttgc caccattgac ggggcttcac
     3601 ttattgcaag gacatctgtg gactccccca ggaatcttct caggacaaag actcttattg
     3661 agaaagcgtt cagatatcag cgggagggaa agggattcac tctcctggaa atactttccc
     3721 cgtgcccgac ggattgggga ttatcccctc aggaatcggt caaatggata caatccgata
     3781 tgattccggt ataccctttg ggggttctga aggacagata tgcggaggag gcttgaaaag
     3841 cgtcgggata ctggtcgcag gcttcggagg ccaggggatt ctcctgttgg gaaggcttat
     3901 cgcctacagc ggaatgctgg aagggaagga ggtaacctgt tttccctcct atggtgctga
     3961 gatgcgcgga ggaaccgcca actccacggt aatcgtctct gatgagatga taggctcccc
     4021 tattatgggg aaccctgata tcctcattgc catgaacgag gcatccctca agaggttcca
     4081 gccgaagatt aagtcagggg gagtacttat ttttgactct tctctcattt cctctcccga
     4141 aataaggggg gatattcatg tcatccacat acccgcaacc gagatcgctg cgtccatcgc
     4201 tgagtccgag gcgccaaata aaaacccagt gctcaccgct accagagtaa ggtctgcaaa
     4261 catggtaatg tttggtgcat tgctcgcaaa aacagggata gtgaaagtgg aaaccgcaat
     4321 acatgccttg gagcggctta cttcctcaaa aagagaaaag actcttaaag aaaataagaa
     4381 ggcagtcatg aggggggtcg catacattgc cagtaagaaa agcaaagata actgatgcaa
     4441 aagcaatcca caggctcgtc aatgagtttg caaagaaggg ggaaatgatc ccccggtctt
     4501 tgaatgatct ctacgaaaac ataagggaca tttacgtcta tgaagaacga ggcgaaatca
     4561 aaggggtatg cgccctgcat atcctgtggg aagatctggc cgaggtacgg tccctctctg
     4621 tgcgcttgga tgcgcagggc ttcggcattg ggaaaaagct tgtcaccacg tgcataagag
     4681 aggcaaagac gttggggata aagagaatct ttgccctcac ctaccacccg gaattcttcg
     4741 aaaagatagg attcggtgtc attgacaagt caaaacttcc tcagaagata tggggtgaat
     4801 gcctcagatg tccgaagttt cctgagtgcg acgaatccgc ggtcattatg aatctctcat
     4861 gagcctcctc cgtccccttg ctccctgatc aggatcttta tatatgcctt tgccctgagt
     4921 tcttcaatct gttttttcag aagactgttg gtttcccgtt ccgtgagata gtgttcaatc
     4981 ttatccctca ccgattcata cggggcttcc ttgaactcat cgatgttctt ctgataatag
     5041 tcctcgatgt cttcttccct gagccgtatg aatgccttga ccttcagctc tatgtattca
     5101 ttgaggagtt cctcatcact cttgccctcg aatctcaggc gtttcgcctc attcaatagg
     5161 agcatcctgt ttaccatagt gtcaagcact tcccgcctcg taatgtcagg ctgcaccttt
     5221 ctcgtcctgt caaaggtttc attgagttcg ctgagggtaa tggccttgtc atcaatatag
     5281 gccacgaccc ggtcgatgag ctctgcactt gaaacagtga aaagtgaaaa gtgaaaagtg
     5341 aaaagcaaaa ggacgatata tagcctattg atcctttctg acagcgcttt gctggctcct
     5401 gactgtcgat tcctcatctc agaacgcctg cccgatgctg aagaatagtt cgctcctgct
     5461 gaggccggta accctcttga gcttgtaccc gtagtcaaga cggaacggcc caactggtgt
     5521 gtcgtatcgc aatcccgcac caaccgtatg cttgagagac cagtcgatgt caccgatctt
     5581 ttgccagaca tttccgctat caagaaacgt aacgatgccc aaccctttcc aaacagaggt
     5641 ccgcagttca acgttcgtct cgataaaggc attaccccct gtagggttat tgtctgatcc
     5701 cagcggtccg acggtgtcct gtgcatagcc cctcactgtg ctcctacccc caaggaaaaa
     5761 acgctcaatc agcggaagga tctcggaaga agaatcccat gcctgggcaa gcccgaaccg
     5821 gaacgccacg gcgaggacaa agggcttcga gagttcctga tagaggcttc cattgaatat
     5881 caccttccca aagctcgtct gggagccaag ggcagaggca gccacctgca tggtgacccc
     5941 tccgagaagg cctttcctcg ggttaaacgg gttatccctt gtatcataag agacaccggt
     6001 ccggatacta ccaatagtga ggtgtccggt gtcctgaggc gtaagtatca tgtccggctg
     6061 cacatcatag gtgtacgcac gaacatattc gtaggaaagc tcacccttgg tcgtgttgcc
     6121 gtactgtttt tcaatcccca gagacccccc tgtcttcttg taacgataca tggtcacgtc
     6181 agtgtcgata ttcttttcct ccttttgaat atagaaaagg gtgcccttca ggtgaaggtc
     6241 ccctgaaaaa aaccaggggt ccaggtaatt aatttcatag agcttttcga gagaattgta
     6301 tccgatcctc agggacgcct gtttgttcat cccaaggaga ttcttatagc cgagatccac
     6361 aaaggcggtg aacttctcat aattgctgta cccaaagcca aagtccaccg tgcctgcatt
     6421 tgcttccact acgtcaattc ggatgtctcc cgtctgacca aacgtatcga gacgctcaat
     6481 gcgaacatcc gaaaacagtc cgagcttgta taatttctgc ctctccttta cgagtactcc
     6541 ggaattgaat ggctcccctt ccttgtgaag caactgacgc tcaatcacct ctctctgtgt
     6601 ccttgtattg cctgtaatca atgtctttcc aaaaaaggtt ttcttccctt cctgtatctc
     6661 aaaggtgaca gaggctcctt ccccacccat ttcgaccttt gcactcaccg tggcatcagt
     6721 aaatcccctt tcatgataga ggtcagtaat ccttgcccgg gtgtccgcga tgtcgacctc
     6781 attatagggg tctccctgct tcagactgat caacggttgt atctccccaa gagggacaga
     6841 gactgcgccg ctcacctcaa tgtggtggag aagcgtcctc ggaccctcct tgactgatat
     6901 ggttatactc gccttgtttt gttcaatgga caccttgggt tcatccacct ctgcactgag
     6961 atatcccagc gcgacacaaa attctctcac cacgtccaca tcggtagata cgaggtctgg
     7021 gttatagtca cttccctcct taaggggcat gagatcttta agcttcttcg caggcagcgt
     7081 cagtcccgta aaatccagag aacctaccgt cacctgctca ccctcaaaaa cgaagaattt
     7141 cacctctatg gtatcccctg atgcttctgc agtattggaa ataaccggtg ccacctgggc
     7201 aaatggatat cccttgctgt agtagagcga tactatcttc ctcgccgcgt cttcgacgag
     7261 atcatccctg acctctcctg cctcaagaaa aggcatctcc ttcatgagcc gctttgcgct
     7321 gaacacggcg ttgccttcaa actgtacaac gagcttcttc cctttcgtca catccacttc
     7381 aaacctgccc tcagtgaatc gatacgatac ggcaggatta aggtagccgt gtttcttata
     7441 gcgcgtcacg attcttttta catctttttt tatggcttcc tggttataca cctctccttc
     7501 attgatgtac atcattgact ttatctcgtc ttcaggtgcc ccgcgtacta caatcctttc
     7561 tatgagaagc ggtttccctt cccttaccat gatggtgagg tccttttcga agggctttga
     7621 tacagaggat gcagaaacgg acactatagc gtcggggaac cctctctctg aaagttctgc
     7681 tttcaactgc ttcactgctt cgtcaaggag atcatacctc agcacctgac cttctttcat
     7741 cagaaacatg ttccttatct ctcggtcaga gaagtattcg ttccctgtgg tgcgaacctt
     7801 tcggatagta tccttctcct ttacttcaac ccttatcctt gctcctcctt cctcactgta
     7861 tacctctatg tcatcgaaga tccctttaag gaacgctcgt ttgattccag ccctgacttg
     7921 agagggattg aggacttctc ccgccttgac atccagaagc tcaatgagtt ctcccgcact
     7981 gagggaagag agaccctcca cctcaatcgt cttcacttcc tcaacagacg ttccggcatg
     8041 agcacacgat tcgtgaggaa ataaagggag aagtacagat gtgagaaata ctatgaagga
     8101 tatgaaatag tatgttgagt tcatcctatg tcttcattct cttatttgaa gctgaagcgg
     8161 aatttaatat ttccacccag ccctccggac tcgtccttca agccgataag ggtaacattg
     8221 cctccgacgt tgtactcaat ccttatcacc gaggtttcca ccgcattaaa ggatgtggca
     8281 taggtgaccg agagtttatc tcctataagt ctctttgaaa ccatgaaccg ctgctcggac
     8341 ttccctgtta ctcttgaggt gtacgactca gttccaatcc tgtctatccc ggttatgctc
     8401 ttcacccttt cctgtgcaat atcctgcagc tgtcccgaga gaaatgacgt ggcagcactc
     8461 agattgattc ccccctggat tcccgtggat gccgttccca gggtaccgag agtgaggagg
     8521 ctcagtatct gtgtttcttc aagggacggg gtagaactga gcgtcatatc aaaatgctct
     8581 atctggccat tgagattcat ccgtatgtca taattactga tagtggtctc cgcaagaatg
     8641 ttcagaatcg gatttattct tttcggatct gcgaaatcag cggttgcgct gagtatcttg
     8701 aactcattgt ttctgaaata cacaataccg gtccttgttt ctgcacggcc gaagatcagg
     8761 ggattcgcga cacttcccct cagaaccagg tccagcttga gtgatgccct ggccatgtta
     8821 ttattgatca ctatattgtc agaaccaagg accctaatat ttaaacctat cttttcaaaa
     8881 gccccaatct ccccacgggg tctctccggt gccttccctt tgagaaccca actcttccac
     8941 tcaagaggtt cacggtagaa tgcgtggtta atcctgatgt ctccactcaa tgtctgggag
     9001 tcgagggttc ccgtatagaa aagattcccg ctaaaattca caggaaattc tcttgacaca
     9061 ttcactccga tattactcaa tgccgcatca atgtagaacc ttttcaacgc aaatcccttg
     9121 agaaaggcgg cccctgaaag ctccacatca ccgcctcccg tcttgccaga gagcttctgg
     9181 atcacgattt tgtctccatc catatagaaa tacccattta tggagcttac cttagagggg
     9241 atgtccctca ccccgaaaat ggcattggaa acggtaaccc cgccattgat cttcggggca
     9301 tcccactttc ctgtgacacc aaacacgaat cccgcatctc ccttaagcgt gtcaatcttc
     9361 tttgaaaaac tcttcagagg caagagcgtt gagttcccct ccatgacgag attatactct
     9421 ttccctattt cacaatcccc ggttacctgg aatgaagtgc tcccactcct catcttcaca
     9481 gaggtgagcg ccagcgtttt gtccttcaat tcaaaacgta tctcggactc attggaaaaa
     9541 ttgtagccat agagcgcaat ggataactgg ttcaagaatg ctttggcagt gaaatgattg
     9601 ttgtcccccg acatctcggc atatcccttc atgttaataa gcaggtcttc gggaacatcc
     9661 ttgagaaagg gcgcgacgag gaaatcgtac cttcctgatt tgacttccag acgggcggtc
     9721 cacggtttca ctcctttcat attcgccctt ccgtctaacg taacccggtc attaaaaaga
     9781 gcaccatcaa ggaccaggat atcatccttc atggaggccc tgagcttgcc tcctcccaga
     9841 tccaggccct tcatcctgct cccgctgagg accccttctg ctcccagcgt tcggtaatca
     9901 tatgaaaagg aaagggagag ggaatccgcg acaacaccat agaggttaag ggactgaatc
     9961 cgcgcagtcc ccttaaataa gggcgcaggt tcagtcccgc caatactgat cgcggtatca
    10021 aatgtgccct tcggatatga tttcaggggc ttcttatata cgctcttcac aaaggtctcg
    10081 aggtcagcat tatagactgc taccgaaagt ccatacacgg gtcttttgag atcaaagaat
    10141 tccttgctct caggaaatct cacggtaccc ttgagtactg cggttacggc ctgaccctgc
    10201 tgaggattca gcgtggcagt gagttgtttc acttccagga ggtccctcct gtaatttaca
    10261 tcacctgcta tgtcaccgag ggaatattct tcgtacgctg ctgaatacag cttcatcctc
    10321 ccggcaatga gggggttatc aaaacttcct gttactgttc cagagaattc gccccttccg
    10381 gtcaattcgc ggcggtacgg cagcgtcaca tccgtaagat cgcctgtctt gccttgaatg
    10441 gtaagacgaa gctgggtggt attgatatcc acgtcgccgt aaaaaccaaa gaccgatttc
    10501 tcggtgctcg cctctgaatc agaaagggta aagacattat ttcgggaaac aaaagaaccc
    10561 ttgatctttc tgatcctccc cagcacgtct ttcccctgcc ccgaagcttc ataaacatac
    10621 cagcctgagg gattaaagct cgtccccgaa gtgctcacct cgcccttcac ctttccaaag
    10681 gggatatcgg gtttccaccc gatgagggta agggcagcag ggctgtccac atcagagaat
    10741 ttcgtcttca gcgcataata ttcatcaccc gatacggaca gggtcagttc tgcttccgca
    10801 tgcccgtgat agagatcggc cttcccttcc aggaaacgta atgagcggtc gccatacagc
    10861 actgagcatc tgagttcatc cacatcaata tcaaacagat tgcccttctt tagtcgcgcc
    10921 tttgcggtcc ctgtgagacg gttcaggggt cctttcagcg tgcctgaaaa atccacaaac
    10981 ccttcgacac gttccttcac cttcaatagt tccatgagtg tctgcatgta aaagtcgcct
    11041 ttcaactcca tgtccattac agactgctga agatcgtcca tcgtaagacg cacggttccg
    11101 ctcgcataga tctcaccctc tccctgcctt ttcaggccaa atatcttctt aaacgattca
    11161 gggaggagac ctgcccgaag gtgaaaatcc ccggagttct taggcagact cttactccct
    11221 tcggacgaaa agataccgct cgcattaatc aaggagccat agagcccgac ctggagccct
    11281 ttcacgtcca gttgcccgtc tttcagcact acagttcctt tcacctcggc cttcaattca
    11341 ggccaaccct tgatggtgga agaaagttcc ctcaggctga aacttatctt tggaggggca
    11401 tgccgttgtg cgataaagtt ttcccacgcg ttgagtatgg cctcgccgct cacaccactc
    11461 gcccgaaatg tgttttcttt gtattggaga gtaaatcgtc cgttatccag tgccactgct
    11521 ttgaccttca ccgtgagggg cgtcttcctc tcctcttcga gatacttttt tatgttctct
    11581 atgatagttt ccacctggga ggagctacac gtgatcacag gctcctacac cgtaatcctt
    11641 ctcaatacga gccgcttcct caggagcccc gaggcttcta tatagccttt cacccttggc
    11701 aggtggacaa tttcgatgcc ctcatcaaga accttgatcc cctttgcctc gataaacaga
    11761 gggaacaggt tgagcgtgat tttctgagcc atcacctgtt tcccggtcag tgaagagagc
    11821 tctggaagga tgagattctt aaggagattg gatatgtgag gaccgcgaaa gaacaccatg
    11881 gtgaggacaa cgatcaccac cgggacggca acaaagagaa tcttctttac tgtaatcttt
    11941 cccataatga aagcaggagt tcatggaccg tcatcccctc aagctttttt cctctctgct
    12001 gcagggagag gatgtcgttc gcaatccctg actcagtgtt aatccttgca gatattatat
    12061 caatatcctt ctcatgaatc aggctcgtga catcgtagag gagtccaagc ctgtcctggg
    12121 caaaaaattc aagagtactg ctctctctgg aggtctcatt atcaatttcg ataaagggac
    12181 cgaaccttcc cataacctcg ggcttcacat cggagcaccc cggtaaaacc ctgttttcct
    12241 gaatgcccag tctgcgccac ttctctcctt cgccgctgca taccgcctcc cgaagcctgc
    12301 tctccaggtc ctggcagagc ccgtcccaac agatatctcc ccagttggca atctggattt
    12361 tatcaattac gatccccccg cttcccgtat atacccttgc ccggtagata ttcattccca
    12421 gagaggatag aacaccgacg attcttgaaa aaagacccgg cctgtcccac gcaccgacga
    12481 tgatctctgc ggtgcctgcc ggatcctcct tcaccctgac tccaaaaccc tttcccgtga
    12541 cctcccggta aagcgcgtag tccccggaga ccctttccgg tgttgatgaa ataatatacc
    12601 tctctggcat taacgcgaga aaccgctctg tccctaccct ctcgttatcg gaacggaaca
    12661 tcgttcttga gagtggatga tttccccgca caccactaaa gccctggctc ccgggtaaaa
    12721 caccgacaaa tccgctgaga tactgtgagg tgatctcgta gagctccctg aggagatagg
    12781 ccttccattc agtccagaaa tcaggaccca ccgaggcgat gtctgcatag gtaaggaggt
    12841 agagggcgtc aagattttct ctgtctccca catcatcggc aaactgggct atcacctcaa
    12901 tatcatctgt atctcctttg aaagcgacgg atgacatcaa tgtatggttc ttcacaagaa
    12961 actcgattct cgtcctcagt ccgaccgtga ggttgaattt ctcgagcacg ttctttactt
    13021 ccaggtaacc tgcttcctca tgatggccgc ctccggaacc atagcggtaa ctcttcacaa
    13081 tccccttctt cccgatatcg tggaggagaa gcgagaagat aagcgcttct ttatgcctta
    13141 cctccctgaa cacggccgaa aggtgttcaa gattcttata tttcgtatct ctgagttcaa
    13201 ccagcttctt gaccgcgtag agggcatgtt catctaccgt atagcgatga tagggttcgt
    13261 acaccacaag aaaactcagc gcaccaaatt ccggaatcag tcttcccagc acacggctgt
    13321 tgtgcatctc cctcaatgtt tcatatgtcc tgtctcccct gataatattc ataaaagatt
    13381 caaccgcctt ctgggaactt ctcgtcgtct ttccgatgcg gtggagattt ttcccgattt
    13441 cctccctaag cagcgggctg aaccgctttc cggtctttgc taggacagaa aatgcctcga
    13501 taatgctttc gggcctttcc ttcaggaagt caccattcgc aacgatccgg ttctttgaaa
    13561 gggaaaagtc gtcggttatc cttttcttgg catagaggaa aggtaatatt ctgctgtctc
    13621 tttcttccct gcccgtcctg ggcatgctga acaggtcaag actcctggat gtgatatcat
    13681 tgattgccga tgccttgagg tagaaatacc gcataaaccg ttcggatgag agaaacctct
    13741 tagaagcctt gaaattcagc atttccgcga tgccgtcatg gtattcgaag gagagaatat
    13801 catttctccg cccgctgaga acatggagac agaacctggc cttcagcagg agatcatagg
    13861 catttgcaag ccttttgaat tcttccggag agagtatctt ccctagctca tcgaagctcc
    13921 ttatcctcag tttgatgctg gccagccata caagggtatg gatgtccctc agtccccccc
    13981 tcccttcctt aacatgcggt tcgagcataa aaacggagtc gcttatattc ctgtgccgca
    14041 tctcgatctc gcgcaacttc tcagtgatga acctacccgt ctccctgaat gcaatctcgg
    14101 gatatacggt gtccataaaa tactggtaaa ggtctctgtc acccgctata taacgatgtt
    14161 cgagaagcga agtccgcgtt ttgacatctt tccttgcctc actgatacag tcagggggcg
    14221 tcctgaatga atggccgata gcccgattcg tgtcccaaag cttatagaga aattccgagg
    14281 cctttcctga cgcagacctg tctctggcaa agagcatgat gtctatatcg gaaaagggag
    14341 cgagttcacc tctgccgtat ccgccgaccg ccatgaggca gagcccggaa tgcgcattca
    14401 ttgaggagaa gaggttcttg agaagcgaat cggtcctgcc agtaaaaagg gtcgttaatt
    14461 gcctgccgct gtaaccctgt tttataagtt cagaaagctc atggatatcc actatatcct
    14521 tatctaaatc ccttcttttt ctgcttatta tatcatgagc aggggcgcac tcgtgtgtgc
    14581 gcccggtttt cgtcgcccga ttcgctttag gggcaatccc ctcgtggttg ctcttttaca
    14641 ttggatttcc ccatatcagg gcagacatag gggcatagcc ctaccttctc aattattcca
    14701 tgaaaatgaa tggactcgat aacacattcc tctctctcgt ccgtctccat cctgttttca
    14761 attacctgcc tgttatcgtg acatgaacgt agcgatgcta ataggagtac aagaaaaccg
    14821 agtgttttaa ttagtgcatt aattataggt tggtattcct tcaaaaaagc gtttaaatgt
    14881 ttcagtttac ttcttcgtgt cttcaggggt tttgccttat ctttcttcat atctggttta
    14941 tatcagaaaa gataaaactg ttctggcaga atagtcgttg tctcatgaaa ttacctatac
    15001 cagaaaagct ttgcacagtc tggcagggga aaggaaaaca attttagagt gcggaagtgg
    15061 ctgacagttg catctatctg agagatagat tatggaggtt caacctcaat agatgctcaa
    15121 agctcttatg cttcttgtct ccttcttagc aattttaaga atctcagacc ctcatgacta
    15181 tgattttctt caaaagggtc gcctaccctc caaacctatc atgcggcact gagcttcata
    15241 ttgatcattt agacgacgtt ttgcctcttc tatcaataca tgatatgtcc agaagactac
    15301 tccgtcgaat tttgtgtcaa caaggcgttc tttatgctct aatccacgcc caataattac
    15361 caagcgttct tttggtcctg tcaaaaatct gaactctcac gatgcgttgc gcacagcatc
    15421 ggaatcaatg aacaataccc atttcgagat ttgttgcctc gcgtgattag caggagctgt
    15481 cagatccatc gtttttgtaa agacagcatg aaccggagac tccaactcta taagcagcga
    15541 agaaagccct tgctcgcgtt ccaacacaaa gtcagcttta cagctatccg tcccataata
    15601 gagagtatgc tcaaaaaaac agcgtttgtg cggaggaagc agaacgttct tgtgctcata
    15661 gatgaacctg tgaattctcc tctccaagaa agcctttctc tttataagtg catgaaattt
    15721 ccccagtata gtttttaact ctacaacata tgagctgtgc gtctttggaa ggcgcctctt
    15781 attccagaag tcgatagcat cttgtttcgc attttctaca ttgaatatgc tttgaaaacc
    15841 tttctttctt cccaaaccta ataagcattt gttgtccaac gccggtggca aaagtgggtt
    15901 gtctgctgag tacattacag tgtcgcccca aagagcaaag aaattctcgt cgaggcgttc
    15961 tatcggaggg ccgtcatatc ccagttgagc aaacaagtct cttatttgct caacggactt
    16021 aatttctaag atattcacca catcatcatc aagtgccttg tcaggtacta ctttcaaaac
    16081 aatcccgtca ttacccgcaa caccaatagt tattgtcctc gttgaaagcg cccaactcgg
    16141 aagtccgatc agttctcctt ccttatgcag cgataagata tcaaggcttt catccaggca
    16201 tctgaggtac tcttttattt gtttctctat ttccataatg tcctctcaaa actccagacc
    16261 aataaagaga ttctttcgga gacattcatt aatctctcct cattaaggcg cgcccggagg
    16321 acggtctttt cgtgtctgtt tgtcacaaca taaccaactg tttggcagca ccggccttta
    16381 accattcagg aattttccta agcggcaccc cggcaagctc ttttggttca attttatgca
    16441 gccctccacc gtatgacctt cctgctcgta gcacttcttc aatcgatatt ccatttaaca
    16501 actccagcat ctcaacgaaa cgtgccttat cctcacgtaa aagcgctgat agcagctcat
    16561 tcgggtacag gttcaaataa acattggtca ccagcgcgtt ggacaagttc agtagaaacc
    16621 ggaacggcga tttggtgccg ttttgtgaac gaccgatata tgtggccaaa aaaggactcg
    16681 gcgttctccg ttcctggtaa taccattcct ccctgccggc gcacaggtaa cgctgcggaa
    16741 tattcttttc aactcccctt ttgaagtaat tccacagacc cgaataccgt tcttttacga
    16801 tctcaggcgg cacgtcacaa tcgaggaggt acttaacttt gtcaattctc ggtgacccgt
    16861 ctgcctcgct ttgaataaca gtatccttaa gatatcgcgg actgggcaat attggcttga
    16921 gaaaccccgc aggtatcgaa tagtgatcaa tgatgccctt atcaaggata aagaattcgt
    16981 ttgcccctgt tgccaaccca cgcttgatag tgaaaaaatc tcctatttca tattcttcgt
    17041 catctctttc tgcttcgtcc tttaaatgca agaccgtcca cttgccgttt aaagtaccgt
    17101 tactcagagc aatctcctgt gagtgcttgg ggtttaacat atctgcaccg aaagaaaagc
    17161 ggaatgtcgc gtcaggcgag ggaagacgtt tcatataggt aacgacgcaa gatgacacca
    17221 gggcatcgtc aaattggact tcctccgcat cgaattgatg aatattaagc aaagtgacgt
    17281 gatggcggaa gtaatcacgc agggctttgc cataattgac ataaaggaat tcggaaggaa
    17341 caagccaaga cgcaactgca ttatttgcta gtattgaatg cgccagcaaa ataaaatata
    17401 catataatcc cgacaatcca ctgacagaaa ttccgagttc tctgtcgaga ctcgtttgta
    17461 aactccgttt cgtctcagga ttaatgtggt gatgtctgac ataaggaggg ttagtgcaga
    17521 gaagattata gcggtttgcc tgctcaggac ttcttgaaaa agaaataaaa tcgatctgct
    17581 ctaccgtaag gccgtattga ccccaaattg cccgtgatat ttctgcatag gtcgggtcta
    17641 attctacccc gactgcctcc ttaagcgtgc gactgtttgc aatacgtctt aaagcagaaa
    17701 aaaagacacc agttcccaag cccggctcta taaaggaaac tggctcatct ttgggaagat
    17761 aagctaaagc atttgccaat atttcgagag c
//
LOCUS       DATKOU010000009         3368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4631370, whole genome shotgun sequence.
ACCESSION   DATKOU010000009 DATKOU010000000
VERSION     DATKOU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631370"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..647
                     /gene="rpsL"
                     /locus_tag="VMU21_00315"
     CDS             276..647
                     /gene="rpsL"
                     /locus_tag="VMU21_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HUO75994.1"
                     /translation="MPTIAQLVKQGRKKLEVKTKSPALRNCPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNAMEVTSYIPGVGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DSVGVADRRRGRSKYGAKRPK"
     gene            660..1130
                     /gene="rpsG"
                     /locus_tag="VMU21_00320"
     CDS             660..1130
                     /gene="rpsG"
                     /locus_tag="VMU21_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HUO75995.1"
                     /translation="MPRRRVAEKREILPDPKYNSKLVSKFINIMMEDGKKSTSERTCY
                     GAFEIIKEKSGNDPLKVFKTAIENVKPVLEVKPRRVGGATYQVPVEIRQTRRMALAFR
                     WIITYSRGRNEKTMRERLAGELMDAFNNTGTSIKKKEDTHRMAEANKAFAHYRW"
     gene            1149..3227
                     /gene="fusA"
                     /locus_tag="VMU21_00325"
     CDS             1149..3227
                     /gene="fusA"
                     /locus_tag="VMU21_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUO75996.1"
                     /translation="MSRLPLEKTRNIGIMAHIDAGKTTTTERILYYTGVTYKIGEVHE
                     GTAVMDWMVQEQERGITITSAATTCFWKDHRINIIDTPGHVDFTIEVERSLRVLDGAV
                     AAFDSVAGVEPQSETVWRQANKYGVPRIAFMNKMDRVGADFFMSVKSMVDRLGANPVP
                     IQIPIGAEDKFRGSIDLVRMKAIYFDDETLGAKYVEDTIPDGLVPQAREYREKMLEAL
                     SDVDEGIMEKFLGGEKINEEEIMRALRKGTIELRLTPVICGSAFKNKGVQLLLDSIVD
                     YLPSPLDVPPVIGINPSDDSEVVRKANNDEPFSALAFKVMTDPFVGQLTFIRVYSGVL
                     SAGSYIYNSTKDSKERVGRLLRMHANKREETKEVAAGDIVAAVGLKNTLTGDTLCDEK
                     HPVILESIEFPEPVISVAIEPKTKADQEKLSQSLAKLAQEDPSFRVSFNEETGQTIIA
                     GMGELHLEIIVDRLLREFKVGANVGKPQVAYKETIRMASKAEGKFVRQSGGRGQYGHV
                     YIELEPAEAGKGFEFVNKIVGGTIPREYVPAVEKGVKEAMDTGVLAGYPVVDVKAVLY
                     DGSYHEVDSSEMAFKIAGSMAFKEAAKKAKPVILEPIMSVEVVTPEEYMGDVMGDLNS
                     RRGKIQSMEKRGNAQVIRAEVPLSEMFGYATDLRSKTQGRATYTMQFAHYEDVPKGIA
                     DTIVAKVKGE"
     gene            3238..>3368
                     /locus_tag="VMU21_00330"
     CDS             3238..>3368
                     /locus_tag="VMU21_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUO75997.1"
                     /translation="MAKAKFERVKPHVNVGTIGHVDHGKTTLTAAITKVLAIKGQAT"
BASE COUNT          964 a          704 c          978 g          722 t
ORIGIN      
        1 gaaggttatc tttgtcgtgc gggagggaag atgaatgaaa gaattctttt gagtgaacta
       61 tttttggcgc ttaccccatt gcttttttcc tcttcgaaaa gttattctat caacccccac
      121 tggtggagtc aacacgatta ccgggagggg attacgggaa cagctttaag tacttgacaa
      181 aaaaagtttg ttctggtaag ctttcaaagt tttcccgtaa aaacctcgag acctatagtt
      241 tcgaaagttt ctcgtgtgaa aaatatggag gaaaagtgcc gacaatagct caattggtga
      301 aacaagggcg gaagaagctg gaagtgaaga ccaagagccc tgccctcagg aactgccctc
      361 agaagagggg agtatgcgtg agggtgtata ccaccacccc caagaaacca aactctgcat
      421 tgaggaaggt cgcgagggtg aggctgacca atgccatgga ggtaacttcc tatatccccg
      481 gagtgggaca caatctccag gagcactcca ttgtgatgat aagggggggg agggtaaagg
      541 accttcccgg cgtaaggtac cacatcatca gaggcaccct tgactccgtg ggcgttgcgg
      601 acaggagacg ggggagatca aaatacggag ccaagaggcc caagtaagag agaacggata
      661 tgccaagaag aagagttgca gaaaagagag aaatccttcc agacccaaaa tacaacagca
      721 agctggtgag taagttcatt aatataatga tggaggacgg caaaaagtcc acatcagaga
      781 gaacctgtta tggtgcattc gagatcatta aggagaagtc agggaatgac cccctgaagg
      841 tctttaagac tgccatagag aatgtcaaac ccgttttgga ggtaaagccg agaagggtgg
      901 gcggtgcaac gtaccaggtt ccggtcgaga tacgccagac gaggcgcatg gccctggcct
      961 tcagatggat tatcacatat tcccgcggaa ggaacgagaa gaccatgagg gagaggctcg
     1021 caggtgagtt gatggatgcg tttaacaata cagggacttc cataaagaag aaggaagaca
     1081 cccaccggat ggctgaagcc aataaggcgt ttgcccatta tagatggtaa ggatagagga
     1141 atacgcactt gtcaagatta cctttagaaa agacacgcaa tattggcatt atggctcaca
     1201 tcgatgccgg gaagaccacc actaccgagc gtatccttta ctacacaggg gttacctata
     1261 aaataggcga ggtccatgaa ggcactgcgg tgatggattg gatggtccag gagcaggaga
     1321 gaggaatcac catcacctca gctgcaacca cgtgtttctg gaaagaccac aggattaata
     1381 ttattgacac cccgggacac gttgatttta ccatagaggt cgaaaggtcc ctcagggttc
     1441 tggatggcgc ggtggctgca tttgattctg ttgcaggggt tgagccccaa tccgagactg
     1501 tctggagaca ggccaacaaa tatggagtgc cgagaatcgc gttcatgaac aaaatggaca
     1561 gggtgggcgc cgactttttc atgagcgtga agagcatggt tgaccgtctt ggcgcgaatc
     1621 ctgtgcccat acagatcccc ataggtgcgg aagacaagtt taggggctcc atagacctgg
     1681 tgagaatgaa ggccatttat tttgacgatg agacccttgg ggcgaaatat gtggaggata
     1741 ccatccctga cgggttagtc ccccaggcaa gggaatacag agaaaagatg ctggaagcac
     1801 tctctgatgt ggatgaagga attatggaga aatttctggg tggcgagaag ataaatgagg
     1861 aagagatcat gagggccctg agaaaaggaa ccatcgaatt gagacttacc cctgtcatat
     1921 gcggttctgc ctttaaaaac aagggagttc agctcctcct tgactccatt gtggattacc
     1981 ttccctcccc cctcgatgtt cctcctgtga taggcattaa tccttctgat gactcagagg
     2041 tggtgagaaa ggcgaacaac gatgaaccct tttccgccct tgcatttaag gtaatgaccg
     2101 atccctttgt gggacagttg acgttcataa gggtatattc cggtgtgttg agtgccggtt
     2161 cttatatcta taactccaca aaagattcca aggagagggt gggtaggctt ttgaggatgc
     2221 acgccaataa gcgtgaggag acaaaagagg ttgctgccgg tgacattgta gcagcagtgg
     2281 gactgaaaaa cacccttacc ggggataccc tctgtgacga aaaacaccct gtgattctgg
     2341 aatccatcga atttcctgag cctgtgatct ctgttgccat agagccaaag acaaaggctg
     2401 atcaggagaa gctctctcaa tcccttgcca aacttgccca ggaggaccct tcattcaggg
     2461 tgtcttttaa tgaagagaca ggccagacca ttattgcggg tatgggcgaa ctccatcttg
     2521 agataattgt ggacaggctg ctcagggaat ttaaggtcgg cgctaatgtg gggaaacccc
     2581 aggttgctta caaggagacc atccgcatgg catccaaggc agaagggaaa tttgtgagac
     2641 agagcggagg gcgcggtcag tacggacatg tttatattga gctcgaaccc gcggaggcag
     2701 ggaaggggtt cgaattcgtg aataaaattg tgggggggac tatccccagg gaatatgttc
     2761 ctgcagtgga aaagggagtg aaagaggcga tggatacagg agtccttgca ggataccctg
     2821 tggtggatgt gaaggcagtc ctctatgacg gctcctacca tgaggttgac tcatcagaaa
     2881 tggcattcaa gattgccggt tccatggcct ttaaggaggc tgcgaagaag gcaaagcccg
     2941 ttatccttga gccgataatg agcgtggagg tggtgacccc tgaggaatac atgggcgacg
     3001 taatgggcga cctgaattca cggaggggaa agatacagtc catggaaaag agagggaatg
     3061 cccaggtgat cagggcagag gtccctcttt cagaaatgtt cggctatgcc actgacctta
     3121 gatcaaagac ccagggaagg gcgacctata ccatgcagtt tgcccattat gaggacgtgc
     3181 caaagggcat agccgatacc attgtggcca aggtgaaagg ggaataaagg agggaaaatg
     3241 gcaaaggcga aatttgagag ggtaaagcct cacgtaaacg tagggacgat tgggcatgta
     3301 gaccatggga agacgacatt gacggcggcg atcacgaagg tattggcgat caaggggcag
     3361 gcgacgta
//
LOCUS       DATKOU010000010         7139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1553047, whole genome shotgun sequence.
ACCESSION   DATKOU010000010 DATKOU010000000
VERSION     DATKOU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553047"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="VMU21_00335"
     CDS             <1..1569
                     /locus_tag="VMU21_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HUO75998.1"
                     /translation="SYFLVTLDRGEESSKAGLVYAVMTHIGTAFIIAAFLVLYRYTGH
                     MDFAGIKGASMQLAPGIRSAVFLLFLAGFGTKAGIIPLHTWLPRAHPAAPSNISSLMS
                     GVMIKTGIYGMVRMNMDLLGQGAEWWGIALIVIGAISSILGVLYALMEHDIKRLLAYH
                     SVENIGIILLGMGASMMFMSNGLYSLATIALTAGLYHTLNHAVFKGLLFLGAGSVVHG
                     THTKNMEDMGGLIKTMPYTGFFFLVGSVSICALPPFNGFVSEWLTFQSLLLGFESPSV
                     ISKIVSPLGGAALALTGALAASCFVKAFGISFLGIPRSHHAENVKESSPSMLIGMAFL
                     ALFCLLFGLFPGMVIAVISPAVFSLTGVPGMSPGITIVSISETASSLSPAGILIAIAV
                     MAVATVAFLLSVGGRGKTLYSDSWDCGMPSLTPRMQYTATAFTKPIRMIFKRIYLPKR
                     ELSVSYFLKPFFVKSITYSGEITPFFERYLYEPGTRVIHAIAGKVRLLQSGSLHLYLG
                     YILVTLIVLLIFGS"
     gene            1571..2488
                     /locus_tag="VMU21_00340"
     CDS             1571..2488
                     /locus_tag="VMU21_00340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HUO75999.1"
                     /translation="MEYLQFLIILLFSPLIQGMIKKMKARMQGRVGSGIFQPYYDLIR
                     LMKKEMVISHVTSWIFKATPYIVLASTIVASMITPVLTTRVPFGIMGDVIALIYILAT
                     GRFFMALAGLDAGTAFGGEGSSREMAVAILVEPMMMLSIFTAAISVGSTNIARIAEAQ
                     NMVYSPSHLLALSAFVVAIIAETGRIPVDNPDTHLELTMIHEGMLLEYSGRYLALMEW
                     AHYMKQMLLFTLGVDIFFPVGIPSDTAFAGLGVSFALYFLKMGFMAVLMAAIESTRAK
                     LRFFQLPSLLGGAFVLAFLSLLTYIMMGR"
     gene            2496..3155
                     /locus_tag="VMU21_00345"
     CDS             2496..3155
                     /locus_tag="VMU21_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76000.1"
                     /translation="MKNWIQAFSVMDFISVGILLTAVAMNAFKRLESCVKIYTLNSLL
                     LSSLIAIVALMVGEPHLYIASAATLLGKGILIPMFLKRVVRQMKVTHDVEPYINNALS
                     LAISGILVAVVYASLRQGIFVSGFSKNVLQISIAVILIGLFVMITRRKAITQVIGLLF
                     MENGLFLAGFSLTYGMPIIIELGVLFDMLMGVIILGIFAVQIKRAFASSNLDNLTILK
                     G"
     gene            3161..4678
                     /locus_tag="VMU21_00350"
     CDS             3161..4678
                     /locus_tag="VMU21_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HUO76001.1"
                     /translation="MIAILLIPLVSSALCYLLKSPRGIGYASMSGAVVLAAVSLPTIL
                     SVVSSPAEIMNGMLYMDALSGYIMAMVVFLGLASALYSISYLEHELNARLTDITGIRR
                     YYALLHLFIFTMLLVTVANNLALMWIAIEATTIVSALLIGLGFYKRHLAIEAAWKYII
                     LCTVGITFALLGTFITYYASTAVMGTEGALNWTVLKGIAGSLNPSTMKLAFIFILVGY
                     GTKAGLAPMHNWLPDAHSQAPSPISALLSGILLNTAFYGVMRFVSIVEPSTGDLFTGH
                     LLILFGLISIGLSSVLILVQDNYKRLLAYSSIEHMGIISLGVGIGGPLGIYGALLHIL
                     NHALSKPLMFFASGKIQSHYGSTRIENIRGVVSSMPLLGTLTFVGAFSLAGTPPFNIF
                     ISEFTILRAAVDKALWAVVVLFLIFVVLVFYGMLSGFGKMLFGKSEDREETRAKGTES
                     ETVSEFSCFRSCGLSDVLGNAVMIVMAVCIVVLGVTVPDFLDNAIKSCVIILGIR"
     gene            4678..6246
                     /locus_tag="VMU21_00355"
     CDS             4678..6246
                     /locus_tag="VMU21_00355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HUO76002.1"
                     /translation="MERILKKALRTRDHLNERYLHVSRDDFKGTCVHLYAKERAILRL
                     MFGTDEREAEGVFNIYAVFSIPGIDRFDIVVLSLKEDDLTFPSITPEIPAAHWYEREI
                     RDMFGLTPKGHPDLRRLVFHDSFPKDSHPLRKDWSMSEADLKEWGEGIAQKEPYHFMR
                     VEGEGVYEIPVGPVHAGIIEPGHFRFSAVGETIFFLEPRLFYTHKGTEKLFETLGFAK
                     GVGLAERVSGTSSFSHSVAYCMAVERMVGMDIPEKAKAVRTLVLELERLYNHIGDIGN
                     MCAGTGLAVGYAKGAIIKERLMQLNERVSGNRYLRGINIIGGVMGDLFTHADDILMTL
                     DLVTKEYKELMTFLIGTVSHVERLENTGKLSKEIAVKLGVTGVAARASGVNDDIRKAH
                     PHLLYDRLEFEAHTMARGDVFGRMMIRAEEAECSLSMIHALLEKNYRGALTAEMRDIS
                     PYASALGYAETPRGSAFYWVMSGREGNPLRVKLRSPSFCNWPAVPFAVHGNIVPDFPL
                     CNKSFNLSYSGCDM"
     gene            6265..6792
                     /gene="nuoB"
                     /locus_tag="VMU21_00360"
     CDS             6265..6792
                     /gene="nuoB"
                     /locus_tag="VMU21_00360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HUO76003.1"
                     /translation="MIKEILRNIVGRKEALSFPEGFMVSPEHVALNVDLKRKIDGIFG
                     RSLRIRQVDAGSCNACEWECTALTNPVYDIQRFGIDFVASPRHADALLVSGPVSRQME
                     LALKKTYRATPEPRLVIACGDCAVNGGIYRGSYAVSDGVANVVPVDCHIPGCPPTPAG
                     ILLGLIQLIESVSKR"
BASE COUNT         1574 a         1791 c         1910 g         1864 t
ORIGIN      
        1 tcttatttcc tcgttaccct tgacaggggt gaagagtcgt caaaggcagg gctggtgtac
       61 gcggtgatga cccatatcgg cacggccttc attatcgctg cctttctcgt cctttaccgg
      121 tatacagggc atatggactt cgcggggata aagggcgctt caatgcagct ggccccgggg
      181 atacgctcgg ctgtctttct cctgttcctg gcgggattcg ggaccaaggc gggcatcatc
      241 cccctccata catggcttcc cagggcgcat cctgcggccc cgtccaatat ctcatccctc
      301 atgtccggcg taatgatcaa gacaggaatt tacggcatgg tgaggatgaa catggatctc
      361 cttggacagg gggcggaatg gtggggaatc gcccttattg taatcggcgc gatctcctcc
      421 atccttggtg ttctctatgc cctgatggaa catgacataa agaggctcct cgcgtaccac
      481 agtgtggaaa atatcggcat tatcctgctg gggatgggcg catcaatgat gttcatgagc
      541 aatggtctgt atagcctggc taccatcgcc ctcaccgcag gactctacca tacattgaat
      601 catgcggtat tcaagggact gctcttcctc ggggccggct cggtggtgca cggaacgcac
      661 acgaagaaca tggaggacat gggcggcctt atcaagacaa tgccctacac aggctttttc
      721 ttcctcgtgg gttctgtatc catctgtgcg cttcctccct ttaacggttt tgtgagcgaa
      781 tggctcacct tccagtccct gcttcttgga tttgagtccc cctcggtgat ctcaaagatc
      841 gtgtcacctt tgggcggtgc tgcccttgca cttaccggag cccttgcggc gtcatgtttt
      901 gtaaaggcat ttggcatatc ttttctgggc attcccagaa gccatcatgc cgagaatgtg
      961 aaggagtcat cgccctccat gcttatcggg atggccttcc tcgcactttt ctgtctcctc
     1021 ttcggactgt tccctggcat ggtgatcgcg gtcatctctc ctgcggtatt cagcctcacc
     1081 ggcgtccccg gaatgtcgcc gggcattacc attgtctcga tcagtgagac ggcatcttcc
     1141 ctgtctccgg ctggcatact catcgcaatc gccgttatgg ctgtggcaac agtggccttc
     1201 cttctcagtg ttggggggag gggcaaaacg ctttatagtg attcctggga ctgcggtatg
     1261 ccttccctta ccccgaggat gcagtacacg gcaacggcgt tcacaaagcc gatccgcatg
     1321 atctttaagc gaatttatct tcccaaacgc gaactgagtg tttcctattt cctgaagcca
     1381 tttttcgtaa aatccataac atacagcggt gagataaccc ccttctttga gcggtatctc
     1441 tacgaaccgg ggacacgtgt tattcacgca attgcaggga aggtgagact tctccagtca
     1501 gggagcctcc acctctacct tgggtacata ctggtgacgc tcattgttct tctcatattc
     1561 gggagctgat atggaatacc tccagtttct tatcatcctt ctcttctccc ccctgattca
     1621 gggcatgata aagaagatga aggcgcgcat gcaggggagg gttgggtccg ggatattcca
     1681 gccgtattat gacctgatac ggctgatgaa aaaggagatg gtgatatccc atgtgacctc
     1741 ctggatattt aaggcaacac cttacattgt acttgcatcg accattgttg cctcaatgat
     1801 caccccggtg cttaccacaa gggttccctt cggcatcatg ggcgatgtca ttgcgctgat
     1861 ctatattctt gccacaggaa ggtttttcat ggcccttgca gggcttgacg cggggaccgc
     1921 ctttggcggt gagggcagca gcagggagat ggcagtggcg atccttgtgg aaccaatgat
     1981 gatgctgagc atatttaccg cagccatatc ggtaggctcc acaaatattg cgaggattgc
     2041 agaggcacag aatatggtgt attcgccgtc ccaccttctt gccctttccg cctttgttgt
     2101 tgccatcatc gcagagaccg ggaggatacc cgttgacaac cctgacacgc acctggaact
     2161 taccatgatc cacgagggga tgctcctgga atattccggg aggtatcttg cgctcatgga
     2221 gtgggcgcat tatatgaaac agatgctcct tttcaccctt ggggtggaca tctttttccc
     2281 tgtgggaatt cccagtgata ccgcgtttgc agggcttggc gtatcgttcg ctctctattt
     2341 tcttaaaatg ggtttcatgg cggtgctcat ggctgcgata gaatcaacaa gggcaaagct
     2401 gaggttcttt cagcttcctt cccttttggg aggggctttt gtccttgcgt tcctgtcgct
     2461 gctcacctat atcatgatgg ggagatgaat tcattatgaa gaactggatc caggcttttt
     2521 cggtgatgga tttcatatcg gtggggatac tccttaccgc ggttgccatg aacgctttta
     2581 agcggctgga gtcgtgtgtg aagatctaca cgctgaactc attgctgctc tcatctctca
     2641 tcgcgatcgt ggcgcttatg gtcggggaac cccacctcta catcgcatcg gccgctaccc
     2701 tcctgggcaa agggatcctc atccccatgt tcctgaagag agtggtccgg caaatgaagg
     2761 tgacccacga cgtggagccg tatataaaca atgccctttc actcgccata tcagggatcc
     2821 tcgtggcagt tgtgtatgca tcgctgaggc aaggcatatt cgtatcaggg ttttcgaaaa
     2881 acgtactcca gatttccatt gcggtgatcc tcatcggact gttcgtcatg attacccgga
     2941 ggaaagccat tacccaggtg atagggctgc tcttcatgga gaacgggctt ttcctcgccg
     3001 gtttttccct tacctacggg atgcccatca tcatagaact tggcgtgctg ttcgatatgc
     3061 tcatgggagt gatcatactg ggaatatttg cagtccagat caagagggcg tttgcgtctt
     3121 ccaacctcga taatctgacc atactgaagg gataggattg atgatcgcga tactcctgat
     3181 acccctcgtc agctcggcat tgtgttatct gctgaaaagc ccgaggggca taggatatgc
     3241 gagcatgtcc ggagccgttg tccttgcagc agtttctctg cccacgatcc tctctgtggt
     3301 ctcttcccct gcggagataa tgaatgggat gctctatatg gatgctcttt ccggatatat
     3361 catggcgatg gtggtgttcc ttggtcttgc gtcggccctt tactccatca gttatctcga
     3421 acacgaactg aatgctcgcc tcaccgacat aacgggaata aggagatatt acgccctgct
     3481 ccatctcttc atttttacca tgctcctggt gactgttgcg aataatctgg ccctgatgtg
     3541 gatcgccata gaggcgacca ctattgtctc cgcccttctc atagggctcg gtttttacaa
     3601 gaggcatctg gccatagagg ctgcatggaa atatatcatc ctctgcactg tggggattac
     3661 ctttgccctt ctggggacgt tcatcaccta ctatgcctcc actgcggtca tgggaaccga
     3721 aggggcgttg aactggacgg tacttaaggg gattgccggt agcctgaacc cctctaccat
     3781 gaaactcgcg tttatcttta tcctcgtggg atacggtacc aaggcgggat tagcccctat
     3841 gcataactgg cttcctgatg cccacagcca ggcgcccagt cccatcagtg cacttctttc
     3901 cggaatcctg ctgaataccg cattttacgg ggtgatgagg tttgtctcta ttgtggagcc
     3961 ctccacaggg gatcttttta ccggacacct tctcatcctt ttcggtctga tctccatagg
     4021 cctgtcctcg gtattgattc ttgtccagga taactacaag aggctcctcg cctacagcag
     4081 catcgaacac atggggatta tttcccttgg ggtcggcata ggtggccctt tgggcatcta
     4141 cggcgcactt ctgcacatcc tgaaccatgc cctttcgaaa cccctcatgt tctttgcctc
     4201 agggaagatt cagtcacatt atggttcaac acgcattgag aatatacggg gagtcgtgtc
     4261 ttccatgccc ctcctgggga cactcacctt tgtcggcgca ttctccctcg caggcacccc
     4321 gccgttcaat atcttcataa gtgagtttac tatcctgagg gctgcggtgg acaaagccct
     4381 ctgggctgta gtagtgctgt ttctcatctt tgttgtcctc gtcttttacg gaatgctttc
     4441 cgggttcgga aagatgctat tcggaaagtc tgaagacaga gaagaaacac gtgcgaaagg
     4501 aacggagagt gaaacagtat cagaattttc ctgttttcgt tcgtgcggat tgtcggatgt
     4561 cctcgggaat gcggtaatga ttgtaatggc cgtgtgtatc gtagtcctcg gagtcacggt
     4621 tccggatttc cttgacaatg ccataaaatc ctgcgtaata attttaggaa taagatagtg
     4681 gaaagaatac ttaagaaagc gctgagaaca agggatcacc tgaatgaacg ttatctccat
     4741 gtgagcaggg atgattttaa aggcacatgc gtgcatcttt acgcaaagga gcgcgcaatc
     4801 ctccgcctca tgtttggcac ggatgagaga gaggctgagg gcgtcttcaa tatttatgcg
     4861 gtgttttcga ttcctggtat tgaccgcttc gacatcgtgg ttctctccct gaaggaagac
     4921 gacctgacct ttccttccat tactccggaa atccctgcag cccactggta tgagcgggaa
     4981 atacgggaca tgttcggcct tacccctaag ggacatcctg accttcggag gcttgttttc
     5041 cacgattcct ttccaaagga ttcccatcct ctgaggaagg actggagcat gagcgaagct
     5101 gatcttaaag agtggggaga ggggattgct cagaaagagc cctaccattt tatgagggtc
     5161 gaaggggaag gggtttacga gatcccggtg ggacctgtcc acgcggggat catcgagcca
     5221 ggccatttca ggttcagcgc agtgggggaa accatattct tcctggagcc ccggcttttc
     5281 tatactcaca agggcacaga gaagcttttt gagacccttg ggtttgccaa aggggtaggg
     5341 ctcgcggaaa gggtttcagg gacgtcttct ttctcccatt ccgttgcgta ctgcatggct
     5401 gtggaacgga tggtaggtat ggatattccg gagaaggcaa aggccgtaag aaccctggtg
     5461 ctggaactgg agcgacttta taaccatata ggggacatcg ggaacatgtg cgcaggaacc
     5521 gggcttgccg tgggatacgc aaagggcgcg atcatcaagg agcggcttat gcagctcaac
     5581 gagagggttt cggggaacag atacctgaga ggaataaata tcattggcgg agttatgggg
     5641 gacctgttca ctcatgccga tgatatcttg atgacgttgg accttgtcac aaaggaatac
     5701 aaggagctta tgacattcct catcgggacg gtatctcatg ttgaacgcct tgagaacacg
     5761 ggaaagctct caaaggagat cgcagtaaaa ctcggggtca ccggtgtggc agcaagggct
     5821 tcaggggtga atgatgacat aagaaaggca cacccccacc tcctttatga cagacttgaa
     5881 tttgaagccc acaccatggc acggggcgat gtatttggga gaatgatgat cagggccgaa
     5941 gaggccgagt gttcgctatc catgattcac gcactcctcg aaaaaaatta taggggcgca
     6001 ctgacagctg agatgaggga catttcccct tatgcgtctg ccctcggcta cgcggagaca
     6061 ccaaggggtt cggcgtttta ctgggtgatg tcaggaaggg agggaaaccc tctcagggtg
     6121 aaactgaggt ccccctcttt ctgcaactgg cccgcggttc cctttgcggt acacggcaat
     6181 atagtgcctg atttcccgct ctgcaacaag agttttaatc tttcgtactc aggatgtgac
     6241 atgtgaggga gatggcgaac gctgatgatt aaagagattc tgagaaatat agtgggacga
     6301 aaggaggccc tttcattccc tgaaggtttt atggtttctc ctgagcatgt cgctcttaac
     6361 gtggacctca agagaaagat cgatggcatt tttggaaggt ctctgaggat acggcaggtg
     6421 gacgcgggtt catgcaatgc ctgtgagtgg gaatgcacag ccctcacgaa tcctgtctat
     6481 gacattcaga ggtttggtat tgatttcgtc gcgtcaccga gacatgcaga tgccctcctg
     6541 gtgagcggtc ctgtttcgag gcagatggaa ctcgccctga aaaagacata ccgtgcaaca
     6601 cccgaaccaa gacttgtcat cgcatgcggt gactgtgcag tgaacggagg tatttatagg
     6661 gggagctatg cggtgagcga cggtgtggcg aatgtggtgc ctgttgactg tcacatcccg
     6721 ggatgccccc cgacaccggc cgggatactt ttgggactca tacaacttat tgagtctgtt
     6781 tcaaaacgtt aatcccatcc tattaagagt ctcgaggata agcaccatcc aattctgtcc
     6841 cggacggtct tccccctgtg aaagcatacg ggagcttgag cggccatttg aggagatagt
     6901 caaaaaaagg caggattatc aagcgcgagg tggcagggca gggtggaggc gaggctttgc
     6961 caggatgagg gagagagatg acctcaggcg aaagatcaac acgtttcaag taatgacttt
     7021 tgcagaccac atggtttatc ataccagcac tacatcctat ggaagaagtc tacagtataa
     7081 agcttcctgt ttttgaaggg cccctggacc ttcttctcca tcttatcaag gaaaacaag
//
LOCUS       DATKOU010000011        11061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_400917, whole genome shotgun sequence.
ACCESSION   DATKOU010000011 DATKOU010000000
VERSION     DATKOU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400917"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VMU21_00365"
     CDS             <1..510
                     /locus_tag="VMU21_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76004.1"
                     /translation="KGYHVLLDAIPLVLKKAPHAIFAFVGDGPQKENISGTIEQLGLS
                     GNVRLLGMRWDIPNVLKSIDIFALPTLQEALGSAFVEAMAMGKPVIGTNVGGVSEVIK
                     DGSNGYLVEPNNAAALSEAILKVLEDPERGRSMGREGRRMVEELFTNEKMCEKMHDLY
                     MSLLQGKRS"
     gene            570..1292
                     /locus_tag="VMU21_00370"
     CDS             570..1292
                     /locus_tag="VMU21_00370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUO76005.1"
                     /translation="MTPTTFERQMEYLSEAGYRTLTLDELLSYIKGDLVLQQKAVVVT
                     FDDGWLDNYIYAFPVLKKYNIHASIFIVTNIIGRASRDGGKPPTVVPTHRESKLIMQK
                     GEEHEVFLRWDIIKEMAESELIDFFSHAKTHASCDFLSEPELSEELEASKHVIEERLK
                     KPCPFLCWPFGRYTSFAIEVARKVGYSATYTTNHGIVTPGSDPFAIKRIGVKDDMGWF
                     KSRMRIYSNLLLARIYLKLKRK"
     gene            1289..2407
                     /locus_tag="VMU21_00375"
     CDS             1289..2407
                     /locus_tag="VMU21_00375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO76006.1"
                     /translation="MRISPADGLLFLVMRLLKSLDKRKRDVKYFDPSSVNRILVVSST
                     AIGDTLLSTPAIKTVRERYPHADIVAHFNIKNMELFDNNPHINGIIPYYGGYKKFFGT
                     VSKFRKHKFDLALIFHGNEPQASPTAYLSGIRFIMKLPNNGEYRFLLSNTEPVMSWLD
                     FGHAIEQRLKVAELAGCVASGLPRMELPLRGEGENAVDAFLDGSNITPSDRLVGFQVG
                     ASTLSRMWFADRFIELGRRLIHELGDIQIVITGSPQEYGYCKKIADAIGTKAFVSAGK
                     IPLRYVPSLVRRLRVLVTGDTGIMHMAIATGTPIVALFAVSDSQRSGPYYDKEKHRVI
                     QKEKTCDPCTSKKCTYQRCMENISVEEVFCELMEVIER"
     gene            2404..3318
                     /locus_tag="VMU21_00380"
     CDS             2404..3318
                     /locus_tag="VMU21_00380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO76007.1"
                     /translation="MSNQLCPVCKGITAPFFVGKRDLLECIHCHIVFDTRVSYDRNFY
                     EHQRAASVDATKIDARRRNVRQRINLIKTLLHKDISLLDIGCGEGVFLREVLADVREA
                     TGLEPTLFYVNYAREHFNLDVRQGLIEDVDFPDGAFSVITMFHVIEHVDDPVAALKKI
                     GRWLKTGGHLVIEVPNIKSPTARYKGLDWEMIIPEHRFQFYGESLRYVLDRAGFRLIS
                     LRTRDFDQYRTSIGKNLRKLGIGTQEYRPKAPSVGHEVRVPGIPGGPISPAKRIRRFI
                     QAPLKALLGWLVMKLARGDYLFVVARKA"
     gene            3448..4668
                     /locus_tag="VMU21_00385"
     CDS             3448..4668
                     /locus_tag="VMU21_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76008.1"
                     /translation="MTIIPKHILILDTGKEWGGGTNSLLELLGRIDRKAFRFTGVFYH
                     DYQKGKESTIKTELEKMGIPFMLLKQEKQPVMAKFLKELLRCMFFFSKKLRRYLVFSV
                     DYHVRIKPNAEKIVEILKNLKAALLYLNNQPSSNLEGILAAQQVGVPIIQHARSNASL
                     LPAEVKMINRNITSMICVSNGLKENYIKQGVLGNIVTVVHNGIDVTREPEVPREIVLR
                     SLDAGKDEVVVGMVGSLMKRKGFDIFIRALRHLKDAGAQFKGVIIGDGPEKEHLSRLV
                     EKFNLTERIVFTGFRADGLSYINALDILVLSSPEEGFPRVILEAMLMNKPVVVSDIPG
                     PSELVVHNETGYLVPAGQPSAFADCLRRLIAQPEMRKQMGKKGRERVIEHFSVEKYVH
                     GVDSVFKKVLGLKK"
     gene            4665..5321
                     /locus_tag="VMU21_00390"
     CDS             4665..5321
                     /locus_tag="VMU21_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUO76009.1"
                     /translation="MTAEMSNTHESIPWSDIKARREEVKNSYPTLFTIPLMYCTKREL
                     IGNVVKTIEGKVLDIGAADRFVRDICAQVGKEIEYRSMDVDRTGFHEYYSLDAVQETF
                     DAIFLLDMIEHISLREGILLLQRCKEMLNPDGRIILTLPNNNHPTAFASDCTHVTSYR
                     YHEVGGTLLAAGFEELQIFRISAKRRLKHRLLATLLTPVLKFLDMDFAPGIFLTAKRK
                     "
     gene            5325..6449
                     /locus_tag="VMU21_00395"
     CDS             5325..6449
                     /locus_tag="VMU21_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO76010.1"
                     /translation="MLYIVITYLAAPVLYVLILLRRRKQIKNILVIQTAKIGDVICST
                     PVFREIKKGYPEAHLTVMATPVTLELLEINPHVDEIITMNHKDYKGPSGKLRLANLLR
                     KRKFDIAVCLNPNVLFACATFWGLVPVRLSVMPDFSGSTFKLAAFFFSSLERHVKGRL
                     VIETYLRMLRAIGIESNNISKEVYKSETAENRVSQRIGELKKPLIGIAVSSGNKLKEM
                     GPEKIIQLAAILLDTTDVFIVLIGSSQDRGTSSLIRKALGKKERILDVTGEFGLGELP
                     ALIERLSLLVGVDTGIVYMADALSVPVIDIAGPSDMEDQRPTGAKSMILQKKVQCVPC
                     SHAFHAPYACVRNTRECIQLMSIDEICYAIKKFFPEVHCP"
     gene            6449..7759
                     /locus_tag="VMU21_00400"
     CDS             6449..7759
                     /locus_tag="VMU21_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUO76011.1"
                     /translation="MNILNNLTLSHGVRFFLYAFIFLQPVNRFNSSREIAFGGLLVFF
                     VLKLFKGGLKDIRLSDGTIVALFLLTAWSLMVSLLGPYPVDSLSAMKNNLLAQILIFF
                     AIITEFKTLRELNGLFWIVISSFLLVTLISVFEVVNLTNYDQLLKSHDMFTHKMFIGG
                     YANHATFYLPFILAWLIAFDEPRVRRWVGLGTLITGIVVVFVYSHRTALIALPSAFFI
                     ILLLTRKYKILLAACLFVIIAAAAIGLSKSDVLSRYKSLGNPKTYITNRGVSGRFAVW
                     EGALQIIKERPILGYGYGWKKIAWVAQDLNLADSWKKDFPHIYAYYVGEAHLGYGRVN
                     PHSLALQILFEIGVVGLLIFLWFWATIIHKIIKVGFSKEHSDSRSYVLCSVGVLASYL
                     IINIPNGFWEDSYGRVIFFFIASIFVAFRQHVNGLTPQESFTCK"
     gene            7750..8772
                     /locus_tag="VMU21_00405"
     CDS             7750..8772
                     /locus_tag="VMU21_00405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO76012.1"
                     /translation="MQIKKILFIRRDNIGDLICTTPAIHAAREKFPDARIGILVSTYN
                     ADVIKNNPDIDEVYIYEKSKHAPDKNRLSVWWDNLKVFQKIRRERYDIAIGCGTYSIR
                     LARYTALTGAKRRVGYVPRDVRKSRWYNAPVPEPDRPAHEVETVFHLISALGIHGPLP
                     PMRVFPLEKETQKVRDFLTAAGIKRDKPLVAFHISSRRQQNRWPIEKFIDLARDILSR
                     KEAEIMLLWSPGSKKNVYHPGDDEKADLFVRSVQPGPAAYRTTAVRELIAAISVSDLV
                     VCGDGGAMHIAAALGKRIVTIWGSSGPIRWRPWGVTHLILQDESRRAENISVQSVIDA
                     VQKLLT"
     gene            8916..10220
                     /locus_tag="VMU21_00410"
     CDS             8916..10220
                     /locus_tag="VMU21_00410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HUO76013.1"
                     /translation="MHIGIIGTGYVGLVTGACFAEFGVFVTCVDKDEKKIRSLRKGIV
                     PFYEPGLEDLVQRNIKQGRLKFTTKIADVVDDCLVIFIAVGTPPRGDGSADLQYVDVV
                     AEEIGRHMNGYKVIVTKSTVPVGTGKRISRIISKNQRAKDNFDIVSNPEFLREGAAIE
                     DFMRPNRVVIGANSQQAVAILKDLYNPLYLIETPFVITNIETAELIKHASNSFLAAKI
                     SFINEIANLCEKVGADVNMVAKGMGLDRRIGPKFLHAGPGFGGSCFPKDTRALLRLAE
                     EHDTELSVVKAAISANEKQKGIMFDRIRRSMGPLKGKVVAVLGISFKPNTDDLREAPS
                     LYLIEKFLKDKARVRAYDPAAMDNAKALFPQVAYCKDPYDAAKGADALVIVTEWNQFR
                     NLDLERIKGLLRKPFFFDLRNIYEPEKVKRLGFKYFCVGRCT"
     gene            10358..10432
                     /locus_tag="VMU21_00415"
     tRNA            10358..10432
                     /locus_tag="VMU21_00415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10390..10392,aa:Val,seq:cac)
BASE COUNT         3008 a         2384 c         2819 g         2850 t
ORIGIN      
        1 aagggatatc atgtccttct tgatgcgatc ccgttagtct tgaaaaaggc tcctcatgcg
       61 atattcgcct ttgtgggtga tggcccacag aaggaaaata tttctggtac aatagagcag
      121 ttggggttat cggggaatgt gcgtctgctc ggaatgagat gggatatccc taatgtgctg
      181 aagtccatag acatttttgc tcttcccact cttcaggaag cccttgggag tgcatttgta
      241 gaagcaatgg caatgggtaa gccggttatt ggaacaaacg tggggggagt gagtgaagtg
      301 ataaaagacg ggagtaacgg ttatctggtg gagccaaaca atgccgctgc attaagtgag
      361 gcaattctga aggtgcttga agatccggaa agggggagga gtatgggaag ggaaggcaga
      421 agaatggtgg aggaactgtt caccaacgaa aagatgtgtg aaaaaatgca tgacctgtat
      481 atgtctctcc tccaagggaa acgttcatga gatccgtccc tgttttgatg tatcaccacg
      541 tgaatcctca taaaggagac atggtgaccg tgaccccaac aacctttgaa cggcagatgg
      601 aatatcttag cgaggcagga tatagaacct tgaccctcga tgaactgctc tcttatatta
      661 aaggggactt ggtcttacaa cagaaagcag tcgtcgtgac ctttgacgat ggctggctcg
      721 ataattatat ctatgcgttt cccgttttaa aaaaatataa catccatgcc tccatattta
      781 tcgttacaaa cattataggg cgggcctcac gggatggcgg aaagcccccc actgttgtgc
      841 cgactcaccg ggagtctaag ctaattatgc aaaaggggga agagcatgaa gtttttttgc
      901 ggtgggatat tatcaaagaa atggcagaga gtgaactcat cgatttcttc tctcacgcca
      961 aaacacatgc cagttgtgat ttcctctccg aacctgagtt gtccgaagag cttgaagcat
     1021 ccaagcatgt catagaagag aggctcaaaa aaccttgtcc cttcctgtgc tggccattcg
     1081 gcagatacac cagcttcgcg attgaggtcg cacgaaaagt ggggtacagc gcaacctata
     1141 ccaccaacca tggaattgtt actccagggt cggacccctt tgcgatcaaa agaattgggg
     1201 tgaaggatga tatgggatgg tttaagtcaa ggatgcgaat ctatagtaac ctgttgctag
     1261 ccaggatcta cctcaaattg aagaggaaat gagaataagc cctgctgatg gtctgctgtt
     1321 cctggtgatg agactgctga aatcccttga taagagaaag cgcgacgtga agtattttga
     1381 cccctcctct gtgaaccgta ttttggtggt ttcatcgacg gccataggag atacactatt
     1441 gtccactccc gcgatcaaaa ctgttcggga acggtatcct catgctgaca ttgtagccca
     1501 tttcaatatc aagaacatgg aactctttga taacaacccc catataaacg gcatcattcc
     1561 ctattatggg gggtacaaaa agttcttcgg gactgtcagt aagttccgga aacacaagtt
     1621 cgaccttgct ctcatattcc acggcaatga accccaggca tcgcccacgg cttacctttc
     1681 aggcatacgg ttcatcatga agctccccaa caacggtgaa tatcgtttct tgctgtcgaa
     1741 cacagaaccg gtgatgtcct ggcttgactt cgggcatgcg attgaacagc gactgaaagt
     1801 cgctgagctt gccgggtgtg tggcgagcgg actgccgcgt atggagcttc cgctgcgcgg
     1861 agagggcgag aacgccgttg atgcctttct ggatggaagc aacattactc cgtcagatcg
     1921 attagtcggt tttcaggtgg gagcttcaac actcagccga atgtggtttg ctgatcggtt
     1981 tattgaactg gggaggaggc tcattcatga actcggcgat attcaaattg tcatcaccgg
     2041 gtctcctcag gaatacggct attgcaagaa gatcgcagac gctataggca cgaaggcgtt
     2101 cgtgtcggca gggaagatcc ccttaaggta tgtcccttcc ttggttcgga gacttcgggt
     2161 gcttgtcacg ggtgacacgg gaatcatgca catggcgatt gccaccggta ccccaatagt
     2221 tgccctattt gcagtttcag attcacagag gtcaggacct tactatgaca aggaaaagca
     2281 cagggttata cagaaggaaa agacatgcga tccctgcacg agcaagaaat gcacgtacca
     2341 gcggtgtatg gagaacatca gcgtagagga ggtcttttgt gaactgatgg aggtaatcga
     2401 gagatgagca atcaactgtg cccggtctgc aagggaatca ctgccccctt ttttgtgggg
     2461 aaaagagacc ttttggagtg tatccattgc catattgtat ttgatacgcg agtttcgtac
     2521 gataggaatt tttacgagca ccagcgggca gcttcggtgg acgctaccaa gatagacgcg
     2581 aggcggagga atgtcagaca aaggataaat cttataaaaa cactcttgca taaagacatt
     2641 tcgctccttg atatcggctg tggcgagggc gtgttccttc gggaggtgct cgcggatgtg
     2701 cgcgaagcaa caggccttga gccgactctt ttttacgtta actatgctcg tgaacacttt
     2761 aacctcgatg tccggcaggg gcttattgag gacgttgatt ttcccgatgg ggcattttcg
     2821 gttatcacaa tgtttcacgt gattgaacac gtggacgacc ccgttgcagc actgaagaaa
     2881 ataggtcggt ggctcaaaac gggaggacat cttgtgatag aagtccccaa catcaagagt
     2941 cctactgcga gatacaaggg tctggattgg gagatgataa tccctgagca taggtttcag
     3001 ttctacgggg aatcgttacg gtatgtactt gacagggctg gatttcgtct catttctctg
     3061 cgaacccgtg atttcgatca ataccggaca agcatcggaa agaacctccg caaactcggt
     3121 ataggcacgc aagagtacag gccaaaagcc ccctcagtcg gacatgaggt gcgtgtacca
     3181 ggaattcctg gtggaccgat atctccggca aaaaggattc gcaggttcat tcaagcacca
     3241 cttaaagccc ttttgggatg gcttgtcatg aaattggcac gcggcgatta tctttttgtc
     3301 gtggctagaa aggcatgata tttacgtggg taaagggttg gtatatggtg aggtgcctgg
     3361 agaataacac agtggcagtt ttctcatgca attgtcttcc gacaaaaaga tggcggagga
     3421 attattgttg atactcagga gatttcagtg acaattatac cgaagcacat actcatactt
     3481 gatactggca aggagtgggg tggagggacg aacagcctcc tggaacttct ggggagaatc
     3541 gataggaaag cctttcgatt taccggagta ttttatcacg actatcaaaa ggggaaggaa
     3601 tctacgataa aaactgagct ggagaaaatg ggaatcccgt tcatgctcct aaagcaagaa
     3661 aaacagcctg ttatggccaa gttccttaaa gagctactca ggtgtatgtt cttttttagt
     3721 aagaagttaa gaagatacct tgtattttca gtcgactacc atgtgaggat caaaccgaat
     3781 gcggaaaaaa tcgttgagat cttgaaaaat ctcaaggctg ctctgcttta tctgaataac
     3841 cagccgtcat cgaacctgga agggattctc gctgcccaac aggtcggcgt ccccatcatt
     3901 cagcatgcga ggagcaatgc ttcactttta cccgctgagg tgaagatgat taacaggaac
     3961 attacctcta tgatatgtgt gtccaacggg ctcaaagaaa attacataaa acagggcgta
     4021 ctgggaaaca ttgtcacagt tgtgcataat ggtatagatg tgacaagaga accggaagtc
     4081 ccccgcgaga tagtcctgag gagccttgat gcgggaaaag atgaagtagt cgttggcatg
     4141 gtcggctcac tcatgaagag gaagggtttt gatatattta taagagcgct cagacatctg
     4201 aaagacgcgg gcgcacagtt taagggcgtt atcataggtg acggtcctga aaaagaacat
     4261 ctcagtcgtt tggttgagaa attcaacctg acagaaagaa tagtgtttac aggtttcagg
     4321 gctgatggac tctcttacat caatgccctt gatatccttg tcctctcatc gcctgaagag
     4381 ggattcccga gggttatcct ggaggccatg cttatgaata aacctgtagt cgtgtcggat
     4441 atacctgggc cttcggaact ggtggtacat aatgagaccg gatatcttgt cccggccgga
     4501 caacccagtg cctttgctga ttgtctcaga cggcttatag cacagcctga aatgagaaag
     4561 caaatgggga aaaaggggag ggagagagta attgaacact tctcggttga gaaatacgtt
     4621 catggtgtag acagtgtgtt taaaaaggtg ctgggattaa agaaatgaca gcggaaatgt
     4681 ccaatacaca cgaatcaatt ccatggagcg atatcaaggc caggcgtgaa gaagtcaaaa
     4741 attcgtatcc cactctcttt accatccctc ttatgtactg tacgaagagg gaactcattg
     4801 gtaatgtggt gaaaaccatt gaggggaagg tgctcgatat cggtgccgcc gatagatttg
     4861 tgcgggatat ctgtgctcag gtaggcaagg agattgaata caggagtatg gatgtggaca
     4921 gaacaggatt ccatgagtat tactccctcg atgcggttca ggaaacgttt gacgcaatct
     4981 ttctcctcga tatgattgaa cacatatcat tacgtgaagg aatattattg ctgcagaggt
     5041 gtaaagaaat gctgaatcct gacggaagaa ttattctcac cctcccgaat aataatcacc
     5101 ccactgcctt tgcttcagat tgtacccatg tgacatcata tcgttaccat gaagtggggg
     5161 gaacactgtt agcggcagga tttgaagagt tgcagatatt caggatctca gccaaaagaa
     5221 gactcaaaca ccggcttctg gcaactcttc tcacgcctgt tctgaaattc ctggacatgg
     5281 attttgcccc gggaattttc ctcactgcaa aaaggaaata gactatgctt tatatagtga
     5341 taacatatct ggcagccccg gtcttatatg tcctgatact cctgcgcaga agaaaacaga
     5401 taaaaaacat cctcgtcatt cagacagcaa agattggtga tgtgatttgt tcaaccccgg
     5461 ttttcagaga gataaagaag ggatatccgg aggctcattt gacggtaatg gcaactcctg
     5521 tcacccttga actcttagag attaatccgc atgttgatga gatcataacg atgaatcaca
     5581 aagactataa gggaccttcg ggcaaactta ggctggccaa tctgctccgc aaaaggaaat
     5641 ttgacatcgc ggtatgcctt aaccctaacg tcctctttgc gtgcgcaaca ttctggggac
     5701 tcgtaccagt ccgactttct gtgatgcctg atttctccgg aagcaccttt aaacttgcag
     5761 cctttttttt tagctctctt gaaagacatg tgaaaggccg attggtcatt gaaacgtatt
     5821 tgaggatgtt gagagccatc ggaattgaaa gtaataacat ttccaaagag gtctataaat
     5881 cagaaaccgc ggagaacagg gtgagccagc gtattggcga gcttaagaag cctttgatag
     5941 gcatagcggt cagcagcggg aacaagctga aggagatggg accagagaaa attatacagc
     6001 ttgcagcgat actgttggac accaccgatg tcttcatcgt gctcatcggg tcttcccagg
     6061 acagaggtac ttcctcttta atacgaaaag cccttgggaa aaaggagaga atcctggatg
     6121 taacaggaga gttcggtctc ggggaactcc cggcccttat tgaacgcctt tcgctccttg
     6181 tcggcgtgga tacgggcatt gtatacatgg cagacgcatt atccgttccg gttatagaca
     6241 tagccggacc ctcagatatg gaagaccagc gacccaccgg tgcaaagagc atgattcttc
     6301 agaagaaggt ccaatgcgta ccctgttctc atgcctttca tgccccctat gcatgcgtgc
     6361 ggaatacacg tgagtgcatt caattgatgt cgatagatga aatctgctat gctattaaaa
     6421 aattttttcc ggaagtgcac tgtccataat gaacattctc aataatctca ctctatctca
     6481 tggcgtacgt ttttttctct atgcgttcat atttcttcag cctgtgaatc gcttcaattc
     6541 ctctagggag atagcctttg gcggcctttt ggtattcttt gtccttaaat tattcaaggg
     6601 cgggctgaaa gatatacggt tgagtgatgg aacaatcgtt gccttatttt tattaaccgc
     6661 gtggtcttta atggtctctt tattgggacc atatcccgtg gatagcctga gtgcaatgaa
     6721 gaataacctc cttgcccaga tcctcatatt ttttgccatc atcacagagt tcaaaacatt
     6781 acgggaactc aatggactct tttggatcgt aatctctagc tttttacttg tcactcttat
     6841 ttcagtattt gaagtcgtta atctcacaaa ctatgatcag ctattgaaaa gtcatgatat
     6901 gtttacgcat aaaatgttca tcggtgggta tgcaaatcac gcgaccttct atcttccctt
     6961 cattcttgcg tggctgatcg cgtttgatga acctcgggtg aggaggtggg tggggctggg
     7021 aacactcata acaggaattg tggtagtgtt cgtgtattca cacagaactg cattgattgc
     7081 cttaccgagt gcttttttca ttatacttct cctcaccaga aagtataaaa ttctccttgc
     7141 tgcctgcctc ttcgtcatta ttgctgccgc tgcaataggg ttgtcaaaat ccgatgtact
     7201 ctcacggtat aaatcccttg gaaatccaaa aacgtatatt acaaatagag gcgtaagcgg
     7261 ccggttcgca gtatgggaag gggcattaca gatcatcaag gagaggccga tcctcgggta
     7321 cggatatggg tggaaaaaaa tcgcatgggt tgcgcaggac ttaaatcttg ccgattcctg
     7381 gaagaaagat tttcctcaca tatatgcgta ttacgtcggc gaggcgcact tagggtatgg
     7441 cagggtcaat cctcacagcc ttgcacttca gatccttttt gaaatcggag tagtcggcct
     7501 tcttattttt ttgtggttct gggcgactat tattcataaa atcattaagg ttggattttc
     7561 aaaggaacac tcggactcaa ggagctatgt gctctgcagc gtgggggttc ttgcttcata
     7621 tctcatcatt aacattccca atggtttctg ggaagactcg tacggcagag tgatcttctt
     7681 ttttattgcc tcaatttttg ttgctttccg acagcatgtc aacggactca ctcctcaaga
     7741 atcattcaca tgcaaataaa aaagatcctc ttcatccggc gtgacaacat cggtgatctt
     7801 atttgcacca ccccagcgat ccatgcagca agagagaagt ttcctgatgc gaggatcggg
     7861 atactggtaa gtacgtacaa tgcggatgta atcaagaaca acccagatat agacgaagtg
     7921 tatatctatg aaaagtcaaa gcatgctccg gataagaaca gattgtctgt ttggtgggat
     7981 aatctcaagg tttttcagaa gatacgcaga gaacggtacg atattgctat agggtgtggc
     8041 acttattcga tccgactggc gcgctatacg gcgctgaccg gggcgaaaag gcgtgtcggg
     8101 tatgtaccca gggatgtccg taagtcccgc tggtacaatg ctcctgttcc ggagcccgac
     8161 cggcctgccc acgaagtcga gacggtcttt cacctgatct cagccctcgg tatccatggc
     8221 cctcttcccc ccatgcgggt atttcctctg gaaaaagaga cgcagaaagt ccgggacttc
     8281 ctgactgccg cgggtatcaa gagagacaag cccctcgtcg cctttcacat cagcagcagg
     8341 cgtcagcaaa atcgctggcc tatagaaaaa tttattgact tagcacgaga tattctcagc
     8401 cgtaaggaag cagagatcat gcttttatgg tccccgggga gcaagaagaa tgtctaccac
     8461 ccgggagatg atgagaaggc cgacctcttc gtgcgctcgg tgcagcccgg tcccgcggca
     8521 tacaggacca ctgccgtgag agaactcata gcagccatca gcgtcagcga tctcgttgtc
     8581 tgcggtgatg gaggcgccat gcacattgcc gcagcattgg ggaagcgcat tgtgacgata
     8641 tggggatcct ctggccctat tcgatggaga ccctggggag taacacatct gattttgcag
     8701 gatgagagca ggcgggcgga aaacatctct gttcaatcag tcattgacgc tgttcagaag
     8761 ttgctcacat aagaaagctt cgcggggaaa ctcctgtgtc agcgctctcc agtggttttg
     8821 gggagtttac atcaggggat gcttctgcca taataaaaaa cctgatgatc atggcaggga
     8881 ggatatctcc tggagaaaga ggataagggg taatcatgca tatcgggata ataggaacag
     8941 ggtatgtcgg tttagtgaca ggggcatgtt ttgcagaatt tggcgtgttc gtcacctgtg
     9001 ttgacaagga tgaaaagaag ataagatctc tcaggaaggg gatcgtcccc ttttatgaac
     9061 cgggacttga agaccttgtc cagaggaaca tcaaacaggg aaggcttaag ttcaccacaa
     9121 agatcgcgga tgtggtggat gactgtctgg tcatttttat cgcagtgggg acgcccccga
     9181 gaggggatgg ttccgctgat ctccaatacg tcgatgtggt ggcagaggaa atcggccggc
     9241 atatgaatgg ctataaggtg atcgtcacca agagcaccgt ccccgtaggg accgggaaga
     9301 ggatctcgcg gatcatatca aaaaatcaga gagcaaagga caatttcgat attgtctcaa
     9361 atccggaatt cctcagggag ggggctgcca tagaagactt tatgcggccc aacagggtgg
     9421 tgatcggtgc gaacagtcag caggcggtcg cgatactcaa agacctttat aatcccctct
     9481 accttatcga aacccctttt gtgatcacca atatcgagac tgcggaactg atcaagcacg
     9541 cctcaaactc ctttcttgcc gccaagatct cttttatcaa cgagatcgca aacctctgcg
     9601 agaaggttgg cgctgacgtg aacatggtag ccaagggcat ggggcttgac cggaggatag
     9661 gccccaaatt tcttcatgcc ggacctggtt tcggcggctc atgttttcca aaggatacaa
     9721 gggcacttct tagacttgcg gaggagcatg ataccgagct ttctgtggtc aaggcagcca
     9781 tttctgctaa tgagaagcag aaaggcatca tgttcgacag gatcagaaga tccatgggcc
     9841 ccctgaaggg caaggttgtt gcggtcctcg ggatatcctt taagccgaat acagatgatc
     9901 tgagggaagc accgtctctg tacctcatcg agaagttttt gaaggataag gcgagagtaa
     9961 gggcttacga ccccgcagcc atggataatg caaaagccct gttccctcag gttgcttact
    10021 gtaaagaccc gtatgatgcg gcaaagggag cggatgcatt ggtcattgtg accgaatgga
    10081 accagttcag gaaccttgac ctcgaaagga taaagggact gctcaggaag ccttttttct
    10141 ttgatctaag gaatatctac gagccggaaa aggtgaaaag actcgggttc aaatatttct
    10201 gtgttgggcg atgtacgtaa ttaaatgcga caaatccttt atatcataaa ttggatttta
    10261 agtgtggaga tgatgattta ttcggattaa taacttgacg atgtgttttt tttgttttat
    10321 tattcaccaa tcactattca ctgataactg atgtgccggg cgattagctc agtgggagag
    10381 cactgtcttc acacggcagg ggtcgctggt tcgaaaccag catcgcccac catatgacat
    10441 gaagggtggt tgagtagtat gaaaccagct atcaaataaa gacttctcct tacacgggag
    10501 ctcgcagtgc gagtccaaca tcaaggggtt agccatttat ggctagctcc tttttcatgg
    10561 gattgtgcag ttgacctgcc attaatggct gaattaagaa tatccacagc ctttacctta
    10621 tggctcggtg cgaagtgggc ataccgtacg tcatagtaaa ggttttatgc tccaggagtt
    10681 ctttaacagt ggtaatatca accccagcca tgacaaggta agatgcaatt gaggagaccc
    10741 tgacactcct ttttggagag attgaaacga agacgtttca aacctgcaaa gacagagctt
    10801 cttacgtgaa aaaatcaata cattagaagc tgtattctcc aaagcatgta gaaaaaaaac
    10861 ctacaaaaga atcagataaa ggcatatact tattaatgtc atttacggga aaattcgggt
    10921 acgcggccct tcctcaaaga gacgtgaaaa tgcctcttaa cataatttaa ggaggataaa
    10981 atgaaaatga aacagggatt caaaatagtt ttagcactgt ttctgtgttt tataatgtct
    11041 gtggcattca tgaattgtgg c
//
LOCUS       DATKOU010000012         5328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4250073, whole genome shotgun sequence.
ACCESSION   DATKOU010000012 DATKOU010000000
VERSION     DATKOU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250073"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VMU21_00420"
     CDS             <1..921
                     /locus_tag="VMU21_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HUO76014.1"
                     /translation="DDAIVLFADPTKEMHIVALTAKFLEQNTSKNDDANAYVGVFDYR
                     TKEFGVSFDVTYVDHQNSFYNSDGSPTTVANTANGGLPVNPLAAGVDTHLWNFGIRGD
                     VTLFGFNIYADGEGQTGKITNLAPQNEKYKGYAVLVGASYKLDPVKFLLEWGYGSGDD
                     NPTDGSFKEYVTSLSAYQRDTYIYGYRSVSAGNVLGIPVKSGGLANTNRIRFGVDADL
                     TKELFANVTYYYLRANKLVPAWTSAGVDASHSIGSEIDWLFKYKIDKNLTYYVEGGYL
                     FAGNFYKVVTPGNVSPDNAWAIRNGLQLTF"
     gene            1240..2022
                     /locus_tag="VMU21_00425"
     CDS             1240..2022
                     /locus_tag="VMU21_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HUO76015.1"
                     /translation="MSRAVEIEDLGASLNGREILTDINLTLPEGRFLGIVGPNGGGKT
                     TLLRIILGLVKRASGRLQVFGKTPKDAAREGLFGYLPQHMNVDTSFPATSLDVVLMGL
                     YGKGGAFRRISKNDKNRAGDMLKTMGMAGYEDHIYGRLSGGQQQRVSIARALMSEPRL
                     LILDEPSTGIDVVGQEDFYHLLKGLQKKFILSIIMASHDIGVITAYVDEIACLNRTLH
                     YQGSPLGALNEEVLKELYGKSMEIIMHSHLCEKCERLQPEGK"
     gene            2026..2955
                     /locus_tag="VMU21_00430"
     CDS             2026..2955
                     /locus_tag="VMU21_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HUO76016.1"
                     /translation="MPRLFREIQDSRTLYKDKRIACNGFFSDQRLLNPDPGALEVGGM
                     TEILSYGFMQRAFIAGIAMAFFAGVISTFIVLRRVSFLGSGISHAAFGGVAIGFFSGI
                     NPLTTALIYCIAVAFAIEIISTKGKLAEDTAIGVFFSGSMAMGVVLISLSKSYTIDLF
                     GYLFGSILAISNEETYMAVGMAAAGMIVFSLIMKDLLFITFNEELAIINGIPVRLIKA
                     CFLLSMAVVIVIGIKIVGIVLISALLVVPGATAKLLGQKFYGMILISAIVAVISTVSG
                     LVISYVYDLASGGTIVMVLVLFFGIAFLFRKKT"
     gene            3045..3452
                     /locus_tag="VMU21_00435"
     CDS             3045..3452
                     /locus_tag="VMU21_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76017.1"
                     /translation="MGEKTKPGLNFTLLCDDVRQEMGGKISLMGIFENVYAGSFPAVH
                     PRLATVNEWADGLGEFEATLRILSPDRKTVLRETVTKLKLVNARFKHRDISIHLNLEF
                     KEPGTYWIENYLDGILVNSVPLNVMLVKEKSFH"
     gene            3465..4007
                     /locus_tag="VMU21_00440"
     CDS             3465..4007
                     /locus_tag="VMU21_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HUO76018.1"
                     /translation="MPEGGDRQKLIEEKIGHAEAKASMGLTLIRQVIRDLLLNEKGYS
                     EEDMEVDRQFEIIQDNSRVSVSVDYILTLSGKRVMVIKCSPGALESRERHIVSFARVV
                     DSYQIPFAVVTDGLHARILNAESGKLISEGLDSIPSRSQATEILQSTELKPYPAGRMD
                     REKRILLAFDVIKCTQESCE"
     gene            complement(4010..>5328)
                     /locus_tag="VMU21_00445"
     CDS             complement(4010..>5328)
                     /locus_tag="VMU21_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO76019.1"
                     /translation="EGQSFITTLLMVSTGKTLISDDSRQRLKKLKEMREVRVFVSPTC
                     PYCPQLVLYAVSAAIEKRDSVSAEVIEIYENRDLAEKYGAMSVPKAFVGDTLIAPGLQ
                     PEEYFMESLVEGKPVEYVMPAGREDLRDYDIVIIGGGPAGLTAAVYAERSGLKSIIFE
                     KGNVGGQIALTPIVENYPGFSSIPGNTLVELMAKQAMEYSPVLQGVGVSEIIKRDGGF
                     EIATGRGTYTAKAIIIATGAYSKGLDAPGEKRFAGRGVSYCATCDGYLFKDGKSVLVV
                     GGGNSALTDALYLESLGAHVTLVHRRDSFRGEERLQQSVFQRNIPVLWNSRVAEILGK
                     KLVEKARIEDVMTGKTRDIKTEGIFVAIGYEPNNEIAKKLGLTMVAEGYIKVDEKMRT
                     SMPLVYAAGDITGAVKQIVVAVSQGSVAALTAFEDIANPYWKEKRA"
BASE COUNT         1281 a         1390 c         1337 g         1320 t
ORIGIN      
        1 gatgacgcga tcgtgctttt tgcagatccg acgaaagaga tgcacattgt tgccctcacc
       61 gccaagttcc tggagcagaa cacctcaaag aacgatgacg caaatgccta tgtcggtgtc
      121 tttgattaca ggaccaagga gtttggtgtg agctttgacg ttacctatgt ggaccaccag
      181 aatagcttct ataacagtga cggctcccca accaccgttg cgaacacggc aaatggagga
      241 ctcccggtta atcctcttgc tgccggcgta gacacccatc tctggaactt cggcatcagg
      301 ggcgacgtca cattgttcgg cttcaacatc tatgcagacg gtgaaggcca gacaggaaag
      361 ataacgaatt tggctcccca gaacgaaaag tataagggct atgcggtcct ggtaggcgcc
      421 agctacaagt tggatcctgt gaagttcctc ctcgagtggg ggtatggcag cggtgatgac
      481 aatcccacag acggcagctt caaggaatac gttacctcgc tctctgccta ccagagggat
      541 acctatatct atggctaccg gtcagtgagt gcaggcaacg ttctcggaat tccggtcaag
      601 agtggcggcc ttgccaatac caacaggata aggttcggtg ttgacgcaga cctcacaaag
      661 gagctctttg cgaatgtgac ctactactat ctccgtgcca acaagctcgt gccggcatgg
      721 actagcgcag gtgtagatgc cagccattca atcggtagtg aaattgactg gctcttcaag
      781 tacaagatag acaagaacct aacctattat gttgagggcg gctatctctt tgcagggaac
      841 ttctacaagg tcgtaacacc tggcaatgtg agccctgaca atgcatgggc aataaggaac
      901 ggactccagc tcacattcta aattgtcctg agcatattgt gtccgaaagg gcgggtcatg
      961 ctgacccgcc tttttttttc tgcgtgagca ctgcagcctt tctcgaatat cacaccactc
     1021 acagccaata agaacttcgg tgaagttgaa tttctttctg acccacacta taatgggatt
     1081 accgaaaacc atgaaaacgg tctcttggta tatccccatg cctcctccct ctgcccccgg
     1141 tcaggaatcc gcatgtacgg ggaatcgtcc atgaaaagtg aaagacccgg aggtaatgat
     1201 gatcgtttcc gtgacccgca atgagaaggg gtgtatgaaa tgtcccgtgc agtagagatt
     1261 gaagaccttg gagcaagcct taatggcaga gagatactca ccgacattaa tctcaccctc
     1321 ccggagggaa gatttctcgg gattgtaggt cccaatggag ggggaaagac gacgcttctc
     1381 cggataatcc tcggccttgt caaacgggct tcaggcaggc tgcaggtttt tgggaaaaca
     1441 ccgaaggatg cggccagaga gggactgttt ggatacctcc cgcagcatat gaatgtggac
     1501 accagctttc cagccacttc cctggatgtg gtgctcatgg ggctctatgg aaagggaggc
     1561 gcattcagga gaatctcgaa gaatgacaag aacagagcag gggatatgct taagacaatg
     1621 ggaatggccg ggtatgaaga ccatatctat ggacggctct ccggaggtca gcagcagagg
     1681 gtttccattg cgagggccct tatgagcgag cccaggctcc tgattctcga tgagccgagc
     1741 acagggatcg atgtggtggg ccaggaggac ttttaccacc tgcttaaggg acttcaaaag
     1801 aaattcattt tgtccatcat catggcctcc catgacattg gggtcatcac tgcatatgtt
     1861 gatgagattg cctgtctgaa caggaccctc cattaccagg ggagccccct gggtgccctt
     1921 aacgaggagg tgctgaagga gctctacgga aagagcatgg agattatcat gcacagccat
     1981 ttatgcgaga agtgtgagag gctccagcca gaagggaaat gatatgtgcc gcgacttttc
     2041 agagagatcc aggatagtcg tactctctat aaggacaaga gaattgcttg taacgggttt
     2101 ttctctgacc aaagactgct taacccggat cccggcgctc ttgaggttgg aggaatgaca
     2161 gagattctct cctacggctt tatgcaaagg gcattcattg caggaatcgc aatggccttc
     2221 ttcgcagggg tgatatccac cttcattgtc ttacggaggg tatccttcct gggctcagga
     2281 atatcgcacg ctgcctttgg cggcgtggct ataggatttt tttccggaat caaccccctg
     2341 accactgcgc tcatctattg catcgcggtc gcattcgcca tagagatcat cagtaccaag
     2401 ggaaagcttg cggaggacac cgccatcggg gtgttttttt ccggctcaat ggccatgggg
     2461 gtagtgctca taagcctctc aaaaagctat accatcgacc tcttcgggta tctttttgga
     2521 agtatactcg cgatcagcaa tgaagagaca tacatggctg tcggcatggc ggcagcaggc
     2581 atgattgtct tttcccttat tatgaaagac ctcctgttca tcacattcaa cgaagagctc
     2641 gccattatca acgggatccc ggtcaggctc ataaaggcgt gcttccttct ctccatggcc
     2701 gttgtcattg tgatcggcat aaagatcgtg gggattgtcc tcatctccgc ccttctggtg
     2761 gtacccggag ccaccgcaaa gcttctcgga cagaaattct acgggatgat tctcatctca
     2821 gccattgttg cggtcatctc cacggtatcg ggactcgtga tctcctatgt gtatgatctc
     2881 gcctccgggg ggaccatcgt aatggtactt gtgctctttt ttggtatcgc attcctcttt
     2941 cgaaagaaaa cgtagtcact tcatgattct cttttcactt ctctcacatc tccctcgccg
     3001 cacagcaggg aatactcttc cctccatttc atgtataata gcgcatggga gaaaagacaa
     3061 aaccgggact taacttcacc ctcctctgtg atgatgtgag acaggaaatg ggaggaaaga
     3121 taagcctcat ggggattttc gagaacgtgt atgccggtag tttccctgcc gttcatccta
     3181 ggctggccac cgtcaatgag tgggctgacg gcttgggaga gtttgaggcg accctcagga
     3241 ttctgtcacc cgacaggaag actgtactga gggaaaccgt gacaaagctg aaacttgtga
     3301 acgcaaggtt taaacacagg gacatatcca tccacctcaa tttagagttt aaagagccgg
     3361 ggacgtactg gatagaaaac taccttgacg gtattcttgt gaattccgtc cctctcaatg
     3421 tgatgctggt aaaagagaag tccttccatt gaaaggctgt tgcgatgcct gaaggtggtg
     3481 accgccaaaa actcatcgaa gaaaaaatcg gtcatgcaga ggccaaggca tccatgggcc
     3541 ttaccctcat cagacaggtt atcagggatc tgcttctgaa cgaaaaaggg tattccgaag
     3601 aggacatgga ggttgacagg caattcgaaa ttatccagga taactccagg gtctcagtat
     3661 cggttgatta tattctcacc ctcagtggca agagagttat ggtgataaaa tgttctccgg
     3721 gagctctgga atcaagggaa cggcatattg tttcttttgc acgggtggta gattcatacc
     3781 aaatcccttt tgcagtggtg actgacgggc tccatgcgag gatactgaat gcggaatcag
     3841 ggaagctgat ctccgaaggt ctcgattcca ttcccagccg atcacaggcc acggagattc
     3901 tccaatccac tgaactcaaa ccctatccgg ccgggaggat ggacagggaa aagaggatcc
     3961 tcctggcctt tgatgtcatc aagtgcaccc aggagtcctg tgaataggat tatgctcttt
     4021 tttccttcca ataaggattg gcaatatcct cgaatgcggt gagggctgca acagagccct
     4081 gggagaccgc taccacaatc tgttttacgg cacccgtgat atcacctgcc gcatacacca
     4141 agggcattga ggtcctcatc ttctcatcca ccttaatata gccttctgca accatcgtga
     4201 ggccgagttt cttcgcgatc tcgttgttcg gttcatagcc tatggcaaca aatatccctt
     4261 cagtctttat atcccgggtc ttcccggtca tgacatcctc tatcctggct ttctccacga
     4321 gcttctttcc gagaatctcc gccacacggc tgttccacag cacagggatg ttcctctgga
     4381 aaacgctctg ctggagtctt tcttctcccc tgaaggagtc ccttctgtgc acaagggtta
     4441 catgggcgcc gagactttca agatagaggg catccgtcaa ggcactgttg cctccgccca
     4501 ccacgaggac actctttccg tccttgaaaa ggtatccatc gcatgtggcg cagtaactta
     4561 cgccccgtcc cgcgaacctc ttttctcccg gtgcgtcaag ccctttgcta taagcacccg
     4621 tggcgataat gatggccttt gccgtgtagg ttcccctccc ggtggcgatc tcaaagcccc
     4681 cgtctctttt tattatctca ctcactccca ctccctggag gacgggagag tattccatgg
     4741 cctgttttgc catgagttcc accagagtgt tccccggaat gctcgaaaaa ccaggataat
     4801 tctctactat cggggttaag gctatctgtc cccccacgtt tcctttttcg aatataatgc
     4861 ttttgaggcc gctcctttct gcgtagaccg cggcagtcaa tcctgcaggg ccccctccta
     4921 taattacaat gtcatagtcc ctcaggtcct cccttcccgc aggcattaca tattccaccg
     4981 gtttgccctc cacaagggac tccataaagt attcttcggg ctggagtccc ggggcaatga
     5041 gggtatcgcc cacaaaagcc tttggcactg acatggctcc gtacttctcc gcaaggtccc
     5101 tattttcata gatctcgatg acctctgccg ataccgaatc ccgcttctct atggctgcag
     5161 agactgcgta gagaaccagc tggggacagt agggacatgt ggggctcaca aagaccctca
     5221 cctccctcat ctccttaagc ttcttcagtc tctgtcgcga atcatccgaa atcaaggtct
     5281 tcccggtgga caccataagc aatgtcgtaa taaaggactg gccttcct
//
LOCUS       DATKOU010000013         6357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1171490, whole genome shotgun sequence.
ACCESSION   DATKOU010000013 DATKOU010000000
VERSION     DATKOU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171490"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..1319
                     /locus_tag="VMU21_00450"
     CDS             387..1319
                     /locus_tag="VMU21_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HUO76020.1"
                     /translation="MYDERFLLHRTGPEHPEVPERLLAAYRGLEEAGLISRLTMVKAK
                     RARQEWIEDVHSIKYIMNFEEACVLGMPDFNHPDCQISRESFETAMLAAGGIVDTVRL
                     VWEGKLDNAFCAVRPPGHHAEVNKAMGFCFFNNIAIAARYLKRECGVKRIGIIDFDVH
                     HGNATQHIFEDDPTVFFYSIHEHPSFSFPGTGREFERGTGAGYGFTLNSPVLPGKGDA
                     EYKELIEKELIPAFEEFKPEFILVSAGFDAHVEDDMSDINLTTECFSWIMERIVEIAD
                     KYSHGKLVSVLEGGYALKRLPELVRNHVKILLGE"
     gene            1367..2047
                     /locus_tag="VMU21_00455"
     CDS             1367..2047
                     /locus_tag="VMU21_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO76021.1"
                     /translation="MFIDKYMSKDVVTVGQDFGILEARELMVKHRIRHLPVVEADNTL
                     IGIVSDRDMRSIMPSQLLATQEYEKEKGLIMDVKVRDVMTKNPVTISPMDTLEDALLL
                     MQKMRVGAFPVVDSQKKLQGIVSIRDLMRTFINMLGIEEPGTLLCILAENKQGTMKKI
                     VDAITEEKVSFGSILVARHWEEGKRAFFPYLLTNNVAPIKRKLRELGFTLLDPLEWCL
                     DLTPKPEE"
     gene            2234..4213
                     /gene="acs"
                     /locus_tag="VMU21_00460"
     CDS             2234..4213
                     /gene="acs"
                     /locus_tag="VMU21_00460"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HUO76022.1"
                     /translation="MADAKKIEVLQAEGRKFPPSKAFSEKAHIKSIKEYEALYKKSVE
                     DPEGFWAEMAEKHITWYKKWNKVLEYDFTKPQIKWFQGGKLNVSYNCLDRHITTATRN
                     KAAIIWEADDGSYKTFTYQQMYYEVNKFANVLKKHGVKKGDRVTIYLPMIPELAISML
                     ACSRIGAIHSIVFGGFSAQSLKDRIQDCNSSMLITSDEGMRAGRFVALKSNADAALNE
                     CPTVKKVIVVQRTKSGEIHMQNRRDVWWHTEMSAPDIKLFCEPEQMDAEDPLFILYTS
                     GSTGKPKGVLHTTGGYLLYTNLTFKWIFDYHEEDIHFCTADIGWVTGHSYIVYGPLSA
                     GATSLMFEGVPTYPNPGRFWDICDKHQVNIFYTAPTAIRALMREGESWVNKHELTSLR
                     VLGTVGEPINPEAWMWYHIHVGKEKLPIVDTWWQTETGGILITPIPGAMTLKPGSATR
                     PFPGVVPRVLKEDGSVAGADEGGYLVIEKPWPGMLRGTYGDPENKRMKEVYFTRFPGK
                     YFTGDGARVDADGDYWLMGRIDDVINISGHRLGTAEVESALVSHEAVAEAAVVGYPHD
                     IKGEGIYVYVTLKEGVNPTDELKKILVGHVRTVIGPIATPDKLHFAPGLPKTRSGKIM
                     RRILRKIAHGQVEDLGDTSTLADPSVVDNLVKGRQ"
     gene            4333..4884
                     /locus_tag="VMU21_00465"
     CDS             4333..4884
                     /locus_tag="VMU21_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HUO76023.1"
                     /translation="MDKFFLTKRDTVLVVVDIQERLAAVMSERQKVIGNSLHLIEAAK
                     VLHFPVLLSEQYPKGLGPTVPEIKGALPSYEPIEKLMFGCCGEPMFMEAIASIGKRSV
                     ILCGMETHVCVLQTCLGLIREGYAVHVVEDAICSRSKENYRIGTEFMRDAGAVITGTE
                     TVLFQLLEKAGTEEFKILSRRIK"
     gene            4979..5521
                     /locus_tag="VMU21_00470"
     CDS             4979..5521
                     /locus_tag="VMU21_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76024.1"
                     /translation="MKSFLIILLCIVMVGSLILVKGSDALETPLRTEWVNVVDVTGTF
                     TLILYGGNYFNDVHTIAFLDLEGDQYTFEPYAPEFEYRVVKGLSAKEALDKAQDFVST
                     IGNCFSRSQLSKVLDQNGKTIGYEMRPFYNPICYGLSDVLDVSYWNKGGKVVIRIKLY
                     PAVEKQLQSGDGSQGRDSGR"
     gene            5828..6271
                     /gene="smpB"
                     /locus_tag="VMU21_00475"
     CDS             5828..6271
                     /gene="smpB"
                     /locus_tag="VMU21_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HUO76025.1"
                     /translation="MRIVCQNKKAYHDYSIEETLEAGIQLQGTEVKSLREGKANLRDS
                     YVLLKNSEAFLLNCHISPYSHGNIMNHEPLRTRKLLLHRKEIERIRGKAQQKGYALIP
                     LKIYFKDSFAKVEIGLARGKRLYEKRETIKEREAKRTIERAMKSG"
BASE COUNT         1746 a         1422 c         1712 g         1477 t
ORIGIN      
        1 acaacgagta acaaaagctc ttcagtcggc ttgaacaatg ctgattgaaa tcattgacga
       61 aaacaccggg ccaagaggcg gctgccgaag cgcttacggc tatctttgct cgtatcccgg
      121 aggcccaatc atatctgatc agcggatttc gtgagtgatg catttatgca ggcagccctt
      181 tcagagcctt cacggcaatc gatgacgtca acagagctta acggaggtaa gggagataat
      241 gtataagtgg atgatcgact aagtgagcaa ctgccgcatg atgcgctgag ggacttgaca
      301 cctgcggaat atattggaca taatgctgaa gccactgctt tgaaactgtc tgagtaatga
      361 ggaagccaac gagacgaaca ggctttttgt acgacgaacg ttttttgctc catcgcaccg
      421 gacccgagca cccggaggtg ccggagcggc ttctcgctgc gtacagaggg cttgaagagg
      481 caggcctgat ttctaggctc acgatggtta aagccaagcg ggcgcggcag gaatggatcg
      541 aggacgttca cagcattaag tacatcatga acttcgaaga agcatgtgtc ctgggaatgc
      601 ctgatttcaa tcatccggac tgtcagattt ccagggaaag tttcgagacc gccatgctcg
      661 cggccggtgg aatcgtagat acggtccgtc tcgtctggga aggcaaactc gacaacgctt
      721 tctgtgctgt gaggcctccg ggacatcatg cagaagtaaa caaggccatg ggtttctgct
      781 tcttcaataa cattgccatc gccgcgagat acctcaagag agagtgtggc gtgaaaagga
      841 tcgggattat agattttgat gtccaccacg gcaacgccac gcagcatatc tttgaagacg
      901 atccgaccgt atttttctac tccattcatg agcatccatc gttttctttc cccggaaccg
      961 gacgggaatt tgaaagggga acaggcgctg gttacggatt caccctgaat tctccggtct
     1021 tgcctggcaa gggagatgct gagtacaagg aactgatcga aaaagagctg ataccggcgt
     1081 ttgaagagtt taagcccgag tttattctcg tttccgcggg gtttgatgca catgttgagg
     1141 atgacatgtc ggacataaat cttacgacgg aatgcttctc atggattatg gagaggattg
     1201 tggagatcgc ggacaagtat tcgcacggaa aactcgtctc agtacttgaa ggaggttatg
     1261 cgctgaaacg cttgcccgaa ctggtacgaa atcatgttaa aatcttgctc ggtgagtgat
     1321 ttttgttgaa atactttttt gacgcgtaaa gcggggggga tagaatatgt tcatcgacaa
     1381 atatatgagc aaagatgttg tgacggttgg tcaggacttc gggatcctcg aagcacggga
     1441 gttaatggtg aaacaccgta tcaggcatct tcctgtggtt gaagccgaca atacccttat
     1501 cggcattgtc agcgaccgtg acatgcgaag cataatgcct tcacaactgc tcgccacgca
     1561 ggaatatgaa aaagagaaag ggctgatcat ggacgttaag gtcagagacg tcatgaccaa
     1621 aaaccccgtt acgatttccc ctatggatac ccttgaggat gccctcctgc tgatgcagaa
     1681 gatgcgcgtc ggagcctttc ctgttgtgga cagtcagaaa aagctgcagg gaattgtttc
     1741 catcagagac ctgatgcgga ctttcatcaa catgctcggg atcgaggagc ccgggacttt
     1801 gctttgtatt cttgctgaaa acaaacaggg gacaatgaaa aagattgtgg atgctatcac
     1861 cgaggaaaaa gtatctttcg ggagcatcct tgttgccagg cactgggaag aggggaaacg
     1921 tgcgtttttt ccctatctct tgacgaacaa tgttgccccc atcaaaagga agctgagaga
     1981 actggggttc actctccttg atcccctgga gtggtgtctt gatctgactc ccaaacctga
     2041 agaatagggc ttaagtgttg ttaagctttc ggtaaaaaaa tgctttttct gagcatcttg
     2101 gggaggggtg aattatatcg atcgaagacc tgatctttat ctttaatcct cgggattgca
     2161 cagagaccgg ctatccaacg agtgaaggct aatacctaag catgaattaa aaaaataagg
     2221 agagacagaa ggaatggcag atgcaaagaa gattgaggtc ttgcaggcgg agggcaggaa
     2281 atttccccct tcaaaggcct tcagcgaaaa ggctcacata aagagcatca aggaatacga
     2341 agctctctat aagaagtccg tagaagaccc tgaagggttc tgggctgaaa tggctgaaaa
     2401 gcatatcacc tggtataaga agtggaataa ggtccttgag tatgatttta ccaagcccca
     2461 gatcaaatgg ttccagggag ggaaactcaa cgtctcctat aactgtctgg accgtcatat
     2521 aaccacagcc acaaggaaca aagcggcaat catctgggaa gcggatgacg ggagctacaa
     2581 aacattcacc taccagcaga tgtactatga ggtgaacaag ttcgcgaacg tcctgaagaa
     2641 gcacggagtc aagaaggggg acagggtcac catctatctt cccatgatac ctgagctggc
     2701 catctcaatg cttgcctgtt caagaatagg ggcgatccac agcatcgtct tcggagggtt
     2761 cagcgcacaa tccctgaagg acaggatcca ggattgcaat tcatcaatgc tcatcacctc
     2821 tgatgaaggc atgagggcag ggaggttcgt ggccctcaag tccaatgcgg atgcggccct
     2881 taatgaatgc ccaacagtga agaaggtgat tgttgttcag aggacaaaga gcggcgagat
     2941 acatatgcag aacagaaggg atgtatggtg gcatactgag atgtcagcac cggacataaa
     3001 actcttctgc gaacctgaac agatggatgc ggaagacccc ctcttcatcc tctatacatc
     3061 aggctccaca ggcaagccga agggagtgct ccataccacg ggaggctatc tcctctacac
     3121 aaacctcacc ttcaagtgga tattcgatta ccatgaggaa gacattcatt tttgtaccgc
     3181 tgacataggg tgggtgaccg gccacagtta tattgtctat ggcccgcttt ctgcaggcgc
     3241 tacaagtctt atgttcgaag gtgtgcccac atacccgaat cctggaaggt tctgggacat
     3301 ctgcgataag caccaggtga atatcttcta caccgctccc actgccataa gagcgttgat
     3361 gagggaagga gagagctggg taaataagca tgaactcacg tccctgaggg ttctgggtac
     3421 tgtgggagag cccataaacc cggaggcatg gatgtggtat cacattcatg tgggaaagga
     3481 gaaacttccc attgtggaca cctggtggca gacagagaca ggaggaatcc tgattacccc
     3541 tattcccggc gcaatgaccc ttaagccggg ttccgcgacc aggcctttcc cgggagtggt
     3601 tcccagggtg ttaaaggagg acggctctgt agcaggggca gatgaaggcg gataccttgt
     3661 gatcgagaag ccatggcccg gcatgttgag gggcacgtac ggagaccccg agaacaagag
     3721 gatgaaggag gtttacttca cgaggttccc cggcaagtac ttcaccggtg acggcgcaag
     3781 ggttgatgct gacggagatt actggcttat gggaaggatt gatgatgtca tcaatatctc
     3841 cggtcaccgt cttggcaccg cagaggtgga atcagccctt gtgagccatg aggcagttgc
     3901 agaagctgcg gtggtaggct atccccatga cataaaaggc gagggtattt atgtctatgt
     3961 gacgcttaag gaaggggtca accccacaga cgagctcaag aagattcttg tgggacatgt
     4021 gcgtacggtc atagggccca tagcgacccc tgataaactc cattttgcgc cggggctgcc
     4081 caagacaaga agcggcaaga tcatgagaag gatattgagg aagatcgccc acggacaggt
     4141 ggaagacctt ggggacacct cgaccctggc agatccctca gtggttgaca acctggtcaa
     4201 aggaagacaa taacagagaa gatcttacgg gaaaatgcag ccaagaagca gtaacgggga
     4261 aggggccatc ccctttcaca ggggatgccc cttctttttt atccggtagc acactaattc
     4321 tgagaggaca ctatggataa attttttctc actaagagag ataccgttct ggtggttgtt
     4381 gacattcagg aaagactcgc tgcagtcatg agcgaaagac aaaaggtgat tgggaactca
     4441 ctccacctta ttgaggcggc aaaggtcctt catttcccgg ttctcctcag cgaacagtat
     4501 cccaagggac tcggtccgac agtccctgaa atcaaggggg cccttccttc gtatgaaccc
     4561 attgagaaac tcatgttcgg ctgctgcgga gagccgatgt tcatggaggc tattgcttcc
     4621 atagggaaga gaagcgtcat tctctgcggt atggagaccc atgtctgtgt gctccagacc
     4681 tgccttgggc tcatcaggga gggctatgcc gtccatgttg tcgaggacgc catctgctcc
     4741 cggtcaaagg aaaactacag gatcgggacc gagttcatga gagacgcagg cgcggtgatc
     4801 acaggcacag aaaccgtatt attccagtta ttggagaagg cgggaactga ggagttcaaa
     4861 atactgtcaa ggagaataaa atagagacat ccgtgttggg accctgaagg aattacttat
     4921 aggtctttgc aggagacctt cccttttgcc ttcttctgtt ttgtggtata ctgaaaatat
     4981 gaaaagcttt ctgatcatat tgctttgtat agtgatggtg ggttctctta ttctggtcaa
     5041 gggctctgat gccctcgaaa cccctctcag gaccgaatgg gtaaatgtgg tggatgttac
     5101 gggaacattt accctgatac tttatggtgg gaattatttt aacgatgtcc ataccatcgc
     5161 atttcttgac ttagagggtg accaatatac ctttgaacct tatgcgccag aattcgagta
     5221 tagggtcgta aagggccttt cagcaaagga agccctcgac aaggcgcagg actttgtgag
     5281 caccataggt aattgttttt cacgctctca gctgagcaaa gtgctcgatc aaaacggtaa
     5341 gaccatcgga tacgagatga gaccctttta taatcccatt tgctatggcc tgtctgatgt
     5401 tctcgacgtg agctattgga ataagggcgg caaggttgtc ataagaatta aactgtaccc
     5461 agctgtggag aagcaacttc agagtggtga tgggtcacag ggcagagaca gcggacgtta
     5521 atccccgtgt cacggtacat ggaaagtgtt atccctcttc agcagttgcc tcacgttgaa
     5581 gccgctgtct ttcaatgaaa aatcaatata ctctctacca ctgccagtaa ccttgacgac
     5641 atccatagaa tcaggaagtt ctggcctttt ctaccccttt cgatttctgt aagcctcaag
     5701 gagtgacccg ttccagggtt tcaggatagg gttctcttcg catagtaagc tcacattccc
     5761 aagatttcca tacggtatca ggctttccaa gacctgataa agttatgaaa caatggtaca
     5821 atgagacatg cggattgtct gtcagaacaa gaaggcatac catgattaca gtattgaaga
     5881 aaccctggaa gcgggtatcc aacttcaggg cacagaggtg aagtcactcc gggaaggtaa
     5941 agcaaacctg agggatagct atgttttgct caagaattcc gaggcgtttc tcctcaactg
     6001 ccatatcagt ccctattctc acgggaatat tatgaaccat gaacccctga gaacgcggaa
     6061 actcctcctc cacaggaaag agattgagcg aatacgggga aaggcccagc agaaagggta
     6121 tgcgctgata ccgctgaaga tatactttaa ggactcattt gcgaaagtcg agataggact
     6181 tgcaagggga aagagactgt atgaaaagag agagacaata aaagagcggg aagcaaaaag
     6241 gactatagaa agggcgatga agagcgggtg atatgcgata tatgccatgc aatccttaca
     6301 gagaggggag gcattcgcct ctcagcaaga cattagccct ttggtaccag attaagg
//
LOCUS       DATKOU010000014         9683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3480659, whole genome shotgun sequence.
ACCESSION   DATKOU010000014 DATKOU010000000
VERSION     DATKOU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3480659"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..953
                     /locus_tag="VMU21_00480"
     CDS             330..953
                     /locus_tag="VMU21_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76026.1"
                     /translation="MVVPEGKRVMLRGETKGKLFSKGGEVIEFFVDGKSLGSTLSGGD
                     GLAFRQFVPPGPGLYHVTARSGNEKGKGVILALRKKTGIVFIDLEGSVLGGPFSGRSM
                     SGSRKVIKEINRRFPVVYIQTGFAGIKMLKEWLKENGFTPSPVVAWNNGAIFDDIYER
                     GLTIKAIIASPHIIQSAMKYHPLAFSFQSADDAVEVKDWEEIRKRLK"
     gene            1263..3167
                     /locus_tag="VMU21_00485"
     CDS             1263..3167
                     /locus_tag="VMU21_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HUO76027.1"
                     /translation="MITAEIKKKLPYVFLIAILGIGLVISVVAFTVVKKWEGQKLWME
                     FYQASENRQASLQRQIDSDLQALVSLQAFYLHSEKISRAEFSSFTKILLLQHTSIQAI
                     EWIPRIPYYQREQFEKAARKEGFVDFQITEQRAQGKVMRADRREEYFPVYFVEPYGSN
                     EITPGFDLASNPTIKESLEQSRDTEELVATAPSLVGQKSIGRPGFPSVFWVFAPIYAK
                     DALTDSVQARREHLRGFASGFFRLGDIVRGSLEYLESAGIDFYIYDQSPSKDDRFLYF
                     HQSRTRNRAISPMSDQEMKSFKGIKYSNTLNVAGRAWMVVYIPTPKFIAARQSWQPQY
                     VLSVGLVLTGLMVSYLWFNIRRAEELSKLNKQLLNEIVERKSAEEALQEYRAHLEDLV
                     SERTAELSRVNERLEQDIAERKKITEKLRESEERFRRIFEDGPLGMMISDLHYSTLKV
                     NKAFCEMLGYSEQELLERNIEDITYPEDVEKSVKLSKQVLRGKIPLFSIEKRYVKKTG
                     ETLWVNLTATTIRDQEGNVLYAMGMVEDISERKAVEHEKELLISELQDALANIKTLKG
                     LIPICAWCKKIRDDKGYWKKVETYIEEHSDASFTAGICPKCLHKEDPATYEEVFKDEK
                     EPGVSKIEQD"
     gene            3425..4225
                     /locus_tag="VMU21_00490"
     CDS             3425..4225
                     /locus_tag="VMU21_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HUO76028.1"
                     /translation="MKQGPLRIGKINYTNLFPIFSMLEKVADCSAYEFSEGVPSVVNG
                     LLREGKIDVSPSSSIEYLRRETDYTLLDGHSISSFGPIRSILLFSTTPIEALNGLTVL
                     TTSQSETSVALLDITLRKFYGFDCPLKSSHEPLSEAVKSYSAYLLIGDDALREVRRRP
                     GLHVYDLADIWYTHTGLPFVFALWIARNDCCMNAPLLLERFRKDLDEAKGRALKNLKA
                     IARESPLIPVLSEEELVSYWKGISYDLNDEHKKGLQLFRKYAAELKLI"
     gene            complement(4253..4852)
                     /locus_tag="VMU21_00495"
     CDS             complement(4253..4852)
                     /locus_tag="VMU21_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HUO76029.1"
                     /translation="MRFADEESKFPWLSMLLDAYAIIDEGVAAAIQEREGKSGAMLAC
                     RKGCDNCCRTHKDIPLYPLELVGVYWYCTEKMAKPLRELLRIQLKIHRAGDPCPFLIH
                     GACSIHVIRPVACRQFNVFTKPCGVGEDPYFTRRDDVLTPIQEYTDRAFSSMLPYYGV
                     THKGDRQKAIAHIIHTQAVNLQSFEWKKLVRLMDNFDSR"
     gene            complement(4943..5557)
                     /locus_tag="VMU21_00500"
     CDS             complement(4943..5557)
                     /locus_tag="VMU21_00500"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HUO76030.1"
                     /translation="MRRKIKLLSVMLCFVIASCAVITVNIYFPEKDVKEAYKVLEKEL
                     MDTDKKPAEKPEGKPQSSLGFEFVPSLYAQESGLADKISEIVKKMPDVVEAYRQMGAR
                     IADTDRLRDSGGVGEGMSGLLVEREGSLSPADKKIVEMENANRKTVMNGMARAIIRIN
                     RVPENEENLRQVVPQATQQFAAIRRDSAKKGWWVQDSNGNWSRK"
     gene            complement(5576..8773)
                     /locus_tag="VMU21_00505"
     CDS             complement(5576..8773)
                     /locus_tag="VMU21_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HUO76031.1"
                     /translation="MTVRRKRILRIISVTFLLFVIVFSALSYFRYLDLKKALLLKISY
                     KATSLFGQGVAIGDISFSPPGGITLHEIAIRNPEGFPEGKLLSIKRLHFDIRLMELFR
                     GLLSFRNIEIYSPELTLVLDKNKKLNMAEELRQFLLSKGTASYRIDSFTVHSGNVDIP
                     LGFFPGVRDINLSIKNLSSLQDTKTLINGTMAFAGNPVQFEGSIYLNNPSMNFAVSFT
                     SEEFGFSEFQEMLKGYPVDAGKVTFNFRVNAEGDTLHGCRILSSVRLKKGGLSDMRME
                     GDVFFDLKDHSVAIRELSWYDGDVRSVRLSGQVTDISGSPSYKADIQIPGIDLSHYHF
                     IKDVKMSGIITSDHLEVKGKGGESMPEISGSVRLTKGTIKSARLEIQESNADLVFSAD
                     KEMSLSAEVSAKVLRIKNMIAKPADAVLSVKAKGNPESMAIYSSLHLSPLSVRMGENR
                     SFSSEGLAFTFDGTAEKGRSFQGKVLVEMKNLTSADYRVPDLNGSLTMEYRDDLITTR
                     DVKVSAGETRASAGSVVIHMKGQKAGYWLEGKDIYAAYPHEEAEIKRTDFRLNVRTAA
                     QKLSGEIAFSFGGMIFRGVPSEGISGTAQFNEKEFSIHIPDAKVAGGKASLRAKGKVS
                     EGPFPIQGEMTAEHVDLGILSRAVSGVPAIPFSVSGILTRASFNGSAELPLSLNGRAS
                     VEAEHLSLLKKEDGKTLVKDVGVRTDLTCKGKDCEFTTDMAAGHVETRSSGMIRGLAE
                     KDREIMIKATLPETKITDIRNSMWDVFPDGLLYARLDGTLSSEALVNYRESGINMSGD
                     LRLKEVMLEGENSEYSVGPINGTVPIRYQNTAHDAVEIPSFERSEFANLEKYYSQEKV
                     PQTSHGITIGSLRYGFPLLDTIDIRIEPQGNVLKIDRISANIFGGRMYGSALVDISEG
                     VSYRAGILMELVSLTRLCGAIEPIRGYISGKVDGIATIKGSGMGLSHLIGKAEFWTYS
                     SGGEKTKISKEFLQKIGGTSLKTYLGDRSFSKGVMNLYLQNGFVIFRELEISNRNLLG
                     MNDLSVKVAPLNNRIGIDHLMWTITEAAQRAKSKE"
     gene            complement(8931..9293)
                     /locus_tag="VMU21_00510"
     CDS             complement(8931..9293)
                     /locus_tag="VMU21_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO76032.1"
                     /translation="MVIEDSLYTRREIRDILEGEGYTIIEAEDGVKGIQMATTEAPDC
                     ILLDLIMPDIDGIKILKLLHEQRPQIPVIVVSAHGQEAVRTQCLELGASACINKPPSA
                     DELRYTVQKVLVSRRKRS"
     gene            complement(9344..>9683)
                     /locus_tag="VMU21_00515"
     CDS             complement(9344..>9683)
                     /locus_tag="VMU21_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76033.1"
                     /translation="MGFDVTATRGTAEHLRRKGIEVGVVNKVTEGRPHIVDLIKNKEI
                     SFVINTVTGAQAQKDSFSIRESALQYRVPFTTTLSGANAVVNAIEKLLKGKIHIKSLH
                     EYHRSEKSLP"
BASE COUNT         2437 a         2407 c         2294 g         2545 t
ORIGIN      
        1 aggaaaggag tatgcttgtc aaggaaaaaa gtcaattccc cgggatatta ccacatattg
       61 taggatggtg atttctatga cgctatatat acgttttttt ccttcttgga ctacttcttc
      121 agcacgagcg aagcatagcc taaaagtact gcgccctggt tccgcatagt ttcctgtcaa
      181 aagctgttta aaaggaggtt cctgttgatt tatgatgtaa caaaaaaggc attcacgtca
      241 tggcgaggat tttaagggca tttattatct tgactcttct gttttgcatg tttcccctgg
      301 gtgctttgtc tgaggtaatt gtacatgaca tggtggtacc cgaagggaag agggtcatgc
      361 tcaggggaga gacgaaagga aagctcttca gtaaaggggg cgaagtgatt gagttctttg
      421 tagacggaaa atcgctgggg agtacccttt cgggtggaga tgggctggcc ttcaggcaat
      481 ttgtcccgcc cggaccgggt ctgtatcatg tcacagcaag atccggaaat gaaaaaggca
      541 agggagtgat ccttgccttg agaaaaaaaa cgggtattgt ctttattgac ctggagggga
      601 gtgtcctggg aggccctttc tctggacgtt cgatgagtgg aagccggaag gtcataaaag
      661 aaatcaacag gaggttccct gtggtctata tccagactgg tttcgccggc ataaagatgc
      721 tcaaggaatg gctcaaggaa aatgggttca cgccctcccc cgttgttgca tggaacaatg
      781 gcgcaatttt tgatgacatc tacgagagag ggctcaccat taaggcaatc atagccagtc
      841 ctcacatcat ccagtcagca atgaaatacc accccctggc cttcagcttt caaagcgctg
      901 acgatgcggt ggaggtgaag gactgggagg agatacggaa acggcttaaa taagacccta
      961 gccttacaat aaggccgcct tgcctttgtg ccttttcctt tcccggccct gctgacataa
     1021 tacctaacct gaatcgtaat ttgacaatta cgcccatccg tgataaaaat taatcaggag
     1081 aggaaaaatc ctgacaagac caaacctttc gaggagggga cgcaatgcgc cgggtctcac
     1141 aataagatgg ccgaactgcc gaaaggcaga gttcttggaa tgctcggccc ttttggtgag
     1201 tttttagaaa actgttttca ttcgaccggg gccttcaagg gtctgatcga acttaagaga
     1261 atatgattac tgccgaaatc aagaaaaaac tgccttatgt ctttctgata gcaatactag
     1321 gcattggatt ggttatttcc gtagtagcct tcactgtggt aaagaaatgg gaaggccaaa
     1381 agttatggat ggaattttat caggcttcgg aaaaccgcca agcctcgctc cagagacaaa
     1441 tcgactcgga cttgcaggcg cttgtgtcgc ttcaggcatt ctaccttcat tcagagaaga
     1501 ttagcagagc agagttcagc agtttcacga aaattctttt gctgcaacac acgagcatcc
     1561 aggcaattga atggataccg cgcataccat attatcagcg tgaacaattt gaaaaggcag
     1621 caagaaaaga gggcttcgtc gattttcaaa ttaccgaaca gagggcccag gggaaagtga
     1681 tgagagcgga tagacgcgaa gaatattttc ctgtctattt tgttgagccg tatgggagta
     1741 acgagattac cccggggttt gaccttgcct caaatccgac gatcaaggag tctctggaac
     1801 aatcccgtga cacggaagaa ctggttgcca cagcgccctc actagtgggc cagaagtcta
     1861 ttggtcgacc cggttttcca agcgtttttt gggtttttgc cccgatatat gcaaaagacg
     1921 ccttaacgga ttctgttcag gcccgtcgag aacaccttag gggctttgct tcaggcttct
     1981 tccggctcgg tgatattgtg cggggatccc tggaatatct ggaatcggca ggtatcgatt
     2041 tttatatcta tgaccaatcc ccgtctaaag atgaccgttt tctttatttc caccaatcac
     2101 gtacgcgaaa tagggcaatc tcgccgatga gcgatcaaga gatgaagtca tttaagggca
     2161 taaaatactc caatacgctc aatgtggcag ggcgggcatg gatggttgtg tatataccga
     2221 cacctaagtt catagcagcg aggcaatcgt ggcagcctca atatgttctc tcggttgggt
     2281 tggtgttgac cggtcttatg gtgagttacc tgtggtttaa catcaggcga gcggaagaac
     2341 tgtcaaaatt aaataagcaa ctcctgaatg aaattgtcga acggaagagt gctgaggaag
     2401 cattacagga ataccgggca cacttggaag atttggtaag cgaacgcacc gctgaactat
     2461 caagggtaaa tgagcgtctg gagcaggaca tcgctgagcg caaaaagata acagaaaagc
     2521 tgcgagagag cgaggagcgg ttccgcagga tttttgaaga tggtccgctc ggcatgatga
     2581 tctcagacct ccattacagc acccttaaag tcaacaaggc attctgcgag atgctggggt
     2641 actcagaaca ggagctttta gagcgcaata tcgaagacat tacctatccg gaagacgtgg
     2701 aaaagagtgt aaagttatca aaacaggtgc tcaggggtaa aatccccctt ttttccatag
     2761 agaaacggta cgtcaaaaag accggagaaa ctctctgggt caatcttacg gctacgacca
     2821 ttcgcgatca agagggaaat gtgctctatg ccatgggcat ggttgaggat atcagcgaac
     2881 gaaaggctgt tgagcatgag aaggaacttc ttatctccga gctccaggat gcattggcga
     2941 acatcaaaac attgaagggc ttaattccta tatgcgcctg gtgtaaaaaa atacgtgatg
     3001 acaagggata ttggaaaaaa gtggaaacat atatagaaga gcattcagac gcctccttca
     3061 ctgctggtat atgcccaaaa tgtttgcaca aggaagaccc agctacttac gaggaagtat
     3121 ttaaagacga aaaggaacca ggagtctcca agatagagca ggattgacat tactgccaaa
     3181 agtaattact gccatcaata tttctgctgg aatcgtcagc cttttcaaaa agtgagaact
     3241 gttgagtgat cggtgacctt tttccaaaga agtggaaaag tggacaccca attaagttcc
     3301 atttaggagt ccttcagatt gttcggggac aggtgtccgg aacagagtat aatgagtgtg
     3361 tcctgaatca cctggagacg catctgtggt agaatatctc acttgataag caaagggata
     3421 ttgcgtgaag caggggcctc tcaggatagg aaaaatcaac tatacaaacc tttttcccat
     3481 cttctccatg ctggagaagg tagcggactg ctcggcctat gagtttagcg agggagtacc
     3541 ttcagtcgta aacgggttat tgagggaagg gaagattgat gtgagccctt catcgtccat
     3601 cgaatatctc aggcgcgaaa ctgactacac ccttctggat ggacattcca tcagttcttt
     3661 cgggcccatc aggagcattc tccttttcag cacaacacca attgaagcac tgaacggatt
     3721 gactgtgttg accacttccc agtcagagac ctctgttgcg ctccttgaca tcaccctaag
     3781 gaaattctat ggtttcgatt gtccactgaa atcttcccac gagcccctct cggaggctgt
     3841 gaagtcatac tccgcgtatc ttctcatcgg agatgatgcc ttgcgtgaag tccgcaggcg
     3901 gccgggactt catgtgtacg accttgcaga tatctggtat acacataccg gcctcccctt
     3961 tgtctttgca ctgtggattg caagaaatga ctgttgcatg aatgcaccgc tcctcctcga
     4021 gcgtttcaga aaagacctgg atgaggcaaa gggccgggca ttgaaaaatc tcaaggcgat
     4081 tgcacgtgaa tcacccctta tcccggtcct ttctgaggaa gagctggtct cgtactggaa
     4141 aggcatatcc tatgacctta atgacgagca caagaaaggc cttcaacttt tcaggaagta
     4201 tgcagcagag ctgaaactga tctgacctgc tattcctctt acactcggaa cgtcacctgg
     4261 aatcaaaatt gtccatgagc cggacgagtt tcttccactc gaaggactgg agattgacgg
     4321 cctgagtatg aataatatgc gctatggcct tctgtctgtc gcctttatgg gtaacgccat
     4381 agtaagggag cattgaggaa aaggcccggt cagtgtattc ctgtatcggc gtgaggacat
     4441 catcccttct ggtgaaatag gggtcttctc ccacgccaca aggtttggtg aagacattga
     4501 actgcctgca agcaacagga cggatgacat ggatggagca cgcgccgtgt atgagaaagg
     4561 ggcacgggtc tcctgcgcgg tggatcttta gctgtatcct gagaagttcg cgcaggggtt
     4621 tggccatttt ttccgtgcag taccaataca cacctaccag ttcaaggggg tagaggggaa
     4681 tgtctttatg ggtgcggcag cagttatcac agcctttcct gcacgcaagc attgcaccgc
     4741 ttttcccctc cctttcttgt atggctgcgg ccacaccctc atcaatgatg gcgtaggcat
     4801 ccagaagcat tgatagccac gggaatttcg attcttcatc tgcaaaacgc acacgcctca
     4861 aatcgcatcc ttcatctctc ccttgaggtt tactcatccc gatccgtcat atcgtgcttc
     4921 gtggtactct acatcactct cttcacttcc tgctccaatt gccgttggaa tcctggaccc
     4981 accacccctt ctttgctgaa tcgcgcctga tggcggcaaa ctgctgggtg gcctggggaa
     5041 ccacctgcct gagattctcc tcgttttccg gaaccctgtt gatccgtatg attgcccttg
     5101 ccatgccgtt catcaccgtt tttctgttcg cattctccat ctccacaatt ttcttatcag
     5161 caggggataa tgacccttcc ctttccacaa gcagtccgct catcccttcc cccactccac
     5221 cactgtctct cagacggtcg gtatcagcaa tcctcgcacc catttgcctg tatgcctcca
     5281 ctacgtcagg catctttttc acaatctccg aaatcttgtc tgccagtcct gactcctggg
     5341 catagaggga cggaacaaac tcgaacccaa gggaactctg cggcttgccc tccggttttt
     5401 ccgcgggctt tttatcagtg tccataagct ccttttcgag gactttatac gcctctttca
     5461 catccttttc aggaaagtag atatttacag tgattactgc acaggatgca atgacaaaac
     5521 acagcatgac cgaaagtagc ttgattttcc gcctcatgat ttatcctcct ttttgttatt
     5581 ccttgctctt cgccctctgg gcagcttctg ttatggtcca catcaggtga tcgattccga
     5641 tgcggttgtt aaggggtgca actttgacag acagatcgtt catacccagg agattcctgt
     5701 tcgatatctc gagttcccta aaaatcacaa acccattttg aagatagagg ttcatcactc
     5761 ccttactgaa actcctgtct ccgagatagg tcttcaggga agtccctccg atcttttgga
     5821 ggaattcctt gcttatcttc gtcttctcgc ctccggagct gtaagtccaa aactcggcct
     5881 tgccaataag atgggagagg cccatgccag aaccttttat cgttgcaatg ccatccacct
     5941 ttcctgagat atatcccctt atgggttcaa tagccccgca gagccttgtc aggctgacta
     6001 gctccatgag aatccctgcc ctgtagctca caccttcgga gatatccaca agggcagaac
     6061 catacatcct gcctccgaat atgttagcac taattcggtc tatcttcaaa acgtttcctt
     6121 ggggctcaat ccggatatcg atggtgtcca gcagcgggaa tccatagcgg agagagccga
     6181 tggtgattcc atgagaggtc tgtggcacct tttcctggga ataatatttt tcgagattcg
     6241 caaactcaga acgttcaaaa gaggggattt caacagcatc atgtgcggtg ttttggtacc
     6301 ggatgggaac cgttccgttg attggtccta cggaatactc actgttctcc ccttcgagca
     6361 tgacctcttt aagccgtaaa tccccggaca tatttattcc gctctccctg taattgacca
     6421 atgcctcaga agaaagtgtt ccgtccagtc ttgcatagag aagtccatca gggaacacat
     6481 cccacatcga atttcttata tccgttattt tcgtttccgg aagcgttgcc tttatcatga
     6541 tctccctgtc tttttccgca agccccctga tcatcccgga actccttgtt tccacgtgcc
     6601 ccgcagccat gtcagtggta aactcacaat ccttcccttt gcacgtgaga tccgtcctca
     6661 ccccaacgtc cttcacaagt gtcttgccgt cttccttctt cagaagtgag agatgctctg
     6721 cctctaccga tgcccttcca tttaatgaga gggggagttc tgcactccca ttgaaggagg
     6781 cacgggtaag aattcccgat actgaaaagg gaatggccgg gactccggac acagcccgtg
     6841 agaggatgcc caggtcaaca tgctcggcag tcatctctcc ctggatagga aaaggacctt
     6901 ccgatacctt tccctttgcg cgaagagatg ccttgccgcc tgctaccttt gcatcaggaa
     6961 tatgaatgga aaactctttt tcgttaaact gcgctgtccc tgaaatccct tctgaaggaa
     7021 ccccccgaaa gatcattcct ccgaatgaaa aggcaatttc tcctgatagc ttttgggcag
     7081 cagtacgcac attcaacctg aaatcggtcc tttttatttc tgcttcctca tgaggatagg
     7141 cagcgtagat atccttacct tctaaccagt atcccgcctt ctgtcccttc atatgtatca
     7201 cgactgagcc tgccgaagcc cttgtttctc ctgcagagac tttcacatcc cgcgtagtga
     7261 tcaaatcatc cctgtactcc atagtgaggc tcccgttgag gtctggaact ctgtaatcag
     7321 cagaggtgag atttttcatt tccacgagaa ccttcccctg aaatgatctg cctttctctg
     7381 cggtgccgtc aaaggtaaaa gcaagaccct ctgaagagaa gctcctgttc tctcccatcc
     7441 ttacgctcag cggggaaagg tgaagagacg agtatattgc catactttcc gggtttccct
     7501 ttgccttgac cgaaagcacc gcgtcagccg gttttgcaat catattcttt atccggagaa
     7561 ccttcgcgga tacctccgca ctaagcgaca tctccttgtc tgctgaaaat acgagatccg
     7621 catttgactc ctggatctca agccgggcag atttgatcgt ccccttggta agcctcacag
     7681 agcctgatat ctcaggcatg ctctctcctc ctttcccctt tacctctaaa tgatcagagg
     7741 tgattatccc gctcattttt acgtctttta tgaagtggta atgagaaagg tctatcccgg
     7801 ggatctgtat atccgcctta tacgagggac tgcctgatat gtcagtgacc tgtccgctca
     7861 gacgcactga gcggacatca ccgtcatacc atgaaagttc acgaatggca acagaatggt
     7921 ccttaagatc gaagaagaca tctccttcca tgcgcatgtc actcaaccct ccctttttga
     7981 gacgcacact ggaaagaatc ctgcacccgt gcagtgtatc cccttctgca ttcactcgga
     8041 aattgaatgt gaccttcccg gcgtcaacag gatatccctt taacatctct tgaaactcag
     8101 aaaaaccaaa ctcttccgat gtaaacgata ccgcgaaatt catggaagga ttgttcagat
     8161 atatcgagcc ttcaaactgc acagggttgc ccgcgaatgc catcgtccca ttaatgagcg
     8221 ttttggtgtc ctggagagat gagaggtttt ttatggagag attgatatcc cttaccccgg
     8281 ggaagaaacc aagaggaatg tccacatttc ctgaatgcac ggtgaacgaa tcgatccggt
     8341 aactggcagt gcccttgctc aagagaaact gtctgagttc ttctgccata ttcagtttct
     8401 tattcttatc taaaaccaga gtgagctcag gggaatagat ttcaatgtta cgaaaagaga
     8461 gcaggcccct gaagagttcc atcaatctta tatcaaaatg gagcctcttg atgctgagga
     8521 gttttccttc cggaaatccc tcagggttcc tgatcgcaat ttcgtggagg gtaattcccc
     8581 ctggagggct gaatgagatg tccccaatcg caaccccttg gccgaagagc gaagttgctt
     8641 tataggatat ctttaagagc aacgcctttt tcaggtcgag ataccggaaa taggagagcg
     8701 ccgaaaagac tattacgaaa aggaggaagg tcacagaaat gattctgaga attctttttc
     8761 ttcgaactgt catgttctgt ttttctctgc aatgctcaga aacgcggcac aatggttaca
     8821 caacccgggt acgcggctcg cgctgcttcc tgcctatatt atcaactgtc attacgaaaa
     8881 ttccatcaga ttaaggagga ctccggataa aggggaaaat gccgtgttcg tcaggaccgc
     8941 ttcctgcggg agactaacac cttttggacc gtatacctca attcatccgc gctgggcggt
     9001 ttattaatgc aggcagacgc acccagttcc aggcactggg tgcgcaccgc ttcctgaccg
     9061 tgcgcagaga ccacgattac agggatttgc ggtctttgct catggaggag cttgaggatt
     9121 tttatcccgt ctatgtccgg catgatgaga tcaagcagaa tacagtcggg tgcttcggta
     9181 gtcgccatct ggattccctt aacgccatct tcagcctcaa ttattgtgta accctccccc
     9241 tccagaatat cccggatttc ccttcgcgta tataacgagt cttctatgac caagataagg
     9301 ggcatgcgtt caggaggtaa taccctgcag ggataccagt aagctaaggg agagatttct
     9361 cacttctatg gtattcatgc aacgatttta tgtggatctt tcccttcagg agcttttcga
     9421 tggcattcac caccgcattg gcgccggaaa gggtagtggt gaaggggacc cggtactgga
     9481 gtgcgctctc ccgtatggag aacgaatcct tctgcgcctg ggcgcctgtt accgtgttga
     9541 tcacaaagct gatctccttg ttcttgatca ggtcaacaat gtggggtctt ccctctgtca
     9601 ccttgttcac cacaccgacc tcgatcccct ttcttcttaa atgctctgct gttcctcttg
     9661 tggcggtcac atcgaatccc atc
//
LOCUS       DATKOU010000015         5417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4250835, whole genome shotgun sequence.
ACCESSION   DATKOU010000015 DATKOU010000000
VERSION     DATKOU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250835"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VMU21_00520"
     CDS             <1..1466
                     /locus_tag="VMU21_00520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUO76034.1"
                     /translation="LAALFWRVPEVIVDASRYFTQAKYLELYGIEFFVREWGKAILSW
                     TDLPLVPFLYGLIFKFFGESRLFIQVVTTLLFSATIVLTSLIGKELWDEETGLYGGIL
                     LLGIPYLFVQVPLMLVDVPTMFFFTLAVFAFIRALGSKGTATILVSSVSLFLAFLSKY
                     STWPMLSILVVIVFVFIKVQGQGSVRHTISRGVVTFLAAALLAGVVIFCRFEVFSEQI
                     KLLISYQRPGLGRWEESFISTFFFQVHPFITLSAIVSVWVAIRGRDPKYLIIIWLPLL
                     LMVFQVQRIRYTIPLFPMLTLMASYGLQQIGDKDARRFIASCIVMSSVVVALCAYLPF
                     TQKMSAANLRDAGGFLDTLDEKEIEVFTLLPKEPVVNPAVEVALLDLYTRKRIIYHGE
                     FGAAPGRDIISSPLRFTWEYKNPSYYAVKDKPCDGEMATVILSGEGGGDLPSALVQKL
                     KNYREVIKFNADEGVFEHKTFVSVYRQRVSESGEKRH"
     gene            1731..3155
                     /locus_tag="VMU21_00525"
     CDS             1731..3155
                     /locus_tag="VMU21_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HUO76035.1"
                     /translation="MADQENNTKGQIWAFIVTGIVILISLAYYKANVYYMYLVVYIWF
                     GFAYGMMLQYGRFCMASASRDLFAAGVPRMAVGIMVALMFFSIIQALLSATDMSTFHP
                     APFGIHTLISGTIFGVGMVLAGGCASGSLYKIGEGNGTSFLSIMGICFGQAIFSVVPW
                     FNSIIPQSWEISAKAKDLPVSLNASFDKYLAGYVWDQPKIQLSHTKVISETLPGVSKY
                     FLGDALISAIIPALLLLVLIYAVYIRKGFIKKRAKQKGGKTGLTDNLAGIWSMITASK
                     RTTLMGVLIGIVCGLHIFVMKGMQIKFGVSNFGELLTRMGHTADVSLNGTVFDPGYWY
                     ITSQEAQFGAWVMEKLGINMRDNIFFGVNNGIPELWRNPALWMSIGIILGAMVMSLMN
                     KEFKLKLPKGELIVWGLLGGTLMGIGSRPALGCNIGAFFIRVAGGDPSGWLFGIGMAV
                     GAFFGVKFFNWWSERKMAKEMADF"
     gene            3204..3455
                     /locus_tag="VMU21_00530"
     CDS             3204..3455
                     /locus_tag="VMU21_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HUO76036.1"
                     /translation="MAMKFEKTGEKTYLLDVCGYVCPHPQIYCKKSLEKIGEGDVLEM
                     VFDNPSSAETIIQMLDQGGHEVLEKKHEGGKIIFRIQKG"
     gene            3489..3671
                     /locus_tag="VMU21_00535"
     CDS             3489..3671
                     /locus_tag="VMU21_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76037.1"
                     /translation="MKAPMVILILIVAAALGFLLVFSQQKEKSPQAAQGQGAAGYGSA
                     ASGGGTVAPPSGADGK"
     gene            3708..4169
                     /locus_tag="VMU21_00540"
     CDS             3708..4169
                     /locus_tag="VMU21_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO76038.1"
                     /translation="MKRLLIAVDDTKGSKEAFKFCTEFCKCMLPEKIVLLYVERFEGR
                     SFMTEMLPDSELGTLKEVLQGTEYKEALDKRADEILNFYKDTLGDNGVTNVKTVKKIG
                     NPAEEILKTAKEEDVEMIVIGSRGKRVGHLLMGSVSREVANSSDIPVLVVK"
     gene            4310..>5417
                     /locus_tag="VMU21_00545"
     CDS             4310..>5417
                     /locus_tag="VMU21_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO76039.1"
                     /translation="MDEQGTKVVVLGGGLTGLSAGYLLSKAGNRVEVFERDSIVGGLS
                     KTMVQDGFRFDLGGHRFFTKDKKIDDFVKTLMEGELVSVSRKSKIYMRNRYFDYPLKP
                     LNALFGFGLPTTMKILGDYGLEKFKGIMREERSISLEDWVVQNFGRKMFDIYFKEYSE
                     KVWGIECSRISAEWVETRISGLSMAKAIKNALFSLNGKDLPTLVDRFLYPRLGIGRIS
                     ERLREEIERNNNVHTEADVQHIHHAGFSVKSIAVKTKNHLDLIQGEEFISSIPITKLV
                     AMLRPSPPEYILEAASRLRFRDLVVVAIMINRARVTDQTWIYIPEQKIPFGRIHEPTN
                     WSEKMAPAGKTILVTEFFSFAGDTLWSESDEKLAE"
BASE COUNT         1407 a         1098 c         1466 g         1446 t
ORIGIN      
        1 ttcttgcagc actcttttgg agggtgcctg aggtcatcgt cgatgcttca agatatttta
       61 cccaggcgaa atatcttgag ctatacggaa tagagttctt cgtcagggaa tgggggaagg
      121 ctattctttc atggaccgat cttcccctcg tcccgtttct ctacggcctg atttttaagt
      181 tctttggaga gagcaggctc ttcatccagg tagttacgac actcttgttt tcggcaacta
      241 ttgtgcttac ctcactcatc ggaaaagaac tttgggatga ggagacaggt ctttacggag
      301 ggatacttct cttgggtatt ccgtatcttt tcgttcaagt gccccttatg cttgtggatg
      361 tacccaccat gttcttcttc accttggcgg tatttgcttt tataagagca ctggggagca
      421 agggcacggc aacaatcctc gtctcttcag taagcctctt cctcgccttt ctctcaaagt
      481 actcaacatg gccgatgttg tccatactgg ttgttatcgt attcgtgttt ataaaggtgc
      541 aggggcaggg cagcgttcgt cacacaatct cgaggggtgt ggtgacgttt ttagcggcag
      601 cgctcctggc aggggtcgtt attttttgca gattcgaagt attttcggag cagataaagc
      661 tgctgataag ttatcaaagg ccggggcttg gcagatggga ggaaagtttt atttcgacct
      721 tcttttttca ggttcaccct tttatcaccc tgtcagcgat cgtttcggtc tgggtggcga
      781 ttcggggaag agatcccaag tacttaatta tcatttggtt gccactcctt ttgatggtct
      841 ttcaggtcca aagaatccgc tacaccattc cccttttccc tatgctcacg ctcatggcct
      901 cttacggact acagcaaatc ggggacaagg atgcgaggcg atttattgct tcatgcatcg
      961 tcatgtcatc tgttgtggtg gcgttgtgtg cctatcttcc tttcacacaa aagatgagtg
     1021 cagcaaacct gagggatgca ggaggctttt tagacaccct ggatgaaaag gagatagagg
     1081 ttttcactct gttgcccaag gaacctgtgg taaacccagc agttgaggtt gctcttcttg
     1141 acctgtatac gcgaaaaagg attatctacc atggggagtt tggtgcagcc ccagggagag
     1201 acataatttc gtctcctctt cgattcacat gggaatacaa gaacccgtcg tattatgcgg
     1261 taaaagacaa accctgtgat ggtgagatgg ccacggttat actgtccggg gaggggggtg
     1321 gagatctgcc ctctgctttg gtgcaaaaac ttaagaatta ccgtgaggta ataaaattca
     1381 atgcggacga gggagtattt gagcataaga cctttgtgag tgtgtatcgt caaagggtct
     1441 ctgaatccgg ggaaaagcgt cactagatct gatattgccc agtcaataaa taaacttata
     1501 tcgcaattag catactacac atattatgga tgaattaatt tttcttattt gacgattttt
     1561 gtgcttctgt attagtttta tgtggagctt atttgtgtag tctcattata ttgtaataaa
     1621 atataattca gatggggaaa ggggcaggaa cattacaatt cactgtcata cagggctgaa
     1681 gtcaaagcgt gtccgtccat tcatgaacaa acgaagagga gggggtacag gtggctgatc
     1741 aagaaaataa tacaaaagga caaatctggg cgtttatcgt cacaggcatt gtcatcctta
     1801 tcagtctcgc ctactacaag gcgaatgtat actatatgta tctcgtggta tacatctggt
     1861 tcggttttgc ctacgggatg atgcttcagt acggaagatt ctgcatggca tcggcttcgc
     1921 gagacctttt tgcagcgggg gttccgagaa tggcggtggg aatcatggtg gcactcatgt
     1981 tcttcagcat tattcaggcc ttgctttctg caacggatat gagcacgttt catcctgctc
     2041 cctttgggat ccatacgttg atatcgggaa ccatattcgg tgtgggaatg gtccttgccg
     2101 gagggtgtgc gtcaggttct ctttacaaaa tcggtgaagg aaatggcacc tcctttcttt
     2161 ccattatggg tatctgcttc ggtcaggcga tattctcagt ggttccctgg tttaacagta
     2221 tcatcccaca atcatgggaa atctctgcaa aggccaaaga tctccctgta agcctgaatg
     2281 cgtcatttga caagtacctg gcgggctatg tatgggatca gcccaagatt cagctttctc
     2341 atacaaaggt gatatcagag acgctccccg gcgtttcaaa gtattttctt ggcgatgcgc
     2401 tgatcagcgc gataattccc gctcttctgc tgcttgtcct catctacgcc gtctatatca
     2461 ggaaaggctt tatcaagaaa agggcaaaac agaaaggagg aaaaactggc ctgacagaca
     2521 atcttgctgg catatggagc atgattactg cctctaagag gaccacactt atgggtgtat
     2581 tgatcggcat tgtttgcggg ctccatattt ttgtcatgaa gggcatgcag attaagtttg
     2641 gggtgagcaa ctttggcgag ctgctcacca ggatgggtca taccgctgac gtttctctta
     2701 atgggacagt cttcgatccc ggctactggt atatcactag tcaagaggct cagtttggcg
     2761 catgggtaat ggaaaaattg ggtatcaata tgcgtgacaa tatcttcttc ggcgtcaata
     2821 acggtatccc cgaactctgg cgcaatcctg cgctttggat gtccataggt attatccttg
     2881 gcgccatggt catgtctctg atgaacaaag aattcaagtt aaagttgccg aaaggagagc
     2941 tcatcgtatg ggggctcctt ggaggaacgc tcatgggaat aggctccagg cctgcccttg
     3001 gctgcaatat aggtgcgttt ttcataaggg ttgcgggcgg tgaccccagc gggtggttat
     3061 ttgggattgg tatggcggta ggggcctttt ttggagtgaa gttctttaat tggtggtcag
     3121 agaggaagat ggccaaggaa atggcggatt tttagcaggc cagaacgcgt tgaagagata
     3181 aaagaataag gaggtaatta atcatggcaa tgaaatttga aaaaactgga gaaaaaacct
     3241 atttgcttga tgtgtgcggg tacgtatgtc cgcaccctca aatctactgc aagaagtcgc
     3301 tggagaagat tggcgaaggc gatgtgctcg agatggtatt tgacaatccc tcgtcagcag
     3361 agaccattat ccagatgctg gatcagggtg gacacgaggt cctcgagaag aagcacgaag
     3421 gcggcaagat aattttcaga attcaaaagg gataggaata tcagccctta cacaacgagg
     3481 agggtaggat gaaggcacct atggtgattt tgatactgat cgttgcggca gccctcggat
     3541 tcctgctggt tttcagccaa cagaaggaga agtctcccca ggctgcacaa ggacaggggg
     3601 cagcaggtta tggctcggca gcttcaggcg gaggcactgt ggcgcctcct tctggcgcgg
     3661 atggtaaatg aacggggcat gctaaccgag aagaaacggg tgggaaaatg aaaagattac
     3721 tcattgcagt ggacgatacg aagggcagta aagaggcatt caaattttgt accgagttct
     3781 gtaaatgcat gcttccggag aagattgtgc ttctctatgt ggaaaggttc gaggggagat
     3841 cctttatgac cgaaatgctt ccggactctg aacttggcac attaaaggag gtgctgcaag
     3901 ggactgagta taaggaggcg ctggacaaaa gggctgatga gatccttaat ttttacaagg
     3961 acacgcttgg ggacaatggt gtgaccaatg tgaagaccgt caagaaaata gggaatcctg
     4021 ctgaggaaat ccttaaaact gcaaaagaag aagatgtgga aatgattgtt atcggatcga
     4081 ggggcaaaag ggtgggccat ctccttatgg gaagcgtgag cagggaagta gccaacagct
     4141 cggatattcc cgtattggtt gttaaataaa tcaagaagat acaccagtca ttgattgccc
     4201 tgcgggtatc cgtagggcat tttttttgag gcatcttcgg agccttcggg cagagcaata
     4261 ggttataata aataaacatg gatttattct gtttaggatg gcggtaagaa tggatgagca
     4321 aggaacgaaa gtagtggtac tgggaggagg tctcacaggg ctctccgcag gttacctcct
     4381 gtcaaaggcc ggaaacaggg tggaggtatt tgagcgtgat tccatagtcg gtggtctctc
     4441 aaaaactatg gtccaggatg ggtttcggtt tgatctggga ggacacaggt tttttacaaa
     4501 agacaaaaaa attgatgact ttgtgaagac cctcatggaa ggggaactgg tctcagtatc
     4561 aaggaagagc aagatatata tgcgcaatcg atatttcgac tatcccctga agcccctcaa
     4621 tgccctcttt ggttttggcc tccccacaac aatgaaaatc cttggtgact acgggctcga
     4681 aaagtttaag ggaataatga gggaagagag aagcatctct cttgaggact gggtggtaca
     4741 gaattttgga cgaaaaatgt ttgacatcta ctttaaggaa tacagcgaga aggtatgggg
     4801 cattgaatgc agcaggataa gcgccgaatg ggtggaaacg cgtatcagcg ggctttccat
     4861 ggccaaggcg atcaaaaacg cgctgttcag cctcaatgga aaagatctcc caacccttgt
     4921 ggacaggttt ctctatcccc ggttgggaat aggacgcatt tccgagagac tcagggagga
     4981 gattgagagg aataataatg tccacaccga agcggacgtg cagcatattc atcacgccgg
     5041 cttcagcgtt aagagcattg cggtgaaaac caagaatcat ttggatctta tacagggaga
     5101 ggagtttatt tcgagcattc ccattacaaa actggtagcc atgctccgtc cgtcgcctcc
     5161 tgagtatatt ctggaggctg cttcaagact cagattcaga gaccttgtgg tggttgcgat
     5221 aatgataaac agggcgaggg ttactgacca gacctggata tatatcccgg aacagaagat
     5281 accttttgga aggatccacg agcccacaaa ctggagtgaa aagatggctc ccgcggggaa
     5341 gactattctg gtaacggagt tcttcagctt cgcaggtgat accctgtgga gcgagagcga
     5401 tgagaaactt gcggaag
//
LOCUS       DATKOU010000016         5221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_17931, whole genome shotgun sequence.
ACCESSION   DATKOU010000016 DATKOU010000000
VERSION     DATKOU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17931"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..236
                     /locus_tag="VMU21_00550"
     CDS             27..236
                     /locus_tag="VMU21_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HUO76040.1"
                     /translation="MKREEFMKHLRRYGCYIKREGSSHTIVQNIKNGASETVPRHNEI
                     DNVLVKKICGLTLRDPLKGKGARKV"
     gene            653..949
                     /locus_tag="VMU21_00555"
     CDS             653..949
                     /locus_tag="VMU21_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76041.1"
                     /translation="MRQLILLLSLSAIGTIFYALLKVISLNKEIPGGIVKESWRLLYY
                     LIGLLGVGYLTMPILPGLPETSREFIIAIIFLAGAIFILKVINLFDKIMRDVGL"
     gene            957..1541
                     /locus_tag="VMU21_00560"
     CDS             957..1541
                     /locus_tag="VMU21_00560"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76042.1"
                     /translation="MAIRDDLKNIIRAHMSDTVSSLFLDKSLSIIDESADNKESFISA
                     AERISKRTALFINADTAEGLFADLKARIGESASPQGKRRKYRRFPLYRKVHVICNGKS
                     RELYSEDISLGGIYIKTKEPFPEGSEVALTLHLEEGAQVQLHGMIVCTINESDASVKK
                     PAGIAIEFSTRGNVGIEKLRSYLEKISDQDVFED"
     gene            1659..2273
                     /gene="hisH"
                     /locus_tag="VMU21_00565"
     CDS             1659..2273
                     /gene="hisH"
                     /locus_tag="VMU21_00565"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HUO76043.1"
                     /translation="MIVIVDYGMGNLRSVEKGFLKVGVDARIVSDPKSVDDSSGIVLP
                     GVGAFRDCVKNLTDANLTDAIIRAVQKGKPFLGICLGLQVLFSESEEFGICRGLDVFR
                     GRVKKFPEMGLKVPHMGWNSVKILRRPPIFDNVADESYFYFVHSFYVAPQDRGIVATT
                     TEYGEEFTSMVWKDNIFATQFHPEKSQETGLRILKGFGEFVRKN"
     gene            complement(2382..2843)
                     /gene="folK"
                     /locus_tag="VMU21_00570"
     CDS             complement(2382..2843)
                     /gene="folK"
                     /locus_tag="VMU21_00570"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HUO76044.1"
                     /translation="MPIVHIGIGSNLGDREEHCLKAVRLFSEKGIHVRRRSSMYETEP
                     WGIKDQPRFVNMVIEGETGLTPARLLEVLKMTEDEVGRVETYRWGPRMIDLDILLYDD
                     LVMNTPELTIPHPRMHERDFVLRPLAEIAPDKIHPVLKKTVRELLSDLSTQ"
     gene            complement(2972..4147)
                     /locus_tag="VMU21_00575"
     CDS             complement(2972..4147)
                     /locus_tag="VMU21_00575"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HUO76045.1"
                     /translation="MKSRTFVELAERVRNLPPYLFAAIDRMKQEALAKGIDLIDLSIG
                     DPDIPTPEHIVDTMKRAVEKPGNHRYPSYEGMLTYREAVADWYRKRFRVALDPGNEVL
                     SLIGSKEGIGHIPLAFVNPGEVVLVPSPGYPVYPVGTLFAGGESHFMPLLEKNKFLPD
                     FSLIPGNVLKRAKLLFINYPNNPTSAVAPKRFYKDVVEFARKHRIIVCHDAAYSEIYY
                     DRKKPVSFLEIDGAKEVGIEFHSLSKTYNMTGWRIGFAAGNSEVVAGLGKIKTNLDSG
                     VFQAIQEAAITALRTDDADISKIRDIYQERRDALYHGLKSLGLHAIKPQATFYLWTKV
                     PSGFDSSGFVAHLLEEASVLATPGNGFGGPGEGYVRFSLTVPASRIREAVERIGKTL"
     gene            complement(4269..4724)
                     /locus_tag="VMU21_00580"
     CDS             complement(4269..4724)
                     /locus_tag="VMU21_00580"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HUO76046.1"
                     /translation="MKKAIAIIVSVLFVLSVAGLSFAAEKAAPAPAEKAEKKAPAKVK
                     QVTGEVAAVDAKANTLTVKGKKGDVMVNTDDKTSVKIGKEKKSLADVKMGDKVTVKYA
                     EMDGKNVAKSIMVSAAPAKEKKEMKKEAAPAAAPAAPAAAPAKKKTGGY"
     gene            complement(4889..>5221)
                     /locus_tag="VMU21_00585"
     CDS             complement(4889..>5221)
                     /locus_tag="VMU21_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUO76047.1"
                     /translation="VEYHWPGNVRQVESVIERAVLMCESDTIRLQDIQSELRLSRHQS
                     LFDIDVPEEGIVFEELEKELLKKAMEKANNVAARAARLLGMSYKTFWYRWEKFGLDGP
                     SSKRENIP"
BASE COUNT         1307 a         1261 c         1304 g         1349 t
ORIGIN      
        1 gaagcaattg aagaagaaat acttatatga aaagagaaga gtttatgaaa caccttcgga
       61 gatacgggtg ttacataaag agggaaggga gttctcatac cattgttcag aatatcaaga
      121 atggtgcctc agaaacagtt ccaagacaca acgaaataga taatgttctc gtaaagaaga
      181 tctgtggctt gacattaagg gaccctttga agggtaaagg ggcaagaaaa gtataaatag
      241 ggacagcgag gtataaaggc acataaggat acggggcgga aggcccgctc cctttttttg
      301 tgttcccaat tgactgcacc taagtatgtt ggactgtccg gaaatcatac agactgccgg
      361 aactacgcag aaaggcgttt cataccccac atgaccaacc ttgaaaaaag agattcaaaa
      421 tagcccttcc tgatgtgcgc ccatgaaata aacgaccaat aacatcctgt tttaataggt
      481 tatatttctg agttgttggg ctagactatt cgagctgcct ttcacggcct tcatgcaaga
      541 caggctggag aagaccgtga ctatcgcgtt gataagtatg gcaaaaaaat tgcatacagt
      601 cattaaaaga ctctatttgc gtattcgttt tgtaacgaag aaaggaggcg ttgtgaggca
      661 gctcatcttg ctcctgtcgc tttcggccat agggaccatc ttttatgcgc tcctgaaggt
      721 gatcagtctt aacaaggaga tacccggggg gattgtaaag gaatcctgga ggcttctcta
      781 ctaccttatc ggtcttctgg gggtggggta tctcaccatg ccgatactgc ccggcttgcc
      841 ggagacatca agggaattca ttatcgccat cattttcctt gccggggcaa tctttatcct
      901 gaaggtcatc aatcttttcg ataagataat gagggatgta ggattataga ggaaaaatgg
      961 ctatcaggga cgatctgaaa aacattatcc gcgcccatat gagcgacaca gtgagttctc
     1021 ttttccttga taagtcgttg tcgatcatag atgaatctgc ggacaataag gagagtttca
     1081 taagtgctgc ggagaggatc agcaaaagga ccgcgttgtt cattaacgct gacactgcag
     1141 aaggcttatt tgcagacctt aaggcaagga ttggagagag cgcttctcca cagggaaaac
     1201 gaaggaaata cagaaggttc cccttataca ggaaggtgca cgtaatctgc aacgggaaat
     1261 cccgggagct ttactctgaa gacatttcac tgggaggaat ctatatcaag acaaaggagc
     1321 cctttcccga gggttcggaa gtggcactca cactgcattt ggaagaggga gcccaggttc
     1381 agttgcatgg gatgatagtc tgcacgatca atgagtcaga tgcctcggtg aagaagcctg
     1441 ccggtatcgc aattgagttc agcacacgcg gtaatgtggg gatcgagaaa ctgaggagct
     1501 atctcgaaaa gatatcggac caggatgtct ttgaggatta ggataaagca ctgaagattc
     1561 ccagcgaact gcctctcagg ccaccctccg ctcttatgat ataatacgtt ggtgatcaga
     1621 attggtattg tggtaccgat ttttcagagg aggactgcgt gatcgtcatt gttgattacg
     1681 gcatggggaa cctcaggagc gtcgaaaagg ggttcctgaa ggtgggggtg gatgcgagga
     1741 tcgtgtctga cccgaaatcc gttgacgact cctcagggat cgtgcttccc ggcgtgggcg
     1801 ccttcaggga ctgcgtgaaa aacctcactg acgcaaacct cactgatgcc atcatcaggg
     1861 ctgtgcagaa agggaagcct tttctcggca tctgcctcgg tctccaggtg ctcttcagcg
     1921 agtccgagga attcggcatc tgcaggggtc ttgatgtctt cagggggagg gtgaagaagt
     1981 tccctgaaat ggggctgaag gtcccgcaca tgggctggaa cagcgtgaaa attttaagga
     2041 gaccccccat ctttgacaac gtggctgatg agagctattt ttattttgtg cactctttct
     2101 atgttgcacc ccaggacagg ggcattgtcg cgaccaccac ggaatacggc gaagagttca
     2161 cctcgatggt ctggaaggac aacatatttg ccacccagtt ccacccggaa aaaagccagg
     2221 agaccggtct gaggatattg aagggcttcg gcgagttcgt gaggaagaac tagccctggt
     2281 aattcataaa ttcggcagtt attcccgcgt gttctggaac ctcctgcagt tcaacagagc
     2341 tgcagtcctt gctaacacac aatggcggaa ttgcccatca tttactgtgt ggagagatcg
     2401 gaaagcaatt ctcgtaccgt cttttttaag accggatgga tcttatccgg ggctatttct
     2461 gccagtggtc gtaagacgaa atccctctcg tgcatacggg gatgggggat cgtgagttca
     2521 ggagtgttca tgacaaggtc atcataaagc agtatatcca ggtcaatcat ccggggaccc
     2581 cagcggtagg tctccaccct tccgacctca tcctcggtca ttttcagaac ctcaagaagc
     2641 ctcgcgggcg tcaacccggt ctctccctcg atgaccatat tgacaaatct cggctggtct
     2701 tttatccccc acggctctgt ctcatacatt gacgagcgcc tcctgacatg aatccccttt
     2761 tcggagaaaa gccggaccgc cttcagacag tgttcttccc tgtcaccgag gttggagccg
     2821 attccgatat gaacgattgg catatgaatc caaatggttc tcgtgaaacg cagaatcatc
     2881 ggagagaaat aatcaaaaaa agtattaagg ctaaggttga aaggttctcg ccctgatctt
     2941 cttaatctca acctcgacct ggtttctgtt attaaagggt cttccctatt ctctcgaccg
     3001 cctctctgat cctgctggca gggacggtga gggagaatct cacataaccc tctcccggcc
     3061 cgccgaatcc gtttccggga gtagcaagga cagacgcctc ttcgaggaga tgggccacaa
     3121 atcctgaaga gtcaaaccct gaaggaacct ttgtccagag atagaaagtc gcctggggtt
     3181 ttatggcgtg gagacctaga ctcttcagtc catggtaaag ggcatctctt ctttcctggt
     3241 agatatccct tatctttgag atatctgcat catctgtcct gagggcggtg atcgcagcct
     3301 cctgaattgc ctggaacacc cctgaatcca gattggtctt tatctttccc aggccggcaa
     3361 cgacctcact gttgcccgcc gcaaatccga ttctccatcc cgtcatattg taggtcttgg
     3421 aaagggagtg gaattcaatg cccacttcct ttgcaccatc aatttccaga aagctcacgg
     3481 gctttttgcg gtcgtaataa atctccgagt aggctgcatc atggcagacg ataatacggt
     3541 gtttccgcgc gaattccacc acgtccttat aaaaccgctt tggcgcaacg gctgaagtgg
     3601 ggttgttcgg gtaatttatg aagagtaact ttgccctctt gagcacattt ccgggtatga
     3661 gtgagaaatc tggcaggaat ttgttcttct ccaaaagcgg catgaaatgg ctttcgcccc
     3721 cggcaaagag tgttcccacc ggatacacag ggtatccggg cgaggggacc agcaccacct
     3781 ctccgggatt tacgaaggcc agggggatat gccctatgcc ctcttttgag ccgatgaggg
     3841 acagtacctc gttccctggg tcgagggcca ccctgaatct cttcctgtac caatctgcaa
     3901 ctgcctccct gtaggtgagc atgccctcat aggacgggta gcggtggttg cccggctttt
     3961 ctactgcccg cttcatggta tccacaatgt gttctggtgt gggaatgtca ggatcaccga
     4021 tactgagatc aattaaatca attccctttg caagcgcctc ctgcttcatt ctgtctattg
     4081 ctgcaaagag atagggcggc aggttcctta ccctttcagc gagttccaca aaagtcctgg
     4141 atttcaaatc ttcctcccga tgattttatg gattgtatta taaacgaaaa ggtcactaca
     4201 aaaaataagg ttaaaaaaaa gggcactgta ttcagtgccc tttttctctt caactggcta
     4261 accagtgatt aatagcctcc ggtctttttc tttgcaggag cagcagcagg ggctgcaggg
     4321 gcggcggcag gagctgcctc cttcttcatc tctttcttct cttttgcagg agctgcgctc
     4381 accatgatgc tcttcgcaac gttcttgccg tccatctcgg cgtacttcac agtcacttta
     4441 tcgcccatct tcacgtccgc aagggacttc ttctctttgc cgatctttac gctggtcttg
     4501 tcgtctgtgt tgaccatgac atcacccttc ttacccttta cggtgagggt gtttgccttt
     4561 gcgtcaactg ctgcgacctc tccggtaacc tgctttacct ttgcaggggc cttcttctca
     4621 gccttctctg caggagcagg agctgccttc tcagctgcga aggagagacc ggcgactgaa
     4681 aggacaaaga gaactgatac gataatcgct attgcctttt tcataaaatc acctcctttc
     4741 attatgagct aatcaaaacc tgataggatt aatacaagat gcgtgccagt tcaaggagaa
     4801 tagacagggg gtaaaataat attataaatt agtaagttat gtaatgggcc attttaaagg
     4861 ggagaagttt tcaaaaagat ttcccttttt aaggaatatt ctcccttttt gaggaaggac
     4921 cgtccagtcc gaatttttcc caacggtacc agaaggtctt gtaactcatg ccgaggagcc
     4981 gcgctgccct ggcagccacg ttgttcgcct tctccatagc tttcttgagg agttcctttt
     5041 ccagttcctc aaagacaatg ccctcttcag gcacgtcaat atcgaaaaga ctttggtgac
     5101 gggataagcg caactcgctt tgtatgtcct gcagccgtat tgtgtccgac tcgcacatga
     5161 gcacagcccg ctcaatgaca gactctactt gcctcacatt gccgggccag tggtattcca
     5221 c
//
LOCUS       DATKOU010000017         3812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3869934, whole genome shotgun sequence.
ACCESSION   DATKOU010000017 DATKOU010000000
VERSION     DATKOU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869934"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /gene="rpoN"
                     /locus_tag="VMU21_00590"
     CDS             complement(<1..1397)
                     /gene="rpoN"
                     /locus_tag="VMU21_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HUO76048.1"
                     /translation="MALESRLELKLTQKLILTPQLQLAIKLLQMPQLELSQALTQELT
                     ENPFLEEIIEEREELSREEMESMERAEEPDDTEAPLEKLIAGLGSYGVDDYFDERSSD
                     GRDLGYFTPGTVEHPSFEQFVKEEADLSDHLLWQLRLSDADEETKQIAEVIIGNIDEN
                     GYLRATEEEIVQGAKASAEKVQSALSLVQGFDPPGIAARNLQECLLIQLYSLGLRGSL
                     AEKIILHNMSDIEKKRYPQIARQYQVSLDDVMAAVKVIEAMEPKPARNFSTLAPTYIV
                     PDVTVIKTEDGYQIILNDEGLPRLRLNSFYRKLFHSKNALSKEEKLFIEEKLRAAVWL
                     LKSLDQRNRTIYRVTESILNFQGDFFNKGVSYLKPLNLRDVALSLGMHESTISRVTSN
                     KYLSCGHGLFSFRFFFSSALQSAEGAISSTSVKDLIRKIILEEDTKKPLSDQRIVELL
                     KSKDITIARRTVAKYR"
     gene            complement(1397..2122)
                     /gene="lptB"
                     /locus_tag="VMU21_00595"
     CDS             complement(1397..2122)
                     /gene="lptB"
                     /locus_tag="VMU21_00595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HUO76049.1"
                     /translation="MVHNLRANGLSKIYRGKKAVDDLSLYLTTGEIVGLLGPNGAGKT
                     TTFYMLVGMIRPDRGSITLDDEDIGQLPMYKRALKGISYLPQEASIFRKLSVRDNLRA
                     ILEIKGYSREEMGDKVESLLDEFGLSGFADRHSYRLSGGERRRTEIARAIATNPTFIL
                     FDEPFAGIDPLAIIELKKMLQYLKEKGLGILLTDHNVRDTLSITDRAYIITNGGILDE
                     GIPDALIANPRVKETYLGEEFRL"
     gene            complement(2143..2649)
                     /locus_tag="VMU21_00600"
     CDS             complement(2143..2649)
                     /locus_tag="VMU21_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HUO76050.1"
                     /translation="MSKPPFFETLKVTCFIGLLSCLVLFSLCFRSSGEEKLIEKKGPI
                     TITSSTMTADNKAHTALFEGSVMAKTETTIIYADKMLVYYTDAGKVTRIEASGHVKLL
                     KGERVITSDEATFLADEEKVIFTGQPKAVEGTTMVTGTKMIYLMKEDRSLVEQSRVFM
                     EKGTPKTQ"
     gene            complement(2627..3127)
                     /gene="lptC"
                     /locus_tag="VMU21_00605"
     CDS             complement(2627..3127)
                     /gene="lptC"
                     /locus_tag="VMU21_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HUO76051.1"
                     /translation="MLGGILFIFTCLVFYSVFSGEHDIKKDLSIQRGNSYIEGLKIVN
                     KKDGADTSVVTARRADFTRDETVAQMETVAIDLKKEGVVVSADNGTYHMQTRDLRLGN
                     NIKIQIKGSVLTTDELSWNPSRGMLFSKDVVRMEGNKFRIEGEGLTATQDNKVKLMRN
                     VKATFF"
     gene            complement(3503..>3812)
                     /locus_tag="VMU21_00610"
     CDS             complement(3503..>3812)
                     /locus_tag="VMU21_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76052.1"
                     /translation="NVTSLKKLDTLSGVDVLINATPLGLKDTDPSPLNISLIPRTITV
                     CDLIYRDTALLRAASERGCRTLNGLGMLLHQGVLAFEIWTGVRPPVEIMRNALKAALQ
                     "
BASE COUNT          877 a          970 c          932 g         1033 t
ORIGIN      
        1 cggtatttgg caactgtcct ccttgcaata gtgatatctt ttgacttgag aagttcgacg
       61 atcctctggt cgctcaatgg tttcttggtg tcctcttcaa ggataatctt tctgatgagg
      121 tcttttactg aggtggaaga gatggcaccc tcagcgctct gaagtgcgct gctgaagaaa
      181 aacctgaaac tgaaaagacc gtgaccgcac gagaggtatt tgttcgaggt gaccctgctt
      241 atggtgcttt catgcatccc gaggctcagg gcaacatccc tgagattgag gggcttcagg
      301 taggaaactc ccttattaaa gaaatcaccc tggaagttca gtatgctctc ggtgacgcgg
      361 tagatggtcc tgttcctctg gtccagactc ttaagaagcc agaccgccgc ccgaagcttc
      421 tcctcaatga agagcttttc ttccttagag agggcgttct ttgaatggaa aagctttcgg
      481 tagaagctgt tgagcctgag tcgcggcaga ccttcgtcat tgagaataat ctgatatccg
      541 tcttcggtct tgatcactgt tacatcggga acgatatagg tcggggcgag ggtgctaaag
      601 ttcctcgcag gctttggttc catggcctcg ataaccttga cagcggccat tacatcgtca
      661 agagagacct gatactgtct tgcaatctgt gggtagcgtt ttttttctat atcactcata
      721 ttgtggagga taatcttctc tgcaaggctc cccctgaggc ccaatgaata cagctggatg
      781 agaagacact cctggagatt cctcgccgca atcccgggcg gatcaaatcc ctggacaagg
      841 ctgagggcag attgcacctt ctctgcgctg gccttggcac cctggactat ctcttcttcg
      901 gtggccctaa gatatccgtt ttcatcgata ttaccgataa tgacctctgc gatctgtttt
      961 gtctcttcgt cggcatctga gagcctgagc tgccagagga gatggtcgga aaggtctgct
     1021 tcttccttta caaactgttc aaaggacgga tgctccacag taccgggcgt aaagtatccc
     1081 aggtcccgcc cgtcgctgct ccgctcatca aaatagtcgt ccacgccgta gctgccaaga
     1141 cctgcgatga gcttctcaag aggggcttcc gtatcgtcag gctcctcagc cctttccatg
     1201 ctctccatct cctccctgct cagctcttcc ctctcctcaa ttatctcttc gagaaaagga
     1261 ttttcggtga gttcctgggt aagtgcctgg gacaattcaa gctggggcat ctggagaagt
     1321 tttatggcga gttggagctg gggagtgaga ataagctttt gtgtaagttt gagttctaat
     1381 ctgctttcca gtgccatcag agtctgaatt cctcccccag atacgtttcc tttacccttg
     1441 ggttggcaat gagcgcatcc ggaatccctt cgtccagtat gcctccattg gttatgatat
     1501 aggccctatc ggtaatggag agggtgtccc tcacattgtg atcagtgagc agaattccca
     1561 accccttttc cttgaggtac tgcagcatct ttttcaattc aatgatggcg agggggtcga
     1621 tccccgcaaa gggttcatca aaaaggataa aggtgggatt tgtggcgatg gcccttgcaa
     1681 tctctgtcct tcgcctctcg ccccctgaaa gtctgtagga gtgcctgtct gcaaagcccg
     1741 agaggccgaa ttcatccagg aggctttcca ccttgtcccc catctcctca cgagagtacc
     1801 ccttgatctc aaggattgct ctgagattat cccgcaccga gagcttcctg aaaatggatg
     1861 cttcctgcgg cagatagctg atacccttga gtgccctctt gtacatgggg agttgaccaa
     1921 tgtcctcatc gtcgagagtg atgcttcccc tgtccggtct gatcattccc acgagcatat
     1981 agaaggttgt ggttttacct gcgccattgg ggccgagaag ccccacgatt tcccctgtgg
     2041 taagataaag gctgaggtcg tcaaccgcct tttttccacg gtagattttt gaaaggccat
     2101 ttgcccgaag attatgtacc attccctatc agtcctgcag tcctattggg ttttaggagt
     2161 ccctttttcc ataaataccc tgctttgttc caccagtgaa cggtcttcct tcatcagata
     2221 aatcattttt gtgccggtca ccatggtagt tccctccaca gccttaggtt gaccggtgaa
     2281 aattaccttt tcttcatccg caagaaaagt ggcctcatca gaggtaatca cgcgctcgcc
     2341 ctttaggagc tttacatggc cgctggcctc aattcttgtc acctttcccg catcggtgta
     2401 gtagacgagc atcttgtcgg catatatgat cgtggtctcg gtttttgcca tcacagagcc
     2461 ctcgaaaagg gcagtatgag ctttgttatc cgcagtcatt gtggaagagg ttatggttat
     2521 ggggcctttt ttctctatga gcttctcctc ccctgaggac cggaaacaaa gggagaagag
     2581 aacgagacat gaaagaaggc cgatgaaaca cgtgaccttc aaggtttcaa aaaaaggtgg
     2641 ctttgacatt tctcatcagc ttgaccttgt tgtcttgggt ggcggtcagc ccttctccct
     2701 ctattctgaa cttgttcccc tccattcgga ccacatcctt ggaaaatagc attccgcgcg
     2761 agggattcca tgaaagctca tccgtagtga gcacggagcc ctttatctgt atcttgatat
     2821 tgttccctaa acggaggtcc cgggtctgca tgtgataggt accgttgtcg gcactgacta
     2881 ctaccccctc cttcttgagg tcaatggcca cggtctccat ctgtgccacg gtctcgtccc
     2941 tggtaaagtc agccctgcga gcagttacaa ccgatgtatc cgcaccgtcc ttcttgttca
     3001 cgattttcag gccttcaata taggaatttc cgcgctgaat ggaaaggtct ttcttgatgt
     3061 catgttctcc actgaagact gagtagaata ccaaacacgt gaagatgaac aggatgccgc
     3121 cgagcatcct ccggcctctg aaaaaaggtg gaatcagttc ataaaattcc tgcatcaatt
     3181 aaatcttatc acattgaaat accttagtaa agcaaaaaat ataccacaat tctgatttaa
     3241 cctaaaaaaa tatgtagacg cctaagcacc ggtaaagatg cttttaaaac gaacagcgag
     3301 cgcattccct gtccgcctca ggcggactgc tggcttaagg agcgaatatg ataaagaaca
     3361 attccttacg ttaagaaccc tgcggtcttg ccgcagggtt cttcatatgt ccaggcctct
     3421 ccctaaccct acgcaacgaa agttgacttg gacagatagg ggtaccagtt cttgccatgc
     3481 acgggagagg aggcaaaaaa tgctattgaa gggcggcctt aagggcgtta cgcattatct
     3541 ccacaggggg tcttactcct gtccatatct caaaggcgag aacaccctgg tggaggagca
     3601 tccctagacc gttgagggtt ctgcagcccc tttctgaggc agccctaagg agagcggtat
     3661 ccctgtagat aagatcgcag acagtgattg tcctgggaat gagcgaaatg ttgagggggg
     3721 aagggtcagt atccttcaga ccgaggggag tggcattaat cagaacatca acaccggaga
     3781 gcgtatccaa ttttttcagc gaggttacat tg
//
LOCUS       DATKOU010000018         7855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2331382, whole genome shotgun sequence.
ACCESSION   DATKOU010000018 DATKOU010000000
VERSION     DATKOU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331382"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..405)
                     /locus_tag="VMU21_00615"
     CDS             complement(139..405)
                     /locus_tag="VMU21_00615"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HUO76053.1"
                     /translation="MGSVEVYILGQKYTIKGDAPEEYIQQLANYVQDKIKEVYNNSPS
                     ITPVKASILAAITIADELHRLRNEQENLTKSIEEKTVELTRLFD"
     gene            complement(409..699)
                     /locus_tag="VMU21_00620"
     CDS             complement(409..699)
                     /locus_tag="VMU21_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76054.1"
                     /translation="MGKTLFDDDTFSKSLNSKKEEKPLNRLKNLDEKIADAVNRVKAL
                     KEEKVVLERRIRDLEAQLTEKNREVERLSSEKTAIKNQVEDLLNELETLEIG"
     gene            complement(716..2752)
                     /locus_tag="VMU21_00625"
     CDS             complement(716..2752)
                     /locus_tag="VMU21_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUO76055.1"
                     /translation="MKKLFTLIFILVIFPLAYADELDGKRPLQVSVASVDSLTNLKRG
                     KELFDKAEYTEALERFKSAYEEIPVIRDFTLLMMAKAYNRTARFGESGDALRELLGTY
                     PDSLFMQRARVLQINSLMHVAEITPREKRFSEKSAAALKHMESYVTDYPEDVEMTFFL
                     AQLMKKLGKVQNAKKLFIGIYTGVSPYSESAFQELQPADITLDDILVKASNLIKAFEH
                     KKAESLIRKALQKADASQEDELYRKLGLALFKQKKYREAADAFLKGGDLYDGARSLYR
                     AENFTAFNEILSQLISMEDTRAGALLLAYASTRRREGKKDETLNIYAGLRKNFPQYGE
                     EALWGVAWTYYRAGNYTEASKILTELNDRYPNPRYRYWKQESNRGNSSDTAFSEKQKQ
                     DPISQKDFYSLLTLMRDPDQFSGRSIRRIAYTPTPDQTVPMSRQASIEFEELTPEIRS
                     SLERFRILVELEMREDAISELVRISNRVSRPDALVYLCHILQNAGDYKRAISLVSRFT
                     EHAPQGNGQMNIYDILYPIAYWPTVREISQHNMLDPLLLLSVMREESRFDPRARSVAG
                     ALGLMQIMPHTARNLDRRLNLDISDDAAIYDVRTNITIGGYYLNSLLKEFGSLPVALA
                     AYNAGHDTVREWIKKGNYRSFDEFIEDIPYDETRNYVKRVLLTYATYMNLWDKQ"
     gene            2884..3645
                     /gene="fdhD"
                     /locus_tag="VMU21_00630"
     CDS             2884..3645
                     /gene="fdhD"
                     /locus_tag="VMU21_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HUO76056.1"
                     /translation="MDKVAKRSICRHTDGSTEYMEDLIALEKRVRISVNGKEVMGLYC
                     TPHMVKELVVGLIMTEGIIRGNWCVERMSIVYGDEISVDIPAEGEVVTEGKTITSGCV
                     GGVTFDRKAEEDMITDTFVLHRADLKYIFNDFQARSELYKTTGCVHSAALSDGRIIIC
                     FAEDIGRHNAVDKVIGYALLEHIPFSGKILLASGRISSEISSKCSRWGIPILATRTAP
                     TNLAVEIAEKRGVTIVGFVRGNRFNIYTHPKRIVL"
     gene            complement(3663..5027)
                     /locus_tag="VMU21_00635"
     CDS             complement(3663..5027)
                     /locus_tag="VMU21_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO76057.1"
                     /translation="MEEHKGKILVIEDEKNMREILKILLEGEGYKVSTATDGIEGIGW
                     INREIFDLIITDIKMPGLDGFQVLKKAQELSPETLVIMITAFGTTESAIESMKLGAYD
                     YLHKPFKIDEIRLVVKNALEKRKLRGEVSILRDKIRTTYELGNIFYKSPKMQELLRLL
                     PKIANSNSNVLITGESGTGKEFATTAIHNLSPRRDRNLVAINCASFPEGLLESELFGH
                     MRGSFTGAVYNKQGLFEIANGGTLFLDEIVEMPVNLQAKLLRVLQNGTFRRVGGTTDV
                     TVDVRIVAATNRNPVEEVSAGRFREDLYYRLNVVPLHIPPLRERTEDIPLLVDHFIKK
                     FSSTPRRLSPEAMKLLMHHPWKGNVRELENIIERVLLLTDKEEITPPDIPLEISDTGW
                     ESNVLPKMEKPGIDLDAIVEGIEKRYLLEALQFTNGAKTEAAKMLNLSFRSFRHRLQK
                     YGIK"
     gene            complement(5056..6717)
                     /locus_tag="VMU21_00640"
     CDS             complement(5056..6717)
                     /locus_tag="VMU21_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO76058.1"
                     /translation="MPDNALFKRLKALIVFRAFFVTFLLGTFLFFGIGFYKFPYTHTI
                     LYFIIIPLYILSVVYGLFLPRIRNLVIFAHLQLFADAISSVSLIFLTGGIESWFSFIM
                     LLIVIGSVITVNRKAGYITATICSILYGLMIDLQFYHLIPVPYDPSVMEKDFLYNIFS
                     HISGLYLTAYLTGYLSSRLEKTSKKLEEKDSNLKDLTFFHKELIESLPSGLLTTDLSG
                     SILIFNRAAEHITGVLRTAAIGNNITTVFPFVIPPIETLRGEGMITVHGTRRTIGLTL
                     SQSTDSSGHETGYICIFQDITDLKRLEAELKHKETLAAIGELSANMAHEIRNPLASLK
                     GSIEILKEGTLTRAHGEKLMDIALSEMDRLNTIITDFLTYSRPKPPEFLSFDLHVMLD
                     EIIQLTRNAIMSPDNISITKDFGGHYEIVADPQKLRQVFLNLTMNAVESMPDGGSLII
                     KTRRVHDTVAVSFRDSGIGIPPENLKEIFYPFFTTKDRGTGLGLSIAYRIIEEHRGRI
                     TVNSIPGTGTTFEVTIPVSQPSSGSTPEMQNSRAKGEHAGLARPF"
     gene            complement(6725..>7855)
                     /locus_tag="VMU21_00645"
     CDS             complement(6725..>7855)
                     /locus_tag="VMU21_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUO76059.1"
                     /translation="AQLRRKNITATVVTEKTETKKGLLSGLSLGGGVSDKDIVVFTRQ
                     FSTMIDAGLPLVQGLEILSTQVENKSLAKNLTVIKADVEGGSTYADALRKHPKIFSEL
                     YVNMVAAGEAGGILDTILNRLAAYIEKAMKLKKKVKGAMIYPAVVTSVAVLVIAVIMI
                     FVIPTFSQMFASLGGTLPLPTRIVVGMSHFIAGIGGLIVAGLIVAIIVSIVQIRKTEK
                     GKKVIDSIALKLPIFGILLRKVAVAKFTRTLGTLISSGVPILDGLEITAKTAGNKVIE
                     YAIMDVRQAVSEGKTLEEPLTKAKVFPPMVTHMIAVGESTGALDAMLGKIADFYDDEV
                     DNAVANLTAMMEPLLMVFLGGAIGFIVVAMYLPIFKMITLIK"
BASE COUNT         1877 a         1822 c         1907 g         2249 t
ORIGIN      
        1 tccgcctttt cctgcaagtg caatgagata tgccattttc gattatacaa taaaaagcca
       61 agaatatgct cagggattag tggaatttcg taaaagaaaa gattctcttg atgttccctc
      121 gccgatttga agagtttctc agtcaaacaa tcgcgtcagc tcgaccgtct tttcttctat
      181 gctctttgtg agattttcct gttcgtttct gagcctgtgg agttcatccg cgatggttat
      241 ggctgcaaga attgatgcct tgaccggagt gatgctgggt gaattattat acacctcttt
      301 tatcttgtcc tggacatagt tggcaagttg ttgtatgtat tcctcaggtg catcaccctt
      361 aatggtatat ttctggccga gtatatacac ttctacagat cccatttctt acccaatctc
      421 cagcgtttca agttcgttca agagatcttc cacctggttt tttattgccg tcttttcaga
      481 agagagtctc tccacttccc ggtttttctc cgtgagttgt gcctccagat ccctgatccg
      541 ccgctctaaa actacctttt cctctttcag ggccttcacc ctgtttactg cgtctgcgat
      601 cttttcatca agatttttaa gcctgttcaa tggtttctcc tccttcttgc tattcaaact
      661 tttggaaaat gtatcgtcat caaagagtgt ttttcccatg gtctccccgg tttccctatt
      721 gtttatccca taggttcata taggttgcat aggtcaaaag cacacgttta acatagtttc
      781 tggtctcgtc atagggtata tcttcaataa attcgtcaaa ggatctgtag tttcctttct
      841 ttatccattc cctcaccgtg tcatgccctg cgttataggc ggcaagggca acaggcaaag
      901 acccgaattc ctttagcagg gaattaaggt agtatccgcc aatggttata ttggtcctta
      961 catcgtagat tgcggcatcg tcagagatgt ccagattgag gcgtctatcg aggttgcgtg
     1021 ctgtatgggg cattatctgc ataaggccaa gagccccggc aacggaacgg gcacggggat
     1081 cgaagcggct ctcttccctc atcacagaca agagaagcag aggatcgagc atattgtgtt
     1141 gagatatttc acggactgtt ggccaatatg caatgggata caggatatca taaatgttca
     1201 tttggccatt cccttgtggc gcatgttctg tgaatcttga tacgagggat atggcccgtt
     1261 tgtaatctcc cgcattttgt aagatatggc ataaatagac aagcgcatca ggccttgaga
     1321 cgcgatttga tatgcggaca agctctgaga tcgcatcctc tctcatctca agctccacga
     1381 gtatccggaa ccgctccaac gacgagcgga tctcaggggt aagctcctcg aattcgatgg
     1441 aagcctgtct gctcatgggc acagtctgat caggagtcgg tgtatatgcg atacgtctga
     1501 tggaacggcc gctgaactgg tcagggtctc tcatgagtgt gagcaggctg taaaaatctt
     1561 tttgggaaat tggatcttgt ttctgctttt cagaaaaagc cgtgtcggat gagtttcctc
     1621 ggttgctttc ctgtttccag taacggtatc ggggattagg ataccggtca ttgagctccg
     1681 ttaaaatctt ggatgcctcc gtataattcc cagcgcggta gtaagtccag gcaacccccc
     1741 acagcgcttc ctctccgtac tgcgggaagt ttttcctaag ccccgcataa atattcaggg
     1801 tctcgtcctt tttgccctct cttcttcttg ttgaggcgta cgcgagcaaa agggcacccg
     1861 cgcgtgtgtc ttccatggaa ataagttgag agaggatttc attgaacgcg gtgaagtttt
     1921 ctgccctgta aagggaacga gcgccgtcgt aaaggtctcc tcccttcagg aatgcatcag
     1981 cagcttccct gtattttttc tgcttgaaga gggcaaggcc cagctttctg taaagttcgt
     2041 cttcttgact ggcgtccgcc ttttgaagtg ctttccggat gaggctttcc gctttcttat
     2101 gctcgaatgc cttgatgagg tttgatgcct tcacaagaat atcatccagg gtgatatctg
     2161 cgggttgtag ttcctgaaac gcagactctg aatatgggct gacacctgta tatatcccga
     2221 tgaagagctt tttcgcgttt tggacctttc caagtttttt catcagctga gcaagaaaaa
     2281 atgtcatttc cacatcctca ggataatcgg tcacatagga ttccatgtgc tttagtgcgg
     2341 cagcgctctt ctccgagaat ctcttttccc tcggggttat ttctgcgacg tgcataagcg
     2401 aattgatctg taaaaccctt gccctctgca taaacagtga atcgggatag gtgcccaaga
     2461 gctcgcggag agcgtccccg gattccccga accttgcggt cctgttatat gcctttgcca
     2521 tcatgaggag cgtaaaatcc cttatgacag ggatttcctc ataggcactc ttgaagcgtt
     2581 ccagcgcctc tgtatattct gccttatcga agagttcctt gccccttttg aggttcgtaa
     2641 gggaatccac agaggccacg gatacctgaa gcggtctctt gccgtcaagt tcatctgcgt
     2701 aggcaagagg gaatattaca aggataaaga tgagtgtaaa gagttttttc ataatcaatt
     2761 cgcggagtac gcttatctat tatataaata atgatgggca gattcaactg aaaattggct
     2821 ttttacgtta tgaaatcctc atgtaagttt gacggaaagg ctgatgaaca gataaaatgc
     2881 actatggata aagttgcaaa gaggagcatt tgcagacata cggatggctc tacggagtat
     2941 atggaggacc ttattgccct tgagaaaagg gtgagaatat ctgtcaacgg aaaggaggtg
     3001 atgggtctct attgcactcc acatatggtg aaggaactcg tagtgggcct cataatgaca
     3061 gaggggatta taagggggaa ctggtgtgtg gagaggatga gtatcgtcta cggagatgaa
     3121 atatccgtgg atatcccggc agaaggggag gtagtgacag aaggaaaaac tattacctcg
     3181 ggatgtgtgg gcggtgtcac ttttgacagg aaagcggaag aagacatgat caccgacaca
     3241 ttcgtcttac atagggctga tctgaaatat atctttaacg actttcaggc caggtcagag
     3301 ctttacaaga ccacaggatg tgtgcacagc gctgcccttt cggatggaag gatcattatc
     3361 tgttttgcag aggacatcgg aaggcacaat gcggtagaca aggtgattgg ctatgccctg
     3421 ctcgaacaca tccccttttc agggaaaata ttacttgcaa gcgggcgaat ttcttccgag
     3481 atttcctcaa aatgttcccg atggggcatc cccatccttg ccacccgtac tgctcccaca
     3541 aaccttgcag tggagattgc cgagaaacgt ggggtaacca tcgtaggctt tgtgaggggg
     3601 aacaggttca atatatacac ccatcccaag cggatcgtgt tgtaattcag gactttcccg
     3661 ggttacttta tcccgtattt ctgaagcctg tgtctgaatg acctgaaaga gagattcagc
     3721 atctttgccg cctctgtctt tgccccgttg gtgaactgaa gggcttcgag gagatatctt
     3781 ttttctatcc cttccactat ggcatcaaga tcgatccctg gtttttccat tttgggaagc
     3841 acgttactct cccaaccggt atcactgatc tccagaggga tgtccggggg ggtaatttct
     3901 tccttgtcgg tcaggaggag gactctttct atgatatttt cgagttcccg cacattcccc
     3961 ttccatggat gatgcatgag gagtttcatg gcctcagggg ataaacggcg tggtgttgag
     4021 gagaacttct tgataaaatg gtcaacgagg agaggaatat cctccgtcct ttcccttaaa
     4081 gggggaatat ggagcggcac gacattcaga cggtaataga gatcctcgcg aaatctcccc
     4141 gcggagacct cttccacagg attcctgtta gttgccgcta caatcctcac gtctaccgtt
     4201 acgtcggttg ttcccccaac cctcctgaat gttccgttct ggagaaccct gaggagtttt
     4261 gcctggaggt tgacgggcat ctccactatt tcgtcaagaa aaagggtacc gccgttggca
     4321 atctcgaaaa gtccctgctt attgtatacc gcgccggtaa atgaacccct catatgcccg
     4381 aagagctcac tttccagaag cccctcggga aatgacgcgc agtttatcgc aacgagattt
     4441 cggtcccttc tgggactaag attgtggatc gcggtggtag cgaactcctt tcctgttcca
     4501 ctttctcctg tgataagaac attcgaattg ctgttggcga ttttcgggag gagtctgagt
     4561 aattcctgca tctttgggct cttgtagaaa atattgccaa gttcataggt agttcgtatt
     4621 ttgtcgcgaa ggatcgagac ctctccacgc aatttccgtt tctcaagggc gttcttcacc
     4681 actaaccgta tctcgtcgat cttgaagggt ttatggagat aatcgtatgc gcccagcttc
     4741 atggactcaa tagcggattc tgtggtgcca aaggcggtga tcatgatcac tagggtttca
     4801 ggtgacaact cttgagcctt cttgagaacc tgaaagccat caagccccgg catctttatg
     4861 tcggtaataa taagatcaaa gatctctcta tttatccatc cgataccttc tataccatcc
     4921 gttgcagtgg acaccttgta gccttctcct tccaggagta tctttaggat ttccctcatg
     4981 ttcttttcat cctcaatcac gagtatcttg cccttatgtt cttccatagg ttatatccat
     5041 ttgggtgtct ttatttcaga atggcctggc cagaccggcg tgctcccctt tggcacgaga
     5101 attctgcatt tcaggagtgc ttccagatga aggctggctc acggggattg ttacctcaaa
     5161 tgttgttcct gtccccggaa tactattgac cgtgattctg cctcgatgtt cttcgatgat
     5221 cctgtacgcg atggaaagac cgagaccagt tcccctatcc tttgtggtaa agaacggata
     5281 gaatatctcc ttcagatttt cgggagggat tccaattccg gaatctctaa aggagacggc
     5341 cactgtatca tgaaccctcc ttgtctttat gatgagggaa cctccgtcag gcattgattc
     5401 tacggcattc atggtgagat tcagaaaaac ctggcgcagt ttctggggat ctgcgactat
     5461 ctcatagtgg cccccgaaat cttttgtaat cgatatatta tccgggctca tgatcgcatt
     5521 ccttgtgagc tgtatgatct cgtcaagcat gacgtggaga tcaaagctca ggaattcagg
     5581 tggtttcggc ctcgagtaag tgagaaaatc ggtgatgatg gtatttaaac gatccatctc
     5641 gctcagggca atatccatta atttctctcc gtgagccctt gtcagtgttc cctctttgag
     5701 aatctcgatg gatcccttga gggaggcgag aggatttcta atttcatgag ccatatttgc
     5761 agagagttct cctatggcgg ctagcgtctc tttgtgcttt aattcagcct caagtctctt
     5821 caggtctgta atgtcttgga aaatgcagat atatcccgtt tcatgtccgc tgctgtctgt
     5881 agactgcgag agggtgagcc cgatagtcct gcgagtgccg tgaacggtaa tcatgccctc
     5941 tcccctcagt gtttcgatag ggggtatgac aaaagggaag acggtggtga tattattccc
     6001 gatagcagca gttcggagaa cgccggtgat gtgttccgct gcgcggttga agattaagat
     6061 actccctgaa aggtctgtgg tgagaagtcc gctgggaagg ctctctatca gttccttgtg
     6121 aaagaaggta aggtccttga gattagaatc cttttcttcc agcttcttcg acgtcttttc
     6181 taaccgggag gaaagatatc ccgtcaggta cgcggtgaga tataacccgc taatatggga
     6241 aaagatattg tagagaaagt ctttctccat cacggaggga tcatacggga cgggaatgag
     6301 atggtaaaac tgaagatcta tcatgaggcc gtaaagaatg ctgcaaatcg ttgcagtgat
     6361 atatcccgct tttctattca ctgtgattac tgagccgatg acgatcagga gcattatgaa
     6421 ggaaaaccag ctttcaatac cccctgttaa gaatatgagc gacacggatg agattgcgtc
     6481 tgcgaacaac tgtaagtgtg cgaagataac caggtttctt atccggggga ggaacaatcc
     6541 gtagacgacc gaaaggatat agagggggat gatgatgaag taaagaatgg tatgggtata
     6601 cggaaactta taaaagccta ttccaaagaa aaggaatgtt ccgagaagaa aagtgacaaa
     6661 aaaagccctg aaaactatca gggccttgag tctcttgaag agggcgttat caggcatacc
     6721 gtgattattt tatgagcgta atcattttga atatgggcaa atacatggcg actactataa
     6781 agccgatcgc acctccgaga aataccataa gcaggggctc catcattgcg gtgaggttcg
     6841 ccaccgcgtt atccacctca tcatcgtaga agtccgcaat cttgcccagc attgcatcca
     6901 gtgcgcctgt ggattcgccc actgcaatca tatgggtgac catgggagga aagacctttg
     6961 cctttgtaag gggctcctcc agagtctttc cctctgacac tgcctgtctt acatccataa
     7021 tagcgtactc tatcactttg ttcccggcgg tttttgcggt gatctcgagg ccatccagga
     7081 tagggactcc gctgcttatc agtgttccca gtgtcctggt gaattttgcc accgcaacct
     7141 tcctcaggag tatgccgaag atagggagtt ttagcgcgat cgaatcaatg acctttttcc
     7201 ccttttccgt ttttcttatc tggacgattg atacgattat cgccacgatc aggcctgcga
     7261 caataagccc gcctatgcct gcgatgaagt gactcatgcc tacaacaata cgtgtaggga
     7321 gggggagtgt gcctccgagg cttgcaaaca tctgagaaaa tgtgggtatt acaaagatca
     7381 taatgacggc gatgaccagt actgcaacgc tcgtgaccac tgcaggatag atcatagcgc
     7441 ccttgacctt cttcttcagc ttcattgcct tttcaatata cgcagcaagc ctgttcaata
     7501 ttgtatcgag gatacctcct gcttcccctg ctgctaccat gttgacatag agctctgaga
     7561 atattttcgg gtgttttcta agggcatcag cataggtgga accgccctcc acatccgcct
     7621 tgataaccgt aaggttcttt gcgaggcttt tattttccac ctgtgtagag aggatctcga
     7681 ggccctgtac gaggggaagc cctgcatcga tcatggtgga gaactgtctt gtaaatacga
     7741 cgatgtcttt atcactgact ccgccgccaa gacttaagcc gctaagcaat cctttcttgg
     7801 tttcagtctt ttcggtgacg acagtggcgg ttatgttttt ccgtctcagt tgagc
//
LOCUS       DATKOU010000019        11121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_408995, whole genome shotgun sequence.
ACCESSION   DATKOU010000019 DATKOU010000000
VERSION     DATKOU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408995"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1490)
                     /locus_tag="VMU21_00650"
     CDS             complement(294..1490)
                     /locus_tag="VMU21_00650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUO76060.1"
                     /translation="MDFLFTEEEELFRKSVRDFCGRRIEPRADEIDEKGEIPHEILKD
                     MGDTGLFGLTMSKEYGGSGQGFIKAAIAAEEIARADVSMAVAVYYLVETGWGFLLDRY
                     GSRNAKEDILPDVVKGKTFLGIASTEAAGGSDISSLSTRMEKREDVLVVNGAKAYISG
                     VREAALYGGGYVTIVKTDPQLGHKGLSLCYLPIKDKEGITISTSRQMGREGISNGFIR
                     IENVEIPGHYLIGEWNKGFYYAMEGFNCARTLVSAACIGAAEKALGLGIEYIKRRKLF
                     SSPLAKFEGIQFQLAEDYTRLESASLLVYKAAWMLDRMYSENRFSHQEINKAVATAKL
                     TAPTVAFDIIKNVMTWHGAYGYTKEAGLERGLRGVASYIVGAEGAQNIMRLIISREIL
                     GKEFMP"
     gene            1691..2539
                     /locus_tag="VMU21_00655"
     CDS             1691..2539
                     /locus_tag="VMU21_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HUO76061.1"
                     /translation="MEIRKIAVIGAGTMGSGIAQMTAQSGYEVVMEDVKDEYVRTGFT
                     KIKERLEKRVSEGKLEVREKEKVLSNIKVTTRLGGCKNADLIIEAVAEREEIKKEIYR
                     ELDTLCPEGTIFATNTSSLSITRLAQATGRPGRFVGMHFMNPAYSMKLIEIIRGLRTS
                     EETIDIVRAVAGRMGKVSVVVNDFPGFVISRVIATMINDAIYCLEEGVAPREGIDAIM
                     KFGANHPMGPLELADLMGLDICLEILEILQAELGEKYRPCPLLRKMVAAGKLGRKSGE
                     GFYEYR"
     gene            2564..4054
                     /locus_tag="VMU21_00660"
     CDS             2564..4054
                     /locus_tag="VMU21_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUO76062.1"
                     /translation="MSGLTKNYIGGRWVDASTGQTFEARNPADTDELVGVVVKSAKTD
                     VDWAVAAAQSAYRSWRLFPAPKRGEILFRAGELLLKRKQQLGELETREMGKVLAETLG
                     DVQEAIDMAYYMAGEGRRLSGETIPSELPDKDSKSMRVPLGVCGLITPWNFPIAIPAW
                     KIMPALVSGNTVVFKPSSPTSLAASKLVEILEEAGLPKGVLNLVHGRGEEMGEYLATH
                     PGIDGVSFTGSTAVGEKLAARCIELGKKVSCEMGGKNAIIVMDDANLDLAVEGAVWGG
                     FGTTGQRCTAASRVVVHKDVYESFLDMFTRAAKKLKLGNGLHKGTDVGPVINRVQMEK
                     VLSYVEIGSKEGAKLLTGGKAYLKGACARGYFIEPTVFSDVTSGMRIAREEIFGPVVS
                     VLKARDLEDAIAIVNDTKYGLVSSLYTQDVNKSAIAERELDTGIVYINASTIGAEIQL
                     PFGGTKKSGIGYREAGGRGGALDMFTKWKVVYRDYSGKLQKAQIDR"
     gene            4067..5350
                     /locus_tag="VMU21_00665"
     CDS             4067..5350
                     /locus_tag="VMU21_00665"
                     /inference="COORDINATES: protein motif:HMM:NF005993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUO76063.1"
                     /translation="MADELIERAKAVLTPALRIHTDIVVRKAYGIYVEDRDGRRYMDF
                     TSGLATTNIGHNHPRVVDAIKTQAESLVHSGCIFYYEPLTVLPEMLKKVTPPGIDMFF
                     FSNSGAEAVEGALKLARFHSEKQGIVAFTSCFHGRTFGAMSLTTSSIRYRRHYQPFVP
                     SIYHSPYPYCYRCFFDQKPDTCRMDCFAYLKRLFKHLIHPDEIACIIMEPVLGEGGYV
                     VPPAEFLKNLRALCDTWGILLILDEVQAGMGRTGKWFASEHFGVVPDIMTIAKGIASG
                     MPLSAVAASKEIMAKWSPGAHGTTFGGNPIGCAAAIATLSVIEEEGLLENAARVGRHT
                     LERLKTMQEKYPCIGDVRGLGLMLGIEFVKQGREPFTEELKKIMDYCLDKGLILVECG
                     VDKNVIRLAPPLIVTEEQIERGLDILGRAIAAVCG"
     gene            5420..6739
                     /locus_tag="VMU21_00670"
     CDS             5420..6739
                     /locus_tag="VMU21_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76064.1"
                     /translation="MTEGSKKEKVIIMGAGGRDFHNFNMLYRDNLQSEVVAFTAAQIP
                     YIEKRVYPPELAGPRYPEGIPIHPEPQLESLISSHRVNTAVFSYSDVSHEYVMHRASL
                     CLACGADFLLPGPEHTMLGSSVPVISVCAVRTGCGKSIITRKLAHAFKETGIGVSVIR
                     HPMAYCDFVPVKRFATMEDIDRGSCTIEEREEFEPLVEKGITVYAGIDYQKVLHDAEK
                     VSQVIIWDGGNNDFPFIRPDFEIVLIDALRPGHELLFYPGEVNLRRGNLLIITKINEA
                     KESDRQGIKAVIRRETPDAAVLETSSVFTISDYEAIKGKRALVIEDGPTITHGGMPYG
                     AGMAASSGIVSEFIDPRRYAAGSLQETYQKFSHIGPVLPAMGYSHTQRKDLEETISRV
                     PADVVIIATPVDLRRIIPIDKPTVRVFYDFDVDLSTLAGSFLKAHVR"
     gene            6729..7670
                     /locus_tag="VMU21_00675"
     CDS             6729..7670
                     /locus_tag="VMU21_00675"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HUO76065.1"
                     /translation="MCAEGSVLIALGGNALILPGERGYIEEQLRHADACMGPIAALIS
                     QGTRVVITHGNGPIVGNILLRNECAREYIPPMPLHICVADSQGGIGAMMAESLGNELR
                     KLGVDKTVSVIITHVVVARDDPAFKEPVKPIGPYYRESDAAKYIHEGWKMREIPGRGW
                     RRVVPSPKPREIVEMQGIRTAFEHGIIPIAAGGGGIPVVEEEGLLKGIDAVIDKDLTA
                     SRLASELGVERFVILTDVDGVYLHWETERKQRLERLTAKDAERYLSEGQFPSGSMGPK
                     IAAAIQFFQQGGRDAVIAKPEDLIAAMQGSAGTRISL"
     gene            complement(7945..8616)
                     /locus_tag="VMU21_00680"
     CDS             complement(7945..8616)
                     /locus_tag="VMU21_00680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUO76066.1"
                     /translation="MFPIRDTIPNRTFPSTTWSIIILCGIVFFFETTLPQEVLEEFTY
                     YFGVVPIEYTKRHRHGLSLIDYLSFLTTMFLHGGWLHFLGNMWFLKIFGSKLEDRMGH
                     GRFLVFYLISGILASMSYIYFSPQSSMPVIGASGAIAGVMGAYYVMFPHARILTLIPI
                     IIFPWFVELPAVFFLGWWFLLQVFSGTVAQVLPASDGGVAWWGHIGGFIVGIILMPFF
                     RRRYR"
     gene            8767..9423
                     /locus_tag="VMU21_00685"
     CDS             8767..9423
                     /locus_tag="VMU21_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HUO76067.1"
                     /translation="MKVIALNGSPRKKWNTATLLKKALEGAASQGAETELIHLYDLTF
                     KGCISCFACKTKDGKSYGRCAVEDDLTPVFRKIKKADAVILGSPIYFGSVSGEMRSFM
                     ERLLFPHLTYTDPPQSLFPRRIKTGFVYTMNTTEEQMKERGYVQLFSLHEVVLRMIFG
                     ASEYMCSFDTYQFDDYSKMVSVRFDPEKKAQRRREIFPKDCEKAFEMGARFARRESMK
                     "
     gene            9420..9749
                     /locus_tag="VMU21_00690"
     CDS             9420..9749
                     /locus_tag="VMU21_00690"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HUO76068.1"
                     /translation="MKKLFIALLMIVPFVSSLGNATAWERSFQWNWGPWGFGAGTFFL
                     IPIFMIIAFWVVVIIGIVYFVKWIIAAGKRHETKSEDTAIDILKKRYARGEISKEEFE
                     RIRQDIQ"
     gene            complement(9878..10063)
                     /gene="tatA"
                     /locus_tag="VMU21_00695"
     CDS             complement(9878..10063)
                     /gene="tatA"
                     /locus_tag="VMU21_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUO76069.1"
                     /translation="MFRGLFQPMHLLIILGIALVIVGPGKLPEIGKALGKSIQGFKKA
                     IAGDDKDSTVEEPQKKG"
     gene            complement(10218..10739)
                     /locus_tag="VMU21_00700"
     CDS             complement(10218..10739)
                     /locus_tag="VMU21_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUO76070.1"
                     /translation="MSKPGRNDPCPCGSGKKYKKCCLAEAFVRVGKEESIRERLVDNP
                     FELHNIDTSQDLTEANKAHEDRPPKTPESDIPMEMQEIQMERRTTTEPTSTAKKIMEL
                     FRYFRVKKGEYLSVKLIRSKQHLWKGIEEEEFNEAVSYLIHLGYIERTENPAGWKLLE
                     AGDDDLRQLPLHF"
     gene            complement(10774..>11121)
                     /locus_tag="VMU21_00705"
     CDS             complement(10774..>11121)
                     /locus_tag="VMU21_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HUO76071.1"
                     /translation="EVVNGLVTVFCQGSTGTITTIEFEPGVIQDLKKTLEKIVPSNIP
                     YEHDKAWGDGNGFSHVRAALMKPSLSIPVVDGKMSLGMWQQIVFSDFDNRPRERKVVV
                     QVIGHDGKEGNQG"
BASE COUNT         2879 a         2640 c         2921 g         2681 t
ORIGIN      
        1 tacctgactt tggcattgaa ggaaagacat gcactggtgg aaaaaatggc acaaaattac
       61 ccatttcttg ttgaagtcga gaccagccaa acagaggttg gatatgaatc gattggaaat
      121 gaagggatgc ggatgctata ggtagcacgg cattgggttg aggaaatcac cgaagggtag
      181 ttatttcacc gaccctgcct tcggtgtgtt cttttctccc taatgtggtt acatcagtct
      241 tgcatgggtc ctggatgcag gtgagcaagg tgcgggtgcg taccaggaat tcattaaggc
      301 ataaactcct ttcctaaaat ctccctgctg attatgagcc tcatgatgtt ctgggcgcct
      361 tcggcaccca caatatacga ggccacaccc ctcaaccccc gctccagtcc cgcctccttt
      421 gtatacccat acgccccgtg ccatgtcatc acattcttga taatgtcaaa ggcaacggtg
      481 ggggcagtga gcttcgcggt cgcaacagcc ttgtttatct cctgatggct gaacctgttt
      541 tcggaataca tccggtcaag catccaggcg gccttgtaca caagaaggct tgctgattcg
      601 agtctcgtat aatcctctgc cagctggaac tgtatccctt cgaactttgc gagaggagag
      661 ctgaaaagtt ttctcctttt tatatactct atgcctaacc ctaatgcctt ctcggctgca
      721 ccgatgcatg cagcggatac gagagtcctt gcgcagttaa atccctccat agcatagtaa
      781 aaacccttgt tccattctcc aatgagatag tgaccgggta tctcaacgtt ctctatcctg
      841 ataaagccat tggatattcc ttccctcccc atctgtctgc tggtgctgat agttatcccc
      901 tctttgtcct ttatcggaag ataacagagg gacaaaccct tatggccgag ttgggggtct
      961 gtcttcacta tggttacata accccctccg tacagggcag cctccctcac gccgcttatg
     1021 tatgcctttg caccgttcac caccagcaca tcctctctct tttccatgcg ggtggagagg
     1081 gaggatatat cagagcctcc tgccgcctct gtggaggcta ttccgagaaa ggtctttccc
     1141 tttaccacat caggaaggat atcttccttt gcattcctgc tcccgtatcg gtccaggagg
     1201 aaaccccaac cagtttccac cagataatac actgcaacag ccatgctcac atcggccctg
     1261 gctatctcct cggctgcgat cgctgctttg atgaaacctt gaccggagcc cccgtactcc
     1321 ttagacatgg tgagcccgaa aaggcccgtg tcccccatgt ctttcaggat ttcatggggg
     1381 atctcacctt tctcgtcaat ctcatccgcc cggggttcaa ttcttctccc gcagaaatcc
     1441 ctcacggatt tcctgaaaag ttcctcttct tcagtgaaca aaaagtccat agcaaaacct
     1501 ttaccccgtg agaataattt tccgtgctta gcgaacaaaa gactaatgcc ctgtgttcgc
     1561 ttttcccgtg aaaaaagaga aggttttctc acggggttta caccgtctga aattgagata
     1621 taattttatt aacactatac cacaaaaaga agtgagaccg aagaaagatg acgaaggtaa
     1681 tgaggagagc atggagataa gaaaaattgc ggtaataggc gcgggaacca tggggagcgg
     1741 tatagcacag atgacggcac agagcggcta tgaagtggtt atggaggatg tgaaagacga
     1801 atatgtaagg accggattca cgaagataaa ggagaggctt gaaaagaggg tgagcgaagg
     1861 gaaactggaa gtcagggaga aagagaaggt cctctccaat ataaaagtta ccacaaggct
     1921 cggtgggtgt aagaatgcgg acctgataat tgaggcagtg gcagaaaggg aggagatcaa
     1981 gaaagagatc tatagagagc ttgataccct ctgccccgaa ggaacgatct tcgcaacgaa
     2041 cacctcttcc ctctccatca caaggcttgc ccaggcgacc ggaagaccgg gccggttcgt
     2101 gggcatgcat tttatgaacc cggcatatag catgaaactc atagagataa tcaggggtct
     2161 ccgcacgtcc gaagaaacaa tagatatcgt gagggcagtt gccgggagga tgggaaaggt
     2221 gtctgttgtg gtgaatgatt tccccggctt tgtcattagc cgcgtgattg ccacgatgat
     2281 aaatgatgca atctactgcc tggaggaagg ggtcgctccc agagagggca tcgatgccat
     2341 aatgaaattc ggcgcaaacc atcccatggg acccctggaa ctggcggacc tcatgggcct
     2401 cgatatatgc ctcgagatcc ttgaaattct tcaggctgag ttaggagaga aatacagacc
     2461 ctgtccattg ctcagaaaga tggtagcagc cggaaagctg gggaggaaaa gtggggaggg
     2521 tttctatgaa tacaggtgag gggatatgag gggaggtgtg gttatgtcgg gcctgacaaa
     2581 aaattatatt ggcggcaggt gggttgatgc ttctaccggc cagacctttg aagccagaaa
     2641 ccctgcagat accgatgaac tggtcggggt ggtggtcaaa tcagcgaaga ccgatgtgga
     2701 ctgggcagtg gcagcggcac agtcagcata caggtcgtgg aggttgtttc ctgctcccaa
     2761 aagaggtgaa atactcttca gggcagggga gttgctgctg aaaagaaagc agcagcttgg
     2821 ggagctggag acgagggaaa tgggaaaggt ccttgcagag acccttggtg atgtccagga
     2881 ggccattgac atggcgtatt acatggcagg agaggggagg agactttcag gagagacgat
     2941 cccttcagag ctgccggaca aggactctaa atccatgcgt gttcctctcg gggtctgcgg
     3001 cctcatcact ccctggaatt ttcccatagc aatcccggca tggaagatca tgcctgccct
     3061 tgtgtccggg aataccgtgg tcttcaaacc aagcagtcct acttccctcg cagcatcgaa
     3121 gctcgtggag atactggagg aggcaggtct gcccaagggc gtcctgaacc ttgtccatgg
     3181 aaggggggaa gagatgggtg agtatcttgc cacccatccg gggatagacg gggtttcttt
     3241 taccggttcg actgctgtgg gcgaaaagct ggcagccagg tgtatcgaac tgggaaagaa
     3301 ggtgtcgtgt gaaatgggag gcaagaacgc cattatcgtg atggatgatg cgaatctgga
     3361 tctggcggtt gaaggggctg tatggggagg cttcggcact acaggacagc ggtgtactgc
     3421 cgcaagccgt gtcgttgttc ataaggatgt gtatgaaagc ttcctggaca tgttcacaag
     3481 ggctgcaaag aagctgaagc tcggcaacgg tcttcataag gggactgatg tgggccctgt
     3541 gatcaacagg gtccagatgg agaaggtcct cagctacgtg gaaatcggga gtaaagaggg
     3601 cgcgaagctc ctcaccgggg gcaaggctta cctgaaaggt gcgtgcgcga ggggctactt
     3661 catagaaccg actgtattct cggatgtgac gtccgggatg aggatcgccc gtgaagagat
     3721 cttcgggcct gtggtttctg tcctgaaggc cagggacctg gaagacgcca tagccatcgt
     3781 caacgacacg aagtatgggc tcgtctcttc cctctatacc caggatgtga acaagtcagc
     3841 cattgcagaa agggaacttg atacaggcat tgtatacata aatgcatcga ccatcggtgc
     3901 tgagatccag cttccctttg gcgggacaaa gaagagtggt atcggctaca gggaagccgg
     3961 ggggagggga ggcgctctgg acatgttcac caaatggaag gttgtttacc gcgactacag
     4021 cggaaagctc cagaaggcac aaatcgaccg ataagagagg atatacatgg ctgacgaact
     4081 tatagaaaga gcaaaggcgg tcctcacccc tgccctcagg atccacacgg atattgtggt
     4141 gagaaaagcc tatggaatct atgtagagga cagagacggc aggcggtata tggatttcac
     4201 ctcagggctg gcgaccacca acataggcca caaccatccc cgggtggttg acgccatcaa
     4261 gacccaggct gaatcccttg tccattcggg ctgcatcttc tactatgaac cacttacagt
     4321 acttccggag atgctcaaga aggtgacacc cccgggcatc gatatgttct ttttcagcaa
     4381 ctcgggtgct gaggcagtgg agggcgccct caagcttgcc agatttcatt ccgaaaaaca
     4441 gggcatcgtc gcattcacca gctgtttcca tggaaggacc tttggcgcca tgagcctcac
     4501 cacatcgagc atacgctaca gaaggcacta ccagcccttt gtcccttcca tttaccactc
     4561 accctatccc tactgctacc gctgcttctt tgaccagaag cccgatacct gcagaatgga
     4621 ttgctttgca tacctcaaga ggttatttaa acacctcatc catccagacg agatcgcctg
     4681 cattatcatg gagcctgttc tcggtgaagg gggatatgtg gtcccccctg cagaattcct
     4741 aaagaatctg agggccctat gtgacacgtg gggcatcctg cttatcctcg atgaggtcca
     4801 ggcaggcatg ggaaggaccg ggaaatggtt tgcctcagaa cattttggtg ttgtccctga
     4861 tattatgacc attgcgaagg ggatagcgtc gggcatgcct ttgagcgctg tggcggccag
     4921 taaagagatc atggcgaaat ggtcgcccgg cgcccatggg accacctttg gcgggaaccc
     4981 catcggctgt gctgctgcca ttgcaacgct cagcgttatt gaagaggagg ggcttctgga
     5041 gaatgccgcg agagtgggga gacacaccct tgaaaggctc aagacaatgc aggagaaata
     5101 cccctgcata ggagatgtga gggggctcgg tctcatgctg ggaatagagt ttgtgaagca
     5161 aggcagggag cccttcaccg aagagttgaa gaagatcatg gattactgcc tcgataaggg
     5221 attgatactc gtggagtgcg gcgtggacaa gaacgtgata cgccttgcgc cgccattgat
     5281 cgtaacagag gagcagatag agagagggct cgatattttg ggaagggcaa tcgcggctgt
     5341 ctgcggataa tctatcgtcc caggcttttt cgtctgccat gcctgctttc atttttaaga
     5401 cagagggatt gccgcgaata tgactgaagg atcgaaaaaa gagaaggtca ttatcatggg
     5461 agcaggaggc agggacttcc ataacttcaa catgctctac agggacaatc ttcagagtga
     5521 agttgtggcc tttaccgctg cccagatacc ctacatagaa aaaagggtct acccaccgga
     5581 acttgctggt ccgcgctatc ccgaagggat acccatacat cccgagccgc aactggaatc
     5641 attaatctca tcacataggg tgaacacggc agtattttcc tacagcgatg tttcccatga
     5701 atatgtcatg cacagggcct cgctctgcct tgcctgcggc gcggatttcc tcctgcccgg
     5761 acctgaacac accatgcttg ggtcatcagt tccggtcatc tcagtctgtg cggtgaggac
     5821 cgggtgcggt aagtcaatca tcacgaggaa gctggcccat gcattcaaag aaaccggaat
     5881 cggggtgtct gtgatacgcc atcccatggc gtactgtgat tttgtccctg tgaagaggtt
     5941 tgctaccatg gaggatattg accggggttc gtgcaccatc gaagagaggg aagagttcga
     6001 gcccctcgtg gagaaaggca tcacggtcta tgccggcatc gactaccaga aggtcctcca
     6061 tgatgcggaa aaggtctctc aggtcatcat ctgggacggc gggaacaatg actttccctt
     6121 catacgtcct gatttcgaga tcgttctcat tgatgcctta aggcctgggc atgagctgct
     6181 cttttaccca ggcgaagtga acttaaggag gggaaatctt ctcatcataa ccaagataaa
     6241 cgaggcaaag gagagtgaca ggcaaggaat aaaggccgtt attcggaggg agactcccga
     6301 tgccgcagtt cttgagacca gctctgtttt tactatcagt gattacgaag caataaaagg
     6361 gaagagagcc ctggtgatag aggatggacc aactattacc catggaggga tgccttatgg
     6421 ggccgggatg gccgcgtcca gtggtatcgt gtcggagttc attgacccga ggcgctatgc
     6481 agcgggttcc cttcaggaga cgtatcagaa gttttctcac ataggtccgg tgcttcctgc
     6541 catgggatac tcccatacgc agaggaaaga tctggaagag accatctccc gggtccctgc
     6601 tgacgtggta attattgcca cgcctgtgga cctcagaagg atcataccca tcgacaaacc
     6661 gacagtgcgg gtcttttatg atttcgatgt tgatctcagc actctggcag gatcatttct
     6721 gaaggcccat gtgcgctgaa ggcagcgtgc tcatagccct gggcggcaat gccctcatcc
     6781 ttcctggtga gaggggctat atcgaagagc agctgcgcca tgccgatgcc tgtatggggc
     6841 cgattgcagc cctcatctca cagggaacac gggtcgtcat tacccacgga aacggaccca
     6901 ttgtggggaa tatccttctc aggaacgaat gtgccaggga gtacatccct cccatgcccc
     6961 tccatatctg cgttgctgac agccaggggg ggatcggcgc catgatggcg gaaagccttg
     7021 gcaatgaact gaggaaactc ggtgttgata aaaccgtctc agttatcatc acccacgtcg
     7081 ttgtagcaag agacgacccc gcattcaaag aacccgttaa acccataggc ccttactacc
     7141 gggagagtga tgcagcgaaa tatattcacg agggatggaa gatgcgggag atcccggggc
     7201 ggggttggag gagggttgta ccgtcaccga agccccggga gatcgtggag atgcagggga
     7261 taaggaccgc cttcgaacat ggcattatcc ccattgctgc gggaggcgga ggcatcccgg
     7321 tggtggaaga agaggggtta ctgaaaggca ttgatgcggt catagacaag gacctgaccg
     7381 caagtcgtct cgcctctgaa ttaggcgtcg agagatttgt gatccttact gatgtggatg
     7441 gcgtgtatct tcactgggag acggaaagaa agcagagact tgagaggctt acagcgaaag
     7501 atgcggagag atacctgagc gagggacagt tcccgtccgg cagcatgggg ccgaagatag
     7561 cagcagccat ccagtttttc caacagggag gaagggatgc cgttatcgca aaacctgaag
     7621 atttaatagc ggcaatgcag ggcagtgccg gaacgaggat aagtttgtga acgacgcgat
     7681 aaactcgtac aaaaaacttg acaaccatat caaatggtgc tagcctggaa tcagaagaag
     7741 tcaatgcctt gggatgggtt aaggcttaga accaagtaaa tgggttaggg agcgggagtc
     7801 agatgtggtg agcaagcctc cagcagcgag gaggaagcct aaaacatctg ctgaagcacc
     7861 cacttaaaaa caatctaatg catacccaga acccaaggta ttgcacttct tgttttttta
     7921 tgattcccta tacccgattc tctttcagcg gtatctccgc ctgaaaaatg gcatcaggat
     7981 aatcccaaca ataaaacccc caatgtgccc ccaccatgct actccaccgt cagatgcagg
     8041 aagcacttgg gctacagtac ccgaaaatac ctgcagtaag aaccaccacc ccaaaaaaaa
     8101 tactgccggc agttctacaa accaggggaa tatgatgatg gggatcagtg tcaggattct
     8161 cgcatggggg aacattacat agtatgcccc cataacaccc gcaatcgccc cagaggcacc
     8221 gattacaggc atagatgact gggggctgaa gtagatgtag gacatgcttg ccaggattcc
     8281 tgaaatcaaa tagaacacaa gaaatcggcc atgtcccatg cggtcttcaa gcttactgcc
     8341 aaatatcttg agaaaccaca tattccccag gaaatggagc catccgccat gcaggaacat
     8401 cgtggtcagg aatgaaaggt aatcaatcag ggaaaggcca tgacggtggc gtttggtata
     8461 ttcaatagga acaaccccaa aataatatgt gaattcctca aggacttcct gtggtaatgt
     8521 agtttcgaag aaaaagacaa tcccgcacag tatgatgatg ctccacgtcg tggaaggaaa
     8581 tgttctgttc ggtatcgtgt ctcttatggg gaacatccgg tctgtctcct acgggagtaa
     8641 agcctcttca tatgatcctc accttttttt attgtcaagt attttacaac aggctacatg
     8701 gtaaaatatt cttgtcacga cggatcaagg aggctgtcgt caatccagtt gaaaggagct
     8761 ataacgatga aggttattgc attgaatgga agtccaagga aaaaatggaa cacggcgaca
     8821 ttgcttaaga aggcccttga gggagctgcc tcacagggag cagaaacaga actcattcat
     8881 ctctatgatc taacctttaa aggatgcata agctgttttg cctgtaagac caaagatggc
     8941 aagagttatg gcaggtgtgc agtcgaggat gacctgacac cagtctttag aaaaattaag
     9001 aaggccgatg cggtcatctt gggctcaccg atctattttg gaagtgtttc tggagagatg
     9061 agatcattta tggagcgcct cttgtttcct cacctgacat acaccgatcc tccacaatcg
     9121 ctttttccca gaaggattaa gaccgggttt gtgtatacaa tgaataccac agaggagcag
     9181 atgaaagaac gtggctacgt tcagctcttc agtcttcatg aagtggtact aaggatgata
     9241 ttcggggctt ctgagtatat gtgcagcttt gatacttatc agtttgatga ttattcaaag
     9301 atggtttcag tccgttttga tcctgagaag aaagcacaga ggcgcaggga aatatttcca
     9361 aaggattgcg aaaaagcctt tgagatgggg gctagatttg ccagaaggga aagtatgaaa
     9421 tgaaaaaact ttttattgcc ttgttaatga tagttccgtt tgtatcatcc cttggtaacg
     9481 ctacagcatg ggaaaggagt tttcaatgga actggggacc ctggggtttt ggagctggca
     9541 cttttttctt gataccaata tttatgataa ttgccttttg ggttgttgtt attatcggga
     9601 ttgtctattt tgtcaaatgg attatagcag cggggaagag gcatgaaaca aaatcagagg
     9661 acacagcaat tgatatcttg aagaaacggt atgccagggg agagatttcc aaggaggaat
     9721 ttgaaaggat aaggcaggac attcaatagg tcttaggctt tcatcagtaa aataatcaag
     9781 aaacctatca gcaagactct ctcaaaccga tgcctccgag agtgagcagg ataaaaagcc
     9841 cgatagaggg gtaattagct ataaggactg taatatatta ccccttcttt tgtggctctt
     9901 ctacagttga atctttatca tccccagcga tagccttctt aaaaccctgt atgcttttgc
     9961 ccagcgcttt accgatttct ggtagttttc ctggaccaac tatcaccagt gctatcccaa
    10021 gtattattag cagatgcatc ggttggaaaa gtcctctaaa catagtttca cctccctcca
    10081 cattgtagac tcccaatatt gatattataa ccaaacgcca ctttttctat tatcgatttg
    10141 tcatgttacc cgctaaagca agctcttagt ggattccatc cttttcccct ctctctttga
    10201 aatgttaatc cgcattttca gaaatgcaga ggaagttgtc tcaggtcatc atcacccgcc
    10261 tcaagaagtt tccagcccgc cgggttttct gtcctctcta tgtaacccag atgtattaag
    10321 taactcacag cctcattgaa ttcttcttcc tcaataccct tccacaggtg ttgtttgctc
    10381 cgtatcaatt ttaccgatag gtattcaccc ttctttaccc tgaaatacct aaatagttcc
    10441 atgattttct tggcagtgga tgtgggttca gtcgttgttc gcctttccat ctgaatctcc
    10501 tgcatttcca tcgggatatc actctcaggg gtcttggggg gccgatcttc atgtgccttg
    10561 tttgcctctg tcaggtcttg ggaagtatct atattgtgga gctcaaatgg attatcaaca
    10621 agacgttccc tgatggactc ttccttgccg acccgtacaa aggcctcggc aaggcaacac
    10681 ttcttatact tcttccctga tccacaaggg caggggtcat ttctgcctgg tttgctcatt
    10741 cctggtggtc ccttctataa tattgcctat aagttatccc tggtttcctt cttttccatc
    10801 atgcccaatg acctgcacca caacttttct ttctcttggt ctgttatcaa agtcgctaaa
    10861 aacgatctgt tgccacatcc ccaacgacat cttgccgtct accacaggga ttgagaggga
    10921 aggcttcatc aatgcagccc tgacatgaga gaaaccgttt ccgtctcccc atgccttgtc
    10981 atgctcatag ggaatattgg aaggcacaat cttttccaat gtttttttga ggtcctggat
    11041 gaccccaggt tcaaattcta tagtggttat ggttcctgtt gatccctgac aaaaaaccgt
    11101 gacaagacca ttcaccacct c
//
LOCUS       DATKOU010000020         9773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4642102, whole genome shotgun sequence.
ACCESSION   DATKOU010000020 DATKOU010000000
VERSION     DATKOU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642102"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VMU21_00710"
     CDS             complement(<1..343)
                     /locus_tag="VMU21_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUO76072.1"
                     /translation="MKTTFRTISNDDVVAGGQRAPEPNAVDDDELLDSYSRAVISAAD
                     KVSPSVVHIGVQQGSQGTGSGFIFTPDGFILTNSHVVHNAKRIEVTLPDGRHYQAYTV
                     GDDPDTDLAVIR"
     gene            complement(578..934)
                     /locus_tag="VMU21_00715"
     CDS             complement(578..934)
                     /locus_tag="VMU21_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76073.1"
                     /translation="MTAAKAEKMQQRLDAGLVSELFPKVTEIVISMQYSQTGVLEPLS
                     RTVNFFPGSSATFKVNCLCSECVESGFDFTKIITEMVKTHKTGSKGKIDCESCSAPEC
                     SDVAYSVTIKYAHIMK"
     gene            complement(1007..1228)
                     /locus_tag="VMU21_00720"
     CDS             complement(1007..1228)
                     /locus_tag="VMU21_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HUO76074.1"
                     /translation="MEKEVKKVAQGTVKWFNESKGFGFVTAEDGTDVFVHHTSIQGSG
                     FKTLAEGDKVSFDIEKGPKGPKAINVVKL"
     gene            complement(1378..1959)
                     /gene="msrB"
                     /locus_tag="VMU21_00725"
     CDS             complement(1378..1959)
                     /gene="msrB"
                     /locus_tag="VMU21_00725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HUO76075.1"
                     /translation="MLEFVKKIMVFGGVLMMFSISVVNAEKSEQATSKGSSAIASTEK
                     WRTFVKPSQEELKKKLTPIQYKVTQKNDTEPAFKNEYWNNERDGIYVDIVSGEPLFSS
                     RDKYESGTGWPSFTKPLEPGNIVEKTDRSLFFIKRTEVRSKYGDSHLGHVFNDGPKPT
                     GLRYCMNSAALRFIPKEDLAKEGYEEYLRLFMK"
     gene            complement(2017..2625)
                     /gene="msrA"
                     /locus_tag="VMU21_00730"
     CDS             complement(2017..2625)
                     /gene="msrA"
                     /locus_tag="VMU21_00730"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HUO76076.1"
                     /translation="MKYSILFLTMLTAGFITITTTMAATDLAKATFAGGCFWCMEHPF
                     DEIPGVISVTSGYTGGQKKNPTYKEVSAGGTGHAESVQIVYDPAKVTYEKLLNVFWHN
                     IDPTAKDRQFCDSGHQYRSAIFYHNEEQHRLALESKAQLEKNKPFKEPIVTEIVQATE
                     FYPAEDYHQHYYKKNPIRYKFYRTNCGRDHRLKELWGDAAGH"
     gene            complement(2691..2900)
                     /locus_tag="VMU21_00735"
     CDS             complement(2691..2900)
                     /locus_tag="VMU21_00735"
                     /inference="COORDINATES: protein motif:HMM:NF045255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76077.1"
                     /translation="MKEFRAIYPDMKSFIDSTAVARVNGYLGGYGSCDSIEEEIEGFG
                     LSQRAKGTLTSVVCNRKASMTCPVR"
     gene            complement(2937..3284)
                     /locus_tag="VMU21_00740"
                     /pseudo
     CDS             complement(2937..3284)
                     /locus_tag="VMU21_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(3887..4864)
                     /locus_tag="VMU21_00745"
     CDS             complement(3887..4864)
                     /locus_tag="VMU21_00745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HUO76078.1"
                     /translation="MLRTFPMFFLPICFLFPCQATADDVVRSRAAIVIDASTGRVLYG
                     VNPTLRLPPASTTKLMTALVTLDHRSPSETTVISENVSAVSPVKAIFKEGERVTVETL
                     LTAALIKSANDAAFALAETVGGTESHFVEMMNEKAAALGLADTRFINATGLPGDGQYI
                     TASDLANLLRQALGNPMIRDIVNAKVGSIHTESKGDMVLSNTNKLLWIDDSVFGGKTG
                     YTREARNCLVFASRRGDEVVIVSLLGADTRLHLWGEAEKLSAKGFSILAGEEKSHVYS
                     TRSAYGPYVERVAHKKRVNHTGGNAHKPRKKHWSKTRGSSPKTIEESSL"
     gene            5119..5775
                     /locus_tag="VMU21_00750"
     CDS             5119..5775
                     /locus_tag="VMU21_00750"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76079.1"
                     /translation="MKDKRQYPRYVVEGVEGTLLFTTHVDILNISISGVALKANRRLE
                     IGRQYALKLEYKDRTVSVNGVVVWSLLSELADGPHAEKVPVYKAGMRFTDVISDKMSR
                     LLEFIENHKLSQDERLNIRFTIKVPDKAILDGPHNYDVKNMSISGMLIETDMRFDVDA
                     RYPMEIALEGVRSIKVLGRVVSCLEVNDRAPKHYAIGIEFIEISDEDKKKLKEFVDTL
                     "
     gene            complement(5785..6420)
                     /locus_tag="VMU21_00755"
     CDS             complement(5785..6420)
                     /locus_tag="VMU21_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HUO76080.1"
                     /translation="MARNAHTFFTHKEKERIQATTKGVESRTIGEVAVMVVDSSDHYF
                     EAEVTGGILLGSLLSLWITASYFHSSVWWYVPLSFGLFFPSRLLFRRVPALKAAFIGL
                     HRKEHAVRLRAVRAFHERGLHKTKKNTGVLFFISLLERKVRILADSAIHEKIGQETLD
                     KFARHVSQGIKEGRGCDALCEAIQETGDLLARYFPLTKDDTDELRNDLITE"
     gene            complement(6420..7313)
                     /locus_tag="VMU21_00760"
     CDS             complement(6420..7313)
                     /locus_tag="VMU21_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HUO76081.1"
                     /translation="MRRGLRASLALFILLLLCSSSAHALDVPKLRGYVNDYANIISPP
                     ARTELEKELRSFEETDSTQIVILTIPSLEGEVMEEFSIRVADAWKIGQKGKDNGIIFI
                     VAKQERKMRIEVGRGLEGRMTDLMAGRIVDLVVKPRFKRGDFDGGFLAGVSSLIDATR
                     GEFKVDVTQRPARRERSSQLFTFLIFGGIALLILGSISRILGGAAGAIALPSIAHFTG
                     FSLGLGAAAVLALLGFGAGLLLPLLFSAGGRYRGGGVWPGGGFFGTGGSSGGGGFSGG
                     GSDFGGGGGGGFGGGGASGDW"
     gene            complement(7350..7937)
                     /locus_tag="VMU21_00765"
     CDS             complement(7350..7937)
                     /locus_tag="VMU21_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUO76082.1"
                     /translation="MKRPVLCLFSLVFLFTVSGCGYNTMQANEEAVKAAWGDVEASYQ
                     RRNDLIPNLVEVVKGYAKHERETLTAVTEARAKVGTVQVSKNIIDDPRAFAQFQEAQG
                     AMSSALSRLMVVVEKYPDLKANQSFQDLQHQLEGTENRINVARTRYNKAVEVFNTSLR
                     IFPNSLTNSLLLHLKLKEPFKAEAGAEKAPKVQFQ"
     gene            8051..8956
                     /locus_tag="VMU21_00770"
     CDS             8051..8956
                     /locus_tag="VMU21_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HUO76083.1"
                     /translation="MVLFISVRIRDFFLAVCLIFVLAPRLDAASSYSYGKGNMVIGEV
                     QTYEVEGDESLIEIARDFDVGYNQIESANPTLDPFVPGEEALVTVPTSWILPELISYD
                     GIVINISEMRLYYFHKMNRSPVVTTYPIGIGSEGNDTPVGVFKIIQKIVDPPWYPPPS
                     IRKERPELPKVIPPGPDNPLGSHALRLSLGTVLIHGTNRPFAVGRKVTHGCIRLYPED
                     IPQLFSVIPVGMKVTIVRQPIKVGVRDKKVYLEVHKDDYRQKVDYFNEAVKLLRKKGL
                     LKDVNTEKIYQAVQEKQGFPVEINQ"
     gene            complement(9013..9411)
                     /locus_tag="VMU21_00775"
     CDS             complement(9013..9411)
                     /locus_tag="VMU21_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76084.1"
                     /translation="MRKTVIMFLVVSLLSIFAFGCVTKDYVKQQIDPLVDRISKLESR
                     VSAVESKLSALDQKVGSMAADIDQAKKEAASAKALAQDAMKKATECCDKAEAAAKRAE
                     AAAARAEAAAARCEAAASKEVKAFELKQMK"
BASE COUNT         2376 a         2515 c         2318 g         2564 t
ORIGIN      
        1 ttcttataac cgcaaggtcg gtatccgggt catcacccac agtataggcc tgataatgcc
       61 ttccatcagg aagagtcacc tcaatcctct tagcattgtg caccacatgg ctgtttgtga
      121 ggataaaccc atccggtgta aatatgaagc ctgagccggt gccctgactc ccctgctgga
      181 cacctatgtg gacaacggag gggcttactt tatcagcagc gctgattact gcccttgaat
      241 acgaatcgag cagttcatcg tcgtctaccg cattcggctc aggggcacgc tgaccccccg
      301 ccacgacatc gtcattggat atggtgcgga atgtagtttt catcgtcttc gctccttttc
      361 ttatagtaac acaccatgta ttggttcctg gaacgtagcg gtgaaagtaa gcagggccgg
      421 caagacgtca actgcgaagt tcactggaga caggaagtaa gtcctttggc tcattgtcat
      481 cgaggtgcat gtcgattccc atggctgctc aataagcggc cttctttaag tgagtcatcg
      541 gaacgagtcg aagcgtcagc gatacacagg gacacaatca tttcataata tgtgcatatt
      601 ttatcgttac ggagtatgca acatctgagc attcgggagc agagcaactc tcacaatcta
      661 tttttccttt tgatcctgtt ttgtgggttt tgaccatctc agttataatt ttggtgaaat
      721 cgaacccgct ttcaacacat tccgaacata aacagttcac cttgaatgta gcagaactac
      781 cggggaagaa gtttactgtc cgtgacaaag gctccaatac cccagtttga ctgtactgca
      841 tactaataac gatttctgta accttaggga aaagctcaga cacaagacct gcatcaagtc
      901 tctgctgcat cttttcagcc tttgccgccg tcatcttcgc cctgcgattc atcttgcccc
      961 caaataaaaa ggccccctcc actaagaaag ggccttccat aggtaattac agtttaacga
     1021 catttatcgc tttgggacct ttcgggcctt tttcaatatc aaagctgacc ttgtcacctt
     1081 cagcaagagt cttaaaacca cttccttgga tggacgtatg gtgcacgaaa acatcagtgc
     1141 cgtcttcggc ggtaacgaag ccaaaaccct tactctcgtt aaaccatttt accgtacctt
     1201 gtgccacttt ctttacctcc ttctccatag acaaaatctc gaaatgctcc ccaaaaggcc
     1261 gcgaagtgaa aatagcatgt cctcaaacaa ttcgtcaagg aagaccaata atgttaaaac
     1321 tgtgataggg aatgattgcc gatggcggtc ttcttatttg tctactgccc gtggagacta
     1381 tttcatgaaa agtctcagat attcctcgta cccctctttc gcaaggtcct ccttcggaat
     1441 gaaccgaagg gctgcagaat tcatgcagta cctgagccct gtgggcttcg gcccgtcatt
     1501 aaagacatgc cccagatggg agtccccata tttactccgg acttcggtcc tctttatgaa
     1561 gaagagactt ctgtcagtct tttcaacaat gtttcctggc tcaaggggct tggtaaagct
     1621 tggccagccg gtaccggatt cgtacttgtc gcgggaactg aagagtggct ctccggacac
     1681 aatgtccaca tagattccgt ctcgctcgtt gttccagtat tcgttcttga aggccggctc
     1741 tgtgtcgttc ttctgagtca ctttgtactg tatcggcgtc agtttctttt tgagctcttc
     1801 ctgtgatggt ttcacaaatg tcctccattt ttctgttgaa gcaatagctg aagatccctt
     1861 agaagtcgct tgttcgcttt tctcggcatt aacaaccgaa atggagaaca tcattaacac
     1921 tccgccgaag accattattt tttttacaaa ttccagcatg attacctcct ttttctgtta
     1981 tagtccatga ttaaatatct cctcaatgat actgtgtcaa tgaccggccg catcgcccca
     2041 aagttccttg agccgatggt cgcgaccgca gttagttcgg tagaacttgt aacggatggg
     2101 gttcttcttg taataatgct ggtggtaatc ctctgcaggg tagaactcgg tggcctgcac
     2161 aatctcggtt acgataggtt ctttaaaggg cttattcttt tccagttgtg ccttagactc
     2221 cagagccagc cggtgctgtt cctcattatg ataaaagatg gcgcttcgat actgatgtcc
     2281 agaatcgcag aactgcctgt ccttagcggt cgggtcgata ttatgccaaa aaacgttcaa
     2341 gagcttttca taggtcactt ttgccggatc atagactatc tgaactgact cggcatgtcc
     2401 ggtcccacct gccgaaacct ctttataagt gggattcttc ttctgcccac cggtgtagcc
     2461 ggaagtaacc gaaatcacgc cgggaatctc gtcaaaggga tgctccatgc accaaaagca
     2521 gccgcctgcg aaggtcgctt tcgcaagatc ggtcgcagcc atggttgtag tgattgtaat
     2581 aaatcctgcc gttagcattg tcagaaaaag gattgaatat ttcatctgat acctcctctt
     2641 aagcagatgg atccatacgc tatcttcccg atcagcagta ataatgtttg ttaccgcacc
     2701 gggcatgtca ttgatgcttt gcggttgcag acaacagaag tcaatgttcc tttcgctctt
     2761 tgtgaaagtc cgaagccctc aatctcttct tcgatggaat cgcatgagcc atatccacca
     2821 aggtatccat taacacgtgc gaccgccgtt gaatcgatga aactcttcat gtcaggatag
     2881 attgccctga actccttcat tacttcggga tataagctca gactgtgttt ctggtgataa
     2941 tcctccgcac tatagaatcc cgaatacggt tctataacgg tgacaattct tccctttgtt
     3001 tccgaagcaa ggtgatctct cgatgctatt gcaaacttcc tttgttcttc gttgtggtag
     3061 aatatggccg ccctgtactg gcgggaccat gaatctcttg tcggatcatg acccttccag
     3121 aagacatcca gcaactccct gtatgaggtg cgggtagaat caaaatcgat ttgaaccgac
     3181 tcggagtgat cacccagatc gtgataaccc ggattcttcg tagtaccgcc ggcgtatccc
     3241 actcgggttc ggatgacccc tggcaagctc ccaaactggg agtcaggacc ccagaatcag
     3301 ccaagggcaa acgtagctgt ttcgaatctg cctggaagat gggtgtctaa aagtggcaga
     3361 ccaatactgg cctctgtatc gttattcagc atagacatgt tcttacctcc tttaagaact
     3421 accagggtaa cagcggatat tgctaaccct aacagaacca tgtaagcaaa gcgcgatttc
     3481 acaatatagt gtaccctata ttaatgaaga aaatatgaaa atagttctgt ctgttactta
     3541 atattttcca gatggctata gtggacgttt tcagatgttc ttgaccgggc ctgattgccg
     3601 cggttcagtc aattatgcag tgatttccgt caccagttta tccttaatat ctatgcaccc
     3661 tataacactc tgtgctgctg cacagagatg aaggtaggat gaaaaagctt cacgtgcagc
     3721 ttctcccgta cgatagtaat gcgtgatgtg tggacaattt atccatgttc tcctccatgg
     3781 ataaagccgg attaaacggc atttttttaa tggaaaccta gtttgataat tgcgcagcag
     3841 aacggcaccg tctaaggcag atatgagtcg ctgcgaaaat tagcgcctac agggaactct
     3901 cctctatagt cttcggagaa cttccccgcg ttttactcca gtgctttttg cgtggcttat
     3961 gagcattccc tcctgtgtga ttcacgcgtt tcttatgcgc gactcgctca acatacggcc
     4021 cataagcaga ccgtgtggaa taaacatgcg acttctcttc tccggcgagg atggaaaagc
     4081 ctttagcgct cagcttttca gcctcacccc acaggtgtaa tcttgtatcc gctcccagca
     4141 aagagacgat gacaacctca tccccacgac gactggcaaa tacaaggcag ttgcgtgctt
     4201 ccctcgtata gcctgtcttg ccaccaaaca ctgaatcgtc aatccataac aacttatttg
     4261 tgttgctgag aaccatatcc cccttgctct ccgtgtggat gcttcccacc tttgcattca
     4321 caatatcacg gatcattgga ttgccaaggg cctgtctcaa gagatttgca aggtcggagg
     4381 cagtaatata ttgtccatct ccaggcagac cggtagcgtt gatgaaacgt gtatctgcca
     4441 gccccagggc cgcagccttc tcattcatca tctccacaaa atgagattca gtcccgccca
     4501 ctgtctcggc cagtgcaaag gctgcatcgt ttgcggattt gattagtgcg gccgtgagca
     4561 gggtctcgac ggtaacccgt tcgccttcct tgaagatggc cttcacagga gataccgctg
     4621 agacattttc gctgatgacg gttgtctctg agggtgatcg atgatccagc gtaaccaagg
     4681 ccgtcatgag cttcgtcgtg cttgcgggcg gcagtctgag ggtggggtta actccgtaaa
     4741 ggaccctgcc tgtggaggca tcgatcacga tagccgcacg ggaccggaca acatcatcag
     4801 cggtggcttg gcaggggaac aaaaaacata ttggaaggaa aaacatcgga aatgttctca
     4861 gcatgcgtct tatcacaggt cccttcatat aggatattat agccgagaaa taatgagaat
     4921 gcgataccca tggtaaaaat acttggttca tgtcgcaatc tgcaaaccca ttgtccagac
     4981 atatgcatgc ggggactgcc gctcttcagg tgagaaagga atctctccgt ttcgtataag
     5041 gaataagatc aggcccgaga tcccttcttg tggggtgggc tttttgcaat ggaaaaatga
     5101 tctgtgataa gatgtctcat gaaggacaag agacagtacc cgcgatatgt ggtggagggc
     5161 gtagagggca ccctcctgtt taccacccat gtggatatcc tcaatatcag cataagcggc
     5221 gttgccctga aggccaatag gaggctcgag atcggaaggc agtacgcatt aaagctcgag
     5281 tacaaggaca ggaccgtttc ggtgaacggc gttgtggtct ggtctcttct cagcgagctc
     5341 gccgacggcc cgcatgcgga aaaggttccg gtatacaagg cgggaatgag gttcactgat
     5401 gtcatatccg acaaaatgtc gagactcctt gagtttatcg aaaaccacaa gctctcccag
     5461 gatgagaggc ttaatattag gttcaccata aaggtacctg acaaggcgat cctggatggc
     5521 ccccataact atgacgtgaa aaacatgagc atcagcggca tgctcataga gactgatatg
     5581 cggtttgacg ttgacgcaag atatcccatg gagatagccc ttgagggtgt caggagcatc
     5641 aaggtcctgg ggagggtcgt ctcatgcctt gaggtgaacg atagggcccc gaagcactat
     5701 gccatcggca tagaatttat cgagatttct gatgaggaca agaaaaaact gaaggaattc
     5761 gtcgacaccc tttgaacggg cctctcactc ggttatcaga tcattgcgga gttcatccgt
     5821 gtcgtctttc gtaaggggaa aatatctggc gaggaggtcc cctgtctcct ggattgcctc
     5881 gcagagggca tcacagccac gaccttcctt tatcccctgg gaaacatggc gtgcgaattt
     5941 atccagcgtt tcctgtccta tcttctcatg aatggcgctg tcagcgagga tcctcacctt
     6001 cctttccagc agtgagatga aaaaaaggac acctgtattt ttttttgttt tgtgaagacc
     6061 tctctcatgg aatgccctga ccgccctgag ccttacggcg tgttcttttc tgtgcaggcc
     6121 tatgaatgca gccttcaacg caggtactct tctgaaaagg aggcgtgacg gaaaaaagag
     6181 cccgaaactc aggggcacat accaccacac cgaagaatga aaatatgagg ctgttatcca
     6241 gagggagagg aggcttccga ggagtatgcc tcctgtcacc tcagcctcga aatagtggtc
     6301 gctgctgtcc accaccatca ccgccacctc tccgatagtg cgggattcaa ccccctttgt
     6361 ggtcgcctgt atcctctcct tttccttgtg agtaaaaaac gtatgtgcgt tacgcgccat
     6421 taccagtctc ctgatgctcc tccgcctcca aagccaccac cgccgccccc gccgaaatcg
     6481 ctgccacctc cgctgaagcc tcccccgccc gagctgcccc ctgttccaaa aaagccacct
     6541 ccgggccaga ccccgccgcc tctgtaacgt cctccggcag aaaaaaggag cggaagaagc
     6601 aatcctgcac caaagcccag cagggcaagc acagccgctg caccaagccc gagggagaac
     6661 cctgtgaaat gagctatgga gggcagggca atggcgcctg cggcacctcc cagaatcctc
     6721 gatatgcttc cgaggatgag gagggcaatt ccgccaaaga tgagaaaggt gaagagctgt
     6781 gaagatctct cccgccttgc ggggcgttgg gtcacgtcta ccttgaattc cccacgcgtt
     6841 gcatcgatta atgaggagac gcctgcaaga aagcccccgt caaaatcccc tctcttgaac
     6901 cttggcttca ccacgaggtc cacgattctc cctgccatta ggtccgtcat tctgccttcc
     6961 aatccccgcc ccacctcaat cctcatcttc cgttcctgct ttgcgacgat gaaaatgatg
     7021 ccgttgtcct tccccttctg gccgatcttc catgcatcgg caactctgat gctgaattcc
     7081 tccatcacct cgccctcaag ggagggaatg gtgagaatga cgatctgggt tgagtccgtt
     7141 tcttcgaaag agcgtaattc tttttccagc tcggtccttg ctggggggga tatgatattg
     7201 gcatagtcat tcacatagcc cctgagtttc ggaacatcaa gggcgtgtgc agaagaagaa
     7261 cagaggagca gaaggatgaa aagtgcgagg gaggcgcgta agcctcttcg cacagtcctc
     7321 tgcaaacgta tgacccttag ggattccact tactggaact ggacctttgg tgccttttca
     7381 gcccctgctt cagccttgaa cggttccttc agtttcagat ggagtaaaag actgttggtg
     7441 aggctgttcg ggaatatgcg cagggatgta ttgaatacct ccaccgcctt gttgtagcgt
     7501 gtgcgtgcca cgttgatcct attctccgta ccctcaagct ggtgctggag gtcctggaaa
     7561 ctctggtttg ccttgagatc aggatatttc tccacgacta ccatcagcct tgaaagggca
     7621 ctgctcatgg caccctgcgc ctcctggaac tgggcgaatg ccctggggtc atcgatgata
     7681 ttcttcgata cctgtacggt gcccaccttt gcccttgcct cggtgactgc agtgagggtt
     7741 tccctttcat gttttgcata cccttttacg acctccacaa gattcggaat aaggtcgttt
     7801 ctcctctgat acgacgcttc cacatcaccc catgctgcct ttaccgcctc ttcgtttgcc
     7861 tgcatggtat tgtaaccgca gccggaaaca gtaaaaagaa acaccaagga aaataggcag
     7921 agcaccggtc ttttcattat tccctccgta tttttttatc gtgattatat tgtaaaatat
     7981 ttcgagggaa aaattcaggc catgccagac gggaaaatga agatttctta attattgcag
     8041 gagcgatatt atggtattat tcatatctgt gaggatcagg gacttttttc tggcggtatg
     8101 tttgattttt gttttggcgc cccggctgga cgctgcctca tcgtattcat acgggaaggg
     8161 caatatggtg atcggagaag tccagaccta tgaggtcgag ggcgatgagt cactcattga
     8221 gatcgcgagg gactttgacg ttggatataa ccagattgag tccgcaaacc ccacccttga
     8281 cccctttgtt cccggagagg aggcactggt cactgtaccc acctcatgga ttttgcctga
     8341 gctgatttcc tatgacggta tagtgatcaa catctcggaa atgagactct actattttca
     8401 taaaatgaat cgttctcctg ttgtgaccac gtaccccatc ggaatcggca gcgagggcaa
     8461 tgatacgcct gtgggggtct ttaagataat ccagaagata gtggatcccc cttggtatcc
     8521 ccctccctct atccggaagg agaggcctga acttccgaaa gtgatccccc cgggtcctga
     8581 caaccccctc ggctcccatg ccctgcgact ctcattgggc actgtgctca tacatgggac
     8641 gaacaggcct tttgctgtgg gcagaaaggt cactcacgga tgtatcaggc tctatcctga
     8701 agatattccg caactcttta gtgtgattcc cgttggcatg aaagtgacca ttgtgcgaca
     8761 gccgataaag gtcggagtga gagacaaaaa agtgtatctg gaggtgcaca aggacgacta
     8821 ccgccaaaag gtggattatt ttaacgaggc ggtgaagctc ctcaggaaga agggcctgct
     8881 gaaggacgtg aatacagaga aaatctacca agcggtgcag gagaaacagg ggtttccggt
     8941 ggagattaac cagtagagaa aaaaaagggg tctaaatttt cttagacccc tgatcatttg
     9001 agacttcctg ttctatttca tttgctttaa ttcaaaggcc ttcacttctt ttgaagcggc
     9061 agcctcacac cttgcggcag cagcctcagc ccttgcagca gcagcctcag ccctctttgc
     9121 ggcagcctca gccttatcac aacactctgt tgcctttttc atagcatcct gagccaatgc
     9181 cttggcactt gctgcctctt tcttggcctg gtcaatatca gcagccattg accccacctt
     9241 ctgatccaaa gcgctcagct tggattccac ggcagagacc ctcgattcaa gcttgctgat
     9301 cctgtcgacc aatggatcta tctgctgttt cacataatcc tttgttacac acccaaaagc
     9361 aaagattgat aagaggctga ctaccaaaaa cattatcacc gtctttctca tatattcacc
     9421 tcctttccct aaacaatttc ttgcagtcgt gtcttgcaag ttcctattcc ttttagaaac
     9481 ttgcaaaccc ttttatgctc tttgtaaaat catacactga aagaaacagg aattcaacat
     9541 ctttttttgc tcttgtagcg agcacgtaaa ctgtccgcat ctgtagcaca taaactgtcc
     9601 gggtgataat ggatggcaag gaggtagcta tccatgaagc gttcggaggt actgcaggag
     9661 gtgcggaaga tgcggtttga ggaagcctat gggggatgga agcgtggtcg gctgacgcag
     9721 caggaggcgg cagggctgct tggggtgtgt gagcggacgt ttcggcgcta tct
//
LOCUS       DATKOU010000021         8945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_23880, whole genome shotgun sequence.
ACCESSION   DATKOU010000021 DATKOU010000000
VERSION     DATKOU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23880"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /gene="alaS"
                     /locus_tag="VMU21_00780"
     CDS             complement(<1..1686)
                     /gene="alaS"
                     /locus_tag="VMU21_00780"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUO76085.1"
                     /translation="MKSREIRTLFLEYFRGKGHEVVKSSPLIPKGDPTLLFTNAGMVQ
                     FKSVFLGEEQRTYSRATSCQKCVRAGGKHSDIENVGHTARHHTFFEMLGNFSFGDYFK
                     KDAISFAWELLTKQFSLPEEKLWVSVFENDDEAARLWADLTGVPEARIVRLGAKDNFW
                     QMGDTGPCGPCSEIIIDQGPEVGCKRKECAVGCDCDRFLEIWNLVFMQFNRDGKGTLT
                     PLPKPSIDTGMGLERLSAILQGKLNNFDTDIFSTIISAITRSTGTTYGKTPESDISIR
                     VIADHMRSIAFLLAEGLMPSNEGRGYVLRRILRRASRHARLLGVDKPLLHTLLASVSE
                     AMGDIYPELLEEEGRSSKLLMLEEERFTRTLEQGMRIMDDLISGAKESHEKVIPGKDL
                     FRLYDTYGFPLDLARDIATDNSLLIDEQGFHEEMQVQRERARASWVGEEEAVASIYRE
                     MLSEIGKTEFVGYITLESESVIKAILKDGKIIKEAGEGSEVEVFLDKTPFYGESGGQV
                     GDTGEIMGERFLASVIDTKNPLEGLHSHSIEIRKGTLRVWDKVRCRVDVEKRGA"
     gene            complement(1723..2082)
                     /locus_tag="VMU21_00785"
     CDS             complement(1723..2082)
                     /locus_tag="VMU21_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76086.1"
                     /translation="MKNVSLYLGALVVLMAWSFGGCASTARYSQDEIKDYPPAIQDNI
                     QKGEVAIGMTPQQVRYAWGAPSTINVLPPTENGKKKEEWLYSSGVFMKKRLLFIDDKL
                     VDIFPAPQQVNEIKKDE"
     gene            complement(2079..2672)
                     /locus_tag="VMU21_00790"
     CDS             complement(2079..2672)
                     /locus_tag="VMU21_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HUO76087.1"
                     /translation="MSILASAPRRERLAEDSPDKERRLPKRTPLEKSVTRQGEQAGLV
                     YAYKLLGYRGRSEKEIMRRLRIKGFEEPVIEKVIIRLKSSGLLDDRKLAFSLTRYAEE
                     SRTLSVAGTRKLLMERGIPRDLIHDALADMDETETARRLVEKRLTAWERRGLGSQPFH
                     LTPEITRKLYGILYRNGYPSEIIKRTLKQFICKEDME"
     gene            complement(2675..3820)
                     /locus_tag="VMU21_00795"
     CDS             complement(2675..3820)
                     /locus_tag="VMU21_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HUO76088.1"
                     /translation="MDINELLKKAVSQTASDVHIKVGSPPIVRVTGELMPMNDEERIS
                     QQDAMKIAFSVMSPGQREVFKQKNEIDLAYSVPGLGRFRCNIFIQRGTIGLVFRVISM
                     RIPSVEELNLPEVIKKIAMEERGLVLVTGTTGSGKSTTLATMIDYINMNRTCNIVTIE
                     DPIEYLHRDKKSIVNQREIGSDTESFGKALRSALRQDPDVILVGEMRDFETIQTGLVA
                     AETGHLVFSTLHTIDATETVNRVIAVFPPYQHKQVRMQLSSVIKGIISMRLMPKADGK
                     GRVPAVEVMIATATIKDCILDQDKTKLIPDVIAQSKIHYGMQTFDQSLFDLFKSGLIT
                     YEEALRRASSPDDFVLRVKGVRSTSDLTMDETIETRKTDVQIDRFAK"
     gene            complement(3820..4821)
                     /gene="recA"
                     /locus_tag="VMU21_00800"
     CDS             complement(3820..4821)
                     /gene="recA"
                     /locus_tag="VMU21_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUO76089.1"
                     /translation="MTNKDKTKALEMAISQIERSFGKGAIMKLGAGEVAEGIQAIPSG
                     SIALDIATGIGGYPRGRVVEVFGPESSGKTTLALHAIAEAQKTGGIAAFIDAEHALDI
                     NYAKKLGVKVEDLLVSQPDTGEQALEVAEALVRSGAVDVIVIDSVAALVPKAEIEGEM
                     GDSLPGLQARLMSQAMRKLTAAISKSLTTIIFINQIRMKIGVMFGNPETTTGGNALKF
                     YSSMRLDIRKIDNLKENQETVGGRVRVKVVKNKVAPPFKQAEFDIYFNEGISRVGELV
                     DVGVENGIIEKSGAWYSFSGNRIGQGRENVKEFLRNNPDISGSILQKITEHVKARTS"
     gene            complement(4818..5384)
                     /gene="thpR"
                     /locus_tag="VMU21_00805"
     CDS             complement(4818..5384)
                     /gene="thpR"
                     /locus_tag="VMU21_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HUO76090.1"
                     /translation="MRSFIAINLDDSLRSEIDRATTALKSGIWDVKWVSPANLHITLK
                     FLGEIPENTVTELKETLSSVVSHHESFALRFKGVGIFPDKRRPRVIWIDLLEAEKLIK
                     LQKEVENSLLTLGFKMEDRPFSPHLTIGRIRSSAGKESLLRAVETLKDKDFGNIGVNN
                     VSLMKSDLKPGGAQYTTIAEFSLNEEEQ"
     gene            complement(5381..5869)
                     /locus_tag="VMU21_00810"
     CDS             complement(5381..5869)
                     /locus_tag="VMU21_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HUO76091.1"
                     /translation="MDTSDRELIEKLHHHFKQSGLTLSIAESCTGGLISHLLTNLPGA
                     SSFVDSSVVCYSTESKVKLLGIRRSLIKNHGAVSEETAQAMAAAVRKKSGTDFSLATT
                     GNLGPDPMEDKKVGLVYIAVDRKRETVSKGMLFDGEREEIKHETARAALRFLSEVLEV
                     WA"
     gene            complement(5845..6303)
                     /locus_tag="VMU21_00815"
     CDS             complement(5845..6303)
                     /locus_tag="VMU21_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HUO76092.1"
                     /translation="MLISALLKNIATLGFIGYLPIAPGTFGTFAALLFFAFLKPSIPI
                     HVLLVILVTALGCIASSRAEKILNEKDSSHIVIDEFAGYALSVLLLPHTLSSLVASFL
                     LFRFFDILKPLPIRWIERTVPGGYGVMADDLLAGVYTNLVMRAWILLTGN"
     gene            complement(6410..6649)
                     /locus_tag="VMU21_00820"
     CDS             complement(6410..6649)
                     /locus_tag="VMU21_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76093.1"
                     /translation="MKTVKHEEMRKEYKREDLGKGIRGKYFEDYKKGTNLVLLSPDVA
                     AAFPDDASVNDALRSLMKIARQTTGLTKASRRTRE"
     gene            complement(6999..7298)
                     /locus_tag="VMU21_00825"
     CDS             complement(6999..7298)
                     /locus_tag="VMU21_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO76094.1"
                     /translation="MKYSFHPSAEKELTDAVDYYNAVQKGLGLEFAKEIHHTIQNIIS
                     FPNSWAPLSEHTRRCLSNRFPYGIIYQMAADEIYIIAVMQLSREPEYWKERKKKL"
     gene            complement(7295..7522)
                     /locus_tag="VMU21_00830"
     CDS             complement(7295..7522)
                     /locus_tag="VMU21_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HUO76095.1"
                     /translation="MIKTKDLFDEAISLPLEIRTKLIEKLLNSLNPSQKETDKLWAKE
                     AERRVEEIKSGKVKTIPGERVFKKIRDRCSS"
     gene            complement(7576..7866)
                     /locus_tag="VMU21_00835"
     CDS             complement(7576..7866)
                     /locus_tag="VMU21_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76096.1"
                     /translation="MKENQPSNFLKIVIWLTLGALVIGTLLNIYYGNVSHPKAFFVVL
                     VGFVLFAIGKLSVILKKRTISFGTNLMSENMANLYRLGYWLMIVGLLATFAP"
     gene            complement(8056..8637)
                     /locus_tag="VMU21_00840"
     CDS             complement(8056..8637)
                     /locus_tag="VMU21_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76097.1"
                     /translation="MKLTVLILLLSSLFLFISHISAEDVPTFTDDDLKKYGGKSSRGI
                     WNSDQSQIPRQTSHEGQRGDLAQEQANKSNRKLSDAEKNDIISQCTQVWHRYRNALSR
                     GDIEGALKEVSSSSIEAFRETLLYGRKPGHFGEIRAADIEENIAQFNMTVKDKLRPGD
                     DLAEGHSVGDYIEFEHYVNFIKEPTGAWKIDFY"
BASE COUNT         2056 a         2318 c         1972 g         2599 t
ORIGIN      
        1 tgcccctctt ttttcaacat cgaccctgca ccggaccttg tcccagacac ggagggttcc
       61 cttcctgatt tctatggaat gggaatgcag tccttccaga ggattcttcg tatcaatgac
      121 tgacgccagg aagcgttctc ccatgatctc ccccgtatca ccaacctggc cgccggattc
      181 accatagaag ggggttttgt ccagaaagac ctctacttcc gacccttccc ctgcttcctt
      241 tattatcttt ccatctttga ggatagcttt tatgaccgat tcagactcca gagttatgta
      301 cccaacaaat tcggtcttcc cgatttcaga gagcatctcc ctgtatatgg acgcaactgc
      361 ctcctcctct cccacccatg aggccctcgc cctttccctc tggacctgca tttcctcatg
      421 aaacccctgt tcatcaatga gcaggctgtt atctgtcgca atatccctgg cgagatccag
      481 gggaaaccca taggtgtcgt aaagcctgaa gagatctttc ccgggaatca ccttctcatg
      541 agattctttc gcaccggaaa taaggtcatc cattatcctc atgccctgtt caagggttct
      601 tgtaaaacgt tcctcctcca acataaggag tttggaggaa cgcccttctt cctccaagag
      661 ttcaggatag atgtcgccca ttgcctctga cacagaagcg aggagcgtgt ggagaagtgg
      721 tttatcaacg ccgaggagac gggcgtggcg ggatgctcgt ctcagaatcc tcctgaggac
      781 atagcctctc ccttcgttgg aaggcattaa gccttcagcg agaaggaagg cgatggacct
      841 catatggtct gctatgacgc gtatggagat atcgctctca ggggtcttgc cgtaggtggt
      901 gccggtggag cgggttatgg cggatatgat tgtggagaag atgtcagtgt caaaattatt
      961 caatttgccc tggagtattg cagaaagcct ttcaagaccc attcctgtat caatgctcgg
     1021 ttttgggagg ggtgtgagcg taccctttcc gtcacggtta aactgcatga atacaaggtt
     1081 ccatatctcg aggaacctgt cgcagtcaca tcccacggcg cattctttcc tcttgcagcc
     1141 cacttccggt ccctggtcga tgatgatctc ggagcatggc ccgcacgggc ctgtatcgcc
     1201 catctgccag aagttgtctt ttgctcccag tctcacgatc ctcgcttccg gaaccccggt
     1261 gaggtctgcc caaagtcttg ccgcctcgtc gtcattctcg aataccgata cccacagttt
     1321 ttcctcagga agggagaact gttttgtgag aagttcccac gcaaaggaga tggcgtcttt
     1381 cttgaagtag tctccaaagg agaagtttcc gagcatctcg aaaaaggtat ggtggcgtgc
     1441 ggtatgtccc acattttcga tatcgctgtg cttgcctcct gccctcacgc atttctggca
     1501 ggatgtagcc cttgaatacg ttctctgctc ctctccaagg aaaacggatt tgaattggac
     1561 catacctgca ttggtaaaga gcaaggtagg gtcaccctta gggataaggg gagaactctt
     1621 gaccacctca tgcccttttc cgcggaaata ctcaaggaaa agggttctta tttctctgct
     1681 tttcattatt gaccttttgt cccgggtacc ccctgtctat ttctattcat ccttcttgat
     1741 ttcattcacc tgctggggtg caggaaagat gtctacgagc ttatcgtcga taaacaggag
     1801 cctcttcttc atgaacactc ccgaggaata aagccattcc tctttctttt ttccgttctc
     1861 cgtgggggga agcacattga tagtggaagg ggcaccccat gcataacgca cctgctgggg
     1921 ggtcattcct atggctacct cccccttctg gatattgtcc tggatcgcgg gcggatagtc
     1981 cttgatttcg tcctgggaat accttgctgt tgacgcacaa cctccaaaag accatgccat
     2041 gagcaccacg agtgctccca gataaagaga aacattcttc attccatatc ctccttgcag
     2101 ataaattgtt ttagggtcct ttttattatt tcagacggat atccgttccg ataaaggatg
     2161 ccgtacagtt tccttgtgat ctcaggagtt aggtgaaaag gttgactgcc caacccacgc
     2221 ctctcccagg cggtgagtct cttctccaca agtctcctgg cagtttctgt ttcgtccata
     2281 tccgcgagtg catcatggat gagatccctg ggaatccccc gctccatgag aagctttctc
     2341 gtacctgcga cacttaacgt tctggattcc tcagcgtatc gtgtgaggga aaaggcaagc
     2401 ttcctgtcgt ccagcaatcc ggaggactta aggcggatga tgaccttctc aataaccggt
     2461 tcctcaaatc cctttattct gagccgcctc attatctcct tttcgctcct ccccctgtac
     2521 ccgagaagct tatatgcata cacaagccct gcctgttctc cttggcgggt aaccgacttc
     2581 tccagaggtg tcctcttggg taaccgacgc tctttatcag gagaatcctc tgccagtcgc
     2641 tcgcgtcgcg gggcagatgc aaggatgctc actctcattt tgcgaatctg tcgatctgca
     2701 cgtcggtctt tcttgtctct atggtttcat ccattgtgag gtcgctggtg gaacggacgc
     2761 ccttcaccct gagaacgaaa tcatcaggac tggatgccct ccgcaaagcc tcttcatagg
     2821 tgatcagtcc tgatttaaag aggtcgaaaa gggactggtc gaatgtctgc attccgtaat
     2881 ggattttgct ctgtgcgatg acatcaggga taagctttgt tttatcctgg tcaagaatac
     2941 aatctttaat ggtggcagtg gcaatcatca cctcgactgc aggcaccctc cctttgccgt
     3001 ccgctttggg catgagtctc atggagataa tccctttgat tacagaggaa agctgcatcc
     3061 tcacctgttt atgctggtaa gggggaaaca ctgcaataac cctgttcacc gtctctgttg
     3121 catctatcgt atgcagggta ctgaagacga gatgtcctgt ctcagcggca acgaggccgg
     3181 tctggatggt ctcaaagtcc ctcatttcgc ctacgaggat cacgtccggg tcctgtcgca
     3241 gtgctgagcg caacgccttg ccaaaggatt ccgtgtcgct gccgatctcc ctctgattca
     3301 ctatgctctt cttatccctg tggagatact ctatggggtc ttcaatggtg acaatgttac
     3361 aggtcctgtt catgttgatg taatcgatca ttgtggcgag cgtggtggat ttcccgctcc
     3421 ctgttgtccc cgtgaccagg acaaggcccc tctcttccat cgcaatcttc ttgatcacct
     3481 cgggaaggtt gagctcttct acggacggaa ttctcatgga aataaccctg aatacgagac
     3541 cgatggttcc cctctggatg aagatgttac atctgaatct gcccagtccg gggacgctat
     3601 aggcaaggtc aatttcgttt ttctgcttga aaacttcacg ttgcccgggg ctcattacgg
     3661 aaaatgcaat cttcatcgca tcctgctgcg agatcctttc ctcgtcattc atgggcatca
     3721 gttcgccggt gacgcggaca atgggaggag agcccacctt gatgtgaacg tccgatgcag
     3781 tctgagaaac agccttcttg aggagttcat tgatatccat tatgatgtcc ttgctttcac
     3841 gtgttctgta attttctgga gaatggatcc agaaatatca gggttattcc taagaaactc
     3901 ttttacattc tctcttccct ggccgattct gtttccgcta aagctgtacc acgcgcctga
     3961 tttctctatg atcccgtttt cgacacccac atccacaagc tctcccaccc gcgagattcc
     4021 ttcgttaaag tatatatcaa actctgcctg tttgaaaggc ggggcaacct tatttttcac
     4081 caccttcacc cgtaccctac cccctacggt ctcctgattt tcctttaagt tatcgatttt
     4141 tcttatatcg agcctcatgg aggagtagaa cttcaatgcg tttccccccg tggtcgtttc
     4201 gggattgccg aacatcacac ctatcttcat cctgatctgg ttgataaaaa taatcgttgt
     4261 cagggatttt gagatggctg cagtgagctt cctcatggcc tgactcatga gccttgcctg
     4321 gagcccggga agtgaatccc ccatttctcc ctctatctct gccttcggga caagggctgc
     4381 aacggaatcg attacgatga catccactgc tccgctcctt accagggcct ctgccacctc
     4441 cagcgcctgt tcaccagtat caggttggga taccaggagg tcttccacct ttacgcccag
     4501 tttcttcgcg taattaatgt caagggcatg ctctgcatca atgaatgccg caatgccacc
     4561 tgtcttttgg gcctctgcaa tagcatggag agcaagggtc gtctttcctg aagactcagg
     4621 gccaaatacc tcgaccactc tgccccgggg gtatccgcca attcccgtgg cgatgtccag
     4681 ggctatcgag cctgagggaa tagcctggat gccttcggcg acctctccgg cgcccaactt
     4741 cattatggca cctttgccaa agctccgttc aatctgcgaa atggccattt ccagggcctt
     4801 tgttttgtct ttattggtca ttgctcctcc tcatttaggg agaactcagc tattgtcgta
     4861 tactgagcac ctcctggttt taaatcgctt ttcattaacg agacgttatt gactcctata
     4921 ttaccaaaat ctttatcttt tagtgtctcc actgccctca ggagggattc tttgcctgca
     4981 gacgacctta tcctccctat ggtaaggtgg ggtgaaaacg gtctatcttc cattttaaaa
     5041 ccaagagtta acaaactatt ctcaacttct ttttgaagtt ttatgagctt ctctgcttca
     5101 agaaggtcga tccatattac ccttggcctt ctcttgtcag ggaatattcc cactcccttg
     5161 aatcttaaag caaaagattc gtgatgagaa acaacggatg aaagagtctc cttcagctct
     5221 gttacggtat tctcgggtat ttcgcccagg aacttaaggg taatatggag gtttgcaggg
     5281 gaaacccact ttacatccca aatccctgat ttcagggcgg ttgtcgctct gtcaatctcg
     5341 cttctcaggg agtcatcaag gtttatggca atgaagctcc tcatgcccag acttcgagca
     5401 cctcgctaag aaaccggagt gctgcccttg ctgtctcatg ctttatttct tccctttccc
     5461 catcaaagag catgcctttt gaaacagttt ccctcttccg gtccactgca atgtacacaa
     5521 ggcccacttt cttgtcctcc atcgggtctg gaccgagatt accagtggtt gccagggaaa
     5581 aatcggtgcc gcttttcttc ctcaccgcag cagccattgc ctgagcagtt tcttcactca
     5641 ctgccccatg gttcttgatc aatgacctcc tgattcccag gagcttcact ttggactctg
     5701 tggaatagca caccacagaa gaatcaacga aagaagatgc tccggggagg tttgtcagca
     5761 aatggctgat gaggccacct gtgcaggact ctgcaatgct cagcgttaac cctgactgtt
     5821 tgaaatggtg gtgaagtttt tctatcagtt ccctgtcaga agtatccatg cacgcatcac
     5881 taggtttgtg taaactccgg cgagcaagtc atcagccatg acgccatacc cacctgggac
     5941 agttctctct atccatcgta tgggaagcgg ctttagtata tcgaaaaacc tgaataaaag
     6001 aaaggacgca acgagagagg atagtgtgtg aggaagaagc aaaactgaaa gggcgtaacc
     6061 tgcaaattcg tcgatgacga tgtggctgct gtccttctcg ttcaatatct tttctgccct
     6121 ggatgacgca atacacccca gtgcagtgac aaggattacc agcaaaacgt ggataggaat
     6181 ggatggtttt aggaaggcaa agaagagaag ggcggcaaaa gtgccaaagg tccctggcgc
     6241 tatgggtaga taccctatga aaccgagagt ggcaatgttc ttgaggagcg ctgaaatcaa
     6301 cattacgctt agattatcac aaaaatgctt tggtgtcctt actatttaga cataacgaca
     6361 gagctaagag gcgcggggta agtaggccag aactgaaaaa gaaaagcgac tattcccgcg
     6421 tccttctcga tgcctttgtt agaccggttg tttgccgtgc tattttcatc aagcttcgca
     6481 gggcatcatt gaccgaggca tcatcgggga aagccgctgc cacatcagga ctgagaagaa
     6541 caagattggt tcctttctta tagtcctcga aatatttgcc tcttattcct ttccctaaat
     6601 cttctcgttt atattctttt ctcatttctt cgtgtttaac cgttttcata tatttccctc
     6661 tcctgtcttg ttgcgcgcct cgcactcatt attcttgttt ttccacgtcg ctcagtatga
     6721 acgacaacca ttaactgatt ggctctcgaa aggccaaatg tcaggaatct atgctcaccg
     6781 attgaatggt cttggtcatt gaaggttatt gccagtggat ctccaaagac tgttgacgct
     6841 tcatggaaag atatgccgtg tttcttgagg ttggcgtctg acttgcttgg gtcccattcg
     6901 aattccacgt cttattttat cattattcct aatattattt tgtgggctaa cgaccgcaat
     6961 atgcggcgga gagaaacgga gtccgcatga ttgatttgtt ataacttctt ttttctctcc
     7021 ttccaatatt caggctctct gctcagttgc atcaccgcta tgatatatat ttcgtcagcc
     7081 gccatctggt aaataatccc atagggaaaa cggttgctca gacaccttct ggtgtgttct
     7141 gaaagcggcg cccatgaatt tggaaatgat atgatgtttt gaatggtatg atggatttct
     7201 tttgcaaatt ccagcccgag acctttttga actgcgttgt aataatcaac agcatcggtc
     7261 agttcctttt cagcagaggg atgaaaagaa tatttcaaga gctgcaccgg tcacgaattt
     7321 tcttaaatac tctttctccg ggaattgtct taacttttcc gctctttatt tcttctaccc
     7381 tcctctctgc ttcctttgcc cataatttgt cagtttcttt ttgcgatgga ttcaagctgt
     7441 tcagaagctt ctcaataagt tttgttctta tttctaaagg tagcgaaatt gcttcatcga
     7501 agaggtcttt ggtctttatc attgctgcct cctaaccatt gaatttattc tattatatca
     7561 tttcctccgg ctacgttacg gcgcgaatgt agcaagcagg cctacaatca tcagccaata
     7621 tccgaggcga tagagatttg ccatgttttc actcattaaa tttgtgccga aactgatcgt
     7681 ccttttcttc agaattaccg aaagcttgcc tatagcgaaa aggacaaagc cgacgagcac
     7741 tacgaagaaa gctttgggat gagaaacatt gccgtaatag atatttaata aagtgccgat
     7801 caccagcgca ccgagcgtta gccaaatgac aattttcaga aaattactcg gttgattttc
     7861 tttcatgaga ggcacactat gcattgcgat cacccatatc ctttttcttg acttttacgt
     7921 tataacgata cggctgaccc gcagagcaat cgccgaaagc ttaaagccac aaagctgtcc
     7981 acagtcggat cgaaccgctt gttatggtcc gccttttcct ttctaggcaa gagggctcta
     8041 atgatattaa ttctgtcagt aaaaatcaat tttccaggct ccagtaggtt ccttaataaa
     8101 atttacgtaa tgctcaaatt caatataatc accgacggaa tgaccctcgg cgaggtcatc
     8161 gccagggcgc aacttatctt taacggtcat attaaactgg gcaatgtttt cctcaatatc
     8221 cgcggctctg atttccccaa aatgtccagg ctttcttcca tataggagag tttctctaaa
     8281 agcttctatt gagctgctcg aaacttcctt taatgctcct tctatgtctc ccctggaaag
     8341 tgcattccta taccgatgcc aaacttgcgt acactgtgaa ataatgtcgt tcttctctgc
     8401 atcgctgagt tttctatttg atttgttcgc ctgctcttga gccaaatcac cgcgctgccc
     8461 ctcatgactt gtttgccgag gaatctgaga ttggtcactg ttccagatgc cccgcgatga
     8521 ctttccccca tattttttca agtcgtcgtc tgtgaaagtc ggtacgtcct ctgcggatat
     8581 atgagaaata aacaaaaaca gggaactcaa tagtaatata agaactgtta gtttcattgc
     8641 cctcctcctt caaggcaatt aatctttaat aatcgataag aatatctgca cgaccccttc
     8701 agcttcattt ttcattattt attgttgcca taacgatgcc gatgaggtgc ccccgaaggg
     8761 agacagctcc attggtttgt tcggcgtctt tattataccg aacacccata tagcgggcac
     8821 ctgtcaagcg aaaaagatat cttatcgaga agtttttgcc ttcccctttt tatgagctgc
     8881 cgacgatccc accaatgccg ttttttcatt gggcttcggc tgcagtcttc actgtgccag
     8941 acacc
//
LOCUS       DATKOU010000022         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5413966, whole genome shotgun sequence.
ACCESSION   DATKOU010000022 DATKOU010000000
VERSION     DATKOU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5413966"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /gene="argS"
                     /locus_tag="VMU21_00845"
     CDS             complement(<1..1498)
                     /gene="argS"
                     /locus_tag="VMU21_00845"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HUO76098.1"
                     /translation="MEKSIRELIEKAIKNMGVESYPVELEIPREERFGDLATPAAMGL
                     SKLLKRPPRQIAEEIVRHIGDTDVCERVDIAGPGFINFSFSRTFLYSQLKSLLSFTKD
                     FVRKDMGKGRKVQVEFVSANPTGPLHLGHGRGAAVGAALSNLLEAAGFRVEREYYVND
                     AGRQVKVLGHSVFAKYQQLLGIEYPFPEDGYKGGYVEEIAKKVVSLVGSKYSETSFQD
                     ASDFFVDFSYRQMLREIEEDLGAFGITFDTWQSERELYTGNEVDNAIDDLRRRRHLYE
                     ENGALWFRATTFGDDKDRVIIKGDGEFTYFASDIAYHRKKIEKGFDEIVDIWGADHHG
                     YITRLKAVIEALGYPPEHLRVLLVQMVTLLRAGNPVQMSKRAGEFVTLREVMEEVGSD
                     TTKFIFLTRRPDSHLEFDLEVAKEQSAENPVFYVQYANARINSIFVHAREKGMEGKDF
                     TEADLSLLSAPEELRIIKKLLTYLMTFEGAAISREPHRITFFLQELTGL"
     gene            1683..>2255
                     /locus_tag="VMU21_00850"
     CDS             1683..>2255
                     /locus_tag="VMU21_00850"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HUO76099.1"
                     /translation="MMYKILKKEILSDTVKLMDVYAPHVARNARAGQFVILIIDEHGE
                     RIPLTIADYNKGTGTITIIFQELGKSTMQLGRLKPGDAIASFAGPLGHPTEIENYGTV
                     VCMGGGVGIAPIYPIARSLKEAGNSVISIIGARNKELLFWEDKMRTVSDELIVCTDDG
                     SHGRKALVTEPLKELFERKDKKVARVWAIGP"
BASE COUNT          563 a          602 c          515 g          575 t
ORIGIN      
        1 agaggcctgt gagctcctgg agaaaaaagg taatcctgtg gggctcacgg gaaattgccg
       61 cgccctcaaa ggtcatgagg taggtgagca gcttcttgat tatcctgagc tcctcaggcg
      121 cagacaggag gctcaggtca gcttcagtaa agtccttccc ctccattccc ttttcccttg
      181 catgcacgaa aatactgttt attctcgcgt tggcatactg cacgtagaat acagggttct
      241 ccgcggactg ctcctttgcc acttcaaggt caaactccag atggctgtca ggccttcttg
      301 tgagaaagat gaactttgtg gtgtcgctgc ccacctcttc cattacctcc cgaagcgtca
      361 caaattcccc tgccctcttg gacatctgga cagggtttcc tgccctcagg agggtcacca
      421 tctggacaag cagcaccctg aggtgttccg gcggataccc cagcgcctcg atgaccgcct
      481 tcaacctcgt aatatacccg tggtgatcag ctccccatat gtctacaatc tcatcaaaac
      541 ccttctctat cttcttcctg tggtacgcga tatcagaggc aaaataggta aactctccgt
      601 cgcccttgat gatcacgcgg tccttgtcat caccaaaggt agtggccctg aaccataacg
      661 ccccattctc ctcgtagaga tgcctcctgc gcctgagatc gtcaatggca ttgtccacct
      721 cattcccggt ataaagttct ctttcactct gccatgtgtc aaaggtaata ccaaaagccc
      781 caaggtcttc ttcaatttcc ctgagcatct gacggtaaga gaaatccaca aaaaagtctg
      841 acgcgtcctg aaaactcgtt tcagaatatt tgcttcccac caatgagaca actttcttcg
      901 cgatttcctc gacatagcca cctttatatc catcttcagg gaatggatac tcgatgccaa
      961 ggagttgctg atactttgcg aatactgagt gccccagtac cttcacctgc cttcccgcat
     1021 cgttgacgta atattcccgc tccaccctaa atcccgcagc ttccaggagg ttggaaaggg
     1081 cagcacccac tgcagcgcct cttccgtgtc caagatgaag agggcctgtg gggttcgcac
     1141 tcacgaactc gacctgcacc ttcctccctt tgcccatatc cttcctcaca aaatccttcg
     1201 taaaggaaag aagggactta agctgggaat agagaaaggt tcgcgaaaag gagaaattta
     1261 taaaaccagg gcctgcaata tctacccgtt cacacacatc tgtatccccg atatggcgca
     1321 caatttcctc tgcgatctgt ctcggcggcc tcttgagaag tttggataac cccatggcag
     1381 caggtgtggc aagatctccg aaccgttctt cccgggggat ttcgagctcc accgggtacg
     1441 attccactcc catgttcttt attgcctttt ctataagttc ccgtatgctc ttttccatag
     1501 ggataaagtt taaacttcaa gcacatttaa gtcaatgaca gcgatattcg gaccagtggg
     1561 aaaaatacat cttgggtttg tttttgacct gtttttcagt gtgccgtcgc aaatggactc
     1621 tccatgtttg attatggtaa aataaaaaaa gattcgttcc aattatccgc ataagggagg
     1681 gaatgatgta taagattctc aagaaagaga tcctgagtga cactgttaaa ctcatggatg
     1741 tgtatgctcc gcatgttgcc agaaatgcga gagcgggcca atttgtgatt ttgatcatcg
     1801 atgaacatgg cgaaaggatc cctctcacta ttgctgacta caacaaagga acggggacca
     1861 taacgattat ctttcaggaa ctggggaaat ccacgatgca gcttggaagg ctcaaacccg
     1921 gagacgcgat agcatccttc gctggtcccc tgggacatcc tacagagatt gaaaactacg
     1981 ggaccgtggt atgtatgggc ggtggtgtgg ggattgcccc tatttatcct atagcgaggt
     2041 ccctaaaaga ggctggcaat tctgtcatat ccattatagg cgcccgaaac aaggaattgc
     2101 tcttctggga agacaagatg agaaccgtgt ctgacgaact catcgtctgc actgatgacg
     2161 gttctcatgg gagaaaagcg cttgtcactg aacctctcaa agaactgttc gagcgcaagg
     2221 acaaaaaggt cgcccgtgtt tgggccatcg gaccc
//
LOCUS       DATKOU010000023         3323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3105992, whole genome shotgun sequence.
ACCESSION   DATKOU010000023 DATKOU010000000
VERSION     DATKOU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105992"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VMU21_00855"
     CDS             <1..276
                     /locus_tag="VMU21_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76100.1"
                     /translation="KTKYNNFLVQPFGNYNFGKTGTYLCSSPLITANWRTGDWVVPLG
                     GGIGQITKVFGNQPVDIQLQAFYNVVHPDNLGPEWSMRFQVKLMFPK"
     gene            483..1211
                     /locus_tag="VMU21_00860"
     CDS             483..1211
                     /locus_tag="VMU21_00860"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HUO76101.1"
                     /translation="MKKKILKSFVVSVGMAVLFAGCATANKAPKYSGFLSDYSKLAPD
                     PDGSGAEEYINPAADKKKYHKILLDKIMVSLKDNEEYKAVDPDALKTLTDYLHDAIVK
                     EVGDAYPIVNEPGPDVERVRIAITDIAPTKTAMTVIVLLAPYGSVADLASGAVSKGGV
                     GSAPYLGHAGVEAEGLDSETLQVVFSYVDQRFGKKYDPKNPAGAYFGAYKEWAYVKDF
                     FDYWAKKFRQRLDELHGIKPIQKG"
     gene            1507..1632
                     /locus_tag="VMU21_00865"
     CDS             1507..1632
                     /locus_tag="VMU21_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76102.1"
                     /translation="MRTTRAQYLIPLSPVGMLAITGRGQAQDALPPLVKQMLSGA"
     gene            1643..2098
                     /locus_tag="VMU21_00870"
     CDS             1643..2098
                     /locus_tag="VMU21_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HUO76103.1"
                     /translation="MPLRILIACVLVVVTVTIHAVGFSLLLRALMRSHALTTTGFWQA
                     TWLVIGLTSWLLLIHLAEIAVWGQCYFWLGCFPDVESAHYFSGVTYTTVGYGDLVLPK
                     QWRVLAPIEALTGLLMCGLSTGLFFAVTSRRISYWMKERTSLNQMKEGL"
     gene            2152..2784
                     /locus_tag="VMU21_00875"
     CDS             2152..2784
                     /locus_tag="VMU21_00875"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76104.1"
                     /translation="MIRRGEITLQEEAVKKSAMVIVLFLCMIIPISAFAGAPLDSVKV
                     NVDRVLDVLRDPSLKPESAKKLKKDKIRAIAKKMFDFAELSKRTLAQNWSRFSPDQQK
                     EFINLYHSLLEDAYVDKIMAYTDEKILFIKDIPLTEKTAEVQSTVIKKTDEIPINYRV
                     ILKDGVWMVYDVVIEGVSLINNYRSQFREILANKPPESLLETLRKKVGKA"
     gene            2988..3221
                     /locus_tag="VMU21_00880"
     CDS             2988..3221
                     /locus_tag="VMU21_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76105.1"
                     /translation="MKQKRSLELLKRPLIILILALLVAGGLFFWYEYRPSTVRARCST
                     EAEKMAGKDEFVYEIIYRHCLRSHGIEYSEKKE"
BASE COUNT          901 a          727 c          900 g          795 t
ORIGIN      
        1 aaaacaaagt ataacaattt cctggtccaa ccgtttggga attacaactt tgggaagacc
       61 ggtacatacc tttgcagttc accgcttata accgctaact ggaggacagg ggattgggtg
      121 gttccgctgg gtggcggcat agggcaaatc actaaggttt ttggaaatca accagtagac
      181 attcaactgc aggcatttta taacgtcgtc catccggata atttgggtcc tgaatggtcg
      241 atgcgcttcc aggtaaagct catgtttcca aaatgagaga gccggtgaag ttctcgaact
      301 tgtgcgcccg gaggccgact cacacaaaat ccgtctggcg ctcgaaaccg gttcctcgca
      361 tccagccgtg cgcggaaatg gtgtagacgt gggaggaaag tcactgcttc aggaaatgga
      421 gatcgaaaaa gaacatgtga tgaaataatg ataaggctgg gacaaaataa aaggaggatg
      481 agatgaaaaa aaagattctg aaaagctttg tagtaagcgt gggaatggca gtgctctttg
      541 cagggtgcgc aacagcgaat aaggctccca agtattccgg cttcctgagc gattattcaa
      601 agcttgcacc ggatccggat ggttcgggag cagaggaata tataaatccg gctgcagaca
      661 agaaaaagta tcacaagata cttcttgaca aaataatggt gtccctgaaa gacaatgaag
      721 aatacaaggc tgttgatccg gatgccctta agacgttgac tgattatctc catgacgcca
      781 tcgtgaagga ggtgggagat gcctatccga tagtgaatga acccggaccc gacgtagaac
      841 gagtgaggat cgccattact gacattgccc cgaccaagac agccatgacc gtgatcgtgc
      901 tgttagctcc ttacggcagt gtcgctgatc ttgcatcagg ggctgtttcg aagggcggcg
      961 taggcagcgc tccctatctg ggtcatgccg gagtagaggc cgaagggctt gacagtgaga
     1021 cgctccaggt cgttttttct tatgtggacc agcggtttgg aaagaaatat gaccctaaga
     1081 accctgccgg cgcgtacttc ggggcttata aagagtgggc ttatgtgaag gatttttttg
     1141 actactgggc taagaagttc cgtcagcgtt tggacgaact ccacggaata aagcctattc
     1201 agaagggata atattttgtt gaagagatgc caatagacct ggcacttgca gggactattg
     1261 ttattatttg gtgctgaaac ccgggtaaga tctgaaatgg atgggcggaa gcattccgcc
     1321 catcttaaga taagaaatta ttttttgagg cactccattt tattactgtg gtaaaatttg
     1381 agggaaagtg aaagaagaga atttggatca tcgcatgaaa gttgaaatcg gcatcagtgg
     1441 ggattcaggc tttcaggaag aagtaacgcg tgaagtgaaa caatcaaaca aagtgaggac
     1501 acaaccatga gaaccacacg cgcccaatat ctcatccccc tctcgcccgt tggcatgctg
     1561 gccatcaccg gaagggggca ggcgcaagac gccctgcccc ctctcgtgaa gcaaatgctc
     1621 agcggcgcgt gattatccgc ctatgccctt aagaatcctt atcgcttgtg ttctggtggt
     1681 cgtcaccgtg accatccatg ccgtcgggtt ctccctgctg ctcagggcct tgatgagatc
     1741 gcacgccctc accacgacgg gcttctggca agccacctgg ctggtgatcg gcttgacgtc
     1801 ctggctgctc ctgatccacc tggccgagat tgcggtctgg gggcagtgct atttctggct
     1861 gggctgcttt cccgatgtcg agtctgcgca ctatttttcc ggagtcacct acacgaccgt
     1921 tgggtacggc gatctggtgt tgccgaagca gtggcgcgtg cttgcaccaa tcgaggcatt
     1981 gacgggcctt ctcatgtgcg ggctctcgac cggtctgttc ttcgcggtga cgagccggcg
     2041 gattagctac tggatgaaag agagaacatc gttaaaccaa atgaaggaag gactatgaga
     2101 caaggcagga gtattagctg tgcagaacaa ttaagaggtc attgaaaaaa gatgataaga
     2161 aggggcgaaa taacccttca ggaggaagca gtgaagaagt ccgccatggt aattgtgctc
     2221 tttctctgca tgatcattcc gatctcggcg tttgcaggcg cgccgctcga ctctgtgaag
     2281 gttaatgtgg acagggtgct ggatgtgctg cgtgatccat ccctcaagcc cgaatcagcc
     2341 aaaaagctta aaaaagacaa aatccgcgcg atcgcgaaaa agatgtttga cttcgccgag
     2401 ctctcaaaga ggactttagc ccagaactgg tccaggttct ctcctgacca acagaaggag
     2461 ttcatcaatc tctaccactc ccttcttgaa gatgcgtatg tcgataaaat catggcctac
     2521 actgacgaaa agatactctt tatcaaggat attcccctga ccgaaaaaac tgctgaggtg
     2581 cagagcacgg tcatcaaaaa gactgatgag atccccatca actacagagt gatcctgaag
     2641 gatggcgtct ggatggtcta cgatgtcgtg atagaaggag ttagcctcat aaataactat
     2701 cggagtcagt tcagggagat cctcgcgaac aaacctcctg aatcattgct cgaaacccta
     2761 aggaagaagg tcggaaaggc ctgatgcatg cataaaacct cctatatctg tgtgaaacac
     2821 tctgcgctga caagacgatt ggagatgtca ggtacgggaa ggagtcgata caaggccctg
     2881 gggtaactca acgtctttta ttgttttagc gacgattttg ttatgctttg caggtagagc
     2941 aacactctac agaggaagat aaccataatc ttgtcgaggt gaatgtaatg aaacagaaaa
     3001 ggtcattaga gcttttaaaa aggccgctta taattctcat tcttgccttg ttggttgcag
     3061 gggggttgtt cttctggtat gagtaccgac cgagcacggt gagagcgaga tgttcgaccg
     3121 aagcggaaaa gatggcgggt aaggatgagt ttgtctatga aattatttat cgacattgtt
     3181 taagaagcca tggtatagag tactctgaaa agaaagagta aatagactat gttttgatca
     3241 atatgctgtt gcgcgctctc gaccggtctg ttcttcgcgc tggtgagccg gcggattagc
     3301 tactggatga aagagagaac atc
//
LOCUS       DATKOU010000024         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5418073, whole genome shotgun sequence.
ACCESSION   DATKOU010000024 DATKOU010000000
VERSION     DATKOU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5418073"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VMU21_00885"
     CDS             complement(<1..428)
                     /locus_tag="VMU21_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUO76106.1"
                     /translation="MYRIVLAGCVILALVTGCKKVEKASQPEKPQGNPLVIGLIPEQD
                     IFSQVERYRPLADYVSRKVGRKIELKVLTRYGNIVNNFVSSGMDGAFFGSFTYTLAHA
                     KLGVEALARPVDLDGASTYCGLLFVRKDSGIISIKEMRGKR"
     gene            616..1683
                     /gene="rlmN"
                     /locus_tag="VMU21_00890"
     CDS             616..1683
                     /gene="rlmN"
                     /locus_tag="VMU21_00890"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HUO76107.1"
                     /translation="MHTKTNLKALTPQQAASFFHDAGLPSFRARQLLHWMYEKYAQSI
                     EGITEFSQTLRKRLSVQSYISNLTPLKRETSEDGTEKFLFSLEDGESIESVLIPDKDR
                     LTLCISSQVGCAMGCLFCLTGTLGFKRNLKAYEIVDQCISVSRLIAPEKPTNIVFMGM
                     GEPLSNFDEVVDSLWRMTDLMKISKRRITLSTAGIVPKILELGSAGQSAGQTVFSARM
                     RAPRVNLAISLNATTDKVRDRLMPVNKTYPIRSLLDACRRFPLEPRRRITFEYVMLSG
                     INDSAADAKRLITLLRGIPCKVNLIPFNPYRGCEFGRPSDETVLTFQKILLDAHMTAL
                     IRKSKGQDILAACGQLKAGYG"
     gene            complement(1680..2048)
                     /locus_tag="VMU21_00895"
     CDS             complement(1680..2048)
                     /locus_tag="VMU21_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO76108.1"
                     /translation="MPSILVVEDSPTMRQLITFAMKRIANAKVIEATDGVDALKKLSS
                     EKIDLILADINMPVMDGLKLVSLVKNNQSYKDIPVIIITTEGAKEDKERALAIGANAY
                     LAKPIQTQELIKLVNSFIGS"
     gene            complement(2057..2878)
                     /locus_tag="VMU21_00900"
     CDS             complement(2057..2878)
                     /locus_tag="VMU21_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUO76109.1"
                     /translation="MEKDPVITRRVDEFLEIFKKGEEFTKDLLRENEKLRFRVAQLEE
                     ATLSSAAEEKIRLYEERIKKLDEEINSVKERYLKVEEENKDFASKYVEVEEINNALAN
                     LYVASYQLHSTLDFDEVLRIIIEIVINLIGAEAFAILLIDEKTNDIVPVSADGIPLES
                     VPKTRIGEGIIGSVAKEGESYFAADITSGGDVAQGLPIVCIALKIKEHVIGVIAIYSL
                     LTQKEGFTNVDYELFNLLAGHAATAIFSAKLYTQSERKLTTIQSFLELLKEKPKR"
     gene            complement(2857..>3609)
                     /locus_tag="VMU21_00905"
     CDS             complement(2857..>3609)
                     /locus_tag="VMU21_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HUO76110.1"
                     /translation="IQGDLLGKIKGIRDLEMGKLSISRELLLHHSPNEARRDEVYEGS
                     EESSEEHGVVAIGASTGGPTALQIVLTRLPWNLPAGIVVSQHMPRGFTKPFTERLAKL
                     TRIKVREAADGDIVEPGKALVCPGGHNMILKKKHQTIRVILKEATGRDKYVPSVDLMM
                     SSAAEHFGPMTVGVVLTGMGNDGKAGVVEIKKKGGYTIAEAESTAVIFGMPNEAIKTG
                     AVDRVLPLHKIPGEIIMTVAGKRDRKWKKTQS"
BASE COUNT          805 a          990 c          864 g          950 t
ORIGIN      
        1 cgcttgcccc tcatctcttt tatggagatg ataccgctgt ctttcctcac aaagagcagt
       61 ccgcaatatg tcgatgcccc gtcaagatct accggtctcg caagggcctc cacacccagt
      121 tttgcgtgtg caagggtata ggtaaaactt ccaaagaatg ccccatccat gccggatgag
      181 acaaaattgt tcacgatatt tccgtatcgg gtgagcactt tgagctctat ttttcgcccg
      241 accttcctgg aaacataatc cgcgaggggt ctgtacctct ccacctggct gaagatgtcc
      301 tgctcaggga tcagccctat cacgagaggg tttccttggg gtttctcagg ctgagaggcc
      361 ttctcaacct tcttgcagcc tgtgacaagg gcaaggataa cacatcccgc aagaactatt
      421 ctgtacattg gcctatttta tatattcctg aggggataat tcaagggaga aaagagatat
      481 agggatgcta attttcatat atgatgtggg gaagatctct ctcgcgggtc agcgtccggc
      541 agaccgcagg aacagaccac gacaaaggtg ctataatgaa caattcccga agaacaatgt
      601 gaggcccagg atcacatgca tacaaagaca aacctcaaag cccttacccc tcaacaagca
      661 gcgtcctttt tccatgacgc gggtctgccc tcattcaggg cgcgccaact cctccactgg
      721 atgtacgaaa aatatgccca atccattgaa ggcatcaccg agttttctca aacactccgg
      781 aaaagactgt cagtgcagtc ctacataagc aacctcaccc ccctgaagcg ggagacgtcc
      841 gaagacggga ccgaaaaatt cctcttctcg cttgaagacg gagagagcat cgagagcgtt
      901 ctcatccctg ataaagacag gttgaccctc tgcatctcct cgcaggtcgg gtgtgccatg
      961 gggtgcctgt tctgccttac aggaacactg gggtttaaga ggaacctgaa ggcatacgag
     1021 attgttgacc agtgtatctc ggtgagtcga cttatcgcac cggagaaacc taccaacatc
     1081 gtattcatgg gcatgggcga gcctttgtcg aattttgatg aagtggtcga ttcattatgg
     1141 aggatgactg acctcatgaa gatttcgaaa agacggatca ccctttccac cgcgggtatc
     1201 gttccgaaga tactggagtt gggtagtgct gggcagtccg cagggcagac ggtcttctcc
     1261 gcgcgcatga gagcgccacg tgtaaacctc gccatatctc tcaatgcaac taccgacaag
     1321 gtaagggaca ggcttatgcc cgtcaataaa acatacccga taagatccct cctggatgcc
     1381 tgcaggagat tccctttgga acccaggagg aggatcacct tcgaatatgt gatgctgagc
     1441 ggcatcaatg attccgctgc tgatgcgaag aggttaatca ccctcctaag gggaattccc
     1501 tgcaaggtga atctgattcc ttttaatcct taccggggat gtgagttcgg acgtccatca
     1561 gacgaaacgg ttctcacgtt tcaaaaaatt ctcctggatg cccatatgac cgcgctgatt
     1621 cggaagagca aggggcagga catcctcgcc gcgtgcgggc aactgaaagc ggggtacggt
     1681 taggaaccga tgaaggagtt caccagtttt atgagttcct gggtctggat cggctttgcg
     1741 aggtacgcgt tggcgccaat ggccagggcc ctctccttgt cctctttggc accctctgtg
     1801 gtgataatga ttacgggtat gtccttgtag gactgattgt tcttcacaag acttaccagc
     1861 ttgagcccgt ccattaccgg catattgata tccgcgagga tgagatctat tttttccgac
     1921 gagagtttct tgagcgcgtc aacgccgtcc gtggcctcta tgaccttggc gttggctatt
     1981 ctcttcatgg caaaggtgat gagctgtctc attgtgggcg aatcttcaac cacgagaatg
     2041 cttggcattt ttcctcctac ctctttggtt tctctttcag gagttcaagg aaactctgta
     2101 tagtggtcag tttccgttct gattgggtat agagctttgc agagaatatg gcggttgccg
     2161 cgtgccctgc caggaggttg aagagttcgt agtcaacgtt ggtaaacccc tccttttgcg
     2221 tgaggagaga gtatatggca atcactccga tcacatgctc tttgatcttc agtgcgatac
     2281 acacaatggg aagcccctgt gcaacatctc ccccgctcgt gatatcagcg gcgaaataac
     2341 tctccccctc tttcgcgacc gaccctatga tcccctcgcc gatccttgtc ttgggaaccg
     2401 actccagggg tatcccgtct gcggagaccg gcacaatatc attggtcttt tcgtctatga
     2461 ggagtattgc aaatgcctcc gcacctataa ggttgatcac aatttcgatg atgatcctca
     2521 gtacctcgtc aaagtccagc gtggaatgga gctgatagct tgccacatag agattcgcga
     2581 gtgcattgtt tatttcctcc acctccacgt atttggacgc aaagtccttg ttctcctctt
     2641 ctaccttcag atacctctcc ttgacggaat tgatctcctc gtccagtttc tttatacgtt
     2701 cctcatagag tcttattttc tcttccgcgg cggaactcag agtagcttct tccagttgtg
     2761 caaccctgaa gcgcagcttc tcgttctccc tgagaaggtc tttcgtgaac tcctcaccct
     2821 ttttgaatat ctcaaggaat tcgtccaccc ttcttgttat gactgggtct ttttccattt
     2881 tctgtccctc ttccctgcaa cggtcattat gatttcgccg ggtattttgt gaaggggcag
     2941 cacccggtca accgcccctg tctttatcgc ttcattgggc ataccaaaga tcaccgcagt
     3001 ggattctgcc tcggctatag tataccctcc ttttttctta atttccacta cccccgcctt
     3061 cccgtcgttt cccatccctg tgagtaccac ccccaccgtc atagggccga agtgctctgc
     3121 agcggatgac atcatgagat ccacggaagg cacatatttg tcgcgacccg ttgcctcctt
     3181 gagaatcaca cgaatcgtct gatgtttttt cttcaagatc atattatggc cccccgggca
     3241 tacgagggcc ttgcccggtt ctacaatatc accatcagca gcctctctca ccttaatgcg
     3301 ggtgagtttc gcaagcctct cagtaaacgg cttcgtgaag ccccttggca tatgctgact
     3361 cacaacaatt ccggccggga ggttccaggg aaggcgtgtg agcacgatct ggagcgcggt
     3421 gggtcctccg gtcgaggccc cgatcgctac cacgccgtgt tcctctgaag attcctcgga
     3481 tccctcatac acctcatctc tccgcgcttc attcggggag tgatggagca gcagctctct
     3541 ggaaatgctg agcttcccca tctcgaggtc ccttatcccc ttgatctttc ccaaaaggtc
     3601 accctgtat
//
LOCUS       DATKOU010000025        26289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_802751, whole genome shotgun sequence.
ACCESSION   DATKOU010000025 DATKOU010000000
VERSION     DATKOU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802751"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VMU21_00910"
     CDS             complement(<1..222)
                     /locus_tag="VMU21_00910"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HUO76111.1"
                     /translation="MKEIITVETIERKIYLVRGHKVMLDSDLAEMYAVATKVLLQAVK
                     RNLDRFPGDFMFQLNYQEVAVLRSQIVTSK"
     gene            complement(243..371)
                     /locus_tag="VMU21_00915"
     CDS             complement(243..371)
                     /locus_tag="VMU21_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76112.1"
                     /translation="MKYIEIIEEYWEKDIYCEAFENLIVRKGGEKKRGIDYSGANL"
     gene            complement(493..1596)
                     /locus_tag="VMU21_00920"
     CDS             complement(493..1596)
                     /locus_tag="VMU21_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HUO76113.1"
                     /translation="MQTRSVLWLSGVRRAGKTMLCQSLPETEYFDCELPRVRRMMEEP
                     QSFLEDLKGHTVILDEIHRLVNPSELLKIAADHFPETKIVATGSSALGASAKFKDTLA
                     GRKYELWLTPMITEDLKDFTQTDLSHRFIHGGLPPFFMAKRFPERDFQEWMDAFWAKD
                     ILELFRLERRHSFMKFAELLMIQSGGSFEASSFAGPCEVSRSTISNYLAVLEATYVVH
                     VIRPFTSHRSTEIVSAPKVYAFDTGFMCYYRGWDKLRQDDMGTLWEHFVLNEIMARTQ
                     SRRIHYWRDKQKHEVDFIVAKNKPEPTVIECKWTATDFDPANIKIFRRHYKRGLNFVV
                     VSDIKRSHSRNYGDVTVRFVSLEDLIKELAGQS"
     gene            complement(1811..2119)
                     /locus_tag="VMU21_00925"
     CDS             complement(1811..2119)
                     /locus_tag="VMU21_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HUO76114.1"
                     /translation="MSARKMTPVHPGEILLEEFLKPLGISQYRLSKDISVAPRRINEI
                     VHGKRAITADTALRLARYFGLSERFWLNLQSRYDLEVEKDKLDTRLEREVRVLELKSA
                     "
     gene            complement(2128..2409)
                     /locus_tag="VMU21_00930"
     CDS             complement(2128..2409)
                     /locus_tag="VMU21_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO76115.1"
                     /translation="MIKSFRGKDTEKLFLRQLSSKFPSEIRRSALRKLLVLDGAERLE
                     DLRVPPGNRLEKLTGNRHGQHSIRINDQWRICFRWHQGDAYDVEVTDYH"
     gene            complement(2501..3154)
                     /locus_tag="VMU21_00935"
     CDS             complement(2501..3154)
                     /locus_tag="VMU21_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HUO76116.1"
                     /translation="MNESFLSVSKTILHCERNLAEWHRGVSHYGFWAVLVDDQGWLEL
                     FEAARAHVERFLHPGYRRAPHITVAACGLLSEEHFSPTLQRHQATALNKANVPPFFLR
                     AAMLDSFASAPYITVEDQDGSLDTLRSILAMISQEDNPAEYKPHIALGLYQEAFETLL
                     VAHHLREFRSVATRQLPVTELAFCAYETSDLQGPFGVVERVALAGPDRTKGVLGSAP"
     gene            complement(3173..3249)
                     /locus_tag="VMU21_00940"
     tRNA            complement(3173..3249)
                     /locus_tag="VMU21_00940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3213..3215),aa:Met,seq:cat)
     gene            3389..3793
                     /gene="speD"
                     /locus_tag="VMU21_00945"
     CDS             3389..3793
                     /gene="speD"
                     /locus_tag="VMU21_00945"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HUO76117.1"
                     /translation="MHALGTHLLVELRDCNPTILKSLDEVREALVSAAKEAKATIVDI
                     SFHEFNPFGISGMVVIAESHLSIHTWPEYSYAAVDIFTCGDVIKPEVAAASLIERFES
                     KNPSVVEMKRGILSHENIKFPHKFSPPELQTV"
     gene            complement(3802..4995)
                     /locus_tag="VMU21_00950"
     CDS             complement(3802..4995)
                     /locus_tag="VMU21_00950"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HUO76118.1"
                     /translation="MNKITIEDLDIKGKRVFIRADFNVPLDENLKITDDRRIRSTLPT
                     INYAIDEGAKVIIGSHLGRPKGKVSPRASLAPVAKRLQRLLDKDVVFADDCIGPQVEH
                     LVSKMKNGDVMVLENLRFHPGEERNDEDFSKALAKLADCYVNDAFGAAHRAHASIVGI
                     TNLIPSAAGFLLKREIEYLKGVVTNPVRPFVAILGGAKVSGKIGVLENLVDKVDKVIV
                     GGGMAFTFIRAMGYEIGNSLVEADMLDFANTIREKLIRNNVKFYLPVDCVIAQSMEPG
                     IETKLVTTQEIPRGWLALDIGPASVKLFAEAIQDAKTVIWNGPMGVFELDAFSRGTYS
                     IAHSVADAYALTIVGGGDTDLAVHKAGVSDSISFISTGGGASLQLLEGKELPGIAALT
                     DRKKE"
     gene            complement(5053..6051)
                     /gene="gap"
                     /locus_tag="VMU21_00955"
     CDS             complement(5053..6051)
                     /gene="gap"
                     /locus_tag="VMU21_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HUO76119.1"
                     /translation="MPVKVGINGFGRIGRNFFRASKGVKGFEIVAINDLTDAPTLAHL
                     LKYDSIHGIFGAEVSAKENALVVDGREVKVTAECAPELIPWKSLGVDTVVESTGHFTD
                     REKAAKHIAAGAQRVIISAPAKEPDITVCLGVNEEMLDPAKHKIISNASCTTNCLAPV
                     AKVLHKEFGIIRGLMTTIHSYTNDQRILDLPHKDLRRARAAAVSMIPSTTGAAKAIGL
                     VLPELKGKLDGFAMRVTTPNVSVVDLTAELQKDTTADEINAAMKKWAAGPMKGVLQYV
                     DIPLVSVDFNGNSHSSIFDATLTKVMEKRMVKVIAWYDNEWGYSSRIRDLILFLTK"
     gene            complement(6062..7306)
                     /locus_tag="VMU21_00960"
     CDS             complement(6062..7306)
                     /locus_tag="VMU21_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HUO76120.1"
                     /translation="MAKVVPFRGIIYDTARVSGGDVVAPPYDIITPELREALYRKSPY
                     NIVRIDSGMENAGDNETENKYQRAAGYLDEWMKEGIFLQSPRPCFYVYEMDYRAEGTR
                     KRLRGFFGLVQLEELGKGVYPHEETHSKPKVDRLRLLSACKANTSPIFSLYHSDEKKA
                     SEILKKVVARPYMEAAGPDGAVHRLWVIDGESDLTAIRNDLADKAIYIADGHHRYETA
                     LEYQRIMREKTGGASGNEPFNYVLMFLTNIADGDITILPAHRIIRYPGKKITDLLSGH
                     FEIKTIPLEDDIIGVMRGKDQTLGLYQKGEKRYHLLKYKGDGMKETHPAVKGLDVTIL
                     HELVLKGLLGVSSVKYEMDQSLAQSMVREGDYDAVFYLNPTKVEDVERVALSSMRMPP
                     KSTYFYPKVMTGFIINSFRNSL"
     gene            7481..8593
                     /gene="tgt"
                     /locus_tag="VMU21_00965"
     CDS             7481..8593
                     /gene="tgt"
                     /locus_tag="VMU21_00965"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUO76121.1"
                     /translation="MLNFEIHSGDSGARTGLVQMSRGTIHTPAFMPVGTNATVKAMSP
                     EELRDMGAEIILGNTYHLSLRPGTEVIRAVGGLHRFMNWSRPILTDSGGFQVYSLSAL
                     RRIEPHGVHFRSHVDGSMHFIGPDEAMDIQSALGSDIAMVFDDCTPYPVEFGYALSSL
                     QLTLAWAKRCRELQKENQALFGIVQGSVYKDLRTMSINGLLDIGFDGYAVGGLSVGEP
                     KEIMHEMIHFVGPLLPKGKPRYLMGIGDLTDVLVAVDSGFDMFDCVMPTRNARNGTLF
                     TSLGRVSIKRTEYKTDEKPLDPECGCYTCRNYSRAYLRHMFLTREILSMRLNTMHNLF
                     FYMTFFRKMREAIARGEFQPFKKKSTAMAGAEESPG"
     gene            complement(8829..9497)
                     /locus_tag="VMU21_00970"
     CDS             complement(8829..9497)
                     /locus_tag="VMU21_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUO76122.1"
                     /translation="MKLVLFDIDGTLIDSGGAGTRSLNYAFEDLFSVHDAFKGISMAG
                     KTDIQILKEGLGRHDLDTENGNVGLLCERYIVHLRTEIGNPGKHLKPGIAESLGLLAG
                     RKDVSLGLLTGNIEPGARIKLDPFRLNAYFPLGAFGNDHEDRNKLLPIAVERFSELYQ
                     REIPYNDCVVIGDTPRDVECAKIYGAYSVAVATGPYSYASLLGSGADMVLRTMSEMNY
                     GVLG"
     gene            9539..10093
                     /gene="pyrE"
                     /locus_tag="VMU21_00975"
     CDS             9539..10093
                     /gene="pyrE"
                     /locus_tag="VMU21_00975"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HUO76123.1"
                     /translation="MDNKARLIEYIKRHSFQRSDTPIYKLSSGNMSSFYFNLKKTTYS
                     PEGQFLVGNLVFDKINELGWKPKAIGGLTMGADPIATATAYTSYIRNEPVEALVIRKE
                     PKGHGTMSQIEGKVMPGDKVIIIDDVVTTGGSTIKAIEAAKKAGLNIIGVIILLDRGE
                     SNGRQNIEDLGYPVHSILTIRDFT"
     gene            10123..10605
                     /locus_tag="VMU21_00980"
     CDS             10123..10605
                     /locus_tag="VMU21_00980"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HUO76124.1"
                     /translation="MDGYNLIGVYHKELETERNKLVDLLIEYRKRKGHYIVAVFDGWK
                     AGGATESSSVRGGVRIIYSRLGEKADSVIKRILSTERREWIVVSSDREIAAHAWSLGS
                     TPLPSEEFLPFLRSKARDDREEDWPEGEDDEEEGIPRTGNPNKLSKRERAVRRALGKL
                     "
     gene            10707..11492
                     /locus_tag="VMU21_00985"
     CDS             10707..11492
                     /locus_tag="VMU21_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUO76125.1"
                     /translation="MKTLIAIYATLFLIQTLLIRSAPFLNRNKATDIIRSVVFPVACV
                     LVIFDSLELLVHTINPRDIDPWLIRIDYVLLGGYPTVMLEALYTPLLTDILQLAYSSY
                     YLLPISFGLVLKLRGKEREFNKTVFLILLCFYLSYVGYLLFPALGPRYTMNHLQRFEL
                     RGLFVAEPIQRILNGLEGVKRDAFPSGHTAVVLVVSGLAYRFERGFFSLTLPVIILLI
                     FSTVYCRYHYVVDVVGGALLAAITLLVGDKYYGYWETRNRTHH"
     gene            11455..12159
                     /locus_tag="VMU21_00990"
     CDS             11455..12159
                     /locus_tag="VMU21_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO76126.1"
                     /translation="MDTGKPETVLIIEDEQKISDIVAMYLQREGFRTKVADTGEKALQ
                     LIHEPFDLIILDLMLPDIEGEELCKIVRERSDAPIIMLTAKSGEEDRIRGLGIGADDY
                     VVKPFSPRELIARVKAHLRRTRKMAKKLLSFNRGTLILDTENYEVSKKGVPTILTTTE
                     FRILMALAEKPGRVLSRLQLINMVQGYDFEGYDRTIDAHIKNLRHKIEDDPKDSQFIR
                     TVYGVGYKFSGVPDEE"
     gene            12149..13513
                     /locus_tag="VMU21_00995"
     CDS             12149..13513
                     /locus_tag="VMU21_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUO76127.1"
                     /translation="MRSKLFFAFLLVIVIALVSNLIFERLIMSDFDEYMKGTAEDHLS
                     WVLASIEGSYEGDRWDMNVVSEAIHWGMMLGFDMRVQDSAGREIVNSHMVMASLPPAM
                     MRRMESIVHTHSAEGEYEQYPLYNEGREMGTLFVRPLMKGGTIKVKEHIFKERGRNFL
                     VLSFVVAGLSAIAMAVFFSLYLSKPIRRLKLAAGKIAEGDFSIRVTPVSPDELGRLSE
                     SFNYMAEALQKEESLRKHLTSNIAHELRTPLTVMKAQMEGMIDKVVTDIPGGLENIRA
                     EVERLTKLVEGIEDLAKAEASFFSPGDYVRINLKEFLEGIVSGMAPIFHEKGLSFSLS
                     EKGDLEVITDVDKLDMIMKNVLSNSLKFTKEGGVRVEYGSKDSDFFVALRDTGQGIPG
                     DEISKIFTRFFRGTGAGDKGAGIGLALVKELIEVMGGRVDVKSSPGEGTTFTIWLPIK
                     GHAH"
     gene            13561..13854
                     /locus_tag="VMU21_01000"
     CDS             13561..13854
                     /locus_tag="VMU21_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HUO76128.1"
                     /translation="MQIPFKRTSDGIILMVRVLPRSSKKRIEITPEGLKVKLTAPPVD
                     GAANEQLIEILSEAFGIRRSAFRILKGLSSKDKVVGISGVDDVSNIHISFKLS"
     gene            13879..14193
                     /locus_tag="VMU21_01005"
     CDS             13879..14193
                     /locus_tag="VMU21_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76129.1"
                     /translation="MCRVFSAILFLMMTASLLYAAQEEGVAQYGDYTQWWCTVYGTRT
                     KEIGPAEAEQFIEKYFASKGLTAAHINHKGRFVEAEIYKNGRRLDVILFDRKTGRMRS
                     TY"
     gene            14242..14949
                     /locus_tag="VMU21_01010"
     CDS             14242..14949
                     /locus_tag="VMU21_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76130.1"
                     /translation="MKQELYELYQQGTELFDAGKYSEAEPILRDVIRANPNYADVLNK
                     LGIMAHLKGRYREASEYFERALEINPLYTEASLNLSITYNEMGEFKKAQEVFSLAAQI
                     AHPTPSSLDPFAAGKLANEHYKIGNIYLDFGMYDEAIAEYRKALGLHPKAADFHTKLG
                     VAIRSKGNFEEALVHFTKAKDLNPDYGQAWVQLGLTYYMKGMIGLAIEEWENALKYNP
                     KLKEAKTYLKLLRKEEL"
     gene            14955..15932
                     /locus_tag="VMU21_01015"
     CDS             14955..15932
                     /locus_tag="VMU21_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO76131.1"
                     /translation="MPLLEVRDLSVTFTTTSHPLTVVNTLNFAVQEAEVFGLVGESGC
                     GKSLTALAIMGLLPENARADGVLSFKGGNLLTLDRESIRRLRGKEISMIFQEPMTSLN
                     PVLTIGYQISEVITTHLGFSKKNAMEKTVDLLRAVKIPSPEIRVNEYPHQMSGGMRQR
                     VMIAMGIACNPSLLIADEPTTALDVTIEAQILELLRNLREERKMAVLLITHDIGVIAE
                     NADMAAIMYAGRIMELSRVSRVLENPKHPYTMGLLESLPKTKGVPLKPIRGSVPRPDA
                     LPRGCKFSDRCPYMIPDCVREEPRLREIVPGQHARCIRAEEITWESSRE"
     gene            15911..16885
                     /locus_tag="VMU21_01020"
     CDS             15911..16885
                     /locus_tag="VMU21_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HUO76132.1"
                     /translation="MGIIEGIDLRKYFPVGKGTFARHGWLKAVDGVSFALEKGTVFSL
                     VGESGCGKSTVARLLLRLTPPTSGRILFKDQNLFELKGDSLKAFRKSVQIIFQDPFAS
                     LNPRMTVFDTLAEPLKIHKLARKSEFKEKVANLLKGVGLQDEVMNRYPHEFSGGQRQR
                     ICIARALAVSPEIIVADEPLSALDVSIQAQILNILQELKQREDISFFFISHDLRVVQY
                     FSDEVAVMYLGKIVEYAKADELFAQPLHPYTAVLLASAPRIKPGKAKRTVLKGDVPSP
                     IHIPAGCPFHPRCPQRFEPCDKKVPLLQESASKGFRGRQVSCHLWNPY"
     gene            complement(16968..17471)
                     /locus_tag="VMU21_01025"
     CDS             complement(16968..17471)
                     /locus_tag="VMU21_01025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUO76133.1"
                     /translation="MRIILMLLTFLFLTAEAPSPWGIDELMGKKAPDFTLKDLNDKTV
                     SLSSLKGNVVLINFWATWCPPCRAEMSSFNNLYKEYKNNGLTIIAVSTDRSISSVKDF
                     LSKKPVEFPVLMDSDSKVSRRFKVFSLPTSFLLDKNGVIVQKFLGEEEWDSPEIKNKI
                     KDMLAVP"
     gene            complement(17484..18797)
                     /locus_tag="VMU21_01030"
     CDS             complement(17484..18797)
                     /locus_tag="VMU21_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76134.1"
                     /translation="MPIKIKLRERLRGEGLGLNPDLRFLLFVFLASFAIIALRRPDAI
                     FNPQFWAEDGKVFYADAYNKSLIASLLSPWGGYLDTFPRLIAAFSQIFPLSRAPLLFN
                     LAALVVKVLPVTLILSSRFYGLIPDFKTRLFLAFLYLALPNSWEINAGPLHGKTYLSL
                     LTFMVLSASPGSHLLSLFFDAGIILLSGLSGPFCILLAPISAIFWLHRRDKRYLVFFI
                     LIGICAIIQGTIFVTGSGRPQRLLGATPKLFVEILATQIFLGSVIGYKGLLLVSRGVL
                     ELISHFLTLYNLIVIVFGMLGLVALVNVMLKAPLELRLFVLYASLLFVAALLFPLGKP
                     NDPQWPLMLIPGVGGRHWFIPMLAFLSVLVWSLRKSSSRISRTLAVLAFVMMVPGIIL
                     DWQYPAFKDLNFREHVYRFESAPIGTQATIPINPPGWTMVLTKHL"
     gene            complement(18987..21608)
                     /locus_tag="VMU21_01035"
     CDS             complement(18987..21608)
                     /locus_tag="VMU21_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76135.1"
                     /translation="MFSVVMPVWNRSKVVGRAIESVLGQSYKDFELIVVDDGSDDVLD
                     NVVSPFLSDKVKYYRTEHQGVSAARNLGVRKATGELIAYLDSDNVWHSGFLARMVAVL
                     LSGEYDAAYCLARRFVRDQKGEIVEDGTLGREFCFKELLENNYIDLNTFVHTRRIFDL
                     KGGFDEKLRRLGDWDLIIRIAAWGEIKFVPEILVDYYYQIENNAISLIEDHESAKKAI
                     LARYPKIPEPITYEHDTIKYIWENISDRKYKNYWLRLQPGAINSLDYRAYGQPVVMQI
                     EPTNMCNLQCPLCPVAQKTLQRESRHMRFEEFKKIVDDVQDHALLLILWDWGEPLTNP
                     DLPLMIRYAADRDIRTVTSTNAHLLNDDKRVEEILSSGLSTLIVAIDSIHADIYQKYR
                     KKGSIEKALSGLRKTIEIKKRIGSSTTINLRMVVMRQNEHEVAGMERVARRIGADVFT
                     VKTLNPACGEVGLDSELVPKDPRYQRLEYRPGTWERVPIDRDCERPWIMANIYSDGSV
                     VPCCYDFDATMKVGNALEEPFSTIWNGPAFVAIRKKILTDRNNILHCTQCTINYKHTP
                     TGMFYRSVDFRDLKKRRLELLRAALIRLLSPVRTMAGTLIDLLSHLMAGVSRKDSAPL
                     STPPVQEPRLPIRVYPLRIPLPPGEKQGWTPYPIFNGSTAGVQSLASHVSVLTKGHCP
                     HPPHIHIEEEILLLLAGEVDLIFPDEQVPGGEQRKRLKPNQFVYYPTGFAHTLQTMSE
                     SPANYLMLKWHGVPTKIDTPLIFGQYDVFQPVQYSEARNGFCPRQVFEGPTAYLQKLH
                     CHVSTLSPGAGYDPHVDAYDVAIIILEGEVETLGERVGRCGVIFYRAGDAHGIRNPGE
                     AIARYLVFEFHGSQAER"
     gene            complement(21655..22989)
                     /locus_tag="VMU21_01040"
     CDS             complement(21655..22989)
                     /locus_tag="VMU21_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76136.1"
                     /translation="MDKRFDVQIPWIIEEDTELTLHIYDRRLLGSRRYFIRFDLYPIY
                     SPSHMNRHHGFWDIPLNYITREKTSLTFEKDELIVKDSMRPVRLKPVWKGDLEFIGYS
                     MLHISLWDFSKDPPVQLMVKSSQHVIRPGKSDLPLEQVCLPVTQRCNLSCHMCMRHNP
                     EDWDASDIKPEVLQPVLDASPFLYSVSAFGIGEPLLYENLFELIGELKKRMPENGQVG
                     FTTNGTLMNKRAASRLIDLGVNWLSFSIDGATKLTYESIRPGANFDEVIQNIAHTVQY
                     RNVSGRKRLWLVANYVIQNENVREIPAFARLAGSLGLDTVTFSHLRDFKAGEFRILGE
                     KLLAPLFEQAAEIGDRSGLKITFPRLHPLDESKCLFMQAAYLWVSGEVVPCCRMLKGA
                     YPGPIKAFGNVKRDSVLDIWNSAEYREFRHGVLTRDFPDECKGCNYATGLLG"
     gene            complement(23007..24206)
                     /locus_tag="VMU21_01045"
     CDS             complement(23007..24206)
                     /locus_tag="VMU21_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76137.1"
                     /translation="MRRHYGWWDIPLKYIKQESTSFVLDFNRRVISLENQKVPLEIKQ
                     GWRGLIEEDGYCMLHVSLWDNSKEPPHMIEVRSQPLVIAIKNLDLPLKQICLPVTDRC
                     NLKCTMCARQATDEIVDMDISDEALEPLLEAGSNFVSALLQGQGEPLLYPKIFSLIPR
                     IKDRMAAGGEVGLTTNATLLDEHTAARLLDTGIDFLYFSMDGASRKVYEAIRVGARFD
                     RVTENIRRCTRLQKTSGRNNLRIMLNFVILEQNLHEIPAFISLAAQLGVEHVTFSYCT
                     DTATGGLKVFGAEVLQELFHKAREEGKKLHMNVDTPPLRKNPKEICFFMERAVALLPG
                     EVFPCHAMAPGYKTLTRNRSFGDVRRTPLLEIWKRPDYQEFRRRTVRRDFPAECADCD
                     CKAYLVP"
     gene            complement(24354..25646)
                     /locus_tag="VMU21_01050"
     CDS             complement(24354..25646)
                     /locus_tag="VMU21_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO76138.1"
                     /translation="MSFDLLVRSYGEREPAELTLHVFEEYNPVALRKARHTLEFGSRV
                     KLQKFNEPFRFEPIRDSRGKTYSLVLGIGKDAERAGLLCSTLKGTLTVAGYRQHHHTQ
                     PVIIPYYSVSMRLRKYVKDNEAPLVSCLIVTYNSEGYVRHCLNSIAAQDYPNIEVVVI
                     DNHSDDHTVKIIREEFSHLNLFVMEQNLEFCKGNNFGLSKCKGEFICVLNADVVLEQG
                     AVRRFVEHIELSPHISIVGSTIETKGSRTRYADTFIIDGLITNNEKLLAGERFISAPC
                     GAGFMIRRSAVDDLDYLFDEGFISNWEDHDLGLRCWLHGHVVVHIPELGLYHYGGSAY
                     GLADPRRESQVFRNTLLTYFKNFGKRLFLRAFLRTLMSCTRPYRILGVMRFLGCFWKY
                     MPARAALQRKRKIDDSVLQVVTSGFLAVIPEDEKERGA"
     gene            26063..>26289
                     /locus_tag="VMU21_01055"
     CDS             26063..>26289
                     /locus_tag="VMU21_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76139.1"
                     /translation="MYTKGFLKAGDTSGTSENLSHEMLVGQQIVCIITEAVLSEHERR
                     MVDNVSYERISYQDKNTLVFIKEICISSAGD"
BASE COUNT         6639 a         6724 c         6529 g         6397 t
ORIGIN      
        1 ttttgaggtc acaatttgtg acctcaaaac tgcaacctct tgataattaa gctgaaacat
       61 aaaatcacca gggaaccggt ccagattgcg tttaactgcc tgaagcagta ctttcgtagc
      121 gacggcgtac atttcagcaa gatcactgtc gagcattacc ttatggccgc gtactaaata
      181 tatctttcgt tcgattgtct caacagtaat tatttctttc aattcctctc cttaaagcga
      241 agtcacaaat ttgcaccgga ataatcaata cctcttttct tctccccacc cttcctgaca
      301 atcaggttct caaatgcttc acagtatata tctttctccc agtattcttc aataatctca
      361 atatatttca agagtgggtt ttcaggcaga tagacattcc agactggaaa cagaaatcta
      421 agcgaacctt ctaattgccg gcgtctgccg tgtccggtat ccggcgatgg actggtacac
      481 tgaagggata gatcaagatt gccctgccag ctctttgatc aagtcctcaa gacttacaaa
      541 cctgacggtg acatcgccat agttccgaga atgcgatctc tttatatccg ataccaccac
      601 gaaattcagt cccctcttgt agtgtcttcg aaagatcttt atgttcgccg gatcaaaatc
      661 cgttgccgtc catttgcatt cgataaccgt cggttcaggc ttattcttcg cgacaatgaa
      721 atccacctca tgtttttgct tgtcacgcca atagtgaatc cttcgggact gggttcgcgc
      781 cattatctcg ttcaggacaa aatgctccca taaggtcccc atatcatcct gccggagctt
      841 gtcccatccc cgatagtagc acatgaaacc tgtgtcaaag gcatagacct tcggggcgga
      901 tactatctcc gtggaacgat gcgaggtgaa gggccggatc acgtgcacta cataggtcgc
      961 ttccagaaca gcaaggtaat tcgagatggt cgatctgctg acctcacagg gaccagcaaa
     1021 gctgctcgct tcaaaactcc ccccgctctg gatcatcagg agttcagcaa acttcatgaa
     1081 tgagtggcgt ctctccagcc ggaagagttc caggatatct tttgcccaga acgcatccat
     1141 ccattcctgg aaatcacgct cgggaaaccg cttcgccatg aagaacggag gaagccctcc
     1201 atggataaag cgatgggaca ggtcggtctg tgtgaagtct ttaaggtcct cggtgatcat
     1261 aggggtaagc catagctcgt atttcctgcc cgcaagggtg tctttgaact ttgccgaggc
     1321 ccccaaggcc gaagagcccg tggctacgat tttcgtttca ggaaaatggt cagcggcgat
     1381 cttcagcaac tcggaggggt tgaccaggcg atggatttca tccaggatta cggtatgccc
     1441 tttgaggtcc tcaagaaagg actgaggctc ttccatcatc cttctcacac gcggcagctc
     1501 acagtcaaaa tactcggttt ccgggaggct ttgacaaagc attgtcttgc ctgccctgcg
     1561 cacaccggaa agccagagga cggaacgcgt ctgcaatcct tctgctatct tcctgagcca
     1621 gaaactacgg tttaccatat gcaactataa tagcatatag taataccaaa tacaactgaa
     1681 cctgccgtga gactatcagg aatacatccc atttccccaa taaccttccc ctcgacactt
     1741 agagaccgtg tggtgaatcg cagaacgtga agtgttccct ttctcacttc atcatctatt
     1801 caatattgtg tcaggcgctt ttcaactcca gcaccctgac ttctctctcc aatctagtat
     1861 ccaacttgtc tttctcgacc tcaaggtcgt agcgcgattg aaggttgagc caaaaccgtt
     1921 ctgaaaggcc gaaatagcgg gcaagtctca gggccgtgtc agcagtgatt gcccgtttgc
     1981 catggacaat ctcattaata cggcgcggcg ctacgctaat atctttagat agcctgtact
     2041 gactgatccc caagggcttc agaaattcct caagcagtat ttcccccgga tgaacagggg
     2101 tcatttttct ggcgctcata attcctccta atggtagtcg gttacctcaa cgtcgtaggc
     2161 atccccctga tgccaccgaa aacatatcct ccactgatca ttaatgcgga tgctgtgctg
     2221 tccatggcga ttacccgtga gtttctctaa gcggttgccg ggcggtactc gcaggtcctc
     2281 aaggcgctcg gcaccatcca aaaccagcaa tttcctcaac gcacttcgtc gtatctcgga
     2341 ggggaatttt gatgacaact gcctgaggaa gagcttctcg gtatctttac cacgaaagct
     2401 ctttatcatg cgttaataat aacgcgtgga gataataatg tcaagcgtta tggaaaatat
     2461 tagggaatat cagggactca gcctattttc tcgatgaccc ctacggggca gaccccagga
     2521 ctcctttggt acgatcaggg ccggctaatg cgacacgttc cactacacca aatggaccct
     2581 gaaggtcact ggtctcatag gcacagaacg ccagctctgt tactggcagc tgcctggttg
     2641 ctacagaccg aaactcccga agatgatgtg ccaccaacag cgtctcaaag gcctcttgat
     2701 ataagcccag cgcgatatga ggtttatact cggccggatt atcttcctga gagatcatgg
     2761 ccaaaattga gcgaagcgta tcgagtgacc catcctggtc ttcgacagta atgtagggtg
     2821 cagatgcgaa actatccagc atggcagccc ttaggaaaaa cggcggcacg ttggctttat
     2881 tcagtgcagt cgcctgatgt ctttgaagtg tgggagagaa atgttcttca cttaacagac
     2941 cgcaggcagc caccgtgatg tgcggtgcgc gacggtaacc tggatggaga aatcgctcta
     3001 cgtgtgcacg tgcggcttca aaaagttcaa gccagccctg gtcatcgacc aggaccgccc
     3061 agaagccata atgtgaaaca cctctgtgcc attcggcaag attgcgctcg cagtggagaa
     3121 ttgttttgct gactgagaga aatgattcat tcatgtaagt gtaagaaaag aatggtggcg
     3181 gtgcagggaa tcgaaccccg gacactgcgg atatgagccg catgctctga ccaactgagc
     3241 tacaccgcct ctttttgtac gcgcaatgca ttatatcaaa aacgcgcggg aaaattctct
     3301 tgacaaacac caccaatttg ctatattgtt tttgtttaat aaaggagtgc ttcactcctg
     3361 agaagtttca ttggaggagg gtggaggatt gcatgcttta ggtacccatc ttttagtgga
     3421 attgagggat tgtaacccaa caatcctgaa gagcctcgat gaagtaagag aagctttggt
     3481 ttccgcagca aaggaagcaa aagccaccat cgtagatatt tccttccatg aatttaatcc
     3541 ctttggtatc agcggcatgg ttgtaatcgc ggagtcccat ctgtccattc acacctggcc
     3601 cgaatattcg tatgctgccg ttgatatctt cacgtgcgga gatgtgatca agccggaagt
     3661 ggctgctgca tcccttattg aacgctttga gagcaagaat ccgtctgtgg tggagatgaa
     3721 aaggggaatc ctctcgcacg agaacatcaa gttcccgcac aagttctccc ctccggaact
     3781 ccagacggta taggagcgct cctactcttt tttcctgtct gtgagcgccg cgatgccggg
     3841 gagctctttc ccttccagga gctgaaggga tgcgcctcca ccggtggaga taaaagatat
     3901 ggagtcagag acgccggcct tgtgaaccgc aaggtcggtg tcccccccac ccacaatggt
     3961 gagcgcgtat gcgtcagcta ccgaatgcgc aatggaatag gtcccgcgcg aaaaggcatc
     4021 aagctcaaac acccccatag gtccgttcca tatcacggtc tttgcgtcct ggatcgcctc
     4081 tgcgaagagc ttgaccgagg caggccctat atccagggca agccagcccc tggggatctc
     4141 ctgtgtggtc acgagcttcg tctcgatccc cggctccatg ctctgggcaa tcacacagtc
     4201 cacagggagg tagaatttta cattattcct gatgagcttc tccctgatgg tgttcgcaaa
     4261 atccagcatg tcagcctcca caagggaatt ccctatctcg taccccatgg cccgtatgaa
     4321 ggtaaaggcc attccgccgc ccacgatcac cttgtccacc ttatccacaa gattctccag
     4381 cacccctatt ttccccgaga cctttgcgcc acccaatatg gcaacaaagg gcctcaccgg
     4441 attggtcacg acccctttga ggtactcgat ctccctcttg agtaaaaagc cggctgcaga
     4501 gggaatgaga ttggtaatcc ccacaataga cgcatgggcc cggtgggcag ctccaaaggc
     4561 atcattcaca taacagtctg caagtttcgc aagtgccttc gaaaaatcct cgtcgttcct
     4621 ttcctctccc gggtggaacc tcaggttctc gagcaccatg acgtcgccgt tcttcatctt
     4681 tgataccaga tgctccacct ggggaccgat acagtcgtca gcaaagacca cgtccttgtc
     4741 cagaagcctc tggagcctct tcgcaacagg ggcgaggctt gccctcggac tcaccttgcc
     4801 ctttggcctg cccaggtgcg agccgatgat caccttcgcg ccttcgtcaa tggcataatt
     4861 gatggtcggg agcgtggagc ggatcctgcg gtcgtcggtg atcttgaggt tctcgtccag
     4921 gggcacgttg aaatcagcac gtatgaagac cctcttgccc tttatgtcca ggtcttcaat
     4981 ggtgatcttg ttcaatatgt cggttgcagg agaaggattt ccgttctttc tgatcatggt
     5041 ctttccctca ttttattttg taagaaaaag gatgaggtcc cggatccggg agctgtatcc
     5101 ccattcattg tcataccagg cgatgacctt caccatgcgc ttttccatca ccttggtgag
     5161 cgtggcgtcg aagatcgacg agtggctgtt tccgttgaaa tccaccgaga ccagagggat
     5221 gtccacatac tggagaaccc ccttcatagg gcccgctgcc cacttcttca tcgcagcatt
     5281 gatctcatct gcggttgtgt ccttctggag ttctgcagtg aggtccacga ccgagacatt
     5341 gggtgtagtg accctcatgg caaagccgtc gagcttgcct ttgagctcag gcagcacgag
     5401 cccgatcgcc tttgcagcgc cggtggtgga agggatcatc gagaccgcag ccgccctcgc
     5461 cctccgcaga tctttgtggg gaaggtcgag gatcctctgg tcgttcgtgt aggaatggat
     5521 cgtggtcatg aggccccgga tgataccgaa ctccttgtgg aggacctttg ccacaggcgc
     5581 aaggcagttg gtagtgcatg aggcattcga gataatttta tgcttggccg ggtccagcat
     5641 ctcttcgttc accccgagac agaccgtgat gtccggctcc tttgccgggg cagagatgat
     5701 cacgcgctgc gcccctgccg cgatatgctt ggcagccttt tccctgtcag tgaaatgtcc
     5761 tgttgattcg accacagtat caacaccaag ggatttccat ggaataagtt ccggagcgca
     5821 ctctgcggtg acctttacct ctctgccgtc aaccacaagg gcattctcct ttgccgagac
     5881 ctcggcacca aatatgccgt gtatggagtc gtacttgagc agatgcgcga gggttggtgc
     5941 gtcggtaagg tcattgatcg caacgatctc aaaacccttg acccccttgc tcgccctgaa
     6001 aaaattcctt ccgatcctcc cgaaaccatt aatcccgacc tttactggca tttcgtcctc
     6061 cttataggga attcctgaaa ctgtttatga taaatccggt catgaccttt ggataaaagt
     6121 aggtggactt gggcggcatc ctcatcgaag aaagggctac cctctccaca tcctcaacct
     6181 tcgtggggtt caggtaaaag accgcgtcat agtctccctc acgcaccatg ctctgcgcca
     6241 gggactggtc catctcatac ttgaccgagg aaacccccaa aaggcctttc aggaccagct
     6301 cgtgcagtat ggtcacatca agacccttca ctgcaggatg cgtctccttc attccgtcac
     6361 ctttgtattt caacaggtgg tagcgcttct cgcccttttg atacagacca agggtctggt
     6421 ccttccccct cattacccca attatatcat cctcaagggg gattgtcttt atctcgaagt
     6481 gccctgacag gagatccgta atcttcttcc ccggataccg tatgatacgg tgcgcgggaa
     6541 ggatcgtgat atcaccatca gcgatattgg tcagaaacat caacacatag ttgaacggtt
     6601 cgttcccgct tgctccccct gtcttttccc tcatgatcct ctggtactcc agtgcggtct
     6661 cgtacctgtg gtgtccgtcc gcaatataga tggccttgtc cgcaaggtca ttgcgtattg
     6721 ccgtaaggtc gctctctccg tcaatcaccc agagcctgtg aaccgcgcca tcaggacctg
     6781 ctgcttccat ataaggcctg gcaaccacct tttttaaaat ctccgatgcc ttcttttcat
     6841 cgctgtggta aagggaaaag atggggctgg tgtttgcctt gcaggcgctc aggagcctca
     6901 gcctgtccac ctttggtttg gagtgcgtct cctcatgggg atagacccct ttccccagct
     6961 cttccagctg cacaaggcca aaaaaacccc gaagcctctt cctcgtcccc tcagcccggt
     7021 aatccatttc atacacgtaa aagcagggac ggggagactg caggaatatg ccctccttca
     7081 tccattcatc aagatatccg gctgccctct ggtacttgtt ctctgtttca ttatcaccgg
     7141 cattctccat gccggagtcg atcctcacaa tgttgtacgg actttttctg tagagggcct
     7201 cacgaagctc cggggtgatg atatcatagg gcggagcgac cacatctcct ccggaaaccc
     7261 tggcagtatc atagataatt cccctgaaag ggacgacctt ggccatttca ctcctttgcg
     7321 aaagggaact cctgcgtcta cacacgattc ccctcaaaga gatttgtggg agaagcccca
     7381 caccagtcaa gaaaaaacac gaatatctta gcatgcaggg aacgggaatt tcaaaccaga
     7441 taagggataa gtgtgggagg tgtgtggtat tattttggtt atgctgaact tcgagatcca
     7501 tagcggggac agcggggccc gcaccgggct tgtccagatg tcccggggca ctatccatac
     7561 ccctgcattc atgcctgtgg gaacaaacgc gacggtcaag gcgatgagcc cagaggagct
     7621 cagggatatg ggcgcagaaa taatcctcgg caacacctat catctctccc tcaggccagg
     7681 gaccgaggtg atcagggcag tgggggggct tcacaggttc atgaactgga gcaggcccat
     7741 actcaccgac agcggcggat tccaggtcta cagcctctct gcgttacggc gcatcgaacc
     7801 ccacggagtg catttcaggt cccatgttga cggttccatg catttcatcg gccctgatga
     7861 ggctatggat atccaatctg cccttggttc tgatatcgcc atggtatttg atgactgcac
     7921 tccctatccc gtggaattcg gatacgcgct cagctctttg cagctgaccc tggcatgggc
     7981 gaagcgatgc agagagctcc agaaggagaa tcaggccctg ttcggcattg tccagggttc
     8041 tgtatataag gatttgcgca ccatgagcat caatggactt ctggacatag gatttgacgg
     8101 atacgcagta ggaggcctga gcgtgggtga accgaaggag atcatgcacg agatgataca
     8161 ctttgtgggg ccccttctgc ccaagggcaa acccagatat ctcatgggca tcggagatct
     8221 cacggatgtc ctggtcgcgg ttgattcggg atttgacatg tttgactgcg tgatgcccac
     8281 ccggaatgca aggaacggga cactattcac cagcctcggg agggtgagca tcaaacgcac
     8341 ggaatacaag accgatgaaa aacccttaga tcctgagtgc ggatgttaca cctgcaggaa
     8401 ttattcaagg gcatatctcc ggcatatgtt cctcaccaga gagatcctct ccatgcggtt
     8461 gaataccatg cacaacctct ttttttatat gaccttcttc aggaagatga gggaggccat
     8521 tgcaagaggt gaattccagc cattcaagaa aaagagcacg gctatggcag gcgccgaaga
     8581 gtctcccgga taagggtgtc ttttctcacc ggttcagcgg aacattagcc ttgataattc
     8641 ataaatccgg ctgaagtgtt cgctcgaatg gcaagactcg gggttatgtg tatgtccgca
     8701 agggcggact ttggcaatac atggggatag agcggtgtta ggtgtgcggg cccaggtagg
     8761 gtcgggaagg gctattgtct tgcaagagac gggcgcctct gccgggacaa agaactcatg
     8821 aataaagact aaccgagtac cccgtaattc atctcagaca tggtcctgag caccatgtct
     8881 gcgcctgacc caaggaggga tgcgtaggaa taaggccctg ttgccacggc aacggaatat
     8941 gcgccataaa tcttcgcgca ctccacatcc ctgggtgtat cgccaataac cacacaatca
     9001 ttgtaaggga tctccctctg gtagagctcg ctgaaacgct caacggcaat aggcaaaagt
     9061 ttgttcctgt cttcatggtc attgccgaat gccccaaggg ggaaataggc attaagtctg
     9121 aaggggtcca gttttatcct cgcccctggc tctatgtttc cggtgagcag tccgagggaa
     9181 acgtccttcc tcccggcaag gaggccgagg gattccgcga taccgggttt gaggtgtttc
     9241 cccggattcc cgatctctgt ccggagatgg acaatataac gttcgcagag aagaccgaca
     9301 ttgccgtttt ccgtatccag gtcgtgcctt ccgaggccct ccttcagaat ctggatgtcg
     9361 gtcttgcccg ccatgctgat gcctttgaat gcgtcatgga cagagaacag gtcctcaaag
     9421 gcatagttca gggacctcgt gcctgcccct cctgagtcaa tcagcgtgcc gtcaatgtca
     9481 aagagtacta acttcatttt ttgaattttc ccttgcgtat ttgctatatt ttatcataat
     9541 ggacaataag gcgcggctca tcgaatacat aaaaaggcat tcgttccaga gaagcgacac
     9601 acccatctat aagctctctt caggaaatat gagttctttc tatttcaatc tcaagaagac
     9661 cacctactca cctgagggcc agttcctggt gggcaacctg gtctttgaca agatcaatga
     9721 actggggtgg aaacccaagg caatcggcgg tctcaccatg ggtgctgacc ccatcgctac
     9781 tgcaacagcc tatacctcgt atatccggaa cgaacctgtg gaggcgctgg tcatcagaaa
     9841 agagccgaag ggccacggaa ccatgagcca gatcgaggga aaggtgatgc cgggtgataa
     9901 ggtaattatc atcgatgatg tggtcaccac cgggggatcg accataaagg ccatagaagc
     9961 cgcaaaaaaa gcgggattga atatcatcgg ggtcatcatt ctcctggaca ggggtgaatc
    10021 caacggcaga cagaatatcg aggacctcgg ttatcctgtg cattccatcc tcaccatcag
    10081 ggacttcacg tagagggagg ggcgcgtatt ccttccctga tcatagacgg atataacctc
    10141 atcggggtgt atcataagga actcgagacc gagaggaaca aactcgtaga tctgctcata
    10201 gaataccgca agaggaaagg acactacatt gtggcggttt ttgacggatg gaaggcagga
    10261 ggagccacag aaagctcatc ggtgcgcggc ggcgtcagaa tcatctattc acgattgggg
    10321 gaaaaggctg attcagtcat aaaaaggata ctctccacag agaggcggga atggattgtg
    10381 gtgagctcgg accgtgagat cgccgcacac gcatggtcac tcgggtccac cccgctgccc
    10441 tcagaggagt ttctcccatt tctcaggtcc aaggcacggg atgaccggga agaggattgg
    10501 ccggagggag aggatgacga agaggagggg ataccaagga ccggtaatcc taataagctg
    10561 tccaagaggg aacgggcagt gagaagggct ctgggcaagc tctgatgagg atcatttccc
    10621 agggaaaacc ggcagactct cttacccttt tcttccttat atgcctcacg gtagtatccc
    10681 tctattttca taggaggatc ccccatgtga aaaccctcat cgccatatat gcaacccttt
    10741 ttctcattca gacactcctg atacgatcag cgccattcct gaacaggaat aaggctacgg
    10801 atatcatacg cagcgtggtg ttcccggtgg catgcgttct tgtgatcttc gacagccttg
    10861 agctccttgt gcatactatc aatcccaggg acatagaccc atggcttatc aggatcgact
    10921 atgtgctcct cggcggctat cccaccgtga tgctggaggc tctctacacc cctctcctta
    10981 ccgacatact ccagcttgcg tactcttcgt actatctgct tcccatctcc ttcggtctcg
    11041 tattaaaact caggggaaaa gaaagggaat tcaacaagac cgtgttcctc atcctcctct
    11101 gcttttacct ttcctatgtg ggctatctgc tgttccctgc cctcggtccc cgctacacca
    11161 tgaatcatct ccagcgcttt gaattgaggg gcctgttcgt tgcggagccc attcagcgca
    11221 tcctcaacgg acttgagggg gttaagagag acgcctttcc cagcggacat actgcagtgg
    11281 tgctcgtggt gtcaggcctc gcctacaggt ttgagagggg cttcttctct ctgacacttc
    11341 ccgtgattat cctcctcatc ttttccactg tgtattgcag gtatcattat gtagtggatg
    11401 ttgttggtgg cgcattgctt gcagccataa ccttattagt cggagacaaa tactatggat
    11461 actgggaaac cagaaaccgt actcatcatt gaggacgaac aaaagatatc tgatatcgtg
    11521 gccatgtatc tccagaggga ggggttcaga acaaaagtcg ccgacacggg agaaaaggcc
    11581 ctgcagctca tccatgaacc ctttgatctc atcatcctcg acctcatgct ccctgatatt
    11641 gaaggagagg agctctgcaa gatcgtacgg gagcgatccg acgcgcccat tatcatgctc
    11701 accgcaaaaa gcggggaaga agacaggata cgggggctcg gcataggggc cgatgattat
    11761 gtggtaaaac ccttcagccc gagggagctc atcgcaaggg tgaaggcgca tctgagaagg
    11821 acgagaaaaa tggcaaagaa gttgttgagt ttcaacaggg gcactctcat cctggataca
    11881 gagaattacg aggtgagcaa gaagggtgtg cccacgattc tcaccacaac cgagttcaga
    11941 atattgatgg cccttgccga aaaaccggga agggtgctct cccggctcca gctcatcaat
    12001 atggtgcagg gatacgactt tgaggggtat gaccgtacca tcgatgccca cataaaaaac
    12061 ctgaggcata agatagagga tgacccaaaa gattcacagt ttatcaggac ggtctacgga
    12121 gtgggataca aattctcggg ggtccctgat gaggagtaaa ctttttttcg catttctcct
    12181 ggtgattgtc atcgcccttg tctcgaacct catctttgag cggctcatca tgagtgactt
    12241 cgatgaatac atgaaaggca cggcggaaga ccatttatcc tgggttctcg catccataga
    12301 ggggagttac gaaggagacc ggtgggatat gaacgtggta tccgaagcaa tacactgggg
    12361 aatgatgctc ggatttgaca tgcgggttca agactccgca ggcagagaga tcgtgaactc
    12421 ccatatggtc atggcatcgc tgccccctgc catgatgcgg aggatggaat ccattgtgca
    12481 cacccactct gccgagggag aatacgagca ataccccctg tacaatgaag gaagggaaat
    12541 gggaacgctc ttcgtaaggc cattgatgaa gggaggaacc ataaaggtga aggagcacat
    12601 cttcaaagaa aggggcagaa atttcctcgt gctctccttt gtggtcgcgg gcctgagtgc
    12661 catcgccatg gcggtctttt tcagcctgta cctttcgaaa cccatacgga gactgaagtt
    12721 ggcggcagga aaaatcgcag agggggattt cagcatcagg gtgacaccag tatccccgga
    12781 tgagttagga aggctttccg agagctttaa ttacatggct gaggcactcc agaaagagga
    12841 atctctaaga aagcacctca cctccaatat cgcgcatgaa ctgagaacac cacttaccgt
    12901 gatgaaggcg cagatggaag ggatgataga caaagtagta acggacatcc ccgggggact
    12961 cgaaaacatc agggcagagg tggaaaggct gacgaagctt gtggaaggga tcgaagacct
    13021 tgcaaaggcc gaagcgagtt tcttttcccc gggtgactac gtccggatca acctgaagga
    13081 gttcctggag ggtatcgtat ccgggatggc gcccatattc catgagaagg gtctcagctt
    13141 ttccttgtcg gaaaaagggg atctggaggt gatcactgat gttgacaagc tcgacatgat
    13201 catgaaaaat gtcctttcca attccttgaa attcaccaag gaaggaggcg tcagagttga
    13261 gtacggcagt aaagacagtg atttttttgt ggcgttgaga gacacggggc agggaatacc
    13321 cggggatgag atctccaaaa tattcacgag gttcttcagg gggacaggtg caggagacaa
    13381 aggtgcaggc atagggctcg cccttgtaaa ggaactcatt gaagtgatgg ggggaagggt
    13441 tgacgtaaag agctctccag gtgagggaac aacattcacc atatggctgc cgattaaagg
    13501 tcacgcacat tgaaggtgag taagcttttc ccgtggtggg ccggcatacg caggtgagat
    13561 atgcagatac cttttaaaag gacgtcggat ggtattattc tcatggtgag ggtgttaccc
    13621 cgctcatcga aaaaaaggat cgagatcacc cctgagggtc tgaaggtgaa gctcaccgct
    13681 ccccctgtgg acggcgctgc gaatgaacaa ttgatagaga ttctgtcgga agcctttggc
    13741 atacgaagaa gcgcatttcg gatactcaaa ggtctttcat ccaaagacaa ggtggttgga
    13801 atcagcgggg tggacgatgt gtccaatatt catatttcct tcaaactttc ttagttaaat
    13861 atgacaagga gcgtttacat gtgtcgggta ttttcagcga ttctgttctt gatgatgacg
    13921 gcatctctcc tttacgctgc tcaggaggag ggtgtcgctc agtatggtga ttatacgcaa
    13981 tggtggtgta cggtgtacgg tacgcgtaca aaagaaattg ggccggctga ggcagagcag
    14041 ttcatagaaa agtattttgc gagcaagggg ttaaccgctg cacatatcaa ccacaagggg
    14101 aggttcgtag aagcagagat atataaaaat ggcaggcgtc tcgatgtaat cctctttgac
    14161 aggaaaacgg gaaggatgcg gtcaacctat tagtccattc tcttgacaaa gaaactgttg
    14221 acgccctata ctcacaaagg catgaagcaa gaactgtacg agttgtatca gcaagggaca
    14281 gagctttttg acgcaggaaa atattccgaa gcagaaccca tcctgagaga tgtaatacga
    14341 gccaatccaa actatgcgga cgtactaaat aagctcggca tcatggcaca cctcaagggg
    14401 agataccggg aggcatccga gtactttgag agggcccttg agataaaccc tctgtataca
    14461 gaggcctctc tcaacctgtc catcacgtac aatgaaatgg gtgaattcaa gaaggcccag
    14521 gaggtttttt ctctggcagc gcaaatcgcc catcccactc cctcatccct tgatcccttt
    14581 gctgcgggaa agctcgcaaa tgaacattac aagatcggca atatctatct tgactttggt
    14641 atgtatgatg aggcgatagc ggaataccgt aaggcgctgg ggcttcatcc gaaggccgct
    14701 gatttccata ctaagcttgg tgtcgccatc aggagcaaag gcaattttga agaggccctt
    14761 gtccatttta caaaggcaaa ggatctcaat cctgactacg gccaggcttg ggtgcagctt
    14821 ggtcttacct actatatgaa aggcatgata gggcttgcca tagaggaatg ggagaatgcg
    14881 ctgaagtaca acccaaaact gaaagaggca aagacctacc tcaagcttct gagaaaagag
    14941 gaactgtaag cgcagtgccc ctccttgagg tcagggatct ctccgttacc tttaccacca
    15001 catcccatcc tttaacagtc gtcaataccc tgaactttgc agtgcaagag gcagaggtct
    15061 ttggccttgt gggtgaaagc ggctgcggga agagcctcac cgccctcgcc attatgggac
    15121 tacttcccga gaatgcacgc gcagacggcg tgctctcctt caaaggtggg aacctgctca
    15181 ccttggatag agaatccatc agaaggctcc ggggcaagga gatctccatg attttccagg
    15241 agcccatgac ctccctcaat cctgtactca ccatcgggta ccagatatcg gaggttatca
    15301 ccacccacct tgggttttcg aagaaaaatg caatggaaaa gaccgtagat ctcttaagag
    15361 cggtgaagat tccttcccct gagattaggg tgaatgagta cccccaccaa atgtccggcg
    15421 gtatgcgcca acgggtgatg attgccatgg ggattgcgtg caatccctcc cttctcattg
    15481 ccgatgaacc cacaacagcc ctggatgtaa ccatagaggc acagatactg gaactcctga
    15541 ggaacctgag agaggaacga aaaatggcgg tattgctcat tacgcacgac ataggggtaa
    15601 ttgcggagaa cgcggacatg gccgcaatca tgtacgcggg aaggatcatg gagttgtccc
    15661 gggtctccag ggtcctggag aaccccaaac acccctacac catgggactt ttggagtccc
    15721 tgcccaagac caagggagtg cccctgaaac ccatcagggg atctgtgcca aggcccgatg
    15781 cacttccccg gggctgtaag ttttctgaca ggtgccccta tatgattcct gactgcgtaa
    15841 gagaagagcc ccgcctgaga gagatcgtcc cgggtcagca cgcgcgctgt ataagggcag
    15901 aagagattac atgggaatca tcgagggaat agatctcaga aaatactttc ccgtggggaa
    15961 gggcacgttt gcacggcatg ggtggctcaa ggccgtggac ggggtttcct ttgcgttaga
    16021 aaaaggcacg gtattttccc tcgtaggtga aagcggctgc ggcaaatcca ccgtggcaag
    16081 gctccttctc cgattaaccc cccccacttc agggagaatt ctcttcaaag accagaacct
    16141 gtttgagctg aagggggact ccctaaaagc attcagaaaa tcggtccaga taatcttcca
    16201 ggaccccttt gcatccctca atccgagaat gacggtattc gataccctcg cagaaccact
    16261 caagatacat aagcttgcga gaaaatccga attcaaggag aaggtggcga acctgttgaa
    16321 gggtgtgggg ctccaagatg aagtgatgaa ccgatacccc cacgaattca gcggaggcca
    16381 aaggcagagg atttgtatcg cgagggccct cgcggtctct cccgagataa tcgttgcgga
    16441 tgagcccctt tccgcactgg atgtctcgat acaggcccag atattgaaca tcctccagga
    16501 actcaaacag agggaggata tttcattctt ctttatcagt catgatcttc gggtagtcca
    16561 atatttcagc gatgaggtgg cagtgatgta cctcggaaag atcgtggagt acgcgaaggc
    16621 tgatgaactc ttcgctcagc cccttcatcc ctacaccgca gtactcctcg cgtcggcccc
    16681 aaggataaaa ccaggaaagg ccaaaaggac cgtgcttaag ggggatgtgc cgagccccat
    16741 acacatacct gcgggatgtc cttttcaccc aaggtgcccc caacgattcg agccttgtga
    16801 taaaaaggtg cccctgcttc aagagtctgc gtcaaaggga ttcagaggca gacaggtctc
    16861 ctgccatctt tggaacccct actgaacgag atgcccaatc ttccggggac atcccttcac
    16921 cgggggtttt tggcaggaaa attgagatgc agtgccaggg tagtcctcta gggaactgca
    16981 agcatatctt tgatcttgtt ctttatctca ggtgaatccc attcttcttc acccagaaac
    17041 ttctgcacga taacaccatt cttgtcgagc aggaacgacg tggggagaga aaataccttg
    17101 aaccgtcttg ataccttgct gtcagaatcc atgagcacag gaaattccac gggtttctta
    17161 ctcaagaaat ccttgactga tgatatgctc ctgtctgtgg ataccgcaat aatggtaagc
    17221 ccgttgttct tatactcctt atagagatta ttgaaagatg acatctccgc acggcagggg
    17281 ggacaccatg ttgcccagaa gtttattaat actacattcc ctttcagtga tgaaagggag
    17341 accgtcttgt cattgagatc cttgagcgta aagtctgggg cctttttccc cataagttca
    17401 tcaatccccc atggtgaggg ggcctctgcg gtgagaaaga gaaaggtcaa cagcatcagt
    17461 ataattcgca tgcatacatc ttatcataaa tgcttggtaa gaaccatcgt ccagccgggc
    17521 gggttgatgg gaattgttgc ttgggtaccg ataggtgccg actcaaaacg atacacatgt
    17581 tccctgaaat ttaaatcctt gaaggccgga tattgccaat caagaataat gccgggcacc
    17641 atcattacaa aagccagcac agcaagagtt cttgatatac gcgagcttga tttacggagt
    17701 gaccagacca gaacacttag gaaggcgagc atcgggatga accagtgtcg tccgccgaca
    17761 ccgggtatta acattaatgg ccactgagga tcgttcggtt ttcccaatgg aaatagcagg
    17821 gcggcgacaa agagcagtga ggcatagagg acaaaaagcc gcaattccag aggagctttt
    17881 aacataacat tgaccaatgc tacaaggccc aacatgccaa acactatcac aatcaggttg
    17941 tacagtgtta agaagtgcga tatcaattcg agaactcctc gactaaccaa aagaagacct
    18001 ttgtaaccga tcacagagcc cagaaagatc tgtgtcgcga gtatttcaac aaataatttt
    18061 ggcgtggctc ctagcagccg ttgtggtcgc ccggaaccag tcacaaaaat tgtgccttgt
    18121 atgattgcgc agattccaat caatataaaa aagacaagat atctcttgtc tcgacgatga
    18181 agccagaaga tcgcggaaat gggggctaat aagatacaga aaggaccgct gaggccagac
    18241 agaaggataa tccctgcgtc aaaaaaaagg gatagcagat gactgccggg tgaagccgac
    18301 agaaccatga aggtgagaag agaaagatag gtctttccat gtaaagggcc agcattaatc
    18361 tcccacgaat taggaagggc aagataaaga aatgcgagaa ataacctggt cttgaagtct
    18421 ggtattaacc cgtaaaaacg tgaggataga atcaaagtga caggcagtac cttaactacc
    18481 agtgcagcga gattgaacag caagggcgca cgcgagaggg ggaagatctg ggagaaagcc
    18541 gcaatcagtc ttgggaaggt atctaaatac ccgccccatg gcgaaaggag cgaagcgatc
    18601 aaactcttat tgtatgcatc tgcatagaat accttgccgt cctcggccca aaactgcgga
    18661 ttgaagatcg catccggccg tctgagtgca atgattgcaa aggaggcaag aaacacgaag
    18721 agcaagaagc gcaggtcagg attcaacccc aacccttctc ctctcaaacg ctcgcgcaat
    18781 tttattttta tcggcattaa tgtttcagcc aagcaagccg gtcgcgtaat tacatccctt
    18841 gcattcgtcg ggaaaatctc gggtaagaac cccgtgtctg aattctctgt actctgcgct
    18901 gtttcagata tccaaaaggg accctcttca catcgtcgga tatcttaatt gtcccggata
    18961 agccctcttg cgagaagggg cacatcctat cgctccgcct gactaccgtg aaattcaaag
    19021 acaagatacc tggctatcgc ctcaccggga ttgcgtatac cgtgagcgtc acccgcccgg
    19081 taaaagatca ctccgcatcg ccccacgcgt tcaccgagtg tttcgacctc accttccagg
    19141 ataataatag cgacatcgta agcatcgacg tgaggatcgt agccggcccc cggtgaaaga
    19201 gtagagacat ggcaatgaag cttttgcagg taagccgttg gtccttcgaa cacctgacgc
    19261 ggacaaaagc cattcctggc ttcagagtac tgcacaggtt gaaacacgtc atattgaccg
    19321 aaaatcagcg gcgtatcgat tttagttgga acaccgtgcc actttaacat caggtagttc
    19381 gccggagact cgctcatggt ctggagcgtg tgggcaaaac cggtcgggta gtagacgaat
    19441 tgattgggtt tcaaacgttt tcgctgctct cccccaggaa cttgctcatc cgggaatatc
    19501 agatcgacct cgccggccag aagaagcagt atttcttcct ctatatgtat gtgcggcgga
    19561 tgcgggcaat ggcccttggt taacactgag acgtgactcg ccaaactctg cacgcctgcg
    19621 gtggacccgt taaagatggg ataaggtgtc cagccttgct tttcaccagg cgggagcggg
    19681 attcggagcg ggtaaacccg gattggcaac cggggttcct gcacgggagg ggtcgacagg
    19741 ggtgctgaat ctttgcgact tactcctgcc attaagtggc tcaagagatc tatcagagtc
    19801 ccggccattg ttctcacggg actcagcagt cggataagag ctgcacgtaa cagttccaat
    19861 cgtcgctttt tcaagtcccg aaagtctacg gagcgataaa acatgcctgt tggcgtatgc
    19921 ttgtaattga tcgtacattg tgtgcagtga agaatgttat ttctgtcggt aaggattttt
    19981 ttccgtattg cgacaaaggc cggtccattc catatcgttg agaaaggttc ctcaagggcg
    20041 ttacccacct tcatggttgc gtcaaagtca tagcaacaag gcaccacgct tccgtccgag
    20101 taaatgttcg ccataatcca cggccgctcg caatcccggt caatcggcac cctttcccaa
    20161 gtacccggcc ggtattcaag tctctggtac cgggggtctt tcggtactaa ctcactatcc
    20221 agtcccactt ccccacaggc aggattgagt gtttttacgg taaaaacatc tgccccgatt
    20281 cttcttgcca ctctttccat cccggccact tcgtgctcgt tttgccgcat caccaccata
    20341 cgaagattta tggttgtcga ggaacctatt ctctttttga tttcaatcgt cttacgcagt
    20401 ccggagagag ctttttcgat ggaacctttc tttctgtact tctgataaat gtctgcatgt
    20461 atagagtcaa tggcgactat gagggtggac agtccggaag agagtatttc ctcgacccgc
    20521 ttatcatcat tcagaagatg cgcgtttgtg cttgtcactg tgcgtatgtc cctgtcggct
    20581 gcgtaacgta tcatcagggg aagatcgggg ttcgtcaagg gctcgcccca atcccacagg
    20641 atcaagagaa gggcgtggtc ctgcacatca tcaacaatct tcttgaattc ctcaaaccgc
    20701 atgtgccggg attctctttg gagcgttttt tgggccacag ggcatagcgg gcattggagg
    20761 ttgcacatgt tcgtcggttc aatttgcata actaccggct ggccataggc cctgtagtca
    20821 agagaattga tcgcccccgg ttgaagcctc agccaataat ttttgtattt cctatcagaa
    20881 atgttttccc atatatattt tatggtgtca tgttcatagg ttatcggttc tggaatcttc
    20941 ggatatctgg cgaggattgc cttcttcgca gattcatgat cttcgataag ggatatggca
    21001 ttgttctcaa tctggtaata gtagtccacc agtatctcgg gaacgaattt gatctcaccc
    21061 caggctgcaa tccgtatgat caggtcccaa tcaccgagtc tgcgtagctt ctcgtcaaag
    21121 cctcctttaa ggtcaaatat ccttcttgtg tggacaaagg tgttaaggtc aatgtagtta
    21181 ttttccagga gttccttgaa acagaactcc cggcccaggg tcccgtcttc tactatttcc
    21241 cctttttgat ctcttacgaa ccgtcttgcc aaacaatagg ctgcgtcgta ttccccactt
    21301 aagagaacgg ctaccatccg tgccagaaag ccgctgtgcc acacattgtc tgaatcgaga
    21361 taggctatta actctcctgt ggccttccgt acccccagat ttctggcagc cgacactccc
    21421 tggtgttcgg tccggtaata tttcacctta tccgataaga acggacttac cacattatca
    21481 aggacatcgt ctgagccgtc gtcaacgacg ataagctcaa aatccttata actttgtccg
    21541 agaacgctct caatcgccct tcccacgacc tttgaacggt tccacacggg cattacaaca
    21601 ctaaacattt gttgttccct tgtttgagtc tgttttttgt ttataatcac tgtttcagcc
    21661 aagcaagccg gtcgcgtaat tacatccttt gcattcgtcg ggaaaatctc gggtaagaac
    21721 cccgtgtctg aattctctgt actctgcgct gttccagatg tctaaaaccg aatctctctt
    21781 gacgttaccg aatgccttaa tcggtcctgg ataagctcct ttgagcatcc ggcaacacgg
    21841 cactacctca ccggagaccc acaaataagc tgcttgcatg aacaggcatt ttgactcatc
    21901 gagcggatga agacggggga aagttatctt cagcccagac ctgtctccta tctcagcagc
    21961 ctgttcaaac aggggagcaa gaagtttttc gcccaatata cggaactcgc ctgctttgaa
    22021 gtcccttaga tggctgaacg tgactgtatc gagacccagt gatccggcca gccgtgcgaa
    22081 ggccggtatt tcacggacgt tttcgttctg aataacatag tttgcgacga gccagagcct
    22141 cttccgtccc gaaacatttc tgtactggac cgtgtgggcg atgttctgaa tcacctcatc
    22201 gaaattagcg ccaggtcgga tgctctcgta tgtaagcttg gttgcgccgt caatggaaaa
    22261 agaaagccaa ttcacaccca agtcaatcag gcgggaggca gcccttttat tcatcaaagt
    22321 accgtttgta gtaaacccta cctggccgtt ttcaggcatc ctctttttta actctccaat
    22381 gagttcgaat aaattctcat aaagcaaagg ttctcctata ccgaaagcag atactgaata
    22441 aaggaagggg ctggcgtcaa gaacgggttg tagaacttcc ggttttatgt cagacgcatc
    22501 ccagtcttca ggattgtgtc tcatgcacat gtgacaactg aggttgcagc gctgtgtcac
    22561 gggaaggcat acctgttcaa ggggaagatc acttttcccc ggcctgatta catgctgaga
    22621 cgacttcacc attaattgta caggagggtc ttttgaaaaa tcccataaag agatatgtag
    22681 catgctatat ccgataaatt ccaaatcccc tttccacacc ggtttcaggc gtacagggcg
    22741 catgctgtcc ttcacaatca gttcgtcctt ctcgaaggtc agagaagttt tctcacgggt
    22801 tatataattg agcgggatat cccagaatcc atgatgtcta ttcatatggg aaggtgagta
    22861 gatgggatac aaatcgaagc gtatgaagta tcttctggaa cccagaagcc gcctgtcgta
    22921 aatatgaagc gtcagctccg tatcttcttc tattatccac gggatttgaa catcaaagcg
    22981 cttatccatg taatttgatt cctggctcat ggcaccaagt aggccttgca gtcacaatca
    23041 gcgcattccg ccgggaaatc tctcctcacg gttcggcgcc ggaattcttg ataatccgga
    23101 cgcttccaga tctccaagag gggggtgcgg cgcacatcac caaatgatct gttcctggtt
    23161 agggtcttat aaccaggcgc catggcatga cacgggaaga cttcccccgg caacaacgct
    23221 accgcccgtt ccataaaaaa acagatttcc ttagggtttt tgcggagggg gggcgtgtcc
    23281 acattcatgt gtaacttctt cccctcctcc cgcgctttat gaaaaagctc ctggagcact
    23341 tcggccccaa agaccttcaa acctccggtg gcggtatcgg tgcaataact gaaggttacg
    23401 tgttcgactc cgagttgcgc cgccaaagat atgaatgccg ggatttcatg gagattttgc
    23461 tccaggatga caaaattcag cataatgcgc aaattattcc ggccggaagt tttttgcaac
    23521 ctggtacaac ggcgaatatt ctcagtgacc ctatcaaacc gggctccgac cctgatggct
    23581 tcgtagacct tcctggaagc cccatccatc gaaaaataca ggaaatcgat gccggtatcc
    23641 aggagacgag ctgcggtgtg ctcatcgagc aaagtggcat tggtggtgag acctacttcg
    23701 ccaccagctg ccatcctgtc tttgattctg gggatcaggc tgaaaatctt agggtaaagc
    23761 aatggctccc cttggccctg gagcaaggcc gacacaaagt ttgagccggc ttccagtaaa
    23821 ggctcaagag cctcgtcgga tatatccata tcaactattt cgtctgtggc ttgcctggcg
    23881 cacatggtac atttgagatt gcagcgatcg gtcaccggga gacaaatttg tttcaacgga
    23941 aggtccagat tttttatagc gattactaaa ggttgtgacc tcacttctat catatgcggc
    24001 ggctctttgg aattgtccca gagagaaaca tgcagcatgc aatatccatc ttcttcaata
    24061 agaccgcgcc acccctgttt gatctcaagc ggaactttct gattctccaa gctgataacc
    24121 cgtctgttga aatctaagac aaacgaggtg ctctcctgct ttatatactt tagcgggatg
    24181 tcccaccagc cataatgtct cctcatgtgg ccgggggcta ttaaagggta taaatcgagt
    24241 ctgataaagt ggtcgtccga accataaagg cgacggtcag agacagtgag cctgccgcga
    24301 gcgggtcctg tcaacaggta tggtatttct atatcacatg tcttattcac agtctaagct
    24361 cctctttcct tttcatcttc aggaattaca gccaaaaatc ccgatgtcac tacttgcaat
    24421 accgaatcgt caattttcct tttcctctgc aaggctgctc ttgcaggcat atatttccaa
    24481 aaacaaccca aaaacctcat gacgcctaat atcctgtaag gtcttgtaca ggacataagg
    24541 gttcttaaaa aagccctcaa gaaaagacgc ttgccgaagt tcttgaagta cgtgagtagc
    24601 gtgttgcgaa atacctgtga ttcacgcctg ggatcagcca atccatatgc gcttcccccg
    24661 taatggtata accccaactc cggtatgtgc acgacaacat gaccatgaag ccagcaccta
    24721 agccccaaat cgtggtcttc ccagttggaa ataaagcctt cgtcgaaaag atagtcaagg
    24781 tcgtctaccg ccgacctcct gatcatgaag cctgcgccgc acggagcaga gatgaagcgt
    24841 tcacccgcaa ggagcttctc attgtttgtg atcaaaccgt ctattatgaa ggtgtctgcg
    24901 taccgggttc tggagccctt agtttcaatc gtagacccga cgattgaaat gtgaggcgat
    24961 agctctatat gttctacaaa acgtcgcaca gctccctgtt ccaaaaccac atccgcattc
    25021 agcacgcata tgaattctcc tttgcatttc gagagcccaa aattgttccc cttgcaaaat
    25081 tcaaggtttt gttccatcac aaacagattc aagtggctga attcttcacg aattatctta
    25141 acagtgtgat catcagagtg gttgtcaatc acgacgacct cgatatttgg gtaatcctgc
    25201 gcagcaatgg aattcaaaca gtgccggaca tagccctcgc tgttataggt gacgatgagg
    25261 cagctcacaa gcggggcctc attatcctta acatattttc gtaaacgcat agaaacagaa
    25321 tagtacggta tgattacggg ctgggtatgg tggtgttgcc gatatccggc gacggtcagg
    25381 gttcctttca gggttgaaca caataaacct gctctttccg catcctttcc aataccgagc
    25441 acaagagagt aagtcttgcc gcgactgtcc cgtatcggtt cgaaccggaa aggttcgtta
    25501 aacttctgca atttaacacg tgacccgaac tccagcgtat gccttgcctt ccgcaacgca
    25561 acaggattat attcctcaaa gacgtgaagc gtgagctctg caggttccct ctcgccatac
    25621 gacctgacca gaagatcgaa tgacatcccg gataggttat cctccatact tgtgaaagtg
    25681 aatatgtatg aattccctcc tcttatgcct attctaagca aacgcccgag cggcacattg
    25741 tcgcaaaatt caagagttcc tcctcttata aaaggcgctg cttcaacctg tgtgaactcc
    25801 cggcacatat cgtgggtcat aacggtacgt ttggaaaccg gctttcaaaa gtgctctggt
    25861 ttatccactc tctattcctc agggtaagat gtcttcgtat gaaccacacc agagactccg
    25921 gtaaatgatc atatgtcaaa agatagaaat aatacaggat agattgccgg gtcagaccgt
    25981 tgatcatgaa aaagtctttt gtattctttg cgcttaagga aaaacgataa gaagataaat
    26041 tcttggcgtc agcccttatc cgatgtacac aaaggggttc ctgaaggcag gcgatacttc
    26101 cggaacgtct gagaaccttt ctcatgaaat gctcgtcggc cagcaaatag tctgcatcat
    26161 aacggaagct gtcctttcgg aacacgagag acgtatggtt gacaatgtca gctacgaaag
    26221 gatttcctat caggataaga acacccttgt cttcataaaa gaaatatgta ttagttccgc
    26281 aggcgacaa
//
LOCUS       DATKOU010000026         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1572511, whole genome shotgun sequence.
ACCESSION   DATKOU010000026 DATKOU010000000
VERSION     DATKOU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572511"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VMU21_01060"
     CDS             <1..863
                     /locus_tag="VMU21_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76140.1"
                     /translation="PNQDSSFYRELGIVGRDFSTFAWEGIVAHELLHKCLHETRPYEG
                     EQGSADRLYEEYLKKLSLSLRGADGKVEGVLENMRKDFENLPRYTHKKPLVGIVGEIF
                     VRSHRFSNEDLIRKVEGLGGEAWLAPVEEWIYYVNLMGLRKALIKKERSAIIELLLKD
                     FFQKRIEHRLARYFKGFLKTLNEPGTKELLRKASPYLHDSFEGEAVLSIGKSIDLIEK
                     GVSGIINAMPFGCMPGTVVTSLMRGISRDYGIPCISMPYDGTESPAMEIQLEAFMSQA
                     ARNTACKESH"
     gene            980..1075
                     /locus_tag="VMU21_01065"
     CDS             980..1075
                     /locus_tag="VMU21_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HUO76141.1"
                     /translation="MGSVVKWRKKKMSKHKHKKLLRKTKHQRRNK"
     gene            1111..1320
                     /locus_tag="VMU21_01070"
     CDS             1111..1320
                     /locus_tag="VMU21_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76142.1"
                     /translation="MNQEKHLKEKIALIEKELATITDLADELSDALKDVEDLKLELKG
                     LKVFLGRMHPEFKGHFPEILRKVKR"
     gene            complement(1317..1862)
                     /locus_tag="VMU21_01075"
     CDS             complement(1317..1862)
                     /locus_tag="VMU21_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76143.1"
                     /translation="MADDRVDCMYHVFRSLELAMSQTPEEAIRIALDKRFMDRGILES
                     GIVLNYDDRFQYGEDMLNSGKWGKEITSEIGPVTSIRGSRGRAKVKMVSKKNIIKYFK
                     KSFQLPLTDGDFVFFIKSPEKRVANEIVGHIGIIKWEENDMYLIHAAGRKNNGGEVSK
                     VLFLDYVSTMPFAGVRISRFP"
     gene            complement(2138..2794)
                     /gene="phoU"
                     /locus_tag="VMU21_01080"
     CDS             complement(2138..2794)
                     /gene="phoU"
                     /locus_tag="VMU21_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HUO76144.1"
                     /translation="MAIFDEELQSLKEKVLRMGLLVEAAIRDSIQSLIERDSELAKVV
                     IKRDHQINALDVEIDEECIRLIAIRQPKAGDLRFITTTMKITTDLERMGDLAEDICER
                     AIELNEEPPLKPYIDIPRMAQISQGMLIDVLDAFVKRDATTAYDIIKRDDEVDHLTVQ
                     IFNELLFMMIQDPRGVSRAVKITYIAKYLERIADHATNIAEMVVYMVEGRMIRHTESQ
                     "
     gene            complement(2794..>3085)
                     /locus_tag="VMU21_01085"
     CDS             complement(2794..>3085)
                     /locus_tag="VMU21_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HUO76145.1"
                     /translation="LVIARALAVEPEVLLFDEPTSALDPISTAKIEELIIDLKSSVSI
                     IIVTHNMQQAARISDWTGFMMLGELIEFGKTDKIFTTPSEKLTEEYITGRFG"
BASE COUNT          804 a          744 c          732 g          805 t
ORIGIN      
        1 caccgaatca ggattcctct ttttacaggg aacttggtat tgtgggaagg gacttttcca
       61 cttttgcgtg ggaagggatc gtggcacatg aactcctcca caaatgcctc cacgagacga
      121 gaccctatga gggggagcag ggaagcgcag acaggttata tgaagaatat ctgaaaaaac
      181 tctccctttc cttaagaggc gcggatggaa aggtggaggg agtactcgaa aatatgagga
      241 aggactttga aaatcttccg agatacaccc ataagaaacc ccttgtcggt attgtgggag
      301 agatctttgt gagaagccac aggttttcca acgaggatct catcaggaag gttgaaggcc
      361 tcggggggga ggcatggctc gccccggttg aggaatggat ctactatgtg aacctcatgg
      421 gtctcagaaa ggccttgatt aaaaaggagc ggtctgctat cattgaactt cttttgaaag
      481 acttcttcca gaaaagaatt gagcacagac tcgccaggta ttttaagggc ttccttaaaa
      541 ccctgaatga gcctggaaca aaggaattat tacgaaaggc ttcaccctat ctccatgact
      601 catttgaagg tgaggcggtc ctgagcatcg ggaagagcat tgacctcatt gaaaaagggg
      661 tttcgggcat catcaatgcc atgcccttcg ggtgcatgcc cggaactgtg gtgacgagcc
      721 tgatgcgcgg gataagcaga gattacggga ttccctgcat aagcatgcct tatgacggaa
      781 cagaatcccc tgccatggag atacagcttg aggccttcat gagccaggcc gcgcggaata
      841 cagcctgtaa agaaagccat tagctgtcag cggttcaact ctgcgaaagg cacagaaagg
      901 tgccgcgtca gttttctcgt tagtttatgc tgaatgctga tcgctggatg ctgaatacta
      961 taaaaaagga ggtggatgtg tggggagcgt cgtaaaatgg cgtaagaaaa agatgagcaa
     1021 gcataaacac aaaaaattgc tcaggaagac aaaacaccag agaaggaata aataattcta
     1081 ttccgtagtc tgcaatccgt agggggctga atgaaccagg agaagcattt aaaagaaaag
     1141 attgctctca tagaaaaaga gttggcaacg atcacggacc tggcagatga gttaagtgat
     1201 gccctaaagg atgtggagga cctgaagctg gagttaaagg gactgaaggt gtttctggga
     1261 aggatgcatc ctgagttcaa aggccacttc ccggagatac tccgaaaggt caagcgttag
     1321 ggaaacctgc ttatcctcac ccctgcaaag ggcattgtac tcacataatc aaggaataat
     1381 accttactga cctcgccgcc gttattcttt ctcccagccg cgtgaatgag gtacatgtcg
     1441 ttctcctccc attttatgat tccgatatgg cccacgattt cattcgcaac acgcttttcg
     1501 ggtgacttaa taaagaacac aaaatctcca tctgttaatg gtagttgaaa gcttttctta
     1561 aagtatttta ttatgttttt cttagagacc attttgactt ttgccctgcc cctcgatccc
     1621 cttatcgatg tcaccggccc gatctcactc gtaatctcct tcccccattt accgctattc
     1681 agcatgtcct ccccgtactg aaatctgtca tcataattga gcactatgcc actttccagt
     1741 atccccctgt ccataaatct cttatcaagc gcaatacgta ttgcctcttc gggggtctgg
     1801 ctcatagcaa gctcaaggct tctgaagacg tgatacatgc aatccacgcg atcatcagcc
     1861 actatcaccc tccttgtcac atatgtgccc aggggatcag ggtcgtaggg agtgcctacg
     1921 aactgttctg accaaaaagc gatcctctca ccaataggtt taggggatat ctgtttctga
     1981 aaagaagcaa tgtcggcatc attgaaagca aataggatac tgggaaagat gatcagacag
     2041 aagagcgcca gagtgtcata ccatttcgct cgaaagtaag gttcttttcg tacagaactt
     2101 tggcatttga gctttgttgc cctgcagatg tgcattgtca ctggctctct gtatgccgta
     2161 tcattctgcc ttccaccata tagaccacca tttccgcgat attggtggca tggtcagcaa
     2221 tcctttcgag gtactttgcg atataggtta tcttcacggc cctggaaact ccccttgggt
     2281 cctgaatcat catgaagagc agttcgttga atatttggac cgtgagatgg tccacttcat
     2341 cgtccctctt gataatatcg tatgctgtcg tggcatctct tttcacaaaa gcatccagga
     2401 catcgatgag catcccctga gaaatctgcg ccatccgcgg aatgtcgata tagggtttga
     2461 gcggcggctc ttcattgagc tcaatggccc tttcgcagat gtcctctgcc aggtcgccca
     2521 tcctttccag atctgtcgta atcttcatgg tcgtcgtgat aaatctcaga tcccctgcct
     2581 taggctgccg gatcgcgata agccgtatgc actcctcgtc tatctccaca tcaagggcat
     2641 tgatctgatg gtccctcttg ataaccactt tcgcaagttc gctgtccctc tctatgagag
     2701 attgtattga atcccttatg gcagcctcca ccaaaaggcc catccttaat accttctctt
     2761 taaggctttg cagctcttca tcaaaaattg ccatcagccg aacctccctg tgatatattc
     2821 ctcagtcagc ttttcagagg gggtagtgaa gatcttgtcg gtcttcccga attctatcag
     2881 ctcaccgagc atcataaatc ctgtccaatc agatatcctt gctgcctgct gcatattgtg
     2941 ggtaacgatg ataatgctca cactgctttt cagatcgatg ataagttcct cgatctttgc
     3001 ggtggatatg gggtcaaggg ccgaggtggg ctcatcaaag agcaggacct caggttccac
     3061 cgccagcgcc ctcgcaatga caagc
//
LOCUS       DATKOU010000027         7590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_805095, whole genome shotgun sequence.
ACCESSION   DATKOU010000027 DATKOU010000000
VERSION     DATKOU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805095"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1374
                     /gene="tolB"
                     /locus_tag="VMU21_01090"
     CDS             43..1374
                     /gene="tolB"
                     /locus_tag="VMU21_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HUO76146.1"
                     /translation="MKRTLTTQRPFRKKRGEDARIFIACVFLSILVLFVVHCLVYPVA
                     EAKEYIDITSPAFKKLPIAVSEFAGSSGKEISDIIRDDLDFTGIFLCLERNAFLETSS
                     QPFQQKNWTVIGAEAVVKGSVGGGGGKGLLASVSLYDVAEGKELFRKEYRTEGSLVRP
                     LAHVVANDIYKYFTGENSIFRTRIAFVGRKGGEDSLSLMDWDGYRINDLGIKGNVLLT
                     PRWSKDGNKLLYSSERYRQWGIYLLNFTTMTETKVFSSKGTNIAGDFFPNADNVIFSS
                     SVGGSPDLYMYSISTSKLTKLSSSRGIDVSPSVSPDGSQICFVSDRDGTPQIFLMGSD
                     GYAVRRLTFQGAYNTAPSWSPKGDKIVFSGRQGGKNHIFAINPDGSGLTQLTDRGNSE
                     DPSFSPDGRYIVFSSDMDGDRAIYIMRANGEAQKKITPKGIKAFGPRWSSN"
     gene            1392..1958
                     /gene="pal"
                     /locus_tag="VMU21_01095"
     CDS             1392..1958
                     /gene="pal"
                     /locus_tag="VMU21_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HUO76147.1"
                     /translation="MKRFLACMAIIGFAIFGCAEKQAVAPVPQPGELTPPSQPKEVEK
                     PKQVPVTEQVTEQQIAKVESGEVPSKMEEVSGMFKDIYFDYDKYEINENGRLVLRSVA
                     DYLTKNRAHKILIEGHCDDRGTSEYNLALGDKRAKSAKDFLLSLGVTSSRAEVISYGK
                     EKPLCSERTEECWAKNRRAHFVILKGKK"
     gene            1960..2838
                     /gene="ybgF"
                     /locus_tag="VMU21_01100"
     CDS             1960..2838
                     /gene="ybgF"
                     /locus_tag="VMU21_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HUO76148.1"
                     /translation="MVRLGFVLFLLMVGGCVSTSDFDAMKADISQLRRDSNELKKSVT
                     ALKEQTSGTVKEDSFHAIRESQASLYSQVAEQSKEVQVLRGRFDENKFFIDKALKESS
                     IERELLRSQISSLEKRIKDLNERLTRLSEPAVSTAQKPSAEGEDSPERVVPQKSPDDE
                     PAKAYDAAYTLLKDKQFKEAREKFNAFIKRFPKDGLAGNAQFWIGESYYAEKDFESAI
                     LAYETLIKNYPHNEKIPGALLKQGLSFAEIGDKKTAKVILDKLIEKYPDSREAALAKK
                     KKAELDKKPVKPQKNR"
     gene            2887..3924
                     /gene="moaA"
                     /locus_tag="VMU21_01105"
     CDS             2887..3924
                     /gene="moaA"
                     /locus_tag="VMU21_01105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HUO76149.1"
                     /translation="MKDPKLTDKFHRTIDYLRISVTDRCNLRCIYCMPSEGVKHIEHR
                     DILSYEEIARIVRVAAGLGVRKVRITGGEPLARKHITRLIASIREIPRIDDLSLTTNG
                     VLLEHYASELADAGLHRVNVSLDSLRSDRYREITRGGDISRVMKGIEAAEEAGLMPVK
                     INMVPVRGFNGDEIGEFARITRDTPYHVRFIEFMPIGGRELWSPEKYIATDEIKATVE
                     RAGPLFPVRVRRTGPARYFRFADAPGVIGFISALTHHFCEDCNRLRITSDGKLRPCLF
                     SETEIDLKPALRGASTPSDKEIERLLRLAIEIKPERHMLDESRELLPDGSTLSLTSLP
                     GPLKPMSRIGG"
     gene            3925..4449
                     /gene="moaC"
                     /locus_tag="VMU21_01110"
     CDS             3925..4449
                     /gene="moaC"
                     /locus_tag="VMU21_01110"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HUO76150.1"
                     /translation="MGLTHLDKRGKAKMVDVSAKPATEREAVARGSVSMKPATMKLVQ
                     DGAMPKGDVMAAARIAGIMAAKKTADLIPLCHPLAITSVTVDMSIDEKKSRMDIVAKA
                     KTVGQTGVEMEALTAVSVAALTIYDMCKAVDREMTISDIMLMEKRGGKSGEFRKSAAS
                     PVSPKAAVRKSQKK"
     gene            4628..7171
                     /gene="mgtA"
                     /locus_tag="VMU21_01115"
     CDS             4628..7171
                     /gene="mgtA"
                     /locus_tag="VMU21_01115"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HUO76151.1"
                     /translation="MLPYSSNQGSAFWQKDITTLLKELDAALVGLSESEASARLAAFG
                     PNLLHPVRKRILLLQFLEKFRNPLVLILLVASVVLALTGDVTSFLIITVIVLISVTLD
                     FIQEHRAGQAADRLRKSVAVHVQALRDGNPREIPVAELVPGDIILLAAGDLVPGDGRI
                     IESRDFFVNQALLTGEPYPVEKTPGDMAEESDILAANNSVLLGTSVISGSAKVLVCRT
                     GANTVLGDIADTLVAKPPPTAFEQGTRNFGLLIMRFTVLLVLFVLLVNAFFHRPWLES
                     FLFAVALAVGLTPELLPMVVSVTLARGAQRMAAKKVIMKRLAAIQNLGSMDILCTDKT
                     GTLTEARIRLERHVDCRGRDSKRVLELAYLNSYFETGLKSPLDDAILEHIEIDIAGWR
                     KIDEVPFDFERRRVSVLVDNGQTRLLVVKGAPEDIVRLCTSCEEDSASSSPLDSTTLS
                     SVQNLFERLGREGFRVLGVAWREVEADHPHAVVGDETGLVFAGFAAFLDPPKESARDA
                     LSRLAASGVSVKIVTGDNELVTEHICNELGLPIVGILTGAEIHQMDDQALSVQVERAN
                     LFCRVTPIQKNRVILALKKRGHAIGYLGDGINDAPSLHSADIGISVDSAVDVAKDAAE
                     MILLEHDLGVLYEGVMEGRRTFGNVMKYIMMGTSSNFGNMFSMAGGSLFLPFIPMLPV
                     QILLNNMLYDISEVPIPLDNVDEEYLIRPRQWDIGFVRKFMLAIGPVSSLFDFLTFFV
                     MLGVFHAGEKLFHTGWFIESLATQVLVIFVIRTHKNPFRSRPHPLLTASSLIVVACAI
                     ALPFTPLAAYLGFEPLPPLFFLVLTGMVIIYLLIVEQVKQWFFNRYLSA"
BASE COUNT         1939 a         1881 c         2010 g         1760 t
ORIGIN      
        1 tgccccaccc catgaaatgg agataggggt caggttctac ccatgaaaag aaccctcacc
       61 actcagcgcc ccttccgtaa aaagaggggg gaggatgcac gcatcttcat cgcctgtgtg
      121 tttctttcca ttcttgtttt gttcgttgtt cactgtttag tgtaccctgt tgctgaagcc
      181 aaggaataca ttgatatcac ctctcctgcg ttcaaaaaat tgcccatcgc ggtttctgaa
      241 ttcgcgggat catccgggaa ggaaatttct gatattatcc gggatgacct ggattttacc
      301 ggaatcttcc tctgccttga gagaaatgcc tttctggaga cttcctcaca gccttttcag
      361 caaaagaact ggaccgtgat cggcgctgag gccgtcgtga agggctctgt ggggggaggg
      421 ggaggaaaag gtctgctggc atctgtttcc ctttacgatg tggcagaagg gaaagagctt
      481 ttcaggaaag aataccggac agagggatct cttgtgagac ccctggcaca cgtagtggcg
      541 aatgatattt acaaatattt cactggtgaa aatagtatct tcaggacacg aatcgcattc
      601 gttggaagga aagggggaga agattcgctc tctctcatgg actgggacgg atacaggata
      661 aacgacctcg ggataaaggg aaacgtgctg ctcactcccc gttggtcaaa ggatggcaac
      721 aaactcctct attcatcgga aagatataga caatggggaa tctacctgct aaactttacc
      781 acgatgaccg aaacaaaggt tttctcttcc aagggcacca atattgcagg tgattttttc
      841 cccaatgcgg acaacgttat cttctcctca tctgttggag ggagccccga tttatacatg
      901 tatagcatat caacgtcaaa gctcacaaaa ctctcctcct cgcggggaat cgacgtatcg
      961 ccttccgttt cccctgatgg cagtcaaata tgctttgttt ccgacaggga tggaacccct
     1021 cagattttcc taatgggcag cgacgggtat gcagtgagac gcctcacctt tcagggggct
     1081 tacaacaccg cgccttcctg gtcgccgaag ggagacaaga tcgtattttc aggaagacag
     1141 ggagggaaaa atcacatctt tgccataaac cccgacggtt ccggattgac ccaactcacc
     1201 gacaggggga acagtgagga cccttccttt tctccggacg gaaggtatat agtattttca
     1261 tctgacatgg atggcgaccg tgccatctac atcatgagag cgaacggaga ggcacaaaaa
     1321 aagataacac ctaaaggtat aaaggccttc ggtccccgat ggtcatcaaa ttaacagagg
     1381 aggtcttctc aatgaaaagg ttcttggcat gtatggcaat cataggattt gcgatattcg
     1441 ggtgcgctga gaagcaggcg gtggcgccag taccgcaacc cggagaattg acgccgcctt
     1501 cccagccaaa ggaagtggaa aaaccgaaac aagtccctgt caccgaacag gttaccgaac
     1561 agcagattgc caaggtggaa tccggtgaag tgccttcaaa gatggaagag gtttcgggga
     1621 tgtttaagga catatatttc gactatgaca aatatgaaat aaacgagaac ggaagacttg
     1681 tgttaaggtc agtggcagat tatctcacca agaaccgtgc ccataagata ctcattgaag
     1741 gccattgcga tgaccggggg acatcagaat ataacctcgc cctgggagac aaaagggcaa
     1801 aatctgcgaa ggactttctc ctttccctgg gcgtaacttc atcccgcgca gaggttataa
     1861 gctacggcaa agaaaagccg ctttgcagtg aacgcacaga agagtgctgg gcaaagaaca
     1921 gaagggcaca cttcgtgatt ctcaagggga agaaataaca tggtgcgact gggttttgtg
     1981 ctctttctcc ttatggttgg cggctgcgtc tcgacatcgg atttcgatgc aatgaaagcg
     2041 gatatcagcc aactgagaag ggattccaac gaactcaaga aaagtgtaac cgcactgaag
     2101 gaacagacat cgggaactgt gaaggaggat tcctttcacg caatcaggga aagtcaggca
     2161 tccctttatt cccaggtggc ggagcagtca aaggaggttc aggttctccg ggggaggttt
     2221 gatgaaaaca agttctttat tgataaggca ttgaaggaaa gttccattga gcgtgaactt
     2281 ctgcgctccc agatcagcag tctcgaaaaa cgcataaagg accttaatga acgcctcacc
     2341 aggctgagtg agccagccgt ctccacagct cagaaaccat cggcagaggg agaagattcc
     2401 cccgagaggg tggtgccgca gaaaagccct gatgatgaac ctgcaaaggc atacgatgca
     2461 gcctataccc tcctcaaaga caaacaattc aaagaggcga gggaaaagtt taacgcgttc
     2521 atcaagaggt ttcctaaaga tggcctcgca gggaacgcgc agttctggat aggagaatcc
     2581 tattacgcgg agaaggattt tgaaagcgct attctggcgt acgagacgct catcaagaat
     2641 tatccgcata atgaaaagat tcccggcgca ctcctgaagc agggcctctc ttttgcggag
     2701 ataggggaca agaagaccgc aaaggtcatt ctggataagc tcatcgaaaa ataccctgat
     2761 tccagggagg cagcgcttgc gaaaaagaaa aaagccgaac ttgacaagaa accggtgaaa
     2821 ccacagaaga acagatagca agaaaggtcg gggctgaggt tgattattcc cctgatctta
     2881 acctttatga aagaccccaa actcacagat aaattccacc gcaccataga ttacctgcgt
     2941 atctctgtta ccgacaggtg caatctcagg tgtatctatt gcatgccttc tgagggcgtt
     3001 aagcacatcg aacacaggga tatcttgtct tatgaggaaa tcgcaaggat cgtgagagtt
     3061 gccgctggcc tcggggtgag aaaggtcagg attacagggg gagaacctct cgcacggaaa
     3121 catattaccc gccttattgc atccatcaga gagatccccc ggatagatga cctgagcctc
     3181 accaccaatg gagtgctcct ggaacattat gcaagtgaac tagcggatgc agggctgcat
     3241 agggtgaatg tgagcctgga ttccctcagg tctgacaggt accgggagat cacgcgtgga
     3301 ggggacatca gccgggtaat gaaagggata gaggcagcgg aagaggccgg gctgatgccc
     3361 gtaaaaatta atatggtgcc cgtcagggga tttaacggag atgagatcgg ggagtttgcg
     3421 aggatcacgc gggatactcc ctatcatgtg aggttcatcg aatttatgcc cattggagga
     3481 agggagctgt ggagccccga gaaatacatt gcaactgatg agataaaagc cactgtggaa
     3541 cgagccggtc cccttttccc tgttcgggtg cgcagaaccg gaccagcccg atatttcagg
     3601 ttcgcagacg ctccgggtgt cataggcttt atcagtgcat tgacacacca cttttgcgaa
     3661 gattgcaacc gcctccgcat cacttctgat ggaaagttaa gaccctgcct cttctctgag
     3721 acagaaatcg atttaaaacc cgcgcttcgg ggcgcctcga ccccttctga taaagagatc
     3781 gagcgcctgc tcaggcttgc catagagata aaaccggaaa gacacatgct cgatgagagc
     3841 agagagctgc tgcccgatgg ttcaacgttg tccctcacct ccctcccggg accactaaaa
     3901 cccatgtcga ggataggagg ataaatgggg ctcactcacc tcgacaaaag gggaaaggca
     3961 aagatggtgg atgtttcggc aaaacccgca acagaaaggg aagctgtggc aagggggagt
     4021 gtctccatga agccggcaac aatgaaactc gtacaggacg gggcaatgcc aaagggtgac
     4081 gtgatggcag cagcgagaat tgccggaatc atggccgcaa aaaaaacagc agaccttatc
     4141 cccctttgtc atcccctggc cataacctcc gtaacagttg atatgtctat cgatgagaag
     4201 aagagcagga tggatattgt ggcaaaggcc aagaccgtgg ggcagacagg ggtggagatg
     4261 gaagcattaa ctgccgtgtc cgtggcagcg ctgaccattt acgatatgtg taaggcagtg
     4321 gacagggaga tgaccatttc cgatatcatg ctcatggaga aacgcggagg gaaaagcgga
     4381 gaattcagga aaagcgcggc ctctccggtt tcaccgaaag ctgcagtaag aaaatctcag
     4441 aaaaagtaag cacacgaacg aatgcttctg ataatccaca ccgcggtagt ctcaatggag
     4501 acgcagattt cctcaagaag cattcagcca tgaaactcat ctcagaccaa gccctgctgt
     4561 agttcgcacc ttaggaacct gatataatgt ggcagatagc tttgcccgta tcttaggagg
     4621 cggaacaatg ctgccttatt cttcaaacca aggatcagca ttctggcaaa aggacataac
     4681 gaccctcctg aaagaacttg acgcggctct tgtgggtctg tcggagagcg aagcctctgc
     4741 acgtcttgcc gcgttcggtc ccaatctgct gcatccggta agaaagagaa ttcttcttct
     4801 gcagttcctg gagaagtttc gtaacccgct ggttcttatt ctccttgttg ccagtgtcgt
     4861 gttagccctg accggcgacg ttaccagttt cttaatcatc accgttatcg tgctcatcag
     4921 cgtgacactc gattttattc aggagcatag ggcaggacag gcagccgacc ggttgcgtaa
     4981 gtccgtggcg gtacatgtac aggcgctgcg cgacggcaac cctcgcgaga tacctgtggc
     5041 agagctcgta cctggagata tcattctcct tgcggctggt gacctggttc ctggcgatgg
     5101 acgcataatc gagtcaaggg acttctttgt aaaccaggcc cttcttaccg gagaacccta
     5161 cccggtcgag aaaacacctg gcgacatggc agaggagtca gatatccttg ccgctaacaa
     5221 ctccgtactc ctggggactt cggtaatcag cggtagcgcc aaagtgctgg tttgccgtac
     5281 cggagcgaat accgtgcttg gtgatattgc cgataccctg gttgccaagc cccctccgac
     5341 cgcgtttgag cagggcacac gcaatttcgg gttgctgatt atgcgcttta ccgtgcttct
     5401 cgtattgttt gttcttcttg tcaatgcctt ctttcacagg ccatggctcg aatcttttct
     5461 ctttgcagtg gcccttgccg tgggcctcac tcccgaactt ctccctatgg tcgtctcagt
     5521 gactcttgcc cgtggtgcac agcgtatggc tgcaaagaag gtgattatga agcggcttgc
     5581 cgcgattcag aatctgggaa gcatggacat cctttgcacc gacaagacag gtacactgac
     5641 cgaggcacgc attcgcctgg agcgccacgt ggactgtcgc gggcgagaca gcaagcgcgt
     5701 gctggagctt gcatacctga acagttattt tgaaacaggc ctgaaaagtc ccctggacga
     5761 cgcaattctt gagcatatcg agattgacat agcgggctgg cgcaagatcg atgaggttcc
     5821 ttttgacttt gaacggcgcc gggtctcggt gcttgttgac aatggccaaa caaggctcct
     5881 cgtagttaaa ggagccccgg aggacatcgt gcgtctctgc accagctgtg aggaagacag
     5941 tgcgtcatcg tctccattgg atagcacaac tctctcctcc gtccagaacc tttttgaaag
     6001 gctgggacgt gaaggctttc gtgtattggg cgtggcatgg cgggaagtgg aagcggatca
     6061 tcctcacgcg gttgttggcg atgaaacagg gcttgtcttt gccgggttcg cagcgttcct
     6121 tgatcctccc aaggagagcg cgagggacgc gttgagcagg cttgccgcca gcggtgtctc
     6181 tgtcaagatt gtaaccggcg acaacgagct ggttacggag catatctgca acgaactagg
     6241 attgccgatc gtcgggattc tgaccggtgc ggagattcat cagatggatg atcaagccct
     6301 ctcggtgcaa gtggagaggg cgaacctgtt ttgccgtgtc accccgatac agaagaaccg
     6361 ggtcattctc gccctgaaaa agagggggca cgcgataggg taccttggtg acggcatcaa
     6421 tgacgccccg tcacttcatt ccgccgatat cggcatctct gtggacagcg cggtggatgt
     6481 cgcaaaagat gcggctgaga tgatcctgct ggaacatgac ctgggagttt tgtacgaagg
     6541 agtgatggag ggacgacgca cgttcggcaa tgtcatgaaa tacataatga tgggaaccag
     6601 ttccaatttc gggaacatgt tcagcatggc cggaggctct ctcttccttc ccttcattcc
     6661 catgctcccg gtccagatat tgctcaacaa catgctctac gatatctccg aagttcccat
     6721 tccattagac aacgttgatg aggaatacct gatccgtccc cggcagtggg acataggctt
     6781 tgtccgcaag ttcatgctgg ccattggccc tgtcagttct ctctttgatt tcctcacgtt
     6841 cttcgtgatg ctgggcgtct ttcacgcggg tgaaaaactc ttccacaccg gctggttcat
     6901 agagtccctg gctacccagg tgcttgttat ctttgtcatc cgcacccaca aaaatccctt
     6961 cagaagccgt ccccatccat tgttgaccgc atcttccctc atcgtggtgg catgcgccat
     7021 cgctcttccc tttacccctt tggctgccta tctcggcttt gagcccctcc ctcctttatt
     7081 ctttcttgtt ttgacaggca tggtgataat ctaccttctg atcgtggaac aggtaaaaca
     7141 gtggttcttc aaccgatacc tgtcagcctg acttttctcc tctctagccc tgctgaataa
     7201 aggtctgtgt tttgccgttg cgagcttggt gaatttccct tccacgtgag tgccatgact
     7261 tgcgattgtc tgtccacctg ctacacctcg gttatgcggg catgcaagtg tcgtgaactg
     7321 tatcccgtac tacgcacatc tcaaccgaga tgaaaaatca tgccagatgt cgtctctggc
     7381 tcgccgcctt atgaagtaca atgtcactta cttttggtaa tcaatacgca agggtcttat
     7441 tcctctgaaa agatagttat gtcctgaagt acgcaggtcc tctaacgaga gaatgaggta
     7501 gccattcgat ctagagtcca ccgacgaaca acacttatca gacattgaaa ctggtgcgga
     7561 ttttcaagag tgactaccta gtgaagcata
//
LOCUS       DATKOU010000028         4152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4654401, whole genome shotgun sequence.
ACCESSION   DATKOU010000028 DATKOU010000000
VERSION     DATKOU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4654401"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..435
                     /locus_tag="VMU21_01120"
     CDS             199..435
                     /locus_tag="VMU21_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HUO76152.1"
                     /translation="MTIAKATIKGHVIIPAELRKKYNIKKGSKVCIYEGKGGVIIIKP
                     IPEDPIGESRGMLKGKPSLLKTLMRDRREEADRG"
     gene            428..829
                     /locus_tag="VMU21_01125"
     CDS             428..829
                     /locus_tag="VMU21_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO76153.1"
                     /translation="MDSAYVLDSYALIAHFEDDAGGEKVRKILRAAHEGKTRLYLSVI
                     NLGEIYYNIVRERGVEKADQTMFMIEQLPIGIIDADKIITLEAAGLKAQYSIAYADCF
                     AAALGILKKARVVTGDPEFKKIGEKVRLEWI"
     gene            842..2947
                     /locus_tag="VMU21_01130"
     CDS             842..2947
                     /locus_tag="VMU21_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HUO76154.1"
                     /translation="MVERLKRIRQEILGVASIFGSIYIALSLATYSKWDPSLFVFSTL
                     PVQNYGGIVGSYLADFMVSTAGVVAVVIPIALVIYGIKEVLGKEGHWIYLLGFILFIL
                     SASMLASLVATTFHITLVNNLGGIVGSLLSDFAVGLLSIPGAYIFSLALFLSSLILLS
                     PVSLTSLAIRGPEKKKDEVEPVEEEGPIILGEPFTDHASMTMDTITHDIPITVHDREI
                     PRKTPPEDRSLFRLEKPVRRGEYELPGVEFLNPPVPLSSRPTREEIMSSSQLLERKLR
                     DFDVEGKVIHVHPGPVVTMYEFEPAPGVKINRVVALSDDLALSIKAQSVRAFPLPDKA
                     AIGLEVPNKNREMVYIREIISSENFQKSYSKLTLALGKDIFGNTIVADLLKMPHLLVA
                     GATGSGKSVSINSMVMSILYKATPAEVRMLMIDPKLLELSSYENIPHLISPVITAPKE
                     AAEALRKMVFEMERRYRLLAEKGVRNIDAFNRMVPDEELLPYIVIFIDELADLMFTSA
                     NEVEDSIARLAQMARASGIHLILATQRPSVDVITGVIKANFPARIAFQVTSRIDSRTI
                     IDSQGAEQLLGKGDMLLMLPGQRIERVHGALVTEEEVRAVTGFITSQGAPDYSLFETI
                     ASEAAGQEEASDERDEMYQKVLEFGEYTGEVSISSIQRRFKIGYNRAARVMELLEEDG
                     YVGPPKGAGKPRDFLRKRR"
     gene            3165..>4152
                     /locus_tag="VMU21_01135"
     CDS             3165..>4152
                     /locus_tag="VMU21_01135"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
                     /protein_id="HUO76155.1"
                     /translation="MRTKEVVEALQKTANLTRITPEILKENPSVLPMLRMVTAPPLAR
                     DRLMGLAYVNKSLIESMEGKKDKPPRVPSRMTKSKLDEELGRICDVISELADRDLFPW
                     LDSKQRPDQTDIDRSAAVVADRLCGAASDPIIRNAQEKRQLEALKRWLRKRGYNYIGT
                     DAARNPIEMSPGTFTFRLNLPAGKKSSPVNIPIDCVIKPLSYDKTSMPILIEAKSAGD
                     ATNTNKRRKEEAQKYSQLKERYGTGVSFILLLCGYFEPGYLGYEASEGIDWIWEHRLD
                     DLDQILNGKIKKKVQAVKEDTEPYVVSDDAAEAERFIRQKQIDDSKSAEERNR"
BASE COUNT         1142 a          926 c         1095 g          989 t
ORIGIN      
        1 tgctgcacct ccattctctc ttgggtgatt gtgctatttg atatcatgat actatggtac
       61 taccttagtt gtcaagagag ccctcctgga ggagcgtcac cgactaggcc aatacccagg
      121 gggcgaatca ttgaacagag atagacaaag tgagtagttt aaggtaaagt gttataacaa
      181 agtggaagga ggtataacat gactattgca aaggctacta ttaagggaca cgtgataata
      241 cccgccgaac tgaggaagaa atataatatc aagaagggga gcaaggtctg catctatgag
      301 ggaaagggag gcgtgattat aataaaaccg attcctgaag accccatcgg ggaatcccgg
      361 ggaatgctca aaggcaagcc ctccctgctc aaaaccctga tgagagacag acgcgaggag
      421 gcggaccgtg gatagcgctt acgtgcttga cagttatgcc ctcatcgctc actttgaaga
      481 cgatgcggga ggggagaagg tgagaaagat cctcagggct gctcacgagg gaaaaacaag
      541 gctgtatctc tctgtaatca atcttggtga aatatattac aatattgtga gggagagggg
      601 agttgaaaaa gctgatcaga caatgtttat gatagagcag ctgcccatag gcattattga
      661 cgctgataag attattacgc ttgaagctgc cgggctgaag gcccaatact ccattgcata
      721 tgcagactgt tttgccgctg cccttggcat cctgaaaaag gcaagagtgg taaccgggga
      781 cccggagttt aagaaaatag gggaaaaggt aagattagaa tggatttgag aaacaggcat
      841 catggttgag cgtctaaaga ggataaggca ggaaatactg ggggttgcct ctattttcgg
      901 gagcatctac attgccctga gccttgcgac atactccaaa tgggatcctt ccctctttgt
      961 gttttccact ctccctgtgc aaaattacgg aggcattgtt ggttcatacc ttgctgattt
     1021 catggtctct acagccggcg tagtggctgt ggtcataccc attgctctgg tgatctacgg
     1081 gataaaggag gtgctgggta aggaaggcca ttggatctat ctcctgggct ttatcctctt
     1141 catcctttcg gcttccatgc tcgcttctct agtcgctaca acctttcata tcactctggt
     1201 gaacaactta ggcgggattg tgggttctct tctttctgat tttgcggtag ggctgctctc
     1261 aattcccgga gcatatatct tttcattggc actcttcctt tcttctctta ttctcctgag
     1321 ccctgtctcc ttaacttcgc ttgccattcg tggacctgag aagaaaaagg acgaagtgga
     1381 gccagtggaa gaggagggac cgatcatact gggagagcct tttaccgatc atgcaagtat
     1441 gactatggac acaattaccc atgatattcc catcactgtc cacgaccggg agatacccag
     1501 gaaaactcct cctgaagacc ggagtctttt ccgtcttgag aagcctgtgc gcagaggtga
     1561 gtatgaattg cccggcgttg aattcctgaa cccgcctgtc cccctctcat ccaggccgac
     1621 gagggaggag atcatgtcca gctctcagtt gcttgagcga aaattgaggg attttgatgt
     1681 ggaagggaaa gtaatccatg tccatcctgg tccggtggtg accatgtatg aatttgagcc
     1741 cgcacccggg gtcaagataa accgtgtggt tgccctttcg gatgatctgg ccctttccat
     1801 aaaggcgcag agcgtgaggg cgttcccatt gccggacaag gcagctatag ggctggaagt
     1861 tcccaacaaa aaccgggaga tggtatatat ccgggagatc atctcatcag agaacttcca
     1921 gaagagctac tcaaagctca cgcttgcctt gggaaaagat atattcggca ataccattgt
     1981 tgccgatctt ctgaagatgc cccacctcct tgtggcgggt gcaacgggtt cggggaagag
     2041 tgtctccata aattccatgg tcatgagtat tctctataag gcaactccgg cggaggtgag
     2101 aatgctgatg atagacccta aactcctgga gctctcttcc tatgaaaata ttccccacct
     2161 tatctctccg gtaatcactg cccccaagga agcggcagag gctctcagga agatggtatt
     2221 cgagatggag aggagataca ggctccttgc ggagaagggg gtgaggaaca tagatgcctt
     2281 caacaggatg gttcctgatg aggaactgct tccctatatc gtgatattta ttgacgaact
     2341 cgctgacctg atgtttacct ctgccaatga ggtggaggac tccatcgcgc gccttgccca
     2401 gatggcaagg gcatcgggaa tacacctcat tctcgccaca cagaggccgt ccgttgatgt
     2461 gatcacaggg gtcatcaagg caaatttccc ggccagaatc gcatttcagg tgacctcgcg
     2521 tatagactca aggaccatca ttgacagcca gggtgctgaa cagctcctgg gaaaagggga
     2581 tatgctcctc atgctccccg gccagaggat cgagagggtg cacggcgccc tggttaccga
     2641 ggaagaggtc agggcagtaa ccggattcat cacttcgcag ggagcacctg attattcctt
     2701 gttcgagacc attgcttctg aagcagcggg gcaggaagag gcatccgatg aacgggacga
     2761 gatgtaccag aaggtcctgg agttcggtga atacacagga gaggtatcca tatcatccat
     2821 tcagcggaga tttaagatcg gctataacag ggcagcgagg gttatggagc ttttggaaga
     2881 ggatggttat gtgggaccac caaaaggggc aggaaaaccc agggatttct tgaggaaaag
     2941 gagatgaatg gatatgacta gaactttttg cagacgatca gttttgcggg gaatcatttt
     3001 ttgttaaagt agaactggat gtttaaatta attcaaaagg ggaaaagatg tcgataaatg
     3061 cagataagcc gcatctgtgg aaagcggacg tcgagacgtc aatcgacttc tataatgatt
     3121 agtttctgcg cttcgcaccg gtaacttaca gaaagcaacg tgttttgcga acgaaggaag
     3181 tagttgaggc cctccaaaaa acagcaaacc ttaccagaat tacgcctgaa atcctaaaag
     3241 aaaacccttc tgttctgccg atgctacgga tggtaaccgc cccacctttg gcaagggaca
     3301 gactgatggg attggcttat gtgaataaga gccttattga atcaatggag ggcaagaaag
     3361 acaaaccgcc gcgagttcct tccagaatga caaagagcaa acttgacgag gaattgggca
     3421 gaatctgtga tgtaatatca gaactagccg accgtgatct cttcccttgg ctggattcca
     3481 aacagcgacc ggatcaaaca gacattgaca ggtctgctgc tgttgttgcc gatcgtcttt
     3541 gcggagcagc atctgatcct attatccgca atgctcagga gaaacggcag cttgaagcat
     3601 tgaaacggtg gctaaggaaa cgaggttata actacatcgg cacggatgct gcacgtaatc
     3661 cgattgaaat gtctcccggc acatttacct ttcgtcttaa tctccccgcc ggaaagaaat
     3721 cttccccggt caatattccg atagattgtg taataaagcc tttatcatac gacaagacgt
     3781 caatgccgat tcttattgag gcaaaatcgg ctggcgacgc tactaatacg aacaaaagaa
     3841 ggaaagaaga ggcacagaag tattcccagt tgaaagaacg gtacggcacg ggagtttcgt
     3901 ttatcctatt gctctgcggc tattttgagc caggctattt gggatatgag gcatccgagg
     3961 gaatcgactg gatttgggaa catcgactgg acgacctcga ccaaatacta aacggaaaaa
     4021 ttaaaaaaaa agtccaggca gtaaaagaag acactgagcc gtacgtcgta tccgacgatg
     4081 ctgcggaggc ggaacgtttt atccgccaaa aacaaattga cgattccaaa tcagcagaag
     4141 aaagaaacag ac
//
LOCUS       DATKOU010000029         3379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3115036, whole genome shotgun sequence.
ACCESSION   DATKOU010000029 DATKOU010000000
VERSION     DATKOU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115036"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1353)
                     /gene="hisS"
                     /locus_tag="VMU21_01140"
     CDS             complement(85..1353)
                     /gene="hisS"
                     /locus_tag="VMU21_01140"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HUO76156.1"
                     /translation="MSSSQRYNALKGVQDILPPDIYLWQRVESVARKIFANYGFQEIK
                     APIIESTTLFTRSIGETTDIVEKEMYTFPDKAGRSITLRPEGTAPVVRAYVEHHLHTL
                     PPPQKFFYSGPMFRYERPQKGRFRQFYQIGVEAFGVATAELDGEVIAMLKVLLENLGL
                     RELNFEVNSIGCEECRPSYRSSLQDFFSSRLDHLCPDCKRRYEFNPLRILDCKVERCI
                     ETRKGAPKVTDFLCGECRDHFEGVLSILRLLQIPHLVNPDMVRGLDYYTRTTFEVTTE
                     HLGSQKAVAAGGRYDRLVEEFGGPFTPAIGFAIGMERLTALLKETGGTEVPSPQVFMA
                     SIGKRATQEGLLMAERFRAKGLWVEVGYEGSLRSQLRRADKLASHYVFIIGDDELSSG
                     KMQWKNLRDGSQGEVEITQADEFVSSRLIP"
     gene            complement(1350..1829)
                     /locus_tag="VMU21_01145"
     CDS             complement(1350..1829)
                     /locus_tag="VMU21_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HUO76157.1"
                     /translation="MRITPLDIQQKQFPMKFRGFDVEEVYAFLEIVREEMEDLLRENA
                     SLKEQIHRAENQIKEYRDMETTLRETLMTAQQMVEEYKTNARKEAELLIREAEIKADG
                     VMKEAQDKVVKIHEDIVDLKGIRRHFKEELKRLIESHMRMLEFDKEREGEEAGDISS"
     gene            complement(1826..2140)
                     /locus_tag="VMU21_01150"
     CDS             complement(1826..2140)
                     /locus_tag="VMU21_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HUO76158.1"
                     /translation="MFVLGNLILAIANILDIVLSLYMWVVIIAALISWVNPDPYNPIV
                     RFLHSVTEPVFRPIRRLIGFRLGPIDISPLVVILAIMFIQRFLIHSLVELGYKIKGGA
                     FI"
     gene            complement(2140..2928)
                     /gene="proC"
                     /locus_tag="VMU21_01155"
     CDS             complement(2140..2928)
                     /gene="proC"
                     /locus_tag="VMU21_01155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HUO76159.1"
                     /translation="MIGFIGGGNMAEALIKGMTSRGMSSIVVSEPSEERRKYLEQSYG
                     ITTTPHNREVASSCSLIILAIKPQNMSAVLDEVRDVITAEKTVVSIAAGITLAFLQSR
                     LATKRLVRVMPNTPALVQEGMSVLSLCECSSDMEIAKVREIFMSAGRVLTLPEAYMNA
                     VTALSGSGPAFFALFAEAMIGAGVAMGLTRDNASELAIQTLIGTAKLLETGMHPERLR
                     EMVTSPGGTTAAGLKVFQDKKLGSLVRDALEAALRRAEELGRRE"
     gene            complement(3001..>3379)
                     /locus_tag="VMU21_01160"
     CDS             complement(3001..>3379)
                     /locus_tag="VMU21_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HUO76160.1"
                     /translation="AELAEELNSQAEKIGRVQEVLLEVKLSPEETKHGVARAELLQFL
                     KMAQALKHLRIMGLMTIPPFFEHPEEARPYFRKLRELRDEAGSAGHPLPELSMGMSND
                     FEVAIEEGATMVRIGTAIFGERK"
BASE COUNT          757 a          959 c          776 g          887 t
ORIGIN      
        1 gatgtcaact ttgcaacaga atcgctggca ggattaaatc agaagggtgg gagaatagcg
       61 ttggaatatg cagagggcta tacttcaagg tatcagacgt gaagaaacaa actcgtcggc
      121 ctgggttatc tccacctcac cctgggagcc gtccctcaga ttcttccact gcatctttcc
      181 tgaggaaagt tcgtcgtctc cgataataaa gacatagtga gaagcgagtt tgtcagccct
      241 tcggagttgg ctcctcagtg acccttcgta ccctacctct acccataacc cttttgccct
      301 gaatcgttca gccatgagca gcccttcctg agtagccctt tttccgatgc tcgccatgaa
      361 cacctgaggt gagggcactt cagtgcctcc cgtttccttg agcagagcgg tgagcctttc
      421 catcccaatg gcaaacccta tggcgggtgt aaaggggcct ccaaactctt ccaccagtct
      481 gtcgtacctt ccccctgctg ccaccgcttt ttgcgaaccg agatgttcag tggtgacttc
      541 gaaggttgtc cttgtgtagt agtcgagccc ccgtaccata tcgggattta ccaagtgggg
      601 gatctggagg agtcttaata ttgaaagcac tccctcaaaa tggtccctgc attctccaca
      661 gagaaaatct gtcaccttgg gggcaccctt cctcgtctca atacaccgtt caactttaca
      721 gtccagtatc ctgaggggat tgaattcata tctccttttg cagtcaggac agaggtgatc
      781 aagtctggaa gagaaaaaat cctggagcga acttctgtat gagggtctgc attcttcaca
      841 gccgatggag ttgacctcga aattcagttc tcttaagccg aggttttcca gaaggacctt
      901 gagcatggca atgacctcgc cgtccagttc agcagtcgcc acaccgaagg cctcaacccc
      961 aatctggtag aactgccgga atctgccctt ttgtggcctc tcgtaccgga acatcgggcc
     1021 tgaatagaag aatttctggg gaggaggcaa tgtatggaga tggtgctcca cataggccct
     1081 caccactggt gcggtgccct caggcctgag ggttatgctt ctgcctgcct tgtccggaaa
     1141 ggtgtacatc tccttttcca caatatcggt cgtctcgccg atacttcttg tgaaaagggt
     1201 agtggattct attatagggg ctttgatctc ttgaaatccg tagttcgcga atatcttcct
     1261 tgcgacagat tccactcttt gccataggta gatatccgga gggaggatgt cctgaacacc
     1321 cttgagggca ttataccgct gcgaagaact cacgatgaaa tgtcacctgc ctcttcacct
     1381 tccctttcct tgtcgaactc gagcatccgc atgtggctct cgatgagccg cttcagctct
     1441 tccttgaaat gcctgcggat gcccttaagg tccacaatgt cctcatggat cttcacgacc
     1501 ttgtcctggg cctctttcat caccccatct gccttgatct cagcttccct gatgaggagt
     1561 tccgcctctt ttctcgcatt tgtcttgtat tcctccacca tctgctgggc agtcataagg
     1621 gtctccctca aggtcgtctc catatccctg tactccttga tctggttttc cgccctgtga
     1681 atctgctctt ttaaggacgc attttccctc aggaggtcct ccatctcctc cctcacgatc
     1741 tcgaggaacg cgtatacctc ctccacatca aaccctctga acttcatggg gaactgtttc
     1801 tgctgaatgt caagcggagt gattctcata taaaggcacc tccttttatt ttatacccga
     1861 gttctaccag ggaatgtatc aaaaatcttt gaataaacat gatcgcgaga atgacgacaa
     1921 gaggtgaaat atcgataggg ccgagcctga agcctatgag cctccttatg ggcctgaaaa
     1981 ccggttcagt aacagaatgg agaaatctga cgatggggtt gtacggatcg ggattgaccc
     2041 agcttataag tgcagcaatg atcacaaccc acatgtagag cgaaagcact atatcgagga
     2101 tattcgcgat tgccagaatg agattgccaa ggacaaacat tattccctcc tccccaattc
     2161 ctctgccctc ctgagggccg cctccagggc atctctcacc aggctgccca atttcttgtc
     2221 ctggaaaacc ttcagcccag cggcggttgt tcccccgggt gatgtcacca tctccctcag
     2281 tctctcggga tgcattccgg tctcgaggag tttcgcagtg ccgatgagcg tctgtatggc
     2341 cagttcagac gcattgtccc tcgtgagtcc catggccact cctgcgccaa tcatggcttc
     2401 tgcaaagagt gcgaaaaacg caggtccgct ccctgacaac gcagtcaccg cattcatata
     2461 cgcttccgga agggtaagaa cccttcccgc tgacatgaat atctccctca ccttggcgat
     2521 ttccatatca gaagaacatt cacataatga gagcacagac attccctcct gcacaagggc
     2581 aggggtattg ggcatcaccc tgacaagccg tttggttgcc agtcttgact ggagaaaggc
     2641 aagggtgatt cctgcagcaa tggataccac tgtcttctct gcggtgatca cgtccctcac
     2701 ctcgtccaga actgctgaca tattttgggg ctttatggca aggataatga gggaacacga
     2761 agacgccacc tccctgttgt gtggggttgt ggtgatcccg taggactgct caagatattt
     2821 cctccgctcc tcgctgggct ctgacaccac tatggagctc atcccgcggg aggtcatacc
     2881 ctttatcagc gcctctgcca tattcccccc tccgatgaaa ccgatcataa ggcctcctca
     2941 agcgatttgt catattctat atcaaaacat cctctttttc tataaaggta caaagtgctc
     3001 ttatttcctc tctccaaaga tggcggtccc tatcctgacc atggttgctc cctcttcaat
     3061 ggctacctcg aaatcattgg acatacccat ggataattcg ggcagagggt gccctgccga
     3121 accagcctca tcccgcaatt ccctcagctt ccgaaagtat ggtctcgcct cttcagggtg
     3181 ctcaaaaaaa ggagggattg tcatgaggcc catgattctc agatgtttca aggcttgagc
     3241 catctttaaa aactggagga gttctgctct ggctactcca tgctttgtct cctcaggcga
     3301 gagcttcacc tccagcagca cttcctggac cctaccgatc ttttctgcct gcgagttcag
     3361 ctcttctgca agctccgca
//
LOCUS       DATKOU010000030        10308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1191031, whole genome shotgun sequence.
ACCESSION   DATKOU010000030 DATKOU010000000
VERSION     DATKOU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191031"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VMU21_01165"
     CDS             complement(<1..161)
                     /locus_tag="VMU21_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HUO76161.1"
                     /translation="MKKIEAIIKPFKLDEVKDALNEIGIQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVI"
     gene            complement(148..879)
                     /locus_tag="VMU21_01170"
     CDS             complement(148..879)
                     /locus_tag="VMU21_01170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HUO76162.1"
                     /translation="MSVLILKNIASEGPGTVEDFLRERGTYYKIVNCAIENIPGSKDF
                     DTLVMLGGPMSVNEEKLYPYIRKEMALVKEFVAKGKKIFGICLGAQIMAKALGAKVYP
                     GPEKEIGWYDIELTDRGTRDRCMKELAVHLKAKAGDFRTKFKVFHWHGETFDIPEGAV
                     RLAKSKLYPNQAFRYGERAYAFQFHIEVRKEMIYEWLKEESVDMNQIRKQTEAIYDEY
                     LKRAMNFYREFFAQKQSQEVRHEKN"
     gene            1262..1753
                     /locus_tag="VMU21_01175"
     CDS             1262..1753
                     /locus_tag="VMU21_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HUO76163.1"
                     /translation="MISTPGIRYGAIEGIVASKATFYQQAAEKTVNPIGRRVFLSVMD
                     DEKRLLEDFRGIPKERHLGTRGTASTRVIKGFLKKIKATTDDVEALKIAMDMEKESIG
                     LYEKLSKKVRTPKEKALLKSLLNEEQQRYAEFSDTCLFLSDSRSWFMWDEHSIMDGGT
                     PWA"
     gene            complement(1769..2167)
                     /locus_tag="VMU21_01180"
     CDS             complement(1769..2167)
                     /locus_tag="VMU21_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76164.1"
                     /translation="MRIFWSFAGIMVACMCQWLFLSSAALAADECEVVKASYYERTQG
                     EIVTDWSGGYAYRRYQTVVYPCADVTVRDTYGSAFIRVVEITATFSDQGKASKKAWCD
                     KKTLENDVTYFCIVCFENEFPVTNVACNFR"
     gene            complement(2283..2609)
                     /locus_tag="VMU21_01185"
     CDS             complement(2283..2609)
                     /locus_tag="VMU21_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76165.1"
                     /translation="MKRTSKLNCWEFKRCGRQLGGLRINDLGRCPATLEERLDGVHDG
                     VYAGRACWVVAGTFCRNEVQGTFAQKFESCQKCDFYEKVREEEYPNFELSASLLSKIK
                     KTTKHV"
     gene            3001..3231
                     /locus_tag="VMU21_01190"
     CDS             3001..3231
                     /locus_tag="VMU21_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HUO76166.1"
                     /translation="MITTKEMVLQAEDITCSGCAGDMEAILRKREGVMEASVDFATGI
                     IRVRYDQLLLDRKAVFSAVRSLGYKVRILEEK"
     gene            complement(3313..3765)
                     /locus_tag="VMU21_01195"
     CDS             complement(3313..3765)
                     /locus_tag="VMU21_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HUO76167.1"
                     /translation="MAALCDDCTRLTYAEILKDKKASTLTYFMARSLSWFKQIYHFEF
                     DAVMSDNGSEFKGTLEREHPFETMCIELGIRHLCTRPYRPQTNGKIEAFWKIIKKEFF
                     TPNSFDSHDDMVLNLGNFLFEYNHLRKHGGLNYETFFDKLQKVTELLR"
     gene            complement(3732..4271)
                     /locus_tag="VMU21_01200"
     CDS             complement(3732..4271)
                     /locus_tag="VMU21_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76168.1"
                     /translation="MLYSEQVIVKRWIPILKEYEKTKAKETPRVFKFIKDLCEAHHIS
                     RKELGRYHRKWVQGNRTEEALLPRRRGARPGSRRTPREMERAIIKAYRRFGSNRYELV
                     LPFKPYYLDKTRLRQRWTGSRHAIHLMKPRRRSSNDMKSRPRESWPISTYHSSPRISA
                     APLSSRSVIWQRCVTIARG"
     gene            4538..4819
                     /locus_tag="VMU21_01205"
     CDS             4538..4819
                     /locus_tag="VMU21_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76169.1"
                     /translation="MPKQNCWEFKQCGREKENEEGNGTCPAVTEAAADGVNSGTNGGR
                     ICWAVAGTYCDGSVQGTYAQKVTSCLMCEFYMRVKGEEGYQFKRIHFKF"
     gene            5063..5323
                     /locus_tag="VMU21_01210"
     CDS             5063..5323
                     /locus_tag="VMU21_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76170.1"
                     /translation="MTHLARRLEELFTEITFAEDRVFSPVQKTLRKVGQKIDDTFIAI
                     TFAEAGVFDRALSTLNRDGKTPTHRKSGYRPKRFTKLCSGRV"
     gene            5367..6371
                     /locus_tag="VMU21_01215"
     CDS             5367..6371
                     /locus_tag="VMU21_01215"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HUO76171.1"
                     /translation="MKPVPPKGTAKGQGWILFFYSVPSKPVSNRMKVWRRLTKAGAVQ
                     LKGSVYILPYNDEHYEFLQWLVSEIAEMKGEAGFVKIEKIDTMKDPEIITLFDTQRTD
                     DYKNIGKALDDLERRLNSTQKGGKAQNIEDISEQFAKVLRGYEEVQRVDFFSSREGQA
                     LNQRIRRTKTNLKTLSGVEQGKEEAVAITPKRVEDYHGRTWVTREKPFIDRMASAWLI
                     RRFIDKKASFEFIDERDMDSIGRGPIVFDIRGGEFTHIGDMCTFEVLVKCFGLKDKVL
                     KKMAEIVHDLDLKDEKYKSAEAKGLEDILIGIRKAAKDDSDALEKGMTVFEMLYVSKS
                     "
     gene            6523..7692
                     /locus_tag="VMU21_01220"
     CDS             6523..7692
                     /locus_tag="VMU21_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HUO76172.1"
                     /translation="MKTISSSLLTSACSFRGLGPSSWQYTSAGCSIGRGEAFVAGVFF
                     VLPSVFILWGLAWLYTSFGNVPSVGAFFYGLKPAVAAIVAEAVIRIGKKSLRNWVLIM
                     LAAFSFIGIYFLNIPFPVIVLSAGAIGFLGSFIAPSFFFKGKSEEAHHADVVLNDEML
                     AQDRVNPTWLRSLKVMAVCLTFWFLPLIMIGLWRGWNDVLVDIGVLFSKATVVTFGGA
                     YAVLAYISQQAVGHYNWLTPEQMIDGLGLAETTPGPLIMVGQFIGYLAAYTNAQGIQP
                     AVAGAIGGFLTTWVTFTPCFLWIFLGAPYIEKLRNNVKLGSALSTITAAIVGVILNLS
                     VTFTYHVLLPEGKGFDWYALFASVVAFVGMIRFKWGMIPVIIGSALAGYIWKMLI"
     gene            7745..8299
                     /locus_tag="VMU21_01225"
     CDS             7745..8299
                     /locus_tag="VMU21_01225"
                     /inference="COORDINATES: protein motif:HMM:NF021439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HUO76173.1"
                     /translation="MKEVTEMAVKLMAASARTAPKAGWKDFLEIVVITKDEDLKKIAD
                     AMKQYAPKSTNEAYWLRDASNIENSQALLLVGLSKPVTAGYDCGGCGYPTCKEFEKVK
                     QMKDKEMGYTGPHCVMRMIDIGVALSSAAKTASLLNVDNRVQQRVGAAARALGLIKGE
                     VVMGIPVSVTGKSIYYDRETPVKH"
     gene            8467..9675
                     /locus_tag="VMU21_01230"
     CDS             8467..9675
                     /locus_tag="VMU21_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUO76174.1"
                     /translation="MTSILSPFTALCSVGFFARLSYALARSPVLPLFALYLGAGPEAI
                     GFAVGISTVTGIFFKLPAGALSDVIGRKRTMMVGLLFFAFMPFAYLLVKDYNLLIIIR
                     FLHGLATAIYGPVSMAVVADIAGAKKGEMLSWFSSVTIIGNLLGAPLGGFILHRSPGA
                     ANPSLIDFQCAYLASGIAGLLSLILAIGLLRGDKSTSDGRGLKEASLKFISGIREVIS
                     NKRVAITSAIEGLQNMTMGALEAFLPIYAVKVVGLNEFQAGILWGVQVVTTVLSKPIM
                     GRTSDKYGRKALISVGILICAVSFGLIPVFKNFYLLMLAAAFFGLGEALVTSSGAAFV
                     ADLCKRKHFGTAMGTFGTIFDIGHASGPILAGFLLAPHGYLFSFWVMSAALVIAVPLF
                     IMTVHVERNA"
     gene            9761..>10308
                     /locus_tag="VMU21_01235"
     CDS             9761..>10308
                     /locus_tag="VMU21_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76175.1"
                     /translation="MKTLGTLLMGVTMFGIMFSSVVALESKKFDFNNETLGAESKAFS
                     ALVGDWRIDKDGSNLVYAVDGRERVPKLSAGADGHARDLYGKKFAEFLKGIEPLKDYP
                     LTASKEFQHFTNGTLTVSFKAISGIGDQAAGIAFSIRQNGEYLAMRANALENNLGLFK
                     FQKGKRSPLQWAQDVPPPERGW"
BASE COUNT         2745 a         2402 c         2469 g         2692 t
ORIGIN      
        1 tctatgacat attcagcccc gcgatagagc tctgtgtgtc ccttctgcct cccgaaaccc
       61 ttcacctctg tcacggtcat tccctggatg ccgatttcat ttaaggcatc tttcacttca
      121 tcaagcttaa agggcttgat aattgcttca atttttttca tgtctcacct cctgcgattg
      181 tttttgtgcg aagaattctc tataaaaatt catggccctt ttgaggtact cgtcatagat
      241 cgcttctgtc tgcttcctta tctggttcat gtcaacagat tcttccttca accattcata
      301 gatcatctct ttcctgacct ctatatgaaa ctggaatgca tatgcccttt caccatatct
      361 gaatgcctga tttgggtaga gtttcgactt cgcaagcctc accgcaccct caggaatatc
      421 aaatgtctcg ccatgccagt ggaatacctt aaactttgtc cggaaatccc ctgcctttgc
      481 ctttaaatga accgcgagct ccttcataca acggtccctt gtgcccctat ccgtcagctc
      541 gatgtcgtac caacctatct ctttttcggg tcctggatag acctttgcac ccaacgcctt
      601 tgccatgatc tgcgcaccga ggcatatccc aaaaatcttt ttccccttcg ccacgaattc
      661 ctttacgagc gccatctctt tccttatata gggatacagt ttttcctcat tcacgctcat
      721 cggtcctcca agcattacaa gcgtatcgaa gtcctttgat ccggggatat tctcaatagc
      781 gcaattcacg atcttgtaat acgtccccct ttcacggagg aaatcttcaa ccgtacccgg
      841 accttctgat gctatgttct ttaaaatcag gaccgacatt ctatgctata aatacaataa
      901 gcgtgccatt aacaggcatc tggaaaataa gggttttttc ttataacgcc aagataattg
      961 cctacaaata aggcagaggt ggctgagaaa agggcacttt cgtgggaaac atcctgtact
     1021 tataaaagca cataaatgac cttcggatat atccttccat tgccgggcct caagatgaac
     1081 cattccgcct cctgttaagc gcaataaatt gcgcactaaa ttttgcatgt tttgcaatat
     1141 atttcatagt tagagagatc cccaagacac acaatcattt aaaatcaaac aggttagtta
     1201 atggaatagt atttgcttca ttatataata ggttccaaaa tttatactga caggaggcac
     1261 tatgatatca acaccaggaa ttcggtatgg agcgattgaa ggcatagtag catccaaggc
     1321 aacattttat cagcaagccg cggagaagac ggtgaacccc attggaagaa gggtattcct
     1381 ctctgtaatg gacgacgaaa aaaggcttct tgaagatttc aggggcatcc cgaaggagcg
     1441 ccacttggga acgcgcggca cagcatccac aagagtgata aaaggctttc ttaagaagat
     1501 caaagcaacc acggatgatg tggaggcctt gaaaattgcc atggacatgg aaaaagagag
     1561 catcggactc tatgagaaac tttcgaaaaa ggtacggacc ccaaaggaaa aggccctttt
     1621 aaaaagtctc cttaacgaag agcagcagcg gtatgcggaa ttctctgaca cctgtctttt
     1681 cctttctgac tcacgcagct ggttcatgtg ggacgagcac agcatcatgg acggcggtac
     1741 cccatgggca taacaagaaa agagaccctc acctgaaatt acaggccacg ttggttacgg
     1801 gaaactcatt ttcaaaacag acgatacaaa aataggtgac gtcattctca agggtctttt
     1861 tatcacacca cgcctttttt gaagctttgc cctggtcgct gaaggtcgcg gtgatctcaa
     1921 caacccttat aaacgcgctc ccgtacgtat cacgtaccgt cacatctgca cagggatata
     1981 ccactgtttg gtatctcctg tacgcataac cgcccgacca gtcagtcaca atctccccct
     2041 gcgtcctttc ataataagag gcctttacca cctcgcattc atccgcagca agagccgcgc
     2101 tactcaaaaa gagccattgg cacatacacg ccaccataat ccccgcaaaa ctccagaaaa
     2161 tgcgcatccc gccaatgcct cctttttcaa tgcctcatca gtctctatca caatagcatt
     2221 ttttctatga ccatggaagc cgtctctgtg ctcttcgcag atgcctcaca tccttgttca
     2281 tcctatacgt gctttgttgt tttcttaatc ttagaaagca gggaagctga aagctcaaaa
     2341 ttggggtatt cctcttcgcg tactttttca taaaagtcac atttctggca actctcaaat
     2401 ttctgagcaa atgtgccctg cacttcattc ctgcagaaag ttcccgccac aacccagcag
     2461 gccctgccgg cgtaaacccc gtcatgcaca ccatcaagtc tttcttctaa ggtcgcagga
     2521 caccttccca gatcattaat acgaagtcct cccagctgcc gcccacatct tttgaattcc
     2581 caacaattca atttggatgt tcttttcatt gcgagatttc tccctttcct tgaaaaagat
     2641 taacataaga tgcatagcgt tcataagacc ttcttcataa actttactgc aagtgttatg
     2701 cctacagatg tttcttgata tcttgatgcc ccacgagcac aaatattact attttataat
     2761 taggtatcgc attcatcctg aatagcccta taaatcaaca aaaaggtaat taattgagaa
     2821 aatgtccgaa aataaaacaa ggtggccgat tttcctccaa ctgacgactc ataaagattt
     2881 atcagcgtac caatataaaa agttttatct gctgttcgtc atcagagcca tcaccgtaaa
     2941 gatgaacggg ggtgaggaag ggctccctcc tgccgctctt atttgctaag atacattgca
     3001 atgataacta caaaagagat ggtcctccag gctgaagaca ttacctgttc gggttgcgct
     3061 ggtgatatgg aggcaatcct gcggaagagg gaaggtgtca tggaagcctc ggtcgatttt
     3121 gcgaccggga taatccgcgt gagatacgac cagctgctcc tggacagaaa agctgtgttc
     3181 agcgcagtac ggagcctggg gtataaggtt aggattttgg aagagaaata gggaaatgat
     3241 ctgcctgcgg agcctgcagg ttttttgtga tctcgtctga attcatctta ttctaaaagg
     3301 cagcctgttg tactagcgca ataattcagt aactttttga agtttatcaa aaaacgtctc
     3361 ataattgagc cctccgtgct ttctcagatg attatactcg aacaggaagt ttcctagatt
     3421 aagaaccata tcgtcatgtg agtcaaaaga gttgggggta aagaactcct ttttaatgat
     3481 cttccaaaac gcttcgatct tgccgttggt ctgcggccta tagggcctcg tgcagagatg
     3541 tcgtattccc agctcaatgc acatagtctc aaaagggtgt tcacgttcca gggtcccttt
     3601 gaattctgat ccgttgtcag acatcacggc atcaaactca aaatggtata tctgcttgaa
     3661 ccatgacagt gaccgcgcca taaagtaggt cagcgtcgat gccttcttgt ccttgagtat
     3721 ctcggcatag gttagcctcg tgcaatcgtc acacagcgct gccatataac actccttgag
     3781 cttaagggag cagcggatat ccttggggag ctatgatacg tcgatatggg ccaactctcc
     3841 cggggcctgc ttttcatatc gtttgatgac cttcttctgg gcttcattaa gtggatagcg
     3901 tgccttgatc ctgtccatcg ttgccgcaga cgggtcttgt ccaaatagta cggcttgaac
     3961 gggaggacca actcataacg gttcgatcca aatcttcggt atgccttaat tatggcccgc
     4021 tccatctccc ttggcgttcg ccgtgaacca gggcgcgctc cacgcctgcg gggcagaagg
     4081 gcctcctctg tcctgttgcc ctgtacccac ttgcggtgat atcgaccaag ctcttttctc
     4141 gatatatgat gcgcctcgca cagatccttg atgaacttga agacacgagg cgtttctttt
     4201 gccttggtct tctcgtattc cttgagtatc ggtatccatc tctttactat cacctgctcc
     4261 gaatatagca ttaggccccc ctttggcgat atcgtaccaa aagagttacc taattattga
     4321 gcatgtacac attagaatgc gcacggtatc ggttaatcaa gacaacgcaa cagcagacga
     4381 aataaaactc ctcatcaaag aatgatttgc agcctttatg cgtgaataag aacaaattat
     4441 ctatagacag ggcttcgcaa ggcaacgtat aatacaaggt ctgcattatc aggaacacga
     4501 tcaaggatag ggcatttgcg gtcagaaggg agaatgcatg cctaaacaga attgctggga
     4561 attcaaacag tgcggcaggg aaaaagagaa tgaagaggga aatgggacct gccctgcggt
     4621 aaccgaagcc gccgctgacg gagtgaacag cggaacaaac ggggggagga tctgttgggc
     4681 cgtagccggg acctattgtg acggcagcgt gcaggggaca tacgcacaga aggtcacctc
     4741 atgtctcatg tgtgaattct acatgagggt aaaaggggaa gagggttatc agttcaaacg
     4801 catacatttt aaattctgac atcagactgc ctgcagtata acaatccgta aagtccttat
     4861 gtttcaggcc cgatccaaag caactttcga ataatagtgt gattctgcat tctaaggaac
     4921 gcccgaaaag tactgttgcc agaacctaga atcgttatat cgtgaccggg ggagcttttt
     4981 tcacttgaca agtaatgtaa catctgttac attattatca gaagttactt tttaaaggca
     5041 acaaccctaa aaggagggcg ccatgacaca cttagcgagg agactggaag agttgttcac
     5101 agagataacc tttgcggaag atcgggtatt cagcccggta cagaaaaccc tgcgcaaggt
     5161 tggacagaag atagacgata cctttattgc aattaccttc gcagaggctg gggtgtttga
     5221 cagagcactg agcactctca acagggatgg gaaaacaccc acgcatcgca agtccggata
     5281 tcgtccgaaa agatttacaa agctttgctc cggcagggtt taacaaagct gatattcgag
     5341 gagtcaagga ggagcagaaa aaaaccatga aaccggttcc ccccaaaggc actgcaaaag
     5401 gacagggatg gatactcttc ttctatagcg tcccttcaaa gcctgtgagc aacaggatga
     5461 aggtctggcg aaggctcaca aaggcagggg ctgtgcagct gaaaggctcg gtctacattc
     5521 tgccctataa tgatgagcac tacgaatttc ttcaatggct ggtatctgaa attgcggaga
     5581 tgaagggtga ggcaggattt gtaaagattg agaagatcga cacgatgaag gaccccgaga
     5641 taatcaccct gtttgatacg cagaggacgg atgactataa gaacataggc aaggccctgg
     5701 atgaccttga gagaagattg aacagcacac aaaaaggtgg aaaggctcag aatattgagg
     5761 atatatcaga gcagttcgca aaggtcctca ggggctatga agaagtgcaa agagtggact
     5821 tcttctcatc aagagagggt caggcattga accagagaat aaggcgcaca aaaacaaatc
     5881 tcaaaaccct ttcgggtgtt gaacaaggca aagaagaggc tgttgcaatc acacctaaga
     5941 gagttgagga ctatcatggg aggacatggg taacaaggga aaaaccattt atagacagaa
     6001 tggcttcagc ctggctcatc agacgtttta tagacaagaa agcttctttt gagttcatag
     6061 atgagaggga tatggactca ataggcaggg gccccattgt ctttgatatc cgcggaggag
     6121 agttcaccca tataggtgat atgtgcacct ttgaggtgct cgtcaaatgc tttggtctga
     6181 aggacaaagt tctcaagaag atggcagaga tcgtccatga ccttgacttg aaagatgaaa
     6241 aatacaaatc tgccgaggca aaaggcctag aggatattct catcgggata cgaaaggcag
     6301 caaaagatga ttcggatgcc ctcgaaaaag ggatgacagt gtttgagatg ctgtatgttt
     6361 caaaaagtta atttataaag ggagaactca tcttgggaac taaaccgtca tttagggaag
     6421 cctttcacta ctggttcaag cttggcttca tcagttttgg ggggccaaca ggacagattg
     6481 caatgatgca taaggacctg gttgaaaaaa agagatggat tgatgaagac catttcctcc
     6541 agtctcttaa cttctgcatg ctccttccgg ggcctggggc ccagcagctg gcaatataca
     6601 tcggctggct gctccatcgg acgtggggag gcatttgtcg cgggggtctt cttcgtgctc
     6661 ccctcggtat tcatactatg ggggcttgca tggctgtata cctcttttgg taatgtccct
     6721 tctgtggggg ccttcttcta cgggctcaag ccggcagtgg ctgcgatagt ggccgaagct
     6781 gtcataagga ttggcaaaaa gtccctcagg aactgggttt tgataatgct ggccgcattt
     6841 tcatttatag ggatctattt cttgaacata ccgtttcctg ttatcgttct ttcagcaggc
     6901 gccattggtt ttcttggcag ttttattgcc ccgtcctttt tctttaaggg gaagtcagaa
     6961 gaagcccatc atgccgatgt tgtgctgaat gacgaaatgc tggctcagga ccgcgtcaat
     7021 cctacctggt tgcgctccct aaaggtgatg gctgtctgcc tcaccttctg gtttcttccc
     7081 ctgattatga tcggtttatg gaggggctgg aacgatgtcc ttgtggacat cggcgtcctc
     7141 ttcagcaagg ctactgtggt aacgttcggt ggggcttatg ctgtgcttgc gtacataagc
     7201 caacaggcag tggggcacta taactggttg acgcctgagc agatgattga cggccttgga
     7261 ctggcagaaa caacccccgg cccgctcatc atggtaggtc aatttatcgg ataccttgcc
     7321 gcatacacca acgcacaagg gatccagcct gcggttgcag gggccatcgg cggttttcta
     7381 actacatggg tcaccttcac cccctgcttc ctatggatat tccttggagc cccttatatt
     7441 gagaagctcc ggaataacgt caaactggga tcagcgctct cgacaattac cgcggcgatt
     7501 gtcggtgtaa tcctaaacct ttccgtgaca tttacttatc acgtccttct gcctgagggt
     7561 aaaggatttg actggtacgc gttgtttgcc tccgtggttg cctttgttgg aatgatccgc
     7621 tttaaatggg gaatgatccc ggtgataatt ggctctgccc tggcagggta tatatggaag
     7681 atgcttatct aatgaagggc aagtagttgc ccttacttga gtcgtatgtc aaaggaggtt
     7741 cgcaatgaaa gaagtaacag agatggcggt aaagttgatg gcagcgtctg cgaggactgc
     7801 tcccaaggct ggttggaaag actttctcga aatcgtcgta atcacaaagg atgaagatct
     7861 taagaagatt gctgatgcga tgaagcagta tgcgccgaag agcacaaatg aggcatattg
     7921 gctgagggat gcgtccaaca tagagaattc ccaggccctt cttcttgtag ggttaagcaa
     7981 gccagtaacc gcagggtatg attgcggagg atgcggttat cccacctgta aggaatttga
     8041 aaaggttaaa cagatgaagg acaaggagat gggatacaca ggcccccatt gtgtgatgag
     8101 aatgatagat atcggcgttg cactgtcatc agcggcaaag acagcaagcc ttctgaatgt
     8161 cgacaacagg gtccagcagc gtgttggcgc tgcggcaagg gctctgggac ttatcaaggg
     8221 cgaggttgtg atgggcatac cggtgagcgt cacaggaaag agcatctatt acgaccgcga
     8281 gacaccggtg aaacactaag aaaaacataa tgcatagaca ggcaggacac ggtatttgag
     8341 cggtttcttt tgctgatagt ccaaatacaa tatctaaata tcgagaggca aaagcctcgg
     8401 aggtcggaac gaccgagaat gagcaaaaat atcgtgtcct gcctgcgtat gaggtagatt
     8461 agatttatga caagcattct ctctcccttt accgcactct gctcggtcgg cttctttgcc
     8521 aggctgtctt acgcccttgc aagaagcccg gtgcttccgc tttttgccct ctatctcgga
     8581 gcaggtcccg aggcgatcgg gtttgctgtg ggaatatcga ccgtaacagg gatatttttc
     8641 aaacttcccg caggtgcgtt atcagatgtg ataggcagaa agaggacaat gatggtcggt
     8701 cttctctttt tcgccttcat gccctttgcc tatcttctgg tgaaggatta caacctactt
     8761 attattatca ggttcttgca cggtcttgca acggcgatct acgggcctgt gtccatggca
     8821 gtagtcgcag atatcgccgg cgcgaaaaaa ggagagatgc tttcgtggtt ttcttcagta
     8881 accataatcg gcaatctcct gggtgctccg ctaggcggct ttatactcca cagaagcccc
     8941 ggagccgcaa acccctccct cattgatttt cagtgtgcat accttgcaag cggtattgcg
     9001 ggtctcctat cgcttatttt agcgatcggt ctactgcggg gagacaaatc tacatctgat
     9061 gggagaggac tcaaggaagc atccctgaag ttcatctcag gaataagaga ggtcataagc
     9121 aataaacggg ttgcaatcac ttctgcaata gaaggtcttc agaacatgac catgggtgcc
     9181 ctggaggcct ttctgccgat ctacgcggta aaggttgtcg gtctcaatga gtttcaggca
     9241 ggtatcctct ggggagtgca ggtggtaacc acagtactct caaagccgat tatgggaagg
     9301 acatcagaca aatatgggag aaaagcattg ataagcgttg ggatcctcat atgcgcggtc
     9361 tccttcggcc tgataccggt attcaaaaac ttttatctgc ttatgcttgc cgctgcgttc
     9421 ttcggcctgg gagaagcact cgttacctct tctggcgcag cttttgtagc agatctctgc
     9481 aaacgaaaac acttcgggac tgccatgggg actttcggta cgattttcga tatagggcac
     9541 gcatcaggcc ctatacttgc cgggttcctc ctggcacccc acggttatct tttttcgttc
     9601 tgggtaatgt ctgctgccct tgtgatcgca gtgcctttgt ttatcatgac tgtgcatgtt
     9661 gaaagaaatg catagaggcg tcattattac tctggtcact attctttctc ctttgtacgc
     9721 ggcctctatt gggatcaata gaattcggca ggaggaacat atgaaaacgc tcggaacact
     9781 tttaatgggg gtaaccatgt ttgggataat gttttcttcc gtagttgcac ttgagagtaa
     9841 gaagtttgac tttaataatg aaacactcgg ggctgaatca aaggcattta gcgcgctggt
     9901 tggcgactgg cgcatcgata aggatggctc gaaccttgta tatgccgttg atggcaggga
     9961 gagagttccg aagttatcag caggcgctga tggtcatgca agagatctgt acggcaagaa
    10021 gttcgccgaa tttctcaaag gcatcgaacc attgaaagat taccctctca ctgcaagcaa
    10081 ggaatttcaa cacttcacaa atggaacgct gacagtatcc tttaaagcaa tcagtggaat
    10141 aggagaccag gcagccggaa ttgcattcag tatcagacag aacggtgagt atcttgctat
    10201 gagggccaat gccctggaaa ataatctcgg gcttttcaag ttccaaaagg ggaaacgttc
    10261 acccttgcaa tgggcacaag atgtccctcc gcccgaaaga ggatggca
//
LOCUS       DATKOU010000031         5778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1191283, whole genome shotgun sequence.
ACCESSION   DATKOU010000031 DATKOU010000000
VERSION     DATKOU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191283"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VMU21_01240"
     CDS             <1..832
                     /locus_tag="VMU21_01240"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545315.1"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HUO76176.1"
                     /translation="LDMAIGQASLWAASNVEPDKVVSEIMPTEPYLLGSHAGCAGFWV
                     SGPGDIPGTPAHYTWGYNRMSTVPGLFMAGDVVGASGHKFSSGSHAEGRIAAKAAIAF
                     ILDHPDYKPTPKKSPDEVAAEIYLPFEIYEKYKTYTTAPDINPNYISGKALQARLMKI
                     ADEYFGGVSTWYMTSKTMLEEGLKKLELLKEDAGKMAAKDLHELLRCNENYHRILSVE
                     AHARHILFREESRYPGYYYRGDHNKIDDVNWKCFTNSKYDAATNKWEFKKVPYVTMFP
                     "
     gene            960..2219
                     /locus_tag="VMU21_01245"
     CDS             960..2219
                     /locus_tag="VMU21_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HUO76177.1"
                     /translation="MAENKTVLVIGGGFSGLTAAVEAAEAGCDVVVVEKNPYLGGRVA
                     QLNKYFWKLCPPVCGLEINFKRIKNNGQITFHTLAEVEEIKGEEGNFEVKVKLSPRYV
                     NDKCVGCDACAQACPSYRSNEFNFGMDKTKAAYLPFNQAFPFQYVIDDAACTKDCMKA
                     CADACKYDAIDLNMKPETKTLKVGAVIMATGWNLYDATRMDNLGFGKVRNVITNMMME
                     RLAAPNGPTEGKILRPSDGKEVKNIAFVQCAGSRDENHLPYCSYICCLASLKQTMYVR
                     EQYPDSKAQIFYIDLRTPGLYENRFLWKVKDDPNVTLTKGKVAGVTEDPQTKDVIVEV
                     EDIYGGKKIKASFDMVVLAAGMVPGTKDTKIMKDIPYTPDGFVDASGTKKGIYAVGTL
                     KSPVDVARSVQDATGIVMKSIQSLRRK"
     gene            2222..4453
                     /locus_tag="VMU21_01250"
     CDS             2222..4453
                     /locus_tag="VMU21_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HUO76178.1"
                     /translation="MEKKYGVYICKGCGIGEAVDIEKLTNIATKDMKVPLCKTHDILC
                     SPEGLQLIKDDIKNEGVNTITIAACSPRVKYEEFDFPGTITERANIREFVAWTQPAMT
                     DETQSVAEDYLMMGIVKTQKGDLPEPNILEGLSNTLLVVGGGISGMTAALSAANAGQQ
                     VSLVEKDPQLGGFVNKLYKKIPTGVFFREPLIVDTEIESLIKEVESNPNIKVYKSARV
                     EKTEGQPGLYEVTIASNGNSETVKIGAIVMATGWKPYDATKLDYLGYGKFRDVVTNIE
                     FEEIAKKGKIARPSDGKEAKKVAFIQCAGQRNPNHLPYCSSMCCATSLKQAKYVRQNP
                     DAAAMIFYIDMRTPGRLETYYKEAQNDPGVMLTKADVTAVSDAGNGNLFVEMDNSLLG
                     EKVKVEADLVVLATGMVPTTRGPQEYLDKFTQEAAKGDDAKKAFLETVPKPEFILNLT
                     YRQGPEIPSLEGACGFADSNFICFQYETRRTGIYAAGSVKQPMNMSEAQEDAAGAAFK
                     AIQCMDHIAKGMAVHPRAWDLTFPDPMMIKCTSCKRCTEECPFGAIDEDEKGTPFYRL
                     NRCRRCGTCMGACPERIVHFKDFSVDIIGSMIKKIDSPDEETFRILVLVCENDAYPAL
                     DTAAIKRKAIDPSVRFISLRCLGGMNLVWIADALSKGIDGVLLLGCKYGENYQCHFVK
                     GSELANYRFSKVSETLNRLQLEAERVQMMQVAINEYDKLPDMINEFVEKVKEIGPNPF
                     KGM"
     gene            4537..5658
                     /gene="qmoC"
                     /locus_tag="VMU21_01255"
     CDS             4537..5658
                     /gene="qmoC"
                     /locus_tag="VMU21_01255"
                     /inference="COORDINATES: protein motif:HMM:NF038018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-interacting membrane-bound
                     oxidoreductase complex subunit QmoC"
                     /protein_id="HUO76179.1"
                     /translation="MAEQVISPDVNFVKDVIRNGGESLKKCFQCATCSVVCNVTPENK
                     PFPRKEMILAQWGQKEQLISNPDIWLCHQCSDCTAYCPRGAKPGEVLGAIRKITIKEF
                     SAPSLLANMVSDAKYLILLFALPVLILLGVTSYHGTLTEVPRVDGKIVYFKFIPIHFG
                     EFGVDLIFTAAAVFAALSFFFGVKKYWAAMSKGAQLKGDVVGSFKEVISDILSHDRFK
                     KCNVTKARSTSHLLVFYAFIGLAITTSWAVFYLYIMNRPSPYPLYDPLKLLGNVSALA
                     LLAGIVLVITNRLKNQEKAGIGSYYDWLFITVVFAIMATGILSEVFRLLDIAPLAYPT
                     YFTHLVFVFFLFAYAPFSKMAHMVYRATAMVFAIEAGRE"
BASE COUNT         1515 a         1356 c         1598 g         1309 t
ORIGIN      
        1 cctcgacatg gcaatcggac aggcgtcact ctgggcggca agtaatgttg agcccgataa
       61 ggttgtttcc gaaataatgc cgacagagcc gtatctcctg ggttcacatg caggctgcgc
      121 agggttctgg gtaagcggcc cgggcgatat tcccggcaca ccggcacatt acacctgggg
      181 atacaacagg atgtctaccg tgccaggtct cttcatggcc ggtgacgttg tgggcgcctc
      241 aggccacaaa ttctcatccg gttctcatgc agagggcagg atcgcggcca aggctgcgat
      301 tgcctttatt ctcgatcacc cggactacaa accaactccg aaaaagtctc ctgatgaagt
      361 cgcggccgag atctatctgc cctttgagat ttatgagaaa tacaagacct atacaactgc
      421 ccctgacatt aaccccaatt acatcagcgg gaaggcgctt caggcaaggc tgatgaagat
      481 cgccgacgag tacttcggcg gtgtctcaac atggtacatg acctcaaaga ccatgctcga
      541 agaaggtctc aagaagcttg agttgctcaa agaggatgcg ggcaagatgg ctgcaaaaga
      601 cctccatgag ctcctgaggt gcaatgagaa ttatcacaga attctgtcgg tcgaggcaca
      661 tgcaaggcac atcctcttca gggaggaatc caggtaccct ggctactact acaggggcga
      721 tcataacaag atcgatgatg tgaactggaa gtgcttcacg aactcaaaat atgacgctgc
      781 aaccaacaaa tgggaattca agaaggttcc ctatgtcacg atgtttcctt aactgattgg
      841 attaggggag ggcaaatggc cctcccctaa tttttctgta ggtcaaaaag aggcatttat
      901 attatttagc atgagatata agtatctctt tgtgacttac tgtttcaagg aggatgaaga
      961 tggcagaaaa taaaacagtt ctcgtgatcg gcggcggttt cagcggactt accgcggcag
     1021 ttgaggctgc tgaggccggg tgcgacgtag tcgtcgtcga aaagaaccct tatctcggcg
     1081 gcagggtggc gcagctgaac aagtatttct ggaaactctg tccccctgtc tgcggactag
     1141 aaataaattt caagaggatt aagaacaacg ggcagatcac gttccacacc cttgcggagg
     1201 tggaagagat caagggcgag gaagggaact ttgaggtgaa ggtgaaactc agtccccggt
     1261 acgtgaatga caagtgcgtg ggctgtgatg cctgcgcgca ggcatgtcct tcctaccgga
     1321 gcaatgaatt caatttcggc atggacaaga ccaaggctgc ctatcttccc tttaatcagg
     1381 cgttcccctt tcagtatgtg attgacgatg ctgcctgcac aaaggactgc atgaaggcat
     1441 gcgccgatgc atgtaaatat gatgcaattg atctgaacat gaagcccgag accaagaccc
     1501 tgaaggtagg ggcggtcatt atggccacag ggtggaatct ctatgacgcc acgagaatgg
     1561 ataaccttgg gtttggcaag gtccggaatg tgatcacgaa tatgatgatg gagaggctcg
     1621 cagcccccaa cggaccgacc gaaggcaaaa tcctccgccc ctctgatgga aaagaggtga
     1681 agaacatcgc ctttgtccag tgcgcggggt caagggatga aaaccacctc ccctactgtt
     1741 cctacatctg ctgtctcgct tcgctcaagc agaccatgta tgtccgtgaa caatatcccg
     1801 attcaaaggc gcagatattc tacatcgacc tcaggacccc cgggctttat gaaaacaggt
     1861 tcctctggaa ggtgaaggac gatcccaatg tcaccctcac caagggcaag gtggcagggg
     1921 ttacggaaga cccgcagacg aaggatgtga tcgtggaagt ggaggacatc tatggaggaa
     1981 agaagattaa ggcctccttt gacatggtgg tgcttgcagc gggaatggtt ccagggacaa
     2041 aggatacaaa aatcatgaaa gacatcccct atacgccgga tgggtttgtg gatgcgtcag
     2101 ggacaaagaa ggggatttac gcggtaggga cgctgaagag ccctgttgat gttgcgcggt
     2161 cagttcagga tgcgaccggg atagtgatga agagcatcca gagtttgagg aggaaatagg
     2221 gatggagaag aaatacggcg tatatatctg taaaggctgc gggattggag aggcggttga
     2281 tatagagaag ctcaccaata ttgcgacaaa ggacatgaag gttccgctct gtaagaccca
     2341 cgatattctc tgcagtcctg aaggtctgca actcatcaaa gatgatataa agaatgaggg
     2401 agtcaatacc ataacgattg cagcctgctc tccccgtgtc aagtatgaag agttcgactt
     2461 ccccgggacg ataacggaac gggccaatat acgggagttt gttgcctgga cccagccggc
     2521 catgaccgat gagacccaga gcgttgcaga ggactatcta atgatgggca ttgtcaagac
     2581 ccagaagggt gaccttccgg agcccaatat ccttgagggg ctgagcaaca ccctccttgt
     2641 ggttgggggt ggcatctcag ggatgacggc tgccctctcc gcagccaacg ccggacagca
     2701 ggtgtcgctt gtggaaaaag atccccagct gggaggcttt gtgaacaaac tctacaagaa
     2761 gatacccaca ggggtatttt tcagggagcc cctcattgtg gataccgaaa ttgaatcgct
     2821 catcaaagag gtggagagca atccaaatat aaaggtctat aaatcagcac gggtggagaa
     2881 gaccgagggg cagccgggcc tctatgaggt gaccatagcc tcaaacggca attcagaaac
     2941 cgtaaagatc ggggcaatcg taatggccac tggctggaag ccctatgatg ccacgaagct
     3001 ggattacctt ggatacggaa aattcaggga tgtggtgacc aatattgaat ttgaggaaat
     3061 tgcgaagaag ggaaagatcg caaggccatc tgacgggaag gaagcaaaaa aggtggcctt
     3121 tatccaatgc gcgggccaga gaaacccgaa ccaccttccc tactgctctt ccatgtgctg
     3181 tgcaacatcc ctgaaacagg caaaatatgt gcgccagaac cctgatgctg cagccatgat
     3241 cttttacata gacatgagga cacctgggag gctcgagacg tattacaaag aggcgcagaa
     3301 cgatccgggg gtgatgctca cgaaagcgga tgtgactgcg gtgagcgatg caggcaatgg
     3361 gaacctcttt gtggagatgg ataattccct cctgggagag aaggtgaagg tagaggctga
     3421 ccttgtggtg cttgctacag gcatggtccc taccaccagg ggaccgcagg agtatctcga
     3481 taagttcacc caggaagcgg ccaagggtga cgacgcgaag aaggcatttc tcgagacggt
     3541 ccccaagcct gagtttatcc tgaatcttac ctacaggcag gggcctgaga ttccttccct
     3601 tgaaggagcc tgcggctttg cagattcgaa cttcatctgc ttccagtatg agacgagaag
     3661 gaccggcatc tatgccgcgg ggagcgtaaa acagcccatg aacatgtcag aggcacagga
     3721 agatgcggca ggcgctgcct ttaaggcaat acagtgtatg gaccatattg ccaagggaat
     3781 ggcagttcat ccccgtgcgt gggatttgac cttccctgac cccatgatga ttaaatgcac
     3841 atcctgcaag agatgcacag aggaatgtcc ttttggagcg attgatgagg atgaaaaagg
     3901 gacgccgttt tataggctca accgctgccg ccgctgtgga acctgtatgg gtgcatgtcc
     3961 tgaaaggatt gtgcacttca aggacttcag tgttgatatc ataggttcga tgatcaagaa
     4021 gatagactca ccggatgaag agacattcag aattcttgtg ctggtctgcg aaaacgatgc
     4081 ataccctgcc ctcgacacag ccgccatcaa acggaaggcg atcgatccga gcgtgaggtt
     4141 tatttccctg cggtgtctcg ggggtatgaa ccttgtctgg atcgccgatg cgctttccaa
     4201 aggcattgac ggcgtacttc tccttgggtg caagtatggt gaaaactacc agtgccactt
     4261 tgtgaaggga agcgagcttg caaactaccg cttcagcaag gtgagcgaga ccctgaacag
     4321 gctccagctc gaggccgaaa gggtacagat gatgcaggtc gcaatcaacg agtatgacaa
     4381 actccccgac atgatcaacg agtttgtcga aaaggtcaaa gaaataggtc ctaatccgtt
     4441 taagggaatg taatataagt tcatggctca tagttcatag cttatggcgc taccagctgt
     4501 caactatcag ctgagaacta agatggaggt aaataaatgg cggaacaggt aataagtcct
     4561 gacgtaaatt tcgtcaagga tgtcatcagg aatggaggag aatcgctgaa aaagtgtttt
     4621 cagtgcgcta cgtgctcggt ggtgtgcaac gtgacgcccg agaacaagcc tttcccacgg
     4681 aaggagatga ttctcgcaca gtggggacag aaggaacagc ttatcagcaa ccctgacata
     4741 tggctctgcc atcaatgcag tgactgcacc gcctattgtc ccaggggggc aaaaccgggt
     4801 gaggtgctgg gtgcgatacg gaagataacc ataaaggaat tttctgcccc ttctttgctt
     4861 gccaatatgg tgagcgatgc caagtatctt attctcctct tcgccctgcc ggtgcttatt
     4921 ctcctcggtg tcacctctta ccacgggacc ctcaccgagg tcccgcgtgt ggacggcaag
     4981 atcgtctatt tcaagttcat cccgattcat tttggcgagt ttggcgttga tctcatcttt
     5041 accgctgctg cggtcttcgc tgccctctcg ttcttctttg gggtgaagaa gtactgggca
     5101 gccatgagca aaggggcgca gctcaaggga gacgttgtgg gaagcttcaa ggaagtaatc
     5161 tctgatatcc tttcccatga caggttcaag aagtgtaatg tgaccaaagc acggtcgacc
     5221 tcccatctcc ttgtttttta tgcctttatc ggactggcaa tcaccacaag ctgggcagtg
     5281 ttctacctct acattatgaa caggccctct ccctatccgc tttacgaccc gttaaaactg
     5341 ctcggcaacg tgagcgctct tgcgctgctt gcaggtatcg tgctggtgat caccaaccgt
     5401 ttaaaaaatc aggagaaggc ggggatcgga agctactatg actggctctt cattacagtg
     5461 gtcttcgcca ttatggcaac gggaatactc tctgaagtgt tcaggcttct tgacattgca
     5521 ccccttgcct atccgacata ctttacgcac cttgtctttg tcttttttct tttcgcgtac
     5581 gctccgtttt cgaagatggc gcatatggtc tacagggcca ctgctatggt cttcgcaata
     5641 gaagcgggaa gggagtaaca cagagttatg atttgcagag ggggatttct taagaaaatc
     5701 tatgtaaaag gaggaagatg atgagtctca cgattatttt tgcattaggg tgcggtctct
     5761 taggtgtgat atacgctg
//
LOCUS       DATKOU010000032        10102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4661524, whole genome shotgun sequence.
ACCESSION   DATKOU010000032 DATKOU010000000
VERSION     DATKOU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4661524"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="glyQ"
                     /locus_tag="VMU21_01260"
     CDS             <1..834
                     /gene="glyQ"
                     /locus_tag="VMU21_01260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HUO76180.1"
                     /translation="WSKRECVLLQPYDIEVGAGTFHPATFFRVLGPEPWKVAYVEPSR
                     RPTDGRYGENPNRLQQYYQYQVILKPSPDESQELYLDSLTHLGINPLKHDIRFVEDDW
                     ESPTLGAWGLGWEVWLDGMEITQFTYFQQVGGIDLKPVSLEITYGLERIAMYLQEVDN
                     VFNLDWGNGITYGDIHYITEVEGSKYNFDFADTDLLMRQFESYEREAKRLNELGLVTP
                     SYEYCLQCSHTFNLLDARGAFSVTERTGYIARVRGIAKLCAEAFFKQRENLGFPLLRN
                     T"
     gene            834..2966
                     /gene="glyS"
                     /locus_tag="VMU21_01265"
     CDS             834..2966
                     /gene="glyS"
                     /locus_tag="VMU21_01265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HUO76181.1"
                     /translation="MSDSPFPIASHSAEHSNQNTAPISDSSLLLEIGTEEIPARFIPP
                     ALIMLKGNTESIFEEYSIGFSDMKTYATPRRLALLVKGIPPVQKDRVKEIYGPAKKAA
                     FDEHGTPTRAAVGFAQSQGIGVNDLVVRNKEKGEYVVAVIHHKGAPVIDLLPEALKKI
                     ILSLNFPKSMRWGNGDLRFVRPIHWITALLDGTTIAFEIDGVKSANITKGHRFLSPGS
                     FIVKEIPSYVNLLENNYVIADIEERKRRIVEGIESLAASVQGRALRDEELITTVTNLV
                     EYPVPVLCEFPPDYLGLPKELLTTVMKDHQKYFALKNDHGELKNYFVVISNTKHENAE
                     TIKKGAERVIKARFEDARFYYEEDIKKTLHSRIQDLKRVAFHDKLGSLYDKTRRVVTL
                     ASFLSETLLPHLRQIIERAAWLCKTDLITGVVREFPELQGLIGKYYAAHDGEEHEVAE
                     AIMQQYLPSHSGGRLPESDVGALLSLSDKIDNIVSFFAVDLIPTGSEDPFALRRQALA
                     VIAILMEKGYPATLRELISKAEENVLDRRPSLPDEVFRFFAQRIEPLFSSQHYESDAI
                     QSVIPLMDNVPISEMKERMDALKKFTSDAEYTPFLLAMKRIHNIVPKTDVPALTPELL
                     LEEQEKSLCGELQNVKSTFNGLFRNKQFYDALKRLSTLTGTINNFFDKVLVMDKREEI
                     KLNRFALLQEIGKMTSLIADFSKLKETR"
     gene            3211..3861
                     /locus_tag="VMU21_01270"
     CDS             3211..3861
                     /locus_tag="VMU21_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUO76182.1"
                     /translation="MDTTLNDNPTRDKILLTLKKKGSMSVDGLSREVNITPMGIRQHL
                     LVLERKGIVEYVTKKHGVGRPGFLYRLTETADELFPNGYQDLALDILSDLERTEGKNK
                     IDGIFRRRKERLAAELTPQLEGMDTLSARVHTFSDILQKNGYIIELEDHPTVFRLKQF
                     NCPILKVARRFKEACDHDLQLYRELIGEAVRRERCISDGDHSCEYVIPKSQEGFSY"
     gene            complement(4000..4932)
                     /locus_tag="VMU21_01275"
     CDS             complement(4000..4932)
                     /locus_tag="VMU21_01275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUO76183.1"
                     /translation="MMKNIFVSVSLLLVSAALLFSCTKQGGQKSSAYIAKVGSEVITQ
                     EDVNRELKGLPPQIQNMFSGPEGAEKFVDELVKRDMLSQEAKKKGFENDAEYKKKVEE
                     FKKLTLISLLLQKEVEEKAKVTDKDVKDYYEAHKAELTTNNQVRASHILVKNEEEAKK
                     ILEQVKKGADFAKIAKEKSVDAGSAKNGGDLGFFSRGQMVPEFENAAFALKQGEVGGP
                     VKTQFGYHIIKVTGKKEGKIVEFEKVKDLLTQKVTAEKQKQVFDSYMNELKKSYKVEI
                     NKDALAKMWKDTKDKTGGANEKSGQPTAKPESAK"
     gene            complement(5256..6092)
                     /locus_tag="VMU21_01280"
     CDS             complement(5256..6092)
                     /locus_tag="VMU21_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HUO76184.1"
                     /translation="MASLKRILDRFYREYPFKERILHDPINFPHRYKQPRDIEISGFI
                     ASCLAYGRVELFTPVIDKILSLMGANPHAFLMDFNVRKHRVKFSFKYRFNEQEDILCL
                     VLILYELLKRYSSLENAFMRYYSPRDPHTGGGIDGLVREILSVDTTRVYGSNIHPRGL
                     TQMFPSPAKGSACKRMNLFLRWMVRDRDIDFGLWKGIPINKLVIPLDTHITRISRCLG
                     FTRRVSVDWKAALEITEALREFDSEDPLRYDFALCHHGISGICKTERNAGCRECAFYG
                     AK"
     gene            complement(6108..8225)
                     /gene="rnr"
                     /locus_tag="VMU21_01285"
     CDS             complement(6108..8225)
                     /gene="rnr"
                     /locus_tag="VMU21_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HUO76185.1"
                     /translation="MINKESILLFFREKTRKPLGYKDIVSLMKLSHPEARALKRLLRQ
                     MTREGHIVMTRKGLYGPAEDMELVTGNFEAHRDGYGFVILEKPGERDIFIPGRWTMGA
                     MNNDRVVVRMEHWQRREGRVIRILDRATTRIVGRLDITKTASFVRPINKATPFDLYVP
                     PQETGNAQHGDKVVAEITSFSTDTRPPLGRVIKIMGKPEDPRSDVEAIIEEFNLPKKF
                     PREVHEESKLLTQRAFLDAMGKRKDLKGLTTVTIDGERAKDFDDAVSIILTEHGYRLW
                     VHIADVGSYVGWNSAIDLEARERGTSIYFPDRVIPMLPKELSEDLCSLKPGVERLSFT
                     VEMEFDRCGERVSKKFYPSVIMSDERMTYTSVRKILVDDDMHERERYGSLLRDFELMN
                     ELCGILRKRRLERGSLDFDLPEPEVLLDLQGMPEAIIRAERNLAHMIIEEFMIAANEA
                     VAEHLESLGIPSLYRIHEEPDPMKLEDIVKVIRPTVKLKKKSLKPEDFSRLLADIKGI
                     PGEEIIHYMILRSLKQARYSPTNVGHFGLSSKSYTHFTSPIRRYPDLVVHRILKEVLI
                     KKHLSDKRIKELEGLLPEIAFHSSRMERRADEAEREVLDAMRVWFMKDRVGEEYEGMV
                     VGVTPYGLRLRLKDFYVEGFIHVSYMTDDFYRYNERAVSLSGRHTGRSFRIGDIVKVR
                     VDKIDMEEREILFGILGSPSKRK"
     gene            complement(8271..8456)
                     /locus_tag="VMU21_01290"
     CDS             complement(8271..8456)
                     /locus_tag="VMU21_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO76186.1"
                     /translation="MPYIDYSACTGCGACAELYPLFFVIRNERAWLINHEQFVFEKHG
                     RVVYCCPFGAITIESYE"
     gene            complement(8505..8957)
                     /locus_tag="VMU21_01295"
     CDS             complement(8505..8957)
                     /locus_tag="VMU21_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUO76187.1"
                     /translation="MQRVKKGDTVIISYIGRLEDGTVFDSTENRNPLEFTVGEGELIP
                     GLEQGIIGMMPGESKTILIPMEQGYGPHLNERVCELDKKRVPQGIQPEVGQQLTLYRA
                     DGLPIMGTVIGESGTSFTMDYNHPLAGKTLIFETRLLEIILEPPGQSF"
     gene            complement(9010..9813)
                     /gene="folP"
                     /locus_tag="VMU21_01300"
     CDS             complement(9010..9813)
                     /gene="folP"
                     /locus_tag="VMU21_01300"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HUO76188.1"
                     /translation="MGILNITPDSFTDGGLFFRRDSAIEHGLRMVEEGADIIDVGGES
                     TRPGSEPVEYEEEVRRIIPVIDALAKRIKVPISIDTYKADVAKRALDAGASIINDISG
                     LRFDPGMPKVAAECRVPIVLMHIKGTPKNMQVNPEYEALIPEIVDYLRISIKLAIDSG
                     VREEMIIVDPGIGFGKTFEHNLEILRNLHEFTLLEKPLLVGPSRKAFIGKILGNASSS
                     ERMEGTAAAVAISIMNGANIVRVHDVEEMGRVARVADAIKSGRIPKSSN"
BASE COUNT         2528 a         2651 c         2289 g         2634 t
ORIGIN      
        1 tggtcaaaga gggagtgtgt gctcctccag ccatatgaca tagaagtggg tgcagggacc
       61 tttcatcctg ccaccttctt cagagttctc ggtccggagc catggaaagt cgcctacgtt
      121 gagccctcac ggaggcccac agacggaaga tatggggaaa atcccaacag actgcagcaa
      181 tactaccagt accaggtgat actcaaacct tcaccagatg aaagtcagga actctacctc
      241 gacagcctca cccatctcgg cataaacccc ctgaaacatg acatacgctt cgtcgaggat
      301 gactgggaat ccccgaccct tggggcatgg ggactgggat gggaggtctg gctcgacggc
      361 atggagatta cccagttcac ctatttccag caggtggggg ggatcgacct taaaccggta
      421 tctctggaaa tcacctacgg actcgaaagg atcgccatgt atctgcagga agttgataat
      481 gtattcaatc tcgactgggg gaatggcatc acgtatgggg atatccatta tattaccgag
      541 gtggaaggct ccaagtataa ctttgacttc gcagacacag acctccttat gcggcagttt
      601 gaatcatatg aacgagaggc gaagcggctc aatgaactgg ggctcgtcac gccatcctat
      661 gaatactgtc ttcaatgctc ccatacgttt aaccttttgg atgcgcgagg tgcattctcg
      721 gtgacagaga ggacaggata tatcgcgcgt gtgaggggca ttgcaaagct ctgcgcagaa
      781 gccttcttta aacagaggga aaacctggga ttcccgctct taaggaacac ataatgagcg
      841 actctccctt ccccatagcc tcccacagcg ctgaacattc taatcaaaat actgcgccga
      901 tttccgattc atcccttctg ctcgagatcg gcactgagga gattccggcg agattcattc
      961 ctcctgcgtt gattatgctc aagggcaata ctgaatccat cttcgaagag tattccatcg
     1021 gtttttctga tatgaaaacg tatgccaccc cgagacgtct cgccctgctg gtgaagggaa
     1081 tcccccccgt gcaaaaagat agggtaaagg agatatacgg acctgcgaaa aaggccgcat
     1141 ttgatgaaca cggcactccc accagagcag ccgtgggttt tgcacagtca cagggaatag
     1201 gggtaaacga tcttgtggta agaaacaagg aaaaggggga atatgtggtg gcggtaatcc
     1261 accataaggg tgctccggtc atagacctgc tccctgaggc attgaagaag attatactct
     1321 cattgaattt cccaaaatcc atgaggtggg gtaacggaga tctcagattc gtgaggccga
     1381 tacactggat cacggcactc cttgatggta cgacaattgc tttcgaaatc gacggcgtaa
     1441 agagcgccaa cattacgaaa gggcaccgat ttctttcccc gggctccttt atcgtaaagg
     1501 agatcccttc ctatgtcaat ctcctcgaaa acaactatgt gatcgcggac atcgaagaga
     1561 ggaaacggag aatagtggag ggcattgaga gcctcgccgc atcggtgcag ggaagggcct
     1621 taagagatga agaactcatc accacggtaa caaaccttgt ggagtatcct gtgccggttc
     1681 tctgcgaatt tcctcctgat tatctcgggc ttcccaagga gttgctcact acggtcatga
     1741 aggatcatca gaagtatttt gccttaaaaa acgaccacgg agaactcaag aactacttcg
     1801 tcgtcatcag caacacaaag cacgagaacg cagagaccat aaagaaaggc gcagaacgtg
     1861 taataaaggc acggttcgag gatgcccggt tctattatga ggaagacatt aaaaagaccc
     1921 ttcattccag aattcaggac ctcaaaaggg tagcgttcca tgataaactc gggagccttt
     1981 atgataagac ccggagggtt gtcaccctgg catcgttcct gtccgaaacc cttttgcccc
     2041 acctaaggca aattatcgag agggccgcgt ggctctgtaa gaccgatctc attacaggtg
     2101 tcgtgaggga attccctgag ctccagggac tcatagggaa atactatgcc gcgcatgacg
     2161 gggaggagca cgaggttgcc gaggcaatca tgcaacagta tctgccctca cattcagggg
     2221 gcaggctgcc agaaagcgat gtgggcgccc tcctcagcct ttccgataaa atcgataata
     2281 tcgtttcatt ctttgccgta gatcttattc ccacaggatc cgaggatcct ttcgccctga
     2341 ggagacaggc actggcggtg atcgccatac tcatggaaaa gggatatccc gctaccttga
     2401 gagaactcat ctcaaaggct gaagaaaatg tgttggacag gcggccttct cttcccgatg
     2461 aagtgttccg cttcttcgcg caaaggattg aaccactctt ttcatcacag cactatgaga
     2521 gtgacgccat tcagtcagtc atacctctta tggataatgt acctatcagc gagatgaagg
     2581 agagaatgga tgccctcaag aaattcacct ctgacgccga atatacacca ttcctcctcg
     2641 ccatgaagcg catacacaat atcgtcccaa agacagacgt gccggcgctg acacctgagc
     2701 ttctgttgga agaacaggaa aagagccttt gtggtgaact gcagaatgtt aaatccacat
     2761 tcaatggtct gttcaggaac aagcaatttt atgatgccct aaagcgctta tccacgctga
     2821 caggaaccat caacaatttc ttcgataagg tcctggtgat ggacaagagg gaggagatta
     2881 agctgaacag atttgcactt ctgcaggaaa ttgggaaaat gacctcgctc attgccgatt
     2941 tctcaaagct caaagagacc agataacacc gacactcgat attcgagcat gaatatctcg
     3001 ggttacaatc gaaaaaccct gtcctccgtt ttccttagta gcgggatatg aatgcaagct
     3061 catgggatgg tgggtaaagc ggtcacccct caattacttc ccacattgac tgtaggaaga
     3121 aactctttca agcaagcgca tcccgagccc gctcaatcca cccaaaacag tctatttaca
     3181 aatatatgta attatgttat ttttttgcgt atggacacaa cccttaacga taatcccacg
     3241 agggacaaaa tactccttac cctcaaaaag aaaggcagca tgtcagtgga cggactgagc
     3301 agggaggtca atataacccc catgggcatc aggcagcatc ttcttgttct tgagagaaaa
     3361 ggaatagtag aatatgtgac caaaaaacac ggcgtgggaa ggcccggttt cctctacagg
     3421 ctgacagaaa cggcagatga gctttttccc aatggatatc aagacctggc gctggacata
     3481 ctcagcgacc tggaaaggac cgagggaaag aacaaaatag acggaatatt cagaaggaga
     3541 aaagagagac tcgccgcgga attgactccg caacttgagg gcatggacac gctctctgcc
     3601 cgtgtccata ccttttcaga catactccag aagaacggtt acatcatcga gctggaggac
     3661 catcctaccg tcttcagact taaacagttc aattgcccca tcctgaaggt tgcacgtcgt
     3721 tttaaagaag catgcgacca cgacctccag ctttacaggg agctcatcgg agaggccgtg
     3781 agaagggaac ggtgcatctc cgatggtgat cattcatgtg agtacgtgat ccctaagagt
     3841 caggaaggtt tttcttactg aaacgcatag cgagcgcatt ccccgttacg atctatcggg
     3901 actgtggggt taacgagcga atatgagaga gagcaattcc ttacgttaag ataccctgtg
     3961 gccccgagcg tgtttgggac cgcagggttc ttcatctccc tatttcgcgc tctcaggttt
     4021 tgcggttggt tgtcctgatt tttcatttgc cccgcctgtc ttgtctttag tgtctttcca
     4081 catctttgcg agcgcatcct tgttgatctc gaccttatat gacttcttca gttcattcat
     4141 ataagagtca aatacctgct tctgcttctc tgcagtaacc ttctgggtga ggaggtcctt
     4201 caccttttcg aactccacga tctttccctc tttcttcccg gtcaccttta taatgtggta
     4261 gccgaactgg gtcttcacag gcccccccac ctctccctgt ttaagggcaa aggcagcatt
     4321 ctcaaattca ggcaccattt gtccccgtga aaagaaccca aggtctccgc cattctttgc
     4381 agagccggcg tctacggatt tctcctttgc aatctttgca aagtcggcgc ccttcttcac
     4441 ctgctccaga atcttctttg cctcttcttc attctttacc agaatatggc tcgcccttac
     4501 ctgattgttc gtggtcagtt cggccttgtg ggcttcgtaa taatccttca cgtccttgtc
     4561 ggtcaccttt gccttctcct ccacctcttt ttggagaaga agactgatga gggtgagttt
     4621 cttgaactct tccaccttct tcttgtattc agcatcattt tcaaaaccct tcttcttcgc
     4681 ctcctgggaa agcatatccc tctttacaag ttcatccaca aacttttcag caccctcggg
     4741 tcctgaaaac atattctgaa tctgcggggg aagcccttta agctccctgt tcacatcttc
     4801 ctgggtgatg acctctgatc caacctttgc aatatacgcc gaagacttct gtcctccctg
     4861 cttcgtgcag gagaataaca aggcagccga tacaagcagc agggacacag acacaaatat
     4921 gtttttcatc atttcctcca atttcgtttc gtcttttata ccatatccta tagggtgatg
     4981 taaacacaaa tgcccggcat gatctcttct tcgcttcact gagacctctt tccgaaaact
     5041 tccccccaaa aaaacaacca agaaaaggct tgacagaggt cacgaaaaag agtagcctgc
     5101 agttaagagt gagaatttca ataggtggtg gtcttgatcc tttttacacg gggttccagg
     5161 cggtaaggtc ttagaggcga ggccataaga caatgggtcg tgagacgatt agtctctaag
     5221 ggactcgtgg ttaagaaggt accaagcacc accttttatt ttgcgccata aaaggcgcat
     5281 tccctgcatc cggcattcct ttccgttttg caaatccccg aaataccatg atggcaaagt
     5341 gcaaaatcgt atctcaacgg atcttcagaa tcaaattctc tcagggcttc agtaatttcc
     5401 agcgcggcct tccaatccac cgatacacgc ctggtgaatc ccaggcatcg tgatatcctt
     5461 gtgatatggg tatcgagagg gatcacgagc ttgtttatgg gtatcccctt ccataggcca
     5521 aaatcgatat ctctgtcccg caccatccat cgcagaaaga ggttcatcct cttacaggcg
     5581 cttccttttg caggtgaagg aaacatttga gtcaatcccc tgggatggat attgctcccg
     5641 taaacccttg tagtgtcaac agagaggatc tcacgcacaa gtccgtcgat cccccctcct
     5701 gtatgggggt cccggggact gtaatacctc atgaaggcat tttcaaggga tgaatatctt
     5761 ttaagcagtt catagagaat cagaacaagg caaaggatat cttcctgctc attaaaccgg
     5821 tacttgaacg agaatttcac cctgtgtttc cttacattaa aatccatgag aaaggcatgg
     5881 ggattcgccc ccatgagcga gagaatctta tcgatcaccg gcgtaaagag ctccaccctg
     5941 ccatatgcga ggcacgaagc gataaatcct gagatctcga tgtccctagg ctgcttgtag
     6001 cggtggggaa agttgatggg atcatggagg atacgctctt taaagggata ctcccggtaa
     6061 aatctgtcaa ggatacgttt taaagatgcc atcagatatc gcggcgatta tttcctttta
     6121 gacggtgatc cgagtattcc aaagagtatc tccctctctt ccatatcgat cttatccacc
     6181 ctcaccttta ctatatctcc tatcctgaag gaacgtcccg tatgtctgcc ggagagggat
     6241 accgccctct cgttatatct gtagaaatca tcggtcatat aggatacgtg gataaacccc
     6301 tccacataaa aatcctttaa cctcagtctg agcccgtaag gggtaacccc caccaccatt
     6361 ccctcatatt cctcccccac cctgtctttc atgaaccata cccgcatggc atccagtact
     6421 tccctttctg cctcatccgc acgtcgctcc atacgggatg aatgaaaggc aatctctggc
     6481 agaagaccct ccagttcctt gattctcttg tcagagagat gtttcttgat caacacttct
     6541 tttaatatcc ggtggactac aagatcagga tatcgcctga tgggtgaggt gaagtgtgtg
     6601 taggattttg aggagaggcc gaaatgtcct acattggtgg gcgagtaccg tgcctgctta
     6661 agacttctca aaatcatata gtgaatgatt tcctcaccgg gtattccctt tatatctgcc
     6721 agcagccggg agaaatcctc cggttttaat gatttcttct tcaacttcac tgtgggtcta
     6781 atgaccttta caatatcttc cagcttcatg gggtcaggct cttcatgaat cctgtacagg
     6841 ctgggaatac caagagactc gaggtgctct gcaaccgctt cattggcagc gatcatgaat
     6901 tcctctatga tcatatgcgc gagattcctc tctgccctga taatggcttc gggcattccc
     6961 tgcaggtcca gcagcacctc cggctcgggg aggtcaaaat caaggcttcc ccgttcaagc
     7021 ctcctcttcc tgagaatccc gcacagctca ttcattagtt caaagtccct cagaagggaa
     7081 ccataccgtt cgcgctcgtg catgtcatca tcgacgagaa tcttcctcac cgaggtgtag
     7141 gtcatcctct catcactcat gatcacactt ggatagaatt tcttgctcac tctttccccg
     7201 caccggtcaa actccatctc gaccgtaaat gaaagccttt ccacccctgg cttaaggctg
     7261 cagaggtcct ctgataattc cttgggaagc atggggataa ccctgtcagg gaaatagatg
     7321 ctggtgccac gctcccgggc ctctaggtct attgccgaat tccatccgac ataggaaccg
     7381 acatccgcga tatgcaccca gagacggtat ccatgctcgg tcagtatgat tgagaccgca
     7441 tcatcaaagt ccttggccct ctcaccgtct atggtgaccg tggtgagacc cttcaggtcc
     7501 ttccttttcc ccatcgcatc aagaaaggct ctttgcgtga gcaatttgga ctcttcatga
     7561 acctcccggg gaaatttctt tggaagattg aattcttcga taatggcctc tacgtctgat
     7621 cgcgggtctt cgggcttccc cattatcttg atcacccgtc ccaggggggg tcttgtatct
     7681 gtagaaaaac tggttatctc tgcgaccacc ttgtctccat gttgtgcatt ccccgtctcc
     7741 tggggtggca cataaagatc aaaaggagtg gccttgttta tcggcctcac gaacgaagcg
     7801 gtttttgtga tatcaagcct tcccacaata cgtgtggtgg ccctgtcaag gatcctgatg
     7861 acccttccct ccctcctctg ccagtgctcc atcctcacga ctaccctgtc gttattcatt
     7921 gcacccatgg tccagcggcc aggaataaag atatccctct cccccggttt ttccagtatc
     7981 acaaacccgt agccgtctct gtgggcctcg aaattccctg tgacaagttc catgtcttca
     8041 gcaggaccat agaggccctt acgggtcatg acgatgtggc cttctctggt catctgcctc
     8101 aggagacgct tcaatgccct tgcctcggga tggctgagtt tcatcaagga cacaatgtcc
     8161 ttgtacccaa ggggcttcct cgtcttttcg cggaagaaca aaagtatgga ttctttattg
     8221 atcatggcag gaaaaagtaa atcaacctat gcaatgcatt tacaaagttc ttattcatac
     8281 gattctatgg taatggcacc aaagggacag cagtatacaa ctctcccgtg cttctcgaat
     8341 acaaactgtt catggtttat gagccatgcc ctttcattcc tgatcacaaa gaataaagga
     8401 tataattcag cacaggcccc gcagccggta caggcactgt aatcaatgta aggcatcaag
     8461 ggtgttcaca ggtcttcctg acgaattcac ggttttccgt gatactagaa actttgccca
     8521 ggtggttcca gaattatctc caacagtctc gtctcaaata tgagtgtctt acccgcgaga
     8581 ggatggttgt aatccatagt gaacgaggtt ccggattctc cgatcactgt ccccataatg
     8641 ggcagtccat ccgcccgata aagcgttaac tgttgcccca cttctggctg aattccttga
     8701 ggtactcttt ttttgtccag ttcacagacc ctctcgttca gatgcggccc gtatccctgc
     8761 tccatgggga tcaggatggt ctttgattcg cccggcatca ttccaatgat gccctgttca
     8821 agacccggaa tgagttcccc ttcgcctacg gtaaattcga gaggattcct gttttcggta
     8881 gagtcgaaaa ccgtgccgtc ctcaagcctt ccgatataac tgattataac ggtgtctcct
     8941 ttttttaccc tttgcatttt cttccctttt ctgtttttcc cctattatac ccattttgaa
     9001 agaagatttt taatttgaag attttgggat acgtccgctc ttaattgcat cagctactct
     9061 tgcgaccctc cccatctcct ccacatcgtg aactctcacg atattggcac cattcatgat
     9121 ggaaatggcc accgctgctg cggtcccttc catccgttcg gaggaagatg cattgccaag
     9181 gattttccct atgaacgcct ttctcgaagg gcccaccagc agtggttttt caaggagcgt
     9241 aaattcatgg aggtttctca atatctcaag gttatgctca aatgtcttgc caaagccgat
     9301 tcccgggtct acgataatca tctcttccct cacccctgaa tctatggcaa gttttatgct
     9361 gatcctcagg taatcaacaa tctcggggat cagcgcctca tattcaggat tgacctgcat
     9421 attcttcggc gttcccttga tatgcataag cactataggc acacgacatt ctgctgcgac
     9481 tttcggcatt cccgggtcaa atctcagccc gcttatgtcg ttgataatgg atgcccctgc
     9541 gtccagggcc ctttttgcca catcggcttt ataggtgtct atggaaatgg ggactttaat
     9601 tcgttttgcc agtgcgtcga ttacaggtat gatgcgccga acctcttcct catactccac
     9661 cggttcagag cctggccttg tggattcacc tcccacatca atgatatccg caccctcttc
     9721 caccattctg agaccgtgtt caatggctga atccctacgg aaaaaaagac ccccatccgt
     9781 gaaagaatcg ggagttatat tgagaattcc cataacatgg gtcttctgtg aaaaatcaag
     9841 ggagaattgt ctgaaaaaga gcttcacctc agcactgggc caccgaggca gaaggaaggg
     9901 ttcggcacat tgggcaggtc tcttgtgcta cacctggatc tcggccttct cgtcttcaga
     9961 agaggggctt ttgtcccctt taagtccctg gataagttcc tcaagttcgc ggccatccat
    10021 ggtctctctc tccagaaggg atttggcaaa ggaatctagt atatcataat tctcagagag
    10081 aagtttcctg gactcctcat ac
//
LOCUS       DATKOU010000033         4174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5430584, whole genome shotgun sequence.
ACCESSION   DATKOU010000033 DATKOU010000000
VERSION     DATKOU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5430584"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VMU21_01305"
     CDS             <1..815
                     /locus_tag="VMU21_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76189.1"
                     /translation="KDGFIVVGMGKIGGREITFDSDLDLIFACREDVQEKEVKAAERL
                     IRLLTSYTKDGLAYRVDVRLRPEGTKGPLISAMNALERYYSRDAHSWEIQALLKARPI
                     AGDPYTAQYFMEMREDILRRRGGEVSATDIKGMRERIQRELSREKEGYDIKLGPGGLE
                     ELEFAVQYLQLANADSHGDLLVQGTSDAIKRLASTDLIAGEEADFLRDSYLFYRKLES
                     FMRLKGEPILKKSGISTGDVEEFMGFGDSKELFAHVEERRTQVKKRFEKYLY"
     gene            857..1699
                     /locus_tag="VMU21_01310"
     CDS             857..1699
                     /locus_tag="VMU21_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HUO76190.1"
                     /translation="MLKLRDGIVELYKKVATSIPPDVEEAMKGAFSSEGDARGKKSLA
                     GILEIIRKSRQVSRPACIETGIPIFKVKVPRGLSHTEMKTTIREATAIATRRVPLGLS
                     AVDILTGENSGDNTGIGFPVIYLEEAPNDTLAVDLMLRAGDCENYGQTYRLPVEDLQA
                     GRDLDGVRRCVLDCVAKAGGKGCPPYTVGVGIGATHDQVSVLAIHQLFRKIPDESEYP
                     AIAGLEKRLFNEINELGIGPVGSGGTTTALGVKIGINHRHPESFLVHVALACWANRRG
                     RLIW"
     gene            2069..2485
                     /locus_tag="VMU21_01315"
     CDS             2069..2485
                     /locus_tag="VMU21_01315"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HUO76191.1"
                     /translation="MEEFEVIDISGDAGIRAYGETLEDVFTNAAMGMYSLITDLKGIG
                     EIKEIAVTAESRSLEGLVVSWLNELIFHFDTYGFIGRKIRVETLENHRIVAKISGEDF
                     DPERHERRLLIKAATYHQLSMEKKEGHWVANVIFDI"
     gene            2589..3950
                     /locus_tag="VMU21_01320"
                     /pseudo
     CDS             2589..3950
                     /locus_tag="VMU21_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(3978..>4174)
                     /locus_tag="VMU21_01325"
     CDS             complement(3978..>4174)
                     /locus_tag="VMU21_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76192.1"
                     /translation="RGTVIMHTNGIVSTNIGYFGATVRFNLDLDGDGIQDISPDYDCL
                     VLFSTRINIGTWQGGKCVAT"
BASE COUNT         1073 a          951 c         1252 g          898 t
ORIGIN      
        1 caaaggatgg gtttattgtt gtcggcatgg gtaaaattgg gggaagagag atcacctttg
       61 attccgacct ggacctgatc ttcgcgtgca gggaagatgt gcaggaaaaa gaggtgaagg
      121 ctgcagagcg gctcatcagg cttctcacct cctacacaaa agatgggttg gcataccggg
      181 tggatgtgag gctgcgtcct gaaggaacaa aaggcccttt gatctcggct atgaatgcat
      241 tggagcgcta ctattcccgc gatgcgcatt cctgggaaat ccaggccctc cttaaggcaa
      301 gacccattgc aggagatcct tacactgcgc agtattttat ggagatgagg gaagatatcc
      361 tcaggaggag agggggggag gtatctgcca ctgatatcaa aggaatgagg gaaagaatcc
      421 agcgggagct ctccagggag aaggaaggct atgacataaa gttgggtccc gggggactgg
      481 aggagcttga gttcgcggtc cagtatcttc agttggcaaa cgctgatagc cacggtgacc
      541 tcctggttca gggaacatca gacgcgataa aacggctggc ctccacagat cttatcgccg
      601 gggaagaggc tgatttcttg cgggacagct acctctttta ccggaagctg gagagtttca
      661 tgagattgaa gggtgagcct atcctgaaaa agtcagggat atccaccggg gatgttgagg
      721 aattcatggg ttttggtgac agcaaggagt tatttgcgca tgtggaagaa agaagaacac
      781 aggtaaaaaa gcggttcgag aaatatttat actgagcatg tgaacaagtg tgatataatt
      841 tttatgaggt ggacacgtgc taaaactccg tgacggtatt gtagagttat ataagaaggt
      901 ggcgacttcc attccccctg acgtggaaga ggcgatgaag ggcgcgtttt cctctgaagg
      961 agatgcgagg ggcaagaaat ctcttgcggg catactggaa atcatccgga aatcaaggca
     1021 ggtttccagg ccggcctgta tcgagaccgg catccctata ttcaaagtga aggttccccg
     1081 ggggttaagt cacacggaga tgaagaccac catacgggag gccactgcca ttgccacacg
     1141 cagggtgccg ctgggtctga gtgccgtgga catactcacg ggcgagaatt caggtgataa
     1201 caccggcatc ggtttcccgg tgatctatct tgaggaagca cccaatgaca cgctcgcagt
     1261 agacctcatg ctcagggcag gggattgcga gaactacgga cagacgtacc ggctgcctgt
     1321 ggaggacctg caggccgggc gtgaccttga cggggtcagg agatgcgtcc ttgattgtgt
     1381 cgcaaaggca gggggtaaag gctgtcctcc ttacactgtg ggggtgggga tcggtgctac
     1441 tcatgaccag gtctctgtcc ttgcaatcca ccagctcttc aggaagatcc cggatgagag
     1501 tgaatatccg gccattgcag ggctcgaaaa gagactcttt aatgagatta acgagctggg
     1561 aataggaccg gtgggcagtg gcggtaccac cactgcgctt ggggtgaaga taggcatcaa
     1621 ccacagacat cctgaatcat ttctcgtgca tgtcgctttg gcgtgctggg caaacaggag
     1681 aggccgcctc atctggtaac catccctagc ccatcaattc ataagctcct tcatggtaaa
     1741 aggctcaccc gctccaaggc aagatacaat agccctcatt gactataccg ggtctgcgca
     1801 tctaagaaca ttttgttccc attttttgtc acggtccgct cctgcgggcc ggcacataac
     1861 tcatccgcct caggcggatt gccgttcgag ccggacacct ctcccgagtg tatgaattat
     1921 ctgggctgga gtcccaaccc aaaatctctt ctccggacga tacgcccccg agaagaagcg
     1981 aatggtgata tcatgcgcag cttgcagatc aagactggct gttccgcctt aggttgacgg
     2041 ctatcctgtc cggtgatacc ctcttaatat ggaggagttc gaggtaatag acatctccgg
     2101 tgatgcaggg ataagggcat acggagaaac cctcgaagat gtctttacca atgcagccat
     2161 ggggatgtac agccttatca cagatcttaa aggaatagga gaaataaaag aaattgctgt
     2221 gaccgcagaa agccgttccc tggagggact cgttgtgtca tggctcaatg agcttatatt
     2281 tcactttgat acctatgggt tcattggcag gaagataagg gtggagacgc tggagaatca
     2341 ccggatcgtc gcaaagatat cgggtgagga cttcgatcct gaacgacatg aaaggaggct
     2401 gctcatcaag gcggctacct accaccaact cagcatggaa aaaaaagaag gacattgggt
     2461 tgccaatgtg atctttgata tctgaacaga agggaggtga tgttatgggt atcgagaagc
     2521 tcaggaggat cgactctacg aagttggaga tccccgcaga ttataaaccc ggcatgaggg
     2581 taaagggaat gatttatgtg gatgatgtcc ttgagaaggc ccttgagagc caagccattg
     2641 accaggtggc gaatgtggcc acgctgccgg gcattgtcag ggcgtccatg gccatgcccg
     2701 atatccatac cggctatggc tttaccatag ggggtgtggc agcttttgac gttaaggaag
     2761 ggatcatctc gcccggaggg gtgggctacg acattaattg aggggtccgc cttctcagga
     2821 gcaacctcag aaggggagag gttgttccga gaatgaagga cctcgtgggg gtcctttacc
     2881 gggaaattcc ttccggagtt ggttcaaagg ggaaaatccg cctcggtcct gaggaggaaa
     2941 ggaaactgct caccaaaggt gcacgatggg ctgtggaaaa agggtatgga gacgcctctg
     3001 atcttgagag gatcgagtca ggaggctgca tcgagggcgc tgaccctgac ctcataagtg
     3061 ataaggcata tgaaaggggg agggcacaac agggcaccct cggttcaggc aaccacttcc
     3121 tcgaaatcca gtatgtggat gagatatacg atgaagagtc agccaatgcc cttggtctct
     3181 ttaaagacca gatgaccgtg atgatccaca caggctcccg cggattcggg caccaggtct
     3241 gcacagattt cctcgaggta atggagaggg cggtgaacaa atacaagatc acactccctg
     3301 acagggaact tgcgtgctcg cccttgggaa gtcctgaggc ggctgattat ctcgctgcca
     3361 tgagggctgc agccaattat gcgtgggcaa acaggcagtg catcatgcac tggaccaggg
     3421 aggcttttct gggagttttt ggggccacgc caagagacct aggcatgagc ctcatctatg
     3481 atgttgcaca taacattgca aagatcgaag agcatacggt gaacggcagg agaatgaaac
     3541 tcgctgtcca caggaaaggg gccacacggg catttcctcc aggacatccg gagcttcccg
     3601 atgcatacaa aaagacaggg cagcctgtgt taatccccgg ggatatggga agggcctctt
     3661 ttgtcttgct ggggaccgaa aaggcgatgc aggagacctt tggctccacc tgccacggag
     3721 cggggaggat catgtcgcga caccaggcaa tacgccaggc aaagggcagg gcaatatggc
     3781 gggagatgga ggacaaaggg attatcgtca agtcagcagg gagggaaacc cttgcagagg
     3841 agatgtcaga ggcttacaag gacatatcca atgtggttga tgtggtgcat aatgcgggga
     3901 tatcaaagaa ggtcgcgcgg ctcagaccca tgggtgtggt gaagggatga ggtcagaggg
     3961 gagtggaatt ttcatcctta cgtcgcaaca cactttcctc cctgccatgt cccgatattg
     4021 attcgtgtgg aaaatagtac gaggcagtca taatccgggg agatatcttg gataccatca
     4081 ccatcaaggt cgaggttgaa gcgcaccgtg gccccgaagt atccaatatt ggtcgaaacg
     4141 atgccgtttg tatgcataat cactgtcccc ctcc
//
LOCUS       DATKOU010000034        19715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_817674, whole genome shotgun sequence.
ACCESSION   DATKOU010000034 DATKOU010000000
VERSION     DATKOU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 19715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19715
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817674"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="rpsB"
                     /locus_tag="VMU21_01330"
     CDS             complement(<1..679)
                     /gene="rpsB"
                     /locus_tag="VMU21_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HUO76193.1"
                     /translation="MVVTMKELLEAGVHFGHQVKRWNPKMKKFIFGERNGIYIIDLQK
                     TLKGLEDAYKFVRETSIGNSAILFVGTKKQAQDAIQEEAKRAGAFFVNQRWLGGMLTN
                     FTTIKKSIERLKSIEKMKEDGTYEALSKKEVSLLEKERTKLEKNLTGIKEMSGLPGAV
                     FIIDPKKEKIAIAEARKLSIPVVAVVDTNCDPDEVDYVIPGNDDAIRAIKLIASKMAD
                     SVIEGRDV"
     gene            1305..2303
                     /locus_tag="VMU21_01335"
     CDS             1305..2303
                     /locus_tag="VMU21_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HUO76194.1"
                     /translation="MTLLRSSFCFLTVLVLGLTGCEALKASKPIIPIKEYEQMIAGRL
                     DANYVGTRTCLSACHFHDKIRRDFEASTMGAQLSSKSGMPLVDCESCHGPGSLAIEGI
                     TPEKVAMDAKEGKQTACYYKTLIEIKNLPAGAQSLICLKCHTANATFNLHSWNASAHA
                     INDVSCSECHKVHVGPDLTVRRRAMAEMCDRCHQEVRAAFYLPSHHPVPEQKVFCNDC
                     HDPHGSFYTKSMKKGTIKETCSQCHMEKAGPFAFGHADNTEDCTTCHSPHGSVNNWLL
                     TLRVPFLCLQCHPGHRTSKTSSAESKGAFYTRCTDCHSTIHGTDLPSASGRGRFTQ"
     gene            2318..4318
                     /locus_tag="VMU21_01340"
     CDS             2318..4318
                     /locus_tag="VMU21_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76195.1"
                     /translation="MKKILSIALAVLVSMILVHMAYSQETAAPQVAQESGMEETAPSE
                     AYTFPTIKPEASLFLGGRLAGFSGSPRADQYEYLHDFVPFGGELRLFEYPHRLHLDVD
                     IQDQKDYFGDLSYSYKDLILFRGVDRTVWHNLVNIPLIDLNPATPSPGVDVRDSGVEY
                     GTRTSMNNASLRFKMPDFPLHIYLDGSYIDKSGGSMQQRFLGGSGYFNNIVRVSEQRD
                     VDWVTKGLLMGINSHLGPIEADFSHGERRFDSSGTQVFQDFYTDAGVRPAGVFEHNLI
                     PDFKSSSNTLKLHTSYTGGIVASATLSNIDTKNDSSGTKADYYIGTADFTWMPMPKLT
                     FYVKYRYKDTDFDNPGSVTIADLSNPFQTFTYPVKAAISSITNTVSGTVRYLPIKGVT
                     LRAEYAFQNIDRDNASLWGLPDSTQSNTVTISADTRILKGLNFKAQYTYLQISNPAYN
                     TDPNHSNSAKFSLSWIPIPQVNTYVSYTFAREKRDNVSFVEADGTITTTPNNRDVNKD
                     MVMGSATFLILKDLSLTGSYAYMHNNTEQDMGYSDLAGIPQTDPSVPNKQTAQTVAAD
                     LNYMPKNNITLHAGFSYTLSSGQFYPSSADLLQPVSVASFSALKEKETAYTLSGEYRF
                     KNGFGLELRYKYCHLNDVQNNPYDDVQNGNANIFMVLLSKRW"
     gene            4323..5312
                     /locus_tag="VMU21_01345"
     CDS             4323..5312
                     /locus_tag="VMU21_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76196.1"
                     /translation="MMNKVKKSRILACALRERTPFAVLLYVWTMVVLASMCFSPPTFA
                     AEKTVGVIMTGNISYYKDIHKAFIEGLNQEGLFAQGKVDVVVQTPAPETMAWTNAARK
                     LVAIGSAVIVSYGAPATLSVMSETSDIPIVFAGVYDPQALGITGKNITGISAKVPVTS
                     LIKNLKSISNFKTLGVIINDSEKDSVLQANEVKQLEGSFGFSSKSFNVKRAEDASRIT
                     GVDALYLTTGCTGMHCVTNIVGVARKGKMASGATIGGGEASGVILTIAANPQEQGREA
                     AERVAKLIKGASAASLPVEQPKKVDMIINLKEATDMGLKVPFDLLTSATKVIK"
     gene            5309..7384
                     /locus_tag="VMU21_01350"
     CDS             5309..7384
                     /locus_tag="VMU21_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUO76197.1"
                     /translation="MNRGINLQKKAIIMVGGILFFILAINTLVLTSVASNKYKSALLS
                     KTTAVGEGMQRDLGRALNLGIPLESMEGVNEKLQELISRDPAIGYTMVTDIKGKVFFD
                     TRQAELGKELKDDASLKAASSPKSIIQTIGAFYDLSFPLMNAEGKMVGVLRIGIKSEA
                     VNSQLYALLLWALGISLVSFLVSLGLVSFSISRFITKPIMHMEKAAEGIAAGDLTSVI
                     ETKGSDEIAALGKAINGMAFNLKDMLSKIRNISNSVTHVTSNIVNSSQGVLAVADVQK
                     KAIEETAVAMTEMNSSTSSVAQSAESLSDSSVDTSSAILQMTKSIERVAESSNIFDEQ
                     THEAASSIEEMISNIKQITQSLENLSSSSEEIASSVTEVDATIKEIEHHAGESVRLAE
                     KVTTDASDKGMNAAEAAIEGMENIRKSVGALSDMINILGKRSEDIGKVLNVIDEVADQ
                     TNLLALNAAILAAQAGEHGRAFAVVADEVKSLAERTSLSTKEIAALITSVQEDTRSSV
                     QMAGQGIETVERGLGLVRDVHEALSGILDSSRVSTEMSKAIQRATSEESQVIKQITDS
                     IKGMSKQVENISRAAQEQSKGSKFIVEATEKMREISHHIKVATGEQKDGSRQISGAVE
                     NVTQQADHIAKATGKQLEKSAEIVQSMDKIQNTTGKLIQVSHEMSAAINSLKEEARNL
                     LSELQKFNV"
     gene            7449..8561
                     /locus_tag="VMU21_01355"
     CDS             7449..8561
                     /locus_tag="VMU21_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO76198.1"
                     /translation="MKIQPPIIGSKGIGSSRAWDLETLINSLSEAILLLDKRNTIVFV
                     NKTGEEFFGKSHQEIIGARLTDLYPGAKTIARLARKAMKELRSFSAKDVEAKINGISS
                     VDIDITPYLTDDKCSGVLLSIRENLSIAEREDSSFDSLVYLLGTIAHEIKNPLGGIKG
                     AAQLLKGKLLKGEARAFAGADTVDEYINLIIRETDRLNTVLQNYLTMSRRPALQEMNI
                     HEILEKAISIMDVLLKNVNIGIQRAYDPSLPRVIGDGGKLLQVFLNVIKNGIEAMPRG
                     GNLSIATKPSAEYAVQHGQIKRWAVVSIRDTGKGIPKQEMQKIFLPFYTKKKKGTGLG
                     LALSKKIIKDHNGFIRVERPESGKGTVFHIYIPFAQ"
     gene            8594..9985
                     /locus_tag="VMU21_01360"
     CDS             8594..9985
                     /locus_tag="VMU21_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO76199.1"
                     /translation="MGKEVLIIDDDEAITWVVKKALEPLGYKVVTRPRLSSGLKAVKG
                     GLQLVLLDIVLPDGNGLNALKEIRSTFPDAMVIMVTAHGRMESTVEAMREGAYDYIEK
                     PFDVEELKINVEKAFRDMAVREELSNLRSVTAEAEEPLIVGRSPKMLKVFKEIGRVAS
                     KDITILIVGESGTGKELVAKAIHHNSNRRTGPFVAINAASIPKDLLEAELFGWEKGAF
                     TGAKEKYAGKVESARGGILFLDEISELNLDLQAKLLRFMQDKKFSPLGSNTVMEGNVR
                     ILGATNRDLKEAITKGLFREDLYYRFNVVQIKLPTLAERREDIIPLAKHFLKDAERKF
                     ETGKKELSREAKDLLLKYPWPGNVRELENAIKRACILSNGTVIEKRDLPLEEANSFSI
                     QDFLEEKLKRYLKEMTKLEKCNLYDSVVSEVEKALIKIVLNETGGNQLKASKTLGINR
                     NTLRTKIRGYKIK"
     gene            10131..10529
                     /gene="rpsF"
                     /locus_tag="VMU21_01365"
     CDS             10131..10529
                     /gene="rpsF"
                     /locus_tag="VMU21_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HUO76200.1"
                     /translation="MNIYENIVILNASPADEEIEASLNKIKDLITNAGGEILKVDAWG
                     RRKLANEIKKQKKGFYVLLVFRSASSLVRKLEDYYKVFDPVIKYMVIKLGSKESTHFL
                     ATLIQTPRGDEATAKPAPGTATPAPGAASE"
     gene            10556..10951
                     /gene="ssb"
                     /locus_tag="VMU21_01370"
     CDS             10556..10951
                     /gene="ssb"
                     /locus_tag="VMU21_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HUO76201.1"
                     /translation="MYNRIILIGNLTKDPELRYTPQGTPVASFRIAVNTRYKQSDESR
                     EDTLFIDIVTFGKQAETCSQYLNKGKSVLVEGRLQERRWESEGQQKSKFEVIAQTVRF
                     LSRKGAGEGQGVPESEVPPPEGMTDPEPF"
     gene            11013..11237
                     /gene="rpsR"
                     /locus_tag="VMU21_01375"
     CDS             11013..11237
                     /gene="rpsR"
                     /locus_tag="VMU21_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HUO76202.1"
                     /translation="MQQRRFQRRKFCRFCAEKFEFIDYKDIKTLRGYITERGKILPRR
                     MTGVCARHQRSLNEAIKRARNIALLSFVEK"
     gene            11238..11519
                     /locus_tag="VMU21_01380"
     CDS             11238..11519
                     /locus_tag="VMU21_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HUO76203.1"
                     /translation="MRIPRTWVPLIAKRVLDDLIAKGLVISKVSTEVLMAEAERLIME
                     ELIIEDRINDEVREILKKYTGEMQKGRLDYRKMFELTKKKIVQERGVIL"
     gene            11519..11800
                     /locus_tag="VMU21_01385"
     CDS             11519..11800
                     /locus_tag="VMU21_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HUO76204.1"
                     /translation="MLSDEKVSHLTHLLFKGLTDKALIELVAEEGKVRREVKRSIVSF
                     LKVSEEIDLSVRKKLQSFSRKIVEGSPEWEVLYKKFYREEAAKRGLASE"
     gene            11812..13737
                     /locus_tag="VMU21_01390"
     CDS             11812..13737
                     /locus_tag="VMU21_01390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUO76205.1"
                     /translation="MPFLLLLSYSGLIFNFPSTRPSVWFCFSLFPVAAYVFLCFIQRQ
                     KKYTFETLASLVLLMAGGIQFLRHDWLHGLYVPCLVIFAFFYGPRVLIPLSITIPFLE
                     IRHFVQGNPMEEAFSSGAAALSGILVSLVVSRTREERDRLKKSFDALKEEAENMDPVT
                     TAGITSTNGLASQHLSSARRTNEEIREVLLIAKHALTADSAQMFSLEEKSLHPRCSAD
                     ESSSVDIPDSGPLLLCLQKRQSLVFTSGAHKNTPLLSNIAVPIVDGSFAYGVLTLQGR
                     KGVLKENDPKMVEMFSRQIVNILNRQRIYSQLQKEHLMLRKLKDGGTRMISSLKMDDI
                     APSLIDAVYSIAPQERVSIALFVPKEEQFEMVRQIGFALSEGSTFDLGSTRIGLVGRS
                     REPDYISDLRNERSQALPFKIPDEGSLFMMPLSYEKELLGILVFLSPIPNAIRPYQIE
                     LLKVLGNQAAASLANAKFHAEIERMAITDGLTGLFNHRNFQEKLTAEFRRLERFSASL
                     SLLLIDIDFFKKINDSYGHPAGDEVLREVSRIIRETIRSVDIPARYGGEEFAALLPGT
                     NHEGALKMAERLRESIEKTNFPIEGKKLRVSVSIGAATSPHDAAAKEELVEKADKALY
                     YAKRNGRNRCVLWREVK"
     gene            13738..14040
                     /locus_tag="VMU21_01395"
     CDS             13738..14040
                     /locus_tag="VMU21_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUO76206.1"
                     /translation="MEPFISYVTEEEIRRARARARELRKTQWWKRKCAAGICHYCKKK
                     VNPRELTMDHIVPVIRGGKSTKGNLAPVCKECNDKKKYLLPIEWEEYLERLGKEEI"
     gene            14088..14459
                     /locus_tag="VMU21_01400"
     CDS             14088..14459
                     /locus_tag="VMU21_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HUO76207.1"
                     /translation="MDIFAKIAEERIRQAMEDGEFASLPGSGKPLMLEDETWIPEDMR
                     LSYRVLKNAGCIPPELEKRKEILNLKELLNTIDDDKERLRKLRELNFKLMEFNIIKGR
                     PLYLETFPDYEQRIFEKITNS"
     gene            14506..14985
                     /locus_tag="VMU21_01405"
     CDS             14506..14985
                     /locus_tag="VMU21_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HUO76208.1"
                     /translation="MGEDTRSTIVLVVCCTLIIAAAAALGWSGDDILGLWNNEERDGI
                     IEIFKCREDYCGRIVWAKEPTYPADDKMGRAGQPRLDDNNPSPGLRTRPIIGLQIMND
                     FVFDGDNGWKEGTVYDPKNGRTYSGKVTLVSPNELRLRGYVLFSFFGRTTIWTRLEH"
     gene            complement(14995..16068)
                     /locus_tag="VMU21_01410"
     CDS             complement(14995..16068)
                     /locus_tag="VMU21_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HUO76209.1"
                     /translation="MRNKEQMSGNVYVGSIAVETPLFTVQQEEVGKILAHRYSDRLSS
                     RSLTLMKGLFAHPAIRKRRFAVDDSEGFFSENPDDRIQRFTRWAVDLSAQAIRNALVK
                     AGLSFDDMAGLVVNTCTGYICPGISTYLIEKLGLDRHIRVYDLVGSGCGGAIPNLEVA
                     ESLVRGASHKVIVSVSVEICTATFQMEDDLSLLVSNALFSDGAAAAVLWGRPQGIELV
                     ASSSYYVPEDRDAIRYIHKNGQLYNQLSTRLPHLVKKAVAQVVKDVLEPRALRVGEIK
                     HWALHTGGEKIINTVKAEIGLSEEQLRSTRAILAEYGNMSSPTVWFVLNDLLEKGIER
                     GDFCVMVAFGAGLSAHAYLLRKS"
     gene            complement(16070..17497)
                     /locus_tag="VMU21_01415"
     CDS             complement(16070..17497)
                     /locus_tag="VMU21_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76210.1"
                     /translation="MKVLIISTNSNQFPMPVMPLGACLVADAAARAGHEVRLLDLMFQ
                     QDPLRVVKRELEKRNPDIVGLSVRNIDNNDIQSPNFFLKDILPLIDSVRGMTSAPVAL
                     GGAAVSVMPEELLRYTGASWAVLGDGETMFPMLLEKLSQEEPPETLPGIAWIEKGTFN
                     VNPCASSMNGCSCASPDFRRWIDLKTYLRRLSTVPIQTKLGCQFSCVYCTYRKIEGSR
                     YRLSDPEEVVDTIGKLTAGGLTHIEFVDNVFNSPYDHALSICEKVARARSRVSLESLE
                     LNPLSIDDTLIAAMERAGFSGIGITVESASDSVLEGLGKGYTKEHVHKAAEVVRRHTL
                     PCVWIFMMGGPNETEGTVRETLRFAERCIRPTDTVFLGMGIRVYPGTGLEAIARKQGL
                     LSHSSAQMLEPVFYLSPTVEYNWMRKQIETSMNEHMNFMNSSSLSSPLLPSIHRLAYK
                     VGLRSPLWKHTSFIRRGLRLLGMEV"
     gene            17700..18101
                     /locus_tag="VMU21_01420"
     CDS             17700..18101
                     /locus_tag="VMU21_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HUO76211.1"
                     /translation="MDRKSKGLIAVFVTVLLAFAACASQQPLVTIGPARGEIVLAMKA
                     SDFTFEPNNIKAYKDDVVVLKIENISSTGHNFTLKTPLGNAEQSVKLPPMETVTVKVE
                     LSEAGTYEFYCDKPFHSTLGMKGQIVVTQGP"
     gene            18285..18992
                     /locus_tag="VMU21_01425"
     CDS             18285..18992
                     /locus_tag="VMU21_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HUO76212.1"
                     /translation="MRRKGQGLSFIGAFVTVLLFIFGVFFTAATPVMAADVNEAQGFV
                     DKARVTFNEFMRDSNYGWLHEHLKEAKGLLIFPQVIKGGFFLGGSGGTGVLVARDAKT
                     GDWSQPAFYTIGSVTFGLQIGGEAAEVIMMAMSQKAIDSLFASSFKLGGDTSITLGPV
                     GAGAKGNITADFISFAKSKGLYAGLNLEGSVVDVRDSLNRAYYGKEVRPVEIVVEKKV
                     SNKGSSELRTTLKKAVK"
     gene            19145..>19715
                     /locus_tag="VMU21_01430"
     CDS             19145..>19715
                     /locus_tag="VMU21_01430"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HUO76213.1"
                     /translation="MKLGRVFTHAVLWLLVALMAAPPWAFAQDTEGGPSAAFSREQLE
                     QILAPIALYPDELIAQILMASTYPLEVVQADRWVRQNNNLKGDALADALEEQDWDPSV
                     KSLENFPQVLQMMSEKLDWTQKLGDAFLAQEKDVMDTVQVLRRKAEAAGNLNTTQEQK
                     VIVEKETIIIEPANPQVVYVPTYNPTVVYG"
BASE COUNT         5310 a         4759 c         5180 g         4466 t
ORIGIN      
        1 ccacgtccct tccctcgatc accgagtccg ccatctttga cgcaatgagc tttatcgccc
       61 gtatcgcgtc gtcattcccc ggaatgacgt aatcgacctc atcagggtca caattcgtat
      121 caacaacggc aactacggga atagagagct ttctcgcctc tgcaatggcg atcttctcct
      181 tcttgggatc tattatgaat accgctccag ggagtcctga catctccttt atccccgtga
      241 gatttttttc gagcttagtc ctttccttct ccaagaggga aacttccttc ttggaaaggg
      301 cttcgtaggt gccgtcctcc ttcatttttt cgatactctt caggcgctcg atgcttttct
      361 ttatggtggt aaagttggtg agcattccgc caagccatct ctggttcacg aaaaaggcac
      421 ccgctctctt cgcctcctcc tgaatggcat cctgggcctg cttctttgtg cccacaaaaa
      481 gtatggccga gttacctatg gaggtttccc tgacaaactt atatgcatcc tcgagtccct
      541 tcagtgtttt ctggaggtca atgatataaa tgccgtttct ctctccgaag ataaattttt
      601 tcatcttcgg attccatctc ttgacctgat ggccgaaatg tacgccggcc tccaggagtt
      661 ccttcattgt tactaccata aaatgcgtcc tcctttggtt tttcctccgc catctcccct
      721 tctccccgta aacaggggac cacaaaggag aggtttcctg gcgtgtgttt ttacaaggtt
      781 aaaagtttat cataaggtac ctggtttttg caatacagcc tatctcattt tttgattgca
      841 tttcgcaagg aaaattgcta tatatatagc gtttgagagg gagggttatc tgatactcgc
      901 ttcaatcaca cgatgctccg gtatcaccgg tatcagggga atgaacgtat tgatctgcac
      961 attgcggaac cataaccgtg aaagagggga aaagatgcct gccctgcaat gggaacccat
     1021 cccagatatc aatgtataga aaccagacat gctgtacgga aatccgccaa agaaccggcc
     1081 aattatcatc aggatttgaa ttccttaagg actgcaaaaa cattaaatac ctgcggttta
     1141 ctgataaatt catggtgagg catagttttt gctttcaaat taatgctgaa tcaataaagc
     1201 atagagaaag ggattaatgg tgtgctgaaa gaaaaggacg tccggtattc tgtcaagcat
     1261 gccacggtta taatcttatc agatgccact caaggaggtg gagggtgact ctattgagaa
     1321 gcagtttttg tttcttgact gttcttgtat tggggctgac aggatgcgaa gccctgaagg
     1381 cttccaagcc catcattccc ataaaggaat acgaacagat gatcgcggga aggcttgatg
     1441 cgaattatgt gggtacgcgc acctgtcttt ctgcctgcca ctttcacgac aagatacggc
     1501 gtgattttga ggcaagcacc atgggcgccc agctatcgag caagtccggt atgcctctgg
     1561 ttgactgcga atcatgccat gggcctggga gcctcgctat tgaagggatt acccctgaga
     1621 aggtcgcaat ggacgcaaaa gagggaaagc agactgcgtg ctattacaag acccttatcg
     1681 agatcaagaa tcttcctgcc ggggcgcagt ccctcatttg ccttaaatgc catacggcga
     1741 atgccacgtt taacctccat agttggaatg cgagcgctca cgcgatcaac gacgtttcat
     1801 gctcagaatg ccacaaggtc catgtgggac ctgatttgac ggtgaggcga cgtgcaatgg
     1861 cggagatgtg tgacagatgc caccaggaag tcagggcagc gttttatctg ccgagccacc
     1921 atcccgttcc ggaacagaag gtcttctgta atgactgtca tgatccccat ggctcctttt
     1981 atacaaagtc catgaaaaaa ggaacgataa aggagacatg tagtcaatgt catatggaaa
     2041 aggctgggcc cttcgccttt ggacatgctg acaataccga agactgcacc acatgccaca
     2101 gtcctcatgg ctcggtaaac aactggctac tcaccctcag ggtgccattc ctctgcctcc
     2161 agtgccatcc tggacacagg acctccaaaa cctcctcagc agagtcaaag ggtgcgtttt
     2221 ataccaggtg cacagattgc cattccacga ttcatggaac ggaccttccc tctgccagcg
     2281 gcaggggaag gttcacccag taggggagga gggtgagatg aaaaaaatac tatcaatagc
     2341 tctcgcggtc ctggtctcga tgatattggt tcacatggca tattctcaag aaactgcagc
     2401 accacaggtt gctcaggaat caggcatgga agagacagcc ccttcagaag cttatacatt
     2461 ccctacaata aaacccgaag catcactttt cttgggcggc agattggcag gcttcagcgg
     2521 gtctccaagg gctgatcagt atgaatacct gcatgacttt gtcccgtttg ggggagagct
     2581 gaggttgttt gagtatccgc acagacttca cctcgatgta gatattcagg atcaaaaaga
     2641 ttatttcggg gatttgagct actcatataa agacctcatt ctcttcaggg gggttgacag
     2701 gactgtgtgg cacaacttgg tgaacatccc gctcatagat ctcaatcccg caactccttc
     2761 acctggagtc gatgtcagag acagtggggt agagtacggg acaaggacca gtatgaataa
     2821 tgcatccctt agatttaaga tgccggactt cccactccat atttacctag acggcagcta
     2881 cattgacaaa tcgggggggt ccatgcagca gcggtttctg ggaggttccg gttatttcaa
     2941 caatattgtg agggtttcgg agcagcgcga tgtggactgg gtcaccaaag ggctcctcat
     3001 gggtataaac agccatttag ggccgataga agcggacttc tctcatggcg aaagaaggtt
     3061 cgattcctcg ggcacgcagg tttttcagga tttctataca gatgcagggg tcagaccagc
     3121 gggagtcttc gagcacaacc tcatccctga tttcaagagc tcatcaaata ccctcaagct
     3181 ccatacttca tatacaggag gcatcgtcgc atccgcaaca ttatcaaata tagatacgaa
     3241 gaacgatagc agtgggacga aggcagacta ctacatcggc acagcggatt ttacgtggat
     3301 gcccatgcca aagctgacct tttacgtgaa gtaccgttac aaagataccg acttcgacaa
     3361 tccgggttca gttacgattg ccgacctctc caacccattt cagaccttta cctaccctgt
     3421 aaaggcggcg atctcatcca taacgaatac cgtctccgga actgtacggt atctcccgat
     3481 caaaggggtg acgctcaggg cagaatatgc gtttcagaac atcgatagag acaatgccag
     3541 tttgtggggt cttccggatt caacccagag caacactgtc acaatctcag ctgatacaag
     3601 aatcctcaag ggcctgaact tcaaggccca atatacctat ctgcagatta gcaacccagc
     3661 atataacacc gacccaaacc actctaattc agccaaattc tccctttcat ggattcccat
     3721 accccaagtg aacacctatg tgagttatac ctttgcaagg gagaagcggg ataatgtgtc
     3781 ttttgttgag gcggatggta ccatcacaac tacgcctaac aacagggacg taaataagga
     3841 tatggttatg gggagtgcca cgttcctcat attaaaagac ctgtctctca ccggaagcta
     3901 cgcctacatg cataacaaca cagagcagga tatggggtat agtgacctgg cagggattcc
     3961 acagactgac ccctctgtgc ctaacaaaca aacggcgcag acggttgcgg ccgatctgaa
     4021 ttatatgccc aaaaataata taaccctcca cgccggcttc agctatacat taagcagcgg
     4081 gcaattctac cccagcagtg cggacctcct ccagccagtg tctgtagcct ctttttcagc
     4141 cctcaaagag aaagagacag cgtacactct gtcaggagaa tacagattta agaatgggtt
     4201 tggattggag ttgcggtata agtactgtca tctgaacgat gtccagaata atccatatga
     4261 tgatgtgcag aacgggaatg cgaatatctt catggtactc ctgtcaaaga ggtggtaggc
     4321 atatgatgaa taaggttaag aaatcacgaa ttctggcgtg cgccttgaga gaacggaccc
     4381 cttttgcggt gttgctctat gtatggacta tggtggtgct tgcttcgatg tgtttctctc
     4441 ctcccacctt tgccgctgaa aagaccgtcg gggtgatcat gactggcaac atctcctact
     4501 ataaggacat tcacaaggca ttcatcgaag ggctgaacca ggagggtctc tttgcccagg
     4561 gaaaggtgga tgtcgtggtg caaacccctg cccctgagac catggcatgg acaaacgcgg
     4621 ccagaaagct tgtggcaatt ggctccgcag tgatcgtatc atacggtgcc cccgcaaccc
     4681 tgtcggttat gagtgagacg tctgatatcc ccattgtgtt cgcgggcgta tatgaccccc
     4741 aggcccttgg catcacaggc aagaacatta cgggaataag cgcaaaggtt cctgtgacga
     4801 gtctgataaa aaatctcaaa agcatctcga atttcaagac gctcggggtg attattaacg
     4861 actcggagaa agacagtgtg ctgcaggcaa acgaggtgaa gcaattggag ggcagttttg
     4921 ggttcagctc caaaagcttc aatgtgaaaa gggcggaaga cgcttctaga attacgggtg
     4981 ttgacgcgct ctatctcacc acgggatgca ccgggatgca ctgtgtgacc aatattgttg
     5041 gcgttgcgcg gaaggggaag atggcttcgg gagcaaccat aggaggagga gaggccagtg
     5101 gagtcatact caccatagcc gcaaatcccc aggagcaggg gagagaggct gcagagagag
     5161 tggcaaagct catcaagggg gcgagtgcag cgtcccttcc ggtcgagcag cctaaaaaag
     5221 tggacatgat catcaacctc aaagaggcca cggatatggg actcaaggtg cccttcgacc
     5281 tactcacctc ggcaacaaag gtaatcaaat gaatcgtggg ataaacctcc agaaaaaggc
     5341 aattattatg gtcggaggga tactcttctt tatccttgcg atcaacaccc tcgtgctcac
     5401 ctcggtcgcc tcaaataagt ataagagcgc attgctttcg aaaacaaccg ccgtgggtga
     5461 agggatgcag agagatctcg gcagggccct gaatctcgga atcccccttg agtcaatgga
     5521 aggggtgaat gaaaaactcc aggagcttat ttccagggat ccggcaattg ggtacaccat
     5581 ggtcacggat atcaagggga aggttttctt cgatacccgt caggcagagc tcggcaagga
     5641 actcaaagat gacgcatctc tgaaggcggc ctcttcaccc aagtccatta tccaaacaat
     5701 aggcgctttt tatgacctct ctttccccct gatgaatgcg gaggggaaga tggtcggtgt
     5761 ccttcgcata gggatcaaat cagaggcggt gaactcccag ctgtatgcac tccttctgtg
     5821 ggccctcggg atatcgttgg tgagtttcct tgtttccctt ggcctcgtat ctttttccat
     5881 ctcccgattc atcaccaagc ctattatgca catggagaag gctgccgaag ggattgccgc
     5941 aggcgatctt acctctgtca tcgagactaa agggagcgat gaaatcgcag ccctggggaa
     6001 ggcaataaac gggatggcat tcaaccttaa agacatgctt tcaaaaatca gaaacatctc
     6061 caatagcgtt acccatgtca cctcaaatat cgtgaattcg tcacaggggg tgttagccgt
     6121 tgctgatgtc cagaaaaagg cgatagaaga gaccgcagtt gccatgaccg aaatgaacag
     6181 ttccacgtcc agtgtggcgc aaagcgcgga gagcctttcc gattcgtcgg tggacacctc
     6241 atccgcaatc ctgcaaatga ccaagtccat tgaaagggta gcagagagct caaatatttt
     6301 cgacgaacag acccatgaag ctgcttcatc catagaagag atgatctcca atatcaaaca
     6361 gattacccag agtctggaga acctctcttc atcgtctgag gagattgcgt cctctgtgac
     6421 cgaggtggac gccacgatca aggaaatcga gcatcacgcg ggcgagtcgg tgagacttgc
     6481 ggagaaggtt acgaccgatg cgtcggataa gggaatgaat gcggcagagg ctgccataga
     6541 ggggatggag aacataagga agagcgtggg tgccctctcc gatatgatca acatactcgg
     6601 caaaaggtct gaggacatcg gtaaggtgct caacgtcatc gatgaggtcg ctgaccagac
     6661 aaacctcctt gctctcaatg ccgcgattct tgctgcccag gcaggcgaac acggcagggc
     6721 atttgcagta gtggccgatg aggtgaagag cctggcagaa aggacgtccc tctccacaaa
     6781 ggagatcgcc gcgctcatca cctcagtaca ggaagataca cgatccagcg ttcagatggc
     6841 tgggcaaggc atcgagacgg tggaaagagg tttgggactt gtgcgggatg tccacgaggc
     6901 cctcagcggg atactggaca gttcaagggt ttctacagaa atgtcaaagg caatacaacg
     6961 ggcaacttcc gaagagtcac aggtaataaa gcagattacc gactctatca aggggatgtc
     7021 gaagcaggtg gagaacatct cacgggcagc acaggaacag agcaaaggaa gcaaattcat
     7081 cgtcgaggca acagagaaga tgagggagat ttcgcatcat attaaggttg cgacagggga
     7141 gcagaaagac gggagcagac agatctccgg tgcagtggaa aatgtcacgc agcaggctga
     7201 tcatatcgcc aaggccacag gcaagcaatt agagaagagc gccgaaatcg tacagtcaat
     7261 ggataagatt caaaatacca cgggcaaatt gatacaggtc tcacatgaga tgagcgcagc
     7321 catcaattct ctcaaggaag aggcccggaa ccttctctca gaactccaaa aattcaacgt
     7381 gtgaattaat tcctgcagat aattgcccat cccttttgaa aaatagcctg ctgttcgggg
     7441 catacaccgt gaaaatacaa ccccctatta tagggtccaa aggaatagga agcagcaggg
     7501 cttgggacct ggagacgctg attaattccc tcagcgaggc gattctcctc ctcgacaagc
     7561 gcaacacaat cgtctttgtc aataaaacgg gtgaggaatt cttcgggaag agccatcagg
     7621 agattatcgg agcaaggctc accgacctct atccaggtgc gaagaccatt gcaaggctgg
     7681 caagaaaggc aatgaaagag ttgaggtcct tcagcgcgaa ggatgtggaa gcaaaaatca
     7741 atggcatttc cagtgtggat atcgatatta ctccgtacct cactgatgat aaatgcagcg
     7801 gtgtattgct ttctatccgg gagaacctct ctatcgcgga aagggaggac tcttccttcg
     7861 attcccttgt ctatctcctt ggcaccatag cccacgagat caagaatcca ctgggaggca
     7921 taaaaggggc tgctcagctc ctgaagggaa agctcctcaa gggtgaagcg cgggcctttg
     7981 cgggcgctga taccgtagat gagtatatca acctcatcat acgggagacg gacagattga
     8041 atacggtctt gcagaattac ctcacgatga gtcggaggcc cgcccttcag gagatgaata
     8101 tccatgaaat cctggaaaag gccatttcga taatggatgt tttgctgaaa aatgttaata
     8161 taggaatcca aagggcgtac gatccgagcc tccccagggt tatcggagac gggggcaagc
     8221 tgcttcaggt ttttctcaat gtcatcaaga atggcattga ggcaatgccg agggggggga
     8281 acctgagcat tgcgacaaag ccgtccgccg aatatgcggt gcagcacgga cagataaaac
     8341 gctgggcagt agtgtccatc agggacaccg gcaagggtat ccccaaacaa gagatgcaga
     8401 agatcttcct ccctttttat acgaagaaga agaagggtac cgggcttggt cttgcccttt
     8461 caaagaagat cattaaggat cacaacggat ttatacgggt ggagaggcct gaatcgggaa
     8521 agggaaccgt gttccatatc tatattccct ttgcccagtg aggaattgac tttttttggc
     8581 acacgaagac cacatgggaa aagaagtact catcatagat gacgatgaag ccatcacctg
     8641 ggtggtgaaa aaggcgcttg agcccctggg ctataaggta gtgacacgcc ctcgactctc
     8701 ttccgggctt aaggcggtaa agggcggcct tcagcttgtc ctcctcgaca ttgtccttcc
     8761 cgatggaaac ggcctcaatg cactcaagga gatccggtcc accttcccgg atgccatggt
     8821 catcatggtc accgcacacg gcaggatgga gagcaccgtg gaggcgatga gagagggcgc
     8881 ctatgactat atcgagaaac cctttgatgt ggaagaactc aagattaatg tggaaaaggc
     8941 attcagggac atggcagtga gagaggagct ctcaaatctc cggagtgtaa cggcggaggc
     9001 cgaggaaccc ctgatcgtgg gaagaagtcc caagatgctg aaggtattca aggaaatcgg
     9061 cagggtggca tcaaaagata tcaccatatt gatcgtggga gaaagcggga ccggcaaaga
     9121 gcttgtggca aaggcgattc accataacag caatcgcagg accggaccat ttgtcgccat
     9181 caatgcggcc tctatcccga aggacctcct ggaggctgag cttttcggat gggaaaaagg
     9241 ggcgttcaca ggcgcgaaag aaaaatatgc ggggaaggtc gaatcagccc gcggtggcat
     9301 cttgttcctg gacgagattt ctgaactgaa ccttgacctt caggcaaaac tcctgaggtt
     9361 tatgcaggac aagaagttca gccccctggg gagcaacacg gtcatggaag ggaatgtgag
     9421 aatcctcggc gcaacgaaca gggacctcaa ggaggccatt accaaaggat tgttcaggga
     9481 ggatctgtac tacaggttca atgtggtaca gataaaactc cccacgctgg ccgagaggcg
     9541 ggaagacatc atccctctcg cgaaacattt cctgaaagat gctgagagaa aatttgagac
     9601 agggaagaag gaactgtccc gggaggcgaa ggacttgctg ctcaaatatc cctggccagg
     9661 caatgtgaga gagctcgaga atgccatcaa aagggcatgt atcctttcca acgggacagt
     9721 catcgaaaaa cgcgaccttc ccttggaaga agccaattcc ttctctatcc aggactttct
     9781 cgaggagaaa ctgaagagat atctcaaaga gatgaccaag ctcgaaaagt gcaaccttta
     9841 tgattcggta gtctcggagg tggaaaaggc ccttatcaag attgtgctga acgaaaccgg
     9901 aggaaaccag cttaaggcct caaagaccct cggaatcaac agaaataccc tgaggacgaa
     9961 gatcagggga tacaagataa agtgattgaa ttttatgaga gcgctgtggt ataaataacc
    10021 gctccttgct ctcgctcttc ctctgcggag aggcgagggc tcttccgcgc tgaaggtcat
    10081 tgacctctgg cggatccccg agaggggaaa atccataagg aggtgttcag atgaacatct
    10141 atgaaaacat cgtaatcctc aatgcgtccc ctgccgatga agagatcgag gcgtccctca
    10201 acaaaataaa agacctcatc accaatgcgg gaggcgagat actcaaggtt gatgcatggg
    10261 gaagaagaaa actcgccaat gaaatcaaaa aacaaaagaa ggggttttat gtgctccttg
    10321 tcttcaggtc ggcctcatcc ctcgtcagga aactggagga ctattacaaa gtcttcgacc
    10381 ctgtgataaa atacatggtg attaaacttg ggagcaagga gagcacacat tttctggcaa
    10441 ctctgatcca aactccaagg ggtgacgagg caacggccaa gccggccccg ggtacagcga
    10501 cccctgcgcc gggtgcggct tctgagtaac ggtctgcggg gagaagagag ggcacatgta
    10561 taacaggata atactgatcg gcaatctcac caaggaccct gaactcaggt ataccccgca
    10621 gggcactccg gtggcttcct tcaggatcgc ggtaaatacc cggtataagc aatccgacga
    10681 atccagagag gataccctgt ttatcgatat tgtcaccttc gggaaacagg cggagacatg
    10741 cagccagtat ctcaataagg ggaagtcggt tctcgtggag ggaagactcc aggagaggag
    10801 atgggaatca gaaggccagc agaagagtaa attcgaggtc atcgcgcaga cagtgaggtt
    10861 tctgtcaaga aagggcgcgg gagagggtca gggagttccc gagagcgagg tccctcctcc
    10921 ggaaggcatg acagaccccg aaccattcta ggttcggtta ttactattaa gtactattaa
    10981 gccactcaca taggagaaga aaggagtcca cattgcagca gagaagattt caaaggagaa
    11041 agttctgcag gttctgtgca gagaagtttg aattcattga ttacaaggat atcaagacat
    11101 tgagagggta catcacggag cggggaaaaa tcctgcccag gaggatgacc ggggtatgtg
    11161 caaggcacca gaggagtctc aatgaggcaa tcaaaagggc aaggaatatc gcccttctgt
    11221 cattcgtgga aaaatagatg aggattccca ggacgtgggt tcctcttatt gcaaaaaggg
    11281 tcctggacga tcttatcgca aaggggctcg tcatttcgaa ggtgtccacc gaagtcctca
    11341 tggcagaagc ggaacgcctc attatggaag aactcattat cgaggacagg atcaatgacg
    11401 aggtgagaga gatcctcaag aagtataccg gggagatgca gaaggggagg cttgactaca
    11461 gaaagatgtt cgagctcacc aagaagaaga tcgtccagga aaggggcgtt atcctctgat
    11521 gctctcggat gagaaagtct cccatctcac ccatcttctt tttaagggat tgaccgacaa
    11581 agcgctaata gaacttgttg cagaagaagg caaggtacga cgagaggtaa agcggtcaat
    11641 tgtgtccttc ctcaaggtga gcgaggagat cgacctgtct gtaaggaaga aactgcagtc
    11701 cttttcgaga aaaatcgttg aaggaagtcc cgaatgggag gtgctctaca aaaagttcta
    11761 cagggaagag gcggcaaaaa gaggtttggc atcggagtaa gaagggcgct tttgccgttt
    11821 ctccttctcc tgtcgtattc agggcttatt ttcaatttcc cttcaacgag gccctcagtc
    11881 tggttttgct tcagcctttt ccccgttgcc gcatatgtgt tcctctgctt catacaacgt
    11941 cagaaaaaat ataccttcga gaccctggct tcccttgtcc ttctcatggc aggagggata
    12001 cagttcctca gacatgactg gcttcatggg ctctacgtcc cctgtcttgt cattttcgct
    12061 tttttctacg gaccaagggt ccttatccct ctaagcatta ccatcccctt tctcgagatc
    12121 agacattttg tgcagggcaa tcccatggag gaagcctttt ccagcggggc cgctgccctg
    12181 agcggcatac tcgtgtctct ggttgtctca aggacgaggg aagaacgtga caggcttaag
    12241 aagtccttcg atgcgctcaa ggaagaggcg gagaatatgg atcctgtcac cactgcaggc
    12301 attacgagca ctaatggcct tgcctctcag catctctcct cggcgcgcag gacaaacgaa
    12361 gagatacggg aagtcctcct aatagccaaa cacgcgctca ctgcagattc tgcgcagatg
    12421 ttttcccttg aagaaaaatc cctgcacccg cggtgctccg cagatgagtc gtcatccgtt
    12481 gatatccctg actcgggccc cctgctgtta tgcctccaga aaaggcagtc ccttgtgttt
    12541 actagcggcg cgcacaagaa taccccgctt ctcagcaata ttgcagtacc gattgtagac
    12601 gggagttttg catacggcgt gctcactctg cagggaagga aaggggtgct caaggagaac
    12661 gatccgaaga tggtggagat gttttcccgt cagatcgtga atattctcaa cagacagcgc
    12721 atctattccc agttgcaaaa ggaacacctg atgctgagaa aactgaagga cggggggaca
    12781 aggatgatct catcgctgaa gatggatgat atcgccccca gtcttattga tgcagtatac
    12841 agtatcgcgc cccaggagcg ggtctccata gccctgtttg ttccgaagga ggagcaattt
    12901 gaaatggtcc ggcagatcgg atttgcgctt tcagaaggaa gtacctttga tctcggctct
    12961 acgcgtatcg gtctggtggg aaggagcagg gaacctgatt atatctctga tttaagaaac
    13021 gagcggagcc aggcgctgcc gttcaagata cccgatgagg gctccctctt catgatgcct
    13081 ctctcttatg aaaaggaact cctcggtatc ctcgtattcc tgtcccccat ccctaatgca
    13141 atccgcccct accaaatcga actcttaaag gtgctcggga accaggcggc ggcgtccctt
    13201 gcaaacgcga agttccacgc agagatagag agaatggcca ttaccgacgg tctcaccgga
    13261 ctctttaacc atagaaattt tcaggaaaaa cttactgccg agttcaggag gctggagcga
    13321 ttctcagcct ccctttccct cctcctcatc gatattgatt tctttaagaa aatcaatgat
    13381 agttacggac atcccgcagg tgatgaagta ttgcgggagg tttccaggat catcagggaa
    13441 accataagga gtgtggatat ccctgcgcgg tacgggggag aggagttcgc ggcactcctg
    13501 cccgggacaa accatgaagg tgcgcttaaa atggccgaga ggctgagaga gtccattgag
    13561 aaaacaaatt tccccattga aggaaaaaag ttaagggtga gcgtaagcat tggtgcagca
    13621 acctctcccc atgatgcagc agcaaaagag gaacttgtgg aaaaggcgga taaggcgctt
    13681 tattatgcca agaggaacgg gaggaaccga tgtgtgctct ggagggaagt gaagtaaatg
    13741 gaacctttca tttcctacgt aacggaagag gagatcagga gggcccgggc ccgggcccgg
    13801 gaattaagaa agactcagtg gtggaaaagg aaatgtgctg ccggaatatg ccattattgt
    13861 aaaaagaagg tcaacccaag ggagctgacc atggaccata tcgtccccgt catcaggggg
    13921 gggaaatcca caaagggaaa cctggcccct gtgtgcaagg agtgcaatga caagaagaaa
    13981 tatctgctgc ccatcgaatg ggaagagtat ctggaacgct tgggcaaaga ggaaatctga
    14041 tatgcgtcat agtattacac tccgcgtggg ggattgaggg gaaaacagtg gacatattcg
    14101 caaagattgc agaggagaga ataagacagg caatggagga cggggaattc gcaagcctgc
    14161 cgggttcggg aaaaccgctc atgcttgaag acgagacatg gatacccgag gacatgcgac
    14221 tctcctacag ggttttgaag aatgcgggct gcattccccc tgagcttgaa aagaggaaag
    14281 agattctgaa ccttaaggaa ctgctgaaca ctattgacga cgataaagaa cgactgagga
    14341 aactgaggga actcaatttc aaactgatgg aattcaatat cataaagggg aggccccttt
    14401 atcttgaaac ctttcctgac tatgagcaac gtattttcga aaaaattacg aattcgtgac
    14461 cacaggctgt ctcacgtcaa aagtcctatg tgataaatgg cgaccatggg tgaggataca
    14521 cggagtacaa tagtccttgt ggtctgctgc actctcatta ttgcggctgc cgcggccttg
    14581 ggctggagtg gcgatgatat tcttggtcta tggaacaacg aggaacggga cgggataatc
    14641 gaaatcttca aatgcaggga ggactactgc ggcagaattg tctgggcaaa ggaaccaacc
    14701 taccctgccg acgacaagat ggggagggca ggacagccca ggctggacga caataatccc
    14761 agtcctggcc tcagaaccag gcctattatc ggtctccaga tcatgaacga ttttgttttt
    14821 gacggagaca acggatggaa agaaggcaca gtctacgatc ccaagaatgg aaggacctat
    14881 agcggcaagg tgacccttgt ttccccgaat gagctgcggc tgagggggta tgtacttttc
    14941 tcattctttg gccgcaccac tatctggaca cggctggagc attgatccaa ctggtcacga
    15001 cttccgtaag agataggcat gtgcggagag gcctgcgcca aaggcgacca taacgcagaa
    15061 atctcccctt tcaattccct tttccaagag atcgttcaac acgaaccaaa ccgtgggcga
    15121 tgacatgttc ccatattcag caagaatggc acgtgttgaa cgcaattgtt cctcggaaag
    15181 tccgatttcg gccttcacag tgtttattat cttttctccc cctgtatgga gtgcccagtg
    15241 ctttatctct ccgacccgca atgcccttgg ttcaaggaca tccttaacta cctgtgcgac
    15301 cgccttttta acgaggtgag ggagccttgt tgagagttga ttatacagct gaccgttctt
    15361 gtggatatag cgtattgcgt cacggtcttc gggaacataa tagctggatg atgcgacaag
    15421 ttcgatcccc tgagggcggc cccagagcac tgcagcagcc gctccgtcgc tgaagagggc
    15481 attagagacg agcagactca ggtcgtcctc catctgaaaa gtagcggtgc atatctccac
    15541 cgaaacgctc acaatcacct tgtgtgaggc acccctcaca agggattcgg ccacctccaa
    15601 attcggaatg gctccgccgc agccgctgcc gacaaggtca tataccctta tatgacggtc
    15661 aagccccagt ttttcgataa ggtacgtcga aatgccggga cagatataac cggtgcatgt
    15721 attcactaca agccctgcca tgtcgtcaaa ggacaggcct gccttaacca gggcgttccg
    15781 tattgcctga gccgaaaggt ctactgccca gcgcgtgaat cgctgaatgc ggtcatccgg
    15841 attctcgcta aagaaacctt cagagtcgtc aacggcaaat ctcctcttgc ggatggccgg
    15901 atgagcgaaa agacccttca taagcgtgag gctcctcgaa ctcaacctgt ctgaataccg
    15961 gtgagcaaga attttcccca cctcctcctg ctgaacggtg aaaagcggtg tctccactgc
    16021 aatggatccg acatacacat ttcctgacat ctgttctttg ttacgcacgt tatacctcca
    16081 ttcctaatag cctgagcccc ctcctgatga aactggtatg cttccacaag ggagaacgaa
    16141 gtcccacctt atacgcgagg cggtgaatgg acggaagaag gggtgaactc agtgagctgg
    16201 aattcatgaa attcatgtgt tcgttcattg aggtttctat ttgtttccgc atccagttgt
    16261 attcaacggt gggcgagaga taaaagaccg gctcaagcat ctgagcggac gagtgagaga
    16321 ggagcccctg tttccttgca atggcttcca gtccggtacc gggatagaca cgtatgccca
    16381 tgccaagaaa cacagtatct gtcgggcgga tacacctctc tgcaaaccgt agggtctccc
    16441 tcaccgtccc ctctgtctca ttgggccctc ccatcatgaa tatccagaca caggggagcg
    16501 tatgacgccg aacgacctct gcggccttat gcacatgctc ttttgtgtat cccttcccaa
    16561 gcccctcaag gacgctatct gatgcgcttt ccacggttat tccgataccc gagaaccctg
    16621 cacgttccat cgcagcgatg agggtgtcat caatggaaag aggattcagt tccaggctct
    16681 caagagatac gcgtgaacgg gcccgggcga ctttttcgca aatggaaagc gcatgatcat
    16741 acggggagtt gaacacattg tcgacaaact caatatgggt gaggccacct gcagtgagct
    16801 tgccaatggt atccacgacc tcttcgggat cactcaggcg gtatctgctc ccctcaattt
    16861 tccggtacgt gcaatagacg catgagaact gacaaccaag ctttgtctgg attggaacgg
    16921 tggagagtct cctgaggtat gtcttcaggt caatccagcg ccggaagtca ggtgaggcgc
    16981 atgaacaacc attcattgat gaagcgcatg gattgacatt gaacgtaccc ttctcgatcc
    17041 atgcaattcc cggaagcgtt tcggggggtt cttcctgaga cagtttttca agaagcatcg
    17101 ggaacatggt ttctccatcc ccgagtactg cccaggaggc accagtataa cggagcagct
    17161 cttccggcat aacagaaacc gccgcccccc ccagcgctac gggcgctgat gtcatgcctc
    17221 gcacagaatc gatcaggggg aggatatctt ttagaaaaaa gttcggagac tggatgtcgt
    17281 tattatcgat gttccttacc gaaagcccta ctatatccgg gtttcttttt tccagttcac
    17341 gcttcaccac ccgcagaggg tcttgctgaa acatcagatc caggagccga acctcgtgtc
    17401 ctgcacgcgc tgccgcatct gcaaccaggc atgcacctaa gggcatgacc ggcatgggga
    17461 actggttgct gtttgtactt ataataagga ccttcattct cttcctataa taccattgcg
    17521 cacgatgatt ctgaattact acgaaaggta acatcagcca gggtgttcct catcactttc
    17581 ttatatattc cttttaggtc gataataatt ggtaattctt gtttcttgcg gtaaaatgaa
    17641 aagaaaagtg atcgccgaac attttgaggt cctcttgata aggagaaagg agaatatcca
    17701 tggacaggaa gtcaaaaggc ttaatcgcag tgttcgtaac agtccttctg gcgtttgcgg
    17761 catgtgcaag ccaacagcct ctggtcacta taggcccggc aagaggcgaa attgtgctcg
    17821 caatgaaggc cagcgatttc acgtttgagc caaacaacat taaggcttat aaagatgatg
    17881 tggttgtatt gaagattgaa aacatttcca gtacaggaca caattttacg ctaaaaacac
    17941 ctctggggaa cgcggagcag agcgtgaagc ttcctccaat ggaaacagtg acagtcaagg
    18001 tcgagctttc tgaagcaggc acgtatgagt tttattgcga taaaccgttt cattcaacac
    18061 tcgggatgaa aggccagatt gtggtcaccc aaggaccata agcaaggtca tatagagcaa
    18121 tgcgtgcaga aaccgaactc acgacgggtc tccaccaata atctttcccg aatatgtgtg
    18181 gacaggcgtc tatcgtgact atataataag tcgaggatac caggaaaccg ccaacgatat
    18241 cttcagtata taaaaaggcg caattgtcta aaggaggaag aaaaatgaga agaaagggtc
    18301 agggcttatc atttatcggt gcattcgtga ccgtattgct ttttattttc ggagtgttct
    18361 tcactgcagc aacccctgta atggcagcgg acgtaaacga ggcgcagggg tttgtggaca
    18421 aggcgcgtgt caccttcaat gagttcatgc gcgacagcaa ctatggatgg ttgcacgaac
    18481 atctcaaaga ggctaaaggc ctcctgatct ttcctcaggt gataaagggc ggctttttcc
    18541 tcggtggttc tggcgggacc ggtgttcttg tggcgaggga tgcaaagaca ggggactgga
    18601 gccagcctgc cttttacacc atcggctcgg tgaccttcgg tctccagatc ggtggtgaag
    18661 cagcagaggt gatcatgatg gcaatgagcc agaaggccat tgattccctt ttcgcgtcat
    18721 cattcaagct cgggggagat acctccatca cactcggccc tgttggtgct ggtgccaagg
    18781 gcaatatcac cgctgatttt atttcctttg caaagtcaaa gggcctttat gccgggttga
    18841 atcttgaggg ttctgtagtg gacgtaaggg acagcctcaa cagagcgtat tatggtaaag
    18901 aggtcaggcc ggtagagatt gtagttgaga aaaaggtaag taacaaggga tcttcagaac
    18961 ttcgcacaac cctgaaaaag gcggtcaaat agacctgttc gcgttttcat tgagacgggg
    19021 aaaaggatct cctgatgttg agactgttaa ccggagagaa acaaaaaaag agacaacact
    19081 gttggagtca ggacctatgg ggcgtggttc tgcccatggt cacaggaata acaaaggagg
    19141 tttcatgaag ttaggaagag tctttaccca tgcagtgctt tggttgctgg tagcacttat
    19201 ggccgcgccg ccatgggctt ttgcgcagga caccgaggga gggccttctg cggcgttttc
    19261 cagggaacag cttgaacaga tactggcgcc catagcgctc taccctgatg aattaatcgc
    19321 gcagattctg atggcatcga cctaccccct cgaggttgtg caggccgacc gctgggtcag
    19381 gcaaaacaac aacctcaagg gcgatgccct tgccgatgcc cttgaagaac aagactggga
    19441 cccaagcgtt aagtccctgg agaactttcc ccaggtgctt cagatgatga gcgagaagct
    19501 tgactggacc cagaagcttg gagatgcctt cctcgcacag gagaaggacg ttatggacac
    19561 tgtccaggtc ctccggagaa aggccgaagc tgcaggcaac ctgaatacca cacaggagca
    19621 aaaggtgatc gtggagaaag agacgatcat catcgaacca gccaatcccc aggttgtcta
    19681 tgtgcccacg tataacccca ccgtagtgta cggtg
//
LOCUS       DATKOU010000035         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_434621, whole genome shotgun sequence.
ACCESSION   DATKOU010000035 DATKOU010000000
VERSION     DATKOU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434621"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VMU21_01435"
     CDS             <1..472
                     /locus_tag="VMU21_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUO76214.1"
                     /translation="RDGTLCEDPHYLGSWDRFKLFQGVDELVRLKSKGFQLIGVTNQS
                     GIARGLVGEDFVKEVNRLFVERYGFDDFFYCPHLPEEHCSCRKPEPGMLHAARSRHGI
                     DLRKSFVIGDKEVDMLLAKTVGAKSILVRTGRDKESPYADFIAENLREAIGLVG"
     gene            478..1494
                     /locus_tag="VMU21_01440"
     CDS             478..1494
                     /locus_tag="VMU21_01440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO76215.1"
                     /translation="MPDKHKKILIIKPSSLGDVVHSLAVLDALYRCFPGAEIHWLVAK
                     GFEGLLEDHPMLKKLWIIHKDEWKKISRIRTTVAELRTLFRNLRKERYDLVIDLQGLF
                     RSGLIAKATGAPMRIGFKEAREGSTTFYTNMVEGGKDIHAVDRYLKIPAFLGCNVARV
                     RFPFPPSVQSLSPALPVPTEYAVMVPGARKPVNRWPARRFGELASELPMRTLVLGSRG
                     DRMLAEEVVRASQGKAESIAGKTDIRGLIQLIKGARFMVSNDTGPMHIAAAFGIPVFA
                     LFGPANPMRTGPYGKGHTIIRKDISCAPCYRKTCKNPRCLDMITTGEVAEIISNFLRR
                     NNDT"
     gene            1491..1940
                     /locus_tag="VMU21_01445"
     CDS             1491..1940
                     /locus_tag="VMU21_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUO76216.1"
                     /translation="MKKIYAFFVIWMVLFSANAFGSSAIHWFSLKEGTEKSKAEKKPM
                     IVDFFYGKGCPRCEALQKGVYDNPTIAKKINDEFVPIRVDLTKKLTEEEEKLGNKYDF
                     KNDCLLLFMDYQGNVMKDPSGKRLCFADTVEPEWFVQYLDMVKKQQK"
     gene            2183..2485
                     /locus_tag="VMU21_01450"
     CDS             2183..2485
                     /locus_tag="VMU21_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76217.1"
                     /translation="MKLGILVNTNRHLKDVVGLTKAAIAKGHEVMLFNMDDGTKLLGT
                     PEFKELCGTKGVIMSFCDHSAKHIGITTEGLPQEIVCGSQYNNAVMMHDAERVIVL"
     gene            2535..2705
                     /locus_tag="VMU21_01455"
     CDS             2535..2705
                     /locus_tag="VMU21_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76218.1"
                     /translation="MKILHILNDGPSPLSTKITGAQSRDHEVKIVDLSRKEMSYEAII
                     DAIVSSDKVISW"
     gene            2785..>3254
                     /locus_tag="VMU21_01460"
     CDS             2785..>3254
                     /locus_tag="VMU21_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUO76219.1"
                     /translation="MRKAGTILIGMVLAIALVVPVSAADDVAKKLNEVLMKGPAEGHW
                     QVKADDVDAWIKAKKTDFLVVDVRPNPPGQQGGRIPGSIYIPYNEILKPENLKKLPKD
                     KKLILVCVTGQTQNLPVVALRVLGYDARTMSFGHAAWIKDYLGAQLMQQAIQNAA"
BASE COUNT          870 a          734 c          883 g          767 t
ORIGIN      
        1 cagggacggg acactctgtg aggatcccca ttatctaggc agctgggacc ggttcaaact
       61 ttttcagggt gttgatgaac tggtcaggct aaagtccaag ggatttcaac tcataggggt
      121 gacaaaccaa tcgggaatcg cacggggact ggttggagaa gactttgtga aagaggtaaa
      181 ccgtcttttt gtcgagagat acgggtttga cgactttttc tattgtcctc atctgcctga
      241 ggaacactgc tcctgcagaa agcctgaacc ggggatgctc catgctgccc gttcccgaca
      301 tggtatcgac ctgaggaaat cctttgtgat aggagacaag gaggtcgata tgcttctcgc
      361 caaaacagtg ggagccaaga gcattcttgt gaggaccggc cgggacaaag aatcaccgta
      421 cgcggatttt attgcagaaa atctcaggga agcgatagga cttgtgggat agccctcatg
      481 cctgataaac acaagaagat actcatcatc aaacccagtt ccctggggga tgttgtccat
      541 agccttgctg ttctggatgc cctctacagg tgtttccctg gggcagagat acactggctc
      601 gttgcaaagg gctttgaagg tctcctggag gaccacccca tgctcaaaaa gctgtggatt
      661 atccacaaag acgaatggaa aaagatatcc aggattcgga ctacggtcgc tgagttacgc
      721 acactcttca ggaatctccg gaaagaaaga tatgatcttg tcattgatct tcagggcctt
      781 ttccggagcg gacttattgc aaaggccaca ggggcaccga tgcgtatcgg atttaaggag
      841 gcgagggaag ggagcacaac cttctatacc aatatggttg aagggggaaa ggatattcac
      901 gcagtggaca ggtacctcaa gattcctgcc ttccttgggt gtaacgtggc gcgggtgcgc
      961 tttccttttc caccctctgt ccaatcacta tccccggcac tccctgtccc taccgagtat
     1021 gcggtcatgg tccctggcgc gagaaaaccg gtgaacagat ggcctgcccg gaggtttgga
     1081 gagctggcgt cggagttacc catgaggacc ctagtgttgg gaagccgcgg cgacagaatg
     1141 ctcgcagaag aggtggtgag ggcatcacag ggcaaagcag agtccattgc cgggaagact
     1201 gatataaggg gattgattca gctaatcaaa ggtgccaggt ttatggtgag caatgacaca
     1261 ggtcccatgc acattgctgc ggccttcggc atccctgtct ttgccctctt cggacctgca
     1321 aatccgatga ggacagggcc ctacgggaag ggccatacca taattcgtaa ggacatttcc
     1381 tgtgctccct gttacaggaa gacttgcaaa aacccccgat gtctcgatat gataaccaca
     1441 ggggaggttg ctgaaattat aagcaatttt ctgaggagga ataacgacac atgaagaaaa
     1501 tctatgcttt ttttgtcatc tggatggtgc tcttttctgc caacgcattt ggatcttccg
     1561 caatccattg gttctccctc aaggaaggca cagagaagtc aaaggccgag aaaaaaccca
     1621 tgatcgtgga tttcttttac gggaagggat gtcccaggtg cgaggcgctt caaaaggggg
     1681 tatacgataa tcccactatc gccaaaaaga tcaatgatga attcgttccc ataagggttg
     1741 atcttacgaa aaagcttact gaggaagagg aaaaactggg caacaaatac gactttaaga
     1801 acgactgcct gctcctcttt atggattatc aaggcaatgt gatgaaagac ccatcgggca
     1861 agaggctctg ttttgccgac acggtcgagc ctgaatggtt tgttcagtat ctggatatgg
     1921 tgaagaagca gcaaaaataa cttctttgtg cttgtcactg ccttgctaaa acttgtattt
     1981 cagcttaatt ctctataaat atttcttatc aggagggcca gccttttgcc tttactttga
     2041 gagacacggt gtggtataaa ttgcaaaagt ctgctcttgt cgatgacgga tcgtccttga
     2101 aacggatagt gagagcattt cccgtcccga tgtatcggga ctgcggggtt cttcataaac
     2161 cgaacgcaca ggaggtaatc atatgaagct cggtatcctt gtgaatacca acaggcatct
     2221 taaggatgtg gttgggttaa caaaggcagc cattgcgaag ggacatgagg tgatgctctt
     2281 caatatggat gatggcacaa aacttcttgg cacccctgaa ttcaaggagt tatgcggcac
     2341 aaagggtgtt atcatgagtt tttgtgatca cagcgcaaaa cacatcggta tcaccacaga
     2401 gggccttccc caggagatcg tctgcggcag ccagtataac aatgcggtga tgatgcacga
     2461 tgcggaaagg gtgattgtcc tctgagcacc ctgcacgacg caatggatca tataccaaca
     2521 aagggggaat gaacatgaag attctgcata tattgaacga cgggccgagc ccattatcca
     2581 ccaagatcac aggtgctcag tccagggacc atgaggtcaa gattgttgat ctctccagga
     2641 aagagatgtc atatgaggcg atcatcgatg caatcgtttc gagcgacaag gtgatctcct
     2701 ggtaagggag gtggctgagg gacaggtgtc tttcggccgc agggttgtaa tgggcaataa
     2761 catttaccat aaggaggggg cgagatgaga aaggctggaa caattcttat cggaatggtt
     2821 ctcgccatag cactggtggt ccctgtctct gcagcggacg atgtggcaaa gaaattaaac
     2881 gaagtgctga tgaagggtcc tgcagaaggc cactggcaag tgaaggctga tgatgtagac
     2941 gcatggataa aggcgaaaaa gactgatttt ctggtagtgg atgtgagacc caacccaccg
     3001 ggtcagcagg gcggccgtat ccccggatcc atttatatcc cctataacga aatactgaaa
     3061 cccgaaaacc tcaagaagct cccgaaggac aaaaagttaa tccttgtgtg cgtcacagga
     3121 cagacacaga accttcctgt tgtggccttg agggtgctcg gctatgacgc ccgcaccatg
     3181 tcgttcggcc atgctgcatg gatcaaagat tatcttggtg cccagttaat gcagcaggcc
     3241 atacagaatg ctgc
//
LOCUS       DATKOU010000036         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1202948, whole genome shotgun sequence.
ACCESSION   DATKOU010000036 DATKOU010000000
VERSION     DATKOU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202948"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VMU21_01465"
     CDS             complement(<1..670)
                     /locus_tag="VMU21_01465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76220.1"
                     /translation="MFTVLHTESSKGWGGQENRILKESVGLGKYGIRVIILCQPGSTI
                     EKNACSEGIEVRTCSMRRHYDVSAITYILRLIRKEAIDVINTHSGRDSFLAGIAGRIS
                     RRKPVIVRTRHLAIPMTSRITYSLLPHVVVTVSEYVRRYLIREGVCPENVVTIHTGID
                     MTKFSPEAVPGNLRQEMGMAEETPLIGTVAILRRKKGYHVLLDAIPLVLKKFPHALFI
                     FVGDG"
     gene            complement(673..1824)
                     /gene="rfaQ"
                     /locus_tag="VMU21_01470"
     CDS             complement(673..1824)
                     /gene="rfaQ"
                     /locus_tag="VMU21_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HUO76221.1"
                     /translation="MIKLRHIGDVLLAVPVFRALREHFGDAHIAALVNSGTEDVLRGN
                     PLVNEILLFERKVKGLSPVKRYLREIQFLEEIRTRGFDMTIDLTGGDRAAILSFVSGA
                     RYRLGWRPGKGFPGKRRLYTHLSRPDTGKHMVLQNLDVVSQFGIDTGSLSVDLHIPEN
                     DRALVRAMLLEHKIPEGDTVVHIHPTSRWLFKCWKDEYMAEVIGWLVTQGLRVVITSS
                     RDPKEINKAKRILSLVSDVTPSLIPTPAGDGAGGVVDLCGKTTIKQLAAVSEMSSLFF
                     GVDSAPMHIAAAVGTPVVALFGPSGAFNWGPWDNAMALSGVQSATFKSPYSQRSGIQT
                     FGIHTVIQREWGCIPCGSDGCEGTKVSKCLDGISPGEVMDMLREKLEKH"
     gene            complement(1858..3000)
                     /locus_tag="VMU21_01475"
     CDS             complement(1858..3000)
                     /locus_tag="VMU21_01475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUO76222.1"
                     /translation="MKLAFIKKRFSVHGGAERYLQTLIRHLRKEGDEIHIFAHQWSEG
                     EGAIFHKIGIVPFGSFLSVVTFNMNARSALEKDHGMDCVVSFERTTCQDIYRAGEGCH
                     REWLTIRSGVEPFYKRLSFRINPLHLSLLALEKRLFSDTGLIVANSRMVKEQIMRHYA
                     VPEARITIIYNGVDLMRFTPENRGMWREKVRQDLSVSERGHLLLFVGSGFKRKGLKTL
                     ISAMALIKEEDIKALVIGGDEVSSYKRWATQRGIGERIVFLGPQREIEQFYAGADLFA
                     LPTLYDPFSNATLEAMASGLPVITTRNNGAAELIENGREGFVIPDPLDVQELADTLRR
                     SLPRLSVMAERAREKAEQYPIQRAAREFTEMIKRVKKADRCPDHHS"
     gene            complement(3142..>3492)
                     /locus_tag="VMU21_01480"
     CDS             complement(3142..>3492)
                     /locus_tag="VMU21_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO76223.1"
                     /translation="MEYREVHEKVVVKGSVGYLKEPIEHYSYRAIADYLRKMEQYSSL
                     SANELFQTKSTPLFLSMMANPVFVFIKMFFLRQGFRDGMHGFILAGLYSVYTFLKYVK
                     IWEKRRGDKGHEVS"
BASE COUNT          843 a          987 c          751 g          911 t
ORIGIN      
        1 ggccatcccc cacaaagata aagagggcat gaggaaactt tttcaggact aacgggatcg
       61 catcaagaag gacgtgatat ccctttttcc tcctcaagat tgcaactgtc cctatgagtg
      121 gtgtttcctc tgccattccc atctcttgtc gcaggttccc gggaaccgcc tcaggactga
      181 acttggtcat atctattcct gtgtgtattg ttaccacatt ttcaggacaa accccctcac
      241 gtatcaagta tcgtctcaca tattcgctga cagtcaccac gacatggggg aggagactat
      301 aggtaatcct tgatgtcatg ggtattgcaa gatgccttgt tcttacgatc acgggttttc
      361 ttctcgaaat ccttcctgct atacccgcaa gaaaactatc tcttccgcta tgcgtattga
      421 ttacatcaat tgcttctttt ctgatcaatc ggagaatata ggtgatggct gaaacgtcat
      481 aatgccttct catactgcac gttctcacct caatgccttc tgagcaggca tttttctcga
      541 tggtgctccc cggctgacac agtatgataa cccttatgcc gtacttcccg agacctacgg
      601 attccttcag tatcctgttc tcctgtcctc cccagccttt tgatgactct gtatgcagta
      661 ccgtaaacat tctcagtgct tctccagttt ttctctgagc atatccataa cctcacctgg
      721 agatatgccg tcaaggcact tgcttacttt tgttccctca cacccgtcgc tgccgcaggg
      781 gatgcacccc cactcacgct ggatcacggt gtgaatacca aaggtctgta tcccgcttct
      841 ctggctgtaa ggactcttga aagtcgcact ctgaacaccg gacagggcca ttgcattatc
      901 ccacggcccc cagttgaagg ccccgctcgg tccaaaaagg gcaacgaccg gggtgcctac
      961 cgcagcggca atgtgcatgg gcgcagaatc cacaccaaaa aaaagagatg acatttctga
     1021 cactgcagct aattgcttta tggttgtttt cccgcaaagg tctaccaccc ctcctgcgcc
     1081 gtcccctgcc ggcgtgggga tgagagaggg ggtaacatca gaaaccaaag acaagattct
     1141 ctttgccttg tttatttcct tcgggtcacg cgatgaggtt atgaccactc ttaatccctg
     1201 agtcacaagc cagcctatga cctcagccat gtattcatcc ttccagcatt taaagagcca
     1261 gcgggaggta ggatgtatat gcacaacggt atcaccttct ggtatcttgt gctccaggag
     1321 cattgccctc acaagggccc tgtcattttc cgggatatgc aaatccacag aaagactccc
     1381 tgtatctatc ccgaactgac tgactacgtc aaggttctgt aacaccatat gcttccccgt
     1441 gtcaggccgg gaaaggtggg tataaagcct tcttttcccg ggaaaccctt tccccggcct
     1501 ccatccaaga cggtatctcg cccctgatac aaaggaaagg attgctgccc tgtcaccgcc
     1561 cgtgaggtct atagtcatat caaaacccct tgtcctgatc tcttccagga actgtatctc
     1621 cctcagatac cgtttcaccg gtgacaaccc ctttactttc ctttcaaaga ggagtatctc
     1681 attgacaagg ggattacccc taaggacatc ctctgtaccg gaattcacga gggcggcgat
     1741 atgtgcatca ccgaaatgct ccctcagcgc cctgaagaca gggaccgcga gaaggacatc
     1801 gccgatatgg cgaagcttta tcacgagaat ctttctgaca ccctggaaat tcatcgttca
     1861 agaatggtga tccggacacc tatcagcttt ctttaccctc tttatcatct cggtaaactc
     1921 ccgtgctgcc ctctggatgg gatattgttc cgccttctcc ctcgcccgtt ccgccatgac
     1981 agaaagacgg ggaagggaac ggcgtagagt atcagccagt tcctgtacgt cgagagggtc
     2041 agggataaca aaaccctccc ttccgttttc gatgagttca gccgcaccgt tattccttgt
     2101 ggtgatgaca ggaagtccgg atgccatcgc ttccaacgtg gcattgctga aaggatcata
     2161 gagggtgggg agggcaaaga ggtccgcgcc tgcatagaat tgttctatct ccctttgggg
     2221 gccaaggaag acgattctct cgccgatacc gcgttgtgtt gcccaccttt tatagctgct
     2281 cacctcatcc cccccaatca ccagcgcttt gatatcctct tccttaatca gggccatagc
     2341 actgataaga gtcttcaacc cctttctctt aaagcctgat cccacaaaaa gcaggagatg
     2401 cccacgctct gataccgaaa gatcctgccg caccttctca cgccacattc cccggttttc
     2461 gggggtgaac ctcatcagat caacgccgtt atagataatc gttatccttg cctccgggac
     2521 cgcgtagtgg cgcataatct gctccttcac cattcttgaa ttcgcaacaa tgagccctgt
     2581 atccgaaaag agtctttttt caagagcgag gagcgaaaga tggagagggt ttattctgaa
     2641 cgagagcctt ttataaaaag gctccacccc cgaccttatg gtgagccatt ctctgtggca
     2701 tccctctccg gcccggtaga tatcctgaca ggttgtcctt tcaaagctca ccacacagtc
     2761 catcccgtga tctttttcca gcgcactcct tgcattcata ttgaacgtga ctaccgagag
     2821 aaaggaaccg aaaggcacga tccctatttt atggaagatt gccccctctc cttcagacca
     2881 ttggtgcgcg aatatgtgga tctcatctcc ctcctttctc aaatgcctta tgagggtctg
     2941 gagatacctc tcggcccctc catgaacaga gaaacgcttc ttaataaagg cgagtttcat
     3001 ataaaacccg ggacagacaa aggacaggga ggtgaaagat gttgatacga caccttataa
     3061 ggaaacccct ttgaagtaca cgtacatttc catcataaag aattgagcaa cgtcttcctg
     3121 ctctcttgtc gattgcaaaa atcatgagac ctcatgccct ttgtcacccc tcctcttttc
     3181 ccatatcttc acgtatttta ggaacgtata gacgctgtat aaccctgcaa ggatgaatcc
     3241 gtgcattccg tccctgaatc cctgcctgag gaaaaacatt ttaataaaca caaataccgg
     3301 atttgccatc atggagagga aaagcggagt ggacttcgtc tggaaaagtt catttgcaga
     3361 aaggctggag tactgctcca tcttccttag ataatccgca atcgcccggt aggaatagtg
     3421 ctctatgggc tctttcaggt agcccacaga acctttcacc accacttttt catgaacttc
     3481 ccgatactcc at
//
LOCUS       DATKOU010000037        25917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2741828, whole genome shotgun sequence.
ACCESSION   DATKOU010000037 DATKOU010000000
VERSION     DATKOU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 25917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25917
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741828"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VMU21_01485"
     CDS             <1..774
                     /locus_tag="VMU21_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUO76224.1"
                     /translation="TKETDAYMDHLLKKRKKPKTINCHLGCIHAFYEYLIEDEGMPLA
                     NPVKKNYRLRLPRPLPKHLRDEQVLELFKEIKDTRDRAMFMLMLRCGLRVEEVARLTV
                     DAIEYRSRQLCVFNGKGSKDRMVYLSEDAQTALAQYVQKRRASKEKRVFLVQKGPLRG
                     KPISVRGIQKRIEHYAQGAGIEVSCHHLRHTMATQLLNADADLVTIQDLLGHSRITTT
                     QCYCRVSNLKVQRDYFKAIKVVLQRTQPIKGNEYADLTS"
     gene            complement(1101..1928)
                     /locus_tag="VMU21_01490"
     CDS             complement(1101..1928)
                     /locus_tag="VMU21_01490"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HUO76225.1"
                     /translation="MHTSIAGGVHLSLTRARELGCTTMQIFSHNPRQWHVSRMSDEAV
                     RQFKELRELSEIEPVFIHTSYLINLAALRPDILDKSVQLLIREMELADALSAEYVVLH
                     TGSASEDSGDNARRRASDAMRIVSGYRTWNARLLLENTAGERGDISSKIRDLAEIMNA
                     AGSALVAGICLDTCHAFQAGYDLSSEEGLDEIVREIEREIGLERVRLIHLNDSKRAFN
                     AGVDRHEHIGTGEIGKEGLKRIIHHPSLKDVPLILETPKKSEEDDPRNLKVVRGFLK"
     gene            2001..2927
                     /locus_tag="VMU21_01495"
     CDS             2001..2927
                     /locus_tag="VMU21_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HUO76226.1"
                     /translation="MKKKYAIGIDLGGTHLRVGLISEEGEIVQKIKMPSSENIVESLV
                     QSVRDLFSDTVEGIGMGVAGLIDRERRIVTESPNLSRIEGLDIAGTLRNTFSLPVVLE
                     NDANVAAVGEKWMGEGKTFKDFVLLTLGTGIGGGIIHNHGLMDVSAEIGHMSIMANGK
                     HCSCGNYGCLEAYASARAILSHATASLEDSAESALRECCKGNIYKLTPEDIYKAALDG
                     DTLSREILREAGRYLGVGISNLIHVFSPDAIILAGGLIGAWNIFIQEAIKEASRRTFK
                     QLFEKVRILPSSLGDDAGILGASHLVFTCQRT"
     gene            2912..4699
                     /gene="lepA"
                     /locus_tag="VMU21_01500"
     CDS             2912..4699
                     /gene="lepA"
                     /locus_tag="VMU21_01500"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HUO76227.1"
                     /translation="MPKNIRNFSIIAHIDHGKSTLADRLLEYTGALSSREMMEQVLDT
                     MDLERERGITIKAHAVRLNYLAEDGHEYILNLIDTPGHVDFSYEVSRSLVSCEGALLV
                     VDATQGVEAQTVANAHLAMEHNLEMIPVINKIDLPAADPEKIKKQVEDILGIESTDAI
                     LASAKEGIGTEEILEAIVRRIPPPGGDSTKPLKALIFDSWFDNYQGAVVLVRIFEGTV
                     KAGTRMRLMSSGREFEVSQVGIFSPKMEKVDRLSAGEVGYLIAGIKRVGDTNIGDTVT
                     DAMNPSSDACPGYKEIKAMVFCGLYPTETQQYEGLKDALEKLRLNDSSFTFEPETSTA
                     LGFGFRCGFLGLLHMEIIKERLEREFGLSLISTAPTVIYRVTTVKEEVIFIDNPTSLP
                     ERFLTIEEPFVKVTIFVPQAYIGSVLDLCQEKGGTQKEFTYIGKERIMVVYEIPLNEI
                     LWDFYDRLKSVSKGYASMDYEFTGYRESSLTRLDILLNAEPVDALSLIVQKDKAYYKG
                     RQIVEKLREVIPRQLYEVVIQAVIGSKVIARESVKALRKDVLAKCYGGDITRKRKLLE
                     KQKEGKKRMKQVGRIEIPQEAFLSVLKVK"
     gene            4722..5336
                     /gene="lepB"
                     /locus_tag="VMU21_01505"
     CDS             4722..5336
                     /gene="lepB"
                     /locus_tag="VMU21_01505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HUO76228.1"
                     /translation="MKKNKKELVWEYAKAVVTALLLALFIRAYFIQAFKIPSGSMIPT
                     LLIGDHILVNKFIYGTTIPFSDKRILVFRKPEKGDIIVFKYPEDRSRDFIKRVMAMEG
                     DVIESKNKTIYVNGKAVDEPYAQHTDTSLRPGGIEPRDNFGPYIVPKGKYFMMGDNRD
                     QSYDSRYWGYVDLTDIRGEALILYWSWDNKNHWPRFERIGRLVK"
     gene            5333..5680
                     /locus_tag="VMU21_01510"
     CDS             5333..5680
                     /locus_tag="VMU21_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76229.1"
                     /translation="MRYLCNSDGFIKPLLVIAIMAFLIYAGMQFGIPYYRASAFKSEV
                     KEIARLELGQIEKTRAQIFEVAQNQKIPIEAEDIQITKKTNTTRVQTSWSVDVDILGL
                     YQRRLQFTVDVEE"
     gene            5682..6344
                     /locus_tag="VMU21_01515"
     CDS             5682..6344
                     /locus_tag="VMU21_01515"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HUO76230.1"
                     /translation="MFTGLIVELGTVVSLRRKEPGAFLTLSADTLAKDAGIGDSIAIN
                     GVCLTVVAREGSMLSFDLSDETVRSTNFGLLKQGDRVNLEPSLRSDGRLGGHFVTGHV
                     DAVGKIRSKTIVGDTVKVVIDSPGEVARLLVEKGSVAVDGISLTVVNAARDSFSVVII
                     PHTSRLTTIGFKNAGDTVNLEADIIGKYVAKFLQGYSMDRGNSDSRLVRSLKASGYIQ
                     EE"
     gene            6348..7559
                     /locus_tag="VMU21_01520"
     CDS             6348..7559
                     /locus_tag="VMU21_01520"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HUO76231.1"
                     /translation="MHKHKFNTIDEAIEDIANGKMVILVDDEDRENEGDLCMAAEKVT
                     PAAINFMAKYGRGLICLSLPPQRVEELQLPMMTDENTSQFGTAFTVSIEAKKGVTTGI
                     SASDRATTILTAIDPKMGSEDLARPGHVFPLRAKPGGVLQRAGQTEGSVDLARLAGLY
                     PAGVICEIMSDDGTMARVPELIEFAKRHTQKIVTVKDLIHYRTRTERFVKRISDVNLP
                     TDHGEFRAVAYANDMDSNIHIALVKGEIKPEEAVLVRVHSECLTGDVFGSKRCDCGEQ
                     LHKAMEMINKEGKGVILYMRQEGRGIGLANKLKAYELQDKGLDTVEANIKLGFKPDLR
                     DYGIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGLKVVERVPVESSPHERNIIYLRTK
                     KKKLGHMLDNV"
     gene            7619..8239
                     /gene="arsM"
                     /locus_tag="VMU21_01525"
     CDS             7619..8239
                     /gene="arsM"
                     /locus_tag="VMU21_01525"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HUO76232.1"
                     /translation="MDREKAKIKQLVIKSYGAVATGSPSCLPQTSCCAPGAGAQTLLE
                     TGRRLGYSDEELTVGLGEANLGLGCGNPHAIADLKKGETVLDLGSGPGFDSFLSCMKV
                     GKTGSVIGIDMTPEMVAKARENAQKLGLKNVGFRLGEIEHLPLENASVDVIISNCVIN
                     LSPDKQAVFNEALRVLKPGGRLAISDVLKKGEFSEAIKQNEHAYSG"
     gene            8258..8413
                     /locus_tag="VMU21_01530"
     CDS             8258..8413
                     /locus_tag="VMU21_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76233.1"
                     /translation="MGELKEILGKAGFIDIAIQDKDNSDEIIKSWNFGEGVEKMVFSA
                     YITAKKP"
     gene            8452..9348
                     /locus_tag="VMU21_01535"
     CDS             8452..9348
                     /locus_tag="VMU21_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76234.1"
                     /translation="MSEDEIQDEVLHLKAEIKRLRKSLAELTPPLPLVLKRRGFKIFK
                     KEPCDDLLLPEERFIDEYYRLLQKYSFRLFLRDVIHHQHHFTIRDVTRYSTQEVTEEY
                     VSLLLRIRLIEREGGTDSYRLRKGPVKSFGPTLEWFLSEIFKSEFAAEAVWGVKFKRP
                     VVGGDYDVIAKVDSSLLSMEVKSSPPKQIYAKEISAFFDRLFDLIPDIAIFFMDTELR
                     MKDKIVPMFEDEIKRRSSHELSSFLDIPVSPQEPPPVVRMEKELFQIQNKIFIINAKD
                     SIVNNLEKVLRRFFRRSQRPVK"
     gene            9484..10842
                     /locus_tag="VMU21_01540"
     CDS             9484..10842
                     /locus_tag="VMU21_01540"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HUO76235.1"
                     /translation="MNLRELKNTLSVVGGKIQIHDNDALRKAMDELVFQAVFTENDDT
                     KRDLLIFIKEAAKTAGAFPASIQGLYEELGREYRGFTVPAMNIRGLTYDTARAVFRKA
                     MEKNVGAFIFEIARSEIGYTKQRPLEYSAVVLAAAVREGFSGPVFIQGDHFQLVRKSY
                     LADPIAETTYVKGLIKEAVEAEFYNIDIDSSTLVDLEKQTIKEQQRPNFEKTAELAQS
                     IREIQPAGIDISIGGEIGEIGGKNSSTDELRAYLDGFRETFRSAGKGVSKLSVQTGTS
                     HGGVVLPDGTLAKVKIDFDTLKTLSEIARKEYGLSGAVQHGASTLPEEAFDMFPKTGT
                     SEVHLATGFQNIVYESKHLPEEFREEVYAFIKQEYSKEWKEGQTEEQFIYSTRKKGFG
                     PLKSKWWQLPGEVKEPIMKALEAQFGLLFEKLKVTRTMDIVKKTVKPAIVAKEINPRL
                     QD"
     gene            10877..12091
                     /locus_tag="VMU21_01545"
     CDS             10877..12091
                     /locus_tag="VMU21_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HUO76236.1"
                     /translation="MPDIKRIGVITSGGDAGGLNAVIKGVAREAYAHGIESVVIPNGY
                     AGLYNLIDLQNVVVLNAERVSRIEASLSGSEAGHSRVKISKISDPQRYERIKEGLKKF
                     GIDALIISGGDDTGSVVVDLGAQGIPCVHVPKTMDLDLQPYSVGGDSAINRIAVMVRD
                     LKTTGMSHNRVMVVEVFGRYVGHTALRGGIGAEADCILIPEVPVDFDVVYEHIKATYF
                     ARIERSDVKAGTYMMVVAEGIKTASGELLYDESAGVDAFGHKKLAGAGRYVRQQIEKR
                     LKADPEVKEFMKRTGMYAPGVYEIPEVREVAPSHLVRSGQTSAYDVNFGYRTGAAAVL
                     LLLEGKTGNTVVDVNGNKISYMTTSEAIKRREVDLSEIALFETLGICFGRKPQKFEYE
                     TMAAKGTVERHL"
     gene            12123..13385
                     /gene="pfp"
                     /locus_tag="VMU21_01550"
     CDS             12123..13385
                     /gene="pfp"
                     /locus_tag="VMU21_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HUO76237.1"
                     /translation="MSKKLGNKIAIIVGGGPAPGINGVISAATIEAIALGMTVIGISG
                     GFKSLFDGNRDCVIPLTIDEVSRIHTYGGCILRTSREYPEMVKEKFQVLMSTLKRIGA
                     THLLTVGGEGTLFMANWIERESRGSISVVHVPKTIDNDIPLPGGVSTFGYQTARHRGV
                     EIVENIMEDAKTTGRWYFITTMGRHTGHLALGIGKASGATVSLIPEEFEGETLSFKKV
                     ADILTCSIIKRLSMHKDHGVAILGEGIAEKFDMDELTHYEQLESDETGRVRLSEIQLG
                     RVMKNFVKKTLESMGIRITIVDKNIGYELRAANPIPYDVEYTRDLGYGAVNYLHKGGT
                     GAMIVVYEGRLVPVPFVELIDYSTGKVKIRMVDITSETYTVARKYMIRLERDDFSPQN
                     LKKLAKSANLNTSEFRDRFGYLVGPPEM"
     gene            13435..14163
                     /locus_tag="VMU21_01555"
     CDS             13435..14163
                     /locus_tag="VMU21_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUO76238.1"
                     /translation="MPALNIGCSGFLYDHWKGAFYPEDLPKRRWFEYYCTKFSTVELN
                     VTFYRLPDKETFTKWYKETPPGFVFSLKGSKFITHIKKLKAAAEPVDVFFSRALALKE
                     KLGGVLWQLPPGLKADPGRLAEFLELLKPYGVRNTFEFREGTWVSKKVFTLLEKRNAC
                     LCMADWPEFLDDLPLTADFTYVRRHGKEGSYATCYSAEELKADATRIRRYLRQRKDVY
                     VYFNNDALGYAPKNASELITMMKK"
     gene            14274..16016
                     /gene="polX"
                     /locus_tag="VMU21_01560"
     CDS             14274..16016
                     /gene="polX"
                     /locus_tag="VMU21_01560"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HUO76239.1"
                     /translation="MINQQIARIFNEMADLLEIKGENPFRIRAYRRAAQNVEGLPKDV
                     AQMTEDEILEIPGIGKDLAGKIRHYADSGRMEAYEELKSLVPGGLAALISVPGLGPKT
                     AKLLYDRLKIKGIDDLERLARAHRLSGLPGIKDKTEENILKGIEMMRRGKERLPIGRV
                     LPIANEIIAWLREKAPVQELVLAGSLRRWKDTIKDIDILATSGTPGKVMGLFVHLPMV
                     KEILMRGPTKSSVLTTEGIQVDLRVVDKDSFGAALAYFTGSKAHNIRIREMAVKAGLK
                     INEYGIFREKDSARLGGKDEKDVYRLLGLPSIPPELREDTGEIEAAMEGRLPKLLELS
                     DIRGDLHVHTKRSDGSHTLEELVDAARSRGYQYLAVTDHTKGLGIARGLNEDKIMEQM
                     KEIKKVNSRMRGFRVFMGAEVDIRSDSTLDLPDEILRQLDIVVASIHSGFKQSREQLT
                     RRLVAAIQNPYVSVIAHPTGRLIGERDAYEVDMERVFAALKESGKAVEMNAYPLRLDI
                     NDTLAKKAKEMNIPVAISTDTHVLSQFDYMVYGVSIGRRGWLEKNDVLNTLHTGNLLK
                     WLQRSKLSHLRAER"
     gene            16021..17412
                     /locus_tag="VMU21_01565"
     CDS             16021..17412
                     /locus_tag="VMU21_01565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUO76240.1"
                     /translation="MILVYEKDQGTLKSLRSFFKKRKYLKAEFIEDMTSLAARTSRIK
                     GKNLICIIAAGELPKLKPRELNCSFIATVSGASRGVIRKALDYGAESYLITPLHEEDL
                     EQKIRDVIKENQERQRMRKETHMLQTIIDISGHISSTLDPKEILYLIVSNISEIIPVT
                     RCSIISVDGGQRYAHVISTFEDPHLTNIKLDLNKYPEIREALSSKKPVAIKNAAKDPI
                     MKRVSEILFPLGIRSILVIPIIFHEEIIGTLFLRTSRARHAFTRHEINLCQAIANASA
                     NPLYNAYLFEKMGDEKSHLEKLAVTDYLTGLYNNRYFYYRLAEEYSRSVRHSFHLSCL
                     MLDIDFFKEVNDRYGHKAGDNILKELAQLLKHHMRKSDVVARYGGEEFIILLPQTDAN
                     GALAKAESLRSHIENHPFRGLKGEKGLSVSIGLATHPDHRAPNKEDLITLADNALYTA
                     KAMGRNQVVFSKP"
     gene            17488..20106
                     /locus_tag="VMU21_01570"
     CDS             17488..20106
                     /locus_tag="VMU21_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO76241.1"
                     /translation="MDIITSHTNADFDAFASMVAARKLYPGALLVFPGSQEKKVRDFI
                     AAFQPVEISRVKEIEIAQVRRLILVDTKSSERIGPFAELAAKSAQTEIHLYDHHPRTK
                     DDIRGSMEVIEDVGATATILTEILKSRKIPITPMEATMLCLGIYEETGSLLFPSTTER
                     DMIAAAYLLRRGASLKIVSNFIRTELSKEELDILNELLRSSTHLEMNGTRVMIAKASR
                     EDYLGDAAHLAHRIMDMEDTDGLVLILSMQGKILMVGRSRVPEFDVAEIMKKFGGGGH
                     PNAASATLKETPLEVLEDEVKSLLKSSVRYGKSAKDVMTTPVITISWTDAVKDAESMM
                     TRYGVNVLPVVMDGKYNGIISREIVEKAIFHGFGRSRISDFTTTDAQTVSHDTQVRDI
                     ERLMIEQNQRFMPVLEGERIIGAITRTDILRILYEDMLKRSRVQSPAGEKPSMGRNLA
                     PWLKEKFPPAIAEILKLSGEVAESLGSKAYLVGGSVRDLLRGQENLDIDLVIEGEGIP
                     FAREVAKRLGAKVRTHERFGTAQVITDHLKLDVATARTEYYESPAALPKVETSSIKKD
                     LYRRDFTINTLAISLNPRDFGLLIDFFGGQRDLREKTIRVLHNLSFIEDPTRAFRAVR
                     FSERFGFKLSKHTENLIKSALRMNLFDRLSGSRLYEELILTFSETEPVKAIKRLSQYG
                     LLKVIHQNLAFDERLDSMLQSIHDTLSWFNLLFLEEKPDKGIIYMMALLSVLGDGDRG
                     NAFIRLSTPPRVKERINKGILHSQELVKLLPLHDAAKVYKALYPLDLESVLFSMATAA
                     NDNKKRELSHYLIELRRVKPQLTGQDLKNMGIMPGPVYSEILDTLLEERLRRNLKTRE
                     DELAFVKERYKTISHY"
     gene            20188..20418
                     /locus_tag="VMU21_01575"
     CDS             20188..20418
                     /locus_tag="VMU21_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HUO76242.1"
                     /translation="MARVYLLVNVLPGKDVFIRDTLRGIRGVVSADVVTGHYDIAAVI
                     EGKDTGEIFDKIFQKIRKIKGINRTESFVAVE"
     gene            complement(20509..21390)
                     /locus_tag="VMU21_01580"
     CDS             complement(20509..21390)
                     /locus_tag="VMU21_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HUO76243.1"
                     /translation="MKWLAPLLISFLAFTLSASGALAQQLNVVASISPIGDMVREVGG
                     DRVAVRVLLPPGASPHVFEPTTGTAKDISQARLFFGVGAGLDFWAEKLVRASRETIRI
                     ILLSEGMKLLHEEEDHDHGHGHETANPHVWLDPLLAMEMVRKIEKTLQETDPQGGYTY
                     HVRSAEYIAKLQALDAEIRKNVSTFTIKSFVSFHPAWDYFALRYGLKSVGVIEESPGK
                     EPSPQRLQAIVKAIRHYHIRAVFAEPQLNPKTAEVIAHEAGVKVIMLDPEGGLPGRET
                     YLGLMKYNLARLKEAMQ"
     gene            21636..22913
                     /gene="tyrS"
                     /locus_tag="VMU21_01585"
     CDS             21636..22913
                     /gene="tyrS"
                     /locus_tag="VMU21_01585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HUO76244.1"
                     /translation="MVKPEKQLEIIKRGSVEIILEKDLFLKLERSYKDNSPLRIKAGF
                     DPTAPDIHLGHTVLLEKMRQFQELGHEVIFLIGDFTGMIGDPSGKSETRKPLTKEEVL
                     RNAETYKTQVFKILDQGKTRMLFNSEWLGKMESMELVRLGAMQTVARMLEREDFKKRF
                     KNQQDISILEFYYPLFQAYDSVHLKADVELGGTDQKFNLLMGRTLQRKMSIEEQVVIM
                     MPLLEGTDGVNKMSKSLGNYVGIHEPAFEFVHGEATGMFPKIMRIPDKLIIRYLELLS
                     HISMNELSALRQREQEGSLDYLKAKSDLAMEIVARYHGKQEAERAREEFEHVRDKKLP
                     DDIPTITITLEGGSVAGGTLKVELKTRKTWLPHIMKTTGLSKSTSEAIRLIQQGGVKI
                     NDHTITDSNAELHKGEHIIKVGKRKFYKVKIQS"
     gene            22910..23581
                     /locus_tag="VMU21_01590"
     CDS             22910..23581
                     /locus_tag="VMU21_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HUO76245.1"
                     /translation="MISNAEIILRLLIGAVFGGIVGFERHAHGRAAGFRTHLLVCVAS
                     VLAMIVSEYYHYLSLFDPSYVRADPGRIAAGAITGMGFIGAGVIVKMGFNVQGLTTAA
                     CLWIVSIVGLSIGSGLYVAGTTGFVITYLALWLLRQWEERMPGLSYKFVNVVMQGMGD
                     EESINLAIKRCGALIEKTDYERDLEHKEATYNITITFKGEGCTEKLLAELSSLQNVKR
                     VMIRS"
     gene            complement(23565..25142)
                     /gene="cobA"
                     /locus_tag="VMU21_01595"
     CDS             complement(23565..25142)
                     /gene="cobA"
                     /locus_tag="VMU21_01595"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HUO76246.1"
                     /translation="MRKDKGKVYLVGAGPGDIGLLTVKGMKCLQKAEVVVYDFHLNAQ
                     VLNYINHDAEFIYAGKRGGHHTLKQDEINELLVEKAREGKLICRLKGGDPFIFGRGGE
                     EAEVLARNGITFEVVPGVSSAVAAPAYAGIPLTHRLYSSSFAVIPGYEDTTKQGSSID
                     WAKLSTGVGTLVVLMAVKNLDLLVQKLIENGKDPGTPVAVVRWGTRADQRTIVSTLRD
                     IVSEIKDKEIKPPAVCVIGEVVRLRDSLRWYEDKPMFGHRVLVTREHSSGFEPLEELG
                     AEIVEFPTIEIVPPLSYKEMDAAIKNIQSYQWIIFTSANGVRYFLNRFVEKDRDIRDL
                     KGIGICAIGAKTASEINKYGIKVDLVPDEFHAEGLIEAFVKEVKSQKSKAKSLGPELL
                     KGMKFLLPRAEIAREIFPEKVRELGGEIDVPVAYRAVKPELHGKRLKRFLKEGRVTIA
                     TFTSAATFKNFVEIMGSDAHELLKGVTIAVIGPVTAKAIEKAGLKVDIMPKESTIEAL
                     VSEIIRAKAKGNVNCES"
     gene            25342..>25917
                     /gene="dgt"
                     /locus_tag="VMU21_01600"
     CDS             25342..>25917
                     /gene="dgt"
                     /locus_tag="VMU21_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HUO76247.1"
                     /translation="MTIREQTEEIERKTLHPRACLSSGSKGRVTPEKEGEIRTCFQRD
                     RDRIIHSKAFRRLKHKTQVFLAPRGDHYRTRLTHVLEVSQIARTIARALRLNEDLTEA
                     IALGHDLGHTPFGHAGESVLREIYPGGFDHYKQSLRVVDLLERNGQGLNLTHEVRNGI
                     VTHSKGKGLIIPEKRSERAETLEGLAVRISDV"
BASE COUNT         7055 a         6099 c         6876 g         5887 t
ORIGIN      
        1 accaaggaaa ccgatgcgta tatggaccat ctattgaaaa agagaaaaaa gcccaagacg
       61 atcaactgtc atctcggctg catccatgcc ttttacgaat accttatcga agatgaggga
      121 atgccgcttg ccaatccggt caaaaagaat tacagacttc gtctgccccg gccgctgccc
      181 aaacatctca gagacgaaca ggtgctggaa ctgttcaaag agatcaagga taccagagac
      241 cgggccatgt tcatgctcat gctcagatgc ggtctgaggg tcgaagaggt tgcacgactg
      301 acagtggatg cgatagagta ccgcagcaga cagctgtgcg tcttcaatgg caaaggcagc
      361 aaggacagaa tggtgtatct gagtgaggat gctcagaccg ccttagcgca gtatgtgcag
      421 aaacgcagag cgtcgaagga gaagagagtg ttcctggtgc agaagggacc gttgagggga
      481 aagccgatat cagtgcgcgg cattcaaaag aggattgagc actatgcaca gggggccggg
      541 atagaggtat cctgtcatca cctcagacac accatggcaa cccagctgtt aaatgcagat
      601 gccgatcttg tcaccataca ggatctgctg ggccacagcc gtattactac aacccagtgc
      661 tattgtagag tctccaatct caaagtacag agagactact tcaaggccat aaaggttgta
      721 ttgcagcgga cccagccaat aaagggtaat gaatacgcag atttaaccag ctgaaaataa
      781 aagaaaaagc aggttatgtt acgtatagca tggtggaccc aacaccaacc aatgaaaatg
      841 ctcccttaac ctttttgaga ctactatcac tcaatgtttc tttcaatatt aaacacgcca
      901 gagatctgag agataagatc tgctaaagtt actttctcct ccgcagtgtg tatacacctc
      961 acaaagttac acggagaagt ctttggtaac aaaaaaatga gtctagcgct gatttctgtg
     1021 agttaatacg attgtctcga aatacattga aaccctaaca cacccgcaca aacgcgcgaa
     1081 aagttaacac gtaacaccgc ttatttcaaa aaccctctca ccactttcag gtttctcggg
     1141 tcatcttcct cgctcttctt gggagtctcg aggatcaggg ggacatcttt taatgaagga
     1201 tgatggatga tccgtttcag cccctccttc ccgatctccc ctgtcccgat gtgttcatgc
     1261 ctgtcaaccc ctgcattgaa agccctcttc gaatcattga gatgtatgag tctgaccctt
     1321 tctaacccaa tctcccgttc aatctcccgc acaatctcat caagcccctc ctcactcgaa
     1381 agatcatatc ctgcctggaa tgcgtgacaa gtatccaggc atattccagc cacgagcgca
     1441 ctgcctgcgg cattcatgat ctccgccaga tccctgatct ttgatgagat gtcgcccctc
     1501 tcccccgcgg tattctcaag gaggaggcgt gcattccatg tacggtaacc ggaaacaatt
     1561 ctcattgcat cactcgctct cctccgggca ttgtccccgc tgtcctctga tgcgctgcct
     1621 gtatgaagga ccacatactc ggcactcagg gcatctgcca gctccatctc cctgatgagg
     1681 agctgcactg atttatcaag aatatcggga cgtagggcag caagattaat aaggtatgac
     1741 gtgtgtatga aaaccggttc gatctcactc agttccctca gttccttgaa ttgtctcacc
     1801 gcttcgtctg acatcctgct cacatgccat tgtctcgggt tatgagaaaa tatctgcatg
     1861 gtggtgcacc ccagttccct tgcccttgta agggacagat gcacgccgcc tgcgattgat
     1921 gtgtgcacgc cgagtcttct caccgaatga gccctttatg gtataattat aaatcttctt
     1981 cagttgtcct gctcaaaggt atgaaaaaaa aatacgcaat cggcatagac ctcggaggaa
     2041 cccatttgag ggtaggctta atttccgaag agggagaaat tgtccaaaag atcaaaatgc
     2101 cctcatctga gaatattgtg gaatcccttg tgcagtccgt ccgcgacctg ttcagcgaca
     2161 ccgtagaagg aatagggatg ggtgttgcag ggctcattga cagggagagg agaatcgtca
     2221 ctgaatcccc caatctttcc aggattgaag gactggacat cgccggtacc ctaagaaata
     2281 ccttcagtct cccggtggtt ttggaaaatg acgccaatgt ggctgccgtg ggagagaaat
     2341 ggatgggtga aggaaagacc ttcaaagact ttgtgcttct gaccctcggg acagggatcg
     2401 gtggcggcat cattcataac cacggtctca tggacgtttc agccgagata ggacatatga
     2461 gcataatggc gaatggaaaa cattgttcct gtggaaacta tggctgtctt gaggcatatg
     2521 cttctgcaag ggccattctt tcccacgcca ccgcatccct ggaagacagc gctgaaagcg
     2581 cgttgcggga atgctgcaag gggaatatct ataaattgac acctgaagac atctataagg
     2641 ccgcccttga cggtgacacc ctttccaggg aaatcctgag ggaggcgggg agatatctcg
     2701 gcgtgggaat atcaaacctg attcatgtat tcagccctga cgcaatcata cttgcaggag
     2761 gcctcatcgg ggcctggaac atctttatac aggaagcgat caaggaggct tcacgacgca
     2821 ccttcaagca gctgtttgaa aaggtgagga ttctcccttc gtcccttggc gacgatgcgg
     2881 gtatccttgg tgcctcgcac cttgtcttta catgccaaag aacataagaa atttttcgat
     2941 aatcgcccac atcgaccacg gcaagtccac cctggctgac aggctcctcg aatacacagg
     3001 cgccttgagt tcaagggaga tgatggagca ggtcctggac accatggacc tcgagaggga
     3061 aagaggcatt accataaagg cccatgcggt ccggctcaat tacctggcag aggacggaca
     3121 tgagtacata ctcaatctca ttgatacccc cggccatgtg gacttttcct acgaagtttc
     3181 ccgaagcctt gtctcgtgcg aaggggccct cctcgtggtg gacgcgaccc agggcgtaga
     3241 agcacagaca gtggcaaatg cgcaccttgc catggaacac aatctcgaga tgatccctgt
     3301 tatcaataaa atcgaccttc ccgcagcaga ccccgagaag ataaagaagc aggttgagga
     3361 tattctcgga atagagagca cggatgccat actcgccagc gcaaaggagg ggattggcac
     3421 cgaggaaata ctggaggcca ttgtcaggag gattcccccc ccgggcggag acagcaccaa
     3481 gcccctcaag gccctcatat tcgactcctg gtttgataat taccagggcg cggtagtcct
     3541 tgtgagaata ttcgaaggaa cggtgaaggc cgggaccagg atgcggctca tgagtagcgg
     3601 tagggaattt gaagtgtccc aggtgggaat attttccccg aagatggaga aagtggacag
     3661 gctttctgca ggagaggtgg gatatctcat cgccggaatc aaacgggtcg gtgacaccaa
     3721 catcggggat accgttaccg atgcgatgaa tccctcaagc gatgcgtgtc ccggatacaa
     3781 ggagataaag gccatggtgt tctgcggcct ctatcccacg gaaacccagc agtatgaggg
     3841 cctcaaagac gccctggaaa aactccgcct caatgactcg tcctttacct tcgagccgga
     3901 aacctccact gcccttggct ttggattcag atgtggtttc ctggggctcc tccacatgga
     3961 gatcatcaaa gaacggcttg agcgggagtt tggtctctcc ctcatcagca ccgcacccac
     4021 tgtcatctac cgggtgacga cggtaaaaga agaggtgata tttattgaca accccacatc
     4081 cctcccggaa cggtttctca ctattgagga acccttcgtg aaggtcacca tattcgtccc
     4141 gcaggcatat attggctctg tccttgacct ctgtcaggaa aagggcggga cacagaaaga
     4201 gtttacctat atcggcaagg agaggattat ggtggtgtac gagatccctc tcaatgagat
     4261 tctgtgggac ttctacgaca ggctgaaatc agtatcaaag ggatatgcgt ccatggatta
     4321 tgagttcaca ggatacaggg aatcaagcct cacgagactc gatattctgt tgaacgctga
     4381 gcccgtagac gccctttcct tgatagttca aaaggacaag gcgtattata aaggcaggca
     4441 gattgtggaa aagttaaggg aggtcattcc gaggcaactt tacgaggtgg tgatacaggc
     4501 agtaatcgga agcaaggtga ttgcgaggga gagtgtaaag gcactccgca aggatgtcct
     4561 tgcaaagtgt tatggcggtg atatcacaag aaagagaaag ctcctggaaa aacaaaaaga
     4621 agggaagaaa cgcatgaagc aggtggggag gatagagatt ccccaggagg catttctgtc
     4681 agtattgaag gtaaaatgaa gcgacaggag gagttccgca agtgaagaag aacaaaaagg
     4741 agttagtatg ggaatatgca aaggcagttg tcacggccct cttacttgcc ctctttatca
     4801 gggcatattt tatccaggca tttaagatcc cctcgggatc catgatcccg acgctgctca
     4861 taggcgacca catccttgtg aataaattta tttatggcac aacaatcccc ttctctgaca
     4921 agagaatcct tgtcttcagg aagccggaaa aaggtgatat aatagtcttc aaatatcccg
     4981 aagaccgcag cagggacttt ataaaaaggg tcatggcgat ggagggtgat gtaattgaat
     5041 cgaagaataa gacgatctat gtgaacggga aggccgtgga tgagccttat gcccagcaca
     5101 ctgacacttc cctgaggccg gggggtatcg aaccgaggga caatttcgga ccgtatatcg
     5161 tcccaaaggg aaaatacttt atgatgggag acaaccgcga ccagagctat gacagcagat
     5221 actggggcta tgtggatttg acggatatca ggggagaggc actcattctt tactggtcgt
     5281 gggacaataa gaaccattgg ccaagatttg aaaggatagg gaggttggtg aaatgagata
     5341 tctttgtaac agcgatgggt tcataaagcc gctgctggtc atcgcaatca tggcgtttct
     5401 catttatgcg ggtatgcagt tcgggatccc ctactaccgc gcttcagcct tcaagagcga
     5461 ggtaaaggag attgcgcgtc tcgaactggg gcagattgag aaaacaaggg cccagatttt
     5521 tgaggttgca cagaatcaga agatacccat tgaagcagag gatattcaga ttactaagaa
     5581 aacaaatacc acccgtgtcc agacctcatg gtccgtggac gtggatatcc ttgggctcta
     5641 tcaaagaaga cttcagttca cggttgatgt ggaggaatga ggtgtttaca gggctcatcg
     5701 ttgaattagg gactgtggtg tccctaagaa ggaaagagcc cggggctttc ctcacgcttt
     5761 ctgcagatac cttagcaaaa gatgcaggta tcggggacag cattgcgatc aatggggtgt
     5821 gtctcactgt ggtggcgcgt gaaggttcaa tgctttcttt cgacctctcc gatgagacgg
     5881 tgcgttccac aaacttcgga ctgctcaagc agggagacag ggtaaatctt gagccctccc
     5941 tcaggtctga tgggagactc ggcggacatt ttgtgacagg ccatgtggat gccgtgggaa
     6001 agatcaggtc gaaaactata gtgggagata ccgttaaggt ggtaatcgat tcccctggag
     6061 aagtggccag gcttctggtg gaaaagggtt ctgtggcggt agacgggata agcctcacgg
     6121 tggtgaacgc ggccagggac agtttctctg tggtgataat cccccatact tcccgtctta
     6181 ccaccatcgg gttcaagaat gcgggtgaca ctgtgaacct cgaggcagat attataggga
     6241 agtatgtggc caagtttctc caagggtaca gtatggatag ggggaatagc gattcacgat
     6301 tggtcaggtc cctgaaagcg tcgggatata ttcaggagga gtagaccatg cacaagcaca
     6361 aattcaatac catagacgaa gccatagaag acattgcaaa cggtaagatg gtcatcctgg
     6421 ttgacgacga agacagagag aacgagggag acctctgcat ggcagcggaa aaagttactc
     6481 ccgctgccat aaacttcatg gcaaaatacg gaaggggtct tatctgcctc tccttgcccc
     6541 cccaaagggt tgaggaactg cagcttccca tgatgactga tgagaatact tcccaattcg
     6601 gtactgcctt taccgtgtct attgaggcga aaaaaggcgt gactacgggg atctcggcat
     6661 ccgacagggc aaccaccatt ctcactgcca ttgatcccaa gatgggatct gaggaccttg
     6721 caagaccggg gcatgtgttc ccccttcgtg caaaacccgg gggagtgctc cagagggctg
     6781 gccagacaga agggtctgtg gaccttgcga ggcttgcagg actttacccg gcaggtgtca
     6841 tctgcgagat catgagcgat gacggtacca tggcacgtgt tcctgaatta atagagtttg
     6901 cgaaacgcca tacccagaag atagtcacgg taaaagacct tattcactac aggaccagga
     6961 ccgaacggtt tgtgaaaaga atctcggatg tcaacctacc caccgaccat ggcgagttca
     7021 gagccgttgc ctatgcgaac gacatggaca gcaatatcca tatcgccctt gtgaaaggag
     7081 aaattaagcc tgaggaggct gtgctggtga gggtccattc agagtgtctt acaggtgatg
     7141 tcttcggttc aaagcggtgt gattgtggag aacagctgca taaagccatg gaaatgataa
     7201 ataaggaagg gaaaggggtc atcctctaca tgaggcagga gggaaggggt attggcctcg
     7261 caaacaagct taaggcatat gaacttcagg acaaaggtct ggatacggta gaggcaaata
     7321 taaagctggg tttcaagccc gatttaaggg attatgggat tggcgcgcaa atactcgttg
     7381 acctgggggt gaggaagatg cggctcatga caaataaccc caagaaaatc gtcgggctgg
     7441 agggatatgg tctgaaggtc gttgaaaggg tacccgtgga gagcagtccc cacgaacgga
     7501 atattatcta cctcagaaca aaaaagaaga agctcgggca tatgctggac aatgtataac
     7561 accatggtgt tctatttctt tcaagcacat ctcattctgc acagtgcggc ggtttcccat
     7621 ggatagagaa aaagcaaaga ttaagcagct tgtgataaag agctatggcg cggtggcgac
     7681 gggtagcccc tcgtgtcttc cccaaacaag ctgctgtgct ccgggggcag gcgcgcaaac
     7741 ccttctcgaa acaggcagga ggcttgggta ttcagacgaa gaactcacgg tcggactggg
     7801 ggaggcgaac ctcggcctcg gctgcggaaa tccccatgca atcgcggatc tcaaaaaagg
     7861 agagaccgtg ctcgacctcg ggagtggtcc ggggttcgac tcctttcttt cgtgtatgaa
     7921 ggtggggaaa acagggtctg ttatcggtat agacatgacc cccgaaatgg tggcaaaggc
     7981 aagggaaaat gcacagaaac tcggcctgaa gaatgtgggg tttaggcttg gagaaatcga
     8041 acatcttccc cttgaaaatg catctgttga tgtaatcatc tccaattgcg tgatcaatct
     8101 ttctcccgat aagcaggcgg tgttcaatga agcactcagg gtcttgaaac cgggcgggag
     8161 actcgcgatc tctgacgtgc tgaaaaaagg tgaattttcc gaggctatca aacagaatga
     8221 gcacgcctac agtggctgaa ttaccggttc agttcccgtg ggggaactga aagagatcct
     8281 cggaaaggcc ggatttatcg atatcgctat tcaggacaag gacaacagtg atgagattat
     8341 taagagctgg aatttcggag aaggggttga gaagatggtt ttctcagcct atatcactgc
     8401 caagaaaccc taaacgcgac gctgctctat tcagcacaat gtcttgagta gatgagcgaa
     8461 gatgagattc aagatgaggt tctgcatctc aaggccgaaa taaaaaggct tagaaagtca
     8521 cttgcagagc tcacccctcc cctcccctta gtattgaaga ggaggggatt caagatcttc
     8581 aaaaaagagc cgtgtgatga ccttctgttg ccggaagaac ggttcattga tgaatactac
     8641 cggctgctcc agaagtactc attcaggctt tttctcaggg atgtgattca ccaccagcac
     8701 cacttcacca taagggatgt taccagatat tctacccaag aggtgactga agagtatgtg
     8761 tcacttctgc tcaggatcag gctcattgaa agggaagggg gaacagattc ataccgcctc
     8821 aggaagggac ctgtcaagag ttttgggccc acccttgaat ggtttctctc cgagatattc
     8881 aaaagtgagt ttgctgctga ggctgtatgg ggagtaaagt tcaaaaggcc tgtggtgggg
     8941 ggagactatg atgtgattgc taaggtggat agctcccttc tgtcaatgga ggtcaagtct
     9001 tctcccccaa agcagatata cgcaaaggaa atctccgcct ttttcgacag gcttttcgac
     9061 ctcatccccg atattgcgat attttttatg gatacagagc tgaggatgaa ggacaagatc
     9121 gttcccatgt tcgaagatga gatcaaaaga cggtcatccc atgaactttc ctcttttctg
     9181 gatattcctg tctctcccca ggaacctcct cctgtggtgc gcatggaaaa agagctcttc
     9241 cagatacaga acaaaatctt tataataaac gcaaaagata gtatagtgaa taaccttgaa
     9301 aaggtgttgc gccggttttt caggaggtct caacggccgg tgaaatagag atatgtgcca
     9361 gatgtgcgtg gagagggtca tgccggaaaa agttttctgt ttccggcacg tagctccgtt
     9421 gcactgattt tgttaagtat atttccctaa acgcagaaga cgaaaagagg agggctagaa
     9481 agtatgaatc tgagggaact aaagaacacg ctgtcggtgg tcggggggaa gatccagata
     9541 cacgataatg atgcgttacg aaaggctatg gacgaactgg tattccaggc ggtatttacg
     9601 gaaaacgatg acacaaaaag agacctcctc atattcatta aagaagcggc aaagaccgct
     9661 ggcgcgtttc ccgcttctat ccaggggttg tatgaggaat taggccgtga gtatcgcggc
     9721 ttcaccgtgc ctgccatgaa catcagaggg ttgacctatg ataccgcacg ggcagtgttc
     9781 aggaaggcca tggagaagaa tgtgggcgcc tttatcttcg aaattgcacg atccgagata
     9841 gggtacacaa agcagaggcc ccttgagtat tccgcagtcg tattggctgc cgcggtaagg
     9901 gagggattct caggccccgt ttttattcag ggtgatcact ttcagcttgt gagaaagagc
     9961 tatctcgcgg accccattgc agagaccacc tatgtcaagg ggctgatcaa agaggcggtt
    10021 gaggcggagt tctataacat cgatatcgat tcttccaccc tcgttgacct ggagaaacag
    10081 accatcaagg aacagcagag accaaacttc gaaaaaaccg ctgaactggc gcaaagtatt
    10141 agggagattc agcccgcggg tatcgacatc tccatagggg gcgagattgg ggagatcggc
    10201 ggcaagaaca gttccactga cgaactcagg gcgtacctcg atggattcag ggagaccttc
    10261 aggagtgcgg gtaaaggcgt cagcaagctc agtgtccaga cagggacaag ccatggaggc
    10321 gttgtgcttc cggacggaac gctcgcaaag gtgaagatag actttgatac cctcaaaacc
    10381 ctctctgaaa ttgcgagaaa ggaatacgga ctgtccggtg cggtgcagca cggtgcctcc
    10441 acactcccgg aagaggcgtt cgacatgttc cccaaaacag ggacatctga agtgcatctg
    10501 gcaacaggct tccagaatat cgtgtatgaa agcaagcact tacctgagga attcagggaa
    10561 gaggtctatg cattcataaa acaggaatac tccaaggaat ggaaagaggg tcagacagag
    10621 gagcagttca tctacagcac gaggaaaaag gggttcggtc cattaaagag caaatggtgg
    10681 cagctccccg gagaggtaaa ggaacccatc atgaaggcgt tggaggcgca gtttggcctg
    10741 ctcttcgaga agttgaaggt tacccggacg atggacattg tgaagaagac ggtgaaaccg
    10801 gcaatagtag caaaggaaat caatcctaga ctgcaggact aggcaatgga aaaagatcat
    10861 ccaaggaggg atactcatgc ctgacatcaa gagaatcggg gttatcacca gtgggggaga
    10921 cgcgggaggt ctcaatgccg taatcaaggg cgttgcgcgg gaggcttacg cacacgggat
    10981 tgagtctgtg gttatcccca acggctacgc aggactctat aacctgattg acttacagaa
    11041 tgtggtggtg ctcaatgctg aacgggtgag ccgtatcgag gcatccctgt caggttcaga
    11101 ggcaggacac tcacgggtga agatcagcaa gatctcagat ccccaacgat atgaacgcat
    11161 taaggaaggt ctcaagaaat tcggcataga cgcactcatc ataagcggag gtgatgatac
    11221 cggaagcgtg gtggtagatc ttggtgcgca gggcattccc tgcgtccacg tccccaagac
    11281 tatggacctt gatctccagc cttatagcgt gggaggtgat tctgccataa acaggattgc
    11341 ggttatggtg cgggacctta agaccaccgg catgagccac aatcgcgtca tggtggttga
    11401 ggtgttcggc agatatgtgg gacatactgc cttacgcgga ggcatcggcg cagaggcaga
    11461 ctgtatcctg atacccgaag tccctgttga ttttgatgtc gtgtacgagc atatcaaggc
    11521 cacctatttc gcgaggattg agaggagcga cgtgaaggcc gggacctata tgatggttgt
    11581 tgctgagggg atcaagactg ccagcggcga attactctat gatgaatctg ccggtgtgga
    11641 cgcattcggt cacaagaagc tcgcaggtgc cggaagatat gtgaggcagc agattgagaa
    11701 gagactcaag gcagaccctg aagtgaaaga gttcatgaaa cggacaggca tgtatgctcc
    11761 cggcgtgtat gagatacctg aagtgagaga agtggcgccg agccatctcg tccgctcagg
    11821 acagacttca gcatatgacg tcaacttcgg ataccggaca ggtgctgcag cggtgttgct
    11881 tttacttgaa gggaaaacag gtaatacggt ggtagacgtg aacggtaata agatatcgta
    11941 catgaccact tccgaggcga taaaaaggag agaggtggat ttgagcgaga tcgcactctt
    12001 cgaaaccctc ggtatctgtt tcggcagaaa gccgcagaaa tttgaatacg agaccatggc
    12061 cgcaaagggg actgtagagc gccatctcta agatctgccg aaagggacag tcatatcagg
    12121 caatgagcaa gaaactcggg aataagatcg cgattattgt cggcggtggt cctgcaccgg
    12181 ggattaatgg tgtcataagc gctgccacca ttgaagccat tgccctgggg atgacagtga
    12241 tcggcatcag cggggggttt aagagtctct ttgacggaaa ccgggactgc gtgattcctc
    12301 tcaccattga tgaggtttcg aggatacata cttatggggg atgcatcctg cgaacctccc
    12361 gtgaatatcc cgaaatggtt aaggaaaaat tccaggttct catgtcaaca ctcaaaagga
    12421 tcggcgcaac ccatcttctc acggtgggcg gagaaggtac cctctttatg gcgaactgga
    12481 tagaaagaga gtcgagagga tctatcagtg tggtccatgt cccaaaaacc atagataatg
    12541 atattcccct gcccggcggg gtttctacct tcgggtatca gacggcgcgc caccggggag
    12601 ttgagattgt ggagaacatc atggaagatg cgaagaccac gggaaggtgg tatttcataa
    12661 cgaccatggg caggcacaca ggccatctcg cgctgggtat tgggaaggca tcaggtgcaa
    12721 ccgtaagtct catccccgag gagtttgagg gagaaacgct ctcattcaag aaggttgcgg
    12781 acatccttac ctgctccatc attaaaaggc tctcgatgca taaggaccat ggggtggcaa
    12841 tcctgggaga gggaatcgcg gagaagtttg atatggatga actcacccat tatgagcagc
    12901 tggaaagcga cgagacggga agagtacgcc tttccgagat tcagctggga agagtaatga
    12961 agaattttgt gaagaagacc ctggaatcca tgggcataag aatcaccatt gtggacaaga
    13021 atatcgggta tgaattgagg gcagcgaacc cgattcccta tgacgtggaa tatacgaggg
    13081 atctcggtta cggggcggtg aactacctcc acaagggtgg cacgggagcg atgatcgtcg
    13141 tgtatgaggg aagactcgtc cctgtcccct ttgtagagtt gattgattat tctacgggga
    13201 aggtaaagat acggatggtg gatattacct ccgagaccta caccgtggca agaaagtata
    13261 tgattcgtct cgagagggat gacttttctc cacagaatct caaaaagctt gcaaagtcag
    13321 ccaacctgaa cacttccgaa ttcagggacc gttttggtta tctcgtaggg cctcctgaga
    13381 tgtagtaatc ctcacattcg tcactccctc ctttatatgc tacaatagca ttatatgcca
    13441 gcattaaata ttggttgtag cggcttcctc tacgatcact ggaaaggggc cttctatccc
    13501 gaagacctgc cgaagagacg gtggttcgaa tactactgca caaaattttc taccgttgaa
    13561 ctcaatgtga ccttctacag acttcccgat aaggagacgt tcaccaaatg gtataaggag
    13621 acccccccgg gttttgtctt ttcccttaaa gggagcaagt ttatcaccca tatcaagaaa
    13681 ttgaaggcag cggccgaacc tgtggatgtt tttttctcca gggcattggc attaaaggag
    13741 aaactcggcg gagtgctctg gcaattacct cctggattaa aggctgaccc aggtcggctt
    13801 gcagaatttc ttgaactcct taagccttac ggggtgagaa atacttttga attcagggaa
    13861 ggaacgtggg tttcaaaaaa ggtgtttacc ttgctggaaa agaggaatgc atgcctctgt
    13921 atggctgatt ggcccgagtt tctggatgac ctccccctta ccgctgactt tacctatgtg
    13981 agaagacacg gcaaagaagg aagttatgcg acatgttaca gtgctgagga gcttaaggct
    14041 gatgccacac gaattcggag atatctgagg cagcgcaagg atgtttatgt atattttaac
    14101 aacgatgccc ttgggtatgc gccgaaaaat gcgtctgaac tgattaccat gatgaaaaaa
    14161 taaaagaagg agccaagggt cagaggggtc aagggaaaac caatacgata tgtttggcta
    14221 tttcacatga tcactcgaac ctctgttcct ttgtagcctc ctcatgagaa cctatgatca
    14281 accaacaaat cgccaggata tttaacgaaa tggctgattt gttagagatc aagggagaaa
    14341 accctttcag gatcagggcc tacaggcgcg ctgcccagaa cgtcgaaggt cttcccaagg
    14401 acgttgcgca gatgacggaa gatgaaatcc tcgagattcc gggaattgga aaagaccttg
    14461 cgggaaagat acggcattat gcagattcgg ggaggatgga ggcatacgaa gaactaaaaa
    14521 gtctggtgcc tggaggcctt gccgcgctca tctccgttcc tggcctcggc ccgaaaaccg
    14581 cgaagctcct ttacgaccgt ctcaagataa agggcatcga cgatcttgag cggctcgcac
    14641 gggcacatcg actttcggga cttcccggca taaaggacaa gacagaagag aatatcctca
    14701 agggcattga gatgatgaga aggggcaaag agagactacc catcggaagg gtgctcccca
    14761 tcgccaatga gatcatcgcc tggttgaggg aaaaggcccc tgtacaagag cttgtccttg
    14821 caggaagcct caggagatgg aaggatacca taaaggatat cgacattctc gccacctcag
    14881 gaacacccgg gaaggttatg ggtctcttcg ttcatctccc catggtgaag gagatcctga
    14941 tgcgtggtcc cacaaaatcc agtgttctga ccaccgaggg gatacaggtt gatctcaggg
    15001 ttgtggataa ggattccttt ggtgctgccc tcgcatactt tacggggagc aaggcccaca
    15061 acataaggat cagggaaatg gcagtgaagg ccggactgaa gatcaatgag tacgggatat
    15121 tcagagaaaa agacagtgca cggctcgggg gaaaggatga aaaagacgtg taccgcctcc
    15181 tcggcctccc ctctattcct cctgaattgc gggaagacac gggtgagatt gaggctgcga
    15241 tggagggaag gctacccaaa cttctggaac tcagcgatat aagaggagat ctccatgtgc
    15301 atacgaaacg gagtgacggc agccacacgc tggaggaact cgtcgacgcc gcaaggagca
    15361 gagggtatca gtatcttgcc gtcacagacc acacaaaggg tcttggcatt gcgaggggat
    15421 tgaacgaaga caagattatg gagcagatga aggagatcaa gaaagtaaac agtcggatga
    15481 ggggattcag ggtgttcatg ggtgctgaag tggacatcag aagtgattct acccttgacc
    15541 tccctgacga aatcctcagg cagcttgata ttgttgtggc atccatccat tcggggttta
    15601 aacagagcag ggaacagctc accaggcgac tcgtagccgc aattcagaac ccttacgtgt
    15661 cggtcattgc ccaccctaca gggaggctta taggggaaag ggatgcctat gaggttgata
    15721 tggagcgggt gttcgccgca ctcaaggaat cgggtaaggc cgtcgagatg aatgcatacc
    15781 cgctcaggct tgacattaac gatacccttg cgaagaaggc gaaggaaatg aacattcctg
    15841 tcgcgataag caccgacact catgtcttaa gtcaattcga ttatatggtg tatggagttt
    15901 ccatagggag aaggggctgg ctggagaaaa atgatgtttt gaatacactt cacaccggaa
    15961 atctgttaaa atggttacaa agatcgaaac tgtcacacct tagagctgag aggtgaaaca
    16021 atgattttgg tatacgaaaa agaccaaggg acactaaaat cactccgctc cttctttaag
    16081 aagaggaaat acctcaaggc cgagttcata gaggatatga cctcgctggc ggcgaggacc
    16141 tcaaggatca agggaaaaaa tctcatctgt atcattgctg caggcgaact gccgaaactc
    16201 aaaccccggg aactgaactg ttcgttcatc gcaacagttt caggagcttc tcgaggggtg
    16261 ataagaaaag cactggacta tggtgcggaa agctacctta tcactcctct ccatgaagaa
    16321 gaccttgagc agaagatcag ggatgttata aaagaaaatc aggaaaggca acgtatgcgt
    16381 aaagagacgc atatgctgca gaccatcatt gatatctctg gtcacatatc atccaccctt
    16441 gatccgaaag agatcctcta tctcatcgtg agcaatatat cggagatcat tccggtaacg
    16501 agatgttcca tcatcagtgt ggatggcggc cagcggtacg cccatgtgat ctccaccttc
    16561 gaagacccgc atctcaccaa cataaagctg gaccttaata aatatcccga aatacgggag
    16621 gcactctcgt caaaaaaacc cgttgcgatc aagaacgcag ccaaggaccc cattatgaag
    16681 agggtgagcg agatcctctt tcccctcggt attcgctcta tccttgtcat ccccattatt
    16741 tttcatgaag agattatcgg aactctcttc ctgaggacct ccagggcacg ccacgcattc
    16801 acaagacatg agataaacct ttgccaggcc atcgccaatg cctccgcgaa ccccctttac
    16861 aacgcctatc tgttcgaaaa aatgggggat gaaaagtcac accttgagaa gcttgccgtc
    16921 accgattatc tcacgggatt atacaacaac aggtattttt actaccgcct cgccgaggag
    16981 tacagccgct cagtcaggca tagctttcat ctcagctgcc tgatgcttga tatcgacttt
    17041 ttcaaggaag tgaatgaccg gtatggccac aaggcggggg acaatatcct taaagaattg
    17101 gctcaactcc ttaagcacca tatgagaaaa agcgatgtcg tggcgagata tggcggcgaa
    17161 gagttcatta tacttcttcc ccagacagac gcaaacggcg ccctggccaa ggcagagagc
    17221 ttgagaagcc atatagagaa ccacccgttc cgcggactta agggggaaaa agggctgtcg
    17281 gtgagcatcg ggttggcaac ccaccctgac catagggccc ccaataaaga agacctcatt
    17341 actttggctg acaatgccct ttataccgca aaggccatgg ggagaaacca ggtagtgttc
    17401 tctaaaccat gagttgaaaa cctcgtgcgc ggggaatacc tttattccca atgttaagtt
    17461 atgcgcgagg ttgttgtctc tcgtacaatg gatataatca cttcccatac caatgcggac
    17521 ttcgacgcct ttgcctccat ggtggcggca agaaaactct atccgggtgc actgctcgtc
    17581 tttcctggct cacaggagaa aaaggtgagg gacttcattg ccgcattcca gcccgttgag
    17641 atatcccgcg tgaaagaaat agagattgca caagtgagac gacttatcct cgttgataca
    17701 aagtcctctg agagaattgg tccgtttgcg gaattggctg cgaaatccgc tcaaacagaa
    17761 attcaccttt acgaccatca tcccagaaca aaggacgata tccgtggttc catggaagtg
    17821 atcgaggatg taggcgccac cgccaccatc ctcaccgaga tactgaaatc ccgtaagatc
    17881 cccataaccc ccatggaagc aacgatgctc tgccttggga tctatgaaga gacgggctcc
    17941 cttctcttcc cctccaccac agagcgtgat atgattgccg cagcatacct cctcagaaga
    18001 ggagcaagtc ttaagattgt gtctaatttt attcggacag aactgagcaa ggaagaactg
    18061 gatattctga atgaactctt aaggtcatcc acccatctgg agatgaatgg caccagggta
    18121 atgatcgcga aggcatccag ggaagactac ctcggggatg cggcccacct tgcccacaga
    18181 ataatggata tggaggacac ggatggtctt gtgctcatcc tcagcatgca ggggaagata
    18241 ctgatggtgg gaaggagcag ggtgccggaa tttgacgttg cggagataat gaagaagttc
    18301 ggcggcggcg ggcatcctaa cgcagcatcc gccacactta aggagacacc tctggaagtg
    18361 ctagaggacg aggtgaaaag cctgctcaaa tcatccgtac gctatggcaa gagcgcgaaa
    18421 gacgtgatga ctaccccggt aatcaccatc tcatggaccg atgcggtaaa agacgcggaa
    18481 tctatgatga cgaggtacgg ggtgaatgtc ctcccggtgg tgatggacgg aaaatacaat
    18541 ggaatcattt cgagggaaat cgtggaaaag gccatattcc acggctttgg cagaagcaga
    18601 atttctgatt tcaccactac cgatgcccag acggtgagcc atgataccca agtgagggat
    18661 atagagcggc ttatgataga gcagaatcag cgcttcatgc ccgtgctgga aggagagcgg
    18721 atcatcggtg ccatcacaag gacggatatc ttgaggatac tttatgaaga tatgctgaaa
    18781 cgcagcaggg tacagtctcc cgcgggagaa aaaccatcaa tgggacgaaa cctggccccc
    18841 tggctcaagg aaaagtttcc tcccgcaata gcggagatcc tcaaactctc gggtgaagtg
    18901 gcagagagcc tcgggagtaa agcttatctt gtgggcggct cggtaaggga cctgctacgg
    18961 ggacaggaga atctggacat tgatttggtg attgaaggag agggaatacc atttgcgagg
    19021 gaagtggcaa agagacttgg cgcaaaggta aggacacatg agagatttgg aacagcccag
    19081 gtaattaccg accatctcaa actcgatgtg gctaccgcga gaaccgagta ctacgagtcc
    19141 cctgcagcac ttcccaaggt ggagacctct tccataaaga aggacctcta ccggagggat
    19201 ttcaccatca acacccttgc catttcgctc aatcccagag attttggcct cctcatagat
    19261 ttttttggag gtcaaaggga cctgagagag aagaccatac gggtcctcca taacctgagt
    19321 tttattgaag atcctacaag ggcattccgg gcagttcgtt tttcggaaag gtttggattt
    19381 aaactcagca aacacacgga aaatcttatc aagtccgcac ttagaatgaa tctctttgac
    19441 aggctttcag ggtcaagatt atacgaggaa cttatcctta ccttcagcga aacggaaccg
    19501 gtgaaagcca tcaaaaggct ctcccaatac ggcctcctta aggtgattca ccagaacctt
    19561 gcctttgacg aaagactgga ctccatgctc cagtccatac acgataccct ctcgtggttc
    19621 aatttgcttt ttttggaaga gaagcctgac aaaggtatca tctacatgat ggcactgttg
    19681 tcggttcttg gcgatggtga caggggaaac gcttttataa ggctatccac acctcccagg
    19741 gttaaggaaa gaatcaataa aggcatcctc cattcacagg aactggtgaa actcttaccc
    19801 ctccatgacg ctgcaaaggt ctataaagca ttgtatccgc ttgacttaga gagcgtcctc
    19861 ttttcaatgg caaccgctgc gaatgacaat aagaaaagag agctctcgca ttacctcatt
    19921 gagctgagaa gggtgaaacc ccagttaaca ggtcaggacc ttaagaacat gggtatcatg
    19981 ccaggccctg tctactccga aattctggat acccttttgg aagagagatt aaggagaaat
    20041 ctcaaaacaa gagaagacga attggcattt gtgaaagaac gctacaagac aatctcccat
    20101 tattgaatga aaaagacatc actgttactt tgaaatattc ctgaaaagag tctataattt
    20161 taataaagct cataaaaagg aggctctatg gcgcgggtat atctgctggt aaatgtattg
    20221 ccgggtaagg atgtcttcat aagagatacc ctcaggggaa tacgtggtgt ggtgagtgct
    20281 gacgtggtca cggggcatta tgatattgct gccgtaatag aggggaagga tacgggagag
    20341 atatttgaca agatctttca gaagataaga aagatcaagg ggataaaccg tacggaaagt
    20401 tttgtggcag tggaatagta tgtgggggtg gcctaggatg gaaggctctg gtgatgaata
    20461 actgggcgag cccttggtat ttcttaggtt aagcgatgca gttctacctc actgcatcgc
    20521 ttctttcagc cgcgcaaggt tatacttcat gaggccaaga taggtctccc ggccaggaag
    20581 cccgccttca gggtcaagca taataacctt cactccggcc tcgtgcgcta tcacctcagc
    20641 ggtcttcggg ttaagctgtg gttccgcaaa gacagctctg atatggtaat gcctgatagc
    20701 cttcacaatc gcctgcagtc tttggggtga aggctccttg cccggtgatt cttctattac
    20761 cccaacactc ttaagcccgt agcggagggc aaaatagtcc cacgcagggt gaaagcttac
    20821 gaaactcttt atggtgaagg tcgaaacgtt ctttctgatc tctgcatcaa gcgcctggag
    20881 cttcgcaata tactcagcgc tccgcacgtg gtacgtgtaa ccaccctggg gatcagtctc
    20941 ttggagggtc ttttcaatct tcctgaccat ctccatagcg agcaaggggt caagccagac
    21001 atgagggttc gccgtttcat gcccgtggcc gtggtcgtgg tcttcctctt cgtgtaaaag
    21061 cttcatcccc tctgaaagga gaatgatcct gatggtttcc ctggaagctc ttacgagttt
    21121 ttcagcccag aaatcaagcc ctgcgccaac cccgaaaaaa agccttgcct gggaaatatc
    21181 ctttgctgta cccgttgtgg gctcaaagac atgggggctt gcccctggcg ggagcagtac
    21241 tcgcaccgct acccggtccc cacccacctc tctcaccatg tccccgatgg gagagatact
    21301 tgccacaaca tttaattgtt gagcgagggc tcctgaggca gagagggtaa aggcaaggaa
    21361 ggatatgaga aggggtgcca gccatttcat gcaacgacca cccatggaga gcgcgttcaa
    21421 aaggatatgc acctctctgt ccttcatagt gaatgaatta actttatcag aaaaaagggg
    21481 gcctgtgaag ggcccacacg ttgggtccat ctctgataag tctgttgata tcgtggcttg
    21541 aggaggactg cactagatag agcgtcttct catgccggga taaggacaag aacagaattg
    21601 acgtatctct gccttccgtt ctatcataat ccgtcatggt aaagcctgaa aagcagttag
    21661 agattatcaa aagggggagc gtcgagataa tactcgaaaa ggatctcttc cttaaactgg
    21721 aaagatctta caaagacaat tctcccttgc ggattaaggc tggctttgac cccactgccc
    21781 ctgatatcca tcttggccac accgttcttc ttgagaagat gaggcagttt caggaactcg
    21841 gacatgaggt aattttcctg atcggtgatt ttaccggaat gatcggtgac ccctccggaa
    21901 agagcgaaac ccgtaaaccg cttactaaag aagaagtgtt gaggaatgca gagacgtata
    21961 aaacacaggt attcaagatt ctcgaccagg ggaaaacccg gatgctattc aacagtgaat
    22021 ggctgggtaa gatggagagt atggaactgg tcaggctagg tgccatgcag accgttgcaa
    22081 ggatgcttga acgggaagat ttcaagaagc ggttcaagaa ccagcaggac atcagcatat
    22141 tggagtttta ctatcctctc tttcaggcct atgattcggt ccaccttaag gctgacgttg
    22201 agctgggcgg cactgaccag aagttcaatc tattaatggg caggacgctg cagaggaaga
    22261 tgagcattga agaacaggtt gttattatga tgcccctgct tgaaggaact gacggggtga
    22321 ataagatgtc caagagcctc ggtaactatg tcggcattca tgaacctgcc tttgaattcg
    22381 tacacggtga agcgaccggc atgtttccca agatcatgcg tatacccgat aaattgatta
    22441 tccgctatct cgaactcctc agccacatct caatgaatga gctctccgct ctcaggcagc
    22501 gggagcagga aggctctctg gactacctga aggcaaagag tgatttagcc atggagattg
    22561 tggccagata tcacggaaaa caggaagcag aacgggcaag ggaggaattc gaacatgtca
    22621 gggacaagaa acttcccgat gatatcccga caataactat tactctcgag ggaggaagtg
    22681 ttgcgggcgg tacactgaaa gttgaattga aaacgaggaa gacctggctg cctcatataa
    22741 tgaaaactac aggactttcg aagagtacga gcgaggcgat aaggcttatc caacagggcg
    22801 gcgtgaagat aaatgaccat actattaccg actccaacgc tgaattgcac aaaggcgaac
    22861 acataataaa ggtgggtaaa cgaaaattct ataaggtgaa gatacagtca tgatatctaa
    22921 tgccgaaata atcctcagac tcctcatcgg tgcggtattc ggcggcattg tcggtttcga
    22981 aaggcatgcc catggaaggg cagcgggctt cagaacccat cttcttgtct gcgtggcttc
    23041 tgtcctggcc atgatcgtct cggagtacta ccactacctg agcctgtttg acccgtccta
    23101 tgtgcgagcc gatcccggac gtatcgctgc cggagcgatc accggcatgg gctttatcgg
    23161 agcaggggta atcgtcaaaa tgggctttaa tgtccagggg ctcaccaccg ccgcgtgcct
    23221 ctggattgtt tccatcgtcg gtctctccat tgggagcggt ctctacgtgg cgggaacgac
    23281 agggtttgtg atcacctacc tggcgctttg gttgctgaga cagtgggaag agaggatgcc
    23341 tgggctctca tataaatttg ttaacgttgt catgcaggga atgggagatg aagagtccat
    23401 caatttggcc atcaagcgat gtggagcact cattgaaaag acagactacg agagggatct
    23461 ggaacataag gaggccacgt ataacatcac catcactttt aagggcgagg gctgtacgga
    23521 aaaactcctc gccgaactct cttctcttca gaatgtgaaa agggtcatga ttcgcagtta
    23581 acgttccctt tcgccttagc ccgtataatc tcagagacca aagcctctat agttgactcc
    23641 tttggcatga tatccacctt cagacctgcc ttctctatcg cctttgccgt caccggccct
    23701 atcacggcga tggtaacccc cttaaggagt tcatgggcat cgctgcccat tatttcaacg
    23761 aaatttttga acgtagctgc gctggtgaag gtcgcgatgg tgacgcggcc ctccttcagg
    23821 aacctcttca gcctttttcc gtgaagctcg ggttttaccg ccctgtatgc aacagggaca
    23881 tcaatctcac cccccaactc ccgtaccttt tccgggaaaa tctcccttgc aatttccgcc
    23941 cttggcaaca ggaatttcat gccttttaaa agttcagggc caagactttt ggcttttgac
    24001 ttttggctct tgacttcctt tacaaatgcc tcgatcaaac cctcagcgtg aaattcatcc
    24061 ggcacgagat caacttttat gccatattta tttatctcgg atgcggtctt tgcccctatg
    24121 gcacagattc ctatcccctt aaggtccctg atatccctgt ctttctccac aaatctgttc
    24181 agaaaatacc tcaccccatt ggcactggtg aatataatcc attggtaaga ctggatattc
    24241 ttaatggcag catccatctc cttgtagctg agcggcggca caatctcgat ggtgggaaat
    24301 tccacaatct ctgcaccaag ctcctcaagg ggttcgaatc ccgacgagtg ctctctcgtg
    24361 acgagcaccc tgtggccgaa catgggcttg tcttcatacc atctgaggga gtccctgagt
    24421 ctcaccacct cgccgatgac acacaccgca gggggtttaa tctctttatc cttgatctcg
    24481 gaaacaatat cccttagtgt gctcactatg gtcctctggt cagcccttgt accccaccgg
    24541 accactgcca caggggtccc ggggtccttt ccgttctcta tgagtttctg tacaagcaga
    24601 tccaggttct tcaccgccat gagcaccaca agggtgccta ctcctgtaga gagtttcgcc
    24661 caatcaatcg aagaaccttg ctttgtggta tcttcgtacc ccgggatcac tgcgaaggag
    24721 gaggaataga gcctgtgggt caagggtatc cctgcatatg ccggtgcagc aacggcagaa
    24781 ctcactccag gaaccacctc gaaggtgata ccgttcctcg caagaacctc tgcctcctcg
    24841 ccgccgcgtc cgaagataaa ggggtcaccc cccttgagcc tgcagataag ttttccctcc
    24901 cttgcctttt ccacaaggag ttcattgatc tcatcctgct tcagggtatg gtgtcctcca
    24961 cgctttcccg catagataaa ttcggcgtca tggttgatgt agttcagcac ctgcgcattg
    25021 agatggaaat catacaccac cacttctgcc ttctgaaggc acttcattcc ctttaccgtg
    25081 aggagcccta tatcaccagg cccagccccg acaagatata ccttcccctt atctttccgc
    25141 atgcggttaa ttgtatcata gaaagactcc acagaggtaa tgtactgagg cgtgtccccc
    25201 tgctgtgcta tactttgggt ggacatcatt aagaagaaac ggggagtgtt atcgaagagg
    25261 tgattcagta agagaacttt cgggtgcgga tatgacgcat gctttttagc cacaccctga
    25321 actctcttgt catcaaatcc catgactata cgtgaacaga ctgaagagat agaaaggaaa
    25381 accctccatc ccagggcatg cctgagttcg ggaagcaagg gaagggtgac cccggaaaaa
    25441 gaaggggaga taaggacgtg ctttcagagg gacagggaca ggataatcca ttcgaaggca
    25501 ttcaggaggc tcaagcataa gacacaggta ttccttgcgc ccaggggcga tcactacagg
    25561 acaaggctca cccatgtcct tgaggtatcc cagatagcgc gaaccattgc cagggcactg
    25621 cggttgaatg aggacctcac cgaggcaatc gcattgggac atgacctcgg ccacaccccc
    25681 ttcggacatg caggcgagtc tgtcctcaga gagatatatc cgggagggtt cgaccactat
    25741 aagcagagcc tcagggttgt ggacctgctc gaaagaaacg gccagggcct caaccttacc
    25801 catgaagtga gaaatggcat tgtgacccac tccaagggca aaggactgat aattcctgaa
    25861 aaacgcagcg agagggcaga aacgctggaa ggccttgccg tgaggatatc cgatgtc
//
LOCUS       DATKOU010000038        14462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2742966, whole genome shotgun sequence.
ACCESSION   DATKOU010000038 DATKOU010000000
VERSION     DATKOU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 14462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14462
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742966"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VMU21_01605"
     CDS             <1..756
                     /locus_tag="VMU21_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUO76248.1"
                     /translation="TEPSCGSDAKAIQTRAVHSGDSYILNGTKILITNPGEAGVILLL
                     AATGKGISAFLVPKEAQGFHVMKDIPKLGLKGSRLSAIQLKDCRVERSTLIGEEGKGL
                     EYAKQILNEGRLTIGAIAVGIAQAAHEKSLLYSKKRTAFNQSISSFQLVQEKIANMAT
                     EITASRLLVYSVAMLREQGKSTAYEASQAKLFSTEMALRVCDNALQIHGGYGYVDAYD
                     VHRHWRDARGLTIGEGTSDMLRLLIAHLALKRA"
     gene            770..1543
                     /locus_tag="VMU21_01610"
     CDS             770..1543
                     /locus_tag="VMU21_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUO76249.1"
                     /translation="MNTTDKIRVDKKKDIGIITFNKPQVMNVMGTDMLMKLEDALTDL
                     ENDGQIRAVIMRGTTHFSAGADIKELKGKDPETAESFSRLGHRICNQIEDMGKPVIAA
                     VEGYALGAGCEIALACDIRIAAENAKFGQPEINLGLIPGFGATQRLARLIGPGRAKEM
                     ILTGRFVDAKEAATIGLVNKMVKNEDLMAKTEEMGQTMATKSPVAMKMAKTLLRGDHE
                     MQRLLDLEITYLSQCFATEDHLEGITAFLEKRTPRFKSI"
     gene            1572..2936
                     /gene="nhaA"
                     /locus_tag="VMU21_01615"
     CDS             1572..2936
                     /gene="nhaA"
                     /locus_tag="VMU21_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HUO76250.1"
                     /translation="MKKFFTDYLTSADRLKAGLIKPFHLFFRAEASSSILLICATAVA
                     LLWANSPFGAAYHHFWESELSVALGGYSVVKSLREWINEGLMAIFFFIVGIEIKREVS
                     TGELASLKKALLPVGAALGGMVFPALVYFAFNHGTPTVHGWGIAMATDIAFALGALFI
                     LGKKIPFGLRVFLSALAIADDLGAVVVIALFYTETIALSSLALGLLAVILMVIANLLW
                     VRRTLVYALLGVGLWFALLGSGIHATVAGVLVAMCIPARGKYDTDRFLGEVSKYLGEF
                     HCPPDGCGHSILLNEKHRDAVQSIELACHDVETPLQRLEHSLHPWVAYLVIPVFALAN
                     AGLTLGTIDVAQALTSPVTLGVGLGLLFGKPIGIVLFSYLFTRKNLSSLPAGVAWPHI
                     IGAGILGGIGFTMSLFITSLSFRDPLMVDFAKVGILIASFVSGIVGIAFLHVYSVRRE
                     EEKD"
     regulatory      3073..3214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            3480..4322
                     /locus_tag="VMU21_01620"
     CDS             3480..4322
                     /locus_tag="VMU21_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76251.1"
                     /translation="MKRLRIIVVLTVLAVFLSGGIALGEDQKPAEQKAEEPKVTGSAS
                     AAFLSQYIFRGYEIGSHSMVIQPAITLSYYGFSANLWSNFDTRQHPTQSFFPSTTGNS
                     SLNETDLTLSYTYTIDKLSLTGGYIWYGTQYANQTQEFFLSAAYDIISKPVLTIYQDI
                     DNYPGTYFQLAFSHSLPVYKEITFDMGASFSYEWGQGNYWKTYQSSTGAYTGDKYSAF
                     HAGMLQGGVTIPVTKTFSVQPVLQWWFPLSGDAHKTVNGVSYNPNGKLDNTVVFGLNM
                     AFNF"
     gene            4768..6564
                     /gene="kdpA"
                     /locus_tag="VMU21_01625"
     CDS             4768..6564
                     /gene="kdpA"
                     /locus_tag="VMU21_01625"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HUO76252.1"
                     /translation="MQYTGMTFDIVQILALLVVLTLLVKPFGGFMKRVYEGEKNLLSP
                     VLGPLERVVYRFSGIRPDDEMSWKQYASAMLIFNGLGLVVLLSILMLQGYLPLNPSKF
                     PGFTWHLAINTAVSFVTNTNWQAYSGESAASYLTQAAGLTVQNFVSAATGMAIVIAFI
                     RGLARRTAETIGNFWVDMTRSVLYILLPISLVAALVLVSQGVIQNFSPYKTAQLVEAT
                     SYEKPKIDAEGNPLKDEKGQAVTEKVVVKEQTLPMGPVASQEAIKELGTNGGGFFNAN
                     SAHPYENPTPLSNFLEILLILSIPAALTNTFGRMVGKTRQGWAIYTAMMFLLVMAVGA
                     LYWAEYSGNPLLQKLGVPGINMEGKEVRFGTGWSALFASATTGTSCGAVNTMHDSLTP
                     LGGMVPLMLILLSEVVFGGVGSGLYTMLAFVIIAVFTAGLMIGRIPEYLGKKIEVTEM
                     WMSVITVLTAGVVILLFVAIALITPSAVSSMANPGPHGLSEVLYAFASMSNNNGSAFA
                     GLNANTVFYNLMGSLAMLAGRFGPAIAILAMAGSLVKKKYVPASVGTLPTDQLPFIFW
                     LVSVVLIVGALTFFPALALGPIVEHMIMQGGV"
     gene            6567..8633
                     /gene="kdpB"
                     /locus_tag="VMU21_01630"
     CDS             6567..8633
                     /gene="kdpB"
                     /locus_tag="VMU21_01630"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HUO76253.1"
                     /translation="MAKHIEKSKGIFDPKLMKAALIDSFKKLDPRALWRNPVMLSVEI
                     GSVITTVNFIYNLVTGKGEPAWFTGIVSAWLWLTVIFATFAESLAEGRGKARAESMRK
                     TRKEIMAKKLKKPELGGEYELVPSTQLRKGDIILVEAKDMIAGDGEVIAGAALVNESA
                     VTGESAPVVRESGGDRSAVTGGTQVIANSIVVRITANPGETFLDRMISLVEGAKRRKT
                     PNEIALEVLLVAMTVVFFLVVVNLSPLSIYSVKAVGQGQPVTLVALVALFVCLIPTTI
                     AALLPAIGIAGMDRLFQKNVIALSGRAIEAAGDVNVLLLDKTGTITLGNRQAVAFIPV
                     GGHSEKELAEAALMASLTDETPEGRSIVVLAKQKYELRMRELPHNAESVPFSAETRLS
                     GVNADGHAYRKGAGDSIVRVVQGMGGTIPHDLENHVTDIAKSGGTPLVVSRDTEVLGV
                     INLKDILKGGIQERFLQLRRMGIKTVMITGDNPLTAAAIATEAQVDDFLAQAKPEDKL
                     RLIRENQQQGYMVAMTGDGTNDAPALAQADVAVAMNTGTQPAREAANIIDLDSNPTKL
                     LDIVEIGKQILMTRGTLTTFSISNDVAKYFAIIPAAFAAVYPQLGILNVMHLANPFSA
                     ILSAVIFNAIIIPLLIPLALKGTPYRPMPAERLLVYNLLIYGVGGLLAPFAGIKLIDV
                     LINVLI"
     gene            8647..9210
                     /gene="kdpC"
                     /locus_tag="VMU21_01635"
     CDS             8647..9210
                     /gene="kdpC"
                     /locus_tag="VMU21_01635"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HUO76254.1"
                     /translation="MKELKNGILLFVALSLLTGVLYPAAVTVLAQLMFPQQANGSIMY
                     TADGMPIGSSLIGQPFSDPKYFWPRPSATTDFPYNALASGGSNLGPTNKDLISQVADR
                     VKSFRESGIQGQLPADLVTASGSGLDPDISPEAALIQIPRIAKERNVSEEKLRAMVLA
                     HLEDRQFGFLGTPRVNVLKLNLALNKE"
     gene            9214..11148
                     /locus_tag="VMU21_01640"
     CDS             9214..11148
                     /locus_tag="VMU21_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HUO76255.1"
                     /translation="MAEDEEYKRPSPEALLEMAQKEEAQEKRGKLTIYFGAAPGVGKT
                     YAMLLDARQRKEEGVDVVVGYVETHGRSETDALLEGLEILPPLVINYMGVQLREVDLA
                     KVFVRKPKLVLVDELAHTDAPNLRNAKRYQDIEEILNAGVDVYTTMNVQHVESLNDIV
                     HQITNIRVRETVPDTVIEAADEIKLVDLPPEELLKRLHEGKVYVKDMAGSAVQKFFRP
                     GNLLALRQLALRAVAGSVDEKMRTYMRAHAIAGPWSATDRVLAGVFASPYAEKLIRAA
                     FRLANYLDAEWIAFYVETERHKTLSEKEKEWLNKAMDLAKLLGARLVWIKGTDVMEEM
                     ADYARNNNVTKIVIGKPRFGLFQSVPKKLLTRTPNIDIYLLDARVGPRHMPKKRMIFT
                     RPMNYALGIFAVGIVSLIGFLLRNVLNEVNLLFLLLLPVILSALYLGKGPSIVAAITS
                     ILIFDYLFVSPHFSFAVGDVQYFFSFVVYIIVVVVISNLASKLRGKVEMLKQSELKNI
                     GLYGLSKDLVTAHTIDQVLSLMVRHTLEIFPCEMAIFLPEEGNLTVKAKTPDFEVTPK
                     LLGVASWVMINKQPAGRGTSTLPQATAYYLPIMSGEEAIGVAGFDFRGSEGMMTSEKR
                     VVLKTITRLGAMAIERIRIQ"
     gene            11152..11826
                     /locus_tag="VMU21_01645"
     CDS             11152..11826
                     /locus_tag="VMU21_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HUO76256.1"
                     /translation="MVTANNVVSESGNDNTPFYKWANFLALITIFYNLVEGLVSVFFG
                     LEDETLALFGFGVDSFVEVISGVGIWHMIRRMRRNGNENADRFERTALKITGTSFYIL
                     TFGLLIIAAVSISEGYKPETTFWGIIVSVISILTMWLLMHYKIKIGSKFNSQALLADA
                     ACTKTCICLSVILLIASVGYEVTGIGMLDSLGALGIAILSFKEGREAFEKSRGNLACS
                     CQGQCH"
     gene            complement(11849..12550)
                     /locus_tag="VMU21_01650"
     CDS             complement(11849..12550)
                     /locus_tag="VMU21_01650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HUO76257.1"
                     /translation="MPQEKKETSVRDIFSIVAPYIDPLDTAFSMGLCHLWRRRLVSDI
                     ERGEHILDLCTGTGEVAKLIIRRIGSEGSLTCLDFCEEMLEVAKRKLNPIPANMTFVV
                     SDVREMSFPENTFDAITVAFGMRNVPDTEVALKKILSVLKPGGRFLCLELTKPTKRWF
                     LPLYKWYTFKFMPAVAAIITRSEVPYTYLPRSIDVFYPPEEFTQLMRGCGFTDVTFQS
                     MSMGVATLYRAVKGG"
     gene            complement(12552..12932)
                     /locus_tag="VMU21_01655"
     CDS             complement(12552..12932)
                     /locus_tag="VMU21_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HUO76258.1"
                     /translation="MPIPIKIVIADIALDGELFDTQCAKAITEALPIETRPDEWGDEF
                     YFGIPVEMPLDETATRKVKVGDVGYWPPGKALAIFFGPTPMSRGSEPVPASEVNLVGK
                     ITGDAKLLRKAKGSRRIRIERVSV"
     gene            complement(12932..14386)
                     /gene="bioA"
                     /locus_tag="VMU21_01660"
     CDS             complement(12932..14386)
                     /gene="bioA"
                     /locus_tag="VMU21_01660"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HUO76259.1"
                     /translation="MGTIEENRHLEETDKNYIWHPFTQMKEWLDEKPVIITEGRDCFL
                     KDIYGNWYLDGVSSLWVNIHGHRKKEIDDAIKAQLDRISHTTMLGLSNVPAIQLAERL
                     VKLVNQKGSGGQGVKGSSEIKPSNPRTIEPSNPLSLSKVFYSDNGSTAVEVALKMAFQ
                     YWIHKGVEGKRSFLSLKNGYHGDTLGAVSVGGIDIFHSTFGPLLFKAYKAPSPYCYRC
                     ELDATHPDCGFACLRKMEEILRDHSSEIAAVIIEPLVQAAGGMIVAPPGYLKGVRKLC
                     TKYRVLMIADEVATGFGRTGRVFACEHEDVVPDIMCLSKGITGGYLPLAATIANKGIY
                     SAFLGEFRDLKTFFHGHSYTGNPLGCAAALACLDIFERENTIEGLGFKIGILENFLHD
                     LAKLDHVGDVRNKGLMAGVELVKDKSTKEPYAWEEKMGWRVCHYARAKGVFLRPLGNV
                     VVIMPPLSISTENLEQLLRVIQEGITEVTTGREK"
BASE COUNT         3540 a         3613 c         3809 g         3500 t
ORIGIN      
        1 acagagccct cctgcggctc ggacgcaaag gcgatccaga ccagagcagt tcattcggga
       61 gactcttata ttctgaatgg cacgaagatc ctcatcacaa atcctggaga agcaggagta
      121 atccttttgc ttgctgcgac cgggaaaggt atctcagcgt tcctcgtgcc aaaagaggca
      181 caaggatttc atgtgatgaa ggacattccg aaacttggat taaaaggaag ccggctatcg
      241 gccatacaac tcaaggattg cagggtcgaa aggagcaccc tcattggcga agaaggaaaa
      301 gggcttgagt atgcaaaaca gatcctgaac gaaggaaggc tcaccattgg ggcaatagcg
      361 gtcggaattg cacaggctgc gcatgaaaaa tcgctcctgt acagcaagaa aagaactgcc
      421 tttaatcaaa gcatctccag tttccagttg gttcaggaaa agattgccaa catggcgacc
      481 gaaataactg cttcaaggct tctcgtctat tctgtggcga tgttgcggga acaggggaaa
      541 agcacggcat acgaggcttc tcaggcaaag ctcttctcca cagaaatggc attgagggta
      601 tgtgataacg cgcttcagat acacggaggc tatggatatg tcgatgcata tgatgtccat
      661 agacactgga gggacgcgag ggggctcacc atcggtgagg gaacgtcaga tatgctgaga
      721 ctcctcatag cccatctggc gctcaaacgg gcgtgagtaa ggagttttta tgaataccac
      781 tgacaagatc agggtagaca agaagaagga tatcgggatc attaccttta acaagccgca
      841 ggtaatgaat gtcatgggca cagatatgct tatgaagctg gaagatgcac tcactgatct
      901 cgaaaatgac ggacagataa gggccgtcat catgagaggc accacacatt tcagtgccgg
      961 ggctgatata aaagagctga agggtaaaga ccctgagact gcggaaagtt tttcaaggct
     1021 tggccacaga atctgtaacc agatagaaga tatggggaag cccgtgattg cagcagtgga
     1081 gggttatgcg ctgggagccg ggtgcgagat tgcccttgcc tgcgacatac gcatcgctgc
     1141 tgagaatgcg aaattcggtc agcccgaaat caaccttggc ctcatacccg gcttcggagc
     1201 tacccagcgc ctcgccagac tgataggccc cggaagggca aaggaaatga tcctcacagg
     1261 caggttcgtg gatgcaaaag aggcagcaac tatcggcctt gtgaataaaa tggtcaagaa
     1321 tgaagatctc atggcaaaga cagaagagat gggacaaaca atggcaacga aaagccccgt
     1381 tgctatgaag atggcaaaaa ccctgctcag aggagaccat gagatgcagc gcctcctcga
     1441 tcttgagatc acttatttat ctcaatgctt cgcaacagaa gaccatctgg aggggataac
     1501 tgccttcctc gaaaaaagaa caccaaggtt caaaagcatt taaatggcag aatgctcaaa
     1561 ggacaaaaat aatgaagaaa ttctttacgg attatctcac gagcgctgac aggctaaagg
     1621 cagggctcat caagcccttc cacctctttt tcagggcaga ggcatcgagc agcatcctcc
     1681 tcatttgcgc cacagcagtt gccctcctat gggcgaattc accatttggt gctgcctatc
     1741 atcacttctg ggaatcggaa ttgtcggttg ccctcggtgg gtattcggtc gtaaagagtc
     1801 tccgcgagtg gatcaacgag ggactcatgg cgatattctt tttcattgtg gggatcgaga
     1861 tcaaacgtga agtcagtacc ggagagcttg cctccttgaa gaaggccctc ctccccgttg
     1921 gcgctgccct aggagggatg gtttttccgg cgctcgtcta cttcgccttc aaccatggaa
     1981 cgccgaccgt ccacggatgg ggcatagcaa tggcaacgga catcgccttt gcgctgggcg
     2041 ccctctttat actcgggaag aagataccct tcggactcag ggtcttcctt tccgccctcg
     2101 ctatagcgga tgaccttggg gctgttgtcg tgattgcgct tttctatact gagacaatcg
     2161 ctctgtcctc ccttgccctc ggccttttgg ctgtcattct tatggtcatt gcgaacctcc
     2221 tctgggtcag gaggacactc gtgtacgcgc tgctcggggt cggactctgg tttgccttgc
     2281 tcgggtcagg gatccacgcc acggtcgcag gggtcctcgt cgcgatgtgc atccctgcca
     2341 gggggaaata tgacaccgac agatttctcg gggaggtcag caagtacctg ggggaattcc
     2401 actgcccccc ggacggatgc ggccactcta tccttctgaa tgagaagcac cgggatgcgg
     2461 tgcaatccat cgagcttgcc tgccatgatg tggaaacgcc tctccagcgt ctcgagcatt
     2521 ccctccatcc ctgggtcgcg tatctggtga tccccgtctt tgcgctcgcc aacgccgggc
     2581 tcacacttgg gacgattgac gttgctcagg cattgacctc gcctgtgacc cttggggtgg
     2641 gccttggcct gctcttcggc aagccgatcg ggatcgtcct cttctcctat ctcttcacga
     2701 ggaagaatct gtcatctctg cctgcgggtg tggcctggcc gcatatcatc ggcgccggta
     2761 ttctcggagg gataggcttc actatgtcgc tttttatcac ttcgctctct ttcagggacc
     2821 ctttgatggt cgatttcgca aaagtgggga tcctcatcgc ttctttcgtc tcagggattg
     2881 tcggcatagc ttttctgcac gtctattcgg tgaggcggga agaggaaaaa gattaagaaa
     2941 gagatcctca taagccgttg aatactttta tgagcatgtg aattgccaca agtaatcaat
     3001 gggttgcagc aggcttcaag aaaaaaaacc gatttctctt gacaattttt ttggtgagtg
     3061 atataaataa aaattacata gcagccgaga tccattcaaa gaatggatgc gggggaccca
     3121 agaatttggg gcgtatttcc cgggatatcc gggaataggg cacttccagg cccgagcccg
     3181 tcagctaacc tcgtaggcat ttggaagggc aaaaaaggct gaaactaaac cgccctttgc
     3241 cggcggtttt ttattttctc cctttcctgc ccctctgaga aggaggacat atgccactga
     3301 aacgaactcc catttgaatg tatcggatta tgcgctatca ttggagaaag aggtgcgcaa
     3361 cggggagttg cacacccggt ggcaggaaaa ggaaagaagg caaccttcat cactgtctaa
     3421 taaacaagaa ggaagaagag aagaaataaa gatcgctgaa attagaggag gaaattgaga
     3481 tgaagagatt aagaatcatt gtggtgttga cggttttggc agttttctta tcaggaggca
     3541 tcgcgcttgg cgaagatcaa aagcccgctg agcagaaagc ggaggagccg aaggttaccg
     3601 ggagcgcatc ggctgctttc ctcagccagt acatcttcag gggctacgag atcggctctc
     3661 atagcatggt cattcagcct gccatcactc tctcttatta tggcttcagt gcgaacctct
     3721 ggagtaattt cgacacaagg cagcatccga cgcagagctt tttccccagc actacgggaa
     3781 actcgagctt gaacgagacg gaccttacgt tgagctatac gtacaccatt gacaaattaa
     3841 gtcttaccgg aggatacatc tggtacggca cgcagtatgc gaatcagacc caggaatttt
     3901 ttctgtccgc agcttatgac ataataagca aaccggtgct caccatttac caggacattg
     3961 acaactaccc tggcacttac ttccagctcg cgttctccca ttcactgccg gtatacaagg
     4021 agatcacctt tgacatgggc gccagctttt cgtacgaatg gggccagggc aactactgga
     4081 agacctatca gagttcaacg ggcgcctata ctggggacaa gtactcggca ttccatgccg
     4141 gtatgcttca gggaggggtg accatccctg ttacgaaaac gttcagcgtg caaccggttc
     4201 tccagtggtg gttccccttg tccggcgatg cccacaagac agtcaatggt gtctcctata
     4261 accccaatgg gaaactggac aacaccgttg tctttggtct gaacatggcg ttcaacttct
     4321 aggaaataat cgcttttttg gagaagaaat accatgaaac gatttttgag tttagtgata
     4381 ctgatgactt tgtcctcgca acggtccttg ttcgtttgaa ggaaacctgg agggagagaa
     4441 aatgaaaaga acattattat tattatgctt gatcgtattg ttcggttttg cgggcaattc
     4501 attcgccact tcaccgatgc ctgaagaggt caagccaggc atgacaacca cagtgtgcat
     4561 aatccatatt acttgcgtca aagatcggga aagaggaaca tacgaagatt tggcctcgac
     4621 atttgcgttc gaggtctctg aaacgaaagg agacgtgctt gaatgggtaa cagggtatgt
     4681 caggaacaaa ttgaacgaac gaaatgtgtc cgagttcgaa gtcgattgca gatttcaaaa
     4741 atagtgagga ttttttagga ggtgactatg caatacacag gtatgacttt tgacattgtg
     4801 caaatccttg cgctgttagt agtgcttacg cttctggtaa agccttttgg cggatttatg
     4861 aagagggtat acgagggaga aaaaaatctt ctcagcccgg ttcttggtcc gctcgaaagg
     4921 gtcgtttaca ggttctcagg catcagacct gatgatgaaa tgagctggaa gcaatatgct
     4981 tcggcgatgc tcatctttaa tgggttaggt cttgttgtgc ttttgagcat cctcatgctg
     5041 caaggatatc tgccgctaaa ccccagtaag tttcctggct tcacctggca tctcgccatc
     5101 aatacagctg taagctttgt caccaacacc aactggcagg catacagcgg cgaatcggct
     5161 gccagctatc ttactcaagc agcagggctg acagtacaga atttcgtctc agccgcaaca
     5221 ggaatggcta tcgtcattgc ctttatccgc gggctggcgc gcaggacagc agaaacgata
     5281 ggaaattttt gggtggacat gaccaggagc gttctctaca tccttttgcc gatatcactc
     5341 gtagcagctc ttgtactggt ttctcagggc gtaatccaga acttcagtcc ctacaagaca
     5401 gcccagcttg tggaggcaac aagttatgag aaacctaaaa ttgacgcgga aggcaacccg
     5461 ctcaaagacg aaaaaggaca ggccgttacc gagaaggtgg tagtgaagga acagaccctg
     5521 cctatggggc cggtggcctc tcaggaagca ataaaagaac tggggacgaa cgggggcggt
     5581 ttcttcaacg ccaattcggc gcatccctat gagaacccga cacccctgtc caattttctc
     5641 gaaatcctct tgattctctc gattcctgcc gccctaacaa atacattcgg ccgcatggtc
     5701 ggcaagacaa ggcagggatg ggccatttat acggcgatga tgttccttct ggtaatggct
     5761 gtaggcgctc tctattgggc tgaatacagc ggcaatcctt tgcttcaaaa gctgggtgta
     5821 cctggcatca acatggaagg caaagaggtt cgtttcggga ccggatggtc ggccttgttc
     5881 gccagcgcca ctaccgggac atcgtgcggg gcagtgaata ctatgcatga ctctttgacg
     5941 ccactcggcg gaatggtccc cctgatgctc atacttttga gcgaagttgt ttttggagga
     6001 gttggatcag gcctgtacac gatgcttgcc tttgtcatta tcgcggtctt cactgcaggc
     6061 ctcatgatcg gccggattcc tgagtatctt gggaagaaaa tcgaggtcac cgagatgtgg
     6121 atgtccgtga tcacggttct caccgccggt gtggtgatcc tccttttcgt tgccatagca
     6181 ctgattacgc cctcagccgt cagttccatg gccaacccag gtccccacgg cctgagcgaa
     6241 gtgctctatg cctttgcctc catgtccaac aacaatggca gcgccttcgc cggcctgaat
     6301 gcaaataccg tgttctataa cttgatgggg tctcttgcca tgctcgcggg aagatttgga
     6361 cccgctatcg cgatcctggc aatggcaggc tccctggtga agaagaagta tgttccagcc
     6421 agcgtgggca cgctgccgac agatcagctt cccttcatat tctggctcgt ctcggttgtc
     6481 ctgattgtcg gcgccctgac cttcttcccg gcccttgccc tggggccgat agtcgagcat
     6541 atgatcatgc agggaggtgt atagtcatgg cgaaacacat tgaaaaaagc aaaggaatat
     6601 ttgacccgaa attgatgaag gcggccctta tagattcctt caaaaagctc gacccccgag
     6661 ccctctggcg gaatccggtc atgctgtcgg tcgaaatcgg cagcgtgatc accacagtta
     6721 atttcatcta taatctggtc acagggaaag gggagccggc ctggtttacc ggaattgttt
     6781 cagcatggct ctggctgacc gtcatctttg ccacatttgc tgaatcgttg gcagaaggac
     6841 gaggtaaggc acgtgccgag tccatgagga agacccggaa ggagatcatg gcgaagaagc
     6901 tgaaaaagcc tgaattggga ggggaatatg aacttgtccc ctcgacccag ctccgcaaag
     6961 gcgacattat attggttgag gcaaaggata tgatcgccgg tgacggagaa gtgatcgcag
     7021 gcgctgcgct cgtcaacgaa tctgctgtca ccggtgaatc cgcgccggtt gtcagggaat
     7081 ccgggggcga ccgaagcgcg gtgactggcg gcactcaggt gatcgcaaat agcatagttg
     7141 tacgtattac tgcaaaccct ggcgagacct tccttgaccg aatgatctcc ctcgttgaag
     7201 gtgcgaagcg ccgaaagacc cccaacgaga tagccctgga ggtcctcctg gttgccatga
     7261 ccgtagtctt ctttcttgtc gtggtcaatc ttagtcccct ttccatttac agcgtaaaag
     7321 cggtggggca gggacagccg gtaaccctcg tggcgctggt cgcattattt gtctgtttga
     7381 tacccacaac cattgctgcc cttctgcccg ccattggcat tgcgggaatg gaccgcctct
     7441 ttcagaaaaa tgtcatagcc ctgtccggaa gggctattga ggctgccgga gatgtgaatg
     7501 tgctcttatt ggataagacc ggaaccatca cactcggcaa caggcaggcg gtcgctttta
     7561 ttccggtggg aggccattca gaaaaggaac tggctgaggc ggcattgatg gcttccctta
     7621 ctgatgaaac gcctgaagga cgaagcatcg tagtgcttgc caagcagaaa tacgagcttc
     7681 ggatgcggga attgccccac aatgccgagt cagtcccctt cagcgccgaa acaaggttaa
     7741 gcggggtcaa tgcagacggg catgcatatc gcaaaggagc gggagattct atcgtccgtg
     7801 ttgtccaggg catgggtgga actatcccgc atgatttgga aaaccatgtc actgatatcg
     7861 ccaaatccgg agggactcca ctggtggtga gtcgtgatac tgaggtcctg ggcgtcataa
     7921 acctgaaaga cattctcaaa ggtggaattc aggagcgctt tctccagctg cgcagaatgg
     7981 gaatcaagac agtcatgatc accggggata accctctcac cgcagcggcc atcgcgactg
     8041 aagcccaggt tgatgacttc cttgcccagg caaagcccga ggacaagctc aggctcatcc
     8101 gggagaacca gcagcaaggc tacatggtgg cgatgaccgg cgacggcacc aatgacgcgc
     8161 ctgcccttgc acaggcagat gtggcggttg cgatgaatac aggaacccag cctgcgcggg
     8221 aggctgccaa catcattgac ctggacagca atccgaccaa gttgctggat attgtggaga
     8281 tcggcaagca gatactgatg acacgtggta ccctcaccac cttcagcatc tcaaatgatg
     8341 tggcgaaata ttttgcgatc atccctgctg cgtttgccgc tgtctacccg cagctcggta
     8401 tcctcaatgt tatgcatctg gccaacccct tcagcgcgat cctttctgcc gtcatcttca
     8461 atgcgattat tattccgctg ctcatccccc tcgcattgaa ggggacccct taccggccga
     8521 tgcctgcgga aaggctgctg gtctataacc ttttgattta cggggtggga gggcttttgg
     8581 ccccctttgc agggatcaag cttatcgacg tattgataaa tgttctgatt tgaaaggagt
     8641 gacatcatga aagagttaaa gaacgggata ttactttttg tcgcattatc gttgttgacc
     8701 ggggtcctgt atcctgcagc ggtgaccgta ctggcacagc tcatgtttcc gcagcaggca
     8761 aatgggagta tcatgtatac agccgatggt atgccaatcg gatcgtcctt gatcggccag
     8821 cccttttctg atccgaaata tttctggccg aggccatcag caacgacgga tttcccttat
     8881 aatgccctgg catcgggtgg gtctaattta gggcccacaa ataaggacct gatcagtcag
     8941 gttgctgatc gggtaaaatc tttccgggaa tcgggtattc aagggcagct tccagccgac
     9001 ctggtgacgg cgtccgggag tggacttgac ccggatatca gccctgaagc ggcgctcata
     9061 cagatccctc gtatcgcaaa agagcggaat gtgagtgaag aaaaattgcg cgccatggtg
     9121 ctggcgcatc ttgaggaccg ccagtttggc ttcctcggga caccaagggt aaatgttctc
     9181 aaattgaatc ttgccctgaa caaggaatag gaaatggctg aagacgaaga atataaaaga
     9241 ccatctcctg aagcgcttct cgaaatggcc caaaaagagg aagctcagga aaaacggggc
     9301 aagctcacca tttacttcgg ggctgctcct ggggtaggaa agacctatgc aatgcttctc
     9361 gatgcacgcc agcgaaaaga ggagggcgtt gatgtggtgg tcggttatgt agagacccac
     9421 ggaaggtctg agaccgacgc attgctggaa ggcctcgaaa tcctcccgcc tctcgtcata
     9481 aactacatgg gagtccagct cagggaagtt gacctggcga aagtctttgt gagaaaaccg
     9541 aagctcgtcc tggtggacga gctcgcccat accgatgccc cgaatcttcg taatgccaag
     9601 agatatcagg acatagaaga aatactgaat gcgggtgtcg atgtctatac cactatgaac
     9661 gtgcagcacg tggagagcct taacgatatc gtccatcaga tcacgaatat ccgtgtgcgc
     9721 gagacagtgc ctgataccgt gattgaggca gcagacgaga tcaagctggt cgatctgcct
     9781 cctgaggaac tgcttaagag gctccacgag ggaaaggtct atgtaaaaga catggcaggc
     9841 agcgcggtgc aaaagttttt ccgtccgggc aacctgctgg cgctccggca gctggcgctc
     9901 cgggcagtgg caggcagcgt ggatgagaag atgcgtacat atatgcgtgc tcatgccata
     9961 gcgggaccat ggtctgcaac ggaccgtgtc cttgccgggg tatttgccag tccttatgca
    10021 gagaaactga tacgcgcagc attcaggctc gcaaactatc tggatgcgga atggatcgcg
    10081 ttctatgtgg agaccgaaag gcacaagaca ctttcagaga aggaaaagga gtggctgaat
    10141 aaggcgatgg acctggcaaa actgttggga gcacgcctgg tctggatcaa gggaactgat
    10201 gtgatggagg aaatggcgga ctatgcgcgg aacaacaatg tgaccaagat cgtcatcggg
    10261 aaaccccgtt ttgggctgtt tcagtctgtt cccaagaaac ttctgaccag aacacccaac
    10321 atcgacattt acctgttaga tgccagggtc ggtcccaggc acatgcccaa aaagcgtatg
    10381 atttttacca ggcccatgaa ttatgcgctc ggaatttttg ccgttggcat tgtgtcgctg
    10441 attggttttc tcttgcgcaa tgttctcaat gaggtcaatt tgctcttcct gcttttgttg
    10501 cccgtcattt tgagtgcgct ctatctggga aagggaccgt ccatcgtggc tgcgattacg
    10561 agcattctga tctttgacta cctctttgtc tctccccact tcagctttgc ggtgggcgat
    10621 gtgcaatatt tcttttcgtt cgtcgtgtac atcattgtgg tggtagtaat cagcaacctt
    10681 gcatccaagt tacgcggaaa agtggagatg ctcaagcaaa gcgagttgaa gaacataggg
    10741 ctttacggtc taagcaagga tctggtaacc gcccacacaa tagatcaggt gctctcactc
    10801 atggtgcgcc ataccctcga gatcttcccc tgcgaaatgg caatctttct gcctgaagag
    10861 ggaaacctta ctgtcaaggc aaaaacaccg gacttcgagg tgacccctaa actgctcggc
    10921 gtggcatcat gggtcatgat caacaaacag ccagcaggtc gcggcaccag tacattgcct
    10981 caggccactg catactacct gcccataatg tcaggcgagg aagccatagg ggtcgcaggg
    11041 ttcgatttca gggggagtga aggaatgatg acgagcgaga aaagggtggt tcttaaaacc
    11101 attacgcgtc tcggcgcaat ggcaatagaa cggatcagga tccaataata catggtcacc
    11161 gccaacaacg tagtatctga atctggcaac gacaacacgc cattctacaa atgggcgaac
    11221 tttcttgccc tgattactat cttttacaat cttgtggaag ggttggtttc tgtcttcttc
    11281 ggattggaag atgaaaccct tgcgcttttc ggattcggtg tggattcttt tgtggaggtg
    11341 atctctggtg tggggatatg gcatatgatc agaaggatga gacggaacgg aaatgaaaat
    11401 gctgacaggt ttgaacgcac tgcacttaaa ataacaggca cctctttcta tattcttacc
    11461 ttcggcctgc tcataatcgc tgcggtaagt atctccgagg ggtataagcc tgagaccaca
    11521 ttttggggca ttattgtgtc ggtcatctcc attcttacca tgtggctcct catgcattac
    11581 aaaataaaaa tcggcagcaa atttaattca caggctttgt tagcagacgc tgcctgcaca
    11641 aaaacgtgca tctgtctctc ggtaatcctc cttattgcaa gcgtgggcta tgaggtgacc
    11701 ggcataggca tgctggactc cttaggtgcc ttaggaatag ccatcctctc tttcaaagaa
    11761 gggagagagg catttgagaa gtcaagaggg aacttggcat gcagttgtca ggggcaatgt
    11821 cattagatgt gcgggagtgg caggattctc agccgccttt tacggctctg taaagagttg
    11881 ccacgcccat ggacattgat tggaacgtga cgtcagtgaa gccgcagcct ctcataagct
    11941 gtgtgaattc ctccggagga tagaacacat ctatggatct gggcagatag gtatagggca
    12001 cttcacttct cgtgataata gctgccactg caggcatgaa cttgaaggtg taccatttgt
    12061 aaaggggcag gaaccatcgt ttcgttggct tggtgagctc gaggcagagg aaccgccctc
    12121 ctggcttaag gacactgagg atcttcttca gggcaacttc ggtatcggga acgtttctca
    12181 tgccaaaggc aactgtgatg gcatcgaagg tattttcggg aaagctcatt tcccgcacat
    12241 ccgaaaccac aaaggtcatg ttcgcgggaa tggggtttaa cttccttttc gcgacctcaa
    12301 gcatctcctc gcagaaatca aggcaggtaa gagacccttc actgcctatc ctcctgataa
    12361 tcaatttggc cacctctcct gtacccgtac agagatccag gatatgttct cccctttcaa
    12421 tatcagaaac gagcctcctt ctccagaggt ggcagagccc catgctgaat gcggtatcga
    12481 ggggatcaat ataaggtgcg actattgaga agatatcccg tacagaggtc tctttttttt
    12541 cctgtggcat atcagactga gaccctttct attctgatcc tgcgcgagcc ctttgctttc
    12601 ctgaggagtt tggcatcacc ggtaattttt cccacgagat tcacttcgct ggcagggacc
    12661 ggctcagacc ctctgctcat cggcgtgggg ccaaaaaaga ttgcaagcgc ctttcccgga
    12721 ggccagtatc ccacatcccc gactttcacc ttcctggttg cggtctcgtc caggggcatc
    12781 tcaacgggta tcccgaaata aaactcatct ccccattcat caggcctcgt ttctatgggg
    12841 agagcctccg taattgcctt tgcacattga gtgtcgaaga gttctccgtc caacgcaata
    12901 tccgctatga cgatttttat cggtatcggc attatttctc ccttcctgtg gtaacttcgg
    12961 ttatcccttc ttgtatgacc cttaagagct gttcaagatt ctcggtgctg atggaaaggg
    13021 gcggcataat cactaccaca tttcccagtg gcctcagaaa gacccccttt gcacgcgcat
    13081 agtggcagac cctccacccc atcttttctt cccatgcata cggttctttt gtagatttgt
    13141 ctttcacaag ttccacacca gccattaatc ctttgttccg cacatcaccc acatgatcga
    13201 gttttgcaag gtcgtgcaag aaattttcga gaatccctat cttaaatccg aggccttcaa
    13261 tggtattttc cctctcaaag atgtcaaggc aggcaagtgc agcagcacat cccaggggat
    13321 tgcctgtata cgaatggccg tggaagaatg tcttaaggtc cctaaattca ccaaggaatg
    13381 cactgtagat tcccttattc gcaatagttg ctgccagggg gagatagccg ccagtgatgc
    13441 cctttgagag gcacatgata tcaggcacca catcctcatg ctcgcacgca aacaccctgc
    13501 ctgtcctccc gaagcctgtt gccacctcgt ccgcgatcat caggactctg tatttcgtgc
    13561 agagtttcct cacccctttg agatacccgg gaggcgctac aatcattccc cctgctgcct
    13621 gaaccagggg ctcaataatc accgccgcga tctctgatga gtgatccctc aggatctcct
    13681 ccatcttgcg taagcaggca aatccgcagt cagggtgcgt agcatccagt tcacatcggt
    13741 agcagtaggg tgaaggtgcc ttgtaagcct taaagaggag gggaccaaac gtgctgtgga
    13801 atatgtcaat gcctcccacg ctcactgcgc caagggtatc cccgtgatac ccgttcttca
    13861 aagacaaaaa agagcgcttg ccctccactc ccttgtgaat ccagtattgg aatgccattt
    13921 tgagggccac ctccactgca gtggagccat tgtcggaata aaagaccttg cttagagata
    13981 aaggattcga gggctcgatg gttcggggat tcgagggttt tatttcactt gaccccttga
    14041 ccccttgacc ccctgatccc ttttgattta ctaacttcac aagcctttcc gcaagttgta
    14101 tggcagggac attgctcagc ccgagcatgg tggtgtggct gatgcggtcg agctgtgcct
    14161 ttatggcgtc atcgatctct tttttcctgt gaccatggat attgacccag agcgacgaca
    14221 ctccgtccag ataccagttc ccatagatat ccttgagaaa gcagtccctc ccttcggtga
    14281 tgatgacagg tttctcgtcg agccactcct tcatctgggt aaagggatgc cagatgtagt
    14341 tcttgtctgt ttcttccaga tgtctgtttt cttcgatggt tcccatatgc aaacaatggc
    14401 aaagtcgtga aatggtttat catatcacaa aagctgtgcg ccatggcctt catttgtatt
    14461 ca
//
LOCUS       DATKOU010000039         8331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2357132, whole genome shotgun sequence.
ACCESSION   DATKOU010000039 DATKOU010000000
VERSION     DATKOU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357132"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1855)
                     /locus_tag="VMU21_01665"
     CDS             complement(68..1855)
                     /locus_tag="VMU21_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HUO76260.1"
                     /translation="MADEKPRDILSALTGPRPVDFTTRPVVMGTHGMVTSGHYLATRA
                     GLHILEEGGNAVDAGVATGFALSVLEPYLYGIGGEVPILLYLADMHRVVSLSGQGPAP
                     KAATIEWFKKRGMKNIPGDGLLAATVPDSVSTWITALANFGSMSLGKVLEPAIELAER
                     GFPMYGVLQSALHRNAERFLSDWPTSAEAYLPNGRVPSVGDLFAQPDLAETFKKLVSA
                     EHREIRRGRREALQAARDLFYRGKLAQQIIKFAQGSHPDASGARHNGLLAKKDLEEYS
                     TRMEEPVTTSYRGYDVYKCGPWTQGPVFLQQLNLLEGYDLLALGHNSAEYIHILVEAA
                     KLAYADRERYYGDPDFVKVPLNVLLSKEYAARRRRLIDPEQASLKLRPGNAAAGKPRA
                     KKGNPKVYTGDTTHLDVIDQWGNMLAATPSGGWFASSPVIKGLGFPLGTRLQVFFLDP
                     GHPNSLQPHKRPRTTLTPSLVLKSGRPSMVFGTPGGDQQDQWTLQFFLNCVDFNMNLQ
                     EAVDCPNFHSTHFPSSFDPHGAQPGGLVIESRISESTLSVLREKGHNIQVSGPWSNGR
                     VLGIRFDPWTGVISAAASPRMETGYAMGW"
     gene            complement(2115..3617)
                     /locus_tag="VMU21_01670"
     CDS             complement(2115..3617)
                     /locus_tag="VMU21_01670"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HUO76261.1"
                     /translation="MKAIILAGGSGTRLWPLSRKNYPKQFLKINACSDEGECAFDKGS
                     LIQQTVLRLLKAVTHDDIVIMTNGDYKFHVISDLNSMPGAGYEPLISNMILEPAKRNT
                     APAIALALRYCTDKLGCSGNEVVFISPSDHIIDPDPKFVQYLRAAERIAARGFIVTFG
                     IKPLYPETGYGYIRAKSPILRGKRYFEVESFTEKPDRKTAEKYLKQNGYFWNSGMFAF
                     RIDTVEEELKKHAPGIFELYTSGYQEMLASFDQMPDISIDYAVMEKSDRVVTMPMDIY
                     WNDVGSWDSIFSVFPKDKEGNVKIGDVLAHDTRDSLVISDRRLIATVGLDNILVVETD
                     DAILVARRGDAQKVKDIVEMLNAENRREASEHTTTYRPWGSYTVLEDGERYKIKRIVV
                     NKGETLSLQMHHHRSEHWVVIKGTARVTIGEEIKFIHENESIYIPKSTLHRLENPGKV
                     PLEIIEVQNGEYVEENDIVRLDDKYGRQRLAAKVKKNTRVRVRERKRGRR"
     gene            complement(3686..5107)
                     /locus_tag="VMU21_01675"
     CDS             complement(3686..5107)
                     /locus_tag="VMU21_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HUO76262.1"
                     /translation="MIRVEIRDEIFRQYDIRGMVGEDLDKEVAFLLGRAFSAYLKGIN
                     PEARKVSVGRDIRLSSDELAAGIRDGMLSAGLDVIDIGVCPTPLQYFSLFHLDLDGGI
                     MVTGSHNPPEYNGFKLSAGKGTIHGRAIQEIRQIIKNKDIDKGKRITGQLSVYEILPA
                     YREYMLKEFSYLSDPGFRRIKVVVDAGNGTAGLIAPDILSRMGCDVISLYCEPDGRFP
                     NHHPDPTVLEYIQDLITTTRESGADLGVGYDGDADRIGVIDSAGNVIWGDQLMIVLAR
                     DLLTRNPWAKIIGDVKCSQTMFDDIKMRGGVPIMWKTGHSLVKQKMRDEGALLAGEFS
                     GHIFIADRYFGYDDAIYTTFRLVEIIKKGGRGIKEILSDVPGMCFTPEIRMDCPEDLK
                     KRVVEGVVKRFLHYREEGGSPYPIKDVNTIDGVRVVFDKGWGLVRMSNTQPVMVMRFE
                     AEDEESLLNYRKFMEQEIRQVKI"
     gene            complement(5161..6105)
                     /locus_tag="VMU21_01680"
     CDS             complement(5161..6105)
                     /locus_tag="VMU21_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HUO76263.1"
                     /translation="MNKSSLIYIAGHRGLVGSAILKKLESEGYTNLVVKTSIELDLTR
                     QAEVEAFFQKEKPEYVFLAAAKVGGILVNNTYPADFIYDNIMIQTNIIHASYQTNVRK
                     LLFLGSSCIYPKQAPQPMREEYLLTGVLEPTNEPYAIAKIAGIKMCQSFNRQYATNFI
                     SVMPTNLYGPFDNFDLETSHVLPALIRKFHEAKVAAHPGPVVLWGTGTPKREFLYIED
                     LADACVFLMKRYDSSEIVNIGVGEDISIAELAALIGQIVGYEGGIIYDTSKPDGTPRK
                     LLDVSRLHSLGWKAKTGLREGIARTYEWYARHLKGQAG"
     gene            complement(6098..7183)
                     /gene="gmd"
                     /locus_tag="VMU21_01685"
     CDS             complement(6098..7183)
                     /gene="gmd"
                     /locus_tag="VMU21_01685"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HUO76264.1"
                     /translation="MKTALITGVTGQDGAYLAEFLLDKGYTVHGIKRRASLFNTDRID
                     HLYKDPHEKGVKFKLHYGDLTDATNLIRIVQEVQPDEIYNLAAQSHVMVSFETPEYTA
                     NADALGTLRLLEAMRILGMGKKTRFYQASTSELYGKVQETPQKETTPFYPRSPYGVAK
                     LYAYWITVNYREAYDFFACNGILFNHESPIRGETFVTRKITRAVARIKLGLQKKLFLG
                     NFNARRDWGHARDFVEAQWLILQQDKPEDYVIATGEQHSVREFVAAAFHEVGISIKWK
                     GKGSDEKGYDKDTGNVLVEVDRRYFRPAEVDTLLGDPTKSKKRLGWVPKVTFKELVSE
                     MVREDLKEAEKDQLCTREGFRTFRYHE"
     gene            complement(7269..7556)
                     /locus_tag="VMU21_01690"
     CDS             complement(7269..7556)
                     /locus_tag="VMU21_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76265.1"
                     /translation="MRKSVPGATFIIAGYGPPEDLTSLQKENPHVIVTCFVEDIDECC
                     KKASVVRLLKDPAQPAEIRKSGQYFVKTNVSLDSIISKIESAYGELVKRES"
     gene            complement(7603..8199)
                     /gene="cysC"
                     /locus_tag="VMU21_01695"
     CDS             complement(7603..8199)
                     /gene="cysC"
                     /locus_tag="VMU21_01695"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HUO76266.1"
                     /translation="MSERHVVWHECLVKRADRNSLNNHKSGLVWFTGLSASGKSTIAH
                     AVEKELHDRGIRTYVLDGDNVRHGLNSNLGFSPEDRKENIRRIGEVAKLMADAGIVVF
                     TAFISPYREDRDTVRKLFEGDHFYEVFVKCSIEECERRDPKGLYKKARAGIIKNYTGI
                     SSPYEEPLVPELIVDTEHLTIEESVNQVIGFLCERNLI"
BASE COUNT         1798 a         2253 c         2052 g         2228 t
ORIGIN      
        1 aattcagctc tatatgcagc gctactgata gttctgcgga tctgcgcctc agcgcagtgt
       61 gaggaattta ccatcccatg gcatagccgg tttccatgcg cggtgacgct gctgctgaaa
      121 tcactcctgt ccagggatca aaacgaatcc ccagcacgcg gccgttgctc cacgggcctg
      181 agacctgtat gttatgacct ttttcgcgaa ggactgaaag cgtcgattcg ctgatacggc
      241 tttctatcac gagaccaccg ggttgcgccc cgtgaggatc gaatgaactg gggaaatggg
      301 tggagtggaa gttcggacag tccaccgctt cctgtaaatt catattaaaa tcgacgcagt
      361 tcaaaaagaa ctggagcgtc cattggtcct gctgatcacc acccggcgta ccaaagacca
      421 tggacggtct gccgctcttg agcaccagcg aaggagtgag cgtggtgcgt ggacgcttat
      481 ggggctggag ggaattgggg tgcccagggt caaggaagaa tacctgaagt cgtgttccca
      541 acgggaagcc caaccccttg atgacaggag agcttgcaaa ccatcccccg cttggggtgg
      601 cagcgagcat gtttccccat tgatcaataa cgtcaaggtg tgtggtgtca cctgtgtaca
      661 ccttcggatt ccctttcttt gcccgcggct ttcctgcggc tgcattgccg ggacggagtt
      721 tcagggaagc ctgttcggga tcgatcagtc tgcggcgtct tgccgcgtac tctttggaga
      781 ggagaacatt caggggcacc ttcacgaagt ctggatcacc atagtagcgc tcacggtccg
      841 cataggcaag ctttgctgcc tcgacgagaa tatgaatgta ctcggcagaa ttatgaccaa
      901 gggcaagaag gtcatatcct tccagcagat tcagctgctg caggaagacg gggccctggg
      961 tccatggtcc acacttgtag acatcataac cgcggtaact ggtagtcaca ggctcttcca
     1021 tgcgggtcga gtattcctca aggtctttct tcgccaggag accgttatgg cgggcgcccg
     1081 aagcatcagg gtgagaaccc tgggcaaatt ttatgatctg ctgtgccagt tttccgcgat
     1141 aaaacagatc tctcgctgcc tgaagcgcct ctctccttcc gcgacgtatc tcacgatgtt
     1201 ctgcgctcac gagtttcttg aacgtttctg ccagatcagg ttgcgcgaag aggtccccca
     1261 ctgatgggac ccttccgttg ggaagatacg cctcagcgga ggttggccag tcgctcagaa
     1321 acctttcggc gttcctgtgg agagcagact ggagtactcc gtacatggga aatccccgtt
     1381 ctgcaagttc aatggcaggc tccaatacct ttccaaggga catgctaccg aagttcgcca
     1441 gggccgtgat ccatgttgac accgaatccg gcaccgtggc agcgagcagg ccgtcgccgg
     1501 gaatgttttt catccctctt ttcttgaacc attctatggt cgctgccttt ggggcgggtc
     1561 cctgtccgct caggcttact acacggtgca tatcggcaag gtaaaggagg atcggcactt
     1621 cacctccgat accgtaaaga taaggttcaa gcacggagag ggcaaatccc gttgcaacgc
     1681 ctgcgtccac tgcattgcct ccctcctcca gtatatgaag accggctcgg gtggcgaggt
     1741 aatgaccact ggtgaccata ccgtgggtgc ccatgaccac aggccgggtc gtaaaatcaa
     1801 caggccgggg acctgtgagt gcgctgagaa tatctcttgg cttctcatct gccatatatc
     1861 acacccctgc gtacattaat cagctgtcaa agagattaat gcttcctttt caatattgtt
     1921 cttgagagct ttttgaacca aaaacctgat atctgaacaa catcttttgc cgctccggtg
     1981 agacagacct tgtttttcat tatgcggtga atccttatgg cttgtgacaa aaaaaattat
     2041 aataataatc ctgatagtcc gtcaagctct cccccgctta caagtccgga actcctcagt
     2101 ccggcaggca tttcttatct ccttcctctc tttctttccc tgacccttac cctggtattt
     2161 ttcttgactt tggctgccaa ccgctgccgc ccgtatttat cgtccagcct cacgatatca
     2221 ttctcttcga cgtactcgcc attctggacc tcgatgatct cgagagggac cttcccggga
     2281 ttttcgagcc tgtggagggt cgattttggt atgtagatgc tctcgttttc atgaatgaat
     2341 tttatctctt caccaatggt cacccgggcg gtccccttga tcacgaccca atgttcgctc
     2401 ctgtggtggt gcatctggag gctcaacgtt tcccctttgt tgaccacaat cctcttgatc
     2461 ttgtaacgct cgccgtcttc caggaccgtg taacttcccc aaggtctgta tgtggtggtg
     2521 tgttccgatg cctcacgccg gttctctgca ttcagcatct cgacgatgtc tttgaccttc
     2581 tgggcatcac cgcggcgtgc cacgagaatg gcatcatcag tctcgaccac gaggatgttg
     2641 tccaacccaa ccgtggctat cagtctcctg tcgcttatga caagggagtc ccttgtatca
     2701 tgggcaagga catcccctat cttcacattg ccctccttgt ccttcgggaa gacagagaat
     2761 atggagtccc aggagccgac atcgttccag tagatgtcca tgggcatggt gacgactctg
     2821 tcggactttt ccatcaccgc ataatcaatg gagatgtcag gcatttgatc gaaggaggcc
     2881 agcatttcct gatatcccga tgtatagagt tcaaagatcc cgggtgcgtg ctttttcagt
     2941 tcctcttcga cagtgtcgat cctgaaggcg aacatgccgg aattccagaa gtatccgttc
     3001 tgtttcaggt atttttctgc tgtcttgcgg tcaggttttt cggtaaagct ttcaacctcg
     3061 aaataccttt ttcctctgag tatagggctc tttgctctta tatacccata ccctgtttcc
     3121 ggataaaggg gtttgatacc gaaggtgacg atgaagcccc tggctgctat ccgttctgca
     3181 gccctcaggt actggacgaa cttcgggtcc ggatcgatga tatggtcaga gggtgagatg
     3241 aagacaactt cattaccgct gcagccgagc ttgtcagtgc agtatctcag ggccagggca
     3301 atggcaggcg cggtgtttct cttggcaggc tcaagaatca tattggagat gagcggctca
     3361 taaccagccc cgggcatgga gttcaggtca gagatcacat ggaatttata gtcaccgttt
     3421 gtcatgatca cgatgtcatc gtgggtgacg gccttcagga gccgtaagac cgtctgctgg
     3481 ataagggacc ccttgtcaaa ggcgcattcc ccttcatcgc tgcacgcatt gatcttgaga
     3541 aactgcttgg gatagttctt cctcgaaagc ggccagagcc gggttccgct tccccctgcc
     3601 agaataattg ccttcattga atccctccta aagagccaag cttgagatat aaacaggttg
     3661 aggaaatacg tttactcacc ttaacctata tcttcacctg tcttatttcc tgctccatga
     3721 acttccgata gttcaggagg ctttcctcgt cttctgcctc gaatctcatg accatgaccg
     3781 gctgggtgtt gctcatcctc accaatcccc atcccttgtc aaaaacgacc ctcaccccgt
     3841 ctatggtatt cacatcttta atgggatagg gactgccgcc ctcttccctg taatggagaa
     3901 accgctttac gaccccttcc acaacccttt tcttcagatc ctcggggcaa tccatgcgta
     3961 tttccggggt aaaacacatg ccggggacat cggagaggat ctccttgatg ccccttccgc
     4021 ccttctttat gatctccacc agcctgaagg ttgtatagat agcgtcgtcg tatccgaagt
     4081 acctgtctgc gatgaaaata tggccgctga actccccggc gaggagcgcc ccttcgtccc
     4141 tcattttctg cttcaccagg gaatgtcctg ttttccacat gatgggcacg ccgccacgca
     4201 ttttgatatc atcgaacatt gtttgcgagc atttcacatc gccgatgatc tttgcccagg
     4261 gattccgggt gagcaggtct ctggcgagaa ctatcatcag ctggtctccc catatgacat
     4321 tccccgcact atcaataacg ccaatcctgt ccgcatctcc gtcatagccc actccgaggt
     4381 ctgcccctga ttccctcgtg gtcgtgatga ggtcctgtat gtactcaagc accgtgggat
     4441 cgggatgatg gttgggaaat ctgccgtccg gttcgcagta aagggatatg acgtcacatc
     4501 ccatcctgct caggatatcg ggcgctataa ggccggcagt gccgttaccc gcatccacca
     4561 cgacctttat tcttctgaac ccggggtcgc tgagatagga gaactctttc agcatgtatt
     4621 cccggtatgc aggaagtatc tcatacacgc tcaactgacc tgttattcgt ttgcctttgt
     4681 ctatgtcttt gttttttatt atttgtctga tttcctggat agccctgccg tgaatggtcc
     4741 ctttaccggc gctcagcttg aagccgttgt attcaggcgg gttgtggctt ccggtgacca
     4801 tgatgcctcc gtccaggtca aggtgaaaaa gggaaaaata ctgaagcggg gtgggacaga
     4861 caccgatgtc gatcacatca agccccgcgg ataacatgcc atccctgatt cctgccgcga
     4921 gttcgtcaga actgagtctt atatcgcggc caacgctcac cttgcgtgcc tcggggttga
     4981 tgcccttcag gtaggcagaa aaagcccggc ccaaaagaaa tgcgacctcc ttgtcgaggt
     5041 cttctcccac catccccctg atgtcatatt gtctgaatat ctcgtccctg atctccaccc
     5101 taatcacaca cctgaccgta tacttaacct gagggtttgt atgctcgcag gattttgcac
     5161 ttatccagct tgccccttaa ggtgtcttgc ataccactca tatgttctgg ctatgccttc
     5221 tcttagacct gtttttgcct tccagcccag ggagtgcaac cttgatacat ccagcagctt
     5281 tctgggcgtt ccatcaggct tggaggtgtc atagatgatg ccgccttcat accctacgat
     5341 ttgcccgatg agggctgcga gttcggcgat ggaaatgtcc tcgccgactc ctatgttcac
     5401 aatctctgag ctgtcgtatc tcttcatcag gaaaacgcag gcgtccgcaa ggtcttcgat
     5461 gtagaggaat tccctcttgg gtgtccctgt cccccagaga acgacaggcc cagggtgcgc
     5521 agcaaccttc gcttcatgga attttctgat gagggcaggg agtacatggg aggtctcaag
     5581 atcaaagttg tcaaaaggac cgtaaagatt cgtgggcatc accgagatga agtttgtagc
     5641 atactgcctg ttgaaagact ggcacatctt tattcctgcg atctttgcaa tggcataggg
     5701 ttcattggtc ggctccagca ctcctgtgag aaggtactct tccctcatgg gctggggggc
     5761 ttgcttggga tatatgcagg aacttccgag aaagaggagc tttctgacat tggtctgata
     5821 ggaagcatga atgatgttgg tctgtatcat gatattgtca tagataaagt cggcaggata
     5881 ggtgttattg acaagtatac cgcccacctt tgccgctgca aggaagacat attccggttt
     5941 ttctttttgg aagaaggcct ccacttcagc ctgtcttgtg aggtcaagct ctatgctcgt
     6001 cttcactaca aggtttgtgt acccctcaga ttcgagcttt ttgagaatag ctgaacccac
     6061 gagtccgcga tgtccggcga tgtaaatgag ggaagattta ttcatgatat ctgaaggtcc
     6121 tgaacccctc tcttgtgcag agctggtcct tttccgcctc ttttaggtcc tccctcacca
     6181 tctcagagac cagctctttg aaggtgactt taggcaccca tcctagtctt ttctttgact
     6241 ttgtagggtc tccaaggagg gtgtcaacct cagcaggccg gaaatagcgg cggtccactt
     6301 ccacgaggac gttgccggta tctttgtcat atcccttctc atcgctccct ttgcctttcc
     6361 atttaatgct gataccaacc tcatgaaatg ccgccgccac aaactccctc acagagtgtt
     6421 gctcaccggt ggcaatgaca tagtcttctg gcttgtcctg ctggagtatc aaccactggg
     6481 cctcaacgaa atcccttgca tgtccccagt cacgccgcgc gttaaaattg cccagaaaga
     6541 gcttcttctg cagccccagc tttatccgcg ccactgctcg ggtaatcttc cgcgtaacaa
     6601 aagtctcgcc gcgtatgggg gattcatggt tgaaaagaat tccgttacac gcgaagaagt
     6661 cataggcctc gcggtagttc accgtgatcc aatatgcata gagttttgca acgccgtagg
     6721 gactcctcgg ataaaaaggt gtcgtctctt tctgcggggt ctcctgtact ttaccgtaaa
     6781 gctcggacgt tgaggcctgg taaaagcggg tcttctttcc catccccaaa atcctcattg
     6841 cctcgagcag cctcagggtc cccagggcgt cggcgttggc ggtgtattct ggtgtctcga
     6901 aggagaccat gacatggctc tgggcagcga ggttatagat ctcatccggc tgcacctcct
     6961 ggacgatgcg gatgagattc gtggcatcag taagatcacc gtaatggagc ttgaacttca
     7021 cccccttctc atggggatct ttgtagagat ggtcgatcct gtcggtattg aagagtgagg
     7081 ccctcctttt tataccgtgg accgtatatc ctttgtcgag aagaaactcg gcaagatatg
     7141 cgccgtcctg tcctgttacg cctgtgatca atgcggtttt catttgtttc ctcatttctt
     7201 aagttataag acttagcttt atacggtttt tcaattctat cataaacctt tgtgttacgg
     7261 ctcacacttt acgattcacg cttgacgagt tccccgtatg ccgattcaat cttgctgata
     7321 atgctatcga gggaaacatt tgtcttcaca aagtactgcc cgcttttcct gatttccgct
     7381 ggctgtgcag ggtctttcag cagcctcacc acagatgcct ttttacagca ttcatcaatg
     7441 tcctccacaa aacaggtgac aataacatga gggttctctt tctggagaga ggtgagatct
     7501 tcaggggggc cgtagcccgc gatgataaac gtcgcaccag ggacgctctt gcgcattagc
     7561 gggaagacct ccagatagaa gaagagggct tacccttgtc gatcaaataa gatttctctc
     7621 gcagagaaag cctatgacct gattgaccga ttcctctatg gtcagatgct ctgtgtcgac
     7681 aatcagctcg gggacaaggg gctcctcata aggcgaagaa atgccggtgt agttctttat
     7741 gatgccggca cgggctttct tgtaaagccc cttggggtcc ctcctctcgc actcttcaat
     7801 ggaacacttc acaaagacct catagaaatg gtcaccctcg aaaagctttc tcacagtatc
     7861 cctgtcttct ctgtagggag atatgaaggc agtaaagacg actatgcctg cgtccgccat
     7921 cagctttgct acttcgccga tcctgcggat gttctccttc ctgtcctcag ggctgaagcc
     7981 gagattggag ttcagtccgt gccttacatt gtcgccgtcc aagacatacg tcctgatacc
     8041 ccggtcatga agctcctttt cgaccgcatg ggcaatggtg gatttcccgg aggcagagag
     8101 gccggtgaac catacaagac cgctcttgtg gttgttgagg ctgtttctgt ctgcccgttt
     8161 tacaaggcac tcatgccata ctacatgtcg ttcgctcatt gatctctcct tcatcagaaa
     8221 tactaaatag taaacagtga aatgtcaaag acaagctaat acgttgttct tccatgtgga
     8281 cagccctagg tgcctctcac cagattacag gcaacgaatt caggagtggt t
//
LOCUS       DATKOU010000040         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1974861, whole genome shotgun sequence.
ACCESSION   DATKOU010000040 DATKOU010000000
VERSION     DATKOU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974861"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..717
                     /locus_tag="VMU21_01700"
     CDS             151..717
                     /locus_tag="VMU21_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO76267.1"
                     /translation="MDKLVEILTKKYPRKVVLDDKSEVTFRPLRKDDEMALSDFFRDL
                     PLNDRACLKEDVADPKVIENWIYNLDYDNVLPVIATHDGTIAGDATLHFNPIGWTKHQ
                     GEIRLTTDTHYRGKGLGTMLAQEIIDIAKQLGLELLSIEMAPGLFEAYSLSEKLGFTK
                     AAVLKGFIIDLDGKETDLILMTMHLNGS"
     gene            857..1747
                     /locus_tag="VMU21_01705"
     CDS             857..1747
                     /locus_tag="VMU21_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUO76268.1"
                     /translation="MDKEQMYDCIIIGAGPGGLQAAIYLGRYNRKVLLINRGGGRTSH
                     AKSIENFLTQKEISGGEMIRLGLEQARSFNVRVEQGTVTNIIKREYFEVHAGETIYHT
                     LFVIVSTGATENFPPIDRIRKFLGTGFYTCVDCDGYKTMGKKLVVLGTSLKTLHLAFG
                     MKEMYTKDVTVVLYNQTVPEEYRKELRDEDIRLITGRPVRIVGQDRIEGIEMRDGQVV
                     ECEAIMSNFGFKLNDEFLSNLNLKRDRDNFKYITNSNCESSMDRLYMVGPLTGNDQAI
                     IAAGEGAIAAIDLKRRLLER"
     gene            complement(1856..2095)
                     /locus_tag="VMU21_01710"
     CDS             complement(1856..2095)
                     /locus_tag="VMU21_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HUO76269.1"
                     /translation="MKKVALSEVKDDLSKFLRLAEKEEVVITRHGKPAGVLIGFEDEE
                     GWFEYRLENDPRFLKRIEQARKSLKSGLGIKIEDV"
     gene            complement(2092..2385)
                     /locus_tag="VMU21_01715"
     CDS             complement(2092..2385)
                     /locus_tag="VMU21_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO76270.1"
                     /translation="MKYKIILAPEAVQDLKLLDAYQRAKIKDFIEVHLRHEPAKSSKG
                     RIKRLRGLRRPQYRLRIDDFRIFYDIDEDRVEILAIILKSKASEWLKKAGEKI"
     gene            2429..2560
                     /locus_tag="VMU21_01720"
     CDS             2429..2560
                     /locus_tag="VMU21_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76271.1"
                     /translation="MAASRRRLSLVDCVSFEVMRTLGIRTSFSFDPLFKEQGFTLLP"
BASE COUNT          838 a          787 c          774 g          769 t
ORIGIN      
        1 cagaaacgtg atgtgaaggt gggattggca gccctctgcg tgggcggggg aatggggttc
       61 gcgatgatcg tcgagcgggg gtggtacttc tgatgcctgc acccttgatc gcccttcaat
      121 gtgggtacaa tggattctaa ggagacggca atggacaagc ttgtggaaat actgaccaaa
      181 aaatacccca ggaaggtcgt cctcgatgat aagagcgaag tgaccttccg gcctcttcgc
      241 aaggatgacg agatggccct ctcggatttt ttcagggacc tccccctcaa tgacagggcg
      301 tgcctcaagg aggatgtggc agacccgaag gtgattgaga actggattta caatctggat
      361 tacgacaacg tccttcccgt catcgcgacc cacgacggga ccatcgcagg ggacgccacc
      421 ctgcacttca accccattgg atggacaaag caccagggcg agatccgatt gaccactgac
      481 acccactacc gggggaaggg cctcgggacc atgctcgccc aggagatcat cgatatcgca
      541 aaacagctgg gactggagct gctcagcatc gagatggctc ccgggctgtt cgaggcatac
      601 tcactctctg agaagcttgg gttcacaaag gcagcggtcc ttaaagggtt tatcatcgat
      661 cttgacggga aagagacgga ccttatcctc atgaccatgc atctgaatgg atcataactg
      721 gggaatgccg aagtccctca cttaactcgc atacgacatc ctgcttctgc ggtgcggagt
      781 ccattccaaa agcgaaaaga cggttccgaa aagtcctcac acaaatccgc cgaagagttt
      841 tctgttaaaa tgccttatgg ataaagaaca gatgtatgac tgtatcatca taggcgcagg
      901 accagggggc ctccaggcgg ccatctatct cggccggtat aacaggaagg tgctcctgat
      961 aaaccggggc ggcggaagaa cttctcatgc aaaatcgata gagaatttcc tgacccaaaa
     1021 ggagatatcg ggcggggaga tgatacggct ggggctcgaa caggcgcgga gcttcaatgt
     1081 aagggtcgaa caaggcacgg tcaccaatat tattaaacgt gaatacttcg aggtgcatgc
     1141 cggagaaaca atctatcata ccctattcgt aatcgtatcc acgggcgcca ccgagaactt
     1201 cccgccgata gaccgcatcc gcaaattcct tggaaccggc ttttacacct gtgttgattg
     1261 cgacgggtat aagaccatgg gcaagaaact ggtggtactg gggacctccc tcaaaaccct
     1321 ccatctcgcc ttcggcatga aggagatgta cacgaaagac gtcaccgtcg ttctctacaa
     1381 ccagacagtc ccggaggaat acaggaaaga gctcagggac gaagacatcc gcctgatcac
     1441 gggtcgcccg gtgcggatcg tcggacagga caggatcgag ggaatagaga tgagagacgg
     1501 tcaggtcgtg gaatgcgaag ccatcatgtc gaattttggc ttcaagctca atgatgaatt
     1561 cctctcgaat ctgaatctca aaagggacag ggacaatttc aaatacataa ccaattcgaa
     1621 ctgcgaatcc tcgatggacc gcctctatat ggtcggccct ctcacaggca acgaccaggc
     1681 gatcattgcc gcgggagagg gcgctattgc tgctatcgat ctgaagagga gactgctgga
     1741 gcggtaatgg accagcggtg actggtgatg cctcaatatt gattacaaac gaaaatctct
     1801 cacgtctgaa ctgtgcggtg gcgcaacgcc aaaggcacga gtgatttgtt atgccctaca
     1861 catcttctat ttttattccc aagccagatt tgaggctttt acgagcttgc tcaattctct
     1921 ttaagaatcg cgggtcattc tccagacgat attcaaacca accctcttcg tcttcaaacc
     1981 cgattaaaac accggcgggt tttccatgcc gcgttattac aacttcttct ttttctgcta
     2041 accgcagaaa tttggaaagg tcatctttca cttctgacaa agccaccttt ttcatatttt
     2101 ttctcctgcc ttcttaagcc attcagaggc ttttgatttt aagattattg caagtatttc
     2161 aactctgtct tcgtctatat cataaaatat tcgaaagtca tcgatcctaa gtctgtattg
     2221 aggacgccgt aaacctctca atctctttat tctgccctta ctcgacttgg ccggctcgtg
     2281 ccttaaatga acctcaataa aatcctttat ctttgccctt tggtaagcat ccagcagctt
     2341 caagtcttgg acagcttccg gcgccaatat gattttatat ttcattctgg ccatattata
     2401 gccaaattag aaagatatca tcaagttgat ggcggcgtcg aggcgcagac tgagccttgt
     2461 tgactgcgtg agcttcgagg tcatgcgcac cctcggcatc aggacatcct tctcattcga
     2521 tccccttttc aaggaacagg ggtttacgct tcttccttaa gccgtcataa ccggatcggg
     2581 cagacatacg aacaggcgct gagttcaggt ttctgttaac atggaacgtg tcaagaagag
     2641 cagcgacaca aaacaggcta ggatacacgg agccatttcc gcttgctgtc cgaggctggt
     2701 ataagaactt gctgtttcag attcttaaca atattggctg cacctgaagc tcctcactga
     2761 aacgattaga ttaggcatga gaagttctgt ttgaaacagt caaggactcc ccctcattcc
     2821 ctctgtctca atcttttccc ttccctcgtg tagtaaaata attctatggg gtcacttttg
     2881 ataaataatg tatttgacgc attgcgaaag cacctatatg atcatattga tcggctgcag
     2941 tgcttcggca aatatgagtt tcaggctgaa ggttggctta aagccgagtg tgtcttcgtc
     3001 cttgacgacc tgaaaaaccg aaaactcatc tgcaatgttg atcgcgccag ttcaacaatg
     3061 aagtgcaaca cttgggagag caggaaagga gatgctatcc taagtgtcta tgccaaagaa
     3121 ctacaaacat cttaccatgg acgaaagaga agtcatagcc aaaatgca
//
LOCUS       DATKOU010000041         7738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2358415, whole genome shotgun sequence.
ACCESSION   DATKOU010000041 DATKOU010000000
VERSION     DATKOU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358415"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..368)
                     /gene="hisI"
                     /locus_tag="VMU21_01725"
     CDS             complement(51..368)
                     /gene="hisI"
                     /locus_tag="VMU21_01725"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HUO76272.1"
                     /translation="MIPKLKYDEKGLIPAIVQDVDNGDVLMMAYMNESSLKMTLETGF
                     THFWSRSRQKYWKKGETSGCLQEVREVCYDCDEDTLLIKVRQHGAGACHTGTRTCFFR
                     KIE"
     gene            complement(823..1191)
                     /locus_tag="VMU21_01730"
     CDS             complement(823..1191)
                     /locus_tag="VMU21_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76273.1"
                     /translation="MEAAKIYGLCLERIAHQIRIAKQRINGGHPHNGQVLLESYNSGD
                     IEDIIDILEFYDIEVRTNEALKEKLEDLAYTVSELMREVLFFDYTEEGHIGFYLSLKD
                     DSGDELHERIDAGAVAVAVL"
     gene            1375..2727
                     /locus_tag="VMU21_01735"
     CDS             1375..2727
                     /locus_tag="VMU21_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO76274.1"
                     /translation="MPQTVTEKIILFTKDDSLREELTDLLPKGFTLSNQDSADKGCVF
                     FDIDTMKAGLMKGLANEHIVIAITKQKRTEPVMEATTFGAYEVMHRPLKDDVVLQLLK
                     ELKDFGNETRDTIPVTTLPPTPTCAIVGHSPIVMDVCKRLARLSQVDSPVLVTGETGT
                     GKELIAESIAQLSSRFGKPFVVVNCAAVPENLLESELFGFEKGSFTGAVSAKEGMLKI
                     ADQGCVFFDEIGELPLSLQGKLLRFLQTQTFYPIGSTKEIQVNVRVISATNRDLSGMV
                     REGKFREDLYHRLRVTSIHIPPLRDRKKDIPPLVHFFLNKYKHTAPRPIKGITKEFLG
                     KLVSYDWPGNIRELENTIRSAMALSKTHYLTTHELRELGSHPASKKTPSSETLASALV
                     PLIKEAIEKKDKNLYERIHSEVDKTVLEYVLSRTKENQSEAARILGINRLTLRKKLRF
                     "
     gene            2837..3439
                     /locus_tag="VMU21_01740"
     CDS             2837..3439
                     /locus_tag="VMU21_01740"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HUO76275.1"
                     /translation="MMEKQVLVIATRNRKKVEEIRRIMEGVAVELLSLDDFPGCPEVA
                     EDADTFEGNAEKKARAVASFTGKPSVADDSGLEVYALKGAPGVLSARYAGTGADDKKN
                     LGKLLAELQGTEEGRRGGRFVCVIALAFPNGRVETFHGVAEGRIGTEPKGFNGFGYDP
                     VFYPKGHHTTFAEMSADEKDALSHRGIALRKLRSYLSSVT"
     gene            3659..4306
                     /locus_tag="VMU21_01745"
     CDS             3659..4306
                     /locus_tag="VMU21_01745"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HUO76276.1"
                     /translation="MRLVLLGAPGAGKGTQAKKLIEKYAIPQISTGDLLRAAVAAGTP
                     LGKEAKTFMDKGELVPDRVVLGMVEERLKRDDCKKGYILDGFPRNTAQAEALDKMLTP
                     LGMSLSAALSVDVPFDDLMKRLTGRRTCKACGQMYNIYSNPPKKEGICDKCGGALFQR
                     DDDKEETIKKRLEVYNAQTAPLMDYYRKKGILSSVKGTGSIDDIFHAVCKSLGLK"
     gene            4620..5828
                     /gene="sat"
                     /locus_tag="VMU21_01750"
     CDS             4620..5828
                     /gene="sat"
                     /locus_tag="VMU21_01750"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HUO76277.1"
                     /translation="MALVNPHGKEKKLKPLLLVGKELEEEKKKAKTLKQVKISSRESG
                     DLIMMGIGGFTPLDGFMTYEDWKDVCSDYKMADGTFWPIPVTVSTDENIKAGTEVALY
                     SDEYGEVMATMKVSEAYKIDKEFECKNVYRTTDMKEHPGVKMVMDQPGTNLAGPVKVL
                     SQGTFPTEYPNTYMTPAQTRHLFLQNGWKKVAALQLRNPMHRSHEYLAKIAIETCDGV
                     LIHQLLGKLKAGDIPAEVRVQAIDKLVEHYFVKNTCIQAGYPLDMRYAGPREALLHAL
                     FRQNYGCSHMIIGRDHAGVGEYYGPFDAQKIFDEIPEGALETQPLKIDWTFYCTKCDG
                     MASMRTCCHGKEDRMLLSGTKLRKMLSENEEVPDHFSRPEVLAILRKYYAGLTEKVEI
                     KTHKYSEGIK"
     gene            5993..6412
                     /gene="aprB"
                     /locus_tag="VMU21_01755"
     CDS             5993..6412
                     /gene="aprB"
                     /locus_tag="VMU21_01755"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HUO76278.1"
                     /translation="MPSFVITEKCDGCKAQDKTACQYICPHDLMTLDREKMKAYNQEP
                     EQCWECFNCVKICPTQAIEVRGYSDFVPLGSSTIPLRGTDSIMWTIKFRNGILKRFKF
                     PIRTTAEGSVDPYAGKPEPDFATLKQPGLFTAAARKE"
     gene            6426..>7738
                     /locus_tag="VMU21_01760"
     CDS             6426..>7738
                     /locus_tag="VMU21_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUO76279.1"
                     /translation="MEKETCTFSFCKKPEVTEVACDLLIMGGGMAGCGAAFEACRWAN
                     AKGLKVVMVDKAATDRSGAVAMGLSAINTYVGENKVADYVKYVRNDLMGIIREDLVFD
                     LGRLVDDSVHLFEEWGLPIWKKGDDGFSIDGHEAKEKGKPMLKDGGTPVRSGKWQIMI
                     NGESYKVVVAEAAKKALQTNREKTGMAQNHFERVLITTPLMDVNGKNVAGAAGFSVRE
                     NKIYAFKAKAIINATAGAVNCFRPRSVGEGMGRTWYPVMSAGSGYAFGMLAGAELTLM
                     ENRFVPARFKDGYGPVGAWFLFFKAKATDSLGNDYCADKEYFDDAVARYGDYAKGLGT
                     AIRNHLMMRAMRDGRGPILMQTPAAMEALGKAFKEKLGDKEGAKKMKHLEAEAWEDFL
                     DMAIGQASLWAASNVEPDKVVSEIMPTEPYLLGSHAGCAGFWVSGP"
BASE COUNT         2093 a         1751 c         2058 g         1836 t
ORIGIN      
        1 gagctatctt gcctcggacc gggcgaccca ctacggctat gagccatcag ctattcaatt
       61 ttcctaaaaa agcatgtcct ggtccctgta tgacacgcgc ctgcgccgtg ctgtctcacc
      121 ttgatcagga gggtatcttc gtcgcagtcg tagcatacct ccctcacctc ctgcagacag
      181 cccgaggtct ctcccttctt ccagtatttc tggcgtgagc gggaccagaa gtgggtgaat
      241 cctgtctcca gggtcatttt cagggaggat tcattcatgt atgccatcat caggacgtca
      301 ccattatcca catcctggac aatggcagga atcagtccct tttcatcata tttcaatttg
      361 ggaatcatgc gtttccttaa tatattttgt atgcgaagta ttgtatcaaa tttcagattt
      421 ttaagacagg gagaatatcc cggtgattct ggacagaaat tctcaagact gcattcagaa
      481 gaacgtgggg ttcatggttc gttgactgga ggcttcctct attccgcagg ttttagcaag
      541 agaagcgatc aaggataaag gtacttttgt tcattctctg tggggtctca accaaaccaa
      601 agaggcggtg cctcagcttc ttgtcgctag ttcttggtat ggtgaagaca gcctgtcaga
      661 caacgctggc atgattcgag tgtgaaagaa catatttcct gatgtccgag gacctttcgt
      721 tcgcaacact tttcacataa ctcgagggtg aagggacatg tttctcgttt cactttgctt
      781 cccatcacca tctttccctc cctgactaag agtgtttgtg gattataaca cagcgactgc
      841 aactgcacca gcatcaatcc tctcatgtaa ttcatcaccg gaatcatcct ttagagaaag
      901 ataaaaccct atatggcctt cctctgtata atcgaaaaac aagacttcac gcataagctc
      961 tgaaacggta taggcaagat cttccagttt ctctttcagc gcttcgttcg ttctcacttc
     1021 tatatcgtag aactcaagaa tatcgatgat atcctcaata tcaccggagt tgtaggattc
     1081 gagcaagacc tggccattat gcgggtgtcc tccgtttatc ctctgttttg caattctaat
     1141 ttggtgggct atcctttcca gacagagacc gtatatcttt gctgcttcca ttttgttccg
     1201 ctcctttcgc acgttttgaa ttccttatga atttgcaagc tgcatgccat atgatacatg
     1261 ccgtaaataa agagattttt tggggggagg atttcttgtt gattattatc tatacatggt
     1321 gataacataa taaccaggca atttggctaa gattgcgcac tatgagcact gtgtatgccg
     1381 cagaccgtga cagagaagat catactattt acaaaggatg actccctgag ggaggagtta
     1441 acggacctcc tgccgaaggg cttcacactt tccaatcagg attctgctga taagggctgt
     1501 gtcttctttg acatcgacac gatgaaagca gggctcatga agggtttggc gaatgaacat
     1561 atcgtcattg cgatcacgaa acagaagaga acagaacctg ttatggaggc aacgaccttc
     1621 ggagcctatg aagtcatgca tcgcccgctg aaagatgatg tcgtccttca gcttcttaag
     1681 gagcttaagg actttggcaa tgaaacaagg gataccatcc ctgtcacaac ccttcctccc
     1741 acgcccacgt gtgccattgt cgggcactcc cctatcgtca tggatgtatg caagagactt
     1801 gcgcgcctgt ctcaggtgga ttcacctgtc cttgtcaccg gtgaaacagg gacagggaag
     1861 gagcttattg cagaatcgat tgcgcagctg tcatcacgct tcggcaagcc gttcgtggtg
     1921 gtcaactgcg ccgcagttcc cgagaacctt cttgagtccg aactcttcgg gtttgaaaag
     1981 ggttcattta ccggcgcagt gtctgcaaag gagggcatgc tgaagatcgc cgatcaggga
     2041 tgcgtcttct ttgacgagat tggagaactt ccgctctctc tgcaggggaa actcctccgc
     2101 tttctgcaga cacagacctt ttaccccata ggaagcacaa aggagattca ggtcaacgta
     2161 cgtgtcattt ctgctaccaa cagagacctg tccggcatgg tcagggaagg aaagtttcgg
     2221 gaggatctct atcacaggtt gcgcgttact tcgatccata tacccccgct gcgcgacagg
     2281 aaaaaagaca taccgccgct tgtccatttc tttctcaata agtacaaaca taccgcaccc
     2341 aggccgatca aagggataac aaaagaattc ttggggaaac tggtttccta tgattggcca
     2401 ggcaatatcc gcgagctcga aaataccata cgttctgcca tggccctgag caagacacac
     2461 tatctcacta cccatgaact gagggagctt gggagccatc ctgcttcgaa aaagactccc
     2521 tcctctgaaa cccttgcttc tgcgttagtg cctctcataa aagaagccat cgagaaaaag
     2581 gacaagaacc tctatgagag gatccacagc gaggtggata aaacagtgct tgagtatgta
     2641 ttgtcccgca caaaagaaaa ccagtcagag gctgcacgca ttctcggcat aaaccgttta
     2701 acgcttagga agaaactgag gttttaatct cgccggttaa taaatggccg aacccgccaa
     2761 aatcactgtc ctgagttgcc tggaatatgc acagggctaa ggtttatttt tccgtgtcgg
     2821 gttatgtatc ttatccatga tggaaaaaca ggtgctggta atcgcaacga gaaaccgaaa
     2881 aaaggttgag gagataagaa ggataatgga gggtgtggct gtggagctcc tgagtctcga
     2941 tgactttccg ggctgtcctg aagtggcaga ggatgcggat acttttgagg gaaatgccga
     3001 aaagaaggca agggcggtgg cgagctttac cggtaagccc tctgttgcgg atgattcagg
     3061 ccttgaggta tacgcgttga aaggcgctcc cggtgttctt tcagcacggt atgcggggac
     3121 cggggctgat gacaagaaga atcttggaaa gctccttgcc gaactgcagg ggacagaaga
     3181 gggcaggcgg ggaggccggt ttgtgtgcgt cattgccctt gcctttccca atggcagggt
     3241 ggaaaccttt cacggtgttg cggaaggaag gataggtacg gagccgaaag gatttaacgg
     3301 ttttggctat gaccctgtat tttatcccaa aggccatcac accacctttg cagagatgag
     3361 cgctgatgaa aaggatgccc tgagccaccg gggaatagca ctcagaaaat tgaggtccta
     3421 tctctcttct gtgacgtgac agtgaccttg tctgtcagca gtgttcatcc tctgctcatc
     3481 ttatgcacga ttctccgtct ctcacagcgt ttgtgaataa cgcggcttag tatttccgga
     3541 tcagttatag gaagataaaa gaaattaatc cctcaggtgc ccaatttttc ttgaaaaaag
     3601 ccggagggat gtcataaagt aacgtcttga ttcaacccta attaataaag gaggcattat
     3661 gaggcttgta ttacttggcg caccaggggc aggaaaggga actcaggcaa aaaagcttat
     3721 cgaaaaatat gcgattcccc aaatctccac aggggacctt ctccgtgctg cggttgcagc
     3781 aggaaccccc ctcggcaaag aggcgaaaac ctttatggac aagggcgagc tggtccctga
     3841 cagggttgtt ctcggtatgg tggaggagcg gctgaaacgg gatgactgta aaaagggata
     3901 tatccttgac ggattcccga gaaacacggc ccaggctgaa gcactggaca aaatgctcac
     3961 accactgggg atgtcgctga gcgcggcatt gagcgttgac gtgccctttg acgatctgat
     4021 gaagaggctc accggcagaa ggacatgcaa ggcatgcggc cagatgtaca acatttattc
     4081 aaacccgcca aaaaaggaag gaatctgtga caaatgcggg ggggcgttgt tccagaggga
     4141 cgatgataag gaagagacca taaaaaagag gctcgaagtg tataatgccc agaccgctcc
     4201 actcatggac tattaccgga agaaaggcat tcttagctcg gtcaagggta ccgggagtat
     4261 cgatgacatc tttcatgcgg tctgcaaatc ccttggtctg aaataaattt ccgggttgtg
     4321 ctctgttgcc cgttgcagta atcttcccac ctcccctcaa aatcagaggg gaggtgatgc
     4381 attcctcaac atgattataa agaaaactaa ttctccaatt ataattttaa aattgaattt
     4441 agtctgaact tggattaagg tagagtgccg ttgaattaat ccttagaaat agtctcgcga
     4501 aaaagtattc cttggaaggc aatttgggca ggaaagtcat cgggtcagcg gtgacggaaa
     4561 attattgtgg tgaccccagg cgttaaggga atcgaaaacc aaacaaagga ggaaaatgta
     4621 tggcgttagt aaatccgcat ggaaaagaga agaaattaaa acccctttta ttggtgggca
     4681 aggagttgga ggaagaaaag aaaaaagcta agacgctgaa acaagtcaag atatcgtcac
     4741 gcgaatcagg tgaccttatc atgatgggta tcggcgggtt tacccctctt gacggcttca
     4801 tgacttacga agactggaaa gatgtgtgtt ccgactacaa gatggccgac ggcaccttct
     4861 ggccgatacc tgtcaccgtt tctacggatg agaacatcaa ggcaggaaca gaagttgcgc
     4921 tctattccga cgagtacggc gaggtcatgg cgacaatgaa ggtttccgag gcctacaaga
     4981 ttgacaagga gttcgagtgc aagaacgttt acaggactac tgatatgaaa gagcacccgg
     5041 gtgtgaaaat ggtcatggac cagccgggca ccaaccttgc cggaccggtt aaggtcctga
     5101 gccagggaac attccctact gaatatccga acacctatat gacccctgca cagaccaggc
     5161 accttttcct tcagaacgga tggaaaaagg ttgcggcgct tcagctcagg aatcccatgc
     5221 accgttctca cgagtatctt gcaaagattg ctattgaaac ctgtgacgga gtgctcatcc
     5281 accagctcct gggcaagctg aaagccggcg atatcccggc agaagtgaga gtgcaggcga
     5341 tcgataagct ggttgagcac tattttgtaa agaacacatg catacaggcg ggctatccgc
     5401 tcgacatgcg atatgccggt ccgcgcgaag cgcttctgca tgcgcttttc aggcagaatt
     5461 acggctgcag ccacatgatt atcggccgcg accatgccgg tgttggcgag tattacggtc
     5521 cctttgatgc acaaaagata ttcgatgaga tccccgaagg cgcccttgaa acccagccgc
     5581 tcaagattga ctggaccttc tattgcacga aatgcgatgg aatggcatcg atgaggacat
     5641 gttgtcacgg caaggaagac aggatgctcc tgagcggaac caaattgaga aagatgctct
     5701 cggagaacga ggaagtgccc gaccacttca gccggccaga agtccttgca atattgagaa
     5761 aatactatgc aggactgacc gagaaggttg agataaagac acacaaatat tcggagggaa
     5821 taaaataagt tcggagtttt gagtaacggg ttcggagaga gtttctgctc cgaactcacc
     5881 actcatgatt tcagaagtga gaggaggtgg agatctagct catcggaaag ttttggatta
     5941 ctgccttaga tgctaaggct gatattgaga aacgtaaagg aggttaagga acatgccaag
     6001 tttcgtgatt accgagaagt gtgatggttg taaggctcag gataagaccg catgccagta
     6061 tatctgccct catgatttga tgaccctcga cagagaaaag atgaaggcat acaatcagga
     6121 gccagagcag tgctgggagt gctttaattg tgtaaagatt tgcccgacgc aggccatcga
     6181 ggtgaggggt tattcagact tcgttcccct cgggtccagc actatccctc tgagaggtac
     6241 cgactccatt atgtggacga tcaagttcag gaacggaata ctcaagaggt tcaagttccc
     6301 catcaggaca acagcagagg gttcggtgga cccgtatgca ggaaaaccgg agcctgattt
     6361 tgcaacactt aaacagcccg gtttgtttac cgccgcggca agaaaagaat aggaggtgaa
     6421 tatacatgga aaaagagacg tgtacatttt cattttgtaa gaagcccgag gtaaccgagg
     6481 ttgcatgtga tctcctgatc atgggcggcg gtatggcagg atgcggagcc gcattcgagg
     6541 catgccgttg ggcaaatgca aagggactga aggttgtcat ggtcgacaaa gcagcaacag
     6601 acagaagcgg cgcggtcgcg atgggtctgt cagccattaa tacctatgtc ggcgaaaata
     6661 aggttgcaga ttatgtgaaa tatgtgagga acgacctgat gggcatcatc agggaagacc
     6721 ttgtgtttga cctcggcagg ctcgttgacg attcagttca tctctttgaa gaatggggcc
     6781 tcccgatctg gaagaagggc gatgacggat tctcaataga tggtcatgag gcaaaagaaa
     6841 aaggcaagcc gatgctgaaa gacggcggaa caccggtacg gtccggtaaa tggcagatta
     6901 tgatcaatgg tgagtcatat aaggtcgttg ttgcagaggc tgcgaagaag gccctccaga
     6961 ccaacaggga aaagaccggc atggcacaga accattttga aagggttttg atcacaacac
     7021 cattgatgga tgtaaacggg aagaacgtag cgggagcggc gggctttagc gtgagagaga
     7081 ataaaatcta tgccttcaag gcaaaggcta tcatcaatgc tactgccggc gctgtgaatt
     7141 gcttcaggcc gaggtcggtc ggtgagggta tgggaagaac ctggtatcct gttatgagcg
     7201 ctggttcagg ttatgccttc ggaatgctgg ctggcgccga gctgacctta atggagaaca
     7261 ggttcgtgcc cgcaaggttc aaagacggct acggacctgt cggcgcatgg ttccttttct
     7321 ttaaggcaaa ggcaacagac agccttggga acgattactg tgcggataag gaatattttg
     7381 atgacgcggt agccagatac ggagattatg caaaaggtct cggtactgcg atcaggaacc
     7441 atctcatgat gagggcgatg agggacggca gaggcccgat ccttatgcag acccctgcgg
     7501 ctatggaggc cttaggtaaa gccttcaaag agaaacttgg cgataaggaa ggcgccaaga
     7561 agatgaaaca cctcgaggca gaggcgtggg aagacttcct cgacatggca atcggacagg
     7621 cgtcactctg ggcggcaagt aatgttgagc ccgataaggt tgtttccgaa ataatgccga
     7681 cagagccgta tctcctgggt tcacatgcag gctgcgcagg gttctgggta agcggccc
//
LOCUS       DATKOU010000042        16327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_441094, whole genome shotgun sequence.
ACCESSION   DATKOU010000042 DATKOU010000000
VERSION     DATKOU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 16327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16327
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441094"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VMU21_01765"
     CDS             <1..690
                     /locus_tag="VMU21_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO76280.1"
                     /translation="MGISGCGKSLFVKVIATRWRLPLIRLDMAVVYGGAFGSPESSLL
                     KAFRTVEAVAPCVLWIDEIESGISSQGFKAEGGAASRVLGSFLTWMQEKKAPVFVAAT
                     ANAIEMLPAEVLRKGRFDEIFYMGLPDETAREEIFRIHMKKQGIDPSGFDIPLLARSA
                     KRFSGAEIEQAVKSAAFEALADKRPLVEEDFLATLRATVPLAVTMNEQIISIEAWAFK
                     RAVPASGELRR"
     gene            716..1201
                     /locus_tag="VMU21_01770"
     CDS             716..1201
                     /locus_tag="VMU21_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76281.1"
                     /translation="MNRNKICPDCGTEYFPHIEKCADCGAVLLLPEELKKAQEEKKRL
                     MEKAVENEVVVREGDLSWLSELYAVLIDAGIPCTVRSDAGCKKSCCGDTCRLVVSPQQ
                     LERAQERIEEYFMEMHPEIRASRELAREGKCPACGSPVGPGDRECPDCGLPLVIIEEE
                     K"
     gene            1301..2296
                     /locus_tag="VMU21_01775"
     CDS             1301..2296
                     /locus_tag="VMU21_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HUO76282.1"
                     /translation="MYRIVRIASVLLLLVPAAFGTASDKPYSNPVLVETYPINRPKPA
                     RYVGTLGIGDPTVILYKEKYYLYPTGDNHSYHVYLSDDLVHWSKGPKVFQSNETGVWA
                     PDVFFNRDNGMFYLYYTVNGRIGVAEADRPDGEFRDLGNLISNGIDAHMFRDDDGSYF
                     LYYARYPEFGIFVQPMASPVLKKGEPVQVISPSEAWEMRNVPVTEAPWLLKHQGVYYL
                     LYSGGSANTEHYATGYATAKNPSGPFTKYPGNPIIRTEGGVLGPGHVSVTQDREGKLW
                     MVYHQQKDRTPGWNRIICIDPLWFDEKGVLHGKASRATPRAAPVTTGKVGETVRK"
     gene            complement(2355..5048)
                     /locus_tag="VMU21_01780"
     CDS             complement(2355..5048)
                     /locus_tag="VMU21_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HUO76283.1"
                     /translation="MPGRLPKKIVPVSLAVVLGLFFLPQFISLYVDWLWFNDVNFAKI
                     FTVRLNAQVMSALAGGLAGFVISYVSIWFSLQATKGRPVVMTFGNQAMPQLNVLRYFD
                     RLKIIIPILIGWVTGLSLKNNWLSFLYYFNRVDSGYSDPIFGKDVSFYLFTLPAYEII
                     SSLLMFILVASLALSVLTYILKGAVFMTQRGIISERSASAHLSVLGSLIFLVLAFKTY
                     TGMYTILSSSHGHVAGATYTDVHAVIPFLKARIVIALAAAVLLISNIFLRRTLIFIGT
                     AVLYLAVSFIGSSVYPAIIQKFVVAPNELVKETPYITHNIASTRKGFGLDRVEERDIS
                     GSTTITRDDITKNSPTIKNIRLWDHRPLLDTFSQIQEIRTYYDFTMVGNDRYVINGEY
                     RQTMLSPRELSTESIPTRNWINETLSFTHGYGLTLGPVNQVTPEGLPVLLIKDIPPVS
                     TVADIKVTRPQIYYGKLSSNYVIVNTKSKEFDYPSGEANVFTEYSGKSGVVIDSFFKK
                     FAFSVHFASMKLFLSNDITGKSRILFNRNILERVSKVMPFLKFDHDPYMVVSDDGQLY
                     WIYDAYAVSGRFPYSQLVGNGINYIRNSVKVTINAYDGSMKFYIADKDDPIIRTIHRM
                     FPGTLQPVSEMPADLKKHIRYPLDIFGIQTMVYSTYHMDVPQIFYNKEDQWEIPAMGK
                     SEELMEPYYTIMKLPGEKKEEFILMLPFTPKKKDNLSAWMVARSDGEHYGKLVVYRFP
                     KDRLVYGPKQIVARINQDTEISRQVSLWDQRGSQVIQGTLLVIPIENSLIYVQPLYLR
                     ADKGKIPELKRVVIAYENRIAMEETLDAALSKIFGDVRVSEEAAPSKSLRLPAVKEEK
                     GLASGAREHFDRAMNAQREGNWALYGEEIKKLGEIIRKLQK"
     gene            5291..5779
                     /locus_tag="VMU21_01785"
     CDS             5291..5779
                     /locus_tag="VMU21_01785"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HUO76284.1"
                     /translation="MTTNTDIHRWWVRKWLLLCVLIVGTFITCASAEDAYWVVFGKSN
                     IGTAYYDKAGIEQVSADVIRVSVKYSYSSEGIREFREAFPEVNAHETVGYSLYLYEIS
                     CSSGSFVLLKAATYNPAGNVLKGTELLLNGNENPTPQHITPHSLMEQLSDAACKWKLN
                     DR"
     gene            6272..6883
                     /locus_tag="VMU21_01790"
     CDS             6272..6883
                     /locus_tag="VMU21_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HUO76285.1"
                     /translation="MLHFLDLFGVAVFAVTGALAAGKKKMDLFGVVVLAVATALGGGT
                     LRDVILGAYPVFWISNPTYIFVGTAAAVSIFIMARFMRPPSKALTFADAFGLAIFTVI
                     GTEKAMSLGVPGSVAAIMGVMTGVAGGIIRDMLSGEIPLILKAEIYATASLCGAITFA
                     LLTRHFPAMLQMPALVAITVILVLRLAAIHWNLSLPIFPPDKR"
     gene            complement(7351..7671)
                     /locus_tag="VMU21_01795"
     CDS             complement(7351..7671)
                     /locus_tag="VMU21_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76286.1"
                     /translation="MTGPLKLLFDRNVPVFEYYELYTWRFPQPKHKGKPAAIVTASLS
                     PFPFNYLPSQGSGALRAVKIVLNAAGFAIRGQIIVPMTRDLAHIGKRWLAKARKLGKK
                     IGSW"
     gene            complement(7681..7935)
                     /locus_tag="VMU21_01800"
     CDS             complement(7681..7935)
                     /locus_tag="VMU21_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HUO76287.1"
                     /translation="MILFLNASPRKNGVTSTLLKIIAGEARSAGAEVEWVDVNSLSTR
                     PCIGCLKCRPDKKCILPKDDAHRIGELIERCSGLVVGTPT"
     gene            complement(8353..9129)
                     /locus_tag="VMU21_01805"
     CDS             complement(8353..9129)
                     /locus_tag="VMU21_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76288.1"
                     /translation="MDLFIEMINRFKPEYAIGFPQTIAAFSSYVKRKKRILGGCFKGI
                     ILIGEQVYAWQKNLIEGVMGARVFLDYGMVEKAIHGGGCEHADAYHFIPQYGLTEYVP
                     LQNHFNELVGTGFINYAMPLIRYRTGDICRHIRKGCTVCGRPYDTTAAIDGRLCDFLV
                     TADGQIVSVHLPLDHRVLKHIEGFQLYQEKAGEAELRVWTRRKEGKHAGRLLHEIQRC
                     TSPLGRDLVYKIVAMGNEHTRTQTKYSVVDQQLDMRNFLQ"
     gene            complement(9307..9756)
                     /locus_tag="VMU21_01810"
     CDS             complement(9307..9756)
                     /locus_tag="VMU21_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76289.1"
                     /translation="MGTIEIIRKFIESNRVIERAVREGVERLPLRFRYGISYGPTFRY
                     WLAFLKESENWDRERLEAYQVEQLRDLLMHAGKTVPYYRRVFREYGFKPEEIQILDDI
                     RVLPYLDKTIVKTNRPEFIATNIPKKRLIAVTTSGTTGIPLSMYGTK"
     gene            complement(9787..10512)
                     /gene="pgl"
                     /locus_tag="VMU21_01815"
     CDS             complement(9787..10512)
                     /gene="pgl"
                     /locus_tag="VMU21_01815"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HUO76290.1"
                     /translation="MMPEYLVFDTSDEIWHYLGKSWKEISAKAIGENGYFCAALSGGK
                     TPTGFYQYLSGKHLQWEKSHLFLVDERFVPWDSPESNYRMLQENLLGRIQIPRENIHP
                     ISTNQSSPEISARTYEEELRRFFKLDENGIPEFDLVLLGIGEDGHTASLFPGTAAVKE
                     TKRLLVSVSPGNALMDRISLTLPVINNAWNVIFLVTGQRKRAVIKKIREGRDTTLPAS
                     MINPKRGRLLFLMDKEAAGTRRL"
     gene            complement(10509..12059)
                     /gene="zwf"
                     /locus_tag="VMU21_01820"
     CDS             complement(10509..12059)
                     /gene="zwf"
                     /locus_tag="VMU21_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HUO76291.1"
                     /translation="MDTGGREPVFSKFLQNCDIPLAPQHIGPFTMVIFGGGGDLSRRK
                     LLPTLFHLYRENELPAHFSLIGFGRHDMADGPYRALARESVTEFGEEPFDENGWNEFS
                     THLFFAPGRFEDDESYKKLIEKVSRVSIPSEEGKMEVIYYLAVPPHATPVIVEKLGAH
                     SLSQGKFNTRIIVEKPFGRDLPSAAKLNRALTQAFDERQIYRIDHYLGKETVQNIIFF
                     RFANAVFERLWNSRYVDHVQITVAEDMGIEHRGAFYEETGVIRDIVQNHMMQLMALVA
                     MEPPIGFGADLIRDEKVKALRAIKPMSNEEITKFTVCGQYGPGKLRDLQVAGYREEKG
                     VSPESLTPTFFGGRFYIANWRWAGVPFYVRTGKRLARRVTEICIQFTQPPLRLFGRTC
                     DVLEPNVLSLTLQPEEKISLKFGVKYPHVNNKIYSATMAFSYQEAFGLKAHPAYERLL
                     LDCMKGDLTLFVRQDDIEATWEVIDPITALWEDIRRWDLPLYPAGSWGPEGSDLLLSQ
                     DGRTWLTE"
     gene            complement(12063..12974)
                     /gene="gnd"
                     /locus_tag="VMU21_01825"
     CDS             complement(12063..12974)
                     /gene="gnd"
                     /locus_tag="VMU21_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HUO76292.1"
                     /translation="MKLGFVGLGKMGGNMVKRLLERGHEIIAFAPSETAREEAKAHGA
                     QVVDSLQGLAEKVAPPRVVWLMVPAGRATDEVIQSLAPLLTRGDILIDGGNSFYKDSM
                     ARAEFLRTKGIFFLDAGTSGGIWGLKEGYCLMVGGEEDAFKKAEPIFRDLAPRDGYSH
                     MGPSGSGHFVKMVHNAIEYGLLQAYGEGFELMHAKKEFSLDLRKIAELWNHGSVVRSW
                     LLELSVNAFSQDPSLQSVKGYIEDTGEGRWSVLEAIQENVPAPVITLSLLQRLRSRQE
                     ESFSAKVIAALRGQFGGHEVKKEKGKG"
     gene            13151..13345
                     /locus_tag="VMU21_01830"
     CDS             13151..13345
                     /locus_tag="VMU21_01830"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HUO76293.1"
                     /translation="MKASVRMTSIRLDTKLADDAVKALGAKNRSEAVHIALREVVALK
                     KFKELMVKHGGKLKFEAHGN"
     gene            13335..13745
                     /locus_tag="VMU21_01835"
     CDS             13335..13745
                     /locus_tag="VMU21_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO76294.1"
                     /translation="MATRIIIFDTSVFIDHLRTNKYSDRLKDLSGLIRNSSVVLSELA
                     RGATDKTELDFVSALEKNHPVLTPMERNWIESGHILHRIYKDKGFLPEKLRDLHFDVL
                     IALTARNHGATVITSNMTDFELIRRYKEFRLEIW"
     gene            complement(14151..14645)
                     /locus_tag="VMU21_01840"
     CDS             complement(14151..14645)
                     /locus_tag="VMU21_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76295.1"
                     /translation="MNKKLSILLGILVCVGGLSILIFWNAIADKAQTLVLFVTLIIIT
                     VYVIDTHRIANSAAMQSRIYWISNLFVSLSVRTDGFIKDIEGPSLFPEQWAVHTREMR
                     SLLEVLLHIWPSTSGRDHINAVLEECSTRFQTSPMMDQDKADLISFLLRVKRELQIMV
                     GQLS"
     gene            complement(14786..15922)
                     /gene="tal"
                     /locus_tag="VMU21_01845"
     CDS             complement(14786..15922)
                     /gene="tal"
                     /locus_tag="VMU21_01845"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HUO76296.1"
                     /translation="MAANPLKALNEAGQCVWLDNLYRDLITTGSLMHMMEDGITGITS
                     NPTIFQKAISGSAAYDESIIMMLRKGITDPKELFLGLAMEDVSRAADVLRPVYGTSKC
                     LSGYVSIEVSPDLAHDTRGTIKEALHLFSAINKKNILVKVPATKEGLPAIEELTASGV
                     NVNVTLLFSIERYKEVAEAYIRGLEQRLRKKEQINEIISVASFFVSRVDTLVDKLLEQ
                     RIASSSGEEKNRLTALLGKAAVANAKLAYREYERIFTTSRFLSLKEKGANVQRLLWGS
                     TGTKNPKYSDIKYVDELIGPDTINTMPEATLNAFKDHGTVKITIGEQMDKAEKVLKEL
                     ASLGISIKEVTDQLEKDGVKAFADSYFALLDEISKRRDRIQAEK"
BASE COUNT         3867 a         4171 c         4074 g         4215 t
ORIGIN      
        1 atggggatat ccggctgcgg caaatcgctg tttgtcaagg tgatcgccac tcggtggcgc
       61 cttcccctta tccgcctgga tatggcggtt gtgtatgggg gggcgttcgg ctccccggag
      121 tccagcctgc tcaaggcgtt caggaccgta gaggcggttg cgccctgtgt cctctggata
      181 gatgagatcg agtccggcat ttcatcgcag ggtttcaagg ctgaaggagg cgccgcttcc
      241 cgggtcctcg ggtctttcct gacgtggatg caggaaaaga aggcgcctgt ttttgtcgct
      301 gctaccgcca acgccatcga aatgcttccc gcggaagtcc tgcgcaaggg acgcttcgat
      361 gagatatttt atatggggct tcccgatgaa acggcgcggg aagaaatatt ccgtattcat
      421 atgaagaagc agggtatcga tccttccggg tttgatatcc cgctgctggc gcgatcggcc
      481 aaacgttttt ccggagctga aatcgagcag gcggtcaaat cagcggcttt cgaggccctt
      541 gccgataagc gtcccttagt cgaagaggat ttcttggcaa ctctgagagc gaccgtccct
      601 cttgcggtga ccatgaatga acagataata agcatcgagg cctgggcctt taagcgcgca
      661 gtgccggcct cgggagaatt aaggcgttag cggaaatcac aggctgtcac gcactatgaa
      721 ccggaacaag atatgtcctg attgcggaac cgagtacttc ccgcacatcg agaaatgcgc
      781 ggattgcggc gcagtcctcc tcttgcccga ggagctcaaa aaagcacagg aggaaaagaa
      841 acggctcatg gagaaggccg tagagaacga ggtcgtggtc agggagggtg atctcagttg
      901 gctgagtgag ctgtacgcag tcctcatcga cgccggtatc ccctgcaccg tccgttccga
      961 cgcaggctgc aagaaaagct gctgcggtga tacatgccgt ttggtcgtat caccgcagca
     1021 gcttgaaagg gcgcaggagc ggattgagga atacttcatg gaaatgcatc ccgagatccg
     1081 cgcttcccgc gaactggcca gagagggcaa atgcccggcc tgtggttccc ctgttggccc
     1141 cggcgaccgc gagtgccccg actgcggcct gcctctcgtc attattgagg aggagaaatg
     1201 aatagggaca gacacacgtg caatagcgca gaagtgcaaa agttcagaaa tccgaacatg
     1261 aaagtgtaaa aggggaaagg ccgttacggt ataataatgg atgtacagga tcgtgcggat
     1321 cgcgtcggtg ctgctcctat tagtccctgc agcgttcggt acggcatctg acaagcccta
     1381 ttccaatccc gtcctggtcg aaacctaccc gattaatcgg ccaaagcccg cgcgatacgt
     1441 cgggacgctc ggtataggcg atcccaccgt tattctttac aaggaaaaat attatctcta
     1501 cccgactggg gacaaccaca gctaccatgt ctacctgtcc gacgacctgg tccactggtc
     1561 caaggggccg aaggtctttc agagcaacga gaccggtgta tgggcgcctg acgtcttctt
     1621 caaccgggat aacggtatgt tttatcttta ctacacggtg aacggcagga tcggcgttgc
     1681 ggaagcagac cgtcccgacg gggagttcag ggacctgggg aacctgatca gcaacgggat
     1741 agatgcgcac atgtttcgcg acgacgacgg ctcatacttc ctctattacg cacggtaccc
     1801 ggaattcggg atatttgtcc agcccatggc gagccctgtc ctgaaaaagg gtgagcctgt
     1861 tcaggtcatc tctccttccg aggcatggga aatgagaaat gtgccggtta ccgaggcccc
     1921 atggctgctc aaacaccagg gcgtctatta cctcctttac tccgggggca gtgcgaatac
     1981 agagcattac gccaccgggt acgcaacggc aaagaaccct tcaggtccct ttaccaagta
     2041 tcccggaaat ccgatcatcc gtacggaggg tggcgtgctg gggcccgggc atgtgagtgt
     2101 cacacaggac cgggagggga aactctggat ggtctatcac cagcagaagg ataggacacc
     2161 gggctggaac aggataatct gcatcgaccc tctctggttc gacgagaagg gtgtcctcca
     2221 tgggaaggcc tcacgggcga cgccccgcgc cgcgcctgtg accacaggga aggtcgggga
     2281 aacggttcgg aaataaatcc cgctgactct ctgtccatca ggaagaagca gaacagggct
     2341 tttcagcccg cctgtcattt ctgcagcttc ctgattatct cccccagctt cttgatttct
     2401 tctccgtata aggcccagtt cccctccctc tgggcattca tggcgcggtc aaaatgctcc
     2461 ctggcacctg aagcgagacc cttttcctcc ttcaccgcgg gaagccgcag ggacttcgag
     2521 ggcgccgcct cttccgagac cctgacatct ccgaatattt tcgaaagggc cgcgtcgagt
     2581 gtctcctcca tggcgatacg gttttcgtat gcgatgacga cccttttcag ttcaggtatc
     2641 ttgcccttat ctgccctgag ataaagcggc tgaacatata tcagggaatt ttcgatcggt
     2701 atcaccagca gggtcccctg gatcacttgt gagccgcgct ggtcccagag agatacctgc
     2761 ctcgatatct cggtatcctg attgatcctg gcgactatct gcttcggccc gtatacgagc
     2821 ctgtccttcg gaaagcggta taccacgagc ttcccgtagt gttcgccgtc actgcgtgcc
     2881 accatccagg cagagaggtt gtcctttttc ttcggggtaa aggggagcat caggataaac
     2941 tcctccttct tctctccagg aagcttcatt atcgtgtaat agggttccat aagctcttcg
     3001 ctttttccca ttgccggtat ctcccactgg tcttccttgt tgtaaaagat ctgtgggacg
     3061 tccatgtgat aggtcgaata gaccatggtc tgaataccga aaatatccag gggatacctg
     3121 atatgtttct tcaggtcagc cggcatctct gacacgggct gaagcgtgcc ggggaacatc
     3181 ctgtgtatcg tcctgataat ggggtcatct ttgtctgcaa tataaaactt catactgccg
     3241 tcgtacgcgt tgatcgtcac cttcaccgag ttccttatgt aattaatccc gtttccgacc
     3301 agctgtgaat aggggaatct tccgctcact gcgtatgcgt cgtatatcca gtaaagctgt
     3361 ccgtcatcag acacgaccat gtacgggtcg tggtcaaact taagaaaagg catgaccttg
     3421 ctgacccttt caaggatatt cctgttaaac aggatcctgc ttttaccggt tatgtcattt
     3481 gagagaaaga gcttcatcga ggcgaaatga accgagaagg cgaatttttt gaagaatgaa
     3541 tctatgacca ccccgctttt gcccgaatat tccgtaaaga cattcgcctc tccagacgga
     3601 taatcgaact cttttgactt tgtattcacg atcacgtagt tgctggacag cttgccgtaa
     3661 tagatttgag gcctggtgac ctttatgtcc gccacggtcg aaaccggggg gatgtcctta
     3721 atgagcaata ccgggagtcc ttcgggtgtc acctggttta ccggcccgag cgtcaggccg
     3781 tatccatggg taaaggacag ggtctcgttt atccagtttc gcgtcgggat gctttccgta
     3841 gacagctctc tgggtgaaag cattgtctgg cgatattccc cgttgatcac ataacggtca
     3901 ttgccgacca tagtgaagtc gtaataggtg cgtatctcct ggatctggct gaaagtatca
     3961 agcagtggtc tgtggtccca cagccggata ttttttatgg ttgggctgtt ttttgtgata
     4021 tcatccctgg tgatcgtggt gctgcctgat atgtccctct cttccactct gtcaaggccg
     4081 aagccctttc ttgttgaagc tatattatgc gtgatatagg gggtttcttt taccagttca
     4141 ttcggcgcca ccacaaattt ctggatgatc gcagggtaca ccgaactgcc gataaaagac
     4201 actgcgagat agagcactgc agtcccgata aagatcagag tgcgtctcag gaaaatattg
     4261 cttatcagaa ggactgccgc agcgagcgcg ataactatcc ttgccttcag aaaggggatc
     4321 accgcatgaa cgtccgtgta ggtcgcgcct gccacatgac catgcgaaga actcaggatg
     4381 gtgtacatcc ctgtatatgt cttgaacgcg agtacaagga aaataagaga ccccagcaca
     4441 gagagatgtg ccgaggcaga gcgttcagag attatgcctc tctgtgtcat gaacaccgcg
     4501 cccttcagta tataggtcaa cactgagagc gcaagggacg ccaccaggat gaacatcaga
     4561 agtgaagata tgatctcata tgcagggaga gtaaacaggt aaaaagagac gtctttgccg
     4621 aatatcggat cagaatagcc ggaatcgacg cgattaaaat aatagaggaa gctcagccag
     4681 ttgttcttta aagataatcc tgtaacccat ccgatgagta tcggtataat gatcttcagg
     4741 cggtcaaaat atctcaggac attcagctgg ggcattgcct gattaccaaa cgtcatgaca
     4801 acaggcctgc cttttgttgc ctggagggaa aaccagatgc ttacatacga gatgacaaaa
     4861 cctgccagcc cgcctgccaa cgcgctcatt acctgggcat ttaacctgac agtgaatatt
     4921 tttgcgaaat tgacatcgtt aaaccacagc cagtcaacat acaatgaaat gaactgcggc
     4981 agaaaaaata aaccaagaac cactgcgagt gagaccggca ctatcttttt tggtaatctt
     5041 ccgggcatat aacccccctt atcggtattc tcggtatata aagatatatt accactaatt
     5101 cacagcccag gcatcaacgg tctgcgggtc catccgtacg gctcggctcc aaatatcggg
     5161 acctggcatg ctttgcctct ataatttata ctatagttca tgaggatagt tgcgtgaagt
     5221 ttttttgttt tctgttgaac acctgttcgc tgggatgcga aagtgaactt acaacagaaa
     5281 ggggtgcaga gtgacaacaa acactgacat acaccggtgg tgggtcagga aatggcttct
     5341 tttgtgtgta ctgattgtcg gtacatttat cacatgtgcc tcagcagaag atgcctattg
     5401 ggttgttttt gggaaaagta acatagggac cgcatattat gacaaggcag gtatcgaaca
     5461 ggtttcggct gatgtcatca gggtctcggt caagtactca tactcatccg aggggataag
     5521 agaattcagg gaggcattcc ctgaggtgaa tgcgcatgag acagtcggtt attccctgta
     5581 cctgtacgaa ataagttgct cctccggctc ttttgtgctg ctcaaggccg cgacctataa
     5641 tccggcaggc aacgtactca agggcacaga actgcttctg aatggaaatg agaatccgac
     5701 cccgcagcat atcactcctc attcactcat ggagcagttg tccgatgcgg cgtgcaaatg
     5761 gaaactgaat gaccgctgac gttctgtcaa gtagttgtca gagggaaaac ttaaggctgt
     5821 agcaggaggg tgggaccctt cggacctgaa atttctgaag accgacaaga ttacgtttag
     5881 cgtccctatg aaatgttcag gcgtgtgatc atccgttggc ccgaatcgtt tttgagtacc
     5941 aagcctggtc aatagtaaag aagaaaatag aactgagcaa gaaggcctgg ggagaaaccc
     6001 cgaagggata atatattcct tgtcgggaac cccacgcatg tggtcctccg aataacacat
     6061 gataaccaac actgcggagt tcttttcaag atcctcgatt tccgttgcta acccacggtt
     6121 ttatgccgat aagcagggtt gtgaaccctc agacgatgtc tcccatttcc cttgaccaac
     6181 aaagttaaat gcacgtcttg ctttgaaagc cgtaacgtaa tattatgttt ttagtggaat
     6241 tatttttgct gggacagagg tgggaggttt gatgcttcat tttctcgacc tgttcggcgt
     6301 tgcagtattt gctgtcaccg gcgcactggc tgctggaaag aaaaagatgg acctcttcgg
     6361 agtggtcgta ctcgcggttg cgaccgcact cggcgggggg accttgagag acgttatcct
     6421 cggggcgtac cccgtcttct ggatttccaa tcctacgtat atctttgtag ggacagccgc
     6481 tgccgtttcg atattcatta tggccaggtt tatgcgaccc ccgtcaaagg cactcacgtt
     6541 cgccgatgca ttcgggttag cgatcttcac agtaatcgga accgagaagg caatgtctct
     6601 tggagtgccg ggctctgttg ccgcaatcat gggagttatg accggtgttg ccggcgggat
     6661 tatcagagat atgctttcag gcgagatccc gctgatcctg aaggccgaga tatatgcgac
     6721 cgcttccctc tgtggggcca tcacgtttgc cttactcaca cgacattttc ccgcaatgct
     6781 gcagatgccc gcgcttgttg cgatcaccgt gatcctggtt ctcaggctgg ccgcgattca
     6841 ttggaatctc tcactcccga tttttccacc ggataaaaga tgatgcctca ggttgaggca
     6901 gtccttccac gatagttagt ccacctcctc agttattgtg acccgcaact gacaaggact
     6961 ttgtcctgta ccgcgcacaa aaaaatcgga atgaaaatcc atgccagttc ccctcctgat
     7021 ctcccagcgg attattggcg gtgttgcttt ctttcggtat tcaacggact atggcttaat
     7081 tactcgttaa agatacttat atcccgtagt acgcacgtcc actgacatgt gaataaagta
     7141 gtcctgcgat ctcggttcca tcgacaacat cacacaatta aaatctttgc aacaattacg
     7201 ttttacggaa ggaactggta ggaagaaacg tgaagctcac ctggagcgct ggtgaaccgg
     7261 tggaacgctg tgtcaggctg atggcggttc gtcctgcccc tacccagcga gcccctcaag
     7321 tgtgctggac aggtcgagcg gctatcatta ctaccaggat cctatcttct ttcctagttt
     7381 tctggccttt gccagccagc gtttcccgat gtgtgcgagg tctcgggtca tcgggacgat
     7441 aatctgtccc ctgatggcga agcccgcagc gttcagcacg atcttgactg cgcggagagc
     7501 cccactgcct tgcgaaggaa ggtagttaaa gggaaacggc gacagagagg cggtaacgat
     7561 cgctgccggc ttgcccttat gcttcggttg tggaaagcgc caagtataga gttcatagta
     7621 ctcaaagaca ggaacatttc ggtcaaagag aagctttagc ggcccggtca tgttgcccca
     7681 ctaagtaggg gtgcccacca ccaaaccgga gcagcgttct atcagctctc ctatacggtg
     7741 cgcgtcgtct ttgggaagaa tacacttttt gtcgggcctg catttcaggc acccgataca
     7801 tggccttgta gaaagactgt tgacgtcaac ccactccact tcggcaccgg cagagcgggc
     7861 ttctccggca attatcttga gtagcgtcga tgtcacacca ttcttcctgg ggctagcatt
     7921 aagaaagagg atcattctcg ccacctcctc ttttatattc tacccgtcct tacccaacga
     7981 ttaccagtta tcaaaagtaa ctcacagcga ctgaacgttg aggtcgccgt tacttacagg
     8041 aaacagtcaa aaacgatttc ttacctttgt gagggacgtt ccttatgcga agtacgtgat
     8101 aagatcttcc ttcacaagaa atagctgggc tcggcaacaa tgcttcctga agtaaacagc
     8161 tatgatgaag ttcttctaac tgaatctcac atacggtaac caatagggca agtcatcttt
     8221 ttgaacgttg aggttagaaa tctaattacc atgaaaaagt tacgatacat tattgacgcg
     8281 cacaccatgg ccgataagga tagcaatacg gggtttagca gtgaaatacg cagggacaca
     8341 tcaaccaaat acttactgca agaagttcct catatccagt tgttgatcaa cgacgctgta
     8401 ctttgtctga gttcttgtat gctcatttcc cattgccacg atcttgtata caaggtccct
     8461 cccaagggga gaggtgcagc gctgtatctc atgcaacaat ctgccggcat gctttccctc
     8521 tttgcgtctc gtccatacac ggagctccgc ctcaccagcc ttttcctggt agagctgaaa
     8581 cccctctata tgcttcaaca cacggtgatc cagagggaga tgtaccgaaa caatctgtcc
     8641 gtcggcagtg acgagaaaat cgcagagacg gccatctatg gcagcggtgg tatcatatgg
     8701 tcttccgcac accgtacacc ccttccgtat atgtctgcat atgtcacctg ttctgtatct
     8761 gatgagcggc atagcataat tgataaatcc cgtgccgacc aattcattga aatgattctg
     8821 taacggcacg tattccgtga gcccatattg agggatgaag tggtatgcat cagcatgctc
     8881 gcatcctcct ccgtgaatcg ccttctccac cattccgtaa tctaaaaaga cccgtgcccc
     8941 cataacgcct tcaataagat tcttctgcca tgcatagacc tgctcaccaa tgaggataat
     9001 gcctttaaag cacccgccca gaattcgttt cttgcgttta acgtaggaag agaaagcggc
     9061 aatcgtttgg ggaaagccta tcgcatattc aggcttgaac ctgttgatca tttctataaa
     9121 cagatccatc catgggtcct taaggtaatt actcgagata ataagcttat tcccgtactt
     9181 cttccaaggg aagggctctt ccccgccctc cctcgcagta ctctcaaaaa ggaccgttct
     9241 tgatcctgga ctgtatccta ttttattcca tagatgaaca accgttgccc aatgtttctc
     9301 ctcggtctac tttgttccgt acatgctgag gggaatccct gtggtgccac ttgtcgttac
     9361 cgcgatcaat ctttttttcg gtatgtttgt cgcgataaac tcagggcgat ttgttttcac
     9421 aatcgtcttg tccagatatg gcagaacccg tatgtcatca aggatctgta tctcctcggg
     9481 cttgaatcca tattccctga acaccctccg gtaatagggc acagtcttcc ccgcgtgcat
     9541 caataaatcc ctcagctgct caacctgata tgcttcaagc ctttccctat cccagttctc
     9601 cgactccttc aggaaggcta gccagtatct gaaggtcgga ccataggaga tgccgtagcg
     9661 gaacctcaag ggaagtcttt ctaccccctc cctcaccgcg cgctcaatta cgcgattgga
     9721 ttctatgaac ttcctaatga tttctatggt tcccatgagc gtgaatcggg agcgcctctc
     9781 ccttcctcac aatctgcgcg tgcccgctgc ctctttgtcc atcaggaata agagccttcc
     9841 cctcttagga ttaatcattg acgcggggag tgttgtatcg cgtccttctc tgattttttt
     9901 tattactgct ctctttctct gtccggtcac gaggaagatc acattccatg cgttattgat
     9961 caccggtagg gtgaggctta ttctgtccat gagggcattt cccggactta ccgagacgag
    10021 aagacgtttt gtctccttta ctgcagcagt gccagggaaa agtgatgcgg tatggccgtc
    10081 ttccccaatg ccaaggagaa caaggtcgaa ttcaggaatc ccattttcat caagcttgaa
    10141 gaatcgtctc agctcctctt catacgttct tgcagatatt tcgggagaag actgattggt
    10201 tgatatagga tgaatattct ctcggggaat ctgtatcctg ccgaggaggt tctcctggag
    10261 catcctgtaa ttgctttcag ggctgtccca tgggacaaac cgctcatcaa caaggaagag
    10321 gtggctcttt tcccattgca gatgttttcc cgaaagatac tggtaaaagc ctgtcggggt
    10381 cttgcctccc gagagtgctg cgcaaaagta tccgttctct cctatcgctt ttgccgaaat
    10441 ctctttccag cttttcccca aatagtgcca aatctcatcg ctggtatcga agaccagata
    10501 ctcgggcatc attctgtgag ccaggtgcgg ccgtcctgcg aaaggaggag gtctgagccc
    10561 tcagggcccc atgaacctgc gggatagagc ggaaggtccc agcgtcttat gtcctcccag
    10621 agggctgtta tgggatctat cacctcccac gttgcctcaa tgtcatcctg tcttacgaag
    10681 agggtaagat ctcctttcat gcaatcgaga aggagccgtt cataggcagg gtgggccttg
    10741 aggccgaatg cctcctggta gctgaaggcc atggtggcgg aatatatctt attgttgaca
    10801 tgggggtatt tgaccccgaa tttgagcgaa atcttttctt caggctgcag ggtgagggac
    10861 agtacattcg gttcaaggac atcgcaggta cggccgaaaa gcctgagcgg aggctgggtg
    10921 aactggatgc atatctcggt gacacgtcgt gcgagacgct ttcctgtgcg cacataaaag
    10981 gggacccctg cccacctcca gtttgcgata taaaatcttc ctccaaagaa ggtaggggtg
    11041 agggattctg gagaaacgcc cttctcttcc ctgtaccctg ccacctgcag gtcgcgcagt
    11101 ttccctggtc cgtactggcc gcagacagta aatttcgtga tctcttcatt gctcatgggc
    11161 ttgatggcgc gcagcgcctt caccttctcg tcgcggatga ggtcagcccc aaagccgatg
    11221 ggaggttcca tggcaaccaa agccatgagc tgcatcatgt ggttctgcac aatgtccctt
    11281 atcaccccgg tctcttcata gaatgcgccc ctgtgctcaa tgcccatgtc ctcggcaacg
    11341 gtaatttgta catgatccac ataccggctg ttccagagcc tttcgaacac tgcgttggca
    11401 aaacggaaga agatgatgtt ctgaaccgtc tccttgccaa gataatgatc gatgcggtag
    11461 atctgtcttt cgtcaaaggc ctgggtcagc gcacggttca gcttcgcggc agaggggagg
    11521 tcccgtccaa aaggcttttc cacgatgatc ctggtattga atttcccttg tgacaggcta
    11581 tgtgccccca gtttctccac aatgaccggc gtggcgtggg gcggtactgc aaggtaataa
    11641 atgacctcca tctttccctc ttcagaaggg atcgaaaccc gggagacctt ctcgatgagt
    11701 tttttgtagc tttcgtcatc ttcgaaacgg cctggtgcga aaaagaggtg tgtgctgaat
    11761 tcgttccacc cgttttcgtc aaagggttct tccccgaact ctgtgaccga ttccctggca
    11821 agggcgcggt aaggtccatc agccatgtcg tgtctgccga accctatgag ggagaaatgg
    11881 gcaggtagtt cattctctct gtaaagatgg aagagggtgg ggaggagttt cctcctgctc
    11941 aggtcgccac ccccgccaaa gatgaccatg gtaaaaggcc caatgtgttg gggcgcaagg
    12001 gggatgtcgc agttctgaag gaatttactg aagaccggtt cgcggccgcc tgtatccatt
    12061 tatcaacctt ttcccttctc cttttttacc tcgtgtcccc cgaactggcc cctgagggct
    12121 gcaatgacct ttgcgctgaa ggattcttcc tgcctggagc ggagcctctg gagaagggag
    12181 agggttatca caggcgcggg gacattctcc tggatggcct caagcaccga ccatctccct
    12241 tctccggtat cctctatata gcccttcact gactgaaggg aggggtcctg gctgaatgca
    12301 tttaccgaaa gttcgaggag ccatgaccgc accacgcttc catggttcca gagttctgct
    12361 atttttctga gatcgaggct gaattccttt tttgcgtgca tgagttcaaa accctcgccg
    12421 tatgcctgga ggagtccgta ctcgatggca ttgtgcacca tctttacgaa gtggccagaa
    12481 ccactgggac ccatatggct atacccgtct ctgggagcga ggtccctgaa tatgggctct
    12541 gccttcttaa acgcatcttc ttctccaccc accataaggc agtagccctc tttcaggccc
    12601 catattccgc cgcttgtacc cgcgtccaga aagaagatgc ctttggtcct gaggaattcg
    12661 gcccttgcca tggaatcctt ataaaaggag ttgcctccgt cgatgaggat atcacctcgg
    12721 gtgagaaggg gcgccagtga ttggatcacc tcatcagtag cgcggccggc agggaccatc
    12781 agccatacca cacggggagg ggccaccttt tccgcaagcc cctgcaggga gtcgaccacc
    12841 tgagcgccgt gtgccttggc ctcttcccga gccgtttctg aaggagcaaa ggcaataatc
    12901 tcatggcccc tctcgaggag acgcttcacc atgttccccc ccatttttcc aagacctaca
    12961 aaaccgagct tcatcctatg acctcctatt ctgtttgact ctgtattcat ccatacaaag
    13021 agattaccag aatattgggg ggaagttaag accttatagg gacagctaat tcccatcacg
    13081 aatactctct tgacaaatgt atatagaaat aatactttat gtatatacaa agcaaatgga
    13141 ggtttaagtc atgaaggctt cggtgagaat gacatcaata aggcttgata caaagcttgc
    13201 ggatgacgca gtgaaagccc tcggcgcaaa aaacagaagc gaagcggtgc acatcgcgtt
    13261 aagggaggtc gtggcgctta agaagttcaa agaattgatg gtaaagcacg gcgggaaatt
    13321 gaaatttgaa gcccatggca actaggatca ttatttttga tacctcggtc tttattgatc
    13381 atctgagaac gaataagtat tccgatcggt taaaggatct gagtggcctt ataagaaact
    13441 cgtccgttgt tctctccgaa ttagcaaggg gcgccacaga caaaacagaa ttggattttg
    13501 tctcggcgct tgagaagaat catccggttc ttactcctat ggagaggaat tggatcgaat
    13561 cgggacatat attgcacagg atttataagg acaaggggtt cctgcctgaa aaactgaggg
    13621 acttgcattt tgacgtcctc atcgcactca cggcaagaaa tcatggggcg acggtcatta
    13681 cttcgaacat gactgacttt gagctgatca ggcgctataa agaatttcgt ctcgaaatat
    13741 ggtaagaagc tcatgccaat ccttcgataa gattatgaca accggaacga tgagcggttt
    13801 cagttctcta tttcggttat aaaacgaaga gcctcatgtt ctttctgtta tacgcgctct
    13861 tccgatttga tataatgatg cagagttgga ccgataatta tcaagaatga cattatgaga
    13921 gacatcaaga agaacagtaa gactagatcg tttagtacgg ctctcgcgtt aatatttatt
    13981 tttctggctc tccttctgac ccttttcata aaagttgtct ggccacgtct tttccatcat
    14041 gtttgatagg tcaagtgatc tggttgtttc cttcctgaat ccaaaactgg cagtgatctg
    14101 acggtgatga cgggttccaa tggaaagagt cgctactcga ttgaacgaac tcaactcaat
    14161 tgaccaacca ttatttgcag ttctctcttg acgcgtaata gaaaactgat taggtcggct
    14221 ttatcctggt ccatcatggg agatgtttgg aatcgtgtgg aacattcttc taacactgca
    14281 ttgatatgat ctctgcccga ggttgagggc caaatatgca gcaacacctc caataaactt
    14341 ctcatctctc tggtatgaac tgcccattgt tcagggaata gtgatggtcc ttctatatct
    14401 ttaataaaac catctgttct gacacttaaa cttacaaata agttagagat ccagtaaatt
    14461 cttgattgca tcgcagcaga attagcgata cggtgagtgt ctatcacata tactgtgatt
    14521 atgataagtg tgacaaataa cacaagagtt tgggccttat ctgcaatagc gttccaaaaa
    14581 attaggatgc ttagcccgcc cacgcaaact aatattccaa ggagaatact taacttttta
    14641 ttcatcttat cagaggtcct tccatactca gcgctttttc cttcttttcg tcgtctctta
    14701 aacataagtt gggattttcg gtacttggtt ggtgaaacaa acgcccttta actcatcatc
    14761 cccgcgacta ttatctaaca aagtattact tttccgcctg tatccggtct ctcctcttgc
    14821 tgatctcatc aaggagcgcg aagtaagagt cagcaaatgc cttgacccca tctttttcga
    14881 gttggtcagt cacctctttt atgctgatgc ccagggaggc gagttcctta agcaccttct
    14941 cagccttgtc catctgctcc ccaatggtga tctttactgt cccgtggtcc ttgaatgcat
    15001 tgagcgttgc ctcgggcatg gtgttgatgg tatcagggcc gataagctca tccacatatt
    15061 ttatgtcgct gtacttcggg ttcttggtgc cggtacttcc ccagagcagc ctctggacat
    15121 tcgcgccctt ttccttaaga gagaggaatc gggaagtggt gaagattctc tcatactccc
    15181 tgtaggccag ctttgcattc gccaccgccg cctttccaag gagggcggtg agcctgttct
    15241 tctcttcccc tgacgaggat gcaatgcgct gttccagaag cttgtccaca agagtgtcca
    15301 ccctgctcac aaagaagctc gccacagaga tgatctcatt gatctgctcc tttttcctga
    15361 gccgctgttc aagtcccctg atataggcct cggccacctc tttgtaccgc tcgatcgaaa
    15421 agaggagagt gacattcaca ttgacccctg aagcggtcag ctcctcaatg gcagggagcc
    15481 cctctttggt tgcagggacc ttcacaagga tgttcttttt attgatggca gagaagaggt
    15541 gcagggcctc ctttattgtc ccccgggtat catgggcaag gtcaggtgat acctctatgc
    15601 tcacgtaccc gctcaaacat tttgatgtgc cgtacaccgg gcgcaataca tccgctgcgc
    15661 gggagacatc ttccatggca aggccgagaa agagctcctt gggatcagta atccccttcc
    15721 tgagcatcat tattatggac tcatcatacg ccgcgcttcc tgaaatcgcc ttctggaata
    15781 tggtgggatt ggaggtgatt cctgtgatac cgtcctccat catgtgcata agggaccctg
    15841 tggtgatgag gtcacggtac aggttgtcaa gccagacaca ctgtcctgcc tcattcaggg
    15901 ctttcagcgg atttgcagcc atctcttctc ctccttgcat tcaataaata gtaaaaaagg
    15961 ttattgttcg ttcgggccat cgtcatccgg aacatccccc cgaaactctc gctgatacgc
    16021 gttcatggag gcatgtatta ccctatttta acggaaagtg cgggtaatct caattctcct
    16081 ttgagaaata atgttgtcaa caagtagaat tgtccggttt tagagcaaat agattgtccg
    16141 gtttctgtag aggagggtac acgtggatta cggtagccct cctgagagcg atttcaccgc
    16201 gctcatgctg ccttgtgcac atgctctggg agttcctttc ctcgcctatc atatgaggcg
    16261 agttttctgg gtccgtgaaa gagggcaaag gagccatcgg gatatcgatg gacctggacc
    16321 ctgacct
//
LOCUS       DATKOU010000043         8564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5445116, whole genome shotgun sequence.
ACCESSION   DATKOU010000043 DATKOU010000000
VERSION     DATKOU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5445116"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VMU21_01850"
     CDS             <1..435
                     /locus_tag="VMU21_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76297.1"
                     /translation="DEIFVYAFSEIVPPLVDKFRPDVVVSQLGVDSFSTDPLTHLNYS
                     QKGFCEVVKKIKEVSQRWVALGGGGYDVFNVAKAWTLAWGIMNGVDVPDEIPRDFLLQ
                     YPMEGFQSGTLRDRNYTERGETKEVMWEEVERVVGYLRGKVL"
     gene            complement(606..1367)
                     /locus_tag="VMU21_01855"
     CDS             complement(606..1367)
                     /locus_tag="VMU21_01855"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HUO76298.1"
                     /translation="MSYILDALKKSEEERKRGTVPDLLAVQDTRMQEQGKPHMWPYLL
                     LVALVLNAGIFLWWLTPWQGTKQNVTAYSTTKQNPLPEKTIISEERKQGEKHLPAQIG
                     SPVKTPIDKKVSERQGRSVQTKTAEQAQVPSASAPAVETQESRANSPSLSLQAPAAIP
                     TPSETKPSTSSKDTGGSTLYNLTELPSTIQQSLPTFSITAHIHTGDPASGMVKINGQM
                     MREGQELSPGLKLEEILADGVIFRYQNYRFRVGLK"
     gene            complement(1371..2981)
                     /locus_tag="VMU21_01860"
     CDS             complement(1371..2981)
                     /locus_tag="VMU21_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO76299.1"
                     /translation="MYKEHFGLKELPFSIAPDPRYLYMSDKHREALAHLVYGIHSDGG
                     FILLTGEVGTGKTTVCRCLLEQVPENSDIAFILNPKLSVEELLATLCDELEIQYPEGN
                     TSVKVFVDRINAYLLEAHARGRKTVLIIEEAQNLSADVLEQLRLLTNLETNECKLLQI
                     ILLGQPELKEMLSRPELRQLAQRITARYHLESLSKKEVAAYVNHRLAVAGTRSALFPS
                     ATIDRLFRLSGGVPRLINLLCDRALLGAYVQGQHHVDTRTLTKAAREVLGETTIQDGL
                     FKRMRWVMAGILLLGSAALAASFYNHKTQPVETALSRSVEPVKTAPESQKLDTLEWPD
                     SMSIYPSKGMAYQALFRQWNLPYDEEGSGDPCRQAQIHGLRCLTAQGSLNGLLSLNHP
                     AVLTLFDEKGRQFYATLSALHGQTATFVVGTEMRTVNIKEVEKKWLGDYTLLWRTPPD
                     FRSPLRPGQKGNMVLWLNKQLALLQGKNDQPGKNLVYDGDRVAQLKKFQLSEGLVPDG
                     IVGPQTIIHLNTFTASGEPLLYDKKKGQ"
     gene            complement(3158..4111)
                     /locus_tag="VMU21_01865"
     CDS             complement(3158..4111)
                     /locus_tag="VMU21_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HUO76300.1"
                     /translation="MAGESIRIGISACLLGEKVRFDGGHKLDHYLKETLGSFVEWVPV
                     CPEVECGLPIPREAMHLVGDPESPRLLTVRTGKDHTERMLRWAEKKLKELETLDLCGF
                     VFKSRSPSSGMKGVKVYSPNDMPRHRGVGIFARAVMEHFSLLPAEDEGRLSDPGLREN
                     FIERVFVFRRWKDFMLKGRTIKDLISFHTDHKLLVLSHSPRHYRTLGALVATAKEFSP
                     EHLHSEYLRILMESLRMIATVKKNTNVLQHIMGYFKNLLAPDEKKELMEVIGDYHKGL
                     IPLIIPIVLLRHYVKKYDELYLKRQHYLNPHPAELMLRNHV"
     gene            complement(4138..4566)
                     /locus_tag="VMU21_01870"
     CDS             complement(4138..4566)
                     /locus_tag="VMU21_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUO76301.1"
                     /translation="MLIEESLVIHAPLVKVWDTFTDITCWKDWNTVLKNVSPEKTEIL
                     AEGGKVRFCIYPFHFPVYFEPKIEEVIPQRRVVWSSGKFGITARHEFIFREGGNGVVV
                     TSRESFKGLSLKTLRFLFPRSRLVGLTVAFLKDLKTASEA"
     gene            complement(4573..5535)
                     /locus_tag="VMU21_01875"
     CDS             complement(4573..5535)
                     /locus_tag="VMU21_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUO76302.1"
                     /translation="MEHLEISPHDKVLLLGATGFVGSRLIPELSRRDIVLRFLVRDPS
                     KVSAHFPHLESGEVVQGDLLTGKGVGEALQGIHSAYYLVHSMGGKSIFRNMEFAQKDK
                     QAARNFISAANDTGLKRVIYLGGLGEARDTLSEHLRSRAEVAEVLSSGTPAATILRAA
                     IIIGAGGASFEMLRYLVERLPVMVCPKWIDTRIQPIALRDVLDYLLGCLFNPATAGET
                     FDVGGPEVLTYREMMQKYTEARGIAQRIIIPVPFLTPLLSAYWVDLVTPVPSGVAHPL
                     IEGLKNEVVCRDSHIDGFVHIRKTPFKEAVTLALSEEKGGPGIG"
     gene            complement(5610..6953)
                     /locus_tag="VMU21_01880"
     CDS             complement(5610..6953)
                     /locus_tag="VMU21_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HUO76303.1"
                     /translation="MSSLTSLFSPGSVALIGAAHTEIKLGGVVLKNLLKLRGKVYPVN
                     PKYDELMGLKAYHTVKEIPAPVDLAIIMRPAPEVPEILGDLEGKAKTAIVVSSGFAEV
                     GEGKLQAEVKRIGHKLGIRLLGPNCMGIFNPADRLDTFFISHERMKRPKRGNVAILSQ
                     SGAIMASLFEAIRASRIGLSKAVGYGNALDIDESDLYEYMFNDSETDVVVSYIESLGD
                     GRKFIEKAKELSGKKSLVILKSGKGESGSAAAYSHTGRLAGRYEVFRSILRQFGIMEA
                     GDFDEMMDATKALSYQRPSMGSRVCIITNGGGSGVLAADECMRQGLNVARLSEGKLTR
                     LKNMFPHFYVLNNPFDLTAQVMDEDYLTVLDEVKDNYDGFLVIALPNVMGITERLAEL
                     MKDFKGRMQKPVVFHIPTDGISRKIITRLEKTLIPVFPSPERAVKGLKALLGQGK"
     gene            complement(6950..8143)
                     /locus_tag="VMU21_01885"
     CDS             complement(6950..8143)
                     /locus_tag="VMU21_01885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HUO76304.1"
                     /translation="MPFNFVLPDLGEGTTEGEVRKWVVKEGDAIEEHQTVLEIETDKA
                     VVEVPSPKKGKVLKINKAVGDIVKVGEVLMTIAEEGEEVQEKPKIEEKPKAEERPKSV
                     SVVGVLPEEEEEVLATPAVRALAKELGIKLDSMKGSGPGGSITKEDLIKASAPEKKAE
                     DQYGPIERMPFRGLRRTIAKNMVLSQKVTAFVTGMEEADITDLWNLREKEKKPLLEKG
                     LHLTFMPFFIKAVYHALTEYPMLNASLDEEREEVIVKKYYTIGVAVDTPEGLMVPVIK
                     DAVRKTIHELAVELQELSQKARDRKIKLEEMKGSTFTITNYGHFGGIFATPIINYPDV
                     AILGTGRIADKPWVKDGKIVIRKILPLSLTFDHRVTDGVYASKFLMKVVRYLEDPALL
                     FIESA"
BASE COUNT         1963 a         2207 c         2136 g         2258 t
ORIGIN      
        1 gatgagattt ttgtgtatgc gttcagcgag attgtccccc ctctcgtgga caaattcagg
       61 cctgatgttg tggtgagcca gctcggcgtg gactcctttt ccacagaccc cctcactcac
      121 ctcaactact cccaaaaagg cttctgtgaa gtggtgaaga agattaagga agtttctcag
      181 agatgggttg ccttgggcgg cggcggctac gatgtgttta atgttgcgaa ggcatggaca
      241 cttgcctggg gaatcatgaa tggggtggac gtccctgatg agattccccg cgatttcctc
      301 ttgcaatatc caatggaagg atttcagagt ggtacattaa gggacaggaa ttacaccgag
      361 cgtggggaaa caaaagaagt aatgtgggaa gaggtagaaa gggtggtcgg ttatctccgg
      421 ggaaaggtac tttagtgtga atcctgtttt tggatttgtt cgtccttata aaacagtctc
      481 tccctggaaa cttatccact atctggttta atcttccggc actgaccaga gcgcagaaca
      541 tggcaagcag ggatatgcac aggctgaggc ataactactg aaagttgcgg tacacgctcc
      601 gtgacctact tcagtcctac acggaagcgg tagttctggt accggaagat gacgccgtca
      661 gcgagaatct cctccagctt cagtcctggt gaaagctcct ggccttccct catcatctga
      721 ccattaatct tcaccattcc cgaagcaggg tctccggtat ggatgtgggc ggtaatggag
      781 aaggtgggca ggctttgctg gatggttgag gggagttcgg tcagattata caacgtactt
      841 cctccggtat ccttgctgct ggtggagggc tttgtctccg aaggggtagg aattgctgcg
      901 ggggcctgca gtgacaagga tggagaattg gcacgagact cctgggtctc gacagcaggg
      961 gcactcgcgc ttggaacctg tgcttgttca gcagtttttg tctgaactga acggccctgg
     1021 cgctcactca ccttcttgtc aatgggtgtc ttcacaggag agccaatctg ggcaggaagg
     1081 tgcttttctc cttgtttccg ctcttctgaa atgatggtct tttcaggcag aggattttgt
     1141 ttggtggtgg aatatgcggt aacattctgc tttgttccct gccagggagt tagccaccaa
     1201 aggaatattc cagcgttcag tactaatgca accaggagca gataaggcca catatggggt
     1261 tttccttgct cctgcatccg ggtatcctga accgccagga ggtccggcac tgtgcctcgc
     1321 ttgcgctctt cttcggattt tttcagggca tcaagtatat atgacataac ttattgtcct
     1381 ttctttttat catacagtag cggttctcca ctggcggtga aagtattcaa atggattatg
     1441 gtctgaggtc ccacgatacc gtccggcacc agaccctcgg agagctgaaa ctttttcagc
     1501 tgcgcaacgc gatcgccatc atacacaaga ttcttccctg gttgatcgtt cttgccctgg
     1561 aggagcgcca gctgcttatt cagccacaac accatgtttc ctttctgacc gggccggagg
     1621 ggagaccgaa aatcaggggg cgtgcgccag agcagcgtgt aatctccaag ccactttttt
     1681 tcaacctctt tgatgttcac tgtcctcatt tcggttccca ccacaaaggt ggccgtctgc
     1741 ccgtgaaggg ctgagagtgt ggcatagaac tgccttccct tctcatcaaa gagcgtcaac
     1801 accgcggggt gatttaagct gaggaggcca ttaagactgc cttgtgcagt gaggcagcgc
     1861 aacccgtgga tctgggcctg tctgcaggga tcaccggagc cttcttcgtc ataaggaagg
     1921 ttccactgtc tgaagagtgc ctgataagcc atccctttgc tagggtaaat agacatgctg
     1981 tcgggccact ccaatgtgtc cagcttttga gattccggtg cggtttttac aggttccact
     2041 gatctggata gtgctgtctc tacaggctgg gtcttgtgat tataaaagct tgctgccagt
     2101 gctgcagaac ccaacagcag gattccggcc atcacccatc tcattctttt gaataagcca
     2161 tcctgaatgg ttgtttcccc caatacttcc cgggccgctt tggtgagtgt gcgggtatca
     2221 acatgatgtt ggccctgcac ataggcaccg agaagtgccc ggtcgcacag gagattgatc
     2281 aaacgcggaa cgccaccgct taaacggaag agcctgtcaa tagtggcgga aggaaataat
     2341 gcactgcgcg tacctgcaac tgcgagccgg tgattcacat acgccgccac ctcttttttc
     2401 gagagagatt ccaaatggta gcgggctgta atgcgctgag ccagctgacg cagctcaggt
     2461 cgtgagagca tctccttgag ttcaggctgt cccaggagta ttatctggag aagcttgcat
     2521 tcatttgttt ccagattggt caataaacgg agctgctcca gcacatcggc gctcagattc
     2581 tgggcctctt cgatgatgag aaccgtttta cggcccctgg catgggcttc gaggagatat
     2641 gcattgatcc ggtccacaaa aaccttaaca ctggtgttac cttcaggata ctgaatctcc
     2701 agctcgtcgc ataatgtggc caacagttct tcaacactca atttggggtt gagaataaac
     2761 gcgatgtcgg aattctcagg gacctgttcc agcaggcagc gacagactgt cgtcttccct
     2821 gttcccacct ccccggtaag gaggataaaa ccgccgtcac tgtgaatccc gtataccaga
     2881 tgggccagcg cctcccgatg tttatcgctc atataaaggt agcggggatc cggggcaatg
     2941 gagaagggga gttcctttag accaaagtgt tctttgtaca ttggtatcaa tacctttttc
     3001 cgtgtccttt aaaatagtaa tacaatgatc cgggaaaaaa caataacagg gtcaggctgt
     3061 cataaagaga ggtgtaatga aacagggagt gcacatggtg aatcgaacgc gcattccccg
     3121 ctgcttgcta tggcgaacca gagcgcgttt tcctctctca cacatgattc cgcagcatga
     3181 gttctgcagg gtgggggttc aggtaatgct gccttttaag atagagctca tcatatttct
     3241 tcacataatg cctgaggagc actataggaa tgataagggg tatgagacct ttgtggtaat
     3301 caccgataac ctccatgagc tctttcttct cgtcgggcgc tagcaggttc ttaaaatagc
     3361 ccataatatg ctgtaacaca ttggtgttct tttttaccgt tgcaatcatc cgcaggctct
     3421 ccataaggat gcgcaaatat tcagaatgga ggtgctcggg gctaaactct ttggctgtcg
     3481 ccaccagggc tccaagggtt ctgtaatgtc tgggactgtg ggagaggaca aggagcttgt
     3541 ggtctgtatg gaaagagatc aaatctttga tagtgcgtcc tttgagcatg aaatccttcc
     3601 accgccggaa cacaaagacc ctctcaataa agttttccct gaggccggga tcagagagcc
     3661 tgccctcgtc ctctgcaggg aggagggaga aatgctccat aactgcccta gcaaagatac
     3721 ccactcccct gtgcctgggc atgtcatttg gggaatatac cttcacgcct ttcatccctg
     3781 aactgggaga cctggacttg aagacaaagc cgcagagatc cagtgtttcc agttccttga
     3841 gttttttttc tgcccatcgc agcattcgtt ctgtgtggtc cttcccggtt cttacggtca
     3901 gaaggcgggg agattcaggg tctcccacca aatgcatcgc ctccctgggg ataggcaggc
     3961 cacattccac ttcaggacag accggtaccc attcaacgaa ggatccgagg gtctctttca
     4021 ggtagtgatc cagtttatga ccgccgtcaa aacggacttt ttcgcccagg aggcatgcac
     4081 ttattccgat ccttatgctt tctcctgcca tgatgacgta cacgtcatta agggaactta
     4141 ggcttcagaa gccgttttca gatccttcaa aaaagcgacc gtgagcccca ccagtctcga
     4201 tctcgggaag agaaagcgga gggtcttcag agaaagtccc ttgaaggatt ctctgctcgt
     4261 gaccactact ccatttcctc cctcacggaa aataaattcg tggcgtgcgg tgataccgaa
     4321 ctttccgctt gaccagacaa cacgcctctg gggaatgacc tcctcgatct taggctcaaa
     4381 atagacgggg aaatgaaaag gatagataca gaacctgacc tttccccctt ctgccaggat
     4441 ttcggttttc tcgggtgaga cattcttgag cactgtattc cagtctttcc agcaggtaat
     4501 gtcggtgaag gtatcccata cctttaccag gggagcatgg ataactaggg attcttcaat
     4561 gagcatttac catcatccaa ttccaggtcc ccctttttct tcggaaaggg ccaaggtgac
     4621 cgcctccttg aaaggggtct tcctgatgtg aacgaaccca tcaatgtggc tatctctgca
     4681 gactacttcg ttttttagtc cctcaatgag aggatgggcc acccctgagg gcacaggcgt
     4741 aacaaggtcc acccagtaag cagacaggag aggggtgagg aacgggacag ggatgatgat
     4801 cctttgagca attccgcgtg cctctgtata tttctgcatc atttcacgat aggtgagcac
     4861 ctcagggcca ccgacgtcaa aagtctctcc cgctgttgcg ggattgaaca gacagcccag
     4921 aagataatcc agtacatccc tgagggcgat gggttggatg cgggtatcga tccatttcgg
     4981 gcagaccatt acagggaggc gctccacaag gtaccggagc atctcaaagg acgcgccccc
     5041 tgcgccgatg ataatggcgg cacgcaggat agtggcagca ggcgttcctg atgagagtac
     5101 ctcagcaact tctgcgcggc tcctcagatg ttctgagagg gtgtcccgtg cttctccgag
     5161 ccctccaagg tagatgaccc ttttaagccc agtatcgttc gccgctgaga taaagttccg
     5221 cgcagcctgc ttgtcctttt gagcgaattc catgttccgg aagatgctct ttccccccat
     5281 ggaatgcaca aggtaatagg cagagtgaat cccctggagg gcttcgccca ctcctttgcc
     5341 tgtgaggagg tcgccctgaa ccacttctcc tgactcaaga tgcggaaaat gggccgaaac
     5401 ctttgacgga tcacgaacga gaaacctcag gacgatatcc ctcctgctaa gttcagggat
     5461 gagcctgctt cccacaaaac ctgtagcacc gagcaggagg actttgtcgt ggggagatat
     5521 ttccaaatgt tccatagggt cagtgtagca gatttctgta ttgaatcaaa tgaggggcct
     5581 tatttctgtc accttaaaac atgctgtatt catttgccct gtcccaaaag cgcctttagt
     5641 cccttcactg ccctctcggg tgaggggaac acagggatga gtgtcttttc aagacgggta
     5701 atgatctttc tcgatattcc atcggtaggg atgtggaaga cgacgggctt ctgcatcctc
     5761 cccttgaagt ccttcattaa ttctgccagg cgctcggtga tacccataac attggggagg
     5821 gcgatcacaa gaaatccgtc atagttatcc ttcacttcgt ccagcaccgt aaggtaatct
     5881 tcatccatga cctgtgcggt aaggtcaaag ggattattga gcacataaaa atgcgggaac
     5941 atatttttga gccttgtaag ttttccttct gagagtcttg ccacattcag cccctgcctc
     6001 atgcattcat ctgcggccaa aacacctgac cccccgccgt ttgtgatgat gcataccctg
     6061 ctgcccatgg aaggcctctg atatgacagc gcctttgttg catccatcat ttcatcaaaa
     6121 tcacctgcct ccatgattcc gaactgtctc aggattgatc ggaaaacctc atatctgccc
     6181 gcaagccttc cggtgtggga ataggcggca gcggaaccgc tttctccctt gccggatttc
     6241 aggatgacaa gagatttctt tccggacagt tcttttgcct tttcgataaa ctttcttccg
     6301 tcaccaaggg attcgatgta ggagaccact acatcggtct cgctgtcatt gaacatatat
     6361 tcgtataaat cagattcatc gatatcaagg gcattgccgt atcccactgc ctttgagaga
     6421 ccgatgcggg aagcccttat ggcctcaaaa agggatgcca tgatggcacc gctctgagat
     6481 aggatggcga catttcccct tttaggcctc ttcatccttt cgtgggaaat gaagaacgta
     6541 tcaagccggt ctgcggggtt gaagatgccc atgcaattgg ggcccaataa tcgtattccg
     6601 agtttgtgac ctatcctttt cacctctgcc tggagctttc cttcgccgac ctctgcaaaa
     6661 cctgaactca ccacaatggc ggtctttgcc ttgccttcaa ggtcaccgag aatttcggga
     6721 acctcaggag cgggcctcat aatgatggcg aggtcaacag gagcgggaat ttcctttacc
     6781 gtatgatatg cctttaaacc catgagttca tcgtacttgg ggtttaccgg gtataccttg
     6841 cccctgagct taagaaggtt tttcagcacc acccccccaa gcttgatttc ggtatgtgcc
     6901 gcaccgatga gtgcaacgga cccgggagaa aagagacttg tgagggaact catgcgctct
     6961 ctatgaacag caaagcaggg tcttcgagat accggaccac cttcatgaga aactttgatg
     7021 cgtagacccc atcggtcact ctatggtcaa atgtcagaga aaggggaaga atctttctga
     7081 ttacgatctt tccgtccttc acccagggct tatcagctat ccttcctgtt ccgagtatgg
     7141 ccacatccgg atagttgatg atgggagtcg cgaagatgcc gccaaagtgt ccatagtttg
     7201 tgatagtgaa ggtgctcccc ttcatctcct ccagctttat cttcctgtcc cgcgcctttt
     7261 ggctcagttc ctggagttcc acggcaagct catggatggt ctttctcact gcgtccttta
     7321 ttacaggaac catgagtccc tcaggagtgt cgacagcaac cccgattgta tagtatttct
     7381 ttacaattac ctcttccctc tcttcatcaa gtgaggcatt gagcatggga tattctgtca
     7441 gtgcatgata cacggcctta atgaagaaag gcatgaaggt caaatggagc cctttttcga
     7501 gcaagggttt cttttccttt tccctgagat tccaaagatc ggtgatatcc gcttcctcca
     7561 tacctgtcac aaaggcagtg accttctgtg acagcaccat attctttgca attgtccttc
     7621 tcaatcccct gaaaggcatg cgctctatgg gaccgtactg atcttcagcc tttttttctg
     7681 gggcagaagc ctttatgaga tcctcttttg tgatgctccc cccaggtcct gaccccttca
     7741 tagaatcgag cttaatccca agctccttgg ccagtgccct cactgcgggc gttgcgagaa
     7801 cttcctcctc ttcctcaggg agaacgccca ccaccgaaac agattttggc ctctcttcgg
     7861 cctttggctt ttcttcaatt tttggcttct cttggacctc ttcaccctct tccgcaatgg
     7921 tcatgaggac ttcaccgacc tttacgatat ctcccaccgc tttattgatc ttcagcacct
     7981 tgcccttttt cggcgaaggg acctccacca ccgctttgtc tgtctctatt tccaggacgg
     8041 tctgatgctc ttcaatggca tccccttcct tgacgaccca ttttctcacc tcaccctctg
     8101 ttgttccctc gcccagatca ggcaacacaa aattgaatgg catggcagaa ctccttttta
     8161 agagataaaa ttacgatatt tgagattatc cggttacatt cctaatacca acaaaagaat
     8221 atcatggcaa agaaccgtag tcaatctaat gatgtaacgt ctgccagcct ccggtgccgc
     8281 catcgatggc gtttcttaag aaaatatgcg gtgtggtgtg ttttttggtg ttgtcaacaa
     8341 gtagaattgt ccggttttag agcaaataga ttgtccggtt tctgtagagg agggtacacg
     8401 tggattacgg tagccctcct gaaagcgatt tcaccgcgct catgctgcct tgtgcacatg
     8461 ctctgggagt tcctttcctc gcctatcata tgaggcgagt tttctgggtc cgtgaaagag
     8521 ggcaaaggag ccatcgggat atcgatggac ctggaccctg acct
//
LOCUS       DATKOU010000044        11076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1594307, whole genome shotgun sequence.
ACCESSION   DATKOU010000044 DATKOU010000000
VERSION     DATKOU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11076
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594307"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VMU21_01890"
     CDS             complement(<1..313)
                     /locus_tag="VMU21_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HUO76305.1"
                     /translation="MATEIHTTSIVDKKAAIDEDVVIGPFSIIGSGATIRKGTKIISH
                     VVIEGKTEIGEECTVYPFTSIGLPPQDLKYRNEETGVRIGNKNIIREYTTIHRASVGG
                     SG"
     gene            complement(313..756)
                     /gene="fabZ"
                     /locus_tag="VMU21_01895"
     CDS             complement(313..756)
                     /gene="fabZ"
                     /locus_tag="VMU21_01895"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HUO76306.1"
                     /translation="MMDIKEIQNILPHRYPFLLVDRILEMDKGTKAVGIKNVTVNEPF
                     FMGHFPGNPIMPGVLIVEALAQLAGLLAFLSGVQGDAVYFMSIEKAKFRKPVVPGDQL
                     RLEITTLHQRANVWKFSGNAKVDEKIVAEAEFTAMVLKKDESKER"
     gene            complement(753..1772)
                     /gene="lpxD"
                     /locus_tag="VMU21_01900"
     CDS             complement(753..1772)
                     /gene="lpxD"
                     /locus_tag="VMU21_01900"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HUO76307.1"
                     /translation="MKLKELALLLHGELKGDPDTEILGVAGIHDVKEGEITFLSDPKL
                     LKECAHSKASCVIVKDVIPDMDKPQIAVKNPQYSFAQLLGHFFPKPLPAMGVSSLAFV
                     SPLAMVGKEVSIQPFAYICDNVTIGRDTVIYPGVFIGEGTVIGDGCTIYANVTIREKV
                     TVGNRVMIHSGSVIGSDGFGYVWEEGRHYKIPQVGGVIIGEDVEIGANVTIDRATTGN
                     TVIGRGTKIDNLVQIAHNVTIGEHSIIVAQVGIAGSSEIGNFVALAGQAGIPDHVKIA
                     DGCIIGAQAGVIGNLSKGIYLGSPAMPHRDWLKSTALFARLPELNKRIKELEEKVMTL
                     ERRNS"
     gene            complement(1868..2425)
                     /locus_tag="VMU21_01905"
     CDS             complement(1868..2425)
                     /locus_tag="VMU21_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HUO76308.1"
                     /translation="MNKKKGVRIIVLLFLAVLIPVTFTAVSASGEDVVKIGYVDLRVA
                     LNESEAGKKAKIELESLIKTKQSSIEERGKSLERLKGDFEKQASVLSADARKSKEEEI
                     ERLMRDYQRLVQDSQNDVKKKEGDITGSILKELRDVVQKIGQEEGYSVILENVEGMIL
                     YSRKDLDITEKVIKNYNDWKAKQKK"
     gene            complement(2509..4764)
                     /gene="bamA"
                     /locus_tag="VMU21_01910"
     CDS             complement(2509..4764)
                     /gene="bamA"
                     /locus_tag="VMU21_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HUO76309.1"
                     /translation="MVRLWAIALTYLIAAVIAASSVLAQEQLPVVNSIEVKGLKRVEE
                     GALKSKITQKVGEPLSNEKVSEDIKNIFKMGYFDDVKTEIDPMEGGIKLIYVVKEKPT
                     IIRVEFQGNKELEDDKLKEKISVTVGSIADTTLIQDNATKLQLFYEDEGYWLATVVPV
                     VNKISENEVYLTFQIDEGKKVKVRDIRIVGNKAISSGKIKGVMKTGTWKIYSFIFSSG
                     YYKKDVMNVDLDAIKDLYFNNGYIKVIVADPEILLTEDKSGMTITINISEGDQYRVSS
                     VSLTAYKAFTEAELRGRISASPGAIFSKEVVRADITSITDLYTQNGYALASVYPDIMP
                     DDATKQLSLTYKVEEGKIYRVGMIEITGNTKTKDKVIRREIRIDEGDIFNSALLKRSY
                     ERLNNLNYFETVDLVPKPKYEEQLLDVDVKVKEKSTGFLSVGGGYSSVDKFVAMVDIT
                     QANLFGTGRYLRARGELGGNISNFTLSYKDPWFMDKPVVFSVDGYLTRQQYPDYETKA
                     IGGDFGFGKSFGEYWGANIMYNLSKVTVFGVSSTASTVIKSQQGTAITSSITPSITRD
                     SRDSYIDPSSGSRNSVYFTFAGLGGDNKFVKGLADSAWFFPLGSTGTTIMVRGRIGYA
                     KGIFNETLPLYENFCVGGIYTVRGIGYCQAGPKTDQGEYIGGPKELIFNTEYVFPIVR
                     ELKLKGVVFFDAGNAYAANEAYGTLRPTAGAGIRWISPMGPLRVEWGYNLNRRPGEIS
                     NKVEFTFGSFF"
     gene            5096..6136
                     /locus_tag="VMU21_01915"
     CDS             5096..6136
                     /locus_tag="VMU21_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76310.1"
                     /translation="MGIIIDKRPVFSVLMANRNYGHFIEEALDSILQQTFPAREIEII
                     VIDDGSTDDSREKLKKYEDRVTCIYKENGGQAAAFNRGVAVSKGEFVAFLDADDYWHS
                     QKLQQVFEEFRKSDSVDFVYHYMAVINNDHHLIDWYIYPVPSSEEGTQSGKRYLNSYL
                     SGTLPWFAPTSGMTIRAECLKKAIPWPEDLRAPDVYLHYVLPFYTRELRLIKKPLGYY
                     RLHGNNISGGNLLTVRKLSHAINNIVMVEKHVEAHAERLGYDSSLIKKRIEAEVAMYK
                     ILIHGLEKRKVRALKDALLFKDFLPTDGFLYRVLRKSAMVVNILLPPAVTLWIQRKYR
                     SILYLVRWELGK"
     gene            6169..7296
                     /locus_tag="VMU21_01920"
     CDS             6169..7296
                     /locus_tag="VMU21_01920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76311.1"
                     /translation="MKKLMMVIHSLRGGGSERVAVNLLRGLDKHEFSLTLVLYEPIFD
                     YPLPGDVDVIILDIPPTWNIFKLTGNFIRKIVRLAVVIKKTHPDIVFSLLTSTNITVI
                     LAKVLSRVRCKMIASEQTHPSVNLNNEPYGGITRYLMKHTYPKADRIVAVSEGIKKDL
                     IEAFSLPQEKIEVIYNPIDIIEAEMLSHEPVYHPWFFENVPIVVAVGRLTKQKGYPHL
                     MQAFSAVRQSSPCRLVIIGKGEDEGNLARMVKALGIEGDVQFLGFQENPFKYMARSSV
                     FVLSSLYEGFGNVIVEAMALGLPVISTDCPSGPSEIIEDRKNGLLVPVKDEKALAQAM
                     VDVLSDHELRKSLSEEARRRAQSFGLDRIVKQYERRFLALM"
     gene            complement(7297..8130)
                     /locus_tag="VMU21_01925"
     CDS             complement(7297..8130)
                     /locus_tag="VMU21_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HUO76312.1"
                     /translation="MQLFTIIEIETHNLCTRKCWFCKHGQVRQDESTAFMPDDLIRKI
                     SDNLQDLGFRGRISPYSINEPFLDPRIPDIIRLFRSKCPESFISLASNGDLLSETLYQ
                     SLLVAGLDALGLSVYDDSSWKRLKAYACYSDVRIIDYRRRKFENRGGEVKRGTFSPLT
                     DRSCHRPFGMMVIRATGDVVLCCSDMYSDVVIGNVVDQRLEEIWESEKFQRYRSLLLS
                     PTHRKGLKLCQACSHDGSACLTRFPSENFEWIPEPLRGLLYRWTPPGVRAFVKRTLGK
                     E"
     gene            complement(8329..9162)
                     /locus_tag="VMU21_01930"
     CDS             complement(8329..9162)
                     /locus_tag="VMU21_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HUO76313.1"
                     /translation="MNSVNIIHIETLNLCTRHCWYCKFGQNRQDPSTLRMTDELIETI
                     ADTLHDIDYSGRISPFGINEPLLDPRMPEMISLLRSKCPRAFISLVSNGDLLTRELYE
                     TLVASGMDALGLSCHDESSWQRLKPYARYPNVRLIDMRRPKEILSNRGGEVKLRNSIP
                     QTNKPCFVPLKTLEIRSNGDVVLCCNDMYSDVVMGNIAFQPLEEIRNSKQFLNYRSKL
                     ASADRRGLKLCEVCNRTGGNCNWKFPLEDFDWIPKPLRKAVYRWTPATVKKLVKKVGN
                     L"
     gene            9512..10288
                     /gene="rlmB"
                     /locus_tag="VMU21_01935"
     CDS             9512..10288
                     /gene="rlmB"
                     /locus_tag="VMU21_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HUO76314.1"
                     /translation="MQPEGWIFGFNPVLEALKADRNIKTVYLASGRHERVSEIRKAAE
                     LKGVPVEFAGTTFFDTRFPKGHQGVAARAMKRAYVSLDDLISIPLRKGEIPLFILLDS
                     VEDPRNFGAILRTADAAGVHGVVIQSHRSVSLGPEVAKTSAGAVEYVSVSMVTNIKHA
                     IREMKERGIMIMGAESGAHSPIWDVDLTRPLAVVVGSEGKGIRRTVKENCDFIGSIPM
                     RGRINSLNVSVATGIFLFEIVRQRYGKLFGNTHLKRFPRE"
     gene            10391..10466
                     /locus_tag="VMU21_01940"
     tRNA            10391..10466
                     /locus_tag="VMU21_01940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10424..10426,aa:Thr,seq:tgt)
     gene            10525..10610
                     /locus_tag="VMU21_01945"
     tRNA            10525..10610
                     /locus_tag="VMU21_01945"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10559..10561,aa:Tyr,seq:gta)
     gene            10613..10687
                     /locus_tag="VMU21_01950"
     tRNA            10613..10687
                     /locus_tag="VMU21_01950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10645..10647,aa:Gly,seq:tcc)
     gene            10780..10855
                     /locus_tag="VMU21_01955"
     tRNA            10780..10855
                     /locus_tag="VMU21_01955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10813..10815,aa:Thr,seq:ggt)
     gene            10946..>11076
                     /locus_tag="VMU21_01960"
     CDS             10946..>11076
                     /locus_tag="VMU21_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUO76315.1"
                     /translation="MAKAKFERVKPHVNVGTIGHVDHGKTTLTAAITKVLAIKGQAT"
BASE COUNT         2777 a         2628 c         2633 g         3038 t
ORIGIN      
        1 cgccactacc accaacggag gcccggtgaa tggtcgtata ttcccgtatg atgtttttgt
       61 ttccaatcct gacccctgtc tcttcattgc gatacttcag atcctgaggg ggaagaccga
      121 tgctcgtgaa cggatatacg gtgcattctt ctccgatctc tgtcttcccc tcgatcacca
      181 cgtgggaaat tatttttgtc ccttttctga tggttgcgcc gctgccgatt atggagaaag
      241 gccctataac tacatcttca tcaatggctg cctttttgtc tacgattgag gtagtgtgta
      301 tctctgttgc cattagcgct ccttggactc gtctttttta agaaccatgg cggtaaattc
      361 cgcctctgcc acaatttttt catccacctt tgcattccct gaaaatttcc agacatttgc
      421 cctctggtga agtgtagtta tctcgagcct gagctggtca cccggcacaa cgggtttcct
      481 gaattttgcc ttctctatgc tcatgaaata cacagcatca ccctggacac cggagagaaa
      541 agcgagtagc cctgcaagct gcgccaacgc ctcaacgata agaaccccgg gcattatggg
      601 gttgccgggg aaatgtccca taaaaaacgg ttcattgacc gttacgttct ttattcccac
      661 ggcctttgtt cccttgtcca tctcgagtat cctgtcgact aaaaggaagg gataccggtg
      721 ggggagaata ttctgtatct ctttgatatc catcatgagt tcctcctctc taaggtcatt
      781 accttctcct cgagttcttt tatccttttg ttgagctccg gaagtctggc gaataatgcg
      841 gttgatttaa gccaatccct gtggggcatt gcaggagaac ctagatagat acccttggag
      901 agattcccta tcactccggc ctgtgccccg ataatacagc catcggctat cttgacatgg
      961 tccgggatgc ctgcctgtcc tgccagggca acaaaattgc cgatttcgct gcttcccgcg
     1021 attcctacct gtgccacgat aatggagtgt tcgccaatgg ttacgttatg cgcaatctga
     1081 acaaggttgt cgatcttcgt gccccttcct atgacggtat tccctgtggt cgccctgtct
     1141 atggtgacat tcgcgccaat ctccacgtct tcacctatga tgaccccgcc gacctggggt
     1201 atcttgtaat gccttccctc ctcccacaca tagccaaaac catcagaacc tatgacggaa
     1261 ccggaatgga tcatcacacg attgcccacc gttacctttt cccttatcgt cacattggca
     1321 tatatcgtgc acccgtcacc aatcacggta ccttcaccaa tgaacacacc aggataaatt
     1381 actgtatccc tcccgatcgt cacgttatca cagatatacg caaagggttg gatggagacc
     1441 tcctttccca ccatagctaa tggggagaca aaggcaaggg aactcacgcc catagcaggg
     1501 agcggtttgg ggaagaagtg ccccagcagt tgagcaaagg aatactgggg gttcttcact
     1561 gcaatctggg gtttgtccat atcggggata acatctttga caatcacaca cgatgcctta
     1621 ctgtgtgcgc attccttgag cagcttcgga tcggagagaa aggtgatttc cccctcctta
     1681 acgtcgtgga ttccggcaac acccaggatc tccgtatccg ggtccccctt cagttctcca
     1741 tgtaggagca atgcaagttc tttcagtttc atgaggagct attccgtaaa atgaatcccc
     1801 cttggtcctc tttcacaaag gcggattaag ggggattcgt accacccttt tctccttcgt
     1861 ttgagagtta tttcttctgt ttcgccttcc agtcgttgta gttctttatt accttttcgg
     1921 taatatccag atctttcctt gaatagagaa tcatgccctc cacattctcc agtatgacgg
     1981 agtatccctc ttcctgaccg atcttttgga caacatccct cagttctttc agaatagagc
     2041 cggtaatgtc accctccttt ttctttacgt cgttctggga atcctgcacc agcctctgat
     2101 aatccctcat gagcctctcg atctcctcct ccttgctctt cctcgcatcc gcggagagta
     2161 ctgacgcctg tttttcaaaa tcccctttaa gtctttctaa ggatttgcct ctctcttcta
     2221 tggaggattg ttttgtcttt atgagggatt ccagttcgat ctttgccttc ttccctgcct
     2281 cagattcatt caatgcaact ctcaggtcca cataccctat cttcaccaca tcttctccgg
     2341 aagcgctcac cgcagtaaat gtcacgggga tgagcactgc caaaaacaaa agcactatga
     2401 tcctcacacc tttttttttg ttcatttcgt cctcctcgtt attatctcaa aatctcagca
     2461 ctcttactga ctaaggatat tctatgcctg ctctttccca agaatttatc agaagaaact
     2521 gccgaaggtg aattctacct tgttagaaat ttctccgggt cttctgttca aattatatcc
     2581 ccactccacc ctgagaggcc ccatgggaga tatccaccgg attcccgccc ctgcagtggg
     2641 tcttaatgtt ccataggcct cgtttgctgc gtatgcattt ccggcgtcga agaagacaac
     2701 tcccttaagt ttcaattccc tcactatggg gaatacgtac tccgtgttaa atatcagttc
     2761 ctttgggccg cctatatact ctccctgatc agttttcgga ccggcctggc aatacccgat
     2821 tcccctcacc gtgtatatgc cgccaacaca aaagttctca tagaggggga gtgtctcgtt
     2881 gaatatccct tttgcatatc ctatgcgccc tctcaccatg atggtggtgc cggtacttcc
     2941 cagtgggaaa aaccacgcag aatcagcgag ccctttgacg aatttattat ctccgccaag
     3001 gccggcaaag gtaaaataaa cagagtttct tgaaccggat gaagggtcaa tatagctgtc
     3061 gcgggagtcc ctggttatgg agggagtaat actgcttgtg atggctgtac cctgctgaga
     3121 cttaataaca gtggaggctg tactagatac tccgaagacg gtcacttttg acaaattgta
     3181 cattatgttg gctccccaat actcaccgaa gctcttgccg aatccaaaat cacctcctat
     3241 tgcctttgtt tcgtagtcgg gatactgttg cctggtgagg tatccatcaa cagagaatac
     3301 aacgggcttg tccataaacc acgggtcttt gtatgacaat gtaaaatttg atatattgcc
     3361 gcccagctct cccctcgccc tgagatacct ccctgtcccg aaaagattgg cctgggtgat
     3421 atccaccatt gcgacgaact tatccaccga actgtaccca ccaccgacac tcagaaaccc
     3481 ggtggacttc tccttgacct tcacgtcaac atccaggagt tgttcctcgt atttcggttt
     3541 cgggaccagg tccactgttt cgaaatagtt gaggttattg agcctctcgt agctcctctt
     3601 caggagggcg ctgttgaata tatctccttc atcaatgcgt atctcccttc gtatgacctt
     3661 gtcctttgtt ttggtgttgc cggttatctc gatcattcct acccggtaga ttttcccttc
     3721 ctccacctta taggtgaggc tgagctgctt tgtggcgtca tcgggcatta tgtcaggata
     3781 gacacttgcc agggcataac cgttttgggt atagaggtct gttatggagg tgatgtctgc
     3841 cctcaccacc tctttgctga atatggcgcc cggagaggcg cttatccgcc ctctcaactc
     3901 tgcttcagtg aatgctttat aggcagtgag ggatactgaa gagaccctgt actgatctcc
     3961 ttcggaaatg ttgatggtga ttgtcatccc gctcttgtct tcggtaagca gtatctcggg
     4021 gtctgcaaca ataaccttga tatagccatt attgaagtaa aggtccttta tggcgtccaa
     4081 atcaacattc atgacatcct ttttatagta ccccgatgag aatatgaagg agtaaatctt
     4141 ccatgtgccg gttttcatga cacccttaat cttgccagac gagatagcct tgtttcccac
     4201 tattcgtatg tccctgacct tgaccttttt cccttcatcg atctggaagg taaggtagac
     4261 ctcgttttcg ctaatcttat taaccacggg aacaaccgtt gccagccaat atccctcatc
     4321 ctcataaaaa agctgcagtt tcgttgcatt atcctgtatc agggtggtgt ctgcaattga
     4381 gcccacagtg acggatatct tttccttcaa tttgtcatct tcgagttcct tgttgccctg
     4441 gaactcaacc ctgataatgg tcggcttttc cttcaccaca tagattaact ttatgccgcc
     4501 ttccatcggg tctatctcgg tcttcacatc atcaaaatat cccatcttga agatattctt
     4561 aatgtcctca gagacctttt cattggaaag cggttcaccg accttttggg taatcttgga
     4621 ctttaacgcc ccttcctcga ccctttttag gcctttcacc tcaatggaat ttacaacagg
     4681 gagttgttcc tgcgcaagga cagaagaggc cgctattacc gcagctatga gatatgtgag
     4741 agctatcgcc cataacctta ccatgctatc tgccccttcc tctcactcac ttaaaagatt
     4801 acctgttccc ttctcacctt ttcggtgact cagacaagaa ttttgggtct tagtatagtc
     4861 ctcaatggag gggtatgtaa atagaaaaat ataaggaaag gaagggaaag gggtaaagga
     4921 taagggttct ggagagagaa tatctcgtta gtaatgggtc attcgcgaaa atgcgccttt
     4981 caaggctata atagggcata tggtaaacct catggtagtt atccacagcg tactggaatg
     5041 agatagttat gcacactatt ctctagaaaa gagactcatc cacgggacct ctattgtggg
     5101 aataataatc gataaaagac ccgtattttc ggtgctcatg gcaaaccgta actatggcca
     5161 cttcattgag gaggcactgg acagtatcct gcaacagacc tttcctgcaa gggagataga
     5221 aatcatcgtc attgatgacg gctctacaga tgattcccgg gaaaaactca aaaagtatga
     5281 agacagggtt acctgcatat acaaggaaaa tggggggcaa gcggctgctt tcaatagggg
     5341 agttgcggtt tcaaagggtg aatttgtcgc ctttcttgat gccgatgact actggcattc
     5401 ccagaaactg cagcaggtat ttgaggagtt caggaaatct gactctgttg attttgtgta
     5461 ccattatatg gcagtcatta acaacgacca ccatttaatt gattggtata tctacccggt
     5521 tccgtcatcc gaagagggca cccagtcagg gaagcgttat ctgaacagct atctcagcgg
     5581 cacacttccc tggttcgctc ctacatcggg catgaccata agagcagaat gcctgaaaaa
     5641 ggccatcccc tggcctgaag acttgcgagc gccggatgtg tatcttcatt atgtcttgcc
     5701 cttttacacg agggaattac gcttaataaa gaaaccgctc ggatattaca ggttgcacgg
     5761 gaacaatata tcggggggta atctgttaac agtcaggaaa ctgagccatg caatcaacaa
     5821 tatagtgatg gtagagaaac acgtggaggc ccacgcggag agactcggat atgatagttc
     5881 ccttataaaa aagaggatag aagctgaggt ggccatgtat aagattctca ttcatggctt
     5941 agagaagagg aaagtaaggg ccttgaagga cgcactgctc tttaaggatt ttctgcccac
     6001 agatggcttt ttgtacagag ttctcaggaa gtcagccatg gttgtcaata tacttcttcc
     6061 tcctgcggta actttgtgga ttcaaagaaa atacagaagt attctgtatc tggtgcgatg
     6121 ggagcttggg aaatgaaatg acaaaagaac ggagagggta agcaataaat gaaaaagctg
     6181 atgatggtca tccacagcct gaggggagga ggttctgaaa gggtggccgt caatctgtta
     6241 agggggttgg ataaacatga gttttcctta acccttgttc tgtatgagcc aatctttgat
     6301 taccccttac cgggagatgt agacgtcatc atattagaca tccccccgac ctggaacatc
     6361 ttcaagctca caggtaattt catccgaaag attgtcaggc ttgctgttgt aataaagaag
     6421 actcaccctg atattgtatt cagtctcttg accagtacca atatcaccgt gattctggcc
     6481 aaggtattgt cccgggtgag gtgcaaaatg attgcatccg aacagaccca tccttctgtg
     6541 aatctcaaca atgaacctta cggtggaata acaagatatc ttatgaagca tacctatccg
     6601 aaagctgaca gaatcgtagc agtgtccgag ggaatcaaga aggacttgat cgaagccttc
     6661 agccttcctc aggagaagat cgaggtgatc tacaatccca tagacataat cgaggcagag
     6721 atgctctcac atgaaccggt gtatcatccc tggttctttg agaatgtgcc gatagtggtt
     6781 gcagtaggaa ggctgacgaa acagaagggt tatccgcatc taatgcaagc cttctctgca
     6841 gtgagacagt ccagcccctg caggctggtt atcatcggca aaggggagga tgaagggaac
     6901 cttgcccgga tggtgaaagc actgggtatt gagggagatg ttcagttttt gggatttcaa
     6961 gagaatcctt ttaaatatat ggcaaggtca tcggtttttg ttttatcttc cctctacgag
     7021 ggctttggca atgttattgt ggaggccatg gctttagggt taccggtcat ttctacagac
     7081 tgcccctcgg gaccttccga aataattgaa gacaggaaga atggactcct tgtgccagta
     7141 aaggacgaaa aggcattggc tcaggccatg gttgatgtat tgagtgatca tgaactgagg
     7201 aagagcttat ccgaggaagc aaggagaagg gctcaatcct ttggtctgga taggatagtc
     7261 aagcagtatg agaggcgctt tctggcgctt atgtagttat tccttgccca atgttcgctt
     7321 aacaaatgct ctcacccctg gtggtgtcca tctatagagc agacccctca ggggttctgg
     7381 aatccattca aagttctcag agggaaatct cgtaaggcaa gcggacccgt catggctgca
     7441 ggcttggcag agctttaacc ctttccggtg agtgggggaa agcaaaaggg aacggtagcg
     7501 ctgaaacttc tctgattccc aaatctcttc gagtctctga tctaccacat tgcctataac
     7561 aacatcagaa tacatatcac tgcaacacag gacgacatcc ccagttgccc gaataaccat
     7621 cattccgaag ggtcgatggc atgatctatc agtaagaggg gaaaatgtgc cacgcttgac
     7681 ctctcccccg cgattctcga atttgcgacg acggtaatcg attatcctca catcggaata
     7741 gcaggcatat gctttcaagc gtttccatga actatcatcg tagacgctga gtccaagtgc
     7801 atcaagacca gcaacgagga gggattgata aagcgtttct gaaagtaagt cgccattgct
     7861 tgcaagacta atgaaggact cagggcactt ggagcgaaac agacggataa tgtcaggtat
     7921 ccttgggtcg agaaatggct catttatgct gtacggtgaa atgcgtccgc gaaaaccaag
     7981 atcctggaga ttgtcagata tttttctaat gaggtcgtct ggcataaatg cggttgattc
     8041 atcttgtcgc acctgcccat gcttgcagaa ccaacacttg cgggtgcaaa ggttgtgggt
     8101 ttctatttcg ataatagtaa aaagctgcat gggagaaccg cgattattgc acagctcatg
     8161 gtcggcctta tactaataag ctgactgaga atttgtcaaa cagagcgggg ctcaaatctt
     8221 tgctctcaaa aaaatcgtgg aacaatacgg gagtttcttc ctggataatt cggcatcttc
     8281 tttttgaagt taatgcagct acccgacacc gctgcagaga gaatatatct ataggttccc
     8341 cactttctta acgagtttct tcacggttgc aggtgtccag cggtacaccg ccttcctcaa
     8401 gggttttgga atccagtcaa aatcctccaa ggggaatttc caattgcaat tgcctccggt
     8461 acggttgcaa acctcacaaa gttttaggcc tctcctgtca gcggaagcca gctttgaacg
     8521 gtaattgagg aactgcttgg agttccggat ttcttcaagt ggttgaaaag cgatgttccc
     8581 catgacaaca tcagaataca tatcattaca gcataaaacg acatcaccgt ttgaacggat
     8641 ctccagagtt ttgagaggga caaaacaggg cttgttggtt tgcggaattg agttgcgcaa
     8701 cttgacctcg ccaccccggt tagagagaat ctctttagga cggcgcatat cgatgagcct
     8761 cacatttgga tacctggcat agggttttag ccgttgccag ctggactcgt cgtggcagct
     8821 caatcccagg gcgtccatcc cggaagctac caaagtttca tacagctccc gtgtcagcaa
     8881 gtcaccgttg ctcaccaggc tgatgaaagc cctcggacac tttgagcgca acagagagat
     8941 catttcaggc atccttgggt ctaacagagg ttcattgatg ccgaaaggcg aaatgcgtcc
     9001 gctgtagtct atgtcatgga gggtgtcagc tattgtctcg ataagttcgt cggtcatgcg
     9061 gagagttgaa gggtcctgtc gattctgccc gaatttgcag taccagcagt gtcttgtgca
     9121 aagattcagg gtttcaatgt gaatgatatt tactgaattc atgagagaac cctgatcatt
     9181 tttggcatta aagtcattta gtatattttg tcagttgaat gggaatttgt caaacagctt
     9241 accctcaata cctcaagtgg ttcgtgtcag gctgcacgaa agcctgcggt tgtcgcgatc
     9301 ggtatggtgc gggcttctct atacatgccg gggatctttt cattgtaaat gagaaaaagt
     9361 gcatgcgacc ctttgttcgt accagattgg ttcagttgac actctttttc acctctgata
     9421 aggtatatcc tattgaagga aaaggacctg catatctcag tttgcctgtc aagtgaatga
     9481 ggtcaagttt ttaaaaagga aaacaatgac tatgcagcca gaaggatgga ttttcggctt
     9541 caaccctgtt cttgaagcct taaaagctga caggaacata aaaacggtgt atcttgcttc
     9601 aggaaggcat gaaagggtat cggagatcag aaaagcggct gaattaaagg gagttcctgt
     9661 agagtttgcc ggcaccactt tttttgatac ccggttcccc aaggggcacc agggggtggc
     9721 tgcaagggcc atgaaaaggg cctacgtaag tctggatgac cttatctcca ttcctttaag
     9781 aaaaggggaa attcctctgt ttattctttt ggattccgta gaggacccga gaaatttcgg
     9841 ggcgatcctc aggactgctg atgcggccgg tgttcacggg gttgtgatcc aatcccacag
     9901 atcggtgagc ctcggccccg aggtagcaaa aacgtcggca ggtgcggttg agtatgtttc
     9961 cgtatccatg gtcaccaaca taaaacatgc catcagggag atgaaggaaa gaggaattat
    10021 gataatgggc gcagaatcgg gagcacactc tccgatatgg gacgtggacc ttacccggcc
    10081 cctggcagtg gttgtgggat cagagggcaa ggggataaga aggacggtaa aggagaattg
    10141 tgattttata gggagcattc ccatgagggg gagaattaat tccctcaatg tctctgttgc
    10201 cacagggata ttcctttttg agattgtgcg gcagagatat ggtaaacttt ttggtaatac
    10261 gcacttgaag cgatttccac gggaataaag ggtggtacga ttttgcttga aaattttaag
    10321 ttgactattc tatagttaat cttttaaatt aattggttaa agattgaggg gtggactgtt
    10381 gcctcttgaa gccaccgtag ctcagctggc agagcagctg atttgtaatc agcgggtcgg
    10441 gggttcaatt cccttcggtg gctccatgaa ttattttatg gctgatagct tttggtttgt
    10501 tttatcacac cgatgagcct tatcggagag gtacccgagt ggccaaaggg gacgggctgt
    10561 aaacccgttg gcttatgcct acggaggttc aaatcctccc ctctccacca tagcgggagt
    10621 agctcagtgg tagagcgtca gccttccaag ctgagggtcg cgggttcgaa tcccgtttcc
    10681 cgctccatac aattagggag tgacgcgtgg agtgtagtga gaccaatcag tgaaaaccaa
    10741 ttggctcatt accgaggacg aggtgacgtg cgcggatttg cccatgtagc tcagtcggca
    10801 gagcacatcc ttggtaagga tgaggtcacc ggttcaattc cggtcatggg ctccacgaat
    10861 gccatgaatg tgtcattgag aatatacgtt tggcaatgac ggatggagaa acgaataact
    10921 cactcgcgat aaattaggag ggaaaatggc aaaggcgaaa tttgagaggg taaagcctca
    10981 cgtaaacgta gggacgattg ggcatgtaga ccatgggaag acgacattga cggcggcgat
    11041 cacgaaggta ttggcgatca aggggcaggc gacgta
//
LOCUS       DATKOU010000045        19963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2365956, whole genome shotgun sequence.
ACCESSION   DATKOU010000045 DATKOU010000000
VERSION     DATKOU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 19963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19963
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365956"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..2157)
                     /locus_tag="VMU21_01965"
     CDS             complement(178..2157)
                     /locus_tag="VMU21_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUO76316.1"
                     /translation="MKNKIIYLFMVLFLVSCGSSTTSSLAPDSPKGVSSPKVTVQSIL
                     SNMEQGRYKQGELLVRFKSGVLGASFLRTNQAVGAKTIKRYSAVPNLEHVKLPEGVSV
                     RDAITKYMSDPNVEYAEPNFIRRAANVPNDRLFDPQQWALQNKGTFADGVVGADIKAP
                     GAWDITVGSDGIIIAVLDTGIDLNHPDLAGNIWHNPGETSCVDLVDNESNGFIDDCTG
                     WDFTTCAEFAVDGSCVIPKEQNNNPADDNGHGSHVSGIIGAVGNTDGTGIAGVMWTVK
                     LLPLKVLNSFGEGSVSDVIDAIEYVVTNVNKGVNIKAINASFGSTGFSSAEKDAIDNL
                     RTAVNDAGSAGVLFVASAGNAPSINDTGTNDDITPVFPASYSNPTFGGLSNIISVAAT
                     DQQDRLASFSNYGMNSVQVAAPGVYILSSVPHGFAFSLCTGSEFVGYDFCSGTSMAAP
                     LVTGLVGLLYSFYDGTHDTRFSPAQVRGTILRYVDTVFNDPDSPNLQSLNGKIQSGGR
                     INAFKAVSSLLIPTDLVATAQSSTQISLTWTDRATGEDGYYVERMPVGGSFVRIATLA
                     PNATTFTDSGLSASTTYTYQVKAFNSIAESLPSAPPQSATTLAPDDPPSDPPSSGGGG
                     GGCSIGARQNAPTAMADLGVLLMPLLLFAILRRRR"
     gene            complement(2457..3188)
                     /locus_tag="VMU21_01970"
     CDS             complement(2457..3188)
                     /locus_tag="VMU21_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76317.1"
                     /translation="MKRLNIYRMTVAVSLLIMCLTVSCASTPREDTLQKAKAHYQMGV
                     SYLNDNNVQPAFVEFQKALELNPNDKETLNAIGVIYLLKLEDYARAIEFLQKALKVDK
                     NYSEAWNNLGVTYERMGKYNEAIGAYKTALMNTFYRNPEKAFNNLGRAYYRIKRYDDA
                     IDAYKEAAKRASDFALPYYGLALCYNATGKYGNAAAALRKALEIDPAYRGDKERAIRD
                     LKERKVIPRAEEEKDMDDLLEIMNY"
     gene            complement(3239..6424)
                     /gene="mfd"
                     /locus_tag="VMU21_01975"
     CDS             complement(3239..6424)
                     /gene="mfd"
                     /locus_tag="VMU21_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HUO76318.1"
                     /translation="MISVSNIRKFFSIPVGTSHIYNLAGSSAALLLSFSKEPFLAVEQ
                     TEESALRLYEDIGFFLRVMRPPQETLRFLPEPNGIESSGKRAEVLYGIKEGDSLVTSV
                     KGMDSPVWRPGDLKREAMVLRPGQEAEREIVARGLRSLGYKRVSIVMEKGEFSTKGWL
                     LDIFPSTMEYPVRIEFFGDEIEQIKSFDIDSQRSIGTIGQATIMPVTEPSDDTKIESL
                     VNGMVFLVDLLPSHSSSLLLKGTSGQDTGAEHGEVEEHAYNTERAVSLSRFDVKGEGY
                     DAGMRTIRGLGIYPEERKSLGDLAGVVTTLRKDHRIVIVSPSEAQAERVKEILFEGGV
                     VASVIGAEDLVNYGGSVAITQGKLSSGCFIPGLLILTEREIFGERPPYRPLKQSKVSK
                     LLASMDDISPGDFVVHPDHGIGRFLSVQKERTGDFENDLIVLEYAGNDRLYIPLYNID
                     RIKKYNAEEGATPLLDKLGGKTWQRRKERVKKAVKEMAEKLLKLYAERAVAKSFSFSA
                     DTELHREFYGFFPYEETPDQVKAAEEIGQDMESERPMDRLLCGDVGYGKTEVAMRAAF
                     KAVYDGKQVAVLVPTTILCEQHYHTFKGRFSAFPVKIDYISRFKSKRERDVTMRAVAR
                     GEIDIIVGTHSLLRKETSFCNLGLLIIDEEHRFGVGQKERIKELKQGVDILSMTATPI
                     PRTLHMAISGIRAMSVIETPPEERLAVRSMVSIFDAGLVREAMERELQRDGQVLFVHN
                     TIFDIEKIAALLKRLMPDARFGIAHGQMAEGRLEEVMLKFIKREIDVLISTTIIGSGI
                     DIPTANTILIDRADRIGLSDLYQLRGRVGRSNVRAYAYFLIPGKDLVSEDAKKRLQAI
                     SEMSYLGAGFRLAMKDLEMRGAGNLLGPQQSGHIHAVGFDLYVEMLEKAVAELKGIEM
                     REEREPYISLKVTARIPEEYIEDMTVRMSVYRRIANAYSPEDLSDIEAEMTDRFGAVP
                     DEVGRLLDFMRLKGMARRLSILKISETDRKIQVVFSKDTSVATEKVLALQQTFHGIRF
                     HRDGFELSLKGISTNEAHREVSRAMSSLLPA"
     gene            complement(6646..7101)
                     /locus_tag="VMU21_01980"
     CDS             complement(6646..7101)
                     /locus_tag="VMU21_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HUO76319.1"
                     /translation="MGTILSRNELKEEREKLRAAGKRLVFTNGCFDILHVGHVRYLRS
                     ARELGDVLMVALNTDQSISRIKPGRPVIPEGQRAELVASLEMVDYVTLFDEDTPYELI
                     KLLGPDVLVKGGDWKREEIVGSDIVPETYSLPYIKGVSTTEIMEKIRKL"
     gene            complement(7212..7658)
                     /locus_tag="VMU21_01985"
     CDS             complement(7212..7658)
                     /locus_tag="VMU21_01985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUO76320.1"
                     /translation="MRPATIRNQVSSGGVIFKKAGENDVEIALVAVKEGKIWSLPKGI
                     VEKGEETSETAVREVREETGLSGKVVEKIGHITYWYYIREDNAKCRKTVHFYLLEYVS
                     GSTADHNWEVERAEWFPIHDALKNLSYKGDKEIVKKAREMLFAKSH"
     gene            complement(7658..9001)
                     /gene="rseP"
                     /locus_tag="VMU21_01990"
     CDS             complement(7658..9001)
                     /gene="rseP"
                     /locus_tag="VMU21_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HUO76321.1"
                     /translation="MTFFSAIILLGILIFVHELGHFLFAKLVNVKVLKFSLGFGPRIL
                     GRKYGETEYLLSAVPLGGYVKMLGEEQGDEITDEEKARAFNFQSVWKRIIIVLSGPVF
                     NILFAAFVFALIAVSGVPVPYPDIGKVSEGSPAAKAGLMTGDRVLAIDGASVKGWDEI
                     ESAISKNQGKTILFTIKRGERVVDVQVGPEKKSEKDVFGEQREVWDIGISPLLYPDVG
                     EVTKGSPAEKAGIQKGDRILEIEGSALKTWQDMTSLIYDNPGKPMRFRVKRGDGFLDL
                     TITPVKSTFTGPGGEKKEIGLIGVRPFGNDFIKRFGPAEAMGFGIMKTWDISALTVVS
                     LVKLIQRIIPAETIGGPILIFQMAGQQAAHGLLSFFTFMAVISINLGILNFLPIPILD
                     GGHILFLGIEAVRRKPLSEKVMMVAQKVGLAIIITLMVFAFYNDIMRLITGKMIP"
     gene            complement(9068..10225)
                     /locus_tag="VMU21_01995"
     CDS             complement(9068..10225)
                     /locus_tag="VMU21_01995"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HUO76322.1"
                     /translation="MKRVVILGSTGSIGRMALEVISRHSDDFCVVGLAAGKNVDLLGR
                     QVKAFRPQIVAVAKEEGARELRRTVGRKTEVCFGEEGMKAVAACRQADFVLSAMVGFS
                     GLVPTVHAVRAGKVIGLANKESLVIAGSIVMKEAKAHRATILPVDSEHSAVFQCLEGQ
                     DRRYVKRIILTASGGPFMGRKKAELREVSPEEALQHPNWSMGKKITIDSATMMNKGLE
                     VIEASHLFCLPHEMIDVVVHPQSIVHSMVEFRDGALLAQISVPDMRGPIAYALSYPRR
                     LASTVARLELASIQQLTFHKPDTKNFPCLQLAYDALREGGTMPAVLNAVNEVAVNAFL
                     EKRINFGRIPVIIKRVMNSHTRGKAVELGAVIEVDKWAREKAEEHIRRART"
     gene            complement(10227..11012)
                     /locus_tag="VMU21_02000"
     CDS             complement(10227..11012)
                     /locus_tag="VMU21_02000"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HUO76323.1"
                     /translation="MHAKRLVVAVLLLPLFYFSVMGLPEWCFFALLLIACTLAQWELY
                     SMYRFTGLMKNSGILFGVLMLTAAYISGDPFPPVSMLMVMVIFAIRLFGLRNPSSCLR
                     DISFTLLSLFYIPGLLAFQVLLRHSGPQWIIFLYGCVWISDSAAYYVGKSIGKRKLYL
                     EISPNKTIAGAVGSLIGGSLSGWMLNMVLVHSMGTGESFVVGGIIGTVTIIGDLVESM
                     FKRDAGVKDSSGIVPGHGGILDKIDGVLFAGPVLYLVSEIFGG"
     gene            complement(11014..11727)
                     /locus_tag="VMU21_02005"
     CDS             complement(11014..11727)
                     /locus_tag="VMU21_02005"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HUO76324.1"
                     /translation="MLSGRIPEHVAIIMDGNGRWAEMRGLPRIEGHKRGAERAREVIS
                     AAMEVGVNTLTLYAFSLENWQRPDDEVSTLMKLLEFYLSKEFSELIRRGIVFRTIGEV
                     WRLPASVQALLKNAEEKSSHNKGMTLVLALSYSGRNEIIRAVKKIISLGVKEEDVTEE
                     YFGSLLDTAGIPAPDLIVRSSGEKRISNFLLWQAAYAEFYFTDTLWPDFTKEEFFLAL
                     HNYQMRERRFGTVQARAES"
     gene            complement(11747..13531)
                     /locus_tag="VMU21_02010"
     CDS             complement(11747..13531)
                     /locus_tag="VMU21_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HUO76325.1"
                     /translation="MRRQDAEHSGKKRGGKRGTMHSVYALCSVIGLLFLLSGAAHAQD
                     PLRQLKDETLSYFTPLKGKVVAVMGKVITSDLGSSAGVKKGMRFTILKEGTPFLHPIT
                     KEPLGRTETPEGKAVVREVKEKSSAMEIIKGDASLGDIVRISEMKIRVLFYQDKSANW
                     NLADSYYDLLKESGRFEIVDASLNSADNDALLAEARKVSAAVVFAVTGREQDNEMFLK
                     QRILWVEDSSGPAENEVKVEAAYAKALGGSRTMVAPIASASDTLLSFDLPFGAKLISV
                     GDLKGDGTNEVILSTGRDLRVFSLGSGLQDLYELKVAASEDFLWIDTADVDGDGKDEI
                     IVTSLRGRSVDTTSDSLVQAVKDEGKVVSYIYALKGSELSLLWKGNVFLRSLPQTGLI
                     AQKFDSAEGFGGPVFRMVYSSGEVRAGEEIKLPKGVNIYDFVYVDGSGAAKNVLAYDD
                     SGYLNLYNEMGLRIWRSNDNYGGMLTTFKKAAPTVMVDRGVWSIKDRLFVRNRDSFAV
                     KRIPLASVAKGLGYKSSQIKTLLWTGFSMEENTLVEGISGAIQDYAFAGDKLIVLSKP
                     LFGIKFKNIMKGENPLGSMLYIYSLKGM"
     gene            13973..14824
                     /locus_tag="VMU21_02015"
     CDS             13973..14824
                     /locus_tag="VMU21_02015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HUO76326.1"
                     /translation="MRISLIIPTFNASSHIEDLLSMLCAQDTKPWEIIIVDSSSEDTT
                     VEIARRFGARIMVIPRHAFNHGKTRNMAAMEAKGDILVFMTQDAFPDQNTLLSTLTAP
                     LHIHDIAASYGRHLPGTNASPLEIFARRFNYPDSPMTKSLNDIQKYGIKTFFFSNACS
                     AIKRDLFLSAGMFREGVRANEDMLMAARLILSGYRVAYVPEATVIHSHNYSLLQQFRR
                     YFNIGSSLKNNAWVLEYARPEGEGLRFIRGQLGFVVKHHGYLWIPYIFLESLAKYTGY
                     RAGLIAG"
     gene            14821..16266
                     /gene="wbaP"
                     /locus_tag="VMU21_02020"
     CDS             14821..16266
                     /gene="wbaP"
                     /locus_tag="VMU21_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HUO76327.1"
                     /translation="MIQIVKLFRRLIKVACLILIDLISFYLSLFVAWTVRTEILSSLM
                     SNLPGFPFPYLYYLSLWWMPLIFMFFLLYEGLYEKNLPFWDEARITLKAVSLASLTIM
                     TIVTLGKMQDKVSRIILLGLWTASLFIFPLLRLWGKKCLYHIGVWRERVLILGAGNAG
                     RLTMEGMERERHMGYEVMGFLDDDEQKRGKVFKGKKVFGRIKHFPKFIKELNISTVII
                     AMPSLSPERLSNLTANVQNHSPDTMVIPDLKGIALLNTELLHLFYEELFLMNIRNNLK
                     SAPNRLIKRMFDVLLSTAMMPLLLPLIGIIGAVIRWETPGPAIYSHDRIGKNGKTFRC
                     YKFRTMQRDAEEKLREILEKDEEIRSEWERTWKLKEDPRITRIGRFLRKTSLDELPQI
                     FNVMRGEMSLVGPRPYLLREQETLESQLHVICSTKPGITGLWQVSGRSNTGYEHRLKL
                     DAWYVMNWSLWFDIAILFKTIRVVTKREGAY"
     gene            16338..17861
                     /gene="gspE"
                     /locus_tag="VMU21_02025"
     CDS             16338..17861
                     /gene="gspE"
                     /locus_tag="VMU21_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HUO76328.1"
                     /translation="MIQDKTYLALEDLPKVPFVVEGISSRFIRECMVIPLELKNNILK
                     VIMANPEDRDTIDALKVATSSNVLVYTADPKVVDEYISKFYGQESQNIDKIMEDMGDK
                     TLDFTREEDEDTGHLKDLAAEAPIIKLVNLLITRAVEGRASDIHIEPFEDEVKVRYRI
                     DGVLHDIESVPKRLQAAIVSRIKIMAKLNIAERRLPQDGRIRLKVGEGEIDFRVSTIP
                     VLYGESLVMRILRKEGIVIDLDLLGFPPKTLADLSQLITKPNGIILVTGPTGSGKTTT
                     LYGALDKINSPDKKIITVEDPVEYQLKGVNQIQVKPQIGLNFATTLRHIVRQDPDVIM
                     IGEVRDLETAEIAIQSALTGHLVFSTLHTNDAPSAITRLIDMGVENFLLSSTVRGILA
                     QRLVRVVCPECKERDSSSADREALGALGIGSDVPLFKGTGCEKCAFTGFYGRTGIYEL
                     LIVNDEMRKLVLKGADANQLREVAMRLGMKTLLQSGTEKIRAGMTTLSEVLRVTQEA"
     gene            17862..19058
                     /locus_tag="VMU21_02030"
     CDS             17862..19058
                     /locus_tag="VMU21_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUO76329.1"
                     /translation="MATFSYRATNMEGAIVEGVIEAADEKAAIESLRNVGVIPLKVAA
                     PGEGMGKAFLSLKRSKGDILTFTTELSALLSAGLPLDRSLNIISEISESKEMKGIVQS
                     ILKSIREGHSFSEALQKHPRTFPKIYVNMIRAGEAGGVLDVVLDKLNEFLESSKELKE
                     HIFSAMIYPSILIVTGGISVIVLLTYVLPKFSVIFSEMGASLPLPTAIVLGFSSFLQA
                     YWWVMLLAVVSGWLIFKNYIKSPSGRYQWDSFKLKLMGEVIRKIETARFSRTLGTLLR
                     SGVPLLQALNNAKDVITNQVIASAIDKVSKGAKEGKGISVPLSDANVFPPLALSMIKV
                     GEETGQLDEMLMKVASTYEKSLRVAIKRFVSFIEPAMILGMGLVIGFIVVSMLMAIFS
                     ITELPF"
     gene            19076..19555
                     /locus_tag="VMU21_02035"
     CDS             19076..19555
                     /locus_tag="VMU21_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUO76330.1"
                     /translation="MPPCTPSLTSLAPGKEADAGAGFTLLELLIVLALVTLVVGISTV
                     FLVNTLPASKLNATARDISSTIRYARTLAQTNNEQQVMTIDLDSNRYGIEGRGDRNIP
                     PGMHIKVMDPFSGEIRNGTYQMRFHAFGTGGATVVVWNDKRSVSIQVDPITGPVITK"
     gene            19561..>19963
                     /locus_tag="VMU21_02040"
     CDS             19561..>19963
                     /locus_tag="VMU21_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HUO76331.1"
                     /translation="MVRSKGRVSGIILFAPGVRRFRTLSSSAKGFTLLEVLVAVALLG
                     IAITVVLQLFSADLKAIAASEEYVSATARAEAKMREVQDNDTLSEGTTSEMTNDGYRL
                     DVSVASTLQERTDTLQVMLMDILVTVHWTKGT"
BASE COUNT         4858 a         5467 c         4622 g         5016 t
ORIGIN      
        1 acctgagatg tatcgtctcc ctgaacagga ggggaaccgc gaaaaggtag aggggaatca
       61 gatatttaag aagagacagg gaaaaggcat gatacgcaca caccaggacg aacagcaaca
      121 gatatgtgga taatgccttt ccccttggga tatccagttt aacccttata tctcaaatta
      181 tcttctgcgc ctcaaaatcg caaagagcag caacggcata aggagcaccc caaggtcagc
      241 cattgcagtg ggcgcattct gccgtgcgcc gatggaacat ccgccgcccc caccgccaga
      301 ggagggtgga tcagatggtg gatcgtccgg agcgagagtt gtagcagatt gtggcggagc
      361 agagggaaga gactcagcaa tgctattaaa tgcttttacc tgataggtgt aggtagtgga
      421 agcagagaga cctgaatcgg taaaggtcgt tgcattcggt gccagtgtcg ctatcctgac
      481 aaaactgccg ccaactggca tcctctcaac ataatatcca tcctctcctg ttgctctgtc
      541 tgtccaggtg aggctgatct gggttgagga ttgagcagtg gcaacaaggt ctgtcggtat
      601 cagcaacgac gacaccgcct taaacgcatt gatccttcct ccactctgga ttttgccatt
      661 caaactttgg aggttaggac tatccgggtc attaaataca gtatcaacat acctgagaat
      721 ggtaccgcga acctgagctg gagaaaacct cgtatcgtgg gtaccgtcat aaaaactgta
      781 gagcaatccc acaagaccgg taactagggg agcagccatc gaagtgccgc tgcagaagtc
      841 ataacctacg aattcagacc ccgtgcacag agaaaaagca aatccatgcg gaactgaact
      901 tagaatatat actcccggcg cggcaacttg aacggaattc atcccgtaat tgctgaatga
      961 tgccaatctg tcctgctggt cggtggcagc aaccgagatt atattggaaa ggccgccgaa
     1021 agtcggattg ctgtagcttg ccggaaacac cggtgtgata tcgtcattgg tacccgtgtc
     1081 gtttattgaa ggcgcatttc cggcagaagc cacaaacaaa acacctgcac tacctgcgtc
     1141 gttaacagca gttcttaaat tgtcgatagc atccttttca gcactcgaga atccggtact
     1201 tccaaaactt gcgtttatcg cctttatatt gaccccttta ttgacattgg tgactacata
     1261 ttcaattgcg tcaataacat cggatactga accttccccg aaggaattaa ggactttaag
     1321 cggcagcaat ttgaccgtcc acatcacacc cgcgatgcca gtgccatcgg tgttgccgac
     1381 cgccccgata atccccgaaa catgtgaacc atggccattg tcatccgcgg ggttattgtt
     1441 ctgttctttt ggaataacgc agcttccgtc tacggcgaat tcagcacagg tggtaaaatc
     1501 ccagccggtg cagtcgtcaa tgaatccgtt agactcgtta tcaacaaggt caacgcagct
     1561 tgtctcaccc gggttatgcc aaatgttgcc ggcgagatcg ggatggttga gatcgattcc
     1621 tgtgtcgagg acagcaatga taataccgtc actcccgacg gttatgtccc acgcacccgg
     1681 cgcttttatg tctgcgccga caacaccatc agcaaaagta cccttattct ggagcgccca
     1741 ttgctgggga tcaaaaaggc gatcattggg aacattggca gcacgccgaa tgaagttcgg
     1801 ctcggcatat tccacattgg ggtcggacat atatttggtt attgcatccc tgacagaaac
     1861 accttcagga agttttacgt gttcgagatt ggggacagca gagtatctct tgatcgtttt
     1921 tgcgcctacg gcctgatttg ttctcagaaa ggaagcaccc aaaacacctg acttgaatct
     1981 gacgaggagt tccccctgct tgtatctgcc ctgttccatg ttggataaaa ttgactgaac
     2041 cgtaaccttt ggagagctta ccccctttgg actatcaggg gcaaggcttg aggtagtgct
     2101 gctcccgcag gataccaaaa aaagaaccat gaaaagatag ataatcttat tcttcatctc
     2161 atcctctcct tattgaaaga gttgttttta tctattccag cttaaattga atcatattaa
     2221 ttctttattg tcaagtgttt ttaggttttt cttatagttt ttgttaatat tttggagtgc
     2281 ggatttccta ggggcaacga ttgtactatt gagtacttca tactccagaa ttcttcctgc
     2341 ctggcacact gacgtgattt atttaagatg agaagggagt atagatggaa aatctcacaa
     2401 attgagggtt aagattaagg ttcaaggttt atcttggttt ctgcctcagc ccgggcctaa
     2461 taattcatta tctccaggag gtcatccatg tccttctctt cttctgccct tgggatgact
     2521 ttcctctcct tcagatccct gatcgccctt tccttgtctc ccctgtatgc agggtctatc
     2581 tcgagggctt tcctcagggc tgcggcagca ttcccgtatt tccccgtggc attgtaacag
     2641 agcgcaagcc cgtaatacgg gagggcaaaa tctgaagccc ttttggccgc ctctttgtaa
     2701 gcgtcaatgg cgtcgtcgta tcttttgatt ctgtagtaag ccctgcccag attattaaag
     2761 gctttttcag ggttccggta aaaagtattc atgagcgctg ttttgtaagc tcctattgcc
     2821 tcattgtatt ttcccattcg ttcataggtg acccccagat tattccatgc ctctgaatag
     2881 ttcttgtcca ccttgagcgc cttctgaaga aactcaattg ccctggcgta gtcttcaagt
     2941 ttcaggaggt agatgacccc gattgcgttg agggtctcct tgtcattggg attcagttcc
     3001 agcgctttct gaaactccac aaatgccggc tggacattat tatcattgag atacgacact
     3061 cccatctgat aatgggcctt cgccttctgg agcgtatcct ctctgggtgt gctcgcacag
     3121 gaaacagtga gacacatgat cagcaaggaa actgccactg tcatgcgata gatgttgagc
     3181 cttttcatag cgctcagtaa aattatcaga aatctccgga acaagtaaag gagaatcttc
     3241 acgcaggaag cagactcgac attgcccggg agacctccct gtgtgcctca ttcgtagaga
     3301 ttcccttgag actgagttca aaaccgtccc tgtggaacct gatgccatga aatgtctgct
     3361 ggagggccaa taccttttcc gttgccactg aggtatcttt tgagaagacc acctgtatct
     3421 tcctgtcagt ttcggagatc ttcagtatgg agagtcttct tgccataccc ttcagcctca
     3481 tgaagtcgag aagtctcccc acctcatccg gcactgcgcc gaaacgatcc gtcatttctg
     3541 cctcgatatc cgacagatct tcagggctat atgcatttgc aatcctccgg tacacactca
     3601 tcctcacggt catatcttca atgtactcct cagggatgcg tgcagtaacc ttcagtgaaa
     3661 tgtacggttc cctctcttcc ctcatctcga tgcccttcag ctccgcaacc gctttttcga
     3721 gcatctccac atacaggtca aaccccacgg catggatatg tcctgactgc tggggaccga
     3781 ggaggtttcc tgcgcccctc atttcgagat ccttcatcgc aagcctgaat cctgcgccaa
     3841 ggtaactcat ctcactgatt gcctggagcc tcttttttgc atcctcgctc acaaggtctt
     3901 tcccggggat gaggaaatat gcgtacgccc tcacattgct tcttcccacc cgtcccctca
     3961 gttgatagag atcggagagt cctatcctgt cggccctgtc tatgagtatg gtattcgcgg
     4021 tggggatatc gattcccgaa cctatgattg tggtggagat gaggacatcg atctctctct
     4081 taatgaactt cagcatgacc tcctcaagcc tcccctccgc catctgccca tgggcaatgc
     4141 caaatctcgc atccggcata agcctcttta gaagggccgc aatcttttca atatcaaaaa
     4201 tcgtgttatg cacgaagaga acctggccat ccctttgaag ctccctttcc attgcctccc
     4261 ttaccagacc tgcgtcaaat atagatacca tgctcctcac cgcaagcctc tcctcggggg
     4321 gggtctcgat cacgctcatt gccctgattc ctgagattgc catgtgaagc gtcctgggta
     4381 tcggggtcgc ggtcatactc aggatgtcta ccccttgttt cagttccttg atccgctcct
     4441 tctgccccac accaaacctg tgctcttcat caatgatgag aaggccgaga ttgcagaatg
     4501 aggtttcctt cctcaggaga ctgtgggttc ccacaatgat gtcgatctct cccctcgcta
     4561 cggcacgcat ggtgacatca cgttcccttt tcgatttgaa tctgcttata tagtctatct
     4621 tcacagggaa tgcggaaaac ctccccttga acgtatggta gtgctgttca cagagaatgg
     4681 tggtgggaac aagaaccgca acctgttttc cgtcatagac cgctttgaat gccgccctca
     4741 ttgccacttc ggttttcccg tatcccacat ctccgcataa gagcctgtcc atgggtctct
     4801 ctgactccat atcctgcccg atctcttctg ctgccttcac ctggtcaggc gtctcctcat
     4861 aggggaaaaa accataaaac tccctgtgga gctctgtatc agcgctgaaa gaaaaactct
     4921 tcgccaccgc cctttccgcg taaagcttaa ggagtttttc ggccatttcc ttgaccgcct
     4981 tctttaccct ctcttttctc ctctgccagg ttttcccccc gagtttgtca aggaggggag
     5041 tagccccctc ctcagcattg tattttttta tcctgtcgat gttataaaga ggtatataga
     5101 gccggtcatt ccctgcatat tcaaggacga tgagatcatt ttcgaaatcg cccgtccttt
     5161 ccttctggac agagaggaat ctccctatgc cgtgatcagg gtgaaccacg aaatcaccgg
     5221 gggagatgtc atccattgag gcgagtagtt ttgacacctt ggactgcttc agcggcctgt
     5281 agggcggtct ttcgccaaag atctccctct cggtaaggat aagaaggccg ggaataaaac
     5341 accctgatga cagcttcccc tgggtgattg cgacagagcc cccatagttc acaaggtctt
     5401 ctgcgccgat caccgatgcc accacccctc cctcaaagag aatctccttc accctctcgg
     5461 cctgcgcctc cgaaggggat acgatgacta tacgatggtc cttcctgagc gttgtgacca
     5521 cccccgcgag gtctccgagg gacttcctct cttccggata gataccaagt ccccggatag
     5581 tgcgcatccc tgcatcgtac ccctcaccct ttacatcgaa ccttgagagg gaaactgctc
     5641 gctccgtgtt ataggcatgt tcttcaacct ccccgtgttc cgctcctgta tcctgcccgc
     5701 tggttccctt aagaagaagg gaggaggagt gcgagggaag taaatccaca agaaatacca
     5761 tgccattgac aagggattct atctttgtat catcggaagg ttctgtgacg ggcataatgg
     5821 tagcttgccc gatggtgcct atggatctct gggaatcgat atcaaaggat tttatctgtt
     5881 caatctcatc tccaaagaat tctattctga cagggtattc catagtggaa ggaaagatat
     5941 ccagaagcca ccctttggtg ctgaactcac ccttctccat tacgatggag accctcttat
     6001 atccgaggct cctcagccct cttgccacga tctccctttc cgcttcctgg ccgggtctga
     6061 ggaccatggc ttccctcttc aaatcccctg gcctccatac cggtgaatcc atccccttta
     6121 ctgaagtgac aagcgagtct ccctccttaa ttccatacag gacctcggcc ctcttgcccg
     6181 aggactctat cccgttgggt tcaggaagaa agcggagagt ctcctgaggg ggtcgcatca
     6241 ctctcaggaa aaaccctatg tcttcgtaaa gcctcaaggc cgattcttcg gtctgttcaa
     6301 ccgcaagaaa tggttctttg ctgaagctca agagaagggc tgcagaagag cctgcaaggt
     6361 tgtaaatgtg agatgtacca acagggatgc tgaaaaactt ccttatattc gaaactgaaa
     6421 tcattttatc tcttatgtgc cagagccttt tgaaaaaagt tacttggggt ttcttgctca
     6481 ctaccacaca acttatactg acatgcgcag acaggcaagc cgtaaacttg tcgagatggg
     6541 ctatcgtaat tttttttgaa aaggctttgg catattatca aaatcaacgc tgtggaccta
     6601 acccggttat attggcatgc acacgaccgg ggcaagaaat ctgactcaga gcttccttat
     6661 cttttccatt atctccgtgg ttgatacgcc ctttatgtaa ggaaggctat acgtttccgg
     6721 aacgatgtcc gaacccacaa tctcttccct cttccagtct ccacccttca ccagcacatc
     6781 aggccccagg agtttgatca attcataagg ggtgtcctca tcaaacaggg taacataatc
     6841 aaccatctca aggctggcaa ccagttctgc cctctgcccc tcaggaatta caggcctccc
     6901 gggttttatc cgcgatatgg actggtctgt gttgagtgcc accatgagga catctcccag
     6961 ttcccttgcg ctcctcagat atctcacatg tcccacgtgg agaatatcaa agcagccgtt
     7021 cgtgaagacc agcctcttcc ccgctgccct cagcttctcc ctctcttctt ttaactcgtt
     7081 ccggctaagt attgttccca cacacaccag tctcctgcag agggtaaacg ctcaaaacag
     7141 tcgtaaccct catgtcaggt tctgagacca gccacggaac cagctgactc atgaggaccg
     7201 tctttcgttg attaatgact ttttgcaaaa agcatctccc gggccttctt cactatctcc
     7261 ttgtccccct tgtaactgag gtttttcagg gcatcatgaa tgggaaacca ctcagccctt
     7321 tccacttccc agttatggtc tgcggtgctg ccgctgacgt attcaagaag ataaaaatgc
     7381 acggtctttc tgcacttcgc attgtcttcc cggatatagt accaatacgt aatatgccct
     7441 atcttctcta ccaccttgcc ggaaagtcct gtctcttctc tcacctccct caccgcggtt
     7501 tcggatgttt cttctccctt ttccacgatc ccctttggca gcgaccagat cttcccttcc
     7561 ttcaccgcca caagggctat ctccacatcg ttttcccccg cttttttgaa tatcacgccc
     7621 cctgaggaaa cctggttcct tatggtagcc ggtctcatta cggaatcatc ttcccggtaa
     7681 tgagtctcat gatgtcgttg taaaaggcaa acaccatgag ggtgatgatt attgcgagcc
     7741 ctaccttctg tgcaaccatc attaccttct cgctcaaggg ctttctcctc accgcctcga
     7801 ttccgaggaa gaggatgtgc cccccgtcaa ggatcggaat gggcaggaaa ttcagtatgc
     7861 cgaggttaat gctaatcacc gccataaagg tgaagaaact cagcaagccg tgggccgcct
     7921 gctgtcctgc catctggaat atgaggatcg gtcctcctat ggtctcggca ggtatgatgc
     7981 gttgaatgag ctttacgagg gaaaccacag tgagcgccga tatatcccat gtcttcatta
     8041 tcccgaaacc catggcttct gcaggaccaa acctctttat gaagtcattt ccaaacggcc
     8101 tcaccccgat caggcctatt tccttctttt ctcctccggg accggtaaat gtacttttga
     8161 caggagttat agtgaggtcc agaaatccat cgcccctttt tactctgaat ctcatcggtt
     8221 tgccaggatt gtcatagatc agtgaagtca tatcctgcca cgtcttaagg gctgagccct
     8281 ctatctcgag gatcctgtct cccttttgta tacccgcctt ctccgcagga gatcccttgg
     8341 tcacctcacc cacgtcaggg taaagaagcg gggaaatccc gatatcccag acctccctct
     8401 gctcgccgaa aacgtctttt tctgattttt tctcagggcc cacctgcacg tcaaccactc
     8461 gctcacccct tttgatggtg aagaggatcg tcttcccctg attcttgctg atggctgact
     8521 ctatctcatc ccaacccttg accgaagccc catcgatcgc caggacccgg tctcctgtca
     8581 tgagacccgc ctttgcagca ggggaacctt ccgatacctt cccaatgtca ggatacggga
     8641 caggcacccc ggacaccgcg atcagagcga atacaaaggc ggcaaacagg atattgaata
     8701 ccggtcccga gagtacgatg ataatccttt tccacacaga ctgaaaattg aaggcccttg
     8761 ccttctcttc atccgtaatc tcgtccccct gttcttctcc cagcatcttc acatacccgc
     8821 ccagggggac tgcggaaaga agatactcgg tttcgccgta cttcctccct aaaatcctgg
     8881 gaccaaaccc caacgagaac ttgagcacct tcacattcac cagcttcgcg aaaagaaaat
     8941 gccccagttc atgcacaaaa ataagtatgc ccagcagtat gattgcagaa aaaaatgtca
     9001 tcgtgactcc ttaagcggca aatttaaaaa taaaatcggg gaacgcctct cttctgtatt
     9061 cctgaggtta tgtccttgct ctcctaatgt gctcctctgc tttttccctt gcccatttgt
     9121 cgacttcgat gaccgcgcca agttccacgg cctttcctct tgtatgggaa ttcatcaccc
     9181 tcttaattat aacagggatt ctcccaaaat taattctctt ctcaagaaac gcattcaccg
     9241 cgacctcgtt caccgcattc agcaccgcag gcattgtccc cccttccctc agcgcatcgt
     9301 atgccagctg aaggcaggga aaattctttg tgtctggttt gtgaaacgtg agttgctgga
     9361 tgctggcaag ttcaaggcgt gctacggtac tggcgagcct ccggggatag gaaagggcat
     9421 aggcgatagg ccctctcata tcaggcacgg atatctgagc aaggagggcc ccgtcacgga
     9481 actccaccat ggaatgcaca atgctctgcg gatgcaccac cacatctatc atttcgtggg
     9541 gaaggcagaa aagatgggaa gcctctatca cctccagccc cttattcatc atggtggcgg
     9601 aatcgatggt gatctttttc cccatactcc agtttgggtg ttggagtgct tcctcggggc
     9661 tgacctccct caattccgct ttctttctcc ccatgaacgg gcctccggag gcagtaagga
     9721 taattcgttt cacgtacctc ctgtcctgac cctcaaggca ttggaagacc gcactgtgct
     9781 cgctgtccac agggagtatt gttgccctgt gtgcctttgc ttctttcatc acaatactgc
     9841 ctgcgatcac aaggctttcc ttgttcgcaa gtcctatgac ctttcctgcc cttactgcat
     9901 gaacagtggg caccaatccg ctgaagccga ccatggcaga gaggacaaaa tcggcctggc
     9961 ggcaggctgc caccgctttc atgccctcct ctccaaaaca gacctccgtc tttcttccca
    10021 ctgttctcct gagttccctg gcaccttctt ccttcgcaac cgccacgatc tggggtctaa
    10081 aggctttcac ctgccgcccg agaaggtcga catttttccc cgcagcaagt cccaccacac
    10141 aaaaatcgtc actatgtctt gaaattacct caagggccat tctcccgatg gagccggtcg
    10201 aaccaagaat taccacacgt ttcatttcat cccccaaaaa tttcggaaac cagataaagg
    10261 accggccccg cgaaaagcac accgtcaatt ttgtcgagta tccctccgtg cccggggact
    10321 atgccgctcg aatccttgac cccggcatca cgcttgaaca tggattccac gagatctcca
    10381 atgatggtaa cagtcccaat gattccgccg acaacgaagg actctcctgt ccccatagag
    10441 tgcaccagga ccatattcag catccatcct gaaagagaac ctcctatcag tgagcccacc
    10501 gcgcccgcta tggtcttgtt cgggcttatc tccaggtaga gtttcctctt tccaatgctt
    10561 ttccctacat aataggcagc gctgtccgag atccacacac agccatagag aaaaattatc
    10621 cactggggtc cgctgtgtct gagaagaacc tggaacgcga ggagtcccgg aatataaaag
    10681 agcgacaaaa gggtaaatga tatatcccga aggcaagacg aagggttcct taagccgaaa
    10741 agtcgtatgg cgaaaattac catgaccatg agcatggata cagggggaaa tggatccccg
    10801 gatatatacg cggccgtaag catcagcact ccaaagagta tcccggagtt cttcataaga
    10861 ccggtgaagc ggtacatgga gtaaagctcc cattgtgcga gagtacacgc aataaggagg
    10921 agcgcaaaga agcaccattc agggagtccc atgacggaga aatagaaaag ggggagaagg
    10981 agtactgcca ctaccagtct cttggcgtgc attctaggat tctgcccttg cctgcacagt
    11041 gccgaatctc ctctccctca tctggtagtt atgcagggca agaaaaaact cttccttcgt
    11101 gaaatccggc cagagggtat cggtgaaata aaactcagcg tatgcggcct gccagagaag
    11161 gaaattgctt atccgttttt caccgctcga tcttacaata agatccggcg cagggattcc
    11221 cgcggtgtca agcagggaac caaaatattc ctctgtgaca tcctcctcct ttactcccag
    11281 actgattatc ttcttcaccg cccgtatgat ctcattccta ccgctatagc tcagggcaag
    11341 cacgagggtc atgcctttgt tgtgggatga cttctcttcc gcattcttta agagggcctg
    11401 taccgacgcg ggcaaccgcc atacctcgcc aatagtcctg aatacaatgc ccctccttat
    11461 gagttcgctg aactccttac tgaggtaaaa ctcgaggagc ttcatcagcg tggacacttc
    11521 gtcatcgggc ctctgccagt tctcaaggga aaatgcatac agcgtgaggg tgttcacccc
    11581 cacttccatt gcggccgaaa taacctccct tgccctctct gcacccctct tatgtccctc
    11641 gatccttgga agaccgcgca tctcggccca cctaccattc ccatccatga tgatcgcgac
    11701 atgttcaggg attcttccgg acagcaacaa gttgtggctc ctccctttac attcctttaa
    11761 gggaataaat gtagagcatg cttcccaggg gattctcacc cttcatgatg ttcttgaatt
    11821 ttattccaaa caggggcttg ctgagcacaa tgagcttgtc ccctgcaaag gcataatcct
    11881 gaattgcacc ggaaatccct tccaccaggg tgttctcttc catggagaag ccggtccaca
    11941 agagtgtttt tatctgcgaa ctcttgtatc caagtccctt tgccacactt gcaaggggga
    12001 tccttttcac tgcaaaggag tccctgtttc tcacaaataa ccgatccttt attgaccata
    12061 cgcctctgtc taccataacg gttggcgcgg ccttcttaaa cgtggtgagc attcctccgt
    12121 agttgtcatt gctccgccat atcctcagtc ccatctcgtt gtaaagatta aggtatcctg
    12181 aatcgtcata tgcaaggaca ttctttgccg ccccggaacc atccacatac acgaaatcat
    12241 agatattcac ccccttggga agctttatct cttcacctgc cctgacctca cctgagctat
    12301 ataccatccg gaagacagga ccgccgaacc cctctgcgct atcaaacttt tgcgcaatga
    12361 gacctgtctg ggggagggac ctcaaaaaga cattcccctt ccagagcagg gaaagttcag
    12421 aacccttgag cgcatagatg tatgagacga cttttccttc gtccttaacg gcctgcacca
    12481 aagaatcgct cgtggtgtcc acacttcttc ccctgagaga cgtcacgata atctcatcct
    12541 tgccgtctcc atccacatcc gcagtatcga tccagagaaa atcttccgag gcggcgacct
    12601 tcagctcata gagatcctgg agaccagaac caagggaaaa tacccggaga tctctccctg
    12661 tgctgagaat tacctcgttc gttccgtctc ccttcagatc accgacagaa atcagttttg
    12721 cgccaaaagg aaggtcaaag gagaggaggg tatcgcttgc ggaagcgata ggggcaacca
    12781 tggtccgtga acccccgagc gccttcgcgt aagctgcctc aaccttcact tcattctccg
    12841 caggtcccga tgagtcttct acccaaagga ttctctgttt caggaacatt tcattgtcct
    12901 gctcccttcc cgtaaccgcg aacaccacgg ctgcgctcac ttttcttgcc tcagcgagca
    12961 acgcgtcatt gtccgcggaa ttcagggacg catccacgat ctcaaacctt ccgctctcct
    13021 tgagcaaatc gtaataagag tcggccaggt tccagttcgc actcttgtcc tggtaaaaga
    13081 gcacccggat tttcatttcc gagatgcgca caatatcacc caggctcgcg tcgcccttga
    13141 tgatctccat ggcggaactc ttctccttga cctcccttac cacggccttt ccttcaggcg
    13201 tttcggttct gcccaggggc tcctttgtga tgggatgcag gaagggggtt ccctctttga
    13261 gaatggtaaa tctcatgcct ttctttaccc ctgctgaaga accaaggtca gaggtgatga
    13321 ccttccccat cacggcaacg accttacctt taagcggcgt gaaatacgag agcgtctcgt
    13381 ctttcagctg gcgcagaggg tcctgggcgt gggctgcacc ggaaagaaga aacagaagac
    13441 cgatcacgga acacagcgca tatacagaat gcatggtccc ccttttccct cctctttttt
    13501 tcccgctgtg ttctgcgtcc tgtcgcctca ttttacctcc cagaagtcta tactaaaccc
    13561 taacctcttg tctccgttaa aatcaagtct ttttacaaaa aaaggctgtt ccctgacata
    13621 tcctcccgat gcctccttct aaacagagct tgtttccacc ggataatgcc acaaccctgt
    13681 ctcttctcca aaggaaaagg cgtatccaag attcaggcag ctatcacaat acatgccccc
    13741 accctccatc gcgccatgga gtctgcagaa atgacatgcc ggcgattatg gatcgccggg
    13801 gattttttcc ttcttacgtt tcttttgtga atttgtagaa tttaaaatta taccataccc
    13861 gttgctacga accccaaacc ctgaatgaat acgaatcact ccattgcgtg ctttctcctc
    13921 atacgtacgc tcactactta ttgcatttat gggtgtgata caataaaatt ctatgaggat
    13981 ttcgctcatc atacccacat tcaatgccag ctcgcacatc gaggaccttc tctcaatgct
    14041 ctgtgcacag gacaccaaac catgggaaat aattattgtc gattcctctt ccgaagacac
    14101 tacagtagaa atcgcgagga gattcggcgc aagaataatg gtaatcccaa ggcatgcctt
    14161 caaccacgga aaaacgagaa atatggcggc catggaggca aagggagaca tcctcgtttt
    14221 catgacacag gatgcattcc ctgaccagaa tactctcctc agcaccctga ccgctcctct
    14281 tcacatacat gatatcgcgg cttcttatgg aaggcacctc cccggaacga atgcttcccc
    14341 cctggagata tttgcgcgca ggttcaacta tcccgattcc cccatgacaa agtccttgaa
    14401 tgacatacag aaatatggca tcaagacgtt tttcttcagc aatgcgtgtt cggccattaa
    14461 gagagacctc ttcttgagcg ccgggatgtt tcgggagggt gtcagggcaa acgaagatat
    14521 gctcatggct gcacggttga tcctgagcgg gtatagggtg gcatacgttc ccgaagcaac
    14581 ggtaatccat tcacataact attctctctt gcagcagttc agacgctatt ttaatatcgg
    14641 ttcatccctg aagaataacg cctgggtcct cgagtatgcc cgtcccgaag gagaaggtct
    14701 ccggttcata agagggcaac tcggttttgt ggtgaagcat catggatacc tctggatacc
    14761 ctacattttt ttggagtctc tggcaaaata tacggggtac agggcgggac taatcgcagg
    14821 atgatacaga tcgtgaaatt attcagaaga cttattaagg tcgcctgcct cattcttatc
    14881 gacctcattt ccttctacct ctcccttttc gttgcctgga cagtgagaac ggaaatcctg
    14941 tcatcgttga tgtcaaatct gcccggattc ccctttccct atctctacta tctctccctg
    15001 tggtggatgc ccctcatctt catgttcttc ctgttatatg agggtctcta tgagaaaaac
    15061 ctcccgtttt gggatgaggc gcgcattacc ctgaaggccg tttcccttgc gtccctcacc
    15121 attatgacca tcgtcaccct tggaaaaatg caggacaagg tatcacgtat tatcctcctc
    15181 ggtctgtgga ccgcctccct cttcatcttt cccctgttaa ggctttgggg caagaaatgc
    15241 ctctatcaca taggggtatg gagggagagg gtgctcatac tcggtgcagg gaacgccggg
    15301 agacttacca tggaagggat ggaaagagaa agacatatgg gatacgaggt gatggggttc
    15361 ctcgatgatg atgaacagaa gagggggaaa gtcttcaaag ggaaaaaggt gttcggaagg
    15421 atcaagcact tccctaagtt tataaaggaa ctgaacattt ccaccgtgat catcgcaatg
    15481 ccttccctct ccccggaaag gctttccaac ctcaccgcca atgtccagaa ccattccccc
    15541 gatactatgg tgataccgga tctcaagggc attgcgctct tgaatacaga gcttctccat
    15601 ctcttctatg aagaactctt tctgatgaat ataagaaaca acctgaaatc ggcccccaac
    15661 aggttgataa agagaatgtt tgacgtgctc ttgagcactg ccatgatgcc tcttcttctg
    15721 ccccttatcg gaatcatcgg ggcggtgata cgttgggaga caccgggccc cgccatttat
    15781 tcccacgaca ggatcggcaa gaacggcaag accttcaggt gttacaaatt caggactatg
    15841 cagagagacg cggaggagaa gctcagggag atcctggaaa aggatgaaga aatccgcagc
    15901 gaatgggagc gaacatggaa gctgaaagaa gatccgagga tcaccagaat cggacgcttc
    15961 ttaagaaaaa cctccctcga cgagcttccc cagatattta atgtgatgag gggagagatg
    16021 agcctggtcg gtccgagacc ttatctgctg agggaacagg aaacccttga aagtcagctg
    16081 cacgtcatct gcagtacaaa acccgggatc acgggactgt ggcaggtttc aggaaggagc
    16141 aatacaggct atgaacacag gctgaaactt gatgcatggt atgtgatgaa ctggtcctta
    16201 tggtttgaca tcgccatcct cttcaagacc atacgggtag tgacgaagag ggaaggggcg
    16261 tactgaatgc atgggcgccg tatatttgtg atataataat gaagcaattt tactgcattg
    16321 taagaggagt gtaatccttg attcaggaca agacctatct ggcgttagag gatcttccaa
    16381 aagttccctt cgtggttgag gggatctctt cgcggtttat ccgtgagtgc atggtcattc
    16441 ctctggagct taagaacaat atccttaagg taatcatggc aaaccctgaa gacagagata
    16501 ccattgatgc cctcaaagtg gcaacctcat ctaatgtcct cgtctacacc gcggacccca
    16561 aggttgtgga tgaatacata tcaaagttct atggtcagga atcacagaac atagacaaga
    16621 tcatggagga catgggggat aagactcttg atttcaccag ggaggaagat gaggacacag
    16681 gacaccttaa agaccttgcc gccgaagccc ccatcatcaa actcgtgaac ctgcttatta
    16741 caagggctgt ggaaggaagg gcgagcgaca ttcatatcga accttttgaa gacgaagtaa
    16801 aggtacgata ccgtattgac ggagtgctgc atgacataga gtccgttccc aaaagacttc
    16861 aggcagcgat tgtctcgagg ataaaaatca tggcgaaact caatatcgcg gaacggagac
    16921 tgccccagga cggtcgtatc aggctgaagg tgggagaagg ggaaatcgac ttcagggtat
    16981 ccacaatccc tgtgctctac ggcgaaagtt tggtgatgag gatcctgcga aaggaaggaa
    17041 ttgtgataga ccttgacctt ctggggttcc ctccgaagac cctcgctgac ctcagtcagc
    17101 tcatcaccaa acccaatgga attatccttg ttaccggacc aacgggaagc gggaagacca
    17161 ccacccttta tggtgctctg gacaagatca attcccctga caaaaagatt atcactgttg
    17221 aagatcctgt ggagtatcag ctcaaagggg tgaatcagat acaggtcaag ccccagatag
    17281 gactgaattt cgccactacc ctgaggcaca ttgtgcgaca ggatcccgat gtcatcatga
    17341 tcggggaggt gagggacctg gaaacagccg aaatcgcaat acagtcagcc cttaccggac
    17401 atctcgtatt ctccaccctc cataccaacg acgcgcccag tgccattaca aggcttattg
    17461 atatgggcgt ggagaacttt ctcctctcct ccaccgtcag ggggattctc gcgcaacggc
    17521 ttgtcagggt agtctgccct gaatgcaagg aaagggattc ttcctcggcc gacagggagg
    17581 ccctgggcgc ccttggtatc gggagcgacg ttcctctctt caaggggact gggtgcgaaa
    17641 agtgtgcgtt caccggcttt tacggaagaa ccgggattta cgagctgctc attgtcaatg
    17701 atgaaatgcg gaagctggta ttgaaaggtg cggacgcaaa ccagctgagg gaagtggcca
    17761 tgcgtcttgg catgaagacc ctgctccaga gcggtaccga aaaaataagg gccgggatga
    17821 ccactctcag tgaggtccta agagtgacac aggaggcgta gttggccacc ttttcctaca
    17881 gggcaaccaa catggaaggc gccatcgtgg aaggggttat cgaggcagca gatgagaaag
    17941 ccgctatcga aagcctgagg aacgtcgggg tcattccgct caaggtcgcc gcccccggag
    18001 agggcatggg caaagccttc ctctcactca aacgatcgaa gggagacatc ctcaccttca
    18061 ccacagaact ctctgcgctc ctaagtgcgg gactacccct ggacaggagc ctcaatatca
    18121 tctccgagat ctccgagagc aaagagatga aaggtatcgt ccaatccatt ctcaagtcca
    18181 tcagagaggg acattccttt tctgaagccc ttcagaaaca tccgagaacc ttcccgaaaa
    18241 tttatgtaaa tatgataagg gcgggtgagg cagggggcgt ccttgatgtg gtgctggata
    18301 agctgaacga attcctcgag tcatcaaagg aactgaagga acatatcttc tctgcgatga
    18361 tctatccatc gatactcatt gtaaccggcg ggatctccgt catcgtcctg ctcacctatg
    18421 tgcttcccaa attctcggtg atattttctg aaatgggggc ttcactccct cttccgactg
    18481 cgatcgttct cggcttcagc agctttctcc aggcatactg gtgggttatg cttctcgccg
    18541 tcgtctcggg atggctcatc ttcaagaatt acataaagtc cccttccgga agataccaat
    18601 gggattcctt caagcttaaa ctgatgggtg aagtcataag aaagattgaa acggcccggt
    18661 tctcccgaac cctgggcaca ctcctgagaa gcggggtccc tcttctccag gcgctgaaca
    18721 atgcaaagga cgtgataacc aatcaggtca tcgcttctgc catcgacaag gtctcgaagg
    18781 gtgcaaagga aggaaagggc atctcggttc ccctctcgga tgccaatgtc tttcctcccc
    18841 ttgccctgtc catgatcaag gtcggagagg agaccggaca gctcgacgaa atgctgatga
    18901 aggttgcatc cacgtatgaa aaaagtctca gggtcgccat aaagcgcttc gtgagcttca
    18961 tcgaacccgc catgatactg gggatgggac tggtaatcgg cttcatcgtg gtctcgatgc
    19021 tcatggccat attcagcata acggaattgc cgttctaatg tgtacgggac atgaaatgcc
    19081 accgtgcact ccttccctca cttctctggc ccccgggaaa gaggcagacg caggggccgg
    19141 attcactctt ctggaactcc tcatcgtgct tgcccttgtc accctcgttg tgggaatctc
    19201 gactgtattc cttgtgaaca cgctccccgc gagcaagctg aatgcaactg cacgtgacat
    19261 atcttccaca atcaggtacg cgcggaccct cgcacagacc aacaatgagc aacaggtcat
    19321 gactattgac ctagactcaa accgctatgg aatagaaggt cgtggcgata gaaacatccc
    19381 tcccggcatg catatcaagg tgatggaccc tttctcgggc gagatccgta acgggacata
    19441 tcagatgaga ttccacgcgt ttggcacagg gggagcaacg gtcgtcgtgt ggaatgacaa
    19501 aagatctgtg agcatacagg tggatcccat aacagggccc gtgataacaa aatgagtgga
    19561 atggtaagat ccaaagggag ggtttccgga ataatccttt tcgcaccggg agtccggaga
    19621 ttcagaacct tgagctcttc cgcaaaaggg tttaccttgc tggaggtcct tgttgcggtt
    19681 gcccttctcg gcatcgcgat caccgtggtg ctgcaactct tctccgctga cctcaaggcc
    19741 attgcggcat ctgaagagta tgtatccgct actgcaaggg ctgaggcaaa gatgagggaa
    19801 gtgcaggaca atgacactct gtcagagggc accacgagtg agatgaccaa tgacggatat
    19861 aggttggatg tctcggtggc cagcacctta caagagagga ccgacaccct ccaggtaatg
    19921 ctcatggata tactcgtgac cgtacactgg accaagggca caa
//
LOCUS       DATKOU010000046        21958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4680360, whole genome shotgun sequence.
ACCESSION   DATKOU010000046 DATKOU010000000
VERSION     DATKOU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 21958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21958
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4680360"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VMU21_02045"
     CDS             <1..327
                     /locus_tag="VMU21_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76332.1"
                     /translation="DTDPQSGDRSAASPCADGRPYPQRPQADHGRLRRGRPVVDRNAL
                     GKKADVYVAFLEALDAYYGPVYCLGRLKEFTHYFARNYKFGHSLASKVQSSSSVDEAS
                     ERAGTF"
     regulatory      586..668
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            779..1252
                     /locus_tag="VMU21_02050"
     CDS             779..1252
                     /locus_tag="VMU21_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76333.1"
                     /translation="MVKEEAIDYMEELFTRPLFKRWFLEFFSKMQQEGIEGAKRFWNL
                     SHDGDGGVPRTPELFEKLIDFYIILGFVPRQRHERALGENEKLKRENKVLTDILRELH
                     CSVYGNGWKQMQEVWEEVMDRQREMNRDMGDMFLAFLRQMKQNEKEGELVAVGLS"
     gene            1536..1724
                     /locus_tag="VMU21_02055"
     CDS             1536..1724
                     /locus_tag="VMU21_02055"
                     /inference="COORDINATES: protein motif:HMM:NF020542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HUO76334.1"
                     /translation="MKVKAVFAKDGDWWMAWTNDIPGAVTSGGTLEEARENFMSLIAE
                     MQKPSIAKREMIFEEIEV"
     gene            complement(1756..3096)
                     /locus_tag="VMU21_02060"
     CDS             complement(1756..3096)
                     /locus_tag="VMU21_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO76335.1"
                     /translation="MASLNEILLRIKSKEVDYSRYRFSGTESTAFMTFFDLAQELDGL
                     EDFSLLCVAIPKVFFNLEARLYLVDPRLNKLSLFSKTEGEGAGLHAPPPEEVSPREHP
                     YFTSNNSLVLTIRGKELLIDELPFETRDNVLGVLEIYPMGEKSSHRILFFEKYANRIG
                     FNLHNRFLVEKNIEHLRFIRSLVADIEHNVIAPNIVYRLYLKHLRRKISKNIEIEQRI
                     SSAKEPGEEIATEHLLAELNEVNRGLVDELENLEKHYQNMSLFLETLFRRSHFDKGRL
                     TLIKKPCNMKKNVVQPQLERYADQFRNMGIAIDDRLSGLPDEEVISVVDIGLMAQVYT
                     NLFSNALKYTEEVVTHDGERKKYISYGREEIRDFFGPGKDGIKYNVFNTGPHIPDEER
                     SRIFEDEYRGSNATDKPGTGHGLAFIKNAVEIHGGVVGYEPTQYGNNFYFILPK"
     gene            complement(3189..4049)
                     /locus_tag="VMU21_02065"
     CDS             complement(3189..4049)
                     /locus_tag="VMU21_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HUO76336.1"
                     /translation="MRERILETVKQIEGLRDGHYHYEALTRKAEYIESEFVSLGFKVE
                     RDEFWFHEKLHWNIIATPSGPDSTGEQVLIGAHYDAVVGSPGADDNASGVAVVLEAAR
                     VLGPRTGLQCVAFSLEEPQVDSMDFLIGSKHFVKRMQTGGKRYKAAFILESVGYVSHA
                     PESQLSPAFVPAPTVGDFIGVVGNRKAGAILEKFKDMASRHTPQLKVVTHRAPIRGFL
                     ALETRFSDHAPFWDAGYPALMITDTAMFRNPYYHTPHDTSDTLSPEFMSQVAEALIHM
                     VDELLEPPLP"
     gene            4092..5078
                     /locus_tag="VMU21_02070"
     CDS             4092..5078
                     /locus_tag="VMU21_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HUO76337.1"
                     /translation="MLPGVIYTTEWFIIFRMYDVLLSFGLIIAMGAGFRMLKLGGLDA
                     DSLRSAINVSVFNVFLPALCIRIIYTSAIDREALLVPATAWITILSGLFIALAVYALL
                     EKTLSIGPREKGVLLLAATFGNVTYLGLPVLTGLYGYEAAKYALYYDLLAATPLLWFV
                     GAPLASRYGEGKRMGIGDSFRTIASLPPLWGIGAGFVLNLGGIALPSFLLKTLGMLGD
                     LVIPLMIFTVGLALSLPKVRHAYAIIPAVTIKLGVVPFIAYVAAMVLGLEGTALASCF
                     IEGAMPTMVLSLLIASRFKLDESLAAFVIVVTTVLSFITLPFAMHFTHHLIR"
     gene            complement(5094..5582)
                     /locus_tag="VMU21_02075"
     CDS             complement(5094..5582)
                     /locus_tag="VMU21_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76338.1"
                     /translation="MNKKLFIGLLFLLLLVSVLYLLWPSDEARIRKLFKEGAAAVESR
                     DIDGVMSKVSFAYRDDYGVTYLYLKEILKREFDRLSDIQVEYGALKIKIIKKGGPGEE
                     RPSPGPPDMAVAEMDIRVVATAGTETGYILGDVKTPVHIRFTLEKERMKWLIVKSEGI
                     MF"
     gene            5704..6990
                     /locus_tag="VMU21_02080"
     CDS             5704..6990
                     /locus_tag="VMU21_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO76339.1"
                     /translation="MVKKTATAEIRNKLRLLNLLITFVIFSLLFVIVRYLYAPFYQFL
                     SFLPDTSLVLIVLFAMGLVVAGLYLSRVLSGQVIREIEDYSERLDSILAITRDIREEI
                     YGDILLDKIMTCSVTITGSDAGSILLVAENGLVFKTIKGFSEEEHRGKTIPKDAGIGG
                     WVFQHGEPVLIGDVTKDERYNSSLDEFRGYRPGSLLCVPLRTKASTIGVIELLSKKQN
                     FYDTRDTEVISYLADQAAASVEMTRFYDDQRNYEIHLTDILLEAIDRFMSEKRGHPKR
                     VAQYANSIARALNMPEERKRRLYFASLLHDIGFLKISTEESFEKEAYTVHPVIGYEML
                     KPINFYKDIAPYVLHHHERYDGKGYPEKLKGNDIPLESRIIAIAEAFDSMASKVSYKG
                     HVNFDVAINELLRNKGGQFDPELVDLFVREIKQPLE"
     gene            6987..7811
                     /locus_tag="VMU21_02085"
     CDS             6987..7811
                     /locus_tag="VMU21_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUO76340.1"
                     /translation="MRNVLPLIIAIMDPKILRNIRERLLREVSEIQLKPFSQKALGVG
                     AAGDKTFPIDKIAEDIIISELESSRLPLSLISEEYGLKEINGGGRRVLIDPIDGSKNA
                     IAGIPFYCTSLAIAEGDTIGSISVAYVVNLINGDEFRAEKGKGAFLNNERISSQEDDE
                     FYLIAYEAQVPGRDIPRIIPLLSKARKTRCFGATALDLAYLSCGAVSVLVNPSLSRSF
                     DFAGGWLLIKEAGGVFTDTKGGSLEGIDLGLEKSAPLLASGNQRLHDKALALLGRP"
     gene            7834..8535
                     /locus_tag="VMU21_02090"
     CDS             7834..8535
                     /locus_tag="VMU21_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUO76341.1"
                     /translation="MSVQQRMKGLTPTVPLWGPEIKFVLLDMDGTLLDKYFDDYFWEH
                     LVPEKYAEKHGTTFGKAKTDLMTKYKRHEGTLNWTDIDFWSRELALDIPALKEQIRHL
                     IEVHPHVEDFLRMLKRHRKKVSLVTNAHVKVLDLKMKKTNLGKYFDACITSFDLGHPK
                     EDLQFWERARERLGFDNERTLFIDDTVEILKTARAFGIRYILYKARGNSKEKPQRSGG
                     FPAITDFKELFHEGI"
     gene            8522..10141
                     /locus_tag="VMU21_02095"
     CDS             8522..10141
                     /locus_tag="VMU21_02095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HUO76342.1"
                     /translation="MKASELIMRCLEREGVQYIFGLPGEENIDLLEAVLSSQIQFVLT
                     RDERGAAFMANVWGRLSGRPGVCLSTLGPGATNLTTGIADAYLDFAPAVALTAQKATK
                     DLHKESHQYINTVSMFRPITKWNTRIEVPDRIPEIVRKAFKLSSMEKPGPVHIEIPEN
                     IPSLSLDEEPLEPLDIIYPEPSRQAIARAVEMIKRARQPLILAGNGVIRGNATAHLAQ
                     FSRKLRIPVTTTFMGMGAIPADDDLFISTTGLQARDYISCGFDRADLIIAVGYDPVEF
                     NPNYWDGKKEILHINFTAADVDKHYRATEVIGNIKDTLSLLTESLTDEKDPSYFIALK
                     GFAEKILEPTHRGFPLKPLRVIQGIRKSLGREDILISDVGAHKIWISRFYPAYSPDTV
                     IISNGLAAMGIGIPAAISAKLLFPGKKVIAAVGDGGFLMSLPELETARRLNTPFVCLV
                     FNDGGLGLIEWKERLRYHKDFFVKFNNPDFVKLAESFGVKGYRVSSEDELEPILKDAL
                     DQNVPAIIDCQVDYSENLLLSEKLGGLICPM"
     gene            complement(10199..10381)
                     /locus_tag="VMU21_02100"
     CDS             complement(10199..10381)
                     /locus_tag="VMU21_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HUO76343.1"
                     /translation="MGAPVVDMDLCIGCGSCVELCPEVFELRDDKAWVIGADKCNTCD
                     CQAASDSCPVEAIKFE"
     gene            complement(10404..10718)
                     /locus_tag="VMU21_02105"
     CDS             complement(10404..10718)
                     /locus_tag="VMU21_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUO76344.1"
                     /translation="METIDLKKLITFYPDRISREMLSDKPEMRIALMCLNEGQKLEPH
                     KAPLRLLMYCVQGRGTFIIGEERVEADEKTCILCDPMVPHGFEANKGEKLVVMAVVTP
                     VE"
     gene            complement(10813..12063)
                     /locus_tag="VMU21_02110"
     CDS             complement(10813..12063)
                     /locus_tag="VMU21_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO76345.1"
                     /translation="MPLFGELFLSEILKQPILDPKGEELGRLKDIVVVKAEPLPKVNA
                     LIVERKNNLFRIPWLALTLFNKRIISATMFTADLIPYDVNTDDLHAVRDLLDKQIVDV
                     NGVKVVRVNDIKLEGFDSEAVLIAVDVGVRGILRRLGVERKGEDFLKVFKATLPYNLI
                     SWNYIQPLHPKLTSISLTVPRQMLSELHPADIADIISQVSHHEGAAFLKNLDVDVAAE
                     TLSELSTEAQVEILRDMDTEKASDIIEMMPPDEAADVLGDLPAERAKEILEHIEKEEA
                     EDIQELLGHEEDTAGGLMTNEFIAYSQDMTVQETIERFREDAKEIETVYYLYLVDGKE
                     RLVGVISLRELLLSEPHIKLSEIMETKVKTVTPEADEKVVGNITSKYNLVALPVVDCD
                     GVLLGIVTVDDILGRILPTEAKRK"
     gene            complement(12063..12899)
                     /locus_tag="VMU21_02115"
     CDS             complement(12063..12899)
                     /locus_tag="VMU21_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HUO76346.1"
                     /translation="MEPRQSPKTLTLGYSPCPNDTFIFYALAHRKIDTGDLRFREMLL
                     DVETLNQKALRAELDITKVSFHAYAHLRDRYVLLHSGGALGRGCGPLVVTKGNVKMDD
                     LKGKRIAIPGRITTAYLLLQLYEPLLAGNVVVMSFERILDAVRTGSVDAGLIIHESRF
                     TYPDYGLSGILDLGQWWEKETGLPIPLGCIIAEKNLGADLINTIEVLVRKSVEYAFAH
                     RDRTKEYIKAHAQELADPVIEQHINLYVNQYSLDLGEEGQRAVNELLRRAEQKGILRG
                     GQ"
     gene            12982..13965
                     /locus_tag="VMU21_02120"
     CDS             12982..13965
                     /locus_tag="VMU21_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUO76347.1"
                     /translation="MGIDISAKKSKFPSTQGEIAASEKISHIIDFSSKYLHGKDTPLR
                     LSLLTFFSKGHLLIEDLPGLGKTTLAIAIAKILGLSFGRIQCTSDLLPTDITGLSIYN
                     KNTGEFDFHPGPIFNNIVLVDEINRATPKTQSALLEAMGEKQTTLEGKTYKLPNPFFV
                     IATQNPAEQFGTFPLPESQLDRFMLKIGIGYPPRGAERKILKVGSSREELYAIEPMMT
                     KDEVIHIQEEIGQKVVISDRILEYILDVVEATRTSTYFLAGVSTRGVLALTTVAKTNA
                     YFHGRDFVIPEDVKEVTEYTLPHRVIFKEEYENLDRGEIIRSIIEHIRVPA"
     gene            14082..14852
                     /locus_tag="VMU21_02125"
     CDS             14082..14852
                     /locus_tag="VMU21_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUO76348.1"
                     /translation="MGISGFFGRRNLLDIRVSVGLPDEIYARTSVPLKVTLHNNRRFL
                     PAFLIKVHLHDQRVLFPFVDAKAEGVTYLVTTFNERGIVRINDFHLCSVFPFNFFIRC
                     RKIERAIEVTVFPHAKRCELPGLSERERQLRGERSIDKAGYEAEIMTMRDYIKGDPIK
                     YIHWKATAKTGKLKTKELSTPSHQPVIIDMDVVPIKNMEERISCITYIILKLFRENIP
                     VGLKIKGTFYKPFKVAQGDGLKVNKINMLRALAVYDKE"
     gene            14839..16755
                     /locus_tag="VMU21_02130"
     CDS             14839..16755
                     /locus_tag="VMU21_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HUO76349.1"
                     /translation="MIKSSSPLRLDGVVKVLTYLIVAIGASSVVRYIGLFYPLAFLCI
                     FLLSLYLDYRRRFFVPRWLLTGAALSVIVITLFRARTDDLVDASLGALLILLGIKLLT
                     DKRFRDYMQIYLIVLFLLTGSSLLSLDIEFLFYFIALIFLVSIAMVLLTYCSQDSALE
                     MPVSSVRTIVSKSSLIPLFAIPMTLFMFIILPRTSYPLFYSLQRGSSAHTGFSDTVRL
                     GNVSDIQEDAAVVLRAHMERIDENSLYWRGIVLDTFDGISWQSMHKELPDKNRPPLLS
                     GKRIPQTIYLEPYGNKYLFALDKPSSISLRYVSGYRDLTYSFPFILSKMIRYETLSAL
                     SDVFVDPEIDRSVYLQLPGARLARTKELARSLSRGTQEETIQEMLNFLRNGTYLYSLE
                     NLPLSGNPLEDFLFNNKYGNCEYFASAMAVMLRMAGIPSRVVGGYRGGYYNDTGEYYL
                     VPQKNAHVWVEAYLESGRWKRLDPTPAGIEHFVSFTRENLFFKVRFFLDTINYYWNAL
                     VINYDFAKQMTLLQKLRSFKKPKFSFPVSKEQLLRYSAALLVFGLSVWLIYFLISKRR
                     APEEKLREAFIRKMGKYGYRKAPSEGLEEFVSRVEEAPLQEKAYRFVREFERFYYKDI
                     PLTREEAKRLKHLL"
     gene            complement(16794..17321)
                     /locus_tag="VMU21_02135"
     CDS             complement(16794..17321)
                     /locus_tag="VMU21_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HUO76350.1"
                     /translation="MKHILFIGDSLVEFFDWQQRFSGHTIANLGIAGETVEGLYSRIG
                     KIIRKFPSPDLILIMTGINNVAMEDLGFLDSYRKVVEALSSAYPRTRIVLHTLLPTLL
                     PWIPKETIREMNRLLHKIAQETHTEVLDLYNRFVDPQGNPIKDYLLPDGVHLSEKGYA
                     RWVEVLTRIIDKSET"
     gene            complement(17347..18141)
                     /gene="mqnB"
                     /locus_tag="VMU21_02140"
     CDS             complement(17347..18141)
                     /gene="mqnB"
                     /locus_tag="VMU21_02140"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HUO76351.1"
                     /translation="MSHQGPPFTDMIALISSVPEEGALLARQLKRKTLLAGKPVYKGR
                     INNKAVVHITSGMGKTNAAHAATILLEKFSPEMLVLFGIGGAYPSAGLTIGDIVVAEK
                     EVYGDEGVMLKDEFHGTEFIGIPLLRKRRRNYFNEFPLDKPLIRRAKNILSPLFFCPP
                     SENQGKKGLKEKRGECKIQSGTFVTVSTCTGTVKKALELEKRFGALCENMEGASVAHV
                     CAMYDTPVIEIRGISNIVSNRDKHKWDIRFAAANCREAVIALLKNL"
     gene            complement(18330..19586)
                     /locus_tag="VMU21_02145"
     CDS             complement(18330..19586)
                     /locus_tag="VMU21_02145"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HUO76352.1"
                     /translation="MDIKQYVYNLAVEARAGARALAKASSSQKNLALVKMAEALKERA
                     PELISENKKDIGYAEKKGLSKALIDRLTLNEKRIQEMAQGLLEVSQLPDPVGEVIKMW
                     QRPNGMAVGKMRVPIGVIGIIYESRPNVTADATSLCLKTGNAVILRGGSEAIHSNKAI
                     VKILRDTAREQGIHEGAVTFIDIPEREAIMEMLKLEGIIDLIIPRGGEGLIRTVTENS
                     RIPVLKHYKGVCHVFVDRDADLIMAEEICFNAKVQRPGTCNAMETMLVDEFIAAGFLP
                     KMLKRFKDAGVELRGCSKTLGIYPHIAQAKEEDYYREYLDLILNVKVVKDMDDAMEHI
                     AKYSSAHSDSIVTMNYDRAMRFLRQVDSSAVFVNASTRLNDGYQFGLGAEIGISTDKI
                     HARGPMGLEELTCTKFIVFGSGQLRE"
     gene            complement(19698..20192)
                     /locus_tag="VMU21_02150"
     CDS             complement(19698..20192)
                     /locus_tag="VMU21_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76353.1"
                     /translation="MSLALWLQIKDQYDYTVMYVGSELVTAFKDLNCEGISWKGKDTI
                     QATFRPLRISAEILIDVSIHPSAYTFNTPTTIGIMAALFPFIRRRRRAYIEALIIFFS
                     LHVLTVFSLEAAQLARELVARRVDETGGHISFNLFLRHIMGNLVTKSEPFLIGVYIYL
                     RFKA"
     gene            complement(20239..20802)
                     /locus_tag="VMU21_02155"
     CDS             complement(20239..20802)
                     /locus_tag="VMU21_02155"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HUO76354.1"
                     /translation="MAQGRKDNKSDSGERKRKTGEISIWSFLIKYFMLMGLLTFVLVY
                     KPLKERIDVNNVYSKAVVIVTAEALGISGISCTYRDSIISVPGSTLDVRFGCNGLEAV
                     MIYSAAVVAFPAPWKKRFIGVLAGFLVIQAANILRITALVFSAIHYKNFLDYIHNYVT
                     QGLMIAISLAVFFIYLTYAGNQKTLEE"
     gene            complement(20898..>21958)
                     /locus_tag="VMU21_02160"
     CDS             complement(20898..>21958)
                     /locus_tag="VMU21_02160"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HUO76355.1"
                     /translation="SIQVNDDSKTFLLVASGNTSGLAQTIFDDAPLLRSYKDATHNIQ
                     RDAFAWGSTVYARASGLNSSPCWQVKWIDPNNNVVATDNLDASTSSSRDDSFVVPASG
                     PSGIWRAELWRPDNNNACSSATFTGSNPRYTLYFDVAQIVVIGADDSYITNHSPDFNS
                     NSSLLFKYTLDVNNDSNDLEKTFVRFTLPILPGNVHSAKVRMRVLVPPSFFSSRTYNI
                     DRVTGAWDVNTITFNLQPGVAGTFTDQQSTPTGLSAINSLMRWDVTSDVKGFALGWFA
                     NNGWRIVDSQAQDPEGTFYSTEYNTILGSNSEFYGPVLLIDTDPFSIPTMTEWGMIIF
                     MVLAGLGSIFCLRKRKRI"
BASE COUNT         5472 a         5481 c         5384 g         5621 t
ORIGIN      
        1 gatacggatc cgcaatctgg tgatcgatcc gccgcttctc cttgcgccga tggcaggcct
       61 tacccacagc gccctcaggc ggatcatggt cggcttcggc ggggtcggcc tgttgtcgac
      121 agaaatgctc tcggcaagaa ggcggatgtg tacgtcgcgt ttctcgaagc cctggacgcg
      181 tattatggcc ccgtgtattg cttggggagg ctgaaggaat ttacccacta ctttgccagg
      241 aattataaat tcgggcactc cctggcatcc aaggtccagt caagcagcag tgtggatgag
      301 gcaagcgaaa gggccgggac gttctgataa ctggggacac atcccactat gaatttgcgg
      361 gaagaaatcg acgggagaat tttcccaggc gagaaaaagg gacaggttac ttcattggag
      421 tgattggagc aggtgattat gtgcggtctc gggctgaggc tggcaggtct ggccccgcag
      481 agagaattga ctctttttat ccttgtaggg aaaatgccta caaaaaattc agacctggac
      541 ttatagctgc ggcagtttat tgatgatata aggattaaga agatatcccg ataaaaccaa
      601 accctctgtg aagaggggac ggaacgcggc ggatcctggg ttagatttgg gatggccgag
      661 ttgccgaagg aggggacctt tggcgtgttc ggctaatttt cttataacac tacttcgcgg
      721 caagaggtca gacataccgc taccttcatt tcctatagga tgacctaaag ggggaacaat
      781 ggttaaggaa gaagcgattg attatatgga agaactgttt accaggcctc ttttcaagag
      841 atggttcctt gagttcttct caaagatgca gcaggagggt attgagggtg caaaacggtt
      901 ttggaatctt tcccacgatg gcgatggagg ggtccccagg acgccggaac tgttcgaaaa
      961 gctcattgac ttctatatca ttctcggctt tgtaccgcgt cagaggcacg agagggcgct
     1021 gggagaaaac gagaagctga aaagagagaa caaggttctc acggatatcc tgagggaact
     1081 tcactgttct gtgtatggaa acggctggaa gcagatgcag gaggtgtggg aggaggtcat
     1141 ggacaggcag agggagatga acagggatat gggggatatg ttcctcgcct ttttgagaca
     1201 gatgaaacag aatgaaaagg aaggagagtt ggtggcagtg ggtctctcct agccggtatt
     1261 attgatgagt ttctttgtct cgccaggggc atccgtctcc cggcaagaca attgccctcc
     1321 tcaactgtcc ggtgggctgt gcacctcaca ccactctgtt cttatgtagt gtcaagtccg
     1381 cctttgcgga caagcacgta acccgtcctc ctcaggcgga ttgccattgg agccggatgc
     1441 ctctggtgac tgtatgcagt atcaaggcta gtgtgattgc ggacgcccca aggatgtgct
     1501 acatttagag aacaagacaa gaaaggagaa tccctatgaa ggtgaaagcg gtgtttgcaa
     1561 aggacggaga ctggtggatg gcctggacga atgatatccc tggagcagtg acgtcgggtg
     1621 gaaccctgga ggaggcgagg gaaaatttca tgtccctcat tgcagaaatg cagaaacctt
     1681 ctatcgccaa acgggagatg atttttgaag agatcgaggt atagagcggg gaagcgttcc
     1741 ttaaagggga gatgattatt taggcaggat aaaatagaaa ttgttcccgt actgagtagg
     1801 ctcatacccc acaacgcccc catggatttc aacggcattt tttatgaacg caagcccatg
     1861 gccagttcct ggcttatccg tagcattaga gccccgatac tcgtcttcaa aaatccttga
     1921 ccgttcttcg tcgggaatat ggggtcccgt gttaaagaca ttatacttga tgccgtcttt
     1981 gcccggaccg aagaagtccc tgatctcctc gcgaccataa gaaatatatt tcttcctctc
     2041 cccgtcatgg gtgaccacct cctcggtgta cttgagggca ttggagaaaa ggttcgtata
     2101 tacctgggcc atgaggccga tatccacgac gctgatgacc tcctcatcag gcaagccgct
     2161 caagcggtca tcaatggcaa tccccatatt tctgaactga tcagcgtatc gttcgagctg
     2221 cggctggacc acattctttt tcatgttgca gggcttcttg ataagcgtga gcctcccttt
     2281 gtcaaaatgg ctcctgcgga agagggtctc gagaaagagg ctcatgttct gatagtgctt
     2341 ctccagattc tccagttcat ccacgagccc gcggttcacc tcattcaatt ctgccaggag
     2401 atgctccgtc gcaatttctt ctccgggctc ctttgcagag gagatgcgct gttctatctc
     2461 tatgttcttc gagatttttc ttctgagatg tttcaggtag agcctgtaca caatattggg
     2521 agcaatgaca ttgtgttcga tgtcagccac aaggctgcgg atgaatctca ggtgctcgat
     2581 atttttctcc accagaaacc ggttgtggag attaaagccg atgcggttgg catatttctc
     2641 aaaaaaaagg attcggtgag aactcttttc tcccatggga tagatttcga gcacaccgag
     2701 gacattatcc ctggtctcga agggaagctc gtcgatgagg agttctttcc cgcggatagt
     2761 gagcaccaga ctattgttgc ttgtaaaata gggatgctca cggggactca cctcctcagg
     2821 gggcggtgca tgcagccctg ctccctctcc ttcggtcttt gaaaagagcg agagtttgtt
     2881 gagcctggga tctacgaggt agagacgcgc ttccagattg aagaaaactt tcggaatagc
     2941 cacgcacagc agggagaagt cttccaatcc gtcgagttcc tgggcaaggt cgaagaaggt
     3001 cataaaggca gtactctcgg tgcccgaaaa ccggtagcgg gagtaatcca cttccttgct
     3061 cttgattctt agaagtattt cgttcaggga agccatgcct gctcctcatt aaggtgtcat
     3121 cgcatgctgc attctatgta cccattatac caaacacctt ccccttcttg aaggacggag
     3181 ccctctgctt aagggagggg aggttcgagg agttcatcca ccatatggat aagggcctct
     3241 gccacctgtg acataaactc cggagagaga gtgtcagagg tgtcgtgagg tgtgtggtag
     3301 taagggttcc ggaacatggc agtgtcggtg atcatgagcg ccggataccc ggcgtcccag
     3361 aacggggcat gatcgctgaa ccttgtctcc agagccagaa atccgcgtat gggcgccctg
     3421 tgggttacta ccttgagttg gggagtatgg cgacttgcca tgtctttgaa cttctccaaa
     3481 atagctccgg cctttctgtt ccccaccaca ccgatgaaat cgcccacagt gggggctggt
     3541 acaaaggccg gcgagagctg gctttcaggg gcgtggctta cataccctac tgattccagg
     3601 atgaaggctg ccttatatct cttccccccg gtctgcattc tttttacgaa atgcttgctt
     3661 ccaatgagga aatccatgga atcgacctgt ggttcttcca gactgaaggc cacacactgc
     3721 aatcctgtcc ttggacccag gaccctcgct gcctccagca ccaccgcaac cccggaggca
     3781 ttatcgtcag caccggggct gcccactacc gcatcataat gggcgccgat gagaacctgc
     3841 tctccggtgc tgtccggtcc cgaaggagtt gcgataatat tccagtgcaa tttttcatgg
     3901 aaccagaact catccctctc gaccttgaat cccagagaga caaattccga ctcaatgtat
     3961 tctgccttcc tggtgagcgc ctcgtaatgg tagtgaccat cacgaagccc ctcaatctgc
     4021 ttcactgtct caagaattct ctcgcgcatt acttccccgt tgtatccaaa cattctcaat
     4081 tgtgggtgac catgttaccc ggtgtaatat acaccacgga atggtttata atcttcagaa
     4141 tgtacgatgt gcttctttcc tttggactca ttatcgcgat gggtgcgggg ttcaggatgc
     4201 tcaaactggg cggtctggac gcggattccc tccgtagcgc cattaatgtc tctgtattca
     4261 acgtattcct gcccgcactc tgcataagaa tcatctacac ctctgctatt gacagggagg
     4321 cgctccttgt tcccgcaacc gcatggatca ccatactttc aggtctgttc atcgcccttg
     4381 ccgtgtatgc gcttctggag aagaccttat ctatcggccc gcgggagaaa ggggttctgc
     4441 tcctggcagc aacatttggt aatgtgacgt atctcggact tcccgtgctc accggattgt
     4501 acgggtatga agcagcaaaa tacgctttat attatgacct tctggccgca accccgttgc
     4561 tctggtttgt gggtgcccca ctggcgtcac ggtatggtga agggaaaagg atggggattg
     4621 gggactcctt cagaacaatc gcttcacttc ctcctttatg gggaatcgga gcgggatttg
     4681 tcctcaacct tggcggcatc gccctcccct cttttttgtt gaaaacgctg ggtatgctcg
     4741 gagaccttgt gatccccctt atgatcttca ccgtcggcct tgccctttcc cttcccaagg
     4801 taaggcacgc gtatgccatt atccccgcgg tgactatcaa gcttggggtg gtgcccttta
     4861 ttgcgtacgt ggctgcaatg gttttgggcc tggaggggac tgcactcgcc tcttgcttca
     4921 tagaaggggc aatgcccacc atggtgttgt ctctcctcat cgcttcacgg tttaaactgg
     4981 acgaatctct ggcggccttt gttatcgtgg tgaccactgt tttgtccttt attaccttac
     5041 ccttcgccat gcatttcacg caccacctga tccgttgaag agagacagtt tccttaaaac
     5101 atgatcccct ctgacttcac gatgagccac ttcatgcgct ccttctccaa ggtgaacctt
     5161 atatgaacgg gcgttttgac atcccccaat atgtatcctg tctcggtacc cgcggttgcg
     5221 accaccctta tgtccatctc tgccactgcc atgtcagggg gaccagggga gggacgctcc
     5281 tcccctggtc cccccttttt gattattttg atcttcagcg ctccatattc gacctgtata
     5341 tcggagaggc ggtcaaattc tctcttgagt atctccttga gataaaggta ggttacccca
     5401 taatcgtccc ggtaggcaaa cgataccttt gacatgacac cgtcaatgtc tcttgattcc
     5461 acggccgcgg ctccctcttt aaagagcttt cttattcgtg cttcatcaga aggccagaga
     5521 agatagagaa ccgatacaag aaggagtaaa aaaaggagcc ctatgaagag ttttttgttc
     5581 attccgcatt catattacca taatgagccg ctccccggtc cccttttaca tcccattcac
     5641 ttcatcatcc gtaaaattga cattcaccgg ttgttcatgt acatttgaat aatggctgat
     5701 gccatggtta agaaaactgc aactgcggaa ataaggaata aactcaggct cctgaacctc
     5761 ctgattacct tcgtaatatt ttctctcctc ttcgtgattg tcagatacct ttatgctccc
     5821 ttctatcagt tcttaagttt ccttcctgat acatcattgg ttctgatagt gctattcgcc
     5881 atggggcttg tggtcgcagg actatatctc tcaagggtgc tgtcagggca ggtcatacgt
     5941 gaaatcgaag actacagcga aaggcttgac agcattcttg ctattacgcg agacatcagg
     6001 gaagagatct atggtgatat actccttgac aagataatga cctgctcagt gaccattacc
     6061 ggatccgatg cgggctcgat actccttgtg gctgaaaatg gtctcgtctt taagaccata
     6121 aaaggcttca gcgaagaaga gcacagggga aagaccatcc cgaaggatgc gggtatcgga
     6181 ggatgggtct tccaacacgg tgaacctgtc ctgattgggg atgtaaccaa agacgaaagg
     6241 tacaattcct cccttgacga gtttaggggg tatcgcccgg gttctctgtt atgcgtaccc
     6301 ctgaggacaa aggcatcgac cattggggtt atcgaactcc tgagtaaaaa gcagaacttc
     6361 tacgatacaa gggataccga ggtgataagt tatctcgctg accaggctgc cgcgtccgta
     6421 gaaatgacaa gattttatga tgatcagaga aactatgaga tacatctcac cgacatactc
     6481 cttgaagcaa tagaccgatt catgtctgaa aagcgaggcc atccaaaaag ggttgcacag
     6541 tatgccaata gcatcgcaag agccctcaat atgccggagg aaaggaagag gagactttat
     6601 tttgccagcc ttcttcatga catcggcttt ttaaagatat ccactgaaga gagctttgaa
     6661 aaagaggcgt atacggtgca tcccgtaata gggtatgaga tgctgaagcc gataaatttc
     6721 tataaagaca tcgctccgta tgtccttcac caccatgaga gatatgatgg gaaaggatac
     6781 ccggaaaagc tgaagggtaa tgatatcccc ctcgaatcca ggatcatcgc catagcagag
     6841 gcatttgatt ccatggcaag caaggtatct tataaaggtc acgtgaactt cgacgttgca
     6901 ataaacgagc tcttgaggaa caagggtggg caatttgatc ctgaacttgt tgacctcttt
     6961 gtcagagaaa tcaagcagcc gttagagtga ggaatgtcct ccctctcatt attgctatta
     7021 tggatcccaa aatccttaga aatatcaggg aaagactctt acgcgaggtg tcggaaatac
     7081 agctcaaacc cttctcgcaa aaggcgctgg gagtgggagc cgcgggagac aagacctttc
     7141 ccatagacaa gattgctgag gatatcatca tttctgagct ggaatcctca cgccttcccc
     7201 tctctcttat ctcggaagag tacggactta aggaaataaa tggcggggga agaagggtgc
     7261 tcattgaccc aatcgacggc agcaagaatg ctattgcggg catacccttt tattgcacct
     7321 cccttgccat tgcggaggga gacaccatcg ggagcatctc cgtcgcctac gtggtcaacc
     7381 ttataaacgg ggacgagttc cgggctgaga aggggaaggg cgcctttctg aataatgaaa
     7441 ggatctccag ccaggaggat gatgaattct atctcatcgc gtacgaggcc caggtgcctg
     7501 gcagggacat tcctcggatt atcccgctcc tgtcaaaggc acgcaaaaca aggtgttttg
     7561 gtgcgaccgc cctcgacctt gcgtacctct cctgcggggc cgtaagcgta ttggtgaatc
     7621 cctccctttc caggagtttt gattttgccg gcggatggct ccttattaag gaagccggag
     7681 gggtctttac cgacaccaaa ggtggttccc ttgagggaat tgacttaggg ctggaaaaga
     7741 gcgcaccgct tttggcatcc ggaaatcagc gcctccacga caaggcatta gcactccttg
     7801 ggagaccgta agctgcgcga gaagggaacg gacatgtcgg ttcagcaaag aatgaaaggt
     7861 cttacaccca cagtcccctt gtggggacct gagattaagt tcgttctcct tgatatggac
     7921 gggacactgc tggacaaata ctttgatgac tatttttggg aacaccttgt ccctgaaaaa
     7981 tatgcggaaa agcatggcac cacctttggt aaagcgaaga ccgacctgat gacaaaatac
     8041 aaacgccacg aagggacact gaactggaca gacatagact tctggtcaag ggagcttgcc
     8101 ctggacatac cggcactgaa ggaacagatc aggcatctca ttgaggtgca tccccatgtg
     8161 gaagatttcc tcaggatgct caagaggcac aggaagaaag tctcccttgt cacgaatgca
     8221 catgtgaaag tcctggacct caagatgaag aagacgaacc tcggaaagta ttttgacgca
     8281 tgtattactt catttgatct gggacatccc aaggaggatt tacagttttg ggagagggcc
     8341 agagagcgat tggggtttga caatgagagg accctcttca ttgacgacac ggtggagata
     8401 ttgaagaccg caagggcatt cgggatacgg tatatcctct ataaggcaag ggggaattca
     8461 aaggaaaaac cgcaaaggtc agggggattc cctgcaataa ccgactttaa ggagttattc
     8521 catgaaggca tctgaactta tcatgcgctg tctcgaacgt gaaggcgtcc agtatatctt
     8581 cggccttccg ggagaggaga atattgatct cctggaggcc gtgctctcgt cacagataca
     8641 atttgttctt acaagggacg aacggggcgc tgcatttatg gcaaatgtct gggggaggtt
     8701 atcaggaagg cccggagtct gtctctccac cctcggaccc ggggcaacga acctcaccac
     8761 aggaatcgcg gacgcgtatc tcgattttgc gccggcagta gccctcacgg cacagaaggc
     8821 aaccaaggat cttcacaagg agtctcatca atatattaat accgtatcaa tgtttcgccc
     8881 cattacgaag tggaacacca ggatagaggt gccggacagg attcccgaga ttgtccgcaa
     8941 ggcatttaaa ctctcttcca tggaaaagcc gggacctgtg cacattgaaa tacctgagaa
     9001 catcccctcc ctctccctgg acgaagaacc ccttgaacct ttggacatta tctatccgga
     9061 gccatcgcgt caggccatag cccgtgctgt tgagatgatc aaaagggcac ggcaacccct
     9121 catccttgca gggaacggag tcataagggg aaatgcgact gcgcaccttg ctcaattctc
     9181 gaggaaactc aggataccgg tcaccaccac cttcatgggg atgggtgcaa tcccggctga
     9241 cgacgacctg tttatttcca ccaccggatt gcaggcaagg gattacatct catgcgggtt
     9301 tgaccgggct gaccttatta tcgcggtggg ctatgacccg gtggaattca atccaaatta
     9361 ttgggatggg aagaaagaga tcctccatat caattttact gcagcggatg tggacaaaca
     9421 ctacagggca acggaggtta taggaaatat caaagacacc ctttccctcc tcacagaatc
     9481 cctcaccgac gagaaagacc cctcatattt catcgcctta aagggatttg ccgagaagat
     9541 actggagccc acccacaggg ggtttccgtt aaagcccctc agggtaatcc aggggataag
     9601 aaagagtctt ggaagggagg atatactcat cagtgacgtg ggggcgcata agatctggat
     9661 ctccaggttc tatcctgcct atagcccgga tacggtgatt atctccaatg gtcttgctgc
     9721 aatggggatt gggatccctg cggcaatatc cgcaaaactg ttatttcccg gtaaaaaggt
     9781 gatcgccgca gtgggtgacg gagggtttct catgtcgctg ccggaattgg agactgcccg
     9841 gaggctgaac acgccctttg tgtgtctggt attcaatgac gggggattgg ggctgattga
     9901 atggaaagag cggttgcgtt accacaaaga cttttttgta aagttcaata atccggattt
     9961 tgtaaagttg gcggaatcct ttggggtgaa gggataccgg gtctcttcag aagatgaatt
    10021 ggaacccata ctcaaggatg ccctggatca gaatgttccc gctattatcg attgccaggt
    10081 ggactattcc gaaaatctgc tcctttctga gaagctggga ggattgatct gccccatgtg
    10141 acgccaaggg cgcgggataa aatcaccccg cgcccctggt cgtatgtagt cccggaagtt
    10201 attcgaattt gatcgcttcc accggacagc tgtccgaggc agcctgacaa tcgcaggtat
    10261 tgcacttgtc ggcgccgatg acccatgctt tgtcatccct gagttcgaag acctcagggc
    10321 ataactcaac gcatgatcca cacccgatgc ataagtccat gtccaccact ggtgctccca
    10381 tagtatccct cctttatcaa gtattattcg accggcgtta ccaccgccat cacaaccagt
    10441 ttttccccct tgttcgcctc gaacccgtga gggaccatgg ggtcgcagag tatgcaggtc
    10501 ttttcgtcag cctctactct ttcctcacct attataaatg tccccctgcc ctgaacgcaa
    10561 tacatgagaa gccgcagggg ggccttgtgg ggttccagct tctgcccttc attgaggcac
    10621 ataagcgcga ttctcatttc cggcttgtca gagagcatct cccgggaaat cctgtcagga
    10681 taaaacgtga taagcttttt cagatcgatt gtttccataa ggcctccttc atgcttttct
    10741 ctcaggatat gattatgaga gccactttca ccatgacaca cgtcataccg cttccttctc
    10801 ctctgcgcgt ctctactttc tctttgcctc ggtgggcagg atcctgccaa gaatatcatc
    10861 aacagtcacg attcccagca agaccccgtc gcaatccacc acaggcaagg cgactaggtt
    10921 atacttcgag gtaatgtttc ccacaacctt ttcatccgcc tcaggggtaa cggtcttcac
    10981 ctttgtctcc atgatttccg acaacttgat gtggggctca gacaggagca gttccctgag
    11041 ggatattacc cccacaagcc tctctttacc atctacgagg tagagataat agacggtctc
    11101 gatctccttg gcgtcttccc tgaatctctc gatggtctcc tgtacggtca tgtcctggct
    11161 ataggcaata aattcattgg tcataaggcc gccggcagta tcctcttcat gaccgaggag
    11221 ttcctggata tcctctgcct cctccttttc aatatgttcg aggatctcct tcgccctttc
    11281 tgcggggagg tcgcccagga catctgcggc ctcgtcaggc ggcatcatct cgataatatc
    11341 cgaggccttc tctgtatcca tgtccctcag aatctcgacc tgagcctctg tactcagctc
    11401 ggaaagggtc tctgcagcca cgtccacgtc aagatttttc agaaatgcag cgccttcatg
    11461 atgggacacc tgactgatga tatccgcgat gtctgcaggg tgaagctctg aaagcatctg
    11521 ccgtggaact gtgagggaga tggaggtgag cttgggatgc aggggctgga tataattcca
    11581 gcttatgaga ttgtaaggaa gggtagcctt gaagactttg agaaaatcct cgcctttccg
    11641 ctcaaccccg agtctccgta atatccccct gacacccaca tccactgcaa tgagcactgc
    11701 ctctgaatcg aacccctcaa gcttgatatc attcaccctc accaccttta cgccattgac
    11761 gtccacgatc tgcttatcga ggagatccct caccgcatgg agatcatcgg tattcacgtc
    11821 ataaggaatg agatccgcgg taaacatcgt cgctgaaatg attcttttat taaagagggt
    11881 gagtgccaac cagggaatac ggaacaggtt gttcttccgt tcaacgatca atgcattcac
    11941 cttgggcagg ggttcagcct tcaccaccac aatgtctttg agcctgccca attcttctcc
    12001 tttagggtcc aggatcggct gtttgaggat ctcgctcaag aataactcac cgaacaatgg
    12061 cattattgtc ccccccgtag tattcccttt tgctcagccc gtctcaataa ctcgttcact
    12121 gcacgctgcc cttcttcgcc cagatccagg gaatattggt tcacatataa attgatatgc
    12181 tgctcgatca ccggatcggc aagctcctgg gcatgtgcct ttatgtactc ctttgtcctg
    12241 tctctgtgcg caaaggcata ctcaacactt tttctcacaa ggacttcaat ggtattgatc
    12301 aggtccgcac cgaggttctt ctctgcaatt atacacccaa ggggtatggg aagtcctgtc
    12361 tccttctccc accactggcc gaggtccaga atccctgaga gtccgtaatc aggataggtg
    12421 aacctcgact catggatgat gagccccgca tccacagaac ccgtccttac ggcatccaga
    12481 atcctttcaa acgacatcac caccacatta cctgccagca gcggttcata gagctgaaga
    12541 aggagatacg cggtggtaat cctccccggt atcgcgattc ttttcccttt gagatcatcc
    12601 attttcacgt tgccctttgt gactacgaga ggaccgcatc cccttcccag ggcgcctcct
    12661 gaatggagga ggacatacct gtccctcaaa tgcgcgtagg cgtggaacga gacctttgtg
    12721 atatcgagct cggcacgcaa cgctttctgg ttcagcgtct ccacgtcgag cagcatctcc
    12781 ctgaacctca aatcacccgt atcgatcttc cggtgcgcaa gggcatagaa aataaaggtg
    12841 tcattggggc acggggaata cccgagggtg agggttttgg gggactgccg tggctccatt
    12901 agggcctcct cttacgatac aaaagacata tattagtata cttaaaaaat cgtaaagtga
    12961 taataggtgt ataataaaag catgggaata gatatttctg ccaagaagtc gaagttccct
    13021 tctacgcaag gagagattgc ggcaagtgaa aagatcagcc acatcataga cttttcctca
    13081 aaatatctac acggaaaaga tacccccttg cggctttcgc ttctcacttt tttttcaaag
    13141 gggcacctcc tcattgagga cctgcccgga cttggaaaga cgacccttgc cattgccatt
    13201 gcaaagattc tgggtctcag tttcgggagg atccagtgta cgagcgattt gttgcctaca
    13261 gatattaccg gcctctccat ttacaataag aacacagggg agttcgactt ccatcccgga
    13321 ccgatattta ataacattgt cctggttgac gagatcaaca gggcgacgcc gaagacccaa
    13381 agcgcgcttc tcgaggccat gggggagaag cagaccaccc ttgagggaaa aacgtacaaa
    13441 cttccgaatc ctttttttgt gatcgcgaca cagaaccccg cggaacaatt cgggaccttt
    13501 ccccttccgg aatcccagct ggacaggttc atgctgaaga taggtatcgg ctatcctccc
    13561 aggggagcgg agaggaagat tctcaaggtg gggagcagca gagaagagct ctatgccata
    13621 gaacccatga tgacaaagga tgaggtcatc catattcagg aggagatcgg gcagaaggtg
    13681 gtaatctctg acaggattct ggaatatatt ctggatgttg tggaagcaac aaggaccagc
    13741 acctatttcc tggctggggt ctccacgaga ggggtcctgg cgctcaccac agtggcaaag
    13801 acgaatgcct attttcatgg aagggatttt gtcatccctg aagatgtcaa agaggttacg
    13861 gaatatacct taccccaccg ggtgatcttt aaggaagaat acgagaacct tgatcgaggg
    13921 gagattatac ggtcgattat cgagcacata cgggtccccg cgtaattacc ataacaaagg
    13981 ctggctggct ctatattacc gttactattc tcctcggctt ttctgccgta aataccggga
    14041 ataatctcat ctatctcatc gttgccgcgt ttctcagttt tatgggaatc tccggatttt
    14101 ttggaaggag aaacctcctg gacatccgtg tcagtgtggg acttcctgat gaaatctatg
    14161 cccgcacaag cgtgccgctg aaggtgacgt tgcacaacaa caggaggttc cttcccgcgt
    14221 ttcttataaa ggtacatctc cacgatcaaa gggtgctttt cccttttgtg gacgcaaagg
    14281 ccgagggcgt cacgtatctc gttaccacct tcaatgaaag ggggatcgtg aggatcaatg
    14341 actttcacct atgctccgta tttcccttta acttttttat tcgctgcagg aagatagaga
    14401 gggctattga ggtgaccgtg tttccccacg caaaaaggtg tgaactcccc ggtctttctg
    14461 agagagaaag gcagttgcgg ggcgaaagga gcatagacaa agcaggatat gaagccgaga
    14521 tcatgactat gagagattac ataaaagggg atcccataaa atatattcac tggaaggcaa
    14581 cggccaaaac aggaaaactc aagacaaagg agctctcaac cccttcccat cagcccgtca
    14641 taatagacat ggatgttgtc cccataaaaa atatggaaga aaggatttca tgcatcacct
    14701 atatcattct taagctcttc agagagaata tccctgtagg actcaaaata aagggtacct
    14761 tctataagcc ctttaaggtg gcgcagggag acggattaaa agtaaataaa atcaatatgt
    14821 taagggctct ggcggtctat gataaagagt agctcccccc tgaggttaga cggtgtggtg
    14881 aaggtattga cctatctcat agttgccatt ggggcttctt cggtcgtgag gtatatcggc
    14941 ctcttttatc cactggcatt tctttgcatc tttctcctct ccctgtacct tgattacagg
    15001 agaaggtttt ttgtccctcg atggctcctc acgggtgcgg ccctatcggt gatcgtgata
    15061 accctgttcc gggcccgtac cgatgatctg gtggacgcgt cccttggagc actcctcatt
    15121 ttactcggaa taaaattgtt aacggacaag agattcagag actacatgca gatatacctc
    15181 atcgtgctgt tcctcctcac cggttcctca ttattatccc tggacatcga attcttgttc
    15241 tattttatcg ccctgatctt tctggtaagc atagccatgg tgttattgac atactgctcc
    15301 caggacagtg cccttgagat gcctgtttcg agcgtgcgaa cgatagtgtc gaaatcgtcc
    15361 ctgatcccct tattcgcaat tcccatgact ctcttcatgt tcatcattct tccccgcacg
    15421 agctatccgc tgttctattc cttacagagg ggatcaagcg cacataccgg gttcagcgat
    15481 accgtgagac tgggcaatgt ttcggacatc caggaagacg ctgcggtggt tctcagggcc
    15541 cacatggaaa ggattgatga aaactcattg tactggaggg ggattgtcct tgataccttt
    15601 gacggcattt cctggcaaag catgcataag gaacttcctg acaaaaacag acctcccctg
    15661 ctttcaggga agcggatccc gcagactatc taccttgaac cttacgggaa caaatatctc
    15721 tttgcgctgg ataaaccttc ctccatttct ctgagatacg tgagcgggta tcgtgatctc
    15781 acctactctt ttcccttcat tctctccaaa atgatacgat acgaaacgtt atctgcactt
    15841 tctgacgtgt ttgttgaccc agaaatcgac aggagcgtgt atctccagct gcctggagca
    15901 agactcgcgc gcacaaagga gttggcccgg tccctttccc gggggaccca ggaagaaacg
    15961 atacaggaga tgttgaattt cttaaggaat ggtacttacc tctattcctt agaaaacctc
    16021 cccctttccg gcaacccttt ggaggatttt ttgttcaaca acaaatacgg gaattgtgaa
    16081 tactttgcgt cagcaatggc ggtgatgctg aggatggcgg gaataccctc cagggtcgtg
    16141 ggagggtaca ggggagggta ctacaatgat accggagaat attaccttgt cccccagaag
    16201 aacgcgcatg tatgggtcga ggcatacctt gagagcggga ggtggaaacg attagatccc
    16261 acgccggcag ggatagaaca tttcgtttcc ttcaccagag agaacctttt ttttaaggta
    16321 agattttttc tggacacgat taactactat tggaatgccc tggtcattaa ctatgacttt
    16381 gcaaaacaaa tgaccctcct ccaaaaactg agatcattca agaagccaaa attcagtttt
    16441 ccggtaagca aggaacagct gctcagatat tccgcagcac tcctggtctt tgggctttcc
    16501 gtttggttga tttatttctt gatttccaaa agaagggccc cggaggaaaa gctgcgggag
    16561 gcatttatca ggaaaatggg gaaatatgga tacagaaagg ccccatctga aggtcttgag
    16621 gaatttgtgt cgagagtaga ggaagcccct cttcaagaaa aggcgtaccg ctttgtgagg
    16681 gagtttgaac gcttttacta caaagatata ccgctcacca gggaagaggc gaaaagacta
    16741 aaacatctgc tctgacccat tttattccta agttctttca tgatgcgagc ctcttaagtt
    16801 tcactcttat caataatccg ggttaagacc tcgacccagc gcgcataccc tttctcactg
    16861 agatggactc cgtcagggag aaggtagtcc ttgattggat ttccctgagg atctacgaat
    16921 ctgttgtaca gatcgagaac ctctgtgtgc gtctcttgag cgattttgtg gagcaaacga
    16981 ttcatctccc tgatagtttc ttttggtatc catggcagca gagtgggcaa gagcgtgtgc
    17041 agaacgattc ttgtgcgcgg atatgcagag gacaacgctt caacgacctt tcgatatgaa
    17101 tcaagaaatc cgagatcttc catagcaaca ttgttgatcc ctgtcatgat gaggataagg
    17161 tctggtgagg ggaatttcct gattatcttc ccgatccttg aatagaggcc ctccaccgtc
    17221 tcaccggcaa tgccgaggtt tgcaatggta tggcctgaga atctctgctg ccaatcaaag
    17281 aactctacca gggaatcccc gatgaagagt atatgcttca tgtgtgtgct ttcaaaagaa
    17341 cgttgattac agattcttta gcaatgctat gaccgcctcc cggcagtttg ctgctgcaaa
    17401 gcggatatcc catttgtgtt tatctctatt gctgacaata ttactaatcc cacgtatctc
    17461 gatcacagga gtgtcataca tggcacagac atgcgctacc gaggctcctt ccatattctc
    17521 gcagagtgcg ccaaatcttt tttcaagctc cagggccttt ttcacagtgc cagtgcatgt
    17581 agagactgtg acaaaagtgc ctgattgtat cttgcattcc ccccgttttt cctttaagcc
    17641 cttcttaccc tgattttctg agggggggca aaagaaaaga ggagagagta tgtttttcgc
    17701 ccttctgata aggggcttat caagggggaa ctcattgaaa tagtttcgtc ttctttttct
    17761 caagaggggg atgcctatga actctgttcc atgaaactca tccttcagca taaccccctc
    17821 atcaccgtac acttctttct ctgctaccac aatatcacca atggtaagac cagcggacgg
    17881 gtatgctcct ccaatcccga aaaggacaag catttcgggt gagaacttct ccagcagaat
    17941 ggtcgccgca tgagcagcat ttgtcttccc catgcctgag gttatgtgga cgacagcttt
    18001 attgttgatc ctgcctttat acacaggctt accggcgaga agcgtttttc tctttagttg
    18061 tctggcgagc aaggcccctt cttcagggac tgaggaaata agagcgatca tgtccgtaaa
    18121 gggtggtcct tgatgagaca tgaagtgaat aaagaggtta acatggggca gatgggcgaa
    18181 gaagttttat acgacacctt ataaaaaaga cctcctggga gatttggagg aaaccacgct
    18241 gttcaagaga tgacaatccg tttacaaata tcctgtcgat tataaaattt ctgagaccgt
    18301 ctgcccatgt ctgtttaacg atcctgtttt tattccctca actgaccgct tccaaagacg
    18361 atgaactttg tgcacgtgag ctcttcaagt cccatgggcc ccctcgcatg tatcttgtcg
    18421 gtggatatcc cgatctccgc gcccagacca aattggtatc cgtcattgag ccgcgtggat
    18481 gcattcacaa aaacggctga ggaatcaacc tgcctcagga acctcatcgc cctgtcatag
    18541 ttcatggtga cgatggagtc agaatgggcg gaactgtatt ttgcgatatg ctccattgcg
    18601 tcatccatat ccttcaccac ctttacgttc aaaatcagat caagatactc cctgtagtag
    18661 tcctcctctt tcgcctgcgc gatgtgagga tagatcccca gggtcttcga gcaccctctg
    18721 agttcaaccc ctgcatcctt aaacctcttg agcatcttgg ggagaaaccc cgcggcaatg
    18781 aactcgtcta cgagcatcgt ctccatggca ttgcatgtgc cgggcctctg aacttttgca
    18841 ttaaagcaga tctcttctgc catgatcaga tcagcatccc tgtcaacaaa cacatggcat
    18901 acccccttgt aatgtttcag caccgggatg cgggaattct cggtcacggt ccggatgagt
    18961 ccctctccgc ccctgggtat gataaggtcg atgattcctt cgagcttcag catctccatg
    19021 attgcttctc tctcgggaat gtcgatgaag gtcaccgccc cttcgtgaat gccctgctcc
    19081 cttgccgtgt ccctgaggat cttcacaatg gccttgttgg aatggatcgc ttcagagcct
    19141 ccccggagga tcaccgcatt gccggtctta agacatagac ttgttgcgtc tgcggttacg
    19201 ttgggtctgg attcatagat aatcccgatc actcctatag gaacgcgcat ttttcccact
    19261 gccatgccat tgggtctttg ccacatcttt attacttctc ccaccgggtc agggagctgt
    19321 gaaacttcaa gaaggccctg ggccatctcc tgtatgcgtt tttcattgag ggtcagcctg
    19381 tcgattaagg cctttgaaag acctttcttc tccgcatacc cgatatcctt cttattctcg
    19441 gaaatgagtt ctggtgctct ctctttcagg gcttccgcca ttttgacgag ggcaaggttt
    19501 ttctgagacg acgaagcctt tgcgagggcg cgtgcgcctg cccttgcctc aaccgctaaa
    19561 ttatagacat actgctttat atccatagag atgcctcccg ggaaaatagc taattatcaa
    19621 actacaataa ccaaacgatt tgaaaataat caatatagat agctcgcggg agagcaaaac
    19681 cgaacaaggt ttccagttca cgccttgaat ctcaagtaaa tatagactcc gataagaaaa
    19741 ggctcagact tagtgactag gtttcccatt atgtgcctga ggaatagatt aaaagaaatg
    19801 tgcccacccg tctcatcaac tctacgggca accaactccc ttgccaattg tgcggcctcc
    19861 aatgaaaaga cggtgaggac atgaagagaa aagaatataa tcagcgcctc tatataagcc
    19921 ctgcgccttc gtcgaataaa cgggaatagc gcggccatta tgccaatggt tgtaggcgta
    19981 ttgaacgtat atgccgaggg gtgaatagaa acatcaatga gaatttctgc acttatcctc
    20041 agtggtctga atgttgcctg aatggtatct tttcctttcc aggatatccc ctcacagtta
    20101 agatccttaa acgcggtaac cagttcagag cctacataca tcaccgtata gtcatattgg
    20161 tctttaatct gtagccagag agccagcgac atgagataaa agcagacaaa gatgagaagc
    20221 gctttcagga ctgcggtctt attcctcaag cgtcttttga tttcctgcat aggtgagata
    20281 aataaaaaat acggcaaggg agattgctat cattaagccc tgtgttacgt agttatgaat
    20341 gtagtcaagg aagttcttat aatggatcgc ggaaaatacg agagccgtta ttctcagaat
    20401 gtttgccgcc tgtattacca gaaaacccgc gaggacacca ataaatcttt ttttccacgg
    20461 tgcagggaac gccaccaccg cagcggaata tatcattacc gcctcaagac cgttgcaccc
    20521 gaacctcaca tcaagggtac tgcccggcac actaattatt gagtccctgt aagtgcaaga
    20581 gatgccggat attcccaacg cttctgccgt aacaatgact actgcctttg aatagacgtt
    20641 atttacgtca atcctttctt tcaggggttt atacacgagc acaaaggtca acagtcccat
    20701 cagcataaaa tactttatga gaaaactcca gatgctgatt tctccagttt ttctctttct
    20761 ctcgcctgaa tcgcttttgt tgtcttttct tccctgtgcc atctaatatt atacctgact
    20821 aattaacctg catgaaacaa taaggcgggg aatttctccc cgccttacat tctgaactat
    20881 ggaatttgaa ctacgaacta aattcttttc cgcttcctca aacagaatat cgacccgagg
    20941 cctgcaagga ccataaagat tatcatgccc cattctgtca tcgtgggaat ggagaatgga
    21001 tcggtatcaa tcaacagcac cggcccatag aattcactgt tactgccaag aatcgtattg
    21061 tactctgtag aatagaaagt cccttcaggg tcttgcgcct gtgagtctac gatccgccac
    21121 ccattattgg cgaaccagcc cagggcaaag cccttcacgt cagatgtcac atcccagcgc
    21181 atcaaactat tgatagcgga aagacccgtt ggtgtcgact gctgatccgt aaaagtgcct
    21241 gcaacgcccg gttgcaggtt aaaggtgatg gtattgacat cccatgctcc ggttacacga
    21301 tcgatattat aagtgcgtga actgaaaaaa ctgggcggaa caaggacacg catgcggacc
    21361 ttggccgagt ggacattccc cgggagtata gggagagtga agcgcacgaa cgtcttctca
    21421 agatcattag aatcgttatt gacatcaagg gtgtacttga aaagaaggct ggagtttgaa
    21481 ttaaaatcag gagaatgatt tgttatgtag gaatcgtctg cgccgatgac aacgatctgg
    21541 gccacgtcga aatataaagt gtaccgaggg ttggatccag tgaatgttgc gcttgagcat
    21601 gcattattat tatcaggcct ccagagttcg gccctccata tcccgctcgg acccgaagca
    21661 ggaacaacaa aagaatcatc acgggaggat gatgtcgagg catcgaggtt gtctgtcgca
    21721 actacgttgt tattcgggtc tatccacttg acttgccagc acggactcga gtttaagcca
    21781 ctggcccgcg catacaccgt tgaaccccag gcaaaggcat cgcgttgtat gttgtgtgtg
    21841 gcatctttat aagaacgcaa aagcggcgcg tcatcaaaaa tcgtctgagc cagaccggaa
    21901 gtatttcccg aggccaccag aaggaacgtc ttgctgtcat cattcacttg tattgaat
//
LOCUS       DATKOU010000047         4371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2367063, whole genome shotgun sequence.
ACCESSION   DATKOU010000047 DATKOU010000000
VERSION     DATKOU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367063"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..850)
                     /locus_tag="VMU21_02165"
     CDS             complement(386..850)
                     /locus_tag="VMU21_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267470938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HUO76356.1"
                     /translation="MPVYKTIFSLLFILSIASCARTTAVAPVNTTTEGVAIKGYDPVA
                     YFTQNKPVQGTTEFEYLWNGAKWRFAGAEHRDLFRTDPEKYAPRYGGYCAYAVSQGKT
                     ADIDPDAWTVFEGRLYLNLNKEVQGLWEKNMRGYIHKADENWPQMLGKRHGP"
     gene            1160..1612
                     /locus_tag="VMU21_02170"
     CDS             1160..1612
                     /locus_tag="VMU21_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76357.1"
                     /translation="MEAESFSDIELSGFINETFVPLEIHIKENPTGFHRFDVLWTPSV
                     LIMDAQGKERVRNEGYLPKAEFRAWLDMGLARLAFVAKKWAEAEARFDGIVKCNPESA
                     VAAYSVYWRGASRYKQSHDHKALSAVAEEFRHKYQESIWAKKSVVWGE"
     gene            1951..2202
                     /locus_tag="VMU21_02175"
     CDS             1951..2202
                     /locus_tag="VMU21_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76358.1"
                     /translation="MSFSETANSILSFSKDNPIIAIVIGLILLFLLFRKPKLFFGLLF
                     LGAILALVWYFIADVSSKGKSGKEEMIEKGINPPMDNIR"
     gene            complement(2316..2789)
                     /locus_tag="VMU21_02180"
     CDS             complement(2316..2789)
                     /locus_tag="VMU21_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76359.1"
                     /translation="MRRLSLGTSAALFLVFAFLCTGTIVAGQDKSCTPTEPDMLGPFY
                     KSDAPVRSSVGKGYVLSGVVKSSAGCVPIKGARIEFWLAGSNGKYDDEHRATIFSDEK
                     GFYQFESNIPEPYFGRPPHIHIKVSAKGFTTLITQHYPEKGKAGASFDLVLVPSR"
     gene            complement(2880..3770)
                     /locus_tag="VMU21_02185"
     CDS             complement(2880..3770)
                     /locus_tag="VMU21_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUO76360.1"
                     /translation="MKQTRKARGSFSLGSFLLQVLRGFRRNQGLLLAGAVAYYTLLSL
                     MPMLILTLVGLSHVIEEEELFQTVSAHLDMVIPGYASTLTEQVRTFLAHRHVVGTVGF
                     IALLFFSSTAFTVLENAMSVIFYHRVRIKRRHFLVSAVIPYVFIGLLGLGVMLVSFIA
                     GALQTMEKNNLVIFGSRVDLRGASVAVLYVMGLLGEVFMLTSLYMVMPVGGITFRHAL
                     LGGVTATVLWEITRRVLVWYYSTLSMVNIIYGSLGTAIVLLLTIEVAAIILLLGAQVI
                     AEFERSRGILAHDKGSGFQT"
     gene            4170..>4371
                     /locus_tag="VMU21_02190"
     CDS             4170..>4371
                     /locus_tag="VMU21_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76361.1"
                     /translation="MVRKKKSAEESAEFIDSLKEWLKLEDETIKHANSTMKKTKNKLV
                     WMTMEMIKHDSEKHKAMQKMLID"
BASE COUNT         1200 a         1035 c         1101 g         1035 t
ORIGIN      
        1 actcaaagag gggaagaaga agattaatct taagtatcct gaggttattg gtatcaaatc
       61 ccttgaggtt gctgtttgag cggacaggga ctcccgatac tttcttcaag agaaataatc
      121 aagatcctcg aaaggattgg atttcaaaat acaagaaaat caaaaggcag ccatttcaga
      181 tatgcccacc ccgatggaag gaaaacaact atccctgtcc ataaaggcag aacaatcagc
      241 cgaggtctcc taagaaaagt aatccgtgat atagaattgt ctacggaaga attcttaaag
      301 ctcactaaga agcaatagac tgcctaaaat caatcgtgaa acatgaagag tgattcgtga
      361 atagtaacgc gtttctgtct ccctgttatg gaccgtgtct cttcccgagc atttgcggcc
      421 agttttcgtc tgccttatgg atatatcccc tcatgttctt ttcccacaac ccctggactt
      481 ctttgttaag gttcagatag agccttccct caaagacggt ccatgcatcc gggtctatgt
      541 cagccgtttt cccctggctc actgcatagg cgcagtatcc tccatatctc ggggcgtatt
      601 tttcagggtc tgtcctgaag aggtcgcggt gctcagcacc cgcaaaacgc catttcgccc
      661 cattccagag atattcgaat tctgtggtgc cctgtacagg cttgttttgg gtgaagtaag
      721 caacagggtc gtagcctttt attgccacgc cctcagtagt ggtgttgaca ggggcaactg
      781 cggttgtcct ggcgcatgaa gcaatgctca ggataaacag cagggaaaag attgtcttat
      841 aaacaggcat agagaaattg tagcacagac cagggataca tgctatctgc cttagcagac
      901 ccaaccaatg tgaatcctac agggctgatg atgctcttac ccgagggctt gggaacgcag
      961 gggagaagtt gcgaaggctg gggtcatggg atacaatgaa aagaaagtca cctggggata
     1021 tcacgattcg cgggaggtat aagacatggc tgtgaaatgg agaaaagatg tcgatgctgc
     1081 ccttgccgag gcgatgaaag agaaccggcc gctcatgatt gatttcagcg ctgctccgga
     1141 ctgaagcggc tgtgctcggc tggaggccga atcattttct gacattgagc tgtccggttt
     1201 tattaacgaa acctttgtgc ctctggagat acacatcaag gagaacccca caggcttcca
     1261 tcgttttgat gtgctctgga ccccgtcagt gctcattatg gatgctcaag gcaaggagag
     1321 ggtgcggaat gagggttatc tgccgaaggc tgaattccgg gcttggctcg atatgggtct
     1381 cgcaaggctc gcctttgtgg caaaaaaatg ggctgaggca gaagcaaggt tcgacgggat
     1441 cgtcaagtgt aatcccgagt cagcagtggc agcctattcg gtctactggc gcggggcgag
     1501 ccgttacaaa cagtcgcatg accataaggc cctctctgct gttgctgagg agtttcggca
     1561 caaatatcag gagagcatct gggccaagaa gtctgtggtc tggggggaat gaagagcaac
     1621 acttcttctt ccgttcaggc actgcgaaaa gcaggcggac ccggccttta catatttgtc
     1681 aggcactaat cgcttgcctc ccctgttgaa caatgtagtt gccctttttg caaccctttc
     1741 cttatcaaat caagaaagtc ttatgcccaa atcaatcgaa agttcaatag cgcacacgtg
     1801 tactatgatc cgcagttcca ttgcgccaaa agactagcgc ttttctattt cagcagccgt
     1861 agttttgagc gccttgcact ggggagagtt aatggtcaaa aatagttaca aatatgatat
     1921 aattttttaa caaaaacgaa aggggggtct gtgagcttct ctgaaacagc aaatagcatc
     1981 ctttcttttt ccaaagacaa tcccataatt gctatagtta ttggacttat tcttcttttc
     2041 ctgctctttc ggaaacctaa actctttttt ggccttctct tccttggcgc gatattagcc
     2101 ttagtatggt attttatcgc ggatgtctcc tcaaagggaa aatcagggaa agaggaaatg
     2161 atcgaaaaag gaattaatcc cccgatggat aatatacgct gatttattcg atcctcaaag
     2221 aacaattgag cggggaaatt acttagcctc ttttttcacc tgtcaaaagc agccgcaata
     2281 gtcacccttc ttgccgggaa tgcaaaaaag acttgctatc gcgagggaac cagcaccaga
     2341 tcgaaggacg ctccggcttt tcccttttca gggtaatgtt gcgtaatgag tgtggtgaag
     2401 ccttttgcag agaccttgat atggatgtgc gggggacggc cgaagtaagg ttccggaatg
     2461 tttgactcga actgatagaa acctttctcg tccgaaaaaa tggtggcgcg atgctcatcg
     2521 tcgtacttcc catttgaacc ggcaagccag aactcgatgc gcgccccctt tatgggtacg
     2581 caacctgcag aggacttcac tactccacta agaacatatc ccttcccaac gctggaacgg
     2641 acaggcgcat ccgatttgta gaagggaccc agcatgtcgg gctcagtggg tgtgcaagac
     2701 ttgtcctgcc ctgccacaat tgtgccggta cacaaaaaag caaagaccaa aaagagggct
     2761 gcagaagttc cgaggctcaa ccttctcata tcttaacttt accgcttttc ccctgtcttt
     2821 gcaatctcat cacctatacc tggaagggtg taaaggccgg caatgaaaag atgctgaggt
     2881 tatgtctgga accccgaacc cttgtcatga gcaagaatcc cccttgaccg ctcgaactcg
     2941 gcgattacct gggccccaag cagaaggatg atggcagcaa cttcgatggt gagaagcaaa
     3001 acaatggcag ttccaagcga gccgtagatg atgttcacca tggagagggt ggagtagtac
     3061 cagacaagaa cccgtcgggt gatctcccac aggaccgttg cggtgacccc gcccaggagg
     3121 gcatggcgga aggtgatgcc tcctaccggc atcaccatgt aaagggaggt gagcatgaat
     3181 acctctccga gaagacccat cacatacagc actgcaacag aagcgcccct gagatctacc
     3241 ctgctgccga aaataacgag gttgttcttc tccatggtct ggagtgcacc cgcgataaag
     3301 gacaccagca tgacgccaag gccaaggagt ccgatgaaga cataggggat gaccgcagag
     3361 acaaggaagt gtcgccgctt tatccgtaca cggtggtaaa agattactga catggcgttt
     3421 tcaaggaccg taaaggcggt cgaactaaaa aaaaggagcg ctatgaatcc aactgtgcct
     3481 accacatggc ggtgagcgag aaatgtcctc acctgctcag tgagggttga cgcataaccc
     3541 gggatgacca tatcgagatg ggcagacacg gtctggaaga gttcctcctc ctcgatcacg
     3601 tgggagagcc ctacaagggt gagaatcagc atgggcatca gggagaggag ggtgtagtac
     3661 gccacggcgc cggccaaaag gagaccctgg ttccgcctga aaccgcgcag tacctggaga
     3721 agaaaactgc caagggaaaa ggagcccctg gcttttctgg tctgcttcat ggggccgcgt
     3781 gacttttcca tattaaactc tacccttttt ccccgctctt ggcaatctta ttttctccct
     3841 caggagacgg acatttacga ttctcaccct cctcactcag ttctcctctg tggccacagg
     3901 ataaaaccag ggctatactc cgattgcccc tctggatgga agtctttatt tttcatcctt
     3961 ctctcttttc agctcgatga ccaaccattc aggcaacacg aatgacgatg gactgataac
     4021 atttatttat ccacaaattt aataaattaa gcaggagaat gtaagcatga tacggggaat
     4081 cgtggtaagc tattcatcaa aacgaagaga ggccggtcct ttttgttgaa gcgggacaaa
     4141 tcatttaata aaagaaagga gaacagacaa tggtacggaa gaagaaatca gcagaggagt
     4201 cagcagaatt tattgatagc ctgaaagagt ggctgaagct tgaagatgag acgatcaagc
     4261 atgcgaactc cacaatgaag aagacaaaga acaaactcgt ctggatgaca atggagatga
     4321 tcaagcatga ctccgagaaa cacaaggcaa tgcagaagat gctgatcgac a
//
LOCUS       DATKOU010000048        17508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5063784, whole genome shotgun sequence.
ACCESSION   DATKOU010000048 DATKOU010000000
VERSION     DATKOU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5063784"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1234
                     /locus_tag="VMU21_02195"
     CDS             146..1234
                     /locus_tag="VMU21_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HUO76362.1"
                     /translation="MSKVKDDGSIWSGLAKKGVSRRDFIKYCTTVAATLALPSSLVPK
                     IVEALEKKQKPYAVWLEFSDCAGDSESMLRATKPTIGQIVLDVISLEYHETIMAGSGK
                     FAEKALQDVVKNQKGKYIAIVEGAIPMKDGGIYCTIGGKTAADIAKEVCGNAAMTLAV
                     GSCASWGGIASASPNPTGSVGVGKAVPGITYVNLPGCPMNVENVTATIVHYLTFGALP
                     ALDGEHRPLFAHGALIHNNCERRAHFDAGQFVKKWGDEGHRLGWCLYEMGCKGPEAYF
                     NCPTIRWNGGTSWPVKAGHPCFACASTNNWDAFGPIYKRLENVPGAGYQTSADKIGLG
                     VAAVAAAGVAAHAVIRATKGKPHEEEKK"
     gene            1256..1435
                     /locus_tag="VMU21_02200"
     CDS             1256..1435
                     /locus_tag="VMU21_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76363.1"
                     /translation="MAKIAIDPVTRIEGHLRIEAQVDAGKVTDAWSSGTMWRGIETIV
                     KGRDPRECWALTSRI"
     gene            1457..2965
                     /locus_tag="VMU21_02205"
                     /pseudo
     CDS             1457..2965
                     /locus_tag="VMU21_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
     gene            2974..3615
                     /gene="cybH"
                     /locus_tag="VMU21_02210"
     CDS             2974..3615
                     /gene="cybH"
                     /locus_tag="VMU21_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HUO76364.1"
                     /translation="MNKKLYVWEFPVRLTHWLNALSITALFVTGLYVGAPYFRAVNED
                     QLIMAQMRYIHFIAGYVFTVSVLIRIYWWFAGNKYARLNQFIFVNSERWKDTIDTALF
                     YVFLKKDIPHDPGHTGIAGLTYFCMFMLFLLQILTGFALYSQTHVGAFWTLMGGWLFT
                     IIGQGPVRLIHHLIMWAIGIFLVLHIYISWHNEVAEGNGLISSIFSGYKTVEK"
     gene            3623..4120
                     /locus_tag="VMU21_02215"
     CDS             3623..4120
                     /locus_tag="VMU21_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HUO76365.1"
                     /translation="MSIAVVGLGNILLRDEGVGVHVVNAIKGKYSFSPEVEIIDGGTL
                     GLDLLHLIGGREKLLLIDAVNFNKEPGYIGIVEDDHIPSTLFAKLSVHHIGLSDVLFA
                     AKLLDLTPAKVRLIGIQPESLEVGLDMTECINGKIEAVIDTVIGTLKEWNIECVLLSP
                     LKSSA"
     gene            4129..4329
                     /locus_tag="VMU21_02220"
     CDS             4129..4329
                     /locus_tag="VMU21_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HUO76366.1"
                     /translation="MATVDVYGARREISLMILPEEVGIGDFVIVHAGFAIQKIEREAG
                     NEALRLLSELVESDAFKEEYLE"
     gene            4326..4919
                     /locus_tag="VMU21_02225"
     CDS             4326..4919
                     /locus_tag="VMU21_02225"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HUO76367.1"
                     /translation="MKRYILAISGASGSVFGIRLLKHLIKRSEVHLIISSQSFSIISH
                     ETGIDWSAGTASQKEKKIRAHLRSRRVYYHNELNLASPLSSGSFITQGMLVVPCSMKT
                     LSGIAHGYANNLTERAADVTIKEGRPLLLSPREMPFSAIHLENMLKLARLGVTIAPPV
                     SAFYHKPGSLDDMVDFVAGKIMDSFGIRHTLFRRWGD"
     gene            5150..6529
                     /locus_tag="VMU21_02230"
     CDS             5150..6529
                     /locus_tag="VMU21_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUO76368.1"
                     /translation="MPLDLREIIERHQGEQLSLLSQYINPQMAKVLKTIGFDPVYVRG
                     KGSHLWDDRGNDYLDMISGFGVFALGRSHPKVKEAIRQYLDLDSPNLVKMGTQLLAGL
                     LAQKLVNEFAPPGLDTVFFCNSGAEAVDGALKFAHAFTGRKRFLYCDHGFHGLTLGTL
                     AISGCKEFRADYEELLSGGTEMPFGDIAQLERELSRGDVAALVVEPVIGHGVFIPPDD
                     FLPRARELCTRYGTLLIADEVQTGFGRTGKLFACEHWNVVPDIMTLSKSLSGGYCPIG
                     AILYPRKIYDKVFSSMDRCMVHSTTFSQNDLAAVCGLATLEVLREEHLVENSARMGTY
                     LVEQLRVLAQKYELIREVRGLGLMIAVEFGQPSSLSLKTGWKMIHQINKDLFCQSILV
                     PLIMDHHIIAQVSGHGRDIIKLIPPLVFNQNDADRFIEAFDQVLAAAHRFPGPIWEVG
                     SRIAKAVLR"
     gene            6555..8897
                     /locus_tag="VMU21_02235"
     CDS             6555..8897
                     /locus_tag="VMU21_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HUO76369.1"
                     /translation="MKQNPSQEQKQSEGHAAAVALRAIFRSRAAYAGLGVGVALFACV
                     MAWQGIDDVAEALRRAGWGLILITLLHLPPLWADAMGWRILIPKGEQPPRRTMFKARW
                     IGESINDLLPVLQMGGNVVKAWFLVDRGVKVDRAGASVVVDVTLVVLSQILFTLLGLI
                     LVVPVIGSGKPLLVVLTGAAILATLLTGFYIAQQRGFFSFIVRVSKRIFGGNEWSSVS
                     HGASTIDTEVVRLYAERKTLIASGCWHMLSWFLGIAEVWIALHLLGQPVDIRTALLFE
                     SLGQAIRTGAFAVPGALGIQEGGYVFVGSALGIDPGVALGLSLARRVRELLLGLPGLA
                     IWQAPALNGVLRRGLCHAYSQEGTFVPPSKESALSKGVERHCKVNRSPVVRAINAGGN
                     LLGKGWLAKLGEEGILDTARSRTRLEDFGNPPIFDPLRRLLKSADNEAQLNLMGRLAT
                     RHDMVRLLSNRLRMEEDRKKNPGIAEEEIRRPVIITGLPRTGTTLLHNLLTLDPANRV
                     PLTWETMYPSPPPDAATYSVDRRIALVDNQIRWFHRLVKGFNRIHPVGARLPEECLLI
                     FSHSFVSYQFETTHRLPSYQEWLESQDLVHAYEVHRRFLQHLQWRCPGERWVLKAPAH
                     MFDFGAMFRVYPDACVVMTHRDPIEVTASNASLTATLRSAFSDDVNPLEVGPECSKRW
                     ADAINRALLSRDCNCAPADHFLDLYYIELVADPVATVRKVYEHFDLPFPRELGQKITQ
                     FLGTNPKDRYGKHRYSLERFGLDIEEENMRYSAYRERFRL"
     gene            9027..9341
                     /locus_tag="VMU21_02240"
     CDS             9027..9341
                     /locus_tag="VMU21_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUO76370.1"
                     /translation="MKKRTTMAILGIMSIFYPIITGCASGTSDGAKLFTSERCIYCHT
                     FKGQGAKVGPELTDVTKRRSDDWIRDQIRNPKLHNPHPGMPGHEYLSSKEINAIIQFL
                     KS"
     gene            9592..10641
                     /locus_tag="VMU21_02245"
     CDS             9592..10641
                     /locus_tag="VMU21_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUO76371.1"
                     /translation="MKRALVLAGGGARGAYQVGMLQELVIHKEIDFQIIRGVSVGALN
                     AAFLSQAPVAGDSLENLKKKIEDLHALWTNDIKGNDSVYAERGGFAAILAGADSLYSL
                     DPLRGLINEHVMLDNLRNSGRDFKVGTVSLVSGRYKEWAPDEIDFIDKLVASASIPVV
                     FPFVDFKSAEDVLVDGGVRNISPLGSAFKTLPDEIYVLLTSRLVKSDGGFPESGVQEQ
                     NYGQWSDNWLGTKVNGLDVLKRTIDILTDEIYLDDIRGALKWNEVIRKIDALKRSPHL
                     HTLPPELRSDFSKLIETSNVVKKRHVPLYVIAPQEWYGAQNNSTEFLPALMAQAIEHG
                     RMVASDPSKWLLPVQ"
     gene            10787..11029
                     /locus_tag="VMU21_02250"
     CDS             10787..11029
                     /locus_tag="VMU21_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76372.1"
                     /translation="MLKEGCPGSKEIRNPYPEEVVCFWCETPNEIWSDESEIACKHCG
                     KTISREMGFTCLQWCPAARECVGAEKYDRLMQAVKK"
     gene            11279..11752
                     /locus_tag="VMU21_02255"
     CDS             11279..11752
                     /locus_tag="VMU21_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO76373.1"
                     /translation="MNDGRVRLRPLRISDIPFMHKQLRDKDILKAIGLTRPFTGSWFH
                     LWWRLRKTLIFRYAIEADSRLIGFIGLHRLMPGISAELTLLIADEKSRRLGHGARAFT
                     LLAQNLEKYSSTRKLIVRVRPDNEAACSFWEKMGFEMRYDEYGMHVMSAELKFPD"
     gene            complement(11796..13814)
                     /locus_tag="VMU21_02260"
     CDS             complement(11796..13814)
                     /locus_tag="VMU21_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HUO76374.1"
                     /translation="MGNNNFVKPGMSAREIVSAVRALRGVCFTSVGMRDAGQSDFKNR
                     HRIYDLRTLAPYYRDMGLFSAETHGGARWHVGIMNRRESPFEEVNILRELMPDVLQQT
                     LVRETNLFGYRPYPKNIIEYVVSRVDIDVWRCFSFLNDVRNMRTVAEVVMKRGKLFQP
                     SISFTAADWTTNEYYLGVVRDIVDLCGGVDEIILCIKDMAGVGDIKRIYRLVDAIKQE
                     YPELLIHYHRHMTDGLAMPALLSAAQAGAKSFDVQEDSLVRFYGHPPILGVQSYFEES
                     GIPVHLDRKEAEAAVQKVREWIGHYEWAESPFKGYDHTVTLHRMPGGAFPSSFEQADK
                     GEFLHLMPAILKMMSLYNRIVKYFDVTPGSQMTWVTCNGIINRYAKERGDAGVRHVID
                     LVSRFVEEKDQDFDAMETHEQEELLTLFSNAPGDLKNLLLGNYGRLPVGWPADWVYRS
                     VFADEWKAKIRERKELSPLELVKDDDIERMRRELTEHIGRTPAEEEFILYTMHPKDAI
                     EYIQFRDKFGEAPLVLPTDVWREGLRKPGDRVDFELWGKPYSLELVSIGAEHEGIIHV
                     VMRVNNKTTVYPVETPRARRVEIRMAKGANHIGAPINGSVWRVGNPERGVLKAGDIVH
                     RGEEIANLEAMKMENGILAPFDAQILEVCVKLNDTVREGQLLFVIEKV"
     gene            complement(13823..15283)
                     /locus_tag="VMU21_02265"
     CDS             complement(13823..15283)
                     /locus_tag="VMU21_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HUO76375.1"
                     /translation="MMEKKIKKILIANRGVPAVRIMHTCRDRKIPTTAVYSDPDRLAH
                     HVFMADTAVHIGEAPPAESYLNMDKMIHAALKSKSDAIHPGWGFLGENEIFAQKVIDA
                     GLTWIGPSPEVIRMMGDKIQAKVLAQKANVPTIPGISNVSSPEQISKWMKDEGVTFPI
                     IIKAAAGGGGRGMVKVETEGQMSQALSQARSEAKKSFGSETVLVEKYIERGRHIEVQV
                     VADQYGNVVHLFERECTIQRRNQKIIEEAPSPSLDEDLRNEICITATRLMREIGYTTA
                     GTVEFIFDSSTREFYFLEVNTRLQVEHGITELITGLDIVGIMIDVAMGKHLPFKQSDI
                     HPNRWALEVRLNAEDPKTFSPSFGTISRLQIPMGPNVRVASGVYEGADIPPYYDSLFM
                     LLMTAGADRKDAIMVMDRSLGRNLRVEGIRTLQPLLLSIIRHPSFIEGEFSTRFLEEH
                     MDELVSMFREKNSEDEVLKIARYVAEISALGPQKWM"
     gene            15537..15707
                     /gene="tatA"
                     /locus_tag="VMU21_02270"
     CDS             15537..15707
                     /gene="tatA"
                     /locus_tag="VMU21_02270"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUO76376.1"
                     /translation="MFGLGVPELMVILVIALVVFGPSKLPSLGKSLGEGIRGFKKAME
                     DNPHEEKKSVEK"
     gene            15715..17298
                     /locus_tag="VMU21_02275"
     CDS             15715..17298
                     /locus_tag="VMU21_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUO76377.1"
                     /translation="MTKFLLPVAAVVLFLSFFRLGSVRLFDVDEAVFSEATKEMVERS
                     DWITPTYNGENRYDKPILFYWLMAASYKVFGINEFSARFPSALSSFILCLALLFFVRH
                     FHGPQEAFYAAISFALSLYFVVYSHAAVTDMALALFIALSLMSFYLSVADERRSKTTS
                     WYAYGFYLFSALAFLTKGLIGILFPFGIAACFLLATRGIKGLGELLSLRGILLFLVVS
                     LPWYMAQLAINGQEFFQQFFIKHHFKRYTGVISGHRGPIYYYIPALAIGLLPWVAFLP
                     GGIRNVFKKSDQEEKNTGLPGVQDTLGLFALIWFAFIFVFFSFSTTKLPNYLLPAIPA
                     AFILIASGMTGLGTWKRYTHIAAAVLIAVMGSAFLIAPGYLSQFDIGPSDWTIAAALI
                     MYALVALFLYTAFSKKALYGAVAVFMLAYLSLLQVKALPLINDYLQGTLHKYSLYAKE
                     RLGSDERILTYGINNPSIVFYSGHRVVKVGNRDELLPLISQQKRLLVITKAKEAEGLK
                     TLGFGLIEEDGRYAILEKQ"
     gene            17285..>17508
                     /locus_tag="VMU21_02280"
     CDS             17285..>17508
                     /locus_tag="VMU21_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76378.1"
                     /translation="MKNNKWVLFVLLLPFFLFSFDQTAILWVRDLHTHNVDLSAALKY
                     IDRFINVIAHGSTLIGTAFLLYIIGRRYSQR"
BASE COUNT         4218 a         4534 c         4403 g         4353 t
ORIGIN      
        1 atgttctgat aacatggctt cagatgggag aaagagaggg gttcgtgggg gtcgcttttc
       61 taactttgac aaagacctat cattatatcc ataggaccct tgctctccat acaaaagaac
      121 ttttattaat ggaggtggat catctatgag taaggtcaaa gatgacggaa gtatctggtc
      181 aggcctcgcg aaaaaggggg tatcgaggag ggactttatc aagtattgca ccacggtggc
      241 tgcgaccctt gcgctgcctt cctcccttgt ccccaagatt gtggaggccc ttgagaaaaa
      301 gcagaaacct tatgctgtct ggcttgaatt ctcagattgc gcaggggatt ctgaatcaat
      361 gctcagggcc accaagccca ctatcggtca gatcgttctt gatgttatct ccctcgagta
      421 ccatgagacc atcatggcag gttcaggaaa gtttgccgag aaggcccttc aggatgtggt
      481 gaaaaaccag aaggggaaat acatcgcgat cgtcgaagga gccatcccga tgaaggatgg
      541 agggatttat tgtaccatcg gagggaaaac agccgctgat attgcgaaag aggtatgcgg
      601 aaatgcagcg atgacactcg ccgtcggatc atgtgcaagc tggggaggaa ttgcgagtgc
      661 cagtcctaat cccactggtt ccgtaggagt gggaaaggct gtaccaggga tcacctacgt
      721 caaccttccc ggatgtccca tgaacgtgga aaatgtgaca gccactatag tccattacct
      781 caccttcggg gcccttcccg cccttgacgg cgaacaccgt cccctctttg cccacggtgc
      841 cctcattcat aacaactgcg aaaggcgcgc ccatttcgac gcaggtcagt ttgtaaagaa
      901 atggggcgac gaaggccaca ggcttggctg gtgcctttat gagatgggat gcaaaggccc
      961 ggaagcatac tttaattgcc caaccatacg gtggaatgga gggacgagct ggccggtgaa
     1021 ggcaggacat ccctgctttg cctgtgcaag caccaataac tgggacgcgt tcggcccaat
     1081 ctacaaaagg cttgaaaatg taccaggtgc aggataccag accagcgctg acaagatcgg
     1141 ccttggtgtt gccgcagtgg cagcggccgg tgttgcagcc catgcggtga ttagggcaac
     1201 aaagggtaaa ccacacgaag aagagaaaaa ataaactatt aaaggaggag tgagcatggc
     1261 caagatagca attgatccag taacaaggat tgaaggacac ctgaggatcg aggcccaggt
     1321 tgacgccggc aaagtcaccg acgcctggtc gtccggcacc atgtggagag gcatcgagac
     1381 tatagtgaaa gggagggatc cgagggagtg ctgggcactc acctcgcgaa tatgaggggt
     1441 ctgaacaacg gtccacgcac agtgctccgt gagagccgtg gaaaatgcgt taggaatcac
     1501 tatcaccaat aatgcaagga taatcagaaa ccttattaac ggtattcagt atgttcagga
     1561 tcatgtgatc catttctacc acctccacgc cctggactgg gtggatgtgg tgagcgcctt
     1621 gaaggcagac ccagcaaaga cctccgccct cgcccagtcc atatcagact ggcctgtttc
     1681 gtccgctgca tatttcaaaa cagtccaggg taaggtgaag gcgttggtgg atagcggaca
     1741 gctgggtctc tttgccagcg gctattgggg acatcctgcc tataagctcc ctcctgaggc
     1801 aaatctcatg gcagttgccc actatctgga ggcccttgac tggcagaggg atgtcatcag
     1861 gatgcaggca atgcttggtg ccaaaaaccc ccatccccag acctggcttg tgggaggcat
     1921 ggcaatccct gttgacccca acagccagtt cgcattgaat gccgggagca tcgcccttat
     1981 gaatgagctt tcccaaaagg ccctcgcgtt tgtggaaaag gtgtatattc ctgacctcct
     2041 tgccgtggcc tctttctata aggattgggc agccatcggg gcgggtgtcg gaaattacat
     2101 gtcctatggc gaattccaga acgacaatgt gagcaacacc acaaactgct ggttacctgc
     2161 cgggtacatc aagaacaagg acctcagtaa agtattgccg gtcgatcaaa agaaagtcac
     2221 cgaagatgtg gcccacgcat ggtataaata cagtggcggc gatgacaagg cgttgcaccc
     2281 ttacgacgga gagaccaacc ccaactatac cggtccgaaa ccgccctatg aattcctcga
     2341 cacaaacaag aaatacacct gggtcaaagg gatacgttat gacgggcttt ccacagaagt
     2401 gggacctctt tccaggatgc tcatcgcata tgcatctggc cacaagagga taaaggaagt
     2461 ggtgggcatg gtgctccaga agctcggagt cgggcctgcg gccctgttct caaccctcgg
     2521 acgcactgct gcgaggggta tcgagacgct ggtgaccgct gagatgctgc ctgtctggat
     2581 agccgaactc gatgcaacga tcaagggggg agacctccgc atccataaca atgagaagtg
     2641 ggacccgtcc acatggccaa aggaggcaaa ggggtacggc ttccatgagg cgccccgggg
     2701 agctctcggc cactgggtgg tgatcaagga cggtaaggtt gcaaactacc agtgcgtggt
     2761 tcccggcggg tggaattcct caccaagaga tgccaagggg cagaggggac ctttcgaagc
     2821 ggcccttatc gatacgccca taaaggatcc caacaagccc ctggagatac tccggaccat
     2881 tcattccttc gacccctgta tggcctgcgc gatccatgtg gtggactctg actggagcga
     2941 gataataaaa gtgagggtag tataggagga gccatgaata agaaacttta tgtctgggag
     3001 tttccggtaa ggttgactca ctggctgaat gccctgagca tcacggccct ctttgtcacc
     3061 ggtctttacg tgggggcgcc ctacttccgc gccgtcaacg aggaccagct catcatggcc
     3121 cagatgcgct acatccactt tattgcgggg tacgtcttca cggtgagtgt gctgataagg
     3181 atctattggt ggtttgcagg gaacaagtat gccaggctga accagttcat ctttgtgaat
     3241 agcgagaggt ggaaggatac cattgacact gcgctgttct acgtgtttct caaaaaagac
     3301 atcccccatg atccggggca tacgggaatt gcaggactca cctatttctg catgttcatg
     3361 ctcttcctgc tccagatcct cacaggattc gcgctttact ctcaaacaca cgtaggcgcg
     3421 ttttggacgc tcatgggcgg ttggcttttc accataattg gtcaagggcc ggtacgcctg
     3481 attcaccatc tcatcatgtg ggcaatcggt atattccttg ttctccacat ctatatcagc
     3541 tggcacaacg aagttgcgga aggaaacggg ttgattagtt ccatattcag cggatataag
     3601 accgtagaga aatgaatcgc gcgtgtccat agcggttgta gggcttggaa atatcctttt
     3661 aagggacgag ggagtcgggg tccatgtagt caatgccata aagggaaagt attccttttc
     3721 tcctgaagtg gagataatag atggcgggac tctgggcctt gacctcctcc accttattgg
     3781 gggcagagaa aagctcctcc tcatcgatgc agtcaacttc aacaaggaac ccggttatat
     3841 cgggatagtt gaagacgacc atatcccttc taccctcttc gcaaaacttt ctgttcacca
     3901 cattggtctc tctgatgtcc ttttcgcggc aaaactgctt gatctcaccc ctgcaaaggt
     3961 gcgtctcatc ggcatacagc ctgaatcact cgaagtgggt cttgatatga cagagtgcat
     4021 caatggtaag atagaagcgg tcattgatac cgttatcgga acccttaagg agtggaatat
     4081 agaatgtgtc ttgctgtccc ctctaaaatc gtcagcataa atgatcttat ggccaccgtg
     4141 gatgtctacg gcgcaagaag agagataagt ctcatgattc ttcccgagga ggtgggcata
     4201 ggagattttg tcatcgtgca tgccggcttt gccatccaga agatcgagag ggaagcgggc
     4261 aatgaagccc tcaggctctt aagcgagctg gtagaatcag acgcgttcaa agaagaatac
     4321 ctggagtgaa acggtatatc ctcgccatat ccggcgccag cggttccgtt ttcggaatca
     4381 ggctcctgaa acatctcata aagcggtccg aggtacatct catcatctct tcccagtcct
     4441 tctccatcat ctcacacgag acagggattg attggtcagc cgggacagcc agccagaaag
     4501 aaaaaaagat ccgcgcgcat cttagatcaa gaagagttta ctaccataac gaacttaacc
     4561 tggcatcccc cctttccagc gggtccttta tcactcaagg catgctggtt gtcccctgct
     4621 ccatgaagac attatccggc atcgcccatg ggtatgccaa taatctcaca gaacgggctg
     4681 cagatgtcac cataaaggaa ggcaggccac tgctcctctc tcccagggag atgcccttca
     4741 gcgccataca cctcgaaaat atgctaaagc tcgcgcggct gggcgtgacc atagcgcccc
     4801 ctgtttcagc cttctaccac aagccaggga gccttgatga tatggtagat tttgttgcgg
     4861 gcaaaataat ggattctttc ggcatccggc acaccctctt caggagatgg ggcgattgat
     4921 taggaaggag ccattgtgac gccattcgcc gtagcaacac aaccatattc cggaaattct
     4981 taatgcatct taaccattaa caccccgggc aatacctcac tgccggtcat ccggaaatcc
     5041 acaggaaagc ttgtagccta gacctcggca gttgatttct tctttacgaa tatgatgaaa
     5101 tgaatctgca gaatcaacgc attgtgttat caatagtaag gagatttcca tgccattaga
     5161 cctcagggaa attattgagc gccatcaggg agagcagctc tcgcttcttt cccagtacat
     5221 aaacccgcag atggcaaagg ttctgaagac catcgggttc gaccctgtct atgtgcgggg
     5281 aaaagggtcc cacctctggg acgaccgggg caatgactac cttgacatga tctctggctt
     5341 cggggtcttc gcattgggcc gctcccaccc aaaggttaag gaggctatcc gccagtacct
     5401 cgatctggac agtcccaacc tggtaaagat ggggacacag ctgctcgccg ggcttcttgc
     5461 ccaaaaactg gtcaacgagt tcgccccccc gggtctcgac acggttttct tctgcaactc
     5521 aggggctgag gctgtggatg gcgccctcaa gttcgcgcat gccttcactg gaaggaaaag
     5581 attcctctac tgcgaccacg gattccacgg cttgacccta gggaccctcg cgatcagcgg
     5641 ctgcaaggag ttcagggcag actatgaaga gttactttcc ggtggcacag agatgccttt
     5701 tggagatatc gctcagttgg agagagaact ttcccggggc gatgtggcag cgctcgtggt
     5761 ggagcctgtc atcggccatg gtgtcttcat tcctccggat gacttcctac ccagggcccg
     5821 cgagctctgc acccgatacg gcacgctcct catcgctgat gaggtacaga ccggtttcgg
     5881 cagaacaggg aaacttttcg cttgcgagca ctggaacgta gtacctgaca tcatgaccct
     5941 ttcaaagtcg ctttcgggag ggtattgtcc aattggcgca atcctctatc caagaaaaat
     6001 ctatgacaag gtcttcagtt ccatggaccg ctgtatggtc cattccacaa cattcagcca
     6061 gaatgatttg gcggcagtat gcggtttggc gacactggaa gtgcttaggg aagaacacct
     6121 cgtggagaac agtgcccgta tggggaccta tctcgtcgaa cagttgaggg tccttgccca
     6181 aaagtatgaa ctcatcagag aagtgcgggg gctcggtctc atgattgccg tcgagttcgg
     6241 tcagcccagt tccctcagcc tgaaaaccgg ctggaaaatg attcaccaaa tcaacaagga
     6301 cctcttctgc cagtcgatac tggtacctct cataatggac catcatatca ttgcccaggt
     6361 gtcgggccac ggaagagata tcatcaagct gatccctccc ctcgtcttta atcagaacga
     6421 tgcggaccgg ttcatcgagg catttgacca ggtactagca gctgctcacc gtttccccgg
     6481 cccaatctgg gaggtaggct cccgtatcgc caaggctgtt ctgcgatagg gcagttagtc
     6541 aaggatatca gccaatgaaa cagaatccca gtcaggaaca gaaacagtca gaaggccacg
     6601 ctgcggcagt ggcccttcgt gctatttttc gctctcgtgc cgcatatgca ggccttggtg
     6661 tgggggtagc gctatttgcg tgcgtcatgg catggcaggg aattgatgac gtggcagagg
     6721 ccctcagacg ggcgggatgg ggattgatcc ttattactct cctccatctt cctccattat
     6781 gggctgatgc catgggctgg cgaattttga tcccaaaggg tgaacaacca ccaagacgta
     6841 ctatgttcaa agcccgctgg attggcgaat ccattaatga ccttctgcct gtcctccaga
     6901 tgggaggcaa cgtggtcaag gcatggttcc ttgtggatcg gggtgtcaaa gtggacaggg
     6961 ctggggcgag cgttgttgtg gatgttaccc ttgtcgtcct gagtcaaatt ctcttcaccc
     7021 tcttgggtct tatccttgtg gttcctgtta taggaagcgg caagcccctt cttgtggtac
     7081 tgacaggcgc tgccattttg gcgacccttc tcacaggatt ctacattgcc cagcagcggg
     7141 gatttttcag tttcattgtt cgtgtttcaa aaagaatctt tggcgggaat gaatggtcgt
     7201 cggtctcgca cggcgcttcc accatagaca ccgaggttgt acggctctat gctgagcgga
     7261 agaccctgat agcctcaggc tgctggcaca tgttgagctg gtttctgggc atcgccgagg
     7321 tctggattgc acttcatctc cttggtcagc ccgtggatat caggaccgcc ctgctttttg
     7381 aaagccttgg ccaggccata cgcacaggcg cttttgcagt gcctggtgcc cttggcatcc
     7441 aggagggcgg ttatgtcttc gtgggaagcg cactgggcat agatccagga gttgccctcg
     7501 gactttccct cgccagacgt gtcagggaac tgctcctcgg actgcctggt ttggccatct
     7561 ggcaggctcc tgccttaaat ggcgtattgc ggaggggtct ctgccatgca tattctcaag
     7621 aaggcacatt cgttccgcca tcaaaggaga gtgccctgag caaaggtgtt gaaagacact
     7681 gcaaggtgaa ccgttcccct gttgtgcgcg ccattaatgc gggaggtaat cttcttggta
     7741 aaggatggct ggcaaagctt ggtgaagaag gtatcctcga tactgcccgc tcccggacac
     7801 gcctcgaaga tttcggtaat cctcccattt tcgatcctct tcgccgcctg ctcaaatctg
     7861 cagataatga ggcacagctg aatcttatgg gccgtctcgc cacgaggcat gacatggtcc
     7921 gtctcctctc caaccgtctc aggatggagg aggaccgtaa gaaaaatcca gggattgcgg
     7981 aagaggagat acgcaggcca gtcatcatca ccggactgcc cagaacaggc accacgctgc
     8041 tccataacct ccttaccctt gaccctgcaa accgtgtccc cctgacctgg gagaccatgt
     8101 atccctcgcc gccacccgat gcagctacat actccgtaga tcgacgtata gcccttgtgg
     8161 acaaccagat ccgttggttc catcgtcttg tgaagggctt caaccgtatc catcctgtgg
     8221 gagcccggct tccagaggag tgcctgctca tattcagcca ctcctttgtc agctatcagt
     8281 tcgagacaac acaccgcctg ccctcctacc aggagtggct tgagagccag gatttggttc
     8341 atgcctacga ggtgcaccgc cgtttcctcc agcatcttca gtggcgttgc cctggggaac
     8401 gctgggtcct caaagcccct gcccatatgt tcgactttgg ggccatgttc cgggtatatc
     8461 ctgatgcctg tgttgtgatg acccatcgcg accccattga agttacggcg tccaatgcca
     8521 gcctcaccgc aactctcagg agcgctttca gcgatgatgt gaatcccctt gaagtcggtc
     8581 ctgaatgcag caagagatgg gccgacgcaa tcaacagggc actcctttca cgtgattgca
     8641 attgtgctcc agcagaccac ttcctggatc tgtattacat tgagctggta gctgatcctg
     8701 tagccacggt ccggaaggtc tatgagcatt ttgacctccc ctttcccagg gaactggggc
     8761 aaaagatcac ccagttcctt ggcacgaacc ctaaggaccg ctacgggaaa caccgttata
     8821 gcctggagag gttcggtctt gacatcgagg aagagaacat gagatactcc gcatacaggg
     8881 aacgtttccg gctctgagcc acagcgaaaa gtgatgttga caagtgcgct tttgtctgat
     8941 ataaggtaaa caatgcttct ttaatctctc tttcggtgca tggctgaaaa atttattaga
     9001 cgcagaaagg acccgggaaa tctgatatga aaaaaaggac aacaatggcc atactaggca
     9061 ttatgagtat tttttacccc atcatcactg gatgcgcttc aggcacctct gacggagcga
     9121 agctctttac cagcgaacgc tgcatatact gccacacctt caaggggcag ggggcaaagg
     9181 tgggacccga actcaccgat gttacgaaaa ggagaagtga tgactggatt agggaccaga
     9241 tcaggaaccc taaactccat aatcctcacc cagggatgcc gggccacgag tatctgtctt
     9301 caaaagaaat caacgccatt atacaattcc tgaagtcgta atgattttgc caataagaca
     9361 gcgccagcag atttgaccac ttcttcattg cttcaccttc agtttcagcc ttacaatggg
     9421 gagcctcctc cgccctttcc tctcttcgca gagtcacctg atgttcattt gtgcacactt
     9481 gcaatatatg cacaaaaaaa aacaagaaat gcatagaaat tctttctttc aactgggata
     9541 attttctatc aaaaagaact tatgtcaacc ttttccagtc tggagggaac tatgaaacgg
     9601 gccttggttt tggcaggagg tggagcacgg ggtgcatatc aggtggggat gttacaagaa
     9661 ctggtaatac acaaagagat cgattttcag ataataaggg gggtaagcgt aggggctctt
     9721 aatgcagcgt ttctctcaca ggcccctgtg gcgggagact cacttgaaaa tcttaaaaag
     9781 aaaatagagg accttcacgc cctttggacg aatgacatta aaggtaatga cagcgtatat
     9841 gcggaaagag gagggtttgc cgccatatta gcaggggccg acagtcttta ctctcttgat
     9901 cctctgcgtg gattaataaa cgaacatgta atgcttgaca atctcaggaa ttcaggcaga
     9961 gactttaaag tcggcactgt ctctcttgta tcgggacgct ataaagaatg ggcaccggat
    10021 gaaatagatt ttattgacaa actcgtcgca tccgcatcca tcccggttgt gtttcccttc
    10081 gtcgatttta aatctgcaga ggacgtgtta gtggatggag gcgtcagaaa tatttcacca
    10141 ttgggaagcg cctttaaaac actgcctgat gaaatttatg tgcttctcac aagcaggctt
    10201 gtaaagagcg acgggggctt tcctgaatcg ggcgtgcagg agcagaacta cggccagtgg
    10261 agcgacaact ggctcggaac aaaagtcaat ggacttgatg tccttaaaag aacaattgac
    10321 atcctcactg acgaaatata tcttgatgat atccgcggcg cattaaaatg gaatgaagtc
    10381 ataaggaaga ttgatgcact aaaaagatcg ccgcatctcc atacgcttcc cccggaactt
    10441 cgaagtgatt tctccaaact catagagaca tccaatgtag taaagaaacg acatgttcct
    10501 ctgtatgtca ttgctcctca ggagtggtat ggggcgcaga ataactcaac agaattttta
    10561 ccggcattaa tggctcaggc catagagcat gggaggatgg ttgcgtccga tccatccaaa
    10621 tggcttttgc cagtgcagta ggacagggta aaaaacatct tgagaataaa gagcgcgcat
    10681 ctttgttttt aaaatttgga aagccagggt tggatccata ttcatctaat acacccaata
    10741 tgatttatct catagcgccg tgaattcgtc atggtaaaat ataggtatgc ttaaggaagg
    10801 atgtccggga agtaaagaga tcagaaaccc ctatccggaa gaggtcgtat gcttctggtg
    10861 cgaaactccc aacgagatat ggagcgacga gtcagagatc gcctgtaagc attgtgggaa
    10921 gacgatctcc agggagatgg ggtttacctg cttgcagtgg tgtcctgcgg cgagggaatg
    10981 tgtgggtgcc gagaagtacg acaggctgat gcaggctgta aagaaatagc attagccttc
    11041 aaacacttct cgagatcgta accttcccct gttgcagccg tctcacatat ttttttataa
    11101 cgaaacagcc acattttaaa atcccctgat tctgtgatac catcttctta ccaggtactg
    11161 caaaatgatt tcatccctca aaactaaaac ctccgaaagt tctgccaaaa gacaccagga
    11221 aaggaataga tataatcttt ctcgacattc cgtgtcctgt ttaaaaatcc cgcgatatat
    11281 gaacgatggc agggtaaggc tgcgacctct gaggatttct gacattccgt tcatgcataa
    11341 gcaactgcgg gataaagaca ttttaaaagc cattggattg accagaccct ttaccggatc
    11401 atggtttcac ctctggtggc gcttaagaaa gacattgatt ttcaggtatg caatagaggc
    11461 tgactcaagg ctcataggct ttataggcct tcatcgcctg atgcctggaa tatctgccga
    11521 gcttaccttg ctcatagcgg atgaaaaaag cagacgcctc ggccatggtg ccagggcatt
    11581 caccctcctc gcacagaatc tcgaaaaata ttcttcaaca aggaagttaa ttgttagggt
    11641 gagacccgat aatgaggccg cgtgttcctt ttgggaaaaa atgggtttcg aaatgcggta
    11701 tgacgaatac ggcatgcatg taatgtctgc cgaattaaag ttcccagatt agcaactttt
    11761 ggcgttcaat tcaaatagca ttgctcactg acgcatcaga ctttttcgat tacgaagaga
    11821 agctggcctt cccggacagt atcattgagc ttgacgcaga cttcgaggat ctgggcgtca
    11881 aagggagcaa ggattccatt ttccatcttc attgcttcca gattcgcaat ttcctcccct
    11941 ctgtgaacaa tatcccctgc tttcaatact cccctctcgg gattccctac cctccataca
    12001 ctgccgttga tgggtgcgcc gatatgattc gcgcctttgg ccatcctgat ctccaccctt
    12061 ctcgcccttg gtgtctccac aggataaacc gtggtcttgt tgttcacccg catcactaca
    12121 tggattattc cttcgtgctc ggcacctatt gatacaagct ccagggaata gggtttgccc
    12181 cagagttcaa agtccaccct gtcgcccggt ttcctgaggc cctctctcca tacgtctgtg
    12241 ggcagcacaa gcggcgcttc accgaacttg tccctgaact gaatatactc aatggcatct
    12301 tttgggtgca tcgtatagag gatgaattcc tcctctgcag gggtcctgcc gatgtgttcg
    12361 gtgagttctc tcctcattct ctcgatgtcg tcatccttca cgagctccag gggagagagt
    12421 tctttccgct ccctgatctt cgctttccat tcatcagcaa acacacttct gtagacccag
    12481 tctgcaggcc agcccacagg aagcctcccg taatttccca gcaggaggtt cttgaggtct
    12541 ccgggagcat tggaaaagag cgtcagcagt tcttcctgct catgggtctc catggcgtcg
    12601 aaatcctggt ccttttcctc aacaaatctg ctgacaaggt ctatcacatg gcgtacaccg
    12661 gcatctcccc gctccttcgc atacctgttg ataattccgt tgcaggtgac ccatgtcatc
    12721 tgtgaccccg gggtaacatc gaagtacttc acaatccggt tatacagaga catcatcttc
    12781 aaaatggcgg gcatgagatg aaggaattct cccttatcag cctgctcaaa agaactggga
    12841 aatgccccgc caggcattct gtggagcgtc acggtatggt cgtatccttt aaatggcgac
    12901 tcggcccatt catagtggcc tatccattcc ctcaccttct gaaccgcagc ctcagcctct
    12961 ttcctgtcaa gatgcacggg tatccctgac tcctcaaagt atgactgtac gcccagaatt
    13021 ggcgggtgtc cgtagaagcg gaccagggaa tcctcctgaa catcaaagct ctttgcgcct
    13081 gcctgtgcag ccgacagaag ggcaggcatt gcgagaccgt ctgtcatatg ccggtgatag
    13141 tggataagca gttccgggta ttcctgtttg atcgcatcca cgaggcggta aattcgttta
    13201 atgtcaccca cccctgccat gtccttgatg cagaggataa tctcatccac ccctccgcag
    13261 agatcaacaa tatcgcgcac tacccccaga tagtattcgt tggttgtcca atccgcagca
    13321 gtaaaggaga tggagggctg aaagagtttt cctcttttca tcactacttc ggctactgtg
    13381 cgcatatttc tcacgtcatt cagaaaggaa aaacatctcc agacatcgat gtccactctg
    13441 gagacaacat actcgatgat gttcttgggg taaggcctgt atccgaagag atttgtttcc
    13501 ctcacgagcg tctgctggag cacatcgggc ataagctccc tcaggatatt cacctcttca
    13561 aaggggctct ccctcctgtt catgatgccg acatgccacc gggcgccgcc gtgggtttca
    13621 gcgctgaaca agcccatatc cctgtaatat ggcgcaaggg ttctgaggtc gtagattcga
    13681 tggcggttct tgaaatcgga ctggcccgcg tctctcatgc ccacagacgt aaagcagact
    13741 ccccgaagcg cgcgtaccgc actcacaatc tctctggcag acatgccggg tttcacgaaa
    13801 ttgttgtttc ccatgctctc tttcacatcc atttctgggg gccgagtgcg gatatctccg
    13861 ccacataacg ggcgattttc agcacctcat cttcactgtt cttctccctg aacatggata
    13921 caagctcatc catatgctct tcgagaaagc gggtggagaa ttctccttca ataaaagatg
    13981 ggtgcctgat gatactcagg agcagcggct ggagggtcct tatcccttcg accctgaggt
    14041 ttctcccaag ggagcggtcc atgaccatga tagcatcttt tctgtcagcc ccggcagtca
    14101 tcaacaacat gaacaatgaa tcatagtacg gagggatatc cgccccctcg tacacacctg
    14161 atgcaaccct cacattgggt cccatgggaa tctggagccg ggagattgtt ccgaaggaag
    14221 gactgaaggt cttcgggtct tctgcattga gacggacctc cagggcccac ctgttcgggt
    14281 gaatgtcgga ctgtttaaag ggcagatgct tccccatggc gacatcgatc atgatgccga
    14341 cgatatccaa cccagtgata agctcggtaa ttccgtgctc cacctgaagc cgggtgttca
    14401 cctcaaggaa gtaaaattcc ctggtgctgg aatcaaaaat gaattccacg gtacccgcgg
    14461 tggtatagcc aatctctctc atcaaccggg tcgcggtaat gcatatctcg ttacggaggt
    14521 cttcatcaag ggaaggggac ggcgcttctt ctattatctt ctgattgcgc ctctggattg
    14581 tgcattcgcg ttcgaacaga tgcactacat tgccgtactg gtccgcaacg acctgtacct
    14641 caatatgcct gccccgttct atgtatttct caaccagtac cgtctcactc ccaaacgatt
    14701 tttttgcttc agatcgtgcc tggctaaggg cctgggacat ctgcccttcg gtttccacct
    14761 ttaccattcc cctgccgccg ccccctgctg cggccttgat gataatgggg aatgtgaccc
    14821 cttcatcctt catccatttg ctgatttgct ccggactgct cacattgctg atgcccggta
    14881 tagtggggac attcgctttt tgcgcaagga cctttgcctg tatcttgtcc cccatcatcc
    14941 tgatgacttc aggggaaggg cctatccatg tcagtcccgc gtctatcacc ttctgggcaa
    15001 atatctcatt ctcgccaaga aaaccccatc ccgggtggat cgcgtctgat ttgcttttga
    15061 gtgcggcatg gatcatcttg tccatgttaa ggtaggattc tgcgggcggc gcctctccga
    15121 tgtgcaccgc ggtatctgcc atgaacacat gatgtgcaag ccggtcaggg tcgctgtata
    15181 ccgcagtggt ggggatcttc ctgtccctgc aggtatgcat aatcctcacc gcagggaccc
    15241 cccggtttgc aatcagtatt ttcttaatct ttttttccat catgggaatt gttctctctg
    15301 ccgccttgaa ggtttatata ctaccatgtc tcagggaatc aattccatac gcaataactg
    15361 ctcagtccag ggattgcaga gatgcctgaa gacggattgc tttagccgcg cctccccatt
    15421 ctgataaagc acggtccgga tgcattttac gtaaccataa agatttctta aggtgtataa
    15481 taaagaagct gggtagaaga tactcagcga ataggataac gaaaggagag agagaaatgt
    15541 ttggtttagg cgtgcctgaa ttgatggtta ttttggtgat cgccctcgtg gtttttggcc
    15601 cgagcaaact cccctcactg ggaaagagcc tgggggaagg gattcgcggg ttcaagaagg
    15661 cgatggaaga caatcctcat gaagagaaaa agagcgtaga aaaatagctc tctcatgaca
    15721 aagtttctcc ttcccgttgc cgctgtcgtt ctcttcctct cgtttttcag actcggctct
    15781 gtgaggctct ttgatgttga tgaagcggta ttctcagagg caactaagga gatggtggag
    15841 cggtctgact ggataacccc tacctacaat ggggaaaacc gctacgacaa gcctatcctc
    15901 ttttattggc tcatggcagc ctcatataaa gtttttggca ttaacgaatt ctccgcaagg
    15961 ttcccctccg cgctctcgag ttttatcctc tgccttgcgc tattattctt tgtgagacat
    16021 ttccatggtc ctcaagaagc cttctacgca gccatttcat ttgccttgtc cctgtatttc
    16081 gttgtgtatt cccacgctgc ggtaaccgac atggcattgg cgctcttcat cgccctctcc
    16141 ctcatgtcat tttatctctc cgtcgctgat gagcgaagat ctaaaaccac atcatggtat
    16201 gcctatggat tctatctctt ctcagccctt gcctttctca caaaggggtt gatagggata
    16261 ctctttccct ttggaatcgc cgcctgtttt ctcctcgcca caaggggaat aaaaggtctc
    16321 ggcgaactgc tctccttgag aggaatcctt ctctttctcg tggtctctct tccatggtat
    16381 atggcgcagc tggcaatcaa cgggcaggag ttctttcaac agttcttcat aaaacaccat
    16441 ttcaagagat atacgggagt gatatcaggc cacagggggc cgatatatta ctatattcct
    16501 gcattggcaa taggcttgtt gccgtgggtg gcctttttac ctggcggcat cagaaatgta
    16561 ttcaagaagt ctgaccagga agaaaagaac acagggctcc cgggcgtcca ggataccctc
    16621 ggactctttg cccttatttg gtttgccttt atctttgtat tcttttcatt ctcaaccact
    16681 aagcttccca attacctcct ccctgcaatt cctgccgctt ttattcttat tgcctcgggc
    16741 atgacagggc tcggcacatg gaagagatat acccatatcg ccgcagcggt gcttattgcc
    16801 gtcatgggtt ctgcgttcct gatcgctcca ggatatctct cacaatttga tatcggccct
    16861 tccgattgga ccattgctgc cgcgctgatc atgtatgcac tggtcgccct atttctctac
    16921 accgccttct caaaaaaggc gctctatggc gcagtggcag tcttcatgct cgcctatctc
    16981 tccctgctgc aggttaaagc attgcccctc ataaacgatt atctccaggg tacgctccat
    17041 aaatacagcc tctatgcaaa ggaaagactg ggcagcgatg aacgaatcct cacctatgga
    17101 ataaacaacc cgagcatcgt gttttattcc ggccacaggg tggtgaaggt gggaaacaga
    17161 gatgagctcc tgcctctcat cagtcagcag aaacgccttc ttgtcatcac aaaggcaaaa
    17221 gaggctgaag ggctgaaaac tttagggttt ggcctcattg aagaagatgg aaggtatgca
    17281 attcttgaaa aacaataagt gggtgctctt cgtattgctg ttgccctttt tcctcttctc
    17341 ctttgatcag actgcgattt tatgggtacg ggatcttcat acgcataacg ttgacctttc
    17401 tgcagcgctt aaatatattg accgtttcat taatgttatt gcgcatggtt ccacgcttat
    17461 tggaacagcc tttttgcttt atataatcgg acgccgatat agtcaacg
//
LOCUS       DATKOU010000049        10980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_450245, whole genome shotgun sequence.
ACCESSION   DATKOU010000049 DATKOU010000000
VERSION     DATKOU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450245"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..328
                     /locus_tag="VMU21_02285"
     CDS             149..328
                     /locus_tag="VMU21_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUO76379.1"
                     /translation="MSLMKSVMNFFSLREETQPVRNMGRNESCWCGSGRKYKNCHLHE
                     DEKKLKNYAANCVNS"
     gene            complement(377..1516)
                     /gene="comE"
                     /locus_tag="VMU21_02290"
     CDS             complement(377..1516)
                     /gene="comE"
                     /locus_tag="VMU21_02290"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:TIGR03846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit beta"
                     /protein_id="HUO76380.1"
                     /translation="MRSPEEELIAILKKAKVDFTSSLPCEKIKGLLRRVPEEFFHLPL
                     TREEEGVGISAGAALSGKRPAMFVQSSGVGNMINALLSLTGFYQLPLAIFVSWRGVYK
                     ERIEAQLPMGQNLPGILKGAGISHSPITTREDLEGSSKRLEAVYKKNTLHAFLLSPAL
                     WEGSAVRREKPEKRSHKREAGADYEGGIMQRISSPRLTRFEILNSIAPYLDSKVVVCN
                     LGIPSKELYFIKDQPSNFYMLGSMGMATPIGLGIALTSDKEVVVIDGDGSILMNPGTL
                     ATAASCEPKNLTILAIDNSSYGSTGDQPTLTGMCVDLEFLARGFGIKDTHTVSRKKEL
                     VDMLKRTHDGMRFIHVLAVPGNRDVPNIPLHHLAIKERVQGFLRR"
     gene            complement(1492..2748)
                     /locus_tag="VMU21_02295"
     CDS             complement(1492..2748)
                     /locus_tag="VMU21_02295"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR03844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HUO76381.1"
                     /translation="MSHFSLRCTECGRELKETFCAFCEHCRDALLVTEYQEKGFRDDA
                     GRGIWRFNWLPVHTPHFDQSDPVVYRSEGLSKHLGLENLYIAFNGYWPEREGSLATCT
                     FKEFEAAVVLQNAMENGVAGLTVASAGNTARAFAHLSALTGFPAIIVVPRMCLTEMWY
                     LEGAPKLPTLVVGDGDYADAIDVAKRVALTIGFPFEGGVKNIAKRDGLGVTLLEAVSV
                     MRQLPHHYFQAVGSGAGAIGVWEMAERFLKDGRFGTRLPVLHLAQNLPFAPMLRAWEK
                     DSRKLFPKDLRPELIGEITTRVLSTRYPAYSIRGGVYDGLRATGGRMYGIRNESVFGA
                     MDTFEKSEGIDIVPAAGVAVAALMEAVHAGVVQKSETVLLNVTGGGEKTLLRDKKTYE
                     VKPCFVSKKVTEREIEEQLCEVLKKN"
     gene            complement(2745..3950)
                     /locus_tag="VMU21_02300"
     CDS             complement(2745..3950)
                     /locus_tag="VMU21_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUO76382.1"
                     /translation="MRKGNTFTSHLTPMQLIELDACTQCGECLKFCPIQDVNSKPSIS
                     PPEKIRVFREFIKATEGLKARLFNPKDIDRKLLEDFTKAVFECTTCGDCGQHCSVGIF
                     TQRLWPMLRKEMVRRGFGPIGGQAKMPEVIKDTGNPYNMPAKDRFNPWFPEDVKVAEK
                     ADVAYYAGCTGAYSAQPMVKGDVLVLKAIGEPFTMLPPEEEVCCGFPLFASGQHGMLE
                     DLTKRLVQAYKAKGVKLLLSSCPCCVNIMSRDWPIFYGQELPFKIRHMTQFAAEALKS
                     GKLKIKEGLSERLIYHDPCYLSRGVGITEEPRIILRSISGVQLIEFDRYGVNSKCCGA
                     GGAARKVYPENANAIGRSTIDEAVRKKADRLIFSCPACYAQVNEGMQNHDKRIKITDI
                     MELLSGFIA"
     gene            4090..4923
                     /locus_tag="VMU21_02305"
     CDS             4090..4923
                     /locus_tag="VMU21_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HUO76383.1"
                     /translation="MIDIPAVNYPKPWLLFWAVVILSHGYMDYALYRKWISWNRGITQ
                     TATTSGRWGRALKIWAAEVFLQRQLFGLSSFRWLIHLLIFFGFLALAFLSLSTFFLKL
                     SHYLGVESSVTHYFLNGSGYIFIKVWGDSFGLALLLGLIIASVRRFLFRPAQQISGQM
                     DVFLLFFLLWLTLSGFMLEGLRLALVPVELARCSFIGCFFIPPGTYTLEELQPWLTLL
                     WVLHSFTGVGILVYLPHSKLLHSILSPLVIAMNAHEEQERKDLYWPDIAKHRATRLPR
                     D"
     gene            4875..6137
                     /locus_tag="VMU21_02310"
     CDS             4875..6137
                     /locus_tag="VMU21_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUO76384.1"
                     /translation="MARHSKTQGDKIAQGLTRRQLIELEACARCGECQVWCPVYAQDK
                     RECITARGKLQSLRRLVKETLPEDEKHEFLEGLYECSACGQCHIVCPVRINTPELWEQ
                     SRLSLANAGIPQPESQLKQLATIKEFNNSFGKPQQERGLWAKTAWEAGLLKSPVPLWR
                     DHPTSLIYFAGCTASFELAMQSVAVQTARLLQEAGVEFSVLGEDEPCCVSKLRRMGDP
                     DFSLEAKKRLNQFDRMGIATVVASCAGCFKGLHSDYSKLWPERTRVIHLTQFLDHLIR
                     ERNLRLVHEVPLVVTYHDPCHLGRHNRIYEEPRSILKAIPGLRLVEMSRHRAFSSCCG
                     MGGGLKVANSGIQHKMGAERVREAESTGAEAIVTPCQTCSLGLLNGVDEASSGMKVYH
                     LNEIIVRSVCPEVTSEEILSAFVRAAVR"
     gene            complement(6152..6838)
                     /locus_tag="VMU21_02315"
     CDS             complement(6152..6838)
                     /locus_tag="VMU21_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76385.1"
                     /translation="MGILLTLSTYAVLGIFFILLCGKLLTLWKAARTVRGYAPSPSFT
                     PRLVMKVAGDILFLSRLLNTNDLLWIGEWLFHCTFVLVVLRHLVFFLSPVPGWVAFLQ
                     PFGIFAGYLLPLSLIYILTVKLNREEGYFPSYNFFLLILLLLLSMTGILLRTVFKADL
                     VAVKGFVLGILGLSPQSVPLDFLFLMHFSLVLVVILYVPTHIFAAPLVMLDARQREEE
                     LRRLLHGEKR"
     gene            complement(6902..7723)
                     /locus_tag="VMU21_02320"
     CDS             complement(6902..7723)
                     /locus_tag="VMU21_02320"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO76386.1"
                     /translation="MEIVLELNVVDLHKSFSSPTEGGRIDAIREVSFTVPEGRFVCVI
                     GPSGCGKTTLLRMIAGLESPDRGKVLLGDKEITSPVREIGYIFQEPALLPWRTVIRNV
                     EFGLEVAGVPHEQRRQKAFAALKLVELQDFRDFYPKEISGGMKQKVALAMALVVEPRV
                     LLMDEPFVSLDCQTRNYMQVALLHLWEKTGKTVIFVTHNVEEAVFLGDEIICFTSRPA
                     EVGKVLRVDIPRPRDRTSTELNAIRKEILLFLAGEREKTRSINGMFTHVQDLSEI"
     gene            complement(7714..8460)
                     /locus_tag="VMU21_02325"
     CDS             complement(7714..8460)
                     /locus_tag="VMU21_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO76387.1"
                     /translation="MRYLPILCVVVIWQVLSFSGVIPGNRLPSPVNIILGFTELLTQG
                     LPPGYALAGHCIASLKRVLSGVAIAIVTALPLGIAMGYWTRARNYLTPFVEIIRPIPP
                     LAWVPIAILWFGIGDRSSSFIIFLGAFFPILLNTIGGVLSINRRLIESSIILGAKKRA
                     LFTKVLLPGAMPSIITGVRIALGIGWMTLVAAEFTGVKSGYGLGYMIMTARDIQRPDQ
                     IIAGMVVIGMIGYAMDFGIRQMEHKILKWR"
     gene            complement(8534..9490)
                     /locus_tag="VMU21_02330"
     CDS             complement(8534..9490)
                     /locus_tag="VMU21_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76388.1"
                     /translation="MKKVAVVASLVMVLLGALISMAASRDVRLGYLRNDLHHLAAWVA
                     MDKGFFREEGVNVEIAGIFNAGPEEMTAFASRSLDMGYLGVAPSATGLANRSARIRAV
                     ALANAEGSSVVVRKDSAIKEVRDLAGKTVAIPGYSSVQDFLLRKALEAAGADPKKVNI
                     IAIKPPEMIVALNAKQIDAFIAWEPHPSKAVTMGIGRVLISSPMIWKDHPCCVVAVDD
                     SFYKENPQVVKSFLKAHIRATAYIKKNPEDALRIGMKYTGMDEATVREAISHIQYHYF
                     AKEDDIKEYVRYLLKFGYVKSMNVDEFAREYLDLKAIKEVVQ"
     gene            complement(9554..9886)
                     /locus_tag="VMU21_02335"
     CDS             complement(9554..9886)
                     /locus_tag="VMU21_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76389.1"
                     /translation="MTKVLINPGACGMLVTVEVEKKEGKIFSVRITTECEMAEKLGKE
                     IPELTMMDAFKRLQENPVYKKGATCLRHVACPVPSGILKALEVEAGLNLPRDVSITFV
                     KTEKDSGT"
     gene            complement(9906..>10980)
                     /locus_tag="VMU21_02340"
     CDS             complement(9906..>10980)
                     /locus_tag="VMU21_02340"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HUO76390.1"
                     /translation="TAMHHIFLGLNPTYIGRSPFIPAVQRSLNIKARDLGLKINPAGY
                     IHVLPIEAGFVGADNVGVLIAQEQYFQDEMVLVIDIGTNGELLLGNKDRVCSTSCATG
                     PAFEGAQIKFGMRAAPGAIEKVKIDPVTREPQYKVIGKADWHTHIEGKINAKGICGSG
                     IIDVIAEMFKAGIIDKTGKFVMNLGTTRVRSGVDGKPEYVLAWAEETSINADITVTQG
                     DVRALQLAKGALYTGAKLMMQKMGVTKLDRVELAGAFGSHIDREASLTLGMFPDVPID
                     KVIVVGNAAGDGAIMALINKDKRREANERARWVQFVEIATEPAFEKEFMQAMHIPHMK
                     DKYPNLKAMLEEQKAPIASSIKG"
BASE COUNT         2485 a         3075 c         2676 g         2744 t
ORIGIN      
        1 gtttgtttga ttgggaaatc gacgctaaat aaaaagtgaa atcggcgtcg agtacacgaa
       61 agagccaacc ctcatgctcc ctggaaaaat gtgcgcccct tctgctataa tattatagtt
      121 tctaaacatc taatatgtga ggttgaatat gagtttgatg aaaagtgtta tgaatttctt
      181 ttctcttaga gaggagacgc agccggtccg gaacatggga aggaatgagt cctgctggtg
      241 cgggagcggt agaaaataca agaactgcca tctccacgag gatgaaaaga agttgaagaa
      301 ctacgcggca aactgcgtga atagctgaag cacccgctga gctgagttca gcccacaggc
      361 gtttcatcat aaaggattat cttcttagaa agccctgtac ccgctccttg attgcaaggt
      421 ggtgcagggg aatgttcggc acatccctgt ttccgggaac agccaggaca tgaatgaacc
      481 tcatgccgtc atgggtacgc ttcagcatat ccaccagctc ctttttcctg ctcactgtgt
      541 gcgtgtcttt tataccgaat ccccttgcca gaaattccag gtccacacac ataccggtga
      601 gcgtaggctg gtcacccgtt gatccgtaag agctgttgtc aatggcaaga atcgtaagat
      661 tttttggctc acaagatgcc gcagtggcaa gtgttccggg gttcataagg atgctcccgt
      721 cgccgtcaat caccacaacc tccttatccg aggtgagcgc aatgccaagc cctatgggcg
      781 ttgccatgcc catgctcccc agcatataaa aattggaggg ttggtctttt atgaaataca
      841 gttcctttga cgggatcccc agattacaaa ccacaacctt tgaatcaaga tacggcgcaa
      901 tactattcag tatctcaaaa cgggtaagtc ggggagaaga tatccgttgc ataatacccc
      961 cctcataatc agctcctgct tctcttttat gactcctctt ttccggcttc tcacgtctca
     1021 ctgctgagcc ctcccagagg gccgggctga ggaggaatgc gtggagggta ttcttcttat
     1081 acacagcctc aagcctttta ctgctccctt caagatcttc ccttgtggtg ataggggaat
     1141 gggatatacc cgcacccttc agaatacccg ggagattctg acccattggc aactgtgcct
     1201 cgatcctctc tttgtatacc cccctccagc tcacaaaaat tgcgagcggc agctggtaga
     1261 accctgtgag ggacagcagc gcgttgatca tgttcccgac acctgaactc tgcacaaaca
     1321 tagcgggtct tttccctgaa agggcagcac ctgcgctgat gcccacccct tcctcctccc
     1381 gcgtgagcgg cagatgaaag aactcttcag gtacgcgccg gagaagacct ttgatctttt
     1441 cgcagggaag ggacgacgtg aaatcaacct tcgccttctt cagaatcgcg atcagttctt
     1501 cttcaggact tcgcatagct gctcctctat ttcacgttca gtaaccttct ttgagacaaa
     1561 acagggcttc acctcatagg tcttcttgtc cctcaataac gtcttctccc cgccgccggt
     1621 gacatttaat aacacagttt cactcttttg cacaactcct gcgtgcactg cctccatgag
     1681 ggctgcaacc gctacgcccg ccgcaggcac gatatctatg ccttcgctct tttcaaaggt
     1741 atccattgcg ccaaagaccg actcattcct gatgccgtac attctccccc ctgttgctct
     1801 gagcccgtcg tagacaccgc ccctgatgga gtatgccgga tagcgggtgg agagcacccg
     1861 ggtggtgatc tcccctataa gttcaggcct caggtctttg ggaaaaagtt tcctgctgtc
     1921 tttctcccat gccctgagca tgggggcaaa agggaggttc tgcgcaagat gcaggaccgg
     1981 taaacgggtc ccgaatcttc cgtccttaag aaatctttcc gccatctccc acactcctat
     2041 ggcgcctgca ccgcttccta ctgcctggaa atagtgatgg gggagttgtc tcatgacaga
     2101 gaccgcctcc agcaatgtca cgccgagacc gtctctcttg gcaatattct tcacgccacc
     2161 ctcaaaaggg aacccaatgg tgagggcaac cctctttgcc acatcaatag cgtccgcgta
     2221 atcgccgtct cccacaacca gggtcggcag cttcggcgca ccttcgagat accacatttc
     2281 ggtaaggcac atcctcggca ccacgatgat cgcagggaag cctgtgagtg cagacaggtg
     2341 agcaaatgca cgtgcagtat tcccggcaga ggctacggtc aatccggcca caccattctc
     2401 catggcgttc tggagcacca ccgctgcctc gaactccttg aaggtgcagg tagcgaggga
     2461 tccctccctt tctggccagt acccgttgaa ggctatataa aggttctcaa ggccgaggtg
     2521 cttcgaaagc ccctctgatc tgtacaccac agggtctgat tgatcaaagt gcggtgtgtg
     2581 caccgggagc cagttaaacc tccatatgcc cctccctgca tcgtccctga aacccttttc
     2641 ctggtactct gtaacgagaa gcgcatccct gcagtgttcg caaaaggcac agaaggtctc
     2701 cttcagctct ctcccgcact ctgtgcatct gaggctaaaa tggctcatgc aatgaaacct
     2761 gataaaagtt ccatgatatc agtgattttt atccgtttgt catggttctg catcccttcg
     2821 tttacctgcg catagcacgc cggacaactg aaaataagcc tgtccgcctt cttccttacc
     2881 gcctcatcga ttgtcgatcg tccaatcgca ttggcatttt ccggatagac ctttctcgct
     2941 gctcccccgg caccgcagca cttggagttg acaccgtacc ggtcaaactc gatgagttga
     3001 acaccggaaa tgcttctgag aataatccgg ggttcttcgg taatacccac gcccctgctg
     3061 agataacagg ggtcatgata aatcagccgc tccgacaatc cctctttgat cttaagcttt
     3121 ccgcttttta gggcttctgc cgcaaactgc gtcatgtgac gtatcttaaa aggcagttcc
     3181 tgtccgtaaa atatcggcca atcccttgac atgatgttca cacagcatgg acatgaggac
     3241 aagagcagct tgacaccctt tgccttgtac gcttgcacaa gtctcttcgt caaatcttcg
     3301 agcatgccat gttgtcccga ggcaaagaga ggaaatccgc aacaaacctc ttcttccgga
     3361 ggcagcatag taaagggttc tcctattgct ttcaacacca gtacgtctcc ctttaccatg
     3421 ggttgtgccg aatacgcgcc ggtacacccg gcatagtaag cgacgtcagc cttttcggcg
     3481 actttgacat cttcgggaaa ccacgggtta aacctgtctt ttgcaggcat attgtatggg
     3541 ttgccggtat cctttattac ctcgggcatc ttggcctgtc cacctatcgg tccaaacccc
     3601 cgccttacca tttccttcct aagcatgggc cagagtctct gggtaaagat accgaccgaa
     3661 cagtgttggc cacagtcgcc acaagtagta cattcgaata ctgccttggt aaaatcttcg
     3721 aggagtttac ggtcaatgtc ttttgggttg aagagccttg ccttgaggcc ttctgttgcc
     3781 tttatgaact ccctaaagac tcggatcttc tcaggaggcg atatcgaagg tttactgttg
     3841 acgtcctgga taggacagaa tttcaaacat tcgccgcact gcgtgcatgc gtccagctct
     3901 atgagctgca ttggggtcag gtggcttgtg aaagtgtttc ccttcctcat tccctttttg
     3961 taccggaaag acagcattcc tttcgtccgg cggtcagcat tttaagcttt ccactatgtt
     4021 aaaatagaaa ttatctcttt gtaaacgata tcgtatgaga ccgggcatag ttaaccatta
     4081 cgtgcaatca tgatagacat acccgcggtc aattatccga aaccatggct tctgttctgg
     4141 gccgtggtga tactttccca cgggtatatg gactatgcgc tctaccggaa gtggattagt
     4201 tggaaccggg ggataacgca aactgcaacg accagtggta gatggggccg tgcattgaaa
     4261 atatgggcag ctgaggtctt tctccagcgt caactgttcg gactctcttc tttccgctgg
     4321 ctcatccatc ttttaatctt cttcggattt ctcgcccttg cgttcctctc tctctccacg
     4381 tttttcctca aactttcaca ttatcttggc gtcgagagca gcgtgacgca ttatttcctc
     4441 aacggcagtg gatatatttt catcaaggtt tggggcgaca gtttcggtct cgcccttctt
     4501 ctcggactta ttatagccag tgtgcgaagg tttttgttcc gcccggccca gcagatcagc
     4561 ggtcagatgg atgtcttcct tctctttttt cttctctggc ttaccctttc cgggttcatg
     4621 ctggagggac tgcgtctggc ccttgtgcct gtggagctcg cacggtgctc ctttatcggg
     4681 tgcttcttta tcccccccgg aacctatacg cttgaggaat tgcagccctg gctcactctc
     4741 ctctgggtgc tccattcttt cactggcgtg ggtatccttg tctatttgcc acacagcaaa
     4801 cttttgcaca gcatactctc tcctctggtc attgcaatga atgcccatga agaacaggaa
     4861 aggaaggacc tctattggcc cgacatagca aaacacaggg cgacaagatt gcccagggac
     4921 tgacccgcag acagctcatt gagctggaag cctgtgcgcg gtgcggcgag tgtcaggtct
     4981 ggtgtccggt ctatgctcag gataaacggg agtgcattac tgcccggggc aagctccaat
     5041 cactgcgacg gctagtgaaa gaaacgctcc ccgaagatga gaagcatgaa tttctggaag
     5101 ggctgtatga atgctccgca tgcgggcagt gtcatatcgt ctgcccggtg cgcatcaata
     5161 cccctgaact ctgggagcag tcccgtctgt ccctcgcaaa tgcgggcatc ccgcagccgg
     5221 agagccagct caagcagctc gcgaccatca aggaattcaa taattccttt ggaaaacctc
     5281 agcaggagcg ggggctctgg gccaagacgg catgggaggc aggattgctg aagtcacccg
     5341 tgccgctctg gcgcgatcat cccacgtccc tgatctattt tgccggatgc actgcaagct
     5401 ttgagcttgc catgcaatcg gtggctgtcc agaccgcccg tctgctccag gaagcgggag
     5461 tggagttctc ggtcttgggg gaagacgaac cctgctgcgt cagtaaattg cggaggatgg
     5521 gagaccccga tttctctttg gaggcgaaga aacgcttgaa tcaatttgat agaatgggaa
     5581 ttgccacggt ggtcgcttcg tgtgccggtt gttttaaagg actccattca gactattcaa
     5641 agctgtggcc tgaaagaaca cgggttatac acctcaccca gttcctggat cacctgatac
     5701 gggaacgaaa cctgcgtttg gtgcatgaag tacccttggt ggttacttac catgacccct
     5761 gccatctcgg ccgccataac cgtatctatg aagaaccaag gagcattttg aaggcaatac
     5821 ccggattgag gctcgtggag atgtcgcgcc atcgtgcctt ttcctcctgc tgcggtatgg
     5881 gcggaggatt gaaagtggca aattccggaa ttcagcacaa gatgggggcc gagagggtcc
     5941 gggaggcgga atccacagga gctgaggcaa ttgtcacccc ctgccagaca tgttccctcg
     6001 gtctcttaaa tggggtggac gaggctagtt caggtatgaa ggtttatcat ctcaatgaaa
     6061 taattgtccg gtcggtctgt cctgaggtta ccagcgaaga gatactaagc gcctttgtca
     6121 gggcagccgt ccggtaattc tttcagggca tttacctctt ttccccatgc agcagtctcc
     6181 ttaattcctc ctccctctgt cgtgcgtcca gcataacaag aggtgcggca aatatatggg
     6241 tggggacata gagaataacg acaaggacaa gggaaaaatg cataaggaac aggaaatcta
     6301 acggcactga ctgggggctt aggcccagga tccccagcac gaagcccttg accgccacga
     6361 ggtctgcctt gaagacggtt ctcagaagaa tcccggtcat gctcagaaga agcaggagga
     6421 tgaggaggaa gaagttgtat gatgggaaat atccctcttc cctgttcaat ttgactgtga
     6481 ggatatagat gagcgagagg gggaggagat agcctgcaaa gatcccgaag ggctgcagaa
     6541 aagcgaccca tcccggcaca ggacttagga aaaaaaccag gtgtcttagc acgaccagga
     6601 caaaagtgca gtgaaagagc cactccccta tccagagcag gtcatttgta ttgaggagac
     6661 gtgaaagaaa cagtatatcg ccagcaactt tcatgaccag gcggggagtg aaagaaggag
     6721 aaggagcata ccctctgacg gttcttgctg ccttccatag tgtcaggagc ttgccacaca
     6781 acaggatgaa gaaaatcccc aggactgcat aggtgctcag ggtcaagaga attcccatac
     6841 ccaccttgtt attctcagct cttcttctgt cttgccatat gacgcggaaa catcaggcgc
     6901 ctcatatctc cgacaggtcc tgcacatggg tgaacatgcc gtttatgctc cttgtctttt
     6961 ccctctctcc cgcaaggaag agaagaatct ctttccttat ggcgttcagt tcagtgctcg
     7021 tcctgtccct cggccggggg atatcgaccc tcaatacctt tccgacctcc gcaggccggg
     7081 aggtaaaaca gatgatctca tcaccaagaa ataccgcctc ttcgacatta tgggtgacga
     7141 agatcaccgt ctttcccgtc ttctcccata gatggagcag agccacctgc atatagttcc
     7201 ttgtctggca gtcaagggag acaaaaggtt cgtccatgag caggactctg ggctccacca
     7261 ccagtgccat ggccagggca accttctgct tcattccccc tgatatctct ttcgggtaga
     7321 agtccctgaa gtcctggagt tcaacaagct tcagagcggc aaatgccttc tgtctcctct
     7381 gctcatgggg gacccctgcg acctcaagcc cgaactccac gttcctgatt acggtcctcc
     7441 atggaaggag cgccggctcc tggaagatat acccgatctc cctcaccggc gatgtgatct
     7501 ccttatcccc aagcagaacc tttcccctgt cagggctctc gagccccgcg atcatcctca
     7561 ggagcgttgt cttcccgcac ccgctgggac cgatcacaca cacaaatctg ccctctggga
     7621 ccgtaaacga cacctccctt atggcatcaa tccttccgcc ttctgtggga ctgctgaatg
     7681 atttatggag gtctacgacg ttcagttcca ggactatctc catttcagga tcttatgttc
     7741 catctgcctg atgccaaaat ccatagcata tcctatcatc cctatgacaa ccattcccgc
     7801 gataatctgg tcaggccgct ggatgtcccg tgcggtcatg atcatatacc cgaggccata
     7861 tccgctcttt actcctgtga attccgcggc cacaagggtc atccatccga taccgagtgc
     7921 gatcctaacc cccgtgataa tggatggcat ggctcccggc aggagaacct tggtaaaaag
     7981 cgccctcttt tttgcgccga ggataatgga cgattcgatg agtctcctgt taatggagag
     8041 gacccctccg atggtattca atagtatggg gaaaaaggcc cccagaaaaa tgatgaatga
     8101 cgaagacctg tctccaatac caaaccagag gatcgcaata gggacccatg ccaggggagg
     8161 gatcggcctt attatttcca caaagggggt gaggtaattc cttgcccgtg tccagtatcc
     8221 catggcaatg cccagaggca gggcagtgac aatggctatg gctacgccgc tcaagaccct
     8281 cttcaggctt gctatgcagt gcccggcgag ggcgtatcca ggaggaagcc cctgggtgag
     8341 gagttcggtg aaacccagga ttatattcac cggggaagga agtctgttgc cggggataac
     8401 cccggaaaag gagagcacct gccagattac cacaacacac aagattggaa ggtatctcat
     8461 agacctgcag agccgttgaa caatcctttt cccccctcaa tcatatttga gacagcgcaa
     8521 gtctcgtaac acatcattgg acgacttcct taatcgcctt cagatcaagg tattcacgtg
     8581 caaactcatc cacattcatt gacttcacat acccgaactt cagaagatat cttacatact
     8641 ccttaatgtc atcttccttt gcgaaatagt ggtactggat atggctaatg gcctcacgca
     8701 cagtggcctc atccatcccg gtatatttca tgcctatcct aagggcatct tccggattct
     8761 tctttatata ggctgtggcc cttatatggg ccttcagaaa agattttact acctgggggt
     8821 tctctttata aaaactatca tcaactgcca cgacacagca gggatggtcc ttccatatca
     8881 tgggtgacga aataaggacc ctcccgattc ccatggtaac tgcctttgac ggatggggtt
     8941 cccacgcgat aaaggcatcg atctgttttg cgttgagagc cacgatcatc tccgggggtt
     9001 ttatcgcgat gatgttcacc ttctttgggt ctgcccctgc tgcttccagg gcctttctca
     9061 ggagaaaatc ctggacactc gaatatcccg gaatggctac ggtcttcccc gctaggtctc
     9121 ttacttcttt tattgctgaa tccttcctta ccaccaccga agacccttcc gcgtttgcca
     9181 gggcaactgc cctgatgcgg gctgacctgt ttgcaagccc ggtggcactg ggcgcaacac
     9241 cgagataccc catgtccagg ctcctggaag caaaggcagt catctcttcg ggtccggcgt
     9301 tgaatatgcc ggcaatttca acattgaccc cttcttccct gaaaaacccc ttgtccatag
     9361 cgacccaggc agcaaggtga tggaggtcgt tcctcagata cccgaggcgg acatccctgg
     9421 aagcggccat ggaaatgagc gcgcccagaa ggaccataac aagtgatgcc accaccgcaa
     9481 cctttttcat ccatgcctcc tcaatgtcaa ttccctggtt tcatggagta tgcattcttg
     9541 ggcagaagaa gggttatgtg ccggagtcct tctctgtctt cacaaacgtg atgctcacgt
     9601 ccctggggag attcagccct gcttccactt caagggcctt gaggattccc gaaggcacag
     9661 ggcaggcgac atgtctcagg cacgtcgccc cctttttgta taccgggttt tcctggagcc
     9721 ttttgaacgc atccatcatt gtgagctccg ggatttcctt tcccagcttt tccgccatct
     9781 cgcattccgt cgttatcctc accgagaaga tcttcccttc tttcttctcc acttcaacag
     9841 tgaccagcat tccgcacgcc ccggggttta ttagcacctt tgtcatgacg gtacgggttt
     9901 tcccctcacc cttttatgct cgatgcgatg ggcgccttct gctcttccag catcgccttg
     9961 agatttgggt atttgtcctt catgtgagga atgtgcatgg cctgcatgaa ctccttctca
    10021 aaagccggct ctgtcgcgat ctccacgaac tggacccacc gcgcacgctc gttcgcctcg
    10081 cgacgtttat ccttattgat aagcgccatt attgcgccat ctcccgcagc gtttccgaca
    10141 acaatgacct tgtcgatggg cacatcgggg aacatgccga gggtcaggga ggcctccctg
    10201 tctatatggc tgccgaatgc accggccagt tcgaccctgt cgagcttggt gacccccatc
    10261 ttctgcatca tcaatttggc tccggtatac agcgccccct tcgcgagctg gagcgcacgg
    10321 acatctccct gagtcacggt gatatcggca ttaatagatg tttcttcggc ccatgcgagg
    10381 acgtactcag gttttccgtc aacacccgat ctcaccctgg ttgtccccag attcatcacg
    10441 aatttgccgg tcttgtctat gatgcccgct ttaaacatct cggcaatgac atcgataatg
    10501 cctgaaccgc agatgccttt tgcgttgatc ttgccttcga tgtgggtgtg ccagtccgcc
    10561 tttccgataa ccttgtattg cggttccctg gtcacgggat cgattttcac cttctcaatg
    10621 gccccggggg ccgccctcat tccgaactta atctgggctc cttcaaatgc cggtcctgtg
    10681 gcgcaggaag tggagcagac cctgtccttg tttccgagaa gcagttcacc gttcgtaccg
    10741 atatcgatga caagcaccat ctcatcctgg aaatactgtt cctgggcaat gagaactccc
    10801 acattgtccg cgcccacaaa cccggcttca atcggcagca catggatgta tcctgcggga
    10861 ttgatcttca gaccgaggtc cctcgccttg atgttcaacg agcgctgtac tgcagggata
    10921 aagggcgagc ggccaatgta cgtagggttc aaccccagaa agatatggtg catggcagtg
//
LOCUS       DATKOU010000050         5783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3526663, whole genome shotgun sequence.
ACCESSION   DATKOU010000050 DATKOU010000000
VERSION     DATKOU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3526663"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VMU21_02345"
     CDS             complement(<1..201)
                     /locus_tag="VMU21_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76391.1"
                     /translation="MAQLLVRDIKPETINRLKDRAKQHNRSLQGEAKLILEEAAQKMT
                     MEEFKARAQKWHKRLAGRKFGDS"
     gene            complement(278..811)
                     /locus_tag="VMU21_02350"
     CDS             complement(278..811)
                     /locus_tag="VMU21_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HUO76392.1"
                     /translation="MGSPSPPDPALLFIGTLYRTEGHFSEAREKLKASFGEIAFQSPP
                     LSWDYSDYYKEELGSPLTRTFIFFKEMINPEALAGIKLFTNDLEQQISSGGKRHINLD
                     PGYLTPYQVVLASTKKYAHRIYLSKGIYAEVTLIHKEGRYSPHLFTYRDYASDEYIQL
                     FERARILLKDRTQTTDS"
     gene            complement(820..1350)
                     /locus_tag="VMU21_02355"
     CDS             complement(820..1350)
                     /locus_tag="VMU21_02355"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUO76393.1"
                     /translation="MKAKSVIVIAIFVSAIALIIFSVKREETPKTKAAVVGLNAPGFL
                     LDDPSGKTYTLSELKGSVVFINFWATWCPPCIEEIPSIQNLYNGFKDKKEFRMVTILY
                     KDDYDKAMAFLKQNNYTLPVLIDREGKAAKAYGLTGVPETYIVDKQGVLREKVIGPAD
                     WNSTQADSLISNLLKQ"
     gene            complement(1431..3878)
                     /locus_tag="VMU21_02360"
     CDS             complement(1431..3878)
                     /locus_tag="VMU21_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HUO76394.1"
                     /translation="MFEKFTERGRKIIIYAREEAEKRQNDYLGTEHLLLALLREEDSL
                     PVAILKKMGLSIEDLYMEIERNLPSGTNILTFGDIPFTPRAKKVLELAVEEARLLGHN
                     YIGSEHLLLGLIREEEGIAGKILRAFGANLLGGRQLTINLSLRTQPHVKEKRSTTPAL
                     DEFGRDLTQLARAGKLDPVIGREDEIERVLQILGRRIKNNPVIIGEPGVGKTAIVEGL
                     AQKIVTGEIPDNLVGKRIISLDLGALIAGTKYRGQFEERLKIVMKEITQSDNVILFVD
                     ELHTLIGAGAAEGSVDASSMLKPALSRGEIQCIGATTPNEYRKHIEKDGAMERRFQPI
                     YIQPPTVPNAIDILKGLRSRYENHHRVKISDEAVEAAVKLSDRYISDRYLPDKAIDVI
                     DETGSRIKLKRNMPPDELKEMEYDLSRLSKEKNLYVKLHDLEKATTTRGEEDRLKRLY
                     DQTQRRWKEDVNREIPVVKLEDVAYTVSKMTGVPLFKLEEHESEKLVRMEEALHRRII
                     AQEEAIRAMSRAIRRSRAGLKDRRKPIGSFFFLGPTGVGKTELAKALAEFLFNDESAL
                     VKIDMSEYMERFNVSKLTGAPPGYIGYDEGGQLTEKIRKKPYSVVLFDEIEKAHPDVF
                     NILLQVLDEGVLTDSFGRKVDFKNTVIIMTSNLGARIIEKATPLGFHRKSAEDAYQHI
                     KDNVMNELKKTFNPEFLNRVDEMVVFHPLEKDHLLSIIDLLIAELNKQLLDHELFIEV
                     DNAVKEWVIEKYYQPSYGARPMRRAIQKEIEDPLSEHLLKGRFKGTHKIKAVLEANTP
                     VFIEADDAAVLSGSGVN"
     gene            complement(4035..4775)
                     /locus_tag="VMU21_02365"
     CDS             complement(4035..4775)
                     /locus_tag="VMU21_02365"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HUO76395.1"
                     /translation="MTREEVEQTLILIKPDALKNSLTGYVLSQLSEFHTGLRFAAAKI
                     AHVGQMLAEEHYAEHRGKVFYPSLIEYIMGQIHYPDEPWKRRVIALVYQGPDAVKKIR
                     DICGPTNPHVAREKRPGCIRALGTVVLLKDSTGSDVGERMDNLIHASASEGEAEREIK
                     LWFKPSDFPPFMRSFPTEVNEDNYYYFKEGGLFKRHEPGCVCLIAPGEVVWKSDSDAL
                     RLLLRGLPAPLSVGAVAAKYLINYSVET"
     gene            complement(4828..>5783)
                     /locus_tag="VMU21_02370"
     CDS             complement(4828..>5783)
                     /locus_tag="VMU21_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUO76396.1"
                     /translation="MVFGELIPKSVALSHPEEVGLRVAKPVEAFSRFATLIVKLLTFS
                     ANLLLKPFGRKTFTERAYVTEEEVKMLIMEGGEQGVFEPTEQELIHSVFEFTDTSARE
                     VMVPSTRMVNIGLNMSVDEIKFTIAEEQFSRYPVIGKDLNDIRGILYAKDFLNALAKG
                     DADVRRLMKPPFFIPETMKISNLLREMQKKRVHMALVIDEYGGVSGLVTLEDLIEEIV
                     GEIRDEYDVESPVIQLSDGTLLIDAAISVRDLEDDYQIEIPESTEYETLGGFLLTSLQ
                     RIAKIGDVVEIESKKITVSEMVGQRIAKVKLEKLPEVASER"
BASE COUNT         1294 a         1532 c         1346 g         1611 t
ORIGIN      
        1 gctgtcgccg aatttcctgc cagccaaacg tttatgccat ttttgcgctc ttgccttgaa
       61 ttcttccatc gtcatctttt gtgcggcctc ttcaagaata agctttgcct caccttgcaa
      121 agagcgatta tgctgttttg cgcggtcttt aagccgattg atggtttcag gtttgatgtc
      181 tcttacaagc agttgagcca ttggatgcct ccttattccc ctgtgatatc ataatgatag
      241 cagaagggaa ttaaataagc aactgccaag ggtgttctta agagtcagtc gtttgcgttc
      301 ggtcttttaa gagaatcctg gcgcgttcga agagctgaat atactcatcg ctggcataat
      361 ctctgtacgt aaacaggtgg ggagaatatc tgccttcctt gtggataagc gtaacctccg
      421 cataaatccc tttgctgagg taaatgcggt gggcgtattt ctttgtagat gcgagcacca
      481 cttgataagg ggtgagatat ccgggatcga ggttgatgtg cctctttccg cctgaagata
      541 tctgctgctc cagatcattt gtaaagagct ttataccagc aagtgcttcc gggttgatca
      601 tctctttgaa aaagatgaat gtccttgtga ggggagaacc aagctcttcc ttatagtaat
      661 cagaatagtc ccaggaaaga ggcggacttt gaaaagctat ttcgccgaaa gaggctttca
      721 gcttctccct tgcctcagag aagtgtcctt cggtgcggta aagtgttccg ataaagagaa
      781 gtgcaggatc aggaggggaa gggcttccca cgttcaaagt tactgtttga gaaggttaga
      841 gataagggag tcggcctggg ttgaattcca gtcagcaggc cctatcactt tttccctcag
      901 gaccccctgt ttgtccacga tatacgtttc gggtacgccg gtcagcccat aggcctttgc
      961 cgcttttccc tcgcggtcga tcaagacagg gagcgtatag ttattttgct tcaggaatgc
     1021 catggccttg tcataatcat ccttgtagag gatggtgacc atccggaatt cctttttgtc
     1081 cttaaatcca ttatagagat tctgtatgga gggaatctcc tcaatgcagg gcgggcacca
     1141 cgttgcccag aaattgataa agactaccga accctttagt tcagagagag tatatgtctt
     1201 acccgaggga tcatcaagca gaaatcctgg cgcattgaga ccgacaacag ctgcctttgt
     1261 ctttggtgtt tcctctctct tgacgctgaa aatgatgagt gctatggcgg aaacgaaaat
     1321 ggcaataact atgaccgact ttgctttcaa tagttaactc ctcatcttca ctgcgatttt
     1381 ttccaagatg cccaaaagga ggctaagggc tggattctgg gtgtctctct ttaattgacc
     1441 ccggaccctg acagcaccgc cgcatcatcg gcctcgataa agaccggcgt gttggcctca
     1501 agaacagcct taattttatg ggtcccttta aacctgcctt tcagcaagtg ttccgagagg
     1561 gggtcttcga tctctttttg tattgccctt ctcatcggtc tcgcaccata ggaaggctga
     1621 tagtatttct cgatgaccca ttccttcacc gcgttgtcta cttcgatgaa gagctcatgg
     1681 tcaaggagct gcttattcaa ttcggcaatg agaaggtcga taatggacag gagatggtcc
     1741 ttctcaagcg gatggaaaac taccatctca tccacccggt tgaggaattc gggattgaag
     1801 gtcttcttga gctcattcat gacattgtcc ttaatatgct gataggcatc ttcagcgctc
     1861 tttctgtgga agccaagagg ggtcgccttc tcgatgatac gcgcacccag attggaggtc
     1921 ataatgatca ccgtattctt gaaatcaacc tttcttccga agctgtcggt gagcactccc
     1981 tcgtccagaa cctgcagcag aatgttaaat acatcgggat gggccttctc gatctcgtca
     2041 aacagcacca cggaatacgg ttttttcctt atcttttctg tgagctgtcc cccctcatca
     2101 tagcctatat acccgggagg tgcgccggtc agttttgata cgttgaacct ctccatatat
     2161 tcagacatat caatcttcac aagcgcgctc tcatcgttga agaggaactc tgccagggcc
     2221 tttgccagtt ctgtttttcc cacaccagtc ggcccgagaa aaaagaagga ccctatgggc
     2281 ttccttctgt ctttcagtcc cgcacgggac ctcctgatgg ccctcgacat cgccctgatg
     2341 gcctcctcct gggcaatgat cctcctgtgg agcgcttcct ccatcctcac gagcttctcc
     2401 gactcatgct cttccagttt aaaaagagga acgcccgtca tcttcgagac ggtatacgcc
     2461 acgtcctcca gtttcactac agggatttcc ctgttcacat cctctttcca ccttcgctgg
     2521 gtctggtcat ataacctctt gagtctgtct tcctccccac gggtagtcgt cgccttctcg
     2581 aggtcatgga gcttcacgta gagattcttt tcctttgaga gcctgctgag atcgtattcc
     2641 atctccttca gctcatcggg aggcatattc cgtttcagct tgattcgtga accggtctca
     2701 tcaatgacat cgatcgcctt gtcagggagg tacctgtccg agatgtaccg gtccgaaagc
     2761 tttaccgcgg cttcaacagc ctcgtcactg attttcacac ggtgatggtt ctcgtacctg
     2821 ctccgcaatc ccttgagaat atcgatggca ttaggtacag tgggcggctg gatataaata
     2881 ggttggaatc tccgttccat tgccccgtct ttctcaatat gttttctgta ctcgttggga
     2941 gtagtagcgc ctatacactg tatttcgcca cgggacaggg caggctttaa catgctggag
     3001 gcgtccacag acccttccgc tgcgcccgct ccgataaggg tatgcaattc atcaacaaag
     3061 aggatcacat tgtcggactg ggtgatctcc ttcattacga tcttcagcct ttcctcgaat
     3121 tgccctctgt attttgtccc tgcgatgagc gcaccgagat ccagggatat aatacgtttc
     3181 cccacaaggt tgtcggggat ttcccctgtc actatctttt gcgcaagtcc ttcaacaatt
     3241 gcggtcttcc ctacccccgg ctccccgatg atcacagggt tattctttat tcttctgccg
     3301 agtatctgga gtaccctttc gatctcatcc tccctgccga ttacgggatc aagcttccct
     3361 gccctcgcca actgtgtcag gtcccgtcca aactcatcca gtgcaggtgt ggtgctcctc
     3421 ttttccttca catggggctg tgtcctcagg gagaggttta tggtaagctg acgcccaccc
     3481 aggagatttg caccaaatgc cctgagtatc tttcccgcga ttccttcttc ttcccggata
     3541 agaccgagca gaaggtgttc gcttccaata tagttatggc ccagcaaccg tgcctcttcc
     3601 accgcgagtt ccaggacctt ttttgcccgg ggggtgaagg ggatatcccc gaaggtgagg
     3661 atattcgtgc cggaagggag gttcctctca atctccatgt agaggtcttc aatggagaga
     3721 cccatcttct tgagtattgc cacgggaaga ctgtcctctt ctctcaggag ggccaagaga
     3781 agatgctcgg ttccgaggta atcgttctgc ctcttttcgg cctcctccct cgcataaatg
     3841 attatctttc ttcctctttc tgtaaacttc tcaaacattg cgtcctccta gaaaaataat
     3901 aatgcttttc cctaccgaat gtcaactaaa ggaagctcct attgggtcgt ctaaaggaga
     3961 gattacaatg gcttatggga aacgccggga acgcaggcca tggatgtgta atgcaagata
     4021 ccgtagcctc ttctttatgt ctccacacta taattaatga gatatttggc tgccactgca
     4081 cccacagaaa ggggagcagg caaccctcgt agcagcaaac gcaacgcatc agaatcagac
     4141 ttccaaacca cttcaccggg agcaatcagg catacacaac ctggttcatg tctcttaaaa
     4201 agcccaccct ctttgaaata ataatagttg tcctcattta cctcggtagg gaacgaacgc
     4261 atgaacgggg gaaaatcgct gggcttgaac cagagtttta tctctcgttc ggcctcgcct
     4321 tcggaagcag aagcgtggat gaggttatcc attctttcgc caacatcgct tcctgtactg
     4381 tccttaagaa ggacaactgt gcccagcgcg cggatgcatc ctggcctctt ttcgcgggct
     4441 acgtggggat ttgtgggtcc gcagatatcc cgtatctttt tcactgcatc aggaccctga
     4501 tacacaagcg cgattactct ccgcttccag ggttcgtcag gatagtgaat ctgccccatg
     4561 atgtattcta tgagcgaagg ataaaagacc ttacccctgt gttcggcata gtgttcctcg
     4621 gcaagcatct gccctacgtg ggctatcttt gctgccgcaa agcgcagacc ggtatgaaat
     4681 tccgagagct gagaaagcac atatcctgtt aaggaattct taagggcatc aggtttgata
     4741 agtatgagtg tctgctcgac ttcttctctg gtcatgtgag ggttcctttc atatcacaaa
     4801 agtattttgc tctgacagaa tgtggcccta tcgttcggaa gccacctcgg gcagcttttc
     4861 cagtttcacc ttggcaatcc tctggcccac catctcggat acggttattt tcttgctttc
     4921 aatctctacc acatcaccga tcttcgctat cctctgcagt gaggtcaaca ggaagcctcc
     4981 cagggtttcg tactccgtgg attcggggat ttctatctgg taatcatctt ccaggtctct
     5041 cacgctgatg gccgcgtcaa tgaggagtgt gccgtcactg agctggatca cgggactttc
     5101 cacatcgtat tcgtctcgta tttcacccac aatctcttct atgaggtcct caagggtcac
     5161 aaggccggac actcctccat attcgtcgat cactagggcc atatgaactc ttttcttctg
     5221 catctcccgc aagaggttgc tgatcttcat tgtctcaggt atgaagaacg gaggcttcat
     5281 aagcctgcgt acgtccgcat ccccttttgc gagcgcattg agaaaatcct ttgcgtagag
     5341 aatacccctg atatcattca ggtccttgcc gatcacggga tagcgggaaa actgctcttc
     5401 agcgatggta aattttattt catccacgga catattcagc cctatattca ccatccgtgt
     5461 ggagggaacc atgacctctc tggctgacgt gtccgtgaat tcaaacacgc tgtgaataag
     5521 ctcctgctcc gtgggttcaa agacaccctg ctcgcccccc tccataataa gcatcttgac
     5581 ctcctcctca gtcacatacg ccctttcggt aaaggtcttc ctcccgaatg gcttgagcag
     5641 cagattcgca ctgaaggtga gaagcttcac aatgagagtg gcgaaacgcg agaatgcctc
     5701 gacaggtttt gcgactctga gccccacctc ctcaggatgc gaaagtgcca cggatttcgg
     5761 gataagctca ccgaagacca tgg
//
LOCUS       DATKOU010000051        12975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1218513, whole genome shotgun sequence.
ACCESSION   DATKOU010000051 DATKOU010000000
VERSION     DATKOU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218513"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1050)
                     /locus_tag="VMU21_02375"
     CDS             complement(70..1050)
                     /locus_tag="VMU21_02375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HUO76397.1"
                     /translation="MKSGSNLERVIASGQPAVTAELGPPMSADPHEVQKKAQILKGHC
                     DAANITDCQTAVVRISSIAAAVLALREGLEPVMQMTCRDRNRIAIQADLLGGAALGLK
                     NCLCIAGDHQKFGAAGKLKGHPGAKNVYDVDTTQLVGILTKMRDEGMQEGGDKLEVAP
                     KFFIGASWTPMGDPLDFRPINLLKKVNAGADFIQTQGIYDIEAFRSQMEKIRNMGLHE
                     RTAILGGIIVPKSGMMLKYMDASVAGVSVPKFLIDRMNKAKNEAGDDKKKARELQEAE
                     GIKIAVELIQQVLETPGIKGVHIQAIEWETAMPEIVKAAGLYPRPQIPEA"
     gene            complement(1065..1763)
                     /locus_tag="VMU21_02380"
     CDS             complement(1065..1763)
                     /locus_tag="VMU21_02380"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HUO76398.1"
                     /translation="MIVGSQKPFEEIWDMVKDFKKVLVFGCNTCVAICHAGGGKEAEI
                     IASMIRMKATQEGKEMEVKHAGIERHCEPEFFEPAMEEMKTYDLVLSTACGVGVNFLS
                     DLIGGEIPVYPGINTSFYGGVPKAGVFTELCAGCGNCILHLTGGICPVARCSKSLMNG
                     PCGGTNKGKCEVSQDVDCAWYLIVERMKKLGTLEKLSKIEPPRNWSSGWYGGPRRVVV
                     EHIADLEEQEKKEA"
     gene            complement(1760..2269)
                     /locus_tag="VMU21_02385"
     CDS             complement(1760..2269)
                     /locus_tag="VMU21_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2003..2005),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HUO76399.1"
                     /translation="MTTVAEPAAGATVSSIEKPHETPPGWEPNVLAFACHYCAFAAAD
                     LAGVMRLSYPTNVKIIRLPCTGKLDHIHILRAFERGVDGAFAAGULQGQCHYLEGNTF
                     AARRIQYVKKLLKNLGIDPARLEMYNLSAAMGPKWAELCTEFTERIRTLGPSPIWLAL
                     SRRKEEINP"
     gene            complement(2298..5345)
                     /locus_tag="VMU21_02390"
     CDS             complement(2298..5345)
                     /locus_tag="VMU21_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO76400.1"
                     /translation="MNGNGSSPKRGSVLILGGGIGGMQAALDLADSGFKVHMVQKEPS
                     IGGIMVKLDKTFPTGDCSMCMISPKMVEAGRHLNIDIHTLAEVVSVEGEAGDFKVKVK
                     LAPRYVDSDKCTGCGDCEAKCPKKVSSEFDQGLGQRKAIYSLFPQAVPNTRAIDAAKC
                     IYLTTGKCRACEKVCKAKAINYEDKGKEMELNVGSIILSPGLEPYDARVRGELGLGRW
                     KNVVSSIQFERILSASGPYKGVIQRPSDEKHPHKVAWIQCVGSRDFHNANPWCSSVCC
                     MYATKQAIIAKEHDARIEPSIFFMEMRAYGKDFDKYVERAKNEYNVRYQRAMVSAIRE
                     EPETGNLILRYALEDGKLMDETFDMVVLSVGLEPHKNAEQVAKTFGIDINQYRFPLTS
                     PLKPIETSREGVFVTGIYQGPKDIPETVMQGSAVAGRTMALLAEARGTETVTKELPPE
                     KDVSHEEPRIGVFVCHCGTNISSTVEINSVVEAMKRQPGVVYVTDTIYACAQDTQDVL
                     KQTIKDQNLNRVVIASCTPRTHEKLFQETIREAGLNKYLFDLADIREQCSWCHKGQNE
                     AATKKAIQVVKMAVAKSRLQEPLKTESVGVTPACLIVGGGIAGMTTALSLADQGFQIS
                     IVEKETELGGLLKNLHRTLEGNDVQEFLKSKISEVLVHPRINIYTGVEVKKTDGFVGN
                     YKTTLTDGTVIDHGAVVLTTGGVEYEPTEYLYKQNDHVMTQRELEKRLASGETPKSKE
                     RFVMIQCVGSREEPYQYCSRICCQDAIKNAIAIKERTPDAQVFILYRDMRTYGLREDY
                     YKKAREMGVIFVRYEVDKKPRVEASGDKIRVSTWDYMLNKEVVVNADQLVLSTGLRPH
                     PTTEHVGETYKVTRNPDGYFLEAHVKLRPVDFPSEGLFVAGLAHAPKNLDETLSQALA
                     AAGRAGVILSHERLAVSGIVAKHKRELCMSCLSCLRVCPFGSPYIAEDGKISHNEIKC
                     MGCGICAGICPAKAFQVNNFRDDQIIAMIDAAVESEEPGFIGA"
     gene            complement(5342..6217)
                     /locus_tag="VMU21_02395"
     CDS             complement(5342..6217)
                     /locus_tag="VMU21_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HUO76401.1"
                     /translation="MEIAYYPGCSLHASSQLYDIQNKLVFKKLNVNLREIEDWNCCGA
                     TSASKTNDFLSVALPARNLGIADATGLSEILIPCSSCYSRTLVSQKRLQEDTPLRDTI
                     NEELSHKVKGKIKVSSILEVLLPRVTSGELSEKATRKLEGLKPACYYGCLLTRFPSDV
                     AVPDDVENPQGMEKVCKALGAEPIDWGYKTDCCGASAAVNDADVSLGLMAKILKDAAA
                     RGANCFVTTCPMCQFNMDAYQDQVAEKYRIRERLPVYFITELMGIAMGMSPQAMQVDR
                     HFVDSMRLLKELKLT"
     gene            complement(6249..6851)
                     /locus_tag="VMU21_02400"
     CDS             complement(6249..6851)
                     /locus_tag="VMU21_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO76402.1"
                     /translation="MSDKTSVLELDVSQDLVKEVMDRSGQNLLTCYQCRRCAAGCPVG
                     DVTGVTPDRLIRMILFGEREDALGNLLVWECVACYTCGTRCPNNIQTARITETLKQMS
                     KEAHMEPLTPKVGFFHDAFMTSASNAGRLNELEFMRLYETKSLQTELKERGVKGAMQE
                     MKSQGKLGLAMMKRKRMHFGSEKVKGIAEVKALSKKAKSR"
     gene            complement(6970..7878)
                     /gene="folD"
                     /locus_tag="VMU21_02405"
     CDS             complement(6970..7878)
                     /gene="folD"
                     /locus_tag="VMU21_02405"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HUO76403.1"
                     /translation="MSAKIISGTDIAAQIRAELKKQVTEMKEKHGVVPGLVTILVGKN
                     PASVSYVTGKHKAADEIGFFSIQDDQPESISEVDLLKLIDKYNKDPKIHGILVQLPLP
                     KHIDEKKVLNAIDPDKDVDGFHPVNVGRLMIGGDEAKFLPCTPAGIQELIVRSGFETS
                     GAEVVVVGRSNIVGKPIANIMLQKGRGANSTVTIVHTGSKNIDAHCKRADILIVAAGV
                     PGLVKPEWIKPGACVIDVGVNRVGEKISEKTGKPVPILKGDVDFDKAKEIAGAITPVP
                     GGVGPMTITMLMKNTVVSAKMHAGIK"
     gene            complement(7965..8528)
                     /gene="folE"
                     /locus_tag="VMU21_02410"
     CDS             complement(7965..8528)
                     /gene="folE"
                     /locus_tag="VMU21_02410"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HUO76404.1"
                     /translation="MDIRKIQKGVRLIIEGIGEDPERPGLRRTPERVAKMYQEIFSGL
                     AGSPEDILKPMEGEKHDEMVLLKDIPFYSVCEHHLLPFAGKAHIAYIPAGGRIVGLSA
                     LAEALEVFAKRPQVQERLTAQLADIIMKKLNPKGAMVVIDAEHLCLSMRGIKKPGART
                     VTSAVRGLFRSKESTRQELLELVKKRD"
     gene            complement(8615..9043)
                     /locus_tag="VMU21_02415"
     CDS             complement(8615..9043)
                     /locus_tag="VMU21_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HUO76405.1"
                     /translation="MEQEIFREFLSRKGLKLTKERQRILSEVFSRHGHFNPEELLTTM
                     RRKHIKVSKASIYRTLPLLVESGLIEQVVRNDKHAHYEHIFGHEHHDHMICIKCGKVM
                     EVFSPKLEAIQEELCSANRFMGITHTLEIKGYCQGCRQVS"
     gene            complement(9075..9653)
                     /locus_tag="VMU21_02420"
     CDS             complement(9075..9653)
                     /locus_tag="VMU21_02420"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HUO76406.1"
                     /translation="MEGKSRIRQDVLRKRDSLDPDIRKAKDSLIKEQLFSLAEFQQAP
                     VLFFFSSFRSEVSTSAQIAEALSLGKRVILPSVDSLNKELRLYEIKQLSELSAGYMGI
                     PEPAVAEERERDINDVTLVIMPGAAFDPKGNRLGYGGGYYDRLLSRLRRRIPLVALAY
                     EEQLVDSLPTGPHDIRVHMIVTDQRVIHCRKD"
     gene            complement(9657..10430)
                     /locus_tag="VMU21_02425"
     CDS             complement(9657..10430)
                     /locus_tag="VMU21_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HUO76407.1"
                     /translation="MKIAVTGKGGVGKTTLSAVLSHLYASEGKKVIAVDADPDANLAA
                     ALGVPPEAVSRIRPLAEMTDLIEERMGARPGKSGGVFKLNPKVDDIPEEFGYRFNNIT
                     LLVTGKSKEAASGCYCPENVFLRRLLKHLVVNRDEIVIVDMEAGIEHLTRGTTEAVDA
                     FIVVVEPGQRSIQTAHTVKEMAKGLGVKKVFIVANKVRGEEDLAFIRKQLSDAPLLGF
                     ISFSHEIMECDIAGVTPSTIKGAVADIRKIKEVLEQSTG"
     gene            10640..11779
                     /locus_tag="VMU21_02430"
     CDS             10640..11779
                     /locus_tag="VMU21_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUO76408.1"
                     /translation="MSLVGRLEDLALSDIFQILSIGKKTGTLIIRSEKGNAFIVFKNG
                     LIVRAETNMIEGNIGHDLLKKNVVKEQVFTMGLAVKKTLPQKPIAEILFELGSVSKEA
                     LDKAAKKRIEKVVYHLLLANQGDFRFELDTHDVGSIANVEDVGWEVSKGISPEYLLME
                     GARVHDEMMQKESLEGSLAEETEGETWEEDWGRRGTVPERKDISALKALTQELRFPNS
                     TSEITLLILRFASDIFHRGVLFMVGETELAGLGQFGLDIKGADEKIRETVLTYAESPF
                     LKGIIEAGQSYKGRMEKDPVTEFLMGDLGGGWPIEVALFPVITEGKVVALLYCDNAAT
                     GEDFSETEGLEIFISQAGLALEKSLLQRKLQEMQKDAGKIPPASA"
     gene            11855..12226
                     /locus_tag="VMU21_02435"
     CDS             11855..12226
                     /locus_tag="VMU21_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO76409.1"
                     /translation="MKSVLIVEDSATTRAMIRAVIEDMEEDFTTLEASTGFEALKLLP
                     QGSFDLIITDINMPDVNGLELIHFVKTNPNYQHIPIVIVTTERSSEDRERGLALGASA
                     YITKPFTSEQLQEVITKILNP"
     gene            12223..>12975
                     /locus_tag="VMU21_02440"
     CDS             12223..>12975
                     /locus_tag="VMU21_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HUO76410.1"
                     /translation="MKSSKKDFIVEAEELIEEAERLLLEVQETYATGINPDTINALFR
                     NIHTVKGISGLFGFKYIADFSHAFESLLDDVRMGRIEISEEVVRFLFSNIDLLKRMIE
                     GIEEEKEQDVSGYLKGIESFRKSLKGSGGDQEVQESLGQIDSSILKVLSEFEEHRLKT
                     NIKEGRGIYLAKAVFSLADFDTSLNALTTLVKSQGEQISTLPTAADVPPDSIGFNILF
                     GSTKPLKELTQAISFPVDELIKHRAEPSPSSPP"
BASE COUNT         2903 a         3523 c         3255 g         3294 t
ORIGIN      
        1 gaggcataga acttaatgaa caaaaagggg agctgaccat ggtcagctcc cctttttgtt
       61 cattaagttc tatgcctctg gtatctgagg tcgcgggtac aggcctgctg ccttgacaat
      121 ctcgggcatt gcggtttccc attcaatggc ctggatatgc acgcccttaa tgccgggggt
      181 ctcaaggacc tgctggatca gttccactgc gatctttatc ccttcagcct cctgcagttc
      241 tctggccttc ttcttatcat cgcctgcctc attcttcgcc ttgttcatcc ggtcgataag
      301 gaacttcgga acagaaacgc cggcgaccga ggcatccatg tacttgagca tcatgccact
      361 cttcggcacg atgatgccgc caagaatcgc ggtgcgctca tggagaccca tattcctgat
      421 cttttccatc tgtgaacgga aagcctcaat atcgtagatg ccctgggtct ggataaaatc
      481 agcgcctgca ttcactttct taagaagatt gatgggcctg aaatcgaggg ggtcgcccat
      541 gggggtccag gaagcaccaa tgaaaaactt cggcgccact tccagcttgt cgcccccttc
      601 ctgcattccc tcgtcgcgca tctttgtgag gatcccgacg agctgggtgg tatcaacatc
      661 gtacacattc ttggctccgg gatgtccctt aagtttaccc gcagccccga acttctggtg
      721 gtctccggca atgcagagac agttcttgag ccccagcgca gctcctccga ggaggtctgc
      781 ctggatggcg atcctgttcc ggtcccggca ggtcatctgc atgaccggtt caagaccttc
      841 cctcagggca aggaccgcag cagcaatgct ggatatgcgg acgaccgctg tctggcagtc
      901 ggtgatattc gccgcgtcgc agtgaccttt cagtatctgc gcctttttct gcacttcatg
      961 gggatcagcg ctcatgggcg ggcccaactc ggctgttacc gcaggctgac cacttgcaat
     1021 tacccgttca agattgcttc cactcttcat cctttattcc tccactatgc ttcttttttt
     1081 tcttgttctt cgaggtctgc gatatgctca actaccaccc ggcgcggccc tccgtaccag
     1141 cctgatgacc agttccgggg tggctctatc ttggacagtt tttcgagggt gccgagtttt
     1201 ttcatcctct cgacgatgag gtaccatgca caatcaacat cctggctcac ctcacatttc
     1261 cccttgttcg tgccgccgca aggaccattc ataagggact tggagcagcg ggcaaccgga
     1321 catatgccgc ctgtcaaatg gaggatgcag ttgccgcatc ctgcgcagag ttcagtaaat
     1381 acccctgcct tggggacgcc accgtaaaac gaggtgttga tcccgggata taccggtatt
     1441 tcccccccta tcaggtccga gaggaaattc acccccacac cgcatgcggt ggagagcacg
     1501 agatcgtacg tcttcatctc ttccattgcc ggctcaaaga attcaggctc gcagtgcctc
     1561 tcgataccgg catgcttcac ctccatctcc ttgccttcct gtgtcgcctt catccggatc
     1621 atggaggcaa ttatctcggc ctcctttccg ccgccggcat ggcatatggc aacacaggta
     1681 ttgcaaccaa agacaaggac ctttttgaag tccttgacca tatcccaaat ctcttcaaag
     1741 ggtttctgag aaccgacaat catgggttaa tctcctcctt gcgtctgctg agcgcgagcc
     1801 aaatcggtga gggaccgagc gtacgaattc tttcggtaaa ttcggtgcag agctcagccc
     1861 atttcgggcc catggcagcg gacagattat acatttccaa acgggcaggg tcaatgccca
     1921 ggtttttcag aagtttcttc acatactgta tgcgccgggc agcaaaggta ttaccttcca
     1981 ggtaatggca ttggccctgc agtcagcccg ccgcaaacgc gccgtccaca ccccgttcaa
     2041 aggccctgag gatgtggatg tggtccagct tccctgtgca gggaagacgg ataatcttca
     2101 cgttcgttgg ataggagaga cgcatcacgc ctgcaaggtc agctgcggca aaggcgcagt
     2161 aatggcaggc aaaggccagt acgttcggct cccatcctgg cggcgtctca tggggcttct
     2221 cgatcgagga gaccgtggcc ccggccgctg gttcagcaac agtagtcata cttcaatcct
     2281 cctatatctc aaatttctca tgctccgata aaaccaggct cctcgctttc gaccgctgcg
     2341 tctatcatcg caatgatctg atcatcgcgg aaattgttca cctggaaggc cttggcagga
     2401 cagatccctg cgcagatgcc gcaccccata cacttgatct cattgtggga aattttgccg
     2461 tcttcagcga tatagggtga gccgaaggga cagaccctga gacagctgag gcaggacatg
     2521 cagagttccc gtttgtgctt ggcgacaatg cccgaaaccg cgagcctttc atgtgacagg
     2581 atgaccccgg cccttcccgc ggccgccagt gcctggctga gagtctcatc aaggttcttg
     2641 ggcgcgtggg ccagacctgc aacgaataat ccttcgcttg ggaaatccac aggacgcaac
     2701 ttcacatgcg cctcaaggaa gtatccgtcg ggattacgcg taactttgta tgtctcaccc
     2761 acgtgttcag tggtggggtg gggtctcagt cctgttgaca ggacaagctg gtcagcgttg
     2821 accacaacct ctttgttcag catgtagtcc catgtgctga ccctgatctt gtcacccgag
     2881 gcttctaccc tgggcttctt gtcaacttca tacctcacaa agatcacgcc catttcacgg
     2941 gcctttttgt aatagtcttc gcgaaggcca taggtgcgca tgtcacggta gaggataaag
     3001 acctgggcat cgggagtccg ttccttgatc gcaatagcgt tcttgatcgc atcctggcag
     3061 cagatacggg aacaatactg ataaggttcc tcacgcgatc ccacgcactg gatcattacg
     3121 aaccgttcct ttgattttgg tgtttcaccg ctggcgagcc tcttttccaa ctcgcgctgg
     3181 gtcatcacat gatcattctg tttgtagaga tactctgtgg gttcgtactc aactccccct
     3241 gtggtcagta ccacagcgcc gtgatcaatg actgtgccgt cagtgagggt tgtcttgtag
     3301 ttgcccacaa agccatcggt cttcttgacc tccacgccgg tatagatgtt aatgcgggga
     3361 tgcaccagca cctcggagat cttggatttc aggaactcct ggacatcatt cccctcaagg
     3421 gtgcggtgaa gatttttgag aagaccgccc agttcggtct ctttctccac aatacttatc
     3481 tggaagccct gatcagccag tgacagcgca gtggtcatac ctgcgatgcc gccaccaact
     3541 atcagacatg cgggcgtcac cccgaccgat tcggtcttga gaggctcctg cagccttgat
     3601 ttggccacag ccatcttgac aacctggatc gccttcttgg tggctgcctc gttctggcct
     3661 ttgtggcacc atgaacactg ctccctgatg tcagcaagat caaagaggta cttgttgagg
     3721 cctgcctcgc ggatcgtctc ctggaagagc ttctcgtgag tcctgggcgt gcatgaagcg
     3781 atgaccacac ggttcagatt ctggtccttg atagtctgct tgagcacgtc ctgggtgtcc
     3841 tgggcgcagg cataaatggt atctgtcacg tacacgacac caggctgcct tttcatggct
     3901 tccaccacac tgttgatctc tacggtggag gagatattgg tgccgcaatg gcagacaaac
     3961 acaccgatcc tcggctcttc gtgcgagacg tccttttcag gggggagctc tttggtcacc
     4021 gtctctgtgc cccgtgcctc tgcaagcaat gccatggtcc ttcccgcaac agcgctcccc
     4081 tgcatgactg tctcggggat gtccttgggt ccctggtaga tacctgtcac gaaaactccc
     4141 tcgcgggatg tctcgatggg cttgaggggt gacgtcagcg gaaaacggta ctgatttatg
     4201 tcaataccaa aggtcttcgc cacctgttcg gcgttcttgt gaggctcgag tcctacggac
     4261 aagaccacca tgtcaaaggt ctcgtccatc agttttccgt cctctagcgc gtaacgcaga
     4321 atcagattgc ccgtctccgg ctcttcacgg atagcagaga ccattgccct ctgataacgg
     4381 acattgtatt cgttctttgc acgttccaca tacttgtcga aatccttgcc gtaggcccgc
     4441 atctccataa agaagatgct cggctcaatg cgggcatcgt gctcctttgc gatgatggcc
     4501 tgctttgtgg catacatgca gcagacggaa gagcaccatg gatttgcgtt atgaaaatcc
     4561 ctggagccga cacactgtat ccaggcgact ttatggggat gcttttcgtc cgacggacgt
     4621 tgtatcacgc ccttgtaagg acctgacgcc gacagtatgc gctcgaattg aatggaggac
     4681 acgacgtttt tccagcgtcc caatcccagt tctcccctta cccttgcatc gtaaggctca
     4741 agccctggac tgaggatgat ggagcccacg ttgagttcca tctcctttcc cttgtcctcg
     4801 taattgatcg cctttgcctt acagaccttt tcacaggcgc ggcacttgcc ggtggtaaga
     4861 tagatacact tcgccgcatc aatggcccgg gtgttcggga ctgcctgagg gaagagagaa
     4921 tagatcgcct tacgctgacc gagtccctgg tcgaactcgc tcgagacctt cttcggacac
     4981 tttgcctcac agtctccgca tccggtgcat ttgtcggaat ccacatagcg gggggctaat
     5041 ttcaccttta ccttgaaatc cccggcctcc ccttcgaccg agaccacttc agcaagggta
     5101 tggatgtcga tgttcaggtg cctgcccgcc tccaccattt tgggtgagat catgcacatg
     5161 gagcagtcac cggtgggaaa ggtcttgtcc agcttgacca tgattcctcc gattgaaggt
     5221 tccttctgga ccatgtgcac cttgaacccg ctgtccgcaa ggtcaagggc tgcctgcatt
     5281 cccccgattc cgccgcctaa gataagaaca gacccgcgct ttggtgatga accatttccg
     5341 ttcatgtcag tttcaactcc tttaaaagtc tcattgagtc cacaaaatgc ctgtcaacct
     5401 gcattgcctg cgggctcatg cccatggcga tgcccataag ttcggtgata aaatacacag
     5461 gcagcctctc cctgatcctg tatttctccg ccacctggtc ctgatatgca tccatattga
     5521 actgacacat gggacaggta gtaacaaaac agtttgcacc ccgggcagcg gcatccttaa
     5581 gtatctttgc catcaggccc agagaaacat cggcatcatt gaccgctgca gacgcaccgc
     5641 agcagtcggt cttatacccc cagtcaatgg gctcggctcc aagggcctta cagaccttct
     5701 ccataccctg gggattttcc acatcatccg gcactgccac gtcactggga aagcgtgtca
     5761 acaggcagcc atagtaacag gcgggtttga gtccctccag tttccttgtt gccttttctg
     5821 aaagttcgcc ggaggtaacc cttgggagaa ggacctcaag gatgctggac acctttatct
     5881 ttcccttaac cttgtgagaa agttcttcat ttatcgtgtc tcttaatggt gtgtcttcct
     5941 gaagccgctt ctgggaaacc agcgtacggc tatagcagga tgagcagggg ataaggatct
     6001 cggaaagccc tgtggcatca gcaatgccca ggttgcgcgc gggaagcgca acagagagaa
     6061 aatcgttggt cttgctggct gaggtcgcac cgcagcagtt ccagtcctct atttccctga
     6121 gattgacatt caacttctta aagacgagtt tgttctggat gtcatagagc tgcgatgacg
     6181 catgcaaaga acatcccgga taataggcaa tttccatggt ctacctcttg gagtgtttca
     6241 tcaacagatt accgcgactt ggccttctta gagagggcct tgacctcggc gattcccttt
     6301 actttttctg acccgaaatg catccgtttt ctcttcatca ttgcgagtcc cagctttccc
     6361 tgactcttca tctcctgcat ggcgcccttt actccccgct ctttcagttc agtctggaga
     6421 ctctttgtct cgtataaccg catgaactca agttcattca gcctgccggc attggaggcc
     6481 gatgtcatga aggcgtcatg gaaaaagcct accttgggcg tgagcggttc catatgggct
     6541 tctttggaca tctgcttgag cgtttcggtg atacgcgctg tctgaatatt gttcgggcag
     6601 cgcgtgccgc aggtgtaaca ggcgacgcac tcccagacaa gaaggttacc gagcgcgtcc
     6661 tccctctctc cgaagagaat catccttatc agacggtcag gggtaacacc cgtgacatca
     6721 cccactgggc agcccgcagc gcatcgccgg cattggtagc aggtcagcag gttctggcct
     6781 gagcggtcca taacttcctt tacaaggtct tgagagacgt ctagctctaa cacgcttgtc
     6841 ttgtctgaca tctagatctc cttaattcat taggtcttag gagtttaact aatttttaca
     6901 ctatttatta tataatcttt gccctattct ttgcaactat tcatcccttt ggaaagaacc
     6961 gcttatcggt cattttatac cggcatgcat ctttgcggat acgaccgtat tcttcatgag
     7021 catggttatg gtcattggtc cgacaccgcc aggcacaggg gtgatggcac cggctatctc
     7081 ctttgcctta tcaaaatcaa cgtcgccctt gagaatgggt accggcttgc cggtcttttc
     7141 acttatcttt tcgcccacac gattcacccc cacatcaatg acacaggcgc ctggcttgat
     7201 ccattccggc ttcacaagac cgggcacccc ggcggcaaca atgaggatgt cagcgcgctt
     7261 gcaatgcgca tcaatgtttt tcgaaccggt atgtactatg gtaaccgttg agttcgcgcc
     7321 cctacctttc tggagcataa tgttggcaat gggtttgccc actatgtttg agcggcccac
     7381 caccaccact tcagcgcccg aggtttcaaa ccctgagcgg acgatgagct cctgaatgcc
     7441 ggctggcgtg caggggagga acttcgcctc atcaccgcct atcatcagtc gaccgacgtt
     7501 taccgggtgg aagccatcca cgtctttgtc agggtcaatg gcgttaagga ccttcttttc
     7561 gtcgatatgc ttggggaggg ggagctggac gagaatgcca tgtatcttcg ggtccttatt
     7621 atatttgtcg atgagcttca gcaggtcgac ctcagagatg ctctcgggct gatcgtcctg
     7681 gatcgagaag aaaccaatct catctgcggc cttatgtttg ccggtgacat aactcaccga
     7741 agcaggattc tttcccacca aaatggtgac cagtcccggc acaaccccgt gcttttcctt
     7801 catctcagtc acttgcttct tcagctctgc ccgtatctgg gccgcgatat ccgtaccgct
     7861 aatgattttt gcagacatgt ccttcctcct tggttattta ttttatattg gggccaggga
     7921 tctggagtca gtcacttccc tctcactggt ctctgtcccg gttcctaatc ccttttcttt
     7981 acgagttcca gcagttcctg tcttgtggat tccttgctcc tgaacagccc cctcaccgca
     8041 gaggtgacgg tccttgcccc aggttttttt atccctctca tggacaagca gaggtgttcg
     8101 gcatctatca ccaccatcgc ccccttcggg ttcagctttt tcatgataat gtcggcgagc
     8161 tgagcggtga gccgctcctg gacctggggt ctcttggcga atacctcaag tgcctctgca
     8221 agggcgctca gccccactat cctgccgccc gcgggaatgt aagcaatgtg ggccttgccc
     8281 gcaaacggga gaaggtggtg ctcgcatacg gaataaaaag gaatgtcctt gagaagaacc
     8341 atctcatcat gcttctcccc ctccatgggt ttcaggatat cctccgggga accggcaagc
     8401 cctgaaaata tttcctggta catcttggct actctctccg gtgttctcct gagtcccggc
     8461 ctttctgggt cttcgccgat gccctcaatg atgagcctta cacccttctg tattttcctg
     8521 atatccatga tttaaaaaat taatgcgcat tttagcattt cttatgattt cttgcaaggc
     8581 aaaaaaagac aaaaaccggg atgtgtactt ctcgctaact aacctgcctg cacccctggc
     8641 aatatccctt aatctcaagg gtgtgggtta tccccataaa tctgttggcg ctgcacaatt
     8701 cttcctggat cgcctcaagt tttggagaaa agacctccat cacctttccg catttgatgc
     8761 atatcatatg gtcatggtgt tcatgcccga atatatgctc gtagtgggca tgtttgtcat
     8821 tccttacaac ctgctcaatg agaccgcttt ccacgagaag ggggagcgtt ctgtatatag
     8881 aagccttgga gaccttgatg tgttttcgcc tcatggtggt aaggagttct tcggggttga
     8941 aatgaccgtg acgggagaag acctctgaaa gaattctctg ccgctctttg gtcaacttaa
     9001 ggcctttcct ggaaaggaat tccctgaata tctcctgttc catggagtat cttaaataaa
     9061 aataggagcg ggtttcaatc ctttctgcaa tggatgaccc tttgatctgt cacgatcata
     9121 tggaccctga tatcatgggg tcctgtggga agggaatcca cgagttgctc ctcatatgcg
     9181 agggcgacca atgggattct tcttctcagc cgggacagga gcctgtcgta ataccccccg
     9241 ccatacccga gcctattgcc tttgggatca aaggcagcgc cgggcatgat tacgagcgtt
     9301 acatcgttga tatccctctc tctttcttca gcaaccgcag gctctgggat gcccatataa
     9361 ccggcggaga gttccgagag ttgtttgatt tcataaagcc gcagttcctt gttcagggaa
     9421 tcaacagaag ggagaataac cctctttccg agactgaggg cttcagcaat ctgcgcagag
     9481 gtcgaaacct ctgaacgaaa ggaggagaaa aaaaatagta cgggcgcctg ctgaaattct
     9541 gcaagggaaa agagttgttc ttttatgaga gagtcctttg ccttccttat gtcagggtca
     9601 agggaatctc ttttcctgag gacatcctgc ctgatgcggg atttgccctc caagggtcac
     9661 ccggtgctct gttccaagac ttctttgatt ttccggatgt ctgccacagc accctttatg
     9721 gtggaaggag tcactcctgc aatatcgcat tccatgatct cgtggctaaa gctgataaag
     9781 ccgaggagcg gcgcatcgga cagctgtttc ctgatgaagg caaggtcttc ttctcccctc
     9841 accttgttcg caactataaa gacttttttg accccaagcc cctttgccat ctcctttacc
     9901 gtatgtgctg tctggatgct cctctgacct ggttctacca caacaatgaa ggcatcaacc
     9961 gcctcggtgg ttcccctggt gagatgttct attccggctt ccatgtccac aatcacaatc
    10021 tcgtccctgt tcaccacaag gtgcttgagc agtcttctta gaaacacgtt ttctgggcag
    10081 tagcagccgg acgctgcctc ttttgacttt cctgtcacaa ggagtgtgat gttattaaag
    10141 cggtatccaa actcctcagg gatatcatct accttcggat tgagcttgaa taccccgccg
    10201 cttttccccg gtcgtgcacc cattcgctct tcgatgaggt cggtcatttc cgcaagggga
    10261 cgaatcctgg aaacagcttc aggagggacc cccagcgctg cagccaggtt cgcgtccgga
    10321 tctgcgtcca ccgcaattac cttcttgcct tcagacgcat agagatggct taaaaccgca
    10381 gaaagggtgg tcttgcccac accgcctttt cctgttaccg ctatcttcat ttacgtagta
    10441 taacagaaat gcgcggcgcg catgaccctt cgttgacacc cagtaatttt tcctgatata
    10501 ctggcagcgt aagtgcaaaa tatacaatca aaatttcaaa tcagatggat cccgagaccg
    10561 ttcattttgt gtcagcgaat aatgcgtttc ctggtatgca atctcttggg ggtcaaaaag
    10621 agaacccggg aataagatta tgagtctcgt gggaagactt gaggacctcg cactttctga
    10681 catcttccag attctgagca tcgggaagaa gaccggaacc ctcataatac gcagtgagaa
    10741 gggcaatgcg ttcatcgtat ttaaaaacgg gcttatcgta agagctgaga caaacatgat
    10801 agaagggaat atcggtcatg acctgctaaa aaaaaacgtg gtgaaagagc aggtattcac
    10861 catgggactc gcggtgaaaa agacccttcc ccagaaaccg attgcagaaa tactcttcga
    10921 attggggtcg gtcagtaaag aggccctgga caaggccgca aagaagagga tcgaaaaagt
    10981 ggtatatcat ctcctcctcg cgaaccaggg tgatttccgt tttgaactcg atacccatga
    11041 cgtgggcagt attgctaatg ttgaggacgt gggttgggaa gtttccaagg gaataagtcc
    11101 tgaatacctc ctcatggagg gggcaagggt ccatgatgag atgatgcaga aggagagtct
    11161 ggaggggagc cttgcagagg agacggaagg agaaacatgg gaagaagact gggggaggcg
    11221 cggaaccgtt cccgaaagaa aggatatttc tgcactcaag gcccttaccc aggaactcag
    11281 gttccccaat tccacttcag aaattaccct gctcattctc agatttgcaa gcgatatatt
    11341 ccataggggg gtgctgttta tggtgggaga aactgaactt gcgggactgg gacaatttgg
    11401 attggatatc aagggagcgg acgagaagat acgggagacc gtgctcacct atgcagagag
    11461 tccgtttctc aagggaatta ttgaggcggg tcagtcttat aagggaagaa tggaaaaaga
    11521 tcctgtgacc gaattcctca tgggagatct ggggggggga tggccgatag aggttgcctt
    11581 attccccgtg attacggaag gaaaagtggt tgcgcttttg tactgtgaca atgcggccac
    11641 gggcgaggat ttttccgaga ctgagggact cgaaatattc atcagccagg cgggacttgc
    11701 cctggagaaa tcccttctcc agagaaagtt gcaggagatg caaaaggatg cgggaaagat
    11761 tcctcctgca agcgcgtgat ttcctgtgag aggcatgatt tttttaatgt ccatctgata
    11821 caatgagatt cagcgacaca ctgaggggga tgacgtgaag tcagtactta tcgttgagga
    11881 ttcggctact acaagggcga tgatcagggc ggtgatcgag gatatggagg aagacttcac
    11941 cacactggaa gcgagcaccg gattcgaggc cctcaaattg ctcccccagg ggagttttga
    12001 cctcatcatt accgatatca atatgcctga tgtgaacggc ctcgagctca tccattttgt
    12061 aaagacaaac ccaaactatc aacacatccc catagtgatc gtcaccacag agaggagcag
    12121 cgaggacagg gaaagggggt tggcgcttgg ggccagcgcc tatatcacca aaccgtttac
    12181 ctcagaacaa ctgcaggaag tgatcaccaa aatactcaac ccatgaaatc ttccaagaaa
    12241 gattttatcg tagaggcaga ggaacttatc gaagaagcgg agagactcct tctcgaggtt
    12301 caggaaacct acgccacggg aatcaatccc gacaccataa acgccctctt ccgcaacatt
    12361 catacggtca agggaatttc gggccttttt ggctttaagt atattgccga cttcagccac
    12421 gcctttgaat cactgctgga tgacgtcagg atggggagga tcgagatctc agaggaagtg
    12481 gtgagatttc tcttcagcaa tatcgatctc cttaagcgga tgattgaggg gatcgaagag
    12541 gaaaaggaac aggatgtgtc cgggtatctg aagggcatcg aatcgttcag aaagtcgcta
    12601 aaggggtccg ggggggatca ggaggttcag gagtccctcg ggcagattga ttcatccatc
    12661 ctgaaggtgc tctcggagtt tgaagagcat cgactcaaaa caaatatcaa agaggggaga
    12721 ggtatttatc ttgcgaaagc ggtcttcagt ctcgcggatt ttgatacgtc gctcaatgcg
    12781 ctgacgactc tggtcaagtc gcagggagaa caaatctcta ccctccctac ggccgctgat
    12841 gtgccccccg attccatagg cttcaacatc ctcttcggga gcacaaaacc cctgaaagaa
    12901 ctcacacagg cgatttcttt tcccgtcgat gagctcatca aacacagggc agaaccctcc
    12961 ccttcctctc ctccc
//
LOCUS       DATKOU010000052         9930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4686739, whole genome shotgun sequence.
ACCESSION   DATKOU010000052 DATKOU010000000
VERSION     DATKOU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4686739"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VMU21_02445"
     CDS             complement(<1..682)
                     /locus_tag="VMU21_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HUO76411.1"
                     /translation="MIDLGWFGYLSFDLAFLSSLLSIIFINLVVSGDNAVVIALAVRS
                     LQRNQRRLGIIFGSAAAVLLRVIFTFFAARLLQVPFLRILGGGLIAWIAVKLFVEGAF
                     DDEIHEATNIYQAIKIICIADLVMSVDNVLAVAGASKGNMFLLLFGLGSSIPLVVGTS
                     TLLSILMDRYPIIIYIGAAVLGKVAGEMIITDPLVVKALRPAHALEYAMQGISAVGVI
                     VAGKLLMKR"
     gene            complement(1023..2312)
                     /gene="oxlT"
                     /locus_tag="VMU21_02450"
     CDS             complement(1023..2312)
                     /gene="oxlT"
                     /locus_tag="VMU21_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HUO76412.1"
                     /translation="MEQAKSLDPKVAYGSVVGNRWIQLGMAVLGMVMIANCQYAWTLF
                     VPPLREAFGWSLGAVQLGFTLFIAFETYTMPVEGYLLDRFGPRALFTAAGIMVGIGWT
                     ALGYITSLPGLYFFYALAGTGAAIIYGGSIAMAIRWFKDKRGLAAGIIAAGFGAGSAP
                     FIPFIGWLLKAYGYQTAFIVTGIIQGIIILVVSQLLKYPPGDTGSHAAAKNKADSSRG
                     FNPFEMLRTGQFYLIYLMFCCMTTGGLLITAQTKPYGKDIGIAAWVVIIAVTVDRISN
                     GLGRIGWGGISDKFGRENTMAAVFIANGIFVALLPFLGTGNISFIVLLALIMFTWGPI
                     FALFPSVTADRFGTTYAASNYGVVYTAKGFGGIMGGVVAGYLVTNFGWTTIFMSAGGF
                     AVLAGLGALVLKAIPKPVRIGFQDACPVCELPEQKTK"
     gene            complement(2437..3429)
                     /locus_tag="VMU21_02455"
     CDS             complement(2437..3429)
                     /locus_tag="VMU21_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUO76413.1"
                     /translation="MKAFDYKFQHFQPIKSFKAAPMEEMYVPGHRTCAGCGPALNYRL
                     VAKAAGRDTIFIGPTGCMYVANSSYLCTPYAVSWIHAQITNGGAVASGIEAAYKVLAR
                     KGKYKGPFPNIIVMAGDGGTVDIGLQAASAMMYRGHKILFICYDNESYANTGIQTSPT
                     TPYGANTTFTPPGKMIPEGKTLFPKDPPQLFIGGHPLLKYAATTSVAYPLDLMNKVRK
                     ALSYEGPTFIHMLTPCPKGWKFDEKLTMEMGKLAVETGMWTMYEWENGEYRYTHVPKK
                     YKPVGEYMKHQGRFAHLKEEHIAKMQAFVDEKIKKVKAAAKPVEAPVPTPKKKG"
     gene            complement(3459..4646)
                     /locus_tag="VMU21_02460"
     CDS             complement(3459..4646)
                     /locus_tag="VMU21_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76414.1"
                     /translation="MAQEKMLTGCSAAAQALKLAEVEVICSYPIRPYTAIMMELAKMV
                     ANGELDAEFVHGEGEHAQLSVVLGASACGARAVTGSAGVGVTYAFELYSPVAGGRYPI
                     QMTIADRTLDPPGDFGSEHTDAMSTRDQGWILGWASSPQEVFDNTLMGYRIGEDPRIM
                     LPQMTCQDGYFISHIPDKVKLADLKQVKEFLPPYSAPHPISTTCPVSHGPQIRPDQGA
                     PMDAQRAATFLDLPKVIKEATEDYNRIFGRKYDPFLEKYKLEDAEIAFFIQGAHANTC
                     RKTVDALRRQGLKAGMARPRWVRPWPTHEIAESMGHIKAVGAVETSTSYGGAMRGGNL
                     IHELRASLYDLDERPLTTSFMAGLGGETIWPEDFEFMAKVLSQMIKEKRARKYVYWIG
                     FEE"
     gene            complement(4664..5017)
                     /locus_tag="VMU21_02465"
     CDS             complement(4664..5017)
                     /locus_tag="VMU21_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO76415.1"
                     /translation="MSKKLPEFGTINVPHERAVPFGAVTPAPERQQDLFMTSNWRIVR
                     PVIDHEKCTRCLTCYIACPDSCWAYNEKEDMMEWNASFCKGCQICIKQCPSDALSAVP
                     ELDFEDGVVRLEKPF"
     gene            complement(5061..5699)
                     /locus_tag="VMU21_02470"
     CDS             complement(5061..5699)
                     /locus_tag="VMU21_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HUO76416.1"
                     /translation="MAKVHEITIWTRGVVMDKEGRDIASAFGDAVAMEGKHVQVFDNY
                     EDLPDRVLVTTRKYVRVSDEEIEHKYVYTNDHPDVVVVAEPTIVKGINVLRGMQEGGL
                     LLINTNRPIDYMLKFIPNADLLGAIATVDADGISGVKTVDFSGSEGGVDTAGVGAGIA
                     APIVGAMARVTGMVKKENVAKIVKDVAGMERGYNEVKLQKFRKARAVYSWGG"
     gene            complement(5776..6486)
                     /locus_tag="VMU21_02475"
     CDS             complement(5776..6486)
                     /locus_tag="VMU21_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HUO76417.1"
                     /translation="MLKAVEKKKAYEDIVKQIRDLIEKGRLKKGDQLPTERELTETFK
                     VSRATVREAIRTLESMMLVESRQGNGTYVLASSEEALVQPLAAALFHEKDDLMDIFYI
                     RKLIEPSIAQLAAGNATRDEIKELEEILGKQEQDIENGVSSLETDSAFHTILARMAKN
                     RALGRLLLALVDLLEETREEYLQNGVRARESLQGHQKILAAIRSGDCAAARQAMIRHL
                     EGVEGLLFKKKRGGGKCE"
     gene            complement(6488..8626)
                     /locus_tag="VMU21_02480"
     CDS             complement(6488..8626)
                     /locus_tag="VMU21_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HUO76418.1"
                     /translation="MQDPVMGADLELKVSEAFIEDVGKGWARLDSDDMKALGAALGDL
                     IEISGKQKTIARLTAIADQDRGKRLIQIDGIIRENAQVNVGDKVKVCKVPRKTATSIV
                     LYPLDINSPLPTDAELEYFGKVLQGFPVLQGDKINVPPFGGKDRFFRVEGTSPSGGVI
                     INELTKFVLRQPDYSPESGATVSYEEIGGLERELKLIREMVELPLYHAEVFERLGIEA
                     PKGVLLYGPPGTGKTLIARAIASEAKLYFIRVNGPEIIHKFYGESEARLREIFEEASQ
                     NAPSVIFIDEIDAIAPKRADVLGDVEKRVVAQLLALMDGMVSRGHVIVIGATNIPEMI
                     DAALRRPGRFDREIAIPVPNAEGRLAILKVHSRRMPLSPDVNLERLAQITHGFVGADL
                     EALCKEAGMVTLRRYLSLCKDGDFDFSSAREHLQITMGDFLSGLREIDPTATREFFTE
                     RSTVKWEHVGGLKDIKETLVSTVEWPYRYPELFAAGRVSPPRGILFTGPSGTGKTLMA
                     KALAGETGLNFISISGPILFSKWLGESEKALHELFKKAKQSAPCILFFDEIDALVTRR
                     GISSESGAVERVSSQFFSELDRLSDHSQVIVLGATNREDLIDPALLRAGRLDFILRFP
                     VPDEKGRWEIFMVHASGKPLSEDVDLEELARESEGMVGSDIAFVCKRATMLAIAQVVH
                     ASEGKDSEKLSISAVHFKVALNEVGKKKEI"
     gene            complement(8637..8798)
                     /locus_tag="VMU21_02485"
     CDS             complement(8637..8798)
                     /locus_tag="VMU21_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HUO76419.1"
                     /translation="MAKWKCIACGYIYDPEEGDPDTGIEPGTAFEDLPGDWVCPRCGV
                     TKDMFEKIS"
     gene            9076..>9930
                     /locus_tag="VMU21_02490"
     CDS             9076..>9930
                     /locus_tag="VMU21_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HUO76420.1"
                     /translation="MTPRYNAFGPFLKERFGTIVYKVNVDAGFTCPNRDGTVGVTGCV
                     YCNNDSFRPSACKAEKTITEQVQKGITYLTARYKAKKFLVYFQPYSNTYAPVSELERM
                     YAEALSMPSVIGLAIGTRPDCVDENKIRMLEELAKDRFILIEYGVQSIYDKSLRYILR
                     GHDYQAFLDAIHLTALRGIHIGVHTIMGFPTETRDEMLAMADELSTLPIGFLKIHQLQ
                     VIRDTILAKRYQENPFPTFAYDEYLDFLVEFIQRLSPEIVLQRLFAMAPDDILIAPKW
                     ERTRQEIIR"
BASE COUNT         2270 a         2790 c         2428 g         2442 t
ORIGIN      
        1 tccgcttcat caggagcttt cctgccacaa taacacccac cgcagatatc ccttgcatcg
       61 catattccag ggcatgtgcg ggccggagtg cttttaccac cagcggatca gtgatgatca
      121 tctctcccgc aaccttgccc aataccgcag cacctatata gattattata ggatatctat
      181 ccatgagtat ggagaggagg gtgctcgtgc ccaccaccaa gggaatgctt gagccgagtc
      241 cgaagaggag aaggaacata ttgccctttg acgcgccggc cactgcgagg acattatcaa
      301 cgctcatcac caaatccgca atacagataa tcttgatcgc ctgatagatg ttcgtcgcct
      361 cgtgaatctc atcatcaaac gcgccttcaa caaagagctt caccgcaatc caggcaatta
      421 accccccccc gagaatcctg agaaaaggta cctggagcag tctggcagca aagaatgtga
      481 agattaccct caggagcacc gctgccgctg atccaaaaat gatgcctaac cttctctgat
      541 tcctctgaag agatctgacc gcaagggcaa tgaccaccgc attgtcacct gatacgacga
      601 gattgatgaa gataatgctg agcagtgaag acagaaatgc cagatcaaaa cttaagtacc
      661 caaaccaccc caaatcgatc atccacactc tcctatggaa atgccttata cgtcagcttc
      721 gtgtgcttta caataactgt cctgcacctt ttcactcgga tctcttcatg ccgtggtaac
      781 acagagagat ggcgttcctc accatccttc tcgagacttc agacgaatgc gaggggtgcc
      841 aaagaaagac ccttaacgcc gccgtcctct cccctttttg aaataatccg cctggaaatg
      901 acgtctattg ataatatcac actggtccca gtttctccgt cttctcttga aaaaaaagag
      961 ggggcgttca cagaatgcca cctgtgctcc gcaacccctc aatcccgtgg cgaatgatcc
     1021 tattattttg tcttttgctc cggcagttca catacgggac atgcatcttg aaagcctatg
     1081 cgtaccggct tcggtatcgc cttcaagaca agtgcgccga gccctgccaa cacggcaaat
     1141 cctcctgctg acataaagat cgtggtccat ccaaaatttg taaccagata accggcaacc
     1201 acgccgccca tgatgccgcc gaatcctttc gcggtgtaca cgacgccgta gttgcttgca
     1261 gcatacgtag tcccgaaccg atcagcggtc accgagggga aaagggcaaa tatcgggccc
     1321 caggtgaaca tgatcaaggc gagcagcacg atgaaagata tgttccctgt tccaaggaag
     1381 ggaagcaagg cgacaaagat gccatttgcg atgaacaccg cagccatggt attttcacgt
     1441 ccgaatttgt cggaaatgcc tccccacccg atacggccca ggccgttcga aatgcggtca
     1501 accgtcacgg caataatcac tacccaggcc gcgatgccga tatctttgcc ataaggcttt
     1561 gtctgggccg tgatgagaag accgccggtt gtcatacagc aaaacatgag atagataaga
     1621 taaaactgcc cggtcctgag catttcaaag ggattgaacc ctctgcttga atctgcttta
     1681 tttttagctg ccgcatgaga tccggtgtcg ccgggaggat atttgagaag ctgggaaaca
     1741 acgagaatga ttattccctg aatgatgccc gtcactatga aggccgtctg atagccatag
     1801 gccttcagga gccagccgat gaaggggata aagggcgcgg atcccgcacc gaatcctgct
     1861 gcaataattc ctgcagcaag gcctcttttg tccttaaacc acctgatcgc catcgcaatg
     1921 cttccgccat agataatggc tgctcctgtg cctgcaagtg cgtaaaagaa atagaggccc
     1981 ggcagggacg tgatgtatcc taaagcggtc catccgattc ccaccatgat gcccgcggcg
     2041 gtaaagagtg ccctcggtcc gaaacggtcc aaaaggtatc cctcgaccgg catggtatac
     2101 gtttcaaagg ctataaagag ggtaaagccg agttgtactg ctcccagaga ccagccgaaa
     2161 gcttcccgca aaggcggcac aaataatgtc caggcatact gacagttggc gatcatgacc
     2221 attccaagga ctgccatgcc aagctgtatc cacctgttgc ctactacaga cccgtatgct
     2281 acttttggat ctaagctctt cgcttgttcc atgtcttttc tcccttttgt taatactatt
     2341 tagggatttg cctaacctga gttcttcctc tccctccccg cagggaggga gtagtattct
     2401 ctttccttag ttaggccttt ctgcccatcc tgtcaatcag cccttcttct tgggagtcgg
     2461 gacaggcgcc tctaccggtt ttgccgcagc cttgaccttc ttaatctttt catccacaaa
     2521 ggcctgcatc tttgcgatgt gctcttcctt aagatgagca aaccgcccct ggtgtttcat
     2581 gtattcgccc acaggcttgt acttcttggg aacgtgggtg tatctgtatt caccattttc
     2641 ccactcgtac atggtccaca tacctgtctc gactgcaagc ttgcccattt ccatggtcag
     2701 cttttcgtca aacttccagc ccttggggca cggggtgagc atgtggatga aggtcggacc
     2761 ttcgtaactg agggccttcc tcaccttgtt catcaggtca aggggatatg ccacggaagt
     2821 ggttgctgca tacttcagca gcgggtggcc gccgataaag agctgaggcg gatctttggg
     2881 gaacagggtc ttgccttctg gtatcatctt gccaggcggg gtgaaggtag tattcgcccc
     2941 atagggtgtt gtgggagatg tctggatgcc ggtgtttgca taggactcat tatcgtagca
     3001 gatgaacaga atcttgtgtc cgcgatacat cattgccgaa gcagcctgga ggccgatatc
     3061 aaccgtacct ccgtcacctg ccatgacaat aatattcggg aacggccctt tatatttgcc
     3121 cttgcgcgcg agcaccttat aggcggcttc tatgcctgat gccacggcgc cgccattggt
     3181 gatctgggca tgtatccagg atacggcata gggggtacag agatagctgg aattggcgac
     3241 gtacatacac cctgtaggtc cgatgaatat ggtgtccctg cccgcagcct ttgccacaag
     3301 gcggtagttc agtgcagggc cgcagcctgc gcaggtacgg tgccccggca cgtacatttc
     3361 ttccattggt gcggccttaa atgactttat cggctgaaag tgctggaact tatagtcaaa
     3421 cgctttcatt cattatcctc cttgtaatca gtttaatttt attcttcgaa gcctatccag
     3481 tatacgtact ttcgggccct cttctccttg atcatctgtg agaggacctt tgccatgaat
     3541 tcgaaatcct caggccagat cgtctctccg ccgagaccag ccatgaagga agtcgtaagc
     3601 ggtctctcgt ccagatcgta gagtgaggcg cggagttcat ggatgagatt gccgcccctc
     3661 attgcccctc cgtaggaagt gctggtctct acggctccca ctgccttgat atgccccata
     3721 gattccgcaa tctcatgggt gggccatgga cggacccagc gggggcgtgc cataccggcc
     3781 tttaacccct gtctgcgaag cgcatccacc gttttcctgc aggtattggc gtgggcaccc
     3841 tgaatgaaga atgctatttc agcgtcttcc agcttgtatt tctcgaggaa aggatcatac
     3901 ttccgtccga agatgcggtt gtaatcttca gttgcctctt ttatgacctt gggaagatcc
     3961 aggaaggtgg ccgccctctg ggcatccatg ggcgctccct ggtcaggcct gatctgcgga
     4021 ccatgagaca ctgggcaggt ggtgctgatg ggatggggag cactataggg gggaaggaat
     4081 tccttcacct gcttcaggtc tgcgagtttt accttatcgg gaatatggga gatgaaatac
     4141 ccgtcctggc aggtcatctg gggaagcatg atacgggggt cttcgccaat gcggtagccc
     4201 atcagggtgt tgtcgaatac ctcctgggga ctggaggccc agccgaggat ccagccctga
     4261 tcccgggttg acattgcatc ggtatgctca gagccgaaat cgcccggagg atcgagagta
     4321 cggtcggcaa tcgtcatctg aataggataa cggccgcctg ctacggggct gtacaattca
     4381 aaggcatagg tgactcccac gcctgcgctc cctgtcacgg cgcgtgcacc gcaggcagag
     4441 gcgcccaaca ccacggagag ctgtgcatgc tcgccctcgc catgcacaaa ctccgcgtcc
     4501 agttcaccgt tggcgaccat cttggcaagc tccatcataa tcgcggtata ggggcggatc
     4561 gggtaggagc agatcacttc cacttccgca agctttaacg cctgggcagc tgcagagcag
     4621 ccggttaaca ttttttcctg agccataata ttatctctcc tctctaaaag ggtttttcga
     4681 gacggaccac gccgtcctca aaatcaagtt cgggaactgc gcttaacgca tctgaagggc
     4741 attgcttgat gcagatctga caccccttgc aaaagcttgc gttccattcc atcatgtctt
     4801 ctttttcatt gtatgcccag catgaatcag gacacgcaat gtagcaggtg agacagcgtg
     4861 tgcacttctc gtggtcgatt acaggacgga caatccgcca gttggatgtc atgaagaggt
     4921 cctgttggcg ttccggggcc ggcgttaccg ccccaaaggg tactgctctt tcatgaggga
     4981 cattgatggt cccgaattct ggaagctttt tggacataga atcctccttt tgtctttttg
     5041 tgtacgctcg tagttctttc ttacccgccc cacgagtata ccgcgcgggc tttgcggaac
     5101 ttctgcagtt ttacctcgtt atatcctctt tccatccctg ccacatcctt aacgattttg
     5161 gcgacgtttt ccttcttgac catccctgtc acccttgcca tagcgccgac aatgggggct
     5221 gcgatgccag cgccgacgcc tgcagtgtca acaccgccct cagagccaga gaagtcgacg
     5281 gtcttcacgc cggagatccc gtcggcatcc accgtcgcaa tggcacccag caggtcggca
     5341 ttggggataa acttgagcat ataatcgatc gggcggttgg tattgataag caggagacca
     5401 ccttcctgca tgcctctcag cacattgatc cctttgacta tggtgggttc ggccacaaca
     5461 accacgtcag gatggtcatt ggtgtacaca tacttgtgct ctatctcttc gtcgctgacc
     5521 ctcacatatt tacgggtagt taccagcaca cggtccggca ggtcttcata attgtcgaat
     5581 acctgcacat gtttgccttc cattgcaacc gcatcgccaa aggcgctcgc aatgtcacgg
     5641 ccttctttgt ccatgacaac tccccgcgtc catattgtga tttcgtgaac cttggccata
     5701 cacgtacctc ctcagtattg gccggtgata ccggccggtt taacagttca agaaccccta
     5761 tggttgaata ctacgctatt cgcattttcc accccctctt tttttcttga ataagagccc
     5821 ttctacaccc tccagatggc ggatcatcgc ctgccgtgcc gcagcacaat caccacttct
     5881 tatggcggca agaattttct gatgtccctg gagcgattcc cttgcccgga caccgttttg
     5941 aagatactct tcacgagtct cttccaggag atcaacaagg gcgagaagaa gacgtcccaa
     6001 agcccggttt tttgccattc gcgcaagaat agtatgaaat gcagagtcgg tctcaaggct
     6061 gcttaccccg ttttcaatgt cctgttcctg cttccccaga atctcctcaa gttccttgat
     6121 ttcgtcacgg gtcgcgtttc cggcggcaag ttgcgcgatg gaaggttcta tgagcttgcg
     6181 gatgtaaaag atatccatga ggtcgtcctt ctcatggaaa agagcggcag caagaggctg
     6241 cacaagggcc tcctcactcg aggcgagcac ataggtaccg tttccctgtc tgctctcaac
     6301 cagcatcatg gattccaggg tgcgaatggc ctcacgcacc gtggcgcggg acaccttgaa
     6361 ggtctcggtc agttcccttt cggtggggag ttggtccccc tttttcagtc ttcccttctc
     6421 gatgaggtcg cgaatctgct tcacgatatc ctcatatgcc tttttctttt ccactgcctt
     6481 cagcatgtca gatttccttt ttcttcccca cttcgttcaa tgcaacctta aaatgaaccg
     6541 cggatattga aagcttttcc gaatccttcc cctcagatgc atgaaccacc tgggcgattg
     6601 cgagcatggt cgctctcttg cagacaaagg cgatatccga accaaccatc ccttcagatt
     6661 ctctggccaa ctcctctagg tccacatcct cgctgagggg ttttcccgag gcgtgtacca
     6721 taaaaatctc ccaccgcccc ttttcatcag gcacggggaa cctgaggatg aaatcgaggc
     6781 ggcctgccct gagcagggcg ggatcgatga ggtcctcacg gttggtggct cccaggacga
     6841 tgacctgcga atgatcactc agccggtcca gctcactgaa aaactggctc gacacccgtt
     6901 ccactgcccc gctttcactg gagatacccc tcctggtcac cagggcatct atctcatcaa
     6961 aaaagagtat gcagggtgcc gattgttttg ccttcttgaa gagctcatgg agggcttttt
     7021 ctgattctcc cagccattta gaaaagagaa tggggccgct tatggatatg aaattcaggc
     7081 cggtctcacc tgccagggcc tttgccataa gggtcttgcc ggtcccggag gggccggtaa
     7141 aaagaattcc cctgggaggg gaaaccctgc ccgcagcaaa gagttcgggg tacctgtaag
     7201 gccattctac agtcgagaca agggtctcct tgatgtcctt aaggccgccc acgtgttccc
     7261 acttcaccgt ggatctttcg gtgaaaaact cacgggtggc ggtggggtct atttcacgca
     7321 ggccgctgag gaagtctccc atggtgatct ggagatgctc ccgtgcacta ctgaagtcga
     7381 aatctccatc cttgcaaaga gagaggtatc tccgcaaggt aaccatcccc gcctccttgc
     7441 ataacgcctc caagtctgcc cccacaaacc cgtgggtgat ctgggcgagg cgttccagat
     7501 tcacgtcggg tgagagcggc atgcgcctgc tgtgaacctt cagtatggcg agccgtcctt
     7561 ctgcattagg aacaggaatg gcaatctccc tgtcaaaccg tcccggacgg cgcagcgccg
     7621 catcaatcat ctcagggata ttggtggcgc cgatcacgat cacatgacct ctcgagacca
     7681 taccatccat gagcgcaagg agctgggcca ccaccctttt ctccacatct ccgagaacat
     7741 ctgctcgctt cggagcgatg gcgtctatct cgtcgataaa gatcacgctg ggcgcattct
     7801 gtgatgcctc ttcaaagatt tcccgcagcc tcgcctcgct ctctccatag aacttatgaa
     7861 tgatctcagg accgttgacc ctgatgaaat agagcttcgc ttcgctggca atggcccttg
     7921 cgataagggt tttcccagta ccgggcggac cgtagaggag cacccccttg ggagcctcga
     7981 tccccagacg ttcaaacacc tccgcgtggt acaaggggag ttccaccatc tcacgaataa
     8041 gcttgagctc tctttcaagc cctccgattt cttcgtagga gacagtcgca ccactctccg
     8101 gagaatagtc aggctgcctt aagacaaact tggtaagctc gttgatgatc actccccctg
     8161 aaggtgaagt cccttccact ctgaagaaac ggtccttccc cccaaagggt ggtacgttga
     8221 tcttgtcgcc ctgtagaacg ggaaaaccct gaaggacctt cccgaagtat tccagctcgg
     8281 catcagtggg aagggggcta ttgatatcca aaggatacag gacaatactc gttgctgtct
     8341 tacggggaac tttacatacc ttcactttat cccccacatt gacctgggca ttttccctga
     8401 taatgccgtc tatctggatg agccttttac ccctgtcctg gtcagcgatt gcggtgagcc
     8461 gtgcaatggt tttctgcttg ccggagattt cgatgagatc gcccagtgcg gcgcccaggg
     8521 ccttcatgtc gtccgagtcg agacgggccc atcccttgcc cacgtcttcg atgaatgctt
     8581 cactcacttt taattccaga tccgcaccca ttaccgggtc ctgcatgtcc ttccttctag
     8641 gagattttct cgaacatatc ctttgtaacc ccgcatctgg gacagaccca gtcgccgggc
     8701 aaatcctcaa aagcggtccc cggctcaatg ccggtgtccg gatcgccctc ctcagggtca
     8761 taaatgtagc cgcacgcgat gcatttccat tttgccatgc tcaaaacctc ctcaagttct
     8821 ttgtgcaagt cccttgaacg cgggaattcc cacatgcctc agcgccgacg ttttgtggta
     8881 ttggtctgac catctgatga ccttatgata atatgacctc tgccctttgt caagagcaaa
     8941 aaacagagca aaaataagtc ctggcaaaac taaacctgta gcttaaggtg agagatcaaa
     9001 gggagaaaat cgtcaagaag gatgttcatt cacccttacg ttgacattac cgcttacaaa
     9061 aggaaataat taaacatgac cccaaggtat aatgcatttg gaccctttct caaggagcga
     9121 ttcggcacca ttgtctacaa agtgaatgtg gatgccgggt tcacctgtcc caacagggac
     9181 ggcactgtgg gagtaacggg ctgcgtctac tgtaataacg acagcttcag gccttctgca
     9241 tgcaaggcgg aaaagaccat cacggagcag gtccagaagg gcatcaccta tctcactgcc
     9301 agatacaaag cgaaaaaatt tcttgtgtac tttcagcctt attccaatac gtatgcccct
     9361 gtatcagagc tggaaaggat gtatgctgag gcactttcca tgccttcggt gatcgggctt
     9421 gccataggaa ccagacccga ctgcgttgat gaaaataaga tacgaatgct tgaagagctg
     9481 gcaaaggaca ggtttattct catagaatat ggagttcagt ctatttatga caagtccctc
     9541 aggtatattc tcagggggca tgattatcag gcgtttcttg atgcgatcca tctcaccgca
     9601 ctccgtggca tacacatcgg cgtccacacc attatgggtt ttcccacaga aaccagggat
     9661 gagatgctcg ccatggcaga cgagctgtca actcttccca taggatttct caagatacac
     9721 cagctacagg tcattagaga cacgattctc gcgaagcgat accaggagaa ccccttcccc
     9781 acgttcgcct atgatgaata tctggatttc cttgtggagt tcattcagcg tctttctccc
     9841 gaaattgtcc tccagagact ctttgccatg gctccggatg atattctcat tgctcccaaa
     9901 tgggaaagaa cgcggcaaga aattatcagg
//
LOCUS       DATKOU010000053        12903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4688670, whole genome shotgun sequence.
ACCESSION   DATKOU010000053 DATKOU010000000
VERSION     DATKOU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12903
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4688670"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1591
                     /gene="gspE"
                     /locus_tag="VMU21_02495"
     CDS             62..1591
                     /gene="gspE"
                     /locus_tag="VMU21_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HUO76421.1"
                     /translation="METIEAIRDEDVDSSLLQEVPLSFAKNNLLMPLRKDSGFLKAAV
                     SDDRGLLALLDLAKKYGLTPQPLMAGKGLLIEAINRFYGQVGSAQEVMEGITGEDLSA
                     VATEFESPKDLLELTEEAPIIRLLNALLLQAVKEKASDIHIEPYEKELEVRMRIDGIL
                     HRVLCPPKIIQDALISRMKIMANLDIAEKRLPQDGRIRLLIGARDIDIRVSIVPTFFG
                     ERAVLRLLDRRQGLIGLNEVGLDEKDTKNIEGLLSRTNGIILVTGPTGSGKTTTLYAA
                     LNRIHTEEKNIITVEDPVEYQLKGVGQIHVNPKIGLTFASGLRSILRQDPDVIMVGEI
                     RDYETAEIAIQASLTGHLVLSTLHTNDAASAITRLIDMGVEPFLIASSLVAVVAQRLV
                     RTICPLCKESYTATEEERRYFSVPVSRTSSFTLYRGRGCDRCRGSGYLGRTGIFEVLP
                     MTNEMRQMIAAKKDSQVIKDQAVSAGMRTLYADGLNKAVKGDTTLQEVLRVTQKDYIE
                     G"
     gene            1595..2767
                     /locus_tag="VMU21_02500"
     CDS             1595..2767
                     /locus_tag="VMU21_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUO76422.1"
                     /translation="MPIFKYRGYRTDGTDVDGTIEASGLNDAMVRVREEGIFPSEVVE
                     SGGTKKGIFHRPDQSFLPHMTRQLSLLLSSGVPLMEALQSLSAEHKGVNREMLIAIKE
                     KVSGGVTLYKALEDFPHIFPDFFTSMVHSGEQSGTLDKVLLRLADFLESQNTIKAKVR
                     ASLIYPILMMAVSIVVLSFLFTFVIPKIVKIFKDTKAALPFITVVLIFVSNIFIKYWW
                     IMVAGAVAGTLYVKRFIAGHGLLVDRLILRLPGNILQSLCYSRFARTLGFLLEGGLPM
                     LRALSLAAKSMGNRELEASVRDAERRVAEGQSLSSTLEGFPPVFVQLIATGEKSGRLP
                     ETLKRAADSYEEEFNRKVNKAVSLFEPAMILVMGLVVLFIVLAVLLPMFQLNQLIK"
     gene            2785..3276
                     /gene="gspG"
                     /locus_tag="VMU21_02505"
     CDS             2785..3276
                     /gene="gspG"
                     /locus_tag="VMU21_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HUO76423.1"
                     /translation="MQKVRYNSQDAKLRDLGISDPCREEGFTLLEIIVVVFILSLLAA
                     IVAPRIIGRTDDARIEATKVQIKNFETALKLFKMDNGFYPDTQQGLEALVEKPASGTI
                     PKNYKEGGYLEQKKIPLDPWGNPYVYVSPGLNGDYDILSYGADGKEGGEGKNADIKSW
                     DMK"
     gene            3298..3804
                     /locus_tag="VMU21_02510"
     CDS             3298..3804
                     /locus_tag="VMU21_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUO76424.1"
                     /translation="MPDAGLRDFSLLSRDRLRGHFGKRGGFTLLELMMVVFIISIVLA
                     LSLPTFTGMGDNKLLSDAKRIASILRYLNDSAVSTKESLTLRVDFKDKVIGYNGPEGE
                     KSERIDSLSGVELQSRGMVTEGELLVFFSPLGARENITMHLGDEGSTIAIALNSMNGR
                     VKIIQNEK"
     gene            3844..4239
                     /locus_tag="VMU21_02515"
     CDS             3844..4239
                     /locus_tag="VMU21_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HUO76425.1"
                     /translation="MRVEEPGNAKIRTAHCAHTHAGAGFTLLEVMISLAIVGGLLITL
                     LYTLNYNLGIAERHRVITISTHLAKEKMYEMERKPVGGKGQFPEPYASFSYETNVKDS
                     LFPGMSEITVVVKNAQEEFNLSELVKKAK"
     gene            4236..4892
                     /locus_tag="VMU21_02520"
     CDS             4236..4892
                     /locus_tag="VMU21_02520"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HUO76426.1"
                     /translation="MKSPQLGGAGAFRMGLSTARCGDQGFTLLEVLVALGISAIIMTA
                     LYSAFFLSRKAVDAVDDSLLRLQESRALLDIIKREMESALYESVKPYTLFKLEDRDYY
                     GKQASRVSFTSFSPLIPGLAKIGYSVEEGDGKLVLKKSIGSAFAKTGEPKSIELMEDV
                     ESFTVEARYGDKWVKTWDSAESNSMPEEIRVSVKVYTKKGETPFMVYDVARPRYGKPL
                     "
     gene            4907..5851
                     /gene="gspK"
                     /locus_tag="VMU21_02525"
     CDS             4907..5851
                     /gene="gspK"
                     /locus_tag="VMU21_02525"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HUO76427.1"
                     /translation="MKRSEYSGILNLKCCFFNSEGMVLVLTLMILTLITAMVVEFAYG
                     VYTSTSALYNWRDSQRLSFVAKSGVVMAVNTISGAPQSELYKYLGREIPVPNIPEGFN
                     GSFIIKAEDENGRLNLNSINRTWGDRSVRVYDSFKRLLRNLDLEETIADRVADWIDAD
                     SEPRLRDSEEGAKNAFMDSVDELLLIKGIDQTTYEKLLPYVTVYGFDATDSPLVNINT
                     ASIPVIMSLDERITKAMAEALVNQRVLEPFTMPGELQKVGFSSTLISSISDRITATLP
                     QNVRITSVAEENKIKRIIESTVSINRGNQGTIRYWREM"
     gene            5894..6958
                     /locus_tag="VMU21_02530"
     CDS             5894..6958
                     /locus_tag="VMU21_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL/Epsl periplasmic domain-containing protein"
                     /protein_id="HUO76428.1"
                     /translation="MKTLGFIDLTSRQSGEIYVFRGRTGGYDLEKTVHYTGEGSSQSP
                     DLTGVGEFYLSIPLEMLNFRMMKLPFSEKEKLIKVIPFELESLLMDSADHVVFDAMVM
                     GGGENGFDVLVAYVEKGMLREILTQLSLLSIDPQVVTSLELQAIAKNGTGDVALRLVD
                     PSPLDRDARISAARSELSRHTINLRTGPFAYTRDRERVEKKLKVTFVLLLVLALAINA
                     YLIFGIVSAKTETSSLKKDMRNMYTTLFPNEKRVADELYQMKSHMREIKEKGDALLGV
                     YPLQFLLDLSQKTVPGVAFSEIGLDRDLSTMKGEASSMDDIDKMKTRLSASRANVSVS
                     DIKPLPTGKILFTVVAKAQR"
     gene            6955..7458
                     /locus_tag="VMU21_02535"
     CDS             6955..7458
                     /locus_tag="VMU21_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76429.1"
                     /translation="MTRMFSRSSIAIAIVLLLSILIIPVFMMQRASKKEVDTLKSKRN
                     ELAILTSEFTALKGQVDVVEQKTTSAQVQGVANVLDGIVSSIGIKGKLKSVKGLGSRE
                     IKGPMTEESAEVQLERVSMNELVNMFYRIREAPVILSVKRATMKKAFENPDFLDVTMT
                     IALFTKK"
     gene            7455..8270
                     /gene="gspN"
                     /locus_tag="VMU21_02540"
     CDS             7455..8270
                     /gene="gspN"
                     /locus_tag="VMU21_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HUO76430.1"
                     /translation="MKKAATICVVLVLVIFGLWFVALPESLIISLIDGSLGKENLDFQ
                     TEGFGKGLFYNFNAEKIVLREKGVKGDSYVPLLVFRNVHGWLDFMSLFRIHPELDFEG
                     EMNGGEVAGKVGLTGKENLAIRGNTIRIKGIPFLDFLGIQGDGLLSGDFHMSNDTGEV
                     KFSVADASLKSTSWGGGVVPLEVFHEIKGAATIKNETVEVRSLALSGKGVYGRVRGSI
                     KGMDADMSFELMTDSSFTLGSLTHAVLGQYKVSPGYYVVPLKGKMVKVVEDHK"
     gene            8267..9460
                     /gene="ilvA"
                     /locus_tag="VMU21_02545"
     CDS             8267..9460
                     /gene="ilvA"
                     /locus_tag="VMU21_02545"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HUO76431.1"
                     /translation="MIHIDDIRYAYEHIRPFVHKTPLIFSNSFSKMAGADVYLKAENL
                     QKTGSFKVRGAFNRMFSLKDGKVIAASMGNHAQGVAYAAQRLGVRAKIVMPVTAPIVK
                     EEATRSYGAEVVLYGESFAEALEYAHNQKEYLFIHAFDDDAVIAGQGTVGIEIAEDMK
                     EVDFILVPVGGGGLIAGISTALKAISPKTKVIGVQTESASSASISFRKKGISEKYPSP
                     TLADGIAVGRVGKRTWEIMNHYVDDMIMVREESIAKAILLFLERKKLVVEGAGAVPLA
                     ALMESGERFAGKLVVLVVSGGNIDFTVIDKIIHKGLVSSGRIGVFDVTVEDIPGSLHT
                     LTGIIASDRGNILNVVHDRLAGDLPVGKTRVIFTIETRGEGHLGLILSHLAAKGFEVR
                     ERTKG"
     gene            complement(9462..9959)
                     /locus_tag="VMU21_02550"
     CDS             complement(9462..9959)
                     /locus_tag="VMU21_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HUO76432.1"
                     /translation="MVVAVYSLHNRPIFAALRPDTLSVRVVRVHDGDTVSVMMGRKTE
                     KVRLIGIDAPEMGQRPWGAKAKRYLEGLLSRSGWMVSMEFDIEKRDKYGRLLAYLWTK
                     DNTLLNLEMIQDGYAVLFTFPPNVKHVNLLREGQRLAREKGLAIWGKDGLRETPQDYK
                     RAHPR"
     gene            complement(10257..>12903)
                     /locus_tag="VMU21_02555"
     CDS             complement(10257..>12903)
                     /locus_tag="VMU21_02555"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HUO76433.1"
                     /translation="TPTGSSSSSFFTVIATDKNGCQSGQYYGLSINEINSSLYPAVLP
                     SGTVDNYYSQGFWAGEGGPYTYSIAFGALPPGLTLDPSGVLSGTPTTSGIFDFIVDTD
                     YGDNQYYTLVINASSCLYDTMSVSPTELPSGSEGTLYSSQTFTVNGGTGPYTYSVAFG
                     TLPNGLTLNSSTGDLSGTPIMSGNFPFTLIVTDSSGCKQSSPYYDIQIGCLGITVAPA
                     GLASGTAWISYSQTFTASGGVGPYTYSVTGLLPPGLTLNSSGVLSGMPTAAGTFNFSV
                     GAADSGGCQGSNPYTLVINVGGPPPPLTITATPNPAVAGQFVDIRVSGPFSGTSCPAT
                     ACVDEIAICVSLMLGGTSPLNNAAIWPSMLSTFSTPGSYTLAITPDPSCATAGSTVLV
                     IGCPTITLSPATLPNGTQGTSYSQTITASGGTAPYTYSIATGSLPAGVALSSGGVLSG
                     TPTTSGTFNFTVKATDANGCTGAGAYTWTVAGTPKISISPASPVNFKHVALGKSGVIK
                     LTVSNIGDGDLIISSLAVKKTVDFSQTNDCATVPPQSSCEIEVTFSPNSAGQKKAVLA
                     VNSNDPDTGTRIVYLAGYGTAVSDIVYVTNSGANTVSCIEDGTTSEQVDVGLEPLGIA
                     VSPSGDAVYVTNYDSNTLSVINTATNTVVSTVAVGANPVGVAVSPKGDVAYVANFSSN
                     SVSVIDTSSRQVISTIPVDYGPSGVAVSPDGRILYITNYWSGTVSTIDTLIGMTTASV
                     SVGSGPYGIAVHPSGAVAYTADYGSDTVSFVDTSTRKVIGTTLVGINPTGLALDPSGN
                     FLYVTNLGSNTVSVINTATREVVNTVDAGLRPFGVSVNPQGTLIYVANWGSSSVSIIN
                     AATGTLLETVSVENAPTAFGNFILP"
BASE COUNT         3298 a         3018 c         3532 g         3055 t
ORIGIN      
        1 ccttctctga ttgctgaacg gcagatttct gaacattgca agttcattcc tggactatcg
       61 catggaaacc attgaagcca taagagacga ggatgtggac agttcacttc tccaggaggt
      121 tcccctttcc tttgcaaaaa acaatctcct catgcccctg agaaaggaca gcggcttcct
      181 gaaggccgcg gtctctgatg acagagggct tctcgccctc ctcgacctgg caaagaaata
      241 cgggttgact ccgcagccct tgatggcggg aaaggggctt ctcatagagg ccatcaacag
      301 gttttacgga caggtgggct cggcacagga agtgatggag ggcatcaccg gtgaagacct
      361 ttcggcagtg gccactgagt ttgaatctcc caaggacctc ctggaactta ctgaagaggc
      421 tcccatcata cgtcttctca atgcacttct cctccaggcg gtgaaggaga aggccagtga
      481 tatccatata gaaccctatg agaaggagct tgaggtgagg atgaggatag acggcatcct
      541 ccacagggtc ctgtgtccgc ctaaaatcat ccaggacgcc cttatcagca ggatgaagat
      601 aatggcaaat ctcgatattg cggaaaagcg cctcccccag gacggacgca tcaggctcct
      661 catcggcgca agggatatcg atatcagggt ctcgatcgtg cccacctttt ttggggaaag
      721 ggcggtcctg agattgctcg acaggcggca gggacttatc ggtctgaatg aagtgggcct
      781 cgatgaaaag gacacgaaaa atatcgaagg gctgttatcc aggacaaacg gcataatcct
      841 tgttaccggt cctacgggga gcggtaagac gactacgctt tatgctgcgc tgaacaggat
      901 ccatactgag gagaaaaata tcattacagt ggaagaccct gtggagtatc agctcaaggg
      961 ggttgggcag atccacgtaa atcccaagat agggctcacc tttgcatcag gcctgagatc
     1021 catattgaga caggaccctg atgtgatcat ggtgggtgag atcagagact acgaaaccgc
     1081 ggagatcgcg atccaggcct ccctcaccgg ccatctcgtc ctgagcaccc tccataccaa
     1141 tgacgctgct tctgctatta cgaggctcat tgacatgggg gttgagccct ttctcatcgc
     1201 atcgtcactg gtggcagttg tcgcccagag gctcgtgagg accatatgtc ccctttgtaa
     1261 ggagtcctat acggccactg aagaggaaag gcgctatttt tcggtccccg tctcccgcac
     1321 ctcttctttc accctctacc ggggcagggg gtgtgaccga tgcaggggaa gcgggtacct
     1381 ggggaggact gggatatttg aggtattgcc catgaccaat gagatgaggc agatgatagc
     1441 cgcaaagaag gattcacagg tgataaaaga ccaggctgtc tcggcaggca tgagaaccct
     1501 ctatgcggac ggactgaaca aggcggtgaa aggggacacg acgctccagg aggtgttgag
     1561 ggtcacacag aaagactata ttgaaggatg agaaatgccg atttttaaat acaggggcta
     1621 caggacagac ggcactgatg tagatggcac cattgaggct tcagggctta atgatgccat
     1681 ggtcagggtc agggaagagg gtatcttccc gagcgaagtg gtagagtccg gtggtacaaa
     1741 gaagggaata tttcacaggc cggatcaatc gtttcttccc catatgacgc gtcagctttc
     1801 cctcctcctt tcctcagggg tccctctcat ggaggcactc cagtcccttt ccgctgaaca
     1861 caagggcgtg aacagggaga tgctcatcgc cattaaggaa aaggtttccg gcggtgtaac
     1921 cctttacaag gcactggagg actttcccca tatttttccg gattttttca ccagcatggt
     1981 tcattccggc gagcagagtg gaacccttga taaggtcctc ctgagacttg cggactttct
     2041 cgagagccag aataccataa aggcaaaggt gcgggcttcc ctgatctacc ctatccttat
     2101 gatggcggta agcattgtgg tactttcctt tctgtttacc tttgtgatac ccaagatagt
     2161 aaaaatcttc aaagacacaa aggccgcatt gcccttcatt accgtcgtcc tcatatttgt
     2221 gagcaatatc tttatcaaat attggtggat tatggtcgct ggagcagtcg ccggcacgct
     2281 ctacgtgaag cgttttatcg cagggcatgg actgcttgta gacaggctga tcctcagatt
     2341 gcccgggaat attctgcaga gtctctgtta ttcccggttc gcaaggaccc tggggtttct
     2401 ccttgaaggc ggactcccca tgctgagagc actgagcctc gcagccaaat ccatgggaaa
     2461 cagggagctg gaagcatcgg tgcgggatgc ggagaggagg gtggctgaag ggcagagcct
     2521 ttcttcaacc cttgaagggt ttcccccggt gtttgtgcag ctcatcgcca caggggagaa
     2581 gagcggaaga cttcccgaaa cactgaagag ggcggctgac tcctatgagg aggagttcaa
     2641 caggaaggtg aacaaggcgg tgtccctttt tgaacctgcc atgattctgg tgatgggact
     2701 cgtggtgctc ttcatcgtgc tcgcagttct gctgcccatg tttcagctta atcagctcat
     2761 taaataaacg cggaggcgga ttcaatgcaa aaggtaagat ataactctca agacgcaaaa
     2821 ctaagggatt tgggaatttc agatccctgc agggaggaag ggttcactct ccttgagatc
     2881 atagtcgtgg tctttatcct cagtcttctc gctgccattg ttgcgcccag gattatcggg
     2941 aggaccgatg acgcgcgcat tgaggcaaca aaggttcaga taaagaactt cgagaccgcc
     3001 ctcaagctct ttaagatgga caatggcttc tatcctgata cccaacaggg cctggaagcc
     3061 cttgtagaaa agccggcatc cggcacgata ccaaaaaact ataaagaggg gggctatctt
     3121 gaacagaaga agataccgct tgacccatgg ggcaatcctt acgtgtacgt atctcccggg
     3181 ctcaacgggg actatgatat cctgagctat ggtgctgatg gcaaggaagg gggagagggc
     3241 aagaatgcgg atattaagag ctgggatatg aaatgaaacc agaatcaaga aaaccacatg
     3301 ccagatgcgg gcctaagaga tttcagcctt ttatctcgtg atcggttgag aggacatttc
     3361 ggtaagaggg gtgggtttac cctcctggaa ctcatgatgg tcgttttcat catttccatt
     3421 gtgcttgcac tctcccttcc caccttcacc ggtatgggtg acaataaact attgtcggat
     3481 gcgaaaagga ttgcctccat cctgagatac ctcaatgaca gcgcggtctc cacaaaagaa
     3541 agtctcacgc tcagggtgga ctttaaggac aaagtcatag ggtacaacgg ccctgaagga
     3601 gaaaaatccg agcgaatcga ttccctgtca ggggtggaat tgcaatccag ggggatggtc
     3661 acagaaggtg agcttcttgt ctttttcagc cccctgggcg cacgggaaaa cattaccatg
     3721 catcttgggg acgaaggttc tactatagcc attgcgctga acagtatgaa cggaagggtg
     3781 aagataattc aaaatgaaaa atgagaacgt cattctcaat ccagggataa gaggttgttt
     3841 cgggtgaggg tggaagaacc aggaaatgct aaaattcgta ccgcgcattg tgcacacaca
     3901 cacgccgggg caggtttcac cctccttgag gtgatgataa gcctggccat tgtggggggg
     3961 cttctcatta ctctcctgta cacgcttaac tataacctgg gtattgcgga aaggcatagg
     4021 gtcatcacca tctccaccca tctcgccaag gagaaaatgt acgaaatgga gaggaaaccg
     4081 gtagggggga aggggcagtt tcccgaacca tacgccagtt tttcctatga aacaaatgtc
     4141 aaggattccc ttttccccgg catgtcagag attacagtgg tagtgaagaa tgcacaggaa
     4201 gagttcaacc tcagtgaact cgtgaaaaag gcaaaatgaa gagtccgcaa ttggggggtg
     4261 cgggcgcctt tcgcatgggg ctctcaactg cacgttgcgg tgatcaaggg ttcaccctcc
     4321 tggaggtcct cgttgctctt ggaatatccg ctattattat gaccgccctc tacagtgcat
     4381 tttttttgtc gcgcaaggcc gtggatgcgg tggacgattc ccttctgagg cttcaggaat
     4441 cccgtgccct gctggacatc attaaacggg aaatggagtc cgcattgtat gagagtgtga
     4501 aaccctatac attgttcaaa ctggaggacc gggattacta tggaaagcag gcatcccggg
     4561 tatccttcac ctctttctca cccttgattc cgggactcgc aaagataggc tactcagtgg
     4621 aagagggtga tggaaaactg gtactcaaga agagcatcgg ctcagcattc gctaaaacgg
     4681 gcgagccgaa gagtatcgag ctcatggaag atgtggagtc gttcaccgtg gaagcgcgat
     4741 acggtgacaa gtgggtgaag acctgggaca gtgctgaatc caacagcatg cccgaagaga
     4801 taagggtctc tgtaaaggta tacacgaaga agggagagac tccctttatg gtatatgatg
     4861 tcgcgagacc aagatacgga aaaccgttgt gagcccggac tctggaatga aacgttctga
     4921 atattccgga attctcaatc ttaagtgttg ttttttcaat tccgaaggca tggtgctggt
     4981 tctcacactc atgatactca ccctcatcac cgccatggtg gtggaatttg cctacggggt
     5041 atatacctcc acttcagcac tctataattg gagggactca caaaggcttt cctttgttgc
     5101 gaaatctggt gtcgtaatgg cggtaaacac catttccggt gcgccacagt ctgaactcta
     5161 caagtatctt ggcagggaaa tcccggtgcc caatatacca gagggattca acgggagttt
     5221 tatcatcaag gctgaagacg aaaatgggag gttgaattta aattcaataa atagaacgtg
     5281 gggagataga tctgtgaggg tatacgattc ctttaagaga ctactcagaa atcttgatct
     5341 cgaagaaacc attgcagaca gggttgctga ctggatagac gctgacagtg aacccaggct
     5401 tagagactcg gaagaaggag ccaagaatgc atttatggat agcgtggacg aattactgtt
     5461 gataaaaggg atcgatcaga cgacttatga aaaactattg ccctatgtga cagtttacgg
     5521 ttttgatgca acggattctc ctctcgtaaa tatcaatacc gcctcgatac ctgttataat
     5581 gtcccttgat gaaagaataa caaaagcgat ggcggaagct cttgtgaatc aaagggtcct
     5641 tgaacctttc actatgcctg gcgaacttca aaaggtcgga tttagcagta ccctgatatc
     5701 atcaatcagt gataggatta ccgcaacgct tccccaaaat gttcgcataa catccgtggc
     5761 ggaagaaaat aaaataaaaa ggataattga gagcacagtg agtatcaaca ggggaaatca
     5821 ggggacaata cggtactggc gggagatgtg atttgttgaa ttcggaattg ggcagggtga
     5881 aaaagagcgc acaatgaaga ccctagggtt catcgacctt acctcaaggc agtccgggga
     5941 aatatatgtg ttcagagggc gtaccggggg atacgatctc gaaaaaacag tgcactatac
     6001 cggagagggt tcatcgcagt cccctgatct caccggtgta ggtgaatttt atctcagcat
     6061 tcccctggag atgctgaact tcaggatgat gaagcttccc ttttccgaaa aggaaaagct
     6121 tataaaggta atcccctttg agctcgagag cctgctgatg gacagcgcgg accatgtggt
     6181 cttcgatgcc atggtaatgg gtggcgggga gaatggcttt gatgtgctcg tcgcatatgt
     6241 cgagaaggga atgcttcgag agattctgac acagctatcc ttactcagca tagaccctca
     6301 ggtggtcact tccctcgagc tccaggccat tgcaaagaac gggaccgggg atgttgccct
     6361 gcggctcgtg gatccttccc cattggaccg cgatgcccgg atctcggcag ccaggagcga
     6421 gctgtcacgt cataccatta atctcaggac cggacccttt gcgtacacga gggacaggga
     6481 aagggtggaa aaaaagctga aggtgacatt cgtccttctc ctggtgctgg cgctggcgat
     6541 caatgcgtat ctcattttcg gaatcgtctc agcgaagacc gagacatctt ccctgaagaa
     6601 agatatgagg aacatgtaca ccaccctttt cccgaatgag aaaagggtgg cggatgagct
     6661 ttaccagatg aagtcacata tgcgggagat taaggaaaag ggtgatgccc ttctcggcgt
     6721 gtatcccctc cagttcctgc tcgatctttc ccagaaaact gtcccgggcg tggcattcag
     6781 cgagataggg cttgacaggg acctgagcac catgaaagga gaggcctctt ctatggatga
     6841 cattgataaa atgaagacga ggctctctgc ctcccgggcg aatgtctctg tctcggatat
     6901 aaaaccatta cccacaggta aaatcctttt taccgtggtt gcaaaggcac agagatgacg
     6961 aggatgttct cccgcagctc aatcgctatc gccattgtcc ttctcctctc tatcctcata
     7021 attcctgttt tcatgatgca gagggcctca aaaaaagaag ttgatacgtt gaagtcgaag
     7081 cgcaatgagt tggccattct cacctcagag ttcaccgccc tcaaggggca ggtggacgtg
     7141 gtggagcaaa agactacttc cgcacaggta cagggagttg caaatgtcct ggacggcata
     7201 gtgtcgtcca tcgggataaa gggaaagtta aagtcagtca aaggcctcgg aagcagagag
     7261 ataaaaggtc ccatgaccga ggagagtgcg gaagtgcagc tggagagggt gagcatgaat
     7321 gagctggtga atatgttcta caggataagg gaagctcctg tgatcctttc ggtaaaaaga
     7381 gcaacgatga agaaagcgtt cgagaaccct gattttctgg atgtcaccat gaccattgca
     7441 cttttcacca agaagtgaaa aaggctgcaa ccatctgcgt tgtccttgtc ctggttattt
     7501 tcggcctatg gttcgtcgca ctccccgaga gcctgataat ttcccttata gatggttcat
     7561 taggtaaaga gaacctcgat ttccagaccg aaggtttcgg gaagggactg ttttataatt
     7621 tcaacgcaga gaagatcgtt ttgagggaaa agggggtcaa gggagattct tacgtgcctc
     7681 tcctggtatt ccgtaacgtg cacggatggc ttgatttcat gtctcttttc aggatacatc
     7741 cggaactgga ttttgaggga gaaatgaacg ggggtgaggt tgcgggaaag gtcggattga
     7801 cgggaaagga aaacctggcg atcaggggga acactattcg cataaagggg attcctttcc
     7861 tcgatttcct tggcatacag ggtgatggtc ttctgtcagg agattttcat atgagcaatg
     7921 acacagggga agtgaagttc tcagtagcag atgcgagcct caaaagcaca tcatggggcg
     7981 gcggcgtggt gcccctggag gtgtttcatg agataaaagg cgcagcgacg atcaagaatg
     8041 agacggtgga agtgcgatcc ctcgcccttt ccggcaaggg agtgtatgga agggtcaggg
     8101 gaagcataaa gggaatggat gctgacatgt cttttgaatt gatgaccgat tcctctttta
     8161 ctcttggatc tcttacccac gcggtgctag ggcagtataa agtgtcgcca gggtactacg
     8221 tggtcccttt aaagggcaag atggtaaagg tagtagagga tcataaatga tacacataga
     8281 cgacattcgg tatgcgtacg aacatatcag gccttttgtg cataagacgc cccttatctt
     8341 ttccaactct ttcagcaaga tggcgggtgc ggatgtgtac cttaaggcgg aaaacctcca
     8401 gaagaccggc tcattcaagg tgaggggagc atttaacaga atgttttccc tcaaggacgg
     8461 taaggtgatc gcggcatcca tgggaaacca tgcacagggg gttgcctacg ccgcgcaacg
     8521 actcggtgtg cgtgcgaaga tcgtgatgcc cgtgaccgca cctatcgtaa aagaggaggc
     8581 aacacggagc tacggggctg aggtggtact ctatggagag agctttgcag aggcccttga
     8641 gtatgcgcat aatcagaaag agtatctctt catccatgcc tttgacgatg acgcggtgat
     8701 tgcggggcag gggactgtgg gtatcgaaat cgcggaggac atgaaagagg tggattttat
     8761 tctggtgcct gtgggaggcg gcggtctgat tgcgggaatc tccacggcgc tgaaggccat
     8821 ttcacctaag acaaaggtca tcggtgtgca gactgagtcc gcgtcttcgg ccagtatctc
     8881 cttcagaaaa aagggcatat cagaaaaata tccctcccca acccttgctg acgggattgc
     8941 ggtgggaagg gtcggaaaaa ggacctggga gattatgaat cactacgtgg atgatatgat
     9001 catggtacgg gaagaatcca tcgcaaaggc aattctcctt ttcctcgaga ggaaaaagct
     9061 tgtggtggag ggggccggag cggtccccct tgctgccctg atggaatccg gggagaggtt
     9121 tgcagggaag ctcgtcgtgc tcgtggtgag tggcggcaat atagatttca ccgtcataga
     9181 caaaataatc cacaagggtc tcgtctccag tggcaggata ggtgtctttg atgtcacggt
     9241 ggaagatatc ccgggaagtc tccacacctt gaccggcatc attgcgtctg acagggggaa
     9301 tatcctgaac gtggttcatg acaggcttgc aggggacttg cctgtgggaa agaccagggt
     9361 gatcttcacc attgagacaa ggggagaagg acatttggga cttattcttt cgcaccttgc
     9421 ggcaaagggc tttgaggtga gggagaggac gaaagggtag actaccgcgg gtgggctctc
     9481 ttgtagtctt gaggcgtttc cctcagtcca tcctttcccc agatagcgag gcctttttct
     9541 ctggcaaggc gctggccttc tctcaggaga ttcacatgtt tcacatttgg gggaaaagtg
     9601 aacaggacgg catacccatc ctgtatcatt tccaggttga gcagcgtgtt gtctttggtc
     9661 catagatacg caaggagtct gccgtacttg tccctttttt ctatatcaaa ctccattgac
     9721 accatccatc cactgcggct caacagccct tcgaggtatc tctttgcctt tgcgccccac
     9781 ggcctctggc ccatttcggg tgcgtctatg ccgattaatc tcaccttctc tgtctttctt
     9841 cccatcatga cgcttaccgt atcgccgtcg tggaccctta cgacccttac ggacagggta
     9901 tcgggcctca atgcagcaaa aatcggtctg ttgtggaggg agtaaactgc cacaaccatg
     9961 aaaaaaacga ctgcctaaaa tatcttcata tatcgtctgc tcatgcgtaa ttatatccca
    10021 taccgagttc agaaaatatt ttccgtttaa accttcaggc gatacatcat ttctgatagc
    10081 ttgtgtgtgt atcccgtgat tcttggcaag ctgaaggaaa atggattctt gattgcggga
    10141 caggagtcat cacagagttt tattttagtt tggatgaaag agacggtgta aacatgtcac
    10201 tccagaaggt gatcccccgg cagcatgtgc gcctgcggtt cataacttcc gtccggtcag
    10261 ggaaggatga agtttccaaa agccgtagga gcattctcta cggaaaccgt ttccagcaaa
    10321 gtaccggtag cggcattaat gatcgaaaca ctgctgctcc cccaattggc aacgtagata
    10381 agagtgcctt gagggttgac tgagacccca aatggtcgta agcccgcatc cactgtattg
    10441 acgacctctc gggtggcggt attgatgacc gagacggtat tgctgcccaa gtttgtcaca
    10501 taaagaaaat tccctgaggg gtctaatgcc agccccgtag ggttaatgcc gaccagtgtt
    10561 gttcctatta ccttccgtgt tgaagtatca acaaaggaaa ccgtgtcact cccatagtct
    10621 gccgtatacg cgaccgcacc cgatggatgg accgctatcc cataaggtcc ggaacccacg
    10681 ctgaccgatg ctgtggtcat tccaatcagg gtgtcgatcg tcgaaaccgt gccgctccag
    10741 tagttcgtta tataaaggat ccgcccgtca ggacttaccg ccacacctga aggcccgtaa
    10801 tcaacaggaa tcgtagagat tacctgccgg gaagatgtgt ctatgaccga gacagaattg
    10861 ctactgaagt tggcaacata ggcgacatcc cctttcggac ttactgccac acccacagga
    10921 ttcgctccaa ctgccactgt ggaaactacc gtattggtgg cagtgttaat caccgaaagg
    10981 gtattgctgt cgtagttcgt cacatagaca gcgtcccctg aggggcttac tgctatacca
    11041 aggggctcaa gacccacgtc cacctgctca gaggttgtac catcttcaat acaagaaacc
    11101 gtgttcgcac cggaattcgt gacatacact atgtctgaca ctgcggttcc gtacccggcc
    11161 aaatatacaa tgcgtgtccc cgtatcaggg tcgttggagt tgaccgcaag aaccgccttt
    11221 ttttgcccgg cagaattcgg gctgaaggtg acctctatct cacaggaaga ttgtggaggc
    11281 actgtcgcgc agtcattggt ctggctgaaa tcaacggttt ttttgaccgc tagagaactt
    11341 atgatcaggt ctccatcccc tatgttggat acggttaatt ttattacccc agatttgccc
    11401 agcgccacat gcttaaagtt cactggtgaa gcgggtgaga tcgaaatctt cggggtccct
    11461 gcgactgtcc aggtataagc gcctgcgcct gtgcagccat tggcatcagt cgcttttacc
    11521 gtgaagttga aagtccctga tgttgtgggt gtgcctgaga gcacccctcc agaggaaagc
    11581 gctactcctg caggtaaaga gcctgtcgcg atactgtaag tgtagggtgc tgttccaccg
    11641 cttgccgtga tcgtctgact ataagaggta ccctgtgtgc cgttgggaag cgttgcaggt
    11701 gaaagggtga tggtcggaca accgatcacg aggactgttg agcctgctgt tgcacaggag
    11761 gggtcaggtg ttatggctaa tgtatatgat ccgggggtac taaaagtaga cagcatgctt
    11821 ggccatatcg cggcgttatt cagtggcgat gtccccccaa gcattaagct aacacatata
    11881 gcaatctcat cgacacaggc cgttgccgga caggatgtgc cagaaaaagg tcctgaaacg
    11941 cggatatcaa caaattgtcc ggcaactgca ggattaggtg tggcggtaat cgtgagaggt
    12001 ggcggaggac cgcctacatt gatcacaaga gtataggggt tggagccttg gcagccgccg
    12061 gagtcggcgg cacctacaga aaaattgaat gtgcctgctg ctgtcggcat cccggaaaga
    12121 acacccgagg agttcagtgt cagaccagga ggaagcaacc cggtaacact atacgtatat
    12181 gggcctactc ctccgcttgc cgtaaatgtc tggctgtaag aaatccatgc cgtcccgctc
    12241 gcaagacctg caggtgccac tgttatcccg aggcaaccga tctggatgtc gtagtacgga
    12301 ctgctctgct tgcatccgga agagtcggta acaatcaaag tgaaagggaa attacctgac
    12361 attatcggtg tccccgaaag atcgccagta gaggagttca atgtcagacc gtttggaagt
    12421 gtgccgaatg caacactata cgtatatggc ccggtccccc catttactgt aaatgtctgg
    12481 ctgctgtaaa gggtcccttc gctacctgac ggaagctctg ttggagaaac cgacatcgtg
    12541 tcgtaaagac aactgcttgc atttatcaca agcgtgtagt attggttatc accgtaatca
    12601 gtatctacaa taaagtcgaa tattcctgac gttgtcggtg tgcctgaaag aacacccgag
    12661 gggtcaagtg tcagaccagg aggaagtgcg ccaaatgcaa tactatatgt gtatggaccg
    12721 ccttcaccag cccaaaatcc ctggctgtaa taattgtcca cggttccgct tggaagaact
    12781 gcgggataca aagagctgtt gatttcattt atagaaaggc cgtagtactg gccgctctga
    12841 cagccgttct tatcagtggc aataacggtg aagaaggaag atgaggagct tcctgtgggc
    12901 gta
//
LOCUS       DATKOU010000054         6790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1221891, whole genome shotgun sequence.
ACCESSION   DATKOU010000054 DATKOU010000000
VERSION     DATKOU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221891"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VMU21_02560"
     CDS             <1..678
                     /locus_tag="VMU21_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76434.1"
                     /translation="EIFENYPTLNIIVISGKLNKVEVSEQLFRFTKDNVLKGKRWARH
                     FDVLDKKDDKTDALQRAFSFAVRQKESAGKMRELFLLAESYLEKNMIDKCLEVYQKIQ
                     DLAPGEQESNENIRMFKGEISTEYIEEFYEKGERVAASLLLGHYLEERLKTFTSALLG
                     RSFPALSDCLKALENTHLIGQHHKELFQQLLRLRNKAIHHPAEISKRDLIASIKTLKL
                     LEHAPHQ"
     gene            690..1322
                     /locus_tag="VMU21_02565"
     CDS             690..1322
                     /locus_tag="VMU21_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76435.1"
                     /translation="MKKFILPLGPLLSLTLIGILVLSALLYYRSVKIQRFLEPALAIS
                     QPRMKFTQNISSLITKEFGIGQINGLRFRTGAILVEPSLLLSNIHPAKGSEPLILKKI
                     GSVFLTALNDPEIRENISLILVSTRLPLSPDEKVNKEMRAKMQERAGLILSSLFALEP
                     QLEKTYGAYFAATALSVRAGMKETDWIEFRIVPTERLHIEVLLRLGKYAQ"
     gene            complement(1337..2281)
                     /locus_tag="VMU21_02570"
     CDS             complement(1337..2281)
                     /locus_tag="VMU21_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO76436.1"
                     /translation="MTDTAYDVVIIGGGPAGLSAAQYTARSNLKAVVIDKSPTAGALA
                     YTSKIENYPGLPHALSGTELLDIFRTQAVNFGAEYVEKQVVGVKLEGDLKEVYTMDQV
                     YRGKTVIIATGSMGRKATIKGEAQFLGRGVSYCAVCDAAFYKGMTVCVIGDSEEAVKE
                     AGLLTRFAETVYLISPSKKLKVGDDHLTFALKNLKVLKGLSVTSIEGNEVVERVRMAD
                     SEQNEKVLELSGVFVYLHGSKPVIDFLGNTIELSEEECIVTNKMMQTSIPGVFAAGDV
                     TCAEVRQVVIAASNGCIAALSAEMYIHHRRRHKYDWAK"
     gene            complement(2373..3449)
                     /gene="mqnC"
                     /locus_tag="VMU21_02575"
     CDS             complement(2373..3449)
                     /gene="mqnC"
                     /locus_tag="VMU21_02575"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HUO76437.1"
                     /translation="MKGSRITKRAALSLLKDAELLPLGEMADRIRKEMHPEETVTFVV
                     DRNINYTNVCINKCRFCAFYCDVDSPHAYLLSAEDIFRKIEETIALGGTQILLQGGLH
                     PHLTMDYYLSLLKAIKARYPITIHGFSPPEISYLAAKHDLTIRETLKILKEAGLDSIP
                     GGGAEILSDRVREILSPRKIKSAAWLKVMEEAHYLGMRTTATMMFGSIEKPEDIVDHL
                     DRIRELQERTGGFTAFIPWSFQPGNTELSTAAKAGSGSPGFNVQHPASAVEYLKVLAL
                     SRIYLDNLRNIQASWVTQGLKLAQIALRFGANDFGSTMIEENVVASTGVSYRVSKDDI
                     LHAIRSAGFHPAQRDTCYNVLRRF"
     gene            3603..4178
                     /locus_tag="VMU21_02580"
     CDS             3603..4178
                     /locus_tag="VMU21_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HUO76438.1"
                     /translation="MAKTIEIRWHGRGGQGTVTAAKVLADACLSGGRYVQAFPEYGPE
                     RSGAPLRAYNRVSDNELRLHCPVLHPNVVAVADATLLDGVNVAEGAKEDAIFIVNTSK
                     DPSAIRTKLGLTPTQKICTVDATKIALECFGRALPNAPMLGAVSRATGIIGLDALLQD
                     VKKSFGKKFSQKIIDGNVEATKRGFEEVREG"
     gene            4175..4468
                     /locus_tag="VMU21_02585"
     CDS             4175..4468
                     /locus_tag="VMU21_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO76439.1"
                     /translation="MKPKGWRELPPGSVVTEPGSAARFKTGSWRTFKPEWVPEHCIQC
                     LFCWMFCPDMAIKVKDGKREAFDYDYCKGCGICARECPGKKGQKAIIMVEEGK"
     gene            4469..5647
                     /gene="porA"
                     /locus_tag="VMU21_02590"
     CDS             4469..5647
                     /gene="porA"
                     /locus_tag="VMU21_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HUO76440.1"
                     /translation="MAKVVAVTGNEAIANALRQANPDVCAAYPITPATDIMQRFSGYV
                     ADGLVKTELILVESEHSAMSACVGASAAGGRVITATSSQGLALMWEILFIAAGDRLPI
                     VMPLVNRALSAPINIHADHSDGMGARDTGWVQLWSENAQEAYDNTLQAFRIAEHMDIR
                     LPVMVCLDGFIISHCIERLEYLEDDEARKFIGEYAQLHPLLDVEHPVSYGPLILSDLY
                     MEYKRAQHEVMARVSDVVLNVAAEFEKISGRKYGLFESYRLDDAEIALVILNSAAGTA
                     KDTVDEFRARGVKAGLLKPRLFRPFPYQGVADALKTAKAVCVMDRADSFGGFGPMHME
                     IASALMMNGRKPALINKIYGLGGRDFLPEHAEQVLSELSDIAKGGKIKTFKEYIGVKE
                     "
     gene            5649..6596
                     /locus_tag="VMU21_02595"
     CDS             5649..6596
                     /locus_tag="VMU21_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUO76441.1"
                     /translation="MSTAPSLKELSKKEELFTHGHRMCSGCGAPIVVKQLLLASDYSI
                     VASNATGCLEVSTCISEYTAWKIPWIHNAFENAAATLSGVEAMYRSLKKQGKIAKEIK
                     FVAFGGDGGTYDIGFQSLSGAMERGHDMLYVCYDNGGYMNTGIQRSSATPIGTDTTTS
                     PAGSVLKGKTQQRKNLTRIMAAHGIPYVAQASPAHWRDAVVKFKKALDITGPKFINVI
                     APCNRGWRSSTDDAILLSRIAVDTCYWPLYEVEHGVTKINYTPKEKRPVEEFLRPQGR
                     FKHMFAPGNEWLIALFQDEVDREWERLKREAQGGAQEKF"
BASE COUNT         1681 a         1769 c         1775 g         1565 t
ORIGIN      
        1 gagatattcg aaaactaccc gacactcaac atcatcgtca tcagcggcaa actgaacaaa
       61 gtggaggtat cggaacaatt gttccgtttt acgaaggata atgtgttaaa gggaaaacgg
      121 tgggcaagac actttgatgt cctggacaag aaggacgata agacggacgc cctgcaacgt
      181 gccttttcgt ttgccgtccg ccagaaggaa agcgccggca agatgcggga gctctttctc
      241 cttgccgaat cctatcttga gaagaacatg atcgacaagt gtctggaggt gtatcagaag
      301 atccaggacc tcgctcccgg agaacaggaa tccaatgaaa acatccgcat gtttaagggc
      361 gaaatatcca ccgagtatat cgaggagttt tatgagaagg gagagagggt tgcggcatca
      421 ctcctcctgg gacattatct tgaggagaga ctcaagacat tcaccagcgc gctgctgggg
      481 cgttctttcc cggcgctctc tgattgcctg aaggcactcg aaaacaccca cctcatcggc
      541 cagcaccaca aagaattatt ccagcagctc ttgcgcttaa ggaacaaggc gatccaccat
      601 cctgccgaga tttccaagcg ggaccttatt gcttcgataa aaaccctgaa gttattggag
      661 cacgcacctc accaatgaga gagtaccctg tgaaaaaatt tatcctgccg ctggggcccc
      721 tcttgagtct cacgctcatc ggcatccttg tcttgagcgc actcctctat tatcggtccg
      781 taaaaataca gcggtttctg gaacccgccc ttgcaatctc acagccaagg atgaaattta
      841 cccaaaacat aagctccctg atcacaaagg aattcgggat cgggcaaatc aacggcctca
      901 gattccggac cggggctatt ctggtggaac cgtctcttct gttgagcaac atccatcccg
      961 caaagggatc ggaacctctt atcctgaaaa agatcggaag tgtctttctc actgccctga
     1021 acgatccaga aatcagggaa aatatcagcc tcatcctggt cagtacaaga ctccctctca
     1081 gccctgacga gaaagtgaat aaagagatga gggctaagat gcaggagcgg gctggcttga
     1141 tattaagctc tctgttcgca ttggaaccgc agctcgaaaa aacctatgga gcttacttcg
     1201 ctgctactgc gttgtcagtg agggcgggga tgaaagagac ggactggata gaattccgga
     1261 tcgtgcccac cgagcgcctc cacattgagg tgcttctgag attagggaag tatgcgcaat
     1321 aggccttgag gccgcgctat tttgcccagt cgtacttgtg tctcctccta tggtggatgt
     1381 acatttccgc agaaagcgcg gcgatgcacc cgttcgaggc ggcgatcacc acctgcctca
     1441 cctccgcaca ggtcacatcc cctgccgcaa atacgccggg aatggaggtc tgcatcatct
     1501 tgttggtcac gatacactcc tcttcgctca gttcaatggt attgccaaga aagtcaataa
     1561 cgggcttact gccgtggaga tacacaaaga cgcccgagag ctccagtact ttttcgtttt
     1621 gctcgctatc cgccattcgt accctctcca caacctcatt cccctcaata gaggttacgc
     1681 tcagtccttt taacactttc aggtttttaa gcgcaaacgt caggtgatca tctcccacct
     1741 tgagcttctt tgaaggagaa atgagatata ccgtctcagc aaaccttgtg agcagccctg
     1801 cctccttcac tgcctcctca gagtcaccga tcacgcagac ggtcatcccc ttgtagaagg
     1861 ccgcatcgca tacggcacag tagctcaccc ccctgccgag gaactgggcc tcacccttta
     1921 tcgttgcttt tcttcccatg gaacctgtgg caatgatcac cgtttttccc ctatagacct
     1981 gatccatggt gtagacctct tttaagtcac cttccagctt tactcccact acctgttttt
     2041 ccacatactc tgcaccgaaa ttcaccgcct gtgttctgaa gatatcgagg agttctgtac
     2101 cgcttaacgc gtgaggcaat cccggatagt tctcaatctt gctggtataa gcaagggccc
     2161 ctgcagtagg tgacttatca atcaccaccg ctttcagatt tgaacgcgcg gtatactggg
     2221 cagcgctcag ccccgcagga ccgccaccga tgattaccac atcataggct gtatctgtca
     2281 tatgtcctcc tgtgggaatt cccaacaggc actcgcctta agaattcctg attcccgttg
     2341 cgtggcgtag tacatactat gccgcatccc ggctaaaatc ttctcaagac attatagcag
     2401 gtgtctctct gggcaggatg gaagcctgct gacctgatgg cgtgcagtat gtcatccttg
     2461 gagacccggt agctcacccc tgttgacgca actacgttct cctcaatcat ggtggagcca
     2521 aagtcattgg caccaaaccg cagtgcaatc tgggcaagtt ttagcccctg ggtcacccat
     2581 gacgcctgaa tatttctcag gttgtcaagg tagattcgcg agagtgcaag gaccttcagg
     2641 tactcaactg cacttgccgg atgctgaacg ttgaagccgg gcgatccact gcctgccttc
     2701 gccgccgtac tgagctccgt attccccggc tggaacgacc agggaatgaa tgcagtgaat
     2761 ccccctgtcc tctcctggag ttcccgtatg cgatccaggt ggtcaacaat gtcttcgggc
     2821 ttttcaatgc tcccaaacat catggttgca gtggtgcgca ttccgagata atgcgcctcc
     2881 tccattacct ttaaccacgc agccgattta atcttccttg gactgaggat ttctctcacc
     2941 ctgtcagaga gaatttccgc ccctcctccg ggaatggagt caagccctgc ctctttcagt
     3001 atcttcagtg tctccctgat ggtcaggtca tgtttcgccg caaggtaact tatttcaggc
     3061 ggtgagaaac catgaatggt gatgggatac cgagccttta tcgccttcag cagggagagg
     3121 taatagtcca tggtgaggtg aggatgaagg ccgccctgga gaagtatctg ggtaccgccc
     3181 agcgcgatgg tttcctctat ctttctgaag atatcttcgg ctgacaggag atacgcatgg
     3241 ggactatcga catcgcagta gaaggcgcaa aacctgcatt tatttataca tacgtttgtg
     3301 tagttgatat tcctgtccac cacaaaggtt acggtttcct cagggtgcat ttccttcctg
     3361 attcggtccg ccatctctcc caacgggagg agttctgcat ccttcaataa gcttaatgcc
     3421 gcccttttgg ttatcctgct acccttcaac cgttacctcc atccttcttg ggagtgggga
     3481 tgcgcatctg tgccctgcgt tctattttat aataaacctg tatcggtttt gttataaaat
     3541 cctgttgaaa aagtaggcat gctttagcta aaattttaga cttaactttc cggaggaaca
     3601 gaatggcaaa gacgattgaa atacgctggc acggcagggg gggtcagggg acggttaccg
     3661 ccgcaaaggt ccttgctgac gcctgtttga gcggtggcag gtatgtccag gcttttcccg
     3721 agtacgggcc cgagcgctcg ggggcaccct tgagggctta caatagagtg agtgataatg
     3781 aactccgctt gcattgtccg gtactccatc ccaatgtggt agcagtggcg gatgcaacac
     3841 tcctggatgg cgtgaatgtg gcggaaggtg caaaagagga tgcgatattt atcgtaaaca
     3901 cttcaaagga cccatcggcg attcggacaa agctaggtct caccccaacc caaaagatat
     3961 gtaccgttga cgcgacgaag atagcccttg aatgttttgg cagggccctc cctaatgcgc
     4021 cgatgcttgg cgcagtctcc agggccacgg gcattatagg tctcgacgcc ctcctgcagg
     4081 acgtcaagaa aagcttcggc aagaagtttt cgcagaagat aatagacgga aacgttgaag
     4141 caaccaaaag aggtttcgag gaggtaaggg aaggatgaaa ccaaaaggct ggagagaact
     4201 ccctcccggg tctgtagtga ccgaaccagg gagtgcagca agattcaaga caggctcatg
     4261 gaggacattc aagcctgaat gggtacccga gcactgtatc cagtgcctgt tctgctggat
     4321 gttctgccct gacatggcga taaaggtgaa ggacggcaag agggaagcgt ttgactatga
     4381 ttactgcaag ggctgtggaa tatgtgcgag ggaatgtccg ggcaagaaag ggcaaaaagc
     4441 gatcatcatg gtggaggagg gcaaatagat ggcaaaggta gttgcggtta cgggaaacga
     4501 ggcaatagcc aatgccctca gacaggcaaa tccggatgtg tgtgccgcat atcccattac
     4561 tcctgcgaca gatatcatgc agcggttttc cggctatgtg gctgatggcc tcgtaaagac
     4621 ggaattgatc ctcgtggaga gcgaacacag cgccatgagc gcatgtgtgg gcgcttcagc
     4681 agcaggagga agggttatca ccgccacttc atcccagggc cttgccctga tgtgggagat
     4741 ccttttcatt gctgcgggtg accggcttcc cattgtgatg cctctggtga acagggcgct
     4801 gtcagctcct atcaatatcc acgccgacca ttctgacggc atgggcgcac gggacacggg
     4861 atgggtgcag ctgtggtcag agaatgctca ggaggcctat gacaataccc tgcaggcatt
     4921 cagaatcgcc gagcacatgg atataaggct tcctgtaatg gtctgtctgg acggctttat
     4981 cataagccac tgtatcgaga ggctagagta cctggaagat gatgaggcgc ggaagttcat
     5041 cggtgaatac gcacagctcc acccccttct cgatgtggaa catcccgtga gctacggtcc
     5101 cctcatactc tcggacctct atatggaata caagagggca cagcatgaag tgatggcgag
     5161 ggtgagcgac gtggtcctga atgtcgcggc cgaattcgaa aagatatccg gacggaaata
     5221 tggcctcttt gagtcctacc gccttgacga cgcagagatc gccctcgtca ttctcaattc
     5281 tgctgcaggg accgcaaagg acacggtaga tgagttcagg gccaggggag tcaaggcagg
     5341 gctcctcaaa ccaagactct tcaggccttt tccctaccag ggggtagcag acgccctaaa
     5401 aaccgcgaag gcggtctgcg tgatggaccg cgccgactcc tttggcgggt tcggaccgat
     5461 gcacatggag atcgcctcag cgctcatgat gaacggcagg aagcctgccc tcattaataa
     5521 aatatacgga ctgggcggcc gggattttct cccggaacat gccgaacaag tgctttctga
     5581 acttagtgat atcgcgaagg gtggcaagat aaaaacattc aaggaatata taggagtgaa
     5641 ggaataacat gtcaactgca ccatcgctca aagaactttc gaagaaagag gaactcttta
     5701 cccatggaca caggatgtgt tcgggatgcg gggcgcctat cgttgtaaaa cagctcctgc
     5761 tcgcgtccga ctattccatc gtcgcatcca atgcaacggg atgtctcgag gtctctacct
     5821 gcatctccga gtacactgcg tggaagatcc cctggataca caatgcgttt gagaacgctg
     5881 cagcgaccct gtcgggagtc gaagcgatgt accgctctct caagaagcag gggaagatcg
     5941 caaaagagat aaaattcgtt gcctttggcg gtgacggggg aacctatgat ataggcttcc
     6001 agagcctctc cggcgccatg gaacgcggcc atgacatgct gtatgtctgt tacgacaacg
     6061 gtgggtacat gaatacaggg atccagcgtt ccagtgcgac acccatcgga acagacacca
     6121 ccacctcccc tgcggggagt gtcctgaaag gaaagacgca gcagagaaag aatctgacgc
     6181 ggatcatggc tgcccacggc atcccttatg ttgcccaggc atctcctgct cactggagag
     6241 acgccgtcgt aaagttcaag aaggccttgg atataacggg gccgaaattc atcaatgtca
     6301 ttgctccctg caacagggga tggcgatcca gcactgacga cgctatcctt ctcagcagga
     6361 tcgccgtcga cacctgctac tggcccctct acgaagtgga acacggggtt acgaaaatca
     6421 attatacccc caaagagaag aggcctgtgg aagaattcct cagaccgcag gggagattca
     6481 aacacatgtt tgctcctgga aacgaatggc tcatagccct gtttcaggat gaggtggaca
     6541 gggaatggga acgtttgaag agggaggctc agggaggagc ccaggaaaaa ttctgatcgc
     6601 gccctccgag ggatttttct cagctttttt agtttattgg aaaagccctc aatagtcaag
     6661 ggactggatg gtcttgccat cccccagttt tgacgctggg cgaaatctaa agattcggcg
     6721 atagcaaaaa agatcttgac agatgaaaga ttctcctgta tcttacttgt agtatagcaa
     6781 gtaagaggag
//
LOCUS       DATKOU010000055         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4691150, whole genome shotgun sequence.
ACCESSION   DATKOU010000055 DATKOU010000000
VERSION     DATKOU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4691150"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VMU21_02600"
     CDS             <1..648
                     /locus_tag="VMU21_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HUO76442.1"
                     /translation="NSPRGIRIDASGNVWVANQGDNTITELSSSGALLGTFTVGTGPV
                     DLAIDASGNVWVANQGDSTITELSSSGALLGTFPVGSEPQTVRIDPSGNVWVTNPGND
                     DTPGNTVMKLSPDGTLLGTFTVGSEPHGMAIDSAGNVWITNFGSIAAPGNTVTELSPD
                     GTLLGTFRVGNNPDDIVIDASGNVWVANYGSNSVTVLKGKATGAEFFPYHGPIWP"
     gene            1729..1950
                     /locus_tag="VMU21_02605"
     CDS             1729..1950
                     /locus_tag="VMU21_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76443.1"
                     /translation="MTELGEGREMHWLSKFFPDQKNTAGSPANRTTVLLVIRVIGSLF
                     LVIMALILCFLVTLLVFYLIGLLFSKYFT"
     gene            complement(2241..2927)
                     /locus_tag="VMU21_02610"
     CDS             complement(2241..2927)
                     /locus_tag="VMU21_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76444.1"
                     /translation="MEERRKHRRFIIEGMDIHCKMLFASEVRLLNISFGGAALSLSEK
                     LNMGGEYTLKIESEENVISVPGVVVWEKMTELKKNERGEMVPRYEVGMRFKDVFTYRG
                     DELIDFIESNILEKKFKVRLKGLRVNIVEPEKTTILGYHKDYSVKMISLGGMLLETDL
                     KLEVDNRFHMELAFPEDNEQVQFLGRVAYCSEITGKMPRRYDAGIEFIEMGEEGKKKL
                     NEFIELLQSF"
     gene            3086..3886
                     /gene="cdaA"
                     /locus_tag="VMU21_02615"
     CDS             3086..3886
                     /gene="cdaA"
                     /locus_tag="VMU21_02615"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HUO76445.1"
                     /translation="MELLRQVRWQDILDIVFVAIILYRVLLIIKGTRAAQMLIGLVLL
                     LLASLLSGYLELYTVNWIIQSLWAQIVIVLIVLFQPEIRRALAQMGEARFLSAFTPAE
                     ELKSLDEIVKAAVALANRKMGALIVIERDTSLKDFVEMGTLLDAKVSKEILLSIFHPS
                     SPIHDGAVVIHGNRIIAAGCFLPIALSTEVSKAFGTRHRAGIGLTEETDAIAIIVSEE
                     TGTISMAAAGKLETQIDMENLRNRLTDIFTTTERKKIHRKSEGLKETL"
     gene            3883..>4186
                     /locus_tag="VMU21_02620"
     CDS             3883..>4186
                     /locus_tag="VMU21_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76446.1"
                     /translation="MIKRLFWENLAFKAAAVVLAVILWVFVTSKGQTEMSLSVPIEYV
                     NIPSGLEISKHTVTTAHIVVRTHESLSKNIRQENVRVSVDVGRAKKGEEIFSLKKDD"
BASE COUNT         1061 a         1061 c         1020 g         1044 t
ORIGIN      
        1 aatagtccac gcggcattcg gatagacgca tcgggcaatg tgtgggtcgc aaaccagggc
       61 gacaacacta tcacagagtt gagttccagt ggagcactgc ttggcacatt cacggtgggc
      121 actgggccag tcgacttagc catagacgcc tcgggcaatg tgtgggtcgc aaaccagggc
      181 gacagcacta tcacagagtt gagttccagt ggggcactgc ttggcacgtt tccggttggc
      241 agtgaaccac aaaccgttag gatagaccct tccggcaatg tgtgggtgac aaatccagga
      301 aacgacgaca ctccgggcaa tactgtaatg aagctaagcc ccgatgggac cctgcttggc
      361 acattcaccg ttggcagtga accgcatggc atggctatag actcagcagg caatgtgtgg
      421 ataacgaatt tcggtagtat cgccgctcct ggaaacaccg ttaccgagct aagccctgat
      481 gggaccctgc ttggcacatt cagagtagga aataacccag acgacatagt catagacgca
      541 tcgggcaatg tgtgggtggc aaattacggc agcaacagcg ttaccgtctt gaaaggaaag
      601 gcaacaggag cagaattctt cccgtatcac ggtcctattt ggccataacg aagtttattc
      661 ccccgcagcc cctcttttgg gagcaatctc tgggtgaggc ctcgatcctg aagggtatct
      721 tgcatagtga agactgcagc cgaatcccaa tgaaaagacg gcaatggtag aatcgtcggt
      781 agctcataat gagggagaag tcagaacaca tcccgacaag acatcttttt tttcttgaca
      841 ggcgcctgct aaatgggtgt cggtggaata aacatgaggg gcctggataa gagtagcgct
      901 ttgcagttgg accgcacccc aatccatctg actgcggaat gagccatatt gtcattcctg
      961 ttttgacaaa gatatataga tgcttcaagc tatgcaagta tttatacatc atcgggcacg
     1021 cccttgataa gattatcttc attaatcatc tttaatcaaa acttcattgt catttcataa
     1081 ttgcgttgta acctgagatt gatgcttgca gtgcgtgtct cacttctctc aagggcccct
     1141 tggcgccctc ccgggggcct ctctcttttt agtgttcgct ctgctatctt cattttctct
     1201 tcacattgcc gttgtaagtt agacccgata cgcaatgcgt gtcactttca aagaggcccc
     1261 catcccttcc gggggcctca ccctccttag aaactcaatc atcaatgcag tcctcgcacc
     1321 tcaaaacatg aggggctttg atacccaagc ccccttccac tgtgcagtaa ttgcacacct
     1381 gacaggagca aacaagggat aacaccgtag aagatgctat cccctgaatt gctgttttgt
     1441 cagacaccgt ctgtcattag agaacaagag agaacatcaa ctgggggttt gagtatccct
     1501 cacacggcag gcgtagcagg ttcgaaacct gcatcgccca ccatgttttt ttccgcagta
     1561 tccaccgaag cagaatcccc gtatctgcca ggacatgaaa cccacctagg gcatttaagg
     1621 ggagaagaat cttccctctc tcccagtgct gtgatatgat gcaggtaagt atcatagtcc
     1681 cggtcttggc acctagtaat tcttcattct gcggtaatca agagtgcggt gaccgaactc
     1741 ggagagggac gggagatgca ctggttatcg aaattttttc ctgatcagaa gaacacggca
     1801 ggaagcccgg caaataggac caccgtatta ctggtaatcc gtgtcattgg cagtcttttc
     1861 ctggtcataa tggcattgat tctctgtttt ctggtcactc tccttgtctt ctacctcatc
     1921 gggttgctct tcagcaagta tttcacataa aagataagcc aaagaaggcc ttcgaatgat
     1981 atcctggcat acgaattcac acactcgggg gaggtatccg gcttgaacgg caagactcag
     2041 ggttatgtgc cggtccgcac gagcggaccc agacaaaaag tgggaacaga atgttcttaa
     2101 atgcacagac cccggtatag tcaataaggg ctattgtctt gcggaaagac ggatgcctct
     2161 cctataagaa agacactcat gaataaaatg ggatagaagt gaaacgcgct ttggagggca
     2221 ggatcgcctg ccatggtcaa tcaaaaagac tggagaagtt cgatgaactc atttaatttt
     2281 ttcttcccct cttctcccat ctcaataaac tctattcccg catcatagcg tctcggcatc
     2341 tttcctgtta tttccgaaca ataggcgacc ctcccgagaa actgcacctg ctcgttatct
     2401 tcaggaaagg ccagttccat atggaatcta ttgtccacct ccagcttcag gtccgtctcg
     2461 aggagcattc cccccaggct tatcatcttc acactgtaat ccttgtggta gccaagaatg
     2521 gtggtttttt ccggttcaac gatgttcacc ctgagtcctt tcaagcgcac cttgaacttt
     2581 ttctcgagga tgttggattc gatgaaatca ataagctcat ctcccctgta ggtgaagacg
     2641 tctttaaagc gcatccccac ttcatatcgc gggaccattt ccccccgctc attcttcttg
     2701 agttcagtca ttttttccca caccacgaca ccagggaccg agataacatt ctcttcggac
     2761 tcgattttga gcgtgtattc ccctcccatg ttgagctttt cacttaaact gagtgccgcg
     2821 ccgccaaaac ttatgttcag aagcctgacc tcggaagcaa agagcatctt gcaatggatg
     2881 tccatcccct caatgatgaa tctcctgtgt ttccttctct cctccatggt gaattacccg
     2941 tccactcctt tttacgaagt ctccttccct cgaactgcaa actccttttc agcataccag
     3001 aatcgcggag gaaagagaag ggaaaaccgg cagaaaaaca aaattgtttt gtcaattcag
     3061 cgccagatgc cttataattt atgtcatgga gcttctgaga caggtgcgat ggcaggatat
     3121 cctggatatt gtctttgtgg caataatcct ttaccgtgtc ttattgatca taaaaggcac
     3181 cagggcagcc cagatgctca taggcctcgt ccttttgctc cttgcttccc tcctttcggg
     3241 ctatctggaa ttatacacgg taaactggat catacagagc ctctgggcgc agatcgttat
     3301 tgtgctcatt gtgctgttcc agcccgagat cagaagggcc cttgcgcaaa tgggtgaagc
     3361 gaggttcctc agcgccttta cccccgcgga agagctaaag tccctggatg agatcgtgaa
     3421 agcggcagtc gcccttgcga acagaaagat gggcgcgctc attgtcatag agagggatac
     3481 cagccttaag gattttgtgg aaatggggac gctccttgac gcaaaggtct ccaaggagat
     3541 actcctgagt attttccatc cctcgtcccc catccatgac ggggcggtag taatccatgg
     3601 caacaggata atcgctgcag ggtgtttcct gcccattgca ttaagtaccg aggtgagtaa
     3661 ggcctttggc acgcgccatc gtgcggggat tggtctcacc gaggaaacgg acgccattgc
     3721 gatcattgtg tccgaagaga cagggacgat ctccatggct gcggcgggga aactggaaac
     3781 acaaattgat atggagaacc tgaggaacag gttgaccgac atttttacta ccaccgagcg
     3841 caaaaagatt cacagaaagt ctgagggact aaaggagact ttgtgataaa gaggcttttt
     3901 tgggaaaacc ttgccttcaa ggccgctgcg gtggtccttg cggtgatcct gtgggtcttt
     3961 gtgacatcaa aaggacaaac agagatgtcc ctgagtgtcc ctattgaata tgtaaatatc
     4021 ccctcggggc tcgagatctc aaagcacacc gtgaccactg cgcatatcgt cgtcaggacg
     4081 cacgagagcc tcagcaagaa catacggcag gagaatgtcc gggtctctgt ggatgtggga
     4141 agggcgaaga aaggggaaga gatattctct ctcaagaagg acgatg
//
LOCUS       DATKOU010000056         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2377307, whole genome shotgun sequence.
ACCESSION   DATKOU010000056 DATKOU010000000
VERSION     DATKOU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377307"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VMU21_02625"
     CDS             <1..658
                     /locus_tag="VMU21_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HUO76447.1"
                     /translation="TVSPDESVSDAVSLMKEKKIKHVPVVKDEKLKGIISDRDIKEFT
                     PSKCTTLDIYELHYIFAKTKIKDVMNTKVATTTPEIPVEEAAALMYDQNIGCLPVLEN
                     GKLVGIISDRDIFRVLIGVTGVRHGGIRIYLTIEDKPGTIKDVADVIRNHGYRLQSIL
                     TSYEWVKEGYRRLVIRTKGKGDFKGLRKELEERFKGVSINPGSSADELFTVLKGKSKA
                     "
     gene            complement(689..2089)
                     /locus_tag="VMU21_02630"
     CDS             complement(689..2089)
                     /locus_tag="VMU21_02630"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HUO76448.1"
                     /translation="MPDFRLEKDSLGEIKVPKNAYWGAQTQRAVENFPVSSIRFPEVF
                     IRSLAYIKLACAKVNGDSLLLDKRLSHAIIRACEEIIAGRFNDQFPLDIFQTGSGTST
                     NMNANEVIAARANEMITGKKNSKSPVHPNDHVNMGQSSNDVIPTAIHVSAYIQVKELF
                     IPSMEFLFQTLTHTKKKYKKTVKTGRTHLMDAMPITLEQEMSGWATQVEYSIGRIKDT
                     LPRLAELAIGGTAVGTGVNTQPDFGKRVSKRLSELTNIQFREAKNHFEAQSAQDAIAE
                     LSGQLKTCATSLMKISNDLRWMNSGPMAGLAEIRLPSLQPGSSIMPGKVNPVIPEAMR
                     MVCAQVMGNDTVIAIANASGDFQLNVMLPVIAHSIIQSITILAHASRLLAERAIKGFE
                     VNEGHMTELILRNPIIATVLNPLIGYDKAAEVVKKALRDKKSVKQVVVEMGYLDEKEA
                     DKLLNPAKMTKAGFSE"
     gene            2217..2759
                     /locus_tag="VMU21_02635"
     CDS             2217..2759
                     /locus_tag="VMU21_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO76449.1"
                     /translation="MQESWKFLAKNGEEIMLRYAVPKDAGDIIQTVKSASPERSYVLM
                     EHYGKNPASEKEYITGLNRQRNLLLVALAGSTVVGSLAGLQADGGQRPQTAHILKIGL
                     HLTEAYRGQGIGSEMLNYAIEWAEEHGFKKMEASIFTTNKRSLRMFGRAGFDEECKKR
                     RQFRVGNEFIDEVCMGKLLD"
     gene            2850..>3197
                     /locus_tag="VMU21_02640"
     CDS             2850..>3197
                     /locus_tag="VMU21_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76450.1"
                     /translation="MRKRIFSLTIFSIAMGFLEAVVVVYLRQLYYPEGFVFPLKPMVL
                     EGFYIESLREVSTLIMLVSLGVAAGRILSERLAYILYCFGFWDIFYYGWLKVLLDWPQ
                     SFLTWDILFLIPVV"
BASE COUNT          774 a          721 c          834 g          868 t
ORIGIN      
        1 cactgtcagt cctgatgaga gcgtttcgga tgcagtgagc ctgatgaagg aaaagaagat
       61 aaagcatgtc ccggtagtca aggacgagaa gcttaagggt ataatctcgg accgggacat
      121 aaaggagttt accccttcca agtgcacgac gcttgatatc tacgaacttc actatatttt
      181 tgcaaagaca aagattaagg atgtgatgaa taccaaagtg gcgaccacca caccagagat
      241 tccggtggaa gaagcagcag cgctcatgta cgatcagaat atagggtgtc tgcctgtact
      301 ggagaacggg aagctcgtgg gtatcatttc tgatagggac attttccgtg ttctcatcgg
      361 tgtaaccgga gtaaggcatg gggggatcag gatatacctg accatagagg ataagccagg
      421 cacaataaag gatgtcgctg atgttatcag aaaccacggc tacaggctcc agagtatcct
      481 cacctcgtat gaatgggtaa aggaaggcta cagaaggctc gtgataagga ctaagggcaa
      541 gggagacttt aagggtctca ggaaagagct ggaggagcgg ttcaaggggg tgagtatcaa
      601 tccggggagc agtgctgatg agctctttac ggtactgaaa ggcaagtcaa aggcatagta
      661 agcctgaggt catttcctca gggcttttct attcagaaaa accggccttg gtcatcttgg
      721 caggattcaa cagcttgtct gcctcttttt cgtccaaata ccccatctcc accaccacct
      781 gcttcacgga ctttttgtcc cttaatgcct tcttcaccac ctctgccgct ttgtcatacc
      841 ctatgagggg attcagcaca gtggcaatta tggggttcct caatatcagt tccgtcatat
      901 gcccctcatt cacttcaaat ccctttattg ccctttctgc cagaagccgt gacgcgtgtg
      961 cgagaatggt gatcgactga ataatgctgt gtgcaatgac cggcaacatc acattaagct
     1021 gaaaatcacc tgaggcgttg gctatggcga tgacggtgtc attccccata acctgtgcac
     1081 ataccatccg catagcctca gggatcacag ggttcacctt tcccggcatt atggatgatc
     1141 ctggctggag tgagggaagc cttatctcag ccaggcctgc catggggccg ctgttcatcc
     1201 agcgaaggtc attggagatc ttcatgagac ttgttgcgca ggtctttagc tgacctgaaa
     1261 gctctgcaat ggcatcctgt gcggactgtg cttcaaagtg gtttttggcc tccctgaatt
     1321 gaatatttgt cagttctgac aaccgtttgg aaactctctt cccgaaatcc ggttgcgtat
     1381 ttaccccggt tcccactgca gtaccgccaa tagcgagctc cgcaaggcgg gggagcgtat
     1441 ctttgattct cccgatggaa tactcaacct gagttgccca tcccgacatc tcctgttcca
     1501 gggtgatagg catggcatcc atcagatggg tcctgccagt ctttaccgtt ttcttatatt
     1561 tcttttttgt atgtgttagg gtctgaaaga ggaattccat ggaggggata aaaagctcct
     1621 tgacctggat ataggcggag acgtggattg cggttgggat cacgtcattg gatgactgtc
     1681 ccatgtttac atggtcattg ggatgcaccg gagatttgga attctttttt ccagtgatca
     1741 tttcatttgc ccttgcggcg attacctcgt tggcattcat gttcgttgat gtgcccgagc
     1801 ctgtttgaaa gatgtccaga gggaattggt cattgaacct gcctgcgata atctcctcgc
     1861 atgccctgat tatggcatgg gaaagtctct tatcgaggag cagggaatcc ccattcacct
     1921 ttgcgcaggc gagttttata tacgccaggg agcggataaa cacctcggga aatctaatgc
     1981 tgctcacggg aaagttctcc acagctctct gggtttgcgc tccccaatac gcgttcttcg
     2041 gaacctttat ctctcccagg gaatcttttt ccagtctgaa gtcaggcatg gaaataagta
     2101 tatcattatc cccttccgtt ttctatatct tttgtgagga tgaatagggg ttatattatg
     2161 ttgagggtat tcccgtttag gtgtataatc aaggcaaaga gaggaaggag catacgatgc
     2221 aggagagctg gaaattcttg gccaagaatg gtgaagagat catgcttaga tatgcagtac
     2281 ccaaggatgc gggggatatt attcagacag tgaagtccgc ttctcctgag cggagttatg
     2341 tgctcatgga gcactacggc aagaatccag catcggaaaa ggaatatata accgggctga
     2401 atcgtcaaag gaatcttctc cttgttgccc tcgcgggcag cacagtagtg ggaagccttg
     2461 cgggtcttca ggccgacggt gggcagaggc cgcagaccgc ccatatcctc aagataggac
     2521 tgcatctcac agaagcatac cgaggacagg ggattggttc agagatgtta aactacgcca
     2581 ttgaatgggc agaggaacat gggttcaaga agatggaggc gagtatcttt accacaaaca
     2641 aacgctccct tcgtatgttc ggccgtgccg gattcgacga ggaatgcaaa aaaagaaggc
     2701 agttccgggt cggcaatgaa tttattgatg aggtatgcat ggggaagctg ctcgactagt
     2761 tgcctatctc tgaatggtca tttgttgagt tcaccatgca ctactcgttc ttacctcata
     2821 atctgtaaca gtcgcactct gagactcccg tgagaaagcg catcttctcc ctcaccatat
     2881 tcagtattgc aatggggttc cttgaagccg tggtggttgt gtatctgagg caattgtatt
     2941 accctgaagg gttcgtattt ccgctgaaac ccatggtgct ggagggattt tatatcgagt
     3001 ctcttcgtga ggtttcgact cttatcatgc tcgtctctct gggtgtggct gcagggagga
     3061 ttctttctga gaggcttgca tacatactgt attgttttgg tttctgggat atcttttact
     3121 atggctggtt aaaagtgcta ttggattggc ctcaatcgtt tctcacatgg gatatattat
     3181 ttctcattcc cgtggta
//
LOCUS       DATKOU010000057        11619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_843162, whole genome shotgun sequence.
ACCESSION   DATKOU010000057 DATKOU010000000
VERSION     DATKOU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843162"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VMU21_02645"
     CDS             <1..726
                     /locus_tag="VMU21_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76451.1"
                     /translation="DWVLIISWTVFSAIVISAFFVADRTGFKIRRYALIDYVIKGRLR
                     KFRDANWVVKMSFVVSKMVVPLPLLLCCLFSAGIPGYVSIVAVSASLLIAVCWLLARN
                     HVGFWLRFVLYLTVPILLSLVEAERAPWVSPRMFTFYHLCFGIIALFVLLAVKFTREA
                     KGFRMTTMDFLIIFIAVVVPNLPDKSIRSYNLGMLAVEIITLLFGYEFLITELRGSFG
                     ALTVLTLLSFMLIGVRGGMGLLN"
     gene            759..2753
                     /locus_tag="VMU21_02650"
     CDS             759..2753
                     /locus_tag="VMU21_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76452.1"
                     /translation="MFKRHSISVGIVLVLAVALLAACSGPEQKKVKFYNKGKGLYDKG
                     EYAKAKLEFKNAIQIDVKYADAYYMLGMNALKSGDPGGAHWSFSKVVEISPQRWDAQV
                     QLGKFLLGAGKVDEAMAKAELVLQSEPKNEDALILKGAVMLKKRDYRGARRFLESVVG
                     RDVRGPDGYLLLASIFVQSGDTQNAEKILREGLKLNEKSALLSITLADLALKNKHTDE
                     AADLMQKVIAIEPDVARHRMNLAGIYWDSGKQQQAASVLKAYVSAEPKKEERWLQASA
                     FYISRNRTADAEQEMQEGIRQNKKSFQIRFALSALYLGTNRPDQAVSVLQECLGLEKD
                     PTNPNVIHTKNLLAKFYLSRQETDKAKMYADEVIKESPKNVEANYIEGVIHLDKQEGV
                     QAVSSFRTVISEEPQSIPGHIGLAKAHFINKEPNLAFDTLQKALKIAPDSRDVALTMA
                     GLYAANKDFKSAESQYAKLLAANPNDLGARASLGDLMLRTGNFKRAEEVYTDIKRKAP
                     NNPLGYVKLSALYGAQKKWDRAINEMEQVVRIHPELWSTTNDLAELLNEFGSGKQDLD
                     RALALAQKVISVSPDNPAVLDTLGWIYYRRGETNQAIELLGKAQARNSGNPLINYHLG
                     MAHNRAGNSGKAKEYLQLALNSKLGFRGRDEAEKTMTSIH"
     gene            3306..3860
                     /locus_tag="VMU21_02655"
     CDS             3306..3860
                     /locus_tag="VMU21_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUO76453.1"
                     /translation="MKASEGSERIAPLTALSPATEPGKEGYLIGSGDVLDIAVWKDEA
                     LTRLCVVRPDGFITFPLVGEVRAAGRTASQVKAEIEKRLARYVPEVTLSLEIKQINSM
                     IIYVIGRVNSPGRFILNVDINVLQALASAGGLNPFAKRNSIKILRHGSDETTLYPFEY
                     DEVIEGRHLEQNIFLRRGDVIVVP"
     gene            3881..5233
                     /locus_tag="VMU21_02660"
     CDS             3881..5233
                     /locus_tag="VMU21_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76454.1"
                     /translation="MVIIVLQCYPSLVWGDEFRLVPAASIEQQYNSNILLSTDPLIVT
                     PSGKGDFVTIVSLGADMVERTERLDTDLLVRFDKLIYANHGDLNDTNQTYSGRARYQV
                     TPQLGISCEAAYTKNYNPTLNIIPLPGLPPGLYPIQPFPPLPGEPGGPGGPGGPGTGP
                     GTPSPTAGAFLEAVPWRHITALLAANYQVDETTSATISYSYGNDYYYNNPNYQNDSSQ
                     NVTAVVTHDFSQYLPYLKGWLNLNYNYYWLPDSRNNGISGTVGFSWDFNELWSISSSI
                     GATLQRSELFVTQFIPIPPGLILVARETLNNSGWSGVGGVSLNYKGEYMNGSLTYNKG
                     ITLASGLNGAAVSDSIVLQAGYSLTREWSTALTAAYQTYRSDTSNLNLSTNNVNYQTF
                     FISTGVRYMLPEFLRAGTGRTSLEAFYGYNVNHDSVSHTKADRNLFTVRLHVEYPFFE
                     "
     gene            5334..6836
                     /locus_tag="VMU21_02665"
     CDS             5334..6836
                     /locus_tag="VMU21_02665"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HUO76455.1"
                     /translation="MISLPVVFLISVVVVLIWKPVYRSTATILIEEQEIPPEYVMATV
                     SSYADARLQAINQRIMSAARLLEVINRFNLYADKRKRFTTEEIISNMRGKDVKFETIT
                     ADVVNRRTGQPASATIAFSISYFGENPVVVQEVANVLASLYLEENLKVVSQQTEGATK
                     FLQEEMKDVQAKLAELEKRIAAYKDKNPHAVPELLQFNLNALDGTERNYDQVNDRLPS
                     LREKESYLKTQLATIAPDTKNQDKDLLKELRAKLVMLSSKYSDSYPDVKKTKKEIAEL
                     EERLNAENTNVSVPEQPDNPAYVALASELASVQSEIESAKRQLEDLDKRRNEYRRRVE
                     LFPKVEEGYKNLMVERNSTQAKYDDLMKKFMEAKVAHGLEKEKMGERFTLIDPARLPE
                     KPVRPNRPLILIMGLILGIGAGAGTASLREATDRSARGAEDLATVIPLPILAEIPEIV
                     SAEDALCRRRRLRMAVGITVLLVVGLVLGIHLFVMDMDLLWVRMMKYLNL"
     gene            6870..8144
                     /locus_tag="VMU21_02670"
     CDS             6870..8144
                     /locus_tag="VMU21_02670"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO76456.1"
                     /translation="MDKIKTTISSLKYKLSGGKKKTPDASLRSTPEKEEDEQYRAKME
                     KAVSFFKQRQGGKKETPEVPLENTPDVTPQGEAEVRRKVTTAVSLQDDNGQKDGMHQE
                     EDSGGMKETPEASIRSAPEKQDNGRYRDKIGKAMSFFKQRQGGKGETPEVFSENNPEV
                     MPQSAAEVRAEVTTAVPLQDSDGQKDSMRRERDNERIGWISPTYSQSRRVHLDPRFVS
                     RNRCLAYLDNVPGAEAYRMLRTQLLQRTWGTGNNTIMVTSALPGEGKTLTAINLAFTV
                     AREFRNTVLLVDGDLRKQSIHKYLGCPGGKGLVDYLVDGSPVSELITWPGIEKITLIS
                     GERSFQESAEILGSQRMKELVFDMKQRYPERYIIFDVPPILTGADTLTFVSLVDLVIV
                     VVQAGKTSKEDVKKALQFLPKGKVFGLVLNRC"
     gene            8470..9024
                     /locus_tag="VMU21_02675"
     CDS             8470..9024
                     /locus_tag="VMU21_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HUO76457.1"
                     /translation="MAKLELKGAVRGKVRKKTSEPLVPLEETAPKGLKRLAKARTSNL
                     VASVEMMGRNEKCYGLREERCSKETVNLGMPQEISAGRACGIKKHYLTSNVSCWLTFR
                     LPKEAVGLAKKVAIVGDFNKWDKEASPMKKLRSGDFEITLELSTGREYRFRYLMDGHR
                     WENDWCADRYERNPYGGDDSVVIV"
     gene            complement(9193..9879)
                     /locus_tag="VMU21_02680"
     CDS             complement(9193..9879)
                     /locus_tag="VMU21_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76458.1"
                     /translation="MFTLIDRKRTKDDWFAYDAEIRSIFKEMAIDLVIYLGLNDGQLA
                     PKLRAFYTGEILSFRPVSSVFERLAVTAFSGRNWHIHKCSSGRQGPNQTINVYDETVY
                     SPLRKAGEYCTKCSGEGTIATEEKVSVQSLGEVLGEIVPGIESKHIFLKTEIQANDTE
                     VLKGLGNKLECVMAVQTKLSLVPTYERMPHWTESISAYERNGFGVLGINPSNRDSGRI
                     NEYDCLLMRV"
     regulatory      10976..11050
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11247..>11619
                     /locus_tag="VMU21_02685"
     CDS             11247..>11619
                     /locus_tag="VMU21_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76459.1"
                     /translation="MEAEVVTARTMLGKKKGEEKMRKLIAAACMALLFTAGTAKADIA
                     YPGAIAYWNQYGQTGQQVSTPGTGSSNITAIDMTRGVGLLTDSAGANSLNSNGWTGEA
                     TDYIQFGLTIASGYEANLNQLL"
BASE COUNT         3108 a         2620 c         3052 g         2839 t
ORIGIN      
        1 gactgggtgc ttattatttc ctggacagta ttttctgcaa tcgtaatcag cgcctttttt
       61 gtcgcggaca gaaccggctt caagataaga cgctatgcac tgatcgatta tgtcattaag
      121 ggcaggctaa ggaaattcag ggatgccaac tgggtcgtca agatgtcctt cgttgtgtcg
      181 aagatggttg ttccgctgcc tcttctgctc tgctgtctct tctctgcggg catccccgga
      241 tatgtctcta tcgttgccgt ttccgccagc ctgctaatcg cagtgtgttg gctattggca
      301 aggaaccatg tgggcttttg gctccgcttt gtcctttatc tgaccgtccc cattcttctc
      361 agtcttgttg aggcggaaag ggcgccctgg gtcagcccaa gaatgttcac gttttaccac
      421 ctctgtttcg gtattatcgc attgtttgtg ctgcttgccg taaagtttac ccgcgaagcg
      481 aaaggattcc ggatgacgac catggacttt ctgatcattt ttatcgcggt ggtcgtgcct
      541 aacctgcctg ataagtcaat caggagctat aatttgggca tgctcgcagt ggagataatc
      601 actcttttgt tcggctacga atttctgatt acagagctcc ggggaagctt cggcgccttg
      661 acggttttga ccctgctgtc ctttatgctc attggtgtgc ggggtggtat gggactgttg
      721 aactgaaaga actatgaaat ggagaaggag gactcacgat gttcaaaagg catagtatat
      781 ccgtaggcat tgtgcttgtg ctggctgttg ccttgttggc ggcttgcagc gggcctgaac
      841 aaaaaaaggt taagttttat aacaagggca agggcttgta tgacaaaggg gaatatgcga
      901 aggcgaagct ggagttcaag aacgccatcc agatagatgt gaaatatgcc gacgcatact
      961 atatgcttgg catgaatgca ctaaaatcag gagaccccgg tggagcccac tggagcttct
     1021 ccaaagtcgt agagatctca ccgcagcgtt gggatgcaca ggttcagctg ggcaagtttc
     1081 tcctgggcgc tggcaaagtg gatgaggcaa tggctaaggc ggaacttgtc ctgcaaagcg
     1141 aaccaaagaa tgaggatgcg cttatcctca agggcgctgt catgctgaag aagagggatt
     1201 acagaggcgc gcgccgcttc cttgaatctg ttgtggggcg cgacgtgcgc ggacctgacg
     1261 gttacctgct tttggcctct atctttgtgc aaagcggaga tacgcagaat gccgagaaaa
     1321 ttctgcgtga gggccttaag ttgaatgaga agtcggcgtt gctctccatc acccttgccg
     1381 accttgccct gaaaaacaaa catactgatg aggcagccga ccttatgcaa aaagtgattg
     1441 cgatagagcc tgatgttgcc cgtcatcgca tgaacctggc ggggatttac tgggattcgg
     1501 gcaaacagca acaggcggcg agtgttctca aggcctatgt atcagcagaa cccaagaaag
     1561 aggaacgttg gcttcaggcg tccgctttct atatctcaag gaacagaaca gctgatgctg
     1621 agcaggaaat gcaggaagga atacgccaga ataaaaagag ctttcagata cgttttgcgt
     1681 tgagcgctct ctatttgggc acgaaccggc ccgatcaagc ggtttccgtc cttcaggagt
     1741 gtctcggtct ggagaaggac ccgacgaatc cgaatgttat ccatacaaag aatcttctgg
     1801 cgaaattcta tctttcgcgg caggagaccg acaaggcgaa gatgtatgca gatgaggtca
     1861 taaaagagag tccgaaaaac gtggaagcca attacatcga gggagttatt catctcgata
     1921 agcaagaagg ggtacaggct gtttcttcat ttcgcaccgt tatcagtgag gagcctcaat
     1981 ccatacctgg ccatatcggt cttgcgaagg cacatttcat caacaaagag ccgaatctcg
     2041 ccttcgatac cctccagaag gccttgaaga ttgcacctga ttcaagggac gtagctttaa
     2101 caatggcggg tctctatgcg gcgaataagg atttcaagag cgcggaatcc cagtatgcca
     2161 aactcctggc cgcgaaccca aatgacctgg gagccagggc cagcctgggt gatcttatgc
     2221 tgagaacagg caactttaag cgggcagagg aagtgtacac tgatataaag aggaaggccc
     2281 cgaataaccc cctggggtac gtaaagctga gtgctctgta tggcgcccag aagaaatggg
     2341 acagggcaat caacgaaatg gagcaagtgg tccggataca tcctgaactt tggtcgacaa
     2401 cgaatgacct tgccgagttg ctgaacgaat tcggcagcgg gaagcaagac ctggacaggg
     2461 cactggcgct ggcccagaaa gtcatatccg tcagtcctga caaccctgcc gttttagaca
     2521 ctctcggctg gatctattat cgcaggggtg aaacaaatca ggccattgaa ttgctgggaa
     2581 aggcacaggc caggaattcc ggaaatccct taatcaatta ccatctgggg atggcccata
     2641 atcgcgcagg caattccgga aaagcaaagg aatatctcca gcttgccctg aattccaaac
     2701 ttggtttccg cggcagggat gaggccgaaa agacaatgac cagcatccat taacacataa
     2761 atcccccggt actgtaaaag aggcacgagc gacacaaggg atggcttgcc tcatgctgaa
     2821 tagattttgc cctcatttat gagcgcattg gctatatatt catatacgcg tctttaaact
     2881 aaacttaatt tctctgtaac aaaattgtaa ccaaggtgta acaattattg tagtaaaatt
     2941 agtgattatc cctcgtggca agctgcgggg tccctattgc acaacatgat ggcgttgtgg
     3001 aggccttgga agattgggga gtccttgcgc ctccttaatg accctatacg caagacatgc
     3061 tttcatccct gtaaagacac ggggcatttt gcaaagaggg gttcgtaata aaaaggattc
     3121 tattcatgat gcttgataga gttttttcta caacgtcttg attttcgata caaaacaatg
     3181 gtcaaaatgt ggcggaggga taatacatgt ttcgaatgat tttgattctt tttatggcgg
     3241 ccggggtgct tcttgcttat tctgtgtcct tgtcacgggc tggggaagga gatgctgcgc
     3301 ctcgcatgaa agcttcagag gggtccgagc ggattgcgcc tttgacggct ttatcgcccg
     3361 cgacagagcc tggcaaagag ggttacctta tcggatctgg cgatgtgctg gatattgccg
     3421 tatggaagga tgaggctttg actcggttat gtgttgtccg gcccgacggg tttatcacat
     3481 ttcctctggt cggggaggtc cgggctgccg gaagaacagc atcacaggtg aaagcggaga
     3541 tagagaaaag acttgcccgc tatgtaccgg aagtaacgct ttctctggag ataaagcaga
     3601 tcaacagcat gattatttac gtaataggca gggtgaatag tcccggccga tttatactaa
     3661 acgtcgatat caacgtcctt caggccctcg catcggccgg agggctcaat ccttttgcaa
     3721 agaggaatag tatcaagatt ctcaggcatg gaagcgatga aaccacccta tatccattcg
     3781 agtacgacga ggtcatcgag ggaaggcatc tggaacagaa catctttctc agacggggcg
     3841 atgtcatcgt cgttccctga tgaattgcag gcgctggctg atggttatta ttgtgctgca
     3901 gtgttatcca tcgcttgtgt ggggggacga attccgtctt gtgccagctg cctcaatcga
     3961 gcagcaatac aatagcaaca tcctgctttc gactgaccct ttgatcgtga cgccttctgg
     4021 aaagggagat ttcgtcacca tcgtctcgtt gggcgctgat atggtagaac gcacggagcg
     4081 actcgataca gacctgctgg ttcggtttga caagcttata tatgccaatc acggggacct
     4141 caatgatacg aatcagacat acagcgggag ggcccggtat caagtaacgc cccagcttgg
     4201 tatctcatgc gaggctgcct acacaaaaaa ctataacccc acactgaaca taatcccgtt
     4261 gcctgggcta ccacctggac tttaccctat tcagcctttt cccccattac ctggggagcc
     4321 cggcgggcct ggcgggcctg gcgggcctgg tacgggaccg ggaactccct ctccgacggc
     4381 tggcgccttt ttggaagctg taccctggag gcacatcacc gctttattgg ctgctaacta
     4441 tcaagttgac gaaacaacat cagccacgat atcctacagc tacggcaacg actattacta
     4501 taataacccg aattatcaga acgattcatc ccagaatgtt accgcggtgg tgacacatga
     4561 ttttagtcag taccttccct atctgaaagg atggttgaac ctgaactaca actattattg
     4621 gcttcccgat tccaggaata atggtatctc gggaaccgta ggcttttcat gggatttcaa
     4681 tgagctctgg agcatctcca gctcaatcgg tgccacgctt caaaggtcgg aactatttgt
     4741 gacacaattc attcccatac ctccaggtct catactcgtg gcaagggaaa cgttgaacaa
     4801 ctccggctgg agcggagtgg gaggggtctc ccttaattac aagggcgagt acatgaatgg
     4861 cagcctcacc tacaataagg gtattacact agcctctggt ctgaatggcg cggcagtgag
     4921 cgacagcata gtactccaag ctggttattc actcacgcgt gagtggtcga ctgctctgac
     4981 agccgcctat cagacataca ggtctgatac ttcaaattta aatctgtcga ctaataatgt
     5041 aaactatcag acattcttca tcagtacggg ggtgcgttac atgcttccgg aatttctccg
     5101 ggcagggaca ggacgcactt ccctggaggc gttttacggg tataacgtga atcacgatag
     5161 cgtgtcgcac acgaaggcag ataggaactt gttcactgtc cggctgcatg ttgagtaccc
     5221 tttttttgaa taggggtaaa gcgaaataag agaaaggacc tttgatggag caagagaaga
     5281 cattgcgcgc tgaagatatc ttgcgaattg cctggcggcg acgttgggga ttcatgatat
     5341 cgttgcctgt cgttttcctc atttcggttg tcgttgtcct gatatggaag cctgtgtatc
     5401 gctccaccgc cacgatcctc atcgaagaac aggagatccc gccggaatat gtgatggcaa
     5461 cggtatcaag ttatgcggac gcgagactcc aggccatcaa tcagaggatt atgagcgcag
     5521 cgcgacttct cgaagtcatc aatcgtttca acctttacgc agataaaagg aagaggttta
     5581 ccaccgagga gatcatctcg aatatgcgtg gaaaagatgt gaagttcgag accattacgg
     5641 ctgatgtggt aaatcgcagg acaggtcagc ctgcttcagc gactattgca tttagcatct
     5701 catatttcgg ggaaaatcct gtggtggttc aagaggtcgc taatgtcctc gcctctttat
     5761 atctggagga gaacttgaag gtcgtaagcc agcaaactga gggcgccacg aaattccttc
     5821 aagaggaaat gaaggatgtc caggccaagc tggcagagtt agagaaaaga atcgctgcct
     5881 ataaggacaa aaacccccat gccgttccgg agctccttca gttcaacctt aacgccctgg
     5941 atgggaccga gaggaattat gaccaggtga acgaccgtct cccctctctt cgcgagaagg
     6001 agagctatct gaagacccaa ttggctacta ttgcgcctga cacgaaaaat caggataagg
     6061 acctcctgaa agaactcagg gcgaagctcg tgatgctaag ctcaaaatac tcagactcat
     6121 atcccgatgt gaaaaaaacg aaaaaggaaa tcgctgagct tgaagaaagg cttaatgctg
     6181 aaaacactaa cgtgtccgtt cctgaacaac cggacaaccc tgcatacgtt gcgcttgcct
     6241 cggagttagc cagcgtacag tcagagatcg agtctgccaa gagacagctg gaggatctcg
     6301 ataagaggag aaatgaatac cggcgaagag tggaactgtt ccccaaagta gaggaaggct
     6361 acaagaacct tatggtcgaa agaaacagta cccaggcaaa atacgatgat cttatgaaaa
     6421 aattcatgga agcgaaggtg gcacacggcc ttgaaaagga aaagatggga gagcgtttta
     6481 ccttgatcga tcctgcgagg cttccggaga aaccggtcag gcctaaccga ccccttattc
     6541 ttatcatggg gcttattctc ggtatcggag cgggcgccgg gacggcgtcg ctgcgagagg
     6601 ctacagaccg ttcagcccgc ggggcagaag atctggcgac agttattcct ttgccaattc
     6661 tcgcagagat accggagatt gtctcagcgg aggatgcgct ctgcaggaga agacgcttga
     6721 ggatggccgt cggaataaca gttctcttag tcgttggtct cgtgctgggt atccatttgt
     6781 ttgtgatgga tatggatctc ctctgggtac gtatgatgaa gtatttaaat ttataacaag
     6841 gaaacagact gggaaggtgt gattcgaacg tggacaagat aaaaacaacg atatcttctt
     6901 taaagtataa gctaagcggc ggcaagaaaa aaacgccaga cgcatccttg cgtagtactc
     6961 ctgagaaaga ggaggatgag cagtacaggg ccaagatgga aaaagctgta tcatttttca
     7021 aacaaaggca aggcggcaag aaggaaacac cggaggtccc tttagaaaat accccggacg
     7081 taacgcccca gggagaggct gaggtgagga ggaaggtgac aactgctgtc tctttacaag
     7141 atgataatgg acagaaagat ggtatgcatc aggaggaaga tagtggcggc atgaaagaaa
     7201 caccggaggc gtccatacgt agtgctcctg agaaacagga taatgggcgg tacagggaca
     7261 agatagggaa agcaatgtca tttttcaagc aaagacaagg cggtaagggg gagacgcccg
     7321 aggtcttttc agaaaacaac cctgaggtaa tgcctcagtc agcggctgag gtgagggcag
     7381 aggtgacaac tgccgtacct ttgcaagaca gcgatggaca gaaagacagt atgcgtaggg
     7441 agagagataa tgagagaatc ggatggatat ctccgactta ttcacaatcc cggagagttc
     7501 atttagatcc gcgattcgtc tcgagaaaca ggtgtttggc ttatctggac aatgttccag
     7561 gagcggaggc ttatagaatg ctacggacac aattgttgca gcgaacttgg ggcaccggga
     7621 acaataccat tatggttacc agtgctttgc cgggcgaagg taagaccctc acagccatca
     7681 atcttgcctt caccgttgcc cgtgagttcc ggaatacggt cctgctggtt gatggagacc
     7741 tgagaaagca gagcattcat aaatacctgg gatgccctgg tggaaaggga ttggtagatt
     7801 atcttgttga cggttcgcct gtttccgaat taatcacctg gccgggcatc gagaagatta
     7861 cacttatttc cggagaacgt tcttttcagg aaagcgccga gattcttgga tcgcagcgta
     7921 tgaaagaact tgtgttcgac atgaaacagc gctatcctga gcgatacatt atttttgatg
     7981 tgccccctat tcttaccggt gctgacacct taacgtttgt ttctctcgtt gacctggtga
     8041 ttgttgttgt tcaagcgggc aaaacctcga aagaagatgt taagaaggcg cttcagtttc
     8101 ttcctaaggg gaaggttttt gggcttgtct tgaaccgctg ttagtcaccg tgcttggtag
     8161 aatacgtgac ctgcgttgct gttctgtaca gatgtgtcgg cttcgctaag tattccacag
     8221 aattcctgaa atggctctgg tgagcagcaa gaaaggctac aatgtgaggt tcgcctccat
     8281 actttgaaat ccgtgttctt tgaaaaagaa caatcccgac tctcaacaat aatttaacag
     8341 ttatttcatg agccggacat gtttctgtaa taatagagcg ctaatctaat agcactgtcg
     8401 gcggatgcct tcagatgaaa gggagttaat catgagggag aaaaaagaaa cgtttgagga
     8461 ggcagcgata tggcgaaact tgaattaaag ggagcggtga ggggaaaagt gaggaagaaa
     8521 acatctgagc ctttagtccc tctggaggaa acagccccga aaggactgaa aaggctcgct
     8581 aaagcaagga catccaatct tgtggcctct gtagagatga tggggcggaa cgaaaagtgt
     8641 tatggattaa gagaggaacg gtgttccaag gaaacggtga atctgggtat gccccaggaa
     8701 atatcagcgg gcagagcctg tggtatcaag aagcattacc tgacatccaa tgtctcttgc
     8761 tggctcacat tcagactgcc aaaggaagca gtgggtctag cgaaaaaggt tgcgatcgta
     8821 ggagatttca ataaatggga taaagaggcc tcaccgatga agaagcttag aagcggcgac
     8881 tttgaaatca cgttagagtt gtcgacgggg agggaataca ggttccgtta tctaatggac
     8941 ggacatcgat gggaaaatga ttggtgtgcc gacaggtatg aacggaatcc ttatggcggt
     9001 gacgattcag tcgtgatagt ctgaaacact tctgccggag acagccggaa ttagtactac
     9061 gcaacgcttc ccatcgattg caacagtgaa catttccgag caaaccactc attgtattca
     9121 cgacaaccag ctacaatgaa acacgaacgc aatgtgacct gccgaagcgc gtgtctccgc
     9181 attattcagg aactatactc tcatcaataa acagtcgtat tcatttattc tccctgaatc
     9241 acgattcgat ggatttatgc caagcactcc aaagccgttc ctttcataag cagagatgct
     9301 ctctgtccag tggggcattc tttcatatgt ggggacaaga gacaacttcg tctgaaccgc
     9361 catgacgcat tcaagtttgt ttcccaagcc cttgaggact tcggtgtcgt tggcttgtat
     9421 ctccgtttta agaaaaatgt gtttgctctc aatgccaggg acaatctcgc ctagcacttc
     9481 cccaagactt tggacggaca ccttttcttc tgtagctatt gttccctcac cagaacactt
     9541 cgtacaatat tcacccgctt ttcgtaaggg actatagact gtttcgtcgt aaacattaat
     9601 tgtttggtta ggcccctgac gtcctgatga gcatttgtga atatgccaat tgcgaccaga
     9661 aaaggccgtt accgctaatc gttcaaaaac agatgagact ggtctgaatg aaagtatctc
     9721 tccggtgtag aaggcccgca gctttggagc taactggcca tcgtttaagc caaggtaaat
     9781 tacgagatca atggccattt ccttaaaaat cgacctgatc tcagcgtcat atgcaaacca
     9841 atcatcttta gttcgcttcc ggtcaattag cgtgaacaat ttgcctccac tccattgcat
     9901 gtcgtaatcg gtaaggtttt caatgaggct tcaaatccgt ttcccgaacg tgcccatggt
     9961 gttttcctct ttaaaggtcg cagtgggaca tccggcaggg ccggagatcc tcatagagtt
    10021 tccccgacag gacatttctt gtgatcttca ctgctcatat caaactccgt ctttgcagcg
    10081 aagttccatt gcgaagcagt gcacagcctt agagtagtgt attcccgtta caccatgatg
    10141 aaaatcccgt gaagattttg ttacgaggag attgatcaaa aaatatattg agctggcctt
    10201 ctttttgcag gcaataccac gaatgcgcct tagcctttgg agtgttcgtt taaccgactg
    10261 atccgcaaaa accaccttgc tgaagggatc acccttttca agagaaccat gcttatctgc
    10321 gacaaaggaa taacggtgat gtacatccag gatatcacac agacagtttt ctgctcaaat
    10381 gaggggtttc tggcggaact gaacggcgtg tgcaatttga tccgtgtcag gcacgccttc
    10441 tcacgaacac tttttcacgg agcatgtctt ttcatatctt caccctaaga cggcctcagc
    10501 atcttgctat taaggaacat gaggaataag gcatttgaga gccgttgatc tatgctgctt
    10561 ttctcgatgg tctgttctgc actgtaacga aaatttaacg cgtccttcat catcttgcaa
    10621 caaagatctg gttgaataga tactgttagc ttaaaggtat gggcattttg ctgacctttt
    10681 gaaggaacca gaaaacccgg aagacgaacc tagcaggttc ttttccgggt tttttatttg
    10741 aaaaattcaa gaggagcacc cccctcgggg aatagttacg gggatgttgt tgacctcatg
    10801 caagacacta aaagtcctga tcggtccaat gatatgccgt aatgagcatg gataaacagt
    10861 aaactgggta cgttgccgac ctgcatgttc aatgtgcagg gaccggatag cccggatgac
    10921 gagcatcgcg tgcggtgtcg aaatccgggt tttttgtctg tacggcgcaa tcaggagaaa
    10981 gtaaagccaa acccatcgta aggtggggac ggaaagccac gggtcccaca cggacagccg
    11041 ggttgcctct ctcaggagag actctccggc ttttttattg tgagctaact aaacgaggtg
    11101 aaaaaaggat aacactctcg gtaagtgaaa tcgcaggctt tgttgtcacg cagtagatgc
    11161 tttcaggaac gcacccctta ccataaaggc aaaatgccgt tcgttgcacg gtcaactggc
    11221 tgctcgcacg ggcaggcgga gcagagttgg aagcagaggt tgttacagca agaacaatgc
    11281 taggtaaaaa gaaaggagag gagaaaatga gaaagttgat tgcagcagca tgcatggctt
    11341 tgttgtttac ggcaggaacg gcaaaagcag acatagcata tccgggagct attgcttatt
    11401 ggaaccagta tggccaaaca ggtcagcagg tgtcaacacc tgggactggg tcatctaaca
    11461 ttacggcaat agatatgacc agaggggttg ggttactcac cgacagcgct ggcgctaact
    11521 cattgaattc caacggatgg accggtgagg caacggatta tattcagttt ggtttaacga
    11581 ttgcttctgg ctatgaggca aacctgaacc aactcttga
//
LOCUS       DATKOU010000058         6407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_71991, whole genome shotgun sequence.
ACCESSION   DATKOU010000058 DATKOU010000000
VERSION     DATKOU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71991"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VMU21_02690"
     CDS             complement(<1..1271)
                     /locus_tag="VMU21_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76460.1"
                     /translation="MGPLETAKKFFRLSTTLEKMVLGFYDRLPLALRYRISYGPTFFY
                     WLAFLKEAENWDRDRTIAYQFEQLKNLLLHAMRHVPYYRKLFSDYGFNPGNLQCIEDI
                     RILPYLDKETVRDRTDEFVDERIPKNFLLRDRTSGSTGIPLTIFRTKESQEIFEAFLI
                     YSFNRIGYTTRSREVLFRGMIESGKSTNLPYLRYGNKLFLSARYVMVDEWLKRYVQMM
                     TEFKPEFITGYPSVLTVISLFIKNKKLSFDKVKAIKYFAETLYDWQKDLIEGVFHRRI
                     FSVYAMTEHLLFGSECEYSMSQHLYPYYGITEFDASGGGREEIVGSGFLNYAMPLIRY
                     KTSDIALRGEDFCPLCGRHHQLIDRIEGRINDYLISREGKIIPRLMPWVKIFPNTRQY
                     QFFQDEPGKAYLKIVRAETYSEADTLYIKSK"
     gene            complement(1302..2330)
                     /locus_tag="VMU21_02695"
     CDS             complement(1302..2330)
                     /locus_tag="VMU21_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76461.1"
                     /translation="MTGPTQKKLPLVSVIIPTINNGRFIGSALESLFRQTYPGERREI
                     IVVDDGSTDNTPEALKKYGQNIFQIHQQHRGIASARNTGISHARGEIITFLDSDDLWY
                     EERLQKVVERFVENPRAGMVYHPVELIDSEGNTIVHNFYYAFGYKEEISGWVANEIFS
                     GRIYCGGSSFAFRKAIIDRIYPIPEDIKRGVDYYMIAFSSCFTEAEYILDILGKYRFH
                     GNNISMWAGRNNRKDLAAFNKDFAYMRGKLLEQIIGFQDVDAKKVDLNIIRRRQEQER
                     IFYHTLDGERVKGIKRIPSLFKGRPDHRDLLRGMAICFMALFIPAFGFSKLLKLYENL
                     KRLQIIKF"
     gene            complement(2505..3863)
                     /locus_tag="VMU21_02700"
     CDS             complement(2505..3863)
                     /locus_tag="VMU21_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HUO76462.1"
                     /translation="MMELNFSHMMTELLEEKGISVRQVEALRDRIGRAHQEIIQRAWP
                     ELSFMDLTSQDTSAIKKLAEEVRNCADDFLVLGIGGSAIGPRSILEALSPFHNLTKKP
                     RVFICDNPDPRTLNGVFSLIDMKKTVVNVITKSGSTAETMASFMILWDELSRTLGDKA
                     RERIIVTTDPVKGNLRRIATDLGLKSLSIPPGVGGRFSVLSPVGLLLSEVIGVGGAEM
                     LKGAADIHRKCSDPEIWKNPAYLFGTLLYLMEKEEKRNINVIIPYADGLKFLSEWFCQ
                     LWAESLGKLGTGITPYPSLGCVDQHSQLQLWMEGPEDKVCIFLKIMDYGTDIRIPNVF
                     EKMEGTHYLGDHLLSELMNAEEESTELALAKNKRPHMTLSIPSIDPYHMGQLFYFFEI
                     ATVFTGFLYSVNPFNQPGVEEGKNYTYGMMGKEGFEERRKEAEMAREKMLCYRLSAGY
                     DE"
     gene            4043..5662
                     /locus_tag="VMU21_02705"
     CDS             4043..5662
                     /locus_tag="VMU21_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76463.1"
                     /translation="MDKIGSLLFKEKGTIYKEPGGKITTCLVYPNTYHIGMSNLGFQG
                     IYTLLNRSPDIVCERAFLPDQEDMNEHIRTGTEILSLESKRPLNRFDIVAFSVSFEND
                     YPHILTILRMAKIPVRAKERGEGHPLLIMGGACSFFNPEPVAEFFDLCVIGEAEEVLP
                     ELMEAHRASRNREELYERAKGIEGIYLPKFYRLHYFPDGRISEREAWGTAPMMIKRRF
                     VKDISRYRTGPSIITPETEFSRMYLMEAMRGCPWNCRFCVAGSVYKPVRKKEVSTLKE
                     EIREASSATGRIGLIAPSLTDYPHTKDVLSIEGVDFSITSLRASQKSAELIPFLKGNR
                     SVSIAPEAGTERLRTVIDKKITEENILETSRLILAEGIETLRLYFMVGLPTEDQRDIE
                     GIVSLVKKLRKISHRGNIVLTLSTFVPKPFTPFQWHPMERMETVKDRLKSVKKGLIAL
                     KGVKVFHDVPKYAYMQGLFSRGDRRVSHVLEGMEGIEDWRKACANKGTNAEFYLFRQR
                     YLQETLPWDFIDNYISKEKLWTEYEKAIGNV"
     gene            5726..>6407
                     /locus_tag="VMU21_02710"
     CDS             5726..>6407
                     /locus_tag="VMU21_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HUO76464.1"
                     /translation="MSDEMKATRLIDTVSTIYKRITNAAMRAGRNPEEVKLIAVTKTV
                     TVLQIKEAIDAGLRTFGENRVQEAKAKIEELSAGWTMPSVSWHLIGHLQKNKAKYSVR
                     LFDLIHSLDSAELAEELNSQAEKIGKVQEVLLEVKLSPEETKHGVARTELPALLKMVQ
                     TMRHLRLRGLMTIPPFFEHPEEARPYFRKLRELRDEADSAGYPLPELSMGMSNDFEVA
                     IEEGATMVR"
BASE COUNT         1686 a         1576 c         1452 g         1693 t
ORIGIN      
        1 atttttgact ttatgtaaag cgtatccgct tcagaatacg tttcggctct tactatcttt
       61 aaatatgcct tccccggctc gtcctggaaa aactgatatt gcctggtgtt aggaaaaatc
      121 tttacccagg gcataagtct cgggatgatt tttccttccc gactaatgag gtagtcgttt
      181 atccttccct ctatcctgtc gatgagctga tgatgtcgtc cgcaaagagg gcaaaaatct
      241 tcgccccgga gtgcgatgtc ggatgttttg tatcttatca gcggcattgc atagttcaaa
      301 aaaccgcttc ccactatctc ttcccggccg cctcctgatg catcaaactc cgttatcccg
      361 taataagggt aaagatgctg gctcatcgaa tattcacatt cactgccgaa taataaatgt
      421 tcagtcattg catatactga aaatatcctc ctatgaaaga ccccctctat taaatccttc
      481 tgccagtcgt acaatgtctc tgcaaagtat tttatcgcct ttaccttatc gaaggagagc
      541 ttcttgtttt taataaatag tgaaatgacc gtcagcactg aaggataccc tgtgataaac
      601 tccggcttaa attctgtcat catctgaacg tatctcttaa gccattcatc caccatgacg
      661 tatctggctg aaaggaacag cttattgcca tagcgcaggt aagggagatt agtgctcttc
      721 ccggattcta tcatacccct gaatagcacc tctctgctcc gggtcgtata ccctatcctg
      781 ttgaacgaat atattaagaa cgcctcgaat atctcctgcg attcttttgt tctgaatatg
      841 gttaagggta tcccggttga tccgctcgta cggtccctga ggagaaagtt tttcggtatt
      901 ctttcatcca cgaattcatc agtcctgtcc ctcaccgtct ccttatcgag atagggaaga
      961 attcttatat cctcgatgca ctgaagattt cccgggttga atccgtaatc ggaaaaaagt
     1021 ttcctgtaat aagggacgtg tctcattgca tgcagaagga ggttcttgag ctgttcgaat
     1081 tgataagcaa tagtcctgtc tctgtcccag ttctccgctt ccttcaaaaa agccagccag
     1141 taaaagaagg ttggaccata agatattcga tatctcaggg ccagcggcag tctgtcataa
     1201 aaccccagga ccatcttctc taaagtggtc gatagcctga agaacttctt tgctgtttca
     1261 agtggaccca tttcttattt tctctttatc tattaatgcc ttcagaattt gataatttgc
     1321 aaccgcttca ggttttcata taacttcagc agtttagaga aaccaaatgc agggatgaac
     1381 agcgccataa agcatatagc catgcccctt aacagatctc tatgatccgg cctgccctta
     1441 aaaagtgagg gtatgcgttt tattcctttt accctttcac catcaagtgt atgatagaaa
     1501 attctctcct gttcctgtcg ccttcttata atatttaaat ctactttttt cgcatcaaca
     1561 tcctgaaacc ctataatttg ttctagcagc ttcccccgca tgtaagcaaa atccttattg
     1621 aatgccgcga gatctttacg attatttcgt ccggcccaca tactgatatt gttcccgtga
     1681 aatctgtatt tgccgaggat gtcgagaata tattcggctt ccgtaaaaca tgaagagaat
     1741 gcaatcatat aataatcgac acccctttta atatcctcag gaatcggata tatcctatcg
     1801 atgatagcct ttctaaaggc aaatgagcta cctccgcagt atatacggcc cgaaaatatt
     1861 tcattagcaa cccagccgct gatttcctct ttatacccaa aggcataata aaaattatgc
     1921 acgatagtat ttccctcgct gtcaatcagc tcaacagggt gatacaccat ccctgcacgg
     1981 ggattctcca caaatctctc aacaactttc tggagtctct cctcatacca tagatcgtca
     2041 gagtcgagaa acgtaattat ttctcccctg gcatgggata tcccggtatt ccttgcactt
     2101 gcaatccctc ggtgctgctg gtgaatttga aaaatgtttt gcccgtactt tttgagggct
     2161 tccggggtgt tatcagtgga gccatcgtca acgacaatga tttccctcct ttccccaggg
     2221 taggtctgtc gaaagaggct ctccagggca gacccaataa aacgcccatt gttaatggta
     2281 ggaatgatta cagaaaccag tggcagcttt ttttgggtcg gtccggtcat ctcaaaaatg
     2341 ctcttctctc ctcatatgca attctcgggt gaccgttctg tcctttttcc ctcacgattc
     2401 gggctccact ggtttaatgg gagcttttat gtcgggatac gccctgaagg tgaggattcc
     2461 atgaagggga tcggtattgt ccaatcttct caaatatcac gagttcattc atcgtaccct
     2521 gctgacagtc ggtaacagag cattttctcc ctcgccattt ccgcctcttt tcgcctctct
     2581 tcaaacccct ccttgcccat cattccgtag gtgtagttct tgccttcttc cacccctggc
     2641 tgattaaagg gatttacgct gtagaggaat cctgtaaaca cggttgcaat ctcgaagaaa
     2701 taaaacagct ggcccatatg gtaggggtct atggaaggaa tgctgagagt catgtgaggt
     2761 ctcttgtttt tcgcaagggc aagttctgtg gactcttcct cagcattcat gagctccgag
     2821 aggaggtgat ctcccagata atgggtcccc tccatcttct caaaaacatt ggggatccta
     2881 atatccgttc catagtccat gatcttcaga aatatacaga ccttatcctc gggcccttcc
     2941 atccagagct gaagctggga atgctgatca acgcatccga gggacggata cggcgttatg
     3001 ccggtaccga gtttcccaag gctctcggcc cataactggc aaaaccactc agacaggaat
     3061 ttcagaccgt ccgcataagg aatgatcaca ttgatatttc ttttctcttc tttctccatc
     3121 agatacagga gcgtcccgaa cagatacgcc gggtttttcc atatttcagg gtccgagcat
     3181 tttctatgga tgtccgcagc acccttcagc atttccgcgc cccctactcc tattacctct
     3241 gagagcaaaa gaccgacagg gctgaggacc gaaaacctcc cccccactcc gggagggata
     3301 gagagagatt tcaaccccaa atcagtcgca atccttctga ggtttccttt gacagggtct
     3361 gtggtcacaa taatcctctc ccttgcctta tcccccaaag tccttgaaag ttcatcccag
     3421 agtatcatga atgatgccat ggtctctgcg gtacttcctg atttagtgat gacattgaca
     3481 acggtcttct tcatgtcgat tagagagaac actccattga gcgttcgcgg atccggatta
     3541 tcgcaaataa agacccttgg tttttttgtc aggttatgga acgggctcag ggcctccaga
     3601 atcgaccgag ggccaatggc agatcccccg attccgagga ctaagaagtc atcagcgcag
     3661 ttccttacct cttccgcgag cttttttatc gcagaggtat cctgggaagt aaggtccata
     3721 aaagaaagct caggccatgc cctttgtata atctcctggt gggccctccc gattctgtcc
     3781 ctcagggcct caacctgcct tacagagatt cctttctctt cgagtagttc ggtcatcatg
     3841 tgagagaaat tcaattccat catcctgttt catcccctct caagggtgcc ttcgcatccc
     3901 cgtctttcca cactttatca tgtatcttta ccaatgctcg aagaaatgtc aattctttaa
     3961 aaacacttcc gatcatctga ggtcatcagg ctatgggagt tgcggttcat gtatactatc
     4021 ttcaatgact agcaaaagga taatggacaa gattggatcc cttcttttca aggaaaaagg
     4081 aaccatttac aaggagccgg gcggtaagat aaccacctgc cttgtgtatc cgaacaccta
     4141 ccatatcggg atgtcgaacc tgggttttca gggcatctat acccttctga acaggagtcc
     4201 cgacatcgtc tgtgaaaggg cctttctccc tgaccaggag gatatgaacg aacatataag
     4261 gacagggacg gaaattcttt ccttagagtc aaaaagaccc ctgaaccgct ttgatattgt
     4321 tgcattctcg gtctcttttg agaacgatta cccccacatc cttaccattc tccgaatggc
     4381 gaagattccg gtgagggcaa aggagagagg agaaggccat cccctcctga tcatgggcgg
     4441 ggcctgcagc ttcttcaatc cagaaccagt ggcagaattc tttgacctgt gcgtaatagg
     4501 agaggcggag gaggtactcc ctgaactcat ggaggcacac agggcctcaa gaaacaggga
     4561 agaactctat gaaagagcga agggtataga agggatctac ctgccgaagt tttaccgcct
     4621 ccactatttc cccgatggaa ggatctcaga gagggaggca tggggaaccg cacccatgat
     4681 gataaaaaga cgttttgtga aggatatatc gagatacaga accgggccgt ccatcatcac
     4741 gccggaaaca gagttctcac ggatgtatct catggaggcg atgaggggtt gtccatggaa
     4801 ctgcaggttc tgtgtggcag gctcagtcta caaaccggtg aggaagaaag aggtgagtac
     4861 attaaaagaa gaaataagag aagcctcatc cgcgaccggc agaataggcc ttattgcgcc
     4921 atcacttacg gattatcccc acacaaagga tgtcctcagc atcgaagggg ttgacttctc
     4981 catcacctca ttaagggcaa gtcagaagag cgcagaactt atcccgtttc tcaaagggaa
     5041 caggagcgtc tcgatcgccc cagaggcagg caccgagagg ctgaggacag tgattgacaa
     5101 aaagatcaca gaggagaaca tcctggaaac ctccaggctt atccttgcag aagggattga
     5161 aaccctcagg ctttatttta tggtgggcct tcccaccgag gaccaaaggg acattgaagg
     5221 gattgtctct cttgtgaaga agctaaggaa gatttcgcac agggggaata tcgtccttac
     5281 tctcagcacc tttgtcccca agccgttcac cccctttcag tggcatccca tggagaggat
     5341 ggagaccgtg aaagaccgcc tcaaatcagt gaagaaaggg cttattgcct taaagggggt
     5401 gaaggtgttc cacgatgtcc ctaaatatgc atacatgcag ggactcttct cacggggtga
     5461 caggagggtt tcccatgttc tggaaggtat ggaaggcatc gaagactgga gaaaggcgtg
     5521 cgccaataaa gggacaaacg ctgaatttta tcttttcagg cagagatatc tccaggaaac
     5581 acttccgtgg gatttcatcg ataattacat ttcgaaagaa aaactctgga cagaatatga
     5641 aaaggcgatt ggcaatgtgt gaggtgagaa gcgaattcat tctccgtgct gcattcccct
     5701 tctccggaaa ccggataaga gcatcatgag cgatgagatg aaggcaaccc ggctcatcga
     5761 caccgtaagc actatttaca aaaggattac caatgcggcc atgagggcag ggaggaatcc
     5821 tgaggaggtc aagctcattg cagttacgaa gacggtcacg gttctccaga tcaaagaggc
     5881 catagatgcg ggattaagga cattcgggga aaaccgggtc caggaagcaa aggccaagat
     5941 tgaagaactt tcggcaggat ggacaatgcc ttcggtctca tggcatctca taggccattt
     6001 acagaaaaac aaggcaaaat attcggttcg gttattcgac ctgatccact cccttgactc
     6061 tgcggagctt gcagaagagc tgaactcaca ggcagaaaag atcggtaagg tccaggaagt
     6121 gctgctggag gtgaagctct cacctgagga gactaagcat ggagtagcca ggaccgaact
     6181 cccagcgctc ttgaagatgg tccagaccat gagacatctg agactgaggg gcctcatgac
     6241 aatccctcct ttttttgagc accctgaaga ggcgagacca tacttccgga agttgaggga
     6301 attacgggat gaggctgatt ctgcagggta ccctctgcca gaattatcca tgggtatgtc
     6361 caatgatttc gaggtagcca ttgaagaggg agcaaccatg gtcagga
//
LOCUS       DATKOU010000059         5878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3920026, whole genome shotgun sequence.
ACCESSION   DATKOU010000059 DATKOU010000000
VERSION     DATKOU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920026"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..889)
                     /gene="pstA"
                     /locus_tag="VMU21_02715"
     CDS             complement(50..889)
                     /gene="pstA"
                     /locus_tag="VMU21_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HUO76465.1"
                     /translation="MNLERKRKLESLTALVLSTLAAGFGLFWLIFILGDVLIHGIGAL
                     NISLFLHDPTPAGVTGGGLRNAFVGQLLITSCATLIGVPIGVLGGTFLAEYARGTAIS
                     RVVSILADIMVSVPSIVIGTFAYAILVRPVGHFNGWAGSVALAIIMIPVVLRTTEDML
                     SLVPWTLREAAFALGAPYYKVIIQVVYRGAATGILTGILLSIARVAGETAPLLFTSFN
                     NAYFSLDMNRPIASLTASIFQYAMGPYDDWHAQAWAASFIITLFVLAITILGRLIIKW
                     RYK"
     gene            complement(886..1827)
                     /gene="pstC"
                     /locus_tag="VMU21_02720"
     CDS             complement(886..1827)
                     /gene="pstC"
                     /locus_tag="VMU21_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HUO76466.1"
                     /translation="MPIIYKKNTVDFSFSLVTALASLTVILLVMGIFAVLLRESFLSI
                     SKFGVVHFLTSTDWNPVKESFGAATTIYGTVVTTFLALLIAVPTAIGIAIFVTEISPN
                     FLKGPIGIAIELLAAIPSIIYGMWGLFALAPIMSRYIEPALQKSVGKLPFLGVLFQGT
                     PLGIDIFTASVILSIMIIPFTASISRDAFNLTPAVVKESAYAIGTTQWEVVKDVILPY
                     SKLGVFGGIALSLGRALGETMAVAFVLGNNHQITSSLFQAAATITVSLANEFTEADSD
                     MYLSSLFYLALVLFVLSFVVLAIAKFFLLKAERRYSR"
     gene            complement(1972..2982)
                     /gene="pstS"
                     /locus_tag="VMU21_02725"
     CDS             complement(1972..2982)
                     /gene="pstS"
                     /locus_tag="VMU21_02725"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HUO76467.1"
                     /translation="MKKILGVLAVLTLVLSFSWANAEETLNGAGASFPYPVYSAWAYD
                     YNKVTGVKLNYQSIGSGGGIKQITARTVDFGASDAPLNPEQLTKDNLLQFPAVIGGVV
                     PVVNIPGIKAGELKLDSGAVCKIYLGEIKFWDDGMLKKMNQKVSLPHKEITVVHRSDG
                     SGTTAIFTEYLSGVCPDWKSKVGAGTSVQWPVGIGGKGNEGVANYVKQTENSIGYVEF
                     AYAEQNKLAYALLKNPAGNFVKPSFEAFEDAAATGDFDPQKDFHLWLVNAPGKHSWPI
                     AGATFILLAKEKADSNKNVIKFYDWAFKNGDNKAKELVYVPLPKSLKDKITAYWKAHG
                     LY"
     gene            complement(3520..3711)
                     /locus_tag="VMU21_02730"
     CDS             complement(3520..3711)
                     /locus_tag="VMU21_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76468.1"
                     /translation="MKCPHLNRWIVATCKIDDRTYVPSVFQLDEYCKTGGHKRCPFFV
                     KNVSEKKETSGQVPVGEYA"
     gene            complement(4102..4365)
                     /locus_tag="VMU21_02735"
     CDS             complement(4102..4365)
                     /locus_tag="VMU21_02735"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76469.1"
                     /translation="MIQYRIVHHVPGRIRIEVPSIKGLHLKNLARLSAIPFPSGIEGV
                     RPNPLTGSLLIKYDPEQINIVTYLQEMAVSSVLEDKLTGGDTP"
     gene            4605..4895
                     /locus_tag="VMU21_02740"
     CDS             4605..4895
                     /locus_tag="VMU21_02740"
                     /inference="COORDINATES: protein motif:HMM:NF028504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5132 domain-containing protein"
                     /protein_id="HUO76470.1"
                     /translation="MGLFDDVFKGNVVTGLAIGIGAAVLGPAVLPVIASVAKPVAKAA
                     IKGGIILYEKGKEAFAEMGEVVEDLVAEAKSEVEEAHKAAAAAAAPQGETEG"
     gene            4895..5374
                     /locus_tag="VMU21_02745"
     CDS             4895..5374
                     /locus_tag="VMU21_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76471.1"
                     /translation="MLPIAHISHQASGRLRIRIPSKKGSNSYFNALKEHFSGLEGINA
                     LEVNPTTGSVLIVHNLDRQTIEQYATAKNLFSLTVAHSSPAGLHPKVSETFQGLNNQM
                     KAFTSGEINIGGMAFLALMGAGIYQIGRGNFTAIPWYTAFWYAFNIFLKSQNDSKME"
     gene            5567..>5878
                     /locus_tag="VMU21_02750"
     CDS             5567..>5878
                     /locus_tag="VMU21_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76472.1"
                     /translation="MPRENVVKGTPAEEKTSMGKAGTSVKEGVVSSLKGINEIEAEIV
                     SLVRNTVSNTLRATGSVANEAVTITKDVVKGTIQATEEVGTGLILSTKSVAKGIVMGV
                     SD"
BASE COUNT         1615 a         1431 c         1370 g         1462 t
ORIGIN      
        1 gcatagtaaa aattcaaatt cctggtctct atctccctct tttcctccac tatttgtacc
       61 tccattttat gataagcctc ccgagaatgg tgatggcgag gacgaacagg gtaataataa
      121 aagatgcggc ccacgcctgc gcgtgccaat catcataagg tcccatggca tactggaata
      181 tgctcgcagt cagagaggct atgggcctgt tcatatccag agagaaatat gcgttattga
      241 atgatgtgaa gagaagcggc gcggtttccc ctgcgaccct tgcaatggaa agcaggattc
      301 ctgtgagaat gcccgtggct gccccccgat agaccacctg aatgatcacc ttgtagtagg
      361 gagcacccaa tgcaaaggcg gcctccctca aggtccaggg gaccagtgac aacatatcct
      421 ctgtcgttct taagaccaca gggatcatga taatcgcgag ggcaaccgag ccggcccacc
      481 cgttaaagtg tcctaccggc cttaccagta tcgcgtacgc aaaggtccct atcactatag
      541 agggaacgct caccataata tccgcaagta tgcttaccac ccttgatatc gctgttcccc
      601 tggcatattc ggccagaaaa gtcccgccca ggaccccgat gggaactcct atcagagttg
      661 cgcaagaagt tatcagcaac tgtcctacga aggcgttcct cagtccaccg cccgtaacgc
      721 cggcaggcgt tgggtcatgc agaaaaaggc tgatgttcaa tgcgccaatg ccgtgaataa
      781 ggacgtcgcc caggataaag atgagccaga aaagtccaaa gcctgccgca agggtgctca
      841 ggacaagggc cgtcaggctt tcgagtttcc tctttctttc aagattcatc gcgaatatct
      901 cctctcggcc ttgagaagga agaactttgc gatagcaagg acaacgaagc tcagcacaaa
      961 gagcacaagg gcgagataaa agagtgaaga gagatacatg tcgctgtccg cctcggtaaa
     1021 ctcattggca aggctcacgg tgattgtggc agcagcctga aagagcgagg aggttatttg
     1081 gtggttattc ccaagcacaa aggcaaccgc catagtctca ccgagcgctc gtccgaggga
     1141 gagggcgatc ccgccgaata ccccgagttt cgaataaggg aggatcacat cttttaccac
     1201 ttcccattgc gtagtgccga tagcatacgc tgattctttt accacagccg gagtgagatt
     1261 aaaggcatcc ctcgatatgc tcgcggtgaa ggggataatc atgatgctca aaataacgct
     1321 cgcagtaaag atatctattc ccaagggtgt gccctgaaac aaaacaccga gaaaggggag
     1381 ttttcccaca ctcttctgga gcgcaggctc aatatagcgg gacattatcg gcgcaagggc
     1441 gaagagcccc cacatgccgt aaataatgct gggtattgct gcaaggagtt ctatcgcaat
     1501 gccgatggga cccttcagga agtttgggga tatctccgtg acgaatattg cgatcccgat
     1561 ggcggtcggc accgcaatga ggagggcaag aaaggtcgtt actactgttc cgtaaattgt
     1621 ggtggccgcg ccaaaggatt ctttcacagg gttccagtcg gtagaggtga gaaaatgaac
     1681 gactccaaat ttgcttatgg aaaggaaaga ctcacgcagc aagaccgcaa aaatccccat
     1741 aacaagaaga atgacagtca aagacgcgag tgcggtaacc agcgaaaagg agaaatcaac
     1801 cgtattcttt ttgtagatta tgggcacacc ttactccttc cgccgtcttg acagccagcc
     1861 ggccatcacc atcaaactct cttcatcacg tagtttctaa agattattaa agggccaggt
     1921 atatgataca gacctggccc cttaaatttc aaattacctt aaactttact tttagtacag
     1981 cccgtgcgcc ttccagtaag ccgtgatctt atccttgaga gatttcggga gagggacata
     2041 gacaagctct tttgccttgt tatccccatt cttgaacgcc cagtcataaa atttgatgac
     2101 attcttattt gaatcggcct tttcctttgc caggagtatg aatgtcgcac ccgctatggg
     2161 ccatgaatgc ttgccgggag cattcacaag ccagagatga aaatcttttt ggggatcaaa
     2221 atctccggtg gctgccgcat cctcaaaggc ttcgaagctc ggttttacaa aattacctgc
     2281 cgggtttttg agaagggcat atgcaagctt gttctgctct gcatacgcaa attccacata
     2341 tccaatggag ttctcggtct gcttcacata attggcaacc ccttcgttcc ccttgccgcc
     2401 aatccccaca ggccattgaa ccgaggttcc ggctcccacc ttgctcttcc agtcagggca
     2461 gacaccgctg agatattcag taaatatcgc agtcgtcccc gaaccgtccg acctgtggac
     2521 cacggtgatt tccttgtgcg gcaggcttac tttttggttc atcttcttga gcatcccatc
     2581 gtcccagaac ttgatctccc ctaggtatat cttgcagaca gcgccagaat caagcttaag
     2641 ctcacctgcc ttgatgccag gaatattgac cacaggaacc actccaccga taaccgccgg
     2701 gaattgtagc aaattatctt tggtaagctg ttccgggtta agcggtgcat ctgatgctcc
     2761 gaagtccaca gtccttgctg tgatctgttt gatcccgcca cctgaaccga ttgactgata
     2821 attgagcttt acacctgtca ccttgttgta atcatacgcc catgcggaat ataccggata
     2881 agggaatgat gctcccgcac cgtttaatgt ttcttccgcg tttgcccatg aaaacgacaa
     2941 cacaagagtc aataccgcca atacacctaa aatcttcttc atccattttc tccttttttt
     3001 ttgtattcta tttctattaa tagaataaac ttccgtctgt tacaattgtg ttacaattcg
     3061 gttaaatttc agttacatca tgcaaacatt cctttttatt cacctccttt tgctcttcac
     3121 caaagacttt ttggatgagc tgaaagacaa aaagatttgg tcttcccctg aacagaagga
     3181 ccaaaacagc tgaggaaatg atcaggctga atagcagtat ggaaatgttc acggcatgta
     3241 tgttaccaaa aggattgtta cagcaatatt aagggggtgt tacatttaag aaattttctt
     3301 tgaactatag agataagttg ctgatgtccg gggaagcctg ccgatatgca ctgatcaaga
     3361 gttccatatc ttgtatcctc gtgagatctt cccgtcccac caccgagcaa tccttaacaa
     3421 tgctggagcg gtcttccctt aggccttcaa accatgaaat acataaccca gactgacccc
     3481 tcacaagcat gaccgcattg agaaaaaatg aggggtattt catgcatatt cgcccacagg
     3541 gacttggccg ctcgtctctt ttttctcgct cacgttcttc acaaagaaag ggcacctttt
     3601 atgtcccccg gtcttacaat attcatcgag ctggaacaca ctcggtacat aggtcctgtc
     3661 atcgatttta caggtggcca cgatccaacg atttaagtgc gggcatttca tgatctcctc
     3721 caaatatgca aaagtgattt aggtcgtaaa acatcccttc cctttgattg gtatttctcc
     3781 aatatgataa aaggacacat ctcatttttc ccctggcaaa aaaaactgcc gaattcagcg
     3841 ctcacaggga aacccctcag gcatctgcac tcttcccgga tgtaatcaat gcacgccatt
     3901 cctacctccc cctcaggact tttgccagga atatgggaca gttgtcatag tcctcgttgg
     3961 cacaatgttt tttcctcatc ctgtgggatg gcaccattcc agaaagagca gcggtacacc
     4021 catcagacat gtctaaatat ggacaccgca actcatcatc tttgctccat ccatcttgca
     4081 gtgacaggac atgatgaaca ctcatggagt gtcccctccc gtgagtttat cttccaaaac
     4141 actgcttacc gccatctcct ggaggtatgt cacaatattt atctgttccg gatcatactt
     4201 gatcaagaga cttccagtga gtggattcgg acgcacgccc tctatgccgg acggaaaggg
     4261 aatggctgac agccgtgcga ggttcttcag gtgaagtccc tttattgagg gaacctcaat
     4321 cctgatgcgg cccgggacat ggtgtactat cctgtactga atcatctctc attctcctcc
     4381 ttcttgttat cattacggac attaaaccat ccctttgtta aagggatatg aatacaaagt
     4441 tacattttgg tgaaaaatcc ttgcgcaagt aggcaagtaa tgaaaaaata catggcgacg
     4501 agaataggag catgataggc taattaatac agagaaaagt ggttatctat tttttttgag
     4561 actatctttt cttaaattac gcctattaaa taaggaggaa caggatggga cttttcgatg
     4621 atgtgttcaa aggaaatgtc gtaacgggtc tcgcgatcgg cataggtgct gcggtccttg
     4681 gtcctgcggt gcttcccgtt attgcaagtg tggccaaacc agtggcaaag gctgcaataa
     4741 aagggggtat catcctttac gaaaaaggca aagaggcctt tgcagaaatg ggtgaagtgg
     4801 ttgaggacct cgtagcagag gcaaaatccg aagtggaaga ggcacacaag gcagcggctg
     4861 cggcagctgc gccgcagggc gaaacggaag gttgatgctt ccgattgctc atataagcca
     4921 tcaggcctca ggacgactga ggatcagaat accctcaaaa aaagggagca atagttactt
     4981 taatgcattg aaggagcatt tttcagggtt ggaaggcatc aatgccctgg aggtcaatcc
     5041 aacgacggga agtgtgctca ttgtgcacaa cctggacagg caaaccattg aacaatatgc
     5101 cacagcgaag aatcttttca gcctgacagt agcgcattcc tctcctgccg gtttacaccc
     5161 gaaggtgtcg gagacttttc agggcctgaa caatcaaatg aaggcattca cgtccgggga
     5221 gatcaatatt ggaggaatgg catttcttgc ccttatgggt gcggggatat atcagatagg
     5281 cagagggaat ttcactgcca ttccctggta taccgcattc tggtacgcct tcaatatctt
     5341 tctaaaatcc cagaacgatt caaaaatgga ataagccctt tgacagattt aacataattg
     5401 taaaactaac ttcactaacc tgtaacagag aggctgtaac attgtggttg atttaagcag
     5461 catacctcag cattccctta tgattattct ttaatggaac agatagctta tggcatatga
     5521 gaattttata aacaaacaat gcatttaagc ggaaaggagg cataatatgc caagggaaaa
     5581 cgttgtaaaa ggcacaccag cagaagagaa aaccagcatg ggtaaagcgg gtacatcagt
     5641 gaaagagggt gtagtgagca gcctaaaggg tatcaatgag atcgaggccg agattgtgag
     5701 cctggtgagg aacaccgttt ccaataccct gagggcgacc ggctcggtgg caaatgaggc
     5761 ggtcaccatc acaaaagatg tggtaaaagg gacgatccag gccacagaag aggtcggcac
     5821 aggccttatc ctcagcacaa agagtgtcgc taaaggaatc gtcatgggtg tgagtgat
//
LOCUS       DATKOU010000060        22794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1999662, whole genome shotgun sequence.
ACCESSION   DATKOU010000060 DATKOU010000000
VERSION     DATKOU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 22794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22794
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999662"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VMU21_02755"
     CDS             <1..218
                     /locus_tag="VMU21_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76473.1"
                     /translation="QKILREALRLLRRGGKLLIIDWKKEQTPEGPPLEIRVTEEAIES
                     QMLKAGFRDIVKYAVLPYHHFLVGEKV"
     gene            complement(478..2259)
                     /locus_tag="VMU21_02760"
     CDS             complement(478..2259)
                     /locus_tag="VMU21_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HUO76474.1"
                     /translation="MKNNEEVASGQEKESLKKFFPPLQWWPAVSRRSTRADIIAGITG
                     AIIVLPQGVAFAIIAGLPPEYGLYSAIVPAIIAALFGSSHHLISGPTTAISIVIFTAL
                     SPLAPPSSAEYIQMALTLTFLAGFFQLALGVARLGALVNFVSHSVVVGFTAGASILIA
                     TSQLSNILGITAPIKHSFIHIWADMLSRLSETNLYVLSIGLSTLIIAVVLKTLKPKWP
                     GMLIAMIAGSVMALLMKGNEHGIRLVGSLPGHLPPLSHPYITTASLRQLAPPALAVAM
                     LGLAEAVSIARSIATKSEQLIDSNQEFIGQGLSNIIGSFFSSYASSGSFTRTGVNYES
                     GARTPLAAVFAAITLTGILLLIAPLTAYLPIASMAGILFLVAYNLIDTHHVASIVRTS
                     KPEAGILIATFLSTLFVELEFAIYVGVLLSLLLYLNRTSHPTFVTLAPDPESRRKTFI
                     NVQKKPLSECPQLKIIRIDGSIFFGAVNHVVEQLDQITKKYPEQAHILIVGGGINFID
                     VAGCQTLNQEAHRLRVNGRQLYLCSLKGEVIDVMKRGGCNKSIGEENVFHSKVEAIRS
                     IVPRLDPERCRRCKVRIFHECAQMPGA"
     gene            2451..3221
                     /locus_tag="VMU21_02765"
     CDS             2451..3221
                     /locus_tag="VMU21_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76475.1"
                     /translation="MIYWLKALWPALLVLTLSIVLSLLLGEFAFLRGDRAIVKGILRF
                     SRIAVLSSLTLYLVLPLNRWLLRQMKHHLLKVTDSAPPAFSPLKIWVYRPLQCIGTSL
                     LFDTKLITALQLVAGSTVSDSLLPKGRFNIESFLATSLVTAVTSLLLASIWTLDDTGI
                     RYFNRKEHELKMAGKFVGTIMPIVFGFSGVRGLLATFPTIQAMIYLFYIFIILYPPFT
                     VLSALHYHFVKRKFERFSGIGAIRKGMILETKSGDLNS"
     gene            3463..4233
                     /locus_tag="VMU21_02770"
     CDS             3463..4233
                     /locus_tag="VMU21_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUO76476.1"
                     /translation="MAGKAIWKGYINFGDVNLPVKLHTAIKEDRIQFHLLHKRDHLKL
                     RQQMICAFEKKPVPTEAQVKGFEVEEGKYIIVDPEEIEQTAPEDSRMIEVREFVKTEQ
                     IDPLFLERVYYLEPDVPLKGYDALVGALKEMEVAGICTWAMRKRYYLGALQTGGKILR
                     LTTLRYADEVISLKSLELPDIPLSEKELKIGSDLINRLTAPFQPQKFENEHQKKLQSL
                     IEKKARGEKIAVLRPRRLKPTAPDKLLQALEASLKKVA"
     gene            4234..5079
                     /locus_tag="VMU21_02775"
     CDS             4234..5079
                     /locus_tag="VMU21_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUO76477.1"
                     /translation="MKTRDPISKPSKSSSHRENEAVMQGIWSGTISFSLVAIPVDLVK
                     AVDSGRVSFRMLHSKDYSPLQRRMFCPEEETMVPPDEIIRGYEIGPDKYILISDEELE
                     SVSPERSRTIEIVEFIDMDEVDPIYYDHPYYLIPSKGGEKAYRLLVEVMRRTHKAGLA
                     KFVLGEREYLVAVYSSEGALALITLHYREEILPDEDISPKKGKIEDEEKSRIKQNIRK
                     MMADFDPGKYADKRRKEIMDLLKKKAKEKAPVEAPEMEGEEGEGPADLVAALKESMRK
                     VKKNR"
     gene            5076..5981
                     /gene="ligD"
                     /locus_tag="VMU21_02780"
     CDS             5076..5981
                     /gene="ligD"
                     /locus_tag="VMU21_02780"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUO76478.1"
                     /translation="MSRKAVEIAGRRLSLSNLEKDLYPSYGFTKAHILEYYLGIAKFI
                     LPHLKDRALTLKRYPEGVEKDFFFEKRCPSHHPEWVNTAEVSPDEGERMTVCLVNDLA
                     TLMWVENLASLELHVPLARVDSPETPDSMVFDLDPGDRANILSCARVALMLRDLLSGL
                     RLTSYVKTSGKKGLHVYVPLNRKETTFENTKTFSKAVAEIMQKNYPNLVTAKMAKEYR
                     KAKVFINWAQNDSSKTMICVYSLRAREKPVVSFPLAWKELENLSKQGDPEKLQIKHSE
                     AVGRAEKQGDLFREVIVKKQKLPYL"
     gene            5978..7636
                     /gene="ligD"
                     /locus_tag="VMU21_02785"
     CDS             5978..7636
                     /gene="ligD"
                     /locus_tag="VMU21_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUO76479.1"
                     /translation="MTKTQMGTGPFARKPDLTSRGLKEYASKRKFGKTPEPKPGLRTK
                     GGHLIFVVHKHAARALHYDLRLELEGVLKSWAVPKGPTMDPSLKRLAVMVEDHPFDYK
                     DFEGVIPEGNYGAGSVIIWDRGFYHHPSAGGRKESEKLLLDGLRKGNMKFVLAGEKLQ
                     GEFALVKTGRDGKSWLLLKKKDRYAIQGDILRENRSVVSNKTLEEVFRTKPEKSLRQK
                     KINQIRLREVLEIEDLKDAPVKPMPYSIEPMLATLGKEPFDHPDWIFEVKWDGYRAIA
                     EIRDGDVSLYSRKRTLLNQKFLPLVESLHKLGFEAVLDGEIVVVDDQGHPDFQMLQNY
                     QKSGRGHLLYYVFDLLYFQGHDLTNLPLLRRKELLKKILPSSPKIKFSDHVSKEGVSF
                     FNVVKEKGLEGIIAKHSQSAYRTGRRSRQWLKMKTRLTQEGVIAGFTEPSGGRKYLGS
                     LVLGVFERGDLIYIGHSGGGFSAKDLKEIHEKLHPLIQEKCPFKVEPETNTLVTWVKP
                     KLVCEVALSGWTEEGIMRQPVFLRLREDKAAREVVGDRPGKSNR"
     gene            7728..8717
                     /locus_tag="VMU21_02790"
     CDS             7728..8717
                     /locus_tag="VMU21_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76480.1"
                     /translation="MRHKSLIKIFILIAIPITVFAANPVSVTIKEWTLPTPDSFPHDP
                     ALGPDGSLWYTGMGSNTLGRLNPKTGEIREYSLKTANSGPHGLVADREGNVWFTANAK
                     GYIGRLNPVTGKITEYQLPKAAHDPHTPVFDRKGILWFTVQAGNMVGRLNPKTGDIKV
                     KPLPTPNARPYGIAVNSKGIPFFCELGTNKLARIDPDTMTIREYPLPRDARPRRLAIT
                     ADDIIYFTDYSIGKLGRFNPSDGKTEEWPSPGGRDSNPYAIAITPDSMVWYSESGVTP
                     NTIVRFDPKTGSFSSWPIPSGGGVVRNMAATPEGDLYLACSGKNKVAIVHVTE"
     gene            8808..9584
                     /locus_tag="VMU21_02795"
     CDS             8808..9584
                     /locus_tag="VMU21_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HUO76481.1"
                     /translation="MIGAIAGDIIGSVYEWKNIKTTEFEIFSSPCRFTDDTVLTIAVA
                     DCILNGKHYATTYREYGRRYPDAGYGGLFREWLFSDHPRPYNSFGNGAAMRVSPVGFA
                     FPSLEAVLDEARRSAEVTHNHPEGIKGAQATAAAVFLAREGESKEGIQRFIEKRFKYN
                     LHRKLEDIRPDYRFDVTCQGSVPEAITAFLESLDYEDAVRNAISLGGDSDTIACITGG
                     IAQAYYKSVPPFIVEKVRGILPDKLLKIMDDFNKVFRAAL"
     gene            9918..11432
                     /locus_tag="VMU21_02800"
     CDS             9918..11432
                     /locus_tag="VMU21_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HUO76482.1"
                     /translation="MNILLLYPEYPDTFWSFSYALKFTSKKAAYPPLGLLTVAAMLPP
                     EWDKRLVDMNVRKLKDKDLLWADYVFLSAMSVQKESVKAVLARCKELDVKVVAGGPLF
                     SNDYEEFGDVDHLVLNEAEITMQPFLNDLREGRPKPLYASAEKADMTKTPIPLWELID
                     MRHYSSMNIQYSRGCPFDCEFCNITSLYGRVPRTKSAGQLIAELEKIYSLGWREGVFF
                     VDDNFIGNKKKLKDDILPSLIQWMEERKRPFYFSTEASINLADDEELMKMMAQAEFLT
                     VFIGIETPHEESLIECDKVQNRNRDLIASVKKIQDFGLEVQAGFIVGFDQDPASIFEK
                     MITFIQESGIVTAMVGLLNAPRGTKLYERLVTENRLLKTATGDNTDLSMNFIPKMSYE
                     KLIKGYRQIIETIYSPKHYYARVKRFMKDFQPMQKKVVQLRYSYLKAGFKSVFILGML
                     RKERFYYWRLVFWSLFRKPKLLPMAVTFSIYGFHFRKIFERHFRRMERDNCAQT"
     gene            complement(11529..12404)
                     /locus_tag="VMU21_02805"
     CDS             complement(11529..12404)
                     /locus_tag="VMU21_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HUO76483.1"
                     /translation="MLLIVHGGAGERRPTKKALKTLSWSLSHGYGILENGRTALDAVV
                     GAITVLEDSGIFNAGTGGNLQLDGIRRLDASVMEGKKLKTGSVIGVEGVKNPVKLARL
                     IMDLPNTMLTNIGAKRIAKANNLDPLPGPDRRALEKLEKIKRTKKDSPRIFKNYFSTV
                     GAAALDSEGNLAAGASTGGIHAMLPGRVGDTPIIGAGVYAENSQGAVSCTGIGEYILR
                     LALAKEICMNLGSMTPRAASRRSLKRLLRTGGAAGVIVLDTQGRFSIVHSTRFMAAGY
                     AHRKGITVKEGFSLL"
     gene            complement(12552..12845)
                     /locus_tag="VMU21_02810"
     CDS             complement(12552..12845)
                     /locus_tag="VMU21_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76484.1"
                     /translation="MFEILTTDEAREQLNRLQADKGLAKRYKAVKKALLFLSKNPRHP
                     GLRTHEFTTLKGPRGEKVFEAYAEQSTPAAYRVFWYYGPEETQITIVTITPHP"
     gene            complement(12855..13103)
                     /locus_tag="VMU21_02815"
     CDS             complement(12855..13103)
                     /locus_tag="VMU21_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76485.1"
                     /translation="MPTAARRKDDHIKVKQFLTDTKGHKMAAVIDIEEFNRVKAVLNL
                     IPSSEAWLYKNKKALDSVQRGLKQAAKGKVTRLNPDEL"
     gene            13264..13623
                     /locus_tag="VMU21_02820"
     CDS             13264..13623
                     /locus_tag="VMU21_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUO76486.1"
                     /translation="MPNPFVHIELQTQDLEKSKKFYAGMFDWKLEEFPGMDYTIIEVG
                     EGTGGGMMKKPDPGILDNWLPYIMVADIAASTKKAQALGATICKEVTEVPDMGWFSVI
                     TDPTGATFGLWQPKAMK"
     gene            13687..14289
                     /locus_tag="VMU21_02825"
     CDS             13687..14289
                     /locus_tag="VMU21_02825"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HUO76487.1"
                     /translation="MNKKSRIESRRCPWVDLSKPEYVEYHDKEWGVPVFDDRTIFEFL
                     TLEAAQAGLSWYTVLRKRDHYRQAYRGFDPEKVARFNASDIERLIQNPGIIRNRLKIE
                     ASVHNARRFLEVREEHGSFSNYIWGFVNNKPRINKIKKLSDYAATSKESDALSRDLKK
                     RGFKFVGSTTCYAHMQATGMVNDHSLDCFRRKEIIRDYKS"
     gene            complement(14338..14577)
                     /locus_tag="VMU21_02830"
     CDS             complement(14338..14577)
                     /locus_tag="VMU21_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76488.1"
                     /translation="MKKKIKYSEEPMGDLRVVKDFLPPPDQLVLKEENVKVTIALRKS
                     SIDFFKEEAQKHHTSYQKMIRQVIDWYASRYKTSA"
     gene            complement(14555..14839)
                     /locus_tag="VMU21_02835"
     CDS             complement(14555..14839)
                     /locus_tag="VMU21_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HUO76489.1"
                     /translation="MKKTHFEWDKEKDMENQAKHNVSFSLAQYAFFDPKRVIVEDIHH
                     GAKEERFYCIGRVGEGIMTVRFTYRNDVIRIYGAGYWRKGRKIYEEENKI"
     gene            complement(14930..16032)
                     /locus_tag="VMU21_02840"
     CDS             complement(join(14930..15780,15780..16032))
                     /locus_tag="VMU21_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611840.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HUO76490.1"
                     /translation="MKKTRYSEEQIVGVLKEADAGIPVSEVCRKYGISDATYYNWKAK
                     YGGMAASDVKRLKQLEDENRRLKQLVADLTLDNQALKAVVNKKLLRPKARREAAVYMI
                     NCFGLSHRRASGLAGLSRNSLRYEAKPDRDEGLRGRMKELAEKRRRFGCRRLHVLLKR
                     EGLVRNHKRTERIYRQEKLSLKIRKRKKLASQGRVELPKAERPNQLWAMDFLQDALYN
                     GRRIRLLPIIDTYTKECFRIEVDTSIGGKRVATVLSQIAAFRGLPENIVVDNGPEFVS
                     NTLDAWAYERGIKLQFIRPGKPVDNAYMESFNGKFRDECLNQNWFASVDHARRVAEEW
                     RVDYNEERPHSALGDLTPREFLRLEEEKGVGIL"
     gene            complement(16078..16614)
                     /locus_tag="VMU21_02845"
     CDS             complement(16078..16614)
                     /locus_tag="VMU21_02845"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HUO76491.1"
                     /translation="MKELIPVEIIEKKILLIRGEKVMLDADLAELYGVEVKHLKRQVR
                     RNIGRFPADFMIQLSKEEYEFLRRHFGTLKRGEHSKYLPYAFTEQGVAMLSSVLNSER
                     AVRVNIEIMRAFVRLRQMLASNAELARKLEALEKKYDSQFKVVFDAIRQLMASPETKK
                     RKIGFHRDGPAPRKLCHL"
     gene            complement(16785..17678)
                     /locus_tag="VMU21_02850"
     CDS             complement(16785..17678)
                     /locus_tag="VMU21_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76492.1"
                     /translation="MSDQKRIILLIFIMALISLAIGIIGVSILYQTALHEEKDRLIET
                     VKSQARLMESVARFNSKYNKDVLYSSRQATISQIMNAHEHYEGLSKTGEFTIAEKEGN
                     EIVFLLTKQKKITQNVMRLPFDTRLAEPMRMALSGHSGQLVGLDYRGAEVLAAYEPVA
                     ELNLGIVAKIDLAEIRAPFIKAGIISGLIGMVLISLGALIFLKISNPLIKNLIESNRK
                     LEDALSQVKLLSGLLPICASCKKIRDDKGDWNHIESYISKHSEAQFSHGICPHCMEKL
                     YPDYYSQHVKEKLHEDKVNPE"
     gene            complement(17757..18047)
                     /locus_tag="VMU21_02855"
     CDS             complement(17757..18047)
                     /locus_tag="VMU21_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76493.1"
                     /translation="MKAKAATKKMTDEEYYDKKGVLGEIIEKDIAISLDEALAKDIVA
                     GKRKRKLQNVTIKLDPLYLMSIKKVATKKGIPKQTLVRQWVAEKIRRELKIA"
     gene            complement(18265..18480)
                     /locus_tag="VMU21_02860"
     CDS             complement(18265..18480)
                     /locus_tag="VMU21_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76494.1"
                     /translation="MPKAKGMTVDEIEQIVEQKMLEIIGDPDSGLHLKKKFRTQLEQR
                     LKKPSRRMSHEEVLKRFALRLNGIRKP"
     gene            18622..18828
                     /locus_tag="VMU21_02865"
     CDS             18622..18828
                     /locus_tag="VMU21_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HUO76495.1"
                     /translation="MDGTRRSDIRPGLRVSVVVKKDQRTGKLTEGIVRDILTNSTAHP
                     HGIKVRLESGEIGRVKGIFKVRES"
     gene            18891..19121
                     /locus_tag="VMU21_02870"
     CDS             18891..19121
                     /locus_tag="VMU21_02870"
                     /inference="COORDINATES: protein motif:HMM:NF021429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HUO76496.1"
                     /translation="MNEWQSNDPLHGITLEMILTRLVELHGWEKMGRIIRIRCFTHDP
                     SIKSSLQFLRKTPWARKKVEALYLKHKSGTRE"
     gene            complement(19601..19894)
                     /locus_tag="VMU21_02875"
     CDS             complement(19601..19894)
                     /locus_tag="VMU21_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76497.1"
                     /translation="MKKKSSHFSVEDIIRNEFKKPVSRGTIGKDGNGLLWAYGVVLKN
                     SRYCVEVITIDLEAHTVERIKDKESSYEQMADATAYITRLIKGILPVELPATQ"
     gene            complement(19894..20115)
                     /locus_tag="VMU21_02880"
     CDS             complement(19894..20115)
                     /locus_tag="VMU21_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76498.1"
                     /translation="MSTLDNLYNALTKKAQTANKDITREMVEDWVGDACPLDNQIALM
                     SMALGELYSGNYAAEEMVEDILQLTYLDN"
     gene            complement(20403..20726)
                     /locus_tag="VMU21_02885"
     CDS             complement(20403..20726)
                     /locus_tag="VMU21_02885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76499.1"
                     /translation="MHIKVDAEVINVSKGGMSIECTEKLERGKVYTVTLLWCGNVLDV
                     KCEVVWILDNGSAQKYRSGMNFIQIDKDNVSNFLTCLEEHVADARRLNPRMKVTGTRA
                     VLSYH"
     gene            complement(21151..21357)
                     /locus_tag="VMU21_02890"
     CDS             complement(21151..21357)
                     /locus_tag="VMU21_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76500.1"
                     /translation="MSEKCELLEKCGFFLNFKGNTEVVKQGWIRLFCEDKEKSEKCER
                     KRIRKQTGKPPADNMAPTGQIIGL"
     gene            complement(22075..22485)
                     /locus_tag="VMU21_02895"
     CDS             complement(22075..22485)
                     /locus_tag="VMU21_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HUO76501.1"
                     /translation="MIGTQRKSGHAVLIYDGECPVCRKTAAWIRENMRKDAFEMIPCQ
                     SGEARKRFPFIEQAVCMQAMQLILPDGEVLSAEKALPEIVKRLKRYSPAAALFEFPGA
                     GLLARSFYRWLADNRYHIAHVLFPDKKKHDRDNT"
BASE COUNT         5814 a         5559 c         5532 g         5889 t
ORIGIN      
        1 ctcagaagat cctcagggaa gcgcttagac ttttgaggag agggggcaaa cttctgatta
       61 tcgattggaa gaaagagcag acccctgaag gtcctcccct tgagataagg gtcacggaag
      121 aggccattga gtcccaaatg ctcaaggcgg gcttcagaga tatcgtaaag tatgctgtcc
      181 tcccgtatca tcatttcctg gttggagaaa aggtttgata ttgtttcccc attcatgtaa
      241 gcgcaggaat ctgcctgaag atttcagaat caacaccacc gcctttgaaa gagattatag
      301 gcttgagaca tcttaaacct tacccatacc atgtgaaaag gcctttgtcc tttttcagaa
      361 gccccaaaac ctttttggtt acacccgact ggcttgactt taggatggat gcccataaaa
      421 agggtgaaga ttagttctcg cttattgttc tcagggttgt cggcagagag gcaaacctca
      481 tgctcccggc atctgtgcgc attcatggaa gatccgcacc ttacagcgcc gacacctttc
      541 ggggtccagt ctcggcacaa tactccttat cgcctcgacc tttgagtgga acacgttttc
      601 ttctccaatg ctcttgttac agcctccccg cttcatgacg tctattacct cgcctttgag
      661 agaacagaga tatagctgcc ttccgttcac acgcagacga tgagcctcct ggttaagtgt
      721 ctgacatccc gcgacatcga tgaagtttat cccgcctcct acaatcagga tgtgcgcctg
      781 ttcagggtat ttttttgtta tttggtctaa ttgttccacc acatgattta ccgcaccaaa
      841 gaagattgaa ccgtcaatcc tgattatttt gagttgtgga cattcggaca gaggcttctt
      901 ctgcacattt atgaaagtct tcctcctgct ctcagggtca ggggcaaggg tcacaaaggt
      961 cggatgagat gttcggttaa gatagagcag cagcgaaaga agtaccccta cgtatatagc
     1021 aaattcaagc tccacaaata atgtagagag aaacgtcgca atgagtattc cggcctccgg
     1081 cttgcttgtc ctcacaattg aggcaacgtg gtgggtgtcg ataaggttat acgcaaccaa
     1141 aaacaggatt ccggccatcg aggctatcgg gagataggcg gtaagagggg ctatgagcag
     1201 taatattccg gtcagcgtta ttgccgcaaa gacagccgcc aatggtgtcc ttgcgcccga
     1261 ttcatagttc acccctgtgc gggtgaacga tccggatgag gcatagctcg aaaagaagct
     1321 cccaatgata ttggagagtc cctgaccgat gaactcctga ttgctatcaa tgagctgttc
     1381 agacttcgtt gctattgatc gggcaatgga gactgcttca gcaagcccaa gcatggcaac
     1441 agccagagca gggggcgcaa gctggcgcaa tgaagcagtc gtaatgtaag gatgtgatag
     1501 gggaggaaga tgaccgggaa gagagcccac gagtcgaata ccgtgctcgt tacccttcat
     1561 gagcagggcc atgacgctgc ctgcgatcat cgctatcagc attcccggcc acttgggctt
     1621 cagggtttta agaactactg cgataattaa cgtactgaga cctatggaaa ggacatagag
     1681 gttggtctcg gaaaggcgcg atagcatatc tgcccaaata tggatgaaag agtgttttat
     1741 tggtgcggtg atgcccagga tattgctgag ctggcttgtc gcaatgagta ttgaagcacc
     1801 tgcggtgaac cctacaacta cagagtgaga aacgaaattc accagggcac cgagacgggc
     1861 aactcccagc gccaattgga aaaatccggc gagaaaggtc agggtcaatg ccatctgaat
     1921 atactcagcg cttgaagggg gagccagggg gctgagtgcg gtgaagatca caatcgatat
     1981 tgccgtcgta ggaccggaga tgaggtgatg ggatgacccg aataaagcgg caataatagc
     2041 aggaactatt gcggaataaa gaccgtattc gggaggcagt cctgcaatga ttgcaaaagc
     2101 cactccctgc ggaagaacta taatcgcacc ggttatgcct gcgataatgt cagcccgcgt
     2161 actgcgacgg ctgactgcag gccaccactg caggggaggg aaaaacttct ttagggactc
     2221 tttttcttgt ccagatgcca cttcttcatt gttcttcatg gctctccgag attccttggt
     2281 aaatactgtc agctttttct tttctctcta tagagtatac cacctgccaa ttatttgcgg
     2341 atgtcaggga taattacgga tttctcaagg tctggcgaga gttaggatcg ttttgtcctt
     2401 gtttgacaaa agatgagtgg cgcgcctatg atttgcttaa tgaggttaat ttgatttact
     2461 ggcttaaggc tctatggcct gctcttttag tcttgacgct gagcatagtg ctttcccttt
     2521 tgcttggaga gttcgctttt ctaagaggtg acagggccat tgtaaagggt attttacgtt
     2581 tttcccggat tgctgtcttg tcgtctctga ctctttacct tgtgctcccc cttaatcgct
     2641 ggttgttgag acagatgaaa caccatcttc ttaaagttac agactccgca cctcctgcgt
     2701 tttccccgtt gaaaatctgg gtgtatcgtc ctttgcagtg tattggcacg agtctcctgt
     2761 tcgatacaaa acttatcact gcccttcagc tcgttgccgg ttcaacagtg tcagattccc
     2821 ttcttccaaa gggacgcttt aatatcgaat cttttttggc tacatccctc gttaccgcag
     2881 tgacatcttt gctgttagca agcatctgga ccctggacga tactggaata cgttacttca
     2941 atcggaaaga acatgagtta aaaatggccg gcaaattcgt aggtacgatt atgccgatcg
     3001 tttttggttt ttccggtgta agaggtcttt tggcgacatt tccgacgatt caggcaatga
     3061 tatatctctt ttatatattc attatcctct atccgccctt cactgtgctt agcgcattgc
     3121 attaccattt tgttaagagg aaattcgaaa ggttttctgg cataggggct ataagaaaag
     3181 ggatgatact agagaccaag agtggagatc tcaacagtta aataaataag agcgtaaaaa
     3241 agaaaatcga agctgaaccg aaacgagtat ccaaaccctg agctcccact gagcactgat
     3301 tcaaatctat tttaacgacc tcgttgcctt atctgcgaat cttctcaaaa aaaatataac
     3361 atttgacagc aggacatttt gtgcgatact tttcttatag gtaaggccat aacagggcgg
     3421 aacaaatggc ctgggcttaa aaggaactgc tgaggatttt caatggcggg aaaagcgatc
     3481 tggaaaggat atatcaattt cggggatgtc aatctccccg tgaagctgca taccgcaatc
     3541 aaggaagatc ggatccagtt tcacctcctt cacaagcgcg atcacctgaa attgcgccaa
     3601 cagatgatct gtgcgtttga aaaaaaaccg gtgccgacgg aggcacaggt caaagggttt
     3661 gaagtggagg aggggaaata tataatcgtc gatcctgagg aaatcgaaca gaccgccccg
     3721 gaagacagcc ggatgattga agtccgcgaa ttcgtcaaga ccgaacagat cgatcctctc
     3781 tttcttgagc gcgtctatta tctggaaccg gatgttcctt tgaaagggta tgacgctctc
     3841 gtcggtgctt tgaaggagat ggaggtagca ggtatctgca cctgggccat gagaaagagg
     3901 tactatctcg gagccttgca gacaggcggg aagattcttc gcctgaccac tctgcggtat
     3961 gccgacgaag ttatttccct aaaatctctc gaactaccgg acattcctct gtctgaaaaa
     4021 gaactgaaaa tcgggagtga tttgatcaat cgattgaccg cgccgtttca gcctcagaaa
     4081 ttcgaaaacg aacaccagaa aaagctccaa agtctgatcg agaaaaaagc ccgtggagaa
     4141 aaaatagcgg tcctgcgccc taggcgcctg aaacccaccg caccagataa gctgcttcag
     4201 gcccttgaag cgagcctgaa gaaagtcgcc taaatgaaaa cgcgcgatcc gatttcgaaa
     4261 ccatcgaagt cgtcttcaca cagggagaat gaagcggtca tgcagggaat atggagcggg
     4321 acgatcagtt tcagcctggt ggcgatcccg gtagacttgg ttaaggccgt cgatagcggc
     4381 cgagtctctt ttcgcatgct ccacagcaag gattactctc ccctccagag aagaatgttc
     4441 tgcccggaag aagaaacaat ggtccctccg gatgagatta tcagggggta cgaaatcgga
     4501 ccggacaaat acatcctgat atcggacgag gaattggagt ccgtgtcacc cgaacggagc
     4561 cgtacgatag agatcgtaga gttcatcgat atggatgaag tggatccgat ctattatgat
     4621 cacccgtatt atctcatccc ttcgaaaggg ggcgaaaagg cgtatcggct gctggtcgag
     4681 gtgatgcgcc ggacgcacaa ggcaggactc gcaaaattcg tgttgggaga gcgtgagtat
     4741 ctcgtagcgg tctatagctc ggagggagcg ctggccctca tcacacttca ttaccgcgaa
     4801 gaaatacttc ccgatgaaga tatctcaccg aagaaaggaa agatcgaaga cgaagagaaa
     4861 agccgtataa aacaaaacat caggaaaatg atggcggact ttgatccggg aaaatatgcg
     4921 gacaagcgcc ggaaagaaat catggacctc ctgaagaaga aggcgaaaga aaaagccccg
     4981 gtggaggccc ctgaaatgga aggggaagag ggagaaggtc cggccgatct ggtcgccgcg
     5041 ctcaaggaga gcatgcgtaa agtgaaaaag aaccggtgag ccgaaaagcc gtcgagatcg
     5101 ccggaagaag actctcttta tcgaaccttg aaaaggacct ctatccctcc tatggtttca
     5161 caaaggccca catcctggaa tactacctcg ggattgcgaa gttcatccta ccccatttga
     5221 aagaccgggc attgacctta aaacggtatc ccgaaggagt ggaaaaggat ttcttttttg
     5281 agaagcgttg tccttctcac catcctgaat gggtgaacac agcggaggtc agcccggatg
     5341 agggagagcg gatgacggtc tgcctggtca atgatctggc aacgctgatg tgggtggaga
     5401 accttgcatc tcttgagctc catgtgcccc tggccagagt cgattcccct gaaacgccag
     5461 attccatggt ctttgatctg gaccccggcg atcgggcaaa tattctaagt tgtgcgcggg
     5521 tggcactgat gcttcgggac cttctctccg ggttgcggct tacaagctat gtaaaaacct
     5581 ctggcaaaaa aggactccat gtatatgtcc ccctgaatcg caaagagacg acctttgaga
     5641 acaccaagac attttcaaag gccgtggcag agataatgca gaagaattat ccgaatttgg
     5701 tgactgcaaa aatggcaaag gaataccgga aagcaaaggt ctttatcaac tgggctcaaa
     5761 acgactcttc aaaaacgatg atctgcgtgt attccctgcg agcccgggaa aaaccggttg
     5821 tttcgtttcc ccttgcatgg aaggaactgg agaatttatc caagcagggc gacccggaaa
     5881 agctgcagat caagcattcg gaagcagtgg gaagggccga aaaacagggt gatctttttc
     5941 gagaggtgat cgtgaaaaaa cagaagcttc cttatctatg acgaaaacgc agatgggaac
     6001 aggccccttc gcccgaaagc ctgatttgac cagcagggga ttaaaggaat acgcatccaa
     6061 acggaagttc gggaaaacgc ccgaaccgaa accggggctg cgcactaagg ggggccatct
     6121 catttttgtt gtccataagc acgcggcccg ggccctccat tatgatctca gactcgagct
     6181 ggaaggggtg cttaagagct gggccgtgcc aaaggggccc acaatggatc cttccctgaa
     6241 aaggctggcc gtgatggttg aggaccatcc gtttgattac aaagactttg agggcgtcat
     6301 ccctgagggc aattacggcg caggaagcgt tatcatctgg gacaggggct tttatcatca
     6361 cccttccgca ggaggcagaa aagagagtga gaaacttctt ttggacggct taagaaaagg
     6421 aaatatgaag tttgtcctcg cgggagagaa gctgcagggt gaattcgcct tggtaaaaac
     6481 gggaagggac gggaagtcgt ggctgctttt gaagaaaaaa gatcgatatg caatccaggg
     6541 tgacattctc agggagaacc gctccgtagt ctccaataag accctggagg aagtcttcag
     6601 aaccaaaccg gagaaatctc ttagacaaaa aaaaataaac cagattcgtc ttcgggaagt
     6661 actggaaata gaggacctga aggatgcgcc ggtcaagcca atgccgtaca gcatagaacc
     6721 tatgctcgca accctgggga aagaaccgtt tgatcatccg gattggatct ttgaggtgaa
     6781 atgggacggg taccgggcaa ttgccgaaat ccgtgacgga gacgtttcgc tttattcccg
     6841 aaagcggaca ttgctaaacc aaaagttcct tcctcttgtg gagtcccttc acaaattagg
     6901 atttgaggcg gtgttagacg gcgaaattgt tgtagtggat gatcagggac atcctgattt
     6961 ccagatgctc cagaattacc agaaatccgg aaggggtcat ttgctctact acgtctttga
     7021 tcttttgtac tttcagggac atgacctgac gaacctgccc ctgctcagga gaaaagagct
     7081 tctcaaaaaa atccttccgt cttccccgaa aattaaattc agcgatcacg tatcgaaaga
     7141 gggcgtctct tttttcaatg tcgtcaaaga aaagggactt gaggggatca tcgccaaaca
     7201 ttcacaaagc gcctaccgga cgggaagaag gagccggcag tggctgaaga tgaagacgcg
     7261 gctcacgcaa gagggtgtga tcgcaggatt cacggaaccg agcgggggca gaaaatattt
     7321 aggctccctt gtcctgggcg tattcgagag gggtgatttg atttacatcg gccattcggg
     7381 aggcggattc tcggctaagg atttgaagga aatccacgaa aaattgcatc ccctgatcca
     7441 agaaaaatgc cccttcaaag tagagccgga gaccaatacc ctggtcacat gggtaaagcc
     7501 gaagctggtc tgcgaggttg ctttatccgg ctggactgaa gagggcatca tgaggcagcc
     7561 tgttttcctg cgactgcgcg aggataaagc cgcacgcgaa gtggtgggcg acaggccagg
     7621 gaaaagcaat aggtaaaaag ttgactaggg agcggccttg tcagccttct ccggtcattg
     7681 tatacttcaa ttatagccgg acaataaagt gctcccaggt aaataagatg agacacaaat
     7741 ctcttataaa gatttttatt cttattgcca taccgattac agtatttgcc gccaatccgg
     7801 tttcggtcac tatcaaagaa tggacccttc cgaccccgga ttccttccct catgatccgg
     7861 ctttgggacc tgacggctcg ctctggtata cgggcatggg gtccaatact cttggcaggc
     7921 tgaatccgaa aacaggcgag atcagggaat attcgttgaa gactgccaac tcgggccccc
     7981 atgggcttgt ggcagataga gaggggaatg tctggtttac tgccaatgcc aagggttata
     8041 tcggccggct caatccggta accggcaaga tcaccgaata tcaacttccg aaggcggcac
     8101 atgacccgca cactcctgtc tttgaccgga aaggcattct ctggtttact gtgcaggcag
     8161 gcaacatggt aggccggcta aaccccaaaa caggagacat taaggtcaaa ccattaccca
     8221 caccaaatgc ccggccctat gggatcgcgg tcaattcaaa aggcatcccc ttcttttgtg
     8281 aactcggcac caataaactt gcgcgcattg atccggatac tatgactatc agggaatatc
     8341 cccttcccag ggacgccagg ccaagacgcc tggcaataac ggctgacgac attatctatt
     8401 tcacggacta ctcgatcggg aaattaggtc gctttaaccc ctcagacggc aaaacagagg
     8461 aatggccctc gccaggcggc cgggattcca acccttatgc catagcaata actcctgaca
     8521 gtatggtctg gtacagcgaa tcgggcgtca ctcccaatac aattgtgagg ttcgatccga
     8581 agaccggatc attctcaagc tggccgatcc cctccggagg cggagtggtt cgaaatatgg
     8641 cagcaacccc ggaaggagat ctctatctgg catgcagcgg caaaaataaa gtggcaatag
     8701 tccatgtcac agaataagag agaagatgtt ctttacagat acgtgagcaa aggagaagct
     8761 gccatgagat tccttgtcct gaccgcagcg gttttcgcaa catcgtcatg ataggcgcca
     8821 tagcaggcga catcatcggc tctgtctatg aatggaaaaa cattaagacg acggaattcg
     8881 agatcttcag cagcccttgc cggtttaccg acgatacggt gctgaccatc gctgtcgcag
     8941 actgcatcct gaacggaaag cactatgcga cgacctacag ggaatacggc cggcgatatc
     9001 ctgatgccgg gtatggaggc ctctttaggg aatggctttt ttcagaccat ccgaggccct
     9061 ataacagctt cggcaacggc gcggcgatgc gggtcagccc tgtagggttt gccttcccct
     9121 cgctcgaagc agtcctggat gaagcaagga ggagcgccga ggtgacgcat aaccatccgg
     9181 aggggataaa gggggctcag gcaacggccg ccgcagtctt tctcgcgcgg gaaggagaga
     9241 gcaaagaggg gattcagagg tttatagaga agcggttcaa atacaatctc caccggaagc
     9301 ttgaggatat acggccggac tacaggttcg atgtgacctg ccaaggctcg gtccctgagg
     9361 caatcaccgc ctttctcgaa tccctggatt acgaggacgc ggtcagaaac gccatatccc
     9421 tcgggggcga tagcgatacc atcgcctgca tcacaggggg catcgcccag gcctattata
     9481 aaagcgtccc tcctttcatc gtcgagaagg tgcggggcat cctcccggat aagcttctga
     9541 agatcatgga tgattttaac aaggttttca gggcagccct gtgaagctgg cctttcccct
     9601 taccctttca acccttcgat aatcctcctt gcctgtttca taagatttac tgcctgtttt
     9661 atcctctgag ggtagtccat ggttcagatt ataaccaaat caggagattg gcataaaccc
     9721 ccgcctgagt tagcctccgc tgatcagctg gggcggaaag tggtgcgcga aaaaatctgg
     9781 ggaaaaacgg gtcattagtg gtataataaa caaccgatgc gaagcgattg cagcgtatat
     9841 ttatttcctc cccacatgcc ctccaatcgt tgtgttcctt ttattaaccg tcttgtcccc
     9901 gtctaaggag tttgtgtatg aatatcctgc tgctgtatcc ggaatatcct gataccttct
     9961 ggagtttttc ctacgcgctg aagttcacct cgaaaaaggc ggcctatcct cccctcggcc
    10021 ttctgactgt ggcagcaatg ctcccgcctg aatgggacaa aagacttgtg gacatgaatg
    10081 tccggaagct taaggacaag gacctcctgt gggccgacta tgtctttctc agcgccatgt
    10141 ctgtgcagaa ggaatccgtg aaagccgtgc tcgccagatg caaggaactg gatgtcaagg
    10201 tcgtggccgg cgggcccctt ttcagcaatg actacgaaga gttcggggat gtggaccacc
    10261 tggtgctcaa tgaggcggaa ataacgatgc agccctttct caacgaccta agggagggaa
    10321 ggccgaaacc gctctatgca tccgctgaaa aggcggacat gacgaaaact cccattcccc
    10381 tatgggagct gatagacatg cggcactatt cctccatgaa catccagtat tccaggggat
    10441 gcccctttga ctgcgaattc tgcaatatca catcgcttta cggccgggtc cccaggacaa
    10501 agtccgcagg gcagcttatc gcggaactgg agaagatata ctccctcgga tggagggagg
    10561 gggtcttttt tgtggacgac aacttcatcg gcaataaaaa gaagcttaag gatgatatcc
    10621 tcccgtcgct tatccagtgg atggaggagc ggaagcggcc tttctacttc tcgaccgagg
    10681 cctccatcaa tctggctgac gacgaggaac tcatgaagat gatggcgcag gcggagttcc
    10741 ttacggtctt tatcggcata gagacgccgc acgaggaaag cctgatcgag tgcgacaagg
    10801 tgcagaacag gaaccgcgac ctgatcgcca gtgtcaaaaa gatacaggac ttcgggctgg
    10861 aagtccaggc cggttttatt gtggggttcg accaggaccc tgcttcgatc ttcgagaaga
    10921 tgataacctt tatccaggag agcgggatcg tgaccgccat ggtggggctc ctgaacgcgc
    10981 cgcgtggcac aaaactctac gagcggctgg tcacagagaa caggctgtta aagactgcca
    11041 caggagacaa cacagacctc tccatgaact tcatccccaa gatgagttat gaaaagctta
    11101 tcaagggata caggcagatc atagagacca tttattcccc gaagcattat tacgcgaggg
    11161 tcaaacgctt catgaaggat ttccagccta tgcagaagaa ggtagtgcag ctgcggtaca
    11221 gctacttgaa ggccggattc aagtctgtgt tcatattggg gatgctcaga aaggagcgtt
    11281 tctactattg gaggcttgtc ttctggtccc tcttcaggaa gcccaaactc ctgcccatgg
    11341 ccgtgacctt ttccatctac ggtttccatt tcaggaagat tttcgagagg cacttcagga
    11401 ggatggaaag ggacaattgt gctcagacat gaccccttct gagcttttct ctatcgcacc
    11461 cgacgagtgg gttcccggtc tgttttaatc caaataaact gccccgtgtc tgctttaatg
    11521 aaggtctttc aaagcaaact aaagccttct ttgacagtga ttccctttct atgcgcgtac
    11581 cccgcagcca tgaatcgggt tgaatgtact atcgaaaaac ggccctgcgt atcgagcact
    11641 attactcctg cggcgccgcc tgtcctgagg agtcttttca gagagcggcg ggaagcagca
    11701 cggggagtca tgctgccaag gttcatgcat atctcttttg cgagggcaag cctgagtata
    11761 tattccccta tgccggtgca tgaaacagcg ccttgcgagt tttcggcgta aacgcctgcg
    11821 ccgatgatcg gcgtgtctcc gaccctgccc ggcagcatcg catggatgcc gccggtggac
    11881 gcgccggcag cgagattccc ttcactatcg agagctgctg cgcccacagt cgaaaagtaa
    11941 ttcttgaata tgcgaggcga gtcttttttc gttctcttta tcttctcaag cttctccaaa
    12001 gcccgcctgt caggtcccgg caaggggtca aggttatttg cctttgcgat cctctttgct
    12061 ccgatattcg tcagcatcgt attgggcagg tccattatca gccgggcgag tttaaccgga
    12121 ttttttaccc cttcaacgcc tatcaccgat cctgtcttta gtttcttccc ttccatcacc
    12181 gaggcatcca gcctcctgat cccgtcaagc tggaggttgc cgcctgttcc ggcattgaat
    12241 atccccgagt cttcgagcac ggttatcgcc ccgaccaccg catcaagggc cgtcctgccg
    12301 ttttccagta ttccatatcc atgcgagagg gaccaggaaa gggtcttcaa agccttcttc
    12361 gtcggccttc gttctcccgc tcccccatgc acaatcaaaa gcatgtactt cctcctcgct
    12421 tactcccgca gtccatgttc cagattataa ctgaatcagg agatgagcag aaagccgtct
    12481 gagtcagcct ccgctgatcc gctggggcgg aaagtagtgc aggaagccag gggaagcaat
    12541 gggggacatg cttacggatg aggtgtgata gtgacgatgg tgatctgggt ttcctcaggc
    12601 ccgtaatacc agaaaactct gtaagcggct ggagtggatt gctcagcata tgcctcaaaa
    12661 accttttctc ccctggggcc tttcaaagtt gtaaactcat gagtccgaag accgggatgc
    12721 cttggatttt tggaaaggaa gagaagggcc ttctttacgg ctttataccg cttggcaagt
    12781 cccttgtctg cctgaagcct atttagctgt tccctggcct cgtcggtagt gagtatctca
    12841 aacaagggac agtcctacag ttcgtcgggg ttgagtctcg tcactttacc cttagccgcc
    12901 tgttttagtc ccctttgaac gctatccagc gccttcttgt tcttgtaaag ccatgcttct
    12961 gaagagggta tgaggttcag aacagccttc actctgttaa actcctctat atcgataaca
    13021 gccgccattt tatggccttt tgtgtctgta aggaattgtt tgacttttat gtgatcatct
    13081 ttacgtcttg ctgctgtggg catatatacc tccttccggg aatgcttcat tataccacag
    13141 tgatcggcgt gtagaatcag atgggggaca ggcgtaaacc gtgaactggg tgatatggga
    13201 tagattgaca ggaggcatat acctgctata ttgggcttat gaataacctt gaggaggtgc
    13261 aacatgccaa atccatttgt gcacattgaa ttgcagacac aagacctgga aaaatcaaag
    13321 aagttctacg caggcatgtt cgactggaag ctggaagaat tcccgggcat ggactataca
    13381 ataatcgagg tcggtgaggg aacgggcgga gggatgatga agaagcctga tcccggcatt
    13441 ctggacaact ggcttccgta tatcatggtt gctgacattg cagcctcgac gaaaaaggcg
    13501 caggccctcg gggccacaat ttgcaaggag gtcaccgagg tcccggacat ggggtggttc
    13561 agcgtgatta cagatccgac cggcgcgaca tttgggctct ggcagccaaa ggcaatgaag
    13621 tagcccatta tcgttccttc caagctgttg ggtgaaggag gtgagtaaaa cttgacgagc
    13681 acatttatga ataagaaatc gcgtattgaa agtcgccgtt gtccgtgggt tgatctcagc
    13741 aaaccggagt atgtcgaata ccacgataag gagtggggtg ttccggtgtt tgacgacagg
    13801 accatctttg aattcctgac gctggaggcg gcccaggccg gtttgagctg gtacactgtt
    13861 ttacgcaaga gggatcatta tcgtcaggcc tacaggggct ttgaccctga aaaggtggcg
    13921 aggttcaatg cgtcagacat tgagcggctg atccaaaacc ccgggataat cagaaatcgc
    13981 ctgaagatcg aggcttctgt tcacaatgcc cgcaggtttc ttgaagtccg ggaggaacac
    14041 ggcagctttt caaactacat atggggcttt gtgaacaaca agccaaggat aaataaaata
    14101 aaaaagttga gtgattatgc ggcaacgagc aaggagtccg atgcattaag ccgcgatctg
    14161 aagaaaagag gatttaaatt tgtggggtcc acgacatgtt atgcacatat gcaggcaaca
    14221 gggatggtga acgatcattc gctcgattgc ttcaggagaa aagaaataat aagggactac
    14281 aagtcataag ctgagctgaa aattcgccaa cattatggta caaagctaaa ggattattca
    14341 tgcgcttgtt ttatagcgtg aggcatacca gtcgatgacc tgcctgatca ttttttgata
    14401 cgaagtatgg tgtttttgcg cctcttcctt aaaaaaatct atactcgatt ttcttaaagc
    14461 gatcgtcacc tttacatttt cttccttcag taccagttga tccggcgggg gaagaaaatc
    14521 cttcacaacc ctgagatcac ccatcggttc ttcactatat tttattttct tcttcatata
    14581 tcttcctccc ttttctccaa tatcccgcac cataaatgcg aataacatca tttctatacg
    14641 tgaacctcac agtcattatg ccttcaccaa ctctaccgat acaataaaac ctttcttctt
    14701 tggcgccatg gtgaatgtcc tcgacaatta cacgcttggg gtcaaaaaag gcatattgag
    14761 ccaatgaaaa agagacattg tgttttgcct ggttctccat gtctttctcc ttatcccatt
    14821 cgaaatgggt ctttttcatg gttttatgat actacggaat ggagataaag gcaatacaaa
    14881 agtatggcag tttatatgga cctgccccca tccgttgtgc caatcataat tagagtatcc
    14941 caacaccttt ttcctcttca agcctcagga attctcttgg cgtgagatcc ccaagagcgc
    15001 tgtgcggcct ctcctcgtta taatcaaccc gccactcctc tgctacccgt cgagcgtggt
    15061 cgacgctcgc aaaccagttc tgattcaaac actcgtccct gaacttcccg ttgaaactct
    15121 ccatataggc attatctacc ggcttcccgg gacggataaa ctgcagcttt atccctcgct
    15181 catacgccca tgcatccaat gtgttgctca caaattcagg tccgttgtcc acaacgatgt
    15241 tctcgggaag ccctctaaag gctgcgatct ggcttaggac cgtggcaaca cgtttcccgc
    15301 cgatcgatgt atccacctca atcctgaaac attccttcgt ataggtgtcg ataataggaa
    15361 gcagccttat cctcctgccg ttatataacg catcctgcag aaagtccatg gcccagagct
    15421 ggtttggccg ttctgccttc ggcagctcga ctctcccctg ggaggccagc ttcttcctct
    15481 tccgaatctt caaagacagc ttctcctgcc tgtatatcct ttctgtccgt ttgtggttcc
    15541 tcaccaatcc ctctctcttt aagagcacat gcagtcttct gcaaccaaac cgcctgcgct
    15601 tctcggcaag ctccttcatc cttccccgga gcccttcgtc cctgtcgggc ttcgcttcgt
    15661 accgcaagga gttcctgctt aatccagcaa gaccgctcgc ccgtctgtga ctcagcccaa
    15721 agcagttgat catatacacc gccgcttccc tgcgtgcctt gggccttaga agttttttgt
    15781 gactaccgcc ttcagcgcct ggttatcgag tgtgagatcg gccaccagct gctttaaccg
    15841 gcggttctca tcctccaact gcttgagccg cttcacatcg ctggctgcca ttcccccgta
    15901 ctttgccttc cagttgtaat aggtggcatc gctgatcccg tacttccggc aaacctctga
    15961 caccggaatc ccggcgtccg cttccttcag aacgccgacg atctgctcct ccgaatacct
    16021 cgtcttcttc atccttgcct ccttgtctga gtgtgctatt atacgcttac actctaatta
    16081 caagtggcac agttttcggg gggcaggtcc atccctgtga aaccctattt ttcttttctt
    16141 cgtctcgggc gaggccatca gttgccgaat ggcgtcaaag acaaccttaa actgagagtc
    16201 atacttcttt tcaagggcct caagtttacg ggccagttct gcattggagg caagcatctg
    16261 tctgagcctc acaaatgcac gcatgatctc aatgttgact cttaccgctc tttcgctatt
    16321 caaaacgctg gaaagcatgg caacgccttg ttcggtaaaa gcataaggca ggtatttaga
    16381 atgctcgcct ctttttaagg tgccaaaatg gcgccttaaa aattcatatt cttcttttga
    16441 taactggatc atgaaatctg ccggaaaacg gcctatattt cgtctgacct gtctcttcag
    16501 gtgcttcact tccactccgt aaagctctgc gagatcagca tctagcatca ccttttcacc
    16561 tcgaatcagt agaatcttct tttcaataat ctcaacggga attaattctt tcattcaccc
    16621 tccgtcttcc ttcttcgccc taactttctc aatcgcaaaa gtatatatct ttctcccagt
    16681 atttctcaat gatttcaatg agtaatgcat ggatggaacg aggagaagcg acataaggcc
    16741 gggacttcgt gtttcggtcg tattgaaaaa ggaccagcag ttctttactc cgggttgacc
    16801 ttatcttcgt gcaacttctc ttttacgtgt tgggaataat aatcaggata tagtttctcc
    16861 atacagtggg gacatatccc gtgactgaat tgtgcctcgc tgtgtttact tatataggat
    16921 tcaatgtggt tccaatctcc tttatcgtca cgaattttct tgcaagaggc acatataggt
    16981 aaaagaccac tcaggagctt cacttggctc agcgcatctt ccagctttct attgctttca
    17041 attaggttct ttatcaaagg gttactgatc ttgaggaaga ttagtgcacc cagggaaata
    17101 aggaccatgc cgattaatcc actgattatc ccggctttta tgaaaggcgc gcgaatctca
    17161 gcgagatcaa tttttgccac gatacccaga tttaattctg caaccggctc atacgctgcc
    17221 aacacttccg caccacggta atcaaggccg accaattgcc cggaatgccc ggacaatgcc
    17281 atccgcatcg gttcagccaa ccttgtatca aagggaagtc tcatcacgtt ttgtgttatt
    17341 tttttctgtt tagtcaagag aaacacaatt tcatttccct ccttctctgc aatggtaaat
    17401 tctcctgtct tgcttaaccc ttcatagtgc tcatgagcat tcattatttg gctgattgtc
    17461 gcttgcctcg aactgtagag aacatcttta ttatatttag aattaaatcg ggcaaccgat
    17521 tccattaaac gtgcctggct cttgacggtt tctataagac ggtctttttc ttcatgaagc
    17581 gccgtttggt aaaggatgct gactccgata atacctatag ccagagatat gagtgccata
    17641 atgaagatta agaggataat tcttttctga tcgctcatct gccttctctc tttcggtcgt
    17701 attgaagccg tgaatcgtga atagtaaata tagatacggg ctgaagagcc gccgccttac
    17761 gcgattttaa gctctctccg aatcttttcg gcgacccatt gccgcaccag cgtttgtttc
    17821 ggaatcccct ttttcgttgc cacctttttt atcgacatga gataaagtgg gtcgagcttg
    17881 atggtgacat tttgcagctt acgcttccgt tttcccgcta caatatcctt cgcaagcgcc
    17941 tcatcgagac tgatcgcaat gtccttctcg atgatctcgc caaggacgcc cttcttatca
    18001 tagtattcct cgtctgtcat tttcttcgtt gccgctttag ccttcatggt ctgtatatct
    18061 ctcttctatg cccaacagct ctaatgacga tcacatcttt ccctatttcg tatatcactt
    18121 tccagtcgcc gactctcagt ttaaacaagc cggccatgtc agcagacagt ggttcaggag
    18181 tgacggcgtc gaaatattgg gaaaaccacg agattttatg caggatacgt tttactatcg
    18241 gtctgtcaat cttttcaaga tcgtctaagg cttccttatc ccattcaacc ttaaggcaaa
    18301 tctcttcagg acctcttcgt gggacattct cctcgaaggc ttcttaagtc tctgttccag
    18361 ttgagtcctg aatttcttct ttaaatggag acctgaatca gggtcgccga taatttcgag
    18421 catcttctgc tcgactatct gttctatctc gtccactgtc atcccttttg ccttgggcat
    18481 actgcacctc cgttctgcgt ttattatatt cccaaaaaga ctttattgac aaccctgaca
    18541 gggatcaggg tcatctgcat catcccttcg actcgccggt gtataattag agaaggattt
    18601 cacacggcag gagagtaatg catggatgga acgagaagga gcgacataag accaggactt
    18661 cgtgtttcgg tcgtagtgaa aaaggaccag cggacaggga aactgacaga ggggatcgta
    18721 agagacattc tgaccaattc aacggcccat ccgcacggga tcaaggtccg ccttgaaagc
    18781 ggcgagatag gacgcgtaaa ggggattttt aaagtccgtg aatcgtgaat agtgaaacgt
    18841 gaatagtaaa taccgatgaa agcagaagta atccattatc aggctaaagc atgaatgagt
    18901 ggcaatccaa cgatcctctt cacggcatca ctctggaaat gattctgacc cggctggtag
    18961 aacttcatgg ctgggagaag atgggaagaa tcatccgaat caggtgtttt actcatgacc
    19021 cgagcatcaa atccagcctg caatttctga gaaagacccc ctgggccaga aaaaaggtcg
    19081 aagccctgta cctgaagcac aaaagcggga ccagagagta acccgccgaa cggatggaat
    19141 tggggtcaag ccttacttat cgtatcccac ttcacctata tcttatcttc aaaacaagaa
    19201 ttgtgaatgc cagagtaaag ctgacaatat tggcaataat aatcggcatt gaatttatcg
    19261 caaaaccata gaatgtccat aagagaagcc ccatacaaga tgtagagaac atccctgctg
    19321 aaatatcttt ggttgatttt gacctccatg tcttgacaac ctgtgggagc agagcaattg
    19381 ttgtcagcgt gccagccatt attcctaaga gtgttattcc gttcatcgca taatagttta
    19441 ccatttgtgg gattgcctag agggtaatgg gaaactttct tcaatagttc agcccatacc
    19501 ctcagggaga aggcagggga tgatgccgta aatgatgatt tcttgtgcca tatggcacct
    19561 ccagttagac gttcatacca cagccgagtc taatcctgtt ttattgagtc gctggaagtt
    19621 ccactggaag gattcccttt atcaatcgtg ttatgtatgc tgttgcatct gccatttgct
    19681 catatgagct ttctttgtcc tttatccgtt ccaccgtatg agcctccagg tctatcgtga
    19741 tcacttcaac acagtagcgg gagtttttca gaacaacacc gtaagcccac agcaaaccat
    19801 tgccgtcctt gccgatagtt cccctgctga caggtttctt gaactcattc ctgattatgt
    19861 cctcaaccga gaaatgcgat gatttctttt tcattagtta tccaaatatg tcaactgaag
    19921 aatatcctca accatttctt ctgcagcata gtttccgcta tagagttcac caagtgccat
    19981 agacatcaag gctatttggt tatcaagagg acatgcatcc cccacccaat cttccaccat
    20041 ttcccttgta atgtccttgt ttgctgtctg cgcctttttc gtcaaggcat tatataggtt
    20101 atccaatgta gacatttgtt acccctttat ctttcatgct ttattatatc atgcggaggt
    20161 ggtgaggctt tccaagaatg cttaccggaa tgagtacatt gccttgactt gtctgaattt
    20221 tgtgataagt agtaattaag aaacgcgtat tccggtctag tttaatcgta tcacttcgag
    20281 aaagttcttt atgtcgtgtt caccgaccaa caaagagttt caagacatcg caacttgtgc
    20341 gaattaccat ttgaaagaaa caagaaagca gcaattgaga ccttattctg agtcattcaa
    20401 acctagtgat aactcaacac cgcccgtgta cctgtcacct tcatcctggg attaagtctt
    20461 ctggcatcgg ccacgtgttc ctctagacac gtcagaaagt tgctgacatt gtctttgtca
    20521 atctgaataa aattcattcc tgaccgatat ttttgagcag agccattgtc aagtatccag
    20581 acaacctcgc acttcacatc taagacattg ccacaccaaa gtagcgtgac ggtgtaaacc
    20641 tttcctcttt cgagcttttc ggtacactca atggacatgc cccctttgct cacattgata
    20701 acttctgcat ccaccttgat atgcatcaat agctaccggg tttagaatcc ggcgtggaat
    20761 tcctccatgt ctgcccatgt atgaatcttg atgccagcaa aagcacctac ggtggcggct
    20821 ccgactcgta taaggaaatg attggatatg agatgtattg catccaacct agacttaata
    20881 gctttaataa acttctgcca cgcctctcgg gcaccgctgt cgtaatcggt catgcgggtg
    20941 cactcccaga ccatcacgag tttgtcagac gacgagtcga tcatggatgt gatctttcta
    21001 accgcatttt gggcgttatc ctctgaaagg tgtccgtcga aatgcagaac aagacaacgt
    21061 cgggatttaa tgtcgcacca ctcaatattt ggattcataa ttttccctta aggcggagcg
    21121 ctttgttagc caacatcatt gcaattggcg ctacaacccg attatttgtc cagtgggagc
    21181 catattgtct gcaggtggtt tgcctgtctg ttttcgtatt ctctttctct cgcacttctc
    21241 tgacttctct ttgtcttcac agaacagcct aatccaacct tgtttgacaa cttcagtatt
    21301 tcctttgaaa ttcaagaaga atccacactt ttccagtaac tcgcatttct ctgacatgat
    21361 cgtctccttt cgtgtgatta tttttattta cttcattgga gaatatttgc aaatatcatg
    21421 ctcatgctca gtttctcatt ctcagacgct caatagttta tataatgaca ttatttcaag
    21481 gagttactaa ttccaatatc ggattcgctt gtggcgtaaa agaagtgaca gagctgatga
    21541 gatgtctgaa aataaaacgg tatgacctga aatcctacaa agattaacct gatgaaaagt
    21601 ttgtcgacct agtgaacgtc ccttaaagca tcagggatat caatcaataa accgtcgtga
    21661 aaattaacaa gaaaggcgaa cctggaatcg ttgatttaca cgagaaactt gcagaactat
    21721 attagatagg aaagatgcgg acttcaggag ggaagtgatt attgtaagag gacagtgata
    21781 gggaattaat tttagggagg aggaaacagg ggtacaataa ctcaccctgt ttgacttggg
    21841 gcgcctcggc ctcatccagt cctcccctta gtttaagttt cctctacaca taaccacttt
    21901 gtcaacttga agttttgata atgaggtgtt ttattgctta tgatttgatt ttcggataca
    21961 aagaacgttg atttccaatg tactctttac tcaaagttag tatatcaggc gtgttgtgta
    22021 cagggagctt aggctgaagg ctgaagttct cccattatcc aaataaaagc tgtttcaggt
    22081 gttgtccctg tcatgtttct ttttgtcagg gaacaggacg tgtgcgatgt gatatctgtt
    22141 gtccgcaagc catcgataga atgaacgggc aaggagccca gcgccgggga actcaaagag
    22201 tgccgcagcc gggctgtacc gcttcaggcg tttcacgatc tcaggcaggg ccttttcagc
    22261 gctcagcacc tccccgtcag gaaggataag ctgcattgcc tgcatgcaga cggcctgctc
    22321 gataaagggg aaccttttcc gtgcctcccc tgactggcag gggatcatct cgaaggcgtc
    22381 cttgcgcata ttctccctga tccatgccgc ggttttccgg cagacagggc actctccgtc
    22441 atagatgagc actgcgtggc ctgatttcct ttgcgttccg atcacgacgt atacgctcct
    22501 gctccgattg taccagacag gaaatgaccg cgcattcttt gctgccggga ggcgatatct
    22561 tccggtctgt tgccctgcct gcggagaatg ttctttgcct tgccttgact tgtctgaatt
    22621 ttgtgataag tagtaattaa gaaatgcgta gcggatgtgc ttttcttaag aaaggaggac
    22681 gttatggtta agacagcgct gtttgttcga ctggaggcaa aatccgggaa ggaggctgaa
    22741 gtggagaagt ttcttcgcgg gggccttgca atcgtcatgg aggagccggt cacg
//
LOCUS       DATKOU010000061         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3921891, whole genome shotgun sequence.
ACCESSION   DATKOU010000061 DATKOU010000000
VERSION     DATKOU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3921891"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..649
                     /gene="pilO"
                     /locus_tag="VMU21_02900"
     CDS             26..649
                     /gene="pilO"
                     /locus_tag="VMU21_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HUO76502.1"
                     /translation="MALKLNIKMDTLPLAVKVLIAFLPAIVISALVIILLILPRHKEI
                     QRLNVEITKQENDIAKDQVKAAKLSTLKGENVKLRMRLDELKLQLPEEREVSGLLKQV
                     SDLGIKSGLKIVSWRPEQKKDHPSGTIYEIPVAVELSGSYHNLGIFFSRLTKLNRIVN
                     ISDIKMGGPQPQRSEAIVRISFKATTFSAISDKEAAAKTAAAKTKNQ"
     gene            646..1218
                     /locus_tag="VMU21_02905"
     CDS             646..1218
                     /locus_tag="VMU21_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HUO76503.1"
                     /translation="MMKAAHTSRLRLKEFLLLSVTVTLTVALTVACEKEKPKGIQKQA
                     QKVQPAEPPKKGEAASQKPEEKKVEAEAYSYSPQGRRDPFLSIIEASKKEQESEKKKR
                     SLRPSEMYDVTEIRVIAIAKDKNRSYAMVQLPDKKYFTVKEGMTLGLNNGKVISIDAG
                     SVMVREYIKNYKGEIQSKDTTLRLRKEEGE"
     gene            1215..3050
                     /gene="pilQ"
                     /locus_tag="VMU21_02910"
     CDS             1215..3050
                     /gene="pilQ"
                     /locus_tag="VMU21_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HUO76504.1"
                     /translation="MRRNTLNIILSMVVLTVLCVAGAPHAGYAKGESPGVTAITGINV
                     MDEALEIKGDRPFVYTIYKSSDPYKVMVEIPDADIGAFRNVIKSDSAGISEVTPSQID
                     VPKPMAKIEILLQSPSGVDSQYHNNSLVVRMKKDGGEQRSEPMKLAALQTEEKGSLPA
                     DTAKPNVQEPQPPRMDEEKMALKEPAEGGNNKDRISLDFQDADVVPIFRLLADISGKN
                     IVVSPDVRGKLTMKLINTPWEQALDLILKTTIPPLDKDVEGNVIRIATAESMRKEREA
                     QAAERRKLVAEEPLETKTFDINYADVSVVEKAIKDARILSPRGSLSVDKRTSSMTVND
                     VPAVFPQVKDLLSTLDKSTPQVMIEARVVEVNTTDSRDLGIQWGVTVKGTNTLSLLTG
                     LTTLNKGAFAGAPFMVDLPAGSVAAGSGTGFTFGVLSPDKTFGLDLQLSALQKIGNTK
                     IISNPRIVTTDNEKALIMQGTSEPFPKLTTEGTISTEYKDVVLSTEVTPHITPTGAIS
                     MKINVKKEDILGTVNIGGSQVPRTSKIESNTLVLVQNGETIVIGGVYKKTTTENDSGI
                     PGLMKIPILGWLFKNNTVSETVSELLIFLTPRVVEQPGGERAAKL"
     gene            3104..3748
                     /locus_tag="VMU21_02915"
     CDS             3104..3748
                     /locus_tag="VMU21_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76505.1"
                     /translation="MKRGNKVMLLTLFCVLSAVFFCGCGGSGPGSPGSTECENFGLIC
                     SVTITPVYLGSNTDNVDAAEDVCDPGPPAVFEKFTDHGANVAVSLQLLNPTTTLTPSS
                     LFIESYTIEYRRSNDSIGSPSIQSDTRFISFSVTPPTGTDISTVTQSLIFVDIPRKDK
                     YYQDMLSGVFGATTGSPSFINNYTAIYTIQGQINGTKVTIIGTKSFSIGDYNNC"
     gene            3770..>4092
                     /locus_tag="VMU21_02920"
     CDS             3770..>4092
                     /locus_tag="VMU21_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76506.1"
                     /translation="MKKIILLLGIVALSFGTFGCGGGGAGSAALPPGENSGVPSVVQL
                     LPSQFIAQTNSSITLHAKVLDGNGTPVVGVPVTFTNLSEPFGVIKRTLAKLRIIKSVG
                     ILSATV"
BASE COUNT         1134 a          984 c         1124 g          850 t
ORIGIN      
        1 ttgaccttta gagtggcggc ataagatggc gctaaaactc aatataaaaa tggataccct
       61 gcccctggcg gtaaaggtgc tcatcgcctt tcttcctgcc attgtcattt ccgcgctcgt
      121 gatcatactc ctgatactcc cgcggcacaa ggagatccaa aggctcaatg tggaaatcac
      181 gaaacaggag aatgacattg caaaggacca ggtgaaggca gcgaagcttt ccacgttgaa
      241 gggagagaac gtgaagctcc gaatgaggct ggatgagctg aagttgcagc tccccgaaga
      301 acgggaggta tcggggcttc tgaagcaggt ttcggacctg ggaataaaat caggtttgaa
      361 gatcgtttca tggaggcctg agcagaagaa ggaccatccc agcggaacaa tatacgagat
      421 acccgttgca gtggaactct cgggaagtta ccacaacctc ggcatctttt tcagcagact
      481 gacgaaactg aacaggattg tgaacatctc tgatataaaa atgggaggcc cccagccgca
      541 gaggagcgag gccatcgtca ggatcagctt taaggcaacc accttctcgg caatatctga
      601 caaggaggcg gcagcaaaga ctgctgcagc aaagacgaag aaccaatgat gaaagctgct
      661 cacacatcaa ggctgaggtt gaaggaattc ttactgttaa gcgtaaccgt aacattaacc
      721 gtagccttaa ccgttgcttg tgagaaagag aagccgaagg gaatccagaa acaggcgcaa
      781 aaggtccagc ccgcggaacc tcccaagaag ggggaagcgg cctcccagaa acctgaagag
      841 aaaaaagttg aggcagaggc atactcatac agcccgcagg ggaggaggga ccctttcctt
      901 tctatcatag aggcctctaa gaaggaacag gagtcggaga aaaagaaaag gagcctcagg
      961 ccttcggaaa tgtatgatgt taccgagata agggtaattg ccattgcgaa ggacaaaaac
     1021 aggtcctatg ccatggtcca attgcctgac aagaaatatt ttaccgtgaa agagggcatg
     1081 accctgggcc ttaataacgg aaaggttatc agtattgacg cgggaagcgt catggtgagg
     1141 gagtacatta agaattataa gggagaaatt caatcgaagg acacaacttt gagactccgt
     1201 aaggaggagg gagaatgaga aggaatacac taaatatcat cttatcaatg gtggttttaa
     1261 ccgtcttgtg cgtggcgggg gctccccatg caggctatgc aaagggagag tcgccgggtg
     1321 tgaccgccat caccgggatt aatgtgatgg acgaagccct ggagataaag ggggacagac
     1381 cttttgtgta taccatctat aagtcgtctg acccttacaa ggtcatggtg gagatacccg
     1441 acgcggatat cggcgccttc aggaatgtca ttaagtcgga cagtgcgggg atttccgagg
     1501 ttaccccgtc gcagatcgat gtccccaagc ctatggcaaa gatagagatt ctcctgcaat
     1561 ccccttccgg tgttgattcc cagtatcaca ataacagcct tgtggtgagg atgaaaaagg
     1621 acggcgggga gcagaggtcg gaaccaatga agctggcggc cctgcagaca gaggaaaaag
     1681 gctcgctgcc ggctgacact gccaagccaa atgttcagga acctcagccg ccacggatgg
     1741 atgaagagaa gatggcgcta aaagaacccg ctgagggtgg aaataacaag gacaggatat
     1801 cccttgattt tcaggatgcg gacgttgttc ccatattcag gcttctggct gatataagcg
     1861 gaaagaacat tgtggtgagc cctgatgtga ggggtaagct caccatgaaa ctgatcaata
     1921 ctccatggga gcaggccctt gaccttatcc tcaaaacaac gatccctccc ctggacaagg
     1981 atgtggaggg caatgtcata aggatagcca ccgcagagtc catgagaaaa gaaagagagg
     2041 ctcaggcagc ggagagaagg aaactggtgg ccgaggagcc cctagagacg aagacattcg
     2101 acataaacta tgctgatgtc agcgtggtgg aaaaggcgat caaggacgca aggatactct
     2161 ctcccagggg cagcctgagc gtggacaaga ggacaagctc catgaccgta aatgatgtgc
     2221 cggccgtatt cccccaagtg aaagatctcc tgtccacact ggataaatcc acaccccagg
     2281 tgatgataga ggcacgggtc gtggaggtaa acacaaccga ttccagggat ttgggcatac
     2341 agtggggggt gaccgtcaag gggaccaata ccctttcact tttgaccgga cttaccaccc
     2401 tcaataaagg ggcgttcgca ggtgccccct tcatggttga cctgccagcc ggctccgtgg
     2461 ctgccggatc aggcacaggc tttacctttg gcgtcctgag ccctgacaaa accttcggcc
     2521 ttgaccttca gctttctgcc cttcagaaga tcggcaatac caagataatt tccaacccga
     2581 ggatcgtgac cacggacaac gaaaaggcgc tcatcatgca gggcacgagc gagcccttcc
     2641 ccaaattgac cacagagggg acgatatcca cagagtacaa ggatgtggtg ctcagcactg
     2701 aggtgacgcc ccatattacc cctacaggcg ccatcagcat gaagatcaat gtgaagaagg
     2761 aggatatcct gggcaccgtg aatatcggtg gttcacaggt cccgaggacc tccaaaatag
     2821 agagtaacac gcttgttctt gtacagaacg gagagacgat tgttatcggg ggagtctaca
     2881 aaaagaccac aacagaaaat gattcaggca ttccgggcct catgaagata cccattctcg
     2941 gatggctgtt caagaacaac accgtttcgg aaactgtgag cgaacttctc atattcctta
     3001 ccccgcgcgt tgtggaacag ccgggggggg aaagggcagc gaaactctaa atgattggac
     3061 tgcggaagat aatttatgat aaaggatggt taagggagga gagatgaaga gaggaaataa
     3121 agtaatgctg cttacactgt tctgtgtgct gtctgccgtg tttttctgtg gctgcggtgg
     3181 cagcggtccg gggtcgcccg gcagcaccga gtgcgagaac ttcggcctca tctgctcagt
     3241 tacaataact ccggtttatc tgggcagcaa taccgataat gtggacgcgg ccgaggatgt
     3301 ttgtgaccca ggtcctcccg cagtattcga gaagtttacg gaccacggcg cgaacgtagc
     3361 ggtcagcctg cagctcctaa accctactac tactttgaca ccgtcgtctc tcttcattga
     3421 gagctacacc attgagtaca ggcgctcaaa cgactccatc gggtcgccgt ccatccaatc
     3481 ggacacgagg ttcatttcat tctccgtcac ccctccaacg ggcaccgata tcagcacggt
     3541 gacgcagagc ctgatttttg ttgatatccc gaggaaggat aaatattatc aggacatgct
     3601 gagcggcgtg ttcggcgcta ccacgggatc accgtctttt attaacaatt atacggcaat
     3661 ctatacaatc cagggtcaga tcaatggcac caaggtcact attatcggta cgaagagttt
     3721 ttcgatcggc gactataata attgctaata agataaactg gagggtgtaa tgaaaaagat
     3781 cattcttttg ctggggatag tggctctgtc cttcggcaca ttcggttgcg gcgggggagg
     3841 agccggctct gcggctctgc cgcctggaga gaattcgggt gttccctctg tagtgcaatt
     3901 gcttccttcg caatttatcg cccagacaaa ttcgagtatc acgctgcatg ccaaagtcct
     3961 tgacggcaac ggtacgcccg tcgtgggcgt gcccgtgacc ttcacaaacc tttccgagcc
     4021 ctttggtgta atcaagagga ccctcgcgaa actaaggatc ataaaatccg taggcatact
     4081 cagcgccaca gt
//
LOCUS       DATKOU010000062         5236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_76028, whole genome shotgun sequence.
ACCESSION   DATKOU010000062 DATKOU010000000
VERSION     DATKOU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76028"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1557
                     /gene="thrC"
                     /locus_tag="VMU21_02925"
     CDS             322..1557
                     /gene="thrC"
                     /locus_tag="VMU21_02925"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HUO76507.1"
                     /translation="MGYVNGLKCRECGREYPIDPIYVCEFCFGPLEVVYDYKKIKRVL
                     KRKNIEKREGNLWRYKELLPIDGDPQVGLESGYTPLIKADNLAKELGMKELYIKDDTV
                     AHPTLSFKDRVVAVALTKAKEFGFDTVACASTGNLAHSVSAHGAKAGFKRFVFIPASL
                     EPSKIVASLVYEPNVVAVDGNYDEVNRLCSEIANKYKWAFVNINIRPFYAEGSKTQGF
                     EIIEQLGWKAPDNVVVPCASGSLLTKVWKSFKEFKAIGIIKELETKVFAAQATGCAPI
                     SVALKQGTDVIRPVKPNTIAKSLAIGNPADGFYAIQAVKATGGYAEDASDEEIIEGMK
                     MLARTEGIFAETAGGVTLACAQKLIESGKIPRDETTVLCITGNGLKTQEALNGHMESP
                     HYIKPNLASFEEILQKIGH"
     gene            1607..1885
                     /locus_tag="VMU21_02930"
     CDS             1607..1885
                     /locus_tag="VMU21_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUO76508.1"
                     /translation="MAVKVRIPTPLQRLTQGKEEVEGVPGSVMQLVTDLDKRFPGLGE
                     RISEGGKIRRFVNIYVNEEDIRFLKAEDTEVKDGDEVSIVPAIAGGMR"
     gene            1941..2177
                     /locus_tag="VMU21_02935"
     CDS             1941..2177
                     /locus_tag="VMU21_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HUO76509.1"
                     /translation="MKRRVKLTFPPQLIREPVIFTMAKRFEVMPNIRRARVTETVGEM
                     VLELEGTEENLGKGIQSLRDQGVDVELVEGDIIE"
     gene            2304..3518
                     /locus_tag="VMU21_02940"
     CDS             2304..3518
                     /locus_tag="VMU21_02940"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HUO76510.1"
                     /translation="MKYIVIVGDGMADRPLKELGGKTPLQKAFTPNMDRLAKEGIIGS
                     VRTVPRGLHPGSDIANLSILGYDPKLFYTGRAPLEAASMGVELRETDVAYRCNLVTLR
                     YNKERTRAVMEDYSGGHVSTAEAALLIREIDETLGTSDIKFYPGVSYRHLMVWKDGEL
                     RVECVPPHDILGKDICDYLPTGYREEVVRKMMMDSVGILSSHPVNKERLAAGKKPANS
                     IWLWGQGKKPRIPRFKEKYNLDGALVSAVDLTKGLGVYAGFEILRVPGVTGYLDTNYT
                     GKAEYSLNALENVDLVYVHVEAPDEAGHSGNYVDKVKAIEDFDALVVGTIIRGAKHFQ
                     AYRILLMPDHATPIEAKTHTDEPIPFVIYDSRAIRNNEGATFNESIVGRKDIMVVEEG
                     YKLMDYFIKGTL"
     gene            3693..5183
                     /locus_tag="VMU21_02945"
     CDS             3693..5183
                     /locus_tag="VMU21_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUO76511.1"
                     /translation="MNDEHIEKKSPDAKKAELHKDREESFASLLKRSSMGERLYPGQK
                     VRAKVVSISGDLVYIDLGGKSEGAVDLAEFVNKEGLLSIREGDEIEASFVTVQDGLMK
                     LTTLVGGYSALTLNSIRDAYEGGISVNGEVKQEIKGGFEVSVGGVRCFCPFSHIDLRG
                     SREGSAYVGQTFPFKVLEYGEEGGKIVLSRRVLLEEEKKARREKFKERLTVGMDVDAE
                     VKSIQKFGAFVDLGGIEGLIPASEISWDRTVNPNDVLSIGKNITAKIISLDWEHDRLT
                     LSLKAALPDPWASVADKYPVDSRVSGSIARLLPFGAFVRLEPGIEGLIHISNLGAGRR
                     INHPKEVVEVGQLVEVYVLAVDQQNRKVSLSMQPKAEPVKVVLPAAGEILDGIVEKVM
                     PYGIFLKMNNGIKGLIPSAEMGTPSGTDHRRMFPPGTEMQVAVIDVDTIHNKVRLSRK
                     AVLEKAAHDEFAHYTESAKKSAESSRGLGSLGDILKARLEEKKNRG"
BASE COUNT         1446 a         1065 c         1523 g         1202 t
ORIGIN      
        1 ctttttgaaa aggcacgaaa gaaaatcacc taatgccaaa aaggaagaat gagcagtttt
       61 tttaggagat tacagaaaga gttataggta agcgcggaaa gcagatcatt tttccgcacg
      121 atggctgcac ccttggcggg gaaaattact tagatagtag ctacactcag tgcctattcg
      181 ccggagaatc tatatgaatc gttcataagg aatgctattc tccgtcgcta tttaattttt
      241 ggcacaaatt agagtaaaat acacatctat tgaatttcaa aaggtttctt gagaacttct
      301 caggctcgac aggaggtgtc tatggggtat gtaaatggtc ttaagtgcag agagtgcggc
      361 agggagtatc ccattgatcc catatatgtc tgcgagttct gtttcggtcc gctggaagtt
      421 gtgtatgact ataagaaaat aaagcgagta ctgaagagga aaaacattga gaagagggaa
      481 gggaatctct ggcgctacaa ggaactcctt cccatagacg gagaccccca ggtgggtctg
      541 gaatcagggt acaccccgct tatcaaggca gataacctgg ccaaagaact gggaatgaaa
      601 gaattatata ttaaggatga taccgttgcc catcccacgc tttcgtttaa agacagggtt
      661 gtggcggttg ccctcaccaa ggcaaaggag tttgggttcg atacggttgc ctgcgcatcg
      721 acaggaaacc ttgcccactc tgtttctgcc cacggggcaa aggcagggtt caagaggttt
      781 gtgtttatcc ctgcctcact ggagccgagc aagattgtgg catcccttgt ctatgagccc
      841 aatgtcgtgg ctgttgatgg gaattatgac gaggtgaaca ggctttgcag cgagatcgca
      901 aacaaatata agtgggcatt tgtgaatatc aatatcaggc cgttttatgc cgagggctcc
      961 aagacgcagg ggttcgagat tattgaacag ctgggatgga aagcccctga caatgtggtg
     1021 gttccctgtg ctagcggttc tcttctcaca aaggtctgga agtcctttaa ggaattcaag
     1081 gccataggaa taataaaaga gctcgagacc aaagtgtttg ctgctcaggc cacgggatgt
     1141 gctcccatat ccgttgcact gaagcagggc accgatgtga taaggccagt gaaaccgaat
     1201 accattgcca aatcccttgc cattggcaac ccggcagacg gattttacgc aatacaagcc
     1261 gttaaggcga cgggtggata cgctgaggac gcttctgacg aggagatcat agagggcatg
     1321 aaaatgctcg cgaggactga ggggattttt gcagagacag caggtggcgt taccctggca
     1381 tgtgcacaga aacttatcga atcaggcaag attcccaggg acgagacaac ggtcctctgt
     1441 attaccggca acggactcaa gacccaggag gcgttgaacg gccatatgga atcaccccat
     1501 tatataaaac cgaatcttgc atcttttgag gaaatacttc aaaaaatcgg gcattagttc
     1561 ccgctgcaaa gtggagactg cggacatttg aacaggaggt ggtataatgg cagtaaaggt
     1621 aaggatccct acaccgctgc agagattaac gcagggaaaa gaagaagttg aaggagtacc
     1681 gggcagtgta atgcagctcg ttaccgacct ggacaagagg ttccccggcc ttggggagag
     1741 gatctccgaa gggggcaaga tacggaggtt tgtgaacatc tatgtgaatg aggaggatat
     1801 caggtttctg aaggcagagg ataccgaagt gaaggacgga gatgaggttt cgattgtccc
     1861 tgcaatcgcc ggaggaatga ggtagtcagc agtcttgggc agatggtcaa cttcggattg
     1921 tatccttaaa aggaggatgt atgaaaaggc gtgttaagtt aaccttcccg ccgcagctta
     1981 tcagggaacc ggtgatcttt accatggcga aaaggtttga ggtgatgcct aatataagga
     2041 gggcaagggt taccgagacg gttggtgaaa tggtgcttga gttggagggc acagaagaga
     2101 atctcgggaa aggcatccaa tcgctcaggg accagggggt ggatgtggaa cttgtggaag
     2161 gagatatcat agagtagtca tttcacattc ccttggagtt ccgggtaaaa caacatcgat
     2221 tagttaatag ttgagactat aaatgtgaac tttattgtcc gtggatgtcc gtagtgacgg
     2281 cataggacgg tggaagaaat agcatgaaat atatagtgat cgtgggtgat gggatggcag
     2341 ataggccgtt gaaagaactg ggtggcaaga cccctcttca gaaggccttt acgccaaata
     2401 tggacagact tgccaaggag ggcatcatcg gctcggtaag aaccgtgccc agaggccttc
     2461 atcccggctc tgacattgcg aatctgagta tcctgggata cgaccctaag cttttttaca
     2521 cgggacgtgc accgcttgag gctgcgagca tgggtgttga gcttcgagag acagatgtgg
     2581 cataccggtg taatctggtc acgcttcgct ataacaaaga gaggacaaga gcggtaatgg
     2641 aagattatag cggtggtcat gtgtcgactg ctgaggctgc gttactcata agagagatcg
     2701 atgagacact gggaaccagt gatatcaagt tttatcccgg ggtgagctac cgtcatctta
     2761 tggtgtggaa ggacggagaa ctgagggtgg agtgtgtgcc tcctcatgat atccttggaa
     2821 aggatatttg cgattatctt cccacaggct atagagaaga ggttgtcagg aagatgatga
     2881 tggattcggt gggcatcctg agcagccacc ctgttaataa agagcggctt gcagcgggga
     2941 agaagcccgc aaacagtata tggctctggg gacaggggaa gaaacccagg attcccagat
     3001 tcaaagaaaa gtataacctc gatggcgccc ttgtctcggc ggttgacctt accaagggac
     3061 tgggtgtata tgccggattt gaaatcctca gggtcccggg ggtcaccgga tatctagata
     3121 ccaactatac gggaaaggcg gagtattccc tcaatgccct tgagaatgtg gacctcgtat
     3181 atgtccatgt ggaggcgccc gatgaagcag ggcattccgg gaattatgtt gataaggtga
     3241 aggctataga ggattttgat gcccttgtgg tgggtacgat cataaggggt gcaaaacact
     3301 ttcaggcata tcgtatcctg ttgatgcccg atcatgcgac tcccatagag gcaaagaccc
     3361 ataccgatga accgatacct tttgtcattt acgacagtcg tgccataaga aacaatgagg
     3421 gtgccacctt taatgaatcc atcgttggga gaaaagatat catggtggtg gaagaggggt
     3481 ataagctcat ggactatttt atcaaaggaa cgctctaaac gggccctctg ttgagagacg
     3541 tatgcaggaa gggttctcct tcggtgcatt gactcgcgga tcacaaatct tttaaacttc
     3601 agtctaacgt ttttgcttgc ctgccagggt atggtcgtaa cccagaaggg agagttgccg
     3661 gacacaacgt aattcttaaa gagaggtaag aaatgaatga tgagcatata gagaagaaat
     3721 cccctgatgc caagaaggca gaactgcata aggaccgtga agagagtttc gcgtcattgc
     3781 tcaaaaggag cagtatgggt gaaagacttt atccgggaca gaaggtcagg gcgaaggtgg
     3841 taagtatatc gggagacctt gtgtatattg atcttggggg aaagagcgaa ggggccgtgg
     3901 acctggccga atttgtgaat aaagagggat tactcagtat ccgtgaaggt gatgagatag
     3961 aggcgtcttt tgtaacggtt caggacgggt tgatgaaatt gaccaccctt gtgggcggat
     4021 actctgccct cacattgaac tccatcaggg atgcatatga gggagggata tcggtgaacg
     4081 gtgaggttaa acaagagatt aagggaggct ttgaggtgtc ggtaggcgga gtgagatgct
     4141 tctgtccctt ctcccatatc gacctgagag gttcacgcga aggcagtgcc tatgtcggac
     4201 agacgttccc gtttaaggta ttggaatacg gtgaggaggg gggaaaaata gtcctctcca
     4261 gaagggtgct cctcgaagag gaaaagaagg caaggaggga gaagttcaag gagaggctca
     4321 cggtcggcat ggatgtagat gctgaagtaa aatcaataca gaaattcggg gcctttgtgg
     4381 acctgggagg catcgaaggt ttgattcctg cgagcgaaat atcatgggac aggacagtga
     4441 accccaatga tgtactctcc atcggaaaaa acataaccgc aaagatcatc tctcttgact
     4501 gggagcacga caggctcaca ctaagtttaa aggccgcgct gcctgatcca tgggcttcag
     4561 tcgccgataa atacccggtg gatagcaggg tcagcggctc tatagcccga ctcttgccct
     4621 tcggtgcgtt tgtaaggctt gaaccgggga ttgagggcct gatccatatc tccaaccttg
     4681 gagccggcag gcgtatcaat caccccaagg aagttgtaga ggtggggcaa ttggtagagg
     4741 tctatgtcct cgcagtggat cagcagaaca gaaaggtgtc actctccatg cagccaaagg
     4801 ccgagcctgt gaaggtcgtt cttcccgcag cgggagaaat cctcgacggt atcgttgaaa
     4861 aggtgatgcc ttatggaatt tttctgaaaa tgaataatgg cattaaggga ttgattccca
     4921 gtgcggagat ggggacgcca tcaggcacag accacaggcg tatgtttcct cccggaactg
     4981 agatgcaggt tgccgtgatt gatgtggata ccatacataa taaagtccgc ctcagccgta
     5041 aggcggtcct cgaaaaggcg gcacacgacg agttcgccca ttacacggaa tccgcaaaaa
     5101 agtctgctga gtcgtccagg ggcctcggga gtctcggtga catactcaag gcaagactgg
     5161 aagagaaaaa aaaccgtggc tagctcattg gagaaccatt atgtgcagcg ctcaggactg
     5221 acctgacacc tggctt
//
LOCUS       DATKOU010000063        11870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4310470, whole genome shotgun sequence.
ACCESSION   DATKOU010000063 DATKOU010000000
VERSION     DATKOU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4310470"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VMU21_02950"
     CDS             <1..508
                     /locus_tag="VMU21_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HUO76512.1"
                     /translation="APIIKKKIKEVNGKIAGIYYAPDDELVIEARLRELMDSGADLLI
                     TTGGMSVDPDDVTRFAIKRLGARDIIYGSAVLPGAMFLVAYIEKGMQSSKLQIPGKQI
                     RALQQPIPVLGIPACGIYHKTTIFDLILPRILAGEVIGRRELAELGHGGLCLNCHDCR
                     YPVCPFGK"
     gene            561..782
                     /locus_tag="VMU21_02955"
     CDS             561..782
                     /locus_tag="VMU21_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HUO76513.1"
                     /translation="MLREKVEEVLEKIRGGLKAEGGDIELVDIKDNIVYVQLKGACGT
                     CAMSTLTMKNWVEANLKKEIPEIIAVRSV"
     gene            779..2101
                     /locus_tag="VMU21_02960"
     CDS             779..2101
                     /locus_tag="VMU21_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUO76514.1"
                     /translation="MNKEYGAKEVNTLCPVQHEVKPKTRSFSFSLFSVLFFVIALSNS
                     LGAEERAQVKDIRYWSSDGYTRVIVDLSRPVDFAQRRLSSPDRIYFDLRNTRIPKEIQ
                     NSLSVGDGILRSVRAGQYDQATVRIVLDLETMDDFSAYILDDPTKLVIDVNAKKPEKP
                     LRPDRHEKITITRKVLVLDPGHGGHDTGAVGKKGLQEKDVVLDIALKVKEILLREENI
                     EIVLTRDKDVFIPLPERSLIALQHKADLFVSIHANASPNRGAKGIETYLQNWTNEEEA
                     IRVAARENYISEKRMKEKMAQYKNDDVGKILTDLNRDYKRDESIALAHYVQNSLYSNV
                     TKVHAKTVNLGVKQAMFFVLMGASMPPSILAEVSFISNPTEEKLLADNSYRRLIAESI
                     AAGVLIYFESSGPVQKTTKAKHNVAALAKKVSSSDTHSARSQDFLAGR"
     gene            2109..2741
                     /locus_tag="VMU21_02965"
     CDS             2109..2741
                     /locus_tag="VMU21_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HUO76515.1"
                     /translation="MLPYLIIFLYVLFVISLFFIRDIVLHGIIALTVFCIALIVLPLK
                     RVKGGIVPITLFLLFTFAGNLFFQPGRILYGNDLLSVTDEGLLMAGIRTLRVLSMIYA
                     AKILTGILSMDDMLHSLETFLKPLERIGLPVKDFFCVMGLTLKAFPLLMNHLLKTYRE
                     EIKDHEKHGFRRRMRHMVSFLLPVFVESIRSPETFFGSSEHRTLPGEREL"
     gene            2738..4333
                     /locus_tag="VMU21_02970"
     CDS             2738..4333
                     /locus_tag="VMU21_02970"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HUO76516.1"
                     /translation="MNSDVDLLIRGSNVYDGSGDDGFVADVGISGDRIACILKAPERY
                     QGGAGKIIEAKGLSLAPGFIDTHAHSEFTLIADHRAEGKVFQGVTTEINGNCGLSAAP
                     LFGKAREQREDDFQEMGIRERWATFGDYFRLLEKIHPSINYATLVGHGNIRASVIGYD
                     DREPDKGEMQEMRLLLRKGISEGAIGLSTGLIYPPGVCAKTDEITGLAEDINQYIYTS
                     HMRSEGDALIESIEETLTIGKESGIKVHISHIKTGGQANWGKVDEAISVMEHAQREGI
                     HVTCDRYPYTSSSTDLDTVLPSWAYAGGAEEELRRLKDEKVRYSIRGELLSQHPEDDY
                     WDCIAISSLQLESNKWMEGKTLHTLSKAVGKGPVDFLLDILVEERLRIGAIFHSMNED
                     NLARFLSLPYAMIGSDSSARSTDGPTRKGKPHPRGFGTFPRFLGRYARDQRQMSIGKA
                     IHKMTMLPAETFGLKGRGCIRPGYMADLVIFDDQKIKDRATFEEPFLKPEGIHYVLVN
                     GSPVVWEGETTGATTGRILRHGG"
     gene            4323..5072
                     /locus_tag="VMU21_02975"
     CDS             4323..5072
                     /locus_tag="VMU21_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HUO76517.1"
                     /translation="MGDKRVAGKPLIGITLDRDGEYLRLKHQYPPAILRAGGIPILIP
                     HCNDPVLIAETIDGLLIPGGSDIDPSYYSEEAAFMKGEGSISGKKEGCKKDVDVVMSE
                     VQLHTVPRERTDFEMSLLRAIMKLRKPIFGICYGMQLINVAFGGSLYQDIACQFNTTI
                     DHKQGSHRILGKGGTLKGEFTVNSSHHQAVKGLGDGLSVSAFSEDGIVEAITLPDYPF
                     LTGVQWHPERLEDELSLILFSAFVESAHAGK"
     gene            5059..6102
                     /locus_tag="VMU21_02980"
     CDS             5059..6102
                     /locus_tag="VMU21_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUO76518.1"
                     /translation="MPANRFYLFMLLSILTVLGYLSYQIFLPFLTPITWAIVFCVVFY
                     PVYAFSLRFIRLKAMASLLTLMLILVVIIGPFSYISYTLMSEVTDFVGRAEITTKKLA
                     ELLAHEHITKIMEKIQPYTGIEGPSEEIIIENTKKLGKKVVEGLSFGFTNAVSVAGNF
                     VVMAFTIFFLFKDGPGFLEKIRDYLPFSENHRNRLVSQIRDMIVSTIYGGVIVAIVQG
                     ILGGLAFAVLGIGSPVFWGCSMALMSFIPVLGTSVIWFPASLILLFHGAFAKGIALIL
                     IGVLVISMVDNILKPLIIGGRTKMPTIIIFFTVLGGIKVFGLLGLIMGPLVFALFLSV
                     FQIFKAIEGGIDA"
     gene            6597..8066
                     /locus_tag="VMU21_02985"
     CDS             6597..8066
                     /locus_tag="VMU21_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76519.1"
                     /translation="MYYRLGTNPTTLDPALIVDVTGSTIAAKLFNGLVRLDENLNIIP
                     DIAESWSISRDGLSYIFKLSKNVKYNNKKIVTSYDFKYSFKRILDPKTRSPNTWVLEK
                     IEGAKDYLRGKADDVKGLRVIDDHTLEIRLEKPFSPFLYLLTMSAAYVVPEDEVKRWG
                     PDFSSHPVGTGPFMLKEWLTNRELRLERRESYFDKKPHIKGIVYRIIPEDLTAMTEFE
                     LGNLDIVAVPGSEFARYRNSEKWKDLMASVKGINTYYIGMNCARPPFSNVNIRRAMSI
                     AIDRRKLLDTFYEGRGRVAAGPVPDLLRKWQAPDLYEYNPQKARDIVRQEGFAGTTIH
                     FYITAEQEVVDMAEFIQSYIGKTGLKVKIHQLEWSAYKEAINRGEPDMFWLSWWADYP
                     DPEDFLFPLFHSSNMGPAGNRTRYKNQAVDRFIELGQAAGNERERNSYYEKAEQLIVR
                     DAPWVFFWHRNDYTLRQTWIKGYRIYSIYSMDKGTEIRF"
     gene            8207..9472
                     /locus_tag="VMU21_02990"
     CDS             8207..9472
                     /locus_tag="VMU21_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO76520.1"
                     /translation="MKDRDRDKTKEELIRESSALKRQVARLKALIVRHEKTETELQRV
                     TRALKVLSNCNQALMRARDIPSFMKEVCRIIVEVGGYRLAWVGFAEKDEGRTVRPVAQ
                     HGYEEGYLDTVNITWSNTERGRGPTGMSIRTRKPVIARNVLTDRSFAPWRSEAAKRGY
                     ASCIALPLIGGKKVFGSLTAYAAEPDAFDKEEATLLTELANDIAYGIGVMETRSKHRK
                     AEDALKQSFGTLRKVLDGTVYALAEMSQQRDPYTAGHQRRVAQLACAIAREMGLSENQ
                     IEGIRMSALLHDIGKMNVPSEILTRPGQISDLETGIIEAHARAGYEILRTIEFPWPVA
                     STVLQHQERLDGSGYPAGLSDGEILLEARILAVADVVEAMSSHRPYRATLGMSKALKE
                     ISRNRGVLYDPDVVDACLILLTKKKFKFE"
     gene            9491..9754
                     /locus_tag="VMU21_02995"
                     /pseudo
     CDS             9491..9754
                     /locus_tag="VMU21_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
     gene            complement(10291..11034)
                     /locus_tag="VMU21_03000"
     CDS             complement(10291..11034)
                     /locus_tag="VMU21_03000"
                     /inference="COORDINATES: protein motif:HMM:NF006072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUO76521.1"
                     /translation="MDITGKNIIITGGARGLGRKYALDLKALGAKPYVLALHQENLDA
                     LKHEADIPGKVVDVSNEKEVEAFVDNYTQEYGPPDVLINNAGITADCLFIKKRGEEIT
                     KFPYENWQRVININLTGVFLFSREVAYHMVKHGVKGVIINISSISRCGNVGQVNYSAS
                     KAAVDAMTVTLAKELSKFGIRVAAVAPGFIDTEMLQTIREDMLNRIIQTIPLGRLGAI
                     EEISQAVQFIIRNDFFTGRFLEVDGGMRI"
     gene            complement(11125..11562)
                     /locus_tag="VMU21_03005"
     CDS             complement(11125..11562)
                     /locus_tag="VMU21_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO76522.1"
                     /translation="MISSQLMHRDIVTILAMESIQTAAKKMKDKHADVVVVLGEDGIF
                     KGLLTEKEVTMAATNSGFAEQVADYSPKREVLLDLACDDGNICNYFSEVAKEVIMAAT
                     NSGFAEQVADYSPKREALIDFACDDGNRCNYFSEVAVDLLMSL"
BASE COUNT         3114 a         2669 c         3037 g         3050 t
ORIGIN      
        1 tgcccccatc atcaagaaga aaataaagga agtgaacggc aagatagcgg ggatatacta
       61 cgcccctgat gatgaactgg ttatcgaagc aagattgagg gagttgatgg attcaggcgc
      121 tgacctgctg atcacgacag ggggcatgtc tgttgaccct gatgatgtga cacgctttgc
      181 cattaagaga cttggagcaa gggatatcat ctacggttct gcggtacttc ctggtgcaat
      241 gttccttgtc gcatatattg aaaagggaat gcagagttct aaattgcaga ttcctggaaa
      301 acaaatccgc gccctccagc aacctatccc tgtcttagga attcccgcct gcgggattta
      361 tcacaagact actatatttg atctgatcct tcccaggatt cttgctgggg aagtcattgg
      421 cagaagggaa cttgcagaac tcggtcatgg cgggctctgc cttaactgcc atgactgcag
      481 gtatcctgta tgtccctttg ggaaataaaa aaaggacttg aatattagaa aaagtttcat
      541 tattattaaa ggagaacaat atgttaaggg aaaaagttga agaggttctt gagaagataa
      601 ggggtggtct aaaggctgag ggtggcgata tagagcttgt ggatatcaag gacaatatcg
      661 tctatgtgca actcaagggt gcctgtggca catgtgccat gtccactctt accatgaaaa
      721 attgggtgga ggcgaatcta aagaaggaga ttcctgagat cattgctgtt cgttccgtat
      781 gaataaagaa tatggcgcaa aggaagtgaa cacactgtgc cccgtgcaac atgaggtgaa
      841 accgaaaacc cgttcttttt ccttctctct tttctcagtc cttttttttg taatcgcgct
      901 ctccaattcc ctgggcgcag aggagagggc ccaggtcaag gacatcaggt attggtcatc
      961 tgacggatat acaagggtaa tcgtagatct gtcccgccca gtagactttg cgcaaagacg
     1021 gctttccagt cctgacagaa tctactttga tctcaggaat acgaggatcc ccaaggaaat
     1081 acagaattca ctctccgtgg gtgacggcat cctcaggtcg gtgagggcag ggcaatatga
     1141 ccaggccacg gtgaggatag tcctcgatct tgagaccatg gatgacttta gtgcgtacat
     1201 tcttgatgat cccacaaaac tggtcattga tgtcaatgca aagaagcctg aaaagccttt
     1261 gagacctgac aggcacgaaa aaattacgat aacgaggaaa gtgcttgtcc ttgacccggg
     1321 gcatggagga cacgacacgg gtgctgtggg gaaaaaagga cttcaggaaa aggacgtggt
     1381 gctggacatt gcgctcaagg taaaggagat tcttttaagg gaggagaata tagagatagt
     1441 ccttaccagg gataaggatg tcttcatccc gttacccgaa agatctctga tagcactcca
     1501 gcacaaggca gacctttttg tatctatcca tgccaacgcg agccccaaca ggggcgcaaa
     1561 ggggattgag acctatctcc agaattggac taatgaagaa gaggccatac gagttgctgc
     1621 gagggaaaat tacatatctg agaagagaat gaaggagaag atggcccagt ataagaacga
     1681 cgatgtgggc aaaattctca ccgatctcaa tagggactat aaacgggacg aatcaatcgc
     1741 ccttgcccac tatgtacaga actccctgta ttccaatgtc accaaggttc atgcaaaaac
     1801 ggtgaacctg ggagtaaagc aggcgatgtt ttttgtcctt atgggtgctt ccatgccgcc
     1861 ctctatcctc gcagaggtct catttatcag caaccctact gaggagaaac tccttgccga
     1921 taactcgtac cgcagactca ttgcagagtc catcgcagca ggcgtgctta tctactttga
     1981 atcatcaggt cctgtgcaaa aaacgactaa agccaagcat aatgtcgccg cactcgcaaa
     2041 aaaggtttcc tcctccgata cacactccgc caggtcgcag gactttctgg cagggcggtg
     2101 aagagcattt gctgccgtat ctcataatct ttctctatgt actctttgtc ataagcctct
     2161 tttttatccg ggatatagtc ctccatggta tcatcgcgct taccgtattc tgtatcgcac
     2221 tcatagtcct gcctttgaag agggtgaaag gggggatcgt ccccatcacc ctcttcctct
     2281 tattcacttt tgcaggtaac ctcttctttc aacctggcag aatactctat ggcaatgatc
     2341 tgctttccgt gaccgatgaa ggcctgctga tggcaggtat caggacattg agggttttgt
     2401 ccatgatcta tgctgcgaag atattgaccg ggatactttc catggatgac atgcttcatt
     2461 cactggaaac attcctgaaa ccgctggaaa gaatcggtct tccggtgaag gacttcttct
     2521 gtgtaatggg attaacactt aaagcctttc ctctccttat gaatcacctt ttgaaaacct
     2581 atagagaaga gataaaggat cacgagaagc acgggtttcg caggagaatg agacacatgg
     2641 tctctttcct gttgccggtg tttgtggaga gcatccgttc gcccgagact ttttttggct
     2701 cttctgagca ccgcactctt cccggggaga gagaattatg aacagtgatg tggacctgtt
     2761 aatcagggga agcaatgtct acgacgggag cggagatgac gggtttgtgg cagatgtggg
     2821 catatcgggt gacaggattg cgtgtattct gaaggctccc gagcggtatc agggaggagc
     2881 ggggaagatc attgaggcaa agggactttc cctggcacct gggttcatag atacccatgc
     2941 ccattctgaa ttcacgctca ttgcggacca tcgcgcagag ggtaaggttt ttcagggtgt
     3001 caccacagaa attaacggca actgcgggtt gtctgcggcc cctctctttg gcaaagcaag
     3061 ggaacagcgt gaagatgact ttcaggaaat ggggataagg gagcggtggg ctacctttgg
     3121 ggattatttt cgtctcctgg aaaagataca tccttccata aactatgcga cattggtagg
     3181 tcatggaaat ataagggctt ctgtgattgg gtatgatgac agggagcctg acaaaggtga
     3241 gatgcaggag atgcgccttt tgttgaggaa agggatatct gaaggcgcga taggactttc
     3301 caccgggcta atataccctc ccggcgtgtg tgcgaaaaca gatgaaatta ccggattggc
     3361 agaggatatc aaccagtata tctatacttc ccatatgcgc agtgaaggag acgcacttat
     3421 tgagtccatc gaggaaacct tgacaatagg aaaggagtcc ggaatcaagg tgcatatatc
     3481 acatatcaaa acagggggac aggcaaactg gggaaaggta gacgaggcaa tttcggtcat
     3541 ggagcacgca cagcgggagg gtatccatgt gacctgtgac cggtaccctt acacctcttc
     3601 ctccaccgat cttgacacag tgcttccctc ctgggcatat gcaggagggg ctgaggagga
     3661 actgaggagg cttaaggatg agaaggtaag gtactctata aggggtgaac tcctttccca
     3721 gcacccagag gatgactact gggactgcat agccatctcg tctcttcaat tggaatcgaa
     3781 caagtggatg gagggcaaga cattgcatac tctttcaaag gcagtaggta aaggccccgt
     3841 agacttcctc ctggacatcc tcgttgagga gaggttacga ataggggcca ttttccattc
     3901 tatgaacgag gataatcttg cgagattcct ttctctcccg tatgcgatga tcggttcgga
     3961 cagttccgca cgctccactg atggaccgac ccgtaaaggc aaaccccacc caagggggtt
     4021 tgggactttt ccaagattcc tcggcaggta tgccagggac cagagacaga tgagcatagg
     4081 taaggcgatt cacaaaatga ccatgctccc ggccgagacc tttggtctca aaggaagagg
     4141 ctgcataagg ccaggatata tggctgatct ggtgatattc gatgatcaaa aaatcaagga
     4201 cagggccacc tttgaagagc cgtttctaaa acccgaggga atacattatg tccttgtgaa
     4261 cggctcaccc gttgtatggg agggcgagac aacgggtgca acaacaggga ggatattacg
     4321 gcatggggga taaaagggtt gcaggcaaac ccctcatcgg cattaccctt gacagggatg
     4381 gggaatacct gcgactgaaa caccagtatc cgcctgccat actccgtgcc ggagggatac
     4441 ccattttgat accccactgc aatgaccctg tcttaattgc tgagaccata gatggtctcc
     4501 tcattcccgg agggagtgat atagaccctt cctattattc cgaggaggca gccttcatga
     4561 agggggaagg tagcatttca gggaagaagg agggatgcaa aaaggacgta gatgtggtga
     4621 tgtcggaggt acaacttcat acggtaccaa gggaacgtac ggactttgaa atgtccctgt
     4681 taagggctat catgaagtta aggaagccca tattcggtat ctgttacgga atgcagctga
     4741 ttaatgtggc ctttggcggg agcttgtatc aggatatcgc ttgccagttc aatacgacca
     4801 ttgatcacaa gcaagggagc cacagaattc tgggaaaggg aggaacactg aagggggagt
     4861 ttaccgtcaa ctcttcacac catcaggcgg tgaaaggttt gggagatggg ctttctgtca
     4921 gtgcattctc tgaggatggc atagtggagg ccattacgct acccgattat ccctttctca
     4981 caggtgtcca gtggcatcct gagcggttag aggatgagct gtccctgatt ctcttcagcg
     5041 cttttgtgga aagtgcccat gccggcaaat aggttttatc tttttatgct cctctccata
     5101 cttactgttc tggggtacct ctcttaccag atattcctgc ccttcctgac ccccattacc
     5161 tgggccatcg tcttttgtgt tgttttttat cctgtctatg ccttttccct gagattcatc
     5221 agattgaagg ccatggcctc gctcctgacc ctgatgttga ttctcgtggt aattatcggg
     5281 ccattctcat acatctccta caccctcatg agtgaagtga ctgactttgt gggcagagct
     5341 gagataacga cgaagaagct ggcagagctt ctggcgcatg aacatatcac gaaaattatg
     5401 gagaagattc agccttatac cggaattgaa ggaccttcag aggagatcat catcgagaac
     5461 acaaaaaaat tgggaaaaaa ggttgtagag gggctttcgt tcgggttcac caacgcagta
     5521 agtgtggcag gcaactttgt ggtgatggca tttaccatat tttttctttt taaggatggg
     5581 ccgggcttcc tcgagaagat aagggattat ctgccatttt cagaaaacca caggaacagg
     5641 ctcgtatccc agataaggga tatgattgtc tcaaccatat acggcggggt gattgtggcg
     5701 attgtacagg gtatactggg ggggctcgct tttgcagttt tggggatcgg gtccccggtc
     5761 ttctggggct gcagcatggc gctcatgtcc tttatccctg tactgggcac ttccgttatc
     5821 tggtttcccg cgagccttat tcttttgttt catggcgcgt ttgccaaagg tattgctctc
     5881 atcctcatcg gagtattggt gattagcatg gtagataata tactgaagcc tttgatcatt
     5941 gggggacgaa cgaagatgcc aacaattatt atattcttca cggtccttgg aggaataaaa
     6001 gtctttgggt tgttgggact catcatgggc cctcttgtat ttgccctgtt tctctcggtc
     6061 ttccagatat ttaaggcaat agaaggagga atcgatgctt gaccgtaatg agctgaaaga
     6121 gattgcagaa atgcgtgatg aagatgcgac ctatgtggca taggggcctt ttaaaggttt
     6181 tagcccattc tattcatgag tttctttctt ataggagagg catctgtctc tcggcaaaac
     6241 aatagccctt attgactata ccgtggtcta cgcacctaag aacattctgt tcccattttt
     6301 tgtcaaggtc agctcgtgcg gacggcacat aaccctgagt ctcgccgttc gagccggata
     6361 cctctcctga gtttatgaat gcgggatagg aaaggagcgg agcgactttc cttggtgaat
     6421 gggatgatgg ggatggaagg gtaaaggtaa ggaatttgtt ataattgcca atgcccttat
     6481 tatctttatt ctttattccg ttttgcagaa aattaggtgt tctggcttgc ttggcttttc
     6541 tcatatccct gcttactatt tcttcatgct cccatcataa ccgcctggat ggttatgtgt
     6601 attacagact tggtacgaac cccactaccc tcgacccggc ccttatcgtg gatgtcaccg
     6661 gaagtaccat tgcagccaag ctgttcaacg gacttgtaag gttggatgag aacctcaaca
     6721 taatacccga tatagcagag agctggagca tttcccgtga cggcctctcc tatattttta
     6781 agttaagtaa gaacgttaaa tataataata aaaagatagt tacatcatat gacttcaagt
     6841 attcatttaa gagaatatta gacccgaaaa cccgttctcc caacacatgg gttttggaaa
     6901 agatagaggg tgcaaaggat tatctccgtg gtaaggcgga tgatgtaaaa gggctcaggg
     6961 tcattgatga tcacactctc gaaataagac tggaaaaacc cttttcccct ttcctctacc
     7021 tcctgaccat gtcagcagcg tatgtggttc ccgaggacga ggtgaagagg tgggggcctg
     7081 acttttcgag ccatcctgtg ggcaccgggc ccttcatgct caaagagtgg cttaccaaca
     7141 gggaactgag acttgaaagg cgggagagct atttcgacaa gaagccccac ataaaaggaa
     7201 ttgtctaccg cattattccc gaagacctca ccgcaatgac cgaatttgag ctgggaaacc
     7261 ttgatatcgt tgccgtgcca ggctcggagt tcgcaaggta tagaaactcg gagaagtgga
     7321 aagatctcat ggcctctgtg aagggcatta atacgtatta tataggcatg aactgtgcga
     7381 ggcctccctt cagtaatgtg aacatcagaa gggcaatgag cattgccata gacagaagga
     7441 aattgctcga caccttttac gaaggcaggg ggagagtggc ggcaggacct gtgcctgacc
     7501 tgttgcggaa atggcaggca cccgatcttt acgagtataa ccctcaaaag gcgagggata
     7561 ttgttcgtca ggagggattt gccggtacta ccatacactt ctatatcacc gctgagcagg
     7621 aggtggtgga catggcggag tttattcagt cctatatagg aaagacaggg ctgaaggtaa
     7681 agatacacca gcttgaatgg agcgcctata aggaggcgat taacaggggg gagcctgaca
     7741 tgttttggct cagctggtgg gcggattatc ccgatcccga ggatttcctc tttccccttt
     7801 tccattcgtc caacatggga cctgcgggga accgaacgag atacaaaaac caggcagtgg
     7861 acaggttcat tgagcttggc caagctgcgg ggaatgagag agaaaggaac tcatattacg
     7921 aaaaggccga acaactgatt gtgagggatg cgccatgggt cttcttctgg cacaggaatg
     7981 attacacgct gaggcaaaca tggatcaaag ggtacagaat ttattccatt tacagtatgg
     8041 ataagggaac agagatacgc ttttgagctg tgaaatgagg agagggtgtc ttcaatgatg
     8101 cgtggccatt gtgtcttcat atcacactgt gcggctcact tctgtgctgg acttaaacga
     8161 agaatcctca tacaatggaa tgagaatctt tttccgaagg ttggatatga aagatagaga
     8221 cagggataag acaaaagagg agcttataag agaatcttct gctttgaaac ggcaggttgc
     8281 cagattgaag gcattaatag taaggcatga gaaaacagag accgagttgc agagggttac
     8341 gcgggctctc aaagtcctca gcaactgcaa ccaggcgctc atgcgcgcaa gagatatccc
     8401 gagttttatg aaggaagtat gccgcattat tgtggaggtg ggtggttatc gtctggcatg
     8461 ggtcggattt gcggagaagg acgagggcag aaccgttcgt cctgtagccc aacatggcta
     8521 tgaagaaggg tatctcgaca cagtcaatat cacatggtca aatactgagc gtgggagggg
     8581 gcccacaggg atgtccatac gaacacgaaa acctgttatt gcgaggaacg tcctcactga
     8641 tcgcagcttt gctccttggc gatccgaagc ggcgaagcgc gggtacgcat catgcatcgc
     8701 tctgcccctg attgggggaa aaaaggtgtt tggctccttg actgcgtatg ctgccgagcc
     8761 tgatgcattt gataaggaag aggcaaccct cctgactgag ctggcaaatg atatagccta
     8821 tggaattggt gtgatggaga cacgttccaa acacaggaaa gcagaagatg cactgaaaca
     8881 gagctttgga acgctcagga aggtcctcga tggaacggtg tatgccctgg cagagatgtc
     8941 gcaacagagg gacccttata ccgcaggaca ccagcgacgt gttgctcaac ttgcctgcgc
     9001 cattgcccgt gagatgggtc tctcagaaaa ccaaatcgag gggattcgca tgtcggcact
     9061 tttacatgat atcgggaaga tgaatgtgcc ttccgaaatt ctgaccagac ccggccagat
     9121 aagtgatttg gaaaccggga ttatagaagc ccatgcccgg gcagggtatg aaatactgag
     9181 gactatagaa ttcccatggc ctgttgcgag caccgtactt cagcatcagg aacgtctgga
     9241 tgggtcaggc taccctgcag gcctgtcgga cggggagatt ctccttgaag caagaatcct
     9301 ggctgtggct gatgttgtgg aggccatgtc atcccacaga ccctatcgcg cgaccctcgg
     9361 aatgagtaag gccttgaagg aaatttcccg gaacaggggt gtgctctatg accctgatgt
     9421 ggtggatgcc tgcttgattc ttttgaccaa aaagaaattt aaatttgagt aatcgggaac
     9481 aggagctgat tgcatctcca tcgatgccgg ggtgtccatc ggcgaggtat acgaactcaa
     9541 ccgtgaacag ctcgtgactg cgggaaatgt ggatgtaatt aatacggtat tcggcggtga
     9601 gcccgccctg ctgtgcaaag cagtttcgaa atgcgtgggg gagatttctg atccctatcg
     9661 gaactacatt ctcatgccat cctgcgacct gcctcccgac accccggtgc atgacgtaaa
     9721 ggaatttctc gcctgcgctg accggatcga gtaaatggca attatgaggt acctagccaa
     9781 tgactccata aaagtcttta cgcagtggag cgaaatctta ctgtcatatt gtaggacagg
     9841 gttcgccact ttcactatta cctctgactc aagggacggg gagggatagt gctgtagggt
     9901 tttaatcgaa acttggcacg ctcgcaagct gcggtgtcca ccgactaaac acttttccaa
     9961 aagtgaaaca gttgaggatt tctcctgaaa tccacacaaa gaaagaagga ggcaatagcc
    10021 ccgccctttc tcatatgacc gaactccgcc agcgaaggcg cggacgtaaa tgagggaaaa
    10081 aagcgcggtt ggtataatat gaaaaaattg tcttgctcat ttccgggtat actttacctc
    10141 ttaggcaacg tcttacgtat taacctcaca aatgaatact tgcggttaga taagagttgt
    10201 tatttacgcc agctggacag cactgaccta tatgtggaac attttacaaa ataccagttc
    10261 actcgatagc ataaatttga gctaacattg tcaaattctc atcccaccgt caacttccag
    10321 gaagcgtcca gtgaaaaagt catttcttat gatgaactgg acagcttgtg aaatttcctc
    10381 tatcgcgccg agcctgccca gcggaatggt ctggatgatc ctgtttagca tgtcctctct
    10441 aatcgtttgc aacatctcgg tatcgataaa tccgggggcg acggcagcaa ctcttatccc
    10501 gaactttgaa agttcttttg ccaacgtcac tgtcatcgca tctactgcgg ccttggaggc
    10561 agaatagttc acttgcccca cattgccgca gcggcttatg gaggatatat tgattattac
    10621 accttttaca ccgtgcttca ccatgtggta ggcaacctcc cttgagaaca agaatacgcc
    10681 cgtgagattt atgttaataa ccctctgcca attttcataa ggaaatttcg ttatctcctc
    10741 ccctcttttt ttgataaaga ggcagtctgc cgttattcct gcgttattga taagtacatc
    10801 tggaggaccg tactcctggg tgtaattatc gacaaaggct tccacctctt tctcgttgct
    10861 tacatcaacc accttgcccg ggatatccgc ctcgtgtttc aatgcatcga gattctcctg
    10921 gtggagagcc aaaacataag gcttggcacc gagggccttc aggtctaatg cgtatttccg
    10981 tcccagccct cttgcgccgc ccgtaataat tatgtttttg cccgtgatgt ccatgacgtt
    11041 catgctttca gcaatatgcg ttcagattta cgaactattt ggagagaagg aattgccttt
    11101 ctctcgaacc tgttcttcaa aacactaaag gctcatgagc aggtcaacag caacttcgct
    11161 aaagtagttg catctatttc catcatcaca agcaaagtct atcagggctt cccgcttcgg
    11221 actgtaatct gccacttgct ccgcaaagcc ggaatttgtg gcagccataa ttacttcctt
    11281 agcaacttcg ctgaagtagt tgcatatatt tccatcatca caagcaaggt ctagcaggac
    11341 ttcccgcttc ggactgtaat ctgccacttg ctccgcaaag ccggaatttg tggcagccat
    11401 tgtcacttcc ttttccgtca ataaaccctt aaagatcccg tcttctccaa gaacaacaac
    11461 cacatctgca tgtttgtcct tcattttttt tgcagcagtc tggatcgatt ccatagccag
    11521 aatggtaaca atgtccctgt gcattagctg acttgatatc atgatttgtc ctccttgggc
    11581 caattccccc ttggccaaaa tagacccgat tttcccagct cacctccttt ttttggcttt
    11641 atcgggatta ataattgaga atttacgcta tttgttaatt ctattcttaa ctcacgagtt
    11701 tgtcattgat atttatcaag aaatctgtgc ataaatatca aggtgttaag acttttcaaa
    11761 cggataatac tcagacttgt gataggttct tcagtattga ggaggcaaaa gaggctgaac
    11821 aggatttaca acccacatcc ctgcttgagg ctttgggtat cgttgacgat
//
LOCUS       DATKOU010000064        18674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1612375, whole genome shotgun sequence.
ACCESSION   DATKOU010000064 DATKOU010000000
VERSION     DATKOU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 18674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18674
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612375"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..1275
                     /locus_tag="VMU21_03010"
     CDS             562..1275
                     /locus_tag="VMU21_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76523.1"
                     /translation="MVFYPIDQTWSTSTIMAYGVSVPKDMTLQSIEDLVKRRVKEFHL
                     KGSPKYTIQAKDKVDLPNGKTVYVYFFQGADWGNYEAIGYVEEAETINFLVYNARNKV
                     EFEKHLPAFRSIIATYRNVFENATARDDVKFNSLIAEAKALEKTKEGKEYSSEVIQSF
                     GSSLANIIKYCTSFTTKGERAHFDLLFRIRPDGTVSEAYIRPTNALNACVRGLVLDTK
                     HPPHKLDPFLMYVEMNVTE"
     gene            complement(1736..2467)
                     /gene="thyX"
                     /locus_tag="VMU21_03015"
     CDS             complement(1736..2467)
                     /gene="thyX"
                     /locus_tag="VMU21_03015"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HUO76524.1"
                     /translation="MPETRLKVILLRSTPNPEETIAMAAKLCYSPADIDSLKEKIETK
                     DQQNFVERLTKMGHMSPIEHSSFTFAIEGISRACSHQLVRHRLASYSQQSQRYVSEEA
                     GFDYVIPKSIEGDKELERYFKTFMSKAQKAYNHIVTRLNEKGIKGESANQDARFVLPN
                     ACETKIMVTMNARELLHFFKQRCCNRAQWEIRAMAIEMLKLVREIAPAIFSEAGPSCV
                     KGACPEGEYTCGKIAEVRNYFKTIK"
     gene            complement(2565..3518)
                     /locus_tag="VMU21_03020"
     CDS             complement(2565..3518)
                     /locus_tag="VMU21_03020"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO76525.1"
                     /translation="MSNSRIPTVSVIIPTFNRSQTIGRAIQSVLAQTFQDFEIIIVDD
                     ASTDGTKTIVEGLCGPLVRYLRHERNQGAAAARNTGISAAHGIYLAFLDSDDEWLPEK
                     LSIQVTLLDDQSAGLDLSCSSFILVTNESEREYIQTARPDWFKRLLWTCDLGPGSTLV
                     VRRECADRVGFLDEKLSRLEDWDWLLRLSKKHTIAVVEKPLSRIHRGPLPCADVIEAS
                     MARFLENHDEDFGLFGWHYRRRVIAKHWAELSYQFFREKRLLRGGHYLLKAFSVNPLQ
                     NPVLLMWLCSAFVDALLGTSISLWALERKHAAGEALRKLRK"
     gene            complement(3511..4437)
                     /locus_tag="VMU21_03025"
     CDS             complement(3511..4437)
                     /locus_tag="VMU21_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO76526.1"
                     /translation="MKDRPLISIITPSLNSVRFIAEAIESVVSQDYSDIEHIIIDGGS
                     ADGTLELLRTYPHVRVISEPDRGMYYALNKGIQMVQGEIIGQLNSDDFYEKNVFAEIA
                     KLFTEHPTIDAIRGGATVLEDQADGSRRSVAQYVVPPGSGASFRDIVLGVPVINAYFF
                     RSRVFKQYGLYNIFYTISADKDFLIRLALAGFQCSYLPRVVYHYRQHAGSLTINRQTV
                     PWFKIRNEHLHMAERYLQTDQISPEIKSVFELWHKRESAEGALQALREVKPLKAIKYG
                     FLGWRHNPWWPLAFFSYFFSKVAHLAMRRRNE"
     gene            complement(4495..5337)
                     /locus_tag="VMU21_03030"
     CDS             complement(4495..5337)
                     /locus_tag="VMU21_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO76527.1"
                     /translation="MKQVPLVINGIDATEAKTSNEKIPLTAIVVTYNEAPRLRNCLES
                     LAFCDQLLVVDLGSADESVRIAQECGAEVFHHDWVPFVERVLRYALSFARNDWVLRFD
                     PDEAFPKKLVNHLMPSLVQTHVAIVGLPYQYYFLGKPLRTTIWGGKRMMSRKLFHKDR
                     VEISPYVHQALQCKPGYAQINVEPLGDDAIQHFWVDSFEQLSEKHRRYLRYEGESRYR
                     TGQRFAWHKMFYNTLRSLGENLVYRRGLFGGFSGIFLSFYHSWYVCRGWLSLREYERN
                     LSHR"
     gene            5471..6847
                     /gene="argH"
                     /locus_tag="VMU21_03035"
     CDS             5471..6847
                     /gene="argH"
                     /locus_tag="VMU21_03035"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HUO76528.1"
                     /translation="MKKLWGGRFREKTSSIVEAFTESISFDIRLWKHDIEGSIAHARM
                     LGKQGIISKNDSEKIVRGLKEIAKEIETGKFRFREDLEDVHMNIEAALTKKIGPAGKR
                     LHSARSRNDQVALDLRLYLREEIGLILSAVTRFQTSLVNIAETHIDYIMPGYTHMQRA
                     QPVLLSHHLLAYVEMFERDKGRLRDALKRVNVLPLGACALAGTSLPIDRGYVAKLLGF
                     DSVAENSIDAVSDRDFGMEFLSASAMFMVHLSRMAEELVLWSTEEFSFAQLPDAFTTG
                     SSIMPQKKNPDVAELIRGKTGRVFGGLFSLFTMMKGLSLSYNRDMQEDKLPLFDAVDT
                     IKACLAVLTEMVPRIRFDKKRMSGTAAHAYSTATDMAEYLVRKGMSFRDAHGVTGRVV
                     LYCIEKKKALHELKVGELQRFSPLIQGDIYRYLTVEGSVGSKRSRGGTAATEVKKQIT
                     RLRGLLTQ"
     gene            6844..7770
                     /locus_tag="VMU21_03040"
     CDS             6844..7770
                     /locus_tag="VMU21_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76529.1"
                     /translation="MIFRVLLPFLFFISLVIPACGRKAPPTLKAYEKPQAPAGLTVIH
                     REGKILVSWSYPGNLQSSIKGFQVMRAEGEGFERRGFVGNEGNSFPDDSFSPDTTYKY
                     KVMAQNLKDILSDDSTIVTITPKLLPPPPEDVQVSVGNDAVQLLWQSSGEGVCYHVYR
                     TTEKGRYGDVPIHRDPICTTSFSDRTLFQDKPVYYTIRALHNTPLLDEGYPSAEVEVT
                     PSHFIPSAPAELRGVRAQGKIVLMWRESPETWVKGYRVYRKKEREKEFTFLGEVKTPT
                     FSDTGKVGKKVRYRVTALGPSQESEPSVTEVP"
     gene            8080..8673
                     /locus_tag="VMU21_03045"
     CDS             8080..8673
                     /locus_tag="VMU21_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HUO76530.1"
                     /translation="MPWMEEKIVKAFEESISVKQNFFKENVGAIVEASKMIASAFTEG
                     KKLLLFGNGGSATDASHMAAEFVNRFKRERPGLPAIALTTDMAVITSIANDYDYSEIF
                     ARQIKSLSDEGDLVLAISTSGSSGNVLKAMDVAKRKKLKTIVFTGAKGEKFASKADCA
                     FIVPSDNTPRVQETHITLGHVLCQMVEEILFESPRKR"
     gene            8722..9207
                     /gene="ruvC"
                     /locus_tag="VMU21_03050"
     CDS             8722..9207
                     /gene="ruvC"
                     /locus_tag="VMU21_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HUO76531.1"
                     /translation="MIILGIDPGLASTGFGAIQCGKATSSLLTCGYIKTSPRQHISHR
                     LFQIHSDVNHLIRSLHPDLIAIENVFSLVKYPKAGILLGGVLGIIYLSVFQNDVSMME
                     ITPREVKNSLAGYGGADKDQMKRTVKKLLNIPDLKSFHASDALAVALTAFYRRTSWGG
                     R"
     gene            9192..9821
                     /gene="ruvA"
                     /locus_tag="VMU21_03055"
     CDS             9192..9821
                     /gene="ruvA"
                     /locus_tag="VMU21_03055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HUO76532.1"
                     /translation="MGGPLISYLKGKVRLNRSDDKITLIVNGIGYDILIPAYVMSGIK
                     GSRADGDEMELYVSYNQTERQPKPVLVGFESPLDKEFFELFISVEDIGPTAAIRALTK
                     PVREIARSIEGKDTKALKALKGIGERKAEKIIATLKGKVAKFALMPEVEVPVGVAEDF
                     RKEVEDVLMNQLGHKPMEARKMIEEAMKRNPRICSSEELFEEVYRGQGQ"
     gene            9818..10873
                     /gene="ruvB"
                     /locus_tag="VMU21_03060"
     CDS             9818..10873
                     /gene="ruvB"
                     /locus_tag="VMU21_03060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HUO76533.1"
                     /translation="MIHEEGDPLSEILKENSSLDEDAITLRPRRLTEYIGQDKIVETL
                     KIAIEAALQRKEPLDHILFNGPPGLGKTTLAHIIANEMGSKIITSSGPALEKGGDLMG
                     ILTHMEKGDVFFIDEIHRIPKIVEEFLYPAMEDFAVDFIFDKGVHARTHRYRLEPFTL
                     IGATTRAGLLSSPLRERFGITRDLDFYQDKDLVQIVKRSSAILEVTIEQDGSYEIARR
                     ARGTPRVANRLLKRVRDYAQVRAQGKITGQVAKEALALEGVDEEGLGETDRKLLKTII
                     LNYKGGPVGIEALASTLQIETDVLLDVVEPYLLKSGFIIRTSHGRKATEKAYHLLNIP
                     HKGEIGLFEGAKGKKHP"
     gene            10883..11722
                     /locus_tag="VMU21_03065"
     CDS             10883..11722
                     /locus_tag="VMU21_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUO76534.1"
                     /translation="MGISDHFIPLPTWPAQKAREFFQEKNEEAYNLVDVRHPREYEVG
                     HLPGAQLIPLDELPDHLGDLDPEKLTITYCAKGIRSRAAAAFLSGSGFKEVYSIEGGL
                     EAWHGLVAEGPPDSGMAYFSPAAGPGELIALAWLLEDGSRKFYYALSELLDNKDATVL
                     FRELTLAEEHHMSSLLTLYTQFSGKEPDAGFPWSVLSLEPEGDIMEGGIRVDEALQWV
                     QGKEVKDILELAIANETNSYDLYLRMERTIEDTRSKKIFGTLSEEEKAHLQKLIALSE
                     NSL"
     gene            complement(11907..13610)
                     /locus_tag="VMU21_03070"
     CDS             complement(11907..13610)
                     /locus_tag="VMU21_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HUO76535.1"
                     /translation="MHNLFRPRPDLILLHPPTVFRFRERPGFYGPMSDVIPSSSIFEI
                     YPIGFLAISDYLYRHGIPVRIVNLAMKMLRAESFNPEKFIKKLDALAFGIDLHWLPHA
                     DGSLCLAEMLKKHHPGKPVILGGLSATYYHGEIMADYPFVDFIVLGDSAEEPLRMLME
                     AIKSGTGFESVPNLVWRDGSGKVITNELSNRPENLDDINFDYLHLLKMAIKYRDPSGY
                     IPFRYWLSYPVTAVFQCRGCVHNCASCAGSLSSFKRVCMREKPCFRSPELLAEDIRKI
                     SEYTGAPVMVIGDLLQAREDYAGRFLDAVKKYRIKNELTVEFFTPPPETFIRKVADCI
                     ENFNVEVSPESHDPKIRKAFGKHYENKELEQMIATLLDAGCKRIDIFFMVGLPYQDYR
                     SVMETVGYCEELLRKFGRTRRLLPMIAPLAPFVDPGSGIFEEPEKFGYRLLYKSLKEH
                     RQAMLMPSWKYTLNYETEWMTREDIVKATYDGALMLLDIKEKYQVIGKEKASMIREHI
                     EKAKVLIEHIDSSGQIDDATRTEILHLNNLASLCDKRELDWPIKGWRLNLPKIFKSLL
                     K"
     gene            complement(13610..14545)
                     /locus_tag="VMU21_03075"
     CDS             complement(13610..14545)
                     /locus_tag="VMU21_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HUO76536.1"
                     /translation="MEKELICRPMIMFGGKGGVGKTTCSATAALQLASSGQKTLIITS
                     DATPSLSDIFEQEIGDTIRSIEERLDAVEISDDAILNKWKKKFGPDFYEILSHLIDVE
                     GLDNESRHQLLDYIGSAPSLREETMLDLIVDLAETGTYDRIVWDTAPAGETLNLLNMP
                     TYIRNHLSSGSRVFEGLDKIGKKIAGKRSIARIMDEWIAASERISRYILRSAAFVVVA
                     NPEALVVKQARRVVETLRGYHITLQGMIINRVINDPDSPSFRTLQETQELYLADLIRL
                     ADGLPVAMLPFSLKEIKGKNALREIGVGLIKELKL"
     gene            complement(14656..14856)
                     /locus_tag="VMU21_03080"
     CDS             complement(14656..14856)
                     /locus_tag="VMU21_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76537.1"
                     /translation="MGFFALLGFIIWALLMVLIFRKAGYSGLQIILLFVPVVNVILFI
                     WFALTEWPIQKELERLRSGRGM"
     gene            complement(14929..15246)
                     /locus_tag="VMU21_03085"
     CDS             complement(14929..15246)
                     /locus_tag="VMU21_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76538.1"
                     /translation="MGGVITSAVTYLANNSKGLLAAFIGTLPIITISTFLLIYFTAGQ
                     TAVLAYAKGLIIMIIPWMIFILTVIFLSPRLNFISSLITGIILQIAIALLIMNFDKIY
                     FRS"
     gene            15446..17869
                     /locus_tag="VMU21_03090"
     CDS             15446..17869
                     /locus_tag="VMU21_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76539.1"
                     /translation="MKTIDQHRVGRLLMRWSLSAAVAMAVLASYSPARADWNWPREVQ
                     VKDGTLTIYTPQIEAFRGDKVDGRAAIEMVPKDGKETTFGVFWFSARLATDRDSRTVS
                     LEDIKVTGVKFPEKAGAQDTQAAEAILRQGGGLSSMSMSMDTFVAITAVLPREKVAPE
                     KFNNAPPKIIVVKVPTALVIINGKPIMRDVPGTAVNRVVNTPFVMLYDKSAKAYYLKG
                     GKWWYQATDIKGDWKTTDTPPEAIVQAALKVSEPEPPELKAFTQEEGSAAPAVIVATE
                     PTELIVTDGDPQYAVIPGTDLLYVSNTGNDIFMDVNSKQFYVVLAGRWYRSPSLAGGK
                     WNYVLSQKLPAAFYRIPPGSDKGYVLAFIADTKQADEAVMEAQIPQTSAIKRSEATAD
                     VAYDGSPEFKPIKDTGLEYAVNTGSQVIKVNGKYYLAQKAVWYVSDSPTGPWVVSDHT
                     PPNIESIPPDSPVYNVKYVRVYDATPDVVHAGYTSGYVGSYVDNGTVVYGTGYDYPGW
                     EGTGYYPAPLTWGLSPWYYPYYGEWGFGAGFVSGAFLGFVTGAIVSPWWGWGGWWGPW
                     WGGWWGPWWGGWHGGPWSGGHWGGYWGWGWHAQQWRHGNYKGTWQHGWPSSNLYNRPG
                     STAWNTQAHRGPQAGPGGRASLMPTNMARASAQPTGSGTRYRNVMPGGTAGTRPGGGA
                     AVRQNRPSGTMQGAQRYGAARQNNVFASPNGGVFRRTQQGWQQYNRGGWAQPQSGYSR
                     NQSLLNRDYNARMRGIQRESSFGGYSRGGHSSGVSRGGGFSSSGRSGGMSGGGSHGGG
                     VGHGGGGKR"
     gene            complement(18106..18453)
                     /locus_tag="VMU21_03095"
     CDS             complement(18106..18453)
                     /locus_tag="VMU21_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76540.1"
                     /translation="MELKYFLYFFFGGLITSAVTYLANNSRGLLAAFIGTLPIITIST
                     FLLIYFNAGQTAVLAYAKGLIIMIIPWMIFILTVIFLSPKLNFISSLIIGIIIQIVIA
                     LLILNVDKIYFRP"
BASE COUNT         4842 a         4520 c         4898 g         4414 t
ORIGIN      
        1 aatttagacg tgtgaagggc tacagggaaa tcccaaaact cattgctgcc ttacagcgaa
       61 aatccattga cagaaaggag gttgcagctt aggatatgaa ccggggtcct ttctacttca
      121 acgaaaggac gggacaactt cttagttata ctctttacac ctataaggtg aaatgctcta
      181 cagactcatt caggtcaata aaagctacaa catataattc gtcggagagt gcaatcaagg
      241 ggacagatct cgattatatc gaaacgggac aagcgacgtg ggagcatata acacctaact
      301 cgatgatggc tcttttatct gaagaatgct gtaagtatct gctatacgac ggtgggtaag
      361 gccagcgttg ggtgcaaagg aaaaatcatg agactgatac cgttgattct gatgctcgca
      421 aattttcttc cgagccctag tcttgcggct attacggagg gcggaacagg catgctgttt
      481 ggggccgatc acgctttcac tttcactgca ccgaaagggt ggggtttgga caatcaaagc
      541 ggtgttaagc aaggtctgca tatggttttt tatcctatcg accaaacctg gtcaacgagt
      601 accatcatgg catacggagt gtcggtacca aaagacatga cattgcaatc gatcgaagat
      661 ctggtgaaaa ggagagttaa ggaattccat ctaaagggca gccccaagta tacgatccag
      721 gcgaaagaca aggtggattt gcccaatggg aagacagtct acgtctattt ctttcagggt
      781 gccgactggg gtaattacga agcgattggg tacgtggaag aagcggaaac catcaacttc
      841 ctggtgtaca acgcccgaaa caaggtggag ttcgaaaagc atcttcctgc ctttcgatcc
      901 ataatagcca cttacaggaa cgtgttcgag aatgccacgg cgagggacga cgtgaagttc
      961 aactcactta ttgctgaggc aaaagcgtta gagaagacga aggaaggaaa agaatacagt
     1021 tcggaggtca ttcagtcatt tggcagttcc ctcgcgaaca taataaaata ctgtacatcc
     1081 ttcaccacca agggcgaacg ggcccatttt gacttgttat tcaggataag acctgatggt
     1141 acagtatctg aggcatacat cagaccaacc aatgcgctga atgcttgtgt tcgtggtctc
     1201 gtgctcgaca ctaagcatcc tccccacaag ttggacccat tcttgatgta tgtcgaaatg
     1261 aatgtcacag aatgataaac cgaaagaaca tcatccaccc aggggatgca cccaatcttt
     1321 ccccgcagac ttcttccgta aatcctcact gctacaataa atcgaaatgt gtggactagt
     1381 cggtggagaa gagattgcag tttgcctgaa atttatgtca aggatcgcgc atagttcagg
     1441 gcaaagctga cgtcctctgt ttataacttc gccatccatt aaattccaaa agtaacaagc
     1501 accattcgcc aggtctccga ccactgaggc ccacgaagag acgcctttcg accggcgata
     1561 atatgacgcg tgttttggga cgaagtcttt ttcagttgcg gatcgacata cctaacaagt
     1621 atcaggtgta ttcaacattt cggaagatgt gtctggtccg tcaactccag cgaaaagccg
     1681 cgaatcggat ggcatgcgta cggcaggaac taatggtatt caccaaatta tatgatcact
     1741 taatcgtctt gaaatagttc cgaacttccg ctattttccc gcaggtatat tctccttccg
     1801 gacacgcccc ttttacacat gaaggtccgg cttctgagaa gattgccggg gctatttccc
     1861 tcaccaactt aagcatctca attgccatcg ccctgatttc ccactgagcg cgattacaac
     1921 agcgctgttt gaagaaatgc agtaattcgc gggcattcat tgtaaccatg atctttgtct
     1981 cgcacgcatt gggaagtaca aagcgggcat cctggttggc agattcaccc ttgataccct
     2041 tttcattaag cctggtgaca atatgattat aggccttttg ggcttttgac atgaaagtct
     2101 taaagtaacg ctcgagctct ttatcacctt ctatgctctt gggtatcaca tagtcaaatc
     2161 ctgcttcttc gctgacataa cgctgcgact gttgtgaata gcttgccaac ctgtgtcgta
     2221 ctaactgatg agaacacgcc ctcgaaatgc cttcgattgc gaaggtgaaa gaagaatgct
     2281 caatgggact catgtgaccc attttcgtca gcctctcaac aaagttttgt tgatctttag
     2341 tttcaatttt ctcttttaac gagtcaatgt ccgctgggct gtaacagagc ttcgcagcca
     2401 tggcaatggt ttcttcgggg ttgggagtag accgcaaaag tattactttt agtcttgttt
     2461 ccggcattta gctatcatat catatttcaa aaacccttga cagagtaacc caaaactttt
     2521 attcttagcc atgaagaaac tctggggtgg aaggttatgt ggcttcactt gcggagcttt
     2581 ctcaatgctt ctccggcagc gtgtttacgc tcaagggccc atagtgagat tgatgtccct
     2641 aagagggcgt ccacaaatgc actacataac cacatcaaga gaaccggatt ttgcaatgga
     2701 ttgacactaa aggcttttag aagatagtgg cctccgcgaa gaagccgttt ctcgcgaaaa
     2761 aactgatatg acagctcggc ccaatgtttg gcgatgaccc ggcgacgata atgccatccg
     2821 aatagcccga aatcctcatc atggttttcc agaaaacggg ccattgatgc ttcaatcaca
     2881 tctgcacagg gcagcgggcc tcggtggatt cgtgacaagg gcttctcaac aacggcgata
     2941 gtatgtttct tggacaagcg aagcaaccaa tcccaatcct ctaagcgaga taacttttca
     3001 tccaagaatc cgactctgtc tgcacactct ctcctgacta caagcgttga acctggaccg
     3061 agatcacatg tccagaggag gcgtttgaac caatccggcc gggcagtttg tatatattcc
     3121 cgctcactct cattggttac caaaataaaa cttgagcagc ttaggtccag gccagcggat
     3181 tgatcatcga ggagcgtaac ctgtatgctc agcttctctg gtaaccactc atcatccgaa
     3241 tccaggaagg ccagatatat tccgtgagct gcactgatcc cggtgttccg agctgctgct
     3301 gccccttgat ttcgttcatg gcgcagatag cgaactagag gcccgcaaag tccttcgacg
     3361 atggtcttcg ttccgtcggt ggaggcgtcg tcgacaataa tgatctcaaa atcctgaaat
     3421 gtctgcgcta aaacgctctg gattgcccga ccgatggtct gactccgatt aaaggtcggg
     3481 atgatcacgc tgactgttgg aattctggaa ttactcatta cgtctcctca tggccaaatg
     3541 ggcaacctta gagaaaaaat aggagaaaaa ggctaaaggc caccagggat tatgacgcca
     3601 accaagaaag ccatatttta tggctttcag aggcttcact tccctcagtg cctgaagcgc
     3661 gccttctgca gattccctct tgtgccaaag ctcaaagacg ctctttattt cgggagatat
     3721 ctggtcagtc tgcaaatagc gctctgccat atgcagatgc tcatttcgaa tcttgaacca
     3781 cggaacagtc tgcctattta tggtgaggga gccagcatgc tgacgatagt ggtacaccac
     3841 acgtggtaag tacgagcact ggaacccggc gagggccagc ctgatgagaa aatccttgtc
     3901 cgcggaaata gtgtagaaga tattgtaaag accatattgt ttaaagacac ggctacgaaa
     3961 gaaatacgca ttgatgacag gaactccgag tacgatatcg cggaaagacg ccccggagcc
     4021 ggggggaaca acgtattgag ccacggagcg acgcgaccca tcggcttggt cctcgaggac
     4081 cgtcgcgcca ccacggattg cgtcaatagt agggtgctct gtaaataatt tggcgatctc
     4141 agcaaaaacg tttttttcat agaagtcgtc gctgttaagc tggccgatga tttctccctg
     4201 aaccatttga atacctttgt tcagtgcata gtacatgccc cggtcgggtt cgctgatgac
     4261 gcgcacatga ggatacgtgc gcaaaagttc gagtgttcca tccgcagagc caccatcgat
     4321 gataatatgc tcaatgtcgg agtagtcttg agagacaacg ctctcgatcg cctcggctat
     4381 gaagcgtacg ctgttcaggc tgggcgtgat gatcgagata aggggtctgt ccttcaattt
     4441 aagcccttcg gtcaattacc gaaagaatga cttggggaac ccctcgggct ttccctatct
     4501 atgtgaaagg ttacgctcat attcccttaa cgaaagccag cccctgcaca cataccaact
     4561 gtgataaaag ctcaaaaaga tgccagagaa gccgccaaac aatccccgac ggtatactaa
     4621 attctctcct aacgacctca gtgtattata aaacatttta tgccatgcaa atcgctgacc
     4681 ggtcctgtat cttgactccc cttcgtaccg gagatagcga cgatgttttt cagataactg
     4741 ttcgaaactg tccacccaaa agtgctgtat tgcatcatca cccaggggtt caacattaat
     4801 ctgtgcatag ccaggtttgc actgaagcgc ctggtgcaca taggggctga tctccaccct
     4861 gtctttgtga aataacttcc tgctcatcat tcgtttccct ccccatatgg tggtgcgtaa
     4921 cggttttcca agaaaatagt actgataagg taatcccacg atcgcaacgt gcgtttgaac
     4981 aagtgaaggc atcaggtgat tgactaattt ctttgggaaa gcctcatcgg gatcgaaccg
     5041 aaggacccag tcatttcggg caaacgacaa ggcataacgt agcactcgtt caacaaatgg
     5101 tacccagtcg tggtgaaaga cctctgcccc gcattcctgt gcaatacgca cggactcatc
     5161 tgcagagcca agatcgacaa caaggagctg atcgcaaaat gcaagactct ctaagcagtt
     5221 tcgcagacgg ggcgcttcat tataggtgac cacgatggca gtgagcggaa tcttttcgtt
     5281 ggatgtcttt gcttccgtag catctatccc atttatcacg agaggaactt gtttcattac
     5341 ggataagtca tagtatacat ccggtaaaag gaatattcat agtccatcgc ctcacagcgc
     5401 tgactattta aggaaccggc tgttaatcaa aaacccttga cagagtaacc caaaactttt
     5461 attcttagcc atgaagaaac tctggggagg aaggttcagg gaaaagacga gtagcatcgt
     5521 agaagccttt acggaatcca tctcttttga catacgtctc tggaagcacg acatcgaagg
     5581 gagtattgcc cacgccagga tgctggggaa gcaggggata atctccaaga acgattcaga
     5641 aaaaatagtc cgcggactga aagagatcgc aaaggagata gaaacaggaa agttcagatt
     5701 cagggaagac ctcgaggatg tgcatatgaa catcgaggcc gcactcacga aaaagattgg
     5761 ccctgcaggg aaaaggctcc attccgcgcg atcaaggaac gaccaggtcg cgctggacct
     5821 caggctttat ctcagggaag agataggcct gatactttct gccgtcacac gatttcagac
     5881 cagcctggtg aatatcgcgg aaacccatat tgattatatt atgcccggtt acactcatat
     5941 gcagagggcg cagcctgtac tcctttctca ccatctcctt gcctatgtgg aaatgtttga
     6001 gagggacaag gggaggctca gggatgcgtt gaagagggtg aatgtgttgc cccttggagc
     6061 gtgtgccctt gccggtactt cattgcccat cgacagggga tatgtggcga agctcctcgg
     6121 ttttgattct gtcgcggaaa acagtattga tgcggtctca gacagggact tcggcatgga
     6181 attcctctct gcttccgcta tgttcatggt gcacctttcg agaatggcgg aagagctggt
     6241 gctttggtca acagaggagt tttcctttgc tcagctgccg gatgcattca ccactggatc
     6301 gagcatcatg ccccagaaaa aaaaccctga tgtggcggag ctgataaggg ggaaaacggg
     6361 aagggtcttc ggcgggctct tttccctttt caccatgatg aaaggattgt ccctgtcgta
     6421 caacagggac atgcaggaag acaaactacc cctctttgat gcggtggata caataaaggc
     6481 ctgccttgcg gttctcactg agatggttcc cagaatacgg ttcgataaaa agaggatgtc
     6541 cggaaccgct gcccacgcgt attccactgc tactgacatg gcagaatatc tggtcagaaa
     6601 gggcatgagc ttcagagacg cccatggggt gaccggaaga gtagttcttt attgtataga
     6661 gaagaaaaag gccctccatg aactgaaagt tggggagctg caaagattct cccccctgat
     6721 ccagggagac atttaccgct atcttacggt tgaaggttca gtgggatcaa agagatcaag
     6781 gggagggact gcagcaacgg aagtgaagaa acagattacg aggctcaggg gtctcctcac
     6841 ccaatgatct ttcgagtgct ccttccattc ctttttttta tctcgcttgt tattcccgcc
     6901 tgcggcagga aagcccctcc cacattaaag gcctacgaaa aaccgcaagc tccggcaggt
     6961 ctcacagtca tccacaggga ggggaagata cttgtctcgt ggtcttaccc tggcaatctg
     7021 caatcttcca taaaagggtt tcaggtcatg agggcagagg gagaaggatt cgaaaggcgg
     7081 ggatttgtag ggaacgaggg gaactcgttc cctgacgata gcttctctcc tgatactacc
     7141 tataaataca aggtcatggc ccagaaccta aaagatattt tgagtgatga ttcaactatt
     7201 gtgaccataa ctcctaagct gttgcctcct ccgcccgagg atgttcaggt ttcggtggga
     7261 aatgacgctg tccagttgtt gtggcagagt tcgggtgagg gggtctgtta tcatgtatac
     7321 aggacaacag aaaagggcag gtatggcgat gtccctattc acagagaccc tatttgtacc
     7381 acttccttta gcgacagaac gctctttcag gataaacctg tgtattacac cataagggca
     7441 ttgcataaca ctcccctgct cgatgaagga tatccttctg ccgaggtcga ggtcacccca
     7501 tcacatttta tcccctcggc acctgcggag ctcagagggg taagggcgca agggaagata
     7561 gtccttatgt ggagggagag ccctgaaaca tgggtcaagg gataccgtgt ctatcgcaaa
     7621 aaagagaggg aaaaagagtt taccttcctt ggtgaggtaa agacacccac cttctcggat
     7681 acagggaaag tagggaaaaa ggtaaggtat agggtaacgg cattgggacc ttcacaggaa
     7741 agtgaaccct ctgtcactga agtgccctag ctcttataag agaggtatcc gcctctcagc
     7801 aaaacaatag cccttattga ctattcccgg tctaagcacc taagaacacc ctgttcccat
     7861 ttcttgtcag ggtccggaac tgcggaccgg cacataaccc atccgcctca ggcggattgc
     7921 cgttttcgca ccagcgaatc agcccacgct gactagacgg atacctctat cgagcttatg
     7981 atgatgacaa ttctccttga cattaaaata aaccatcggt tagtctagac aaatcctttt
     8041 tccggctgtg aaataattta atcctgcgta aggtttacaa tgccctggat ggaagagaag
     8101 atagtcaagg cctttgagga aagcataagc gtcaagcaaa acttttttaa ggaaaatgtg
     8161 ggagcaatcg tggaggcctc caaaatgatt gcctcggcct ttactgaagg gaaaaaactc
     8221 ctcctctttg gaaatggggg aagcgccact gatgcttcac acatggctgc tgaatttgtg
     8281 aatcgattta aaagggaaag gcctggtctt cccgcgatag ccctcaccac cgacatggcg
     8341 gtcatcacct ccatagccaa tgactatgat tattcagaga tctttgcacg ccagatcaaa
     8401 tccctttcgg atgaagggga tttggtactc gccatcagca ccagcgggag ctcgggcaat
     8461 gtgctgaaag caatggatgt ggcaaagaga aaaaagctga agaccatcgt gttcacaggg
     8521 gccaagggcg agaaatttgc ttcaaaggcc gactgcgcct tcatcgtccc ctcagataat
     8581 actcccaggg ttcaggagac ccatatcaca ctgggccatg tcctctgcca gatggtggag
     8641 gagatactgt ttgagtcgcc aaggaaacga tagcggtatc gccttactca tctctatggg
     8701 tgcagaccgg ggaacctgaa tatgatcatt cttggtatcg atcccggact ggcgagcaca
     8761 ggcttcggtg ccatccaatg cggtaaggcc acttcttcgc tccttacctg cgggtatatc
     8821 aagacatctc ccaggcaaca catttcccac agattgttcc agatccattc tgacgtgaac
     8881 catctcatac gctcattaca ccccgacctg attgccattg agaatgtgtt ttccctggta
     8941 aaatacccga aggcgggtat cttattgggc ggtgttttgg gtatcatata cctgtccgtg
     9001 ttccagaatg atgtctcgat gatggagata acaccaaggg aagtaaagaa ttccctggca
     9061 ggatacgggg gtgcagataa ggatcagatg aagaggacgg tcaagaaact gctcaacatc
     9121 cctgatctta aatccttcca tgcctccgac gctctcgcgg tggcgttaac cgcattttac
     9181 agaaggacat cgtggggggg ccgttgattt cctacctgaa gggaaaagtg agacttaacc
     9241 gttcagatga caagatcacc ctgatcgtga acggtatagg ctacgacata ctgatcccgg
     9301 cgtatgtgat gagcggaatt aaaggaagca gggcggatgg agatgaaatg gaactttatg
     9361 tttcctataa ccagacagaa agacagccga aaccggtact tgtgggattt gaatcgcccc
     9421 tggacaagga attctttgaa ctcttcatat ccgttgagga tattggcccc actgcagcaa
     9481 tcagggccct cacaaaaccg gtgagagaga tcgcccgctc catcgaaggg aaagacacga
     9541 aggcgctgaa ggcactgaag ggaattggtg agaggaaggc agagaagatc atcgcgacgc
     9601 tgaaagggaa ggtggcaaaa tttgcgttga tgcctgaggt tgaagtgccg gtaggggttg
     9661 cggaagattt caggaaagag gtggaagatg tgcttatgaa tcagctgggg cataaaccta
     9721 tggaggcgcg caagatgatc gaggaggcca tgaagcggaa tccccgtatc tgctcgtccg
     9781 aagagctgtt cgaggaagtg tatcgggggc aggggcaatg attcacgaag aaggggatcc
     9841 cctctccgag atcctcaaag aaaacagttc cctggacgaa gacgcgataa ccctcagacc
     9901 cagacggctc acagagtata taggacagga taagatcgta gagacactga agatcgccat
     9961 cgaggcggcg ctgcagagga aggaacccct ggaccatatt ctctttaacg gaccacccgg
    10021 tctgggaaag actacgctgg cccatatcat tgccaacgag atgggcagca agatcatcac
    10081 ctcatccggc cctgccctgg aaaagggcgg ggatctcatg ggtatcctca cccatatgga
    10141 aaagggagac gtgtttttta ttgatgaaat tcacaggatc ccgaaaattg tggaggaatt
    10201 cctctatcct gccatggaag actttgcggt ggattttata ttcgacaaag gggtgcatgc
    10261 gaggacccac cggtacaggc tcgaaccctt caccctcatc ggcgcgacca cgagagcagg
    10321 acttttgtct tcccctctga gagaacgatt tgggatcaca agggacctcg acttctatca
    10381 agacaaggac ctcgttcaaa ttgtgaagcg ctcctccgcc atactggagg ttactatcga
    10441 gcaggatgga tcatatgaga tagcccggag ggcaaggggc accccaaggg ttgcgaacag
    10501 gttgttgaaa cgtgtgcgcg attatgcaca ggtgagggca caggggaaga taacgggaca
    10561 ggtggccaaa gaggcgctgg ctctggaggg agttgatgag gaagggcttg gggagaccga
    10621 ccgaaagctc ctgaaaacca ttattctgaa ttataagggg ggcccggtgg ggattgaggc
    10681 cctggcttct accctccaga tagagacgga cgtgttattg gatgtggtgg aaccgtattt
    10741 attgaagtca ggctttatca taagaacatc ccacggcaga aaggccacgg aaaaggctta
    10801 ccaccttctc aatatccccc ataagggtga gataggatta tttgaagggg caaaaggcaa
    10861 gaaacatcct tagaggttct ccttgggtat ctcagaccat tttatccctc tcccgacctg
    10921 gccggcccag aaggccagag agtttttcca ggagaagaat gaggaagcat acaatcttgt
    10981 tgatgtccgt catcccaggg agtatgaggt cgggcatctt cccggagcgc aattgatccc
    11041 tctcgatgaa ttgccggacc accttggaga tcttgatcct gaaaagctca ccatcactta
    11101 ctgcgcaaag ggcattcgca gccgtgccgc agctgcattt ttgtcaggat caggatttaa
    11161 ggaggtgtac agcattgaag gaggacttga ggcctggcat ggccttgtgg ccgaaggccc
    11221 ccctgattcg gggatggcat atttttcacc ggcagccggt ccgggcgaac tcattgccct
    11281 tgcatggctt ctggaggatg gctcacgcaa attttattat gcattgtcgg agcttcttga
    11341 caataaagac gctacggtcc tttttcgcga actgaccctt gcagaggaac atcacatgtc
    11401 ctctctcctt acactctaca cgcaattttc aggaaaggaa cccgacgcag gatttccatg
    11461 gtcagtgctc tccttggaac ctgagggaga tatcatggaa ggagggatac gtgtggatga
    11521 ggccctgcaa tgggttcagg ggaaagaggt aaaggatatc cttgagcttg ccattgccaa
    11581 cgagacgaac tcctatgacc tctatctcag aatggagcgc actattgaag acacacgttc
    11641 aaaaaagatt tttgggacct tgtccgaaga ggagaaggcc catctccaaa agctgattgc
    11701 gctttctgaa aacagccttt gatatatctt tcaaagactg tcatatatcc acctggtctt
    11761 taccattaag ccctatcagg ggaccacaca aggagattgt gggcgtgagt gtggtctcaa
    11821 aaaacatcac tcaattaata gcctgaaaag acgactttcg tacaggccgc ctgatgatag
    11881 gacggattgg tcatttccac tcactattat tttaacagtg acttgaaaat tttcggaaga
    11941 ttgagcctcc agccttttat gggccagtca agctcgcgct tatcgcagag ggaggcaagg
    12001 ttgttgaggt gaaggatttc tgtccttgtt gcgtcatcta tctgccctga actatcgata
    12061 tgttcaatga ggacctttgc cttctcaata tgctctctta tcatcgatgc tttttctttt
    12121 ccaatgacct gatatttttc ttttatgtcg aggagcatga gggctccgtc atatgtggcc
    12181 ttcacaatat cctcccttgt catccattcc gtctcgtaat tgagggtgta tttccagctc
    12241 ggcatgagca tggcctggcg gtgctcctta agggacttgt agagaaggcg atagccgaac
    12301 ttctcgggct cctcaaagat accgctgccg gggtctacga agggtgcaag gggcgctatc
    12361 atgggaagga gccgcctggt cctgccgaat ttccgcaaaa gctcttcgca gtatcccacg
    12421 gtctccataa ccgaccggta atcctggtaa ggcaggccaa ccatgaagaa tatgtcaatc
    12481 ctcttgcagc cggcgtcgag gagcgttgca atcatctgct ccagctcttt gttttcatag
    12541 tgtttcccga aggccttcct gatctttggg tcgtgtgatt ccggggacac ctcgacatta
    12601 aagttttcga tacaatcagc gacttttctg ataaaggttt cagggggagg ggtgaagaac
    12661 tcgacggtca actcgttttt tatccggtac tttttgactg catccagaaa tcttccggca
    12721 taatcctccc tggcctgcag caggtcccct atcaccatga ccggcgcgcc cgtgtactcc
    12781 gagatctttc tgatatcttc tgcgagaagt tcaggagacc tgaaacatgg tttttctctc
    12841 atgcagaccc tcttgaaaga ggagagggag cccgcgcacg aagcacagtt atggacgcat
    12901 cccctgcatt ggaataccgc ggtaacggga taactgagcc agtaccggaa agggatatac
    12961 cctgacgggt cgcggtattt tatggccatc ttgagaagat ggaggtaatc aaaattgatg
    13021 tcatcgaggt tctccggcct gttggaaagc tcatttgtga tgacctttcc gcttccgtcc
    13081 ctccatacaa gatttggcac gctctcaaag ccggtcccgg attttatcgc ctccatcagc
    13141 atccgcagag gctcttctgc agagtcgccg agcacgatga aatccacgaa gggatagtcc
    13201 gccatgatct caccgtgata ataagtggca gagagcccgc caagaatgac cggcttcccg
    13261 ggatgatgct tctttaacat ttccgcaagg cacaggcttc cgtccgcgtg ggggagccag
    13321 tggaggtcaa taccaaaggc cagcgcatcc agcttcttta tgaatttctc gggattgaag
    13381 gattcagccc tgagcatctt catggcaagg tttacgatcc tcaccggtat tccgtgacgg
    13441 taaaggtagt cggagatagc gagaaaaccg atgggataaa tctcaaagat cgaagaagac
    13501 ggtatcacat cgctcatggg accgtaaaaa cccggtcgct cgcggaatct gaagacagtg
    13561 gggggatgaa ggaggatcag gtcaggtctg ggcctgaaga ggttatgcat tacagtttca
    13621 actcctttat caatcctact cctatttctc ttaacgcatt cttccctttt atctctttca
    13681 gcgagaaggg aagcattgcg accggtaatc catctgcgag ccttatgaga tctgccagat
    13741 atagttcctg cgtttcctgc agggtcctga aggagggaga gtcgggatca ttgattaccc
    13801 tattgataat cataccctgg agggtgatat ggtaccccct gagggtttct accacccttc
    13861 gggcttgttt gaccacaagc gcttcagggt tagcgacaac aacgaaggct gcgctgcgaa
    13921 gaatatatcg tgatatcctc tccgatgcag caatccattc atccatgatt cgcgcgatcg
    13981 acctttttcc tgcgatcttt ttcccgatct tgtccagccc ctcgaaaacc ctggagccag
    14041 agctgagatg gttccttatg taggtcggca tattgaggag attcagtgtt tcgcccgcag
    14101 gtgcagtatc ccagacaata cgatcatagg tgccggtctc agccagatct acaatgaggt
    14161 cgagcatggt ttcttccctc agggaagggg cagaaccgat atagtccagc agctggtggc
    14221 gtgactcgtt gtcaagtccc tccacatcta taaggtgaga gagtatctca taaaaatcag
    14281 ggccgaattt tttcttccac ttgttcagga tggcgtcatc acttatttca acggcgtcga
    14341 gacgctcctc tatggatctg atggtatctc ctatctcttg ttcaaagatg tcggaaaggg
    14401 aaggggttgc atccgacgtg ataatcaagg ttttctgtcc ggatgatgcg agctgcaatg
    14461 cagcggtcgc cgaacaggta gtcttgccga caccgccttt gcctccgaac atgatcatag
    14521 gtctgcaaat caattctttt tccacggcgt ttcattttac ctcatataat gaggaaaagc
    14581 catggtgttc gagtatgttt gcatatcctc cccattcacc ttttgcgaaa ggggagcgaa
    14641 tacgctctag cccccttaca tccctcttcc ggatctcagt ctttccagtt ccttctgaat
    14701 cggccactcg gttaaggcaa accagatgaa aaggatgacg tttactacag ggacgaaaag
    14761 gagaataatc tggaggcctg aatagccggc ctttctgaag atgaggacca tgagcagggc
    14821 ccatataatg aaacctaaca gagcgaaaaa tcccatgcct ccctccttaa tgtggtattg
    14881 agttatattt tagacgagaa aacagtgatt gcaaataggc ggcaagaatc aggacctgaa
    14941 atagattttg tcgaagttca tgatcaggag ggctatagca atttggagga ttattccggt
    15001 gatgagcgaa gagatgaaat taagccgtgg tgaaaggaat atgaccgtca ggatgaaaat
    15061 catccagggg atgatcatga ttatcagtcc ttttgcgtac gccaataccg cagtctggcc
    15121 ggcagtaaaa taaatcaata agaatgtgct tatagttata atggggaggg taccgataaa
    15181 cgcggcgagc aaccccttgg agttgttcgc aagataggtg accgcactcg taattacccc
    15241 ccccaaacag gaaatagagg aaatatttca gtcccatagc attaacatca tcctgattat
    15301 aattgtaaca attaagagca cctatctgct gtgcgggcag gatgaagacc aagagtcttc
    15361 ttaaaccttt tcccctttcc cttgtctaca gattgagaga ggtataatgt taagagcaag
    15421 gtgcattcgt ttccaaggag ggacaatgaa aactattgat caacatagag tggggcggct
    15481 gcttatgcgg tggtccctat cagcagcagt ggccatggct gttcttgctt catacagccc
    15541 tgcccgggcc gactggaact ggccccggga agtccaggtc aaagacggaa ctttgacgat
    15601 ctacacccct cagatcgaag cgttccgggg ggacaaggta gatggccggg cagccataga
    15661 gatggtgccc aaggacggca aggagaccac gttcggggtc ttctggtttt ccgcgcgcct
    15721 ggcaacggat cgtgacagcc gtacagtttc actcgaggac atcaaggtca caggggtgaa
    15781 attcccggaa aaggcagggg cacaagatac tcaagctgcc gaggccatcc tgaggcaggg
    15841 gggcggcctg tcatcgatgt ccatgtccat ggacactttc gtggcaataa cggcggtcct
    15901 gccgcgggag aaggttgcgc ctgagaagtt taataacgcc ccgcccaaga tcatcgtcgt
    15961 caaggtgccg acggcgctgg tcatcatcaa cggcaagccc atcatgaggg atgttcccgg
    16021 cacggccgtc aaccgggtcg tcaacacgcc ttttgtcatg ctgtatgaca aatccgccaa
    16081 agcgtactac ctcaaagggg gtaagtggtg gtatcaggcc accgacatca agggcgattg
    16141 gaaaacaacc gatacgccgc ccgaggccat agtgcaggct gcgctcaagg tatcggagcc
    16201 tgaacccccg gagctcaagg catttacaca ggaggaaggc tctgctgcgc ccgccgtgat
    16261 agtggccacc gagccgacgg aactgatcgt caccgacggc gaccctcagt acgcagtgat
    16321 tcctggtacc gacctcctgt atgtgagcaa cacgggcaac gacatcttca tggacgtcaa
    16381 ttccaaacaa ttctacgtgg ttctggcagg ccggtggtac cggagccctt ccctggcagg
    16441 cggcaagtgg aactacgtgc tatcacagaa gctaccggcg gccttctaca ggataccccc
    16501 gggctctgac aaaggatatg ttcttgcctt catagccgac acgaagcagg ccgacgaggc
    16561 tgtaatggag gcgcagattc cgcagacctc cgcgatcaag cgtagcgagg ccaccgccga
    16621 tgttgcctac gacggcagcc ccgaatttaa accgataaag gatacaggcc tcgaatatgc
    16681 cgtgaacaca ggctcgcagg tgataaaggt caacggcaag tactaccttg cccagaaggc
    16741 cgtgtggtac gtgtcagatt ccccgacagg gccgtgggtg gtctcggacc acactcctcc
    16801 gaatatcgag tccattccgc ctgacagccc tgtttacaac gtgaagtatg tacgcgtgta
    16861 cgatgccacg cccgatgtgg ttcatgcggg ttacacctct gggtacgttg gcagctacgt
    16921 tgacaacggc acggttgtat acgggaccgg ttacgattat cctggatggg aaggtactgg
    16981 gtactatccc gcgcctctta cctggggtct gtccccctgg tattaccctt attacgggga
    17041 gtggggcttt ggggcgggtt ttgtttctgg tgctttcctg ggatttgtta caggcgctat
    17101 cgtgtctccg tggtggggct ggggcggctg gtggggtccc tggtggggcg gctggtgggg
    17161 cccttggtgg ggaggttggc acggaggtcc ctggtcgggc ggccactggg gcggttactg
    17221 gggctggggt tggcacgccc agcagtggcg acacggtaat tacaaaggca cctggcagca
    17281 cggctggcct tccagtaacc tctataaccg gccaggcagc acggcgtgga acacccaggc
    17341 tcacagaggg cctcaggcag gccccggagg cagggccagc ctcatgccta caaacatggc
    17401 ccgggcaagc gcgcaaccca caggctcagg cacaagatac agaaacgtga tgcccggtgg
    17461 cacagcgggt acaaggcccg ggggaggcgc cgcagtgcgg cagaaccggc cttccggcac
    17521 catgcagggt gcacagcgct acggggccgc ccggcagaac aacgtgtttg caagcccgaa
    17581 cggcggcgtc ttccggagaa cgcaacaggg atggcagcag tacaacaggg gcggatgggc
    17641 gcagcctcag tcaggctaca gccgcaacca gtccctcctg aacagggact acaacgcccg
    17701 gatgcgcggc atccagaggg aaagcagctt cggcggctac agtagaggcg gccatagctc
    17761 tggcgtctcc agaggcgggg gattctcatc atctggccgc agcggcggta tgtccggtgg
    17821 aggctcgcac ggcggtggcg taggccacgg cggagggggg aaacggtagc gaaaaactca
    17881 ttgtcctcct atccttgatg acattgacgg gctgttgccc ctgatgccca tcccttgaag
    17941 cgataggtat tcctgttctt ccctagtatt gtcaggaata gcgattagtg ggaagtgtgc
    18001 gacatgtctc caacgggata gccgatatgg cagaccttgc attccttgac ataccttgcg
    18061 catgtgacag aaagacacag tgtggttttc aatgagaatc tacagtcagg gcctgaagta
    18121 gattttgtcg acattcagga tcaggagtgc tatgacgatt tggatgatta ttccaataat
    18181 gagcgaagag ataaagttca attttggaga gaggaatatt accgtcagga tgaaaatcat
    18241 ccaggggatg atcatgatta tcagtccttt tgcgtacgcc agcacagcag tctggccggc
    18301 attaaaataa atcaacaaaa atgtgcttat ggttattatg gggagggtgc cgataaatgc
    18361 ggcgagcaag cccctggagt tattcgcgag ataggtgacc gcactcgtaa ttagccctcc
    18421 gaaaaagaaa tagaggaaat attttagttc catatgatta gctcctcgtt gtaattataa
    18481 caattcagag caactatctg ctgtgcgggc aagatgaagt gaggtaggct gggaaaagtg
    18541 gacaccaaag tcagataaaa taaagcagag acggaggtgt caagatgaaa ggtattccgc
    18601 aaggaaggta cacgaaggag tttagagagg aagcagtgaa ggtggtgtta gatggaggca
    18661 tatcgttacc ggag
//
LOCUS       DATKOU010000065         7456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4702128, whole genome shotgun sequence.
ACCESSION   DATKOU010000065 DATKOU010000000
VERSION     DATKOU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4702128"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VMU21_03100"
     CDS             <1..943
                     /locus_tag="VMU21_03100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUO76541.1"
                     /translation="GFPFDGERILSSDNAVTVREIPRRILIVGAGVIGCEYACIFSEL
                     GAEVTMVEMLSRAVSSEDTEISEYLEKEFRKKKITLMTGVKVSRVQAEDDGMHLFLSD
                     GKELVTDKVLVSVGRSFNSEHIGLERLGVKKGERGEIIVNEKMETNVKGVYAVGDVVG
                     GMLLAHKASREGIVAASNACGTDMTMDYSIVPAAVFTSPEIASVGLREHEAVEKGMNI
                     RTGHFPVRALGKAHATGEISGFFKIITDARTDRIIGAHIIGPHASDLIHEIAVAMKAG
                     LAAKDVAETIHAHPTFSEGIREAAEDILGEAIHVPKK"
     gene            962..2338
                     /locus_tag="VMU21_03105"
     CDS             962..2338
                     /locus_tag="VMU21_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76542.1"
                     /translation="MAFTALSDNWLASERKKHWGIFSELNVLLRALDRFFNIENLPIS
                     KEDVTGGNFFEEISAVRDTILRIIGLIDLVIPESKKNAYWFQKFAHSKFLTDKKRDAL
                     REELYKQDRPEKGLFLLYDSIVNLKGIITDILRTEHISYLTYANIGEMVGRELRENLH
                     FNPFQKDIDPEFDIIENREISQMVKGIREQETRKYVSVILLHLFRFLRYLRYADTSTR
                     FGSLNPSLLIILLLRSEGAMFVMYLDTVSRKGMDEGIRMLMRAMSYQFSMEAKRVFLQ
                     ELKEIFKNRSPQYLKGKIENSHGILKNLAEQSVVQLVSFFNPAIKGSDIFDTFLTKRE
                     QSIRLREDILILERFLALVEKEGTPEKRVHVFDAMRNFMLYFQSFTFKLLRYDDYEEF
                     TSFFQKVFALNREGLKGKGFDKLKDKMRQFRVFIGTCLRQISDRAELADVPVDMKRVE
                     NSLGQYLQ"
     gene            2448..3725
                     /gene="eno"
                     /locus_tag="VMU21_03110"
     CDS             2448..3725
                     /gene="eno"
                     /locus_tag="VMU21_03110"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUO76543.1"
                     /translation="MGEIIEVNAMEILDSRGNPTLQVEVLLDSGVRGKAAVPSGASTG
                     EREALELRDGGTRFHGKGVLNAVNNVMQEIAPKLMGLDSMNQANIDAVMRELDGTENK
                     ARLGANAILGVSLAVCRATALELEMPLYRYLGGCNAKELPVPMMNILNGGAHADNNLD
                     IQEFMIMPAGLPNLSGAVRAGSEVFHSLKAILKKNKLGTAVGDEGGFAPHLKSNEEAI
                     RLIVRAIEDAGYIPGREISLALDVAASEFYSAKGYHFEGKNRAAEEMIDYYEKLMNRY
                     PILSIEDGLSEKDWKGWRRLTQRLGKAVQLVGDDIFVTNTEIIQEGIRKKIANSVLIK
                     LNQIGTLTETLDAIEMSKRAGYTAVVSHRSGETEDTTIADLAVGCNTGFIKTGSLSRS
                     DRVAKYNRLMEIERELSDSALYKGKNALYNLAG"
     gene            3722..4045
                     /locus_tag="VMU21_03115"
     CDS             3722..4045
                     /locus_tag="VMU21_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HUO76544.1"
                     /translation="MNSHQLLRKQVTAELKKRRLIFLTIAILSLLYLVISFTFGETGL
                     LRYLRLRETKSQLVRELHEVETQNSRLQSDTKRLRENPFYLEKHARENFGMAKPDEYV
                     FKYEQ"
     gene            4035..6209
                     /locus_tag="VMU21_03120"
     CDS             4035..6209
                     /locus_tag="VMU21_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HUO76545.1"
                     /translation="MSSSPLSIAFLWHMHQPYYKDPFSGIYRLPWVRLHGTKDYLDMA
                     MLLDEYPHIRQTFNLVPSLIEQIIDYTENNAKDAFLEVTLKRASDLSDEERAFIIGNF
                     FLANWDTMVRPFPRYYELLLKRGLTFSKSDLTRTIRYFSDSDFLDLQVLFNLVWIDPM
                     LRKRDPFLSSLVTKGRGYSEEEKNLLAEKGLSILRDIIPKYRELSSKGQVELSASPFY
                     HPILPLLWDTNVAKVPTPDVRLPKKRFSHPEDALAQIRMAMDFFEGVFGQRPAGMWPS
                     EGSVSEDIVRAIREEGIEWIATDEDVLAGSLGIGLRGPSGNLLRPDLLYSVHEFSGAS
                     LLFRDHKISDLIGFVYSGWEPKRAAEDLIHKFLQIHESLPGNEPHLVPVILDGENAWE
                     YYENDGEDFFRYLYEGLSKYEQLRTTTISGYLGEHRERKKLERLHAGSWIYANFNVWI
                     GHEEDNTAWDYLTETRDALGEFQSMNPGRAMDSAWKALYVAEGSDWNWWYGDEHSTEN
                     AEEFDELFRQNLTRVYREMGREAPPHLFVPVLREDRSVAPSMEIRGFIHPKIDGIMTS
                     YFEWFQGAYLDVGKSGGSMHMTEGLISRIYYGFNQQTLFVRVDPKTSFAEFPMDALLT
                     IEFVRPYPFRVDISIRDTGTAHLLHKSDAGWTGVKELQDIAIGDILECAIPFRDIGAK
                     ERDELNIFLSLRKEEENVERCPWRGYITLTVPTPDFEAMMWY"
     gene            6247..6978
                     /locus_tag="VMU21_03125"
     CDS             6247..6978
                     /locus_tag="VMU21_03125"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HUO76546.1"
                     /translation="MILGVNIDHVATLREARKGSEPDPLMAATLAILGGADGITVHLR
                     EDRRHIKDRDLKLLREMVNTELNLEMAATEEMVRIALEVSPELVTLVPEKRRELTTEG
                     GLDVVRQKEHVKLTVKKLHNSGIPVSFFINPSIMDVDISKETGADMVEIHTGLYSNAK
                     GNKKGKELQRVHEAVRVAHNVGLSANAGHGLNYFNVGRIAEIEGLRGLYIGHSIVSRA
                     VLVGLERAVREMKELIRQSAVSSKS"
     gene            7048..7431
                     /gene="acpS"
                     /locus_tag="VMU21_03130"
     CDS             7048..7431
                     /gene="acpS"
                     /locus_tag="VMU21_03130"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HUO76547.1"
                     /translation="MIYSIGIDIVKVERMREVVGKWEERFLQRIFTPDEIAYCYHKRD
                     PFPSLSVRFAAKEAVIKASGSHVSIPLIDIEVVNDDRGNPTIQPRGALRAFFRENALS
                     KVHLSLSHEKEYGIAFVVLEGQDIC"
BASE COUNT         1989 a         1668 c         2106 g         1693 t
ORIGIN      
        1 aggattccct tttgacggcg agaggatcct ttcaagcgac aatgcggtca ccgtgaggga
       61 gatcccgagg cggatactca ttgtgggtgc cggggttatc gggtgtgaat atgcatgcat
      121 attcagcgag ctcggggcag aggtgaccat ggtggagatg ctttcgcggg cggtttcttc
      181 ggaggatacc gagatatcgg agtatctgga aaaggaattc aggaagaaga agataacatt
      241 gatgaccgga gtaaaggtgt caagggttca ggctgaggat gacgggatgc atttgtttct
      301 ttcagacggc aaagagctcg tgacggacaa ggtcctcgtc tcggtgggaa ggtcctttaa
      361 cagtgagcat atcggtcttg aacgcctcgg agtcaagaaa ggggaaagag gcgaaatcat
      421 tgtgaacgaa aaaatggaga cgaacgtgaa gggggtatac gcggtcggtg atgtggtggg
      481 aggtatgctc cttgcccata aagcatcacg ggaaggtatc gttgctgcat ccaacgcatg
      541 tggcacagac atgactatgg attactccat cgtgcctgcc gcggtattca cctctccaga
      601 gatcgcgtca gtaggactca gggaacatga ggcagtggag aaggggatga acatcaggac
      661 gggtcacttc ccggtccggg cactggggaa ggcccacgca acgggagaga tatccggttt
      721 cttcaagatt attaccgatg cccgcacaga caggataatc ggtgcacaca tcattggccc
      781 ccatgcatct gatcttatcc atgagattgc ggtggcaatg aaggcgggcc tggccgctaa
      841 agacgttgca gaaacgatcc atgcccatcc cacgttctca gaagggatta gggaggcggc
      901 agaagacatt ctcggtgagg ccatccatgt accgaagaaa tgaactgacc ggctaggcgc
      961 aatggcgttc accgcactct cggacaactg gctcgcctcc gaaagaaaaa aacattgggg
     1021 gatattctcg gagctcaatg tccttctccg ggccctggac cgctttttca atatcgagaa
     1081 ccttcccatt tcaaaagagg acgtgaccgg gggaaatttc tttgaggaga tttctgcggt
     1141 gcgggacact attctgagga tcataggcct tatagatctg gttatccccg aaagcaaaaa
     1201 gaatgcttac tggtttcaga aatttgccca ctcgaaattt ctcacggaca aaaagaggga
     1261 tgcccttagg gaggaactct acaaacagga caggcctgaa aagggcctct tccttctcta
     1321 cgactctata gtcaatctca aaggaatcat tacagatatt ctgagaacag aacacatatc
     1381 ctatctcacc tatgcgaaca ttggggagat ggtaggaagg gaactgaggg agaatctcca
     1441 ctttaatccc tttcagaaag atattgaccc cgagttcgac atcatagaga accgggaaat
     1501 ttcccagatg gtgaaaggca tacgcgagca ggagacgagg aaatacgtct cggtcatcct
     1561 gctccacctg ttcaggtttc tgagatacct caggtacgcg gacacctcga cgagattcgg
     1621 ctcgctcaat ccgtcgctcc ttatcatcct gctcctgcgc tctgaaggcg ctatgttcgt
     1681 catgtacctc gataccgtgt cccgcaaggg gatggatgag gggatcagga tgctgatgag
     1741 ggcgatgtcg tatcagttct ccatggaggc aaagagggta tttctccagg aactcaagga
     1801 aatattcaag aacaggtcac cccagtatct caagggcaag atagaaaaca gccacgggat
     1861 actcaaaaac ctcgcggaac agagtgtggt ccagctggta tcattcttca atcctgccat
     1921 caaagggagt gacatctttg acaccttcct tacgaaaagg gaacaatcca tcaggctgag
     1981 ggaagatatc ctcatcctcg agaggttcct cgcccttgtg gagaaagagg gcacgcctga
     2041 gaagcgggtg cacgtctttg atgcaatgag gaattttatg ctctattttc aaagcttcac
     2101 cttcaagctt ctcagatacg acgattatga agagttcacc tcatttttcc agaaggtctt
     2161 tgccctgaac agggaagggc ttaagggaaa ggggtttgat aaactgaaag ataagatgag
     2221 acagttcagg gtattcatag ggacatgtct gagacagatc tctgacaggg cagagttagc
     2281 ggatgttcct gtggatatga agcgggttga aaacagcctt ggtcagtatt tacaatagtg
     2341 caattcactc tcggtgaaga tatcagaata ttcattcaaa atggaagcac tccatgagtg
     2401 tgaaccgtgg gacataaact ttatatactg aatgataagg aggacacgtg ggagagatta
     2461 ttgaagtcaa tgccatggaa atactggatt cgaggggaaa ccccaccctt caggtggagg
     2521 ttctcctgga cagcggagtg cggggcaagg cggctgttcc ttcaggggca tccacaggag
     2581 aacgggaggc cctcgaactg agggatggag ggacacgatt ccacggaaag ggtgtgctca
     2641 atgcggtgaa taacgtgatg caggaaatcg cgccaaaact gatgggcctc gactctatga
     2701 accaggcgaa tattgatgca gttatgaggg aactggacgg gacagagaat aaggcacggc
     2761 tcggcgccaa tgcaattctc ggggtgtctc tcgcggtctg cagggcaact gcactggaac
     2821 tggaaatgcc gctgtatcgg tatctcgggg gatgtaatgc gaaggaactc cctgtgccga
     2881 tgatgaatat tctgaacggc ggagcacatg cggacaataa tctggacatt caggaattca
     2941 tgatcatgcc tgcaggcctg ccgaatcttt cgggtgcggt tcgtgccggc tccgaggtgt
     3001 ttcacagcct aaaggccatt ttgaagaaga acaaattggg caccgcggtg ggcgatgagg
     3061 gagggtttgc tccccacctg aagagtaatg aggaggcgat caggcttatt gttcgcgcca
     3121 tagaagacgc gggctatata ccgggaaggg agatctccct tgccctggat gtggcggctt
     3181 ccgagttcta ttccgcaaag gggtaccact ttgaaggcaa gaaccgtgcg gcggaagaga
     3241 tgatcgacta ctatgaaaaa ctcatgaatc gttatcccat cctttcgatc gaagacggcc
     3301 tttcggaaaa ggactggaaa gggtggagac gtctcaccca gaggctcggg aaagcggtgc
     3361 agcttgtggg cgatgatatc tttgttacca atacagagat cattcaggag ggaatccgga
     3421 agaagatcgc gaattcggtg ctcatcaagc tgaaccagat aggtacgctc accgagaccc
     3481 tggatgccat agaaatgtcc aagagggcag ggtataccgc agtagtctcc cacagatcgg
     3541 gggaaacaga agacaccacc attgcggatc ttgccgttgg ctgtaacaca ggatttatca
     3601 agaccgggtc gctgtcaagg agtgaccggg tcgccaaata caacaggttg atggagatcg
     3661 agcgggaact atcagattca gcactataca aagggaagaa tgcattgtat aatttagcag
     3721 gatgaattcc catcaacttc tcaggaaaca ggttaccgct gagcttaaga agaggcgcct
     3781 tatattcctg acaatcgcga ttctcagtct gctctacctt gtgataagtt ttaccttcgg
     3841 ggagacgggt cttctcagat accttcggtt aagggaaacg aagagtcagc tggtcaggga
     3901 acttcatgag gtggagaccc agaattcccg tttgcagtca gacacaaaac ggctgaggga
     3961 aaaccctttc tatctcgaaa aacatgcgag agaaaatttc gggatggcaa agcccgacga
     4021 atacgtcttc aagtatgagc agtagtcccc tctccattgc attcctctgg catatgcatc
     4081 aaccttatta caaggacccc ttctcgggaa tctaccgtct tccctgggta agactccatg
     4141 ggaccaaaga ctacctcgac atggccatgc tccttgacga atatccccat atcagacaga
     4201 cattcaatct tgtgccgtca ctcatcgagc aaattatcga ttacacggag aacaacgcaa
     4261 aggacgcttt tcttgaagtg acgctgaaaa gggcgtccga tctgagcgat gaagagcgcg
     4321 cgttcatcat cggtaacttc tttcttgcca actgggacac gatggtcaga ccatttcccc
     4381 gttactacga gttgctcctc aagagagggc tcacgttttc aaaaagtgac ctcacccgca
     4441 ccatcagata tttttcagac agtgattttc ttgaccttca ggttctgttt aatctggtat
     4501 ggatagaccc gatgctgagg aaaagggacc cttttctctc ctctctcgtg acaaagggaa
     4561 gggggtacag cgaggaggag aaaaacctcc tggcggagaa aggactgtca atcctgcgtg
     4621 acataattcc caaatacagg gaactttctt ccaaaggcca ggtggaactc tcggcctcac
     4681 ctttctatca ccctatactc ccgctcctct gggataccaa tgtggcaaag gtgcccactc
     4741 cggacgtccg tttaccgaaa aagagattct cccacccgga ggatgccctg gcgcagatac
     4801 gcatggcaat ggacttcttt gaaggtgtgt tcgggcagag gccagcaggc atgtggccgt
     4861 cggaaggctc ggtgagcgag gatattgtga gggcaatccg cgaagaaggg atagagtgga
     4921 tcgccactga tgaggatgtt ctcgccggtt ctctgggaat cggactgaga ggtccgtcgg
     4981 gaaacctcct gaggcctgac ctcctttact ctgtccatga attttcaggg gcttcccttc
     5041 tcttcaggga ccacaagatc tctgacctca tcgggtttgt ctattcaggg tgggagccca
     5101 agagggcagc agaggacctc atccacaaat tcctgcaaat ccatgagtcc cttcccggga
     5161 atgaacctca tctcgtcccg gtgattctgg atggagagaa tgcgtgggaa tattatgaaa
     5221 acgatggcga ggacttcttc cgatatctct acgaaggtct ttcgaagtac gaacagttac
     5281 gtactaccac tatttccggc tatctcggtg agcaccgtga aaggaagaag cttgaacgcc
     5341 tccatgcagg gtcctggata tatgcaaatt tcaacgtctg gatagggcac gaagaggata
     5401 atactgcctg ggactacctg accgagacaa gggacgcgct tggggaattt cagtcaatga
     5461 atccagggag ggctatggat tctgcgtgga aggcactgta tgttgcagag gggagtgact
     5521 ggaactggtg gtacggtgac gagcattcca cagagaatgc tgaggagttc gatgagctgt
     5581 tcaggcagaa tctcacgaga gtgtataggg agatgggacg ggaggcccct ccccatcttt
     5641 ttgtgccggt gctgagggag gacaggtcgg tggctccttc catggaaatc aggggcttta
     5701 ttcatcccaa aatcgacgga atcatgacaa gctatttcga atggttccag ggtgcgtacc
     5761 tggatgtggg caaatcgggc gggagtatgc atatgaccga aggccttata tccagaatct
     5821 actatgggtt caaccaacag acactctttg tccgtgttga tcccaagact tcttttgccg
     5881 agtttcccat ggatgccctc cttaccatag aattcgtcag gccttaccct ttcagggttg
     5941 atatctccat cagggacacg gggacggcac acctccttca caagagcgac gcgggatgga
     6001 caggggtcaa agagctccag gacattgcaa taggggatat ccttgagtgc gctatcccct
     6061 ttcgggatat tggagcaaag gagcgtgatg agttgaatat cttcctctcc ctccgcaagg
     6121 aagaggagaa tgtggagcgt tgcccatgga gggggtatat caccctcacg gttccgacac
     6181 ctgactttga agctatgatg tggtactgag agatagcaaa tccgtgtatg cggaggagga
     6241 gtgacaatga ttctgggtgt aaatatcgac catgttgcca cactacggga agcgcggaag
     6301 gggagcgagc ccgacccact tatggctgcg accttggcaa tccttggcgg tgcggatggc
     6361 attaccgtac acctgaggga agaccgccgc catataaagg accgagacct gaagctatta
     6421 agggaaatgg tgaacacgga gctgaacctc gaaatggcgg caactgaaga gatggttcgt
     6481 attgcccttg aagtttcacc cgagctggtg acccttgtac cagaaaaaag acgtgagctt
     6541 accacagagg gcgggctgga cgtggtgagg cagaaggagc atgtgaagct cacagtaaag
     6601 aaactgcata attcagggat tccggtgagc ttctttatta acccctccat aatggacgtg
     6661 gatatctcaa aggagacggg ggcggatatg gtggaaattc ataccggcct ctattccaat
     6721 gcgaagggaa ataagaaggg aaaagagtta cagagagtgc atgaggcggt gagggttgcg
     6781 cataatgtgg ggttatcagc caatgcagga catggattga attatttcaa tgtgggaagg
     6841 atagctgaaa tagaggggct gagggggctt tatatcgggc acagcattgt ctcaagggca
     6901 gttcttgtgg gacttgagcg tgcggtgaga gagatgaagg aattgatacg acagtcagca
     6961 gtgagcagca agtcatagaa atcactaaac tacagggaat caagggagga ataatccatt
     7021 gaccattgac tgtggacaga agtcgatatg atttatagta tcggcataga cattgtgaag
     7081 gtagagagga tgagggaagt ggtcggaaaa tgggaagaaa gattccttca acgtatcttt
     7141 acccctgatg aaatcgcgta ttgctatcat aagagagacc cattcccttc attgtcggtg
     7201 cggtttgctg caaaagaggc agtgatcaag gcatcaggtt cacatgtatc cattcccctt
     7261 attgatatag aggtggtaaa tgatgacagg gggaacccga caatacagcc caggggtgca
     7321 ttaagggcct tttttcggga gaatgcactc tctaaggtcc atctgagcct gagtcatgaa
     7381 aaggaatatg gcattgcctt tgttgttctg gaagggcagg acatttgtta ggtcaggaaa
     7441 tttatcttcc tgtatg
//
LOCUS       DATKOU010000066        12284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4702211, whole genome shotgun sequence.
ACCESSION   DATKOU010000066 DATKOU010000000
VERSION     DATKOU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4702211"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VMU21_03135"
     CDS             <1..803
                     /locus_tag="VMU21_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO76548.1"
                     /translation="LVKRPAARLQPLDKAKILVAHEEPSIMEKITSILTANGYKVIPA
                     KDGIEAMVNATREMPFVAVLGVSIPKIYGFEVCKRLKGRPETKDMKIILIPSVYDMTR
                     YRREPESLHDADDYIEEHLIEESLIKKIEALKGVSQRESAGPSVDTGKIRREEREITE
                     TEKERATRAKKVLQQPFDETTQKEKGGEIERAKRLARTIIADIYLYSKAKMDDAIRKD
                     SFHKTFASELKEGAKLYEKRVSAEIRGIGDFFNEAINNFIEKRRREIS"
     gene            971..1261
                     /gene="groES"
                     /locus_tag="VMU21_03140"
     CDS             971..1261
                     /gene="groES"
                     /locus_tag="VMU21_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HUO76549.1"
                     /translation="MKFKPLRDRVLVKYSEESERTAGGLYIPDTAKEKPQKGEIVSVG
                     PGRVTDDGKLQPISVKSGDVVLFDKYSGSKVTMDEVEYLIIREEDILGIVEK"
     gene            1290..2924
                     /gene="groL"
                     /locus_tag="VMU21_03145"
     CDS             1290..2924
                     /gene="groL"
                     /locus_tag="VMU21_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HUO76550.1"
                     /translation="MAKQLMFDEEAQRAILSGVALLTKAVKATLGPKGRNAILDKKYG
                     APTITKDGVTVAKEIELKDPWENMGAQLVREVASKTSDVAGDGTTTATVLAHAIYREG
                     IKNVVAGGNPMDLKRGIEKAVEVVLEELKKISKPVVDKKEIAQVGTISANSDPSIGEL
                     IAEAMDKVGKDGVITVEEAKGTATTLDVVEGMQFDRGYISPYFITDPERMECNLEDAF
                     ILINEKKISSMKDLLPILEQTAKMGKPLLILAEDVEGEALATLVVNKLRGTIQVCAVK
                     APGFGDRRKAMLEDIAILTGGTMISEDLGIKLESVKISDLGRAKKITVDKENTTIVEG
                     AGDHAKIQGRVKQIKTQVEETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALLRCIPALRNIKLENHDQKIGISIVMKALEEPI
                     RQIVHNAGVEGSVVVEKVKSSKDANYGFDAQKEEYVDMIKSGIIDPTKVTRTALQNAA
                     SVASLMLTTSVMVADLPEEEKKMPPMPGGGGMGDMY"
     gene            3028..4032
                     /gene="mltG"
                     /locus_tag="VMU21_03150"
     CDS             3028..4032
                     /gene="mltG"
                     /locus_tag="VMU21_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HUO76551.1"
                     /translation="MKKNVIAIAGSFLFVFLVYAAAELIIPLPFGNKPMEFEIKRGTT
                     FRQAVDSLAQKGFVRDKWIFFLLGRSTGIDRKIKAGYYSLWGTMTPLEIFNAIRSGKI
                     IEYEITVVPGDSLREVGEKFSALSMTEGEEFQRLCTNREFLDSLDVDAPSLEGYLFPE
                     TYRFPKGLEMKEALSIMVNRLREKFDDDMTLRAMELGLSERDVLTMASIVEKEAITDK
                     ERPIIAGVYYNRLKKHMLLQADPTAIYGVKSSKETITRQDLLKKTPYNTYVIKGLPPG
                     PIASPGLKSIMAALNPSDVPYLYFVSNNDGTHNFSVTHSNHVEAVKIYRSRKKGKEQG
                     "
     gene            4069..5118
                     /gene="ispG"
                     /locus_tag="VMU21_03155"
                     /gene_synonym="gcpE"
     CDS             4069..5118
                     /gene="ispG"
                     /locus_tag="VMU21_03155"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HUO76552.1"
                     /translation="MIKRKKTRVIHVGQVPVGGAHPISVQSMTKTDTRDVKATVKQIQ
                     SLVMSGCEIIRIAIPDAEAAKALGRIKQLVPIPVIADIHFDWRLALEAIRQGIDGLRI
                     NPGNIGAKWKVAEVVSAASEKAIPIRIGVNAGSLTKKLLEKYGHPTPEALLESAEEHI
                     NILEGLGFKEIKVSLKASSVLTTVDAYRLFSRRFRYPLHVGISEAGPSFTGMIKSSVG
                     LGILLAEGIGDTIRVSLSADPEEEVRVGYEILKSLGLRERGVNLISCPTCGRCEIDIR
                     GLASKVENRLKDIQKPLTVAVMGCVVNGPGEAREADIGIAGGKGFGLLFKKGKVVKKL
                     DEDKLLDALLKEIGE"
     gene            5115..5942
                     /locus_tag="VMU21_03160"
     CDS             5115..5942
                     /locus_tag="VMU21_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76553.1"
                     /translation="MKREGISPLSVRNFQNIIYQNYHENLRKLAWRMTANPYRILVSE
                     IMLQQTQVERVSGKYREFIKTFPSFRSLAKSSLRNILVVWQGMGYNRRAVALKKIAET
                     VISDFKGRLPSSEDELMKLPGIGRYTASAILAFAFNKPTVFIETNIRRVFIHFFFQES
                     ARVRDSEILPLVEKTLDRSNAREWYYALMDFGVMLKTKTENPNKRSAHYRKQSPFQGS
                     NRQVRGMILRLLLMKPDITETEIIRELRSNPEKIKENLAVLQNEGFVWRKGKRFKVR"
     gene            complement(5995..7023)
                     /locus_tag="VMU21_03165"
     CDS             complement(5995..7023)
                     /locus_tag="VMU21_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HUO76554.1"
                     /translation="MKAMVLRKVLNLETDNAPLQLVDMKEPVPGGKEMLVRVSRCAVC
                     HTELDEIEGRTPPLHFPIILGHQIVGRVERTGREAKRFREGDRIGIAWIHSACGECNC
                     CHKGKENLCDNFMATGRDAHGGYAEYTVVHEDFAYAIPQIFSDSEAAPLLCAGAIGYR
                     SLMLAGIEDGENIGLTGFGASGHLVLGILRHKYPRSKVFVFSRTVSERVFAAELGAYW
                     TGDSEEESPETLRAIIDTTPAWKPLVKALRNLESGGRLVINAIRKEEGDKESLLGLEY
                     SRDLWMEKEIKSVANITRKDVEEFLALAAEIPLRPEIQEFTLAEANQALQELKERKIR
                     GSKVLRIE"
     gene            7209..7892
                     /locus_tag="VMU21_03170"
     CDS             7209..7892
                     /locus_tag="VMU21_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76555.1"
                     /translation="MKKVIIAKDIRAILEKKKTFLDRSAVRLFSAPSNEKVLALHRAI
                     KADLIIAKLNMPEMSGETLCSLIRDDEELRHISIIIVCSENESDLARCIQCRANAFIS
                     SPIDTELLLQEAHRLLNIAPRTSYRIPIRVKFFGKSKQRVFTGYTENLSSSGMLLCTM
                     SHLFEGDSVMCSFSVPVSTQITVQAEVVRVLGKDEHNANCYGIKFTDLSASATSAIQA
                     FVKKGSESG"
     gene            complement(7962..8312)
                     /locus_tag="VMU21_03175"
     CDS             complement(7962..8312)
                     /locus_tag="VMU21_03175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76556.1"
                     /translation="MINRRKHKRYIRRCEIGFVSDGVTYRGIASDFSLNGLFIRTNHP
                     LPPDTVLDVTVHFPGGLTSQLTIRVIRAWRTATGRVIGSPIKTFKNGMGVEITKKDIS
                     FLHFIRSILDRSQE"
     gene            8826..9743
                     /gene="panC"
                     /locus_tag="VMU21_03180"
     CDS             8826..9743
                     /gene="panC"
                     /locus_tag="VMU21_03180"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HUO76557.1"
                     /translation="MGHFILRNDDTMIEGITGERAMEIIRIPRIMLDIARTHKMHNKT
                     IGFVPTMGALHEGHLSLVRMAKAENKITVVSIYVNPKQFGPTEDLSHYPRDIEGDAEK
                     LTREGIDILFIPVDSLVYPEGFSTRVEVEGLSRKLCGAYRPGHFSGVATVVTKLSNLV
                     LPTRAYFGQKDFQQTVIIKRIVKDLDMGIDIIVCPTVREEDGLAMSSRNLYLTREQRD
                     SAAIIYKCLTEASEAIKSGIIKVTELKKLMGKRLVSEPLVSEIQYCSAYDPVTLDELE
                     QIGNEALLAIALRFGETRLIDNILVAAGQ"
     gene            9860..10159
                     /locus_tag="VMU21_03185"
     CDS             9860..10159
                     /locus_tag="VMU21_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76558.1"
                     /translation="MEDVILDAEEFEEPIKNKLLADELRKEMSYSYPTVVSVEYIDLF
                     MGNDMDFPEIRELLRQGAINAPVILINGIPKIHGGIPRAVIREEVDKLISEGPVH"
     gene            complement(10251..12125)
                     /locus_tag="VMU21_03190"
     CDS             complement(10251..12125)
                     /locus_tag="VMU21_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HUO76559.1"
                     /translation="MEIKLISGKTLSVSEGESLYHALKRQGIYLVSSCGGKGTCGKCR
                     VRVVEGRYEAVSFGNLTQKDKDAGTVMACQTFPLENILVEIPRQSRLIVGDKIAISKS
                     RDLFELLESFDVGILPVVRHLCVNVPPPTLHDNISDIERIRRSLAKAGIKGMRFSHGF
                     VSSMSEALRAAGWKIVLGYTDDLEAVFISNADEYQDMYGLAVDIGTTTVVVYLVDLAD
                     GRLVDVGSTYNSQMRYGDDVITRIVHAAEGGGLNELRDAVVADINDLLMPLLERHTIH
                     RENIETVVIAGNTTMSHLFWGLNPASIREEPYIPTVSFFPKWRSGTAKMAVNPQAPVY
                     TVPSVASYVGGDIVAGVLASKMHRNPEIALFMDIGTNGEIAVGNNEWLMTAACSAGPC
                     FEGSGIKHGMRATEGAIESVKINRETYEPELGVIGDSSPVGICGSGMIDAISEMFLSG
                     LTDLKGKLVRTKTDRIREQDEGMEFVLSRGIEKDVVLSEADIENILRAKAAIYAGVSL
                     LLKEVGFGLDAIEKVYIAGGFGNYLNVEKAIILGMLPDLPREKFTFLGNTCITGAYLC
                     LLSHELRVEAEDIASKMTYMELSVSRGFMDEYMSALFLPHTDMALFPTVAGLMEKKGN
                     "
BASE COUNT         3455 a         2895 c         2927 g         3007 t
ORIGIN      
        1 ttctggtaaa aaggcctgca gcccgcctac aacctctgga taaagcaaaa atccttgtag
       61 ctcatgaaga gccttctatc atggaaaaga tcacctctat cctcacagca aacggctata
      121 aggtcattcc tgcaaaagat ggcatagaag ccatggtaaa tgcaaccagg gagatgccat
      181 tcgttgcggt gctgggtgta tccattccaa agatttacgg atttgaggta tgcaagaggc
      241 tgaaggggag accggagaca aaggacatga aaatcatcct tatcccctca gtgtacgata
      301 tgacgagata ccggagggag cctgaatccc ttcatgacgc agatgactac atagaagagc
      361 acctgataga agagtctctc atcaagaaaa tagaagccct aaaaggggtt tcccaaagag
      421 agtccgcagg tccctccgtg gacacgggca agatccgcag ggaagagcgc gagatcacgg
      481 aaaccgaaaa agaaagggct acccgggcaa aaaaagtgct tcagcaaccc tttgacgaga
      541 caacccaaaa ggagaaggga ggtgagattg agcgtgccaa acgtcttgca agaactataa
      601 tcgctgatat ttacctctat agcaaggcaa agatggacga tgccataaga aaggactctt
      661 tccacaaaac gtttgcctct gaattgaaag aaggggctaa gctttatgaa aaaagggtat
      721 ccgccgagat aaggggcatt ggagactttt tcaatgaggc aataaataat ttcattgaaa
      781 agagaagaag agaaatatct taatattctt caaaaaataa agaatttaac acgaataatc
      841 atttttcccc ctatttacct ggttttatgc catattggga ccttgccatt gatgcactca
      901 aactattata ttagcactca ctagcctaga gtgctaacaa taaaccattt cagagaaagg
      961 agacatgtct atgaagttta aaccgttaag agacagggta ttggtaaaat attccgagga
     1021 gtctgaaagg acagcaggcg gactttacat ccccgatacc gcaaaggaga agccacagaa
     1081 aggtgagatt gtgtcggtgg gtccgggcag ggtgacagat gatggaaaac tccaacctat
     1141 ttcagtaaag agtggtgacg ttgtgctttt cgataagtat tcgggttcaa aggtcaccat
     1201 ggatgaggtt gaatacctga tcatcaggga agaagatatt ttgggaatag tagagaaata
     1261 aagtcttaag atctataagg aggatatcaa tggcgaaaca actcatgttc gatgaagaag
     1321 cacagagagc tatactaagc ggcgttgccc ttttgaccaa agcggtgaag gcgaccctcg
     1381 gtccaaaggg aaggaatgcg attctggaca agaaatatgg ggcacccacc attacaaaag
     1441 acggtgtcac cgttgccaag gagatagaat taaaggaccc ttgggagaat atgggcgcac
     1501 agctcgtgag agaggttgcc tccaagacat ctgacgtggc aggagatgga acaacgaccg
     1561 cgacagtcct cgcccacgct atttatcgtg aagggattaa gaatgtggtg gcaggcggaa
     1621 acccaatgga cctgaagagg ggaatcgaga aggcagttga ggttgtcctg gaagaactga
     1681 aaaagatcag caagcctgtg gtggataaga aagagatagc gcaggtggga accatctctg
     1741 ccaatagtga tccctccata ggggaactca ttgcagaagc catggataag gtaggaaaag
     1801 acggtgtcat taccgtggaa gaagctaagg gcactgccac cactctggac gtggttgagg
     1861 ggatgcagtt cgatcgtggt tatatatccc cctactttat taccgatcct gagaggatgg
     1921 aatgcaacct ggaagatgct tttatcctta ttaatgaaaa gaagatatcg agcatgaagg
     1981 acctccttcc cattctcgag cagaccgcaa agatgggcaa accccttctt atccttgccg
     2041 aggacgtgga aggcgaggcc ctggcaaccc tcgtggtaaa caaacttcgc ggtaccattc
     2101 aggtatgcgc agtgaaggct cctggatttg gagacagaag gaaagcgatg ctggaagaca
     2161 tcgcgatcct taccggcggt accatgatct cggaagacct cgggataaag cttgagagtg
     2221 tgaaaatctc tgacctcgga agggccaaga agatcacggt tgacaaggaa aataccacta
     2281 tcgtggaagg cgctggcgac cacgcaaaaa tccaaggcag ggtgaaacag ataaagacac
     2341 aggtcgaaga gaccacctct gattatgacc gtgagaaact tcaggaacgc cttgcaaaaa
     2401 ttgtaggcgg agtagccgtc atcaatgtgg gtgccgcaac agaaacggaa atgaaagaga
     2461 agaaggcaag agttgaagat gccctccacg caacaagagc agcagtggaa gagggtatcg
     2521 tgcccggtgg tggagttgca ctcctgaggt gtattcctgc attaaggaat atcaaactcg
     2581 aaaaccatga ccagaagata ggaatctcca tagtgatgaa ggccctggaa gagcctattc
     2641 gccagatcgt acacaatgca ggggttgagg gctctgttgt cgtcgaaaag gtgaagagtt
     2701 caaaggacgc gaattacggc tttgacgctc aaaaggaaga gtatgtggat atgatcaagt
     2761 cagggatcat agaccctaca aaggtcacca gaaccgccct ccagaacgca gcttctgttg
     2821 catctctgat gcttaccact tcagtaatgg tcgcagacct tcctgaagag gaaaagaaaa
     2881 tgcctccgat gccaggtggc ggcggtatgg gagatatgta ctaaaaagat accaatgcat
     2941 gtctcggggc atgagtacca tgccccgaga gttcctttaa ccaaatttct ttacatttca
     3001 ctcttccttt tggcatccta atttaagatg aagaaaaacg taatcgcgat cgcaggctcc
     3061 ttcttgttcg tctttcttgt gtatgccgct gctgagctga taatccccct tcccttcggt
     3121 aataaaccaa tggaattcga aatcaaaagg ggaacaacct ttaggcaggc ggtggattcc
     3181 ctggcacaga agggttttgt gagggataag tggattttct tcctcctcgg gaggagcaca
     3241 ggaatagacc gtaaaataaa ggctggttac tattccctct ggggaaccat gaccccactg
     3301 gagatcttca atgcaatccg gtccggaaag attatagagt atgaaatcac tgtagtgccg
     3361 ggagattctc ttagggaggt cggagaaaaa ttttctgccc tttccatgac cgagggagag
     3421 gaatttcagc ggctctgcac gaatagggaa ttcctcgact ccttggatgt ggatgccccg
     3481 tctttggaag gctatctctt ccctgagacg tacagattcc ctaagggtct tgaaatgaaa
     3541 gaggcgctat ccataatggt gaacaggttg agggagaagt tcgatgatga tatgacgctc
     3601 cgcgcaatgg aactgggcct ttcagagcgg gatgtgctca ctatggcgtc catagtggaa
     3661 aaagaggcca tcactgacaa agaacgcccc ataatcgctg gggtatacta taacaggctg
     3721 aagaagcata tgcttctcca ggctgacccc accgccatct acggggtaaa gagttcaaaa
     3781 gagacgataa cgaggcagga tctattaaag aaaacgccgt acaacaccta tgttatcaaa
     3841 gggttgccgc ccgggcctat agcatcgccg ggacttaaat ccatcatggc tgcccttaac
     3901 ccatcagatg tgccttatct ctactttgtc tctaataacg atgggactca caatttttct
     3961 gtaactcact caaaccacgt ggaggcggtg aagatataca ggagcagaaa aaaaggaaag
     4021 gagcaaggat aggatgctct ttccgaatca ataaaggata gccataagat gataaagaga
     4081 aagaaaacaa gggtgataca tgtggggcag gttcctgtag ggggtgccca tcccatttcg
     4141 gtccagtcta tgacaaagac agacacaagg gacgtaaagg ctacggtgaa acagattcaa
     4201 tcccttgtaa tgtccggttg cgaaattata aggattgcga tacctgatgc ggaggcggca
     4261 aaagcccttg gcaggataaa acagttggtt ccaatcccgg ttattgctga catacatttt
     4321 gactggagat tggctctgga agcaataaga cagggtattg acggactgag gatcaatcct
     4381 ggcaacatcg gcgcgaaatg gaaggttgcc gaggtcgttt ctgcagcgtc tgaaaaggct
     4441 attcccataa gaatcggggt aaatgcaggt tcccttacta agaaactgct tgagaaatat
     4501 ggccacccaa ctcctgaggc ccttctggaa agtgcggaag agcacataaa tatcctggaa
     4561 ggattaggat ttaaggagat aaaagtttcc ctcaaagcat ccagtgtgct caccactgtg
     4621 gacgcataca ggctcttttc ccgcagattc aggtaccctc ttcacgtagg catctccgag
     4681 gcaggtcctt ccttcacagg gatgattaag agctctgtgg ggctagggat actcctggct
     4741 gaagggatag gagataccat aagggtctcc ctctctgcag acccagagga ggaggtaagg
     4801 gtcgggtatg aaatcctcaa atccctggga ctcagggaaa gaggggtcaa tcttatttca
     4861 tgtcctacct gcggcaggtg tgaaatcgat atcagaggac tggcatcaaa ggtggaaaat
     4921 cgcctgaagg acatccagaa acctttgacc gttgcggtaa tgggctgtgt ggtaaacggt
     4981 ccgggagagg cgagagaggc cgacataggg attgcagggg gcaaaggctt cggacttctc
     5041 ttcaagaaag ggaaagtagt gaaaaaactg gatgaagaca aattgctcga cgcactccta
     5101 aaagagatag gagagtgaaa agagaaggta ttagtccgct ttcagtccgg aatttccaga
     5161 acatcattta ccagaactat catgaaaacc tccgcaaatt ggcctggagg atgaccgcaa
     5221 acccctatcg tattcttgta tcggagatca tgcttcagca gacacaggtg gagcgagtat
     5281 ccggaaaata cagggaattc atcaaaacat tccccagttt cagatccctg gcaaagtctt
     5341 ccctccgcaa cattctcgtg gtatggcagg gaatgggata taaccggagg gcagtggctt
     5401 tgaaaaaaat cgccgaaacc gtcatctctg atttcaaagg gagactccct tcctctgaag
     5461 atgagctcat gaaacttccg gggatcggga gatataccgc ttctgcaatt cttgccttcg
     5521 cattcaacaa accaacagta ttcatcgaaa caaatatacg aagggtcttt attcactttt
     5581 tctttcagga gagcgcgcgt gtgagggatt cagaaattct tcctctggta gaaaagacct
     5641 tagatcgttc caacgcccgg gaatggtatt atgcccttat ggatttcggg gtgatgctta
     5701 agacaaagac cgaaaatccc aataaaagga gcgcccatta caggaaacaa tccccttttc
     5761 aaggctccaa cagacaggtc aggggaatga tacttaggct tcttttgatg aagccggaca
     5821 tcacggaaac ggaaataatc cgtgagctca gatccaatcc tgaaaagata aaagaaaacc
     5881 tcgctgtact acaaaatgag ggttttgtat ggagaaaagg gaaaaggttc aaagtacgtt
     5941 aagcgatgtc tgggaatccc tcattggagc attgcatgca tcgggaaaag gcccttactc
     6001 aatccgcaaa accttggaac cgcgaatctt tctttcctta agctcctgta atgcctggtt
     6061 tgcctctgcg agggtaaact cctgaatttc tggcctcaga gggatctcag cagcaagggc
     6121 caggaactcc tcaacatctt ttcttgtaat attggccacg ctctttattt ccttttccat
     6181 ccagagatcg cgggagtact ctagtccaag gagagattct ttgtcccctt cctctttcct
     6241 gattgcattt atgaccagtc tgcctccgct ctccagattc cttaatgctt ttacgagcgg
     6301 cttccatgcc ggcgtggtat cgatgattgc gcgaagtgtt tcgggtgact cctcctctga
     6361 gtctccggtc cagtatgccc ccagttcagc agcaaaaacc ctctctgata cggttctcga
     6421 aaacacaaag actttggagc gaggatattt gtgtcgcaga atacctaaca caaggtgacc
     6481 agatgcaccg aatcctgtga gcccgatatt ttccccatcc tctattcctg ccagcatcag
     6541 ggagcggtag ccgattgcac ctgcgcacaa aagcggcgct gcctctgagt cagaaaaaat
     6601 ctggggtatt gcatacgcaa agtcctcatg gacaacggta tattctgcat atcccccgtg
     6661 ggcatcccta cctgttgcca tgaaattatc acataaattc tccttccctt tgtgacagca
     6721 gttacactct ccgcatgcag agtgtatcca ggctataccg atcctgtctc cctctctgaa
     6781 cctctttgct tctctccctg ttctttccac tcttccgaca atctgatggc ctaaaataat
     6841 ggggaagtgc aagggagggg tcctcccctc gatttcgtcc agttcagtat gacacactgc
     6901 acatcgtgaa acccttacca gcatttcctt tcctccaggg acaggctcct tcatgtcaac
     6961 aagttggagg ggtgcattat ccgtttcaag atttaaaact tttctgagca ccattgcctt
     7021 cattggtctt caccctaagc cttaatgata tctattctcg tataaaaccg ggactatcac
     7081 aagcaccatt gctgccattt atcttctcca ttgaaatacc tttctgacag atttaattcc
     7141 atgaaggaat tgtacctgaa tatttacagc caccacctcc tccatatata atgataatgg
     7201 tatttgttat gaaaaaggta attatagcca aagatatcag ggcgatcctc gaaaagaaaa
     7261 aaacttttct cgacagatca gcggtaagat tattttcggc gccttccaat gagaaggtcc
     7321 tcgcactcca cagagccata aaagcagatc ttattatcgc aaagctcaat atgccggaaa
     7381 tgagcggcga aacactctgc tctcttatcc gcgacgatga agaattacgg catatctcca
     7441 ttattatcgt ctgttcggag aacgagtctg accttgcacg atgcatacag tgcagggcga
     7501 atgcgttcat ctcaagcccc atagacaccg agcttctgct gcaggaagca cacaggctcc
     7561 tcaacattgc cccgagaacg tcatacagga ttcctattcg tgtgaaattt tttggaaaat
     7621 caaaacaaag ggtcttcact ggttacacag aaaatctgag ttcctcaggc atgctcttat
     7681 gtacgatgag tcaccttttt gaaggcgatt ctgtgatgtg ttcattctct gttccggtgt
     7741 caacgcagat cacagtacag gctgaggttg tacgcgttct cggaaaagat gaacataatg
     7801 ccaactgtta tggcatcaaa ttcaccgatc tcagtgccag cgccacttct gccattcagg
     7861 cgttcgtaaa gaagggttcc gagagcggat agaaagcgct catcaagttt tttcagtaag
     7921 tatcggtgat aattccggca gccttactcc gacacagata gctactcttg ggatcggtca
     7981 agtattgacc tgataaaatg aaggaaactg atatcctttt ttgtaatctc tactcccatc
     8041 ccgttcttga aagttttgat cggacttcct atcactcttc ccgtagctgt tctccaggcc
     8101 cttatgaccc ttatggtcaa ttgagaggtc aaaccaccag ggaaatgcac tgtgacatcc
     8161 agtaccgtat ccggggggag gggatggttg gtccttataa aaaggccatt cagagaaaag
     8221 tcgcttgcta ttcccctgta ggtgacacca tccgaaacaa atccgatctc acatcgccga
     8281 atatatcttt tgtgctttct cctatttatc actctttaat ccttttctgc tgtagtgaca
     8341 tttctcaagg cgcaaagctc actcttttcc tcgcgacgaa tccaagttac aaggcaattt
     8401 ttgtatgaat tatccatgag caggcgcctc ttgtcaagaa gttcctggcc ttttcaggac
     8461 attatccccg tgcccgccct tgcagtcata ggcggcatta ataagcccca gggtgttcag
     8521 cttatgatgg ggaccatgcc tttttccagc tgtctcttga caatatcgat cttcggatat
     8581 ttaccggtct taatgacatc tctatgactg tcccgaccat aggagaaaca tcatgtgatg
     8641 acattaaaca gtctccagcc atgcttaatt ccatgtttga caaaattatg tctctttata
     8701 tagcttatat caaaaatcag tctgtcttgc tgcgtacggt gtcggcattt ctgtcattgc
     8761 aaggctgttc cggcaggatg gtttcatctg gagattgaat tcctcaggac ggctcctgct
     8821 tgggaatggg gcatttcatt ctccgcaatg acgacacgat gattgaagga atcaccgggg
     8881 agagggcaat ggagattatt cggataccac gaatcatgct ggacatcgca agaacgcata
     8941 aaatgcataa caaaaccatt gggtttgttc ccaccatggg tgcccttcat gaggggcacc
     9001 tcagtcttgt gagaatggca aaggcggaaa acaagattac agtagtaagc atctatgtga
     9061 acccaaagca gttcggtccc actgaggacc tctctcacta cccacgagat attgagggag
     9121 atgcggagaa gctcacgagg gagggtatcg atatcctctt tatcccggtt gattcactcg
     9181 tgtatcctga gggattctcg acccgtgtgg aggtggaggg gctttcaaga aaactctgtg
     9241 gagcttacag gcctggccat ttcagcggcg tggccactgt ggttacaaaa ctctccaacc
     9301 ttgttctgcc tacaagggca tactttggcc aaaaggattt ccagcagacc gtgatcataa
     9361 aacggattgt gaaagacctg gatatgggta ttgatataat agtatgccct acagtccgtg
     9421 aagaagacgg actcgcaatg agttcaagaa atctctactt gacgagggaa cagagggatt
     9481 cagctgccat catttataaa tgtcttactg aagcatccga ggcgataaaa tctggtataa
     9541 taaaggtaac cgagcttaag aagctcatgg gaaaaaggct ggtatcggag cccttggtct
     9601 ccgaaataca atattgttcg gcatacgacc cagtcaccct ggatgagctg gaacagatag
     9661 gcaacgaggc cttactcgcc attgctctta ggtttggcga aacgagattg atcgataata
     9721 ttcttgtggc tgcgggacaa tgatcttcat cgccctgacc cctcttacct aaaatatttt
     9781 tacaggaggg acaagataag gaggcatcta cgtaagcatt atgctacagc gtgtttatat
     9841 acagattttg gataatcctt tggaggacgt tattctggat gctgaggaat ttgaagaacc
     9901 cataaaaaat aaactcctcg ctgacgagtt gcgcaaagag atgagctatt cctatcccac
     9961 cgtggtatct gttgaatata tagacctgtt catggggaat gatatggact tccccgagat
    10021 aagggaatta ctcagacagg gtgcaatcaa tgcccccgtc atcctcataa atggcattcc
    10081 caagatccat ggagggatac cgagagctgt gatacgcgaa gaagtggaca agcttatttc
    10141 agagggacct gtacattaac gaaccaggaa cggattcagg ttgagattga ggcaaagaca
    10201 gttcagttcg ccgttgattg caacctttcg gtccatcctg tagtcagtac ttaattccct
    10261 ttcttctcca taagccctgc cacagtggga aacagcgcca tgtccgtatg tgggagaaaa
    10321 agggcagaca tatactcatc cataaaaccc ctcgacactg aaagctccat gtaggtcatt
    10381 ttagatgcaa tgtcttctgc ctccacgcgg agctcgtgag aaaggaggca taaatatgcc
    10441 cccgtaatgc aagtatttcc gaggaaagtg aatttttccc ttggaagatc cgggagcatc
    10501 cccaatatga tcgccttctc aacattgagg tagttaccga acccccctgc aatatacacc
    10561 ttttctattg catcaagacc aaaaccaacc tccttaagga gcagagatac ccctgcatat
    10621 attgccgcct ttgccctgag gatattctct atatcagctt cggagagaac cacatccttc
    10681 tcaatacccc gcgagagcac aaattccata ccctcatcct gctcccttat cctgtccgtc
    10741 ttggtccgta ccagttttcc tttcaggtca gtgagtccgg agagaaacat ctcggatatc
    10801 gcatcaatca ttcctgaacc acatatgcca acagggcttg aatctcctat gacacccagt
    10861 tctggctcat aggtctctcg gtttattttt accgactcaa tggcaccctc agttgccctc
    10921 atgccgtgtt ttatcccgct tccctcaaaa caggggcccg cggaacatgc ggcggtcatg
    10981 agccactcat tgttccctac cgcaatctct ccatttgtac ctatatccat gaaaagggca
    11041 atttcggggt tcctgtgcat cttggaagca aggacccctg caacaatgtc gccaccgaca
    11101 tagctcgcaa cactgggaac ggtatacacg ggtgcctggg gattgacagc cattttggca
    11161 gtcccactcc tccatttagg gaaaaaactc actgtcggga tatacggctc ctccctgatc
    11221 gatgcaggat taagtcccca gaagagatgt gacatggtgg tgttgccggc aattactacg
    11281 gtttcaatgt tctccctgtg tatggtgtgc ctttccagca gaggcattaa gaggtcgttt
    11341 atgtcagcga ccaccgcatc cctgagctca ttaagccctc ccccttccgc tgcgtggaca
    11401 atacgggtaa taacatcatc accatatctc atctgggagt tatatgtgga gcccacatcc
    11461 actagccgtc cgtcagcgag atcgaccaga tacaccacca ctgtggtcgt accgatgtcc
    11521 actgcgaggc cgtacatatc ctgatattca tccgcatttg aaataaatac tgcctccagg
    11581 tcatcggtat accccaggac gatcttccat cctgcggccc tgagagcctc tgacatggat
    11641 gagacgaacc catgggagaa tctcatccct tttatgcccg ccttcgccag ggacctcctg
    11701 atccgctcaa tatcacttat gttgtcatgg agagtaggcg gaggaacatt cacacagaga
    11761 tgtctcacta ctggcaatat cccgacgtcg aaggattcca aaagctcaaa gagatccctt
    11821 gatttggata tagcaatctt gtcacccact ataagcctag attgtctagg tatttcaaca
    11881 agaatattct ccagggggaa tgtctggcat gccatcactg ttccggcatc cttgtccttt
    11941 tgagtgagat tcccgaagga caccgcttca tagcgacctt caaccaccct caccctgcat
    12001 ttcccgcacg tgcctttgcc accgcaggaa gaaacaagat agatcccctg ccttttgagg
    12061 gcatgataaa gactctcccc ttcggagacc gacagggtct tacctgatat gagctttatt
    12121 tccatatgga cttgatcatg gcacaaacct gcctatgtct tctctctcat cttcatctac
    12181 gacgatgact attatacatc attgcattat gcgcatgtga aaaaacgaga caccatatgc
    12241 cgaaggttct ggttgtcctg aatagcaatc tgctaaggat aact
//
LOCUS       DATKOU010000067        28538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3542158, whole genome shotgun sequence.
ACCESSION   DATKOU010000067 DATKOU010000000
VERSION     DATKOU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 28538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28538
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3542158"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..422
                     /locus_tag="VMU21_03195"
     CDS             54..422
                     /locus_tag="VMU21_03195"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HUO76560.1"
                     /translation="MPYGIFANGVVFIHFLWILFLIFGTCWGVKNRAVRIFHISGLVF
                     AVVIQLFDWYCPLTHLEAWLRSKQSLTTAHPSSFIIYYVQKIVYVELSRSMVIVLTFF
                     LCGFSAWLYLQKRKKPNNPL"
     gene            511..975
                     /gene="ribE"
                     /locus_tag="VMU21_03200"
     CDS             511..975
                     /gene="ribE"
                     /locus_tag="VMU21_03200"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HUO76561.1"
                     /translation="MKVIEGELQAEGLKFGIIVSRFNDFITSKLLDGALDALRRHGAS
                     EKDIEVVKVPGSFEIPMVAHKLAAKGTYHAIICLGTVIRGATPHFEYVSAEVSKGIAS
                     ASMETGIPIAFGVITSDTIEQAVERAGTKSGNKGWDAAITAIEMAQVMKKLA"
     gene            972..1391
                     /gene="nusB"
                     /locus_tag="VMU21_03205"
     CDS             972..1391
                     /gene="nusB"
                     /locus_tag="VMU21_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HUO76562.1"
                     /translation="MKRRKGREYAFQFLFQFDFTGARPEKKDFDLFWADKNGDADLRR
                     FTEDIVSGTLHNLPEIDAAIQKAAEHWVLQRMAAVDRNVLRFAAYELLYRSDIPSAVT
                     INEAIEIAKRYSTNESASFINGILDTIAKGIEGKRMR"
     gene            1391..2116
                     /locus_tag="VMU21_03210"
     CDS             1391..2116
                     /locus_tag="VMU21_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUO76563.1"
                     /translation="MRYAVISDVHANLEALEAVLRDINKRGIDEFLFLGDAVGYGPNP
                     NECVELLRKNCKILLAGNHDWAAIGLTDIQYFNEYAKAAILWTREVLTPESRKFIETL
                     PVTKNIKRGNMLLVHSTPKDPEAWHYLLTLWDAEVNFHYFTHRVCMIGHSHLPFVIER
                     VPSGEMITYKSEARLGKNERYIINAGSVGQPRDRDPRACYAIFDDDTLEFVRLEYAIE
                     KTQKKMRDAGLPLTLIERLSKGT"
     gene            2278..3582
                     /gene="purB"
                     /locus_tag="VMU21_03215"
     CDS             2278..3582
                     /gene="purB"
                     /locus_tag="VMU21_03215"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HUO76564.1"
                     /translation="MIPRYTRPEMGRIWTLENRYMKWLEVEIAACEAWASVGEIPKSA
                     LQTIRKKARFDVDRIDEIEKTVRHDVIAFLTSVAENVGHESRFIHKGLTSSDIVDTAQ
                     SLLMREASDIIIKDIKGLLSVMKKQAFRYKDTPCMGRSHGVHAEPMTFGLKFALWYED
                     MRRNLERMEAARERISIGKLSGAVGTFSSIPPVIEEKVCKKLGLTPEPIATQVIQRDR
                     HAEYLMTLALIGASVERIAVEIRHLQRTEVLEAEEPFEAGQKGSSAMPHKRNPVGSEN
                     LSGLARVLRANALSSLENIALWHERDISHSSVERITIPDSSLLADYMLDRLRTILDGL
                     QVYPERMKQNIAASYGLYNSQRVMLALTEKGLSRESAYAIVQRNAMKSWKEKKEFRKL
                     LLGDKEVRRYLTPKMMDELFSLSHYFKNVDYIFGRVFGRRRA"
     gene            3579..3764
                     /locus_tag="VMU21_03220"
     CDS             3579..3764
                     /locus_tag="VMU21_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76565.1"
                     /translation="MKARLLLTLDTASVEETQRLLSTLGEGLIASGSICDYQFEIETD
                     QGIVTEKCILSGDKVIA"
     gene            3876..7868
                     /gene="purL"
                     /locus_tag="VMU21_03225"
     CDS             3876..7868
                     /gene="purL"
                     /locus_tag="VMU21_03225"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HUO76566.1"
                     /translation="MISLYRKPVLSETKKNSLLSLVREKVSREVMDIQTEYCFYVESH
                     EPLTPGEKDTLQWLLRETFEPHNLSSESFLDNGQTILEVGPRMNFTTAWSTNAVSICN
                     GCGLSKVSRIERSRRYQLVLYRQRGEELKKGLEGLVHDRMTECPYRERLTSFESGIHP
                     EPVYEVRILEEGKSAIEKINRELGLGLDEWDINYYYFLFAKDMKRNPTNVECFDLSQS
                     NSEHSRHWFFKGKLVIDGRAVPHTLMEIIKEPYRRNPNNSIIAFSDNSSAIRGYRIRT
                     ILPEKPGRPSPFTEKEKLYHLILTAETHNFPSGVAPFPGAETGTGGRIRDVQAVGRGG
                     LVVAGTAAYCVGNLRIPGYKLPWEDESFAYPENLATPLQIEIEASNGASDYGNKFGEP
                     VIQGYTRSFGMKLPNGERREWIKPIMFTGGVGQMDAAHAVKGEPEKGMLVVKVGGPAY
                     RIGIGGGAASSMIQGENVEELDFNAVQRGDAEMEQKMNRVIRACIEMEGENPIISIHD
                     QGAGGNCNVIKELIYPAGAKIDIRKIPLGDHTLSVLEIWGGEYQEANALLIRSEKAEM
                     FGQLCNREKVPYAIVGVITGTGHIVLYDENDGNTPVNLDLKKVLGDMPQKIFSFSRIK
                     PILEPFRPEVPVTDALQRVLRLVSVGSKRFLTNKVDRSVTGLIARQQCAGPLQLTVSD
                     VAVISQSHFGTTGAALSIGEQPIKGLINPAAMARMSVAEALTNILWAKVSSIGDIRCS
                     ANWMWPAKLPGEGSALYDAAIAMKDLMVELGIAIDGGKDSLSMAAVVQSGDTRAVVKA
                     PGSLVISAYAPCPDITKVITPDIKRPGKTKLLYVDLGKGKYRLGGSALAHVYGQIGHE
                     SPDIDDAVMLKRAFEATQSLIEKDLILSGHDISDGGLIVALLEMAFSGNCGFHVNIKR
                     GTEEQKDVGSESLTPGTSGLQYVQTSALSALFAEEAGLVMEYLPEDGKKIFTILDDMN
                     IPYLTIGETLTERKITVEVNSQSVLSKGMEDLRGLWEETSYHLERLQANPQCVDEEKQ
                     NSYQRTGPHFHLTFSPVPTPSEILRRRTKPKVAIIREEGSNGDREMTSAFYHAGFETW
                     DVSMADLVHGDIPLDQFKGLAFVGGFSYADVLDSAKGWAGVIRFHKGIYEQFQRFYHR
                     EDTFSLGVCNGCQLMALLGWVPRQGIEDRFQPRFIRNRSGRFESRFSTVKILPSPSIL
                     LRGMEGSILGIWVAHGEGMAYFPHDGIKKDIIEKGLAPVRYVDDEGNITASYPFNPNG
                     STDGIAALCSPEGRHLAIMPHPERAFLKWHWPWMPEKWNEELVASPWLRMFQNAREWC
                     EGKVKNKSRE"
     gene            7919..8533
                     /locus_tag="VMU21_03230"
     CDS             7919..8533
                     /locus_tag="VMU21_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76567.1"
                     /translation="MWLKTLIRNVNVLNLVLMAAIILCALYILSPLLYPQAAFTLPSP
                     KKPLESKEEKGTENPPASPMEYTIIAEKNVLNPERKIPVDKPEAPPLPKPEFVLYGTL
                     IAGDDSLAYLEDQKASAAGKGKGRKPRPLKKGETMTGFTVKEIETDRVVMVRGEEKIE
                     VRVSGHTKQEKAAQATAPTTSVTPPQQQTSRPRGSRVRPAPPAQ"
     gene            complement(8640..9836)
                     /locus_tag="VMU21_03235"
     CDS             complement(8640..9836)
                     /locus_tag="VMU21_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO76568.1"
                     /translation="MNGLGFSLKTEGLIKEYHIYDRPSERLKELFLKTRKHRVFRALG
                     PLDIEVRKGETLGIIGENGAGKSTFLKLAAGVIEPSSGMIKVDGKVSSILELGTGFNP
                     EFTGRENVLLNGALLGLSSGEVSNRMETITGFADIGEFFDMPVKMYSSGMYLRLAFSL
                     AVHVDADIVLIDEALAVGDGAFEKKCIDRIWDLKKNGITMLYCSHSLYTVANFCDRAV
                     WLKAGSVQGIGGTKEVISRYEDYLREKELVEKTERPVAASIDGEKKMARVENLRLFAD
                     GYQVETSVQHSSDIEVVVDFEIFEDVKVHVGFAIDRNDGLCCYADSTQRQGLTPLHGP
                     GRGSLSILFRKLPLLGGAYKFVIFLLDETGICLFDRKESTILKVKTAEKEWGVCYMDH
                     EWKSHL"
     gene            complement(9836..10630)
                     /locus_tag="VMU21_03240"
     CDS             complement(9836..10630)
                     /locus_tag="VMU21_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO76569.1"
                     /translation="MKALLRNVLNGRHILKTMVIKDIKSRYVGSLFGPLWIIVTPLYQ
                     ILLYTLLFSTILRVRFDEGAGTSSFVVYLLAGMIPWLFFSEATMRGISAFIDNAHIIK
                     KVKFPMEVCVVTVIVSSAVTFLIYMFFYFIMLTLMGVLKLQTVLLFILPVAIQVLLTL
                     GLSFGLGSIAVFFRDITTMTGMVLNLVFFLTPIVYPANAIPEKLRGLFNINPFYFIVE
                     MSRSVLVRGKMPDAVSILYPSVFALVIFLIGYFIFNKTKEAFKDIL"
     gene            10918..11283
                     /locus_tag="VMU21_03245"
     CDS             10918..11283
                     /locus_tag="VMU21_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76570.1"
                     /translation="MNKVVPVIIAIALVLSFTGMTSSADKGEKITMVAGAVKAIDTKQ
                     KTITLETNELGKFTCILDDKTLLLGRKTIADVKVDDVAAVIYEDVNGKHIAKSITVAS
                     PAGSSSKEKTAPATSQEKK"
     gene            complement(11871..14453)
                     /locus_tag="VMU21_03250"
     CDS             complement(11871..14453)
                     /locus_tag="VMU21_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76571.1"
                     /translation="MNGKGYMNRQKEKVMPKVAKRSYFRPLVLLSILVLLTFLAYLRS
                     IHAPLIFDDFIYIRPLKLKGILTHFSLHVRSIAVFSFGLNYYLLGMNLVAFRITNIIF
                     HILSTFFAAYFTYTTLILPSMRDRYERFAEGKTPLYIALLVATLFLLHPIQTSAVNYI
                     TQRMAIMASMFSFAGFIFYVKGAAKRGRMAVLYYVLSSLSFILAIFSKENAVMVLFML
                     PVYDFFFLSTFQWKEFRKRFITVSVLLLSLALIVAYKMGAVGFIGKIFTLLSNPNQPM
                     ERLGYSGMGINWTPGEYLLTELRIVSRYIFLILVPAPSFMVFDYSNAYPVSKDLLNPL
                     TTLFSLVFLISALFFSLKYSKRAPLISFGILWYLTTISLESFIALGLDPYFEHRNYLP
                     SFGLFLGLATLLPYEDRLKLRVKKETIIILVALPLFALTFIRNGVWREGRLLWEEAVE
                     KAPNNVRARVNLGAAYVDQGLIDKAFEQYRTVLKMQPDNPNAHLNLGEVFFKKGLVDK
                     AIEEYEKALGSPQQVQARHKSDPLLERTNSDVHYNLGTAFQKKGLTDKAIEQYQTALR
                     IRSDFAEPYINLGIIYGELGTMDKAMEYFQAAVQLDPDFAKAHYNLGTALHKKRLVEA
                     AVEQYRIALKLDPGYAEAHSSLGSAYRDLGWIDKAIEHFQTAIKLRPDFANAHANLGA
                     VFFEKGLIDKAIEEDETALKLQPDLVETYKELGNAFYKKGLTDKAIEQYQIALKLRPD
                     LIEARKKLGNAFYKKGLIDKAIEQYQIALKLQPGSGEVHYNLGVAFYDKKLMNKAIEQ
                     YQIALKLKPGFAQAHNNLGSAYVELGSIDKAIEHFQTAEKLDPSNPVFRDNLARANAL
                     RNQK"
     gene            14731..15840
                     /locus_tag="VMU21_03255"
     CDS             14731..15840
                     /locus_tag="VMU21_03255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO76572.1"
                     /translation="MEERLLRELSRKTFCPDYSSNTIVSESPSDIGIKIILERSHTRF
                     VAISLNDTVYYSENWLEPLRGALEREFELVSPVCGDVFEVDMPYFSHLTFNDVAALMM
                     KKHARSYVRNSKIPPLAYLLRTESLRKLPLETKLDEIPALLKSALVPSSLVHRFGDYF
                     AFRRDDILPFVPHGVEKVLDVGCAKGLTGELIKKERGCKVFGVEINRKAAQIAMEKLD
                     EVFCVDIEKSDLPFNRDLDVIIFADIIEHLFDPWRVLENARKWLKPDGILIASIPNTG
                     HYSVILDLLRGRWDYVPFGLLCISHIRFFTRASIENMFTRSGYFIEEMSPQDFPLHVK
                     KQISTVLKKYIRVESISEDIFHHGYYIVAKQVKPL"
     gene            15840..16874
                     /locus_tag="VMU21_03260"
     CDS             15840..16874
                     /locus_tag="VMU21_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76573.1"
                     /translation="MSSPLRITYLLESTELWGGTKVALEQAELLSDAGYKVVVLSKDT
                     GPTWYPFKLSVIQVPHFDASTIPESNIVIGTYWPTVKAAVEAGKGIAVHLCQGYEGGF
                     KELYPFKAAIDEVYSYRIPKLTVSPHMNKFLIERFNAETYYVGQMLNRDIFHPVKVST
                     LKEHLDILNVLVIGPFDADVKNIRTALNGISMARLSLSIPLKLIRVSQFALSDDEKKI
                     IKPYVYHFHVPHLVMGEIYRKADLLVSMSKEEEGFGLPPLEAMGCGIPTILSRIPSHL
                     SYDDPQDYAVFVESTPGAVSKAILAVSESGELRERLRRRGLSVAERFTKESVLLRLFG
                     AFSKIVPYRI"
     gene            complement(17246..18193)
                     /locus_tag="VMU21_03265"
     CDS             complement(17246..18193)
                     /locus_tag="VMU21_03265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO76574.1"
                     /translation="MEIREVRKEDEEGIRSLFAACFGKELSREEWVWKYRGSPWGATA
                     SVAVEGGEIVAHYGGIRMEFFFRGRTFHVYQPCDVMTHPRYRARIFSRKGAMVRAAEH
                     FYATNPMDFAFGFASERHSILGTKQLGYTRHNHVRVLTKKVTSSGLLWRPLLKVKRGW
                     DSIEGGDLDSLWEKVRDSHGLSIMKSSRYLFWRYRDRPAREYFPLTIRSLCRTTPVAF
                     AVISLGGSDMFVLDFLCTETLRADTLLKVFGRIAIEHGRENLVLWAHRKDPLFGTLTS
                     NRFVQKEGIPNSFKVITDAIDSSFLVGNYWYRMGDYDAS"
     gene            complement(18285..19358)
                     /locus_tag="VMU21_03270"
     CDS             complement(18285..19358)
                     /locus_tag="VMU21_03270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO76575.1"
                     /translation="MISVVIVNYYSARQTERAIKSVLQSGVEREIIVVDNTCTTGERE
                     VLDEMRRSYGFSLVCNDKNLGFARACNQAYARSKGKYIFLLNPDAFVVPSCLGILREF
                     LENTPPAGSASPQVYWDDEMRYFFPHYTLPSPSMDLCVRLSSLSKTFRTIYSLSERRR
                     NLELWKSSTPVRVKNLHGGVVMVRRSAAEQSGGLFDERFFLFYEDTDLFFRLRKRGYS
                     LYTIPEAKAVHNYSHSEKKLEMMSRTTRLYFEKHFSRNLLLRISSFLPEGSLEGPYHD
                     YGIWNDPPLFPVPKAFRDGYLFEWSPSPLFIPSIGCFGKGGEFAFSRQVWDLLDDGRY
                     FNRFSPPSKRIGKYTTGYLEKRV"
     gene            complement(19605..20723)
                     /locus_tag="VMU21_03275"
     CDS             complement(19605..20723)
                     /locus_tag="VMU21_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76576.1"
                     /translation="MREERDRMMSVDTARFLNVFMDQNNKCNLRCIMCGFSDPRARKI
                     RKYDLPFWLFEKIAREVFPSAKYLALSCLTEPLMTRDFHQRLDLLKTYTVPFTEIVTN
                     GTLLTESIMIKMVDARVSRVAISLDGATPAIYESIRVGAKFDRVINNIKMFNAIKKEK
                     KSDLPSLRIDHVISRVNMNEFHSFLDLTESLEARAIDVRTISPFRNVKYREEEDESFF
                     RRMTAIKEELRQWTQKTGVEDVGYLRCSAEEIQLKDESGRNMTCRRPWDTVAIHANGD
                     VFPCMTWSRQGVGNISETSFEAVWNGSAFEAIRKEFEERKPGIDCLFCTIRKKDAESE
                     DDCFFTMLNKRPPKRSMRESLAELIRGIRRGHRKANKV"
     gene            complement(20726..23374)
                     /locus_tag="VMU21_03280"
     CDS             complement(20726..23374)
                     /locus_tag="VMU21_03280"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HUO76577.1"
                     /translation="MPFSFDTGGDAWVNEPNPYIIKKVLHESSFPAWNPLEGLGMPLV
                     GNLNTEVFNPLKVFLNLLPNPFSQDMFFLLRLLVMGIFTYLFLREMELSPLSCLLGSS
                     SFMLSGYSVWWINLHPLSTVTYLPAVFYFYEKWRNQEHVKNAFFMSLFLSFAFIAGKI
                     PDVIMGISLLFIYATWKGFTNNSVKGLCREGCKAVIATLSGILMAAVALTPFVELYSN
                     ASPLAKAIRTGAAGHTIPMISSVSLVQPLFLGWKNYSYGSWLKWTPDAILPHAGIVTL
                     LLVFCAAFNRRIVIRTLPYFVFSIGLFFVVYGILPSQVVSRLPVIRSIEFLKYNGMFY
                     FSLTIISAYAFDDLLSGEGSRKRFYLSLVAASLSILVYFIFLYRASPARMNGYMISLL
                     LLLSLFGLLIIGLAFHFFKSKQLFGIAVFLFLVGELFLYMPKDHPDRGDPYKEPPYLK
                     VIKDKEPYRISGDGSSLPPLVSGAMGLYDARAISVLMPGDYYSFFETLLGFSLPGTNN
                     PNPLFTATSPFSDLVGMKYVMSREPLERSKIEAEVTSHIGALRLVRLFGTMISHAIEG
                     GVTYGPFEAGGEKKFSLFFPLKFAFETRLRASEPFVFTGFALKDVPEGAEAKVRITIE
                     NRVAELNIKDGAWKDQWFDVSAYSGKVITIKIEGIGGGDGKMALGDFGLSPGNERERI
                     IYDNLLTLHKKEFDFLKYRGAYEGIHIYENTNVMDRAFVLHRVKAVKNLNEAIRELQE
                     GADFRNIALVPTPILPLNRGRSEDGASIFAGNAMGEGDKVTIKKYAPTEIMLDVESKG
                     GLLVLSDLYYPGWKVRVDGKEAQIIKAFGLLRGVGIGGGKAEVLFYYRPVSLYVGSII
                     SVMTFAAWIAFFIISRRKDRLCRFVK"
     gene            complement(23578..25230)
                     /locus_tag="VMU21_03285"
     CDS             complement(23578..25230)
                     /locus_tag="VMU21_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HUO76578.1"
                     /translation="MKKQFLLVTIMMGILLGFGSMSWAGEASIFDSAENILIRVCTFK
                     VCSNDSTKTCAADTDCTAGGTCNGNPTQYSAAFDVRDSTGALMPVGTFAVNDISGIAP
                     VPVTIAHNPFRITDITDGQSNPVVLSIPDVSLSLDSNSGAVYFFSTNGSVDVRKYEII
                     NLANGECVLYSQPLVAAPSTLAQLCVQPTTGAAPTVTTTATGWQWTCAGTTMGSNIGK
                     DANCSATSAAVPGVCGSSTNNGTFTTLSSTSSGLCANSSSQVKGFTAAGTGWQWTCGG
                     TNGGADSSQCSASLQTYTVTASATGGSISPSGLISAYYNATKSFTIAAPSGYTATVGG
                     TCGGSPATGTGTFSYATSQITHDCSVSATFALTPVNGLCGSANGGTFAVLSSGSSGLC
                     QSGFSVINFTATGSTGPWHWNCQGLYGGNDSPQCNANLQTAPPDLAITSAVGPGVLSS
                     TQSPTMKVTIINHGAGAAGPFNVTVYLGSAWFNPLVGASAIGTVRINGLAPGQSTTVT
                     IPTSASIILHSTYVLTVVADSSHELGDPNTTDNMSEQFLYVL"
     gene            complement(25489..25938)
                     /locus_tag="VMU21_03290"
     CDS             complement(25489..25938)
                     /locus_tag="VMU21_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76579.1"
                     /translation="MMIYADRFAGLFTVKTFWITSLLIVEMLFFSANCATIPEQGDLK
                     ASLKESAEKYWKMRMDEKYEDTYKMEDKEGLPPFEAYLQKVKAMRRFAVAKYTITNES
                     VEGEKGTVTVYLEVMLSAPRPIPSGFGDAWIYRNGRWRHVLPQEVHK"
     gene            complement(25942..27201)
                     /locus_tag="VMU21_03295"
     CDS             complement(25942..27201)
                     /locus_tag="VMU21_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HUO76580.1"
                     /translation="MKRVLKCCVLLFSLLLFLSCASTSRETKDNKETFNKETLTFTDI
                     SGGLPSEGLWRQNIALADMSGGGFLDIVAPPPRKAEADKLHPFIFSWSPGKGKWTEGS
                     FRFPEISDYSYAGIAVGDINRDGAPDIVLANHTGRIILLLNNKQGAFVESAFPMKEDF
                     SSRTVEIADVNGDGWPDVIAFSEGPFHSRYKPQGILVGINKEGKGWEVKVVEGGTDVY
                     GDSMAVGDLKGNGLKDVVVAPETMIQMFKKSIWFGDGKSNFNSYEVNPFPEKVTIAVR
                     AGDADGDGKDVAVFRLSSADSKGTVKQSLAALKWTGEGFADISEGLEAIEYPYVFDFA
                     DLYGDGKKQLVVLSDSGIGLYRHGPGGWVNIGFQRLSPDDTRGTYSLRAGRNRDGSLL
                     IVYNQGTGYAELKKGIRAFKVTLSGGK"
     gene            complement(27291..>28538)
                     /locus_tag="VMU21_03300"
     CDS             complement(27291..>28538)
                     /locus_tag="VMU21_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76581.1"
                     /translation="LFGVGTAYAVYGVDDAVPGKTAVIPVICSADSGTVDTGWAIADV
                     LGIVDPADTTTLGFGLHAVFHEPKNSGVAGDHHFCPTWHDVETDHCLCIMAGIGCGPG
                     HVMYAAAPPAQTISGAKYWIGYVTYQQEQPAPACAGPGASIDSDELLADRLVTWVYLT
                     DLPNGFASGFNAPSMEGPAEIGTELEENGSRAVAASRIFPRYVVQNNGPDTHTWWMLL
                     FGRADLPTKATGVLVGGRFLSCDIYDEEEGISSVCVSIPNEFNVFDITAAPTGAGCPG
                     SGSLNLLSGLFSPFPAAPPVCGVVSGTPCPGGPHNGYANCQIIEKDTFVGSAAPVTLT
                     GTTDGNGTAGHIITLLPSSTGQYSFFGWAHQKADGSSTQFNWDVVHPIHRHYCSPDSG
                     GNTITGNSPTDNFESCTCTGTGC"
BASE COUNT         7709 a         7015 c         6734 g         7080 t
ORIGIN      
        1 gggccggtaa aataataccc cctgtataag cttcttatgc cattccgggg tggatgcctt
       61 acggaatttt tgcaaacggt gtcgttttca tccattttct ctggatactc tttcttattt
      121 ttggtacatg ctggggggta aagaacaggg cggtcaggat ttttcatatc tcgggtctcg
      181 tttttgctgt cgttattcag ctctttgact ggtactgccc tctcacgcac cttgaggcat
      241 ggctgaggtc aaaacagagc cttactaccg cccatccttc ctcattcatc atttattatg
      301 tgcaaaagat agtatatgtc gaactttccc gttctatggt cattgtcctg acttttttcc
      361 tctgtggatt cagcgcatgg ctgtatctcc aaaagagaaa aaagccaaat aatccccttt
      421 gaaacgctta gatacggggc catatttttg gagtgccgat catccggact tatgctaaaa
      481 tatgcatatt cttgatgaat ggaggacagt atgaaggtaa tagaaggtga gcttcaggca
      541 gagggattga agtttggcat tattgtcagc aggtttaacg atttcataac aagcaagctc
      601 cttgatggcg ccctggacgc gcttcgcaga catggtgcca gtgaaaaaga tatagaggtc
      661 gtgaaggtcc cgggctcatt tgagattccc atggttgcgc ataagctcgc agccaaaggc
      721 acataccatg ctatcatctg ccttggtacg gtcatacgcg gcgcaacacc gcatttcgag
      781 tacgtatcag cagaggtgtc caaaggcata gcgtctgctt ccatggaaac agggatacct
      841 atcgccttcg gggtaatcac ctcagatact attgaacaag cggtggaaag ggctgggacc
      901 aagagcggga acaagggatg ggacgcagcc attaccgcca tagagatggc ccaggtaatg
      961 aagaaattag catgaaacgc cggaagggaa gggagtatgc ttttcaattt ctctttcagt
     1021 tcgactttac aggcgcaagg ccggaaaaaa aagattttga cctgttctgg gctgacaaga
     1081 atggggatgc tgacctcaga agattcacag aggatatcgt aagcggcacc cttcataacc
     1141 ttccagaaat agatgctgca atacaaaagg cagccgagca ctgggtcctg cagaggatgg
     1201 ccgcagttga cagaaacgtt ctcaggtttg cagcgtatga actcctctac cgctctgata
     1261 ttccttctgc agtgacgata aatgaggcca tcgagattgc gaagagatat tccacgaatg
     1321 agtcggcgtc ctttattaac ggaatccttg acaccattgc aaaaggaata gaaggcaaaa
     1381 ggatgcgata atgcgctacg cagttatttc agatgtccat gcgaacctcg aggccctcga
     1441 ggcggtcctc cgggatatta acaaaagggg aatagacgaa ttcctgtttc ttggagatgc
     1501 ggtggggtac ggaccaaatc ccaatgaatg tgtggagttg ctgagaaaga actgcaaaat
     1561 tcttctcgcg ggaaatcatg attgggcagc cattggtctg actgacatac agtatttcaa
     1621 tgaatatgcg aaggcagcca tcctctggac aagggaggta ctcacccctg agtcacggaa
     1681 attcattgaa accctgccag ttaccaagaa tataaaaagg ggaaatatgc tccttgtgca
     1741 ctcgaccccg aaggatcccg aggcatggca ttatctcctc accttatggg acgcagaagt
     1801 caattttcac tattttaccc atcgggtatg catgatcggt cacagccatc tgccttttgt
     1861 gatagaacga gtgccttcag gggagatgat tacgtataaa tctgaggcac gcctcggaaa
     1921 aaatgagcgg tatattatta atgccgggag cgtaggccaa ccaagggaca gggacccaag
     1981 ggcatgttat gcgatattcg atgatgatac gctggagttc gtgaggcttg aatatgccat
     2041 cgagaagaca cagaaaaaga tgcgtgatgc cgggcttcct ctcacgttga tcgaaagact
     2101 ttcaaaggga acgtagaaat gccgtgacgc gttatggcaa atgaccgctg aagcgttcca
     2161 gggggatggt cgtccaactg cccttaaggc tctagcctcc ggacgcacag gctctctgtt
     2221 gtttttatgc cgaatcaccg cgtgacgatc acgttattac tatagtaagg agacaccatg
     2281 ataccaaggt atacgcgacc ggagatgggc aggatatgga cacttgagaa cagatacatg
     2341 aagtggctcg aggtggagat cgctgcatgt gaggcatggg catccgtggg ggagattcca
     2401 aaatccgcac tgcagaccat aaggaaaaag gcccgctttg atgtcgacag aatcgatgag
     2461 atagaaaaga ccgtgagaca tgatgtcatc gccttcctca cctccgtggc ggagaatgtg
     2521 ggtcatgaat cgagattcat ccataaaggc ctcacctcgt cagacatcgt tgatactgca
     2581 cagtccctcc tcatgaggga agcctcggac atcatcataa aggatatcaa aggccttttg
     2641 agcgttatga aaaaacaggc cttcagatat aaggacacgc cctgcatggg ccgcagccat
     2701 ggtgtccacg cagagcccat gaccttcggc ctcaagttcg cgctctggta cgaggacatg
     2761 aggaggaacc tggaaagaat ggaggcggca agggagcgca tcagtatagg aaaactctcc
     2821 ggcgcagtgg gcaccttctc cagcatccct ccggtcattg aggagaaggt gtgtaaaaaa
     2881 ctcggtctca caccagaacc catcgcaaca caggtgattc aacgggatag acacgcagag
     2941 tacctcatga cccttgccct cattggtgca tccgtcgaaa ggatagcagt agagatacgc
     3001 cacctccaga ggacagaagt cctcgaggcc gaggagccct ttgaagcagg gcagaagggt
     3061 tcgtctgcca tgccgcacaa gagaaatcct gtgggatctg aaaacctgtc aggtctcgcg
     3121 agggttctca gggcaaatgc cctctcatca ctggagaaca ttgccctgtg gcatgaaagg
     3181 gacatatctc attcgtccgt ggagcgcatc acgattccag acagttctct gctcgctgat
     3241 tacatgcttg atcgcctcag gaccatactg gatggcctcc aggtgtaccc cgaaagaatg
     3301 aagcagaata ttgcggcaag ctacggactg tataactccc agcgggtaat gcttgccctc
     3361 actgagaagg ggctctcgag agaaagcgca tatgccattg tacagaggaa tgctatgaaa
     3421 agctggaagg agaagaagga gtttagaaaa ctgttgctcg gcgataaaga ggtgagaaga
     3481 taccttacgc ccaagatgat ggatgaactc ttttccctca gccattattt caagaatgtg
     3541 gattatattt tcggaagagt attcgggaga aggcgggcat gaaagcaagg cttttgctga
     3601 ccctggatac tgcatctgtc gaagaaaccc aaaggcttct ttcaactctc ggtgaggggc
     3661 tcattgccag tggttccatc tgcgattacc aattcgaaat agagacagac cagggtatcg
     3721 ttacagagaa gtgcatactc tcaggggata aggtcatcgc ctgaaaacga tgagaatgcc
     3781 agttggcata taagaaactc cagcatagat tgttgttata atactaataa acgcagttac
     3841 tgtatctttc caccggaggg tttaccatat tgccgttgat ctctttatac aggaaaccag
     3901 tcctttcaga aaccaagaag aatagcctcc tctccctcgt aagggaaaag gtctctcgcg
     3961 aggtaatgga tattcagaca gagtattgct tttatgtcga gagtcatgag ccgcttaccc
     4021 ctggtgaaaa ggatacctta caatggcttt tgagggaaac cttcgagccc cacaacctct
     4081 cctcagaatc ctttctggat aatggccaga ccatccttga ggtggggccg agaatgaatt
     4141 tcaccacagc ctggtcgacg aacgcggtct ctatatgtaa tggctgcggg ttgtcaaaag
     4201 tgtcccgcat tgaacgctcc aggagatacc aattggtcct ttatcggcag cggggagaag
     4261 aactcaagaa gggtttggag gggctcgttc atgatcgcat gactgaatgt ccctacaggg
     4321 agagattaac gtccttcgag tcagggattc atcctgagcc ggtatacgag gtgagaatcc
     4381 ttgaagaggg aaaaagtgcc atcgagaaga taaacaggga gctgggtctg ggccttgatg
     4441 aatgggacat caattactat tacttcctct ttgccaaaga tatgaagaga aatcccacca
     4501 atgttgaatg ctttgatctc agccaatcaa acagcgagca ctcaaggcat tggttcttta
     4561 agggaaagct cgtcatcgat gggagggcgg ttccccatac ccttatggag ataataaagg
     4621 aaccgtatag gaggaacccc aacaacagca tcattgcgtt cagcgacaac tccagcgcaa
     4681 ttagaggcta cagaataaga acaattcttc ccgagaagcc tggcaggcct tcccccttca
     4741 ccgaaaaaga gaaactctat cacctcatac ttactgcaga gacccataac tttcccagtg
     4801 gcgttgcgcc cttccctggt gcggaaacag ggacaggggg gagaatacgg gacgtacagg
     4861 ccgtgggaag aggagggctt gtggttgcgg gcactgctgc ttactgtgtg ggcaacctca
     4921 ggattccggg atataagctc ccctgggagg atgaatcctt tgcatacccc gaaaaccttg
     4981 cgactcccct ccagattgag atcgaggcga gcaacggggc atcagactac gggaacaaat
     5041 tcggagagcc cgtcatccag gggtacacga gatcctttgg gatgaagctg cccaatggag
     5101 agcgaaggga atggataaaa ccgatcatgt tcacgggcgg ggttggccaa atggacgcag
     5161 ctcacgcggt aaagggtgaa cccgagaagg gcatgcttgt cgtcaaggtg ggtggtcctg
     5221 cgtaccgcat aggaataggt ggcggagcgg catccagcat gatacaggga gagaatgtgg
     5281 aagagctcga tttcaatgcg gtgcagcgcg gtgacgcgga gatggaacag aagatgaaca
     5341 gggtcatccg tgcctgcatt gaaatggagg gtgaaaatcc catcattagc atccatgatc
     5401 agggagcagg agggaactgc aacgtgataa aggagcttat ctatcccgca ggagcgaaga
     5461 ttgacataag gaaaatcccc cttggagacc ataccctttc tgtactcgag atatggggtg
     5521 gcgagtacca ggaagccaac gcccttctca taagatccga aaaggctgag atgtttgggc
     5581 aattatgcaa cagggagaag gtgccttatg cgattgtggg tgtgattacg gggacagggc
     5641 acatagttct ttatgatgaa aacgatggaa acacaccggt gaaccttgac cttaagaagg
     5701 tcttaggaga tatgcctcag aaaatcttct ctttcagcag aatcaagccg atactcgaac
     5761 ccttcaggcc tgaagtaccg gtaacggatg ccctgcagcg ggtgctcaga ctcgtctcgg
     5821 tgggctccaa gagatttctc accaacaagg tggacaggtc agtgacaggc ctgatcgcac
     5881 gacagcagtg tgcaggacca ctccagctca ccgtctctga tgttgccgtg atcagccaga
     5941 gccatttcgg aacaacaggc gctgcgctct ccataggaga acagcccatt aaggggttga
     6001 taaaccccgc tgccatggcg aggatgtcag tggcagaggc cctcacgaac atactctggg
     6061 caaaggtgag cagcatcggg gatatacgtt gctcggcgaa ctggatgtgg cctgcaaaac
     6121 tccccggaga gggctcggca ctctatgacg ctgccattgc catgaaggat ttgatggtgg
     6181 agttggggat cgcgattgac ggaggtaagg acagcctttc catggcggca gtagtacaga
     6241 gtggtgatac gagggcggtc gtcaaggcac cgggctcatt ggtgatctct gcctatgccc
     6301 cttgccccga catcacaaag gtcattaccc ctgatatcaa aaggccgggc aagacgaaac
     6361 tgctgtatgt ggacctggga aagggaaagt accggctcgg gggctccgcc ctcgcccatg
     6421 tgtatggaca gatcgggcac gaatctccgg atattgacga tgccgtgatg ctcaaacggg
     6481 cctttgaggc aacccaaagt ctcattgaaa aagaccttat cctttcaggc catgacataa
     6541 gtgacggggg gcttattgtg gcgctgcttg aaatggcgtt ttccggaaac tgcggcttcc
     6601 atgtaaacat caagagggga acagaggaac agaaggatgt gggctcagaa tccctgacac
     6661 ctgggacttc cgggcttcag tacgtacaga cttctgcact ctctgctctg tttgccgaag
     6721 aggcgggact cgtgatggag tacctgccgg aggatgggaa aaagatattc acaattctgg
     6781 acgatatgaa tattccttat ctaacgatag gggagaccct gacggaaaga aagattaccg
     6841 tggaggtaaa tagtcagagc gttctcagta aaggcatgga ggacctaagg ggtttgtggg
     6901 aggagacgag ttatcatctg gagaggcttc aggcaaatcc ccagtgtgtt gatgaggaga
     6961 agcaaaatag ttatcaaagg acaggacctc atttccatct caccttcagc cctgtcccca
     7021 ctccgtcaga aatcctccgc agaaggacta agccgaaggt tgcaatcatt cgggaagagg
     7081 ggagcaacgg ggacagggag atgacctccg ccttttacca tgcgggtttc gaaacatggg
     7141 atgtttccat ggcagacctc gtacatggcg acattcccct tgaccagttc aagggtcttg
     7201 cctttgtggg cgggttcagt tatgcagacg tgctggactc agcaaaggga tgggcagggg
     7261 tcatacggtt tcataaaggg atatatgaac aattccagag gttttatcac agagaagaca
     7321 ccttcagcct cggcgtatgc aacggttgcc agctcatggc cctccttggt tgggttcccc
     7381 ggcaggggat agaagacaga tttcagccaa gattcatccg gaacagatca ggtcgttttg
     7441 agtccaggtt ttccacagta aagattcttc ccagcccttc gatactgctc agggggatgg
     7501 agggatcaat actcggcatc tgggttgccc acggagaggg gatggcttac ttcccccacg
     7561 acgggataaa gaaggatatc atcgaaaaag gtcttgcgcc ggtaagatac gtggatgatg
     7621 agggaaatat aaccgcgtct taccccttca atcccaacgg ctctactgac ggcattgctg
     7681 ccctatgctc ccctgagggc agacatctgg ccatcatgcc ccatcccgag agggcattcc
     7741 tgaaatggca ttggccatgg atgccggaga aatggaatga ggaacttgtt gcctcaccct
     7801 ggctcaggat gtttcagaat gcaagggaat ggtgtgaagg aaaggttaag aacaaaagca
     7861 gagaatagac acacccccct catctgaggt aatattaaca agcataggga aaaataaaat
     7921 gtggcttaag acgcttataa ggaatgtaaa tgtgctgaat ctagtgctga tggcagcgat
     7981 aatattgtgc gcgctctaca tcctctcccc gttgctctat ccacaggccg cattcacctt
     8041 gccctcacca aaaaaaccgc tggagagcaa agaagaaaaa ggcactgaga acccccctgc
     8101 atcgcctatg gaatatacca taatcgcaga gaaaaacgtc ctgaatccgg aaagaaagat
     8161 tcccgtggac aaacctgagg caccacccct tccaaaaccc gagtttgtgc tctacgggac
     8221 gttaatcgcc ggtgatgaca gcctcgcata tctcgaagac cagaaggcat ccgcggccgg
     8281 caagggtaag gggagaaaac cgaggccatt gaaaaagggt gagaccatga ccggctttac
     8341 cgtcaaagaa atagagacgg acagggtggt gatggtgaga ggcgaggaga aaattgaggt
     8401 gagggtatcg gggcatacca aacaggaaaa ggcggcgcag gccaccgcac ctacgacttc
     8461 tgtaacacct cctcagcagc agacctcaag accgaggggc agcagagtga gaccagctcc
     8521 ccctgcgcag taatggttta ttaccttttt aaaagaacct caatttttct ttattagata
     8581 ctaaagctgg gggttcttcc ttggcagcaa aatgcagact caacttcttc ccgattctat
     8641 cacagatgac tcttccattc atgatccata taacaaaccc cccactcctt ttcggccgtc
     8701 ttgaccttca gaatagtcga ttcttttctg tcgaaaagac atataccggt ttcgtccagc
     8761 aggaagatga caaacttata cgcaccaccc aagagaggga gttttctaaa aaggatgctc
     8821 agacttccac ggcccggccc atggagagga gtgagtcctt gcctctgcgt actgtctgcg
     8881 taacagcaga ggccatcgtt cctgtctatc gcaaagccga cgtgaacctt gacatcttcg
     8941 aagatttcaa aatccacaac gacctcgata tcggatgaat gctgaactga agtctccact
     9001 tgatatccgt cagcgaacag tctgagattt tccaccctcg ccattttttt ctctccgtca
     9061 atgctcgcgg caaccggcct ctccgttttt tcgacaagct ccttctccct taaatagtcc
     9121 tcgtatcttg agatgacctc ttttgtcccg ccgattccct gcacgctgcc tgccttgagc
     9181 caaaccgctc tgtcgcaaaa gttcgccacc gtataaagag agtgagaaca atagagcatt
     9241 gtaattccat ttttctttaa atcccatatc ctgtcaatac actttttttc aaaagcaccg
     9301 tcgccgacgg caagagcttc gtctattaac acaatgtccg catcgacgtg aacggcaaga
     9361 gaaaacgcaa gcctcaaata cattcccgat gagtacatct ttaccggcat atcaaaaaac
     9421 tccccgatat ccgcgaatcc tgttattgtt tccatcctgt tgcttacctc gcctgatgaa
     9481 agaccaagga gcgcgccatt gagaagcaca ttttctcttc cggtaaattc gggattaaat
     9541 cctgtcccga gttccagtat cgatgagacc ttaccgtcca ccttgatcat ccctgacgac
     9601 ggctcgataa cgcctgctgc gagtttgagg aaggtactct tgccggcacc gttttctcct
     9661 atgataccca gcgtttctcc cttgcgcacc tcgatgtcca aagggccgag ggccctaaaa
     9721 accctgtgtt ttctcgtctt aaggaagagt tccttcagtc tctctgaagg gcggtcgtaa
     9781 atatgatatt cctttataag cccttctgtc ttcaatgaaa atccgagccc gttcatcaaa
     9841 ggatatcctt aaacgcttct tttgttttat taaagataaa atatccaata aggaatatca
     9901 ccagcgcgaa gacagatggg taaaggatcg aaactgcgtc aggcattttc cccctcacaa
     9961 gcacacttcg ggacatctcg actatgaaat aaaatgggtt gatattgaat agcccccgta
    10021 acttttcggg aatggcattt gcagggtaaa caatgggcgt taaaaaaaat acgaggttca
    10081 aaaccatacc ggtcatcgta gttatatctc tgaaaaaaac tgcgatgctg cccaggccga
    10141 aggaaagccc caatgtcaac agtacctgaa tggcaacagg caatataaaa agcaaaactg
    10201 tctggagttt caaaacgccc atcaaggtca gcatgataaa ataaaaaaac atgtatatca
    10261 gaaaagtgac cgcagatgag acaatcaccg tcacaacaca aacttccatt ggaaatttca
    10321 ccttctttat tatatgcgca ttatcgataa aggcggagat tcctctcatc gtcgcttcag
    10381 aaaagaaaag ccatgggatc attccggcaa gaaggtaaac cacaaaagat gaggtcccgg
    10441 cgccctcgtc gaaacggact ctcaatatgg tagaaaaaag cagtgtatac aggaggatct
    10501 gataaagagg ggtgactatt atccagagcg gaccgaagag agaacccacg taccttgact
    10561 ttatatcctt aatgaccatg gtcttaagga tatgccgccc gttcaggaca tttcgcagta
    10621 aggctttcat ctaatgactc tcatcctaaa ttgttattga cagagtgttg accactagtg
    10681 ttctatttag ctattgaact tttatatcgg agaacattaa ggaatatatt aacacattac
    10741 caatgggcaa gataacatcc gtttcatgca agaggatttt tgctgtctct ctccaaagtt
    10801 gcgtcatccg ggatctttgc taatactctc acgtatccgg ttgaatactt tgctccattt
    10861 ttgttaaaat gccacatatc taaaagttac aacctttttt gaaaggaggt gatctcagtg
    10921 aataaagtag tccctgtcat tatcgcaatc gctcttgttt tgtcttttac aggaatgacc
    10981 tcgtcagctg acaaaggaga gaagataact atggttgcag gtgcagtgaa ggcgattgac
    11041 acaaaacaaa aaactatcac gctagagacc aatgaattgg gtaaattcac atgtattctt
    11101 gatgataaga ctctactatt gggtagaaag accattgctg atgtaaaggt cgacgatgta
    11161 gctgctgtca tatatgagga cgtgaatggc aagcatatag cgaaaagtat tactgtagca
    11221 tcccctgcag gatcttcttc aaaagaaaaa actgcgccgg caacgtccca agaaaagaag
    11281 tagctggaac cggggagagc gccaaggaca tgcatcctcc ttctcgtttc taaagcgcac
    11341 tgttactccg tgattggccg catgaatctg gttgatgaga cgtctgagtc gccgcaggtc
    11401 gtcctaatac tccctgcaag ctgcggagaa ctcgttgcgc ggtcctgata acgccttgac
    11461 ttcttgaaag gatactaact gcaggcattt ggggaaaacg gaaaattgat gagctggttg
    11521 tgaaatagct cttaatcagc gggtcggagg ttcgaatccc ccactgctcg ccaataaaaa
    11581 atgaggactt acgagatcat ctctccgggt cctttttgtt ttggctacac ttttggcgaa
    11641 cgcgaaaggc aggactcatc cctatccctc gatgggatgt atccctgtta ttataatccg
    11701 ggttaccttc tccttatgcc aaaccttaat aatgagccgg gcgtcaggcg tcctttctat
    11761 cttacagagt ttcgactgtc gttttattaa gactggacag caacaaagcg aggcgtcgct
    11821 ttcttctctt ctcccctctc agttcatcct gcttttttgc attgttgatg ttatttctga
    11881 tttcttaatg catttgctct cgccaaatta tcccggaaaa ctggattgga cggatctaat
    11941 ttctcggctg tctgaaaatg ctcaatggct ttgtctatcg aacccaactc aacataggcg
    12001 ctcccaagat tattatgggc ttgagcaaaa cctggtttta atttcagggc aatttgatac
    12061 tgttcgatgg ccttattcat caacttcttg tcatagaaag cgacccccaa attataatga
    12121 acctcacctg aaccgggttg taattttaaa gctatttggt attgctcaat cgccttgtct
    12181 atcaaccctt tcttataaaa ggcgttccct agttttttgc gcgcttctat gagatcaggt
    12241 cgtaatttta aagctatttg gtattgctca attgccttat ccgtcaaccc tttcttataa
    12301 aaggcattcc ccagttcctt gtatgtttct acaagatcag gctgtaattt taaagcggtc
    12361 tcgtcctctt caattgcctt atctattaac cctttctcaa aaaaaacagc ccctaggtta
    12421 gcatgggcat tagcaaaatc gggccttaac tttatagcgg tctgaaaatg ttctatcgct
    12481 ttatcgatcc agcccagatc cctgtaagca gatcccagac tgctatgggc ctcagcatat
    12541 ccgggatcta actttaaggc aatccgatat tgttcaacgg cagcttccac caatcttttc
    12601 ttatgcaagg cggttcccag attataatgt gctttcgcaa aatcgggatc tagttgcaca
    12661 gcggcctgga aatattccat cgctttatcc atagtaccca gttccccata aataattcca
    12721 agatttatat agggttctgc aaaatcggat cttattctca acgcagtctg atactgctca
    12781 atagccttat ccgtcaagcc tttcttttga aaagccgttc ccaggttata atggacatcg
    12841 gaattagttc tctccagtaa agggtccgat ttatgacggg cctgaacctg ctggggcgag
    12901 cctagggcct tttcgtactc ctcaatcgcc ttatctacca ggcccttctt gaaaaaaacc
    12961 tctcccaggt tgagatgggc gttcgggtta tccggttgca tttttagaac ggtccggtac
    13021 tgttcaaacg ctttatctat taggccctga tccacatatg ctgctccaag attaacacgg
    13081 gccctcacat tgtttggggc tttttcaacg gcttcttccc aaagaagtct tccttctctc
    13141 catacgccgt tccttatgaa agtcagggcg aacagaggaa gagcaacaag tatgataatc
    13201 gtctctttct taacccttaa cttcaatctg tcctcatacg gcagaagagt ggccaaccca
    13261 agaaaaagtc cgaaacttgg cagataattt ctgtgctcaa agtacgggtc cagcccaagg
    13321 gctatgaaac tctcaagaga gatcgttgta agataccata atatcccaaa agagattagg
    13381 ggagcccttt tgctatattt tagagaaaag aacaaagcgg atatcaagaa aacaagggag
    13441 aataatgtgg tgagaggatt gagcaggtcc tttgatacgg ggtaggcgtt ggaatagtcg
    13501 aataccataa atgaaggcgc cggaacaagt atgagaaaga tgtatcttga aactatccgc
    13561 aattccgtca acaggtattc gccgggtgtc cagttgatgc ccatccctga atagccaagt
    13621 ctctccatgg gctgattggg gtttgagaga agggtaaaga tctttccgat gaagccgact
    13681 gcacccatct tgtaggccac tattaatgcg agggacagca ggagcacgga gaccgtgata
    13741 aacctcttgc ggaattcttt ccactggaac gtcgaaagaa agaagaagtc atagaccgga
    13801 agcatgaaga ggaccatcac tgcgttctcc ttcgaaaaga tagccaggat aaaagagaga
    13861 gacgacaaga cataataaag caccgccatt ctccctctct tagcagcacc ctttacataa
    13921 aaaatgaatc ccgcgaatga aaacatgctc gccattatcg ccattctttg agtgatataa
    13981 ttcaccgccg aagtctgtat agggtggagc aggaaaagtg ttgccactaa cagtgcgata
    14041 tacaaaggtg ttttaccctc tgcaaacctt tcatatctat ctctcatgga gggaaggatt
    14101 agcgtagtat atgtaaaata agcagcgaag aaagtggaaa ggatatgaaa gattatattc
    14161 gtaatcctga atgcaacaag attcatgccc aaaagatagt aattcagtcc aaaagagaat
    14221 acggcaatag aacggacatg taaggagaag tgggttaaaa tgcccttcag ttttaagggc
    14281 ctgatatata tgaaatcgtc aaagattaat ggggcatgaa tggacctgag gtaagcgaga
    14341 aaggtcagga gcaccaagat agagagaaga acgagaggtc gaaaataact tctcttagcg
    14401 actttcggca tcactttttc cttttgccta ttcatgtatc cttttccgtt catacatcat
    14461 aacattctca attcttattt ctttgatcaa tcacattatg aggatatttt agaggttcgt
    14521 taacataaca acatattgaa catctgtcaa ttccccagca cttcctgagg caaacaatca
    14581 cataagctca ttccccttat attctatatg tttatgttag aataatgtta gtaaggataa
    14641 ggacgcagat atcgaataaa tataatgata aaacccctta gggaagaagt cacctttatt
    14701 tatatagaaa ggcctctcgc cgagccctcc atggaggaga gacttctcag agagttatca
    14761 agaaaaacgt tctgtcccga ttactcctcc aataccattg tctcagaaag tcctagcgat
    14821 atcggcatta aaataattct ggaaaggtct cacacccggt ttgtcgctat ttctcttaat
    14881 gatacggttt attactcaga gaactggttg gaacctttaa gaggggcttt agagagagag
    14941 tttgagcttg tctcgcctgt ctgcggcgat gtctttgaag tggatatgcc gtatttttcc
    15001 cacctgacct ttaacgacgt ggcggccctg atgatgaaaa aacacgcgcg ctcctatgtg
    15061 cgcaattcca agatccctcc tctggcttac ctgctgcgca ctgaaagtct tcgcaagctc
    15121 ccgcttgaaa ctaagcttga cgaaatcccc gctcttttaa aaagcgccct tgtgccttca
    15181 tcccttgtgc acagattcgg cgattacttc gcgttccgaa gagatgatat cttacctttc
    15241 gtcccgcatg gtgtagaaaa ggtccttgat gtcggctgcg caaaagggct aacaggcgaa
    15301 ctaatcaaga aggagcgtgg gtgcaaggtt ttcggtgtag aaatcaacag aaaggcggcc
    15361 cagattgcta tggaaaagct cgacgaagtc ttctgtgttg atatagaaaa gtcagacctc
    15421 ccgtttaaca gagacctgga cgtcatcata tttgccgaca taatagaaca cctgtttgat
    15481 ccgtggcgcg ttctcgaaaa tgcccgaaaa tggctcaagc ccgatggaat tctaattgcc
    15541 tctattccga ataccggaca ttattcggta atcctggatc ttctcagagg aagatgggat
    15601 tacgtgccct tcggacttct ctgcatctcg cacattagat tctttaccag ggcatccata
    15661 gagaacatgt ttacaagaag tggatatttt atagaagaaa tgagtcctca ggattttccg
    15721 ttgcacgtca aaaaacagat atcgactgtg ctgaagaaat acataagagt ggagagcatt
    15781 agcgaggata ttttccacca cggctattac atcgtcgcca agcaggtgaa gccgctctga
    15841 tgtcctcgcc gctgaggata acgtatcttc ttgaaagtac cgaactgtgg ggcggcacca
    15901 aggttgccct tgaacaggcc gaactcctct cggacgcggg atataaggtc gtcgttctct
    15961 caaaggacac aggaccgact tggtatccct ttaaattaag tgtcatacag gtgcctcatt
    16021 tcgatgcttc tacaataccg gagtctaata ttgttatagg cacatactgg ccaacggtca
    16081 aggctgccgt tgaagcggga aaggggatcg cggttcatct ctgtcaggga tatgaagggg
    16141 gctttaaaga gttgtaccct ttcaaagccg ctatagatga agtctactca tatagaatcc
    16201 ctaagctcac ggtctcaccc catatgaata aattcctcat cgagagattc aatgctgaga
    16261 cctattacgt gggccagatg ttaaacaggg atatctttca ccccgtaaaa gtcagcacac
    16321 taaaagagca tctcgatatc ctgaatgtgc tggtcatagg tccctttgat gctgatgtga
    16381 agaatatccg gacggctctc aatgggatat cgatggccag actgagcctt tccataccgc
    16441 ttaaacttat aagggtctcg cagttcgccc tttcagatga tgagaaaaaa atcataaaac
    16501 cctatgtata tcatttccat gtgccgcacc ttgtcatggg tgaaatttac agaaaggcag
    16561 acctcctcgt ttccatgtca aaggaagaag agggatttgg attgccccct ctcgaggcca
    16621 tgggatgtgg gatacccacg atactgagca ggataccttc ccacctctcc tatgatgacc
    16681 cccaagatta tgcggtcttc gtggaatcca ctccgggagc agtttctaag gctattctgg
    16741 cagtatcgga aagcggtgaa ctcagagaga ggctccgtag gagaggcctt tcggtcgctg
    16801 aacgatttac gaaagagtct gtgctgttaa gactcttcgg tgctttcagt aaaatcgttc
    16861 cgtataggat ataagacaac aatttgatct ggctatagct gacacagcaa aacattgaac
    16921 tgaatccaag gcttgacaag atataagaga atgatggcgg ggaattaacc gtcattgcca
    16981 tcaatttcct gagttcctgc gccgaagagg ttacgcccga aacaagcgcg gaaaggttta
    17041 tgataaccag tgccgatatt tacttgtctc taatagagca atgcaatctc atcgcatttc
    17101 cttcccgaag atcaacactc tccgcacgtt cgtatcggat caaaagaggt cgctgtctcc
    17161 tataagtgta atagaaaaat atctctttat catctccact tcctatcagg aaatgtgcga
    17221 ccacacagca taatgatctc ttgtactaac ttgcatcata gtctcccatg cggtaccagt
    17281 aattcccaac gagaaacgag ctgtctatgg catcagttat caccttaaaa ctgttcggta
    17341 ttccctcttt ctgtacgaac ctattactcg taagggttcc aaaaagcggg tccttccgat
    17401 gagcccatag tacaagattc tcccgtccat gttcaattgc tatccttccg aacactttca
    17461 gaagagtatc tgctcgcaac gtctcagtgc aaaggaaatc cagaacaaac atatcagatc
    17521 ctcccaggga aatgaccgca aaggcgactg gcgtagtcct gcacaagctt ctaatggtga
    17581 gcgggaaata ctctcttgca ggcctgtccc ggtatcgcca gaaaagatac cggctgctct
    17641 tcatgatcga aaggccgtgg gagtccctga ccttttccca aagggagtcg agatctcctc
    17701 cctcgatcga gtcccatcct ctcttcacct tcagaagagg tctccacagg agaccggaag
    17761 aggtgacttt cttggtgagc actctgacat ggttatgtct tgtatatccc agctgctttg
    17821 ttccaaggat tgagtgcctc tcagaggcga atccaaaggc aaagtccatc ggattggtcg
    17881 cataaaaatg ctcggctgcc cttaccatcg ccccttttct cgagaaaatt cttgccctgt
    17941 accggggatg ggtcattacg tcacaaggct ggtagacatg aaaggttctg cccctgaaaa
    18001 agaattccat cctaatgccg ccataatgag caacaatctc ccctccttcc actgcgaccg
    18061 acgccgtggc gccccatggc gaacccctgt atttccagac ccattcctcg cgggagagtt
    18121 ccttaccgaa gcaggctgcg aaaagggaac gaataccctc ctcgtcctcc ttcctgactt
    18181 ctcttatttc catctattct cctcttttgg agaaaagatc tcgttctcgt acgcagagta
    18241 catcttatct tcctctcagg gaaggtcgtc ccgaatagtg aaattcatac ccttttctcc
    18301 aaatagccgg ttgtatattt cccaattctc tttgaaggag gcgaaaagcg attgaaatac
    18361 ctgccgtcat caagcagatc ccagacctgc cgtgaaaaag caaattctcc ccctttgccg
    18421 aagcatccga ttgaagggat aaataatggg ctggggctcc actcgaatag atacccgtcc
    18481 cggaatgctt tggggacagg aaataacggc gggtcattcc atatcccgta atcgtgatac
    18541 gggccttcaa gagaaccttc aggtaagaaa gatgaaatcc tcaaaagaag gtttctgctg
    18601 aagtgtttct caaagtaaag gcgggtcgtt cgagacatca tctccagctt cttctcgctg
    18661 tggctgtaat tgtggaccgc ctttgcttca ggaatagtgt aaaggctgta ccctcttttt
    18721 ctcagtcgga aaaaaagatc ggtatcttca tagaacaaga agaacctctc atcaaagagt
    18781 ccaccggatt gttccgccgc agaccttctg accatgacga ctccgccatg gagatttttt
    18841 accctaacag gcgttgaaga tttccagagt tcaaggttcc tccgtctttc ggagagcgaa
    18901 taaatggtcc tgaacgtctt agagagagac gacagccgca cgcaaagatc cattgacggg
    18961 gacggaagag tgtaatgggg gaaaaagtac cgcatttcgt catcccagta aacctggggg
    19021 gatgccgagc cggcaggcgg agtattctca aggaactctc tcaatatgcc aaggcaagag
    19081 ggcacaacaa atgcatccgg attcaaaaga aagatatatt ttcccttcga gcgggcatat
    19141 gcctgattgc acgctctcgc aaacccgaga ttcttgtcgt tgcagacaag gctgaatccg
    19201 tacgacctcc gcatctcatc gagaacttcc ctctcgccag ttgtgcaggt gttgtccaca
    19261 acgatgattt ctctttccac cccactctgc aaaacggact taatggccct ttctgtctgt
    19321 ctcgcagaat agtaatttac gatgacaacc gagatcatct tgggacctga ttaaaaggtt
    19381 cattcaaatg ctcagcgggt ttaagcggct tccggctaag caaatcgtgt tcttcgcttt
    19441 tcgaacatct ctccccataa tggcgaacag ggagcgccat ttgttgccgt ggctttctca
    19501 tcacgtattc cgaaaagctg tcggcatgtt cctgaaagat tgcctgagca tccattccct
    19561 gcaagattcc tgtatcgtcg ccatttttac tatctccgtg catactaaac tttattcgct
    19621 tttctatgcc ctcgacggat tcccctaatc aattcggcga gagattcacg catggaccgt
    19681 ttgggcgggc gtttgtttaa catagtgaag aaacaatcgt cttcgctttc agcgtctttt
    19741 tttcttattg tgcaaaataa acagtcgatc ccgggttttc gttcttcgaa ttccttccgt
    19801 attgcctcaa aggcggagcc attccataca gcttcaaaac tcgtttcgga tatattgccc
    19861 accccctgtc gggaccatgt catgcatggg aacacatctc cattagcatg gatagcaaca
    19921 gtatcccacg gacgccggca cgtcatattt ctgcctgatt cgtcttttaa ctgtatctcc
    19981 tctgcgctac acctcagata cccgacatct tccactcctg ttttctgagt ccactgccgc
    20041 aattcctcct taattgccgt cattcttcga aaaaatgatt cgtcttcctc ttcccgatac
    20101 ttgacattcc gaaacggcga gatcgtgcgc acatcaattg ccctggcttc aagggattct
    20161 gtcagatcca gaaatgagtg aaattcattc atgttcaccc ttgatattac gtggtcaata
    20221 cgaaggctgg gcaaatccga tttcttctct ttttttatcg cattaaacat cttgatatta
    20281 ttgatcaccc tatcgaattt agcgcccaca cgaatagatt catagattgc aggggtagcg
    20341 ccatccaatg atattgccac cctcgaaaca cgcgcatcaa ccatcttgat catgatcgac
    20401 tctgtaagca gtgtgccgtt cgtcactatt tcagtaaagg gaacagtata tgtcttcaac
    20461 aagtccagtc gctgatggaa atccctggtc atgagtggtt cggtgagaca tgaaagcgca
    20521 aggtacttgg cagaggggaa aacctccctc gcaatctttt caaaaagcca gaagggcagg
    20581 tcatacttgc gtatctttcg agccctggga tcactgaatc cgcacataat gcatctcaaa
    20641 ttacacttgt tgttttggtc cataaaaaca ttcagaaatc ttgccgtatc aaccgacatc
    20701 attctatctc gctcttccct cattcctatt tcacaaatcg gcagagcctg tcctttcttc
    20761 tcgaaattat gaaaaatgca atccacgcgg caaaggtcat aactgaaata atggagccga
    20821 cataaagact aacgggtcgg taatagaaca ggacttcagc cttccctcct ccaatcccta
    20881 ctcccctcaa aaggccaaat gcctttatta tctgggcctc cttcccatcg actctcacct
    20941 tccatcccgg ataataaaga tcgcttagga caagaagccc gcccttcgat tccacgtcca
    21001 acatgatctc agtgggggca tacttcttga ttgtgacttt atctccctca cccatggcat
    21061 ttcctgcaaa aatagatgcc ccgtcctcgc tcctgcccct atttaaaggg aggatcggtg
    21121 tgggtaccag cgcaatattc ctgaagtcag cgccttcctg aagctctctt attgcttcgt
    21181 tgagattctt caccgctttc accctatgca gaacaaaggc cctgtccata acattcgtat
    21241 tctcgtatat atggatacct tcatatgcgc ctctgtattt tagaaaatca aactcttttt
    21301 tgtgaagtgt caggaggttg tcatagataa tcctctctct ctcattcccc ggggaaagac
    21361 caaaatcccc gagcgccatt ttcccgtcac cgccgccgat gccttctatt ttgatggtga
    21421 tgaccttccc cgaatacgcg gagacatcaa accactggtc tttccaggcg ccatctttta
    21481 tgttcaattc agcaactctg ttttcaattg ttattcttac tttagcttct gcgccttcag
    21541 ggacatcttt cagcgcaaac cccgtgaaga caaaaggttc cgatgcccta agtctcgttt
    21601 caaaggcaaa cttcagaggg aaaaagagag aaaacttctt ctcgccgccc gcttcaaaag
    21661 gcccataggt cactcctccc tcaatagcat gacttatcat tgttccgaag agacgcacta
    21721 atctcaaggc gccgatatga gatgttacct cggcctcgat tttcgaccgc tccagaggtt
    21781 cccgactcat tacgtatttc atgccgacaa gatcgctgaa aggagacgtt gcggtgaaca
    21841 gaggattcgg gttgtttgtg cccggcagac tgaaccccaa aagggtctca aagaaactat
    21901 agtagtctcc cggcatcaag acgctgatgg cccttgcatc atacaacccc atagcgccgc
    21961 tcacaagagg aggcagagag cttccatcgc cggaaatccg gtaaggctct ttatctttga
    22021 tgaccttgag ataaggcggt tctttgtagg ggtctcctct gtccggatgg tctttgggca
    22081 tgtaaagaaa aagctcgccg acaagaaaca gaaagaccgc tatgccgaat aactgtttgc
    22141 tcttaaagaa atggaaggcc aaccctatga taagaagccc aaaaagagaa agaagaagaa
    22201 gaagggagat catatagccg ttcatgcgtg caggactcgc tctgtacagg aagatgaaat
    22261 acacaagtat tgatagcgag gccgccacga gggaaagata aaatctcttc ctgctccctt
    22321 cacccgacaa gaggtcatca aaggcatagg ccgagattat agttaaagag aaatagaaca
    22381 tcccgttata ctttaagaac tctatgcttc tgatgacagg caatctcgaa acaacctggg
    22441 atggcaagat accgtatacc acaaaaaaca aacctatcga gaatacgaaa taaggaaggg
    22501 tccttatcac aatcctgcga ttaaacgctg cgcaaaaaac aagcagcagt gtcactatgc
    22561 ctgcgtgggg caggatcgca tccggtgtcc acttgagcca tgagccgtaa gaataattct
    22621 tccagccgag aaaaaggggc tgaacaaggg atacggagct gatcataggg attgtatgac
    22681 cggcggcgcc cgttctgatg gccttcgcaa gtggagatgc attggaatag agttccacaa
    22741 aaggagtcag agcgacagct gccattagta tgccggagag agttgcgata accgccttgc
    22801 agccctcccg gcaaagtcct ttgacggaat tattcgtaaa gcccttccat gttgcataga
    22861 taaagagaag agagataccc ataatcacat caggtatttt ccctgcaata aaggcaaagg
    22921 aaagaaacaa cgacatgaaa aaggcgttct tcacgtgctc ctgatttctc catttttcat
    22981 agaaatagaa aactgccggc agatatgtca ccgtgctcag aggatggaga ttgatccacc
    23041 agactgagta tcccgaaagc ataaaagatg aagagccgag gaggcatgat aaaggggaca
    23101 gttccatttc ccttagaaaa agataggtaa atatgcccat gacaagcaat ctcaggagaa
    23161 agaacatgtc ctgtgaaaaa ggatttggca agaggttaag aaatactttt aagggattga
    23221 agacctctgt gtttagattc ccgacaagcg gcatcccgag gccttcaagg ggattccatg
    23281 ccggaaacga actttcatgc agaactttct tgattatata gggattcggc tcattgaccc
    23341 atgcatctcc tcctgtgtca aaggaaaagg gcattccggg cctccggcct gaaaaaccgt
    23401 aggggccgga cggagttgtg cctggaacga gagaagaggt ggaaagagtc ttgtcgaaaa
    23461 ggagaacgtc ataaaaaaaa ataacgagca agagaggcaa aagcaaccag agggatttgt
    23521 ttttcatatt tcttcatcca ggggggaggg ctgtcccccc ccggatgaaa gattacgcta
    23581 taaaacgtaa aggaactgtt ctgacatgtt atctgtagta ttggggtcgc cgagctcatg
    23641 agaggaatcg gcaacaacgg tgagcacata agtcgaatga agtattatcg acgctgatgt
    23701 cggaatcgta acggtcgtag attgcccggg agccaacccg ttgatacgca cggtgcctat
    23761 tgctgaggct cccacgagtg gattaaacca ggcagagccg agatagactg ttacgttaaa
    23821 cgggccggcc gctccagctc catgattaat aatagttact ttcatagtag gggattgagt
    23881 cgatgataag acaccgggtc ccacagcact cgttattgca agatcaggcg gcgccgtctg
    23941 gaggtttgcg ttacattgtg gcgaatcatt accgccgtat aacccctgac aattccagtg
    24001 ccacggccct gtgcttcctg tggctgtgaa gttgatcaca gaaaatccgg attgacataa
    24061 accgctgctg ccagacgaca agactgcaaa tgtccctccg ttggcggagc cgcagagtcc
    24121 gtttactggc gtcaatgcaa atgtcgccga tacagaacag tcatgcgtta tctgactcgt
    24181 cgcgtagctg aatgtcccgg tcccggttgc gggtgaaccg ccgcaggtgc ctcccactgt
    24241 cgccgtatat ccgctgggcg ccgctatcgt aaatgacttg gtagcgttat agtaggccga
    24301 aatcaggccc gacgggctta tgctcccgcc cgttgccgat gccgtcaccg tgtatgtctg
    24361 gagacttgcg ctacattgtg acgaatccgc cccgccgttt gttcctccac atgtccactg
    24421 ccatccggta ccggcagcag taaagccttt aacctggctg ctggagtttg cgcacagacc
    24481 agagctggtt gacgataagg ttgtaaaggt gccgttattc gtcgagcttc cgcagactcc
    24541 gggaacggct gctgacgttg ccgaacaatt ggcatccttt ccaatatttg accccatggt
    24601 tgttcccgcg caggtccact gccagcctgt cgcagttgtt gtcactgtcg gagcggcgcc
    24661 cgtcgtcggc tggacgcaga gctgtgcaag cgttgacggg gcagcgacaa ggggctgcga
    24721 ataaagaacg cactctccgt ttgcaagatt tattatttcg tatttcctga catccacgct
    24781 gccatttgta ctaaagaaat ataccgcgcc gctgttacta tctaaggata ggctcacatc
    24841 agggattgac aggacaacag gatttgattg gccatcagta atatcagtaa tccggaaagg
    24901 attgtgcgcg atagtaaccg gaaccggtgc gatccctgaa atatcgttca cggcaaacgt
    24961 tccgacgggc attaacgcgc cggtggaatc cctcacgtca aaggccgccg aatactgggt
    25021 gggatttccg ttgcaggtcc ctcctgctgt gcaatccgta tctgcagcgc aagtcttggt
    25081 ggagtcgtta gaacacactt tgaaagtgca aaccctgatg agaatattct ccgcactatc
    25141 aaaaatgctc gcctctcctg cccaggacat gctgccaaag cccagtagaa tacccatcat
    25201 gatggtcact aatagaaact gctttttcat tccctgcctc cttctatact ccaaaactat
    25261 gccgatctat gcgtcatggg agatgatgta gcggacccat gtcacataac gcctcttcct
    25321 gattatttta tgagcatatt ccatacctgc caataacctc ttctattaga gcacaaacag
    25381 ttcagcatgt caataatttt cttttattca caaagattat taacaaaact taatctaacg
    25441 ctttaggatg gtgtatgacg atatctgtca cattccgggc aacaaatgtc acttatgaac
    25501 ctcctgaggt agaacatggc gccatcttcc attcctgtag atccaagcat cgccaaaacc
    25561 cgagggaatg ggcctcggcg cagaaagcat gacttcaagg taaaccgtca ctgttccttt
    25621 ttcgccttcg acgctttcat tcgttatagt atacttcgct acagcaaacc tccgcatcgc
    25681 cttcaccttt tgcaggtaag cctcaaaggg gggcaagcct tccttatctt ccatcttata
    25741 ggtgtcttca tatttttcat ccattcgcat cttccagtac ttctccgcag actctttcaa
    25801 agacgccttt aggtccccct gttctgggat agtggcacaa tttgcagaaa aaaagagcat
    25861 ctccactatc agcagcgatg tgatccaaaa agtttttaca gtaaatagcc ctgcaaacct
    25921 atctgcgtaa atcatcactc tctatttacc tccactcaat gtcactttaa atgccctgat
    25981 ccccttcttg agttcagcat atccggtgcc ctggttataa acgatgagca aagagccgtc
    26041 cctgttcctt cccgctctta aactgtaagt gcccctcgtg tcatcaggag ataacctctg
    26101 aaatccgata ttcacccaac ccccgggacc gtgcctgtat aagccgatac cgctgtctga
    26161 gaggacaaca agttgctttt tcccgtctcc gtacagatcc gcaaagtcaa acacataagg
    26221 atactcgata gcttcaagac cctccgatat atcggcaaag ccctcaccag tccatttcag
    26281 ggcggccagc gattgcttta cagttccctt gctatccgcg ctcgaaagcc tgaaaaccgc
    26341 cacgtccttt ccatctccgt ccgcatctcc tgctcttact gcaatcgtaa ctttttccgg
    26401 aaaagggttg acttcataac tattgaaatt gctttttccg tctccgaacc agatactctt
    26461 cttgaacatc tgaatcattg tttccggagc cacaactaca tctttaagac cattgccctt
    26521 cagatccccc actgccatgg agtctccata cacatcggtc cctccctcga caaccttcac
    26581 ctcccagcct ttgccctcct tattgatccc gaccagtatg ccctgcggtt tatatcgcga
    26641 atgaaatggt ccctctgaaa aggctatcac atcaggccat ccatcaccat tcacatcggc
    26701 aatctctaca gtcctggagg aaaaatcttc tttcataggg aatgcggatt cgacaaaggc
    26761 tccctgctta ttgttaagaa gaaggattat cctgccggtg tggtttgcga gaactatatc
    26821 aggagcgccg tcccggttta tgtcgcctac tgctatccca gcgtagctat aatcgctgat
    26881 ttcgggaaag cgaaagcttc cctctgtcca tttccccttc ccggggctcc acgagaatat
    26941 aaaggggtga agtttgtccg cctccgcttt ccttggaggc ggagcaacta tatcaaggaa
    27001 acctccgccg ctcatatcag cgagggcaat attctgcctc cagagcccct cgctgggcag
    27061 tcccccggaa atatcggtaa aggttaatgt ttctttatta aatgtttctt tattatcttt
    27121 cgtctctctt gaggttgacg cgcagctcaa gaatagtaaa agagaaaaaa ggagtacgca
    27181 gcatttcagg actcttttca aggtgtctcc tcacttaaaa atagttttct ataaaaaaag
    27241 aggggggtgg atgaaacacc cccccctttt tttgattcat actgcgtaat ttaacagccg
    27301 gtacctgtgc aggtgcagga ctcaaagtta tccgtcgggc tgtttcctgt aatagtattg
    27361 cctccgctat ccggggagca ataatgtctg tgaatcgggt gaacaacatc ccagttgaac
    27421 tgcgtagacg atccatcagc cttctggtgg gcccagccga agaaagaata ctggcctgtg
    27481 gacgaaggga gaagagtgat tatgtgacct gcggtcccgt tgccgtccgt tgtgcccgtt
    27541 aaagtaaccg gtgccgcact gccgacgaag gtatcttttt caatgatctg gcaattagca
    27601 tagccgttgt gcggtccgcc gggacacgga gttccgctga ccacgccaca aacgggcgga
    27661 gcagcaggga atggcgaaaa caacccgcta aggaggttga gggagccgga accgggacaa
    27721 cccgcgccag tgggcgctgc agtaatatcg aatacgttaa actcattggg aatacttacg
    27781 catactgaac tgataccttc ttcctcatca taaatgtcac agctcaagaa cctaccgcct
    27841 actaaaacgc ctgtcgcctt ggtgggcaga tccgccctgc cgaagagaag catccaccag
    27901 gtgtgggtat ccggcccatt attctggaca acatacctcg ggaagatgcg gcttgctgcc
    27961 acagcacgac tgccattctc ctccaattca gtgcctatct cagcaggacc ctccatagag
    28021 ggagcgttaa atcctgatgc aaagccatta ggcagatcgg ttaaatagac ccacgtgaca
    28081 agcctgtcag cgagtaactc atctgagtct atactggcgc caggtccggc gcatgcagga
    28141 gctggctgtt cctgctgata tgtaacatat ccgatccaat actttgcccc gcttatagtc
    28201 tgcgcgggtg gcgcagcggc atacataacg tgacccggcc cacagccgat tcccgccatt
    28261 atacataagc aatgatccgt ttctacatca tgccatgtgg ggcaaaaatg gtggtcccct
    28321 gcaacaccgg agttcttagg ctcatgaaag acagcatgga gcccgaaccc taatgtagtg
    28381 gtgtctgcgg ggtcgacgat cccaaggacg tcagcgattg cccatccggt atccacggtg
    28441 ccgctgtcag cagagcaaat gacaggaata accgcggtct tgccgggtac tgcatcgtca
    28501 acgccatata ctgcataggc cgttccgacg ccgaagag
//
LOCUS       DATKOU010000068         5493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_853365, whole genome shotgun sequence.
ACCESSION   DATKOU010000068 DATKOU010000000
VERSION     DATKOU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853365"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VMU21_03305"
     CDS             <1..939
                     /locus_tag="VMU21_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HUO76582.1"
                     /translation="FGQTGCQLKNIQTIQSWKPSKRGDFTITPYLVDHSGFDALAFLI
                     EGEGKKVFYSGDFRGHGRKQVLFKNILKNPPRNIDYLILEGSMLGRDKGKYETEQDIE
                     RELTTQFKESDNLFFVSCSTQNIDRLVSIYRACIKSGRTFVLDPYAAFILDRLKAVSS
                     NIPQYNWGKNIKVFFVPNSYTERMAENKSLFKFKSAKITYDEIQEKRHKLVVKDSYTT
                     RKIFANKNNLGNSALIYSMWEGYLPQVKPFWDKHSVPIVMIHSSGHAYIEELQAFVNA
                     MKPKWVIPNHTFYPERYPGIFGRNILTIKDGQTVEL"
     gene            960..1163
                     /locus_tag="VMU21_03310"
     CDS             960..1163
                     /locus_tag="VMU21_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HUO76583.1"
                     /translation="MPYVNIKITREGATPEQKAQLIRGVTKLLSDILGKNPQTTVVVI
                     DEVKTDNWGIGGETVTSRRKRGQ"
     gene            1220..2314
                     /locus_tag="VMU21_03315"
     CDS             1220..2314
                     /locus_tag="VMU21_03315"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HUO76584.1"
                     /translation="MNITKGKTTFVLAIIFSLILHLIALAGIRGVSFVSALSPDIFLT
                     SLEKELPKHSPAMLLKKPPLLNNINKAQIETQEEDDSTISEEPGEQDVPTENNDSAPQ
                     EEQTPDMEDQTESPDNAHIADIADNKENHLETLSKQQDRKKAALLKPSREKLYFELYW
                     LGIYVGNATFEAVLNNGELRITSQVHSTPFVSTFYKVEDYAESRIVDGAPVHFSIKQQ
                     EGKYRSNKETIFDQSGKKVTFLNHLKETKEEHTMEGQGLWDVISGFYYLRTHSMETGK
                     TISINVFDSNKFLTAEVTVMGKERIEDVNQKGIDTIIVKPVLNSDGLFKNKGTILVWF
                     TDDEHRIPVKVETEVPVGKIIAKLMSRESE"
     gene            complement(2328..4025)
                     /locus_tag="VMU21_03320"
     CDS             complement(2328..4025)
                     /locus_tag="VMU21_03320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HUO76585.1"
                     /translation="MNNNLSIIPLGGVEEIGLNSTVFEYSDDIIMVDAGLMFPEEDML
                     GVDFVIPDFSYIIENKERLRGIVITHGHEDHTGALPFLLKELGARKGEGNLWGGDIDI
                     PVYGTALTLGLIREKLKEHHLEHIRLIAVKPRDIIQLGIFSVEFMRVTHSIVDGVGLC
                     INTPMGRVVHTGDFKLDPTPVDGQLMDFHKFSEYGEKGTLLLLSDSTNAEKGGFTFSE
                     KEVRRAFEDIFSKAKGRIIIATFASNIHRVQQAIDVAVMFGRKVILCGKSMVSNSQIA
                     LDLGYLKIPPLTWLALEDIDTLADEEVVIITTGSQGEPMSVLSRIATDEHKTIKIKEG
                     DTVILSAKIIPGNERSIGKIINHLFRRGANVIYEKVSEIHVSGHASKEELKLMLNMVR
                     PKYFMPIHGEYRHLSYHGMLAEKTVVPKENIFILENGEILEISDAGAAKRGRVNSGRV
                     FIDGKGMGDVEDMVLRDRRRLAHDGIVLIHIAVEKLTGNIISGPDIMSRGFIFEDASP
                     DVMNDVKELVSKTLKEMDRELITDASLLQAKLRSVLKKYLRNTMERRPMIMPIIVEI"
     gene            complement(4069..4974)
                     /gene="argF"
                     /locus_tag="VMU21_03325"
     CDS             complement(4069..4974)
                     /gene="argF"
                     /locus_tag="VMU21_03325"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HUO76586.1"
                     /translation="MKRDFLTIWDLSADEIRSIVDRALKLKRGEDRNTCPLIGKSVGL
                     LFEKSSTRTRVSFETGIYQLGAQALFMNASELQMGRGETTADTARTLSRYLSAIVMRT
                     YEHSRVEEFASYATATVINGLSNRHHPCQVLADLLTIQEGKGRLKDIRLAYIGDGNNV
                     ANSLIEAASRMEIHFVMASPEGYEPDPDVLERARAGARSEIIMLRDPREAAGMADVIY
                     TDVWVSMGQEKEAEERKRKFRPYQLNESLLSCAKKDVIVLHCLPAHRGEEITDEVMDG
                     PHSVVFDQAENRLHSQKALMEFLLT"
     gene            complement(4971..>5493)
                     /locus_tag="VMU21_03330"
     CDS             complement(4971..>5493)
                     /locus_tag="VMU21_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUO76587.1"
                     /translation="TGMGRTGKLFAYEHFGIKPDIMSLAKGLGGGFPIGAMLATNAVA
                     AAFRPGNHATTFGGNPLGCAAAIATLETLLEDGFILDQCRRMGEYFIEGLLQLKKEHL
                     SVITDVRGMGLLIGMELTTECGSIVKACLERGVIINCTAGNVLRFTPPLIVRERDIDH
                     VLDILDDLLGRLS"
BASE COUNT         1465 a         1386 c         1291 g         1351 t
ORIGIN      
        1 ttcgggcaaa caggatgcca gttaaaaaat attcagacaa tacagtcttg gaaaccatcc
       61 aagagagggg atttcaccat caccccctat ttggttgacc attcgggatt cgatgccctt
      121 gcttttctaa tagaggggga aggaaagaag gtcttttatt ctggagattt cagagggcat
      181 ggaagaaagc aggtcttgtt taagaatata ctcaagaatc ctccaaggaa tattgattac
      241 ctgattcttg aggggtctat gttgggtagg gataaaggga aatatgaaac tgagcaagat
      301 attgagagag aactgactac ccaatttaag gagagtgata atttgttttt tgtttcctgt
      361 tctacccaaa acatcgacag gctggtatcc atatatcgtg cctgtatcaa gagtggcaga
      421 acttttgtgc ttgacccgta tgcagccttt atcttggata gactcaaagc agtatcttca
      481 aacatccccc agtataactg gggcaagaac attaaggtct tctttgttcc aaatagctat
      541 acagagagaa tggctgagaa caagagcctt ttcaagttca agtctgccaa gataacctat
      601 gatgaaatac aagagaaaag gcataagctg gtagtcaagg attcatacac cacaaggaag
      661 atatttgcca ataagaacaa tctcggcaat tccgctctta tttattccat gtgggaagga
      721 tatttgccac aagtgaagcc attttgggat aaacatagtg tcccgattgt catgattcat
      781 tccagcggtc atgcgtatat tgaagaatta caggcttttg tcaacgcaat gaagcccaag
      841 tgggttatcc ccaaccatac cttctacccc gaaagatatc ccggcatatt tggaagaaat
      901 attttaacca taaaagatgg tcagacagtg gagctataat aatcggaaag gagaatccta
      961 tgccatatgt gaatatcaaa atcaccaggg aaggtgcaac acctgagcag aaagcgcagc
     1021 tcattcgggg agtgacaaaa ttactttcgg acattttggg caagaacccc cagaccacgg
     1081 tggtggttat tgatgaagtg aaaacagaca actggggaat cggcggagag actgttacct
     1141 caagaagaaa gcggggacag tgagaaaggg agagttgatg ttctgaccgt agaggtgtaa
     1201 taatactgtt aaggtctgta tgaacatcac aaaaggcaag accacatttg tactcgcaat
     1261 cattttctct cttatcctcc atctcatcgc ccttgcgggc atcaggggag tctcttttgt
     1321 aagtgccctc tcccccgaca ttttcttgac aagcctcgaa aaggaactac cgaaacattc
     1381 tccggcaatg cttttgaaaa agcccccttt gctcaataat atcaacaaag cgcaaatcga
     1441 aacccaggag gaggatgaca gcactatttc cgaagagccc ggcgaacaag atgtgcccac
     1501 agaaaataac gattctgcgc ctcaggaaga gcaaacccct gacatggagg atcagaccga
     1561 gtcgccggat aacgcccata tagcggatat cgcagacaat aaagagaacc atctggagac
     1621 cctttcaaaa cagcaggaca gaaagaaagc tgcactcctg aaaccttcga gggaaaaact
     1681 ctattttgag ctttactggc tcggcattta tgtgggcaat gccacatttg aggcggtgct
     1741 taacaacgga gagctgagga taacctcgca ggtccattcc actccatttg tttccacttt
     1801 ttacaaggtg gaagactacg cggaaagcag gatcgttgac ggcgcgccgg tccatttcag
     1861 cattaaacag caagagggaa aatacaggag caataaagag accatatttg accagagcgg
     1921 aaagaaggtc actttcttaa atcatctcaa agaaaccaaa gaggaacaca caatggaagg
     1981 acagggcctc tgggacgtta tatcagggtt ttattatctg cggacccatt ccatggaaac
     2041 aggcaagacc atctccatca atgtgttcga cagcaacaag tttctcaccg ccgaggtaac
     2101 cgttatggga aaagagcgga tcgaggacgt gaaccaaaag gggatagaca ccataatcgt
     2161 gaaacccgtt ctgaactctg atgggctttt caagaacaag ggaaccattc tggtctggtt
     2221 caccgacgat gaacacagga ttcccgtaaa ggtagagaca gaggtcccgg tgggaaaaat
     2281 tatcgcgaaa ttgatgagcc gggaatcgga gtgaaaagga gaggtcctca gatttcgaca
     2341 atgatgggca taatcatggg tctccgctcc atggtgttcc tgagatattt tttaagcaca
     2401 cttctcagct ttgcctgaag cagtgaagca tcggtaatca actccctgtc catctctttc
     2461 agcgttttgc tcacaagctc cttcacatcg ttcatcacgt caggtgaagc atcctcaaag
     2521 atgaatcccc ttgacatgat gtcaggacca gagatgatat tcccggtgag cttttccacc
     2581 gcaatgtgga tgagcacaat gccgtcatgg gcaagtctcc gcctgtcccg cagcaccatg
     2641 tcctccacat cccccatgcc ttttccgtca ataaagaccc ggcctgaatt caccctgcct
     2701 ctctttgccg cacctgcatc agaaatctca aggatctctc cgttttcgag gataaagatg
     2761 ttctccttgg gtaccacggt cttttccgcg agcatgccat ggtaagacag gtgacggtac
     2821 tccccgtgga tgggcataaa gtatttcggt ctcaccatat tgagcatcag cttaagctcc
     2881 tcttttgagg catgccccga gacatggatt tcagaaactt tttcatagat gacattggcg
     2941 ccccgcctga agaggtggtt gatgatcttt cctatagagc gctcattgcc gggaattatc
     3001 ttggccgaaa ggattacggt atcgccctcc tttatcttta tggtcttatg ctcgtcagtg
     3061 gcaatccttg aaagcacact catgggctct ccctggcttc cggtggtgat gatcaccacc
     3121 tcttcgtcag ccaatgtgtc gatatcttcc agtgcaagcc atgtaagggg aggaatctta
     3181 aggtaaccga ggtcgagggc tatctgggaa ttagagacca tgctctttcc gcacaggatg
     3241 acctttcttc cgaacatgac cgccacatcg atggcctgtt gcaccctgtg aatattggat
     3301 gcaaaggtcg cgatgatgat ccttcccttt gccttggaaa agatgtcctc aaaggccctg
     3361 cgaacctcct tttcggaaaa ggtgaaaccg cccttttccg catttgtgct gtcagagagc
     3421 aggaggagag tgcccttttc cccatactca gagaatttat gaaaatccat taactggccg
     3481 tccacagggg tggggtcgag cttgaagtca cccgtatgca ccaccctccc catcggggta
     3541 ttgatgcaga gtcccacccc atccacaatg ctgtgagtaa ccctcatgaa ctccacagaa
     3601 aaaatgccca gctggattat gtccctgggt ttgacggcaa tgagtcttat atgttcgaga
     3661 tggtgttctt tgagtttctc cctgataaga ccgagggtga gcgcggtccc gtataccggg
     3721 atatctatgt ccccgcccca aaggttcccc tcacctttcc gggcacccag ttctttcaaa
     3781 aggaaaggaa gggcgcctgt gtggtcttca tggccgtggg tgatgacgat tccccttagc
     3841 ctctctttgt tttcgatgat ataggagaag tcagggatga cgaaatcaac accgagcatg
     3901 tcctcttcag gaaacatgag gccagcatcc accatgatga tgtcgtcgga gtattcgaat
     3961 actgtggagt tgaggcctat ctcctcaact ccgccaaggg ggattatgga gaggttatta
     4021 ttcaactctg tgtatccgca tgttctatta aggatcgaca aaggagcatc aggtcagcag
     4081 aaactccatt aaagccttct gggaatggag cctgttttcc gcctggtcaa aaaccacgct
     4141 gtggggcccg tccatcacct catccgtaat ctcctctccg cgatgggcag ggagacaatg
     4201 gagcacgatc acatcctttt ttgcacatga gagcaatgac tcattcagct gataaggcct
     4261 gaatttcctc tttctctctt cagcctcctt ctcctgcccc atgctcaccc acacgtccgt
     4321 gtagattaca tccgccatac ctgccgcctc ccttgggtcc ctgagcataa tgatctcgct
     4381 cctcgcaccc gcccttgccc tctcaaggac atcagggtcc ggttcgtaac cttcaggaga
     4441 tgccatcaca aaatgaatct ccattctgga ggcagcctca ataagggaat ttgccacatt
     4501 atttccgtca cctatgtagg caagacgtat gtctttaagc cttccttttc cctcctgtat
     4561 cgtaaggagg tcggccaaaa cctggcacgg atggtgacgg tttgaaagac cattaatgac
     4621 cgtagcagtc gcgtaggaag caaattcctc aacccttgaa tgctcatatg tcctcatgac
     4681 aatggcgctc aaataacggg aaagggtcct tgcagtatca gcggttgtct ccccccttcc
     4741 catctgaagc tccgaggcgt tcatgaagag ggcctgtgcc cccagctggt aaatccctgt
     4801 ctcaaaagac accctggttc tcgtggaaga cttttcgaac aagagtccta cactcttgcc
     4861 tatgagagga catgtgttcc tgtcctcacc cctcttcagc ttcagcgccc tgtccacgat
     4921 ggagcgtatc tcatctgccg agaggtccca aatcgtaagg aaatcccttt tcatgaaagc
     4981 cttcccaaca ggtcatcgag tatatcgagc acatggtcta tatccctctc ccggacaata
     5041 aggggaggag tgaaccgcag cacattaccc gcagtgcaat tgatgatcac accccgttcc
     5101 aggcatgcct tgacgatact cccgcactct gtggtgagtt ccataccgat caggaggccc
     5161 atacccctga catcagtgat gacgctcagg tgctcctttt tgagctggag aagtccctca
     5221 atgaaatatt cacccatcct tctgcactgg tcgaggatga acccgtcctc gagaagggtt
     5281 tccagggtgg ctatcgcagc ggcacagcca aggggattcc ctccgaaagt ggttgcgtgg
     5341 tttcctggcc ggaatgccgc agcaaccgca ttcgtggcga gcatggcgcc tatcggaaat
     5401 cctccaccga gccccttggc aaggctcata atatcaggct tgatgccaaa gtgctcatag
     5461 gcaaagagtt tacccgttct ccccataccg gtc
//
LOCUS       DATKOU010000069         6073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1617169, whole genome shotgun sequence.
ACCESSION   DATKOU010000069 DATKOU010000000
VERSION     DATKOU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617169"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VMU21_03335"
     CDS             complement(<1..750)
                     /locus_tag="VMU21_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76588.1"
                     /translation="MSKTTRNVTLISASISVLLLFYVCFVLVANYKSQIALQQTILKQ
                     FSFENERLANTLSNFFNSRKEDLINLAASRLIDVFFENKALGMSMEYGLKQSIPPIKQ
                     RFYALLNRYKTDKKSLYLRIALVDEHGNILSEAYPTDSRPQILSEAKHFLSPEQDKPT
                     FIPTNKGKELIVSLAYYFKGKYSGQIVAWLQPEYLKVVSMLDSSSSHRTSFLIGDDLT
                     IPLSGEAHGLPEIEIMRLGEVHTFQITDKRNK"
     gene            complement(820..1728)
                     /locus_tag="VMU21_03340"
     CDS             complement(820..1728)
                     /locus_tag="VMU21_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUO76589.1"
                     /translation="MNKHCASRKKCYHLFSILAIVLNISLMSGSVFAQDLKDIKQRGI
                     LRHLGIPYANFVTGSGDGMDVEIIKLFALHLGVQYQYVMTDWDNAIGDLTGQKVVPKA
                     SGIEVLGTVPIKGDLIANGMTVLPWRQKVVTFSNSTFPTQVWLVARSDSPLKPITPSK
                     NTEKDIKAVKSLIAERSVLCKKGTCLDPSLYDLQGARAHLEYFEGSLNEIAPALLNKK
                     AELSLLDVPDALVALQKWPGEIKVIGPISDTQDMAVAFRKNSPELMKAFNNFLEKIKK
                     EGTYRKIADKYYPFAADYYPAFFKDR"
     gene            2185..3093
                     /locus_tag="VMU21_03345"
     CDS             2185..3093
                     /locus_tag="VMU21_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO76590.1"
                     /translation="MKLSYPVHTLDNQLLLPAGTVLSTETLEDLISSNKVTSYQAYPL
                     LEYGMVKHDLLRQMIRPPYDLIFSSQERIAGVMTVLEKVNLIIPVLQSLDYFKRDDYH
                     TYCHVLMVLALSTLIARDLLPDYRGWINEVSRGPTHDIGKICIPLHILKKATPLTKAE
                     KSIIDHHSTAGYVLLSYLHKDYKNLAAIVARDHHERKDGSGVPRGIRLRDPMVEIIVV
                     CDVYDALISPRPYRPVSYDNRTAIEELTKMAEMNKIGWKVVKSLVAHNRKAKPRPSEV
                     KVSREKRGTTPSLNFHGVVEEADSDS"
     gene            complement(3211..3363)
                     /locus_tag="VMU21_03350"
     CDS             complement(3211..3363)
                     /locus_tag="VMU21_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76591.1"
                     /translation="MGATDILLMGLIIGGAVYLLYRSLWKKKGHCSGCEVGTCEMKRK
                     YNSKDC"
     gene            complement(3430..5607)
                     /gene="feoB"
                     /locus_tag="VMU21_03355"
     CDS             complement(3430..5607)
                     /gene="feoB"
                     /locus_tag="VMU21_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HUO76592.1"
                     /translation="MSTQIIEDRIQSGDNTPQSPLISRGDKRGVTVAVAGNPNSGKST
                     LINALAGTRLSVGNWPGVTVEKKSAIFEYEGTKIRLVDLPGTYSLSPYTQEEIIAGDY
                     LVNEKPDLVINVVDATNLERNLYLTVQLLELGIPVVIAMNIYDEAVKKGYEIEIKTME
                     EMLGVRVIPTVATKKTGLDDLLKAVTDITDGTFSHQPKKLNYGADIESAASAVEAQIR
                     NSYPKLTEKYPLRWLAFKLMEKDSHTMEEINIGGTISFVDEKTEHLRKAHGEDIESIM
                     ADERYALASGLAHEVLKKPEFRKMEITEKIDKIALNRFLGIPIFLAAMWLVFKLTFDV
                     SKPFADWMNSTASGPFKRWAEVLMSLIKAPDWTASLVTDGVIAGVGSVIVFVPVIFAM
                     MFFITFLEGSGYMARAAFVMDRAMHAIGLHGKSFIPMLLGFGCNVPAVYATRTLENPK
                     DKALTALLIPLMSCGARLPVYVVFIGAFFSAHAGTVLWSLYVMGIVLAVAMGLIFKKT
                     LFRGEAPVFIMELPPYRMPSFKSLMIHTWEKGKHFLVKAGTYILAVSILVWFLLNLPW
                     GVEQKRDSYLGKTGQIIAPVLEPLGFGSWEAASSLISGLIAKEIVVGTMGEIYVTKAE
                     EEKKERPSFREDLRDVVVSFGTAAKAAALNVFSTPGISSLSAEEDEKRAGLKGEIQKA
                     FTPLSAYAFIVFILLYMPCVVVAIAMSRSSARGNGSGSPLHTK"
     gene            complement(5604..5831)
                     /locus_tag="VMU21_03360"
     CDS             complement(5604..5831)
                     /locus_tag="VMU21_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HUO76593.1"
                     /translation="MNLAMLKPGENGRITKMGAVGPLKRRLMDMGVLIGEKIKVEKIA
                     PLGDPIEVTIKNYNLSVRKKEAEGIEVEVVK"
     gene            complement(5831..>6073)
                     /locus_tag="VMU21_03365"
     CDS             complement(5831..>6073)
                     /locus_tag="VMU21_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HUO76594.1"
                     /translation="LGLLSRGDKAEIVEIKGQMGCCHGNSKNQLCHAEDMGLRIGKSI
                     EMLNNEGRGPILLKVDESRIAIGRGMAMKIMARRKE"
BASE COUNT         1553 a         1520 c         1303 g         1697 t
ORIGIN      
        1 cttgttcctc ttatccgtga tttggaatgt atgcacttca cctaatcgca ttatttcaat
       61 ctcaggcaga ccgtgggctt ctccagacag aggaattgtt aagtcatctc ctataagaaa
      121 agaggtccta tgtgaagatg atgaatcaag cattgatacg actttgagat actctggctg
      181 cagccatgcg actatctgac cagaatattt ccccttaaaa tagtaggcaa gagaaacgat
      241 aagctccttc cccttatttg ttggaatgaa agtcggcttg tcctgttcag gagaaagaaa
      301 gtgtttcgct tctgacaaaa tctgaggacg ggaatctgtt ggatatgcct cagaaaggat
      361 gttcccgtgt tcatccacta atgctatcct gaggtacaaa gacttcttat ccgttttata
      421 acgattaagg agcgcataaa accgctgttt aatagggggg atactttgtt tcaaaccata
      481 ttccatgctc atgccgagtg ccttattctc gaagaagaca tcaatcaggc gggatgcagc
      541 aaggtttatt aggtcttcct tgcgactatt aaagaaattg ctcagagtat tggctagcct
      601 ttcattttcg aagctgaact gcttgagaat tgtctgttgc aatgctatct gggatttgta
      661 gttggcaact agaacaaagc agacataaaa aagcaagagc acgcttatgc tagcactaat
      721 caaggtaaca ttcctcgttg tttttgacat agtgtttatt ttctcattgt gacgtcaaaa
      781 agcctcgcca ttctctaagc caaggagaga tagatcccac tatctatcct taaaaaaagc
      841 aggatagtaa tccgcagcaa aaggataata tttgtcggca atcttccgat acgttccctc
      901 tttttttatt ttctcaagaa agttgttgaa ggccttcatg agttcaggag aattcttcct
      961 aaatgccaca gccatatctt gcgtgtcgct gatcgggcca atcaccttaa tttcccctgg
     1021 ccacttctgc agggctacaa gagcatcagg aacatccaga agtgataatt ccgctttctt
     1081 attgaggagg gcaggagcaa tctcatttaa tgaaccttca aaatattcaa ggtgtgctct
     1141 cgcgccctgg agatcataca atgatgggtc taagcaggtc ccctttttgc agagaacaga
     1201 gcgttctgca attaatgact taactgcttt tatgtctttt tccgtattct tgcttggcgt
     1261 tatcggctta agtggtgagt cagaacgtgc gacaagccat acctgggtag gaaatgtaga
     1321 atttgagaat gttactacct tttgtctcca aggcaacaca gtcattccat ttgcaataag
     1381 atcgccttta ataggaacag ttcctaaaac ttctatgccg cttgctttcg ggactacttt
     1441 ctgaccagtc agatctccga tggcattgtc ccagtctgtc atgacatatt gatattgaac
     1501 tccaagatgg agggcaaaga gtttaataat ttcgacatcc attccatctc cacttcctgt
     1561 cacgaaattg gcgtatggga taccaaggtg acggagaata cctctttgct tgatatcctt
     1621 aagatcttgt gcaaaaacag atcctgacat cagagagata ttcaaaacaa tcgcaagtat
     1681 agaaaagaga tggtaacact tctttctgct agcacagtgc ttattcatag tgcctccttc
     1741 tgccgttcta tggccccgca atacacgagg cctttcttat acattgtgcc cgggcgaatg
     1801 cttcaggctt ccccactgcc ttatgtaggg cttgcacacc acattcagca cgacgggatc
     1861 tctaacacat agtgttctct cgacctctaa acccaaggca agtcctatgc ttgattacag
     1921 tgtgctgttt agtgccttca aagtcaatcc tacctgagta ggatataaaa ctgtttagtc
     1981 ttatgttatt atcctgatga taaccccatt tcttcctgaa acaaacacgt tcaaaatgta
     2041 gattatcatg gtgattacta ctgcaatgaa tataggggag cacccttaaa agagcctttc
     2101 taatatactt ttttcttgac atcatgctgt tgaaaaaaag tcaagcatct gcgtaatata
     2161 cagcaagaaa cctcttagga tgctatgaaa ctctcttatc ctgtgcacac attggataat
     2221 cagcttttgc tgccggccgg caccgttctt tcaacggaga cgttagagga tttgatctct
     2281 tcaaacaagg taacatctta tcaagcctat ccattgctcg aatatggaat ggtaaaacat
     2341 gatttgctcc gtcaaatgat caggccccca tacgatttga ttttctctag tcaggagaga
     2401 attgcaggag tcatgaccgt gctggagaag gttaacctca tcattcccgt tttgcagtca
     2461 ctggattact ttaagagaga cgactaccat acatattgcc acgttcttat ggtacttgct
     2521 ctttctactt taattgctag agatctgtta cctgattatc gggggtggat aaatgaagtt
     2581 tcgcgtggcc ctactcatga tattggcaaa atctgcattc ctttgcatat cttgaaaaaa
     2641 gccacccccc tcactaaggc tgaaaagagc attatagatc atcattctac tgcaggctat
     2701 gttcttctca gctatttgca caaggattat aagaaccttg ccgccatagt ggcgagggat
     2761 catcacgaga ggaaagatgg ttccggcgtg ccccgtggga tacggctcag ggaccccatg
     2821 gtggagataa tagttgtgtg tgacgtatac gatgctctta tttctccgag accttaccga
     2881 cctgtctcat atgacaacag gacagcgata gaggagctca ctaaaatggc tgaaatgaat
     2941 aagatcggat ggaaagtagt caaatctctg gtggcacaca accgcaaggc taaacctaga
     3001 cccagcgagg taaaagtctc aagagaaaaa aggggcacca ccccttcgct caatttccac
     3061 ggagttgttg aagaagctga cagtgactcc tgacaaaata atccttttag atttatccga
     3121 ttgaaattac actttagtga gctggatgcc cttttctaga ggccggcctt ttatcgagca
     3181 aatcgaagga tagagttgtc attttctgtt ttaacaatct ttcgaattat attttctttt
     3241 catctcacat gtcccaacct cgcatccgga acaatgccct tttttcttcc agagggagcg
     3301 gtacagaaga tataccgctc caccaataat cagtcccatc aataaaatgt ctgttgctcc
     3361 catcttagcc tcctataccc agtaattttc caccttggta gatcacaaat gccactaccc
     3421 aggcaagaac tatttggtat gcaaaggcga ccccgaacca tttccacgtg ccgaactcct
     3481 gctcatggcg attgcgacaa caacgcaggg catatagagg aggataaaga cgatgaatgc
     3541 gtaagcgctc aagggtgtga aggccttctg tatctcccct ttaaggccgg ccctcttctc
     3601 gtcctcttca gcagagagac tggatatccc gggggtcgag aagacgttca atgcggcggc
     3661 cttcgcagcc gtaccaaagg agacgacaac gtccctcagg tcttccctga aagaaggccg
     3721 ctccttcttc tcttcttcag ccttcgtgac atatatctca cccattgtcc caaccacgat
     3781 ctctttggcg atcaggccgg agatgagaga ggaagccgcc tcccagcttc cgaaaccgag
     3841 gggttcgagc acgggagcta tgatctgccc cgtcttcccg aggtaagagt ccctcttctg
     3901 ctcgacgccc cacggcagat tgaggagaaa ccagacgagg atcgagaccg ccaggatata
     3961 ggtccctgcc ttgacgagga agtgttttcc cttctcccac gtatgtatca tgagactttt
     4021 gaaggacggc atcctgtaag gcggaagttc catgatgaag acaggagcct ctcccctaaa
     4081 aagcgtcttc ttgaagatga gccccatcgc tactgccagc actataccca ttacgtacaa
     4141 cgaccagagg accgtcccgg catgtgctga aaagaaggcg ccgataaaaa caacatacac
     4201 ggggagccgc gcgccgcagg acatgagtgg aatgagcagg gccgtgagcg ccttgtcctt
     4261 ggggttttcg agagtcctcg tggcgtaaac ggccggcaca ttacagccga aaccgaggag
     4321 catcgggatg aaggacttgc cgtggagccc gatggcgtgc atcgccctgt ccataacaaa
     4381 ggcggccctc gccatatagc cgcttccttc gagaaaggtg atgaagaaca tcatggcgaa
     4441 gatcaccggg acgaatacga tcaccgatcc gacgcctgca atcaccccgt cggtcacaag
     4501 ggaggccgtc cagtcggggg ccttaataag gctcatgagg acttcagccc atctcttaaa
     4561 aggccccgag gccgtcgagt tcatccagtc ggcaaagggc ttcgagacat caaaggtgag
     4621 tttgaacaca agccacatgg cagcaaggaa tatgggaatg cccagaaacc tgttaagtgc
     4681 tatcttgtct attttttctg ttatttccat ctttctgaat tcaggctttt ttaagacttc
     4741 atgggcaagt cctgacgcca gggcgtacct ttcatctgcc atgatggatt caatatcctc
     4801 accatgcgcc tttctgagat gttctgtctt ttcatcgaca aaggagatag tgcctccaat
     4861 attaatctct tccattgtgt ggctgtcctt ctccattagt ttgaaagcaa gccatctcaa
     4921 cgggtacttt tctgtaagct tcggatagct gtttctgatc tgagcctcta ctgccgaggc
     4981 ggcagattca atatcagcac cgtaattcaa tttcttcggc tgatgactga atgtgccgtc
     5041 tgtaatatcc gtgactgcct ttaacagatc atccagccct gtcttttttg ttgcgactgt
     5101 cggaatgacc ctgacgccga gcatctcttc catcgtcttt atctcaatct cataaccctt
     5161 ttttaccgcc tcgtcataga tattcatggc aatcacaacc gggataccga gttcaagcag
     5221 ctggactgta aggtagaggt tcctttcaag gtttgtggca tccaccacat ttatgacaag
     5281 atcaggtttc tcattgacca gatagtcccc tgctatgatt tcttcctgcg tataagggct
     5341 caggctgtac gtgcccggaa gatctaccag cctgattttt gtgccttcat attcgaatat
     5401 cgccgatttc ttctccaccg taaccccagg ccaattacca actgaaagcc tggttcctgc
     5461 aagcgcattt atcaaggtcg acttgccgga gttggggttg ccagctactg caacggtgac
     5521 cccccttttg tccccccttg aaataagggg ggattgagga gtgttatctc cgctctgtat
     5581 tctgtcttca atgatctgcg tgctcatttc accacctcca cttcaattcc ctcagcctct
     5641 tttttcctga cggagaggtt atagtttttt atagtcactt caatggggtc tccgaggggg
     5701 gctatcttct cgacctttat cttttctcct atcagcaccc ccatgtccat cagccttctt
     5761 tttagcgggc caaccgcccc catcttcgtt attcttccgt tctcacccgg ttttaacatc
     5821 gccaagttca ttattctttc ctcctcgcca ttatcttcat cgccatcccg cggcctattg
     5881 caatcctgga ctcatccact ttcaggagaa tggggccccg gccctcattg ttcagcattt
     5941 ctatactctt cccaatacga agccccatat cttctgcatg gcataattga ttcttgctgt
     6001 ttccatggca gcagcccatt tgtcccttta tctccacaat ttcagcctta tcacctctgc
     6061 ttagaagccc aag
//
LOCUS       DATKOU010000070        14778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1237494, whole genome shotgun sequence.
ACCESSION   DATKOU010000070 DATKOU010000000
VERSION     DATKOU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 14778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14778
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237494"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..2344
                     /locus_tag="VMU21_03370"
     CDS             242..2344
                     /locus_tag="VMU21_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUO76595.1"
                     /translation="MDRERKDRPNRLINEKSPYLLQHAYNPVDWYPWGEEAFEVARRD
                     NKPVFLSIGYSTCHWCHVMAHESFEDPEVARLMNDAFVCIKVDREERPDIDSVYMNVC
                     QLMTGSGGWPLTIVMTADKRPFFAGTYFPNENRYGRIGMRELVPRIKGIWLSDPAKIT
                     ESAERVTTALRDLSVRSTRDELNETVLEACYRDLQERFDAVHGGFGTKPKFPTPHNLL
                     FLLRMGKHGKLAHAGEMVEKTLLQMRMGGVYDHIGYGFHRYSTDEGWLLPHFEKMLYD
                     QAMLLIAYCEAYQATKNETFRNTANEICAYVLRDMTMDSGGFYSAEDADSEGVEGKFY
                     LWSQEEISGILTKDEAELVRKVFNSESSGNYHEEATGEKTGSNILHMNKALSEIAAEL
                     GMSHEACEGLLFSAKEKLFTSRKGRIHPHKDDKVLTDWNGLMIAALSIAARVLGDESY
                     QRAAEKAAQFIMHTMRTNDGRLFHRYRDGEKGIAGYLDDYAFMIWGCIELYETGFDMD
                     FLEFALNLCETLNSRFLDPENGGYFLTPDDAERLPVRTKEVYDGAVPSGNSVMMYNLL
                     RLSHIMGKPHYYDLALGIEKAFSEGVRESPAAHTMLLAALGFRLWPVSEVVIAGDLKG
                     EDTQAMLLALNSEFVPNKVVSVREAGSRAPGIEGIAGFALNLSPDEQRATAYVCINRS
                     CHPPTTDPVKMMDLLNPS"
     gene            2475..3050
                     /locus_tag="VMU21_03375"
     CDS             2475..3050
                     /locus_tag="VMU21_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HUO76596.1"
                     /translation="MKSVKGTKTEKNLLTAFAGESQARNRYTYFASQAKKEGYEQIAA
                     LFIETADNEKEHAKRFFKFLEGGDVEVVAAFPAGIIGTTGQNLSAAAADERYEHTHMY
                     PEFARIAEEEGFPLIAGVFRSVATVERQHEKRYLALRANIERDRVFRREAPAAWKCRN
                     CGYVYEGSEAPDKCPACDHPQSFFELFEENY"
     gene            3072..3620
                     /locus_tag="VMU21_03380"
     CDS             3072..3620
                     /locus_tag="VMU21_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HUO76597.1"
                     /translation="MAEEKKETWLNYLALTTVIFAVCATLSTFKGGGFSTRSVLAQTQ
                     ASDQWAYYQAKSIKGYIYETQKEKFELELKGSGSKMPRSMVEDYTQRIDSYSRKIKKY
                     EEEKKQIEQEARKFESVKDDAQRHAQIFGMAVIFLQIAILLSSIAALLKKKIVWVLGL
                     FLGVAGLVYFMNGFFLFIQIAI"
     gene            3851..6250
                     /gene="gyrA"
                     /locus_tag="VMU21_03385"
     CDS             3851..6250
                     /gene="gyrA"
                     /locus_tag="VMU21_03385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUO76598.1"
                     /translation="MPINIEEEMKVSYLDYAMSVIIGRALPEVRDGLKPVQRRILYAM
                     FREGLLPGKKYSKCAGVVGEVLKKYHPHGDTAVYDALVRLAQDFNMRYPVVDGQGNFG
                     SIDGDPPAAYRYTEARLAKIAEELLADIDKETVDFIANFDETTEEPKVLPSRIPNLII
                     NGSAGIAVGMATNIPPHNLGEIIDALVMILENDETTAADLMTVVKGPDFPTGGIIHGT
                     TGIKDAYENGRGIIKIRARARIEREHKGGEKIIISELPYQVNKARLIEKIAELVREKK
                     IEGVSDIRDESDRDGIRVVLELKRGEMAEVVLNNLYKHTQMETTFGIIMLALVGGQPH
                     VLALKRLLNYFLQHRRDVVLRRTRYELRKAEERAHILEGLKIALDHLDEIIALIRASK
                     SPEEARQGLMKNYPLTEIQAQAILDMKLQRLTGLERDKIITEYKDTLKEIERLKAILG
                     SAALVSKIIKDELLEIRTRYADERKTEITEETKEITIEDLITEEEMVITLSHQQYIKR
                     NPLSAYRSQRRGGKGLMGMETKEEDFVEQLFIGSTHDYMLFFSNLGRLYWKKIYEIKE
                     FGRAAKGQALVNLLSLAEGERIATALPVRDFKEGYLLTFTKKGMVKKTVLDEYSNPRG
                     KGIIAVTLEEGDELITVRKTDGKSDLIIGTSEGLSIRFNEEDVRPMGRSAKGVIGIRL
                     QKGDEVVSAEVAEMNTALLTVTEKGIGKRTKLEEYRVQGRGGRGVISIKLTEKGGKAV
                     GLMQVRDEDEVVMIANTGKLIRTVAGNISIHGRNTQGVKLMDLDPEDRIVSIGRVAEK
                     D"
     gene            7351..8613
                     /gene="ltrA"
                     /locus_tag="VMU21_03390"
     CDS             7351..8613
                     /gene="ltrA"
                     /locus_tag="VMU21_03390"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HUO76599.1"
                     /translation="MKQVGVMGRIYEPGRLYNAWQQVKKNAGAAGIDKMTVRDFDSRE
                     TELLGLIHEKLKTGTYRFKPARRVLIPKEGTSKMRKLGIPAVMDRIVSQSVNSVFAEI
                     FDPDFTASNFGFRKGRSQHMAIRYVQAIVREGCEWCASIDLKSFFDEIPHSLILKLIR
                     RKIADERLLTLIARALKAGVIVEGRFEKTEKGCPQGSPLSPMISNIVLNELDQELEHR
                     GLKYARWADDFVILLRSERAATRVLDGITNYLKENLGLPVNEAKSGVTETKKVTFLGF
                     QILAGKIRVSDKARTKFKDKIRELTRRNNPLSMSQLIEDINRYMQGWISYFSIQEFKY
                     LFRDFDAWIRSRLRSVQLKKWKNPRKFQRIAIKSGLRPQEARRTWLRMNKWQSTNRKE
                     VRFVMNLAWFRKLGLVFLYDSTPATFHP"
     gene            complement(8717..9304)
                     /gene="hisB"
                     /locus_tag="VMU21_03395"
     CDS             complement(8717..9304)
                     /gene="hisB"
                     /locus_tag="VMU21_03395"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HUO76600.1"
                     /translation="MRKSTVQRKTRETAISMELNLDGRGKYSIKSPVPFLDHMLSLMC
                     KHGLLDMSLRATGDIEVDYHHTVEDIGIVLGKALKQSLGTMEGITRYGHASVAMDEAL
                     ASLSLDMSGRPYLVYNVTLPKKARIKDFDADLVEDFLQAFTSHGGVTLHVNVPYGRNI
                     HHIIEAIFKGLGRALREAVTIDPRVKGLPSTKGKL"
     gene            9462..10067
                     /locus_tag="VMU21_03400"
     CDS             9462..10067
                     /locus_tag="VMU21_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76601.1"
                     /translation="MKRLKVFLFTIMVVGLGSFSFAEGADAQFYFDRGSSFLNEGQFD
                     KAIKDFSEALSQNPKITEAYYNRGQAYYRKGQYENAIADFNKVIEVFPDNADIYNLRG
                     LAYFKTGQYQNAVDDETRAISLNPSFVGALHNRGIAYARTGQYDKAIEDYNKAITLKP
                     DYMDAYYSRGVAYVRKASSDFTRACEKGNKFACENLKQISQ"
     gene            10124..11041
                     /locus_tag="VMU21_03405"
     CDS             10124..11041
                     /locus_tag="VMU21_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO76602.1"
                     /translation="MSALISRFPVHTLDHQQLLPAGAEVSGETLDALISTRVAVSYDS
                     LSLLQHESVKEDILTFLCTPPCDIIFSDPQEVTELMHLMDTVHLALPFLQSLDYFKQY
                     DFYTYRHILMVFAFSTLLAKDLLPDYKDWIQQAATGPTHDFGKICVPLEILKKNTPLT
                     RAERSIIEHHTTAGYVLLCYYLGGPHHPAARVARDHHERRDGSGYPCGVTLMDLLVEV
                     ISVADVYDALISPRPYRVASYDNRTALEEITLLAEKNKISWDVVRALVAHNRKEKPHF
                     SQCDVSYEKRGKPPKGNVHGILAEDSENK"
     gene            11102..11686
                     /locus_tag="VMU21_03410"
     CDS             11102..11686
                     /locus_tag="VMU21_03410"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HUO76603.1"
                     /translation="MNYLIIYAHPNPGSFNHAIMEAVTEELRHHGRGVKIRDLYQMEF
                     NSILKSEDFANMQRGSVARDVKEEQELIRSAEVMIFVYPLWWAGMPAMLKGYIDRVLT
                     EGFAYRISDEGIAGLLAGKKVFLFTTMGASMGDYEESGFFKSMGQITDTGVFAFCGCE
                     VLGHKYFPSVPYVTDSDRKKMLEEVRELVKQNLL"
     gene            complement(11663..11863)
                     /locus_tag="VMU21_03415"
     CDS             complement(11663..11863)
                     /locus_tag="VMU21_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76604.1"
                     /translation="MKVAIKTQILPYPGFTSAEDYHQKFHFTDEYVLMEEFKKEVDSY
                     GLVHPSCLFTPGNSISQEILFY"
     gene            12080..12697
                     /locus_tag="VMU21_03420"
     CDS             12080..12697
                     /locus_tag="VMU21_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUO76605.1"
                     /translation="MGTIERREREKRDMKGLILKTAMRLFLEEGFEKVTIRRIAEEIE
                     YSPATIYLYFADKDEILFALHEQGFEKLHKLQQTVLSIKDPLERLRRHGKVYITFALK
                     QPEYYDLMFIMRSAARKLKEDEEWTAGRRSYEFLRENVGKCIEAGYLPGGDLDIAAFS
                     VWSLVHGIVSLIIRGRCSMFPQEHLAAIAEGALEYSMSKIGTGQS"
     gene            12763..13518
                     /locus_tag="VMU21_03425"
     CDS             12763..13518
                     /locus_tag="VMU21_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HUO76606.1"
                     /translation="MEKKSILAISGSMRNPSFTERMLDLCVEGMGEGFEVRKFYPHRM
                     KIRPCLGCWTCWKKTSPGVCVQKDDFNQILSAYKEADYVLLAAPLYFFGLPATVKNVI
                     DRLFVILEPAQYKSLRGGTEHPKRYGRHPKAVIISSCGFPEMESFDLLRQHFRKICTE
                     LEWKWAGEVLVPGAGTANAPRLFDRKYRLIRQAGEELATGEIRPDTTERIASSVMPHE
                     DYRKMCTAGFRGDILGKLICILIATKAMLKQAL"
     gene            complement(13644..14069)
                     /locus_tag="VMU21_03430"
     CDS             complement(13644..14069)
                     /locus_tag="VMU21_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76607.1"
                     /translation="MDKIICVPIYKAPVIDTLINWYNTFIGVRGKYFMECPRCGEEIT
                     DGTECKKCRKGAVPPKEMEIQYKEFKISELLDIRMTSHISSGEEIEKSEFVPEKGDGS
                     NRAARASKEPYDKKTLLVVTVVISLLAVIAGFYLLRFLF"
     gene            14240..14563
                     /locus_tag="VMU21_03435"
     CDS             14240..14563
                     /locus_tag="VMU21_03435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76608.1"
                     /translation="MFENYRRYQRYSVSAKAVIRRLIAESQKGFTTRVNTISQGGMGF
                     YTDVFMEKATPVSIEFLFGALQVREEDILVGRIASVSSQEKDYFVGVSFEREISYDRF
                     MEIVG"
BASE COUNT         4006 a         3407 c         4029 g         3336 t
ORIGIN      
        1 caggcagcgt cggcaggcaa ggctggggta tctatcccct gtggcatatg aacagaaatt
       61 ctatacagga cagctggcag cttgaaaggt ttggtgtcca ctattgacat ccgaggtcac
      121 taccagtaat tgtcgcctgg attcaaatgg gcgcagaacc tcgttgcaaa aggtgacaaa
      181 aggttctaca atcaagaaaa ttagaccaaa tcttttttat cctggaagag agaatccata
      241 tatggacagg gaacgcaaag atcggcctaa ccgtctcatc aatgaaaaaa gcccttatct
      301 tctccagcat gcatacaatc ccgtggattg gtatccgtgg ggagaggagg cattcgaagt
      361 tgctcggcgg gacaacaagc cggtattcct ctccatcggt tattcaacct gccactggtg
      421 ccacgtgatg gcccatgaat cctttgaaga tccggaggtc gccaggctca tgaacgacgc
      481 ctttgtctgc atcaaggtgg acagagagga gaggcctgac atcgacagtg tctatatgaa
      541 tgtctgccag ctcatgacag gaagcggggg atggcctctc accatcgtga tgactgcgga
      601 taaaagaccg ttcttcgcag ggacctattt cccgaatgag aatcgctatg gcaggatagg
      661 gatgcgggaa ttggtgcccc gcatcaaggg aatctggctc tccgacccag caaagattac
      721 ggagtctgcg gagcgggtta ccacagcgct cagggacctt tcggtccgct ctacgcggga
      781 tgaactcaat gagacagtcc tcgaagcatg ttacagggac ctgcaagaac gctttgacgc
      841 ggtccacgga ggcttcggaa caaaaccgaa attccccact ccccataacc tgctttttct
      901 cctccgcatg gggaaacacg ggaaactcgc tcatgcgggg gagatggtgg agaagaccct
      961 tttgcagatg cggatggggg gtgtgtatga ccatattggg tatggatttc accgctattc
     1021 aacggatgag gggtggcttc tgccccactt tgaaaagatg ctctacgacc aggcgatgct
     1081 gctgattgcc tattgcgagg cgtatcaggc aacgaagaat gagaccttca ggaacacggc
     1141 aaacgagatt tgtgcctatg tgctccgtga catgacgatg gattccggcg gattttattc
     1201 ggcagaggac gctgacagtg aaggggttga ggggaagttc tatctctggt cccaggagga
     1261 gatttcggga atcctcacca aagacgaggc cgaactggtg cggaaggttt tcaatagcga
     1321 atcctcaggg aattaccatg aagaagccac tggagagaaa acaggcagca atatactcca
     1381 catgaacaaa gcgctttctg aaattgctgc ggaactgggt atgtcccacg aagcctgcga
     1441 ggggcttctc ttttccgcga aagagaaact atttacttcg cgcaagggga ggatccatcc
     1501 gcacaaggac gataaggtat tgactgactg gaacggcctt atgatcgctg cgctctctat
     1561 tgcagcgcgg gtgttgggtg atgagtcata ccagagggcg gcagaaaagg cggcacagtt
     1621 cattatgcat accatgcgta ccaatgacgg gaggcttttc cacaggtacc gcgatggaga
     1681 aaagggaatt gccgggtatc tggatgacta tgccttcatg atatggggat gcattgagct
     1741 ctacgaaaca ggttttgaca tggattttct ggaattcgcc cttaacctgt gtgagaccct
     1801 gaacagccgt ttcctcgacc cggagaacgg aggatatttt ctgactcccg atgatgcgga
     1861 gaggcttccg gtccggacaa aagaggtgta tgacggcgcc gtaccttccg gaaattccgt
     1921 gatgatgtat aacctcctca ggctctccca cattatggga aaaccgcact attatgacct
     1981 ggctcttggg atcgaaaaag ccttttctga aggcgtgaga gagtctcctg ctgcccatac
     2041 catgcttctc gcagccctgg gattcagact ctggcctgtg agcgaagtgg tgatcgcggg
     2101 ggatctcaaa ggagaggata cacaggcaat gctcttggcg cttaattctg agtttgttcc
     2161 gaacaaggtc gtatcggttc gtgaggccgg aagcagggca ccgggcattg aggggattgc
     2221 cgggttcgct ttgaatctca gccccgatga gcagcgggcc acggcatatg tgtgcataaa
     2281 ccggagctgc catcccccca caacagatcc ggtgaaaatg atggatctcc tcaacccctc
     2341 ataactgcga gaaccctctc gaaattgtac agaaagatat tctataattg aatgatcctc
     2401 tgcgagcctg tatgcaggtc atcagatatc atataccatg aaccattgga ccattttctc
     2461 tgaaaggcgg acctatgaaa agtgtgaagg gaacaaaaac agaaaaaaat cttctcaccg
     2521 cttttgcggg tgaatcccag gcgagaaacc ggtacactta ctttgcctca caggcgaaga
     2581 aagagggcta cgagcagata gccgcgttgt ttatcgagac agcagacaac gaaaaggagc
     2641 acgcaaagcg tttcttcaag tttctggagg gtggtgacgt ggaggtcgtc gcagcatttc
     2701 ctgcaggtat tataggaact actggacaga acctctctgc agccgctgcg gacgagcgct
     2761 acgagcacac gcacatgtat cccgaatttg cacggatagc tgaagaagag ggctttcctc
     2821 ttattgcggg tgtgttcaga tcagtggcaa cggtggaaag gcagcatgag aagcgctacc
     2881 tcgcattgcg cgcaaatatt gaaagggacc gtgtgttcag gcgtgaggcc cctgcggcat
     2941 ggaagtgccg caactgcggg tatgtgtatg aaggcagcga ggcacccgac aagtgtcctg
     3001 cctgtgatca tccccagtcc tttttcgaac tttttgaaga gaactattaa cacacgaaac
     3061 aggtatacag aatggctgaa gagaagaaag aaacatggct gaattatctt gccctcacca
     3121 ccgtgatctt cgctgtttgt gcgaccctct ccacctttaa gggcgggggg ttttcgaccc
     3181 gttccgtact tgcccagacc caggcctctg accagtgggc atactaccag gccaagagca
     3241 ttaaggggta tatctacgaa acccaaaagg agaagttcga actcgagttg aagggatcag
     3301 gctcaaagat gcccaggtcc atggtggaag actacacaca gaggatcgat tcatattcca
     3361 ggaaaataaa gaagtatgaa gaggaaaaga agcagattga gcaggaagca agaaaattcg
     3421 agagcgtaaa agacgatgca cagagacacg cccagatatt cggtatggcg gtgatattcc
     3481 tccagatcgc gatactcctt tcttcaattg cagccctgct gaagaaaaag atcgtctggg
     3541 tgctcggtct cttccttggt gtggccggat tggtctattt tatgaacggg ttcttcctct
     3601 ttatccagat cgcaatatag cctcttgtta agctcttccc ctctccacag gaaatttctt
     3661 gtccttctga tggtggcgca gtcctgatgg cattgagaca aaatgcctgc gtccccgttt
     3721 tccaggggct tggtccggtg aggggaaagc cctgtcttgg cgttgaaaat aaaagcaaat
     3781 tcgccccctg aaatatggta aaataatcag ttttataaag agaggaaggg gaatacatga
     3841 gatggcaaag gtgccgataa atatagaaga agagatgaag gtgagctacc ttgattacgc
     3901 gatgagcgta atcatcggga gggcgctgcc tgaggtgagg gacggactga aacctgtgca
     3961 gaggaggatc ctctacgcta tgttcaggga aggtctcctc cctggaaaga aatattcaaa
     4021 gtgtgcaggg gtggtgggtg aggtgctgaa gaaataccat ccccatgggg acaccgcggt
     4081 ctacgacgcc ttggtccgcc tggctcagga cttcaacatg agatatcctg tagtggacgg
     4141 ccaggggaat tttggctcta tagacggtga tccccctgct gcctaccgtt acactgaggc
     4201 aaggctggca aagattgctg aggagcttct tgcggacatc gacaaggaga cagtggactt
     4261 catcgcaaat ttcgatgaga ctaccgaaga gccgaaggtc ctcccctcac ggattcccaa
     4321 tctcatcatc aatggctcgg caggcattgc ggtgggtatg gccaccaata tccctcccca
     4381 taacctcggc gagatcattg acgccctcgt gatgattctg gaaaacgatg aaaccaccgc
     4441 agctgatctc atgaccgtgg tcaaggggcc tgatttcccc acagggggca tcatccatgg
     4501 cactacagga attaaagatg cgtatgaaaa cggaaggggg atcatcaaga tcagggcacg
     4561 ggcgaggatc gagcgtgaac acaagggtgg cgaaaagatc atcatctctg aactccccta
     4621 ccaggtgaac aaggcgaggc tcatcgagaa gatcgcagag ctggtgagag aaaagaagat
     4681 cgagggggtt tctgatataa gggacgaatc ggacagggat gggatcaggg tggtccttga
     4741 actgaaacgt ggagaaatgg cggaagttgt cctgaataac ctctataagc atacacagat
     4801 ggagaccacc ttcggcatca tcatgctcgc cctggtgggc ggccagcccc atgtccttgc
     4861 cctgaagcgt ttgctgaact acttcctcca gcacagaagg gatgtggtgc tcaggaggac
     4921 acggtatgag ctaaggaagg ctgaggaaag ggcccatatc ctggagggac tcaagatagc
     4981 actggaccat ctggacgaaa tcatcgccct cataagggcc tcaaagagtc cggaagaggc
     5041 tagacagggg ttgatgaaga attaccctct caccgagatt caggcacagg ccatcctgga
     5101 catgaagctc cagaggctga ctgggctgga gcgtgacaag atcatcactg aatacaaaga
     5161 cacgttgaag gagatagaaa ggttaaaggc aatactggga agcgcggccc ttgtctcaaa
     5221 gatcatcaag gatgagctcc ttgagatcag gacgagatac gcggatgaga gaaaaacgga
     5281 gatcacggaa gagacaaagg agatcaccat cgaggatctc atcactgagg aggagatggt
     5341 gattaccctt tcccatcagc agtacatcaa gaggaatccc ctcagcgcat acaggagcca
     5401 gagacgcggc gggaagggtc tcatgggcat ggagacaaag gaagaggatt ttgtggagca
     5461 gctcttcatc ggttctaccc acgactatat gctcttcttc agcaacctgg gaaggctcta
     5521 ctggaagaag atctatgaga tcaaggagtt tggtcgcgct gcaaaaggcc aggcccttgt
     5581 gaatctcctt tccctcgctg aaggggaaag gattgccacc gcccttcctg tcagggattt
     5641 caaggagggg tacctgctca ccttcacgaa gaaaggcatg gtgaagaaga cggtcctcga
     5701 cgaatacagc aaccccaggg gcaaagggat tatcgctgtg acccttgagg agggtgatga
     5761 gctcatcact gtgaggaaga cggatggcaa gagtgatctc atcatcggca caagcgaggg
     5821 actctctata cggtttaacg aggaagatgt gagacccatg ggcaggagcg cgaagggggt
     5881 catcggcatt cgtcttcaaa aaggggatga agtcgtttcc gccgaggtgg ctgagatgaa
     5941 taccgccctt ctcacggtca ccgaaaaggg catcggaaag aggacgaagc ttgaggagta
     6001 ccgtgttcag ggaagagggg gcaggggggt aatctccata aagctcaccg aaaagggcgg
     6061 caaggcggtg ggcctcatgc aggtgaggga tgaggatgag gtggtgatga ttgccaacac
     6121 cggcaaactc atcaggactg ttgcagggaa catctccatc cacggaagga acacccaggg
     6181 ggtgaagctc atggatctgg atcctgaaga caggatcgtg agtataggaa gggtggcaga
     6241 gaaggattag ggtctaatct attctattca aaagtttcat tcttatagga gagatatccg
     6301 tctcctgaca agacaatagc ccttcttgac cagaccgggg tctgcgcatc taagaacatt
     6361 ctgttcccac gttttgtcag gtccgctcat gcggaccgat gcctaaccct tccgcctcag
     6421 gcggattgcc gttcgagccg tacacctctc ccgagtttat gaatatctgg gctaaaaagt
     6481 gcgaggtcgg gaaaactccg aaccttaacg aaaatctttg cagtatgccc cgtggcttgc
     6541 cacggggagt ttcacgtgcg cctggcatga cgcgcacact gaccttccgg cgaaggggtc
     6601 ggaagtaaag agtccgccgt taccggacgg aaggacaacg ccaacagggc aaggatatta
     6661 ccagaaatgg gatgtccgga ggaagcatga ggcagaagcc atgtacgtag cgaacagcga
     6721 accggctata tggcgttagg gagaaggtcg agcaggcgtg aaagggcaag acctgatatc
     6781 cggagaatcg aactgacgtg aagccggacg ggatatggtg aggtggcgtg tcttacccag
     6841 ggaagccctc ctgctctggc gttaggggtt gtcattaacg ctataaaacc gaaccgagga
     6901 ggagaacccg aggcggcaag ggtgcagagg gtggcagatt tacggatagt actaatgact
     6961 cagggccgat gaagcctggt aacagtgcgg aggagaaaac cctgacgatc agggagaggc
     7021 gaagcgaaaa gaacatgggg acagagaacc cgccatttat gacggtcaac acacaactga
     7081 cccagaagtc atggacattc gcagtcagac ctgaatgaac caatatcgtc gttgggacat
     7141 gggaagccgt gaacgaaaga cgacagtgac aactgtggcg agccgaatta gagttgagac
     7201 tgaagaccag aaaaccgaaa agggatagat cgacatcgaa agataccgag caggaaagaa
     7261 cctgtaaggt catgaggcta tcggtcaaaa gcggaaagcc agtccgaccc ggcaatcgtt
     7321 gacagaaagg agaagttact gaggagcctg atgaagcaag taggagtaat gggaagaatc
     7381 tatgagccgg gacgactgta caatgcgtgg cagcaagtaa agaagaacgc aggggcagcc
     7441 gggatagaca aaatgacggt aagggatttt gacagccgag agacagaact cttgggactt
     7501 atccatgaga agctgaagac cggcacatac cgtttcaaac cagcacggag agtgttgatt
     7561 ccaaaggaag ggacttccaa gatgaggaag ttaggaattc ctgcggtaat ggaccgaatc
     7621 gtgagccaga gtgtgaattc agtatttgca gagatattcg acccggattt tacggcatcc
     7681 aactttggat ttcgcaaagg caggagccag catatggcga taaggtatgt gcaggcaata
     7741 gtcagggaag ggtgtgaatg gtgcgcatca atagatctga aaagtttctt tgacgagatt
     7801 ccccacagcc ttatactgaa acttatccgc aggaagatag cggatgagag gctgttgacg
     7861 ctgattgcaa gagcgttaaa agccggagtg attgtggaag ggagattcga gaagacagag
     7921 aagggatgcc cacagggatc accactgtcc ccgatgatat ccaacattgt cctaaacgaa
     7981 ttggatcagg agcttgaaca tcgagggtta aagtacgctc ggtgggcaga tgactttgtg
     8041 atcttactta gatcagagag ggctgccaca agagtactag acggcatcac gaactatctg
     8101 aaagagaacc ttggacttcc agtgaatgaa gccaaaagtg gtgtcacaga aacaaagaag
     8161 gtgaccttct tgggctttca gattctggcg ggcaagattc gagtaagtga taaagctcgg
     8221 acaaagttca aggacaaaat cagggagttg actcgtagaa ataatccttt atcgatgtcc
     8281 cagttgatcg aagacatcaa cagatatatg caagggtgga tatcatactt cagcatacaa
     8341 gagttcaaat acttgttcag agattttgac gcatggatac gaagcagatt gagatcagta
     8401 caactgaaga aatggaagaa cccaaggaag tttcagcgta tcgccattaa atcgggatta
     8461 cggcctcagg aggctcggag aacatggctg cgaatgaaca aatggcaatc gacaaatagg
     8521 aaagaggttc gcttcgtgat gaacctcgcc tggttcagga agcttggttt ggtgttcctt
     8581 tatgattcta caccggcgac tttccaccca tagggtgatc gtcgatcgaa gagcggctta
     8641 cctggccggt acgagccgtt ccgttgggag ggggtggcca ttatgaccac ctcctaccag
     8701 attcaaccgt agccttttat agtttcccct ttgtgctggg aagccccttc actcttgggt
     8761 ctatggtgac cgcctcgcgg agcgcacgcc ccaggccctt aaaaatagcc tcgatgatgt
     8821 ggtggatatt ccttccgtag gggacattca catggagtgt gaccccgcca tggcttgtaa
     8881 atgcctggag gaagtcttcc acgaggtcgg cgtcaaaatc tttaattcgt gcttttttgg
     8941 gaagggtgac gttgtagaca agatacggtc ttccgctcat gtcgagactc agggacgcca
     9001 aagcctcatc catggccact gatgcatgac cgtatctcgt gatcccctcc atggttccca
     9061 gagactgttt gagcgccttg ccgagcacga tcccgatatc ttcgactgtg tggtgatagt
     9121 caacctcaat atctcccgtt gccctgaggc tcatgtcaag aagcccgtgc ttacacatga
     9181 gtgagagcat atggtcaaga aaagggaccg gactcttaat ggaatatttg ccccttccgt
     9241 cgaggttcag ctccatggat atggcggtct cccttgtctt tctctgaacc gtcgactttc
     9301 tcatgcaggc ctccttatgc aaagggatat ttgcatctcg ctcaattata ccttattgat
     9361 tcccaaaatg gcagagaggg gaaccattcg aggaaaaggc gaaggatgat gtataattga
     9421 acaaaacgca ttactcaata tcccatggaa tggagtgaag aatgaagaga ttgaaggtat
     9481 tccttttcac gatcatggtg gtcggcctcg ggagtttctc atttgccgaa ggagctgacg
     9541 cccagttcta ctttgatagg ggcagcagct ttctcaatga agggcagttc gataaggcta
     9601 tcaaggattt tagtgaggct ctttcccaaa acccaaaaat tacggaggcg tattataacc
     9661 gcggccaggc gtactataga aaggggcagt acgaaaacgc cattgccgat ttcaacaagg
     9721 tcatcgaggt atttccggat aacgccgata tctacaatct cagggggctc gcctatttca
     9781 aaacaggaca ataccagaat gccgtagatg acgagaccag ggctatctcc ctcaacccat
     9841 cctttgtggg tgccttgcat aatcgtggca ttgcctatgc aaggaccgga caatacgaca
     9901 aggccataga ggattacaat aaagccatta ccctcaaacc tgactatatg gacgcatact
     9961 actcgcgggg ggttgcgtat gtgagaaagg catcatccga tttcaccagg gcctgtgaaa
    10021 aggggaacaa atttgcatgc gaaaacctca agcagatttc gcagtaatag cctgcctgtc
    10081 tggcctgttg aaaatatcaa gtaaaaggag taggataaga gccatgtcgg ccctcatatc
    10141 ccgttttccc gttcacaccc tggaccatca gcaattactg cctgctggcg ctgaggtctc
    10201 gggggagacg ctggatgccc tcatctccac ccgcgtagcc gtttcctatg atagtctttc
    10261 cctgctccag cacgagtcgg taaaggagga tattctcacc tttctctgca cacccccctg
    10321 cgatatcata ttctccgacc ctcaggaggt aaccgagctc atgcatctca tggatacagt
    10381 ccatctcgcc ctcccctttc tgcagtcttt ggattatttc aaacagtatg acttctatac
    10441 ctaccgccat atactcatgg tctttgcctt ttccaccctc ctggcaaagg atctcctccc
    10501 cgactataaa gactggattc agcaggcggc aaccggccct acccatgact tcgggaagat
    10561 ctgcgttccc ctggagatac tgaaaaaaaa cacgcctctc accagagcag aacggagcat
    10621 catcgagcac cacaccactg cgggttatgt cctcctctgt tattatctcg gaggccccca
    10681 tcatcctgct gccagagtgg caagggacca tcacgaacgg agggatggct caggctatcc
    10741 ttgtggcgtt accctcatgg acctactggt ggaggtcatc tctgtggccg atgtctacga
    10801 cgcccttatt tctccaaggc cctaccgggt tgcttcctat gacaaccgga ccgctctgga
    10861 ggaaatcacc ctcctggcag aaaagaacaa aataagctgg gacgtggtaa gggcattggt
    10921 ggcgcataat cgcaaggaaa agccgcactt cagccagtgc gatgtctcgt acgagaaaag
    10981 gggcaagccc cccaaaggga atgtccatgg cattcttgct gaggattcgg aaaataagta
    11041 ggcagacacg ctctgtatga taccctgata tggtaagaag aagagagaaa gagggaatcg
    11101 catgaactat ctcatcatct atgcccatcc aaaccccgga agcttcaatc atgccatcat
    11161 ggaggccgtc acagaagagc tgaggcatca tggaaggggg gtgaagataa gggaccttta
    11221 ccagatggaa ttcaattcca tcctgaagtc agaggacttt gccaacatgc aaaggggttc
    11281 tgtggcccgg gatgtgaaag aagagcagga gttgattcgg tcagcagagg tgatgatttt
    11341 cgtctatcct ttatggtggg ccggtatgcc tgccatgctc aaggggtata tcgacagggt
    11401 ccttaccgag ggatttgcct acaggatcag tgatgaggga atcgcgggcc tcctcgccgg
    11461 gaaaaaggtt tttctcttca ccaccatggg tgcgtccatg ggggattacg aggagagcgg
    11521 ctttttcaag agcatgggcc agattacgga cacaggggta ttcgctttct gtggctgtga
    11581 agtcttaggc cacaagtatt tcccctcggt accctatgtc acggacagcg acagaaaaaa
    11641 gatgctggaa gaggtgagag agctagtaaa acaaaatctc ctgtgaaatg ctgttccccg
    11701 gagtgaagag acatgaggga tggacaagtc cgtaactgtc cacctccttt ttgaattcct
    11761 ccatcaggac atactcatcc gtgaagtgaa atttctggtg atagtcttcc gcagacgtaa
    11821 acccggggta gggcaggatt tgtgtcttta ttgcgacctt caatgtcgct gctacccgct
    11881 cctttgattc cgtacgggga aggcataatc gaaaaatggg gacacatccc atttttgact
    11941 ggttccatag aaaaaactgc cagagtgcag atgctcaaaa ccttagggaa aaacacacca
    12001 aaacataata acttgacaat cgtccacttt tatgataatt tatgttcaga aaataaccat
    12061 tgtttatctt caggcgatta tgggcacgat agaaagaaga gagcgcgaaa agcgggacat
    12121 gaagggtctg attttgaaaa cagcgatgag gctcttcttg gaagaaggct ttgagaaggt
    12181 cacgatccgt cggatcgccg aagagataga atatagccct gccaccattt acctttactt
    12241 cgctgacaaa gacgagatcc tttttgcgct ccatgagcag ggcttcgaga aactccacaa
    12301 actgcagcag acagtgctct ccatcaaaga tcccctggag cgtctgagaa ggcatggaaa
    12361 ggtgtacatc acgtttgccc ttaagcagcc cgaatactac gatctgatgt tcattatgag
    12421 gagcgctgct cggaaactta aggaggatga ggaatggact gcgggaagac gttcatacga
    12481 gttcctgagg gagaatgttg gaaaatgcat tgaagcggga tatctgccag gaggagattt
    12541 ggatatagca gcattctcgg tatggtcact ggttcatggc attgtctcgc tcattatacg
    12601 cgggcgctgc agcatgtttc cccaggagca tctggccgca atcgccgaag gagcactgga
    12661 atattcaatg agcaagatag ggactgggca gagctaaaaa tttttgccca tttattaaca
    12721 atgttcattt aataaacatg gtatttcaag ggggggtgaa ttatggaaaa gaagagtatt
    12781 ctggcaattt ctgggtccat gaggaaccca tcatttacgg aaaggatgct agacctctgt
    12841 gttgagggaa tgggagaggg cttcgaagta cgaaagtttt acccgcaccg gatgaaaatc
    12901 cgtccctgcc ttggctgctg gacgtgctgg aaaaaaacga gccctggcgt gtgcgtacag
    12961 aaggatgatt tcaaccaaat cctgagtgcc tataaagagg ccgactatgt tcttttagct
    13021 gcgccgctct atttttttgg tcttcccgct actgtcaaga acgtcatcga tcgcctcttt
    13081 gtgattcttg aaccagcgca atacaaatcg ctgcggggtg gcaccgagca tcccaagcgt
    13141 tacggccgcc atccgaaggc cgtgatcatt tcctcctgcg gttttcctga aatggagagc
    13201 tttgacctct tgaggcagca tttcaggaag atttgcactg aacttgaatg gaagtgggca
    13261 ggggaggtcc ttgtgcctgg cgcaggcact gcaaacgccc caaggctgtt tgacagaaaa
    13321 tacaggttaa tcaggcaggc tggtgaggaa cttgctacgg gggaaattag acctgatact
    13381 acagagagga ttgcttcttc agtcatgccg catgaggact accggaagat gtgcactgca
    13441 ggatttagag gcgacatatt aggaaagtta atttgcatcc tcattgccac gaaggcaatg
    13501 ttaaagcaag cgctttagtg aatccagacg aagcgcttca tcgctcgccc taaaatgaac
    13561 cggtctcccg ataagcccta ataaagagat aaagggcgga tgtaatttgt ggggaaatgc
    13621 ggcaaaagtc aggggctcta aaatcaaaac aggaatctca gcagataaaa ccctgcaatc
    13681 acggcaagaa gagagatgac tacagtcacg acgagaagcg tttttttgtc gtatggttcc
    13741 ttgcttgcgc gagcagccct atttgaacca tcacctttct ccggcacaaa ttcggacttc
    13801 tcgatctcct ctccggagga aatatggctg gtcattctta tgtcgagaag ttctgagatc
    13861 ttgaactcct tgtactgaat ctccatctct tttgggggta cggctccctt tctgcatttt
    13921 ttacattccg taccgtctgt tatctcttct ccgcaacgag gacactccat aaaatacttt
    13981 cctctcacac caataaaagt attataccag tttatcagcg tatctatcac aggtgctttg
    14041 taaatgggga cacatattat tttgtccata gccctgtgga tctacaggtg cgtaatccac
    14101 taggttcgca ttaaccaaaa tgagtgaaag aagccaacgc atatcattat gcagacaata
    14161 ctaaataaaa agccttataa tgtacccatg gccatagtgt tctttcgaag agattctcac
    14221 gggctagaag gaggtaggta tgtttgaaaa ctaccgcagg tatcagagat attcagtttc
    14281 agccaaggcg gtgataagaa ggttgattgc ggaatcacag aaaggtttca ctacgcgggt
    14341 aaacacaatc tcccaaggag gaatggggtt ttataccgat gtctttatgg agaaggccac
    14401 tccagtctct attgagttcc tcttcggtgc cctccaggtt agggaggaag acatccttgt
    14461 ggggaggatt gcctcggtct cttcccagga aaaggattat tttgtggggg tctcgtttga
    14521 gagggaaatc tcttatgacc ggtttatgga gatcgtcggc taagtgccaa aaacgcattc
    14581 cattttgcct aaaataggga cacatcccat ttgtcttgac gtttaaggtt ttcttctgta
    14641 gacttgactt atgccaagaa ttgcgagggc tgttgtacct ggattccccc atcacgttgt
    14701 ccagagaggc aacaataggg gagaaatatt ctttaatgac gaagatagag cagtgtacct
    14761 tttcctgctc aaaacata
//
LOCUS       DATKOU010000071         4866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_87581, whole genome shotgun sequence.
ACCESSION   DATKOU010000071 DATKOU010000000
VERSION     DATKOU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87581"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VMU21_03440"
     CDS             complement(<1..1295)
                     /locus_tag="VMU21_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HUO76609.1"
                     /translation="MVKFTCLQGKASQGFFSDGERPCKNQIAEKMTKEDGIKNFILRT
                     NSKFFLSCFLVNILLFVGLLTTDRLYIDDIGRSVSGESGWSGVGRPLADMFITFIQLG
                     TPLNNSFPLPQIISICMISLTCMLLSQTYSIKSPIIAAICTLPLAGQPYYLENMSYSF
                     DSPSMALGLSLSVCAAMSLADKKREGFIISVVLAFMALNLYQPSIGAFFVTYIFLVIK
                     NINIEIKPHKYFLAHITRLITGSGLAFLLYVPVVRKFAVADYAIEHSKLIGLHNAYDK
                     IFGNTANYIGALIRDWSGNTIGYIFVFIFVSFLVVSIFQAIWSGHRFSINKRTFLYAV
                     LSLVFGVLTVIVSYFPQMLLENPLLFPRVFVGFGAILSCCCLYVWSTLEANNFGLGKY
                     EQYIKAIPVCLVAYALITFSYSYSRANAAQRQYEDIVIGG"
     gene            complement(1202..1921)
                     /locus_tag="VMU21_03445"
     CDS             complement(1202..1921)
                     /locus_tag="VMU21_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HUO76610.1"
                     /translation="MGQRKEFISIIIPAYNESARILPSLRRLDEYLHGHFRGFELIVV
                     NDGSSDTTEDVVVHSKKEIPSIQYSGYKENRGKGYAIRQGVALSTGDIILVSDADLST
                     PIEEVEKLLVPYDDGYHVVIGSRGLEASSIMVRQPWWREFMGKTFNRIVRMLLLEGFK
                     DTQCGFKLFNGSIGRELFKQATVDRFAYDVEVLYLAVKAGCKIKEVPVKWLNSPASRV
                     RPLRDSFQMAKDLVRIRLRKK"
     gene            complement(2085..4418)
                     /locus_tag="VMU21_03450"
     CDS             complement(2085..4418)
                     /locus_tag="VMU21_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HUO76611.1"
                     /translation="MFFFILFLSGIVFFYVFLYFPLTAGIVSILSAARLISRGRVLLV
                     IPFILGGSYAWFRYSPPLDPSIISREIRIQCVVKDSPHELSSGRLLNEVTIQSAVDAD
                     KGEPLEFLKDGEMSIISDNGLKRGLRYGLTVRLVGDKERRNPGSMRNDALYAYLQEVS
                     SAEPFKENAPILWFRDRRDMLTQYLKGNFQGDSAALLASITTGERSTMSEELKDAFNA
                     TGLVHLLSISGTHFGLFSMLIFGMFRILVRYLPYRILQRLTIYLTPPQVAALASLPFM
                     LMYLFISGSSIPALRSFIMINVFLLGLLIGRKGFWLNSLLFAAFLVCVWDPAAILTIS
                     FQLSFLAVFFIGYAFGEREEKPVKEGVPARVTGYLKNSLILTLAASLGTAPLVAYYFH
                     YFSVVSPLANLLITPFIGFVLVALSLIAAFSYIFTGYYPFQSLVALATDISIKGVKLF
                     ASVPFADIRISAFPAVVIIVFYAGTLVCFLSGRKRYLLALPLVTIMICSAFLIKGKDA
                     ISVTYLDVGQGDSAVVEAQGGKIMVIDTGRTGREVVAYLRYLGQRNIDVLMVTHADDD
                     HAAGVPYVIKRSVVKEVWDNGLLIYPDDFLRTETHRSLERGDTTKTYGLDIQALHPYK
                     GFFTFSDNEAAAENNDSLVVKITGRNKSFLFTGDIAEEAEEDLVHLGKWLKSDVLKVS
                     HHGSRTSSSEDFLYTAAPEIGVISVGRDNTYGHPHKETLERLQGIKVYRTDREGAVKV
                     TETPGGLAVKTYRDFPLERTRSVGGELRNIKRLFIEW"
     gene            complement(4418..>4866)
                     /locus_tag="VMU21_03455"
     CDS             complement(4418..>4866)
                     /locus_tag="VMU21_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HUO76612.1"
                     /translation="EEMLRGGYSLGGEPSGHVISLDCNTTGDGPITALHVLYAMKKEN
                     ASLSDLVADIRLYPQVLINVPLQDKEHPKTSKTPRGGRTDFKSHPVIVQAIRDAEDRL
                     KGRGRLLVRPSGTEHKIRVMVEADEKRLADTLARRIAEAVKKTMSV"
BASE COUNT         1329 a         1204 c         1117 g         1216 t
ORIGIN      
        1 cccccaatga caatatcttc atactgcctt tgcgccgcgt tggccctgga atatgaatag
       61 gaaaaagtta tgagcgcata tgcaactaga caaactggta tcgcctttat atactgttcg
      121 tatttcccca agccaaagtt attagcctcc aaggttgacc agacatataa acaacagcag
      181 cttaatatcg cgccaaatcc aacaaacact cttgggaaca agagaggatt ttcgagaagc
      241 atctgtggga agtaggaaac aatcacggtg agaaccccaa aaactaaaga caaaaccgca
      301 tagagaaagg ttctcttatt tatggaaaaa cgatgaccgg accatattgc ctgaaatatt
      361 gatacaacta aaaaagaaac aaatatgaac acaaaaatat accctatagt attcccggac
      421 cagtccctga ttagtgcgcc gatgtaattg gcagtgttgc cgaatatctt atcgtatgca
      481 ttgtgcaacc caatcaactt actatgttca atggcatagt cagccactgc gaacttacgc
      541 acaacaggaa catacaataa aaaagcaaga cctgagcctg ttattaatcg ggttatatga
      601 gcaagaaaat atttgtgggg tttgatttct atattgatat tttttatcac caaaaaaatg
      661 tatgtcacga aaaaagcccc tatggaaggt tgatataaat ttaatgccat gaatgccagt
      721 acgacagaga tgatgaaacc ctcccttttt ttatcagcca aactcattgc ggcgcatacc
      781 gaaagcgata accctaatgc catcgaagga gagtcaaaac tgtacgacat gttttcaagg
      841 taatacggct ggccagctag ggggagcgtg cagatagctg cgataattgg cgacttgatt
      901 gaatatgtct gtgacagcaa catgcatgtc agagagatca tacatattga aataatctgg
      961 ggcaaaggga aagagttatt taaaggagtt cctaactgta tgaacgtaat aaacatatcg
     1021 gctagtggcc ttccaactcc tgaccatcct gattcgccgg acacagagcg tccgatatca
     1081 tcaatataaa gacggtcagt agtgagaaga ccaacaaaaa gaagaatatt gactaggaaa
     1141 catgacaaaa aaaactttga attggttctc aatatgaagt ttttaatccc atcttctttt
     1201 gtcatttttt ccgcaatctg attcttacaa ggtctttcgc catctgaaaa gaatccctga
     1261 gaggccttac cctggaggca ggtgaattta accattttac cggaacttct tttattttac
     1321 agccggcctt gaccgcaaga taaaggacct cgacatcgta tgcgaatcga tcaacggttg
     1381 cctgtttaaa tagttccctg cctatactgc cattaaacag cttaaatccg cactgggtat
     1441 ctttgaatcc ttccaagaga agcatcctga caatcctgtt gaatgtcttg cccataaact
     1501 ctcgccacca gggttgtcgt accatgatac tgcttgcttc caatcccctg gacccgatca
     1561 ccacgtgata gccatcatca tacggaacaa gaagcttctc cacctcttct atcggagttg
     1621 aaaggtcagc atctgaaacg aggattatgt cgcccgtgga gagtgcaacc ccttgcctga
     1681 tcgcatatcc cttgcctctg ttctctttat accctgagta ttgtatggat ggaatttcct
     1741 ttttggagtg cactacgaca tcttcagtag tatccgagct cccatcattc accacgataa
     1801 gttcaaaacc cctaaaatgg ccatgcaaat attcgtccag ccttctgagg gaagggagga
     1861 tacgcgcgga ctcattgtaa gcggggatga tgatagagat gaactctttt ctctggccca
     1921 tggccttcct atttttccgt tatcttatat atacatagca caatctttca tgggatttcc
     1981 aatatattcc ctggttgttg acaccttttc cggcttaaac aatttcgggt tgactctatg
     2041 ggtatggcat cctgaccagc gtcaattatt tgatgacctg tttcttacca ctctataaac
     2101 agccttttta tgttcctcaa ttcgcccccg acacttcttg tccgttccaa agggaaatcc
     2161 ctgtaagtct tcacggcaag ccctccgggt gtttcggtca ccttcaccgc accctcccgg
     2221 tccgtccgat agacctttat cccctgaagt ctctccaggg tttccttgtg cgggtgaccg
     2281 taggtgttgt cccttcccac acttatgacc cctatctccg gggccgccgt atacaagaaa
     2341 tcctctgatg aggatgtccg gctgccgtga tgggagacct tgagcacatc actcttcagc
     2401 cacttcccga ggtgcaccag gtcctcctct gcctcttcgg caatatcacc ggtaaagaga
     2461 aacgacttgt tcctcccggt gatttttacc acgagggagt cattgttttc ggcagcagcc
     2521 tcattgtctg aaaaggtaaa aaaccctttg tagggatgaa gggcctggat atcgagtcca
     2581 tacgtctttg tcgtgtctcc cctttcaagg gaacggtggg tctctgttct taaaaaatca
     2641 tccggataga tgaggagccc attgtcccat acctccttca ccacagacct ctttatcaca
     2701 tagggcaccc ctgccgcgtg atcgtcatcc gcatgggtga ccataaggac gtcaatgttc
     2761 ctttgcccaa gataccggag ataagcgacc acttccctgc ctgtcctccc ggtatcaatg
     2821 accattatct ttcccccctg ggcctcgacc accgcagagt cgccctgtcc cacatcgaga
     2881 taggtgaccg agatagcatc tttccccttt atcaggaatg cggaacatat cattattgtt
     2941 acaaggggta gtgcaagcag gtatcgcttt ctgccggaca gaaaacaaac cagggtccct
     3001 gcatagaaga ctatgattac cacggcagga aacgcggata tccttatgtc tgcaaagggg
     3061 acggaggcga ataacttgac cccttttatg gaaatatcgg ttgcaagggc aacgagggac
     3121 tgaaaagggt aataccccgt aaatatgtag ctaaaggccg cgatgaggga aagagccacc
     3181 aggacaaatc cgataaaagg ggttatgagg aggttcgcaa gaggcgagac gacggaaaag
     3241 taatggaaat agtacgctac aaggggggct gtcccaagtg atgctgcgag cgtgaggatg
     3301 agggagttct tcagataacc cgtaacgcgg gcgggaactc cctctttcac gggtttttct
     3361 tctctctctc caaaggcata ccctatgaag aacaccgcaa gaaatgagag ctggaatgat
     3421 atggttaaga tggctgccgg gtcccacacg cagacgagga atgcagcaaa taaaagggaa
     3481 ttgagccaga accccttcct ccctatgaga agtcccagga ggaatacatt aatcatgata
     3541 aatgacctca atgcgggaat acttgagccg gaaatgaaaa gatacatgag catgaatggc
     3601 agagaggcaa gtgccgcaac ctgtggaggc gtgagataaa tggtgagtct ctggagtatc
     3661 cggtacggca gatagcgaac gagaatcctg aacattccga agatgagcat tgagaaaagt
     3721 ccgaaatgtg tgccggaaat gctcagcaaa tggacaaggc ctgtggcatt aaaggcgtcc
     3781 ttcagctcct cgctcatggt tgacctctcc cctgtagtaa tggaagcgag cagggcagcg
     3841 gaatcgccct gaaagttccc tttgaggtat tgggtgagca tatccctcct gtctctgaac
     3901 cataagatgg gagcattctc tttgaagggt tccgcgctgc taacctcctg gagataggca
     3961 tagagcgcat cattccgcat actgccgggg ttacgtcttt ccttgtcccc gaccagtctc
     4021 accgttaatc catacctcag cccccgtttc aacccattgt cagagatgat gctcatctcc
     4081 ccgtctttga gaaactcaag gggttcccct ttgtccgcat caaccgccga ttggatagtg
     4141 acctcattca ggagccttcc tgaagagagc tcgtggggtg aatcctttac cacgcactgg
     4201 atgcgtatct ctctacttat tatagagggg tccagaggag gggaatacct gaaccacgcg
     4261 tatgatcccc caaggatgaa cggaatgaca agaaggacac ggcccctgga aatcagacgg
     4321 gccgccgaga gaatggatac tattcccgca gtaaggggga agtagagaaa cacataaaaa
     4381 aagacaatgc ctgagaggaa cagaataaaa aagaacatca gacggacatg gtcttcttta
     4441 ccgcttctgc gatcctgcgg gcaagcgtat cagcgagcct cttttcgtca gcctccacca
     4501 tcacccttat cttgtgttct gttcccgagg gcctcacgag aagccttccc ctgcctttca
     4561 ggcgatcctc tgcgtccctg atcgcctgca caatcacggg atgtgatttg aagtcggtcc
     4621 tgccgcccct cggtgtctta ctggtttttg ggtgttcctt gtcctgcagg ggtacgttga
     4681 tcaagacttg aggataaagc ctgatatccg caacaagatc tgacagagag gcattttcct
     4741 ttttcatcgc atagaggaca tggagtgcgg taatgggacc gtcccctgtg gtgttgcaat
     4801 caagggatat gacatgtcct gatggttcgc cgccgagact gtaaccaccc cggagcattt
     4861 cctcgg
//
LOCUS       DATKOU010000072         4916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5091691, whole genome shotgun sequence.
ACCESSION   DATKOU010000072 DATKOU010000000
VERSION     DATKOU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5091691"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VMU21_03460"
     CDS             <1..1123
                     /locus_tag="VMU21_03460"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HUO76613.1"
                     /translation="IETGLTFVGLVGMMDPPREEAMEAVALCKKAGIRPVMITGDHPL
                     TAEVIAKRLGILEHDSRAIMSGRTLEILSPEELGKQVEDIRVYARVAPQQKLKIVKAL
                     QDRGQFVAMTGDGVNDAPALKRADIGIAMGITGSDVAKEAAHMVLLDDNFATIVKAVK
                     EGRKIYDNIRKFVRYLLTTNSGELWTLFLAPFAGLPMPLLPIHILWINLMTDGLPALA
                     LSMEPPEGDVMQRPPRNPRESIFAHGLWIHAIWVGLLMAGIVLLLQAWTITTGSIHWQ
                     TMVFTVLCLTQLGNVLAIRSERESLFTIGLFSNKPLLGAVVVSFLLQMAVVYMPWLNP
                     VFRTEPLSPGELALALVSSSLVFFAVETEKAWRRARSAR"
     gene            1113..1667
                     /locus_tag="VMU21_03465"
     CDS             1113..1667
                     /locus_tag="VMU21_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUO76614.1"
                     /translation="MHVNPLDVIQKYYDPGSKAYYFLVLHGRLVAKKACELGRTVAHL
                     NPDMKFIEEASLLHDIGMFLSNAPKLGCYGHKEYVCHGYLGRELLDREGLPRHALVCE
                     RHVGIGITLEEIKTNSLPLPPREMVPVSVEEKIVCFADKFYSKSEHDLLREKPLERVR
                     KMIRAYGEDKLKIFDEWSQLFTLS"
     gene            complement(1701..2276)
                     /locus_tag="VMU21_03470"
     CDS             complement(1701..2276)
                     /locus_tag="VMU21_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76615.1"
                     /translation="MDAYRYQQLAYLIVPVLLGIEFFISAKDEKRQREDTPLGSYVLD
                     FLGFLFMALVPAIFIFTIWAVETRSFPLGGLALARFDRYAVLFLFMGSWWQVHMIAAL
                     RARRMRQREGTHWYLWGPYLVLGIFVSVLILWVSPFHLKWVSMIWFFLVFGMLRIARA
                     TPKTVERLLWIFVAVFFMLENIIFMFLETVV"
     gene            complement(2313..3050)
                     /gene="larB"
                     /locus_tag="VMU21_03475"
     CDS             complement(2313..3050)
                     /gene="larB"
                     /locus_tag="VMU21_03475"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HUO76616.1"
                     /translation="MNRAQVLALIQSIKNGSLSVEKALEKLTHLPYEDLSFAKIDHHR
                     HLRQGIPEVILSRGKTVPEIRAIAKSMYKRSKRFLITKATRSVHQELGIKKASFYPAS
                     GIIEVGAEVKKKEGLILVLSAGTSDIPAAEEAAVTASFLGSNVESVYDVGVAGIHRLL
                     DKRRIFSLARVIVVVAGMEGALPSVVGGLTDRPIIAVPTSTGYGTSLGGLTALFAMLN
                     SCVPGIAVMNIDNGFGAGCLAHKINTV"
     gene            complement(3043..4038)
                     /locus_tag="VMU21_03480"
     CDS             complement(3043..4038)
                     /locus_tag="VMU21_03480"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HUO76617.1"
                     /translation="MSYIAVIGAGSWGTALSRLLAEKEFDVSLWVYENDLCGEMRRTR
                     INSPYLPGFPIPDNIVITDDLKEAVDKARYILNVVPTQYARTIMKNALPHISPDSVII
                     NASKGIEQKSLLTFSAIMKELTHHRVAVLSGPSFAQEVAKKLPTAVTLSTDDAPTALL
                     LQEIFNTHYFRVYAHNDILGVELGGALKNVIAIASGISDGLALGHNARAALLTRGLAE
                     IVRLGVAMGAREQTFSGLSGLGDLILTCTAVLSRNYAFGTKLAQGMKVNDILSQTKSV
                     VEGVATCESAYELSRKYRVQMPFVEQIYMVLYREKDPGLAVTELMNRALKTEFHE"
     gene            complement(4109..4342)
                     /locus_tag="VMU21_03485"
     CDS             complement(4109..4342)
                     /locus_tag="VMU21_03485"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HUO76618.1"
                     /translation="MSEEWVVILVTYDSLEAEMIKDLLESGGMPVVLRSSKVSPYPVN
                     IGKMGEIKVLVRKEDQDLAEEVIRGTGSNGTEE"
     gene            4508..>4916
                     /locus_tag="VMU21_03490"
     CDS             4508..>4916
                     /locus_tag="VMU21_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76619.1"
                     /translation="MVRKIHKRISARPFGQMIIIVFALSFFLCSTLSADSFWDTLDKS
                     KIQKEIVLTNETVVKLVERLKPAVVNISVTQLVKQEIPQGFGAEDPFGDFWRRFFGNQ
                     EPKEFKRKGLGSGFIINKDGYIVTNNHVVQKAKD"
BASE COUNT         1264 a         1247 c         1190 g         1215 t
ORIGIN      
        1 tatcgaaaca ggccttacgt ttgttggtct cgtagggatg atggaccccc cgagggaaga
       61 ggcaatggag gcagtagcat tgtgcaaaaa ggcaggaata cggccggtga tgataacagg
      121 cgaccatcct ctcaccgcag aagtgatcgc gaagaggctc ggcattctcg aacatgactc
      181 cagggcgata atgtcaggga gaacgcttga gatattgtca ccagaggaac tcggaaaaca
      241 ggttgaagat atcagggtat acgcaagggt cgcgcctcaa cagaaactaa aaatagtgaa
      301 ggctctccag gacaggggtc aattcgttgc aatgaccggg gatggcgtta atgacgcccc
      361 tgcgctgaaa agagctgaca ttggcatcgc gatgggcatt acggggtcgg atgtggcaaa
      421 ggaggcagct cacatggtcc tccttgatga taactttgcc acaattgtaa aggccgtaaa
      481 agagggaaga aagatctacg acaatataag aaagttcgtg agataccttc tcaccaccaa
      541 ttcgggcgaa ttatggacgc tctttcttgc accctttgca ggcctgccca tgccccttct
      601 gcctatccat atactctgga taaaccttat gaccgacgga cttccggccc ttgccctctc
      661 catggaacct ccggagggcg atgtgatgca aaggccgccg cgcaatccgc gggagagcat
      721 atttgcccat ggcctctgga tccatgcaat ctgggtaggt ttgctcatgg caggcatcgt
      781 ccttttactt caggcttgga cgatcaccac cgggagtatc cactggcaga cgatggtctt
      841 cactgtcctc tgtcttaccc agctgggaaa cgtactggcc atccgctctg aaagagagtc
      901 cctcttcacc attggcctgt tctccaacaa gcccctgctc ggtgctgtag tggtgagttt
      961 tcttcttcag atggctgtag tctacatgcc ctggctcaat cccgtgttca ggactgagcc
     1021 cctttcaccc ggtgaattag ccttggccct tgtctcatca tctctggtat tctttgcagt
     1081 ggaaacggaa aaagcgtgga ggagagctcg gagtgcacgt taatcctctt gatgtgattc
     1141 aaaaatatta cgacccaggg tcaaaggcat actattttct ggtacttcac ggcagacttg
     1201 tggcaaaaaa ggcctgcgaa ctcggccgga cagtggcaca tctcaatcct gatatgaagt
     1261 ttatcgaaga ggcttccctg cttcatgata tagggatgtt cttaagtaat gcaccaaagc
     1321 tgggatgcta cggacataag gagtatgtat gccatgggta tctcgggaga gagttgctgg
     1381 atagggaagg gcttcccaga cacgcactcg tttgtgagag gcatgtgggt atcggcatta
     1441 ccctcgaaga gataaaaacg aatagtcttc ccttgcctcc aagggaaatg gtccccgttt
     1501 ccgtggaaga gaagattgtc tgttttgcgg acaaattcta ctcaaaaagc gagcatgatc
     1561 tcctccgtga aaaaccatta gaacgcgtaa gaaaaatgat acgggcatat ggagaggata
     1621 aattgaagat ttttgatgaa tggtcacaac tgtttaccct gtcgtgacat ggtcgctctc
     1681 cggcttaaac ctttttttcg ctacaccacc gtttcaagaa acataaagat gatattctcc
     1741 agcatgaaga agacggcgac gaagatccag agcagacgct caacggtctt tggcgttgcc
     1801 ctggcgatgc ggagcatacc aaacacgaga aagaaccata tcatggaaac ccacttcaga
     1861 tgaaagggag atacccataa tatcagcacg gatacaaaga tgccgaggac aagatacggt
     1921 ccccacagat accaatgagt tccctccctt tgcctcattc tcctcgctct cagtgcagca
     1981 atcatgtgca cctgccacca tgatcccatg aaaagaaaga ggaccgcata acgatcgaac
     2041 ctcgcaaggg caagcccgcc aagggggaat gaccttgtct ccacagccca aatggtaaag
     2101 ataaatatgg caggaaccaa tgccatgaac aaaaaaccaa gaaagtcgag gacatatgag
     2161 cccaggggtg tgtcttccct ttgtctcttc tcatcttttg cggaaatgaa aaactcaatc
     2221 ccaaggagca cagggacgat aagataggca agctgctgat acctgtatgc gtccatctta
     2281 cctcccccaa gcgcctcttt atttttttgt acttaaaccg tattgatctt atgtgcgaga
     2341 catcctgcgc caaagccatt gtcaatattc atcaccgcga tccccggaac acacgaattg
     2401 agcatggcaa aaagagcagt caatccgcca aggctggttc catatcctgt tgaggtggga
     2461 actgcgatga ttggcctgtc ggtcagtccc cccaccacag aggggagggc cccttccatt
     2521 ccggctacca ccacgataac ccttgcgaga gagaagatcc tccttttatc aaggagcctg
     2581 tgaatccccg caactcccac atcgtagacc gattccacat tgctccccaa aaacgatgcg
     2641 gtgactgcag cctcctcggc tgcagggatg tcggatgtgc ctgcagaaag gaccaggata
     2701 agaccttcct tcttcttcac ctctgcgccc acctcgatta ttcctgaggc aggataaaat
     2761 gacgccttct taattcccag ttcctgatgg actgacctcg tcgcttttgt gatgaggaac
     2821 cttttgctcc tcttatacat tgactttgcg atagctctga tttcagggac cgtctttccg
     2881 cgggagagga ttacctcggg gattccttgc ctgagatgcc tgtggtgatc gatctttgca
     2941 aatgaaaggt cttcataggg gagatgtgtc aacttttcta atgccttttc tacagaaaga
     3001 gagccatttt ttatggattg tataagtgcg aggacctgtg ccctattcat ggaattctgt
     3061 ctttagcgca cggttcatca attcagtcac cgcaaggccg ggatcctttt ccctgtaaag
     3121 aaccatatat atctgctcaa caaagggcat ctggactctg tacttcctcg aaagctcgta
     3181 tgctgactca caggtcgcca ccccttccac gacgctcttc gtttgcgaaa ggatatcgtt
     3241 gaccttcatc ccttgggcca gcttcgttcc aaaagcgtag tttctcgaca ggactgcggt
     3301 gcatgtgagg atgaggtctc ccagaccgct caggcccgag aatgtctgtt cccttgcacc
     3361 catcgcaacc cccagcctca ctatttcagc cagtcccctg gtgaggagcg cagccctcgc
     3421 attatggccg agtgcaagac catcagagat tcccgaggca atggcaatta cattttttaa
     3481 ggcaccccct agctcgactc ccaaaatgtc attatgggca tacaccctaa agtaatgggt
     3541 attgaaaatt tcctgtaaga ggagtgcagt aggtgcatca tcagtggaca aggttactgc
     3601 agtaggaagt ttctttgcca cctcctgggc aaagcttgga cccgagagca ccgcaaccct
     3661 gtggtgggtc agttccttca ttatggcgga aaaggtgagg aggctctttt gctctatccc
     3721 cttcgaggca ttgatgatca cagaatcagg gctgatgtgg gggagtgcat ttttcatgat
     3781 cgttctggcg tattgtgtgg gcaccacatt caggatatag cgtgccttat ccaccgcctc
     3841 tttaagatca tcggtgatca cgatattatc cgggatgggg aagccgggaa gataggggga
     3901 attaattctg gttcttctca tctccccgca gaggtcattc tcatagaccc agagggaaac
     3961 gtcaaactct ttttcagcaa ggagacggga aagggctgtc ccccaacttc ctgcgcctat
     4021 gactgcaata tagctcatgt atcggtgaat gtgaaatgaa tataacagat tgtttgccag
     4081 cgatgcaatg aggggcaaag ccctcttctc attcctccgt gccgttgctt cccgtccccc
     4141 tgatcacctc ctctgccaag tcctggtctt ccttccttac aagcacttta atctcgccca
     4201 tcttcccgat gttcacaggg taggggctca cctttgaaga cctgagcact acgggcatcc
     4261 ctccgctttc cagaaggtcc tttatcatct ctgcttcaag ggaatcatac gtaactaaga
     4321 taaccaccca ctcttcactc atttctcttc acagacctcc aagcctgctc tcatgctcac
     4381 attgtaacgg acaatgcgac tgaatgaaag atggtccagc cttttttgca tcttccgcca
     4441 ctctggagta taatacaaca aaattgcacc atacattatg acgcgaaaac ctttgaggag
     4501 gcattgaatg gtcaggaaaa tacacaagag aatttctgca cgaccctttg gtcagatgat
     4561 aataatcgtc tttgcgctat ctttttttct gtgcagtaca ctctcggcag attccttctg
     4621 ggatactctg gacaaatcaa agattcagaa agagatcgtc cttactaatg agacggttgt
     4681 aaaactggtg gaaaggctta aacctgcagt ggtgaatata agcgtaacac agcttgtcaa
     4741 gcaggagata ccccagggat ttggagctga ggacccgttt ggagatttct ggagaaggtt
     4801 ctttggcaat caggaaccga aggaattcaa gaggaaaggc ctcggctccg ggtttattat
     4861 taataaggac gggtacatcg ttaccaataa ccatgtggtg cagaaggcaa aggata
//
LOCUS       DATKOU010000073         4491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4325723, whole genome shotgun sequence.
ACCESSION   DATKOU010000073 DATKOU010000000
VERSION     DATKOU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4325723"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VMU21_03495"
     CDS             complement(<1..859)
                     /locus_tag="VMU21_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HUO76620.1"
                     /translation="MSLRKLLSIRNTLAFRLTLWYAGIFTVSSFIAFLLFYALITSVI
                     RERTDQELLSQVNRFSVLLTNEGVEAVENGTLIEAQAAGVKKVFFRLLQLNGQVYSST
                     NVSYWQDIGIDRVALQALLRGDAPVFKTIVIPNRRDKVRILYAMLSPSMILQVGQAME
                     SYSRFLDAFKGIFIGTMSFLIVIAAGIGWFMARRAVSGVEAVTRTARKISGGILQERV
                     PVKGSGDEIDQLALTFNEMLDRIQILLAEIKEMSDNIAHDLRSPITRIRGGAEVTLST
                     GKSLSEFESM"
     gene            complement(837..1514)
                     /locus_tag="VMU21_03500"
     CDS             complement(837..1514)
                     /locus_tag="VMU21_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO76621.1"
                     /translation="MRILLVEDDAKIASFIVKGLRAAGYAVDHVPDGENGLHLALTEP
                     YDAAIIDIMLPKLDGLSLIKKMRTESVHTPVIILSAKGSVDDRVKGLQTGSDDYITKP
                     FAFSELLARVQALIRRGSGQVEPTRLSVGDLSMNLITREVTREGRKIELQPMEFSLLE
                     YLMRNAGKVVSKTMIMEHVWDYNFDPQTNVVEARISRLRDKIDRDFSRKLIHTIRGVG
                     YVVKETS"
     gene            complement(1563..2633)
                     /locus_tag="VMU21_03505"
     CDS             complement(1563..2633)
                     /locus_tag="VMU21_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUO76622.1"
                     /translation="MTKDYSRRDFFKGLGLGTIGLGFGVSVFDGIYQYAEATTEEEKH
                     ALLMKGSVNFMGFMAKEITPNSQFYITTYSDTVPTIDPNAFRLRIEGLVEKPYTLSLK
                     ELEEMKDKAEFVTLECIGNPIGGDSISNALWDGVTLKSIIDRAVPKKGIVKTAFYADD
                     GYSDSIPYSLSLSEDVFLAYRMNGEALPKEHGYPLRVIVPGIYGMKHVKWLSKIELVN
                     YNFKGYWEKKGWSDDAVIPAKSQILMPMDGTTVPIGNYVIGGVAFGGRYGISKVQISL
                     DDKRTWHDTELKTPLSKWSWTLWRYDWKPSKEGEYTITVRGFDRTGKVQESSSLLGKL
                     FRSFPDGAKGLHSVKVKAGKAG"
     gene            complement(2755..3669)
                     /locus_tag="VMU21_03510"
     CDS             complement(2755..3669)
                     /locus_tag="VMU21_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76623.1"
                     /translation="MNRTKKILAMAFTMFIGGLFLMGEGQAFAEMSGMQGHQHEGMQH
                     MHGGGGSEVIVGIKTEPERIIVGTLTTVVFSFKNREGKPVENLTIHHDRLIHVVISSK
                     DFSVFAHIHPQDFGLITPEMKKTALYPVRFTFPKAGRYIIGIDFAVNDQPLSRHFLVD
                     VAGEPKMGPPKQDFSREKRFGDLDVTFASTPERIMSGKEVTLSYIFKKNGKPVTDLEP
                     WLSAPMHLAIISVDLTQFIHTHGELPGMPAMGHHEHHMQMMMTVPEKFGPKIEVHMIF
                     PAAGLYQIFGQVGYQGKVIATSFMVRVD"
     gene            complement(3747..>4491)
                     /locus_tag="VMU21_03515"
     CDS             complement(3747..>4491)
                     /locus_tag="VMU21_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HUO76624.1"
                     /translation="GDGVNDAPALKQAEMGIAVSNATDVAKASASVVLTEPGISVIIN
                     AIIISRQTYQRMLTWVINKVTKVIEFVGLLTLSFFWFHDIVLSLLGMSLLVFANDFVT
                     MSLATDNVKHTPNPNMWNVKNITLASLVPGMLLVVEGILVILMGMNYFHLGWEKLRTL
                     VMLNLIFNSQFRVLIVRERRHFWSSMPGRELFVLSAGTIIGFTLLGIYGVFVPSLTLY
                     QVLTVLIFSPVFTLGIDFPKFYVFRRFGL"
BASE COUNT         1153 a         1197 c          959 g         1182 t
ORIGIN      
        1 ccatactctc aaattcactc agggatttgc ctgtgctgag ggtcacttcc gcgcctccac
       61 ggattctggt gatggggctt cttagatcat gggcgatgtt gtcgctcatc tctttgattt
      121 ccgcaaggag gatttgaata cggtcaagca tttcattgaa tgtgagtgcg agctggtcga
      181 tttcatcgcc gctgcctttt accggaaccc tctcctgaag gatacctccc gatatcttac
      241 gggctgtacg agtgactgcc tcgactccgg aaacagcccg tctcgccatg aaccagccta
      301 tcccagcggc gatgacgatc aggaagctca tggtgccgat aaatatcccc ttaaatgcgt
      361 cgagaaaacg ggaataactc tccatggcct gtccgacctg caaaatcatg gacgggctaa
      421 gcattgcgta aaggattcgc actttgtccc tgcggtttgg aataacaatc gtcttaaata
      481 caggggcatc accccgaagc agcgcctgta gggcaaccct gtcaatgccg atatcctgcc
      541 agtaagaaac gttggtcgag gagtagacct gcccgtttaa ctggaggagg cgaaagaata
      601 ctttttttac tccggctgcc tgggcctcaa tcagcgtgcc gttttctacc gcctcgaccc
      661 cctcattcgt gaggaggacc gaaaacctgt tcacctggct cagcagttcc tggtcggtac
      721 gttcacggat gactgaagtt atgagagcat aaaacagaag aaaggcaata aaggaagata
      781 cagtaaatat cccggcatac cagagggtta gccggaaagc aagggtattt cggatgctaa
      841 gaagtttcct taacgacata tcctacgccc cgaatggtat ggatcagctt ccttgagaaa
      901 tctctgtcga tcttgtccct tagcctagaa atcctcgctt ctactacatt agtctggggg
      961 tcgaaattgt aatcccacac atgttccatg atcatggttt ttgaaaccac tttccctgca
     1021 ttccgcataa gatactcaag aagggaaaac tccataggct gcaattcgat ctttctgccc
     1081 tctctggtca cttcacgtgt aataagattc atggagagat ccccaacact caaccgtgtt
     1141 ggttcaactt ggccgctccc tcttcggatc agtgcctgta cccgggcaag aagttcagaa
     1201 aaggcaaagg gttttgtaat gtagtcgtcg cttccggtct ggagtccctt gacacggtcg
     1261 tcaacagacc ctttggcgct caggatgatg acgggcgtat ggacactttc tgttcgcatt
     1321 tttttgatga gtgacaaacc gtccagtttt ggcaacatga tatcgatgat ggctgcgtca
     1381 tagggttcgg taagtgccaa atgaaggccg ttctcaccat ccggcacatg gtctaccgca
     1441 taccccgcag cccgtaaccc tttcacgatg aaagaggcta tcttggcatc atcttcaacc
     1501 agcagaattc gcataactga aacccactct aattcaggaa agaacctccg aacaaaatat
     1561 cattagcctg cctttccagc ctttaccttc acagagtgga gccctttggc tccgtcagga
     1621 aatgatctga acaactttcc caagagagat gaggactcct ggactttgcc agtcctatcg
     1681 aacccccgaa cggttatcgt atactcacct tccttcgaag gtttccagtc atacctccag
     1741 agcgtccagg accacttgga aagcggagtc ttcagttccg tatcgtgcca cgtccttttg
     1801 tcgtcaagag aaatttgtac cttgctgata ccgtacctcc cgccaaatgc tactccgcca
     1861 ataacgtaat tgccgattgg taccgtcgtc ccatccatcg gcatcaggat ctgcgatttg
     1921 gcaggaatga ccgcatcatc agaccatccc ttcttctccc agtatccctt gaaattgtaa
     1981 ttgacaagtt ctatcttcga aagccatttg acgtgcttca ttccatagat gccgggaacg
     2041 atgaccctca gggggtaacc atgctccttt ggcaacgctt ctccattcat cctgtaggca
     2101 agaaatacgt cttccgagag tgacaaagaa tacgggatgc tgtctgaata cccatcatcc
     2161 gcataaaaag cggtcttcac gattcccttt tttgggacag ccctatctat gatacttttc
     2221 agtgtaacac catcccagag cgcattgctg attgagtcac ccccgatcgg attgccgata
     2281 cactcaaggg taacaaattc ggccttgtcc ttcatctctt cgagctcctt caagctgagc
     2341 gtgtatggtt tttcaacaag tccctctatc ctcaacctga atgcatttgg gtcaatagtt
     2401 ggcacagtat ctgaataggt ggtgatatag aattggctat tgggcgtgat ctccttggcc
     2461 ataaacccca taaaatttac cgagcctttc atgagaagcg catgtttttc ctcttccgta
     2521 gttgcctcag cgtattgata tatgccatca aatacagaaa ccccgaatcc cagtccgatg
     2581 gttcccagtc caaggccttt aaagaaatct cttctggaat agtctttggt cattcttccc
     2641 tccctaaaca ccgccatcaa tatggtttga caaaactatc taactgctcg acctccgctt
     2701 ccaatagcca atccgtaaac gaacgaaagc aactggagac cctgtattga agcgttaatc
     2761 gactctgacc ataaagctgg tggcgatcac cttgccctgg tatcccacct gcccaaatat
     2821 ctggtacaag cctgcagccg ggaaaatcat atgaacttca attttaggac cgaacttctc
     2881 ggggactgtc atcatcatct gcatgtgatg ttcatgatgc cccatagccg gcatccctgg
     2941 cagttcccca tgcgtgtgaa tgaattgcgt gagatccacc gatattatcg caagatgcat
     3001 tggtgcagag agccagggtt ctaggtcggt gacaggtttg ccattctttt tgaagatgta
     3061 gctgagtgtc acttctttcc ctgacatgat gcgctcaggc gtgctcgcaa acgtaacatc
     3121 aaggtcccca aatctcttct cccttgagaa gtcctgtttg ggaggcccca tcttaggttc
     3181 accggcaaca tcaaccagga aatgtctcga aagcggttga tcatttactg caaagtcgat
     3241 tccgatgata tagcgtccag cttttggaaa agtaaagcga acagggtata gtgcagtctt
     3301 tttcatctca ggagttatca gtccgaaatc ctgaggatgg atatgggcaa atacgctaaa
     3361 atccttgctc gagattacga catgtataag ccggtcatgg tggatagtga ggttctctac
     3421 cggctttcct tcgcgatttt tgaacgaaaa cacgacagtt gtaagcgtcc ccacaataat
     3481 acgttcaggt tctgtcttaa tgcctacaat tacctcactt cctccccctc catgcatgtg
     3541 ttgcatgcct tcgtgctgat ggccttgcat tccgctcatc tcggcaaaag cttggccttc
     3601 gcccatgaga aagagtcctc caataaacat tgtgaatgcc attgctaaaa tcttttttgt
     3661 tctattcata ttcacaacct cctttccctt caagtctcaa ccagtccatc tatctcgcgg
     3721 caactaatgg tcatttgcag ctcattttat aatccgaatc ttctaaacac ataaaacttc
     3781 gggaaatcaa taccgagcgt aaacactggc gaaaatatca aaacagtgag aacctgatac
     3841 agcgtaaggg acggaacaaa aaccccatat atacccaaca gtgtaaagcc gattatggtc
     3901 cctgcgctca gaacaaacaa ttccctcccc ggcatagatg accagaagtg cctcctttcc
     3961 ctcacaatta ataccctgaa ctggctgttg aatatcagat tcagcattac caaagtgcgc
     4021 agcttctccc atccaagatg aaaatagttc atgcccatca ggatgacgag aataccctcc
     4081 acaaccagaa gcatgccagg aacgagcgac gcaagagtaa tgttcttcac attccacatg
     4141 ttcggattag gcgtatgttt tacattgtcg gttgccagag acatggttac aaaatcgttc
     4201 gcaaagacaa gcagcgacat cccgaggaga gatagcacaa tatcatggaa ccagaagaag
     4261 ctcagggtca gcagaccgac aaattctatt acctttgtaa ctttattaat gacccacgtc
     4321 aacatccgct gataagtttg cctgcttatt attatcgcat tgattatgac acttatcccc
     4381 ggttcggtca ggactacact cgctgaagcc tttgccacgt ctgtcgcatt gcttaccgct
     4441 atccccatct ccgcctgttt cagcgccggt gcatcgttca caccgtctcc g
//
LOCUS       DATKOU010000074         6187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3172207, whole genome shotgun sequence.
ACCESSION   DATKOU010000074 DATKOU010000000
VERSION     DATKOU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3172207"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="uvrA"
                     /locus_tag="VMU21_03520"
     CDS             <1..742
                     /gene="uvrA"
                     /locus_tag="VMU21_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HUO76625.1"
                     /translation="SNPGTYLKIFDHIRKLFSEQMESRAHGYGPGFFSFNVAGGRCET
                     CSGEGYQKLEMYFFEDIYIKCEECGGKRYKPEVLRVTYNGKNIVDILDMTVDEAAEVF
                     SDIPVIFQRLSLMKDVGLGYMRLGQPATTLSGGEAQRLKICAELFSGGKGDFLYILDE
                     PTVGLHYRDVQLLIHVLRRLVDAGNTVVVIEHNLDVIAASDWVIDLGPEGGDDGGRIV
                     CEGRPEEIVNVKESYTGNYLKSVISDYK"
     gene            complement(939..1667)
                     /locus_tag="VMU21_03525"
     CDS             complement(939..1667)
                     /locus_tag="VMU21_03525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUO76626.1"
                     /translation="MPPRNYTRHLCVSDIHGCYHLLRELVEKLIMFDPATDRMTFLGD
                     YIDLSPHSMKVVLYLQTLKEKYPDNIVLLLGNHEDMAYEFLKQSQHDSFTSNAYTKLW
                     LQNGGQATLNSFGSVKVAQRILIPFIESLLPYLETNTHIFAHGGVPKDAKKLSDVPVY
                     DLIWDRNHEYGGQKTLIVGHSPYPRVKKSGNIVCIDTGAYWYGKLSAYDVLNDVVYEV
                     NKKTRDEKPSRNFTHNKTISAKMK"
     gene            1950..3608
                     /locus_tag="VMU21_03530"
     CDS             1950..3608
                     /locus_tag="VMU21_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HUO76627.1"
                     /translation="MLSYFPKRLLIALLLIISSTVITGCKGKPAATVRTVSEGNADEP
                     AYGDALVTGTIGEPSILIPMLAGDSASHDVSGLIFNGLVKYDTDLSVIGDLAESWDIS
                     SDGLVITFHLRKGIKWADGVEFSADDVMFGYRTIIDEKTPTAYSEDFRQVKKAEVPDR
                     YTFRVTYEKPFAPALTSWGNLVVLPKHLLEGKDITKSDFGRNPIGLGPYKLNRWTTGQ
                     EVMLDSYRDYFEGRPYIDRYIYKIIPDSATMFLVLQTGEIDMMGLTPVQYAKQTNTEF
                     FKKNFQKFRYPAFTYTYMAFNLKHPFFKDKRVRQAIAYGIDKDEIVDVVLFGLGSPAT
                     GPYVPNTWPYNPAVKRYEYNPEKAKQLLKEAGWKDTDGDGILDKDGRPFKFTILTNMA
                     NNPRKNTAAIIQWRLAKIGIKVEIRELEWSTFINQFIDKRSFQAAILGWSIGLDADQY
                     DIWHSSKTKEKEFNFVSYNNPEVDELLEKGRRTYDIEKRKKAYFRLQEILADELPYIF
                     LYVPDATPIVHARFKGIKASPIGISYNLPKWYVPKKLQRHTLEP"
     gene            3609..4607
                     /locus_tag="VMU21_03535"
     CDS             3609..4607
                     /locus_tag="VMU21_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO76628.1"
                     /translation="MLTYLVKRLLEMIPTLFGITLLSFVIIHLAPGKPTDIFTELNPK
                     ITPEARERLEKLYNLDKPIIVQYGLWLKKIAKLDFGESFSSDRRPVMQRIWDPKKPLL
                     ERRLFVTFMINLISMVIIFLIAIPVGISSATRRNTLYDKITTTTVFIGFAIPHFWLAL
                     LLMLLFGVTLHWLPISGLTSMNYASLPFWGKLWDLAKHLVLPIFVSAFGGLAADSRYM
                     RSSMLEVIRQDYVTVARAKGLPEREVIYRHAFRNALLPMITLVGLSVPGLIGGSVIFE
                     NIFGIPGMGQLFFQSVMTRDYPMVMGVLTIGAILTLLGNLLADIGYMLADPRIRTR"
     gene            4610..5536
                     /locus_tag="VMU21_03540"
     CDS             4610..5536
                     /locus_tag="VMU21_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO76629.1"
                     /translation="MANDTPIKLFWRRFRKNRLSVAGGIIVLALFLIATFAPLIAPCS
                     PNTIDRSHILEPPGLKHLFGTDDLGRDVMSRMIWGSRISLAVGFVAIGLATIIGMMLG
                     AISGYYGGWAERIIMRFIDIMLSIPTFFLILAVIAFIDPDIWDFILPSWLKGSSDVSF
                     LDPSILNIMIVIGLTSWMGVARLVRAEFLSLKERDFIFAARALGAGDLRIIFRHIMLN
                     SMAPVLVSAVLGIAGAVLVESSLSFLGIGVRPPTPSWGNILTLGKDNLGVAWWLSVFP
                     GLAILITVLGYNLLGEGIRDSIDPKLWQTERR"
     gene            5616..>6187
                     /locus_tag="VMU21_03545"
     CDS             5616..>6187
                     /locus_tag="VMU21_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76630.1"
                     /translation="MPDNIIKDAAALTPDPERALKNLRSFCERNPRRADELERNIRPI
                     AFLFSCSQFLANFASVHPDVLFDALQKIHDPVQKEPLAASFGEHIHALPLQSWEALLS
                     RVRMFKKKVLLLITLRDLLGKTDIVESMSELSLLADVIVEGSLPLVRSQMKEVYGDPE
                     EDAFSVIAVGKLGSEELNFSSDVDLLYVYG"
BASE COUNT         1534 a         1486 c         1603 g         1564 t
ORIGIN      
        1 ctccaacccg ggcacctacc tgaagatatt tgaccatatc aggaagctct tctccgagca
       61 gatggagtca agggcacatg ggtatggccc cggattcttc tcctttaatg tggcgggggg
      121 gagatgcgag acatgcagtg gagaagggta ccagaagttg gagatgtatt tctttgaaga
      181 catctatatc aaatgcgaag agtgtggtgg caaaaggtat aaacctgagg tcttaagggt
      241 cacctataat ggcaagaaca ttgttgatat cctcgatatg accgttgatg aggctgcaga
      301 ggtattttct gatatccccg tcatttttca gaggctctcg ctcatgaagg atgtggggct
      361 ggggtatatg aggcttggac agcctgccac caccctttcc ggaggcgagg cccagaggct
      421 taaaatatgc gctgaacttt tttcaggggg gaagggggat ttcctgtata tactggatga
      481 acccactgta ggacttcact acagggatgt acagctgctt atccatgtgc tgaggaggct
      541 cgtggatgcg ggaaacaccg ttgtggtgat tgaacacaac ctcgatgtca ttgctgcctc
      601 ggactgggta attgatctgg ggccggaggg cggcgatgac ggggggagaa tagtgtgtga
      661 aggaagaccg gaggaaatag taaacgtcaa agaatcctat accggaaact atcttaaatc
      721 ggtgataagt gattataagt gaacggcggc ctaattacgg tctgtgaatt tctgaattca
      781 cgcggcctta tatccggccg caccaataac tccaagcatt ttgcctcgga cctggcgagc
      841 ctcttaactt tcacttttat gaataatccg gactaaagga ggtgagcaca cgagggatga
      901 ggcgctatgc aggatcatcg cagcgagtgg tgatgcagct atttcatttt tgctgaaatt
      961 gttttgttat gagtgaaatt cctggatggt ttttcatcgc gagtcttttt gttcacctca
     1021 tacactacat cgttcaatac atcgtatgcg gagagctttc cgtaccaata ggcccctgtg
     1081 tctatgcaga cgatgttgcc tgattttttt acccggggat agggtgaatg tccgacgata
     1141 agagtctttt gacctccata ttcatggttt ctgtcccaaa tgagatcata taccggtacg
     1201 tcactaagtt ttttcgcatc ctttggaaca ccaccatgcg caaagatgtg ggtgttggtc
     1261 tcaagatagg gcagcaatga ctcaataaaa ggtatgagta ttctttgggc aaccttcacg
     1321 ctcccaaagc tgttgagcgt ggcctgcccc ccgttctgca accatagttt tgtatacgcg
     1381 ttgctcgtga aagaatcatg ttgggactgc ttgagaaatt cgtaggccat atcctcgtgg
     1441 ttgccaagga ggaggacaat attgtcgggg tatttttcct ttaaggtctg gagatataac
     1501 accaccttca tactgtgcgg ggaaaggtcg atatagtcgc cgagaaaggt catcctgtct
     1561 gtcgcaggat caaacattat gagcttttcc accagttctc tcaacaggtg atagcatccg
     1621 tgtatgtcac ttacgcagag atgcctcgtg taattcctcg gcggcactat aacctccttc
     1681 tacgagaatc atgcactggt cttattaata gcacactcta tgccatatgt ccaggggaat
     1741 gctcttatgg gcaaaagggt ggttcagggg ggaaggaagt cttttggaag cggttttata
     1801 ccaccattcg tggatataga acatcaggag tttcatccat aattgttcaa tagatgacga
     1861 tgattagtca agggattagg cgcgagcatt tgaaggattt ctcgctcata tcagtcccac
     1921 ttctcactgc atttctgtat aataaccata tgctttcgta cttcccgaaa cggcttctca
     1981 tcgcgcttct gctcattatc agtagtacgg tcatcacggg ttgcaaagga aaaccggcag
     2041 caacagtccg gacagtttcg gaaggcaacg ccgatgagcc ggcatacgga gacgcattgg
     2101 taacaggcac tatcggagag ccgagcattc tcatcccgat gctcgcgggc gacagcgcct
     2161 cccatgacgt ttcaggcctg atattcaatg gactggtcaa gtacgataca gacctcagcg
     2221 ttatcggcga ccttgctgaa tcctgggaca tttcgagtga cggtcttgtc ataacctttc
     2281 atctcagaaa gggcataaaa tgggcggacg gggtcgagtt tagcgctgat gacgtcatgt
     2341 tcggctacag gacgatcata gatgaaaaaa cacctacggc atacagcgag gatttcagac
     2401 aggtaaagaa agccgaggtc cctgacagat acacattcag ggtcacctat gaaaagccgt
     2461 ttgccccggc gctcacctca tggggaaacc ttgttgttct cccgaagcac ctacttgaag
     2521 gcaaagacat cacgaagagt gattttggaa gaaacccgat cggcctcgga ccatacaagc
     2581 tcaacagatg gactaccggg caggaagtga tgctcgattc ctatagggac tactttgaag
     2641 gcaggccgta cattgacagg tatatctaca aaataatacc cgactccgca acgatgtttc
     2701 ttgtgctcca gacaggggaa atcgacatga tgggacttac gccggtgcaa tacgccaaac
     2761 agaccaacac cgagttcttc aagaaaaatt tccagaagtt ccgatatccg gccttcacgt
     2821 acacatatat ggcatttaac ctgaaacatc ctttttttaa ggacaagcgg gtgcggcagg
     2881 caatagccta tgggatcgac aaagacgaga tagtggatgt agtacttttc ggactaggaa
     2941 gcccggccac aggcccttat gtcccgaaca cgtggcctta taatccggct gtcaagagat
     3001 atgaatataa cccggagaag gcaaagcagc tcctcaaaga ggccggatgg aaggacaccg
     3061 acggtgatgg gatactggac aaagacgggc gtcctttcaa gttcacgata cttaccaata
     3121 tggcaaacaa cccaaggaag aatacagcgg ccattatcca gtggagactc gcgaaaatag
     3181 gtattaaagt cgagataagg gaactcgaat ggagcacatt catcaatcag ttcatcgaca
     3241 agaggagctt tcaggccgcg atcctcggct ggagcattgg actcgacgcc gaccagtacg
     3301 acatctggca ttcctccaag acaaaggaga aggagttcaa ttttgtgagc tacaataatc
     3361 cggaggtaga cgagcttctc gaaaaaggca gaaggacgta cgatatcgaa aaaaggaaga
     3421 aggcgtattt caggttacag gagatacttg cggatgagct tccctatata ttcttatacg
     3481 tccccgatgc cacaccgatc gtacatgccc gtttcaaggg gataaaggca tccccgatag
     3541 ggataagtta taatctcccg aagtggtacg tgccgaagaa gctccagcgg catacactgg
     3601 agccatagat gctgacgtat cttgtaaaac gtctccttga aatgatcccc acattgttcg
     3661 ggattacact gctttctttc gttattatcc atctcgcgcc gggcaaaccg acggacatat
     3721 tcactgaact caatccgaag atcacccccg aggcccggga gcggcttgaa aagctttata
     3781 accttgacaa gcccatcatc gtccaatacg gtctctggct caagaagatc gcgaaactcg
     3841 atttcggcga atctttttct tctgaccgga gacccgtcat gcagaggata tgggacccaa
     3901 agaagccttt gcttgaacgg cggcttttcg tcactttcat gatcaacctc atctcgatgg
     3961 tcattatttt tctgatcgcc atacccgtcg gcatttcctc ggcaacacgc aggaatacgc
     4021 tgtatgacaa gatcaccacc acgaccgtat ttatcgggtt tgcgatccct catttctggc
     4081 ttgcactgct tctcatgctg ctcttcggcg tcacgctcca ctggctgccg atttcgggac
     4141 tgacgtccat gaattatgcg tccctgccct tctggggaaa gttatgggac ctggcaaagc
     4201 atctcgtcct tccaatcttt gtgtcggcct ttggcgggct tgcggccgat tcaaggtaca
     4261 tgcgcagcag catgctcgaa gtgatacggc aggattacgt gaccgtggcc agggcaaaag
     4321 gtctccccga aagggaggtc atttaccggc acgcctttcg gaatgcgctc cttccaatga
     4381 tcacacttgt cgggctttcg gtccctggac tgataggagg aagcgtgatc ttcgagaaca
     4441 tcttcggtat ccccggcatg gggcagttgt ttttccagag cgtcatgaca agggattacc
     4501 cgatggtaat gggcgttttg acaatcgggg caatactgac actccttggg aatctgctgg
     4561 cggatatcgg atacatgctc gcagacccga ggataaggac acggtgacta tggcaaatga
     4621 tacacccatt aaactcttct ggcgcaggtt ccggaagaac aggctttcgg tggcgggagg
     4681 gattatcgtg ctggccttgt tcctcattgc gacctttgct ccccttattg ctccttgcag
     4741 tccgaacacc attgacagaa gccacatact tgaacctccg ggtttgaagc accttttcgg
     4801 tactgatgat ctcgggaggg atgtaatgag cagaatgata tgggggtcgc ggatatccct
     4861 cgcagtcggt tttgttgcga ttggactggc aacgatcatc ggtatgatgc tcggggcgat
     4921 ttcaggatat tacggaggat gggcggaaag gataatcatg cggtttatcg acatcatgct
     4981 ctcaatcccg acgttctttc ttattctggc cgttattgct ttcatagacc ctgacatatg
     5041 ggacttcatt ctcccctcct ggctgaaagg gagcagcgat gtcagctttc tggatccgag
     5101 catacttaat atcatgatcg taatcggcct gacctcctgg atgggtgtgg cccgcctggt
     5161 gagggccgaa ttcttgtccc tgaaggagcg cgactttatt tttgctgcga gggctttggg
     5221 cgccggcgac ctccggatta tcttcaggca tatcatgctg aacagcatgg cgcctgtcct
     5281 tgtttcggcc gtgctcggta tagcgggagc ggttcttgtc gaatcctcac tgagttttct
     5341 tggaatcgga gtccgccccc cgaccccgag ctgggggaac atccttaccc tgggcaagga
     5401 caatcttggc gtggcctggt ggctctctgt ttttcccggc ctcgcaatcc tcataaccgt
     5461 gctcggctat aaccttctcg gggaagggat cagggattcc atagacccca agttatggca
     5521 aacggagagg cgttagcaag tgcattcgtt tcacgaaaca ttatgcagaa cttagtggaa
     5581 gaccgcaggg gaaataacaa tgcatcggtt cattaatgcc ggacaatatt attaaggatg
     5641 cagccgcact cactcctgat cccgagcggg ccctgaaaaa cctccgttct ttctgcgaaa
     5701 ggaatcctcg ccgtgcggat gaactggaga ggaatataag acccatcgcg ttcctcttca
     5761 gctgcagcca gttccttgcc aattttgcct ccgtgcatcc cgacgtccta tttgatgccc
     5821 tccagaaaat tcatgatcct gttcaaaagg agcctcttgc agcatccttt ggagaacata
     5881 ttcatgcatt acctttgcag tcttgggagg cccttctcag cagggtgcgc atgttcaaaa
     5941 aaaaggttct tttattgatt accctgaggg acctcctggg aaaaactgac attgtagaga
     6001 gcatgtctga actgagtctt cttgccgatg tcattgtgga aggctcactc ccccttgtcc
     6061 gttctcagat gaaggaggtc tacggtgacc cggaagaaga tgcgttttcg gtgatagcgg
     6121 tgggaaaact gggctcagag gaacttaact tcagttcgga tgtcgatctc ctctacgtct
     6181 atgggga
//
LOCUS       DATKOU010000075        11885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3940193, whole genome shotgun sequence.
ACCESSION   DATKOU010000075 DATKOU010000000
VERSION     DATKOU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3940193"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VMU21_03550"
     CDS             complement(<1..229)
                     /locus_tag="VMU21_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76631.1"
                     /translation="MTRAGAILRAFGSEIEAAHYLVVADTPVDGIVQKISKMDIPGKA
                     HFESYIRHKWRMNHKASTLWGSYQAVRTFLAF"
     gene            complement(416..1033)
                     /locus_tag="VMU21_03555"
     CDS             complement(416..1033)
                     /locus_tag="VMU21_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76632.1"
                     /translation="MGAVNTEQIIKRIHRLARSCPGLAHEFRFIAGAVFALNSAEKIG
                     HKNRATYVNANRQHHVDELNELLKALSQGTAPPPQWEAGFYYNAAVMRIHASYERILK
                     AILDPAKKVPQKKGVSRTEKWASKIEKDISAGPLARSHLESIREEINKLKHDLYGQDV
                     TSAQKRNITDDLGNAWIALTELLNIMVDSNILPALTSRYSSLPKP"
     gene            complement(1151..1636)
                     /locus_tag="VMU21_03560"
     CDS             complement(1151..1636)
                     /locus_tag="VMU21_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76633.1"
                     /translation="MGPLSKEFVEKTWQEVSGFTPARVDKEMLNMGKNQPDLLAFLMA
                     YTDDLQQEVKELAIYIAFVVYKMFLDSSGKIPRISSGEIMARYNENERFLGSLQEAHE
                     QLIEKIVDVEVFKQPYVTRYVLEALTEDAEEDDIRIAEEDIGFLLILFKTEIEVLDKK
                     A"
     gene            complement(1664..2662)
                     /locus_tag="VMU21_03565"
     CDS             complement(1664..2662)
                     /locus_tag="VMU21_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HUO76634.1"
                     /translation="MENRLMGSWRLTTRLLLRVAVMMAGLMYCAHAVAYNGRFEKWLA
                     GLRREALSQGISGAILDEALSGLTPVPRVIQLDRSQPESSMSFEEYLKLVAPQERIEE
                     GRARLAENRPLLQEIAIRYGVQPQFVVALWSIESDFGRRMGSFSVVGALVTLAYDGRR
                     SNYFRGELLNALRILNDGDIKPHQMLGSWAGAMGQCQFMPSSFRRYAVDYDNDGRRNI
                     WESRADVLASIANYLSQHGWRGGEPWGQEVRLPSRFNEKLLGRKHRKTLGEWEALGVK
                     PTEGEQFPPSITRASLIRAGEKEYLVYDNWQTLMKWNPSVYFSTAVGYLADRINSR"
     gene            complement(2647..3300)
                     /locus_tag="VMU21_03570"
     CDS             complement(2647..3300)
                     /locus_tag="VMU21_03570"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUO76635.1"
                     /translation="MDTMDFQRLRNLMVDTQIVSRGVKDARVIAAMRKVPRHLFMDES
                     SWYKAYDDMALPVAEGQTISQPYMVAVMTELLGLDGDETVLEIGTGSGYQAAVLAELS
                     REVYTIERIQSLSDTAREVIAGLGYRNIHMKVGDGTLGWPEAAPFDRILITAAAPAVP
                     KPLVDQLSDGGILVAPVGDRFSQQLVRLQKQGGELSKDYQTPCVFVPLVGEHGWKTD"
     gene            3566..4378
                     /locus_tag="VMU21_03575"
     CDS             3566..4378
                     /locus_tag="VMU21_03575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUO76636.1"
                     /translation="MRSEEDEFLKVAIEAARCGGRIVRTNLGSISEDDISIKQASDFV
                     TRVDRESEQGIMTTLRRHFPYHSFLAEESEKDTETEGYRWIIDPLDGTTNYIHGYPAF
                     SVSIALEYRRKIRLGVILDPLRDELFSAEKGRGAFLNGEPVTVSRVGNPGDALIATGF
                     PFRKKEMTDRYLELFRNIFQRVSDLRRAGSAALDLAYLACGRCDGFFEIGLSPWDMAA
                     GSILIQEAGGIVTDFGGGPEFLATGNIVAGNSTLHGVLLDEVRKVFSLSTDL"
     gene            complement(4406..5791)
                     /locus_tag="VMU21_03580"
     CDS             complement(4406..5791)
                     /locus_tag="VMU21_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUO76637.1"
                     /translation="MKRYDVVIIGSGPAGIFSAMELMENYRGLKILMVEKGKDIDKRQ
                     CPMEIRDISCNKCPECDLLSGWGGAGAFSDGKLNLSPEVGGHLSKYVNADTLQSLIDY
                     VDNAYVRLGAPKEIYGTDTETVKDIERLASKNDLILIPSRIRHIGTDLCRGLLNRMKE
                     SLNHQVDTLFGTEAEKILTEGDKVKGVRLKSGEEIESDFVVLAPGREGSRWLEGEAKA
                     LKLTLLQNPVDIGVRVELPAPVFEHLTSVTYEPKLVYYSRKFDDKVRTFCVNPYGEVV
                     KEYLKGIWTVNGHSYTQRRTNNTNFALLVSTVFTEPFNEPISYGAYIARLANFIGQGV
                     IVQRLGDLHAGRRSTHERIAKGIIQPTLKDATPGDLSFVLPYRYLSNIIEMLEALERI
                     APGVNSRHTLLYGVEVKFYSMQLKLSRSLETEIGNLFAIGDGAGVSRGLVQASVSGVI
                     AAREILERLST"
     gene            complement(5795..6571)
                     /gene="surE"
                     /locus_tag="VMU21_03585"
     CDS             complement(5795..6571)
                     /gene="surE"
                     /locus_tag="VMU21_03585"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HUO76638.1"
                     /translation="MIILVTNDDGVHSPGLIALFKAMKEVGDAYVVAPDRERSAVGHS
                     LTLHRPLKVEQMRDHVYSINGTPTDAVVVGVSKILPQRPDIVVSGINRGGNLGDDITY
                     SGTVSAAIEGTILNIPSFAISTHGEKNFHFETAASFAVTITRYITEHPMPYDTFLNVN
                     VPNLLRGLVKGIRLTRQGKRIYDNSIQEVFSPRGEQYYWIGGGVPYWEHAEDTDIHAI
                     EEGYVSVTPIHLDLTNYTALKILKKHLHVFDVVDRPTGEV"
     gene            6789..6947
                     /locus_tag="VMU21_03590"
     CDS             6789..6947
                     /locus_tag="VMU21_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HUO76639.1"
                     /translation="MEKWKCLVCGYIYDPEAGDPDGGIAPGTPFERLPDEWTCPVCGA
                     SKDMFEKA"
     gene            7191..8597
                     /locus_tag="VMU21_03595"
     CDS             7191..8597
                     /locus_tag="VMU21_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HUO76640.1"
                     /translation="MMRVPSPSYSLTVRAAIENRVGMFSRIANAISQAGGDMGAIDIV
                     RVEKGRIVRDITVNARDERHEKKIVSSIRGVEGVKVLRVMDRTFSAHEGGKIEIHTKL
                     HIKDREDLSKVYTPGVARVCRDILEHKDHAYRYTIKGNSVAVVTDGTAVLGLGDIGPE
                     AAMPVMEGKAMIFKEFADIDAFPIALNTKDPEEIISTVRNITTPFGGINLEDISAPRC
                     FEIEGRLRKELDIPVFHDDQHGTAVVVLAGLINVSRLLKKDIRKFKVVILGAGAAGIA
                     TAWMLVAYGVEDIVVCDRAGTIYVGRKHNMNPYKAAIAGKTNPRKVKGTLSDAMKGAN
                     VFFGLAGPNTVTPDDVRRMGRDPIVFALGNPDPEIAPEDALPLVRILTTGRSDYPNQI
                     NNMLGFPGIFRGLLDARAKGVNEEVKLAAAKAIAYTIKDEDLHEDYIIPSGFDRKVVT
                     NVAHAVAEAVRKTGLARI"
     gene            8727..9917
                     /locus_tag="VMU21_03600"
     CDS             8727..9917
                     /locus_tag="VMU21_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76641.1"
                     /translation="MGRLSLAVVMCFVVSLFSATAFAADTFELVIPLPLTGKQAKFGE
                     MMKHSYEMAAEEVNAKGGIKNKKIVLGFEDSGAKPETARAIVEKLIDVKKQPIIVGEY
                     TSACAKAVAAVAEERKTPYLVVASADDNITQQNYKYVFRQNAVNAHYADSVMSFMKDV
                     VKPKTIAILYESSAFGTSGADAMEKDAQRLGIKVLLKEKYEAGSVDFKPILSKVKAAQ
                     PDVIFMVSYVMDASLLMKQIKELRLDAKLFAGGAAGFAIPEFIENAKDAADYVISATL
                     WTEDLKYPGARQYADKYKAKYGAFPSYHGASAYAAIFVVKNALERTKDWTPNSIRDAL
                     KTTDMMTAFGPIKFENKEGYQNQNFMETLVLQVQKGQFETIYPEKHASNKYIYPIPTW
                     RERR"
     gene            10012..10935
                     /locus_tag="VMU21_03605"
     CDS             10012..10935
                     /locus_tag="VMU21_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUO76642.1"
                     /translation="MVQLEVFLQTLIQGILMGGLYALIGLGMTLIMGVMKIINLAHGE
                     LMMVAMYVAYWMFVLFHIDPYLSVFIATPLLFLLGIGIQKFLINPVLKVESILPENQV
                     ILTVGVGMVLANLATIFFKSDYRSTPVSYATNAWYLSDYWKTAPIELSLSVPWTVSFI
                     FAVIITIMLWFFLARTDTGKSIRATAQDLDAALLMGVNVKLMRVVTFGIGSGLVGAAG
                     CLFIPIYYLYPALGGQFTLIAFVITILGGLGSTVGAILGGLILGVFESMTSTYLGMGW
                     APVGRFVIFVACLIFLPGGVASLLRRKTLGK"
     gene            10932..11873
                     /locus_tag="VMU21_03610"
     CDS             10932..11873
                     /locus_tag="VMU21_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUO76643.1"
                     /translation="MKKYVPLIAFALAVVLPFAGFSTYIMHMLILVLMWTTIGMSWNL
                     LGGYCGQVSFGHAAYFGVGAYTAGILYSKLGVSPWWGIPFSIIIVGIFAFILGFIVLR
                     LRGPFFALATLASGEVMRITAENLVDFTGGSLGIMIQERTWVEKIWYYYIILVITAAS
                     FFLVKKLIESKLGYYFVAIREDQDAAESLGINTTLYKTIALCLSGALTGIAGAFYTNY
                     MGYIDPNVVFALHDISIITIMIVMVGGVATYWGPFVGAIIMVFLAEFIRSIPGLGTAH
                     QTLFGILLIVMIIFLPNGIVGDFPKLRRLLGMGRTSP"
BASE COUNT         2800 a         2941 c         3071 g         3073 t
ORIGIN      
        1 agaaagcgag aaaggtcctt accgcttgat agctgcccca gagagtgctt gccttgtgat
       61 tcatcctcca cttgtggcgt atgtaactct cgaagtgcgc tttgccggga atgtccatct
      121 tggagatctt ctgaacaatg ccatcaacag gagtgtctgc tactacaaga taatgtgctg
      181 cttcgatttc gctgccgaat gctctgagaa ttgcacctgc tctggtcata aatcctcctt
      241 tttcctttag aaaaagcagg ttagataatg acccttcgta atcttaagaa tcaacttttg
      301 ttacgtatag cactggcaaa ctgacacgtt tcagttaatt gtaacagtga agattttcga
      361 aagtccacgg tagcgctgcg accagccaaa gccagcaacc tagatgcgac agtgattacg
      421 gtttaggtag ggaactatag cgggaagtaa gggccgggag aatgttcgaa tccaccatga
      481 tattgagaag ctctgtaagc gcaatccacg cattgcccaa atcgtcagtt atattgcgtt
      541 tctgtgcaga cgtcacgtct tgcccataaa ggtcatgttt cagtttgttt atttcttctc
      601 gaatactttc taggtgactt ctagcaagtg gacctgcact gatgtctttc tcgatcttgg
      661 atgcccattt ctcggttctg gatacgcctt ttttctgagg gaccttcttc gctgggtcaa
      721 gtatagcctt aagaatcctc tcatacgagg cgtgaatccg cattacagcc gcattgtaat
      781 agaaacctgc ttcccattgt ggaggtggtg ctgttccttg gctgagtgcc ttaagtaatt
      841 cgttcagttc gtcgacgtga tgctgacgat tggcattcac ataagtggct cggttcttgt
      901 gaccaatttt ctctgcagag ttgagggcaa acactgcccc tgcaatgaat cgaaactcat
      961 gggcgagtcc ggggcaggat cgtgctagtc gatgtatacg cttgataatc tgttcggtgt
     1021 tgactgctcc cacgtcaact ccattcaatt gaccagtcta acatctcata acggttgcca
     1081 gtgtaactaa cgccattata tcagacttaa aaggtgtgag gtccaggaag gcatgctcat
     1141 agttttccat ttacgccttt ttatccaaaa cttctatttc ggtcttaaaa agtatcaaga
     1201 gaaaaccgat atcttcttct gcaatacgaa tatcatcttc ctcagcgtcc tccgtaagag
     1261 cttcaaggac atatctcgtg acgtatggct gcttaaaaac ttctacgtcc acaattttct
     1321 caatcagttg ttcatgggct tcttgcagac ttcctaagaa acgttcgttt tcgttatacc
     1381 tggccataat ctctccggat gagattctgg ggatcttacc acttgagtcg agaaacatct
     1441 tataaacaac aaaggcgata taaatagcaa gctcttttac ttcctgttga agatcatctg
     1501 tgtatgccat cagaaaagcc aagagatctg gctggttttt gcccatgtta agcatctctt
     1561 tatctaccct ggccggagta aagcccgaaa cttcctgcca ggttttctcc acaaattctt
     1621 ttgataaagg ccccacgtta caagttttct cccttctgag tgttcacctg ctgttgatcc
     1681 ggtctgccag gtagcccacg gcagtggaaa agtatacgga ggggttccat ttcatgaggg
     1741 tttgccagtt gtcgtacaca agatattcct tttctccggc tcggataagc gaggcacgcg
     1801 taatcgaagg ggggaactgt tcaccttctg tgggttttac gcccaaagcc tcccactccc
     1861 ccaaagtctt acggtgtttt ctccccagca gcttttcgtt gaaacggctg gggaggcgca
     1921 cctcctgccc ccacggctct ccaccacgcc agccgtgctg ggaaaggtag tttgcgatgg
     1981 aagcgagtac gtccgcgcgg ctttcccaga tattgcgtct gccatcattg tcgtagtcca
     2041 ctgcatagcg cctgaagctt gatggcatga actgacactg acccatggcg ccagcccagg
     2101 aaccaagcat ttgatggggc tttatgtctc cgtcgttgag tatcctgagg gcattgagaa
     2161 gctcaccccg gaagtagttg ctccgtcgcc cgtcgtaggc gagggtgacg agcgcgccca
     2221 ctacagagaa gctgcccatc cgtctgccaa agtcgctctc gatgctccag agggccacga
     2281 cgaactgggg ttgaactccg tagcgtatag cgatctcctg cagaagggga cggttctccg
     2341 caagtctggc ccggccttcc tcgattcttt cctgaggcgc taccagcttc agatattcct
     2401 caaagctcat gctggactca ggctggctcc gatccaactg gatgacacgc ggtacaggcg
     2461 ttaacccgct gagcgcctca tccaggattg caccggaaat accctggctg agagcctcgc
     2521 gccgaaggcc tgcgagccac ttctcaaatc ggccattgta agcaactgca tgagcacaat
     2581 acatcagtcc tgccatcatg acagccaccc gcaggagaag gcgcgtggtc aaccgccaac
     2641 tacccatcaa tctgttttcc atccatgctc ccccacaagg ggcacgaata cgcacggcgt
     2701 ctgataatcc tttgagagtt cacctccctg cttttgtaac cttacgagtt gctgggaaaa
     2761 ccgatcgccc acaggggcga ccaaaatgcc gccatcggaa agctgatcca cgaggggctt
     2821 tggtactgcg ggagcggcgg cagtaatgag aatccggtca aaaggtgcag cctcaggcca
     2881 tcccaatgtc ccgtctccca ccttcatgtg aatattgcgg tatccgagtc ctgcaatgac
     2941 ttcccttgct gtgtcagaca atgattgaat cctctctatg gtatacactt ccctggaaag
     3001 ctccgcgaga acagcggcct gatagcccga accggttcct atttcgagaa ccgtttcgtc
     3061 gccgtcaagg ccgagaagct ctgtcatcac cgccaccatg taaggctgag atatggtctg
     3121 cccctcagca acaggaagcg ccatatcatc atatgcctta taccacgagg attcatccat
     3181 aaagaggtgc ctcggtacct ttctcatggc agcgattaca cgcgcatcct tgactcccct
     3241 tgaaactatc tgtgtgtcca ccattaaatt ccttagtcgt tgaaaatcca tggtgtccat
     3301 atgccgatag tcacctccta aaaataagtg tagcagcatt ccttttcaaa ttccattgat
     3361 tttccattga aacggatagt gagcgcgttc cctgtccgcc tcaggcagac tgcggggtta
     3421 atgagtgaat atgatggaga acaatttctt acgttaaaat acctgcggct ttttcctcag
     3481 ggtttttcat taagaaagag gcgatcacgc cgtgagggtg tcgttggtgg taaaataaag
     3541 agcacgtttg ctaaaggagc gaagcatgcg atctgaggaa gacgagtttc tgaaagttgc
     3601 cattgaggcc gcacggtgcg ggggcaggat tgttcgcact aaccttggca gtatctcaga
     3661 ggacgatatc agcataaagc aggcatcgga cttcgttacg cgggtcgaca gggagtcgga
     3721 acagggaatc atgacgactc taagaaggca tttcccctat cattcctttc ttgccgagga
     3781 atccgagaag gacactgaga cagaggggta ccgctggatc attgaccccc ttgatggcac
     3841 tacgaattat atccatggat atcctgcatt ctctgtctct attgcactcg agtacagaag
     3901 gaaaatacga ttgggggtaa tccttgatcc gctccgggat gagcttttca gtgcagagaa
     3961 gggaaggggg gccttcctga atggcgagcc tgtgaccgta tcccgggtgg gtaatccggg
     4021 cgatgccctt atcgccacgg gcttcccctt ccggaaaaag gaaatgaccg acagatacct
     4081 tgaactcttc cggaatatct tccagagagt gagcgacctg agaagggccg gctctgcagc
     4141 ccttgacctt gcctatctcg cctgcgggag gtgtgacgga ttctttgaaa tcggcctcag
     4201 cccatgggac atggctgcgg ggagcattct catacaggaa gcgggaggaa ttgtgaccga
     4261 ttttggcgga gggcctgaat ttctcgccac agggaatatc gttgcaggga atagtactct
     4321 tcacggtgta ttgctggatg aggtgaggaa agtattctca ctcagcactg acttgtgagg
     4381 ggaaggacag ggaactttgc gccggctaag tgctgagccg ttccagtatc tcccttgcag
     4441 caatgacgcc tgagacagag gcctgcacga gtcctctact cacgccggct ccatccccta
     4501 tggcaaagag gttgccgatt tctgtctcga ggctcctgga gagcttcaac tgcatggagt
     4561 agaatttgac ctctacccca tagagaaggg tgtggcgtga gttcaccccc ggggcgattc
     4621 tctcaagggc ttcaagcatt tcaatgatat tcgagaggta gcggtagggc agaacaaagc
     4681 tgaggtcgcc tggggtagcg tctttcagag tcggctgtat gatccccttt gctatcctct
     4741 catgggttga gcgtcttcct gcgtggaggt ctcccagtct ctggacaatc accccttgac
     4801 cgataaagtt cgcgagtctc gcaatatagg cgccgtaaga gatgggctca ttaaagggtt
     4861 ctgtgaagac ggtgctcacc aggagcgcaa aattggtgtt gttggtcctt ctctgggtat
     4921 agctgtggcc attcactgtc catattcctt taaggtattc ctttaccact tccccgtagg
     4981 ggtttacgca gaaagtcctc accttgtcat caaattttct tgagtaatac acgagcttgg
     5041 gttcatacgt tacagaggtg agatgctcga atacaggagc aggaagttcg acccttacac
     5101 caatatccac gggattctgg agcagggtga gtttaagggc ctttgcctca ccttccagcc
     5161 atcgggagcc ttccctcccg ggtgcgagga ccacaaaatc tgattcaatc tcttctccgc
     5221 tctttagacg gacacctttc accttgtcgc cctcagtgag gattttttcc gcctcggtgc
     5281 caaagagcgt atcgacttgg tgatttaagg actccttcat cctgttgaga agtcctctgc
     5341 aaaggtcggt cccgatgtgc cttatcctcg aagggatgag gataaggtcg tttttcgatg
     5401 caagcctttc aatatctttt acggtctcgg tgtcagtgcc atagatttcc ttaggcgcac
     5461 ccaaccttac atatgcattg tccacatagt cgatgaggga ttggagggtg tcagcgttca
     5521 catattttga caggtgtcca cccacctcag gagagaggtt tagcttgccg tcgctgaagg
     5581 cgccggcccc tccccagcca ctcaggaggt cgcactcagg acatttgttg caggatatgt
     5641 ctcttatctc catgggacat tgccgcttgt ctatgtcctt gcctttttcc accataagga
     5701 tcttgagtcc tcgatagttt tccatgagtt ccatggctga gaagatccct gccggccctg
     5761 aaccaataat caccacgtcg tatcttttca tacgctaaac ttctcctgtc ggcctgtcga
     5821 ctacgtcaaa gacatgcagg tgttttttga ggattttgag ggcagtataa ttggtgagat
     5881 cgagatgtat gggggtaaca gagacatagc cctcttcaat ggcatgaata tcggtgtctt
     5941 ctgcgtgttc ccagtagggg acccccccgc cgatccaata atactgctct ccccgggggg
     6001 agaacacttc ttgtatagag ttgtcataga tacgttttcc ctgtcgtgta agtcttatcc
     6061 ctttcacgag gcccctgagg aggttaggga cattcacgtt aagaaacgta tcatagggca
     6121 taggatgttc cgtgatatag cgggtaatag tgacggcaaa ggatgccgcg gtctcaaaat
     6181 ggaagttctt ttctccgtgc gttgaaatgg caaaagaggg gatattcagg atcgttccct
     6241 ctatggcggc agaaacagtg ccggaatagg tgatatcatc gccgaggttt cctcccctgt
     6301 tgatcccgga taccacgata tcgggtctct gaggaagtat cttgctcacc cccaccacta
     6361 ccgcgtcggt gggcgtccca ttgattgaat atacatggtc cctcatctgc tccaccttga
     6421 gtggcctgtg gagggtaagg gaatgcccca ccgcgctcct ctctctatcg ggggcgacaa
     6481 cgtacgcatc tccaacctct ttcattgcct taaaaagcgc gatgagcccc ggggagtgta
     6541 cgccgtcatc attggtgaca aggattatca tctgatgctc acgttctgca ctattcttat
     6601 tttctcccct gatgtcttct ccgtatcaca ggcctttttt cacataagaa ttatatcacg
     6661 ttgagatttt tcttgacagg caatggtcat tcctaataaa gttaaaacgc ttccagatgc
     6721 tgcagggttg tgcgcacaaa cgggaggcga aatccacaat ctcaaacgaa ttaaggaggt
     6781 tccccaatat ggaaaaatgg aaatgcttgg tgtgcggcta tatttatgat ccggaagcag
     6841 gcgacccaga tggagggatt gcaccgggta caccttttga gagactcccg gatgagtgga
     6901 cctgtcctgt gtgcggtgca tcgaaggaca tgttcgagaa ggcatagaaa gagcgtaaag
     6961 ctgggggagg atgatatctt ccctccagtg catgcctgcg ctgccttaag gtctcaagaa
     7021 aagaagcatg acgctgactt ctcagccttc cctcgctccc cttcgccatc tcccaattaa
     7081 aggtctctgt tcccgctaaa gacccccttt tcttgtgtca ttgaaatagg ggcattaatt
     7141 tgatatcgtt ataactggtc ctgcttttgc gggtccgagg gaggttagaa atgatgcgag
     7201 tcccaagtcc aagctacagt ctgacggttc gtgcggccat agaaaaccgt gtcggtatgt
     7261 tctcccgcat cgcgaacgcc attagccagg cagggggaga catgggtgcc attgacattg
     7321 tccgtgtgga aaagggccga atcgtcaggg atattacggt caatgcccgt gatgagcggc
     7381 acgaaaagaa gattgtcagt tctatcaggg gtgtggaggg tgttaaggta ctcagggtta
     7441 tggacagaac cttctctgcc catgagggcg gcaagattga aattcatacc aagctgcaca
     7501 taaaggacag ggaagacctc tccaaggtat acaccccggg cgtggcaagg gtctgcaggg
     7561 atatcctgga gcataaggac catgcgtacc gatatactat caaggggaat tccgttgctg
     7621 tggttactga tggtactgcc gttctcggtc tgggcgatat tgggcctgag gcggcaatgc
     7681 cggtgatgga gggcaaggct atgatattca aggaattcgc agacatcgat gcctttccca
     7741 ttgcattgaa cacaaaagat cccgaggaaa tcatcagcac agtcaggaat atcacaacgc
     7801 ccttcggagg catcaatctg gaggatatct ctgcgccgcg ctgttttgag atcgagggac
     7861 ggttgaggaa ggagcttgat atcccagtgt ttcacgatga ccagcatgga actgcagtgg
     7921 tcgttctcgc agggcttatc aatgtttcac ggctgctgaa aaaagatatc aggaaattca
     7981 aagtggtcat cctgggggcc ggtgctgcag ggattgcaac cgcatggatg ctggtggcat
     8041 acggagtaga agacattgtc gtctgcgaca gggcgggaac gatctatgtg gggagaaaac
     8101 ataacatgaa cccttacaaa gcggcaattg caggaaagac taatcccagg aaagtaaaag
     8161 gcactctctc tgatgccatg aaaggggcaa acgttttttt tggtcttgca gggcctaata
     8221 ccgtaacccc cgatgatgta cgaagaatgg gacgggatcc catagtcttc gcgctcggca
     8281 atcctgaccc tgagatagcc cctgaggatg ctcttcccct ggtgaggata ctgactacag
     8341 gacggtctga ttatcccaac cagataaaca acatgctcgg tttcccaggt attttcaggg
     8401 ggttgctgga tgccagggcc aagggtgtca atgaagaggt gaagcttgct gctgcgaaag
     8461 ccatagcgta taccattaag gacgaagatc ttcatgagga ctacatcatc ccaagcggtt
     8521 tcgaccggaa ggttgtcacc aatgtcgccc atgcggttgc cgaggctgtg aggaagacag
     8581 ggctcgcgcg aatttgaggg tttctgtaaa taataacgtt atagtttttt ctaatagata
     8641 gtttataatg agtaacacta aacccggaaa tggttgaaca tagtaacccc ggggaatatt
     8701 actttttgtc aaaggaggat gttaccatgg gacgtctttc acttgctgtg gtgatgtgtt
     8761 ttgttgtgag tcttttctca gccactgcgt ttgcggcaga tacctttgaa ctggtcatcc
     8821 cgctgcccct tacaggaaaa caggcgaaat tcggtgaaat gatgaagcac tcttacgaaa
     8881 tggctgctga ggaggtcaat gcgaagggtg gcattaagaa taagaagatt gtcctcggat
     8941 ttgaagattc cggtgcgaaa ccagagactg caagggcgat tgtggaaaaa ctcatcgatg
     9001 taaagaaaca gcccatcatt gtgggtgagt atacatccgc gtgcgcaaag gcggttgctg
     9061 cagtggctga agagaggaag acaccgtacc ttgtggttgc gagcgccgat gacaatatca
     9121 cccagcagaa ctacaaatat gtgttccgcc agaatgccgt gaatgcccac tacgccgaca
     9181 gtgtcatgtc cttcatgaag gatgtggtga agccgaagac aatagcgatc ctctatgaga
     9241 gttctgcgtt cggcacctca ggggcggacg ccatggaaaa ggatgcacag aggcttggga
     9301 tcaaggtgct gctcaaggag aaatatgagg caggttctgt tgatttcaag ccgatactct
     9361 caaaagtcaa agcagcgcag ccagatgtga tttttatggt ctcctatgtt atggatgcgt
     9421 cactgcttat gaaacagatc aaggagttac ggctggatgc aaagctcttt gccgggggcg
     9481 cagcaggatt tgccatccct gaatttatcg aaaatgctaa ggatgcggca gactatgtga
     9541 tcagcgccac actctggacc gaagacctga agtacccagg tgccaggcaa tatgcggata
     9601 aatacaaggc gaaatacggt gcatttccct cttaccacgg ggcatcggca tatgcggcaa
     9661 tctttgtggt gaagaacgcc ctcgaaagaa ccaaggactg gacgcccaac agtattcgcg
     9721 atgcgttgaa gaccactgat atgatgacgg cctttggtcc tattaagttc gagaacaagg
     9781 agggatacca aaaccagaac ttcatggaaa ccctcgtctt acaggtgcag aagggtcagt
     9841 tcgagacgat ctaccctgaa aaacatgcaa gcaataaata tatctatcct atcccgacgt
     9901 ggcgagaaag aagatagcaa cagagcgtaa gagtaacggg gtgtcaaggg gcatagtctt
     9961 tgacaccccg ttactgtggc tttgtgattc ccctatgcta cggagagaca agtggtccaa
    10021 ttagaggttt tcctccagac cctcattcag ggaatactca tgggcggtct ctatgccctc
    10081 atcgggctcg ggatgactct tatcatgggc gtgatgaaga tcatcaacct cgcccacggt
    10141 gagctcatga tggttgcaat gtatgtcgcc tactggatgt tcgtgctctt ccatattgac
    10201 ccgtacctgt ctgtgtttat cgcaacacct cttctgttcc tccttggcat cgggatacag
    10261 aaattcctca tcaacccggt gctcaaggtg gaatccatct tgcctgagaa ccaggttatt
    10321 ctcacggtgg gagtgggcat ggtattggcg aatcttgcga ccatattctt taagtccgat
    10381 tatcgttcca cgccggtcag ctatgcaacc aatgcctggt acctcagcga ttactggaag
    10441 acagctccca ttgaactctc cctgtcagtt ccatggacag tttcttttat ctttgcggtt
    10501 atcattacca tcatgctctg gttctttcta gccaggacgg acaccggaaa gtcgatacgg
    10561 gccactgccc aggaccttga tgctgctctc cttatgggcg tgaacgtaaa gctgatgcgc
    10621 gtggtcacct ttggaatcgg ttccggactt gtgggcgcgg caggatgcct ttttatcccc
    10681 atttattacc tctaccctgc cctcggcggg cagttcacac tcatcgcctt tgtcatcacg
    10741 atccttggag ggctgggctc cactgtgggt gcaatccttg gcggtctcat actgggtgtc
    10801 tttgagtcca tgacctccac ctatctcggg atggggtggg caccggtggg gaggttcgtg
    10861 atctttgtcg cttgcctcat attcctgcca ggcggcgttg catctctcct gaggaggaag
    10921 actctgggta aatgaaaaaa tacgttcctc ttatcgcctt tgcccttgcc gtggtcctac
    10981 ctttcgcagg attcagtacc tacatcatgc atatgctgat actcgttctt atgtggacaa
    11041 ccataggtat gtcatggaac ctccttggcg ggtactgcgg ccaagtgtcc ttcggtcacg
    11101 ctgcctattt cggcgtgggc gcgtacactg caggtatcct ctactcaaag ctgggtgtat
    11161 caccctggtg gggaatcccg ttcagcatta tcatcgtcgg catctttgcc ttcatccttg
    11221 ggtttatagt cctgaggttg aggggacctt tttttgcact tgcgaccctg gccagcggtg
    11281 aggttatgag aattactgcg gagaaccttg tggacttcac cggaggctct ctcggcatca
    11341 tgatacagga gaggacctgg gtcgagaaga tatggtacta ctatattatt ctggtgataa
    11401 ccgcggccag tttcttcctt gtaaagaaat tgattgaatc aaagctggga tattacttcg
    11461 tggctatcag agaggaccag gacgctgcgg aatccctggg aataaatact acgctctata
    11521 agaccatcgc cttgtgtctg agcggggcac taacgggaat tgcgggcgca ttttatacca
    11581 attacatggg gtatattgac cccaatgtgg tctttgcact gcatgacatt tcgatcatca
    11641 ccatcatgat cgtcatggtg ggaggggttg ccacctactg gggccctttt gtgggcgcca
    11701 tcatcatggt gttcttggct gaatttatca gatccattcc cggccttggt actgcgcacc
    11761 agacgctctt cgggatcctg ctcattgtta tgattatctt tctgcctaac ggtatcgtgg
    11821 gtgatttccc aaagctcagg agacttttgg gaatgggaag gacctcacca tgagcctgct
    11881 ctccg
//
LOCUS       DATKOU010000076         6613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_481806, whole genome shotgun sequence.
ACCESSION   DATKOU010000076 DATKOU010000000
VERSION     DATKOU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481806"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1051
                     /gene="plsX"
                     /locus_tag="VMU21_03615"
     CDS             29..1051
                     /gene="plsX"
                     /locus_tag="VMU21_03615"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HUO76644.1"
                     /translation="MRVALDAMGGDFAPAVTIEGAVETVNESTDIEIVLVGDEPSIAK
                     ELSGKKYPPTRLQIRHASQIVGMDEPALAAVRKKKDSSIRRAVELVKNQEADAVVSAG
                     HSGVAMAMALFMFGASEGVHRPAIATIMPTLKGPFVLVDAGANVDCDPENLLQFALMG
                     DAYCRTMFGNPEPKIALLSIGEEDTKGNMLTKETFKLLKDSPVRFIGNIEGKDIFTGA
                     ADVVVCDGFIGNIVLKTSEGLAEVIMKMLKREIADVATGRVAYLLMKPALRNFKKKTD
                     YAEYGGAPLLGINGTCIISHGRSSAKAIKNAVKVAAEFSRKRVYEIITEEINSFRSTG
                     GKGGQG"
     gene            1038..2018
                     /locus_tag="VMU21_03620"
     CDS             1038..2018
                     /locus_tag="VMU21_03620"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HUO76645.1"
                     /translation="MVRAKIIGTGSYVPERILRNADLEGLVDTSDEWIVERTGIRERR
                     IANEKQAASDLAYEASKAALKSAGLKARDMDLIIVASVSGDMPLPATACFLQDKLDAK
                     RAAAFDVNAACSGFIYALSVADAFIRSGTYRRVLVVGAEVLSKFTDWEDRTTCVLFGD
                     GAGAVILDATEGDRGVLSVKIHSDGSMWDLLHAPGGGSRTPASKETVRKRMHFIKMKG
                     NETFKVAVRTLESLVIETLKENKLKSSDLSFLIPHQANLRIIQATAKRLNLPMEKVII
                     NLDRFGNTSAASIPIALDETVRAGRVRNGDYILLEAFGGGLTWASALIKW"
     gene            2296..3978
                     /locus_tag="VMU21_03625"
     CDS             2296..3978
                     /locus_tag="VMU21_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUO76646.1"
                     /translation="MDDLVLQIRQDGTLLEFRGQLTHTLLSSAEVLGQNVCDIMPSDI
                     AQQIMRSVERVLQTGDLHSFQYQLPTKNKNHYEVKFIVCGEDRVFALISNITQYKRAA
                     ENAKYLAYHDTLTGLPNRYLFNDRLKQAIAHAEREKQLMAILFLDIDNFKHTNDTIGH
                     KAGDVLLQSVAERLTRCLRSADSVSRLSPGESETLVARLGGDEFTMLLSDISNIQDPA
                     KVAQRILSMVAEPFIIGSHEVFITASLGIAVYPFDGKDLDTLLVNADVAMYQAKDQGR
                     NNYQYYSESMNRFTFERFSIEHKLRRALDHNEFMLFYQPQIDIRTGKMIGVEALIRWL
                     QPDLVLIKPNEFIPLAEETGLIIPIGEWVLRTACAQNKAWQKAGLNSIRMTVNVSGVQ
                     FKQQNFVEMVSQVLNDTGLAQECLQLELTESTIMKDSENTIRKLHALQAMGIQVAIDD
                     FGTGYSSLNYLKRFPLSTLKIDYSFVRDLISSSVDQSIVNAIVSLAHNFNLKVIAEGV
                     ETKKQLTFLHEARCGGVQGYLICPPVNAVSLRQFMMKESYRGILNSCGIQYG"
     gene            complement(4046..4537)
                     /locus_tag="VMU21_03630"
     CDS             complement(4046..4537)
                     /locus_tag="VMU21_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76647.1"
                     /translation="MSWQEKEREKRIEQYIESKTEGTIKKIIVKKVKEFWDSIIETNE
                     KLYPELRLENKDNVVLKGEERSLVYHENKYPQYIYLGTGHEGHISYDTEEKTLYLVRL
                     NKYYSMFRLRSKVIFFRLNHHDIDTLLRNICVDEYDYEDDLKSYRVEYTRSKSRGRRH
                     RHR"
     gene            4767..6221
                     /locus_tag="VMU21_03635"
     CDS             4767..6221
                     /locus_tag="VMU21_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUO76648.1"
                     /translation="MERLFFRKTLAELTTEVFEEKNGLKRSLGPVSLMLLGIGVIIGA
                     GIFVLTGHAAAQIAGPAIVLSFIIAAVACGFAGLCYAEFASMIPVAGSAYTYAYATLG
                     QFIAWIIGWDLILEYTLGASTVAIGWSGYVVSFLKDVGIVIPEFLSSPPVNYDHELHR
                     WISTGALFNLPAVVVVALVTVIITIGIRESAFLNAVIVFVKVAIILVFIALGVFFIRP
                     ELWHPFVPPNKGQFGTFGLSGVMRGAGVIFFAYIGFDAVSTLAQEARNPQKDMPVGIL
                     GSLIVCTVLYVTVSLVLTGVVPYTELNGPAPFAVALDAMNLHIFSPLLKVGAIAGLTS
                     VILVLLLGQTRIFFSMGRDGLLPALVSKVHPRFRTPYIVTLITGSVTACMAALLPIGI
                     VGELVSIGTLLAFVIVCTGVLVLRYTQPGARRVFKAPGGSATPVLGVLTCLYLMLGLP
                     RDTWMRLLVWLVIGLVIYFGYGMKRAGKDKSPAV"
     gene            complement(6375..>6613)
                     /locus_tag="VMU21_03640"
     CDS             complement(6375..>6613)
                     /locus_tag="VMU21_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76649.1"
                     /translation="SLALAYQYLWAPQSTAGLPSAMFSNSGHSRGHLPTALLSYQFSK
                     KLDGYLQFEYFVPENFYASKDDAIFFRWQLNYKL"
BASE COUNT         1609 a         1554 c         1776 g         1674 t
ORIGIN      
        1 gcaaggtcct tgaaatcgtc gagaaggaat gagagttgcc ctggatgcaa tggggggaga
       61 ttttgcccct gcagttacga tcgagggcgc agtagagacg gttaatgaaa gtacggatat
      121 tgagatcgtc ctcgtggggg acgaaccttc aattgcgaaa gagctttccg gcaagaaata
      181 tccgccaaca agactccaga taaggcatgc atcccaaatc gtagggatgg atgagccggc
      241 cctggccgct gtgagaaaga agaaagactc ttccatacga agggcggttg agctggtaaa
      301 aaatcaagaa gcggacgcag tggtgagcgc gggccattcc ggggttgcca tggccatggc
      361 gctttttatg ttcggggcgt cagagggggt ccacaggcct gcgatcgcca ccattatgcc
      421 cacactgaag ggcccttttg ttcttgtgga cgcgggggcg aatgtggact gcgatccaga
      481 aaacctgttg cagtttgccc tgatgggcga tgcgtattgc aggacgatgt tcggcaaccc
      541 ggaacccaag atcgcgctcc tgagcattgg agaggaagac accaagggca atatgctcac
      601 caaggagaca ttcaagctcc tcaaagattc cccggtgaga ttcatcggca atatcgaggg
      661 aaaggatatc tttacgggtg ctgcggatgt ggtggtctgc gacgggttta ttggaaacat
      721 tgtgctcaag actagtgaag gcctggctga ggtcattatg aagatgctga agcgggagat
      781 tgcggatgtg gctaccggaa gagtggcgta cctcctgatg aagcctgcac tcagaaattt
      841 caagaaaaag accgattacg cggaatacgg gggagctccg cttttgggga taaacggtac
      901 ctgcatcata agccatggga gatcctcggc aaaggcaata aagaacgccg tcaaggttgc
      961 tgctgaattc tccaggaaga gggtgtatga gatcattact gaggagataa atagttttcg
     1021 ctcaacggga gggaagggtg gtcagggcta aaatcatcgg aactggctcg tatgtgccgg
     1081 agaggatcct ccgcaatgct gatctggaag gcctggtgga tacctcggat gaatggatcg
     1141 ttgagagaac ggggatcagg gaacggagga ttgcgaatga aaagcaggct gcatctgatc
     1201 tcgcctatga agcctcaaag gcagctctca agagtgccgg actcaaggca agggatatgg
     1261 acctcatcat tgtggcgagc gtgagcgggg atatgcccct tccggcgacg gcctgtttcc
     1321 ttcaggacaa acttgatgcc aaacgggctg ctgcatttga tgtgaacgcc gcatgctcgg
     1381 gatttattta cgccctttcg gtcgcagacg cctttatcag gagcggcacg taccgaaggg
     1441 ttcttgtggt gggtgctgag gtactttcca agtttaccga ctgggaggac aggaccacat
     1501 gtgtgctttt cggtgacggc gcgggtgcgg taatcctcga tgccactgag ggagacaggg
     1561 gagtcctctc ggtgaaaata cactcggacg gcagcatgtg ggaccttctc cacgctccgg
     1621 gcgggggatc gagaactcct gcctcaaagg aaacagtcag gaaaaggatg cattttataa
     1681 aaatgaaggg gaacgagaca ttcaaggttg cagtgagaac cctggagagt cttgtgatag
     1741 agacgctgaa ggaaaacaaa ctgaaatcct ctgatctctc cttcctgata ccccaccagg
     1801 caaacctcag gatcatccag gcaactgcga agagactcaa tcttcccatg gagaaggtga
     1861 taataaacct ggaccggttc ggcaatacat cggcggcatc cattcctata gcccttgacg
     1921 agacagtgag ggccgggagg gtgagaaacg gggattatat tctcctcgag gcttttggcg
     1981 gcggcctcac ttgggcatcg gcactgatta aatggtaaat gattaaaaag aagctactct
     2041 gtccctgaac tcccggttaa gtttcttcgc ctcattttaa gagcgcccat cagaagcaca
     2101 atccgaacac gtaagagtct tgccagccat tttgaacacc ccagaaaggt gacgagtgtc
     2161 cggaatgccc cgaattgttg ttcgggattg ccgtaatcgc ggggaagggt gccttatccc
     2221 atgctatttt tctgattgta atttgtttaa caattgctta tgttattaac aagagtctaa
     2281 ttataacttg acaccatgga cgatctcgta ctacagatca ggcaggacgg tacgttacta
     2341 gagttcaggg ggcaattgac ccataccctc ttatcatctg ccgaggttct ggggcagaac
     2401 gtatgcgata tcatgccgtc ggacatcgcc caacaaatta tgcgttccgt tgagagggtg
     2461 ctccagaccg gggacctcca cagttttcag tatcagcttc ccacaaagaa caagaaccac
     2521 tatgaggtaa agtttattgt ctgtggagag gacagggtat tcgcactcat aagcaacatc
     2581 actcaataca agcgcgcagc ggagaacgcc aagtatcttg cataccacga cacgttgacc
     2641 ggtctgccaa accgttatct ctttaatgac cgtttgaaac aggcaatcgc gcacgcggaa
     2701 cgtgaaaaac agctgatggc gatactgttt cttgatatcg ataattttaa gcatacgaat
     2761 gataccatcg ggcacaaggc gggagacgtg ctcctgcaaa gtgttgccga acggttgacg
     2821 cgatgcctgc ggagcgcgga cagcgtttcc cgcctgtcac cgggagaatc ggaaactctg
     2881 gtggcccgtc ttggtggtga tgaatttacc atgctgcttt ccgatatcag caatatccag
     2941 gatcccgcaa aagtggctca gcgcatcctc agtatggtgg cagagccttt tatcattggc
     3001 tcccacgaag tattcatcac cgcgagtctg ggcattgccg tatatccatt cgatggcaaa
     3061 gaccttgaca ccctgctcgt gaatgcggac gtagcgatgt accaggccaa ggaccagggg
     3121 aggaacaact atcagtatta ttccgagtcc atgaacaggt ttacctttga aaggttttct
     3181 attgagcaca aattacggag ggcgctggat cacaatgagt ttatgctgtt ttatcagccg
     3241 caaatagata tacgcaccgg caaaatgatc ggagtggagg cgctgatacg ctggttgcag
     3301 ccggacctgg tattgattaa gccgaatgag tttatccccc ttgccgagga aaccggactc
     3361 attatcccca tcggcgaatg ggtgctccgc accgcctgcg cgcaaaataa agcctggcaa
     3421 aaggccggcc ttaattcaat acgtatgaca gtaaatgtgt ccggcgtcca gtttaagcag
     3481 caaaactttg tggaaatggt atcccaggta ttgaacgata ccggattagc tcaggagtgt
     3541 ctgcagctgg agcttaccga gagcacgata atgaaggact ctgaaaatac catcaggaaa
     3601 ttacatgcac tgcaggcaat gggcatccag gttgccattg atgatttcgg caccggatat
     3661 tcctctctga actacctgaa gcgctttccc ctctccaccc tgaaaatcga ctattccttt
     3721 gtgagagacc ttatcagcag ctctgtcgac cagtccatcg tgaatgcgat tgtgagtctg
     3781 gcacacaact ttaacctaaa ggtgattgca gaaggcgtgg agacaaaaaa acagttgact
     3841 ttcctgcatg aggcgcgctg cggaggggtg cagggttacc tcatctgtcc acccgtaaac
     3901 gccgtttccc tcagacagtt catgatgaag gagagctatc ggggcattct gaacagctgc
     3961 ggaatacaat acggatgacc aggtggtggc tcaagttcct tatggctgtg gagtgcattt
     4021 acctggtgac tttcctgtgt tgtcatcatc gatgcctatg gcgcctgcct ctggacttgg
     4081 atctggtgta ttccaccctg taggatttca agtcatcctc atagtcatat tcatccacac
     4141 agatattcct taagagggta tcgatatcat gatgattcag ccggaaaaag atgaccttag
     4201 accgtaaacg gaacatgctg taatacttat tgagtctcac aaggtagagg gtcttctctt
     4261 cagtatcata cgaaatatgc ccctcgtgtc cggtccccag atagatatac tggggatatt
     4321 tgttttcgtg gtatacaaga ctcctttcct cccccttcag caccacgtta tccttattct
     4381 ccagtctcag ttcaggatac aatttctcat ttgtctctat aatactgtcc caaaactctt
     4441 tgactttttt tactatgatc tttttgattg tcccttctgt ctttgattca atatactgct
     4501 cgattctctt ttctctttct ttttcctgcc aggacattac ggttgtatcc tttcgtgctt
     4561 cctgcaatcg gatttcggtt taaatgtaca atcttctctc atcttttttc aagggttaag
     4621 cgaggggaat tgattcttga gtgtttctgc atgaggaaga atgatgaaaa gtcctgctgc
     4681 tttcaaaaat gggtgatatg tattatcata gctgaactaa aatcgtccgg atgacgaatg
     4741 caacgtgcag atttgaatag ctgaacatgg aaaggctttt ttttagaaaa accctggccg
     4801 agttgactac cgaggttttt gaggaaaaaa acggacttaa gaggtccctc ggtcctgtga
     4861 gccttatgct cctgggaatt ggagtgatca taggcgcagg catatttgtc ctgaccggtc
     4921 atgcagccgc gcagatagca ggacccgcca tcgttctttc attcatcatt gcagcggtag
     4981 cctgtggctt cgcagggctc tgctatgcgg agtttgcctc catgatccct gttgccggaa
     5041 gtgcatacac gtatgcctat gcgacactgg gacagttcat tgcttggatt atcggctggg
     5101 accttatcct ggaatacacc cttggggcga gcaccgtagc cataggatgg tccggttatg
     5161 tggtgagttt ccttaaagat gtagggattg ttatccctga gtttctttca tccccccccg
     5221 taaattatga ccatgaactg caccgatgga tatccacggg cgctctcttt aatctgccgg
     5281 ccgtggttgt ggttgccctg gtcaccgtaa ttataaccat agggataagg gaatcggcat
     5341 tcctgaatgc agtaatagtt tttgttaaag tggccatcat cctggtattc atcgcactgg
     5401 gagttttttt tataaggcct gaactgtggc acccttttgt tccgcccaat aagggccaat
     5461 tcggaacctt cgggttgagc ggcgtgatga ggggggcagg agtaattttt ttcgcctata
     5521 taggttttga cgcggtatcg accctggccc aggaggcaag aaatccacaa aaggatatgc
     5581 ctgttggcat tcttggcagt cttatcgtct gcacagtatt gtatgtgaca gtttcgttgg
     5641 tattgactgg tgtggtacct tacactgagt tgaatggtcc ggcccccttt gcggtagccc
     5701 tggacgctat gaatcttcat atcttctctc cccttctcaa ggtgggcgcc attgccggac
     5761 tcacctctgt gattctggta ctgctccttg gacagacgag gatatttttc tccatgggaa
     5821 gggacggtct tctccccgct cttgtatcaa aggtccaccc gcgtttccgg accccctata
     5881 tcgtcaccct catcaccggc tctgtcaccg cgtgcatggc ggccctgctt cccataggaa
     5941 tcgtgggtga gcttgtgagc atcggcactc tcctcgcatt cgtaatcgta tgcacagggg
     6001 tactggtttt gagatacact cagcccgggg caaggagggt cttcaaagcg ccgggaggtt
     6061 cggcgactcc tgttctgggg gtccttacct gtctctatct aatgctgggg cttcccaggg
     6121 atacctggat gcgtcttctg gtatggcttg tgataggcct ggtgatatat ttcggatacg
     6181 ggatgaagag ggcgggaaaa gacaaatccc cggctgtgta aagattcccc cggccattca
     6241 tctctcacct gctgtgagcg cgctgaagtt ttcccccgag tgacagcaac tttagcacca
     6301 acaaatgaat gggagaacga agagaacccc caccatctcg gcgggggttc tctttacaat
     6361 tcacgagtga gaatttagag cttgtagttg agctgccatc tgaagaatat ggcgtcgtcc
     6421 tttgaagcgt agaaattttc gggtacaaaa tattcaaact gcaggtatcc atccaacttt
     6481 ttagagaatt ggtagctcaa gagtgcggtg gggagatgtc cccttgagtg gccgctgttg
     6541 gaaaacatgg cagagggaag acctgcagtg ctctggggtg cccagagata ctggtaggca
     6601 agggcaaggc tgg
//
LOCUS       DATKOU010000077        20622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3177005, whole genome shotgun sequence.
ACCESSION   DATKOU010000077 DATKOU010000000
VERSION     DATKOU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 20622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20622
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177005"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..513
                     /locus_tag="VMU21_03645"
     CDS             289..513
                     /locus_tag="VMU21_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76650.1"
                     /translation="MKNFIMRNSEIEKAIRQYMKRSPAYNHIEGRAIAKAKIEIMTDI
                     TGHSTHRSKSFYAAITIDDEDMGEEDDEWK"
     gene            1202..1660
                     /gene="tadA"
                     /locus_tag="VMU21_03650"
     CDS             1202..1660
                     /gene="tadA"
                     /locus_tag="VMU21_03650"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HUO76651.1"
                     /translation="MEKKEEDIHFMRLALEEAGFAFSEGEVPVGALVVINGAVMAKSH
                     NLRERLNDPVAHAEIMALREAAHLTNNWRLTDATLYVTKEPCIMCAGAMVNARLGRLV
                     YGCNDSKGGGVNSLYTLLTDKRLNHQVEVVSGVLEEECVLLLKRFFEERR"
     gene            2072..2161
                     /locus_tag="VMU21_03655"
     tRNA            2072..2161
                     /locus_tag="VMU21_03655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2106..2108,aa:Ser,seq:cga)
     gene            2251..2838
                     /locus_tag="VMU21_03660"
     CDS             2251..2838
                     /locus_tag="VMU21_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HUO76652.1"
                     /translation="MKSFDEVVGFHGHSCPGLALGYRVARTALREMKRKTISEDEELV
                     AIVENNSCAVDAIQVITGCTFGKGNLVFKDYGKQAYTFLMRPSGEAIRISIDFTAPQE
                     TEEEKEMWKRYSRGDSSPEVLKAVQNRKAKKTKAILDASEGELLKVSRVSVPLPPEAK
                     IYQSISCTVCGEKVAEPKARLKDGKIACIPCSEKE"
     gene            2842..3720
                     /gene="argB"
                     /locus_tag="VMU21_03665"
     CDS             2842..3720
                     /gene="argB"
                     /locus_tag="VMU21_03665"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HUO76653.1"
                     /translation="MKKLIEKANILIESLPFIRSFYGKTFVIKYGGAAQTDDELKDSF
                     ARDVALLNFIGIRPVIVHGGGPRISETMKKMGKEPTFVQGQRVTDRETMDIVEMVLGG
                     LINKEIVALINSHGGKAVGLSGKDGGLIKAKKKLLRKSVQSGEEEAIDIGLVGEVETV
                     DPSILNSLEKDGFIPVISPIGAGSGGETFNINADYVACSLASALSADKLILLTDVQGI
                     IDKKGTVITTLRKKDVKRLMDEGTVSGGMIPKVQACTQALDHGVQKTHIVDGRVAHCL
                     LLEIFTKEGIGTEIVG"
     gene            3765..4283
                     /locus_tag="VMU21_03670"
     CDS             3765..4283
                     /locus_tag="VMU21_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76654.1"
                     /translation="MRYAIHYVCFLVIILMVGCAKPSPQVVELKKYPIDSLDGIITQS
                     GAELDKDNSSDGKGSLRIQATAPTTVRLFEVNDIDIENARLTYQARVRTKDVQGQVYL
                     EMWCRFPGKGEFFSRGLQNAATGTANWMTMETPFFLKKGEKPDLVKLNLVINGSGTVW
                     IDDIRLIKGPLQ"
     gene            4367..5161
                     /gene="mtgA"
                     /locus_tag="VMU21_03675"
     CDS             4367..5161
                     /gene="mtgA"
                     /locus_tag="VMU21_03675"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HUO76655.1"
                     /translation="MPKRKLLRRLVVLCVLLVMCIPLFCFFFMPDIQKLKRENPGKTS
                     FMEYREREWKKKEGKYNIYQEWVPISRISPYLMKAVLIAEDDKFWKHEGFDYEAIQKA
                     IEKDIKAKKFKLGGSTISQQLAKNLYLSPAKNPLRKIAEAAITWRMEKALSKRRILEL
                     YLNVVEWGEGIFGAEAASRRYFGKSSSELSPEEAARLAAVLPNPRRYNPVGDQRYVVN
                     RSSLIYSIMVRRGIVIPEYGEVAGGSISSPGNDSNDVPSERLKPAD"
     gene            5342..5527
                     /locus_tag="VMU21_03680"
     CDS             5342..5527
                     /locus_tag="VMU21_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76656.1"
                     /translation="MLIIVTIDTEVFPVGTVGRIISVVAVFVMNGQEMAVLVFELPSA
                     FGTDEAVQFKRLLPIIA"
     gene            complement(5667..7973)
                     /locus_tag="VMU21_03685"
     CDS             complement(5667..7973)
                     /locus_tag="VMU21_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO76657.1"
                     /translation="MAQRQRISHKDLKTAGNPGEAELRHHTLFEQSPDGILILDTDGR
                     ILEFNEAAHRELGYSREEFAGLRISDIDPFETPEEIRARIRDVIDRGKAQFEVRHMAK
                     NGEIRDVQVITQTIVLSGRNVFHTIWRDITKRKQSETALRESEERYRALAEAAQDIIF
                     IIDRDDRIQYINRFGAMLLGQLPENITGKPRKEIFSPDLSEQQKYNLQEVFTSGHPRY
                     VESKALFSGREIWLSTSLTPIRNQEGSVYAVLGISRNITEHRRADEELKVSRRFLETI
                     IETEPECVKLIGRDGHLLMMNRSGLNMIEADTFEQVKEQPIFPLISPKYLKAFKKLTD
                     DVFQGKTGDMVFQMTTLKGRNLWLDSRAVPLRNDRNEIIAMLAITRDITERRKLEEQL
                     RQAQKMEAVGTFAGGIAHDFNNILTAIIGYASILQMKLAENNPLRMNVEHILTSAERG
                     AQLTQRLLAFSRKQIINPKLVDLREIIRKMEHLLIRIMGENIALRTIPADEALMVTVD
                     TGQMEQVLMNLATNARDSMPDGGTLTIETVSTELDEGYAMTHGYGKPGRYAVITVSDT
                     GTGMAEETRVRIFEPFFTTKEIGKGTGLGLSIAYGIMKQHDGYINVYSEPGKGTTFKL
                     YLPQTRSEIKTAHVQESIPFLSGIETILLAEDDSGVRNLTRKVLEESGYTVIEAVDGE
                     DAVSKVTEHKERIQLVILDVVMPKKNGKEVYEEIKKTAPGIRVLFTSGYTANTMQTRE
                     FLDKGLHFIPKPVSPKQLLQKVREVLDE"
     gene            8301..9764
                     /locus_tag="VMU21_03690"
     CDS             8301..9764
                     /locus_tag="VMU21_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUO76658.1"
                     /translation="MKSAKSFVLALSLTFLGLISCPGLSSIPGIQSLAYAEEPKISKE
                     AVDILSKTSQAMAEIAAAVKPAVVNISSTRTIKTQGAQSPFFSDPFFRRFFGDEFGNA
                     NRPSERKQASLGSGVIVDRDGYVLTNNHVIKDADEIRVRLSDKREFKGKVMGADPKTD
                     LAVVKIDSDHLPTIRWGDSDRLKAGEMVIAIGNPFGLSQTVTSGIVSATGRANVGIAD
                     YEDFIQTDAPINPGNSGGALVNVRGELVGINTAIFSTSGGYQGIGFAIPTSMAKVVMD
                     SLVNKGKVIRGWLGVSVQPVTPDLAQQFGLKEEKGVLVGDVTENSPAEKSGLQRGDVI
                     VEYEGKEVNDPSSLRNLVASTTPDKEIVMKLLRDGKPKTLKVIIAELPAEKQQLSGEF
                     DTPLKGVHVQDITPDVRRNLAIPKRVTGVVITAIEEGSGAGPFLEKQDVIMEVNRKKI
                     LNAKEYESAVSRIKSSQNILVLVYRKGATIYVTLPAS"
     gene            9962..12379
                     /locus_tag="VMU21_03695"
     CDS             9962..12379
                     /locus_tag="VMU21_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUO76659.1"
                     /translation="MNENRGHKRIIERLFFVPVIFVILLVALCFLIEPLLWEGVLAKV
                     LPDSSRELREEQLEPIAKVVEKAIQEGQIPGAVVLVGNHEQVLYRKAFGYRSLVPEKQ
                     SMTEDTIFDVASLTKVVATTTAVMQLVEKGKLRLEDPVAGYWPAFKANGKRYITVRQL
                     LTHTSGLRPDLSLKRGWYGNGAALKKIIAERPVASPGKRVIYSDINFEVLGELVRRVS
                     GKPLDIYCNEHIFKPLGMKDTVFTPPPALRDRIAPTEYLHEPSGKILCGEVHDPTAYR
                     MGGVAGHAGLFSTADDLSLFARMLLDGGSYRGVRILNPRTVEKMTTPQTPLHKGPLRG
                     LGWEINSPAPHKRTLFPRGSYGHTGYTGTSLWIDPDSRTYVIILTNRVHPDGKGDACP
                     LRTDISMLVEGALGKDSIEQALAAKSVRRGERLPVKQNGKVQTGIDVLKAEKFASLAG
                     LRIGLITNHSGLDSTGHRTLDLLYHAQGMKLVAVFSPEHGLSGKMDEKIASITESTTG
                     LPVYSLYGSVLRPTERMLDGLDALVFDIQDAGVRFYTYITTMGFAMEAAARKGISFYV
                     LDRPNPLTGSIVHGPVMDQDLKSFVGYFPLPTRYGMTVGELAKMFNEENRIGVNLHVI
                     RMSGYERNFWYDETGLQWVNPSPNLRSLTQAILYPGVATVEGSNVSVGRGTETPFEIF
                     GAPWINAGELAGYLNKRKILGVSFSPEHFTPASSVYKNKLCHGVRISLNDRQSFDPAA
                     LGIEITAALHRLYKKEFQIDKTFGLIGSRRVLQEIKEGQDPRAIVMHWQESLEDFQRL
                     RAKYLLY"
     gene            complement(12309..13619)
                     /locus_tag="VMU21_03700"
     CDS             complement(12309..13619)
                     /locus_tag="VMU21_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HUO76660.1"
                     /translation="MGYSVGLTTGKVVDSVTKRPIKGAFVTMNNEVVVTDEQGIFRVK
                     ASGDKVAVRAYGYLRTEQKLASPLITLPFLTSPQQIGLTPFTPKALYLSFYGVGSRIL
                     RGSALKLMEETEVNSLVIDVKGDRGNLTYRSSIPLVSEVGGQKLIIVKDIKGLIKSLK
                     EKGVYTIARIVVFKDNPLAIARPDWAVKTASGETWHDREHLAWMDPFRKEVWDYNISI
                     AVEAAQNGFDEIQFDYVRFPDTSGVVFSMPTTEESRIGAITGFLKEARTRLTPYNVFL
                     SADIFGYVCWNLNDTKIGQRLEDLIPLLDYISPMLYPSGFQYGIPGYRVPVGNPHEIV
                     YLSLKKALERTHISPVRFRPWLQAFRDYAFDRRDFKGQAISEQIRAAEKIGSHGWMLW
                     NPRNVYTQEGLKKKDSLFANQANKADISPAAFGSLQVTPANASQ"
     gene            complement(13725..14570)
                     /locus_tag="VMU21_03705"
     CDS             complement(13725..14570)
                     /locus_tag="VMU21_03705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUO76661.1"
                     /translation="MGNVLFRTGTLRSGSSVALALVTLFLWLTAAEGEIKSMKADPAG
                     AQVPILLYHRFGPVVTDGMTVTNAVFESHLTYLRDNGYTVIPLRQLVDYYLMKGPPPP
                     SRSVVIVADDGHKSVYTDMYPLVRKYHIPVTLFIYPSAISNASYAMTWDQLRELKKSG
                     LFDFQSHTYWHPNFKKDKKRFSPSEYERSVEMQLRKSKERLEKELGVKVDMLAWPFGL
                     YDEELMGKASALGYVAAFTIERHHTSASDNPMTLPRYLMINADKGRTFEAILQGRSPQ
                     RKIIY"
     gene            14808..15593
                     /locus_tag="VMU21_03710"
     CDS             14808..15593
                     /locus_tag="VMU21_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HUO76662.1"
                     /translation="MVKLSRERLSNQVYLILKEMIANYRFKPGTRVNIEQLTKELGTS
                     RTPVWEAVHRLIQEGLLTNIPNRGVFMVELTPRTSIELYTVREALEGLAARLAVQNID
                     DRLLKKMKKNLDEQSRVVQKRDLVSYSRLDFGFHAMVYEACGNTILQEMLETIKSKIR
                     PFAMHIDPVLVQLYDEHLEIFEALKKRDPQKAERAFRVHNRHIIEQIKASLESNKWGS
                     VHGRKENVTQGTRANGNTPRYKTGRRSSDALLTLWVEGNREKS"
     gene            15618..16877
                     /locus_tag="VMU21_03715"
     CDS             15618..16877
                     /locus_tag="VMU21_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76663.1"
                     /translation="MLKRTKTVAGFLGMGLMILSLVIPSGVCAEAPAKIKVGLMFGLT
                     GAASPIGPVQLEGAKLAIKEVNAAGGVKIGGKKVPVEFVVKDDETKPDIAIRRFRELT
                     SEDKVDIIVGQTFAPISAAINKEVKKTPIGYFPVNVVAITMFEKNEMAETTFAIHGAA
                     YSIGYAGASYVADKMKLKNIVFFAPAYAFGRDQWAGAKAAFEKRGIKAEYLESPVGTS
                     DYTSYLLKIAEMKPDIVMMAHWGVDAINVLKQTYETGLKKKTKIWFDWMTNVFGSGVP
                     PEALEGVYSLMSWYYDMKGFEDAAIVKAAESFTKKFMDEYKYPPDPYAAMAYIGTKEA
                     IRGIELAQSTNPSAIAKAIMSNPKFDSMKGPGTWRVDHEPLFKYGAFVVVGKGEKERK
                     DKKWDLVKIIGAYTGEDYLPSLKELGY"
     gene            16910..17689
                     /locus_tag="VMU21_03720"
     CDS             16910..17689
                     /locus_tag="VMU21_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO76664.1"
                     /translation="MAESTLIETEKVTKRFGSLTAVDGVTFSLREGEAVGIIGPNGAG
                     KTTFFNLLTGLFPPTEGKIRLSGNDITNAPPYKRVMMGIVRTFQLVSVFDSLKAIDNL
                     VLSHVKFNPDYKTGTGFFFSNFHQEEILGPCREALRRMRISHKAEIMTSELSYGDKRK
                     LEISIALSLQPKILLLDEPFAGLSDVEIVEVLDLITKVKETLTLIIIEHKISRIVDLV
                     GRLSVMHEGRLLAEGTPKEVLCDPVVREVYWGKEEGLVCSV"
     gene            17677..18366
                     /locus_tag="VMU21_03725"
     CDS             17677..18366
                     /locus_tag="VMU21_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO76665.1"
                     /translation="MLGVKNIDVAYGHAMVLHDISLNVQSGEIVFIAGRNGAGKTTLL
                     KSIAGFLKPKNGSIVFDSQNIMGLPPEKIALSGIRYVFQDKRVFTQLTVRENLELAAY
                     PVKAKLSYAIQKVVSIYPTIEKFLDAPAGGLSGGQRQILLIGRALIGSPRLLLIDEPT
                     EGLAAGTINDVLNVLNMMRGNVSMLIVEQNLSVVGMLANRVYVMQEGKITREVVDREE
                     IRSKEKLEKCL"
     gene            18407..19069
                     /locus_tag="VMU21_03730"
     CDS             18407..19069
                     /locus_tag="VMU21_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HUO76666.1"
                     /translation="MKKQKIEACKTAVVLIEPQYDFLKPGGSMYQFIAEQLKERRVIP
                     NLVNLVNKARKKVKKIVYIPFEAFEPGFPEIDRKGPGMCGLRGLEIDMPTGWKLHGKR
                     VSGAWVKGTPGPEIIPELTPKKGDLVIRGKKTLDAFWSTSLDYTLRTNLVETVAIVGF
                     HTNWCVESTARSAYDKGYRVIVIGDCTGTDTQVEQDYAEKYIFPKIGVVMNHQEFLKS
                     LV"
     gene            19145..20113
                     /locus_tag="VMU21_03735"
     CDS             19145..20113
                     /locus_tag="VMU21_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUO76667.1"
                     /translation="MEEVHTREGRKKAGMGTLLELAAIAIAFVLLSVVIGTYRAPDFI
                     IFCIYVLGFDLLYGFMGRLSFGHMLYLGTGAYVVTLFAEHISHNPFLALPAAIMAGAL
                     VGIILGPIIVRATGACFALINLAFNQIGYFLVLVAFSRYTGGEDGMSATFSKVGFFNL
                     GSKPVMFGFSLFCLVLTYYFLKRVTTSPYGLLIKSIKENETRVKFLGYNTFIYKWITF
                     ILSTSIAAFAGALSILNYGYVTPSFIDPTRNVEVIFAALIGGPGSLYGALIGGVLYMV
                     ISNYLASYIPRWEMFLGIALLIVIFRFRKGVWGYIGDKIKRGTEAA"
     gene            20124..>20622
                     /locus_tag="VMU21_03740"
     CDS             20124..>20622
                     /locus_tag="VMU21_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUO76668.1"
                     /translation="MMSTIAYGLTIGAILYIVSVGLSLTFGTMRIVNFAHGFIYTIGA
                     YILITLLPFTGGNFIVSAVVAVVAVIPMSYLIERFIVRRLYGVSIDYAIIATYAVVLI
                     GVDLIKWIWGATPIPLADPVGKDITLFSVTLPLYRLFIIAISIVLSIALWIFFKKTVV
                     GKIVVA"
BASE COUNT         5166 a         4944 c         5424 g         5088 t
ORIGIN      
        1 atggcgtaag ccgtagaaaa taaaaatcct cccctatgtt cctggaatga aatgttcggc
       61 cacttgggat gtttcgcgat aaaggtttct tcagcttttg ttatttcata gcccaggaat
      121 tccaagcggg ccttaacctg gtcagcaatg gttgcgtcca ttctgaatcc tccctattta
      181 tgtgctcctt ggcctgaaaa attactcccc ctatatcacc tcccgcttgt ttttgccacc
      241 ctcctgccag ttataatctc atatcgccac tgcaattggg ggagtaagat gaaaaacttt
      301 ataatgcgca actctgagat cgagaaggca ataagacaat acatgaagag gtctcctgct
      361 tacaaccata tagaaggaag ggccatagca aaagccaaga tagagattat gaccgatatc
      421 accggtcaca gcactcacag atcgaagagt ttttatgctg caattaccat cgatgatgaa
      481 gacatgggcg aagaagacga tgaatggaag taacacacac ccttgaggct cgctcccttg
      541 cggtgtatgg cgtcgattcg tatcgggtaa tcgttttttc gctaaggcac taataaggga
      601 aggtagccgc cctattcatc acagcactct tcaggtctgg ggccgtcaca agaaaataaa
      661 agccatttgc aatccggatg gctgtctggt gtcttacagt atatcctctg atcccaggga
      721 cacgaccaac acctgcatgt attagcctca gaaggctttg gtcccattgc gacgagatcg
      781 ctaccaattc ccttacgatg atcactgacc tggggatagg tcaacatatt atcgggactg
      841 ttatcctcac aatagagggt aaatgatacc aataatgtaa taaataggaa aaacgagacc
      901 actattctca tcgtatgtcc ttcatgctgc tattttatct cataaatacg atttagggaa
      961 tagttgtctt acagagggcg tatcaggagg attcacttct cgtattaatg cccctggatc
     1021 acgtgcgctc ctgcaaaggg agtgcaaagc gctaaaggaa gtcccggttt tcgttgttga
     1081 cacgcatttg cccgtcatgt gtaaaataat taacctctaa ggagtgtatg cgaatcgtct
     1141 tttcacggat ttggatcctt cggggtcagg gtaaacaatg atgaactgct cacggagctc
     1201 tgtggagaaa aaagaagaag acatccattt catgaggctg gcccttgaag aggccggctt
     1261 cgcgttttct gaaggggaag tccctgtggg tgcccttgtg gtgataaatg gcgctgttat
     1321 ggcaaagtcc cataacctga gggagaggct gaatgaccct gttgcccatg ccgagatcat
     1381 ggcgctgcgc gaagctgcgc atcttacgaa caactggcgc ctgaccgatg ccacgctgta
     1441 tgtgaccaag gagccgtgta tcatgtgtgc aggcgccatg gtgaatgcaa ggctgggaag
     1501 actggtctat ggctgcaatg actctaaagg gggtggggta aacagcctct atacacttct
     1561 cactgacaag agactgaacc atcaggtgga ggtggtatcc ggagttctcg aagaggagtg
     1621 tgtcttactc ctaaaaagat tctttgagga gcggagatag acgcgttgtt cgtggtcagc
     1681 tatgcagcta tatgaaaaaa tcatcttcaa cagttttagc ccacttgatt cataagttgc
     1741 ttcatagtaa gaggctcgcc cgctccgagg caagatacaa tagccctcta tgcctctctc
     1801 tccattaagt tcacctaatt gcggtttgtg ctttgctgaa ctgacgcggc ctcatatccg
     1861 gccacaccaa taactccagg tattttgcct cagaactgga aaaccttttt agtcgcactc
     1921 ttacgaataa tcccggtcag gtgtgacaca catatttgaa gccatactga gggtttatca
     1981 tgcggatttt ttttgaatct ttctattcct aaattggatt gatttgtttt aaactaaaac
     2041 tcaccgctgt gcactattcg ttgttcattg tggagaggtg gctgagtggt tgaaggcggc
     2101 ggtctcgaaa atcgttgtgg gggcaacccc accgggggtt cgaatccccc cctctccgcc
     2161 agtgcatacc cggatcctgt tcattttttc tttctggtgc tccctagctc ttttttgcaa
     2221 aacttggtta tttaaggtac aatccaacct atgaaaagtt ttgatgaagt agttggtttc
     2281 catggtcaca gctgtcccgg actcgccctt ggataccggg ttgcaaggac agcgttacgg
     2341 gaaatgaaga ggaaaaccat ctctgaagac gaggaactgg tggcaatcgt ggagaataac
     2401 tcatgtgcgg tggatgccat acaggttatt accggatgca cctttgggaa gggaaatctt
     2461 gtcttcaaag actatggcaa gcaggcatat acattcctca tgagaccctc tggcgaagcc
     2521 ataaggatct ccattgattt cacagcgcct caggagacag aagaagaaaa ggaaatgtgg
     2581 aagagatatt cccgggggga tagttctccg gaagtcctca aggccgtgca gaaccgaaag
     2641 gcgaagaaga caaaggctat tttggatgca tccgagggcg agttgttaaa ggtttcaagg
     2701 gtgagcgtcc ccctcccgcc tgaggcgaaa atatatcaga gcatctcctg cacagtgtgc
     2761 ggtgagaagg ttgctgaacc gaaggcaaga ctgaaagacg gaaagatcgc ctgtatcccg
     2821 tgttctgaaa aggagtaatc aatgaaaaag ctcatcgaga aggcgaacat ccttatcgag
     2881 tcattgccgt ttatccggag cttttatgga aagacctttg taataaagta cggaggcgct
     2941 gcgcagaccg atgatgagtt gaaggactcg ttcgcccggg atgtggccct tctgaacttc
     3001 atcggcatca ggccggtaat cgttcacggg ggcggaccaa gaatcagcga gaccatgaag
     3061 aagatgggaa aggaacccac ctttgtgcag ggccagaggg tcactgacag ggagaccatg
     3121 gatatcgtcg agatggtgct gggaggtctc attaacaaag agattgttgc cctcataaac
     3181 agccacggcg gaaaggcggt ggggctgagt ggaaaggacg gcgggctcat taaggcgaag
     3241 aagaagcttt tgaggaagtc tgtccaaagc ggggaagaag aggcaatcga catcgggctc
     3301 gtgggtgagg tggagacggt tgacccttca atcctgaact ccctcgaaaa ggacgggttc
     3361 atccccgtga tttcacccat cggtgctggc agcggcggag aaacattcaa catcaatgcg
     3421 gactatgtgg cctgctccct tgcctctgca ttaagcgctg ataaattaat actccttact
     3481 gacgtgcagg ggatcattga taaaaaaggc acagtgatta ctacgctgcg caagaaggat
     3541 gtgaagaggc tcatggatga aggaactgtt tcaggcggaa tgattcccaa ggttcaggca
     3601 tgcacccagg cccttgacca cggcgtgcag aagacccata ttgtagacgg gagggttgcg
     3661 cactgtctcc tcctggagat attcacgaaa gaagggatag gaacagagat tgtgggataa
     3721 tacccctatc tgaaatatgc tcaatgggag aggagagaac gctgatgaga tacgcaatcc
     3781 actatgtgtg ttttttagtc atcatcctga tggtcggctg tgcgaaacct tcaccacagg
     3841 tagtggaact caagaaatat cccattgata gtcttgatgg catcatcacc caatcagggg
     3901 cagaacttga taaggataac tccagcgacg gaaagggctc cttaaggatt caggccacag
     3961 cacccacaac tgtaagatta tttgaagtca acgacattga catagagaac gcacggttaa
     4021 cctaccaggc aagggttcgt acaaaggacg tgcagggtca ggtgtatctt gagatgtggt
     4081 gccgcttccc cggcaaggga gaatttttct cgcgcggtct ccagaatgcg gccacaggga
     4141 cggcgaactg gatgaccatg gagacacctt tcttcctgaa aaaaggtgag aaacccgacc
     4201 tggtgaagct caacctcgtg atcaacgggt caggaacggt ctggatcgat gacattcgcc
     4261 tcataaaagg acctctgcaa taaaagagaa agagttttta tccagggcag tgatgcccat
     4321 gctttttgta tgcatgagga taatcccgta accagcccat tctcatatgc cgaaacgtaa
     4381 gcttttaagg cgattggtag tattgtgcgt tctcctggtt atgtgtatcc ctctcttctg
     4441 tttctttttc atgcccgata tccagaaact caagagggaa aatccaggga agacctcatt
     4501 catggaatac agggagagag aatggaagaa gaaggaggga aaatataaca tctatcagga
     4561 gtgggttccc atctcaagga tatcgcccta tcttatgaag gctgtcctca ttgcagagga
     4621 tgataaattc tggaagcacg aaggctttga ttatgaggcg atacagaagg ctatagagaa
     4681 ggacataaag gccaagaagt tcaagctggg aggaagtacc ataagtcagc agcttgcaaa
     4741 gaatctctac ctctctcctg caaagaaccc tttaaggaaa attgctgaag cggccattac
     4801 gtggaggatg gagaaggcac tttccaaaag aaggatactg gagctctatc ttaatgtggt
     4861 ggagtgggga gaggggatct ttggggccga ggctgcctca cgacgctatt tcggaaaatc
     4921 ttcgtctgag ctctcgcctg aggaggcggc cagactcgcg gcagtgcttc ccaatccgag
     4981 aaggtataac cccgtcggag atcagcgtta tgtggtgaac cgctccagcc ttatctacag
     5041 cattatggtg agaaggggga ttgtcattcc tgaatatggt gaagtcgctg ggggcagcat
     5101 ttcttctcca ggcaatgaca gtaatgatgt gcccagcgaa aggcttaagc cggcggacta
     5161 aagggttctc tggtgcagga agatttgcat agagcccacg ttttttgtcc cgaggcctct
     5221 gcgtgctttt attcaatggt gtccacgaat atcgtggctg catcctggta aggcgtccgc
     5281 tcttttacgt ggtgattcat ctccctcggg tacccagata atcacccagc ataccacgcg
     5341 tgtgctcatc atcgtgacaa tagacacaga ggttttccca gttggaaccg tcgggaggat
     5401 tatttctgtg gttgccgtct ttgtgatgaa cggtcaggag atggcggtcc ttgtattcga
     5461 actcccttcc gcatttggca cagatgaggc cgtgcagttt aagagactgc tccctataat
     5521 cgcttgacgc acttccagaa agcttcaatt ctctcacaat ttcttccaca ctcttgccgc
     5581 tctctttttt ctgaggcgtt ttctttttgg aaaatctctg ccgattgtgt cccatatggc
     5641 ctttccggat atcctatgga tccttattat tcatccaaaa cctcacgcac cttttgcagg
     5701 agttgctttg gtgaaaccgg ctttggaatg aaatgcaatc ccttgtcaag gaactccctt
     5761 gtctgcatcg tattcgcagt gtagccactt gtaaagagta ctctaattcc gggtgcggtc
     5821 ttcttgatct cctcatacac ctctttgcca ttcttcttgg gcattaccac atcaaggatg
     5881 acaagctgta tcctctcctt gtgttccgtt accttactca cggcatcctc tccgtccacc
     5941 gcttctatca ccgtataccc ggactcctca agcacctttc tcgtgagatt cctcacccct
     6001 gaatcatcct ctgcgaggag tatggtttca atgccgctca agaaagggat ggactcctga
     6061 acgtgcgccg ttttgatctc tgaccttgtt tgtggcagat aaagcttaaa tgtggttccc
     6121 tttcccggtt cgctgtatac attgatatat ccgtcatgct gcttcattat accgtaggca
     6181 atggaaagcc ccagccccgt ccccttccct atctcctttg tggtaaaaaa aggctcgaat
     6241 atcctcaccc gtgtctcttc agccattcct gtgcctgtat cactcaccgt aatcaccgca
     6301 tacctgcccg gtttcccgta cccgtgcgtc attgcgtatc cctcatcaag ctcagtactc
     6361 accgtctcaa tggtgagggt ccccccatcg ggcatggagt ctctcgcatt cgtggcaagg
     6421 ttcatcagga cctgttccat ttgtcctgtg tccaccgtga ccataagcgc ttcatcagcg
     6481 ggtattgtcc ttaatgcgat attttctccc ataattctta tgaggagatg ctccatcttc
     6541 ctgataatct cgcgcagatc caccagcttg ggattgatga tctgctttct gctaaaggcg
     6601 aggaggcgct gggtgagctg agcgccccgt tccgctgagg tgaggatatg ctctacgttc
     6661 atcctcaacg ggttattctc agcaagcttc atctgcagga tgctcgcata ccctatgatc
     6721 gcggtaagga tattgttgaa gtcatgggca atgccacccg caaatgttcc caccgcctcc
     6781 atcttctgcg cctgtcttag ctgttcctca agcttcctcc gctcggtgat atcccgggtg
     6841 atggcaagca tcgcaatgat ttcgtttctg tcattgcgga ggggaactgc acgcgaatcg
     6901 agccagaggt ttctcccctt aagagtggtc atctgaaaca ccatatcccc tgtttttccc
     6961 tggaacacat cgtctgtgag cttcttaaat gctttgagat atttcggaga tataagtgga
     7021 aagatcggct gctctttgac ctgctcgaag gtatctgctt caatcatatt caaccctgaa
     7081 cggttcatca tcagcagatg gccatccctt ccaatgagct ttacacattc cggttcagtc
     7141 tctatgatgg tttccagaaa tcttctgctc accttcagct cttcatctgc cctgcggtgc
     7201 tcggtgatat tgcgggaaat acccaggaca gcgtatacac tgccttcctg atttctgata
     7261 ggagtcaatg aagtactgag ccatatttca cgacccgaaa ataacgcctt gctttccacg
     7321 taacgaggat ggcctgaggt aaacacttcc tggaggttgt atttctgttg ttccgaaaga
     7381 tcgggggaga agatttcctt ccgcggtttt cccgttatgt tctcaggcaa ttggccaagg
     7441 agcattgcgc caaaacggtt aatgtattgg atacggtcat cgcgatcgat gatgaagatg
     7501 atatcctgtg cagcttctgc cagcgccctg taacgctctt cgctttcgcg caatgccgtc
     7561 tcagactgtt tgcgtttggt gatgtcccgc cagatcgtat gaaagacatt gcgtccggag
     7621 agcactattg tttgggtaat aacctgaaca tccctgattt ctccattctt ggccatatgc
     7681 ctaacctcga actgggcctt tcctctatcg atcacatccc gaattctggc gcgtatttct
     7741 tcgggagttt cgaatgggtc gatgtcagag atacgaaggc ctgcaaattc ttctcttgag
     7801 tatcccagtt cccggtgggc tgcctcattg aactcgagta tccttccatc agtatccaga
     7861 atcagaatac cgtcggggga ttgttcaaac aaggtatgat gccggagttc ggcttcgccg
     7921 gggttgcctg cagtctttaa atctttatga gatatgcgtt gcctttgcgc catgtgtcaa
     7981 ccgctcatca tagtccctga atatgttttg tcgtaattgt catagagtta gtattttagt
     8041 atagatcgct tgaaaatgtc tgcgcataag gttgtgttcc gtagagtttg cagtatgacg
     8101 gattgtcgaa cgtgcagaca tgaaaaagta atagtgagtg aataatgtga agacctttag
     8161 tgccatattt ctcttagcag agaaactgtg ctgcgcaatc ttcaaaattt cttcatcttt
     8221 tcctgatacg ctacctgaat ataaaaatgt ttcagaatat tttgccgtga atcatatcat
     8281 agttttcaag gaggagatgt atgaaatctg caaaatcatt cgttcttgca ctgagcctca
     8341 ccttcctcgg acttatatcc tgccccggtc tgtcatcaat acctggcatt cagtccctgg
     8401 catatgcgga agaaccaaag atttcaaagg aggccgttga tatactttca aaaacaagcc
     8461 aggcaatggc agagatcgct gctgcggtga agcctgcggt agtcaatatt tcatctacga
     8521 gaacgataaa aacccagggt gctcagtccc cttttttcag cgatcctttt ttcagaagat
     8581 tcttcggcga tgagtttgga aacgcaaaca ggccgagtga gcgtaaacag gcaagccttg
     8641 ggtcaggggt gatcgtggac agggatgggt atgtcctcac caacaaccat gtcatcaagg
     8701 atgctgacga aataagagtg aggttgtctg acaaacgtga atttaaaggc aaagtgatgg
     8761 gtgctgatcc caagacggac cttgcggtgg tcaagataga ctcagaccat ttacccacaa
     8821 ttaggtgggg tgattctgac aggctgaagg cgggggagat ggtgatcgcc ataggaaatc
     8881 cctttggatt gagccaaacc gtcacttcgg ggatcgtgag tgccacagga agggcaaatg
     8941 tgggtattgc agactatgag gacttcattc agaccgatgc gcccatcaat ccggggaact
     9001 ccggcggcgc gttggtgaat gtaaggggtg agcttgtggg gatcaacacc gccatattca
     9061 gcacctcggg cggataccag gggatcggct ttgccatacc gaccagtatg gcaaaggtcg
     9121 ttatggacag cctggtaaac aaggggaagg tgatcagggg atggcttggt gtttccgtgc
     9181 agccggtgac tcctgacctg gcacaacaat tcggtctcaa ggaggaaaag ggagtccttg
     9241 tgggggacgt tacggaaaac agcccggccg aaaagtcggg actgcagagg ggtgatgtga
     9301 tcgttgaata tgagggaaaa gaggtgaatg acccctcaag cctcaggaat ttagtggcaa
     9361 gcactacgcc ggataaagag atcgtgatga agctcctgcg tgatggtaag cctaagacac
     9421 ttaaagtcat tatcgctgaa ctgcctgctg aaaaacaaca gctgtcaggg gaattcgata
     9481 ctcctctcaa aggagttcat gttcaggaca tcactcccga cgtaaggagg aatcttgcca
     9541 tccccaagag ggtcaccggg gtagtaatta ccgctattga ggagggcagc ggtgccgggc
     9601 catttctgga gaaacaggac gtgataatgg aagtgaacag aaaaaagatt ctcaatgcga
     9661 aggagtatga gtcagcagtt tcaagaatca aatcttccca gaatatactt gtgttggtct
     9721 acaggaaagg cgcaacaatt tatgtaacac tgccggcatc ataagctacg gaacaggtct
     9781 cccccagggt gcggcaggcc cctccctaga aacctgcctc acccaccctg aaacgccatc
     9841 tgccactcta gccttattaa tgaacaacca tgcaggagat cacaggcttg cgctctattt
     9901 cattctcaga gaaaaaatct gctataatgg tcttaggttt ttggttggta tgggtcgcgt
     9961 tatgaatgag aatagaggtc acaaaagaat tatagaaaga ctattcttcg tccccgtcat
    10021 ttttgtaatc ctcctcgtcg ccctatgttt tctgattgaa cccctgctgt gggagggagt
    10081 gctggccaag gtgcttccag attcgtcccg ggagctgagg gaagagcagc tggagcctat
    10141 tgctaaggtt gtggagaaag cgattcagga gggccagatc cccggcgcgg tagttctggt
    10201 gggaaatcat gaacaggttc tttaccgcaa agcgttcggt tatcgttccc tggtgccgga
    10261 gaagcagagc atgacagagg ataccatttt tgacgttgct tccctcacca aggtggttgc
    10321 caccaccacg gcggtgatgc agctggtgga aaagggcaaa ctgagactgg aagatcctgt
    10381 tgccggctac tggcctgcct ttaaagcgaa cggaaagaga tacattactg tgcggcaact
    10441 ccttacgcat acttccggcc tgcggcctga cctgagtctc aagcgcgggt ggtatggaaa
    10501 tggcgctgcc ctcaaaaaga ttattgctga gagacccgtt gcctctcccg ggaagcgtgt
    10561 catttacagt gacataaatt ttgaagtgct tggtgagctc gtccgccggg tctcgggcaa
    10621 gcctttggat atctattgca acgagcatat ctttaagcct ctggggatga aggatacggt
    10681 atttaccccc ccgccggcac tgcgcgaccg cattgcaccc acagaatatc tccatgaacc
    10741 ttcagggaag atactctgtg gagaggtcca tgatcccacc gcataccgga tgggtggtgt
    10801 ggcgggacac gcaggactgt tttcgactgc agacgacctt tcgctatttg cccggatgct
    10861 cctcgatgga ggcagttata ggggagtacg tatcctgaac ccgcgtacgg tcgagaagat
    10921 gactactccc cagacacctc ttcacaaggg cccgctgagg ggactgggct gggaaatcaa
    10981 ttctcccgca cctcacaagc gtaccctttt cccccggggc agttacggcc ataccggtta
    11041 caccggtaca tcgctctgga tcgatcccga ttcaagaact tacgtaatca ttctgacgaa
    11101 ccgggttcat cctgatggca aaggtgatgc ctgtcccctg cgaacggaca tatcgatgct
    11161 tgtggaagga gcgctgggga aggattccat tgagcaggcg cttgctgcca agagcgtgcg
    11221 aaggggcgag cgcctgcccg tgaaacagaa tgggaaagtg cagaccggaa tagacgtgtt
    11281 gaaggctgaa aagttcgctt ctcttgcagg actgcgcata gggctcatca ccaaccattc
    11341 cgggcttgat tctacagggc atcgcactct tgacctgttg tatcatgcac agggaatgaa
    11401 actggtcgcg gtcttcagcc ctgaacacgg tctctcagga aagatggatg aaaaaattgc
    11461 ttcaatcacc gagagcacaa cgggtctccc tgtatacagc ctttatggta gcgttctccg
    11521 ccctacagag aggatgctgg atggtctgga cgcattggtc ttcgatatcc aggatgcagg
    11581 agtacggttt tatacctata tcaccaccat gggctttgcc atggaggcag cagccaggaa
    11641 gggaatctct ttttatgtcc tggaccggcc gaatcccctc accggatcaa tagtccatgg
    11701 tccggtcatg gatcaggact tgaaatcctt tgtgggatat tttccccttc ccaccagata
    11761 cggcatgact gtgggggagc ttgcgaagat gttcaatgaa gagaacagga ttggtgtcaa
    11821 cctgcatgtg atcaggatga gcgggtatga acggaatttc tggtatgatg aaaccggact
    11881 ccagtgggtg aacccctcgc caaacctccg gagccttacg caggccattc tttatccggg
    11941 agtggccaca gtggagggtt caaatgtgag cgtgggaagg gggaccgaga cgccgtttga
    12001 aatcttcggc gctccctgga tcaatgcagg agaactggcc ggatatttga acaaacgtaa
    12061 aatcctcggc gtatccttca gccctgagca cttcactcct gcgagcagtg tgtataaaaa
    12121 taaactgtgc cacggtgtcc gcattagttt aaatgatcgc cagtctttcg atcccgctgc
    12181 cctgggtatc gaaatcactg cagcgctcca ccgtctttac aaaaaggaat ttcagatcga
    12241 caagaccttt ggcctcatcg ggtcacgcag ggtgctgcag gagatcaagg aaggacagga
    12301 cccccgtgct attgtgatgc attggcagga gtcacttgaa gacttccaaa ggctgcgggc
    12361 gaaatatctg ctttattagc ctggttcgca aacagagagt ctttcttctt taatccctcc
    12421 tgggtataga cgttacgggg gttccagagc atccatccat gggaaccgat tttttctgca
    12481 gcccttatct gctcgctgat tgcctggccc ttaaagtccc tcctatcaaa ggcgtaatcc
    12541 ctgaacgcct ggagccacgg cctgaaacgg accggcgaga tgtgtgtgcg ttccaaggcc
    12601 tttttaagag acaaatacac aatttcatgg ggatttccca caggaacgcg atatccgggt
    12661 atgccgtact ggaatcctga gggatagagc attggagaga tatagtccag aaggggaata
    12721 aggtcttcca gccgctgccc tattttcgtg tcattcaaat tccagcatac atagccgaaa
    12781 atatccgcgg aaaggaacac attgtaaggc gtgagtctcg ttctcgcttc tttgagaaaa
    12841 ccggtgatag cgcctatcct gctttcctct gtagtaggca tcgaaaaaac aactcctgat
    12901 gtgtcgggaa acctgacgta atcgaactgt atctcgtcaa atccgttttg cgcggcctcc
    12961 actgcaatgc tgatattgta gtcccacact tcttttctga aaggatccat ccatgcgagg
    13021 tgctccctgt catgccatgt ctctccgctt gcagttttta ctgcccaatc aggtcttgcg
    13081 atggcgaggg gattgtcttt gaagaccaca atccgcgcta tggtatagac ccctttctct
    13141 ttcagggatt ttatcaggcc cttgatgtct ttcacgatga tgagtttctg accccccact
    13201 tcagaaacca gtggtatgga gctcctgtac gtgagatttc ccctgtctcc ctttacatca
    13261 atgacgagtg aattcacctc tgtctcttcc atgagcttca gtgctgatcc ccgcagaatt
    13321 ctggaaccta cgccataaaa ggaaagatag agggccttgg gcgtgaaagg cgtcagccct
    13381 atctgctggg gagaggtgag aaagggaagg gtgatgaggg gagaagcgag tttttgctca
    13441 gtcctcagat atccgtacgc ccttaccgca accttatccc ctgaggcctt caccctgaag
    13501 atgccctgct catcggtgac taccacctca ttgttcatcg tgacaaatgc tcccttaatg
    13561 ggcctcttgg tgacggaatc caccacctta cccgtagtga gtccgacgga atagcccatg
    13621 gtatccccag acacaaacag aaagatcgtt acaaggaaag gtataagata tgtaccagtt
    13681 ctccacatat agttttctat tctatcactg aaacgcccat cttctcaata tattattttt
    13741 ctttggggtg aacgtccctg gagtattgct tcaaaagttc tccccttatc cgcattgatc
    13801 ataagatatc ggggaagggt catggggtta tccgaggcag aggtgtggtg gcgctcaatg
    13861 gtaaacgcag ctacatatcc caacgccgac gccttaccca taagttcctc atcgtaaagt
    13921 ccgaaaggcc atgcgagcat gtccaccttg accccaagtt ctttctccaa cctctccttt
    13981 gatttcctta actgcatctc aacggacctt tcatattcag aaggcgagaa tctctttttg
    14041 tctttcttga agtttgggtg ccagtacgta tgggattgga aatcgaagag gccagatttt
    14101 tttaactccc tcagctggtc ccaggtcatg gcgtatgaag cattggatat ggcggacgga
    14161 tagatgaaca gggtaaccgg aatatggtat ttccttacca ggggatacat atctgtgtag
    14221 acagacttgt ggccatcgtc agccacaatc accaccgacc gagaaggagg aggaggcccc
    14281 ttcatgaggt aataatctac gagttgcctg aggggaatga ctgtgtagcc gttgtccctg
    14341 agataggtga gatgggactc aaagactgcg ttcgtgacgg tcataccgtc cgtaaccaca
    14401 ggcccgaagc ggtggtagag gagtatgggc acctgcgccc cggcagggtc tgctttcatg
    14461 ctctttatct ctccctctgc tgcagtaagc cataaaaaaa gagtaaccaa tgccaaggca
    14521 accgaagaac cgcttctcag tgtgcctgtt cgaaacaaaa cgtttcccat cctgcctcct
    14581 cggtgattgc tttatggccc atggcattct cctgcaatgg cgtctagaat tatacgtcat
    14641 ccgggagtaa gaagacaaag ggttcccctt gggcccacaa gagcactgcg gattacgaag
    14701 ggtatgccgg gatccgtgcg aactaatttc atgaaatatt aaaaataaaa aatgaaatcg
    14761 ccttgacaag gatccccggg ggaggtaaaa ttgtgtgggg gaatgcaatg gtgaagttga
    14821 gcagggagcg gctgagtaat caggtgtatt taatcctcaa ggagatgatt gcaaattacc
    14881 ggtttaagcc gggcacccgc gtcaacatcg aacagctgac aaaggagctt ggaacgagcc
    14941 gaacccctgt atgggaggca gtgcatcgtc tcattcagga aggtcttttg accaatattc
    15001 ccaaccgcgg cgtattcatg gtcgaactca ccccgaggac ctccattgag ctctataccg
    15061 tgagagaggc gctggaggga ctggcagcac ggcttgcggt acagaatatt gacgacaggc
    15121 tcctaaagaa aatgaagaaa aatctggatg agcaatccag ggttgttcaa aaaagagact
    15181 tggtgagcta ttcccgtctg gattttggct ttcacgccat ggtatacgag gcatgcggga
    15241 acacaattct gcaggaaatg ttggagacca taaagagcaa gatacgtccc tttgccatgc
    15301 acattgatcc tgtactcgtc caattatatg atgagcacct tgagatcttt gaagccctca
    15361 aaaagcggga cccgcagaaa gcggaacgcg ccttccgggt gcataaccgc catatcatag
    15421 agcaaataaa ggcaagtttg gagagcaata aatggggcag tgtccacggg agaaaggaga
    15481 acgtgacgca aggtacaaga gccaacggta atacccctag atataaaaca ggaaggcgct
    15541 ccagtgatgc cctgctgacc ctatgggtag agggtaatcg ggagaaatct taaccatttt
    15601 aggaaaggag gaaggtcatg ttgaaaagga cgaagacagt agcgggtttt ctcggcatgg
    15661 ggttaatgat tctgagtctc gtgattccgt cgggagtgtg tgccgaagcg ccggccaaga
    15721 tcaaggtagg gctgatgttt ggtctcaccg gcgcagcgtc accgataggg ccggttcagc
    15781 ttgagggtgc aaagctggcc atcaaggagg tgaatgcagc agggggagtg aagataggag
    15841 ggaaaaaggt gcctgtggag ttcgtggtga aggatgacga aacaaagccg gacatcgcca
    15901 tccgcaggtt cagggagctg acgagcgaag acaaggtgga cattattgtg ggccagacct
    15961 ttgcgcccat atccgctgcc atcaacaaag aggtgaaaaa gacccctatc ggatattttc
    16021 ctgtgaatgt ggtggcgatc accatgtttg aaaagaacga aatggctgag acaacctttg
    16081 cgatccatgg tgcggcatat tccatcgggt atgcaggggc ctcgtatgtt gccgacaaga
    16141 tgaaactcaa gaacattgtc ttttttgccc ctgcctacgc ctttggccgc gatcagtggg
    16201 caggtgctaa ggcagcgttc gagaagcggg ggattaaggc ggaatatctg gagtctcctg
    16261 tggggacgag cgattacacc tcctatctcc tgaagatcgc ggagatgaag ccggatatcg
    16321 tcatgatggc ccattggggt gtggatgcca tcaatgtatt gaaacagacg tacgaaaccg
    16381 gactcaagaa gaagacgaag atatggtttg actggatgac caatgtgttc ggaagcgggg
    16441 ttccgcccga ggcactggag ggggtgtatt ccctgatgtc ctggtactat gacatgaagg
    16501 gctttgagga tgcggccatt gtgaaggcag cagagagctt cacgaagaaa ttcatggatg
    16561 agtacaagta tcctccggac ccctatgctg caatggcgta tatcggaaca aaggaggcca
    16621 taaggggaat cgagcttgcc caatccacca atccctctgc cattgcgaag gccatcatga
    16681 gcaatccaaa gtttgattcc atgaagggcc ctggaacatg gagggttgac catgaacccc
    16741 ttttcaagta tggtgcattt gtggtggtgg gaaaagggga gaaggagaga aaggacaaga
    16801 agtgggacct ggtcaagata atcggtgcat acacagggga agactatctc ccttccctca
    16861 aggaactggg atattaactg aggacggagc ataaagggaa ccgtcagtca tggcagaaag
    16921 taccctcata gaaacggaaa aggtaactaa gcgttttggc agtctcactg cagtggacgg
    16981 ggtgaccttt tccctccgtg agggagaggc agtgggtatc atcgggccca acggagcggg
    17041 aaagacgacc ttctttaacc tcctcacagg cctctttccc cccactgagg gaaagatacg
    17101 tctctcgggg aacgatatca ccaatgcccc tccctataaa agggtcatga tggggattgt
    17161 gaggaccttt cagctcgttt cggtcttcga ttccctcaag gccattgata atctcgtact
    17221 ttcccatgtg aagttcaacc ctgactacaa gacaggaaca gggtttttct tcagtaattt
    17281 tcaccaggag gagattctgg ggccatgccg ggaagcctta agacggatga ggatatctca
    17341 caaggcggaa atcatgacct cggaactctc ctatggtgat aaacgcaaac tcgaaatatc
    17401 catcgccctc tccctccaac caaagatact cctcctggac gagccctttg cgggattgag
    17461 cgatgtggag attgtggagg tcctggatct catcacgaaa gttaaggaaa cactcaccct
    17521 catcatcatt gagcacaaga tatccaggat cgttgatctg gtaggccggc tgagcgtgat
    17581 gcatgaagga aggctgcttg ccgaaggaac accgaaggag gtgctctgcg accctgtggt
    17641 gagggaggta tactggggaa aggaagaggg cctcgtatgc tcggtgtgaa gaacatcgat
    17701 gttgcgtacg ggcatgcaat ggtcctgcat gacatctctc tgaacgtgca gtcaggggag
    17761 atcgtattca tcgcaggacg gaacggcgca gggaagacca cccttctgaa atccatcgca
    17821 ggctttctga aaccaaagaa cggctccatt gtgtttgaca gtcagaacat catgggtctg
    17881 ccccctgaaa agatcgcgct ctcgggaata cggtatgtat ttcaggataa gcgggtattc
    17941 acccagctca cggtgaggga gaaccttgaa cttgccgcat atccggtaaa ggcaaagctc
    18001 tcttacgcta tccagaaagt ggtgagcatc tatcccacca tcgagaagtt tctggatgcc
    18061 cctgcagggg gattgagcgg cggccagcga cagattctcc tcatcggcag ggcgcttatt
    18121 ggaagtcccc gtcttttgct cattgacgaa ccaacggaag gactggcagc cggcaccatc
    18181 aatgatgtcc tgaatgtcct gaacatgatg aggggaaatg tctccatgct catcgttgag
    18241 cagaatctgt ctgtggtggg gatgctcgcc aatagggtat acgtgatgca ggagggaaag
    18301 atcactagag aggtagttga ccgggaggag atcaggagca aggagaagct ggaaaaatgc
    18361 ctataggaca atctgtccta atagttcatc acgtaaagga ggaacaatga agaagcagaa
    18421 aattgaagca tgcaaaactg ccgttgtatt gattgaaccc cagtatgatt ttctcaagcc
    18481 gggcgggtcc atgtaccagt tcattgcaga gcagctaaag gaaaggaggg tgattcccaa
    18541 cctggtgaac ctggtgaaca aggcgaggaa gaaggtgaag aagatcgtct atattccctt
    18601 tgaggcattt gagccggggt tcccggagat cgaccggaag ggacctggga tgtgcggcct
    18661 ccgcggtctg gagatcgaca tgcccacagg gtggaagctc catggaaagc gggtgagcgg
    18721 tgcctgggta aagggtaccc cgggacctga gatcatccct gaactcaccc ccaaaaaagg
    18781 agacctggta atcaggggca agaagaccct ggatgcgttc tggtccactt ctctggatta
    18841 taccctcaga acgaaccttg tcgagacggt tgccattgtc gggttccata ccaactggtg
    18901 tgtggagtcc actgccaggt ccgcatacga caaggggtac agggtgatcg tgatcgggga
    18961 ctgtactggc acagacaccc aggtggagca ggactatgcc gagaagtaca tattccctaa
    19021 gatcggagtc gtcatgaacc atcaggagtt cctgaagagc ctggtataag aaacacggaa
    19081 gaacctgccc tgctccgaga gcagggcagg taggacacaa ctacaggaca gcaattaggc
    19141 acgcatggaa gaagtacaca ctagagaggg caggaagaag gcaggcatgg gaacgctcct
    19201 tgagctggct gccatcgcaa tcgcctttgt tctgctgtcc gtagtcatcg ggacttaccg
    19261 tgccccggat ttcatcattt tctgcattta tgtcctgggc tttgatctcc tctacgggtt
    19321 tatggggagg ctctccttcg gccatatgct ctatctcggc acaggggcat acgtggtcac
    19381 cctttttgca gagcatattt cccataaccc ctttctcgca cttcctgcag cgataatggc
    19441 aggggcactc gtagggatca tcctcggtcc cattattgtg agggccacag gcgcgtgctt
    19501 tgcccttatc aaccttgctt ttaaccagat cggttacttt cttgtcctgg ttgcgttttc
    19561 aagatatacg ggcggcgagg acggaatgtc cgcaacgttt tcaaaggtgg gttttttcaa
    19621 tttgggcagc aagccggtca tgttcgggtt ttctcttttc tgtctcgtcc tcacctatta
    19681 tttcctgaaa agggtcacca cttcgcccta cggactcctg atcaaatcca ttaaggagaa
    19741 cgagacaagg gtgaaattcc ttggatacaa tacctttatc tataaatgga tcacctttat
    19801 cctctcaacc tccatcgcgg cctttgccgg cgcattatcc atcctcaact acgggtatgt
    19861 aacgcccagc tttatcgatc ccacgaggaa tgtggaggta attttcgctg ccctcatcgg
    19921 cgggccggga agcctttacg gtgccctcat cggaggcgtc ctctacatgg tgatcagcaa
    19981 ctacctcgcc tcctatatcc cgaggtggga gatgttcctg ggaattgcgc tcctgatcgt
    20041 gattttcagg ttcagaaaag gtgtctgggg atatatcggc gacaaaatta agcgcgggac
    20101 agaggctgca taggagagat gccatgatga gcactattgc atacggactt accataggag
    20161 cgattctcta cattgtgagc gtggggctct cgctgacttt cgggaccatg aggatcgtga
    20221 attttgccca tgggtttatc tataccatcg gcgcctacat cctcatcact ctcctgccat
    20281 tcaccggggg caacttcatt gtctcagccg tggtggcggt tgtggcggtg attcccatga
    20341 gctatctcat cgagcgcttt attgtgagga ggctctacgg tgtctccatc gattatgcaa
    20401 ttattgccac ctatgccgta gtactgatcg gagtcgacct cattaaatgg atttggggag
    20461 ccactcccat ccccctcgca gaccctgtgg gaaaggacat cacccttttc tcagtgacgc
    20521 tgcccctcta ccgtctcttc atcatcgcca tctccatcgt cctgtccatc gccctctgga
    20581 tattcttcaa gaagacggtg gtgggcaaga tcgtggtcgc tg
//
LOCUS       DATKOU010000078         5740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4332642, whole genome shotgun sequence.
ACCESSION   DATKOU010000078 DATKOU010000000
VERSION     DATKOU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4332642"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VMU21_03745"
     CDS             complement(<1..513)
                     /locus_tag="VMU21_03745"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HUO76669.1"
                     /translation="MKSDSASRVGMASPETRLLLLLSRVCLSCSELDAAQVFISESLD
                     WDIFSSHSLRHGTYSIIYKNLLKLDGVPGHVLDTFRNAYNSTLWLNIQLVSELDRILF
                     DLEKRGICAIPLKGPIASEAIFGDMGLYPGGDIDILIKLEDLDGTRQYLESEGYTLND
                     KGFDEYREFFI"
     gene            complement(510..1748)
                     /locus_tag="VMU21_03750"
     CDS             complement(510..1748)
                     /locus_tag="VMU21_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76670.1"
                     /translation="MIYSSKYSDLGNSGVVYNVHIVNRYYPPNPAVTGEEASKMAAYL
                     LNCLTEVAVTVNYIDAPYMGGGSNKKPVGNLSALQPIYKGRSKLLRFLGNFLEGRKLI
                     KTALTKADIIISLTDPPLLNYWSGNLCKKKGVPWIYWSLDLYPDAFASAGLVSKNNPF
                     YRYFAASFRKNTPDLFVALGKEQARFIQKTFYRDIPSIILPCGIHNEPANETRPEWRR
                     DEGKIYFAYAGNLGEAHSHDFLIDFVRCLDPNKHRCILALYGSGASSVLREVKDNPTV
                     EIVTAISRKDLYFVDVHLVSLKPDWTHICVPSKAVSAVCAGGTILFNGSEGADTWQMF
                     HDAGWLVQECENRETRMGEIRALLKEISVLHKLSKKKEHAIRLKRELFDLESYAHKSI
                     AEWIIKTAVEGNTHYREDLP"
     gene            complement(1696..2757)
                     /locus_tag="VMU21_03755"
     CDS             complement(1696..2757)
                     /locus_tag="VMU21_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUO76671.1"
                     /translation="MSKKIMLVTGSSGLIGSEVVSVLYRGFDSVYGVDNNMRASFFGP
                     SGDTRWNQRRLESTVPNFRHVECDIRDRQGITKVIEDLRPSLIVHAAAQPSHDLAASR
                     PLEDFDTNAVGTMNLLEAARQYSPNVVFVHMSTNKVYGDAPNLIKLRELPSRWDYDDP
                     RYANGIDETFTIDQSKHSLFGASKVASDIIVQEYGRYFGMKTCCLRGGCLTGPSHSGV
                     ELHGFLSYLIKCNVEGRVYNVFGYKGKQVRDNIHSFDVAQFIYAFYQEPRIAGVYNIG
                     GGRENSISILEAFEKIETISGEKMHYEYRERNREGDHICYISNLSKIKEHYPHWSITK
                     SLDDIFLEIFRSWKQRGSV"
     gene            complement(2750..4510)
                     /locus_tag="VMU21_03760"
     CDS             complement(2750..4510)
                     /locus_tag="VMU21_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HUO76672.1"
                     /translation="MIILGLNAYHGDSSACIVVDGKLIAAVEEERFTRIKHWAGLPVQ
                     AIQYCLKETGSDIGEVDHIAINRDPKANLAKKVLFAFFKRPSSRILSDRLKNASRITD
                     LRTVLSSTFKVPRSKLKAELHTIEHHAAHLASSFFVSPFEEAAVVSVDGFGDFVGAMW
                     GIAGGSKITITHRTFFPHSLGLFYLALTQYLGFPKYGDEYKVMGLAAYGEPAFMEEMK
                     TILRMLRDGDFELDLDFFIHHSEGVSMTWESGEPHMGSVFSRKLEERLGPARKKEAEI
                     GERHKNIAASMQRRYEEVFFHILNHVYEKTRCPRLALSGGCAMNSLANGKIFQNTPFR
                     EVYIQPAAGDAGGSIGAAFYVWNQLLDKPRSFVLERPYLGPEFTTAHISSLLTAKSNE
                     LNSENCAFETIEDIKDLCLQTARAISQGKVVGWFQGKMEWGPRALGNRSIVCDPRRPD
                     MKDILNLKIKRRESFRPFAPSIHLEAVGEYFETDYPDPFMLKVYKIRPEKRDIIPAVT
                     HVDGTGRLQTVRYEDNPAYWQLIEEFRKITGVPVVLNTSFNENEPIVCTPEEALDCFL
                     RTKMDMLVLDKFIIRRMQNV"
     gene            complement(4590..5516)
                     /locus_tag="VMU21_03765"
     CDS             complement(4590..5516)
                     /locus_tag="VMU21_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76673.1"
                     /translation="MFPRDTRKITYRIRNVLKESVSGWLEFEVPKGLVIDGSKPEFGP
                     LKPGDTAEVIVTFRSNNPNEGRHVVPYRIHYRQAGEGKSIRTAAKSIVVAINALLESV
                     YQSPQPAVYQIHAPRYTVKLDMFNGLSRYLADDDDTIRLDGNPLFTFSDGNQELLSDK
                     TKTAFTWPVETPALLTASVEERARYQVLFFGDRLMVKMDPNWTQFEKTFFTLPGIWVS
                     PEGPPQWSGIMRGDRQGKDSDAQQNGTIMSVSAAELAFPRARWDLCFQFNPPQDVAFA
                     GAGMKFQLNSLKGDSWTIGFCKPGGLETWRWK"
BASE COUNT         1461 a         1430 c         1303 g         1546 t
ORIGIN      
        1 tatgaagaac tctcgatact catcaaagcc tttatcgttt aacgtataac cctctgactc
       61 aagatattgc cttgtcccgt ccaggtcttc caatttgatc agtatgtcta tatctcctcc
      121 cgggtatagg cccatgtcac cgaaaatcgc ttcagaagcg atcggtcctt ttagagggat
      181 tgcgcagatg ccacgtttct caaggtcgaa aaggatcctg tcaagttccg agaccaattg
      241 aatgttcaac cagagtgtgc tgttataggc atttctgaat gtatccagaa catgtcccgg
      301 gacaccatca agcttcagaa ggtttttata gatgatgctg taagtaccat gacgtaagga
      361 atgtgacgag aagatatccc agtccaaact ctcactgatg aatacttgtg ctgcatcgag
      421 ttcggaacat gacaggcaga cccttgagag gagaagaagt aatcttgttt ccggggaagc
      481 cattccgacc ctactggcgg aatctgactt catgggaggt cctcacggta atgggtattt
      541 ccttccacgg ccgtttttat gatccattca gcaattgact tatgtgcgta agattccagg
      601 tcaaagagtt ctcttttcaa acgtatcgca tgctcttttt tcttacttaa cttgtgaaga
      661 acggaaatct cttttaatag tgccctgatt tcccccatcc tggtttcgcg gttttcacat
      721 tcctgcacaa gccagcccgc gtcatgaaac atctgccaag tatcagcgcc ttctgatcca
      781 ttgaacaaaa ttgttccgcc ggcgcacaca gcgctgaccg cctttgaagg cacgcaaata
      841 tgggtccagt ccggttttaa gctgacgagg tgaacgtcaa caaaatacag gtcctttctg
      901 cttatggctg tcacaatttc aaccgtcgga ttatccttta cctcgcgcaa aaccgaagat
      961 gcccctgaac cgtagagtgc gaggatgcat ctgtgtttgt ttggatcaag acagcggaca
     1021 aaatcaatga gaaaatcatg gctgtgtgcc tcccccaggt tccccgcata tgcaaaatag
     1081 attttccctt catcccttcg ccactcaggt ctcgtttcgt ttgcaggctc attgtgtatc
     1141 ccgcagggaa ggataataga gggaatatct cgataaaatg ttttctggat aaaccgtgcc
     1201 tgctctttgc cgagagccac gaaaaggtca ggggtatttt ttctgaaaga agcggcaaag
     1261 taccgataga aagggttatt cttgctcaca agtcccgctg aagcaaaggc atcagggtac
     1321 agatccagag accaatagat ccagggcacg ccctttttct tgcagaggtt tccgctccag
     1381 taattcaaga ggggaggatc agtcagcgag attataatat cagccttcgt aagggcggtt
     1441 tttataagct tgcgtccctc aaggaaattt cccaagaacc tcaagagctt gcttctcccc
     1501 ttataaatag gttgtaaggc agagaggttt ccgacaggct ttttgttcga ccctccgccc
     1561 atgtatgggg catcgatata attaacggta acggcaactt cagtaagaca attgagcagg
     1621 tatgcggcca tcttggacgc ctcttcgccg gttaccgcag ggtttggcgg ataataccgg
     1681 tttacaatat ggacgttata cactaccccg ctgtttccaa gatctgaata tttcgaggaa
     1741 tatatcatca agggatttcg taatggacca gtgcgggtaa tgttctttta ttttgctcag
     1801 gttgctgata tagcagatat ggtcgccttc cctgttcctt tctctgtact catagtgcat
     1861 tttttccccg gaaattgtct cgatcttttc aaatgcctca aggatcgaaa tggaattctc
     1921 tctgccgcct cctatattat agactccggc aatcctcggc tcttggtaaa aggcgtagat
     1981 aaactgtgcc acgtcaaagg aatggatatt gtcacgtacc tgtttcccct tgtaaccaaa
     2041 gacgttatat actcttccct cgacattaca cttgatgagg taactcagaa acccatgaag
     2101 ttctaccccg gaatgagaag gaccggtgag acatcctccc ctcaaacaac atgttttcat
     2161 tccaaaatat cttccatatt cctggacaat gatatctgag gcgacttttg acgcaccgaa
     2221 tagagaatgc tttgactgat caatcgtgaa tgtttcatca atgccattgg catagcgggg
     2281 atcatcatag tcccaccggc tgggtagttc ccttaactta atcaagttgg gtgcatcacc
     2341 atagaccttg tttgttgaca tatgcacaaa gaccacgttg ggggaatact gtcgtgctgc
     2401 ctcaagaagg ttcattgtac ccactgcatt cgtatcaaaa tcttctaaag gcctcgatgc
     2461 tgccagatcg tgactcggtt gggctgctgc gtggacaatg agggaaggtc tgagatcctc
     2521 gatgactttc gtgatgccct gtctgtccct gatatcgcat tcaacatggc ggaaattagg
     2581 taccgtggat tctaaacgtc tttgattcca cctcgtgtcc ccggatgggc cgaagaagga
     2641 cgccctcatg ttattatcga ctccatagac cgaatcaaac cctctgtaaa gtaccgaaac
     2701 cacctctgat cctatcaatc ctgacgaacc ggtaaccagc atgattttct tagacattct
     2761 gcattctcct gataatgaat ttatccagga ccagcatatc catcttggtc cttaaaaaac
     2821 aatctaatgc ttcttccggt gtgcatacaa tcggctcatt ctcattaaat gatgtattca
     2881 agacgacagg cacaccggtg attttcctga attcctctat caactgccag taggccgggt
     2941 tatcctcata ccttaccgtc tgcagtctcc ctgtcccgtc aacgtgggtc acggcaggta
     3001 tgatgtcccg cttttccggt ctgattttgt agaccttgag cataaaggga tcggggtaat
     3061 cagtttcaaa atactcaccc acagcttcca gatggatcga gggcgcaaaa ggtctgaagg
     3121 actccctccg cttgatctta agatttaaga tatctttcat gtcaggtctc ctgggatcac
     3181 agacaatact tctgtttccg agcgctctcg ggccccactc catctttccc tggaaccatc
     3241 ccactacctt gccttgtgag atcgcacgtg ccgtctgcag gcagagatct tttatgtctt
     3301 cgattgtttc aaaagcgcag ttttcggaat tgagctcatt gctcttggct gtgagtaggc
     3361 tgctgatgtg agcggtggtg aattcagggc cgagataagg tctttccaaa acaaatgaac
     3421 ggggtttatc caatagctga ttccagacgt aaaaggctgc cccaatcgaa ccgccagcat
     3481 cacctgcggc gggttgaata tagacctccc gaaagggggt gttttgaaaa atctttccgt
     3541 tggccagact attcatagcg cagccgcccg agagtgcaag gcgaggacat cgtgtttttt
     3601 cgtacacatg attcaggata tgaaagaaga cttcttcata tctcctctgc attgaggccg
     3661 caatattttt gtgtctttcc ccaatctcag cttccttctt tctggcgggg cctaagcgtt
     3721 cttcaagttt tcgggagaag accgatccca tatgcggttc accgctttcc caggtcattg
     3781 aaacaccctc ggagtgatgg ataaagaagt cgaggtcaag ttcaaaatct ccgtcccgga
     3841 gcattctgag aatcgtcttc atctcctcca tgaatgcggg ttcaccatac gcagcaagac
     3901 ccatgacttt atattcatcg ccatatttcg ggaatcccag gtattgggtt aaggcaaggt
     3961 aaaagaggcc gagggaatgg gggaaaaagg tacggtgagt aatggtgatt ttgctgcccc
     4021 cggcaatacc ccacatcgcc ccgacaaaat caccaaatcc atctactgat acgacagcag
     4081 cttcttcaaa gggagatacg aagaaggaac ttgcgagatg ggcagcgtgg tgttcaatag
     4141 tatggagttc tgccttaagt ttcgaacgag gaaccttgaa tgttgaactc agaacagttc
     4201 tcaggtcagt tatccttgag gcattcttaa ggcggtccga gaggatacgt gaagagggtc
     4261 tcttgaaaaa agcgaaaagc acttttttcg ccagattcgc ctttggatca cggttaatgg
     4321 cgatatggtc gacttctccg atatcgcttc ctgtctcctt gaggcagtac tgaatcgcct
     4381 ggaccggcaa ccctgcccag tgttttatcc gcgtaaatcg ctcctcttca actgccgcaa
     4441 taagcttgcc gtcaacaaca atgcatgcgg agctgtctcc atgatatgca ttcaatccaa
     4501 gtattatcat ttacttttca cgattcacgg gttcataagc gttaaggccg aggataatca
     4561 tattctcact ctgattgaag aacgtggagt cacttccatc gccatgtttc cagtcctcca
     4621 ggcttgcaga aaccgattgt ccagctatct cctttaaggc tgttcagctg gaacttcata
     4681 ccagccccgg caaaggcaac gtcctgtgga gggttaaact gaaaacagag atcccaccgt
     4741 gcacgcggaa acgcaagctc tgcggccgat acactcatta ttgttccatt ttgttgagca
     4801 tcactatcct ttccttgcct atctcctctc atgatcccgc tccattgagg gggaccttcc
     4861 ggagagaccc atatccccgg cagggtaaag aatgtcttct caaattgcgt ccagttcgga
     4921 tccatcttca ccatcaggcg gtctccgaag aagagaacct gatagcgggc ccgttcctct
     4981 acactcgcag tgagcaaagc cggagtttca accggccagg taaaggcggt cttggtttta
     5041 tcggacagta attcctgatt tccgtcagaa aaggtaaaga gcgggttgcc atccaatctt
     5101 atggtgtcat catcatcggc gagatacctt gagaggccat tgaacatatc caattttacc
     5161 gtgtaccttg gtgcatggat ctggtaaaca gccggttgcg gagattgata gacagattca
     5221 agcagggcat taatggcaac aacaattgat ttggcagcag tgcggatgga ctttccctct
     5281 ccggcctgac ggtaatgaat gcgatacggg accacatgac ggccttcatt cggattatta
     5341 gaacggaatg ttactattac ctcagcggtg tctcccggtt tgagagggcc gaattcaggc
     5401 tttgaaccgt ctattaccaa tccttttgga acctcaaatt cgagccagcc tgaaacagac
     5461 tctttaagga cattcctgat tctatacgtg atcttccgtg tatcccgggg aaacatgcgg
     5521 accacctcag gaaagaattt aaccggaaat ggacattcag gatcaacaag caacggagga
     5581 tccgctttcc ctggtaacga accaaaaaca agataaccat tatcactttt tgcagccgcc
     5641 ggctgtccat ttactgtaag aaggccttga gcgtgaggaa ggcgaaaacc gatccacccc
     5701 ccatgagcca gtatcctctc attaccggca acgcgaataa
//
LOCUS       DATKOU010000079         5164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4720141, whole genome shotgun sequence.
ACCESSION   DATKOU010000079 DATKOU010000000
VERSION     DATKOU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4720141"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VMU21_03770"
     CDS             complement(<1..353)
                     /locus_tag="VMU21_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76674.1"
                     /translation="MQRGTGGKEFMKILKMIALAICLAMALTTSSLAKETVKIGAILA
                     VTGPASNLGAPEARTLQMLVEDINGKGGIDGKMIELLLKDSGSSPERAVSFAKQLIEE
                     EKVLAIIGPSTSGET"
     gene            complement(413..1561)
                     /locus_tag="VMU21_03775"
     CDS             complement(413..1561)
                     /locus_tag="VMU21_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO76675.1"
                     /translation="MKVAKFVSAVVLLVALTIAPAFGAETIKVGAILAITGPASFLGA
                     PEARTLEMMVEEINAKGGIKGKKVELIIKDSGASPEKALSFAKQLIEEDKVLAIIGPS
                     TSGETMKIKNVAEEGKTILLSCAAAEVIVNPVAKYVFKTPQKDSYAVIKIFEQMKKMK
                     LSKVGVLSSNTGFGKAGKDQIEKLAPEHGIEIVANEVYDKAATDLTAEVTKVKASGAE
                     AIINWSIEPAQSIVIKNARQIGFKGQIFQSHGFGNIKYVEAAGPAAEGVIFPAGRLLV
                     AEVLPKNDPQKAVLMKYKNDYELKCHGEECSTFGGHAYDAMLILIKAIEKANSTDKEA
                     VRSAVEGLKGVVGTAGIFNFSPEDHNGLDMTSFVMLTVKKGKFALLGK"
     gene            complement(1589..2020)
                     /locus_tag="VMU21_03780"
     CDS             complement(1589..2020)
                     /locus_tag="VMU21_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HUO76676.1"
                     /translation="MKVEQISIFLENKSGRLAEVTELLAKSEINIRALSLADTADFGI
                     LRLILNDSEKAKKVLKENGFTVGKTEVIAVEVPDRPGGLAGILNALQGEGINVEYMYA
                     FVQKSGENAIIIFRFDELEKAIAALQKAGVRILKGEEVYAL"
     gene            complement(2131..3432)
                     /locus_tag="VMU21_03785"
     CDS             complement(2131..3432)
                     /locus_tag="VMU21_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HUO76677.1"
                     /translation="MIWNEKMECMSRKEMKAIQSERLKKIVSYVYKNCPLYRTKFDVI
                     GVKPSDIKGIGDIHKLPFTVKDDMRDSYPFGLFSAPMGKIKEIHVSSGTTGNPTVVGY
                     TKEDIKLWSEVMARAISCAGAEPGDKIQIGYGYGLFTGGLGFHYGALEMGLTIIPASS
                     GQTKRQLKLMNDFKPRILACTPSYALYMADEAREMGLDPRDSSWEIGIFGAEPWSESM
                     RKEIEATWNMLATDVYGLSEIIGPGVAQECTHKIGLHIWWDVFYPEVVHPETGKVRDE
                     GEVGELVITTLTKKGIPLIRYRTRDNVSITYEKCSCGRTTPRISKIKGRTDDMIVVRG
                     INVFPSQIEHVLLSIEGTQPHYQLIVDRKVGELDELEILVEVEEKIFSDEIKKMQMLG
                     DKIKREISSILGISAKVKLVEPKTIERSEGKAKRVVDKRNL"
     gene            complement(3472..4074)
                     /locus_tag="VMU21_03790"
     CDS             complement(3472..4074)
                     /locus_tag="VMU21_03790"
                     /inference="COORDINATES: protein motif:HMM:NF005325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HUO76678.1"
                     /translation="MRDKTTNILLCGVGGQGILLASEIISSALMSAGYDVKQSEVHGM
                     AQRGGSVVAHLRYGAKVYSPLIELGGADFAVSFELIEALRYLPYYNRESKVIINTQKI
                     LPAPVSAGVETYPSDILDQLVSRRLAVFPMDAFELARSAGEARAVNMVLVGALSVFLP
                     VKEEILLEVIEGRIPEKVRKVNTEAFVRGREKVGVLQGKK"
     gene            complement(4064..>5164)
                     /locus_tag="VMU21_03795"
     CDS             complement(4064..>5164)
                     /locus_tag="VMU21_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUO76679.1"
                     /translation="SGVSYLYVKEAFPEASLLKLGMGYPFPEKLIRGFAKEVKELFIV
                     EELDPFIEFHVKAMGIPCRGKEVIPSMGELNPRIVRAAVMGGKTKAIFEPVLIPMRPP
                     NLCPGCPHRGIFYALSKLGLFVAGDIGCYTLAALRPLSGMDSCVCMGAGIGTAFGMEK
                     ALGKEALGKIVAVIGDSTFFHSGITGLIDIVYNRGFTTVIILDNRTTGMTGHQPHPSS
                     GMTVRGDFTTAIDIGALCRAIGVQHVMSVNPNDIEAAQKIVKQEIERPEPSVIIAKSP
                     CVLLPEMKKRKDKKLYIVIPEKCTGCRTCLKLGCPAIEWVPLTPDEAKKLGYKESQKG
                     YSRINAVLCDGCSQCAPLCKFKAIVVAGKDEG"
BASE COUNT         1124 a         1514 c         1142 g         1384 t
ORIGIN      
        1 atcgtctccc cgctggtgga aggcccgata atggcaagaa ccttctcctc ctctataagc
       61 tgctttgcaa aggagacagc cctctcaggg ctgcttccgg aatccttgag gagaagctct
      121 atcatctttc cgtcgatgcc gccttttccg ttgatgtctt ccaccagcat ttgcagcgtc
      181 cgtgcttctg gtgcgccaag atttgacgct ggtccggtca cggccaggat cgcccctatc
      241 ttgacagttt ccttcgccag cgacgatgtt gtcaaagcca tcgcgagaca gatcgcgagg
      301 gcgatcatct tcagtatctt catgaactct tttcctcctg tacctctctg cacgcagact
      361 cctggcatgc ctggatcata tacaggtccg ccggtcagag cgcttcatac acctatttcc
      421 cgagcagcgc aaacttgcct ttcttgacag ttagcatcac aaaggacgtc atgtccagac
      481 cgttgtggtc ttctggagag aagttgaaga tgcctgcagt accgactacg cccttgagcc
      541 cttcgaccgc cgatctcacg gcttctttgt ccgtactgtt ggccttctcg atggccttta
      601 tgaggataag catcgcgtca taggcatggc cgccgaaagt gctacattcc tccccatggc
      661 atttcagctc gtagtcgttc ttgtatttca tcaggacggc cttctgggga tcgttcttgg
      721 ggagcacctc tgccactagc agccgtcccg cggggaaaat gacaccttcg gctgccgggc
      781 ctgctgcctc gacatacttg atgttgccga atccgtggct ctggaatatt tgacccttaa
      841 agccaatctg acgggcgttc ttgatcacaa tggactgggc cggctcgatc gaccagttaa
      901 tgatcgcctc ggccccggat gccttcacct ttgttacctc tgcagtgaga tcggtcgctg
      961 ccttgtcata cacctcattg gccacgatct cgatgccatg ctcaggagca agtttctcaa
     1021 tctggtcctt gccggccttg ccgaaaccag tgttgctcga gagtaccccc accttcgaga
     1081 gcttcatctt cttcatctgc tcgaatatct tgatcacggc atagctatcc ttctggggtg
     1141 tcttgaagac gtattttgcc acaggattga cgatcacctc tgctgccgcg caggaaagca
     1201 ggatcgtctt gccctcttcc gcaacgtttt ttatcttcat cgtctcgccg ctggttgaag
     1261 gacctatgat agccagcact ttgtcttcct caatgagctg tttggcaaaa gagagcgcct
     1321 tctcagggct tgcgcccgaa tccttgatga tcaactcaac ctttttgccc ttgataccac
     1381 ccttcgcatt gatttcttcg accatcatct cgagcgtcct tgcctccggg gcgcctagga
     1441 acgaggccgg accagtaatg gcaagtattg cgccgacctt gatcgtttcc gccccgaatg
     1501 ccggagcgat ggtgagcgcg acgagcagaa ctaccgctga aacaaatttt gctaccttca
     1561 tttccattct ctccttttca ctgatagatt ataacgcata tacctcttcg cccttgagta
     1621 tcctgacacc cgctttctgg agtgcggcga tagctttttc cagctcgtca aagcggaata
     1681 tgataatggc attctcaccg cttttctgga caaaggcata catatactcc acgttgattc
     1741 cttccccttg cagcgcattg agaatgcctg ccagccctcc aggtctgtca ggaacctcaa
     1801 cggcaataac ctcggtcttc cccacagtga agccattctc cttcagcacc ttttttgcct
     1861 tctcagaatc attcaggatg agcctcaata tcccgaaatc tgccgtgtcc gccagggaga
     1921 gggccctgat atttatttca gattttgcca acaactccgt cacctcggca agtcttccgg
     1981 acttgttctc gaggaatatg gatatctgtt caaccttcat atatgcctcc tattcgagtt
     2041 atcagctttt agcaatcggc cctcagccga gaataataat gaaaagaatc ctgccgagtt
     2101 gaacgctcac agccgtttgc tgaccgctta ttataaattc ctcttatcca caacccgctt
     2161 tgcctttccc tcgcttctct caatggtctt gggctccacc agcttcacct tagcagaaat
     2221 gccgaggatc gaacttatct cccgcttaat tttatcccca agcatctgca tcttcttgat
     2281 ctcgtccgag aagatcttct cctccacctc cacaagaatc tcaagttcgt ccaattcccc
     2341 tacttttctg tccacaatga gctgatagtg gggctgcgtc ccctctatgc tcaggagcac
     2401 atgctctatc tgggacggga agacattaat gcccctcacc acaatcatgt catcggtcct
     2461 tcctttaatt ttacttatcc tcggagtagt tctgccgcag ctacatttct cataggtgat
     2521 gctcacatta tcccttgtac ggtagcgtat gaggggaatc cctttcttcg tgagcgtcgt
     2581 gatcaccagt tcccccacct ccccttcatc cctcaccttc cctgtttcag gatggacgac
     2641 ttccggataa aagacatccc accatatatg gaggcctatt ttatgcgtgc actcctgggc
     2701 cactccaggc ccgatgatct cggaaaggcc gtacacatca gtggcgagca tattccaggt
     2761 cgcctcaatc tccttcctca tagattcgct ccagggttcg gcgccaaaaa taccaatctc
     2821 ccagctgctg tcccgtgggt caagccccat ctctctggct tcgtcggcca tatagagtgc
     2881 ataacttggt gtacaggcca ggatccttgg tttgaaatca ttcatgagct tcagctgacg
     2941 ttttgtctgg cctgaagatg cgggaatgat cgtcagtccc atttccagag caccgtaatg
     3001 aaatcccaat ccaccggtaa agaggccgta tccatagccg atctgaatct tgtcaccggg
     3061 ttcggcgcct gcgcaggaga tggcccttgc cattacctct gaccagagtt taatatcctc
     3121 cttggtgtat cccacaactg tgggatttcc cgtggtgccg ctggagacat gaatttcctt
     3181 gatcttgccc atgggcgcgg aaaaaagacc aaagggataa ctgtctctca tatcatcctt
     3241 tacagtaaag ggcagcttgt gtatatctcc aatacccttg atgtctgagg gtttcacccc
     3301 tattacgtcg aacttggtcc tatagagggg acaattttta tagacatagg agacaatctt
     3361 cttcagcctc tctgactgaa tagccttcat ctccttacgg ctcatgcatt ccattttttc
     3421 attccatatc atgactccag cctcacctcc tcaaaagagt cgtaactatc ttcatttctt
     3481 tccctgaagc acacccactt tttctcttcc cctgacaaag gcttctgtgt tcaccttcct
     3541 gactttctca ggaatccttc cttcgattac ctcaagaagt atctcctctt tcacaggaag
     3601 gaagacagag agtgctccca ctagcaccat gtttaccgcc cttgcctctc ctgcagaccg
     3661 cgccaactca aaggcatcca tcggaaatac ggctagccgt ctgctcacaa gctgatcaag
     3721 gatatcgctg gggtaggttt ccacacctgc agagaccgga gcaggcagta tcttctgggt
     3781 atttataata accttgctct ccctgttgta gtatggcaaa tagcggaggg cctcaataag
     3841 ctcaaatgaa accgcaaagt cggctccgcc caattcaatg agcggtgagt agaccttggc
     3901 tccataacgg aggtgcgcca caacagagcc tcccctctgg gccatgccgt gcacctcgct
     3961 ctgcttcaca tcatatcccg cactcataag cgctgatgaa atgatctcgc tggccaggag
     4021 tattccctga ccgcccacac cgcagaggag gatattcgtt gtcttatccc tcatcttttc
     4081 ctgccaccac aatggccttg aacttgcaca gaggggcaca ttggctgcag ccgtcgcaca
     4141 aaactgcgtt tatccttgag tatcccttct ggctctcctt gtacccaagt tttttcgcct
     4201 catcaggggt aaggggtacc cactctattg caggacagcc aagcttgaga caggtcctgc
     4261 agccagtgca tttctcaggt atcacgatat agagcttttt gtccttccgc ttcttcattt
     4321 ccggcaggag gacacaagga gacttcgcga taataacaga tggctcgggg cgctcgattt
     4381 cctgcttcac aatcttttgt gccgcctcga tatcattggg gttcacgctc atcacatgct
     4441 gaactccgat tgctctgcaa agcgccccaa tatcaatggc tgtggtaaaa tctcccctga
     4501 cggtcatacc agaggaggga tggggctggt ggccggtcat gccggtggtc ctgttatcaa
     4561 gaatgattac tgtggtaaat cccctgttat agacaatgtc aatcagtcct gtaatgcctg
     4621 aatggaaaaa ggtggaatcc ccgatcaccg ccacaatctt tcccagcgcc tctttaccca
     4681 gtgccttttc catgccgaag gcggttccga tgccggcccc catgcatacg catgagtcca
     4741 tgcctgagag tggtctgagc gctgcgaggg tataacagcc tatgtcgcct gccacaaaga
     4801 gaccgagttt tgaaagagca taaaatattc ccctgtgggg acagccaggg caaaggttcg
     4861 gcggcctcat gggaatcagc acaggctcaa aaatagcttt tgtctttcca cccatgaccg
     4921 ctgcccgcac gatgcgtggg ttcagttctc ccattgaggg tatcacttct tttcccctgc
     4981 agggaatgcc catagccttc acatggaatt cgataaaggg atcaagttcc tccacgataa
     5041 acagttcctt aacctctttt gcaaaacccc ttatcagctt ttcaggaaag gggtaaccca
     5101 taccgagttt gaggagagag gcctcaggga aagcctcttt cacatacagg taggagacgc
     5161 ctga
//
LOCUS       DATKOU010000080         4005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5101157, whole genome shotgun sequence.
ACCESSION   DATKOU010000080 DATKOU010000000
VERSION     DATKOU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5101157"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VMU21_03800"
     CDS             <1..737
                     /locus_tag="VMU21_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUO76680.1"
                     /translation="WWFWVWVFLFLLSIFMMYLSPYVIEPLFNKFTPIEDEALKGRIM
                     AITEKAGIHVSRILRIDASKRSRHTNAYFTGIGKTKRIVLYDTLLEGMAHNEILTVLA
                     HEIGHWKRHHLLKTMLILEAASLAALYLCSRLIQSDLPTTLFHISEETVFAKLALLGF
                     LGGIIAFPLKPLMNSLMRRHEREADNASYELTKDAESMVSAFVKLSRENLSNLHPHPL
                     YVALHYSHPPILERIQSIRNMDNKEK"
     gene            complement(754..1821)
                     /gene="mqnE"
                     /locus_tag="VMU21_03805"
     CDS             complement(754..1821)
                     /gene="mqnE"
                     /locus_tag="VMU21_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HUO76681.1"
                     /translation="MQQRVSERVLSGARITGEDAIALFQSDDLFTLGRLASHMTEKRH
                     GKNAYFVVNRHINPTNICVNRCRFCAFSRSKGEKGAFALTVDEILSKLGEAAKGGHPI
                     REVHIVGGLHPDWPFDYYIEMIAAIRKKFPSLHIKAFTAVEIDYFSRISGLSVREVIE
                     RLKDKGLGAMPGGGAEIFAEEVRERLCPEKISGQRWLDIHKTAHLCGIPTNATMLYGH
                     IENYEDRVDHLLRLRQLQDITGGFQAFIPLAYHPKNTEMGGSYSSGIDDLKTTALSRM
                     ILDNIPHIKAYWIMLGEKIAQLALLFGADDLDGTIIEEKITHSAGAISSEGLTRDELR
                     NLITKAGKRPVERDAFYHVVG"
     gene            1986..>4005
                     /locus_tag="VMU21_03810"
     CDS             1986..>4005
                     /locus_tag="VMU21_03810"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76682.1"
                     /translation="MNELIIEGARQNNLRNFTLTLPHNKVIAITGVSGSGKSSLAFDT
                     IFAEGQWRFIESISTYARLFLEKLDRPDVDAIQNIRPAVALEQKNPVRGSRSTVGTLT
                     EIYDYFRLLYSKVSTPYCPACGKEIRKWDTSEVVCLLKEEYASKKALIIFHSGEQLEA
                     LKKRGYRRLLIHGEVVDMDELGDIKMTRQEVASHVDVVLDRIVIRDEPRLSDSVEMAW
                     KEGNEKMKVMIMEDAANPAVVLTFTSANACDGCGKELPEPSPLLFSFNHPVGACQNCK
                     GFGNILTYDRDLIVPDVYLSLSEGAIEPWEKPAARWWKRQFIAGAKKAGIDVKKAYRE
                     LSQDEKEMIFRGGKDFYGIDDFFEDLEGKRYKLHVRVFLSRYRKGSVCPVCHGKRLQA
                     DALSYKISHCDIADLSDMPVSKLITFFRDIDLSPFQKDLAKEILRQIQGKLSFLHRVG
                     LDYLTLSRQGKTLSGGEYQRVNLSTQLASYLTGTLYVLDEPTVGLHARDTERVAEIMA
                     ELSKMGNTIIVVEHDKGIIDSADWVVELGPGGGQQGGEVVFSGTKKQFLQADTLTSRY
                     VKGVDRITRSHGSGRTSAGHELILQNARGNNLKGINLRIPLRTFTAVTGVSGSGKSSL
                     VVETLYIALAKLFGLENESPLPYDAIQGVKLIKGLKIIDQTPIGRSPRS"
BASE COUNT          987 a          995 c         1034 g          989 t
ORIGIN      
        1 actggtggtt ctgggtatgg gttttcctgt tcctcctcag catttttatg atgtacctgt
       61 ctccctatgt gatcgagccg ctttttaata agttcacacc gattgaggat gaagcgctga
      121 aggggaggat tatggcgata acggaaaagg cgggtatcca tgtgagcaga atcctgagga
      181 tagatgcgtc aaagaggagc aggcatacca acgcctattt caccggaata ggaaagacaa
      241 agcggattgt actctacgac accctccttg aagggatggc ccataatgag attctcactg
      301 tgctggcgca tgagattgga cactggaaaa ggcatcacct gttaaagacc atgctgatcc
      361 ttgaggcggc ttcccttgcg gccctgtatc tttgctccag gcttatccaa agcgatcttc
      421 ccactacact ctttcatata agtgaggaga ccgtttttgc caaactggca ctccttggat
      481 tcctcggtgg tatcattgct tttcccctga aaccacttat gaactccctc atgaggaggc
      541 atgagaggga ggctgacaac gcttcctatg aacttacgaa ggatgcagaa agcatggtga
      601 gtgcctttgt gaagctttca agggagaacc tgtcgaacct ccatccccat cccctctatg
      661 ttgccctcca ctattcccat cctcccattc tggagaggat acagtccatc aggaatatgg
      721 acaataaaga gaaatagagg ggtttcccgg acattaaccc acgacatggt agaaggcgtc
      781 tctctccacc ggtcttttgc ccgcttttgt gataaggttc ctcagttcgt cccgcgttaa
      841 tccctcgctg ctaattgcac ctgctgaatg ggttatcttt tcctcgatga tggttccatc
      901 caggtcatcc gcgccaaaga gcagggccag ttgggcaatt ttctctccaa gcattatcca
      961 atacgccttg atatgcggta tgttatcgag aatcatcctg ctgagggcag tggtcttcag
     1021 gtcatcaatt cccgatgagt atgagccgcc catctccgtg ttcttcgggt ggtaagcgag
     1081 ggggataaat gcttggaaac cgccggtgat gtcttgcaat tgccttaatc tcaggagatg
     1141 gtcaaccctg tcttcataat tttcaatatg gccataaagc attgtcgcgt ttgtagggat
     1201 tccgcagagg tgggctgttt tgtggatatc gagccatctc tggcctgata tcttctcagg
     1261 acagagcctc tccctcacct cttctgcgaa tatctcagcc cccccgcccg gcattgcccc
     1321 aagtcctttg tcctttaacc tttcgatgac ctctcttaca gaaaggccgc tgatccttga
     1381 gaaatagtct atctccactg cggtgaacgc ctttatatgg agggaaggga attttttcct
     1441 gatggcggcg atcatctcaa tataatagtc aaagggccag tcgggatgga gcccccccac
     1501 gatatgaacc tcacgaatgg gatgcccccc tttcgctgcc tctcccagtt ttgaaaggat
     1561 ttcatccacg gtcaacgcaa aagccccctt ctcacctttt gaacggctga aggcacagaa
     1621 tctgcatctg ttcacacaga tattggtagg gttaatgtgg cggttcacga caaaatacgc
     1681 gttcttgccg tgccttttct ctgtcatgtg ggacgcgagc ctgccgaggg tgaagaggtc
     1741 atcactctgg aataacgcaa tggcgtcttc cccggtgatc ctcgctccgg aaaggactct
     1801 ttcgctcaca cgctgctgca ttattcatta taaaacaaat ggtaattaca tttcttctga
     1861 tatggcccag gggaatgcat ctgtcagcgc caggacacca ttgaaaacga tcaaggcacc
     1921 gaccatcgca cttaagtcag caagcgcctg tatccacacc aaaatgttaa aatatcagga
     1981 tgtcggtgaa cgaattgata attgaggggg cgcggcagaa caatctcagg aacttcacac
     2041 tcacattgcc ccataacaag gtcatcgcta ttacaggggt atctggttcc ggcaagtcgt
     2101 ccctcgcctt tgataccatc ttcgcggagg gccaatggag gtttattgaa tccatatcca
     2161 cctatgcaag gcttttcctt gaaaagcttg acaggcctga tgtggatgcc attcagaaca
     2221 taaggcctgc agttgccctt gagcagaaaa atcccgtgag gggttcccgc tccactgtgg
     2281 ggacgctcac cgagatctat gactatttca ggcttttata ttcaaaggtc tcaacgcctt
     2341 actgcccggc gtgcggcaaa gagatacgga aatgggacac ttccgaggtt gtgtgtctct
     2401 taaaagaaga gtatgcgtcc aaaaaggcgc tcattatttt ccactccgga gagcaactgg
     2461 aagccctcaa aaaaagagga taccgccgcc tcctcattca cggtgaggtg gtggacatgg
     2521 atgaactcgg tgatatcaag atgacccggc aggaagtcgc atcccatgtt gatgtggtcc
     2581 tggacaggat tgtgataagg gatgaaccga ggctttctga ttctgtggag atggcctgga
     2641 aagagggaaa cgagaagatg aaggtgatga ttatggagga tgccgcgaat ccggcggtgg
     2701 tactcacatt cacctctgcc aatgcatgtg acgggtgcgg caaggaactc cctgagccct
     2761 ctccccttct gttttccttt aaccatcctg tgggcgcatg ccagaactgc aagggattcg
     2821 gaaatatttt gacctacgac agggatctca ttgtccctga cgtgtacctc tcactgtcag
     2881 agggggccat agagccatgg gagaagccgg cagcacgatg gtggaaacgg caattcattg
     2941 caggggcaaa gaaggcaggc attgatgtga agaaggcgta cagggagctt tctcaggacg
     3001 aaaaggagat gatattcagg ggaggcaagg acttctatgg tattgatgac tttttcgaag
     3061 acctggaagg caagcgatat aagctccatg tgagggtgtt tctcagcaga tacagaaaag
     3121 gctctgtatg tccggtatgc cacgggaagc ggcttcaggc ggatgcactc tcctataaga
     3181 tttcccactg tgacatcgca gatctctccg atatgcctgt gtcaaagctc atcacattct
     3241 ttagggatat tgatctttct ccatttcaaa aagacctggc caaggaaatt ctaaggcaga
     3301 tacagggaaa actctcgttc cttcaccggg ttgggttgga ttatctcacc ctgagcaggc
     3361 aggggaagac cctttccggg ggagaatacc agagggtgaa cctttcaacc cagctcgcgt
     3421 cgtatcttac cggtaccctc tacgtgttgg atgaacccac cgtgggactc catgccagag
     3481 acactgaaag ggtcgccgag ataatggccg aactctcgaa gatggggaat accatcatcg
     3541 tggtggagca cgataaagga atcattgatt cggccgactg ggttgtggaa ctcggcccgg
     3601 gtggtggcca gcagggtgga gaggtcgtat tttcagggac aaagaagcaa ttcctccagg
     3661 ctgacaccct cacctccagg tatgtgaaag gggtggacag aataacacgt tctcatgggt
     3721 caggacgcac ttccgcaggc catgagctta tactccagaa tgcgaggggc aataatctca
     3781 agggtattaa tctgaggatt cctctccgga cattcaccgc ggtgacaggg gtatccgggt
     3841 caggaaagag tagtcttgtg gtggagaccc tctatatcgc cctggcaaaa cttttcgggc
     3901 tcgaaaatga gagccctctc ccctatgatg caatacaggg tgtcaaactg ataaaagggc
     3961 tgaagatcat tgatcagacg cctatcggca ggtctccccg ctcca
//
LOCUS       DATKOU010000081         9596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3179995, whole genome shotgun sequence.
ACCESSION   DATKOU010000081 DATKOU010000000
VERSION     DATKOU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3179995"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VMU21_03815"
     CDS             <1..188
                     /locus_tag="VMU21_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76683.1"
                     /translation="TGSDTKKPYKNILTGINKEEGAILCLEQIVTTDFAKKRCFLTAL
                     ALQERPGMASMILEHVP"
     gene            241..576
                     /gene="yajC"
                     /locus_tag="VMU21_03820"
     CDS             241..576
                     /gene="yajC"
                     /locus_tag="VMU21_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HUO76684.1"
                     /translation="MFIDIAYAMGPAPQAGQAGGASSMLTSLLPLVLIFVIFYFLLIR
                     PQQKRAKEHRQMLDNIKKGDKVITSGGIYGVVESVGTNTLTLKIAENVKVKFGKGNIA
                     AVRPPSEED"
     gene            727..2349
                     /gene="secD"
                     /locus_tag="VMU21_03825"
     CDS             727..2349
                     /gene="secD"
                     /locus_tag="VMU21_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HUO76685.1"
                     /translation="MKKNIIWRFVLIGITVVLAIVFFLPNMPLFQYMPEWWKNTMPNK
                     GITLGLDLQGGVHLVFEVEGDRAVEITTERIAQSLKDMLGRKKLTAEVSRNGLLITVS
                     PNSMEVRKAVEESYPILTPTETGTKLVYKLSDREAERIKDNAANQALETIRNRIDQFG
                     VAEPTIHRQAANEIVVQLPGIKDPKRAIEIIGKTAQLEFKMVDDSSPVAAEIPASVRA
                     GEEDVLLAKFSGKIPADDEILFERVVNRETGEVTKRPVLLKKQTMMTGDLLTEAKVNI
                     DQRFNEPYVSLTFNAAGAKLFEEITGANVKKRMAIILDGTVYSAPVIQERISGGNAQI
                     TGRFSMDEARDLAIVLRAGALPAPIKMLQNVTVGPSLGSDSIEAGKRAGIIGTILVVV
                     FMIVYYRLSGVIADFALLLNIILLLGAMASLNATLTMPGIAGIILAIGMAVDSNVLMF
                     ERIRDELKAGKTPRASVDSGYDKAFWTIFDSHVTTLITAAVLFQFGTGPIKGFAVTLS
                     LGVAINLFTALVGTKSTFDLLNSRQEVKKLSI"
     gene            2358..3269
                     /gene="secF"
                     /locus_tag="VMU21_03830"
     CDS             2358..3269
                     /gene="secF"
                     /locus_tag="VMU21_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HUO76686.1"
                     /translation="MLELVKDTKINFLGKRKFAFIFSGILSLVGIFTIVQIANGKANL
                     GIDFAGGTAIQLKFEKPINLHEVRKALEDGGIRDFDLQDLPAVQKILIREKKTVQQLG
                     QVSDTITGIISQKFPDNKYVVDSTTLIGPKVGGKLRQDAAQAIVLATLGILIYIAIRF
                     KFDFAVGATVATFHDVLAVLAIFFLLGREINLILVTALLTIAGYSLTDTVVVFDRIRE
                     NLRTRLKEPVETVMNSSINEVLSRTIITSLTVLITSFALFLFGGEVIHDFSLAMILGV
                     IIGTYSSIFVASPIVLLWRKKRPFAKK"
     gene            3336..5135
                     /locus_tag="VMU21_03835"
     CDS             3336..5135
                     /locus_tag="VMU21_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HUO76687.1"
                     /translation="MVSSRPYMNRRWFISRANPEYVRYLSKAASISPILTQVLLKRGF
                     KTPGDITDFLTPGLAQLTDPYELPHMKAAVERIKGALKSNERVLVHGDYDTDGLTAAS
                     IMVHALRVMGLDARYFIPNRMIHGYGFNQPGVDEAKKTGVKLIITVDCGITSFAAAAY
                     AKKEGIDVIITDHHEPNRRVQESNSRSMVSGQQPEAKDEFLLPDATVIVNPKLGKNPG
                     LSVLSGAGIAFKMVQAFAMDDALPVTADDSLSLLDLAALGTLADVVPLTGENRIILKE
                     GLRSLQHSHRPGIRSLLEVSGLKEREVRSGLLSFTLVPRINASGRMGDASDVMRLFLS
                     DSEGETLSIAERLDMINAERQRIEEEVYQEALSQLNRKGYDAAIVLYDKGWHAGVTGI
                     VASRVAEEFFRPTFVFSVEDTTAKGSVRSIPPFDVYRGLSACSDLLLSFGGHRQAAGV
                     KLNVSHMPLFEERMNTIVRASLSDEDFIRTVEIDAEVFLLEVTNNLIKEFSVLEPLGF
                     GNPEPLLGAKELAFLNPRVVGKNHLKMTLRQTQGKQRSLSMDAIGFSMGKYLLHLDTA
                     TTLDAAFTPMINEWNGNKYLQLHLKALRPSPSL"
     gene            5215..5748
                     /locus_tag="VMU21_03840"
     CDS             5215..5748
                     /locus_tag="VMU21_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HUO76688.1"
                     /translation="MKKWTIDPDHTVATFSVRHMMVANVHGQFNKISGTIHFDPADIP
                     HSSVEVSIDAAAIYTGIPKRDEHLRSEDFLDAGNHPSITFKSTKAEGTGSNRFRVTGD
                     LTIRGITRQITFEAEHFGPVKDPFEESGRSIGFVASATINREEYGMTWNVAMESGGVM
                     VGKEVTITLEVEADLFT"
     gene            5962..7773
                     /locus_tag="VMU21_03845"
     CDS             5962..7773
                     /locus_tag="VMU21_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUO76689.1"
                     /translation="MAFTGDLELLNIVDIIQLLNTTRKSGTLSVRGLKGESRIIFSNG
                     YIVGASHLNNRVRIGTVLVKMNAISPEYLQQALEVQKQAGKDRKPLIATLLELGKLKR
                     DEAAMGLKKLIEMTLVEVIGWTEGTFTLDTEAIAVSPESSYPISRMEQEIGLDAQVVL
                     MDALRILDERERDRQSGKAVPSDEEVFADVISSEGPGAAGEESSITADDLGLGDLDHL
                     ERKMPQYIQVDEVFDPIEVHRQKIRQTLAAFPAEGQEAFVTFLEKSSRNRGASDKSQR
                     QAGRTRALVLFSEDELIKHSVMTLCKDEGILVFATDGEEELDRIVDQCLRIKVFPVLA
                     FDTPGTTERLLSKEKIVSLRQQLKERYPQVPIIQMASPPDRLFTLQSYRDGIRAVFPK
                     PSQDVSKDTFIADTITFLETFRSYVRRLFLEQKDLTAEDSKLVTLKDRIVALRSLDDP
                     SAVSVALLESVSEVYERAVIFIVRQQELVGDKAIGVYAEREEGATSATGLRIPLTAPS
                     LFRNAVEKGQFFCGESDDDVLKKSLFEEIGTPLRASIILIPMRSHGKIVTLTYGDFGD
                     KEAAPVQGEVLEILAQSAGQMVENILYRKLLSKASQK"
     gene            7827..8894
                     /locus_tag="VMU21_03850"
     CDS             7827..8894
                     /locus_tag="VMU21_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUO76690.1"
                     /translation="MDIATFTQILEIAFQKKVSDIHFEVDNPPIFRARGHLIYSKLSK
                     LTWQDTQFIASTVMEQHGRKLPERLTEFDTSYTLSNVGRFRVSVFRQRGNIGLVMRVI
                     PHYIGTFEELNLPTVLGKITEAPNGLILVCGPTGNGKSTTLASMIRYINDHFNYNIVT
                     IEDPIEFIFHSNKSCIIQREVGIDTESFDKALKAAMRMDPDVIMVGEMRDLETIDACI
                     KAAETGHLVLSTLHTHSAASTINRVVGYFPTQVQENIRHRLAESLVATISLRLVRGKG
                     GESILPALEVMYATSTIKACIRDGHLDEIEQYIEKGRDEYQMQTLDQHLIQLVRQEAI
                     ALDEAKRISRSTDLETKLIYY"
     gene            9061..9411
                     /locus_tag="VMU21_03855"
     CDS             9061..9411
                     /locus_tag="VMU21_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HUO76691.1"
                     /translation="MEPSARRHIGGQGKKIEVDADGNLKSAEDWCEELAIYLSEEDGI
                     KLSKDHWEVINFVRWYYLTYQNCPHPKFIVKNLNKKFGDGKYTVKSLYDLFKKQPVMK
                     ACKHAGIPFTAGCT"
BASE COUNT         2550 a         2349 c         2466 g         2231 t
ORIGIN      
        1 ctacgggaag tgacactaaa aagccatata aaaatatctt gactggaata aacaaggaag
       61 aaggtgcaat cttgtgcttg gaacaaatcg taaccacaga tttcgcgaag aaaaggtgtt
      121 ttttgacagc tttagcgtta caggagcgcc caggaatggc aagcatgatt ttggaacatg
      181 taccttgatt aaatgtgaag aaatgtttag tatattagac aaattttttg gaggtgtagc
      241 atgtttatag atattgcata tgcgatggga cctgctccac aggcaggaca ggcaggtggt
      301 gccagttcaa tgctcacaag tctgctcccc ttagtcctga tattcgtcat tttctatttt
      361 ctccttatca ggccccaaca gaaaagggca aaggagcacc ggcagatgct ggataacatc
      421 aagaagggag acaaggtgat cacctcgggt ggcatttacg gagtggtaga gagtgtgggc
      481 accaatacgc tgacgctgaa gattgccgaa aatgtgaagg ttaaatttgg gaagggaaat
      541 attgcagctg tcagacctcc gtcagaggaa gattgagacc aatacacata tttcccgggt
      601 aattaaagta ttgtgagatg ttcgtcgcga gatggtagga tgcagaaagg ctgcaagatt
      661 tcccggcttt gaattcagtc ttttcataag taccattagg attgcttagg aggttatgga
      721 tacgcaatga aaaaaaacat tatttggaga tttgtgctta tcgggattac cgttgtcctg
      781 gcgattgtct tttttcttcc caatatgccc cttttccagt atatgccgga gtggtggaag
      841 aataccatgc ccaacaaggg gataaccctc gggctcgatc tccagggagg cgtccatctt
      901 gtgttcgagg tggagggaga cagggcggtc gagatcacca cagagaggat agctcagtcc
      961 ttgaaggata tgctagggag aaaaaaactc actgcagagg tgagcaggaa cggccttttg
     1021 atcacggtga gccccaattc catggaggtg agaaaggccg tggaagagag ctatcccata
     1081 ctcaccccta cggagacagg gacaaaactt gtctacaagc tctcagaccg tgaggcagag
     1141 cggataaagg acaatgccgc aaaccaggca ttggaaacca ttcgtaacag gattgatcag
     1201 ttcggcgttg cagagcccac aatccaccgc caggcagcca atgaaatcgt ggtacagctg
     1261 cccggaataa aagacccgaa gcgcgccatt gagatcatag ggaaaactgc acagctcgag
     1321 tttaagatgg tggatgattc ttcacctgtt gcggctgaaa tccccgcatc ggtcagggca
     1381 ggggaagagg atgtgttgct cgcaaaattt tcaggtaaga taccagctga tgacgaaata
     1441 ctcttcgaga gggtcgtgaa cagagagacc ggcgaggtga caaaacgtcc cgtccttctg
     1501 aaaaagcaga cgatgatgac aggagacctc ctcaccgagg caaaggtgaa catagaccag
     1561 aggttcaacg agccttatgt ctcattaacc tttaatgccg caggtgcaaa gctttttgaa
     1621 gaaatcaccg gggccaatgt gaaaaagaga atggccatca tcctggatgg aaccgtgtat
     1681 tcggcacctg tgatccagga gaggataagc ggcggaaacg cacagatcac cgggaggttt
     1741 tccatggatg aggcgaggga tcttgccatc gtccttaggg caggcgcact tcccgcgccc
     1801 ataaagatgc tccagaacgt caccgttggt ccgtcgctcg gatcagactc catcgaggca
     1861 gggaaaaggg cgggaatcat agggaccatc cttgtagtag tcttcatgat cgtgtattac
     1921 cggctttctg gtgtgattgc agatttcgcc ctccttttga acatcatact ccttctcggc
     1981 gcaatggcgt cccttaacgc taccctcacc atgccgggta ttgcgggaat aatcctcgct
     2041 ataggcatgg cggtggactc caatgtgctg atgttcgaga ggatacggga cgaactcaag
     2101 gcaggcaaga ccccgagggc ctctgtggat tccggatatg acaaggcatt ctggaccata
     2161 ttcgattccc acgtgacgac actgatcact gcagcggtcc tcttccagtt cggtacagga
     2221 cccattaaag gatttgcggt aaccctaagt ctcggcgttg ccataaatct cttcaccgca
     2281 ctggtgggca caaaatccac atttgacctc ttgaacagca ggcaagaagt caaaaagctg
     2341 agcatatagg aggcaggatg ttagaactcg tcaaagacac aaagataaac ttcctcggaa
     2401 aaaggaagtt tgctttcata ttttcaggta tcctctccct ggtcgggatc ttcactattg
     2461 tccagatcgc caatggaaag gcaaatcttg gcatcgactt tgcggggggg accgcgatac
     2521 agctcaagtt cgagaaaccc ataaatctcc atgaagtgag aaaggccctg gaagacggtg
     2581 gcatcaggga ttttgacctc caggatctcc ccgccgtgca aaagatcctc atcagggaga
     2641 agaaaaccgt gcagcagctg ggacaggtat ccgacaccat caccggaata atctcgcaaa
     2701 aatttcctga taacaagtat gtggtggatt ccaccacgct cattgggccc aaggtcggag
     2761 ggaaattgag acaggatgct gcccaggcaa ttgtcctggc aacgctgggg atacttatct
     2821 atatcgcaat acggttcaaa ttcgattttg ccgtgggtgc aactgtcgcc acatttcacg
     2881 acgtacttgc agtgcttgcg atattctttc tcctcgggag ggagataaac cttatccttg
     2941 tcaccgcgct tctcaccatt gcgggatatt ccctcaccga cacggtggtg gtgttcgaca
     3001 ggataaggga gaacctccgc acaaggctca aggaaccggt agagactgtg atgaattcaa
     3061 gcatcaacga ggtgctttcc aggacaatca ttacctccct taccgtgctc atcacctcct
     3121 ttgccctctt cctttttggt ggagaggtga tccatgattt ctcacttgcc atgatccttg
     3181 gggttattat cggcacctac tcttccatct ttgtcgcaag ccctatagta ctgctctgga
     3241 gaaagaaaag gccatttgcg aaaaagtagt caacagcggt cgtccacagt cttcgttcac
     3301 actcctctaa ggggaagagc ggagacgagt gacccttggt ctcttcccgt ccttatatga
     3361 acaggcgatg gttcataagc agggcaaacc ccgaatatgt tcgttacctt tcaaaggctg
     3421 catccatatc ccctattctc acacaggtgt tgctaaagcg gggattcaag acccctggtg
     3481 acattaccga tttcctcact cccggacttg cccagctgac tgatccttat gaactccccc
     3541 atatgaaggc tgcggtggag aggatcaagg gagcgctcaa aagcaacgag agggtgctcg
     3601 ttcacggaga ttatgatact gacggattga cggccgcatc aattatggtc catgccctga
     3661 gagtgatggg ccttgatgcc cgttacttta tccccaacag gatgattcat ggatatggct
     3721 ttaaccagcc cggcgtggat gaagcaaaga aaacaggggt gaagctcatc atcaccgtgg
     3781 actgcggcat cacttccttt gcagctgcgg cgtacgcaaa aaaagaagga attgatgtca
     3841 tcatcactga ccaccatgaa ccgaaccgaa gggttcagga gagcaactca cgctcaatgg
     3901 tctctggtca acagccggaa gcaaaggacg aattcctgct gcccgatgcg acagtcattg
     3961 ttaaccctaa actgggaaaa aatcccggtc tctccgtcct ctccggcgcc gggatcgcat
     4021 ttaaaatggt ccaggccttt gcaatggatg atgccttacc cgtcaccgcg gatgattctc
     4081 tctcccttct tgacctcgct gccctcggaa cattagcaga cgtggtccca ttgactggag
     4141 agaacaggat catcctgaaa gaaggattac gaagcctgca acactcccac aggcccggga
     4201 taagatccct tttggaagtg tccggcctta aggaaagaga agtgaggagc ggacttttgt
     4261 cctttaccct ggtacccaga atcaatgcat ccggcagaat gggcgatgcc agcgatgtca
     4321 tgagactgtt tctgtctgat tcggaagggg agacgctttc catcgcggaa agactggaca
     4381 tgatcaatgc cgaaaggcag agaattgaag aagaggtgta tcaggaagcc ctttcccaac
     4441 tgaacagaaa aggatacgat gccgcaattg tgctttacga caaaggctgg cacgcaggtg
     4501 ttacggggat tgtggcgtcc agggttgctg aggaattctt caggccaacc tttgtgtttt
     4561 ccgtggaaga caccaccgcg aagggttctg tgaggagcat cccccccttc gacgtctatc
     4621 ggggactctc cgcatgcagc gacctcctcc tctccttcgg agggcataga caggcagctg
     4681 gggttaagct caatgtctca cacatgcctc tatttgaaga aaggatgaac actatcgtga
     4741 gggcttccct ttctgacgaa gatttcattc gcacggtaga gattgatgca gaggtcttcc
     4801 ttttggaagt cacgaacaat cttatcaagg agttttccgt gcttgaaccc ctgggtttcg
     4861 ggaaccctga accactcctg ggcgcaaagg aacttgcctt cctgaaccca agggttgtgg
     4921 gaaagaacca tctcaagatg accctgagac aaactcaggg gaaacaaagg tccctttcca
     4981 tggatgccat agggttttcc atggggaaat acctgcttca tctcgacacc gccacaaccc
     5041 tggacgcggc gtttaccccc atgataaatg agtggaacgg caacaaatac ctccagttac
     5101 acctcaaggc gcttagaccg agtccctcat tgtaggggta cctccttcat ttttctttgg
     5161 ggttcctgag tgttataatg aaaaaacgat ttgaacccat tggaggaatt aggcatgaag
     5221 aaatggacta tagatcctga tcacactgtg gccacattta gcgttcggca catgatggtt
     5281 gcaaatgtgc atggccagtt taacaagatt tccggtacaa ttcactttga tcctgctgat
     5341 atcccgcatt catccgtgga ggtgagcatt gatgccgccg ccatctacac aggcattccc
     5401 aaacgtgacg aacacctccg cagtgaggat ttccttgacg caggaaatca tcccagcata
     5461 accttcaaga gcacgaaggc cgaaggcaca gggagcaacc gtttcagggt caccggggac
     5521 ctcacgatac gggggattac gcgccagatc acctttgaag cagagcattt tggaccggtg
     5581 aaagacccct tcgaagagag tggaaggagc atcggctttg tcgcttccgc aacgatcaac
     5641 cgtgaagagt acgggatgac atggaacgtt gccatggaaa gcggcggcgt tatggtcggg
     5701 aaagaggtca cgattaccct ggaggtggag gccgatctct tcacttaata agaggctacc
     5761 gtagctaccg tagtgaggct gacttatctt ctcaaacccg tcctgacgtt aaactttagt
     5821 ccgggtgacg ttcttcgtca ccttcttgcc cgggtaacgt tcttgtgagt cattacgata
     5881 tcctttcttg tatttaccca tctcagtttt tatggtattc ttctttatga caaaaaacaa
     5941 tttcttgagg gagcacagag catggccttt accggggatt tagaactact taacatagtg
     6001 gacatcattc aactcctcaa tacgacacgg aaatccggca cgctctctgt aaggggactt
     6061 aagggcgaaa gccggattat tttcagtaat ggctacattg tgggagcaag ccatctcaac
     6121 aaccgggttc gcatcggaac agtcctggta aaaatgaacg ccatatcccc cgaatatctg
     6181 cagcaggcgc tggaggttca gaagcaggcg gggaaagacc gtaagccttt gattgccacg
     6241 cttctggaac tgggcaaact gaagagggac gaggcggcca tgggactgaa gaaactcatc
     6301 gagatgaccc ttgttgaagt gataggctgg acagagggga ccttcaccct cgacacagag
     6361 gccatcgccg tgtctcccga gagctcttat cccatcagca ggatggagca ggaaataggc
     6421 ctcgatgcac aagtggtatt gatggacgcc ctccgtattc ttgacgaacg tgaacgtgac
     6481 cgtcagtccg ggaaggctgt cccatcggat gaagaagtct tcgcagatgt gatttcatca
     6541 gaaggaccag gggcagccgg ggaggagagt tcgataaccg cagatgacct tggcttgggc
     6601 gatctcgatc atcttgagag gaaaatgccg cagtatatcc aggtggatga agtctttgac
     6661 cccattgagg tccaccgcca gaagataaga cagaccctcg cggcatttcc tgccgaaggc
     6721 caggaggcct ttgtcacttt tctggaaaaa tcttcgcgaa acagaggagc ctctgacaaa
     6781 tcccagagac aggcaggcag gacaagggcc cttgtccttt tcagcgagga tgaactcatc
     6841 aaacattcgg tgatgaccct atgcaaggat gagggcatcc ttgtatttgc cacggacgga
     6901 gaagaggaac tcgatcgaat tgttgaccag tgccttagga taaaagtctt ccctgtcctg
     6961 gccttcgata ccccgggaac aacggagcga ttactttcca aagagaagat cgtcagcctg
     7021 cgccagcagc tgaaggaacg atatccacag gttccgatta ttcagatggc ctctcctccg
     7081 gaccgccttt ttactttaca gtcttatcgg gacggcatca gggcagtctt tcccaagccc
     7141 tcacaggacg tcagtaagga cacgttcatt gcagatacaa tcacttttct ggaaaccttc
     7201 agatcatatg tcaggcgtct cttccttgag cagaaagacc tgaccgcaga ggacagcaag
     7261 cttgtgacgc ttaaagaccg cattgtggcc cttcgaagcc ttgacgaccc ttccgcggta
     7321 tccgtggccc tccttgagag cgtctctgaa gtgtacgaac gcgctgttat ctttattgtt
     7381 cgccagcaag agctcgtggg agacaaagcc atcggcgtat acgccgagcg ggaagaggga
     7441 gcaacgtccg cgacaggatt aaggatccct ctcaccgcac cttcgctctt ccgcaatgct
     7501 gttgagaagg ggcagttttt ctgtggcgag agcgacgatg atgtcctgaa gaaaagtctc
     7561 tttgaagaga tcgggacgcc tctgagggcg tcgataatcc tcatcccgat gaggagccac
     7621 ggaaagatcg tgacccttac atatggggat tttggcgaca aggaggcggc tccggtgcag
     7681 ggcgaagtgc tggagattct tgcacaatcg gccggccaga tggtggagaa tattctttat
     7741 cgcaaactgc tcagcaaggc gtcccaaaag tgagaaaatt caacacttat taggtaacca
     7801 taacaccatt caagggggta atgaccatgg atattgccac gttcacacaa attctcgaaa
     7861 tcgcttttca aaagaaggtc tccgacatcc actttgaagt ggataatccc cccatattca
     7921 gggcccgcgg gcacctgatc tactcaaaac tgagcaagct gacctggcag gacacccagt
     7981 ttatcgcttc cactgtcatg gaacagcacg gcaggaaact gccggaacgt ctcacggaat
     8041 tcgacacttc ttatacattg tcaaacgtgg gccgtttccg ggtcagcgtc ttccgtcagc
     8101 ggggcaacat cggccttgtg atgcgggtca tcccccacta catcggcacg tttgaggaac
     8161 tgaatctccc caccgtactg gggaagatca ctgaggcccc aaacggcctt attcttgtgt
     8221 gcggtcccac cggcaacggc aaatccacaa cccttgcctc gatgatccgg tatatcaatg
     8281 atcatttcaa ttacaacatt gttaccatcg aggaccctat cgagtttatt tttcattcaa
     8341 acaaaagctg tatcatccag agagaagtgg gtatcgatac agaaagtttt gacaaggcct
     8401 taaaagcggc catgcgcatg gatcccgacg ttatcatggt cggtgagatg cgcgatttgg
     8461 aaacaattga cgcgtgcatc aaggctgcag agacagggca tctggtcctt tcgactctcc
     8521 atacgcatag tgcggcctct accatcaacc gtgtggtggg gtatttcccg acgcaggtgc
     8581 aggagaacat ccgccacaga ctggcggaga gtctcgtggc caccatttct ttgcggcttg
     8641 taaggggcaa gggcggcgag tccattttgc ctgccctgga ggtgatgtat gcgacatcca
     8701 ccattaaggc atgtatccgc gacgggcatc tcgatgagat tgaacagtat atagagaagg
     8761 gacgggatga atatcagatg cagacccttg atcagcatct gatccaactg gtcaggcagg
     8821 aagccattgc tcttgatgag gcaaagcgga tttcccgctc tacggacctg gagacaaagc
     8881 tgatctatta ttagatagca ctgagaatag gcattttcac aaaagatgga aaagccctct
     8941 ccagtctccc gggctaaaaa ggcgattcac aacaaccctg acattattaa tagccattta
     9001 aattattcag gtacacaata caaatattaa ttgtagctat aggctatgga aaggataacg
     9061 atggaaccct cggcgagacg acatatcgga ggtcaaggca aaaaaattga agtcgacgca
     9121 gacggtaatc taaaatctgc cgaggattgg tgcgaggaat tagcaattta cttgagcgaa
     9181 gaagatggga ttaaactttc aaaggaccac tgggaggtta tcaatttcgt gcgctggtat
     9241 tacttaacct accagaactg tcctcatccc aaatttattg tcaaaaacct caacaagaag
     9301 tttggggacg gaaaatacac cgtcaaatct ctctacgatt tgttcaaaaa gcaaccagta
     9361 atgaaagcat gcaagcatgc cggcattcct ttcaccgctg gttgtacctg atacatacaa
     9421 tagttaggct ggacgtctaa ccacagcgtt tatcgctaag gtgccaccgc tgaactacgg
     9481 tgttatgcca cattaccctc ggaaatccgc attagttgca acttttttaa agtgaagact
     9541 tcggtgttat caacactcgc aggcatcctt tttcgctaaa tcaatttccc ttaggc
//
LOCUS       DATKOU010000082         5691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_101355, whole genome shotgun sequence.
ACCESSION   DATKOU010000082 DATKOU010000000
VERSION     DATKOU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101355"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..829
                     /locus_tag="VMU21_03860"
     CDS             23..829
                     /locus_tag="VMU21_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HUO76692.1"
                     /translation="MAIVTITRQAGSGGEDIGKGSAERLGYVYVGKERTFQELEKHGE
                     RWLDWGRNLDEHCPTLWEHYDPSFSGFVSLIEENILNYAVKDKAVIMGRGAHWLLRDI
                     PHVLRILIIAPLEVRINRIIDHEHVNGETATRLVEFSDHERSCYIHAVYRRNWVDPGD
                     YDVMFDTGRLSYEEVIKMVLDEIPERDKQATHEAKERLQRLALAAKVKAEIATDFRFF
                     VPTLEVVHDGSMIVVRGIVHSIKEHKMVENIARAVAAPTEIKCELHYRAK"
     gene            936..2000
                     /locus_tag="VMU21_03865"
     CDS             936..2000
                     /locus_tag="VMU21_03865"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HUO76693.1"
                     /translation="MSKTYKIAVIAGDGTGPEVVAEGTKVLNAAARKYGFKLDYTSFD
                     FGGERYLKTGELLPETIFDDLRKFDAIYLGAIGHPEVKPGVLEKGILLRMRFELDQYI
                     NLRPVKLFPGVDCPLKNKKPEDIDFVVVRENTEGLYTGAGGFLRKGTPDEIATQVSVN
                     TRKGAERCIRFAFDLTKKRNKAHKLTLCGKTNVLTYAFDLWERAFYEVGKEYPGIKTD
                     YAHVDAITMWFVKNPEWFDVIVTDNLFGDIITDLGAMIQGGLGIAAGGNINPKGVSMF
                     EPMGGSAPKYKGKNVINPLAAILAAGLMLDNLGEAKGAEAVEKAVMELTATKIKGLAA
                     GSMGYSTTEVGDMAADIVGR"
     gene            2134..4791
                     /gene="ppdK"
                     /locus_tag="VMU21_03870"
     CDS             2134..4791
                     /gene="ppdK"
                     /locus_tag="VMU21_03870"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HUO76694.1"
                     /translation="MAVKKHVYFFGSGKADGSGKMKDLLGGKGAGLAEMTNLKIPVPA
                     GFTITTEACNEYFRNKKKYPPGMWDQVLANLKKVEKAMGMKLGDKENPLLVSVRSGAK
                     FSMPGMMDTVLNLGLNGTTLQALIRKTGNERFAYDAYRRFITMFGTIVMGIERQVFEK
                     ALDRMKEKKGVHLDTQLPAEDLKELVREFKGIYQAETGTEFPEDALEQLKLAINAVFG
                     SWFGDRAVKYRKLNAIPDNLGTACNVQAMVFGNMGENSGTGVGFTRDPSTGEKRFFAE
                     CLINAQGEDVVAGIRTPLHIDELKKRLPAAYKELDSIYKKLEKHYTDMLDIEFTVQEG
                     KLYMLQTRVGKRTAAAALRIAIDMVKEKLIDKKTAVMRIDPDQLDQLLHPMIDTKAAV
                     KVIAKGLPASPGAAVGKVVFSADDAEKAAARNEKVILVRAETSPEDIGGMYAAQGILT
                     ARGGMTSHAAVVARGMGKCCVAGCGDININEELKHFMVGPHVIKEGDYITLNGSIGQV
                     ILGQVPLIQPELTKDFGTIMKWSDEFRKIGVRANADTLVDARLSRQFGAEGIGLVRTE
                     HMFFEVDRIKAVREMILSDDTAGRRKALEKILPMQRGDFVAIFTEMKGLPVTIRLLDP
                     PLHEFLPKTDEELHLLAEDMNVSFEKLKARNKSLHEFNPMLGHRGCRLGITYPEIYEM
                     QVQAIIEAACEVAKKKIKVIPEIMIPLVADVREQEVMREMTVRIADEIIKKHKIKIPY
                     AVGCMIEIARAALTADEIATQAEFFSMGTNDLTQTTFGLSRDDAGRFLPYYIEKGILP
                     DDPFMTIDRKGVGAMMRMAVEKGRRTRKNLKIGICGEQVDPRSVEFCDEIGLTYVSAS
                     PYRIPIARLSGAHAALKRKMKKDLSKASV"
     gene            complement(4874..>5691)
                     /locus_tag="VMU21_03875"
     CDS             complement(4874..>5691)
                     /locus_tag="VMU21_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO76695.1"
                     /translation="IKDLSTSEEALQESEEKFRSLVETTDNSIYVVNRNGEYLFMNAK
                     HRTRLGMVFDKEYMGKAFSDFHTPEEVVVFREKLNRVFETGEPLQQELRSARDGRYFL
                     RTLSPIKGPAKEVRAVSIVSKDITKLKKEGEAERRYHAIFEQSPYGILIMDTNGNIVE
                     FNETARRELGYSREEFAKLRLFDINPQSTEEIHDRIREVLNKGSCEFEVKHKTKNGEI
                     RDVRVNARALKLFGREVIQAIWQDITDRKRVEEALQQYREQLEKLVIERSRDS"
BASE COUNT         1492 a         1330 c         1584 g         1285 t
ORIGIN      
        1 cgactgagga aggagagcga taatggccat tgtgacaata accaggcagg cggggagtgg
       61 cggtgaggat attggaaaag gtagcgccga gaggcttggc tatgtgtatg tgggaaaaga
      121 acggacattc caggagctcg aaaaacacgg ggaaaggtgg ctggattggg ggaggaatct
      181 ggacgagcac tgccccaccc tctgggagca ttatgacccc tctttttccg gatttgtgtc
      241 cctgatcgaa gagaacatcc ttaattatgc ggtgaaggac aaggcagtca tcatggggag
      301 gggtgcccac tggctcctca gggatatccc ccacgttcta agaatcctga ttatcgcccc
      361 gctggaggtg agaataaatc ggataatcga ccacgaacat gtcaatggag agacagcaac
      421 ccgtctcgtg gagttcagcg atcatgagag gtcatgttac atacatgcgg tgtaccgcag
      481 gaactgggtt gatcccggcg actatgacgt gatgttcgat accggccgcc tcagttacga
      541 agaggtgata aagatggtgc tggatgaaat ccctgaacgg gacaaacagg cgacccatga
      601 agcaaaggag agattacaac gtcttgccct ggcagcgaag gtcaaggcag agatcgcaac
      661 agacttcagg ttctttgtcc ccacactgga ggttgttcac gacggctcaa tgattgtggt
      721 acgcggcatt gtacacagca tcaaggaaca caaaatggtg gagaatatag ccagggctgt
      781 agctgcaccc acagagataa aatgtgaact tcactaccgt gcaaaatagg aagaagggcg
      841 aattgtctgc cacttttgga gactatttgt tataatattt gcccctctgc aggggtgagc
      901 tttttggaga atatccgtca aagaggagga gatgtatgtc gaaaacctat aaaatagcag
      961 ttattgctgg tgacgggacc ggcccggagg ttgtggcaga agggaccaag gtgctgaatg
     1021 cagcagcccg gaaatacggg ttcaaactgg attacacatc gtttgatttc ggcggcgaac
     1081 gatatctgaa gaccggcgaa ctccttcccg agaccatatt tgatgacctg agaaagtttg
     1141 acgccatcta cctgggcgcg atcgggcacc ctgaggtaaa accgggggtc ctggaaaagg
     1201 gcatactcct caggatgagg tttgagctgg accaatatat caatctccgt cccgtgaagc
     1261 tctttcccgg ggttgactgc ccgctgaaaa acaaaaaacc cgaggacatc gactttgtgg
     1321 tggtgaggga aaacacggaa gggctctata ccggtgcagg cggattcctg cggaagggaa
     1381 cccctgatga gatcgcaacc caggtctcgg tgaatacaag gaagggtgcg gaacgatgta
     1441 tccggttcgc ctttgacctt accaaaaaga ggaacaaggc gcacaagctc accctctgcg
     1501 gcaagacaaa tgtccttacc tatgccttcg atctttggga aagggcattc tatgaagtgg
     1561 gaaaggagta tcctggcata aagaccgact atgcccatgt ggatgccatt accatgtggt
     1621 ttgtgaaaaa ccctgaatgg ttcgatgtga ttgtcacgga caatctgttt ggggacatta
     1681 ttaccgacct tggagccatg atccagggag ggctcggcat tgctgcggga gggaatatca
     1741 atcccaaagg ggtctcgatg ttcgagccca tgggtggttc tgccccgaaa tataagggga
     1801 aaaacgtcat aaaccccctt gcggcaatcc tggcagccgg cctgatgctc gataatctcg
     1861 gggaagccaa gggagcagaa gcggtggaaa aggcagtaat ggaactcacc gcaaccaaga
     1921 tcaagggcct cgctgcaggg agcatggggt attccaccac agaagtgggg gatatggctg
     1981 cggatattgt cgggagatag atttttcttc ccaaaaaacg gcggttatgg taaaattttt
     2041 tgctttcaag acggggagag tcttgagaaa aaacaacatc ctaaataatt caaaaaaagt
     2101 gatggttcgc aaattcttcg caggaggcat gctatggctg taaaaaaaca tgtgtacttc
     2161 tttggcagtg gaaaggccga tgggagcggc aagatgaagg acctgttggg cggtaaggga
     2221 gcgggacttg ccgagatgac aaacctcaag atcccggttc ccgcaggatt taccattacc
     2281 accgaggctt gcaacgaata cttcagaaac aaaaagaaat atccgcccgg catgtgggac
     2341 caggtgctgg cgaacctcaa gaaggtagag aaggcgatgg gaatgaagct tggggacaaa
     2401 gagaatcccc tccttgtttc ggttcgttca ggggccaagt tttccatgcc cggcatgatg
     2461 gatacagtcc tgaacctcgg tcttaacgga accactctcc aggcccttat caggaaaacc
     2521 ggaaatgagc gctttgccta tgacgcatac agaaggttca ttaccatgtt cggaacaatc
     2581 gttatgggga tagagaggca ggtattcgaa aaggcccttg acagaatgaa ggagaagaaa
     2641 ggcgttcatc tcgacacaca gcttcccgca gaggacctca aggaactggt tagggaattc
     2701 aaggggattt atcaggcaga gacagggacc gaatttcccg aagacgccct tgagcagctt
     2761 aaacttgcca tcaacgcggt cttcgggtca tggttcgggg acagggcggt caagtacagg
     2821 aagctcaacg ccataccgga caacctcgga actgcgtgta atgtccaggc catggtcttt
     2881 ggaaatatgg gagaaaattc gggaaccggt gtaggcttca caagagatcc ctctaccggg
     2941 gagaaacggt tctttgcgga atgcctcatc aatgcccagg gtgaagacgt agttgcgggt
     3001 ataaggactc ctcttcacat agatgaactg aagaagaggt tgcccgccgc ctataaggag
     3061 cttgacagca tttacaagaa gctcgagaag cattatacgg acatgcttga tatcgagttc
     3121 acggttcagg aaggcaagct ttacatgctc cagaccaggg ttggtaagag gactgctgcg
     3181 gcggcactca ggattgccat cgacatggta aaggagaagc ttatcgacaa gaagaccgcg
     3241 gtcatgagga tagaccctga ccagctggac cagcttctcc accccatgat agataccaag
     3301 gcagctgtca aggtcattgc caagggactt cccgcctcgc ccggagctgc ggtgggtaag
     3361 gttgtcttct ccgcggatga tgccgagaaa gcggcagcac gcaatgagaa ggtcatcttg
     3421 gtgagagccg agacatcccc tgaagatata gggggaatgt atgcggccca gggcatactc
     3481 actgcccggg gagggatgac ctcccatgca gcggttgtgg caaggggcat ggggaaatgc
     3541 tgcgtggctg gctgcggtga catcaatatc aatgaagagc tgaaacattt tatggtgggt
     3601 ccccatgtca tcaaagaggg ggattacatc accctcaatg gaagcatcgg tcaggtaatc
     3661 ctcggacagg ttcctctcat acagcccgaa cttaccaaag acttcggcac gatcatgaag
     3721 tggagcgatg aattcaggaa aataggggtg agggcaaatg ccgatacact tgttgatgcc
     3781 cgtctgtcga ggcagttcgg tgcagaaggc ataggactcg tgaggacaga gcatatgttc
     3841 tttgaggttg acaggatcaa ggcggtgcgt gagatgatcc tctcagacga taccgcgggc
     3901 aggagaaagg cccttgagaa gattttaccc atgcagaggg gcgattttgt cgccatattc
     3961 accgagatga agggtctgcc ggtgaccata cggctccttg accctccgtt gcatgagttt
     4021 ctccccaaga cggacgagga actccatctg cttgcggaag acatgaatgt ctccttcgag
     4081 aagcttaagg cgaggaataa gtccctccat gagtttaacc ccatgctcgg ccacagggga
     4141 tgcaggcttg gcatcaccta ccctgagatc tacgagatgc aggtccaggc gatcattgag
     4201 gccgcctgtg aggttgcgaa gaaaaagata aaggtcattc ccgagatcat gatccccctg
     4261 gttgcagatg tgagggaaca ggaggttatg agggagatga cggtgcgcat tgcagatgag
     4321 attatcaaga agcataagat aaagattcct tacgccgtag ggtgcatgat cgaaattgcc
     4381 cgcgctgccc tgaccgcgga tgagatagca acgcaggcgg agttcttctc catgggcacg
     4441 aatgacctta cccagaccac cttcgggctc tcacgtgacg acgcaggcag gttcctcccc
     4501 tactatatag aaaaggggat actgccggat gatcccttca tgaccattga tcgcaaaggg
     4561 gttggtgcga tgatgaggat ggcggttgaa aagggtagga gaaccaggaa gaatctgaag
     4621 atagggattt gcggggaaca ggtggatccc aggtcagtag agttctgcga tgagatcgga
     4681 cttacctatg tgagcgcttc accttaccgg attcccattg cccgtctctc gggagcacat
     4741 gcagcgctca agaggaaaat gaaaaaggac ctgagcaagg catcggtctg acgcggtcac
     4801 cctgaattgt caaaaggctt ctcttacatg cacgtaagag aagcattttc attgggtgga
     4861 ctatttacac gccctagctg tcccggctgc gctctataac caatttttcc aattgttcac
     4921 ggtactgttg taatgcttcc tcgacccgtt ttcggtctgt gatgtcctgc catatggctt
     4981 gaatgacttc gcgaccgaat aacttcagtg cccgtgcatt cacacggaca tcccttattt
     5041 ccccgttttt ggttttatgc ttcacttcga attcgcagct gcctttattt aacacttccc
     5101 tgatcctgtc gtgtatctct tctgtactct ggggattgat atcaaagaga cggagcttcg
     5161 cgaactcctc cctcgaatac ccaagctcac ggcgagccgt ctcattgaat tcaactatat
     5221 tcccgttcgt atccatgatt aaaataccat aaggagactg ttcaaatatt gcatggtaac
     5281 ggcgttctgc ttcaccctct tttttcagtt tggtgatgtc tttagaaacg atactcactg
     5341 ctctcacctc tttggccggt cctttgatcg ggctcaatgt gcggagaaag tatctgccgt
     5401 ccctcgcact tctcagttct tgctgcaacg gttcgcctgt ctcaaatacc ctgttcaatt
     5461 tctcgcggaa aacgactact tcttccggcg tatgaaagtc gctaaaggcc tttcccatat
     5521 actctttatc gaagaccata cccagtctgg tacggtgctt ggcgttcata aacaaatatt
     5581 cgccattcct gttcaccaca tatattgaat tatcggtggt ctcaacgaga gagcgaaact
     5641 tttcctcact ctcctgaagt gcctcttcag acgtactgag gtctttgatg t
//
LOCUS       DATKOU010000083         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_873052, whole genome shotgun sequence.
ACCESSION   DATKOU010000083 DATKOU010000000
VERSION     DATKOU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873052"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VMU21_03880"
     CDS             complement(<1..235)
                     /locus_tag="VMU21_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76696.1"
                     /translation="MRILLRPFEAFGEVKVFLISLSLIIALFLSLIFLGGYLRSNTLL
                     FETVRDEARSYFALIVQTRLWNARYGGVYVEKQG"
     gene            400..2133
                     /locus_tag="VMU21_03885"
     CDS             400..2133
                     /locus_tag="VMU21_03885"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HUO76697.1"
                     /translation="MKTVRIALAQINPIVGDLEGNTEKIIHYIRRAEENSSDIVAFPE
                     LSVSGYPPEDLLLKPQFIKDNLDALRRIKEEVWDITAIVGFVDRKNDIYNAAALLHNK
                     TLVDTYHKMYLPNYGVFDEFRYFQAGTRFPLYGIGDVTVGVNICEDIWYPEGPAHIQS
                     LFGAEVIININASPYHIGKAAFREKMLSTRATDNAVAIAYLNTVGGQDELVFDGHSLV
                     IDEKGEIICRGKQFEEDLIIVDLNLDAVFIRRLHDPRRRQEEIAAAERSKVEKIVISH
                     ERPQRTAKPVPRRESRIMDPVEEVYNALVLATADYVTKNRFWSVIIGMSGGVDSSLVA
                     TIAVDALGKNNVKGLFMPSKYTSMESREDAAGLAQNLDIELMEVPITGIYSAYLETLE
                     KEFKGLPPDATEENLQARIRGNILMAFSNKFGWLVLTTGNKSEMSVGYATLYGDMAGG
                     FAVIKDVPKTMVYDLCRWRNTKGDKALMPERVLWKEPSAELKPDQKDTDVLPPYQVLD
                     PVLKAYVEDDRSFEELASLGCEAECVEKIISMIDKSEYKRRQSPPGIKITQRAFGRDR
                     RFPITSRYKSY"
     gene            2228..2479
                     /locus_tag="VMU21_03890"
     CDS             2228..2479
                     /locus_tag="VMU21_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76698.1"
                     /translation="MGFLKGLIIVLLTLWCTGIVVAAEPSDIQFTKQPEIQQVRPQKP
                     VKIRLHRNAKGEYSWDLNGDNVDEIVNADKKLRKLLKVQ"
BASE COUNT          762 a          562 c          719 g          634 t
ORIGIN      
        1 ctccttgttt ctccacatac accccgccgt atctcgcatt ccatagcctg gtctggacaa
       61 tcagtgcaaa gtatgacctg gcctcatccc tcacggtctc gaatagaagg gtgttactcc
      121 tcagataccc cccgaggaaa atgagagaaa gaaagagcgc gatgatgagg ctcagggaga
      181 tgagaaacac ctttacttca ccaaaagcct caaaaggtct tagcaatatt ctcatcagtg
      241 gatgggcctt cctgcaaaga tctctgctcc cgataactga ttgtcatatc cttaccccat
      301 tataatacat atcgggggat ggaattgaag gtatttcgtt tatttcttgc gtagatgctt
      361 tacctttaca aaagggccct gtttcattat tatttaaaga tgaagaccgt gagaattgcc
      421 cttgcccaga ttaaccccat tgtaggggac ctggagggca atacggagaa gatcatccat
      481 tacatccgga gggcggaaga gaattcctct gatatcgtcg ctttccctga actttcggtg
      541 agcggctacc ctcccgaaga cctcctgcta aagccccagt tcataaagga caacctggat
      601 gcccttagga gaatcaagga agaggtctgg gatatcaccg caatcgtggg ctttgtggac
      661 aggaaaaatg atatctataa tgccgctgcc ctgcttcata acaagacctt ggtagatacc
      721 tatcacaaga tgtaccttcc caattacggt gtcttcgatg aattcaggta tttccaggca
      781 ggtaccaggt ttcctctcta tggtattggt gatgtcaccg ttggggtgaa catatgtgag
      841 gatatatggt atcccgaagg gcctgcgcac attcagtccc tgtttggggc agaggtaatc
      901 atcaatatca acgcatctcc ttaccatatc ggcaaggcag cgttcaggga gaagatgctc
      961 tccacaaggg caacggataa tgcggtagca attgcatatc tgaataccgt ggggggccag
     1021 gatgaacttg tctttgacgg acacagcctt gtgatcgatg aaaaaggcga gattatctgc
     1081 cgggggaaac agtttgaaga agacctcatc attgtggacc tgaatctcga tgcggtattt
     1141 atacggaggc tccatgaccc acgcagaaga caggaagaga tcgcagctgc ggaacggtcg
     1201 aaggtggaaa agatcgtgat ttcccatgag aggcctcaga gaacagcgaa gccagtaccc
     1261 aggagagaga gccggattat ggaccctgtt gaggaggtct ataatgccct tgtgctcgca
     1321 acggctgact atgtgaccaa aaacaggttc tggtctgtga tcatcggaat gagcggagga
     1381 gttgactcaa gccttgtggc aactattgcg gtggatgccc tagggaagaa taatgtgaaa
     1441 gggctgttca tgccatcaaa atatacctca atggaaagca gagaagatgc ggccggactt
     1501 gcccaaaacc tggatatcga acttatggaa gttcccatta cagggatcta tagcgcgtat
     1561 cttgagacac tggaaaaaga atttaaaggt ctcccccctg atgccactga agagaatctc
     1621 caggcgagga tcaggggaaa cattcttatg gcattctcga ataaatttgg ctggctggtg
     1681 ttgaccacag ggaacaagtc agagatgagt gtcggatatg cgacgctcta tggagatatg
     1741 gcagggggtt tcgcggtgat caaggatgtt ccaaaaacca tggtgtatga tttgtgcaga
     1801 tggaggaata cgaagggaga taaggcactg atgcctgaga gggtattgtg gaaagagccg
     1861 tctgctgagc ttaaacctga tcagaaggac acagatgtac tccctcccta tcaggtgctt
     1921 gaccctgtcc tgaaggccta tgtggaagac gacaggagtt tcgaggaact ggcctcgctt
     1981 gggtgtgagg ctgagtgtgt ggagaagatc atcagcatga tcgacaaaag cgagtataag
     2041 aggaggcaat ctcccccagg gataaaaatc acccaaaggg cttttggaag ggaccggagg
     2101 tttcccatta caagccgcta taaaagttat tgaaaaggca cacggagatg catcctggaa
     2161 tgtgcatggt tcttctcccg cttcatgcgg aggaatattc atgttttgta tttgtgagga
     2221 ggcagggatg ggctttttaa agggattaat aattgtcttg ttgactttat ggtgtactgg
     2281 catagtcgtt gcggctgagc catccgacat tcagtttacc aaacagccgg aaatacagca
     2341 ggtgagaccg caaaagccgg tgaagataag attgcacagg aatgcaaagg gtgaatattc
     2401 atgggatctc aacggcgata atgttgatga aatcgtgaac gcagacaaaa aactgagaaa
     2461 gttattaaag gttcagtaag agattcagtg aggttctttt aaaggaggga gtgcatggag
     2521 aaatttgcga tcagagaggt tattgaacag gcagtacaga ccgagaagct gggatttcag
     2581 ttctacacca caacagcgga gaaattcgac aaagaaaagg atctccagaa gctcttctca
     2641 acccttgcgg ataaggaacg ggtgcacgaa aagcggt
//
LOCUS       DATKOU010000084         9994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2030189, whole genome shotgun sequence.
ACCESSION   DATKOU010000084 DATKOU010000000
VERSION     DATKOU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030189"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VMU21_03895"
     CDS             complement(<1..1145)
                     /locus_tag="VMU21_03895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO76699.1"
                     /translation="MSEKILVRGVNWMGDAVMTLPTLKALRKAHPESKISLLVKPAVA
                     AIFEKNPSIDEIILYEERFQGAFGKFILSQRLRKKHFSRTILFQNAFDAALITFLAGI
                     PQRTGYSRDGRGFLLTKPIPFNDDDRKMHHIEYYLNLLRASGISAENTQPWIHLSLEE
                     RLAARNALSRLERPVLGINPGAAYGSAKRWLPDRFAEVADWFINDTGGSVIIFGGKSE
                     EGVSQEIEKMVEGRKSKPAWALRRQSGGKGEGTGLDDTTLTTRIASIQSEDVSNSLLN
                     LAGKTSLRELISAISECDVFLSNDSGPMHVAYAVGTPLVALFGSTDPGLTGPAGEGSV
                     VIRHPLSCSPCFERSCKDKDLRCMYAITSDEVFLAIKKMLPKKSAIFL"
     gene            complement(1309..2019)
                     /locus_tag="VMU21_03900"
     CDS             complement(1309..2019)
                     /locus_tag="VMU21_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HUO76700.1"
                     /translation="MSCRTNNSGHLASLRTSLIINLNCIILLLAVSFTTSCSSARYQT
                     RGDTGSGVATWYGPEFHGKNTASGERFNMYDLTCAHKELPFGSVLEVTNTANGRSVKC
                     TVNDRGPFVSGRDLDLSYAAAKEIGLIGPGQARVKITYLGRDTSYVKEVRYVSSGGPY
                     TVQVGSFKELDNALHLKASLELKYKDVYIIEAKIKKTTHYRVRVGKFQRREEAFGLAK
                     TLADEGYSPLVTHYEERA"
     gene            complement(2205..3542)
                     /gene="hslU"
                     /locus_tag="VMU21_03905"
     CDS             complement(2205..3542)
                     /gene="hslU"
                     /locus_tag="VMU21_03905"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HUO76701.1"
                     /translation="MDNLTPKRIVEELDRYIIGQHKAKKAVAIALRNRWRRQRLSEDI
                     KDEVLPKNIIMIGPTGVGKTEIARRLARLANAPFLKIEASKFTEVGYVGRDVESLIRD
                     LTEISLNMVKTEHIEKVQERAKALAEDRLLDYLLPQPRTVRGVDTEEEREKYRETRER
                     LRQQFHEGKLDSRYVDIEVKEKSMPFGIVSNVGLEDLEINLKEMLGNFLPEKQKRKKV
                     RVPEAMNLLIQEEGNKLIDMEKVTKEAIQRVEQGGIVFIDEIDKIASKGSSYGPDVSR
                     EGVQRDLLPIVEGSTVTTKHGPVRTDHILFIAAGAFHMTKPSDLIPELQGRFPIRVEL
                     EPLGKDEFVRILKEPYNALTKQYRALLATEDMDIEFTDDAIDEISNIAAAVNEKMENI
                     GARRLHTVLEKLLEEVSFEAPERKNGKLVIDRAYVKSQLSDIVKDEDLSRYIL"
     gene            complement(3535..4068)
                     /gene="hslV"
                     /locus_tag="VMU21_03910"
     CDS             complement(3535..4068)
                     /gene="hslV"
                     /locus_tag="VMU21_03910"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HUO76702.1"
                     /translation="MIKGTTILCVRKDGKVAIGGDGQVTFGNTVLKHNAKKIRRMFNN
                     KVIAGFSGATADAFTLFEKFEAKLESYRGNLTRAAVELAKDWRTDKVLRRLEALLIIS
                     DAEHTFIISGTGDVIEPETGIAAIGSGGPYAQAAANALYENTGLSAKEIVEKAMEIAA
                     SICIYTNKVITIEELNG"
     gene            complement(4061..4957)
                     /gene="xerA"
                     /locus_tag="VMU21_03915"
     CDS             complement(4061..4957)
                     /gene="xerA"
                     /locus_tag="VMU21_03915"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HUO76703.1"
                     /translation="MKEEIEKFLRYLATERGASAHTIRSYRKDLDVFSEEIHSDVESV
                     DFMDVRGFVAAQMGKGLKKSTVGRRLATVRSFFRFMHREGIIKTNPARLVNTPKMPKL
                     LPKFLTVDDVFSLIEKPEGIGFLPARDRAILELLYSSGLRVGELSGLNAEDMNIREGL
                     VKVRGKGKKERIVPVGRKALEALKTYMIERVILKKKDKALFLNRGGLRLTDRGARRIV
                     VKYARAILLDSRIGPHTLRHTFATHLLQGGADLRVIQELLGHSSLSTTQKYTHLDITH
                     LMDVYDKAHPLAGENGKEHTRD"
     gene            complement(4954..6258)
                     /gene="trmFO"
                     /locus_tag="VMU21_03920"
     CDS             complement(4954..6258)
                     /gene="trmFO"
                     /locus_tag="VMU21_03920"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HUO76704.1"
                     /translation="MAGLIVIGGGLAGCEAAWQAARRGVEVTLFEMRPARLTEAHKTG
                     LLGELVCSNSLRSADILSAPGLLKKELEMAESLIMKAAVASRIPAGSALAVDRGAFAE
                     FLTNALSDSPLITIIREEVSALQDLPSVLATGPLTSGPMAESLKKLLGHEYLHFYDAI
                     APIIDAESIGYGKVYFGSRYGKGGDDYINCPMTKDEYETFFGALSEADRVNIRVFENE
                     KVFEGCMPVEVMALRGKDTLRFGPMKPVGLPAPKTGREPYAVVQLRPENRERSAYNMV
                     GFQTRLKWPEQKRVFSMIPGLEKAEFLRYGSVHRNTFINAPESLERDLTLKACPAVYL
                     AGQITGVEGYIESTAMGLVAGINASRKIRGKESVETPVTTAHGSLIHHITLPTKNFQP
                     SNVHFGLFPAVNSGIKKKELKRRMIVERAISDWSAYLEQVGA"
     gene            6601..7902
                     /locus_tag="VMU21_03925"
     CDS             6601..7902
                     /locus_tag="VMU21_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HUO76705.1"
                     /translation="MLSKWVYPLSLLLSFTLFPSSLFSKETPPEVMVITLDGVINPVA
                     SEFISKSIKKATKRGVEALVVELDTPGGLDTSMRMIVKEIVGSEVPVVVFVAPSGARA
                     ASAGVFITIAAHIAAMAPGTNIGAAHPVGLGEKMDKTIAEKATNDAAAYIKSLAERKG
                     RNAAWAEKAVRESISATEVEALRDHVIDIVSKDLTTLLVDINGRKIQTALAEKVLRTK
                     DAKITREETGLRQKILNFISDPNVAYILMLLGFYGLFFELTNPGAIFPGVIGGICLIL
                     AFYAFQTLPVNYAGLLLIILAIILFILEVKIVSHGVLTIGGVIAMIIGSLMLFETPGP
                     FVRLSLYLILPAVMVTALFFSLTIGLALKAHKRKPVTGKEELVGMEGVARTDITPEGG
                     MASLHGELWAAYSEERIAKDNKVVVESVSGLKLKVHKSKGG"
     gene            7904..8689
                     /locus_tag="VMU21_03930"
     CDS             7904..8689
                     /locus_tag="VMU21_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HUO76706.1"
                     /translation="MGNMPIIGPSILSMVFVIFLFFYFLSSAIKILKEYERGVVFRLG
                     RIIPIKGPGLVIIWPIIDKLVRVSLRTVTFDVPSQDIITKDNITVKVNAVVYFRVIDP
                     IRAITAVEDFFYATSQIAQTTLRSILGQSQLDDLLTKRDELNAELQKVIDFQTEPWGI
                     KVATVEVKNVDLPVEMQRAIAKQAEAERERRAKVIHADGEFQASQKLADAAQIMATQP
                     MAMQLRYLGTLTEIASEKNSTIIFPLPIDLIKPLFDRLEKKEQ"
     gene            8855..9475
                     /locus_tag="VMU21_03935"
     CDS             8855..9475
                     /locus_tag="VMU21_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HUO76707.1"
                     /translation="MRAFVDSFGPLGFMAFILLQVTQVVAAPIPGEVTGLLGGFLYGP
                     VLGVVLSTIGLTIGSYVAFALARVFGRPFVDKFVDKRVMKRFDYLLHHKGAFLVFLLF
                     LIPGFPKDYLCYILGLGHLSTMEFLVIGGTGRLFGTILLTLGGSYIRHHQYMRFFVLA
                     GVAIIVVLLAMVYRDRIERLFRKWHILKYKKTKKKKAQQNQTMSAK"
BASE COUNT         2380 a         2659 c         2339 g         2616 t
ORIGIN      
        1 agaaatattg ccgatttttt cgggagcatc ttttttatgg cgagaaagac ttcatccgaa
       61 gtgatggcat acatgcacct cagatctttg tccttacatg agcgctcaaa acaggggctg
      121 caggagagcg ggtgacgtat caccacactg ccctcccccg cagggcctgt gagacccgga
      181 tctgtggagc caaagagggc caccaagggg gtccccaccg cgtacgcgac atgcataggg
      241 cctgaatcat ttgaaaggaa cacatcgcat tccgaaatag cagaaataag ttctctgagg
      301 gacgttttcc ccgccagatt cagcaacgag ttactcacat cctctgactg tatcgaagcg
      361 atacgtgtgg taagtgtcgt atcatctaaa cccgtgccct cccccttgcc gccgctttgc
      421 ctccgcaaag cccacgcagg ttttgattta cgaccttcca ccattttttc aatctcctga
      481 gaaacgcctt cctcgctctt tcctccaaag attatgacac tccctccggt atcatttatg
      541 aaccaatcgg caacctctgc aaacctgtca ggcagccatc tctttgcaga gccataggca
      601 gcaccagggt ttatcccaag gaccggcctc tccagcctag agagggcatt gcgagctgcc
      661 aaacgttctt ccagagagag atgaatccaa ggttgcgtgt tctctgcgct tatccccgat
      721 gcccgtaaaa gattgagata gtactctata tgatgcatct tcctgtcatc gtcattgaag
      781 gggatgggtt ttgtgaggag aaatcccctc ccgtccctgc tataccccgt cctttgggga
      841 attcccgcga ggaaggttat caacgctgca tcaaatgcat tctggaagag gatggttctt
      901 gaaaaatgct tcttccttaa ccgttgggag agtatgaatt tcccgaaagc cccctggaac
      961 ctctcttcat aaaggatgat ctcatcaata gaggggttct tctcaaaaat cgctgcaaca
     1021 gcgggtttta ccaacaggct tatctttgat tcagggtggg cctttcttaa tgccttcagc
     1081 gtgggcaggg tcattaccgc atcacccatc cagttcacac ccctcacaag tatcttttct
     1141 gacatgtgtt aatttaccac aaaaagaaag gagggtgcgt taagagactg tgtcaatctt
     1201 cagctaatac gaaatggtgt gctcgttttc cccactcacc gacttttcaa aatgaacaat
     1261 gcattaaaaa agagaagcac tatataattt cctgtgacaa aagcagattc acgcacgttc
     1321 ctcgtaatgg gtgaccagag gactgtagcc ttcatctgcc aacgtctttg caagaccaaa
     1381 tgcttcctcc cttcgctgaa attttcccac cctcactcta taatgagtgg tcttcttaat
     1441 cttcgcttcg atgatataca catctttgta tttcagctcc aggcttgcct tcaggtgaag
     1501 ggcgttgtcc agttctttga atgagcctac ctgcaccgtg taaggcccgc ctgatgagac
     1561 ataccgcacc tccttcacat aactagtgtc ccttccgaga taggtgatct tcaccctcgc
     1621 ctgtccaggg cctatgaggc ctatctcctt tgcagctgcg taagagagat ccaggtccct
     1681 tcccgagaca aaaggtcccc ggtcattgac agtgcacttc actgacctgc cgttggcagt
     1741 attggtcact tccagcacag aaccaaaggg gagttcttta tgcgcgcagg tgaggtcata
     1801 catgttgaat ctctctcctg aggcagtgtt tttcccgtgg aattccgggc cgtaccatgt
     1861 tgccacaccg ctgcctgtat caccacgggt ctggtagcgg gctgaactgc aggatgtagt
     1921 aaaagaaact gcaagaagta ggattatgca gttgagattg atgatgaggc tcgtccggag
     1981 cgaggcaaga tgccctgagt tattggtgcg gcagctcatg cgccgcacag caccgaaatg
     2041 tgatgacatc gcgtttaggt gcacggacat aattcgtaag acaaagagcg aatgtgtctt
     2101 gccagaaccc ggatgggcct cataaacaca ccgtttattg acgatatacg gtaaggtctc
     2161 tgaaacacct agccaccgtg ctctctactc tttacccttg cccgttacag gatgtatctg
     2221 ctgaggtcct cgtccttcac tatatcggac agctgtgatt tcacataggc cctgtcgatg
     2281 accaatttcc cgttctttct ctcaggggcc tcaaatgata cctcctcaag gagtttttcc
     2341 agaaccgtgt ggagcctccg tgcccctatg ttctccattt tctcattcac tgctgcggca
     2401 atattcgaaa tctcatctat ggcatcatcg gtaaactcga tgtccatatc ctcggtggcc
     2461 agcaatgccc tgtactgctt ggtcagtgcg ttatagggct ccttgaggat gcgcacaaac
     2521 tcgtctttgc ccaggggctc cagttccacc cgtataggaa atcttccctg gagctcgggg
     2581 ataaggtcag agggctttgt catgtggaac gcacctgcag caatgaagag gatatggtct
     2641 gtgcgcactg gcccgtgctt tgtggtcact gtggagcctt ccacaatggg caggaggtcc
     2701 ctctgcacac cctcgcggga tacgtcaggg ccataggaag aacccttgct ggctatcttg
     2761 tcaatctcgt caataaatac tatcccaccc tgctccaccc gttgaatggc ctccttggtc
     2821 accttctcca tatcgatgag cttattgccc tcttcctgga taagaaggtt catggcttcc
     2881 gggaccctta cctttttcct cttctgtttt tcgggaagaa aattccccag catctccttg
     2941 agattaatct caaggtcttc aagccccaca ttggaaacaa tgccgaaggg catggacttt
     3001 tccttcactt caatgtcaac ataccttgaa tccagctttc cctcatggaa ctgctgtctc
     3061 agcctttccc tcgtctccct gtatttctcc ctctcttcct ctgtgtcaac ccctcgcacc
     3121 gtccttggct gggggaggag ataatcgagg agcctgtcct cagcaagggc tttggccctc
     3181 tcctggacct tttcgatatg ctctgtcttg accatattaa gggatatctc ggtgaggtcc
     3241 cttatgaggg actctacgtc gcggcccaca tagcccacct cggtgaactt tgatgcctca
     3301 atcttcagga aaggcgcatt ggccagtctg gcaagccgcc tcgcaatctc ggtcttcccc
     3361 acgcccgtgg gccctatcat gatgatgttc ttgggaagga cctcgtcctt gatgtcttcg
     3421 gagagccgct gcctcctcca tctgttcctc aatgcaatag ccaccgcctt tttggcctta
     3481 tgctggccaa tgatgtagcg gtcaagttcc tccactatcc tctttggggt aagattatcc
     3541 attcagctcc tctattgtta tgaccttatt ggtgtatata catatggacg cagcgatctc
     3601 cattgccttc tccacaatct ccttggcaga caagcctgta ttctcgtaaa gtgcgttcgc
     3661 agccgcctgc gcgtaaggac ctcctgaacc gattgcagca attcctgtct ccggctcgat
     3721 cacatctcct gtcccagaga tgatgaaggt atgttcagca tcagagatga tcaggagtgc
     3781 ctccagtctc cgcaatacct tgtcggtcct ccagtctttg gccagttcaa ccgcagccct
     3841 ggtgaggttg cccctgtatg actcgagctt tgcctcaaat ttttcgaaaa gggtaaaggc
     3901 atccgcagtg gctcctgaga agcctgcaat aaccttgtta ttgaacatcc tccttatctt
     3961 ttttgcattg tgtttgagca cggtattgcc gaaggtgacc tgaccatctc ccccgatggc
     4021 taccttcccg tcttttctga cacagagaat cgttgtccct ttaatcacgt gtatgctcct
     4081 tcccgttttc ccctgcaagg gggtgggcct tatcatagac atccatcagg tgcgtaatat
     4141 ccagatgggt atatttctgc gttgtggaaa gggatgaatg acccagaagc tcctgaatca
     4201 ccctcagatc agccccgccc tggagaaggt gcgttgcaaa ggtatgcctg agggtatggg
     4261 gccctatcct gctgtcaagc aatatcgccc tcgcatattt tactactata cgtcttgccc
     4321 ccctgtctgt caaccgtaag ccacccctgt tcaggaaaag ggccttgtct ttctttttca
     4381 ggataaccct ctcgatcatg taagtcttaa gagcttccag ggccttcctc cccacgggaa
     4441 caatccgctc ttttttcccc tttcctctca ccttcacaag cccctctcgg atgttcatgt
     4501 cttctgcatt cagtcctgac agttcaccaa ccctgagacc actggagtag agaagttcaa
     4561 gaatggccct gtcccgcgcc gggagaaatc ctattccttc cggcttctcg atgagcgaaa
     4621 agacatcatc cacagtgagg aacttgggga ggagtttcgg catcttcgga gtattcacga
     4681 ggcgtgccgg atttgtcttt atgataccct ccctgtgcat gaacctgaag aatgaccgca
     4741 ccgtggccag tctcctgccc actgtactct tcttaagacc cttgcccatc tgcgctgcca
     4801 caaagcctct cacatccata aaatcaacag actccacgtc agaatgaatc tcctcggaaa
     4861 atacatccag gtctttacga tacgacctta tggtgtgcgc tgaggcgccc ctttcagtgg
     4921 caaggtatct caaaaatttt tcgatctcct ctttcatgcg cccacctgtt caagataggc
     4981 cgaccagtcg gatatggccc tttccacaat catcctcctt ttcagttcct tcttctttat
     5041 gcctgagttc actgcaggga agagaccaaa gtgtacgttg cttggctgga aattcttggt
     5101 gggtaatgtg atatggtgaa tcagagaacc atgagcagtg gtcaccggtg tctccacaga
     5161 ttccttcccc cttatcttcc ttgaggcatt tatgcctgcc actagaccca ttgcagtgga
     5221 ctctatatat ccctcgaccc ccgtaatctg gcccgcgagg tataccgcag ggcaggcctt
     5281 taatgtcaaa tccctttcca gtgattccgg agcattgata aacgtgttcc tgtgcacact
     5341 gccatacctg agaaactcgg ccttttcaag tccggggatc atgctgaaga ccctcttctg
     5401 ctcaggccat ttaagccggg tctggaaacc caccatatta tatgcagacc tctctctgtt
     5461 ttcaggtctc aactgcacaa ccgcgtaagg ttcccttccc gtcttcggag ccggaaggcc
     5521 cacgggcttc atggggccga acctcagcgt gtcctttcct ctcagtgcca taacttccac
     5581 aggcatgcag ccctcaaaga ccttttcatt ctcaaagacc ctgatgttta ccctgtcagc
     5641 ctctgacagg gcaccgaaaa atgtctcgta ttcgtccttt gtcatggggc agttaatata
     5701 gtcatcgccg ccctttccgt atcttgaccc aaaatagacc ttcccgtacc cgatgctctc
     5761 cgcgtcaatg atgggcgcga tggcgtcata gaaatggaga tactcatgcc ccagaagttt
     5821 cttcagggat tctgccatag ggccagatgt caagggtccg gtggcaagaa cagagggcaa
     5881 atcctgtaat gcactcactt cttcccggat gatagtgatt agagggctgt cagagagggc
     5941 gttagtcagg aattccgcaa atgctcctct gtccactgcg agggcacttc ctgcagggat
     6001 cctggatgca actgctgcct tcataatgag agattcagcc atttcaagct ccttcttcag
     6061 gaggccgggt gcgctcagaa tatcagcaga gcgcaacgaa ttggaacaga ccagttctcc
     6121 cagcagccct gtcttgtggg cttcggtaag cctcgcaggt ctcatctcga agagcgtcac
     6181 ctcaactccc ctccttgctg cctgccatgc agcctcgcat cctgcgagcc cgccgccaat
     6241 aacaatcaat ccggccattg atttatttta gttttctcca ctagttcttg caaggcggca
     6301 attcctggga tttcccgcaa aaagaaggcc tgccctgtcc tttgtgagaa ttctcttgga
     6361 ataacccttg gaaatgggtt ggataatttc ttcaggcggg ggtatactgt gattatggaa
     6421 ttatgcccgg tttgcgaaca accttcaggt caatgcatct gttgtcctga gtgagggcac
     6481 gtatgtctcc tggatctggg agaaccatac tgtcctgtat gcaaaccagc ccccctgcaa
     6541 accaccatct ggcagcaaga aagacgatag taaatgaagt tccctatggt ataatctggt
     6601 atgctttcaa aatgggtgta tcccttgtca ctgcttcttt ccttcaccct cttcccctca
     6661 tccctttttt caaaagagac gcctcctgag gtaatggtca tcacgcttga tggcgttatc
     6721 aatcctgtgg cctctgaatt cataagcaag agtataaaaa aggccaccaa gaggggcgtt
     6781 gaggccctgg tggtcgagct cgataccccc ggcggcctgg atacctccat gagaatgatc
     6841 gtaaaggaga tcgtggggag tgaggtgcct gtagtggtct ttgtcgcacc cagcggcgca
     6901 agggcggcct ctgcgggtgt ttttattacc attgcagcgc atatcgccgc aatggctccc
     6961 ggtaccaaca tcggggcagc gcacccggtg gggcttggtg aaaaaatgga taagacaata
     7021 gcagaaaagg cgacgaatga tgccgcagca tatatcaaat cacttgcaga gagaaaggga
     7081 agaaatgcag cgtgggcaga aaaggcggtg agggaaagca tatctgccac cgaggttgag
     7141 gcattaaggg accatgtaat tgacatagtg agcaaagatc tcactacact cctcgttgac
     7201 attaatggca ggaagatcca gaccgctctt gcggagaagg ttctcaggac aaaagacgcg
     7261 aagataacac gggaagagac gggattgagg cagaagatac tgaatttcat cagtgatcct
     7321 aatgtggcct atatactgat gctccttggc ttctacggtc tcttttttga actcaccaac
     7381 ccgggtgcca ttttcccagg cgtgatcgga ggcatatgcc ttatcctcgc gttttacgca
     7441 ttccagacgc tgccggtaaa ctacgcaggc ctcctcctca tcatcctcgc catcattctc
     7501 tttattctgg aggtgaagat tgtttcccat ggggtattga ctattggggg tgtcatcgcc
     7561 atgattatag gttccctcat gctctttgag acgccgggtc cttttgtgag attgtcgctc
     7621 tatctcatac tccctgcggt aatggttacc gccctctttt ttagccttac catcgggctt
     7681 gcgctcaaag cacacaagag aaaaccagtg acaggaaagg aagagctcgt tgggatggaa
     7741 ggagtagcaa gaacagacat cactccggaa gggggcatgg cctctctcca cggagagcta
     7801 tgggccgcat actctgaaga gcgcattgca aaagacaata aggttgtggt tgaaagtgtt
     7861 tcgggactaa aactaaaggt tcacaaatca aaaggagggt aatatgggta atatgccaat
     7921 catagggccg tcgattctga gcatggtgtt tgtgatattt cttttcttct acttcctctc
     7981 cagcgccatt aagatactca aggagtatga gaggggtgtg gtcttcaggc tgggcaggat
     8041 aatccctatc aaaggaccgg gtcttgtgat catatggcct atcatcgaca agctcgtgag
     8101 ggtcagtctc agaacagtca ccttcgatgt cccttcacag gatataatca caaaggacaa
     8161 tatcacggtg aaggtcaatg ccgttgtcta cttcagggta atcgatccta tcagggcgat
     8221 cactgcggtg gaggactttt tctatgcgac ctcgcagatc gcccagacca ctctgaggtc
     8281 gatccttggg caaagccagc ttgacgacct cctcaccaag agggacgaac tcaatgcaga
     8341 gcttcagaag gtgatcgact tccagacaga gccctggggg ataaaggtgg ctaccgttga
     8401 ggtaaaaaac gttgaccttc ctgttgagat gcagcgggcc atcgcaaagc aggcagaggc
     8461 tgagagggaa agaagggcaa aggtcatcca tgcagacggc gaatttcagg catcacagaa
     8521 gcttgcagat gcagcacaga taatggcaac acagccgatg gcgatgcagt tgagatacct
     8581 tgggaccctg actgaaattg cgagtgaaaa gaattcgacc ataatattcc ctttgcctat
     8641 cgacctgatt aaaccattgt tcgacaggct ggagaagaaa gaacaatagg aggatacatc
     8701 aattacagag cacagcaaac tcagaacaaa gaaccgactc agattccttg tacttctcat
     8761 catactcgga tttctactca tatgggcttc gtacgaccta gggatatttc acctccttat
     8821 taaaattaag aaattctttt ccagcgaaga aaggatgcgg gcctttgttg attcatttgg
     8881 tccccttggc ttcatggctt tcattctctt gcaggtgact caggtggtcg cagcacccat
     8941 accgggtgaa gtcacgggac tgttgggagg cttcctctac ggtcccgttc ttggcgtagt
     9001 cctttccacc attggtctca ctatcggttc gtatgttgcg tttgccctcg cccgcgtttt
     9061 cggaagaccg tttgtggaca agtttgtgga caagagggta atgaagcgct ttgattatct
     9121 cctccatcac aagggcgcgt tcctggtctt tctcctcttt ctcataccag gctttccaaa
     9181 agactatctt tgctatatcc taggcctggg gcatctgtcc acaatggaat tcctcgtcat
     9241 cggcggaacg ggcaggcttt tcggtacgat ccttctcacc ctcggcggct cctacatacg
     9301 gcatcatcag tatatgagat tttttgtcct cgccggggtt gcaataatcg tcgtactcct
     9361 cgccatggta tatagggaca ggattgaaag gctctttaga aagtggcata tattgaaata
     9421 caaaaaaacc aagaaaaaaa aggcacaaca gaaccaaaca atgtcggcaa aataattatc
     9481 tgcaaagacc tcggcctgaa gagctcgtag tctgacgctc atcgcagtcc gtcttgcatc
     9541 attttccgtc attatcggga gaatgtcgag gtccttcgtg gccggcaata tcgcgcacag
     9601 tgcacaaaaa gcctttcctc gctgcatgtg caagaatgca ctgctaaacc aatagagtct
     9661 ccaaagctct gcgaacactt tcgaagccaa aaacattact ctattacatc ctttcctcct
     9721 gcaaaaaaag cctaaagaaa agcaagaaat gtatagaaat tagttctggg tagtgtcata
     9781 cttatagaaa tagccatgaa gaagcactgg aagtcaaacg tgctaagtat taagaacaag
     9841 agaatttctg gcgagtctct gaaacaaatc ctttctaata aaatcacgaa tctttttcat
     9901 gcaatgggag gtgataggaa tttacgtatt tcgcaaatga ccgaattata cataggtgag
     9961 ggaatgcgta cagcttttgc catgatatgt cacg
//
LOCUS       DATKOU010000085         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3566688, whole genome shotgun sequence.
ACCESSION   DATKOU010000085 DATKOU010000000
VERSION     DATKOU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3566688"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            730..1200
                     /locus_tag="VMU21_03940"
     CDS             730..1200
                     /locus_tag="VMU21_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76708.1"
                     /translation="MEHIERTMQLCRGELTHVVHYAHTLKEEIDRVSGFIQLNTLVVC
                     PACEKVCCINKHGYYDHEDLIYVFSLGQERPVYGKGIDDTAPCQFLSGQGCSRERSVR
                     PFRCNWYFCDTLLAHMEQGPAKPYREFITRFQGIIETRQKMMDEFSLITRFRAD"
     gene            complement(1422..2042)
                     /locus_tag="VMU21_03945"
     CDS             complement(1422..2042)
                     /locus_tag="VMU21_03945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO76709.1"
                     /translation="MDELAREYVISFYDRALHFFGDRPESLRWTPEGQRARYESMLDM
                     AHGIEGKKVLDFGCGKGDFYQFLKDKGIESHYTGVDINENLILMARQKFPKARFEVFD
                     IERDTMGEEFDYIFLCGVFNLRVQGIDELTRFTLKKLFPCCRIGLAFNGLSAHNPKKD
                     FELHYTSPEALFQFAVETLSPFVALRHDRIPYDFTMFVYRDLNSKP"
     gene            2197..2739
                     /locus_tag="VMU21_03950"
     CDS             2197..2739
                     /locus_tag="VMU21_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HUO76710.1"
                     /translation="MQLAHRLKRSSRNITRIALLCIIGTLCWSGLEHASATLASVDKV
                     VVIKSKRILMLLKDGEIFKTYRVSLGKRPEGHKIASGDQRTPEGRYILDSKNPNSKYH
                     LAIHISYPNELDIINAQKRGVSPGGEIMIHGLPPKRSNELKEINGYSDWTNGCIALTN
                     PEMEEIWQFVATGTPIEIKP"
     gene            complement(2760..>3667)
                     /locus_tag="VMU21_03955"
     CDS             complement(2760..>3667)
                     /locus_tag="VMU21_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HUO76711.1"
                     /translation="DGTSLPMKQLLSDDCRNGLVLITIRGRELPMVTLANGYKPKEGE
                     EVVVMGRTSGGNSTSSEGRIRSVRKQDGIFQVTVPLTPAGDGSPVFNRKGEVIGLSTL
                     IHGKKQAQHIVFPSRYVSKEFALYGDLTKGLSVLPPAEPLNKREEPPGTRLSDMAEQE
                     FLRGVSYEKSGMYEEAIAAYARALGINANYSDACVNLGLVYYRLGRYSKAVDAYKDAI
                     RINPAIPSVYNKLGAAYIILGEYPLALDAFKQAITIDPRSSDTHFNLGVAFILSGDKN
                     GALEQYILLKELDKHQAEKLLNLIP"
BASE COUNT          936 a          905 c          920 g          906 t
ORIGIN      
        1 acatggcgga tgacaaaacc tcagcctcct gcatctgaca tctccggcag ctggtccgtc
       61 ttccctaccg tacagggtgt ggagcaaggg cctgtctgtg agacctttac ccaggccggc
      121 aatttcctca ctttttctgg cgtcgctaat gggcttggca ttataagcgc gagcaatatt
      181 cagttggttt ttgtctcaaa tgttgagaac tgccagatag caactgaagt cacaggcacg
      241 cttacttctg cgagtactat aagcggaaat tatactcaga agggtaattg cgggacgtct
      301 ccaggcgaca aatggagggc agtaaaagga tgctcgtcta ctcagcttcc aagaggtttc
      361 ccggatgtcc ctctagggaa ctataacatt actgttactg cctgtgtttc agacacgtgt
      421 gcaagtagtc aaccttttcc attgaccaac gctgacatca atcaatttgc gcaggccctg
      481 ttgaatgcat tgaattcgga tataaaccag tcatggggca gcagttgcca ggcatcggga
      541 tgtagctgca cctctgccgt gaccagttat accccttgga atggcacatc tttcacgata
      601 actgataatt tttccattgt gtgcgccagt gggacccaaa ctgtgtcgtt gcagttcacc
      661 gtcacaaaga taacataaat aaggtcttcc aacatgctcc ctcatctcca agagcatgaa
      721 gcactgacgg tggaacacat tgaacggacg atgcagttgt gcagaggcga gttgacccat
      781 gtggtgcact atgcacacac gctcaaagaa gagatagaca gagtaagcgg ctttatccag
      841 ctcaatactt tagtggtatg tcccgcatgt gagaaggtct gctgcataaa caaacacggc
      901 tattatgacc atgaggatct tatctatgtc ttttccctgg ggcaggagcg cccggtctat
      961 ggaaagggga ttgacgatac tgctccgtgc cagtttcttt ccggacaagg ctgcagcagg
     1021 gaaagatcgg tgagaccatt caggtgcaac tggtattttt gcgataccct tctcgcacac
     1081 atggaacagg gccctgcaaa accctaccgc gaattcatca cccgctttca gggaataatc
     1141 gagacgagac agaagatgat ggatgagttt tccttaatca cccggttccg ggctgattag
     1201 tcttgaccat tcataagttt gacagaggtg tccggctcgg ctggcaatcc gcctaaggcg
     1261 gaagggttat gtgcgtgtcc gcaagggcgg accttgacaa tacaatacat aagaacaaag
     1321 tgttgttagg tgtatagacc tcgctccagt caaggagggc cattgtcttg cccggagaca
     1381 gacgcctctg tcgaagcaaa aaaactcatg aattatctgg gttagggttt cgaattcagg
     1441 tccctgtaca cgaacatcgt gaagtcgtaa ggaatcctgt catgcctcaa tgccacaaag
     1501 ggggagaggg tctcgaccgc gaactgaaag agcgcttccg gtgaggtgta gtggagctcg
     1561 aaatcttttt tcggattatg ggcagagagg ccgttaaagg cgagaccaat cctgcagcag
     1621 gggaagagtt ttttgagggt aaacctggtg agttcgtcga ttccctggac cctgaggttg
     1681 aataccccgc aaagaaatat gtaatcaaac tcttcgccca tagtatccct ctctatgtca
     1741 aagacctcaa acctggcttt agggaacttt tgccttgcca tcagaataag gttttcgttt
     1801 atgtcaacac cggtgtaatg cgactctatc cccttgtcct tcaggaactg ataaaagtcg
     1861 cccttgccgc acccgaaatc aaggactttc ttcccctcta tcccatgggc catatcgagc
     1921 attgactcat aacgcgcacg ctgaccctcc ggagtccatc gaagggattc gggcctgtct
     1981 ccaaagaaat gaagcgcacg gtcataaaag gagatcacat actccctggc aagctcatcc
     2041 atgaattagt ataccaccat ggtcgcggta atgaggaaca ggctgccccg tactaagcat
     2101 aaattaagtt ttgttaattt ttttcttgac aagaaaggct tttattatta atatgttacg
     2161 tagaagaaac tcttgagagg aggctgtcgg catacaatgc aacttgctca cagattaaaa
     2221 agatcatccc ggaacattac aagaatcgcc ctgctttgta tcatcggtac cctatgctgg
     2281 tccggacttg agcatgcatc agccacgctg gccagcgttg acaaagttgt ggtgattaag
     2341 agcaagcgga tactcatgct tttgaaggat ggagagattt tcaagacgta cagggtctct
     2401 ctgggaaaaa ggccggaagg ccataaaata gcatcaggtg atcagcggac acctgaagga
     2461 aggtatatcc tggattcaaa gaacccgaac agcaagtacc atctggcaat ccatatttcc
     2521 tatcccaatg aattggacat catcaatgcc cagaagcgcg gggtctcccc cgggggggaa
     2581 atcatgattc atggattgcc gcccaaacgc tccaatgagt tgaaggaaat aaacggatat
     2641 tcggattgga ccaatggatg cattgcgctc accaatcccg agatggaaga gatctggcag
     2701 tttgttgcca ctggtactcc catagaaatc aagccgtaag gcgcctcact gctcattact
     2761 tacggaataa ggttcagcag tttttctgcc tggtgtttgt ccagttcctt caggagaatg
     2821 tattgctcca gcgcaccgtt cttatcgccg gaaaggatga aggcaacccc gaggttgaag
     2881 tgcgtgtcag agcttctcgg atcaattgtg atcgcctgtt tgaaggcatc aagggccaat
     2941 gggtattcgc ccaggatgat gtacgctgcg ccaagcttgt tgtacactga cgggatggcc
     3001 ggattaatcc ttatggcatc tttatatgcg tccactgctt tggagtatct gccgagccgg
     3061 taatagacta atcccagatt cacacatgcg tcgctgtagt tggcattaat ccccagtgcc
     3121 cgggcgtatg cggcaatggc ctcttcgtac atcccggact tttcatagga aacgccccgc
     3181 aggaattcct gctctgccat atcagagaga cgcgtaccgg gaggctcttc ccttttattc
     3241 agaggctcag cagggggaag cacggagagt ccctttgtga ggtctccgta gagagcgaat
     3301 tctttcgaaa cataacggga aggaaatacg atatgctggg cctgtttctt cccatggatg
     3361 agggtggaaa gacctatgac ctcgcccttt cggttgaaca caggacttcc gtcacccgca
     3421 ggagtgaggg gaacggtcac ctgaaagata ccatcttgct tgcggacact tcttatcctt
     3481 ccctctgacg aggtgctatt cccccccgac gtccttccca tcaccaccac ttcttccccc
     3541 tcctttggtt tgtacccatt ggcgagtgtg accatgggga gttcacgacc ccggatcgta
     3601 atcaggacaa ggccgttacg acaatcatct gaaagcaatt gcttcatagg gagagaggtc
     3661 ccgtctg
//
LOCUS       DATKOU010000086         7332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5110466, whole genome shotgun sequence.
ACCESSION   DATKOU010000086 DATKOU010000000
VERSION     DATKOU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5110466"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..572
                     /locus_tag="VMU21_03960"
     CDS             357..572
                     /locus_tag="VMU21_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HUO76712.1"
                     /translation="MKYGARNNITATVKSVKKGDIMSLVKYDITQPTEMASVLTTESA
                     EDLELKPGDKVQLIIKAIHVLPAKEER"
     gene            611..1201
                     /gene="yedF"
                     /locus_tag="VMU21_03965"
     CDS             611..1201
                     /gene="yedF"
                     /locus_tag="VMU21_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HUO76713.1"
                     /translation="MIIDARGQDCPKPVMMAEEALSKITEGIVEVIVDNEASADNLAL
                     FAKSNAFSAKTIREGKDWRVKIVKGYSCAPAAGDRNVPETEQTLLLVVGTDTMGKEED
                     LGKILMKAYFETIKAYKQVPHTVFFLNAGVNLTTTNADIWPILREIEAMGVEMYSCGT
                     CLKHYGLENELKVGHRGTTNHIVEGMKDFDKTVWIG"
     gene            complement(1496..2650)
                     /locus_tag="VMU21_03970"
     CDS             complement(1496..2650)
                     /locus_tag="VMU21_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76714.1"
                     /translation="MVREIMIEFAKRTGLLPEASLPKRYLWTDAYAVCNFLGLYRQTN
                     NEQYKRFAFDLVDQVHATLGRHRRDDPRTGWISGLDEEEGRRHPTKGGLRIGKEMNER
                     RPEEPFNERLEWDRDGQYYHYLTQWMHALHCVTKVTGDPTFQRWAIELAKTAHARFTY
                     VPPGGGEKRLYWKMSIDLSYPLVSSMGHHDPLNGLVTYSELQSTAGKYPEGLPSPDLN
                     AEIAGMAVICEEKNWSTDDPLGIGGLLIDAYKMAQLIAADRFMRPDLLGTVLDDSLTG
                     LDYFARKSPFKLPAHYRLAFRELGLSIGLQAIERLQVLIEQYPDAFKKRHDVHSRIRN
                     LMEYVHLSELTNAFWVERTNRESSSWTEHREINMVMLATSLSPDGYLPPL"
     gene            2881..3648
                     /locus_tag="VMU21_03975"
     CDS             2881..3648
                     /locus_tag="VMU21_03975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUO76715.1"
                     /translation="MRSIAYLSLFACMLFFEARHPFAQSGQKKAFRIVFHSSISILNS
                     LILYLILFSPLYAAVRFTQLHAFGLVSALGLRGWTEVLATLVVFDCWDYWMHRANHRI
                     GLFWRFHKAHHSDMEIDVTTASRFHIGELLITNSIKGAVILLWGPSLLGIAAFETLLT
                     AASQFHHSNVNIPFGSQDRWEKVIVTPRMHRCHHVFAGMCQLSNYSTVFSVWDRLFGT
                     YHWAETTKELEPIGVLRPRGPETMEFIPLLLTPFQKG"
     gene            complement(3687..4271)
                     /locus_tag="VMU21_03980"
     CDS             complement(3687..4271)
                     /locus_tag="VMU21_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76716.1"
                     /translation="MSKTIRMHALTSALVALISALLFISLQSLAAEHPSEHPSGKPSS
                     GEVPKPLTKKEMSAAIQSYIEKEAKKYGGYYEIYDDLQKKQLSLKLKQIHDDRLSHVG
                     NDVYFFCVDLIDKDGKVYDLDFFMKRGPGGDLNVTEVTIHKQEGKERYTWYEEKGIWK
                     KKWLIQNPAQPAQGDKLPAGKSTIGDHPNPLPPR"
     gene            4485..4874
                     /locus_tag="VMU21_03985"
     CDS             4485..4874
                     /locus_tag="VMU21_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4749..4751,aa:Sec)
                     /transl_table=11
                     /product="rhodanese-related (seleno)protein"
                     /protein_id="HUO76717.1"
                     /translation="MKRAMLLAILGVLVMFIGCTNMQVPRILKEELKAKLGSPDLVLL
                     DVRAKNDWEESGEKIIDAKRMDPTTVDTWAGTLPKEKEIVLYCAUPREGTSARVAEKL
                     IAKGFNAKALKGGWKEWSEAKYPVEKK"
     gene            5008..5505
                     /locus_tag="VMU21_03990"
     CDS             5008..5505
                     /locus_tag="VMU21_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HUO76718.1"
                     /translation="MNAYEKEVVMPVQRLKEYLKRNNIKYVSIVHSEAYTAQEIAALA
                     HVSGKELAKTVVIKKDGQMAMAVLSASHKIDWNRLKAATGAVKVELAGEKEFRDMFPD
                     CEVGAMPPFGNLYKMEVFVETSLTRDEEIAFNAGSHIELIKLSFEDFSRLVKPKVASF
                     STSNI"
     gene            complement(5610..6044)
                     /locus_tag="VMU21_03995"
     CDS             complement(5610..6044)
                     /locus_tag="VMU21_03995"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HUO76719.1"
                     /translation="MRRGICVCTLMLALALAGICLAADEVSCPDTIKVNQKAMPPSPE
                     WDVSYSALSHQLEMVTFFSGHPRDNASLVYDKRSKTKGGWVGTWIFPKDAGGHWIRCS
                     YTGTRAELFRRLPISVSVCLVTYDEGSHFPSGLPVIRKIECR"
     gene            6461..7192
                     /locus_tag="VMU21_04000"
     CDS             6461..7192
                     /locus_tag="VMU21_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HUO76720.1"
                     /translation="MIEVYKNLPGTNCGKCGLSSCMAFALKVKKSQASLSECPFVTGE
                     TMDRGQPPSAASSFSSYEKVSRELEKEAVMVDFKDTAEIIGGMYEAVDGREAIMVKMI
                     STLYELRKEGLFQNNAPCGDVWAKIIICDYVRRKGRGPLTGELLPLGLFPHSASLVRA
                     FQSSAEKKIAEGFKRDLNGSKKRCGALGGRETEGKVKADYISCFDLLPHVPLYLSFWV
                     DDGEFDADCKLLFDRNAEKHTPVQQ"
BASE COUNT         1917 a         1663 c         1928 g         1824 t
ORIGIN      
        1 gatcctggca ggttttgtgc ctggcggtcc cggtattccg tatgccttcg aatatgcgct
       61 caagtgtctt gatattgagg caaaattcag gaaaggcgag aagtgggagt atctgcctgc
      121 tccgaaattt gaccacgggg tattctgagc ggcatcgtga ataaaggaat gaacaatccc
      181 gcaaagctct tcatagtggt gtgtgatgtc tcaaaggtcg gtacaggtgg agatctttgc
      241 gatggtgtct cactatgtgt tgaatatgcc gaagatagcg ttaattcgac ctgtgagaga
      301 gataagggag atgcatttca acaacatcaa aatcaaatat tgtgaggagg tctgttatga
      361 agtatggagc aagaaacaat attactgcga cagtaaaatc ggtcaaaaag ggagatatta
      421 tgtcgcttgt aaaatacgat atcacgcaac cgactgagat ggcttcggtc cttacgacag
      481 agtcggcgga ggatcttgag cttaagccgg gagataaggt ccagcttatt atcaaggcaa
      541 ttcatgtcct gccggcaaaa gaggagaggt gattttttta accatacagg tttaaaaata
      601 ggagattgtc atgattattg acgcaagagg ccaggattgc ccgaagcctg ttatgatggc
      661 ggaagaagcc ctgtcgaaaa taacagaagg cattgttgaa gtcattgttg acaatgaagc
      721 ctcggcagac aatcttgcat tgttcgcgaa gagcaatgct ttttctgcca agacaatcag
      781 ggaaggtaaa gactggcggg tgaagatcgt gaaaggatat tcatgtgctc ccgcggccgg
      841 tgacaggaat gtaccagaga cagaacaaac cttgctgctt gttgttggca ctgatacgat
      901 gggaaaagaa gaagatctcg gcaaaattct gatgaaggcc tatttcgaaa ccataaaggc
      961 ctataaacaa gttccccata cagtcttttt tctgaatgcg ggcgtgaacc ttacgactac
     1021 aaatgctgac atctggccga tcctaagaga aatcgaggcg atgggtgtcg aaatgtattc
     1081 ctgtggaaca tgtctcaaac attatggact tgagaacgaa ctgaaggtgg gccacagagg
     1141 aacgacaaac catattgtag aaggcatgaa ggattttgat aagacagtgt ggataggtta
     1201 aaggattaac acgtctccgt cggcctctca agtgactccc tgtttatcag accctgttta
     1261 cagatatttc acatgtgcgg cgttctacac ttaaccttac tctccgtcca atcggttcgc
     1321 cgagtatttc tacttgtaca tctcaccttc ggcaaaaaac ggtcagtttc gattacagga
     1381 agaaaacgaa aaagaactct taactgtttg gatggaaaac cgcaacagtt gtaacatcca
     1441 acacctatct acatagcagg tggagatgat attccagact gaggtagggg atcagtcata
     1501 gaggcgggag atacccatcg ggagacaggc tggtagcaag cataaccatg ttgatctcgc
     1561 gatgctctgt ccagctgctg gattctctgt tagtgcgctc aacccagaat gcattggtca
     1621 gctcactcag atggacgtac tccatgaggt ttctgatacg cgaatggaca tcatgccgct
     1681 tcttgaaggc atccggatat tgctcaatca acacttgaag tctctcaatg gcctgtaatc
     1741 cgatcgacaa tcccagctct cgaaatgcca gccggtaatg agcggggagc ttgaagggag
     1801 actttctcgc aaagtagtcc aatcctgtga gcgaatcatc caaaacagtc ccgaggaggt
     1861 ccggtcgcat gaagcggtcc gcagcaatta attgtgccat tttgtaagcg tcgatcagga
     1921 gaccgccaat gccaaggggg tcatcagtgc tccagttctt ttcttcacaa atcacggcca
     1981 tgccggcaat ctccgcgttg agatctggcg agggtaatcc ctcaggatat ttccccgcag
     2041 tcgactggag ttcgctgtaa gtgaccagtc cgttaagagg gtcgtgatga cccattgacg
     2101 acacaagcgg atacgagaga tctatactca tcttccagta caggcgtttt tctcccccgc
     2161 ctggtggcac gtaagtgaac cttgcatggg ctgtttttgc gagctctatc gcccaccttt
     2221 gaaaggtcgg atcccccgta accttggtca cacaatggag ggcgtgcatc cattgtgtca
     2281 ggtaatgata atactgcccg tcccggtccc attccaggcg ctcattaaat ggctcctccg
     2341 gtcgccgttc gttcatctct ttccctattc tcagcccccc tttcgtgggg tgcctgcggc
     2401 cttcctcctc atccaggccg ctgatccagc cggtacgagg gtcatccctg cggtgccggc
     2461 ccagcgtggc atgcacctga tcgaccaagt caaaagcgaa acgcttgtac tgctcattat
     2521 tcgtctgacg atagagccca aggaaattgc agaccgcgta agcatcagtc catagatagc
     2581 gcttcgggag agaggcctca ggcaagagtc cggtcctctt cgcaaattcg atcatgattt
     2641 cgcgaaccat ataatccctt atgtatctat aataatcaga tacaatgaaa aggctcaagg
     2701 tccaaatgtt cgggaaggct ttgagattcc gggtgttggg ccgtgcggtg atagaggctg
     2761 cggctggatc cgggtgcgga cttacaggtg aacccagagt tgctaaaaaa gacgaactac
     2821 attcctctga gttgatccga atactggttg gactataata gaacgatgaa tccctttctt
     2881 gtgagatcaa tagcatatct ttcgctcttt gcctgcatgc tcttttttga ggcacgtcat
     2941 ccttttgcac agtccgggca gaagaaagct ttccgcatcg tctttcactc cagtataagc
     3001 attctcaact ccctcatcct ctatctcatt ctgttttctc ctctctatgc tgcagtccgt
     3061 tttactcaat tgcacgcctt tggtctcgta tcggctttag gtctccgtgg ctggacagag
     3121 gttcttgcaa cccttgtggt ttttgactgc tgggactact ggatgcaccg tgcaaatcac
     3181 aggatcggtc ttttctggag gtttcataag gctcatcatt ctgacatgga aatagatgtg
     3241 acgacagctt caaggttcca tattggcgaa ctcctgataa caaatagtat aaagggtgcg
     3301 gtcatactcc tatggggacc ttctctcctg gggattgcag cctttgagac gctactcacc
     3361 gctgcatcgc aattccacca cagtaatgtg aatatcccat tcggttcgca ggacagatgg
     3421 gaaaaggtca ttgtcacccc gaggatgcat cgctgccacc atgtctttgc aggcatgtgt
     3481 caactgagta actattcgac tgtcttcagt gtatgggaca ggttattcgg gacctaccat
     3541 tgggccgaga caacaaaaga gttggagccc atcggagtgt tgagaccgag gggaccggaa
     3601 acaatggaat tcattcctct acttctgacc ccctttcaga aaggttagtt cctcttgccg
     3661 ctctgtatct gttccggatg atttccttaa cgagggggca gcggattggg gtgatcgccg
     3721 atagtcgatt ttcccgcagg cagtttatct ccttgagcgg gctgagcagg gttctgaatt
     3781 aaccacttct tcttccagat gcccttttcc tcataccatg tgtatctctc ttttccctcc
     3841 tgcttgtgga tcgtcacctc ggtgacattc aggtcaccac cggggcccct cttcatgaag
     3901 aaatcgagat catagacctt cccgtccttg tcgatgaggt caacgcagaa aaagtagaca
     3961 tcgttgccga catgggaaag acggtcatcg tggatttgct tcagtttgag agaaagttgt
     4021 ttcttttgca ggtcgtcata gatttcgtaa tatcctccgt attttttcgc ttctttctca
     4081 atatacgact ggattgctgc tgacatttct ttcttggtta aaggtttcgg cacttcccct
     4141 gaagaaggtt tccctgaagg gtgttcgctt ggatgttctg cggccaggga ctgaagggag
     4201 ataaaaagta gcgcagagat aagtgcgacg agagcggacg taagagcatg catccttatt
     4261 gttttagaca tactacctcc tcatgttctg tgagtaaaaa atacagtaaa atgctgtatg
     4321 tcattatagc acgatgaaaa ggggcattat aggaaggggg tgaccggcgt gatatagaaa
     4381 agaatgctgg cgcttgaata gaggcgtcgg tcggattaga atattcttag acaaaatgat
     4441 ttttaataag aaatgtaact gggacggaag atgagggaag acatatgaaa agagcgatgt
     4501 tgctcgcaat acttggagtt cttgttatgt tcattgggtg cacgaacatg caagtgcccc
     4561 gcattttgaa ggaggaactg aaggccaaac ttggatcacc cgatctggtt ttactggatg
     4621 tcagggctaa gaatgactgg gaagagagcg gcgaaaagat tatcgatgct aaacggatgg
     4681 acccgacgac cgtcgacacc tgggcaggca cattgccaaa ggaaaaggag atagtcctct
     4741 actgcgcgtg accgcgagaa gggacgagcg cccgtgtggc ggaaaagctg attgcaaaag
     4801 gctttaatgc aaaggccctt aagggcggct ggaaagaatg gtcggaggcc aaataccctg
     4861 tcgagaaaaa gtaattctta tcagggtcac atcccggttt ctcttcccca atgtctttcc
     4921 cggagaattc tttcgttgct ttcgctttga atgcacgagt gagtttgtct cagggacatg
     4981 gacgtccata gcccagaagt aagaggaatg aatgcttatg aaaaggaggt tgtcatgccg
     5041 gtgcaaagac tgaaggaata tctcaaacgt aacaacatca agtatgtatc tatcgttcac
     5101 tccgaagcgt atacggccca ggaaatcgcc gcgttggcac acgtttcggg aaaagaattg
     5161 gcaaagaccg tagttattaa aaaggacggt caaatggcga tggctgtgtt gtcagcctca
     5221 cacaagatag actggaaccg tttgaaggct gcaacagggg ccgtcaaggt tgaacttgcc
     5281 ggcgagaaag aatttaggga tatgtttcct gattgcgaag tcggggctat gccgcccttt
     5341 ggaaatctgt ataagatgga agtgtttgtg gaaacaagct tgacgagaga cgaagaaatc
     5401 gcatttaacg ccgggtccca tatagagctt ataaaactgt catttgagga tttctcgagg
     5461 ttagtgaagc cgaaggtggc gagcttttcg accagtaata tataaaggtc cctagtgaat
     5521 ctgccgaatg ttttgccgcc atccgcctcg ccaagcgaag cgacaaggag cagcaatgcg
     5581 gggataattc tgctaaggga aaaggatatc taccggcact caatctttcg tataaccggt
     5641 aaccctgaag gaaaatgcga cccctcatcg taggtaacca ggcagacact caccgaaatt
     5701 gggaggcgtc tgaagagctc tgcccgtgtt cctgtatacg aacaacgtat ccagtggccg
     5761 ccggcatcct tgggaaagat ccatgtgccg acccagccgc cctttgtctt cgatcttttg
     5821 tcatatacaa gggatgcatt gtcacgggga tggccgctaa agaaagtcac catctcaagc
     5881 tggtgggata aggcgctgta tgacacgtcc cactcaggcg atggaggcat ggccttctgg
     5941 ttaactttga tcgtgtcagg acacgatacc tcgtccgcag caaggcatat gccggccaat
     6001 gcaagggcaa gcataagtgt gcatacacat atacctcgtc tcatccccgg tccttcttgc
     6061 aaacgcctgc cgagtgatcc ataagtggaa ttcagccggc gggcgtctct tttcttgaat
     6121 cctagcctgt ggaaaacctg aagtcaagca aaacttcagt gcgatgcggc cgtgtgtctc
     6181 atcctgccaa tagaaatagt gcacagcttt tacagaaaag aattgctgcg cgtcacgttc
     6241 gattcctcaa tgtatcattc agatgcaaag gcaggcggca cttactgtgt gaagaattga
     6301 ggtcttcggg aagggtcaaa ctaaagtacc tgctgtcacc ggcgaagccc agggaattgg
     6361 acggtcggaa gcgggtaggc tggcctgcaa cccggcaccc tctgaaccgt catgatttcg
     6421 atcataggtg cgaccgggtt ttccttgcta caataagctt gtgattgagg tatataagaa
     6481 ccttccggga acaaactgcg gcaagtgtgg cctgtcttca tgtatggcct ttgcactgaa
     6541 ggtgaagaag tcccaggctt ccctgtccga atgtcccttt gtgacgggag agacaatgga
     6601 taggggacaa ccgccttctg ccgcgtcgtc tttcagcagt tatgagaagg tgagccggga
     6661 actcgaaaaa gaggcagtta tggtggactt caaggatacc gccgaaataa tcgggggaat
     6721 gtatgaagcc gtggatggca gggaagcgat catggtgaaa atgatcagta cactgtacga
     6781 actgcgtaaa gaggggttat tccagaataa tgcgccatgc ggggatgtat gggcaaagat
     6841 cattatctgt gattacgtgc gcaggaaggg aagaggcccg ctgacagggg agttgcttcc
     6901 cctcgggctc ttccctcact ctgcctctct ggtgagggcc ttccaaagca gcgcggagaa
     6961 aaagattgct gagggcttta agagagacct gaatgggtcg aaaaagcgtt gtggagcact
     7021 ggggggcaga gaaactgaag gaaaggtcaa agctgactat atctcctgtt tcgacctttt
     7081 gccgcatgtg ccgctctatc tctcattctg ggttgatgat ggggagttcg atgcggactg
     7141 caagcttctg ttcgacagga acgcggaaaa acatacgcca gttcaacaat gaagtgcaac
     7201 acttgggaga gcaggaaagg agatgctatc ctaagtgtct atgccaaaga actacaaaca
     7261 tcttaccatg gacgaaagag aagtcatagc caaaatgcac tgggagggta aagggctgag
     7321 cgagatcgcc aa
//
LOCUS       DATKOU010000087         4004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3573559, whole genome shotgun sequence.
ACCESSION   DATKOU010000087 DATKOU010000000
VERSION     DATKOU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573559"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VMU21_04005"
     CDS             complement(<1..477)
                     /locus_tag="VMU21_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HUO76721.1"
                     /translation="MANNPIITLTTDFGYRDPLAGIMKGVILSINPDVNIVDITHGIS
                     KYNVRESALTIGMSYHEFPSSTIHVVVTDPGVGSERRPIMVVTEDYYFIGPDNGVFSI
                     VYSESERCEVLHLTAEHYFMRDKSATFHGRDVFAPVASWLSKGVISSKFGDPVTDFV"
     gene            complement(516..1427)
                     /locus_tag="VMU21_04010"
     CDS             complement(516..1427)
                     /locus_tag="VMU21_04010"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HUO76722.1"
                     /translation="MDLSVEIGKLKLKNPVMTASGTFGYGEEYSEFMDLNRLGAVVVK
                     GLSLLPKEGNPPPRIVETAGGMLNAIGLQNIGIERFIQEKLPFLKQFNTPVIVNFFGD
                     SVEEYAKAAERLSTSGGIHGLEMNISCPNKQAGWCIFGTDPKVTFEVVSAVRKSTDLI
                     LIVKLSPNVTDIGVMARAAEDAGADAVSLINTITGMAIDIETRRPKLANITGGLSGPA
                     VKPVALRMVWEVYRAVKVPIIGMGGIMNGRDSIEFILAGATAVAVGTAHFINPVTSVQ
                     VIEGMEDFMREKGVSHIKNLIGGLITE"
     gene            complement(1644..2453)
                     /locus_tag="VMU21_04015"
     CDS             complement(1644..2453)
                     /locus_tag="VMU21_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HUO76723.1"
                     /translation="MSRYFTAEIAENRPLNNDFHLLTFFPLGHTKEPQPGQFYMLSTL
                     GKSRAGSSFEEIHDPLLKRPFSLFRKTGKGLQILFRIRGRGTAMMKELKKSSLVNVLG
                     PLGNSYPFPAGNKTPLIIAGGIGIASLFYFAERLSKAKKKAYISYGVRSENELFMTDE
                     VKRFAKELFISTDDGSCGERGCVTDMVGGLLSRIPSLGTNSVIYACGPRKMLEAVSRM
                     AKQKGIEAYLSMEEVMACGVGACLGCVVKTVSGYKRVCKEGPIFDAKEIVW"
     gene            complement(2446..2961)
                     /gene="greA"
                     /locus_tag="VMU21_04020"
     CDS             complement(2446..2961)
                     /gene="greA"
                     /locus_tag="VMU21_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HUO76724.1"
                     /translation="MLNYFSLDEGGEVMQMRVPVTPEGFLKLQEELERLIKVERPKNI
                     QAIAEARAHGDLSENAEYHAAKERQSFLEGRIQELQAKVALAEIIDPSKINQSKVGFG
                     AKVKVLDIEADEEYTFTLVGAEEADVKQGKISISSPVGRALLGKEIGDTVTIKAPART
                     MEYEILEITFE"
     gene            3133..>4004
                     /gene="proS"
                     /locus_tag="VMU21_04025"
     CDS             3133..>4004
                     /gene="proS"
                     /locus_tag="VMU21_04025"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HUO76725.1"
                     /translation="MRFSKLLIPTLRETPSEVEAISHNLMLRAGYVRQLAAGLYVFLP
                     LGWRVLHKIHAILREEMDAIGGQEISMPVLHPAELWQQTGRWDAIGDEMFRLKDRTGR
                     DMCLGMTHEEIMTWLASREIRSYRDLPQTWYQIQTKLRDEARPKSGVLRTREFIMKDS
                     YSFDASNDGLAKNYQLHAEAYHRIFTRCGLKFYQVESDPGMMGGATSHEFMAPSPAGE
                     DDVALCAQCGYAANIELARSIPLKNEPKDWQLEEVNTPDKRTVQEVSNFLKLPPSYFI
                     KSILVMNDKVPVLAL"
BASE COUNT          961 a         1119 c          904 g         1020 t
ORIGIN      
        1 cacaaagtcg gttaccggat caccaaactt cgatgaaatg actccctttg agagccacga
       61 agccacagga gcgaagacgt ccctgccgtg aaacgttgca ctcttgtcac gcatgaaata
      121 gtgctctgca gtgagatgga ggacctcgca gcgctcactc tcactgtaga caatggaaaa
      181 cacaccgttg tcgggaccga tgaaatagta gtcttcagtt actaccatga taggtcttct
      241 ttctgagcca acaccggggt cggtcaccac tacatgaatg gtgcttgagg ggaactcatg
      301 atagctcatc ccgatggtga gggcagactc cctcacgttg tatttactga ttccatgggt
      361 aatatcgacg atattgacat ccggattaat actgaggatg acccccttca taattccggc
      421 cagagggtcc ctatacccga agtctgtggt gagggtaata atgggattat tcgccattgc
      481 atacctccga ataattcttt gctaaagagc gcttttcact ctgtgatcaa accacctatg
      541 agatttttta tatgggagac ccctttctcc ctcataaagt cttccattcc ttcaatgacc
      601 tgaacagaag tgactggatt tatgaaatgc gcggtgccca ccgccactgc tgtggcccct
      661 gcaagaatga attctatgga atcgcgtcca ttcattatcc cccccatacc aataatgggg
      721 accttcaccg ccctgtaaac ctcccacacc atcctcagtg ccacaggctt caccgcaggt
      781 ccggacaggc caccggtaat atttgcaagt ttcggtcttc ttgtctcgat atcgatagcc
      841 atccccgtaa tggtgtttat gagggaaacc gcatcagcac ccgcatcctc agctgcgcga
      901 gccataaccc cgatatccgt cacattgggc gagagtttta cgataaggat aaggtccgtg
      961 gattttctga ccgcgctcac cacctcaaag gtcaccttgg ggtcggtacc gaaaatacac
     1021 cagcccgcct gcttgttcgg gcatgatatg ttcatctcga gaccgtgtat tcccccgctt
     1081 gtactgagcc gctctgccgc ctttgcatac tcttccacac tgtccccgaa aaaattcact
     1141 atcaccggag tattgaactg tttaaggaag ggaagtttct cctggataaa gcgctctatc
     1201 ccaatgttct ggagcccgat agcattgagc atccccccgg ccgtctccac aatgcggggc
     1261 ggaggatttc cttccttggg caggagagag agtcccttca ccaccaccgc acccagtctg
     1321 ttcaggtcca tgaattccga atactcctca ccgtaaccga acgttcctga cgcggtcatc
     1381 accggattct tgagtttcag cttccctatt tcaacactta agtccatgta tcctgccccc
     1441 ggtcttcaca cccttcgcta caatcaggga tgtggggtga taaagtttta acgataagtt
     1501 ttggccaact acattacaaa tctcatttaa caaacgacaa agccgtttgt taagactgcg
     1561 aggaatatct gccgcaacat ttcgcatcgg ttgtaaaatt attgcattta agacttttca
     1621 atcccgaatc cctgattttc tatctaccac acaatttctt tcgcgtcaaa tatcggccct
     1681 tccttgcata cccttttata cccgctgacc gtcttcacca cgcacccgag acacgcaccc
     1741 actccgcacg ccataacttc ctccatcgag aggtacgcct caatcccctt ctgttttgcc
     1801 atccgtgata ccgcttcaag catctttctc ggaccgcatg cataaatcac gctattggtg
     1861 cccagcgagg gaatacggga aagaagaccg cccaccatat cggtgacgca tcccctttca
     1921 ccgcaggagc cgtcgtccgt gcttatgaag agctctttcg caaatctctt cacctcatcc
     1981 gtcataaaaa gctcattttc ggaccgtacc ccataggaga tgtatgcctt cttcttcgct
     2041 ttcgagagcc gttccgcgaa ataaaacagg gaggcgatcc cgatgcctcc cgcaatgatc
     2101 aagggggttt tgttccctgc tggaaaggga taggaattcc ccagcggtcc caggacgttc
     2161 acaagcgaac tttttttcaa ctccttcatc attgcagtcc ctctgcccct tatcctgaag
     2221 agtatttgga gcccctttcc ggttttcctg aaaagactga atggcctctt cagaagtggg
     2281 tcatggatct cttcgaagga tgaacctgcc ctggacttcc cgagagtact aagcatgtag
     2341 aattgaccgg gttggggctc ttttgtgtgt cccagaggaa agaaggtgag gaggtgaaaa
     2401 tcgttattga gcggcctgtt ttctgctatc tctgcagtga aatatctact caaaggttat
     2461 ctctaagatc tcatattcca tggtccttgc aggcgccttg atggtcaccg tatccccgat
     2521 ctcttttccg agcagcgccc tgcccacagg agagctgatg gatatcttcc cttgctttac
     2581 atccgcctcc tccgcaccca caagggtaaa ggtatactcc tcatccgctt cgatatcaag
     2641 gaccttgacc tttgcaccaa agccgacttt ggactggttt atctttgacg gatcaatgat
     2701 ctcggcaagg gctacttttg cctggagctc ctgtattctt ccttccagaa aggactgcct
     2761 ttccttggca gcatgatatt ctgcgttctc cgaaaggtca ccatgcgcac gggcctctgc
     2821 aatggcctga atatttttcg gcctctccac cttgataagc cgctcaagtt cctcctggag
     2881 tttgagaaaa ccctcagggg tcaccggtac tctcatctgc atcacttctc ctccctcgtc
     2941 aagactaaag taatttaaca ctttcgccgc atttttttaa agttcccttg actattgcct
     3001 gcgtattcat tactctaatt tacgatagtg gatagtttta aatgagcagc ggcctgccct
     3061 ctcctttccg ttcatattcc tatcactatt cactatgcag ttttcactca caactgaatt
     3121 tgaggaggac acatgcgttt ctcaaaatta ttgataccca ccttgaggga aaccccttca
     3181 gaagttgagg caataagcca taacttaatg ctcagggccg gatacgtccg gcagctcgcc
     3241 gcaggcctct atgtctttct tcccctgggc tggagggttc ttcacaagat acacgcgatc
     3301 ctcagggaag agatggatgc cataggcggc caggagatat ccatgcctgt gctccatcct
     3361 gcggaactat ggcagcagac agggagatgg gacgccatcg gtgatgagat gttcaggctg
     3421 aaggacagga caggtaggga catgtgtctc ggcatgaccc acgaggagat catgacatgg
     3481 ctcgcatcaa gagagattcg ctcgtaccgg gacctccccc agacatggta ccagatacag
     3541 acaaagctca gggacgaggc gagaccaaag agcggggtat tgcgtacaag ggaattcatc
     3601 atgaaggaca gctacagctt tgatgcatcg aacgacggcc tcgcgaaaaa ctatcagctc
     3661 catgcagagg cataccacag gattttcacc cgctgcggcc tgaaattcta tcaggtggag
     3721 tctgacccgg gcatgatggg aggagcaacc tcgcatgaat tcatggcccc cagccctgca
     3781 ggggaagatg atgtcgcact ctgcgcccaa tgcggatacg ccgccaatat agagcttgcg
     3841 cgctcgattc ctttgaagaa tgaaccgaag gactggcaat tggaagaggt caatacccct
     3901 gacaagagga ctgtccagga ggtttcgaat ttccttaaac tgcccccttc gtatttcatc
     3961 aaaagcatac tggtgatgaa cgacaaggtc ccggtgcttg cgct
//
LOCUS       DATKOU010000088        14882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5117295, whole genome shotgun sequence.
ACCESSION   DATKOU010000088 DATKOU010000000
VERSION     DATKOU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5117295"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VMU21_04030"
     CDS             complement(<1..648)
                     /locus_tag="VMU21_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76726.1"
                     /translation="MAARLNIISFAVKNLRRKIVRTVLLVLAVTVVTGTLFSATLFIS
                     SMQNALKIGTYRLGADVLVVPDKYLSEARAALLAGEPTNFLMDRSVLDRVRDVEGVKR
                     ASPQLFIKPTSFTCCFNVNAFLVAFDPKSDFTVTPWLEKNLKKPLVGNEIITGRGLPV
                     IAGDSIPFFGTLFKVVGTMEPTGMKFFDQSVFMTMDAAYQMTENSRTKSMQPIFIE"
     gene            complement(759..1856)
                     /locus_tag="VMU21_04035"
     CDS             complement(759..1856)
                     /locus_tag="VMU21_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HUO76727.1"
                     /translation="MKRSFVVISLLILVLAAAAYAVMEDTCMTGPSPSIEAKVVPAEK
                     PIMFDAIAYIAGHGGHVAVIDMRTMKPPTDMEKGRIVLTEAGGEMEGKIAGMSFEEVK
                     AGGGTHGQAIVSEKGKKVLVAGTLNGNVYKVDLATGKKTGPYKVGEKFCGAIVGPDGH
                     IYLEDMADGNVYIWDSKTLKTVDKLPVGKAVCGIQWTKNAKKAYVTDMPTGVVYVIDW
                     KTKKTIKEITSPEMTFVHQARMTPDGKYLFVSAPNEYDPGLKPPTHKSQIIVINTEKD
                     EIVKRIILPDDVRPHDFAFTKDGKYAFLSSRTYGDDSKVFVMDLKNANIVESVSACAA
                     CHKEAGVTVKMDKGSPLLCGIEVDWSPSKKM"
     gene            complement(2135..2293)
                     /locus_tag="VMU21_04040"
     CDS             complement(2135..2293)
                     /locus_tag="VMU21_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76728.1"
                     /translation="MIDLQGKLVEVIANDITYTGTLVEISETEVYLEGESGWIVIPVE
                     QVASISEK"
     gene            complement(2290..3099)
                     /locus_tag="VMU21_04045"
     CDS             complement(2290..3099)
                     /locus_tag="VMU21_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUO76729.1"
                     /translation="MKILVKSLVDFFRDGGLMLAGSLSYFSMMAFVPFCLFLVTIFGY
                     FLGEQKEFYQFFLSKLISFFPKVTSQVTEELKMIISHRGIGKFTLLLYGLLSYQLFSS
                     MESAINVIFRVKVKRSFFISLILSLFVVTLISAFFLVSFSASSSISMLEPLQELFPVL
                     KMSRITGIFIGFVIPFILMFLTVTTVYILLPKKSVRLAHACSGALFTSIFLEVAKHVF
                     TFYVRHVFRLGTIYGPLSAFVIFLLWVYYSSCIFLIGAKVVYNQQAPGERT"
     gene            complement(3113..4429)
                     /gene="der"
                     /locus_tag="VMU21_04050"
     CDS             complement(3113..4429)
                     /gene="der"
                     /locus_tag="VMU21_04050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HUO76730.1"
                     /translation="MPKPFVVIVGRPNVGKSTLFNRIAGGETAIVESIPGVTRDRNYQ
                     EAEWGGKTFVIVDTGGFYPEPAEDIFPQVKEQAEFAIEEADLIIHLLDGKEGLTPHDT
                     DLSRMLRASGKKVLWAVNKIDAPTREDRLYDFYSLGVDDLVPLSAATGYEFNEFMDRV
                     ASLLPKREQGGEDLDYPKIAVVGRPNVGKSTLVNTLLGKTRMIVSPRPGTTRDSIDSV
                     CTYYGKKYLVIDTAGIRKKGKVGYSIERYSMVRAIRSIERCDVTLVVLDASDGIVEQD
                     RRIAGIVGQYGKGAVFLFNKWDLVREPDIVYKRYMHEFRDKIWFFNHAPVLTLSGLER
                     KRVTKVFPLIDGVVSQRKKRIATAELNTLLRETISRSPIPLYKGKTVKFYYMTQVGIE
                     PPQFVLFANYPEGVKDSSLRHVEKILREQFSFSGTPLRIFMRKRTQ"
     gene            complement(4439..4915)
                     /locus_tag="VMU21_04055"
     CDS             complement(4439..4915)
                     /locus_tag="VMU21_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUO76731.1"
                     /translation="MVSPEERLKELGIELPGTPAPLGAYVPCVRSGNLLFLSGMLPLR
                     KGKLTRTGRVGEGVSLGDAQEDARQVVINALSIMKAELGTLEKVVRCVKVTGFIASAA
                     DFVQQPAVLNAASELLLTIFGDRGRHARAAVGVPVLPLNSPLEVEFIFEVTEKETA"
     gene            complement(4954..5133)
                     /locus_tag="VMU21_04060"
     CDS             complement(4954..5133)
                     /locus_tag="VMU21_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76732.1"
                     /translation="MSEEHEKLVKTTLHLEAEVMEALEELAKEYSQETGQKWSRGAVI
                     RVALSEFFSRRGKIL"
     gene            5195..7279
                     /gene="uvrB"
                     /locus_tag="VMU21_04065"
     CDS             5195..7279
                     /gene="uvrB"
                     /locus_tag="VMU21_04065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HUO76733.1"
                     /translation="MGISLEPIPLRVIDSHMDFMLSTDFIPRGDQPKAIKELTQGIEK
                     GSRHQVLLGVTGSGKTFTIANVIAHVKKPTLVIAHNKTLAAQLYGEFMELFPENAIEF
                     FVSYYDYYQPEAYIPTTDTFIEKDAMINDDIDRLRHSATRAVLERRDTIVVASVSCIY
                     GIGSPQDYREMHLFIEEGMRAERDEILKRLVEMQYNRSEGEFRRGVFRVRGDSVEVYP
                     SFSLNKAIRLEFFGDSIDSIHEFDPLTGERLRSFERFALFPNSHWITPRERMEPALKK
                     MEDEMKERVAFFIKEGKPLEAQRIEQRTRFDLEMLREFGYCHGIENYSRHLSGRSPGE
                     PPYTLIDYFPPDFLIVVDESHATIPQVGGMYEGDRSRKQTLIDFGFRLPSALDNRPFT
                     FGEFEHRVNQAIYVSATPGPYEIDKSKGRVVQQIIRPTGLIDPGMKVKPASGQVDDLF
                     SEIRKRVEKGDRVLVTTLTKKMAEDLTDYYIELGVRARYLHSDIDTLERVELLRDLRR
                     GKFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTAGRAARNVAGEVIL
                     YADTITGSMKRAVEETERRRKIQKKYNEEMGITPETVKSTIKDILGSIYESDYWTIPV
                     AEEISAYNYDEETLRRLDEEMKEAAKKLDFELAIEIRDKIKEIRKKLIERGIQTSAAP
                     GTKKRKKKAGRS"
     gene            complement(7353..7799)
                     /locus_tag="VMU21_04070"
     CDS             complement(7353..7799)
                     /locus_tag="VMU21_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUO76734.1"
                     /translation="MGQIALRWVGEDSKMFIGRDSYGHTVVAGSWPREGGEQWQEWKA
                     LKPSDLLVISLCSCSAYDVVEILKKQRQRLSGLDITADAKQAPEPPYHFTDIHLHYVI
                     TGQDIAARKVEKAIELSQNKYCSVAATIRGVAKITYSFEVKGESAG"
     gene            7996..9255
                     /locus_tag="VMU21_04075"
     CDS             7996..9255
                     /locus_tag="VMU21_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HUO76735.1"
                     /translation="MSSKKGMIALMGSGELTATMVEVHKEILTHLGNAPKVCFLDTPA
                     GFQLNADQLSQKAVEYFRVNIGHPMEVVSFKSRDVPALEAEEAFRILKEVDYLLVGPG
                     SPTYAIRQWKGTPIPEILKRRIERGACLVAASAAAITVGRFSLPVYEIYKVGQELHWV
                     GGLDILGHFGLRVVVVPHWNNAEGGTHDTRFCFMGENRFRMLESLLPEETGILGIDEH
                     TACIIDLDREEALIKGIGTVTLRHQEFEKVFVRGERFPIRMLSEKGAGAVRTRRPSEM
                     PIPERAPVPEDRGFWNAVHAVEAMLSNGLAHDVRNATNALLELDRLIWQAQQESESPE
                     FISQARELMREFIVLLGVRLASEPKSPLECLAPLVEELLALRALFRENNQWREADAIR
                     DCLKRVHIEVDDTKEGYLWRIASEPRK"
     gene            9299..10324
                     /locus_tag="VMU21_04080"
     CDS             9299..10324
                     /locus_tag="VMU21_04080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO76736.1"
                     /translation="MKNVKVMRDRAEDVASAGTVTEEPLHNLEHYWQDTENPLRWDCI
                     FMLPPWLGAWWQYFGHGLKTRLCVVRHYGTVLGLVPLTFGGDSVRLISDSDLIDYSDF
                     IIAPLREREFFPAIVDYLRREGIRRLTMDRVRADSVVVSYFRDHSSLLGCEFSCESVD
                     MLYEMDLPDSWEGYLALLPGRERHETRRKLARLESTAHVGLQVIEDTKDVSAEMDTFM
                     ALFRSNRREKARFMSDTMESFFRSLAMEMAEAGLLKLFLLDLNNTPVAATFCFDHHST
                     VYLYNNGYDRHFSNLSVGLLSKVFSIRESIIRGRRSYNFLRGNETYKRRLGGSPLKLL
                     RCEVILK"
     gene            10321..11625
                     /locus_tag="VMU21_04085"
     CDS             10321..11625
                     /locus_tag="VMU21_04085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76737.1"
                     /translation="MTSASVIAPHHTTDGYRIRSGTRPLRIAMLSIHSSPLGELGTGD
                     TGGMSVYIREISRELGRAGHRVDIYTCAGSGKLNGEELLSENVRLIHLRIGNNGHIGK
                     SMLFGYLPEVFLSIEAHMTANNICYDLIHSHYWLSGSVGRLAQDRWYVPHIMMFHTTG
                     IAKRMACGQEREPVFRLITEKRLAHASDRVLAATENEKNLLMKYYDVSGKNIGVVPCG
                     VNLQQFHPVARGLARKELGLHDSQFIALYVGRFAPVKGLERFLAAAAHLRFHRGLRFV
                     IIGGDDKKTSASTELRRLAKRASLGDVVQFRGRIEHDLLPLYYSAADVLVVPSYYESF
                     GLVALESLACGTPVIATRVGAMDTLIQDGRTGFLVDSPGPLSLASAIENIMIAPLNGG
                     MSRDQIRASVLGYGWPDIASAIAREYTTVFQPVAYKKAITRE"
     gene            11639..12334
                     /locus_tag="VMU21_04090"
     CDS             11639..12334
                     /locus_tag="VMU21_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HUO76738.1"
                     /translation="MGVSADILVVTAHPDDAEFGVAGTVARWIGEGKQVVYAVCTNGE
                     KGTTDRTIDPRALAATRKREQEAAARLLGVKDVVFFGLPDQELEDTAEFRKMVVRAIR
                     TYRPATVVTSDPYHRYIWHRDHRIVGQVTLDAVFPFARDHLAYPDLLEEGLEPHKVRE
                     MLFFGSEDVNYRSDITATFHLKVAALRCHESQIKELKIRDLEAWMREWSCSMAEGAEC
                     QLAEAFHRIEITR"
     gene            complement(12380..12700)
                     /locus_tag="VMU21_04095"
     CDS             complement(12380..12700)
                     /locus_tag="VMU21_04095"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO76739.1"
                     /translation="MNDQHGEERRRSQRFPFREDILIDGAKLCTSNEISEGGLFVSAI
                     QIFEEGEVIDVTIPLGGEEITVKGQIKYCQPGIGMGVMFHNLTGEQKVKIKKLVDRVS
                     GRSS"
     gene            13216..13830
                     /locus_tag="VMU21_04100"
     CDS             13216..13830
                     /locus_tag="VMU21_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUO76740.1"
                     /translation="MIKDIHWLGHDTFKIVGEKVIFTDPFKLKKKDMADIILITHEHY
                     DHCSPDDVKKIQGPDTMIVAPADCAKQLSGNIKVIKVGDSINVAGIEVEAVPSYNTNK
                     QFHTKDKGWVGYIFTVKGQRIYIAGDTDYIPEMKQFKADIALLPVSGTYVMTAEEAIH
                     AALDIKPKIAIPMHYGSIVGGEGDAKRFAEGLKGKIEVAILHAE"
     gene            14074..14847
                     /locus_tag="VMU21_04105"
     CDS             14074..14847
                     /locus_tag="VMU21_04105"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HUO76741.1"
                     /translation="MDRLRELIKGEIGRDVVFLGTLESTNSFAMELADKGACHGTVVI
                     ADSQTKGRGRMGRIWISPSGGNIYMSVILTPPIKLQEATVLTIMAGVACCGALRNITG
                     LPVEIKWPNDLMVSHKKLGGILTEIKSGASKMVYAVMGIGINVNTKLEDFPSEVRTIA
                     TSIRNEKGKTGSRTFLIAEILNELDRWYRVFAQEGKKNLLTEWRRLTSMMGKPVQVTM
                     GEEIINGLAHDIDDEGRLLVRLPSGALRRISFGDVVILR"
BASE COUNT         3752 a         3593 c         3871 g         3666 t
ORIGIN      
        1 ttctatgaag atgggctgca tggatttagt gcgtgagttc tcagtcattt gatacgctgc
       61 atccatggtc ataaaaacgg attgatcaaa aaacttcatt cctgtgggtt ccatagtgcc
      121 cacaactttg aagagagtac cgaagaaggg aatgctgtcc ccggcgatca ccgggagtcc
      181 cctccctgtg attatttcat tgcccaccag gggcttttta aggttttttt cgagccaggg
      241 ggtgacagta aaatctgact tgggatcaaa ggcaacgagg aaggcattca cattaaagca
      301 gcaggtaaag gacgtcggct tgataaaaag ctgtggagat gccctcttta ctccttcaac
      361 atctctcacc cggtcgagaa cagaacgatc cattaagaaa ttcgtaggct ctcctgcaag
      421 caatgcggca cgggcttcag acaaatattt gtcagggaca accaggacat ccgcgccaag
      481 cctgtaggtc ccgattttaa gggcattctg catgctggag ataaaaaggg tcgcggagaa
      541 cagggtaccg gttacgacgg ttacggcaag gacgagcagc acggtcctta cgatcttccg
      601 tcttaagttt tttactgcaa aggagattat atttagtcgt gcagccatgt taaacgatcc
      661 tcatatatct actatagcat taagaaagaa ggggggcaaa tcgtgtagcc ccccttctga
      721 cagcctgagc tgtgtttacg gaatgaacgg tgagcaggtc acatcttctt gcttgggctc
      781 cagtcaacct cgataccgca gaggagcggt gaacccttat ccattttgac tgttacgccg
      841 gcttccttat ggcatgcagc gcatgcagat acgctctcca cgatattggc attcttcagg
      901 tccatgacaa aaaccttgct gtcatcaccg tatgtccttg atgagaggaa cgcatacttg
      961 ccgtccttgg taaacgcgaa atcgtgaggt ctcacatcat cgggcaggat gatacgtttc
     1021 acgatctcat ccttttctgt gttgatcacg atgatctggc tcttgtgcgt gggcggcttc
     1081 agtcccggat cgtattcatt gggagcgctc acaaagaggt acttgccatc aggggtcatc
     1141 cgtgcctgat ggacgaatgt catctcaggg ctggtgatct cctttatggt cttcttggtc
     1201 ttccagtcta tgacatatac tacgcctgta ggcatgtcgg tgacgtacgc cttcttggca
     1261 ttcttggtcc actggatgcc gcacactgcc ttgcccacag gcagcttgtc cacggtcttc
     1321 agcgtcttcg aatcccatat gtagacattg ccgtctgcca tgtcctcgag atagatatgg
     1381 ccgtcagggc cgacgatggc gccgcagaac ttctcgccaa ccttgtaggg acctgtcttt
     1441 ttccctgtgg cgaggtcaac cttatagacg tttccgttca gggtgccggc aacaagcacc
     1501 tttttgccct tctccgatac tatggcctgg ccatgggttc ccccgcccgc cttcacctct
     1561 tcgaagctca tacctgcgat cttgccctcc atctcgccgc ccgcctctgt aagtactatc
     1621 ctgccttttt ccatgtccgt agggggcttc atagtcctca tatcaatgac cgcgacatga
     1681 ccaccatgtc ccgcaatgta cgcgatggca tcaaacataa taggtttttc tgcaggtact
     1741 acctttgcct ctatgcttgg actcggccct gtcatgcagg tatcctccat gaccgcatag
     1801 gcggcagccg ccaataccaa tatcagtaaa gaaataacaa cgaaacttct cttcatacat
     1861 tctccccttt cttgtctttt ttttgttaaa agctgtagtg cccatgtgac aaccgcacac
     1921 tgccaatctc ctgctctgag atcacctcct ctttttgtgg catgatttcg cacatcctta
     1981 attggggatt taagagaccg gcaaaaacct gaagatacaa ggtcatagtg ttttatagtg
     2041 taatagaatt ccgcttcttg tcaacttcaa attgtaagtt tagaacaagg aataatagtg
     2101 gttatccata aaataggatt ctctccttgt gagctcactt ctcgctgatg gaggcaacct
     2161 gctcaactgg gatcacgatc cagccagatt cgccctcgag atagacttcc gtttcactta
     2221 tctccacgag ggtacctgta taggttatgt catttgcgat gacttcgacc aatttccctt
     2281 gcaaatcaat catgttcttt cccctggagc ttgctgatta tataccacct tggcgcctat
     2341 caggaatatg catgaagaat aatagaccca taacaagaat atcacaaaag ccgacagagg
     2401 gccatagatt gtgccgagcc tgaagacgtg gcgcacataa aaggtgaaga catgctttgc
     2461 cacttcaagg aagatggagg tgaacagggc tccggagcat gcgtgggcaa gcctcacact
     2521 cttttttgga agaagaatat agacagtagt gacagtcagg aacataagga taaaggggat
     2581 gacaaaccct ataaagatgc cggtgatcct gctcatcttc aggaccggga atagttcctg
     2641 cagaggttca agcatcgata tgcttgaact ggcactgaag gagacgagga aaaaggcgct
     2701 gataagcgtc accacaaaga gggagagtat cagggagatg aagaaagagc gcttgacctt
     2761 taccctgaag atcacgttga tggcggactc catggacgaa aaaagttgat acgaaagaag
     2821 accgtaaagg agcagggtga actttccgat gcccctatgg gagatgatca ttttcaattc
     2881 ttcggtgacc tgggaggtga ccttcgggaa aaagcttatg agttttgaca aaaagaactg
     2941 gtagaactcc ttttgctcac cgaggaaata cccgaagatg gtcaccagaa aaagacaaaa
     3001 agggacaaag gccatcatgg aaaagtaaga gagggacccc gcgagcatca gaccgccgtc
     3061 tctgaaaaag tccacgaggc ttttcaccag tatcttcatg tatccctccc attcactggg
     3121 ttctttttct cataaaaatc ctcaaaggcg ttccactgaa agagaactgc tccctgagta
     3181 ttttctccac atggcgcagt gaagaatcct tcaccccttc tgggtaattg gcaaagagta
     3241 cgaactgcgg aggctctatc cccacctggg tcatataata gaactttact gtcttccctt
     3301 tgtatagggg gatcggtgag cgggatatgg tctctctcag gagtgtgttg agttcagcag
     3361 tagcgatacg tttttttctt tgggagacca ctccatcgat gaggggaaaa accttggtga
     3421 cccttttgcg ttccaggccg gagagggtga ggacaggggc atggttgaag aaccatatct
     3481 tgtccctgaa ttcatgcatg taccttttgt atacgatatc gggctccctt accaggtccc
     3541 atttgttgaa taggaacacc gcgccttttc catactgccc cactataccc gcaatccgcc
     3601 tgtcctgctc cacaatgcca tcagacgcat caaggaccac aagggtcaca tcgcaacgtt
     3661 ctatgctcct gattgcccgc accatggaat agcgttcaat ggaatacccg acctttccct
     3721 tcttccttat acccgcggta tcgatgacga gatatttttt cccgtagtac gtgcatactg
     3781 aatcaatgga atcacgggtg gttccgggac gggggcttac gatcatacgt gtttttccaa
     3841 ggagggtatt taccagcgtg gatttcccca cattgggtct tcccacgaca gcgatctttg
     3901 ggtaatccag atcttccccg ccctgttctc ttttgggaag aagggaggct accctgtcca
     3961 tgaattcatt gaattcatag ccggtcgcag cagaaagagg gaccagatcg tccacaccga
     4021 gagagtaaaa atcatacagt cggtcttccc ttgtaggggc atcaatctta tttactgccc
     4081 agaggacctt cttccccgaa gccctgagca ttcttgaaag gtcagtgtcg tggggggtca
     4141 gcccttcttt cccgtccaga aggtgaatga tgaggtcagc ctcctcaatg gcaaactctg
     4201 cctgttcctt cacctggggg aaaatatctt ctgcaggttc aggataaaaa ccccctgtat
     4261 ccacaatgac aaaagtcttg ccgccccatt ccgcttcctg gtagtttctg tccctggtga
     4321 caccgggaat ggattccacg atggcggttt cgccgcctgc tatcctgtta aagagggtgg
     4381 atttccctac attcggcctg ccgactatga ccacgaaagg ttttggcatt gtttgggatc
     4441 aggcagtctc tttctcagtg acttcgaaaa tgaattccac ttccaatggt gaattgaggg
     4501 ggagaaccgg gaccccgaca gcggccctgg cgtgccttcc gcggtcaccg aagatggtga
     4561 ggagaagttc ggaggcagca tttaagaccg ctggctgctg cacgaaatca gcggctgatg
     4621 caataaagcc ggttaccttc acacatctga cgaccttctc caaggtaccg agctctgcct
     4681 tcataatgct cagggcattg atcaccacct gccttgcgtc ttcctgtgcg tcgcccaggg
     4741 aaaccccttc acctactctt ccggtccgcg tgagtttgcc tttccggagg ggcaacatcc
     4801 cgctgaggaa cagaaggttc ccagagcgga cacaggggac atatgcacca aggggcgcag
     4861 gtgttccggg gagttcaatc cccagttctt tcagtctttc ttcgggtgaa accataggat
     4921 ttgctcctcc tcaagaagga attgattgga gggttaaagt atctttcctc tccgtgaaaa
     4981 gaactcgctc aatgctactc tgatgacggc ccccctggac catttctggc ctgtctcctg
     5041 cgaatattcc tttgcaagct cttccagggc ctccatgacc tcagcttcga ggtgtagcgt
     5101 ggtctttacc agtttttcgt gttcttcgct catgactcat cagtactgaa tatgcatttt
     5161 gtatggattc taccagaaaa tcgcagaatt gaaaatgggc attagtcttg aaccaattcc
     5221 tctgagggtg atagactcac atatggactt catgctttcc actgatttca ttccaagagg
     5281 tgaccagccg aaggcgatta aagaactcac gcaaggaatt gaaaagggtt caagacatca
     5341 ggtgcttttg ggggtcaccg gctctggcaa gaccttcact attgccaatg taatcgccca
     5401 tgtgaaaaag ccaacccttg tgattgcgca caataagacc ctcgcagccc agttatacgg
     5461 agaatttatg gaactgtttc ctgaaaatgc cattgagttc tttgtgagct attacgacta
     5521 ttatcagcct gaggcataca tccccaccac cgataccttt attgaaaagg atgccatgat
     5581 caacgatgat atcgatcgtc tcaggcactc ggccacaagg gctgtccttg agcggaggga
     5641 taccattgtg gtcgcctctg tctcctgcat ttatggcata ggttcccccc aggattatag
     5701 ggagatgcat ctcttcatcg aggaggggat gcgtgccgag agggatgaga tactgaaaag
     5761 gcttgtggag atgcagtata accgctctga gggtgaattc aggaggggcg tcttcagggt
     5821 gaggggagat agcgtggagg tgtatccctc tttttctctg aacaaagcga tacggcttga
     5881 gttttttggt gactccatag attcaatcca tgagtttgac cccctgaccg gtgagagact
     5941 gcgaagcttt gagcggtttg ccctcttccc taacagtcat tggattaccc cccgggagcg
     6001 tatggagcct gcgctgaaaa aaatggaaga cgagatgaag gagcgggtag cgtttttcat
     6061 aaaagaaggg aagcccttgg aagcacagag aatcgagcag aggacgaggt ttgatctgga
     6121 gatgcttagg gagtttggat actgccacgg catcgagaac tattcaaggc acctcagcgg
     6181 caggtccccc ggtgagcccc cctacaccct catcgattac ttccccccgg actttctcat
     6241 cgtggtggat gaatctcacg caaccatacc tcaggtcggc ggcatgtacg aaggggatag
     6301 atcaaggaag cagaccctca tagatttcgg gttcaggctt ccctctgcac tggataacag
     6361 gcctttcacc ttcggggagt ttgaacacag ggtgaatcag gccatctatg tctccgcaac
     6421 accgggtccc tatgagattg ataaatccaa ggggagggta gtgcaacaga tcatcaggcc
     6481 cacaggatta atcgatcccg gcatgaaggt gaaacccgca tcaggacagg tggatgacct
     6541 cttcagcgag ataagaaaaa gggttgagaa gggcgacagg gttctcgtga ccacgcttac
     6601 caagaagatg gctgaggacc tcaccgatta ttatattgag cttggggtga gggcgcgtta
     6661 tctccattca gacatcgata ccctggaaag ggtggagttg ttgagagacc ttcggcgggg
     6721 gaagttcgac gtgcttgtag gggtgaatct cctaagagag ggtctcgacc ttcccgaagt
     6781 atcccttgtg gcgatccttg acgctgataa ggagggcttt ctcaggtcag agcgctccct
     6841 gatccagaca gcgggcaggg ctgcgcggaa tgtggccgga gaggtgatcc tctatgctga
     6901 caccatcaca ggttcgatga agagggcggt ggaagagacc gagaggagga ggaagatcca
     6961 gaaaaagtat aacgaagaaa tgggcatcac gccggagacg gtgaaatcca ctattaagga
     7021 tatcctgggt tccatctatg aatcggacta ctggactata ccagtcgcag aagaaatttc
     7081 tgcatataat tacgatgagg aaaccttaag aaggctggat gaagagatga aggaagcggc
     7141 aaaaaaactg gacttcgagt tggccatcga gataagggac aaaataaaag aaatcaggaa
     7201 aaaactcata gagagaggga tacagacatc agccgctccc gggaccaaaa agagaaagaa
     7261 aaaggctggc agaagttagg caggcacgga caattcctca cctgttcttt gcagtaacac
     7321 aagctggtca gtctatttgg gaactgcaaa ttttagcccg ctgattcgcc tttcacttca
     7381 aaactgtacg taatctttgc tacgcctcga attgtcgccg ccacagaaca gtatttgttc
     7441 tgcgagagtt caatggcctt ctccactttt cttgcagcga tgtcctgtcc ggtaattacg
     7501 taatgcaggt ggatgtcggt gaaatggtac ggcggttcag gggcctgttt ggcatctgca
     7561 gtgatgtcca ggccgcttaa cctctggcgc tgttttttga ggatctccac cacgtcatac
     7621 gccgagcatg agcaaaggct gatgacaagc aggtccgagg gtttgagagc cttccactcc
     7681 tgccattgct caccgccttc ccttggccag gaccccgcca ccaccgtgtg gccgtagctg
     7741 tcccgtccaa taaacatctt actgtcttca ccgacccatc gcagggcaat ttgtcccata
     7801 tgacttccct cctctttttc atagcttatc atagccctct gtgtatttcc cgttctctac
     7861 cctgagataa cagccatcat ttcctttgtg cagtatcaga gacttgacaa cacacagaaa
     7921 acacgctcaa attaagatat atgtgaaacc cgggaaagca aaagaaaagg aagaaagaac
     7981 gtaggaggat agttcgtgtc ttcaaagaaa ggaatgatcg cccttatggg ctcgggtgaa
     8041 ctcactgcca ccatggtgga ggttcacaag gagatcctta cccatcttgg gaacgctccc
     8101 aaggtgtgct ttctcgatac gcctgccggg tttcagttga atgccgatca gttgtcccag
     8161 aaggcggtag agtatttccg ggtcaatatc ggtcatccga tggaagtggt atcgttcaag
     8221 tccagggacg tcccagccct ggaggctgag gaggcattcc gcatactgaa agaggtcgat
     8281 tatcttctcg tcggtcccgg gagccccacg tatgccatac gtcaatggaa gggaaccccc
     8341 atccccgaaa tactgaagcg gcggatcgaa aggggtgcgt gtcttgtcgc ggcaagtgca
     8401 gcggcaatca cggtgggacg cttttccctg cctgtatacg agatctataa ggtcggtcag
     8461 gagttgcact gggtaggggg ccttgacatc ctgggccatt tcggtctgag ggttgtggta
     8521 gttccgcact ggaacaatgc tgagggcggg acccatgaca cgcgcttctg ttttatggga
     8581 gaaaaccgtt tcaggatgct tgagtctctg cttcctgagg agacaggtat cctcggcatt
     8641 gacgaacaca ctgcctgcat catagacctg gacagggaag aggccttgat aaagggtatt
     8701 ggcacagtga cgctgcgcca ccaggaattt gagaaggtat ttgtgagggg agaacggttc
     8761 ccgatcagga tgttatcaga gaaaggggct ggtgctgtcc ggacaaggag gccttcggag
     8821 atgcccatcc cggagcgcgc acccgttcct gaggacagag gcttctggaa tgcggtacat
     8881 gccgttgaag ccatgctcag caatggtctt gcccatgatg tccggaacgc gaccaatgct
     8941 cttctggaac tagacaggct tatctggcag gcgcagcagg aaagtgaaag cccggagttt
     9001 atttctcagg caagggaact tatgcgcgaa ttcatcgttc ttctcggcgt acgtctcgct
     9061 tctgagccaa agagcccgct cgagtgtctc gctccccttg tggaggaact gcttgcatta
     9121 agggcccttt tcagggaaaa taaccaatgg cgtgaagcag atgcgattcg tgactgtctc
     9181 aagcgggtac atatcgaagt agatgacacc aaggaaggat acctctggcg tatagcatct
     9241 gagccgcgaa aataggatcg aagaaaatat tctcctgaaa ggaacggata gttatcgcat
     9301 gaaaaacgtt aaggtcatga gggacagggc tgaagatgtc gcctctgccg ggacagtgac
     9361 tgaagagccg ctgcataacc tggaacacta ctggcaggac accgaaaacc cactgagatg
     9421 ggattgcata tttatgctcc ctccatggct cggtgcctgg tggcagtatt tcggacatgg
     9481 cctgaagaca cgtctctgtg tggtgaggca ttatgggact gttctcggcc ttgttccgct
     9541 cacgtttggt ggggattcgg tccggctcat cagcgacagc gacctcatcg attacagcga
     9601 ctttatcatt gcacccttaa gggaaaggga gtttttccca gcgattgttg attacctgag
     9661 gcgtgagggc attcgccgtc tcaccatgga ccgcgtccgt gctgattccg tggtggtatc
     9721 gtatttccgg gatcactcat cgctcctcgg ctgcgagttc tcatgtgagt ctgtggatat
     9781 gctctatgaa atggatctgc cggactcgtg ggaagggtat cttgctctcc tgccgggcag
     9841 ggagaggcat gagacgcgcc ggaagctggc gcgtttggaa agtactgctc acgtagggct
     9901 gcaggttatc gaggacacga aagatgtctc ggcagagatg gacaccttta tggcactttt
     9961 tcggtctaat cggagagaaa aggcccgttt catgagcgat accatggagt cttttttcag
    10021 atctctcgcg atggaaatgg ccgaggcggg actattaaag ctgttccttc tggacctgaa
    10081 caacacaccc gttgctgcga ccttctgttt cgatcatcac tccacagtct atctttataa
    10141 caatggctat gaccggcact ttagcaacct cagcgtggga ctcctgagta aggtcttcag
    10201 cataagggaa agtataatcc gtggcagaag gagctacaat tttcttaggg gcaacgagac
    10261 atacaaacgc cgtcttggcg gcagcccgct caagctcctt cggtgcgagg tgattctgaa
    10321 atgacatcag cctctgtcat tgcaccgcac cacacgacgg acggataccg catacgttcc
    10381 ggtacgcgcc ccctaaggat tgctatgctc agcattcatt caagcccttt gggtgaactc
    10441 gggacagggg acacaggggg tatgagcgtc tatatccgcg agatctccag agaactggga
    10501 agggcaggac accgtgtcga catttatacc tgcgcaggga gcggaaaact taatggtgag
    10561 gaacttctat ctgagaatgt gcgtctcatc caccttcgca tcggaaataa tggtcacatc
    10621 gggaaaagta tgctctttgg atatctccct gaagtgttct tgtccataga ggcgcatatg
    10681 actgccaaca acatttgcta tgatctcatc cacagccact actggctatc tggcagcgta
    10741 ggtcgcctcg cacaagaccg atggtatgtg ccccatatca tgatgtttca cacaacgggt
    10801 attgcaaaac ggatggcttg cgggcaggag agggagccgg ttttccgcct aatcactgaa
    10861 aagaggctcg cccatgcaag tgacagggtt ctcgcagcaa cggagaacga aaaaaatcta
    10921 ctcatgaaat actatgatgt atcagggaaa aatatcggag tggtaccctg cggcgtcaac
    10981 ctccaacagt tccacccggt tgcgagggga ctggccagaa aagagttggg gcttcatgac
    11041 tcccagttca ttgcccttta tgtaggcagg tttgcccctg tcaaggggct ggaaagattc
    11101 cttgctgcgg ccgcacatct ccgcttccac cggggactcc ggttcgtaat cattggcggt
    11161 gacgacaaga agacatctgc gtcgactgaa ctgcgtcgcc tcgctaaacg ggcctcttta
    11221 ggggacgttg tgcaatttcg aggcaggatt gagcacgatc ttctcccctt atactacagt
    11281 gctgccgatg ttcttgtcgt accgtcatac tacgagagtt tcggtcttgt ggcgctggaa
    11341 tccctcgcat gcggtacccc cgttatcgcg acacgcgtgg gcgccatgga tacattgata
    11401 caggacggca ggacaggatt tcttgttgac agccccggtc ccctttctct tgcatctgcc
    11461 atcgagaaca taatgatcgc tccgttgaac gggggaatgt cccgtgacca gattcgcgca
    11521 tccgttctcg gctatggctg gccggatatt gcatcggcaa ttgcgcggga atataccacc
    11581 gtctttcagc cggtagcgta taaaaaagcg atcaccaggg aatgaaagga gttcgcgtat
    11641 gggagtctct gctgatatac tcgtcgttac ggcacaccct gacgatgcag aattcggcgt
    11701 ggcaggaacg gtagcgcgct ggattgggga aggtaaacaa gttgtctatg cggtgtgtac
    11761 aaacggagaa aagggaacaa cagacaggac gatagacccc agggcattgg ccgcgacgag
    11821 gaaaagggag caagaggctg cagcaagact tcttggtgta aaagacgtgg tcttctttgg
    11881 gcttcctgac caggaattgg aagacactgc cgagttcaga aaaatggttg tccgggcaat
    11941 caggacgtat cgaccagcaa ccgttgtcac ctcagatcct taccaccgct atatctggca
    12001 tcgggaccac agaatagtgg gacaggttac cctcgacgca gtcttccctt ttgcgcggga
    12061 ccacctcgcg tatccggatc tgctggaaga aggactggaa cctcacaagg ttcgggagat
    12121 gctctttttt ggttctgaag acgtcaacta ccgctctgat ataaccgcga cctttcattt
    12181 gaaagttgcg gccctgaggt gtcacgaaag ccagataaag gaactcaaga tacgggacct
    12241 tgaggcgtgg atgcgggagt ggtcctgctc catggctgaa ggtgcggaat gccagcttgc
    12301 tgaggcgttc cacaggattg agatcacgag ataggttaga ttgccttaca gagacgaagg
    12361 atgggtggga gtgtggaact caggaactgc gacccgatac gcggtcgacc aatttcttaa
    12421 tctttacctt ctgttcgcca gtaaggttat gaaacataac gcccataccg atacccggtt
    12481 gacaatactt tatctgtccc tttaccgtta tctcttctcc accgagggga atggtaacgt
    12541 cgatcacctc accctcttca aaaatttgaa tcgccgaaac aaaaagacca ccttcgctta
    12601 tctcgttgct tgtacatagc tttgcgccat caatgagaat gtcttccctg aacgggaatc
    12661 gctggcttct tcttctttct tctccgtgct ggtcattcat aggcatcatg ctcctctcag
    12721 cttaacccat cagcttatca taacacagaa atattatgga ctggtccgaa tctgagatct
    12781 catatatagc attccagcaa atatcatgcc ttcaccaagt tctcacaata atccattgat
    12841 tttaatgaat gtaaagcaac ccgcgcgttt agccctgaca attgaaatgt cataagtgaa
    12901 cggttttcat aatgatgctt agtgcggaat gtccggtttt tgtacagcaa agttcgtaat
    12961 tcattagccc acacaataag agaataatca atggacaaga gtctttttat gagggacatc
    13021 tcttcagccc cagcaggtat tgtgctatat tatgcgtata agaaagccgg tagcgcgggt
    13081 taaaggtaga ctgcgattgc agataaaggg aaagagaagg attttgagtg agatgtgaag
    13141 ggctattttg ccgtatgcct gatatttttt gtgatgcctg cagtagtttt gtcaggcggg
    13201 aaggaggcaa aaacaatgat taaagacatt cactggcttg gacatgacac attcaagatt
    13261 gtgggagaga aggttatctt taccgatcca ttcaagctca agaaaaagga catggcggac
    13321 attatcctca ttacccatga acattatgac cattgttccc ctgatgacgt gaaaaagata
    13381 cagggacctg acaccatgat cgttgcccct gctgactgcg caaagcaatt gtcagggaac
    13441 attaaagtca tcaaagtcgg agatagtatc aatgtggctg gaatagaggt ggaggcagtt
    13501 ccctcctaca ataccaacaa gcagtttcat accaaggaca agggatgggt aggatacata
    13561 tttaccgtaa aagggcaaag gatttacatc gcaggggata cagactacat ccctgaaatg
    13621 aagcagttta aggcggatat cgctctcctc ccggtctcgg gcacctatgt catgaccgca
    13681 gaggaggcga ttcatgcggc cctggacata aagccgaaaa tcgccattcc catgcactac
    13741 ggatccattg taggcggtga gggcgatgcg aagagatttg cggagggatt gaagggaaag
    13801 atcgaggtag caattcttca tgcggaataa caataatcct gggtgtttct ggcccgctgt
    13861 attgggaagg ctgtcataaa atttctcata atcaacagat tttcttatgg cgttgatagg
    13921 ttcactagaa aatggatgtc ttgcgtcttt tcctcaacgg gtcttattgt gaggtgtcgt
    13981 attcgcctca gcgaatccgc aaaagaggat aagaattctt catcattctt tcgtatatag
    14041 ttttttctca acgctgtcaa caaaggatct cctatggacc ggctaagaga actcataaag
    14101 ggagagattg gaagggatgt tgtgtttctt ggaacgctgg agtccaccaa ttcatttgcc
    14161 atggaacttg cggacaaggg tgcttgccat ggaacagttg tcattgctga tagccagaca
    14221 aagggcaggg gcaggatggg aaggatatgg atatctccct caggtggtaa tatctatatg
    14281 agcgtaattc tcaccccacc cataaaactc caggaagcga cggtcctgac cataatggct
    14341 ggcgttgcct gctgtggtgc actgaggaat atcacaggcc tgccagtgga gattaaatgg
    14401 cctaatgacc tcatggtatc tcataagaaa ttgggtggca ttctcactga aatcaagtca
    14461 ggtgcatcga aaatggtcta tgcagtaatg ggaatcggaa taaatgtaaa cacaaaatta
    14521 gaggactttc cttccgaagt gaggacaatc gcgacatcca tcaggaatga aaagggaaag
    14581 acggggtcaa ggactttcct tatagctgaa atactgaatg agctggatcg ctggtatcgt
    14641 gtctttgcac aagaggggaa aaaaaacctc ctgactgaat ggcgaaggct cacctcaatg
    14701 atgggaaagc ctgttcaggt aaccatgggt gaggaaatca tcaacgggct tgcgcatgac
    14761 attgatgacg aaggtaggct gcttgtaagg ttgccatcag gggctttgag gaggatcagt
    14821 tttggtgatg tcgttatcct gcgatgaagc ggtaatcggg taacttatcc ggttggtaac
    14881 aa
//
LOCUS       DATKOU010000089         9884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_118042, whole genome shotgun sequence.
ACCESSION   DATKOU010000089 DATKOU010000000
VERSION     DATKOU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118042"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..968
                     /locus_tag="VMU21_04110"
     CDS             240..968
                     /locus_tag="VMU21_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUO76742.1"
                     /translation="MDGWKNNISFILIDTMETGNIGASARAMKNLGFSRLELVRPREF
                     PSDKAGWFAHGAQDILSTVQVHSGLVPALKDVSVVIGTTRRTGKKRGLVYPVREVVER
                     VRNLSEKNRVALLFGREDRGLTSDENSVCSFMIRIPAAPENPSFNLGQAVLIVAYELS
                     YTDYTCSQSPAIISNKDLSNLFQRLRKLMKMLGYAPKGIRDDEEKIMRDLKRLLAKAA
                     VTEREARMLHGILSQIEEASERKT"
     gene            complement(983..1639)
                     /locus_tag="VMU21_04115"
     CDS             complement(983..1639)
                     /locus_tag="VMU21_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HUO76743.1"
                     /translation="MDMILDDMEVRILGCLMEKEMTTPEYYPLSLNALINACNQKSNR
                     NPVVSFEESAVAHGLQLLQEKGLARKTHSAGSRVPKYLHAILDQFDLSRREMAVLCEL
                     LLRGPQTVGELRPHVERMSVFESLGEAEKSMQVLVEHNPPLAIRLPREPGRKECRYMH
                     LFSGEVGTEKAGYELPDEKSAPQKGALEKIIRLELEVARLRRELEELKQAFLLFKSQF
                     "
     gene            complement(1792..3135)
                     /locus_tag="VMU21_04120"
     CDS             complement(1792..3135)
                     /locus_tag="VMU21_04120"
                     /inference="COORDINATES: protein motif:HMM:NF010635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUO76744.1"
                     /translation="MDAILRKVQELALLHDKVDPRIVKEKNIKLGLRNEDGTGVFVGI
                     TSKGQVIGYEKVGHGDGIEQVNPVPGKLFYCGYDVVELVQEKENEGRFGFEETVYLLL
                     TGELPAKQDLKAFSQLLGKKRALPIEVKQMILNRPRHNDQMGSLHTIVSAMHLFDKHP
                     NSIDMNDVTRQCIELIAKFPTIIAYNYLTSTMEKKALSKLKEPDPQLSMAENFLHLLL
                     GEVPGRATAELFDTMLIFHAEHGGGNNSTFSTRCVSSSGANTYMALCAGIGSLSGHLH
                     GGANEAVVTMIQDIKQKVKNWESDDEVTAYLELIVDKKAGDRSGKIYGLGHAVYTVSD
                     PRAVFLEQRAGSLSKAVGRDEEFSLYKRIARIAPRLVKERKGKVVCTNVDFYSGFVYQ
                     CMGIPKELFTPIFAMARVAGWSAHRIEEIVQGRIIRPSYVTSLKGKKTYIPLSSR"
     gene            complement(3220..3783)
                     /gene="greB"
                     /locus_tag="VMU21_04125"
     CDS             complement(3220..3783)
                     /gene="greB"
                     /locus_tag="VMU21_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HUO76745.1"
                     /translation="MKKLLPHTSRVSPYITPDGLRRLSEELSYLWKSKRPQVTQAVAE
                     AAAMGDRSENAEYIYGKKQLRQIDARIRFLSKRLSEVVVVDRIPDDTSRVFFGAWVEL
                     EDTRGNKSRYRIVGPDEFDPDKGYISIDSPMARALLRKTEGEEVTVSSPKGTDTFVVV
                     SIHYGGSRVHGAKEQGGKGVALRRRDR"
     gene            complement(3938..5860)
                     /locus_tag="VMU21_04130"
     CDS             complement(3938..5860)
                     /locus_tag="VMU21_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUO76746.1"
                     /translation="MLKKTNRDLVYLSIVLFLAFFLTAFTLVLNFGHTRSEFLTSYIR
                     FPLTEALINFNFLFLLGLLFVLYRHWRKERSTRRRNEGTLLSLQKAVETMQIGVTITD
                     RNGKIIYINPADAEMHGYRVEELTGKDARIFAPPHIWKAKSPESMLMRFRRETMNTRK
                     DGSTFPVQLMSDVVTDAEGEVFAVITTCEDITERKRNEETIKQLAFYDPLTGLPNRSL
                     FNDRLNQELANARRHKELLAVMFLDLDRFKVVNDTLGHAVGDLLLQEVAQRLKGIVRE
                     GDTVSRFGGDEFVMIFPDTIRLEDISVIAEKILRNLSNVYVLNGTDVHITASIGISVF
                     PHNGDEQEVLVKNADTTMYYAKEQGRNNFQFYSATINENALERLILQSHLRKANNNEF
                     IVHYQPLVDLRSGRISGAEALVRWQHPEYGLLSPVKFIPLAEETGLISSIGKHVFFTA
                     CAQNKTWQEAGFFPIRMAINISVHQFNQKDFIKMLKGVLDKVGLAPEYVELEITESIM
                     MQNLEHTASTLNELKALGMHCAIDDFGTGYSSLTYLKQLPIGKLKLDKSFVRSLTKDP
                     NDDAISKAVISMAHSMNLRVVAEGVETADQLEFLRAHHCDEAQGFLFGKPMTAKEFVQ
                     LLIAGGEQYAGILVPA"
     gene            6753..8099
                     /locus_tag="VMU21_04135"
     CDS             6753..8099
                     /locus_tag="VMU21_04135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUO76747.1"
                     /translation="MKPLSELIATHEDWLMHRVLYYAKDYGYTKYTSTLAEAWRISIK
                     GLSDTLISAIHMHGAPPELDPDERVGEDPIAAFGIQEARRHRARGISIAMFLGLMKYY
                     RQSYIDLVLGSSFGDIERELYILFVNRVFDRVEIGFCSEWSAQENDARVDELQAANRS
                     ITNEKNKYLTIFESIHEPIFLLDRGNQIDNLNHSAALLLTGMNIPGSRYYDENPNNQY
                     LPWLNDKLKELETHTDGESAFDIDLETLEGARWFHVRLKRMLDVSEKFWGTVVTLNDI
                     TKRKQMEEQLHLQLLTDELTGLYNRRGFLVMAEQHVKMARRLKKGIIIIAVDMDGLKT
                     INDKYGHQEGDAAIAETALILKQCFRESDIIGRMGGDEFAVLLLEGVDVPPEQTVIDR
                     LNAHLRTRNENKGRDYQLSFSTGIFRCAPECIKSLDELLARADALMYQQKQSKRTL"
     gene            complement(8353..9720)
                     /locus_tag="VMU21_04140"
     CDS             complement(8353..9720)
                     /locus_tag="VMU21_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HUO76748.1"
                     /translation="MTEKAWIDLTTGSTWENIWRMSWPMLLVMFLNFLVGLTDIYVAG
                     FLGPEIQAIVGFVSQLYFFIIIVANAISIATVALVSRAVGAGKFGDALSVAKQSLLFG
                     IACALALTGAGLLFHDKIVSLAGFSGNVRQIADDFFIIFAFALSPNYLVILSNAIFRA
                     GGEVRLSLLAMFCISVINIGLNFLLVFGLSSFHGFGYRGIAFSTAAAMSAGTIVCLIL
                     FRKSRWKNIFSGPWYVSADLVRKIVLLSWPAALIQISWNAGGIVLYNILSHLLEGGIT
                     AMAALTNGLRIEAIIYLPAFALSMAASVLTGQNLGAGASERAQKIGWKIAVSGVLFVS
                     IMALPIFIWAGDITSPVAKDWQVLAETTRYLRVTMLSEPFMALSVILGGCLQGAGDTK
                     GAMLVIVSALWIIRLPLAYLLAVVLGYGAFGVWLAMVISMCVQGIAMTIRFRKGRWKE
                     VRPWG"
BASE COUNT         2509 a         2622 c         2374 g         2379 t
ORIGIN      
        1 aatccgtttc acctgagaaa ggatatggaa gagaagctca aaaggatctt cccgctatgc
       61 tgcaaaaaga ttcgttgagt ctccgatcct tcggtaacat tttcctatga gtcaactgga
      121 tgcccttttc gctacgacac ctcactgttc cttaacgtca gcactgagag atggttccgt
      181 acctttttca accacgcgcg cattgcactc accctccttc ttcctttatc cttatggtga
      241 tggacggatg gaaaaacaac atctccttta tactcatcga caccatggag acagggaata
      301 taggcgcctc ggcacgggca atgaaaaatc tcgggttttc gagactcgaa ctcgtccgtc
      361 cgagggaatt cccttcagat aaagcaggat ggtttgccca tggggcccaa gatattctct
      421 ccactgttca ggtgcattcg ggactggtgc ctgcattgaa ggacgtgtcc gtagtcatcg
      481 gcaccacaag gaggaccggg aagaagcgcg gccttgtcta tcctgttcgg gaggtggtag
      541 aaagggtcag gaatctgtcc gaaaaaaacc gcgtcgccct gcttttcgga agggaagaca
      601 ggggacttac cagtgacgag aactcggtat gctcattcat gatcaggatc cctgccgccc
      661 ctgaaaaccc ctccttcaat ctcggccagg cagtgctcat cgtggcctat gagctctcgt
      721 atacagatta cacgtgcagc cagtctcctg caatcatcag caataaggat ctctccaacc
      781 tctttcaaag actgagaaag ctaatgaaaa tgctggggta tgcacccaag ggtatcaggg
      841 acgatgagga aaagatcatg cgggacctga agcgactcct ggcaaaggct gctgtcacgg
      901 agcgagaggc gcgcatgctc cacggtatcc tctcgcaaat agaagaggcc tcagaaagaa
      961 agacgtgaac aggagggaat acttagaact gtgatttaaa cagcaagaag gcctgcttga
     1021 gctcctccag ctctcttctg agccttgcca cctccaactc cagcctgatg attttctcaa
     1081 gtgccccctt ctggggagcc gacttttcat ccggtaactc ataccctgct ttttctgtgc
     1141 ctacctcccc ggaaaaaagg tgcatgtacc tgcactcctt cctgccgggt tcccttggta
     1201 accgtatcgc caggggagga ttatgctcta ccagcacctg catacttttt tcagcttccc
     1261 ccaagctttc aaagaccgac atcctttcca catggggcct gagttctccc acggtctggg
     1321 ggccacggag caagagttcg cagagaacgg ccatttcccg tctcgacaga tcaaactggt
     1381 cgaggatcgc atggagatat ttgggaacgc ggctccccgc agagtgagtt tttcgcgcaa
     1441 ggcctttttc ctggaggagc tgcaatccat gcgccacggc agactcttca aaggaaacaa
     1501 cagggttgcg gttcgatttc tggttgcaag cattgatcag cgcattcaga gacaaggggt
     1561 agtattcggg cgtagtcatt tccttctcca taaggcagcc gaggatgcga acctccatgt
     1621 catcaagaat catatccatt ccgaaacctc cgtagtctcc cctgcaggaa agggccgcac
     1681 tctccttcct tatggagaat aagaacagga taagtgcaca tgaggactta cgccaaaaga
     1741 aagaaaaacc ctgctccccc tcgcacagaa gaacggctta catccgtccc ctcagcggct
     1801 ggagagagga atgtaagtct tcttgccttt caacgacgta acataagaag gccttataat
     1861 ccggccctgg acgatctcct cgatcctgtg cgcggaccag ccggccacac gcgccatggc
     1921 aaagatcggc gtaaaaagct ctttggggat acccatgcac tgatagacga agcccgagta
     1981 gaagtccaca ttggtgcaga ccacctttcc cttcctttcc ttcacaagtc gtggcgctat
     2041 gcgcgcaatc ctcttgtaaa gcgagaattc ctcgtcccgt ccgacagcct ttgagagtga
     2101 ccctgccctt tgctccagga ataccgcccg ggggtctgat acggtataca ccgcgtggcc
     2161 cagcccataa atctttccgg acctgtcccc tgccttcttg tccacgatca gctcaaggta
     2221 tgcggtcacc tcgtcatcgc tctcccaatt cttcaccttt tgcttgatgt cctggatcat
     2281 tgtgacaacc gcctcgttgg cgcctccatg caggtggccc gagagtgacc cgattccggc
     2341 gcagagtgcc atataggtat ttgcacctga ggatgagaca catctggtcg agaacgtgga
     2401 gttattccct ccgccgtgct cggcatgaaa tatcagcatc gtgtcaaaga gttcggctgt
     2461 ggccctgcca ggcacttcgc ccagcagcag gtgcagaaag ttctcggcca tgctcagctg
     2521 cgggtccggc tctttcaatt ttgaaagagc ttttttctcc atcgtcgacg taagatagtt
     2581 atacgcgata atggtcggaa actttgcaat gagctcgata cattgccgcg tcacatcatt
     2641 catgtcaatg gaattcgggt gtttgtcaaa gaggtgcatc gcagacacaa tggtgtgaag
     2701 agaccccatc tggtcattat gacggggtct gttgagaatc atctgtttta cttcaatggg
     2761 aagcgcccgt tttttgccca gcagctggga aaatgccttc aggtcctgct ttgccggaag
     2821 ttcacctgtg agaagcagat agacggtctc ttcaaagccg aaacggccct cattttcctt
     2881 ttcctgaacc agttccacaa cgtcatatcc gcaatagaag agcttccccg gcacaggatt
     2941 gacctgctca atgccgtcgc catggccgac cttctcatag ccgattacct ggcccttgct
     3001 cgtgatgccc acaaacacgc cagtgccatc ttcgttcctc aggcccagtt tgatgttctt
     3061 ttctttgaca atccgtggat cgaccttgtc atgaagtaag gcaagctcct gaacttttct
     3121 gagtattgcg tccatttttt ataccctctt ccgataattt tcaacaatag tattattaaa
     3181 gtatatctta agggaacaat gcaacaccct cagcacttcc taacggtcac gcctcctcag
     3241 cgctactcct ttgcctcctt gctctttggc accatggacc ctagagccac cgtaatggat
     3301 agatactacc acaaaagtat ccgtcccctt agggctgctc actgtcacct cttcaccctc
     3361 tgtcttccgt aacaatgccc ttgccatagg tgaatcaatg cttatatacc ctttgtcagg
     3421 gtcaaactca tcaggtccca caatgcgata cctggacttg ttccccctgg tatcctcaag
     3481 ttcaacccat gcgccaaaaa atacccttga agtatcatca ggtatcctgt ccacgacgac
     3541 tacctcgctc aatctctttg aaaggaaccg tatacgcgca tcaatctggc gcaactgctt
     3601 cttgccgtag atatactctg cattctcaga gcggtccccc atggcagcag cttcagcaac
     3661 agcctgagtg acctggggcc gtttactctt ccacagataa gacagttcct cacttaagcg
     3721 cctcaggccg tcaggtgtga tataaggtga gacacgtgaa gtgtgcggca atagcttttt
     3781 catatccccc ctatgtgctc attcattata acggaatcag accaagcctt gtagaaagcc
     3841 gggcagcgag gagttccttt tggcaacgtg agggtgatta tgaaggatgg ccaaaaggaa
     3901 acaaactcat acagggaaga aacgcggata tcgctgttca ggcgggaacc aaaattccgg
     3961 cgtattgttc tccccctgca atgagcagtt gcacgaattc ctttgcagtc ataggcttgc
     4021 caaagagaaa gccctgtgct tcatcgcaat ggtgagcgcg caagaattca agttggtcag
     4081 cggtttccac tccctcggca acgacccgta aattcatgct gtgcgccatg gaaataaccg
     4141 ccttggaaat cgcatcatcg ttgggatcct tagtgaggga acgcacaaaa gacttatcca
     4201 gcttcagctt tcctatgggg agctgtttca aataggttaa cgaggaatac cccgtaccaa
     4261 aatcatcgat agcgcagtgc attccgaggg ccttcagctc attgagtgtg gacgcagtgt
     4321 gttccagatt ttgcatcata atactttcag ttatctccag ttccacgtac tcaggcgcaa
     4381 gccccacctt atccaacaca cctttcagca tcttgataaa gtctttttga ttgaattggt
     4441 gcaccgagat attgatggcc atacgaatgg ggaagaaccc tgcttcctgc caggttttgt
     4501 tttgagcgca ggcggtaaaa aatacatgct ttccgatgga cgaaatgagc cctgtttctt
     4561 ctgcgagagg aataaattta acagggctca gcaggccata ctcagggtgc tgccagcgca
     4621 caagtgcttc tgcaccggaa atcctgccgc tccttaagtc caccaggggc tggtagtgca
     4681 caatgaattc attgttgttt gctttcctga gatggctttg gaggatgagt ctctccaaag
     4741 cattctcatt gatcgtcgca ctgtagaatt gaaagttatt tcttccctgc tccttggcat
     4801 aatacattgt agtgtcggca ttcttcacaa ggacttcctg ttcgtcaccg ttgtgaggaa
     4861 atacgcttat gccgatgctt gccgtaatgt gaacgtcagt cccgttaagc acatatacgt
     4921 tggacagatt cctcaggatc ttttccgcaa taaccgagat gtcctcaagg cggatggtat
     4981 ccggaaagat catcacgaat tcatcgcctc cgaagcggct gaccgtatct ccttcacgta
     5041 cgatgccctt taaccgctgc gccacctcct ggagtaggag gtcacctacc gcgtgcccca
     5101 gggtgtcgtt caccacctta aaccggtcga ggtcaaggaa cattactgcg agcaactcct
     5161 tgtgcctgcg tgcatttgcc aattcctgat tcaaacggtc attaaataac gaacgattcg
     5221 gaagaccagt gagggggtcg tagaacgcca actgctttat ggtctcttca ttcctcttgc
     5281 gttcggtaat atcctcacac gtcgtgatga ccgcaaaaac ctcgccctcg gcatccgtga
     5341 ccacgtctga catgagctgg acgggaaagg tactgccgtc tttcctggta ttcatagttt
     5401 cacgccgaaa cctcatgagc attgactccg gggactttgc cttccagatg tggggaggag
     5461 caaagattct cgcgtccttg cccgtcaact cttccactcg gtagccgtgc atttccgcat
     5521 cagcaggatt gatgtaaata atcttcccgt tgcggtcagt gatcgttacg cctatctgca
     5581 tagtctcaac agccttctgc aaagaaagga gtgttccctc atttctcctg cgcgtgcttc
     5641 tttcctttct ccagtgacgg tacaacacaa acagaagtcc taataagaac aggaaattaa
     5701 aattaatgag cgcttctgtg agcggaaacc gtatgtatga agtgagaaac tccgaacggg
     5761 tatgcccaaa attcaagaca agcgtgaaag cagtgaggaa gaatgcaagg aaaagaacga
     5821 tggagagata aacgagatcc ctgtttgtct tttttagcat gttttatata tgttgagcat
     5881 tgtacactct actgagcacc attataccac gcaccccgtt tcttgttatg cagaatgttc
     5941 gccaccaaac agtgcagaaa tgaggtcagc tgaagaaacg gatctcacct cattgtgatg
     6001 cggtgtcgcc taccctatag ctccagttgc cagcggcgtg ctattctttc atcatccata
     6061 agacttcctg cacttcctcc ttctcttcct ttcctgccag ggatgtctcg acgcacaccc
     6121 ggatggaggt agaggacaat aatgaacatt accagctctc aagcaaagac catgccatag
     6181 actgccggat cgtttcctgt tgatgagttc gcacgaggcg cacagacatt aattgatccc
     6241 gggttccctt cctttccaag tgctcacccg atgaaaggat tcccatgtgt gtagaaatgg
     6301 cattctttaa tatgtttcat tggtgtccac tgtaccacgg cagcgttatc ctgagcaatt
     6361 tggatgtgtt ttctgttacc acacaagcca ccacaatggt tcctgggcgt aaaagccttc
     6421 agtcatctca gtaccatcgc gccatgcaaa gaattcattg tagcggtcat aaacttgacc
     6481 gatttccggt cgcaccgtcc gggttttgac cacagtgcgc catatgagac aattttctgc
     6541 tttgcgcctt tactcaccaa tgacaaaaaa acattccgta tcatagggtt ctcaggaata
     6601 gggcgtattt ctgctttatt tgtcagactg gcatcggtct tgccctatac acattattgg
     6661 aataggctga tgaaccttta ccgcgaagac agaaaacgtt cataattaag gagagtgagg
     6721 cactgcatat gcaagaggat gcacctttca acatgaaacc cctttcagaa cttattgcca
     6781 cccacgagga ctggctcatg cacagagtac tttactatgc gaaagattac gggtatacga
     6841 aatatacctc taccctcgct gaggcgtgga ggatctcaat caaaggactc tcggataccc
     6901 tcatttcagc cattcatatg cacggcgcac cgcctgaact ggatccggat gaaagagttg
     6961 gcgaggatcc tattgcagcc ttcggtatcc aagaagcccg gcgccacagg gcacgaggta
     7021 tcagtattgc catgttcctg ggtctgatga aatactaccg gcaaagctat atagatctgg
     7081 tcctggggtc atcattcggg gatatcgaga gagaactata tattcttttt gtaaatcgtg
     7141 tctttgaccg tgtggagatc gggttttgca gcgaatggtc tgcccaggaa aacgatgcca
     7201 gggttgatga gcttcaggca gctaaccgtt ccataacaaa tgaaaagaac aagtatctca
     7261 ctatttttga aagcattcac gagcccatct tcctgctgga ccgcgggaac cagatcgaca
     7321 acctcaacca ttccgcagct ttgcttctaa ccgggatgaa catacctggt tctcggtact
     7381 acgacgaaaa cccgaacaac cagtatctcc cgtggctgaa cgataaactg aaagagcttg
     7441 agacacacac ggatggggag agtgcctttg atattgacct tgaaacgctc gagggcgcgc
     7501 gctggtttca tgtcaggttg aagcggatgc tagacgtgag tgaaaagttc tggggaacag
     7561 tcgtcactct caatgatatt acaaagagaa aacaaatgga ggagcagctg catctccagt
     7621 tattaaccga tgaactgacc gggctttata acaggcgggg atttctggtc atggccgaac
     7681 agcacgtaaa gatggcgcgg cggctcaaga agggaataat tattattgcc gttgatatgg
     7741 atggtttgaa aaccatcaat gacaagtacg gccatcagga aggtgatgct gccattgcag
     7801 agacagcact aatcctgaag caatgctttc gtgagtcaga catcatcggc cgcatgggtg
     7861 gcgatgagtt cgcggtcctc ctccttgagg gcgtggacgt tcccccggag cagaccgtca
     7921 tcgaccgcct caacgcgcac ctccgcacca ggaatgagaa caaaggcagg gattatcagt
     7981 tatcgttcag taccggaatt ttccgctgtg ctccggaatg tataaagtct ctggatgagt
     8041 tgttggccag ggcagatgca ttgatgtacc agcagaaaca gagtaagagg accttatgag
     8101 cgtctgacaa tgtctatgtt cctcttggat tcctcactgc cactaaaggc ttaagggctt
     8161 cccatcagga atgaaagcgt ttcctatgtc tggatacaca acaaatgtca ttgtccatcg
     8221 cgctgtgctc gatgcagggg tgcatttcat ccagaaacct ttcgatatga acgagcttgc
     8281 ccgcaaggtc catgaggcga tcagaggcag ctggacgttc gcatacatgc gtgggaaagg
     8341 ggcaagcgct gttcagcccc agggcctcac ctccttccat cttccttttc tgaaccggat
     8401 agtcatggca atgccctgaa cgcacatgga tataaccatg gcgagccaga cgccgaaggc
     8461 gccgtatcct aagacaaccg cgagaagata cgcaaggggt aaacggataa tccagagtgc
     8521 gctcacgatg accagcatcg cccctttcgt atccccggca ccctggagac accctcccag
     8581 aataacgctg agcgccataa agggctcgga cagcatcgtg actcttaggt agcgcgtagt
     8641 ctcggcaagt acctgccaat cctttgccac cggtgaggta atgtcacccg cccatatgaa
     8701 gatcggcagc gccataatac tgacaaacaa gacccctgaa acagctatct tccatcctat
     8761 cttttgcgcc ctttctgatg cccctgcgcc aagattctga cccgtaagca ctgatgccgc
     8821 catgctgagg gcaaaagcgg gaaggtagat gatggcttcg atcctcagtc cgttggtgag
     8881 cgctgccatg gcagtgatac ccccttccag aagatggctt aggatattgt aaagcacaat
     8941 accgccggcg ttccacgata tttgtatgag ggctgcgggc cagctcagga gaactatctt
     9001 cctcacaaga tcagccgaca cataccatgg gccagaaaat atattcttcc agcggctctt
     9061 tctgaaaaga atcaggcata caatcgtgcc cgcggacatg gcggctgcag tggagaaggc
     9121 aatgcccctg tagccaaatc cgtgaaagga agacagtccg aagaccagga gaaaattcag
     9181 tccgatatta atcacactga tgcagaacat cgccaggagg ctcaggcgaa cctcacctcc
     9241 tgcacgaaag atcgcgttgg acagtatcac gaggtaattc ggcgaaaggg cgaaggcgaa
     9301 gatgataaaa aagtcatcgg cgatttgccg gacattcccg gaaaaacctg ccaaggacac
     9361 gatcttatca tgaaagagca gtccggcacc ggtgagcgca agggcgcacg caatgccgaa
     9421 aagaagggac tgttttgcta cagagagggc atcaccgaat ttgcccgcgc ccacagccct
     9481 cgagaccagc gccactgtgg caatgcttat ggcgtttgcc acaatgataa taaagaaata
     9541 gagctgggag acaaatccca cgatggcctg gatctcgggg ccgaggaagc cggcaacata
     9601 gatgtcggtg agacccacaa gaaaattgag aaacatcaca aggagcattg gccacgacat
     9661 tcgccaaatg ttttcccatg tgcttccagt agtaagatca atccaggcct tttccgtcac
     9721 caagacctcc gcacataatt gatccgccca ttctatctct catggcctgt gtctggaaaa
     9781 aggataaaca gaggcaaaag accggactag taaccagaaa cgatgaacta cttcagtgga
     9841 tgctgaacag gggaattgag gcggcaacgg acgatctgaa aaat
//
LOCUS       DATKOU010000090         3972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4350632, whole genome shotgun sequence.
ACCESSION   DATKOU010000090 DATKOU010000000
VERSION     DATKOU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4350632"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VMU21_04145"
     CDS             <1..294
                     /locus_tag="VMU21_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76749.1"
                     /translation="RRTPEDLKEFMKKQLTMLRAAAKMVKPGGSIVYSVCSTEPEEGE
                     EVVKEFLKDSDDFYIIDTTLPFLKGFMKEGLFRTYPHIHDMDGFFGVKLCKKD"
     gene            291..1031
                     /locus_tag="VMU21_04150"
     CDS             291..1031
                     /locus_tag="VMU21_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HUO76750.1"
                     /translation="MRKAVTIPLYVIGFILLGLVSGYLAFKALSFSKTVDVPDLRGKT
                     LVEANTLLTKKGLYIKVEGEDYDPLIVPGRIVRQDVPGGNKVKEQRGIKVFLSKGPKI
                     WSIPELAGQTIEEAEPVITKSGLRIEKIIRLHSDTIEKDRIIAQRPTPDEVAWGTSSQ
                     KSEESGERQYGLSLIVSSGPYEMIYSCPEFSGRSREEALALAERLRLNVQLSGSGERV
                     KSQRPKPHTLIKSGEPVYLQLEGEEIVR"
     gene            1028..1687
                     /gene="rpe"
                     /locus_tag="VMU21_04155"
     CDS             1028..1687
                     /gene="rpe"
                     /locus_tag="VMU21_04155"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HUO76751.1"
                     /translation="MIKIAPSILSADFTRLGEEIQAAESAGADMLHIDIMDGHFVPNI
                     TIGPFVVEAIRKTTALPLDVHLMIEEPDRYIADFIKAGADYLTVHLEASVHLHRTIHW
                     IKDSGAKTGVSLNPETPIGTLDYILPDLDLVLLMSVNPGFGGQGFIPQILGKIKALKG
                     LLAELGFPVLIEVDGGIKYENAKEVADAGADILVMGSAFFNSGDYQALTAKLRNLFIA
                     R"
     gene            1677..2765
                     /locus_tag="VMU21_04160"
     CDS             1677..2765
                     /locus_tag="VMU21_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76752.1"
                     /translation="MPDKILRRAELMRESSRYREALSLYIRAIHMYRKRGSKEGVLNA
                     LLAVGDMSRMLGDFPRAEGSYSDAILLAEQIGDRVIRADAVMGLGLSVRALGDWQRAL
                     RLFAEARGIYRKTSDRDGLAFALWAQGGAYRIGGKIKNAMGSFKSSQSIFKSLGTDPD
                     NKKGQAYSLCGLGGVSRVAGLFRDSMGYYTSANRLLSSLNDRFGVAYSHCGMGNALRM
                     RGNYKNALRHLKRAERIYLTIGDIVSYSYTLWSLGMVHLMEGRLRWAEHYVMKAFARF
                     RKTKDIRGLIYCRLTLGQVQYLRGHRTAARRELMKAFRDSVEKKFGIETCHSRAILDY
                     IEKGEKTHGCYRRTGSGLSVDSVPFNIP"
     gene            2846..3382
                     /locus_tag="VMU21_04165"
     CDS             2846..3382
                     /locus_tag="VMU21_04165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUO76753.1"
                     /translation="MAILNLPNTLTITRIVLIPLFVTSVVYLRYDYALYLFVIAALTD
                     MFDGLIARLKNQKTLLGNFLDPLADKFLLITSFVLFSLYGLIPTWLTITIISRDIIVI
                     TGWVVLYLLTHTAKVEPTMMGKLAITFQLLLICYILLDINLPAVPDIHGYLIWLTAVF
                     TTTSGLHYIYRGLKLANA"
     gene            3375..>3972
                     /locus_tag="VMU21_04170"
     CDS             3375..>3972
                     /locus_tag="VMU21_04170"
                     /inference="COORDINATES: protein motif:HMM:NF039894.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUO76754.1"
                     /translation="MHNNPGAEIASVLPESVAYAAGIAPGDIILSVNGHKVCDSIDFM
                     FFRNEEKLHVIAKRKDKRLSFPLVLKEGQDAGIELKPFKIKTCKNRCIFCFVHQLPNG
                     LRKTLYVKDEDYRMSFLYGNYITLTNLSPQDKKRIIQQKLSPLYLSVHSTSGTLRNTL
                     LGNPKAPDILKEIAFFKEHKIRMHCQVVLCPGYNDGKDI"
BASE COUNT         1039 a          974 c         1009 g          950 t
ORIGIN      
        1 cgccgcacgc ctgaggacct caaagaattc atgaagaagc agctcaccat gctgagggct
       61 gcggcgaaaa tggtaaaacc aggggggagt attgtctatt ctgtgtgctc aacagagcct
      121 gaggaagggg aagaggtggt aaaagagttc ttgaaggatt cagatgattt ttatattata
      181 gacacaacgc ttcccttcct taagggattt atgaaggaag ggcttttcag aacatatccc
      241 cacatacatg atatggacgg attcttcggg gtgaagcttt gcaagaagga ttgagaaaag
      301 cggtaacgat tcccctttac gtcatagggt tcattctcct ggggcttgta tcgggctacc
      361 ttgcgttcaa agccctgagt ttcagcaaga cggtggatgt gcctgacctc aggggcaaga
      421 ccctcgtaga ggccaatacc cttttgacca agaaggggct gtatataaag gttgagggtg
      481 aggactacga ccccctcatc gttccgggga ggattgtgag acaggacgtg cctgggggga
      541 acaaggtgaa ggaacagcga gggataaagg tttttctaag caaggggccg aaaatctggt
      601 ccatccctga gcttgcagga cagactatcg aggaggccga gccggtcatt accaagagcg
      661 gtttaagaat cgaaaagatc atccgtctcc attccgacac tattgagaag gacagaataa
      721 ttgcccaacg gcctactccc gatgaggtgg catggggaac ttcttcccaa aaaagcgaag
      781 agagcgggga acggcagtat ggtctctccc ttattgtgag ctcagggcct tatgaaatga
      841 tttacagctg tcctgagttt tccgggagaa gcagggaaga ggcactggcc cttgccgaaa
      901 gactccggct taacgtacag ctctcgggtt caggcgaaag ggtaaaatct caaaggccca
      961 aaccccatac tctcataaaa tcaggagaac ccgtctatct tcaactggaa ggagaggaga
     1021 ttgtcagatg attaagattg cgccctccat tctctctgcc gattttacaa ggcttggaga
     1081 agagatacag gctgcggaat ccgctggtgc ggatatgctc catattgata tcatggacgg
     1141 acattttgtt cccaatatta ccatcggtcc ctttgtggtg gaggcaatca ggaaaaccac
     1201 agcccttccc ctggatgttc atctcatgat tgaagaaccg gatcggtata tcgcagactt
     1261 catcaaggca ggggccgatt atctcactgt tcatctggag gcctccgtgc atctccatag
     1321 aaccattcat tggattaagg acagcggcgc aaaaacaggg gtctccctga atcccgaaac
     1381 ccccataggg actctcgatt acattctgcc ggatttggac ctcgtccttc ttatgtcggt
     1441 gaatcccggg tttggaggtc agggttttat tccccagatt ctcggcaaaa taaaggccct
     1501 caaaggcctg ctcgccgagc tggggttccc tgtgctcatc gaggtggatg gagggattaa
     1561 atatgaaaac gcaaaagagg tggctgacgc aggcgctgac attctggtta tgggctctgc
     1621 tttttttaat tcaggggact atcaggcact caccgcaaaa ctgaggaatc ttttcattgc
     1681 cagataagat tttacgccgc gcagaactca tgagagaatc ctcccgatac cgggaggccc
     1741 tctccctcta tataagagcc atacacatgt acagaaagag gggaagcaaa gaaggggtgc
     1801 tcaacgctct cctcgccgtg ggagatatgt ccagaatgct gggcgatttc ccccgggcgg
     1861 aaggttcata ctctgatgcc atattacttg ccgaacaaat aggagacagg gtaatccgtg
     1921 ctgatgcggt tatgggcctg ggcctctcgg tgagggcgtt gggagattgg cagagggcat
     1981 tgagattatt cgctgaagcc aggggtatct accgaaaaac gtctgacagg gatggtcttg
     2041 cgtttgcact ttgggcacaa gggggcgcct acaggatagg aggaaaaata aagaatgcca
     2101 tgggctcctt caaatcttcg cagagtatct ttaagtcatt aggcacagat cctgacaata
     2161 agaaaggaca ggcttacagc ctctgcggcc tgggcggggt ctcgcgtgtt gccggcctgt
     2221 tcagggactc catggggtac tacacctctg caaaccgctt gctatcctct ttgaatgacc
     2281 ggttcggtgt agcctattcc cattgcggta tggggaacgc attgaggatg agaggcaact
     2341 acaagaatgc gctgcgccat ctcaagaggg cagaacgcat ctatctgaca atcggtgata
     2401 tcgtgagcta ttcctatacc ctctggagcc tcgggatggt gcaccttatg gagggaagat
     2461 taaggtgggc cgaacactac gtgatgaagg cgtttgcgcg attcagaaag acaaaggata
     2521 tacggggtct tatctattgc agacttactc tcggccaggt acagtacctg agaggtcaca
     2581 gaaccgctgc acgaagggaa ctcatgaagg cgtttcgcga ttcagtggaa aaaaagtttg
     2641 gtatagagac ctgccactca agggcaatcc ttgattatat agaaaagggg gaaaagactc
     2701 acgggtgtta ccgaagaacc ggatcggggc tgagtgtgga ctccgttccc ttcaatatcc
     2761 cctaagagat tgggtgcact atgagaaccc atagaatgtg ctcctccggt tgtttcaatc
     2821 attaccctca tcattggaga agagtatggc tattctgaac ttgccgaata cgctcaccat
     2881 cacgagaatc gtccttatac cattattcgt gacctcggtc gtctatctga gatacgatta
     2941 cgccttatat ctttttgtca ttgccgcact taccgacatg tttgatgggc tcattgcccg
     3001 cctcaaaaac cagaaaacgc tcctgggaaa tttccttgac ccccttgcag acaaatttct
     3061 tcttatcacc tcctttgtcc tcttttccct ctacggcctg attcccacat ggcttaccat
     3121 cacgatcata agcagagaca tcattgtgat caccggctgg gtcgtccttt atctgctcac
     3181 ccatacagca aaggtcgagc ctacgatgat gggtaagctt gccatcacat tccaactcct
     3241 ccttatctgc tacatacttc tcgacatcaa cctgcctgcc gttcccgaca ttcacggata
     3301 cctgatatgg cttaccgcag tgtttacaac aacctcagga ttgcattata tttacagggg
     3361 gcttaaactt gccaatgcat aacaatcctg gtgccgaaat agcgagcgta cttcccgaaa
     3421 gcgttgccta tgctgcggga attgccccag gagacataat cctctccgta aacggccaca
     3481 aggtctgtga tagcatcgat ttcatgtttt tccggaacga agagaaattg cacgtcatcg
     3541 caaagaggaa ggacaagcgc ctctcatttc cccttgtgct caaggaggga caggatgcgg
     3601 ggatagagct taaacccttt aagataaaaa cctgtaaaaa caggtgcata ttctgttttg
     3661 tgcaccagct tccgaacggc ctgaggaaga ccctgtacgt gaaggacgaa gactaccgca
     3721 tgtcattcct ctatggcaat tacattaccc ttaccaacct ttcccctcag gacaaaaaaa
     3781 gaatcataca gcagaaactc agcccccttt acctctctgt ccattccacc agcggcacgc
     3841 ttcgcaatac cctgctggga aaccccaagg cacctgacat cctcaaagag atcgccttct
     3901 tcaaagaaca caagatacgg atgcattgcc aggttgtcct ctgtcccggt tataatgatg
     3961 gcaaagacat cg
//
LOCUS       DATKOU010000091         9109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2424509, whole genome shotgun sequence.
ACCESSION   DATKOU010000091 DATKOU010000000
VERSION     DATKOU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424509"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VMU21_04175"
     CDS             <1..644
                     /locus_tag="VMU21_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUO76755.1"
                     /translation="YVSMGNYVFNRDVLLDSLAKAQRKNQHDFGAHVIPDLVETGKVF
                     AYDFATNVILGSKPYEEKGYWRDVGTIAAFFDAHMDMLRDLPLFDLHNRSWPIHPSSH
                     EHPATKILGGNIQCSLIAEGTIIHNAKIRNSVIRSDVVIEDDVSVEDSLIMDHSVLRR
                     GCRLKRVIVDKHNVVKAGEEIGFNPDADRLSCHIDVSGIAILPRGGRPSKDVR"
     gene            644..1006
                     /locus_tag="VMU21_04180"
     CDS             644..1006
                     /locus_tag="VMU21_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76756.1"
                     /translation="MEKDILSKVIEVEKEIQERLREEKEKSHEWLEGVKTEIERDISL
                     QEEQLRESYTRGREDAKAEAEREAERIVKDAATLAERYRQIGDETLEKIVKKHVVKIL
                     PAHSTKSPSTGESSGTIP"
     gene            1003..2886
                     /locus_tag="VMU21_04185"
     CDS             1003..2886
                     /locus_tag="VMU21_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HUO76757.1"
                     /translation="MIVRMSKIEIVGPKGLLEDVLSLLKTLGIFQIEPATLGFIEKGC
                     EEHVRSFLPEEQALSEMIFFEDLRARIGELLLYLPAIEVRISYIEPLPIIETVSKTIQ
                     KHISICKDLYQRRDNLTKEANELSRYTIFLSTLSTLIESGQETPDLDFVGLTIKEPAM
                     VGRLRDLLSRLTDFRFELLTETAEDGTLVGLITIEKDFSEKVKRSLTDERIPELSFPQ
                     SISNLTFSEKCAYVRKRVLDLNAEIASITRELERFSRRWLPIYNRVKEWLDEKLSLLK
                     TSASVFETRMCFFINGWIASRNMEPLRGRLTEAFKGRVTLEEKGMHEEDLERVPIILK
                     NPAYFQPFELLVKLLPLPKYTSYDPTPFIGIFFPIFFGMILGDAGYGVLLLALSLIMM
                     RRWKRRCDLRDGCKILSVASLYTIFFGILYGEYFGELGNELLGIEPLFMDRRTAIIPI
                     LLFSLSVGVMHVTLGLFLGFLSAMRQKIRKEAAFKLLSIVVIFCVVILIVSLFKPFPW
                     LVTRPLIIGILILTPLLLFTGGLLAPLELLKSIGNIVSYARIMAIGLASVLLAYVANR
                     MAGMMGDVVVGIIAAVLLHIINIVLGVFSPTIHSMRLHYVEFFSKFVEHGGRRFEPFK
                     KGN"
     gene            3014..3232
                     /locus_tag="VMU21_04190"
     CDS             3014..3232
                     /locus_tag="VMU21_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76758.1"
                     /translation="MEKVLVAFAAALAIGLSAIATAWAQSRIGAAGAGTLAEKPEMSG
                     TIIILLAIPETMVILGFVIATMILFTVK"
     gene            3234..3824
                     /locus_tag="VMU21_04195"
     CDS             3234..3824
                     /locus_tag="VMU21_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HUO76759.1"
                     /translation="MSYRELIDALQRECEEKIRKIWQEAEGEAARAKADAAKRIGEVR
                     EGRERNQSAALKVLTETILSEAEGRARVSRLLAEKELSDRLYRIALNSLHHLRGEGYK
                     DIFSALVNELPQCNWGMVKVNPQDRVFAETLFPNDEVVSDPAVTGGLEVNEKEGSLRV
                     VNTLDKRLERIWSEILPEMTEEVRSMMEKEKQSLTV"
     gene            3843..4805
                     /locus_tag="VMU21_04200"
     CDS             3843..4805
                     /locus_tag="VMU21_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HUO76760.1"
                     /translation="MQLLQEIEDRGYPTEYLLSRIRGRRVSLISDWEPLLLGAATAAS
                     IPLHHRDFAPDTSSQGEWRHLLREFRWVYFQMNKRLGQIFSPFFLYAELRTLFFFLRY
                     RTTNEQTKWEQLLSVSLLSKRSKMRLKRSEDIQDAMSMVEELLVSYSSIFRGMGETFI
                     KEGLRGMERWLTNRYLEHAVTLSLHPVIKKFFVRTIDSRNLLTLYKRLRWDLKGEADF
                     VKGGSITVHKLRAADDRGDMFNALRMIQAFTGVTVQNPDATSVEKAVYTATTHTVRRE
                     GREPSGVALILDYLWRCSMEIRNLGVVIHGEGIDRDILRTELIL"
     gene            4802..5122
                     /locus_tag="VMU21_04205"
     CDS             4802..5122
                     /locus_tag="VMU21_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HUO76761.1"
                     /translation="MKKIVCLTPRGEEYGFSLAGTNHYTIGAEELEDTLKTLMLDAET
                     GLVVIDERLIKGITREKLREMEQRWYGILLVLPVPEKLGVEVEDYAMSLIKRAIGYHV
                     RLKL"
     gene            5119..6729
                     /locus_tag="VMU21_04210"
     CDS             5119..6729
                     /locus_tag="VMU21_04210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HUO76762.1"
                     /translation="MMGRIKGISGPTVSVDMKGLKLYDRVYVGHSMLTGEVVRLDQDV
                     AVIQVYENTKGLGIGEPVKSVNMPLTVRLGPGLLHGMFDGLQRPLEILREKMGSFISS
                     GTEIYALDYLKKWSFSPLKKKGEPVTRGEIVGYVEEGPFKHFIFSNFEGRISRIMGGE
                     LSLNDTIAELEGGKEIFACNEWPVRMPRPYRRRLSTWEPLLTGQRVIDLLFPLARGGT
                     AIFPGGFGTGKTILEQAIAKFANTDIVVYVGCGERGNEMAEMIGEFESLKDPWTGKYL
                     MERTVLVVNTSNMPVAAREASIYTAVTMAEYYRDMGYHVLLLADSLSRWAEALREISS
                     SLEEMPGEEGYPTYLASRLAGFFERAGVVETLSGRIGSLTMILSVSPPGGDFTEPVTQ
                     ACLRTAGAFFMLDTSLAHRRHFPAINWFQSYSLYEKEISRHFIEHVSPQWETLKERCR
                     ELLKREESLREVAEIVGSEGLQDADRLLMKVAEMIRMEFLCQNAYTEDAFSPPEKTIA
                     KMGEMVEYYESAMAKLKQGISLEETLKG"
     gene            6758..8080
                     /locus_tag="VMU21_04215"
     CDS             6758..8080
                     /locus_tag="VMU21_04215"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HUO76763.1"
                     /translation="MRLSEYIYSTISSIRGPLLFVEKVFNARVGEVVRIRFPEGKEME
                     GEVLKIDGDTVLIQAYGETRGLDTGNSSVVFTDSVKRAPLSPDVIGRVFSGSYAPIDG
                     APMFVPEKWVPVTGYPINPASRARPEEFIETGFSCIDGLNTLVKGQKLPIFSSAGLPS
                     KEVAAVILKNSRLGSETGGFVVVFVAVGLTYYDYSFYTRVLEEMRTGFVAFANMADEP
                     VVERLLAPRFGLTVAEYLAFEKGMDVLVVITDMTNYCDALREVSTAREELPGRRGYPG
                     YMYSDLASLYERAGRIKGMTGSVTMLPVVTMPEDDITHPIPDLTGYITEGQLVLSRDL
                     HQKGIFPPMDVLPSLSRLMQSGIGKGHTREDHRKTANLLYKYYAKGRDLRRLEAIVGR
                     DGMAEADRFMLDFADSFEKEFVSQGMVRRNIQETLDTGLNLLRRFSLT"
     gene            8103..8498
                     /locus_tag="VMU21_04220"
     CDS             8103..8498
                     /locus_tag="VMU21_04220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUO76764.1"
                     /translation="MGPFSIEKTIYYHDTDCGGVVYYANYLKYMEEARTEYFRTRGIE
                     LPKLAENNIWFVVARVEIEYKSPARYQDRLRIFVTVQEARLSTIKFSHEIRKGEKTVA
                     KSTTTLVCVGGDFRPAPLPEEVKKALKDT"
     gene            8627..>9109
                     /locus_tag="VMU21_04225"
     CDS             8627..>9109
                     /locus_tag="VMU21_04225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO76765.1"
                     /translation="MRTTTKVWDKRFKKDGKVFEQPHEDMPGIISLLKEKGARTVLDL
                     GSGSGRHLVSLARSGFSVFGLDNSLEGTRIAKNWLKHEGLSAEFHVQEMADPLPFPDS
                     FFDAVISIQVIHHARPMTIEKIVKEVARVLKKGGFVFITVPKEKSQAKKFVEIEPCTF
                     I"
BASE COUNT         2357 a         2065 c         2585 g         2102 t
ORIGIN      
        1 cctatgtgtc aatggggaat tacgtattca acagggatgt gctcctggat tcactggcaa
       61 aggcgcagag gaaaaaccag catgatttcg gggcccatgt gatacccgac cttgtggaaa
      121 caggcaaggt attcgcctat gactttgcca ccaatgtcat tctgggctca aagccctatg
      181 aggagaaagg atactggagg gatgtgggaa ccattgccgc gttctttgac gcacatatgg
      241 acatgctgag ggacctcccc ctcttcgacc tccacaaccg gtcgtggccc atccaccctt
      301 caagccatga gcaccctgcc acaaagatac tgggagggaa catacagtgc agtcttatcg
      361 ctgaggggac cataatccat aacgcgaaga tacgcaattc ggtgatccgg agtgacgtgg
      421 tgattgaaga tgacgtgagc gtggaggatt ccctcattat ggaccattcg gtgctcaggc
      481 ggggctgccg cctcaaaagg gttattgtgg ataaacacaa tgtggtgaag gctggtgagg
      541 agatcggttt caatcccgac gcggaccgcc tctcatgcca tatcgacgta tcagggatcg
      601 ccatactccc acggggggga aggccctcaa aggacgtccg gtaatggaaa aagatatcct
      661 gagcaaggtg atcgaggtcg agaaggaaat tcaggagagg ctcagggagg agaaggaaaa
      721 gtcccatgag tggctggaag gggtaaagac ggaaatcgag agggacattt ctctgcagga
      781 agaacagtta cgggagtcat ataccagggg gagggaagac gcaaaggcgg aagccgaaag
      841 agaagcggaa agaatagtga aggatgctgc tacgctcgcg gagcgatacc gtcagatagg
      901 cgatgaaaca ttggagaaga tcgtgaagaa gcatgtggta aaaatactcc ccgcgcacag
      961 caccaaatcc ccttcaacag gagaatcttc agggacaatt ccatgattgt acggatgtcg
     1021 aagatcgaga tcgtggggcc taagggcctt ctggaagacg tgctttccct tttaaagacc
     1081 ctcggtatat tccagatcga accagccacc cttggattta ttgaaaaggg gtgcgaagaa
     1141 catgtcaggt cctttcttcc ggaagaacag gcactctcgg aaatgatttt ttttgaggat
     1201 ctcagggcga ggattggaga gctcctcctc tatctccccg ccattgaggt gaggataagc
     1261 tacatagaac cgctgcccat catagaaacg gtgagcaaaa cgattcagaa acatattagc
     1321 atatgcaaag acctgtacca gcggagggat aacctcacaa aagaggcgaa tgagctcagc
     1381 cgctatacaa tattcctctc aacgctctca acccttatcg agagtggaca ggagacccct
     1441 gatcttgact ttgtgggtct caccatcaag gaacccgcaa tggtgggccg tctcagggac
     1501 ctgctttcac ggctcaccga tttcaggttt gagctcctga cagagaccgc agaagacgga
     1561 acgctcgtgg gactcattac catagagaaa gacttctcgg aaaaggtgaa gcgctctctc
     1621 accgatgaac gtatccctga actgagcttc ccccaatcca taagcaatct caccttctca
     1681 gagaagtgcg catacgtcag gaaaagggtt ctcgatctca atgcagagat tgcctccatc
     1741 acccgggaac tggagaggtt ttcacggcgg tggctgccta tctataacag ggtgaaggaa
     1801 tggctggacg agaagctctc tctgctcaag acctctgcct cggtctttga gacgagaatg
     1861 tgtttcttca tcaacggatg gattgcttcc aggaatatgg agccgctgag agggcggttg
     1921 acagaggcgt ttaagggaag ggtaacactg gaggagaagg ggatgcatga agaggacctg
     1981 gagagggtac ccatcatcct caagaatccg gcctattttc agccttttga gctccttgtg
     2041 aaattactcc ccttgccaaa atatacctcc tatgacccga cccccttcat agggattttt
     2101 ttccccatct ttttcgggat gatcctgggc gatgcagggt atggggtgct cctccttgcg
     2161 ctctccctca ttatgatgag acgctggaaa aggagatgcg acctgaggga tgggtgtaaa
     2221 atcctgtctg tcgcatccct ctataccatc ttcttcggta tcctctatgg tgaatatttc
     2281 ggtgaactcg gcaatgaact tcttggtatc gagcccctgt tcatggatag gcgaactgcc
     2341 attatcccga tactcctttt ttcactcagc gtgggcgtca tgcatgtcac cctgggactc
     2401 ttcctgggat tcctgtctgc aatgaggcaa aagatccgga aggaggctgc gtttaagctt
     2461 ctcagcattg tggtgatctt ctgtgtcgtc atcctgatcg tgtccctgtt caagcccttt
     2521 ccctggctgg tcacgaggcc gctcattata ggaatactca tccttacccc tctcctcctt
     2581 ttcacgggag ggctcctcgc acctctggag cttctcaaaa gtatcggcaa tatcgtctcc
     2641 tatgcgagga ttatggccat cgggctcgca tcagtgcttc tcgcctatgt ggcgaacaga
     2701 atggcaggaa tgatgggtga tgtcgtggtc ggcattattg ccgcggtact gctccatatt
     2761 atcaacatcg tgctcggcgt cttctctccc actattcact ccatgaggct ccattacgtg
     2821 gaatttttca gtaagttcgt ggagcacgga ggcaggagat ttgaaccgtt caaaaagggg
     2881 aattgaatgt ggagaataaa tgcggaggaa gaattcggaa ttcagaggaa tgggtgagag
     2941 tgacgtttgc ccgtgagata tgctcctgaa ttcttggtat aaaccatttt ttctgataag
     3001 gagggaaaaa ggtatggaaa aagtattggt ggcctttgcc gcggccctgg caatcggact
     3061 ttccgccatt gccactgcat gggcgcagtc aaggatcggg gcagctggtg cagggaccct
     3121 tgcggaaaag ccggaaatga gcggcacgat cattatcctt ttggcgattc ctgaaaccat
     3181 ggtgatcctt ggctttgtga tcgcgaccat gatactcttt acggtcaagt aaaatgagtt
     3241 atagagaact cattgatgcc ctccagaggg aatgcgagga gaaaatccgg aagatctggc
     3301 aggaagcaga gggtgaggct gcaagggcca aggccgatgc agcaaaaagg attggggaag
     3361 taagggaagg ccgcgaaagg aatcaatcag ctgccttaaa ggtcctgacc gaaacaattc
     3421 tttcagaagc agaggggagg gccagggtga gcaggctctt ggccgagaaa gagttgtcgg
     3481 atcgcctgta taggatcgcc ctcaattccc tccatcactt gaggggtgag ggatataagg
     3541 atattttttc tgcgctggtc aatgaacttc ctcagtgcaa ctggggaatg gtaaaggtaa
     3601 atccccagga cagggtattt gcagagaccc tcttccctaa tgatgaggtg gtatcggacc
     3661 ctgcagtcac aggaggcctt gaggtgaacg agaaagaggg cagtctgcgg gtcgtcaaca
     3721 cccttgacaa gagactggag agaatatggt cagagatact gcctgaaatg acagaggaag
     3781 tgagatccat gatggaaaaa gaaaagcaat cattaaccgt gtaatatatc cgggcgagga
     3841 atatgcaact ccttcaggag attgaggaca ggggatatcc cacagaatac ctcctttcga
     3901 gaataagggg gaggagggtc tcccttatct ccgactggga acccctcctc ctgggagccg
     3961 ccaccgcggc gtccattcca ttgcatcata gggactttgc tcctgatacc tcttcccagg
     4021 gcgaatggag acaccttttg agggaattca ggtgggtgta ttttcaaatg aataagagac
     4081 tcgggcagat cttctctcct ttttttctgt atgccgagct caggaccctt ttcttcttcc
     4141 tcagatacag gacgaccaat gaacagacca agtgggagca gctcctttcc gtgagtctcc
     4201 tctcgaagcg gtctaagatg cgtttgaaaa ggagtgaaga cattcaagat gcgatgagta
     4261 tggtggaaga gctccttgtg tcctactcat caatattcag ggggatgggg gaaaccttta
     4321 tcaaggaggg attaagaggg atggagcggt ggctgaccaa tagatatctt gagcatgcgg
     4381 tgacgctaag tctccatcct gtaataaaga agttctttgt ccgcactatc gactcaagga
     4441 atctcctcac cctttacaaa cggcttcggt gggatctgaa gggtgaagcg gattttgtaa
     4501 agggcggcag tatcactgta cataaactga gggcagctga tgatagaggg gatatgttca
     4561 atgcgttaag aatgattcag gcattcacgg gagtgactgt gcaaaacccc gatgccacaa
     4621 gtgtcgaaaa agccgtctat acggccacga cacacacggt gagaagggaa gggagagagc
     4681 cttcaggggt tgcccttatc cttgattatc tttggagatg ctccatggag atcagaaatc
     4741 tgggtgtcgt gattcacggg gaagggattg acagggacat cctgaggaca gaactgatac
     4801 tatgaaaaag atcgtctgtc tcactccgcg gggggaagag tatggattca gccttgcggg
     4861 aacgaatcat tatacaatcg gagcagaaga actggaggat acgctgaaga ccctgatgct
     4921 tgatgcagaa accggacttg tggtcattga tgaacgattg atcaagggta ttaccaggga
     4981 gaaactgaga gaaatggagc agagatggta cgggatcctc ctcgtccttc ccgtgccaga
     5041 aaagctgggc gtggaggttg aggattatgc catgagtctc atcaaaagag ccataggtta
     5101 ccatgtgagg ctgaaattat gatgggccgc ataaagggca tttcaggacc caccgtgagt
     5161 gtggacatga agggtctgaa gctctacgac agggtgtacg tgggacattc catgcttacc
     5221 ggtgaggtgg taaggctgga tcaggacgtt gcagttatcc aggtgtacga aaatacgaag
     5281 gggctgggga ttggcgagcc ggtgaagagc gtgaacatgc cgctcaccgt gagattgggg
     5341 ccgggtcttc tgcacgggat gtttgacggg ctccagaggc cactggaaat cctgagggaa
     5401 aagatggggt cttttatctc ctcgggaacg gagatctatg cactggatta cctgaagaag
     5461 tggagcttct ctcccctcaa gaagaagggt gaaccggtaa cgcggggcga gatcgtcgga
     5521 tacgtggaag agggtccctt caaacatttt atcttcagca acttcgaggg caggatttca
     5581 cggatcatgg gtggagaact cagccttaac gacaccattg cagagctgga aggcgggaaa
     5641 gaaatctttg cctgtaatga atggcctgtg aggatgccga ggccctacag gaggaggctc
     5701 tcgacctggg agccgctttt gaccggccag cgcgtcattg atctgctgtt ccctctggca
     5761 aggggaggga ccgccatttt ccccgggggg tttggcacag gcaagaccat tctggagcag
     5821 gctatcgcaa aattcgccaa taccgacatt gtggtgtatg tgggatgcgg agagcgcggg
     5881 aacgaaatgg cagaaatgat tggggagttt gaatccctca aggacccgtg gacaggaaaa
     5941 tacctgatgg agaggacagt gcttgtggtc aacacctcca atatgccggt ggctgcgagg
     6001 gaggcatcca tctataccgc agtcaccatg gctgaatatt accgggacat ggggtaccat
     6061 gtccttctcc tcgctgatag cctttcgaga tgggccgagg cactgaggga gatatcttca
     6121 tccctagagg agatgcctgg tgaagagggg tatcccacct atctcgcgtc aaggcttgcg
     6181 ggatttttcg agcgggcagg ggtagtcgag acattaagcg gcaggattgg ttctctcacg
     6241 atgatcctgt cggtatctcc ccccggaggg gactttaccg agcctgtgac tcaggcgtgt
     6301 ctcaggaccg caggggcgtt cttcatgctg gatacgtccc ttgcccacag gaggcatttt
     6361 cctgcgatca actggttcca gagctattcc ctctacgaaa aggagatttc ccgccatttc
     6421 attgagcatg tatccccgca atgggaaaca ctgaaggaga ggtgcaggga actccttaag
     6481 agggaggagt cattgaggga ggtggctgag atcgtgggat cagagggcct tcaggatgca
     6541 gacagactct tgatgaaagt ggcagagatg atacggatgg agtttctctg tcagaatgcg
     6601 tataccgagg atgccttctc tcccccggaa aaaacaatcg cgaagatggg agaaatggtg
     6661 gagtactatg aatcagcgat ggcgaaactc aagcagggga tttcccttga ggagacgctt
     6721 aagggatgaa catcttacta atgacaggga atagcgcatg agactttcgg aatacatcta
     6781 ttcaaccatc tcttccataa gagggcctct tctctttgtg gaaaaggtat tcaatgcgag
     6841 ggtgggggag gtggtgagga tccgcttccc tgagggaaaa gagatggaag gggaggtgct
     6901 gaagatagat ggggatactg tcctcataca ggcctacggc gagactcggg gcctcgatac
     6961 agggaactct tctgtggtgt tcaccgattc tgtgaagagg gcgccccttt cccctgatgt
     7021 tatcggccga gtgttcagcg gctcctacgc gcccattgat ggcgctccca tgtttgtccc
     7081 tgaaaaatgg gtccccgtga ccggatatcc cattaatccc gcgtcaaggg caaggcccga
     7141 agaattcata gagaccggat tttcttgtat cgatgggctc aatacccttg taaagggtca
     7201 gaagctgccc atcttttcga gtgcagggct gccttccaag gaggtggcag ccgtgattct
     7261 caagaactca cgcctcggca gtgagaccgg cggttttgtg gtggtttttg ttgcggtggg
     7321 actcacatat tatgattact ccttttacac gagggtcctt gaggagatgc ggacaggctt
     7381 tgtcgccttc gcgaacatgg ctgacgagcc tgtggtggag agactcctcg cacccaggtt
     7441 cgggctcacc gtggcagaat acctcgcctt tgaaaagggc atggatgtcc ttgtggtgat
     7501 cactgacatg accaactatt gtgatgccct gagggaggtt tcgaccgcga gggaggaact
     7561 gcccggaagg agggggtatc ccgggtatat gtattctgac ctcgcctctc tttacgaaag
     7621 ggcagggagg ataaaaggca tgacaggttc tgtcaccatg cttcctgtgg tgaccatgcc
     7681 tgaagatgat atcacccacc caatccctga tctcaccggg tatatcactg aaggacagct
     7741 ggtcctgagc agggaccttc accaaaaggg catattccct cctatggatg tgcttccaag
     7801 cctttcgcga cttatgcaga gcggcattgg caagggccac acaagggagg accaccggaa
     7861 gaccgccaat cttctctata agtattatgc caagggaaga gacctgagaa ggcttgaggc
     7921 aatagtggga agggatggga tggccgaggc agacaggttt atgctcgatt ttgccgattc
     7981 ttttgagaag gaatttgtca gtcagggtat ggtgaggagg aatatccagg aaacgcttga
     8041 cacagggttg aatcttttga gaagattctc ccttacatag agtggcggag gtttgaaaag
     8101 gaatgggccc tttctccata gaaaagacaa tttactacca cgataccgat tgcggaggag
     8161 ttgtgtatta tgccaattac ctgaagtaca tggaggaagc ccgcacagag tattttcgca
     8221 ccaggggaat cgaactcccg aaactggcag agaacaatat atggtttgta gtcgcccggg
     8281 tagagataga gtacaaatca cccgcacgct accaggacag actcaggata tttgtgacag
     8341 tgcaagaggc gaggctctcc accataaaat tctcccatga aatcaggaag ggtgaaaaaa
     8401 ccgtggctaa atccactacg accctggttt gcgtaggagg tgatttcagg cccgcaccac
     8461 ttcctgagga ggtaaaaaaa gcattaaaag atacttaatt actggttggt tataatagtt
     8521 atataaagta taactttaat ataatcatgc atcggctttc ctcacttatc agaatcctat
     8581 gcgagatctt ccattaccct aattcattcg caaagaatat cagaacatga gaaccaccac
     8641 taaagtgtgg gacaaaaggt tcaagaagga tgggaaagtc ttcgaacaac cccatgagga
     8701 tatgcccggt ataatctcct tattaaaaga gaaaggtgcc agaactgttc ttgacctcgg
     8761 aagcggctca ggacggcatt tggtatccct cgcaagaagc ggcttttccg tttttggcct
     8821 cgataattcc cttgaaggga cccgcatagc aaagaactgg cttaagcacg agggactttc
     8881 cgctgagttt catgtacagg agatggcaga cccgttgcca ttccctgaca gcttttttga
     8941 cgcagtaata tcgattcagg tcattcatca cgccaggcca atgactatag aaaagattgt
     9001 gaaggaagtt gcgagggttc tcaagaaagg cggatttgtt ttcataactg tcccgaagga
     9061 aaaaagccag gcaaagaagt tcgtcgaaat tgagccctgc acatttatc
//
LOCUS       DATKOU010000092        11520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3197416, whole genome shotgun sequence.
ACCESSION   DATKOU010000092 DATKOU010000000
VERSION     DATKOU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197416"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..3588
                     /gene="fdnG"
                     /locus_tag="VMU21_04230"
     CDS             409..3588
                     /gene="fdnG"
                     /locus_tag="VMU21_04230"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1042..1044,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HUO76766.1"
                     /translation="MNVSRRNFLKVSGGTMLLGSLGINLDPVKAYAQTLRIKDAKESY
                     TICPYCSVGCGITAYVKDGKIINTEGDPEHPINEGTLCPKGASLYQMANNPLRVTKPL
                     YRAAGAKEWKEVEWDWALDQIARKVKDTRDRTFRERAKSKVKEKKTEPGTKEGGLPVE
                     VEIDVEKEFVVNRTDGIAHVGSAALDNEECYLLQKMLRSWGVVYIEHQARIUHSPTVA
                     ALGESFGRGAMTNHWVDLRNADVILVMGSNPASNHPIAMKWVMKAKEKGAKLIVVDPR
                     FTQTAAKADIYAPLRSGTDIAFINGMIKYIIDNNLIFRDYVVNYTNATCLVNPDFKLP
                     VELDGVFSGFDEKTAKYDKKTWTFQMDEKGLPKRDLTLKDPRCVYQILRKHVSRYTPE
                     MVSNITGTPKEKLIEVYKTFASTGKRDRAGTECYAMGWTQHTVGVQNIRAFCIVQLLL
                     GNMGIAGGGINAMRGESNVQGSTDYGLLFHILTGYNPTPSASLVDLKAYNEKFTPKTK
                     EPKSVNWWGNRPKYMTSYLKAIYGDKATKDNDYGYGWLPKLDEGMNASWLRLFLAMGM
                     GNFEGFFAWGQNPACSSAAAAKVRQVFTKLKWLVNVNLYDNETASFWKGPDMKPEEIQ
                     TEVFMLPCCSSVEKEGSISNSSRLAQWRTAAIKPIGQSMPDSEIMNELYYRVKKLYQK
                     EKGKFSAPILNLTWDYGEKNKDGKIEHFDIHRVAKEINGYYLEDIYDKKVDPPKLIGK
                     KGQLVTNFVTLQADGTTSCGNWLYSGSYTQKDDKVINMMARRGKEDPTGLGLYPNWAF
                     AWPLNRRIIYNRASVDPNGNPWDPKRAVLKWNPTKVNPATNKPGVWEGDVPDGPAPPL
                     SDEKGGKLPFIMKPDGIASIFGPGLADGPFPEHYEPLECPIQENPMSKKRRINPTAHP
                     IAQMAAHFSCDVRYPFVASTYRVTEHWQTGVMTRHTPWLLEMQPQMFVEMSKELAAEK
                     GIRNGDKVKVSSARGSLSAVAVVTERLKPFKVMNQTIHQVGMPWHFGWQFPVDGSGGD
                     SANLLTPFIGDPNTSIPESKAFMVNVEKA"
     gene            3600..4367
                     /locus_tag="VMU21_04235"
     CDS             3600..4367
                     /locus_tag="VMU21_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO76767.1"
                     /translation="MTRMGILVSPELCIGCRACQVACKSWNQLPGDKTVNRGGFENPP
                     DLTPHLYNKIHYLEVPAATDVQRWLFVSQRCMHCDDAGCMKICPSPGALYKTKEGAVA
                     INKDKCIGCKLCVAGCPFNVPRYDEKDKISKCHLCYDRIANGLQPACTKTCPTGALKY
                     GERDALISGAQKAGYAKLYGQTDLGGLGSLYAFKDAPKVYGMDERPAIPETIVFWHKV
                     LKPLSYIGLGGVVAASLIHYVAIGPHKEKEEEEKKNG"
     gene            4360..4974
                     /locus_tag="VMU21_04240"
     CDS             4360..4974
                     /locus_tag="VMU21_04240"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HUO76768.1"
                     /translation="MDRMIKKAGAFEIINHWVMAISCILLAVQGYGFLFHVEGVGSVF
                     GGFNSMRNLHNYLGIAFSISLFFSIFMYLKEALTFDADDIGWIMVLGGYLSHKVTVPP
                     MGKINTGQKFFYLAVLIFGIGISASGYVIWLLPGNKQYILLSHIVHNISFVVLMIAIP
                     AHIYLGTLANPGTLRIMLSGTVPYQWAKKRHPKWVAEVEGGHKA"
     gene            5082..5234
                     /locus_tag="VMU21_04245"
     CDS             5082..5234
                     /locus_tag="VMU21_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76769.1"
                     /translation="MKKLLILVVLFLASSLSSAQDKRYEVPVGDSPGYGQANAPVTMI
                     EFVDYQ"
     gene            5244..5672
                     /locus_tag="VMU21_04250"
     CDS             5244..5672
                     /locus_tag="VMU21_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUO76770.1"
                     /translation="MGVGPTVDKLLKDYKGKIRLVVKMNPYKYRDFSHIAAEAVLAAR
                     DQGKFWEMHQLLLKKSPQLDRTSLIKYAQESGLDVKKFTESLDTKKNASAIERDKQLA
                     ISLDLYETPVFFINGRKVTGNRAYDYFKKIINEEMKNEKR"
     gene            5659..6738
                     /locus_tag="VMU21_04255"
     CDS             5659..6738
                     /locus_tag="VMU21_04255"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUO76771.1"
                     /translation="MRKGRLSFLPSFAVMWGIILFVLTLDVHAGHLEPFKFVPIPQHN
                     PQTPEKIDLGKKLFFDRRLSGDGTMSCASCHIPELAFTDGQDISLSYPTTKNWRNSPT
                     LINAAFYKFLFHDGRVRTLEDQALFPMMSAFEMNKNLDYLEEQIRVVPEYAEAFKKVF
                     GGEVTRKRIAMAIASFERTLVSLNSPLDRYLKGEKGALSEGAKKGFEIFKGKGKCADC
                     HHGVNLSDDKFYALEVPENPVLLNDPRVTATMRFVAKVYHYDDYKNLTEDPGRYLITK
                     NKKDWKAFRTPTLREISKTAPYMHNGVFKTLDEIIDFFDSGGGKGNRALKPLGLSAED
                     KTDLKSFLVEALNGEEIPIQYPEIP"
     gene            6760..7512
                     /locus_tag="VMU21_04260"
     CDS             6760..7512
                     /locus_tag="VMU21_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HUO76772.1"
                     /translation="MNIEEIIQEKPHLKETILLYEKVLTFDSMVAKMTNVITLDEVTY
                     PPEIADAVVERFSSNFGIPEEHLLPFKEALKMGQLDFTRLPLNEIPSFSFPYHEDELG
                     LILFLMSKPFFLRLGEMYHLSGSTWEEGRCPVCHAKPSLASIDSENRRKLYCSFCGTK
                     GYYTRHGCPLCSNEDTSKITLFTFDQEKDFRIDACHQCKSYVKTVLNESIIENRLTPD
                     LADLMSLPLDVIAQGKGFQRHSPNPIGMTKMG"
     gene            7635..9002
                     /locus_tag="VMU21_04265"
     CDS             7635..9002
                     /locus_tag="VMU21_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM family putative metalloenzyme
                     maturase"
                     /protein_id="HUO76773.1"
                     /translation="MFLRDDEPRGSHSNELEEIQNSSLKSRTYPSKLFVEVTTRCNLR
                     CAMCVKQNTSTGMSEGNISLETFRALEPSFPHLSALILNGVGEPLLYPQLEEFIRRAK
                     KQLPHNAWVGFQTNGVLLNRERALSLVEAGLGRICLSMDAISRDTFRRIREGGEVRDL
                     ESAFSALNAAVKSANGHTRRRLDIGIEFVLMRENLFELPQTVRWAAHRRATFALVTQL
                     LPCTKLGTNQAVYDTNTHGAISVYDLWKNRAGLQGLDIRRYFDVFMKHFKNADEENIF
                     ALIEEMKADAGSRGITLHLERLLLRDEEWLKRVEEIFEETLEIGQEEGIEVKLPEIAP
                     RNNRKCEFVEGESSFVSWEGDVHPCYFLWHRYSCYVGGWEKQIKPWSFGNLKERNILD
                     IWNDRNYRSFRESVLRYDFPFCFDCSFALCDYVQGEDFEQDCYVSSVPCGACLWCTGL
                     FHCLQ"
     gene            9193..10929
                     /locus_tag="VMU21_04270"
     CDS             9193..10929
                     /locus_tag="VMU21_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HUO76774.1"
                     /translation="MDKVLRINVGAAGGPKATEVPVGEYAGFGGRAMTSAIVSKEVPP
                     LCHPLSGENKLVIAPGLLSGTAGAQSGRISVGGKSPLTGGIKESNSGGQASQVLAKLG
                     YNAIVLEGKPANDDLYKVFINKDGVKITKDNSLKMLPNYDLIAKMKAEFGDKVACISI
                     GPAGEMKMGAATVAFTDMEQRPTRHAGRGGMGAVMGAKNIKVIVLDATGMPVREPKDP
                     QKFMDANKRFVDGLKKHPVTGQGLPAFGTNVLTNILNEVGGYPTYNFKQGRFDGASKI
                     SGETQAETEKKRGANPTHGCHRGCVIQCSGTYNDKDGHYLTKQPEYETVWAHGGNCGI
                     GDLDAIAKLDFLDDNYGLDTIEMGATIGVAMEAGLAKFGDAQAAIDLVNEVGKGTYLG
                     RILGNGAGMTGKAFGVERVPVVKNQAMPAYDPRAVQGIGVTYATSPMGADHTAGYAVA
                     QNVLKVGGDVNALKPEGQVELSRNLQIATAAIDSTGMCLFIAFAVLDQPDTFQALLDM
                     LSAFYGINMTGDGVTDLGKKVLKMERDFNKAAGFTPQQDRLPSYFKTEQFAPHNVTFQ
                     VKDEDLDKVFNW"
     gene            11145..>11520
                     /locus_tag="VMU21_04275"
     CDS             11145..>11520
                     /locus_tag="VMU21_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76775.1"
                     /translation="MVKALVGCSIKSFLTQQMGISPEYIAGRIHSIFLEGRPVDDIDS
                     AMIHDGSHLSLSAAMPGLVGATMRRGGVYGAFRSSITYNESDSHCAVAEGCVHLKFFN
                     LLMGELGPDLLKKGIYLKSSQMA"
BASE COUNT         3063 a         2861 c         2979 g         2617 t
ORIGIN      
        1 accaaaccat aagcatcctc cctgataaaa ttggcctcgc cacgtgtcat cctcttcttc
       61 ctcgttatca taatcccgct cctcttgctc tccatctacc ccacagccgg ccctccttca
      121 aaaaactggg ggatggcaag ctcgtacaag tacttgacag cttctggttc taggtctata
      181 attcatttta atcccagtta tgaggtgagt tcatagctct ctggattgat ataattatta
      241 tttacgaaaa tttcctggga gaaaagagtc ctataatcca aacagtgctg aacgtactat
      301 catttgaccg cagactttat tattccttaa atttgtaaat atccacataa acctgttctc
      361 ggttgatatc ttaaagcgat cttcaaccgg gtgaggaggg ggggcaatat gaacgtatct
      421 cgccgcaact tcctgaaagt ctcaggagga accatgttac tcgggtctct ggggataaac
      481 ctggaccctg tcaaggctta cgctcagacg ttgcgcatta aggatgcaaa ggagtcttac
      541 accatctgtc catactgctc tgtaggctgc gggattaccg cctatgtgaa ggatgggaaa
      601 atcatcaata ccgaaggtga ccccgagcat ccaatcaatg aaggcacgct ctgtccgaaa
      661 ggggcatcgc tctaccagat ggcgaacaat cccctgagag tgacaaaacc tctttaccgt
      721 gccgcgggtg caaaggaatg gaaagaggtg gaatgggact gggcccttga ccagattgcg
      781 cgaaaggtca aagacacaag ggacaggacg ttcagggaaa gagcgaaatc aaaagtaaag
      841 gaaaagaaga ctgaacccgg cacaaaagag ggcggtctgc ccgttgaggt ggagatcgat
      901 gtcgagaagg aattcgtggt gaacaggacc gacggcatcg cccatgtggg gagtgctgct
      961 ttggacaacg aagagtgcta tctgctccaa aagatgctcc gctcctgggg cgttgtctat
     1021 atagaacatc aggcccgaat atgacactcg cccactgtag cggctctggg agagtcgttc
     1081 ggtcgtggcg caatgacaaa ccactgggtt gatctcagaa acgcggacgt catcctcgtc
     1141 atgggttcaa accctgcctc taatcaccct atagccatga agtgggtcat gaaggcaaag
     1201 gagaagggcg caaaactcat cgttgttgat ccccgcttta cacaaactgc ggcaaaggct
     1261 gatatctatg cccctctccg ctcgggaaca gacatagcct ttatcaacgg gatgattaag
     1321 tacattatcg ataacaacct tatcttcagg gactatgtgg tcaactatac caatgcaact
     1381 tgcctggtaa atccggactt caagctgccg gtagagttgg atggcgtctt ttctgggttt
     1441 gatgaaaaga ccgcaaagta tgacaaaaag acctggacat tccagatgga cgagaaaggt
     1501 ctccccaaga gggacctgac cctgaaggat ccccgctgcg tatatcagat cctgaggaag
     1561 catgtttccc gttatacccc cgaaatggtc tccaacatta ccggaacacc aaaggaaaag
     1621 ctcattgagg tatataagac ctttgcatcc acgggaaagc gggacagggc tggaaccgag
     1681 tgttatgcaa tgggatggac ccagcatact gtaggtgtgc agaatatcag ggccttctgc
     1741 attgtccagc tcctcctcgg gaatatgggt attgcaggag gtggaataaa cgcaatgaga
     1801 ggggaatcaa atgtccaggg ctcgactgac tatggtctcc tcttccatat cctcacaggc
     1861 tataacccca cgccctcagc ttcacttgtc gaccttaagg cctacaatga aaagttcacg
     1921 ccgaaaacaa aggagccgaa gagcgtgaac tggtggggaa acagaccgaa atacatgaca
     1981 agctacctca aagcgattta tggagacaag gcgaccaagg acaatgatta tggatatggc
     2041 tggcttccca agcttgacga ggggatgaac gcctcatggc ttagactttt cctcgccatg
     2101 ggcatgggga acttcgaagg tttctttgcc tgggggcaga accctgcctg ttcgtccgct
     2161 gctgcggcaa aggtgagaca ggttttcaca aagctgaaat ggctcgtgaa tgtgaacctc
     2221 tacgacaatg agaccgcatc cttctggaaa gggcctgaca tgaaaccgga ggagatccag
     2281 acagaggtgt tcatgctccc ttgctgctcc tctgtggaaa aagagggaag catcagcaat
     2341 tccagccgcc ttgcccagtg gagaacagcg gccattaagc ccatcggtca gtccatgcca
     2401 gactcagaga tcatgaacga gctgtactac agggtgaaga agctctacca gaaagagaaa
     2461 gggaagttct ctgcgccgat ccttaacctg acctgggatt acggtgaaaa gaacaaggat
     2521 gggaagatag agcattttga tatccaccgt gtggcaaaag agatcaacgg atactacctt
     2581 gaagatatct atgacaagaa ggttgacccg cccaagctca ttggaaagaa gggacaactt
     2641 gtcacaaact ttgtgaccct ccaggcagat ggcaccacat catgcggcaa ctggttatac
     2701 tccggaagtt acacccagaa agacgataag gtcataaaca tgatggccag gaggggcaag
     2761 gaagacccca caggccttgg actctatccc aactgggcgt tcgcctggcc gttgaacaga
     2821 aggatcatct ataacagggc gtcggttgac cctaacggga atccctggga tcccaagcgg
     2881 gctgtcctta aatggaaccc caccaaggtg aaccctgcca caaataaacc tggtgtctgg
     2941 gaaggtgatg taccggacgg tcctgctcca ccgttatcag atgaaaaggg tggaaagctg
     3001 cctttcatta tgaagcctga tggaatcgct tccattttcg ggccaggcct tgctgacggg
     3061 ccattccctg agcactacga acctctggaa tgccccattc aggaaaaccc catgtccaag
     3121 aagcggagga taaatcctac cgcacacccg attgcgcaga tggccgcgca ttttagctgt
     3181 gatgtccgct atccttttgt tgcctcaacc tatagggtaa cggaacactg gcaaacgggt
     3241 gtcatgacaa gacatactcc atggcttctt gagatgcagc cccagatgtt tgtggagatg
     3301 agcaaggagc tcgctgcgga aaaagggatc aggaatggcg acaaggtaaa ggtctcatca
     3361 gcacgcggaa gcctttcggc cgttgcagtg gtcaccgaac ggttaaaacc gttcaaagtc
     3421 atgaaccaga cgatccatca ggtcggtatg ccgtggcatt tcggttggca gttccctgtg
     3481 gacggaagcg ggggggacag cgccaatctt ttgactccgt ttatcggaga tccgaataca
     3541 agcatacctg aatcaaaggc cttcatggtc aatgtggaaa aggcataggg ggtgatatta
     3601 tgacaagaat gggaatactc gtctcaccgg aattgtgtat aggatgcagg gcctgtcagg
     3661 ttgcctgcaa gtcatggaac cagcttcctg gcgacaagac agtgaacaga ggaggctttg
     3721 agaaccctcc cgatctcacc ccgcaccttt acaacaagat acactatctt gaggtgcccg
     3781 cagccacaga cgttcagcgc tggctcttcg tgagtcaacg gtgcatgcac tgtgacgatg
     3841 cgggctgcat gaagatctgt ccttccccgg gagcccttta taagacaaaa gaaggcgctg
     3901 tcgcgatcaa taaggacaaa tgcatcggct gcaaactctg cgtcgccgga tgtccgttca
     3961 atgttccacg ctacgatgag aaagacaaga tttcaaaatg ccatctctgc tacgacagga
     4021 tcgcaaacgg actccaacct gcatgtacaa aaacctgtcc tacaggtgcc ctgaaatatg
     4081 gtgaaaggga cgcccttatc agcggcgcgc aaaaagccgg ttatgcaaag ctctatggac
     4141 agacagacct gggaggtctt ggatcgctct acgcgttcaa ggatgcccct aaggtctacg
     4201 gaatggatga aaggccggcc atacccgaga ccattgtctt ctggcataaa gtgctcaaac
     4261 ccttgtcgta tatagggctt ggcggcgtcg tggctgcgtc cctgatccac tacgttgcca
     4321 ttggtcctca caaagagaag gaagaggagg aaaagaaaaa tggatagaat gatcaagaag
     4381 gcaggcgcat tcgaaatcat caaccactgg gtgatggcca ttagctgtat tctcctcgcc
     4441 gtgcagggat atgggttcct tttccacgtc gagggagttg gctcggtgtt tggcggattt
     4501 aactcaatgc ggaacctcca taactacctt ggcatcgcct tttcgatctc tctctttttc
     4561 tccatcttta tgtatctcaa ggaggccctc acctttgatg ctgatgatat tgggtggata
     4621 atggtactgg gaggttatct ttcccataag gtaactgttc cgcccatggg gaagatcaat
     4681 accggccaga agttcttcta tcttgcagtg ctcatctttg ggatcggtat ttccgcatca
     4741 ggctatgtca tatggcttct cccgggcaat aagcagtata tcctcctttc ccacattgtt
     4801 cacaatatca gttttgtcgt tttgatgatc gccatacccg ctcacatcta cctcggtaca
     4861 cttgcgaacc ccgggaccct gaggataatg ctttccggaa ccgttcccta ccaatgggcc
     4921 aagaagaggc atccaaagtg ggtagctgag gtggagggag ggcataaagc ttgagtattt
     4981 ttgctcctct atatatggaa aaggttcttg gatagtctct aaggaatccc ccatgccact
     5041 agaaacctaa gtgacatggg ggattatcaa aaaggaaaac catgaagaaa ttgttaattc
     5101 ttgttgttct gtttttagca tcatccctct cctctgcaca ggataaacga tatgaagttc
     5161 ctgtcgggga ttcaccgggg tatggtcagg ccaatgcccc ggtgaccatg atcgagtttg
     5221 tcgattacca gtgaccgcac tgcgtggggg tcggtccgac tgtcgataaa ctactcaaag
     5281 actacaaagg gaagataagg cttgtggtga aaatgaaccc ttacaagtat cgggacttct
     5341 cccacatagc cgccgaggcg gtacttgcag cccgtgacca gggaaaattc tgggaaatgc
     5401 atcaactcct gttgaagaaa tcaccgcagc ttgacagaac cagtctcata aagtatgcac
     5461 aagaaagcgg cctcgacgtg aagaaattta ctgaatccct tgatacaaaa aagaacgcga
     5521 gtgccattga acgggacaaa caacttgcca tttcccttga tctgtacgag acacctgtat
     5581 tttttattaa tgggagaaag gtcacgggaa acagggccta tgactatttc aaaaagatca
     5641 ttaatgagga aatgaagaat gagaaaaggt agactttcct ttttgccctc ctttgcggtc
     5701 atgtggggta ttattttatt cgtcctcact cttgatgtac acgctgggca ccttgagcct
     5761 tttaaattcg ttccgattcc gcaacacaat ccccagactc ctgaaaagat tgacctcggc
     5821 aagaaactct tttttgacag gcgtctttcc ggagacggca ccatgagttg cgccagctgt
     5881 cacatacccg aactggcgtt caccgacgga caggatattt ccctcagcta tcccacaaca
     5941 aagaactgga gaaactctcc caccctcatc aatgcggcat tttataaatt cctttttcat
     6001 gacggccgcg tcaggacctt ggaagaccag gcacttttcc ccatgatgtc agcgtttgag
     6061 atgaacaaga atctcgacta cctcgaggag caaatcaggg tggtaccaga atatgctgaa
     6121 gccttcaaaa aggtcttcgg gggtgaagtc acgaggaaga ggattgcaat ggcaatcgca
     6181 tcgttcgaga ggaccttggt ctctttgaac tcaccacttg atcggtacct gaaaggggag
     6241 aaaggtgcgc tctcagaagg tgcgaagaag ggatttgaga tattcaaagg caagggaaag
     6301 tgtgctgatt gccatcatgg ggtgaacctc tctgacgaca agttctatgc gcttgaagtt
     6361 ccggaaaatc ctgttctgtt gaatgatccg agggttaccg ccacgatgcg atttgtggca
     6421 aaggtatacc attatgacga ttacaagaac ctgacagagg atcccgggag gtatcttatt
     6481 acaaaaaaca aaaaagactg gaaagccttc aggactccca cattaaggga aatttcaaaa
     6541 accgctccct acatgcacaa tggtgtattc aagacgcttg acgagatcat tgatttcttc
     6601 gactccggag gagggaaggg aaatagagcg ctgaaacctc tgggcctgag cgccgaggat
     6661 aagacggacc tgaagtcatt tctcgttgag gcgctgaatg gtgaagagat acccatacaa
     6721 tatcccgaaa tcccgtaagg gtaatacccg aacattgaaa tgaacattga agaaattata
     6781 caggaaaaac cccatctcaa agagaccatc ctgctctatg aaaaggtttt gacttttgac
     6841 agcatggtgg caaaaatgac caatgtcatt accttggatg aggtgactta tcctcctgaa
     6901 atagcggatg cggtggtgga acgcttttca tcaaacttcg gcatcccgga agaacatctg
     6961 cttcctttca aggaagcact gaaaatgggg cagcttgatt tcacaagact tcccctaaac
     7021 gagattccct ctttttcctt tccctatcac gaagatgagc ttggattaat tctcttcctc
     7081 atgagcaagc ctttcttcct tcggctggga gaaatgtatc atctcagcgg gagcacatgg
     7141 gaagagggca ggtgtccggt ctgccatgca aagccttccc tggcatccat cgacagtgaa
     7201 aacaggagaa aactgtattg ttcattctgt ggcactaagg gatattacac acgccacggc
     7261 tgcccgctct gctcaaatga ggatacttca aagatcactc tttttacctt tgaccaggag
     7321 aaggatttca ggattgacgc ctgccaccag tgcaaatctt acgtcaagac agtcctcaat
     7381 gagagcatta tcgagaaccg cctcaccccg gacctcgctg acctcatgag ccttcccctt
     7441 gacgtcatcg cccaagggaa gggcttccaa cgccattcac ccaaccccat tggcatgacg
     7501 aagatgggat aaaaggccaa tacaggacta tttcacagtg tatgacgtac ctcatattct
     7561 tcttcccctg ccctgctaaa atataagaga gattcgaaga ccctccctgt aaagggaagg
     7621 gtgaggagct ataattgttt ttaagagatg atgagcccag gggtagccac tccaatgaat
     7681 tagaggaaat acaaaactct tccctgaaat caagaacgta tccctcaaaa ctttttgttg
     7741 aggtcaccac aaggtgcaac ctcagatgtg ccatgtgtgt aaagcagaac acctccactg
     7801 gcatgtcaga gggaaacatc tcccttgaga cgtttcgtgc gctggaacct tcattccccc
     7861 acctttctgc cctcattctt aacggcgtag gagagcccct gctctatcca caattagaag
     7921 agttcatacg cagggcaaaa aaacaattgc cgcacaatgc atgggtaggt tttcagacaa
     7981 acggagttct tttaaacagg gaacgcgctc tgtccctcgt tgaagcagga ctgggccgta
     8041 tctgcctttc catggatgcc atttcccgcg acacctttcg caggatccgt gagggaggtg
     8101 aggtgagaga cctggaatcc gcattctctg ccttgaacgc tgcagtaaag agtgcaaatg
     8161 gtcacacgag gcggcgtctc gacatcggca tcgagttcgt actcatgcgt gaaaacctct
     8221 ttgaactccc ccaaacggtg agatgggctg cacaccgcag ggccacgttc gcccttgtca
     8281 cacaactcct tccctgcacc aaattaggga ctaaccaggc tgtctacgac accaataccc
     8341 atggggccat atctgtgtat gacctctgga agaacagggc aggacttcaa gggttggata
     8401 ttcggaggta tttcgatgtg ttcatgaaac actttaagaa tgctgacgaa gaaaacattt
     8461 ttgctctcat cgaagaaatg aaggccgacg ctggttctcg gggaataacc ctccatctgg
     8521 aacggctcct cctgagggat gaagaatggt tgaaaagggt ggaagagatt ttcgaagaga
     8581 ccttagagat tggacaagag gaagggattg aggtaaagct ccctgaaatt gccccaagga
     8641 acaatagaaa gtgcgagttc gtcgaaggag agagctcgtt tgtatcatgg gagggagacg
     8701 tgcatccatg ctatttcctg tggcaccggt atagctgtta tgtgggggga tgggaaaaac
     8761 aaatcaagcc ctggagtttc ggtaatctga aggaaagaaa catccttgat atctggaatg
     8821 acagaaatta cagatccttc agggagagtg tccttcgcta tgacttcccc ttctgttttg
     8881 attgtagttt tgccctctgc gactatgtcc agggtgagga ctttgagcag gattgttacg
     8941 taagcagcgt tccctgcggg gcatgcctct ggtgcaccgg tctgttccat tgcctccagt
     9001 aagattttcg tctcctccaa tcaatatctc ctttcacgtt tcccctctca catgaaaata
     9061 atttatttga caaatgaatt gttttcacct atcctcttat aaagaagtat caagttctat
     9121 ccgcattgca aatgttaacg tttcatcaaa catatccctg ttttatgcag aagggaagaa
     9181 tgggaggatt gtatggacaa ggttctcaga attaacgtag gggcggcagg tggaccaaag
     9241 gctactgaag tccctgtggg tgaatacgcc ggatttgggg gcagggcaat gacctctgcc
     9301 atagtttcga aggaagttcc gcccttgtgc catccgttga gcggcgagaa caagctggtc
     9361 atcgcgccgg ggctgctgag cgggacagcc ggcgctcagt cgggacggat atctgtgggc
     9421 ggtaagagcc ccctcaccgg cggcatcaag gaatcgaact ccggaggaca ggcctcccag
     9481 gtcctcgcaa agctcggata caacgccatc gtgctcgaag ggaagccggc gaatgatgac
     9541 ctctacaagg tcttcatcaa caaggacggg gtcaagatca ccaaggacaa tagcctcaag
     9601 atgctcccga actacgacct gatcgcgaag atgaaagcag aattcgggga caaggtggca
     9661 tgcatttcga tcggacctgc aggcgaaatg aagatgggcg ccgcgacagt ggcctttacc
     9721 gacatggagc agcgtccgac ccgtcacgca ggccgcggag ggatgggcgc ggtcatgggc
     9781 gcaaagaata tcaaggtcat tgttctcgac gccaccggca tgccggtgcg cgagcccaaa
     9841 gacccgcaga agttcatgga cgcgaacaaa cgtttcgtag acggcctcaa gaaacatccg
     9901 gtcaccggcc agggccttcc ggcttttggt acgaacgtgc tgacgaacat cctgaacgaa
     9961 gtgggcggct atcccaccta caactttaag cagggccgtt tcgacggcgc ctccaagatc
    10021 agcggcgaga cccaggcgga aaccgagaag aagcgaggcg cgaacccgac ccacggctgc
    10081 caccggggat gcgtcattca gtgttcgggc acctacaacg acaaggacgg ccactacctt
    10141 accaagcaac cggagtacga gaccgtctgg gcccatggcg ggaactgcgg gatcggtgac
    10201 ctggatgcca ttgccaagct ggactttctg gatgacaatt acggtttgga caccatcgag
    10261 atgggcgcga ccattggggt agccatggaa gcgggcctgg caaagttcgg agatgcccaa
    10321 gccgccattg accttgtaaa tgaagtgggc aaaggcacat acctcggacg catccttggt
    10381 aatggggcag gcatgaccgg caaggccttc ggcgtcgagc gggtgccggt ggtgaagaac
    10441 caggcaatgc cagcctacga tccccgcgcg gtgcaaggca tcggcgtcac ctacgctacg
    10501 agccccatgg gcgcggacca caccgcggga tacgcggttg cccaaaacgt cctgaaggtg
    10561 ggcggcgacg tgaacgcgct caagcccgag gggcaggtcg aattgtcgcg gaacctccag
    10621 atcgcgactg cggcgattga ttccaccggc atgtgcctct ttattgcctt tgcggtgctc
    10681 gaccagcccg ataccttcca ggccctgctc gacatgctca gcgcgttcta cgggatcaac
    10741 atgacgggcg acggcgtgac cgacctgggg aagaaagtcc tgaagatgga gcgggacttc
    10801 aacaaggctg cgggcttcac ccctcagcag gaccggctgc cgagctactt caaaactgaa
    10861 cagtttgctc cccacaatgt cacattccaa gtcaaagacg aagatctcga taaggtcttt
    10921 aactggtaat tgaataaaag aaaccctttt ggagggagag gctgaaagaa agcctctccc
    10981 ttttttgtcc ataacacaaa gggaccgaat acgtaggcag aggtatcgtt tctatgacct
    11041 gttttaggcg acaagatttt gataatagtt ccctcacaca gctctgtttt gtaatagaca
    11101 atgctcagct tcccggtttc taccaaatcc tccaggctgg gtgcatggtc aaggcgcttg
    11161 tcggctgcag catcaagagc ttcctcactc aacaaatggg aatatctccc gaatatattg
    11221 cagggcggat acactctatc tttcttgagg gaaggcctgt ggacgacatc gattccgcaa
    11281 tgatccatga tgggtcacac ctctccctct ctgctgccat gccgggtttg gtgggggcca
    11341 ccatgaggcg tgggggcgtc tacggggcat ttcgaagctc cattacgtat aacgaatctg
    11401 attcccattg tgcagttgca gaaggatgtg tgcacctgaa gttctttaac ctcctgatgg
    11461 gggaattggg tcctgatcta ctcaagaagg gtatctatct gaaatcttcc cagatggcgg
//
LOCUS       DATKOU010000093         8983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_889200, whole genome shotgun sequence.
ACCESSION   DATKOU010000093 DATKOU010000000
VERSION     DATKOU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889200"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1257
                     /locus_tag="VMU21_04280"
     CDS             175..1257
                     /locus_tag="VMU21_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HUO76776.1"
                     /translation="MQEKLFMKGNEVIAEAAIHAGCRFYAGYPITPQNEIPEYMSRRM
                     PEVEGVFIQAESELAAINMVYGASACGARAMTSSSSPGVSLKQEGISFLVGSELPAVI
                     VNVQRGGPGLGNISASQADYFQAVKGGGHGDHKLLVYAPCNLQELWDLTILAFDKSDE
                     YRNPAMILTDGILGQMMEPFYPTPYHRTQIPEKTWALTGCLGRKPNVLKSLYMGEGEL
                     EMRNNLLQEKYRRMKEKEVRFEATAVEDAELVVVAFGIAARIALSAVRCLRNEGIRVG
                     LFRPISLFPFPEKELSGLAGNGRDFLTIELNAGQMVEDVRLSVNGRSEVHFYGRPGGA
                     IMTPEEVQEKIQSLLTSHYSTSQTRS"
     gene            complement(1220..1771)
                     /locus_tag="VMU21_04285"
     CDS             complement(1220..1771)
                     /locus_tag="VMU21_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HUO76777.1"
                     /translation="MKIIIEKAIKNKTLRLVQGDITERNVDAIVNAANSHLQHGGGVA
                     GAIVRKGGSVIQEESNRVGFVPVGNAAITGAGKLTARYVIHAVGPRMGEGDEDNKLRR
                     AVLSSLSLASGKGLKSISLPAISSGIFGFPKDRCAEILVATASRYIGENPGTSLELIE
                     FCVYDDLTTKYFRTEFEKLSSER"
     gene            2131..3639
                     /gene="hflX"
                     /locus_tag="VMU21_04290"
     CDS             2131..3639
                     /gene="hflX"
                     /locus_tag="VMU21_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HUO76778.1"
                     /translation="MSTEIGRQVGILVPRSGTITHVIVGDTKGILIPDLADYPIGRKA
                     LRGLRLIHTHLVDEPLTHDDLTDLSLLRLDLIAAIGIKDGLPDRIFVAHLMPQGSEKP
                     VEVFPGQGFHQFDMDFLSFVGALEDEMERRRTFDQQDGRERAILVSVSTRPKYEQEDT
                     MEELRELALSSGVLVLDGIIQRPKEVNPKYLMGEGRLKELIISAMNKGATVLIFDQDL
                     SPSQTKAIGELTELKVIDRCQLILDIFAQRAHSRDGKVQVELAQLKYRLPRLTGKGTA
                     MSRLMGGIGGRGPGEMKLEIDRRRVRDRIHLLARELRNLSEARRQRKQRRVEKGVPIV
                     SIIGYTNAGKSTLLNALTKSDTCVEDKMFATLDTASRRLRFPREREAIVTDTVGFIRN
                     LPRDLMAAFRSTLEELEDADLLVHLVDASSPRFEQHITSVDKILEELHLRNKPQLLVF
                     NKIDKITNEEADNLRIRFHAITVSALDSSTFISLLEAIESYIWNSKMADATV"
     gene            3741..4817
                     /locus_tag="VMU21_04295"
     CDS             3741..4817
                     /locus_tag="VMU21_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76779.1"
                     /translation="MEFKPKWIAWEITRRCNLRCVHCRSSSELEAKGHPDFSREDAFR
                     ILENIAGYAKPVVVLSGGEPLIREDVFDIARYGTEKGLRMCLATNGTLVNDTICEKIK
                     DSGIRIVSLSLDGSTDAIHDDFRNQTGAFSGTVQAARLFKKHGIEFIINSSFTKRNQE
                     DIPNVYRLAKELGASAWYMFMIVPTGRGEEIMNELISKEDYEDILEWHYQMEKDEHDM
                     LVRPTCAPHYYRVVLQKAKEEKVKFERRTLKFSTGGSKGCIAGQLICLINVDGDVFPC
                     SYFPKSAGNVKVQSFQEIWEDSELFKELRDFKKYKGRCGSCEYINVCGGCRARAYSLH
                     GDFLEEEPFCNYMPVKMQKKTIRG"
     gene            4833..5855
                     /gene="hemE"
                     /locus_tag="VMU21_04300"
     CDS             4833..5855
                     /gene="hemE"
                     /locus_tag="VMU21_04300"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HUO76780.1"
                     /translation="MNDTFLKACRGEEVPYTPVWVMRQAGRYLPEYQAVRAEVDFLTL
                     CKTPELAAKVTMQPIQRFGVDAAILFSDILIAVEAMGMHLEFSDKKGPVLGDPLRTKS
                     GVDRLIVPDTEDSMPFVIEAIKILRSQLKVPLIGFSGAPFTLATYMIEGGSSKNFLNT
                     KKMMFQNPGLFSLLMDKITMTVVSYLSAQIRAGAQAVQLFDTWAGILAPEDYKEYVLP
                     SVKKAVAELKKEGVPVIYFVNECAALLKELKKTGADIIGIDWRIDLKDAVKGLGKKFV
                     VQGNLDPCALFLPREKMEDKVKDILWKGEFAKGHIFNLGHGVLPESPVENVAALVEAV
                     HRFSER"
     gene            5809..6852
                     /locus_tag="VMU21_04305"
     CDS             5809..6852
                     /locus_tag="VMU21_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO76781.1"
                     /translation="MSLRWWRLSIDLVNGSLTKRRLISNAARIALVYFLASFVYIYFS
                     DRLVEVFIQDFNLLTSVQTFKGAAFVALSSLLIYVLVIRKERENERLLRELDSKVKER
                     TTKLEEAMLFAESTSKLKSEFLANMSHELRTPLNAIIGFSEAMLSGVYGPVNERHREY
                     LGDILTSGENLLGLINDILDLSKIEAGSMDLERRTFSLKELMKSAVGMFREKSVAHNI
                     TLECHVEDGVDEIVADQRKVKQVMVNLLSNALKFTPAGGTVRMNARKAADTIEVSIKD
                     TGIGISSHDIPHLFQPFHQLESPFQKKYSGAGLGLFLTKRLVELQGGGIRVESEKGEG
                     SVFSFSIPIKGNV"
     gene            6929..8026
                     /locus_tag="VMU21_04310"
     CDS             6929..8026
                     /locus_tag="VMU21_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO76782.1"
                     /translation="MDDDELNLRFMSSMLEAQGYQVEIARNGAEGIEKVLSFHPDVTL
                     LDIMMPEMDGYEACLRLKNREETNNIPLILVTALDDRESRLKGFKAGADEFLTKPIDR
                     VELTLRLQNLIKVKEYGDFLKDYSKTLESMVNQRTLELENAYREVENANERIKKGYLD
                     TIYRLTMAAEYRDEDTGNHLRRISFYSQMIARELGMSDAFVDTICYSSPMHDIGKIGI
                     PDAILLKADRLTCEEFELAKTHTIIGGNLLKGSESEILQMAERIALNHHERWDRTGYP
                     SGLGGNGIPIEGRIVTMADQYDALRSRRPYKPWMSHEEAFAVITKGDGRTRPEGFDPE
                     VLEIFIKREEDFRMIYEEHKDYRSDQLTDSG"
     gene            8162..>8983
                     /gene="hemH"
                     /locus_tag="VMU21_04315"
     CDS             8162..>8983
                     /gene="hemH"
                     /locus_tag="VMU21_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HUO76783.1"
                     /translation="MLLLNLGGPDSLQAVKPFLYNLFSDREIIRLGPAIFQKPLARLI
                     STFRRKKTEKMYSLIGGRSPILEITLAQADSLEKALNQKPALTSHQSLNKEAESIHNL
                     QEPPQRRLFEVFVGMRYWNPFIEDAVRKIYGEGIRAVLAVTLYPHYSLATTGSAVKKF
                     NEIIRQYPMEASCISSWYNHPYYIDALVERIRQGVLSFQGEEAEVLFSAHSLPVSIVE
                     SGDPYVDHIMETIGEVVKETDLRWHLSYQSKSGPVKWLEPSTEDALRELAKRGVRN"
BASE COUNT         2413 a         2024 c         2420 g         2126 t
ORIGIN      
        1 catgtcatat ggctgaagga gcacgcattc cttctttgac caaaattcat ggagcttcag
       61 tatgagagtt tgaaagttca tggcgatgac aagaaatcat aaaggaagcg gaagatgttt
      121 gtcaacgaaa gtacgggggc attgagcctt atcgatgtgg tataattgca tacaatgcag
      181 gagaagctgt tcatgaaagg caatgaggta attgccgagg cggcaattca tgccggctgc
      241 cgtttctacg caggatatcc catcactccc cagaatgaaa ttcccgaata tatgtcccgg
      301 aggatgcccg aggttgaggg ggtgttcatt caggcggaga gcgaactggc agccataaac
      361 atggtgtacg gggcctcagc atgcggggca agggcaatga cctcttccag cagcccgggg
      421 gtgagcctta aacaggaggg aatatccttt ctcgttggtt cggaactccc cgcggttatt
      481 gtgaatgtgc agaggggcgg tcccggactt gggaatattt cagcaagtca ggcagactat
      541 tttcaggcgg tgaaaggggg aggtcacggc gatcataaac tccttgtgta tgcgccctgc
      601 aatctccagg agctctggga tcttaccata ctcgcctttg ataaatctga tgaatacagg
      661 aaccctgcca tgattctcac tgatgggata ctcggccaga tgatggagcc gttttaccca
      721 actccttacc atcggaccca gatccctgaa aaaacatggg cgctcacagg atgtcttggg
      781 agaaagccca atgtgttgaa atccctttat atgggtgagg gtgaactgga gatgcggaac
      841 aatctccttc aggagaagta tagaagaatg aaagagaagg aggtgaggtt tgaggcaacg
      901 gcagttgagg atgcagagct tgtcgtggtt gcctttggca tcgctgccag gatcgccctt
      961 tccgcggtaa gatgcctgag aaacgaaggt atcagggtag ggctcttcag acccattagc
     1021 cttttcccct ttcctgaaaa agagctctcc ggacttgcag gtaacggaag ggactttctt
     1081 actatcgaac tgaacgccgg tcagatggtg gaggatgtaa ggctctccgt gaatgggaga
     1141 tcagaagtgc atttttacgg gagaccaggg ggggcaatta tgacccctga agaggtacag
     1201 gaaaaaatac aatccctcct tacctctcac tactcaactt ctcaaactcg gtcctgaaat
     1261 attttgtggt gagatcatca tatacgcaga actctatgag ttcgaggctt gttccagggt
     1321 tttctcctat gtagcgtgat gcagtggcaa ccaaaatctc tgcacacctg tccttgggga
     1381 atccgaagat ccccgaactt atcgctggca gagaaatgct cttcaacccc tttcctgatg
     1441 cgagactaag gctgctgagg accgcccttc tcagtttatt gtcctcgtcc ccttctccca
     1501 tccgcggtcc cacggcatgg atcacatatc tggcagtaag ctttcccgct ccggtgatag
     1561 cggcattgcc taccgggaca aagccgaccc tgttactttc ttcctgtatg acagagccac
     1621 cctttctgac tatagctccc gcgacacccc ccccatgctg caaatgagag ttggcagcat
     1681 tcactatcgc atccacattt ctttcggtaa tatccccctg gacaaggcgc agggttttgt
     1741 tttttattgc cttctctatg attatcttca tgggttcccc cttccctagt ttaggggcaa
     1801 tagtcgtctt tcactctttc ccagatgaaa cacctctctt acaaatcttg gggtcgatga
     1861 gccataactc aacatacttt ttttccaaag ttataataag gcactttgcc tttttataac
     1921 tgtctctcac gtctgttcat acccattata taaaacttca gtctgtgata aaataccata
     1981 aagacctagg gaggattcag aataccaacc atctacggca acatctcggg ccttaaaggg
     2041 acacagatcc gctcccttga aaggatctat cggagaaaga tccctcagga taaggtgatc
     2101 acccctgaac ttgctcggta tttaggagag gtgtcgacag aaataggccg tcaggtgggg
     2161 atattagttc ccaggtcagg cactattacc catgtaattg tgggagatac aaagggaatt
     2221 cttatccctg accttgccga ttatcctatt gggagaaagg ctctacgagg actccgtctc
     2281 atccataccc atctcgtgga tgaacctctt acccatgatg atctcactga cctttctttg
     2341 ctgagattgg atctcattgc agcaatcggt ataaaggatg gtctccctga ccgcattttt
     2401 gttgcccatc tcatgcccca gggctctgaa aaacctgtgg aggtatttcc tggccagggg
     2461 ttccaccaat ttgatatgga tttcctttcc tttgtcgggg cccttgagga cgagatggag
     2521 agacggcgca cttttgacca gcaggatggc agggagaggg ccatcctcgt gagtgtatcc
     2581 acaaggccga aatacgaaca agaggatacc atggaagaac tcagggagct ggccctctcc
     2641 agcggtgtcc ttgtcctcga tggaatcatc cagaggccta aggaggtaaa tcccaagtat
     2701 ctgatgggcg aaggaaggtt aaaggaactc atcatcagcg caatgaataa gggtgcaact
     2761 gtcctgatat ttgaccagga cctctcgcca tcacagacca aggccatcgg agaattgacc
     2821 gagttgaagg tgatagaccg ctgccaattg attctggaca tattcgctca gcgggcccac
     2881 agcagggacg gaaaggttca ggttgaactt gcacagttaa aataccgact gccgaggtta
     2941 acgggaaaag ggaccgccat gtcacggctg atgggaggaa tcgggggaag gggaccgggt
     3001 gaaatgaaac tggaaattga caggaggcgt gtgcgtgaca ggattcacct gctggcaagg
     3061 gagctaagaa atctctcgga ggcaagaaga cagcggaaac agagaagggt agaaaagggt
     3121 gtccctattg tgtccattat cggctataca aatgcaggga aatcaaccct cctgaacgcc
     3181 cttaccaaga gcgacacgtg tgttgaggat aagatgttcg cgaccctcga cactgcgagc
     3241 aggaggctca ggttcccccg ggaacgggag gccattgtta ccgacaccgt ggggtttata
     3301 agaaatctcc caagggatct catggcagcc ttcagatcga cccttgagga gctcgaagat
     3361 gcggatctcc tcgtacatct tgtggatgcg tcaagcccga ggttcgagca acatatcacc
     3421 tctgtggata aaatacttga agaactccat ctccggaata agccgcaact tttggtgttc
     3481 aacaagattg acaagatcac gaatgaggaa gcggacaatt taaggatacg gttccatgcc
     3541 atcacggtct ctgccctcga ttcctcaaca ttcatatccc tcctggaggc aatagagagc
     3601 tatatttgga atagcaaaat ggcagatgca acggtataat agatgtgcgt gcaatacgca
     3661 acgggtcatt aaatttaaga aggataagcc gattaggaaa accgagtgcc ggttttctcc
     3721 ttcggaacac aggagtatac atggaattta agccgaaatg gatagcatgg gaaattacgc
     3781 ggagatgtaa tctcaggtgc gtacattgcc ggtcttcttc ggaactggag gctaagggcc
     3841 atcctgattt ttccagggaa gatgcattca ggatactgga gaacatagcc ggttatgcaa
     3901 agcctgtagt ggtgttatca ggtggtgagc ccctcataag ggaagatgta tttgatattg
     3961 cccgctatgg cacagagaaa gggctgagaa tgtgcctggc gaccaacgga accctcgtca
     4021 acgatacaat atgtgaaaag ataaaggatt cggggataag gattgtatcg ttaagcctcg
     4081 acgggtcgac agatgctatt catgacgact ttagaaatca gacaggggca ttctccggga
     4141 ctgtgcaggc ggcgaggctg ttcaaaaagc atggcataga gtttatcatc aattcatcat
     4201 tcacaaagag gaatcaggaa gatatcccga acgtatacag gcttgcaaag gaactgggag
     4261 cttccgcctg gtatatgttc atgatcgtac ctaccggaag gggagaggag atcatgaacg
     4321 agcttatctc caaggaagat tatgaggata tcctggaatg gcactaccag atggagaagg
     4381 atgaacatga tatgcttgtg aggccgacat gtgcgccgca ttactacagg gtagtgctcc
     4441 aaaaggccaa ggaagagaag gtaaaattcg aaagaaggac ccttaagttc tccacaggag
     4501 gatcgaaagg ctgtattgca gggcagttga tatgtctgat caatgtggac ggagacgtgt
     4561 tcccctgtag ttatttcccc aagtctgcgg gcaatgtcaa ggtgcagtct tttcaggaga
     4621 tatgggaaga ttccgagttg ttcaaggagc tgagggattt caagaaatac aagggcaggt
     4681 gcggatcttg tgagtatatc aatgtctgcg gcggctgcag ggccagggcg tattccctgc
     4741 atggagattt ccttgaagag gaaccttttt gtaattacat gcccgtaaag atgcagaaaa
     4801 agacaatacg aggataagac aacggaggtg gtatgaacga cacatttctt aaggcatgca
     4861 gaggagagga agtcccctat actcctgtat gggtcatgag acaggcaggg aggtacctcc
     4921 ctgaatatca ggcagttcgc gctgaggtgg attttctcac cctatgcaag acaccagaac
     4981 ttgccgcaaa ggtgacgatg cagccgatac agaggtttgg ggtggatgcc gcgatactct
     5041 tttccgatat cctcatcgcg gtggaggcga tgggaatgca tctggaattc tctgacaaga
     5101 aagggcctgt gttgggtgat cctctgagaa ccaaatctgg cgttgaccgg ctgatcgtcc
     5161 ccgatactga agacagcatg ccctttgtga tagaggcgat caagatcctg agatcacagc
     5221 tcaaggtacc cctcataggg ttttccgggg caccgttcac tcttgccacg tacatgatag
     5281 aaggggggag ctcgaagaac ttcctgaaca cgaagaaaat gatgtttcaa aaccctggac
     5341 tcttttccct cctcatggat aagatcacaa tgacggtggt atcgtacctc agcgcccaga
     5401 tcagggcagg agcccaggcc gtccagctct ttgacacttg ggcaggcatc ctggcgcctg
     5461 aggactacaa ggagtacgtc ctgccctcgg tgaaaaaagc tgttgctgag ttgaagaaag
     5521 aaggggtgcc cgtcatctat tttgtgaatg agtgcgcggc gctcttgaag gagctcaaaa
     5581 agaccggggc tgatatcata gggattgact ggagaataga cctgaaggac gctgttaaag
     5641 gacttggcaa gaaatttgtg gtccagggaa atcttgaccc gtgtgcactc ttcctccccc
     5701 gggaaaagat ggaggataag gtgaaggata tcctgtggaa aggagagttt gcaaagggac
     5761 atatcttcaa tctcggacat ggggtattgc ctgagagtcc tgtggaaaat gtcgctgcgc
     5821 tggtggaggc tgtccataga tttagtgaac ggtagtctga cgaagagacg tctcatttct
     5881 aacgccgcaa gaattgcgct cgtctatttc cttgcgagtt tcgtgtatat ttatttctct
     5941 gaccgtttgg tggaagtctt tattcaagat tttaaccttc tcacttctgt tcagaccttt
     6001 aagggtgccg cgtttgtggc attgtcatcg ctccttatct atgtgctcgt catccggaag
     6061 gagagggaaa acgagaggct cctccgtgag cttgactcca aggtgaagga gagaaccacc
     6121 aagctggaag aggcaatgct ttttgccgag tcaacgagca agctcaaatc agaatttctg
     6181 gccaacatgt cccatgaact gaggacacca ctcaatgcca ttataggttt ttctgaggca
     6241 atgctcagcg gggtgtacgg accggtcaat gagaggcaca gggaatatct cggtgatatc
     6301 ctcaccagcg gggaaaacct cctcggtctt atcaatgata tcctggatct cagcaagata
     6361 gaagctggtt ccatggatct ggaacgaagg acattttccc tgaaggaact catgaaatct
     6421 gcagtgggga tgtttaggga gaagtccgtt gcgcacaata ttacgcttga atgtcatgtt
     6481 gaggacggtg tggatgagat cgttgcagat cagaggaagg tcaagcaggt aatggtgaat
     6541 ctcttgtcaa atgccctgaa gttcaccccg gctggcggaa cagtgcgtat gaatgccaga
     6601 aaagcggctg acacaatcga ggtctccatt aaggatacgg gaatagggat atcgagccat
     6661 gacattcccc acctgttcca gccgtttcat caactggaat caccattcca gaaaaaatac
     6721 agtggtgcgg gtcttggact tttcctcacc aagaggctcg ttgagttaca ggggggggga
     6781 atccgtgtgg agagtgaaaa aggggaggga tcagtcttct ctttttcgat tcccattaag
     6841 ggcaatgtct gatatattga taaggggaag cggatgttca agggcggttg agtcataagg
     6901 gagggttgcg atcaagaaga tactcatagt ggacgacgat gaactaaacc tgaggtttat
     6961 gtcatcgatg cttgaggccc agggatatca ggtggaaatc gccagaaacg gggcggaggg
     7021 gattgagaag gtcctcagct tccatcctga cgtgacgctt cttgacatca tgatgcccga
     7081 gatggatggc tacgaggcat gcctgagact gaagaacagg gaagagacaa acaatatccc
     7141 tttgatactc gtaaccgcgc ttgatgacag ggaatcgaga cttaaggggt ttaaggcagg
     7201 tgctgatgag tttctcacaa aacccattga cagggttgag cttaccctcc gcctccagaa
     7261 tcttatcaag gtaaaggaat acggagactt tctcaaggat tacagcaaga ccctcgaaag
     7321 catggtgaat caaaggaccc tcgagctcga aaatgcatac cgtgaggtgg aaaatgccaa
     7381 tgagaggatc aagaaagggt atcttgatac catttacagg ctcaccatgg ctgcagagta
     7441 tagggacgaa gacaccggaa accacctgag gcgtataagt ttttacagcc agatgatcgc
     7501 aagagagctt gggatgtcag acgcttttgt tgataccatc tgttattcgt ccccaatgca
     7561 tgatatcggc aagataggga ttcccgatgc catcttgctt aaagcagaca ggcttacctg
     7621 cgaggagttt gagctcgcaa agacccacac catcatcggc ggcaatctcc tgaagggttc
     7681 tgaatccgag atactccaga tggcggaaag gatcgcgctg aatcaccacg agagatggga
     7741 caggaccgga tatccctcag ggctcggagg gaacggtatt cccattgagg gaaggattgt
     7801 gaccatggct gatcaatatg atgcattgag gagcaggagg ccgtacaaac cctggatgtc
     7861 ccacgaggaa gcatttgctg ttatcacaaa aggcgacggc aggacaaggc cggaaggttt
     7921 cgatcccgag gtgctggaga tcttcatcaa gcgtgaagaa gactttcgta tgatatatga
     7981 ggagcacaag gattatagat cggatcaatt aacggatagt ggatgagcag aatgcgtaca
     8041 cgctattcat tacgttaaga tgccctgtcc cgaaagcctt cgggactaca gggtttttca
     8101 ataagacttg atagcggcga atcctgtacc tggaggttgg taagagatca agaccattgg
     8161 cattctcctt ctcaatcttg gaggaccgga ctcccttcag gcagtgaagc cattcctcta
     8221 taatctcttt tctgacaggg agatcattag actggggcct gccatttttc agaaacctct
     8281 cgcacggctc atatcaacat ttcgtagaaa aaagaccgaa aaaatgtaca gccttattgg
     8341 gggaagatcc ccgatacttg agattacctt ggctcaggca gattccctgg aaaaggcgct
     8401 gaatcagaag ccggcactca ccagtcacca gtctctgaac aaagaggctg aatctatcca
     8461 taacctccag gagcctcccc aaaggagact ttttgaggtc tttgtaggga tgaggtactg
     8521 gaatcccttt atcgaagatg cagtacgaaa gatttacggt gagggaatac gggccgtgct
     8581 tgcagtcacg ctctatccac actattccct ggccacaaca ggttctgcag taaagaaatt
     8641 caacgagatc atacgccaat accctatgga agcctcatgc atttcctcat ggtacaatca
     8701 tccttactat atcgatgccc ttgtggagcg catccggcag ggagtcctct ccttccaggg
     8761 ggaagaagcg gaggtgctct ttagtgccca cagccttccc gtgagcattg tggagtcagg
     8821 agacccctat gttgatcata ttatggaaac tatcggtgag gtggtgaagg aaaccgacct
     8881 cagatggcat ctttcgtacc agagtaagag cgggcccgtg aaatggcttg aaccctcaac
     8941 cgaggatgcc ttaagggaac ttgcgaagag gggggtaaga aat
//
LOCUS       DATKOU010000094        14432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1271658, whole genome shotgun sequence.
ACCESSION   DATKOU010000094 DATKOU010000000
VERSION     DATKOU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271658"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VMU21_04320"
     CDS             <1..259
                     /locus_tag="VMU21_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76784.1"
                     /translation="QKMLIDSITKEPLVLTPDDLAALSDTLNKHLAAEAKSLELADVA
                     LNNSELFITRYILSYLIADEQKHHSLLTKLNELKRATVFVT"
     gene            complement(517..798)
                     /locus_tag="VMU21_04325"
     CDS             complement(517..798)
                     /locus_tag="VMU21_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76785.1"
                     /translation="METKTAFPGIDIKGVNDNLVHFMRSSLDATYNAANMTQDIGIQI
                     FKNVLESGKQIQTEMTKIVDVMVENTKKGTEEYKRICEEGLKTVESIYS"
     gene            complement(882..1223)
                     /locus_tag="VMU21_04330"
     CDS             complement(882..1223)
                     /locus_tag="VMU21_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76786.1"
                     /translation="MALKTISLKLDQEEYEKLKGHLSKFGDPDINVAYVLRAYIRDLN
                     RTLPLLAASGWDLKNYLGLAGMWLRQVGAITDMELFARMVPSPWSLKSGEESGDESTD
                     RKTARERGGDD"
     gene            1691..4168
                     /locus_tag="VMU21_04335"
     CDS             1691..4168
                     /locus_tag="VMU21_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO76787.1"
                     /translation="MPRMKKHEKSPVIDSFRSGWGTKGKRALCALQGMIRLVSGRLSL
                     DEMITSTLEAVGTHLSPDVALLYLREGKELSAKGVWGRNDRFRSISRNMHTVGQCLCG
                     LAAEGQAVYSDNIHADPRCIHDECRKAGLRSFAALPLMGEGTVIGVLGLGASRRRTFK
                     SQSAFLETAAGIAASAIQNTLWRAQDQRAEARLRKSEERFRDLFENATDLIQIVSPEG
                     RLLYVNRSWRETLGYSEEEIEGLSLPDIIDPTCDEHCNKTFGKILSEGVAEDIETVFI
                     AKDGRKVSLEGSGHCKYEGGKPGSVRCMFRNVTSRKAAEDALRESERKYRNLSQEFQA
                     LLNAIPDNLTLQDPDLKIVWANQGAASGLGRSVTDLEGRYCFSLWHQRLAPCASCPVQ
                     ESFRIGTPCTRQVTTPDGQVWELRSVPITDEQGVITKVVEVGRNITENSRLQAQLLQA
                     QKMESVGRLAGGVAHDFNNILSAIVGFGNLLQLKMADDDSNRVYLDNILLSAEKAAGL
                     TQSLLAFSRQQVIKPALIDINEVIRRVEKLLVRIIGEDIKFQTDLSTEKLVVMADPLQ
                     MEQVLMNLATNARDAMPGGGQLVIATDQVVFDEDYIKTHGYGRPGRYALISVTDTGMG
                     VDEEIQSRLFEPFFTTKDVGKGTGLGLAIIYGIVKQHEGYINLYSEPGAGTTFKIYLP
                     ARDTGASQEEIPKEMPLLALRGSETILLVEDDSEVRRATKTMLDEFGYSVIEAVDGVD
                     AIMKFRLHHDSIDLLLLDVIMPGKNGGEVYAEAQAINPSVRVLFTSGYPADIIKTHVV
                     LEEGLHFLSKPAAPQALLKKIRELIGE"
     gene            complement(4260..5966)
                     /locus_tag="VMU21_04340"
     CDS             complement(4260..5966)
                     /locus_tag="VMU21_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO76788.1"
                     /translation="MKKTYTTRTEVHKELEEMRKKFHEMEQCRRDFRDVLERYDQLLE
                     SAPDALIFVNRKGTIVLVNAQFERLFGYGADEVAGKPLTMLIPERFHEAHRDHVTQFF
                     AKPRVRPMGSGLTIYALKKDGTEFPADISLSPLEAEGELLVTAAIRDITERKRAEELI
                     ERNYHIQRTISAVLKISLETIPLEEQMEQVLDLIVTIPGLALESKGAIYLVEDEPDVL
                     VLKAPRKGFDEEIPCEKIAFGRCLCGRAAERCEIVFADCISDSHEVRFRGDSPHGHYC
                     VPIVSGGKTLGLINVFLREGHKRSVGEEEFLTSIAHTLAGVIERYRADQEKERLREQL
                     AQAEKLSALGRVTASVADEIRNPLTSVGGFARRLHKKLPESAPEQEYTEFIISEVDRL
                     EGILHDVLVFSRGGGPHRTETDIQEIVDDAIRPFEERCRTQSIEIRRSFGNASHVQID
                     GEQVLEAVKNLISNAIDSMPGGGTLSITTAMELRERKPYVTVKIGDTGRGIPREEITL
                     IFEPFYTTKIQPKGTGLGLPIAKKVIEDYGGFIQAESTTSGSVFSLFFPQDTTASRSG
                     VG"
     gene            6205..8157
                     /locus_tag="VMU21_04345"
     CDS             6205..8157
                     /locus_tag="VMU21_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUO76789.1"
                     /translation="MKKTVLFLINLGFLLISLFAYRLVFFFKYADPGGLKMKDIVLAF
                     LIGLRFDLSTAAYVLVPVGVCMFIPYVSLKERYLKALALFNLLLLTLVTIYLFVDLLY
                     FPFSLRHLSFELFNTKGDIMPMVKIGFAEYGFQVLCLVLFITAMGAAYYAAIRKILGK
                     FSQNGYSLTRKVIGEVVSFIVVLTLSIILARGGLQTKPLKYSDAFALNSPFLGQLALN
                     GLYTTMRTYYSMRENREMKGWDKRLCSQEEAFQESRAMIVDLQREDIPDPNYPLYRSF
                     TYRADEFRPLNVVIFIMESWSSRYVGASGGEEDATPFFSGLSKDGVFFRNYFANAQRS
                     IEAISAIITSIPPSGGMVLSQSGALSQMPGKFLPLLLREKGYNTFFVHGAKRGSMGFD
                     ALVRQTGIEQYISKEDILKEGGRDDGVWGIYDEDTFLYSHRLFEAQTKPFFAVIFSLS
                     SHTPYELPAEKFHFYKPERPFYDFLNSLRYSDYALSRFFDAARNTHYFSNTVFIIVGD
                     HTEGKSVGNSLSQRFSVPCLIYSPRHIKPVRVTKTVTHVDLAPTLLDILRSSDAHASF
                     GQSAFINSPGTGLLPYGDFDIFVREGWMLVSSPQGVMETHNYLGESSGQEKLSRLKKE
                     EGCYLQFFHDLILSNRLYPMAGKATR"
     gene            complement(8217..8711)
                     /locus_tag="VMU21_04350"
     CDS             complement(8217..8711)
                     /locus_tag="VMU21_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUO76790.1"
                     /translation="MSGYKGSTEIAKTERPKYLAPFEEMERWFEDVFRSPLSLLSRRT
                     WPETSALAEFETLSPSVDIFEEGNEVVLKADMPGIEKKDIDITLSDNMLTISGEKTHE
                     DKVEEGSYVRCERTHGSFFRRFELPSDIDAEKIKAHLDNGVLEVRLPKTEESKSRSKK
                     ITVS"
     gene            9101..9685
                     /locus_tag="VMU21_04355"
     CDS             9101..9685
                     /locus_tag="VMU21_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HUO76791.1"
                     /translation="MKAVAFNGSARKDGNTAILLNTVLGELKRGGIETELVQLSGKSL
                     QGCIACYKCFENKDGRCAVQKDEMNVYIEKMRRADGILLGSPTYFSDVSTNMKALIER
                     CGMVARANDDMFRRKVGAGVVAVRRAGAAHVFSSLNFFFLIGQMIIPGSSYWNIAIGR
                     QAGEVMNDNEGMQTMTNLGTNMAWLMKKLSNQDL"
     gene            9698..10807
                     /locus_tag="VMU21_04360"
     CDS             9698..10807
                     /locus_tag="VMU21_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HUO76792.1"
                     /translation="MGRSRVIIRRASYDYLLLKPLIFEMADSLCGEVVQSRSRVVIKP
                     NLLAPARPERAMLTHPLVVKGVAEYCLQRGAYVQISDSQAIGSFDRVIKESGLGRALE
                     GMDVVCKEFRESVKVDVGEPFNKIEIAKDVLEADVLINLPKLKTHVQMLMTLGVKNLF
                     GCIVGLRKPEWHFRTGVKREAFAQLLVRIHNAVRPSLSILDGILAMEGEGPGRSGSPR
                     KLGVLAGSNDAVALDMTICRMLGREPHSLFTNKAAEDMGYVPDIEVLGEAPAVRNFKF
                     PEMAPLVFGPRSLHGFMRRHLVQRPVPDGSLCRLCGECGNYCPAKAISFDEKRIHFDY
                     DKCIRCYCCIEVCPHGALQAEEPLMGKAIRKLRKS"
     gene            11014..12609
                     /locus_tag="VMU21_04365"
     CDS             11014..12609
                     /locus_tag="VMU21_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUO76793.1"
                     /translation="MSVLSCSFSAYAYTDYGDTVQVTVVTNDNLITICKKLLEEPRKW
                     GIIAKINKLQDPDLIFPGQKLTIPADMLRGVPLDGMVTFLSGEVRAQAKEGEEWKTLH
                     INDRVKQGSSIKTGDGSGVEISFEDGDSFLMRSNSALMIRIAKKRGMMNFLQRLFLSA
                     GRTITKVREATGRKSRFEIQTPSAVAVARGTSFRVGVDEKETMRSEVLKGFVDVEAMA
                     KRVEVQGGEGTLARKNEPPVQPRKLLPPPMLLHREPLYKAMPLVFQFSEIEGALFYRV
                     MVARDDAGKDVVKEKVIKPGEKAEIAGVDDGEYFLISQSIDAEGLEGLPSDPVAFRLR
                     INPLPPVFQSPGDGASLRAKKMDFRWLSVNDAVSYHMQIAEDKGFTRIVVDRSGIAAP
                     ELTSPSLDYKTYFFRVSSIARDGYEGIFSPARSFTLVSPPPAPVIEKPEMDTKEIRLR
                     WRSLGEGVTYRFQMSMDSEFKNMLIDREMEMPEMTLPRPKEGGKYYFRAKGIDREGYE
                     GDFSLPQSYQIQKIEGLLYWLVF"
     gene            12652..>14432
                     /locus_tag="VMU21_04370"
     CDS             12652..>14432
                     /locus_tag="VMU21_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HUO76794.1"
                     /translation="MGPLSKKTIRYILITVVGVILILCAEQLGFFEGIDTYIYDTSFR
                     LRDPRTPSDKIIIVAIDERSLDALGRWPLRRIYYARLLDRLREAKVVSFDLILSEQSD
                     DDALLAEAMQRHGRVILSVYVGTEMERVYPLGLLSPYKVGHVHLEQGVDNVAREIFHT
                     LHFKDLQLPSLSSVTYETLSGTLLHRSQVPHKTQYQPSQDSVFQADFAKINYYGIPGT
                     FQQVSLSDAIEGRYPSAFFRGKAVFVGVAAQGIGDKVNTPLSQQRNRMSGVEVHANIL
                     NNLLDNSSITDVNLWIRWGVCVLFSLLCFLIFMKFSEKISALGWFLSLFIIATSSFYL
                     FSSLNLWLAPAMFYFSFTYVYLMTYLFKLDTAARRLDEEFSSVTVLLAKTAEGNGKGA
                     SDKGLISLLSPGGINAKIQGLIRVEQDYGRTLEDTVQKRTEELASALSMLGSVSNEMI
                     LRLTRAVESRDEDTGGHVARIGLYCKVIAEELGMPADFVESIPFSSALHDIGKIGIPD
                     SILLKASTLTPQEETLMQSHTIVGAKILANSAYPKIQMSASIALCHHERWDGTGYPHG
                     VKGNEIPIDARIVTICDHYDAIRSRRR"
BASE COUNT         3428 a         3491 c         3874 g         3639 t
ORIGIN      
        1 gcaaaagatg ctgatcgaca gcattaccaa ggaacccctt gtcctcacac cggatgatct
       61 tgcagcccta tcggataccc taaataagca tcttgctgca gaggcaaagt cccttgaact
      121 tgcggatgtg gcacttaata acagcgagct tttcatcacc cggtacatcc tctcgtacct
      181 tatcgcggac gagcagaagc accatagcct cctcaccaag ttgaacgagc tgaagagggc
      241 gacagtattt gtcacttaaa cttttccgtg aatagtgggc ctccttcccc tcaaaagcga
      301 aaagaaggtc ctgaagtgta ttggaaggtt tgaaggcgac aagatgaaaa gtgtaactca
      361 ctgcggcaag agaaggacca tactgcatgg atttccttta cttccacctg ccggcggata
      421 acacggactc tttctgggtt gcgatgatga aaaaggtggt ttttgggaaa gaatagggat
      481 gcgctttagg cgcatcccct gggggaacta cctggattag ctgtagatgc tttccacagt
      541 cttgaggcct tcttcgcaga tcctcttgta ctcttccgta cccttttttg tattctcaac
      601 catgacatcc actatctttg tcatttcggt ctggatctgt ttgcctgatt caagaacgtt
      661 cttgaatatc tggattccga tatcctgcgt catgtttgcg gcgttgtagg tggcatccag
      721 agaagacctc atgaaatgca cgagattgtc gttaacgcct ttaatgtcga ttccggggaa
      781 tgctgtcttg gtttccattt tcgtttctcc ttattttctt gagatagtgt ttagcgcgac
      841 aatgtcgcgg cggtgaacag tgtgcccctt ttttcctttc cttagtcatc accccctctt
      901 tccctggcgg tcttccggtc agtactttcg tcaccagact cctccccgct tttcagagac
      961 catggggagg gcaccatcct ggcgaacagt tccatatcgg taatggcgcc gacctgcctc
     1021 agccacatac ctgccagtcc gaggtagttt ttcaggtccc atccggaggc ggccaggagc
     1081 ggtaaggtcc tgttaaggtc gcgtatgtaa gccctcaaca catatgctac gttgatgtcg
     1141 ggatccccga atttgctgag gtgtcccttc agtttctcgt attcttcctg atccagtttc
     1201 agactaatgg tcttaagtgc catctttagt atctctttag tattattata gtaatactaa
     1261 cgtaacttgt caagagtttt ttttgaaaga tgtagaaacc taaattgtga cataacatat
     1321 tgatattaca tatttttaat gataggaaag gcatcattta tgatccttgg acccccgtag
     1381 cacacataac aggtatggct ggacatctcc ctaccataaa aatggttatg gagtaggaaa
     1441 aacagggata catgtatagt tttcaaacgg ggtgtatgaa aatctggcat tggaaacttc
     1501 tcgatggtgt ggccgggaat taggtgctca catattttta ttaaatttca aagtgataca
     1561 ccaccttccc caccaggcat cctaattgct aacaagataa tgatagaaaa ctgttcttgc
     1621 cgcatacaga ggagggagaa catggcattg ctaagtggcc acttgttcgg gggccctcga
     1681 ttatgaccat atgcccagga tgaagaaaca cgagaagtcg cccgttatag acagtttccg
     1741 ctccggttgg ggaacgaagg ggaagcgggc actctgtgcg cttcagggga tgatccgctt
     1801 ggtgagtggc aggctgtccc tcgacgaaat gataacgtcg accctggaag cggtaggaac
     1861 gcatctgtct cctgatgtgg cacttctcta tctgagggaa ggaaaggagc tttcggcaaa
     1921 aggagtctgg ggcagaaacg accggttccg aagcatcagc aggaatatgc ataccgtggg
     1981 acaatgtctc tgcggactcg cggcagaggg ccaggccgtt tactctgaca atatccatgc
     2041 cgatccccgc tgcatacatg atgaatgcag gaaagcagga ctgcggtcct ttgctgccct
     2101 gccactaatg ggtgagggca ctgtcatagg ggtgctgggg cttggggcca gccgcagacg
     2161 tacgttcaag agtcagtcag cctttctcga aacagccgca ggcattgctg cgtcagcaat
     2221 ccaaaatacg ctctggaggg cacaggacca gcgcgcggaa gcgagattaa gaaaaagcga
     2281 agagcgcttc cgtgacctct tcgaaaacgc tacggatctt atccaaatcg tttctcccga
     2341 gggccgcctc ctctacgtga accgttcatg gcgtgagacc cttggatatt ccgaagagga
     2401 gatagaggga ctaagcctcc ctgatatcat cgaccccacg tgcgatgagc attgtaataa
     2461 gacgtttggc aaaatactgt ctgagggtgt cgccgaggat atcgagacgg tctttatcgc
     2521 caaggacggc aggaaagtct ctctggaagg gtctggtcac tgcaaatacg agggagggaa
     2581 accggggtcg gttcggtgca tgtttcggaa tgtgacttcc cggaaggctg cagaggatgc
     2641 gctgcgggag agcgagcgga agtaccggaa cctctcgcag gagttccagg ctctccttaa
     2701 cgcgattcct gacaatctta ccctgcagga tcccgacttg aagatcgtct gggcgaacca
     2761 gggagcagca tccgggctcg ggaggagcgt caccgacctt gaaggcaggt actgcttctc
     2821 cctctggcat cagcgcttgg ctccctgtgc gtcctgtcct gtgcaggaga gctttcgcat
     2881 cggtacaccc tgcaccaggc aggtcaccac acccgacggt caggtctggg aactgaggtc
     2941 ggttcccatc acggacgagc agggcgtgat cacaaaagtg gtcgaggttg gccggaacat
     3001 cactgaaaat agccggctgc aagcccagct tctccaggcc cagaagatgg agagcgtagg
     3061 ccggcttgca gggggcgtgg cacatgactt caacaacatc ctctccgcca tcgtgggatt
     3121 cggcaatctt cttcagctca agatggcgga cgacgattct aatcgggtgt acctcgataa
     3181 tatcctcttg tccgcggaga aggccgctgg tctgacacag agccttcttg ccttcagcag
     3241 acagcaggtg atcaagccgg cgcttatcga catcaatgag gtcatcaggc gggtggaaaa
     3301 gcttttagtg aggatcatcg gggaggacat taagttccaa accgaccttt caaccgagaa
     3361 gcttgtagtg atggctgacc cgctccagat ggagcaggta ctcatgaacc tggctacaaa
     3421 cgcgcgggac gcaatgcccg gcgggggtca gttggtgatt gccacagacc aggtggtgtt
     3481 cgatgaagat tacattaaaa cccatgggta cggcaggccc ggcagatatg ctctgatttc
     3541 agtcaccgat acggggatgg gcgtggatga agagatccaa tcgcggctct ttgagccttt
     3601 cttcaccaca aaggatgtgg gaaaaggcac aggcctcggg ctcgccatta tctatggtat
     3661 tgtgaaacag cacgagggct acattaatct gtacagcgag ccgggtgccg ggactacatt
     3721 taagatctac ctgccggcga gagacaccgg ggcatcacag gaggaaattc ccaaggagat
     3781 gccactgctg gcactgcgtg gatcggagac catcctcctt gtggaagacg acagtgaagt
     3841 cagaagggct accaagacaa tgctggatga attcggctat tcggtgatcg aggccgtgga
     3901 cggtgtggat gccataatga aattcaggct gcaccacgat tccatagacc ttctcctcct
     3961 ggatgttatc atgccaggga aaaacggcgg ggaagtctac gctgaagcac aggcgatcaa
     4021 tccctctgtc agagtgcttt tcacgagcgg atatccggct gatatcatca agacccatgt
     4081 tgtcctcgaa gaaggactgc atttcctctc caagcctgca gcaccccaag cgctcctaaa
     4141 aaaaatcagg gagttaatag gagaatgacg gaaatcagtg cacacgccct gtcctgatgc
     4201 ggtgaggctc ataccgtagg gcgagaatat ccaccccttg ctttatgtcc aaccagaggt
     4261 catccaacgc cggatcggct tgcggtggta tcctgcggga aaaagaggct gaagaccgag
     4321 cccgaggttg tgctttctgc ctggatgaac ccgccgtaat cttcgatgac cttttttgcg
     4381 atgggaaggc cgagacccgt gccttttggc tgtatcttgg tggtatagaa cggctcgaaa
     4441 ataagtgtta tctcctcacg gggaatcccc cttccggtat ctccgatctt caccgtgaca
     4501 tagggtttcc tctctctcag ctccatggcg gtagtgatac tcagcgtgcc cccacccggc
     4561 atggagtcaa tggcgttgga gatgaggttc ttcaccgcct ccagaacctg ttccccgtcg
     4621 atctggacat gggacgcatt cccaaaggat cttcgtatct ctatcgactg ggtgcggcac
     4681 cgctcctcga atggtcttat cgcatcatcg acgatctcct gaatgtctgt ctctgtcctg
     4741 tgcggtcctc ctccccggga aaagaccagg acatcatgga ggatgccttc gagccggtct
     4801 acctcagaga tgatgaactc cgtatattcc tgttccgggg cgctttcagg gagcttcttg
     4861 tgaagcctcc tggcgaaccc gcccactgag gtgagagggt tcctgatttc gtccgccacg
     4921 ctggcagtga cccttcccag cgcggagagc ttttccgcct gtgcgagctg ctccctgagc
     4981 cgctccttct cctgatccgc gcgatagcgt tcaataacgc cggcaagggt gtgtgcgatg
     5041 gaggtgagga actcttcctc tcccacgctt cgcttatggc cttccctgag gaagacattg
     5101 ataagtccca gggttttgcc tcctgagacg atgggcacgc agtaatggcc atggggcgaa
     5161 tcgcctctga aacgcacctc gtggctatcg ctgatacagt cggcaaagac gatctcgcac
     5221 cgttctgcag cgcggccgca aaggcatctg ccaaaggcga tcttttcgca gggtatctct
     5281 tcatcaaacc ccttgcgcgg ggccttgagt accaggacgt cgggctcgtc ctccacaaga
     5341 taaattgcgc cctttgactc gagggcaagg ccggggatgg tcacaatgag atcgaggacc
     5401 tgctccatct gctcttccag ggggatggtc tccaaggata tcttgagaac cgcgctgatg
     5461 gtcctctgga tgtggtagtt cctttcgatg agttcctctg cccgctttcg ctcagtaatg
     5521 tcccgtatgg ctgcggtcac gaggagctct ccttcggcct caagggggct cagactgata
     5581 tctgcgggga attcagtacc gtcttttttc aaggcgtaaa tggtgaggcc agagcccatg
     5641 ggtctcaccc ggggctttgc aaagaactgt gtcacatggt cgcggtgtgc ctcatggaaa
     5701 cgctcaggga tgagcattgt gagtggtttc cccgccacct cgtctgcgcc atatccgaag
     5761 agccgctcaa actgggcatt taccagcacg atggtccctt tgcggttcac aaagatgagg
     5821 gcgtcggggg ctgactccag gagctggtca taccgctcca gcacatcccg gaaatctctg
     5881 cggcactgct ccatctcatg gaacttcttc cgcatctctt caagttcctt gtgcacttca
     5941 gtcctggtgg tataagtctt tttcatctca ctatgctcct cataaccgag ctttcataag
     6001 gatatccgtt aagcgaattg aaatagaaaa gggccggatc gaaaaaccgg ccttttttgt
     6061 tggaagtatt gcgatgccct cctcggcgtt cagattgaga aaaatatatc aatgggaagc
     6121 atgaatgtca aggaaggagt tcatgactga gtcctcccct ttcatccctc cgttacataa
     6181 tggtattata aaaaaccgaa tatcatgaaa aaaactgttt tgtttcttat taatctcgga
     6241 ttcttgctga tctccctctt tgcctacaga ctcgtcttct tctttaagta tgccgatccc
     6301 ggcgggttaa agatgaagga tattgtcctc gcttttctta tcggactgag gtttgacctc
     6361 tcgactgctg cctatgtatt ggttcccgta ggtgtatgca tgtttatccc gtatgtttcg
     6421 ctgaaagaga ggtatctgaa ggccctggcc ctttttaatc tgctcttgct cacactcgtc
     6481 accatttacc tttttgtgga tctgctgtat ttcccctttt ctctgagaca tctcagcttc
     6541 gagctcttca ataccaaagg ggatatcatg ccgatggtta agatagggtt cgcggaatac
     6601 ggatttcagg tcctctgtct tgttctcttc attacggcga tgggtgctgc atactatgca
     6661 gctatcagaa agatattggg gaagttctcc cagaatggat attcattgac caggaaggtc
     6721 attggtgagg tggtcagttt catcgtggtg ctcacccttt ccattatact ggcacggggt
     6781 ggtctccaga cgaagcctct caaatacagt gatgcctttg ccctcaattc tccttttctg
     6841 gggcagctcg ccctgaacgg gctttacacc accatgagaa cctactacag catgagggaa
     6901 aacagggaga tgaagggatg ggacaagcgt ttgtgttcgc aggaagaagc gttccaggag
     6961 agccgagcga tgattgtgga tctgcaacgg gaggatatcc ccgatccgaa ctatcccctc
     7021 taccgctcct ttacctatag ggccgacgaa ttcaggcccc tgaatgtggt gatcttcatt
     7081 atggaaagct ggtccagcag atacgtgggt gcatcagggg gcgaggagga tgccacgccc
     7141 tttttcagcg ggctttcgaa ggacggggtt tttttcagga attattttgc caatgcacaa
     7201 cggtccatag aggccatttc cgcgatcatt acttctatcc cgccgtcagg cggaatggta
     7261 ttgtcgcaaa gtggcgccct ttcgcagatg ccgggaaaat ttctacccct tctcttgaga
     7321 gaaaaggggt acaacacctt cttcgtacat ggtgcaaaac gaggttccat gggcttcgac
     7381 gctctggtaa ggcagacagg aattgaacag tatatctcga aagaggatat cctcaaagaa
     7441 ggggggaggg atgacggtgt ctggggtatc tatgacgaag atacctttct ttacagccac
     7501 aggctttttg aggcacagac caagccattt ttcgcggtga ttttttccct tagcagccat
     7561 accccttatg agcttcctgc agagaagttt catttctata aaccggaaag gcctttttat
     7621 gatttcctga attccctcag atattcagac tatgccctct cccggttctt cgatgcagcc
     7681 aggaacacac actatttctc caacactgta tttatcattg tgggggacca tactgaggga
     7741 aagtctgtgg gaaacagcct gtcccagaga ttctcggtgc cctgtttaat ctattcgccg
     7801 cgtcatatca aaccagtgcg tgtcacgaag acggtgaccc atgtagatct tgcacctact
     7861 ttgctggata tcctgaggtc ctcggatgcc cacgcctcgt ttggtcaatc tgcctttatc
     7921 aattcccctg gaacaggtct gctgccctat ggcgactttg atatttttgt gagggagggc
     7981 tggatgctcg tatcttcccc tcaaggggtc atggaaacgc ataactatct cggggaatct
     8041 tcggggcagg agaaactctc aagactaaaa aaagaagaag gctgttacct ccaattcttt
     8101 cacgacctaa tcctttccaa caggttatat cccatggcgg gaaaagctac gcggtgaaca
     8161 gacttgggag agcagacaaa gaaactcccc ggcattggcc gaggagttgt atgaaatcac
     8221 gaaaccgtga tttttttgct tctgctcttt gactcttcgg tcttggggag tctcacttcc
     8281 aacactccat tgtcaagatg agcctttatc ttttccgcat caatatccga aggaagctcg
     8341 aacctcctga agaaggaacc atgggtacgc tcgcacctga catagctccc ttcctcaacc
     8401 ttgtcttcgt gcgttttttc tccggaaatg gtgagcatgt tgtcggagag cgtgatgtcg
     8461 atatcctttt tctcgatacc cggcatatca gccttcagga ccacttcatt cccttcctca
     8521 aagatatcca ctgaaggtga aagggtttcg aactctgcca gcgcacttgt ttcaggccat
     8581 gtgcgtctag ataggagcga aagggggctc ctgaacacgt cctcaaacca tctctccatt
     8641 tcctcaaaag gcgcaagata ttttggtctt tcggtctttg ctatttctgt tgatccttta
     8701 tatcctgaca tcgtagtcac ctccgcgaag ggcaatttcc tttcggagtt tcaaccctcc
     8761 tgatttcata gtataactaa gtagttatat ttgcaactat tgaaatgcac agatttcggg
     8821 cgttttttgt cctttccgga actgaaagat gaatcttcga cctttttctt tcttgattga
     8881 atgtatcttt ggcgtggttc attctatctc cttttttttc tttcaaacct actttgcgtt
     8941 cgagggttcc ggtgaatgtc ccacctgcag ccttttttta agtgacgggt gttttctcct
     9001 cacgtggtca cccaatccac agggcccttt tattgctttt ctctcagttg attgtttaaa
     9061 ttcatagaaa aacaaatcct tacatttgtg gaggtgcagg atgaaggctg tggcatttaa
     9121 tggaagcgct cggaaggacg gaaataccgc cattcttttg aacacggtac tgggggagtt
     9181 gaagagagga ggcatagaga ccgaactggt ccagctttcc gggaagtccc tccaggggtg
     9241 tatcgcctgt tataaatgtt tcgagaacaa ggacggaagg tgcgcggtac agaaggatga
     9301 aatgaatgtc tacatcgaaa agatgcgaag agcagacggc atactcctcg gttcgcccac
     9361 atatttctct gatgtgagca caaatatgaa agccctcatt gagaggtgcg gcatggtcgc
     9421 ccgtgcaaac gacgatatgt tcaggaggaa ggtgggcgca ggggtagtgg cggtccggcg
     9481 tgcaggggct gcccacgtat ttagttccct taactttttc tttctcatcg gccagatgat
     9541 tattcctggt tcgagctact ggaacattgc aatcgggagg caggcgggag aggtgatgaa
     9601 tgataatgag gggatgcaga ccatgacaaa tctggggacg aatatggcct ggcttatgaa
     9661 gaagctcagt aaccaggacc tctgaaaagg cgaagcagtg ggcaggtcca gggtcataat
     9721 aaggcgggcg tcttacgact atctcttact caaacccctt attttcgaga tggcggattc
     9781 attatgcggg gaggtggttc aaagccgcag cagggtggtg atcaagccca atctcctcgc
     9841 gcctgcccgg cccgaaaggg caatgctgac gcatcccctg gtggtgaagg gagttgcgga
     9901 gtattgtctc cagaggggag cgtatgtcca gatatccgac agtcaggcca ttgggagctt
     9961 cgacagggtt ataaaggaga gcggactggg gagggcactt gagggaatgg acgtagtgtg
    10021 caaagagttc agggaatctg tgaaggtgga tgtgggagag ccctttaata aaatcgagat
    10081 tgcgaaggat gtcctggagg cggatgtcct gattaatctg ccgaagctca agacccatgt
    10141 gcagatgctc atgacactgg gggtaaaaaa tctctttgga tgcattgtgg gattaagaaa
    10201 accggaatgg cacttcagga caggggtcaa gagggaagcc tttgcacagc tcctggtcag
    10261 gatccataat gcggtcaggc cttcgctcag cattcttgat ggtatcctcg ccatggaagg
    10321 ggaagggccg ggaaggagtg gaagcccccg taagcttggg gtgctggcag ggagcaatga
    10381 tgcggttgca ctggacatga ctatatgcag aatgctcggg cgggaaccgc acagcctgtt
    10441 caccaataag gctgcggaag atatgggata tgttcctgac atagaggtcc ttggcgaagc
    10501 acctgcggtt cgcaatttta aattcccgga aatggctccc cttgttttcg gtcccaggag
    10561 cctgcacgga tttatgagaa gacatctagt ccagaggccg gtccctgatg gttccctgtg
    10621 caggctgtgc ggagagtgcg ggaactattg cccagcaaag gcgatttcct ttgatgaaaa
    10681 aaggatccat tttgattacg acaaatgtat ccggtgttat tgctgcatag aagtctgtcc
    10741 ccatggggca ttgcaggctg aagaaccgct catgggcaag gcaataagaa aactcaggaa
    10801 atcatgataa cgatttacac ttttattatt tttaaaaaac aaaatttcct gctatgatgg
    10861 tgctattaca ggtgagcgtg gtttgcattt tcatattgag gaattgtccc catgggttca
    10921 ttttcttgag ggaaatttgg tagggtctca atgatcgggg taactacacg ggagggcacc
    10981 atgaaggttc ttgtcttctg cttcgcgttg tgtatgagtg tactgtcttg ctctttctct
    11041 gcatatgcat atacagatta tggtgacact gttcaagtta ccgtggtaac aaatgacaac
    11101 ttgatcacta tctgcaaaaa acttcttgaa gagccccgca aatggggcat aatcgcgaaa
    11161 ataaacaagc ttcaggatcc agatttgatt tttccaggac aaaagctgac cattcccgca
    11221 gacatgctgc gtggcgtccc cctggatggc atggtcacct ttctaagtgg cgaggtgagg
    11281 gcacaggcaa aggaaggcga ggagtggaag acgcttcata tcaatgaccg ggtaaagcag
    11341 gggagcagta tcaaaactgg tgatggaagc ggagtcgaga tcagctttga ggatggtgac
    11401 tctttcctga tgaggtcgaa tagcgccctc atgatcagga tcgcaaagaa acggggcatg
    11461 atgaattttc tgcagagact gttcctcagt gcagggagga ccattaccaa ggtgagagag
    11521 gctacgggca gaaaatcccg ttttgagata cagaccccat cggcagtcgc cgttgcccgg
    11581 ggtacgtctt tcagggtcgg agtggatgag aaagagacca tgagatccga agtattgaaa
    11641 ggttttgtgg atgtggaggc aatggcaaag agagttgagg tgcagggggg agaaggaaca
    11701 cttgccagaa agaacgaacc cccagtgcag ccgcgcaaac tcctccctcc gccaatgctt
    11761 ctccacaggg aacctcttta taaggcaatg cctcttgtct ttcagttttc tgagatcgag
    11821 ggggcgttgt tctatagggt catggtcgca agggacgatg cgggaaaaga tgtcgtgaaa
    11881 gaaaaggtga taaagcccgg tgagaaggcg gaaattgccg gggtggacga cggcgagtat
    11941 ttccttataa gccagagcat tgacgctgag ggactggaag gattgccctc agaccctgta
    12001 gcgttccggc tgaggattaa tcccctgcca cccgttttcc agtcaccggg tgatggcgcc
    12061 agtcttaggg cgaagaaaat ggatttcaga tggctcagcg taaacgatgc agtgagctat
    12121 catatgcaga ttgctgagga caaggggttt acccgtatcg tggttgacag gagcggcatt
    12181 gctgcgccag agcttacctc gccctccctc gattacaaga cctatttctt cagggtgagt
    12241 tccatcgcca gggacggcta tgagggaata ttctcacctg cccggagttt taccttagtc
    12301 tcgcctccgc ctgcgccggt gatcgaaaag ccggagatgg acactaaaga gattcgtctc
    12361 aggtggagga gcctgggaga gggagtcacc tatcggttcc agatgtccat ggacagcgaa
    12421 tttaagaaca tgctgataga cagggaaatg gaaatgccgg agatgacact cccaagaccc
    12481 aaggagggag ggaaatacta ttttcgtgca aaaggcattg atcgggaagg atatgaaggg
    12541 gatttctcgc tgccccagag ttaccagatt cagaaaattg aggggttact ctattggctt
    12601 gtcttctgac cgtatggctt tatctctctg catggtaacg gtgaagattt agtgggccct
    12661 ttatccaaga aaacgatacg ctacatcttg atcaccgttg tcggggtgat cctcatcttg
    12721 tgtgcagagc aactgggctt cttcgaagga attgacacgt acatctacga cacctcattc
    12781 aggctgcgcg atcccaggac cccttccgac aagattatca ttgtggcgat agatgaaagg
    12841 agtcttgacg cattaggcag gtggcctctg aggaggatct attatgcccg cctcctcgac
    12901 aggttgaggg aggcgaaggt ggtgagcttt gatcttatcc tgtccgagca gtctgatgac
    12961 gacgcactcc ttgcagaagc gatgcaaaga cacggaaggg tgatattatc cgtgtatgtt
    13021 gggacggaaa tggagagggt ctacccatta gggttattat cgccatataa agtcggacat
    13081 gtgcatctgg agcagggagt ggataatgtt gctcgggaga tattccatac cctccatttc
    13141 aaggaccttc agcttccctc tctctcttca gttacgtatg agacgttgtc cggtacacta
    13201 ttgcaccgaa gtcaggtgcc acacaagact cagtatcagc cctcgcagga cagcgtattt
    13261 caggctgact tcgcaaagat aaactattac gggattcccg gtacgtttca gcaggtttca
    13321 ctctccgatg caattgaggg cagataccct tcagcctttt ttcgcggcaa ggccgttttt
    13381 gtcggagtcg ctgcgcaggg catcggagac aaggtgaaca cccccctcag tcaacaaagg
    13441 aacaggatgt ccggcgttga ggtccatgca aacatactga acaacctcct tgataacagc
    13501 tcaataacag atgtgaacct ctggattcga tggggtgtct gtgttctttt ctccctcctc
    13561 tgttttctga tcttcatgaa gtttagtgaa aagatttctg ccttgggttg gtttctgagt
    13621 ctttttataa tagccacatc gtcattttat ctcttctctt ccctgaacct ctggctcgcc
    13681 cctgcaatgt tctacttttc gttcacgtat gtgtatctca tgacatacct ctttaaactc
    13741 gataccgcgg cacggaggct ggatgaagaa ttttcatccg ttacggtcct tcttgcaaag
    13801 actgcagaag ggaatgggaa gggggcatcg gataaggggc ttatcagtct tctctctccc
    13861 ggtggaatca atgccaagat ccaggggctt atccgggtgg aacaggacta tggaagaaca
    13921 cttgaggata cggtgcagaa acggaccgag gaactggcca gtgccctgtc tatgctcggc
    13981 agtgtgagca atgagatgat cctgcggctc actagggcgg ttgagtcaag ggatgaagat
    14041 acaggtggcc acgtcgcgcg gattgggctt tattgcaagg tgattgcaga ggaactcgga
    14101 atgccggcgg attttgttga gtcgatacct ttttcaagtg cgctgcatga tattggaaag
    14161 atcggcatac ccgatagtat acttctgaaa gccagtacac tcaccccaca agaagagacc
    14221 cttatgcagt cacacacgat tgtcggggcg aagatccttg cgaactcggc ctatcccaag
    14281 attcagatgt ctgcgtccat tgccctctgt catcatgagc gatgggacgg tacggggtat
    14341 ccccatggag tgaagggaaa cgagataccc attgatgcaa ggatcgtcac gatctgtgac
    14401 cactacgatg caatacggag cagaaggcgg ta
//
LOCUS       DATKOU010000095         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1274220, whole genome shotgun sequence.
ACCESSION   DATKOU010000095 DATKOU010000000
VERSION     DATKOU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274220"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2285
                     /locus_tag="VMU21_04375"
     CDS             <1..2285
                     /locus_tag="VMU21_04375"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HUO76795.1"
                     /translation="LEALRNLSSPRALVLREREQKRIPGREVVRDDMIVLSEGDRVPA
                     DAVLISCVSLLVDESLLTGESVPVRKTFCDDESKMARPGGDDLPFVYSGTMVVQGHGV
                     AKVLATGIRTEIGRIGKALRTVETEETILQREIARIVRNLAVLGLSLCAVVVIVYGLT
                     RDNWLNGLLAGITLAMATLPEEFPVVLTVFLALGAWRISRKGVLTRRVPAVETLGSAS
                     VLCVDKTGTLTLNRMSVQKIFSNRKCYDLNHPNEPLPEDCHEVVEFGILASHIDPFDP
                     MEKALRELGRRSLSDTEHLHDNWTLVQEYPLSQNLLTMSRVWRSPDERDYVIAAKGAP
                     EAIADICHMEEREMQKLSEQINTMAEEGLRVIGVAKSEFRQTSLPDGQHEFRFRFLGL
                     IGLADPVRPGVAEAIRECYGAGIRVVMITGDYPVTARKVAVQIGLKQMDGIVTGQEIE
                     SMDDSELRERIRTVNVFARVVPEQKLKIVDALKANGEVVAMTGDGVNDAPALKSAHIG
                     IAMGGRGTDVAREASALVLLDDNFSSIVQAVRMGRRIFDNLKKAMAYIFAIHIPIAGM
                     SLLPVLFRWPLVLLPVHIVFLELIIDPACSVVFEAEPEEAHIMKRQPRNLLEPLFDAR
                     TVTYSVLQGASVLCIVLAVFLTAMYRGQGEADARTLSFTTLIIANLGLILTNRSWSRV
                     ILSTLRTPNAAFWWVLGGALFFLGMVLYVPFMQRLFHFQMLHPLDLMICLLAGIISVV
                     WFEVLKFVRRYRMYPLSTNN"
     gene            2408..2614
                     /gene="rpsU"
                     /locus_tag="VMU21_04380"
     CDS             2408..2614
                     /gene="rpsU"
                     /locus_tag="VMU21_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HUO76796.1"
                     /translation="MEIRVSGNDIEKALKLLKRQLQKDGLLKELKKRRFYEKPSVKMK
                     SKQREAQKRRVKTSRMARQNRPVS"
     gene            2717..>2901
                     /locus_tag="VMU21_04385"
     CDS             2717..>2901
                     /locus_tag="VMU21_04385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUO76797.1"
                     /translation="MNYNRFYEFGLSDEVLKAVFDMGFEEPTPIQKIAIGHALRGHDV
                     IGLAQTGTGKTAAFGIP"
BASE COUNT          669 a          703 c          845 g          684 t
ORIGIN      
        1 cccttgaggc cttgcggaat ttatccagcc ccagggcttt agtgttgagg gagagggagc
       61 agaagaggat acctggccgt gaggtggtca gagatgatat gatcgtgctt tcagagggtg
      121 atcgtgttcc cgcggatgca gtgctcatct catgcgtgag tcttctggtg gatgagtcgc
      181 tcctcaccgg tgagtcggtc cctgtgagaa agaccttctg cgacgacgag agcaaaatgg
      241 cccggcccgg gggtgacgac cttccctttg tgtattcagg gacaatggtg gtgcagggtc
      301 atggtgttgc gaaggtcctc gcaacaggga tcaggaccga gataggcagg ataggaaaag
      361 cgctccggac cgtcgagacc gaagagacca tccttcagag ggagattgca agaattgtgc
      421 gcaacctcgc tgtgctgggt ctctctcttt gcgcagtggt ggtcattgtc tacgggctca
      481 caagggacaa ctggctgaac ggtcttctgg caggcatcac cctcgccatg gccacattgc
      541 ctgaggaatt ccctgtggtg cttaccgtgt tccttgccct gggcgcgtgg aggatttccc
      601 ggaaaggggt gctcacccgc agggtgcctg ctgtggagac ccttggttct gcctctgtgt
      661 tatgtgtgga caagaccggc accctcaccc tgaacaggat gtctgttcag aaaatcttct
      721 ccaacaggaa atgttacgac ctgaatcatc cgaatgagcc tttgcctgaa gattgccatg
      781 aggtggtgga gttcggcatt ctggcaagcc acatcgaccc ctttgatccc atggaaaagg
      841 cgttgcggga gttgggcaga cgctctctgt ccgataccga gcacctccat gacaactgga
      901 cactggtgca ggaatatccc ctttctcaaa atttgctcac catgtcacgg gtctggagat
      961 cacctgacga gagggactat gtgattgctg ccaagggcgc gcccgaggct attgcggaca
     1021 tatgtcatat ggaggagcgg gagatgcaga agctctcgga acagataaac accatggccg
     1081 aagagggact gagggtcatt ggcgtagcca aatccgagtt caggcagaca tccctgcctg
     1141 acgggcagca cgaatttagg ttccggttcc ttgggctcat cggattggca gacccggtac
     1201 gtccgggtgt ggctgaggct atcagagaat gttacggtgc cgggatacgg gtggtaatga
     1261 tcacgggaga ctatccggtc actgcccgga aagtggcagt ccagatagga ttaaagcaga
     1321 tggacgggat agtgacaggc caggagatcg agagcatgga tgattctgag ctgagggaga
     1381 ggatcaggac agtgaatgtc tttgcccgtg ttgtccctga acagaagctg aagatagtgg
     1441 atgcactcaa ggcaaatggt gaggttgtgg ccatgacagg agacggagtg aatgatgcac
     1501 cagccctgaa atctgcccac atcgggattg ctatgggcgg gcgcggcact gacgtggccc
     1561 gcgaggcctc ggccctggtg ctcctggacg ataatttttc ctctatagtc caggcagtga
     1621 ggatgggaag gaggatcttc gataacctga agaaggcgat ggcgtatatc tttgcaatcc
     1681 atatccccat tgccggcatg tcgctgctcc ctgtgctttt tcgctggccc cttgtgcttc
     1741 tgcctgtgca tatcgtcttc cttgagttga tcattgatcc cgcctgttct gtggtgtttg
     1801 aggcagagcc tgaggaagcg catatcatga aacgacagcc acggaatctc ctggagcccc
     1861 tcttcgatgc gaggacagtc acctacagcg tgcttcaggg cgcaagcgtt ctttgtatcg
     1921 tcctggcggt attccttacg gcaatgtacc ggggacaggg cgaagcggat gccaggaccc
     1981 tcagcttcac gacgctcatc atcgccaatc tcgggttgat actgacgaac cgctcgtggt
     2041 cccgtgtcat cctctccaca ttgcgcacac caaacgctgc cttctggtgg gtgctgggag
     2101 gagccctttt cttccttggc atggtactgt atgtcccgtt tatgcagaga ctttttcact
     2161 tccagatgct ccatcccctt gacctgatga tctgcctttt ggcgggtatt atcagtgtgg
     2221 tatggtttga agtgctcaag tttgtccgaa gataccggat gtatcctttg agcactaata
     2281 attaaattga catttccatc taatttgggt agaatgaaca gattagaagt ttttgtcaaa
     2341 taaaaggccg caagaacttt tgacactgcg gtcttttttt tgaagagaga ggaaggagat
     2401 caggcaattg gagatacgag taagcggtaa tgacatcgaa aaggcattga aactcctcaa
     2461 acggcagctc cagaaggacg gcctgttgaa ggaactcaag aagaggcgtt tctatgagaa
     2521 gccttcggtc aagatgaaat caaagcagag agaggcgcaa aagaggagag tgaagacctc
     2581 acggatggca aggcaaaaca ggcctgtcag ctaaccctgg aagggccttt tccccagggg
     2641 ttcaaattac ctcacattct gactaccccc ccattttacc aattttccat tccgcagaat
     2701 aagaaagagg agatgaatga actacaacag attttatgaa ttcggacttt ctgacgaggt
     2761 ccttaaggcg gtttttgaca tgggttttga ggaacccacc cccatacaga agattgctat
     2821 cggccacgcg ctcaggggtc atgacgttat tggtctggcc cagaccggca caggaaagac
     2881 tgctgccttt ggcatcccga t
//
LOCUS       DATKOU010000096         6872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2430138, whole genome shotgun sequence.
ACCESSION   DATKOU010000096 DATKOU010000000
VERSION     DATKOU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430138"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /gene="rplX"
                     /locus_tag="VMU21_04390"
     CDS             <1..188
                     /gene="rplX"
                     /locus_tag="VMU21_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HUO76798.1"
                     /translation="HMKPNRKYAQGGIIEREAPIHMSNVILVCPKCNKSTRIANLLME
                     SGKKVRTCKKCKEVIDQ"
     gene            188..754
                     /gene="rplE"
                     /locus_tag="VMU21_04395"
     CDS             188..754
                     /gene="rplE"
                     /locus_tag="VMU21_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HUO76799.1"
                     /translation="MAPKQKGVTVPRLKEKYKKDVVPALMKEFSYKNIMQVPKVEKVV
                     LNVGLGEAIQNIKLLDSVQKELSIIAGQKSLTTKAKKSIAGFKLRQGMPIGCKVSLRG
                     DRMYEFLDRLVSVALPRIRDFKGVSTRAFDGKGNYSLGIKEQFIFPEIEYDKVDMVHG
                     MDIAVCTSAKTDAEAKALLRLLGMPFRK"
     gene            814..999
                     /locus_tag="VMU21_04400"
     CDS             814..999
                     /locus_tag="VMU21_04400"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HUO76800.1"
                     /translation="MAKKCMIEKVKRTPKFEVRAYNRCRVCGRPRGFLRRFGMCRICF
                     RSLALRGQIPGVTKSSW"
     gene            1010..1402
                     /gene="rpsH"
                     /locus_tag="VMU21_04405"
     CDS             1010..1402
                     /gene="rpsH"
                     /locus_tag="VMU21_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HUO76801.1"
                     /translation="MMTDPIADMLTRIRNAALIRAEKVDVPASRMKLEIAKILKEEGF
                     IRAYKILKDKRQGILRLTLKYVENENVISGLRRVSKPGRRMYVGSKEIPRVMGGVGIA
                     ILTTSQGILSDKSCRRDSLGGEVICYVW"
     gene            1415..1957
                     /gene="rplF"
                     /locus_tag="VMU21_04410"
     CDS             1415..1957
                     /gene="rplF"
                     /locus_tag="VMU21_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HUO76802.1"
                     /translation="MSRIGKKPIDVPGGVDIKLEGQTIRVKGPKGELNWDFPRAITVS
                     LGEGKVLIERASDSKMDRSLHGLSRSLVANMVKGVSQGYQRILEISGVGYRAQVQGSK
                     IALTLGYSHPVDFQLPPGISAAVDQKQTTVTLSGIDKQQLGQVAANIRALRSPDIYKG
                     KGIRYAGERIKLKVGKAGKK"
     gene            1972..2331
                     /gene="rplR"
                     /locus_tag="VMU21_04415"
     CDS             1972..2331
                     /gene="rplR"
                     /locus_tag="VMU21_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HUO76803.1"
                     /translation="MKSITEARIRRHERIRKEVSGTPERPRLSVYRSLNHIYAQIIDD
                     SQGSTLVAASTLDKDFSGEKGHKGNVSMAKKVGELIAKRALEKGLKKVVFDRGGYLYH
                     GRVKALADGAREAGLEF"
     gene            2408..2944
                     /gene="rpsE"
                     /locus_tag="VMU21_04420"
     CDS             2408..2944
                     /gene="rpsE"
                     /locus_tag="VMU21_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HUO76804.1"
                     /translation="MGRIDPEGLNLKDRVVYINRVAKVVKGGRRFSFSALVVVGDGGG
                     TVGIGKGKATEVPEAIRKAVEQAKKSLIKFPVKDGTIPHRIIGKFGSGTVIMNPAVKG
                     TGLIAGGAVRAILEVAGIQDVVTKALGGHNPFNTVKATIDGLVRLKDPHVVSKLRRKT
                     EEETEGQEISEIRRGEKE"
     gene            2952..3395
                     /gene="rplO"
                     /locus_tag="VMU21_04425"
     CDS             2952..3395
                     /gene="rplO"
                     /locus_tag="VMU21_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HUO76805.1"
                     /translation="MRIEELQPAEGSRKGNKRVGRGVGSGLGKTSGKGHKGQKARSGG
                     TKGAGFEGGQMPLQRRIPKRGFTNYPFSKEYAIINLKDLSRIEDLNVITPEALYERGI
                     VKDIKDGLKILGEGEITKPLTVKANAFSETALKKIAGAGGKAEVI"
     gene            3396..4700
                     /gene="secY"
                     /locus_tag="VMU21_04430"
     CDS             3396..4700
                     /gene="secY"
                     /locus_tag="VMU21_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HUO76806.1"
                     /translation="MRVLTGFQNIFKIPELKSRVLFTLALLTVYRIGCHIPTPGINGE
                     ELSKFLTDKGGALMGFFDMFSGGALSRVTIFALGIMPYISASIIFQLLTVVIPAIGKL
                     AKEGEAGRKKIIKYTRYGTVVISAIQSFGIAVGLESMAQGAFIQNPGWSFRLLTMLTL
                     TSGTAFIMWLGEQITERGIGNGISLIIFAGIVARFPNAVASTFRLVQAGELSLFFVIL
                     LVVMMIGVVGAIVFIERGQRKIPVQYAKRVVGRKVYGGQSTHLPLKINTAGVIPPIFA
                     SSIIMFPATVAGFIAVPWVQEVAKQFSPGTTLYTLLYVSMIFFFSYFYTAIVFNPVDI
                     ADNLKKYGGYIPGIRPGQKTSEYIYKVLSRLTFVGAFYLAIVCVLPELLIRKFNVPFY
                     FGGTSLLIVIGVALDTVSQIESHLLTRSYEGFLRKGRIKGRR"
     gene            4710..5459
                     /gene="map"
                     /locus_tag="VMU21_04435"
     CDS             4710..5459
                     /gene="map"
                     /locus_tag="VMU21_04435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HUO76807.1"
                     /translation="MIILKSPDEIERMAKACRIVAETLRALEGMVRPGITTREIEGFV
                     EKMVVAKGAIPAFKGYRNYPSSVCTSVNDQVVHGIPSSLGLKEGDIVSIDLGVFLDGF
                     YGDGAVTFPVGTISPLAERLIKVTEESLALGIERARPGNRISDISHAIQKHVEKSGFS
                     VVRAFVGHGIGRSLHEEPQVPNFGDPGRGPRLREGMTLAIEPMVNEGRYEVSILEDGW
                     TAVTADGTLSAHAEHTIAITRNGPRILTKTD"
     gene            5482..5700
                     /gene="infA"
                     /locus_tag="VMU21_04440"
     CDS             5482..5700
                     /gene="infA"
                     /locus_tag="VMU21_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HUO76808.1"
                     /translation="MPKEENIEVQGTIVETLPNAMFRVELENGKVILAYVSGKMRMHF
                     IKILPGDKVSVELSPYDLTKGRITYRFK"
     gene            5783..5896
                     /gene="rpmJ"
                     /locus_tag="VMU21_04445"
     CDS             5783..5896
                     /gene="rpmJ"
                     /locus_tag="VMU21_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HUO76809.1"
                     /translation="MKVRASVKPICAKCKVVKRKGVIRIICENPRHKQRQG"
     gene            5905..6279
                     /gene="rpsM"
                     /locus_tag="VMU21_04450"
     CDS             5905..6279
                     /gene="rpsM"
                     /locus_tag="VMU21_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HUO76810.1"
                     /translation="MARIAGVDLPKNERIEIGLTRIFGIGRPLSQKILSETGVNPNVR
                     VKDLRDEDIVKIRNVIDRDFKVEGDLRREVSMNVKRLIDIGSYKGQRHRLGLPVHGQR
                     TKTNARTRRGPRKLVVGKKKEA"
     gene            6285..6671
                     /gene="rpsK"
                     /locus_tag="VMU21_04455"
     CDS             6285..6671
                     /gene="rpsK"
                     /locus_tag="VMU21_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HUO76811.1"
                     /translation="MAQRKKTSKKEKKNIHTGSAHIQATFNNTIVSITDQNGNVVAWS
                     SSGSLGFKGSRKGTPYAAQMAAEAAAKKAMDFGMKQLDVFVKGPGAGRESAIRALQAA
                     GLDINLIKDVTPVPHNGCRPPKRRRV"
     gene            6671..>6872
                     /locus_tag="VMU21_04460"
     CDS             6671..>6872
                     /locus_tag="VMU21_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HUO76812.1"
                     /translation="MSRYIGSVCRMCRRAGEKLFFKGDRCFTEKCGVERRKYPPGQHG
                     QSRAKLSDYGVQLMEKQKVRKPY"
BASE COUNT         1943 a         1338 c         2040 g         1551 t
ORIGIN      
        1 ggcatatgaa gcccaatagg aaatacgcac agggaggcat tatcgagagg gaagccccta
       61 ttcacatgtc caatgtcatc cttgtgtgtc ccaagtgtaa taaatctact cgaattgcca
      121 atctgcttat ggagagcggg aaaaaagtga gaacgtgtaa gaaatgtaag gaggtcatag
      181 accagtaatg gctccaaaac aaaagggagt taccgttcca agacttaagg aaaagtacaa
      241 gaaggatgtt gtgcctgcac ttatgaagga gttttcctat aagaatatca tgcaggtgcc
      301 taaggttgaa aaggtggtgc tcaacgttgg tcttggtgag gcgatccaaa atataaaact
      361 cctggattcc gtacagaaag agctctccat cattgcagga cagaagagcc tgaccactaa
      421 ggcaaagaaa tccattgcag ggtttaagct gaggcaggga atgcctatag ggtgcaaggt
      481 ttccctgagg ggtgacagga tgtacgaatt cctcgacagg ttggtcagcg tcgcattgcc
      541 ccgtatcagg gatttcaagg gggtttccac aagggccttt gacggaaaag gcaattattc
      601 tctggggata aaggaacagt tcatattccc cgagattgaa tatgacaagg tagacatggt
      661 acacggaatg gacattgccg tctgtacctc tgcaaagacc gatgcagagg caaaggcctt
      721 gctgaggctt ttaggaatgc ccttcaggaa atgaaacaat aagataacaa ggcgcccata
      781 gagccaagga ctcttgggac aatagaggtg agaatggcta agaaatgtat gatagaaaag
      841 gtgaagagga ctcccaaatt tgaggtgagg gcctataaca ggtgcagggt atgcgggagg
      901 ccgaggggtt tcctcaggag gttcggcatg tgcagaatct gtttcagatc acttgccctc
      961 cggggacaga tccccggggt gacaaagtcg agctggtaga ggtggagaga tgatgacaga
     1021 tcctatcgca gatatgctca cgagaataag gaatgcggcc ctcataaggg cggaaaaggt
     1081 ggatgtccct gcttcgagaa tgaaactcga gatcgcaaag atacttaagg aagagggatt
     1141 cataagggca tataagatat tgaaggacaa gaggcaaggt attctcaggc tgaccctcaa
     1201 atacgtggag aatgaaaatg tgatctcagg gttaagaagg gtgagcaaac caggacgcag
     1261 aatgtatgtg gggagtaagg aaatccccag ggtgatggga ggggttggta tcgccatcct
     1321 caccacatcg caggggatat tgagcgataa atcatgccgt cgggacagtc ttggcgggga
     1381 agtcatctgt tatgtctggt aaggggaatt taagatgtca cggataggaa agaaaccaat
     1441 agatgttccc ggtggtgtgg acattaaatt agagggccag accatcaggg tgaagggacc
     1501 aaagggagag ttaaactggg atttccctag ggcaatcacg gtcagccttg gtgaaggaaa
     1561 agtattgatt gagagggcca gcgattccaa gatggacagg tcgttgcacg gattgtcgag
     1621 aagccttgta gcgaatatgg tgaagggggt ctctcaggga tatcagagga tccttgagat
     1681 atcaggagtg ggatacaggg cacaggtcca ggggagtaaa attgccctca ccctcgggta
     1741 ttcccatcct gttgactttc agctgccgcc agggattagt gccgctgtgg atcagaagca
     1801 gaccaccgtc accctttcag ggatcgacaa gcagcaactg ggacaggttg cggcgaatat
     1861 aagggcattg aggtctcctg atatctataa gggcaagggt atccgttatg ccggcgagag
     1921 gataaaactt aaggtcggta aagcgggcaa aaagtaggcg ggagggattg agtgaaaagc
     1981 ataacggaag cgcggataag aagacatgag agaataagga aagaggtctc cgggactcct
     2041 gagagaccaa ggctttcggt atatagaagt ctgaatcata tctatgccca aattattgat
     2101 gattctcagg ggagcaccct tgtggccgcc tcgactcttg ataaggattt tagtggtgaa
     2161 aagggacaca agggcaatgt gagcatggcg aaaaaagttg gagaactcat tgcgaaaagg
     2221 gcccttgaaa agggtttgaa aaaagttgtg tttgacaggg gtggatatct atatcatggc
     2281 agagtaaagg ctcttgcaga tggagcgaga gaagcggggc tggaattcta gatacttgtg
     2341 agcgttcagg tttcagtttt aggctgaggc tgacaactga ttactaaaga ctgtgtaagg
     2401 agggagagtg ggaagaattg atccagaagg actgaacctg aaggataggg ttgtctacat
     2461 taacagggtc gcaaaagtgg tgaagggagg aagaaggttt tcctttagtg cccttgtggt
     2521 ggtgggtgac ggcggcggta cggtaggcat tggtaaggga aaagcaaccg aggtgcccga
     2581 ggccatacgg aaggcggtag aacaggcgaa aaaatccctc atcaagtttc ctgttaagga
     2641 tggaacaatt ccacaccgga tcatcggaaa gtttggttca gggaccgtca tcatgaaccc
     2701 tgccgtgaaa ggtaccgggc ttatcgccgg aggtgcggtg agggcgatac ttgaagtggc
     2761 ggggatccag gacgtagtta ccaaggctct gggggggcat aacccgttca atacggttaa
     2821 ggcgactatt gacggactcg tgagacttaa ggatccccac gttgtctcta aattgaggag
     2881 aaagacagag gaagagacag agggtcaaga gatatcggag attcgccgag gcgaaaagga
     2941 ataagggaat catgagaata gaagagttgc agccagcgga agggagtaga aaagggaata
     3001 aaagagtcgg acgtggtgtc ggctccggtc tcggcaagac ctccgggaag gggcataaag
     3061 gacagaaggc caggtcagga ggcacaaagg gtgctggatt tgaaggaggg cagatgccgc
     3121 tccagcggag gatcccaaag aggggattta cgaattatcc gttttccaaa gagtatgcaa
     3181 ttataaacct gaaggacctt tcaagaatag aggatctgaa tgtgatcacc cccgaagcgc
     3241 tgtatgaaag gggtatagtg aaggatatca aggatggcct caaaatcctc ggcgaaggtg
     3301 agattaccaa gccactcacg gtaaaagcaa atgccttcag tgaaaccgct ttgaagaaaa
     3361 tagcgggcgc gggtggtaag gcagaggtga tctagttgcg ggtccttacg ggctttcaga
     3421 atatattcaa gatacccgag ctcaagagca gggtattatt tacccttgcg cttctcacag
     3481 tgtacagaat tggatgccat atccccactc ccggcataaa cggagaagaa ctgagcaagt
     3541 tcctcactga caagggcggt gctctcatgg gctttttcga tatgttttcg gggggcgctc
     3601 tctccagggt caccatattt gctctcggga taatgcccta catcagtgct tccatcattt
     3661 tccagctcct taccgttgtt atcccggcta tcggaaagct tgcaaaggaa ggggaggcgg
     3721 ggagaaagaa gatcatcaaa tataccaggt atggtaccgt ggtcataagt gctattcagt
     3781 cttttggtat tgcggtggga ctcgaaagca tggcgcaggg cgccttcata cagaacccgg
     3841 ggtggtcatt ccgattgctc actatgctca ctctcacctc aggtaccgca tttatcatgt
     3901 ggcttgggga acagatcacc gagaggggga tcgggaacgg gatatccctc atcatatttg
     3961 cgggtattgt tgccaggttt cccaatgcag tggcgagcac cttcaggctc gtgcaggcag
     4021 gtgaattgtc ccttttcttc gtgatccttc ttgtggtcat gatgatcggg gttgttggtg
     4081 ccattgtatt cattgagcgt ggccagagaa agatccctgt acaatatgca aagagggtgg
     4141 tgggcaggaa ggtgtacgga ggacagagca cccatttgcc tctgaagatc aataccgcgg
     4201 gagtcatccc ccccatcttt gcctcctcca taatcatgtt ccctgcgacc gttgcaggat
     4261 ttatcgcagt cccatgggtg caggaggtgg caaagcagtt ctcccctggt acgacactct
     4321 atacattgct ttacgtaagc atgatatttt tcttcagtta cttctatacc gctattgtct
     4381 tcaatccggt tgacattgcg gataacctca agaaatacgg agggtatatc cccgggataa
     4441 gacctggaca gaaaacgtcg gaatacatct ataaggtcct ctcgaggtta acctttgtgg
     4501 gtgcgtttta ccttgcgatc gtatgtgttt tgccggagtt actcatacgg aaatttaatg
     4561 ttccctttta ctttggaggg acttctctcc tgatcgtcat cggtgttgct ctggataccg
     4621 tttcacagat agagtctcat ctccttacaa gatcatatga gggcttcctc cgcaaaggaa
     4681 ggattaaagg gaggagatga gaggcctgaa tgatcatcct gaagtctcct gacgagattg
     4741 aaaggatggc taaggcatgc cgtattgtgg ctgagaccct cagggctctg gagggaatgg
     4801 tgaggccggg gattacgaca agggagattg aagggttcgt tgaaaagatg gtggttgcga
     4861 agggtgcgat ccctgccttt aagggataca ggaattaccc ctcaagtgtc tgtacctctg
     4921 tcaatgacca ggttgttcat ggtatccctt cctctctggg gttaaaagag ggagatattg
     4981 taagcataga cctgggtgtg tttcttgacg gattctatgg cgacggtgcg gtcacctttc
     5041 ctgtcggcac gataagccca cttgcggagc ggcttatcaa ggtgaccgag gagtctctcg
     5101 cgcttggcat agaacgggca aggcctggta acaggatatc cgatatctct catgcgattc
     5161 aaaaacatgt agagaaaagt ggcttctctg tggtgagggc ctttgtgggt cacggaatag
     5221 gaaggtcatt gcatgaagag ccccaggttc cgaactttgg tgatccaggt cgtggaccga
     5281 ggctcaggga gggtatgaca ttggcaattg aacctatggt caatgagggt aggtacgaag
     5341 tatccatcct ggaggacgga tggactgcgg ttactgctga cggcaccctt tcggcccacg
     5401 ctgaacatac cattgccatt acaaggaatg gtccgagaat cttgactaaa acagactaaa
     5461 gtctttataa tttaaagtta aatgccaaaa gaagagaata tagaggttca ggggacgatc
     5521 gtagagacac tccccaatgc aatgttcagg gttgaactcg aaaacggaaa ggttatcctt
     5581 gcatatgtat cgggcaaaat gaggatgcac tttataaaaa tattgcccgg ggataaggtg
     5641 tccgtggaac tctctcccta tgatctcacg aagggaagaa ttacctacag gttcaaatag
     5701 gaaaaggcaa agcttacaat tggagtctca tctgaaggta gggcatgaac atttttgaat
     5761 ttcttatagg tggggttaga ctatgaaggt tagggcatca gtaaaaccga tatgtgcgaa
     5821 gtgtaaggta gtgaagagaa aaggggtgat taggattatc tgtgagaacc ccaggcacaa
     5881 acagagacaa ggataggagg agggatggcg aggatcgctg gtgttgattt acccaagaac
     5941 gagaggatag aaatcggtct tacacgaata tttggcatcg gaagaccgct gtctcaaaag
     6001 atactgagcg agacaggggt taaccctaat gtgagagtta aggatctgag agatgaggat
     6061 attgtaaaga tacgaaatgt gatcgacagg gactttaagg tggaaggtga tctcaggcgc
     6121 gaggtgtcta tgaacgtaaa gaggctcatt gacattggtt cgtataaggg ccagaggcat
     6181 cgtctcgggc tgccggtcca cgggcagcgg acaaagacca atgcgcgcac acggagaggc
     6241 cctagaaagc tggttgtggg caagaagaag gaggcataac atttatggcc cagaggaaaa
     6301 agacttcgaa aaaagaaaag aagaatatcc ataccggttc tgcccatatc caggcaacct
     6361 ttaataacac gatcgtgagt attaccgatc agaacggcaa tgtggttgca tggtcgagtt
     6421 caggcagtct cggatttaag ggttcaagga agggaacgcc gtatgccgca cagatggccg
     6481 cagaggctgc tgcgaaaaag gcaatggatt ttggcatgaa gcaactggat gtgttcgtga
     6541 aagggcccgg tgcgggaagg gaatctgcca tcagggcact gcaggctgcc ggtcttgata
     6601 ttaatctcat caaggacgtg acgcctgttc ctcacaatgg gtgccgaccg ccaaagagga
     6661 ggagggtgta gtgtcgagat acataggatc agtatgcagg atgtgccgga gggcagggga
     6721 aaaactcttt ttcaagggtg atagatgttt tactgagaaa tgcggggtag agagaagaaa
     6781 atatcccccg ggccagcatg gacaaagcag ggcaaagctc tctgattacg gcgtgcagtt
     6841 gatggaaaaa cagaaagtga gaaaaccata cg
//
LOCUS       DATKOU010000097         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4358206, whole genome shotgun sequence.
ACCESSION   DATKOU010000097 DATKOU010000000
VERSION     DATKOU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4358206"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VMU21_04465"
     CDS             <1..229
                     /locus_tag="VMU21_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HUO76813.1"
                     /translation="MKGTTGKGEYVVNRGGKYFLDLATANKYQALSMDVPPDNIWMSD
                     ECTFCLTEKYYSYRFAKGPTGRQGGFIRILP"
     gene            387..845
                     /locus_tag="VMU21_04470"
     CDS             387..845
                     /locus_tag="VMU21_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO76814.1"
                     /translation="MLKAKDIMTKDVITLSPDTTVEKAGRLFIDRGMSGAPVVDSEGK
                     LFGVVTENDLISKNRRLHIPTVLRLFDAFIPLGASKLEEELKRMTASTVGEICSRDVV
                     TVDEEASIEDIATIMTERNVHLLPVMGKGKLMGIIGKKNLIRGIAGETSQ"
     gene            829..1278
                     /gene="tsaE"
                     /locus_tag="VMU21_04475"
     CDS             829..1278
                     /gene="tsaE"
                     /locus_tag="VMU21_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HUO76815.1"
                     /translation="MRLLSNGPGETFQYGLKLGRLLHAGDIVCLFGDLGSGKTTLVKG
                     IASALAIPERDITSASFTIIAVYKGTLRHSSVPFYHIDLYRIKDATDLDSIGIEEYIG
                     GDGITVIEWAERMGKMEDSITVRLSILNAEKREIAIEGIDEKDWNNL"
     gene            1253..2116
                     /locus_tag="VMU21_04480"
     CDS             1253..2116
                     /locus_tag="VMU21_04480"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HUO76816.1"
                     /translation="MKRIGIICKPGMTASGEILGDLLPWLKRRGIQVFLDAEVAQPLG
                     IEGYPRSEIPDLTEGVVVFGGDGSMLSAARLVCEKGIPILGVNLGGLGFITEVHAAGI
                     YEAMEHVISDRFTVEERLMLTASVYRKKEKVANYTVLNDVVINKGALARIIDLETCVD
                     KTYVTTFKADGLIVSTPTGSTAYSLSAGGPILYPTTGCIVLTPICPHTLTNRPIVLPD
                     KAVVEISLKSVAEDVFLTMDGQVGFSLKQRDRVVVEKSPFKTRLLIPDESDYFKVLRT
                     KLGWGERGRKA"
     gene            2139..>2828
                     /locus_tag="VMU21_04485"
     CDS             2139..>2828
                     /locus_tag="VMU21_04485"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HUO76817.1"
                     /translation="MDIGQDIKTILNFFQSLGIERLPIEIPTGKDLLAVTKSKDSEFK
                     APEPRTLNPELSSSHDKKAALKKLREEIGECQRCKLSKERKHIVFGEGSPDAGLMFIG
                     EAPGKEEDIQARPFVGDAGKLLTRMIEKMGLAREDVYIGNIVKCRPPLNRDPEEDEMK
                     TCSPFIDRQIEIISPKVIVSLGRVSAQTLIATKIPISKLRGRFYEYKGIPLMPTFHPA
                     YLLRNPKDKWLV"
BASE COUNT          754 a          647 c          788 g          639 t
ORIGIN      
        1 aatgaaaggg acaacaggga agggtgaata tgtggtgaac aggggcggga aatattttct
       61 cgacctcgcc actgcgaaca agtatcaggc tttgtccatg gacgtccccc cagataatat
      121 atggatgtct gatgaatgca ccttctgctt gacagaaaaa tattattctt acaggttcgc
      181 caaaggcccc acagggagac aggggggatt catcaggatc cttccgtaga atatccttgg
      241 cggtgaatga ttcgtgcttt tctcacttac ccttgaacgc gggctttgcc tttttccggg
      301 gcatctttgt tcttgtatga tttaccatag ttttcttata atgagagaaa aacggataac
      361 tactctcgct gtgtggagga gccattatgc tgaaagcaaa agatatcatg acaaaggacg
      421 tgatcacact ctctccggat accacggtgg aaaaagcggg gagacttttt atcgacaggg
      481 ggatgagcgg cgctccggtg gttgattctg aggggaaact tttcggtgtt gtcacggaaa
      541 atgatcttat cagcaagaac aggaggctcc atatccccac cgtgttaagg ctttttgacg
      601 cattcatacc tctgggcgcc agcaagctgg aggaagagct taagaggatg actgcatcca
      661 cagtaggcga gatatgctca agggatgtgg tgaccgtgga cgaagaggcg tccatagagg
      721 atatcgctac cataatgacc gagaggaatg tccatcttct gcctgttatg gggaagggaa
      781 aattgatggg gatcatcggc aagaaaaacc tgatcagggg cattgctggt gagacttctc
      841 agtaacgggc ccggagaaac cttccagtac ggcctcaagc tcggaaggct tctccatgcc
      901 ggagacattg tctgcctttt tggagacctg ggttccggta aaacgaccct ggtcaagggg
      961 attgcgtctg cactcgccat tcctgaaagg gacatcacca gcgcaagttt cacgattatc
     1021 gcagtatata agggcacatt gcgccacagt tcagtcccct tttaccatat tgacctttac
     1081 aggataaagg atgcgacaga ccttgattca atcggcatag aagagtatat tggaggggac
     1141 gggatcacgg tcatagaatg ggcggagaga atggggaaaa tggaagactc cattacggtg
     1201 agactgagca tcctgaacgc ggaaaagcgg gagattgcaa tcgagggaat agatgaaaag
     1261 gattggaata atctgtaagc cgggaatgac cgcatccgga gagatcctgg gagaccttct
     1321 gccctggctt aagaggaggg gcatccaggt ctttcttgat gctgaggtgg cccaaccact
     1381 tggcatcgag gggtatcccc gctccgagat tcctgacctc accgagggcg tggttgtgtt
     1441 cggcggggat ggttcgatgc tctctgcggc tcgcttggtg tgcgaaaaag ggattcccat
     1501 actgggcgta aaccttggcg gcctcgggtt catcaccgaa gtgcatgcag cgggcatcta
     1561 tgaggccatg gagcatgtta tttcggacag atttacggtg gaagagaggc ttatgctcac
     1621 cgcttccgta tacaggaaga aggaaaaagt cgcgaactat acggtgctta atgatgtggt
     1681 cattaataag ggcgcccttg caaggatcat tgatctcgag acatgtgtgg acaagacgta
     1741 tgtcaccacg ttcaaggcgg acggtctgat cgtatcaaca cctacgggct ccactgcgta
     1801 cagtctttcg gcaggaggcc cgatacttta tcccacaaca ggctgcattg tgcttacccc
     1861 catatgtccc cacacactca caaacaggcc catcgtgctg cctgacaagg cggtggtgga
     1921 gatttcgctc aaatctgttg cagaggatgt gttcctcacc atggacggcc aggtgggttt
     1981 ttctttgaaa cagcgcgaca gggtggtggt cgaaaagtcg ccctttaaga cgaggctcct
     2041 gatccctgat gagagcgatt atttcaaggt cctgaggaca aaactgggat ggggggagag
     2101 gggcagaaag gcataatcgc ggtgcggtcg aagatcgaat ggatatagga caggacataa
     2161 aaaccatact taatttcttt caatccctcg ggattgaaag gctgcccatt gaaattccaa
     2221 cgggtaagga tttactggcc gtgaccaaaa gcaaagattc ggaattcaaa gccccagaac
     2281 ccagaactct gaacccagaa cttagctctt cacatgataa gaaggccgcc cttaaaaagc
     2341 tgcgtgaaga gatcggggaa tgccaacgat gcaagctttc caaggaaagg aaacacatcg
     2401 tgttcggcga aggcagccct gatgcggggt taatgttcat cggcgaggcc ccgggaaaag
     2461 aggaagacat tcaggcaagg ccattcgtgg gagatgcggg caagctcctc acgcgtatga
     2521 tcgagaaaat gggtcttgca cgggaagatg tctatatagg aaatatcgtg aaatgcaggc
     2581 ctcccctcaa ccgcgatcct gaggaagacg agatgaagac atgcagtccg ttcatcgaca
     2641 gacagataga gatcatttct cccaaggtca ttgtatcact gggtcgcgtc tctgcccaga
     2701 cgcttattgc cacaaagatt cccatatcca aattgagagg gaggttttac gaatataaag
     2761 gcataccgct catgcccacc ttccatcccg catacctcct cagaaatccc aaggacaaat
     2821 ggctcgtg
//
LOCUS       DATKOU010000098         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3204597, whole genome shotgun sequence.
ACCESSION   DATKOU010000098 DATKOU010000000
VERSION     DATKOU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3204597"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /gene="mutS"
                     /locus_tag="VMU21_04490"
     CDS             complement(<1..1609)
                     /gene="mutS"
                     /locus_tag="VMU21_04490"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HUO76818.1"
                     /translation="MFRLGDFYEMFGEDAKVASKILQIALTSREKGKEEPIPMCGIPF
                     FAAESYIAKLVKAGHKVAVCEQMEDVKEAKGIVERDVVKVITPGTYTPENPKENNYIF
                     SFMPEGRKHGVALVDISTGEFMVYETEKPIEDELSRFEPKELLVPESLKANLHYAEIL
                     KSYYTTAIDDWSFDFSEAYRTLLKHFGVSTLDGYGCEGMHTAISAAGALLAYIEDTQK
                     EAIRFKKISPLTQSAYMFLDAATQKNLELIRNVKGGTEGTLLSVLDETLTPMGGRYLR
                     NAILRPLVDAAEIGNRLGAVEVLLDDYELVDMQRSNLRKIQDLERLASKVDNRSANPR
                     DLIAIKHSLSCLPAIKKVLVASHNHSLRSLGEALPDFQALGALIDSAILENPPLSVRD
                     GSILKDGYHPEVDGLRKVSVSGKDFIAELEARERKKTGITSLKVGYNRVFGYYIEVTK
                     TNLDLVPDYYVRKQTLVNGERFITPELKEYESRVLGSEERLKDLEYSLFQEILEKVQG
                     EGTRLVSTGSIIAKIDFLISLAVVARRH"
     gene            complement(2001..2378)
                     /locus_tag="VMU21_04495"
     CDS             complement(2001..2378)
                     /locus_tag="VMU21_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HUO76819.1"
                     /translation="MEEKKFVFILTHSYDRPDIVAGAMQLASNMKAFDIELDFCLMDD
                     GVLVAKKGFADTLSELQKKDEFSPVHQLMRTLIDDFGVKFYICASCVKHYGLDKADLV
                     ETAEIKPGSFLGEMLMERQNVTF"
     gene            complement(2461..2535)
                     /locus_tag="VMU21_04500"
     tRNA            complement(2461..2535)
                     /locus_tag="VMU21_04500"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2501..2503),aa:Gly,seq:ccc)
BASE COUNT          740 a          788 c          664 g          792 t
ORIGIN      
        1 tatgtctcct cgcaacaacc gcaagggaga taaggaaatc gatctttgcg atgatactcc
       61 cggtagatac caaacgagtg ccttccccct gaaccttttc caggatttcc tggaataggc
      121 tatattcaag atccttcaaa cgttcctctg aaccgagcac ccttgactca tattccttga
      181 gttccggggt gatgaatcgt tctccgttaa cgagggtctg ttttcttaca taataatccg
      241 gtacaagatc aaggtttgtc ttggtgacct caatgtaata gccaaatacc cggttatatc
      301 ccactttgag agaggtgatg ccggtttttt ttctttccct cgcctccagc tcagcaatga
      361 aatcctttcc gctcaccgac acctttctga gcccatccac ctccgggtga tatccgtctt
      421 tcagtatgct cccgtccctg acgctgagag ggggattctc caggatcgca gaatctatca
      481 gcgcccctag cgcttggaag tccgggagtg cttccccaag tgatctgagt gagtgattgt
      541 gcgaagcgac gagtaccttc tttattgcgg gcaggcatga gagcgagtgc ttgattgcga
      601 tcaagtccct tgggttagct gaacggttgt ccacctttga tgccagcctt tccagatcct
      661 gtatcttcct gagattgctt cgttgcatgt ccacgagttc ataatcgtca aggagaacct
      721 ccactgctcc aaggcgattc cctatttcag cggcatccac aaggggtctt aggattgcat
      781 tcctcaaata cctccctccc atgggggtga gggtttcgtc gaggacagag agaagggttc
      841 cctcagtccc tcccttcaca ttccgtatca gctcgagatt cttctgggtg gctgcgtcca
      901 gaaacatata tgcactctgg gtaaggggag aaatcttctt gaaccgaatg gcctcctttt
      961 gggtgtcttc aatataggca agaagggcgc ctgcagctga gatggccgtg tgcatgccct
     1021 cacatccgta tccgtcaagg gttgatacgc cgaagtgttt caggagagtc cggtacgctt
     1081 ccgaaaagtc aaaagaccag tcgtcaatgg cggtggtgta atagctctta agtatctcag
     1141 cataatggag gttcgcctta aggctctccg ggacaagaag ttcttttggt tcaaacctgc
     1201 tgagttcatc ctcaatgggc ttctctgttt cgtagaccat gaattcccct gtggaaatat
     1261 ccacaagggc gaccccgtgt ttcctcccct ccggcatgaa cgaaaagata tagttattct
     1321 ccttgggatt ctccggggta taggtgccgg gggtaataac cttcaccaca tccctctcta
     1381 cgatgccttt cgcctccttt acgtcctcca tctgttcaca tacagcgact ttatgcccag
     1441 ctttcacgag cttcgcgata tacgactcag cggcaaagaa ggggataccg cacatgggaa
     1501 tcggctcttc cttacccttt tccctcgagg tgagcgcgat ctggagtatc tttgatgcga
     1561 cctttgcatc ctctccgaac atctcgtaaa agtcacccag gcggaacatc aggattgcat
     1621 ccttatgtct ctccttaata ccgaaatatt gtttcattaa aggggttaac tcaggcatag
     1681 gatattaaat accatacgat gtcgaaagtg caatagagga tgataatccc tgcgcgcctt
     1741 gaaattcata cgatcatgtc atttcatatc atgtagctca gcacatgaac cggggatgac
     1801 aggcgagtca tttcagagcc aatcaggttt tataaagaac aaagaggagc cacttgcact
     1861 acgtgcgcca atggtcatcg accatggaca gagaagaatg agtagcttat tcgggtaact
     1921 gagatctctt gagcagatga gacacgaaat ttgattgtgt atcaaggtct ggaagaggat
     1981 gccctcaatt catagagcgt ctagaatgtc acgttctgcc tttccataag catttctccc
     2041 agaaatgaac ccggctttat ctctgcggtc tccaccaggt ccgctttgtc cagcccgtaa
     2101 tgttttacac atgaagcaca gatgtagaac ttcacaccga aatcgtctat aagggtcctc
     2161 atcagctgat gcacagggga gaactcatcc tttttctgga gttcagacaa tgtgtccgcg
     2221 aaacccttct ttgcaacgag aaccccgtcg tccataagac aaaaatcgag ttcaatgtca
     2281 aaggccttca tatttgatgc gagctgcatc gcccctgcaa cgatatccgg cctgtcgtac
     2341 gaatgggtca gaatgaatac aaatttcttc tcttccatga ttttcctcct tctatctgtt
     2401 tctcatttca tgccgcatga gacagacaaa acaattctct tcatatcgac caaacaggac
     2461 tggagcgggc gacgggattc gaacccgcga ccttcagctt gggaagctga cactctacca
     2521 ctgagttacg cccgcatcat attgtttata aaccagtgtt cctccaaaag tcaagaaaga
     2581 ccctgacaat gacggttttt cccctgcccg agcagcgtcc ggcccgaagc aagataaaat
     2641 agccctctgc ggcttttccg atattgacta aataaccacc gtctgtcaca acatttccgc
     2701 ttatgtggcc ggatcagtgg ccacccactg agtccgatta tcttggctca gaccggacgc
     2761 tcctttcata atacttaaaa ctcaataacc attcgcaagt agatttgaat cccgatatgc
     2821 ctgttttaac tttttgccag gatattttgc acccctacgc ggatgagcat gaccgcgata
     2881 gatgcaagga gcaacgccat gatcttcgag acggccatta ttcccccacg tccgaaaatc
     2941 ttcagaattc tcgacgaggc cctgaacgaa agccaaacaa taaa
//
LOCUS       DATKOU010000099        11688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4746512, whole genome shotgun sequence.
ACCESSION   DATKOU010000099 DATKOU010000000
VERSION     DATKOU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4746512"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2883
                     /gene="nuoF"
                     /locus_tag="VMU21_04505"
     CDS             <1..2883
                     /gene="nuoF"
                     /locus_tag="VMU21_04505"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HUO76820.1"
                     /translation="YQYVKPEMVRRIVNEHIVGEKPIAEWLAGDDFENFYKKQVKVVL
                     SDCGKIDYESIDSYISSGGYKASEKVFTSMTPEEVIAEMKKSGLRGRGGAGFPTGVKW
                     EACRKVPSDQKYIICNADEGDPGAFMDRAVIEGNPHVVIEGMLIGAYAIGASKGYVYI
                     RAEYPLAVERLTHAIRDALERGYLGKNVFNSGFKFDIRLKLGAGAFVCGEETALIASI
                     EGQRGMPRPKPPFPVHQGLWGKPTVINNVETLANVPYILNRGADWYSSYGTEKSKGTK
                     VFALTGKVKNTGLIEIPMGITLREIIYDIGGGIENGKALKAVQTGGPSGGCIPASMVD
                     IHVDYESLAQVGSIVGSGGMIVLDEDDCMVNMAKYFLQFTQTESCGKCVPCRIGTKRL
                     LEIQERITRGEGKEEDLELLQKLGNSVKAASLCGLGQTAPNPVLSTLRYFKEEYEAHI
                     KFKRCPSLVCREIISSSCQHTCPIGTEASVYTALIAHGRFDEAAQVIRKDNPLASVCG
                     RICHHPCELKCKAGEGGKPIAIRALKRFAVDYAHKKGIQVQIPKDGVNNQKVAIVGSG
                     PAGLTAGYFLALKGYDVTIFEAKPVVGGMLRVAIPEYRLPREVFDIDIAFMESAGVKF
                     RTDTALGRDISVDSLFEEGYKAIFIAIGSHKSMKLGISEEDTEGVIPSLRFLEALHLC
                     KETHPGKRVIAVGGGNSAVDSARAAHRIEGVEEVTILYRRTRSEMPAYKEEVDAALEE
                     GIDIQFLTAPTGILTKGGKLSGVTCIRMKLGNVDKSGRARPIPIEGSEFTLEADTLIV
                     AIGEQPDTSCVDGTSGLEISTTNTIVVDQESLATSREGVFAGGDAVTGPNTVIHAMQA
                     GKRAVESIDRYLRGERSERSYEVTRPSRYVEPVELTDEELIEADRPEMPLLSPEERKK
                     NFEEVELGFTEEMALKEARRCLRCELSTLDGQKAIQEMNLKKRKHS"
     gene            2901..5561
                     /gene="fdhF"
                     /locus_tag="VMU21_04510"
     CDS             2901..5561
                     /gene="fdhF"
                     /locus_tag="VMU21_04510"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3939..3941,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HUO76821.1"
                     /translation="MVKLTINGKAVSVPEGITVLDAAKRFHIPIPTLCHHPKLTPFGG
                     CRLCIVEIKGIPRPVTSCTTPVAEGMEVTTTSDHLEQIRRTVLELILSDHPNDCMVCE
                     RAGDCALQELAYFYEIRENSFEGERRRYTKRDENPFIERDMEKCILCSRCVKVCDEVE
                     GVGAIDFAYRGFKSKICPPYEKALDCEFCGQCVAVCPTGALTGKAWKQKGRQKDIREI
                     DTTCPYCGCGCNLTLHVQGNEVIRVSSRETSLNEGWLCVKGRFGYGFIHSPERLQKPL
                     IRINGTLTESSWDEALDYVAEQLRMIKEKHGPDAIAGLSSAKCTNEENYLFQKLIRAA
                     IGTNNVDHCARYUHSSTVAGLATVFGSGAMTNSMTEIINNDVIFVIGSNTTETHPIIG
                     LKMKKAVQRGAKLIVADPRRIDLVRFANHWLQQKPGSDVALLNSMMHVILREGLEDRE
                     FINAWTEDFEAFRGSLAEFTPETGERISGVPKDKIIKAALLYGNADHAGIYYTMGMTQ
                     HTHGTDNVFSIANLALLTGNLGKESAGVNPLRGQNNVQGATDMGCIPNQYPGYQKVDI
                     PQIKEKFEKEWGLRLSDRIGITATEMTDAALNGSLKAMYVMGENPVLSDPDVNHTTRA
                     LKSLDFLVVQDIFLTETAELAHVVLPAASFAEKEGTFTNTERRVQRVRKAVNPPGEAR
                     VDSLILSDLSARLGYKMDAASADEMFLEIGRVWPSVAGMTYSRLEKRGIQWPCPTIEH
                     PGTPYLFKGGFPRGKGRFTPVKYRPSAELPDSEYPFILSTGRQLFQYHTGTMTRKTTA
                     IEVVSPPYVEINPEDAKMLSLANGQKVKVSSRRGSIEITVRVSERPLKGVVFIPFHFK
                     EAAANVLTNTALDPVCKIPELKVCAVKIEA"
     gene            6044..6658
                     /locus_tag="VMU21_04515"
     CDS             6044..6658
                     /locus_tag="VMU21_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76822.1"
                     /translation="MNGNIDFHIPDCNQFRNGYEAYTRERRGAVYFEDLSLISENWGD
                     PVLMAQGVQRLIRSWHFFFANFDFDSLVNCIDNNFQDLNEFRARNVVSLADADDVRIE
                     SLFNHFLDALKRTRDNAKSPVSVAKALSPLATHFFPLWDSNIAFSYNCFYLADISAPP
                     YIRFCKKMKIMSEHVQHCVSNKDDRSLLKRIDEYNYSKYTMHWI"
     gene            6618..7145
                     /locus_tag="VMU21_04520"
     CDS             6618..7145
                     /locus_tag="VMU21_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76823.1"
                     /translation="MNIIIQNIQCIGYEKKCQAKQLPDTVQTAKAQPSMHRLCRRRYL
                     LIEDRTGKRVNKMKVYSVDKKTQEKMLSWGRYLFWAEILCQRFKDFIDSPHDIENSVD
                     SWTFFALMSQWYASLWVVIEGWQKLGFDDHAINDLIQQRSVFCDLMKRYRNGVFHYQP
                     RMIEERFLAFLREGD"
     gene            7647..7871
                     /locus_tag="VMU21_04525"
     CDS             7647..7871
                     /locus_tag="VMU21_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76824.1"
                     /translation="MKQIKLIVEKHPDGYVAYPLGLKGVVVGQGDTYEEALADVRSAI
                     HFHIESFGEEVFDVDPPILEAFVAEAGVEV"
     gene            7920..8192
                     /locus_tag="VMU21_04530"
     CDS             7920..8192
                     /locus_tag="VMU21_04530"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76825.1"
                     /translation="MAIRYILSEYVEQAMAQAVYDKLEDGTFAGRISACKGVLAFGPT
                     LRQCEEDLRSTLEDWILVGLKLGHPLPVIGEIDLNKEHSREPVDSV"
     gene            8405..8785
                     /locus_tag="VMU21_04535"
     CDS             8405..8785
                     /locus_tag="VMU21_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76826.1"
                     /translation="MGILKRRLPAILGIIVGFLSIMAGSKILLGISSPNYIVLHWLVI
                     YNVSFGVVSVLAGAGLWRLRPWAISLAGLTAASHGFVLALLIVFFISGKPVAYQSILA
                     MLFRTTAWLGIFLFVRTPKGDSSI"
     gene            complement(8838..9143)
                     /locus_tag="VMU21_04540"
     CDS             complement(8838..9143)
                     /locus_tag="VMU21_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO76827.1"
                     /translation="MESKYALRYLPVAVDDLISIFDWIANDSPANADAFIKKLDKQLL
                     NLKNHPFLGRIPRDEKLKDFGYRVLIIEAYLVFYIVRNRTVEIHRVIHGSRNLDNII"
     gene            complement(9133..9402)
                     /locus_tag="VMU21_04545"
     CDS             complement(9133..9402)
                     /locus_tag="VMU21_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HUO76828.1"
                     /translation="MPIIKSISSLRNRTRDIASLCHEQDEPVYLTTNGEGDLVVMSIE
                     HYERLKARIDLFEKLAVAQAQAAAGTKGYTHSQVMSKLRQRTHGK"
     gene            complement(9655..11103)
                     /locus_tag="VMU21_04550"
     CDS             complement(9655..11103)
                     /locus_tag="VMU21_04550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HUO76829.1"
                     /translation="MPDIGPAMPEIVMAVLAMAVLLLDLMVKRKASIALLSILTAGIV
                     AYFVMASSGVTFSGMYIADSYSLFFKLIFLISLVLSILISIRYIELEKLNFGENYRLE
                     KVNFGEYYSLMLFSTLGMMVMASAGDIIVLFLGLELMSLSLYVLAGFFRHDTKSNEAS
                     LKYFLLGAFASAFLLYAMSLIYGMTGTTNLNAIAKYIAANGIGHNSVTMLSMMLFVVA
                     FGFKIAAAPFHMWAPDVYEGSPTSITAFMSVGPKAAGFAALGRVFFMAFGDLQIDWAG
                     IIIPIAILTMAVGNILALSQTNIKRMLAYSSIAHAGYALIGIIAGGTEGVASMMNYLF
                     IYAFMNIGAFAIVIMLRTEGFKGEEISDYEGLAKSHPLASALMLIFMFSLTGIPPTAG
                     FVGKFYLFMAAVNAGYTWLAIVAVIFSSISAYFYLRIVMNMYMKEPRQVVKLSTSLAT
                     NLALTVTILAVLLIGVLPAGVISMARAAVMGF"
     gene            complement(11159..>11688)
                     /locus_tag="VMU21_04555"
     CDS             complement(11159..>11688)
                     /locus_tag="VMU21_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HUO76830.1"
                     /translation="ILQMLNHGIVTGGLFLSVGVVYDRTHSREIRDYGGLATVLPLYA
                     AFFMVFTLASIGLPGMNGFIGEFLIILGGFTASKWAGVLSALGIIIGAGYMLWLYQRV
                     FFMETNQKLIGLQEMDTREIITLLPMVILIFWIGVYPDTFLSFMHPSVEHLLQRLHSG
                     GQEMTAVAKIMEIIK"
BASE COUNT         3229 a         2588 c         3008 g         2863 t
ORIGIN      
        1 tatcaatatg ttaaacccga aatggtccgc cgcattgtga atgaacatat tgttggagag
       61 aaaccgattg ctgagtggct tgcaggggat gacttcgaga acttctataa gaagcaggtg
      121 aaggtagtcc tgtcggactg cggaaagatc gactatgaga gtatagactc ttatatctct
      181 tccggaggct acaaagcatc agaaaaggtc tttacttcca tgacccctga agaggtaatt
      241 gcagagatga aaaaatcagg gctcagggga cggggaggtg ctggtttccc cacaggggtc
      301 aaatgggagg catgcaggaa ggttccctct gatcagaaat acataatatg caacgccgat
      361 gagggagacc cgggggcatt catggacagg gcagtgatag agggcaatcc ccatgtcgtt
      421 attgagggga tgttaatagg cgcctatgcc attggtgcaa gcaagggata cgtatatatc
      481 agggcagaat accccctcgc cgtggaaagg cttacccatg ccataaggga tgccctggaa
      541 agaggatacc tcggaaagaa cgtctttaac tccggattca agtttgatat acggttgaag
      601 ctcggtgccg gagcttttgt atgcggagaa gagactgccc tcatcgcatc catcgaggga
      661 cagaggggaa tgccgaggcc gaagccgccc ttccctgtgc atcagggatt atggggaaag
      721 cccactgtta ttaataatgt agagaccctt gcaaatgtcc cgtatatcct caacagggga
      781 gctgactggt actcatccta cggcactgag aagtccaaag gaacaaaggt cttcgcactc
      841 acgggaaagg tgaagaacac aggtcttatc gaaatcccga tggggattac cctgagagag
      901 ataatttatg atatcggcgg cggtatagaa aacggcaagg cactaaaggc tgtccagacg
      961 ggagggcctt ccggaggctg catacctgcc agtatggtgg acatccatgt tgattatgaa
     1021 tcccttgccc aggtcggttc gattgtcggc tcgggaggaa tgatcgtact tgacgaagat
     1081 gactgcatgg tgaatatggc aaagtacttt cttcagttca cacagactga atcgtgcgga
     1141 aaatgtgtgc cttgcagaat aggaacaaag agacttctcg aaatacagga aaggatcacg
     1201 aggggagagg gcaaggaaga agatcttgaa ctcctccaga aattgggcaa tagcgtgaag
     1261 gccgcttccc tgtgcggtct tggccagacc gctcccaatc ccgtcctcag caccctcagg
     1321 tatttcaagg aagagtacga agctcacatt aaatttaaac ggtgtccttc actcgtctgc
     1381 agggagataa tatcttcatc ctgtcagcac acctgtccta tcggaaccga ggcatctgtc
     1441 tacaccgctt taattgccca cggacgtttt gatgaggccg ctcaagtgat cagaaaggat
     1501 aatccgctgg caagcgtctg tgggaggatt tgccatcatc cctgtgaatt aaagtgcaag
     1561 gcaggtgaag gagggaaacc gatcgccata agggcactga agcggtttgc cgttgactat
     1621 gcgcacaaaa aaggcattca ggtgcagatc ccgaaggacg gagtgaacaa tcaaaaggtc
     1681 gccattgtgg gttctggacc ggcagggctt accgcaggtt atttcctcgc tctaaaggga
     1741 tatgatgtca cgatctttga ggcgaagcct gttgtcggtg gaatgctcag agtcgcgata
     1801 cctgaatacc gcttgccccg cgaggtcttt gatatcgaca tcgctttcat ggagagcgct
     1861 ggagtaaagt ttaggacaga cactgcgctg gggagagata tttccgttga cagccttttc
     1921 gaggagggat ataaggccat atttatcgcg atcggttccc ataagtcgat gaaactcggt
     1981 atttcagaag aagatacgga aggtgtcata ccctccctga gattcctcga agcgttgcat
     2041 ctctgtaaag agacgcaccc cggcaagagg gtcatagcgg ttggaggcgg caactcggcg
     2101 gtagattctg cgagggccgc gcatcgtatt gagggggtag aggaggtgac gatactgtat
     2161 aggaggacgc ggtcggagat gcccgcatat aaagaggaag tcgatgctgc actggaagag
     2221 ggtatcgaca tacaattcct gactgcgccc actgggattc tcaccaaggg tggtaaattg
     2281 agtggtgtaa cctgtattcg catgaaatta gggaatgttg ataaaagtgg aagggccagg
     2341 cccattccga tcgagggttc tgagtttact cttgaggcag atacattgat agtggcgatt
     2401 ggcgagcagc ctgatacatc ctgcgtcgac ggaacctcag ggcttgagat ctctacgacg
     2461 aacaccatcg tcgttgatca ggagagcctg gcgacatcca gggaaggtgt ttttgccggc
     2521 ggcgatgccg tgacaggtcc gaatacggtt atccatgcta tgcaggcggg aaagagggca
     2581 gttgagtcga tcgacagata ccttcgggga gaacgttcag aaaggagcta tgaggtgacg
     2641 agaccctccc ggtatgtcga accggtcgaa ctgactgatg aggagctcat agaagcagac
     2701 cgtcctgaga tgccgctcct ttctcctgag gagaggaaga aaaatttcga agaagtagag
     2761 ttaggtttta ccgaagagat ggcgctcaaa gaggcgaggc ggtgtctcag atgcgaattg
     2821 agcactctgg atgggcaaaa ggcgatccag gaaatgaacc taaagaaaag aaagcattca
     2881 taaggaagtc aggaattgat atggtgaaac tcacaataaa tggcaaggca gtttctgttc
     2941 cggaaggaat aacggtcctg gatgccgcga aaagattcca tatccccata cccacccttt
     3001 gtcatcatcc aaaacttacc ccttttggcg gctgtaggct ctgcatcgtc gagatcaagg
     3061 gtatccccag acccgtgact tcatgcacca ccccggtagc tgagggtatg gaggtgacca
     3121 ccaccagtga ccatcttgaa cagataagga ggaccgtcct ggaactcatc ctctctgacc
     3181 accccaacga ctgcatggta tgtgaacgtg cgggtgattg cgcactccaa gaactcgcgt
     3241 atttttacga aataagggaa aacagctttg aaggagagag aaggagatac accaaaaggg
     3301 atgagaatcc ctttatcgaa agggacatgg agaaatgcat cctttgcagc agatgtgtga
     3361 aagtgtgcga tgaggttgag ggggtgggag ccatcgattt tgcatacaga ggctttaagt
     3421 caaaaatatg tcctccttat gaaaaagccc ttgattgcga gttctgcggt cagtgcgttg
     3481 cggtctgtcc cacgggggct ctcaccggca aggcctggaa gcagaaggga agacagaaag
     3541 acataagaga gatagacacc acctgcccct attgcggctg tggctgtaac cttacgcttc
     3601 atgttcaggg gaatgaggtg atcagggtca gttcccggga aactagcctc aatgaaggat
     3661 ggctttgtgt gaaagggagg ttcgggtacg gtttcataca cagccctgaa cgcctgcaaa
     3721 aacctcttat acggataaat ggaacactga ctgaatcgtc gtgggatgag gcccttgatt
     3781 atgtagccga acaattgaga atgatcaaag agaaacacgg ccctgacgcc atagccggac
     3841 tgtcttccgc aaaatgcacc aatgaagaga actacctctt ccagaaactc ataagggctg
     3901 ccataggaac aaacaatgtg gaccactgtg cccggtactg acatagttca acggtggccg
     3961 gtctggccac ggtattcggc tcgggagcca tgacaaattc catgacagag attataaaca
     4021 atgatgtgat ttttgtcata ggttccaaca ccaccgaaac ccaccccata atcggactaa
     4081 agatgaagaa ggcggttcaa agaggagcca agctcattgt tgcagatccg aggaggatag
     4141 atcttgtgag gttcgccaat cactggctcc agcaaaagcc tggctctgat gttgcccttc
     4201 tcaactccat gatgcatgtg atcctcaggg aaggcctaga agacagggag ttcatcaacg
     4261 cctggacaga ggactttgag gcattcaggg gctccctggc tgaattcacg cctgagacag
     4321 gggaaaggat atcgggagtt cccaaggaca agatcataaa ggcagccctg ctttacggaa
     4381 atgccgacca tgcggggata tattatacca tgggtatgac acaacacacc catggcactg
     4441 acaacgtatt ttcaatagct aacctcgctc tcttgactgg aaatctgggg aaggaaagtg
     4501 caggggtgaa tccgttgaga ggtcaaaaca atgtccaggg tgctacggat atggggtgta
     4561 tacccaacca gtaccctggt taccaaaagg ttgatattcc ccagataaaa gagaaatttg
     4621 aaaaagagtg ggggctgagg ctctctgaca ggatcggcat caccgccaca gagatgacgg
     4681 acgctgcact taatggtagc ctcaaggcca tgtatgtcat gggagaaaac cctgtactga
     4741 gcgacccgga cgtgaaccac actacaaggg cattgaaaag tctggatttt cttgtagtgc
     4801 aggacatatt ccttacagaa accgcagaac ttgcccatgt ggtgcttccg gctgcatcat
     4861 tcgcagagaa agagggcacc tttaccaata ctgagcgacg ggttcagcgc gtgagaaagg
     4921 cggtgaatcc tccaggagaa gcgagggttg actcccttat tctgtctgat cttagcgcac
     4981 gccttggtta taaaatggat gctgcctcag cggacgagat gttcctggag atcggcaggg
     5041 tctggccctc agtggctgga atgacttata gccgtttgga aaagcgtggt attcaatggc
     5101 cgtgtcccac cattgagcat ccgggaaccc cttacctttt caaaggaggg ttccccagag
     5161 gaaaaggaag atttacgccg gtgaagtaca ggccatctgc cgaactccct gatagcgaat
     5221 acccttttat cctctccacg gggagacagc tctttcagta ccataccggt acgatgacga
     5281 gaaagactac tgccattgag gttgtgtcac cgccttatgt ggagataaat cctgaagacg
     5341 caaagatgct ttccctggca aacggacaga aggtgaaggt gtcctctagg agaggaagta
     5401 ttgaaattac cgtgcgtgtt tctgaaagac cattgaaggg tgtggtgttc atcccgtttc
     5461 atttcaagga agctgctgcc aacgtcctca ccaataccgc attggatcct gtatgcaaga
     5521 ttcctgaact aaaggtctgc gcagtaaaaa tagaggctta acgaagggat acatcccgcg
     5581 acggacgtca gtttctcctt tccatacgaa aggtcttcag ccttctcctg gagatgattg
     5641 ttccaatagc agtaggtact ctgggccatt atttcttttt cctctgaacg gactgcctat
     5701 taatatatcg aagactgaga atttttacaa tatcaggcct agaaatttcc taatctaacg
     5761 ccctcatagc cataggggaa aagtccggct gacacacagg tttccggcgg cacgctgacc
     5821 acgcttgcaa tgggattcat ctttattcta aaatgcgttt gactctgaaa ttcaggggga
     5881 taaatggggt aacttttggc aaagacgatt cagaaatgac agccatctcg ttcctcttca
     5941 attcccttct gattgcgggg cactgtccca agagcaaagt cattaagtaa aacaattaat
     6001 cctttggata gcttgcacct ttgcttggcg gagaaaaata ataatgaacg gcaatataga
     6061 ttttcatatt ccagattgta accaatttcg taatggttac gaagcatata ccagagaaag
     6121 gcgaggggct gtttactttg aagatctttc gttaatttca gagaattggg gagaccccgt
     6181 tcttatggca caaggggttc aaagattaat tcgatcttgg catttttttt ttgcaaactt
     6241 tgatttcgac agtttggtta attgcattga taacaatttc caggatctca atgagtttag
     6301 agcgagaaac gtagtgagtt tagctgatgc tgatgacgtc aggattgaaa gtttgtttaa
     6361 ccatttccta gacgctctaa aacggacaag agacaatgca aaaagtcctg tatctgttgc
     6421 caaagcttta agtcctttag ctacgcattt ctttcctctt tgggattcaa atattgcatt
     6481 ttcgtacaat tgtttctacc ttgcagatat ctcagcgcct ccgtatatca gattctgcaa
     6541 aaaaatgaaa attatgtcag agcatgtaca acattgtgtt tcaaataagg atgatagatc
     6601 cctgttaaaa agaatcgatg aatataatta ttcaaaatat acaatgcatt ggatatgaga
     6661 aaaagtgcca agcaaaacag ttgcctgaca cagtgcaaac ggctaaagcc caaccatcga
     6721 tgcatcgttt gtgccgcaga cgttatctgc tcattgagga cagaacagga aaaagagtca
     6781 ataagatgaa agtctattct gtggacaaaa agacccaaga gaagatgtta tcatgggggc
     6841 gatacttatt ttgggctgag attctatgtc agcgcttcaa agattttata gattctcctc
     6901 atgatattga gaatagtgtt gatagctgga ctttctttgc attaatgtcg caatggtatg
     6961 ccagtttatg ggtagtaatt gaagggtggc agaaattggg ttttgatgat catgctataa
     7021 acgacttgat acagcaacgc tctgttttct gtgatttaat gaagcgatat aggaatggag
     7081 tgtttcacta tcaaccaaga atgatagagg agagattcct tgcttttttg agagagggcg
     7141 actaacatgc aatttgggtt aataatcttc acaatgaatt cttgagattt ttctgggaat
     7201 ggatcgataa ttttccggga accgcaatac agaaagagga atttcggcaa attatcataa
     7261 gcgttattgg atgggtacct gaaaatatcg tccctgcacg cattcaagaa cttgagcgga
     7321 tgacggaaga cgcagatgaa atgatagcac acgataaaaa atcggagaat ctctacacga
     7381 ggagtctaaa ggaagctatt accaatgcaa gaggtatctt aaaaaccatt caaaaggaaa
     7441 aggataagtc atcaaaaagg aatgtccgca acgccaatta acaaccaaga gaaaattacg
     7501 gacagatcct attatttctt ggcacatgga tgactgaaga gcaggaagaa agatgttggt
     7561 tgttctattt aacaaccaag gtagatgcta tctgaaagcc gccaccagaa tgaatatgat
     7621 attattattc aggaaaggac tttgatatga aacaaataaa acttatagta gaaaagcatc
     7681 cggacggata cgtcgcgtat cctttggggc ttaagggcgt tgttgtgggg caaggagaca
     7741 catatgaaga agcacttgca gacgtaagat ccgcaattca tttccatatc gaatccttcg
     7801 gggaggaagt atttgatgtt gatcccccta tccttgaggc tttcgtcgca gaggccgggg
     7861 tagaggttta atgcctaaat tcccactgtc atggtaaaat tatcacaagg aggagcaccg
     7921 tggccattcg atatatctta agtgaatacg tcgagcaagc tatggcacag gctgtctatg
     7981 ataaacttga agacggcacg tttgccggtc gtatttctgc ctgcaaagga gtgttggcct
     8041 ttgggcccac attgcgccag tgcgaggaag acctccgttc aactctcgag gattggattc
     8101 ttgtcggatt aaaacttgga caccctttgc cggtgatagg agaaattgat ctaaacaaag
     8161 agcattctcg tgagccagtg gactccgtgt aagcgccgcg attttatcag tcgtttacgg
     8221 aagctttgat ttgaaggccc tttctccgga accagacacc agttcatggt ctaccaacaa
     8281 caccgcctgt caatcccctc gaacactgaa tactccattc ctcagctgcg gatgatgatt
     8341 caggaagtgg aaagcataat tggtcgtata attgctgcgg atgaatggaa tcgtttataa
     8401 agagatggga attcttaaaa gacggcttcc ggcaattttg ggaatcatcg taggattcct
     8461 ctccataatg gccggctcaa aaatattatt gggaatctct tctcccaact atattgtgct
     8521 tcactggctg gttatttata acgtttcatt cggcgtcgtc tccgttcttg caggggcagg
     8581 actgtggcgc cttcggccat gggccatttc gctggcaggt ctcaccgctg cctctcacgg
     8641 ctttgtcctc gctctattaa ttgtgttctt tatatccggc aagcccgtgg cataccagag
     8701 cattctggca atgctttttc ggacaactgc ctggctggga atatttcttt ttgtgaggac
     8761 tcctaaaggg gacagttcaa tttgagactg cccgcaacgt tatcaagaat gtcctgcaaa
     8821 ggagttcttg aaggggatta tatgatattg tccaggttgc gtgagccgtg gatgactcta
     8881 tgaatttcaa ctgttctatt acgcactatg tagaacacaa gataagcctc gattatgagc
     8941 acacgataac cgaagtcttt tagtttttcg tctctcggta ttctcccaag gaaaggatga
     9001 ttttttaggt tgagaagctg cttgtcgagc ttttttatga atgcatcagc atttgcaggg
     9061 ctgtcattgg ctatccagtc aaagattgaa atgagatcgt caactgcgac cggaaggtag
     9121 cggagagcgt acttactttc catgcgttcg ttgacgcagt ttactcatga cctgggaatg
     9181 ggtatatcct ttggttcccg cagcagcctg ggcctgagca acagcaagtt tttcaaaaag
     9241 gtctatcctt gccttgagtc tttcataatg ctcaatgctc atcaccacaa ggtcgccctc
     9301 accgttcgta gtgagatata ccggttcatc ctgctcatgg cacagggaag cgatatcacg
     9361 tgttctgttt cgcaggcttg aaatagattt tattatcggc attgttactc ctcggcaatt
     9421 tgtttttata aatttatagt agaacttaat tatgataaaa gcaagtataa atttgtgtcg
     9481 cactgctgat taccgcacgt tgggctcctt tcagaaaaca gccgggtctt atttcgggta
     9541 ctattgatcg cttcttgttg gcataattgc ctgatatggt tttttctaaa accgatgtcc
     9601 ggtattcaga acaaacatca ggagcgaggc agataaacta aaggcgagaa cttactaaaa
     9661 tcccatcact gctgcccgtg ccatgctgat gacacctgca ggaagcacgc cgatgaggag
     9721 caccgccaga attgtcacgg tcagtgcaag gtttgtggca agggatgtgg agagcttgac
     9781 cacttgcctc ggttccttca tgtacatgtt catcacgatc ctgagataga agtaggcaga
     9841 tatggagctg aagatcaccg caacaatggc gagccatgta taccccgcat tcactgctgc
     9901 catgaagaga taaaacttgc ccacaaagcc tgcggtaggg ggtattccgg tgagggagaa
     9961 catgaagatg agcatcaatg ctgatgccag gggatgggac tttgccaggc cttcataatc
    10021 gctgatttct tcccccttaa atccctctgt cctcagcatg atcactattg caaatgcgcc
    10081 gatgttcata aaagcataga tgaagaggta attcatcatg ctcgccacac cctcagtccc
    10141 gccggcaata ataccgatga gggcgtagcc tgcgtgggcg atggatgaat aggcgagcat
    10201 tctttttatg ttggtctggg agagggccag tatgttcccc accgccatgg tgaggatcgc
    10261 tatggggatt attatgcctg cccagtctat ctggaggtca ccgaatgcca tgaagaagac
    10321 cctgcctaat gccgcgaatc cggcagcctt gggtcccacg gacataaagg cggtaatgga
    10381 tgtgggcgag ccttcgtaca cgtcaggtgc ccacatgtgg aacggcgcgg cagcgatctt
    10441 gaagccgaat gccaccacaa agagcatcat cgagagcatg gtcacggaat tatgccctat
    10501 gccgtttgca gcgatgtact tcgcgatggc gttcaggttc gtggtcccgg tcatgccgta
    10561 aatcaaggac attgcgtaga ggagaaatgc cgaggcaaag gcccccagga ggaaatactt
    10621 gagtgaggcc tcattggact tcgtgtcgtg cctgaaaaac cctgcgagga cgtacaggct
    10681 cagcgacatt aattcaaggc ctaggaacag cacaataata tcccctgcag aggccatgac
    10741 catcatgccc agggttgaaa aaagcatgag gctgtaatac tcaccgaaat tgaccttttc
    10801 cagtctgtaa ttctcaccga aattgagctt ttccagttct atgtatcgaa tggatatgag
    10861 tatggaaagc accaggctga tgagaaagat gagcttgaag aagaggctgt aactgtccgc
    10921 gatgtacatg cccgaaaaag tcacccctga ggaagccatc acaaaatacg caacaatacc
    10981 ggcggtgagt atgctcagga gcgcgattga cgcctttctc tttaccatga ggtccaggag
    11041 cagcactgcc atggccagaa cagccatgac aatctcaggc attgccggtc ctatatcagg
    11101 catccgcatc ataccctccg ctcagataca acaaattccc ggcagtattc aattcggttc
    11161 attttattat ctccattatc tttgcgactg cggtcatctc ctgtccgcct gagtggagcc
    11221 tctggaggag gtgttcaaca gacggatgca tgaaactcag gaatgtatct ggatagactc
    11281 ctatccagaa aataagaatc accatgggca ataacgtgat gatctccctc gtatccattt
    11341 cctggagccc aataagtttc tggttcgtct ccataaagaa tacccgctgg tacaaccaga
    11401 gcatataccc cgcgccgatg attattccca gggctgaaag cacacccgcc catttgctcg
    11461 cggtaaaacc gcccaggatg atgagaaact ccccgataaa tccgttcatt ccgggaagcc
    11521 ctatggatgc cagggtaaat accatgaaaa atgccgcgta gaggggaagc actgtggcaa
    11581 ggccgccgta atccctgatc tcccttgaat gggtcctgtc atacactact cccactgaga
    11641 ggaagagtcc tcctgtgaca atcccgtgat taagcatctg gaggatac
//
LOCUS       DATKOU010000100         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4363040, whole genome shotgun sequence.
ACCESSION   DATKOU010000100 DATKOU010000000
VERSION     DATKOU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4363040"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /gene="aroA"
                     /locus_tag="VMU21_04560"
     CDS             complement(<1..1279)
                     /gene="aroA"
                     /locus_tag="VMU21_04560"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HUO76831.1"
                     /translation="MDRIELKGGRCLRGELVPPPDKSISHRAVFFSSIAKGKSAVRNF
                     LRAGDTLSTVAAFRSLGIDIEDRGDELMVHGKGVHGLREPHTIIDCGNSGTTIRLLSG
                     IVSGNPFFSVLSGDDSLRTRPMQRVISPLREMGAEILGRDGDRYPPLAIKGKKLRAIR
                     YSLPVASAQVKSAILLAGLYSNGETEVTEPLRSRDHTERILPAFGARIKVEGLTARIY
                     GGDELYARDTTVPGDFSSAAFFIVAALLLKGSEITVRTVGINPTRTGLLTVLGRMGAE
                     VSMENIREVSGEPVADISCKGGMGLRAVDITREEMPLLIDEFPILCVAASQAEGLTSI
                     RGAEELRVKESDRIKAMTAELKKMGVEIEEYPDGVSIKGKTMLKGGKVESYGDHRIAM
                     SLAVAGLVAEGTTSIHNALCVNISFPIFFDEVKR"
     gene            complement(1266..2120)
                     /locus_tag="VMU21_04565"
     CDS             complement(1266..2120)
                     /locus_tag="VMU21_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HUO76832.1"
                     /translation="MFFPKVTILGVGLIGSSVALAMKKRRLCGHITGFGRREERLMRA
                     KELGIVDSFDLDPSRASLGSDFVLFSMPVGFFPGIAGMIRDSLKEGAVVSDAGSIKGN
                     IVHEMEKVLSNKTPFVGAHPIAGSEQSGIDAADADLFEKRRCVITPTDKTDRAALNTL
                     TELWRSLGAEVVIMTPEEHDRVFGAVSHLPHIIAYELMNSADELNHSYLAYAGQGFLD
                     TTRIAASSPEVWRDICMGNKENLIQFLDTFMGRLERVKGYLAGSDAASLEGEFSKAKT
                     LRDECGQD"
     gene            complement(2127..3152)
                     /gene="aroF"
                     /locus_tag="VMU21_04570"
     CDS             complement(2127..3152)
                     /gene="aroF"
                     /locus_tag="VMU21_04570"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HUO76833.1"
                     /translation="MDIIVLRPDATDEALRHLLKKLESKGLSAHVSKGTERTIVGVIG
                     DTSRVTEEEEDAIRVMTGVENVMRILKPYKLASRDFKTENTLIDVKGRVIGGKKIQVI
                     AGPCAIENKTLIMNIAEKVKEAGAAFIRGGAFKPRTSPYSFQGLGEEGLRYLADAREE
                     TGLPVVTELMDPRDIEAMVKYADIIQIGARNMQNFRLLLEVGSVRKPVLLKRGLSATV
                     KEWLMSAEYIMSRGNHQVILCERGIRTFETATRNTLDLSSVPLLKQLTHLPVVVDPSH
                     GVGKWDLVPPMAKAAVAAGADGLLIEVHTNPEEAMSDGEQSLKTEAFSVLMKELKAVA
                     SAVGREI"
     gene            complement(3234..>3466)
                     /locus_tag="VMU21_04575"
     CDS             complement(3234..>3466)
                     /locus_tag="VMU21_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUO76834.1"
                     /translation="EKGKQYLYKELRHLGVGYVPTEANFIYVPVEDSMGLYEKLLKKG
                     VIVRPMGPALRVTIGLAEENERFLDALKSVMR"
BASE COUNT          772 a         1049 c          762 g          883 t
ORIGIN      
        1 accgcttcac ctcatcaaaa aaaatgggaa acgatatgtt cacgcacaat gcattatgaa
       61 ttgatgtcgt cccttcagcc actaacccgg ccactgcaag ggacatggct atcctgtggt
      121 ctccgtaact ttccactttg ccgcccttaa gcattgtctt accctttatg gagactccgt
      181 cagggtattc ctcgatctcg actcccattt ttttcaattc ggctgtcatt gcttttattc
      241 tgtcagactc cttgaccctg agctcttcag ccccgcgaat ggaggtgagc ccctcggcct
      301 gtgacgcggc aacacagagt atgggaaact catcaatgag caggggcatt tcttcccgcg
      361 tgatgtccac tgcccgcaat cccatccccc ctttgcagga gatgtctgcc actggctctc
      421 ctgacacctc ccggatattc tccatgctga cctctgcacc catcctcccg agaacagtaa
      481 gaagacctgt tctcgtggga tttattccca ctgtccttac ggtaatttcg gaccctttca
      541 acaagagcgc ggcaacgatg aaaaatgctg ctgaagaaaa gtctcccggg actgtggtgt
      601 cccttgcgta gagttcatct ccgccataaa tccttgccgt caacccctcc actttaatgc
      661 gggctccgaa ggcgggcagt atcctctcgg tgtggtccct cgatcgcaaa ggctccgtaa
      721 cttcggtctc tccgttcgaa tacaggcctg caaggagtat ggctgacttg acctgagcgc
      781 tggcaacagg gagagagtac ctgatggctc gtaacttctt gcccttaatc gcaaggggag
      841 ggtacctgtc accgtccctt cccagaatct cggcacccat ttccctcaac ggggaaatca
      901 ccctctgcat cggccttgtt ctgagggaat catctcctga caagacagag aaaaagggat
      961 tgccggacac aatccctgag aggagcctga tggtggtccc tgaattgccg caatcgataa
     1021 tcgtatgcgg ttccctgagg ccatggacgc ctttcccatg gaccataagt tcatcacccc
     1081 tatcctcaat atcgattccc aatgacctga acgccgcgac cgtgctcaga gtatcccccg
     1141 ccctcagaaa attcctcact gcgctcttcc cttttgctat agacgaaaaa aataccgccc
     1201 tatgggatat ggacttgtca ggaggcggga caagttcccc cctgagacac cttccaccct
     1261 tcagctcaat cctgtccaca ttcatctctc aatgtcttgg ctttactgaa ttctccctca
     1321 agtgacgccg catctgaccc ggcgagatac cccttaaccc gttcaagcct ccccataaac
     1381 gtatccagga attgtatgag attctctttg tttcccatgc agatatccct ccaaacttca
     1441 ggagaactcg ccgcgattcg tgtggtatcc agaaagccct gccccgcata ggcaaggtat
     1501 gagtggttga gttcatcagc gctgttcatt aactcatatg cgatgatatg ggggagatgg
     1561 ctcactgcgc caaagaccct gtcatgctcc tccggagtca taattaccac ctctgcaccc
     1621 agggaacgcc ataactcggt aagggtattc agtgctgccc tgtcggtctt gtccgtgggc
     1681 gtgatcacgc agcggcgttt ctcaaagagg tctgcatctg cggcatcgat cccggactgt
     1741 tcgcttcctg caatgggatg tgcaccgaca aacggcgtct tatttgacaa tactttttcc
     1801 atttcatgca caatattccc ttttatgctt cccgcgtcgc tcactacggc cccctctttc
     1861 agtgagtccc ttatcattcc cgcaatgccg gggaaaaatc ccacgggcat ggaaaacaga
     1921 acaaaatcag acccgagaga agccctggac gggtcaaggt caaaggagtc aacaatgcca
     1981 agctcctttg ccctcatgag gcgctcctct ctcctgccaa acccggtgat atggccacag
     2041 agcctgcgct ttttcatggc aagggcaacg gaggaaccga tgaggcccac gccgaggata
     2101 gtgactttag gaaaaaacat gaaggcttat atctcccttc ccaccgctga agccactgcc
     2161 ttaagctcct tcattaatac actgaacgct tccgtcttga gagattgctc cccgtcagac
     2221 atcgcttctt ccggattggt atgaacctca atcaggagcc cgtcagcccc tgcggcaaca
     2281 gcggcctttg ccatgggagg cacgagatcc cattttccca caccgtggct tgggtccact
     2341 actaccggaa gatgggtgag ctgtttcaat aatggcacgg agctcagatc aagggtgttc
     2401 ctggttgcgg tctcaaaggt ccttattccc ctctcacaga gaatgacctg atggttaccc
     2461 cttgacatga tatactctgc tgacatgagc cactccttta ccgtggcgga cagtcccctt
     2521 ttcagcagga ccggttttct gacagagccg acctcaagga gcagcctgaa gttctgcatg
     2581 ttccttgcgc cgatctggat gatgtcggca tacttcacca tggcctcgat gtccctgggg
     2641 tccattaatt ctgtcaccac agggagccct gtctcctccc tcgcatcagc aaggtatcga
     2701 agcccctcct caccaagccc ctgaaaggaa taaggtgatg tccgcggttt aaacgcccct
     2761 ccccttataa acgcagcgcc cgcttccttc accttctctg caatattcat tatcagggtt
     2821 ttattctcaa tggcgcatgg cccagcaatg acctgtatct ttttgcctcc gattaccctt
     2881 cctttcacgt caatgagggt attctctgtt ttgaaatccc tgctcgccag cttgtagggc
     2941 ttgagtatcc tcatcacatt ttcgacaccc gtcatcaccc ttatggcatc ctcttcttcc
     3001 tcggtgactc tcgacgtatc cccgattacc cctacgatcg tcctttcagt gcccttggat
     3061 acatgggcac tcaggccttt ggactcgagc ttcttgagca gatgccgaag tgcctcatcc
     3121 gttgcatcag gtcttaatac gataatgtcc atttactcct ccattcatca aaaaattgtc
     3181 cagtgaagaa ccctgcggca agacagccac tcgctaaatg cctgaatggc cgatcacctc
     3241 atgactgatt tcagcgcatc aagaaacctc tcgttttcct cggctaaacc gatagtaacc
     3301 cgcagtgccg gtcccatggg tcttacgatc acaccctttt tgaggagctt ttcatagagt
     3361 cccatagagt cctcaacagg gacgtagata aaatttgcct cggtgggaac gtaccctaca
     3421 ccaaggtgcc ttagttcctt ataaagatat tgtttcccct tttcat
//
LOCUS       DATKOU010000101         4548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4365490, whole genome shotgun sequence.
ACCESSION   DATKOU010000101 DATKOU010000000
VERSION     DATKOU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4365490"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="VMU21_04580"
     CDS             <1..1947
                     /locus_tag="VMU21_04580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HUO76835.1"
                     /translation="KTRVIEYRVLYLMENKVNPHSLLLLTFTRSAAREMISRAAHHDP
                     RCKNVEGGTFHSFAYKQLKRYAKSMGLSESFSLLDEGDAAEAVYRCSVRLGFYEKEKR
                     FPKKDTLRALISASINKNISVGEVLKKEFPHFLEYSSDIENLRREYTSYKISKNYLDY
                     DDLLVYLRLLLENMEIRDRLSRKYRYIMVDEYQDTNTLQGDITYFLAENHRNIMIVGD
                     DAQSIYGFRGASHENIMEFPKKFPECTIIKLEENYRSTQSILDVANAVLENMKEKYSK
                     CLVSAKKQLGEKPHLLFFKDAHEESEWTAEKIKLFRDEGIPLGHQSVLFRSQYLSIPL
                     QAELSRRDIPFEVFGGLKFYETAHVKDIMSHIKILANPKDELAWNRVLMLLDGIGPRT
                     SEQISEEILGCASFDGIIQEVLQKYSRGHKYSEGLARLASLLKDVHSERMNVGEKFSI
                     VLEYYVPFLKERFDDWHLRLNDLETLRQVSMRYDFLEDLLEDFSVEPPERGVWRVQPS
                     TPDEEKPLTLSTIHSAKGLEWECVFLIGLMDGVLPVAFSLDHEDEIEEEQRLFYVGIT
                     RAKNHLFLSLHHEGYRGGITQFNKISRFVDVPNVLSKLSLEGVKKEKREAHSEIEEVL
                     SPYNKESLLQRVMDFLEKKGDIHS"
     gene            1944..2582
                     /locus_tag="VMU21_04585"
     CDS             1944..2582
                     /locus_tag="VMU21_04585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HUO76836.1"
                     /translation="MIHPTRTNLLLLKEKTLSVNNSVGILKARRQALIREFLATSVPF
                     LRSREDIRKTYGKAIGELKLSLGHEGRGTIESLSLVTKKDLAVEMAERNIWGLRYRDV
                     VMHESPVKTPDERNYDYQSTSPHLEESIFLFEKLVESMIEIATYESKLKRLGEEIVKS
                     TRRIRVLEERVLPELTHQIKVISQYIGERERETYYRLKRFKNIRTQPQKQPA"
     gene            complement(2592..3044)
                     /locus_tag="VMU21_04590"
     CDS             complement(2592..3044)
                     /locus_tag="VMU21_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HUO76837.1"
                     /translation="MKPTIPETGRVIRVDKDMAVILLHPGQSCKGCGAAEIGLCKSSG
                     AVSTLIARNKVNAVPGDTVRITLDKATQTRGLLLAYVIPVVCFISGVILGHIAGRALA
                     IPSLEVLTGFISLVGSAFYSLRKLKKLDSVSSMAIKEIVSDPVFLDCH"
     gene            3196..>4548
                     /locus_tag="VMU21_04595"
     CDS             3196..>4548
                     /locus_tag="VMU21_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HUO76838.1"
                     /translation="MDWHQREINEVIHELKSLPHGLSAEEARKRLEEYGPNELKEKKK
                     KTPLYMFLDQFRDFMILVLIAAALISGTIGEVSDTIAIVVIVVLNALVGFVQEYRAEK
                     ALAALKKMVDPTALALRDATPANVPASEIVPGDVVILEAGRVVPADLRLIETAQLKIE
                     EAALTGESVPVEKQVKALHEEALPLGDRKNMAYKGTTVSYGRGMGVVIATGMRTELGE
                     IAHMLQGEEEVRTPLQKRLLGFGRRISLVVLFICALVFGAGIFRGEPVILMFFTAVSL
                     AVAAIPEALPAVVTVALALGARKMVRQNALIRKLPAVETLGSVTFICSDKTGTLTLNR
                     MTVEEVYVDRELSKCRDWRPDMDNSPVSDPRSLLFTALALSNDAQKDSSGTPIGDPTE
                     IALYAAARDKGFEKEVLENKFARISEIPFDAERKCMTTFHKWSHGFISFTKGAIDSLV
                     E"
BASE COUNT         1266 a         1070 c         1136 g         1076 t
ORIGIN      
        1 aagaccagag taatcgaata ccgggtgttg taccttatgg agaacaaggt taatccccat
       61 tccctgctcc tcctcacctt taccaggagc gcagcgcgtg agatgatttc ccgggccgct
      121 caccatgacc cgaggtgtaa aaatgtggaa gggggaacgt tccattcctt tgcgtacaag
      181 caactcaaga gatacgcaaa gagtatgggg ctctcggaat ccttttccct tctggatgag
      241 ggggacgctg ctgaggcggt ctaccgctgc tccgtaaggc tcggattcta tgaaaaagag
      301 aagcgtttcc cgaaaaaaga caccctaagg gctcttatca gcgcatccat caacaagaac
      361 atatcagtgg gagaggtcct gaagaaagag tttccccatt ttctcgaata ttcgtccgat
      421 atcgagaatc tcaggaggga atatacctca tataagatca gcaagaacta cctcgactat
      481 gacgatctcc tggtgtatct cagactctta ctcgaaaaca tggagatacg ggaccgcctc
      541 tcgcgcaaat atagatatat tatggtggat gaataccagg ataccaatac actgcagggc
      601 gacattacct actttctcgc cgagaaccac agaaatatca tgattgtggg cgacgatgcc
      661 caaagcatct acggttttcg gggggcgtcc catgaaaata tcatggagtt cccaaagaag
      721 ttccctgaat gcacaatcat aaaactggag gaaaattacc ggagcaccca gtccattctc
      781 gatgttgcca atgcggtcct cgagaatatg aaagagaagt attctaaatg ccttgtatct
      841 gccaagaaac agctgggaga aaaaccgcac ctcctttttt tcaaagacgc ccatgaggag
      901 tcggaatgga ctgcagagaa aattaagctg tttagggacg agggtattcc cctcggacac
      961 cagtctgtgc ttttcaggtc ccaatatctt tccattcccc tccaagctga attgagcagg
     1021 agggatatac cctttgaggt attcggaggg ctaaagtttt acgaaaccgc ccatgtgaag
     1081 gacattatgt cccacattaa gattctcgca aatccaaaag acgaactggc ctggaaccgg
     1141 gtcttaatgc tccttgacgg catcggtcca agaacttccg aacagatctc agaagagatt
     1201 ctcggatgcg cctcttttga cggtatcatt caggaagttc ttcaaaagta cagcagggga
     1261 cacaaatatt ctgaaggttt ggcaagactc gcttccctcc tcaaagacgt ccatagtgag
     1321 cgaatgaatg tgggggagaa gttcagcata gttttggagt actatgtccc ttttcttaaa
     1381 gagagatttg atgattggca cctcaggctc aatgacctgg aaaccttgcg ccaggtatcc
     1441 atgagatacg atttcctgga ggatctcctg gaagattttt cagtggagcc gcctgagagg
     1501 ggcgtgtggc gcgtgcagcc atcaactcct gacgaggaaa aacccctcac cttatccacc
     1561 atccattctg caaagggatt ggaatgggag tgcgtgttcc ttatcggtct catggatgga
     1621 gtattgcctg tcgctttttc cctggaccat gaggacgaga tagaggaaga acagcgcctc
     1681 ttttatgtgg gaatcacccg ggcaaagaac cacctctttt tgtcccttca tcacgaaggt
     1741 taccggggag ggattaccca gttcaacaag atttccagat ttgtggatgt tccgaatgta
     1801 ctctcaaaat tgtcgctcga gggtgtcaaa aaagagaaga gggaggcgca cagcgaaata
     1861 gaggaggtcc tctcacccta taataaagaa tcgctgctcc agagggttat ggactttctg
     1921 gagaagaaag gggatattca ctcatgatcc atcccacaag gacaaacctg ctccttctta
     1981 aggagaagac actatctgta aacaacagcg tgggaatcct taaggcgaga aggcaggcat
     2041 taatcaggga attcctcgca accagcgttc cttttctgag gtccagggag gatatcagaa
     2101 agacttacgg caaggccata ggagaactta aactgagcct ggggcatgag gggaggggga
     2161 ctatagaatc tctatccctc gtcacaaaga aggacctggc tgttgagatg gcagagcgaa
     2221 acatctgggg cctgagatac cgggatgtcg tcatgcacga gtctccggtg aaaacacctg
     2281 atgagaggaa ctatgactat caatcgactt caccccatct tgaagagtcc atattccttt
     2341 tcgaaaagct tgtggaatca atgatagaga tcgcaaccta tgagagtaaa ttgaagaggc
     2401 tgggagaaga gattgtaaaa agcacccgaa ggataagggt ccttgaggag cgggtcctgc
     2461 ctgaactcac gcatcagata aaggtcatat ctcagtacat aggcgagagg gaaagggaga
     2521 cctactatag actgaaacgc tttaaaaata tccgcactca gccccagaaa cagccagcat
     2581 gaccctgcga atcagtgaca atcaagaaaa acggggtctg acacaatctc cttaatcgcc
     2641 attgaagaaa cgctgtccag tttcttcagt tttcttaagg aataaaatgc tgaacctaca
     2701 agcgagataa accccgtgag tacttcgagg gaagggatgg cgagtgctct ccctgcaata
     2761 tgtccaagga taacgcctga gataaagcag acaacgggga tgacataggc aaggagaaga
     2821 ccccttgtct gtgtggcttt gtccaaggta atcctcaccg tgtctcccgg aactgcattc
     2881 accttgtttc tcgcaatgag ggtggacact gcccctgatg atttacaaag ccctatttct
     2941 gctgctccgc aacccttaca cgattgtcct ggatgaagca atattacagc catatccttg
     3001 tccactctta tcaccctgcc ggtttccgga atcgtcggct tcataatgac attctatgaa
     3061 agcaatacat gcaacactat gttctaaatc ataaagtatg tcgctcagag cagatagtgg
     3121 cgtatactat cttctggtat tattattaag acagtggaat gctacccctc atttatgtca
     3181 aaaagggctt tgtgaatgga ctggcatcag agagaaatta acgaagttat ccatgagctg
     3241 aagtcacttc ctcacggtct ctccgcagaa gaggcccgta agagacttga agagtacggc
     3301 cccaatgaat taaaggaaaa aaagaaaaag acccctcttt atatgttcct cgaccagttc
     3361 agggacttca tgatcctcgt gctcatcgcc gcagcactca tatcaggaac catcggcgag
     3421 gtctcagaca ctattgctat cgtggtaata gtagtcctca atgccctcgt aggtttcgtc
     3481 caggaataca gggcggaaaa ggcattggct gcgcttaaga aaatggttga ccccactgcc
     3541 cttgcattga gagacgctac gcctgccaat gttcctgcct ctgagattgt tccgggcgat
     3601 gtggtgatcc ttgaggccgg aagggtcgtg cccgctgatt taaggcttat cgagactgcc
     3661 caactgaaga tcgaggaggc cgcacttacc ggagaatctg tacccgtgga aaaacaagta
     3721 aaagcccttc atgaagaagc attgcctctg ggtgatagga agaatatggc gtacaaaggc
     3781 acgaccgtct cctatggcag gggcatgggc gttgttatcg caacagggat gaggactgaa
     3841 cttggcgaga tcgcccatat gctccaggga gaggaagagg tgaggacgcc tcttcaaaaa
     3901 agactcctgg gctttggcag gagaatttcc cttgttgtgc tcttcatatg tgctcttgtt
     3961 tttggcgcgg gaatcttcag gggcgagcct gtcatcctca tgttctttac tgccgtctcc
     4021 cttgcagttg ccgcaatacc cgaggccctt cctgcggtgg tgacagtagc tcttgccctg
     4081 ggggcacgga aaatggtaag acagaatgcc ctcataagaa agcttcctgc tgtggaaacc
     4141 ctcggctcag tgacctttat ttgctctgac aagacaggca cgctcacgtt gaacaggatg
     4201 accgtcgaag aggtatatgt ggacagagag ttgtcaaaat gtagagattg gagaccggat
     4261 atggacaatt ccccggtatc tgatccccga tccttgctct ttaccgcact tgcgctgagc
     4321 aacgatgcgc aaaaagactc ttcaggaact cccattggcg atccaaccga gatagcactc
     4381 tatgccgcag ccagggataa gggatttgaa aaagaggtgc tggaaaacaa attcgcaagg
     4441 atctcggaaa taccctttga tgctgagaga aaatgcatga ctacatttca caaatggagt
     4501 cacggcttta tttcatttac caagggtgcg attgattctc tcgttgag
//
LOCUS       DATKOU010000102        13446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3211664, whole genome shotgun sequence.
ACCESSION   DATKOU010000102 DATKOU010000000
VERSION     DATKOU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 13446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13446
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3211664"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VMU21_04600"
     rRNA            complement(<1..249)
                     /locus_tag="VMU21_04600"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            742..2529
                     /gene="uvrC"
                     /locus_tag="VMU21_04605"
     CDS             742..2529
                     /gene="uvrC"
                     /locus_tag="VMU21_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HUO76839.1"
                     /translation="MLKKKISNVPQNPGVYLLKDQKERVIYVGKAKNLKNRLKSYFQR
                     PGDLDPRKLSMVKLVADFSYIITKNELEALILEATLIKQHKPKFNVVLRDDKNYPYLK
                     LTIKEDWPKLEVVRRVMKNGSLYFGPYVPAQGMWDILAFTRRNFPIRICNYSLEKPMR
                     PCIQYQMGRCPAPCAGKVSRNEYMKIVDEVKLFLKGQRGELLDKLQKKMITLSQELKF
                     EEAARIRDRIKNITRAWESQRVVAPELGDMDVIGYYSDGVDVVFNVFFIRDGILTGTK
                     DFHVRDAGNLPTGEVLGTFIGLFYAKEVIPPGEIIVGNRPNEIRNLKTWLRKKRGKQV
                     KITVPKEGKRQELLRMADENAKQLYKSGKSTVPDETLEALRDRLKFPSSPHSVGAFDV
                     STIAGSESVGAFIYWSEGEFKKNLYRHLRIKGVSGIDDCSMMNETITRTLKNLKDRIP
                     DLIVIDGGKGQLEIAKEVINTNEIMRNDGRPPMLIAIAKDPDRAFTTSSEIMNLEDRS
                     PESLLLKKIRDEAHRFAVSYHRKLRGSKFVESPLEKIHGIGRKRRLELLRRFGSIDDI
                     RNAPLEEISKLKGFNKMVAQNLLSELRRQ"
     gene            2526..3083
                     /locus_tag="VMU21_04610"
     CDS             2526..3083
                     /locus_tag="VMU21_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76840.1"
                     /translation="MMLPILLWVLILLFPICSDAAYKIYLKNGSVISGVSSYEKSNGD
                     VDIFFGGGSMGIPAKDILKIEETESREMDFRMQQAPEGREVAPPPPPPPTQTIDRSPQ
                     VDSLKAQIEGVDAELKATETEEADLVQRINDRKNVRLTYNSLQLRQLESDLAPLRQQL
                     SDVQMKKGALLQKKGDLEAQLKALQ"
     gene            3110..4042
                     /locus_tag="VMU21_04615"
     CDS             3110..4042
                     /locus_tag="VMU21_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HUO76841.1"
                     /translation="MTIYISGSLAYDRIMDFPGKFSDHILPDKIHILNVCFTVNGMVE
                     KFGGTAGNIAYSLSLLHEKPIILATIGKDYQGYFDWLKNNALPSEGIKIISEEFTAGA
                     YITTDKADNQITGFNPGAMKYPSEYAFKDPDPGDSLSLIAPGNLKDMVEYSSSYRSRG
                     IPYICDPGQSLTAWDSKDLAEWIHGSMILISNDYELEMIMNMTGMDKKGLLGLTKTII
                     TTLGEKGSLVSARISDHRIPPAETDRVVDPTGAGDAYRAGLIKGLVTGKDIVTAAKMG
                     SVAAVYAIEAYGTQEHRYSFEDFVERYTNNFGEM"
     gene            4090..5133
                     /locus_tag="VMU21_04620"
     CDS             4090..5133
                     /locus_tag="VMU21_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76842.1"
                     /translation="MINEQAIRTYLLKQFRDVLHVDISKLGSGVQGSGFLAELKTDKG
                     ATSYVVKSLISEGFGHDYPSDRAAVFLLDLDEFSNMPRHVKAIDVLAEMEDGSVRSIG
                     GGKEYYLLMERGEGRDYFHDLNEYSQKEKLDDSDVKKIRAMTSYLASIHNVKKDSQTL
                     YWRKLRDSVGHGECLMGVLDMYPEGILPYTEMSEIIKKSVDWNYRLKPKFSRLSQIHG
                     DFHPGNIWFKNETDFILLDRSRGPWGEPADDVTALTVNYIFFSIKHHGTVKGAYLEGL
                     RLFFEDYLRQSGDNEIYSIVQLFYAFRGVVVANPVFYPELTDEKRTVIFRFIHNVLDS
                     EKFEPERINEFLR"
     gene            complement(5420..6358)
                     /locus_tag="VMU21_04625"
     CDS             complement(5420..6358)
                     /locus_tag="VMU21_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO76843.1"
                     /translation="MNRNKPLISILTLVYNHSAFIELTIDSVLNQTFHNWEWIILDDG
                     STDSTGEMIKKIKDSRIHYSLQEHMGFDHLTGTYNSALAMCGGDFIALLDGDDYWPQY
                     KLEIQMKSFDDPNTILSYGECFVINQKGNKIDHIRLPEDLSIASNNPIGSALRKLLVD
                     EWCFMVPPTVMLRKSVLLGIGGFKEAKGLGEDFPTWLMLSLEGGFSAIPRCLGYYRKH
                     PFSVTATQNPIVYLGNQISFFRDFILKNNQKLENIGFSVDMDKLEKRWAKLSIKIKII
                     YFIRILSSPLRIDLVNLIVSAKSKLRKLMNALLKRS"
     gene            complement(6399..7508)
                     /locus_tag="VMU21_04630"
     CDS             complement(6399..7508)
                     /locus_tag="VMU21_04630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HUO76844.1"
                     /translation="MIEWENLAKTNKPFFDEYKARFSEILESGWFILGESVTEFEREF
                     ARYIGSKYCVGVGSGFDALVLSLKAFAFKPGSEVIVPANTYIATILAVLQNNLQPVLV
                     EPDSETCNIDPDKIEEKITSKTVAIIVVHLYGKACDMDRIMRFAKDFDLKTIEDCAQS
                     HGAKYKGKTTGSFGDVAAFSFYPTKNLGALGDGGAVVTDDPDFAEKIRVFRNYGSQRK
                     HLYNLIGFNSRLDELQAGFLLIKLKNLDRINRRKRVLARLYLDNLNSEFIKPVIQDGY
                     FDVYHIFAIRHPRRDELKEYLKNREITTEIHYPVPPHKQTALHGVLPEQAFPVSEEIH
                     NTILSLPCAFFHTEEDVMRVIEALNEFLQERHYSK"
     gene            complement(7505..7870)
                     /locus_tag="VMU21_04635"
     CDS             complement(7505..7870)
                     /locus_tag="VMU21_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HUO76845.1"
                     /translation="MAYTIELRTITDDRGSLTVIEKLLPFEIKRVFYVYNVPGEGLRA
                     GHRHRKELSALVSLNGTCVVKVLGFSEQHFVLDRPDLCLVLLPGDWHEIMDFSEGAIL
                     LVLSSEYYDPTDYVYEKLE"
     gene            8306..9442
                     /locus_tag="VMU21_04640"
     CDS             8306..9442
                     /locus_tag="VMU21_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76846.1"
                     /translation="MKRFALDIDVVGACNLRCPSCPQGNIKNYSLPHGFMEPDLLALI
                     IEKAKSECRVTGVALFSWAEPMLHPKLPDLIRIVQDAGISCYLSSNLNVQRNADAIMA
                     ANPFSFRISASGFTQEVYSYSHRGGDIEKVKKHMVELAEAKKRNNATTRIHVFYHRYK
                     HNLREEPLMREFAASLGIDLQPGWALMLPVEKVLGYMDDDASDFPLTGEDRQLIDRLA
                     LPLKEALNISQKYKNQTCSLREMHISMDFQGNVQLCYGIFHARRFTLGNYLTMPLDEI
                     QRLRQSHNMCSRCIRRGAHVYMTYGADELGEAAMANIAPEDAELLDLRYELAQQRLRR
                     RLAKAYQKVLAGAITPEQRTVLGVQFDRMQRFVGLTKRSLFRKV"
     gene            complement(9467..10312)
                     /locus_tag="VMU21_04645"
     CDS             complement(9467..10312)
                     /locus_tag="VMU21_04645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUO76847.1"
                     /translation="MSDKNSILIVDDDETLLPMLKEGFELQGYRCEAARSGEAALELV
                     KNISFDILVADIVLPGLKGFELIEKAKRLRPDTVAIIMTGFINKYTYDTAIEAGASDL
                     IKKPFTLKELMIRIEHAQVLKHLRETSVIDELTGLYNRRGFFTVAEQQVALAKRERRG
                     MVMLYAGLDDLEIIHDRWGSQEGDRALIASAVILKATFRKSDIISRIGEDAFVILTIG
                     VAGDDIDKITGRLQKNIDAHNAMNGRDYTLSMSLCKLHYDPQNSRPIDELLAYGEDMM
                     RKKKP"
     gene            complement(10500..10853)
                     /gene="atpE"
                     /locus_tag="VMU21_04650"
     CDS             complement(10500..10853)
                     /gene="atpE"
                     /locus_tag="VMU21_04650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HUO76848.1"
                     /translation="MKRIVAVMTLALALLWVCAPLALAEEAAAPASASTGKIYYYALA
                     ALGCGLAIGLAALGTGIGQGIGLSKACEGVARNPGVSGKITTTLIIGLAMIESLAIYA
                     LVVVLIILFMNPFKL"
     gene            complement(10885..11556)
                     /gene="atpB"
                     /locus_tag="VMU21_04655"
     CDS             complement(10885..11556)
                     /gene="atpB"
                     /locus_tag="VMU21_04655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HUO76849.1"
                     /translation="MHEESLLMDLIINPHVVPHYVSYAFLASFLLVGVALVMRGSLAL
                     VPKGVQNFMEVMVESLYKLAEENIGHHWGKYFFPLIGTLGLYILVSNFMGLIPGFTAP
                     TSNINMTASMAVPVFLATHYYGIKVHGFKYIKHFLGPIMWLAPMMFIIEFIGHLVRPV
                     TLSVRLFGNMIAKHMLLFALGLLVPLIVPVAILGLGVLVSLIQAFVFVLLTSLYLAGS
                     VEEAH"
     gene            complement(11674..12567)
                     /gene="prmC"
                     /locus_tag="VMU21_04660"
     CDS             complement(11674..12567)
                     /gene="prmC"
                     /locus_tag="VMU21_04660"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HUO76850.1"
                     /translation="MIRASEGMREVSKVLGGYGIEDPRKEAEIILTQCLDLERVALYR
                     DNPILSPLQVETLRKVLERRGLREPIQYITGHVDFYGLTIKVGPGVLIPRPETELVVE
                     EVIKTVKSRQSEVINQRPKDPGQSTTKRRLKVLDLCTGSGCLALAMAKEFPGAEVIGT
                     DISGRALKYARQNALTNGIENVTFLKGSLFEPLRGMRFDIVVSNPPYIRRTEIETLQP
                     EIKDWEPMEALDGGEDGLKFYREILLSSGEHLEGDGFMVMEVGDGEAGDVAAIAKSLG
                     FRNISVHKDYFAVERVLRIAF"
     gene            complement(12564..>13446)
                     /gene="prfA"
                     /locus_tag="VMU21_04665"
     CDS             complement(12564..>13446)
                     /gene="prfA"
                     /locus_tag="VMU21_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HUO76851.1"
                     /translation="ELLKGGDGDMKELAQAELDELRVKRPVIEEELKIMLLPKDPRDD
                     KSVILEIRAGTGGEEAALFASNLFRMYSKYAESRRWKVDVIDSSPTGLGGLKEIVAAI
                     EGKGAYSRLKYESGVHRVQRVPVTEASGRIHTSAATVAVLPEAEDVDIKVEEKDLRID
                     TFCASGPGGQGVNTTYSAVRIVHIPTGMIVQCQDERSQIKNKDKALKVLRSRLYDLEM
                     ERQEKERALERKTQVGTGDRSERIRTYNFPQNRVTDHRIGLSLHKLEQVLEGNIDEVI
                     DALITHYQTERLREALK"
BASE COUNT         3677 a         3206 c         3040 g         3523 t
ORIGIN      
        1 ccgcctttcg gcgggttgcc cccctcttaa gggtaggtta cctacgtgtt actcacccgt
       61 ccgccactaa actagttcat agcacattca gctcatagcc cttagctgat tgctcatagc
      121 aaattacctc tcccttgcca tgagccatga accatgagct aattgtgcta tgaactagtc
      181 ccgtccgact tgcatgtgtt aggcacgccg ccagcgttcg ttctgagcca ggatcaaact
      241 ctcatttgat actgggtaaa actctcaaat taacgtggcc tactttttta gttttcaaag
      301 aacaccccgc aatacccggg gttcttgagc cctctgaggc tttcgctaaa tgggtatctg
      361 gcaggtgctt aggcttcccc tctacaaaat catgcattgg ggcgtgcatc accacattct
      421 gcacgtagac tcccttcttt tatgctcttc cctcagctca cttagccccg agatgctgca
      481 gagaaaaaaa gaaccttcac aaatgagcct ttttactttg agcccattta atgtattccc
      541 tttgttgcct ctatatttag aataacattt cttgggatat agtcaataga aaactattgg
      601 caattatgag acaatatctg gttcaaaatc ataatattca attctttcta ttattttctc
      661 taattatcac gatatttagt gatatatctc acattattta taaccttgac gcacataatg
      721 tgctctgtta tcatttcagt tatgcttaag aaaaaaatat cgaatgttcc acaaaacccc
      781 ggagtttatc tgcttaagga ccaaaaagaa agagtgattt atgtcgggaa ggccaaaaat
      841 ctcaagaatc gcctaaaatc atacttccag agacctggag atcttgatcc gaggaaactg
      901 tccatggtaa agctcgtcgc agacttcagc tatattataa ccaaaaacga actcgaggcg
      961 ctcattcttg aagcgaccct catcaaacag cacaaaccca agtttaatgt agttctcaga
     1021 gatgataaaa attatcccta cctgaagctt acaataaaag aagattggcc taagctggaa
     1081 gtggtgagga gggttatgaa gaacggttcc ctctattttg gaccttatgt cccggcccag
     1141 ggcatgtggg atatcctcgc ctttacaagg aggaacttcc ccataaggat ttgcaattac
     1201 agcctggaaa agcccatgag accttgtatt cagtaccaga tgggcagatg tcctgctcca
     1261 tgtgcgggca aagtaagcag gaatgagtat atgaagatag tagatgaggt aaaactcttt
     1321 ctaaaagggc aaagggggga actcctcgat aaactccaaa agaagatgat cacactctct
     1381 caggagctta agtttgagga agctgccaga ataagggaca ggatcaagaa cataacacgt
     1441 gcatgggaat cccagagagt tgtggcaccg gagcttggag atatggatgt aataggctat
     1501 tattctgatg gcgtggacgt agtgttcaat gtctttttca tcagggatgg aatactcact
     1561 gggacgaaag acttccatgt aagggacgcg ggcaacttac caacgggaga agtcctcggt
     1621 acttttatag ggctttttta tgcaaaggag gtaatacctc ccggagagat cattgtaggg
     1681 aataggccca atgaaataag gaatctcaag acatggttaa ggaaaaaaag agggaagcaa
     1741 gtaaaaataa cggtgcccaa ggagggcaaa agacaggaac tcttgcgcat ggcggatgag
     1801 aatgccaaac aactctataa gagcggaaag agtacagtac cggatgaaac gttggaagcg
     1861 cttcgtgatc gtctcaaatt cccctcatcg ccccacagtg tcggtgcatt cgatgtctca
     1921 accatcgctg gaagtgaatc tgtaggtgcc ttcatttact ggtcagaagg agaattcaag
     1981 aagaaccttt acagacatct caggattaag ggcgtgtcag gaatagacga ttgctcaatg
     2041 atgaatgaga caattacaag aacgctgaag aaccttaaag acaggatacc ggacctgatc
     2101 gtaattgatg gagggaaagg acagctcgaa attgcaaaag aggtaatcaa tacaaacgaa
     2161 ataatgcgga atgacggcag accgcccatg ctcatcgcca ttgctaagga tcctgaccgg
     2221 gctttcacta cttcatctga aatcatgaat ctcgaagata ggagcccaga gtctctactc
     2281 cttaagaaga tccgtgacga ggcacatcgt ttcgcggtga gttaccacag aaagcttcgc
     2341 ggcagtaagt tcgtggaatc acctctggaa aaaatccacg gaataggaag gaagcggaga
     2401 cttgaacttt tgcgccgttt tggtagtata gatgatatca ggaatgcccc tctggaagag
     2461 atctcaaagc tgaaggggtt taataaaatg gttgctcaga atctattgag tgaattaagg
     2521 aggcaatgat gttaccaatc cttttatggg ttttgatcct gttatttcct atctgttccg
     2581 atgcagcgta taaaatctat ctcaagaatg gctcagtaat ctccggggtg agctcatatg
     2641 aaaaaagcaa tggggatgtg gacatctttt ttggaggggg ttcgatgggg attcccgcaa
     2701 aagatatcct taagatagaa gagaccgaaa gtcgcgaaat ggatttccgc atgcaacaag
     2761 cacctgaagg aagggaagta gctcctccgc ccccgcctcc cccaacgcaa actattgaca
     2821 gaagcccgca agtggattcc ctgaaagcac agattgaggg tgttgatgca gagttaaaag
     2881 ccacagagac tgaggaagcc gatctggttc aacggatcaa tgatcgaaag aacgtccggt
     2941 tgacttataa ctctctccag ttgagacaat tagagagtga cctagctcca ctgaggcagc
     3001 agctgtcgga tgttcagatg aagaaggggg ctcttctcca gaagaagggt gacctggaag
     3061 cccagctcaa agcactccaa tagccggatt gctggaaaca aattctccta tgaccattta
     3121 tatatcgggt tccttggcat atgacaggat catggacttt ccagggaagt tttctgatca
     3181 cattcttccc gacaaaatcc acatccttaa cgtatgtttc accgtaaacg gcatggtgga
     3241 aaaattcgga ggcactgcag gcaacatcgc atactcgctc agcctcctgc atgaaaagcc
     3301 cattatcctt gcgaccatag gcaaggacta tcaaggatac tttgactggc tgaaaaacaa
     3361 tgctctcccc tcggagggaa taaaaattat cagcgaagaa ttcactgccg gggcgtatat
     3421 caccaccgat aaagccgaca atcagattac tggattcaac cctggagcca tgaaatatcc
     3481 ctcagaatac gcattcaagg accccgatcc cggagactct ctctccctca tcgcaccggg
     3541 taaccttaag gacatggtcg aatattcgag cagttacagg agcagaggga taccatacat
     3601 ttgtgaccca ggacagtcac tcacggcatg ggacagtaaa gacctcgcag aatggattca
     3661 tggttcaatg atattgatct cgaatgacta cgagctcgag atgatcatga acatgaccgg
     3721 gatggacaag aagggattac ttggattaac aaaaaccatc atcaccaccc tcggagaaaa
     3781 gggttctctc gtaagcgccc gcatctccga ccaccgtatc cccccagcgg aaacggatag
     3841 agtagtggat cccacagggg caggtgatgc ataccgtgca gggctcataa aagggctggt
     3901 tactggtaag gatatcgtga ctgcggcaaa gatgggctct gtcgcagcgg tatatgccat
     3961 tgaggcatac ggaacccagg aacatcgcta ttcgtttgaa gacttcgtgg aaagatacac
     4021 aaataatttc ggagaaatgt aagagctgac aagcctgcac acttgattgc ttcgcgccgg
     4081 catgcattga tgataaatga acaagccata aggacctacc ttctgaaaca attcagggat
     4141 gtccttcatg ttgacattag caagcttgga tcaggggtgc agggttctgg ctttctcgca
     4201 gagttgaaga ctgataaggg tgctacttcc tatgtggtaa aatccctcat atccgagggc
     4261 tttggccatg attatccttc tgacagggct gccgtatttc tcctggacct tgatgaattc
     4321 agcaacatgc caaggcatgt caaggcgata gatgtgcttg cagagatgga agacggttcg
     4381 gtcaggtcta tcggaggggg aaaggaatat tacctgctga tggaaagggg agaaggcagg
     4441 gattatttcc atgatttgaa tgaatattct caaaaagaga agctcgacga ttctgatgtc
     4501 aaaaagataa gggccatgac gtcatactta gcgagcatcc ataatgtcaa gaaggattcg
     4561 cagacccttt actggagaaa actgagagat tctgtgggac atggagagtg cctcatgggt
     4621 gtccttgata tgtatcctga gggaatcctt ccctacacag agatgtcgga aatcatcaag
     4681 aaatcggttg actggaatta caggctaaag cccaaattca gcagactttc acagatacac
     4741 ggcgattttc accccggcaa tatctggttc aaaaacgaaa cagattttat cctcctcgac
     4801 aggagtcgcg ggccatgggg agagccggct gacgatgtga ccgcattgac cgtaaactat
     4861 attttcttct caataaaaca tcatggaaca gtaaaaggtg cttaccttga agggcttagg
     4921 ctattcttcg aagattattt gagacagtca ggtgacaacg agatatattc tatcgtgcaa
     4981 ctcttctacg ccttcagagg tgttgtggtg gcaaaccctg tcttttatcc tgaactcacc
     5041 gacgaaaaga gaacggtgat attcaggttc attcacaatg ttctcgattc agagaagttt
     5101 gagccggaaa ggataaatga gtttctgagg taattggagg tagcaatgtg taaaaagttg
     5161 ctagaaataa ctctttcact ttcttggtcc tgatggaaag gatctgtatt gttcaacgga
     5221 gcactcaaaa tctattttcc ccacgctata aaacaaatga cgatgctatt ttaatttatt
     5281 ctccaaatga tggacctaaa aggaaaatcc ttaaagtttt agaaggtcct tcttcaatgg
     5341 gtttaggtat cggaataagg tgcagtcagc atttatatta acgatctgac acgcacacaa
     5401 actaaagagg cagcttgcgt caagaccttt ttaacaaagc attcattaac ttgcgcaatt
     5461 tcgatttggc agatactatc aaattgacca agtcgatcct taagggcgag gaaaggattc
     5521 tgatgaaata aatgatttta atctttatag ataacttagc ccagcgtttc tcaagtttat
     5581 ccatatcaac ggagaaaccg atgttttcca gtttctggtt attcttgagt atgaagtctc
     5641 tgaaaaagct aatctgatta cccaaataaa cgatcggatt ttgagtggca gtgacagaga
     5701 acgggtgttt tcgatagtat ccaagacatc tgggaatggc tgaaaatccc ccttccaagg
     5761 aaagcatcag ccacgtgggg aaatcttccc cgagtccctt ggcttctttg aaacctccaa
     5821 tgccgagcaa tacactcttc cgaagcatga ccgttggagg aaccataaaa caccattcat
     5881 caaccaataa cttccttaga gcagagccta tcggattatt actggctata ctgagatctt
     5941 ccgggagtcg gatatgatct atcttatttc ctttttgatt tattacaaaa cattcgccat
     6001 aacttaggat agtattaggg tcatcgaagc ttttcatctg aatctcaagc ttgtactgtg
     6061 gccagtaatc atcgccatcc agcaatgcaa tgaaatcacc gccacacatc gcgagtgcgc
     6121 tattgtaagt acctgtgagg tgatcaaacc ccatgtgctc ctgaagggag tagtgtatcc
     6181 tgctgtcctt aattttcttt atcatttcac cagtgctatc agtagaacca tcgtcgagga
     6241 tgatccattc ccagttatga aatgtctgat ttaagacact atcgatagtc agctctataa
     6301 aggcactatg gttgtaaact agggtcaaaa tacttataag tggcttattc ctattcatat
     6361 ttagctacaa gataaccgcc gtttatctaa ttcattattt atttgctata gtgtctttct
     6421 tgcagaaatt cattcaaagc ttcaattacc ctcattacgt cttcctcagt atgaaagaac
     6481 gcacacggaa gagataaaat tgtattgtga atctcctctg agacagggaa agcttgttct
     6541 ggtaatacac catgcaatgc tgtttgtttg tggggtggaa cgggataatg tatttcggtc
     6601 gtaatttcac ggtttttcaa gtactctttc aattcatccc ttctcggatg tcgaatagca
     6661 aagatatggt acacgtcaaa atagccgtct tgtattaccg gtttgatgaa ctcactattt
     6721 aaattatcca gatataatcg tgccaatact cgtttacgtc tgttaatccg gtccagattc
     6781 tttaatttga tgaggagaaa gcctgcttgt agttcgtcca atcttgagtt aaatcctatc
     6841 agattgtaaa ggtgttttct ttgtgatccg taatttctga acaccctgat tttttctgcg
     6901 aagtccggat catcagtaac aacagcacct ccatcaccca aagcgcctaa gttttttgta
     6961 ggataaaaac taaatgcggc aacatctcca aaagaacccg ttgtctttcc tttgtatttg
     7021 gcaccatgtg actgcgcaca atcttcaatg gttttcaggt caaaatcttt ggcgaatcgc
     7081 ataattctat ccatgtcgca cgctttaccg tacaaatgca cgacaatgat cgcaacagtc
     7141 tttgaggtta ttttttcttc gattttatcc gggtcgatat tgcaggtctc actatcaggc
     7201 tcgacgagaa cgggctgtag gttgttttga aggaccgcaa gaatagtggc tatgtacgta
     7261 ttggccggaa cgattacttc actcccgggt ttgaaggcga aggctttcag agagaggacc
     7321 agtgcatcga atccagaacc tacacctaca caatatttac tcccgatata acgtgcaaat
     7381 tctctttcaa attcagtgac actctcgccc agaataaacc agccgctctc aagtatttcc
     7441 gagaatctgg ccttatattc atcaaaaaag ggcttattcg ttttagcgag attctcccat
     7501 tcaatcattc aagcttctcg tagacataat ctgtgggatc atagtattct gaagaaagta
     7561 ccagcaagat ggcgccctct gaaaaatcca ttatttcatg ccagtctccc ggcaaaagaa
     7621 ctaaacataa gtctggtctg tcaagaacaa agtgttgttc tgagaagcca agcaccttca
     7681 cgacgcacgt tccattcagg gagactaagg cggatagctc ttttcggtgt ctatgtcctg
     7741 cacgtaaccc ctcaccgggt acgttgtata cgtaaaaaac tctcttgatc tcaaagggga
     7801 gtaacttttc aatgacggtt aaactccctc ggtcatctgt tattgtccgt aattcaattg
     7861 tgtatgccat ttcaatcagt ccaggacaaa atcatagata cccacgggca tcgtggtact
     7921 tcaaaggagt ataagcttcg cttgaccgac tccgcctgac atcccctgca tcgctcaggg
     7981 caacattcgg tccacctttt ggctcaaata tagactctcg aaaggaggca agtgcgtata
     8041 tggtctttgt gtattgccac gtcttgtttc atgatagagt gcgccgttgt attctatcct
     8101 taacgatcct gccgttgtgt tattttatta gataacccat ttttatgcaa tattatcgtc
     8161 ctgaccctca actgccattt catctgcttg aataatcctg gagcccgaga actgccccac
     8221 aaaatcaaga agatgttgca gatatgtcta cattttctat agaataaaaa atcacagccc
     8281 cgatgaacac tttcttatca tctccatgaa gaggtttgca cttgacattg atgtagtggg
     8341 cgcctgtaac ctgcggtgcc cgtcctgtcc tcagggcaac ataaagaatt atagtctgcc
     8401 tcacggcttt atggagcctg acctgcttgc ccttattatc gagaaagcca agtctgagtg
     8461 ccgtgtgact ggcgtcgctc tgttcagctg ggccgagcct atgctccatc ccaagctccc
     8521 tgatctgatt cgtatcgtac aggatgcagg catcagctgt tacctgagtt caaacctgaa
     8581 cgtacagagg aacgcggatg ccattatggc agctaatcct ttcagcttcc ggatttcggc
     8641 ctctggtttc acccaggagg tttacagcta ctcccacagg ggtggggaca ttgaaaaggt
     8701 gaagaagcac atggtggagc tggcggaggc gaagaaacgc aacaacgcta ccacacgcat
     8761 ccacgtattc taccaccggt acaagcacaa tctgagagag gagccattga tgcgagagtt
     8821 cgcggccagc ctcggtatcg atttgcagcc gggatgggcc ctcatgcttc cggttgaaaa
     8881 ggttcttggt tatatggatg atgatgcctc cgacttccca ttgaccggag aggaccgtca
     8941 actgattgac cgcctggcac ttccgttgaa ggaagctctt aacatctcac agaagtacaa
     9001 gaatcagacg tgcagtctgc gggagatgca tatcagcatg gactttcagg gcaacgtaca
     9061 gctgtgctac gggatctttc acgcccggag attcaccctg gggaattacc tgaccatgcc
     9121 actggacgag atccagaggc ttaggcagtc ccacaacatg tgcagccgct gtatacgacg
     9181 cggcgcccac gtgtatatga cctatggtgc agacgagtta ggggaggcgg ccatggcgaa
     9241 tattgcgcca gaggatgccg agcttttgga tttgcgttac gagcttgcac aacagcggct
     9301 gcgccggcgc ttggcaaagg cgtatcaaaa ggttcttgcc ggagccatca cccccgaaca
     9361 aaggacagta cttggagttc aatttgaccg aatgcagcgt tttgtcggcc tgacaaagcg
     9421 ttcacttttt cgaaaggtat aaagcccgca gggatacccc ttcagtttag ggcttcttct
     9481 tgcgcatcat gtcttctcca tacgcaagca gttcatcaat cggtcgagaa ttttgcgggt
     9541 cataatgcaa tttgcataga ctcatcgaga gcgtatagtc tcgaccgttc attgcattat
     9601 gagcatcaat gtttttctgt agtctgccag taattttgtc gatatcgtcc cctgcaaccc
     9661 ctatggtaag aatcacgaac gcatcttccc cgatacggga aataatatct gattttcgaa
     9721 aggttgcttt caatataact gcactagcga tcaatgcgcg gtcgccttcc tgagaacccc
     9781 acctgtcatg aatgatctcc aggtcatcca gacctgcata aagcatgacc atccccctcc
     9841 tctcacgctt ggcaagcgcc acctgctgtt cagccacagt aaaaaaccct cgcctgttgt
     9901 aaaggcctgt aagttcatcg ataacagacg tctcgcgcaa gtgcttcagc acttgcgcat
     9961 gctctatcct tatcatgagt tctttgagcg taaagggctt cttgatcaaa tcagatgcac
    10021 cggcttcaat cgcggtgtca tatgtatatt tgttgataaa cccggtcatg atgatggcca
    10081 ccgtgtcagg tctcaatctt tttgcctttt ctataagttc aaagcctttt agtccgggga
    10141 ggacaatgtc tgccaccaaa atatcaaagg agatgttctt tacgagttca agagcagcct
    10201 caccactcct tgctgcttcg catcgatacc cctggagctc aaacccttct tttaacatgg
    10261 gtaacaacgt ttcgtcatcg tcaacgatta atatggaatt tttatcactc attttatcgc
    10321 cttcacggtt ctattagagc ttgagctttt gcatctgcaa agtcaatcct acctgcgtag
    10381 gggaaagaat tttaataaat tgcccagtga gtgttgccac aacgcggcac tactttgcag
    10441 gagtttatca taaatctttg gatgaagggg gcggatgaac cgccccctcg aattgaatat
    10501 taaagtttga aaggattcat gaaaaggata ataagcacca cgacgagggc atagatagca
    10561 agagactcga tcatcgcgag accgatgatg agcgttgtcg tgatttttcc ggatacgccg
    10621 ggattccttg caacaccctc gcaggccttg ctcaggccga taccctgacc aattcctgtt
    10681 ccgagggcag caaggccgat ggcaaggcca cagcctaacg ctgcaagggc ataataatat
    10741 atttttccgg ttgatgcaga agcgggagct gctgcctctt cagcgagcgc caggggggca
    10801 catacccaga gcagagccag ggcaagcgtc attaccgcca caattctttt catcgaaatt
    10861 tcctcctttc ttgagatatt ttgtttaatg ggcctcttca acagacccag cgagataaag
    10921 agaagtcaag agaacaaaaa caaatgcctg tatcaaactt accaggacac ccagaccgag
    10981 aatggccacg ggaacgatta acggtacaag gagtcccagg gcaaacagta acatatgctt
    11041 cgcgatcatg tttccaaaaa gccggacaga gagggttacc ggcctcacga gatgtccgat
    11101 gaactcgata atgaacatca tgggagcaag ccacatgatt ggtccgagga aatgcttgat
    11161 gtacttgaat ccatgcacct tgattccgta gtagtgtgtg gcaagaaaaa ccggaactgc
    11221 catggaagcg gtcatattga tgttgctggt aggggcggta aagccaggaa taaggcccat
    11281 aaaattagag acgagtatat aaagacctaa tgtcccgatg agtgggaaaa aatactttcc
    11341 ccaatggtga cctatgttct cctcagcgag cttatagagg gattccacca tcacctccat
    11401 aaagttctgg acacccttcg gtacaagtgc gagggagcct ctcatgacta atgcaacccc
    11461 gaccaagagg aaggaagcca agaaggcata gctgacgtag tggggcacca cgtggggatt
    11521 gatgatgagg tccatcaata gggattcttc gtgcactcag tcctccaaag tgaaactcag
    11581 agtatcttaa tggagaagat gccaaaagtc aagacaggaa gccattaaaa aaacaaataa
    11641 atctatgaag tattgggaat cgagaagatg cccctagaaa gctatgcgga gcaccctttc
    11701 caccgcaaaa taatccttat gcacagagat gttcctaaac cccaggctct tggctatcgc
    11761 tgccacatct ccggcttcac cgtcacccac ctccatgacc atgaatccgt ccccttccag
    11821 atgctcccct gaactgagga ggatttccct gtagaattta agaccgtcct cacccccgtc
    11881 aagggcttcc atgggctccc aatctttaat ctcgggctgg agggtttcta tctcggtcct
    11941 ccttatgtaa ggggggttgg aaacaactat gtcgaaccgc attcctctca gaggctcaaa
    12001 aagactcccc ttcagaaaag tgacattctc gataccgttc gtcagggcat tctgcctcgc
    12061 gtatttcaag gcccttcctg agatatcagt cccaatcact tctgcccccg gaaactcttt
    12121 agccatggca agggcgagac agccacttcc agtgcaaaga tcaaggacct tcagtctcct
    12181 tttcgtggtg gactgtccag ggtcttttgg tctttgatta atgacttctg actgacgact
    12241 tttgactgtt tttatcactt cctccactac caactctgtc tcgggcctgg ggatgaggac
    12301 accggggccg accttaatgg tcaacccata aaaatccaca tggccagtga tgtactgaat
    12361 gggctccctt agccctctcc gctcaagaac ctttctgagt gtttccacct ggagcggtga
    12421 gagaatcggg ttatcccggt aaagtgcgac cctttcaaga tcaaggcatt gggttaatat
    12481 gatctctgct tccttccggg ggtcttcgat accatatcct cccaggactt ttgaaacctc
    12541 tctcatcccc tcactggccc ttatcacttg agggcctccc tcagcctttc tgtctgataa
    12601 tgggtgatta aggcatcgat aacctcatcg atgttccctt ccagcacctg ttcaagctta
    12661 tggaggctaa gccctattct gtggtctgtc accctgttct gcggaaagtt gtaagtcctt
    12721 atcctctcgc tcctgtcacc tgtgcccacc tgggtctttc tttccagggc acgttccttt
    12781 tcctgcctct ccatttccag gtcatagagc cgtgagcgca ggaccttcag tgccttgtcc
    12841 ttgttcttta tctgggagcg ctcatcctgg cactggacga tcatccctgt aggaatatga
    12901 acgatcctca ccgcagaata ggtggtgttc accccctggc ccccgggccc tgatgcacag
    12961 aatgtgtcta tcctcaggtc cttttcctcc acctttatgt ccacgtcctc tgcctcggga
    13021 agcaccgcaa ctgttgcagc agaggtgtgg atcctgcctg acgcctcggt gactggaacc
    13081 ctctggaccc tatggacccc gctttcatat ttcagcctgc tgtatgcacc cttgccctct
    13141 atggctgcca caatttcttt gagcccacca aggcctgtgg ggctggagtc gatcacatca
    13201 accttccatc tcctggactc cgcatatttc gaatacatcc tgaaaaggtt agaagcaaac
    13261 agtgcagcct cctcaccccc tgtccctgcc cttatctcga ggataacact cttgtcgtca
    13321 cggggatctt tgggaagaag catgatctta agttcttctt cgattacagg tcgcttcact
    13381 cttaattcat caagttccgc ctgcgcgagt tccttcatgt ccccgtcccc gcccttgaga
    13441 agctcc
//
LOCUS       DATKOU010000103         4006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4369617, whole genome shotgun sequence.
ACCESSION   DATKOU010000103 DATKOU010000000
VERSION     DATKOU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369617"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1109
                     /locus_tag="VMU21_04670"
     CDS             105..1109
                     /locus_tag="VMU21_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HUO76852.1"
                     /translation="MLGSLRRFDESEVAQQRHKILRFYDTYGEEATKEAFGVDRKLIH
                     VWRKRLKVKDGHLEALIPHSTKPIRVRRMRTDPRIIGWIRQMRQEHPGMGKEKLKPFV
                     DRYCKEAQIPSVSESTIGKIIKRHKFFYQRSKRVYHDPCSKWMEDRRRKPKRLRIKHA
                     PKHAEFGHFQADSSVKFIDGIRRYMISAIDCTLKFSFSSCYSQLTSSNARDFLGRLEM
                     VYPLPIKSLQTDNGAEFLGDFNASLKHKGIPHYFTYPRCPKINGCVERFNRTLKEEFI
                     NNNLHLIDDLPRFREKLVDYLIFYNTERPHKTLGLKSPIDYLISQGAMSKMIATRTKA
                     "
     gene            1141..1749
                     /locus_tag="VMU21_04675"
     CDS             1141..1749
                     /locus_tag="VMU21_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HUO76853.1"
                     /translation="MNNITEPEERRSLQILTELSRNDSLTQRDLSSSLGIALGLVNSY
                     IKNLMAKGFITVKSAPPRRYAYFLTPKGLAEKTRLTYHLLQDYTRIYREAKSNFRRLF
                     AELSAGGARRVIFAGADEVAEIAYLTLHETNLELSGVVDAEMAGKKFFGRKIRPLRDI
                     GNMSYDFIVVTSYLKREEIYRGLLNNGADKKSVKVIFSHNAA"
     gene            1805..2389
                     /locus_tag="VMU21_04680"
     CDS             1805..2389
                     /locus_tag="VMU21_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HUO76854.1"
                     /translation="MNKEDIEFLINTMPVGVLVFDEKMGVAYCNRNAKKFINRYEIPE
                     EIISVGKRMLNAFASGRMKELFPGEIYIYKKFEDSSSNWTFKLHLYEGIRPFVCVFIT
                     EDALSNKLNLNAIRAQYRLTRREMDVLRRVLNGGKNTEIAEDLQISEQTVKDHLSNIY
                     MKCGAENRFALLSALVNLPGLRVPPDSVPDTPPF"
     gene            3195..>4006
                     /gene="cysQ"
                     /locus_tag="VMU21_04685"
     CDS             3195..>4006
                     /gene="cysQ"
                     /locus_tag="VMU21_04685"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HUO76855.1"
                     /translation="MAESFVTSFLEQLPDIAAEAGAAISDVYHTEFEVSYKVDRSPLT
                     MADQRSHAIILNRLQELLPQIPVLSEEGRDIPYEERRAWEYFWLVDPLDGTKEFVKRN
                     GEFTVNIALIQGNKPVAGLIYIPEKKTYYFAAEGLGAYKLVRDTLSLPRLTYGDLIKK
                     ARRLSSDSRKVQDGRPFTIIGSRSHASKEFEGFIKAMKERYADATILSAGSSLKFCLV
                     AEGAADIYPRFGPTMEWDTAAGQCIVEESGGVVADMSRKRLTYNKESLLNPD"
BASE COUNT         1138 a          953 c          995 g          920 t
ORIGIN      
        1 ctgaaaaagt tacgatacat tttcgacgca gaagtttgat ggatcattac gccagggcat
       61 attctatctt cagttggatc aagggctgga ggagggtaca aggtatgctg ggaagtcttc
      121 ggcgatttga tgagagtgag gtagcacagc agaggcataa gatattgaga ttttacgaca
      181 cgtatggtga ggaagcgaca aaggaggcat ttggggtaga ccgaaagctc atccatgtat
      241 ggaggaaaag gctcaaggta aaggatggcc atcttgaggc gttaatacct cattcgacaa
      301 agccgatacg ggtaagaagg atgcgcacag accctcggat tatcgggtgg atcaggcaga
      361 tgaggcagga gcatcccggg atgggcaaag agaagctcaa gccctttgtc gataggtact
      421 gtaaagaggc gcaaatccct tctgtgtccg aatcaaccat aggaaagatc atcaagcggc
      481 acaagttctt ttatcagaga tccaaaaggg tgtatcacga cccctgcagc aaatggatgg
      541 aggaccgaag gaggaagccc aaaaggcttc ggatcaaaca tgctccaaag catgccgagt
      601 tcggacattt tcaggcagac tcctctgtga agttcataga cggcatcagg aggtacatga
      661 tttcggcaat agactgcacc ctgaagttct ccttttcctc ctgttacagt caattgacaa
      721 gtagcaatgc cagggatttc ctcgggaggc ttgagatggt ctatccctta cccatcaagt
      781 ccctgcagac cgataacggc gccgagttcc tgggggattt taatgcctct ctgaaacaca
      841 aagggatacc tcactacttt acctatccac gctgtccaaa aataaacggc tgtgtggaac
      901 gatttaatag gacccttaag gaagagttca tcaacaataa cctacacctt attgatgact
      961 tgccccgatt cagagaaaag ctcgttgact accttatctt ctacaatacc gaacgacctc
     1021 ataaaacttt gggcctcaaa agccccatag attacctcat ctcacaagga gctatgtcga
     1081 aaatgattgc aactcgtaca aaagcttgaa cttctccttt gcgtatgata tgttcatatt
     1141 atgaacaata tcactgaacc tgaagagcgc aggtcactcc agatactcac cgaactctcc
     1201 cgcaatgact cactcaccca gagagacctc agcagcagcc tcggcattgc gctggggctc
     1261 gtgaactcgt acatcaaaaa cctcatggca aagggattca tcacggtgaa atccgcacct
     1321 cccaggcggt acgcgtactt cctcaccccc aagggtcttg ctgaaaaaac caggctcacc
     1381 taccacctcc tccaagacta tacaagaatt tatcgggagg caaaatcaaa tttcagacgc
     1441 ttgttcgccg aactcagtgc aggtggggca aggcgtgtta tcttcgcggg cgctgacgag
     1501 gtggcagaaa ttgcatacct tacgctccat gagacaaacc ttgagctttc aggggtggta
     1561 gatgcggaaa tggcaggcaa gaaattcttc ggcaggaaaa tcaggcccct cagggatatc
     1621 ggaaacatga gctatgattt catcgtggtc acttcttatc tcaaaagaga ggagatttat
     1681 cgcggactcc tcaataacgg agcggacaag aaaagcgtca aggtcatctt ctcccataac
     1741 gcggcgtgaa gcatactgta aggaagatct gggaagccat ccccagagag atgctatgga
     1801 ggtcatgaac aaagaggaca tagaatttct cataaatacg atgcccgtgg gggtgctcgt
     1861 ctttgacgaa aaaatgggtg tcgcatactg caacaggaat gcgaaaaaat ttatcaaccg
     1921 ttatgagatc cccgaggaaa tcatttccgt tggcaagagg atgctgaacg cattcgcatc
     1981 cgggaggatg aaagaactct ttccagggga gatttacata tacaagaagt ttgaggattc
     2041 ttcgagcaac tggactttca aacttcatct ctatgaagga ataagaccct ttgtctgcgt
     2101 atttattacc gaggacgccc tctccaacaa actgaacctg aacgcaatca gggcgcagta
     2161 caggctcacg aggagggaga tggacgtact cagacgtgtc ctgaacggag gcaaaaatac
     2221 ggaaatagct gaagacctgc agatatcaga gcagaccgtg aaagaccacc tgagcaatat
     2281 ttacatgaaa tgcggtgctg aaaacaggtt cgccctgctc tccgctctcg ttaacctccc
     2341 tgggttgcgt gtgcccccgg acagcgtccc cgacacaccc cctttttagt catagttttc
     2401 cttttccctt tttccggaag attgccgcac tgccgcacgt ctaccgccct gtcctttgaa
     2461 ccaaagcttg ccagcgtagc agcttgcgat cgcacaaccc attgcctgtc gcctggtgct
     2521 tccccttaat gtattgatat tcgggatatt cgtttgtgtg atagtgtttc attaaatttg
     2581 tggcattgga gcgtctgatt catagccgta aaaggcttga ctaaacaaag aggcaaatgc
     2641 tatgttcata taatgaacat aggcttgttt caagaaaagg gcatcctgac tccctaaact
     2701 gatgagaagc cgttggtatg agttcttggc cggtcgtatt gaccagaacg gctgtagcat
     2761 gcccgaacag caatcagccg ccagcaatta gctgttagcg aaaagtaata cgcaactaac
     2821 aatcagtaag cagcagggaa ggccatgaga gattttaagg aattgaaagt ctggcagaaa
     2881 agccatagct taacaatggc agtttacaag gctacaaaga catttccgaa agaggaaatg
     2941 cggcttgaca agccagattc gacgtccgtg ttcttcaatt tcagccaata taacggaagg
     3001 ttgcggaaga gaaggagaag cagcacttgc ccgttttctc cagatagcaa tgggttcggc
     3061 aagttagctg gaacattatc taatgctttc ctgtgatttg ggattcttga aaacacacga
     3121 ctatcaacag cttgttaata aactgtctga agtgaaaaaa atgttagcca catttattaa
     3181 aaagctgggt gctgatggct gagagcttcg taacatcatt tcttgagcag ctgccggata
     3241 ttgctgctga ggccggcgcg gccatcagtg acgtttatca tacagaattc gaggtctcat
     3301 acaaagtaga ccgctcaccg ctcaccatgg ctgaccagcg ctcccatgca ataatcctca
     3361 accgcctcca ggaactttta cctcaaatcc cggttctgtc cgaagaggga cgagacatcc
     3421 cctacgaaga gcgacgggca tgggaatact tctggcttgt tgatcccctt gacggcacaa
     3481 aggaattcgt gaaaaggaac ggagaattca cggtgaacat cgccctgata cagggtaaca
     3541 aacctgtggc gggattgatt tatatcccgg aaaagaagac atattatttc gctgccgaag
     3601 gccttggcgc atacaaattg gtccgggaca ctctttccct gccccggctc acctatggcg
     3661 atttgatcaa aaaggcccgg agactttctt ctgacagcag gaaagtacag gatggcaggc
     3721 cgttcactat aatcggaagc cggtcgcacg catcaaagga gttcgaaggg ttcatcaagg
     3781 caatgaagga gagatacgcg gacgcgacaa tcctttccgc aggcagttca ctcaagttct
     3841 gccttgtggc tgagggagcc gcggacatct acccccgctt tggccccacg atggagtggg
     3901 acacggccgc aggtcagtgc atcgtggaag aatccggagg ggtggtcgct gatatgtcgc
     3961 gaaagaggct cacgtataat aaagagagcc ttctgaaccc tgactt
//
LOCUS       DATKOU010000104         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5528010, whole genome shotgun sequence.
ACCESSION   DATKOU010000104 DATKOU010000000
VERSION     DATKOU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5528010"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VMU21_04690"
     CDS             complement(<1..292)
                     /locus_tag="VMU21_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76856.1"
                     /translation="MYAERISGLKSSAIRDILEVTSRPGMISFAGGLPAPELFPVEQI
                     KKVSQRVLSDYGPSALQYSVTEGILSLRNWIAGEVSSDLGPVASENIIITQGS"
     gene            complement(265..612)
                     /locus_tag="VMU21_04695"
     CDS             complement(265..612)
                     /locus_tag="VMU21_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUO76857.1"
                     /translation="MLFFRESELKAKQLFEGMTLRAVSGDKTMMTFFEFEPHALIPHH
                     RHVHEQITYVIEGEIEFTMDGETKLLKAGDGVIILSNQEHSARILDRPAKALDAWYPI
                     REDYRCTQKESRD"
     gene            complement(700..1899)
                     /locus_tag="VMU21_04700"
     CDS             complement(700..1899)
                     /locus_tag="VMU21_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO76858.1"
                     /translation="METYDIIIVGSGISGMSLAHFCAKSGLTTLVIEKDERIGGTFHS
                     HRFSGEASGFWLELGAHTCYNSYGSLIDVLHDCHILDTVTKREKVGYKMLVNGQLKSI
                     PSRMSFVELLFSAPRLFTQKKTGQTIESYYSKIVGKSNFKKVMAPLFDAVICQKANDF
                     PADLLFKKRPRRKDILKNFTLTHGLQSITDAISVQPKISIIKGKGVQTVTTGNDLFYL
                     TTGDGSTYTAQSLGVATDAIAAARLLKAPFPELSEQLSKIQVNTVESVGVALKRKALS
                     LPPVAGLIAVEDSFYSAVSRDTLKHDTYRGLCFHFREGVLTHEAKLKRIADVLKVNLS
                     QLEEVVSRENRVPSLLLGHERVISETDRLLTGKRLLLTGNYFSGLAIEDCVSRSMTEF
                     LRLKRSV"
     gene            complement(2037..2597)
                     /locus_tag="VMU21_04705"
     CDS             complement(2037..2597)
                     /locus_tag="VMU21_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HUO76859.1"
                     /translation="MDVFEAIRTRRSVRRFKDQAIEEEKVRTILDSVRQSPSWANLQC
                     WRFVVVKDRSIREKISELSYVESFFAPLSYKTNPARKGLAEAPVILVACADPSQSGTL
                     WGQPYYMTDIGIAAQALMLSARGLGLGTVFVGVFEEEQVRNLLNIPSNIRIVGLFPLG
                     YPLEEKTSGPPRKPLTEIVFEEKWKS"
     gene            2816..2998
                     /locus_tag="VMU21_04710"
     CDS             2816..2998
                     /locus_tag="VMU21_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUO76860.1"
                     /translation="MDSLKEKIREIEKGEILRALTETNWVMARAARMLGITERMIGYK
                     IKKYGIRKRGETESNL"
BASE COUNT          736 a          741 c          738 g          800 t
ORIGIN      
        1 gggaaccttg ggtgatgatg atattttcag aagcgacagg gcctaggtca gatgatacct
       61 cacctgcaat ccaatttctg agggaaagga tcccctctgt gacggaatac tggagagctg
      121 aagggccata atctgacagc acacgttggc tgaccttttt aatctgctcc accggaaaaa
      181 gctctggagc tggcagtccc cctgcaaagg agatcatccc cggtcttgag gtcacctcca
      241 ggatgtctcg aatggctgaa ctttttaatc ccgagattct ttctgcgtac acctatagtc
      301 ctcccttatg ggataccacg cgtccaaggc ctttgcgggc ctgtccagaa tcctcgcact
      361 atgttcctga ttggacaata taattacccc gtctcctgcc tttaagagtt tcgtctcccc
      421 atccatggtg aattcaatct ctccctcaat gacataggta atctgctcat ggacatgcct
      481 gtggtgtggg atgagggcgt ggggctcaaa ttcaaagaat gtcatcatgg tcttgtcacc
      541 agagacggcc cttagggtca ttccctcaaa aagctgtttt gccttgagct ctgattctct
      601 gaaaaaaagc atcctcttct ctttttgctc cgattacctt tattcttgca gcatcctttt
      661 ttcagccatc gcaatcgtcc tgcgtacgac attattagat cagaccgaac gtttcaaacg
      721 caggaattct gtcatagacc tggatacgca gtcctcaatg gcaagaccag agaaatagtt
      781 ccctgtaaga aggagacgtt tcccggtgag aagcctgtca gtctcgctga tcactctctc
      841 atggcccagc aaaagagaag gcacgcggtt ctccctgctc accacctcct caagctgtga
      901 gagattcact ttaagcacat cagcgatgcg cttaagtttc gcttcatggg tgagcacacc
      961 ctccctgaaa tgaaagcaga ggcctcggta ggtatcgtgc tttagggtgt cgcgggatac
     1021 cgcagaatag aatgaatcct ccaccgcaat aagaccggcc accgggggca gggagagggc
     1081 cttccttttc agtgccactc ctaccgattc aacagtgttg acctggatct ttgacagctg
     1141 ttcggacaat tcagggaagg gtgctttgag aaggcgcgcg gcggcgatcg catcggtggc
     1201 caccccgagg ctttgggcag tataggtgct cccgtctcca gtggtaagat agaagaggtc
     1261 gttgcctgtg gtcactgtct ggaccccctt gcccttgata atgctgatct ttggctggac
     1321 ggatatggca tcagtgatgg attgcaggcc gtgggtcagg gtgaagttct tcaggatatc
     1381 cttgcggcgg ggacgcttct taaacagaag atcagccgga aaatcatttg ccttttggca
     1441 aatcactgca tcaaagaggg gggccatcac cttcttaaaa ttactctttc cgacgatctt
     1501 tgaataatag gattcgatgg tctgccctgt ttttttctgg gtgaaaagac ggggggcaga
     1561 aaaaagtagt tccacaaaac tcatccgaga agggatggat ttcagctgtc cgttcacgag
     1621 catcttgtag ccaacctttt cccgtttcgt gacggtatcg agaatgtggc aatcatgcag
     1681 cacatcgatc agactgccat aggaattgta gcacgtgtgt gcacccagtt cgagccagaa
     1741 acccgaggcc tcccccgaga agcggtgtga gtgaaaggta ccgccgattc tttcatcctt
     1801 ttctatcacc agggtggtga ggccgctctt tgcacagaaa tgggccagac tcattccact
     1861 tattcctgag ccaactatga tgatgtcata tgtttccata catacctccg tctggattgt
     1921 gattaaatga gaacgtgctt atggtatctc ctttggcatt gttgaacctt aaccgcccgt
     1981 gcattatctt tcatgatgaa gtaattggtt tgactccctc ttcacaatgt tgagggtcag
     2041 ctcttccatt tttcttcgaa gactatctca gtcaggggct tccttggcgg ccctgaggtc
     2101 ttctcctcca gggggtaccc cagggggaat aacccgacga tacggatatt ggaaggtatg
     2161 ttgaggaggt tgcgtacttg ttcttcctca aataccccaa cgaagacggt gcccagccca
     2221 aggccccgcg cagaaagcat gagtgcctgt gcagcgatgc cgatgtctgt catgtagtaa
     2281 ggttgacccc agagggtccc tgattgtgaa gggtctgcgc aggccacaag aatgacaggc
     2341 gcctctgcta atccttttct cgcgggattt gtcttataac tgagaggagc gaagaatgac
     2401 tccacataac tcagttcgct tatcttttct cttatagacc tgtccttaac cacgacaaac
     2461 ctccagcact ggaggtttgc ccatgagggt gactgacgaa cggaatccag gattgtccgg
     2521 accttttctt cttctatcgc ctgatctttg aatctgcgta cgctccttcg cgtccttatt
     2581 gcctcaaaca catccatagg gcctccttat tgtttcaatg acactctaat tataatcttt
     2641 ctttataaac ttataaatca aaatttcctt tcccgccatt tccccaccga ctgatgaatt
     2701 acaaaattgt tttggcatac aaaattgtat ctatggccaa ctttatgttt cctttaaaac
     2761 caattactta gcggctggca tggttttagc attaaaataa ggtcgggtga cgaccatgga
     2821 ttctctgaag gagaagataa gagagattga gaaaggggag atactcaggg cccttaccga
     2881 aacgaactgg gtcatggcaa gggccgcgag aatgcttggg attaccgaaa ggatgatagg
     2941 gtacaagatc aaaaaatatg ggataagaaa gagaggtgag acagagagca atctgtaatg
     3001 gagaagcgca gtttg
//
LOCUS       DATKOU010000105        10003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4757180, whole genome shotgun sequence.
ACCESSION   DATKOU010000105 DATKOU010000000
VERSION     DATKOU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757180"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VMU21_04715"
     CDS             complement(<1..583)
                     /locus_tag="VMU21_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HUO76861.1"
                     /translation="MKSPKSLRNLVLFIGTAIIIGIAAGGFIALSKGVPSVEELMQYK
                     AVPGTKIYADDDTLIGELKIERGVFVPMNRMPEYLVNAVIAVEDSRFWKHKGIDYVAI
                     LRAAVKDVLHVGLKEGGSTITQQLAKITFLTPEKTLQRKVREAALAMKIEKNLSKKEI
                     LELYLNRVYLGHGAYGMEMASKVYFGKSVRDISL"
     gene            816..3089
                     /locus_tag="VMU21_04720"
     CDS             816..3089
                     /locus_tag="VMU21_04720"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HUO76862.1"
                     /translation="MKAVNGLSLTQNALKVLEKRYLQKNEEGKIVETPEGLFRRIAKA
                     IASADVRYGKSQEEVERTETEFYSMLTAMEFLPNSPTLMNAGRKLGQLSACFVLPVGD
                     SMESIFDAVKNAAIIHKSGGGTGFSFSGLRPNGDVVGSTKGISSGPISFMTVFDTATE
                     AVKQGGTRRGANMGLLRVDHPDIQDFITCKDENTKLNNFNISVGLTQTFMTALEEDRE
                     YEFLNPRTRQVSRKVKAKDVFSLIVNHAWKNGEPGIVFLDRLNASNPTPHLGEIESTN
                     PCGEQPLLPFESCNLGSINLSKMVRKVSQQSAVSSQDPAYEVDWERLKDITWKGVHFL
                     DNVIDVNKYPLLQIEEKTRANRKIGLGVMGWADMLIRMGISYNCPEAEKLAEEVMGFI
                     QREGKNASAALAEERGVFPSYEGSIYDGKMRLRNATVTTIAPTGTLSIIAGCSSGIEP
                     LFAVSYVRTVMEGTKLIEVNPYFEEVAKEKGFWSRQLMERIAEKGSLQGFQEIPEEVR
                     KVFVTAHDITPLEHIKVQASFQKYVDNAVSKTVNFPHHATPKDVEEVFLLAHKLGCKG
                     VTVYRDGSREEQVLSTGKTKETAPQPGVAPSAKIMPKKRPEVIRGTTRFMKTGCGNLY
                     VTINEDESGHLFELFTSMGKAGGCASSQAEAIGRLVSLAFRSNIEPEEIIKQLKGISC
                     HQPEWYNGGRILSCSDAIAKALEKYHAKSEKGNGNGHKKGHHEVILMGACPECGGAVE
                     HEGGCAVCHNCGFTKCG"
     gene            3099..3596
                     /locus_tag="VMU21_04725"
     CDS             3099..3596
                     /locus_tag="VMU21_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HUO76863.1"
                     /translation="MSGNNGKGAGRRPSWEEYFIEIAKVVSTRSTCLRRRYGAVIVKD
                     NVIVSTGYNGAPRGSANCVDRGICRRKELNVPAGERYELCEAVHAEQNAIINGSPERM
                     KDGSIYIAGFEENSSAAEGKPCLLCKRMIRNAQIRQVVYLSKDGNIVKVEDVRELDVP
                     PVNAE"
     gene            3809..5095
                     /locus_tag="VMU21_04730"
     CDS             3809..5095
                     /locus_tag="VMU21_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76864.1"
                     /translation="MKIPVIVIAFLMGVLVSPPVIQAEEKKPYAHYWMTIETHNMSIP
                     GMPSGEMSGMEGMMGKMMGMPEFGPTRTLRLQLNSPRPLPPDPDATQDIPLGQNMGKT
                     LPLLIPVHEKAAPERHTPEEREEKIEKPKFRMLIYWGCSEEVRPGQPIVIDTEKMSPA
                     DFGKAFSGRSAMHQSPPSERDGWIYADWPNRKSTLKVPKDSSLLGSHFVHGNYTPDIK
                     FYIDRMRDFMAPVEFSSIKGSTAESIRFQWKEIPTAIGYFATAMAHNQQAGETIFWSS
                     SEVKDSGFGLMDFLTPADVQRFIKDRVVMDPRITSCTIPKGIFKGAEGSMLNFIAYGE
                     EMNVVYPPKPEDPKEPWNPIWTVKVRLKSTGMTMLGAEEREQRQPRKMRGRESQQYKE
                     EMPQEQVQEPPKEQPKTPPEESTIDKINKFKGLFGY"
     gene            5273..5986
                     /locus_tag="VMU21_04735"
     CDS             5273..5986
                     /locus_tag="VMU21_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HUO76865.1"
                     /translation="MEKLSRKMVIELSKTTGEVSGFDLSGLDLSGLDLIAMNFRGSNF
                     RGSDLSKANMSRSVFDETDLRGCDLGSSNFTGASFRNAGIEKCDLTFSTFTGADFSGA
                     NLRGADFTNSKLSGANFRNSDLSGANLQMTDGAMACLAGANLSGTNLRGSSMVGADLS
                     GACLRGAMLHGTDLNAANLSSADMRETKLIETSLIAANLKNADLRSSILKDPDITRAN
                     QEGMKTGALFHEFEGDDVV"
     gene            6091..6387
                     /locus_tag="VMU21_04740"
     CDS             6091..6387
                     /locus_tag="VMU21_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76866.1"
                     /translation="MRRKKTLVLGILILIATMIGGCATTQKQDIESKLISAFAANCKT
                     VELKVEDHDTYMITPTGRVDANGCPITLIMGWREGKIIKEKEVEVCECREEKGR"
     gene            6424..7107
                     /locus_tag="VMU21_04745"
     CDS             6424..7107
                     /locus_tag="VMU21_04745"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HUO76867.1"
                     /translation="MKEKIIFTWSGGKDSAMALHELKRNAGYEIVALLTTITEDYDRI
                     SMHGVRRELLEAQAVSLGIRLEKVLISTQSSNEDYESRMRAALEQYKAKGVSSVAFGD
                     IFLEELRRYREDNLAKIGMKGIFPLWKRRTSDLARDFIHAGFRAVITCVDSEALDKRF
                     AGREFDKAFLSELPSGIDPCGENGEFHSFVYDGPVFKNWLRHTRGEMVLRDQRFWYLD
                     LVPPEIGNK"
     gene            7727..7954
                     /locus_tag="VMU21_04750"
     CDS             7727..7954
                     /locus_tag="VMU21_04750"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HUO76868.1"
                     /translation="MVILVFAVFIIAVVTIFSVQNASPVAVTFLFWNFQASLAIVIFL
                     STLIGVIIGAIVFSVLRRKMSPKKGTDSVIR"
     gene            complement(7976..8392)
                     /locus_tag="VMU21_04755"
     CDS             complement(7976..8392)
                     /locus_tag="VMU21_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8102..8104),aa:Sec)
                     /transl_table=11
                     /product="rhodanese-related (seleno)protein"
                     /protein_id="HUO76869.1"
                     /translation="MKRSLPQLFLVVLCAVLCGLIAREAYSLTKVQRITKEELKALLN
                     DPSVVIIDVRTDWSWRESDKKIKGAVREDPLADVRTWASKYPREKKIVLYCSUPSEHT
                     SASVAQEIMELGFTHVSALKGGWDEWLKAGFPVEKK"
     gene            8449..9228
                     /locus_tag="VMU21_04760"
     CDS             8449..9228
                     /locus_tag="VMU21_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76870.1"
                     /translation="MSIKNHFLKTSESQPIPLPDDSPNHAFQETPMATIAEALLTLAT
                     SRVICSELPGIYTHVRNLREAMGRADSLTQETLLVNLYASLHTAGSLYSPSERVLLSK
                     RAGYSCHAGGLSPLIRAEPFITPDSVVVDLGAGNGLQGLLLQCLSPHRRTLQVELSSE
                     MIRIGRLFQEALGISADRVEWIHDDLVNVSVEAADIVYLYRPARTSPDGRELYRTIAR
                     KLASVPKPLMVFSVADCLGQFLNEQFSLFFTDGHLTCFSKR"
     gene            9309..9839
                     /locus_tag="VMU21_04765"
     CDS             9309..9839
                     /locus_tag="VMU21_04765"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUO76871.1"
                     /translation="MTESVTIALPPEKVFAFLKNVEERLRLNPFYQVLGFEKLTEGNV
                     GVGTRFRIKLLGNDRRAEYESEVLEFVENRKIVTRDTQGRLLLTLTLRETGKGTLLTH
                     DEEFVIPADVLYPHEGEPDLPLWAKVLKGLFSLDRARFTDRDTDRRIEEIKGNLRKNL
                     RVWLFTIKERLEGRER"
BASE COUNT         2641 a         2511 c         2610 g         2241 t
ORIGIN      
        1 gaaggctaat gtccctgacg gatttgccga aatatacctt tgaggccatc tccatcccat
       61 acgcgccgtg tcccagatat accctgttga ggtagagctc cagtatctct ttcttcgaaa
      121 ggttcttttc tatcttcatg gcaagggccg cctccctcac cttcctctgc aatgtctttt
      181 caggagtcag aaaggtgatc tttgcgagtt gttgggtgat ggtgctgccg ccctctttga
      241 gaccgacgtg gaggacatcc ttcactgctg ccctcaaaat ggcgacatag tctatgccct
      301 tgtgcttcca gaaccttgag tcttccactg cgatcaccgc gttcaccagg tattccggca
      361 tccggttcat gggcacaaag acgcctcttt ctatcttgag ctcaccgata agtgtgtcat
      421 catccgcgta tatctttgtg ccaggtactg ccttgtactg catcagctct tccacagaag
      481 gaacgccttt tgagagcgca ataaaaccgc cggcggctat tcctattatt attgcagtac
      541 ctatgaaaag gacgaggtta cgcagggatt tgggagattt catcgaagac cgctgggttt
      601 tgtgtgcatt aaaaattata accccgccaa accttttctg tcaaaaagtt cacaaaaaac
      661 ccacactctt ttcacagcga aagaataaat aaatcaatgg gttcctattt tttacttttt
      721 gtcttgacag atacaatata ttgtggttaa atctaatcct agcaataaaa aatacaaaac
      781 ctagatatta atcatttatc tcccgaggag gaattatgaa ggcagtaaac ggtctcagtc
      841 tcacccagaa tgccctgaag gttctggaga agagatattt gcagaagaat gaggagggga
      901 agattgtgga gacccctgaa gggctcttca ggaggatcgc aaaggccatt gcttctgcgg
      961 atgtccgcta cgggaaatcc caggaagagg tggagagaac cgagacagag ttctactcaa
     1021 tgctcactgc aatggagttc ctccccaaca gccccacact gatgaacgca gggaggaagc
     1081 tgggccagct cagcgcctgt tttgtccttc cggtgggtga ctcgatggag tccattttcg
     1141 acgcggtcaa gaatgcagca atcatccata aatcaggggg gggaacggga ttcagttttt
     1201 cgggcttaag accaaacggt gacgtggtgg gctccaccaa aggcatctct tcaggtccca
     1261 tatcctttat gacagtcttc gataccgcca cagaggcagt gaaacagggg ggcacgcggc
     1321 gcggcgccaa tatgggcctg ctgagggttg accaccctga catccaggat ttcatcacct
     1381 gcaaggacga gaatacaaag ctcaacaact ttaatatatc cgtcggcctc acacagacgt
     1441 ttatgaccgc gctggaagaa gacagggaat acgagttcct taatcccagg acgaggcagg
     1501 tgagcagaaa agtcaaggca aaggatgtct tcagtctcat cgtgaaccat gcatggaaaa
     1561 acggagagcc cgggatcgtg ttcctcgaca ggctgaatgc atccaatccc accccccatc
     1621 tgggagagat tgaatccacg aatccctgcg gtgagcagcc ccttcttccc ttcgaatcct
     1681 gcaacctcgg ttccataaac ctctcgaaga tggtaagaaa agtcagccag cagtcagcgg
     1741 tcagtagtca agacccagct tatgaagtgg actgggaaag actgaaggac atcacatgga
     1801 agggggtcca tttcctcgac aatgtcatag acgtgaacaa ataccccctg ctccagatag
     1861 aagagaagac cagagcgaac aggaagatcg gtctcggggt gatgggatgg gcggacatgc
     1921 ttatacggat gggaatatcc tacaactgcc ctgaggcgga aaaactggca gaagaggtga
     1981 tggggttcat ccagagggaa gggaaaaacg catccgcggc cctggccgaa gagcgtggcg
     2041 tcttccccag ttatgaaggg agcatctatg acggaaagat gaggctgaga aacgcaacag
     2101 tcaccaccat cgcacccact ggaaccctct ccataatcgc aggatgctca tcaggcatag
     2161 agcccctctt tgcggtctct tatgtgagga ctgtgatgga aggcacaaag ctcattgagg
     2221 tgaaccccta ttttgaggag gtggcaaagg aaaagggttt ctggtcaaga cagctcatgg
     2281 agcgaatcgc ggaaaagggc agtctccagg gtttccagga gatacccgaa gaggtgagga
     2341 aggtctttgt tacggcccat gatatcactc ccctcgaaca cataaaggtg caggcttcct
     2401 tccagaaata tgtggacaat gcggtctcca agacggtgaa tttcccccac cacgcaacac
     2461 caaaggatgt ggaggaggta ttcctcctcg cccataagct cggctgcaaa ggggtcacgg
     2521 tgtacagaga cgggtcgagg gaagaacagg tactctccac agggaagaca aaggagaccg
     2581 ctccgcagcc gggggtcgct ccgtcagcca agataatgcc gaagaagaga ccggaggtga
     2641 tcaggggtac cacacggttt atgaagacag gatgcggaaa cctctatgtc acgatcaatg
     2701 aagatgaaag cgggcatctc ttcgaactct tcacctccat gggcaaagca ggagggtgtg
     2761 cgtcaagcca ggcagaggca ataggcagac tcgtttccct tgccttccgc tccaacatag
     2821 agcctgagga gattatcaaa caactcaagg gcatctcatg ccaccaaccg gagtggtaca
     2881 acggcgggag gatactctcc tgctccgacg ctattgcaaa ggcactcgaa aaataccatg
     2941 caaaatccga aaaaggaaac ggcaacggcc acaagaaagg ccatcatgag gtgatactca
     3001 tgggcgcctg tcctgaatgc ggtggcgcag tggagcatga gggcggctgc gcggtctgcc
     3061 ataactgcgg gttcaccaag tgcggttaag gagggtgcat gagcggaaat aacggaaaag
     3121 gggctggccg caggccgtcc tgggaagagt atttcataga gatcgcaaaa gtggtctcca
     3181 cccgctccac ctgcctcaga aggagatatg gcgcggtcat cgtaaaggac aatgtgattg
     3241 tgagcaccgg ctataacggc gctccccgcg gctccgcaaa ctgtgtggac agggggatat
     3301 gcaggaggaa ggagctcaat gtccctgcag gcgagcgata cgaattatgc gaggcggtcc
     3361 atgcagagca gaacgccatt atcaacggct cgcccgagcg catgaaggac ggctccattt
     3421 acatcgcggg gtttgaagag aacagctccg ccgcagaagg aaagccctgc ctcctctgca
     3481 agaggatgat acggaatgcg cagatacggc aggtggtcta cctctccaaa gatgggaata
     3541 tcgtgaaggt tgaggatgtg agggagctgg atgtaccgcc cgtgaatgcg gaataacaac
     3601 tcatcatacc gcattaaacg agtccatgca tgaaactaag attcaaaata ctgtttgggc
     3661 tgctccttac cttttctgta tttactgttg cgtttggcag aggatagaag agttgagtta
     3721 gtgaaaagat gaaggccctg atatatttca caaagagaac atgcttaaaa agtatcactc
     3781 taaatttatt ttggaggaag gagctataat gaaaattcct gtgattgtaa ttgcgttctt
     3841 gatgggagtg ctggtgagtc ctccggtcat acaggctgaa gagaaaaagc cctatgccca
     3901 ctactggatg actatcgaaa cacataatat gagcataccg gggatgccct caggcgagat
     3961 gtcaggcatg gaaggcatga tgggaaagat gatgggaatg cccgaattcg gtccgacccg
     4021 caccttgcgt ctccagttga actctcccag gcctttgccc cctgatcccg atgcaaccca
     4081 ggatatcccg ctgggacaga acatgggaaa gacccttccc ctgctcattc ctgtccatga
     4141 aaaggcagcg cccgagcggc acacccctga ggagagagaa gagaaaatcg aaaagccaaa
     4201 gttccgcatg ctcatctatt ggggctgcag cgaagaggta aggccgggcc agcccattgt
     4261 gatcgacaca gaaaagatga gccctgctga ctttggcaag gccttcagcg gtcggagcgc
     4321 catgcaccaa tctcctccct cggaaaggga tggctggatt tacgcggact ggccgaacag
     4381 aaagagcacc ctcaaggttc ctaaagacag ctccctcctg ggaagccatt ttgtccacgg
     4441 caattacacg cccgatatca agttttatat cgaccggatg cgggacttca tggcgccggt
     4501 ggaattcagc tcaataaagg gcagtaccgc tgaaagcata cgttttcagt ggaaggagat
     4561 ccccactgcc atagggtact ttgccacagc catggcacac aaccagcagg caggcgagac
     4621 catcttctgg tcatccagcg aagtgaagga ctcaggcttc ggccttatgg actttctcac
     4681 ccccgctgac gtgcagaggt tcattaaaga cagggtcgtg atggatccta ggatcacaag
     4741 ttgcactatc ccgaagggaa tcttcaaggg tgctgaaggc agcatgctca atttcatcgc
     4801 ctatggggaa gagatgaatg tggtctatcc cccaaaaccg gaagacccga aggaaccgtg
     4861 gaaccccata tggacagtga aggtgaggct caaatccaca ggcatgacca tgctcggcgc
     4921 tgaagagcgt gaacagagac agccccggaa gatgaggggc agggagagcc agcagtacaa
     4981 agaagagatg cctcaggaac aggtgcagga gcccccgaag gagcagccca aaactccgcc
     5041 tgaggaaagc accatagaca aaatcaacaa gttcaagggt cttttcgggt attaggaaag
     5101 aagacaaatt atacggtata cgtccctctt tttggacaat ccctattatg ttttgtggag
     5161 agggcaagag ttgcactttt aaagccgtaa ctcaccttga aaattaagaa tggtgatgcg
     5221 acgtccgaaa ggtgagaata tttggagaag ggacgatact gattaatctc tcatggagaa
     5281 actttcaaga aaaatggtaa tagagctttc aaaaacaacc ggtgaggtgt ctggctttga
     5341 cctctcgggt ctggacctga gcggcctcga tctcattgcc atgaacttca ggggctcgaa
     5401 tttcaggggt tcagacctga gcaaggcaaa catgagcaga tcagtattcg acgaaaccga
     5461 cctgagaggc tgcgacctcg gcagctcaaa tttcaccggc gccagtttca ggaacgccgg
     5521 catcgagaag tgcgatctca ccttctcaac cttcaccggt gcggactttt ccggtgcgaa
     5581 cctccggggt gcggacttca caaactcaaa gctctcgggt gcgaacttca ggaactcaga
     5641 cctttccgga gccaaccttc agatgacgga cggcgccatg gcgtgcctgg ctggtgcgaa
     5701 cctttccgga accaacctcc gcggttcgag tatggtgggc gcagaccttt cgggcgcctg
     5761 cctccgcggt gcgatgctcc acggtaccga cctgaacgcg gcaaacctct catcagctga
     5821 tatgagagag actaaactca tagagacctc gcttatcgcc gcaaacctga agaacgctga
     5881 cctcaggtcc agcatcctga aagacccgga catcacaagg gcgaaccaag agggaatgaa
     5941 aaccggcgct ctctttcacg aattcgaggg cgacgatgtt gtataggacc ccgcagagag
     6001 aaacgcttgc tagtaaatct catcaaaatt aggacgctta aactggaagg ttttctatcg
     6061 aaacttctga cagagaaagg aggagataag atgaggcgga agaagacact cgttctggga
     6121 atcttaatac taatagctac catgattggc ggatgcgcaa ccacccagaa gcaggacatt
     6181 gagagcaaac tcatttcggc atttgccgcg aactgcaaga ctgtggaact taaggtggaa
     6241 gaccacgata cctacatgat aactcccaca ggaagagtgg acgcaaatgg atgtcccatc
     6301 accctgataa tgggatggag agagggcaag atcattaaag aaaaggaagt ggaggtctgc
     6361 gaatgcaggg aggagaaggg cagatgagtg cgtccgtgca gaagtaggga agctaaccga
     6421 agcgtgaaag aaaaaattat cttcacctgg agtggcggca aggacagcgc catggcattg
     6481 catgagctca aaaggaatgc aggttatgag atagtggcac tcttgaccac cataacagaa
     6541 gactatgaca ggatcagcat gcatggagta cgccgggaac tgcttgaagc ccaggcagtc
     6601 tctctcggca ttcgccttga gaaggtcctc atctcaacgc aatcttccaa tgaagactat
     6661 gaatcaagga tgcgggctgc ccttgagcag tataaggcga agggagtcag ttctgtggcc
     6721 ttcggggaca tttttctgga ggagttgagg agataccggg aggacaattt ggcgaagata
     6781 gggatgaaag ggattttccc tctgtggaag aggagaactt ctgatcttgc cagggacttc
     6841 atccatgcag ggttcagggc agtcatcacc tgcgttgatt cagaggccct ggataaacgg
     6901 tttgcgggta gggagtttga caaggcgttc ctctctgaac tcccttccgg aattgaccca
     6961 tgcggggaaa acggggagtt ccattcattt gtctatgacg gacctgtttt taagaactgg
     7021 ctaagacata cgcggggaga aatggtcctc agggaccaac gcttctggta tcttgacctc
     7081 gtcccgcccg agattggaaa caaatgaact ataaaagctt tataaatcat cgatgcgagt
     7141 tcctgccctg ccataacctg gaggaatggc attcatgcct cttctgttac tgcccgttat
     7201 tcctcctgtc ctgtcccggt gagcatagag tcctgcccac gggactgaag gactgctcac
     7261 cctgcgtcat cccccatacg gagaatggct gggacatcat ccagaaggag ctacaggatc
     7321 aggtattcac tcaatcgccc tgtgtaaaga cccgtctgta agcacagctc ccaaaacaaa
     7381 gaaacttatg aataacgtct gctaccccat tctatatatg agtctctttc ttataggaga
     7441 ggcatccgtc tctcagcaag acaatagctc tcaatttact ctactatggt ctctgcatcg
     7501 aagaacagtc tgttcccatt ttctgtcaat ggtccgctct tgcggatcgg cacataaccc
     7561 tgagtcttgc cgtttgagcc ggatacctct cctaggttta tgaattatgt ctgattgggc
     7621 gcacaccctg ctccgcaatc acttttcaaa aagaaaggcg atagtccacg cgccgatacg
     7681 gtataatggg taatcttaac atcaaggcat gattgggagg gaactcatgg ttattttggt
     7741 ctttgcagta tttattattg cggtagtgac catcttctct gttcaaaacg cgtcccctgt
     7801 tgcggtcact tttctcttct ggaatttcca ggcatccctg gccatcgtca ttttcctctc
     7861 cacactcatc ggggtgatta ttggggcgat tgtcttctct gtcctaagaa gaaagatgtc
     7921 tcccaagaaa ggaaccgata gcgtcatccg ctgagaaaga gctcgccttc tttgcctatt
     7981 tcttttccac agggaaccct gccttgagcc actcatccca tccgcctttc agtgctgata
     8041 catgcgtgaa ccccagttcc ataatctcct gcgccacact ggcgctcgta tgctcagacg
     8101 gtcaggagca atagagaact atttttttct ctctaggata cttcgatgcc catgttctta
     8161 catcagcgag agggtcttca cggaccgcac ccttgatttt cttatcactc tctctccacg
     8221 accaatctgt cctgacatcg ataatcacca ctgatgggtc gttaagaagg gcctttagct
     8281 cctccttagt gattctttgg actttcgtca aggagtacgc ctcccgggca atgagcccac
     8341 agaggacagc acacaaaact accaaaaaca gttgcggtaa tgaccgtttc atcccttcct
     8401 ccttttttca tggtgctgat ttgcctatag tataatatat cactgaccat gagcattaag
     8461 aatcattttc tcaagacatc tgaatctcaa cccatccctt taccggatga ttcccccaat
     8521 cacgcgtttc aggaaacccc gatggcgact attgctgagg cgcttctcac tcttgccacc
     8581 agcagggtca tctgttcaga actgccgggc atttatacgc atgtgaggaa cctcagggaa
     8641 gcaatgggga gggctgattc tttgacacag gagacccttc tggtaaatct ctacgccagt
     8701 ttgcacactg cgggttccct ctattcaccc tctgagcggg tgctcctctc aaagagagct
     8761 ggttactcgt gccatgcagg agggctttcc cccctcatca gggctgaacc ttttataaca
     8821 ccggactctg tggtggtgga ccttggcgca gggaacgggc ttcagggcct gctgctccag
     8881 tgcctttcgc cccatagaag aactcttcag gtggagcttt cttcggaaat gatccgcatc
     8941 ggccggctct tccaggaggc actgggaatc agcgctgacc gtgtggaatg gatccatgac
     9001 gatctcgtaa atgtgtccgt ggaggctgct gatattgtct atctttaccg tccggcacgg
     9061 acctctcccg atggccgtga gctctaccgg accattgccc gcaaactcgc atcagtgccg
     9121 aaacccctca tggtgttcag cgttgccgac tgtcttggac agttcctcaa tgaacaattc
     9181 tcactctttt ttaccgatgg ccacctcacc tgtttctcga agaggtaggg gtgacgtttc
     9241 ggtacagatc agtgatcccg cacctccgtg gaaagaatag gatataaagg agtctgatca
     9301 tacacaggat taccgagtca gtaaccattg ctttaccgcc ggagaaggtc tttgcctttc
     9361 tcaaaaatgt cgaggagcgc ctgagactca acccttttta ccaggtcctg ggctttgaaa
     9421 agctgacaga ggggaatgtg ggagtaggca cacgattcag aatcaaactg ttaggtaatg
     9481 acagaagggc agagtacgaa agcgaggtgc ttgagtttgt agaaaacaga aaaattgtga
     9541 cgagggatac ccaaggcaga cttcttctta ccctcaccct cagggaaacc ggcaaaggca
     9601 cgctcctgac ccatgacgaa gagtttgtaa ttcccgctga cgtcctgtat ccccatgagg
     9661 gggaacccga tcttcctctc tgggcaaagg tgcttaaagg cctcttctcc ctggacaggg
     9721 cacgatttac tgacagggat acggacagaa gaatagagga gataaaaggg aatctcagga
     9781 aaaacctcag ggtgtggctc ttcactataa aggagcggct ggaaggcagg gaaagataaa
     9841 ggagataaaa ggggacggaa aaatctccat ccccttttat gcattccctc ttatttcagc
     9901 ttaagggtat tacacgctgc cttatagtca tcagcggtca acttctcgtg catcttggcg
     9961 gccttgcctt ccttcactcc cttcatcagg gcatcacagc tct
//
LOCUS       DATKOU010000106         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2832489, whole genome shotgun sequence.
ACCESSION   DATKOU010000106 DATKOU010000000
VERSION     DATKOU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832489"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2398)
                     /locus_tag="VMU21_04770"
     CDS             complement(131..2398)
                     /locus_tag="VMU21_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUO76872.1"
                     /translation="MSIMDFLESLEKDSRFMLQLAGHRYIPPIQPRHMKLEVKDVLRE
                     VLAREGVGRFWSHQAEAIDLIRQGENVVVMTPTASGKSLIYNIPVIESLMDNHDTKAL
                     YIFPLKGLEQDQVKNLNEFFNSVHINPPKQKRGSRSHLEAAEVYDGDTTSYRRKQIRE
                     SVPSVVFTNPDMLHLAINPFHAKWEPFLRGLRYVVLDEVHTYRGVFGSHMAHVVRRLR
                     RICHHWGATPQFIACSATIANPQKLTEDLVGLPFQTVLESGAPHSGIHFMFLSPAESP
                     YTEATWLFIQCVKAGLRTIVFTKARKITELIYRWTIDRAPELKEKISPYRAGFLPKER
                     REIEQRLFSGDLLGVVSTSALELGVDIGGLDVCILAGYPGSISSTWQRAGRVGRHGQE
                     SLIFMIGLQDALDQYFIRNPDAFFGKNHEAAVIDTANTHILKQHLPCAASEVYLKEND
                     TVYDVTSLKPVIDECTNEGLLSQGKTGDIWFSRNRMPHRDVGIRAIGESFPIMDDSGK
                     SIGELSGYRVFLDAFPGAIYLHRGRQYMVHKLDIERKKVFCREVDVLYYTQALSKEET
                     EVREGKERKNLQKFTVNWGTLRMTNRVTGYERKNIFDRTKISRHDLEMPEYLFDSEGL
                     WITIDKDTQTSLESHGYDLAGSLHAAEHASIACIPLFALCDKGDIGGLSYELYPQFKA
                     PALFIYDGFEGGIGLTKRAFDVIEEWFRAALMVITECPCEHGCPSCVQDPQCGSGNQP
                     LDKEGAKFLLKRWVG"
BASE COUNT          579 a          701 c          614 g          670 t
ORIGIN      
        1 cacatcaaag atggggaata tccgcctacg ggaagtgaca ctaaaaagcc atataaaaat
       61 atcttgactg gaataaacaa ggaagaaacc gaaaactttt ggagtactcc tagacatgta
      121 gttttctttc ttaccctacc catctcttga gcaaaaactt cgctccctcc ttgtccagtg
      181 gctgatttcc tgaaccacac tgagggtcct gaacgcatga ggggcatccg tgctcgcagg
      241 ggcattctgt gatcaccatg agggctgccc tgaaccactc ctctatgaca tcgaaggccc
      301 tcttcgtgag accaatgcct ccttcgaacc cgtcatagat gaatagtgcc ggtgccttga
      361 actgcggata aagctcgtag ctgaggcctc cgatgtcacc tttgtcgcat aacgcgaata
      421 aggggataca cgcaatggag gcatgttccg ctgcatggag gctccctgca agatcgtagc
      481 cgtgggattc caatgaggtt tgggtatctt tatctattgt tatccacagc ccctccgaat
      541 cgaagagata ttcgggcatc tccaggtcat gtctcgatat ttttgtcctg tcaaagatat
      601 tcttcctttc ataccccgtc actctgttgg tcattcggag tgttccccag tttacggtga
      661 acttctgaag attctttctc tccttcccct ccctgacctc tgtttcctcc ttgctcaatg
      721 cctgggtata gtaaaggaca tccacttccc tgcagaagac cttcttcctc tcgatgtcca
      781 gtttatgaac catgtactgt ctccccctgt ggaggtagat tgctccggga aaagcatcca
      841 ggaacaccct gtagccgctc aactcaccga tgcttttccc tgaatcatcc atgatgggga
      901 aggactcacc aatggccctt atccccacat cgcgatgggg catcctgttt cttgaaaacc
      961 atatgtcccc tgtctttccc tgactgagaa gcccctcatt cgtacattca tcaatgactg
     1021 gcttgagcga tgtcacatca tagacagtgt cgttctcctt caggtacacc tctgatgcgg
     1081 cgcacgggag atgctgcttg aggatgtgtg tattcgcggt gtcgatgact gctgcctcat
     1141 ggtttttacc gaaaaaggca tccggattcc ttatgaaata ctgatcaagg gcatcctgga
     1201 ggcctatcat gaaaatcagg gattcctgac catgtcttcc cacccttccc gctctctgcc
     1261 aggtgctgga aatggagccg ggatagccgg caagaatgca gacatccagt ccaccgatat
     1321 ccacacccag ttccagtgca ctggtggaga cgacaccaag gaggtcaccg ctgaagagcc
     1381 tctgttctat ctccctcctc tccttgggaa gaaagcccgc acggtagggg cttattttct
     1441 ctttcagttc cggggccctg tcaatggtcc accggtagat tagttcggtg atcttccttg
     1501 cctttgtgaa cacgatcgtc cttaggcccg ctttcacgca ctggatgaaa agccatgtgg
     1561 cttccgtata ggggctttca gcgggactga ggaacatgaa gtgtatccct gaatggggtg
     1621 cgccgctttc cagtactgtc tgaaaaggga gccccacaag atcctcggtg agtttctgag
     1681 gattggcgat ggtggcagaa catgcgatga actgcggggt tgcgccccag tggtggcata
     1741 tcctcctcag ccttctcacg acatgggcca tatgggagcc gaagacccct cggtaggtat
     1801 gcacctcatc cagcaccaca tacctgaggc cccttaaaaa gggctcccat tttgcatgga
     1861 aggggtttat cgccaggtgg agcatatcag gattcgtgaa gaccacacta gggacagact
     1921 ctcttatctg cttcctcctg taggatgtgg tatcaccatc gtacacctca gcagcttcaa
     1981 gatgcgagcg tgagcccctt ttttgctttg ggggattgat atgcacggaa ttgaaaaact
     2041 cgttcaggtt ctttacctgg tcctgttcaa gacccttcag agggaatatg tagagcgcct
     2101 ttgtgtcatg gttatccatt aaggactcga tcaccggtat gttatagatc aggctttttc
     2161 cgcttgcggt gggagtcatc accaccacat tctctccctg cctgatgagg tcaatggcct
     2221 ctgcctggtg gctccagaac ctcccgacac cctcacgtgc gagcacctcc ctcaacacat
     2281 cctttacttc gagcttcatg tgtctcggtt gaatgggtgg aatatacctg tgccctgcga
     2341 gctggagcat gaacctgctg tccttctcca atgactcgag aaaatccatg attgacatga
     2401 ggtcattata caataaccgc tgggagaggt ctgtgaacaa gcaatgccga tctgttgcgg
     2461 cgtattaagc tctctcttct ccatccccct atgcataaga atgcgtatca cttcagcagc
     2521 acattcgcaa ggagaaattc cgcagcggtc tcagtagaga gagg
//
LOCUS       DATKOU010000107        44555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4757712, whole genome shotgun sequence.
ACCESSION   DATKOU010000107 DATKOU010000000
VERSION     DATKOU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 44555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44555
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757712"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1488)
                     /gene="purF"
                     /locus_tag="VMU21_04775"
     CDS             complement(67..1488)
                     /gene="purF"
                     /locus_tag="VMU21_04775"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HUO76873.1"
                     /translation="MNALLRHMFHDIHEECGVFGVFNHPEASNLTYLGLYALQHRGQE
                     GAGICSSDGKQLYLEKSMGLVADIFSEKRLKRLPGYLAIGHNRYSTAGSSVLKNVQPI
                     MANFALGPLALAHNGNLVNAAELRKELEDEGAIFQSTSDSEVIVHLIAHAKGDNPYDR
                     IIQALSRVSGAFSLLILREKELIAVRDQYGVRPLCLGQVDGAYVVASETCALDLINAS
                     YIRDVEPGEMLIIDENGLRSVRALPGTRKAHCVFEFIYFSRPDSYIFGYQNVNEVRKE
                     FGRQLARELPVDADIVIPVPDSGVPAAIGYSEESGIHFDFGLIRNHYVGRTFIEPKQS
                     IRHFGVKIKLNPVKQLLEGKRVVVVDDSIVRGTTSKKIIKMIREVGGAKEVHMRISSP
                     PTIGPCFYGIDTPTRQELIAYTHQIEETRRYITADSLAYLTLEGLKKIVPDSGDFCRA
                     CFDNEYPISFPGEHLEQMELIFR"
     gene            complement(1510..2193)
                     /locus_tag="VMU21_04780"
     CDS             complement(1510..2193)
                     /locus_tag="VMU21_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HUO76874.1"
                     /translation="MSRIIVDIETVGRDFGSFDKTTQDYLLKWAETEEDIQEVKDSLS
                     FYPLTGEIVAIGMLNPDTMKGAVYFQAPQNPLLPFEEDAIHFETGTEPGIIQKFWELI
                     KGYDQIITFNGRSFDCPFIMIRSAVHKIKPSRDLMPNRYSGPHIDLFDQLTFYGASRR
                     KFSLDMWCRTFGIKSPKSEGITGYEVKDLYSSGKYLEIAKYCFGDLRATRELLAYWEK
                     YIRFPSEKS"
     gene            complement(2286..3590)
                     /locus_tag="VMU21_04785"
     CDS             complement(2286..3590)
                     /locus_tag="VMU21_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HUO76875.1"
                     /translation="MITKRKMLITLALVFTIAVSGIAVGRWSVGLVNAEVEGYESIKI
                     FTEALSIVKKNYVEETKTKDLVYGAIKGMVNALDPHSGFMNPEAYKEMQVDTKGEFGG
                     LGIQIGIKDNVLTVIAPIEDTPAYKAGIKAGDRIVKINDEITRDMGLLDAVNKMRGPK
                     GTSVKITIMREGWNEPKEFTLIREIIKIKSVKYKVLEEGIGYIKLTQFQEQTASDLSK
                     ALSQLTSENVNSLVLDLRNDPGGLLNSAVDVTSQFLPPGKLVVYIKDRAGEKNEYRTG
                     GNYTYYDKPSMVVLVNQGSASASEIVAGALKDWRRAVILGAQTFGKGSVQSVIPLSDG
                     SGLRLTTARYYTPSGTSIQSTGITPDIVVKIEAKNGIKEHTVVREKDLERHLKNEKEE
                     EKSKTEQEMAPLEVSEKDDVQLQRAIDLLKTWKVFKELPKAS"
     gene            complement(3608..4804)
                     /locus_tag="VMU21_04790"
     CDS             complement(3608..4804)
                     /locus_tag="VMU21_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUO76876.1"
                     /translation="MHNAQSNIVGVRYFKHLMWYAAFLLFFFPWSFFSGLLFPDIVSA
                     EDAKEQYQKLQKEMETRKQRLEHAKKRERSVLEDLDHVSRSLSTIEAELKKQQQRIRE
                     TESEIGRVEHEISVIEGELEKERAWLKRRLQAMQRYGQSGDILFLLAATDDLAALVRR
                     WKYLERIAMKDRKIIEQFAENLRSLDRKEKQLQGLRAELKKGQERLKPIEASLSETKK
                     DKEIILASVRKEKASQEKLLKELQEASKRLLDVLRKLEEKDTYEAKGFSGLRGNLIWP
                     VNGKVAIPYGSQRDPKFNTPVFRNGIYIKSDEDSVKAVYAGKVVFADWFKGYGNLVII
                     NHGEGYHTLYGNLSETFLKVGDIIKIKDAVGKVGESGIVNAPSLYFEVRYKGKPLDPM
                     QWLKRK"
     gene            complement(4815..5684)
                     /locus_tag="VMU21_04795"
     CDS             complement(4815..5684)
                     /locus_tag="VMU21_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HUO76877.1"
                     /translation="MPYSLRIALQSLWHEKWINFLSMLTIAMGLLVITLMVSTIYNIN
                     LFARKLPERFFIVAYLGDRVNEQQAQDIIASLKERNGIEKVKYISKAEALKELRTSLK
                     DAEYVLEGLNENPLPASIEIRFKKEAMGPESVKTFVNSMKKIEGIEDVQYGEKFLLSI
                     HSLKTAMETIGLILTLVTTSGIIFICYSTVKILFYRRKEEIETLKLLGATRAFIRTPF
                     VIEGAVIGVVGGVLSMIVVITFYLSVFRQLSATLPIVRTVVFPAELFLPLPLIGLFLG
                     ITGSFIAMGRIKF"
     gene            complement(5688..6350)
                     /gene="ftsE"
                     /locus_tag="VMU21_04800"
     CDS             complement(5688..6350)
                     /gene="ftsE"
                     /locus_tag="VMU21_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HUO76878.1"
                     /translation="MIEFQEVSKFYDTVTALRNINLLIEKGEMLFLTGPSGAGKSTLL
                     KLIYAAEMPDSGRISVATWDTARLNGSTIPKLRRTIGIVFQDFRLLLNRNVFDNIALT
                     LRVRRVPEREVKAQVLDALKMVNLRHKADNLPSALSGGEQQRVVIARAIVGNPTILLA
                     DEPTGNLDPDTAAGIMRIFREINAKGTTILIATHNPNLFKDTGLRILRLDNASLVREE
                     KG"
     gene            complement(6347..7312)
                     /locus_tag="VMU21_04805"
     CDS             complement(6347..7312)
                     /locus_tag="VMU21_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HUO76879.1"
                     /translation="MGERKGSPSPGGTSQALATRDAYGAALLELGKKRGDVVVLDADL
                     SGSTKTAKFAKAFPERFFNLGISEQDMIGTAGGFSLAGKLPFASTFAVFETGRAWEQI
                     RQTICYSNLNVKLVATHAGITVGEDGASHQALMDIALMRVLPNMTVIVPADGRETEQV
                     IYSIADYEGPVYVRLGRMKVPLVTPESYRFQLGKAHIFHLGKDATIIATGIMVSASLE
                     ARQILKSEGLDVGVINISTIKPLDAEAVLRAAESSGLIVTVEEHSVIGGLGSAVSEIL
                     SEKHPVKVRRIGVGDTFGRSGKPEELLEFYGLTPDAIVNTIKTSL"
     gene            complement(7312..8142)
                     /locus_tag="VMU21_04810"
     CDS             complement(7312..8142)
                     /locus_tag="VMU21_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HUO76880.1"
                     /translation="MSRITATRDVGLLRERAKEVRREIVKMLTEAGSGHTGGSLSAAD
                     IVAALYFYKMRHRPEEPEWRERDRFVLSKGHAAPVLYAALALSGYFDRELLKTLRRID
                     SPLQGHPSSKMLKGVEISTGSLGQGLSIANGMAMGLRMDGLGSRVYCLLGDGELQEGQ
                     VWEAAMTAVHYGIDNLCAIVDNNGLQIDGFCCDVMKIEPITEKWKAFGWHVMDIDGHA
                     MEQIVDALDEAERVKGRPSVIVARTVKGKGVSFFEGKAEYHGIAPTREERDRALEELK
                     "
     gene            complement(8139..8537)
                     /locus_tag="VMU21_04815"
     CDS             complement(8139..8537)
                     /locus_tag="VMU21_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76881.1"
                     /translation="MLIGYNTNISHKGRQYHVQTEDSGLKSHSIVTLLYHQGAILRSV
                     KTSYAHIIGSPDFEKQLKNLMKLQHREAIKELLTGKGEAKLPGDKQEPIPQPGEKSAV
                     MPEKSESKNKAPKSLDEILLEHISKKVKNG"
     gene            complement(8659..9225)
                     /locus_tag="VMU21_04820"
     CDS             complement(8659..9225)
                     /locus_tag="VMU21_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HUO76882.1"
                     /translation="MSAPFMVSHTAGLVLAVILGFFFGLFLERGGLGDPHKLTGVFYL
                     TDFSVPKVMFTGMLVAATGLYLLSDLKVLDMNRIWIVPAFFWPQLVGGALFGIGFLFS
                     GYCPGTAVAGFATGRLDALVTMGGIGAGSLVFAVVYPAIEGFYFSSPMEGATIPSVLG
                     ISHWVVMAFVYLMAGGMFYVMERYERKR"
     gene            complement(9222..9731)
                     /locus_tag="VMU21_04825"
     CDS             complement(9222..9731)
                     /locus_tag="VMU21_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HUO76883.1"
                     /translation="MKNDNFWSPYIAGAGLGLTLLATFVIVGWGLGASSAFSLLTGVG
                     LDKLSPAYAHKITYFSQYLNVEAPLLNWVLFEIMGLFVGALVGSVAGGNFRVRFDKAE
                     HMSTGTRLMSAFGGGILIGFASRLARGCTSGVALSGGAQLAIAGWVFVIAMFVGGFAA
                     AALFRRLWS"
     gene            complement(9978..11243)
                     /locus_tag="VMU21_04830"
     CDS             complement(9978..11243)
                     /locus_tag="VMU21_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUO76884.1"
                     /translation="MRKLFLLLLVLVMVAVVTPPAMATNGDNLIGIGPISRGMGGVGI
                     AAPQDAISAVFANPAAMCFGPYCPGSEVNFAGTLFMPNVTGSVTTSGQTVNADSDKKV
                     YAIPAIGLSVPITTDWRFGLSAYGVSGLGVDYRNTGLAGTFPAPPFPAGTPLIAGSYT
                     QLQAMKFAPSIAYAVNPNLSFGLNLQIDYSSLDLGSGTSFNYGVGVQLGALYKPLDML
                     SLGLTYVTPQEVNYKNVLNLGGTVGSLKLASPQQIGLGIAVEPIKDTLLVEVNGKWIN
                     WANAKGYDDFDWKDQWVFGIGAQYKPIKPLALRIGYNYANNPVREHSNFVGLTPAGPN
                     MISVQGNNMPTYYYETFRIIGFPAIVKQHITFGAGYEISKRFAVNAGYTHAFKEDISE
                     SGTNFAGQPVTLKSSLYEDSLEFGFTWRF"
     gene            complement(11412..11651)
                     /locus_tag="VMU21_04835"
     CDS             complement(11412..11651)
                     /locus_tag="VMU21_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HUO76885.1"
                     /translation="MDKSKANLAVDLKGLACPMPVLKTKKALDSLQPGQVLLVEVTDK
                     GSKADIPAMLRRTGNELVEMDEKDNVFSFLIKKVK"
     gene            complement(11701..12180)
                     /locus_tag="VMU21_04840"
     CDS             complement(11701..12180)
                     /locus_tag="VMU21_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HUO76886.1"
                     /translation="MEEKKKRMALIASKGTLDMAYPPLILASTAVAMDVEVEIFFTLY
                     GVDIVNKKKYHSLQVAPIANPAMPSPVPFPNILGVLPGMTGMATMMMKGMIKKINWPT
                     IPELLHVCLESGVKMIACTPTLEMTGLSKEDLVEGVEVAGAAEFLDFALDANISLFI"
     gene            complement(12330..13073)
                     /gene="queC"
                     /locus_tag="VMU21_04845"
     CDS             complement(12330..13073)
                     /gene="queC"
                     /locus_tag="VMU21_04845"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HUO76887.1"
                     /translation="MGNKAVALLSGGIDSSTALAIFLAEGHDVYALSFDYGQRHNREL
                     ESAKRVAQSLGVARHLIIKVDLREIGGSALTSEMEVPKSGIGKKADIPSPVPVTYVPA
                     RNTIFLSFGLAWAEVLEAEDIVIGANTVDYSGYPDCRPAYLKAFEDMANLATKASVEG
                     KLRFRIKAPLLFLTKKEIIRKGKDLGLDYSLTWSCYDPQPRKTQSEDFRLSNFCTIYV
                     PCEKCDSCIFRAKGFAEAGMKDPLLQPRI"
     gene            complement(13143..14231)
                     /locus_tag="VMU21_04850"
     CDS             complement(13143..14231)
                     /locus_tag="VMU21_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUO76888.1"
                     /translation="MALEGSLTDFGLADILQLIYFQKKSGILSLSGHRDKVQLIFSEG
                     NVVSAESRKRMEDNRLGKILLKKGLIKGEDLRSTLEEQKRTGARIGDVFLRKGLVTKE
                     DIKETLISQMTETVVQLFSWKEGTYEFQSQEVSVSKDLALTLDTQHLLMEGLRIMDEW
                     SLAEGKILPETVFTITGKTGPALTTEEETILNFVDGENDVSIIIDLCGMEDFQASRIF
                     LSLLEREIIEPVKAAPVKAERVAIPVVHRERPLARFFVPAALCLTLIISLIAAVFQGT
                     GTQRGFSLSWNGDSFARLRTVSTIEELRFMAEVYRYRNGSYPSQINQIGDARDSWGRP
                     FIYSVESGNVMILSTGPDGKAGTADDLY"
     gene            complement(14222..15919)
                     /locus_tag="VMU21_04855"
     CDS             complement(14222..15919)
                     /locus_tag="VMU21_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO76889.1"
                     /translation="MAKFTVAHSKSKTFTVWIVLLGIWGFVHGAGPCEGVEDLYQSRL
                     DRGLSTTEPYSYLFIAKAHQEPANARDFLKMAKQYSPDLPAVYFALASERLFMGDIFQ
                     AIDSFREGIKAYGRNFWWAFTLLGLLYSSILISLVFSLVLILIIRIFMDAGLVSHDGM
                     EDQRRLVLLLVPVILSLFGPIALIAGIFFLIGGYFKKENKVVVYAALLFFLFAPTLLN
                     VEGKFFSAPSPRLRAIVEVNEGKNNRYALWAIKGREDFASRFSHTLALKREGNFNEAI
                     ESYLSLMGDSSRSGPRLFVNLGNAYYAVQNRAAAEDSYQKALQRSPLPSAYYNLSQLH
                     REMLDFAKGDEYFLEAAKLNPEAVSKFTSRSGTGPNRFVVDEMLPPSALWEYALGMGN
                     GSLYRFPLLITLMAFLMIPGFYLLNKGTGHRAQRCKRCSAVFCSRCSRVITWGEMCPQ
                     CYTSLTKMDEVDSKERIARLLSVYESKTRRRKRAKILSCVLPGAGQIYSGKILLGLLL
                     LWPFSFSLVLFLLNNLPTVGIWPFSHAWITPLAVMLMAFTYLFSILHIGRGIRKGWL"
     gene            complement(15919..16506)
                     /gene="plsY"
                     /locus_tag="VMU21_04860"
     CDS             complement(15919..16506)
                     /gene="plsY"
                     /locus_tag="VMU21_04860"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HUO76890.1"
                     /translation="MNVFSLILFAFLLGSIPFGVVVARVYGVNLKKVGSGNIGATNVL
                     RAMGKGPALLTLVGDVLKGSLAVVVGKYFFQSPSLEGIMGLSAIVGHNFSLFLRFRGG
                     KGVATSIGVLLIYSPKVGVLTVILWLIVILVTRYSSLGALVSFGVLPLGVYVLDYTQE
                     KLIVSILIASLLVLRHTDNIKRLLQGTEPKIGKRV"
     gene            complement(16503..17027)
                     /gene="pgsA"
                     /locus_tag="VMU21_04865"
     CDS             complement(16503..17027)
                     /gene="pgsA"
                     /locus_tag="VMU21_04865"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HUO76891.1"
                     /translation="MRLNLPTLITLSRIVLIPFFVLVAPDRPFLGALIFGVASITDFL
                     DGYLARRTGQITKFGIILDPIADKFLVISALILLVDMARLSAWIAITIIVREFLVTGL
                     RAVALSKDIVIPAEMGGKLKTAFQITAILCLIISTDIFGIDLYDVGILSIWIALILSI
                     VSGVQYTVSFWKKI"
     gene            complement(17017..17556)
                     /locus_tag="VMU21_04870"
     CDS             complement(17017..17556)
                     /locus_tag="VMU21_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HUO76892.1"
                     /translation="MKRGEKELQGIAKDVKLLILDVDGVLTDGSIILDNEGNEFKIFN
                     VRDGHGIKMLVKAGIWVALITGRHSKVVERRARELGITEVYQRCHIKTVAFDHIIEKY
                     GLTEKEVAYVGDDIVDIPIFKRVGVPIAVSDAADEARDHAAVVTRHRGGRGAVREVCD
                     FLLKAKGLWESVTKEYFEA"
     gene            complement(17557..18522)
                     /locus_tag="VMU21_04875"
     CDS             complement(17557..18522)
                     /locus_tag="VMU21_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HUO76893.1"
                     /translation="MNILDIARKVLKTEADAVFALIEKIDSSFEGAVDLIYRSRGRVV
                     VTGMGKSGLVGKKIAATLASTGTPAFFLHPAEATHGDLGMVTSDDVVIAISNSGETEE
                     LVCLLPFLKRFDVGLISVTGKNDSTLAKTADVNLDVSVQEEACPLGIVPTASTTAAMA
                     MGDAIAVALLIKRGFKEEDFAFFHPHGSLGKKLFIKVKDLMHTGDALPVVSPETPMLR
                     AVMEISSKRLGVTIVADAEKKILGIITDGDLRRGIEKKGAAFFDLKVGEAMIPHPRMI
                     SEDELAARALSLMENYSITSLVVPDGEGRAKGVIHLHDILKQGIV"
     gene            complement(18542..19075)
                     /locus_tag="VMU21_04880"
     CDS             complement(18542..19075)
                     /locus_tag="VMU21_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76894.1"
                     /translation="MRGSVTKVEYGREKGQNIHTFLIISWTILLNVFFFLSVMGAESK
                     PADDAPYFTNEDVKKYRQPADDGNSAVKHDKAGDKGETAKKGESQKEQEYWCKKATSY
                     RRKIEKNQEEVLNKEKVLTDLKESGGKKKRVPRRSSRNREGNSKSQNETLRTWKMMPT
                     GGLSLQDGYAASLIGKW"
     gene            complement(19079..19897)
                     /gene="kdsA"
                     /locus_tag="VMU21_04885"
     CDS             complement(19079..19897)
                     /gene="kdsA"
                     /locus_tag="VMU21_04885"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HUO76895.1"
                     /translation="MVRKITIAGAVVGGGAPPLIIAGPCVIESEEIAFESAKRLKEIC
                     SAASLHLIFKSSYDKANRTSLESFRGPGMERGLRILANVKAKLDIPVISDVHSVHEIK
                     SASEVLDALQIPAFLCRQTDLILAASETGKPVNIKKGQFLAPWDVRNIIEKFTSTGNH
                     NLLISERGTTFGYNNLVVDFRGLPIMRSYGYPVIFDVTHSLQLPGGQGSSSGGQGEFA
                     EPLARAAVAIGVDGLFMEVHPEPERALCDGPNMMKLDDLPRMLATLKAIDALVK"
     gene            complement(19901..21559)
                     /locus_tag="VMU21_04890"
     CDS             complement(19901..21559)
                     /locus_tag="VMU21_04890"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HUO76896.1"
                     /translation="MAKFIFITGGVVSSLGKGIAAAAIGALLEARGLKVTIQKLDPYI
                     NVDPGTLSPFQHGEVFVTDDGAETDLDLGHYERFTRMRTSQANNYTTGKIYYNVITKE
                     RKGDYLGDTVQVVPHLTDEIKQAIKSVSGSNDVVIVEIGGTIGDIESLPFLEAIRQMR
                     YDVGRENVMYVHLTLVPYIKAAGELKTKPTQHSVRELRAIGIQPDILLCRTDRPIPPE
                     AKKKIAIHCNLDGDTVINALDVETIYEVPLALSEEGLDQQIISKLKLPSRERNLELWK
                     DIVRKLKEPRNEVSIAIVGKYVGLKDSYKSLMEALVHGGIANDAKVDLLWVDSEEIEV
                     HGPDKFLSEVDGILVPGGFGYRGIEGKIEAVKYAREKRIPYFGICLGMQCAVIEFARN
                     VCGLDANSTEFNMKIKDPVIYLMERWYDFRRGKIEERSEGCEKGGTMRLGAYPCVLEQ
                     NTHAYRAYEVKEISERHRHRYEFNNAYKGICTRNGMKISGTSPDGELVEIVEIEDHPW
                     FLGCQFHPEFKSRPTEPHPLFKAFIGAALKEKRSLFPYVAEERG"
     gene            complement(21538..22296)
                     /gene="kdsB"
                     /locus_tag="VMU21_04895"
     CDS             complement(21538..22296)
                     /gene="kdsB"
                     /locus_tag="VMU21_04895"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HUO76897.1"
                     /translation="MSAVIVIPARYESTRFPGKPLALLKGIPVIQHVYENSKGAATAE
                     EVIVATDSETVFEKVLSFGGRVVMTSAGHVSGTDRVAEIARSLSCDIIVNVQGDEPLI
                     RSEMIDDAIGLLSDSRADIGTLVKEIERPEEVVDSNVVKAVFDRDGFALYFSRAPIPY
                     HRDAWKDLSASGMQGSPFKVYKHIGIYSYRRDILLRLTGMKPSVLEEIEKLEQLRALE
                     NGLKIKVKGTSWETMGIDTPLDLERVERWLSLSS"
     gene            22582..23277
                     /locus_tag="VMU21_04900"
     CDS             22582..23277
                     /locus_tag="VMU21_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUO76898.1"
                     /translation="MDFESLISRLLLAALPILVAITFHEAAHGFVANKFGDPTAKMMG
                     RLTLNPLAHIDPFGTIIIPVMLFILSKGTFMFGSAKPVPVNFNNLGHPRRDSALVSAA
                     GPATNVIIAFLSVLLLILSYKLFPQTAPQELLSERVIGPVQAMLQYSIVFNIFIAAFN
                     LIPIPPLDGGRIAVSLLPLRHAYQFSRLEPYGIFIVIILWVTGIAQIIIRPLQLLIEF
                     ILQVFLIPFGGFM"
     gene            23274..24263
                     /gene="trpS"
                     /locus_tag="VMU21_04905"
     CDS             23274..24263
                     /gene="trpS"
                     /locus_tag="VMU21_04905"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HUO76899.1"
                     /translation="MKDRVLSGMQASGKLHLGNLVGALQNWVRLQNEYDCYYFIADWH
                     ALTTGYANPQAIRESTHDIILNFLAAGLDPEKCTIFIQSRIPEHAELHLLLSMITPLG
                     WLERVPTYKEKIQELKDRDLSTYGFLGYPLLQTADIIIYRARYVPVGVDQVPHLEISR
                     EIARRFNYLYKEVFPEPEALLTEFPKVPGVDGRKMSKSYDNAVYLSDPPDVVEQKLRT
                     MITDPARKRRTDKGDPELSPVYHLHQIFSSKEEQEHVAHGCRTAGIGCIDCKKILIKN
                     VFAVMEPIWERRNKLLHNPEIVYEILRKGTEKASRVVQETMELVREAMGLGKW"
     gene            24521..26308
                     /locus_tag="VMU21_04910"
     CDS             24521..26308
                     /locus_tag="VMU21_04910"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HUO76900.1"
                     /translation="MDTTVGNKKLNSWITEAAAMCQPDAIYVCNGSTEEYTLMMEKLI
                     ASGSATPLKKRPKSYLFRSDPSDVARVEDRTFISTSLQDEAGPTNNWIDPVVLRETMK
                     GLYKGCMKGRTMYVIPFSMGPIGSPISKIGVEITDSPYVVVNMHMMTRVSSRVLELMG
                     TDGDFIPCLHSVGAPLEKGQKDVQWPCAPIEKKYISHFPEENLIWSYGSGYGGNALLG
                     KKCLALRIASAMARREGWMAEHMLILRLTNPGGKQYHIAAAFPSACGKTNLAMMRPSL
                     KGWKCECIGDDIAWMKIGTDGRLHAINPESGFFGVAPGTSYDTNPVAMDTVRENVIFT
                     NCALTDDGDIWWEGMSDELPSHLIDWKGRDWTPASKTDAAHPNARFTAPASQCPVICK
                     DWENPEGVPIDIFIFGGRRGSVVPLVHEAFSWEHGVFLGAIAASETTSAIIGAVGNLR
                     RDPFAMKPFCGYHMGDYFQHWLTMGEKLGSKAPRIFYVNWFRKNEDGKFLWPGFSDNS
                     RVLKWMCERVDGKVGAKTSAIGLLPQEGDLDLSGLTISPENMKELMSVNTSEWKNEIP
                     DVEAHFASLGGRVPERLKTHLKGLKERLG"
     gene            26347..28062
                     /locus_tag="VMU21_04915"
     CDS             26347..28062
                     /locus_tag="VMU21_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76901.1"
                     /translation="METTTSKKTLVNVLGVVYVHTKTSDGGDLYLTRHAEPFHEHFQI
                     ENWYERDWFNANKIRLIGTSSVYKVPTKEVAGKSLELVVKNCRVGEDVPIDTHTLQEF
                     CDAEFNSPWEEFSLVTELLDNLYGPRETKMNTQLPMAIYVPPEKMQAWQSGRSRSRIN
                     RIRAKHPGIDIDILKQYKLIYEWIKGHNLMEVFAHIDINHDELVTHLKKINYKALADM
                     QRKGYLMADMKPEHIIISEADTEHIVAIGMTQERNAADKQVDIVYQCIHEGSYSIIDY
                     ELLLRTPEHDEAVKSSRRHSYLDDQRHRFDPTPLPAHLSHMEIFGVPYLYGHAESTGG
                     SLWVVGRNAHLFDYFLPERWRKTPALRLSHTKEVFYTITKDNIHLVWETSRVGEMPMG
                     DEEGSRDPKVRHYGINSPFEEFAIAHTLTQLGIPTVYARAIYMTGTTKVEASTDLRKY
                     ESHRDILDPEGNPVLQENHNYITIRGYYNGPDQWVAEHEEALYTPVDLSKAVYINIIS
                     EEQCQDFLDAVKRKLKLHGFDGTSLKVNDLLLAIDDKQQIMENSSGDPVVIICNFELI
                     WKMSE"
     gene            complement(28207..29655)
                     /locus_tag="VMU21_04920"
     CDS             complement(28207..29655)
                     /locus_tag="VMU21_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HUO76902.1"
                     /translation="MKAKYFSAVLAVLYLCFGLVSVAAPEKDSRPAAFSTGLEDQTGV
                     AVTIYNVNLGLVKDQRSLKLSKGMGELKFMDVASQIIPTSVHIKSLVNADSLQILEQN
                     YEYDLLNPQKLMDKYVGKEVKLYYKNPYTEREEIVTATLLSNNGGPIFRIGDEITFGH
                     PGRIIFPGVPESLISKPTLVWLIENSLASLQKVEASYLTNGINWRSDYVVTLNDKDDR
                     ADLSGWVTIDNRSGTTYKDAKIKLVAGDVNRAKDEMEYKKGMMRAAEAAAKPAPQFKE
                     EEFFEYHIYTLQRLATIKDNQTKQISLVNADNIGVKKELVYYGAQYYYRSSYGEKISN
                     QKVGVFAEIANTKDHNLGIPLPKGTVRVYKKDNEGSLQFVGEDSIDHTPKDEKIRIKL
                     GDAFDVVGARKQTDWKKIASDTYEAGFEISLRNHKKENVVVKVIEPIPGDWVMLSSSH
                     EHKKTEASTAEFNVPVEKDKEAKLTYRVRMRF"
     gene            30175..32853
                     /locus_tag="VMU21_04925"
     CDS             30175..32853
                     /locus_tag="VMU21_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HUO76903.1"
                     /translation="MSIKNLDVFFNPHRIAVIEASDDKRSLGYYAFRNLVGKGFNGVV
                     YPVNQRREAVQGVEAHKTILAIPHQIDLAIVASPAAELYTALDECGQKDVKGVIILAP
                     HFESPGVNAEELENQIRRLSNIYGFRVMGPNSFGFLRPSKNLNASLFPHIPRKGNIAF
                     ISQSGIFSSVFLERAISKQMGFSHFISVGEKMDIDFPDLIDYLGIDPETRAIILYVQN
                     IRSGRRFMTAVRSFASSKPIVVVRSGRAEVSSRIFLTHSGALAGEDKVYEAAFRRAGA
                     VRVYEMLDLFYMTETLGKQQRPRGKRLAIIANCQGPASMAVDALIPLEGELAALGKEV
                     MDEIEEHQTLRKRINNPVYLLPDVSPEDFELAVRSCLKDSGVDGILVIYIPFPCFDPR
                     DIAAAVVKAAKSAPHIPLFTAWLGDEQVLRAREFLSSNEIPTFVTPEQAVKSFLYMYR
                     YDFNLKLLHETPEVILKDFVPNIDKVGEIIRNAAEGGRRILRLDEAKEIFETYGIPMV
                     ETVRAVNEDEAVRLSEKIGYPVVLKIDSERLFHKLEKGGVVLNLGHEKAVREAYRSLK
                     IIASQVGDTEADVIIQPMVIKKGYELVIGTKKDPSFGAVIVFGTGGELLDAIKDYSIG
                     LPPLNQALARQMMEETKICQYLQSLPACQGILRFLEEILVRFSQLIIDFHHIREIEIN
                     PFLLSDEYGIALDGGILLEEEALTNHRRVTGDLCPPHLSICPYPFKYVMERTLRGGTS
                     CHVRPICGEDEPQIYKFFKNLADETIIYRFGHQLTHIPHETLVRYCQVDYDRELAFVA
                     ESGDGKDIIGDVRISKLPDLENAEVSILIADEWQGKGVGSMLMDYCIDIARQTGLTTL
                     WMEILKNNSRMLALSRKYDFKQAYDDEDMVRVVLKL"
     gene            complement(33392..34951)
                     /locus_tag="VMU21_04930"
     CDS             complement(33392..34951)
                     /locus_tag="VMU21_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUO76904.1"
                     /translation="MNWLISFILFLIVPSKAAAFVSHEYPAIYTHELGRIFFLIACIF
                     VLYAIVRNRLHREQGWRYILAAVITYMVWDLDVALSHFAELWIVGDTDGWNYLKQFVV
                     VEGKYYLLYVGRCDYVLLDLGMFFFYLGLRKFLEKEEESKTPFSVTALLPFLPIIISD
                     VAGALVMIALSLSCLNVSLKLYRRNRENALWYYTVWVSSSYVMYSISRAVGHIAKPLL
                     VSTGNLHIWRYVEPVSGSFNNLTFFLVGSVSLFFIVIYNLYLKMSRDEQEIEKINAEL
                     TEMNQELETLVAERTMAMMGLTVADQVRNPAMIIGCTCKRVLAKGELTPEMEDKLKEI
                     IEECRRLENVVENFECLLRSRQSIFKHEDINEVIRSVVSLVEKEASQKRLRLNINLSP
                     QPLKMNLEKNLLRAALFHIMRNAIDATSAGGEISVDSTGDGDKNSVIVSDTGSGIPKE
                     IREKVFDPFFTTKLHKFGMGLPLVKQIVSEHLGEMVLESEEGKGTTVTIMFPVRWKEK
                     QELSKYPLPSS"
     gene            35055..35681
                     /locus_tag="VMU21_04935"
     CDS             35055..35681
                     /locus_tag="VMU21_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HUO76905.1"
                     /translation="MQTLNIGRKLVLGFGAILVVMAMLVMAALMNMSALHEDLVRIVL
                     VNNKRVTIATGMATIVREEAIAVRNCFLQRERTEEMIKRINDYNAKFDEAFTQVEQMT
                     SSDDAKERELLVLVKESWTASRALNERTLALLTAGRQHEAFALYEKESRAAVRQAIQA
                     TEDLVKHQQTRSEMRYDAATRHYRDTQLFMISIGIATFLLVIIIAIRF"
     gene            36260..36595
                     /locus_tag="VMU21_04940"
     CDS             36260..36595
                     /locus_tag="VMU21_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HUO76906.1"
                     /translation="MPTMDFRGKQIEIDGEGYIANPDEWSKELAEHIAKIEGIEMTFD
                     HWDVVNFLRDYYRKYQIAPMIKMLVKEIGKVKGSEKGNTRYLYQLYPGGPAKQACKIA
                     GLPTPTGCV"
     gene            36626..36970
                     /locus_tag="VMU21_04945"
     CDS             36626..36970
                     /locus_tag="VMU21_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76907.1"
                     /translation="MRFAWFCIILFGIFIIGGLATPKPDLPFIIMGFGGLVIIAYAVA
                     KNRYWGKPIDFKELPVGEIFYVEKILRDGYVIISQPKKLPLMITGFPTELMKEGRSFK
                     KTKEMSYEVIAW"
     gene            complement(37199..38197)
                     /locus_tag="VMU21_04950"
     CDS             complement(37199..38197)
                     /locus_tag="VMU21_04950"
                     /inference="COORDINATES: protein motif:HMM:NF021550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HUO76908.1"
                     /translation="MECRNFQEKLSEYIDDILSPQEKVTMDEHLKSCGECATALADIK
                     KTIKHAKNLEAVGPPAWMTQRIMARVRAETKPRKGLFQKLFYPLHIKLPVGAIATIAI
                     ALITLYVFRTIEPEIKLAKAPTEEATPQVSPREQLPPLKYEEDKGATPPGLPLDKRGS
                     VEGLSSKPAEQPVPSKKPEPVREVPKAPEPMKQAELSQEQRRAAAPASGKDVSPSSVG
                     TLAKEEAKREAAPAAPTMKAMAEERKESIRVTVHVKDVESAQKEIERHISELGGKVVK
                     KESYENKDILTAEIDSGKLKELYERLKSVGQVIEKDIDFQGAKGEIGIRIEILSIP"
     gene            complement(38198..38806)
                     /locus_tag="VMU21_04955"
     CDS             complement(38198..38806)
                     /locus_tag="VMU21_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUO76909.1"
                     /translation="MDRNEYAGMDEDDEAVSRCKQGDLDAYEGLVEKYQKKMLNVAYR
                     IVGSYEDACEIVQDAFVSAYKGIREFEGKSRFSTWLYAIVVNLSRNRLKQLRTLGHHE
                     GLSMDDPVLTDEGSLSVEVASDDPPVDERLEQEELQRKVRDCVRALDEEYREVVVLRD
                     INGFSYSEIRDMLRIPEGTVKSRLSRAREALKDCLKKVLGEL"
     gene            complement(39046..41538)
                     /gene="phnD"
                     /locus_tag="VMU21_04960"
     CDS             complement(39046..41538)
                     /gene="phnD"
                     /locus_tag="VMU21_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HUO76910.1"
                     /translation="MKNIFGDLKISGKLLLTPIVIILFMMIPSVAAFIGLYQQKGGID
                     SVLYLLVALFGVSVFLSLLVSFLILNKRIISPLKAMETAARKISEGDLSFDVEVSDKD
                     EIGRLSFHLRESFEELGGILQRIKELSERISRVVEEVERESKNVLNGAEKETEAITDI
                     STSIEELNATATEIADNTESLAASTGETSVSVEQMVQSISSINGNIQGLSREVESTSS
                     SIEELSSTIKDVASNAVDLATAAEATLSSISEITFGIREVEDNIKESARLSEKVTSDA
                     ATFGMASIEKTIQGMKNIQSSVEHTAQFIEVLGKRSDEIGKILNVIDDITDQTTLLAL
                     NAAILAAQAGEHGKGFSVVADEIKDLAERTSLSTKEIVSLIESVQEGVTNASGAMEKG
                     IMSVQEGFMLAQEAGDALKKILDSSRISSEMALSIERSTVEQAKAARLVTEAMEGVRD
                     MTGHIAKATAEQSKSALMITNAAEKMRDASHQVNKATGEQAESSKQIIAAIELVADRS
                     RQISRSLSEHKKGSKSILSSVEGVKDIPMENRRLIFRVSNTLRDLQKDSELLRTEMER
                     FRFHEKKGAALKFGVIPLESPAEMFRRFGPLVNYLGKRMGKRVELKVGVDFEGAINDI
                     GQNVTQLCFMGAPTYLKANSRYGSVKVIARALRKGRPYHHAAIITRADSDIASLKDMR
                     GRTFAFANIISTAGHIIPRTMLKDAGVGIDDLKYYSFLGNHDDAARAVLKGDFDAGSV
                     MEATAYKFIDQGLRILQRSDDIPEFGVCCNISLDGKEVRTIQQALVAINDSTPEGAAI
                     VKSIDKNYTGFIEADDSEYSGIKLKMAEFGIL"
     gene            complement(41785..42438)
                     /locus_tag="VMU21_04965"
     CDS             complement(41785..42438)
                     /locus_tag="VMU21_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76911.1"
                     /translation="MTSKNRDKIRTIYRTLYKAFGPQHWWPGDSPFEIAVGAILTQNT
                     NWGNVEKAIHNLKAAHVLSAKRIHEMPVNTLAAFIRSAGYFNIKAKRLKSFVHFFCEQ
                     YGGSMKSMGKEDARILREQLLSVHGIGPETADSILLYALDKPIFVIDAYTKRILSRHS
                     MIGHDEPYSDFQELFHLSLKRDVRLYNEYHALFVATGKFFCKPKPFCAECPLLLLEH"
     gene            42511..43800
                     /gene="serS"
                     /locus_tag="VMU21_04970"
     CDS             42511..43800
                     /gene="serS"
                     /locus_tag="VMU21_04970"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HUO76912.1"
                     /translation="MLDIKCVRDNPEKLREALEKRGIGDFFVSEVQLSLGQLVEMEDK
                     RRRLLKEVEELRNKRNVVSEEIGRLKREKKDADSHLTEMKGVSDKIKELDETLGKIEE
                     RETTFLHYIPNIPHASVPVGKDETENTVVRIWGEPKDLGFPPLNHWDIAEMLGIIDFD
                     RASKIAGARFALMRGAGARLERALMNFMLDLNTSKGYTEVFPPILVNRESMTATGQLP
                     KFEMELFRIADPEFYLIPTAEVPVTNIHRDEILKEDNLPLFYTAYTPCFRREAGSYGK
                     DTRGLIRQHQFNKVELVKFVTPESSYEELEKLTSDAEDILQRLCLPYRVVALCTGDLG
                     FSASKTYDLEVWLPGQQRYREISSCSNFEDFQARRANIRFKREGKKGTEFVHTLNGSG
                     LAIGRTLVAILENYQQKDGNIIVPHVLRPYMGMDIIK"
     gene            43917..44462
                     /locus_tag="VMU21_04975"
     CDS             43917..44462
                     /locus_tag="VMU21_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76913.1"
                     /translation="MKKHMAKILVSVIMLFGFAAQGLAEPPDFEKPPTREQKERVRQR
                     IETLRMWKLTKDLDLDEKTSAQLFPLLNRYDKKRAEIEHAMQESMKELGESLREKREG
                     QLRGILDRLEQNHKGMQSLKDAEWEEMKKILTIEQQAKYVIFLQEFDREMRRIIEEAR
                     GRRHEGLRKDLPEKQPAQERK"
BASE COUNT        11061 a        11691 c        10289 g        11514 t
ORIGIN      
        1 taaggctaag gttcagacaa aacaataccc tacattcctc aacctatatt ttttcttact
       61 aactatttac cgaaaaatga gttccatctg ctccaggtgt tcccccggga aactgatggg
      121 atattcatta tcgaaacatg ctctgcagaa atctcctgaa tcaggcacta tctttttgag
      181 gccttcgagg gtcaggtacg caagggaatc agcggtgata tatcttcttg tctcttctat
      241 ctgatgggta taggcgatga gctcctgccg cgtgggcgta tcgataccgt agaaacaggg
      301 tccgatagtg ggtggtgaac ttatcctcat atggacctcc ttcgcgcctc ccacctccct
      361 gatcatctta atgatcttct tgcttgtggt gcccctcaca atggaatcat ccaccaccac
      421 caccctcttc ccttctaaaa gttgtttcac aggattgagc tttatcttca ccccgaaatg
      481 gcgtatgctc tgtttcggct cgatgaaggt ccggcccacg tagtggtttc gtataaggcc
      541 gaaatcaaaa tggatgccgc tttcctcaga gtatcctatg gcggccggaa ccccggaatc
      601 aggaacaggg atcacaatgt cggcatccac aggaagctcc ctcgcaagct gccgcccgaa
      661 ttccttcctc acctcattca cgttctgata cccgaagatg taactgtccg ggcgggagaa
      721 ataaatgaat tcaaatacgc agtgcgcctt tctggtgccc gggagcgccc tcacagacct
      781 cagaccattc tcatctatga tgagcatttc tcccggctcc acatccctta tatatgaggc
      841 attaatcagg tccagggcac aggtctccga ggcaaccacg tatgcgccat ccacctgtcc
      901 gagacagaga ggccttacac cgtactggtc cctcacggca atgagctcct tttccctgag
      961 gatgaggaga ctgaaagcgc cgctcaccct gctcagtgcc tgaataatcc ggtcgtaggg
     1021 gttgtctccc ttggcatggg cgatgaggtg gacgatcacc tcgctgtctg atgttgactg
     1081 aaagattgct ccctcatcct ccagctcttt cctcagttcc gcggcgttca cgagattgcc
     1141 gttatgcgca agggcaaggg ggccgagggc aaaattcgcc atgatgggct gcacattctt
     1201 cagtacgctg cttcctgcag tggaataacg gttgtgtcca atggccagat agccggggag
     1261 tctcttaagt ctcttctcgc taaagatatc cgcaacaagg cccatggact tttcgagata
     1321 gagttgcttg ccgtcagaag aacagatgcc cgctccttcc tgtccccggt gctggagggc
     1381 gtaaaggccg aggtaggtga ggttagaggc ctcgggatgg ttaaacacgc cgaatacccc
     1441 gcattcctca tggatatcgt ggaacatatg ccgtaacaaa gcgttcacct gcacatccct
     1501 cacagtctcc taggatttct cggatggaaa acgaatgtac ttctcccagt atgccaaaag
     1561 ttcccttgtg gccctgagat cgccaaaaca gtatttcgcg atttcgagat atttgcccga
     1621 tgaatagaga tccttcacct catatccggt gatgccctcg gatttggggc tcttgatacc
     1681 aaaggtcctg caccacatgt ccaggctgaa ctttcttctg gatgcgccgt aaaaggtgag
     1741 ctggtcaaag agatcaatat gcggaccact atacctattc ggcatgaggt ccctggacgg
     1801 ctttatcttg tgcactgcgg accgtatcat gataaaggga cagtcaaagg acctcccgtt
     1861 aaaggtaatg atctggtcat acccttttat gagctcccag aatttctgga tgatccccgg
     1921 ctcagtgccg gtctcgaagt gtattgcgtc ctcttcaaag ggtaaaaggg gattttgagg
     1981 tgcctgaaaa tataccgcgc ccttcatggt atcgggattg agcatcccga tggccacgat
     2041 ctcacccgtg aggggataaa aggagaggct gtccttcacc tcctgaatat cctcctctgt
     2101 ctctgcccac ttgaggagat agtcctgtgt ggtcttgtca aaagacccga aatccctccc
     2161 cacggtctct atatcaacga taatccttga catttcctca tttgccagca ggcagtagtc
     2221 aggaaaatat cctcaattca ctacttacga cttactcctc tcgatttcct gtttcctgtt
     2281 cgttcttatg atgccttagg cagctccttg aataccttcc atgtcttaag caggtctatc
     2341 gccctctgca actggacatc atccttttca gatacctcca ggggagccat ctcctgctca
     2401 gtcttgcttt tttcttcctc tttctcattc ttgagatgtc tctccagatc tttttccctc
     2461 accacggtat gttcctttat cccattcttt gcttcgatct tcacgacaat atcgggcgtg
     2521 ataccggttg actgtatgga agtgccactg ggcgtgtagt agcgcgctgt ggtcagcctg
     2581 agaccggaac catcgctaag gggtatcacg ctctggacag aacccttgcc aaaggtctgg
     2641 gctcccagta tcacggccct gcgccaatct ttgagtgccc ctgccacgat ctccgacgca
     2701 ctggcactgc cctgattcac gagcaccacc atactcggtt tgtcataata ggtataattc
     2761 ccccctgtac ggtattcgtt cttctctcct gccctgtctt ttatatacac cacgagcttc
     2821 cctgggggaa ggaactggct tgtcacatca accgcactat tgagaagccc gccgggatcg
     2881 ttcctgagat cgagaacaag ggaattgaca ttttctgagg tgagctgtga aagggctttt
     2941 gaaaggtcag aagcggtctg ttcctgaaac tgggtaagct taatataccc tatcccttcc
     3001 tccagtacct tgtactttac gctctttatc ttgataattt cccttatgag agtgaactcc
     3061 ttgggctcat tccatccctc tctcataatg gtaatcttca ctgatgtccc cttgggtccc
     3121 ctcatcttgt tcactgcatc cagcagtccc atatcccttg tgatttcgtc attaatcttg
     3181 actatcctgt cacccgcctt tatccctgcc ttgtatgcag gggtgtcctc aatgggagct
     3241 attacggtga ggacattgtc cttaatgcct atctgtatgc cgagaccgcc aaactcgccc
     3301 ttggtgtcga cctgcatctc cttgtacgcc tcagggttca tgaagcccga atggggatcg
     3361 agggcattga ccattccctt gattgcgcca tacacaaggt ccttggtttt tgtttcctcc
     3421 acgtaatttt ttttcactat ggaaagcgcc tcggtgaata tcttgatgct ctcgtatcct
     3481 tcaacctcgg cgtttacaag ccccactgac caccttccca ccgcaatacc cgatacggca
     3541 atggtaaaga ccaaagccaa ggtaatcagc atcttccttt tcgtaatcat ccttctttat
     3601 cctccgtcta ttttctcttc agccactgca tgggatcgag gggtttccct ttatagcgca
     3661 cctcaaaata cagagaaggg gcattcacaa ttcctgattc ccccactttg cccactgcat
     3721 ccttaatctt tattatatct ccaactttca aaaaagtctc tgagagattt ccatagagtg
     3781 tgtgataccc ctccccatgg ttaatgatca caagattgcc atacccctta aaccagtccg
     3841 caaagaccac cttgccggca tacactgcct tcactgaatc ctcatcactc ttgatataga
     3901 taccgtttct gaagaccggg gtgttgaatt tgggatccct ctggctcccg tagggaatgg
     3961 ccacttttcc gttcacaggc cagatgaggt tccccctcag ccctgaaaag ccctttgcct
     4021 cataggtatc cttttcttca agcttcctca gcacatcaag gagccgtttg gaagcctcct
     4081 gaagctcctt gagcaatttt tcttgtgatg ccttttcctt cctcaccgat gcgaggatga
     4141 tttccttgtc cttttttgtt tccgagagcg acgcctctat gggcttgagc ctttcctgac
     4201 ctttcttcag ctctgcacgg agaccctgca gctgtttttc ctttctgtcc agtgacctga
     4261 ggttctccgc aaattgctca atgatcttcc gatccttcat ggcaatgcgt tcgagatact
     4321 tccaccttct tacaagggct gccaggtcat ccgttgcagc gagaagaaag aggatatccc
     4381 ccgattgccc ataccgctgc atggcctgga gtcttctttt gagccatgcc ctctctttct
     4441 ccagttctcc ctcaatcacg gatatctcgt gctcaaccct tccgatctct gactcggtct
     4501 cccttatgcg ctgctgctgc ttttttaact ccgcctcaat ggtactgaga ctcctgctca
     4561 catggtcaag gtcttcaagg accgaacgct cacgcttttt tgcgtgctcg agtctctgtt
     4621 ttcgggtctc catttctttt tggagtttct ggtattgttc tttcgcatct tcagcagaaa
     4681 caatgtccgg gaagagaagg cctgaaaaga aggaccaggg aaaaaagaac aggagaaatg
     4741 cagcatacca catcaggtgc ttgaagtaac ggactcctac aatgttgctc tgcgcattgt
     4801 gcattgttac cttactaaaa tttaatcctc cccatggcga taaaggaacc ggtaatcccg
     4861 aggaacagac caatgagggg aagaggcagg aacagctctg cggggaatac cacagtcctc
     4921 actatgggca gggtagcact caattggcgg aacacactaa ggtaaaatgt gataacgaca
     4981 atcatactga gcactccccc cacgaccccg atgacggccc cttctatcac aaacggggtt
     5041 ctgataaacg ccctggttgc tccaagcagt ttgagtgtct cgatctcttc ctttctacgg
     5101 taaaagagta tcttcaccgt gctataacag atgaaaatga tcccggaagt cgttaccaaa
     5161 gtaagaatga gaccgatggt ttccatcgcg gtcttaaggg aatgaatgga gaggagaaat
     5221 ttctccccat actgcacatc ctcgataccc tctatctttt tcatggaatt cacgaacgtc
     5281 tttaccgact cgggtcccat cgcttctttc ttgaaccgta tttctatgga tgcgggtaat
     5341 gggttctcat tcaacccctc gaggacatat tcggcatcct taagggatgt tcttagctcc
     5401 ttcaatgctt cggcctttga tatgtatttc accttctcta ttccgttccg ttccttgagg
     5461 gaggcaatga tgtcctgggc ctgctgctca tttacccggt caccgagata ggccacaatg
     5521 aaaaaccgct cgggaagttt tcttgcaaag aggttgatat tatagatggt cgataccata
     5581 agggtgatca ccagtaatcc catggcgata gtgagcatgg aaaggaagtt gatccacttt
     5641 tcgtgccaca ggctctggag cgcaattctc agtgaatagg gcattggcta ccccttctcc
     5701 tcccgcacca gggaggcgtt atcaagcctg aggatcctca aacccgtgtc tttgaacaga
     5761 ttgggattgt gggttgcgat aaggatcgtg gtgccctttg cgttgatctc cctgaatatc
     5821 ctcatgattc ccgcggcagt gtcgggatcg aggttccctg tgggttcatc agcaagcaga
     5881 atagtggggt tgcccacaat ggccctcgca atgactactc tctgctgctc tccccctgaa
     5941 agggcagaag gaagattgtc cgccttatgc cggagattca ccatcttgag ggcatcaagc
     6001 acctgggctt tcacctctct ctcggggacc cgccttaccc tgagggtcaa cgcgatatta
     6061 tcaaagacat ttctattaag caaaagcctg aagtcctgaa agacgatccc gatggtcctc
     6121 ctcaacttcg gaattgtcga accgttcagc ctcgcagtat cccatgtcgc aactgatatt
     6181 ctgccgctat caggcatctc tgcagcataa atgagtttga gaagggtgct cttgcctgca
     6241 ccgctcgggc cggtaaggaa gagcatctcg cctttctcga tgagaagatt aatgtttctg
     6301 agggcagtga ccgtatcgta aaattttgat acctcctgaa actctatcat aaggatgtct
     6361 tgatcgtgtt cacaatagcg tccggggtga gcccgtagaa ctccagcaat tcctcaggtt
     6421 tgcccgaacg gccaaaggtg tcccccaccc caatgcgcct cactttcacc gggtgttttt
     6481 ctgaaaggat ctcactcacc gcgcttccca gccctcctat taccgaatgt tcctccacag
     6541 taacgataag cccggatgat tctgccgccc tgagtaccgc ctctgcatcg aggggtttaa
     6601 ttgtggagat attgatgacc cccacatcaa gcccctctga tttcagtatc tgtcgtgcct
     6661 caagggatgc ggagaccatg atccctgtgg caatgatagt tgcgtccttt cccaggtgaa
     6721 atatatgtgc cttgcccagc tgaaaccggt aactctcagg cgtcaccagg ggcaccttca
     6781 tcctccccag cctcacatat acaggccctt catagtcggc gatggaatag atcacctgct
     6841 cggtttccct tccgtccgca ggtacgatta cagtcatatt gggcagtacc ctcatgaggg
     6901 caatatccat cagtgcctga tgggatgccc catcctcacc caccgtaatc cccgcatgtg
     6961 tggcaacaag ctttacattg agatttgagt agcagatggt ctgtcgtatc tgttcccatg
     7021 cccttcctgt ctcaaatacg gcaaacgtgg acgcaaaggg aagtttcccg gccagggaga
     7081 atcccccggc agttcctatc atatcctgct ctgatattcc cagattgaag aatcgctcag
     7141 gaaaggcttt ggcaaacttc gcggtctttg tggagcccga aagatctgca tccagtacca
     7201 ccacatcgcc cctctttttc ccgagctcca gaagggcagc gccataagca tcccgcgtag
     7261 ccaatgcctg ggacgttcct cccggtgagg gagaaccttt tctctcgccc attatttcag
     7321 ctcctccaat gctctgtccc gctcttccct tgtgggtgca ataccatgat actctgcctt
     7381 gccctcgaag aaggacaccc ccttcccctt caccgttctt gccacaatca cgctgggtct
     7441 gccctttacc ctttctgcct catccagggc atcaacaatc tgctccatcg catgtccatc
     7501 aatgtccatg acatgccatc caaaggcctt ccatttctcg gtgatgggct cgatcttcat
     7561 cacatcgcag caaaaaccgt ctatctgtaa accgttgttg tccactatgg cacaaagatt
     7621 gtcaatcccg taatggacag cagtcatggc agcctcccat acctggccct cctggagctc
     7681 accgtcaccc aaaagacaat atacccttga cccgaggccg tccatcctca ggcccatggc
     7741 catcccattg gcaatggata acccttgtcc aagggagcct gtggatatct ccaccccttt
     7801 gagcatcttc gaactgggat gtccctggag cggactgtca atcctccgga gcgtcttgag
     7861 aagttccctg tcaaaatatc cgctaagggc gagggccgca tagagaaccg gagctgcatg
     7921 gcctttggag agaacaaacc tgtccctctc tctccattca ggctcctccg gcctgtgcct
     7981 catcttataa aagtagagcg ctgccacaat atcagcagca gaaagggagc cgcccgtgtg
     8041 gcctgatccc gcctcggtga gcatcttcac gatctctctc ctcacctcct ttgcgcgctc
     8101 gcgcaaaaga ccgacatccc ttgtagctgt gatccggctc acccgttctt taccttcttt
     8161 gaaatatgtt caaggagaat ctcatcaagg ctcttgggag ccttattctt gctctcgctc
     8221 ttctccggca tcaccgcact cttctcccca ggctggggaa tgggttcctg cttatctccg
     8281 ggcagcttcg cctcgccctt ccctgtgagc agttccttta tcgcttccct gtgctggagc
     8341 ttcataaggt tcttgagctg tttttcgaaa tcaggactcc cgataatatg ggcataactt
     8401 gtcttgaccg agcgaaggat cgcaccctgg tgatagagga gtgtcacaat ggaatggctc
     8461 ttcagaccgc tgtcctctgt ctggacatgg tattgcctcc ccttatggga aatattcgta
     8521 ttgtaaccga taagcatcaa aactctcgct gctcagggtt atgcaacgca ctgaaccatt
     8581 acgagagata ttatatactt cattccccgg gagatacaag tgagaggaga ataaggccgg
     8641 aaagcgtgaa aaactccgct acctctttct ttcgtaccgt tccatcacat agaacatgcc
     8701 ccctgccatg agatagacaa aggccatcac cacccagtgg ctgatgccca ggaccgaggg
     8761 aatggtagct ccttccatgg gtgacgagaa gtagaaccct tcaatcgcgg gatacacaac
     8821 cgcaaacacc agagatcctg cccctatccc gcccatggtc accagggcgt ccaacctccc
     8881 tgtggcaaaa ccagctaccg ctgttcctgg gcagtacccg ctgaacagga atcctatgcc
     8941 gaagagcgcc cctcccacca gctggggcca gaagaaggcg gggacaatcc atatcctgtt
     9001 catgtcaaga accttgaggt ccgaaaggag ataaagccct gtagcggcaa ccagcattcc
     9061 ggtaaacatg accttgggga cactgaagtc agtaaggtaa aaaacccctg taagcttgtg
     9121 ggggtcaccc agacctcccc tctccagaaa aagaccgaag aaaaaaccga ggatcaccgc
     9181 cagcacaagg cctgcggtat ggcttaccat aaagggtgcg ctcatgacca taacctcctg
     9241 aacaatgccg cggcagcaaa gccgcccaca aacattgcga ttacaaaaac ccagccagct
     9301 attgcgagct gagcccctcc tgacagtgcc accccgctgg tgcatcccct tgcaagcctg
     9361 ctcgcaaagc ctataaggat accccctcca aacgcgctca tcagccttgt tccagtactc
     9421 atatgctcag ccttatcgaa tctcaccctg aagtttccgc ctgcaacgga tcccacaagg
     9481 gcacccacga aaagtcccat gatctcaaaa agcacccagt tcagtagcgg tgcttcaaca
     9541 ttcagatatt gagaaaaata ggttattttg tgggcgtatg ccggactgag tttgtccaga
     9601 ccgacccctg ttaggagaga aaaggcactg gaagcgccca gaccccatcc cacgataaca
     9661 aaggttgcga ggagcgttaa gccgagacct gcccctgcta tataaggtga ccagaaatta
     9721 tcatttttca taccaacctc catagagtcc actgacccct cttatgctgt atctcttctt
     9781 tgctaccacc ttagtatcct cacacagcct cagagttgct ttgttgctaa acttactaac
     9841 cattttacaa ttatatcact gaaaaatcga caacacaaca ccaagataat atcaggagag
     9901 gggatgatag gaaaaggaca agttatggct ttcgggaagg gagacaagtg agaggggaca
     9961 ttgtccccct ctcatcctta gaatctccag gtgaatccga attcgaggct gtcctcatag
    10021 agactagatt taagggtcac cggctgtccc gcaaaattcg taccgctttc actgatgtcc
    10081 tctttgaagg catgggtgta cccggcattc accgcaaacc gctttgagat ttcatatccg
    10141 gctccaaagg tgatatgctg cttgacgatg gcagggaatc ctattattct gaaggtttca
    10201 taatagtagg tcggcatgtt atttccctga acggaaatca tgttaggacc tgctggggtg
    10261 aggcccacga aattgctatg ttccctcaca gggttgttcg catagttata tccgatgcgc
    10321 agggcaagtg gcttgatggg tttgtactgt gcgccgattc caaacaccca ctggtccttc
    10381 cagtcgaaat cgtcatagcc cttggcgttt gcccagttta tccactttcc attgacctcc
    10441 accagcagcg tgtccttaat aggctcaaca gctattccaa gccctatctg ctgaggggat
    10501 gcaagcttca ggctccccac agtaccgcca agattaagga cattcttata attgacctcc
    10561 tgtggtgtca cataggtaag gccgagggag agcatatcca agggcttgta caatgccccc
    10621 agttggaccc caacaccgta attaaaggaa gtgccgcttc ccaggtcgag gctagaatag
    10681 tcgatttgaa ggttaaggcc gaaagagagg tttggattga ccgcataggc aatagacggg
    10741 gcaaatttca ttgcctggag ctgggtatat gagcccgcaa taaggggagt tcccgcagga
    10801 aatggcggtg cgggaaatgt gccggcgaga cccgtgttcc tgtagtcaac gccaaggcct
    10861 gatacgccgt aggctgaaag tccgaatctc cagtcagtcg tgatcgggac tgacagaccg
    10921 atggcaggta tcgcataaac cttcttgtca ctgtccgcgt ttacggtctg gccggaggtc
    10981 gtcaccgaac cggtcacatt gggcatgaac agagttcccg caaaattcac ttcgctcccc
    11041 ggacagtaag ggccaaaaca catggcagca ggattggcaa agactgcgct gattgcatcc
    11101 tgaggagcgg cgatacccac ccctcccatg cccctcgaaa tgggaccgat accgataaga
    11161 ttgtcaccgt ttgtcgccat ggccggaggc gtcacgactg cgaccatcac taataccaga
    11221 agcaacaaaa acaattttcg cattactccc tccttttcat ccttgatttc ttgccgctta
    11281 ctccctcagc ttccttcttc tcgatcgatt ctccccccac ctcctctccg cgaatagcca
    11341 ccctatttga gattggcatt caccatatca acgatttcat cctgaatctg accaggcatt
    11401 gctccttttg actatttcac tttcttaatc agaaagctga agacattatc tttttcatcc
    11461 atctcaacaa gttcatttcc cgtccttcta agcatggcgg ggatgtctgc cttcgaccct
    11521 ttgtccgtca cctcaacaag aaggacctgc cctggctgca gggaatccag cgccttcttt
    11581 gtctttagca caggcatcgg acacgcgagc cctttcaggt cgacggctaa gttcgctttt
    11641 gacttatcca cagtatctca cctctctttt cctccttaat ttttcctgag tttcggcgaa
    11701 tcaaataaac aggcttatat ttgcatcaag tgcaaaatcc aggaactcag cagcccccgc
    11761 cacctcaacc ccctcaacga ggtcctcctt ggacagccct gtcatctcca gggttggagt
    11821 acacgcgatc atcttcaccc cggactctaa acagacatgc aggagctcgg ggatcgtagg
    11881 ccagttgatt ttcttgatca tgcccttcat catcatcgtg gccatgccgg tcattccagg
    11941 cagcacgcca aggatgttcg ggaatggaac aggggaaggc attgcagggt ttgcgatggg
    12001 ggcaacctga agagaatggt attttttctt gttcacgatg tccacaccat agagggtaaa
    12061 aaatatctcc acctccacat ccatggccac tgcagtagat gcaaggatta acgggggata
    12121 tgccatgtca agagttcctt ttgatgcaat aagtgccatt cgctttttct tctcttccat
    12181 attttcacct ccgatagtta cacgttatcg taaagtatag tgaagtcaga ggggcttgtc
    12241 aacaatattc gaataaacta aactataggc ataaaaatac tttgactgag gtttcttgag
    12301 agatttctca tgaattttcc tgtagattcc tatatgcgag gctgcaggag ggggtccttc
    12361 atccccgcct ctgcaaaacc ttttgctcta aagatgcagc tgtcgcattt ttcacaggga
    12421 acatagatag tgcagaagtt tgaaagtctg aaatcttcac tttgagtttt tcttggctgc
    12481 ggatcatagc agctccatgt gagagaataa tccagcccca gatcctttcc ttttcttatt
    12541 atctcctttt ttgtcagaaa aaggagcggg gcttttatcc tgaacctgag tttcccttca
    12601 acggatgcct tagtggcaag atttgccata tcttcaaagg ctttcagata agcaggacgg
    12661 cagtccggat agccgctgta gtcaaccgta ttcgcaccaa taactatgtc ctctgcctcc
    12721 aaaacctctg cccatgccaa gccaaaggaa agaaagatgg tgttacgtgc aggaacatag
    12781 gttacaggga ccggggatgg gatatctgct ttcttgccaa tccctgactt cggtacttcc
    12841 atctccgatg tgagggcaga cccgcctatt tccctgaggt caacctttat gatgagatgc
    12901 cttgcgacac ctaaactttg tgccaccctc ttcgctgact ccaactccct gttatgcctc
    12961 tgaccatagt cgaaactgag agcgtagacg tcgtgaccct ctgccaggaa aatcgcaagc
    13021 gcggttgacg agtcaatacc cccgcttaac aaggctaccg ctttattacc cattttcttc
    13081 caaacaaaag ctcagcctcc aaaaaaagtc cacgttttga gaggaaagcc tgaggttaag
    13141 catcaataca ggtcatctgc agtacccgct ttcccgtcag gacctgtgct caaaatcatc
    13201 acatttccgc tctcgacgct atatataaag ggtcttcccc aggaatccct ggcatcgccg
    13261 atctgattta tctgcgaagg gtacgaacca ttcctgtatc tatagacctc tgccatgaat
    13321 ctcagctctt cgatagtact cacagttctt agcctggcaa atgaatcacc attccaggaa
    13381 agggaaaacc ccctctgagt tcccgtcccc tggaagaccg ctgcaatcaa tgagatgata
    13441 agggtgaggc ataatgcggc agggacaaag aaccttgcca atggtctttc ccggtggaca
    13501 accgggatgg ccacccgttc tgctttcaca ggagcggctt tcaccggttc aataatttcc
    13561 ctttccagga gagaaaggaa tatcctggat gcctgaaaat cttccatacc gcagagatca
    13621 atgataatgc tcacatcatt ttccccatca acaaaattga gtatggtttc ttcctcggtg
    13681 gtcaatgcgg gacctgtctt tcccgtgatg gtgaacactg tttccggaag gatcttccct
    13741 tctgcgaggg accattcgtc cataatgcgt aagccttcca tgaggaggtg ctgcgtgtcc
    13801 agtgtgagcg caagatcctt gctcaccgaa acttcctggg actgaaactc gtacgtacct
    13861 tctttccaag aaaacagctg cactaccgtc tcggtcatct gcgagatgag tgtttccttg
    13921 atatcttcct tggtgaccag tccttttctg agaaacacat ctccaatcct tgcacctgtt
    13981 cttttctgtt cctcaagggt agacctaagg tcttctcctt ttataagccc cttcttcagc
    14041 agaatcttgc ccagcctgtt atcctccatc cgctttcgtg actctgccga taccacattg
    14101 ccttcggaaa agatcagttg aaccttgtcc ctgtgcccgc tcagggaaag aatgccgctc
    14161 tttttctgga aatagatcag ttgcaggatg tccgcaagac caaaatccgt cagagatcct
    14221 tctaaagcca tcccttacgt atccccctcc ctatatgcaa aatggagaac agataagtga
    14281 atgccatgag catcactgcg agcggtgtta tccaggcatg gctgaatggc cagatcccca
    14341 cggtgggaag gttgttcaat agaaacaaaa caagggagaa agaaaacggc cacaacaata
    14401 agagaccgag cagtatcttg cccgagtata tctggcctgc ccccgggagc acacaagaga
    14461 gtatttttgc tcttttcctc cgtcttgttt tgctctcgta tacggagagc aacctcgcga
    14521 ttctctcttt ggaatccacc tcatccatct ttgtgagcga agtgtaacac tggggacaca
    14581 tctctcccca cgtaataacc cttgaacacc tgctacagaa tactgcgctg cacctcttgc
    14641 acctttgcgc cctatgcccc gtccctttat tgaggagata aaagccaggg atcatcagaa
    14701 acgccatgag agtaatgagg agaggaaacc tgtaaagaga accgttcccc atccctaacg
    14761 cgtactccca gagcgcagag ggggggagca tctcgtccac tacaaaacgg ttcggaccag
    14821 tgcctgaacg ggaagtgaat ttggatacgg cctcaggatt aagcttggca gcctcgagga
    14881 aatattcgtc tcccttcgca aagtcaagca tctccctgtg aagctggctt agattgtaat
    14941 acgcagaggg gaggggcgaa cgctggaggg ccttctgata ggaatcttct gctgctgccc
    15001 tgttttgaac cgcatagtat gcattcccga ggttcacgaa gagacggggg cctgacctgc
    15061 ttgagtctcc cataagactc aagtacgact ctatcgcttc attaaagtta ccctccctct
    15121 taagcgccag ggtatgggag aatcgggagg cgaagtcttc tctcccttta atggcccata
    15181 acgcatacct gttgttcttc ccttcgttca cttccactat tgccctcagc ctaggtgagg
    15241 gcgccgaaaa gaatttacct tccacattca gaagggttgg cgcaaaaaga aagaacaaga
    15301 gggctgcata caccaccacc ttattctctt tcttgaaata acccccgata agaaaaaata
    15361 tccccgcgat gagcgcaatg ggcccgaaca gggaaaggat gacagggacg agcaggagaa
    15421 ccagtcgcct ttggtcctcc atcccgtcgt gtgaaacaag accagcgtcc atgaagatcc
    15481 tgataatcag aatcagaaca agggagaaca caagagagat gagaatgctc gaatagagca
    15541 atcccaaaag ggtaaatgcc caccaaaaat tcctcccgta agccttaatt ccttccctga
    15601 aagaatcaat tgcctggaat atatcaccca tgaaaagacg ctcactggca agtgcaaagt
    15661 acactgccgg caggtcaggg gaatactgtt ttgccatttt cagaaaatcc cttgcatttg
    15721 ccggttcttg gtgggccttt gcgatgaaaa gatacgagta cggctctgtg gtggagagtc
    15781 cgcggtccag cctcgactga tagagatcct cgactccttc acaaggcccg gctccatgaa
    15841 caaagcccca tatgccgagc agcacaatcc agaccgtaaa ggttttggac ttagaatgtg
    15901 cgactgtaaa ctttgccatt aaaccctctt tcctattttg ggttctgtcc cctgtagtaa
    15961 tctctttata ttatcagtat gccgtaagac aagcaaagac gcgatcagga ttgatacgat
    16021 cagtttttcc tgtgtatagt ctagtacata tacgcctaga ggtaaaaccc caaaagagac
    16081 tagggctcca agagaagaat agcgcgtgac cagtattaca ataagccaga gaatgacggt
    16141 aagcaccccc accttgggag aatagatcag caggacgcca atgcttgtgg caactccttt
    16201 gcctccccta aacctgagaa acaaagagaa attatgtccc actattgcgg ataatcccat
    16261 gataccttcc agggacggac tctggaaaaa gtattttccc acgacaaccg cgagggagcc
    16321 cttcaataca tctccgacca gggtgaggag tgcaggacct ttccccatcg ctcttaacac
    16381 attagtagca cctatgtttc cgctgcccac ctttttcagg ttcaccccgt atactcttgc
    16441 aaccacgacc ccgaagggga ttgaacccag gagaaacgca aagagtatca gggaaaaaac
    16501 gttcatatct ttttccagaa tgaaacagtg tactgaacac ctgacacgat cgaaagtatc
    16561 agggctatcc atatggaaag tattcccaca tcgtaaaggt cgataccaaa gatatccgta
    16621 ctgatgatta ggcagaggat tgcggtgatc tggaaggctg tcttgagttt tccccccatc
    16681 tccgcgggta tcacgatatc ctttgaaagt gctactgccc tgagccctgt gacgagaaat
    16741 tctctcacaa tgatggtgat ggcaatccat gcagaaagcc tcgccatgtc tacaaggagg
    16801 atgagggcgg atattacaag aaatttgtca gcgatggggt caaggataat cccaaacttt
    16861 gtaatttgtc cggtccttct cgcaaggtag ccgtcaagga aatcagtaat tgacgcaaca
    16921 ccgaaaatca gggcgcccag gaacggtctg tcgggtgcaa cgaggacaaa aaaagggatg
    16981 agcacaattc tgctgagggt tatgagtgtg ggaaggttaa gcctcaaaat actccttcgt
    17041 cacggattcc cagagccctt ttgccttaag gagaaaatca cagacttcgc gcaccgcacc
    17101 tctccccccg cggtgtcgtg tgaccactgc tgcgtgatcc cttgcttcgt ccgctgcatc
    17161 actcactgca atggggactc caactctctt aaaaatggga atatctacta tgtcatctcc
    17221 cacgtacgca acttctttct ctgtgaggcc gtatttttcg atgatgtgat caaatgccac
    17281 agtcttaatg tgacaccgct gatagacctc tgtgatcccc agttcacggg cccttcgctc
    17341 cacaaccttt gaatgcctcc cggtaataag ggcaacccaa atgcctgcct tgacaagcat
    17401 ctttatgcca tggccatccc tcacattgaa tatcttgaat tcgttcccct cgttgtccag
    17461 gatgatactt ccgtcggtga ggaccccatc cacatcaagg atgagaagtt tgacgtcctt
    17521 tgcgatcccc tgaagttctt tctcacccct tttcatttac acaatcccct gtttcagaat
    17581 atcatggagg tggatgacac cctttgccct tccctctccg tccggcacca caagggaggt
    17641 aatggagtag ttctccatca gagacaacgc ccttgcagcg agctcgtcct cagaaatcat
    17701 cctgggatgc ggtatcatgg cctcccctac cttcaagtca aaaaacgccg cacccttttt
    17761 ctcgatcccc cgtctcagat cgccgtcagt gataataccg agaatttttt tctcggcgtc
    17821 agcaacaatg gtgacgccga ggcgtttaga tgatatctcc ataacggcgc gtaacatcgg
    17881 ggtttcagga gaaacgacgg gaagggcatc tcctgtgtgc ataaggtctt tcacctttat
    17941 gaagagcttt ttcccaaggc tgccgtgggg atggaagaaa gcaaaatcct cctctttaaa
    18001 tccccgcttg atcaggaggg ccaccgcaat cgcatccccc atggccattg ctgcggtagt
    18061 ggatgcggtg ggaacaatcc caaggggaca tgcctcttcc tgtaccgaga catcaaggtt
    18121 cacatccgcc gtctttgcca gggtagaatc gttctttccg gtcacagaga taagaccaac
    18181 atcgaacctc ttcagaaaag ggagcagaca caccagttct tcggtttcac cgctgtttga
    18241 tatggcaatg actacgtcat cggatgtgac catgccgagg tctccgtgcg ttgcctctgc
    18301 agggtgcaga aaaaaagcgg gcgtgcctgt tgatgccagg gtagccgcaa tcttctttcc
    18361 cacaagacct gacttgccca tgccggtgac cacgaccctg cccctgctcc ggtagatgag
    18421 atcgaccgcc ccttcgaaac tactatcaat cttttcgata agggcaaaga ccgcatccgc
    18481 ttcggtcttg agcacttttc ttgcgatatc aagaatattc atctctcaca ctgcacttct
    18541 ttcaccattt accaatcaaa ctggcagcgt agccatcctg gagggatagc cctccggtgg
    18601 gcatcatctt ccaggtccgc aaggtctcgt tctgactctt tgagttgcct tctcgatttc
    18661 tcgagcttct cctcggcact ctttttttct ttccgccact ttccttcagg tcagtgagga
    18721 ccttttcttt gttgagaact tcttcttggt tcttttcaat cttccttctg taagaagtgg
    18781 ccttcttgca ccagtattct tgctccttct ggctttctcc tttctttgcc gtttctccct
    18841 tgtctccagc tttgtcgtgc ttcactgccg aattcccgtc atctgcaggt tgcctgtatt
    18901 tcttgacgtc ctcatttgta aagtaaggtg catcgtctgc tggcttgctc tctgctccca
    18961 tgacagaaag aaagaaaaaa acattgagaa ggattgtcca tgagattatc aaaaacgtat
    19021 gaatattttg tcctttttct ctaccgtact caaccttagt gactgaccct ctcattttct
    19081 acttcactaa tgcatcaatc gctttaagag tggcgagcat cctcgggaga tcatccagct
    19141 tcatcatatt cggaccatcg cacaatgccc tctcaggctc agggtgaact tccatgaaca
    19201 agccatcaac accgatggca actgcggccc ttgcaagagg ctctgcaaat tctccctggc
    19261 cccctgagct gctcccctgg ccccctggaa gctggaggct atgggttaca tcaaatatca
    19321 cgggataccc gtaggaacgc atgatgggca atcccctgaa gtccaccacg agattattat
    19381 atccaaaggt tgtacctctt tcacttatta agagattatg gttgccggta gaggtgaatt
    19441 tttctatgat atttcttaca tcccaagggg cgagaaactg acctttctta atgttcacag
    19501 gtttccctgt ctctgaagcg gcaaggatca aatctgtctg cctgcaaaga aacgcaggta
    19561 tctgcaaagc atcaagcact tctgaggcag actttatctc atgtactgag tggacgtcag
    19621 agattactgg aatgtcaagc ttggccttca cgttagcgag tattcttagc cccctttcca
    19681 ttccggggcc cctaaaagat tccaaggatg tcctgtttgc cttgtcgtaa gaactcttaa
    19741 agatgaggtg aagactagct gctgaacata tctctttgag tctctttgcg gattcgaagg
    19801 ctatttcttc ggactcaata acgcatggac cagcgataat aaggggtggg gcaccgcctc
    19861 ccacaaccgc accggcaata gtgatttttc gtaccacttc tcagcctctc tcctccgcaa
    19921 cataggggaa tagagacctt ttctctttca gtgctgctcc tatgaatgcc ttaaaaagag
    19981 ggtggggttc tgtgggtctt gatttaaatt cagggtggaa ctggcaccct aaaaaccatg
    20041 ggtggtcttc tatctccacg atctctacaa gctctccgtc agggcttgta ccgcttatct
    20101 tcataccgtt ccgcgtacag atacctttat atgcattatt gaactcatac cggtgtctgt
    20161 ggcgctctga aatttccttc acctcgtacg ccctgtacgc atgggtgttc tgctcaagga
    20221 cgcaggggta tgccccgagc ctcatcgtcc cccctttttc acatccctcg gacctttctt
    20281 caatcttccc cctcctgaag tcataccacc gttccataag gtatatcaca gggtctttga
    20341 tcttcatgtt gaactcggtg ctattggcgt cgagaccaca tacattcctc gcaaattcaa
    20401 taaccgcaca ctgcatcccg agacaaatac cgaagtaggg tatcctcttt tccctcgcgt
    20461 acttcaccgc ctcaattttc ccttctatcc cccgataccc aaaccccccg gggaccagta
    20521 tgccgtctac ctcggacaaa aacttgtccg gtccgtgaac ctctatctcc tctgaatcca
    20581 cccacagaag gtcaaccttc gcatcattgg ctatccctcc gtgaacgagg gcctccatga
    20641 gactcttata ggaatcctta agacccacgt actttcccac tatggcgatg ctcacctcat
    20701 tcctaggctc cttgagcttc ctcacaatgt ccttccacaa ttcgaggttt ctctcccggg
    20761 aaggcaactt cagcttgctg atgatctgct ggtccagtcc ctcttcgctg agggcaaggg
    20821 gcacttcgta aatggtctcc acgtccaagg cattgattac cgtgtcacca tcaagattac
    20881 agtggattgc gatcttcttc tttgcctccg gaggtatggg tctatcggtt ctgcaaagca
    20941 ggatgtccgg ctgaattccg atggctcgca gctcccttac actgtgctgg gttggctttg
    21001 tcttgagctc accagcggcc tttatgtagg gcacaagggt aagatggacg tacattacgt
    21061 tttccctgcc cacatcgtag cgcatctgcc gtatcgcctc gagaaagggg agactttcga
    21121 tatcgccgat ggtacctccg atctccacga tcaccacatc attgctgcca ctcaccgact
    21181 tgatggcctg cttgatctcg tctgtgagat ggggtacgac ctgtacagtg tctccgaggt
    21241 aatctccttt cctttccttc gtaatgacat tgtaatagat ttttccggta gtgtaattgt
    21301 ttgcctggga cgttctcata cgagtaaatc tctcataatg gccaagatca aggtctgtct
    21361 cggcgccatc atcggtcaca aagacctctc cgtgctgaaa gggactcaat gtgccggggt
    21421 caacatttat gtaaggatcc aatttctgga ttgtcacctt gagccccctc gcttcaagaa
    21481 gggcgccgat tgccgctgct gcaattccct ttcccaagga tgacaccacg ccacccgtta
    21541 tgaagataaa cttagccatc tctccaccct ttccaggtcc aagggagtat caatccccat
    21601 ggtttcccat gatgttcctt tcactttgat cttaagccca ttttcgaggg cacgaagctg
    21661 ctcaagcttt tctatctcct cgagaaccga aggcttcatg ccggtaagtc ttaaaaggat
    21721 atcccgccga tagctgtaaa taccgatgtg cttgtaaact ttaaaaggtg aaccctgcat
    21781 gccggaagcg ctcaggtctt tccatgcatc cctgtgatag gggatgggtg ccctcgaaaa
    21841 atagagggca aaaccgtcac ggtcgaacac agccttcacc acattggaat ccacaacttc
    21901 ctccggcctc tctatttcct ttaccagtgt gccgatatca gccctggaat cggaaaggag
    21961 acctattgca tcatcaatca tttcagacct tatgagaggc tcatcgccct ggacattcac
    22021 aattatatca cagcttaatg agcgggcaat ctccgcaacc ctgtccgtcc ctgacacatg
    22081 ccccgcagag gtcatcacca cccttccccc aaaggagaga accttctcga atacggtctc
    22141 actgtcagtg gctacaataa cctcctcggc agtcgctgca cccttcgagt tctcgtacac
    22201 gtgctggata acaggaattc ccttgaggag ggcaaggggc tttcccggaa agcgggttga
    22261 ttcataacgg gcaggtatca caataaccgc agacatgact tagaactaat tatatatgat
    22321 aattgacaaa ttttgaatcc ctatttacct tttctcactc aatctgacat aataagccct
    22381 aatttgtccc ttgggagagg agggattgat ggcaactgtt ctcaggcttc caggctcatc
    22441 ctggaggcga agaccgaacg ctcattctct gccattcatt ccttctttga ttctcgtgca
    22501 gtgttaagga acgtgatcga catgagagca aatccggtct gatggtgaga ctccgaagga
    22561 atccttatca aggtacctta tttggatttt gaaagtttaa taagccgtct cctcctggca
    22621 gccttgccga ttctcgtggc aataaccttt catgaggctg ctcatgggtt tgtcgcgaat
    22681 aaattcggtg atccaacggc aaagatgatg ggcagactca cccttaatcc cctggctcat
    22741 atcgatccct tcggcaccat tattatcccc gtaatgcttt tcattctctc gaaagggact
    22801 ttcatgttcg gttctgcgaa acctgttcct gtcaacttca acaatctggg gcatccaaga
    22861 agggattctg cccttgtgtc tgcggccggt ccggccacca acgtgatcat tgcctttctg
    22921 agtgtattgt tgctcatact aagttataaa ttgttccccc aaaccgcacc tcaggaatta
    22981 ttgagcgaaa gggtaatcgg tcctgtccag gcgatgctcc aatacagcat agttttcaat
    23041 atatttatcg cagccttcaa cctcattccc attcctcctc tcgacggagg gagaatcgcg
    23101 gtaagccttc ttccgctcag gcacgcgtat cagttcagca gacttgagcc ctatggaatt
    23161 ttcattgtaa ttatcctctg ggtgacgggt atcgcacaga tcattatcag acccctgcaa
    23221 ctgctcattg agttcatcct ccaggtgttc cttatcccat tcggcgggtt catgtgaaag
    23281 atcgggtctt aagcggaatg caggccagcg ggaagctcca tctcggaaat ctcgtggggg
    23341 ctctccaaaa ctgggtcagg ctccaaaacg agtatgactg ctactatttt attgccgact
    23401 ggcacgccct tacaacgggg tatgccaacc cccaagcgat aagggagtct acgcatgata
    23461 tcattctgaa tttccttgcg gccggtctcg accccgaaaa gtgcaccatc ttcatccaat
    23521 ccagaatccc tgaacacgcg gaacttcatc ttctcctctc catgatcacg ccccttggat
    23581 ggctcgaacg cgttcccacc tataaggaga agattcagga gctcaaggat cgggatctgt
    23641 ccacctacgg ttttctcggg tatcccctgt tgcagacagc ggatattatt atttaccggg
    23701 ccagatatgt cccggtgggg gtcgaccagg tcccccatct cgaaatctcc agggaaatcg
    23761 cgagaaggtt caattacctt tacaaggagg tcttccctga gcctgaggcg cttttgacag
    23821 agtttccaaa agtcccgggt gtcgatggaa ggaagatgag caagagctat gacaatgcgg
    23881 tgtatctttc tgatcctccc gatgtcgtgg aacagaaact aaggacaatg atcacggatc
    23941 ccgcgcgcaa aaggaggacg gacaagggag atcctgaact ctcccctgtg tatcacctcc
    24001 atcaaatatt ttcttcaaag gaggaacagg aacacgttgc ccacggatgc cggaccgcag
    24061 gcataggctg tatcgactgc aagaagattc tcataaaaaa tgtgtttgcg gttatggagc
    24121 ccatttggga aagacgcaac aaactcctcc acaatccgga aatcgtctat gagatcctga
    24181 gaaaagggac tgaaaaggca agtagggttg ttcaggaaac catggagctt gtgagagaag
    24241 ccatgggact gggaaagtgg tagccacgca ggagaatccg tcaaggcaga ttgcgcactg
    24301 gctggtttgt ccctaaaaaa aatttttcac tgggaagtcc tcatacgcca tgcgtagatt
    24361 ttttcatggg gtaattcgta taatttatcc ataacgtaat tcttatgtag tacggacgaa
    24421 atgaggaaaa tggagtctta gcgtattctc cattttcccg gatttaactt cgtgagtttg
    24481 ccaccttgaa gcaaataact cttgtaaggg agaagggtac atggatacca cggtagggaa
    24541 caagaaactc aacagctgga tcactgaagc agcggcaatg tgtcagcctg acgcaatcta
    24601 cgtgtgcaac ggatccacag aagaatacac cctcatgatg gaaaaattga tagccagcgg
    24661 cagtgccacc ccccttaaga aacgtcccaa aagctatctt tttcggtctg acccgagcga
    24721 tgtggcgcgc gtggaggacc gcacctttat ctccacttca ttacaggatg aggcagggcc
    24781 tacgaacaac tggatcgatc cggtggtgct cagggagact atgaaggggc tctacaaggg
    24841 ttgcatgaag ggacgtacca tgtacgtgat tcctttttcc atgggtccca taggttctcc
    24901 tatctcaaaa ataggcgtcg agatcactga cagcccctat gtggtcgtca atatgcatat
    24961 gatgacccgt gtcagttcaa gggttttgga acttatgggc actgacggag acttcatccc
    25021 ctgtctccat tcagtcggtg cgcctttgga gaaaggccag aaagatgtgc aatggccttg
    25081 cgcgcccatt gagaaaaagt acataagcca tttccccgag gagaatctga tctggtccta
    25141 tggatccgga tatggcggca atgcgctcct cggcaagaag tgtttggccc ttcgtattgc
    25201 ctctgccatg gcaaggagag aaggctggat ggcagagcat atgctcatcc tgcgcctcac
    25261 gaatcccgga ggaaagcagt atcatatcgc agcagccttt ccgtcagcgt gcgggaagac
    25321 caaccttgcc atgatgcggc cgtctctcaa aggctggaaa tgtgaatgca tcggcgacga
    25381 cattgcatgg atgaagatcg ggacagacgg acgcctgcac gccatcaatc ctgaaagcgg
    25441 attcttcggc gtcgcccccg gaacgtctta tgacacaaat cccgtggcaa tggatacggt
    25501 cagggaaaac gttatcttta cgaactgcgc cctcactgat gacggagaca tctggtggga
    25561 ggggatgagt gacgagttgc cttcgcacct gatcgactgg aagggccggg actggacccc
    25621 tgcgagcaaa acagacgcgg cccatcccaa tgcacgcttc accgcacctg cttcccaatg
    25681 ccctgttatc tgcaaggatt gggagaaccc cgaaggtgtc cccattgata tcttcatttt
    25741 cggtggtcgg cgcggcagcg ttgtgccact ggtccacgag gctttctctt gggaacacgg
    25801 ggtctttctc ggcgccatcg cggcctctga aactacttct gccatcattg gcgctgtggg
    25861 caacctgaga agggacccct ttgccatgaa accattctgc ggatatcaca tgggcgacta
    25921 tttccagcac tggctcacca tgggcgaaaa gctcggcagc aaggccccga ggatctttta
    25981 tgtgaactgg ttcagaaaga atgaggacgg gaagttcctc tggcccggct tcagcgacaa
    26041 cagcagggta ttgaaatgga tgtgtgagcg cgttgacgga aaagtgggag ccaagacttc
    26101 cgccatcggt ttgctgcccc aggaaggaga ccttgacctc tcaggtctta ccatatcccc
    26161 cgagaatatg aaagagctga tgagtgtcaa cacttctgag tggaaaaatg agataccgga
    26221 cgtggaggcg catttcgcat ccttaggcgg ccgtgttccg gaaaggctga agacccacct
    26281 caagggtctc aaggagcgtc ttggataaac ccgcactgat cggattgtct ctacagcttt
    26341 aaccgcgtgg aaacgaccac atcgaaaaaa acactggtca atgtattggg cgtcgtgtat
    26401 gtccatacaa agaccagtga cggtggagat ctttatctca cgcgccatgc cgaacccttc
    26461 catgagcatt ttcaaataga gaactggtac gaaagagact ggttcaatgc caataagata
    26521 cgccttatcg gtaccagttc ggtctataaa gtacccacaa aagaggttgc tggcaagagc
    26581 cttgagctgg tcgtaaagaa ctgccgtgtg ggcgaagatg tgcctatcga tacccatacc
    26641 ctgcaggaat tttgcgatgc cgagttcaac agcccctggg aggagttctc cctggttacg
    26701 gaactgttgg ataacctcta cgggccaagg gaaacgaaga tgaataccca gctgcccatg
    26761 gcaatctatg tgccccctga gaagatgcag gcctggcaga gcgggaggtc acggtcgagg
    26821 atcaaccgta tcagggccaa gcacccgggc attgatattg atatcctgaa acaatataag
    26881 ctcatctatg aatggataaa aggtcataat ctcatggaag tgtttgcaca catagatatc
    26941 aaccatgatg aactggtcac tcaccttaag aaaattaatt ataaggccct tgcggatatg
    27001 cagaggaagg gttacctgat ggctgacatg aaacctgaac atattatcat cagcgaagct
    27061 gacactgagc acatcgtagc aataggcatg actcaggaga gaaacgccgc tgataagcag
    27121 gtcgatatag tgtatcagtg cattcacgag ggaagctact caatcattga ttacgaattg
    27181 ctgctccgca cccctgaaca tgatgaagca gtaaagtctt ccaggagaca ttcctatctg
    27241 gatgaccagc gccaccgctt tgacccgact cctctccctg cccacctgtc ccacatggaa
    27301 atctttggag tgccctacct gtacggacat gcggagagca ccggcggcag cctctgggtg
    27361 gtgggaagaa acgcacatct gttcgattat tttctccctg aacgttggag aaagaccccg
    27421 gccctcagat tatcccacac caaagaagtc ttctatacga tcacaaagga caacattcac
    27481 ctcgtatggg agacatcacg ggttggtgaa atgcccatgg gagatgaaga ggggtcccgg
    27541 gatcccaagg ttcgtcacta cggcatcaat agtccctttg aggaatttgc catcgcccat
    27601 acccttaccc aactcggtat tcccactgtc tatgcaaggg caatctacat gaccggaacc
    27661 acaaaggtgg aggcatccac tgatctcagg aagtacgaat cccacaggga tatccttgat
    27721 ccagaaggca accccgtgct tcaggagaac cacaactata tcaccattcg tggttattac
    27781 aacgggcctg atcagtgggt tgcagagcat gaagaggcgc tctacacccc ggtcgatctc
    27841 tcaaaggccg tttacatcaa catcatcagt gaggaacaat gccaggactt tctcgatgcg
    27901 gtaaaaagaa aattaaagct gcacggtttt gacggaacat cattgaaagt gaacgatctc
    27961 cttctggcaa ttgatgataa gcagcagatc atggaaaata gttcaggcga tccggtggtg
    28021 attatctgta atttcgaact catatggaaa atgtctgagt agcattgagt cagtacaatt
    28081 gacccatatc catacccctg agtattcata aaactaaccc attaaggggc cctcccagtt
    28141 taaggcaaga cactctgcca aaaacagggt ggaccccaaa atctaaagag tgtatgacta
    28201 atcaaactaa aacctcatcc tcaccctgta agtaagcttt gcttccttgt ccttctctac
    28261 aggaacgtta aattctgcgg tagaagcctc tgttttctta tgctcatgcg atgaactgag
    28321 catcacccag tctccaggga tcggctcgat cactttcacg acaacgttct ctttcttgtg
    28381 gttcctgagc gatatctcaa agccagcttc gtacgtatct gaagcgatct tcttccaatc
    28441 tgtctgtttt cttgcgccca caacgtcaaa cgcatcgccg agttttatcc tgatcttttc
    28501 atccttcggt gtatggtcga tggagtcctc accgacgaac tggaggcttc cttcattgtc
    28561 cttcttgtat actctcaccg tacctttggg caaaggaatt ccgaggttat ggtccttcgt
    28621 gttcgctatc tctgcgaaaa cgccgacctt ctggttcgag atcttttccc cgtaacttga
    28681 ccggtagtag tactgggcgc cgtagtaaac gagttctttc ttcaccccaa tattatcagc
    28741 gttcacaagg ctgatctgtt tggtctggtt gtctttgatt gttgccagtc tctgtaaagt
    28801 gtagatgtgg tattcgaaga actcctcttc cttgaactga ggcgcaggtt ttgccgccgc
    28861 ctccgcagcc ctcatcattc ccttcttgta ctccatctca tccttcgccc tgtttacatc
    28921 gccagcaaca agcttgatct ttgcgtcctt gtaagttgtg ccgcttctgt tgtcaatggt
    28981 gacccatccg gaaaggtccg ccctgtcatc cttgtcattc aatgtcacca catagtcgga
    29041 cctccagtta atgccgtttg tgaggtatga agcctcaacc ttttggagcg aagcaaggct
    29101 gttctcgatg agccagacca gtgtcggttt tgaaataaga ctctcgggga cccccggaaa
    29161 tattatcctc ccgggatggc cgaaggtgat ctcatcgccg atcctgaata tcggtcctcc
    29221 attgttcgaa agtagggtag cggtcacgat ctcttctctt tcggtatatg ggtttttgta
    29281 atagagcttc acttccttgc ctacgtactt gtccatgagc ttctggggat tcagtaaatc
    29341 atattcataa ttctgctcaa ggatctgcag gctgtcggca ttcacaaggg atttgatatg
    29401 gacactcgtg ggaattatct gcgacgccac atccataaac ttcagctcgc ccattccttt
    29461 cgaaagcttc aggcttcttt ggtccttcac aaggccgaga ttcacattat agatcgtgac
    29521 ggcaacgcct gtctgatctt cgagccctgt cgaaaaagca gctggtctac tgtccttttc
    29581 cggagcagcc acagaaacaa gaccgaagca taagtacaga actgcgagta ctgcggaaaa
    29641 atactttgct ttcatggtaa ccctcctttg atgttttctt ggttacttag acaaaggagg
    29701 cgagagcaaa gttcccgggt ctcgaacaag gatcatttgg gcaggagttg aatgcaccga
    29761 cgagggcgat agtaatcacg acagctaatg ccaacagaac tgggaccttg aaactcataa
    29821 ttgtctctcc tttctgattt gaggtgactg gcgaaaaatc ccccaggagc atgcggatat
    29881 ctaagagata ataagatgag gccctgcacc cgatcgaatt ttcctcattt tcctttaacc
    29941 ataaaatatg cggcctgtca attgcattgc agtgcgttaa tcaacgcaga taaggcctag
    30001 aagtgggtca atttgacgag ccggttaaca ccgaatatgt tcaaatgcaa aaaaaaacca
    30061 ctgcactcat cttgacaacg atgagcgagg tgttattatc tgattgaagg actatgaaat
    30121 gatcggaggc agctcttttc cataacatca agcctgtaat aatgaggtaa aggtatgtcg
    30181 attaagaatc tcgacgtttt tttcaatccc caccggatag cggtcatcga ggcgagcgac
    30241 gacaagcggt ctctggggta ctatgccttc aggaatctcg tgggaaaagg gtttaacggg
    30301 gttgtttacc ctgtgaatca acgccgtgag gcagtgcagg gtgtagaggc acacaagacg
    30361 atcctcgcca tcccgcatca gattgacttg gccatagtcg cttctcctgc cgctgaactc
    30421 tacaccgctc tggatgaatg cgggcagaag gacgtcaaag gtgtcatcat ccttgccccg
    30481 cactttgagt cccccggcgt caatgctgaa gagcttgaaa accagatcag gaggctctcg
    30541 aacatctacg gcttcagggt catgggtccc aactccttcg gcttcctgcg gccaagcaaa
    30601 aaccttaatg cgagcctttt cccccatatt ccccggaagg gcaacatcgc cttcatctcc
    30661 caaagcggca tcttctcctc tgtcttcctg gagagggcca taagcaaaca gatggggttc
    30721 agccatttca tctcggtggg agaaaagatg gatatagact ttcccgatct gatcgactac
    30781 cttgggatcg accctgaaac acgcgcgatc atcctctatg tccagaatat caggagcgga
    30841 aggaggttta tgaccgcggt gagaagcttt gcaagcagca agccgatcgt ggtggtaagg
    30901 tcagggcggg cggaggtttc aagtcggata ttcctgaccc attccggggc gctcgcaggg
    30961 gaagacaagg tctatgaggc ggccttcagg agggccggcg ccgtacgggt atatgagatg
    31021 cttgatctct tctacatgac cgagacgctc ggaaaacagc aacggccgcg gggaaagcga
    31081 ctggcgatta tcgccaattg ccagggtccg gcctctatgg cggtggacgc ccttattccc
    31141 ttggaggggg aactggcagc gctagggaag gaagtaatgg atgaaattga agaacaccag
    31201 accctcagaa agaggataaa caaccctgtc tatcttctgc cggatgtttc acccgaagac
    31261 ttcgaactgg cggtgagaag ctgtttgaag gacagcgggg tcgacggcat tttggtgatc
    31321 tatatcccct ttccctgttt tgatccaagg gacatagccg ccgccgtggt aaaggccgcg
    31381 aagtcagctc cccacatccc cctgtttacg gcatggttgg gagacgaaca ggtgctccgg
    31441 gcccgcgagt tcctcagcag caacgagata cccacctttg taactcccga gcaagcggtg
    31501 aagagcttct tgtatatgta ccggtatgac tttaacctca aactgttgca cgaaactccc
    31561 gaggtgatcc tcaaggactt tgtccccaat atcgacaagg tgggggagat catcaggaat
    31621 gccgcagagg gcggcaggcg catcctccgc ctggacgagg caaaggaaat cttcgagaca
    31681 tacggcattc ccatggtaga gacggtgagg gcggtcaatg aagatgaagc tgttcggctg
    31741 tcagaaaaga taggttatcc ggtggtcctg aagatcgatt ccgaaaggct ctttcacaag
    31801 ctcgaaaaag gaggtgtcgt tctgaacctc gggcatgaga aggcggtcag ggaggcgtac
    31861 cggtcactga agattatagc gtctcaggtg ggtgatactg aggcagacgt gatcattcag
    31921 ccgatggtaa taaagaaggg atacgaactg gtcatcggca caaaaaagga ccctagtttc
    31981 ggcgctgtca ttgtcttcgg aaccggaggt gaactcctcg atgcgataaa ggattattcg
    32041 atcggtcttc ctccccttaa tcaggccctg gcgcgacaga tgatggaaga gacgaagata
    32101 tgccagtatc tgcaaagtct ccctgcctgt caggggattc tgcggtttct tgaagagata
    32161 ctagtcaggt tttcccagct gatcatcgat tttcatcaca tccgagagat tgagatcaac
    32221 ccctttctcc tttccgacga atacggtatt gccctggatg gcggcatcct ccttgaagag
    32281 gaggccctga cgaaccatcg cagagtcacg ggcgacctct gccctccgca tctgtcgatc
    32341 tgcccgtatc ccttcaaata tgtcatggaa agaacgcttc gtggcgggac ctcctgtcac
    32401 gtgcggccga tctgcggaga ggacgagccg cagatctaca agtttttcaa gaacctcgcg
    32461 gacgagacta ttatctaccg gtttggccac caacttactc atatacccca tgagacgctt
    32521 gtcagatact gccaggtgga ctatgatcgc gaactcgcct ttgtggccga gagcggagac
    32581 ggaaaggaca ttatcggcga tgtcagaatc agcaagctcc cagacctcga aaatgcggaa
    32641 gtgtcaattc tgattgccga cgagtggcag ggaaaggggg tgggaagtat gctgatggat
    32701 tactgtatag atatcgcgag gcagactggc ctgacgacac tctggatgga gatcctgaag
    32761 aacaactcac gtatgctggc cctttcccga aaatacgact tcaaacaggc gtatgacgac
    32821 gaagacatgg tgcgggtggt ccttaagcta tagagcttgg tagcggggag agaatttgaa
    32881 cctctgacct tcgggttatg agatgagttt gaattatata tcagtctgtg ataggctgga
    32941 taatccttta aatgacagtc ccttaaccct tgccaataaa ctaccatttg taacattttc
    33001 aaggttagta taatctgttt tggtagaact atggttgaag aaaaaggggg ttggttatcc
    33061 aggccccttc tgtttgtttc acggacaatt ccgtatttca aaatattgca gctccgtgaa
    33121 gagtaagcca gggaattggc accttttgac agacctgtga ggcatatgct acagccacaa
    33181 acaaacattt caatgttcag aactttcctc acatctcctc ttagtaacag atatgtgccg
    33241 cgatcctgat cactcttcgg gttacgggcg gtaataatta catctgatgg cctgctgcgt
    33301 tctcaggaaa gaaaagctgt taaactgctg caatctgcca tgtctccatg agcaaggcct
    33361 gggaaaaaac ttaaggaata aaatgggggg tctatgagga aggaagggga tatttggata
    33421 actcttgctt ctctttccat cgcacgggga acataatggt aactgtcgtg cctttgcctt
    33481 cttcgctttc gagcaccatt tcaccgagat gctcggagac aatctgcttt accaagggca
    33541 gccccattcc gaatttatgc agttttgtag taaaaaaagg atcaaagact ttttcacgaa
    33601 tttcttttgg aatccctgaa ccggtatccg agacgatgac cgaatttttg tcgccgtccc
    33661 cggtgctgtc aactgatatc tcgcctcccg ctgatgttgc atctatcgca tttctcatga
    33721 tgtgaaagag tgccgcacgt aaaaggtttt tctcgaggtt cattttcaga ggctggggtg
    33781 agagattgat gttgagacgt aatcgcttct gggatgcctc tttttctacg agagagacga
    33841 cactccttat cacctcgtta atatcctcgt gtttaaatat gctctgtctg cttctcagga
    33901 gacattcaaa gttctccaca acgttctcga gtcttcggca ttcctcaatg atctctttca
    33961 gtttatcttc catttccggc gtgagctccc ctttagcgag gacccttttg caggtacagc
    34021 caattatcat tgctggattt cgcacctgat cagcaaccgt caatcccatc atcgccatcg
    34081 tccgctcagc cacaagtgtc tctaattcct ggttcatctc ggtcaattca gcattgatct
    34141 tttcgatttc ctgctcatct cttgacatct tcagataaag gttataaatg acgatgaaga
    34201 aaaggctcac agaaccgacc aggaaaaaag taaggttatt gaaacttccg ctgaccggct
    34261 cgacgtacct ccagatatgc aggtttccag tagagacaag aagaggtttt gcgatatgac
    34321 cgacggctct ggaaatggaa tacatgacgt aggacgacga aacccatacc gtataatacc
    34381 acagcgcatt ctctctgttg cgcctgtaaa gcttcaagct gacattgaga caggagagcg
    34441 agagcgctat catgacgaga gctccggcaa catcagaaat gataattggc aggaaaggca
    34501 aaagggccgt tacagaaaaa ggagtctttg attcctcttc tttttccagg aatttccgga
    34561 gaccgaggta aaagaaaaac atcccgagat caaggagcac atagtcgcat cggcccacat
    34621 ataacaggta gtatttgccc tcaacaacca caaattgctt taaatagttc caaccgtccg
    34681 tatcccctac aatccagagt tctgcaaaat ggctgagagc aacatctaag tcccagacca
    34741 tatacgttat cactgctgca aggatatatc gccagccctg ctccctgtgt aaacggttac
    34801 gaactatggc ataaaggacg aaaatgcacg cgatcaggaa aaagatccga cccagttcgt
    34861 gagtataaat tgcaggatat tcatgcgaaa caaatgccgc agcctttgaa ggaacaatca
    34921 agaaaagaat aaatgagatt aaccagttca tgctcattat tatcatacta tacattttct
    34981 atttgtctga tacaatagta atagcttcag agtgactctt gaagtcattc agatggtaaa
    35041 ttaggggtgg agtagtgcaa actctgaata tcggaagaaa gctcgtactt ggtttcggag
    35101 cgatcctggt agtgatggcc atgctggtca tggctgcgct tatgaacatg agcgcccttc
    35161 atgaagacct cgtcaggatc gtgctcgtaa acaacaagcg agtgaccatt gccaccggca
    35221 tggccacgat tgtccgcgaa gaggcaattg ccgttcgcaa ctgtttcctc cagagggaac
    35281 ggactgagga aatgataaaa cggatcaatg attacaatgc aaaattcgat gaagcattta
    35341 cgcaggtcga acagatgact tcgagcgatg acgcgaaaga gcgcgagctc ctggtattgg
    35401 tcaaggaaag ctggaccgca tcaagggcgt tgaacgagcg cactctggcg ctcttgaccg
    35461 caggtaggca gcacgaggcg tttgcgctgt atgaaaagga atcacgggcc gccgtgcgac
    35521 aggctatcca ggctaccgag gatttggtga aacatcagca gacgcggagc gaaatgcggt
    35581 acgatgcggc aacgcggcat taccgggata cacagctctt catgatttcc attggaattg
    35641 ccacttttct ccttgtcatt attatagcta tacgattctg acacgaaaca ttgtccggcc
    35701 tctgcaatta gcacactaga gagccgggaa agggactcga accctcgacc tgctattacg
    35761 aagtattttt agacctttcg taactcgcca atctttacct ataaaattaa tttttaagca
    35821 tttcccccaa aactgctctc ggactttacc ggaccttatt tatacaatga gcacagggca
    35881 agagagttag taggggccag ttatctaggc cgtttctgtt cggtacaccg accacgtcac
    35941 accggcacta ttttcatggg ggtgggatta ccgatcaaac ctcgcattct tccgagagaa
    36001 ctgagtttgc ggtgtacatg tcgttatcgg cacatgggat agatcaggta ggtcgaactc
    36061 acttgcatcc tgaaaggcgc accttaccag atggtcaaaa aaatgaagac aataagtgtt
    36121 aatcaatgca gatagtgatc cttatagtgt cagtcctaaa attataatta atagacttca
    36181 ctataattaa aacatcaagt tgacaaggcg acttttttcc tgctacatat caaataaaag
    36241 ggaaaggaga agtttaagta tgcctacgat ggattttcgg ggaaagcaga tcgagataga
    36301 tggggaaggt tacatcgcca atcccgatga atggagcaag gaactcgctg agcacatagc
    36361 gaagatagaa ggcatcgaaa tgacttttga tcactgggac gttgtcaatt tcctcagaga
    36421 ctattaccgg aaatatcaga tcgctcctat gataaagatg ctggtaaagg agatcggaaa
    36481 ggtcaagggg tcggagaagg ggaatacgag gtatctttat caactctatc cggggggtcc
    36541 tgcaaagcag gcatgtaaga ttgccggact tccaacgccg acgggatgtg tataatggta
    36601 attaatcaac aagaagaatg aagttatgcg gtttgcatgg ttttgtataa tcttgtttgg
    36661 aattttcata atcggaggct tagcgacacc gaaaccggat ttgcccttta tcataatggg
    36721 attcggcggt ctagtgataa ttgcctatgc tgtggcaaaa aatcgttatt ggggcaagcc
    36781 catagacttt aaggaactac ctgtgggtga gatattctat gtagaaaaaa ttttgcgtga
    36841 cggctatgtc attatcagtc aaccaaaaaa acttccctta atgataacgg ggtttcctac
    36901 agaactgatg aaagaaggac gaagctttaa aaaaacgaaa gaaatgtcat atgaggtcat
    36961 tgcctggtaa aaccagtctg acccttatct tgcggactcg ggaggggtaa cagtagccca
    37021 aaccatccct aagctaaaca ctcttccctg tttcgtacct cactcataaa tatgttgact
    37081 tctaacacgt aaccttgatc tgctccgtag gcatccaaca tttgttccca aaagaaacct
    37141 ttcgctgtgg caaaaaagtt acagtgttgg cttcaggagg aagaagtttt tatcgatctt
    37201 aaggaatcga tagaatctct atccttatcc caatctctcc cttcgcacct tgaaaatcga
    37261 tatccttctc tatcacctgt cccacagact tcaatctttc atataattcc ttcaattttc
    37321 cggagtcgat ctccgctgta aggatatcct tattctcata agattctttt ttgacaacct
    37381 tacccccaag ttctgagatg tgcctttcaa tctctttttg ggcagactcg acgtctttca
    37441 cgtgaacagt aacccgaata ctctcctttc tctcctcagc cattgccttc atcgttggtg
    37501 cggcaggggc agcttcacgc tttgcctctt cctttgcgag agtgccgact gaagatggcg
    37561 agacatcctt tcctgaagcg ggggcagcgg ctctcctctg ctcctgcgat aactcagcct
    37621 gtttcatcgg ttccggggcc ttaggaactt ccctcacggg ctcgggtttt tttgatggaa
    37681 caggttgctc agccggcttc gaagataacc cctccacact tcctctctta tctaagggga
    37741 ggccaggtgg tgttgcgcct ttgtcctcct catattttag gggcggcagc tgttctcttg
    37801 gagaaacctg aggcgtagcc tcttcagtag gcgcctttgc aagtttgatc tctggctcaa
    37861 tggtcctgaa gacataaagc gttatcaggg ctatcgcgat ggttgcgatg gcaccgaccg
    37921 gcagtttaat gtgaagagga tagaatagtt tctggaatag acctttcctg ggtttggtct
    37981 ctgcgcgaac tctcgccatg attctttgag tcatccacgc aggaggtcca acggcttcaa
    38041 ggttctttgc atgtttaatc gtttttttga tatcagccag ggcggttgca cattccccac
    38101 atgacttaag atgctcatcc atagtcacct tttcctgagg tgagaggatg tcatcgatat
    38161 actcagaaag tttttcctgg aaattacggc attccattta cagttccccc aagactttct
    38221 tcaggcagtc tttcaatgct tctctcgccc tggaaaggcg cgatttcaca gtaccttcag
    38281 gaatcctgag catatctcgt atctcactat aggaaaagcc attgatatcc ctcaggacaa
    38341 ccacctcacg gtattcttca tcgagagccc tcacacaatc ccgcactttt cgttgcagtt
    38401 cttcttgctc aagtcgctca tcgacaggtg gatcgtctga tgcaacctct acacttaaac
    38461 taccttcgtc ggtaagaacg gggtcatcca tggaaagtcc ttcatgatgg ccaagggttc
    38521 tgagctgttt aagcctgttt ctggagagat tgaccactat cgcataaagc catgtagaga
    38581 atcgcgattt tccttcaaac tccctaatgc ccttatacgc ggagacaaac gcatcctgta
    38641 caatctcaca ggcatcttca tagcttccga caatcctgta tgcgacattc agcatctttt
    38701 tttggtattt ctccaccaat ccttcgtagg catcgagatc tccttgctta catcgggaga
    38761 ccgcttcatc atcttcatcc atccctgcat attcatttct atccatatat tagacaactc
    38821 tcagggttct tctgttccct gagtcccttt ccggtaattt taatgagagg gcgctttaag
    38881 tatcaaggga ataaagcaga cgtccgtcac caaggggttt tggtctgcga aggatcttgt
    38941 ccgtccacac gatgcgggca tggtatttgt tgaagaggtt caagtctctt tccagagcta
    39001 cccgaaagtg ctggcaggtt cagtacgatc cattgccaca cactttcata gtatcccaaa
    39061 ctctgccatt ttcagcttta tgccgctgta ttcactgtcg tccgcctcaa taaagccggt
    39121 atagttctta tctatggatt tcactattgc agcgccttca ggggtagagt cattgattgc
    39181 aacaagagcc tgttgtatgg tgcgcacctc ttttccatcc agggagatgt tgcagcacac
    39241 cccaaattcg gggatatcat ccgagcgttg gaggatcctg agtccctggt ctatgaactt
    39301 atacgcagtg gcttccataa cacttcccgc atcaaagtcc cccttcagca ctgcccttgc
    39361 ggcgtcgtcg tgattgccga gaaaggaata atacttcaga tcgtcaatcc cgacccccgc
    39421 atctttcagc atggtcctcg ggatgatatg tcctgcagta gatattatat tggcaaaggc
    39481 aaaggttctt cctctcatgt ctttcagcga cgcaatatcc gagtcagcac gtgtaatgat
    39541 cgcggcatgg tgatacggcc tccctttcct tagcgccctc gcaatcactt tgacactgcc
    39601 gtatcggctg tttgccttaa gatatgtggg cgcgcccatg aaacagagtt gtgtgacatt
    39661 ttgccctata tcattaatag cgccctcgaa atcaactccc accttgagct ccaccctttt
    39721 ccccatcctc ttgccgagat aattcacaag cgggccaaac cttctgaaca tttctgcggg
    39781 agactccagc ggaatgaccc cgaatttcaa ggccgctccc tttttctcgt gaaacctgaa
    39841 ccgttccatc tctgttctca ggagttcgga atccttctgc aggtccctaa gggtattgct
    39901 cactctgaaa atgagcctgc gattctccat gggtatgtcc tttacccctt caacactgct
    39961 gagtatgctt tttgagcctt ttttatgctc cgacaatgat ctcgagattt gtcgacttct
    40021 gtcagccaca agctcaatgg cagcaataat ctgcttactg ctttctgcct gttctccggt
    40081 ggccttattc acctgatggg acgcgtccct catcttttct gctgcattcg taatcataag
    40141 cgcgctcttg gactgctctg cggtggcctt tgcaatatgc cctgtcatgt cccgtacgcc
    40201 ctccatagcc tccgtgacaa gacgggcagc ctttgcctgt tctacggtag accgctctat
    40261 ggaaagcgcc atttcggatg atattcttga actatccagt atctttttca gggcatcccc
    40321 cgcttcctgc gcaagcatga acccctcctg gacggacatt attccttttt ccattgcccc
    40381 ggaagcgtta gtcactccct cctgcacgga ttctataagc gagacgatct cctttgtgga
    40441 gagtgaggtc ctttctgcca aatccttgat ctcgtcagct actacggaaa atccctttcc
    40501 atgctcacct gcctgagcag ccaaaatggc cgcgttcagg gcaaggagag ttgtctggtc
    40561 agtgatgtca tcaatgacgt tgagtatttt cccgatctcg tcagaccttt ttccaagcac
    40621 ctctatgaac tgcgcagtat gctcaacgga tgactggatg ttcttcatac cctggatagt
    40681 cttctctata gacgccatcc cgaacgttgc ggcatcactc gtgactttct ccgacagcct
    40741 tgcagactct tttatattgt cttccacctc ccttatgcca aatgttattt cggaaatgga
    40801 agaaagggta gcctccgctg cggttgcgag gtctaccgca ttcgacgcca catcctttat
    40861 cgttgaagaa agttcctcaa tggaagacga cgtggactct acctccctgg acagtccctg
    40921 gatattcccg ttgatgctgc ttatgctctg taccatctgc tcgacggaga cagaggtttc
    40981 ccctgttgag gccgcaagac tttccgtgtt gtccgcgatc tctgtggcag tggcattcag
    41041 ttcctctatt gaggtagata tgtccgtaat agcttcagtt tctttctcag cgccgttcaa
    41101 cacgttcttg gattcccttt ctacttcttc aaccaccctt gatatccttt cggacaattc
    41161 cttaatgcgc tggaggatcc ctcccagctc ctcaaaggac tccctgaggt gaaagcttaa
    41221 tctcccgatc tcatccttgt cgctcacctc cacatcgaaa gagaggtcac cttccgagat
    41281 tttgcgggca gcggtttcca tagccttaag gggcgatatg atccttttgt tcaaaataag
    41341 gaaactgaca agaagggaca agaataccga tactccaaag agcgctacaa ggagataaag
    41401 taccgaatcg attcccccct tttgctgata aagccctata aacgctgcaa ctgacggtat
    41461 catcatgaat aggatgatca cgatcggggt aaggagcagt ttccctgata tcttcagatc
    41521 accaaaaatg tttttcatct ttttgctccc tcccctcttc ctctcttcca tcgaacatct
    41581 atttctcttc tcagtaagaa gtttgtcaat tttggcagat gccgggagtt ttttctatac
    41641 atatcttgct tttttttatg catttcttgc agacatgact gaagctaatt tgagatgaaa
    41701 acctgggtca ggtaattttt tgaaagtggc catttcctgc cgccctggtt tctggcttca
    41761 atgtaatctt tttgaactat tgtcttaatg ctctaaaaga aggaggggac attccgcaca
    41821 aaaaggttta ggtttacaaa aaaactttcc cgtcgcaaca aaaagtgcgt gatactcatt
    41881 ataaagcctt acatctcttt tcagggacaa atggaagagt tcctgaaaat ctgagtaggg
    41941 ctcatcgtga cctatcatgc tgtgtcttga aaggatcctt tttgtataag cgtcgatgac
    42001 aaaaatgggt ttatcgagag cataaagcag aattgagtct gctgtctcgg gcccgatgcc
    42061 atgaaccgag agaagttgct ccctcagaat acgagcatcc tcctttccca tgctcttcat
    42121 gcttcctcca tactgctcac aaaaaaaatg tacgaaagac tttagcctct ttgccttgat
    42181 attgaaatac cccgccgatc taatgaatgc tgcgagtgta ttcaccggca tctcatgtat
    42241 cctttttgca gataagacat gtgcagcctt aagattgtga atggccttct ccacattgcc
    42301 ccagtttgtg ttctgggtga ggattgcgcc cactgcaatc tcaaagggac tatccccggg
    42361 ccaccagtgc tggggaccga aggccttgta caatgtcctg taaattgttc ttattttgtc
    42421 cctattcttg ctggtcatat gcttgacttc cataagtttt aacagtagtt tattatagct
    42481 gatgaaagaa tccttgctga aggagttgcg atgctcgata taaaatgcgt gagggacaat
    42541 ccggagaagc tcagggaagc cctggaaaag agagggatag gcgatttctt tgtgtcggag
    42601 gtgcagttaa gtctcggcca gctcgtcgaa atggaggaca aaagaagacg gttgcttaaa
    42661 gaggtagagg aactccgcaa taaaagaaat gtagtatccg aggagattgg gcggttgaag
    42721 agagaaaaaa aagatgccga ctcacacctc accgaaatga aaggagtgtc ggataagatt
    42781 aaggaacttg atgaaaccct gggtaagatt gaggaaagag agacgacctt ccttcattat
    42841 atccccaata ttccccatgc ttccgtcccc gtgggaaagg acgaaacaga gaacaccgtg
    42901 gtgcgtatat ggggtgagcc caaagacctg ggctttcctc ctcttaacca ctgggacatt
    42961 gccgagatgc tgggaatcat cgattttgac agggcgtcaa agatagcggg cgcccgtttt
    43021 gcattaatga ggggagccgg tgccagactg gaacgggcac tcatgaactt tatgctggac
    43081 ttgaatacct caaaaggata tacagaggtt tttccaccga ttctggtaaa ccgtgagagc
    43141 atgaccgcca cgggccagct tccgaaattc gaaatggagc tcttcaggat tgctgaccct
    43201 gagttttatc tcatccccac cgctgaggtc cctgtcacca acatccatcg tgacgagata
    43261 ctgaaagaag ataatctgcc gctgttctat acggcataca ccccgtgttt taggcgcgaa
    43321 gcaggctcgt atggaaagga tacaaggggg ctcataagac agcaccagtt taataaggtc
    43381 gaactcgtaa aattcgtgac accagaaagt tcatatgaag aactggaaaa gctcaccagt
    43441 gatgcggaag acatactcca gagactgtgt ctgccttaca gggttgtggc tctttgtacc
    43501 ggagatctcg gtttttctgc ctcaaagacc tatgaccttg aggtatggct gccaggtcag
    43561 cagcgataca gagaaatctc ctcatgctcg aatttcgaag attttcaggc aaggagggcg
    43621 aacatacggt ttaagcggga agggaaaaag ggaacagaat tcgtgcatac gctcaacggc
    43681 tcaggtcttg ccatcggtcg cacccttgtt gcaattctcg aaaactacca gcaaaaggac
    43741 ggaaatataa tagtccccca tgtcttgagg ccatacatgg ggatggatat aattaagtga
    43801 gcttttcaaa ctgtgccttt tgccaaagag ccgagtttcc ttcaggtgta tcctcttaaa
    43861 cctttttctc tttgccttgt ctaaccagtt gagaagaaaa cagaaggagg atgttcatga
    43921 aaaaacatat ggcgaagatt ttggtgtctg tcattatgct gtttggtttt gcggcgcagg
    43981 gtctggctga accaccggat tttgaaaagc cgcccaccag agaacagaag gaaagagtaa
    44041 ggcaaaggat agagaccttg agaatgtgga aacttaccaa ggatcttgac ctcgacgaaa
    44101 agacatctgc ccagttgttt ccattactca acaggtatga caaaaaaagg gcagagatag
    44161 aacatgccat gcaggaaagc atgaaagagt tgggggaatc cctcagggaa aaacgcgaag
    44221 gtcaattaag gggtatcctt gacagacttg agcagaacca caaaggcatg caaagtctca
    44281 aagacgcgga atgggaggaa atgaaaaaaa tcctcaccat cgaacaacag gccaagtatg
    44341 ttattttcct ccaggagttt gacagggaaa tgaggagaat cattgaagag gccaggggaa
    44401 ggcggcacga agggctaaga aaagacctac cagaaaaaca acctgcccaa gaaaggaaat
    44461 agactggggc aagaacatag acggtcagag cgaaatgaca tgcctcttct aaactatatt
    44521 taataatcaa tgtgttatta ccactcatag cttac
//
LOCUS       DATKOU010000108         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2063617, whole genome shotgun sequence.
ACCESSION   DATKOU010000108 DATKOU010000000
VERSION     DATKOU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063617"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VMU21_04980"
     CDS             <1..253
                     /locus_tag="VMU21_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUO76914.1"
                     /translation="ACCGTACHVRGAERLYNTAIKELGLSDAQDTTEDREITLEKLAC
                     LGTCSLAPVVVMNKKVYGKMTPDKLKREIKSLTKAKKDE"
     gene            246..614
                     /locus_tag="VMU21_04985"
     CDS             246..614
                     /locus_tag="VMU21_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUO76915.1"
                     /translation="MSKVTIKVCMGPGGIASGGPEVISAFESELKQANVSAEIEKGCS
                     LHKVGCLGLCSKDVLVDLVIDGNNTTYQYVKPEMVRRIVNEHIVGEKPIAEWLAGISS
                     SFENHSTASSGTYPNSACIS"
     gene            complement(806..961)
                     /locus_tag="VMU21_04990"
     CDS             complement(806..961)
                     /locus_tag="VMU21_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76916.1"
                     /translation="MEPVIKKGLSGHFGLNELAYVKVKGREIEVKILALKALKQQEIP
                     PHVDVVA"
     gene            complement(1022..1462)
                     /locus_tag="VMU21_04995"
     CDS             complement(1022..1462)
                     /locus_tag="VMU21_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO76917.1"
                     /translation="MISIKELLKKKSGDIWSISPEASVYQALELMAQKDVGALLVIEN
                     DKLLGIFSERDYARKVILKGKASKETAVGELMTRSVYYVTPDNTLEEAMALMTSKHIR
                     HLPIMNHGKLVSIVTLGDVVKRIISEQKLTIDELENYLTGGYGA"
     gene            1648..3054
                     /locus_tag="VMU21_05000"
     CDS             1648..3054
                     /locus_tag="VMU21_05000"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HUO76918.1"
                     /translation="MNTDTYKKDYGDSIAYLYGLQKFGIKLGLENTIRLLSLLNNPQN
                     SFRSIHIAGTNGKGSTAAMIASILRAAGFRVGLFTSPHLVSFTERIRVDNVEICEAEV
                     VELTREIRNIILNSQPSPAAIQDMDQTVASGQQSFLPTFFEFVTALGFLYFKRKGIEW
                     AVVETGMGGRLDATNILVPAVSVITNISYDHREFLGQTLREISGEKAGIIKKGVPVVS
                     SAQEDEVIGVITEKATELGSSLHIYGKDFFSHPKNIDIHGITFSYEGGEQMDNLRISL
                     TGLHQVENASLAIRAIDVGMKKGVIPSYAIREGLAHAQWPGRLELIENKGCTYDYLID
                     GAHNPSASRALANSLKKYFIPLYEKVVLIMGVMADKDMEAMMKPLLPLASEVIFTAPE
                     YERAASPVRLAEYAKALGVSPRVKDSVKASMELARELAGKGPQKTLIMITGSFYTIGE
                     AKSALGHECSAQSLARLR"
     gene            3192..>3383
                     /locus_tag="VMU21_05005"
     CDS             3192..>3383
                     /locus_tag="VMU21_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76919.1"
                     /translation="MIESETLDYDGATSTYTAKGHVRIYRDLGSIEADEISYNEQTGD
                     AYPEGNVIYENPDVRIKAKR"
BASE COUNT          911 a          757 c          840 g          875 t
ORIGIN      
        1 tgcatgttgc gggactgcct gtcatgtaag gggtgcagag cgcttataca acactgccat
       61 aaaagaatta ggtcttagtg atgcacagga tactacagaa gatagagaaa ttactcttga
      121 gaagttagcc tgccttggaa catgcagcct tgcaccggtg gtggtaatga ataagaaggt
      181 ctatgggaaa atgacacctg ataaattgaa aagagagatt aaatcgctca caaaggcaaa
      241 aaaagatgag taaggtaacg ataaaggtat gcatgggtcc tggaggtatc gcttcgggag
      301 gtcctgaggt aatttccgcc tttgaatcag aattaaagca ggcgaacgtt tctgcagaaa
      361 tagaaaaagg atgctctctg cataaggtgg gctgtctcgg tttatgctca aaagatgtcc
      421 ttgttgatct ggtaattgat ggtaataaca caacctatca atatgttaaa cccgaaatgg
      481 tccgccgcat tgtgaatgaa catattgttg gagagaaacc gattgctgag tggcttgcag
      541 ggatatccag ttctttcgag aaccattcta cagcctcttc agggacatat ccaaactctg
      601 cctgtatatc ctgaagaagg gttatgagat tcccttcgtg agatttgtac tcgtcaagaa
      661 tatgcaagag ttgatttcgc ataggatcag cctcttctca taaagaaata atataccata
      721 agtgaatcaa ttaggcaacg agttcataat gcacggcccc caggccggag aaaacaacag
      781 atagggctaa ttgcaggatg ccaaattatg ctacgacatc tacgtgcgga ggtatttctt
      841 gttgctttaa tgccttgagc gctaatatct tcacctcgat ttctcttccc ttcaccttta
      901 cgtatgcgag ttcgttaagt ccaaaatgac ctgacaggcc tttcttaatc actggctcca
      961 cgtgcgcatg cgtatttttg gttataagga tctttgtgcc gaaacacact cctgccccag
     1021 cctatgcccc gtatccgccc gtgaggtagt tttcgagctc atcgatagta agtttttgtt
     1081 ccgagataat cctcttgacc acatctccca gggttacgat gcttaccagt ttgccgtgat
     1141 tcatgatggg cagatgccgg atatgcttgg atgtcatgag cgccatggct tcctcaagcg
     1201 tattgtcagg agttacgtag tagacactcc tggtcatcag ctcacccacc gctgtttctt
     1261 tggatgcttt tcccttcagg atcactttcc gcgcgtaatc acgttcagaa aagattccca
     1321 aaagcttgtc gttttcaatg accaggagag ctcccacatc cttttgcgcc ataagttcca
     1381 gggcctgata cacagatgcc tcggggctga ttgaccaaat atcgccgctt ttctttttta
     1441 agagttcttt gatagaaatc atagagatat acctcccttt atgtaatgtt ctctaatgat
     1501 accccatcca taactcattg taaaggcgca ttaaggaaca ggcgccagac gttgccaatg
     1561 actttttctg gtgtctctgg taaactctct gggagttggc tccggcggaa gaggaagtgt
     1621 ctcggggaat gagaaatcac cacattcatg aatacggata cgtataagaa ggattatggg
     1681 gattccattg catacctcta cggtctgcag aaatttggga tcaagcttgg gcttgaaaat
     1741 accataaggc tcttgtctct tctgaataac ccgcagaatt ctttcaggtc gattcatatc
     1801 gcgggcacca atggcaaagg gtccaccgcg gccatgattg catccatact aagggctgca
     1861 gggttcaggg tgggtctctt cacctctccc catcttgtga gctttaccga gaggatcagg
     1921 gttgacaatg tggagatatg cgaggcggaa gtggtggagc ttacccgcga gataaggaat
     1981 atcattctca acagtcagcc atcaccagcc gccattcagg acatggacca aacagtcgca
     2041 agcggtcagc agtcgttttt gcccaccttt tttgagttcg tgactgctct gggttttctc
     2101 tactttaagc ggaaagggat tgagtgggca gtggtggaaa caggaatggg gggaaggctg
     2161 gatgccacaa acattcttgt cccggcagtc tctgtgataa caaacatcag ttatgatcac
     2221 agggaattcc ttggtcagac cctgagagag atttcaggag aaaaggcagg gataataaaa
     2281 aaaggtgttc ctgtggtctc ttcagcccag gaggatgaag tgatcggggt aattacggaa
     2341 aaggccacag aattggggag cagtcttcat atctatggca aagacttctt ctcccatccc
     2401 aagaatattg atatacacgg gattacattc agctacgagg ggggagaaca aatggataat
     2461 ctccgcatct ctctcactgg attgcatcag gtggagaatg cgtcccttgc catcagggca
     2521 atagacgtgg gcatgaagaa aggggtcatt ccctcttatg cgataaggga agggctcgca
     2581 catgcccaat ggcctggcag gcttgaattg attgagaaca agggttgcac ctatgattac
     2641 ctcatcgatg gtgcgcacaa tccttcggca tcaagggctc tcgcaaattc cctgaaaaag
     2701 tatttcatcc cgctctatga gaaggttgtc ctcatcatgg gagtcatggc cgataaggac
     2761 atggaggcaa tgatgaagcc acttctgcct ctggcatcag aggtcatctt cactgcccct
     2821 gagtacgaaa gggctgcgtc ccctgtaagg ctcgcagaat atgcgaaagc actgggagtc
     2881 tcgccgagag taaaggattc tgtgaaagca tccatggaac tcgcccggga attagcgggg
     2941 aaaggccctc aaaaaacgct cataatgata accggttctt tttataccat tggcgaggca
     3001 aaaagcgccc tcggccatga gtgcagtgct cagtcgcttg cgaggctgcg gtgagcctgt
     3061 tcggacggtt tcagaatcca aaaatcaaaa tccaagatcc cggacatatt atctttatgc
     3121 cattcatttc tcattttgta ttcatttctt ttctgctatg tgtcgttccc tgctttggaa
     3181 atgagagcac tatgatagag tcagaaaccc tcgactatga tggggcgact tctacctata
     3241 cggcaaaagg ccacgtgagg atatacaggg acctgggaag catcgaggct gacgagatca
     3301 gctacaatga gcagaccggt gacgcctatc ccgagggcaa tgtcatatat gagaaccccg
     3361 atgtgaggat aaaggccaaa agg
//
LOCUS       DATKOU010000109        12198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4758794, whole genome shotgun sequence.
ACCESSION   DATKOU010000109 DATKOU010000000
VERSION     DATKOU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4758794"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VMU21_05010"
     CDS             <1..1393
                     /locus_tag="VMU21_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUO76920.1"
                     /translation="IGKIVYPQSVRAPFAPRVPSQILETSRAFSEIVSAVSPAVVNIS
                     TTKVVRRDTLPFSEDPFFDLFKPFHDFGLPKKWKEQSLGSGVIVSKDGYIITNNHVVE
                     QSEDIRVTLFDKRSFRGKIVGADPKTDIAVVKISADNLPTVPWGDSDKLQVGEFVLAI
                     GNPFGLSHTVTMGIISAVGRANVGIADYEDFIQTDAAINPGNSGGPLVNVKGELIGIN
                     TAIFSRSGGYQGIGFAVPSNMARLVMDQLMKEGKIVRGWLGVTIQDITPELSQKFGLK
                     DSKGALVGDISKGSPAEKSGMMRGDVILEFNGKEVKSVGSLRNIVSQSKVGSQVKLKI
                     LRGGKEYQLTVTVAELPKEVAGSPAEPSPEDIQKNAFSGITVMDLTREIARQLGLGAN
                     ERGVVVVKIEQGSSADEAGLKKGDVIQEIDKKKVAGLGDFNKITSSIESGDTTLLFIN
                     RGGRRFYITIKGS"
     gene            1450..2457
                     /locus_tag="VMU21_05015"
     CDS             1450..2457
                     /locus_tag="VMU21_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUO76921.1"
                     /translation="MLFFIRIAVLVVFISSGYFLGLKYNAFIEGTALGVVCGILALLM
                     EKAFKAVSLGKILGGLAGLLMGIIFANLLLLPLRRVAPDISVVSFVLNGLLGYGGMLL
                     GVKRGEKLTLSGLFRSFRGQGSDEHLKILDTSVIIDGRIADVCEAGFLDGVFLVPQFI
                     LQELQHIADSPDSLKRARGRRGLDILHRIQKMAHITVRITDEDFPKIKEVDSKLVALA
                     RVHDAKVITNDFNLNKVAELQGVSVLNINELANALKPVVLPGEALKVFVIKEGKEYNQ
                     GVAYLDDGTMVVVENGRRLIGKNAEVSVTSVLQTTAGRMIFSKPREEYEKEEFRAIKQ
                     T"
     gene            2463..3164
                     /gene="ispD"
                     /locus_tag="VMU21_05020"
     CDS             2463..3164
                     /gene="ispD"
                     /locus_tag="VMU21_05020"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HUO76922.1"
                     /translation="MKEKVIAIVPGAGLGKRFGPDTNKPFHVLFGKPLIVWTLEIFES
                     MNEVHEIIPVLKEPDMETGIKMFEVYGLSKVKRIAPGGKERQDSVYNALKLIKDSDDL
                     VLIHDGARPLIESRIVRTALQGISGFDGVVVGVPVKDTIKEVAGVVVKRTLKRETLWA
                     IQTPQIFIQRTLIKAYRAAMAEKFYSTDDAALLERSGGRVKIIMGSYSNIKVTTPEDI
                     PLAEHLLKERGKTGI"
     gene            3161..3637
                     /gene="ispF"
                     /locus_tag="VMU21_05025"
     CDS             3161..3637
                     /gene="ispF"
                     /locus_tag="VMU21_05025"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HUO76923.1"
                     /translation="MRIGVGYDSHRLVGGRRLIIGGVEIPFEKGLRGHSDGDVLCHAI
                     IDSLIGALGIGDIGRHFPDSDPIWKDASSVEMLKYVSELADASGFEVKWIDSVVIAER
                     PRLAPHIESMKKALSKAHILPGVINIKAKTDEGMGFVGRGEGIAAYAVCLLGKREM"
     gene            3816..5690
                     /gene="mnmG"
                     /locus_tag="VMU21_05030"
     CDS             3816..5690
                     /gene="mnmG"
                     /locus_tag="VMU21_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HUO76924.1"
                     /translation="MKKDFCYDVIVIGAGHAGCEAALASSRMGMSTCVFTMNLDTIAQ
                     MSCNPAIGGLAKGHLVREIDALGGEMAKNTDRTGIQFRMLNRSKGPAVWSLRAQADRV
                     LYRVSMRKTMESQTNLSIKQATIEKIVVEDGTVKGVLTTLGTFYRTEAVIVTTGTFLK
                     GLIHIGLEHFPSGRAGEFPSTGLSDSLRDLGLKMGRLKTGTPPRLDANTIDFSKTVPQ
                     PGDDPPPPFSYSTERITSPQLPCYITYTNSETHRVIRENLDRSPLYSGRIKSIGPRYC
                     PSVEDKVVRFADKERHQVFLEPEGIETNEYYANGISTSLPYDVQVRLVRTIPGLEEAE
                     IMRPAYAIEYDFVYPTQLMHSLETKLIQGLFLAGQINGTSGYEEAAAQGLMAGINATL
                     KIRGREPLVLGRHEAYTGVLIDDLVTKGTNEPYRMFTSRAEYRLLLRHDNADVRLMEK
                     GHRIGLLHESAYRRFQEERILMETEVSRLRATRLKPCMVNEGLAALHSSPIKEDSSLE
                     QLLKRPEITYEFIKRFSPPERPLPEDICREVEIQTKYHGYILRQKETAEKLKRMDEKK
                     IPESIDYTCISGLSREIVSKLEAVRPSNIGQASRIPGVTPAAISLIVIAVERYRRKAT
                     "
     gene            5691..6479
                     /gene="rsmG"
                     /locus_tag="VMU21_05035"
     CDS             5691..6479
                     /gene="rsmG"
                     /locus_tag="VMU21_05035"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HUO76925.1"
                     /translation="MRLSSSPSESLLIKGLTELSLPFAPAQVRAFMTYLSELKKWNKA
                     YNLTSLKTDEDIIVKHFLDSLLYLNALPNGKVSVMDVGSGAGFPGIPLKIMRPAIRMH
                     LLEPSRKKANFLRHITRTLTLDAIEVVEQRIEEVKSLTVDVAVTRALFEIKEFIGKAS
                     PHINEGGRLILSKGPKVKEELKGIKELHCEVLTLSLPTTHIKRFLIVIRKEFADLTPE
                     EIPPKSGAYHASTIFNITCVNSECRLRKAGCRGFEGCPGFKARD"
     gene            6838..7155
                     /locus_tag="VMU21_05040"
     CDS             6838..7155
                     /locus_tag="VMU21_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HUO76926.1"
                     /translation="MGKVLVAILMVALLASSAFAVMAGQVVEYKGGGAGKVVFDGSTH
                     ANKGLGCTDCHPSPFQMKKGSTEIKMEAVSRHKSCGACHDGTKAFKAEDKKNCAKCHQ
                     GSK"
     gene            7878..8090
                     /locus_tag="VMU21_05045"
     CDS             7878..8090
                     /locus_tag="VMU21_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76927.1"
                     /translation="MNTAFLTDKTYCIHLEKRIVARISRGLPMDKRPDLVLWVCHFPL
                     PSLSGIQCPVIVNGTQCPHNGPFGNN"
     gene            8266..9036
                     /locus_tag="VMU21_05050"
     CDS             8266..9036
                     /locus_tag="VMU21_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUO76928.1"
                     /translation="MAKTIAIVNQKGGVGKTTTAINLGASLALAEKDILLIDTDPQGN
                     STSGLGINREEVRRSLYDAYAEKCTPGEAVVQTSVQHLSLIPSVVDLLGVEVELVGRE
                     GRERILSRIIEGLEEKYKYILIDCPPSLGLLCLNALVAADSVIVPVQCEYYALEGLSL
                     LTRTLHRVRNSFNPSLEIRGILLTMFDTRNSLSHQVAEEVKHHFGGKVFDTVIPRNVS
                     LAEAPSHGKPIMLYDARSIGAQSYLSLAKEILSENGSR"
     gene            9017..9847
                     /locus_tag="VMU21_05055"
     CDS             9017..9847
                     /locus_tag="VMU21_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUO76929.1"
                     /translation="MKTALGKGLGSLLPDRAEDIIAIDIDRIIPGEQQPRKVFKDTAL
                     KELAASIKEKGVLQPIIVSRTGDGTFRLIAGERRWRAASMAGLRKMPAMVKDVSSVDS
                     LEIALIENIQREDLNPLEMAEAFHRLIKDFNLSQEELSVRVGKERATVANYLRLLKLP
                     DEIKTFVNDGTLSMGHARAILSVEGRPAQVETARRIIHKGLSVREAENLVKTWGAEGK
                     KRIRPKKRDPQIESLQEKLIHSLGTKVRILHKGKRGKIEIEYYSLDELERLLDILMPQ
                     "
     gene            9951..10169
                     /locus_tag="VMU21_05060"
     CDS             9951..10169
                     /locus_tag="VMU21_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HUO76930.1"
                     /translation="MKTVSLNVQKEFCEECSLALRRFIGHMDGVSSIDVEEGRIVIDF
                     DDGKIVEEEILKMTRESIEKLGYKTSDE"
     gene            complement(10472..11287)
                     /locus_tag="VMU21_05065"
     CDS             complement(10472..11287)
                     /locus_tag="VMU21_05065"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HUO76931.1"
                     /translation="MVVKASRKGAHRKRPRAPYVGMMLHQDGSRHEWVRGRFWDLMVT
                     MDDATGKHYSMFFVQEEGTESSFQGVREVIDSHGVFASLYTDRASHYWHTPSAGGKVD
                     KEHPTQFGAAMRQLCIEMIPAYSPEARGRSERAFGTHQQRLLKELALAGITDMDEANA
                     YIRTTYLRAYNEEFALAPEESASMFVPWAGAPIGDILCEKCERTVGNDNCVRFDGLAL
                     QIPPDRYRYHYVRVRVRVHRYPDRTLALFYGPRKLALYNSKGKEITACAQVAA"
     gene            complement(11281..11646)
                     /locus_tag="VMU21_05070"
     CDS             complement(11281..11646)
                     /locus_tag="VMU21_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUO76932.1"
                     /translation="MKRSEVLQEVRKMRFEEAYGGWKRGRLTQQEAAALLGVCERTFR
                     RYLVRYEEEGMLGLVDRRLGQLSHRRAPVDEVMAVAELYSRRYCGFAVKHCYSWYRRA
                     HGGTRSYTWVKNRLQDRGW"
     regulatory      11781..11857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11952..>12198
                     /locus_tag="VMU21_05075"
     CDS             11952..>12198
                     /locus_tag="VMU21_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76933.1"
                     /translation="MKQKISVVMMCVVAIMISMALPCLAQAAWIATDYQSYAQGDVVN
                     ITGFGWQPGESVSLSLYDVTDPNSPPQLWYTFINVYSP"
BASE COUNT         3311 a         2798 c         3234 g         2855 t
ORIGIN      
        1 catcgggaaa atcgtttatc ctcagtcggt tcgtgctccc tttgccccca gggttccctc
       61 ccagattctg gagacgagca gggcattttc cgagattgtg agtgccgtat cccctgcggt
      121 ggtgaatata tccaccacaa aagtggtgag gagagatacg ttgcccttct cggaggatcc
      181 tttttttgac ctcttcaaac cctttcacga tttcggtctt ccaaagaaat ggaaagaaca
      241 gagtcttgga tcgggagtca ttgtgtcaaa ggacgggtat atcattacca acaaccatgt
      301 ggttgaacaa tcggaagaca tcagggttac cctttttgac aagaggagct tcaggggaaa
      361 aatcgtgggt gctgatccaa aaaccgatat cgcagtagta aagatttcgg cggataatct
      421 ccctactgtc ccgtggggtg attcagacaa attgcaggtt ggtgagtttg ttcttgccat
      481 aggaaatccc tttggcctga gccacaccgt taccatgggt attatcagtg ctgtgggcag
      541 ggcgaatgtg ggaattgccg attatgagga ctttatccag acagatgctg cgatcaaccc
      601 cggcaattca ggaggtcccc ttgtaaatgt gaagggagaa ctcatcggca tcaatacggc
      661 gatcttctcg agaagcggcg gataccaggg cataggcttt gcggtcccga gcaatatggc
      721 ccgcctcgta atggaccagc ttatgaaaga ggggaagatt gtgaggggat ggcttggggt
      781 aacgattcag gatattaccc cggaactttc gcaaaaattt ggccttaagg actccaaggg
      841 ggcgcttgtg ggtgacatat caaaaggcag tcctgctgaa aaatcgggca tgatgagagg
      901 tgacgtcatt ctcgaattca acgggaagga agtcaaaagc gtgggaagcc ttcggaatat
      961 agtctcccag agcaaggtgg gttcccaagt gaaactaaag atactcaggg gtggcaagga
     1021 atatcaattg accgtcaccg tagccgaact tccgaaagaa gtggcagggt ctcccgcaga
     1081 gccttccccg gaagatattc aaaaaaatgc cttctccggt attaccgtta tggacctcac
     1141 cagggaaatt gcacgacaac tgggtcttgg cgccaatgag aggggggtag tggtagtcaa
     1201 aatagagcag ggaagcagtg cagacgaagc tggccttaag aagggcgacg tcatccagga
     1261 aatagacaag aaaaaagtgg cagggcttgg ggattttaac aagattacct catctattga
     1321 atcaggggat accactcttc tctttatcaa tagaggaggg aggagatttt atataaccat
     1381 taagggatct tagagatagt gaaacgcgac ggatatgtct agagagtgaa cgaagggagg
     1441 tgagaaagga tgctgttttt tattcgcata gcagtgttgg tggtgtttat ttcgtcaggt
     1501 tattttctgg gcttgaaata taatgcattc attgaaggga ctgccctcgg cgtggtatgc
     1561 ggaatccttg cacttttaat ggagaaggca ttcaaagccg tctccctcgg aaaaatcttg
     1621 ggagggctcg caggtctctt gatgggaatt atcttcgcta acctgcttct cctgcctttg
     1681 agaagggtgg ctcctgatat atcggtagtc tcgtttgtgc tgaatggact gttaggatac
     1741 ggcggtatgc ttttgggggt caagcggggg gaaaaactta ctctctcagg tcttttcaga
     1801 agcttcaggg gacagggttc agatgagcac ctcaaaatat tagatacgag tgtgataatt
     1861 gatggccgga ttgcggacgt gtgcgaggcg gggttccttg acggggtgtt cttggtgccc
     1921 cagtttatcc tgcaggagct tcagcatatt gctgattccc ctgattccct caaaagggca
     1981 cggggaaggc gggggcttga tattctccac aggatacaga agatggcgca catcaccgta
     2041 aggattactg acgaagattt tcccaagata aaagaggttg attcaaaact cgttgccctt
     2101 gcgagggttc atgatgcaaa ggtaatcact aatgatttta atctcaataa ggttgcggaa
     2161 cttcagggag taagcgtatt gaatatcaat gaacttgcca atgcgctcaa gcccgttgtt
     2221 cttcccggcg aggcattaaa ggtctttgtg atcaaagagg gcaaagaata caaccagggg
     2281 gtcgcatacc tggatgatgg gactatggtc gtggtagaga acgggaggag actcataggg
     2341 aaaaatgcag aggtctctgt aaccagtgtg cttcagacaa ctgcaggcag gatgatattc
     2401 tccaagccaa gggaagagta cgaaaaggaa gaattcaggg caataaagca gacctgacgg
     2461 ccatgaaaga aaaagtgatc gctattgtgc ccggtgccgg tctgggaaag agattcggcc
     2521 cggacaccaa taagccgttt catgtgcttt tcggtaaacc cctcatcgtc tggacgctgg
     2581 aaatattcga gtccatgaat gaggttcacg aaatcatccc tgtgttaaag gaacctgata
     2641 tggagacagg tattaagatg ttcgaagtgt atggactttc aaaggtaaag aggattgccc
     2701 cggggggaaa agaaaggcag gattctgtat acaatgcact taagctcata aaagacagtg
     2761 acgatcttgt tcttatccat gatggtgcac gccctctcat agaaagcagg attgtgagga
     2821 ctgcactcca gggtatctcc ggttttgacg gggtggttgt gggagttcct gtgaaggaca
     2881 ccattaaaga ggtggctggc gtcgtcgtga aaaggacgtt gaaacgtgaa acgctgtggg
     2941 caattcagac accccagata ttcatccaaa ggacactcat aaaggcttac cgtgcagcta
     3001 tggcggaaaa attctattcc acggacgatg ccgcactctt ggaaagaagc ggtggaaggg
     3061 tgaaaatcat tatgggttcg tattcaaaca taaaggtcac cacacctgaa gatatccctc
     3121 tcgcagagca cctcttgaaa gagcggggga agacgggaat atgagaatcg gcgtaggata
     3181 tgattctcac aggctcgtgg gaggcagaag gctcattatc gggggcgttg aaattccctt
     3241 tgaaaaaggc ctccggggcc attcagatgg tgatgtcctc tgccatgcca ttatcgactc
     3301 ccttattgga gccctgggga ttggtgacat cggaagacat ttccctgatt cggatccgat
     3361 atggaaagat gcgtccagtg tggagatgct caagtatgtc tctgagctcg ccgatgccag
     3421 cgggtttgag gtgaaatgga tcgactcggt ggttatcgcc gaaaggccca ggctcgcacc
     3481 acatattgaa tcgatgaaga aggctctctc aaaagcccat atccttccgg gtgtcataaa
     3541 tattaaggcc aagactgatg aaggcatggg ctttgtgggg cgcggagagg ggattgctgc
     3601 ttatgcggtc tgtcttctgg gaaaaagaga gatgtgagtc ctatagacat ggttttcacc
     3661 tggtgagact tttggaaaaa aataatatag ccgtttatct ctgaaataac gtatattcac
     3721 cgaatgttct atgaacttaa tttgaggctg ggcaaccaaa ccccgagtaa tttttccctc
     3781 aagagatacc gctcaatcgt tttgcagaag cgtttatgaa aaaagatttt tgctacgacg
     3841 tcatcgtgat cggggccggt catgcggggt gtgaggctgc actggcctcc tcccgcatgg
     3901 ggatgtccac atgcgtcttt accatgaatc ttgataccat tgcccaaatg tcctgcaatc
     3961 ctgccatcgg tggcctcgca aagggacacc tggttcggga gatcgacgcc ctcggtggcg
     4021 aaatggcaaa aaatacagac aggacgggaa tacaattcag gatgcttaac agatccaagg
     4081 gtcctgcagt gtggtcccta agggctcagg cggacagggt actttacaga gtttcgatga
     4141 ggaagaccat ggaatcccaa acaaacctct ccataaaaca ggcaacaatt gagaagattg
     4201 tagtggaaga cgggacggtc aagggagttt tgacaactct gggaaccttt tacagaacag
     4261 aagcagtcat cgtaaccaca ggcacgttct tgaagggact aatccatata ggtctcgaac
     4321 atttcccttc aggaagggca ggagagtttc cctccaccgg tctttccgat tccttaaggg
     4381 accttggctt gaaaatgggg cggctcaaga ctggaacgcc accaaggctg gatgcaaata
     4441 ccatcgattt ttcaaagaca gtgccccagc ccggcgatga cccaccaccg ccgttttcct
     4501 acagtaccga gaggattacg agtccacagc tcccctgtta tatcacctat acgaacagcg
     4561 agacccacag ggtcattcgc gaaaatcttg accgctcacc cctttacagc ggaagaataa
     4621 agtctatcgg tccgcggtac tgcccttctg tggaggataa ggtggtgaga ttcgctgata
     4681 aggagaggca ccaggtcttt ctcgaaccag agggcattga gacgaatgaa tattatgcaa
     4741 atggaatatc cacaagtctc ccctatgatg tgcaggtgag acttgtgagg accatcccgg
     4801 gacttgaaga agcggagatc atgcggcccg cctatgccat tgaatatgat tttgtgtatc
     4861 ccactcaatt aatgcacagc cttgagacaa agctgataca agggttgttt cttgcgggtc
     4921 agattaacgg gacctcaggg tatgaggagg ctgctgccca ggggctcatg gcagggatta
     4981 atgccacact caagatcagg ggcagagaac ccctggtcct cggcagacat gaggcgtaca
     5041 caggggttct tattgatgac ctggttacaa agggcaccaa tgagccctac aggatgttca
     5101 cctccagggc agaatacagg cttcttctga ggcacgacaa cgccgatgtc cgcttgatgg
     5161 aaaagggtca ccgcatcggc cttctccacg aatccgcata caggagattt caggaagaaa
     5221 gaatcctcat ggagactgaa gtatcgaggc tcagagctac aaggcttaag ccgtgcatgg
     5281 tgaatgaagg gttggccgcc ttacactcct cgcccataaa agaggattcc tcactggaac
     5341 aacttttaaa gaggccggag ataacctatg agttcataaa aaggttttca ccacctgaga
     5401 ggcccctacc agaagatatc tgcagagagg tggagatcca gacaaagtat catggttaca
     5461 tactcagaca gaaagagacg gcggagaagc tcaagagaat ggacgagaag aagatacctg
     5521 agtcaattga ttacacctgc attagtggcc tctcacggga gatcgtctct aaacttgaag
     5581 cggttagacc gtccaatata ggtcaggcga gcaggattcc cggagtcacc cccgccgcaa
     5641 tttccctcat agtcatcgcg gtcgaaagat atcgccgaaa ggctacgtaa atgcgccttt
     5701 catcttcgcc ttctgaatcg cttctcatta agggattgac agagctgtcg ttgccattcg
     5761 cgccggcgca ggtgagggct ttcatgacct acctctctga gctcaagaaa tggaataagg
     5821 catacaacct cacttccctc aaaacagatg aggatataat cgtaaaacac tttctcgact
     5881 ccctcctcta tctgaacgca ctgccgaacg gaaaggtgag cgtgatggat gtggggagcg
     5941 gcgcaggatt tccggggatc cccttaaaga tcatgaggcc cgcgatcagg atgcaccttt
     6001 tggagccatc gaggaaaaag gcgaactttc tgaggcacat tacccggaca cttacacttg
     6061 atgccattga agtggtggag cagcgaatcg aagaggtgaa atcactcaca gtggatgtgg
     6121 cagtcacaag agcgctcttt gagataaagg aattcatcgg gaaggcgtcg ccccatataa
     6181 atgagggagg aaggctcatt ctgagcaagg ggcccaaggt taaagaggaa ctcaagggga
     6241 taaaggagct gcactgcgag gtattgactc tatccttgcc gactacgcat atcaagcgtt
     6301 ttctcatagt catcaggaaa gaatttgcgg atctgacacc agaagaaatc cctccaaaat
     6361 ccggagcgta tcacgcatca actatcttca acatcacgtg cgtcaattct gaatgccgcc
     6421 tcagaaaagc gggatgcagg ggatttgaag gttgccctgg atttaaggcg agggactaat
     6481 gtcaggtgct tcgattcaca ccagattatc agggccggca tctctgataa cagggcggag
     6541 aataatggga cacagcgaat acggaaaggc ggcgactttt acccattcac tccccttgta
     6601 gcccttcata gtttttcata atcctttcgc tggacattca caattctttg ctaagctgta
     6661 accagcacaa agaaagagaa caacaagaaa ggaggagaga aaatgaaagt aaagggatcg
     6721 gcggggagaa ttcccgcttt ttgccaaagt gaaaaaaaga gtggatatac ggacaaaact
     6781 gtggtataaa tgataactgg ttgcaccatt tacttataag atatggaggg aatctgtatg
     6841 ggaaaggttt tggtagcaat actgatggta gctcttctcg caagcagtgc ctttgcggta
     6901 atggcagggc aggtcgttga gtacaaggga gggggagcgg gaaaggtcgt atttgatggc
     6961 agcacccatg cgaacaaggg ccttgggtgc actgactgcc acccctcgcc gtttcagatg
     7021 aaaaagggca gtacagagat caagatggag gccgttagca gacataagtc atgtggtgcc
     7081 tgccatgacg gaacaaaggc cttcaaggct gaagataaga agaattgtgc gaagtgccat
     7141 caaggctcta agtaagatgc ctgcggcata gttgggttgt atcggcctct tatgttccgt
     7201 gaggataagg aaaggcagcg ggtcttgagt aagcctgagt tattaaaggg taagccgtga
     7261 gtggagagag gtatttattt aaatgcagta aaaggaactt cacattgtga gggagagaag
     7321 aatacataca cgtaatccat tcgagcacga aataggatgt agttgcgctt ctgatcccgc
     7381 aaaggagata tttaaaagta ttactgttga catcagtgag catggactgg gtatttatgt
     7441 ctttcgaccc ctgaataaag gtcagaagat aatgatcaaa cgaggcctcc aatgcactca
     7501 caaaatagga tttgtcagat ggtgtgctgc atcaggtgaa aatgtgtaca gggcaggaat
     7561 ggtctttggc cgctcataat attgcctggc atttttctct gcggcatacg tccttttcat
     7621 aggcttcttc ttatgaagag ctcccgcacc caggacagac aagataatag atactgtttg
     7681 tccagtgctt atgattgttc caacaactgt ataactataa aaagatacat cctttattaa
     7741 tttatatagt tatttgtttc tttattttcc ccccttcctg atttttgttc cacgtggaac
     7801 acatattttg accttccttg ccctgcttcc gtattctctg ttagtctata agcagagaac
     7861 cgtgataaga ggtaaccatg aacaccgcat ttttgacaga caagacatac tgcatacatc
     7921 tcgaaaagag aatagtagca agaataagcc ggggccttcc tatggacaaa aggccagacc
     7981 tcgtcctctg ggtctgccat ttcccccttc cttccctttc gggcatacag tgccctgtca
     8041 tagtaaatgg aactcaatgc ccccataatg gcccctttgg caacaactaa gctttaaagt
     8101 tgtgtgaaaa gagtatgttc cacgtggaac atatgcacaa cagcgtagcg taaagagcaa
     8161 aaggaatcgg gcatgttagt ctcgaaccct tctgcactat gcccttagcc ccctgcatct
     8221 gtattgcctt gccctgtgaa cttcaactct gttagaatta ataaaatggc aaaaactatc
     8281 gccatcgtca atcagaaggg aggggtcgga aagaccacca ctgccattaa ccttggggca
     8341 tcactggccc tagctgagaa agatatcctc ttaatcgaca cagaccccca gggaaactct
     8401 acaagcggcc tcggcataaa ccgtgaagag gtgaggagaa gcctttatga tgcctacgca
     8461 gagaagtgca cccctggtga ggctgtggta cagacctctg tccaacacct ttcgctcatt
     8521 ccctcagtcg ttgaccttct aggggttgaa gtagaattgg tgggacgaga agggagagaa
     8581 cgcatccttt cgcgaataat cgaaggtttg gaagaaaaat ataagtatat tctcatagac
     8641 tgtccaccct ctctcggctt gctctgcctg aacgccctgg tagcagctga ctcggtaatc
     8701 gtaccggtcc agtgcgaata ttatgccctc gaggggctca gccttctcac aaggacgctc
     8761 catcgggtga gaaattcatt taatccttcc ctcgaaataa ggggcattct ccttacaatg
     8821 tttgatacaa ggaatagtct ttcccaccag gttgccgaag aggtgaaaca tcacttcgga
     8881 gggaaggtat tcgatactgt tatcccccgc aatgtatccc ttgccgaagc gccaagccac
     8941 ggcaagccca taatgctcta tgacgcccgt tccatcgggg cccaaagtta tctttccctg
     9001 gcaaaggaga tcctcagtga aaacggctct cggtaaaggt ctcggctctc tgctgcctga
     9061 cagggcagaa gatatcatcg ccatcgatat tgaccgaatc atcccgggtg aacagcaacc
     9121 gcgcaaggtc ttcaaagata cggcactcaa agagcttgcc gcgtccataa aggaaaaggg
     9181 tgtcttacag cccataattg tgtcgcgcac aggtgacggc acattcaggc tcatcgccgg
     9241 ggagaggaga tggagagccg catccatggc aggattaagg aagatgcctg ccatggtaaa
     9301 ggatgtctca tccgttgatt ccctcgaaat cgcgctaata gaaaatattc agcgtgagga
     9361 cctgaatccg ctggagatgg cggaagcctt ccatcggctt attaaggact ttaaccttag
     9421 ccaggaagaa ctctctgtca gggtcgggaa agaaagggca accgtcgcaa attacctgag
     9481 gcttctgaaa cttcctgacg agataaagac tttcgtaaat gatggtacat tgtcaatggg
     9541 tcatgccagg gctatcctgt cggtcgaggg aaggcctgct caagtcgaaa cagcaagacg
     9601 gataatccat aaaggattga gtgtcaggga ggcggagaat ctggttaaaa cgtggggggc
     9661 cgaaggaaaa aagaggatcc gtccaaagaa gagggacccc cagatagaat ctctccagga
     9721 aaaactaatc catagtcttg gcacaaaggt ccgcatcctt cataaaggaa agagggggaa
     9781 aatcgagata gaatactatt ccctggatga acttgagcgg cttctggata ttttgatgcc
     9841 gcaatgacca acaggaaagc gacaccgaac gaggaggaca ctcctgccgg cagacgctga
     9901 caggaatacg gctgtgcagt gagggagata acgcactccg gagattcatc atgaagaccg
     9961 tttctcttaa tgtccagaag gaattttgcg aagagtgttc tctcgcattg agaaggttta
    10021 tcgggcacat ggatggggtg agctcaattg atgtggaaga ggggagaatc gtaatagact
    10081 ttgacgatgg gaaaatcgta gaagaggaga tcctgaagat gaccagggaa agcatagaaa
    10141 aacttggcta caagacgagt gatgagtgat tggttgactc attaggagcc ttcggtaaga
    10201 aagatatatt gcctcaagat acactcgctt cgatcgtaaa ctcattgaaa atgtccctta
    10261 ttgtgcatgt tttttcggta gcatacatta tgagtcaacg atcccttttc gcttctccct
    10321 gcggcaacat cgtttacaag tcaattcatg aatgaggaaa cgccagaaaa aatctgttgt
    10381 caacaaatag agttgtccgg tttgggagca cataaactgt ccggttttct gtagaggggg
    10441 aacacatgaa acaaggttcc gcatattgtc gttacgcggc gacctgtgca cacgccgtga
    10501 tctccttacc ctttgaattg tacagtgcca gctttcgagg gccatagaac agggccagag
    10561 tacgatcggg atatcgatgg accctcactc tgaccctgac gtagtgatag cggtagcggt
    10621 caggagggat ttgaagggca agcccatcga acctgacaca gttgtcgttg cccacagtgc
    10681 gttcgcactt ttcacagagg atatccccaa tgggggcacc ggcccaaggg acgaacatgc
    10741 tggcagactc ctcaggggcc agggcaaact cttcattgta ggcccgcaga taggtggttc
    10801 tgatataggc attggcttcg tccatgtcgg tgatgccggc aagggcgagt tccttgagga
    10861 gccgctgttg gtgagtgccg aaggcgcgct ctgatcgtcc ccttgcctcg ggagaatagg
    10921 ccggtatcat ctcgatgcaa agctgtctca tggcagcgcc gaactgtgtg ggatgttcct
    10981 tgtccacctt tccgcctgca gagggggtat gccagtagtg gctagcacgg tccgtataca
    11041 acgaggcaaa taccccgtgg ctgtcaatga cctccctgac cccctgaaaa ctgctttcgg
    11101 tgccttcctc ctggacaaag aacatcgagt agtgtttgcc ggtggcatcg tccatggtaa
    11161 ccatgagatc ccagaacctt cctctcaccc actcgtgccg ggagccgtcc tgatgcagca
    11221 tcatgccgac atagggggcc cggggacgct tccggtgagc acccttgcgg gatgctttga
    11281 ctaccacccg cggtcctgca gccggttctt cacccaggtg tagctccgcg tgcccccatg
    11341 ggccctccgg taccaactgt aacaatgctt caccgcaaag ccgcagtagc ggcggctgta
    11401 caactccgca accgccatga cctcatctac cggagcacga cggtgcgaga gctgcccaag
    11461 acgacgatcc accagtccca gcatcccctc ttcctcgtat cggaccagat accgccgaaa
    11521 cgtccgctca cataccccaa gcagcgctgc cgcctcttgc tgcgtcagac gccctcgctt
    11581 ccatccccca taggcttcct caaaccgcat cttccgcacc tcctgtagta cctccgaacg
    11641 cttcatggat tgctcctcct tgcctatcca ttatcatccg gacagtttac gtgctcgcta
    11701 cacacgccag aaaaaaatgt tgacaaatag tgaattccat gatttactgt attagatttt
    11761 ttttaagaga aaatatgcac atcccgaaag gccaaaccct ctgggaaggg ggggcggaaa
    11821 gcaacgggtc tttaaaagag acagccgggc tgccgaaaga agcgcatggt tagcttggct
    11881 ttttttttga gcgaaataga ataatagcca gaacactgag gaaaaaatac gaaagggagg
    11941 ggttggttat aatgaagcaa aaaatcagcg tagtaatgat gtgcgtcgtt gccataatga
    12001 tctcgatggc attgccatgc ctcgcgcagg ctgcatggat agctacggat tatcagtcat
    12061 atgcgcaagg ggatgttgta aacattaccg gcttcggttg gcaaccggga gagtctgtct
    12121 cgttaagttt atacgatgta accgatccga acagcccacc tcagctgtgg tacacgttta
    12181 tcaacgtcta ttccccaa
//
LOCUS       DATKOU010000110         2468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4379018, whole genome shotgun sequence.
ACCESSION   DATKOU010000110 DATKOU010000000
VERSION     DATKOU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4379018"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..588)
                     /locus_tag="VMU21_05080"
     CDS             complement(169..588)
                     /locus_tag="VMU21_05080"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HUO76934.1"
                     /translation="MIHIFTRWLVKIDMVFHAFAAVLLLIACTFIFYSAVVNAAHPSR
                     DSILHLVNDVLLALIILELLWTVIRFLKKQHFVLGPFLAIGIIAAVRRILLIEAQTSL
                     VEHVPYEKLYEIGLSAVVIIILMISYYLSVKAEKLEQ"
     gene            713..1675
                     /locus_tag="VMU21_05085"
     CDS             713..1675
                     /locus_tag="VMU21_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76935.1"
                     /translation="MGETIFSNQGDAVYPRRPHYIQFYPTMRCNLSCSFCFNRGLSAT
                     PDITLNDFERLVATISGSGIRELDILGGEPTLHPEFEGMTDILYSHKIKVTISTNGYG
                     NLHVLEAVHKKSYDDLIKIGVSLNSAFVSEDLHEYLMKHRPMLKSVYTRKQGIPESAR
                     KYLAHPGTEYYLLYIDVVDKDSLVDSLPFYDFHRDLHALRKMHKNIKGVFCSGFIPDT
                     EGNAVLTHVRCPAGTTKLSVLPDGTAYPCYLLFRNREFALGNLLVDDFETIWNNPVLD
                     IFRQFKGNSCRETRCELHSACHGGCPALSLLISNDITAPDPRCR"
     gene            1863..>2468
                     /locus_tag="VMU21_05090"
     CDS             1863..>2468
                     /locus_tag="VMU21_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUO76936.1"
                     /translation="MQPKVLAFVLAGGKGERLFPLTAFRSKPSVPFGGTYRIVDFVLS
                     NLLNSHIYSIYLLVQYKSQSLIEHVRRNWVLSPVIKDHFVAVVPPQMRMGPEWFQGTA
                     DAVFQNMNLIREHHPGLVVVFGADHIYRMDIRQMIDFHLSKNAYVTVAARPVPIDQAS
                     SFGVIMTDRDKRIVGFQEKPKIPTPMADDPNRAYVSMGNYVF"
BASE COUNT          621 a          630 c          575 g          642 t
ORIGIN      
        1 attaatcctt tggtcttgtc aagttcaatg ctgctcttgc tccctttgaa atcaaagcgc
       61 tgggttatct cctgcatagc ctggtgttct gcattagaca cctcctgcat gtccacattg
      121 cacacaatgt caaaggaatg ctcatccgcc atcatatcct ccaattggtt actgttcgag
      181 tttttccgct tttacagaca ggtagtagga gatcatcagg atgatgatca ccaccgcact
      241 taggccgatt tcgtataatt tttcgtatgg tacatgttcc actaaagagg tctgcgcctc
      301 tatgaggagt atcctcctca ccgcggcaat gatgccgatg gcaaggaaag ggccaaggac
      361 aaaatgctgc ttcttgagga aacgtattac tgtccagagg agttcaagga tgatcaatgc
      421 caaaagcaca tcattcacaa ggtgcagtat cgagtcgcgg gaaggatggg cagcattgac
      481 caccgcgctg taaaaaatga aagtgcacgc gataagcaac agcactgctg caaaggcatg
      541 gaagaccata tctatcttca caagccatct ggtgaatatg tgtatcatgt ctttttttgc
      601 ctgctcttcc atggttttta aattatacgc ataccatgtt caaaaatcca tagcgaacag
      661 gccctcacaa gcgcctcttt cacctctggg cagttccctg gcatgatata atgtgggtga
      721 gacaatcttt tcaaaccagg gagatgcagt gtacccccgg cgaccgcact atatccagtt
      781 ctatcccaca atgagatgca acctctcatg cagcttctgc ttcaacaggg gcttgtcagc
      841 cacaccggat atcacgctta atgatttcga gaggctcgtt gcgaccattt ctggttcagg
      901 aatcagggaa ctggacatcc tgggaggaga accaaccctc catcccgaat tcgagggtat
      961 gacggatatc ctttactctc acaaaataaa agtcacgatt agtaccaatg ggtacgggaa
     1021 tctccatgtg ctggaggctg tccacaaaaa gagctatgac gacctgataa agataggggt
     1081 ctccctgaat tcggcttttg tatctgaaga ccttcacgaa tacctaatga agcatcgccc
     1141 catgctgaaa agtgtgtata cgagaaaaca agggattccg gaatcggcga ggaagtatct
     1201 cgcacacccc gggactgaat attatctgct ctacatcgac gtagtggata aagatagcct
     1261 ggtagatagt cttccctttt atgactttca cagagatctc catgcgctca gaaaaatgca
     1321 taagaacatt aaaggtgttt tttgctcagg tttcatccct gacaccgaag gaaacgcggt
     1381 attaacccat gtgaggtgcc ctgcgggaac caccaagctt tcggtcctgc ctgatggcac
     1441 tgcgtatccg tgttatctcc tcttcagaaa cagggagttt gccttgggta acctgttggt
     1501 tgacgatttt gagacaatat ggaacaatcc cgttctcgat atcttcagac agttcaaagg
     1561 caactcgtgt cgagaaactc gctgtgaact ccactctgcc tgccacgggg gatgtcctgc
     1621 gctcagcctc ctgatctcga atgatatcac cgcaccagac ccgcgctgta ggtagtggat
     1681 tagtcgtcgg ggatgaagcc cctgcgctcg cggtgccctg cattattcat cctgctccac
     1741 tttttcttga ccatcacctc ttctccatct atactgtagg taatatctga tgatagttag
     1801 tatgtttcgg acttctcgtt atccggtgca caggcgttca actctcttgt aaggaggcac
     1861 gcatgcaacc aaaggtcctt gcctttgtat tggcaggagg aaaaggtgaa cggctcttcc
     1921 ccctcaccgc ctttcgctcg aaaccctcag tgccatttgg cggaacttac aggatcgtgg
     1981 actttgtgct gagcaacctc ttaaattccc atatttattc catctatctc cttgtacagt
     2041 ataaatccca gtcactcatc gagcatgtga ggcgcaactg ggtactctct ccggtaatca
     2101 aggaccattt cgtggcagta gtcccacccc agatgcggat gggccctgaa tggttccagg
     2161 gaactgctga cgcagtgttt cagaatatga accttatcag ggagcaccat cccggcctcg
     2221 ttgtggtctt tggcgcagac cacatctaca ggatggatat acgtcagatg atcgattttc
     2281 acctctcgaa gaacgcctat gtaacagttg ccgcacggcc ggtccccata gatcaggcct
     2341 catccttcgg ggttattatg accgacaggg acaagaggat tgtggggttc caggaaaagc
     2401 cgaaaattcc gacgcccatg gccgatgacc cgaatcgagc ctatgtgtcg atggggaatt
     2461 acgtattc
//
LOCUS       DATKOU010000111         4120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2459023, whole genome shotgun sequence.
ACCESSION   DATKOU010000111 DATKOU010000000
VERSION     DATKOU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459023"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VMU21_05095"
     CDS             <1..293
                     /locus_tag="VMU21_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HUO76937.1"
                     /translation="GEADLSLLSAPEELRIIKKLLIYPMTFEGAAISREPHRITFFLQ
                     ELTGLFHPYYNKYKVVTEDRKLTRARLALCEAIRIVLKDGLEILGLGAPERM"
     gene            complement(309..2129)
                     /locus_tag="VMU21_05100"
     CDS             complement(309..2129)
                     /locus_tag="VMU21_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HUO76938.1"
                     /translation="MMKPNQGSALSGLVKAAGLVFGDIGTSPIYTMTVIFLLLKPTYD
                     NVLGVTSLIIWTLLVLVFLQYDFLAMSLSRRGEGGTVVLREILVSLLKSKKGTTLITL
                     LSFLGVSLLMGDGVITPAISILSAVEGSALIPGLEHISKTAIVLISAVIAIALFSFQS
                     KGTEKVAFGFGPIMAIWFISLSFFGIMSIIDSPHIIRALNPYYGIRFLVENGWKGYIV
                     LGEVILCATGGEALYADMGHLGSTPIRKAWLGVFFSLALNYLGQGAFLMNHPETKNVL
                     FEMAFHYMRDLYVPFLLLSIAATVIASQAMISGMFSIVYQGITTRIMPIFKVDYTSTE
                     RKSQIYISSVNWFLLFAVLFIMVEFEESSRLAAAYGLAVTGTMTLTGIMMTWIFTLKK
                     KPFLAILSLFVTFVDLSFLGANFYKIPHGGYWSLILASLPFGMILLYTQGQKRLYKTM
                     DFMPIEDFLRKFTMVYRTTPLIKGTALFLIKDIKEIPPYLVQIMFFHGIVYEDNIFVS
                     VLKRDDPFGITGFFREDIAPGLRVFEVQMGYMEVIDVEELLRQAGIEERTVFYGLEDI
                     TSENLIWKTFSLVKRITPTFIQFYKFPYKKLHGVLTKVEL"
     gene            2372..2653
                     /locus_tag="VMU21_05105"
     CDS             2372..2653
                     /locus_tag="VMU21_05105"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HUO76939.1"
                     /translation="MDKLYDEIARVAYELYEKRGKGEGFNLSDWLEAERIVMARHEKK
                     EKGEQGGVKPVKRKTTTRKPKEEKATAARKETPQKKTAGKRTATTKKTT"
     gene            2650..3660
                     /gene="amrS"
                     /locus_tag="VMU21_05110"
     CDS             2650..3660
                     /gene="amrS"
                     /locus_tag="VMU21_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HUO76940.1"
                     /translation="MKEARLYEKIGAGKVKCFLCAHYCTISPGKRGICAVRENRDGIL
                     YSLVYGKVIARHVDPIEKKPLFHFHPGSTSYSISTVGCNFKCMHCQNYDISQYPKQHP
                     DIPGEEMTPERVVREAELSGCKSISYTYTEPTIFFEFAYDCAKLAHEKGIKNIFVSNG
                     YTGPEATRLIAPYLDGNNIDLKGDDGFYKKVVGAKLQPVLDTIRLMKEMGIWMEVTTL
                     IIPTYNDSDEFLQWVASFIKSVDPAMPWHVTQFYPTYELQDVPRTPVSTLRMAREIGL
                     KTGLKYVYEGNVPGEGGENTWCPSCGELLIERFGFALSRIQMKNSRCSKCGAYIHGIG
                     MP"
     gene            complement(3765..>4120)
                     /locus_tag="VMU21_05115"
     CDS             complement(3765..>4120)
                     /locus_tag="VMU21_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HUO76941.1"
                     /translation="HNAQTAGKHIDKALYDKAKDYYEQAFYRVVFVGAENSVGFHNPT
                     ESLRILGDATAFAVKAEALLRQALAKAGVDTPLVVKLDLNKYLEGRGEKKIKFNQKFE
                     FKDPFGIEEQVSVGK"
BASE COUNT         1189 a          922 c         1035 g          974 t
ORIGIN      
        1 ttggcgaagc tgacctgagc ctcctgtctg cgcctgagga actcaggata atcaagaagc
       61 tgctcattta ccccatgacc tttgagggcg ctgcgatctc ccgtgagccc cacaggatta
      121 cctttttcct ccaggagctc acaggcctct ttcatcctta ttataataaa tacaaggtag
      181 tcactgagga cagaaaactg actagggcac gacttgcgct ctgcgaagct atacgtattg
      241 tgttaaagga tggccttgaa atcctgggcc tgggtgcgcc ggagaggatg taatgatgac
      301 agattttctt acaactcaac cttggttaag acaccatgga gctttttgta aggaaattta
      361 taaaactgga taaaggtcgg ggtaatcctc ttaacgagtg aaaatgtttt ccaaatgaga
      421 ttctcggacg taatatcttc tagcccgtaa aagacggtcc tctcctctat ccctgcctgt
      481 ctgaggagtt cctcaacatc aatgacctcc atgtatccca tctggacctc aaagaccctc
      541 agcccggggg caatatcttc acgaaagaag cctgtgatac caaaaggatc gtcccttttc
      601 aagaccgaaa caaagatatt gtcttcatac acaataccat ggaaaaacat tatctgaacc
      661 agataaggcg gtatttcttt tatatccttg ataagaaaga gagcagtacc cttgatcaga
      721 ggggtggtcc ggtataccat agtaaacttg cgaagaaagt cttcaatagg cataaagtcc
      781 atggtcttgt agagcctctt ttgcccttgg gtatagagaa gtatcatacc gaagggaagg
      841 gaggcaagga tcagcgacca atatccaccg tggggtattt tgtagaagtt cgcaccgagg
      901 aaactgagat caacaaaggt gacgaacaga gagaggattg cgagaaaagg tttcttcttc
      961 agggtgaata tccaggtcat cataatgccg gtaagggtca tggtgccggt caccgcgagt
     1021 ccatacgctg cggcaaggcg gcttgactct tcgaattcca ccatgataaa gaggacggca
     1081 aagagcaaaa accagttcac tgagctgatg tatatctggg actttcgttc tgtggaggta
     1141 taatcaacct tgaagatagg cataatgcgg gtcgtgattc cctgatacac aatggaaaac
     1201 attccgctta tcatggcctg agaagcgatc accgtggcgg caatgctcag gagcaaaaaa
     1261 gggacataga ggtccctcat ataatgaaat gccatttcaa aaagcacgtt ctttgtctcg
     1321 ggatggttca tcaaaaaggc accctgtccc aggtaattca gggcgaggga gaaaaatacc
     1381 cctaaccatg ccttcctgat aggagtggac ccgagatgtc ccatatccgc gtaaagagcc
     1441 tccccccctg tggcgcacag gataacctct ccgaggacga tgtatccttt ccaaccattt
     1501 tcaacaagga accgtattcc ataatacggg ttcaaggctc tgatgatgtg gggggaatct
     1561 atgatggaca tgattccaaa aaaagacaat gagataaacc agatggccat aattgggccg
     1621 aagccgaagg caaccttctc tgttcccttg ctttggaacg agaagagagc gattgcgatc
     1681 actgcagaaa tgagaactat ggcggttttc gatatatgtt caagccctgg tatcaaggct
     1741 gaaccctcga cagcactgag gatactgatg gcaggggtga tcaccccgtc acccatgaga
     1801 agggataccc caaggaaaga caggagagtg atgagggttg tccccttttt ggacttgaga
     1861 agggagacca ggatctctct aagaactacc gtgccaccct caccccttct gctgaggctc
     1921 atggcgagga aatcgtactg gagaaagacg agaaccaaca aagtccatat aatgagggag
     1981 gtgacgccta ggacattatc ataggtgggt ttgaggagaa ggaatattac ggtcattgtg
     2041 tatatggggc ttgtgccaat gtctccgaat acaagaccag cagccttgac aagccccgaa
     2101 agcgcacttc cctggttagg tttcatcatt caccggtgcg aacaaaaaag tacttttttt
     2161 tggctaaagt ttaccagata tatcacaggt tatgcataaa tagcaacaat aattacctcc
     2221 tcggaatggg gtaagtgcat tccctcattt tgctgcggcg ttttttatca gaagactgag
     2281 aagttccggc ttttgttgca cgcagatggg atatcatgat agcattaaga caaaagaatt
     2341 tgtcatttgt atcgacttgc gggaggtgtg tatggataag ttatacgatg agattgcaag
     2401 ggtagcctac gagctttacg aaaaaagggg aaaaggcgag ggttttaatc tttctgactg
     2461 gctggaagcg gaacgtattg tgatggcgcg gcatgaaaaa aaggagaaag gtgaacaagg
     2521 gggcgtgaaa ccggtcaaga gaaagacaac gacgaggaag ccaaaagagg aaaaggcaac
     2581 agccgcacgc aaggaaacac cccaaaagaa aacagcagga aagagaaccg ccacaacaaa
     2641 aaaaacaaca tgaaagaggc gaggctatac gaaaagatcg gtgcggggaa agtgaaatgt
     2701 ttcctctgcg ctcattactg caccatatca cccgggaaaa gaggaatctg tgcggtgagg
     2761 gaaaacaggg acgggattct ctacagcctg gtgtatggca aggtaatcgc aagacatgtg
     2821 gaccccatag agaaaaaacc cttgttccat tttcatccag gatcgacatc ctattccatt
     2881 tccaccgtgg ggtgtaactt caaatgcatg cactgccaga actacgacat atcccagtac
     2941 ccaaagcagc atcctgatat tcccggggaa gagatgaccc cggagcgtgt ggtcagagag
     3001 gcggaacttt cagggtgcaa aagtatatcg tatacgtata ccgaacccac catatttttt
     3061 gaatttgcgt acgactgtgc gaaacttgcc cacgaaaagg ggatcaagaa tatattcgtg
     3121 agcaatggat acaccggacc tgaagcaaca aggcttatcg caccctacct cgacgggaat
     3181 aacattgatc tcaaggggga tgatggtttt tacaagaagg ttgtgggagc aaagctccaa
     3241 cccgtactgg ataccataag actcatgaag gaaatgggaa tctggatgga ggtcacgacc
     3301 ctcattattc cgacctataa tgattctgat gaatttctgc aatgggttgc cagttttatc
     3361 aagtcggttg acccggccat gccttggcac gtaacgcagt tttatcccac ttatgaattg
     3421 caggacgtgc cgagaacccc tgtgagtact ctgcgaatgg cgagggaaat aggactgaaa
     3481 acgggcctga aatatgtgta tgaagggaat gtcccgggag aaggagggga gaacacctgg
     3541 tgcccctcat gcggggaact ccttattgag aggtttggat ttgccctgag cagaatccag
     3601 atgaagaact ccaggtgttc aaagtgtggt gcgtatattc atggaatcgg gatgccatga
     3661 agaattaagc gtctaagagt gacaagatta ttgtaatgat gcgtgtttca ccggtgggaa
     3721 ggatgcagta gctctcctcc ccaccgaatg atcagctgcc tttcttattt gcccactgaa
     3781 acctgttcct cgataccaaa gggatccttg aactcgaact tctgattgaa cttgatcttc
     3841 ttttctcctc ttccctctag gtacttgttg agatccagct ttaccacaag gggcgtatcc
     3901 actcccgcct tggcaagggc ctgtctgagg agcgcttctg cctttactgc aaaagccgtg
     3961 gcatcgccaa ggatcctcag ggactctgtg ggattgtgaa accccacaga attctcggcg
     4021 cctacgaata ccactcgata aaatgcctgt tcgtaataat cctttgcctt atcatacagt
     4081 gccttgtcga tatgtttacc tgcagtctgc gcattatgca
//
LOCUS       DATKOU010000112         5076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1307267, whole genome shotgun sequence.
ACCESSION   DATKOU010000112 DATKOU010000000
VERSION     DATKOU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307267"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..822)
                     /locus_tag="VMU21_05120"
     CDS             complement(151..822)
                     /locus_tag="VMU21_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HUO76942.1"
                     /translation="MVELNKIAIFSTLSASEVKEISPYVMPVSLKKKDVIFSEGDPSE
                     WLYIVAEGKVKITKLSQDGKEIILEVIHPMDFFGGLAVIRGFPYPANAIAMEDTRLIK
                     ISRSNLMRVLDRFPNLMYCMAQQVGDRMKESHETLKNIALERVEARIASLLLKLADKT
                     GKKGVEGVSIDMKLTKQDIAEMVGTTVETSIRTMSKFRKMGIVGEKEGKIVIKNLEKL
                     KSLWD"
     gene            complement(836..1324)
                     /gene="ilvN"
                     /locus_tag="VMU21_05125"
     CDS             complement(836..1324)
                     /gene="ilvN"
                     /locus_tag="VMU21_05125"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HUO76943.1"
                     /translation="MRHTISILVENKFGVLSRVSGLFSGRGYNIESLSVGETIDPQIS
                     LMTIVTTGDDQIIEQITKQLNKLIDVIKVTDMKELEHVEREMVLVKVAPREQDKTEVL
                     NLTEIFRGRIVDSSQKTYTVEITGDEKKIEAFIELMKPMGIKEFVRTGKVAIAREGVK
                     KK"
     gene            complement(1335..3065)
                     /gene="ilvB"
                     /locus_tag="VMU21_05130"
     CDS             complement(1335..3065)
                     /gene="ilvB"
                     /locus_tag="VMU21_05130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HUO76944.1"
                     /translation="MKISGAEIIIESLKREGVKHIFGYPGGVVLNIFDSLYENKDIQL
                     ILTRHEQGAIHAADGYARATGKAGVALVTSGPGATNTVTGIATASMDSIPLVVFSGQV
                     PTMLIGNDAFQEADIVGITRPCTKYNYLVKDVNDLSRIIKEAFHIATTGRPGPVLIDL
                     PKDVTSGKAEFTWPELDMRSYKPTYEGNKWMIAQAAHTIAKAKKPVIVAGGGVISSGA
                     YKELREFAEHSEIPVTMTLMGLGGFPGTHDLSLGMLGMHGTYYANKAVQESDLLIAIG
                     MRFDDRVTGKVDAFAPHAKIVHIDIDPTSIRKNIRVDVPIVGDVKRVLTVMNKVLKEE
                     VKEQWAEVRKAWLKQIEDWKAERPMTYTWSDELIKPQFVVEKIYELTKGDAIICTEVG
                     QNQMWAAQFYKYDKPRTWLSSGGLGTMGYGFPAAIGAQLAFPNKLVIDIAGDGSIQMN
                     IQELATAVINKLPVKVAILNNRYLGMVRQWQELFYNERYSYSHLDVVPDFVEIAHAYS
                     AVGLRATKPSEVEPVLKEAFKIHKPVFMDFVVNWKEKVYPMVPAGAPIDHMLFEEPEK
                     KAEKKLKAVK"
     gene            complement(3210..4862)
                     /gene="ilvD"
                     /locus_tag="VMU21_05135"
     CDS             complement(3210..4862)
                     /gene="ilvD"
                     /locus_tag="VMU21_05135"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HUO76945.1"
                     /translation="MRSGKIKKGLERVPHRALLYATGIPKTEMHKPFIGVATSFTDII
                     PGHIGMRDLERFIEKGVHTGGGWPFFFGVPGICDGIAMGHTGMHYSLPSRELIADMVE
                     TVAQAHQLDGLVLLTNCDKITPGMLMAAARINIPSIVITAGPMLSGHYKDRRLNLTSD
                     TFEAVGKYKKGLIKDEELEALEMCACPGAGSCQGMYTANTMACVTEALGMSLPKCATE
                     VAVSANKRRIAFESGRRIVDLVKKNITPRKIMTRDAFGNAITVDLALGGSTNTTLHIP
                     AIAHEAGIDLPIETFDIIGKKTPHIANMIPGGQHYMEDLDRAGGIPAVLKRLRDRLSN
                     TITVSGKGIFEIADSAVVFDEDVIRPLQKAYHQQGGIAVLRGNLAPGGAVVKQSAVSQ
                     NMMRFEGAAKVFDSEEQGMKAILAGKIRSGDVVVIRYEGPKGGPGMREMLSPTATIAG
                     MGLSESVALITDGRFSGGTRGPCIGHISPEAMEGGPIAVVKEGDRIRIDIPKRSIEVV
                     ISVDEMKRRLVQWKPPKPKISKGYLSRYARVVSSAGTGAVME"
BASE COUNT         1081 a         1450 c         1180 g         1365 t
ORIGIN      
        1 cacgttgttc cggagaaaga tagctgaaca taaagccctg tgggtcgttg atgcctctca
       61 tcatgcctcc tgcgatgatg tcgttcatca actatacctt atgcgtatat acattggaag
      121 tagtttttca gcagcctgct agagagcagt tcagtcccat agggacttga gtttctccag
      181 attctttatc acgatcttcc cctccttttc gcccacgatc cccattttcc tgaacttgga
      241 cattgtccgt atggaggtct cgaccgtggt acccaccatt tcggcaatat cctgcttggt
      301 cagtttcatg tcaatgctga ccccttctac tcccttcttc ccggttttgt ccgccagctt
      361 aaggagcagg gaggcgatgc gcgcctcaac cctttcgagc gctatgttct tgagtgtctc
      421 atgggattcc ttcattctgt ctcccacctg ctgcgccata cagtacatga ggttggggaa
      481 acggtcaagg acccgcatga ggttggaacg ggaaatcttt atgagccttg tgtcctccat
      541 ggcaatggcg tttgcgggat agggaaatcc ccggattaca gcaagccctc cgaagaaatc
      601 catgggatgt atcacctcaa ggatgatctc tttgccgtcc tgggagagct tggtgatctt
      661 caccttaccc tctgccacaa tatagagcca ctccgacgga tcaccctcgg aaaagatcac
      721 gtccttcttc ttgagcgata cgggcatgac ataaggggaa atttccttga cttcggaagc
      781 gctgagggta ctgaagatag cgatcttgtt gagttcaacc attatcgtac gagggttatt
      841 tctttttcac gccttccctg gcaatggcaa ctttccctgt tctcacgaac tccttgatcc
      901 ccattggttt catgagttcg ataaatgcct ctatcttctt ttcgtcaccg gtaatttcga
      961 cggtgtaggt cttctggctc gaatcaacga tcctcccgcg gaatatctcg gtgaggttca
     1021 ggacttcggt cttatcttgt tcccggggag ctaccttcac gagaaccatc tcccgctcca
     1081 catgctccag ttctttcatg tcggtgacct tgatcacatc aataagcttg ttgagctgtt
     1141 ttgtgatctg ctcaatgatc tggtcatccc ctgtggtcac tatggtcatg agagatatct
     1201 gggggtctat ggtctcgcct accgagaggc tttcgatatt gtagcccctt ccgctgaaga
     1261 ggcccgagac cctcgacagg acgccgaatt tgttctcgac cagaatggat atggtatgcc
     1321 tcatgggaat ctcctcattt gactgccttg agtttctttt cggccttttt ctccggttct
     1381 tcaaaaagca tgtggtcgat gggtgcacct gcaggaacca tgggatatac cttctccttc
     1441 cagttcacca caaagtccat gaacacaggc ttgtggatct tgaacgcctc tttgagtacc
     1501 ggctcaacct cggacggttt agtggcacgc aggcccacgg cgctgtatgc atgggcgatc
     1561 tctacgaaat ctggcacaac gtcgaggtgg ctgtatgaat acctttcatt atagaagagc
     1621 tcctgccact gcctcaccat tcccagatac ctgttattga ggatcgccac ctttaccggg
     1681 agcttattga tcaccgcagt ggccagctcc tggatgttca tctgaatgct cccgtctccc
     1741 gcgatatcga tcaccagttt gttcgggaag gcaagctggg caccgatggc agcagggaat
     1801 ccgtagccca tggtaccgag ccctccggag ctcaaccagg tccgaggctt gtcatattta
     1861 tagaactggg cagcccacat ctggttctgg cccacctcag tgcaaatgat ggcatcgccc
     1921 ttggtaagct catagatctt ttccacaaca aactgcggtt ttatcaactc atcgctccag
     1981 gtgtaggtca tgggcctttc ggccttccaa tcctcgatct gcttaagcca ggcctttctc
     2041 acctcagccc actgttcctt cacttcttct ttcaatacct tattcatcac cgtgagaacc
     2101 ctcttgacgt cgcccacaat gggcacatcc acccttatgt tctttctgat ggatgtgggg
     2161 tctatatcga tatgcacgat cttggcgtgg ggagcaaagg catctacctt tcccgtcacc
     2221 ctgtcgtcaa aacgcattcc aatggcgata agtagatcgg actcctggac cgccttgttt
     2281 gcatagtagg tcccgtgcat gccgagcatg cccagagaga ggtcatgggt accgggaaag
     2341 cctccaagac ccatgagggt catggttacg ggtatctctg aatgttctgc aaactctctc
     2401 agttccttgt aagccccgga tgaaattacg ccaccacccg caacaatcac cggcttcttg
     2461 gctttcgcta tagtgtgggc tgcctgggca atcatccact tgtttccctc ataggtgggc
     2521 ttgtaacttc tcatgtcaag ctcaggccag gtaaattcag ccttgccgga ggtcacgtct
     2581 ttgggaagat cgataagcac agggccgggt cgtccggtgg tggcaatatg aaaggcctct
     2641 ttgatgatcc tcgaaagatc gtttacatcc ttcacaaggt aattatattt cgtgcaaggc
     2701 cttgtgatgc cgacaatatc cgcctcctga aaggcatcat tcccgattag catggtcggc
     2761 acctggccgg agaataccac aagagggatc gagtccatag aggcagttgc gatgccggtc
     2821 acggtattgg tggcaccggg tcctgacgta acgagcgcta cccctgcctt ccctgtcgcc
     2881 cttgcatagc cgtcggctgc atgaatcgca ccctgttcat gccgcgtgag tatcaactga
     2941 atgtccttat tttcgtagag agagtcgaag atattcagga ccacgccgcc gggatagccg
     3001 aatatatgtt tcaccccttc ccgcttgagg ctctcgataa ttatctcagc accagagatc
     3061 ttcatagata atccactcct tcaaagttta accattgtaa caaaagaccg gacaaaatgg
     3121 aatactctac ccatactctt ccgctgcaag agatttgcag ggacttttga gaaatttctc
     3181 agcggttttc ggccgatcat gcatatgccc tattccatta ccgccccggt tcctgccgaa
     3241 ctcaccactc tcgcataacg tgagagatac cctttgctta tttttggctt tgggggtttc
     3301 cactggacaa gtctccgctt catctcatca acagaaatca ctacctctat gctcctcttg
     3361 gggatgtcaa tccttatccg gtcaccctct ttcaccactg cgataggtcc gccttccatg
     3421 gcctcggggg atatatgacc gatacatggc cccctcgtgc ctccggaaaa cctcccatcg
     3481 gtaataaggg caactgactc actcaggccc atcccggcaa tggtcgccgt gggggagagc
     3541 atctccctca ttcccggacc tccctttggt ccctcatagc gtatcaccac tacgtcccct
     3601 gaacggattt tccctgcgag gatcgccttc atgccttgtt cctcggaatc gaagaccttt
     3661 gccgctcctt caaacctcat catattctgg ctcaccgcgg actgcttcac cacagcgcct
     3721 cccggcgcaa ggttaccgcg gaggaccgca atgcccccct gctgatgata tgccttttgc
     3781 aaaggcctga tcacgtcttc atcaaagaca accgcagaat ccgcaatctc gaatatcccc
     3841 ttgccgctga cggtaatagt gttactgaga cggtccctga gcctcttcag tacggcaggg
     3901 attccgcctg cgcgatccag gtcttccata tagtgctgtc caccggggat catatttgcg
     3961 atatgggggg tttttttgcc gataatatca aaagtctcga taggaaggtc gatgcctgcc
     4021 tcatgggcaa tggcgggaat atggagggtg gtattcgtgg agccgcccag ggcaagatcc
     4081 accgtaatag cgttgccaaa ggcatccctc gtcataatct tcctcggcgt gatgttcttt
     4141 ttcacaagat ccacaatccg tctcccgctc tcaaaggcta tcctcctctt gttggcagag
     4201 accgctacct cagtagcgca cttcggaagg ctcataccca gggcttccgt gacacacgcc
     4261 atggtgttcg ccgtgtacat cccctgacag gaacccgctc cggggcaggc gcacatctcc
     4321 agggcctcca gttcttcatc ttttatcaat cccttcttat atttccccac agcctcaaag
     4381 gtatcgctgg ttaggttgag ccgtctgtcc ttgtagtgtc cggagagcat gggacccgcg
     4441 gtgattacaa tggatgggat gttgatcctc gccgctgcca taagcatgcc gggggtaatc
     4501 ttgtcacaat tcgtaaggag caccagcccg tccagctgat gggcctgggc aacggtttcg
     4561 accatgtctg caatgagttc ccgcgaaggc agggaatagt gcatgcccgt atgccccata
     4621 gcgatgccgt cacagatgcc gggaacaccg aaaaaaaagg gccagccgcc gcccgtatgg
     4681 acaccctttt cgataaatct ttcaagatcc ctcatgccga tatgtccggg gataatgtcg
     4741 gtaaaactcg tggccactcc gatgaacggt ttgtgcatct ctgtcttcgg aatacccgtg
     4801 gcgtataaca atgccctatg cggaacccgc tccagaccct ttttgatctt cccacttctc
     4861 acaataaaag ataccccctt cgttaaaatt aagaaatttt ttgacagggt taagtttaag
     4921 acataagagt ctcacactca gtcttagcct gtcctttact tccccgcagt tccttaattc
     4981 ttgtggctct tcatgtagtc cctgacaagc tccgccattc tgtctttctt gttcaatttt
     5041 agtttctgga gctttttccg ctcaatctct tcgtcc
//
LOCUS       DATKOU010000113        17652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5541887, whole genome shotgun sequence.
ACCESSION   DATKOU010000113 DATKOU010000000
VERSION     DATKOU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 17652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17652
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5541887"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VMU21_05140"
     CDS             complement(<1..656)
                     /locus_tag="VMU21_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76946.1"
                     /translation="MCERERAEEQSAHSIDKTDFTTSEPSGLCTCARVPLAKAVGMVL
                     AHDITEIRTNEFKGRAFKKGHVVRREDICHLQRLGKEHLFVLSVGDDEMHEDDAAYAL
                     ASLLIGRGVATGGEPREGKINIIAAKDGLLRIDRDILFHLNLLGEVMCATLHTNTIVR
                     RGQIVAGTRAIPLVVKKKVIDEAKRIAGSNGRTIEVKELRRPCVGIVITGNEVYHGRV
                     "
     gene            complement(669..1640)
                     /locus_tag="VMU21_05145"
     CDS             complement(669..1640)
                     /locus_tag="VMU21_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HUO76947.1"
                     /translation="MACSLNFETLLQESVKVHGHLCPGQVLGVKMSLLGMRKVGIQGP
                     RSRDKKNLLVFVELDRCATDAIQSVTGCSLGRRTMKFMDYGKMAATFVNLRTGKAVRV
                     SGREDAREKAKSVSNGGEHKYAAQIAAYKIMPEDELFDTMEVDVQLRPEDMPGRPMKR
                     IKCDLCREDIQDMREVYKEGKVVCRSCAGGGYYSVRRPFLFPAVVHKCHNDIEIKSKL
                     WIEVDGEPVFGRGRLLLLKEIIRHGSINRAAREVCISYRKAWSYIKAMEERLGIRLVE
                     RRAGGKNGGGATLTREAAEFLERYEQMEAGVREIVDERFRKVFGVKG"
     gene            complement(1642..1938)
                     /locus_tag="VMU21_05150"
     CDS             complement(1642..1938)
                     /locus_tag="VMU21_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HUO76948.1"
                     /translation="MSISGIVVKTAPEHLSNVLDNLKSSGLCDIFFHDEAGKIIVTVE
                     GKDTGEEVKKVKAILNMPHVLCANLAYSYCEEESGNTADHSKGVADAVPDVLKE"
     gene            complement(1935..2483)
                     /locus_tag="VMU21_05155"
     CDS             complement(1935..2483)
                     /locus_tag="VMU21_05155"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HUO76949.1"
                     /translation="MRRSGGKALIAVVTGAIILFTGSAAFADKKEKSEEDLGIRKESI
                     YEENKVSPKGGEYSRAEPGKSKKIERAFENSPPLIPHDLTGMLPIAETNNMCLGCHMP
                     DVAKGIGATPLPKSHFIDFETGKNLQGKLDGKRYNCMQCHVPQVNIPEPIKNAFKGEF
                     RHKKDKTQSNLLDNLNEGVKTE"
     gene            complement(2505..3299)
                     /gene="napH"
                     /locus_tag="VMU21_05160"
     CDS             complement(2505..3299)
                     /gene="napH"
                     /locus_tag="VMU21_05160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HUO76950.1"
                     /translation="MKYRFMVLRRVSQISLMALFITGNVLGWHILRGNLSTSRLFDLL
                     TLADPFAVLQVLAGGKRVSLQALLGALVIVFFFAIMGGRAFCSWVCPVNVVTDVANWL
                     RKRARLDTAPENSMNRKTRYWVLGMALVISALTGVAAFEWISPISMLHRGVIFGMGIG
                     WTVILGVFLFDLFIVKNGFCGHLCPLGGFYSLVGRMGVVKVRHDKKRCTLCGKCVEIC
                     PEAQVLPMVGKESGIVLSGECTNCGRCIEVCDEEAMKFGLRHVYGR"
     gene            complement(3314..4099)
                     /gene="napG"
                     /locus_tag="VMU21_05165"
     CDS             complement(3314..4099)
                     /gene="napG"
                     /locus_tag="VMU21_05165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HUO76951.1"
                     /translation="MPERREFILGVLKGLGAAGVGGIVWTGFLDGKKSYALILRPPGS
                     VTEGEFLVKCTKCGLCVEACPYKALILAKPGEGRPLGTPYFVMRENPCRMCRDIPCAV
                     ACPTGALDTNLVSEEDVSGKKILAVNRTKIGLAVIDRETCIAYWGIQCDACYRSCPVM
                     DKAITVEPVRNERTGKHAVMAPVVHSDHCTGCGLCEHACVTKKASIFILPRGIAMGEA
                     SARYIKGWEEKDEERLSELPEETITKTPRSEKNPLDYLNKGEF"
     gene            complement(4139..6910)
                     /gene="napA"
                     /locus_tag="VMU21_05170"
     CDS             complement(4139..6910)
                     /gene="napA"
                     /locus_tag="VMU21_05170"
                     /inference="COORDINATES: protein motif:HMM:NF010055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HUO76952.1"
                     /translation="MSLSRREFLKTAAAVAAATAAGISVPKEMEATAKQTEAGWKWDK
                     SVCRFCGTGCGIMVATKSDRIVAVKGDPVAPVNMGLNCIKGYFNAQIIYGADRLTEPL
                     LRVNEKGEFDKKGKFKPVPWKRAFDEMEKQFKKYYNEMGPTGVAMFGSGQHTIMEGYI
                     AAKLYKGGFRSNNLDPNARHCMASAVASFMQTFGIDEPAGNYDDMHHSDTIVLWGANM
                     SEMHPVLWSKITDRKLSDTQKVKVVNLSTFSNRCSTLADIEIIIKPNTDLALQNYILR
                     EIVYNRPEAIDWDFVNNNCIFSTGYVDIGYGLRPKIDHAKYRKSELDTASKEAYKVVT
                     KEEGIAMASLGIKAGEKMEMKHTNAADAHWAISFEDFKKGIEPYTLDFVALLAKGDSD
                     ESLEDFKKKLLKLADLYTEKGRKVVSFWTMGFNQHVRGTWVNEQIYAIHLMLGKQAQP
                     GNGAFSLTGQPSACGTAREVGTFAHRLPADMVVMNPKHRGVSEKIWKLPEGTLNPVIG
                     SHYVKIMRDLEDGKIKWVWVQVNNPWQNTANANHWITAAREMDNFIVVSEAYPGISAK
                     VADLILPAAMIFEKWGAYGNAERRTQHWKQQITPVGKAMSDSWQLMEFSKRFKLSDVW
                     KEQKIDEKTTLPNVLAKAKEMGYKESDTLFDVLFANKEAKGYKWPDPVGAGFSNTEAG
                     GDRRNVEGFKGYGFFVQKYIWEEYRKFGVGHGHDLAEFDAYHSVRGLRWPVVDGKETL
                     WRFNAEFDPYAKKADHGKFAFYGDAFKEIPKGDLLGPKGDEKVKLPNKAKIFLRHYIE
                     PAEIPDNEYPLWLCTGRVLEHWHSGTMTMRVPELYRAVPEALCFMSPKDAKKFGFKDN
                     ELVWVESRRGKVKARVETRGRNNPPQGLIFIPWFDENVFINKVTLDATCPISKETDFK
                     KCAVKVYKV"
     gene            7198..8940
                     /locus_tag="VMU21_05175"
     CDS             7198..8940
                     /locus_tag="VMU21_05175"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HUO76953.1"
                     /translation="MELSENAIKVLRARYLIKDEQGKNIETPDGMFRRVASTIASAEK
                     LYREDPYFWEERFYTLLSNLKFLPNSPTLMNAGKEIGQLAACFVLPVDDSMQSIFDTL
                     KNAALILQSGGGTGFSFSKLRPRTDVVRSTGGIASGPVSFMKIYNTATDVIKQGGARR
                     GANMGILRIDHPDILEFVRIKRDEREMTNFNISVAVTDAFMDALAHNGEYDLVNPRSK
                     AIAGRLKAKEVFDEIVASAWETGDPGLVFIDRINKYNPTPHIGIMESTNPCGEQPLLP
                     YEACVLGSINLSKYVPQLSTSGSRSSEKGIMNRINLKELSQDIKTAVRFLDNSIDVNR
                     YPLPDIEIMHKGNRKIGLGVMGWADMLIHLSVPYTRKSACGLARELMKFFRDTARQAS
                     VELAEKRGVFPNFKGSIYDAPGMPMVRNATTTTIAPTGTLSIIADCSSGIEPLFALAY
                     KRLILDTELYEMNKTFFRVAREKGFYTKGLEDTVIRKGSLKGIKEVPAEVKRLFKTAH
                     DIPPEDHIEMQAAFQEYTDNAVSKTINMPRRATKEDVARTYLLAYEKGLKGITIFRYG
                     TAKKGTLVKFTESD"
     gene            complement(9069..9145)
                     /locus_tag="VMU21_05180"
     tRNA            complement(9069..9145)
                     /locus_tag="VMU21_05180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9109..9111),aa:Pro,seq:ggg)
     gene            complement(9147..9530)
                     /locus_tag="VMU21_05185"
     CDS             complement(9147..9530)
                     /locus_tag="VMU21_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUO76954.1"
                     /translation="MHRSSAENIVKPQRPFPDKLFYKIGEVSRIAGVEPYVLRYWETE
                     FPFLRPRKNKSGQRVYIRKDLELVLQIKKLLYEDRFTIEGVRKKFGEPEPPKPIPSLP
                     AVQSSAHEVIQRVKKRLRDILSHLQ"
     gene            complement(9523..9807)
                     /locus_tag="VMU21_05190"
     CDS             complement(9523..9807)
                     /locus_tag="VMU21_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HUO76955.1"
                     /translation="MTKADLVDLIFGKVGLSKIESQNIIELIFDTVKQTLVEGESVKV
                     SGFGTFNVKKKNARRGRNPKTGEELQITPRRVITFRASNHFKDLIEKTNA"
     gene            9963..10760
                     /locus_tag="VMU21_05195"
     CDS             9963..10760
                     /locus_tag="VMU21_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76956.1"
                     /translation="MKKILLFLFLVNFVLIRHAYSLDNNYDVLGVQPVAPYGIFSTFS
                     ADSLKQGKVAIALGAGRAVDTDYWRFTGQIGYGITDRLELDVTVPYVLAWQDSIDGFQ
                     DIPLSLKYRFLDEGQYGPSIALLVGGSLHTGRSEFSTAGSIGGGIIISKRIGPVTGHA
                     NLLYFRPGSGPLTEEITLATGLDFAASNNFKILTELYGQKSYAGSWNRLELRLGYRFL
                     TAESFYTTVGAGTDLKNRNPEFRLFLSFSYLFPKERKEIKKIYEEEE"
     gene            10760..11146
                     /locus_tag="VMU21_05200"
     CDS             10760..11146
                     /locus_tag="VMU21_05200"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUO76957.1"
                     /translation="MFDLGIQELIVIFVVALIVFGPKRLPELGRTLGKGLFELKKAME
                     GIKDQINEEAGSTHTPMNTLPSQEEKKNEGNAQSDSAYAPPEGPPVNERREAEEDVPV
                     GSEGEKVDPATDKTGQERKEDTVNGR"
     gene            11136..11933
                     /gene="tatC"
                     /locus_tag="VMU21_05205"
     CDS             11136..11933
                     /gene="tatC"
                     /locus_tag="VMU21_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HUO76958.1"
                     /translation="MADERMPFTGHLSELRKRIIISLVALFAGFLASFNYSENIFDVL
                     TLPLRSEIGFSMRYPFISFIPTQIKNPTLVFLAPAEAFWMHLKVSFVVGLMLALPVIL
                     YQLWKFISPGLLAKEKKYIAPFVVTATLLFLAGAAFCFFFVLPFAMGFLLTYKIGSMT
                     PMLSVGNYVDFCLKFILAFGAVFELPIVIVFLTRMGIVTPKTLAKNRKYAVLLAFVAG
                     AILTPTPDAFNQTLMAVPIIILYEAGILVSRFFVPRKPVKEEGEAVP"
     gene            11942..12220
                     /locus_tag="VMU21_05210"
     CDS             11942..12220
                     /locus_tag="VMU21_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76959.1"
                     /translation="MSAIRGKSLKIITQDISEGYTTVNPLFLKPFDPETLKELYHRVM
                     KTQAEIRGERFPHNDTEAIRWRNLRLQRLHSAGMIIRTFARDKRIPLI"
     gene            12262..13902
                     /locus_tag="VMU21_05215"
     CDS             12262..13902
                     /locus_tag="VMU21_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO76960.1"
                     /translation="MEVSEDIKAAKDVIQAFLKAKKAVRMYPENNPIYKKTLEDTFSK
                     FTDLFSYRDELNLKIKQNEIFFDAEQVYANAEKEDNLALFFFKDGLRELAFMKGLSQP
                     EMEEFLKIISLDFDREAVDDDIVTLLWEKDFHYIKYVADDAFLVEDEDFERAEINQVK
                     SKAPAPDEILKAYAAAFESEDVKGISIVNLTDKDLQVLVKEMEKDTLDKTGKIYQILL
                     EMLLQAESVPEYEDVLSLLRNVFSYSLKQKNLRTAVDIMQQTRALAENPVFSDTIKRQ
                     MNLLLASVNSEESIKSLGEMFDSDAALDGQLVSEYTAFLNRTSISPFISLLGELESIH
                     GRKVVIDILITLGKHDIQAVAKGLQDNRWFVVRNIIYILRHIGDKKAVEYLLATAKHT
                     DERVRKEAIKTLGELKNALALQTLRDCLDDPDPSIRKIAVKALGSIGSETAKRIILEK
                     ASGKAFRDKEFDEKREFYEVLARWNQADVIDFLIGTLKRKALFGKAKVDENRACAAYC
                     LGLMGSKDSLTVLSKLRDSKNKLLREAVNTAIKKIEHV"
     gene            13895..15298
                     /locus_tag="VMU21_05220"
     CDS             13895..15298
                     /locus_tag="VMU21_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO76961.1"
                     /translation="MSEREDVEIARNAKDIINQLAVFIRNAHVHDPGNVAVVSAIEKF
                     ISTVNPLIGQGNTLTLELVGEFFYLNESRVRYSMEYLLNFDFLVREFKRRGLGSITLN
                     SQITLQDMQLFLKPFIASSFSQTPFDALSEGTAMIDSLSVGPLKKIKEEGDFDIRKQV
                     KKTYFNAVSFTQGVINKIKSGERVSIKKAKRVVENMVDMILEEEELLIGMTAIKDYDE
                     YTYHHSVNVSILSVALGQRIGLSRKALTELGLVALFHDIGKMEVPPEILNKPTNFDEN
                     EWKIVKKHPFWGVRAILKLKGLDPTSIKTAIVAFEHHLNYDMTGYPKLSKPLSLDFYS
                     RIVSLVDQYDAMTSARVYSRNPLAPDKSLSIMMERAGRELDPILFKFFINMIGVFPIG
                     TLVLLDTKELGLVYGSNTMFQDRPRVLVIIDSSGKRVKGDVVDLTEKESSGKYVRTIV
                     KTMDPNKYRINLAEYML"
     gene            complement(15317..16465)
                     /locus_tag="VMU21_05225"
     CDS             complement(15317..16465)
                     /locus_tag="VMU21_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO76962.1"
                     /translation="MKYFLSSGCALKWLEFPAVYRIPTDELYELDSAALEFLGRCAHR
                     EGCEAEGYDREFIDYAVQEGILTTHFTGETKPPLRKSPEPSLRYLELQITTRCNLRCR
                     HCYIGSSHLTELTMKTIRDTLDEFQELQGLRLLITGGEPLLHTKFKQINSLLPEYAFR
                     KVLFTNGVLLSEKLLKSLNVNEIQVSIDGIESGHDALRGKGTFKKAMAGLRKAAQAGH
                     DISVATMVHSMNLNEFEEMEELFKGMGIKDWSVDVPALEGNLRDNPSFRLPPEIAGEY
                     LRYGFWDGLHGGGEGFACGLHLAAVMADGEVAKCTFYRSSSVGNVDEGLEECWRRIVP
                     VKLEELACDCTRKKECRGGCRYRAGLFGNTLGKDIYRCVAYDVPLQRG"
     gene            16501..>17652
                     /locus_tag="VMU21_05230"
     CDS             16501..>17652
                     /locus_tag="VMU21_05230"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76963.1"
                     /translation="MVQFVMDFFDVIFPLNIGPLTYKGPTAYLGSIMPGMLVQAEIKG
                     SLHHGVIRGKTMHAPQGSIKEIHSILQDRPIFTTSIMSLVQWMAEYYLVSEGLVLKSM
                     LPKDIFETTTSREKVSAFGRSPVPLAPDLATDLGVVSTIKESISKKEYKTYLLQAPSL
                     MHEISYLSLIIKKARNIIILVPEVAHIEEVSPYLHELAGQRLTILHGRLPRAKRRTAF
                     HRVISGDSDVVLGTRLAVFAPLSSPSLIAVLQEHNRSYKNLEGLRYHARDIAVMRGYL
                     ERATVLLSSPCPSLESFHNSAIGKYSSLIPESMVQRPKIEVINMKTAKKMTPHLSRRT
                     MDATASCFKNKGNTLLLVNRKGYSILQCADCNSIEACPECSIPLVYHKDK"
BASE COUNT         4474 a         4694 c         3877 g         4607 t
ORIGIN      
        1 ttaaccctgc cgtggtaaac ctcgttaccg gtgattacaa tccccacaca gggtcttctt
       61 agctccttga cctcaatggt cctgccgttg gatcccgcaa tcctttttgc ctcgtcaatc
      121 acctttttct tcaccaccag tggtatcgcc ctagttcccg ccacaatctg ccctcttctc
      181 acgatagtat ttgtgtgaag cgtcgcacac atcacctctc caagcagatt aagatggaag
      241 aggatatctc ggtcaattct taaaagtccg tcctttgccg caattatgtt aatcttcccc
      301 tctctcggtt ctccccccgt tgccacgcct cgcccgataa gcaaagaagc gagcgcatat
      361 gctgcatcat cctcatgcat ctcatcatcc ccgactgaaa ggacaaacag gtgttccttt
      421 ccaagcctct ggaggtggca tatatcttcc cgtctcacca catggccttt cttaaaagcc
      481 cttcctttaa actcgttcgt ccttatttca gtgatgtcat gtgccaggac catgcccact
      541 gccttggcaa ggggaacacg tgcgcacgtg caaaggcctg aaggttcaga agtggtgaaa
      601 tcagttttgt ctatagagtg tgcgctttgc tcttctgctc tttccctttc acacatattc
      661 caatctcctc accctttcac gccaaacacc tttctgaatc tctcatcaac aatctccctt
      721 acccccgcct ccatttgctc ataccgttcc aggaattcgg ccgcttcgcg agtgagggtc
      781 gcaccaccgc cattttttcc tcctgcccgt ctctccacca acctgatgcc gagtctctct
      841 tccattgcct tgatataact ccatgccttt ctgtaagaga tgcagacctc cctggccgcc
      901 cggtttatgg agccatgcct gatgatctcc ttgagcaata gcagtcttcc ccttccaaac
      961 acaggctcac cgtccacctc tatccagagc tttgacttga tctctatatc gttatgacat
     1021 ttatgcacaa cggctgggaa taaaaaagga cgacgaacgg aataataccc tccaccggcg
     1081 catgacctgc acaccacctt tccctcttta tacacttctc tcatatcctg gatgtcctct
     1141 ctgcacagat cacacttaat ccttttcatc ggtctccccg gcatatcttc cggtctcagc
     1201 tgcacatcca cctccatcgt gtcaaagagc tcatcttcag gcattatctt atacgcagcg
     1261 atctgcgcag catacttgtg ctctcccccg ttactgacac tcttggcttt ttccctggca
     1321 tcttctcttc cgctcacccg tactgccttt cctgttctta ggtttacaaa agtagcggcc
     1381 atcttcccat aatccatgaa tttcatggtc ctgcgtccaa gactgcagcc ggtcaccgat
     1441 tgaatagcgt cagtggcgca cctgtccagt tcaacaaaga cgagaaggtt ctttttatcc
     1501 ctgctccgcg gaccttgaat ccccaccttt ctcataccca ggagcgacat cttcacgccc
     1561 agcacctgcc ctggacaaag atgtccgtga accttcaccg attcctgaag aagggtttca
     1621 aaattcaatg aacatgccat attattcttt taacacatcc gggactgcat cagcaacacc
     1681 ttttgagtgg tccgcggtat tccccgattc ttcttcgcag tatgaatacg caaggttcgc
     1741 gcaaagcaca tgcggcatat tgagaatcgc cttcaccttc ttcacctctt ctcctgtatc
     1801 tttcccctcc actgtcacga taatcttgcc cgcttcatca tggaagaaaa tatcgcagag
     1861 ccctgacgat ttcaggttgt ccaggacatt actcagatgt tcaggtgctg tcttgactac
     1921 gatgcccgat atactcattc tgtttttacc ccttcattca ggttgtccag caggttcgat
     1981 tgtgttttgt cttttttatg cctgaactca cccttaaagg cgttctttat gggctcgggg
     2041 atattcacct ggggcacatg acactgcatg cagttgtaac gcttcccatc aagctttccc
     2101 tgcagattct tccctgtttc gaaatcaatg aaatgggatt taggcagggg ggttgccccg
     2161 attcccttcg caacatccgg catatgacag ccaagacaca tgttattcgt ctctgcaatg
     2221 ggtagcatcc cggtaaggtc atgcgggata agaggagggc tgttttcaaa cgccctttcg
     2281 atcttcttgc ttttccccgg ctctgcacgg gagtattctc cacctttggg actaaccttg
     2341 ttctcctcgt atatgctctc tttcctgatt cccaggtctt cctcgctctt ttccttcttg
     2401 tctgcaaatg cggcgctgcc agtaaacagt ataatcgccc ctgtcaccac agcgatcaat
     2461 gcctttccac cgcttcttct catagtaacc ctcctcttct ttcactacct tccgtagaca
     2521 tgccttaacc cgaatttcat tgcctcctcg tcacatacct cgatgcatct cccgcaattg
     2581 gtacactctc cggaaagaac tattccgctc tctttcccca ccatgggaag cacctgcgct
     2641 tcagggcaga tctcaacgca tttcccgcac aaggtgcatc tttttttatc gtggcgtact
     2701 ttcaccacac ccattctgcc gaccagggaa taaaaacctc caagaggaca gagatgtccg
     2761 cagaagccgt tcttcacaat aaaaaggtca aaaaggaata caccaagtat cacggtccag
     2821 ccaataccca tgccgaagat aacccccctg tgaagcattg aaataggact tatccattca
     2881 aaggcagcaa cccctgtaag cgcagagatg acaagggcca ttcccaggac ccagtatcgc
     2941 gtctttctgt tcatgctatt ttccggggcg gtatcaagcc ttgccctctt tctcaaccag
     3001 ttcgcaacgt cggtcaccac atttacagga catacccagc tgcaaaaagc cctccccccc
     3061 ataatcgcga aaaaaaacac gatgacaagg gctccaagaa gcgcctgaag agagactctt
     3121 ttcccccctg caaggacctg gaggaccgca aagggatcgg cgagggtcaa taggtcaaag
     3181 agccttgaag tactgagatt gcccctcaga atgtgccagc caagcacatt ccctgtaatg
     3241 aaaagggcca taagggatat ctgggacacc cttctcagta ccataaatct gtatttcatt
     3301 accggataca cctttagaat tctcccttat tgagataatc caatggattc ttctctgagc
     3361 gcggggtttt ggttattgtc tcttcaggca attcgctcag gcgctcttcg tctttttcct
     3421 cccatccctt gatgtaccgt gcgcttgctt cacccattgc aatacccctg ggcagaataa
     3481 atatggaggc cttcttcgtc acacatgcgt gctcgcacaa cccgcatccc gtgcagtgat
     3541 cactatgcac aacgggcgcc attacggcgt gtttacccgt tctctcgttc cttacaggtt
     3601 cgaccgtaat cgccttgtcc atcaccggac atgagcggta acaggcatca cactggatgc
     3661 cccagtatgc gatgcaggtc tccctgtcta ttaccgccag cccgatcttt gtacgattca
     3721 ccgcgagtat cttctttccc gagacatcct cttcgctcac gagattggta tcaagggcgc
     3781 cggtggggca cgcaactgca caggggatgt ccctgcacat tcggcatgga ttctcgcgca
     3841 tcacaaaata gggagtcccc aacggtcttc cctcccccgg ttttgcaagg ataagtgcct
     3901 tgtacggaca cgcctcaacg cacagcccgc attttgtaca tttcaccagg aattcacctt
     3961 cagtgacaga gccaggaggc ctcaggatca acgcataaga cttcttcccg tcaagaaagc
     4021 ctgtccagac gattcctccg acacccgcgg cacccaggcc cttcaataca ccaagtatga
     4081 attccctccg ctcaggcatc ttagccctct ccccgctatc cataagaata gtaggtcctc
     4141 aaactttata caccttgacc gcgcatttct taaagtcggt ctccttggaa atgggacagg
     4201 ttgcatcaag ggtgaccttg ttgataaaga cattttcatc gaaccagggg atgaatatca
     4261 gaccctgtgg gggattattc ctccccctgg tctccaccct tgctttcact ttcccgcgcc
     4321 ttgactcaac ccaaaccaat tcattgtcct taaagccaaa cttctttgca tctttgggac
     4381 tcataaagca gagggcttcc ggaactgcac gatataattc agggaccctc atagtcatgg
     4441 taccgctgtg ccaatgctca agaacccttc cggtacagag ccacaacgga tattcattat
     4501 caggaatttc ggctggctca atataatgcc tgaggaatat ctttgccttg ttgggcagtt
     4561 tcaccttctc atctcctttg ggaccgagca ggtcgccctt ggggatctcc ttgaacgcat
     4621 ctccataaaa ggcgaatttg ccatgatcgg ccttctttgc atagggatcg aattcggcat
     4681 tgaacctcca gagtgtctcc ttgccatcaa ccacaggcca tctcaatccc ctcacgctgt
     4741 gataggcatc aaactctgcg agatcatggc catgaccgac gccgaacttt ctgtactcct
     4801 cccatatata tttctgtaca aaaaagccat accccttaaa cccttccacg tttcttctat
     4861 cccctccggc ctccgtgtta gagaaacctg cacccacggg gtcaggccat ttatatccct
     4921 tcgcttcctt gttggcaaag aggacatcaa agagggtatc gctttcctta tagcccatct
     4981 cctttgcctt cgcaaggaca ttgggcaggg tagttttttc atcgatcttc tgctccttcc
     5041 agacatccga gagcttgaac ctcttggaga actccatcaa ctgccaggag tcagacattg
     5101 ccttgcctac aggagtgatc tgctgtttcc agtgttgtgt cctcctttcc gcatttccat
     5161 acgcacccca cttctcgaat atcattgctg cgggaaggat aaggtcagcc acttttgcgg
     5221 atatgccggg atatgcctca gagacgacaa tgaaattgtc catctcacgc gcagcagtaa
     5281 tccagtgatt cgcatttgcg gtattctgcc aggggttatt cacctggacc catacccatt
     5341 tgatctttcc gtcttccagg tcccgcatga tcttcacgta atgggaacct atcaccggat
     5401 tgagggtgcc ttcgggaagt ttccatatct tctcgcttac acccctgtgc tttgggttca
     5461 tcacgaccat gtcagcaggg agcctgtgtg cgaaggtccc cacttctcgt gcggttccac
     5521 atgcactggg ctgtcctgtc aggctgaagg ccccgtttcc tggctgtgcc tgttttccga
     5581 gcatgagatg gatagcgtat atctgttcat tcacccaggt accccgcacg tgctggttaa
     5641 agcccatggt ccagaagctc accaccttcc tgcccttttc agtatagaga tccgccagct
     5701 ttagcagttt cttcttgaag tcttcaaggg attcgtcact atctcccttt gcaaggagtg
     5761 ccacaaaatc cagggtgtaa ggctcaatgc cctttttgaa gtcctcgaag ctgatggccc
     5821 agtgggcatc tgcggcattg gtatgcttca tttccatttt ttctccagcc ttgataccca
     5881 aagaggccat cgcaataccc tcttccttcg tcactacctt atatgcttct ttactcgcgg
     5941 tatccagttc tgacttcctg tattttgcgt ggtcgatctt cggtcgtaat ccatagccga
     6001 tatcgacata tcctgtggag aaaatgcagt tattattcac aaaatcccag tcaatcgctt
     6061 ctggcctgtt gtacacgatt tccctcagaa tgtagttctg cagcgcaaga tcggtgttcg
     6121 gctttatgat aatctctatg tcagcgaggg tcgaacatct gttcgagaag gtgcttagat
     6181 tcaccacctt aactttctgg gtatcactca gtttcctgtc agtgattttt gaccagagaa
     6241 cgggatgcat ctctgacatg ttggcacccc agagcacaat agtatctgaa tggtgcatgt
     6301 catcatagtt tccggctggt tcatcaatac cgaaggtctg cataaaggaa gctaccgcac
     6361 tggccatgca gtgcctggcg ttgggatcca ggttgttgga cctgaaccct cctttgtaga
     6421 gttttgcagc aatatatccc tccattatcg tatgctgccc tgacccgaac atggcaaccc
     6481 cggtggggcc catttcgttg tagtatttct tgaactgctt ttccatctca tcaaaggccc
     6541 gcttccaggg gaccggcttg aacttacctt tcttgtcaaa ttcgcctttt tcattcactc
     6601 tcaggagggg ctctgtgagg cggtcggcac cgtagatgat ctgtgcatta aaatagcctt
     6661 taatgcagtt gaggcccata ttcactggag caaccgggtc accttttacc gcaacaattc
     6721 tgtcactctt tgttgccacc atgatgccac atccggttcc gcagaacctg cagaccgact
     6781 tgtcccactt ccatcctgcc tcggtctgtt ttgccgttgc ttccatctcc ttaggcacac
     6841 ttattcccgc agcggttgca gctgctaccg ctgcagccgt cttaagaaat tctcttctgg
     6901 aaagtgacat ggctaccccc tcctctttct tctcaacttt ttataagacc gcaccatttt
     6961 ttgcacttac ccatagacac agaaataata ctaaggtatg aactcataaa ctcttatgac
     7021 ctatatcatg ccagctcaca atggcaaaaa gggaaagaga gaaacagact ccatgtaatg
     7081 tcattctaac caaagtctat atccaaagta cctgcctgtc aagcgaggga aatcagcgcc
     7141 cttcaccctt ttcaggccac ccattgtgaa tgctcgatgt tttgatatac ttaaaaaatg
     7201 gagctctccg aaaatgccat caaagttctg agggcacggt accttattaa agatgaacag
     7261 ggaaaaaaca tcgaaacccc tgacggcatg ttcagacgtg tcgcttccac catcgcttca
     7321 gccgaaaaac tctatcgtga agacccttac ttttgggaag agcggtttta tacccttctc
     7381 tccaatctca aattcctccc caactccccc accctcatga atgccggcaa ggaaatagga
     7441 cagttggctg catgctttgt ccttccggtg gacgattcca tgcagagcat ctttgatacc
     7501 ctgaaaaacg ccgctcttat tctgcagagt ggagggggaa caggcttttc gttctcaaag
     7561 ttacggccga ggacagatgt ggtgcgttcc acaggcggca ttgcgagcgg tcccgtttcg
     7621 tttatgaaga tttacaacac cgcaactgat gtcatcaagc agggaggggc aagacggggc
     7681 gcaaacatgg ggatcctgag gattgaccac cccgacatcc ttgaatttgt cagaatcaaa
     7741 agggatgaaa gggagatgac aaattttaat atctcggtgg ccgtcacgga tgccttcatg
     7801 gatgcccttg cgcacaacgg cgaatacgat ttagtgaatc cgaggagcaa ggcaatagcg
     7861 ggaagattaa aggcaaaaga ggtatttgac gagattgtgg caagcgcatg ggagacaggg
     7921 gatccgggtc tggtttttat tgacaggatt aacaagtaca accccacacc acatataggg
     7981 attatggaaa gcacgaaccc ttgcggtgaa cagccattgc tcccctatga agcctgtgtc
     8041 ctcggttcca ttaatctttc caagtatgtc ccccaactct ccacttctgg ttcgcggtca
     8101 tctgaaaaag gcattatgaa ccggattaac cttaaagaac tgtcacagga catcaagact
     8161 gcagtgaggt tcctcgataa ttccatagat gtgaacaggt atccccttcc tgacatagaa
     8221 atcatgcata aagggaacag aaaaataggt cttggtgtta tgggctgggc tgatatgctc
     8281 atacatctta gtgttcccta taccagaaag agtgcctgcg ggcttgcaag agagcttatg
     8341 aaattcttca gagataccgc caggcaggcg tctgtggaac tggctgaaaa aagaggggtt
     8401 ttccccaatt tcaagggttc gatttatgac gcgccgggaa tgcccatggt gagaaatgcc
     8461 acgacgacga ctattgctcc cacagggact ctctccatca ttgctgactg ctccagcggc
     8521 atagagccgc tgttcgccct cgcatacaag cggcttatcc ttgatacaga gctttacgag
     8581 atgaacaaga cttttttccg tgtcgcgaga gagaagggtt tttataccaa gggactagaa
     8641 gacacggtaa tcagaaaagg ctcattaaag ggaatcaaag aggtacctgc tgaggtaaaa
     8701 cgcctcttca agacagccca tgatatcccg cctgaggatc atatcgaaat gcaggcagca
     8761 ttccaggagt acaccgataa tgcggtttcc aagaccatca acatgcccag gcgggccaca
     8821 aaagaagatg tggcaaggac ctatcttctc gcgtatgaaa agggattaaa ggggataacc
     8881 attttccggt atggcacagc aaagaaagga accctcgtga aattcaccga gtcggattga
     8941 acatgcttaa aagggacata tcctcttcac ttaccgcact tcaaatgcat atagactcag
     9001 caggacgaat ctgaaaaaat ctctgcacta gtaagattca ggaaatccaa ataaatcgtt
     9061 gattaaattg gtcggggcga cccgatttga acgggcgacc actcgcaccc caagcgagtg
     9121 cgctaccagg ctgcgctacg ccccgattat tggaggtggc tcagtatgtc cctgagcctt
     9181 tttttgaccc tctgaatgac ttcgtgtgca gaggactgga cagcagggag agatggtatt
     9241 ggcttaggcg gttcgggctc tccaaacttc tttctcaccc cttctatggt aaacctatcc
     9301 tcatagagca gcttcttaat ctggagtacc agttccaggt cctttcttat atataccctc
     9361 tgtcccgact tattctttct cggccgtaaa aaggggaact ccgtctccca gtatctcagg
     9421 acatagggct cgacgccggc aatcctgctc acttccccga tcttataaaa taacttgtcc
     9481 gggaaaggtc tttgaggctt aacgatattt tctgctgacg acctatgcat tcgtcttttc
     9541 tatgaggtct ttgaaatggt tgctcgcccg gaaggtgatg acccttcgcg gtgttatctg
     9601 aagctcctct cctgtcttgg ggtttctccc ccgcctcgca ttcttttttt tcacattgaa
     9661 ggtaccgaag ccggagacct ttaccgattc accctcaaca agggtctgtt ttacggtatc
     9721 aaaaataagc tcaatgatat tttgtgactc tattttcgat aatcccacct ttccaaatat
     9781 aagatcaacc aggtcagcct tcgtcatata cacctcactt cagatcatta taaagtagta
     9841 aaccgcccag acagacagtt ttcattgctc tgattttaga ttatagtgag gagatagacg
     9901 attgtcaaga gaaatcaatc acttattgac actagttaat tttttctgtt atctttaggc
     9961 tgatgaaaaa aattctcctt ttcctcttcc ttgtcaattt cgtccttata cggcatgcat
    10021 atagtctcga taataattat gatgtgctag gtgtccagcc tgtggcgcct tatggcatat
    10081 tttcgacctt cagtgcagac agccttaagc aggggaaagt ggctatcgct ttgggagcgg
    10141 ggagggcggt ggacactgat tactggcggt tcaccggcca gattgggtac ggaataaccg
    10201 acaggcttga gcttgatgtt accgtgccct atgttctcgc gtggcaagat tctattgacg
    10261 gatttcagga tataccgctc agtttaaaat acaggtttct tgacgagggg caatacggcc
    10321 catccatcgc gctccttgtg ggcgggtcct tgcatacagg aagaagtgaa ttcagtactg
    10381 cagggagtat cggcggagga attatcatct caaaacggat aggacctgtg accggacatg
    10441 caaatctgct gtatttcagg ccgggaagcg ggcctttgac ggaagagatt actcttgcta
    10501 ccgggcttga tttcgcagct tcgaacaact tcaaaatact gactgaattg tacggccaaa
    10561 aaagttacgc gggcagctgg aacagacttg agctgcgctt aggatacagg tttcttaccg
    10621 cagagagttt ttataccacg gtcggcgcag gcactgacct gaaaaacagg aaccccgaat
    10681 tccgtctatt tttgtccttc agctatctct ttccaaaaga gagaaaagag atcaagaaga
    10741 tttatgagga ggaggaatag tgtttgattt agggatacag gaactcatag tgatctttgt
    10801 agttgccctt atcgtcttcg gacccaaacg gctgcccgaa ttggggagga ccctgggtaa
    10861 gggactcttc gaactcaaga aggctatgga aggcatcaag gaccagatta acgaggaagc
    10921 cgggagcacc catactccca tgaatactct cccttcacag gaagagaaaa aaaatgaggg
    10981 gaatgcgcaa tctgattcgg catacgcacc acccgagggc cctcctgtca atgaaagacg
    11041 agaagccgaa gaggatgttc ccgttggttc cgagggggaa aaagttgacc ctgctactga
    11101 taagacagga caggagagaa aggaagatac ggtaaatggc agatgagaga atgcctttca
    11161 caggacacct ctccgaactt cggaaaagaa tcatcatctc cctcgtagcc ctttttgccg
    11221 ggtttctcgc ctcattcaac tattctgaaa atatcttcga cgtgcttacc ctccccctca
    11281 ggtcagagat agggttcagc atgaggtatc cctttatctc gttcattcct actcaaataa
    11341 aaaatccgac tcttgtattc cttgctcctg cagaggcatt ctggatgcat ctgaaggtct
    11401 cctttgtggt agggctcatg cttgccctgc ccgtcattct ctatcagtta tggaagttta
    11461 tttctcctgg attgcttgca aaggaaaaaa agtatatcgc tcccttcgta gtcaccgcaa
    11521 cgcttctttt tcttgcaggt gctgcctttt gcttcttctt cgtcctcccc tttgccatgg
    11581 gttttctcct gacgtacaaa atcgggtcca tgacacccat gctgtctgtg ggtaattatg
    11641 ttgacttctg cctgaaattc atccttgcct ttggtgctgt ttttgaactt cccatcgtca
    11701 tcgtcttctt gacacgcatg ggaatcgtga cacccaaaac cctcgcaaag aacagaaagt
    11761 atgccgtact gcttgcattt gtggccggtg cgatactcac tccaactcct gacgccttta
    11821 accagacatt gatggcagta cctatcatca ttctctatga agcaggcatt ctggtatcaa
    11881 gatttttcgt tccacgcaaa cccgttaagg aggaggggga agcagtcccc taacgcattg
    11941 gatgtctgcc atcagaggaa aaagcctgaa gattatcacc caggatatat ccgaggggta
    12001 tactacggta aatcccctgt ttttgaaacc ctttgatccc gaaaccctca aggaactcta
    12061 tcaccgcgta atgaagaccc aggcagaaat ccgtggtgaa cgatttcccc acaatgatac
    12121 tgaagccatc cgatggagga acctgaggct ccagagactc cacagcgcag ggatgattat
    12181 taggaccttt gccagggata aacgcattcc cctcatttga atccaatttg acacctatgg
    12241 aaatctatgt tattttttga catggaagtt tccgaagaca taaaagctgc caaggatgtt
    12301 atacaggcat tcctgaaggc aaaaaaggcc gtaaggatgt atcctgaaaa caatcccatc
    12361 tacaagaaaa cactcgaaga taccttctcg aagtttaccg acctcttcag ttaccgggac
    12421 gagcttaatc tgaagattaa gcagaatgaa atcttttttg atgccgagca ggtctatgca
    12481 aatgccgaaa aggaagataa cctcgctctc tttttcttca aagacggtct gagggagctg
    12541 gcgttcatga aagggctgtc tcagccggag atggaggagt tcctcaagat catttctctt
    12601 gactttgacc gagaggcagt tgacgacgac attgtcacgc tcttatggga aaaagatttt
    12661 cactatataa aatatgttgc cgatgacgca ttccttgtgg aggatgaaga ttttgagagg
    12721 gcggaaataa accaggtaaa aagcaaagct ccggcacctg atgagatact taaggcgtat
    12781 gccgccgcat tcgaatccga ggatgtcaag ggaatttcca tcgtgaacct taccgacaag
    12841 gatctgcagg ttcttgtgaa agagatggaa aaagatacac tggataagac aggcaagata
    12901 taccagatac tcctggagat gctcttacag gcagagagtg ttccggaata cgaggatgtc
    12961 ctcagcctcc tcaggaacgt attttcttat agtctcaaac aaaaaaacct aagaaccgct
    13021 gtcgatatca tgcaacagac aagggcgttg gcggaaaatc ctgttttctc tgacaccatt
    13081 aagcgccaga tgaacctcct gctcgcatcc gtcaactctg aagaatcgat aaagagcctg
    13141 ggtgagatgt ttgattcgga cgcggccctt gatggacagt tagtgagtga atatacggcg
    13201 tttcttaaca gaacctctat ttctcccttt atttcacttc tcggggaact ggagagcatt
    13261 cacggaagaa aggtcgtcat tgacatactt attaccctgg gcaaacatga catccaggct
    13321 gttgcaaagg gacttcaaga caaccgctgg tttgtggtaa ggaacattat ttatatcctt
    13381 cgccatatag gggataaaaa ggcggtggaa tatctgctcg ccactgcaaa acatactgac
    13441 gaacgggtgc ggaaagaggc gataaagacc ctgggagaac tcaagaacgc tctggcattg
    13501 cagaccttga gagactgcct tgatgacccg gacccgtcca tacggaagat tgcggtaaag
    13561 gcattagggt ccataggttc cgagaccgca aaacgcataa tccttgaaaa agcctctgga
    13621 aaagcgttca gagacaagga gtttgatgaa aaaagggagt tctatgaggt cttggcgcgc
    13681 tggaatcagg ctgatgtaat cgacttttta ataggcacac tgaaaaggaa ggcgcttttc
    13741 ggaaaagcta aagttgatga gaacagggca tgtgctgcat actgccttgg attaatgggc
    13801 agcaaagact cccttaccgt cctttccaag ctcagggatt cgaagaacaa actattaaga
    13861 gaagcggtaa atactgccat aaagaagata gaacatgtct gagagagaag atgtagagat
    13921 cgcgagaaac gcgaaggata tcatcaatca gcttgccgtg ttcatcagaa atgcacatgt
    13981 gcatgatccc gggaatgtcg cggtggtctc tgccattgag aagtttatct ccacggtaaa
    14041 tcccttgatc ggtcagggca atacgctcac cctcgaactc gtgggagaat tcttttatct
    14101 gaacgagagc agggtcaggt actccatgga atatctcctc aatttcgatt tcctcgtaag
    14161 ggaattcaag agaaggggtc tcgggagcat cacgctgaac agccaaatca cgctgcagga
    14221 tatgcagctc tttttaaagc cattcattgc ttcatccttt tcccaaactc ccttcgatgc
    14281 cctttcagag ggcactgcga tgatcgattc actgagcgtc ggacccctca agaagattaa
    14341 agaagagggg gattttgaca taaggaaaca ggtcaaaaag acatatttta acgcggtttc
    14401 gttcacacag ggggtcatca acaagatcaa gtcaggggaa cgggtgagca tcaagaaggc
    14461 aaaaagggtc gtagagaaca tggtggacat gatactggaa gaggaagaac ttctcatcgg
    14521 catgaccgcc attaaagatt atgacgagta tacgtatcac cactcggtga atgtgagcat
    14581 cctttctgta gccctgggcc agaggatcgg cctcagcaga aaggctctca ctgagctcgg
    14641 tctggttgcc ctcttccacg acatagggaa aatggaagtt ccccctgaaa tattgaacaa
    14701 acccaccaat tttgatgaaa acgaatggaa gatagtcaag aagcatcctt tctggggcgt
    14761 gagggcgatc ctgaagctca aaggcctgga tcccacttcc atcaaaactg ccattgtcgc
    14821 cttcgaacac cacctgaact acgatatgac agggtatcct aagttatcca aacctctttc
    14881 actcgatttt tattccagaa ttgtgagcct tgtggaccag tatgatgcca tgacctcagc
    14941 gagggtctat tcaaggaatc ccttggcgcc tgacaagtca ctgagtatta tgatggagag
    15001 ggcgggaagg gaactggatc cgattctctt taaattcttt attaatatga tcggcgtgtt
    15061 ccccatcggc acccttgtcc tcttagatac caaagagctt ggactcgtat acggaagtaa
    15121 taccatgttc caggacagac ccagggtctt agtcattata gacagcagcg gcaaacgcgt
    15181 caaaggagat gtagtagatc tcacggaaaa agagagcagc ggcaagtatg tgagaaccat
    15241 cgtaaagacc atggatccca acaaatacag gatcaatctc gcagaatata tgctctaact
    15301 aatatcttcg aaatccttac cctctctgca acggaacatc atatgcaacg caccggtaaa
    15361 tatctttccc aagggtattg ccgaacagtc ctgccctgta tctgcaacca cccctgcatt
    15421 ccttctttcg cgtacagtca caagcaagct cctccagctt caccgggacg atccttctcc
    15481 agcactcttc cagcccctca tccacatttc ccactgagga agaccgatag aatgtgcatt
    15541 ttgccacctc tccgtcagcc atcactgctg caagatggag accgcacgca aatccctcac
    15601 ctcccccgtg aagtccatcc caaaaaccat acctcagata ctctcccgca atttcaggag
    15661 gcaatctgaa cgatgggtta tccctgagat ttccctcaag agcggggaca tcaacagacc
    15721 agtccttgat tcccatccct ttaaaaagct cttccatttc ttcaaattca ttcaggttca
    15781 tggaatgtac cattgtggcc acagaaatat catgtcctgc ctgtgcagcc tttcggagcc
    15841 cggccatcgc ctttttgaat gtaccttttc ccctcaacgc atcgtgccct gattcaattc
    15901 cgtcgatact tacctggatt tcattaacat taagggattt cagtaatttc tcagaaagca
    15961 ataccccgtt ggtaaatagt accttcctga acgcgtactc aggaaggaga ctgttaatct
    16021 gcttgaattt agtatggaga agaggctcgc cccctgtaat caagagccgt agtccctgta
    16081 gttcctggaa ttcatccagg gtgtccctta tcgtcttcat ggtgagttca gtcaggtggg
    16141 atgacccaat ataacagtgc ctgcacctga ggttacatct tgtagttatc tggagctcca
    16201 ggtatctcag ggagggctcg ggagattttc ttaagggtgg cttagtttcc ccagtgaagt
    16261 gggtggtgag gataccttct tgcaccgcgt aatcaataaa ttccctgtca tatccctccg
    16321 cctcgcaacc ttctcgatgt gcgcatctcc ctaaaaactc aagggcagcg gaatcaagct
    16381 catagagttc atccgtgggt atgcgataga cggccgggaa ctcaagccac ttcaatgcgc
    16441 atcctgaaga aagaaaatac ttcatttttg tattatacct cacagcccag gcagcttttc
    16501 atggtacaat tcgtcatgga ttttttcgat gtaatctttc ccctcaatat cggtcccctc
    16561 acctataagg gtcccaccgc atacctcgga tctattatgc ctggcatgct tgtgcaggcg
    16621 gaaataaaag gatccctgca ccacggggtt atacggggga aaaccatgca tgcccctcag
    16681 ggttccataa aagagataca ctccatcctc caagacagac cgatcttcac cacctccata
    16741 atgagcctcg tacaatggat ggcagagtat tacctcgtga gcgagggtct tgtgctcaaa
    16801 agtatgctgc caaaggacat ttttgaaacc acaacctcaa gggagaaggt atcggcattc
    16861 ggacgcagtc cggtgccact cgctcccgat ctcgcaacag atcttggggt cgtatccacc
    16921 ataaaagaat ccatctcaaa aaaagaatac aaaacgtacc tccttcaagc cccttcgctt
    16981 atgcatgaga tctcctatct ctcactgatt atcaagaaag caagaaatat cattatcctt
    17041 gtcccggaag ttgcgcatat tgaggaggtg tcgccctatc tccatgaact cgcagggcaa
    17101 aggctcacga ttctccatgg aagattaccg cgcgccaaga gaagaactgc ttttcaccgg
    17161 gtaatctccg gagattctga tgtggtcctg ggcacgagac tggccgtatt cgccccattg
    17221 agctctccct ctcttatcgc cgtattgcag gagcataaca ggtcttacaa gaaccttgag
    17281 ggtctcagat atcatgcccg ggacatcgcc gtgatgcgcg ggtatctgga aagagcgaca
    17341 gtgctcctct cttccccatg cccgtccctg gaatctttcc ataactccgc aatcggaaag
    17401 tattcttccc tcatccctga aagcatggta caaaggccaa aaatagaggt catcaatatg
    17461 aaaactgcaa aaaaaatgac cccccatctc tccaggagaa cgatggatgc tactgcctcc
    17521 tgctttaaga ataaggggaa cacccttctc ctcgtaaaca gaaagggcta ctccatactc
    17581 caatgtgctg actgcaactc catcgaggcc tgtccggaat gcagtatccc ccttgtgtac
    17641 cataaagaca aa
//
LOCUS       DATKOU010000114         7985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1690243, whole genome shotgun sequence.
ACCESSION   DATKOU010000114 DATKOU010000000
VERSION     DATKOU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690243"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /gene="ftsH"
                     /locus_tag="VMU21_05235"
     CDS             complement(<1..1659)
                     /gene="ftsH"
                     /locus_tag="VMU21_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUO76964.1"
                     /translation="MRRIKALNSVYRSLFIWLMIGLIMILLFNLVSTPKKMEDEVIFS
                     DFMTKVKAGEVEDVVIKENLITGRLKDGKKFRTYSAGYPELVNELMTKGVKIAAKPPE
                     QNPWYVNFFFSWGPIIFLALVWIFFMRQMQTGGNKALSFGKSRARLVSDKSVKITFAD
                     VAGIEEAKAEVQEIIDFLKDPQKFSKLGGKIPKGVLLVGSPGSGKTLLAKAIAGEAGV
                     PFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPCIVFIDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFEGTEGVILLAATNRPDVLDPALLRPGRFDRQVVVPIPDVKGR
                     LEILKVHTRKIPLDSDVNLELIARGTPGFSGADLANLINEAALFAARKSKSKVDMPEF
                     EAAKDKVLMGVERKSMIISEEEKKNTAYHEAGHALVAKLTPGTDPIHKVSIIPRGRAL
                     GVTQQLPLDDRYTYSKDYLLKTLNVLLGGRAAEELVLHHMTTGAGNDLERATDLARRM
                     VTEWGMSEKLGPLTFGKKDEQIFLGREIAKHKDYSEKIAEEIDDE"
     gene            complement(1714..2454)
                     /gene="rph"
                     /locus_tag="VMU21_05240"
     CDS             complement(1714..2454)
                     /gene="rph"
                     /locus_tag="VMU21_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HUO76965.1"
                     /translation="MRPDGRENGQMREVKVTRNFLKTAEGSVLFEMGDTKVICSASVE
                     ERVPLFLRDQKKGWVTAEYGMLPRSTQTRMTRESVTGKIGGRTHEIQRLIGRTLRAVI
                     DLESLGERTVWVDCDVIQADGGTRTASITGAFICVADALRSAMKNGIIDKMPLTNHLA
                     AVSVGIVGGEPRLDLCYAEDSAADVDMNVVMTGDGRFVEIQGTSEKAPFSMDGLGMLL
                     ALADGGIKRLIEVQKEHLGNESFLNFHK"
     gene            complement(2473..3276)
                     /gene="murI"
                     /locus_tag="VMU21_05245"
     CDS             complement(2473..3276)
                     /gene="murI"
                     /locus_tag="VMU21_05245"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HUO76966.1"
                     /translation="MNNRPIGVFDSGIGGLTVLKEVFRKLPRESTVYLGDTARVPYGI
                     RSAETVTRYSFENTGFLVSMGIKVLVVACNTVSAVSLEEIKGSLSIPVIGVIEPGSRA
                     AVRATRNKNVGVIGTEATVNSGAYARIIRAMDASIRVFSLPCPLFVPLAEEGLTDDAI
                     ATMVAERYLKNLREKGIDTLVLGCTHYPLLKDVIGKVMGGGISLIDSATETASEIKET
                     LVRNGIEGNPSREPLREFYVTDAPERFRKVGERFLGEKIAHIEKISVGQ"
     gene            complement(3280..3846)
                     /locus_tag="VMU21_05250"
     CDS             complement(3280..3846)
                     /locus_tag="VMU21_05250"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HUO76967.1"
                     /translation="MSINKRRILWVFVFILLFSGGIAGGFFYFAGNFGMKPKGEAPSE
                     PQKQADDSSFIRVYFPSEGRLIIEERRVKRLGSVTVSAAEVVEEFLKGPSNREKSDVP
                     PGTKMLGIYFGNDGILYVDLSDEFRRNFHGDALSEFLLLKGLYESIISNVTGVDDVKI
                     LIEEKEMESIGGHLYALYPLKNTLTELR"
     gene            complement(3833..4819)
                     /locus_tag="VMU21_05255"
     CDS             complement(3833..4819)
                     /locus_tag="VMU21_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUO76968.1"
                     /translation="MAAKVFLIVLGISLFMTDAPMFESVYAIEGKTEVSMRFSTHEGV
                     RRVVFEAPDESFVRNTVVTSTQNQIKVQFPSNLALTVQGSPDMDASLKGRTYVINLSY
                     PFKTKLLQLTSPPRLSIDIVQLTKEESSQPGAKEGSSLPLIPNIRIVIDPGHGGYDLG
                     ILSGELREKEATLSLARAMESSLSKRNRTAFLTRKADQFLSLTERAMFANQKSADIFI
                     SIHLTPSQSFAVYSSLAESAAFDTMNEYSIMVRQRRFIEKSKALAEGIGKAIKDEFKK
                     DVIFRKMELPLLSSVGAAAVMIEIPAATISDQAGKARVLDALFRGIALYVNQ"
     gene            4987..5796
                     /locus_tag="VMU21_05260"
     CDS             4987..5796
                     /locus_tag="VMU21_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76969.1"
                     /translation="MNTHKSRFMFFISVAAGVLLFFSQSAFSSESTITILGDVVGNGD
                     TYMKAAFKQDIPLRGKPYPLIDGAHLRAGDGMMSVVFRDGAKLEVGKFSDLVVNGSRG
                     SYIVNMGSGKIGFVIPQGTSFSVKTPNSTIQTQSAAPAIQKASLSSQSTTVKGVVSYD
                     GKGTKVMAVSGTLTVRSGIGVVLQTVSEGNAIYIDGKDGGRIRTAQMATTPPPSPPPP
                     PPPPTPPSPPPPPPSGTAGEDAIVAGSVAGAGTGAYILTRQEYSVGVTPKE"
     gene            5937..6974
                     /locus_tag="VMU21_05265"
     CDS             5937..6974
                     /locus_tag="VMU21_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUO76970.1"
                     /translation="MKKLVVVAAVLALFSFVLIGCGGGSQPVQVQSLDSEIKQEGNST
                     RMNDLLLKAAMARPSNRDADYIIGPDDVLDIDVFQAEELHRTVRVSYQGYIGMPLIGQ
                     IKASGLTPLQLEQLIAQKLEKYMQEPRASVAIKEYKSQKISVIGAVGKSDVYSVTGQK
                     YLVDMLIMAGGISGGASEICLILRPSDSEMAGEKKTETLAINLIELLEKGDFSLNIPV
                     FSGDIINVLKNGIVYVDGPVNRPGPYGFQMKMTLREAIIIAGGLRFEADSSDVKILRD
                     KGDGTREVIAVNYDDITQGKTNDFPIKENDIIIMGKNGFKNFLVTVSGMLSGGLNTTG
                     GFVVNPATPIK"
     gene            7073..>7985
                     /locus_tag="VMU21_05270"
     CDS             7073..>7985
                     /locus_tag="VMU21_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HUO76971.1"
                     /translation="MNNFDNAPQVNTSLQKADEYPVAFPANYSFEQEREIHLRDYLRV
                     ILRRKWIVITFFLISVTTVILGTFMTKPMYRSSVTLKIEKDSPQVLNIKEAYEVDKSS
                     DAYYQTQYKILRSRNLAKRVIRAMKLDQNPVFIGYVPTPKAVTNKSIDTRSNTDMDED
                     FSPAVVDRLIAGLGIEPQDKSRIVKISFDSQSPELAASVPNAVAKSYIDYNIESKFDA
                     TLQTRDWLEKQLEDMKAKVERAEETLNRYVAQNGIIFLADSGSGDTKESKGQSMVNKK
                     LSDLSNQLVQSTSDRVTKEVMFREAQRE"
BASE COUNT         2037 a         2098 c         1831 g         2019 t
ORIGIN      
        1 ctcgtcatcg atctcctcgg caatcttttc gctgtagtct ttatgtttcg cgatctccct
       61 gccaaggaat atctgctcgt cctttttccc aaaagtcagg ggtccgagct tctcgctcat
      121 tccccattcg gtaaccatcc tcctggcgag atctgttgca cgctccaggt cgtttcccgc
      181 gccagtggtc atgtggtgaa ggacaagctc ttccgccgct ctccctccca gaagcacatt
      241 gagcgtcttc aggagataat cctttgaata ggtgtacctg tcatccaggg gaagctgctg
      301 cgtaacgccc aatgcacgtc cccttggtat tatgctcacc ttatggattg ggtctgtccc
      361 gggggtcagt tttgccacaa gggcgtgtcc tgcctcatga tatgcggtgt tcttcttctc
      421 ctcttcactg atgatcatgc tcttccgctc cacgcccatg agcaccttat ccttggcagc
      481 ctcaaattcc ggcatgtcca ccttggactt tgatttcctt gccgcaaaga gggctgcctc
      541 gtttataagg ttcgcgagat ctgcacctga aaaccccggc gtccccctgg caatcaactc
      601 aaggttcaca tcgctgtcca gaggtatctt gcgcgtatgc accttcagta tctcaagtct
      661 tcccttcaca tcaggtatgg gcaccaccac ctgccggtcg aatcgtccgg gcctcaggag
      721 tgctgggtca aggacatcag gcctgtttgt cgcagcaagg aggatcaccc cttcagtgcc
      781 ttcgaacccg tccatctcga cgagaagctg gttaagggtc tgctcacgct catcatgtcc
      841 cccgccaagc ccggcgcccc tatgccgtcc cactgcatca atctcatcaa taaacacaat
      901 gcacggcgca cttttcttcg cctgctcaaa gagatccctt actctggatg cgccaacacc
      961 cacaaacatc tctacaaagt cagaaccgga tatgctgaag aagggcacgc ccgcttcgcc
     1021 ggcaatcgcc tttgcgagga gagtcttgcc ggaaccaggg gatccgacga gcaatacgcc
     1081 tttgggtatc tttcccccca gtttggaaaa cttctgcgga tctttgagaa aatcgataat
     1141 ctcctggact tccgcctttg cctcctcaat accggccaca tcggcaaagg tgatcttcac
     1201 cgacttatcc gatacgagcc tcgccctgct ctttccgaaa gagagtgcct tgtttccccc
     1261 tgtctgcatc tgccgcataa agaatatcca tacaagggcg agaaagatga tcggtcccca
     1321 ggagaaaaag aaattcacgt accagggatt ctgttcaggg ggctttgcgg caatcttcac
     1381 gccctttgtc ataagctcgt tcacaagctc aggatatccg gccgaatacg tcctgaattt
     1441 cttcccgtcc ttaagcctgc cggtaatgag gttttccttt atcaccacat cttccacctc
     1501 tccggccttt accttggtca taaagtcaga gaatatgacc tcatcctcca tttttttggg
     1561 tgtgctgacg agattaaaca ggagtatcat gatcaaccca atcatcagcc agataaacag
     1621 acttctgtac acgctattca atgcctttat cctccgcatt ctattgtaac atacaacctg
     1681 tcctttataa aagaaatacg gcgttacctt atctcattta tgaaaattaa gaaaagactc
     1741 gttcccaagg tgctccttct gaacctctat caacctctta attcctccat ctgcaagggc
     1801 aaggagcatg ccaagaccgt ccatagaaaa aggcgctttc tccgaagtgc cctgtatttc
     1861 cacgaacctt ccatccccgg tcatcaccac attcatatcc acatctgcag cggaatcctc
     1921 cgcatagcag aggtcaagcc ttggctcgcc gcccacaatc cccacgctca ccgcagcaag
     1981 gtggttcgtg agtggcattt tgtctattat cccatttttc atcgcagatc tcaaagcatc
     2041 tgcaacacag ataaaggccc cggtaatcga ggctgttctc gtaccgccat cagcctgtat
     2101 cacatcacag tcaacccata cggtacgctc tccgagggac tcgagatcga ttaccgccct
     2161 gagggtcctg ccgatcaatc tctggatctc atgggtcctg ccccctattt tgccggtgac
     2221 agattccctc gtcatcctcg tctgggttga ccggggaagc ataccgtatt cagcagtaac
     2281 ccatcccttc ttctgatccc tcaggaacaa ggggaccctc tcctccaccg atgctgagca
     2341 gatcaccttg gtatctccca tctcaaaaag cacagaccct tccgcagtct ttaagaaatt
     2401 gcgtgtgact ttcacttctc tcatctgacc gttctctctg ccgtcaggtc tcatcaatac
     2461 cctccttaca ggtcattgac caacagatat tttttcgata tgcgcgatct tttcacccaa
     2521 aaatctctca ccgaccttac gaaatctctc gggtgcatcg gtgacgtaga actctcgcaa
     2581 gggttcacgg gagggatttc cctctatgcc attcctcacg agcgtctctt ttatctcgct
     2641 cgccgtttcc gttgcagaat ctataagtga aatgcctcct cccataacct tgccgatcac
     2701 atccttgaga agcggatagt gcgtgcaccc caggaccagg gtatcgattc ccttttccct
     2761 caagttcttg aggtatcttt ccgcaaccat cgtggcgatc gcatcgtcag tgagcccctc
     2821 ctcagcaagg ggcacaaaca ggggacaagg aagactgaac acccgaatgc tcgcatccat
     2881 cgcccttatg atccgggcat aggcgccact gttcacggtc gcctcagtgc ctattacacc
     2941 gacattcttg ttcctcgtcg ccctcaccgc agcccttgac cccggctcaa taacgccgat
     3001 cacagggatg gagaggcttc cttttatctc ctcaagactc actgcagaga cagtattgca
     3061 ggcaaccaca agaactttta tgcccatgga cacaaggaat cctgtatttt cgaaggagta
     3121 gcgtgtcact gtctcggctg atctgatccc gtacggaacc cgtgccgtgt ctccgagata
     3181 caccgtgctc tcgcgcggga gcttcctgaa tacctctttc aggaccgtca gtcctccaat
     3241 ccctgaatca aaaaccccga tgggtctgtt attcataggc tacctgagtt ctgtaagggt
     3301 atttttcagt ggataaaggg catagagatg acccccgatg ctctccattt ccttctcttc
     3361 aatgagaatc ttcacatcgt ctacccccgt gacgttggag attatactct catagagccc
     3421 ctttaagagg agaaactctg acaatgcatc cccatgaaag ttccttctga actcgtccga
     3481 gagatccaca tagagtattc catcgttccc aaaatatatt cctaacattt tggtgcctgg
     3541 cggcacatcc gatttttccc tgtttgaggg acccttaagg aattcctcca caacctccgc
     3601 agcgcttacc gtaacagatc ccagtctctt cactcgcctt tcctctatga taagtcgccc
     3661 ttcagacggg aaatataccc ttatgaagga agaatcgtca gcctgtttct ggggctcgga
     3721 tggtgcttct cctttcggtt tcatcccaaa attccctgcg aaatagaaaa atcctcccgc
     3781 gatccctccc gaaaaaagaa ggatgaagac aaagacccac aagattcttc tcttattgat
     3841 tgacataaag tgcgataccc ctgaaaagcg cgtccaggac ccttgccttc ccagcctgat
     3901 cagaaatggt cgctgccggg atctcgatca ttaccgcagc cgcgcctact gaggaaagca
     3961 acggcagttc catctttctg aaaatgacat ctttcttgaa ctcgtccttg atcgccttac
     4021 cgatcccctc tgcaagggcc ttgcttttct cgatgaaccg cctctgccgc accatgatgc
     4081 tgtactcatt catagtgtca aaagctgccg actctgccaa agaagagtag accgcaaagc
     4141 tctgtgacgg cgtaaggtga atgctgataa atatgtccgc cgatttctgg tttgcgaaca
     4201 tcgccctctc ggtaagggac aaaaactggt ctgccttcct tgtgagaaat gcggtcctgt
     4261 tcctttttga cagcgaagat tccatagcgc gcgccaagga aagggtggcc tccttttccc
     4321 tgagctcgcc tgagaggatt cccagatcgt atcccccatg tccgggatca atgactatcc
     4381 ggatgttggg aatcaggggc agggatgaac cttcttttgc cccgggttga gagctttcct
     4441 ctttcgtaag ctggacaatg tctatggaaa gacggggggg agacgtgagc tggagcagtt
     4501 tcgtcttaaa gggataactc agatttatca cataagttct gcccttcagg gatgcgtcca
     4561 tgtccgggct tccctgaacc gtaagggcca gattggacgg gaactggacc tttatctggt
     4621 tctgtgttga cgtgaccact gtgttcctga caaaggattc atccggtgcc tcaaacacta
     4681 cacggcgaac cccttcatgg gtgctgaacc tcatggatac ctctgtcttt ccctctattg
     4741 cgtaaactga ttcgaacata ggggcatctg tcatgaaaag agaaatgccc agaacaataa
     4801 gaaagacttt agctgccatg ggatataatt tcccatatat gagataagtt ttgcaatatt
     4861 gcagttctct ctacccatca aatcaccgca aataataaag aaaaatgtgt ttcatattga
     4921 caaataataa agaacatggt attaatgtat gcagcaatta tataaccact ttatcggagg
     4981 gtaattatga atacgcacaa atcacggttt atgttcttta tctctgtcgc agcaggcgtg
     5041 cttctgttct tttcccagtc tgccttttct tcggagtcca caattaccat ccttggtgat
     5101 gtggtcggga atggtgatac ttatatgaag gccgcattta aacaagacat acctttgaga
     5161 ggcaagccct atccactgat tgacggggca catctccggg caggtgatgg catgatgtca
     5221 gtggtcttca gggatggggc caagctggag gtgggaaagt tctctgacct tgtcgtaaac
     5281 ggttcaaggg gaagttatat cgtcaacatg gggagcggaa aaatcggatt tgttatacct
     5341 caggggactt ctttttctgt aaagacaccc aactcaacaa tccagacaca atcggctgcc
     5401 ccagcgattc agaaggcgag cctctcctct cagagtacta cagtgaaggg cgtagtgagc
     5461 tacgatggca aaggaacaaa ggttatggca gtgagcggaa cactcacagt gagaagtggg
     5521 atcggcgttg tattgcagac agtgtcggaa ggcaatgcaa tttacatcga tggcaaggat
     5581 ggcgggagaa taaggacagc acagatggct acaacacctc cgccgtctcc gccgccgcct
     5641 cccccgccgc caactcctcc cagtccgcct ccgccgccgc ccagtggtac agcaggagaa
     5701 gatgcgatag ttgctggcag tgtggccggt gccggtaccg gggcttacat actcacccgg
     5761 caggaatata gtgtaggggt gactcccaaa gagtaagcgt tggattttga aaccatactt
     5821 ttttgcagat aagcgcctct ttatgataaa gaggcgctta ttcattaatt aatatgtaat
     5881 gtttccgtag ttaatttttg attgattttg cctgaacagt tgaagtaaca ttaagtatga
     5941 agaaattggt agtagttgcc gcagtactag cccttttctc ttttgttctc ataggatgtg
     6001 gcggagggag ccaaccggtt caagttcaaa gtttggattc cgagatcaag caggaaggga
     6061 attcaacaag aatgaatgac ctgctcctta aagcagcaat ggcgagaccc tcgaacagag
     6121 atgctgatta catcataggc cctgatgacg tcctcgatat agacgttttt caagccgaag
     6181 agctccacag aaccgtcagg gtaagttacc agggatacat tggcatgcca ctgatagggc
     6241 agatcaaggc gagcgggtta actcctctgc aactcgagca gcttatagcc caaaaactcg
     6301 aaaagtatat gcaggagccc cgggcgagtg tagcgataaa agaatacaag tcacaaaaaa
     6361 tcagtgtaat aggggcagtg gggaagtccg acgtatactc ggtgacaggt cagaaatatc
     6421 tcgttgatat gctgatcatg gcagggggta ttagcggagg cgcctcggaa atctgcttaa
     6481 ttctgcgtcc ttcagattcc gagatggcgg gagaaaaaaa gacagagact ttagcgatta
     6541 atctcataga actgcttgag aagggagatt tttctttgaa tatcccggtc ttcagcggag
     6601 atatcataaa tgttttgaaa aatggtatcg tgtatgtcga tggtccagta aacaggccag
     6661 gaccatacgg cttccagatg aagatgactt tgagggaagc gatcatcatt gccgggggtc
     6721 tgagattcga agctgactct tcggatgtga agatattaag agataaaggg gatgggacac
     6781 gagaggtcat tgcggtcaat tacgacgata tcacccaagg caagaccaat gatttcccca
     6841 taaaggaaaa cgatatcatc atcatgggca agaatggctt taagaacttt ctcgttactg
     6901 tttctggaat gctcagcggc ggcttgaaca caaccggtgg ttttgtagtt aacccggcaa
     6961 caccaattaa ataaacttca ccttttgcat caggcccggg attgataccg agaaaggccc
     7021 ctgacatgag tatggatatt ttcttttttt ttgatgagga gacaagacta ttatgaacaa
     7081 ttttgataat gcgccacagg taaacacaag cctgcaaaag gcagatgagt accctgttgc
     7141 atttccggca aattactcct tcgaacagga aagagagatt catctcaggg actatctgag
     7201 ggtcattctc agaaggaaat ggattgtaat cacatttttc ctcatttcag tcaccacggt
     7261 tattctcgga acatttatga caaagccaat gtacagatcc tccgttaccc tgaagatcga
     7321 aaaagactct ccccaggtgc tcaacatcaa ggaagcatat gaagttgata aatcatcaga
     7381 tgcgtattac cagacccagt acaagattct caggagcagg aatcttgcga agagggtcat
     7441 tcgggccatg aagctggacc agaaccccgt atttattggc tatgttccaa ccccaaaggc
     7501 agtaaccaat aaatcaattg atactcgctc caatacggat atggatgagg atttcagtcc
     7561 tgcggttgtt gacaggctta tcgcaggcct cggtatagaa ccccaagaca agtcaaggat
     7621 cgtcaagatc agctttgaca gccaaagccc tgaattagct gcaagtgtcc ccaatgcggt
     7681 ggcgaagtcg tacattgatt acaatataga gagtaaattt gatgccacgc tccaaacaag
     7741 agactggctt gaaaaacagc tcgaggatat gaaggcaaag gtggagagag ctgaagagac
     7801 attaaacaga tatgttgccc agaacggaat cattttcctc gctgacagtg ggtcgggtga
     7861 cacgaaggaa tcaaaaggtc agagcatggt aaacaaaaag ctctcggatt tatccaacca
     7921 gctcgttcag tcaacttcgg acagagttac aaaggaagta atgttcaggg aggcccagag
     7981 ggagg
//
LOCUS       DATKOU010000115         4890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3614732, whole genome shotgun sequence.
ACCESSION   DATKOU010000115 DATKOU010000000
VERSION     DATKOU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614732"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="aroB"
                     /locus_tag="VMU21_05275"
     CDS             complement(<1..851)
                     /gene="aroB"
                     /locus_tag="VMU21_05275"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HUO76972.1"
                     /translation="MEAIRVELGERSYEICIGPGILPQLGERLREFHFSRVALVSNPT
                     VYALYGNTLLDSLRKAGFDAAVVTIPDGEEYKELLWAYHIYGELLKHRLDRSSALIAL
                     GGGVIGDITGFAASTYMRGISLVQVPTTLLAQVDSSVGGKTGVNHPEGKNMIGTFHQP
                     RLVWADVDTLRTLPRREFLAGMAEVIKYGVIWDETFFNFLEKNREKILSLDSGTMQFI
                     IRRSCEIKAEVVSRDEREGGLRAILNYGHTIGHALETATGYTAHLHGEAVAMGMYCEA
                     LLSCLLG"
     gene            complement(839..1348)
                     /locus_tag="VMU21_05280"
     CDS             complement(839..1348)
                     /locus_tag="VMU21_05280"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HUO76973.1"
                     /translation="MKNIVLTGFMGTGKTEVGRELSTILGWKLIDVDDEIVKSKKMSI
                     NEIFSRLGEPAFRDMETEMIRKVSQKRNVIISTGGGAVLRQENRELLKTRGVVVCLTA
                     LPETILKRTSGNRERPLLQVEDPVQRIHDLLEARRPFYEKADITIDTEGKSPFHIAGE
                     ILERMGWKR"
     gene            complement(1407..2591)
                     /gene="aroC"
                     /locus_tag="VMU21_05285"
     CDS             complement(1407..2591)
                     /gene="aroC"
                     /locus_tag="VMU21_05285"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HUO76974.1"
                     /translation="MSRLRFLTAGESHGKALVGIMEGMPANLSLSPSDMDAELKRRQS
                     GFGRGGRMKIEADHAEIVSGVRWGKTIGSPIALLIENRDHKNWLEGMSPDGRHQGSIP
                     AVTRPRPGHADLAGVIKYHQQDVRNILERSSARETAMRVALGAVAKKFLSEFGIRVGS
                     YVTQIGDQRSAVSSQQLGEEEFFELFGRAEASSVRCPDEDATREMVQLIEDAMRKGSS
                     LGGVFEVFATGLPVGLGSHIQWDKRLDGKIGGALMSIQAIKGVEIGLGFEMAGGSGSG
                     VMDEIFYHGQKTGFYRKTNNAGGIEGGMTNGMPVLVRAGMKPIPTLRKPLSSVDIISK
                     EPFEAAYERSDVCAVPAASVIGEAMTALVIADAFLEKFGGDSMEEITANYKAYLEYVK
                     NL"
     gene            complement(2616..4451)
                     /locus_tag="VMU21_05290"
     CDS             complement(2616..4451)
                     /locus_tag="VMU21_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUO76975.1"
                     /translation="MKRVAYLFLVLFFVSCGSDNTTTISSDSSKSGTSQQISSPRVSV
                     QSVLSGMEKGKYREGELLVKFRSGVVAASSSKVHHVLGAVSLKRFTLVPNLDHVRLPE
                     GLSVKDAIIKYMEDPSVEYAEPNYIKRISKVPNDPLFAQQWGPTKIDAPGAWDTNTGN
                     NNVIVAVIDSGIDLKHPDLIANIGPGSNFISNTSVPQDDNGHGTHVSGIIGAVGNNGI
                     GVTGVMWNVKLMPLKFLDAEGNGFTSDEIDAIGFAVAHGAKVMNTSFAGSEFSCSEYA
                     AVSSANVLLIAAAGNETANNDTVPSYPASFSNPKDPNLIDPAVISKCGVSALPALPNV
                     ISVAATDQNDNLASFSNFGLNAVQVAAPGVNILSTIPLDLPPCTTNPFEPNYDYCSGT
                     SQATPFVSGLAGLIYSQNPNLSYLQVRSIIFSTVDILPSLNTKILTSGRINAFRALTA
                     PIVPRISISPLSNNFGTANVGSTSSPQTFTVSNPGTADLIIGQVTLTGVNAAEFSVGG
                     DGCSGHTIAPLFNCTIQATFSPASAGAKNASMSIPSNDVASPTTAVSLVGIGAAQSSS
                     SGGGGGGGCSIGGRQNAPSAVADSLVILTPLLLVTILRRRKKTKR"
     gene            complement(4483..>4890)
                     /locus_tag="VMU21_05295"
     CDS             complement(4483..>4890)
                     /locus_tag="VMU21_05295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUO76976.1"
                     /translation="LRFSVKDAATGIVVSAVVLLPFWYLSSHLGRPFASLPVNAVIFQ
                     LLGVAVPEEVYFRGFLQQRLGNTLKAVLAVSLLFSFMHLPQFIFYGDTYSLLTFFPSL
                     VMGLLYWRTSNVLPSAIFHFCSNIVFLGVYDIL"
BASE COUNT         1191 a         1370 c         1148 g         1181 t
ORIGIN      
        1 aagccaagca gacaagagag cagcgcctca caatacatcc ccatggcaac tgcttcgcca
       61 tgaagatgcg cggtgtaccc agttgcggtc tcgagggcat ggccgatggt atggccgtag
      121 ttcaggatcg ccctcagccc cccttccctt tcgtccctgg agaccacctc ggccttgatt
      181 tcgcaggaac gcctgatgat gaattgcatc gtaccgctgt ccaggctcag gatcttttcc
      241 ctgttctttt caaggaaatt aaaaaatgtt tcatcccata tcactccata cttgattacc
      301 tcggccatcc ccgcaagaaa ttcacgcctt ggcaatgtcc tgagtgtgtc cacatcagcc
      361 catacaagcc tcggctgatg gaacgtgcct atcatattct tcccctcagg atgattcacc
      421 cctgtcttgc cacccacaga gctgtccacc tgggcaagga gggttgtggg cacctgaaca
      481 agcgaaatgc ccctcatgta ggtggaagcg gcaaagccgg tgatatcgcc gatcacccct
      541 ccccccaggg caatgagtgc agacgacctg tccagtctgt gcttcaggag ttccccgtag
      601 atatggtatg cccagaggag ttccttgtat tcctcaccat cagggatggt caccactgct
      661 gcatcaaacc cagcctttct cagggaatcc agaagcgtgt tcccgtagag cgcgtatact
      721 gtgggattgc tcaccagtgc aacacggctg aaatgaaact cccgcagcct ctcgcctaac
      781 tgagggagaa tgccgggacc tatgcatatt tcgtagctcc tctctcccag ctctaccctt
      841 atcgcttcca tcccattctc tccaatatct cgcctgcgat atgaaagggg ctcttcccct
      901 ctgtgtcaat cgtgatgtca gccttctcat aaaacggccg cctagcttcc agcaaatcgt
      961 gaatcctctg gacgggatct tccacctgga gcagcggcct ttccctgttc ccgctcgttc
     1021 tcttaaggat tgtttcaggc agtgcggtaa ggcacaccac cactccacgt gttttcagga
     1081 gttccctatt ctcctggcgg agcactgcgc cgccaccagt ggagatgatc acattccttt
     1141 tttgtgagac ctttcttatc atttctgttt ccatatcccg gaatgcgggt tcgcccaacc
     1201 tgctgaatat ctcattgatg ctcattttct ttgacttcac aatctcgtca tccacgtcaa
     1261 taagcttcca gcccaaaatg gtggagagct ccctcccaac ctcggtcttc cccgtcccca
     1321 taaatcctgt gagcacaatg tttttcatat gagaataccc ttaattccct caccggatgg
     1381 acgttcacaa aaaggatggc aacctgtcaa aggttcttca catactccag atatgccttg
     1441 tagttcgctg tgatctcttc catgctgtcg ccgccgaatt tttcgagaaa tgcatctgca
     1501 atcacaaggg cagtcattgc ctcccctatt accgatgccg cgggcacggc gcacacatcc
     1561 gaacgctcgt aggcagcctc aaagggttcc ttggaaatga tatcaacaga actgagcggc
     1621 ttgcgtaatg tgggtatggg cttcatccct gcccttacaa gaacaggcat cccgttggtc
     1681 atgccgccct ctatgccccc ggcattgttc gtcttcctgt aaaaaccggt cttctgaccg
     1741 tgataaaaaa tctcgtccat gaccccggag ccggagcctc ccgccatttc aaagcccagg
     1801 cctatctcca cacccttgat tgcctgaatg gacatgagcg caccgccgat ctttccgtca
     1861 agccttttgt cccactggat atgacttccg agacccaccg gaaggcctgt ggcaaatacc
     1921 tcgaacacac cgccaaggga actccccttc ctcatggcgt cttctatgag ctgaaccatc
     1981 tctctggtgg catcttcatc aggacacctc acagaagagg cctctgccct gccaaataat
     2041 tcaaaaaact cctcttcccc aagctgctga ctgctgaccg ctgaccgctg atctcctatt
     2101 tgcgtcacat agcttcccac cctgatgccg aattccgaga ggaatttttt tgccactgcg
     2161 cccaacgcaa ccctcatggc agtttccctg gcgctggaac gctccagtat gttcctcaca
     2221 tcctgctggt gatattttat gaccccggcc aggtccgcat gcccgggcct cggcctcgtc
     2281 actgccggga tcgaaccctg gtgccggcca tcaggagaca taccctcaag ccaattcttg
     2341 tggtccctgt tttctatgag gagggcgata ggtgaaccaa tggtctttcc ccagcggacg
     2401 cccgagacta tttcagcatg gtctgcctca atcttcatcc ttcctcccct tccaaagcct
     2461 gactgccgtc tcttcagttc agcatccata tcggacgggg aaagggaaag gtttgcaggc
     2521 attccctcca taatccctac aagggccttg ccgtgggact cgcctgcagt aagaaatcta
     2581 aggcgtgaca taatactttt tgtccaactc ctctgctatc tcttagtctt ttttctgcgt
     2641 ctcaaaatcg tcacgagcag gagcggcgtt agtattacca aagaatcagc cactgcgctt
     2701 ggcgcgttct gccggcctcc tattgaacat ccgccgcccc ctccgcctcc ggaggaagat
     2761 gactgagcag ctccaatgcc gacaagggag acggctgttg tgggagaagc aacgtcgtta
     2821 gacggaatcg acatactggc attttttgcg cctgctgatg ccggagaaaa cgttgcctga
     2881 attgtacagt tgaacagcgg cgcaatggta tgccctgaac acccgtcgcc gcccacgcta
     2941 aactcggcgg cattcacacc agtaagagtt acctgaccaa taatgaggtc agcagtcccc
     3001 gggtttgata cagtaaaggt ctgcggagag gaagtgcttc caacattcgc agttccaaaa
     3061 ttattgctta agggagaaat agagatacgg ggaacaattg gcgctgtcaa tgcacgaaag
     3121 gcattgatcc ttccggatgt tagtattttt gtattgaggg atggcaatat atcgacggta
     3181 ctgaatatta tgctgcgaac ctgcaagtac gaaagattcg gattttgact gtagatcagt
     3241 cctgcaaggc ccgacacgaa tggtgttgcc tgggaagtgc ctgagcaata atcataattc
     3301 ggctcaaagg ggtttgttgt acagggaggg aggtcaagag ggatggtgct caggatattc
     3361 acccccggtg cagcgacctg aacggcatta aggccgaagt tgctgaatga tgccaaattg
     3421 tcattctgat cagtcgcagc aacggaaatg acattgggaa gggccgggag agcagaaacc
     3481 ccacatttac taataacagc gggatcgata agatttgggt ctttcggatt actgaagctt
     3541 gcaggatatg acggcacagt atcattattc gcagtctcat ttccggcagc agcaataagg
     3601 agaacatttg ctgagctgac tgcagcatat tcggaacaag agaattcaga acctgcaaag
     3661 ctcgtattca ttaccttagc gccgtgagca accgcaaacc caatggcatc aatctcatcg
     3721 gacgtaaatc cattgccctc tgcatcaagg aactttagtg gcatgagttt cacattccac
     3781 attacgcctg tgactcctat cccattgttt cccaccgcgc ctataatccc ggacacatgc
     3841 gtcccatgcc cgttgtcgtc ctgagggaca ctggtatttg agataaaatt agatcccgga
     3901 ccaatattgg caatcaggtc aggatgctta agatcaattc ccgaatctat gaccgcgaca
     3961 atcacattat tgttccctgt gtttgtgtcc catgcgcccg gtgcgtcaat ctttgtgggg
     4021 ccccactgct gggcgaataa tgggtcattg ggaaccttcg aaatacgctt gatatagttc
     4081 ggctcagcat attcgactga agggtcttcc atatatttta tgatcgcatc cttcacagag
     4141 aggccttccg gaagtctgac atgatcgagg ttggggacaa gggtaaacct cttcagcgac
     4201 accgcaccca aaacatggtg tacctttgat gaggaagcgg ctacgactcc tgacctgaat
     4261 ttcacaagca gttctccttc cctgtatttg cccttttcca taccagaaag gacagactgc
     4321 acagagaccc ttggagagct gatctgctga gaggtacctg actttgagga atcagaagaa
     4381 atggttgtgg tgttatcgct tccgcaggac acaaagaaca gaaccagaaa aaggtacgca
     4441 actctcttca ttttttccct cacacgagtt tatttggcga ttttagagta tatcataaac
     4501 acccaaaaac acaatattcg agcagaaatg aaagatggcg gatggcagga cattggatgt
     4561 ccgccaatag agcaggccca tcaccagaga agggaagaaa gtgaggagtg aatacgtgtc
     4621 cccataaaag ataaattgcg gcaggtgcat gaatgaaaag aggaggctca ccgccaggac
     4681 agccttgagg gtattgccga gcctctgctg gaggaatccc ctgaaataca cctcttcagg
     4741 tactgcgaca ccgagaagtt gaaagatcac ggcattgaca ggtaacgacg caaacggcct
     4801 tcctaaatgg gaggagagat accagaaagg caacaacact acggcagaaa cgacaattcc
     4861 cgttgccgcg tccttcactg agaacctgag
//
LOCUS       DATKOU010000116        17771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4386587, whole genome shotgun sequence.
ACCESSION   DATKOU010000116 DATKOU010000000
VERSION     DATKOU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 17771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17771
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4386587"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VMU21_05300"
     CDS             complement(<1..677)
                     /locus_tag="VMU21_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76977.1"
                     /translation="MKRIRVLATIIVLSCSPLFLNACASSDKKLVKSIPSAEPIKVCR
                     YETPGIMKSTGTETFLMAAIEMGAPGGSALLVLGDEYNRARGSGTQSKIPDFGSLVMD
                     KFVEQVKVAKPDWPQLSVILTPLQEDFSEKCMVIEFKVSRVAYGSLDLTKGGIVFERG
                     LDKGLVTNGFLSKTMVTMKDSEGEVIWEKSYIYLSDNFGREKSLDELEADDYKLLKEE
                     MAFAAEKT"
     gene            complement(885..1511)
                     /locus_tag="VMU21_05305"
     CDS             complement(885..1511)
                     /locus_tag="VMU21_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HUO76978.1"
                     /translation="MKNIYRVQCIFMFSILALPILSGCASTESSRFYTLNTIKNPAGV
                     QRVAASEQRITVGLGPIEIPDYLDRPQIVTRNSDNEMKIDEYGRWAGSLKEDINRVLA
                     ENLSILLSQNRVSVVPSLSGVSFDYRVIIDISRFDIMPDGNVALRAQWSIIKREGRTV
                     IVTRESGYTVEAQDKGYGGKVSAMSRALEMLSKDVADSIKPVARRIKK"
     gene            complement(1513..2307)
                     /locus_tag="VMU21_05310"
     CDS             complement(1513..2307)
                     /locus_tag="VMU21_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUO76979.1"
                     /translation="MSKKANKTLIGAFVIGAIALIVAGVVIFGSGKLFTSLSKFVMVF
                     DGSVKGLNVGSPVIFRGVKIGEVTGLQLRLNTQDLTAVIPVYVEIDTRTLHVSEEGQA
                     IMRKRALLKPLIAKGLKGQLQMQSFVTGQLMINLDFYPDKPIKLTGIEKKYLEIPTVE
                     APMDELAKTIENLPLKEIFAKVDHAMDGINKMVNAPGAHNALGSFNDTMLQTKESLND
                     YSDLRFELSSMLRELSAATRSIHYLSDYLERHPDALLRGKASPKGE"
     gene            complement(2356..3147)
                     /locus_tag="VMU21_05315"
     CDS             complement(2356..3147)
                     /locus_tag="VMU21_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO76980.1"
                     /translation="MDELVKKTVEKKEPHIIVEGLTMAYGDFLIQRDLSFTINKGDIF
                     IVMGGSGCGKSTLLRHLVGLQTPAKGRVLYGDVSFWDAEPKEQERIMRSFGILYQSGA
                     LWSSLTLGENIALPLQQYTDLKPGQIKEIISLKLALVGLSGFDDYYPSEISGGMKKRA
                     GLARAMALDPDILFFDEPSAGLDPISAHLLDELILELRESLGATVVIVTHELASIFAV
                     GNNSVFLDPDTKTMIAQGDPKKLLAESKDPKVISFLTRGEGMKQP"
     gene            complement(3154..4305)
                     /locus_tag="VMU21_05320"
     CDS             complement(3154..4305)
                     /locus_tag="VMU21_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO76981.1"
                     /translation="MSTDKSVSTVRSLTFERPSENRVLVRITGEWKLKNSLPTADEVM
                     KEMRAGQKVRQIAFDAGDLKGWDSGLLTFLIRVMDLCSAADIAVEKDGLPKGVQRLLS
                     LATAVPERKGARREAERISFVEKVGDSAIAFWRSILEMLEFIGEASVAFVRVFTGKAK
                     FRPSDLFLTIQECGAQALPIVSLISLLVGLILAFIGAIQLKIFGAQIYVASLVGIGMV
                     RALAAVMTGIIMAGRTGAAFAAQLGTMQVNEEIDALKTLGVSPMEFLVLPRMLALFFM
                     MPLLSLYADLMGVLGGLIIGVSMLDLNFMEYFIKTRESVSMNDLWVGVFSAAVFGVLV
                     ALAGCMRGMQCGRSASAVGEATTSAVVTSIVSIVVAMALITVVCNVLHI"
     gene            complement(4284..4475)
                     /locus_tag="VMU21_05325"
     CDS             complement(4284..4475)
                     /locus_tag="VMU21_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO76982.1"
                     /translation="MAMVNSSIAYALGENEKAEKSLQAALKKGVGEISPPGRWLRKIS
                     GKAMVNKLFERRACPQINP"
     gene            complement(4945..6111)
                     /locus_tag="VMU21_05330"
     CDS             complement(4945..6111)
                     /locus_tag="VMU21_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO76983.1"
                     /translation="MGIPYSYSIRNLLTRKLTTVLTALGMALVVFVFAATLMLSEGLR
                     KTLVDTGSYDNVVVIRKAANSEVQSGVERLKASIIETQPEVAIGENGEPLLAKELVVL
                     INLPKRGSSKPANVVIRGIGAGSLLLRPQVRLVEGRMPKPGSAEVIAGGSIAKRFQGG
                     GIGETVRFGMRDWTVVGIFDAGNTGFSSEIWGDVDQLMQAFRRPVYSSVIFRLRDSTE
                     FEGFKTRIEGDPRLSLEAKREKIFYAEQSEGMAKFLNILGMTLTVIFSLGAVIGAMIT
                     MYAAVANRVTEIGTLRALGFQKGSILATFVLESLFLGFIGGCIGIFFASFMQLITIST
                     MNWQTFAELAFSFTLTPDITGKSLAFSLIMGFAGGLLPAVRASRMSIVEALRAT"
     gene            complement(6112..7269)
                     /locus_tag="VMU21_05335"
     CDS             complement(6112..7269)
                     /locus_tag="VMU21_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUO76984.1"
                     /translation="MFFLKLLFRNAFRHKLRTTLTVVGVAIAILAFGLLRTLVGLWYL
                     GVERSSATRLVTRNAISLVFTLPISYKDRILQIRGVKSISYGNWFGGIYADEKHFFAN
                     YAVEPRSYLELYPEFVLPPDQKAAFLRDRKGCIVGAKLAELHKWKLGDIITLKGTIFP
                     GQWEFVLRAIYKGADKSTEERALMFHWDYLNETMKKTVPRRADQVGFYMIGVTNKDLA
                     PEVAVEIDRTFKNSLAETMTETERAFQLGFISMTEAILIAIQIVSYVVIVIIMVVAAN
                     TMAMTARERISEYATFKTIGFGKWHIGGIVFGESLVIAMTGGIIGALLTFPAARWFEV
                     ELSQYFPVFSVSPFTIYLDLLAAMGIGVVAAVFPTWRSATIRIVEGLRRIG"
     gene            complement(7256..7951)
                     /locus_tag="VMU21_05340"
     CDS             complement(7256..7951)
                     /locus_tag="VMU21_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO76985.1"
                     /translation="MEVPRQPIVEIKNLNKTYRRGSQVISVLNDITFDIEEGEFLALM
                     GPSGSGKTTLLNLIAGIDKPDSGSVRVGEVEITSLSETELARWRASHVGFIFQFYNLI
                     PVLTAFENVELPLLLTGLSRRERREHVETALYAVNLSDRMDHYPSQLSGGQQQRVAIA
                     RAVVADPTILVADEPTGDLDRVSAREILDLMERLVHELGKTIIMVTHDPRAAEKAHMV
                     RNLDKGVLNNVLS"
     gene            complement(7952..9166)
                     /locus_tag="VMU21_05345"
     CDS             complement(7952..9166)
                     /locus_tag="VMU21_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUO76986.1"
                     /translation="MAGEDLSKLKIDKSPAARHLKRGKPFHKIGAMLLVALIALLFVK
                     GAFSPAVQVEVATVTQVYPSRTFTLLNASGYVVAQRKASVASKATGNLEALYVEEGSR
                     VEKNAIIARLENRDVVASRDQAVANLNLARSSLEQARAELHDATLAYDRARDLVAKGF
                     IAKADYDTAEARYQKAVAGVAASEAGVKANVAALRAAEVAIEYTLIRAPFDAVVLTKN
                     ADVGDVITPFGAAANAKAAVVTIADMNSLQVEADVSESDLEQVKPGQPCEIQLDALPE
                     SRFRGEVHMIVPTADRSKATVMVKVRFLEKDPRILPEMSAKVAFLQRPVTAEEQKPRT
                     AINPAAVLNRNGKKAVFLIKDGRAVETPVTLGSQIGDMVEVLGGVKAGEKVVLKPLNK
                     VKGGDKVKVAEK"
     gene            complement(9339..10685)
                     /locus_tag="VMU21_05350"
     CDS             complement(9339..10685)
                     /locus_tag="VMU21_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO76987.1"
                     /translation="MIRVLAVDDEEPFRRLLKKELARKGFFAEVAPDAETALRLLKES
                     FFDVMLLDILMPGLNGISLMKKLKADPAPPVIIVLTGKATVETAVEAMKHGAYDYLTK
                     PYKLDELVILINRAYEYGKLKIKNEQFQQELLRKETAVDFVGGSSQVTHILALIRKIA
                     PTDSTVFIQGESGTGKELVANTIWQHSKRSMFPLIALNCATLSENFLESELFGYEKGS
                     HSTAYQAKYGLFEVADKGTLFLDEIGEIPRGLQAKLLRFLDSGEFRRLGGNKTLKVDV
                     RVITATNKNLAESVAAGGFRQDLYYRLNVINITIPPLRERREDIAELSRHFLKRYSRK
                     LSKTISGFSPEALEGLHSYHWPGNVRELENVVERAVTLCDTDTIHSHDLSIPLLSSVK
                     ETGMHASLEHMEKEHILKVLRESRGNQSKASQLLGIDRKTLYLKLKKYGVAEFPEK"
     gene            complement(10682..13144)
                     /locus_tag="VMU21_05355"
     CDS             complement(10682..13144)
                     /locus_tag="VMU21_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUO76988.1"
                     /translation="MRAELTEKPGFILEVFDFLNIPAIMIDTQRRVVLANTAANTIFE
                     VMPDSMSGREVSEFVFPQSFPPSWSGKEEGGSQFESLCKKKNGKSFPARISFVPYPHD
                     DLQIVTVQDMSVQKKLQQRASQRAKELAVFNNFAKTLARYTGAGKIIQETVYMLRYLM
                     EADNGWVYLMDDASGELRLVAHQGLPKTLLNEISCRKAGEGFIGKVFSSGRPLIVESA
                     SEDPRVLHKGAGMQSMAGVPITSKGTPLGVLGLASRKESYFTSLDTQLLTTIASELGV
                     AIENTKLIGQLKDRMKEIKLINELSSMINSSLSMRTVFRIMMSEIKKLIDYDRGSILL
                     YNEKENNLLIFALDTNMRTILRRGVRAPLDGISAGWVIKNNRPWINDDLEKGIAFTLD
                     KKLLDEGIRSTISIPLVQDKILGVFNLDSTRPSQYSERDLHLLLSVSKHISIALENSL
                     LFEEISREKKEWEQTFDAITDMVWIEDGRQRVIRANNALLARSGVSLVEVVGKHCGEV
                     LERIGINPVESCTRDALSMERPSFAELKGSGGTLFHFWAYPLTDDEGQLYAIVRYLTD
                     VTAQKRLEQQLIRADKLASLGTLVAGIAHEINNPLGIIAGYSEALLERAKDISLLRMK
                     EFEDFPEYLETIHNEIFRCKRILNTLLEFARPHGGTFRELDINELIKEVILLVNHKAT
                     KFNHDIEFRLSRDLPKISADPGSLRQLFMNIIINSLYFTPEGGSIIITTQPGISASDH
                     RGETAEMIEVSISDTGPGILHDIIDKIFDPFFTTKPVGEGTGLGLAICHKIVEEHSGN
                     IYAESEAGRGTTFTIHLPAKTA"
     gene            13233..14039
                     /locus_tag="VMU21_05360"
     CDS             13233..14039
                     /locus_tag="VMU21_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUO76989.1"
                     /translation="MTEKDLSFFQSWFSRYCRSFYTGNEEDRRNLLLKKDHTRHVCEN
                     MRRIAKDLSLESAEEMLAETIALFHDVGRFPQYAEYKTFRDSISVNHATLGTEVLSRE
                     GVLETLPKNEQRIILQAIKFHNAYAVPDIRDNDMLLFLKLIRDADKLDIWRVFIEYLA
                     LPEKERASAVGLGLPDVPEYSENVLSCVLSKEVASLSNLKTLNDFILMQLSWVYDLNF
                     RTSFILLEERNYINKLISTLPSTEAIGKLSPFLGEYVREHIQAAPSHHDL"
     gene            14036..14827
                     /gene="rsmA"
                     /locus_tag="VMU21_05365"
     CDS             14036..14827
                     /gene="rsmA"
                     /locus_tag="VMU21_05365"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HUO76990.1"
                     /translation="MSRRRLGQNFLFDPSILNRMIEIARLSPEDTVVEIGPGHGRLTA
                     MLAKKVKRVIAIELDATLYERLRETLLAYDNIEVVHADALHYPYDTLDTFKVVANIPY
                     YITTPIIFRLFEHRNRLSSITVTVQREVARRIVAGPGGKDYGVLSLMVQYYGQPKLEF
                     VVPKGAFRPVPKVDSAVVHIELHEKPPVAVRDERFFFRAIKTAFSQRRKMLLNALKPI
                     SGDIKERLVFAGIDPTRRPETLSMEEFARLSDELLSKHRDSSESG"
     gene            complement(14840..15340)
                     /locus_tag="VMU21_05370"
     CDS             complement(14840..15340)
                     /locus_tag="VMU21_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HUO76991.1"
                     /translation="MSRQQKSKMPKFVVHEHHASHLHFDFRIEMEGVLKSWAVPKGPS
                     MNPKDKRLAIMVDDHTLEYGSYEGIIPEGNYGAGAVLIWDSGEYGYLGGNIRMGRLEF
                     SLKGKRLKGIFILARMSGKEKEWLLMKKRDEFAEDNFQMKTMLTPSLLKKLKTQTPPC
                     DASWDS"
     gene            complement(15592..16827)
                     /locus_tag="VMU21_05375"
     CDS             complement(15592..16827)
                     /locus_tag="VMU21_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HUO76992.1"
                     /translation="MNTALYLIDVTNRDGVQTSRILLPKLSKTMLNLYLDEMGVYQSE
                     IGFPTLKHEINYINANLDLVKAGGIKRLHLEGWCRALPEDVELSFKNCPGLRHLNISV
                     STSPIMIQGKFQGRKTWKDVVESMYKSAKLAKQLGVVTLGVNAEDASRTDLDKLIEFA
                     LAAKEAGADRMRYCDTLGVDDPITIYERIKALALATKVPIEMHCHNDLGMAGAVSVAG
                     AQGAFESNVDSYINTTINGYGERAGNCDLVSTILALKFSHALKAKIALDEHINLKTAW
                     KIAKYASYAFNIPIPIKQPGVGANAFAHESGIHADGALKDRRSYELYDPEDVGRGEPE
                     LLETGRIITTGEYGGLKGFRHAYDKLGISFHNDSEARRILELVQYANLHTQKPLTDDE
                     LRFIANYPEIVQKILVVQP"
     gene            complement(16824..17603)
                     /locus_tag="VMU21_05380"
     CDS             complement(16824..17603)
                     /locus_tag="VMU21_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO76993.1"
                     /translation="MSLSVKIRKKMDSFVLDVSWQMNNELAVLFGHSGAGKSLTLQMI
                     AGLVEPDEGKIRLGDVVFFESKAGTRLKPQDRHLGYVFQDLALFPHMTVYQNILYGGY
                     GISKTEKESMAVEMMRRFRIAELRDRLPGRISGGQKQRVALARALMRKPRVLLLDEPF
                     SALDASIRLGMGRLLKEVQHEFNIPVVLVTHDLHEARALADRMFVYSAGKIIQSGRPD
                     DIIHLPESQELMDLVESSAYRTQEPAAIWADPLRWELNRTP"
BASE COUNT         4160 a         4851 c         4297 g         4463 t
ORIGIN      
        1 gtcttttcgg cagcaaatgc catttcttct ttaaggagct tgtagtcatc agcttccagt
       61 tcgtcgaggc tcttttccct gccgaaatta tcagaaagat agatatagct cttctcccag
      121 atgacctctc cttcggagtc cttcatggtc accatagtct tgctgagaaa gccattggtc
      181 acaagccctt tatccaggcc cctctcgaag acaatgcccc ccttggtgag atccagggag
      241 ccataggcca ctctgctgac tttgaattct atcaccatgc atttctccga aaaatcctcc
      301 tgcaggggcg tgagaattac ggacaactgg ggccagtcgg gctttgcgac cttcacttgt
      361 tccacgaatt tgtccatgac gagggagccg aagtcaggga tcttgctttg tgtgccggaa
      421 cctcgcgccc tgttatactc gtcacccaga acaagtaagg cacttccccc aggggcaccc
      481 atttcgattg ccgccataag gaaggtctct gtccccgtgc ttttcatgat ccctggagtc
      541 tcataccggc agacctttat gggttcggct gatggtatgc tttttacaag tttcttatca
      601 ctggacgcgc atgcgttaag aaagagcggg gaacaagaca agacaatgat cgtagccaat
      661 acacgtattc ttttcatcta aggcctcctt ctggatctcc ccatcagtgt gatgcggaga
      721 aaagggataa actcggttaa tcctccccgt acccatacca ttaacttccc cgcgggatta
      781 cgggagccat ttatcgctct cttttctgat cggaaattaa accaaagcca acaaatgtcg
      841 cctgtcccaa tatcctccgg atggtgtaaa caccaaggat acctttattt ttttatccgt
      901 ctcgccacag gctttatcga atcagcgaca tccttgctga gcatctcaag ggcacggctc
      961 atggcagaga ctttcccgcc gtagcctttg tcctgtgctt ctacagtata tcccgattca
     1021 cgcgtgacaa tcacggtcct gccctccctc ttaatgatac tccactgagc tctcaatgcg
     1081 acatttccat caggcatgat atcaaaacgg ctgatatcta ttatcaccct gtaatcaaat
     1141 gacacgcccg agagagacgg cacgacagag acgcggttct gagagaggag aattgagagg
     1201 ttttcggcga gaacccgatt gatatcttcc ttcaaagaac cggcccacct gccatattcg
     1261 tcaatcttca tctcattatc gctgttccgc gtcacaatct gaggtcggtc cagataatcg
     1321 gggatttcaa tcggtcctag accgacagtg atgcgctgtt ccgaggcagc cacccgctga
     1381 acgccggcag ggttcttgat ggtgttcagt gtataaaaac gagatgactc agtactcgca
     1441 cagccggaga gaatgggcag cgcaagaatg gaaaacataa agatgcattg cacacgatag
     1501 atattcttca tttcattctc ctttaggact cgccttgcct ctcagcaacg cgtccggatg
     1561 acgttcaagg taatcggaaa gatagtgaat ggaacgggtt gccgctgaaa gctccctgag
     1621 catgctgcta agctcaaatc ttagatctga ataatcgttg agcgattctt ttgtctgcag
     1681 catggtatca ttaaaagaac ctaatgcatt gtgtgccccc ggagcattca ccatcttgtt
     1741 tataccgtcc attgcatgat ccactttcgc gaagatttcc ttgagcggca gattctcgat
     1801 tgttttcgcc agttcatcca taggagcctc aaccgttgga atctccaggt atttcttctc
     1861 aatccctgtt aatttgatcg gtttatcggg atagaagtcc agattgatca tcaactgtcc
     1921 cgtgacaaag ctttgcattt gaagttggcc cttcagaccc tttgcgatca ggggtttaag
     1981 aagggcacgt tttctcatta ttgcctggcc ctcttctgaa acatgaagtg ttcttgtatc
     2041 gatttcaaca tagacgggaa tgacggcggt cagatcctgt gtattgaggc gcagctggag
     2101 accggtcacc tctcctattt tcacccctcg gaagatgacc ggcgaaccca cattgagccc
     2161 tttcacagag ccatcgaaga ccatcacgaa tttactcaag gaagtaaata atttccctga
     2221 gccgaaaatg accactcccg ctacgatcag ggctatggca ccgatgacga atgcgccgat
     2281 aagcgtcttg tttgcttttt tactcatggt ccttcacctc acttagtgat atctcgttct
     2341 gcgctgaagt agaaatcatg gttgtttcat accttctccc cgggtgagga agctgatgac
     2401 tttggggtct tttgactcgg caaggagttt cttcggatcc ccctgcgcga tcatcgtttt
     2461 cgtatccgga tcaagaaaga ccgagttatt gcctacggca aagatacttg cgagctcatg
     2521 ggtcacaatg accacggtag caccgagact ctccctgagt tcaaggatca gctcatcgag
     2581 caaatgcgcg ctgatcggat cgagcccggc cgacggctcg tcaaagaaaa ggatatcggg
     2641 gtccaatgcc atggcgcggg cgagtcctgc acgtttcttc atccctccgc tgatttccga
     2701 tggataataa tcatcgaacc cggagagccc cacaagggcc aacttcagcg agatgatctc
     2761 tttgatctgc cccggtttga gatccgtata ctgctgaagg ggtaatgcta tgttttcgcc
     2821 cagagtcagg gaactccaga gggcaccact ctgatagagt atcccaaatg acctcatgat
     2881 gcgttcctgc tccttcggct ctgcatccca gaagctgaca tccccataga ggaccctgcc
     2941 tttcgcgggc gtctgaagcc ccacaaggtg gcgaagcagg gtgctctttc cacaaccgct
     3001 tccgcccatg acgatgaaga tgtctccctt gttgatggtg aacgaaagat ccctctggat
     3061 cagaaaatca ccataggcca tggtgagtcc ttccacaatg atgtggggct ctttcttctc
     3121 tacggttttc ttcaccagct catccatgtt cacctatata tgtaacacgt tgcacaccac
     3181 agtgattagc gccattgcga ctacgatgct gacaatgctg gtgaccaccg cagaggtggt
     3241 cgcttcaccc actgcagagg cacttcgtcc gcactgcatc ccccgcatgc aacctgccaa
     3301 tgctacgagc acgccaaaga ccgcggcact gaagacccct acccataagt cgttcatgct
     3361 cactgattcc cttgtcttaa tgaagtattc catgaaattg aggtcaagca tggagacccc
     3421 gattatcagg ccgcccagaa cgcccatcag gtcggcataa aggctcaaca gaggcatcat
     3481 gaaaaaaagg gcgagcatcc tcggaagaac cagaaattcc atgggcgaga caccaagggt
     3541 cttcagggca tcaatctctt cgttcacctg catcgtgcca agctgggcgg caaaggcggc
     3601 tccggtcctt cctgccatga tgatcccggt catgactgct gcaagcgccc ggaccatccc
     3661 tatgcccacc aggctcgcga catagatctg tgctccgaag atcttcagct gaattgcccc
     3721 gataaaggca agtatcaacc caacaagaag actgatgagg gatacaatgg gaagtgcctg
     3781 tgcaccacat tcctggatag taagaaaaag gtcagaggga cggaacttgg cctttcctgt
     3841 aaacactctc acgaaggcga cggaggcttc tccgatgaat tccagcattt cgagaataga
     3901 cctccagaaa gcgatggcag agtcccctac cttctcgaca aaagatatcc tttccgcctc
     3961 cctacgagca cccttccttt caggaacggc agtagcaagg gagagaagcc gttgcacccc
     4021 tttgggaagg ccatcttttt ccaccgcaat atcagcagca gaacagagat ccatcaccct
     4081 gataaggaat gtcaataaac cgctgtccca ccccttgaga tccccagcgt caaaggctat
     4141 ctgccgcacc ttctgccctg ccctcatctc cttcatcacc tcatcagcag tcgggagaga
     4201 attcttgagc ttccactccc cagttatgcg gacaaggacc ctgttttcgg aggggcgctc
     4261 gaaggtcaga cttctcactg tgctcacgga tttatctgtg gacatgctct cctttcaaat
     4321 agcttgttaa ccattgcctt accactgatc tttctcaacc atctccccgg aggcgagatt
     4381 tctccgaccc cttttttgag agccgcctgc agggatttct ctgccttctc gttctcaccc
     4441 agagcataag caatggatga gttcaccatt gccatgacag cctgaggctc gatatagatg
     4501 gttcggctga cgcccatatg gatatagggt atcaccttcg acgggagcta tctactgccc
     4561 tttagggtac ctgcccttta gggaaggtag ggaaggtgtc gagacgcaga gggaaagtcg
     4621 tagcaacact ggttaaccta caagtgcaat ctggattttt atcggatcga attggtgcag
     4681 gaagactttc agatcacctc ttgcatgttt ccgaacttaa tctatttctt tgcgacgacg
     4741 agattgccga tgactatagg gaatacatat taaccagatc gccttataaa atctgtttgt
     4801 tatttaccgt acttcgcaaa cgagggtaat cattattgtt tgtactctgc acacagttgc
     4861 ggcaatctga acctatcgac tcctgtgagc agttctttca gcgcctttga tcacaaaacc
     4921 gtttgtttgc gggctgaagt ccggtcacgt tgcccgaagc gcttcgacga tgctcatccg
     4981 ggaagccctc actgcgggca gcaggcctcc ggcaaagccc atgatgaggg aaaaggccag
     5041 ggacttccct gtgatatccg gagtcagggt aaagctgaag gcaagctctg caaaagtctg
     5101 ccagttcatt gtggagatgg tgataagctg catgaaggat gcgaagaaga tccctataca
     5161 tcctcctata aatccaagga aaagggactc caggacaaag gtggcgagga tgctcccctt
     5221 ctggaaaccg agtgcgcgga gggtgcctat ttcggtgaca cggttcgcca cggcagcgta
     5281 catggtaatc atggcgccga tcaccgcgcc cagggaaaat atgacggtga gcgtcatgcc
     5341 cagtatgttg aggaacttgg ccatgccctc tgactgctca gcataaaata ttttctcccg
     5401 cttcgcctcg aggctgagtc ggggatcacc ctcaatgcgc gtcttaaacc cttcaaattc
     5461 agtggagtcc ctcagcctga atatgacgga tgaatagacc ggacggcgga aggcctgcat
     5521 gagctgatct acatctcccc atatttcaga actgaatcct gtgttgcctg catcaaaaat
     5581 ccccaccacg gtccagtcac gcataccaaa cctcacggtt tccccgatgc cccctccctg
     5641 aaaacgcttt gcgatactac cccctgcgat cacctcggca gaacccggct ttggcatgcg
     5701 gccctcaacc agtctcacct ggggtctcag gagaagggaa ccagccccaa tgcccctgat
     5761 caccacattc gcaggcttgc tgcttcccct tttgggaaga ttgatgagca ccaccagttc
     5821 ttttgcgagg agtggctcac cgttttcccc tatggcaact tcaggctggg tttcgatgat
     5881 cgaggccttg agccgttcaa ccccgctctg cacctctgaa ttggctgcct tgcggattac
     5941 caccacattg tcgtaagagc ctgtatccac caatgtcttc ctgagcccct ctgacaacat
     6001 gagggtagca gcgaagacaa agaccaccag ggccataccc aacgcagtga ggactgtggt
     6061 aagtttgcgg gtaaggaggt ttctgatgct gtaggaatag gggataccca tctaacctat
     6121 cctccggaga ccttccacga tacggatagt cgcactccgc cacgtgggaa agaccgctgc
     6181 caccacacct attcccatgg cagcaaggag gtccaggtat atggtaaagg gtgacacgct
     6241 gaatacgggg aagtactggc tcagttcaac ctcgaaccag cgggctgcag gaaaggtgag
     6301 gagcgcccct atgattcctc ctgtcatggc gatcacgaga gactccccaa atacaatccc
     6361 cccaatgtgc catttcccga agccgatggt cttgaaggta gcatactcgg atatcctctc
     6421 cctcgcagtc atcgccatgg tgtttgctgc cacaaccatg ataatgacaa tgaccacata
     6481 cgatacgatc tgaatggcaa tcaggatggc ctcggtcatc gagataaacc cgagttgaaa
     6541 ggcccgctcg gtctcggtca tggtctctgc cagagaattt ttaaaggtcc tgtctatttc
     6601 caccgctacc tctggagcga gatccttatt cgtcaccccg atcatataga agccgacctg
     6661 atcagcccgc cggggcacgg tcttcttcat cgtctcattc agatagtccc agtggaacat
     6721 gagggcacgc tcttcggtgc tcttatccgc acctttataa atagcgcgca aaacgaattc
     6781 ccactggccc gggaaaatgg tccccttcag ggtaatgata tcgccgagtt tccatttgtg
     6841 cagctccgcc agcttggcgc ccacaatgca gcccttacgg tccctgagaa atgccgcctt
     6901 ctgatccggc gggagcacaa attccgggta gagctccaaa tagcttctgg gttccactgc
     6961 gtagttggcg aagaagtgtt tctcgtcagc atagataccg ccaaaccagt tcccgtagga
     7021 gatgctcttc actccccgta tctggaggat cctgtccttg tatgaaatgg ggagtgtgaa
     7081 gacaagggaa atggcgttcc gggtgacgag ccgggtggcg gaagatcgct ccaccccgag
     7141 ataccagagg cccacaaggg tgcggagcag accaaaggcg aggatagcta tggcgacgcc
     7201 caccacagta agggtagtgc ggagcttgtg gcgaaaggcg ttccggaaga gaagcttaag
     7261 aaagaacatt gttcaacaca cccttatcga ggttcctcac catatgggcc ttttctgctg
     7321 cgcgggggtc gtgggtcacc atgatgatag tcttgcctaa ctcatgcaca aggcgttcca
     7381 tgaggtccag aatctcacgg gcagagaccc tgtcgaggtc accagtgggt tcatcagcca
     7441 ccagtatcgt cggatccgcc acaacagccc gggcaatggc aacccgctgc tgctgccctc
     7501 ccgaaagttg ggagggataa tggtccatcc ggtccgacag gttcaccgca taaagggctg
     7561 tctccacgtg ctcccgtctc tctctcctcg aaagccctgt gaggaggaga gggagttcaa
     7621 cattttcaaa cgcggtcagt accgggataa gattatagaa ctggaagata aagcctacgt
     7681 gggaggcgcg ccaacgggcg agttccgtct ccgagaggga agtgatctcg acctcgccca
     7741 ccctgacact gccgctgtcc ggcttgtcaa tgccggcgat gagattcagc agcgtcgtct
     7801 tccctgaacc tgacggcccc atgagggcca ggaattcccc ttcctcaata tcaaaggtga
     7861 tatcattaag tacgctgatg acctggcttc cgcgacggta ggttttgttc aggtttttga
     7921 tctcgacaat gggctgcctt ggcacttcca tctacttctc tgctaccttc accttgtctc
     7981 cgcccttcac cttattcaag ggctttagca ccactttttc acctgccttc acccctccaa
     8041 gcacctccac catgtcgccg atctgggacc cgagggtgac aggtgtctcc accgcccgcc
     8101 cgtccttaat gagaaatacc gccttctttc cgttgcggtt cagtaccgcc gcaggattga
     8161 tggcagtgcg tggtttctgc tcttcggcag taaccggcct ctggagaaag gcgacctttg
     8221 cactcatctc cggcaatatg cggggatcct tttccagaaa gcggactttg accattaccg
     8281 tcgccttgga acggtcagca gtgggcacga tcatatgcac ctcaccgcgg aaccgcgatt
     8341 caggaagggc atcaagctgt atctcacacg gctgtcccgg tttcacctgc tcaaggtcag
     8401 attcagagac gtcagcctcg acctggaggg agttcatgtc agcgatggtg accaccgctg
     8461 cctttgcatt tgcagctgcg ccaaacgggg tgatgacgtc tcccacgtcc gcattctttg
     8521 tcagcaccac tgcatcaaag ggggcgcgta tcagggtata ttcaatggcc acttctgccg
     8581 cccgaagcgc ggcgacattt gcctttaccc cggcttcact tgccgcaaca cccgcaacgg
     8641 ccttttggta ccgagcctcc gcagtatcat agtcagcctt cgcgataaac cctttggcaa
     8701 ccaaatccct ggcccggtca tatgcaagag tggcatcatg gagttcagcc cgcgcctgtt
     8761 ccagactgga acgggcaaga ttgaggtttg ccactgcctg gtctcgcgaa gcgaccacat
     8821 ccctgttttc gaggcgggcg atgatcgcgt tcttttccac ccggctccct tcctccacat
     8881 aaagggcttc gaggttcccg gtcgctttgg atgctacaga agccttgcgc tgcgccacca
     8941 cgtacccact cgcattcaga agggtgaatg tccgtgaggg atacacctgc gtcacagtgg
     9001 ctacctcgac ctgaacagca ggagagaacg ctcccttaac aaaaagcagg gcaatgaggg
     9061 caacaaggag cattgcccct attttatgga acggtttccc cctcttgaga tgacgtgcag
     9121 cgggagattt gtctatcttc aattttgaaa ggtcttcgcc tgccatcaat caaagcccct
     9181 gggttcaatt actccgggga aaatacgtct cattataaca gatttcccca tgtgcacatg
     9241 ccgactaaga aaatgaggag gtaagaataa ttccagggtt aaggaagtag tttatccttc
     9301 cgcgacacac aggggtcttc accggagggg tgtatgtctt acttctcggg gaattcggcg
     9361 acaccgtatt tcttcagctt cagataaagg gtcttcctgt ctatgccgag gagctggctc
     9421 gcctttgact ggttgccgcg gctttccctg agcactttga ggatatgctc cttctccata
     9481 tgctccagtg aagcatgcat ccccgtttct ttgactgaag agaggagggg aatcgagagg
     9541 tcatgggaat gtatggtatc ggtatcgcac agggtcaccg cccgttcgac cacattttcc
     9601 agttctctca cgttgccggg ccagtgatag ctgtggagcc cctccaatgc ctcagggcta
     9661 aaccccgaaa tggtttttga gagtttccgg ctgtatcttt tcagaaagtg gcgagaaagt
     9721 tcggcaatgt cttccctcct ctccctgaga ggggggatgg tgatgttgat cacattcagg
     9781 cggtaataca ggtcctgcct gaaccctcct gcagcaacac tttccgcaag attcttgttg
     9841 gtagcggtaa tgacccttac gtcaaccttg agcgtcttgt tccctcctag tcttctgaac
     9901 tcccccgaat cgaggaaccg taggagcttt gcctggagac cccgcggtat ctcaccgatt
     9961 tcatcgagaa aaagcgtccc cttgtctgcc acttcaaaga ggccgtactt tgcctggtac
    10021 gcagtggagt gggagccttt ttcgtaccca aagagctctg actcgagaaa gttctctgac
    10081 agtgtcgcac agttcagtgc gataagggga aacatgcttc gcttgctgtg ctgccatatg
    10141 gtattcgcaa cgagctcctt tccggttccg ctctccccct gaatgaagac cgtggaatcc
    10201 gtgggcgcga tcttcctgat aagcgcaaga atatgggtga cctggctgct cccgccaaca
    10261 aaatcaaccg ctgtttcttt tctgaggagc tcctgctgga actgctcgtt ctttatcttg
    10321 agcttcccgt attcataggc cctgttgata agaatcacga gctcatcaag cttatagggt
    10381 tttgtgaggt aatcataggc gccgtgtttc attgcttcca ccgcggtctc tacagtcgct
    10441 ttgcctgtca atacgatgat aaccggggga gcaggatcag cctttaattt cttcatgagt
    10501 gaaatgccgt taagtcctgg cataagaatg tccaggagca tcacatcaaa aaagctttcc
    10561 ttaaggagcc ggagagcggt ctcagcgtcc ggcgccacct cggcaaaaaa ccccttcctg
    10621 gcgagttctt tcttgaggag ccttctgaag ggctcctcgt cgtcaaccgc gagtaccctg
    10681 atcatgccgt ctttgcggga agatgaatgg taaaggtggt tcccctgccc gcttcacttt
    10741 ccgcatagat attcccgcta tgctcttcca caatcttatg gcagatggcg agccccagtc
    10801 cggtgccctc acccacaggc tttgtggtga aaaaaggatc aaatatctta tcaatgatat
    10861 cgtgaagaat tcccggaccg gtgtctgaaa tggatacctc aatcatttcc gcggtctcgc
    10921 cccggtgatc agacgcggat atgcctggtt gtgtggtaat gatgatactt ccaccctcag
    10981 gcgtgaaata gagagaattg ataatgatgt tcatgaaaag ctgcctcagg cttccggggt
    11041 ccgcgcttat cttcggcaga tcacggctga gtctgaactc aatatcatga ttgaattttg
    11101 ttgccttatg gttcacaagg agtatcacct cttttatgag ctcatttata tccaactcgc
    11161 ggaaggttcc cccatggggt cgtgcaaatt caaggagggt gttcaaaatc ctcttacacc
    11221 tgaatatctc attatgaatt gtctccagat actcgggaaa gtcctcaaac tccttcatgc
    11281 gtaaaaggga tatgtccttg gcccgttcaa ggagcgcttc cgagtagcct gcgataatac
    11341 cgagggggtt attgatttcg tgcgcaatgc ctgcaacaag ggtgccgagg gacgcaagtt
    11401 tgtctgcccg tatgagctgt tgctcgaggc gcttttgtgc ggtcacgtcc gtcaggtaac
    11461 gcacaatggc atacagctgt ccttcatcgt cagtgagggg ataggcccag aagtggaaga
    11521 gggtcccgcc gctgcccttc agctccgcaa aggacggtct ttccatggag agggcatccc
    11581 gcgtgcagct ttccacgggg ttgataccga ttctttccag gacctcgccg cagtgttttc
    11641 ccacgacctc gacgagggaa acaccggacc ttgcgaggag ggcgttgttt gcgcgaatga
    11701 cgcgctgcct gccgtcttca atccatacca tatccgtaat ggcgtcaaag gtctgctccc
    11761 attccttctt ctccctggaa atctcttcaa aaagaaggga gttttcaagg gcaatggata
    11821 tatgcttgga aaccgagagc aagagatgga ggtccctttc agagtattgt gaaggccttg
    11881 tgctgtcgag attgaatacc ccgaggatct tatcctggac aaggggaata ctgatggtag
    11941 accgtatgcc ttcatcgagg agtttcttgt ccagagtgaa cgcaatccct ttctcgagat
    12001 catcgtttat ccatggccgg ttgttcttga tcacccatcc ggcacttatg ccgtcaagag
    12061 gtgccctcac cccccttctc aatattgtcc tcatattggt atccagtgca aagatgagga
    12121 gattgttctc cttttcatta tagagcagga tgctgcccct gtcataatcg atgagcttct
    12181 tgatctctga catcatgatc ctgaagaccg tgcgcatact caggcttgaa ttgatcatac
    12241 tgctcagttc attgatgagc ttgatttcct tcatcctgtc cttcagctgt cctatgagct
    12301 ttgtattttc aatagcgaca cccaattcac ttgcgatcgt ggtgaggagc tgagtatcca
    12361 gagaagtgaa ataggattcc ttcctgctcg caagtccgag aactcccagc ggcgttcctt
    12421 tcgaggtgat gggtaccccc gccatgctct gcattcccgc gcctttgtga agcacccttg
    12481 ggtcttcgga cgcactttcc actatcaggg gtctgccgga cgagaagacc ttgccgatga
    12541 agccctcccc agccttgcgg caggagattt cgttgagaag ggttttgggg agcccctgat
    12601 gggcaacaag acggagttct ccggaggcat cgtccatgag gtaaacccat ccattgtccg
    12661 cctccataag gtaacgcagc atatagaccg tctcctggat gattttccct gcacccgtgt
    12721 atcgcgcaag ggtcttcgca aagttattaa acaccgcgag ctcctttgcc cgctgggatg
    12781 ccctctgctg gagctttttc tggacagaca tgtcctgcac ggtcacaatc tgcagatcat
    12841 cgtgaggata ggggacaaat gatattcttg caggaaaaga cttaccattt ttctttttgc
    12901 agagcgattc gaactggctt cccccctcct ctttcccgct ccatgaggga gggaacgact
    12961 ggggaaagac gaactcagag acctcccggc cgctcatact gtcaggcatg acctcaaaaa
    13021 tggtatttgc cgcagtattc gccaaaacaa cgcggcgctg tgtatcgatc ataatggcag
    13081 ggatgttcag gaaatcaaaa acctcgagga taaagccggg cttctcggta agttccgctc
    13141 tcattgctgt tcatgtatta tatcataaga ggaaaagagg cttccctgac cattggggat
    13201 gaacgacctt tttcaccgga gggaatgaga cagtgaccga aaaggacctg agtttttttc
    13261 aatcatggtt ttcccgatat tgcaggtctt tttataccgg caatgaggag gaccggagaa
    13321 atctgctcct taaaaaagac catacccgtc atgtgtgcga gaacatgcga cgtatcgcaa
    13381 aggacctctc cctggagagc gccgaagaaa tgctggccga aaccatcgcc ctcttccatg
    13441 atgtcggaag atttccgcag tacgcggaat ataagacctt cagggacagc atttccgtca
    13501 atcatgccac tttgggaaca gaggtcctct cacgggaggg tgtgctggag accctcccga
    13561 agaatgaaca gcggataatt cttcaggcca taaaattcca caatgcatat gccgttccgg
    13621 acattcggga taacgatatg ttactctttc tgaaactcat acgggatgcg gacaagcttg
    13681 acatctggcg tgtatttatc gaatacttgg cattgccgga aaaggagagg gcatctgctg
    13741 tggggcttgg attgcctgat gtccctgagt attccgagaa tgtcctttcc tgtgtactaa
    13801 gcaaagaggt cgcctccctt tcaaacctga agacactcaa tgatttcatc cttatgcagc
    13861 tctcgtgggt gtatgacctc aatttcagaa catcgttcat cctgttagag gaaaggaatt
    13921 atataaacaa actcatcagc accctcccct ccacagaagc cataggcaag ctctctccct
    13981 tcctggggga atacgtcagg gaacacattc aagcggctcc ttcgcaccac gacctatgag
    14041 cagaaggcgc ctgggacaga acttcctttt tgacccttcc atcttaaaca gaatgataga
    14101 gatcgcgcgc ctttcgccag aggacaccgt ggtggagata ggccctggtc acggaagact
    14161 gaccgccatg ctcgcaaaaa aggtcaaaag ggtcatcgcc atcgaacttg acgcgacgct
    14221 ctacgagaga ctgcgcgaga cactattagc atacgacaat atcgaggtcg ttcatgccga
    14281 tgccctccat tacccatatg acaccctcga tacattcaaa gtggttgcca acatacccta
    14341 ctacatcacc acacccatca tcttcaggct ctttgagcac agaaatcgcc tctcttcaat
    14401 caccgtaaca gtgcagagag aagtggcgcg caggattgtt gcgggacccg ggggcaagga
    14461 ctatggcgtt ctttccctga tggtccagta ttatgggcaa ccgaaactgg aatttgttgt
    14521 acctaagggc gccttcaggc ctgtgccgaa ggttgattca gcagtagttc atattgaact
    14581 ccacgaaaag cccccggtcg ctgtgagaga cgaaaggttt ttcttcagag cgataaagac
    14641 cgctttttcg cagaggagaa agatgctcct caacgccttg aagcctatct ccggggatat
    14701 aaaagaaaga cttgtctttg ctggaatcga tccaaccagg agaccggaga cattaagtat
    14761 ggaagagttt gccaggctct ctgatgaact gttaagcaaa cacagagact cttcagagtc
    14821 cggttaattc atctgaccat caagaatccc atgaggcgtc gcaaggcgga gtttgtgttt
    14881 tgagtttttt aagcaacgaa ggagtaagca ttgtcttcat ctgaaaatta tcctctgcga
    14941 actcatccct ctttttcatc agaagccatt ccttttcctt gcctgacatc cttgccagta
    15001 tgaagatgcc cttcagtctc tttcccttaa gggagaattc cagcctgccc atcctgatat
    15061 ttcccccgag atatccgtat tcgccgctgt cccaaatgag caccgcgcct gccccataat
    15121 tgccttcagg aatgattccc tcataggagc catactcaag ggtgtggtcg tccaccataa
    15181 tggcgagcct cttgtctttc gggttcatgg atggcccctt gggaacagcc caggacttga
    15241 gaaccccctc catctcgatc ctgaaatcga aatggagatg gctcgcatga tgttcatgga
    15301 ccacaaactt tggcatctta cttttttgct gtctgctcag acaataggat cgtcattcct
    15361 aagaatatag tgtatcaggc ttatggacag ggtgcaaaac tttttcttat ttcactgaat
    15421 gggagcaatc tcgccttctc acagaactcc gcagagccga gcgtcccaat cgttctgtag
    15481 gtttcgagtg aaaatcgtta catgtgccat ctgaaactcg cctttagcat tcctagatag
    15541 gccgagctcc agcaagcctt gcctaggcct cttcttttct tcgccgtgta tttatggttg
    15601 cacaaccagg atcttttgta caatctcggg atagtttgcg atgaaccgca gttcatcatc
    15661 agtcagaggt ttctgcgtgt ggaggtttgc atactggaca agctcgagaa ttcttcttgc
    15721 ctcgctgtca ttgtggaagc tgattcctag tttgtcgtac gcatgcctga atccctttag
    15781 gccaccgtat tctcctgtcg tgatgatcct tccggtttcg agcaattcgg gttcacctct
    15841 ccccacatct tctggatcat agagttcata actccgcctg tccttcaggg caccatctgc
    15901 atggatcccg gattcatggg caaatgcatt ggcacccaca cccggctgtt ttatgggtat
    15961 ggggatattg aatgcataag atgcatactt tgctattttc caggctgtct ttagattgat
    16021 gtgttcatcc aaggctatct tagcctttaa tgcatgggaa aacttaagtg cgaggattgt
    16081 ggataccaag tcgcaattcc ctgcccgctc cccgtagcca ttaattgttg tgttgatgta
    16141 ggagtcaaca ttgctctcga atgccccctg ggcgcctgcc acagaaacag ctcctgccat
    16201 gccaaggtca ttgtggcaat gcatttcgat aggaacttta gtagccaacg ccagtgcctt
    16261 tattcgctca tatatagtaa ttgggtcatc aaccccaagg gtatcgcagt acctcattcg
    16321 gtctgctccg gcctctttcg ctgccaatgc aaattcgatg agtttgtcca ggtcagtcct
    16381 agaagcgtcc tccgcattca cccccagcgt cactaccccc aactgctttg cgagttttgc
    16441 agacttatac atcgattcca caacatcttt ccatgtcttt ctgccctgga atttcccctg
    16501 tatcataata ggcgatgtgg ataccgagat attgagatgg cgcagaccgg ggcagttttt
    16561 aaaggaaagc tcgacatctt caggcaatgc cctgcaccat ccctcaagat gcagtctctt
    16621 gataccacct gccttgacca aatcaagatt tgcattgata tagttaattt catgtttaag
    16681 cgtggggaat cctatttcac tctgataaac ccccatttcg tcaagataca ggttcagcat
    16741 cgtttttgac agtttcggaa gtaaaatcct tgacgtctgg acgccgtccc tgttcgtcac
    16801 atcgatgaga tagagtgctg tgttcatggt gtcctgttca gttcccagcg caagggatca
    16861 gcccaaatgg ctgcaggctc ctgcgttctg taggcactgc tttctacaag gtccatcagc
    16921 tcctgggatt ccggcaaatg gatgatatca tctggtctgc cggattgaat aatttttccc
    16981 gctgagtata cgaacatcct gtccgccagt gcgcgtgcct catgcagatc atgggtaacc
    17041 aaaacaaccg gtatgttgaa ttcatgctgc acttccttca gcaaccttcc cattcccagc
    17101 ctaatagaag catcaagcgc ggagaacggc tcatcaagca aaagcacacg gggttttctc
    17161 atgagagctc gcgctagcgc gacccgttgc ttctgacctc ctgatatcct tccggggagc
    17221 ctgtcccgta actcggcgat gcggaaacgt ctcatcattt caacagccat actttctttc
    17281 tcagtctttg atatgccgta gcccccgtac aggatgttct ggtaaaccgt catatgggga
    17341 aataacgcca gatcctgaaa cacgtagccg agatgccgat cctgcggttt cagtcgtgtg
    17401 cctgctttac tctcaaaaaa taccacatca ccaagccgta tttttccctc atccggctca
    17461 acaaggcctg cgatcatctg gagcgtaagg gacttgcccg cacctgaatg accaaacagc
    17521 acggcaagtt cattattcat ctgccacgaa acgtcaagga cgaaactgtc catttttttt
    17581 cgtattttga ccgataggga catcagatca acctcctgcc gaaccggttt gcgatataaa
    17641 ggaggagtcc ggatatgacc gtaagaagta tcaccatagc gtttgctttg gaccattccc
    17701 cgctgcctgc aattgaataa attgcgagtg gcatggtgtc ggtcttccca ggaatgttgc
    17761 cagcaaggat c
//
LOCUS       DATKOU010000117        23017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3235590, whole genome shotgun sequence.
ACCESSION   DATKOU010000117 DATKOU010000000
VERSION     DATKOU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 23017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23017
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235590"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..778)
                     /gene="thiE"
                     /locus_tag="VMU21_05385"
     CDS             complement(149..778)
                     /gene="thiE"
                     /locus_tag="VMU21_05385"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HUO76994.1"
                     /translation="MRERLIDFNLYLITGRKLLAAHHSLFSAVEDALKAGVRAVQLRE
                     RDLPIRELLEMAYEMRELTGKYRARLFINDRVDIAVCVEADGVHLGQSGIPVSAVKKM
                     VKEGFIIGASTHSVEEATTAEREGADFITFGPLFETPSKIKYGVPLGIDALREVRERV
                     SIPFFGIGGIKPGNVREVMNSGAFGIALISGILAEADIKGAVERYLNIM"
     gene            complement(807..1577)
                     /locus_tag="VMU21_05390"
     CDS             complement(807..1577)
                     /locus_tag="VMU21_05390"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HUO76995.1"
                     /translation="MEDTLIIRGIEFRSRLWIGTGKYRDFEETARAVEASGADVVTVA
                     VRRVNITDRKSENLLDFLDPKQYKILPNTAGCYTVEDALRYSRLAREAGVSDMIKLEV
                     IGDEKTLFPDTIGLLKATEILAKEGFMVFPYTNDDPIMARRLEDAGAAAVMPLGAPIG
                     SGLGLRNPYNIRIILETAKVPVIVDAGVGTASDATIAMELGCDAVLINTAIAGAKDPI
                     AMAEAMKHAVIGGRLAYRAGRIAKKLYATASSPLEGML"
     gene            complement(1603..1854)
                     /gene="thiS"
                     /locus_tag="VMU21_05395"
     CDS             complement(1603..1854)
                     /gene="thiS"
                     /locus_tag="VMU21_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HUO76996.1"
                     /translation="MFLGKNEMRLTVNGEAYCTEKNTVRELLGEMDIVPERVAVEINL
                     KVLKRAEYTEYKLQEGDSIEVVYFVGGGGGAQRVPAISA"
     regulatory      complement(1894..2001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2160..3842
                     /gene="mutL"
                     /locus_tag="VMU21_05400"
     CDS             2160..3842
                     /gene="mutL"
                     /locus_tag="VMU21_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HUO76997.1"
                     /translation="MAEIKILPVEVRNKIAAGEMIERPASVVKELMENSIDALSTHIR
                     IEVLRGGKGLIKVSDNGRGMDRDDALLCLERHATSKLSSANDLFSISTMGFRGEALPS
                     IASVSRMSLVTGLQDAPSGVFVEAHGGEVKDIRDFPAQGTSVEIRDLFFNTPARKKFL
                     KSDRTELFHIIDWVTREALAHWDIGFTLFTEKQETMSIPRASDPRERILQIYGLEFVE
                     ELLELHAKDPGLAITAFVTKGEHFRSSKSHQLIFINKRPVKDQTISHAVYQAYEGLLP
                     HDRHPVFFLFMDIDPRRIDVNVHPTKREVRFENKDLIHRFVSSTVREGIKEERSHYTA
                     PFIEPSMHAFPGPGVKVPSPGYREPVMRERGVSENCDLLYQPSLPFLYLGDTFIAMAG
                     KGGLTLIDHHAAHERILYENFLKGMNLNSYPLLFPRQIKLSHKEYALLLENKDTLHEM
                     GLEMDDFGHDTLVVRSLPEALEEADLRGILSDVAAALGEGIRPFSRMKEVLAARIACH
                     SSVRGKDILHQEEFHQLLQRLEDTEHPDQCPHGRPTRIFLSLDDLKKMFKRK"
     gene            3861..4781
                     /gene="miaA"
                     /locus_tag="VMU21_05405"
     CDS             3861..4781
                     /gene="miaA"
                     /locus_tag="VMU21_05405"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HUO76998.1"
                     /translation="MKKVIILLGPTGVGKTGASILLAQSLNTEIISADSMQIYRHMDI
                     GTAKPSKQEMSLVKHHMIDIVEPSETFSAGKYIEHVTPIVEGLHYQGKIPVVVGGTGL
                     YIKAMTRGLFSGPSADWHLREELLDREEEKKGSLYASLMKLDPSAAARIMENDTRRIV
                     RALEVCLKAGRGITQLQESLTTPLPYDFIRIGITRERKELYGLIEKRVDSMLTAGLVD
                     EVRSLLLRNPEKTALQAIGYKEIAAHLRGEYPLDEGVRLIKKRSKNYAKRQITWFKRE
                     EGMRWVDVTGHFTGKEIYEAIAIFIENELP"
     gene            4838..5056
                     /gene="hfq"
                     /locus_tag="VMU21_05410"
     CDS             4838..5056
                     /gene="hfq"
                     /locus_tag="VMU21_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HUO76999.1"
                     /translation="MSNSLQDNYLNQLRKDKTPVVVYLANGVRLKGMIKAFDNFVIVL
                     KESTQQLIYKHAVSTIVPEKDVDLRFEE"
     gene            5031..5444
                     /locus_tag="VMU21_05415"
     CDS             5031..5444
                     /locus_tag="VMU21_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUO77000.1"
                     /translation="MSTCGSKNKKNPFPTVDLIIECEGGIILIKRENPPLGWALPGGF
                     VDYGESLESAALREAKEETGLEVALVRQFHTYSDPHRDPRHHTITTVYIAKAKGRPVA
                     GDDAAEAMLFSQATLPESIAFDHKSILDDYFTKRY"
     gene            5529..6194
                     /locus_tag="VMU21_05420"
     CDS             5529..6194
                     /locus_tag="VMU21_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HUO77001.1"
                     /translation="MLRAMRKHAKYFYVLFFIVILSFIFWGVGTMDKSTGVPVAEVGK
                     ERITVEEYWTSYDRAREFYRTLSQGNFTEEAEKKLNLKQRVLDSLVDDLVLMTEAKKA
                     GIRVSDGELADAIVNEPAFVRDGTFNKEIYVRTLQLNRMTPEMFESMKRRELTLARMR
                     KLIGESVDVLVSDVPQASGNEQALASLRQTALPEMRDRAVKSYIEGLKQQMKIKVNQQ
                     LIS"
     gene            complement(6274..7662)
                     /gene="selA"
                     /locus_tag="VMU21_05425"
     CDS             complement(6274..7662)
                     /gene="selA"
                     /locus_tag="VMU21_05425"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HUO77002.1"
                     /translation="MDTRDLLSSLPSVDEALKSGYGREWLEKYPRRYVLDAIRETISL
                     RRKEILEGTTKEVSLASMAPDISLNVKRLATPSLRRLINATGIVVHTNLGRSILSERI
                     MENVSTIATGYSNLEYDLASGSRGKRHTHIKRLLREITGAEDGFAVNNNAAAVFLCLT
                     ALAKGREVIVSRGELVEIGGSFRIPDVMSHSGALLREVGTTNKTHLHDYEHAMSDATA
                     LILKVHRSNYQIVGFTEEVPVEKLVSLGQGRGVPVMYDLGSGYLIPLHQYGIHGEPAV
                     QEMVRSGADLITFSGDKLLGGPQAGIIVGKRELIEKIQKHPLARAVRIDKLTLAALEA
                     TLMEYVDGAQAKEHIPTLRMLLEDPGTIKTRAKKIASLIKREIKDAVLQVLEDSSQAG
                     GGSLPEVEFPTYVVAIRPENISVNELEERLRKGEPAIIARIQGNSLILDARTIGDQEI
                     TAVAHAVTAALR"
     gene            complement(7670..8149)
                     /locus_tag="VMU21_05430"
     CDS             complement(7670..8149)
                     /locus_tag="VMU21_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUO77003.1"
                     /translation="MEMERGDPGELKESLTGYKEKVAPVTKGTLTWDKDLIFVGRTQR
                     GYEIEFDAEIQWGCAPTESLLLSLAACMGIDAVSFLQKMRANLTSFKIDITGERNPTP
                     PQYYKAIEMVLHISGERITQKQVERAVALSQEKYCSVYHSLRKDLKVSVKYILNGQG"
     gene            8252..10126
                     /gene="selB"
                     /locus_tag="VMU21_05435"
     CDS             8252..10126
                     /gene="selB"
                     /locus_tag="VMU21_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HUO77004.1"
                     /translation="MRYVILGTAGHIDHGKSSLVKALTGIDPDRLKEEKERGITIDLG
                     FADLSYPDGLTVGIVDVPGHERLVRNMLAGAGGIDLVLLVIAADEGIMPQSREHLAIC
                     NLLRIKTGLIALTKTDLVEKEWLELVKDEVRSFVRGSFLENAEIIPVSSKTGDNLELL
                     KERIRDVALTVEQKPVREIFRLPIDRVFTLKGFGTVVTGTVISGTLSLDDMVEIEPSK
                     IHTKVRGLHSHGKAIGTAYAGQRVAINLQGVNKEDLKRGDVVVTPRVVSASRIMDAKI
                     ELLHDVPPLKSKSLVHLHITTSESIARVILYNREELRPGESCYCQLRLEEPAVALSGD
                     RYIVRKFSPVITIGGGTILDPSPLRRRRKEALEDLDVLEQGALPERIAVKVKKSGIKG
                     MKALSLSGWIKAETHSIDESLSLLKKQGGLKQLGETLLHRDIYTALAKGITETLSSFH
                     KKNPLSPGMLKEELRAQLKVGPELWNTLLSSLEAVLVDKDVVRMKDAIIASSVASQSS
                     EKILQLLEKARFQPPSREELSESLKMDPKALGDLLKLMSKEGSVVRINDSLYVAGTAF
                     KEMISLLRGFFAKKPAMTVAEFRDILDTTRKYALPFLEYLDSNNITLRVGDVRKFLLK
                     "
     gene            complement(10185..10260)
                     /locus_tag="VMU21_05440"
     tRNA            complement(10185..10260)
                     /locus_tag="VMU21_05440"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10225..10227),aa:Phe,seq:gaa)
     gene            10311..11864
                     /gene="lnt"
                     /locus_tag="VMU21_05445"
     CDS             10311..11864
                     /gene="lnt"
                     /locus_tag="VMU21_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HUO77005.1"
                     /translation="MNTQTKGGMPLKLLLRTYLPAAISGLLLFASFPTIDLFPLAWIA
                     LVPLMLSLWDKGPRAAFFSGLLTGTLYFFGTLYWIYHSITYYGSVPFIISVVLVLLLS
                     LYLGLYVGLFALVFSLKIRHTRLPALILAPVFWVTLEFIRSYALTGFPWASIGYSQYR
                     FLYGIQYADITGVYGVSFLVLAVNSAIADFFILKRRRAEMPLYSVSYTFISYAVFFLL
                     LLTLFPYSYWRIHQQRPGNPVRVSIIQGNIEQDKKWQPGYQDEVMRIYKELTTKATAS
                     TPSLVIWPETSVPFYFGYDTARSRDLVEFQRRINSYLLFGSVMVKSPPAKRQPVQLTN
                     SAVLLDPEGNISYVYDKIHLVPFGEYVPLRSIFFFLDKLVAGIGDYEPGNRVIKANTG
                     IGSFGTFICYEIIFPGLVRKCFSKDGDFMVTITNDAWFGRTSGPFQHFSMAVFRAIEN
                     RKPVLRAANTGISGYIDSNGKVLMSTSLFERREETVEMTTDSSRSFYSKYGDIFSYIC
                     IVVSLLLLI"
     gene            11878..12991
                     /gene="prfB"
                     /locus_tag="VMU21_05450"
     CDS             join(11878..11952,11954..12991)
                     /gene="prfB"
                     /locus_tag="VMU21_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006077.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HUO77006.1"
                     /translation="MLRIDEIRQDLVSLRVKIETLRGYLDVPHLKEDIVRLEGELSLE
                     SSWTSPETLHVVQKKKAALEGVVFPFDAAAERLSYLEDSLDILEEEGGDAFLKEIQEG
                     IAELRESIERIELRHFLSGELDLNNAIVTIHPGAGGTESQDWAQILMRMYFRWAETNG
                     FTTDVIDLQPGEEAGIKSVTFTVTGQYAYGYLKAEAGVHRLVRISPFDANKRRHTSFS
                     AVLVFPEIEENIKVDINDDDLKIDTFRASGAGGQHVNKTSSAVRITHIPTGIVVTCQN
                     ERSQHKNKGTALKILRARLYDLQQEAQERKVEGIVGDKKGISWGNQIRSYVLQPYRLV
                     KDHRTGLEVGNVDSVLNGNINSFIDAYLREKGKVVQ"
     gene            13319..14650
                     /gene="dnaA"
                     /locus_tag="VMU21_05455"
     CDS             13319..14650
                     /gene="dnaA"
                     /locus_tag="VMU21_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HUO77007.1"
                     /translation="MPLEAIWNKSLTIIEKKVGENAFELWFKPIKLSQLKDQSAVIEI
                     PNRFFKDWVEDYYPTLINDTMEEIVGHPITVKFKIAEKEDSEVKRIDARLEGRKQRLA
                     SKGIYLNPKYTFSNFITGQSNQFAHAAAMAVAESLGKAYNPLFIYGGVGLGKTHLITA
                     IGNKVADKRQDFNILYVSSEQFTNEVVSAVRHGKTEELKGKYRNLDLLLVDDVQFIEN
                     KTATQEELFHTLNTLYEKQKQIVLSSDRPPKEIKNITDRLRSRFSMGLIADIQPPEIE
                     LRMAIIQKKAATERIQLSDEVVNFLASRVKSNIRELEGSLIRLGAQSSLTSEPIDVSM
                     AKNILKDIISEVDRPITIEQIQKSAAEFYGIKYQEMKSKKRTKEIAIPRQVAMYISKQ
                     LTGSSLSDIGKNFGGKDHATVIYACKQIEGKREKDEEFNRKVESLIKRIQG"
     gene            14664..15764
                     /gene="dnaN"
                     /locus_tag="VMU21_05460"
     CDS             14664..15764
                     /gene="dnaN"
                     /locus_tag="VMU21_05460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HUO77008.1"
                     /translation="MKLTIAKDEIQEKLSNIQNIVEKRNTMPILSHFLLDAGKKETHI
                     VATDLQIAIKEPLKANVQSEGKLCIPARKLFEIVREAEGDITIESEDEQWIKVSFGKS
                     RFRLACLASAEFPAWPGIETGEEVKLGIDTLSEMIEKTIYAAGETDTRYTLNGLLFHI
                     RPADTTLTLVGTDGHRLALLSKGLESKAEEETKVIVPRKAAAELRRFFGSSESVSAVM
                     GKNHLLFRIGDVQFLVRLIEGTYPNYEQVIPPSNEKKMTIHREGLLRTLRRVSIMSKE
                     RSNAVKVDITQGSLAISSSNPDLGEARDEISVEYAGEDLSVGFNARYLMDALGAMTAE
                     KVVFALQDSLSPTLLKEEGNDEYKCVVMPMRI"
     gene            15861..18284
                     /gene="gyrB"
                     /locus_tag="VMU21_05465"
     CDS             15861..18284
                     /gene="gyrB"
                     /locus_tag="VMU21_05465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HUO77009.1"
                     /translation="MEEKDKERNREKIEAEAEAYGAEDIKILKGLDAVRKRPAMYIGS
                     TGPDGLHHLVYEVVDNSVDEALAGFCNAIDVMLHHDGSCTVIDDGRGIPTAPHPGDPE
                     GRSAAEVVLTELHAGGKFESKAYKISGGLHGVGVSVVNALSEWLEVEIKHNGQVFQQR
                     FERGKPAGPLSVVGKTKGRGTKVTFKPDPEVFEMTDFSYDVLSQRLRELAFLNRGLKI
                     TILDERVDKKQEFLYKGGIVSFVEHLNKNKTALHPKPIYISGEKEEIFAEVALQYNDG
                     YAENIFTFANNINTKEGGTHLIGFKSALTRTANSYGSASGILKNGKETLSGDDIREGL
                     TAVVSVKLPNPQFEGQTKMKLGNSEVKGIVESIVNETLATYFGENPSIARKVIEKAIQ
                     ASRAREAARKARELTRRKGALEDTGLPGKLADCSEKDPALSELFIVEGESAGGSAKQG
                     RDRRTQAILPLRGKILNVEKARFDKMLGHEEIKILITALGTGIGADDFDIAKIRYHKV
                     ILMTDADVDGAHIRTLLLTFFYRQMPQVIENGYLYIAQPPLFKVKRGKTERYIQSEAE
                     LQNMLFELASDDLSLSIKGQEVKGKALIPFLKRLLNFERLLEWFERRRRDPLVLRIFV
                     AEGITKALMKDREKISTLLEKVTKQLPDANIGDLYEDEEHEGYGVEIRRQSYKLVLDT
                     NLLLSPEFRELETLYAFMKDLGKAPFKISTKDGQKEVASTTGLLALIVETARKGLSIQ
                     RYKGLGEMNPQQLWDTTMDSDKRTLLQVSIEDSVQADQIFTILMGDQVEPRKEFIVKH
                     ALEARNIDI"
     gene            18481..21306
                     /locus_tag="VMU21_05470"
     CDS             18481..21306
                     /locus_tag="VMU21_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HUO77010.1"
                     /translation="MGRTPEDEARQVIDGLLEKAGWHVCDYKEANIHSSTGVVIRNFP
                     LKTKHGIADYLFYIEGKAAGVIEAKKEGTTLTGVEIQSDKYKNGLPEILPSWHRPLPF
                     CYESTGVETRFTSYLDPDARSRQVFSLHRPETLHEWLTYSGEATLDKAADPLGIYWPP
                     STLRSRLRKMPQLIEGGLWPAQIKALMNLEKSMSETRPRAVVQMATGSGKTFTAVTFI
                     YRLLRFAGAKRVLFLVDRANLGRQTLKEFQQYQSHYNPYKFTEEYIVQQLTSNKIDKT
                     ARVCISTIQRLYSILKGEELPEEEDEHSTAGLEYVIKEPPPIEYNPNVPIETFDVIVT
                     DECHRSIYNLWRQVLEYFDAFLIGLTATPNKQTFGFFNQNLVMEYPHEQAVADGVNVN
                     YDVYKIRTKITEGGSKVDAGYIVDKRDRETRTVRWEKLDEDFAYTAEQLDRDVVAIDQ
                     IRTVVRAFKNKLFTEIFPGRTEVPKTLIYAKDDSHAEDIVKIVREEFNKGNEFCQKIT
                     YKTTGKKTDELIAEFRNSYFPRIAVTVDMIATGTDIKPLEIVFFLRQVQSRAYFEQMK
                     GRGVRVINETDLQSVTPDAKSKDHFVVVDAVGVCEAAKTDSRPMDRQRTVPLEKLLQA
                     VALGSTDTDIISSLAARFARLERRITKEDNAEIRKFADGKGLKELTGSLVQSLDPDRH
                     IELARAEQSEAGGTGEPTEEQIKKAAEKLIQSGVTPLHNPRVREKILEIYKKTEQTID
                     TVSTDEVTTVGFSAEALEKAKGLVQSFEQFIKEHKDEITALQILYSKPYKTRLKYEQV
                     KELANLIEKPPYFWKVDNLWNAYAALEKSKVRGASAPHILTDLVSLVRFAMHQENELV
                     PFPERVNSNFSAWIAQQENAGKKITDEQRRWLEMIRDHIAGSLSIETDDFEYGKFIQQ
                     GGIGKAYQVFGDELSRMIEELNGVLAA"
     gene            21319..21645
                     /locus_tag="VMU21_05475"
     CDS             21319..21645
                     /locus_tag="VMU21_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77011.1"
                     /translation="MTKKNFWDKIGTETNTERSTAMNTLAAQEIKRKGISAVDEVLKE
                     GPVHIIKNNQPQYVVLTEERYRDLLEAEDEAYNARIRASLEDLKAGRVKRGTAKDLIK
                     ELGLED"
     gene            21649..21924
                     /locus_tag="VMU21_05480"
     CDS             21649..21924
                     /locus_tag="VMU21_05480"
                     /inference="COORDINATES: protein motif:HMM:NF027074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HUO77012.1"
                     /translation="MYALVWTAHFTRAAKKFGKSHPELKKKVAGILRDLEKDPFQPHL
                     KYHHLGGKYKGMQAISITDSYRITMTVMITEKEIILIDIGTHDEAYR"
     gene            21921..>23017
                     /locus_tag="VMU21_05485"
     CDS             21921..>23017
                     /locus_tag="VMU21_05485"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HUO77013.1"
                     /translation="MKPRSPVKACADSILSFSKDFMTKRNQPPLQEKASLDHEKQNNT
                     PLPDGWVWKTLGEVLSDIVGGGTPSKTNSKYWNGTIPWLTVKDMRTRRPADSIDHISK
                     DAIRESATNLIPKDTVIIATRVGLGKVIRVPYDATINQDLKALITTAEVDKGYLEHWI
                     SSIANYLESIGSGTTVKGIRLETLREIPFPLAPLDQQERIVSEIEKHFSRLDEAVASL
                     KRVKSNLKRYKAAVLKAAVEGKLTEEWLKAHPNVEPAGELLKRILAERKKKWEEKNPG
                     KKYKEPAAPDTSNLAALPAGWVWATVEQLAGKIQYGSSSKTNEDRQGVPVIRMGNIFE
                     GRLKLDDLKYLPRNHSEFPELFLKKNDLLFNT"
BASE COUNT         5977 a         5886 c         5936 g         5218 t
ORIGIN      
        1 gagcctgtgc ggggatatcc cttccgccag ggccactgcc tccatttcct tggtaataat
       61 ccccttcctc gcaagttcta ttctcgtcat aacggttctc ctcttcctct tttgtcaccc
      121 tctgtaaagg gggaacgaac ggaaattgtt acattatgtt caaatatctt tctacagcac
      181 cctttatgtc agcttctgcg agtatgccgc tgattaaggc gatcccaaaa gcccctgaat
      241 tcattacctc cctcacattt cccggtttta tgcccccgat tccaaaaaaa gggatagaga
      301 ccctttctct cacctctctc aatgcgtcta tccccaaagg caccccgtac ttaattttgg
      361 agggcgtttc aaacaaagga ccgaaggtga taaaatcagc gccctccctc tccgcggtgg
      421 tcgcctcctc cacactatga gtggaagccc ctatgataaa cccttctttc accatcttct
      481 tcaccgcaga cacaggaatg cccgattgcc cgagatgcac accatcagcc tccacgcata
      541 ctgcgatatc caccctgtca ttgataaaga gccttgcacg gtatttccct gtgagctccc
      601 tcatttcgta tgccatttcc agcagttccc ttataggcaa atccctttcc ctcaactgaa
      661 ccgccctcac tcctgccttg agcgcatctt caaccgcact gaagagcgag tggtgcgcgg
      721 ccaacaattt tctcccagtg atcagataaa ggttaaagtc aatcaaccgc tccctcatcc
      781 ctcttgcgac ccaccgttca ctggagctac agcattccct ccagggggct gctcgctgtt
      841 gcgtagagct tcttcgctat tctccccgcc ctatacgcaa gcctccctcc aattaccgcg
      901 tgtttcatag cctctgccat ggcgatgggg tctttcgctc ctgctatggc ggtattgatc
      961 agtaccgcgt cacagccgag ttccatggct atcgtggcat ccgaggcagt ccctacccct
     1021 gcatccacaa ttaccggcac cttcgctgtc tcaaggatta tcctgatgtt atagggattc
     1081 ctcagcccca gccccgaacc tatcggtgct cccaagggca tcaccgctgc ggcacccgca
     1141 tcttcaagcc tcctggccat gatggggtca tcattggtat aggggaacac cataaacccc
     1201 tctttagcca gtatttccgt tgcctttaat agtcctatgg tgtcggggaa cagcgtcttc
     1261 tcatctccaa tcacctctag ctttatcatg tccgataccc ctgcctcccg cgcgagacgg
     1321 gaatacctca gggcatcctc caccgtataa cagcctgcgg tgttgggaag tattttgtac
     1381 tgcttggggt ccagaaaatc cagaagattc tcgctcttcc tgtcagtgat attcaccctc
     1441 cgtacggcta cggtcaccac gtccgcccct gaggcctcca cagccctcgc ggtctcctca
     1501 aaatccctgt acttgcccgt cccaatccag agccttgacc tgaattctat tcccctaatg
     1561 ataagcgtat cttccatacc tcctcctgct cttcctcctc gtttatgcac ttattgcggg
     1621 taccctctgc gctccccctc cccccccgac aaaatacacc acttcaatac tgtctccctc
     1681 ctgtaattta tattccgtgt actccgccct cttgagcacc ttcaggttta tttccaccgc
     1741 aaccctctca ggcactatgt ccatttcccc caaaagttcc ctgaccgtgt tcttttcggt
     1801 gcaatacgct tcaccgttca ccgtaagtct catctcattc tttcctaaaa acaaaaatcc
     1861 tctttcccta aatggggaat agaggatttt ctcccttctc cttattcccg ttccctacgc
     1921 cagtattacc tggatcaggt tcttggggtc ttcctcgcct tcggaaaatc tcaggccttc
     1981 cggcctcccc cagggacact caatatacta ctattatatc aaaaatcttt ctcctccgca
     2041 tatgctcctt attctccttc ccggatccgg tttccattcg ccatgtcttt gtcgggaacg
     2101 ttctctctcc ccttgcctca tccttgacct tatccccgga ctatctttac aatgatttca
     2161 tggctgagat taaaatcctt cccgtcgaag taagaaacaa gattgctgcc ggcgaaatga
     2221 tcgagcgccc cgcatcggtg gtcaaagaac tcatggagaa ttccatagac gcgctgtcca
     2281 ctcatatcag gatagaggtc ctccgtggcg ggaaagggct cattaaggtc tctgacaacg
     2341 gtcgtggaat ggacagggat gacgccctct tgtgccttga acgccatgca acaagcaagc
     2401 tcagctcggc caatgacctc ttctctatca gcacgatggg cttcaggggc gaggcacttc
     2461 cttccatcgc atccgtatcc aggatgagtc tcgtcacagg attgcaggat gccccttccg
     2521 gcgtattcgt tgaggcgcac ggaggagagg tgaaagacat acgggatttt cccgctcaag
     2581 gcacatcggt agagatacgg gacctctttt tcaatacccc tgcacggaaa aagttcctca
     2641 agtctgaccg cacagagctt tttcatatca ttgattgggt caccagggag gcccttgctc
     2701 attgggatat cggcttcaca ctctttacgg aaaaacagga gaccatgagt attcccaggg
     2761 catcagaccc cagagagaga atactccaga tatacggcct ggaattcgta gaagaattgc
     2821 tggagctgca tgcaaaggac cccggtctcg cgataaccgc ctttgtcact aaaggggagc
     2881 atttcaggag cagcaagagc catcagctca tctttatcaa caagcgcccg gtgaaggacc
     2941 agacaatctc ccatgcagtg taccaggcat atgaaggcct tttgccccat gataggcatc
     3001 cggttttttt tcttttcatg gatattgacc caaggagaat agatgtgaat gtgcacccta
     3061 caaagcgcga ggtgaggttt gagaacaaag acctcatcca ccgttttgtg agcagcactg
     3121 tcagggaagg aataaaagaa gaacgcagcc actacactgc gccctttatc gagccatcaa
     3181 tgcacgcctt ccccggccct ggggtgaaag tgccctctcc tgggtataga gaacccgtta
     3241 tgcgcgagcg cggcgtctct gagaactgcg accttcttta ccagccttct ctcccctttc
     3301 tctatctcgg cgataccttt atcgctatgg cgggaaaggg agggttgacc ctcatagacc
     3361 accacgcagc gcacgagagg attctctatg aaaatttcct gaaaggaatg aacctgaatt
     3421 cttacccgtt gctcttcccg cgacagataa agctctccca taaggaatac gcccttcttc
     3481 ttgagaataa agacaccctc catgaaatgg gcctggagat ggacgatttt ggccatgata
     3541 ccctggtcgt gcggagcctc cccgaagccc tggaagaggc tgacctgagg ggaatcctct
     3601 ctgatgttgc cgcagccctt ggggagggga tacgtccctt cagccggatg aaagaagtcc
     3661 tcgctgcaag gattgcgtgc catagttcgg tgcgcggcaa ggacatcctc caccaggaag
     3721 agttccacca actcctgcag cgcctcgaag ataccgagca tcctgaccag tgcccccacg
     3781 gcaggccaac gaggatcttc ctttccctgg acgatctgaa gaagatgttc aagagaaagt
     3841 agaggtcttc agaaaaggac atgaagaagg tcattatcct ccttggtccc acaggtgtgg
     3901 gtaagacagg ggcatccatt ctccttgcac aatcgctgaa tacagagatc atcagtgcag
     3961 actccatgca gatctaccgc cacatggaca tcggtacggc aaaaccctca aagcaagaga
     4021 tgtcgctggt gaaacaccat atgatcgata tcgtggaacc atcggaaacc ttcagcgcag
     4081 gaaaatatat cgagcatgtt acgcccatcg tagaagggct ccattatcag gggaaaatac
     4141 ccgttgtcgt gggaggcacg ggcctgtata tcaaggcaat gacacggggc ctgttctccg
     4201 gaccctctgc agactggcat ctcagggaag aactcctcga tagggaagaa gaaaagaaag
     4261 gctcccttta tgcctctctc atgaaacttg acccttctgc agctgccaga ataatggaaa
     4321 acgatacgag gaggatcgtg agggcattgg aggtctgtct gaaggcggga aggggaatca
     4381 ctcaattgca ggagagcctc accacccctt tgccgtatga tttcatacgc atcggcatta
     4441 cccgggagag gaaggaactc tacggactca tcgagaagcg ggtcgattcc atgctcactg
     4501 cgggtcttgt ggatgaggtg aggagcctgc tcctgaggaa tccagagaag actgccctgc
     4561 aggccatagg ctacaaggag atcgctgccc atctcagggg ggaataccca ctagatgaag
     4621 gggtccgtct cattaaaaag cggtcgaaga attatgcgaa gcgacaaatc acctggttca
     4681 agagggaaga gggaatgcga tgggtggatg tcacaggaca tttcaccggc aaagagatct
     4741 atgaggctat tgcaatattc attgaaaatg aattacctta aagagacaac gatatccggc
     4801 ttcaaagagt ttcaaggggt gccggtgagg aggtgcgatg tccaattctc tgcaggacaa
     4861 ttacctgaat cagctgagga aagacaagac accggtggtg gtgtatctcg ccaacggcgt
     4921 caggttaaaa ggcatgatca aggcctttga taattttgtg atcgttctca aggagtcgac
     4981 ccagcaatta atctataaac acgcggtctc cacaattgtc cctgagaaag atgtcgacct
     5041 gcggttcgaa gaataaaaaa aatccctttc ccacggtaga tctcattata gagtgcgaag
     5101 gggggatcat cctgatcaag agggaaaatc ctccccttgg gtgggccctt cccgggggat
     5161 tcgttgatta cggtgaaagt cttgagtctg cagccctacg ggaggcaaag gaagaaacag
     5221 gcttggaggt ggcgctcgtc aggcagtttc acacatattc cgaccctcac agggacccga
     5281 ggcaccacac catcaccacc gtctatatcg caaaggcgaa gggaagaccg gtggcaggtg
     5341 atgatgcggc agaggcgatg ctgttcagcc aggcaacact cccggaatca atagccttcg
     5401 atcacaagtc gatactggat gactacttta cgaaaaggta ttgaaatgtt atacactatc
     5461 ctcgtcaagt tctgtactat tcatcgctct gatatagata attaaatctt tctttacgga
     5521 ggtataaaat gctcagggca atgcgtaaac atgcaaagta tttctatgtc cttttcttca
     5581 ttgtgatcct ctcgttcatc ttctggggag tgggaaccat ggataaatcc accggcgtcc
     5641 ctgtcgccga agtgggaaag gaacggataa ccgttgaaga atactggacc tcgtacgata
     5701 gggccaggga attttaccgg accctctcgc aagggaactt caccgaagag gcggagaaaa
     5761 agctcaacct gaagcaacgg gtgctggatt ccctcgtcga tgacctggtg ctcatgacag
     5821 aggcgaaaaa ggccggaatt cgggtgtctg atggggaact ggctgacgcc atcgtcaatg
     5881 agcctgcctt cgtgcgcgat ggcacattca ataaggagat ctacgtcaga actctccagc
     5941 tcaacaggat gaccccggaa atgtttgaat ccatgaagcg gcgggaatta accctcgcca
     6001 ggatgcgcaa gctcatcggt gaatctgtcg atgtcctggt gagcgatgtt ccccaggctt
     6061 caggaaatga acaagccctt gcgtcccttc gccagactgc actccccgag atgcgggaca
     6121 gggcggtaaa atcctacatt gagggactca aacagcagat gaagataaag gtgaatcagc
     6181 agctgataag ctgatgcagg ggctgcccgg aatcgtgcac ctcaccttcc cggggtgacc
     6241 ggcattagag agggatatgt tcggaaaccg tttttaccgc aacgctgccg ttacggcatg
     6301 ggccacggca gtgatctcct ggtcaccaat ggtcctcgca tccagtatga gggaattccc
     6361 ctgaatcctt gcgataatgg ccggctctcc ctttctgagc ctttcttcca gctcattcac
     6421 cgaaatattc tcaggtctga tggccaccac gtaagtcgga aactccactt cggggaggga
     6481 gcctcctcct gcctgggatg agtcttccag aacctgcagt actgcatcct ttatctcgcg
     6541 tttaatgaga gaagcgatct ttttcgcgcg cgtctttatg gttccgggat cttccagcag
     6601 cattctcagg gttggtatgt gctccttcgc ctgcgcccca tccacatatt ccatgagggt
     6661 cgcctccaac gcagcaaggg tgagtttatc tatccgcacg gccctggcga ggggatgctt
     6721 ctgaatcttt tcgatgagct cccgtttgcc cacaataatc cctgcctggg gaccaccaag
     6781 gagtttatcc ccgctgaagg tgatgaggtc agccccgctc ctcaccatct cctgcactgc
     6841 cggttcccca tgtattccgt actgatgaag cggtatgaga tacccgcttc ccagatcata
     6901 catcacggga actcctcgcc cttggccaag ggagacgagc ttctctacgg gaacctcctc
     6961 ggtaaatccg acaatctggt agttggacct gtggactttg agaatgaggg cagtggcatc
     7021 actcatggca tgctcgtagt catggaggtg tgttttattg gttgtcccca cctctctcag
     7081 gagcgccccg ctatgggaca tcacatcagg aatcctgaac gagcctccta tctccaccag
     7141 ctctcccctg gagacaatga cctcccttcc cttggcgaga gcggtcaaac agaggaagac
     7201 cgccgcggca ttattgttca ctgcaaaacc gtcctctgca ccggtgattt ccctcaagag
     7261 ccttttgatg tgggtatgtc gcttcccccg cgagcctgag gcgaggtcat actcgagatt
     7321 tgaataccct gtggcgatcg tgctcacatt ctccatgatc cgctcagaaa gaatggaccg
     7381 tccgaggttc gtatgaacga cgattccggt tgcgttgatc aggcgtctga ggcttggggt
     7441 tgccaatcgt ttcacattta aactgatatc aggtgccatg ctcgccagcg agacttcctt
     7501 tgtagtcccc tcaaggatct cttttctcct aagggaaatc gtctcccgta tggcgtcaag
     7561 gacataccgt ctcggatact tctccagcca ttctctcccg tacccgctct tgagtgcttc
     7621 atccacagag gggaggcttg aaagaagatc ccttgtatcc atacggatgt tacccttgcc
     7681 cgttcaggat atacttcacg ctcaccttca ggtctttacg gagggaatgg tatacggaac
     7741 agtacttctc ctgggaaagg gcaacagccc gctccacctg tttctgagta atccgttccc
     7801 ccgagatatg cagtaccatt tcgatggcct tatagtactg gggtggcgta gggtttctct
     7861 cccctgtaat gtctatttta aagctcgtga gattcgccct cattttttgc aggaatgaga
     7921 ccgcgtcgat ccccatgcat gccgcgaggc tcaacaaaag gctctctgtg ggtgcgcatc
     7981 cccactgaat ctctgcgtcg aactctatct cgtatcccct ctgtgtccgt cctacaaaga
     8041 tcagatcctt gtcccaggtg agggttccct tcgtgacagg agccaccttt tctttatatc
     8101 ctgttaatga ctccttcagt tccccggggt caccgcgttc catttccata tttttactcc
     8161 tcatgcagaa aggtggctct attttatatg gagaagacgt atttggtcaa ttaagtcttg
     8221 atagagttat ccctttcgca tacaatacca catgcgctat gtaattctcg gcacagcagg
     8281 ccacatcgac cacggcaaaa gctccctggt gaaggccctt accggcatag acccggacag
     8341 actcaaagaa gaaaaagagc gggggatcac catagacctg ggcttcgccg atctctcgta
     8401 tccggacggc ctcactgtag gtattgtgga tgtccccggg catgagcgcc tcgtgcgcaa
     8461 catgcttgca ggggcagggg gcatcgacct cgtgctcctt gtcatcgctg cggatgaagg
     8521 gatcatgccc cagagccgtg aacatctcgc catatgcaac cttctcagga taaagaccgg
     8581 tcttatcgcg ctcacaaaga ccgacctggt ggaaaaagaa tggctggaac tcgtaaagga
     8641 tgaggtaagg agctttgtga ggggttcctt tttggaaaac gcagagatca tccctgtctc
     8701 ttcaaaaaca ggggataacc tggaactgtt aaaagagaga ataagggacg ttgcccttac
     8761 ggtcgagcaa aaaccggtaa gagagatttt caggctcccc atagacaggg ttttcaccct
     8821 gaagggcttt gggaccgtgg taacgggaac ggtcatttcc ggcaccctgt ctttggatga
     8881 catggtggag atagagccct caaagatcca taccaaggta aggggacttc acagccacgg
     8941 gaaagcaatc ggaaccgcct acgcaggcca gagggttgca atcaatcttc agggggtcaa
     9001 caaagaggac ctgaagcggg gggatgtggt ggtcactcca agggtggtct ctgcatcgag
     9061 gattatggat gcaaaaatag aacttctcca tgacgtcccc cccctaaaga gcaaaagcct
     9121 tgtgcacctc catatcacca cgtcggaatc catcgcgagg gtaattcttt acaacaggga
     9181 ggagctgcgg ccgggggagt cctgctattg ccagctgagg ctcgaagaac ccgcggtggc
     9241 actttcgggg gacagataca tcgtgaggaa attctccccc gtcatcacca tcggcggagg
     9301 aacaatcctt gacccttccc ctctccggag aagacgcaag gaggcactgg aggatctgga
     9361 tgtccttgaa cagggagccc ttccggagag gatcgcagtg aaagtgaaga aatcgggaat
     9421 aaagggaatg aaggcattgt ccctctccgg gtggataaag gcagagaccc actccatcga
     9481 cgaatccctc tctctgttga aaaagcaggg aggcctgaag cagctcgggg agaccctcct
     9541 tcatcgtgac atctataccg cactggcaaa agggattacc gaaactctct cctccttcca
     9601 taaaaagaat cccctcagcc ccgggatgct caaggaggag ttgagagcac agctcaaagt
     9661 gggccctgag ctatggaaca cccttttaag ttcactggaa gccgtactcg tggataagga
     9721 tgtggttcgc atgaaagatg cgattattgc ctcatccgtg gcctcccagt catcggagaa
     9781 gatactccag cttctggaaa aagccaggtt ccagccgccc tccagggaag aactctcgga
     9841 gtctctcaag atggatccaa aggcattagg ggatctcctt aagctgatga gcaaagaggg
     9901 ttcggtggtg aggatcaacg attccctgta tgtcgcgggt accgcgttta aggagatgat
     9961 ttctctcctc agaggatttt ttgcaaaaaa acccgccatg accgtagctg aattcaggga
    10021 catcctggat actacacgga agtacgcgct tccgttcctg gaatatctcg attcaaacaa
    10081 tatcactctc cgggtgggag atgtgaggaa gtttcttctg aaatgacgaa ccgatgaaac
    10141 tcaataatgc ggtatccgcc tttttcgatg cggggaggga ggaatggtgg gcagtcgcag
    10201 aatcgaactg cggacacgag gcttttcagg cctctgctct accgactgag ctaactgccc
    10261 gtgttataaa ctatagtcgg tgaggctttt tattgtcaag gaagactcaa atgaatacac
    10321 agactaaggg ggggatgcca ttgaagctgc tgctgcgcac ctaccttccg gcagccattt
    10381 ccgggctcct gctgttcgcc tcttttccca ccatcgatct tttccccctc gcatggatag
    10441 cccttgtccc ccttatgctc tccctctggg acaagggccc gcgggctgcc ttcttttcgg
    10501 ggctgctcac cggcaccctg tatttcttcg gaacccttta ctggatttac cattccatta
    10561 cctattatgg ctctgtcccc ttcatcatca gcgttgttct ggtcctcctc cttagtctct
    10621 atctcggcct ctatgtcggc ctcttcgccc ttgtgttttc ccttaagatc aggcatacga
    10681 gacttcctgc gctcatactc gcgcccgtgt tctgggtgac ccttgaattc atccggtcct
    10741 atgccctcac cgggttcccc tgggcaagta taggctattc ccaataccgg tttctctacg
    10801 ggatccagta tgcggatatc accggtgtgt acggtgtctc tttccttgtc ctggcggtga
    10861 acagcgcaat tgccgatttc ttcattctca aacgccgtcg tgccgagatg cccctctact
    10921 ccgtgtccta caccttcatc agttatgctg tctttttcct cctccttctt actctcttcc
    10981 cctacagcta ctggagaatt catcagcagc gtcccgggaa tcctgtcagg gtgagcatta
    11041 tccaggggaa catcgaacag gataagaaat ggcagccggg gtatcaggat gaggtgatgc
    11101 gcatctacaa agaattaacc acaaaggcta ctgcgtccac gccctctttg gtaatctggc
    11161 ccgagacatc cgtccccttt tatttcggat atgatactgc acgctcccgc gaccttgtcg
    11221 agttccagcg aaggataaac tcatatcttc tcttcggaag cgtcatggta aaaagccctc
    11281 ctgcgaaacg tcagcccgtt caactgacta acagcgcggt cctgctggac ccggagggaa
    11341 atatctccta tgtttatgat aaaatacacc ttgtcccctt tggagagtat gttcctttgc
    11401 gcagtatctt tttcttcttg gataaacttg tggcgggcat cggggactat gagcccggca
    11461 acagggtgat aaaggcaaac acggggatag ggagcttcgg aacctttatc tgctatgaaa
    11521 tcatcttccc cggactggtg agaaagtgtt tctcaaaaga cggggatttt atggtgacca
    11581 tcaccaatga tgcgtggttc gggaggacat ccggtccctt ccagcatttc agcatggcgg
    11641 tgttcagggc aatagaaaac agaaagcctg tgctcagggc agccaacacc ggtatttcag
    11701 ggtacatcga cagtaatgga aaggtgctca tgagcacttc ccttttcgag cgacgggaag
    11761 agacagtgga aatgaccaca gatagcagca ggagttttta ctcaaagtat ggggacatat
    11821 tctcctatat ctgcatcgtg gtttcgcttt tgttattaat ttagaggagg tacggttatg
    11881 ttgcgtattg acgagataag gcaggacctg gtaagtctca gggtaaagat agaaaccctc
    11941 cgagggtatc tttgatgtac cacatcttaa ggaagatata gttcgtcttg aaggtgaact
    12001 ctctctagag tcctcgtgga catccccgga aacacttcac gtagtgcaga agaaaaaggc
    12061 agctttggaa ggagtcgtat ttccttttga tgctgctgct gaaagactct cttatcttga
    12121 agattccttg gacatcctgg aggaggaagg gggcgacgcc tttctgaaag agatccagga
    12181 ggggatagca gagctcaggg aatccattga gcgcatagaa ttgagacact tcctttcagg
    12241 ggagctggac ctcaacaacg ctattgttac aatccatccc ggggccggag gaacagaaag
    12301 ccaggactgg gcccaaatcc tcatgcggat gtatttcaga tgggctgaaa cgaacggctt
    12361 cactacagat gtaattgacc tgcagcccgg agaagaagcg gggattaaaa gtgtcacctt
    12421 cacggtcacc ggccagtatg cctatggata tctgaaggcc gaagccgggg ttcacagact
    12481 tgtgaggata tctccctttg atgcaaataa aagaaggcat acctcctttt ctgctgttct
    12541 tgtctttcct gagattgagg agaacattaa ggtcgacatt aacgatgatg atcttaagat
    12601 tgacaccttc agggcatcag gggcaggcgg ccagcacgtg aataaaacgt cttctgcggt
    12661 gagaataacc cacattccca caggaattgt cgttacatgc cagaacgagc ggtcacagca
    12721 caagaacaag gggacggcgc tcaaaatact aagggcaagg ctttatgatc tccagcagga
    12781 agcacaggaa agaaaagtgg aaggtattgt gggagacaag aaaggaatat cgtggggaaa
    12841 ccagattagg tcctatgttc tccagcctta caggcttgta aaggaccatc gcacaggact
    12901 tgaggttggg aatgtggata gtgttcttaa tgggaacatc aatagtttca tagatgcgta
    12961 tcttagagag aaaggtaagg tagtacagta agctctacta ctattcttat ataagtattt
    13021 tagaaacagt agaagaaggt gtgaataagt gaagagggta ttgctcatcc tgatgtaaaa
    13081 gccttttagg gattatgaga tgtgaacata tccggtatag tccggagact catggggaaa
    13141 gtccggggcc ttggacgtat cccacacatt atgcacaggg aagcagttgg cagcttgcca
    13201 tgggtgtgaa tatgaggtat aatatttcgc ctgcaaaaag aggttactaa caactgttag
    13261 taacatgtga ataacttggg ggagtcttga agcccttaat tttttcgaac agacacatat
    13321 gccattagaa gccatttgga ataagagcct gacaattata gagaaaaagg tcggagagaa
    13381 cgcctttgaa ttatggttta aacccataaa actctctcag ctaaaggacc agagcgcggt
    13441 catcgagatt cccaacaggt ttttcaagga ttgggtggaa gactactacc cgacccttat
    13501 caacgatact atggaggaga tcgtcggtca tccaataacc gtaaaattca agatcgcgga
    13561 aaaagaggat tcagaggtaa agaggataga tgcccgtctc gagggacgaa aacagcgtct
    13621 cgcgagcaaa gggatctacc tgaatccgaa atacaccttc agtaatttta ttacaggtca
    13681 gagtaatcag tttgcccatg ccgcggcaat ggcagtggca gagtctctgg ggaaggcata
    13741 taatccgctt tttatctatg ggggagtagg gctcggtaag acccacctca tcaccgctat
    13801 aggaaacaag gtagcagaca aaagacagga tttcaatatt ctctatgttt cctcagaaca
    13861 gtttaccaat gaggtggtct ccgcggtccg gcacggaaag accgaggagc ttaagggcaa
    13921 gtacaggaac cttgaccttc tgctggttga tgatgtccag ttcatagaga acaagaccgc
    13981 tacacaggag gaacttttcc atacactcaa caccctttac gagaaacaga agcaaatcgt
    14041 gctctccagc gacagaccgc ccaaagagat caagaatatc acggatagat tgagatcacg
    14101 cttcagcatg ggtctcatcg cagacattca gccacccgag atcgaactca gaatggccat
    14161 aatccagaag aaggctgcca cggaaaggat ccagctcagc gatgaggtag tgaatttcct
    14221 tgcctcccgg gtgaaatcca atatcaggga acttgagggt tctctcataa ggctgggtgc
    14281 acagtcgtcc ctcaccagtg agcccattga tgtgagcatg gcgaagaata tactcaaaga
    14341 tatcatttct gaggttgaca ggcccatcac catcgagcag atacaaaaga gtgccgcgga
    14401 attttacggc atcaaatacc aggagatgaa atcaaagaag aggacaaagg agatcgccat
    14461 cccacgtcag gtggcgatgt acatcagcaa gcagctcacc ggctcctctc tcagtgatat
    14521 cggaaagaac tttggcggca aggaccatgc cacggtcatc tacgcgtgca agcagataga
    14581 gggaaaaagg gaaaaggacg aggagttcaa cagaaaggtc gaaagtctca taaaacggat
    14641 acaaggatag caaggaggtt cccatgaagc tgactattgc aaaggacgag attcaggaga
    14701 aattgtcaaa tatccagaac attgtggaaa aacgaaacac catgcctatt ctcagccatt
    14761 ttctcctgga cgcgggaaag aaagagaccc atatcgtggc cacagacctc cagatcgcga
    14821 tcaaagagcc cctgaaggcg aacgtacaga gtgagggaaa actctgcatc ccggcgagaa
    14881 aactgttcga gatcgtacgg gaggcggagg gagatattac catagaatcc gaggatgagc
    14941 agtggataaa ggtttccttt ggaaagagca ggttcaggct cgcctgtctt gcttccgcgg
    15001 aattccctgc atggcccggg attgaaaccg gtgaagaggt gaagctcggg atagataccc
    15061 tttcggagat gatcgaaaag accatttatg ctgcggggga gacagatacc cgttacaccc
    15121 tcaacgggct gctctttcat atacggccgg cagacacgac ccttaccctt gtgggcactg
    15181 atggtcatcg gctcgcgctg ctgagtaaag ggctggaatc caaagcggag gaagaaacga
    15241 aggtgatcgt gccgagaaag gcagcggctg agctccggag atttttcggc agttccgaga
    15301 gcgtatctgc ggtgatgggg aaaaaccatc tcctctttcg catcggggat gtccagttcc
    15361 tggtaaggct tatcgaaggc acctatccca attatgagca ggtgatcccc ccctccaatg
    15421 aaaagaagat gaccatccac agggaaggtc ttctgaggac cctcagaagg gtatctataa
    15481 tgtcaaagga acggagcaat gcggtgaagg tggatatcac ccagggctcc cttgccatct
    15541 cgtcttcaaa tcctgacctg ggagaagcac gggatgagat cagcgttgag tatgcgggag
    15601 aggacctttc ggtgggattt aatgcccgct acctcatgga cgctctgggt gccatgactg
    15661 cggaaaaggt ggtattcgcc cttcaggatt ccctgagccc taccctcctg aaggaggaag
    15721 gaaatgacga atacaagtgt gtagtcatgc ccatgagaat ataagaccgt ctccgtacac
    15781 cggcgaagta cgcacgcaca gagctcacgc gtgagcttcg gcatgtgcgc gttcagtacg
    15841 aaaaacagag aaaggaatta atggaagaga aagataaaga aagaaacagg gaaaaaatcg
    15901 aggccgaggc cgaggcttac ggggctgagg acatcaagat actgaaaggc cttgacgcag
    15961 tgaggaagag gccggccatg tacatcggtt ccacaggccc tgacggcctg catcacctgg
    16021 tatacgaggt tgtggacaac agcgtggatg aggcccttgc cggattctgc aatgccatcg
    16081 atgtgatgct tcaccatgat ggaagctgca ccgtgatcga tgacggaagg ggcattccca
    16141 ccgcgcccca tccgggggac cctgaggggc ggtctgcggc agaggtggtg ctcaccgagc
    16201 tccacgcggg agggaagttc gaatccaagg cgtataagat ttccggtggg ctccacgggg
    16261 tgggggtctc ggtggtcaat gccctttccg agtggcttga agtggagata aagcataacg
    16321 ggcaggtctt ccagcagagg tttgagaggg gcaagcctgc aggaccattg agtgttgtgg
    16381 gaaagacgaa gggccggggc acaaaggtca ccttcaagcc cgatcctgag gtcttcgaga
    16441 tgacggactt cagctatgac gtgctttccc agaggttgag ggagctcgca tttctcaaca
    16501 gggggcttaa gataaccatc ctggatgaaa gggtggacaa gaaacaggag tttctctata
    16561 agggaggcat cgtctctttt gtggaacacc tcaataagaa caagacagcc ctccatccga
    16621 aaccgatata catatcaggg gaaaaagagg agatctttgc cgaggttgcc ctccagtata
    16681 atgacggata cgcggaaaac atattcacct tcgccaacaa catcaacacc aaggaagggg
    16741 gaacgcacct catcgggttc aagtccgccc ttacaagaac ggcgaattcc tatggctctg
    16801 catcagggat tttgaaaaac ggcaaagaga cgctttcggg tgacgatata agggagggct
    16861 tgaccgcggt ggtgagcgtg aagcttccca atccccagtt cgagggccag acaaaaatga
    16921 agctgggcaa cagcgaggtg aaggggattg tggaatccat tgtgaacgag accctggcta
    16981 cctattttgg ggaaaaccct tccattgcga ggaaggtaat tgaaaaagcg atccaggcat
    17041 caagggcaag ggaggctgcg cgaaaggcaa gggagctcac gcggagaaag ggtgcccttg
    17101 aggataccgg actcccgggg aaactcgcgg actgttcgga aaaggaccct gccctgagcg
    17161 agctcttcat cgtggagggc gaatccgcag gcggctccgc caaacagggg agagaccgca
    17221 ggacccaggc gatcctgccc ttgaggggca agatcctgaa tgtggaaaag gcacgctttg
    17281 acaagatgct cggccatgaa gagatcaaga tactcatcac cgccctggga acaggcatcg
    17341 gcgcggatga ttttgacatc gcgaagatca ggtaccacaa ggtcatcctg atgaccgacg
    17401 ctgatgtgga cggcgcccat atcagaaccc ttctcctcac ctttttttac cgccagatgc
    17461 cgcaggtcat agaaaacgga tacctctata tcgcacagcc ccctctgttt aaggtgaagc
    17521 gggggaagac cgagaggtac atccagagcg aggcagaact ccagaatatg cttttcgagc
    17581 tcgcgtcaga tgacctctcc cttagcatca agggacaaga ggtaaagggc aaggcgctca
    17641 tccctttcct gaagcgtctg ctgaattttg agagattgct ggaatggttt gagaggaggc
    17701 gcagggaccc cctggtgctc aggatctttg tggcagaggg gattaccaag gcgctcatga
    17761 aagatcggga gaagatatca acgcttctcg aaaaggtgac gaagcagctc cctgacgcga
    17821 acatcggtga cctttacgag gatgaggagc atgaaggata cggggtggag ataagaaggc
    17881 agagttacaa gctcgtcctg gatacgaatc tccttttatc cccggagttc agggagctgg
    17941 agaccctcta cgccttcatg aaagatcttg gcaaggcccc ctttaagatc tccacaaagg
    18001 acgggcagaa agaggtggcg tccaccaccg ggctccttgc ccttatcgtc gaaacagcga
    18061 ggaagggcct ctccatccag aggtataaag gcctgggcga aatgaacccc cagcagctct
    18121 gggataccac catggattct gacaagagga cattgctcca ggtgagcatc gaggactctg
    18181 tgcaggctga tcagatattc accatcctca tgggtgacca ggttgaacca aggaaggagt
    18241 tcatcgtcaa acacgccttg gaggcgagga atatcgatat atagattgca ggattcttct
    18301 gggttttcac aaaagagaaa atataaagct tatcccggca gttcctcaca ggagttctca
    18361 ctatgcagca tcctgattat cctgcaaaat acgagcggga atctttacag gtgccagtac
    18421 cttcctaagt tgcaaaatac tacggaaaga gactaatatc taaggcaata agggaggggg
    18481 atggggagaa ctcctgaaga cgaagcacgc caagttattg acggtctgct tgaaaaggcg
    18541 ggctggcatg tctgcgatta taaagaagcg aacatccata gctcaaccgg agtcgtgata
    18601 agaaactttc ctctgaagac aaaacacggt atcgccgact atctcttcta tattgaaggc
    18661 aaggcagcgg gcgtaataga ggctaaaaaa gaaggcacaa ctctcacagg cgttgaaatc
    18721 cagtcagata agtataagaa tggtctcccc gagattctcc cgtcctggca tcggcctttg
    18781 cccttctgct atgaatccac cggggttgag acaaggttca ccagttatct ggacccggat
    18841 gcccgatcaa ggcaggtttt ttcccttcat cgccctgaaa cgctccatga atggctgaca
    18901 tattcaggag aggcgacact cgacaaggca gccgacccgc tggggattta ttggccgcct
    18961 tccacccttc gctcacgcct ccgcaagatg ccgcagctca tcgaaggagg gctgtggcct
    19021 gcccagataa aggcactcat gaaccttgag aaatcaatga gcgaaacccg gccccgtgct
    19081 gttgtccaga tggcgaccgg aagcggcaag acattcactg ccgtcacctt catctaccgc
    19141 ctgcttcgct tcgcaggggc aaagcgtgtc ttgttccttg tggacagggc caatctcgga
    19201 cgccagacgc tcaaggagtt ccagcagtat cagtctcatt acaatccgta taaattcacc
    19261 gaagaatata tcgtccagca gctcacttcg aacaaaatag acaagaccgc cagggtctgc
    19321 atctcgacga tccagcggct ctattctata ctcaagggcg aagagcttcc cgaagaggag
    19381 gacgagcatt ccaccgcagg gctggagtat gtcatcaaag agccgccgcc catcgaatac
    19441 aatcccaatg tgcctatcga gacctttgac gtgatcgtca ccgacgaatg ccatcgctcc
    19501 atctataacc tctggcggca ggtgctcgaa tacttcgacg cctttctcat cggcctgacc
    19561 gcaactccga acaagcagac cttcggcttc ttcaaccaga accttgtgat ggaatatccc
    19621 catgagcagg cggttgccga tggcgtgaac gtgaattacg acgtctacaa gatccgcacc
    19681 aaaattacag aaggcggctc gaaggtcgat gccggttaca ttgtggacaa gcgcgaccgt
    19741 gagacacgta cagttcgctg ggaaaagctg gatgaggatt tcgcgtacac cgctgagcaa
    19801 ctcgaccggg atgtggtggc aatcgatcag atacgtacag ttgtccgcgc cttcaagaat
    19861 aagctcttta ccgagatatt ccccggcagg accgaggtgc ccaagacgct catctatgca
    19921 aaggacgatt cccatgcaga ggacattgtc aagatcgtgc gcgaggagtt caacaagggc
    19981 aatgagttct gccagaagat aacctataag acaacaggaa agaagacgga cgaactgatc
    20041 gcagaattca gaaacagcta cttcccccgc atcgctgtca ccgtggacat gattgcaacc
    20101 ggtacagaca taaaacccct cgaaatcgtc tttttcctca ggcaggtcca gtcccgcgcc
    20161 tactttgaac agatgaaggg gcggggcgtg cgcgtcatca acgagacaga cctgcaatca
    20221 gtaacgccgg atgcaaagtc caaggaccat tttgtggtag tggatgcggt cggcgtctgc
    20281 gaggctgcca aaacagattc ccgacccatg gaccgccagc ggaccgttcc tctcgaaaag
    20341 ctactccagg ctgttgccct cggcagcact gatactgata tcatttcatc ccttgcggcg
    20401 aggtttgcgc ggcttgagcg caggatcaca aaagaggaca atgcagaaat aaggaagttt
    20461 gcagatggca agggacttaa ggaactgaca gggagtcttg tgcagtccct cgatccggac
    20521 agacatattg agcttgcccg cgccgagcaa agcgaggcag gcgggacagg cgagccgaca
    20581 gaggaacaga tcaagaaagc ggctgaaaag ctcatccaaa gtggggtgac gcccctgcat
    20641 aaccccaggg ttcgggaaaa gatactggag atttataaaa agacagagca gacaatcgat
    20701 actgtcagca ctgatgaagt cacaactgtc ggcttcagcg cggaggcgct ggagaaggcg
    20761 aaggggctgg tgcagtcctt cgagcagttc atcaaggagc acaaagacga aatcacggca
    20821 ctccagatcc tttacagcaa gccgtataag acacggttga aatatgagca ggtgaaggag
    20881 cttgcaaacc tcatcgaaaa gccgccgtac ttctggaagg ttgacaacct ctggaatgcc
    20941 tatgccgcgc tcgaaaagtc aaaggtccgt ggcgccagcg ccccgcatat cctgacagac
    21001 cttgtctcgc tcgtccgttt tgctatgcat caggagaatg aacttgtgcc ctttcccgag
    21061 cgggtcaatt ctaatttcag tgcgtggatt gcccagcagg agaatgctgg caagaaaatt
    21121 accgacgaac agcgtcgctg gcttgaaatg atccgtgatc atattgcggg tagcctctcc
    21181 attgagacag acgactttga gtatggcaag ttcattcaac aaggtgggat tggaaaggcg
    21241 tatcaggtgt ttggggatga gttaagtaga atgattgaag aattgaatgg agttctggcg
    21301 gcgtgagaag agctgagatt gacgaaaaag aacttttggg ataaaatagg tactgaaaca
    21361 aatacggaaa ggagtactgc aatgaatact ttagctgcac aggaaataaa aaggaagggg
    21421 atttcggcag tagatgaagt cctgaaagaa ggcccggttc acattattaa gaataatcag
    21481 cctcagtatg tagtccttac ggaagagcgc tatcgcgatt tgcttgaggc agaggatgag
    21541 gcatacaatg cgcgaatccg tgcatccctt gaagacctga aggcagggag agtaaaacgc
    21601 ggaactgcta aagatttaat taaggaatta ggactggaag attgaggcat gtatgctctt
    21661 gtatggactg cccatttcac gcgcgccgcg aagaagtttg ggaaaagcca tcctgagctg
    21721 aagaagaaag tcgccggcat tctccgcgat ctcgaaaaag acccttttca gccacatctg
    21781 aaatatcacc acctcggggg caaatataag gggatgcagg caataagtat taccgacagc
    21841 taccggatca ctatgaccgt catgattact gagaaggaaa ttattctgat cgacataggt
    21901 acgcatgatg aagcgtatcg gtgaaaccgc gcagcccggt gaaggcgtgt gctgatagta
    21961 tcctcagttt ttcaaaagat ttcatgacga agagaaatca gccgccgctt caagaaaagg
    22021 catcactgga tcatgagaaa cagaataata cgccgctgcc ggatgggtgg gtgtggaaga
    22081 cgttgggaga ggttctatca gacatcgtgg gaggtggtac gccctctaaa actaactcaa
    22141 aatactggaa cggtactatc ccttggttga ctgtcaaaga catgcgaacg cgccgcccag
    22201 ccgactcgat tgatcacatc tcaaaagatg caattaggga gagcgcaacg aatcttatac
    22261 cgaaggatac tgtgattatc gccacccgcg ttggtctcgg gaaggttata cgtgttcctt
    22321 atgatgcgac aattaatcag gatctcaaag cattaattac cacagcagaa gtagataagg
    22381 gatatctcga acattggatt agttcgattg cgaactattt agaatctatc ggttcaggaa
    22441 caacagtgaa aggcattcgc cttgaaacct taagggagat tccgtttccc cttgcccctc
    22501 tggatcaaca agaacgtatc gtctccgaaa tcgaaaagca tttctcccgc ttggatgagg
    22561 cggttgccag tctcaagcgc gttaagtcga acctcaaacg ctacaaagcc gctgtcctca
    22621 aagctgcggt cgaggggaaa ttgacagagg agtggctcaa ggcacatccc aatgttgagc
    22681 cagcaggtga attgttaaaa cgcatcctcg ccgagcggaa gaaaaaatgg gaagaaaaga
    22741 atccgggcaa gaaatataaa gaacctgccg cacctgatac ttcaaacctg gccgcattgc
    22801 cagcggggtg ggtgtgggca acggttgagc agcttgcagg taagattcag tatggttcct
    22861 cctcaaaaac taatgaagac cgacagggtg ttccagttat aagaatgggc aatatttttg
    22921 aaggtcgact taaattagat gacctcaaat atttaccgcg aaatcattct gaattccctg
    22981 aactctttct caaaaagaat gacctcttgt tcaatac
//
LOCUS       DATKOU010000118         3757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4390035, whole genome shotgun sequence.
ACCESSION   DATKOU010000118 DATKOU010000000
VERSION     DATKOU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390035"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VMU21_05490"
     CDS             <1..1366
                     /locus_tag="VMU21_05490"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HUO77014.1"
                     /translation="GESIPAVQNNPLLQGLKKDYAAAESEYAQLSKIYKPEYPKMVRL
                     REQSNQIKMRIDAETKKALEGLRVDYEAALKKENYLASAMEKYKSEVTGQNEKMVQYQ
                     ILKRESETNRELYNDLLHRLKEIGLSASLSSSNVQILDRAEVPRSPFKPDKKKNLMIA
                     LFVGLLGGIGLAFFAEYLDNTVKTPDDVEKEVALPSLGLVPELGKNNGKNNRPFITYE
                     DKKSPLSEAYRSIGTYIQFSTAGRPPKTILVTSARQGEGKTTTVVNIAVTLVHTYGRG
                     VIIDSDMRRPQIHKVFDVDNSRGLSSFLTGHIEIDQGLIQKTKVENLDVIPAGIIPPN
                     PSELLSSYRFKDLMTGLFPMYSFIIIDSPPVLGLSDSLLLSTLIDGVIMVTRAGNTPK
                     DAAIQARKLLQGVNAKILGVVLNGITEADLKYGSYSYYYSNYYGDGYDSHKEKKKRRA
                     TDHA"
     gene            1311..2612
                     /locus_tag="VMU21_05495"
     CDS             1311..2612
                     /locus_tag="VMU21_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77015.1"
                     /translation="MDTIVTRKKRRGEPRTTLNVSFRVVLTIVSITVTLALVRPALSV
                     YYSQKISELDKERLVRASRITPEDAWYQYLLGFLDYAAHDRPGIERAIGHYLLSLKRN
                     PTDSHAWLAIAKAYRDIDRKDDASYALRKAYYLDKNTPDVIWELALFLLLENKQDEAL
                     PLLRRYITLVPEEQENVYTLCYMMRVKPVSILNDLVPADYSFYNRYLAFLIGDGLLNE
                     STEVWKRIKTYNPERSEYLKYCDFLIASGEVGEGLALWDDFAKRFLDVKDRPEGEILW
                     NGDFELPIENAGFDWKIGKADGVRIHRDQDIKWTGFASLSINFNGNTNPGISLAQQIV
                     PVHPGQKYKLSGHIRTEKITTQNGIVFEASNYLCDSFVKKTEPVTGTTLWKKIELEFT
                     TPQRCKAITIAVKRERSEKFDNKISGDAWIDSLSMTQIKPK"
     gene            2570..>3757
                     /locus_tag="VMU21_05500"
     CDS             2570..>3757
                     /locus_tag="VMU21_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUO77016.1"
                     /translation="MDRFPVNDSDKTQVIFKNALEFIGAVRAGISRERFCTYAVRFSD
                     AFIDMLNDTEEARNFLSSRGISTDYVQSNVKTIFARLHLNKENDHYLTLALPRTATEA
                     QIHKRWKELMRIYHPDRNSEEDAAICAKWINEAYSILKNPGKRMEYDRKTAKTVESHF
                     TRRRSGVIHSNKSGRRLFISPEVRRMVPKLIIASCIAVSCIVLLVMFLRNRLEVYTYQ
                     ASALPDQFMQDAGQAHRTPDIEMGDPKKKEESNVMGFQPAEPPGQDTKTSTGALPEKS
                     QKDKETEGARVKAFNPSGSHTRSAGFATLRTSPAAQEKSMVDTKAGPTEKTVREQIEG
                     SALDTMVQTASPDQNIMKDISPASLQKKSSPEQMSVSGEIANASPPVPTRKEDHPNLE
                     TEVF"
BASE COUNT         1103 a          880 c          916 g          858 t
ORIGIN      
        1 tggagaatct atccctgcag ttcagaataa ccctcttctc cagggcttga aaaaggacta
       61 tgcagccgcc gaatccgaat atgcacagct ttccaagatc tacaaaccgg aatatccgaa
      121 gatggtgaga ttgagagagc agtcgaatca gatcaaaatg aggatagatg ccgagaccaa
      181 aaaggccctg gaagggttac gggtggatta tgaggcagca ttgaaaaagg aaaactatct
      241 cgcgtctgca atggaaaagt ataagagcga agtaacgggc cagaatgaga agatggtgca
      301 gtaccagata ctgaagaggg aatccgagac gaacagggaa ctgtacaatg accttctcca
      361 tcgccttaag gaaatcggcc tttcggcgag tctgtcctcg agcaatgtcc agatcctaga
      421 cagagcggag gttccaagga gccctttcaa gccagacaag aaaaagaacc tgatgatcgc
      481 cctttttgtc ggtctcctcg ggggcattgg gctagctttc ttcgccgagt atctcgataa
      541 tacggtgaag actccggacg atgtggaaaa ggaagtggcc ctgccttccc tcggccttgt
      601 tcctgaactc gggaaaaaca atggaaagaa taacaggcct tttatcactt atgaagataa
      661 gaagtctcct ctgtcagagg cgtaccggtc tatcggcact tatatccagt tttctaccgc
      721 aggcagaccc ccgaagacca tattggtgac atccgcacga cagggcgagg gaaaaacaac
      781 tactgtagtg aacattgcgg ttaccctcgt gcatacctat ggaaggggtg tcattattga
      841 ttctgacatg cgcaggccgc agatccataa ggtatttgac gtagacaact cccggggcct
      901 ttcttccttt ctcacaggac atattgaaat tgatcagggg ctcattcaga aaacaaaggt
      961 ggaaaatctt gatgtcattc ctgcagggat tatcccgccc aacccttcag aactactcag
     1021 ttcctaccgt ttcaaggatc tcatgaccgg attatttcct atgtattcat tcattattat
     1081 tgattcgcct cctgtgctcg gattgtccga cagccttctc ctgagcaccc tcatcgacgg
     1141 ggtaatcatg gtaacgagag caggtaatac cccaaaggat gcggcgatac aggcgagaaa
     1201 actcctgcag ggcgtgaacg ctaaaatcct tggtgttgtt ctcaacggta ttacggaagc
     1261 ggacctcaaa tacggctcat attcctatta ctattcaaac tactacggag atggatacga
     1321 tagtcacaag gaaaaaaaga agaggagagc cacggaccac gcttaatgtt tctttcaggg
     1381 tagtcttaac cattgtctcg attaccgtaa cgttagccct tgtacggccc gcgttatctg
     1441 tatattactc gcagaagatt tctgaacttg ataaggaaag actggttcgt gcctcgcgaa
     1501 tcacccctga agatgcctgg tatcaatatt tattgggttt tttggactat gccgctcatg
     1561 accgaccagg aatagagagg gcgatcggac attacctcct ctcattaaag agaaacccta
     1621 ccgacagcca tgcatggctc gccattgcca aagcatacag agatattgac agaaaggatg
     1681 acgcgagcta tgccctgaga aaagcatatt acctggataa gaacaccccc gatgtgattt
     1741 gggaattagc actctttttg ttgcttgaga acaaacagga cgaagccctt cctctgctca
     1801 ggagatacat tactttggtt cccgaggagc aggaaaatgt ctacaccctc tgctatatga
     1861 tgagagtcaa gcccgtgagt attctcaatg acctcgtgcc cgccgattac tctttttaca
     1921 accgctatct tgcttttctt attggggacg gactccttaa cgaatccacc gaagtgtgga
     1981 agcggataaa gacatataat cccgaaagga gcgaatatct gaaatattgc gacttcctca
     2041 tcgcctccgg agaggtgggc gagggattgg ctctttggga cgattttgcc aaaaggttcc
     2101 tcgatgtcaa ggacagacct gaaggtgaga tactctggaa cggtgatttt gaactgccca
     2161 ttgagaacgc aggatttgac tggaagatcg ggaaagctga tggcgtaagg atacacagag
     2221 accaggatat taaatggaca gggtttgcct cattaagcat aaattttaat ggaaacacaa
     2281 acccgggaat ctctttggcc caacagatcg tccctgtaca tccagggcag aaatataagc
     2341 tgtcaggaca tataaggacc gaaaagatca cgacgcaaaa tgggatagtt tttgaggcat
     2401 cgaactacct ttgtgattcc tttgtaaaaa agacagagcc tgtcactggg acaaccctat
     2461 ggaaaaagat cgaactcgaa ttcaccacgc ctcagagatg taaggcaata accattgccg
     2521 tgaagaggga acggtcagag aaatttgata acaagataag cggtgatgca tggatagatt
     2581 ccctgtcaat gactcagata aaacccaagt aatcttcaag aatgcgctgg aatttattgg
     2641 cgcggtcagg gccggtattt cccgggagcg tttctgcacg tatgctgttc ggttctctga
     2701 tgcattcatt gacatgctca atgacaccga ggaggcacgg aactttttgt catcaagggg
     2761 gatcagcact gactatgtgc agagcaatgt aaagacaatc ttcgcaagac tgcatctaaa
     2821 caaggagaat gatcactatt tgaccctcgc attgcctcgc accgctactg aggcgcaaat
     2881 acataaacgg tggaaagagt taatgcgcat ctaccatccc gacagaaata gcgaagaaga
     2941 cgcggcaatc tgcgccaagt ggattaatga agcatacagc atcctcaaga accccgggaa
     3001 gaggatggag tatgacagaa aaacagcaaa gaccgttgag tctcacttca ctcgccggag
     3061 aagtggagta atccattcaa ataaatcagg gagacgcctg ttcatttcac ctgaggtgag
     3121 gagaatggtt ccaaagctca taatagcttc ttgtatagcg gtctcctgca tcgtcctatt
     3181 ggtcatgttc ttaagaaaca gacttgaagt atacacgtat caggcctctg ctcttcctga
     3241 tcaattcatg caagatgccg gtcaggctca cagaacgccg gatattgaaa tgggagaccc
     3301 caaaaaaaag gaggagagca acgtgatggg ctttcagcct gccgaacctc cggggcaaga
     3361 caccaaaacg tcgacaggcg ctcttcccga aaagtcccaa aaggataagg agaccgaagg
     3421 agcacgcgtg aaggctttta acccgtccgg gtcccacacc cgatctgcag gctttgccac
     3481 actgaggacc tctccggccg ctcaggaaaa atccatggtc gatacaaagg caggcccaac
     3541 cgaaaaaaca gtccgtgaac aaatagaggg ttcggcattg gatactatgg tccagaccgc
     3601 gtcacctgat cagaatataa tgaaagatat ttcgcctgcc tctctgcaaa aaaagtcttc
     3661 cccggaacag atgagtgtgt caggtgaaat agcaaacgcg tcgcctcctg tgccaacccg
     3721 caaggaagat cacccgaatc tggagacaga ggtattt
//
LOCUS       DATKOU010000119         5919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3618662, whole genome shotgun sequence.
ACCESSION   DATKOU010000119 DATKOU010000000
VERSION     DATKOU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3618662"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VMU21_05505"
     CDS             <1..366
                     /locus_tag="VMU21_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77017.1"
                     /translation="MNICYLCHFKGVPPDRVLIGCPSCHSSPKQSIVVMGKTFSHGEA
                     LKAGYKCIQCHTEVLKGDGVTPKDKCYLCHVDRRERYINVKLIHEKHVTQKQIDCLWC
                     HTKIEHGKVAMAEKIPVRR"
     gene            complement(613..1443)
                     /locus_tag="VMU21_05510"
     CDS             complement(613..1443)
                     /locus_tag="VMU21_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77018.1"
                     /translation="MRKEQFDHISLFTVHTSRFTVLIVGKAVAKLECVANHFPGGNKM
                     KKVLFGTMLLALVIAVPIPTMAEVNVNIGIGFPLPPPIVFAAPPALVVLPDTYVYVVP
                     EIAADIFFWNGWWWRPWEGRWYRSHYYDRGWGYYNHVPRFYFDVDPGWRRYYSEHNWY
                     GHRWNYERIPHQRVQQNWKSWQNNRYWERKTTWGVQGYKPRPPQQRQELRQQRQQQYQ
                     QRPEVQQHQQQQRQQQQRQPHEKVQQQQRQEQRHQAQQPHSQPQGKPGRGEEEKQERR
                     "
     gene            complement(1470..2000)
                     /locus_tag="VMU21_05515"
     CDS             complement(1470..2000)
                     /locus_tag="VMU21_05515"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HUO77019.1"
                     /translation="MRLEYDFSKARKNPYARRLERQATIRLDEGRMGRAELKGSVRGR
                     AGKERMTRALVSAILACLILMAPCLVAGENTVVSDLRCDPYVISLGMTQSEVRRKCGE
                     PTNVQTWEEERVRRDFYKDIPVQSQEQLSQEPLLVKEHVIVEEAEYNFGPTRFMYYLR
                     FENGKLRQITVGNYGY"
     gene            complement(2032..2328)
                     /locus_tag="VMU21_05520"
     CDS             complement(2032..2328)
                     /locus_tag="VMU21_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77020.1"
                     /translation="MSYKLDFSDTALSKLEALGQSDPRKLRQIFMKILSLRRSTFTQD
                     SKKLEHFVYQGLSGYRVDQGEYRIIYAVDEKKKTVIIGPILNRNEDYKELKGLK"
     gene            complement(2318..2569)
                     /locus_tag="VMU21_05525"
     CDS             complement(2318..2569)
                     /locus_tag="VMU21_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HUO77021.1"
                     /translation="MKALSVAHAKTKFSEIVTSAEHQKERVVIEKRNKPVAVVIGYDD
                     YKKLEALEDIYESKLLEETIKKGKSYSLEEVAKRVRVEL"
     gene            2895..4388
                     /locus_tag="VMU21_05530"
     CDS             2895..4388
                     /locus_tag="VMU21_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77022.1"
                     /translation="MDHTPSSQELHHEIFGSVLDRTEMTPFLLLPAKDYATALSQAAQ
                     MMGNRGFDILGNAELQESEKERANRYIERVYRYFLDWLFPFVRKQLQGLAALHSANAD
                     LYRHFLDAIGEIEQVLMSTEFRRIVREDPRHLFLLASSRRYPRVFDGYKGSDMDVPAA
                     WQQIACSMLKMGHLIKSVEEDSQDINDYAQLGFFLEAQGQSLDDLYQYRWDNPKHIPD
                     NAPAQRAFVKVSTFFHKLKESLIFDEHKGCLVFNSGDGVSVDIADIKARLKSPESMFT
                     KLGKDVEGEAYDIRDILAISFILKSMDDTLKLFHALQKRGVILQENTASYSITQTLFE
                     SPQSMREAVRRLMVSLAQSGGSDKMPGEEDLLSNARTFYEALSVNAARNLHSSLGHRK
                     FQCKIAFSLPIHHTSDTNEIMIPGTRVYTMRDRVRKKTQQHTLGVELRISDEQSWRAS
                     ERKGDSHHDAYKFRQLVSVMNRVFFNRFQVSEESLAQLRRDQGALFL"
     gene            complement(4802..5707)
                     /locus_tag="VMU21_05535"
     CDS             complement(4802..5707)
                     /locus_tag="VMU21_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HUO77023.1"
                     /translation="MEDNRVEIDRDICNGCGACVTVCPTGTLSLIEGKAAVSGGESIS
                     CGHCEAVCPQEAIRVTAIDEEMSRYKTFTLEKQCLPHGEYNTSLLVQLMASRRSCRSF
                     TRQPVDRAMLEDLVKIGITAPSGTNSQSWTFTILPTSKAVASFAEHIASYFGRLNSTA
                     EKTILRVFLKLIGKGELDAYYHGYYRKVKEALEEWHGSGKDRLFHGSTAAILVGSKPG
                     ASCPAEDALLATQNILLAAHSMGLGSCLIGYAVAAMKKDPSIMQSVGIPSEEEIHAVI
                     ALGYPDEVYQRIAGRKKVTPRYFEG"
BASE COUNT         1381 a         1613 c         1431 g         1494 t
ORIGIN      
        1 atgaatatat gctacctctg ccatttcaag ggagtccccc cagatcgtgt gctcatcggt
       61 tgcccctcct gccattcttc tcccaagcag tctattgtgg ttatgggaaa gaccttctcc
      121 cacggcgaag cgttaaaggc agggtacaaa tgcattcagt gccacacgga agtattgaag
      181 ggtgacggcg tgaccccaaa ggacaagtgt tatctctgtc atgtcgacag aagggaaaga
      241 tacattaacg ttaaactgat tcatgaaaaa catgtgactc agaagcagat agactgcctg
      301 tggtgtcata caaagataga gcatggaaag gtagctatgg cagaaaagat acctgtgcgc
      361 aggtaatgtc cttgccatgg ggaagaaaag gaatggaatt cactaagtgg gggacggctt
      421 tctttggcgt ccctcctgct aaagcttctt ttccgcaaca atgaaaacaa tcattttgag
      481 cgaatgcggc tcaaaatagt agtaactggg ttcctggtat catacgccaa gtgcccgaaa
      541 tgagcaagag tatgacacct gaccagaact ccattcggta acgcgtcatc tgtgtgagca
      601 ccttttatcc tcctacctcc tctcctgttt ttcttcctcc cctcttccag gttttccctg
      661 aggctgagag tgtggttgct gagcctgatg tctctgctct tgtctttgct gctgctgaac
      721 tttctcgtga ggttgtcttt gttgctgctg tctctgttgc tgctgatgtt gctgaacctc
      781 aggcctctgt tgatattgct gttgcctttg ttgccttagt tcctgtctct gttgtggtgg
      841 tcggggttta taaccctgga cgccccaggt tgttttcctt tcccagtatc gattattctg
      901 ccagctcttc cagttctgtt gcacccgctg atgaggaatc cgttcatagt tccatcggtg
      961 cccgtaccaa ttatgctcac tgtagtatcg tctccacccc ggatctacat caaaataaaa
     1021 gcggggaaca tggttataat aaccccagcc ccggtcataa taatgtgagc gataccaacg
     1081 accttcccat ggacgccacc accaaccatt ccagaagaag atgtccgcgg ctatctcagg
     1141 gacaacgtag acatacgtgt caggcagcac taccaacgct ggtggtgcgg caaatacgat
     1201 gggcggcggc agaggaaatc caattccaat atttacattt acctctgcca tggtcgggat
     1261 aggaactgca attaccaacg ccaaaagcat tgttccgaaa agcacctttt tcattttgtt
     1321 gcctcctgga aaatgattag cgacacattc caatttcgcg acagcctttc ccacaatcaa
     1381 gaccgtaaag cgtgaagtgt gaaccgtaaa aagtgaaata tgatcgaact gctctttcct
     1441 cacctgcact cctcttattc cgcccgtctt taataaccgt agttcccgac ggttatttgt
     1501 ctgagtttgc cgttttcgaa tctgaggtag tacatgaatc tggttggacc gaaattgtac
     1561 tccgcctcct caacaatgac gtgttctttg acgagcaacg gctcctggga aagctgttcc
     1621 tgggactgaa cagggatatc cttatagaag tccctcctga cgcgctcttc ttcccatgtc
     1681 tggacgttcg tcggctcgcc gcacttcctc cgaacctctg actgagtcat tcccaaagag
     1741 atcacataag ggtcacatct gagatccgaa acaacggtat tctcgccggc cacaaggcag
     1801 ggtgccatca gaataaggca ggccaggatt gcactcacaa gggcccttgt cattctctcc
     1861 ttccctgctc tcccccgcac ggatcctttc agttctgccc gacccatcct gccctcgtcc
     1921 aaacggatgg tagcctgacg ctccagtctc cgggcatagg ggtttttccg tgccttagaa
     1981 aaatcgtatt ccaatctcac ttcatcacct cttccaatac agaactgtat actatttcag
     2041 ccccttgagc tctttgtagt cttcattacg gttaaggatg gggccgatta tcaccgtttt
     2101 cttcttctca tccacagcat aaatgatccg gtactcaccc tggtccactc tgtagccgct
     2161 taagccctga tagacgaagt gttcaagttt tttggaatct tgagtgaaag tcgaccttct
     2221 cagggacaat atcttcatga atatctgccg aagcttcctg gggtcagatt gtcccagggc
     2281 ttcgagcttt gacaacgcag tatcagaaaa atcaagctta taactcaacc ctcacccgct
     2341 tcgccacttc ctcaagagaa taggacttgc ccttctttat cgtttcttca agaagtttgg
     2401 attcataaat atcctcaagt gcttcgagct tcttgtaatc atcgtatccg atcactacgg
     2461 cgaccggttt gttcctcttt tcaattacca ccctttcctt ctgatgttcc gcgctcgtaa
     2521 ctatctcgga aaattttgtt ttcgcgtgtg ccacacttaa tgccttcatg gtgatacctc
     2581 ctgtgggcat tatagtcata ttggtcattt tagtcaataa ggccataata catgaaccgt
     2641 attgtaccgg ataatttttc agtgtagcat aacaggtgac gaccacatgg cctcatcccc
     2701 gcgacactca accatgccgg ctggtaccag tatccctcaa ttattgcaac atatttgaag
     2761 tgtcggtttt cagatgaagg ctgtcccgcc atctccaccc ctccaattaa tttccatagg
     2821 ctataataga gataaggttc tgcagactgc cggcggaatg tatccgtcgg tgagctgcct
     2881 taccaagtat gcatatggac catactccat cgagccagga actccaccac gagatcttcg
     2941 gttcagtcct tgacaggacc gagatgacgc ccttcctctt gctgccggcg aaggattacg
     3001 caacagccct ttcccaggcc gctcagatga tgggcaaccg cgggttcgac attctcggca
     3061 acgctgaact ccaggagagt gagaaggaac gggcaaacag gtatatcgag cgcgtgtaca
     3121 ggtacttcct cgactggctt ttcccctttg tgagaaagca actgcaagga ctcgccgccc
     3181 tgcactccgc gaacgccgac ctgtaccgtc actttctgga tgccatcggg gagatcgagc
     3241 aggtcctgat gagcacggag ttccggcgga tcgtacgcga agacccccgg catctcttcc
     3301 tcctcgcctc atcgcggaga tatccgcgcg ttttcgacgg gtataagggc agcgacatgg
     3361 acgtcccggc cgcgtggcag cagatcgcct gttccatgct caagatggga caccttatca
     3421 aatcagtgga agaggacagc caggatatca acgattacgc ccagctgggc ttttttctgg
     3481 aggcgcaggg gcagagcctt gacgacctgt atcagtatcg ctgggacaac ccgaaacaca
     3541 tccccgacaa tgcgcctgcc cagagggcct ttgtgaaggt ctcgacgttt tttcacaagt
     3601 tgaaggagtc gctgatattt gacgaacaca aggggtgcct tgtctttaac agcggggacg
     3661 gggtatccgt cgatatcgcc gatatcaagg ccaggctcaa gagccccgag agcatgttca
     3721 ccaaactcgg caaagacgtg gagggagagg cgtatgacat ccgggacatt ctggcaatca
     3781 gctttatcct gaagagcatg gacgacacgc tgaagctctt ccatgcactc cagaagaggg
     3841 gcgtgatcct gcaggaaaac accgcctctt attcaatcac acagaccctt ttcgagagcc
     3901 ctcaaagcat gcgggaggcg gtgaggcggc tgatggtcag ccttgcgcag agcggtggca
     3961 gcgacaaaat gccgggggaa gaagatctcc tctccaatgc caggacgttc tacgaggccc
     4021 tgagtgtaaa cgctgccaga aacctgcatt cttcgcttgg acaccggaag ttccagtgca
     4081 agatcgcctt ctccctcccg atccaccaca cttcggatac gaacgagatc atgatcccgg
     4141 gcacgcgggt ctacacgatg cgggaccggg tccgcaagaa gacccagcag cacacgctgg
     4201 gggtggagct gcgcatatcc gacgagcaga gttggcgcgc ttccgaacga aagggagact
     4261 cccaccatga tgcgtacaag ttccgccagc tggtctcggt gatgaacagg gtctttttca
     4321 acaggttcca ggtgtctgaa gaaagccttg ctcagttgag aagagaccag ggagccctct
     4381 tcctttgagc ggggagttcc cccatgagaa tagaccgcat gaagaatcca agactaaccc
     4441 ccatgaccat tccgcctgtg ttttactgct aataaacctc ccggtttttg tctgacagaa
     4501 atggtcgcgt atgtcaccaa gaagatgaaa agggctttat cccttccctt agcgcttaat
     4561 tatagatcga agcataacgt ctgaagcggc accaagtcat aagcaacggg ttgtgaggcg
     4621 tttggttcaa caagcaggaa tcatcacgaa ctgaaagaaa gtcgatatta ctttcggaaa
     4681 tcagcattct ctgcgggagt gctgaggtgt cacttatcag gatcctcttg taccggacaa
     4741 gttaggtacc gatctgcggg tgcggcagac tgcccgaaat gcgaagtctt tctcccggct
     4801 gctatccctc gaaatatcgc ggtgtcacct tcttgcgccc tgctatccgc tgatagacct
     4861 cgtcgggata tccgagggct atcactgcat gaatctcttc ttctgacgga attccgacag
     4921 actgcatgat cgaggggtct ttcttcattg ctgcaacggc atatccgatg agacaagaac
     4981 cgagtcccat gctgtgggcc gcgagcagga tattctgtgt tgcgagcagg gcatcctcag
     5041 ccggacagct tgctcccggc ttagatccga caagaatggc ggccgtgctg ccgtgaaaga
     5101 gccggtcttt ccctgatcca tgccattctt caagcgcctc cttcaccttc cggtaatagc
     5161 cgtggtaata ggcatcgagt tcccctttgc ctatcagctt gaggaacaca cggagtatgg
     5221 tcttttcggc agtagagttt aatcttccga aataggaagc gatgtgttct gcaaaggatg
     5281 ccaccgcctt acttgtcggc agaatggtga aagtccagga ctgactgttg gtcccggacg
     5341 gtgcggtgat gccgatcttt acgaggtctt cgagcatagc ccggtccact ggctgcctgg
     5401 tgaaggaacg gcaggaccgg cgtgaggcca taagctgcac caagagagat gtattgtatt
     5461 ccccgtgagg aaggcactgt ttctcgaggg tgaacgtctt ataccgtgac atctcttcat
     5521 caattgcggt gacacggatc gcctcctggg gacatactgc ctcgcaatgt ccgcaagaga
     5581 tcgactcccc tcccgagaca gccgcctttc cttctatgag tgagagggtc cctgtcgggc
     5641 acaccgtgac gcatgccccg cagccgttgc aaatatccct gtcgatttct accctgttgt
     5701 cttccattcg cgtctcctca ataaccattt ataagtttat ttctatgata ccactgccat
     5761 caaatcattt ctcaccctcc ttttctctga tgaagaacca gggttatcag gcggaattct
     5821 cccgccattt caatgtagcc tccaacagta agaaatgtct tcccgccagt gcggccgggc
     5881 ggcatagttc cgacattatg tctcgcttct taaccgaaa
//
LOCUS       DATKOU010000120        11652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5160244, whole genome shotgun sequence.
ACCESSION   DATKOU010000120 DATKOU010000000
VERSION     DATKOU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5160244"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VMU21_05540"
     CDS             <1..1251
                     /locus_tag="VMU21_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO77024.1"
                     /translation="SEREIIAKIREVKEQIEKTKIESEKAEREGDLSKAAELRYGRLL
                     ELQKALDTQNKRLADAQSRRKMLKEEVDEEDIAEVVSKWTGIPVSRMMEGEIQKLLHM
                     EERLRQRVVGQDDAIIAVANSVRRARAGIQDMNRPIGSFIFLGPTGVGKTELAKALAE
                     FLFDDENAMQRIDMSEYQERHTISRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILFDE
                     VEKAHPEVFSLLLQLLDDGRLTDGHGRTVDFRNTVVIMTSNIGSTHIQEILLDRERRP
                     AAYWGKSNDEELKESVMNDLKAFFKPEFLNRVDEIIIFNPLSKDLLKQIVDIQVNRMK
                     GYLKEKRIDLVLTDAAKERLGEIGYDPVYGARPLKRAIQREILNPLAMKLLDGTFKEG
                     DMVEVDFEDSQPIFRKRTEAEVAV"
     gene            1383..1844
                     /locus_tag="VMU21_05545"
     CDS             1383..1844
                     /locus_tag="VMU21_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUO77025.1"
                     /translation="MSIVKWSPMKELEDMRRDMERLFEEVFEPSHRRRHWWPKSPEGG
                     VVVPNIDMYDRKTEIVVKAELPGVERDKIDLTITRDAITIKGEIRREEEIKEEDYYSR
                     EISYGNFTRSVTLPAEVESEKAKATFKNGILEIVLPKKEEAKPKEIKVEVS"
     gene            complement(2035..3039)
                     /locus_tag="VMU21_05550"
     CDS             complement(2035..3039)
                     /locus_tag="VMU21_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77026.1"
                     /translation="MIKSLSTTGIGSLPYRDPEEACNLVFRTFDIPFWPQLPRLSFLE
                     WMIPQYSEGMPFVQVDKETETIVVNRNVSDELERFYESCKEACRIAISDDYARGLHAF
                     LRRIRGRHVESIKGQVTGPLTFTLGLKDREGRLIYFDEELREISLMLLQAKTRWQIDQ
                     LRSYADNVLIFLDEPIFSAIGSSTYLGVSRDETLRLLAEMTGTIMDAGGIPGIHCCAN
                     ADWTLAIESGAKVISFDAYGYFENLAMYHNEIKVFLEKGGYLAWGIVPTTDAIEKETP
                     ESVIDKFLSRLESLSAYIPKNLLLSQIILTPSCGTGSRSIEETIKIFQLLMRLKEACG
                     "
     gene            3109..3342
                     /locus_tag="VMU21_05555"
     CDS             3109..3342
                     /locus_tag="VMU21_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77027.1"
                     /translation="MKPRLYLKVGDTVRHIRYYAWGKGVVVEESHSTLAGGFCFVRVR
                     FEDGTERSFINDLDNECCCYYAGLRAIQYNVLG"
     gene            3415..4428
                     /locus_tag="VMU21_05560"
     CDS             3415..4428
                     /locus_tag="VMU21_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO77028.1"
                     /translation="MTPLHGSAIQERPSALIVDDLWSNLELLEAIFSNAGFEVFSARD
                     AYSALSIFESHKIDIAIVDIMMPGMDGYELCKQLKSLTEKYFFPVILLTALTDRESRI
                     TGLQCGADDFISKPFDTTELIIKVRSLIKLKTLQDELEHSENVILTLAVAMEARDPYT
                     RGHSTRVGDLSKSFASFLGFGKREQEVMRKAGILHDIGKIGVTTDLLCKEGKLTCDEL
                     EAIKRHTLMGEEICRPLVSMRGILPIIRNHHERWDGKGFPDGLQGEEIPVNARILSIA
                     DSFDAMISQRPYRNGRSMDLVIEVFKNEKHAGQWDPSLLDQFVEMMDAMHDERISGWS
                     SLQ"
     gene            4410..7421
                     /locus_tag="VMU21_05565"
     CDS             4410..7421
                     /locus_tag="VMU21_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77029.1"
                     /translation="MVKLAIAGGNIAGSTVMSMMRGDPDIVLVGVFERDTEAPGAVLA
                     QKWGIRLFDDIAALASKARPEILINVTGETNISEQVRVLDPHIEVIDASGARFLWKAL
                     EKHKKAMIELYKTMEDEKRILFITENVSLSENFDEFIGFVLDKTLEVTDCPAGSIATA
                     EGDEMHLLIAKGLSKRFAENRIWKIMPGGLTDRVLRKKEITAIHDISTAHYINNPTLV
                     REGITAMLVCPILLNGIVQGIIYLDDFKARQFSERQKRAVSLIARITGLIFERVNLLR
                     NITAQREELSHLHENFDKKVKDRIEELEKLNKELERASQHKSRFIANMSHELRTPLNS
                     IIGFSDVLLEKTFGELTPNQERYAKNIHSSGRHLLELVNNVLDIAKIEAGKYEMSYET
                     FPVVDMMSEIFAVMKPMADKKAIALKLSIGDDVDAITADMVKLKQVCYNLLSNAIKFT
                     PEGGKVCVGVERRTGRGEFEHQKFTTFSVSDTGVGISPDALERIFDEFEQADSTFSKE
                     YGGAGLGLALTRKLVELHGGSITVESKLGEGSTFTASLPLISDVHEKPADEIEAVNLN
                     FPWMNEKAPLILVVEDDPASAELLTIHLTQAGYKVAHAFDGEEAIQKAVSLRPFAILL
                     DIMLPKKDGWEVLQSFKSNELTSHIPVLIHSIIDNKDLAFALGATDYLLKPLDKDALL
                     EKLHGLSTLRGRKYPTTILIIDADGDCESLKEALNPQEFLISSAREGKRGIELATAMR
                     PDVVLLDFELPDMLGFDVVRELKENPSTRNIPIFILTEKDISVEGRLSLMGKIERIIR
                     KHKFEVKELIDHITDIEVLYPSRAGLVDELTGIFSHRYFHLRLAQEIERASRYKQPLN
                     LMLLDVDLFGRYLRENGEQNGNSVLKTISELLTKNIRGSDVVVRYGGDAFAIVLPNTV
                     PSAALSLGNRFNAIIRNYPFPHKETQPKGRMTVSVGMAFLDGQSPEELIICCEKALAQ
                     AIKKGGDRVEMYSKEGEEEKQASPKVMDSQG"
     gene            7452..7940
                     /locus_tag="VMU21_05570"
     CDS             7452..7940
                     /locus_tag="VMU21_05570"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HUO77030.1"
                     /translation="MSRKSHIFFDTLHTPLGILFFVFSGERLAEIRFENEAPPYSRGK
                     APEAFTRQLRDYFDGTLREFHQSIVFLHGTDFEQGVWLSLKDIPYGETRAYKWLAERI
                     GNPKAVRAVGRALSRNPLPIVLPCHRVIESDGSIGGYSSGVERKRRLLDMEYYYSVGK
                     ER"
     gene            complement(8152..9096)
                     /locus_tag="VMU21_05575"
     CDS             complement(8152..9096)
                     /locus_tag="VMU21_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUO77031.1"
                     /translation="MHKLAQNQMIENKSLQVAIEVLAKSIARWTDKPDRIMTAIPGLS
                     LFRRDEPTQPASVMYEPSICLTTQGTKRILLGDDTYVYDQHHFLITSVDLPTVVQIIK
                     ASREKPFLGLILNLDQRGISQLMVDSNLPPPRPQQTSRGMATGEVTFPLLAAFQRLID
                     LLDEPKDIPILAPIIQRELFYRLLVGDQGVRLRQMASAGSQSQQIARAIYWLKGHYTQ
                     PLRIDVLATQVNMSTSTFHHHFREVTAMSPLQYQKWLRLNEARRLMLTENQDATTAAF
                     QVGYESPSQFSREYSRLFGNSPLRDITNLRQITANEIA"
     gene            9329..10315
                     /locus_tag="VMU21_05580"
     CDS             9329..10315
                     /locus_tag="VMU21_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUO77032.1"
                     /translation="MQKRKLGNSGLEVSALGLGCMGMSIAYGQPGDKQEMIALIRKAV
                     ERGVTFFDTAELYGPYTNEELVGEALAPFRGKVVIATKFGIKLDAGGQQVQDSRPERI
                     RQSLDGSLKRLKVDAIDLYYQHRVDPDVPIEDVAGAIKDLIREGKVRHFGLSEPGVKT
                     IRRAHAVQPVTAIQSEYSLWWRRPEAELLPTIEELGIGLVPFSPLGKGFLTGKIDEKT
                     TFDKSDFRNIVPRFTPEARKTNQALVDLLRNISDRKKATPAQIALAWLLAQEPWIVPI
                     PGTKKLERLEENIGAVNVELTPDDLREIDSAASKVTIQGDRYPEALEKRTGL"
     gene            10401..11459
                     /locus_tag="VMU21_05585"
     CDS             10401..11459
                     /locus_tag="VMU21_05585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HUO77033.1"
                     /translation="MNVRGYAAQSAKDALAPYRFERRDQRPDDVAIEILYCGVCHSDL
                     HTVRNDWASWFPTTYPVVPGHEIIGRVTSVGKDVKRFKPGDHAGVGCMVDSCQKCTAC
                     EQGHEQYCSEIATFTYNHVDRRDKMPTYGGYSEKIVVSDKFVLKIPDGLDLKGAAPLL
                     CAGITTWSPLRHWKVEKGSKVAVVGLGGLGHMALKLANALGADVTLFTRSAGKEKDAR
                     RLGAHNVVLSTDKGQMTSVQRRFDLIIDTVPYVHDLNHYLPSLTINGTLVLLGFLGGL
                     DPILSTVPLVMGRRSVAGSVIGGIAETQEMLDFCGKHGITSDIEVIKIQDINKAYERM
                     LKSDVKYRFVIDMASLKV"
BASE COUNT         3116 a         2677 c         3175 g         2684 t
ORIGIN      
        1 agcgaaaggg agatcattgc aaagataagg gaagtcaagg agcagatcga gaagacaaag
       61 atagagtccg aaaaggctga aagggagggt gatctttcga aggctgcaga gcttcggtac
      121 gggaggctcc ttgagcttca gaaggccctg gatacccaaa acaaacgact tgcagacgca
      181 cagtccagaa gaaagatgct taaagaagag gttgacgagg aggatattgc tgaggtcgtc
      241 tccaagtgga caggcatccc tgtctcaagg atgatggagg gcgagatcca gaagcttctc
      301 cacatggaag agcgactgag gcagagggtt gtgggacagg atgacgccat cattgcggtg
      361 gccaactcgg tgaggcgtgc acgggcaggc atccaggaca tgaacagacc tattggttcg
      421 ttcatctttc tcggacccac tggtgtgggg aagaccgagc ttgccaaggc acttgctgag
      481 ttcttgtttg atgacgaaaa tgcgatgcag aggatcgaca tgtccgaata tcaggagagg
      541 cacaccatat cgaggctgat aggggcgccc cccggctacg tgggatatga ggaagggggc
      601 cagctcaccg aggcggtaag gagaaggccg tattccgtaa tcctctttga tgaggtggaa
      661 aaggcccatc ccgaagtatt cagtctcctc ttgcagctcc ttgatgatgg aaggcttact
      721 gatggccatg ggaggaccgt ggacttcagg aacaccgtgg taatcatgac ctccaatatc
      781 gggagcacac atatccagga gattctcctt gatcgggaaa gacggccggc tgcctactgg
      841 ggaaaatcca atgatgagga gcttaaggag agtgtgatga acgatctcaa ggcatttttc
      901 aagccggagt tcctcaacag ggttgatgag atcatcatat ttaatcccct ttccaaggac
      961 ctccttaagc agattgtgga tatccaggtg aacaggatga aaggatatct caaagagaag
     1021 aggattgatc tggtgctcac ggacgcggca aaggagaggc tgggtgagat cggctatgac
     1081 cctgtgtatg gcgcacggcc tctcaagcgt gcgatacaga gggagattct caatcccctt
     1141 gcaatgaagc ttctggacgg aaccttcaag gagggtgaca tggttgaggt ggattttgag
     1201 gacagtcagc ccatcttcag gaaacgtaca gaggcggaag tggctgtttg aggcaagagt
     1261 gagcttacaa tgattcatcg aagctgaaag aagtgcacga ggttagtgag aaaggccctt
     1321 ggcctataac gaaccaatca agtattcctg ttgatggaaa gcaggaagag gaggtaaatg
     1381 atatgtcaat tgtaaaatgg tctcccatga aagaactgga agatatgaga agggatatgg
     1441 aaaggctttt tgaagaggta ttcgaaccgt cgcacaggcg gagacattgg tggccgaagt
     1501 ctcctgaggg aggcgtagtg gttcccaaca tcgacatgta tgacaggaag accgaaatcg
     1561 tggtgaaagc agagcttcct ggtgtcgaaa gggacaagat cgacctcacc atcaccaggg
     1621 atgctattac cataaagggt gaaatcagga gggaagagga gattaaggaa gaagactact
     1681 attcacggga gataagttac ggaaatttca cgaggagcgt tacactcccc gcagaagtgg
     1741 aaagcgaaaa ggcaaaggcc acattcaaaa atggcatcct cgagattgtc cttcccaaga
     1801 aggaagaggc aaaacccaag gaaataaagg tggaagtatc gtaaagctga gagcggtgac
     1861 ctgaaatatt gctccaaaaa taggaatacg gtcagggtta tttagtagtg agaggagcaa
     1921 actggcaaag aattgagtat cccttgagat tcttgatttc ccgacctcat ctctttggta
     1981 gtaatgcaga cattgccctt gtgtcaagcc ttcctggtga atttggagtt tcctttatcc
     2041 gcacgcttct ttgagcctca tgaggagctg gaatatcttt atggtttcct ctatgctcct
     2101 tgacccggtt ccgcaggacg gagtaaggat aatttgtgaa agcagcaggt ttttgggaat
     2161 gtatgctgat aagctctcta gcctgcttag gaacttatcg ataacagatt caggggtctc
     2221 tttttcgatg gcatcagtag tgggaacaat accccatgca aggtagccac ccttttcaag
     2281 gaagacttta atctcgttat gatacatggc aagattttcg aaatagccat aggcatcgaa
     2341 gcttatgacc tttgcaccgc tttctatggc aagagtccaa tccgcgttgg cgcaacaatg
     2401 gattcccggg attccgcctg cgtccatgat tgtaccggtc atttctgcaa gtaaccgcaa
     2461 ggtttcatcc ctgcttaccc caaggtaggt actgctccca atagcagaga agatcggttc
     2521 atccaggaaa atcagtacat tgtcggcata tgatctcagc tggtcaatct gccatcttgt
     2581 ctttgcctgc aaaagcatta acgaaatctc gcgaagttcc tcatcaaagt atatgagtct
     2641 gccttctctg tcctttaatc caagggtaaa ggtgagaggc cctgttacct gtcccttgat
     2701 ggattcaacg tgtcttcccc tgattcttct aagaaatgca tgaagtcccc tcgcataatc
     2761 atccgatatg gcgatcctgc atgcttcttt gcatgattca tagaatctct caagctcatc
     2821 actcacattc ctgttgacaa ctattgtttc tgtctcttta tcgacctgaa caaatggcat
     2881 accctcagaa tactggggta tcatccattc caggaacgat aatcggggaa gctgcggcca
     2941 aaaaggtatg tcaaaggttc tgaacacaag attgcacgcc tcttccgggt cacgataggg
     3001 aagactgcct atccctgttg tggaaaggga tttaatcact caaaaatcat acatgaaagg
     3061 aaggggaaag gcaatgagtt tgaagagtac gggatggtat aataaaagat gaaaccccga
     3121 ttgtatctga aggtgggcga tacggttcgc cacataaggt attatgcttg gggaaaaggt
     3181 gtggtcgttg aagagagcca ctctaccctt gcgggagggt tctgttttgt aagggtacgt
     3241 tttgaggacg gtacggaaag gtcgtttatc aatgatcttg ataatgagtg ttgctgttat
     3301 tatgcagggt taagggcaat ccaatataat gttttaggtt aggttttcat ttataactat
     3361 aaatattaaa agaaaaatct tgacaaatgc atctgggaat gctttaattc ccttatgacc
     3421 cccttacatg gttcagccat tcaggagagg ccatctgctc ttattgttga tgacctctgg
     3481 tctaacctgg aactccttga ggctatcttc agcaatgccg ggtttgaggt tttcagcgcc
     3541 cgggatgcat attcagccct ttccatattc gaatcccata agatcgacat tgcaatcgtt
     3601 gacatcatga tgcccggcat ggacgggtat gaactctgca agcaactgaa gtcccttacc
     3661 gagaaatatt tctttcccgt gatactcctc actgccctca ccgataggga gagcaggata
     3721 accggcctcc agtgcggtgc tgatgatttt atcagcaaac cctttgatac cacagagctt
     3781 atcatcaaag tgagatcatt aatcaagctg aagaccctcc aggatgagct ggaacattcc
     3841 gaaaatgtga tactcacgct tgcggtggcc atggaagcaa gggatcccta tacgagagga
     3901 cactccacaa gggtgggaga cctttcgaag tcctttgcat catttcttgg ttttgggaag
     3961 agggaacagg aagtcatgag aaaggcaggg atactccatg atataggcaa aataggggta
     4021 accacagacc tgctctgcaa ggagggaaaa ctcacgtgtg acgagttaga ggccatcaag
     4081 cgccacaccc tcatgggaga ggagatctgc aggcccctcg tttccatgcg ggggatactc
     4141 cctatcataa gaaaccacca tgagaggtgg gatgggaagg ggtttcctga cggtttgcag
     4201 ggtgaggaga tacccgttaa cgcaaggata ctgtccattg cggattcctt tgacgccatg
     4261 atttcacaaa ggccttacag gaatggaaga tccatggatc tggttattga ggtgttcaag
     4321 aatgagaagc acgccgggca gtgggaccct tctctcctgg atcaatttgt cgagatgatg
     4381 gacgccatgc acgacgagag gatttcagga tggtcaagct tgcaatagcg ggaggaaaca
     4441 tcgccggcag cacggtaatg tccatgatga ggggggaccc ggatatcgtg ctcgtaggag
     4501 tttttgaaag ggatacagag gccccggggg cagtacttgc ccaaaaatgg ggcatccgtc
     4561 tctttgatga tattgctgcc cttgcttcaa aagccagacc ggaaatcctt attaacgtta
     4621 ccggcgagac aaatataagt gaacaggtga gggtattaga cccccacatt gaggttattg
     4681 atgcgtcagg cgccaggttt ctctggaagg ccctggagaa acataaaaaa gcgatgattg
     4741 agctttacaa gaccatggag gatgagaaaa ggattctctt cattacagag aatgtgtcct
     4801 tgtcagaaaa ctttgatgaa ttcatcggat tcgtgcttga taagaccttg gaggtaaccg
     4861 attgccctgc ggggagcatc gcaacagcgg aaggggatga gatgcatctt cttatagcaa
     4921 aagggctgtc caaacgtttt gcagagaaca ggatctggaa gatcatgcca ggggggttaa
     4981 ccgacagggt tctgagaaag aaggagatca ccgcgataca cgacatctcc acagcgcatt
     5041 atatcaataa tcccacgctc gttcgtgaag ggattaccgc aatgctcgta tgtcccatcc
     5101 tccttaacgg gattgtccag ggaataatat accttgatga tttcaaggca agacagttct
     5161 ccgagaggca gaaaagggct gtcagtctaa tcgccaggat aaccggcctc atctttgaga
     5221 gggtgaatct tctcagaaac atcacggcgc agagggaaga actctctcat ctccacgaaa
     5281 acttcgacaa aaaggtgaag gacaggatag aggagctgga aaagctgaat aaggagcttg
     5341 agcgtgcgag ccagcacaag agcagattta tcgcaaatat gagccatgag ctgagaacac
     5401 cccttaattc cattataggc ttttctgatg tcctgttgga gaaaaccttt ggtgaactga
     5461 cgccgaatca ggaacggtat gcgaagaaca tccattcgtc gggaagacat ctcctcgagc
     5521 tcgtgaacaa tgtgcttgac atcgccaaga tagaagcggg caagtacgag atgagctatg
     5581 agacattccc tgtagtggat atgatgagcg agattttcgc cgtaatgaag cccatggcag
     5641 acaaaaaggc catagccctc aaacttagca tcggggatga tgtggatgcc atcactgcag
     5701 atatggtgaa gctcaaacag gtgtgctata acttgctttc caacgccata aaattcaccc
     5761 ctgagggtgg taaggtgtgc gtgggtgtcg agcggaggac aggacgggga gagttcgaac
     5821 atcagaaatt caccacattt tccgtgagtg ataccggggt aggcatcagc cccgatgccc
     5881 tggaaaggat ttttgatgag ttcgagcagg cagacagcac attctccaag gaatatggag
     5941 gtgcgggcct gggccttgca ctcactcgta aactggtcga gctccacgga ggctcgatca
     6001 ctgtggagag taaactgggc gaaggaagta cgttcactgc ttctcttccg ctcatctctg
     6061 atgtgcacga gaaacccgct gatgagatag aggcagtgaa tctgaatttt ccatggatga
     6121 atgaaaaggc gcccctgatt ctggtagtgg aagacgaccc tgcaagtgcc gaactgctca
     6181 ccatacacct tacccaggca gggtataagg ttgcccatgc ctttgatggg gaagaggcga
     6241 tacaaaaggc tgtgtcactg aggccctttg caatcctcct tgatatcatg ctgcccaaga
     6301 aagacggctg ggaagtgctc cagtctttca agagcaacga actcacttcc catatccctg
     6361 tgctcatcca ttccatcatt gataacaagg acctcgcctt tgccttgggc gccaccgatt
     6421 atctgctgaa accccttgat aaagacgccc tcctcgagaa gctccacggg ctgagtacct
     6481 tgagggggag gaaatacccc accacaatac ttataattga cgcggatgga gactgtgaat
     6541 cgttgaagga agcgcttaat ccgcaggagt ttctcatatc ttcggcgagg gaggggaagc
     6601 ggggaattga actcgccact gcaatgaggc ctgatgtggt gctgctggac ttcgaactcc
     6661 ccgatatgct gggctttgac gtggtgaggg aactgaagga aaacccttcg acgaggaata
     6721 tccctatctt catccttacc gagaaggata tttcggtcga gggaagactg agcctcatgg
     6781 gaaagatcga gagaatcatc agaaaacata aattcgaggt aaaagaactc attgaccaca
     6841 tcacggatat agaggtgctc tatccttcga gggcaggact ggtggacgaa ttgaccggga
     6901 tcttcagcca ccgttatttt cacttgagac ttgcccagga gattgagagg gcgtcacgat
     6961 acaagcaacc attgaatctc atgctccttg atgtggatct ctttggtcgt tacctgagag
     7021 aaaacggtga gcagaatgga aatagtgtgc taaagacaat ttctgaatta ctcactaaga
     7081 acattagagg gtccgatgtg gtggtgagat atgggggaga tgcctttgcc attgttcttc
     7141 ccaatacggt cccttcagca gcactctctc tcggcaacag gtttaatgca atcatacgga
     7201 actacccttt cccccataaa gaaactcagc ccaaagggag gatgacggtg agtgtgggta
     7261 tggcgttcct cgatggccag agtcccgaag aactcatcat atgctgcgaa aaggcgctgg
     7321 ctcaagccat aaagaagggt ggtgacaggg tcgagatgta ctccaaagaa ggggaagaag
     7381 aaaagcaggc atctcccaag gttatggact ctcagggttg aaattctcgc ggtttttgaa
     7441 gataattgag cgtgagccgc aaatcccata ttttctttga cacgttacat acccctctgg
     7501 gtatcctctt cttcgtattt tccggtgagc gactggcgga aataaggttc gagaacgagg
     7561 ctcctcctta ctccagagga aaggcccccg aggcatttac aaggcaatta agggactact
     7621 ttgacgggac actcagggaa tttcatcaga gtattgtgtt ccttcacggt actgattttg
     7681 agcagggggt atggctctcc ctgaaggaca ttccttacgg agagacgagg gcctataaat
     7741 ggcttgcgga aagaatcggc aatccaaagg cagtaagagc ggtgggccgg gcgctcagca
     7801 ggaacccgtt acccattgtg cttccctgcc acagggttat cgagtcagac ggctccatcg
     7861 gcgggtattc gtcaggggtt gagagaaaga gaagacttct cgatatggaa tactactaca
     7921 gcgtgggaaa agaaagatag gcttctcttg gtcagcggtt ccgatgcaaa cctgttacct
     7981 cggtagggga accatcggct ttgccccgaa acatgcaccg aaatgccctt cctgaagtct
     8041 gcacaggaag acatcacgaa aattctatag cttacggaaa tcctcagcct gaaatgcaag
     8101 aatgccttgg cagcgagggg cagcccttat cattagaatt ggcaatgccg gttaagctat
     8161 ctcattagca gttatttgac gtaaattcgt gatatcgcgc aatggcgagt tgccaaacag
     8221 acggctgtat tctcggctaa attgggaggg gctctcatag ccgacctgaa acgccgcggt
     8281 tgtcgcatcc tgattttcgg tgagcatcaa ccgccgcgct tcgttcaaac gcagccattt
     8341 ctggtactgc agcgggctca tggccgtcac ttcccggaag tggtgatgga aagtcgatgt
     8401 gctcatattg acctgcgtcg caaggacatc gatacgcagc ggctgtgtgt agtggccctt
     8461 caaccaatag attgcccgcg caatctgttg actctgactt cccgccgacg ccatctggcg
     8521 caaccgtaca ccttgatcgc ccacaagtaa ccggtaaaaa agttcccgct gtatgatcgg
     8581 cgcaaggatc gggatgtcct tcggttcatc aagcaggtcg atcaaacgtt gaaaggcggc
     8641 gagcagcgga aatgtaacct cgccggtcgc cattccacgg ctcgtttgct gtggacgggg
     8701 gggaggcaga ttgctgtcca ccatcagttg tgaaatcccg cgctgatcaa ggttcaatat
     8761 aagccccagg aagggcttct cccggcttgc tttgatgatc tgcacaaccg tgggcagatc
     8821 caccgaagtg attagaaaat ggtgttggtc atacacgtac gtgtcgtccc cgagaagtat
     8881 gcgttttgta ccttgcgtag tcaagcaaat actcggttcg tacatgacgc tggccggctg
     8941 agtcggctcg tcccgccgaa aaagtgacaa tccggggatc gcagtcatga tccggtctgg
     9001 tttatcagtc catcgggcaa tactctttgc caaaacctca attgctactt gcagggattt
     9061 attctcaatc atttgattct gggccagctt atgcattaat gcctcctttt ctcaatgata
     9121 ccaagcttta cttcatcgta acaacaaatt tatcgtgggc tcgtacgatt aggcaagaaa
     9181 ttaacagaat tggtctaccc gactttggtt tttcagattt acaataaaat catgagcagg
     9241 gtggctgacg cctcgttagc cacatagaag acaatatgat acgatctgat aaggaaacat
     9301 tgcgtttgat aaccaaggag gataaacaat gcaaaaacgt aaattaggaa atagtggcct
     9361 ggaagtttcg gctctcgggc tcggttgcat ggggatgagc atagcttacg gtcagcctgg
     9421 agacaagcag gagatgatcg cccttatacg taaagccgtg gaacgaggcg ttacattctt
     9481 tgacaccgcc gagctttacg gcccctacac aaatgaagaa ctcgtaggcg aagctttggc
     9541 tccgttccgc gggaaggtgg ttatagccac caagttcggg atcaagcttg atgccggcgg
     9601 gcagcaggtc caggatagtc ggcccgagcg catcaggcag agcctcgatg gctcgctcaa
     9661 gcggctcaag gtcgatgcca tcgacctcta ctatcaacac cgtgttgacc cggatgtgcc
     9721 gatcgaagat gtggcgggag caataaaaga cctgatccgg gaaggcaagg tcaggcactt
     9781 cggtctttct gaaccaggag tgaaaacgat ccgccgcgcg cacgcagttc agccggtcac
     9841 tgcaatccag agtgaatact cgctgtggtg gagacgccct gaagcggaat tgttaccgac
     9901 gatcgaggaa ctcggaatcg ggctcgttcc atttagtcct cttggtaaag gcttcctcac
     9961 cgggaagatc gatgagaaga ccacgttcga taagtcagac ttccgcaaca tcgttcctcg
    10021 ctttacgcca gaggcccgga aaacgaatca ggcattggtc gatctgcttc gcaacatctc
    10081 agatcgaaag aaggcgacgc ctgctcagat agcactcgcc tggctgcttg cccaggagcc
    10141 gtggattgtt cccattcctg gcaccaagaa actggagcgc ctggaagaga acataggagc
    10201 agttaacgta gaacttactc cggatgacct tcgtgagatc gatagcgctg cctcaaaggt
    10261 cacgatccag ggagatcggt accccgaagc ccttgaaaaa agaaccggtc tctaagcagc
    10321 caaaacggag ggagacctcg ggagaggacg cccttaagta agaacgaaaa tttgttacca
    10381 tggaggaaaa agcaatgaca atgaatgtac gaggttacgc agcacagtct gccaaggatg
    10441 ccctggcccc atatcgtttt gagcgacgcg atcagcggcc tgatgacgtg gctatcgaga
    10501 tactctactg cggcgtatgc cattctgacc tgcataccgt ccgcaatgac tgggccagct
    10561 ggtttcccac tacgtacccg gtcgtgcctg gtcatgaaat catcggccgc gtaacaagcg
    10621 tcggaaagga tgtcaaacgc ttcaaaccgg gagatcatgc cggtgtcggc tgcatggtcg
    10681 attcgtgcca gaagtgcaca gcctgcgaac agggacatga gcagtattgc tcggagatcg
    10741 cgacattcac ctataaccat gttgaccgcc gcgacaagat gcctacctat ggcgggtatt
    10801 cagagaagat cgtggtatcg gacaagtttg tgctcaagat ccccgatggg ctggacctga
    10861 aaggggcagc gcctctcctg tgcgccggca tcaccacctg gtcaccgctc cgacactgga
    10921 aggtggaaaa aggcagcaag gttgcggttg tcggcctcgg cggactgggt cacatggcac
    10981 tgaagcttgc gaatgcgcta ggcgctgatg tcacgctgtt cacccgttca gcgggcaaag
    11041 agaaggatgc gcgccgtctg ggcgcccaca atgtcgtgct ctctaccgac aagggccaga
    11101 tgacatccgt gcagagacgg ttcgatctga taatcgatac cgtgccctat gtccatgatc
    11161 tcaaccacta tctgccgtca ctgacgataa acggcacgct ggtgctgctt gggtttctgg
    11221 gcggtctgga tccaatattg agcaccgttc cgttggtgat ggggcgcagg tcggttgcgg
    11281 ggtcagtcat cggcggtatt gccgagaccc aggagatgct cgatttttgc ggcaaacacg
    11341 gcatcacgtc cgatattgaa gtgatcaaga tccaggatat caataaagcg tatgaacgca
    11401 tgctgaaaag cgatgtgaag taccgttttg tgatcgacat ggcatcgctg aaagtttaat
    11461 gccgaggagg ttgtcatgaa gagtgtcacg gttttaatag gatccgggtc gataggtgtc
    11521 gcaatcgcac gcagagtgag cgctggaaaa cacattctgc ttgccgattt aaaaaaagag
    11581 aatgcggacg ccgcagccgc agttctaaat aacgcaggat ttgaagtcag cactgcgacc
    11641 gtcgatgtat cc
//
LOCUS       DATKOU010000121         8444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5547536, whole genome shotgun sequence.
ACCESSION   DATKOU010000121 DATKOU010000000
VERSION     DATKOU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5547536"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..844)
                     /locus_tag="VMU21_05590"
     CDS             complement(257..844)
                     /locus_tag="VMU21_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUO77034.1"
                     /translation="MNETRKCDFCGKDINAAYTICPLCGGHLRDKVEARPPACPRCKQ
                     PLDVHNAGGEEYDLCPRCGGLWLDRGEFHLLTREYDVYRDNFKDEYLREPLKDPVEYI
                     SCVRCGKLMNRKNFGKISGVIIDECGKHGVWLDAGEMEKIRHFIADGGLERAQDLEIE
                     KTRMELRDLATNVDQTAFTQKVIHFWNLKRWLFGG"
     gene            926..1981
                     /locus_tag="VMU21_05595"
     CDS             926..1981
                     /locus_tag="VMU21_05595"
                     /inference="COORDINATES: protein motif:HMM:NF044557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77035.1"
                     /translation="MDTKHLMQQIKLNCTISDAHFWGYYSICGLLLRLRELYRNERSL
                     TPWDEIPRGDITDWIASTEAHWKALEHHPLRPLEIGDSLYDPFEVDGLNDLLSAQGLV
                     YGGGYGRFNKPTFFLAHRLEIAERGDYRIYYAGRELCRDLSTSVAMLQGRCIFMRLDS
                     LKMLLWDKLQELHSRKFGGVLREAFASHGIEGPSLSPEALSSKIDSLASRLSDLFILH
                     ELGEAFEDEYADEWLSILSHNQDRATEFYVRGIKDLLADTSEKGPLKWITDSMDIPLL
                     NFYMVFMDGIRKEIFPGMVTAFQTFVENREWSLIEHARMNGYKKATELRGHVLRLWRE
                     GKGDTLGTSIREILSDK"
     gene            2196..3386
                     /locus_tag="VMU21_05600"
     CDS             2196..3386
                     /locus_tag="VMU21_05600"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HUO77036.1"
                     /translation="MSVKELTNLKVTDLWKEYNGLGDFWDMQEEAVKDFRRRFIERAL
                     EAERDLLIGCRYYERASERKDYRNGYWRRWITVKDGRLEIRMPRMRGLHYESGIIPRY
                     KHRVDEVDSALLKVFLYGASTRLAGEALRPLLGEGVSAQTISNIAGSLDEDLKRYHRR
                     RVQDLYLYLFLDGIILKTRTGFGAKKKAVLVAYGIKVDGRRELIDFTVTNAESEKRWW
                     RFLNDLARRGLRGEALGLVVTDGNPGLENAVDLVWPFVKRQRCWAHKLRNVANYLKRK
                     HVDRCMKEAAAIYKATNRKEAEQAYGKWQKKWTPLAPKAVKCLEKDLEELLNFYSCPK
                     EIWVKVRTTNVIERAFREVRRRTRPMSCFNNVQSIERIVYAKLTHLNEQWGSKPLKEF
                     THND"
     gene            3580..4671
                     /locus_tag="VMU21_05605"
     CDS             3580..4671
                     /locus_tag="VMU21_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO77037.1"
                     /translation="MTKDKKLNELKTLLTVSSLINSTLDPSTVREKTIEAITTLLDAE
                     AGSLLLLDEEKGGLFFDVATGEKGARLRHFRLANGEGIAGWVAEHKQHLIVNDVRSDP
                     RFSKRADEKSGFTTKSILCVPLCSKGRVIGVIEGINKKKGTFLEEDIRVMTALADQVA
                     IAVENAELYTELKEALYSMAEALAGAIELRDSQTGIHIRRVMDYSLIIGRYLGLNQEE
                     LETLKLSAALHDIGKLGVRDDILLKQGYLNEEELRAMKCHTDYGSEILTHIKHLNKVI
                     PVVRGHHEHYDGKGYPDGLKDSDIPIEARIIAVADAFDAMITDRPYRKKLSMQGAFEE
                     LKRCSGTQFAPEVVNALFTAWNNGELNFS"
     gene            4790..6124
                     /gene="hisD"
                     /locus_tag="VMU21_05610"
     CDS             4790..6124
                     /gene="hisD"
                     /locus_tag="VMU21_05610"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HUO77038.1"
                     /translation="MQILKTSHEIETFLSLLRERASGGSPRVEKTVKRILSDVKRDGD
                     SAVIHYTEKFDSLKVKNLRITPAEVTRHANKADRKIVRSLERSAKRICSFHRMQREES
                     WVIWDTEYKKIPLGKGLQRKKVSRGALLGQMLTPLKRVGVYVPGGKASYPSTVLMNVI
                     PAQVAGVGEVALSVPTPRDEVNPYVMAAVEMLEVKEVYRIGGAQAVGAMAYGTRTIPR
                     VDKIVGPGNIFVAMAKKLVFGEVDIDMIAGPSEVLIIADKTANPRFIAADLLSQAEHD
                     ELASSTLITDSGPLAVAVQKELKSQLADLKRKAIAESSIKNYGAIIVTTDMEKAVKLS
                     NEIAPEHLEVMTRNPFALLSGIRNAGAIFLGPWTPEPLGDYAAGPNHTLPTGGTARFF
                     SPLGVYDFVKRSSLLSFSREGFLGLAKTVMTVSEIEGLNAHGNTIRVRLEKS"
     gene            6111..7193
                     /gene="hisC"
                     /locus_tag="VMU21_05615"
     CDS             6111..7193
                     /gene="hisC"
                     /locus_tag="VMU21_05615"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HUO77039.1"
                     /translation="MKKANITALVRPNIRTLRAYEAKEIPSKAKLDANESPYPLADCL
                     SHKFVIPLGRVMTNRYPDPEAKELKKVVGTYFGVPSGNLLQGNGSDELISSLITTFGG
                     PVLYPVPTFSMYGIISQALGQKGIAVPLDRSFDIDLERVLKAMHNLNPKLIFLSSPNN
                     PTGNCFSPGKTLKIIEKSKGIVVVDEAYQPFSSQKGFLPFLKDYQNLVIMRTLSKIGL
                     AALRLGFLIAHTEIIREVNKVRLPFNVNSISQALAIAALKDRRRLRSSIKTIIRERER
                     LQEEMGKIRGVTTFPSEANFILFKVKDPDSVCSSLLKKGVLVRNIHSGIKGCLRVTAG
                     TPEENTLFLKALKSASTEGSPPLVQG"
     gene            7693..8163
                     /locus_tag="VMU21_05620"
     CDS             7693..8163
                     /locus_tag="VMU21_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HUO77040.1"
                     /translation="MSAVMQFLYNLVLALWVGGISIFTFIVTPLIFRSFGRDMAGEVV
                     GKLFPGYFLYTLLLSVVALILFLASRPSLGKSAHTWSLPLIVLAIIINLFVSLKLHPE
                     IKKIKQEIPSFEAAQESPLRKRFGQLHGVSATLNLILLADGVVLFVISTVLKKQ"
BASE COUNT         2191 a         2009 c         2202 g         2042 t
ORIGIN      
        1 tagtgatgct tccttttgcg tttgtggcag cgcttggcac cgaggtgggc ggcagggtga
       61 tgagccttgc gggaaggagc gcggcatttg gatggaggcc gatggagtcg tatctgaccg
      121 gcaagaagac aaagaaggaa gacaagaaac aggaacagaa gaaataactg ccttcaccct
      181 ggcagttccc gggaaccctt cacactgcaa gtaccgggag ctgccggagt acaaaaaata
      241 tcatcccccc tatcgctcaa cccccgaaga gccagcgctt cagattccag aaatgaatga
      301 ccttttgtgt gaacgcagtc tgatccacgt ttgtggccag gtcccttaac tccattctcg
      361 tcttttctat ctccagatcc tgtgccctct ccaggccacc atccgcaata aaatggcgaa
      421 tcttttccat ctctcctgca tcaagccaga cgccgtgctt cccacattca tctatgatca
      481 cccctgatat ttttccgaaa ttcttccggt tcatcaattt tccgcaccga acgcacgaaa
      541 tatattccac agggtctttc aggggttcac ggagatattc atccttaaaa ttgtctctgt
      601 acacatcgta ttctctggta aggagatgga attctcccct gtcgagccag agtcccccgc
      661 agcgcgggca gaggtcatat tcttcacccc ccgcattgtg aacatcgagg ggttgtttgc
      721 acctggggca cgcaggaggg cgggcttcca ctttgtccct gagatgacca ccacacaaag
      781 gacagatggt gtacgcggca ttgatatcct ttccgcagaa gtcacatttc ctggtctcgt
      841 tcatccccac tatcttattt ccaggaaact cccctgtcaa ggttttcctt ttgtcatttg
      901 gaccgttttg tcttaagata caggtatgga cacgaaacat ctcatgcagc agataaaatt
      961 gaactgcacc atctctgatg cccatttctg gggatattac tcaatctgtg gcttgctatt
     1021 aaggctgagg gaactgtaca gaaatgaacg ctctctcacg ccatgggatg agataccaag
     1081 gggtgatatt acagactgga tcgcttccac cgaggcacat tggaaggccc tggaacacca
     1141 cccgctccga cctctggaga tcggggacag tctctacgat ccctttgaag tggatggact
     1201 aaacgatctc ctttcagctc aagggctcgt ctatggaggc ggctacggga ggtttaacaa
     1261 gcccactttt ttcctcgcac accgccttga gatcgcagaa cgtggggatt accggatata
     1321 ttatgcggga agggaactct gcagagacct ctccacgtcc gtggccatgc tccagggaag
     1381 atgtatattc atgagactcg actcacttaa gatgctcctc tgggataagc tccaggaatt
     1441 acattcgagg aagttcgggg gcgtgctgag ggaggccttc gcatcacatg ggattgaggg
     1501 cccctctctt tctccggaag ccctctcttc caaaatcgac tccctcgctt cccgtctctc
     1561 ggatctcttc atactccacg aactgggtga ggcattcgaa gatgaatatg cggacgagtg
     1621 gctcagtatt ctgagccata atcaggacag agcgacagag ttctatgtga gggggataaa
     1681 ggaccttttg gcagatacct cggaaaaggg acccctcaag tggattactg attccatgga
     1741 tatacctttg ctcaattttt acatggtctt tatggacggg atcagaaagg agatattccc
     1801 aggaatggtc actgcctttc agacttttgt ggaaaacagg gagtggtcct taatcgagca
     1861 cgcgaggatg aatggctata aaaaggcaac agagctcagg gggcatgtcc ttaggttgtg
     1921 gagagaggga aagggagata cgttaggaac ctccataaga gaaatcctgt cagacaagtg
     1981 acatgtgcca tgggggaagc gcactgggta aatatatagt ttcgtcgtag atggcagatg
     2041 tcacagcttt ggcttgcggg gagtgcccgc aggccttaac agttcatcgc tcccgtgtgg
     2101 tagcgtaaaa tcttgtgtgt aaaaacttga agtaaaaaaa gagcgggtgt attctccatt
     2161 gttctgtcca aggaacaaga aaggaggata cacccatgtc agtaaaggaa ctgacgaatt
     2221 taaaggtaac agatttatgg aaggaataca acggtcttgg agatttctgg gatatgcagg
     2281 aggaggcggt aaaggatttc aggaggcggt tcatcgagag ggcccttgaa gcggagcgag
     2341 atttgctgat agggtgcagg tactatgagc gagcatcgga gcggaaggac tatcgtaacg
     2401 ggtactggag gagatggatc acagtaaagg atgggcggct tgagatacgg atgccgagga
     2461 tgagaggact tcactatgag agcggcatca tcccgcggta caaacatcgg gtggatgaag
     2521 tagactcggc gttgctgaag gtgtttcttt acggggccag tacgagactt gcgggggagg
     2581 cgctgaggcc gctcttgggc gaaggggtga gtgcccagac gatctccaat attgcgggga
     2641 gcctggatga ggatctgaag agataccacc gccgcagagt ccaggaccta tatctctatc
     2701 tttttttaga cggcataatc ctgaagacca ggacggggtt tggggcgaaa aagaaggcgg
     2761 tgctggtggc ttacgggatc aaggtggatg gcaggaggga gctcatcgac tttacggtca
     2821 ccaatgccga gagtgagaag agatggtgga ggtttctcaa tgaccttgcc cgcagagggc
     2881 tgaggggcga ggcgttgggg ttagtggtga ctgacggcaa cccggggctg gagaacgccg
     2941 tggaccttgt ctggcctttt gtgaagaggc agcggtgctg ggcgcacaaa ctcaggaatg
     3001 tggccaatta tcttaagagg aagcatgtgg atcggtgcat gaaggaggcg gctgcgattt
     3061 ataaggccac caataggaaa gaggccgagc aggcctacgg caaatggcag aagaaatgga
     3121 ctcctctcgc cccaaaggct gtcaaatgcc tggagaagga cctggaggag ttgcttaatt
     3181 tctacagttg ccccaaggaa atatgggtta aagtgcgaac caccaacgtc attgagaggg
     3241 ccttcaggga ggtgaggaga aggacgaggc ccatgagctg tttcaacaac gtccagagca
     3301 tcgagagaat tgtttacgca aagttaactc acttgaacga gcaatgggga agcaagccct
     3361 taaaggaatt tacacataac gattgacatt acctcccgtg tcaatttgct tgcataagta
     3421 ggtttcaaga gatacaatgt ctttgaatca tgaaagtcgt ttcatctaag gcagagagga
     3481 atccgagatc ttcattgatt aagccacgcc tcatcacaat catcgtcttt tctctcaccg
     3541 catccctgct ggccccacag gaatgggaag aataacgcga tgacgaaaga taaaaaactg
     3601 aacgagttaa aaactctcct caccgtttct tctctcatca actctactct cgatccctca
     3661 acggtccgtg aaaaaacaat tgaggccatt acgactcttc tcgatgcgga agcaggcagc
     3721 cttctgcttc ttgatgaaga aaaaggtggc ctgttctttg atgtggcaac aggtgagaag
     3781 ggagcccgat tgagacattt tagactcgcg aacggagaag ggatcgcggg atgggttgca
     3841 gagcataaac aacatctcat cgtgaatgat gttcgctccg atccccgttt ctctaaaaga
     3901 gcggatgaga aaagcgggtt taccacaaaa agtatattat gcgtgcctct gtgttcgaag
     3961 ggcagagtca tcggcgttat cgaggggatc aacaaaaaaa aggggacctt cctggaagag
     4021 gatatcagag tcatgacagc cctcgcagat caggtagcga ttgccgtaga aaatgcagaa
     4081 ctctatactg agttgaaaga ggcactttac agcatggcgg aagccctggc aggcgccatt
     4141 gaactgagag attcgcagac cggaattcat atacggcggg taatggatta cagcctcatt
     4201 ataggaagat acttggggct gaatcaggag gaactcgaaa cacttaagtt atctgccgcc
     4261 cttcacgata tcggaaaact cggagtaaga gatgacatat tacttaaaca gggatatctc
     4321 aatgaggagg aattacgggc aatgaaatgc catactgatt atggatctga gattctcacc
     4381 cacataaagc atctcaataa ggtgatacct gtggtgaggg gtcaccatga gcactacgac
     4441 ggcaagggat atcctgatgg cctgaaagat agtgatattc ctatcgaagc caggatcatt
     4501 gcagttgcag atgccttcga tgccatgatc accgatcgac cttacagaaa aaagctcagc
     4561 atgcagggag cgtttgaaga actcaaacga tgttcgggca ctcagttcgc acctgaggtt
     4621 gtgaacgcac ttttcaccgc atggaataac ggtgaattga atttctcatg agcattgtac
     4681 tatgcccgtg ggtctattca ttgtaactcc acggtagccg tgtcttggtt ttctgccatt
     4741 gtcttaccac ccttgcatat tgccctgtga aatattagaa tcatacatca tgcagatact
     4801 gaaaacatct catgagattg agacctttct ctctctcctc agggagcgtg catccggcgg
     4861 ctcccccaga gttgaaaaga cagtgaaaag gatactctct gacgtgaaga gagacggcga
     4921 tagtgccgtg attcattaca cagagaaatt tgactccctc aaagtcaaaa acctcaggat
     4981 aacgccagca gaggtcacac gccatgcaaa taaggcggac aggaagatcg tgagatccct
     5041 tgagcgttct gcaaaaagga tttgttcttt tcacaggatg cagagagaag aatcatgggt
     5101 tatctgggat acagaatata agaagattcc gctcgggaaa ggcctgcaga gaaagaaggt
     5161 ttcccgcggc gctcttctgg gccagatgct gactccactc aagagggtcg gtgtctatgt
     5221 cccaggagga aaggcatcct atccttccac cgtgcttatg aatgtgatcc cggcccaggt
     5281 ggcaggagta ggggaggtcg cccttagtgt gcctactccg cgcgatgaag tgaatcccta
     5341 tgtcatggca gctgtcgaga tgttggaagt gaaggaggtg taccgcattg gcggtgccca
     5401 ggcagtgggg gccatggcgt atggcaccag gaccatcccg agggttgaca agatcgtggg
     5461 accagggaat atttttgtgg ccatggcaaa gaagcttgtg ttcggagagg tggatattga
     5521 catgatcgca ggccccagcg aggtgctcat cattgctgac aaaacagcca acccccgctt
     5581 tattgccgca gatcttctga gtcaggcaga gcatgatgag cttgcctctt ccaccctcat
     5641 taccgattca gggccacttg ctgtggcagt gcagaaagag ctcaaaagcc agcttgcaga
     5701 cttaaagaga aaggcgatcg cagagagctc cataaagaat tacggagcga tcatagtgac
     5761 aacagacatg gaaaaggcag ttaagctctc caatgagatc gcgcccgagc atctggaggt
     5821 catgactcga aacccctttg cccttctttc cggcataagg aatgcgggcg ccatttttct
     5881 gggaccatgg acccctgagc cccttggaga ttacgcagca ggaccgaacc atacccttcc
     5941 cacaggaggt accgcacgct ttttctcacc cctgggtgtc tatgactttg taaaacgctc
     6001 cagcctcctc agtttctcga gggagggttt tctgggactt gcaaagacgg tgatgaccgt
     6061 atccgagatc gagggattaa atgcccatgg caataccatc agggtgagac ttgaaaaaag
     6121 ctaacatcac cgcattggtg agaccgaaca tccgcactct ccgtgcctat gaagcaaagg
     6181 agataccctc caaggcaaag ctggatgcca atgaaagccc ctatcctttg gcagactgtc
     6241 tttcccataa atttgtaatt ccgttgggac gggtgatgac caaccgttac ccggatcctg
     6301 aggcaaagga gctgaagaag gtggtgggga cttatttcgg ggtcccgtca gggaatctcc
     6361 tccagggaaa cggttccgac gaactaatct cttccctcat caccaccttc ggtggccctg
     6421 tgctgtatcc ggttcccacc ttctccatgt acgggattat ctctcaggcc ctgggtcaaa
     6481 aggggatcgc ggtcccactg gacagatctt ttgacatcga tttggaaagg gtcttgaagg
     6541 caatgcataa ccttaacccg aaactcatct tcctcagctc gcccaacaac cccacaggga
     6601 attgtttttc tcctgggaag actttgaaga tcatcgagaa gtcaaagggc attgtggtag
     6661 tggacgaggc ataccagccc ttttccagcc agaagggctt cctcccattt ctcaaagact
     6721 atcagaacct tgtgatcatg aggacattga gcaagattgg ccttgcagca ctccgtctcg
     6781 gctttctcat cgctcacaca gagattatac gagaagtgaa taaggtgaga ttgcccttca
     6841 atgtcaattc catttcccag gctcttgcca tagctgcact gaaagataga aggaggctgc
     6901 gttcttccat aaagacaatc atcagagaaa gggagcggct tcaggaagag atgggaaaaa
     6961 taaggggcgt taccaccttt ccttcagagg cgaatttcat cctgttcaag gtgaaggacc
     7021 ctgacagcgt gtgttcttca ctgctgaaaa agggtgtcct tgtgaggaac atacatagtg
     7081 gaataaaggg gtgcctcagg gtgactgcgg gtacgcctga agaaaatacc ctctttctca
     7141 aggcattgaa aagtgcatcc acagaaggtt cccctccact ggtgcaaggg taataagaac
     7201 cgcctcatca ggggcacatg aaaagaagaa ctcttttgtc cctcgtcctc ttcttcttat
     7261 gtcattaacc cttgaagccg ccaggaacaa gggtgagcct gtccacgtca agaaagggga
     7321 tcgcctcaca ttcacaaacg ggatgattca ggtgagggag aaggcaggag agagagggac
     7381 atggatcgtg gtgaacatct cgggaattcc tgtcctctca acaccagggt gagtccggtc
     7441 catgccctct ttgtacatga taaaattcct tatttcttct tcttttttgc tgcggtcttt
     7501 ttagtgccgc agggagaaga cttgctgccc gacttacacg ccatttttcc ccctcctttc
     7561 ccaaaagata ttctattttc actataccaa tccttcgaag tgctgtcaaa caaaaatcgt
     7621 ccgtttataa cagctcaaaa aaatgaattg gctgttgccc ttttttcctt tatactatac
     7681 cgagcgatga ccatgagcgc agtgatgcag ttcctctata atctcgtcct tgccctctgg
     7741 gtgggaggga tttccatttt taccttcatc gtcacccctc tgatattcag atccttcgga
     7801 agggatatgg cgggtgaggt ggttgggaag ctcttccccg gctattttct ttacaccctc
     7861 ctcctttcag tagtggcact catccttttt ctcgcatcac gaccctccct cgggaaatca
     7921 gcacatacct ggtccctgcc tctcattgta ttagccatca tcataaacct ttttgtatcc
     7981 cttaagctcc atcccgagat aaaaaagata aagcaggaga tcccttcctt tgaagctgct
     8041 caagagtcac ccctcaggaa gagattcggg cagctccatg gggtgagtgc cacccttaac
     8101 cttattctcc ttgccgatgg tgtcgtcctc ttcgtcatca gtactgtttt gaaaaaacaa
     8161 taggacaatc aaggagacac ttttttaccc attagtaatg aacagtcttg agcgcttttg
     8221 cgtgattcag tggcgagtta tcagctatga gcttcttttg cttctgcgtt tttgttcttc
     8281 agtacttttg gtataatgag tggctaggga aactcaggaa gaaggctcat gagcttccac
     8341 tgcttgatac cgttctcggt cacataggcc catttctggt tatcgatgag actcctgagt
     8401 ctgtttgcag taagggtgta gtactcgatc tccacctgta ccag
//
LOCUS       DATKOU010000122         7345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4005703, whole genome shotgun sequence.
ACCESSION   DATKOU010000122 DATKOU010000000
VERSION     DATKOU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4005703"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VMU21_05625"
     CDS             <1..697
                     /locus_tag="VMU21_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HUO77041.1"
                     /translation="EGYIDSDTYISGRSIDAALCAAGAVMEAAKQCREGKVSRAFCAV
                     RPPGHHAEADRAMGFCIFNNVAVGTRFAQKIGFEKVFIIDFDVHHGNGTQHIFEEDES
                     VYYFSTHQYPHYPGTGSDSEHGTGKGKGFTRNIPMMSGSGDREFFSAYHDLLPRMVKE
                     FDPGILMVSAGYDIHRDDPLASIRVSDEGIRDMVKSILSLRLPTVFSLEGGYNLKALG
                     GSVLITIEEMMKD"
     gene            complement(951..2864)
                     /gene="glgB"
                     /locus_tag="VMU21_05630"
     CDS             complement(951..2864)
                     /gene="glgB"
                     /locus_tag="VMU21_05630"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HUO77042.1"
                     /translation="MGPEKRMRVLTEFDLHLIGEGSHYKKYEKLGSHIREVEGMKGVH
                     FAVWAPNAEAVSVIGDFNGWDSGPHPMQSLGDSGIWEIFIPGLGEGTLYKFEIRSRFS
                     GYVTQKADPFGFYFEVRPKSASIVYDIEGFGWNDSHWMAKRAERNWLASPVALYEVHL
                     GSWMRTAEGSDGWLSYKEIAERLIPYVTGLGYTHIQLLPITEHPLDASWGYQTLGYFA
                     PTSRFGTPKDFMHFIDLCHQKGIGVVLDWVPAHFPTDGHGLGFFDGTCLYEHADPKKG
                     FHPDWGTKIFNYGRNEVRNFLISNAMFWFEKYHLDGLRVDAVASMLYLDYSRREGEWI
                     PNMYGGRENLEAIDFLKKFNEVSHQYHPGILTIAEESTAWPNVSRPTYIGGLGFSMKW
                     NMGWMNDTLEYFSKDPVHRKYHHNNLTFSLLYAFTENFVLPLSHDEVVHGKGSLLNKM
                     PGDMWQKFANLRMLYGFMYGHPGKKLLFMGGEFGQWDEWNVDKSLDWHLLEHEPHKGL
                     QRFVRDLNGIYRAEPSLYEIDFDHKGFEWIDFHDVEGSIVSFLRRAKSHDDFTVFVYN
                     FTPVPRQNYRLGVPKSGFYREVLNSDSSLYGGSNLGNAGGVYAENAPFQHRPYSITFT
                     LPPLSVLMFKPAQ"
     gene            complement(2854..3303)
                     /locus_tag="VMU21_05635"
     CDS             complement(2854..3303)
                     /locus_tag="VMU21_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77043.1"
                     /translation="MDQKLNELKKKVFHEILDIVGRVFIVARYSEHVIIGKRGFTEEE
                     KKDGLVLVFNVHMNFVWNDSGISSTLVFGTAPQKCFVPAEDIVAIYSPELQCQFISAP
                     QTSHEMPPGKDEEEKAGPNVVKVDFRKKKAPRGSGTTRQGGGDTHGS"
     gene            3580..3780
                     /locus_tag="VMU21_05640"
     CDS             3580..3780
                     /locus_tag="VMU21_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HUO77044.1"
                     /translation="MEKGTVKWFNESKGFGFITREGGGDVFVHYSAIQDNGFKSLAEG
                     QAVSFEIVDGPKGPKAVNVTKN"
     gene            3922..4443
                     /locus_tag="VMU21_05645"
     CDS             3922..4443
                     /locus_tag="VMU21_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUO77045.1"
                     /translation="MEDSEEQVEIVNEKGEVIGLAPRAEVHSNPSLMHRVVHVLVFDP
                     GGRLLLQKRSLTKDVAPGKWDTSVGGHVGIGEAMALSAAREMEEELGISDCDMEYLYS
                     YVHRGRNETELVCTYRCIYSGDIPFNKNEIDEVRFWSLEEIKSAMGKEILSAHFEDEF
                     KTYLGHTPRTSGP"
     gene            complement(4416..5051)
                     /locus_tag="VMU21_05650"
     CDS             complement(4416..5051)
                     /locus_tag="VMU21_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO77046.1"
                     /translation="MKVCEIFASIQGESSYAGVPCTFVRMSGCNLKCSYCDTVYAYEE
                     GTELTEDEITGKVRAIGLRTVEITGGEPLLQPGVLSLTKRLLDHEFRVLIETNGSQDI
                     RQVDQRAVLILDVKTPGSGMANTFLMSNLDLLRPVDELKFVITGRKDYEWSRYFVKEH
                     SLTGKCTLLFSPAFGMLHPKDLSQWIMEERLEVRLNLQLHKYIYGPDVRGV"
     gene            complement(5251..5631)
                     /locus_tag="VMU21_05655"
     CDS             complement(5251..5631)
                     /locus_tag="VMU21_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77047.1"
                     /translation="MKRTLLIALSVMLTVALVTFAIAAGKSKMELKAGDEIYACNCGE
                     SCNCDTMSRNAGKCTCGKEMVKAKVTKIEDGKAQLKGEGWAKERTFKTNGKYVCGCGP
                     ECKCDTISQNPGKCTCGKEMKKAS"
     gene            complement(5753..6196)
                     /locus_tag="VMU21_05660"
     CDS             complement(5753..6196)
                     /locus_tag="VMU21_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77048.1"
                     /translation="MVQITMTKESLKRTKGFLSMWSFRKKFGDSRKVRMASRLYGKAV
                     RLDEKGKPYEAIVMVCRALQAVRKSDAGLTEPDALSQVMTATTLFDKLATKVGRPEIV
                     LTALREALNLCDRAMAADSRFIELVQPYRDRFHHRIGLIAAQPEQ"
     gene            6601..6882
                     /locus_tag="VMU21_05665"
     CDS             6601..6882
                     /locus_tag="VMU21_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO77049.1"
                     /translation="MIKSFKNKETENVYQLEGSGKLPGDIQQVALRKLRMINNAINLL
                     DLRIPPANKLEKLKGDRRGQHSIRINDQWRICFTWRDGDAYEVGITDYH"
     gene            6893..7195
                     /locus_tag="VMU21_05670"
     CDS             6893..7195
                     /locus_tag="VMU21_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HUO77050.1"
                     /translation="MKKKLYPVHPGEILSEEFLKPMGLSQNRLALNIGVSARRINEIV
                     LGKRGITADTALRLARFFGTSAQFWTGLQSQYDLDVTAEALRGRLEREVKAYAKAL"
BASE COUNT         1797 a         1821 c         1827 g         1900 t
ORIGIN      
        1 tgaggggtat atagactctg acacatacat ttcggggaga agtattgatg cggcattgtg
       61 cgcggcagga gcggtcatgg aggcagcgaa gcaatgcagg gagggcaagg tctcgagggc
      121 cttctgcgca gtgaggccgc ccgggcatca cgcagaggct gaccgtgcaa tggggttttg
      181 tatcttcaac aatgtggcgg tgggaacacg gtttgcgcag aagatcggct tcgaaaaggt
      241 ctttatcatc gattttgatg tgcaccacgg aaacggtacc cagcatatct ttgaggaaga
      301 cgagagcgtc tactacttca gcactcacca gtatccccat tacccaggga cagggagtga
      361 ttccgagcac ggcacgggaa agggcaaggg ctttactcgt aacattccca tgatgagcgg
      421 ctcaggggac agggaattct tctccgcgta tcatgacctc cttccccgga tggtgaagga
      481 gtttgatccc ggcattctca tggtgtctgc cggatatgac atccatagag atgacccatt
      541 ggcatccatc agggtttctg atgagggaat cagggatatg gtgaaatcca tactctccct
      601 tcgtctcccc acggtatttt ccctggaagg gggatataac ctgaaagccc tgggagggtc
      661 ggtcctcatc accattgaag agatgatgaa ggattaatcc accgcatcta tgagctcctt
      721 gttccgagag agcatccgtc tcttgacaag acaatggccc ttcccgattg taccagggtc
      781 tacatacctc ccaacactct gcacttatgt aatgtcaatg tctgctcttg cggacggcac
      841 ataacccttc cgcctcaggc ggattgccat tggagccgga cacctctgag gcatttatga
      901 attatcaagg tttaaagaag gagtcaggtg ggaaccgcgg cgttcctgtg ctattgtgcg
      961 ggcttgaaca tcaacactga cagcggcggc agggtgaagg taatggaata cggccggtgc
     1021 tggaaaggag cgttctccgc atatacgccc cctgcatttc ccagattgct tcccccgtaa
     1081 agggaagagt cgctgttcag aacttctctg taaaaccctg acttgggaac ccccagccgg
     1141 taattttgcc tcggcacagg ggtgaagttg tatacaaaaa ccgtgaagtc atcatggctc
     1201 tttgcccttc tgaggaacga gacgatgctg ccttccacat cgtggaaatc gatccactca
     1261 aatcccttat ggtcaaaatc gatctcatag agggacggct cggccctgta aattccgtta
     1321 aggtccctga caaagcgctg gagccccttg tgcggctcgt gctccaggag atgccagtcg
     1381 aggctcttgt caacattcca ttcatcccac tgcccaaact ctccgcccat gaagaggagc
     1441 ttcttcccgg gatgaccgta catgaacccg tagagcatcc tcagattcgc aaacttctgc
     1501 cacatgtccc caggcatctt gttcaataat gatccctttc cgtgcaccac ctcgtcatgg
     1561 gaaaggggaa ggacaaaatt ctctgtaaag gcgtagagga ggctgaaggt gagattgttg
     1621 tggtgatact tcctgtgcac agggtccttg gagaagtact ccagggtatc gttcatccag
     1681 cccatgttcc atttcatgct gaaccccagc ccgccgatgt aggtgggcct ggagacattg
     1741 ggccatgcgg tggactcttc ggcaatggtg agtatcccgg gatggtactg gtgggatacc
     1801 tcattaaact ttttgagaaa atcgatagcc tccaggtttt ctcggccgcc gtacatgttg
     1861 ggaatccact ccccttccct tctcgagtaa tcgaggtaga gcatagacgc aaccgcgtct
     1921 acgcggagac cgtcgagatg gtatttttcg aaccagaaca tggcattgga tatcaggaag
     1981 tttctcacct cgttcctgcc ataattgaat atctttgtgc cccaatcagg gtggaaccct
     2041 ttcttcggat ccgcgtgctc atagaggcat gtgccatcaa agaatcccag accatgccca
     2101 tcggtgggga aatgcgcggg cacccagtcc aggactaccc caatgccttt ctggtggcaa
     2161 aggtctataa aatgcatgaa atccttggga gtgccgaacc tgcttgtggg cgcaaaatag
     2221 ccaagggtct gataccccca tgatgcatca agagggtgtt ctgtgatggg aagaagttgg
     2281 atatgcgtat agcccaaccc tgtgacatag ggtatcagcc gctctgcaat ctccttgtag
     2341 gaaagccacc catcacttcc ttcagctgtc ctcatccatg agccgagatg gacctcgtat
     2401 agtgccacag gggatgccag ccagttcctc tccgccctct ttgccatcca atggctgtcg
     2461 ttccatccga acccctcgat gtcatacaca atggaggcac ttttgggcct cacttcaaaa
     2521 taaaaaccga aggggtcggc cttttgggtg acatagccgc tgaatcttga ccgtatctca
     2581 aacttgtaca gagttccctc acccaatcct gggatgaaga tctcccagat tccggaatca
     2641 cccagacttt gcatcggatg ggggccgctg tcccagccgt tgaaatcgcc gatgacgctc
     2701 acagcctctg cattgggtgc ccataccgca aaatgaacac ccttcatccc ttcaacttcc
     2761 cttatatggg agccgagctt ctcatatttc ttgtaatggc tgccctctcc tataagatgg
     2821 aggtcaaatt cagtgagcac ccgcatcctt ttctcaggac ccatgggtat ctcctccccc
     2881 ttggcgggtc gttccggagc ctctgggcgc tttcttcttc ctgaaatcca ctttcaccac
     2941 attggggcca gccttttcct cttcatcctt cccgggcggc atttcatgtg aagtctgcgg
     3001 tgctgaaatg aactggcatt gcagttcagg agaatagatg gcaacgatgt cttctgcggg
     3061 gacgaaacat ttttgggggg ctgtgccgaa tacaagggtt gaggaaatgc cggagtcatt
     3121 ccacacgaag ttcatgtgca cattaaagac caggacaagg ccgtcctttt tttcttcctc
     3181 ggtgaagccg cgttttccga tgatcacatg ttctgaatat ctcgccacaa tgaagacacg
     3241 gcccactata tcgaggattt cgtggaatac cttcttcttc agttcattca gcttctggtc
     3301 catgagcgaa ttgtagcaga ttccagaggt tttgacatca gcacactaca aaaaaccaac
     3361 caaaaaaaac gaataaaata aacaaaacat cttgaccttt tttgaaattt atgtaaaatt
     3421 atcaatgaag tttcggaagt ttttgtcgta tgtcgttgac agggccgcaa agactttgga
     3481 ctttgcggtt tttttgtggc acaatttccg taatcttcac tacttttatc tcgaaaaagt
     3541 tttttcgagg aaacatcttt agcaaggagg tatcgcagga tggagaaggg aactgtgaaa
     3601 tggttcaatg agtccaaggg ctttggattc atcaccaggg aaggcggcgg agatgtgttt
     3661 gtccattatt ccgctattca ggacaacggt tttaagtccc tggctgaagg ccaggcagtg
     3721 agctttgaga tcgttgatgg ccccaaaggc ccgaaagcgg taaacgtaac aaaaaattaa
     3781 gccaataaca atgcaggcct tcccgggcct gcatctctca cgtccccctc agtgttcaca
     3841 gaagatgggt gcattgataa aatactagag gttcccattg tggatgctca attcagcatg
     3901 aacggatgat cgggtaatga catggaggat tctgaagagc aggtagagat cgtgaatgaa
     3961 aagggcgaag tcattggcct agctccacgc gctgaagttc attctaatcc ttccctgatg
     4021 catagggtcg tgcatgtcct tgtattcgat cccggaggca gactgctcct ccagaaacgc
     4081 tccctcacca aggatgtggc tccaggcaag tgggacacct cagtgggagg ccatgtgggg
     4141 ataggtgagg caatggccct ctctgccgca agagaaatgg aggaggaact gggcatttct
     4201 gactgtgata tggagtatct ctactcatat gttcaccggg gccggaacga aacagagctt
     4261 gtgtgcacct accgctgcat ttacagcgga gatatccctt tcaacaaaaa cgagattgat
     4321 gaggtgaggt tctggagcct tgaagagata aagagcgcca tgggcaaaga gatactaagc
     4381 gcgcattttg aggacgaatt caagacatat cttggtcata ccccgcgcac atcaggtcca
     4441 tagatatatt tgtgcagctg gagattcagt ctcacctcaa gcctctcttc cataatccat
     4501 tgcgagaggt cctttggatg gagcatcccg aaggcagggg aaaagagtag ggtgcatttt
     4561 ccggtaagag aatgttcctt tacaaaatat cttgaccatt cataatcttt ccttccggtg
     4621 atgacgaatt tgagttcatc cacaggtcta agcagatcga gattggacat gaggaatgta
     4681 tttgccatac cgctccccgg ggtctttaca tcaaggatga ggaccgcccg ttggtccacc
     4741 tgccgtatgt cctggcttcc gttcgtctct atgagtaccc tgaattcatg gtcaaggagc
     4801 ctctttgtca gggaaaggac acctggctgg agcaacggtt ctcctcctgt aatctccact
     4861 gtcctcaggc ctatggcacg caccttccct gttatttcat cctctgtgag ttctgtgcct
     4921 tcctcatacg catacaccgt atcgcaatag gaacatttca ggttgcagcc tgacatgcgt
     4981 acaaatgtgc agggcacgcc cgcataactg gattccccct gtatgctcgc gaatatctcg
     5041 cagaccttca tgggctaaat aagatatctg ggtgttgaaa gatagcgtcc atggtgaaag
     5101 gtatcatgtt tgggtctttg atgtcaaaag agggtgatgg tgaaaatagt gaacgcttgg
     5161 ccactttaca aaaaatcaag gaagtaccgg tgaggggaga aaaaaggcgg gattgctccc
     5221 gccttttttt catgctcgga gaataggaac ttagcttgct ttcttcattt ctttgccgca
     5281 ggtgcatttg ccagggttct ggctgatggt gtcgcatttg cactctgggc cacatccgca
     5341 cacatacttg ccattggtct taaaggtcct ttcttttgcc catccttcgc ccttcagctg
     5401 ggccttgcca tcctcaatct ttgtcacttt tgccttgacc atttctttgc cgcaggtaca
     5461 cttgccggca ttccgcgaca tggtgtcgca gttgcatgac tcaccgcagt tgcatgcata
     5521 aatctcatct cctgctttga gttccatctt gctctttccg gcggcgatag cgaatgtgac
     5581 gagcgcgaca gtgagcatta ctgacaatgc tatcaagaga gtccttttca tcctttttcc
     5641 tccttaaata taaagatagt ttatcaaata taatctaaat ttagaaattt gtaaactttc
     5701 tctaagcagc cctgcaacgt ttcttgtatc atcatgctcg tccctctaat gattactgtt
     5761 ccggctgcgc agcaattaaa cctatgcgat gatgaaacct gtcgcgataa ggctgtacaa
     5821 gctctataaa tcttgaatca gctgccatag cccgatcgca gaggttaagg gcctctctga
     5881 gggcagtgag cacaatctcc gggcgtccca ctttggtcgc cagcttatca aagagagtgg
     5941 tagcggtcat aacctgactg agtgcatccg gttctgtcaa ccctgcgtct gattttcgta
     6001 ctgcctgaag tgcacgacat accattacaa ttgcctcata cggttttccc ttttcgtcaa
     6061 gacggactgc tttcccgtat aaccgtgagg ccatacgcac ctttcgcgaa tcaccaaact
     6121 tttttcggaa tgaccacatg ctgagaaacc ctttagtgcg tttcagcgac tcctttgtca
     6181 ttgttatttg aaccatgggt attttagaat cttgccgata gggaacacaa tagcacagct
     6241 tcctcaccat cccctgctgc tgttctgagg agcattttta ccattccggt aatagtggca
     6301 tcccttgggt actttgtgga catctacgat cttatcctca tccgggcatg gtcatcccca
     6361 tcaccttttc attccagttt gcgaaggagc ccctcgacgt catttaaggc gccgctgtgg
     6421 gtgcgatatg tctcgtcatc actttttacg ccctctacat caggaagaca cctttcataa
     6481 ggacctcaat tatgttgagg agtagggggt gaggaagtga tcggtgttat gctggtctta
     6541 tttgcaaagc ggtatatata acgtttgacg tttaacgtaa agcgttatat actatatgag
     6601 gtgataaaat cattcaaaaa caaagaaaca gagaacgtct atcaactgga gggttccggc
     6661 aaattgccgg gggatattca gcaggttgca ttgaggaaat tgcgcatgat aaataacgct
     6721 ataaatctgc ttgacttaag aatacctcct gccaacaagc tggaaaaact caaaggcgac
     6781 aggagggggc agcacagcat taggattaat gatcagtggc gcatatgctt cacgtggaga
     6841 gacggggatg cgtatgaagt gggaataaca gattaccatt agaaaggaga ccatgaaaaa
     6901 gaaattatat cccgtacatc cgggcgaaat cctttcggag gagtttctta agccgatggg
     6961 tctaagtcag aataggcttg ctcttaacat aggggtatcg gcacggcgaa taaatgagat
     7021 agttctcggg aaacggggga ttacggctga taccgcactc aggttagcaa ggttttttgg
     7081 aacatccgcc cagttctgga cagggctgca gtcgcaatac gatttggatg tgaccgcgga
     7141 agcgcttcgt ggacgcctgg agcgggaagt gaaggcatac gcgaaggctt tatgaatagg
     7201 ccatctgctc ggttatgata gcttcctagt gaattggtaa ttaactctgt gcagttcggc
     7261 accaacccga gggccaccat tgcgtccaca gcccaacttc atccgtggca tggtcatccc
     7321 catcatcttt tcattccagt ttgcg
//
LOCUS       DATKOU010000123        23178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2470399, whole genome shotgun sequence.
ACCESSION   DATKOU010000123 DATKOU010000000
VERSION     DATKOU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 23178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23178
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470399"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="VMU21_05675"
     CDS             <1..1721
                     /locus_tag="VMU21_05675"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HUO77051.1"
                     /translation="YEGEIFSKRDNYHITGLEIEKTGPKEYTLKTASFTTCDAPVPAW
                     CFKGSDVDAVVGERLKARSVTFNIIDVPVFYSPYLSSALGKERKTGFLTPVVGLVSSK
                     GVHLELPFYWVISENMDATFLPDIYTKTAVGEGLEYRFVEPDGSKGNFWLYHLKDKNF
                     ADPLWIARGTYDRDRESPVTGYLNLNYVTPVDLYNEYNPYIFSKQRLFDSLSYLNQTT
                     GRFLDSTGEVSAKFSGSRLYLDAQYLVDLQEGVNQSTILQKLPEAGYFINPRRIGPLV
                     FSLSSNAAEFWRDQGVSGQRVDIYPRFSYSFGSDIIIDQALGLRETAYFLNNADVTGS
                     SPHRESLDYTIIAHTRLTKNYGSFTHIIEPSLSYTFIPNAESSLPLFDSTELYTKTST
                     IQLALLNRFIDSKGEFLTVKLTQPYDTYLHGNSVLPLPYDTYLQGKSFQPLQLQVAIQ
                     RPITFRNETLLDVNTGSLEEVNSDITTPMPGKGSLSIGERYNRTQNILFFTFAANYSF
                     SKAVSAEGNFWYDAKSGSLSNVIAKLKYQQQCWGVTTVFTKSETHGVSVSLLFDLLGL
                     GTIKAF"
     gene            complement(1730..2251)
                     /locus_tag="VMU21_05680"
     CDS             complement(1730..2251)
                     /locus_tag="VMU21_05680"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HUO77052.1"
                     /translation="MALWITGLPGSGKSTIADEVKKLHPDFIMLRMDALRKVVTPEPT
                     YSDSERDIVYRCLVYTASVLTEHGHAVIIDATGNLRKWRDLARQVIPRYAEIYLKCPV
                     ELCIERERLRSETRGAPREIYQKGEAGWPVPGFSVPYEEPPHPELLIAADRTSVAKAV
                     EMIEGLLRRLQKH"
     gene            complement(2349..3893)
                     /locus_tag="VMU21_05685"
     CDS             complement(2349..3893)
                     /locus_tag="VMU21_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO77053.1"
                     /translation="MQNLRRISQGIFLVIFLFLFVQAESKGNDVLGYPAKIFLDFDPL
                     ILTTTVLAAHTAAKAFSLSLIMIVATLLLGRVFCGWICPLGTLNNMVGSLKKKRPERA
                     PKNWYRVKYYILAFILASSVFTLQLVGIMDPLSLLIRSLSVSVYPLFNYGVRAFFDAV
                     YNLNTKGVVDISEFIYSFLKKTILSFQQPFYNQAIFIGTLFFLVLGLNLVEKRFWCKY
                     LCPLGAFLGMLSKSSLLKRSVSEGCTSCGACATACQGNASPDRKDEWRDTECLYCWNC
                     DDICPQNTVSFGFAQKKAAGAMDLGRRRVVTSVLSGVIAVPLMRSVPLSKSGFSQPRL
                     IRPPGSLEEREFLKRCVKCGECMKVCITNGLQPTLIEAGLEGIWSPVLIPRIGYCEFR
                     CTLCGQVCPTGAIKRLELKDKMKVKIGLAMIDKGRCLPYAHATPCIVCEEVCPTPRKA
                     IWFEEAKAKDREGKEHVLKQPRVDLELCIGCGICEAKCPVLGQPAIYVTSVGESRSRE
                     NQLLLS"
     gene            complement(3903..4802)
                     /locus_tag="VMU21_05690"
     CDS             complement(3903..4802)
                     /locus_tag="VMU21_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HUO77054.1"
                     /translation="MDRREFLKRAALTGLGISLPGLGKVIRRLEASEKIDLVVARGDS
                     PSKITKAAVDAMGGIKRFISRGDVVVIKPNMGWDRLPEQAANTNPEVVATMVRLCLEA
                     GAKKIKVFDRPVNDPRRCYVQSGIADAVKPLGAELEYMDERKFRDMSIRGEALKSWPL
                     YTEVLEADKVINIPIAKHHSLAKLTLAMKNWMGVMGGSRRQIHQKLDESLADLGLVIK
                     PTLTVLDAVRILTANGPQGGDLKDVKKTDTVIVGIDQVAVDSFGATLFGMKGSDLGYV
                     RAGDKMGLGTMDISKLKIRRITA"
     gene            5083..5397
                     /gene="clpS"
                     /locus_tag="VMU21_05695"
     CDS             5083..5397
                     /gene="clpS"
                     /locus_tag="VMU21_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HUO77055.1"
                     /translation="MSDNELKSAEHREAETREEVKSPSLYKVLLLNDDYTTMDFVVQV
                     LEEVFHKAPAEATQIMLHVHKKGIGLCGIYTRDIAETKVATVHELARGKGFPLMCMME
                     KE"
     gene            5394..7643
                     /gene="clpA"
                     /locus_tag="VMU21_05700"
     CDS             5394..7643
                     /gene="clpA"
                     /locus_tag="VMU21_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HUO77056.1"
                     /translation="MISKELELTLEATIRDAQARHHEYLGVEHILFAVLHDEWGVEVI
                     THCGGSVEKIKAAIEDFFEQHIPKLSSDAGAYLQPTTGFHRVLQRALDHVQSSGKTEA
                     DAGDVLASICLEEDSHAQQFLRSEGITRLDVLNYISHGIPKTKEASGSGKDEETEVHD
                     EKGEKRIQNPLKLFAVSLTEKAQKGDLDPLVGRREELERTIQVLSRRRKNNIIFVGEP
                     GVGKTALVEGLALRVHKGQVPEVLKDTQIFALDMGGLLAGTKYRGDFEARLKATIKGV
                     ENIPGAILFIDEIHTIVGAGATSGGSLDASNILKPVLISGKLRCIGATTYEEYKNYFE
                     KDRALSRRFQKIEIQEPSIGETLSILRGLKAYYEEFHGIKYTEGALKGAAELSAKYIN
                     DKYLPDKAIDVIDEAGATLKLSQAFKRKKTVGLYEVEKVVAKMAKIPSRTISTSDMDK
                     LKVLKDELKRVVFGQDAAISSLVSSIKRARAGLGSPEKPIGSFLFLGPTGVGKTEIAK
                     QMALVLGVQFTRFDMSEYMEKHAVARLIGAPPGYVGFDQGGLLTEAIRKHPYNVLLMD
                     EIEKAHADIFGILLQIMDYATLTDNNGKKADFRNVIIIMTSNAGAQDMDRNVIGFGER
                     GVDTRAKGKEAIMKLFTPEFRNRLDAIITFNPLTTDIMKRVVDKFISELESQLRGKGV
                     TISLSDEARGWLAQKGYDPRYGARPLGRLIQEEIKDFLSEEILFGRLEKGGKVFIVLK
                     DGRLSFEYQ"
     gene            7738..8427
                     /gene="aat"
                     /locus_tag="VMU21_05705"
     CDS             7738..8427
                     /gene="aat"
                     /locus_tag="VMU21_05705"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HUO77057.1"
                     /translation="MPVFQLPDRIVFPSPEYAEEDGLLAVGGDLSEERLLLAYSAGIF
                     PWYSEGTPILWWSPDPRLILLPDELKVSRSLRQTINKGMYKVTMDKAFGEVIENCASV
                     SRKEGEGTWITAEMVTAYVRLHDSGFAHSVESWYGEELAGGLYGVSLSGVFFGESMFA
                     KRRDASKVAFVKLIQQLKEWDFRLVDCQITTEHLKRLGAREVSRLEFMSRLREALKRP
                     HRHGRWTQTMS"
     gene            complement(8534..10234)
                     /locus_tag="VMU21_05710"
     CDS             complement(8534..10234)
                     /locus_tag="VMU21_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUO77058.1"
                     /translation="MDIFQTVSQRIGSLEYLEEYPECERLEPLEEKSPGAKEIHWGES
                     EDEGEGEIEAEERVAFVTEEYDPIRMYLKEMGNFPLLKKEDEIELARQIEKGRERIIE
                     SIFPLPFVVEELLTYGALIRKGGASLSEFTQNEADSMETPEAEKRKFLAHLERVKDLY
                     QLRTRCLKRLHRKQRMSRPSPGKISLRTTRSLENNREKILENIKALRLKEVVIDTFIE
                     KLDNAVRQIRELNNKRVLLEKRLDDLNYDIKGKKKNPLRPSFRKTPVNPSPPPSIGHE
                     NFNTHPETLMKKYREYGREVTRTECFIGLPYSKMKTVMNILTDARADAFAAKNAMIEA
                     NLRLVISIAKRYIGKGLSFPDLIQEGNIGLMRAVEKFEYRRGYKFSTYATWWIRQAIT
                     RALADQSRTIRIPVHLLDLKNRIAKVTRELVQELGAEPSPEEIASRVNMPPEKVRTIL
                     KIAREPLSLEAPTSEDETSHLSDFIEDRTTPSPLESLISDDLRYHINKIVTTLNPKEE
                     RIIRKRYGIGEEASCTLEELGYEFDVSRERIRQIEVKAVKKLRYPVKSMWLREFAPPL
                     "
     gene            complement(10496..11128)
                     /locus_tag="VMU21_05715"
     CDS             complement(10496..11128)
                     /locus_tag="VMU21_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO77059.1"
                     /translation="MIKILIADDHTIVRKGLKQIIAETSDMIVTDEACDGQEVLNKTR
                     KDSFDMVLLDISMPGRTGLDILRELKAENPKLPVLVLSMYPEEQYAVRVLKAGASGYL
                     TKESAPDELIAAIRKVSMGKRYVTPSLAEKLAFNLQINSDKPLHENLSDREYQVMCMI
                     ASGKTVGEIAEKLSLSAKTISTYRARILEKMKMKNNAEITHYAIQNRLVA"
     gene            complement(11225..13726)
                     /locus_tag="VMU21_05720"
     CDS             complement(11225..13726)
                     /locus_tag="VMU21_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO77060.1"
                     /translation="MTDEPRRGKMDVARHSLEKLDRLASFPELNPNPIIEVDLAGVVH
                     YINPAAMNLFHDLPAKGALHPFLADFDSLVSVFTHEGKHSHVRELKADNVWYEQVLHF
                     ARGNNVIRIYSHDITARKQAEEVLQATKEELEVRVQERTAELLRVNTELEEEIAQRKR
                     AIGALSGQSRILEAFFDHTITPLVFLDRDFNFIRVNTAYANACQRDVSDFAGHNHFEF
                     YPHKENEGIFKRVVATKVPYQAVARPFSFPDHPEWGVTYWDWNLVPVLDSRGEVDFLV
                     FSLKDVTEQKEFQDRMLTHNTLLQLFSHSFTRNEYLYEVVNLMAKWSGSRCVGIRVLE
                     KNGNIPYDAYRGFSSQFWEVENRISLHHDQCACVRVIAGTPDPQDASAMTPGGSFYCN
                     NTIEFVKTLTPEESSRYRGTCVGSGFRSVAVVPIRYSEKVLGAIHLADEREGMVPLKI
                     IQLIEVVTPLIGEAIYRFSIEDELWRNYEALQRSEKSLSEAQRIAGLGNWEWDLLTNE
                     LRWSDETYRIFGLTPQQTATTYVAFLNYVHPDDREFVKKSVNEAIYDRQPYNIEHRIV
                     LPNGLLRVIHEQGEVRFDDRDEPINIVGTVQDITERKRAEEELTYSREQLRNLSMHLQ
                     SVREEERTTIAREIHDELGQVLTALKMDLSWLDKKHRGDKSLAERTKSMLSLVDSTIQ
                     TVKRISSELRPGVLDHLGLAAAIEWQAKEFEKHTGIACDVSVDPDDIVLDRHHSTTIF
                     RIFQEALTNVARHAHATKVRISLHEKAEGIALHVEDDGRGIKKKQLNSPQSFGLIGIR
                     ERVHFLNGEIKIKGIRNKGTSLTIHIPHGKKAAQE"
     gene            complement(14172..14819)
                     /locus_tag="VMU21_05725"
     CDS             complement(14172..14819)
                     /locus_tag="VMU21_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77061.1"
                     /translation="MEDVNERLLNFFTEHHKPGTIGLVGSNDPIGQAVREAQRAVTGD
                     KKPSLWSHSFILGELRLDRRGPRNARSESPYIFESDLKIKLFKPQLRNGAQENWIGKW
                     CGTTVENAALIDFGLSEDEKDDVLATALQLVDEQVLYPIQELLGTWWAIIARRQWLEN
                     PFNDPHAMYCSSFVRHCYQEAGRDFLGTEISVSNTTPEDIARGGTEVDAIRTYTP"
     gene            complement(15174..16610)
                     /locus_tag="VMU21_05730"
     CDS             complement(15174..16610)
                     /locus_tag="VMU21_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUO77062.1"
                     /translation="MIMPFRGYLRDYSLPKILIELNRNRETGTLSITTPRFTKKIYIR
                     GGNVIFASSTFEDDRLGEMLIKAGKITMGQYDKSVELLKSTGKRQGAILVELGYITPK
                     DLFWGVKYQVKEIIYSLFQLDEGEYEFVEDIPPDEVITLKISTNNLIYEGVNKINNLT
                     RIRRDMPDAHTIFTLNEDPSGIFRGIDLSPQDAKILSLVDGTRSINQVIEQSRMNSFD
                     VMRTLYVLWSTGFITQKETAEHGLDVQETINVEEILVSMAEEEKNLKNKVEELYAKLP
                     RMSPAEMLQANEKTGPEEIKKTYQRMTKEFNPDRVHASGDATLRTKVTVIFDTITQAY
                     NSLKDEKRRMEFFGALEKIPKKEEDTETVLIEEQFRRGVEEFKNGNFWGAVEIFKWVT
                     KNNPRNPKAWSYLSLALSKMPHRMKDAEEALLESIKLEPYSGEHFANLGILYLKGGHK
                     KKAELQFEKALKLDPANAKAKKGLEQAQ"
     gene            complement(16611..17117)
                     /gene="amrA"
                     /locus_tag="VMU21_05735"
     CDS             complement(16611..17117)
                     /gene="amrA"
                     /locus_tag="VMU21_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HUO77063.1"
                     /translation="MDPLVELAKRAVEELILKKRGIQPPDPLTPVMSEKAGVFVCLKK
                     EGQLRGCIGTFLPCCGNVAEETIKNAICAATEDPRFPLVAKEELPFITYSVDVLSPPE
                     KISDPKELDPKKYGVIVVKGGMRGLLLPDLEGVETVEEQVRIAKMKAGIDPSESADIY
                     RFEVRRYH"
     gene            complement(17117..18088)
                     /locus_tag="VMU21_05740"
     CDS             complement(17117..18088)
                     /locus_tag="VMU21_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUO77064.1"
                     /translation="MFTDTFSLYTEELGLVEKNLRTIFTSDASLLPVIGEHILGGGGK
                     RLRPLFLLLSADLARYKGEKRTVLASIIEAIHTASLLHDDVVDSAELRRGKQTGHMIW
                     GNQIVILVGDFLYSNALRLAVLQKNQRIMEALSEATTRMTEGEILQLTRVADPDITEE
                     EYYRIISAKTGALISAACRIGGILGGLSPEKEDALGRFGMKTGIVFQMVDDILDYMAD
                     EKGLGKKLGKDLLEGKITLPLIYLLGVASGEEKAEIKGITKNGVSGEKELKRILHLFG
                     KYRVIDESLRIAQGLVSEAKSELEIFAPSPERDALRAMSDHALVRDR"
     gene            complement(18088..19212)
                     /gene="mtnA"
                     /locus_tag="VMU21_05745"
     CDS             complement(18088..19212)
                     /gene="mtnA"
                     /locus_tag="VMU21_05745"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HUO77065.1"
                     /translation="MVKTIEWRDDKVIMLDQSRLPSEVRFIECTDYAMVAEGIRELQI
                     RGAPAIGIAAAMGIALGAKALNAETYDHFMQMLEPVFSAMLATRPTAVNIQWAVKRIK
                     EMLTREKDRPVPALKELLVEEAKRILQEDIEVNRSIGRFGAELIKDGDTILTHCNAGA
                     LATGGYGTATAPMLYAKEQGKRMQVVADETRPVLQGARLTAWELMQEGIPVTLITDNS
                     AGSLMRKGKIDLIIVGTDRTVRNGDVANKIGTYPLAVLAKEHNIPFYVAAPTSSIDLS
                     IPTGDEIPIEERDPSEVTHIYGRISYCEGSAEDESCENERGQKGKNVPRVSWGAVRIA
                     PEGVAVRNMAFDVTPARYITAIITEKGIFRPEDIKTMDRH"
     gene            complement(19213..20454)
                     /locus_tag="VMU21_05750"
     CDS             complement(19213..20454)
                     /locus_tag="VMU21_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HUO77066.1"
                     /translation="MRRWQKIVMFASIIGPGIITANIDNDASGITTYSVAGARFGYGL
                     LWTLLPTTVALVVIQEMIARLGVVTGKGLSYLIRESYGVKTAFFMMVGLLVANFGTTV
                     ADFAGWAASMEILGLSKYIMVPLGAISIWILVTKGSYRFVEGVLLVACLVYIGYIVSG
                     VMAKPDWGGIMKNTFSPRLQWNSEYIMLSIAIIGTTITPWMQFYLQSSIVEKGITPED
                     YKVSRLDVITGCLITDIISFFIIVTCGALLFPHGIRINEATEAAIALKPLAGDYASLI
                     FALSLANASLLGAIIVPISTAYYICEAMGWEAGVNKNFKEAPQFMWIYTLMIVLSALL
                     ILIPEAPLVLLMVLSSLVNGLLLPFVLIYALSLINNKELMGKYANPKSYNYIAWGTVA
                     VIICLTLLLILTIIFPLGGKA"
     gene            20597..21025
                     /locus_tag="VMU21_05755"
     CDS             20597..21025
                     /locus_tag="VMU21_05755"
                     /inference="COORDINATES: protein motif:HMM:NF021896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase DsbC N-terminal
                     domain-containing protein"
                     /protein_id="HUO77067.1"
                     /translation="MRRLLFSVVMALMLLPVSYSYGFSEKGQDCSKCHTLTKDEAATL
                     MKSFGQEVKVLEVLTSPIKGMWEVDVDAGGKKGLVYIDFSKNYLVSGSIISLKNKTNL
                     SQERFTELSKVDVSQIPLDDAIVMGEKDAKYKIVVFSDPD"
     gene            21038..21361
                     /locus_tag="VMU21_05760"
                     /pseudo
     CDS             21038..21361
                     /locus_tag="VMU21_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
     gene            21434..21517
                     /locus_tag="VMU21_05765"
     tRNA            21434..21517
                     /locus_tag="VMU21_05765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21468..21470,aa:Leu,seq:cag)
     gene            complement(22904..>23178)
                     /locus_tag="VMU21_05770"
     CDS             complement(22904..>23178)
                     /locus_tag="VMU21_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HUO77068.1"
                     /translation="TERKMAEEALRESETALRYSQKNLQMLAGRLISAQEEELRRLSR
                     ELHDDLTQRLAVLAIEASKLELELNKAPQSLSGPSQKISQMTYSPG"
BASE COUNT         5638 a         5720 c         5542 g         6278 t
ORIGIN      
        1 tctatgaagg cgagattttt tcaaagaggg acaactacca catcactgga cttgagatag
       61 agaagaccgg accaaaggag tacacattga agacagcgtc attcaccacc tgtgatgcac
      121 ctgtccctgc gtggtgtttc aaaggctctg atgttgatgc tgttgtgggt gaaaggctca
      181 aggcgagaag tgtcactttc aacataatag atgttcctgt attttactct ccctacctct
      241 cttcagcgtt gggcaaagaa agaaagacag ggtttctgac cccggttgtt ggcttagtaa
      301 gttccaaggg tgttcatctt gagctgcctt tttactgggt gatctctgag aatatggatg
      361 ccacattcct gccggatatt tacacgaaga ccgctgtggg cgagggtctg gaatacagat
      421 tcgttgagcc tgacgggtcc aagggcaatt tctggttgta tcatctcaag gacaagaact
      481 ttgctgaccc gttatggatc gcaagaggaa cttacgacag agaccgtgag agcccggtca
      541 cgggatacct caatctgaac tatgtaacac cggttgatct gtataatgag tataatccct
      601 atattttttc gaaacagcgt ctctttgatt cgctttctta tcttaaccag acgaccggga
      661 ggtttttgga ctccaccggg gaggtatctg ctaagttcag cggttcacgt ctttatcttg
      721 atgcccagta tctcgttgac ctgcaggagg gcgtaaatca gtccaccatt ttgcagaagc
      781 tgcccgaagc gggatatttc atcaacccgc gaaggattgg cccgctggtg ttttctctgt
      841 catccaatgc cgcagaattc tggagagacc agggtgtttc cggtcagagg gtggatatct
      901 acccaaggtt ctcctattct tttggaagtg acattatcat cgatcaggcc ttgggattgc
      961 gggaaactgc atattttctt aataatgccg atgtgacggg aagttcgcct cacagggaaa
     1021 gcctcgacta taccattatc gcacatacaa gactcacaaa gaactatggt tccttcaccc
     1081 atatcattga accgtcgctg agctatacct ttattccgaa tgccgaatca agtcttcccc
     1141 tctttgactc cacagagctt tacacaaaga cctccacaat acagctggcc cttctcaaca
     1201 ggttcataga cagcaaaggt gagttcctga ccgtgaagct cacccagcca tatgacacgt
     1261 acctccacgg aaactccgtt ctgccgctgc catatgacac atacctccaa ggaaaatcct
     1321 ttcagccact gcaattgcag gttgccatcc agagaccgat aaccttcagg aatgagacgt
     1381 tgcttgacgt aaacacaggc agccttgaag aggtcaattc tgatataacc acgccaatgc
     1441 ccggcaaggg atcactgagc ataggcgaaa gatacaatag aacgcagaac atactcttct
     1501 tcacctttgc agcgaattat agtttctcga aggcagtttc tgcagagggg aatttctggt
     1561 atgacgcaaa aagcgggagc ctcagcaatg ttatcgcaaa actgaaatac cagcagcagt
     1621 gctggggggt aaccacagtg ttcaccaaga gcgagactca tggtgtcagc gtctctttac
     1681 tttttgacct cctggggctc gggaccatca aggcattttg agaaatgtat cagtgtttct
     1741 gtaatctcct cagaagacct tctatcatct caaccgcttt ggcgacggaa gttctgtctg
     1801 ctgctatcag aagttcggga tgaggaggct cttcgtaagg aacggagaaa cccggaaccg
     1861 gccagccggc ttcacctttt tgatagattt cccttggcgc cccgcgggtt tcggagcgta
     1921 gtctctccct ctcgatacat aattcaaccg gacatttcag gtaaatttcg gcatagcgcg
     1981 ggataacctg ccgcgcgagg tcccgccatt tccttagatt ccccgttgcg tcgatgatta
     2041 cggcatggcc atgttccgta aggactgacg cggtgtacac aaggcaacga tatacaatat
     2101 ctctttcaga atcggaatag gtcggctcag gggtaacgac ttttctcaat gcgtccatgc
     2161 ggagcataat gaaatccgga tggagttttt ttacttcatc cgcaattgtg ctttttccgc
     2221 ttcctggaag gcctgtgatc catagtgcca ttgaactcat atattgagag tttatcataa
     2281 tgcaggacta tgaaaaagcc gtcagatgag gcaattcgtc ctgcacttaa ggatttgttc
     2341 ttgcagactc aggataacaa taattgattt tcccttgacc ttgattcacc cacgctggtc
     2401 acataaatcg caggctgccc gaggacaggg cattttgctt cgcagatgcc gcagccaata
     2461 cagagttcaa gatctacccg cggctgtttg agaacgtgtt ctttgccctc cctgtctttt
     2521 gcctttgcct cttcaaacca tattgccttt ctgggtgtgg ggcagacctc ttcgcatacg
     2581 atacacgggg tggcatgggc atagggaaga catctgccct tgtcaatcat ggcgagccct
     2641 atctttacct tcatcttatc ctttaattcc agtctcttaa tggcacctgt gggacatacc
     2701 tggccgcaga gggtacaccg gaattcgcaa taccctattc tcggaatgag cacaggtgac
     2761 cagatcccct caagtcctgc ctcgatgagg gttggctgca atccattggt aatgcatacc
     2821 ttcatgcact cgccgcattt cacacatctc ttgaggaatt ccctttcctc gagagagccg
     2881 ggtggcctga tgagcctcgg ttgggaaaac cctgacttcg agaggggtac tgacctcatc
     2941 aaagggacgg caatcacacc cgagagcacg gaggtgacta ccctcctcct gcccagatcc
     3001 attgccccgg cggccttctt ttgtgcgaat ccgaagctca ccgtgttctg gggacagata
     3061 tcatcacagt tccagcagta aagacactcg gtgtccctcc attcgtcttt cctgtcagga
     3121 gaagcatttc cctgacaggc cgtggcgcag gcgccacacg aagtacaccc ctcgctcacc
     3181 gacctcttca gcaaggaaga tttcgagagc attcccagaa aggcaccaag gggacagaga
     3241 tacttgcacc agaaccgctt ttccacaaga ttgagaccaa gaaccaggaa gaagagagta
     3301 ccgatgaata tcgcttgatt gtaaaaaggc tgctgaaagg agaggatggt ttttttcaaa
     3361 aaggaataga tgaactccga tatgtccaca acccctttag tattgagatt gtaaacagca
     3421 tcaaagaatg ccctcacccc gtaattgaat aagggataca cactcaccga gagcgacctg
     3481 ataaggaggg agagaggatc catgatgccc accaactgaa gggtgaacac agaagacgca
     3541 aggatgaatg ccaggatata gtactttacc ctgtaccaat tcttcggggc tctctcagga
     3601 cgtttcttct tcagcgaccc caccatatta ttcaatgttc ccaggggaca tatccatccg
     3661 cagaataccc ttccaaggag gagtgtcgcg acaatcatga tgagggagag ggaaaaagcc
     3721 ttggcagcag tgtgggcagc gaggactgtt gtggttaata tcaggggatc aaaatcaaga
     3781 aagatttttg cagggtatcc cagtacgtca ttcccctttg actctgcctg aacaaagaga
     3841 aagaggaaaa tgactaaaaa tatcccctgg gagatccttc taaggttctg catagagggg
     3901 tctcaggccg tgattctcct gatcttgagc ttagaaatgt ccatagtacc gaggcccatc
     3961 ttgtctcctg ccctcacata tccaaggtca cttcccttca tgccaaaaag ggtcgcacca
     4021 aaggagtcaa cggcaacctg atcaatcccc acgatgaccg tatctgtctt tttcacgtcc
     4081 ttcaaatccc ctccctgggg accattggca gtgagtatcc tgaccgcatc cagtacagtg
     4141 agggtgggct tgatgacgag tcccaggtca gcaaggcttt catccagttt ctggtgtatc
     4201 tgcctcctgg atccccccat gacgcccatc cagttcttca ttgccagggt aagcttcgcg
     4261 aggctgtggt gttttgcaat ggggatgttt atcaccttat cggcctcaag gacctctgtg
     4321 tagagcggcc atgatttgag ggcctcacct cttatgctca tgtccctgaa tttcctttca
     4381 tccatatact ccagctctgc gcccaggggc tttaccgcat cagcaatacc gctctgcaca
     4441 tagcacctcc tggggtcgtt cacgggccgg tcaaatacct ttatcttctt tgcacccgcc
     4501 tcaagacaga gtcttaccat ggtcgcaact acctcagggt tggtattggc cgcctgctca
     4561 gggagtctgt cccatcccat gtttggtttt atcaccacca cgtctcccct ggagatgaat
     4621 ctctttatgc cgcccatcgc atcaacggct gcctttgtaa tctttgaggg agaatcacca
     4681 cgggcaacaa caagatcaat cttctctgac gcttccaacc ggcgaataac tttcccgagg
     4741 ccgggaaggg aaatccctag acctgtaagc gctgctcgct tcagaaactc tctgcggtcc
     4801 atacttgttc ctttctctaa tgctactgcc cgggcgttaa agcaaatcag ttcgtcctca
     4861 aggactccca cagaggaatc ggggattccc aacaaatgaa acagcagcca acgcaatgac
     4921 cataatttat attctcaata aatgtatggg cattgtcaat tcgaaacaag ttttttctcc
     4981 attaaagggg agatgagttc atatcatatt gtttcatcca gatacgcctt agtgaaaatc
     5041 tgctagaata aatataaaga cattaatgaa ctggccggaa gaatgagtga caatgaactg
     5101 aagtctgcag agcatcgtga ggcagagacg agggaggagg taaagagtcc gtccctctat
     5161 aaggttcttc tcctcaatga tgattacacc acaatggatt ttgtcgttca ggtccttgag
     5221 gaggtctttc acaaagctcc tgctgaagcc acacaaatca tgctccacgt tcataagaag
     5281 gggataggat tgtgtggaat atatacgcgg gatattgcag agactaaagt ggccacggtg
     5341 cacgaactgg cacggggaaa gggttttccc cttatgtgca tgatggagaa ggaatgatca
     5401 gcaaggaact ggaacttacc ctggaggcta cgatacggga tgcacaggcg aggcaccatg
     5461 agtacctcgg cgtggaacat atcctgttcg cggttctcca cgacgaatgg ggagttgagg
     5521 taataacgca ctgcggcggt agtgttgaaa agataaaggc agccatcgaa gatttttttg
     5581 aacaacacat cccaaaactt tcctccgatg ccggtgcata ccttcaaccg accacgggtt
     5641 tccatcgtgt actccagagg gcgctggacc atgtgcaatc atccggaaaa actgaggcag
     5701 atgccggtga cgtcctggca tccatttgct tggaggaaga ttcgcatgcc cagcagttcc
     5761 tccggtcaga aggcatcaca aggctggatg tgctgaacta tatctcccac gggataccaa
     5821 agacaaagga agcgtcagga agcgggaagg atgaggagac agaggttcat gatgaaaaag
     5881 gggagaaacg gatacagaac cctctgaaac tctttgccgt aagtttgacc gagaaggccc
     5941 aaaagggcga tctggatcct ctggtaggcc ggcgggaaga gcttgagagg accatccagg
     6001 tgctcagcag gagaagaaaa aacaatatca ttttcgtggg tgagccgggg gtgggaaaga
     6061 ccgctctcgt ggaaggcctt gcattaaggg tgcataaggg gcaggttcct gaggtgttaa
     6121 aggacaccca gatatttgcg ctggatatgg gagggctttt ggcagggacc aaatacaggg
     6181 gcgacttcga ggcacgtttg aaggctacca taaagggcgt tgagaatatt ccgggcgcca
     6241 ttctttttat cgacgaaatc cataccatcg ttggtgcggg ggccacaagt ggtggctccc
     6301 ttgatgcatc caacattctc aagcctgtgc ttatttcagg gaagttgcgc tgtattggcg
     6361 cgaccaccta cgaggaatac aagaattact tcgaaaagga ccgtgccctt tcgcgtcgtt
     6421 tccagaagat tgagattcag gaaccgagca ttggagaaac cctctctatt ctgaggggac
     6481 tgaaggcata ttacgaggaa ttccatggca taaaatatac cgagggggct ctcaaggggg
     6541 cggccgaact ctctgcgaaa tatatcaatg acaaatatct ccccgataag gccattgatg
     6601 tcatcgacga ggcaggggca acgctgaaac tctctcaagc attcaagaga aagaagaccg
     6661 tggggctcta tgaagtggaa aaggtggttg cgaagatggc gaagatcccg tctcgcacca
     6721 tatccacatc tgatatggac aagctgaagg tattgaaaga tgaattgaag agggttgtgt
     6781 tcggacagga cgctgccatt agttccctcg tctcctccat taagagggcg cgtgcgggac
     6841 tgggttcacc cgaaaaaccg atcgggtctt ttctcttcct ggggcccacg ggagtgggga
     6901 agacagagat cgcaaaacag atggcattag ttctcggtgt ccagttcacc cgttttgata
     6961 tgagtgaata tatggaaaaa catgctgtcg cccgtttaat aggcgcaccg ccggggtatg
     7021 tggggtttga ccagggagga ttattgacag aagccatccg aaagcacccc tataacgtcc
     7081 ttcttatgga tgaaatcgaa aaggcacacg ctgatatctt cggcatcctc ctccagatta
     7141 tggattatgc cacgttaact gataacaacg ggaaaaaggc ggatttccgg aatgtcatca
     7201 tcatcatgac ctccaatgcc ggagcccagg atatggacag gaacgtcatc ggctttgggg
     7261 agaggggtgt ggatacgcgc gcaaaaggaa aggaagcgat catgaagctc ttcactccgg
     7321 agttcagaaa caggctcgat gccatcatta cctttaatcc tttaactacg gatatcatga
     7381 agagggtggt ggacaagttc ataagcgagc ttgaaagcca gctgaggggc aaaggcgtca
     7441 ccatctccct gtctgacgag gcgaggggct ggcttgcaca aaagggatac gatccccgtt
     7501 acggagcgcg tccgttggga agacttattc aggaggagat caaagacttt ctctcagagg
     7561 agatcctttt cgggagattg gagaaagggg gaaaggtctt tatcgttttg aaagacggcc
     7621 gcctgagttt tgaatatcaa taaaacgatt gtacaatacg tgctgtcatg cgttctcttt
     7681 ttctcctgaa ctctttatgg gatgttctct atctgttcgg caaaaggtat aggttttatg
     7741 ccggtctttc aactccctga cagaattgtt tttccatccc cggaatatgc tgaagaggat
     7801 ggcctccttg ccgtgggggg cgatctcagc gaagagcgcc ttttacttgc gtattcagcg
     7861 gggattttcc cctggtattc agagggcact cctattctgt ggtggtctcc tgatccccgt
     7921 ctcattctgc tccccgatga actcaaggtg tccaggagtc tgaggcagac cataaataaa
     7981 ggcatgtata aggtgactat ggacaaggca tttggtgagg taatagagaa ttgtgcatcc
     8041 gtatccagga aagagggaga aggcacatgg atcaccgcag agatggttac cgcatatgtt
     8101 cgtcttcacg attcagggtt cgcccactct gtggaaagct ggtacgggga agagcttgca
     8161 ggagggctct acggggtttc cctgagcggg gtctttttcg gggaatccat gtttgcgaaa
     8221 aggcgtgatg cctcgaaggt tgcctttgtc aagctcattc agcagctcaa ggaatgggac
     8281 ttccggctgg tggattgcca gattaccacc gagcacctca agaggcttgg agctcgggag
     8341 gtctccaggt tggaattcat gagcaggtta cgagaagcac tcaaaagacc tcatcggcat
     8401 ggtcggtgga cccaaacgat gtcataatgt ggtgaaatga ttccctctac cacctcttgt
     8461 cagcgatcat ggtgaattat caaaaatgaa aacccccatc gccttattgc cctctgcctc
     8521 gtgagctcat ttgttaaagg ggtggggcaa attctctcaa ccacatgctc tttacgggat
     8581 acctcagttt ctttaccgct ttgacctcga tctgtcgtat cctctcccgt gagacatcaa
     8641 actcgtaacc gagttcctcc agggtgcaag atgcctcctc tcctatgcca tatcgttttc
     8701 tgataatcct ctcctctttg ggattgaggg tggtcacaat cttattgata tggtatctga
     8761 ggtcatcact gattaggctc tcgagagggg aaggtgttgt cctgtcctca atgaagtcgc
     8821 tgaggtggct cgtctcgtcc tctgaggtgg gagcctcgag ggaaaggggc tccctcgcta
     8881 tcttcaatat ggttctcact ttttcagggg gcatgttgac cctggaggcg atttcctcag
     8941 gggaaggctc ggctccaagt tcctgtacga gttccctggt gaccttcgca atgcgattct
     9001 ttaaatccag caaatgaaca ggtatgcgga ttgtccttga ctggtcagcc agggcccttg
     9061 tgatcgcctg cctgatccac caggtggcgt aggtgctgaa tttatacccc ctccggtatt
     9121 cgaatttctc cactgccctc attaatccga tgttcccctc ctgaataaga tcaggaaagc
     9181 tcagcccctt cccaatgtat ctcttagcaa tgctgatcac gagcctgaga ttggcctcta
     9241 tcattgcatt ttttgcggca aatgcatctg cgcgggcatc agtaaggatg ttcataacag
     9301 ttttcatctt gctgtacggt aacccgatga agcattcggt tctcgtgact tctctgccat
     9361 actcccggta tttcttcatc aatgtctcgg gatgagtatt gaaattttca tgccctattg
     9421 acgggggagg ggaggggttt accggggtct ttctgaacga tgggcgcaga ggattcttct
     9481 tctttccttt tatatcgtaa ttgaggtcgt caagtctttt ttcaagtaat accctcttgt
     9541 tattcagttc tcgtatttgc cgcaccgcat tatccaattt ctcgatgaat gtgtcgataa
     9601 caacctcttt gagccttaat gccttgatat tctccaagat cttttcacgg ttgttctcaa
     9661 gagaccttgt ggtacgaaga gatatctttc ccggggaggg gcgactcatc ctctgttttc
     9721 tgtgtaatct cttaaggcac ctcgttctca gctgatacaa atccttcacc cgttccaggt
     9781 gagcaagaaa tttccttttt tcagcctcgg gtgtttccat tgagtcagct tcgttctgtg
     9841 tgaactctga gagggatgca cctccttttc gtatcaaggc accgtaggtg aggagttcct
     9901 ccactacaaa agggaggggg aagatagact ctatgattct ttctctccct ttttctattt
     9961 gccgcgccag ttctatttca tcttctttct taaggagagg gaaattgccc atctctttaa
    10021 ggtacattct tatcgggtcg tattcttcgg tcacaaaggc aaccctttcc tcagcctcta
    10081 tttccccctc accctcgtct tcgctctcgc cccagtgaat ctctttggca ccagggcttt
    10141 tctcctcaag tggttccagg cgttcacatt ctggatactc ttcaagatat tcgaggctgc
    10201 cgattctttg gctcactgtt tgaaatatat ccattttcct tacccctttc tcttgcgaca
    10261 attataaaaa ccttctcgaa ttattttttt gattttcaat tagttaaggt gaaaaggaac
    10321 ttcttcacta tataccattg gaataaaatg ggaaatcagc ataaaactga tttttttgta
    10381 ggagactcaa cgtatttttt gtaagtcttt gtcctacaag gaggacacgc tagcattcaa
    10441 atgtctgaag aagtgttaag agaataagga tgaaaaaaat ctactgggtt atatcttagg
    10501 ctaccaagcg gttttgaatg gcataatggg taatctccgc attattcttc atcttcatct
    10561 tttccaaaat acgggcgcga taggtgctga tagtttttgc gctcagtgaa agcttctcgg
    10621 caatctcacc caccgttttc ccggaagcaa tcatgcacat tacctggtat tcgcggtcgg
    10681 aaaggttctc atggaggggc ttatcagaat tgatctgaag gttaaaggcc aatttttctg
    10741 ccaatgaagg agttacatac ctcttcccca tagatacctt tcgtatggct gcaatcaatt
    10801 catcgggagc gctctctttg gtaaggtatc ccgaggcacc agccttgagg acgcgcactg
    10861 catactgctc ttcggggtac atgctgagca ccaggacagg gagttttggg ttttcggcct
    10921 ttaattctct caggatatca agcccggttc tccctggcat ggaaatatca aggagaacca
    10981 tatcaaaact gtcttttctt gtcttgttca gtacctcttg tccgtcgcac gcttcgtcgg
    11041 tgacaatcat atcagaagtt tctgcaatga tctgcttaag tcctttacgt acgatagtgt
    11101 gatcatctgc aatgaggatt tttatcatac taacacgtag tatattttaa attctggtct
    11161 tcactatcag acaaattcta attattttgt cggttattat cttacaagcc cggatggtag
    11221 aacgtcactc ttgagccgct ttttttccgt ggggtatatg tattgtgaga gaggtccctt
    11281 tattgcgaat gccctttatt tttatctctc cgttcaaaaa atgcacgcgt tcccggattc
    11341 ctatcaggcc gaacgattgg gggctattca actgtttctt tttgatcccc ctgccatcat
    11401 cttcgacgtg caacgcgatc ccttcggctt tttcatgtaa cgatatcctc acctttgttg
    11461 cgtgtgcgtg gcgggccaca ttggtcagcg cctcctggaa gatacggaaa atggttgtgg
    11521 agtgatgtct gtccagtaca atatcatcgg ggtccacact cacgtcacag gcgatgccgg
    11581 tatgtttctc gaactctttt gcctgccact caatggcagc agcgagtccg agatggtcca
    11641 gcactcctgg cctcagctct gaagaaatcc ttttcactgt ctggatggtg gaatcaacca
    11701 ggcttagcat tgattttgtt ctctctgcga gagacttatc gcctctgtgt ttcttgtcaa
    11761 gccaggacaa atccattttc agggcggtca gcacctgacc cagttcatca tgaatttcgc
    11821 gtgcgatggt ggtcctctct tcttccctca cggactgcag atgcatggag aggttacgca
    11881 gctgttccct ggaataggtg agctcttcct ccgccctttt acgctctgtg atgtcctgta
    11941 ccgttcccac aatgttgatc ggctcatccc tgtcgtcaaa tctgacttca ccctgttcgt
    12001 ggataaccct tagcagcccg ttggggagta ctatccggtg ctcgatattg tagggctgcc
    12061 gatcataaat ggcttcattc accgactttt tcacaaattc cctgtcatct gggtgcacat
    12121 aattcaggaa ggcgacgtaa gtggttgcgg tctgttgcgg ggtcaatcca aaaatacgat
    12181 aggtctcatc ggaccagcgc agttcatttg tcaggaggtc ccattcccag tttcccagcc
    12241 cagcgatccg ttgggcttca gaaaggcttt tctcactccg ctggagagcc tcgtagtttc
    12301 tccagagttc atcttcaatg gaaaatctgt agatggcctc tccgatgaga ggggtgacaa
    12361 cttcaataag ctggatgatc ttcaggggaa ccatcccttc cctttcatcg gcaagatgaa
    12421 tggctcccaa taccttttca ctgtaccgga tgggcaccac ggccaccgac ctaaatccgc
    12481 tgcccacaca cgttccccga tacctcgaag actcctccgg ggttaaggtc tttacgaatt
    12541 cgatagtatt attgcagtag aatgaaccac cgggggtcat ggccgatgcg tcctgcgggt
    12601 caggggtccc cgcgattacc cttacgcagg cacactggtc atgatggagc gatatccggt
    12661 tctccacctc ccaaaattga ctactgaagc ccctgtacgc atcatacggg atatttccgt
    12721 tcttttccaa tacccttatg cccacgcacc tgctgccgct ccatttagcc atcagattta
    12781 ctacctcata aaggtactcg ttccgcgtga atgaatggga aaagagctgt aaaagggtgt
    12841 tatgggtgag catacgatcc tggaactctt tttgctcagt cacatcctta agggagaaga
    12901 caagaaagtc cacctcacct ctgctatcaa ggacagggac taggttccaa tcccaatacg
    12961 tgacccccca ttcgggatga tcgggaaagg aaaaaggtct tgcaacggcc tgatagggaa
    13021 ctttcgttgc aactactctt ttgaatatcc cctcgttttc tttatgggga tagaattcga
    13081 aatggttatg tcccgcgaag tcggacacgt cacgttggca tgcgttggcg tatgctgtgt
    13141 tgaccctgat aaaattaaaa tctctatcga gaaataccag gggagttatg gtatggtcga
    13201 aaaatgcttc taatatcctt gactgcccgc tcagggctcc aatagcacgt ttgcgttggg
    13261 cgatttcttc ttccagttct gtattgaccc ttaaaagttc ggcagttcgt tcctgaacgc
    13321 gcacctccag ttcttccttc gtggcttgca gcacttcttc agcctgctta cgcgcagtga
    13381 tgtcgtggct atagatgcgt atgacattat tgcccctggc aaaatgcaat acctgctcat
    13441 accagacatt atccgccttc aattcacgga catgggaatg cttcccttcg tgggtaaata
    13501 cactaaccaa cgagtcaaaa tccgcaagaa aagggtgaag ggctcccttt gccggcagat
    13561 catgaaagag gttcatcgct gcggggttta tgtaatgaac aactcctgct aaatccacct
    13621 caataatggg gtttgggtta agttcaggaa aggatgcgag tctgtcaagt ttttccaggg
    13681 aatgcctggc cacgtccatc ttcccccggc gtggttcatc tgtcatggcg attttccctt
    13741 ttgggtttga atgaacgata cgacacgcgt gaagcccccg gcaagagaaa gagaaaaggg
    13801 ataaagttga aggcccgctg cagtgcgctc aggcgaggac aatgcccggg acacaaggct
    13861 ttattgttac cagagacaaa gggttgcttc tctccgttgc tcattgtacg tcaagccgct
    13921 ctctttgcac gcgactttat tatagcaaaa ccggttctgt cataggaaga agccaggttc
    13981 ccctcctttc aaagcaaata catgatgaaa taaaggttga tctttcatat cacaacattt
    14041 cagatttagt attatgcatc agcatgttcc ctaatgaagt tgcagtcggt ctcgccggca
    14101 tatcaggcag gagagaggaa tgaagagata cgacaaaggg aggaagctca tacttcagga
    14161 cggaccagac tctatggtgt ataagtcctt attgcgtcaa cctctgtccc tcccctggcg
    14221 atatcttccg gggttgtatt cgaaacactt atttcggtgc ccaggaaatc ccttcctgcc
    14281 tcttgatagc aatgtctcac aaaggagctg caatacatgg cgtgaggatc gttgaaggga
    14341 ttttccagcc attgcctcct tgcgattatc gcccaccatg tcccgaggag ttcctgaatt
    14401 ggatagagca cctgttcatc aacgagctgt aacgcagtgg cgaggacatc gtccttctcg
    14461 tcctctgaaa gcccaaaatc tatgagcgcg gcattctcga ctgtcgtacc gcaccacttt
    14521 ccgatccagt tctcctgggc cccatttctc agttggggtt taaaaagctt tatcttgagg
    14581 tcgctctcaa aaatataggg gctctcactc ctggcatttc tcggtcccct tcggtcaagc
    14641 cttaattcac caagtataaa ggaatgagac cagagggatg gtttcttgtc ccctgtcact
    14701 gccctctggg cctctcttac ggcctgacca atgggatcat tgctccccac aagccctatt
    14761 gtcccgggct tatggtgctc ggtgaaaaag ttgagcaatc tttcattcac gtcttccacg
    14821 ggtcttcacc cccttcgtgc tccaaaattc aggattccgg agtaaaacgg cgatccttcc
    14881 cttaggcaca aaaataatgg ggttgccgaa tccgctggag gaggttgagg tggaaataga
    14941 gcggattcgg caacaatctt ttcactccca aaataagtat ctcagtgtca gggaggaatt
    15001 tctacacatt ttttgctttt ttttatgcat tttttgcaca tcataaagat atgatgatct
    15061 aaggtcataa tgtgaggaaa tgagtcagga tagtgcctaa acgtctaata cgttattact
    15121 caggaggaat gccctgcgag aagggttact ttactcagcg gcattggaaa tggctattgt
    15181 gcctgctcaa gacctttttt tgccttggca ttcgccgggt ccaacttaag ggccttttca
    15241 aattggagtt ctgccttttt cttatgtcct cccttaaggt agaggatacc aaggtttgca
    15301 aagtgttctc cactgtaagg ttcaagcttg atgctctcga ggagcgcctc ctcggcatcc
    15361 ttcatcctgt gcggcatctt gctcagggcg agagaaaggt aactccacgc ctttggattc
    15421 ctcggattat tctttgtcac ccatttgaat atctcgacag ctccccagaa gttgccgttt
    15481 ttgaattctt caactcccct cctgaactgt tcctcgatga gtactgtttc ggtatcctcc
    15541 tcttttttcg gtattttttc caaggcacca aaaaactcca ttctcctttt ttcgtctttc
    15601 agtgagttgt atgcttgggt gatggtatca aagataacgg tgactttagt tcttaaggtt
    15661 gcatcgcctg aagcatgcac cctgtcggga ttaaattcct ttgtcatcct ttggtatgtc
    15721 ttctttatct cctcagggcc tgttttttca ttggcctgga gcatttcggc gggactcatg
    15781 cgcggcagtt ttgcatacag ttcctctacc ttgttcttta aattcttttc ttcctcagcc
    15841 atggatacga ggatttcttc aacattaatg gtttcctgca cgtcgagacc atgttcagca
    15901 gtttcctttt gcgtaatgaa ccctgttgac cagaggacat agagagttct catcacatca
    15961 aaagaattca tgcgtgactg ttctattacc tgatttatgc tcctcgttcc gtccacaagg
    16021 gaaaggattt tcgcgtcctg tggactcagg tcgattcccc tgaagattcc tgaggggtct
    16081 tcattgaggg taaatatcgt atgggcatcc ggcatgtccc tcctgatcct tgtcaaatta
    16141 ttgatcttgt ttacgccctc gtaaatgagg ttattcgtac ttatcttgag tgtgatcacc
    16201 tcatcgggag gaatatcctc aacgaactcg tactccccct cgtcaagttg aaagaggctg
    16261 tagatgatct cctttacctg gtacttgaca ccccagaaga gatccttagg tgtgatatat
    16321 cccagttcca cgagaatagc tccctgacgc ttgcccgttg atttcagaag ctcgacggat
    16381 ttgtcatatt gccccatggt tatcttgccc gccttaatga gcatctcccc aagcctgtcg
    16441 tcctcgaagg tggaggaggc aaatatcaca tttccccccc ggatgtagat tttttttgtg
    16501 aaacgggggg tggttatgga aagggtccct gtttccctgt ttctgttgag ctcaatgagt
    16561 atctttggca ggctgtaatc cctgagatat cctctgaaag gcatgatcat ttaatggtat
    16621 ctcctcacct cgaaccgata gatatcggcg ctttcggacg gatctatccc tgccttcatt
    16681 tttgcaatcc tcacctgttc ctccactgtt tccaccccct ccaaatcagg gaggaggaga
    16741 cccctcatgc cccccttgac cacaatgacc ccgtattttt taggatccag ttcctttggg
    16801 tcagagattt tttctggtgg cgagaggaca tcgacagaat aggtaataaa agggagttcc
    16861 tcttttgcga ccagagggaa acggggatcc tctgttgcag cgcaaatcgc attctttatg
    16921 gtctcttccg caacattccc gcaacatggc aaaaaagtgc cgatgcaccc tctcaattgg
    16981 ccctcttttt tcagacagac aaaaaccccg gccttttcgg acatcacggg agtgaggggg
    17041 tcagggggtt ggatacccct ttttttaagg atcaattcct ccactgccct ctttgcaagt
    17101 tcgaccaggg gatccattat ctgtccctta caagtgcgtg gtcagacatc gcccgcaggg
    17161 catccctctc cggtgaaggg gcgaatattt caagctcact ttttgcctct gaaacaaggc
    17221 cctgtgcaat tctgagggat tcatctatta cgcgatattt tccaaacagg tgaagaattc
    17281 tttttaactc tttttctccc gataccccat tcttagtgat accctttatc tctgcttttt
    17341 cttccccaga ggccacaccc aggaggtaga taagggggag ggtaattttg ccttcaagga
    17401 ggtctttccc gagttttttc ccaagccctt tctcatctgc catataatcg aggatatcat
    17461 ccaccatctg aaatacaatg cccgtcttca tgccaaagcg gccaagggca tcctcttttt
    17521 cgggagaaag ccctcccaga atgccgccta tcctgcatgc tgcagaaata agggcccccg
    17581 tttttgcgga tatgatcctg tagtactctt cctcagtgat atcggggtct gcaactctgg
    17641 tgagctgaag gatctcaccc tcggtcattc tggttgtcgc ctcggaaagg gcctccatga
    17701 tcctttggtt cttctggagt accgcgagtc tcagggcatt ggaatagagg aaatctccca
    17761 cgagaatcac tatctggttg ccccatatca tgtgccccgt ctgcttgccc ctcctcaatt
    17821 ctgcgctgtc caccacatca tcatggagga gcgatgcggt gtggatggcc tcgataatac
    17881 tcgcaaggac agtccttttt tcacctttgt aacgggccag atcagcactc aggaggagga
    17941 agaggggcct gagccttttg ccgccgccac ccaggatatg ttccccgatc accggaagga
    18001 gagaagcatc gctggtgaat atggtcctca agttcttttc cacaaggccc agctcctctg
    18061 tgtagagtga aaaagtatcc gtaaacatta gtggcgatcc atcgtcttaa tatcttcagg
    18121 cctgaaaatg cctttttctg taattatagc agttatatac ctggccgggg taacatcaaa
    18181 tgccatattc ctcaccgcaa caccctcagg ggcaatccgg acagctcccc aggatacacg
    18241 ggggacgttc ttcccttttt gtcccctctc attctcgcag gattcatcct ctgcactgcc
    18301 ctcacaatac gaaattcttc cgtagatatg ggtcacctca gaagggtcac gctcctcaat
    18361 aggtatttcg tctcccgtgg ggatcgacag gtcgatactg ctggtgggcg ctgccacata
    18421 aaagggtatg ttgtgctcct ttgcgagcac tgccaggggg tatgtcccta tcttgttcgc
    18481 cacgtcacca ttcctcacgg tccggtccgt accgacgata ataaggtcta ttttgccttt
    18541 cctcatgagc gaacctgcgg agttgtcagt aatgagagtt accgggatac cttcctgcat
    18601 cagctcccac gcagtgagtc ttgcaccctg gagcaccggc ctcgtctcgt ccgcaactac
    18661 ctgcatcctt ttcccttgct ccttcgcata cagcataggg gcagtggccg tgccgtatcc
    18721 cccggttgcc agagcgcctg cattacagtg tgtgagaatg gtatccccgt cctttatcag
    18781 ctctgctcca aacctcccga tcgacctatt cacctcgata tcctcctgga gtatcctttt
    18841 cgcctcttcc acaagcagtt ccttgagcgc aggaacgggg cggtcctttt ctcttgtgag
    18901 catctccttt atccgtttca cggcccattg tatattcact gcggtgggcc gtgtggcaag
    18961 catggcactg aacacaggtt cgagcatctg catgaagtgg tcatacgtct ctgcattgag
    19021 ggcctttgct cccagtgcaa tgcccatggc cgctgcaatc cctatggcgg gcgcccccct
    19081 tatttggagt tccctgatac cctctgcaac cattgcataa tccgtgcact caataaaccg
    19141 cacctctgag ggaagcctgc tctgatcgag cattatgacc ttatcatctc tccattctat
    19201 tgtcttaacc atctaagcct ttcctcccag ggggaatatg atggtgagaa tcagcagaag
    19261 agtcaaacag ataatcacgg ccactgttcc ccacgctata taattatagc tctttggatt
    19321 ggcatatttc cccataagtt ctttattgtt tataagggac agggcataaa tcaagacaaa
    19381 gggaagaagc aatccattta ccaaagagga aagtaccatc aaaaggacga gaggggcctc
    19441 tggtatcaag atgagcaggg ctgacagtac gatcatcagg gtatatatcc acataaactg
    19501 tggagcctcc ttgaagttct tgttgacccc tgcctcccag cccatggctt cacagatata
    19561 gtacgctgtt gaaataggta ctatgattgc accgagcaga gaggcattgg cgagactcag
    19621 ggcaaatatg agagatgcat aatcccctgc aaggggtttc agcgctattg cagcctctgt
    19681 cgcttcattt attcttattc catggggaaa caaaagagcg ccgcacgtca caattatgaa
    19741 aaagctgata atgtcagtta ttaaacatcc ggtaatcaca tcgagccttg agaccttata
    19801 gtcctcgggt gttatcccct tctccacaat ggaagactgg agatagaatt gcatccacgg
    19861 ggttatggta gttcctatga tagcgatgct cagcatgata tattccgaat tccattgcaa
    19921 tcgtggtgaa aaggtgtttt tcataatgcc accccagtcc ggcttggcca tgacccctga
    19981 gacaatatac cctatataca caagacatgc gaccaggagc accccttcca caaaccggta
    20041 acttcccttg gtaaccagta tccaaataga tatggcacct aagggtacca taatgtattt
    20101 gcttaatccc aggatctcca tgctggctgc ccagcccgca aaatcggcaa cagtagttcc
    20161 aaagttcgcg acaaggagac caaccatcat gaaaaaagct gttttgaccc cgtagctttc
    20221 acgaataagg taggagagcc ctttccctgt taccacaccc agacgggcaa tcatttcttg
    20281 tatcacgaca agcgcgaccg tggtgggaag gagggtccag agaagtccgt aaccaaagcg
    20341 tgcgcctgca acagagtaag tggtgatgcc gcttgcgtca ttgtcgatgt tggcagtgat
    20401 aatgccgggc cctatgatcg atgcaaacat cactattttt tgccaacgcc tcatttccct
    20461 ctcctgccgc cataagcctt aaaatgaaga gaatcaacga ttatccaggg ttactattat
    20521 atccaatggt gcgtattttt tcttgtggag aaagggcttg ttatggtaat attaaaaaat
    20581 ttccggaggt gtataaatgc gaagactgtt gttctctgtg gtaatggccc tcatgctctt
    20641 gcctgtgtcg tattcttatg gcttcagcga aaaaggccag gattgttcaa aatgtcatac
    20701 cttaactaag gatgaagccg ccaccctgat gaaaagtttt ggacaggagg tgaaggtcct
    20761 tgaggtcctc acgagtccta ttaaaggaat gtgggaagta gatgttgatg ccggtggtaa
    20821 gaagggattg gtgtatattg atttttccaa gaattacctt gtatcgggtt cgattatctc
    20881 actcaagaac aagacaaacc tcagccagga aaggttcacc gagctcagca aggtggatgt
    20941 ctcgcagatt ccccttgatg acgccattgt gatgggtgaa aaagatgcaa agtacaagat
    21001 tgtggtgttt agcgaccccg attgaccata ctgcgggaaa cttcatcagg agatgaagaa
    21061 ggtcatagaa gagaggaaag atattgcgtt ctatgttaag atgtttccgc ttccgatgca
    21121 taagggtgcg gatatgaagg caaaggccat agtctgtgaa aagtctctcg ctctccttga
    21181 ggcagcgtat gagcataaag agttgcccaa gccaagctgc gagaccactg ttattgatga
    21241 gaacatcaag cttgcgcaaa aactcgggat ttcgggcacc cctgcaatga tttttcctga
    21301 cggcaaggta gtgccgggcg ctattgatgc caaggccata atagaaaaga tcgggaaata
    21361 gcaggcatat cctgtcagag actatttgtg atgtgcatag gggtttgact taataaaccc
    21421 ctatgatttt tctgccgaag tggcggaact ggcagacgcg ccaggttcag ggtctggtgg
    21481 tcgcaaggcc gtgcgggttc gagtcccgcc ttcggcatta tttatttctt tttaaatcaa
    21541 taggttagta tctaagatgc agcaaaaaaa ctgtccccag actgtcccca gcaggctctt
    21601 ctataattct ttagtcacgt ttttaaaaac accacctgac aaggaatccc ttccttttca
    21661 aatgccttct ttagctcctc tcctttttga gaaaagaagc tgtcttccat ggcccactct
    21721 aacccatatc ttttatacgc ccaatctgcg taatggtagt tttgttgaga attactggtt
    21781 catatgcgtg gcgctattgc cacttatcgc aaacaaaatt gtgatcttcc ttctgttata
    21841 attgtttagc attctatcct cggaggggtc tgtggatgtt ctaataatag actgtacaca
    21901 gcaccatgca aaggcagtca agtccatcgc atatcactta aatcgttggc aaaacgaatt
    21961 tcgttttcgg gcagtaagag tgtctcacaa acacgattct ctcaaatgcg ttatccagag
    22021 atgttgcaac aactttcgta aaacaatgtt accaagaaca gtcatccttt tccttcacaa
    22081 tcggcatcat agataatcca tttgtagata actggttcag ttacacggac cataaaaata
    22141 agtgttgtct cataacaaca gatagttgga acgttctctc ttatctgcca ttggagtcct
    22201 ttttgggtat tgagattatc cagaacattc aggaatttct tatggactgg caagattttt
    22261 cttatgctta tccggacccc ctcggttgca tcaatgatct ctgcgggtac aagccggata
    22321 tctcttttaa aatcagaact ggcgacgatc ttttatagaa actgccagaa aaccccgagc
    22381 tttcaagctc ggggatgaat ggctgcctgg agcgaagcca gtgaaagctg gcggagcgga
    22441 ggtatctttt ggcataatct ggccatggcc gaatattgga caggcgcaca cacgaagcat
    22501 cgtttgcagg tgcacctggt gtggataccg aagtaccgaa agcgggtgtt aagaggcaaa
    22561 atcgccatac ggttgagaca attgttgcgt gaagcgtgca gaatgaatcg gtggcacata
    22621 agcgaaatga gtttacaaac tgatcatatc catctgatca tgcagataaa gccgagcgaa
    22681 agcatatcag gagtcgtaca aatattgaaa ggtggtacga gtcgggcact aaggaaagaa
    22741 tttcctggat tggaggaatt tttgtggggg gatagttttt gggcagatgg ctattttgcc
    22801 gagagcgtag gtcacgtcga cgaagatatt gtgaaaagat atatcaggga acagcgggga
    22861 aggtcatgag ccggattatg ccagaggtca gaaactccgg gctttagccc ggagagtagg
    22921 tcatttggga tatcttctgt gagggacctg atagggattg gggcgctttg ttcagctcca
    22981 gttccagttt gctggcctcg atggcaagca ctgccagcct ctgcgtcagg tcatcgtgga
    23041 gctcacgtga cagacggcgc agttcctctt cctgcgcaga gatcagtctg cctgcaagca
    23101 tctggagatt tttctgactg tatcttagag cggtctcgct ttcccttagt gcttcttcag
    23161 ccatcttgcg ctcggtga
//
LOCUS       DATKOU010000124        11075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_934189, whole genome shotgun sequence.
ACCESSION   DATKOU010000124 DATKOU010000000
VERSION     DATKOU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934189"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            541..1398
                     /locus_tag="VMU21_05775"
     CDS             541..1398
                     /locus_tag="VMU21_05775"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HUO77069.1"
                     /translation="MLIIGEKLSIIAKRVREAMMKKDKGPIQEIAARQWKEGSGVIDA
                     NIGPAEDGGEDLMQWMVTTIQEVVPLPVCLDTTNFKAIEAGLKVHNNEWGRPFINSTS
                     NDPERFPILELGAKYSANVIGLTVGKGGLPADAEERAAIAAEIMGRAMEYGVPLEDLF
                     LDPLVLQIATSQDHARHVIKAIAMFQEMNDPPMKTVVGLSNISNGCPKTLRPILNKYY
                     FLLLADAGLTAAIADAHEMAEAVHDKALVADVLAGKQLSDPKKMAEISKTLDVIMGRT
                     LYAHSYLEM"
     gene            1487..2425
                     /locus_tag="VMU21_05780"
     CDS             1487..2425
                     /locus_tag="VMU21_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HUO77070.1"
                     /translation="MAFTAPTETYPGKVFEVTIGTDKAANFGGENVLSFHSFEGNVPN
                     KPVLAYEIQDIPPDDWPETVRKVYDGVSGDPVTWAKHCQDKLNAKAIALRLIGTHPDR
                     ENRSPEDAAKTVKDVLAAINVPFIILGSNNAEKDAMVLVAVAEVAKDKNCIIGKAQEA
                     NYKTIAAAAMANNHKLIAMSELDINLSKQLNILITQMGFEKERLITDPMCSALGYGLE
                     YTYSVMERIRLAALTQNDTTMQPPMLGDVGMYVWKLKETQAPESDLPQWGSLEQRGVA
                     WETLTAGGLIIAGAELLIMRHPDAVRAVEKFIDELM"
     gene            2439..3779
                     /gene="acsC"
                     /locus_tag="VMU21_05785"
     CDS             2439..3779
                     /gene="acsC"
                     /locus_tag="VMU21_05785"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HUO77071.1"
                     /translation="MALTGVEIFKLLPKTNCKKCGHPTCLAFAMKLAQRQASLDACPD
                     VSEEAKRVLGEASAPPVRPITLGVGDKAVKMGEETVLFRHEKKFVNPCAFAVEIKDTM
                     SDGDINRIAEEVVNSEIDRVGQKLRVDAICISNASNDAGKLVAAVKTVAAKAPAVPLI
                     ISTGNPAAAEAAVKVVADKRPILFGADESNADAMANVAKANKAILGVSAKGLDALSSL
                     TEKIKGLGVEDMVIDGGARSAKEILRDNTLVRRAAIKKNFKPLGYPVITFAQRDDSML
                     EALVASLGIAKYASIVVVNSVEKWKNLALFTLRQNIYTDPQVPMQVEQKIYKLGEPTP
                     ESPLMITTNFSLTYFIVSGEAENSKVPCRLAVMDSEGLSVLTAWAAGKFTASKIATFI
                     QESGIENEIKHRELIIPGYVAILSGSIEEKLPGWKVTVGPREANALPAFLRSRA"
     gene            3878..6076
                     /gene="acsB"
                     /locus_tag="VMU21_05790"
     CDS             3878..6076
                     /gene="acsB"
                     /locus_tag="VMU21_05790"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF007078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HUO77072.1"
                     /translation="MSKLIASAAIRGAQKLVKQAEEMLEKVIAEKGKDFKFEFPDTAF
                     YLPMIYAMTAFPVKTLGDMRVGLGFAKELLHEEPKEHVWKPYLGEALDSGMATLFAEE
                     IILAIRYIHGLEPCKDPETGYVYNGFISDTIQRNLGIQLVDGTMPGFAAIIGAAPTDD
                     IAVNIVRELQEKNILTFLSGQSNGNSVTKQLLKKGVELGWDTRIVPLGPDTEHTLYAL
                     DWAIRASLIFGGKKPGAFKEHLMYQKDRVFAFAIVLGPLDDIKWTTGAGAINMGFPAV
                     GDTDIPVIHPTGVCTYEEVDKELDHSKIVQKAIEMRGLKITVEKPPIPVAYGPAFEGE
                     RIRKEDTFIEFGGQRTAAFEWVRMRENDEIEDNKIIIVGDKWQERYEKGGQLPLGILI
                     EASGRKMQKDFESVIERKIHSNINEAQGVWHMGQRDINWVRISNAAKKEGFTLEHLGI
                     LNATMTHHRFRSIVDKVQVTLFIDEADVKKMMDEARMAYRERDLRLGSMTDEAVDTFY
                     SCLLCQSFAPSHVCVITPERLGLCGAYNWLDGKAAYEIDPTGGNQPIAKGECLDSRYG
                     RYTGSDEYLKKATGGAVETLNLYTIMENPMTSCGCFECIVAIIPEANGVMIVHRGHTG
                     MTPIGMKFSTLAGTVGGGAQTPGFMGIGVNFISSKKFLFGDGGIKRIVWMPKGLKERI
                     AEDFKKTSEAAGVPGLLDKIADESICEDSEKLLEYLQKVGHPALEMEPMI"
     gene            6129..8135
                     /gene="cooS"
                     /locus_tag="VMU21_05795"
     CDS             6129..8135
                     /gene="cooS"
                     /locus_tag="VMU21_05795"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HUO77073.1"
                     /translation="MSEEAKKEAHLKSANPNSEKVHEWGVEQKMDTVFERAQKMKPCP
                     IGHVGACCKHCHMGPCRLVGKNAEEEITGVCGASLATVTARNFLRMIAAGTAAHSDHA
                     RGMAEALLAVAKGETKDFKVTDVRKLYKVAGILEIEFEGRPVNDVAKDVATTLLNDFG
                     RQHGGELNYVKRAPKKTQERWRKWGIVPRSIDREVVETMHRTNIGVDHDPDHLLLQGL
                     RTALADGWGGCMISTDITDILFGTPNPQQAKMSLGIFKEDEVNFIVHGHEPLLAELIV
                     ELASDPEMIAYAKSKGAKGINLGGMCCTANEILVRHGIASAGGFTNQELGIMSGLVDA
                     ISVDVQCIMPAIVELSKKFHTKVITTNPKAKIPGAMHIQFDEHRGKEIAKEVLKVAID
                     NFPNRKAKGSRITEVFPAIAGFSHEYIEYMQGGKWRGSFRPLNDAIMAGRVRGVVGLA
                     GCDNPRVPSTGLHRFLATELIKNDILIVSTGCGSAACGTMGYLTPEMAMENAGPGLRE
                     VCEAIGIPPILHLGSCVDNSRILTIASAMAAEGGLSDEIGGMPAVGIAPEWMSEKAIA
                     IGCYFAASGVPVIFGGESPVAASKEVTKLMTEGWFERFKGALHFEPDQEKILAMAIDY
                     IDKARAALKLKKYERGKFATEKTLMDMAARREIEKAAKPHMGIY"
     gene            8343..9155
                     /locus_tag="VMU21_05800"
     CDS             8343..9155
                     /locus_tag="VMU21_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUO77074.1"
                     /translation="MVFLVISGVVLVYVLLIAYVYVKQGSMLYFPLKEIEATPLAIGL
                     DYQELILRTKDGVDISAWYTPAENARGYVLFCHGNAGNISHRLDSIRIFHNLGLGVLI
                     FDYRGYGRSKGAPDEEGTYRDAEAAWDYLVNSLHVAPEKIILFGRSLGSAVAAEMASR
                     KQAGALIMESGFTSVPDLGSSFYPYLPVRLLSKYRYASIEKVGRLKIPKLFIHSPEDE
                     IVPYEQGRKLFESASEPKEFLQLSGGHNEGFLLSGETYLKGLDSFFSRYLPD"
     gene            complement(9381..10829)
                     /gene="gltA"
                     /locus_tag="VMU21_05805"
     CDS             complement(9381..10829)
                     /gene="gltA"
                     /locus_tag="VMU21_05805"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HUO77075.1"
                     /translation="MAELTQKERMKIKRHEMSNQDQHVRRGNYDEVALGYSLETAREE
                     AERCLQCKDPKCVQGCPVGVLIPQFIKALREGDIVKAVEWMKVKNNLPAICGRVCPQE
                     VQCEGNCIVGKKGEPVAIGRLERFVGDYELAMRTCPLLKAPKTGKRVAVVGSGPAGLT
                     CAVDVAREGHEVTIFESLHGPGGVLIYGIPEFRLPKSVVHGEINYAVECLGIDIKVDH
                     VIGRTFTLEELLKEYDAVFLGTGAGLPSFMRIPGINLNGVMSANEFLTRVNLMKGYRF
                     PEYDTPVKVGNKVAVVGAGNVAMDSVRCSLRLQALQAREAGQNSGEVHLVYRRSRDEV
                     PAREEEFHHAEEEGVIFDFLTNPVEILGDKEGNVRGIRCVRMRLDEPDQSGRRKPVPI
                     EGSEFEITVDSVIMALGTSPNPLVFVDAAGLERTKHGTAVADSETGRTKKPRVWAGGD
                     VVTGAATVISAMGAGKRSAADINKFLKGEASW"
     gene            complement(10829..>11075)
                     /locus_tag="VMU21_05810"
     CDS             complement(10829..>11075)
                     /locus_tag="VMU21_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77076.1"
                     /translation="PTVVSLNTIMIDGTGMCGGCRVLLDEGAQFVCVDGPEFDAHKVN
                     WESLLSRLQFYKKEERLAVERWEHKCKLEGELPGGGK"
BASE COUNT         2792 a         2767 c         3009 g         2507 t
ORIGIN      
        1 ttccttctca tctttttctt tctctgcctt ggcgttcaga ctgaaaatgg cgtcagttcc
       61 tgtctgaggc tcttcagggg aaccttcagg gatgccacca aaatccttca cttccatcac
      121 ctcctctttc ttataatcga agttatcatc gatttgaaca cataaaagca gattccatgc
      181 cagcaccctg acctttgaaa tccattgatt attaaataga attaatttcg ctctcattgg
      241 ttacgaagag atatgacaaa atttgtcatt agagaggcca taaaatgtcg gcaattctca
      301 tctgaggtgt ttctgaccgt cccattcatt tcagatattt tagattttgg catcaggatt
      361 gtagcttcct tctctttgca ttttttacta taaaaatcac aaaaaaagac ttgacaaccc
      421 atggctcctg gaacataata gttcgttata atttgcataa ttttgttaaa tagatagtcc
      481 tgcgcaaaaa aaattagcaa tagacagagt aacaccatta atgaaaggag tcttcgggag
      541 atgctaataa tcggcgagaa actgagcatt atcgcaaaac gtgtgagaga agcaatgatg
      601 aagaaggaca agggtcccat ccaggagata gcagcaaggc agtggaaaga agggtcgggt
      661 gtgatcgatg ccaacatagg ccctgctgaa gacggaggcg aggaccttat gcagtggatg
      721 gtcaccacca tccaggaggt cgttcccctg cctgtctgtc ttgacacaac gaatttcaag
      781 gctatcgagg cagggctgaa ggtgcataat aatgaatggg gcaggccgtt catcaattcc
      841 acctcaaacg accctgagag attccctatc cttgagctcg gggcaaaata tagcgcaaac
      901 gttatcggtc tcactgtggg aaagggcggc cttccggctg atgctgaaga gagggctgca
      961 atcgcggcgg agattatggg acgggcaatg gaatacggcg ttcctcttga agacctcttt
     1021 cttgatcctc tcgtactcca aattgccaca tcacaggacc acgcgcgcca tgtcattaaa
     1081 gcaatcgcca tgttccagga gatgaacgat cctcccatga aaacagtcgt cggtctcagc
     1141 aatatatcca acgggtgccc caagacgctc aggccgatcc tgaacaaata ttatttctta
     1201 ctattggctg acgccggcct gactgctgcc attgccgatg cccatgagat ggcggaggct
     1261 gtgcatgata aggcccttgt ggctgatgtc ttggccggaa agcagctctc tgaccccaag
     1321 aaaatggcgg agataagcaa gacacttgat gtgataatgg gcaggaccct ttacgcgcac
     1381 tcatatttgg aaatgtaaaa tcagctgtta gctcttggct catagctcat gatcacgaca
     1441 atgagctatc tgccatgaac tatgagcgtt aaattggagg tataacatgg ctttcaccgc
     1501 accaacagag acgtatcccg gaaaagtatt tgaggtcacc attgggacag acaaggcagc
     1561 gaactttggg ggagaaaatg tcctctcctt ccattccttt gagggaaatg tcccgaacaa
     1621 accggtactg gcctacgaga ttcaggacat tccccctgat gattggcccg agacagtacg
     1681 aaaggtctat gacggggttt ccggtgaccc ggtaacctgg gcaaaacatt gtcaggacaa
     1741 gctgaacgca aaggcaatag ccctcaggct catcggaacc caccctgaca gggaaaaccg
     1801 ctcccccgag gatgcggcaa agacagtgaa agatgtactc gccgcgatca atgtaccctt
     1861 catcattctc ggcagcaaca atgcagagaa ggacgccatg gtgcttgttg cggtggcaga
     1921 ggtcgcgaaa gacaagaact gcattatagg gaaggcacag gaggcgaact acaagaccat
     1981 tgcagcagcg gcaatggcga acaaccataa gctcatcgcc atgtcggagc tcgatataaa
     2041 cctttccaag cagctcaata tccttattac ccagatgggc tttgaaaagg agaggctgat
     2101 tactgacccc atgtgttccg ctctcggtta cggactggag tatacctatt cggtcatgga
     2161 aaggataagg ctcgctgcgc tcacccagaa cgatactacc atgcagcccc ctatgctggg
     2221 cgatgtggga atgtatgtct ggaaactgaa ggagacgcag gcccctgaat cggacctccc
     2281 ccagtggggg tccctggagc agaggggggt cgcatgggag accttgaccg ccggcggcct
     2341 cattatcgcg ggtgcggagc ttctcatcat gaggcatccg gacgcggtga gggccgtgga
     2401 aaaattcata gatgagttaa tgtaagggag gaatagatat ggcattgaca ggcgtagaaa
     2461 tattcaaact gcttccaaaa accaattgca agaagtgtgg gcatcccaca tgccttgcct
     2521 ttgcaatgaa acttgcacag aggcaggcat cccttgatgc atgtccagat gtctcggaag
     2581 aggcgaagag ggtacttggc gaggcatcag ctcctcctgt tcggcccatt acccttggtg
     2641 tgggcgacaa ggcagtaaag atgggcgagg agacggtact tttcaggcac gaaaagaagt
     2701 ttgtgaaccc gtgcgcattt gcggtagaga taaaagacac gatgtctgat ggagacataa
     2761 acaggatcgc tgaagaggtg gtgaattctg agatagacag ggttggccag aaattgaggg
     2821 tagatgccat ttgcataagc aatgcgtcaa atgatgcggg aaaacttgtg gctgcggtga
     2881 agacagtggc agcaaaggcc cctgcagttc ccctgatcat ttccacgggc aatcctgctg
     2941 ctgccgaggc tgcggtcaag gttgtggctg acaagaggcc gatccttttt ggtgcggatg
     3001 agtccaatgc agatgccatg gcaaatgttg caaaggccaa taaggcaatc ctgggggtaa
     3061 gcgccaaggg acttgacgcc ctttcctcac tcaccgagaa gataaagggt ctcggcgtgg
     3121 aggacatggt cattgacgga ggcgcaagga gcgccaagga aatcctccgg gataacaccc
     3181 ttgtgagaag ggctgcgata aagaaaaact tcaagccgtt gggctacccg gtcatcacgt
     3241 ttgcgcagag ggatgacagt atgctcgaag cgcttgtagc aagcctggga atagcgaaat
     3301 acgcttccat tgtggtggtc aacagcgtcg agaagtggaa gaaccttgcg ctctttaccc
     3361 tgaggcagaa tatttacaca gatccccagg tgcccatgca ggtggaacag aaaatataca
     3421 aacttggaga gcctactccc gaatcccctc tcatgatcac cacgaacttc tctctcacct
     3481 actttatcgt ttcgggcgaa gccgagaaca gcaaggttcc gtgcagactt gcggttatgg
     3541 acagtgaagg cctgtcggtc ttgactgcgt gggctgcggg aaaatttacc gcatcgaaga
     3601 tcgcaacatt catccaggaa agcggcatag agaacgagat caaacacagg gaattgatca
     3661 ttccgggcta cgttgctatc ctgagcggtt ctattgagga gaagcttccc ggctggaagg
     3721 tcactgtggg accgagagag gcgaatgcac tccctgcatt cctgagatcg agggcataga
     3781 aagccctgca gaaatatctg tgcagtgaga aataattaat ttgatttata caatataaat
     3841 tgtataaaat aaactactac gaaatgagga ggtcaggatg tctaagctaa tcgcatctgc
     3901 agccataaga ggtgcgcaga aactggtgaa acaggccgaa gagatgctcg aaaaagtcat
     3961 agctgagaag ggaaaggatt tcaagtttga gttcccggat accgcgtttt atctgcctat
     4021 gatatacgcg atgaccgcat ttcctgtaaa gaccctcgga gatatgaggg ttggcctcgg
     4081 ttttgcgaag gagcttctgc atgaggaacc aaaggaacac gtctggaagc cctaccttgg
     4141 agaggccctt gattcaggga tggccacact gtttgcggag gagatcatcc ttgccatcag
     4201 gtacatccat gggcttgagc cgtgcaagga cccggagacg ggatatgtgt ataacggttt
     4261 tatctctgat accatacaga ggaacctcgg catccagctt gtggacggca ccatgccggg
     4321 gtttgctgcc atcatcggtg cggctcccac agacgacatc gcggtgaaca ttgtgaggga
     4381 gctccaggaa aagaatatcc tgaccttcct ttccggacag tccaatggca actcggttac
     4441 gaagcagctc ctgaaaaagg gcgttgaact gggatgggat acaaggattg tccccctggg
     4501 ccctgatacc gagcataccc tttatgccct tgactgggca atcagggcgt ccctcatatt
     4561 tggcggcaag aaacccggag ccttcaagga gcacctgatg taccagaaag acagggtctt
     4621 tgcttttgcc atagtgctgg gtccccttga tgacatcaaa tggaccacag gggcaggtgc
     4681 gattaacatg ggattccctg cagtgggcga tacggatatc cctgttatcc atcccaccgg
     4741 tgtgtgcacg tatgaagaag ttgataaaga gcttgaccac tccaagatag tgcagaaggc
     4801 gatcgagatg agaggactca agattaccgt cgagaaaccc cccatccccg ttgcctacgg
     4861 ccctgccttt gaaggtgaga ggataaggaa ggaagacacc ttcatagagt tcggcggcca
     4921 gagaactgcg gccttcgaat gggtgaggat gagggagaat gatgagatcg aagataacaa
     4981 gatcatcatt gtcggcgata agtggcagga gcggtatgaa aagggcggac agctccccct
     5041 tggtatcctc attgaggctt cggggagaaa gatgcagaag gactttgagt cggtcataga
     5101 aaggaagatc cattccaata tcaatgaggc ccagggcgtc tggcacatgg ggcagcgcga
     5161 catcaactgg gtgaggatca gcaacgctgc aaagaaggag ggatttaccc ttgaacatct
     5221 tggcatcctg aatgccacca tgacccatca ccgcttcagg tccattgtgg acaaggtgca
     5281 ggtgaccctc tttatcgatg aggctgacgt gaagaagatg atggatgagg cacgcatggc
     5341 atacagggag cgtgacctga ggcttggcag catgactgac gaagcggtgg atactttcta
     5401 ctcatgcctt ctttgccagt cctttgcgcc aagccatgtc tgtgtgatca cccctgagag
     5461 gctcggcctg tgcggtgcat acaactggct ggacggcaag gctgcatacg aaatagatcc
     5521 caccggcgga aaccagccca tcgccaaggg agaatgtctc gattcgcggt atggccgcta
     5581 taccggttcc gacgagtacc tgaagaaggc gaccggtggc gcagtggaga cactgaacct
     5641 ttacaccatc atggaaaatc ccatgacctc ctgcgggtgc ttcgagtgca tcgtggccat
     5701 tattcctgag gcgaacgggg ttatgatagt gcacagaggg cacacaggga tgacgcccat
     5761 tggtatgaaa ttctccaccc ttgcagggac cgtgggcggc ggggctcaga cccctgggtt
     5821 tatgggtata ggtgtgaatt tcatatcgag caagaagttc ctctttggtg acggcggaat
     5881 caagaggatt gtctggatgc cgaaggggct gaaagaaagg attgcggagg atttcaagaa
     5941 gacgtcagag gctgcgggag ttcctggtct cctggacaag attgctgacg agtccatctg
     6001 tgaggattca gagaagctcc ttgagtactt gcagaaagtt ggccatccag cgcttgaaat
     6061 ggagccgatg atataaagac cgaacacgtt accattatgc gtaacgtata taaagattgg
     6121 aggcgaacat gagtgaagaa gcaaaaaaag aagcacatct gaagagtgca aacccgaatt
     6181 ccgagaaggt tcacgaatgg ggtgttgagc agaagatgga taccgttttt gagcgtgccc
     6241 agaagatgaa gccctgtccc atcggccatg tgggcgcatg ctgtaagcat tgccacatgg
     6301 gcccctgcag gcttgtggga aagaatgccg aggaagagat tactggtgta tgcggtgcat
     6361 cattggcgac ggtgaccgcc cgcaatttcc tccgcatgat cgcagcaggg acagctgccc
     6421 actctgacca tgcccgcggc atggccgagg cgcttctcgc cgtggcaaaa ggcgagacaa
     6481 aggacttcaa agtaaccgat gtgaggaaac tttacaaggt ggccggcatc ctcgagatcg
     6541 agttcgaagg caggccggtg aatgacgttg ccaaagatgt ggcgactacg ctcctcaatg
     6601 actttggaag acagcacggt ggtgaactga attatgtgaa gagggctccg aagaagaccc
     6661 aggagcgctg gagaaagtgg ggaatcgtcc cccgcagcat tgaccgtgaa gttgtcgaga
     6721 caatgcaccg taccaacatt ggtgttgacc acgatcctga ccatctcctc ctccaaggat
     6781 tgagaaccgc ccttgctgac gggtggggag gctgtatgat ctctactgat atcaccgaca
     6841 tcctctttgg gaccccaaat cctcagcagg ccaagatgag cctcggcatc tttaaggaag
     6901 atgaggtgaa tttcattgtc catggccacg agcctctcct tgccgaattg atcgttgagt
     6961 tggcctctga ccctgagatg atcgcctatg caaagtcaaa gggcgccaag ggaatcaacc
     7021 ttggtggcat gtgctgcact gcaaacgaga tcctggtccg ccacgggatc gcttcagcag
     7081 gcgggtttac aaaccaggaa ctggggatca tgtccgggct cgtggacgcc atctcggtgg
     7141 atgtgcagtg catcatgcct gcgattgtag aactgtcgaa gaagttccac accaaggtta
     7201 tcaccactaa ccctaaagcg aagatcccag gagcaatgca tattcagttc gacgaacacc
     7261 ggggtaagga gatcgcgaag gaagtgttga aagtagccat cgataacttc ccgaaccgga
     7321 aggcaaaggg aagccgcatt acggaagtgt ttcctgccat cgcaggcttt tcccacgaat
     7381 acattgagta catgcagggc ggaaaatgga gaggttcttt cagaccactg aatgacgcta
     7441 tcatggcagg cagggtccgc ggtgttgtgg gactcgcggg atgcgacaac ccgcgcgtgc
     7501 cgtccacagg tctccacagg ttccttgcta ctgaactcat caagaacgac atcctgatcg
     7561 tctcgaccgg ctgcggatct gccgcatgcg ggactatggg ctatctcact cccgaaatgg
     7621 ccatggagaa tgcgggcccc gggttgaggg aggtctgcga ggcaatcggg attccgccga
     7681 tcctccatct cggttcctgt gtggataatt caagaatcct caccatcgcc agtgccatgg
     7741 cagcagaggg cggcctgtcc gatgaaatcg gcggcatgcc tgcagtgggt attgcccctg
     7801 agtggatgtc agaaaaggcc attgccattg gatgctactt tgcggcatct ggcgttccgg
     7861 taatcttcgg tggtgaatca ccggtcgctg cgagtaagga agtgacaaag cttatgaccg
     7921 agggctggtt cgagagattc aagggcgcat tgcactttga gccggatcag gagaagatcc
     7981 ttgccatggc catcgattat atcgacaagg ccagagcagc cctcaagttg aagaagtacg
     8041 agagagggaa gtttgctaca gagaagaccc tcatggatat ggcagcgcgc cgcgagatcg
     8101 agaaggctgc gaagccgcat atggggatat actaagaata gctccagagt atgacgaaag
     8161 ccctgcagcc ttggctgcag ggcttttttt tcgattgagg cgaatcaccc ttgctcttcc
     8221 gtaatgtctt gggggtgaaa tcatgcgagt gatatactga actcacaggg tgcgttccat
     8281 gcaatatcga ttagagaggc ttgaagggtt ttattagtgc tgtgctgagc atggtgaaaa
     8341 gaatggtatt tctcgttata agcggtgttg tccttgtcta tgtgctcctt atcgcgtatg
     8401 tctatgtgaa acagggtagt atgctctatt tccccttaaa ggaaatagaa gccactcccc
     8461 ttgccatcgg gcttgactat caggaactca tattacgaac aaaggatgga gtggacatct
     8521 ccgcgtggta tacccctgcg gaaaatgcgc gggggtatgt gcttttctgt cacggaaacg
     8581 cagggaatat ctcgcaccgt cttgactcta tcagaatatt tcacaacctg ggtcttggcg
     8641 tgctgatctt cgattatcgg ggatatggca ggagcaaagg tgctcctgat gaagaaggca
     8701 cctatcgtga tgcagaggca gcgtgggatt accttgtgaa ctcactccat gttgcacctg
     8761 aaaagatcat cctctttggc cgttctctgg gaagcgctgt tgcagccgag atggcatcac
     8821 ggaaacaggc gggcgcgctt atcatggagt cagggttcac ctcggtgcct gacctgggaa
     8881 gcagctttta tccctatctt cccgtgaggc tcctctcaaa ataccgctat gcgtcaattg
     8941 aaaaagttgg aagattaaag ataccgaagc tcttcatcca cagtccggag gatgagattg
     9001 ttccctatga acagggaaga aagctttttg agagcgcaag tgaacccaaa gagtttctcc
     9061 agcttagtgg cgggcataat gaaggattcc tgctttcagg ggagacatat ttaaaaggcc
     9121 tggatagttt cttctcccga tatcttccgg attaacagac acactcagcg ttttttttcc
     9181 tggcacactc atataggcgg catggaaaga gagtcgcgct ccggctttca gaggcatcgc
     9241 gagcctctac ctcgcgtacc gcgttggata cgaataccct tgcgacttta agaatcggga
     9301 gacggggttt gagcaaagaa gccttgcgtc attttgcgca aggcttcttt gctctcagct
     9361 cttgggagaa aagaactttg ttaccaggaa gcttctcctt tcaggaactt gttgatatct
     9421 gccgctgagc gtttgcccgc acccatagcg ctgatgactg tggcggcacc ggtaaccacg
     9481 tccccgcctg cccagacacg gggctttttc gtcctccctg tctcggagtc ggcaactgcg
     9541 gttccatgtt tggttctctc aagtcctgca gcgtccacga agaccagagg gttggggctg
     9601 gtacccaggg ccatgattac agagtcaacc gtaatctcga attcagaacc ttcgatgggc
     9661 accggcttcc tacgtccgga ctgatcaggc tcatcaagcc gcatccggac gcaacggatt
     9721 cccctgacat tgccttcctt gtcgccaagg atctcaaccg ggttagtgag gaagtcaaag
     9781 atcaccccct cttcttccgc atggtgaaac tcctcctccc tcgcagggac ctcgtccctt
     9841 gaccgacggt atacgagatg cacctctcct gaattctgtc cggcttcccg cgcctgcagg
     9901 gcctgtaaac ggaggctgca acgtacagag tccatggcca cgttgccagc gcccacgacc
     9961 gcaactttat tgcccacttt gaccggcgtg tcatattcgg ggaagcggta tcccttcatt
    10021 aaatttacgc gggtgagaaa ctcgttcgct gacatcaccc cgttgagatt aatgccaggt
    10081 atgcgcataa aggagggcag tccggctcca gttcccaaga agaccgcatc atactccttc
    10141 aagagttctt caagggtgaa ggtgcgacca atgacatggt ccaccttaat atcaatgccg
    10201 agacattcga cggcgtaatt aatttcgccg tgcacgacac tcttgggaag tcggaattca
    10261 gggataccat agatcaatac gcctccaggg ccgtgaaggg actcaaatat ggtcacttca
    10321 tgcccctctc tggccacatc cacggcgcat gtcagtcccg ccggaccaga gcccaccacc
    10381 gcaactcttt tccctgtctt tggcgctttc aagagaggac aggtccgcat ggcgagttcg
    10441 taatccccca caaaacgttc caatctgccg atggcgaccg gttcaccctt cttgcccaca
    10501 atgcaatttc cttcgcactg cacttcctgg ggacataccc ttccgcagat ggccggcaga
    10561 ttgttcttca ccttcatcca ttccaccgcc ttcacaatat cgccctctct gagggccttt
    10621 ataaactgcg gaatgaggac acccacgggg cagccctgca cacacttcgg gtccttgcac
    10681 tgcagacacc gctcggcttc ctcacgggca gtctccagac tgtagcccag ggcaacttca
    10741 tcgtagtttc ctctgcggac atgctgatcc tgattgctca tctcatgccg ctttattttc
    10801 atgcgttcct tctgagtcag ttcagccatt acttacctcc tcctggcagt tccccctcca
    10861 gtttgcactt gtgctcccat cgctccactg ccagccgttc ctcttttttg tagaactgca
    10921 gacgggacag aagactctcc cagttcacct tatgggcatc gaattccggt ccgtcaacgc
    10981 acacgaattg agctccctcg tcgagcagta cccggcatcc gccgcacata cccgtgccgt
    11041 cgatcatgat ggtattgagg cttaccactg ttggg
//
LOCUS       DATKOU010000125         4511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2857012, whole genome shotgun sequence.
ACCESSION   DATKOU010000125 DATKOU010000000
VERSION     DATKOU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857012"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VMU21_05815"
     CDS             <1..187
                     /locus_tag="VMU21_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77077.1"
                     /translation="PPPLTCYTTSKIQENLSSVKIFFAIAESLDFAQRQNWGMASPGG
                     VLTKKYFLAILEQREIS"
     gene            277..1527
                     /gene="rho"
                     /locus_tag="VMU21_05820"
     CDS             277..1527
                     /gene="rho"
                     /locus_tag="VMU21_05820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HUO77078.1"
                     /translation="MSISELKEKTIDDLTKVAKELNVEGAAGLRKQDLIFAILQAQIE
                     KTGNVFGAGVLEILPDGFGFLRSPDYSYLPSPDDIYVSPSQIRRFNLRTGDLVSGQIR
                     PPKEGERYFALLKVEAINHESPEENISRSLFDNLTPYYPTERIKLEYNGGDFSTRVME
                     LITPIGMGQRGMIVAAPRTGKTMLLQSIAKGIKKNHKEIHLIILLIDERPEEVTDWKR
                     QVSAAEIISSTFDEPPQRHCQVSEMVIERAKRLVESKRNVVILLDSITRLARAYNAII
                     PTSGKVLSGGLDANALQKPKRFFGTARNIEDGGSLTILATALVDTGSRMDDVIFEEFK
                     GTGNMELHLDRKLVDKRIFPSIDINGSGTRKEELLVDKDTLNKMWILRKVLNPLSTVE
                     SMEFLLGKLMGTKSNKEFLEMMNK"
     gene            complement(1680..2786)
                     /locus_tag="VMU21_05825"
     CDS             complement(1680..2786)
                     /locus_tag="VMU21_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO77079.1"
                     /translation="MNTDTAHLPSTTHSTGFNVLVVDDDFFVRGSISSLLREYGYTVF
                     SFENALDAIDALGHNNINAVLSDIKMPGISGIELLERIRGISPDIPVVLMTAYAELDM
                     AVDAIKKGAFDFIVKPFKADYLIHAMEKAVKYNNLLKIEKSYKQILEDTVRKRTAELT
                     KALEMVGNMSREIIQRLTSVAEFRDTNTGAHISRIGLYSKKIAETMSMPKDFVDTIAF
                     ASPMHDIGKVGVPDRILLKPARLTEEEFSVMKSHTVIGDKMLAGSSHPGIQMASSIAL
                     NHHERWDGTGYPGGLKAEETPVEGRIVMLSDQYDALRSKRPYKPALSHREAFRIITKG
                     GGRTMPGHFDPRVLNAFVQVAPAFEDIFNTHQDS"
     gene            complement(3128..4129)
                     /locus_tag="VMU21_05830"
     CDS             complement(3128..4129)
                     /locus_tag="VMU21_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HUO77080.1"
                     /translation="MTRVQRQFIISLLLIGVIIIFGVLGYVVIEGWTFFDALYMTITT
                     ITTVGYSEVHRLSDMGRSFTMFLIVFGVATILYTFNNAARIVIEGEMQEVFGRRKMEK
                     TIKGLRDHYVVCGYGRMGTVICKELKEKGARFVVVEKNPESRETFDGDMLLVKGDATK
                     DEVLREAGIEKAKGLISVLPTDAENLYVVLSAKVLNPNLTIVARAGEEGSEQKLLRAG
                     ADRVVSPYHIGGLRIAHTLLKPSVVDFIEFATRSGNIDLQMEEIYVREGAGIAGSTLD
                     QCGIGRELGIIIVAIKRTDGDMKFNPTFKTVIKPGDILIALGERTKLKTLEDMAEGR"
     gene            complement(4132..>4511)
                     /locus_tag="VMU21_05835"
     CDS             complement(4132..>4511)
                     /locus_tag="VMU21_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HUO77081.1"
                     /translation="DLMKLAVQEGRCLLTRDTHFLHIRNFRNFLMVHSDDPIEQVMEV
                     LCSFNLNEFKPGRCARCNGVLDPVTRREEVRNMVPEYVFLQCLSFLKCSGCGSVYWEG
                     THLKRFRTMLNPVLMSVKRKGLP"
BASE COUNT         1125 a         1203 c         1039 g         1144 t
ORIGIN      
        1 acctcctcct cttacttgct atactacaag taagatacag gagaatcttt catctgtcaa
       61 gatctttttt gctatcgccg aatctttaga tttcgcccag cgtcaaaact gggggatggc
      121 aagtcctgga ggggtattga caaaaaagta ttttttagct attttagaac aaagagaaat
      181 ctcctagagt agaacccccc tggataggta agcacctacc gggtaaacaa aaaccaccag
      241 atgaagaaat ccaaagaggt gcacacaatg gacaatatct caatttccga acttaaggaa
      301 aagaccatcg acgatctcac caaggtagca aaggagctga atgtggaagg cgcggcaggc
      361 ctccgtaaac aggacctgat ctttgccatc ctccaggccc agatcgaaaa aaccggaaac
      421 gtgttcggcg caggcgtgct cgagatcctc cctgacggat tcggattcct caggtctcct
      481 gattacagct atctccccag ccctgatgat atctatgtgt caccatccca gatacggaga
      541 ttcaatctga gaacaggaga ccttgtctca ggccagatac ggccgccaaa agaaggcgag
      601 cgctactttg cccttctgaa ggtggaagct atcaaccacg agtcccccga ggagaacatc
      661 agccggtccc tttttgataa tctgaccccc tactatccca ccgagcgtat caagctggaa
      721 tacaacgggg gcgacttctc cacaagggtt atggaactga tcacgcctat aggaatggga
      781 cagcgcggga tgatcgtggc agcaccaaga acaggaaaga ccatgctcct ccagtccatt
      841 gcaaagggga taaagaaaaa ccataaggag attcacctca tcatactgct catcgatgaa
      901 aggcctgagg aagtcacgga ctggaagcgc caggtgtccg ccgcggagat tatcagctcc
      961 acgtttgacg agccgccgca gcgccactgc caggtctcgg agatggtcat cgagagggcg
     1021 aagaggctgg tagagtccaa aagaaacgtg gtaatcctgc tggacagcat cacaaggctc
     1081 gcacgcgcct acaacgccat tatccccaca agcggcaagg tcctctccgg aggtctggac
     1141 gcgaatgcct tgcagaaacc aaagcgtttc tttggaaccg ccaggaatat tgaagacgga
     1201 gggagtctca ccatactcgc caccgcactg gttgacacag gaagcaggat ggatgacgtc
     1261 atattcgagg agttcaaagg caccggcaat atggaactcc accttgacag gaaactggtg
     1321 gacaagcgga tattccccag catcgacatc aacggctctg gcacgaggaa ggaagaactc
     1381 cttgtggaca aggacacatt aaacaagatg tggatactga ggaaggtgct taacccgctc
     1441 agcacagtgg agagcatgga attcctcctg ggtaaactca tgggcaccaa gagcaataaa
     1501 gaatttctcg agatgatgaa caaatagtct gtccggaacc cctccaacct ctttccctcc
     1561 tgtgcttttt gagtaatgcc tctttactct tcatcgggat aaggggaata atctgttgaa
     1621 cgttctttcg gaagtgtgtg tggcgagtaa tagtcttacg gagtatttcg cttctgtccc
     1681 taagaatcct ggtgagtgtt gaagatatct tcaaatgccg gggcaacctg aacaaaggca
     1741 ttcagcaccc tggggtcaaa atgtccgggc atggttctgc ctcccccttt cgtgataatc
     1801 ctgaacgctt cacggtggct cagggcaggt ttgtaaggtc ttttactcct caaggcatca
     1861 tactggtccg aaagcatcac gatccttccc tccaccggtg tctcttcagc cttgagtccc
     1921 cccgggtatc ctgttccgtc ccatcgttcg tggtgattta gggcaatgga tgatgccatc
     1981 tgtatcccgg gatgggaaga acccgccagc atcttgtcac caataacggt atgtgacttc
     2041 atcacgctaa actcttcttc tgtgaggcga gccggcttca ggaggattct gtcgggaact
     2101 cctaccttgc ctatgtcatg catggggctt gcgaaggcaa tggtatccac aaaatccttg
     2161 ggcatactca tggtctcggc aattttcttc gaatagaggc ctattcttga gatgtgggcg
     2221 ccggtgttcg tgtccctgaa ttccgcaacc gaggtgagcc gctggatgat ctctctgctc
     2281 atattaccca ccatttccaa tgcctttgtc aattctgctg tcctcttcct caccgtatct
     2341 tccagtatct gcttgtagct cttttctatc ttgaggagat tgttatactt caccgccttt
     2401 tccatggcat ggatgagata gtcagcctta aagggcttga caataaaatc aaaggcgcct
     2461 tttttgatcg catcaaccgc catgtccagc tctgcatatg cggtcatcaa tactaccggt
     2521 atgtccgggc tgatgccacg gattctttcc aggagttcaa tcccggaaat tccgggcatt
     2581 ttgatatcac tcaacactgc gttaatgttg ttgtgcccca gtgcgtctat agcatccagg
     2641 gcattttcaa agctgaagac cgtgtatcca tattccctga gcaatgaaga aatggaacca
     2701 cggacaaaaa aatcgtcatc taccacgaga acattaaacc ccgtactgtg ggtagtggag
     2761 ggaaggtgtg cggtatccgt attcattctt cttccttcac gtgtctttcc ctaatcatag
     2821 ttcctttaca ctgccctttc gcatccgttg tagcgcgaaa ggcatgctca tctgctccct
     2881 catatatata ctattccacc ctatttcgaa tatttctatg catttcttgc ttttttttat
     2941 gcattttttg cagggcgaaa aaagaagcgg ctctcatctg gtctgcgcag aaagaacaca
     3001 agagataata aaattcaaaa tattcaagta cttagggctt cgggagacag gggagttcat
     3061 tcagatgagg tgggtaagtt cctctcgttg cagcacctct gtggtaacag gcctcatgaa
     3121 gaaaaggtca tctgccctcg gccatatctt cgagtgtttt cagctttgtt cgctctccaa
     3181 gcgcgatgag gatatctcct ggctttatca cagttttaaa tgtgggatta aatttcatat
     3241 cgccgtcggt cctcttgatg gcaacgataa taatgcccag ttccctgcct atgccgcact
     3301 ggtcaagggt cgagcccgcg atgcccgccc cctcacgcac ataaatctcc tccatctgga
     3361 ggtcgatgtt tcctgacctt gtggcgaatt cgatgaagtc aactacggat ggtttgagta
     3421 atgtatgggc aattctcagc cccccgatat gatagggtga cacgaccctg tctgcccctg
     3481 ctctcaggag cttctgctca gagccttcct ccccggccct cgcaacaata gtgaggttgg
     3541 ggttgagcac ctttgcactc agcaccacat agaggttttc tgcatctgtg ggcagcaccg
     3601 atatcagccc cttcgccttt tcgatacccg cttccctcag cacctcatcc tttgtggcat
     3661 cccccttcac aaggagcata tctccatcga aagtttcacg gctttcagga ttcttctcca
     3721 ccactacaaa ccttgctcct ttttctttca actctttgca gatgacggta cccatcctcc
     3781 cgtatccgca caccacgtaa tggtccctta accctttaat cgtcttttcc atttttctcc
     3841 tcccgaatac ttcctgcatt tccccctcga taacgatcct cgcggcgtta ttgaaggtgt
     3901 agagtatcgt ggccacccca aagacaataa ggaacatggt gaacgatctt cccatatccg
     3961 aaagtctatg gacttcgctg tatcccactg tggttatcgt ggtgatggtc atgtaaaggg
     4021 catcaaaaaa agtccatccc tcgattacga catagcccaa cacaccaaag atgataatta
     4081 ccccgatcag caaaagtgag atgataaact gacgctggac ccgtgtcatc ttcaaggtaa
     4141 accctttcgc ttcactgaca tgaggactgg attgagcatt gtcctgaatc tctttaggtg
     4201 ggtcccttcc cagtatacgc tcccacatcc ggagcacttt aaaaaggaaa ggcactggag
     4261 gaatacatac tcgggaacca tatttctcac ctcttccctg cgtgtgaccg ggtcaagcac
     4321 cccgttacag cgcgcacatc tgcctggttt gaattcgttc aggttgaatg agcagagcac
     4381 ctccatgacc tgttctatgg gatcatccga gtgcaccatg aggaagttcc tgaaattccg
     4441 gatgtgcagg aagtgcgtgt ctctcgtgag aagacacctg ccctcctgca ccgccaattt
     4501 cataagatcg c
//
LOCUS       DATKOU010000126         5974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4399045, whole genome shotgun sequence.
ACCESSION   DATKOU010000126 DATKOU010000000
VERSION     DATKOU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399045"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..2293
                     /locus_tag="VMU21_05840"
     CDS             425..2293
                     /locus_tag="VMU21_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO77082.1"
                     /translation="MIQVSNLSKAYGTQVIFDDVGFTVNAGERIGLVGRNGHGKTTLL
                     RMITGEERPDAGVITVPDNYTVGYLSQHLVFSEDTVLKEACLSLRSSEDGRDESYKAE
                     TILMGLGFSSDDFPRHPLDLSGGYQVRLNLAKLLVSEPNLLLLDEPTNYLDIVSIRWL
                     TKFLKSWKGELILITHDREFMDSVTTHTMGIHRTKIRKIAGPTHKLYQQILMEEEVYE
                     QTRVNEEKKREEIQQFINRFRAQATRARSVQSKIKALQRKEKLEKMSRVKDLEFAFMP
                     APFTGKSLMQARDISFSFTQEGPPLIEGLNLTIGKRDRIAVIGKNGKGKTTLLNLLAG
                     ELQPLTGEVLHHSQLRLAYFGQTNIQRLSPDKTVEEEILDVQPEYHRSAARTICGIMM
                     FEGDRALKKVSVLSGGEKSRVLLGKLIVSPANLLLMDEPTNHLDMESVDSLLEALEVF
                     EGAVVIVTHSEMILHALSTRLIVFDDDRVKVFEGTYQDFLERDGWKDEGEGAAITGQH
                     TEKKRGTDRKELKRIKAELIDNRSRVLVPLQTRIVEVEEAITQLEQGMAQDTQALVDA
                     SVKGDGESIKRLSKSIHEHRGKIDALFDELETLTNELDARSRDFEERLDSLKTAGG"
     gene            complement(2304..3632)
                     /locus_tag="VMU21_05845"
     CDS             complement(2304..3632)
                     /locus_tag="VMU21_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUO77083.1"
                     /translation="MRFEELNIPQAVLEGIRRAGFTECTPVQAEVLPQALKGSDIAAQ
                     AQTGTGKTAAFLIAIFSRMLTTRAPGPGVSPRALIIAPTRELVVQIEAEAKILEGSAG
                     FRIVPVFGGIDYLKQRQEIVKGVDVMIGTPGRLIDYLKQNVYSLRRTQFLVIDEADRM
                     FDMGFISDLRFLLRRMSPYNRRQSMLFSATLSHRVMELCYEHMNLPERFSITPEQVTV
                     EKIEQEIFHVGKDEKFGLLLGILRHEQGGRYLIFSNMKSTAERLELLLNANGFAASAI
                     TGDLPQKKRMKVLSHFKDGTLPILVATDVASRGLHIEGVTHVINYDLPQDREDYVHRI
                     GRTARAGAEGRAISLACEECVHSLPDIEEYIREKIPVVAVTDEMIVTDYKRVSARHRG
                     KPFPPQRKEREKREAGKQKPMRTGSNASRSERPRTHHRRDEKKPRPSPRS"
     gene            3973..4287
                     /locus_tag="VMU21_05850"
     CDS             3973..4287
                     /locus_tag="VMU21_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77084.1"
                     /translation="MATVRNREFRNYCDAMYRELSGMRSKLLGFVNDIEHMKGPESDV
                     LKPHIPHFQDIARTIDWKLEILTRVCPFDWTGYAGVEKTASVQVQELSEKEPIAAGYL
                     GG"
     gene            complement(4549..4953)
                     /locus_tag="VMU21_05855"
     CDS             complement(4549..4953)
                     /locus_tag="VMU21_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77085.1"
                     /translation="MKMVLINYSGLRDPEGAKTSPPDKGSGKFVQIRKSEIEYLVFSP
                     EELAPYHAHIVERFCIERGIRGAHEVEGKRFSIHDPGWTVVGGGKYEIDQEHRYIRLY
                     DDSVAYGKFDSKGLKEKIHTIIELRGFEVRIE"
     gene            complement(5116..5706)
                     /locus_tag="VMU21_05860"
     CDS             complement(5116..5706)
                     /locus_tag="VMU21_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77086.1"
                     /translation="MGEVKSAFEKAMEKIKEIQGLGPEEKDEMKDRQKLRSVLAAFYK
                     GDIKRDEIWQKLKGMKPSLLKEAQENMVNSLRMGNIPEEFQIRKDGILAIEALKDRQN
                     MTAIENSLSAVDRVQREYIDIRQRAVEELRAAIEENPKLRVRPVRTPDGRTVLQATPS
                     VDEAVQARVGEFLAEHEKRYEAMFFKAIERLKKELK"
BASE COUNT         1486 a         1544 c         1451 g         1493 t
ORIGIN      
        1 gagaaaatgc tgatgtcaac ttataaattt ggtttgtcag gacttgagtt gtttagcttt
       61 aatgtcaaaa ggcgccttcc ctactgatta ccgtttgtaa ggttcagtta tgagacctgc
      121 ataagaagtg gttggttcag gaaggaacaa aaactcgagt ttcagttgtt ttcagtggaa
      181 gtcctcatct caaacaaact gctctgagtg cgtgttagaa ggtgtaggtc tcaaccgcct
      241 tgttattgaa tagcaaaaga gggagtaacg ggttattctt gttgcaaact tctggtatga
      301 ttccgggcag cagatttcag gcagaatggt aagcctgcga tctctccgga tcgccctcca
      361 atgcttgtcc atgtagttca ttactccttt gatgagtgcg attcatttca gttagaatat
      421 tgagatgatt caggtcagca atctttcaaa ggcatatggc actcaggtca tttttgacga
      481 cgtagggttt acggtcaatg caggagagcg tatcggcctg gtgggcagga acggccacgg
      541 caagaccacc ctcctcagga tgattacggg agaggaacga ccggacgcag gcgtgatcac
      601 tgtccccgac aattacacgg tgggctatct ttcccagcac cttgtcttca gcgaagatac
      661 agtcctcaag gaagcctgcc tcagcctcag atcttctgaa gacggcaggg acgaatcgta
      721 caaggcagag accatcctca tgggcctcgg gttttcctca gatgattttc cgcgccatcc
      781 ccttgatctt tccggaggat accaggtccg tctgaacctt gcaaagctcc tcgtctctga
      841 accgaacctt ctcctcctgg atgagcccac aaactacctc gacattgtct ccatacgatg
      901 gctcacgaaa ttcctgaagt cctggaaagg agaactcatc cttattaccc atgacaggga
      961 gttcatggac agtgtgacga cccacaccat gggcatccac cgcacaaaga taaggaagat
     1021 tgcgggcccc acccacaaac tttaccagca gatactcatg gaggaagagg tttatgagca
     1081 gacaagagtg aacgaagaga aaaaacgcga ggaaatacag cagttcatca acaggttcag
     1141 ggcccaggcg acccgggcac ggtcggtcca gtcgaaaatc aaggcactcc agaggaaaga
     1201 gaagctcgaa aaaatgtcaa gggtaaaaga cctggaattt gcatttatgc ctgctccctt
     1261 tacagggaaa tcccttatgc aggccaggga catctccttt tcctttaccc aggaaggtcc
     1321 gccccttatc gaaggcctga atctgaccat cggaaaaagg gaccgcatcg cggtaatcgg
     1381 gaagaacggg aagggcaaga ccacactgct caacctcctc gcaggggagt tgcagcccct
     1441 cacgggtgag gtgctccacc attcacaatt gagactcgcg tacttcggcc agaccaacat
     1501 ccagcgtctc tcccccgaca agacagtgga agaggagata ctggacgtgc agcctgaata
     1561 ccacaggagc gctgcacgca ccatttgcgg cattatgatg tttgagggcg accgtgccct
     1621 caaaaaggta agtgtgcttt caggaggtga gaaaagccgg gtgcttttgg gaaagcttat
     1681 cgtcagccct gcaaacctgc ttcttatgga tgagcccacc aaccatcttg acatggaatc
     1741 cgtagattca ctgcttgagg ctttggaggt atttgaaggc gcggtggtca tcgtcaccca
     1801 cagcgagatg atcctccatg cattgtccac aaggcttatc gtattcgatg acgaccgcgt
     1861 gaaggtattt gaaggcacct atcaggactt ccttgagagg gacggctgga aagatgaagg
     1921 tgaaggggca gcgatcaccg ggcaacatac agagaagaag agaggcacag acagaaaaga
     1981 gttgaagagg atcaaggctg aattgatcga caaccggtca agagttctgg tcccccttca
     2041 gacaaggata gtcgaagtgg aagaggcaat cacgcagctt gaacagggga tggcgcagga
     2101 tacgcaggcc cttgtggatg cttcggtaaa gggagacgga gaatccataa agagactttc
     2161 caagtccatt catgagcata ggggaaaaat agatgccctc tttgacgaac tggaaaccct
     2221 caccaatgaa cttgatgcac gatcaagaga ctttgaggag aggcttgatt ccctgaagac
     2281 agcgggaggt tagaaaaagc gctctacgac cggggcgaag gacgaggttt cttttcatcc
     2341 ctcctgtggt gtgtgcgcgg tctctccgac cgggacgcat tgctgccagt tcgcataggt
     2401 ttctgcttcc cggcctctct tttttccctt tcctttctct ggggcgggaa tggcttcccc
     2461 cgatgacgtg ccgagaccct cttataatcg gtgacgatca tctcgtcagt caccgccacc
     2521 acggggatct tttccctgat atactcctcg atatcaggga gcgaatgcac gcactcttca
     2581 cacgcaaggc ttattgccct tccctctgcg ccggcacggg cggtcctgcc gatgcggtgc
     2641 acgtaatcct cacgatcctg ggggaggtcg tagttgatca catgagtcac cccttcgatg
     2701 tggagtccgc ggcttgccac atctgtggcc acgaggatag gaagcgtgcc gtccttgaaa
     2761 tgcgagagta ccttcatcct ctttttttgc ggaaggtcac cggtgatggc tgaggcggca
     2821 aacccgtttg cgttcaggag aagctccagc cgttccgcag tggatttcat gttgctgaag
     2881 atgagatagc gtcccccctg ctcatgcctg aggataccca gaagcagtcc gaacttctca
     2941 tcctttccca cgtgaaagat ctcctgttca atcttttcca ccgtgacctg ctcaggggta
     3001 atgctgaacc tctcgggcag gttcatgtgc tcatagcaga gttccatcac gcggtgggag
     3061 agggttgcgg aaaagagcat ggactggcgc cggttatagg gtgacatcct cctgaggaga
     3121 aaacggaggt cgctgatgaa tcccatatca aacatccggt ccgcctcgtc tataacaagg
     3181 aactgtgttc ttctcagact gtacacattt tgcttgagat agtcgataag ccttccgggt
     3241 gtgccgatca tcacgtccac acccttcacg atctcctggc gctgcttcag gtagtcgata
     3301 ccgccaaaaa cgggaacgat cctgaaccct gcagaccctt ccagtatctt tgcctcggcc
     3361 tcgatctgca ccacaagttc ccgcgtgggt gcaatgatga gggccctagg agacacaccg
     3421 ggtcccgggg ctcgcgtggt gagcatccgt gagaaaatgg caatgagaaa ggctgcggtc
     3481 ttgccggttc ctgtctgggc ctgggcagca atgtcacttc ccttgagggc ttgaggaagc
     3541 acctcggcct gtacaggcgt gcactccgta aaccccgccc gccttatccc ctccaatacc
     3601 gcctgaggga tattcagttc ttcaaacctc atacattacc taacattata atacaaaaag
     3661 aaaagattgt cggcttcctc catgaggaca acatgtgaac atgttgtatc tgtgcggaat
     3721 tactcccttc cctgattaag atctgccggt gacaggtgat attcccaaaa agaacaatac
     3781 attgccgagc gcatacacaa gaacacaggg tggataaatc tttcttttgt ttccgagggc
     3841 ttagtttatt agttgctaat atgcttgaca agttatatgt tctgtgctat agttaaatca
     3901 acaagggaga gtaaccatag taagtaaggt taccctttta gtaacggtgt tcattcctga
     3961 aaggaggaac ccatggcaac cgtaagaaac agagaattca gaaactattg cgatgcaatg
     4021 tacagggagc tttccggcat gaggtcaaag ctgctcggat tcgtgaatga catagaacat
     4081 atgaaaggtc ctgagagtga cgtgctgaaa ccgcacattc cacatttcca ggatattgca
     4141 aggaccattg actggaaact ggaaatactc acaagggtct gtccttttga ctggaccgga
     4201 tatgccggtg tggaaaaaac cgcttcggta caggtgcagg agttgtccga gaaggaaccc
     4261 attgcagccg gttatctcgg gggataacaa gaccccctac actttgcagg gctcttatga
     4321 ctggcgggga tgaaacattt ccgccagttt tttattttcc tttagcggaa aaaacccaaa
     4381 gccctcggtg caacgttcgg ggggaaagag acaatacgca ggaagaccat ttcagcgggt
     4441 acgagcggat gaggggtatc caaggacgca ttacttcttg ttcgttgctg ccgcagtcgc
     4501 tttgatcacg atacaacgtc ggtgtcttta cgaaagcctc ctgagatatt attcgatcct
     4561 tacctcaaac ccgcggagtt cgataatcgt gtgaattttt tccttcaatc cctttgaatc
     4621 gaattttccg tatgcaactg aatcatcata gagacggata tatctgtgct cctgatctat
     4681 ctcgtacttc ccccctccca ctactgtcca gcctgggtca tgaatggaaa acctctttcc
     4741 ctcaacttcg tgagcaccgc gaatccctct ttcgatacag aagcgttcca cgatgtgggc
     4801 atggtagggg gccagttcct cgggggaaaa caccagatac tcgatttcac tctttcttat
     4861 ctgaacaaat ttcccgctcc ccttgtccgg tggagaggtc tttgcccctt ccggatcacg
     4921 aaggccggaa taattaataa ggaccatttt catacttgtt cctcaatgaa caagtatagc
     4981 agaaatgagg cagcacttat tatggttatg tcagggcaaa gggaagcaca cagcaatttc
     5041 aattctcaac aactgtgagc ttcccctcca gtatcaggtc gggcttcaaa gcaataaaaa
     5101 acagcggtgc tttttctatt tcagctcctt tttcagccgc tcaatggcct taaagaacat
     5161 tgcttcatac ctcttttcat gttctgccag aaactcccct acccttgcct gtaccgcctc
     5221 atccacggac ggggtggcct gcagcacggt cctgccatca ggggttctca ccggcctcac
     5281 cctcagcttg ggattctctt caatggctgc cctgagttcc tccacagccc tttgccttat
     5341 atcaatatat tccctctgca ccctgtcaac agcgcttaag gagttttcga ttgctgtcat
     5401 gttctgtcta tctttcaacg cctcaatggc aaggataccg tccttcctta tctgaaactc
     5461 ctcaggtata ttgcccattc tcagggaatt aaccatgttc tcctgcgcct ctttcaggag
     5521 agagggtttc atgcccttta acttttgcca tatttcgtcc cgtttgatat cacccttata
     5581 gaaggccgcc agaactgatc tcagcttctg acgatccttc atctcatcct tttcttccgg
     5641 gccaagcccc tggatctctt ttatcttttc catagccttt tcaaaagcgc tttttacctc
     5701 tcccatgttc agctgctcct ttcttctgcg gataggaatc ttccttaatt ctaacaaact
     5761 tcactcagtg tagccactat tttaaccaaa acatgacaca ccttttccac acatccaagg
     5821 catggagcgc aaaagttctt tgcataaaac cgcttctgtc cacgtcctgc tgcctttaca
     5881 cataaaaggg gtgatcatgc accgatgatc cttgcttgca aaaatgtgcg ggttattgct
     5941 tgacaactgg ctatatattg ggttagatta taca
//
LOCUS       DATKOU010000127        13882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5168994, whole genome shotgun sequence.
ACCESSION   DATKOU010000127 DATKOU010000000
VERSION     DATKOU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5168994"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VMU21_05865"
     CDS             <1..732
                     /locus_tag="VMU21_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77087.1"
                     /translation="TGKTSVAALTVRYLTEKRKGPVLAVDADSNACLNEALGISVHTT
                     IGHLREESLEAVRSGGDRPGGMSMEQLFDYQVHQSVIESKGVDLIVMGRPEGPGCYCA
                     ANNIIRKYTDKLSETYPYVVIDNEAGMEHLSRRTTHKVDLLFMVSDPTVKGVLTAKRI
                     NALVDELDLEIEKRVLLINRVAGPDGEKLKTLAESYGLTVGGLIPQDNTIFEYDLQGL
                     PIAALPSDSVALGSIYQVLDSLGIP"
     gene            complement(1693..2577)
                     /locus_tag="VMU21_05870"
     CDS             complement(1693..2577)
                     /locus_tag="VMU21_05870"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HUO77088.1"
                     /translation="MDIHHLRVFVTVFRNRSFSRASEELHLTQPTVSDHVKSLEEELY
                     CRLFDRLGRSIAPTKEADILYSHAAEIIEKTDALKELIGQFKKEITGELIVGASTIPG
                     TYVIPRIIAGFKKRYPSVSFQIIESDSKGVIEKVLRHELLLGMVGAKMGHGQLTFTPV
                     MEEELIAVSSPSLIRAHEMSLEELTSFPMILREEGSGTRKEMERILEGRGISMDALKV
                     VGCFGSTDAVKQAVKAGLGVSILSELAVADELKYKSLKKIKVKRVQMKRKFYAVTHRK
                     RTLPFAYSIFMEYLKAHL"
     gene            2758..3291
                     /locus_tag="VMU21_05875"
     CDS             2758..3291
                     /locus_tag="VMU21_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HUO77089.1"
                     /translation="MCCSNCSLYPMKSLLSKGVDVKGLWFNPNIYPLPEYELRLASLQ
                     DLQRLWHIDVEYVDKYGSDDFVRELGNSHDNRCIRCYTVRLEKTAKTAKKMNLKGFTT
                     SLLVSPYQKFDSIVSVGKEMEKRYSIPFYVEDFRVGYRENIPLSKELGLYRQKYCGCV
                     FSQQESTRQRTKIRTLQ"
     gene            3314..3541
                     /locus_tag="VMU21_05880"
     CDS             3314..3541
                     /locus_tag="VMU21_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HUO77090.1"
                     /translation="MNEGVQHIGKFLIITGIIIAAVGALLLLSGKIPWLGKLPGDIVI
                     ERKNFTFYFPLATSILLSIIITFIFWLLGRR"
     gene            3549..4541
                     /locus_tag="VMU21_05885"
     CDS             3549..4541
                     /locus_tag="VMU21_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HUO77091.1"
                     /translation="MGKYLLSVMATILVLSPVAYTAMNPAKIKVLILEDSRSTIPDKD
                     EKLVKLGNMQGDLLMTGIHYSGNIEVWKGDKGLYIVNELPLEDYVESVVVAEVGTNWD
                     LEALKAQAVITRTYAIYKKAMSQDSRFHLTSSVLHQVYKGNNPHILISYAVRETAGEV
                     LMYNGKPIEALYHSTSGGRTENPEEVFGKSYPYLKSVESNCEISPYWVWEKKIPKEEI
                     EKALGIKNLKDITITTFTSTGRVKELTIGLESGQMSMKATDFRKLLGWSRLPSTSFRI
                     HSDETTIAFEGKGYGHGVGLCQWSALQMARDGKSYREILSFFYPGTEIQLYEGD"
     gene            4528..5586
                     /gene="queA"
                     /locus_tag="VMU21_05890"
     CDS             4528..5586
                     /gene="queA"
                     /locus_tag="VMU21_05890"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HUO77092.1"
                     /translation="MKVTDLDFHLPESLIALRPVLPRDSCRLLVLHRDGSVEHTRFSH
                     LPAFLHEGDLLLLNNTKVFPARLTGTKKTGGRIELLLVRETEPGLWEVLTREKYTGSV
                     FISEELSGELLEGRSLRISVQPTGIVCRREETSDFMKAVWRTGSMPLPPYIKRKPEEM
                     DKQWYQTVYAEHVGSIAAPTAGLHFTDSLIAGLEEKGVLIRFLTLHVGTGTFRPIRVK
                     DVEEHPMDAEYFEIDRSLMDTIDKVKGSGRRVIAVGTTTTRAIEGFASNRWKPAGENG
                     NSSGSGYIRGVSDIFIYPGYTFKVVDSLITNFHLPRSTPLLLTSALCGARNLMNAYHS
                     ALSIGYRFFSYGDAMLVL"
     gene            5583..6338
                     /locus_tag="VMU21_05895"
     CDS             5583..6338
                     /locus_tag="VMU21_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUO77093.1"
                     /translation="MKRDAATIAELLPGKIVLIVITATAVTTGFTLGYFVGRSVSFSP
                     ESSMLKQSPSSDLAAVPSATNPSSELKNEASSPSLPKMHPPSKESTQASVPPSLGGAI
                     PLTADVRDEEKISPKSQKAAMTEKKAIPAAGEQSTFPSGVSNPDIETRNALNPQSTPK
                     SSPAPHAGSSHSEVVYTVQAAAFKNQKDADTLRQTLEGKGYKVFIKKESGPNGVVLFK
                     VRVGEFEQKKDASVFALKLMKTGGLNAFAVLKN"
     gene            6564..7331
                     /locus_tag="VMU21_05900"
     CDS             6564..7331
                     /locus_tag="VMU21_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUO77094.1"
                     /translation="MNPDDIRFAVRAISSGGEVSLRDLIMNHIPVSSKKRFIIPKTEV
                     QRDYVEAIKSHDIVIGIGPAGTGKTYLSMAMAINALLKKEVSRIVLARPAVEAGERLG
                     FLPGDMYEKVNPFLRPLYDALFDMMEPEKVGKLIERGVIEIAPLAFMRGRTLNDSFII
                     LDEAQNTTSEQMKMYLTRLGFSSKTVITGDTTQIDLPAGKSSGLIEVEKILEGIDGIK
                     FIYFSEKDVVRHRLVQEIVKAYERHEKRSSDHSKTQE"
     gene            7282..8892
                     /locus_tag="VMU21_05905"
     CDS             7282..8892
                     /locus_tag="VMU21_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HUO77095.1"
                     /translation="MKGMKNGAQIIQRPRNEQITTARKYTLLIALGLFTSLAIQTKIG
                     IESFIGGFLVASLLLFILYRDILRYKPQYMKKYPMLFLLGLMIITTIVLGRLSHYVLL
                     NLSKGFGSLAIEGAVFGMPIAAGAMLVTLMFDFHTAIFFSFIVSLLSGLWLLDAVYPV
                     YVFVGSLTAAFSVIRCKKRSAFLKGGLSVGGASIVTSILLLLSQGELFTEKAISTLIF
                     AASTGLSVPAIVSLLLPVLEYTFHVTTDISLLELLDLNQPLMKSLMINAPGTYHHSVI
                     VGNLVESAAEAVGVNPLLARVSAYYHDIGKIKMPEYFVENYTSAVGKHDRLTPHMSTM
                     IIGSHVKEGIELGYQHKLPQSIIDIIQQHHGTSLMTYFYQKAKDHDVGDHPLVEDYKY
                     QGPKPQTRIAALVMMADAVEASSRVLNDPTPARITALVDRIINHIFLEGQLDECELTL
                     KDISEIKKHFSYILTGILHKRIDYPGFDFNQEPKEIFKDSERGLGGDMPFTPHTHEDP
                     HREPPKSHKDKPQEAKRRLAGTSHDIPS"
     gene            8903..9244
                     /gene="ybeY"
                     /locus_tag="VMU21_05910"
     CDS             8903..9244
                     /gene="ybeY"
                     /locus_tag="VMU21_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HUO77096.1"
                     /translation="MGVLLVDDRQMKQLNCLHRSLNKTTDVLSFPIYERRVEIPVDRE
                     SLLGDIVINLDAAKRQSSLCGVTLFGEVRRLLIHGFLHLLGYDHERSLYQKKKMRRHE
                     NELRNALETLD"
     gene            9228..9920
                     /locus_tag="VMU21_05915"
     CDS             9228..9920
                     /locus_tag="VMU21_05915"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HUO77097.1"
                     /translation="MKRWIKSANFAIEGILHGTKTQRHLRYHLYSAAGVLILCYILGI
                     SRNDFLVIAVMVIVVLVAELFNTAIETMVDILSPKHSEKARIAKDVAAGAVLTTAFGA
                     AVIGYIILSPYIRKGFQTGFSIAKHSGEDISVMAVILVLILVVITKAYLGKGTPLRGG
                     IPSGHSALAFSLWVGVSYTTGNVLASAFCFTLAAVIAAGRVASKIHTVWEVVLGGAIG
                     ASLTFLLFQIFS"
     gene            complement(9950..10696)
                     /locus_tag="VMU21_05920"
     CDS             complement(9950..10696)
                     /locus_tag="VMU21_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUO77098.1"
                     /translation="MRHRTGKVTMAGAFACLVVVTILRMSYAQELKEEKGTISVMGEA
                     REYYPPDTAFITLGVETAGKTVSETVAENSKKSEGMVSALKRLISPAAGDSIKTSSYT
                     VQPVYEYDEVKKKSILTGYKVLNQVTVRTKKIEMAGKIIDNAMQIGANQAQGISFTLS
                     EEKEYCDGVIRKAAEGAKREAAFVARTLGTTISGIKSISPSCGRQPHPIYRQAVMETK
                     MAVAAPEISIEAGDIAVSATVNVVFYLDKQ"
     gene            10827..11663
                     /locus_tag="VMU21_05925"
     CDS             10827..11663
                     /locus_tag="VMU21_05925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUO77099.1"
                     /translation="MGLTFLIPALCVLIGYSCADAFQAGPLPSRISPGDAFLIKVSEA
                     RASEIPAASLNGKEILFSRCGETCFIGIGAAALETKPGRHVIYLRMGEKSTQLSLLVK
                     RTRFPTLFLTLPKEKVFLSPEDLERAEREEERLRLLWMTISDRLWEGSFLLPLEHSIS
                     SVFGTKRMMNRQVASIHKGVDIRGKEGDEVKASNRGKVVFAEELFFGGNTIILDHGQG
                     IFTMYMHLSSFRVKPEDVVSKGDIIGSVGSSGRSSGPHLHFGVKIQGVSVNPVSFVKL
                     TL"
     gene            complement(12137..12364)
                     /locus_tag="VMU21_05930"
     CDS             complement(12137..12364)
                     /locus_tag="VMU21_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77100.1"
                     /translation="MGERNNKLDELSNQLGENILAVKGTLELMDASVTEDDLHELLLK
                     AIERMDMIQRLSHEMLVALRNCFDKIDEIKK"
     gene            complement(12411..>13882)
                     /locus_tag="VMU21_05935"
     CDS             complement(12411..>13882)
                     /locus_tag="VMU21_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUO77101.1"
                     /translation="GVMSVQMEPSGEVINDLNPVCRKQPTHSYRSYLLSVSILLVFSI
                     SGCAHYPVNEPLKEYAPEAGYRAKNVRDPERSDDILFFVTFSGGGTRAAAFSYGVLEE
                     LAVREISINGRKRRLLDEVDTISSVSGGSFTAGYYGLFGERIFEDFEQRFLKKNVQGE
                     LVSRTFFNPVNWVRLFSPSFDRSDLAAEYYDKYIFEGGTFGDIAARKGPMVIINATDM
                     TYGIRVGFTQDMFDAICSDVSRFSVARAVAASSAVPIVLTPITVRNYAGSCNYRMPGP
                     IERVFKESQISERQFYLANNIAPYLDSKKKPFLHLLDGGVADNLGLRAILDRIIYRGD
                     FWKTIKGTHHENLHKVIFIVVNAETQPDSFWDRVESPPAFAAMLESYSSIAIERYNVE
                     TLALLKESVKGWADQVRTGRCPKGKISTRPGSCGDIQFYVIEVKFDALKDGEERLYFM
                     RLPTSFKLSPEEVDKLRDAAHRILAESREFQRLLKDLRE"
BASE COUNT         3641 a         3485 c         3238 g         3518 t
ORIGIN      
        1 acggggaaaa cgagcgtcgc agcccttacc gtacggtatc ttacggaaaa gcgcaaaggg
       61 cctgtgcttg ccgtggatgc ggacagcaac gcatgcctta acgaggccct cggcatctct
      121 gtccatacta ccatcgggca cctcagagaa gagtcccttg aagccgtgag gagcggagga
      181 gaccggcccg gtgggatgag tatggaacag ctctttgact accaggtcca ccagtctgtc
      241 atcgaatcca agggcgttga ccttatcgtc atgggaagac ctgagggacc cggctgctac
      301 tgtgctgcca ataacattat cagaaagtat actgataagc tttccgagac atatccctat
      361 gtggtcattg ataatgaggc gggcatggaa catctctcaa gacggaccac ccataaagtg
      421 gaccttcttt tcatggtgag tgatcccaca gtcaaggggg ttctgaccgc aaaaagaatc
      481 aatgcccttg ttgatgaact cgatctcgaa attgaaaaaa gggtgctcct tataaaccgc
      541 gttgccggac ctgacgggga aaaactgaag acattggctg agagttacgg actcactgtg
      601 ggagggctca ttccccagga caataccata tttgaatacg acctccaggg tctgcccatt
      661 gcagcacttc cctcagattc agtggccctc ggttccatat atcaggtcct cgactccctc
      721 ggaataccct aatcattcaa ttctaaccaa tccatgcact tttcttgcag tctctcatcc
      781 attcccggat gaatgagaaa attccgtggc aaccacaatt aacaggtcta tctccggttc
      841 tgtcaccatt aacggcttcg gacactcaaa tccccgagca tcaattcgca ttttgctcct
      901 gtgaagcatg aatccatacc taagtttatc caatggtctc cctgaggaca ttgagtacca
      961 gggtagcatc tggggggaaa aggacaagct agcacataag aaaagagaaa aatgagaacc
     1021 accttccatt tgatggtttt attactcagt caacgattcg ttcttaggcc aattcgagat
     1081 gacttgcaaa acctcacgca catacgcaca ttcacatttc gcgggacatg ggcgtaactc
     1141 attttcgtgc acacagaatc cctggcactc ttcggtgcca ttgagccttt cggtaaacat
     1201 cggcccacta tagaggtcct gaaactcttt tgtctgaacg gaaagcgtag tcccgcaact
     1261 gtggttgaat aagataaggc ctgcagtaag ctcttcaaag tggacttgat aaccaaccag
     1321 gtagagcgag ggatcactga gaaaagaagt ccttccgggc caaacaaacc cgcactgaga
     1381 gcatatcttg aatgatgcgg attgcagtct gctgactgaa atcattttat ctgcgttcat
     1441 tttctcccgg tattatatga tgattgtgtt atttccttca actgacacag ttacttataa
     1501 ctctcaatag gacagaaaag ccctccctcc ttaataattc acagccttcg gatataagcc
     1561 ttgcaggtaa aacctgcttg gtttttttaa agggaaaacc cacatggaag ggacaataac
     1621 ttcagggtga aaatgaagaa gcccgcagcg agtaacggga gacgcgcgcg ctatctgttt
     1681 caaaaagaat gttcacaaat gtgccttcag atattccatg aaaatgctgt acgcaaaggg
     1741 aagtgtcctc tttctgtgag tcactgcata gaacttccgt ttcatctgga cccgtttgac
     1801 ctttattttc ttcagtgatt tgtattttaa ctcgtcagca accgcaagtt ccgagagaat
     1861 ggataccccc aaccccgctt ttactgcttg cttgactgca tctgttgagc cgaagcaacc
     1921 caccactttc agcgcatcca tcgagatgcc ccttccttcc aaaatccttt ccatctcttt
     1981 tcgcgtaccg gagccctctt ccctcaaaat catggggaag ctcgtcagtt cctccaatga
     2041 catctcatgc gcccttatca atgagggtga agaaacggca atcaattcct cttccatcac
     2101 cggcgtgaag gtgagctgtc cgtgacccat ttttgctccg accataccca gaagcagttc
     2161 atgccgcaac actttttcga taactccctt agagtcggac tctataatct gaaaggagac
     2221 ggatggatac ctcttcttaa aaccagcgat gatacgcggg attacatagg ttcccggaat
     2281 tgtgctcgca cccacaataa gttcgccggt aatttccttt ttgaattgtc ctatgagttc
     2341 tttgagggcg tcagtctttt cgatgatctc cgcagcgtga ctgtaaagga tatctgcctc
     2401 ttttgtggga gcaatggacc ttcccaatct gtcaaaaagc ctgcagtaaa gctcctcctc
     2461 caacgacttc acgtggtcgc tcacagtggg ctgcgtgaga tggagttctt cggaagccct
     2521 ggaaaagctc ctgttcctga atactgtcac gaatactctg aggtgatgta tatccatacg
     2581 cagtgcggta acattatacc acacaccaga gagagtcaag cccctttgca gcaagcgaca
     2641 aagagcgtac ttgttctcca tttctttatg cgcagcagag agtgcgcaac gataacatga
     2701 aaaagttggg cggatttctg ttaatatata tatatatata tgaagctcct gatgcatatg
     2761 tgctgctcaa attgcagtct ttacccgatg aagagcctcc tctctaaggg tgtggacgta
     2821 aaaggattgt ggttcaaccc caatatttat ccccttcccg agtatgaact ccggcttgcc
     2881 tctcttcagg acctccagag gttatggcac atcgatgtgg aatatgtgga taagtacggc
     2941 tctgacgatt ttgtgaggga attggggaac tcccatgata atcgctgcat ccggtgttac
     3001 acggtacgcc ttgagaaaac agccaagact gcaaaaaaaa tgaacctcaa gggattcacc
     3061 acatcactcc ttgtgagtcc ttaccagaag tttgattcca tagtaagcgt agggaaggag
     3121 atggagaaga ggtattccat tcccttttat gtggaggatt tcagggtcgg gtacagggaa
     3181 aacatccctt tatcaaagga gctcggactt tacaggcaga aatattgcgg gtgcgtgttc
     3241 tcacagcagg agagtacccg gcaaagaaca aagatcagga ccttgcagtg aataacccct
     3301 gtgtggcaat ttcatgaatg aaggcgtcca gcacatagga aaattcctca ttatcaccgg
     3361 gattattatc gcggctgtgg gggctctact ccttttgtca gggaagatcc cctggcttgg
     3421 caaactcccc ggagacatcg taatcgagag gaagaacttc accttctatt tccccctcgc
     3481 aacgagcata ctcttgagta tcatcattac attcattttc tggcttttgg ggaggagata
     3541 agcagctcat gggaaaatac ctcttgtctg tgatggcaac catccttgtc ttatctcccg
     3601 tggcatatac cgccatgaat cctgcaaaga taaaggtgct tattctcgaa gacagtcgct
     3661 ccactattcc ggacaaggac gaaaagctcg tgaagttggg aaacatgcag ggggatctcc
     3721 ttatgaccgg aattcattac agcggcaaca tcgaagtctg gaaaggcgac aagggactct
     3781 atattgtgaa tgaactcccc cttgaggact atgtggaaag tgtggtcgtg gccgaggtgg
     3841 gtacaaactg ggatcttgag gcactcaagg cacaggcggt gattacccgt acctatgcga
     3901 tatataagaa ggccatgagc caggattcga ggtttcatct cacctcttct gtgttgcacc
     3961 aggtatataa gggaaacaat cctcatatcc tcatctctta tgcagtgaga gagacggcag
     4021 gtgaggtact catgtataac ggcaaaccta tcgaggccct ctaccactcg acatccggag
     4081 gcaggaccga aaaccccgaa gaggtgttcg ggaagagtta tccctatctc aagtcagtgg
     4141 agtcgaattg cgaaatttct ccttactggg tatgggaaaa gaagattcca aaggaggaga
     4201 ttgaaaaggc attaggaatt aagaacctca aggacatcac tataactacc tttacatcta
     4261 cgggaagagt aaaggagctc accataggtc tggagtccgg gcagatgtca atgaaggcaa
     4321 cggatttcag aaagcttctt ggttggtcga gactgcccag caccagtttc aggatacatt
     4381 ccgatgaaac caccatagcc ttcgagggaa agggctatgg acatggggtt ggcctctgcc
     4441 aatggagtgc cctccagatg gcaagggacg ggaaaagtta tagggaaatt ctctcctttt
     4501 tttacccggg gactgagata caactttatg aaggtgactg acctcgattt ccatctcccg
     4561 gaatccctca tagccctaag acctgttctc ccaagggata gctgcaggct cctggtcctc
     4621 cacagggatg gctcagtgga acacacaaga ttcagccatc tgcccgcatt tctccatgag
     4681 ggggacctct tgcttttgaa taatacaaag gtatttcccg ctcgcctcac aggcaccaaa
     4741 aagacagggg gaaggattga actcctcctg gtgcgggaaa cggagcccgg tctctgggaa
     4801 gtcctcacca gagagaagta taccggcagt gtattcatat cagaagaact ttccggagag
     4861 ctgttggagg ggagatcact aagaatttcc gttcagccga cagggatagt ttgccgcagg
     4921 gaggagacat cagatttcat gaaagcggtg tggagaacgg gatcaatgcc cctacccccc
     4981 tatatcaaga ggaaaccgga ggagatggac aagcagtggt atcaaacggt atacgcggaa
     5041 cacgtcggtt ccattgctgc accaaccgct ggtctgcact ttacagacag cctcattgcc
     5101 gggcttgagg agaagggtgt cctgatcaga tttctcaccc tccatgtggg taccggcacc
     5161 ttcaggccga tacgggtgaa agacgttgaa gagcacccta tggatgcaga gtatttcgag
     5221 atagacaggt cccttatgga tacaatcgat aaggtaaagg gttccggaag gagggtcatt
     5281 gcagtgggaa caaccactac aagggccatt gaaggatttg ccagcaacag atggaaaccc
     5341 gccggagaaa acgggaattc gtcaggaagc ggatacatac gaggggtttc tgacatcttt
     5401 atctacccgg gctacacgtt caaggttgtg gactccctca tcaccaactt tcatcttccc
     5461 cgttccaccc ccctcttact cacctctgct ctctgcggag cgcggaacct tatgaatgca
     5521 taccactcag cgctctccat aggatataga tttttctcct atggagatgc tatgcttgtt
     5581 ttatgaagag ggatgcagca acgattgccg aattgctgcc gggcaagatc gtgcttattg
     5641 tcattacagc taccgcggta acgacagggt tcaccctggg ttattttgtg ggaagaagtg
     5701 tgtccttttc ccccgagtcg tctatgctca agcaatctcc cagcagcgat ctggcggcag
     5761 tgccgtcagc gacaaacccc tcatcagaac ttaagaatga agcctcatcc ccctctctgc
     5821 ctaagatgca tcctccctct aaggaaagca cacaggcttc cgtgcctccc tcactcggtg
     5881 gggcaattcc tttaaccgca gacgtgaggg atgaagagaa gataagcccg aaaagtcaaa
     5941 aggccgccat gacagaaaaa aaggctatcc ctgctgcagg agaacaatcc acttttccct
     6001 ccggggtttc gaatccggac atagagacaa ggaacgccct taacccacag agtacaccaa
     6061 aaagtagtcc ggcaccacac gcaggttcct cacacagcga agttgtgtat actgtccagg
     6121 cagctgcatt taagaaccaa aaggatgctg acaccttgag acaaaccctt gagggtaagg
     6181 ggtataaggt atttatcaag aaagaatctg gtccaaatgg agtggtcctg tttaaggtca
     6241 gggttggtga attcgaacaa aaaaaagatg cttcggtatt tgccttaaaa cttatgaaaa
     6301 cgggtgggtt gaatgccttt gccgtcttga aaaattaata aggagcaaat acggttacta
     6361 cccaaataga tctcgataca gaaaaggaac ttcccctcct ctatggtcct ctcactaaaa
     6421 accttaaact catagaagag tcactcggcg taaccataag tgcaagggga agtacgatat
     6481 tcattcaggg ggatgagccg tcagcccgga aggccggaaa tctgatcaat gaattgcggt
     6541 ccttgagtga agaggggtat accttgaacc ccgacgatat ccgttttgcg gtaagggcaa
     6601 tttcctctgg cggagaggta tccctcagag acctcatcat gaaccatatt cccgtgtcct
     6661 caaaaaaacg gtttataatt ccgaagacag aggtacaaag ggactacgtc gaagccatca
     6721 agagccatga tattgtgata ggcatagggc ctgcaggtac gggaaagacc tatctttcca
     6781 tggcaatggc gatcaatgca cttctcaaga aagaggtgag caggattgtc ctcgcgcgcc
     6841 ctgcggtgga agcaggggaa aggctcgggt ttctccccgg tgacatgtat gaaaaggtga
     6901 atccttttct cagacccctc tacgatgcac tctttgatat gatggaaccg gaaaaggtgg
     6961 gcaagctcat cgaacgcggt gtcatagaaa ttgcccccct cgcattcatg aggggcagaa
     7021 ccctcaatga ctcctttatt atccttgacg aagcgcagaa caccacgtcc gagcagatga
     7081 agatgtatct cacaagactc gggttcagct caaagaccgt gattacggga gataccacac
     7141 agatagacct gcctgcagga aaaagttcag ggctaattga ggtagaaaaa atccttgagg
     7201 gaatagacgg gattaaattc atctatttct ccgagaaaga tgtggtgcgc cacagactgg
     7261 ttcaggagat cgtaaaggca tatgaaaggc atgaaaaacg gagctcagat cattcaaaga
     7321 cccaggaatg agcaaataac cacggctagg aagtatacgc tcctcattgc ccttggcctg
     7381 ttcacttccc ttgccatcca gacaaagatc ggcattgaat ccttcatcgg tggctttctc
     7441 gtggcctctc tgctgctctt catcctctac agggatatcc tgagatataa gccccaatat
     7501 atgaaaaagt atcccatgct ctttctcctc gggttaatga tcatcaccac cattgtgctg
     7561 ggaagacttt ctcattatgt actgctcaac ctttccaagg ggttcggctc ccttgccatt
     7621 gaaggggcag tgtttggcat gcctatcgct gcgggagcca tgctcgttac ccttatgttt
     7681 gactttcaca ccgccatctt tttttccttt atcgtgagcc ttttgtcagg cctctggctg
     7741 ctggatgccg tctatcccgt atacgtattt gtagggagtc tcaccgccgc ctttagtgta
     7801 atcagatgca aaaaacgctc ggcctttctg aagggaggtc tctctgtggg aggggcaagc
     7861 atcgtcacct caatactcct cttgctttct cagggggaac tcttcacaga aaaggctatt
     7921 tccaccttaa tctttgccgc ctcaacaggt ctcagtgtgc ctgccatcgt ttccctgctc
     7981 cttccggtcc tcgaatacac ttttcatgtc actaccgaca taagccttct tgaactcctt
     8041 gacctcaatc agccccttat gaaaagcctt atgatcaacg cgcccggcac ctatcatcac
     8101 agtgtcattg tgggaaacct cgtagagtca gcagcagagg cagtgggcgt taacccgctc
     8161 cttgcgaggg taagtgccta ttaccatgac ataggaaaga tcaagatgcc tgaatatttt
     8221 gtcgagaatt atacgagcgc ggtgggcaag cacgacaggc tcacccccca tatgagcacc
     8281 atgatcattg gttcccacgt gaaggaaggg atagaactcg gctatcagca caagttgcct
     8341 cagtccatca tagatatcat tcaacaacac cacgggacaa gcctcatgac ctacttctac
     8401 cagaaggcaa aggaccacga tgtgggtgat catccgttgg tggaagatta taaataccag
     8461 gggccaaagc cccaaacgag gatcgcggcc cttgtgatga tggcagatgc ggtcgaggcg
     8521 tcctccaggg ttctcaatga tccgactcct gcacgcatca cggcactggt cgacagaata
     8581 attaaccaca tatttctcga aggtcagctg gacgaatgcg aactcaccct gaaagacata
     8641 tccgaaatca agaaacattt ctcgtatatc ctcaccggca ttctccataa gagaatcgat
     8701 tacccgggct ttgatttcaa ccaggaacca aaggaaattt tcaaagacag tgaaaggggg
     8761 ctcgggggcg acatgccgtt taccccccac actcatgaag atcctcatag agaaccgcca
     8821 aagtctcata aagataaacc ccaggaagct aaaaggcgac tcgctggcac ttctcacgat
     8881 attccgtctt aataaggcag aattgggggt actgcttgtg gacgacaggc agatgaaaca
     8941 gctcaattgc ctccatcgca gcctgaacaa gaccaccgat gtgctctctt tccccatata
     9001 tgagcggaga gttgaaattc ccgttgacag agagtcattg ttgggtgata tcgtcattaa
     9061 tctcgatgcg gcaaagaggc aatcatccct ctgcggcgtt accctctttg gtgaggtgag
     9121 gcgtctcctc atccatgggt tcctgcatct cttgggttac gaccacgaga gaagtctcta
     9181 tcagaaaaaa aagatgagac gacatgaaaa tgagctaagg aatgcccttg aaacgctgga
     9241 ttaagagtgc caactttgcc atagagggga tactccatgg aacaaagacc cagaggcatc
     9301 tcagatatca cctctattct gctgcaggtg tcctgatcct gtgttatatc cttgggatat
     9361 ccagaaatga cttcctcgtg atcgctgtca tggtgattgt tgtcctcgtt gccgaactct
     9421 ttaataccgc cattgagaca atggttgaca tcctttcccc gaaacatagc gaaaaggcaa
     9481 gaattgcaaa agatgtggct gcaggagcag tgctcaccac cgccttcggt gcagcggtca
     9541 tcggctacat tattctctcc ccctatatcc gcaagggatt tcaaacaggc ttcagtatcg
     9601 caaaacattc aggagaagat atctcagtta tggcggtcat cctggtactt atccttgtgg
     9661 tcatcaccaa ggcgtatctc ggaaagggaa ctcccttaag aggcggcatc cccagtggcc
     9721 attcagccct cgccttttcc ctatgggtgg gagtcagcta caccacggga aatgtacttg
     9781 cgtccgcatt ttgctttacc ctcgctgcag tcatcgctgc gggcagggtc gcttcaaaga
     9841 tccatacggt atgggaagta gttctcggag gggcaatcgg cgcctcgctt acttttcttc
     9901 tctttcagat attctcctaa aaccatccga atcacccttc cctcagcctt cactgtttgt
     9961 ctaaataaaa tacaacgttc accgttgcac ttaccgcaat atctcccgcc tctattgata
    10021 tctcaggagc tgcaacggcc attttcgtct ccatgaccgc ctgcctgtaa atgggatgtg
    10081 gttgtcttcc gcaggacgga gaaatacttt ttattcccga aattgtcgtg ccgagtgtcc
    10141 gggcaacaaa agctgcctct ctttttgccc cctctgctgc cttccttatc acaccatcgc
    10201 agtattcctt ttcttctgag agggtgaagc taataccctg agcctggttt gcaccaatct
    10261 gcatagcgtt gtctataatc tttcctgcca tctctatctt tttcgttctc accgtcacct
    10321 gattgagtac cttgtaacca gtaagaatac ttttcttttt cacctcatcg tattcataca
    10381 caggctggac tgtataggaa gaggtcttta tggaatcccc ggcggcaggg ctaatgaggc
    10441 gtttcaatgc acttaccatt ccttcagact ttttgctgtt ctcagccact gtctcggata
    10501 ccgttttccc tgctgtctca acaccaaggg ttataaaggc agtatcaggg gggtaatatt
    10561 cccttgcctc acccataaca gaaatggttc ccttctcttc tttcaattcc tgggcatacg
    10621 acatgcgaag gatggtgact acaacaagac atgcaaaagc acctgccatc gtgaccttac
    10681 ctgttctgtg tcgcattatg cgaactcctt tctaaagaag tcttcctaac tgttccagaa
    10741 gtctcttaca ttatactgcc agaagccagg ttattgtagt caccataaat tgtattggca
    10801 ctcgggttcc agtgtatact aaaagaatgg gtctgacatt ccttatcccc gcgctttgtg
    10861 tccttattgg ctattcttgt gcagatgcgt ttcaggcagg ccccctccct tcccggatta
    10921 gccccggcga tgcctttctc attaaggtat cagaggccag ggcgtcagaa attcctgctg
    10981 cctccctaaa cggaaaggaa attctcttta gccgttgcgg ggaaacatgt tttattggta
    11041 ttggcgccgc ggcattggaa acaaaaccag gccgccacgt gatttacctt cgtatgggag
    11101 aaaagagcac gcagctgtcc ttgctggtga aacgtacgcg cttccccaca ctctttctca
    11161 ccctgcccaa agaaaaggtg ttcctcagcc ctgaagacct ggaaagggct gaacgggaag
    11221 aagagcggtt acgattatta tggatgacca taagcgaccg gctgtgggaa ggaagttttt
    11281 tgctccccct ggaacatagc atctcatccg tattcggcac aaaaagaatg atgaacaggc
    11341 aagtggcgag catccataag ggagtggata tcaggggcaa agaaggagat gaagtgaagg
    11401 catccaacag ggggaaagtg gtttttgcag aagaactctt tttcggcggc aataccatta
    11461 tccttgacca tggtcaggga atattcacta tgtatatgca cctctcttca ttcagggtca
    11521 aacctgaaga tgttgtttca aagggtgaca tcattgggtc tgtaggctca tcagggagat
    11581 ccagcggacc tcatcttcac ttcggggtga aaatccaggg agtgagcgtg aatcctgttt
    11641 ccttcgtgaa gctcacgctg taagcggtat caactcttct ccattgtcat tgcttactta
    11701 gaattaagca cacatacaca tacccggcca taatatgatg tatgtcatgg tgtttactac
    11761 atgaaagctg taataattca tcatgaataa atactgcggt ctttgtgcga tagtgtgtgt
    11821 aatggcagtg attttatggt attcctgctc ctttgcctgt aacgtctgcc atagcaagag
    11881 tccccgaatg gtgaagatgc acacagagct tggctacaag gattgttttg cctgccatac
    11941 gccgggcaac aggaatcatc tgaggaatgg agactgcaaa ggaaaagcga cgagaagtgc
    12001 ataaggtgtc ataagaaata atattcaaac agggaaccat ggttcatgct ctctttctcc
    12061 actttcgcgg catccagcac ttttccctct tttgttcctt tctaaggcta cagcgtgtga
    12121 cgtaatgcgg tgtttatcat ttcttgattt catctatttt gtcgaaacaa tttcttaacg
    12181 caacaagcat ttcgtgggat aacctctgaa tcatatccat cctttcaatc gccttaagca
    12241 agagctcatg cagatcgtcc tcggtaaccg atgcatccat aagctctaat gtgcccttaa
    12301 ccgctaatat gttctcgcca agctgatttg acagttcatc gagtttatta tttctttctc
    12361 ccataggttt tctccttttt tatagtttaa attcggtgaa atcaaaacca ctactccctt
    12421 aagtccttta acaacctctg gaattcccgg gattctgcaa gtatccgatg ggctgcgtca
    12481 cgaagtttgt ccacctcttc aggcgacagc ttgaatgagg tgggaagccg cataaaatac
    12541 aagcgttcct ccccgtcctt aagggcatca aattttacct caatgacata gaactggatg
    12601 tcgccgcagg aacctggtct tgtggaaatc ttgcccttcg gacatcttcc cgtacgaacc
    12661 tggtctgccc atcctttcac gctttccttc aataacgcca acgtctccac gttataccgt
    12721 tctatagcta tggatgaata ggattcgagc attgcggcaa atgcaggagg gctctcaacc
    12781 ctgtcccaga aggaatccgg ctgcgtttct gcattcacca caataaagat gaccttatga
    12841 aggttctcat ggtgagtgcc cttgatggtc ttccagaaat ccccccgata aattattctg
    12901 tcaaggatcg ccctcagacc gagattgtca gcaactccgc cgtccaggag atggagaaac
    12961 ggtttcttct tggaatccag gtagggagcg atattgttcg caagataaaa ctggcgctct
    13021 gatatttggc tctctttaaa gaccctttcg atgggtcccg gcatcctgta attgcagctt
    13081 cccgcataat tgcgcacggt aatgggtgtg agcacgatgg gaaccgcgga tgacgcagcc
    13141 acagcccttg ccacagaaaa tcgtgacaca tccgagcaga tggcgtcaaa catgtcctgg
    13201 gtaaacccta ctcttatgcc ataggtcatg tcagtggcat tgatgatgac catgggccct
    13261 ttcctggctg caatgtcgcc aaacgtaccg ccttcaaaaa tgtacttgtc ataatattct
    13321 gctgccagat cgctacggtc aaaagatgga gaaaatagcc tgacccagtt cacagggttg
    13381 aagaatgtcc tcgatacgag ttctccctga acattcttct tcagaaacct ttgctcaaag
    13441 tcctcaaaaa tacgttcccc aaaaaggcca taataccccg cggtgaaact tccgcccgat
    13501 acgctggata tggtgtccac ttcatcaaga agccttcgct ttctcccatt gatgctaatc
    13561 tccctgactg ctagttcttc aagcacaccg taagaaaatg cagcggcacg ggttcctcct
    13621 cccgagaagg tgacaaaaaa gagtatatca tcggatcgtt cggggtccct cacattcttg
    13681 gccctgtagc cggcttcggg ggcatactct ttgaggggct cattgacagg atagtgtgcg
    13741 cagccgctta tagaaaagac aagcagtata cttactgaca gcaaataact tctgtatgaa
    13801 tgcgtgggtt gtttcctgca gacaggattc aaatcgttta tgacttctcc actgggttcc
    13861 atttgtacac tcatcacccc at
//
LOCUS       DATKOU010000128         4985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4786160, whole genome shotgun sequence.
ACCESSION   DATKOU010000128 DATKOU010000000
VERSION     DATKOU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4786160"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..989)
                     /locus_tag="VMU21_05940"
     CDS             complement(150..989)
                     /locus_tag="VMU21_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HUO77102.1"
                     /translation="MERLGNKIETKFAVKIAKILRTKRMTEQEKLFEIALEGGESLDY
                     EPGQFIMITVFGAGEIPISLTSAPTSRLSFEICVRAVGRVTNALHTLEAGDLVGIRGP
                     YGKGFPIRILEGNDLLIVAGGLGIAPLRSLINYVLENRRDFGKVHILLGCKSPKDMLF
                     VDELNHWDKRMDVHYECTVDKGAPDWAGNVGVITTLIPGVDLEPERTFAVVVGPPVMY
                     KYVIRELLAKNIPERQILVSFERHMKCGIGKCGRCQIQNLYVCQDGPVFNYDDIKHLS
                     EAL"
     gene            complement(965..2044)
                     /locus_tag="VMU21_05945"
     CDS             complement(965..2044)
                     /locus_tag="VMU21_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO77103.1"
                     /translation="MKDMQHQYALLSKDNLDGFISRLSGIKKVMAPFSKGHNNYSFEE
                     ARSGEGIALNYIPTILPPKKFFMPQYETLFSYKVGKTINVETVVDYEEMVLFGVHTCD
                     LAGIQCLNMVFSERPKDYNYLTRKPNIFVIGLECNNYCDEHASCHLVNASLPSGGYDL
                     FFTDLGDYFMVHVNTQEGEEMVEATKSFEPAGKDQLHELADLREQKRKIFKNEVTISP
                     RHLPELFEGAFESKVWNELGERCLACGNCTNVCPTCYCFDIREDVSLDRKSGSRYRMW
                     YSCQVEPFAKVAGGINFRGERSARQRHRYYRKFKYPVLKFSRFFCTGCGRCSRTCMAG
                     IHLKETLNALVKEKQSKLWRDWGIK"
     gene            2575..3120
                     /locus_tag="VMU21_05950"
     CDS             2575..3120
                     /locus_tag="VMU21_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUO77104.1"
                     /translation="MDEDIQLVKRYLGGDAEAVETLVVKYQKRIYTIVYRMTRNVEDA
                     KDLTQKTFVKAIEGIKGFRKESSFKTWIYRIAINTGLTHLKGNRREERELDESMMGSH
                     PGTLSTIIENEKRGFIRRGLDELPERQRLAVILRAYEGLSCTETAQVMGCSEGAVKAH
                     YHHGVKRLKEIVKEKGYEIRT"
     gene            3104..3673
                     /locus_tag="VMU21_05955"
     CDS             3104..3673
                     /locus_tag="VMU21_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HUO77105.1"
                     /translation="MRSEHEEIMEMLPEYLKGSLPGELGNEVQAHLEVCKECKSEVSF
                     LREIVDSEVPDPGDLFWQSLPRRVRLTVEKKKERFPLGFLVRRLPVAAAIAAALLLTL
                     IYIYPNKDKVSVQDPFFNDPLTASVSDYTDATEEDIPLIAEEITVDESYLPRDNYMDP
                     SYHNEFASLSSGEMESLYEALKGEQQSGG"
     gene            3833..4774
                     /gene="cysK"
                     /locus_tag="VMU21_05960"
     CDS             3833..4774
                     /gene="cysK"
                     /locus_tag="VMU21_05960"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HUO77106.1"
                     /translation="MKPHKDILKLIGNTPLVEITRLSHGDAAEIWAKLEGFNPGGSVK
                     DRIALSMIETAEKEGRLEPGGTIIEPTSGNTGIGLAMVAAVKGYRLILTMSERMSVER
                     RNILQAYGAELILTPGERGMLGAVERAEEIYKKNPGYFMPLQFENPANPGAHRKTTAM
                     EIIHTLGGVPDAFVAGVGTGGTITGIGEVLRERRSDVLIVAVEPAGSAILSGGEPGPH
                     KIAGIGAGFYPGVLNTRIYDEIIPASDEDAMETTRQLALREGILAGISSGAAMWAAMK
                     VAERLGKGKKVVVIFPDRGDRYLSTGLFTPETVDACR"
BASE COUNT         1231 a         1215 c         1275 g         1264 t
ORIGIN      
        1 cttatcccat ttctcggaca tcacctcgcg gaattccacc acgtcaagaa agttgagaac
       61 atcgagaaga gactcgccga gatttgccac ctgcagctgg cagccctcgc agcaggcaaa
      121 gtcaaaaaaa gcagtcttag gttttatcat cacagagcct cgcttaagtg tttgatatcg
      181 tcataattga atacgggacc atcctggcaa acatagagat tctgtatctg acagcggcca
      241 cacttgccta tgccacactt catgtgcctc tcaaaggaga ccagtatctg acgttccggg
      301 atattcttgg ccaggagttc ccttatcacg tatttgtaca ttaccggcgg gcccacaacc
      361 acggcaaagg tcctttctgg ttccaggtct accccgggga ttagtgtggt gatgactccc
      421 acgttgcctg cccagtccgg ggcaccttta tcaacagtgc attcgtagtg gacatccatt
      481 cgtttgtccc aatgattgag ttcgtcaaca aaaagcatat cctttggaga tttgcagcca
      541 agcagtatgt gtaccttgcc aaaatcgcgg cggttttcaa gaacataatt gatgagagag
      601 cgcagaggcg ctatccccag tccgccggcc acgatcagca ggtcgttgcc ctcgagtatt
      661 cttatgggga agcccttgcc gtacggcccc cggatgccta ccaggtctcc tgcctccagc
      721 gtatggaggg cattagttac ccttcctact gctctcacgc agatttcgaa gctaagacgg
      781 ctcgtggggg ccgacgtaag agatatcggt atctcgccgg cgccaaagac agtgatcata
      841 ataaactgac cgggctcgta gtcgagactc tccccgccct ccaacgcgat ctcgaagagc
      901 ttctcctgct cggtcatcct cttggtcctg aggatttttg ctatcttgac agcaaacttc
      961 gtctctattt tattccccaa tctctccata gcttcgattg cttctccttt accaaggcgt
     1021 taagggtttc tttaagatgt atgccggcca tgcacgttct gctgcacctg ccgcatccgg
     1081 tgcagaagaa tctcgaaaac ttcagtacgg gatatttgaa tttacgataa taccggtgcc
     1141 tctggcgcgc ggatcgttcc ccccggaagt tgatgcctcc ggccaccttt gcgaagggct
     1201 ctacctggca ggaataccac atcctgtatc tgctgccgga ctttcggtcg aggctcacat
     1261 cctcacggat atcgaagcag taacatgtgg ggcagacatt agtgcagttt ccgcaggcca
     1321 gacacctttc gccgagttca ttccaaacct tgctctcaaa ggcgccttcg aagagctctg
     1381 gaagatggcg cggagatatc gtgacctcgt tcttgaaaat ctttctcttc tgttcccgca
     1441 gatcagctag ctcgtgtaat tggtccttcc ccgcaggttc aaaggacttt gtcgcctcga
     1501 ccatctcctc tccttcttgg gtgttgacat ggaccataaa gtaatcacca aggtcagtaa
     1561 aaaaaagatc gtatcctccg ctgggaaggc tggcattcac caggtgacag ctcgcatgtt
     1621 cgtcgcagta gttgttgcat tccagaccga tgacgaatat gttgggtttc cttgtcaggt
     1681 agttgtaatc cttaggccgc tccgagaata ccatgttaag gcactgaatg cctgcaaggt
     1741 cgcatgtgtg gaccccgaag agtaccatct cttcgtagtc cacaacggtc tcgacattaa
     1801 tcgtcttgcc aaccttgtat gaaaacagcg tctcgtactg aggcataaag aatttcttgg
     1861 gaggaagtat tgtagggata taattcagcg cgattccttc gcccgacctc gcttcttcaa
     1921 atgaataatt gttgtgaccc tttgagaaag gcgccatgac cttcttgata ccggacaacc
     1981 ttgatatgaa cccgtcaagg ttgtccttgc tgagcagagc atattgatgc tgcatatctt
     2041 tcattggttc ctctttttag tcttaataaa catttgttta tacctggatc cagtgataaa
     2101 cgtcttcaca ccctggctct cttcatagcg tgcatcgggt tatcacgcct ccttccatat
     2161 caaatcctgc cataccaaag ttatcacgtc gtcagcgcgg ccatcttatt gttaagaccc
     2221 gtcgctctcc ctccttctta cggaggttta gctttgtcga aatccgctct ttgtgttact
     2281 ttacttttat catggataga ggcaactgtc aacttataat tttaggtctg tcaaggtcaa
     2341 tgtatggact ccgaaaagta acatcttttc atagctgaca gaggcatatt ccttccctta
     2401 aacgaaattt tgtgagaagc gttcagtata taactaataa gtcttctcta taatttcacg
     2461 ttgcccagtt tgtggtgcat tgtcaagcac ttgagcgcct tccatgaggg gtatataatg
     2521 gaaagacatc ttttgtgcta aacctttttc tgttgaccct gtctaatgaa acagatggat
     2581 gaagatatac agcttgtgaa gagatacctc gggggggatg cggaagctgt cgagacgctt
     2641 gtcgtgaaat accagaagcg gatttatacc attgtgtaca ggatgacaag gaatgttgag
     2701 gatgccaagg acctgaccca gaagactttt gtcaaggcta ttgagggaat caaagggttt
     2761 cgcaaggaat cttcattcaa gacatggatc taccgtatcg ccataaacac gggtctcacc
     2821 catcttaagg ggaaccggcg tgaagagagg gaactggacg aatcaatgat gggcagccac
     2881 ccgggcacgt tgtccacaat tatagaaaat gaaaagcggg gcttcataag gaggggattg
     2941 gatgagctcc ccgaaaggca gaggcttgcg gtcatcctca gggcatatga ggggctgagc
     3001 tgtactgaga ccgcccaggt catgggatgt tcggaaggcg cggtcaaggc gcattatcac
     3061 cacggagtga agcgattaaa ggagattgta aaggagaagg gctatgagat cagaacatga
     3121 agaaataatg gagatgctcc ctgaatatct gaaaggctct cttcccgggg aactgggaaa
     3181 cgaggttcag gcccatctgg aggtgtgcaa ggagtgcaaa agcgaggtct cctttctccg
     3241 tgaaattgtt gacagtgaag tgcctgaccc cggggacctc ttctggcaga gccttccccg
     3301 gagagtgaga ctgaccgtcg aaaagaagaa ggagagattc ccattaggat tcctggtacg
     3361 acggctgcct gttgcagcag caattgccgc ggcattactg ctgacgctca tctatatata
     3421 tcccaataag gataaggtgt cggtgcagga ccctttcttt aatgatccat tgaccgcttc
     3481 tgtgtcggat tacactgatg cgactgaaga agatatccct ctcattgctg aggaaatcac
     3541 agttgacgag tcgtatcttc cccgcgacaa ttacatggac ccttcctatc acaacgagtt
     3601 tgcgtcatta agttccggag agatggaaag tctctatgag gcgttgaaag gggagcagca
     3661 atcagggggc tgatcagggt cagttcaaca tgacaggttt cattgttgtg taggtctttc
     3721 gatgtctctg cttcactccc tatgatataa tcactactct acctgtcgcg taattatttc
     3781 cttggggaaa agagcgaatt ataagattga acagagcttt ccgggagggt ttttgaagcc
     3841 ccataaggac attttgaaac tgatcggtaa cactccgctg gtggagatca cccgcctttc
     3901 tcatggagat gctgcagaga tatgggcgaa gcttgagggt tttaatcccg gaggttctgt
     3961 aaaggaccgg atagctctct ctatgataga gactgccgag aaggaaggga gactggaacc
     4021 cggcgggacg atcatagagc ccacgagcgg aaatacaggg atcggccttg ccatggtcgc
     4081 ggcagtgaag ggataccggc tgatcctcac catgtccgaa aggatgtctg tggaaaggag
     4141 aaatatcctt caggcgtatg gcgcggagct tatcctcaca cccggggaga gaggaatgct
     4201 gggcgcagtg gaaagggcag aggagattta caaaaagaac cccggttact tcatgccttt
     4261 acagttcgag aaccccgcaa acccgggtgc acatagaaag accactgcaa tggaaatcat
     4321 tcataccctg ggcggcgtcc cggatgcgtt tgttgccggt gtagggacag gagggacaat
     4381 aaccggcata ggagaggtct tgagggaaag gcgttctgat gtcctcatag ttgctgttga
     4441 gccggctggt tctgccatcc tctcaggcgg cgaaccaggc cctcacaaaa tcgcgggcat
     4501 cggtgccggt ttttatcccg gagtgctgaa cacaaggata tacgacgaga taatccctgc
     4561 gagcgatgaa gatgcgatgg agaccacaag gcagctggca ctcagggaag gaattcttgc
     4621 aggtatctcc tcaggtgcgg ccatgtgggc agcaatgaag gtcgcagagc ggctggggaa
     4681 aggtaaaaag gttgtggtga tcttccctga caggggagac cgctatctga gtacagggct
     4741 gtttactcca gagacagtgg atgcttgtag gtagaagttc agaccgacta acgttgtacg
     4801 tttccgccgt gtgcctgctg gaccatcaag aaaaagagtg atgtatgcct cattctcccc
     4861 tgccataacc taaatccatc catattctcc ctattgcgtg cggtatttct gctaacttgt
     4921 tcatatccgc aattgccaga catttatctc gcatatggct caaatttggc tcgataggct
     4981 aggcg
//
LOCUS       DATKOU010000129         5460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3246599, whole genome shotgun sequence.
ACCESSION   DATKOU010000129 DATKOU010000000
VERSION     DATKOU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246599"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VMU21_05965"
     CDS             complement(<1..502)
                     /locus_tag="VMU21_05965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO77107.1"
                     /translation="MSEFKKFDPRKLDKLNDPKRLEYLNPDLIWEKAALKDPSILIDI
                     GAGTGFFALLFSKKMKKGKVYACDISDEMLLWLEDNLPSESKGRVIPVRMEESSVPLS
                     EDMADLVYMINLHHELEQPQKILREALRLLRRGGKLLIIDWKKEQTPEGPPLEIRVTE
                     EAIESQM"
     gene            complement(771..2810)
                     /locus_tag="VMU21_05970"
     CDS             complement(771..2810)
                     /locus_tag="VMU21_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77108.1"
                     /translation="MKVRHSLRGYVVIVGIFAAIILALVTLNIYFQRSLKMEMAEQFN
                     HQQLLLAKTEALRIESYLSVIKEDMLHVAQFAAVMNFPKEADFRLLTDAVCRHIGDIK
                     RRVAFLDSEGRIRFTRGTMAIQELDDKYFAKAVKSGCQDDILIEQGLKRLRLTAPVCR
                     SGSLTGALMVFIDLQDIATMFLGPIKSGSRGYAWMMDNKGTLLYHPTQADMMGRNLYQ
                     TDSSCFKCHKSFDVEKKIIEGSGDFHGRYVAPSGEDKILAFSTSSVGSAKWIVAVSAP
                     YTEVTKLIGKSMKVYSLFVMFIVLTTGFTSVILIVFFRKKAKAEELEHEVDIRTKELS
                     TEKEKLHTVVSALGSGLILLDAKGKIQWTNQAMKDIAGRDVSGKSCEEICSDYTVIGS
                     YEEHGLQTEIISNLFGKSDRYYQMTTAPVKGENGSIDGYIKLLQDVTEMKRTEEQLIH
                     SEKLSALERLTSGIAQEIGNPLTSVFCFVQMLREVEHDELKKETIETIYSHMNRIADI
                     LKQLSSFSKMPPPELKPVRVNNLIEDSLALMNYDKRVQDITIMKDLSPALPEIATDGN
                     QLRQAFVNVILNAADAMPDGGTLTIRSRRENDSVVIDFEDTGPGVTPEDLSRIFDPLY
                     TTEEKGTGLGLAVSYRIIKKLNGNMTAESEPDKGCRFVITLPVRKADKGASHSRK"
     gene            complement(2807..4135)
                     /locus_tag="VMU21_05975"
     CDS             complement(2807..4135)
                     /locus_tag="VMU21_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUO77109.1"
                     /translation="MKNPQFLPEVFETLRDGLMVVDNKGNIVLFNRAAEEITGYRKED
                     VIGGESSIFNCEMEPNKAGAEADSQLKEFGATYNMECRIRSGDGRMVHLLKNVTTLKD
                     EHGEIIGTVESMTDITSLYKKEMELQGLKQELRQEYWFMGLLGKSVPMQRLYEHIRNA
                     AMSEAPVLIYGESGAGKNLVAHALHKLSRRKDGPFMQMNCAALNEQLLESELFGHKKG
                     AFTGAVSDRTGRFEAAHNGSVFLDEIGDMPMSMQVKLLRVLEEKVVERVGDHKPIPVN
                     IRLISATNKDLTNLIQSGKFREDLLYRINSITIKVPSLRERSEDIPLIAFHYLKKISS
                     VNNKEIKSISPPAMEILINFPWPGNVRRLINALEHSVVTCKTDTIDVSDLPDYLVYEK
                     KADGGESRIDPEKIRATLALYKGNKTLAAKNLGISRVTLWKKLKTLGIEQ"
     gene            4381..4950
                     /locus_tag="VMU21_05980"
     CDS             4381..4950
                     /locus_tag="VMU21_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77110.1"
                     /translation="MNKVLIAVDGTKASTAVLSSFYNLVKKPEQVILLHVQKLQGRSL
                     MLDMLGDAEMSTLKESIAGTEHKDALDEKADKILNYYKGELQDSGTVSITTEIRAGHP
                     CDEILKVADEENVDLILLGNSGRRGLNRLISGSVAREVQKHTKVPVMVAKRPSTCEEP
                     YSWKDAIASVSITSAIVLALFIFGTFVGR"
     gene            4986..>5460
                     /locus_tag="VMU21_05985"
     CDS             4986..>5460
                     /locus_tag="VMU21_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77111.1"
                     /translation="MKKVLVVVDDTKGSKAVLSVFKNLVRPPQEVLLLHVQRLEGKSL
                     MIDMLGDAELSTLRESLKGTEYKEKLDRESEQILKYYKREFEDGGLISVRTLVRDGIA
                     ADEILKVAEDEKVDLIVTGANGNKGMLRLVAGCVPSEVERRSKIPVLVGKEEASTE"
BASE COUNT         1346 a         1300 c         1259 g         1555 t
ORIGIN      
        1 gcatttggga ctcaatggcc tcttccgtga cccttatctc aaggggagga ccttcagggg
       61 tctgctcttt cttccaatcg ataatcagaa gtttgccccc tctcctcaaa agtctaagcg
      121 cttccctgag gatcttctga ggctgctcaa gttcatgatg caggtttatc atataaacca
      181 ggtctgccat atcttcggaa aggggcactg aactctcctc catcctcaca ggaataaccc
      241 tgcccttaga ttctgaggga aggttatcct ccagccataa aagcatttca tcagagatat
      301 cacaggcata tacctttcct ttcttcatct tcttgctgaa aaggagagca aaaaagccgg
      361 tgcctgcccc tatgtcaatc aatattgagg gatctttaag ggctgccttt tcccatataa
      421 gatcgggatt caggtactcc agccttttgg gatcgtttag cttgtcaagt tttctcgggt
      481 cgaatttctt gaactcgctc actaatcctt accccgtttc tccctcataa cccagccaga
      541 agatacgaaa gaccgagttc atacgatcag gtattgagtg tttttctgag gaagcttcat
      601 gcggaagaag ttcagagaca tggtcgagac tggaccctga aaggtgccgg ccctgtaaag
      661 cgcggatatt ctatgaatgc gcacagatgc cgggagcatg attccccatt catacagatt
      721 gagtcagtca gaatttaata agactatttc ttttttgctg agcatggtcg ttacttccgg
      781 ctgtgagaag ctcctttgtc tgccttcctt accggaagcg ttatcacaaa tctgcagccc
      841 ttgtcaggtt cgctttctgc agtcatgttt ccattaagct tcttaatgat gcgatagctc
      901 actgcaagtc cgaggcccgt gcccttttcc tctgtggtgt aaagcggatc gaatatcctg
      961 ctaagatctt cgggggtgac gccgggcccg gtatcctcga aatcgatgac aacactatca
     1021 ttttcccttc tgcttcgtat ggtgagtgtc cccccatccg gcattgcgtc agcggcattc
     1081 agtattacat tcacaaaagc ctggcggagc tggttaccgt cggtggcgat ttccggtaga
     1141 gcgggcgaga ggtctttcat gatggtgatg tcctgcaccc gtttgtcata attcatgagt
     1201 gccagagaat cctctataag gttgttcact cgtacgggtt tgagctcagg cggcggcatc
     1261 tttgaaaaac tggaaagttg tttcaggatg tccgcaattc tgttcatatg ggaatatatg
     1321 gtctcgatgg tctctttttt cagctcgtca tgctctactt ccctcagcat ctgtacaaag
     1381 cagaaaactg atgttaaagg atttcctatt tcctgggcta tgccagaagt cagtcgctca
     1441 agggctgaca gtttttcgga gtgaatcaac tgctcttcgg tccttttcat ctctgtcaca
     1501 tcctgcagca gcttgatata tccgtctatt gagccgttct cgcccttcac aggagccgtg
     1561 gtcatctgat aatatctgtc gctctttcca aaaagattcg atatgatttc cgtctgtaac
     1621 ccatgttctt cgtacgagcc gatgaccgtg tagtcggagc agatttcctc gcaagacttc
     1681 cctgaaacat ccctgccggc aatatccttc atggcctgat ttgtccattg tatcttgccc
     1741 ttggcgtcga ggaggataag gccgcttccc agggcgctta cgacggtgtg gagcttttct
     1801 ttctctgtcg agagttcttt tgttctgata tcgacctcat gctcgagttc ttcagctttt
     1861 gctttttttc ggaaaaagac gatcagaatt acagacgtga accccgtggt caggacgatg
     1921 aacatgacaa acagcgagta aaccttcatg gatttcccta tgagcttggt gacctcggta
     1981 tagggagccg acacggccac aatccacttt gcacttccga cagacgaggt ggaaaaggca
     2041 agaatcttgt cttcccctga tggtgccacg taccttccat gaaagtcccc gctcccctcg
     2101 atgatcttct tttccacatc aaaagacttg tgacacttga agcaggaaga atctgtttgg
     2161 taaagattcc tgcccatcat atctgcctgc gtgggatggt aaagcagggt ccccttatta
     2221 tccatcatcc atgcatatcc ccgtgaaccg gattttatgg gaccgaggaa catcgtggca
     2281 atgtcctgca aatcgatgaa aaccatgagc gcaccggtga gggagccgga acgacacaca
     2341 ggcgcggtga gacgcagcct cttcaaacct tgctctatca gtatgtcatc ctggcaaccg
     2401 cttttcaccg cttttgcgaa atacttgtca tccaattctt gaatcgccat ggttccccgg
     2461 gtgaagcgta tcctcccttc actatccagg aacgcaaccc gcctttttat gtcaccgata
     2521 tgcctgcaca cggcgtcagt aagcaagcga aaatctgctt ccttgggaaa gttcatcacc
     2581 gccgcgaact gcgcgacatg aagcatatcc tcttttatca cgctcagata gctctcaatg
     2641 cgtaatgctt ctgtttttgc gaggaggagt tgctggtggt tgaattgttc ggccatttcc
     2701 attttaagtg accgctgaaa atagatgttc aaggtcacga gtgccagaat aatagcagca
     2761 aatatcccca caataacaac ataccctctt agcgaatgtc tcaccttcat tgttctatcc
     2821 ccagcgtctt cagttttttc cagagcgtaa ccctgctgat accgagattt tttgccgcaa
     2881 gtgttttatt gcccttatag agcgcaagtg ttgcacggat tttctcaggg tcaatgcggc
     2941 tttcgccccc gtctgctttc ttctcataca caagatagtc cggcagatcc gaaacatcaa
     3001 tcgtatcggt cttgcaggta acgacgctat gctcaagggc attgataagc ctgcgcacat
     3061 tgcccggcca tgggaaattt attaagattt ccatggccgg agggctgata ctcttgatct
     3121 ctttgttgtt taccgaagag atctttttca ggtagtggaa ggcaatgaga gggatatcct
     3181 cacttctttc tctcaacgaa ggcaccttga tggtaatgga atttatccgg taaagaagat
     3241 cttccctgaa tttccctgat tgtatcaggt ttgtaaggtc cttgttggtg gccgatatga
     3301 gcctgatgtt aaccgggatt ggcttatggt ctcccacgcg ctccaccact ttttcttcaa
     3361 gaaccctcag gagctttacc tgcattgaca taggcatgtc accgatttca tcaaggaaaa
     3421 cactcccatt atgtgccgcc tcgaatcttc ctgtccggtc gctcaccgcg ccggtgaacg
     3481 ctcccttctt atggccaaaa agctcgcttt ccaggagctg ctcattcaga gccgcacagt
     3541 tcatctgcat gaaggggcca tccttgcgtc tgctcagctt gtgtaatgca tgggcaacaa
     3601 ggtttttccc ggccccgctt tcaccgtata tcaggacagg ggcctcgctc atagcggcat
     3661 tacgaatatg ctcatataat ctctgcatgg gtacgctctt gcccagtagg cccataaacc
     3721 agtattcctg tctgagttcc tgcttgaggc cctgcagctc catctccttt ttatagagag
     3781 acgttatatc agtcattgac tcgacagttc ctattatctc gccatgttcg tcttttaggg
     3841 tagtgacatt ctttaaaaga tgcaccatcc gaccatcccc tgaacgtatt cggcattcca
     3901 tgttgtaggt ggccccgaat tctttgagtt gtgagtccgc ctcagctcct gccttgtttg
     3961 gctccatttc acaattaaat atggagcttt ccccccctat gacgtcttct tttcggtaac
     4021 cggttatttc ttcagctgcg cgattaaata atacgatatt gcctttatta tcaacaacca
     4081 tgagaccgtc cctcaaggtt tcaaacactt caggcaaaaa ctgagggttt ttcatcaatt
     4141 tgtctaacat attgtctaac ataattattt agatatttct agaattgttt gtccagcgtt
     4201 aatattgtta aagtttgcta ttaacaaagt taacacatta acgctcattt ttcaataaga
     4261 taatgattca ttttgtataa cttgttgata tacaaagata acttcattta ttgtagatat
     4321 ggcatatgat ttgcatttac aatgattagc tgcaaattga agcaaaggag gtaagcaaaa
     4381 atgaataagg ttttaatagc agtcgatggt accaaggcct caactgccgt gttatcaagt
     4441 ttttacaact tagtaaagaa gcctgaacag gtcattttac ttcatgttca gaagttgcag
     4501 ggaaggtcgt tgatgctaga catgttaggc gatgctgaaa tgtctacatt aaaagagtct
     4561 atagcgggta cggaacataa ggatgccctg gatgaaaaag ctgataaaat cctgaattac
     4621 tataaaggag agttacagga cagcggcaca gtcagcatta ccaccgaaat acgggctggc
     4681 catccctgcg atgaaatact gaaggttgcg gatgaagaaa acgtagacct tatcctcctt
     4741 ggtaatagcg ggaggagggg gcttaacaga ctgatctccg gctctgtagc tcgtgaggta
     4801 cagaaacaca ccaaagttcc cgtgatggtt gcaaaaagac catcgacctg tgaagaacca
     4861 tatagttgga aggatgctat cgcatctgtg tctatcacaa gcgcaatcgt tcttgctttg
     4921 tttatctttg gaacgtttgt gggaaggtaa ccatagcttt atttcttaat aaaaaaggag
     4981 gagatatgaa aaaggtattg gtagtagtag acgacacaaa gggttcaaag gcagtattgt
     5041 cggtctttaa gaatttggtt cggccacctc aagaagtgtt gctccttcat gtgcagcggc
     5101 tggaagggaa gtccttaatg attgatatgc tcggagatgc tgagctctca acgctgaggg
     5161 agtctctcaa aggcactgag tacaaggaaa agcttgacag ggaatcggag cagatcctca
     5221 agtactataa gagggaattt gaagatggcg gcttaatcag cgtaaggact ttggtcaggg
     5281 acggcatcgc ggccgacgag atcctgaagg ttgccgagga tgagaaggtt gatctcattg
     5341 taaccggcgc aaatgggaat aagggaatgt tgcggcttgt tgcagggtgc gtgcccagcg
     5401 aagtggagag aaggtccaag attcctgttc ttgtcggcaa ggaagaggcc tcgactgaag
//
LOCUS       DATKOU010000130         4982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5170126, whole genome shotgun sequence.
ACCESSION   DATKOU010000130 DATKOU010000000
VERSION     DATKOU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5170126"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VMU21_05990"
     CDS             <1..701
                     /locus_tag="VMU21_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77112.1"
                     /translation="TGSFFNPHYLGTGAMLWGANAYEYAGKSYSVNAAHQSANCPTCH
                     MANSTADNKNGGHTWAPNVVTCNTADCHGGSLGPVAAKSGSASPDVDTYRASSDTNNY
                     TGEAGGETLSIAQSIQSLEKKVIALLQARGIFYDDTLYPYFFADAGHTQQFTAWTPAT
                     LKAAFNLSFVVKGLPSAATSQVLVPNASAAVHNYKYCIQLLLDAYADLSGASLAGAVR
                     PAGSRPATGYGPGQ"
     gene            complement(762..1382)
                     /locus_tag="VMU21_05995"
     CDS             complement(762..1382)
                     /locus_tag="VMU21_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HUO77113.1"
                     /translation="MSSETVSPSNETLKTINHRRSVRTFTKQPVPDELIHVLLRSANE
                     APSAHNQQSWRFIVLRGEKKQELAQLINQRSGDFPRPASVLLRMAAKSIAGAPVVIAI
                     ANTGDLISHGTELFKVEKEMAYDFFRTMEIQGSAAAAQNLLLAATSLGLGTVWLGILY
                     LIKDDLLPFFGEPGGEFMAVVPVGYAAHPSSGPKKQPLEMKLKYLD"
     gene            complement(1569..1979)
                     /locus_tag="VMU21_06000"
     CDS             complement(1569..1979)
                     /locus_tag="VMU21_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77114.1"
                     /translation="MDRFVKNFILMSIIYLAVASLLGILMLGNPSLMHLKFVHSHLNM
                     LGWVSMMIFGVGYHILPRFAGKPLKSPKMGELQFWLANIGLVGMLFFYTLGSFHPEAG
                     IYKGLTVAFGLMEAGSIFLFFYNMIVTIYAKSEA"
     gene            complement(2070..2759)
                     /locus_tag="VMU21_06005"
     CDS             complement(2070..2759)
                     /locus_tag="VMU21_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HUO77115.1"
                     /translation="MRGYILLEGGSEFGGQMAEPDKRAIELAGGSDVQVSIVPTAAFP
                     DRNHQRAGQSGVRWFERLGAKQVTLLPLIDQASANQFSVASALRQSRLIYLLGGFPHY
                     LAQTLAGSLSWQAMREAYDTGAVIGGSSAGAMILCQHYYDPDTQRIREGLNLIPQACL
                     VPHHNTFGKGWASSLVPSIPYDVIVGVDEQTGLIDNGVKGEWTVLGKGVVTIYKGGKT
                     MTYRSGETFSL"
     gene            2882..3757
                     /locus_tag="VMU21_06010"
     CDS             2882..3757
                     /locus_tag="VMU21_06010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUO77116.1"
                     /translation="MVEDWLANAYEGMSRRQFLARLSAAGVGLAGFAIASSSVGGEVI
                     TTSGDGLAVADSTVLSGDFPVPIYGARPAASGKYPILVVIPEIFGMHEHIKDVTRRFA
                     KEGFFSITFEPYAREGGVLNLSDITAVRAVADSVPDKRVMEDLDAIVAYAKQHPSARA
                     DRIGVTGFCRGGMYTLLYSAHNRELRASVAWYGQLSPEKKPGIRTVGPLDIAAQIAPP
                     VLGLYGEDDLGIPVADVRKMEANLKAAHKTTEFVLYPGAPHAFFADYRPSYRSDAAKD
                     AWSRCLAWFNKYLKG"
     gene            4199..4660
                     /locus_tag="VMU21_06015"
     CDS             4199..4660
                     /locus_tag="VMU21_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77117.1"
                     /translation="MKTHVNEECPSCSSIGAIPASKTEENPRLSHWPIQIKLIGTVAP
                     FLKDADLLVAADCTAFAVHDFHKRFLKDKKVLIGCPKLDDAMSYVEKFTEIFSNISLK
                     SVSCLRMEVPCCGGMTAVLKEAINRSGKNISLTETIIGVKGDLLGERPVTG"
     gene            4671..4892
                     /locus_tag="VMU21_06020"
     CDS             4671..4892
                     /locus_tag="VMU21_06020"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HUO77118.1"
                     /translation="MKELNVIEIVPKERHPLIFRTFDELGRGEAFILTNDHDPKPLYY
                     QLLHEREGLFTWEYLAQGPEIWRVRITKN"
BASE COUNT         1259 a         1261 c         1292 g         1170 t
ORIGIN      
        1 ttactggcag ttttttcaac ccgcactatc tcgggaccgg agcaatgctg tggggtgcaa
       61 atgcctatga atatgcgggc aagtcataca gtgtcaatgc agcgcatcag agcgcaaact
      121 gtcccacctg ccatatggcc aattcgaccg ctgataacaa gaacggcggt cacacctggg
      181 cgccgaacgt ggtaacatgc aataccgctg actgccatgg cggcagcctc ggtcctgtgg
      241 cggcaaaatc tggcagcgca tctcctgatg tggataccta tcgggctagc tctgatacca
      301 ataactacac cggagaggcc ggaggagaaa ccctctctat tgcgcagtca attcaatccc
      361 tggagaagaa agtcatcgcc ctgcttcagg cgagggggat cttctatgac gacaccctgt
      421 acccctactt ctttgctgac gcaggacaca ctcaacagtt cactgcatgg actcctgcta
      481 ctttaaaggc agcctttaac ctttcctttg tggtaaaagg actgccttcg gcggcgacat
      541 cccaggtgct ggttcccaat gcatctgctg ctgtccataa ctacaagtat tgcatccagc
      601 tgttgctcga tgcgtatgcg gacctgagtg gagcatctct tgccggggcg gtaaggcctg
      661 cgggttcaag gcctgcaacg ggatacggtc cagggcagta aaattactta gagaggttac
      721 tcattacaca ttcaagcccc ggagcaatcc ggggcttttt tttaatccag atatttcagc
      781 ttcatttcga gaggctgttt cttggggccg ctgcttggat gggctgcata gcccaccggg
      841 accacggcca tgaattcacc cccaggctca ccaaaaaagg gcaggagatc gtccttgatc
      901 agataaagga tcccgagcca gaccgttccc agcccgaggg atgttgcggc aaggagcagg
      961 ttctgggcag ctgcggcaga gccctgtatc tccatggtac ggaaaaaatc atacgccatc
     1021 tctttttcga ccttgaacag ctctgtcccg tggctgataa ggtcaccggt gttcgcaatg
     1081 gcaatgacca ccggcgcgcc tgcgatactc tttgcagcca tgcgcagcag gactgaagcg
     1141 ggccgcggga agtcccctga cctctggttg atgagctgtg caagctcctg ttttttttcg
     1201 cccctcagca caataaacct ccatgactgc tggttatggg cagagggcgc ctcattggct
     1261 gaccggagca ggacatggat cagctcgtca gggaccggct gttttgtgaa ggtcctgaca
     1321 cttcggcgat gattaatcgt ttttaaggtc tcgttggaag gagaaacagt ttcagaggac
     1381 atagggttca ctccctgcat gaaaaagtct cataaaataa ttattacgta acatgttatc
     1441 acagatctga ccttggggca atgcatattg cggaattgac acgcctcggt caggcgaggg
     1501 ggtgagataa aaggaaggaa caggcagagg ctgaaagaat ctcacctctg cctttaccat
     1561 cctctgagct atgcttctga cttcgcgtat atggtgacga tcatgttgta gaagaagagg
     1621 aagatcgaac ccgcttccat taggccaaag gcaacggtca atcccttgta gattcccgct
     1681 tcaggatgaa agcttccgag ggtgtaaaag aagagcatgc cgacaagtcc tatatttgcg
     1741 agccagaact gaagctcacc catctttggg ctcttcaggg gcttacctgc gaacctcggc
     1801 aggatatggt agcccactcc gaagatcatc atcgagaccc atcccagcat gttgagatgg
     1861 gaatgaacga atttcagatg catcagggaa ggattaccca gcattaatat tcccagaaga
     1921 gatgccaccg caaggtagat gatactcatc aaaataaaat tcttcacaaa tctgtccata
     1981 aagactcctc ctcttatttt ctttaatgat actttacttt ctctgtggtt tcatccgcta
     2041 tgacggacgt catatgagcg gaatgtctat caaagggaaa acgtttcacc tgagcgatag
     2101 gtcatagtct tccctccttt atagattgtt actacgcctt ttccgagaac agtccattca
     2161 cctttcacac cgttatcgat cagtccggtc tgttcatcaa ccccaacgat cacatcgtac
     2221 gggatagacg gcaccagaga agatgcccaa ccttttccaa acgtattatg atgaggaaca
     2281 agacaggctt ggggaatcaa gtttagtcct tccctaatcc tttgtgtgtc cggatcgtag
     2341 tagtgctggc ataatatcat agcccctgca ctactgccac cgatcaccgc tcctgtgtcg
     2401 tatgcttcac gcatggcttg ccagcttaaa ctccccgcca gggtttgggc aagataatgc
     2461 gggaaaccgc ccagaagata aataagacgg gattggcgca acgcgctcgc tacagaaaat
     2521 tgattggctg aagcttgatc gataagaggc aacagagtaa cctgcttagc acctaaccgt
     2581 tcaaaccaac gaaccccgga ttggcctgca cgctggtggt tcctgtcggg aaaagcggcg
     2641 gtcggaacaa tgctcacctg aacatctgac ccgcctgcca gctcgatagc acgtttgtcc
     2701 ggctcagcca tttgcccacc gaactctgat ccgccttcta agaggatata gcctctcatt
     2761 tcctgacctt attatatcgc aatccaattc aggcctcacc taagcgcccc acttattgct
     2821 ataatgaacc tgaaagagat tcttgaaagt tttatcgttg gctcacaaca aggagggaga
     2881 gatggttgaa gattggctcg caaatgcata tgaaggcatg tcccggcgac agtttcttgc
     2941 ccggctgagt gctgccggag tcggcctcgc gggtttcgca atcgcctcaa gttcagtggg
     3001 aggtgaggtt attacgacct ctggtgatgg tcttgccgtg gccgacagta cggtgctctc
     3061 cggagatttc cctgttccga tctacggggc ccgaccggct gcatctggga aatatccaat
     3121 cctggttgta ataccggaga tttttgggat gcatgaacac atcaaagatg tcacccgccg
     3181 gtttgccaaa gagggtttct tcagtataac ctttgaacca tatgcgcgcg agggtggtgt
     3241 actgaatctt tccgacataa ccgcagtgcg ggctgtagct gactcggttc ctgataagag
     3301 agtcatggag gatttggatg cgatagtggc ctatgccaag cagcatccgt ctgcccgtgc
     3361 cgatcgaatt ggagtcacag gattttgccg tggtgggatg tatactctcc tctactctgc
     3421 ccataaccgt gaactgagag cctccgtggc gtggtacgga cagctcagtc cggaaaagaa
     3481 gccggggatc cgcacggtcg ggcctctgga tatagctgca cagatcgccc cgcctgttct
     3541 tggtctgtat ggtgaggatg atctgggtat cccggtagct gatgtcagga aaatggaagc
     3601 taatctcaag gcagctcaca agacgacaga gttcgtgctc tatcccggtg cgccgcacgc
     3661 tttttttgct gactataggc ccagttaccg gtccgatgct gccaaagacg cgtggagccg
     3721 ctgtctggca tggttcaaca agtatctcaa agggtagacg cttcccataa tgcactcaaa
     3781 agtggaatac gcatataata taatgttcgt gttcccaata gctgaactcg tgtattgaat
     3841 atgttggatt aaatagaaca caccccattt tctcaatggc tcatatctgg agaaggaaac
     3901 gcgggcaggt ctttaatctt ccgaacaatg attgttccgc atatatgcct tttaacgagt
     3961 tcctatctcg ttgtaacacg aggcgcaggt atggtcaatg ccgccggcat gcgtgaggct
     4021 tcccgcttct gtgagaactt cttctcagcc ttccctataa aacagcagca cgaaaccgaa
     4081 caaaatcata cacgcagtcc ggagggaatg gctgtatgat atacatcatg gcaggatggg
     4141 gatccgggga gtagactgaa tacaagagca atgaaaaatt atcagcagga ggaaagaaat
     4201 gaaaacacat gtcaatgaag aatgtccgtc atgttcatca attggagcga taccggcgag
     4261 caagaccgag gagaatccca ggctctcgca ctggccgata cagatcaagc ttattggaac
     4321 ggtagcaccc tttctgaagg acgcggatct gcttgtggct gctgactgca ccgcctttgc
     4381 agtgcatgac tttcacaaga ggtttctgaa ggacaaaaag gtccttatcg gctgtccgaa
     4441 gctggacgac gccatgtctt atgtggagaa gtttaccgag atcttcagca atatttcgct
     4501 aaagagtgtg agctgcctca gaatggaggt cccctgctgc ggcgggatga ccgcggtgct
     4561 gaaggaggcg atcaacaggt cagggaagaa tatctctctc accgagacca taatcggggt
     4621 gaagggcgac ctcctcggcg aacgcccggt cacaggataa gaggtgtgcg atgaaagaac
     4681 tgaatgtcat agagattgtt ccaaaggaaa ggcatcccct catcttcagg acatttgacg
     4741 aactgggcag gggcgaggcc tttatcctca ccaatgacca tgacccgaag ccgctctatt
     4801 accagctgct ccacgagagg gaagggctct tcacatggga gtaccttgca caggggcccg
     4861 agatatggag ggtcaggatc acgaaaaact gagaggtatg acacacgtca tatgcatgcg
     4921 cagatgcaca ggtgtatact aaaagaaaaa ggaggaagag tatggccaca acctcagaaa
     4981 ca
//
LOCUS       DATKOU010000131        10328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5171505, whole genome shotgun sequence.
ACCESSION   DATKOU010000131 DATKOU010000000
VERSION     DATKOU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5171505"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /gene="malQ"
                     /locus_tag="VMU21_06025"
     CDS             <1..1373
                     /gene="malQ"
                     /locus_tag="VMU21_06025"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HUO77119.1"
                     /translation="ILPLNPADQGSGNSPYSSISAFAGNTHLISPELLVESGLLKTDD
                     LGPIPKFPLERCDYAAAIPYKTALLHRAYERFKITGSGRDAYHAFCEQHKEWLEEFSH
                     YVVLKTRYEGKPWGEWSEGLRDRDPRNLGESLQGLHDEREREKFLQYLFFKQWHDLKV
                     YCNERAINLIGDIPIYVNYDSADVWTHADIFKLNGEKKPAFVAGVPPDYFSSTGQLWG
                     NPVYRWDVLKRTGFRWWKERIGHNLELFDSVRIDHFRGFVGFWEVPSGEKTAMNGRWV
                     EAPAAAFFRTLLKEFPSAPIIAEDLGVITPDVRKIMERFGFPGMRVLQFAFNADSATH
                     PYLPHNYRQDCIVYTGTHDNNTIRGWFEDEASHDEKQRLFRYLGREVSAEEVHNELIR
                     LAMMSVAQTAIIPMQDVLGLGRNARMNRPSTTNHNWEWRLLPDLLTSSHTEFLLEMTQ
                     IYGRAS"
     gene            complement(1404..5054)
                     /locus_tag="VMU21_06030"
     CDS             complement(1404..5054)
                     /locus_tag="VMU21_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HUO77120.1"
                     /translation="MIKGKKFFLWIAGGTGTLLLILLALYFLIPRLVDLNSIREKIKT
                     GISQKIAGTVDFQKMEVSLFPRPRILINQARFSFPGTAEGTIDSLNIYPHILPLLSGK
                     VRIGRIQIEAPHVSLTITEREKDRKKTPLSAGDIEEKVSAFLNVLASNVPGTSIGIEK
                     GSVTISDITQFLFSFQDIEGQIVLPPGEASVTLTCSSNVSKSMSFGMRLDPKNLKGMG
                     TLKLTGMKPQPVVQHFFPDFVRRLGDSEANLMLSFKILGFKDIQAEVQGSFPYLTLLS
                     KNDKVPLKGIGIHATLLLTGDKTEITLSELKIEHPRLTLSGALVMDKSIQNVSMEISG
                     SQVDVPSLRNVALSLAGEVPVVQNVFSYVRGGKVPFITVRSQGASLEDLGKTESIFIK
                     GVIQKGEIFVPGPKLDFKEVNADCIISRGILDGTNVEGNLGNSQVRDGKLRVGLKGSD
                     APLHIDAAVKADLAEARNILGRLIKDEPFLKEIKLINTLHGEAKGRLILGESTAFIKP
                     RVDLSEVSFTAEYQRVPFPLVIKGAQFSYDETRIGVKKLSGTLGKSSFAELAGELRLG
                     DAPSIEIPSGKLRIVQDEIYPWLKSLEGLKGSLEDLRSARGVINLDTINLKGPLSKPK
                     QWHFRLAGNVEDTVVDLAFLTAPVSLKKGAFDANEEKISLTDARTEILDASVALSGIQ
                     QGYLGGKRKTDLTFQGKMGPQSFEWAAKLASLPSQFSPKDSITFEHAHIAVDEGTALF
                     QGSLRVQKGPKITADVLKHPKGLAVNKLIIKDASSDATLSLDLQAKAFKLAFAGKLTS
                     ETLGKLISEKEFPQGRIAGDFRAEIQREKPFQFTATGKIRGENIVMPSKWGLPIKIEN
                     LSLAGDGSSLSIEPSSLRVDDTPFSLKGNLNFTKGDLAIDMDLSADRIEWQKITQALK
                     GTGKKDSEETNDSKESNQSKETWGIPVKGTVRLKTNYFTYDKYTWTPLHADISLQRDD
                     IDIAVTKATLCGISSQGTMNVTPKALSLDFQLTADNQELEPALVCLFDKKRDATGKFG
                     LKARIKGQGNKETLRDSLEGDGEFVATDGRIFRLPALLNIFSFLEVNQILRGFPEIRK
                     EGFAYHSIKVKEGLRQGTLEIKEAIVDGSTMKIATQGTVDLRNDTLDLTVLISPMKKT
                     DFIIKKVPIIGNILGGSLVSIPVKVSGDMEDPKVSSHPVSAVGSGLVGIMERTLKAPM
                     KLFDPFLPDEKK"
     gene            complement(5240..5602)
                     /locus_tag="VMU21_06035"
     CDS             complement(5240..5602)
                     /locus_tag="VMU21_06035"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HUO77121.1"
                     /translation="MIRLVYCVSRSKDISVEEFRRYWNDEQFSVLMNAFKKLYKAIKT
                     TKNLVLNVPMNTTMLERQGTREPYDGIIELWWESAKYLIALNETSEAEGFRQKMRAFE
                     KQFIDKSRSTIFFSEYFE"
     gene            complement(5640..6434)
                     /locus_tag="VMU21_06040"
     CDS             complement(5640..6434)
                     /locus_tag="VMU21_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUO77122.1"
                     /translation="MYKSRVGITLLILSLFVLGGTTTAAPPLVTEAKNSPLQVGVTPD
                     NPPMIFKINDRLTGAEADFAKLLGKELDRQVQFVELPWNQQINALLDGRIDIIMSSMT
                     ITEARKVRITFTDPYLKSGLGAAIRASDASKYRSVKNIKEDFPAVGVMEGTTSEAYVR
                     KNMPNSPRILALRKLSDAAIELKNRRIDVFIYDAPAIVWLVSENEADLRGVWELFNEE
                     HLGWGVRRDDKELLLRVNTVLNKWKNDGTLKEVLSRWLPYWKNFEF"
     gene            6675..8582
                     /locus_tag="VMU21_06045"
     CDS             6675..8582
                     /locus_tag="VMU21_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HUO77123.1"
                     /translation="MRNNKDIDDDRDNVLGSSLVDESERFLKEMVAGSMEGYEIRRPQ
                     MEMMNACSRNIVKGGTLLAEAGTGTGKTFAYLIPIILSGKKAIVSTKTINLQEQLVSK
                     DLRFLSGLKEFPYAIAKGRGNYLCLRRFNAFRAEGERDAIEYRMLQMWVANTDTGDVE
                     DLDIKRSFIWDKVCSDSDACRGVKCGHYNRCFYFRARKRWEMAQVVVANHALIGINSM
                     LTDNSRMLPQAEVLVIDEAHALDSVLSEVIGITLSNRGFGNIMGRLLKLDERGTYKGL
                     LSGSSQLFPSVTSLATEMELLWNQVRNEFRDKDIIRDIFKFGDMAEGLAESLRSLIME
                     IRATTTGLFKEDEEIELGASLLKLKAFSDGLEGFVSGMDGYVRWIEREEGKTALKMVP
                     VYPGDFVAHHIMPEFGSVVLTSATLSVSGDFHFISQVLGLTDPATITLPSPFDLKRQM
                     VIEIRRGIDLKKDEGTVGLSEVVLEEASKKDGGILVLFTSREVMKRTWDLCSEQLNNM
                     DLNPMIQGELSNRTMLQAMRESENSIIFGLDSFWEGVDVRGDSLKCLIITKLPFEVPT
                     EPIALARTEIIEKEGGNSFYEYSLPKAVLKFKQGFGRLVRSKTDTGRIVICDERIETK
                     AYGRMFKNVFM"
     gene            8765..9259
                     /locus_tag="VMU21_06050"
     CDS             8765..9259
                     /locus_tag="VMU21_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HUO77124.1"
                     /translation="MKGFSRFSMLALVVCLLALFTGGMVTDSHAYRGKGGDPMMKQLL
                     KGIGLTDQQKAQIKEIIQTHRNELLTGKVAVLQARQNLLAVMTSSTYDKSAVNVAYNT
                     LAVAQQNMADVRAQIFNAVITTVLTPDQQAAVQGRIAAKNQRIQRVIAGLQAKLKTPP
                     LGTQ"
     gene            9349..9825
                     /locus_tag="VMU21_06055"
     CDS             9349..9825
                     /locus_tag="VMU21_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77125.1"
                     /translation="MKKVICLVLATLLLLMTSTVADHACAQGGSHGGGGGSHGGGSHG
                     WGGGSHGWGGSHGGHWSGGVWIGPGWGWGWGWGGWGPWWWGAPYYPYYPYYPYYASPP
                     VMEQQPHVYVEPTPQQEEQSYWYYCRKPQGYYPYVQKCPGGWLKVIPSSVPPDVKE"
     gene            9830..>10328
                     /locus_tag="VMU21_06060"
     CDS             9830..>10328
                     /locus_tag="VMU21_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HUO77126.1"
                     /translation="MTKFGKSIFLLLILLVVGGCATLPTGPSVMVLPPPNKPFEQFQA
                     EDAICRQWAAHQIGMSPQQAVNQDTATGAVAGTAIGAGLGAAIGSASGNAGVGAAIGA
                     GSGLVVGTAAGANAGQASGYEAQRRYDIAYQQCMYSKGNQIPGVVTRTRTIRTVPPPP
                     PPPGYG"
BASE COUNT         2505 a         2494 c         2704 g         2625 t
ORIGIN      
        1 agatattgcc gctcaatccc gcagaccaag ggagtggaaa ctcaccctat agcagcatct
       61 ctgcattcgc aggaaatacc caccttataa gccccgaact gctggtggag tccggactcc
      121 ttaagacaga tgaccttgga ccgataccga agtttcctct ggagcggtgt gactatgcag
      181 cggctatacc gtataagaca gcgctcctgc atcgcgccta tgagcgtttt aaaatcacag
      241 ggagcgggag agatgcgtac catgcattct gcgaacaaca taaggagtgg ctcgaggagt
      301 tttctcacta tgttgttctg aaaactcgtt atgagggaaa accctggggt gaatggagtg
      361 aggggctgag ggacagggat cccaggaatc tgggagagtc attgcagggt cttcatgatg
      421 agagagagcg ggagaagttc ctccagtacc tctttttcaa gcagtggcat gatctcaagg
      481 tctactgtaa tgaacgggcc attaacctca tcggggacat tcctatctat gtgaactatg
      541 acagtgctga tgtgtggaca catgcagata tcttcaagtt aaacggagaa aagaagcctg
      601 cttttgttgc cggtgttcct cctgactatt tcagcagcac cggacagctc tgggggaatc
      661 cggtataccg gtgggacgtt cttaagagga caggtttcag gtggtggaaa gagcgtattg
      721 gccataattt agagctcttt gacagcgtgc gaattgacca cttcagaggt tttgtagggt
      781 tctgggaagt gccttcagga gagaaaaccg ccatgaatgg ccggtgggtc gaggcccctg
      841 cagcagcctt cttcaggacc cttcttaagg aatttccctc tgcacccatc attgcagaag
      901 acctgggtgt tattactcct gatgtgagga agatcatgga gcgtttcggg ttcccgggaa
      961 tgcgtgtcct ccagttcgcc tttaatgcag attcggcaac ccatccctat ttgccgcaca
     1021 actacagaca agactgcatc gtctacacag gaacacatga caacaacacg ataaggggat
     1081 ggttcgaaga cgaggccagt catgatgaga agcagcggtt gttccggtat ctcggaagag
     1141 aggtctctgc tgaagaggtt cataatgagc tcatccgcct tgccatgatg tcagtggcac
     1201 agaccgccat tataccaatg caggacgttc tcgggcttgg acgaaatgcc cggatgaatc
     1261 gtccgtctac aacaaatcac aattgggagt ggaggctgct tccggacctg ctgacctctt
     1321 cccatacaga gtttcttctt gaaatgacgc agatttatgg aagggcatcg tgaagagagc
     1381 gctggccctg tttgtggcga tgtctacttc ttttcgtccg gcaggaaggg gtcaaagagc
     1441 ttcatagggg ccttgagggt cctttccatg attcctacta atcctgaccc tacagctgaa
     1501 acaggatgag agctcacctt tgggtcctcc atatctccgg agaccttaac ggggatggag
     1561 acgagcgaac ctccgagaat gttgcctatg atggggactt tcttgatgat aaaatcagtc
     1621 tttttcatgg gagagatgag gacggtaagg tccagtgtgt cattcctcag atcgactgta
     1681 ccctgggtag cgatcttcat agtagaacca tccactatcg cttcttttat ttccagagtg
     1741 ccctgcctca gcccttcttt gacctttatg gagtgatatg caaaaccctc tttccttatt
     1801 tcagggaacc ctctcaggat ttgattgact tccagaaagg agaaaatgtt caagagagca
     1861 ggcagacgga aaatccgccc gtctgtggca acaaattccc catctccctc gagagaatca
     1921 cgcagcgtct ctttattccc ttgtcccttg atccttgctt tcaggccaaa ttttcccgtt
     1981 gcgtcccgtt ttttgtcaaa gagacagaca agggccggtt ccagttcctg attatctgca
     2041 gtgagctgaa aatcgaggga gagcgctttc ggcgtcacat tcatggtccc ctgcgaggaa
     2101 atgccgcaga gagttgcctt ggtcaccgca atatcaatgt catcacgttg aagggatata
     2161 tccgcatgga gaggtgtcca ggtgtatttg tcgtaggtga aataattcgt tttcagcctg
     2221 acggtcccct taacgggtat gccccatgtt tctttgctct gattgctctc tttgctgtca
     2281 ttggtttctt cgctgtcttt tttcccggta cctttgagag cctgtgtgat cttttgccat
     2341 tcgatccggt ctgcggagag gtccatatcg atcgctaagt cccccttggt gaaattcaga
     2401 ttccccttaa gagaaaaagg tgtgtcatcc accctgaggg aagaaggttc tatggaaaga
     2461 ctgctgccgt cgccagccaa agagaggttt tctattttta tcgggagtcc ccacttggaa
     2521 ggcatgacga tattctctcc cctgatcttc ccagtggcgg tgaactgaaa cggcttctcc
     2581 ctctggattt ctgcccgaaa atctcccgcg atccttccct gggggaattc tttttctgag
     2641 atgagcttac ccaatgtttc tgaggtcagt tttccggcaa aggcaagttt gaatgccttt
     2701 gcctggagat cgagggagag ggttgcgtcg gatgaggcat ccttaatgat aagcttattc
     2761 acggcaagcc ccttcggatg cttgagcaca tcagcggtga tcttcggccc cttctgaacc
     2821 cgcaagctcc cctggaaaag cgcagtgccc tcgtccactg cgatatgtgc atgttcgaac
     2881 gtaatggaat cctttggaga gaattgagag ggcaggcttg cgagctttgc agcccattca
     2941 aaggactggg gccccatctt tccctgaaag gtgaggtccg ttttgcgctt gccccccaga
     3001 taaccctgct gaatgcctga cagggctacc gaggcatcga ggatctctgt ccgtgcgtct
     3061 gtgagggata tcttttcttc atttgcatcg aaagctcctt ttttcaaaga gacaggagca
     3121 gtgaggaacg ccaagtctac taccgtatcc tccacattgc ctgccagcct gaaatgccat
     3181 tgcttgggtt tcgacaaggg acctttcagg ttgatggtgt caagattgat gacgccccgc
     3241 gctgagcgca ggtcttccaa agaacctttt aatccttcaa gggacttcaa ccacggatat
     3301 atctcatcct gtacgatcct tagtttccct gatgggattt caatggaagg ggcatcccct
     3361 agacgtaatt cgccggcaag ctctgcgaag gaggacttgc ccagcgtgcc gctcagtttt
     3421 tttacgccga ttcgcgtctc atcataagaa aactgagctc ctttaatcac aaggggaaag
     3481 gggactctct gatattcagc agtgaaggag acttcagata aatcaacgcg cggcttgatg
     3541 aaggcggtgc tttctccaag gatgagcctt ccttttgctt caccgtgtag ggtattgatg
     3601 agtttgattt ctttcaagaa cggttcatcc tttatcagac gtcccaggat gttcctcgcc
     3661 tctgcaaggt ctgccttgac cgctgcatcg atgtggaggg gggcatcact ccctttcagt
     3721 cccaccctca gttttccgtc tctcacctgt gaattcccaa ggtttccctc gacatttgtt
     3781 ccgtccagga ttcccctgga aataatgcaa tctgcattga cctctttgaa atccagtttt
     3841 ggaccaggga caaaaatctc tcccttctgt ataactcctt tgatgaatat gctctctgtt
     3901 tttccgaggt cttcaagtga tgcgccctga ctgcgcacgg tgatgaaggg aacctttcct
     3961 cccctcacat aactgaaaac attctgcacc acgggcacct ctcctgccag cgagagagcc
     4021 acattcctca aggatgggac atccacctgg ctgccggata tctccatgct gacattctgt
     4081 atgcttttat ccatgacgag cgcaccgctc agggtgagcc gagggtgctc aatcttcagt
     4141 tcgcttaatg ttatctccgt cttgtcgccg gtcaggagaa gggttgcgtg aatcccaatt
     4201 cctttcaggg gaaccttatc gttcttactc aggagggtaa ggtaagggaa tgagccttgc
     4261 acttctgcct gaatgtcttt gaacccgaga atcttaaatg agagcatgag atttgcctca
     4321 gaatctccga gacgcctgac aaagtcaggg aaaaagtgct gaacaacggg ctgtggcttc
     4381 atacctgtaa gtttgagggt acccatgcct ttgagattct ttggatccag gcgcatacca
     4441 aaggacatgc tttttgagac gttggagctg caggtgaggg tgacactggc ctctccggga
     4501 gggagaacga tctggccctc aatgtcctgg aatgagaaaa ggaactgggt tatatcagag
     4561 atggttacac ttcccttttc tataccaatg ctggtgcccg gcacatttga tgccagtacg
     4621 tttaaaaacg cagaaacttt ttcttcgatg tcccctgcgg agagaggcgt tttcttcctg
     4681 tctttctctc tttctgtaat agtgagagat acgtgggggg cttcaatctg aatcctcccg
     4741 attctcacct ttccggagag aagcggaagg atgtggggat aaatgtttag tgaatcgatg
     4801 gttccttccg ctgttcccgg aaaagagaat ctcgcctggt tgatcagtat cctggggcgg
     4861 ggaaacagcg agacctccat cttctggaaa tctacggtgc cggcgatttt ctgggaaatg
     4921 ccagtcttta tcttttccct tatggaattg agatcaacga gccttggtat aaggaagtag
     4981 agcgccaaca ggatgaggag aagagttcct gtcccccctg cgatccacaa aaaaaacttc
     5041 ttccccttaa tcataggctc tttcattatg agactttgaa tacccctcta tcgatatatc
     5101 ctgcccctgc ctccctcttt tcacacgggt aaacagaaaa ggcatgacgg ttaccatatg
     5161 aaaggaaagc agcatgggaa gcgattgtgt gctactttcc cttcaaaggg aaaactgtac
     5221 gaatacgtaa agtgaccaat cactcaaaat attcggaaaa gaagatcgtg gagcgggact
     5281 tgtctataaa ttgtttctca aacgccctca ttttctgtct aaacccttcg gcctcggatg
     5341 tttcattaag ggcaatcaga tacttggcgc tctcccacca cagttcaatg atgccgtcgt
     5401 aaggctccct tgttccctgc ctctccagca ttgtggtatt cataggcacg ttcagcacga
     5461 gattcttggt ggtcttaatg gccttgtata attttttgaa agcgttcatt aggaccgaga
     5521 attgctcatc attccagtaa cgccggaatt cctcgactga gatatctttg ctccggctga
     5581 cgcaatacac caatcgaatc atgctgcctc cttttctgcg tttcgcatga ttgtcacatt
     5641 cagaactcga aattcttcca gtagggaagc catctggaga gtacctcttt caacgtacca
     5701 tcattcttcc atttattgag tactgtgttc acccgtagga ggagttcttt gtcgtccctc
     5761 ctcactcccc atccgagatg ttcctcgttg aacagctccc agacgcctct gagatccgct
     5821 tcgttttccg agacaagcca tactatggca ggggcatcgt agataaagac atcgatcctt
     5881 ctgttcttta gttcaatggc agcgtcgctc aactttctga gcgcgagaat ccttggcgag
     5941 ttcggcatat tcttgcgcac gtatgcctcg ctggtcgtcc cttccatcac ccccacagcg
     6001 gggaaatcct cctttatatt tttcactgac ctgtacttcg atgcatccga tgcgcggatg
     6061 gcggcgccta ggccgctctt aaggtaggga tcggtgaagg taatcctaac cttccgagcc
     6121 tcggtgatgg tcatggagga catgatgatg tcgattctcc cgtccagcag ggcgttaatc
     6181 tgctggttcc atggaagctc cacaaactgg acctgcctat caagctcttt tccgagaagc
     6241 ttggcgaagt cggcctctgc tccggtgagc ctatcattaa ttttgaagat catgggcggg
     6301 ttatctggag ttaccccgac ctgtaagggg gaattcttgg cttctgtcac caggggtggg
     6361 gctgctgtcg tggtaccccc cagaacaaat agtgagagga taagcagcgt aatgcccacg
     6421 cgtgatttat acatgaatgg tagtctcctt tctctacaaa ttttcattaa tgattatacc
     6481 ttttaaagag aacagacaca acatctttca tcccgctgcg cagaaaacta ttcagtcatc
     6541 ggctgatggg ggaatggaat aattcggtgg attaagaccc tgagagatgg agaaattatc
     6601 ctgcctgccc acaaggaaag tgctgaggag aggtgacaga ttgtccccca agatactatt
     6661 attaatcaga acgtatgcgg aacaataaag atattgacga tgaccgggat aatgtcctgg
     6721 gttcatcact ggtggatgag tctgagaggt ttctgaaaga gatggtcgcc ggatccatgg
     6781 aaggctatga gataaggcgc ccgcaaatgg agatgatgaa tgcctgttcc cgaaatatcg
     6841 tgaaaggggg gaccctcctg gcagaggcag ggaccggcac aggcaagacc tttgcatatc
     6901 ttattcccat catcctttca ggcaagaagg ccattgtatc cacaaagacc ataaacctcc
     6961 aggaacagct tgtctccaaa gacctgcggt ttctctcagg gttgaaggag ttcccttatg
     7021 ccatagccaa gggaagaggg aactacctct gcctcaggcg tttcaatgcg ttcagggccg
     7081 aaggagagag agatgccatt gaatacagga tgctccagat gtgggtcgcg aacacagaca
     7141 caggagatgt ggaagacctt gacataaaaa ggtcattcat atgggacaag gtatgctcgg
     7201 attcagatgc ctgcagggga gtcaagtgcg gccattacaa ccggtgtttc tatttcaggg
     7261 caagaaagag atgggagatg gcccaggttg tggtggcgaa ccatgccctc ataggcatca
     7321 acagtatgct taccgacaat tcaagaatgc tgccacaggc ggaagtcctt gtcatagatg
     7381 aggcgcatgc actggacagt gttctttctg aggtaatagg cattaccctg tcgaacaggg
     7441 gattcggaaa cattatgggg agacttctca aactcgatga gagggggacc tacaagggtc
     7501 tcctctcagg gtcttcacag cttttcccct ctgttacgtc gctggcaacc gagatggaac
     7561 tcttgtggaa ccaggtgagg aacgagttca gggacaagga catcatacga gacattttca
     7621 aatttggcga tatggctgaa ggactcgcgg aatcgctccg gtcactgatt atggaaatac
     7681 gggccaccac cacaggactg tttaaggaag acgaagagat cgaactcggg gcatccctcc
     7741 tcaaactcaa ggcattctct gacggactgg aaggatttgt cagcggaatg gacggctacg
     7801 tgcggtggat tgaaagggaa gaagggaaga cagcgctgaa aatggtgcct gtctatccag
     7861 gggactttgt tgcccatcac atcatgcctg agttcgggtc ggtcgttctc acatcggcca
     7921 ctctttctgt atcaggggat ttccatttta tatcgcaggt tctcgggctg acagatccgg
     7981 caacaataac cctgccatcc ccttttgacc tcaaaaggca gatggtcata gagataagaa
     8041 gaggaattga cctgaaaaaa gatgagggga ctgtggggct ttctgaagta gtgcttgagg
     8101 aggcttcaaa aaaagacggg gggattcttg tactcttcac ctccagggaa gtgatgaaaa
     8161 ggacgtggga cctctgttct gagcagttaa acaatatgga tctcaacccc atgatccagg
     8221 gtgagctatc caacaggaca atgctccagg caatgcgtga aagcgaaaac agcattatat
     8281 tcggccttga ttccttttgg gaaggggtgg atgtcagggg ggattccctc aaatgtctta
     8341 tcattacgaa actgcccttt gaagtcccca ccgaacccat agcattggca agaacagaga
     8401 ttatcgaaaa agaggggggc aactcatttt atgagtactc ccttcccaaa gcggtgctga
     8461 aattcaagca gggattcggg agactggtca ggtcgaaaac cgataccggc aggatcgtga
     8521 tctgtgatga gaggatcgag acaaaggctt atgggcgtat gtttaagaat gttttcatgt
     8581 aatcaatgag ggaacttctt gctggtaata cttaatctca tccggtaatt aatttgacaa
     8641 agagtaagga tgtatctgcc tgccttatca tgatccgaat acctgttaaa ccttttttag
     8701 gggtcggtgt ctaattggat atggttgctg taaaagaaca atctttttca aaggaggaaa
     8761 taaaatgaaa ggttttagcc ggttttcgat gcttgctctg gtggtttgtc tcttggcgct
     8821 atttaccgga ggcatggtta ccgattcaca cgcgtatcgt gggaaaggtg gtgatcccat
     8881 gatgaagcag ttattgaagg ggatcggtct cactgaccaa cagaaagcgc aaattaagga
     8941 aatcatccag actcaccgca atgaactgct tacagggaaa gttgctgttc ttcaggcccg
     9001 gcagaatctg ctggcggtga tgaccagtag cacctacgac aagagcgccg taaacgtagc
     9061 ctataatacc ctggcagttg cccagcagaa tatggctgac gtacgggcac agattttcaa
     9121 tgcagtaata acgaccgtgc tgactcctga ccagcaagcc gcagtgcaag ggaggatcgc
     9181 agcaaaaaac cagcgaatac agagggttat cgccggactc caggcaaaac taaaaactcc
     9241 accccttggt acacagtaat ctgcttatgc tgcaatgggc aacagtggat tgactgcatt
     9301 ggatggtgta aaataattat atacaagacg atctttatgg agatgaaaat gaagaaggta
     9361 atttgtctcg tcctagcaac cctgctactg ctcatgacaa gcaccgtagc cgatcatgca
     9421 tgtgctcaag gaggctctca cggaggcgga ggaggctccc atggcggcgg ctctcatggc
     9481 tggggaggag gctcccatgg ttggggtggt tctcatggag gtcactggtc tggaggtgtc
     9541 tggataggcc ctggctgggg atggggttgg ggatggggtg gatggggtcc gtggtggtgg
     9601 ggtgcaccgt actacccata ctacccatac tatccatatt atgcatcacc gcctgtgatg
     9661 gagcagcagc ctcatgtata tgttgagccg acccctcagc aagaggagca gagttactgg
     9721 tattactgca ggaaacccca aggctattat ccctatgtgc agaaatgtcc tggcggctgg
     9781 ctgaaggtca ttccttcttc tgttccgcct gacgtgaagg agtgaagaga tgacgaaatt
     9841 cggaaagagc atctttttat tattgattct gctggtggtg gggggatgtg cgaccctccc
     9901 cacaggtccg agtgtgatgg tgttgccacc gccgaacaag ccctttgagc agttccaggc
     9961 cgaggacgcc atctgcaggc agtgggcagc acaccagatt ggaatgagcc ctcagcaggc
    10021 ggtcaatcag gatactgcca caggggctgt cgctggcacg gccatcgggg caggcctcgg
    10081 cgctgccatt ggctcagcct caggcaatgc gggtgttggc gctgccattg gcgcaggaag
    10141 cggacttgtt gtgggaacgg ccgcaggcgc caatgccggt caggcgtctg gatatgaggc
    10201 gcagcgtcga tacgacatcg cataccagca gtgtatgtac tccaagggga accagatccc
    10261 cggggtggtg acacggaccc gtacaatccg gacagtccct ccaccacccc ctccaccggg
    10321 ctacggct
//
LOCUS       DATKOU010000132         5221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_166649, whole genome shotgun sequence.
ACCESSION   DATKOU010000132 DATKOU010000000
VERSION     DATKOU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166649"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VMU21_06065"
     CDS             <1..276
                     /locus_tag="VMU21_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77127.1"
                     /translation="QGWEDAELYLRAIVESEFFLGKNKSLPSLKHVKTVEDAVNFALG
                     FEKETLLYFYGIRDAVKEREVVEEIINEERSHIMWLHRFKDSFRKKK"
     gene            485..1306
                     /gene="rsmI"
                     /locus_tag="VMU21_06070"
     CDS             485..1306
                     /gene="rsmI"
                     /locus_tag="VMU21_06070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HUO77128.1"
                     /translation="MKGILYIVSTPIGNLEDITLRALRILKEVDLVAAEDTRHSSKLL
                     SYYGISKPLISYWGEKEKVKSEEVLARLQAGQSVALVSDAGTPGISDPGNVLIEKAIK
                     DGISVVSVPGPSALIAAISLSGLLIKEFTFVGFLPPKKGERLRMLRDLGMEKRALIFY
                     EAPHRILESIEDMAQVFGERKAAVVKEITKVHEEVLRGSLTDIIRLLQGTVIAGEYVV
                     VVEGRQKGEASFEDALDEVLALMKKGRGRKEAVKIISEQYGLSKNDLYEKSLLET"
     gene            1281..1859
                     /locus_tag="VMU21_06075"
     CDS             1281..1859
                     /locus_tag="VMU21_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUO77129.1"
                     /translation="MKRVCLRPDLKACRGGPMLWGTLCFFITCIAQYFLYNGGVLATE
                     RKERDQAVEKRVVELVNHARAQGIKCGSTYYRAASAVAWNDTLGKASLMHSLDMAEKG
                     LLYHTGSDRRDPGDRITRLGYVWSAYGENVAEGYHSPEEVVKGWLKSKGHCENIMNPS
                     FKEAGSAHVRGQGGIYWTLLLAAPGKMMSLAP"
     gene            1888..2505
                     /locus_tag="VMU21_06080"
     CDS             1888..2505
                     /locus_tag="VMU21_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HUO77130.1"
                     /translation="MGLLLIGFSSFIIALSGALVPGPLFSITVAESAKRGASAGPLII
                     IGHGLLEISLITLIILGITPFLSSLSAKTIICAVGGLVLIYMGYRLIKDAGAARLFLT
                     GGEEKKGLHPVFTGIVGSVSNPYWSIWWVTIGLGYLVSSLKFGFLGVIVFFIGHIMAD
                     IAWYSLISFAVARGKEMMGDRGYRSLLYACGVFLVFFGIWFMKGI"
     gene            2568..3704
                     /locus_tag="VMU21_06085"
     CDS             2568..3704
                     /locus_tag="VMU21_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HUO77131.1"
                     /translation="MSKVYFSSARVQKWKYDESMPGKLERLLREIGLSRYFSPKEWVA
                     VKTHFGSEGAHRVVRPVFLRKVVEGLKDVGARPCVVDTVRIKGLDYLEVANQNGINHL
                     SVGAPVLLADGIYGNDNIMVRAGEILGEIAVASLIHDIPAMVVCSHVKGHINAGYAGA
                     IKNLAMGGVSASHRHCGWKCGRGAMHTIGEGALVWNSEQCELCYQCAEVCPLDAIVFE
                     EGDELVWNGDTCWRCGRCTRVCQNEALHLPGDDERFLRSLAEAAKAVLGTFDPLKIIY
                     VNFLSEIQPECDCMPIADVPVIQDKGILVSDDLVAVEQASIDMLRNSEPLPESAASKE
                     KLGSDRDILLGLHKKNYLIQVDEAERLGLGSRTYELIEIPQKEK"
     gene            complement(3905..5110)
                     /gene="argJ"
                     /locus_tag="VMU21_06090"
     CDS             complement(3905..5110)
                     /gene="argJ"
                     /locus_tag="VMU21_06090"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HUO77132.1"
                     /translation="MNNKKSPTNIITPEGFFFSTVEAAIKKPGKKDLLLIYSPREAIM
                     SGMFTTNQVKAAPVKLDMQRIRAGKGQAIIVNSGNANACTGKQGMRDALAMTRLIAKG
                     LNLEQDKVYVCSTGVIGTPMPMERVRAKIPELVTGLGKSTPDDAAAAIMTTDTFPKVV
                     TRRIRIGSKTGTLVGICKGAGMICPHMATMLCFIMTDLKIGKGALDKALKDTVETSFN
                     SITIDGDMSTNDTVLIMANGMAENEPIKRNTAAFMRFSSALSDITYELGRLIVKDGEG
                     ATKLVEVVVKNARKQWDAKKAALSIANSPLVKTALYGNDANWGRIMAAMGSSGIAIRE
                     TRTDIYFGKVRVVKNGISQGKDMEAARIIRGKEVTITVDLRLGSSSSRVLTCDLTEDY
                     IRINAEYRT"
BASE COUNT         1267 a         1131 c         1437 g         1386 t
ORIGIN      
        1 cagggctggg aagatgcgga actctatctc agggcaattg tggagtctga gtttttcctc
       61 ggcaagaaca agtcgcttcc ttccctgaaa cacgtcaaga cggtggagga tgcggtgaac
      121 tttgcccttg gatttgagaa agagacactt ctctattttt acgggatacg ggatgcagtg
      181 aaagaaaggg aagtggtgga ggagattatc aacgaagaga ggagccatat tatgtggctc
      241 caccggttta aggacagttt taggaaaaag aaatagcgat acggtgtgtc agggtccaaa
      301 accattaggt gcgattttgc gataagctgt aatggattgt ccataaagaa tggagactct
      361 gtgaaccaat attcaccctt gaggattgcc ggtcatcacg agtcttggat agagtgagcc
      421 aacacataaa gatgtcgcta aagttttttc tcccggccat aaccgattca ccaataggac
      481 cactatgaaa gggattcttt atattgtttc aacccccatt ggcaatctgg aggatatcac
      541 cctaagggca ttgaggattc ttaaggaagt tgatctcgtc gctgctgagg atacgaggca
      601 ctcctcaaaa cttctcagct attacggcat ctcaaaacct ttgataagct actgggggga
      661 aaaggaaaag gtaaagtccg aagaggtact tgcaagattg caggcaggac agtcggtcgc
      721 cctggtctct gatgccggga cccccgggat atcagaccca ggaaacgtgc tcatagagaa
      781 ggcgatcaaa gacggtatct ctgtggtgtc tgttcccggt ccttctgccc tgatcgctgc
      841 catttccctc tcagggcttc tgataaagga atttacgttt gtcggttttt tgcctccaaa
      901 gaaaggggaa cgtcttagaa tgctgaggga ccttggtatg gagaagaggg cacttatctt
      961 ttacgaggca ccccatagga tcttggaatc cattgaggac atggcacagg tgtttgggga
     1021 gagaaaggct gcggtggtaa aggagattac taaagtccat gaagaggtgc ttagggggag
     1081 tcttacggat attattcgtt tgctccaggg tacggtaatt gcgggtgaat acgtggtcgt
     1141 ggttgaagga aggcaaaagg gagaggcctc atttgaggat gcccttgatg aggtactggc
     1201 ccttatgaaa aagggcaggg ggagaaaaga ggcggtaaaa ataatctctg aacaatacgg
     1261 gttaagcaag aatgacctgt atgaaaagag tttgcttgag acctgatctc aaggcatgca
     1321 ggggcggtcc aatgctctgg ggcacgcttt gtttcttcat tacctgcatt gcgcagtact
     1381 ttctatacaa tggcggggtt ctggcaacgg aacgcaaaga acgggatcag gctgttgaga
     1441 aacgggttgt ggaattagtg aatcatgcaa gggcacaggg cataaagtgt gggagcacat
     1501 actacagggc tgcttctgct gttgcgtgga atgataccct tggaaaggcg tcattaatgc
     1561 attccctgga tatggcggag aagggtctct tataccatac aggttctgac aggagagatc
     1621 ccggggatag aatcaccagg cttggatatg tatggtctgc atatggtgag aacgttgcag
     1681 agggttatca ttctcctgaa gaggtagtaa agggctggtt aaagagcaag ggtcattgcg
     1741 agaacatcat gaacccttcc tttaaagagg cgggctctgc ccatgtccgg ggccagggag
     1801 ggatctactg gactttactg ctggctgcgc cggggaagat gatgtccctc gcgccttaag
     1861 aatttgaaga aagagggcgt tacggagatg ggtctcttgt tgataggttt ttcatcattc
     1921 attatcgccc tttccggggc gctggtcccc ggtccgcttt ttagcattac cgtggcggaa
     1981 tccgcaaaaa ggggtgcatc agcagggcct ttgataatca tcgggcacgg actattggaa
     2041 atcagcctta tcactctcat cattttgggc ataacgcctt tcctctcttc cctgtctgca
     2101 aagacaataa tctgtgccgt gggcggtctg gtgcttatct acatgggata ccgattgata
     2161 aaggatgcag gggctgccag gctgttcctg accggggggg aggaaaagaa gggtttacat
     2221 cctgtattca caggaatcgt ggggagcgtt tcaaaccctt actggagtat atggtgggtc
     2281 acgattgggc tgggttatct cgtaagctcc ctgaagtttg gcttccttgg ggtaatcgtt
     2341 ttttttatcg gccatatcat ggctgacatc gcatggtaca gccttatctc ctttgctgtg
     2401 gcaagaggga aagaaatgat gggggacagg ggatataggt ctctgctgta tgcctgcggc
     2461 gtgtttcttg tcttcttcgg catatggttt atgaaaggga tttgaaaggg attgagactt
     2521 tcgcattcag gctcgattta gaggtctttg gataaaggga ggactctatg tcaaaagtgt
     2581 atttttcctc agcaagggta cagaaatgga aatacgatga aagtatgccg ggtaagcttg
     2641 agaggctcct cagagagata ggtctttccc gttatttcag ccctaaggaa tgggttgcgg
     2701 tcaagaccca ctttggctcc gagggcgctc acagggtggt gaggccggtc tttctgagaa
     2761 aggtggtgga gggtcttaag gatgtggggg caaggccgtg tgtggtggat acagtaagga
     2821 tcaagggcct tgattatctt gaggtcgcca accaaaacgg cataaatcac ctttctgtgg
     2881 gagcgcctgt gctacttgct gacggcatct acgggaatga caatatcatg gtcagggcag
     2941 gggagatcct gggagagata gctgtggcgt ctcttatcca cgacattccc gcaatggttg
     3001 tctgcagtca tgtgaaagga cacataaatg cagggtacgc gggtgctatc aagaatcttg
     3061 ccatgggggg agtaagtgcg tcccatcgtc attgcgggtg gaagtgcggg agaggggcca
     3121 tgcataccat aggtgagggc gcccttgtat ggaacagtga acaatgcgag ttgtgttacc
     3181 agtgcgcgga ggtgtgcccc ttggatgcca ttgtatttga agagggtgat gagcttgtct
     3241 ggaatggaga tacctgctgg aggtgcggca ggtgcacaag ggtctgtcag aatgaggccc
     3301 tccatcttcc cggggatgat gagaggtttc tccgctccct tgcagaggca gcaaaggcgg
     3361 tcctgggcac ttttgatccc ctgaagatca tctatgtcaa ttttcttagc gaaattcagc
     3421 ctgagtgcga ctgtatgccc atagcggatg tccctgtcat ccaggataag ggaatcttag
     3481 tgtctgatga tttggtagcc gtagaacagg ccagcataga catgctccgc aattcggaac
     3541 ccctgcccga atctgcggcc agcaaagaaa aacttggctc cgacagagat atccttttgg
     3601 gtctgcacaa aaaaaactat ttaattcaag tagatgaggc tgaaaggctg gggcttgggt
     3661 caagaactta tgagctcatt gagatacccc aaaaagaaaa gtagctgctg ttcgtgggaa
     3721 ctgaacagca gcttagggga ggtaacgagg cgctaaatat tagcactttt ataattacag
     3781 aaaagaaacg gtttgtcaag taaaaattta gttattctgt taatggtaag tttagggtgg
     3841 aagtgctgag gaggaacctg actttttaaa tgatttaagg acattagagg actaggataa
     3901 ctcgttatgt tctgtattcc gcgtttatcc ggatatagtc ttctgtgagg tcgcaggtca
     3961 gcactctgga cgacgaactc cccaggcgca ggtccacggt tatggtcacc tccttccctc
     4021 tgattatgcg ggcagcctcc atgtcctttc cctgggagat cccgtttttt actactctca
     4081 ccttcccgaa atagatgtct gttctcgtct ccctaatggc gatccccgag gagcccatag
     4141 ctgccataat cctcccccag ttagcatcat tgccgtaaag ggctgtcttt acaagggggg
     4201 aattggcgat tgaaagtgca gccttcttcg cgtcccattg tttccgagcg ttcttcacca
     4261 caacctcgac aagctttgtt gccccttcac cgtccttgac aatcagtctg cctaattcat
     4321 aagtgatatc agaaagagct gatgagaatc tcatgaacgc tgcggtattc ctcttgatcg
     4381 gctcattttc cgccatgccg ttggccatga taaggaccgt atcgtttgtg gacatatccc
     4441 cgtcaatggt tatgctatta aaagaggttt ccaccgtgtc cttcagggcc ttatcgaggg
     4501 cgcctttgcc aatcttgagg tcggtcatga taaaacaaag catagttgcc atatgcggac
     4561 aaatcatccc cgcaccctta catattccca cgagggtgcc agttttgctg ccaatcctta
     4621 tcctcctcgt caccacctta gggaacgtat ccgttgtcat gattgcggca gctgcgtcat
     4681 ccggtgtgga ttttccgaga ccagttacaa gttcaggaat cttcgccctc accctctcca
     4741 ttggcattgg tgtaccaatg acccctgttg agcatacgta gaccttgtct tgttccaggt
     4801 tcagtccctt tgcgatcagc ctggtcattg ccagagcgtc gcgcattccc tgcttccctg
     4861 tgcatgcgtt ggcatttccg ctgttcacga taattgcctg ccccttaccc gctctgatgc
     4921 gttgcatatc caacttcacg ggtgctgcct ttacttgatt cgtggtgaac atgcctgaca
     4981 taatggcctc acgcggtgaa taaataagga ggaggtcttt tttccccggc ttttttatgg
     5041 cagcctccac agtggagaag aaaaaacctt caggggttat tatgtttgtc ggagacttct
     5101 tattattcat tgctaataaa ttacctcgca tacttttttt ttgcaataga attaagtttt
     5161 cctcgtggtt cggtaatgca tagccaggag actgatattc ggataatggc tcaacactcc
     5221 c
//
LOCUS       DATKOU010000133         5414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1708702, whole genome shotgun sequence.
ACCESSION   DATKOU010000133 DATKOU010000000
VERSION     DATKOU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708702"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="rnc"
                     /locus_tag="VMU21_06095"
     CDS             complement(<1..705)
                     /gene="rnc"
                     /locus_tag="VMU21_06095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HUO77133.1"
                     /translation="MPVSSSENLEQLERDLGYSFHRRDLLLEALTHKSFHHENPSHIP
                     SHNERLEFIGDSVLGLVIVELLFHDKKGYTESEMSEIKSYLVRGSLLSEIAHGMGLGR
                     YLRLGKGEEETGGRNKRSLLANAMEAVFGAIYLDGGYGAAQESILRLFKERIVSVLSS
                     GQYHNYKTDLQEESQMRFGVLPEYHIIHQEGEEHRKIFTVEVFIAGRRLGMGSGKNKK
                     EAQTAAAKEAIERLHTG"
     gene            complement(668..1915)
                     /gene="fabF"
                     /locus_tag="VMU21_06100"
     CDS             complement(668..1915)
                     /gene="fabF"
                     /locus_tag="VMU21_06100"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HUO77134.1"
                     /translation="MEKRRVVVTGLGLITPLGIGVEPSWQGLIEGRSGINTITTFDSS
                     DLPVHIAGEVKGFDPLLYIEPKEVKKMDRFIHFAVAASTMAVDDSGLTITHRNAARVG
                     VIIGSGMGGLPAIEHYHKVYLEKGYRRITPFFIPMLIINLASGQVSIKFGAKGPNSAV
                     ATACATGTHAIGDAFRIIQRGEADVMIAGGTESCITPMAVGGFAVMKALSTRNKEPEK
                     ASRPFDTYRDGFVMGEGAGVLILESLENARERNARIYAEIMGYGMTSDAYHITAPAPE
                     GEGAASCMALTLRDAGVSPEVVDYINAHGTSTKYGDELETKAIKTVFGAHAYALKVSS
                     TKSMTGHLLGAAGGIESVISVLSIHHNTVPPTTNLENPDPECDLDYVPHIPKTLDVNY
                     ALSNSFGFGGTNACLLFRKFRAA"
     gene            complement(1931..2161)
                     /gene="acpP"
                     /locus_tag="VMU21_06105"
     CDS             complement(1931..2161)
                     /gene="acpP"
                     /locus_tag="VMU21_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUO77135.1"
                     /translation="MVEEKVKEIIAKQLGVNPSEVTPEASFVEDLGADSLDTVELVMA
                     FEEAFNIEIPDEDAEKIAKVKDAVEYIKKKMA"
     gene            complement(2260..3000)
                     /gene="fabG"
                     /locus_tag="VMU21_06110"
     CDS             complement(2260..3000)
                     /gene="fabG"
                     /locus_tag="VMU21_06110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HUO77136.1"
                     /translation="MGFKEQVALVTGSARGIGRAIAEALARKGVALAIADVNLDEAKA
                     TSADLAKNGVKTMALRIDVSNSREVITAFQEILKEFGRLDILVNNAGITRDSLLLRMK
                     EEDWDAVIDVNLKGVFLCAKEAVKVMAKQRYGRIVNIASVVAFMGNPGQANYSASKAG
                     IVGLTKTLAKEYASRGITANAVAPGFITTAMTDALPDNVKEEMKKAIPMARFGTIEDV
                     ANAVVFLASPSSGYITGQVIHVNGGMYM"
     gene            3514..4599
                     /locus_tag="VMU21_06115"
     CDS             3514..4599
                     /locus_tag="VMU21_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HUO77137.1"
                     /translation="MFLLYTITLIPGDGVGPEIAEATRRVVEATGVAIKWEIQDAGEE
                     VYRNEGTPLPQRVIESIRKNRIAIKGPVTTPVGTGFRSVNVTLRQTLDLYCCLRPCKS
                     FKGARTKYDDIDLIIVRENTEDLYAGIEFQKSSHNTSRLIDLVKEATGKTISQDSGIS
                     VKAISVAASERIVRFAFEYARRNGRKKVTAVHKANIMKYSDGLFLDVARHVAGQYPDI
                     EFEDRIIDNMCMQLVQKPELYDVIVLPNLYGDIVSDLGAGLIGGLGLAPGANIGSTMA
                     VFEPTHGSAPKYKGLNKMNPIAMILSGVMMLIHLGEKEAALRLERAVASVIEEGTHVT
                     YDMKPAPDDPTSATTSRVADAIIEKLH"
     gene            4671..>5414
                     /locus_tag="VMU21_06120"
     CDS             4671..>5414
                     /locus_tag="VMU21_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HUO77138.1"
                     /translation="MWFDIFLISIFIVLNGLFNAAEIAVVTARRSRIKQLIEEGNKNA
                     EQLLQFKEEPNRFLATIQIAITLGSVLASAIGGAAAIQHIKPVISEMPYRPIAASSEA
                     ISIGVVSIVITYFAMVFGELIPKSVALSHPEEVGLRIARPVEAFSRFATLIVKLLTFS
                     ANLLLKPFGRKTFTERAYVTEEEVKMLIMEGGEQGVFEPTEQELIHSVFEFTDTSARE
                     VMVPSTRMVNIALNMSVDEIKFTIAEEQFS"
BASE COUNT         1312 a         1407 c         1308 g         1387 t
ORIGIN      
        1 tcctgtatgg agcctctcga tggcctcttt agccgctgcg gtttgtgcct ctttcttgtt
       61 ttttccgctc cccatgccta acctcctgcc tgcgatgaat acctccaccg tgaatatctt
      121 gcgatgttct tctccctcct ggtgtatgat atgatactca ggcaggaccc caaaccgcat
      181 ctggctttcc tcctgcaggt ctgtcttgta attgtggtac tgcccggagg ataatacgga
      241 cacgatccgt tccttgaaaa gcctgaggat tgactcttgc gcagccccat atcctccgtc
      301 aaggtagatc gctccaaata ccgcttccat ggcatttgcc aggagggatc tcttatttct
      361 tccgccggtc tcttcctccc cctttccgag cctcagatat cttcccagtc ccatgccatg
      421 ggcgatttcg gagagaaggg acccccttac gagatatgac ttgatttcgg acatctcgga
      481 ttctgtatag ccttttttgt cgtgaaacaa aagttcaacg atgacaagac caagtacgga
      541 atcgccgata aactcaagac gctcgttatg tgacgggatg tgggaaggat tttcgtggtg
      601 gaacgacttg tgggtcaggg cctcaaggag cagatcccgg cggtgaaagg aatagcccag
      661 gtccctttca agctgctcta aattttctga agaggagaca ggcattcgtc ccgccaaaac
      721 caaaggagtt tgaaagggcg tagttcacat caagagtttt cggtatatgg ggaacgtagt
      781 cgagatcgca ttccgggtcc gggttctcaa ggttcgtcgt gggaggaacc gtgttatgat
      841 ggatgctgag gacgcttatg acgctctcaa taccgcctgc cgcaccgagc aaatgtccgg
      901 tcattgattt tgttgagctc accttcagtg cataagcatg tgccccgaat actgtcttta
      961 tggcctttgt ttccagctca tcgccatatt ttgtggacgt cccgtgggca ttgatgtaat
     1021 ccaccacttc aggagaaacc cctgcgtccc gcaacgtgag ggccatgcac gaagcggcgc
     1081 cttccccctc aggagcgggg gcggtgatgt gatatgcgtc actggtcatc ccgtaaccca
     1141 taatttctgc atagatccgg gcgttcctct cccttgcatt ctccagtgac tcgaggatca
     1201 aaacccctgc gccttctccc atgacaaatc cgtcccgata cgtgtcaaac ggcctgctcg
     1261 ccttttccgg ctccttattt cttgtggata acgccttcat cactgcgaat ccacctaccg
     1321 ccatgggggt tatgcaagat tcagttcctc ccgcgatcat cacatcagct tcgcccctct
     1381 gtatgatcct gaatgcgtcg ccgattgcat gggtcccggt ggcgcaggca gtggccacag
     1441 cggaattggg gcctttggcc ccgaatttta tcgagacctg acccgaggca agattgatga
     1501 tgagcatggg gataaagaaa ggagtgattc tccggtaccc cttctcaaga tatactttgt
     1561 gatagtgctc aattgcgggg agtcctccca tgccagagcc gataatgact cccacccttg
     1621 ccgcgttacg gtgagtgatg gtgagtcctg agtcatccac ggccatggtc gatgcggcca
     1681 cagcaaagtg gatgaacctg tccatcttct tcacttcctt cggttcgatg tacaggaggg
     1741 gatcgaaacc cttcacctcg cccgcaatgt gaacaggaag gtctgaggaa tcgaaggtcg
     1801 tgatagtatt gattcccgac ctgccctcta taagcccttg ccatgagggc tccaccccaa
     1861 tccccagggg ggtgatgagt cccaggccag ttaccacaac tctccgtttt tccatgattc
     1921 cctgaaggcc ttaggccatt ttcttcttta tatactctac ggcgtccttt acctttgcga
     1981 ttttctcggc atcctcatcc ggaatctcga tattgaatgc ctcttcaaat gccatgacca
     2041 gttccaccgt atccagagaa tcagctccga gatcctcgac aaatgatgcc tcaggggtta
     2101 cctctgaagg atttacccct agctgtttcg cgataatctc ctttactttt tcttcaacca
     2161 tcattgcacc tccttaattc aaaattgata tgtaaactgc aaaaggtaaa actttcttac
     2221 caataggctc tttccggaac cgtacagggt catgggtgct tacatataca taccgccatt
     2281 tacatgaatc acctggccag taatataccc ggaggaagga gacgcaagaa acactaccgc
     2341 atttgccacg tcctcaatgg ttccaaacct tgccatgggg atcgcctttt tcatctcctc
     2401 tttcacattg tcggggaggg catcggtcat tgcagtggtg ataaagccgg gagcaactgc
     2461 attggcggta attcccctgc tcgcatattc ctttgcgagg gtcttggtca gcccgacgat
     2521 acccgccttt gacgcagagt agtttgcctg tcccggattg cccataaagg caaccacaga
     2581 agcaatattg acaatccttc catacctctg ttttgccatt acctttaccg cttcctttgc
     2641 gcagaggaaa acccccttga gattcacatc aatcaccgcg tcccagtctt cctccttcat
     2701 ccttaataaa aggctgtcac gggttatccc cgcattattt acgagtatat ccaacctccc
     2761 aaactctttc agtatctctt ggaatgcagt tatcacctcc cttgaattcg atacatcaat
     2821 cctgagcgcc attgtcttta cgccgttttt cgccaaatcc gcggaggtgg cctttgcctc
     2881 atcgaggttg acatcagcaa tcgccagggc cacacccttg cgggcaagtg cttccgctat
     2941 cgcccttcct atacccctcg cacttcccgt aacaagtgca acctgttctt taaaccccat
     3001 cagtacccct ccccatgaaa gacggctata attttaacag agaaatcttt cattttccag
     3061 cttttttatg aattagcaaa atctcattga aaaacacgag attgttcaca gtcgcactgc
     3121 caggaatccg ttgcaacaat caaggaatgg acctgtactc tgggcgtctg aaaacgagtg
     3181 taacggagaa gaccctcaaa atcgaaactt ttggggaggg gctggacagg aggcatctcc
     3241 ctgtatcaac atattgtctc ttatttgttt gagaaccgta gagcacgcaa aaagataaat
     3301 ttgcaatacc tgtcaggttt ttggttataa tattcaccaa attatccgca ggaaagggtg
     3361 ggtgtgcatg catcattata ttcatactca tgtgacaaag gagagccctg agcctttgct
     3421 gtatacaaac atgcttacac taacactatt gcaagagagc gggccgggtt gtattacgcg
     3481 ctatgctgac gccagcagaa cgatccttat agggtgtttc tcttgtatac aataacgtta
     3541 atacctggtg acggggtggg gcccgaaatc gcagaagcca ccaggcgtgt ggtcgaagca
     3601 acaggagttg ccatcaaatg ggagatacag gatgcaggag aagaggtata ccgaaatgaa
     3661 gggacaccac tcccccagag ggtgatcgaa tccatcagga agaacaggat tgcgattaag
     3721 ggaccggtga cgacccctgt gggcacaggc ttcaggagtg tgaacgtcac cttgcggcag
     3781 accctggatc tctattgctg cctcagacca tgcaagagtt ttaagggtgc aagaacgaag
     3841 tatgatgata tagacctcat tatcgtcagg gagaatacag aggatctcta cgcaggcatt
     3901 gagttccaga agtcctctca taatacctca agacttattg atctggtgaa agaggccacg
     3961 gggaagacaa tcagtcagga ttcgggaata agtgtcaaag cgatctctgt ggcggcaagc
     4021 gagcgcattg tgaggtttgc ctttgaatat gcaaggcgaa acgggcgaaa gaaggtgacc
     4081 gcagtgcata aggcaaacat tatgaaatat tctgacggat tgtttctgga tgtggcgcgg
     4141 catgtggctg gccagtatcc tgacattgaa tttgaagacc ggataatcga taatatgtgc
     4201 atgcagcttg tccagaaacc cgaattgtat gacgtgatcg tgctccccaa tctgtatggc
     4261 gatatcgtgt cagaccttgg agctggtctt atcggcggac tgggtcttgc gccgggtgcg
     4321 aacatcggca gtaccatggc ggtgtttgaa cccactcacg gcagtgcacc aaagtacaag
     4381 ggcttgaata agatgaaccc tattgctatg atactctcag gggttatgat gcttattcac
     4441 cttggagaaa aagaggcggc actacgattg gaacgggcgg tcgcttcggt gatagaagaa
     4501 ggcacccacg tcacctatga tatgaaacct gctcctgacg atccaacctc tgcaacgact
     4561 tcacgggttg cagatgccat tatagaaaaa ttacactgac catttgcatg gaagctgaga
     4621 gttcacccaa taaagagctt cactcacaca cacagtgctg aaaggtcccc atgtggtttg
     4681 atatcttcct catcagtata ttcattgtcc tcaatggcct ttttaatgct gcggagattg
     4741 cagtggtgac cgcacggagg agccggatca agcagctcat tgaggagggc aataagaatg
     4801 cggaacaact ccttcaattt aaggaagagc ctaatcgttt tcttgccacc atccagattg
     4861 ccatcaccct gggcagtgtg ctcgcctcag ccatcggtgg tgcggcagca atccagcaca
     4921 taaaaccggt tatcagtgag atgccttata gaccaatcgc tgcatcaagc gaggccattt
     4981 ccatcggcgt cgtaagtatt gtcatcactt attttgcgat ggtctttggt gagctcatcc
     5041 caaagtccgt ggcactttcg catcctgagg aggtgggact cagaatcgca agacccgttg
     5101 aggcattctc gcgtttcgcc accctcattg tgaagctcct caccttcagt gcgaacctgc
     5161 tgctcaaacc attcgggaga aagaccttta ccgaaagggc atacgtgacc gaggaggagg
     5221 tcaagatgct tattatggag gggggcgagc agggtgtctt tgaacccacc gagcaggagc
     5281 ttattcacag cgtgtttgaa ttcacggaca cgtcagccag agaggtcatg gttccctcca
     5341 cgcggatggt gaatatagct ttgaatatgt ccgtggatga aataaaattt actatcgctg
     5401 aagaacagtt ttcc
//
LOCUS       DATKOU010000134        10655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2482800, whole genome shotgun sequence.
ACCESSION   DATKOU010000134 DATKOU010000000
VERSION     DATKOU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482800"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VMU21_06125"
     CDS             complement(<1..397)
                     /locus_tag="VMU21_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HUO77139.1"
                     /translation="MDDLKKFVGNDCFARHLGIEVLEYSEGKARARMEIHPHHLNSAG
                     TLHGGAIFSLADAVFSVASNSHGTLAVAINVGIAYFKAVKSGTLTAAAEEVSLNPKLA
                     TYLIRVEDEKGDAIALFQGTVYRKKDRLGD"
     gene            complement(474..1508)
                     /locus_tag="VMU21_06130"
     CDS             complement(474..1508)
                     /locus_tag="VMU21_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HUO77140.1"
                     /translation="MKLRFTRTNGLLDTAIDQLIELAAGIHSPELIREMIIAALMAGQ
                     EEKEDKADLKLMNTTLKEMRFTSKVFGPYKNVRKITVFGSARTKPDDEVYTMARLLGR
                     RIAEAGYMVITGGGPGIMQAVNEGAGPKHSFGVNIRLPFEQKPNPVLEGNPRLIVYKY
                     FFNRKVAFIKEAHAIVLFPGGFGTLDEAMETLTLVQTGKRDPLPLILVDEPDGTYWSH
                     YLQFFREKLLKEGYISDTDFDLFECVDSVEDVMERINHFYRRYHSLRYVEGQLVMRLT
                     SPLDAGSIKRLGEGFSDILIPGGGIVHSVSLPAEADEPDLAGLPRLIVDFDRKNFGRL
                     RSLINAINSF"
     gene            1601..1858
                     /locus_tag="VMU21_06135"
     CDS             1601..1858
                     /locus_tag="VMU21_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77141.1"
                     /translation="MPDVIARQKDNFLPREIPPVVQCDIMSFTKPVLVPKNVIYEFFL
                     LGLVSSVAFRAILRRRLLAPAKRAPGMRDTRMSPAGREMPL"
     gene            1855..2154
                     /locus_tag="VMU21_06140"
     CDS             1855..2154
                     /locus_tag="VMU21_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77142.1"
                     /translation="MMFEQRKKERYEFSGDKVDFTLTPFAQEELYSAGVMNFSETGIC
                     LLSSVRLSLGQEITIRDFMSFSSRSAVVIWVEKYTDIFYLNKSDEVLFRIGLLFT"
     gene            2254..4095
                     /locus_tag="VMU21_06145"
     CDS             2254..4095
                     /locus_tag="VMU21_06145"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HUO77143.1"
                     /translation="MANKEQLEKIARLIRYFSLVSTTEAGSGHPTSSLSAADLMTGLM
                     FGGTFRFDVDRPGHPNNDRLIFSKGHASPLFYALWAATGKVTEEELMTMRKFGSPLEG
                     HPTTAFPYTEASTGSLGQGLSVGLGMAMNAKYIDRLPYRTYVLLGDSEMAEGSQWEAL
                     EIASYYQLDNLMGIMDVNRLGQRGETMYGHDLSVYEQRVSSFGWEPFVIDGHSMSEIL
                     AAYEKALTVRRKPVMLIAKTIKGKGISFIEDKNGWHGKALKKEELVRALEELGPVDTS
                     VRGEITKPADLKPETPSPVRAGVAAYSPDKPIATRHAYGNALARIFPQFPDIVVLDAE
                     VSNSTFAEIFRDAHPGHFFEMYIAEQNMVGAALGLSRRGKIPFVSTFAAFLTRAFDQI
                     RMSQYSDANIKFCGSHAGVSIGEDGSSQMGLEDIAMFRTLLHSVVLYPSDAVSTEKLV
                     ERAADHKGIVYLRTTRKDTPIIYASDEDFPIGGSKVLRSSNEDAVTVVAAGITLHEAL
                     AAHDTLRQEGIFIRVIDLYSVKPLDKATLKIAARVSQAIITAEDHFAEGGIGEAIRSV
                     MSEIDIPIYCLSVQKMPMSGKPDELLEYEGISKNAIMKKVKEILGNG"
     gene            4146..4598
                     /gene="rpiB"
                     /locus_tag="VMU21_06150"
     CDS             4146..4598
                     /gene="rpiB"
                     /locus_tag="VMU21_06150"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HUO77144.1"
                     /translation="MKVALGSDHGGFLLKEVMVGFLAGEGHQVLDLGAHTPDPSDYPD
                     FARAVAEAILSKKADRGILICGSGVGASVAANKFPGIRAAVCHDTFSSHQGVEDDEMN
                     VLCLGERVIGPELAKEIAVSFLSARFSGAERHIRRLKKVKEIEKEQMK"
     gene            4657..5301
                     /locus_tag="VMU21_06155"
     CDS             4657..5301
                     /locus_tag="VMU21_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77145.1"
                     /translation="MKVVPAILAEKFDDFVKRLRQAESFTDYVQVDLMDGVFVETRSF
                     PPERINSVEMQLFFEVHLMAEDPLGFMNGMEHPGLKKVIFHCETGVNHLDLVSTIRGR
                     GLSPGVAIKPETGLAQCRGIAEHVDTLLFLTVDPCCYGSPFKPEVLHKIVEARRLFPA
                     KLISVDGGVSLDNLKDFFDAGVDYACVGSRIFLQKDPGESYRLFAHKMKELERR"
     gene            5298..6086
                     /locus_tag="VMU21_06160"
     CDS             5298..6086
                     /locus_tag="VMU21_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HUO77146.1"
                     /translation="MRSLMYLTLFLALISLLTGGIASANETKGKEQIGIEEKLGQRIP
                     LDASFRDETGKEVLLKEVVGKPIIVSLVYLKCAHTCPLLLGALAEVLGRLDLKPLKDY
                     SVLTISFDEEDTPDIARDKKRNYLQAINHPFPESAWRFFTGDRENIKKFTDAVGFNFR
                     REESGFAHPVALIILSPDGRIVRYLYGRTFLPFDLTMAITEADKGKIGLSRGVLLYCF
                     SYDQPGRRYVFNILKVFGTVMIVSLVSFFLYLTVTGRRSQGGPK"
     gene            6083..7690
                     /gene="ctaD"
                     /locus_tag="VMU21_06165"
     CDS             6083..7690
                     /gene="ctaD"
                     /locus_tag="VMU21_06165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HUO77147.1"
                     /translation="MNARENSPLSVSFYDDSGRYRGLYSWLFTTDHKRIGILYLISVL
                     SFFAVGVTLGLLMRLNLIDPGRFISERTYNSLFTVHGVIQIFLFIIPAIPTTLGNFFL
                     PLLIGARDVAFPRINLLSWWLFILGGLIVVLSIFTGGGAPDTGWTFYAPYSIRTASNV
                     SLALFGVFILGFSSILTGLNFVTTIHRLRAPGMKWFRMPLFVWSLYATAWTQILATPI
                     LAIAVLLLVLERSFGIGFFDPTKGGDPILFEHLFWIYSHPAVYIMILPAMGVVTEIFP
                     VFARKTVFGYKAIAFSSLAIAFFGYLVWGHHMFTSGMSDTSRIIFSLITFLVAVPSGV
                     KVFNWIATLYKGSIEVYSPLLFSLAFIFLFSIGGLTGLINGTLSLNLYIHGTYFIVAH
                     FHYTMFGGAGFGFFAGLHYWFPKMFGRMYNERAARTACFIMFIGFNILYFSMFILGLQ
                     GMPRRYFQYLPQFRTNHIISTIGSWIFVAGLLLMFGNLIWSLRKGKKAPGNPWGGKTL
                     EWQVPSPPPRENFEEIPVITHGPYRFD"
     gene            7698..8318
                     /locus_tag="VMU21_06170"
     CDS             7698..8318
                     /locus_tag="VMU21_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HUO77148.1"
                     /translation="MEKTETAHAVHTRDIEGAKMGMWVFLFTEMLLFGGLFLLYSVYR
                     SKYSAEFHGSAAELDIVLGTLNTVILLTSSLTMVLSISALRKGHRGLSSLFQGMTILL
                     AIIFLVNKYLEWSTHIHHGIYPDSPALLAMERGQIIFFSLYYVMTGIHGLHVLIGAGV
                     IAGTLVFTTQGKLGPDNFVMLENTGLYWHFVDIIWIYLFPLFYLIV"
     gene            8337..8630
                     /locus_tag="VMU21_06175"
     CDS             8337..8630
                     /locus_tag="VMU21_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HUO77149.1"
                     /translation="MSTEQETQLTATRYRKYVYVWLSLIVLTSLTVSVARLHLTSYAI
                     LIAILIATTKAGIVVTFFMHLKNEPLILRLMLFVALLAFTLIALLTFSDVWYR"
     gene            8638..9537
                     /gene="coxB"
                     /locus_tag="VMU21_06180"
     CDS             8638..9537
                     /gene="coxB"
                     /locus_tag="VMU21_06180"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HUO77150.1"
                     /translation="MFIPGASNSARHVDATFAVILAIEVFLLFAVTAAMIFFVIRYRK
                     KKNPRSENVEGGMLLEIAWTAIPTVLVLIMFYIGLKSFILIRKVPEGAMPVQVTARQW
                     SWQFTYANGKQSSTLAVPLNRPVKLILRSQDVLHCLYIPAFRIKEDCVPGLETYLWFT
                     AKETGSYDIFCTEYCGLGHSDMLSKVVVTSAKEFDAWYAGEGEKGGLGLTVLESRGCL
                     GCHSMDGSKKIGPTFKGLYGSQVTVLTSGKERTVTSDEEYLRRSILEPGADIVKGYQN
                     IMPALPVTKEELDAIIQYLKTLK"
     gene            9558..10418
                     /locus_tag="VMU21_06185"
     CDS             9558..10418
                     /locus_tag="VMU21_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HUO77151.1"
                     /translation="MPLLVYSELCKVRISLFSAFSALTGLLLAAREFRPEAVVLVAGV
                     FLLACGSSALNQYQERKTDVLMPRTEGRPIPSGRMEPSHALFFSVMLISLGSLTLLLT
                     GRAVAPVLGLLAVIWYNGLYTYLKRKSAFALIPGALVGAIPPAIGWVTGEGTFSDPGL
                     IALCFFFFLWQVPHFWLLILSYGEEYRNAGLPSLTAIFTRTQLLRIIVNWIFATAVSC
                     LFLSISGMVLTPLINYMLLGVSLWLMATAIRLLIQGGEVTSTYTSTFRRINTYMFLVM
                     TLLSVDKLIV"
BASE COUNT         2443 a         2928 c         2698 g         2586 t
ORIGIN      
        1 ggtctcccag tctgtccttc tttctgtaca ccgtaccctg gaagagcgca atggcgtcac
       61 ctttctcatc ttccaccctg atcaggtagg tggccagttt ggggttaagg gaaacctctt
      121 ccgctgctgc tgtgagggtt ccgcttttta ctgccttgaa ataggctatg cccacattga
      181 ttgccactgc caatgtcccg tgggagttgg atgccacaga gaagaccgca tcagccaggg
      241 aaaaaatcgc cccgccgtga agggtccccg cgctattgag atggtgaggg tgaatctcca
      301 tcctcgcccg cgcctttcct tccgagtatt ccaggacctc gatccccaga tggcgagcga
      361 aacagtcatt gccaacaaac tttttcagat catccatatt cattcctcct tcaaaagata
      421 atcacgcacc tcgcgaacat ctctgagcat gtggataaga agtcccgttc cggtcagaaa
      481 ctgttaatgg cattgatgag gctcctgagc cttccgaagt tcttgcggtc aaagtccacg
      541 atgagtctgg gtagcccggc aaggtccggc tcatcggctt ccgcgggaag agagacagag
      601 tgaactatcc cgcccccggg aatcaggatg tcggaaaaac cttctcccag tctcttgatt
      661 gaccccgcat ccagaggtga ggtgaggcgc attaccaatt gcccctccac atatctcagg
      721 ctgtgataac ggcggtagaa atggttgatc ctctccatca cgtcctccac cgagtcaacg
      781 cattcaaaaa gatcgaagtc ggtatcactg atatatccct ccttcaagag cttttcccta
      841 aaaaactgga ggtaatggga ccagtaggtg ccatcaggct catccaccag aatgaggggc
      901 aaaggatctc tcttcccggt ttggacaagg gtgagggtct ccattgcctc atccagggta
      961 ccaaagcctc cggggaaaag gacgatggcg tgagcctctt tgataaaggc caccttacga
     1021 ttgaaaaaat atttatacac tatgagacgg gggttcccct cgagcacagg gtttggtttc
     1081 tgctcaaaag ggagcctgat gttcacccca aaagagtgtt tcgggcctgc accttcattc
     1141 actgcctgca tgataccagg gcctcctccg gtgattacca tgtaacccgc ttcagcgatc
     1201 cttctcccga gaaggcgtgc catcgtatat acctcatcgt caggttttgt tctggcagaa
     1261 ccgaatacgg taattttcct cacgttctta taagggccga atacctttga ggtaaaacgc
     1321 atttccttca gcgtggtgtt catcaacttc aggtccgcct tatcctcttt ttcttcctgg
     1381 cctgccatga gtgcagcgat aatcatctcg cggataagct ccggggagtg gatgcccgcg
     1441 gccagctcaa tgagctgatc aatagccgta tccagtaatc cgttggttct cgtaaaacgc
     1501 agtttcatac cacccctatc tttttgtgcc attctaccaa tctgcgccga catgttcaag
     1561 aagataagac cggccagata acttggcagt cgtggagcgc atgcctgatg tgatagccag
     1621 gcagaaggac aacttcctcc cccgggaaat ccctcctgtg gtacaatgcg atattatgtc
     1681 attcacaaaa ccggtactgg tcccaaaaaa tgtaatttac gagtttttcc tgctcggcct
     1741 ggtctcgtcg gtggccttca gggcaatctt acggaggagg cttcttgctc ctgcgaaaag
     1801 agctccgggg atgagagaca cacgaatgtc ccctgcagga cgggagatgc cactatgatg
     1861 tttgaacaga ggaaaaaaga acggtatgag ttctcgggtg ataaagtgga ttttacgctg
     1921 actccctttg ctcaggaaga gctctacagt gccggtgtaa tgaatttcag tgagacaggg
     1981 atatgtcttc tgtcatcggt ccgcctctct ctgggacagg agatcaccat cagggacttc
     2041 atgtcctttt cttcccggtc agcggtagta atctgggtgg aaaaatatac cgacatcttt
     2101 tatctcaaca aatccgatga ggtcttgttt aggataggcc tcttgttcac ataagttctg
     2161 tgcatgccct ttcccctccc tgccctcaag ttgacaacac ccttaaactt gttagaattg
     2221 aggtgatgcc ggcacagaaa agaaggagct cttatggcaa ataaagaaca gttagagaaa
     2281 attgcacgac ttattcgtta cttcagtctt gtctctacca cagaggcagg atcgggacat
     2341 cccacctctt ccctctctgc agctgacctg atgaccggcc tcatgtttgg cgggaccttc
     2401 aggttcgatg tggacaggcc gggccatcca aacaatgacc gcctcatttt ttccaaaggc
     2461 cacgcatcac cccttttcta tgccctgtgg gcagctacgg gtaaagtcac cgaagaggaa
     2521 ctcatgacca tgcgaaaatt cggaagcccc ctggaaggcc accccaccac cgcctttccc
     2581 tacaccgagg cctcgacagg ttccctcgga caggggctct cggtcggtct cggcatggcc
     2641 atgaacgcga agtatataga caggcttccc tacaggacct atgtgctgct gggagacagc
     2701 gagatggctg agggttccca atgggaagca ctcgagatcg cctcatatta tcagctagac
     2761 aatctcatgg gcataatgga cgtgaaccgg ctcgggcaga ggggtgagac catgtacggc
     2821 catgatctct ctgtgtatga acaaagggtt tcgtcctttg gatgggaacc gttcgtcatc
     2881 gacgggcact ccatgtcaga aatcctggct gcgtacgaaa aggccctcac tgttcggaga
     2941 aaaccggtca tgctcatcgc caaaacaata aagggcaagg gcatctcgtt tatcgaagac
     3001 aagaacggct ggcacggcaa ggccctcaaa aaagaagaac tggtccgcgc acttgaggaa
     3061 ttgggtcctg tggatacgtc tgttcgtggt gagatcacga aacccgctga tcttaaaccc
     3121 gaaacaccct ccccggtcag ggcaggtgtc gctgcgtatt cacctgataa gcccattgct
     3181 acacgacacg catacgggaa tgccctggcg cgtatctttc cccagttccc tgatattgtg
     3241 gtccttgatg ccgaggtgag caattccact tttgcagaga tattcaggga tgcacatcca
     3301 ggacactttt tcgagatgta tatcgctgag cagaacatgg tgggagctgc attaggtctg
     3361 tcgcgcagag ggaagattcc ttttgtctcg acttttgccg cgtttcttac acgcgccttt
     3421 gatcagatac ggatgagcca gtactctgac gcgaatatca agttctgcgg ttcccacgcc
     3481 ggagtctcca taggcgagga cggttcatcc cagatgggac tcgaagacat cgccatgttc
     3541 aggaccctcc tgcacagtgt ggtgttgtat ccctctgacg cggtatccac cgagaagtta
     3601 gtggaaagag cggcagacca taaaggaatc gtgtatctgc gcaccaccag aaaagacact
     3661 ccaatcatct acgccagtga tgaggatttc ccgatagggg gaagcaaggt actgaggagc
     3721 agcaacgaag atgccgttac cgtggttgct gcaggaataa cgctccacga ggcactggct
     3781 gctcacgaca ccctgagaca ggaagggatt tttatccgag taatcgacct ttacagcgtc
     3841 aagccactgg ataaggcaac ccttaagata gctgcccgcg tctcccaggc cattattacg
     3901 gcagaggacc attttgccga aggcggcatt ggtgaggcca tcaggagcgt catgtctgaa
     3961 atcgacatcc ccatttattg cctgtcagtg cagaaaatgc ccatgagcgg gaagccggat
     4021 gagctcctgg aatacgaagg gatttcgaag aatgccatta tgaaaaaagt gaaggagata
     4081 ctgggaaacg ggtgaatgaa agcaaaccat gcaatagaga tgaatgagct cagagacctg
     4141 ataccatgaa agtggccctg ggatcggacc atggagggtt tcttctcaaa gaggttatgg
     4201 taggatttct ggcaggggaa ggacatcagg tccttgacct tggtgctcat accccggacc
     4261 cctcagacta tcctgatttt gccagggcag tggccgaggc aatcctcagc aaaaaagcgg
     4321 acagaggcat actcatctgc ggcagcgggg tcggcgcttc agtggccgca aataagttcc
     4381 cgggcatcag ggctgcggtc tgccacgata ccttctcttc ccatcagggt gttgaagacg
     4441 acgagatgaa tgtgctctgc ctcggggaaa gagtgatagg tcctgagctg gcaaaggaaa
     4501 ttgcagtaag ttttctgtcc gcacgatttt ccggagcaga aaggcatatc cgcagactca
     4561 agaaggttaa ggaaatagag aaggaacaga tgaagtgaag tggcacttat tgccttcact
     4621 ggatatcttc ctcaaatgga cacgtaagga gaaacaatga aagtcgttcc cgcaatcctt
     4681 gctgaaaagt ttgatgattt tgtgaagaga ttacgccagg cggagtcttt caccgattat
     4741 gtccaggtcg acctcatgga cggggtcttt gtcgagacaa ggagttttcc cccggaaagg
     4801 ataaactctg ttgagatgca gctcttcttt gaggtacatc tcatggcaga ggaccccctg
     4861 gggttcatga acggcatgga acatccgggg ctgaagaagg tgatattcca ttgtgagaca
     4921 ggcgtgaacc atcttgacct cgtgagtacc ataaggggga gagggctttc tccaggtgta
     4981 gccatcaagc ccgagacagg attggcccaa tgcaggggga ttgcggagca tgttgacaca
     5041 ctgctgtttc tcacagtcga cccctgctgt tacgggagcc cctttaaacc cgaagtcctc
     5101 cataaaatcg ttgaggcgag aagacttttc cctgcaaagc tcatttccgt cgacggaggc
     5161 gtctccctcg acaacctgaa ggacttcttt gatgcaggag tcgattatgc gtgtgtgggg
     5221 agcaggatat tcctccagaa agaccctggg gaaagttaca gactctttgc gcacaagatg
     5281 aaggaactgg agcgtcgatg aggtcgctca tgtaccttac gctttttctt gccctcatct
     5341 ctctgctcac aggcggaatc gcttcagcga atgagacgaa ggggaaggaa caaataggga
     5401 ttgaagagaa actgggacaa agaattcccc tggatgcgtc attccgcgat gagacaggaa
     5461 aggaggtact gctgaaagaa gtcgtgggga agccgattat cgtatccctc gtctatctga
     5521 aatgtgccca cacctgtccc ctccttttgg gtgcccttgc cgaggtcctc ggcaggcttg
     5581 accttaaacc gctgaaagac tactcggtcc tcaccatcag ttttgatgag gaagatactc
     5641 ctgacatcgc ccgtgacaag aaaagaaact acctccaggc catcaatcat ccctttcctg
     5701 aaagtgcgtg gaggtttttc acaggggacc gtgagaacat aaaaaaattc accgacgcag
     5761 tggggttcaa tttcaggagg gaagagagcg gctttgccca tcccgttgcg ctcatcattc
     5821 tttcccctga cggaaggatc gtgaggtatc tctacggcag gacctttctc ccctttgacc
     5881 tcaccatggc catcaccgaa gcggacaagg gcaaaatcgg actcagccgg ggggtgcttc
     5941 tctattgctt cagctatgac cagccgggaa gacgctatgt ctttaacatc cttaaggtct
     6001 tcggtaccgt gatgatcgta tccctcgtct ccttcttcct ctatctcacg gtcaccggaa
     6061 gaagatcaca gggagggccg aagtgaacgc ccgggagaac tctccgctct ccgtaagctt
     6121 ctatgatgat tccgggcggt accggggact ttactcatgg ctcttcacca ctgaccacaa
     6181 gagaatagga atcctctacc tcatttcagt gctcagcttc ttcgcggtag gggtcacgct
     6241 cggcctgctt atgagactta acctcatcga tcccgggagg ttcatatccg agaggaccta
     6301 caattcactt tttactgtcc acggcgtaat ccagatattt ctcttcatca ttcccgccat
     6361 ccccaccacg ctggggaatt tcttcctccc cctcctcatc ggtgcaaggg atgtggcatt
     6421 ccccaggatc aacctcctct cgtggtggct cttcatcctt ggcggactca tagtggtcct
     6481 ctcgatattc accggaggcg gcgcgcccga cacaggctgg accttctatg cgccgtacag
     6541 catacggaca gcgtccaatg tctccctcgc ccttttcggt gtattcatcc tcggcttttc
     6601 gtccatactc accggtctga atttcgtcac caccatccac cgtctcaggg caccgggcat
     6661 gaagtggttt cgcatgccgc tctttgtgtg gtcactctat gccacggcat ggacacagat
     6721 cctcgccact cccatcctcg ccatcgccgt cctcctcctt gtcctggaga ggtcgttcgg
     6781 catagggttc ttcgatccca caaagggagg ggacccgatc ctcttcgagc accttttctg
     6841 gatatactcc catcccgcgg tctacatcat gatcctccct gctatgggcg tggttaccga
     6901 aatattccct gtctttgccc ggaagaccgt cttcgggtat aaggcgatcg ccttttcgag
     6961 cctcgccatc gccttcttcg gatatcttgt ctggggccac cacatgttca caagcggaat
     7021 gagcgacaca tcgaggatca tcttctcgct catcaccttc cttgtggcgg tcccgagcgg
     7081 ggtgaaggtc ttcaactgga tcgcgaccct ctacaaaggc tcgatcgagg tgtactcccc
     7141 gctgctcttc agccttgcct tcatcttcct cttctccata ggcggactga caggcttgat
     7201 caacgggacc ctttcgctga acctctatat ccacggaacc tatttcattg tcgcccattt
     7261 ccattacacc atgtttggcg gcgcgggctt cggcttcttt gccggcctcc actactggtt
     7321 tcccaagatg ttcgggagaa tgtacaacga acgcgctgca aggaccgcgt gcttcatcat
     7381 gttcatcggc ttcaatatcc tctatttctc catgttcatc ctcggtctcc agggcatgcc
     7441 caggagatac tttcagtacc tgccccagtt ccgcaccaac catattatct ccactattgg
     7501 ttcgtggata tttgtcgcag gcctcctcct tatgttcgga aatctgatat ggtccctccg
     7561 gaaggggaaa aaggcgcccg gcaacccatg ggggggcaag acccttgaat ggcaagtacc
     7621 ctcccctccg ccgcgcgaga actttgagga gataccggtg atcacgcacg gtccctatag
     7681 atttgactga gggaagaatg gaaaagactg aaacagctca cgcggtacac acgagagaca
     7741 tcgagggggc gaagatgggc atgtgggtct ttctctttac cgagatgctc ctctttggcg
     7801 ggttgttcct tctctattcc gtgtaccgtt cgaagtacag cgcagagttt cacgggtctg
     7861 cggctgagct cgacatcgtg ctcggtacct tgaataccgt catcctcctt acgagcagcc
     7921 tgaccatggt cctctccatc tccgcgctcc ggaagggaca ccggggcctc tcatcccttt
     7981 tccagggcat gaccatactc ctggcgatca tctttctcgt gaacaagtat ctggaatgga
     8041 gtacccatat ccatcacggg atttacccgg actccccggc actccttgcc atggaaagag
     8101 gtcagatcat ctttttcagt ctctactatg tcatgacagg gattcacggc ctccatgtcc
     8161 tcatcggtgc aggagtgatc gcaggaacgt tggtcttcac cacgcagggg aaactggggc
     8221 ctgataattt tgtgatgctt gaaaacaccg ggctctactg gcattttgtg gatatcatct
     8281 ggatatatct tttccccctt ttctatctca tcgtgtaagg gcatggcgga ggaagaatga
     8341 gcacagaaca ggaaacgcag ctcacggcca cacgctacag gaagtatgtg tacgtatggc
     8401 tctccctcat cgtgctcacc tcactcaccg tctctgtggc aagactccac ctgacaagct
     8461 atgccattct catcgcgatc ctcatcgcca ccacaaaggc aggtatcgtg gtcacgttct
     8521 tcatgcacct caagaatgaa ccccttatcc tgaggctcat gctctttgtg gcgctcctgg
     8581 cattcaccct catcgccctc ctcacctttt cggacgtgtg gtacaggtga ggtgaccatg
     8641 tttattcccg gggcatcaaa ttcagcgcgc cacgtggacg caacgtttgc cgtcatcctc
     8701 gccattgagg tcttcctcct cttcgcggtg actgctgcca tgattttttt cgtcatcagg
     8761 taccggaaaa agaaaaatcc ccgttctgag aacgtcgagg gaggaatgct cctggagatc
     8821 gcatggaccg ccattcccac ggtcctggtc ctcatcatgt tctacatcgg gctgaaaagc
     8881 ttcatcctga tccggaaggt ccctgaaggg gccatgccgg tccaggtgac cgcccgccag
     8941 tggtcatggc aattcaccta cgcaaacgga aaacagagca gcacgctcgc agtacccctc
     9001 aacaggccgg tgaagctcat actccgctcc caggacgtgc tccactgcct ctacatccct
     9061 gccttcagga taaaggagga ttgtgtccct ggcttggaga cctatctctg gttcactgca
     9121 aaagagacgg gatcgtacga tatcttctgc accgaatact gcggtctcgg acattcggac
     9181 atgctctcaa aggtcgtggt tacgtctgca aaggaattcg atgcatggta tgcgggtgaa
     9241 ggggagaagg gcggtctggg tctcaccgtc ctggagtcca ggggatgtct cggctgtcat
     9301 tccatggacg gctcgaagaa gatagggcca accttcaagg gactctacgg ctcacaggta
     9361 acagtgctga ccagcggaaa ggagaggacc gtcacttcgg atgaagaata cctaaggaga
     9421 tccattctcg aaccaggtgc tgatattgta aagggatacc agaatatcat gcccgccctc
     9481 cccgtgacaa aggaagagct ggatgccatc atccagtatt tgaaaacgct gaaatgacgg
     9541 aagcggggca aaagaccatg ccactcctcg tgtatagtga gttatgcaaa gtgaggatat
     9601 ctcttttctc ggcattctct gctctcaccg ggcttctcct cgctgcgcgc gaattcaggc
     9661 cagaggcagt tgtgctcgtc gccggggtct ttctccttgc gtgcggctcc tctgccctca
     9721 accaatacca ggagaggaag acagatgtcc tcatgccaag gacagaggga agaccgattc
     9781 cctcagggag gatggaaccc tcccatgccc tgtttttctc cgttatgctt atttccctcg
     9841 gctccctcac actcctcctc accggcaggg cagttgctcc ggtccttggg cttctcgcag
     9901 tcatctggta taacgggctg tacacctatc tcaagagaaa aagcgccttt gcattgatcc
     9961 cgggggcact tgtgggcgcc ataccccctg ccataggctg ggtcaccgga gaggggactt
    10021 tttcggatcc cgggctcatt gccctctgct ttttcttctt tctgtggcag gtcccccact
    10081 tctggctcct catcctgagc tatggggagg aatacaggaa tgcaggtctc ccctctttga
    10141 ccgccatttt taccaggact caattgttgc gaataatcgt gaactggatt tttgccactg
    10201 cagtgagctg tctctttttg tctataagcg gcatggtact cactcccctg ataaactata
    10261 tgttgctcgg ggtctcctta tggctcatgg ccaccgccat aagactcctg attcaagggg
    10321 gagaagtcac ctcgacctat acctccacat tcaggaggat caacacctac atgttccttg
    10381 tgatgacact tttgtccgtc gacaaattga ttgtctaagc ctcgcccggg acctgatcga
    10441 gcgcccctgc ggtcaccctc ttgttttgat tgctgcggat catttcagat acaatttctc
    10501 ctgtccctaa gatcctcaat ggataacttc aacgtgaaat gcgaacagcg gatagcatgc
    10561 agaaggtcgc gccttttttg gaaggtttga gttcgtctgt tacagccact aacaaacgtt
    10621 tcagcgatca gagaacctat aacatctgct cacag
//
LOCUS       DATKOU010000135         9247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2486283, whole genome shotgun sequence.
ACCESSION   DATKOU010000135 DATKOU010000000
VERSION     DATKOU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486283"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VMU21_06190"
     CDS             complement(<1..283)
                     /locus_tag="VMU21_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HUO77152.1"
                     /translation="MMSYGLDINSISGLSERDAAKRLAEEGYNELPGTTERSILSIAL
                     EVIREPMFLLLIAGGAIYLFLGDIREAVMLLGFVLVVMGITLYQERKTER"
     gene            516..857
                     /locus_tag="VMU21_06195"
     CDS             516..857
                     /locus_tag="VMU21_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HUO77153.1"
                     /translation="MCEDNKTIAGAFLVGGLIGAAIALLYAPKAGRETRRDISKAAKK
                     IKRDAVDLVEDTIQSIDEFAGEVKDKATDIIERGVELSETAKKEVVKSFEYGQKIIEK
                     QKKRIIEGLGL"
     gene            877..3240
                     /locus_tag="VMU21_06200"
     CDS             877..3240
                     /locus_tag="VMU21_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HUO77154.1"
                     /translation="MITENALNLLEFHKLLKIISKYSHSEASEKAVGEITPLAHREEI
                     EKRLGQVHEIQRMSQENDPLRLSEFSDIAPLLTSLRPEGAVLDAKELSLFTPFLTVLS
                     DISSQMEERPDLPLLKEVSGQLTGFPQILILLKRSIDSEGNILDTASSLLSELRAQVR
                     QLEGRVRKRLEEMMRDERISLFLQDTFITERSGRWVIPVRMDSKGQVPGVVHDVSRSG
                     ETAFIEPLAIIGLSNELENLIAEQKAEEMRILRDICSRIRGVADEMGVQYGVIVHLDL
                     LYCISQFADELEMQIPRIDDAGMLHLVGARHPLLTLALEKTGAQGVVPLDVRLGGENT
                     VMVITGSNAGGKTIAIKTIGLLQLMALSGMPVPADSSSRFPLIHNLLIDIGDEQSIEN
                     SLSTFSAHVSNISGILKKANAHSLALIDELGTGTDPEEGAALACAVLKEIRSSGALLF
                     ATTHLTDIKGFVHRMEGMVNASMEFDQKSLTPLYKLREGEPGQSHALEIAKRYGLPDR
                     IIESAKGMLGGIKVEFDNLIADLNEKRLRYERGLRELDSQQKEMEEKNKLLQQTLFDA
                     ERQKKDLLAKAYREASELIADTKRQMRAFLDEVRKREKTKRREVIAQVETKQEEIVQK
                     LREYDMTDYGSPSLEEIREGDVVFVRSLGYDASVLEVDLRHKRLRVRSGGLEIEVPLS
                     DIGFKTGKSAEVKQEAVHIDHPDEIASSRMNLVGSRVDEALSRLEPFLNHASLAGLSE
                     VTIIHGFGTGILARAVREHLTGHPLVKNFRSGEQSEGGAGVTVATLV"
     gene            complement(3280..4797)
                     /locus_tag="VMU21_06205"
     CDS             complement(3280..4797)
                     /locus_tag="VMU21_06205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUO77155.1"
                     /translation="MDDRDRTKEELIDELKDLRQRIVQLEGTKNDLMRTEERLSKANR
                     TLRALSRCNEVLVHSSNEAELLDEICKIIIEEGGYRLCWVGYREYDSFKTVKSVAQAG
                     YEVGYFKKVKITWDDSELGRGPTGTAIRSGEPYICQNIPSDPRFAPWSEDAGKRGYSS
                     AIGLPLRGNSTTLGAITIYAAEADAFDEEEVKLLTELADNLAFGIQALRAREERMCSE
                     DALKSSEEKYRSLVESTEDSIYLVDREYRYLFMNKRHLERMNVLKEVALGQPYQKFHS
                     PEQNKKMVELVNRVFETGSSIQQEHLNRVDGRYFLRTLSPVTDKDGKIVAVTVVSKDI
                     TGIKQLEERLRALTLTDELTGLYNRRGFLALVEPLLKLARRQKKALYMLYADMDRLKE
                     INDTFGHKEGDLAIRDTANIFRSTYRESDIIARIGGDEFVVIPVEGANNDSSGIETRL
                     QEKIENHNTKGERPYKLSISCGIAHYDPGNATSIDELLLQGERLMYEQKNKKREG"
     gene            complement(4934..6115)
                     /locus_tag="VMU21_06210"
     CDS             complement(4934..6115)
                     /locus_tag="VMU21_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HUO77156.1"
                     /translation="MSETVSVKNIGLRGVKVADTKISFIDGEQGILIYRGFRIEEMAR
                     ESSFVETAYLLLHGTLPGGKELDSFSRQIMEARKLPGFIYESFKKWPKQSQPMDVLQA
                     AIPLLAMADPDLLEETREANVRKAIRLIARLPQVIAGWHRIRNGLEPLPVDDHLPHAA
                     HFLWQLNGTKPDEEMVHDLDTCLILHADHTFNASTFACREVVSTRAHMYAGVAAGAGA
                     LSGSLHGGANAEVMKMLRELESERDIPGWVKTQLDQGRRIMGMGHAVYKTTDPRAAFL
                     KEMSKRLGKKLGEEKWSRLSAEIEEASVKEFEKRGKPTIKPNVDFYSAPVYHMMGIPS
                     DLFTPIFAMSRIAGWCAHIIEEKFAEAQEKPMLYRPSTEYVGNYCGLMGCTYEPVTER
                     K"
     gene            complement(6112..8052)
                     /locus_tag="VMU21_06215"
     CDS             complement(6112..8052)
                     /locus_tag="VMU21_06215"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HUO77157.1"
                     /translation="MPKNLTYKILESHLVEGLLVAGTEIGIRIDHTLLQDATGTMALL
                     EFETLGIDKVKTELAAQYVDHNLLQTDSKNADDHRFLQTASARYGIIFSRPGNGVSHQ
                     VHMERFGVPGKTLLGADSHTPGAAGVSMLGIGAGGLDVALAMAGFPYYLPCPRVLGVR
                     LTGRFPDWVSAKDVILEMLGRYDVKGCVGKVIEYYGPGVKNLSATDRETIGNMGTELG
                     ATSTLFPSDENTKTYLEAQGRGNIWRELAPDEGAEYDEHDEIDLSSLEPLIACPSSPG
                     NVARVREVAGIRVDQVIVGSSVNSSFRDLMVVARIMEGRRCDPGTEFNVNPGSRQVLE
                     NVAHHGGVISLLGAGARIHEPGCLGCIGMGQAPGTGQVSLRTMPRNFPGRSGTKDDKI
                     YLCSPETIAAAALKGVITDPRDLSKEMDYPRIKEPDKYLTDESSLIFPSDELRNTVVV
                     RGPNIKPLPEFGPLPDTLEAEVVLRVGDNISTDTIMPAGSKILPLRSNIEAISEYIYS
                     QIAPDFAEECRKKGTVVVIGGENYGQGSSREHAALAPRFLGVRAKIVKSFARIHKANL
                     CNFGILPLIFKEPNDYDYFRKGSRVKFPDVRECVERGDREVLVEIEGKSVITILDVSD
                     RQRKHLLAGGTLNFVRKELQKK"
     gene            complement(8056..8550)
                     /locus_tag="VMU21_06220"
     CDS             complement(8056..8550)
                     /locus_tag="VMU21_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUO77158.1"
                     /translation="MTKKESKQSKELVRIEPGKTFSPFEEMEHRIGELFRRPFSLGPS
                     WLPRLRMPEMEELSPIVDIFEEGDDVVVKAELPGMKKEDIDVKLTENSVTISGEKKKE
                     EKVEKKNYYRMERSYGSFTRVFSLPAEVQTDKAKAQFTGGILQIRVPKTEEAKKKEKK
                     VPIE"
     gene            8762..>9247
                     /locus_tag="VMU21_06225"
     CDS             8762..>9247
                     /locus_tag="VMU21_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77159.1"
                     /translation="MNRLSWQAALGIALIALSAVLYFIEIEVFHSSRDTFFYFFQDIA
                     FVPIQVLLVTLIINRLLTVREKRSRLEKLNMVIGAFFSEVGTKLLTYFSDCDPNLKNT
                     RKDLIVTNNWSHKEFVRMKGRMKHYDYGVEIQKVDLVYLRTFFLEKREFLLRLLENPN
                     LL"
BASE COUNT         2170 a         2519 c         2252 g         2306 t
ORIGIN      
        1 ccctctcggt cttgcgctcc tgatagagcg tgatgcccat gactacaagc acaaacccga
       61 ggagcatcac cgcttcccgg atgtcgccta aaaaaagata gatcgcaccg ccggcaatca
      121 gaagaagaaa catgggctca cggatcactt caagcgcaat gctaagaata ctccgttccg
      181 tggtgccggg gagttcgtta tacccttctt ccgcaagcct ttttgcggca tccctttcgg
      241 aaaggccgct gatgctgttg atgtctaatc catagctcat cataaactgt ctatgttata
      301 ccctatatgg cgatgttaat caatgaagag attttgaata attttgccca ccatttgcca
      361 gctgagtgcg taacgattct ttgcttcagg aaagatgccc ttcggcagac atacaccatt
      421 ccgaagacct gtggcgcatt tgactcttca gaccattcct gctagagtta tattattaag
      481 ctatcataga gagctttttc gaaaggagaa ctaccatgtg cgaagacaat aagaccattg
      541 ccggggcgtt cctggtgggt gggctgatcg gagcagctat cgcccttctc tacgcgccca
      601 aagcaggccg cgaaacgagg agggacatct ccaaggcagc aaaaaagata aagcgtgacg
      661 ccgttgacct ggttgaggat accatccaga gcattgatga gttcgcaggc gaggtgaagg
      721 ataaggccac agacattatc gaaaggggcg tggagctgtc tgaaactgcc aaaaaagaag
      781 tggtgaagtc ctttgaatac ggacagaaga ttatagagaa acagaagaaa aggattattg
      841 agggacttgg gctttaaggc tttttcagac tgatgaatga taacagagaa cgccctcaat
      901 ctcctcgagt ttcacaaact cctcaagatc atctcgaagt attcacatag cgaagcctcg
      961 gaaaaggccg taggagaaat cactcccctc gcgcataggg aggagattga aaaaaggctg
     1021 ggacaggtcc atgagataca aaggatgtcc caggagaatg atcccctcag gctctcggag
     1081 ttttccgata ttgcgcccct ccttaccagt ctgaggcccg agggggctgt gctggacgcc
     1141 aaggagcttt ccctcttcac cccatttctg accgtccttt ccgacatatc ctcccaaatg
     1201 gaagaaagac ctgacctccc tctattgaaa gaggtctctg gccagctcac cggatttccc
     1261 cagatcctca tattgctcaa gaggtccatt gacagcgagg gaaatatcct tgataccgct
     1321 tcatctctcc tctccgaact gagggctcag gtcaggcagc ttgaagggcg ggtgagaaag
     1381 agactggagg agatgatgag ggatgagagg atctctctct ttctccaaga taccttcatc
     1441 accgagaggt cagggagatg ggtgataccg gtgaggatgg attccaaggg gcaggtgccc
     1501 ggggttgtcc atgacgtctc acgatccggc gagaccgcat tcatagagcc cctggccatc
     1561 atcggcctct ccaatgagct cgaaaacctc atcgcagagc agaaggcaga ggagatgcgc
     1621 atcctcaggg acatctgctc caggataagg ggcgtagctg atgagatggg cgtccagtat
     1681 ggggtcattg tccaccttga cctgctgtac tgcatttccc aatttgccga tgaactggag
     1741 atgcagattc cccggataga tgacgcgggc atgcttcatc ttgtgggagc aaggcatccc
     1801 ctcctcaccc tcgcactgga gaagacagga gcacaggggg tcgttcccct tgatgtccgc
     1861 cttggaggag aaaacacggt gatggtgatc accggctcca atgcaggggg caagaccatt
     1921 gccattaaga ccatcggcct gcttcagctt atggccctct ctggaatgcc ggtccccgca
     1981 gactcttcat cccgttttcc cctcatccac aacctcctca tcgatatagg tgacgaacaa
     2041 tccatagaga acagcctctc cacattttcc gcccatgtgt ccaacatctc cgggatactg
     2101 aaaaaagcta atgcgcactc ccttgcgctc attgatgagc tggggaccgg caccgaccct
     2161 gaggaaggag ctgcgctcgc atgtgcggtg ctgaaggaga tacggagcag tggcgccctc
     2221 ctttttgcca caacgcacct cactgacatc aagggctttg tgcacaggat ggagggaatg
     2281 gtgaatgcct caatggaatt tgaccagaaa tccctcaccc cgttatacaa attacgggaa
     2341 ggcgaacccg gccagtccca tgcactggag atcgcaaaga gatacgggct gcctgatcgc
     2401 attatcgaat cagcgaaagg gatgctgggt ggcatcaagg tggagttcga caacctcatc
     2461 gctgatctca atgaaaagag gctcaggtat gaaagaggtc tgagggagct tgacagtcag
     2521 cagaaggaga tggaagagaa aaacaagctc ctgcaacaga ccctctttga tgctgaacgc
     2581 cagaaaaaag acctccttgc aaaggcgtac cgggaagcct ctgagctcat tgcagataca
     2641 aaaaggcaga tgcgagcctt ccttgatgag gtaaggaaaa gggaaaagac aaagaggcgt
     2701 gaggtcatcg cacaggtcga gacaaagcag gaagagattg tgcagaaact cagagaatac
     2761 gacatgaccg attacgggtc cccttccctt gaggagatca gggaaggaga tgtggtcttt
     2821 gtgaggtcac tgggatatga cgcctctgtg ctggaggtgg acctgaggca caagcggctg
     2881 agggtgagga gcggcggcct ggagatagag gtccctttgt ctgacatagg atttaagaca
     2941 ggaaaatcag cagaggtgaa acaggaggcc gttcatatag accatccaga cgagatagct
     3001 tcatccagga tgaaccttgt agggtcaagg gttgatgagg cgctgtcgag actcgagccc
     3061 tttctgaatc atgcctcttt ggcaggacta tcagaagtga ccatcatcca tggcttcggc
     3121 accggcatac ttgccagagc cgtgcgtgag cacctcaccg gccatccgct tgtaaaaaat
     3181 ttcaggagcg gggagcaatc cgagggaggg gctggtgtga ccgtggcgac gcttgtctag
     3241 tttaagaaca acgtgatcga atagaacggg tgataatccc tacccttccc gtttcttatt
     3301 tttctgctca tacatcaacc gttctccctg aaggagaagt tcatcaatag aggtagcatt
     3361 ccctggatca tagtgcgcta ttccgcagct tatggacagt ttgtacggcc tctccccttt
     3421 cgtattgtga ttctcgatct tttcctgcag acgggtttcg attcccgaac tatcgttgtt
     3481 cgcaccctcc acaggaatga ccacaaattc atctccgcca atgcgcgcaa tgatatcgga
     3541 ttccctgtac gtggacctga aaatattggc cgtatccctt attgcaagat caccctcctt
     3601 gtgcccgaat gtgtcattga tctccttgag cctgtccata tccgcgtaga gcatgtaaag
     3661 tgccttcttc tgccgtcgcg ccagctttaa caaaggctcg accagggcca agaaacctcg
     3721 ccggttgtag aggccggtga gctcatcggt gagggtcagc gcacgtaatc tctcctccag
     3781 ctgtttgatc ccggtaatgt cctttgaaac aaccgtgacc gccacgattt tcccgtcttt
     3841 atctgttaca gggctgagtg tacggagaaa gtacctgccg tccacgcgat tcagatgctc
     3901 ttgctgtatc gagctacctg tttcgaatac cctattcaca agctcgacca ttttcttgtt
     3961 ctgttcagga gaatggaact tttggtaagg ttgtcccagt gccacctcct ttaaaacatt
     4021 catccgttcc agatgccttt tattcatgaa caggtatcgg tactccctgt caaccagata
     4081 gatggaatcc tcagtggatt ccactaaaga acgatatttt tcttcgctcg atttcaaggc
     4141 gtcctcagag cacattcgtt cctcacgggc gcggagcgcc tggattccga acgcaaggtt
     4201 atctgccagt tcggtcagga gcttcacttc ctcctcatca aatgcatcag cttccgctgc
     4261 atatatggta atggcgccta gggttgtgga atttcctctg aggggtaagc caattgcaga
     4321 ggagtacccc cgtttcccgg catcttcact ccagggggca aaacggggat cgctagggat
     4381 gttctggcag atatacggct caccactcct tattgcggtg ccggtgggtc cccgccccag
     4441 ttcagagtcg tcccatgtga tcttcacctt cttaaaatac cccacctcgt atcctgcctg
     4501 tgctacagac tttacggtct tgaatgaatc atactcgcgg tagccgaccc agcagaggcg
     4561 gtatcccccc tcttctatga taatcttgca tatttcatcc aagagttctg cctcattgga
     4621 ggagtgcacc agtacctcat tgcaccggct gagagccctg agcgtccgat tggccttgct
     4681 caatctctcc tcagtcctca tgagatcatt cttcgtgcct tccagctgaa cgatccgctg
     4741 gcgcaggtcc ttcagttcat caatcagctc ttcttttgtc ctgtcccggt catccatagc
     4801 aatttccgct gttcagttta ttactaagtc aaagaatatt caagaaaaaa acattgtgtc
     4861 ttaatgccgg aaagataatc tgttcctcca atgcactacg acctcctctt atccaaacag
     4921 ggggtgccgc ccgctatttt ctctctgtaa caggttcata ggtgcagccc ataagtccgc
     4981 agtaattgcc gacatattcg gttgagggtc tgtagagcat gggcttttcc tgggcctctg
     5041 caaatttctc ttctatgata tgggcgcacc atcccgcaat cctcgacatg gcaaaaatgg
     5101 gagtaaacag gtcactcggt attcccatca tatggtagac cggggcgctg tagaaatcca
     5161 cattcggttt aatggtcggc ttccccctct tttcgaattc cttcaccgat gcctcttcta
     5221 tctctgctga taatcgtgac catttctcct ccccaagctt cttgcccagc cgttttgaca
     5281 tctctttcag aaacgctgcc ctgggatctg tggttttgta taccgcatgg cccatcccca
     5341 ttatcctcct gccctgatcc agctgcgtct tcacccagcc gggtatatcc ctttcggatt
     5401 ccagttctct gagcattttc atcacctcgg cgttggcgcc gccatgcaaa ctcccagaaa
     5461 gggctccagc acccgcggca acccctgcat acatatgcgc cctggtggat actacctcgc
     5521 gacaggcaaa ggtggaggca ttaaatgtgt ggtctgcatg gaggatgagg caggtatcaa
     5581 ggtcatgcac catttcttcg tcaggctttg ttccattgag ctgccacagg aaatgggcgg
     5641 catgtggcag atggtcatcc acggggagcg gttcgagacc atttcgtatc ctgtgccagc
     5701 cggcaatcac ctggggcaga cgggctatga gcctgatggc cttcctcaca ttcgcttccc
     5761 ttgtctcttc caaaaggtct gggtctgcca tggcgagaag gggtatggcg gcctggagca
     5821 cgtccatggg ttgagactgt ttcggccatt ttttgaaact ctcatagatg aacccgggga
     5881 gcttcctcgc ttccataatt tgtctgctga aggaatccag ttcctttccg cctggcaatg
     5941 tcccgtgcaa aaggagatag gcggtctcca cgaatgaaga ttccctagcc atctcctcaa
     6001 tgcgaaaacc gcgataaatc agtatgccct gctctccgtc gataaagctg atctttgtgt
     6061 ccgccacttt tactcccctt aggccgatat tttttaccga gaccgtttcg ctcatttttt
     6121 ttgcagttcc tttctcacaa aattgagagt cccccccgca aggaggtgct tcctctggcg
     6181 gtccgagaca tccagaatag tgatgaccga ctttccctca atctccacaa ggacctcccg
     6241 gtctccccgt tctacgcatt ctcgcacatc aggaaatttt accctggacc ctttgcggaa
     6301 ataatcatag tcattcggct ccttaaatat gaggggaagg atgccgaagt tacagagatt
     6361 ggctttgtga attcgtgcaa aactcttcac gattttggcc cggacaccca ggaagcgggg
     6421 agcaagggca gcgtgctccc ggctcgatcc ctggccgtag ttttcacccc ctatgaccac
     6481 cacagtgccc tttttcctgc actcctctgc aaaatcaggg gcgatttgcg aatagatata
     6541 ttcacttatg gcctcgatat tggagcgcag gggcaggatc ttactccctg cgggcatgat
     6601 ggtatcggtg gagatattgt ctcccactct gagcaccacc tctgcctcca gtgtgtcggg
     6661 gagtggccca aattcaggga ggggcttgat attcggtccc cttaccacaa cagtgttccg
     6721 cagctcatct gaggggaata tgagggaaga ttcgtctgtg aggtatttgt cgggttcctt
     6781 aatacgggga taatccatct cctttgacag gtccctcgga tcagtgatga cccccttaag
     6841 ggccgcggca gcaatggttt cgggagaaca gagatatatc ttatcatcct ttgtgcccga
     6901 acgtcccggg aaattccgtg gcatggtacg aagactcacc tgccctgtac ccggggcctg
     6961 ccccataccg atgcacccaa ggcagccagg ctcgtgtatc cgcgcacctg ctccaaggag
     7021 ggatatcacc cccccgtgat gtgccacatt ttcaagaacc tgcctgcttc ccggatttac
     7081 attaaattcc gtcccagggt cgcagcgccg tccttccatg attcttgcca ccaccataag
     7141 gtcacggaaa gaggaattga cactgctccc cacgataacc tgatccaccc tgatccccgc
     7201 cacttcccta accctcgcca cattcccggg tgaggaaggg catgcaataa ggggttcgag
     7261 ggatgacagg tcgatctcgt cgtgctcgtc atactcagca ccctcatcag gagccagttc
     7321 tctccagata ttcccgcgcc cctgggcctc aagataggtt ttggtattct catccgaggg
     7381 gaatagggta ctggtagcac ccagctctgt tcccatattg ccgatggtct ccctgtctgt
     7441 ggcagagaga tttttcactc cgggcccgta atactcgatg acctttccca cgcacccttt
     7501 gacgtcatac cgccccagca tctcaaggat aacgtctttt gcgctcaccc agtccggaaa
     7561 tcttcctgtg agtcttacac ccaggaccct aggacaggga agataataag gaaagcctgc
     7621 catggccagc gccacgtcca gtcctcctgc gccaatgcca agcatcgaaa cgcctgctgc
     7681 gccgggagta tggctgtctg ctccaagaag ggttttgccc gggacaccga aacgctccat
     7741 atgtacctga tgggataccc cgttgcccgg acggctgaaa atgattccgt agcgtgcaga
     7801 ggcagtctgg aggaaccggt ggtcatcggc attcttgcta tcagtctgga gaaggttgtg
     7861 gtccacatac tgtgcggcaa gctctgtttt cactttgtcg attcccaggg tctcgaattc
     7921 caggagggcc atagtccccg tggcatcctg aagcagggtg tgatcaatcc tgatcccaat
     7981 ctcggtgcct gcgacaagca ggccctccac gagatgggac tccaaaatct tataggtgag
     8041 attcttcggc ataccttatt ctataggtac ctttttttcc ttctttttcg cctcctctgt
     8101 cttggggacc ctaatctgga gtatcccccc tgtaaactgt gcctttgcct tatctgtctg
     8161 gacctcagca gggagactga acacccgggt aaaggagccg taggagcgct ccatccgata
     8221 gtagttcttt ttttcgacct tttcctcctt cttcttttcc cctgaaatgg tgacactatt
     8281 ttcggtgagc tttacatcaa tatcctcttt cttcatcccg ggcagctctg ccttcaccac
     8341 cacatcgtct ccctcctcaa agatatccac tatgggagaa agctcttcca tctcaggcat
     8401 cctcaacctt ggaagccatg aaggaccaag ggaaaagggc ctcctgaaca attccccgat
     8461 ccgatgctcc atctcctcaa agggagaaaa ggtcttcccc ggttcaatcc tgaccagttc
     8521 ctttgactgc tttgattcct tcttcgtcat gccacacctc cgtaagtaaa gacaccttca
     8581 ttcaaacgta tcccatatag ttattctatt tcgtctcagg aaaatgtcaa tcgcccaatc
     8641 acacttccag gaaagagaat cgtttcttag gaaaagacat gggacaataa atcttttaaa
     8701 agccacacac atgaaataat gtgtgcttgc gatgcgccca ttcagtttga tacactctat
     8761 catgaaccgt ctcagctggc aggcagcact tgggattgcg ttgattgcct tatccgcagt
     8821 tctctatttt atagaaattg aggtctttca ttcctcccgg gatactttct tttatttttt
     8881 tcaggatatc gcctttgtcc ctattcaggt ccttctcgtt accctgatca taaaccgttt
     8941 gctgacagtg agggaaaaac gctcccgcct ggaaaaactg aacatggtca taggcgcctt
     9001 cttcagtgaa gtggggacta agctccttac ctatttttcg gattgcgacc cgaaccttaa
     9061 gaacaccaga aaggatctca tcgtcaccaa caattggtct cataaagaat ttgtaaggat
     9121 gaaaggccgt atgaagcatt acgactatgg ggtcgagatt cagaaggtcg accttgtgta
     9181 cctgcgcact tttttccttg aaaaaaggga attcctcctg aggctccttg aaaatccaaa
     9241 ccttctc
//
LOCUS       DATKOU010000136         4378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5567850, whole genome shotgun sequence.
ACCESSION   DATKOU010000136 DATKOU010000000
VERSION     DATKOU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5567850"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="rpsD"
                     /locus_tag="VMU21_06230"
     CDS             <1..431
                     /gene="rpsD"
                     /locus_tag="VMU21_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HUO77160.1"
                     /translation="YGLVETQFRRYFHEAERRKGITGEILLQLLECRLDNVVYRMGFS
                     ANRRQARQLITHGHFKVNGKSVSIPSYSTRAGDVVEVRESSRDIESIQENLDKAAHRG
                     LASWLEIDAANFKGKIVHVPSREEIQLPVQEQLIVELYSK"
     gene            467..1459
                     /locus_tag="VMU21_06235"
     CDS             467..1459
                     /locus_tag="VMU21_06235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HUO77161.1"
                     /translation="MDLRKKGFQLPDKIRFDEETLTDTYGKLIAEPFERGYGPTLGNS
                     LRRVLISSLEGAAVIAVKIRGALHEFSHITGVKEDVMDIILNIKKLRFKMYSDNKKIA
                     TIKVSGPKEAKGGDLHVDSAIEVLNPEQVIATLDKGVSFEAEMYIRKGKGYVASELNK
                     EADLPVDMLAIDSVFSPVKKVNFSVEKARVGRATDYDRLVMEIWTDGSITPQHAVSQA
                     ASIIIDYMDLFISEEEGTGAEYEESTAMASVSGSASFNENLLKNVDELELSVRASNCL
                     KNANIKTISELVQKSEYEMLKTKNFGRKSLNEIKEILHSMGLHLGMRIDTDAVK"
     gene            1480..1920
                     /gene="rplQ"
                     /locus_tag="VMU21_06240"
     CDS             1480..1920
                     /gene="rplQ"
                     /locus_tag="VMU21_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HUO77162.1"
                     /translation="MRHRVSGKLFGRTANQRKALLRGLMASLFEHQRIETTLAKAKEV
                     KKLAERIITLGIKGDLHSKRVALSHVPSRSSIAKLFNEIAPRFSGRNGGYLRLVQTRN
                     RVNDAAPMAVLEFVDYEEVRKATEGKKAETKKTKEKKVEPKGNE"
     gene            complement(2193..2726)
                     /locus_tag="VMU21_06245"
     CDS             complement(2193..2726)
                     /locus_tag="VMU21_06245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77163.1"
                     /translation="MKKIVAILLLAFVVFACKQKEGAKPQASLPDGPIQSLPGKTLEE
                     EKLLRDVLSKDPKNLNAWLQLGNIMMDSARFNEAVDAYAKALELDPKNVDVRVDMGTC
                     LRNSGKPDLAIKEYKKAIEINPNHVYAHINMGVVLSDKKEYKAAVSELEKGLSLNPNL
                     PNAQAIRQEIERMKTLK"
     gene            complement(2816..3658)
                     /locus_tag="VMU21_06250"
     CDS             complement(2816..3658)
                     /locus_tag="VMU21_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77164.1"
                     /translation="MGPAEFCPVGKLEKLLVATDRSEFSEGAVREAITFAKKCSSRLF
                     AISVLESNPEYETIGSNVFQKEEAEALAYLESVKERASREGLGCEIILRQADDPSQTI
                     VSEASKKNVDMIVIGRHGRTGLMKVLMGQVAAKVIGNASCKVLVVPRAAKIEYRNILV
                     ATDGSVHANAAVTEAIAIAKRCGSSLIALSAMHDEGERAEATAHANMAVELAQKEGVS
                     AEAVTPMGGSFTAIVETACGRGVDLIVMGTYGKTGLKKFLMGSSTEKVIGNAGCAVLV
                     VKSL"
     gene            complement(3730..>4378)
                     /locus_tag="VMU21_06255"
     CDS             complement(3730..>4378)
                     /locus_tag="VMU21_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HUO77165.1"
                     /translation="VALESKKFDFTSETPRAESKAFSALVGDWRIDKDGSNLVYAVDG
                     KERVPGLSAGADGYARDLYGEKSAEFLKGIEALKDYPLTVCKEFQNFKNGTLTVSFKA
                     ISGKGDQAAGIEFNIRQSGEYLAMRANALENNLGLFKFENGKRSPLQWAQDVPPPERE
                     WHTLKVVIKKKKIEGYLDDKKYIDYTYDDTIDGRIGLWSKADSHVFFNNFTVQSE"
BASE COUNT         1127 a         1041 c         1070 g         1140 t
ORIGIN      
        1 catacgggct cgtcgaaacc caattcagaa gatatttcca cgaggcagaa cgtcgcaaag
       61 gcattaccgg tgaaatcctt ctccagctcc ttgaatgccg tcttgacaat gtagtgtaca
      121 ggatggggtt ttctgcaaac agaaggcagg cgcgacaatt gatcactcac ggacatttta
      181 aggtaaacgg gaagtctgtg agcataccct cctatagcac acgggcgggt gacgtggtcg
      241 aggtcaggga gtcgagcagg gatatagagt caattcaaga aaatctggac aaggccgcgc
      301 atcgcgggct tgccagctgg ctcgagatag atgcggctaa ctttaagggc aagattgtgc
      361 atgttccgtc cagggaggag atacagcttc ctgtgcagga acagcttatc gttgaattat
      421 attccaaata gaaccaggtt aaaatttaag gcataggagg ccttatatgg atttaagaaa
      481 aaaaggcttt caattacccg ataagattcg gtttgatgaa gagacattga ccgataccta
      541 cgggaaactc atcgcagaac cttttgaaag gggttatggc ccgacgttgg ggaattccct
      601 caggagggtc ttgatttcgt ctctggaagg tgctgcggtc attgcggtga agataagggg
      661 cgcactccac gagttttccc atatcacagg ggtgaaggaa gatgttatgg atattatcct
      721 taacataaag aagctgagat tcaagatgta ttcggacaac aagaagatcg ctaccattaa
      781 ggtgagcggt ccaaaagaag caaagggcgg ggacttgcat gtggactcag ctattgaggt
      841 gcttaatccc gaacaggtaa tcgccacgct ggataagggg gtatcctttg aggctgagat
      901 gtacatcaga aaaggaaagg gatatgtagc ctcggagttg aacaaggaag cggatctccc
      961 agtggatatg cttgccatag actcggtgtt cagtcctgtc aagaaggtga acttctctgt
     1021 tgagaaggca agggtgggaa gggccacgga ttatgacagg ctggtaatgg agatatggac
     1081 agacgggagc atcacgcccc aacacgcagt ttcacaggca gcatccataa tcatagatta
     1141 catggacctc tttatatccg aagaagaggg aacgggcgcg gagtacgaag agagtacggc
     1201 catggcatcc gtttcgggca gcgcctcctt taatgagaat ctcttgaaga atgtggatga
     1261 actcgagctc tcggtgaggg cttccaactg cctgaagaat gcgaatatca agaccatctc
     1321 cgaactggtg cagaagtcag agtacgagat gctaaagaca aagaattttg gtcgaaagtc
     1381 tttgaatgag ataaaggaga tactccattc catggggttg cacctcggta tgagaattga
     1441 cacggatgca gttaagtaat ggtaggggga tgaaagggta tgcgtcatag agtttccggg
     1501 aaactatttg gtcgaactgc gaatcagaga aaggcccttc tgagagggct tatggcatcg
     1561 ttattcgagc atcagaggat agagacaaca ctggccaagg ccaaggaagt gaaaaaactg
     1621 gcagaaagaa tcattaccct gggcataaaa ggagacctcc attcaaaacg ggtggccctt
     1681 tcccatgtgc ctagcaggag ttccattgcc aaactcttca atgagatcgc cccaagattt
     1741 tcggggagga acgggggata cctaaggctt gtccagacaa gaaacagagt gaacgatgct
     1801 gccccgatgg ctgtccttga gtttgtagat tatgaagagg taagaaaggc aacggaaggg
     1861 aaaaaagccg agactaagaa gacaaaggaa aagaaagtgg aacccaaggg caatgagtga
     1921 tttgctttta gtggttattg ttctcgtcgt gtggcttgtt ctccagctcg tggtgttccc
     1981 taagcttggg atctccacct gaggggtgaa tcccaagggg tcagggacct ccaaaaagaa
     2041 atgttgagat aaccaaatag cacaaaagaa tatctgggga cgcaggcttc agcctgcgtt
     2101 ttttgttatg atgggaacgc cctcccggca taaagattgt gtttacgacc tgtatctttt
     2161 ccttcttcat gcgttgggaa ataaatgaaa agttatttca gcgtcttcat cctctctatc
     2221 tcctgcctga tagcctgtgc gttgggtaaa ttcggattaa gactaagacc cttttcaagc
     2281 tcgctcactg cggccttgta ctccttctta tcagagagga caacccccat atttatatgg
     2341 gcatatacgt ggttcggatt tatttctatg gcctttttgt attccttgat ggcgagatcg
     2401 ggtttcccgg aatttctcaa gcatgtgccc atgtccacac gaacatccac attcttcggg
     2461 tcaagttcaa gggcctttgc gtacgcgtct acggcctcgt tgaatcttgc cgagtccatc
     2521 attatgtttc caagctgtag ccacgcattc agattcttcg ggtccttgga gagcacatct
     2581 ctaaggagtt tctcttcttc taatgtcttg ccaggcagag actggatcgg accatcgggg
     2641 agcgaagcct gcggctttgc tccttccttc tgtttgcatg cgaacactac aaaagccagt
     2701 aatagaatag ccactatttt cttcatctct tcacctctcc ataatttgcc ctattgtaac
     2761 aaaactttgg ggcaattgat aaggggcaaa agccggttac gtataatgag acatctcaca
     2821 gagatttcac cacgagcact gcgcagccgg cgtttccaat gaccttctcg gttgagcttc
     2881 ccatgaggaa cttcttcagt ccggtttttc cgtaggttcc cataactatc aggtcaacgc
     2941 ccctgccgca tgccgtctcg acgattgcag tgaacgaccc ccccataggg gtgaccgctt
     3001 cagcagaaac cccttccttc tgcgccagtt cgacagccat gttcgcgtgg gccgttgcct
     3061 ccgccctttc accttcgtcg tgcatggcgg agagagcgat aagactgctc ccacagcgtt
     3121 tcgcaatggc gatcgcttcc gttacggctg cattcgcatg gaccgaaccg tctgtggcga
     3181 cgaggatatt ccggtattcg atcttcgccg ccctcgggac aaccaaaacc ttgcaggagg
     3241 cattccctat aacttttgcg gcaacttgac ccatgagaac cttcatcagt cctgtgcggc
     3301 catgcctgcc gattactatc atgtccacgt tctttttgct tgcctcgctt actatcgtct
     3361 gtgaaggatc gtctgcttgg cggaggataa tctcgcaacc aaggccttct cgtgacgctc
     3421 tttccttaac agattcaaga taggccaatg cctccgcctc ctctttttga aaaacattag
     3481 aaccgatcgt ctcatactca ggattcgatt caagcaccga tatcgcgaaa agcctgctcg
     3541 agcacttctt tgcgaaggtt atcgcctccc ttacggctcc ttcactgaat tcagaacggt
     3601 ccgttgctac cagcagcttc tcaagttttc ccactggaca aaattctgca ggacccatga
     3661 taatttctcc ttccatcatc aatttgccta atccgaggca tcaggattaa ggttatgaga
     3721 taactccctt tactcagatt gtaccgtaaa attgttgaaa aagacatgcg aatcagcctt
     3781 tgaccagagt ccgattcttc cgtctatagt atcgtcataa gtataatcga tgtatttctt
     3841 gtcatcgaga tacccctcaa tcttcttttt cttgatcacc acttttagtg tgtgccattc
     3901 tctttcgggc ggagggacat cttgtgccca ttgcaaaggt gaacgtttcc cattttcgaa
     3961 cttaaaaagc ccgagattat tttccagggc attggccctc atagcaagat actcaccgct
     4021 ctgtctgata ttgaattcaa ttccggctgc ctggtctcct ttcccactga ttgctttaaa
     4081 ggatactgtc agcgttccat ttttgaagtt ttgaaattcc ttgcacacag tgagaggata
     4141 atctttcaat gcttcgatgc ctttgagaaa ttcggcggac ttctcgccgt acagatctct
     4201 tgcatagcca tcagcgcctg ctgataaccc cggcactctt tctttgccat cgacggcata
     4261 tacaaggtta gagccatcct tatcgatgcg ccagtcgcca accagagcgc taaatgcctt
     4321 tgattcagcc ctaggtgttt cactagtaaa gtcaaacttc ttactctcaa gtgcaact
//
LOCUS       DATKOU010000137         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2872678, whole genome shotgun sequence.
ACCESSION   DATKOU010000137 DATKOU010000000
VERSION     DATKOU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872678"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VMU21_06260"
     CDS             <1..249
                     /locus_tag="VMU21_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77166.1"
                     /translation="DSISLTADSLLKDIGSLFNDKNKKELGNILQNTNSAIVSGSTNI
                     DNVLQSLNRTAEELRDVLQEVKNKPWTVVYKEERRREE"
     gene            251..838
                     /locus_tag="VMU21_06265"
     CDS             251..838
                     /locus_tag="VMU21_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HUO77167.1"
                     /translation="MLKRMTMFLLIAVFTVCAACSMPETQIYSLHLPTPKNSPNKNTN
                     ASIAILIHSPKYLAQPYIAYRKSPYELLISRYSKWESSPEEIVSHAMKDALSATGFFK
                     EIRTSYVVPAGFYSLTIQLKRFERIDEGDDSFGELSFEANLSSPNGKDVYQGSVSKRI
                     KLEGRSFASLAKGLSTSLAEGITEISGNVKRSLRQ"
     gene            complement(1003..2166)
                     /locus_tag="VMU21_06270"
     CDS             complement(1003..2166)
                     /locus_tag="VMU21_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUO77168.1"
                     /translation="MKEILGFPRNVFVLGIVSLFMDVSSEMIYPLIPLYLSNVLHASK
                     TSIGVIEGIAESTASILKVFSGWLSDRVGKRKALIFWGYGISVFSRPILATAGSWVHV
                     LIYRFTDRFGKGVRTAPRDAIIADSTGREILGKAFGFHRSMDTVGAVIGPGFAFVLMA
                     IFHDSIQTVFWLSIIPGLFAVLTIAFFVKDVKGSRGDEPPRISMRGFDRRFKLFLVIV
                     AIFTLGKTSDAFLVLRAQDLGVKAGTIPLLYLSFNAVSAALSTPAGILADRVGKRKVI
                     LASYVLFSLVFVGFALSTSPLHAWLLFGAYGLFVAMNEGVQRAYVATIIKPEIKATSY
                     GVYHTVIGLAALPSGIVGGSLWQHIAPQALFYYGAAMSFLSCILFALFLFEKR"
     gene            2185..3219
                     /gene="rtcA"
                     /locus_tag="VMU21_06275"
     CDS             2185..3219
                     /gene="rtcA"
                     /locus_tag="VMU21_06275"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HUO77169.1"
                     /translation="MIEIDGSFGEGGGQILRTALSFSCLFHKPFRIFNIRRSRKKPGL
                     MPQHLTGVRAAQLLSRAKVQGDRAGSVELSFSPGEVKGGSFFFDIGTAGSTMLVLQTL
                     IPALIFSGEKTILTLAGGTHVPFSPSFHYVRDVFLPFLKKAGIEIALSVESYGFYPKG
                     GGRIRVELFPTKEVMPLHITERGKMLRVKGISGVANLPSSIAERQRNAALGKLLPHNT
                     DCGYQTDIELLEVPSPGQGTFIFLKAESEHSIAGFTTLGERGKRAEAVGEEAATELLE
                     YLRTDAALGPHLADQIVPYLSLCKEQSIFTTCRITHHLLTNLRVIGLFHEFQYSIKGE
                     IGMPGTVRIN"
     gene            complement(3240..3833)
                     /locus_tag="VMU21_06280"
     CDS             complement(3240..3833)
                     /locus_tag="VMU21_06280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUO77170.1"
                     /translation="MKIVGISLAVMIILGAVVFGYAEVKEKKFTAEEIKKMAETKAVI
                     ETKLGTIELKFFPEVAPNHVNNFIELAKKGFYDGTTFHRVIPGFMIQGGDPNTKNPDK
                     SKHGMGGPGYTVKAEFNDKPHKRGTLSMARAANPDSAGSQFFICVADAPFLNKQYTVF
                     GEVVSGMDVADKIVSQPRDPRDNPNERVEMKVKIVEK"
     gene            3971..>4242
                     /locus_tag="VMU21_06285"
     CDS             3971..>4242
                     /locus_tag="VMU21_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77171.1"
                     /translation="MKKKNVCIGIACLFVFCASMVSGTSLDAKTPESVESQKKAVVAV
                     SVDDRYGRRIVSGSGFIVDREGVVATSCNLITKFLESQENTMAVELA"
BASE COUNT         1144 a         1115 c         1025 g          958 t
ORIGIN      
        1 gacagcattt cccttacagc ggacagcctc cttaaagata ttgggagcct cttcaacgac
       61 aagaacaaaa aggagctagg aaatatcctc cagaatacga attctgccat cgtctcgggt
      121 tcaacaaata tcgataatgt cctccaaagc ctcaacagga cagccgaaga actcagggac
      181 gtcctccagg aggtaaaaaa caagccatgg accgtagtct acaaggaaga aaggagaagg
      241 gaggagtgaa atgctgaagc gaatgaccat gttcttgctt atcgcggtgt tcacggtgtg
      301 tgcggcatgc agcatgcccg agacccagat ctacagtcta cacctgccta caccaaagaa
      361 ttcgccgaac aagaacacga atgcctccat tgccatactg atacattctc ccaaatacct
      421 cgcccagcct tacattgcct acaggaagtc cccttacgag cttctcatat caagatactc
      481 aaaatgggaa tcttctcctg aagagatagt gagccatgcc atgaaggatg ccctctccgc
      541 aaccggcttc tttaaagaga taaggacatc ctatgtggta ccagcaggtt tttactccct
      601 taccatacag ctcaagcggt tcgaaaggat agacgagggg gatgattcct tcggcgaact
      661 ctcctttgag gcaaacctca gttcgcctaa cggcaaagac gtctaccagg ggagcgtcag
      721 caaaaggata aaactggaag gccggagctt tgcgagtttg gcaaagggat tgagcaccag
      781 ccttgcagag ggaataacgg agatcagcgg gaacgtaaag agatccctca gacaataatc
      841 gccttttctg accggaccga caacctcaaa gggacgatgg gccgggcctc ttaaggaaaa
      901 catcaatctc cccattgcca tcctctcttc atttcaaaag ggttttctct cagctgtacc
      961 aagagatatt tactgattgc agcagcaaga gaacgctccc tttcatcgct tttcaaaaag
     1021 aaagagagcg aagagtatgc aggacaaaaa agacatggcc gcgccgtaat agaacaaggc
     1081 ctggggggca atatgctgcc atagggagcc tccgacaatc ccggaaggaa gggcagcgag
     1141 tccgatgacg gtatgataaa caccgtaact cgtcgccttt atctcgggct ttataatggt
     1201 cgccacatag gccctctgca ctccttcatt catggcgaca aacaacccat aggccccaaa
     1261 aagaagccag gcgtggagag ggctcgtaga caacgcaaaa cccacaaata cgagcgaaaa
     1321 gaggacatag ctcgcgagaa tgactttccg tttgcctacc ctgtcagcca gtattcctgc
     1381 aggagtcgag agggcagccg acaccgcatt aaaggagaga tagaggagag gtattgtccc
     1441 agccttgaca ccaagatcct gcgctctcaa cacaagaaat gcgtccgacg tcttacccag
     1501 ggtgaaaata gccacaatca ccagaaaaag tttaaacctt ctgtcaaatc ccctcatgct
     1561 gatacgggga ggctcgtctc ccctggagcc ttttacgtcc ttcacaaaaa aggcgatggt
     1621 gagaacagcg aagagaccag ggataatcga gagccagaag actgtctgga tactgtcgtg
     1681 aaaaatagcc ataaggacaa aggcaaaccc agggccgatc accgcgccca ccgtgtccat
     1741 ggagcggtga aagccaaatg cctttcctaa gatctctctt cctgtggagt ccgcaatgat
     1801 ggcatcccgc ggagcggtgc gtaccccctt gccaaagcgg tccgtaaacc tgtaaataag
     1861 gacatgcacc caggatcctg cggtggcaag gataggcctg ctgaacacag atatcccgta
     1921 tccccaaaat ataagggcct tacgtttacc caccctgtcc gagagccagc cggagaagac
     1981 cttgaggata cttgcggtgc tctctgcaat cccctcaatg accccgatgg atgtcttgga
     2041 tgcatggagg acattgctca ggtaaagtgg gataaggggg tagatcattt cagaactcac
     2101 gtccataaac aggctgacta ttccgaggac aaagacattc cttgggaaac cgaggatctc
     2161 cttcatggtg tgatattata aaccatgata gagatcgatg gaagttttgg agagggaggc
     2221 ggacagatac tcagaaccgc cctcagtttt tcctgcctct ttcacaagcc cttcaggata
     2281 ttcaatatca ggaggagccg taaaaaacct ggtctcatgc cccagcacct tacaggggtc
     2341 agggcagcac agctcctctc ccgtgcaaaa gtgcagggtg accgcgcagg ctcggtcgaa
     2401 ctctccttct cccccggaga ggtgaaggga ggaagttttt tctttgacat cggcacagcc
     2461 ggctcgacaa tgctcgttct ccagaccctt atccctgcct taattttttc aggagaaaag
     2521 accatcctca cccttgcggg cggcacacat gttcccttca gcccttcgtt ccattatgta
     2581 agagacgtgt ttcttccttt tttgaaaaaa gcaggaatag agatagccct ctctgtcgaa
     2641 tcctacgggt tctatccaaa aggcgggggc agaatacgtg ttgaattgtt tccaacgaag
     2701 gaggtgatgc ctttacacat tacggaaagg gggaagatgc tcagggtgaa gggaatatcc
     2761 ggtgtagcaa atctcccttc atccatcgcc gaaaggcaga ggaatgcggc acttggaaaa
     2821 ctcctccccc ataacactga ttgcggatat cagacggaca ttgaactgct ggaagtaccc
     2881 tcaccggggc aggggacgtt cattttcctg aaggcagaga gtgaacactc catcgcgggg
     2941 tttaccaccc ttggtgaaag gggcaagagg gccgaagcag tgggggaaga ggctgcgacg
     3001 gaactccttg aatacctcag gacagatgca gcccttggtc cccatctggc agaccagata
     3061 gtgccctacc tctccctttg caaagaacaa tccatattca ccacatgccg catcacccac
     3121 cacctgctca caaacctacg ggtaatcggt ctgttccatg aatttcagta ctctatcaaa
     3181 ggcgaaatcg gaatgcccgg gaccgtgaga atcaattaac ccggcagtcc tcgcgcaaat
     3241 tacttctcca caatcttgac cttcatctct acccgttcat tggggttgtc tctcgggtca
     3301 cggggctggc tcacaatctt gtcagccaca tccatgcctg agaccacctc accaaatacc
     3361 gtatactgtt tgttcaggaa aggcgcatcc gcaacgcaga tgaagaactg cgagcccgcg
     3421 ctgtcaggat ttgctgccct cgccatggac agcgttcccc tcttgtgggg cttgtcattg
     3481 aactcggcct tgacggtata tcccggcccg cccataccgt gttttgactt atccggattc
     3541 ttcgtgttcg gatctccccc ctgaatcatg aacccaggga ttacgcggtg gaaggtggtg
     3601 ccgtcataga atcccttctt cgcaagctcg atgaagttgt tcacatggtt tggagcaacc
     3661 tcgggaaaga acttcagctc aattgtacca agctttgtct cgatcaccgc ctttgtctct
     3721 gccatcttct tgatctcctc tgctgtaaat tttttttctt tcacctctgc gtatccaaaa
     3781 actaccgcac ccaagataat cattaccgca aggcttatgc cgacaatttt catgtctcct
     3841 ccgcaaagaa cattttttta ttgtgtatta taacaaaatt atttttttgt tattctctcc
     3901 gccaccttta aaaaagcact gccgtcgaat tataatagta tctctgtcag taaggtgtgt
     3961 gcactgaatt atgaaaaaga aaaacgtgtg tatagggata gcttgtcttt ttgtcttctg
     4021 cgcttccatg gtgagcggga catctcttga tgccaaaaca cctgagagcg tggagagcca
     4081 gaaaaaagca gtggtagcag tctctgtgga tgacaggtat ggaaggcgca ttgtctcggg
     4141 aagcggtttc attgtggacc gggagggggt ggtcgccacc agctgtaacc tcatcactaa
     4201 gttcctcgaa tcacaggaaa acaccatggc agtggaattg gc
//
LOCUS       DATKOU010000138         4589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3259502, whole genome shotgun sequence.
ACCESSION   DATKOU010000138 DATKOU010000000
VERSION     DATKOU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3259502"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1152)
                     /locus_tag="VMU21_06290"
     CDS             complement(301..1152)
                     /locus_tag="VMU21_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HUO77172.1"
                     /translation="MEDLLEHLKGFVLALISVICVLGIVVGYKYYKHTQEDPEFCMSC
                     HMMKEAFYEWQRGKHRDIVCQKCHHLTLIEQNQLLVSYVVKGKGEFSQTHGRKKPWVE
                     CRNCHLSEVSQGSVTMKKSHGHAKHVFALDMECKVCHGSSLHSFNPDENACRNCHKDK
                     GVHGIGMEAFSCLKCHSFAEKSSSMVSREKCLGCHRVPLRGPMAGFQCHQCHKPHGKI
                     KLKSADCLGECHTNETKVGQHNLHMKKGMECLNCHKAHTWIVGTAQAKTLCTRCHKFK
                     EPKKFVY"
     gene            complement(1211..1978)
                     /gene="lgt"
                     /locus_tag="VMU21_06295"
     CDS             complement(1211..1978)
                     /gene="lgt"
                     /locus_tag="VMU21_06295"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HUO77173.1"
                     /translation="MHPVLVRFGPLTIHTYGVLVAAGFLLGLALAVKQAKKEGIPQER
                     IIDIGFYVLVAAIIGSRLLFVAVNAGHYLKHPLDIFKIWEGGLVFYGGLILAIPSALW
                     YIRKHNLDAWRITDIFAPSLAIGHAVGRIGCFAAGCCYGRLSELPWAVTFHDPECLAT
                     TGIPLHPTQLYESAGELLNFLILITLRRYQSFKGQLFWTYILLYSLLRFVVEFFRGDE
                     ARGYLFGGISVSQGISVIMGVVAISVIAGKLLNKKGA"
     gene            complement(1978..2436)
                     /gene="lspA"
                     /locus_tag="VMU21_06300"
     CDS             complement(1978..2436)
                     /gene="lspA"
                     /locus_tag="VMU21_06300"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUO77174.1"
                     /translation="MKSPLYFLIAILVVLLDQITKLLARSFIGPFDTIKVSPFLHLVS
                     VRNEGAAFGLFKGFGNIPFIAISLIAAMVVIVLLVRGKEERFGLALILGGAIGNMIDR
                     ITMGSVTDFVDVFVGRFHWPAFNVADSALTVGLVVMVVGSFIHLKQKEKF"
     gene            complement(2433..>4589)
                     /gene="ileS"
                     /locus_tag="VMU21_06305"
     CDS             complement(2433..>4589)
                     /gene="ileS"
                     /locus_tag="VMU21_06305"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HUO77175.1"
                     /translation="ADKESPSVYVKFRVPDADVEHSIPRLKGRNVFFIIWTTTPWTLP
                     ANLALAVNPDYEYSALLVNGDVFIIAGELKDRLPFHGDVLLSVQGRTLEGMRAEHPFV
                     KRQSKVILGDFVTAGEGTGIVHIAPGHGEDDYEAGLKYGLDIYAPVDDKGRFTKAVPE
                     FEGQFVFKANDAIVETLKGKNALLAVQKITHSYPHCWRCKKPVIFRATEQWFISMEKE
                     QLRYRCLEEINRVNWIPQWGKDRIYGMVSNRPDWCVSRQRSWGVPITLLKCDQCGEFV
                     EDHELLDRIVRLVESHGSDIWFLKTADELIPPGYTCKKCGSSSFIKETDILDVWFDSG
                     VSHAAVMAKDGRLSWPADMYLEGSDQHRGWFQSSLIASAGTTNAAPYRTVLTHGFVVD
                     GQGKKMSKSLGNVVAPQDVIKKNGAEIVRLWVSAEDYRDDIKISQEIIDRLVEAYRKI
                     RNTARFLLGNLSGFDGGDYSGHMLEIDRWAMSRLQGLVKKVTEAYEKFEFHQVYHSIH
                     NFCIVDMSSFYLDILKDRLYTFRSDSPERRAGQWVLSQILSTMTRLMAPVLSFTAEEI
                     WSFMQENRADSVFLSPFPAVDDAFLEPELEERWERLRAVRDGVNKALEMKRQERFIGN
                     SLEAKVVLFTGDEEYSLLTTYRDILPTLFIVSDVEIEKGTAPEREVYENSVPKFSVLI
                     QRASGKKCERCWNWSDMVGAFHDAPELCDRCYNTLK"
BASE COUNT         1124 a         1216 c         1128 g         1121 t
ORIGIN      
        1 tatgtcctcc tttattttga cttcacccca tataaagcac cttccatgcc agattgaaac
       61 atattgaaat aaaagagtat taaaactaaa ccttttgcag aattgagaaa attatgcctt
      121 tcttttgcaa atgtgcaaaa aaagaactct tcttaccaga gattgaggat tgtgggagtt
      181 gaaacggatg agccttttac tgaatgagca ggaagacaaa gaagacctgc atccttattg
      241 atgagaaatc gggaaagtca aaccgcgaag caactacatt tgatattgag tttgttcaga
      301 ctagtatacg aacttcttag gctctttgaa cttgtggcag cgggtgcaga gggttttcgc
      361 ctgagcggtg cccactatcc aggtatgggc cttgtgacag ttaagacact ccatgccctt
      421 cttcatgtgg aggttgtgct ggcctacttt tgtttcattg gtatggcact ctcccaaaca
      481 gtccgctgat ttgagtttga tttttccatg gggcttatgg cactgatggc actgaaagcc
      541 tgccatgggt cctctgagag gcaccctatg gcatccgagg cacttctccc tcgagaccat
      601 gctggatgac ttctcggcaa aggagtggca cttcaggcag gaaaaggcct ccatcccgat
      661 accatggacc cctttatcct tgtggcagtt ccggcacgcg ttctcatcag ggttaaagga
      721 gtggaggctg ctgccgtggc acaccttgca ttccatatcg agggcaaata catgtttcgc
      781 atggccgtgg gatttcttca tggtgacaga accctgcgaa acctcgctca gatggcaatt
      841 cctgcactcg acccagggtt tctttctccc atgggtttgc gaaaactctc ccttgccttt
      901 aaccacatac gaaacgagga gctgattctg ttctatcagg gtgaggtggt ggcacttctg
      961 gcagacgatg tccctgtgct ttcccctctg ccattcgtag aaagcctcct tcatcatgtg
     1021 gcatgacatg cagaactcgg ggtcttcctg tgtatgtttg taatatttat accccaccac
     1081 gatcccgaga acacaaatga cggagattaa ggcaaggaca aaccccttga ggtgctccag
     1141 gaggtcttcc attagctttc cgaatttatt gtggaacctt ctcttcatta cttaaagaag
     1201 gcgacgaacc ctacgccccc tttttattga gcagtttccc tgcaatgacc gaaatggcga
     1261 caactcccat gattacactg attccctgag agacagaaat tccgccaaag aggtatcccc
     1321 gggcctcatc gcccctgaaa aactccacga caaatctcag taaggaataa agcaaaatgt
     1381 aggtccagaa caactgcccc ttaaaggact gatatctcct gagggtaatg aggatgagga
     1441 aattgagcaa ttctcctgcg gattcataca actgggtcgg gtggaggggg attccggtgg
     1501 ttgcgaggca ttcagggtcg tgaaatgtca ctgcccaggg aagttcagaa agcctgccgt
     1561 agcagcaccc tgcggcaaag cagccaatcc tccccactgc gtggccaatg gcgagagaag
     1621 gtgcaaagat atcggtgatg cgccacgcat ccaggttatg tttccttatg taccagagtg
     1681 ccgagggtat ggccaggatg aggccgccgt aaaatacaag acccccttcc catatcttga
     1741 aaatgtccag gggatgtttg agatagtgcc cggcattcac tgctacaaac aaaagccgag
     1801 accctatgat tgcagcaaca aggacataaa agccgatgtc aatgattctt tcctgaggga
     1861 tcccctcctt ttttgcctgc ttcactgcaa gggcaagacc cagcaagaac cctgctgcaa
     1921 ccaatacgcc gtaagtatgg atagtgagcg gaccgaagcg gaccagtacg ggatgcatta
     1981 aaacttttcc ttctgtttta gatggatgaa cgagcccacc accatcacca ccagtccgac
     2041 ggtaagggcc gaatcagcaa cattaaatgc aggccagtga aatctgccca caaacacatc
     2101 cacaaagtcg gtcacacttc ccatggtgat tctgtctatc atgttcccta tggctcctcc
     2161 caggatgaga gcaagaccaa accgctcctc cttgcccctt accagcagga ctatgaccac
     2221 catggccgca ataagggaaa tcgcgatgaa cggaatgtta ccaaaccctt tgaagagtcc
     2281 gaacgcagcg ccctcgttcc ttacgcttac cagatggagg aagggagata cctttatggt
     2341 gtcgaaggga cctataaagc ttcttgcaag gagttttgta atctggtcca ggagcaccac
     2401 cagaatggcg atgagaaagt agagagggct tttcatttca aggtattata acacctatcg
     2461 cagagctcag gtgcgtcatg gaatgcgccc accatatcac tccagttcca gcacctctcg
     2521 cattttttcc ccgatgctct ctggataagt acggaaaatt tcggtacgga gttttcgtaa
     2581 acctcgcgtt ccggggcagt acccttttct atttccacat cagaaacaat gaagagtgtg
     2641 gggagaatgt cccggtaggt ggtgagaagg gaatactcct catccccggt gaagaggacc
     2701 acctttgcct caagggagtt tccgatgaag cgttcctgcc gcttcatctc caatgctttg
     2761 tttaccccgt ccctgactgc cctgagcctt tcccagcgct cttccagttc aggctcaaga
     2821 aaggcgtcgt caacagcggg gaagggggag aggaagacgc tatccgctct gttctcttgc
     2881 ataaaactcc atatctcttc agcggtgaag gagagcacgg gcgccatcag cctcgtcatg
     2941 gtcgacagta tctgggataa cacccattgg cctgcccttc tctcgggaga gtcggaccgg
     3001 aacgtataga gccggtcctt gaggatatcg aggtaaaagg agctcatatc cacgatacag
     3061 aaattatgga tgctgtggta cacctggtga aattcaaatt tctcgtacgc ctcggtcacc
     3121 tttttcacaa gaccctgtag ccgcgacatc gcccacctgt caatctccag catgtggccg
     3181 gaataatccc caccgtcaaa accggagagg ttacccagta aaaaccgcgc ggtgttccgt
     3241 atcttacggt atgcctcaac gagcctgtcg atgatctcct gggagatttt tatgtcgtcc
     3301 ctataatctt ctgcagatac ccagagccgg acgatctcag caccgttttt cttgattaca
     3361 tcttggggtg cgactacgtt tcccagggat ttggacattt ttttcccctg tccgtccacg
     3421 acaaagccat gggtgaggac cgtgcggtaa ggcgcggcat ttgtggtccc tgcagaggcg
     3481 atgagggagc tctggaacca cccccggtgc tggtcactcc cttcaaggta catatccgca
     3541 ggccatgaga gtctcccatc ctttgccatg actgcggcat ggctcactcc ggagtcgaac
     3601 cagacatcca ggatatccgt ctccttgatg aaagaagaac tgccgcattt tttgcacgta
     3661 taccctggag ggatgagttc gtcagctgtt ttgagaaacc agatgtcaga gccgtgggac
     3721 tcaacgagac gcacgatacg gtcaaggagc tcatggtcct ccacgaactc cccgcattgg
     3781 tcgcatttta gtaaggtaat gggtacgccc caggaccgct gtctcgagac gcaccagtcg
     3841 ggcctgttgg ataccattcc gtaaatcctg tccttacccc attgggggat ccagttcacc
     3901 ctgttgatct cttcaaggca tctatatctc agttgctctt tctccataga gataaaccat
     3961 tgctctgtgg cgcggaagat caccggtttc ttgcacctcc agcagtgggg ataggaatgg
     4021 gtgatcttct ggacagcgag gagggcgttc ttccccttga gggtctctac aatggcatca
     4081 ttcgccttga agacgaactg gccctcgaac tcggggaccg ccttggtgaa tctgcctttg
     4141 tcatccaccg gtgcatagat atcgagaccg tatttgaggc ctgcctcgta gtcatcctct
     4201 ccgtgtccgg gagcaatgtg cactatcccc gtaccctctc ccgcagtcac aaaatcaccc
     4261 aggatcacct tagattgcct tttgacaaaa gggtgttctg cgcgcatccc ctcgagggtt
     4321 ctcccctgaa cagagagcag aacatcccca tgaaagggga gtctatcctt aagttcaccg
     4381 gcaatgatga atacgtctcc attgaccaag agcgcggaat attcatagtc cggattgact
     4441 gcgagggcaa ggtttgcagg aagtgtccat ggggtcgtgg tccagatgat aaagaaaaca
     4501 ttcctccctt taagcctggg gatggaatgc tccacatccg cgtcagggac cctgaacttc
     4561 acatagaccg acggggactc cttgtctgc
//
LOCUS       DATKOU010000139         7801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2492633, whole genome shotgun sequence.
ACCESSION   DATKOU010000139 DATKOU010000000
VERSION     DATKOU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492633"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VMU21_06310"
     CDS             complement(<1..598)
                     /locus_tag="VMU21_06310"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUO77176.1"
                     /translation="MKRYAVSVVLGASLILLSACGHTSGGSTNNAPSATGSVRAVFAG
                     TSAALIPWVDDPDRWETFSFQVLSQPINGTASVVSNQLLYTPSSAFKSGTDNFTYQAT
                     DHGGATVTGVAAVRVFSSTALSVCATTGTVNADGTINTRTKSNQCTFYSVGTTRVTNN
                     GTGVTMDYFINWPSNASAPKAVVVLIGGANLNMNFSGDT"
     gene            complement(962..1035)
                     /locus_tag="VMU21_06315"
     tRNA            complement(962..1035)
                     /locus_tag="VMU21_06315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(999..1001),aa:Arg,seq:acg)
     gene            complement(1043..1615)
                     /locus_tag="VMU21_06320"
     CDS             complement(1043..1615)
                     /locus_tag="VMU21_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HUO77177.1"
                     /translation="MRRVLAVSMGIALVFSLGLTACKKKEQQPVPQAPGQMPPSGVMP
                     PGQMPPGGAMPPGQMPPGQGGISVPKGEMKIIVPDSVKGKWSGVVLIVEDKSTKKKQE
                     FKVNLNSDLKIPNSNLKVAVGDFLPSFKMDGSTITSASNDPGNPAVRVKVYEGDKEVF
                     KGWLYSKFPAIHPFEHPKYGLGLKEGIKKG"
     gene            complement(1668..1757)
                     /locus_tag="VMU21_06325"
     tRNA            complement(1668..1757)
                     /locus_tag="VMU21_06325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1720..1722),aa:Ser,seq:gct)
     gene            complement(1814..2185)
                     /gene="nifU"
                     /locus_tag="VMU21_06330"
     CDS             complement(1814..2185)
                     /gene="nifU"
                     /locus_tag="VMU21_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HUO77178.1"
                     /translation="MYSEKVMDHFTNPRNVGEISDADGVGTEGNPTCGDVMKISIKVE
                     NGKIVDAKFKTFGCGAAIAVSSMVTEMVKGKTLEEALAISKEAVANELGGLPPQKMHC
                     SNLGADALRKAIEDYRSKHPA"
     gene            complement(2204..3430)
                     /locus_tag="VMU21_06335"
     CDS             complement(2204..3430)
                     /locus_tag="VMU21_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HUO77179.1"
                     /translation="MRKCYFDHVATNPLHPQVLDAMLPYLREEFGNPLSLYELGMNAR
                     EAIENARAQTSSLINSKPSELIFTSSGAEANNFALRGIALARQNEGKHVIVSRMEHHS
                     ILNAARFLEKSGFTVTYLPADKHGMVEPDAVKKAIIKETVLLSVVHASSEVGTIEPIR
                     EIAKIAREHGVIFHTDAVASTGNIPVDVKELGVDLLSMAAHQFYGPKGAGALYVREGL
                     RIVPLIYGGIQEGGRRAGTENVPAIVGMGKAAELAKAEMKGRMEHVKRLRDRLISGVL
                     GVENVYLTGHPADRLPGHASFAVEYIEGESMLLLLASKGMYVASGSACSSKALKASPV
                     LLSMGIPSSLAQGSIVFTLGKENTDEDIEYLLTEFPPIIKRLREISPFAKGWEEDAQG
                     SKCYTTFPESQKNGKD"
     gene            complement(3433..3687)
                     /locus_tag="VMU21_06340"
     CDS             complement(3433..3687)
                     /locus_tag="VMU21_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HUO77180.1"
                     /translation="MTLKFVENVKPDVTTDIVYMMCPMHLLKLEEKMKELEKGQILEI
                     MTDYDGALEDIPGWCEKTGNEFLGIDETDDYYKFYIKKVR"
     gene            complement(3684..4145)
                     /locus_tag="VMU21_06345"
     CDS             complement(3684..4145)
                     /locus_tag="VMU21_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HUO77181.1"
                     /translation="MLRLSTKGQYGVRAMFEIARGYPENPLTIKEISERQDVSVAYLE
                     QILNKLRKAGIVRSVKGPGGGYLLAREPGHVTIAEILKELEGPVAITSCLDPEEGCSR
                     VEGCVTHLLWRSLGAKIEAFLETITLKDLLEKEPILKGVDHDDSLYGFDVA"
     gene            4223..5605
                     /locus_tag="VMU21_06350"
     CDS             4223..5605
                     /locus_tag="VMU21_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77182.1"
                     /translation="MLPLLFGVYGILNHANGTWGKGTRDNSVNVVAMVINLNMPAIEN
                     LGLGTVPQTDSPRAGLSVLMLILLVLLLISVFFAAVFIYRNIIGKRRQTTLKEDYRKE
                     AEHYEKSGRFVSAAALYENQLKDGEKAAELYERGGDFRRAALLYNLLGVPDRAINMYM
                     KDGDIQSAAEVSVLEGNYEEAAKLYYEAGKKIDAALILEKAGRKMAAARTYREAGEYK
                     KAAQLLEEEGMHREAAEMFGIALTDKTIEGHIDDFYTYALKLERAGERQKALRVFQEI
                     DRANPLYRDVREKLGTLALPLDEGSAESKTTLRNFIRSGKIEPRHALKLWVHILKALQ
                     EAYKDGKSHGPLSPDTIAIDTRNRISFLFGPVSTVYASPETLKGAEPDACSDIYTAGI
                     ILYEMLMGDLDGLGSARIIERVEDVPEWLDELVLKCIRKVREDRYRSIEAIFGEVKAL
                     SKGKGSHGME"
     gene            complement(5705..>7801)
                     /locus_tag="VMU21_06355"
     CDS             complement(5705..>7801)
                     /locus_tag="VMU21_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO77183.1"
                     /translation="LAHLMEEIRRDPDRFKDSENENITKDGRRVWVRWANKAIVDGQG
                     MLVGILSIGNDITERRRAEEEKDRLAKAVSIVTEGIAITDERDRYIYLNDTHARTYGY
                     LQSELLGKTWRDLTPSELVPLLEREISRTLHSRDIGIWSGECPGLSKDGTVISTEVTA
                     TARWNEKGDYLGHICVVRDITERKRAEERLRESEERFRDLFENATDLIQIVSPEGRIL
                     YVNRSWRETLGYSEGEIRELRIPDIIDPACDEYCKGTFGKILSEGSVEDIKTIFIAKD
                     GRKVFLEGAGRCKYVGGSPESVRCMFRDMTFRKAAEEDRARLEAQLLQAQKMEAVGQL
                     AGGIAHDFNNILTAIIGYASLLEAKLGTDSPLKPYVEQILTSSEKSANLTSQLLAFSR
                     KQIMSPKETDVNELIRGMEILLKSLIGEDVEFKTCFTDMHLTAMVDRGQIEQVLLNLC
                     ANARDAMPHGGLLAISTEVLELDEKSIRIYDLDQPGRYGLISVSDSGHGMEEMTRERI
                     FEPFYTTKEMGKGTGLGLSIVYGIIKQHNGNITVYSEPGKGTTFKIFLPLIVSPVETA
                     NKESLVDPRGGPEAILVSEDDEAVRSLTSHVLQEAGYTVIEAVDGDDAINKFMENRDR
                     IKLAILDVVMPKKSGKEVSDVIRKTKSDIRVLLTSGYTADIISTKGISEEGLDFISKP
                     ITPNNLLRKVREVLDA"
BASE COUNT         1858 a         2114 c         1757 g         2072 t
ORIGIN      
        1 tagtatctcc tgagaagttc atgttgaggt ttgccccgcc aatcaatacc accactgcct
       61 taggagcgct cgcatttgat ggccaattga taaaataatc catcgtcacc ccagttccgt
      121 tgttagtcac acgagtcgta cccacgctat agaaagtgca ttgattggac ttagtgcgtg
      181 tattaattgt gccgtcagca ttgacagtac cggttgtcgc gcagaccgag agtgccgtgc
      241 tggagaagac tcgaactgcg gcaactcctg tgaccgtggc accaccatga tccgtagcct
      301 gataagtgaa attgtcagtt ccgcttttga aagcagaact aggcgtgtac agcaactggt
      361 tactgaccac agatgctgtc ccgttaatgg gctgactcag tacctgaaag ctgaaagttt
      421 cccacctatc aggatcatcc acccagggaa tcagagcagc tgacgtgcct gcgaatacgg
      481 cacgaacgct gcctgttgca gaaggggcat tattagtaga gccaccgctc gtatggccac
      541 aagcacttaa taaaatcaag gaagccccta aaacaaccga tactgcatac cttttcatgg
      601 tgttacctcc tttacgtctt caatttaaaa gccaaactca cagaaggttt atctttcgag
      661 aactccggta tcatggccgc aaaaaaagac agcacggtga aagttttcat ccatatgtca
      721 tcagatactt accccttata tagacgctga gaccaagtga aaggttacac aggaaggtgt
      781 tcgagaagca gccatcgaaa tatcgaacac tgtcgacaca gtcagggcgg aaagaaccct
      841 gagatatgct cacctttgac aaaccaagtg caaaattagt gcataaaaga gaggctgcaa
      901 gccatgtcta gcacttacaa cccctcttct aagtctttga atctcttagg gtttatggct
      961 acgcgccctg agggattcga acccccgacc tgcggattcg tagtccgacg ctctatccag
     1021 ctgagctaag ggcgctgcct ctctaaccct tttttattcc ttcttttagg ccaagaccgt
     1081 atttcggatg ttcaaaggga tggattgcag ggaacttgga gtaaagccag cccttgaaaa
     1141 cctctttgtc accctcatag acttttaccc tcaccgccgg attaccggga tcattggatg
     1201 cagaggttat ggtacttcca tccatcttga aactaggaag gaaatcaccc accgccacct
     1261 tcagattcga attgggtatt tttagatcac tgttaagatt gaccttaaat tcctgttttt
     1321 tctttgtgga cttatcctcc acaatcaata cgactcctga ccattttccc tttacgctat
     1381 ccgggactat gatcttcatc tcaccctttg gcaccgatat accaccctgt cctggaggca
     1441 tctgtccagg tggcattgct ccgcctgggg gcatctgtcc gggcggcatt actccgcttg
     1501 ggggcatctg tccgggagct tggggcacag gctgctgttc cttcttttta catgcggtga
     1561 gaccgaggga aaacacaagg gcaattccca tactaactgc taagactctt ctcattttga
     1621 gacctccttt tcgggtttag ttcttaaaag taattcacac ttcactacgg agaggcaggg
     1681 attcgaaccc tgggtggggt gttaccccca caaccgctta gcaggcggct gccttcgacc
     1741 agctcggcca cctctccagg aactggttat tgtaacacct tgaatccggc tttgtaaatg
     1801 aagctatgtg cgcttacgca gggtgttttg aacgataatc ttcgatggcc tttctgaggg
     1861 catcggcacc gaggtttgag caatgcatct tctgcggggg aagaccgcca agctcattgg
     1921 ctacagcctc cttggaaatc gccaatgctt cttccagggt cttgcctttc accatctcgg
     1981 tcaccatact gctcaccgca atagctgccc cgcaaccaaa ggtcttgaat tttgcgtcca
     2041 caatttttcc gttctcgacc ttgatcgata tcttcatcac atcaccacag gtagggttcc
     2101 cctctgttcc cacaccatca gcatccgaaa tttctcccac attccttgga ttggtaaagt
     2161 gatccattac tttttcgcta tacatgatac ctcctcctct atttcaatcc tttccgtttt
     2221 tctgtgattc cggaaacgtg gtataacatt tgctcccctg tgcgtcttct tcccacccct
     2281 tcgcaaaagg ggatatctcc cttaatcttt ttattatagg cggaaactcc gtaaggagat
     2341 actctatgtc ctcatcggta ttttccttgc caagggtaaa gacaatggag ccctgcgcaa
     2401 ggctagaggg tatgcccatg gagagcagca caggagaggc cttcagcgcc ttggaagagc
     2461 atgcagagcc gctcgccaca tacatgccct tggatgcgag gaggaggagc atggattccc
     2521 cttcaatata ctcaaccgcg aagcttgcat ggccgggcag ccggtctgca ggatgtccgg
     2581 tgagatagac attctccacg cccagaacgc cgctgatgag cctgtcccgc agtctcttca
     2641 cgtgttccat tctccccttc atctcagcct ttgcgagctc tgcagccttt cccattccca
     2701 ctatggcagg gacattctct gtgcctgccc tcctaccccc ttcctgtatc ccgccgtata
     2761 tcaaaggaac aatcctcaac ccctctctca cataaagggc acccgcaccc ttgggtccgt
     2821 agaactggtg agccgccatg ctcagaaggt ctactcccag ttcctttacg tcaacgggga
     2881 tattcccagt tgatgcaacg gcatctgtat ggaatatcac gccgtgttcc ctcgcaatct
     2941 ttgcaatctc cctgatgggc tctatggttc ctacttcact gctcgcatgg accacagaaa
     3001 gaagaacggt ttccttgatg atcgcctttt tcaccgcgtc aggttccacc ataccatgct
     3061 tgtccgcagg gaggtaggtg accgtaaacc ccgacttctc caggaacctt gcggcattga
     3121 ggattgagtg gtgctccatt ctcgaaacga tcacgtgttt tccctcattc tgtctcgcaa
     3181 gggcgatccc cctcaatgcg aaattgttcg cctccgcgcc actgctggtg aatatcagct
     3241 ctgagggttt tgaattgata agggacgagg tctgggccct ggcattctca atggcttccc
     3301 ttgcgttcat gcccaactca tagaggctga ggggattgcc gaactcctct ctcaaatagg
     3361 gcagcatcgc atccagtacc tggggatgca ggggatttgt cgccacatgg tcgaagtaac
     3421 attttctcat aattacctca ctttctttat atagaactta tagtaatcat cggtttcgtc
     3481 aatcccgaga aactcgttcc cggtcttttc acaccatccc ggaatgtcct ccagcgcacc
     3541 gtcatagtct gtcatgatct ctaatatctg tcccttttcg agctccttca tcttttcttc
     3601 caacttcaaa aggtgcatag ggcacatcat atacacaata tctgtggtga cgtccggctt
     3661 aacgttttca acaaacttca gtgtcatgcc acatcaaacc cgtagaggga atcgtcgtgg
     3721 tcaactccct tgagaatagg ctccttctcc agcaggtcct tcagtgtgat ggtctccaga
     3781 aaagcctcaa tttttgcgcc gagagatctc cagagcagat gagtaacaca gccttccacc
     3841 cttgagcagc cctcttcagg atcaagacat gaggtgatgg caacaggccc ttcaagttcc
     3901 ttgagaatct cggcaatggt gacatgaccg ggttccctgg cgaggaggta tcctcctccg
     3961 ggacctttca cgctccgaac aatacccgcc tttctgagtt tgttcaatat ctgctcaagg
     4021 tatgccaccg acacatcctg ccgctcagaa atctccttta tcgtcagagg gttctcagga
     4081 taaccccggg cgatctcaaa catggccctt accccgtact ggccctttgt tgacaaccgt
     4141 aacatgataa aaatataaaa tactctacta attttgtcaa gtattagcac aaaagaaaaa
     4201 gaggcggttg aggatgttgt ttttgcttcc cctactcttt ggcgtgtatg gtatattgaa
     4261 ccatgcaaat ggtacatggg gaaaaggcac tcgtgacaat tccgtgaatg tagttgcgat
     4321 ggtcataaac ctcaatatgc cggccattga aaatctcggc ctcgggacag taccccaaac
     4381 agacagcccg agagcaggcc tttctgtcct catgctcatt ctccttgttc tcctgctcat
     4441 ctcggttttc tttgcagccg tctttatcta caggaatatc ataggcaaga gacgtcaaac
     4501 tactttgaaa gaggactaca ggaaagaggc cgagcactac gaaaaatcag gcaggtttgt
     4561 atcagccgct gcactctatg aaaaccaatt aaaagatggg gaaaaggcag cagaactcta
     4621 tgagagaggc ggcgacttca gacgggcagc tcttctctat aatctcctgg gagtgccaga
     4681 tagagccata aacatgtata tgaaggatgg cgatatccag agtgctgcag aagtgtcggt
     4741 cctagaaggg aattacgagg aggccgcaaa gctctattat gaggcgggga agaaaatcga
     4801 tgctgcgctt atcctggaaa aggcgggaag gaagatggct gcggccagga cataccggga
     4861 ggcaggagaa tacaaaaagg ccgctcagct tctggaggaa gaagggatgc acagggaggc
     4921 ggcagagatg ttcggcattg ccctcactga caaaacaata gagggtcata ttgatgactt
     4981 ttacacatac gcgctcaagc ttgaacgggc aggtgagcgg caaaaggccc ttagggtatt
     5041 tcaggagata gaccgcgcaa accctctgta tcgcgatgta agggagaaac tcgggacgct
     5101 tgccctgccc ctggatgaag gaagcgccga atcaaagacc acattaagaa atttcatccg
     5161 cagcggaaaa attgaaccca ggcatgccct caaactctgg gttcatattc ttaaggccct
     5221 tcaggaagca tacaaggatg gcaaatccca tggtccgttg agccctgata ctatcgcaat
     5281 tgacacccgt aatcgcatct ctttcctctt cggaccggtg tccacggtct atgcctctcc
     5341 ggaaaccctc aagggcgctg aacctgatgc ctgctccgac atttatacag ccgggatcat
     5401 tctctatgag atgctcatgg gggatcttga cggacttggt tcagcaagga tcatagagag
     5461 agtggaggat gtccctgaat ggctggatga gcttgtgctc aaatgtatac gcaaagtgag
     5521 ggaagacaga taccggagca tagaggcaat tttcggtgag gtgaaagccc tttcaaaggg
     5581 aaagggctcc catggcatgg aataattatt tggtcattct attactcgcc cagttacttc
     5641 gattgacaag acaggatgta cggggaccca gtaaggttct tcacttgggc ttcgtcagtc
     5701 cctttcaggc atccaggacc tctctcacct ttcttaacag gttattgggc gtaataggtt
     5761 ttgatatgaa atccagcccc tcttcgctga taccctttgt gctgattatg tctgccgtat
     5821 aaccgctggt aaggagaacc cgtatatcag attttgtctt ccttatgaca tcgctcactt
     5881 cttttccgct tttctttggc atcaccacat caagtatcgc aagcttgatt ctgtccctgt
     5941 tttccataaa tttatttatg gcgtcatccc catctactgc ctctattacc gtatacccgg
     6001 cttcctgaag cacatgactt gtcagagatc gcaccgcttc atcatcttcg ctcacaagta
     6061 tggcctctgg tccgcccctt ggatcaacga ggctctcctt gtttgctgtt tccacggggg
     6121 acacaattaa cggaagaaaa atcttaaacg ttgtgccttt cccgggttca ctatacaccg
     6181 tgatattgcc attgtgctgt ttgattatcc cgtatacaat ggacaacccg agacccgtcc
     6241 cttttcccat ctcctttgtg gtatagaacg gttcaaagat tctctctctg gtcatttctt
     6301 ccattccatg accgctatcg ctcacggaaa tgaggccata cctgccgggc tggtctaaat
     6361 catatattcg tatgcttttt tcatccagtt cgaggacctc tgtactaatt gctagcaggc
     6421 ctccatgagg catggcgtcc cgtgcattgg cgcagaggtt gagcagaacc tgttctattt
     6481 gccctctatc taccattgcc gttaaatgca tatcggtaaa gcatgtcttg aattcaacat
     6541 cctcaccaat aaggcttttc aaaagtatct ccattcccct tataagttcg ttcacgtctg
     6601 tctctttagg actcattatc tgctttctgc taaaagcgag aagctggctg gtgagatttg
     6661 ccgacttttc tgaagaggtc agaatctgct caacataagg tttaagagga ctatcagttc
     6721 cgagttttgc ctccaggagg cttgcgtaac cgatgatcgc tgtcaagata ttgttgaagt
     6781 catgggcaat cccgcccgca agctgaccca cagcttccat tttttgcgcc tggagaagct
     6841 gtgcttccag tcttgccctg tcttcctctg cagccttccg gaaagtcata tcccgaaaca
     6901 tgcaccgaac cgattccgga cttcctccca cgtatttgca gcgacccgcc ccttccagaa
     6961 ataccttcct gccgtccttg gcgataaaga tggttttgat atcctcaaca ctcccttcag
     7021 acagtatctt cccaaaagtg cccttacagt actcatcgca cgcgggatcg atgatgtcag
     7081 gaattctcag ttctcttatc tccccttcgg agtatcccag cgtctcacgc catgaacggt
     7141 tcacgtacag gatacggccc tcaggagaaa cgatctggat gagatcggta gcgttctcga
     7201 agaggtcacg gaaacgctct tcgctctctc tcaatctctc ttcagcacgc ttgcgctcag
     7261 tgatatccct caccacacat atgtgcccca ggtagtcgcc cttctcattc catctggcag
     7321 tcgcagtgac ctcggtggat atcactgtgc cgtctttgct taagccagga cattcaccgc
     7381 tccagatacc gatatccctg ctgtgcagag tcctggagat ttctctctct agtaatggca
     7441 ctaactcaga cggtgtaaga tcgcgccatg tttttcccaa aagctcactt tgcagataac
     7501 catatgtcct ggcgtgagta tcattgaggt agatatatct gtctctttca tctgttatgg
     7561 cgatgccttc ggtaacaata gagaccgctt tggcaagtcg gtctttctcc tcttcagcac
     7621 gcctgcgctc agtgatatcg ttcccgatag agagaatacc gacaagcatc ccttgtccat
     7681 ccacgatcgc cttgttcgcc caccggaccc atacgcgccg gccgtccttc gtgatgttct
     7741 cgttttcact gtccttgaaa cggtccggat ctcttcgtat ctcctccatc agatgcgcaa
     7801 g
//
LOCUS       DATKOU010000140         9572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5572464, whole genome shotgun sequence.
ACCESSION   DATKOU010000140 DATKOU010000000
VERSION     DATKOU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5572464"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VMU21_06360"
     CDS             <1..631
                     /locus_tag="VMU21_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUO77184.1"
                     /translation="QAVKTGQLVVAIGNPFGFQCSVTAGVVSALGRSLRARSGRLIDN
                     VIQTDAALNPGNSGGPLVNFRGEVIGVNTAMILSAQGICFAIAINTAKFVAAGLMKEG
                     KIRRSYIGVAGQNIVLNRRMVHFHKLRVESGVMAVAIEINSPALRAGLREGDVIIGFG
                     DQPVAGIDELHKLLTQEKVGKSFPITLIRMTEKIVLDIVPEESSSRSYN"
     regulatory      751..834
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            827..1201
                     /locus_tag="VMU21_06365"
     CDS             827..1201
                     /locus_tag="VMU21_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HUO77185.1"
                     /translation="MPKKRLSVSSAFLFKKGKGANIMNAKVVIFQIKPGKQGEVVRLF
                     KEFVIPGAEKQKGFKGGLLLTDPNTGKATSIALWETEADIKASEASGYYKERVAKLSD
                     ALALPPVREIYEVNNLVNLSVK"
     gene            1370..2557
                     /locus_tag="VMU21_06370"
     CDS             1370..2557
                     /locus_tag="VMU21_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77186.1"
                     /translation="MYLTSFVHRDQLFSLAERWFSGRPEPGDVRALTEILICDVFIIR
                     ETLEVLSRRLLGMIHKGPLQQRRIRLKGDLRDLLSAVNRDSTPRTEELFSRYRQNPDF
                     YYRESPMNASVFLDGLGHILGVYRVKRPKRIAEKANRRIADWIFRNVLERAREMAMDR
                     ARQSGIPLEQFITPPGEMMEEFVRAEEHIAESFRDGSMRFEQSALTIQDVGALKIVAN
                     SGTLEQLEKSLSVASDILVVEKEHFSGNFRATNLILEVPWDPGYVSRQYRDTNYWEHY
                     RNRGIPFADLCKGLDPFFSGAKPTISIELILTTFPDLVESELGSSIHEERIISQRDNK
                     YYKGYIPMNVEFLVEFLFAVGFSPVDHLEEIPIKIWGRYLPETLGSYIRQLYALPDHD
                     FFG"
     gene            3080..3781
                     /locus_tag="VMU21_06375"
     CDS             3080..3781
                     /locus_tag="VMU21_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUO77187.1"
                     /translation="MSKKTAFWIFMIGTVFSAILFLGLTWDTHRQIVTLSHADKLSVD
                     VVKGKRVFEKYNCNDCHTILGFGGYYAPDLTKVVQRVGEEGIRYRVKNPGKAFETSWR
                     KMPNQKLSDEEIDHLVAFFTWVGNVDNGDWPPQDSKKRLTRSEERMVAGVGLSAGAAV
                     FQTRGCMNCHSLHGTGGSFGPALDTIAQKLTREQIEHYVKDPKSVNPKALMPAQKELS
                     DKELEEVAEFLANVK"
     gene            3791..5122
                     /locus_tag="VMU21_06380"
     CDS             3791..5122
                     /locus_tag="VMU21_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HUO77188.1"
                     /translation="MEYRSQKIAYWYFAIALSLFTLQVIVGLWLAFNYFFTVPQSIVD
                     KFPFATARAIHTNLLVLWMLLGFMGGTYYIVPEETGREIFSPRLAWFQLVALVVTGVV
                     ALVGFVFGWTQGRPLLEIPRPLDFVVVVGALIFLFNVGITMFKAKRWTAIQGTLLGGL
                     LFLALLYLFGIPFYENEVIDWYYWWWVIHLWVEGAWELVTAAIFAFILIKITGVDRQV
                     VEKWLYVEIGLFLFTGIAGTGHHYYWIGAPRYWLWVGGIFSALEPLPILLMLIDTMNH
                     VKHRQIKIINPLTWTYAIGCAVLHMIGAGVWGFMHTLPQINYYTHGSQVTVSHGHLAF
                     FGAYALLNLTVFYFAIPRIKGIEQYDARRGKVGFWTMVTAMTLMGLSFGIAGVVQAYV
                     ERVLGMGYMAAQSYMRLWMAVTFTLGLCFFVGVLITVTDLLTLRPARKVID"
     gene            5445..6053
                     /locus_tag="VMU21_06385"
     CDS             5445..6053
                     /locus_tag="VMU21_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HUO77189.1"
                     /translation="MEYDFLVDTYDIERLKTLNVWSMFRDEDLAIRPHPLLGRDRNPR
                     EHMVHQCVSENKWFCNMFGIDVGAPPLPAREVRLEFIKRYAEDSGKRLLILREKEKTW
                     WEQEAFFFDVKRTRTWIMVRRIAHTAHHRAEMTTLLRLLGRQVHSVYGPSVDTGGLPD
                     NKALTIYAYPDIDSLIEGESRGSSKKALPGPGDKPSTERPDF"
     gene            6121..7068
                     /locus_tag="VMU21_06390"
     CDS             6121..7068
                     /locus_tag="VMU21_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77190.1"
                     /translation="MAAAKEANVMKGLYFINTMALVAVLLLMPVVMHGQSGQTGTETP
                     PIGQPLAREGDLAVKVVEALGLGSTNDEVEAESMAGSVGIAPRNGWIADYPVTPDIIG
                     EVQDTIVYAADSGRISITRDDALNAYRQVVAEISLPVKSYTYGETCEAQDCRNYPNPT
                     TISNYYSDQGPPVVTYYTPPPDYYYLYGWVPYPFWWSGFWFPGFFVLHDFHKVIIVHN
                     RPVFVTNHFSRFGTPGVFRVDPVQRFNGRTFAGIGAPRSGRFLSTGIERAPHTVFNPG
                     RHQMMQGGGKMTTPSSGGRTAAPSSRSGGGSSQGGGMRR"
     gene            7722..9539
                     /locus_tag="VMU21_06395"
     CDS             7722..9539
                     /locus_tag="VMU21_06395"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HUO77191.1"
                     /translation="MIPPKEFIRKIHPFSFLSEDDLDIIVSGLEVDLFEKGKTIYKKG
                     QTSENVYVIFSGLVGLFDDEAPIDYLSRGEIFGLMSLYGNLLNATAKAMEDTVCYAVA
                     HRQFKIIFDSNERFASFFSTLLNRRLRSFKTIASDKKILEEALFVLELEKIIYKNPVV
                     CSPEMTIAGAAFEMDKNSVSSIVIVDTNAMPIGILTHKDLRKVLISGDRLDPVTEFMS
                     SPVKTINVQATIFEAYSKMIDAGIDHLVVTKNDKVFGIITRKDIQIHLEPSFSIVKLF
                     RKVHKAASIEELKTAFEGLMVSVSKIAMTGPNFFDLTRMLCSVHDAVVYKVIQILIDK
                     HAPARFLWIHMGSSGRKEEIIATDQDNALIYLGDNPAALAKNINESLAKIGYPTCFGN
                     YMASNSLWNQPLPVWKEYFNQWFSDPIPDHLRYLSVFLDMRPLYGETALYNELLESIK
                     AAVKAEAIKLMAEDTIEIRPPLGIFGILGLHKGVDLKTYGIFPIVNGARVLAVDSGVW
                     GITNTKERLEALSANMTISNEMCHDLIESYGFLQDLRLRHHARAVLNKSQANNLITTK
                     ELSNVDLLILKESLKIVSEFQKFLMKKYGVSRTVIYSQL"
BASE COUNT         2527 a         2126 c         2443 g         2476 t
ORIGIN      
        1 acaggccgta aagaccggac agttggttgt tgccatcggg aatccctttg gctttcagtg
       61 cagcgtgact gcaggggtgg tgagcgcgct gggtcgttca ctcagagcgc ggtccggaag
      121 actcatagac aatgtgattc agacagatgc agccctgaat cctggcaatt cgggcgggcc
      181 gctcgtcaat ttccgaggcg aagtaatagg agtgaatacc gcgatgattt tatctgcgca
      241 gggcatctgt tttgccattg cgataaatac tgcaaaattc gtagcagcag gacttatgaa
      301 agaagggaag atacggcgca gctacatagg tgtggcaggg cagaatatag tgctcaaccg
      361 gcgtatggtg cattttcaca agctgagagt cgaaagcggt gttatggcag ttgcaatcga
      421 aatcaacagc cctgcgctga gagcaggact gcgtgaagga gacgtaatta ttggttttgg
      481 agatcagcct gttgcaggga tcgatgagct gcacaagcta ttgacacagg agaaagtggg
      541 gaaaagcttt cccattaccc ttatcaggat gaccgagaaa atagttcttg atatagttcc
      601 ggaggaaagc agttcccgat catacaatta accaggtttt ctcttgcctc cttccctctg
      661 ataagggata ttgaatggtt ttatacttcc cattaaaaac attaaataga ctaaacagga
      721 ctattttggt acatcttaac taagaaagga ttccgacaaa gccaaaccct ctgtgaagaa
      781 gggacagaaa gcgagggatc ttatcaatgg aattaagatg gccgagatgc cgaagaaaag
      841 actttcggtg agttcggctt tcttatttaa aaaggggaaa ggagctaaca ttatgaacgc
      901 aaaagttgta attttccaga ttaagcctgg aaagcaaggt gaagttgtcc gtttattcaa
      961 ggagtttgtt attcccggtg cagagaagca gaaaggattc aagggaggac tgttgttaac
     1021 agatcccaat acaggcaagg ctacttccat agccctatgg gaaactgagg cagacataaa
     1081 ggcaagcgag gccagcggct attacaagga gcgggttgcc aagcttagtg atgcccttgc
     1141 tttgcctcct gtaagggaaa tatacgaggt gaacaacctt gtaaatctct cagttaagtg
     1201 accaagggag aaaaggtgga agggtaagat agctggatcg cgttaaccct atttgtatct
     1261 aagcctaaac ccatctccga tccacagact ttcttcagac ccttgttgta cttcctggct
     1321 gactggcgta tcatatctcc agtttactca agaagggaga ctccttcggg tgtatctcac
     1381 atcttttgtt catcgcgatc agttattcag cttagcagag cgctggttca gtggccggcc
     1441 tgagcctggt gacgtacggg cactcactga gatccttatt tgcgatgtct tcatcatcag
     1501 ggaaacgctt gaggtgctca gcaggcggct gctcggtatg attcataagg gtcctttaca
     1561 gcagagacgt atccgtttaa aaggcgatct ccgggatctc ctgagtgccg tcaacaggga
     1621 ctcaacaccg cggaccgaag agctgttcag caggtaccgg caaaaccctg atttctatta
     1681 ccgggaatca cccatgaatg cctcggtctt tctggatggc ctggggcata tactcggagt
     1741 ctatcgggtc aaacggccga agagaatagc tgaaaaggcc aaccgtcgca tagccgactg
     1801 gatattcaga aatgtgcttg aacgcgccag agagatggcc atggacaggg caaggcaatc
     1861 aggcatcccc ctggagcagt tcatcacacc cccaggggag atgatggagg agtttgtcag
     1921 ggccgaggag cacattgcag agagcttcag ggacggcagt atgcggttcg aacaaagtgc
     1981 gctgacgata caggatgtag gcgctttaaa gatagtggcc aactccggca ctttggagca
     2041 actcgaaaag agtctcagtg tggcttcgga catcttagta gtagaaaagg aacacttcag
     2101 cggcaacttc agggccacta acctgatact cgaagtccct tgggacccgg gctatgtctc
     2161 cagacaatac cgggacacca attactggga acattatagg aataggggaa taccgttcgc
     2221 agacctgtgt aagggtctcg atcctttttt ctcaggagct aagccgacga tcagcattga
     2281 gttgatcctc actacattcc ccgacctagt cgagtcagaa ttgggctcca gcattcatga
     2341 agagaggatc atctcccagc gggacaataa gtactataag ggttatattc ccatgaacgt
     2401 cgaattcctg gtggagttcc tgtttgctgt aggattcagc cctgttgacc atcttgaaga
     2461 gataccgatc aagatctggg ggaggtatct gcccgagaca cttggttctt atatacgaca
     2521 gttatatgcc ctgccagatc acgatttttt cggctgaagg catacactga aagtgcggat
     2581 tcgtcggtga aagcctcagt cgaaactctt tctgacattg tctgtcaaat caaatagtgg
     2641 cattcctgca ttggaaaatg ttctgtattt tctgtatatt gaggttgctt atgctctaat
     2701 tttgctgcac ccatcattcc tatcgaattc ttcagttccc cgttctggaa gttgtgtgta
     2761 tgggtctgct tttcaggagg ggcgacggtg ctcgcttcat ccaggactcc tcgctcccac
     2821 gtatcacagt cagtacgcat ctttttttca gcagtggcag taggatatgt tatgccaaat
     2881 aatatgtttg cataattacg catctcaaaa cgatgcaata cattgaaaat cctcgcggga
     2941 atgatagaat tcatggtgaa agggtttgtc tgcaagagaa ctgattcggg gagctaagat
     3001 cagaggctga aagggggtag gaaacacagt agagacgtag acataatctg tcagccttgt
     3061 aattatgagg aggtgtggaa tgtcaaaaaa gaccgctttc tggattttca tgatcggtac
     3121 cgttttctcg gcaattctgt ttcttggtct cacatgggat acccaccgtc agatcgtaac
     3181 gctgtcccat gcggacaagt tgtctgttga cgtagtcaag gggaaacggg ttttcgagaa
     3241 gtacaactgc aatgactgcc atacgatttt aggcttcggc ggctattatg cccctgatct
     3301 cactaaggta gtacagcggg taggagaaga aggcatccgt tatcgggtga aaaacccggg
     3361 aaaggccttt gaaacctcat ggcggaagat gccgaatcaa aaactctctg atgaagaaat
     3421 tgatcatctc gttgcttttt tcacttgggt gggaaatgtg gacaatggag attggcctcc
     3481 acaggacagc aagaaacgtc tcacaagaag cgaggagaga atggtcgctg gagttgggct
     3541 ttctgccggc gcagcggttt tccagacaag aggatgtatg aactgtcatt ctctccacgg
     3601 gacagggggg tccttcggtc ctgcgcttga tacaatcgcc cagaagctca cccgtgaaca
     3661 gattgagcat tatgtcaagg accctaaatc agtgaatccg aaagccctca tgccagcaca
     3721 gaaagaactc tctgacaaag aacttgaaga agtggccgag tttctggcca acgtaaaata
     3781 ggaggctaca atggaatacc gctcacagaa aatagcatac tggtactttg ccattgccct
     3841 ttccctgttc actcttcagg tgatagtggg gctttggctt gcgtttaatt acttttttac
     3901 ggtgcctcag tcaatcgttg acaagttccc ctttgctact gcccgagcta ttcacacgaa
     3961 tcttctcgtt ctctggatgc tcctgggatt tatgggtggc acctattata ttgtgcctga
     4021 ggaaacaggg agagagatct tttccccaag gcttgcatgg tttcagttgg ttgctcttgt
     4081 cgtgacaggc gttgttgcgc tcgtcggctt tgtcttcggc tggactcagg gacgaccgct
     4141 ccttgaaata ccacggcctc tcgactttgt tgtcgttgtg ggtgccctca tcttcctttt
     4201 taatgtcgga atcacaatgt tcaaggcaaa gcgctggacg gcgatacagg ggacacttct
     4261 cggaggtctt cttttccttg ccctgcttta tttgttcgga atcccttttt acgaaaacga
     4321 ggtgattgac tggtactatt ggtggtgggt catccatctt tgggtggaag gcgcgtggga
     4381 gctcgtaacc gcggcgatct ttgccttcat cctcatcaag attaccggag ttgaccgtca
     4441 ggtcgttgag aaatggctct atgtcgagat cggcctattc ctgtttactg gtattgctgg
     4501 cacgggccat cattactact ggattggagc gccgagatac tggctctggg tcggcggcat
     4561 cttctctgcc ttagaaccgc tccctattct tcttatgctc atcgatacca tgaaccatgt
     4621 caagcaccgg cagatcaaaa taataaatcc cctcacctgg acttatgcga tcggctgtgc
     4681 tgttctccac atgatcggag ccggtgtctg gggattcatg cacaccctcc cccagatcaa
     4741 ttactacacc catggttcac aggtcacggt ttcccatggt catctggctt ttttcggtgc
     4801 ttacgcactt ttgaatctca ctgtgtttta ctttgccatc ccgcggatca agggaattga
     4861 acagtacgat gcaagaaggg gcaaggtcgg gttttggaca atggtcacag cgatgacgct
     4921 catgggcctt tctttcggca tagcgggggt tgtgcaagct tatgttgaac gggtactcgg
     4981 aatgggctat atggccgcac aatcatacat gcggctctgg atggcagtca cgttcaccct
     5041 aggtctctgc ttcttcgtcg gcgttctgat aacggtaaca gacctcctca cattacgacc
     5101 cgcacgaaag gtgatcgatt aaagaggtaa tgagagcatg aaagacatct gagtagagtg
     5161 aggaggggaa gaaaagctgg aagggagata aattgcttaa aggcacattt ataagagaga
     5221 accaggtgag tcggacaacg ggatatcccc gggcaccaat tcaagatttt gtacgatgag
     5281 tgggtctgtc ccgcccaggg gcaccaaggg atgagactga aagaatatgc tgataaaata
     5341 accatcgact gcccaacggg aaggcttgag agcaaatagg aaatccttga ccagccttca
     5401 ccccgggaca acgcacagca aggtacaagg gaaggaggat ttacatggag tatgattttc
     5461 ttgtggatac ctatgatatc gagcggctta agaccctgaa cgtttggagc atgttcaggg
     5521 atgaggatct tgcgatccgc ccccatccac tgctcggaag agacagaaac ccccgtgagc
     5581 atatggttca tcagtgtgtg agtgagaata aatggttttg caacatgttc ggcattgatg
     5641 tcggtgcacc tcccttgccc gcgagagagg tgcggctcga attcataaaa cgatatgctg
     5701 aggattccgg aaagcggctg ctcattctca gggagaagga aaagacctgg tgggaacagg
     5761 aggccttttt ttttgatgtt aaacgaacaa ggacctggat aatggtgaga aggatagcac
     5821 atacggctca tcaccgggcg gagatgacga cactgctgag actactggga cggcaggttc
     5881 atagtgtgta tgggccttct gtcgataccg gtgggttacc ggacaacaaa gcactaacaa
     5941 tttacgccta tccggatata gactccctga ttgaaggcga atcaaggggc agttctaaga
     6001 aggccctgcc gggccccgga gacaaaccca gcacggaacg gcctgatttc taatcatgga
     6061 tgaggataac agtgggatgc gagcctaccc tctcttcaga aagcagggct ttcaccggag
     6121 atggcagccg caaaggaggc aaacgtcatg aaagggttat atttcataaa taccatggca
     6181 ttggtagccg tactgctctt gatgccggta gtgatgcacg gtcagtctgg gcagacagga
     6241 acagagaccc ctccgatagg gcagcctcta gctcgcgagg gtgacctggc cgttaaagtc
     6301 gtcgaggcac tgggattggg ctcgaccaac gacgaggtcg aggcagagag catggcgggc
     6361 tctgttggta ttgcaccgcg gaatggttgg atagctgatt atccggtgac ccctgatatt
     6421 atcggcgagg tacaggatac aatagtttac gccgctgact ccgggagaat atcaatcaca
     6481 agagatgatg ctttgaatgc ctaccggcag gttgtcgccg agatttccct accggtcaaa
     6541 tcatatacct atggagagac ctgtgaggct caagattgtc gaaattaccc caaccctacg
     6601 acaataagca actattattc tgatcaaggg cctccggttg tgacctacta tactccgccg
     6661 ccggattact attatctgta tggctgggtt ccctatccgt tctggtggtc tggtttctgg
     6721 ttcccggggt tcttcgtact ccacgatttc cacaaggtca tcatcgtgca taacagaccc
     6781 gtgttcgtta cgaaccactt cagccgtttc ggaactcccg gcgtcttcag agttgatcct
     6841 gtgcagaggt ttaacgggag gaccttcgcg gggataggag ctccccgtag cgggaggttc
     6901 ctctccaccg ggattgagcg ggcaccacat accgtcttca accctggccg acatcaaatg
     6961 atgcaaggcg gcggtaaaat gacgactcct tcttccggcg gcaggacagc ggcaccgtct
     7021 tctcgcagcg gtggaggttc ttcccaggga ggcgggatga ggaggtagac agctctcctc
     7081 ttccgcttca agcgtgacct gctgttgcat gagtacttta aaaagtgcgg aataagtcgt
     7141 gaaaagatta tgaggaaggt gggatagaat gctgatagaa aatgttctgt attttctgta
     7201 tattgagttt ttctctgctc taaatttgcc tgcaccccta aaataacaga ttgcagaaca
     7261 ggcgaaaaat ccattagaat caaacgcaag agtggcggaa ctggcagacg cgctggactt
     7321 taggacaccc tctgaacaaa tctgctaatc ccttgattat atctaactcc tcaatattac
     7381 tttatatttg aatgtcttga ttttctgcct attgcggtaa attctgtatt ttcagttccc
     7441 ttctgtgttc tgaaacctct tccatttgtt ctcaaagaat taggtatggt atccccagaa
     7501 gctaacagca tgtaattaaa ttgtgatatg ataagattgt gtattgagaa atatccatag
     7561 atgcatttca tgtcagagtc aaaggatgaa atgcattact taatcgataa tagcatccaa
     7621 ggttttcagc acgttcgatt gcacagtatt gaagctaaag catcagctga ttatcaaccg
     7681 tttttattat gcaccatcaa ggcacttccc atggataata gatgatcccg ccgaaggaat
     7741 ttatacgcaa gatccatccc ttttcttttt tatcagaaga tgatctggac atcatagtat
     7801 ctggattaga agtcgatcta ttcgaaaaag gcaagacaat ttataaaaaa gggcagacct
     7861 ctgaaaacgt ttatgtgata ttctctgggc tcgtcggcct ctttgatgat gaggcgccta
     7921 ttgattacct ctcccgaggt gagatcttcg gccttatgtc actttatgga aatctactga
     7981 atgcgactgc caaggctatg gaggatactg tttgctatgc agtggctcat cggcaattca
     8041 aaataatctt cgattcaaat gagaggtttg catcgttttt ctctactttg ctcaatagaa
     8101 gattacgctc gtttaaaacc atcgcttcgg ataagaagat tcttgaggag gccttattcg
     8161 ttcttgagct tgagaagata atatacaaaa atcctgttgt ttgcagccct gaaatgacga
     8221 tagctggtgc agcttttgag atggataaga attcggtaag ttcaatcgta attgtagaca
     8281 ccaatgcaat gccgatcggc atattgaccc ataaggacct gagaaaggtc ttaatcagcg
     8341 gtgatagatt agacccggtt acggagttta tgtcatctcc ggttaagaca atcaatgtgc
     8401 aggcgacaat attcgaagcc tattcaaaaa tgattgatgc cggaatagac cacctcgttg
     8461 tcactaagaa tgataaagtc tttggtataa tcacaaggaa ggacattcag atccaccttg
     8521 aaccctcttt ttcgatagtc aaactgttca gaaaggttca caaggcggcc tctatcgaag
     8581 agcttaaaac cgctttcgaa ggtctcatgg tatcagtctc gaagattgcg atgaccggtc
     8641 cgaatttctt cgatctgacc aggatgttat gctcagttca tgatgccgta gtgtacaagg
     8701 ttattcagat tctcatagat aaacatgccc ctgctcggtt cctgtggatt catatgggca
     8761 gttccggcag aaaggaagag attatagcta ctgatcagga caatgcctta atctacctag
     8821 gagataatcc tgcggcacta gctaaaaata tcaatgagtc actagccaaa attggctacc
     8881 ccacgtgctt cggaaactac atggcatcaa acagcttgtg gaatcagcct ctgccggtat
     8941 ggaaagaata cttcaaccag tggttttcag acccaattcc tgatcatctc cgatatcttt
     9001 ctgtgtttct ggacatgagg cctttatacg gagagacagc cttatacaat gaattgctgg
     9061 aatcgataaa agccgcagta aaagccgagg ctataaaact aatggctgag gataccattg
     9121 agatccgacc accacttggg atattcggaa tcctaggtct gcataagggc gtggatctga
     9181 agacctatgg tattttccct atagtaaacg gtgccagggt tctcgccgta gacagtggag
     9241 tttgggggat aaccaacacc aaggaaagat tggaggcgct gagtgccaat atgacgatca
     9301 gcaatgaaat gtgccacgat ctcatagagt cttatggctt tctgcaggat ttaaggctga
     9361 ggcatcacgc aagggcagtg ctgaataaat cccaggcaaa caacttgata actaccaagg
     9421 aactctcgaa tgttgatttg ctcatattaa aggagtcgct caagatcgtc tcagaatttc
     9481 agaagttctt gatgaaaaaa tatggggtta gtcgaaccgt catttatagt caattataga
     9541 taggaatatt tcgtcccatg gacctaaaag ct
//
LOCUS       DATKOU010000141         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4034686, whole genome shotgun sequence.
ACCESSION   DATKOU010000141 DATKOU010000000
VERSION     DATKOU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4034686"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..192)
                     /locus_tag="VMU21_06400"
     tRNA            complement(117..192)
                     /locus_tag="VMU21_06400"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(157..159),aa:Thr,seq:cgt)
     gene            387..677
                     /locus_tag="VMU21_06405"
     CDS             387..677
                     /locus_tag="VMU21_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77192.1"
                     /translation="MVKICASCEKEIEMDKYFSRKSTCPNCKRDLHICLNCRFYSETA
                     HNKCIEPKAEFQRARDRANFCDYFVYREGTSSLHSGNVQQDARKNFDDLFRK"
     gene            complement(723..1028)
                     /locus_tag="VMU21_06410"
     CDS             complement(723..1028)
                     /locus_tag="VMU21_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HUO77193.1"
                     /translation="MLIGFSVIPLGVGSSIGDQVAEVMKIIDASGLPYKVNPMGTVVE
                     GEWDEVMKLVKKCHRAVMKSAERVVTTISIDDRKGKPGRIEEKVKSVERRIGRSLKK"
     gene            complement(1171..1443)
                     /locus_tag="VMU21_06415"
     CDS             complement(1171..1443)
                     /locus_tag="VMU21_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HUO77194.1"
                     /translation="MTKAELIDKIASGAGLSKADASKALDSTLNAVKASLKKGQKVTL
                     VGFGTFSVVKRKARKGRNPRTGAVITIPAAKTPKFTSGKALKDAVR"
     gene            1673..3328
                     /gene="recN"
                     /locus_tag="VMU21_06420"
     CDS             1673..3328
                     /gene="recN"
                     /locus_tag="VMU21_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HUO77195.1"
                     /translation="MGIKDFAIIDTLTLRFEPGFNVLTGETGAGKSIIVDALGLTLGE
                     RAQTDMIKTGKNETTVEAFLDIPAHPFLERLGIPSNDGIILRRTLSSSGKSRAYINDT
                     MVNVQTLLDLGRSLVDIHGQHEHQSLLSPDNQRAVLDAYGKLTNARTEVAELFHEVQS
                     LTRELSLLKTDSREKQQRLDLLKFQINEIESASLEMGEVEKLREERAILANLAKLNEL
                     TDSAYLLLYGADGSSSEKLSLALSILREVSQIDHGMSEVLHLLESAMPLVEDAAGSLR
                     HYRDKYDMDPKRLAHIEDRLDFIKSLERKYGNGIEEILRYRDEGMQELQRLTLSDERI
                     IELGDELKEKEKGLHERAQQLSGMRREISKKIERAVKGVLKELAMEKAEFVIDVKPVP
                     LSSAGMDSVEFLLSANRGETPKALSRIASGGELSRIMLALKGILAEVDRIPVLIFDEV
                     DAGIGGRTADSIGMRLKTLGRRHQVVCITHLPQIAAMADHHLKVDKVTRKEGVYVKVN
                     ELSWEERVEEIARMLSGKVTEISLTHARELLGSEHIRKGTRER"
     gene            3318..3563
                     /locus_tag="VMU21_06425"
     CDS             3318..3563
                     /locus_tag="VMU21_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO77196.1"
                     /translation="MKGRITIDRERCKGCKYCVLACPKGVIVIDSEFNASGHFPAKTE
                     RMERCTGCALCAQMCPEIAIQVWVEEKDNSLSTSSQS"
BASE COUNT          997 a          883 c          902 g          825 t
ORIGIN      
        1 cttcgacgac gagacctgtc ggctcgagcc gatcgagaat ccgttcagtc cgaaagtgtt
       61 acctatgtct ccggaatgat ctgtcaccca tgtccccgga atggaccttg aagaaatgga
      121 gccggcggtc ggactcgaac cgacgacctg ctgattacga atcagctgct ctaccaactg
      181 agctatcccg gcattgggca tactagttta cattcttttc ccatgaaagc tcaatatcca
      241 ttgcggcagg ggaacctctg cactatgtat ccctcagaag acggtatggt acagtagaga
      301 aatgtccgag aggcaccggt gactgagaga tagtgctaaa ataggagggg actccagaag
      361 agggatgaag ggtagttagg gaggttgtgg tgaagatatg cgcatcatgt gagaaagaga
      421 tagagatgga taagtacttt tcgaggaaat ctacatgccc caattgcaaa agagaccttc
      481 atatctgtct caactgcaga ttctactctg agacagctca caataaatgt attgaaccaa
      541 aggcagaatt ccagagggca agagacaggg caaacttctg cgactatttt gtttacaggg
      601 aagggacctc ttctctgcat tcaggaaatg tgcagcagga tgcgagaaag aacttcgacg
      661 accttttcag aaaataatac cgtcaccccg aaccagctcc aggtcctcac ctcaagaggc
      721 cactactttt tgagtgacct tcctattctc ctttcgactg atttcacctt ttcttcaatc
      781 cttcccggtt tccccttcct gtcatcaatt gagatggtgg tgaccaccct ctctgcggac
      841 ttcatcacag cgcgatggca cttcttcaca agtttcatca cctcgtccca ttccccttcc
      901 accacagtgc ccatgggatt cactttatag ggaagcccgc tcgcatcaat aattttcatt
      961 acctcggcaa cctgatcacc gatactgctg cccacaccca atggaatgac actgaacccg
     1021 ataagcatgg gttcctcctt tccccaatca gggagacacg cgtattacgt tctaaaaaaa
     1081 acaagactat aaccacagga tagcacgaaa aaaccccaaa aaaaaaggga tagtgaatac
     1141 tatcccctga gcatgtaaaa ataagaaaaa ttatcttact gcatccttga gcgccttacc
     1201 tgatgtgaat tttggcgttt ttgcggcagg aatggttatt actgcacctg ttctgggatt
     1261 gcgccccttt cgcgccttcc tcttaactac cgaaaaggtc ccaaatccca ccaatgtcac
     1321 tttctggcct tttttcaatg atgccttcac ggcattgagc gttgaatcca atgcctttga
     1381 tgcatcagcc ttgctgagcc cggcaccaga agctatcttg tcgataagct ccgccttagt
     1441 catctaattc ccccctttct atggtatttc ttaggattcc gtgttcaata aaggtatcaa
     1501 gaagaactcc ttttgtcaag cccaaaatta agcatgaaat taagaaagaa tcgtgccaga
     1561 cagggagcct caaatttgtc ctgtctcttt gtatcttggg cagcagttga acaccgggaa
     1621 attggaattt ccctccccct ctatggtatt tttaggagtt atgctgcgcc aattgggaat
     1681 caaagacttc gctattatcg ataccctcac cctacgtttt gagcccggat tcaatgtcct
     1741 caccggggag accggggcag ggaagtccat tatcgtggat gccctcggcc ttacccttgg
     1801 ggagagggcc cagaccgata tgataaagac aggaaagaat gagaccaccg tagaggcatt
     1861 cctggatatt cccgcacatc ccttcctgga aagacttggg attcccagca atgacggcat
     1921 catcctcaga agaaccctct catcctcagg aaaaagcagg gcctatatca atgacaccat
     1981 ggtgaatgtg cagacgttgc tagacctcgg gcggtccctt gtggacatcc atggacagca
     2041 cgaacaccag agcctgcttt cgcctgataa ccagagggct gtcctcgacg cgtacgggaa
     2101 actcaccaat gcgagaacag aggtggcaga actctttcac gaggtgcagt ccctcacacg
     2161 ggagctttcc ctccttaaaa cggacagcag ggaaaagcag cagagactcg accttctgaa
     2221 gttccagatc aacgagatcg aatctgcatc cctagagatg ggtgaagtag agaaattgag
     2281 ggaagagagg gctattctcg caaatcttgc caaactgaat gaacttacgg attctgcgta
     2341 tctgctcctt tatggcgctg acgggtcttc ctcagaaaag ctttccttag ccctgtcaat
     2401 actgagggag gtatcccaga tcgatcacgg aatgtctgag gtccttcatc tcctggaatc
     2461 ggcaatgcct ctggtcgagg atgcagcggg ctcgctgaga cattacagag acaaatacga
     2521 tatggatccg aaaaggctgg cgcacataga agacaggctg gactttataa aaagcctcga
     2581 aaggaaatac ggtaatggga tagaagaaat tttgagatac agggacgaag ggatgcaaga
     2641 gctccagcgg cttaccctat cggatgagag gataatagaa ctaggcgatg aactgaaaga
     2701 gaaagagaaa ggactccatg aacgggctca acaactttca ggtatgagga gggagatctc
     2761 aaagaaaata gagcgtgcag tgaagggagt acttaaggaa cttgccatgg agaaggcaga
     2821 gttcgtgata gatgtgaaac ctgttccact atcgtccgcc gggatggata gtgtggaatt
     2881 tctcctttct gccaacaggg gagaaacccc aaaggccctc tcgcgaattg cgtcaggcgg
     2941 tgaactctcc aggatcatgc tcgcgctgaa ggggatcctc gctgaggtgg acaggatccc
     3001 tgtcctgatt tttgatgaag tggatgcggg cattggcgga agaacagcag acagcatcgg
     3061 gatgagactc aaaaccctcg gcaggagaca tcaggttgtt tgcatcaccc acctgcccca
     3121 gattgccgcc atggctgacc atcaccttaa ggtggataag gtaacgagaa aagagggcgt
     3181 ctatgttaaa gtaaatgaac tctcatggga agaaagggtg gaagagattg cacgcatgct
     3241 cagcggaaag gtgacagaga tttccctcac ccatgcaagg gaactgctgg ggagtgagca
     3301 tatcaggaaa ggcacccgtg aaaggtagaa tcaccatcga cagggagcgc tgcaagggat
     3361 gcaagtactg cgtgctcgca tgccctaaag gtgttatcgt cattgacagt gagttcaatg
     3421 ctagcggaca ttttcccgca aagacggagc gtatggaacg gtgtacaggg tgcgcgctct
     3481 gcgcacagat gtgcccagag attgccatac aggtatgggt ggaagagaag gacaactccc
     3541 tgagcacttc ctcacaatcc tgagtccgga agatgaagaa ccctgaggca agtccgcagg
     3601 gtatctt
//
LOCUS       DATKOU010000142        21163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3651369, whole genome shotgun sequence.
ACCESSION   DATKOU010000142 DATKOU010000000
VERSION     DATKOU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 21163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21163
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3651369"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2073)
                     /locus_tag="VMU21_06430"
     CDS             complement(139..2073)
                     /locus_tag="VMU21_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUO77197.1"
                     /translation="MRKDARGASVNGSRPGIRGTLQWSSGKQSMRMRWTIFLWMLIIL
                     SVDNGWADEKIKVGEVVVTASRFEESAQETTSEVTVLKAEDIKKMNVVLIPDVLRKVP
                     DLNVVQTGGDGRLTQVFLRGGDPKETLVMVDGVKVNSISTGGFDFSKVSVDDIERIEI
                     VKGSQSTIYGSEAMAGVINIITKKGEGKPRIVASFEGGSYGTYSPSLSISGSEGILSY
                     RVTGLYFYTQGISAAKDGAETDSYKNGYMSWNLGVKTSANSEVELFGNYSSDRTGLDD
                     FDFVTGRAIDSLTFVQHGYHYLAGVRGKLYLFDKWEQVLTVSGFGDLLKFADPAVQFN
                     NANVFDKRQGVDWQNNLHLSEVLMLTAGLAYQHESTNSLGNFDEGLNDKAGYLNSRLK
                     LFQDTLILNAGLRYDDYSTVGSKTTYRVGAAYMNKEVDATVRVSYATGFRVPSLNDLF
                     FPFYGNPSLKPEESKSFEAGIVKPLFQGKIQLGISYFNADYTNLIQADPLTFTAVNIA
                     NATIKGVEATASFQATDALSLKAGYTYLDAKDTDTHLPLNWRPANKVVLGIAYSTPAY
                     SLLADYNYVSGRFNSSHDAAIGKRLDPYWLVNLSGTYRLSKVISLFARIENLFDADYE
                     EVFSYGTKGLSSYGGMRVTF"
     regulatory      complement(2099..2284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2348..3046)
                     /locus_tag="VMU21_06435"
     CDS             complement(2348..3046)
                     /locus_tag="VMU21_06435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUO77198.1"
                     /translation="MVTTLYLIRHGETEGSALKRYLGSMDIPLSEKGVQQVRDVSAFI
                     REDLKTLGVAQHSSYLNEVHRAPHTGMESGVKEGTLQAVYSSNLSRAIKSGQIVAEPY
                     ALQIIVVPELRERSFGIWEGMTFSEIKETYPSEFESWAGNPLKYSPPGGENTMEVHER
                     VIRALEMILSNHGGDRIAIVAHGGVNRIILCHLLGIPLENIFRIEQNHGAVNIIEFWE
                     KYPVVKLMNRVIHE"
     gene            complement(3025..3837)
                     /gene="cobS"
                     /locus_tag="VMU21_06440"
     CDS             complement(3025..3837)
                     /gene="cobS"
                     /locus_tag="VMU21_06440"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HUO77199.1"
                     /translation="MRPLILAFQFLTIIPLKVSGGVSERDLSRSTVFFPVVGAFQGLL
                     AAVVARLSLTLLSGDAASALAITALVLCNGGFHLDGLADTFDALAVKSRGDERIDRDR
                     RLAVMKDSTTGAIGVVSIVLAILLKFVFMRELLTRASVDAASSFLFLMPVFSKWVMVP
                     SLYIGTSARQDGLGKAFMDSVTVSTVAYSSLFLLLFCAMASSLHLYKIFGAGTLALFF
                     SLCATFAVFSFMTVRFCTKRFGGLTGDIFGAINEISEILFLFVVVLWLQRSI"
     gene            complement(3838..4893)
                     /gene="cobT"
                     /locus_tag="VMU21_06445"
     CDS             complement(3838..4893)
                     /gene="cobT"
                     /locus_tag="VMU21_06445"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HUO77200.1"
                     /translation="MLLERLKEIRPVRADFAEIAQEHLDHLTKPKGSLGRLEEFARRL
                     VAITENKSPALDKKVIFTFAGDHGVAEEGVSAYPKEVTPQMVLNFLNGGAGINVLARH
                     AGSEVVVVDIGVDFDFGAIDGLVSRKVMRGTNNMRKGPAMTRQEAEKCLEVGIELSEV
                     FAKKGYKIFATGEMGIANTTPSAAIAAVLTGRAVSEVTGRGTGIADDVLKNKIMVIED
                     SIALNNPDPKDPIDVLAKIGGAEIGGIAGLIIGAAAQRIPVIVDGFISTAGALIAYSL
                     EPLTREYMFAAHNSVEIGHRVMLEKLGLRPILDLDMRLGEGTGAALAMLMIEAGLKIY
                     REMATFGDAGVSTERVR"
     gene            complement(4904..5422)
                     /gene="cobU"
                     /locus_tag="VMU21_06450"
     CDS             complement(4904..5422)
                     /gene="cobU"
                     /locus_tag="VMU21_06450"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HUO77201.1"
                     /translation="MKKKIVFITGGARSGKSRFALEEALRVSGRKAFIATAEESDDEM
                     RERINRHKQDRGEGWETYEEPLKIADVLGEMEGDYGVVVIDCLTLWLANVMQSALDVD
                     AEIEQLISSLARLSLPSVFIVSNEVGMGIVPENELARRFRNVAGRLNQRVAGAADEVY
                     VTLSGIPVKIKG"
     gene            complement(5444..6220)
                     /locus_tag="VMU21_06455"
     CDS             complement(5444..6220)
                     /locus_tag="VMU21_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO77202.1"
                     /translation="MSLVSNNTILDLQEVSFSYGEPAPFIRNISFSVREGEFIGLLGA
                     NGSGKSTILKLGSGILKPALGELTLWGKPIRTYKNKDRAKLICYLPQTLDITVPFTVR
                     ELVSMGLYPYDVPPSMTAEEALEMVGLGDRADAHLSDLSGGERRRTFIAMTLVQGAGL
                     LLLDEPLANLDIRYQIELIRLLRRLREERNISILMALHDINIALQFEKVMLIKNGEIL
                     GLGDSETVLTDAMLKESFDVNIEVRRSVDGRAHIAYENNF"
     gene            complement(6204..7166)
                     /locus_tag="VMU21_06460"
     CDS             complement(6204..7166)
                     /locus_tag="VMU21_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUO77203.1"
                     /translation="MRIRMFLIIALFFLVLVLTPFLGPTVISPFAVDATSREILFSIR
                     APRVVVAALMGMALGASGAVLQGILRNPLADPYILGISSGASLCAVLGILGGLTFLGE
                     FTVPVLAFIGAIVTGSIVGIMGWKRGGLWPERLLLAGVGLSFLFSALLMLIMSVSSDE
                     GMRKAILWIFGDLSMSEWARIPYGLAFVLAGIILSGTKAKALNALILGDEFAHSLGFT
                     PHRERFILFVAVGLLTAASVSLGGMIGFIGLLVPHIIRFFMGSDGRVLIPASALVGGA
                     LLCVADLISRSVLPPMELPAGIITALIGSPYFLYLLRRKNVLGV"
     gene            complement(7168..8019)
                     /locus_tag="VMU21_06465"
     CDS             complement(7168..8019)
                     /locus_tag="VMU21_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HUO77204.1"
                     /translation="MEKIFLFLMLIVTCSFSSSAHAGETPQRIISLAPNMTEILFALG
                     LGNRIVGVTSFCDYPAEAKRKPKVGGMSNPSLEAVISLKPDLVVMTIDGNPREFEERL
                     RSLGIKTYVFRASRLRELPKGIRDLGKALGVQEEAETFAREFEAAVKRVGAGEGMTGS
                     HTLSARKRVLFIVWPEPLIAAGPGTVIDDALALIGFDNIASDSRVAYPKYSIEEIIRR
                     APDVIIIGRGMERQDIEKISAGLLKRLHSVPAVRNKRVFYVSDDLYRLGPRTIRGIDE
                     ISRLVGQ"
     regulatory      complement(8229..8413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8499..8831
                     /locus_tag="VMU21_06470"
     CDS             8499..8831
                     /locus_tag="VMU21_06470"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUO77205.1"
                     /translation="MKKDRTLYELQSDVCKTLASPKRIEILNALKEGEKTVSELVEIL
                     GVPKANVSQHLAVMRHKGILKFRREGINMYYSVSNRKVIEACLLMKEVLMEQMKEKGK
                     LAEMASKS"
     gene            complement(8976..10496)
                     /gene="cysS"
                     /locus_tag="VMU21_06475"
     CDS             complement(8976..10496)
                     /gene="cysS"
                     /locus_tag="VMU21_06475"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HUO77206.1"
                     /translation="MEKHVAELQKRIEQLEGKGGMMRVYNTLTGKKEDFNPLTPGRVG
                     IYACGVTVYDLCHIGHARSAIVFDVMRRYLAFRGLQVNYIRNFTDIDDKIINRAKQEG
                     ISWDAVARKYTGEYYRDMDRLGVGRADVEPKATEHMAEIIGIVKGLMDRGFAYAVDGD
                     VYFEVNKFPGYGKLSKRDTEDMLAGARVEVDERKRNPMDFALWKSSKEGEPSWDSPWG
                     PGRPGWHIECTAMSIKHLGESFDIHGGGADLIFPHHENELAQSEAYTGKPFVRYWIHN
                     GFITIDREKMSKSLGNFFTIREILDKFDAEVVRFFLLSTHYRSPIEFSDELLREAEVS
                     IDRYYTTATRMEDFLKGGVGTQKPSAGDHALEETLAVFMDRFREAMDDDFNTAQALGH
                     IFEVIREVNRFLDNHPAGQKANDLVLRAKDLLTQAGKVLNIFNRTPEEWYDALMLTKR
                     IPYTKTDILQKIQQRKDARQRKDWAAADAIRKELEEQGIILEDKKDGSTWKVRVPR"
     gene            complement(10470..11099)
                     /gene="cysE"
                     /locus_tag="VMU21_06480"
                     /pseudo
     CDS             complement(10470..11099)
                     /gene="cysE"
                     /locus_tag="VMU21_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            11355..11747
                     /locus_tag="VMU21_06485"
     CDS             11355..11747
                     /locus_tag="VMU21_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO77207.1"
                     /translation="MSDDIKTMIADYMEKGFLENIIDMFKHDKGLYPLIGELMTDERM
                     RVRLGISALVETLAKEDRNTIARAIPGIAHLLRNENPIIRGDAAYLLGIIGQREVLPF
                     LVEAADDNNSGVRQIVREAIEEIGETEG"
     gene            11939..14578
                     /locus_tag="VMU21_06490"
     CDS             11939..14578
                     /locus_tag="VMU21_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUO77208.1"
                     /translation="MTNNTIISLFLNAAGRYPDNTAFNYFAQSWRTITYGEFLGKTRG
                     IASHLIQEGVRKGDRIAIILENRHEWCASYLGVLIAGGIAVPMDAQLGSNEVKNLLND
                     SGAALVVHSDRTETNVKSQIRGINVDSTEFQEICRTPFRGHYPNLTEEDLASIVYTSG
                     TTGTPKGVMLTHKNFCSDSEALISAGIVTHEDNVLSILPLHHTYAFMCTFLVPLFLGA
                     TITYPLGMKGPELISTMKERGVTVLVAVPQLLELIRNGIMKKFREMPSPVSLPLLLLL
                     RISGGLRKSTDLNMGRVIFASAQRAFGERFRFFASGGARLDPMVKDDLEALGFTALEG
                     YGLTETSPVVTFNPVEKRKSGSAGRPLPGAEIKILNPSGTGEGEIAIRGPMVMKGYYK
                     HPEATAQVIRDGWFLSGDLGYLDNEGYLFITGRAKEVIVLSSGKNVYPEEVEKAYQEI
                     PLVKEICVTGIEKEGIVESLHGVIVPNLDHARKERIGNISDSLKWAMNKVALKLPPYM
                     RLKGFTLSSEPLPRTPLGKLRRYMVRDIISKARGGRREAKGEDSLLLRDETGRVVEGC
                     ITPLLREAMPVHLSDNLELDLGLDSLQRIELVVALEKAFSFKLPETFASEVQTVGELV
                     ERIKEFKTRGISGAEKPLWEDIFAAEIPEEERKKIGLYQGRVEWAFSAVCIKLIKFFL
                     RVFFRLEARGIENIPAFPFIIAPNHCSNMDGFVVGLSIPLAVFKSLYFQGYQTYFQGW
                     LPSLFARLAHVIPIDPETFLSKALQLSSYVLKKNRALCIFPEGGRSIDGSLLEFKKGI
                     GILAMEHNIPVVPVLIEGTFEALPRGALWPKFVKITLTIGRPLYAAKVDFSKKPEGID
                     EYQFFANEVRERVKGLKEFPADQ"
     gene            15013..15960
                     /locus_tag="VMU21_06495"
     CDS             15013..15960
                     /locus_tag="VMU21_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HUO77209.1"
                     /translation="MLKQFTDYVTFTLLELEPTSHAGEALNFFIYDTLKIFTLLTTIV
                     FVVAIIRAYFPPEKTKRILAHEREYIGNVMAALLGIVTPFCSCSAVPLFIGFVEAGIP
                     LGVTFSFLISSPMVNEIALIMLWGLFGWKIALIYTSTGVLLAIASGIVIGRLKMEKHV
                     QDYVWEMKVGNSEIVETSFGQKIDYARNYTAELLKKIWPYVIIGIGLGGIVHGYIPTD
                     FVVKYAGPDNPFAVLIAVLIGIPLYSNAAGMIPVVQALVGKGMAMGTVLAFMMSVTAI
                     SFPEAVILKNVLKMRLIYIFFGIVAVGIIIVGYLFNTIL"
     gene            15972..16211
                     /locus_tag="VMU21_06500"
     CDS             15972..16211
                     /locus_tag="VMU21_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUO77210.1"
                     /translation="MEIKVLGPGCANCHKMEELAKTAVKELGVEARVEKVSDIQEIMK
                     YTMSTPGLVVNGKLKHSGKPLPNIEKVKTLIKEEA"
     gene            16214..16588
                     /locus_tag="VMU21_06505"
     CDS             16214..16588
                     /locus_tag="VMU21_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUO77211.1"
                     /translation="MQDLMNIFKALSEETRLRILKLLEHGELCVCDIVAALELIQPKV
                     SFHLNVLKEAGFLKDRKQGRWTHYRIDDSDFFRRFLLMSVLERIHNKAVSEDNKRLDA
                     FMQTKKTKATVVDIEKRRGCCG"
     gene            16648..17076
                     /locus_tag="VMU21_06510"
     CDS             16648..17076
                     /locus_tag="VMU21_06510"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HUO77212.1"
                     /translation="MDKKKVLFVCVHNSARSQMAEAFLNAMGGDKVVAESAGLEPGVL
                     NPIVVDVMKEVGFDISNNKTQGAFDCFKAGKLYHYVITVCDETNAERCPIFPGLAKRL
                     HWGFDDPSKLQGSREEKLQGTRIIRDQIKAKREEWISQQQ"
     gene            17102..18181
                     /gene="arsB"
                     /locus_tag="VMU21_06515"
     CDS             17102..18181
                     /gene="arsB"
                     /locus_tag="VMU21_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HUO77213.1"
                     /translation="MNPTITRRLSFLDRYLTLWIFSAMAAGVGLGYFIPQTESLINSF
                     QVGTTNIPIAIGLILMMYPPLAKVKYEELGEVFRNKKVLTLSLVQNWVIGPILMFLLA
                     IIFLRSYPEYMVGLIMIGLARCIAMVIVWNDLACGDPEYAAGLVAFNSIFQVLFFSVY
                     AYVFITVLPSWVGLKGVVVNVTIGQIAKSVFIYLGIPFLAGMITRFSLLKIKNREWYE
                     KVFIPRISPITLIFLLFTILVMFSLKGNYIVRLPLDVIRIAIPLLIYFVFMFLVSFYL
                     GKKIGADYPKTATLSFTAASNNFELAIAVAVAVFGLNSGAAFAAVIGPLVEVPVLISL
                     VNVALYFQKRYFTIEYCRVKGVESP"
     gene            18218..18598
                     /locus_tag="VMU21_06520"
     CDS             18218..18598
                     /locus_tag="VMU21_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUO77214.1"
                     /translation="MKKGLMLLVALITLLTLFEYSNASDVDSMLSNARKDGKAVMLEI
                     GSEGCVPCEQMKPVMEKLRTNYKGKLEVFFVDVRKDKETSRKFGVHVIPTQVFLDKNG
                     KEFHRHFGFYEYEEIVPVLKKAGL"
     gene            18631..19347
                     /locus_tag="VMU21_06525"
     CDS             18631..19347
                     /locus_tag="VMU21_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUO77215.1"
                     /translation="MKIAILGDIHGNLEALEVAYTTAVSEHVEKIYHLGDIGGYAPFV
                     NEVVDFLIEHGIAGIQGNYDETVANDREHCGCKYEDPIQEEMANRSFEWTKRHASQKT
                     KEYLKKLPFSISFQAEGKKVLLFHATPGKNNLYWYEDRPEKFFREMARKVDADVMIYG
                     HTHKPYRMDMDNKVFINAGSVGKPKDGDPRACVALIEITKGAVVTNFLRVPYDIAKTT
                     EAIVASGLPAYFADKLKEGR"
     gene            19349..20041
                     /locus_tag="VMU21_06530"
     CDS             19349..20041
                     /locus_tag="VMU21_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HUO77216.1"
                     /translation="MLESLLGNIQTVIQNRHGLAFVAVFIGGIISAASPCVLAAIPLI
                     IGYVGGYSEGNKRRAALYSLVFILGLSLTFTLLGAAASIMGQFLGFMGKWLYLGLALI
                     AVLMGLQLMGIIEIPLPFQKTRAVKTKGLLGAFLLGMLTGTVSSPCATPVLAVILAYV
                     STQGDMIYGGSLLFVYALGHCALIFMAGLSVGVTESIVRSRGIRNFSLYSKRFSGAIL
                     VAVGIYFGITNI"
     gene            20178..20720
                     /locus_tag="VMU21_06535"
     CDS             20178..20720
                     /locus_tag="VMU21_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUO77217.1"
                     /translation="MMLLSGYMKDVSLPVCNSSFQSLHCIAYLNEDITAVLPYLNAEL
                     GGHQYIKEPPSVTFKINGRLITIHPTRIAVNALESVEQADKILEWLRKQINDIWERRE
                     EIEPSYKRVASPTLLDILKLLPRTNCKKCGQSTCLVFATQIMEGGKGPEHCPALNAVE
                     RRKLEHYLNQFSITGGVMSK"
BASE COUNT         5287 a         5305 c         5188 g         5383 t
ORIGIN      
        1 cacgttgttc cggagaaaga tagctgaaca taaagccctg tgggtcgttg atgcctctca
       61 tcatgcctcc tgcgatgatg tcgttcatca actatacctt atgcgtatat acattggaag
      121 tagtttttca gcagcctgct aaaacgtcac tctcattcct ccgtaggaag ataacccctt
      181 tgttccgtaa ctgaacactt cctcataatc tgcatcaaag aggttctcga tccgtgcaaa
      241 aagagagatg acttttgata gtctataggt tccgctcagg ttcacgagcc agtagggatc
      301 aagtctcttc cctatcgccg cgtcatggga agagttgaac ctcccgctga cataattata
      361 atctgcaaga agggaatagg ccggcgtaga atacgcaatg cctaagacca ctttatttgc
      421 cggcctccag ttgagcggta aatgcgtgtc tgtgtccttc gcgtccagat aggtataccc
      481 ggccttcagg gagagtgcat cggttgcctg gaacgaggcg gtggcctcaa ctcctttgat
      541 ggtagcgttt gcgatgttga ccgcagtgaa ggtgagggga tcggcctgta tcagatttgt
      601 gtaatcggcg ttgaaatagc tgatgcccag ttggattttt ccctgaaaaa ggggcttgac
      661 gattcccgct tcaaaggact ttgattcttc cggtttgagc gagggatttc cgtagaaggg
      721 gaaaaagaga tcattcagag acggcacccg gaaaccggtc gcgtagctta cccttactgt
      781 tgcgtcaacc tccttgttca tgtacgcggc accaaccctg tatgtggtct tgctgcccac
      841 tgtactgtag tcgtcatacc tcagaccggc attgaggata agagtgtcct gaaagagctt
      901 tagtctgcta ttgaggtatc ctgccttatc atttaggcct tcatcaaaat tgccgagact
      961 gttcgtgctt tcgtgctgat acgcgaggcc ggcagtgagc atcaggacct cggagagatg
     1021 gagattgttc tgccagtcca caccctgtct cttgtcgaat acattggcat tgttgaattg
     1081 cactgcgggg tcagcgaatt taagtaaatc accaaacccc gagactgtca atacctgctc
     1141 ccacttgtcg aaaaggtaga gcttgcctct caccccagcc agatagtgat agccatgctg
     1201 aacaaatgtt aaagagtcga tggccctgcc ggtgacgaaa tcaaagtcat cgagaccagt
     1261 acggtcagag gagtaattcc cgaagagttc gacctccgaa tttgctgatg tcttgactcc
     1321 gagattccac gacatgtagc cgttcttata actgtccgtc tcggcaccgt cctttgctgc
     1381 tgatatgccc tgggtataaa aatacagacc ggtcacccgg tagctcagta tcccttcgct
     1441 ccctgaaatg ctgagcgagg gagagtaggt gccgtatgag ccgccttcaa acgacgcgac
     1501 gatcctcggt ttcccctcac ctttttttgt gatgatgttg attactcctg ccattgcctc
     1561 tgagccgtat atcgtgctct gggagccctt gacgatctct atccgctcta tatcatcaac
     1621 agagaccttc gagaaatcaa agccgccggt ggagatgctg tttaccttaa ccccgtccac
     1681 catgacgagc gtctccttgg ggtctccgcc ccgcaagaag acctgcgtaa gcctcccgtc
     1741 tcctccggtc tgcacgacat tcagatccgg aacctttctt aatacatcgg gaattaagac
     1801 aacgttcatc tttttgatat cttcggcctt caggacagtg acctcgcttg tcgtctcttg
     1861 cgctgactct tcaaacctcg aagcagtgac caccacttcc cccaccttta ttttctcatc
     1921 cgcccaccca ttgtccacgg atagaattat gagcatccac agaaagatcg tccaccgcat
     1981 cctcatcgat tgttttcccg agctccattg aagtgtgcct ctgatgccgg gcctgctgcc
     2041 attgacagaa gccccccttg cgtcctttct cattacagac ctcctcttcc ctcgaagggt
     2101 attcgtcttc aggcattgag ggtctcctga ctcagggcaa cctacttgcc ggccttcccg
     2161 tcccatctat caggacagtg gcgttgcggc gttcgttccc ctaacagttg cggggcagtt
     2221 cccggctttc acgggattcc cctcacctgc ctgtatggga ctgtcaatga cagtcgtgca
     2281 taatctttgc aatgtcttcc ttccacctga gccatgttct gaaccttcat cgatggaatt
     2341 gagcgcatta ctcgtgtatc actctgttca tcagttttac gaccggatat ttctcccaga
     2401 actcgatgat gttcactgct ccgtgattct gttctatcct gaagatattt tccagaggga
     2461 ttccgaggag gtgacagaga atgatcctgt tcactccgcc atgagcaact atggcgatcc
     2521 tgtctccccc atggttgctc aaaatcattt ctaatgcccg gatgaccctc tcgtgcacct
     2581 ccatggtgtt ctctcctccc ggagggctgt atttcaaggg gttaccagcc catgattcaa
     2641 attcagaagg atatgtttcc tttatttccg aaaaggtcat tccctcccat attccgaagc
     2701 tcctctccct gagttcaggg acgacaatga tctgtaatgc atagggctcg gcgactatct
     2761 gcccgctctt tatagccctg ctaagattgg aggagtatac tgcctgaagt gttccttcct
     2821 tcacaccgga ttccataccg gtatgaggag cgcggtggac ctcatttaga taactcgaat
     2881 gttgcgcaac acccaaggtc ttcagatcct cgcggataaa agcggacacg tccctgacct
     2941 gttgaactcc cttttccgaa agggggatgt ccatgctccc gagatagcgt ttgagcgcgc
     3001 ttccctctgt ctcaccatgc ctgattagat agagcgttgt aaccatagca ctaccacgaa
     3061 caaaaagagt atttccgata tctcattgat ggcgccgaat atatctcccg tgagtccgcc
     3121 gaaccgtttc gtgcaaaatc gcactgtcat gaaactgaag acagcgaatg tggcacagag
     3181 agaaaaaaag agtgcaagag tgcctgcgcc aaatatctta tagaggtgca gtgaagaggc
     3241 catagcgcag aagaggagca ggaagaggga tgagtacgca actgtgctta ctgtgacgga
     3301 atccataaaa gcctttccca gtccatcctg acgggcggat gtcccgatat aaagggaagg
     3361 gaccatgacc cacttcgaaa ataccggcat gaggaacagg aaagacgagg cagcatcgac
     3421 cgaagccctt gtgaggagct cacgcatgaa aacgaatttc aaaaggatgg caagtactat
     3481 ggaaacgact ccgatagcac ctgtagtgct gtccttcatt acagcgagcc gtctgtccct
     3541 gtcaatccgt tcatccccgc gtgatttcac tgccagtgcg tcgaacgtgt ccgcaagccc
     3601 gtcaaggtga aatcccccgt tgcaaagtac cagtgctgta attgccaggg cgcttgcggc
     3661 gtccccggaa aggagcgtca aggacaaccg cgcaacgacg gccgcaagta ggccctgaaa
     3721 tgcgccgacc acagggaaaa agaccgttga tcgggaaaga tctctctcgg agacccctcc
     3781 gctgaccttt aacggtataa tggtaaggaa ctggaatgcg aggatcaatg gcctcactta
     3841 cctcaccctc tctgtcgaaa cccctgcgtc accaaaggtc gccatctccc ggtatatctt
     3901 gagtccagcc tcgatcataa gcattgcgag cgcggccccg gtaccttcac ccagtctcat
     3961 gtcaaggtcg aggataggcc ttaaacccaa tttctcgagc atcacccggt ggccgatctc
     4021 aacggagttg tgggctgcga acatgtattc tcttgtgagc ggttcgaggg agtacgcgat
     4081 caaagctcct gcagtggaaa taaagccgtc aacgattaca gggattcttt gtgcagccgc
     4141 gccgataatc aatcccgcaa tccccccgat ttcagcgcct ccgatcttcg cgaggacatc
     4201 aatggggtct ttaggatcag gattgtttaa tgcaatggaa tcctctatca ccatgatttt
     4261 gttcttcagg acatcgtcag cgattccggt gcctctcccg gtgacctctg aaaccgccct
     4321 gccggtcagg actgctgcga tggcagcaga aggggttgtg ttcgctatcc ccatttcccc
     4381 ggtcgcgaag attttgtacc ccttctttgc aaagacctct gaaagctcta tccccacctc
     4441 caagcatttt tcagcctctt gccttgtcat ggcaggccct ttacgcatgt tgtttgtgcc
     4501 cctcatgact ttccgggaga ccagtccgtc aatagctccg aaatcaaaat cgactccgat
     4561 gtcgaccacc acgacctctg agccggcatg ccgggcgagc acatttattc ctgccccgcc
     4621 attcaggaaa ttcaagacca tttggggggt aacttccttg gggtatgcag aaaccccttc
     4681 ctcagcaacc ccatggtcac ccgcaaaggt aaagattacc ttcttatcga gtgccgggct
     4741 cttgttttcc gtaatcgcaa ccagccttct cgcaaactct tcgagtctgc cgagactccc
     4801 ctttggtttc gtaagatggt cgaggtgttc ctgtgcgatc tctgcaaagt cagccctgac
     4861 aggtctgatt tcttttaatc tctcaagaag catagtctct cctttatcct tttattttta
     4921 ccggtatacc cgacaaggtc acatacactt catccgcggc tccggcaacc ctctgattca
     4981 atcttcccgc cacatttctg aatctccttg cgagctcatt ttcgggaaca atccccatgc
     5041 ccacttcatt agaaacgata aaaacactgg ggagactcag cctcgccagt gacgagatga
     5101 gctgttcgat ttccgcatct acatccagtg cagactgcat tacatttgcg agccaaagag
     5161 taagacaatc tatcacaacc accccgtaat ccccttccat ctccccaagg acatccgcga
     5221 tctttaatgg ctcttcataa gtctcccatc cttccccgcg gtcctgctta tgtctattga
     5281 tcctctctct catctcgtca tcactctctt ctgcggtggc gatgaatgcc ttccttcctg
     5341 aaacccgcaa agcctcttcg agtgcgaacc ggctttttcc gctccgtgcg cctccagtaa
     5401 taaagacaat tttcttcttc acaggcatcc ccctccgctc tccttaaaaa ttgttttcat
     5461 acgcgatgtg cgccctgccg tcaacagacc gtcttacctc aatattcacg tcaaacgact
     5521 ccttgagcat ggcatccgtg agaaccgtct ctgagtctcc caaaccgagt atctcgccgt
     5581 ttttgatgag cataaccttc tcaaactgaa gcgcgatgtt gatatcatga agcgccatga
     5641 gtatagagat gtttctttct tctctcagcc gtctgagaag gcgtatcagt tctatctggt
     5701 atcggatatc aaggtttgca agcggttcat caagtaggag gagacctgcg ccctgcacca
     5761 gggtcatggc gatgaacgtg cgccttcgct cacccccgct caggtcggaa agatgcgcat
     5821 ccgccctgtc tccgaggccg accatttcaa gtgcctcctc agcagtcatc gagggaggga
     5881 cgtcataagg ataaagaccc atgctcacca gctcccgcac tgtgaacggg acggtgatat
     5941 cgagggtctg cggcagatag cagatcaatt ttgcgcggtc cttattctta tatgtacgaa
     6001 taggtttccc ccaaagcgta agctctccta aagcaggctt gagtatcccg cttcccagtt
     6061 tcaaaatcgt agatttcccc gatccgtttg caccgaggag ccctataaat tctccttcgc
     6121 gtacagagaa ggaaatgttg cggatgaagg gcgcgggttc tccgtaggaa aacgagacct
     6181 cctgaagatc gagtatcgta ttgttagaca ccaaggacat tcttcctcct gagaagatag
     6241 agaaaatagg gtgatccgat aagagcagtg atgatgcctg cagggagttc cattggggga
     6301 agcacggacc tgctgatgag atcagcgacg cacaggagcg caccacccac gagtgcggaa
     6361 gccgggataa gaaccctccc gtcagacccc atgaagaatc tgattatatg gggaacgagc
     6421 agtcctataa agcctatcat tcccccaagc gatactgatg cggcggtcag gagacctacc
     6481 gcaacaaaga ggatgaaccg ctcccggtgg ggagtaaacc cgaggctgtg ggcgaattca
     6541 tcgcccagaa tcagcgcatt cagtgccttt gcctttgtac ctgacaggat gatcccggca
     6601 agtacaaagg cgagcccata gggtatccgg gcccactcgg acatggagag gtctccgaat
     6661 atccagagga tcgccttacg catcccctca tccgacgata cgctcatgat gagcatcagg
     6721 agggctgaaa aaaggaagct cagtcccaca cccgcaagga gaagcctctc cggccagagt
     6781 cctcccctct tccagcccat tatgcccaca atgcttcctg taacgatcgc tccgataaac
     6841 gcaaggaccg gtacggtaaa ctcgcccaag aaggtcagac cgccgaggat gccaaggact
     6901 gcgcagagcg atgcaccgct tgatatgcca agaatgtaag ggtctgcgag ggggtttctg
     6961 agtatcccct gcaataccgc ccccgaggcc ccgagcgcca ttcccataag tgccgcaacc
     7021 acgacccggg gcgccctgat ggaaaagagg atctccctcg acgtggcatc aacggcaaaa
     7081 gggcttatca ccgtaggtcc aaggaaaggg gtgagcacaa ggaccaagaa aaagagtgca
     7141 atgatgagga acatccttat tctcatatta ctggcccacc agtctcgaaa tttcatcaat
     7201 acccctaatc gtccttgggc cgaggcggta gaggtcatcg ctgacataaa agactctttt
     7261 gtttcttact gcagggacag aatgcagcct cttcagaaga cctgccgaga ttttttctat
     7321 gtcttgacgt tccattcccc tccctatgat gataacgtcc ggtgccctgc ggataatctc
     7381 ttcgatggaa tactttggat atgccacccg ggaatccgat gcaatgttgt cgaaaccgat
     7441 gagggccagc gcgtcatcga tcaccgtgcc tggacccgca gcgatcaggg gttccggcca
     7501 tacaatgaaa aggacccttt tccttgctga gagtgtatga gagccggtca ttccctcacc
     7561 ggctccaacc ctcttcaccg ctgcttcgaa ctccctcgcg aacgtctctg cctcttcctg
     7621 cacacccagt gcctttccca ggtctctgat gcccttaggc aattcccgga gtctgctcgc
     7681 tctgaagaca taggtcttga tcccgaggga gcgcagtctc tcctcaaatt ccctggggtt
     7741 tccgtcaatg gtcatgacca ccaaatcagg tttcagtgag atgaccgcct ctaatgacgg
     7801 gttggacata ccgccgacct tcggcttcct ctttgcctcg gcaggatagt cgcagaagct
     7861 tgtgactccc acgatcctgt tgccgagacc aagggcaaaa agtatctctg tcatattggg
     7921 ggcaagggaa atgattcgct ggggtgtttc ccctgcatgg gcagaggagg aaaacgaaca
     7981 ggtaacaatg agcattaaga acaaaaatat cttttccatt tcatttgcca tggacacagt
     8041 acaaaataaa aaaatcccct cgcctgcggc gagaggattg caccctgttt cacttcatcc
     8101 ccaaccggta tcccgaaatg tcccctgact tttcaggaac ccctcttcct ccacgggaag
     8161 gcatttcgtt catagtcatt ggctcattgc tcatggtgta tgaagctgaa aacctatgag
     8221 ccaagagcta tgagctatca tccacaggca ggtcttctgg ctcacggatc gtcctactca
     8281 ccggccttcc cgtcccgatt tgtcgggaca gtggcgtgtg tggttttcgt acccgtttac
     8341 agcggcggga ccgctcccga ttcttacggg attccctgtt aggcctttcg gcgcctgttg
     8401 cgctgactat taaaaatatc taaatgctac cacaaatgtc aagaagagcc ctccaataaa
     8461 ggcttgacct ttggacccaa aatgaataga atagccaaat gaaaaaagac aggacacttt
     8521 atgaattgca gtccgacgtt tgcaagaccc ttgcaagccc caagaggatc gagatactga
     8581 atgccctgaa agagggtgaa aagacggtga gtgagcttgt cgagatcctg ggggtaccca
     8641 aggcaaatgt ttcgcagcac cttgcggtca tgagacataa ggggatactg aaatttcgac
     8701 gtgagggtat caatatgtat tacagcgtct cgaaccgtaa ggtcatcgag gcatgccttc
     8761 tcatgaaaga ggtccttatg gaacagatga aggagaaggg caagctggcg gagatggcct
     8821 ccaagtcgta atcttctttt accctccgac atctctactg ttctgaacgc gctcctaaat
     8881 gctgtggcac accaggacat gcaagggtta tatgttgcgg caacccggct tttgcccgca
     8941 gagacaggga caaaggactt aagaacctcc cgcagttacc tgggtacccg caccttccag
     9001 gtggagccgt ctttcttgtc ctcaaggatt attccctgtt cttccagttc ttttcttatg
     9061 gcatcagccg ctgcccaatc cttcctctgt cttgcgtcct tcctctgctg aattttctgg
     9121 agaatgtccg tctttgtgta gggaatcctt ttggtgagca tcagcgcatc gtaccattct
     9181 tcgggcgtcc tgttgaagat gttcagcacc ttgcctgcct gggttaagag gtcctttgcc
     9241 ctgagcacca ggtcattcgc tttctggccg gcagggtggt tgtccagaaa cctgttgacc
     9301 tcccttatca cctcaaagat atgacccagt gcctgcgcag tattaaaatc gtcatccatt
     9361 gcttctctga acctgtccat gaagacggca agggtttctt ccagcgcatg gtctccggca
     9421 gaaggttttt gagtacccac tcccccttta aggaaatcct ccatccgggt agcggtggtg
     9481 tagtaccggt caatggagac ctctgcttct ctcaacagtt cgtcagagaa ttcgatcggg
     9541 ctcctgtaat gagtggagag gaggaaaaag cgcacaacct ctgcgtcaaa cttgtcaagg
     9601 atttccctta tggtgaagaa gttgcccaaa gatttggaca tcttctcccg gtcaatggtg
     9661 atgaagccgt tgtgtatcca gtatcttaca aacggctttc cggtgtatgc ctctgactgg
     9721 gccagttcgt tttcatggtg tggaaagata aggtccgcgc ctccaccgtg aatatcgaag
     9781 ctttctccta ggtgctttat ggacatggca gtacattcaa tatgccagcc gggccttccc
     9841 ggtccccagg ggctgtccca ggaaggttcg ccttccttcg atgacttcca gagggcaaaa
     9901 tccatgggat ttctctttcg ctcatccacc tcgaccctgg cgcctgcgag catatcctca
     9961 gtatcccttt ttgagagctt gccatagccg gggaatttat tcacctcaaa atatacgtcc
    10021 ccgtcaaccg cataggcgaa tccacggtcc atgagccctt tcacgatccc gatgatttcc
    10081 gccatatgct ctgtggcttt gggctccaca tcggcccttc cgacaccaag acggtccatg
    10141 tccctgtaat attccccagt atactttctt gccactgcat cccatgaaat accttcctgt
    10201 tttgccctgt tgatgatctt gtcgtcaatg tcagtgaagt ttctgatgta gttcacctga
    10261 aggcccctga aggcgaggta tctcctcatg acgtcaaaca cgatggcgct cctggcatgt
    10321 ccgatatggc agaggtcata cacagtgacc ccgcatgcat agatcccgac cctcccaggg
    10381 gtgaggggat tgaaatcttc cttctttccg gtgagtgtgt tataaacccg catcattcct
    10441 cccttcccct caagctgctc aattcttttt tgcagttcag ccacatgctt ctccatttcc
    10501 ctgaatttct cttccaccgg gtcaggcatg tgtacacggg ctaggctttc caccggccct
    10561 tcgtccacca tcctgagtac cttcttcttt atcacttttc ccggtacccc caccacgatg
    10621 gaatattcgg gtgcggagtc aaggaccacg gcattggcac ctatcttagc atgatctcct
    10681 atggtgattg cacccagtat cttggcgcct gtgcccacca ccacattatt cccgagtgtt
    10741 gggtggcgct tgcccttttc ttttccggtc cctcccaggg taacgccctg atacaatagg
    10801 cagtcttccc ccacctctga ggtctctccg atgaccacac ccattccgtg gtctatgaaa
    10861 aaaccgggtc ctatctttgc tgcagggtgt atctcaattc ctgtgagaaa ccttgataaa
    10921 tgggaaagca gccggggtat taccggtatc cccaggtccc agaggatatg gttaattctg
    10981 tggagtacta tcgcgtgaaa tccgggatag gcgaggacca cttcgaggcc attccttgca
    11041 gcggggtcgt ttctgaagac agacctgaaa tccctcttta tcgtgctcca aaaacccatt
    11101 ataaaggtta ttatacacta aatggatagc gtcataatct cgaaacaatc agaggtcaag
    11161 caacctcaga atccttatag tcgacaagat atttatatga tgtcaacatt tcaagaaagt
    11221 gaggttgtta caaaatctct caatagtttt cttttacaag gtgtcgtata agaatccttc
    11281 gcctgtccga cctctgcaga ctgtaaatat ctgtctattt gaatgttttg gccccttcct
    11341 ttatcatgta aggtatgtct gacgacatca agaccatgat cgccgattat atggagaagg
    11401 gatttctcga gaacatcatc gacatgttca aacacgacaa gggcctctat ccgttgattg
    11461 gtgagttaat gactgatgag cgcatgcggg tgagactcgg catttctgca cttgtggaaa
    11521 cactcgcaaa ggaagaccgc aacaccattg cacgggcgat tccgggaatc gctcacctcc
    11581 tcaggaatga aaacccgatc atcaggggtg acgctgcata tctcctcgga atcataggac
    11641 agcgggaggt tctaccgttt ctggtggagg cagcagatga taacaattcc ggcgtgagac
    11701 aaatcgtgag agaggcgata gaggagatag gagagacaga gggttaaacg tccaggcccg
    11761 agagcggata ttttcagaat tcttataatc gggatattac tatggcatat cgacatccct
    11821 taaatggcaa gacttgtcct cagatctctc aaaagggttt tttcataagg tgtcatacaa
    11881 gaattcttct cctgccaagc ccctgtcggg gaatgcggtg aactttgttg gaaaaaatat
    11941 gacgaataac acaattatca gcttatttct taacgctgcg ggaagatacc cggacaacac
    12001 cgccttcaac tactttgcgc aatcgtggcg gacaataacc tatggcgagt ttctcggaaa
    12061 gacaaggggc attgcatcgc atctgataca agagggagtg aggaagggcg acaggatcgc
    12121 cattattctc gaaaacaggc atgagtggtg cgcatcatat ctaggtgtcc tcatagcggg
    12181 agggattgcc gttcccatgg acgcgcagct gggatccaat gaggtcaaaa atcttctcaa
    12241 tgattcaggg gctgctcttg tagttcacag tgataggaca gagacgaatg taaagagtca
    12301 gatcaggggg atcaatgtcg attccacaga gtttcaggag atctgccgca ccccgtttag
    12361 ggggcactat ccaaacctca cggaagagga tctggcatcc atcgtctata cgtcagggac
    12421 aacaggcacc cctaaagggg tgatgctgac ccacaagaac ttctgctcag attcagaggc
    12481 cttgatcagc gcaggcattg tgacccatga ggacaatgtc ttgtcaatcc tccccctcca
    12541 ccacacctac gcgttcatgt gcaccttcct cgtccctctc tttctgggtg caaccattac
    12601 ttatcccctg ggaatgaaag gccctgaact catctcgact atgaaggaga ggggggtgac
    12661 ggtcctggtg gcagtgcccc agctcctgga gctcataaga aacggcatta tgaagaagtt
    12721 cagggagatg ccttctccgg tctctctccc gcttctcctt cttctaagaa tcagcggagg
    12781 actgcggaag agcacggacc tcaatatggg aagagttatc ttcgcctcag cgcaaagggc
    12841 attcggtgag aggttcaggt tctttgcgag tggcggcgca aggcttgatc ccatggtgaa
    12901 ggatgacctt gaggcattgg gctttactgc ccttgaagga tatggcctta ccgagacctc
    12961 tcctgtagtg accttcaatc cggtagagaa gaggaagtcg ggctctgcgg gaagacctct
    13021 cccaggggcg gagataaaga tactgaatcc ctcagggaca ggggagggcg agattgcgat
    13081 tcgaggcccc atggtgatga agggctatta caagcatccg gaagctactg cgcaggtgat
    13141 ccgtgacggc tggtttctga gcggggacct cggatatttg gacaacgaag gatacctctt
    13201 tatcaccggc agggcaaaag aggtgatcgt cctcagctca ggaaagaatg tgtatcctga
    13261 ggaagtggaa aaggcgtacc aggaaatacc ccttgtcaaa gagatctgtg ttacgggaat
    13321 tgaaaaagaa gggatagtgg aatcacttca cggtgtgatt gttccgaacc tagaccacgc
    13381 gaggaaggag cgcataggca atatctccga ttccctgaaa tgggcaatga acaaagttgc
    13441 tctcaaactg ccgccttata tgaggcttaa agggttcacg ctctcttccg agcctctgcc
    13501 cagaacgccc ctgggcaagc tgaggaggta tatggtaagg gacataatca gcaaggcgag
    13561 aggcgggagg cgggaggcga agggagagga tagtttatta ttgagagatg aaacaggaag
    13621 ggtggttgag gggtgcataa cgcctctttt gcgggaagcg atgccggtcc atttatcaga
    13681 caatcttgag cttgacctcg gcctcgattc tctccagagg atagaacttg ttgtggctct
    13741 ggaaaaggcg ttctccttca aactccccga gaccttcgca tcagaagtcc agacggtagg
    13801 ggagctcgtg gaacggataa aggagttcaa gacccggggg atttcaggtg ctgagaagcc
    13861 cctatgggaa gatatttttg ctgcagaaat tcccgaggaa gaaaggaaaa agatagggtt
    13921 atatcaggga agagtggaat gggcattcag cgctgtctgt atcaagctca tcaaattttt
    13981 tctgagggtg ttcttcaggc ttgaagccag gggcatcgaa aacattcccg cctttccgtt
    14041 catcatcgcc cccaatcatt gcagcaatat ggacggattt gtggtgggtc tatcgatccc
    14101 ccttgctgtc ttcaagagtc tctacttcca gggctaccag acatatttcc agggatggct
    14161 gccttccctc ttcgcgaggc ttgcgcacgt gatacccatt gatcctgaaa cctttctgag
    14221 caaggccctc cagctctctt cctatgtgtt aaagaaaaac cgtgcccttt gcatattccc
    14281 cgagggagga aggtcaattg acggcagtct cctggagttc aagaagggca tagggattct
    14341 tgccatggag cataatatcc ctgttgtgcc tgtattgata gaggggacct tcgaggccct
    14401 gccccgagga gcgctctggc caaagtttgt gaagataaca ctcacaatcg ggagacctct
    14461 ttacgcggca aaagtggatt tctccaaaaa acccgaggga atagacgagt atcagttctt
    14521 tgcgaatgag gtgagggaga gggtgaaggg actaaaggag tttccggcag atcaatagtg
    14581 gtcctgacct gaactgatca atagcaccac cactttcttg gtgagcagtt cactgactat
    14641 accacactgt ggacattgcg ccacagtaat tattaccgtt tctaatcttc gcaccatggc
    14701 cactattcaa tctgtggttg tccatgtcgt actcaacttt tcgcgaggat ggcctctggg
    14761 aggtttttcg acaaatgtgt agtgaaggat tagctctgtt gactcattta actcagctaa
    14821 tcttttcttg acttaccaat aaactcatgt ttatataata tatattatga tccttatttg
    14881 tttcactaat taggagttat ctcccctcag aagaaaccaa gggtatcatg ttacataaac
    14941 aagagtttat cgaaagtact attttagtat ttcctcgtgc ggttaagtcc tcatacagca
    15001 agggaaaccc gcatgcttaa acaatttact gactacgtaa ccttcacttt actagaactg
    15061 gaaccgacca gccatgcggg ggaagctctc aacttcttca tctacgatac gctcaagata
    15121 ttcacccttc tcactaccat tgtctttgta gtcgcaatca tccgggcgta cttcccgcct
    15181 gagaagacca agcggatact ggctcatgag agggaatata tcggcaacgt catggcggcc
    15241 ttacttggta tagtcacacc attctgctcc tgttcggcag tccctctttt tatcggtttt
    15301 gtggaagcag gaatacctct tggggtcact ttctcttttc tcatttcttc cccaatggtc
    15361 aatgaaatag cactgattat gctctggggt ctcttcggct ggaagatagc cctgatatat
    15421 accagcaccg gagtcctgtt ggcaattgcc tccggcatcg tcataggcag gcttaagatg
    15481 gaaaagcatg tccaggatta cgtctgggaa atgaaggtcg gaaactcgga gatcgtggag
    15541 acaagcttcg gtcagaagat cgattatgcc cgtaactata ctgccgagct tctgaaaaag
    15601 atttggccct atgtgatcat cggtattgga ttgggaggga ttgttcacgg ttatatcccg
    15661 actgactttg tggtcaaata tgcggggcct gacaacccct ttgctgttct gattgcagtt
    15721 ctgataggca tcccgcttta cagcaatgcc gccggcatga ttcctgtggt ccaggcactg
    15781 gtgggaaaag ggatggccat ggggactgtg ctggccttta tgatgtcggt aaccgcaatc
    15841 tcttttcccg aggcggtaat actgaagaat gtgctgaaga tgcgtcttat ttatattttc
    15901 ttcggtattg tggcggtggg aattattata gttggatatc tgttcaacac aatcttatag
    15961 gaggatatga aatggaaatc aaggtattag gtccgggttg tgcaaactgt cacaagatgg
    16021 aagagctggc aaagaccgcg gtgaaggaac tcggcgttga agcaagggtt gagaaggttt
    16081 ccgatattca ggaaatcatg aaatatacga tgtccacgcc gggccttgta gtaaacggca
    16141 aactcaaaca ttcaggaaag ccattaccta atattgaaaa agtaaagaca cttatcaaag
    16201 aggaggcatg accatgcagg atttgatgaa tatcttcaaa gccctttcag aagaaacaag
    16261 gctcaggatt ttaaagctgc ttgagcatgg agaactgtgt gtatgcgata ttgttgccgc
    16321 acttgaactt attcagccta aagtgtcttt ccatctgaat gtattaaaag aagcaggctt
    16381 tcttaaagac aggaaacaag gacgctggac ccattatcgc attgacgact cggatttctt
    16441 cagaaggttc ctgcttatgt cggtcctcga aagaattcat aataaagcgg tcagtgaaga
    16501 caacaaaagg cttgatgctt ttatgcagac taaaaagacc aaggcaacag ttgtggatat
    16561 tgagaagagg cgaggatgct gcggataata agcatgtcta aagaagatac taactttcac
    16621 ctataaccaa tctaaaggga gtcaactatg gataagaaaa aagtgttgtt tgtctgtgtt
    16681 cacaacagcg ctcgaagtca gatggcagag gcgtttctca atgctatggg cggcgataaa
    16741 gttgttgctg aaagcgcagg attggagcca ggggtgttga atccgatagt cgttgacgtg
    16801 atgaaggaag tggggtttga tatctcaaat aacaagacgc agggtgcttt tgactgcttc
    16861 aaggcaggga aattgtatca ctacgtgatt acagtctgtg atgaaacaaa tgccgaaaga
    16921 tgccctatat ttcccgggct ggcgaaaagg ctccactggg gttttgatga tccctcaaag
    16981 cttcaaggaa gccgtgagga aaagcttcag gggacaagaa ttatcaggga ccaaataaag
    17041 gccaagagag aagagtggat ctcacaacaa caataaaaga gatgaagaga aaggatagat
    17101 tatgaaccca actataacta gaaggctttc ttttcttgat cgatatttga ccctgtggat
    17161 attctccgca atggctgctg gcgtcggcct tggttacttt attcctcaaa cggaaagtct
    17221 gattaacagc tttcaggtcg gtacaacaaa tattcctatt gccatcggcc ttatcctcat
    17281 gatgtatccc cctcttgcaa aagtgaaata tgaggaactg ggagaggtct ttagaaacaa
    17341 aaaagtgctt acgctgtccc ttgtccagaa ctgggtaatc ggcccaatcc tgatgttctt
    17401 gcttgcgatt atttttctgc gcagttatcc ggaatacatg gtggggctca tcatgatagg
    17461 ccttgcacgc tgcatcgcca tggtcatagt atggaatgat cttgcttgcg gtgaccctga
    17521 atatgctgcg ggacttgtcg cattcaactc catctttcag gtcctcttct tttcagttta
    17581 cgcctatgtt tttattaccg tccttccgtc atgggtcgga ctaaagggtg ttgttgtaaa
    17641 tgtcactatc ggccaaatcg caaaaagtgt ttttatctac ctcggcattc cctttcttgc
    17701 ggggatgata acaaggttca gcctactcaa aatcaagaat cgtgagtggt acgaaaaagt
    17761 atttatcccc aggataagcc cgatcacact catctttctg cttttcacaa tcctcgtcat
    17821 gttctcgctg aaagggaatt acattgtcag gctccccctg gacgtgatac gaattgcgat
    17881 tccgcttctt atctattttg tttttatgtt cctcgtctcc ttctatctcg ggaaaaagat
    17941 cggggcggac tatccgaaga cagccacgct ctcctttact gcggcgagta ataactttga
    18001 gcttgctatt gctgttgctg ttgctgtttt tggactcaat tcaggtgctg cctttgccgc
    18061 ggtcatcggc ccgctcgtgg aagtgccggt tttaatcagc ctggtaaatg ttgccctcta
    18121 tttccagaag aggtatttca ccattgagta ctgcagggta aaaggcgttg aatcacctta
    18181 aaatgttcaa ggaaaaacca taagatatgg aggagtaatg aaaaaaggat tgatgcttct
    18241 cgttgcccta ataacgctct tgacgctatt cgaatacagc aatgcaagcg acgttgatag
    18301 catgctgagc aatgcacgaa aagatgggaa ggcggtcatg ttagagattg ggtctgaggg
    18361 atgtgtcccc tgcgagcaga tgaagcctgt aatggaaaaa ctaagaacta attacaaagg
    18421 caagctggaa gtcttctttg tcgatgtacg taaggacaag gagacctcga gaaagttcgg
    18481 tgtccatgtc ataccgacac aggtttttct tgataaaaac ggtaaagagt ttcatcggca
    18541 cttcggcttt tacgagtatg aggagattgt ccccgttctg aagaaagccg gcctttaacc
    18601 gatgtgcaat gcctaaacag agggaaaaag atgaaaattg caattcttgg tgatatccac
    18661 ggcaaccttg aagcattgga ggttgcttat accacagcag tgtccgagca tgttgagaaa
    18721 atataccacc ttggagatat cggtggttat gctccgttcg tgaacgaggt ggtcgatttc
    18781 ctaatcgagc acggcattgc aggcatccaa ggaaattacg acgagacggt tgccaatgac
    18841 cgtgagcatt gcggatgcaa atatgaggac ccgatccagg aggaaatggc gaaccggtcc
    18901 ttcgaatgga ctaagaggca tgcatcgcag aagaccaaag agtatctgaa gaaacttcct
    18961 ttcagcattt cgtttcaggc tgaggggaag aaggtactgc tctttcatgc gacacccggg
    19021 aaaaacaacc tctattggta cgaagacagg cctgagaagt ttttccgtga gatggcaagg
    19081 aaagtggacg ccgacgtaat gatttacggc cacacacata aaccgtatcg gatggacatg
    19141 gataacaagg ttttcataaa tgcaggaagc gtcgggaagc ccaaagacgg agatccgagg
    19201 gcatgcgtag cccttattga aataaccaaa ggcgccgtgg taacaaattt ccttagagtt
    19261 ccctatgata ttgcaaagac gaccgaggct attgtagcca gtggactgcc tgcctacttt
    19321 gcagataaat tgaaagaggg cagataggat gttggaatcc ctgttgggca atatacagac
    19381 agtaatccag aatcggcatg gcctcgcgtt tgttgctgta tttatcggtg gcatcatatc
    19441 tgcagcaagc ccctgtgttc ttgcagccat tcctttgata atcggctatg tcggcggtta
    19501 ttcggaagga aacaaaagaa gggctgctct ttactctctt gtctttatcc ttggcttgtc
    19561 gcttaccttt acccttcttg gtgcagcagc atcaatcatg ggccagtttc tgggattcat
    19621 gggaaaatgg ctctaccttg gtctggcact gatagctgtc ttgatgggtc ttcagcttat
    19681 gggtatcatc gaaattcctt tacccttcca gaagacaaga gcagttaaaa caaagggact
    19741 cctcggggcc tttcttctcg gcatgttgac cggaacggtc tcatctccct gtgcaacgcc
    19801 ggtccttgcg gtcatcttgg cctatgtctc gactcaaggc gatatgatat acggcggatc
    19861 actcctcttt gtctatgccc tcggacactg cgcgcttata tttatggccg gactgtctgt
    19921 cggggtcacg gaaagtattg ttcgctcaag aggcatcaga aacttctccc tttattcaaa
    19981 gaggttcagc ggcgcgattc tggtcgctgt tggaatatac ttcggaataa caaatatctg
    20041 agtaatgaat gaaaggtggg atggctaggg gagctggata tgccataatg ataaccgtga
    20101 aggggaaaag acctgccggg tatattaatt ggcatatgaa gagataatag agaagataat
    20161 gacaatattg gaggcgcatg atgctattat ccggttatat gaaggacgta tctctcccag
    20221 tgtgcaattc gagttttcag tccctgcact gtattgcata tctgaatgaa gatatcacag
    20281 cagtccttcc atatcttaat gcagagcttg gaggccatca atatataaaa gagcctcctt
    20341 ctgtgacttt caaaattaac ggcagattaa taacaataca tcccacaaga attgcggtca
    20401 atgctttgga gagcgtggag caagccgata agattttgga atggctcagg aaacagatta
    20461 acgatatatg ggaaagacgc gaggaaattg aaccatcata taaacgtgtt gcatctccta
    20521 cgcttctcga tatcctgaaa cttcttccaa ggacaaattg taaaaaatgc ggccaatcta
    20581 cgtgccttgt atttgcaact caaattatgg agggcggcaa aggacctgaa cattgtcctg
    20641 cacttaacgc ggttgaacgt cggaaactcg aacactatct caatcaattc agcattactg
    20701 gaggagtgat gtcaaagtga atacaagaaa taaaagaaca cataacaaag gcatcgagag
    20761 ggatgcgaga atcaatgaaa ggcggaagca acatgaatag cagcgtttgc tgaatattgg
    20821 agctgcaggt ccaatcaggg tgggagaagg acttcagggg tcttatggca gagatggtca
    20881 cagcaacaca gtcgaatgaa actgggacgg ttaattacga gtggagcaca agtgcggatg
    20941 gcaaactatg tcacatttat gagcgttatg tcgactccgc ggcggtgatg actcatctag
    21001 gcacgttcga cgagcgcttt gccgggcgct ttctagaggt cttgaaacct gttcgctttt
    21061 ttgtatacgg ctcacctagc caggaagtta aggatgcgtt agcgggattc aaccccatcc
    21121 atatgaagtc agtgggcggc tttagccggt agcgcgacgg acc
//
LOCUS       DATKOU010000143         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5578642, whole genome shotgun sequence.
ACCESSION   DATKOU010000143 DATKOU010000000
VERSION     DATKOU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5578642"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VMU21_06540"
     CDS             <1..474
                     /locus_tag="VMU21_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77218.1"
                     /translation="KTAAKPAKKVATTGKKPVAPEFTAKPAIEITATFPDTMGLTAKV
                     AAALAAENVNILAGTGYSASGMRRKAIFTLIVDNYAKAERALEKIGADDLHESSVIMV
                     ETPNKVGALERVSKIIANSGINIYYFYATTSSGKTATSVFKTADDRKTIRVLCEA"
     gene            complement(549..1775)
                     /locus_tag="VMU21_06545"
     CDS             complement(549..1775)
                     /locus_tag="VMU21_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77219.1"
                     /translation="MIDFTQRRFAIRFSLILVIFTVFILAVIAVYFHQTIVHAVKRHM
                     KEVIQRDFVDQFKRAGIDTSKPAWEEYYFQILNGKGDIVVASRSSATFYPVLNRDLLG
                     SAFTGRGGFETLEIREESYLISYFPLNERFAGRIAVPFASGTQYERNFYRVILIIFPG
                     ILLLSYLVSRYLVRQAMKPVSDAFTYQESFSSSVSHELRSPFASLKGNLEVTLRKERS
                     PEEYRKAIAFGLTEVDRIISLLNDLNLLASSRFKPLDLFREQVDLAVLIAELYESILP
                     RAKAKNINIENKVDSVSPCVCDKGLIRRVLENIFTNAVNYTPEGGLIVIRVFEGMGKL
                     LVNVSNTCTGIDRNELHDLFEPFYRGKSTTKGKTQGKGLGLFIARYIVRSHGGEVTAN
                     LTDTGMFSLTVSLPLK"
     gene            complement(1768..2463)
                     /locus_tag="VMU21_06550"
     CDS             complement(1768..2463)
                     /locus_tag="VMU21_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO77220.1"
                     /translation="MYMRILLVEDEEGVANFIKKGLVEEHYAVDLAVDGEEGRVLALA
                     NRYDLIILDVMIPGINGIELCKEIRQKKIQTPVLMLTAKDSLKDKVAGLDSGADDYLT
                     KPFSFEEFIARIRALLRRRQDALVELRHKDLRIDVLSHRVFAGENEVVLRPKEYAILV
                     YLVRNRGRVLSRTQIIENVWGYDFNPNTNIVDVHIKSLREKIGEFVSPDFIRSVRGTG
                     YMLENAPESDSRD"
     gene            3113..>3358
                     /locus_tag="VMU21_06555"
     CDS             3113..>3358
                     /locus_tag="VMU21_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HUO77221.1"
                     /translation="MPKELLDKKDIDRILSRMAHEILERNKGAKGLCLVGIQRGGVHL
                     AKRLAHKIREIEDKDVPVGSLDIAFYRDDLSIRKEQPQ"
BASE COUNT          861 a          801 c          883 g          813 t
ORIGIN      
        1 aaaaccgccg caaaacctgc gaagaaagtg gccacaacgg gaaagaaacc cgtggcgcct
       61 gagtttaccg ctaagccagc catagagata accgcaacat ttccggacac catgggattg
      121 accgcaaaag tggccgcggc cctcgctgcg gagaatgtaa acatacttgc cgggacaggg
      181 tactcggcga gcggcatgcg cagaaaggcg atcttcaccc tgatcgtgga caattatgcc
      241 aaagcagaga gggccctcga aaagatcggc gctgatgacc tgcatgagtc gtcggttatt
      301 atggtagaga caccgaacaa agtgggggcg ttggagaggg tatcaaagat cattgcgaat
      361 tccgggatca acatctatta tttttacgcc actaccagct cagggaagac agcgaccagc
      421 gtgttcaaga cagccgacga caggaagacg attagagtcc tgtgcgaagc ctaaacggga
      481 tattcccgca caagcccact tcactcacta tcggagcggt cttgagagcg cggatccttg
      541 gccgaccctc atttcagcgg aagggagacg gtgagcgaaa acattccggt atctgtgaga
      601 tttgcggtca cctctccgcc gtgggaacgg acgatataac gcgcaatgaa gaggccaagg
      661 ccttttccct gagtcttgcc cttggtggta cttttcccgc ggtaaaacgg ctcgaagaga
      721 tcgtgcagtt cattcctgtc gatcccggtg caggtatttg atacattgac gaggagtttc
      781 cccatgcctt caaacacgcg gattacaatc agcccgccct cgggagtgta gtttacggca
      841 ttagtaaata tattctcaag taccctccgt atcaggcctt tatcacacac gcagggactg
      901 acagagtcaa ctttgttctc gatattgatg ttctttgcct tggcgcgggg cagaatgctt
      961 tcgtagagtt cagcgataag caccgcgaga tcaacctgct ccctgaaaag gtcgagaggc
     1021 ttgaaccggg aagaggcgag gaggttgaga tcattgagca ggctgattat acggtctacc
     1081 tctgtgagac caaaggcgat tgcttttcgg tattcttcag gagaccgctc tttccttaac
     1141 gtcacctcca ggtttccctt aagggaagcg aagggggagc ggagctcatg gcttacactc
     1201 gaggagaagc tctcctgata ggtgaaggcg tctgataccg gcttcatcgc ctgcctgacc
     1261 agataacggc tcacgagata ggagaggagg agtattccgg gaaagatgat gaggataaca
     1321 cggtaaaagt ttctctcata ttgagtcccg gaagcaaagg gcacggcgat ccttccggcg
     1381 aaacgctcat taagggggaa ataggagata agataggatt cttcccggat ctccagggtt
     1441 tcaaaaccgc cgcgtcctgt gaaggcgctc cccaacaaat ctctgttgag gaccgggtag
     1501 aacgtggcag agctcctgga tgcgaccaca atatcacctt tgccgttcag aatctgaaaa
     1561 tagtattctt cccacgcggg cttggaggta tcaatgccgg cacgtttgaa ttggtcgaca
     1621 aagtctctct gaataacctc tttcatatgc cgctttaccg catgcacgat ggtctggtgg
     1681 aaatacactg cgatgaccgc aaggatgaag actgtgaaga tgacgaggat caatgaaaag
     1741 cggattgcaa aacgtctctg tgtgaagtca atcacgggag tctgattctg gagcgttttc
     1801 cagcatgtat cctgtccctc ttacactcct gataaaatcg ggtgatacga attcaccgat
     1861 tttttcacgg agtgatttga tatgcacatc cacgatgttg gtgttggggt taaaatcata
     1921 accccataca ttttcgataa tctgggttcg tgaaagcacc cgcccccgat ttctcacgag
     1981 gtagaccagg atggcatact ccttcggacg gagaacgact tcgttctcgc ccgcaaaaac
     2041 cctgtgggac aggacatcga tcctgaggtc tttatgccgc agttccacaa gggcatcctg
     2101 tcttctcctg aggagggccc ttatccgtgc gataaattct tcaaaggaga aaggttttgt
     2161 gagataatca tcagcgccgc tgtcgaggcc ggccaccttg tcttttagcg agtcctttgc
     2221 agtgagcatc aagaccgggg tctgaatctt tttttggcgg atctctttgc agagttcgat
     2281 gccgttaatc ccggggatca tgacgtcgag gataataagg tcataccggt tcgccaaagc
     2341 aagcactctg ccctcttctc cgtccacggc gaggtcgacg gcataatgtt cctctacgag
     2401 tccctttttg atgaagttgg ctacgccttc ttcatcctcg acgaggagga ttcgcatata
     2461 catagtctaa tccaattagc cttattttga gaagagtgtc ttccgggcct tgatgccctt
     2521 tggtcattag agagggtgga gggaaacttc cctccaccct ctcgtgtgca tgagaacaga
     2581 agtgcctgac agacagaccc ttctgttcat tcccatttgc tgcttacaga ttacctgcgt
     2641 cgtgcctgtc acggtgtcgg aacgcttgct gagaatttta ttaacgctca taaaccctga
     2701 aatcccttcc gaaaccgtga acatgaagga ggtgcaattt ttagggatta tttttttgtc
     2761 ctcctgtctt tttatagagt atgaaccaag aatattaaaa tcccattaac tgccaatgaa
     2821 tgtttcttaa taatgtgtta ttccaaacca gacctacacg aagtgcgtta atggagcctt
     2881 ttaacagagg gacgttcagg aaataagcag gattggtttc cgggattgat taatggttga
     2941 gaccgggaaa gcccaggccg cattgacagc gttctttctg ttatggttta atagtgctgc
     3001 ctttaagggc agcccggtga ggctgtgaag gcaaaggagc ccaagctgct tacattatta
     3061 caagataagc cttcctcatg atacgtgggg aaggcttttt ttatgcctga acatgcccaa
     3121 ggaattatta gataagaaag acatcgaccg gatactctcc cgcatggccc atgagatcct
     3181 ggaaaggaac aagggcgcaa aaggcctttg cctggtggga atacagaggg gaggagtgca
     3241 ccttgcaaag agattggcac acaaaattag agagatagag gataaggatg tgccggtggg
     3301 ctcgctggac atcgcttttt atagggatga cctgagtatc agaaaggaac agccccag
//
LOCUS       DATKOU010000144         9012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3652791, whole genome shotgun sequence.
ACCESSION   DATKOU010000144 DATKOU010000000
VERSION     DATKOU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3652791"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VMU21_06560"
     CDS             <1..430
                     /locus_tag="VMU21_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HUO77222.1"
                     /translation="LSAYKWNVVEVYPNVFFETYVLEDWGNAFNEGEVRDAWAGYCFG
                     MRDHFSILHNGDVILCCIDFEGKTTVGNLRHSSLREILSSDELGKIMDGFRKFQLVHP
                     HCKQCLGSKSFSSWLLKPLLSIAALKVLKPFFYKRVRIYE"
     gene            544..1278
                     /locus_tag="VMU21_06565"
     CDS             544..1278
                     /locus_tag="VMU21_06565"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HUO77223.1"
                     /translation="MSFYSRPLYQDVATNWKKISILYLLLLLAVCLIPLTFKTRASVS
                     DYVLREAPKIIKQFPVVSISKGQVSVDVPMPYTIKDPENNAPLVIIDTTGQVTSLAGS
                     NALVLLTKTKLFFKRGPKESRILDLSDIDSLVINQSRLYDWMETFLEYFAFVLYPVAL
                     FFTFLFRISQALVFALIAISFSSYLKLNFKYSALLSIAIVSMTPAIILDTLYNYLDMA
                     IPFWWLIDFALALAYLFFALKLNAEK"
     gene            complement(1323..2162)
                     /locus_tag="VMU21_06570"
     CDS             complement(1323..2162)
                     /locus_tag="VMU21_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUO77224.1"
                     /translation="MKKFPFLHLVLFVLTFFSTLIAGALFQKGVNIFNEPWRIIEGLP
                     FALTLMTILLSHELSHYFASKKHHTKATLPYFIPAPSLIGTFGAFIKMKSPIITRKAL
                     IDIGAAGPIAGFVLSVIAVIAGLRYSSVIPVADTESILSLGDSLLFTLLSRLVLGVPP
                     ASYEIVLHPVAFAGWIGLFVTSLNLIPIGQLDGGHIAYAFLGERHKFISLTLVGTLLL
                     MGFFFWEGWAFWGVVMIVLGLKHPPVIYWEAPLDRTRKIVGMLALIIFILTFIPSPFR
                     ILE"
     gene            complement(2159..3556)
                     /gene="rimO"
                     /locus_tag="VMU21_06575"
     CDS             complement(2159..3556)
                     /gene="rimO"
                     /locus_tag="VMU21_06575"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HUO77225.1"
                     /translation="MPKVSIVSLGCPKNLVDSETLLGNLGKEGFENTPNAEDAELVLV
                     NTCGFIEEAKRESIEEILKLRHLRGEGKKLLVFGCLAKRYGNELMKEIPEIDALWGVG
                     EEEKIVDYCKKAVGSKQPLVDDQGNNDKRGPMPGKANLERGSYAYLKIAEGCSRGCTY
                     CVIPDIRGPYRSTEPEKILRKAEEHIASGIRELVLIAQDIGSYGREFGGYTLVSLLKD
                     MASITGDFWIRLLYLYPTAINEELLSLISHEHKICKYLDVPLQHSEDRILRAMGRTGT
                     KESYSDMIRRIRDEVPGAALRTTFIVGFPGETGDEYQGLKTFIEETRFERLGVFVYSK
                     EEGTPAAGMKGNVPRKVKEERRDEIMRVQSRISLDINKTLVGQRFRVLVDETDGDTII
                     GRLSSQAPEIDGVVIIEKKDKAMSTVHGASGRKKDKNASRLTLDPSRLLRAGEFVEVK
                     IKEAYDYDLKGELVE"
     gene            complement(3549..3977)
                     /locus_tag="VMU21_06580"
     CDS             complement(3549..3977)
                     /locus_tag="VMU21_06580"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HUO77226.1"
                     /translation="MALRDKIRLLFEVKDTPHRISMAFALGIFIGISPLFGIHTILGI
                     SLAHLFRLNRFVTIIAVYVTNPWTIIPIYTFCTWVGAKCLGIKQILPDIDWGHITFSM
                     LLKDLGPLLMPFLLGTVLIGLLASVASYVIIYRLLKRRNA"
     gene            complement(3968..4948)
                     /gene="thiL"
                     /locus_tag="VMU21_06585"
     CDS             complement(3968..4948)
                     /gene="thiL"
                     /locus_tag="VMU21_06585"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HUO77227.1"
                     /translation="MRLNERGELSLLKEIQKRFKGRAKDIATGIGDDCAVVIPRKEKL
                     LLTTDMMVEGVHFDFAFTTHFQIGFKIISVNVSDIFAMGGTPRFVLLDIAAPEDTDED
                     SLDSLFEGMEKAFRHYHITLVGGDLSASESFVVSATLIGYAKKPVLRSGARPGDGIYI
                     TGNLGDSACGLELLKRIKRPVSLETGEKIHTPLGWSTMEPLLRRHLLTEVKKPGKLAG
                     IATAMIDVSDGLFIDLYRLCEESRVGARICMKQLPLSIQMRKAASFLGREPDVFATSG
                     GEDYELLFTAPPRRRVNAACIGEITDSERVIVERDGSERPFSLEGYQHWH"
     gene            complement(5112..7484)
                     /gene="lon"
                     /locus_tag="VMU21_06590"
     CDS             complement(5112..7484)
                     /gene="lon"
                     /locus_tag="VMU21_06590"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUO77228.1"
                     /translation="MAEIEQKDEKEVEIPDTLPVLPVRDIVVFPYMILPLFVGREMSI
                     KAIDQALNTNRMVMLVTQRDLNMENPTQEDLYAIGTVGIIMRMLKLPDGRVKILVQGV
                     SKARVLGFTQTEPFYTATIEKITDQKVEEMTIETEAIMRTVKEQLDKAVSLGKTILPD
                     IMVVVENLEEPGRLADLVASNLGLKTEQAQEILEIIEPVQRLKKVSDILSREIELLIV
                     QQKIQTEARGEIDKTQREYFLREQLKAIQKELGEIDERGEEINELRKKIEEAKMPDKV
                     LKEAEKQLKRLGKMHPDSAEAATVRTYLEWMVEIPWSRSTKDKLDIKAAEKVLNKDHY
                     DLEKVKERILEYLSVRKLKEKMKGPILCFIGPPGVGKTSLGKSIARSLGREFVRMSLG
                     GVRDEAEIRGHRRTYVGALPGRIIQGMKQAGTNNPVFMLDEVDKIGMDFRGDPSSALL
                     EVLDPEQNNAFVDHYLGVPFDLSNVMFITTGNLVDTIPGPLRDRMEVIYLSGYTAEEK
                     LGIAKNFLVPKQLEEHGITKKILKMTDGALLLVISQYTREAGVRNLEREIANLCRKVA
                     KRIAEGGQKNFVITPRNLHRFLGVPKYLPEEEMEKDEVGVSTGLAWTEAGGDIIYVEA
                     TTMKGKGGLTLTGQLGDVMKESAQAALSYIRSRAKGLGIKDDLFSRTDLHIHVPAGAI
                     PKDGPSAGITMATAIASALTGKPVNKSVAMTGEVTLRGRVLPIGGLKEKTLAAKRMGI
                     NRVILPSRNKKDIEDIPKYIKKDMQFILVDTMDDVLKVALKTDGRAKGKA"
     gene            complement(7533..7898)
                     /locus_tag="VMU21_06595"
     CDS             complement(7533..7898)
                     /locus_tag="VMU21_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUO77229.1"
                     /translation="MSLRSGLYFHKETAGYLPLLDLYETDDSLILEMDLPGIDPEDIL
                     IKVYEDLLIIEGIKRKTDEEKGFRYLCMERRFESFRRMVKIPVPVNSVAGKASYSDGV
                     IIVTFPKIKDRVIKIKIEK"
     gene            complement(7900..8835)
                     /gene="galU"
                     /locus_tag="VMU21_06600"
     CDS             complement(7900..8835)
                     /gene="galU"
                     /locus_tag="VMU21_06600"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HUO77230.1"
                     /translation="MTEKRIKKAILPAAGLGTRFLPATKASPKEMLPIVDKPMIQYAV
                     EEAISCGIEDFIIITGKHKRAIEDHFDSAYELEEKLKNAGKKKLLDEINKLSHINFAY
                     IRQRVALGLGHAILCAKPFVKDEAFAVILSDDVIDPDYPLLKEMIRVYEEVDCPVIAL
                     EAVPKAEVSKYGIIDGVAEGDVYRIKDLVEKPKAEDAPSNLAIIGRYILTPDVFKILE
                     KQRAGAGGEIQLTDALRQLLRKRSIYGFPITGTRYDAGDKVGFLKATVDFALKNLEVS
                     EPFRAYILEKAMSMERSTTGGKGVKGAGVLRGAGG"
BASE COUNT         2134 a         2480 c         1918 g         2480 t
ORIGIN      
        1 gctttccgca tataagtgga atgtggtgga ggtctatccc aatgtgtttt tcgagaccta
       61 tgtgctggaa gactggggga atgcctttaa tgagggtgaa gtgcgagatg cttgggcagg
      121 atattgcttc ggcatgcggg accacttcag cattctccat aacggggacg tgattctctg
      181 ctgtatagat ttcgaaggaa agacaacggt gggcaacctc cggcattcat cccttcgtga
      241 gatactctcc tctgatgaac tcgggaagat tatggacggc ttcagaaaat tccagctcgt
      301 tcatccccac tgcaagcagt gtttaggaag caagtcattc agttcctggc ttcttaaacc
      361 cctgctctct attgcagcac tcaaggtgct taagccattt ttttacaaac gcgtgcgcat
      421 ttatgaataa agaaagtgcg gcagactaat ctttgaggtt atgaaaaaca aaaaggcgct
      481 cttaggcaaa tctcagggaa ccggaacacc atgaaaagat acagcacggc gcacccctta
      541 tttatgtcct tctactcaag gcccttgtat caggacgtgg ccacgaactg gaagaagata
      601 tccatactct atctccttct gctacttgcg gtctgcctga tacccctcac atttaagacc
      661 cgtgcgtcgg tctccgatta cgttctgcgt gaggccccga agattataaa acagttcccg
      721 gtggtgagca tctccaaggg gcaggtatcg gtggatgttc ccatgcccta taccatcaag
      781 gatcccgaga ataacgcacc tcttgtaatc attgacacca cagggcaggt gacctcttta
      841 gccggttcaa acgcattggt cctcctgaca aaaacaaaat tgtttttcaa aagaggtccg
      901 aaggagtcga ggatattgga cctctctgat attgacagtc tcgtcatcaa ccagtccagg
      961 ctctacgact ggatggagac attccttgag tactttgcct ttgtccttta tcctgtcgcc
     1021 ctttttttta cttttttatt caggatatct caggcgttag tttttgcatt aatagcaatt
     1081 agttttagta gctacctaaa gcttaacttt aagtatagtg cgctgttgag tatcgcaatt
     1141 gtatccatga cacccgccat tatcctcgac accctttaca actaccttga catggcgatc
     1201 cccttctggt ggctgataga tttcgccctt gccctcgcgt atctcttttt cgctctcaag
     1261 ttaaacgcag agaaatagcc cgaggcatct ctcaaaatca tgaatgctgc tcaaagatat
     1321 gctcactcaa gaatcctaaa gggagaggga atgaatgtga ggataaatat tatcaaggct
     1381 aacatcccca caattttcct agtcctgtcc aagggagcct cccagtagat caccggggga
     1441 tgctttaaac ccagtacaat cataaccaca ccccagaatg cccacccctc ccagaaaaag
     1501 aaacccataa ggaggagagt tcctacaagg gttaaggata tgaatttatg cctctcccca
     1561 agaaaggcat aggcgatatg ccctccgtca agctgcccta tggggatgag gttgagcgac
     1621 gtgacgaaaa gtccaatcca tcctgcaaat gccacaggat gcaacacaat ttcgtaggat
     1681 gcaggaggga ctcctagcac aagacgtgaa aggagcgtga agaggagaga atcccccagg
     1741 ctcaatatac tttcggtatc agccacaggg attacgctgg aatacctcag cccagcaata
     1801 actgcgatga ccgaaagaac aaaacctgca atgggacctg ctgcacctat gtctataagg
     1861 gccttccgcg ttataattgg tgacttcatc ttaatgaagg caccaaatgt tcctatgagt
     1921 gaaggcgcag gaataaaata gggaagagtg gccttcgtgt ggtgtttttt tgacgcaaaa
     1981 taatgggaga gctcatggct caggagaatc gtcatgagag ttagcgcgaa gggcaatccc
     2041 tcgataatcc tccatggttc attgaagata ttcaccccct tttgaaaaag ggcacctgca
     2101 atcagggtcg agaaaaacgt gaggacaaaa agaacaaggt ggagaaaggg gaatttcttc
     2161 actcaaccag ttctccctta aggtcataat cataggcttc cttgattttc acttccacaa
     2221 actcgcccgc ccttagcaaa cgtgaagggt caagagtgag ccgcgacgca ttcttatctt
     2281 ttttcctccc acttgcacca tgcactgtgc tcattgcctt gtcctttttt tcaatgatca
     2341 ccactccatc tatctccggt gcctgggatg agagccgtcc aattatagta tcgccgtccg
     2401 tctcatccac aagtaccctg aatctctgtc ctaccaacgt cttgtttata tccaaagaga
     2461 tgcgggattg caccctcata atctcatcgc gtctctcctc ttttactttt ctcggcacat
     2521 ttcccttcat tcccgccgca ggtgtcccct cctcttttga gtagacaaat acccctagcc
     2581 tctcaaatct cgtctcttca ataaaggtct tcaacccctg gtactcatca ccggtctctc
     2641 cgggaaatcc cacaataaat gttgtcctga gtgcagcccc cgggacttcg tctcttatcc
     2701 tcctgatcat gtctgagtag gactcttttg ttcccgtcct acccatggcc ctgaggattc
     2761 gatcctcgga gtgctgcaat gggacgtcca ggtatttaca tattttgtgc tcatgggata
     2821 ttaacgacaa cagctcctca ttaattgcgg tgggatagag atagagaaga cgtatccaga
     2881 aatcaccagt gatggatgcc atgtccttaa gcagggaaac aagcgtatat ccgccaaatt
     2941 ccctgccata gcttccgata tcctgggcaa tgagtacaag ctccctgatt ccactcgcga
     3001 tgtgctcctc agctttcctc aatatctttt cgggctctgt gcttctgtac ggtcccctga
     3061 tgtcagggat gacgcaatac gtgcaacccc tgctacagcc ttctgcaatc ttaagatatg
     3121 cataggaacc cctctccagg ttagcttttc caggcatcgg tccgcgttta tcattgtttc
     3181 cttggtcatc cactaacggc tgtttgcttc ctacagcctt tttgcagtaa tctactattt
     3241 tctcttcttc ccccacaccc cagagggcgt caatctctgg aatttctttc atgagttcat
     3301 tcccatatct ctttgccaga catccgaaca caagcaattt tttcccctct cctcttaggt
     3361 gtcgaagctt gagtatctct tctatcgact ctctttttgc ctcttcaata aaaccacagg
     3421 tattcacaag aacaagttca gcatcttccg cattcggggt attctcgaag ccttctttcc
     3481 cgagatttcc caaaagggtt tcgctgtcca cgagattctt tggacatcca agactcacaa
     3541 tggatacctt aggcatttct cctcttgagc agtctataga taatcacgta gcttgccacg
     3601 gaggcaagaa ggcctataag caccgtcccc aaaagaaacg gcataagtaa agggcccaga
     3661 tcctttaaca acatgctgaa ggtgatatgt ccccagtcaa tatcaggaag tatctgcttt
     3721 atgccaagac actttgcccc cacccaggtg cagaacgtgt aaatggggat tatggtccat
     3781 ggatttgtga catagaccgc gatgatggtg acgaatctgt tgagcctgaa gagatgcgca
     3841 agtgagatcc ctagaatcgt atgaatacca aagagcggtg aaataccgat aaagataccc
     3901 aatgcaaagg ccatggatat cctgtgaggg gtgtccttca cctcaaataa tagccttatc
     3961 ttgtccctta atgccaatgc tggtacccct ccagagaaaa tggcctttct gaaccatccc
     4021 gttcaacaat aaccctttct gaatcagtaa tctcaccgat acaagcagca ttcacccttc
     4081 gacggggcgg ggcggtgaag agcaactcat aatcctcgcc tcctgatgtg gcaaacacat
     4141 cgggttccct acccaaaaaa gaagcagctt tcctcatctg aatagagagg gggagctgtt
     4201 tcatgcagat tctcgccccc accctgcttt cttcacaaag ccggtagagg tctatgaaga
     4261 gtccgtcact cacgtctatc atcgcggtag cgatgcccgc aagcttgcca ggctttttca
     4321 cctctgtgag aaggtgtctc ctgagaaggg gttccatagt ggaccatccc aggggtgtgt
     4381 ggatcttctc ccctgtttca agggacacgg gcctttttat cctcttcaat aattcaaggc
     4441 cgcatgcaga atcgcctaga ttcccggtaa tataaatccc gtcaccagga cgtgcaccag
     4501 accgtaagac aggctttttg gcatacccga tgagggtcgc ggaaaccacg aacgattctg
     4561 atgctgacag atcacctccc acaagcgtga tatgataatg acgaaacgcc ttctccatgc
     4621 cttcaaaaag ggaatccagg gaatcttcat cggtatcttc aggcgccgcg atatccaaaa
     4681 gaacaaacct gggagtcccc cccatcgcaa agatatcgct cacattcacc gatatgatct
     4741 tgaacccgat ctgaaagtgt gtggtgaacg caaagtcgaa gtgaactcct tcaaccatca
     4801 tatccgtggt caggaggagc ttttcttttc ggggaattac caccgcacaa tcgtcaccta
     4861 ttcctgtagc aatgtccttt gccctgccct taaacctctt ttgtatctcc ttaagcaggg
     4921 aaagttctcc tcgttcattc agtctcatat tcctcgcgac aagttactaa aatgaatccc
     4981 ccttggtcct cccctggtaa gggaggctta gggggtctta acgttcagtc ctaatgcagt
     5041 aagaatgcag aggttacgca ctttcccgga aacaggccga agagaattca gcattccctc
     5101 gcgcccagag gctatgcctt tccttttgct ctcccgtcgg tcttcagggc cactttaagg
     5161 acatcatcca tggtatccac caggataaac tgcatatcct tttttatgta tttcgggatg
     5221 tcctcaatgt ccttcttgtt tcttgaggga aggattacgc gattgattcc catcctcttt
     5281 gcagccagag ttttttcctt gagtcctcct atgggaagca ccctccccct aagagtgacc
     5341 tctccggtca tggctacgct tttgttgacc ggtttccctg tcaatgcaga agcgatggca
     5401 gtggccatag tgattcccgc agaggggccg tccttgggga ttgccccggc agggacatgg
     5461 atgtgcaaat cagtgcgcga aaagaggtca tccttaatgc caagcccctt tgcccttgag
     5521 cgtatataac tcagggctgc ctgcgcagac tctttcatga catctcccaa ttgtccggtg
     5581 agggtaaggc cgcccttccc tttcatagta gtcgcttcaa catagatgat gtccccccct
     5641 gcctctgtcc aggcaagtcc ggtagagacg cccacctcgt ccttttccat ctcttcctca
     5701 ggaaggtact tgggcacacc gagaaacctg tggagattcc ggggagtgat aacaaaattt
     5761 ttctggcccc cttcggctat cctctttgcg acctttctgc agagattggc gatctccctc
     5821 tcaagattcc tcactcccgc ttctctcgta tattgcgata tcaccaggag gagtgctcca
     5881 tccgtcatct taagaatctt ttttgtgatt ccatgctcct ccagctgttt tgggacaaga
     5941 aagttcttcg cgattcccag cttctcctct gcggtatatc ccgacaagta gatgacctcc
     6001 atcctgtccc tcaagggacc ggggatcgtg tccacaagat ttcccgtagt gatgaacatc
     6061 acattggaga ggtcaaaggg gaccccgaga taatggtcaa caaaggcgtt gttctgctca
     6121 gggtcaagaa cctccagcaa ggcggaagag ggatcgcccc tgaagtccat tccgatcttg
     6181 tccacctcgt caagcataaa gaccggattg tttgttcctg cctgcttcat cccctgaatg
     6241 atccgtccgg ggagggctcc cacataggtc ctcctgtggc cgcggatttc tgcctcatcc
     6301 ctcacccctc caagagacat tctcacgaat tcccttccca aagaccttgc aatggattta
     6361 ccgagagagg tctttcctac tccgggaggt ccgataaaac agaggatggg acccttcatt
     6421 ttttccttta actttctcac gctgagatac tccaggatcc gttccttcac cttctcaagg
     6481 tcatagtggt ccttgttcag caccttctct gcggccttta tatcaagctt gtctttcgtg
     6541 gacctggacc acgggatttc caccatccat tcgagatacg ttctcactgt agccgcttcc
     6601 gcgctgtcag ggtgcatttt acccaggcgt ttcagttgtt tctctgcttc cttcagaacc
     6661 ttgtcaggca tcttggcttc ttctatcttc tttctcagtt cattgatttc ttctcccctt
     6721 tcgtcgatct cgccaagttc cttctgtatt gccttaagct gttctctcag gaaatactcc
     6781 ctctgtgtct tgtcgatctc accccttgcc tctgtctgta tcttctgctg cacaatgaga
     6841 agttctattt ccctgctcaa tatatcgctc acttttttca gcctctggac cggctctata
     6901 atctccagaa tctcctgcgc ctgctctgtt ttgagcccga gatttgaggc aaccagatcg
     6961 gcaagtctcc cgggctcctc gagattctcc acaaccacca taatgtcagg gaggatagtc
     7021 ttacccaggg aaaccgcctt gtcaagctgc tcctttaccg tcctcatgat ggcttctgtc
     7081 tcgatggtca tctcctccac cttttgatca gtgatctttt caattgtcgc ggtgtagaaa
     7141 ggctctgtct gggtaaatcc cagaaccctc gccttgctga ccccctgcac cagtatcttt
     7201 acccttccgt caggaagctt aagcatccgc atgatgatcc ctaccgtacc aatggcatag
     7261 aggtcttcct gggtcgggtt ttccatattc agatcccttt gagtgaccag catcaccata
     7321 cggtttgtgt tcaatgcctg atcaatggcc tttatcgaca tttccctccc cacaaagagt
     7381 gggagaatca tatagggaaa gaccacgata tcccttacag ggagcaccgg taatgtatcg
     7441 gggatctcta cttccttttc atccttctgt tctatctctg ccatagcatg ttttccccta
     7501 aaaagtccgc agtgccgggg aactcctcct cttcatttct ctatttttat cttgataacc
     7561 ctgtctttga tcttcggaaa ggtcacgatg attacgccgt cactatagga agcctttcct
     7621 gcaacggaat taacgggaac aggaatcttg accattcttc tgaaactctc aaatctccgt
     7681 tccatgcaga ggtatctgaa tcccttctct tcatctgttt tcctctttat tccctcaata
     7741 ataaggagat cctcatatac ctttatcagt atgtcctccg ggtctatccc cggaaggtcc
     7801 atctccagaa tgagactgtc atccgtttca tacaggtcga ggagagggag gtaccccgca
     7861 gtctctttgt gaaaatacag tccgctgcgc aaggacatgt cagccccctg cgccccggag
     7921 gacccctgct cccttcacac ccttgccacc ggtagtactc cgttccatgc tcattgcctt
     7981 ctcaaggata tatgccctaa agggctcaga cacctcaagg tttttcaacg caaagtccac
     8041 cgtagccttc agaaacccga ccttgtcccc tgcgtcgtat cgggtgcctg taatgggaaa
     8101 tccgtaaatg gaccttttcc tgagcaattg cctcagtgcg tcggtgagct ggatctcacc
     8161 gcctgcccct gccctttgtt tctcaaggat tttgaacacg tccggggtaa ggatatacct
     8221 tcctatgata gcaaggtttg agggagcatc ttcggccttt ggtttctcca ccaggtcttt
     8281 gatcctgtac acatccccct ctgctacgcc atcgataata ccgtatttag aaacctcagc
     8341 ctttggcacc gcttccagcg caatcaccgg acaatccacc tcttcatata ccctgatcat
     8401 ctctttcagg agggggtaat cgggatcgat cacatcatca ctcaagatca cggcaaaggc
     8461 ctcgtccttc acaaagggct tcgcacagag tattgcgtgg ccaagaccga gagccacccg
     8521 ctgccggata taggcaaagt taatatgact taacttattt atttcatcca aaagcttctt
     8581 cttgcccgca ttcttcagtt tttcttcaag ctcataggcg gaatcaaagt gatcctcaat
     8641 agcccgtttg tgtttccctg tgatgataat gaaatcctct attccgcagg atatggcttc
     8701 ctcaaccgca tactggatca taggcttatc aacaatggga agcatctctt tcggggacgc
     8761 ctttgtggca ggcaagaacc tcgtcccaag gcctgcagca ggcaagatcg cttttttaat
     8821 ccttttttct gtcatggtag tttcacttcg ctccccgtgt cacaatgatt tcctcaggac
     8881 gcttcgtagt cttcaaacca ggacctgcct gcaaaaaact gcttatctag ccctttccca
     8941 tggatttttc tcagcttttt tagtttattg gaaaagccct caatagtcaa gggactggat
     9001 ggtcttgcca tc
//
LOCUS       DATKOU010000145         3321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5195529, whole genome shotgun sequence.
ACCESSION   DATKOU010000145 DATKOU010000000
VERSION     DATKOU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5195529"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1422
                     /locus_tag="VMU21_06605"
     CDS             328..1422
                     /locus_tag="VMU21_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HUO77231.1"
                     /translation="MPFGSTISALTAILLGTALGFVLQRGRFCLNSAFRDIIFIQDLT
                     FFRAYLLCISVAIIGTNMLESSGLIFTYDQEAGHFVSTELMRQNFVPVANILGGLLFG
                     IGIVLAGGCASGIVYRLGEGQIGALIAVVGFFFGIGMTTDGMLSPVREYLKSFKVEVF
                     GVSNPAIWDLFGGGPTAKWGTIAVFTIVMLAFVFKGKPSFGKSGKGYAWGLTGVLVGL
                     LTILGWEVSSVFGGTPRGLAITTPLRELFNSMLTNSTHSPFREFSFIGIFTGTWGVFF
                     ILSVPFGSWISAVGLKEFKWKTPPAKEVITVFFGSILMGIGAVIAGGCNLGHGVTGMS
                     TMSLPSLVAIISIMLGNWVMVYFKFIRATE"
     gene            complement(1520..2263)
                     /locus_tag="VMU21_06610"
     CDS             complement(1520..2263)
                     /locus_tag="VMU21_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77232.1"
                     /translation="MKRHMERLETKKLRQRYPDSPWKWSTRWRSNRIEYSDKAEVIFS
                     YLTTAIIITGIALSFLLNTEGILKRFQENRMDSLIILYILLMGVLFSVRFAVISTNRW
                     RKFKRSSFVMFTFPGVIGGKLEGEIQTRCRRVPCSGFDLKLSCIEMDISFRKSIHDIT
                     ETVLWESKKKVQIDNIRMGPYGISFPVSFNIPANTEETDTVSRDRRIHWVLKAHGTGD
                     EHCLSAVFKVPVFRVRLEQLDNNLTSSRS"
     gene            3051..>3321
                     /locus_tag="VMU21_06615"
     CDS             3051..>3321
                     /locus_tag="VMU21_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77233.1"
                     /translation="MNKQKFEKVNHTAQAVQHAISAGENYPQLGALIQQLSSEIEALN
                     ARVTSGKERDLVQEYSKLLKMYQDGFLLWKYRTEFTRHNFVPKGRI"
BASE COUNT          855 a          750 c          818 g          898 t
ORIGIN      
        1 agatatagat agtcgggacg ttttacatgt caattcattg cgccattgga acgccgctga
       61 tatcagtcag cggcgttttt ctcatctctc acaattgttt ctttagtgcg tcattttccg
      121 acaaaggcgt tgacaatttg acggtagaca cgttaatatt ctacgattaa caagtaaaca
      181 ttctatgaaa tagtgttaac aggatcatta acagcctgtt aatttcctgt aaagaggagg
      241 aaaagggaac catggagaag ttttcggtat ttacatctcg agaaaacaaa aagaatattt
      301 taacgctcat gatggggagg aacgcttatg ccattcggta gtaccatttc agccttgaca
      361 gccatacttt tggggactgc gcttggattt gttctccaga gagggcgttt ctgcctcaat
      421 tcagcattca gggacatcat tttcattcag gacctcacgt tcttcagggc atatctgctt
      481 tgcatttcgg ttgcaataat aggtacgaac atgctggaaa gctctgggct catattcacc
      541 tatgatcagg aagcagggca ttttgtttca acggaactga tgcgtcagaa ttttgttccc
      601 gttgctaata tccttggcgg tctcctcttc ggtatcggaa tagtccttgc aggcggatgt
      661 gccagcggta ttgtttatag gttaggtgag ggccagatag gggctttaat cgctgtcgtc
      721 gggttcttct ttggtatcgg catgacaaca gacggaatgc tcagtcccgt ccgtgagtac
      781 ctgaaaagct ttaaagtcga ggtctttggg gttagtaacc ctgccatttg ggatctcttc
      841 ggaggcggcc cgacagcaaa atggggcaca atcgcagttt tcaccattgt aatgttggcc
      901 tttgtcttta agggaaagcc ttccttcggc aagtccggca agggctatgc atggggactg
      961 accggcgtcc ttgtcgggtt gttgaccata cttggctggg aggtatcttc tgtctttggg
     1021 ggcaccccga gagggcttgc cataaccact cccttgagag agttattcaa ttccatgctt
     1081 accaacagta ctcattctcc attccgggaa ttcagtttca ttgggatatt cacgggcaca
     1141 tggggtgttt tctttattct ttccgttcct tttggctctt ggataagtgc tgtaggcctt
     1201 aaggagttca agtggaagac gccgccggca aaggaggtaa tcaccgtttt ctttgggagt
     1261 atccttatgg gtataggcgc agtcatcgcc ggcggatgca acctcggaca tggtgttacg
     1321 gggatgtcca ccatgtcact tcctagtttg gtggcaatta tttcgatcat gctcggcaac
     1381 tgggtaatgg tctatttcaa gttcatcagg gcaacagaat aaatttaata tcagggcgat
     1441 aattggggct accttattga taagaaggtg gccctttttt tgtgaatgaa attgacatgt
     1501 gctgcattca ataagagggt cagctgcggg aggaagtgag attgttatca agttgctcaa
     1561 gtctcaccct gaaaacagga accttaaaga ctgcagaaag acagtgttcg tcgccagtgc
     1621 catgcgcttt tagcacccaa tgaatcctcc tgtcgcgtga tacagtgtca gtctcctccg
     1681 tgtttgccgg gatattgaag gagaccggga atgaaattcc gtagggtccc attcgaatgt
     1741 tgtcgatctg gactttcttc ttcgactccc aaagaaccgt ttccgttatg tcatggatgg
     1801 acttccgaaa agagatgtcc atttcaatgc atgagagttt caggtcgaat ccgctgcagg
     1861 gaacacgtct gcacctcgtc tggatctcac cctcaagctt tcctccaatg actcccggga
     1921 aggtgaacat cacgaaggaa gatctcttaa atttcctcca cctgttagtg gagatgacgg
     1981 caaaccttac ggaaaaaagc actcccatga gaagaatata aaggatgata agactgtcca
     2041 tccggttttc ttggaaacgt ttgagaatcc cctccgtatt caaaagaaag ctcagcgcta
     2101 ttccagtgat tataattgcc gtagtaagat aactgaagat aacctcggcc ttgtcggaat
     2161 attcaatcct gttgctccgc caccgcgtgc tccatttcca gggggaatcg ggatagcgct
     2221 gcctgagttt cttcgtctcg agccgctcca tgtgcctctt catagacaac tcatagagca
     2281 tattgctgcc ggtgccgact acgatgatgg gaaaaagaag ccagcgtgat cagcatccga
     2341 gattcagtcc tgccgacgag atagtcactt aagattgtcc atatgacgac gctgacaacg
     2401 agtattccgc cgctaaggat gaaccactta aagattctcg cataaaagac ccggttttcc
     2461 ctcttgcgaa tccacagagg ggagagcgct cctgcaaaca aaaggagcaa aagaaaactc
     2521 agcatgaaga cccaaaatgc atgcagcacg gcaaatgttc ctttcagtca ctcagagaat
     2581 caggtctctc ttgagtcctt tgcgtttttg acccgacaaa tctgctccta cataagtgtc
     2641 tccgtgtcct atgcagttca gtgtgccgga atcaaaggag gacgtattct tcattataac
     2701 aaagacagtg tttcctggta tattcccgct tcaggaaggg cctgccaggc aaaggaaagg
     2761 agaggagcac aggggaccgt gttgagccag ttgattgcac ccgtatgcag ctcttcacaa
     2821 ggatagcaaa gacaggatta agtttatcag ccgactacta ctgtacatgt ggtaaaatga
     2881 ccggggcagg tcacttgcgc aataagactc ccctgtagag gaggtgaaag gcaaatgaaa
     2941 ataacattag ccgacattag ttgaagaatt tccaaaagcc tatgctacaa gagggtattt
     3001 tttcagcggg gatatggctg ctccttgtct gtgccgcctg ttccccccaa atgaataaac
     3061 aaaagtttga gaaagtgaac cacactgccc aggcagttca gcatgccatc tcggctggtg
     3121 aaaactaccc gcaacttggt gcattgatcc agcagttgtc ttctgagatc gaagcgctga
     3181 atgcgcgtgt gacatccggg aaagagagag accttgtgca agagtattca aagctcctga
     3241 agatgtatca ggacgggttt ctcttgtgga aatatcgtac agaattcacc cgccataatt
     3301 tcgttcccaa agggcgtatc t
//
LOCUS       DATKOU010000146         7829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4815775, whole genome shotgun sequence.
ACCESSION   DATKOU010000146 DATKOU010000000
VERSION     DATKOU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4815775"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VMU21_06620"
     CDS             complement(<1..326)
                     /locus_tag="VMU21_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HUO77234.1"
                     /translation="MKRIGILLSALIMVLGLSVSVQATLEDMNDGTIYDTTTQLSWLK
                     NANTAGMMTWSQAVAWAATLNTGNGFAGLKGWRLPTTTQPDATCLDQGTPGGSFPLQG
                     HGQNCTG"
     gene            complement(728..1054)
                     /locus_tag="VMU21_06625"
     CDS             complement(728..1054)
                     /locus_tag="VMU21_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUO77235.1"
                     /translation="MKKCPYCAEEIQDAAIKCKHCGEFLDASSRPHRREDTTRWYFRK
                     SFIIIAVCCVGPLALPLIWWRPNTTRGWKIGLTIVILVLSWILLQLTLDSIQKITEYY
                     KLFETM"
     gene            complement(1157..2602)
                     /locus_tag="VMU21_06630"
     CDS             complement(1157..2602)
                     /locus_tag="VMU21_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO77236.1"
                     /translation="MAVYDFDIGILGGGAAGLTIASGAAQLGARTLLIDKEGRLGGDC
                     LHFGCVPSKTLIRTARVWHLANRAKEFGLPELEHKPVDFKAVASRIQSVISTIQKHDS
                     VERFCSLGAKVEFGQAMFVDEHSINLNGRIYSAKIWVIATGSSPSIPSIQGLDKTPFL
                     TNREIFSLDHLPRSMIFLGGGPVSTEMAQAFGRLGSRVTVVERGSHILGREDEDMAAS
                     VMKVLCSEGVTFHLNASVVSVRDLGTEREVVINKSEETTNLRAEAIVVAVGREANIKG
                     MGLKEIGMDFDKKGIRVDSRMRTSHKHIYAAGDVTGSYQFTHAAGYEGGIVVSNAIFH
                     LPKKADYTFLPWCTYTDPELASIGMNEAAAKAKKIAYSVWTEEFSSNDRSLAEGEEVG
                     RIKMVLDEHENPVGIQILGPHSGELLSEWVAVLNGKVKLSTLAAAVHPYPTLGEINKR
                     VVGTYFSGKIFSDKVKKALKFFFNLKGRACG"
     gene            complement(2619..3335)
                     /locus_tag="VMU21_06635"
     CDS             complement(2619..3335)
                     /locus_tag="VMU21_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HUO77237.1"
                     /translation="MKRDTLNKIAVLILILTIAIAFKLFHLGQYLTLAHIKESGQEYV
                     LLYKEHKALVIAGYMVIYIVATSLSLPGAVVLTIAGGGLFGLLTGTIIVSFASTIGAT
                     LACFVSRFFLRDWVEDKFSDKIGKVNEGIQKEGAFYLFSLRLIPIFPFWLINLVMGVT
                     KMRLVTFYWISQIGMLPATVVYVNAGKELTRIDSLKGIISPELLGSFALLGIFPLTAK
                     RIIALYKKRKSRSRPHNEYK"
     gene            complement(3364..4326)
                     /locus_tag="VMU21_06640"
     CDS             complement(3364..4326)
                     /locus_tag="VMU21_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HUO77238.1"
                     /translation="MSTKRILCADIGGTNSRFGLFEADPTGNLSVVETQWFRTDEVAS
                     FGHLIRLLRESEFQLKPGDADIVVIAVAGPVERGVYSAPPLIPWDVDLTDALTDYGFK
                     RSILINDFVAQAYATRSIVGQSAREILPGQIIPGGTVAVIGAGTGLGKAMLVSDSDGR
                     YIAIPSEGAHASFPFESQEEFRFQEFLIQELGEDYITANSVVSGKGLTLIHQFLTGEK
                     LEPKDVLAKCTLNCETLVWASRFYGRACRNFALETLSLGGMYITGGIAAKLPQLVTHD
                     AFSREFRSSKTMGRLLAQMPVTLMSDEESGLWGAAFLGQQVVQE"
     gene            complement(4390..4722)
                     /locus_tag="VMU21_06645"
     CDS             complement(4390..4722)
                     /locus_tag="VMU21_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HUO77239.1"
                     /translation="MTEYYRDEDLDRFGEIGGPKPDLFKKFMEWYTSALAPGTLSKRE
                     KALIGVAVAHAVQCPYCIDAYSKSCLEEGMSLEHITEAVHVAAAVRGGASLIHAIQAH
                     NSVEKISL"
     gene            complement(4784..5698)
                     /gene="arsS"
                     /locus_tag="VMU21_06650"
     CDS             complement(4784..5698)
                     /gene="arsS"
                     /locus_tag="VMU21_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HUO77240.1"
                     /translation="MDIQSAPLTPVDIALLQVNLGYRCNMACKHCHVEARPDRAEMMS
                     GDNVEAVIEVLKNNPIHTVDITGGAPEMSPHFTYLVERARLIGCHVIVRTNLTIFFEE
                     EMEYLPEFFSNHSVEVVASLPYYIDSDVDRVRGKGAFEKCIKALMKLNCLGYGSGVPD
                     KRLNLVYNPAGPFLSPAQNTLEEDFKRELRRRFEISFDRLYAFTNMPIGRFRNFLLRT
                     NGFDRYMDKLIQAFNPATLNGLMCRYLISVSWDGRLYDCDFNQMLGLTVHDTCQQHIK
                     EFDYTRLSGRTITVGNHCYACTAGQGST"
     gene            complement(5881..7545)
                     /gene="pgi"
                     /locus_tag="VMU21_06655"
     CDS             complement(5881..7545)
                     /gene="pgi"
                     /locus_tag="VMU21_06655"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HUO77241.1"
                     /translation="MLEKRNPLQTGAWKKLTAHYEYIKTHHMKDMFKEDPERFSKFSL
                     RFEDILVDYSKNIIKEETLLLLVELARQTGVKDAIEKMFSGERINETEHRAVLHVALR
                     NRSHSPFYVDRKDVMAETDAVLTQMEKFSSMVISGEWKGYTGRPMTDIVNIGIGGSDL
                     GPVMVTEALKPYCKPHIRTHFVSNIDGTHITETLKGLSPDTTLFMVASKTFTTQETMT
                     NAHTAREWFIKAAKDATFVKKHFVAISTNEAEVKKFGIAPENMFIFWDWVGGRYSLWS
                     AIGLSIACSIGFDRFVELLEGAYAMDRHFQDTPFERNIPVLLALIGVWYNNFFGAQTH
                     AILPYDQYMHRFPAYLQQGDMESNGKYLDRKGLEVHYQTGPVIWGEPGTNGQHAFYQL
                     IHQGTKLIPCDFIAPALSHNPVGNHHPILLSNFFAQTEALMKGKTHEEVVKEMKASGM
                     NEKEIRDLAPFKVFTGNRPTTSILLKRITPRALGSLIAMYEHKIFVQGVIWNIFSFDQ
                     WGVELGKQLAGKILHELADDATVASHDSSTNGLINVCKGMRKEVAG"
     gene            complement(7644..>7829)
                     /locus_tag="VMU21_06660"
     CDS             complement(7644..>7829)
                     /locus_tag="VMU21_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77242.1"
                     /translation="KPHLPYLLQDLCPPHGSLLLAIHYDRTIDVLQIRTFYLLATLLT
                     PHSCRITLHWVESGETE"
BASE COUNT         1901 a         2035 c         1753 g         2140 t
ORIGIN      
        1 ccggtgcaat tttgtccgtg cccttggaga ggaaagctgc cgccgggggt cccttgatcc
       61 aaacaggtcg cgtccggttg agtggttgtc ggaagccgcc agccttttaa gcccgcaaat
      121 ccattaccgg tgttcaaagt cgctgcccag gcaaccgcct gactccaagt catcatccct
      181 gccgtattgg cattcttgag ccagcttagc tgcgtggttg tgtcatagat cgtcccatcg
      241 ttcatgtcct caagcgttgc ctgaacagac acagacaacc caagcaccat aatcaaagca
      301 cttaataaga tgcctatcct tttcattgtt cttgtcctcg ccttcctata ttgataaaac
      361 tgtagtcacg ctattacttt atcatattct atttccgtcg caagtatctt aagaaagtac
      421 gctggagaga taacgcaatt tttgtacctt tggttattga tctcgcaatt aataaagccc
      481 acaatatcta aaatgcctac gacatggctt tattaaggca gtgagtgtag tataaaaact
      541 ctcggttatc agatattttt ttctgtcttt aacgcctggt gaaacgccaa acctattgcc
      601 atttcacgat cagaaggtac aattaagtca cccaacgaat tgattcgaca tggtttcccg
      661 tgtgggattt gaaccagggt ccttccagga agaaaccttt ttctttagtc tcctctttct
      721 ccctttgcta catcgtctca aacagcttgt aatactctgt tatcttttga atcgagtcca
      781 gagttaattg caacaatatc caacttagca caagaattac aattgtgaga ccgattttcc
      841 aacctcgcgt tgtattagga cgccaccaga ttagcggcaa cgccagaggt cctacacaac
      901 agactgcgat gataataaaa gattttcgga aataccacct ggtcgtgtct tctctacggt
      961 gtgggcggga ggaggcatcg agaaactccc cgcagtgttt acactttatt gccgcgtctt
     1021 gaatttcttc agcacaataa ggacacttct tcataattca tttcttttgg cagaatatcg
     1081 gaatcatgtc catcacccgc aattgccgtt tatacccttc accattttag cagagaaggc
     1141 ggataaatgt ttatggttat ccgcaggccc ttcccttaag gttgaagaaa aacttcagag
     1201 ccttcttcac tttgtcagag aatatcttgc ccgagaagta tgttcccaca accctcttat
     1261 ttatctctcc aagggttgga taagggtgga ccgctgccgc aagggttgaa agcttcactt
     1321 tcccgttaag gaccgctacc cattcactga gcaattctcc tgagtgaggt cccaggatct
     1381 gtatgccaac agggttttcg tgttcgtcca gaaccatctt tatcctgccc acctcttcgc
     1441 cttccgcaag gcttctgtca ttagaactga actcctctgt ccagactgaa tacgcaatct
     1501 tctttgcctt tgctgccgcc tcattcattc cgatacttgc gagttccgga tcagtatagg
     1561 tacaccaggg aaggaatgtg tagtcagcct ttttgggcaa atgaaagatg gcattactca
     1621 ccacaatacc tccctcatac cctgcagcat gggtgaactg gtaagagccg gtgacatctc
     1681 cggcagcgta aatatgtttg tggcttgtcc tcatccggct gtccactctt atcccctttt
     1741 tgtcaaagtc catccctatc tctttaagtc ccatgccttt gatatttgcc tcccttccca
     1801 ctgccactac gatagcctct gccctgagat tggttgtctc ttcgctctta ttgataacca
     1861 cctctctttc tgtaccaagg tctcttacac tcaccacgga cgcattcagg tgaaaagtca
     1921 ccccctcgct gcataagacc ttcatcacgc tggcggccat gtcctcatct tccctgccga
     1981 ggatgtggct tccgcgctct acaaccgtca ctctgcttcc cagacgtccg aaggcctgag
     2041 ccatctcggt ggatacaggc cctcccccaa gaaaaatcat ggagcgggga aggtgatcca
     2101 atgaaaatat ctccctgttg gtaagaaaag gtgttttatc caggccttgg atagagggaa
     2161 tggagggaga ggaaccggtt gcaatgaccc agatctttgc agaatagatt cttccattca
     2221 gattgattga atgctcgtca acaaacatcg cctgaccgaa ttcaactttt gctccgaggc
     2281 tgcagaaccg ttccacagaa tcatgcttct gtatagtact gatgaccgat tgtatccttg
     2341 atgccacagc cttaaaatca accggcttgt gttcaagctc aggaagccca aactcttttg
     2401 ccctgttcgc cagatgccat acccgggcag ttcttataag tgtcttgctc ggcacacacc
     2461 cgaaatggag acagtctcct cccagcctgc cctctttatc gatgagaagg gttctggcac
     2521 ctaattgggc agcccctgaa gcgatagtga gccccgctgc tccacctccc agtatgccga
     2581 tatcaaaatc atacactgcc atgatgacct ccctgctctt atttatactc gttgtgcggc
     2641 cgggatctgc ttttcctctt tttgtataag gcaatgattc gttttgcagt caagggaaat
     2701 atgcccagaa gtgcgaatga accgagcagt tccggggata taatgccttt cagagaatca
     2761 attcttgtca gctcctttcc tgcattaaca tacacaaccg tggcgggaag cattccgatt
     2821 tgggaaatcc agtaaaaggt cacaaggcgc atcttcgtga cccccatcac aaggttgata
     2881 agccagaaag gaaagatggg gataagtcgc aggctgaaga ggtaaaaggc cccttctttt
     2941 tggatcccct cattcacctt ccctattttg tccgaaaatt tgtcttcaac ccagtcgcgg
     3001 agaaaaaaac gtgacacaaa gcaggccaac gttgcaccga tggtgctggc aaaagataca
     3061 atgatcgtac cggtcaatag cccgaaaagg cctcctccgg caatagtcaa tacaactgcc
     3121 ccgggcagag acagtgaggt cgcaacaata taaatcacca tgtacccggc aatcacgagg
     3181 gccttatgct ctttatataa caacacatat tcctgcccgg attcttttat atgtgcaagg
     3241 gtcagatatt ggccgaggtg aaataacttg aaggctatgg caatagtcaa tatgaggatg
     3301 aggaccgcta tcttattcag cgtatctctt ttcaaaggga accccagtgc cgatgattcc
     3361 ccatcattcc tggactacct gctgtcccaa aaaggctgcc ccccatagac cactctcttc
     3421 gtcagacatg agtgtaacag gcatctgagc cagtaatctc cccatggtct ttgaactccg
     3481 aaactcccgg gaaaaggcat catgagtaac gagctgtgga agttttgccg ctattccgcc
     3541 tgtgatatac attccaccaa gcgaaagggt ctccaatgca aagtttctgc acgcccttcc
     3601 gtagaaacgg gatgcccaga caagggtctc gcaattcagc gtgcattttg ccaagacgtc
     3661 ctttggctca agtttttccc ctgtgagaaa ttggtggata agagtcaggc cctttccgga
     3721 gacaacgctg tttgcagtaa tatagtcttc tccaagctcc tgaataagga attcctgaaa
     3781 tctaaattcc tcctgagact cgaagggaaa gctggcatga gccccctcag agggtatcgc
     3841 gatatacctg ccatcgctgt cagaaacgag catggccttg ccaaggccgg tgcctgcacc
     3901 aatcaccgca actgtccccc cgggaattat ctgcccgggc aatatctccc gagcagattg
     3961 tcccacaatg gatcttgtag cgtatgcctg ggcaacaaag tcattgatca aaatggatct
     4021 cttaaatccg tagtcggtca aggcatcggt aaggtcgaca tcccatggaa tgaggggagg
     4081 agcactgtaa acacctcttt ccacagggcc tgccacagcg atgacgacta tgtcagcatc
     4141 tccgggtttt agctgaaatt cactctccct gagcagcctg atcaggtgcc cgaatgatgc
     4201 aacctcatct gtcctgaacc attgtgtttc gaccacagag aggttcccgg tggggtctgc
     4261 ttcaaagagg ccgaatctgc tgtttgtccc gccgatatct gcgcagagaa tccttttcgt
     4321 gctcacgtgg tataggctcc tctctttgga ttgaacaccc tgacagtctt tcccaggctc
     4381 ttgcattatc tagagcgaaa tcttttcaac ggagttatgt gcctggatcg catggataag
     4441 ggaggctcca ccgcggacgg cggcggccac atgcaccgcc tccgtaatat gttcaaggct
     4501 cattccctct tccaggcagc ttttggaata ggcatctatg caataggggc attggacggc
     4561 atgggcaacc gcaactccta tgagcgcctt ttctcgtttg ctgagagttc cgggtgccag
     4621 ggctgaggta taccattcca tgaatttctt aaagagatca ggcttaggtc cccctatctc
     4681 accgaacctg tcaaggtctt catctctgta atattcagtc atcgcattcc tccctcattc
     4741 attatgtaat acaggcaact gcctgctagg tgactgcgcc cactcaggtc gaaccttgtc
     4801 ctgcagtaca tgcgtagcag tggttgccga ctgttattgt tcttccggaa aggcgcgtat
     4861 agtcaaattc cttaatatgc tgctggcatg tatcatggac ggtgaggccg agcatctggt
     4921 tgaaatcgca gtcatacagt ctgccgtccc aggatacact aatcaggtac cggcacatca
     4981 gaccgttaag ggttgcaggg ttgaaggcct gtataagctt gtccatgtac ctgtcaaaac
     5041 cattagttct gagcaagaaa ttcctaaatc tgcctatagg catgttcgtg aatgcataga
     5101 gtcggtcaaa tgaaatctcg aaccgtctgc gcaactccct tttaaaatct tcttccaacg
     5161 tgttctgtgc aggggagaga aaggggcccg caggattgta tacaaggttt aaccttttgt
     5221 ccggcacgcc acttccatag ccaaggcagt ttaatttcat gagagccttt atgcacttct
     5281 cgaaggcgcc ttttcccctt actctgtcaa cgtcgctgtc tatataatag ggtaaagatg
     5341 caacaacctc cactgagtgg ttgctgaaaa actccggcag atactccatc tcctcctcaa
     5401 aaaatattgt aaggtttgtc cggactatga catggcaacc gatcagtctt gctctctcca
     5461 caagataggt gaaatgagga ctcatttcag gcgcaccgcc ggtgatatca accgtatgaa
     5521 tggggttgtt cttcaatacc tcaatgactg cctcaacgtt gtctccagac atcatttcag
     5581 ccctgtcagg gcgtgcctcc acatgacaat gtttgcaggc catgttgcat ctatagccaa
     5641 ggttgacctg gaggagcgcg atatctaccg gcgtcagggg tgctgactga atatccatta
     5701 tcctgtcttg aaagtgtgtg gtattgatac tcacctcctt cgtattgccc tttcttcaag
     5761 tcctcttcat ctctgtctta ccgtaaccaa cctgctctta ctatcaatga tacatatttc
     5821 ccggtataca tgaaagtaat accgggaaat atgcaatgct ggtctcctgc caaacctgca
     5881 ttaccctgca acctcttttc tcatgccctt gcatacattg atcaacccat tcgtggacga
     5941 atcatgggat gccacagtgg cgtcatccgc gagctcatgg agaatcttgc ctgcgagctg
     6001 tttcccgagt tcaacccccc actggtcaaa gctgaagata ttccagatca ccccctgcac
     6061 gaaaatctta tgctcataca ttgcgatgag gctgccaagt gcccttgggg taattctctt
     6121 taggagaata gaggttgtcg gccgattgcc tgtgaatact ttaaaagggg caaggtccct
     6181 gatttccttc tcattcattc ccgaagcctt catttccttg actacctctt catgggtctt
     6241 ccctttcatc aatgcctcgg tctgggcaaa gaagtttgag agcagtatgg ggtgatgatt
     6301 tcccacagga ttatggctca atgcaggcgc aatgaagtcg cagggaataa gttttgtccc
     6361 ttgatggata agctggtaaa atgcatgctg accgtttgtc ccgggttctc cccagatcac
     6421 ggggcctgtc tgatagtgca cctcaagacc ttttctatct aagtatttcc cgttgctttc
     6481 catatctccc tgctgcaggt atgcgggaaa cctgtgcatg tactggtcgt agggcaagat
     6541 cgcatgggtc tgggcaccaa agaagttgtt gtaccagact cctatgagtg caaggagtac
     6601 aggaatattt ctctcaaaag gggtgtcctg aaagtgcctg tccatggcgt acgccccttc
     6661 gagaagttca acaaagcggt cgaatcctat ggaacaggcg atcgagagtc cgattgctga
     6721 ccagagagag tatctgcccc ctacccagtc ccaaaagatg aacatattct cgggtgctat
     6781 tccgaacttt tttacctcag cttcgttggt cgatatagct acaaaatgtt ttttgacaaa
     6841 cgtggcgtcc tttgccgcct ttataaacca ttccctggca gtgtgggcgt ttgtcatagt
     6901 ctcctgggtg gtgaaggttt tggatgcaac cataaacagt gttgtgtcgg gtgaaaggcc
     6961 ttttagggtt tccgtgatat gggttccgtc tatattggaa acaaagtggg ttctaatatg
     7021 cggcttgcaa tatggcttca gtgcctcggt taccataaca ggacccaaat cagagccgcc
     7081 gatgcctatg ttgacaatat ccgtcatggg tcttccggta taccccttcc attcccctga
     7141 aatgaccatg gaagaaaact tttccatttg cgttaagacc gcgtctgttt cggccattac
     7201 gtctttccta tccacataga aaggactgtg cgagcggttc ctgagcgcca catggagcac
     7261 tgcccggtgc tctgtctcat tgatcctttc accagaaaac attttttcta tggcatcttt
     7321 caccccggtc tgccgggcca gctccacaag caggagaagg gtctcctctt ttatgatgtt
     7381 ttttgagtaa tccacgagaa tgtcctcaaa ccgtaaagag aatttggaga agcgttccgg
     7441 atcttccttg aacatgtcct tcatgtgatg ggttttgata tattcatagt gtgcagtcag
     7501 ttttttccat gcacctgtct gtaaaggatt tctcttttcg agcataatgc ctccctctat
     7561 gcgcactcat tcagttacca gtcattatgt gtatccagcc tttcttgctt gatacacgcc
     7621 attcatatgc tgataagctg atatcactcc gtctcaccag attctaccca atgcaaagtg
     7681 atcctgcaag agtgcggtgt gaggagtgta gcgagcaaat aaaatgtccg tatctgtagc
     7741 acatcaattg tccggtcata atggatggca aggaggagcg atccatgagg cggacagagg
     7801 tcctgcagga ggtacggaag atgcggttt
//
LOCUS       DATKOU010000147         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_194346, whole genome shotgun sequence.
ACCESSION   DATKOU010000147 DATKOU010000000
VERSION     DATKOU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194346"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /gene="gspD"
                     /locus_tag="VMU21_06665"
     CDS             complement(<1..1792)
                     /gene="gspD"
                     /locus_tag="VMU21_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HUO77243.1"
                     /translation="MRTRAIIILCLALAVVLLIPPPVIGESKKPEKVTFNFVDVELPA
                     ITKFISDITKKNFVFDERVKGKITIIAPSKLSVDEAYSLFTSVLELKGFTVVPSGVDV
                     YKIIPSAEAKQKGIELSSDRQTVNESYIARLISLKHISSDDALKFLQPIVSKDGYVSA
                     FGPGNLLLVIDSGLNMEKILSIVEIIDQPSMREGPELIFLKYCSADAVAKMLNEGMSR
                     RARGAPQQVIAEETKVVADQRLNAVVLFGDKGARESMKSLVEMVDVPSREAQGRINVY
                     FLENADATELAKILEGIIRGTQTPRQAAPGAPQVTPFEAVGGITISADKASNSLVIVA
                     SPSDYQNLYQVIKQLDKRRRQVYVEAMLIEASVNKLRDLGVKWRASATEGGKPVVIGG
                     FGTIDNAAIQGIVQGLAGATLGGLGNFLTVPVTTTDTTTGQPTATVLSIPGFAVLFNL
                     NEFRDVVNVLSTPQILTSDNKEAEILVGQNVPFISQSSTTTGLGVTTTGSSVQGVVNS
                     IVRQDVGIILKITPQITEGDHVKLDIYLENSSVVNQSTTITVTVGPTINKRSTKTSVV
                     VKDNQIVVVGGLLQDTSEDQITKVPLLGDIP"
     gene            complement(1804..2673)
                     /locus_tag="VMU21_06670"
     CDS             complement(1804..2673)
                     /locus_tag="VMU21_06670"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HUO77244.1"
                     /translation="MYTSDLLYRYPRLPYIINLLLSLVLIIFLLVFVRDILSGISKKG
                     EKAPAPPRAPVHQVKRVLQEYAGILKNNPFGFAGGQLKELSVAKEGSVSQSDITLIGT
                     VSGSARNSYAIFVDKSGKQELVKVGEPVFGMGKLKRVDSDRAYISEGGREVKIPMAEI
                     LIIKEVNPPSGSRMASDLARSVGRGMFLVDQKKILNALENPNQLMTDARLQPNLVDGK
                     QEGYILREVRNGGIYQSLGLQNGDALLRINEYNISNPENALQAFTALRGMDRVQVDIV
                     RDGTKMTLTYQIR"
     gene            2901..>3172
                     /locus_tag="VMU21_06675"
     CDS             2901..>3172
                     /locus_tag="VMU21_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HUO77245.1"
                     /translation="MIKTFFLNLSLNKKLVLMMLFLSSILLSVLFFLYWQSEKVLLTE
                     LGNQTAELSKAIQVGVEEVTGSGSTDESRLAQYLKNLNSKGINEIS"
BASE COUNT          744 a          882 c          738 g          808 t
ORIGIN      
        1 gcgggatatc cccgagcaac ggcacctttg ttatctgatc ttcactggtg tcctggagca
       61 acccgccgac gacgactatt tggttatcct tcaccaccac cgaggttttg gtggagcgct
      121 tattgatggt ggggcccacg gtgaccgtga tcgtagtgga ctgattcacc acagatgaat
      181 tctccaggta aatgtctagc ttgacatgat ctccttcggt aatctggggc gtaatcttca
      241 atatgatgcc gacatcctgc ctcacaatgg aattgacaac cccctgcacc gaggaaccgg
      301 tcgtggtgac gcctaagcct gtggttgttg agctttgtga tataaacggc acattctgcc
      361 ctacgagaat ttcagcctcc ttgttgtccg aggtgagtat ctggggagtc gaaaggacat
      421 tcaccacatc cctgaactcg ttcaggttga agaggaccgc aaagccggga attgaaagca
      481 ctgtcgcggt cggctggcca gtcgtggtat cagtcgtcgt tacagggaca gtcaggaaat
      541 ttcccaaccc accgagggtg gcgcctgcga gcccctggac tatgccctga atagccgcat
      601 tgtctattgt gccaaaaccc cctatcacca caggttttcc cccttctgtc gccgatgccc
      661 tccatttcac gcccaggtcc ctcagcttat ttacggacgc ctcaataagc atcgcctcaa
      721 cgtatacctg tctccttctc ttgtcgagct gcttgatgac ctgatagaga ttctgataat
      781 cagaaggcga tgcaacaatg acaagcgagt ttgacgcctt gtcagcactg atcgtaatcc
      841 ccccgactgc ctcaaaggga gtcacctgcg gagcgccagg agcagcctga cgcggcgtct
      901 gggtacccct gataatcccc tcaaggatct ttgccagttc ggttgcgtca gcgttttcca
      961 ggaaatagac attgatcctt ccctgtgctt cacgagaggg gacatcaacc atctcgacaa
     1021 gggatttcat agattcccgc gctcctttat ccccgaagag gactactgca ttcagcctct
     1081 ggtcagccac cacctttgtc tcttctgcaa tgacctgctg gggagcaccc cgcgccctcc
     1141 tgctcatccc ctcattgagc atcttcgcca ctgcatcggc gctgcaatac ttaaggaaga
     1201 taagctcagg tccctccctc atggagggct ggtcaatgat ctccacaata gagagtatct
     1261 tctccatgtt caaccctgaa tcaatcacca ggagaaggtt cccgggtcca aacgcagaca
     1321 cataaccgtc cttagacaca atgggctgga ggaacttaag tgcatcatcg gacgatatat
     1381 gttttagtga aataagacgg gcaatatagc tctcattcac cgtctgtctg tcactggaga
     1441 gttctattcc cttttgcttt gcctcagcag aagggatgat cttgtaaaca tcaacgcccg
     1501 aaggcacgac agtgaacccc ttcaattcga gcacagaggt aaacaggctg tatgcctcat
     1561 ccacactcag cttggagggt gcgatgatgg taatcttccc ctttacccgc tcatcaaaaa
     1621 caaaattctt ctttgtgata tcgcttatga actttgtaat ggcggggagc tcaacatcca
     1681 caaagttgaa ggtcactttt tccggctttt ttgactcccc gatcacagga gggggaatga
     1741 gcagcaccac cgcgagggca aggcacagga ttataatcgc acgtgttctc atctcatctc
     1801 tcattatcgg atctggtacg tcagcgtcat cttcgttccg tccctcacaa tatcaacctg
     1861 caccctgtcc atgccccgca atgcggtgaa tgcctggagt gcgttctcgg gatttgagat
     1921 attgtattca ttaatcctca gaagggcgtc gccattctga agtcccagac tctgatagat
     1981 tccgccgttt ctcacctcac ggagaatata accctcctgt tttccatcca cgagattcgg
     2041 ctggagcctc gcatcggtca tgagctggtt ggggttttcc agggcattga ggatcttctt
     2101 ttggtccaca agaaacatcc ctcttcccac gctcctcgcg aggtccgatg ccatgcggct
     2161 gcccgaagga ggattgacct cctttatgat gagtatctct gccatgggga tctttacctc
     2221 cctgcccccc tcgctgatat atgccctgtc actgtccacc cgcttcagct tccccatacc
     2281 gaaaaccggc tcgccgacct tcaccagttc ttgttttccg ctcttatcca caaatattgc
     2341 atagctgttc ctggcggaac cggagaccgt tcctatgagg gtgatatccg actgggagac
     2401 tgagccttct ttcgcaacag agagttcctt cagctggccc ccggcgaaac caaatgggtt
     2461 gttcttcagt atgcccgcat attcctggag aaccctcttc acctgatgca ccggagcccg
     2521 gggaggggca ggggccttct ctcccttttt tgagatgccc gagaggatgt ccctcacgaa
     2581 aacgagcaga aagatgatca atacaaggga caggagcaga ttaatgatat agggcagtct
     2641 tggataccta tatagtaaat cgctcgtgta catacacaaa cctaaagaat tataaacaaa
     2701 tgaatggaag ttttacaata aagaacaggc tcgatccgtg tgttcgggaa ttgcaggacc
     2761 ccaccccatg gacagcgtcc ctttcccttt tccattcctt ttacgctata ataagcttaa
     2821 agggatcttg tcccgcgaga tttcccggac aacggatcat tcatatgcgg gatctgacag
     2881 ggtaaggcac ttgaaattgc atgattaaga ccttcttcct aaatctttcg ctgaacaaaa
     2941 aactcgtcct catgatgctc ttcctgagtt ctattctgct ttccgtcctg tttttcctct
     3001 attggcaatc cgaaaaagtg ctcctgaccg aactcggaaa tcaaacggct gagctctcca
     3061 aggccatcca ggtgggggtg gaagaggtta cggggagtgg ttcaacagat gaatctcgtc
     3121 tcgcacagta cctgaaaaat ctcaattcaa aagggatcaa tgagatctcg at
//
LOCUS       DATKOU010000148        17962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4817529, whole genome shotgun sequence.
ACCESSION   DATKOU010000148 DATKOU010000000
VERSION     DATKOU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 17962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17962
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4817529"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VMU21_06680"
     CDS             complement(<1..192)
                     /locus_tag="VMU21_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77246.1"
                     /translation="MWREFAAECGQCGRCKDEGLLDPEAFPVLMKAPPRTIDILFVLE
                     APNRGDTYDPRKKYLTVEPN"
     gene            674..1456
                     /locus_tag="VMU21_06685"
     CDS             674..1456
                     /locus_tag="VMU21_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO77247.1"
                     /translation="MGHDLWLSLFGFLVGMFGTLIGAGGGFILMPVLLLLYPHDSPET
                     ITSISLSVTFANAFSGTMAYARMKRIHYTYGLIFAAAAIPGAISGALVTYYVHRRTFD
                     FIFAILLIIASVILFLKTPMGRGSDAHRSVQLSPRYFRLGILISAAVGFVSSFLGIGG
                     GIIHVPALAGILGFPVHIATATSHFILAIVTFAGVMVHIITGTFQHGIVRAIFLAFGV
                     LFGAQAGALFSSKVEARIIIRLLAVALALVGIRLLLLSLNEF"
     gene            1838..2257
                     /locus_tag="VMU21_06690"
     CDS             1838..2257
                     /locus_tag="VMU21_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77248.1"
                     /translation="MKGYRKIVIAVNGSKDVLTQGLRLAQDEKCWVTVVKVVPPHNGD
                     LNLVGIRNIGDVLDNGANNERNEIMNIAEAESALVKTRVEEGDIPEKIVEVAEDEKCD
                     LIIMGTNKTSWFKKLFGKNVVERVISQAPCPVLVVGA"
     gene            2269..2760
                     /locus_tag="VMU21_06695"
     CDS             2269..2760
                     /locus_tag="VMU21_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77249.1"
                     /translation="MREKQLLFVTYHDENLDEGLSYALDLAKTMNDGIEILVVYKRKV
                     LEKFEDMMTAITFAESDMHKTAKEMISADYEKNNEDYEKRIALLEEQCRKAGIRVSIS
                     TAATDAVSAVKNILRQNVRIDMVLLSPSITNGGNVTSKVINKLVKTASRPIVTMAKNI
                     HAA"
     gene            2781..3698
                     /locus_tag="VMU21_06700"
     CDS             2781..3698
                     /locus_tag="VMU21_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO77250.1"
                     /translation="MHLYLPVALTSINVLIPVGLGLAVGLLSGLFGVGGGFLMTPLLI
                     MFGIPSTVAAATDSNQIVAASTSGTYAHWRVGNVDFKMGFYLLVGGFTGGLFGVQAIK
                     VLNAMGNADFVIKMTYVFMLGIVGIYMFIESLASMKKKQPETVAPAKESRMTRFLKSL
                     PFQTHFEKSGVSHSTLLPIFFGGLVGILAAIMGVGGGFLMVPVMVYILRMPMHVVVGT
                     SLFQILFNCIEVTFLQAYTNHTVDFILAVLLLLGSTVGAQVGAVFGRKLKGEQLKIIL
                     AVIVLVVTVKIVFDLTLSPSLLLAQGGGH"
     gene            3700..4500
                     /locus_tag="VMU21_06705"
     CDS             3700..4500
                     /locus_tag="VMU21_06705"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HUO77251.1"
                     /translation="MKVTILKSGLKNTIFLGVALMVLVLGAAGNASAELTATANHDHI
                     NVDFFYHGSSVSVRGTADQGIDLVIKITSPEGHQELLKKGKLAGFLWMNVGKLTFEKT
                     PKFYGIHSTKRLEDILSRDEMDKYALGYPALERHVEISPVGSEGEKSDWFNQFVKFKE
                     SSKLYSVSSGKINMTAQGGKQNYYVLLDWPYQALPDHYTISVYAVKDKKVIETAETHV
                     LVEQVGTVKSLAGMAKNNGSLYGIISILAALGAGFGVGMVFRKGGGAH"
     gene            4546..5436
                     /locus_tag="VMU21_06710"
     CDS             4546..5436
                     /locus_tag="VMU21_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77252.1"
                     /translation="MLIHTDSAVKTMYETILVGFDESDSSKAALSEAAHWIKRHGGKI
                     ILVHAVYFDEEEFGIAPDQLDRRLKVGEKICVQAKESLATGLGIEVNSLLCEGDPPEV
                     IQDVAKGKKADLIVLGTYGRKGLKRLLMGSVTSQVIVNSPTDVLVVKKPCTECSGTYR
                     SILVPFDGSVSSERALARACHLSKIDNGEITALYVIPRYEEMVEFFRTTSIQKSLHLE
                     AQKIIHKAKELASGENVSIKTLIDEGNAGEKIIETAGLNSSDLVVMGASGWRGVNRAI
                     MGSSTERVIIHATCPVLVVR"
     gene            5585..5728
                     /locus_tag="VMU21_06715"
     CDS             5585..5728
                     /locus_tag="VMU21_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUO77253.1"
                     /translation="MHLIVIPAIGLVVFGPGKLPQIGADLGKSIREFKKAISDKAEDL
                     NPS"
     gene            5971..7602
                     /locus_tag="VMU21_06720"
     CDS             5971..7602
                     /locus_tag="VMU21_06720"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HUO77254.1"
                     /translation="MVEYKFMRLVRRGLLTKEELDNAILESDSSKKYIEDILLKKGIP
                     RHEILLCISEFYNCPFVEYDEDITVSRGVLRLVDPLSLKNELWIPLSVFNRKAGVIAY
                     RPEDRALAEKIREMLGADEIEFRAGLPNDIIRIIENNFDLNPDFPPAAGRTPLAKVRT
                     YLAERRSLLAEQRTSLARGRTGLAFLRTGLAFIAIAVTFFRVFGAGYLSIIELLLFLG
                     GVAAVFDGLKWYMPVRKKAGKVLDYISQQSPPGFTCLEVSNPGEDPVFTRSEVVESAE
                     DLRAGWGKLSPVERRRFLANDRTDLAEERTILASVRTRMAKARTGLAFGRTGIAFSGL
                     GIALLRQFRESSWTFFDIALILAGALMTLEGVIWYFSGRRAGKAGLKAVLKAEEQMSI
                     WESAFPPFYRSSPVCFPPVKACHAPGVWATTGLALERTVLAERRNIMARLRTIMARSR
                     TGMAFIRTGVSLSAIGAGLLIYFGASNLAWTAFDMALIFAGIVLIADGVHWYLPAEKT
                     KGQFPYCYGDLEIRIPDYSVPGRSWKKAVFSHDDI"
     gene            7589..8791
                     /locus_tag="VMU21_06725"
     CDS             7589..8791
                     /locus_tag="VMU21_06725"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77255.1"
                     /translation="MMTSKSGLRFGHLVEEGLLTEKEINLAYDASMARGVNLERILLQ
                     EFGVPRLSLLEALSRHYDCPYIEYDERLPVPTEVFEGLNSDVLSRSLWFPLIKEEGAV
                     TIAANDPRSPRVSEEARAHFPEVRLEFRVALASDIQWYIRDFLHAKPKFLIGIERTGL
                     AFWRNTMAHWRTRLACYRTDLAKGRTSLAILRWGLGLIAFSDVLLRTRKDLFMITYAY
                     WAMMLAGLTFSIFGFAKYLQVRRSRISPPKHHTLVEVTAATTMFLENYHLEGASAQKR
                     TKGTMLARLSDCLPNYCTILNPVPASKERTHLARERNVLAAQRTIAACYRTMYSRART
                     GLAFIRTGVAFFSLGLGFLEYFLFGLFSIIDSSLMIAGLLMVADGVLWYMPARKEQSE
                     LPRCPVPQ"
     gene            8808..10223
                     /locus_tag="VMU21_06730"
     CDS             8808..10223
                     /locus_tag="VMU21_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77256.1"
                     /translation="MKIEHRIILSNVFNVALIVLIGLFAIQNLNLMLTKLRFVEIADD
                     LNASFLEMRLSEKNYFLYKDESALSGIKEKIEKTMESVEAVGDDISRAVGENNLNQLL
                     AYLKNYSGIVEELRRGSRDVQLETRLRNAGRKLKEFSETMTVLEKKQVNDIIARSKKV
                     LFSSFWAILISAIAVSHFISQRILRSLREIQRLAISISEGNFRTIEHIPSRDEFGSLM
                     KAINFMSEELKNREEEIIQSKKLASLGVLTAGVAHELTNPLNNISMIAQTYEEVYDGL
                     SNEQRIEFMNKVERETERIKEIVKNLLDFSKPKEVVLKEADINTAIQKTLKLVQNMLD
                     ISNVETKIVLEDSLPPVFTDEHQFQQVLVNLITNAVHAMPGGGRLFLGTRARRQRDTI
                     EVSVMDTGKGIPPEHLPHIFDPFFSTKGEGGTGLGLSVSYGIIKHHGGTIRVESKVGV
                     GTTFTIELPAYKKKEENNGSL"
     gene            10210..10575
                     /locus_tag="VMU21_06735"
     CDS             10210..10575
                     /locus_tag="VMU21_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77257.1"
                     /translation="MDRYRIMVIDDEKIVGDMAKISLEQDGYEVETFLNPAPALSRLK
                     EMKFDVVVTDYKMKGIDGMEVLKTVKALYPETKVIMITAFANLDTAIEALRGDVHDFF
                     PKPVKIRELKESIRKALSK"
     gene            10591..13143
                     /locus_tag="VMU21_06740"
     CDS             10591..13143
                     /locus_tag="VMU21_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HUO77258.1"
                     /translation="MKNLSVILKQAFGKRSRGSPVDMRTFLHNFKTALESNNRALEII
                     TDMGEKLSGEYLFDINYIRKAYAELHANIRSSITTFNSLTRNRYEIGPACDRIDSMII
                     RVIYEEAPFQAGRVVFYEGITWEMAREVGGKNYHLSQLRKDLDLNVPGGFTITALTYD
                     EFIRHNGIDKKIEELSSRPYGDKDLQDIRDAIIAGEFPEPAGSEIEKATDKLKDPGRS
                     NEFLAVRSSAEEEDGDFSFAGQFETILNVPLESFAVKAAYKKVISSLFAPTAVSYQKR
                     LGYAIGGMRMPVGCVLMVDAVASGVVYTSDPSGGRNRLTIIAAWGLGKSVVEGKVEPD
                     VYSLEKGPALKLIGTRVGTKETMVINSSSGGTVEMKTPADLRAKTCMTEEQVLRLAGL
                     AIDIERYFKRPQDIEWALGKDGKLYILQSRPLRITEADIRPKKDLSLSELSKKYPVLI
                     KDKGYVVQKGIAVGRVFILKNAEEIDGLPKGVILVARNDSPQFVRAMPYVSAIITDTG
                     APASHMASICREFRIPTIVNTREATHLLQHGQEITLYADEEGSFAVYDGLLDEMIAFR
                     EEARQALEELYEFRRKKYIMRFISPLNLVNPLTDEFTPKKCKTMHDVLRFMHEKSVQE
                     LIEASMNIAGKGGLKKLELPIPAGIHVIDIGGGLHMESGEKATLDQVVSEPLRAVVQG
                     MLHPGAWHSEAVALQARDFLTSMMRMEEITSEGIKYASRNVAVISGEYLNLTLRFGYH
                     FNLLDCFCSEKAANNHIYFRFVGGATDISKRSRRIRLIAEILKEYGFNSSTKGDLIIA
                     RLSHIEKDEILNILDMAGRLIAFTRQLDAVLNDDSDIERYKKNFFKENFTLG"
     gene            13414..14985
                     /locus_tag="VMU21_06745"
     CDS             13414..14985
                     /locus_tag="VMU21_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77259.1"
                     /translation="MSLKKKITLSFFISAFIIAALSTFEYINFIEIRKEIKFLELTDT
                     IRSKSLQLRRHEKNFFLYGPLTSGTEAEAIHTYITELDSILAGNMAMDKTGKLALLKN
                     SIAEYGQRFRRIETSIQELSKELRDKSFFRKRYSALFSLMEVAMFDRPLQGAEFLEKE
                     FLLSPQDRIIGQLKVLDSDINALRKNGEEILAISKEIDRIARESAERVTRISKIAILI
                     FFPLFLVTGIGMLFFISRSVASRLNQLISLVEKTGKGQFSTMLVPFERDEVGLLIRKF
                     NEMEEQLAHREAEIEKKNNELLQSKKLAAIGTLASGVAHELNNPLNNIYLSSQMLEKE
                     MGDSTSPAVREIVGDIAGQTRRMKQITGDLLEFARGREPHLREVELGELIMGAYKLVS
                     AAMDTRKVHLITDTDAGGILMDADPEQMERVFINLFTNAVDAMSGEGTIRVQALKGQR
                     EVRITVSDSGRGMPPEIVEKIFEPFYTTRDKGTGLGLAIIFSIINKHHGEISVESKPG
                     EGTTFTITLPIRKGQ"
     gene            14985..16316
                     /locus_tag="VMU21_06750"
     CDS             14985..16316
                     /locus_tag="VMU21_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO77260.1"
                     /translation="MNFRVLIAEDEEITLKHLVNALKREGYDVMGTHNGREALRELSK
                     NLYHVLITDIKMPEMTGIELLEKAKEMNSEIEVLVITGFGSIGSAVEAMKKGAYEYIT
                     KPFDLDDLILKVKNIRERSTLKKENVALKAYFGMDKKVSIIAKSESMKQILAMIEGIK
                     GSDCNVLLTGETGVGKSLLAKIIHFTSRRQNMPFLSLNCATLTEELLASELFGHEKGA
                     FTGAIRTKQGLVEIADSGTLFLDEISEMAPNLQAKLLKVIEEGEFYRVGGTRPIKVDV
                     RFIAATNQNVKSAIAEGRLREDLYYRLNVMEISISPLRDRKEDIEPLSEHFLTKHLPE
                     SNKNISGFAKESMDVLKSYSFPGNVRELENIIERAIIIEKDSLITPESLPQTLKIFQI
                     ETFEPERIKTIDELNKDYAERVLELAGGNKSKTAELLGISRTSLWRILKEE"
     gene            16356..17684
                     /locus_tag="VMU21_06755"
     CDS             16356..17684
                     /locus_tag="VMU21_06755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO77261.1"
                     /translation="MGLKIIIAEDEDITRKHLLHALKKEGYLAVGAKNGREALELIHQ
                     EHFDVLITDVRMPEMSGIELLEAVKKGYSAMEVMIVTGFGSIDAAVEAMKKGAYEYIT
                     KPFNLDELILKVKNIHERKILKNQNLFLRTFLGMHKGVSVIARSESMQRIVNSVEGMK
                     DSDCNVFVMGETGVGKSLLANIIHSTSTRQNMPFLSLNCAALGEEPLTSELFGHEGGA
                     FPGVGTTRQGLIEISDSGTLFIDDVTEMSPRNQTKLLKVIEDGELFRIGGKIPIRINV
                     RFIAATHRNIRECIAQGTFREDLYYRLNIMEIVIPPLRERKEDIEPLCTYFLQKHLSE
                     STKRISGFTQEAMDILMEYSFPGNVRELENIIERAIILEQGTHITPQSLPVSIKKFRI
                     DTFSPEGVKTIDELTKDYTTRVLEMVDGDKDKAAELLGISEITLWRILKG"
     gene            complement(17700..>17962)
                     /locus_tag="VMU21_06760"
     CDS             complement(17700..>17962)
                     /locus_tag="VMU21_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77262.1"
                     /translation="LLKMYQDGFLLWKYHTEFSSHNFVPKGRIYVGQDVEPIVVKYRL
                     PTETHIFEPTQQTWKSIAEDSIQIIWDNADAQVKRINTLLNE"
BASE COUNT         4934 a         4059 c         4731 g         4238 t
ORIGIN      
        1 attaggttct accgttagat attttttcct tgggtcgtat gtatcacccc tatttggagc
       61 ctcaagaacg aatagaatat caattgtcct cggaggcgcc ttcatcagga ccggaaatgc
      121 ctctgggtcc aaaaggcctt catctttaca gcggccgcat tgaccgcact ctgccgcaaa
      181 ttctcgccac atggttcatc ttcctcgggc acaaccagtt attagtagtt tcctgctaat
      241 actccttttg taatgttatc cattctgtta atgcgactaa tatccgaaat ctcatgttag
      301 tcaattagat atacttcgac cttcccgtaa ttaaatctga taataactta caccttttta
      361 aagtttttga aaagcgctat aaactacaac tcaaaaatct tgaacatggc agaaaggatt
      421 ctggtcactt cccattatgc ttcaaagtaa ctaagactca aagcctgtgc atggcagttt
      481 caccactgga ggaggtggcg agcacatagg cccttcctct ggggggaacg ccagtacgtg
      541 ctctcaatac tatttcagtg ccgcctataa gctaaaatgt ttcctaatga agtatactca
      601 ggaaatcaag gcaataataa ttccagcctt gcacgttttt caggttgcgc cttagtagtt
      661 tgcggagcgt cagatgggac acgatttatg gttaagcctc ttcggttttt tagtgggtat
      721 gtttggtacc ctgatcggtg cgggcggggg atttattctc atgccggtcc tgctgctgct
      781 ctatccccat gacagcccag aaactataac cagcatatct ctgtcagtca catttgcaaa
      841 tgccttttcc ggaacgatgg catacgcgcg tatgaagcgt atccactata cgtacgggct
      901 tattttcgct gctgctgcaa ttcccggtgc catatcaggt gcattagtca cctactatgt
      961 ccacagaaga acctttgatt tcatctttgc catcctgttg ataatcgctt cagtcatact
     1021 atttctcaag acgccaatgg gacgaggctc agatgcgcac agatcggttc agctgtctcc
     1081 ccgctatttt cgcctaggca ttctaattag cgccgctgtc ggttttgtat ccagcttcct
     1141 gggcatagga ggagggataa ttcatgttcc tgcgcttgca ggcattcttg ggttccccgt
     1201 ccacatcgca acggcaacat cgcatttcat attggcaata gtgacctttg caggagttat
     1261 ggtccatatc ataactggca ctttccaaca cggtattgta cgggctattt ttcttgcgtt
     1321 cggggtcctt ttcggtgcac aagccggcgc tttattctct tcgaaagtgg aagcgagaat
     1381 aatcattcgc ttattagcgg ttgctctggc actggtagga atccgcttac ttcttctttc
     1441 gctcaatgag ttctgagata ataggatatg gttggtaggt atggcagcct cctgttaatg
     1501 atctccggtt ggtatccttg tattaatgtt cgagatattc aggcctccga ccgatgcagg
     1561 agattttggt gttcataatt gaaaccctat cctaagtcgt tttgcgttgc tgagatgtcc
     1621 caatagcaaa aactcttgaa ctggaattcc tcgcttctaa aggattaacg gctccttcca
     1681 taagtcttag ccccatagtt gcattctttt gagtgttcag cactgttcaa atatgaaaca
     1741 tttaacatgc tgttccagcc taatttatcc ttataaatca acaatataca ttctggcacc
     1801 ctccttgcaa tttatgcaga tacgaaagga ggctgagatg aaaggttata gaaagatagt
     1861 aatcgcagta aacggctcta aagatgttct cactcaagga ctaaggcttg cccaggacga
     1921 gaagtgctgg gtaactgtag taaaggttgt gccgccccat aatggagacc tcaatctggt
     1981 cggcatcagg aatatcggcg acgtactgga taacggggcg aacaatgaga ggaatgaaat
     2041 aatgaatatc gctgaggcag aaagcgctct ggtcaagacg cgcgtcgaag agggcgatat
     2101 ccctgaaaag attgtggagg ttgcagaaga tgagaagtgc gacctgataa tcatgggaac
     2161 gaataaaaca agctggttca aaaaactgtt tggtaagaat gtggtggaaa gggtcatcag
     2221 tcaggccccc tgtcccgtct tagtggtcgg agcataaagg aggaaaccat gagggaaaag
     2281 cagcttttgt ttgtcaccta ccatgacgag aacttagatg aagggctttc atatgctctc
     2341 gaccttgcga aaacaatgaa tgacggtatc gagatattag tggtttacaa gagaaaggtg
     2401 ctggaaaaat tcgaggacat gatgaccgca atcacctttg ccgagtctga catgcacaag
     2461 accgcgaagg agatgataag tgccgactac gaaaagaata acgaagatta tgaaaaaagg
     2521 atagcccttt tagaggaaca atgcaggaaa gccgggatcc gggtctcaat cagtacggca
     2581 gcaactgacg cagtttctgc cgtaaaaaac atcctcaggc agaacgtcag gatcgatatg
     2641 gtacttttaa gccccagcat aacaaatggc ggaaacgtga cctcaaaggt tatcaacaag
     2701 ctcgtaaaaa ccgcatcaag gccgatagtc accatggcaa aaaatataca cgcagcatga
     2761 aagaaaaagg agggagataa atgcatcttt atttacctgt agcgctcaca agcattaatg
     2821 tcttaatccc tgtcggcctt ggcctggcgg tggggctgct ttctggtctt tttggcgtgg
     2881 ggggaggatt tctcatgaca cccctcctga taatgttcgg tattccttca accgttgcgg
     2941 ctgcaaccga ctctaaccag atcgttgccg catcaacttc agggacttat gcccactgga
     3001 gggtcggaaa cgttgatttc aagatggggt tttatctcct cgtcgggggt tttaccggag
     3061 gccttttcgg ggtgcaggcc ataaaggttc tcaatgcaat ggggaatgct gactttgtga
     3121 taaaaatgac ctacgtgttc atgctcggaa tcgtgggtat atacatgttc atcgaaagtc
     3181 ttgcgagcat gaagaagaag cagccggaga cggtcgcacc agcaaaagaa tcacgcatga
     3241 cgagatttct taagtccctt cctttccaga cccattttga aaagtcaggc gtaagccatt
     3301 ccacgctttt acccatcttc ttcggcgggc tcgttggaat tcttgccgca atcatggggg
     3361 tcgggggcgg ttttcttatg gtgccggtaa tggtttacat cctcagaatg cccatgcatg
     3421 tggtagtggg cacaagcctc ttccagatac tctttaactg cattgaggtc acgtttcttc
     3481 aggcttacac gaaccatact gttgatttta tccttgcggt tctcctgctc ctcggttcca
     3541 ccgtgggcgc ccaggtgggt gcagtatttg ggaggaagct taagggtgag cagctcaaga
     3601 taatccttgc ggtgatcgtg cttgtggtga cggtaaagat tgtctttgat ctcacgctta
     3661 gtccatcact gcttttagct cagggaggag gacactaata tgaaagttac cattttgaaa
     3721 tccgggctga aaaatacgat cttcttgggg gttgctctca tggttctcgt gctcggtgct
     3781 gcgggaaacg catcagcgga actgactgca acggcaaacc atgaccatat taatgtagac
     3841 tttttttatc acggcagctc ggtcagtgtg agagggaccg cagaccaggg aatcgacctg
     3901 gttataaaaa tcacctcgcc tgaaggtcac caggaactgc tgaaaaaggg gaagttggca
     3961 gggttcttgt ggatgaatgt gggaaaattg actttcgaga agacgccgaa gttctacgga
     4021 attcacagta caaaaaggct tgaagatatt ctgagccgcg atgaaatgga taagtatgct
     4081 ctgggatatc ctgcgcttga aaggcatgtg gagataagtc ctgtgggcag cgagggcgaa
     4141 aagtcagact ggtttaatca atttgtaaaa ttcaaagaat cttcaaagct ctattcagtg
     4201 tcatccggaa aaattaatat gaccgcacaa ggggggaagc aaaattacta tgtgcttctg
     4261 gactggccct atcaggcact tcccgatcat tatacgataa gcgtctatgc ggtcaaggat
     4321 aagaaggtga ttgaaaccgc agagacacat gtccttgtgg agcaggtagg aacagtgaag
     4381 tcattggcag ggatggccaa gaacaacggc tcattatatg gaataatctc catacttgcc
     4441 gcgctcggtg cgggatttgg tgtcggtatg gtattcagaa aggggggcgg ggcgcattaa
     4501 tacacaagga ccactccttg agtatcccct ggagaagata tgggaatgtt gatacacaca
     4561 gatagcgcgg tgaagacaat gtatgaaact atcctcgttg gctttgatga atccgattcc
     4621 agcaaggcag cactgagtga ggcagcacac tggataaaac ggcatggagg gaaaatcatt
     4681 ctcgtccatg cggtgtactt tgatgaagag gagttcggga ttgcgccgga ccaactggac
     4741 aggcgtctca aggtggggga aaaaatatgc gttcaggcga aggaaagcct tgcaaccggg
     4801 ttgggcatag aggtgaactc attgctctgc gaaggtgatc cgcccgaggt catccaggat
     4861 gttgcaaagg ggaaaaaggc cgacctgatt gttctgggta cctatgggag gaaggggtta
     4921 aagagacttc tcatgggaag tgtgacatca caggtcattg tcaattcccc tactgatgtc
     4981 ctggtggtga aaaagccatg cactgaatgc tcgggaacat acagatccat tttggtcccc
     5041 tttgacggct cggtatctag tgaaagggca ttggcaagag catgtcatct ttcaaagatc
     5101 gacaatggag agattactgc actctatgta atccccaggt acgaagagat ggtggaattt
     5161 ttcagaacca cttcaataca gaaaagcctt caccttgagg cccaaaagat tattcacaag
     5221 gcaaaagagc tggcctcagg agagaatgtg tccataaaga cgcttatcga tgaggggaac
     5281 gcaggagaga agatcatcga aacggcaggc cttaacagta gcgatcttgt ggttatggga
     5341 gccagcggct ggaggggtgt gaatagagca atcatgggaa gttccacaga acgggtaata
     5401 atccacgcca cctgccctgt ccttgtggtg aggtgatacg gataaacatc ttcagactaa
     5461 ttctaaaaaa caggccctct agcgctccta ttggcggcgc acagcgcatg cctttggtat
     5521 aggcataggg aatctgttat ccggaggtga aagaaatgct tgaaggactt tttcagccaa
     5581 tccaatgcat ctcatagtga ttccggcaat cggcttggta gtgttcggac caggaaagct
     5641 tccccagata ggtgcagatc tcgggaaaag catacgggaa ttcaagaagg ctatatcgga
     5701 taaggctgaa gatttaaatc catcctaacc caacacaatg ccagaaaccc cggccaaaga
     5761 agaatagcaa aagaaagggg tgtcggctct ggcctcagac acgctggaac aatgctaaag
     5821 aagccctcca tactatttcg gcgcggttat gaactgtcaa gaaactccaa tgaggagatt
     5881 cgaaaggctc gtcaagaggg aacttcttac tcaagagaag caagaaagac ttaatggtat
     5941 aatcaggaag ttgcgagggc cgtgagaaca atggttgaat ataaattcat gaggcttgtg
     6001 agacgtgggt tgctgaccaa ggaagaactg gacaacgcta tcctcgaatc ggattcttcg
     6061 aaaaaataca tagaagatat tctcctgaag aaaggaattc ccagacacga gatcctcctt
     6121 tgcatatccg agttctataa ctgccccttt gtcgagtatg acgaagacat caccgtgtcc
     6181 cgaggcgtct tgcggcttgt cgatccctta agtctcaaga acgagctctg gatcccgctc
     6241 tctgtcttta acagaaaggc cggggtgatc gcatatagac cagaagaccg tgcgctggcc
     6301 gaaaaaatca gagagatgct cggcgcagac gaaatcgaat tccgggccgg gctaccgaat
     6361 gatattatca ggatcataga gaacaacttc gatctgaacc ccgatttccc gcctgcagcg
     6421 ggcaggaccc cgcttgccaa ggtcaggact tacctggccg aaaggcgctc actgctcgca
     6481 gagcaaagga catcccttgc gaggggaaga accggacttg cgttccttcg gaccggctta
     6541 gccttcatag ccatagcagt gacctttttc agggtattcg gcgcgggata tctctccata
     6601 atcgagcttt tgctcttctt gggcggggtg gccgcggtct tcgatgggct taaatggtac
     6661 atgcccgtcc gcaagaaagc cggaaaggta ttggattaca tatctcaaca gtcccctccc
     6721 ggattcacgt gcctggaggt atcaaatccg ggggaggatc ccgtcttcac cagaagcgag
     6781 gtcgtagaga gcgcggagga cctccgggct ggatggggga aattgtctcc cgtggagcgc
     6841 aggagattcc ttgccaatga cagaacggat ttggccgaag aaaggaccat acttgccagt
     6901 gtgcgcacac gtatggccaa ggctcgtaca ggactggcct ttgggcgaac cggcatcgca
     6961 ttttctggcc ttgggattgc cctgttaagg cagttccgcg aaagcagctg gacttttttc
     7021 gatatcgctt tgatattggc aggggcactg atgacccttg aaggtgtaat atggtacttt
     7081 tccgggagaa gggcagggaa agcaggatta aaggccgttt taaaggctga agagcaaatg
     7141 agcatctggg agtccgcttt ccctccgttc tacaggtcga gccccgtctg ctttccccct
     7201 gtcaaggcct gtcatgctcc gggagtatgg gccaccacag gacttgcgct cgaaaggact
     7261 gtcctcgccg aaaggagaaa cataatggcg aggttaagga ccattatggc ccgttccagg
     7321 accggaatgg cattcatcag gacgggggtc agcctttcgg ctatcggtgc aggactgctc
     7381 atatatttcg gcgcgtccaa tctcgcgtgg acggcctttg acatggcgct tatatttgcc
     7441 ggcattgtgc tcatagccga tggagttcat tggtatctac ccgccgaaaa gaccaaaggc
     7501 caattccctt attgttacgg agatttggag atcaggatac ccgattacag tgtgcctggg
     7561 cgaagctgga agaaggcggt gttcagccat gatgacatct aagagcgggt tgaggtttgg
     7621 ccacctggtt gaggaagggc tcctgacgga aaaggagatc aacctggctt atgacgcctc
     7681 gatggcgcgt ggtgtgaacc tggagaggat acttctacaa gaattcggcg tgccccggct
     7741 gtctctcctc gaagcccttt cccgacatta cgattgtcct tacattgaat acgacgagag
     7801 gcttccggtc ccgacagagg ttttcgaagg actgaattcc gatgttcttt cgcggagtct
     7861 ctggtttcct cttataaagg aagaaggcgc ggttaccata gcggcgaatg acccccggag
     7921 cccccgggtc agcgaagagg cccgcgcgca ttttccagag gttcggcttg agtttagggt
     7981 tgccctcgca agcgacatac aatggtatat acgggatttt cttcacgcaa agcccaagtt
     8041 tctcatcggt atagaaagga cagggctcgc cttctggcgc aatacgatgg ctcattggag
     8101 gacgaggctt gcctgctaca ggactgacct cgcaaagggg aggacatctc ttgctatctt
     8161 aaggtgggga ctgggcctta tagccttttc cgacgtgctc ttaaggactc gcaaagacct
     8221 ttttatgatc acgtatgcat attgggccat gatgcttgct ggtcttacgt tctcgatttt
     8281 cggattcgct aagtatctgc aggtgcgtag gtcaagaata agccctccga agcaccatac
     8341 cctcgtggaa gtgacagcgg ccactacgat gttccttgag aattatcatc ttgaaggggc
     8401 ttcggctcaa aagaggacaa agggcactat gcttgcgcgg cttagcgatt gcctgccgaa
     8461 ttattgcaca atactgaacc ctgtcccggc gagcaaggag cggacccacc ttgcccgcga
     8521 aagaaacgtg cttgccgcgc aaaggacgat cgccgcctgc tatcgcacta tgtattcgcg
     8581 ggcaaggaca gggttggcct tcatccgcac aggcgtcgcg ttcttcagcc ttggacttgg
     8641 ttttctggag tattttctct tcggtctttt cagtatcata gattcttctc ttatgatcgc
     8701 ggggctcctg atggtcgccg atggcgtcct ctggtatatg ccggcaagga aagaacagtc
     8761 agaactcccc agatgccctg tacctcaata aatgatagga taaaaacatg aagatagaac
     8821 acaggatcat cctctccaat gtcttcaatg tcgcacttat agtgctcatc ggtctctttg
     8881 caatacagaa cctcaatctc atgctcacca agctgaggtt tgtggagatt gccgacgacc
     8941 tgaatgcgtc atttctcgag atgaggctgt ccgagaagaa ttacttcctt tacaaagacg
     9001 aatcggcact ttccgggata aaggaaaaaa ttgagaagac catggagtcg gtagaagcag
     9061 tgggtgatga tatatcccgg gcggtgggcg aaaataatct caatcagctt ctcgcatacc
     9121 ttaagaacta ttcagggatc gtggaagagc tcaggagagg cagcagggac gtgcaactcg
     9181 agacaaggct gaggaatgca ggaagaaaac ttaaggaatt ttccgagacc atgactgtcc
     9241 tggagaagaa gcaggtcaat gatattattg cccggtctaa gaaggttctc ttctcttcat
     9301 tctgggcgat acttatctca gcaatcgcgg taagccattt catttctcaa aggatattac
     9361 gatccctgag ggaaattcaa cgtttggcga tcagcatatc tgaagggaac ttcagaacaa
     9421 tagagcatat cccctcgaga gatgaattcg gttcattaat gaaggcgatc aattttatgt
     9481 ccgaagagtt gaagaaccgg gaggaggaga ttattcagtc aaaaaaactc gcctctctgg
     9541 gggtactcac tgccggggtt gcccacgaat taacaaatcc cctcaacaat atctccatga
     9601 ttgcacagac ctatgaggag gtgtacgacg ggctgagcaa tgagcagagg atcgaattca
     9661 tgaacaaggt cgagagggaa accgagagga taaaagagat cgtgaagaac ctcctggact
     9721 tttcgaaacc aaaggaagtg gttctgaagg aggctgatat caataccgca attcagaaga
     9781 ctttgaagct agtgcaaaat atgcttgaca tttctaatgt ggaaacgaaa atagttcttg
     9841 aagacagcct cccccctgtc ttcactgatg aacaccagtt tcagcaggtg cttgtaaatc
     9901 tcattaccaa tgcagttcat gcaatgccgg gaggaggaag gcttttcctg gggacaaggg
     9961 caagaagaca aagggacact attgaggttt cggttatgga taccggaaaa ggcataccgc
    10021 cagagcatct cccccatata tttgatccgt ttttcagtac caagggcgaa ggcggaacag
    10081 ggcttggact gtcggtcagc tacggcatca taaagcatca tgggggtact atcagggttg
    10141 aaagcaaggt gggtgtaggc actaccttta ccatagagtt gcccgcctac aaaaaaaagg
    10201 aggagaacaa tggatcgtta tagaatcatg gtcatcgatg atgagaagat cgtgggtgat
    10261 atggcaaaga tatcgctcga gcaggacggg tatgaagtgg aaacctttct caatcccgcg
    10321 cctgccttgt caagattgaa agagatgaaa ttcgacgttg tggtgaccga ttacaaaatg
    10381 aagggcatag acggcatgga agtactcaag accgtcaagg ctctatatcc agagaccaag
    10441 gttatcatga ttaccgcatt tgcgaatctt gatactgcca ttgaggcgtt gcggggtgac
    10501 gtgcacgact ttttcccaaa acccgtgaag ataagggaac tgaaggaatc gatcaggaag
    10561 gccctgtcca aataatcata tcaaaatctc atgaaaaacc tgtctgtcat actaaaacag
    10621 gccttcggga aaaggagccg cggcagtccg gtggatatgc ggacttttct tcacaatttc
    10681 aagaccgccc tcgaaagcaa caaccgtgcg ctcgaaataa tcaccgacat gggagaaaaa
    10741 ctgagcggtg aatacctctt cgatataaat tacataagaa aggcctacgc agagcttcat
    10801 gcgaacatac gctcttccat aactacgttt aattccctta cgcggaacag atacgagatc
    10861 ggccctgcgt gtgaccggat agattctatg ataataaggg tgatatacga agaggccccc
    10921 tttcaggccg gccgggtcgt cttttatgaa gggataacat gggagatggc gcgtgaggtg
    10981 ggagggaaga attatcatct ctcgcagcta agaaaagacc ttgacctgaa cgtaccgggg
    11041 gggttcacga tcactgctct tacctatgat gaattcatca gacataacgg catagacaag
    11101 aaaatagagg aactgagttc aagaccatat ggcgataaag acctccaaga catcagggac
    11161 gccataatcg ccggagagtt tcccgaaccc gccggttccg agatagaaaa ggctacggac
    11221 aaacttaaag acccgggcag gagcaacgag tttctggccg tcaggagcag cgcagaggag
    11281 gaagacgggg atttctcctt tgccggccag tttgagacca ttcttaatgt cccgctggaa
    11341 agctttgcgg tcaaggccgc ctacaaaaaa gttatatcaa gcctttttgc acctaccgcg
    11401 gtttcttatc aaaaacggct cgggtatgca atcggcggca tgcggatgcc ggtcggttgt
    11461 gttttgatgg tagatgcagt ggcaagcggt gttgtctaca cgtcggatcc ctcaggcggc
    11521 agaaacaggc tgaccataat cgcggcgtgg ggattgggga agtctgttgt ggaaggcaag
    11581 gtcgaacccg atgtgtactc gctcgaaaag gggcctgctc tcaaattgat cgggacaagg
    11641 gtcgggacaa aggaaaccat ggtcataaat tcctcaagcg gcgggactgt tgagatgaag
    11701 acgccggcag acttacgggc gaagacctgc atgacggaag agcaggtctt gaggcttgcc
    11761 gggctggcga ttgatatcga gaggtacttc aagaggccgc aggatataga atgggcgctc
    11821 ggcaaggatg gaaagctcta catccttcag tcaagaccgc tgagaataac tgaggcggac
    11881 attcgaccaa agaaggactt gtcactatcg gaactgtcca aaaaatatcc cgtgttgatc
    11941 aaagacaagg ggtatgtggt gcagaaaggc atagccgtag gcagggtctt catccttaag
    12001 aatgccgaag agattgacgg tttgcccaag ggagtaatcc ttgtcgccag gaacgactct
    12061 ccccagttcg tacgagccat gccgtatgta tcggcgataa taaccgacac tggcgcccct
    12121 gcgagccata tggcatccat ttgcagggaa ttcaggattc cgaccatcgt caacacgaga
    12181 gaagccactc atttattaca acacggccag gagataaccc tctatgctga tgaggaaggc
    12241 agtttcgccg tttacgacgg ccttctcgat gagatgatcg cattcaggga ggaggcgcgt
    12301 caggcgctcg aagagcttta cgaattccga aggaaaaaat atatcatgcg ttttatttcg
    12361 cctttgaacc ttgtaaatcc gctcacagac gaattcacgc ccaagaaatg taagaccatg
    12421 cacgacgtcc tgaggttcat gcacgaaaaa tcggtgcaag agttgattga ggcttcaatg
    12481 aacatagcgg ggaaaggcgg gttgaagaaa ctggaacttc cgatccctgc agggatacat
    12541 gtgatcgaca taggcggcgg cctgcatatg gagagcggag agaaggccac gttagatcaa
    12601 gtcgtgtccg agcctcttag ggctgtcgtg cagggaatgc ttcacccagg ggcatggcat
    12661 tcagaggcgg ttgcactaca ggcaagggat ttcctgacga gcatgatgag aatggaagag
    12721 ataacgtctg agggaatcaa atatgcgagc aggaatgttg cagtgatctc gggagaatat
    12781 cttaatctca ccctgaggtt tggataccat ttcaacctgc tcgattgctt ttgcagtgaa
    12841 aaggccgcca acaaccatat ttatttccgt ttcgtgggcg gcgcaacgga catatccaag
    12901 cgctcgagga ggatacggct tatcgctgag atattgaagg aatatggctt caactcgtcc
    12961 acaaagggag acctcataat agccaggttg tcccatatcg aaaaggacga gatactgaat
    13021 atcctcgaca tggcgggaag gctgatcgcc tttacaaggc aactcgatgc cgtgctgaat
    13081 gacgacagcg atatagaaag atacaaaaag aatttcttta aggaaaactt cacgctgggt
    13141 tagccctact cagggggttc agccaggtcc acatggcttt tccaaaaggg actgtcttgt
    13201 cgagggaggg ggccatctga gaggacgtct attccttgcg attcgtgaga ccggtccact
    13261 tcatgtagcg ggcaattgga tgcggtaatg catgatgact ttgccgtcga acggcatgtg
    13321 agaaacttcc tcaaagagaa ctatgaattg tgagaatgcg gttatacttt ctcctctcaa
    13381 tggtataaaa taccttgaga gagatcctaa tcaatgtcac tcaagaaaaa gataaccctc
    13441 agctttttta tcagcgcctt tatcatcgca gccctctcaa cctttgaata tatcaatttc
    13501 atcgagatca ggaaagagat caagtttctg gagctcaccg acacgataag gagcaaatcc
    13561 ctccagttaa ggaggcacga gaagaatttc ttcctctacg gacccctgac atcaggcaca
    13621 gaagcggaag ccatccatac ctatattact gagcttgatt cgatcctggc cggaaatatg
    13681 gccatggaca agacagggaa actcgccctc ctgaagaact ccatcgcgga atacggacag
    13741 agattcagaa ggattgagac atccattcag gaactctcaa aagaacttag ggacaaaagc
    13801 ttttttcgta agagatattc agcactgttc tctctcatgg aggtggccat gttcgaccgg
    13861 cctcttcagg gtgcagagtt cctggaaaaa gagttcctgt tgtcacccca agacagaatc
    13921 atcggacagc tgaaggtatt ggattcggat atcaatgccc tcaggaaaaa tggtgaagag
    13981 atccttgcta tctcgaagga aattgacagg attgcaaggg agagcgctga gagagttact
    14041 cgcatatcaa aaatcgccat cctcatcttc ttccctctat tcctcgttac cggaatcggg
    14101 atgctctttt tcatcagcag gagtgttgcc agccgcctca accagctcat cagccttgtg
    14161 gaaaagacag gcaaaggaca attctccaca atgctcgtcc cctttgagag ggatgaggtg
    14221 ggtctgctta ttcgtaaatt caacgagatg gaggagcagc tggcccatag ggaggcggaa
    14281 atagaaaaaa agaacaacga actcctccag tccaagaaac tggcagccat cggaaccctt
    14341 gcgtcaggcg ttgcccacga actcaataac ccccttaaca acatctacct ctcgtcccag
    14401 atgttggaaa aagagatggg agacagcacc tccccggcag tgagggagat cgtgggtgat
    14461 atcgccggac agaccagaag aatgaaacag ataaccggcg accttctgga attcgccagg
    14521 ggaagggaac cacacctgag ggaagtggag ttgggtgaac ttatcatggg ggcgtacaag
    14581 ctcgtgagtg ccgccatgga tacccgaaaa gtgcatctta tcaccgacac ggatgcgggg
    14641 ggcattctca tggacgctga ccctgaacag atggagaggg ttttcatcaa cctcttcacc
    14701 aatgccgttg atgccatgtc cggagaaggt accataagag tacaggcgct caagggacaa
    14761 agggaagtga gaattactgt gtccgattca ggaaggggaa tgccccccga aatagtagaa
    14821 aagatattcg agccattcta tactacgagg gataagggta caggtctcgg actcgcaatt
    14881 attttcagta tcatcaacaa gcatcatggt gagatttccg tggagagcaa gcctggggaa
    14941 gggaccacat tcaccattac attaccgata aggaagggac aatagtgaat ttcagggtac
    15001 tcattgcaga ggatgaagaa ataaccctca agcacctcgt caatgcactg aaacgtgaag
    15061 gatatgacgt aatgggcaca cacaacggcc gcgaggcgct ccgggaacta tccaagaatc
    15121 tctatcatgt tcttatcacc gatataaaaa tgcctgaaat gaccggcatc gaactcctgg
    15181 aaaaggcgaa agagatgaac tccgagatcg aggtgctggt cattaccggg tttggaagta
    15241 tagggtcagc ggtggaggcc atgaaaaaag gggcgtatga gtatatcaca aagccctttg
    15301 acctggatga tcttatcctt aaggtaaaga atattcgtga gcggagcacc ctgaagaagg
    15361 agaatgttgc tttgaaggca tacttcggga tggataaaaa ggtctccata attgcgaaga
    15421 gcgaaagtat gaagcagata ctggccatga tcgaaggcat aaagggttct gactgtaatg
    15481 tgctcctcac cggggagacg ggggtgggga aaagccttct cgcgaagatt atccacttca
    15541 ccagcagaag gcagaacatg ccctttcttt ccctgaattg tgcgacgctc accgaagagc
    15601 tccttgccag cgagttgttc ggacatgaaa aaggcgcatt cacgggcgcc atcaggacga
    15661 aacaggggct tgtagagatt gcggattcag gtacactctt ccttgatgag atttcggaga
    15721 tggcgccgaa tctccaggca aagctcctga aggttatcga ggagggggag ttttaccggg
    15781 tcgggggcac acggcccatc aaggttgatg tcaggttcat cgctgcaacg aaccagaatg
    15841 taaagagtgc aatcgcagaa ggaagattga gggaagacct ctattaccgt ctgaacgtta
    15901 tggagatatc tatttcgccc ctgagggacc gcaaggagga catcgaaccc ctgagcgagc
    15961 atttcctgac gaaacacctc ccggagtcga ataaaaatat ctcagggttc gccaaggagt
    16021 ccatggatgt tcttaaatcg tatagtttcc ccggaaatgt gagggaactg gaaaatatca
    16081 ttgaacgggc catcatcata gaaaaggatt ctcttatcac tcccgaaagc ctgccccaga
    16141 ccttaaagat cttccagatc gaaacctttg aacccgagag gatcaagaca atcgatgaac
    16201 tgaataaaga ctatgcagaa agggtcctcg aactggcggg tggcaataaa tcaaagactg
    16261 cggaactcct gggcatatcc aggacaagtc tctggagaat actcaaggag gaataatctt
    16321 gccatgcaca atccccttta ccacagagcg cccgcatggg tctgaaaatc attattgcag
    16381 aagacgaaga cataaccagg aaacaccttc ttcatgcgct gaaaaaggaa ggttacctgg
    16441 cagtgggggc aaagaacggc cgcgaggccc tggaactgat tcatcaggag catttcgatg
    16501 tccttatcac ggatgtcagg atgcctgaaa tgagcggcat tgaactcctg gaagcggtga
    16561 aaaagggcta ttcagcaatg gaggtaatga tcgtcacagg gttcggaagc atcgatgcag
    16621 cggtggaagc catgaaaaaa ggggcctatg aatacatcac gaagcccttt aacctggatg
    16681 aactcatctt gaaggtaaag aacattcatg aacggaagat cctgaaaaac caaaatcttt
    16741 tcttgagaac cttcttaggc atgcacaaag gggtctcggt cattgcccgg agcgaaagca
    16801 tgcagaggat agtgaatagc gtggagggaa tgaaggattc ggactgtaat gtcttcgtta
    16861 tgggggagac cggggtgggg aagagtcttc tcgcaaacat tatccactcc acgagcacga
    16921 ggcagaacat gccctttctt tcgttgaatt gtgccgcgct cggcgaggaa ccactcacca
    16981 gtgagttgtt cggacacgag gggggagctt tccctggcgt cgggaccaca agacagggac
    17041 tcattgaaat ttcagattca ggcacactgt tcatagacga tgtcaccgag atgtctcccc
    17101 gcaaccagac aaaattgctc aaggttattg aggatggtga acttttcaga attggaggaa
    17161 agataccgat caggataaat gtgcggttta tcgctgccac acaccggaac ataagggagt
    17221 gcatcgcaca gggaacattc agagaggatt tgtactaccg gttgaacatt atggagatcg
    17281 ttattccgcc tctgagggag cgcaaggaag acatagaacc cctttgcacc tacttcctcc
    17341 agaaacatct ttcagaatcg acgaaaagga tatcagggtt cacccaggag gccatggata
    17401 tcttaatgga gtacagtttt ccgggcaatg tgagggaact ggaaaatatt atcgagaggg
    17461 ccattatcct tgaacaaggg acccatatca ctccgcagag tctgcccgtg agtatcaaga
    17521 aattccggat cgataccttt tcgcccgaag gcgtcaagac cattgatgag cttacgaaag
    17581 actataccac gagggtcctg gagatggtgg acggcgacaa agacaaggcc gcagaactct
    17641 tgggtatctc ggagatcacc ctctggagga tattgaaagg gtagatgggt ctgaacgggt
    17701 cattcattta atagggtatt tattctcttt acctgcgcat cggcgttatc ccagattatc
    17761 tggattgagt cctctgctat gcttttccat gtctgttgtg taggctcaaa tatatgcgtt
    17821 tcagtgggaa gccggtattt caccacaatg ggctccacgt cctgccccac atagatacga
    17881 cccttcggga caaaattatg actgctgaat tctgtatgat atttccacag gagaaagcca
    17941 tcttggtaca tcttcaggag tt
//
LOCUS       DATKOU010000149         9356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2894018, whole genome shotgun sequence.
ACCESSION   DATKOU010000149 DATKOU010000000
VERSION     DATKOU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2894018"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VMU21_06765"
     CDS             <1..257
                     /locus_tag="VMU21_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77263.1"
                     /translation="HESFTELLLAVFHLVEELVVREDVTSLPDADRHHIAGDIKRAYI
                     LLVQQWLEYMGHLRDHYPYLFSLAIRINPFDENASPIVKS"
     gene            556..3120
                     /gene="uvrA"
                     /locus_tag="VMU21_06770"
     CDS             556..3120
                     /gene="uvrA"
                     /locus_tag="VMU21_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUO77264.1"
                     /translation="MQDHIIIKGAREHNLKNIDLSIPREKITVITGPSGSGKSSLAID
                     TIYAEGQRRYVESLSAYARQFLEQLQKPDVDSIEGLSPSIAINQKTVSRSPRSTVGTI
                     TEIYDYMRVLYTRIGRPFCFLCGGAISSQDVQSIIDSVVSMPNGTRLQILAPIIRDRK
                     GEYKKELTEMRNEGFVRARIDGVMLDLTEEISLKKQKRHTIEIVIDRFITRHGIEQKI
                     KSAIDMALKYSDTVIINLVKEQRDIPFSKAMACLQCGASFPEINPRLFSFNSTYGACP
                     QCHGLGITGTGTSEDAFGMSLQEGIGEDLVSSTEPFKHCKSCGGMRLRKEALSIKIEG
                     RNIGEFSNLTVENALPFVSSLTLTQREKIIASRVLKEVKDRLSFLLKVGLGYLTLDRM
                     SLTLSGGEAQRIRLATQMGSSLTGVLYVLDEPSIGLHPRDCTKLLESLSSLRDAGNTV
                     IVVEHDEETIKWADHIIDTGPGAGARGGWIVAAGTLSDIESNERSLTGRYLKGELSIP
                     LPGRRRRCSEFIEIIGAGEFNLKDIDVAIPLGALTCVTGVSGSGKSTLIVEILYKALM
                     KAIHSSAPIPGKHHAIKGIEKIDSVICVDQSPLGRTPRSNPATYTGVFGPIRELYAQV
                     QESRVRGFSSSRFSFNVAGGRCEKCHGGGILKVAMHFLPDAYVQCDTCKGKRYNRETL
                     AVQYRGKNISEILTMTVSEAMDFFGAIPVIKQKLEILEDVGLGYLQLGQPAPTLSGGE
                     AQRLRLSKELAKRSTGNTLYILDEPTTGLHFVDIQRLLDVLNTLVDKGNTVIVIEHNL
                     DVVKSADYLIDLGPEGGERGGHLIAAGTPEDISRNPDSSTGRFLKSKLSRSTSKVA"
     gene            3267..4178
                     /locus_tag="VMU21_06775"
     CDS             3267..4178
                     /locus_tag="VMU21_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUO77265.1"
                     /translation="MDTLVTITIVTDSADRAEKAMDKAFAEIGKLDTLLNFFSEKSEL
                     SMVNKNAGLSEVKVSPETMEVVEKAIETSEKTGGAFDVTIGPEISQWNFVLKIKPQDE
                     SIKRRLRLVNYKLIQLDKGRSTVHLKEKGMLMDLGGIAKGYAADKAVDELKKIGITSG
                     LVAVAGEVKAFGRKPGGKGWTVGIRNPRSTEKSDEIMATVQLNDAAISTSGDYQRYFI
                     LDGKRFHHILDPETGYPAQGCQSVSVIARDGVYTDAFSTGVFVLGPKKGIELLQSMGF
                     EGLIVDKDGTIYTTPGLKGKIEFTTNH"
     gene            4156..4548
                     /locus_tag="VMU21_06780"
     CDS             4156..4548
                     /locus_tag="VMU21_06780"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HUO77266.1"
                     /translation="MSLRQTISSTTLGDRILLVFLLLASFAGIVFIKDVLPSSGGVSI
                     EVQGKTTHRYLLNQNRTVRVESPYGHLTVEIRERKVRVINASCQNKLCEHQGWVTRGV
                     IICLPARISVSVGGPEKSGDKRIDATTG"
     gene            4532..5056
                     /locus_tag="VMU21_06785"
     CDS             4532..5056
                     /locus_tag="VMU21_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HUO77267.1"
                     /translation="MRPQDKYRIALLSAYALALHAFESLIPMPIPWLRVGLANIITIT
                     TFILFGFRSAMMVTLVRVIIASLFSGSFLGPGFILSMGGGLSSILAMGFMLSLVPRLF
                     GPVGLSLIGALSHNLSQLLLAYLLFIQKIEPVLLITPFLLLIGTITGTVNGLAADLLI
                     KGLKNSQQRIQNSR"
     gene            5056..5238
                     /locus_tag="VMU21_06790"
     CDS             5056..5238
                     /locus_tag="VMU21_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HUO77268.1"
                     /translation="MKIFCPLCKKATTWEENPWRPFCSERCKLIDLGKWASEEYKIPA
                     ENHEEEETHDQGKGEN"
     gene            5394..6197
                     /gene="dapB"
                     /locus_tag="VMU21_06795"
     CDS             5394..6197
                     /gene="dapB"
                     /locus_tag="VMU21_06795"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HUO77269.1"
                     /translation="MVRIAVAGAMGRMGSRIASLSEEYESVRLTAAFERKGHKDIGKD
                     IGGIYGMGDTGIILQDSMEKVIEMADVVIDFTHTSSTLENLKVASSKGKAMVVGTTGF
                     TKEQLAEIWTLTRNIPCVMASNMSLGVNLLLKILQDVAKVLGDDYDIEIIEAHHRMKK
                     DAPSGTAIKMAQVIADAVNRKLDEVAVYARKGMIGERTKKEIGIQTVRAGDIVGEHTV
                     LFGGLGERIEITHKASSRDTFARGALKAALWVYNKPAGLYDMQDVLGLK"
     gene            complement(6549..7376)
                     /locus_tag="VMU21_06800"
     CDS             complement(6549..7376)
                     /locus_tag="VMU21_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HUO77270.1"
                     /translation="MYIMRVLDLLERLEAKISELYEWFSRIYAQDAEAASLFYRISID
                     ETAHANLVKYEKRLVAQNTKIFGEISLDPGVINATLEMVTSVLSAASPSLEQAVKVSL
                     DIEHSAAEAHYRAAIAQASPDISRLLENLEGFDTRHIEVFEEFAANRGYPFVSKSKPS
                     PDRQVNPDRPVIKPDKTRIPIPPKLLEKIQYYYDLQKSMNFYKLLGIYDFASNEEIKK
                     AFHSFAREFHPDIYMNASEEVQQQLHTIFAHATDAYSTLMDPVKRRNYDMTRPRPRK"
     gene            complement(8254..8697)
                     /locus_tag="VMU21_06805"
     CDS             complement(8254..8697)
                     /locus_tag="VMU21_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77271.1"
                     /translation="MNQDITYRILIFDDDAFIRDVLWRIFNKRGFEVFTFPHPRSCPI
                     CEVLACTCALNEACADLIITDLNMPFMRGLDFLEQQKSKGCKVKNLALMAAALQEEDR
                     ARATELGIRVFEKPFQLADIKKWAAEAEAKISPRRGLTNWHLGHR"
     gene            complement(8766..>9356)
                     /locus_tag="VMU21_06810"
     CDS             complement(8766..>9356)
                     /locus_tag="VMU21_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HUO77272.1"
                     /translation="YRFPNSALITGLIIAALIEPELENRFIYLIAPALAILSKHLIRI
                     SGRNVFNPAAFGLLSLRLFYPEGFIVWWATTNIYLIIPLGLCIVLRMKGWHLVLSFML
                     ATVPLYVIYGIHSHLNLVDSLNLINPFFVLFVLTEHKSAPVANKARIAYGCVVGVLSF
                     LFHVFVPQVENSLTALLIGNVCAPIINRGFKTNGTH"
BASE COUNT         2534 a         2211 c         2347 g         2264 t
ORIGIN      
        1 aacacgaatc attcacagag cttctcctgg cagtcttcca ccttgtggag gaactggtgg
       61 tgagagaaga cgtcacctcc cttccggatg cagaccggca ccatatcgcg ggtgatataa
      121 agcgtgctta tatccttttg gtacaacaat ggctggaata tatgggacat cttagggacc
      181 attatcccta cctcttttcc ctggccataa ggataaaccc ttttgatgaa aatgcgtccc
      241 ctattgtaaa atcctgatac ctgcgttttc ggagaacatc cataaagaga ccttaagttc
      301 acaatccttg tatcaatacg caatacgcca caataacgcc ctcatttgaa agcgccttat
      361 tttttggggt atagtaataa gttagccttg ctttggctat taaatgtagc aatgcaataa
      421 gtacttatgg attcagattc agataagaga atttcaagcc cttaccaatg cgcagaggag
      481 gaggggtccc tgcaactgaa ttccggatcc ccgcatcaaa tgagtgcaac ctgctgaaag
      541 gagaaccacc caaaggtgca ggaccatata atcataaagg gggcgcgtga gcacaatctc
      601 aagaacattg atctctccat tccaagggaa aagattacgg tgatcaccgg tccctcagga
      661 tcagggaaat cctccctcgc catagacact atctacgcag aaggacagcg gagatatgtg
      721 gaaagcctct ccgcatacgc cagacaattc cttgaacaac tccagaaacc ggatgtggat
      781 tccatagaag ggctctcgcc ttcgatcgcg atcaaccaga agaccgtctc cagaagcccc
      841 cgctctaccg tgggtaccat aaccgagatc tatgattaca tgcgtgtcct ctatacgagg
      901 ataggcagac ccttctgttt tctttgcggg ggagcgattt cctcccagga tgtccagagc
      961 attatcgatt cggtcgtctc catgcccaac gggacaaggc tccagatcct cgcacccatt
     1021 atacgggaca gaaagggaga gtataagaag gagctgacgg agatgcgaaa cgaggggttc
     1081 gtgagggcaa ggatcgatgg agtgatgctt gacctgactg aagagatatc ccttaagaag
     1141 caaaaaaggc acaccataga gatcgtgatc gaccggttca tcacaagaca cggcatagaa
     1201 caaaagataa aaagcgccat agatatggcc cttaaatact ctgacactgt catcattaac
     1261 ctcgtgaagg aacagaggga cattccattc agcaaggcca tggcctgtct ccagtgcggc
     1321 gcaagttttc cggagataaa cccaagactt ttttccttta acagcaccta cggcgcatgc
     1381 ccccagtgtc atggcctcgg tatcaccgga acagggacca gtgaagacgc tttcggcatg
     1441 tccctgcagg agggaattgg ggaagacctc gtctcttcca cagaaccctt caaacactgc
     1501 aaatcatgtg gcggtatgag attgcggaaa gaagcactca gtataaaaat agaggggagg
     1561 aacatcggag agttttcaaa ccttaccgtg gagaacgccc tcccctttgt gagcagtctc
     1621 accctgaccc aaagggagaa gatcatcgcc tcccgcgtcc tcaaagaggt gaaagacaga
     1681 ctctcttttc ttctcaaggt gggcctcgga tacctcaccc ttgacaggat gtccctcacc
     1741 ctttcaggcg gcgaggccca gagaatacgc ctcgcgaccc agatgggctc ttccctcacc
     1801 ggagtgctct atgtcctgga cgaaccgagt attgggctcc atccgcggga ctgcacaaag
     1861 ctgctcgaaa gcctctcttc actaagggat gcgggaaaca ccgtgattgt ggtggagcat
     1921 gacgaggaga cgatcaaatg ggcagaccac attattgaca ccggccctgg agcaggggct
     1981 cggggcgggt ggatcgtggc agcagggacg ctatcagaca tagagtccaa tgaacgctcc
     2041 cttacaggca ggtatctgaa gggcgagctc agcatccccc ttcccggaag aagaagaagg
     2101 tgttcggaat tcatcgagat cataggtgcg ggagaattta atcttaaaga tatcgacgtt
     2161 gccatccccc tgggtgcgct cacctgtgtg accggcgtct cgggctcagg gaagagtacc
     2221 ctcattgtcg aaatccttta caaggcattg atgaaggcca ttcactcaag cgcccccata
     2281 ccgggaaagc accatgccat caaagggata gaaaaaatag acagtgtcat atgtgtggac
     2341 cagtcacccc tgggaaggac accccgctca aaccctgcaa cgtataccgg tgtttttggc
     2401 cccatcaggg aattgtatgc gcaggttcag gaatcgaggg tgaggggctt ttcttcatcc
     2461 aggttcagct tcaatgtggc aggagggagg tgtgagaagt gtcacggcgg gggaattttg
     2521 aaagtggcca tgcacttcct tcccgatgcg tatgtgcagt gtgatacctg taagggaaag
     2581 aggtacaata gagagaccct tgcggtgcaa tacagaggca agaatatttc ggagatcctc
     2641 accatgacag tatccgaagc tatggacttc tttggtgcaa tccctgttat aaagcagaaa
     2701 ctggagatac tcgaggacgt cggtcttggc tatcttcagc tggggcagcc cgcgcccacg
     2761 ctttccgggg gtgaagcgca gaggttgcgc ctctccaagg agcttgcaaa gaggtcaacc
     2821 ggcaataccc tctacatttt ggatgagccc accacagggc tccacttcgt cgatatccag
     2881 agacttcttg atgtgctgaa caccctagtg gataagggaa acacagtgat tgtgatagaa
     2941 cataacctgg atgtggtgaa atccgcggat tacctaattg accttggccc tgaaggcggg
     3001 gaaaggggtg ggcatctgat agccgcaggg acccctgaag atatttcaag gaatcctgac
     3061 tcctccacag ggaggttcct caaatcaaag ctctcccgca gcacgtcaaa agtggcatga
     3121 caatggcatt taaagctcaa aggtcgcaat tccgcgttca gaaaagaatc tcttgctgca
     3181 tattgttcac cttttcctgc ctgtgcattt ctctattttc ctgttcgtcc ccaaaagaaa
     3241 caatttatcg gaaaagcatg gtcctcatgg acacgctggt caccattacc atagtcactg
     3301 attcggcgga cagggctgaa aaggccatgg acaaggcatt tgccgagatc ggcaagcttg
     3361 atacactcct caatttcttt tccgaaaaaa gcgaactttc catggtcaac aaaaatgccg
     3421 gcctctccga ggtcaaggtc tccccggaaa ccatggaggt ggtggaaaag gcaatagaga
     3481 catcggaaaa gacagggggt gcgtttgatg tcactattgg acccgagata tcccaatgga
     3541 atttcgttct caaaataaag ccccaagatg aatcaataaa gaggcggctg aggcttgtaa
     3601 attacaaatt gatacaattg gacaagggga gatccactgt gcaccttaag gaaaaaggaa
     3661 tgctcatgga tctgggtggc attgcaaagg gatacgctgc ggacaaggca gtggatgagt
     3721 tgaaaaagat tggaataacc tccggtcttg tagccgttgc cggcgaagta aaggccttcg
     3781 ggcgaaagcc cggcggcaag gggtggacgg tggggataag gaaccccaga tccacagaaa
     3841 agagtgacga aattatggca acggtccaac tcaacgacgc ggctatctct acttcaggtg
     3901 actaccagag atactttatt ttggatggga agcgttttca ccatatcctc gatccggaga
     3961 cagggtaccc tgcccaggga tgccaaagcg tgagtgtcat tgcaagagat ggcgtgtaca
     4021 ccgatgcctt ctccaccggg gtctttgtcc tcggaccgaa aaaagggatc gagcttctcc
     4081 aaagcatggg ctttgagggc ctgatcgtgg acaaagacgg taccatttac actactcctg
     4141 gattgaaggg gaaaattgag tttacgacaa accattagct ccacgaccct gggagacagg
     4201 atactactcg tattccttct cctcgcatca tttgcaggca tcgtctttat caaggatgtt
     4261 cttccaagct ccggaggggt gagcatagaa gtccagggga aaaccaccca tcggtatttg
     4321 ctcaaccaga acaggacggt aagggtcgaa agtccgtacg gacatctcac cgtggagatt
     4381 agagaaagga aggtcagggt gataaacgca tcctgtcaga acaagctatg tgagcatcag
     4441 ggatgggtca cccgtggggt aatcatctgc ctgcccgcca gaatttcggt aagcgtcgga
     4501 ggtcctgaaa aatcggggga caagagaatt gatgcgacca caggataaat accgcatcgc
     4561 tctcctttcc gcctatgccc ttgcccttca cgcattcgag agcctgatcc ctatgccaat
     4621 accatggctc agggtcggtc ttgcaaacat catcaccatc accaccttta tcctgtttgg
     4681 attcaggtca gcaatgatgg taacgctggt acgggttatt attgcctccc tcttttcagg
     4741 ttcattcctt ggtcccggct ttatcctaag catgggagga ggtctctcga gcatacttgc
     4801 catgggcttt atgctctctc tcgttccacg gctgttcgga ccggtgggcc tcagtctcat
     4861 cggagcactc tcccacaacc tctcacaact actccttgca tacctcctgt tcattcagaa
     4921 gatagagccg gtcctcctca ttaccccttt tctcctcctt atcggaacca tcacaggaac
     4981 ggtgaacggg cttgcggcag atcttctcat caaaggcttg aaaaatagcc agcagaggat
     5041 tcagaatagc cggtaatgaa aatcttctgc cccctgtgca aaaaggcaac cacgtgggag
     5101 gaaaatccct ggagaccatt ctgttccgag aggtgcaagc tcatagacct aggaaagtgg
     5161 gcatctgagg aatacaaaat accggcagag aaccatgaag aggaggagac gcatgaccag
     5221 gggaagggag aaaattaggc attacgctcc ctcccctctt ttcgatctct catgtccact
     5281 ctgatcctgt cgcagcaata gtcatggaat ttgtcctgga atattgttaa aatctacccg
     5341 tcacgcatgg cttgggaata accaatgagt gtgagaactg aacaggaggt tatatggtac
     5401 ggatagcggt agcaggtgct atgggtagga tggggagcag gattgcctct ctctcggagg
     5461 agtacgagtc cgtcagactc acggccgcct ttgaaagaaa ggggcataaa gatatcggaa
     5521 aggatatcgg aggcatttac ggaatgggcg acacaggtat catcctgcag gacagtatgg
     5581 aaaaggtcat tgagatggct gatgtggtca ttgactttac ccacaccagt tcgaccctgg
     5641 agaatctgaa ggtcgcctct tcaaaaggaa aggcaatggt tgtgggaacc acaggcttta
     5701 ccaaagagca gttggcagaa atatggacgc tcaccaggaa tatcccctgt gtgatggcat
     5761 cgaatatgag cctgggtgtc aatctcctgc tcaaaatcct gcaggacgtg gcaaaggtcc
     5821 ttggcgacga ctacgacatt gagatcatcg aggcgcacca caggatgaaa aaggatgcgc
     5881 ccagcggtac tgccataaag atggcccagg tgattgcgga tgcggtaaac agaaagctgg
     5941 atgaagtggc ggtctatgcg agaaagggga tgataggaga gaggacaaaa aaggagattg
     6001 ggatccagac cgtccgtgcg ggagacattg tgggggaaca cactgtcctc ttcgggggtc
     6061 ttggcgagag gattgagata acccacaagg catccagcag agacaccttt gcgcgggggg
     6121 cattgaaagc ggcactctgg gtgtataaca agcccgcagg cctctacgac atgcaggatg
     6181 tgctggggtt aaagtaatta aggatttctc acaggactaa acaccagcct catttcttcg
     6241 ttaccaagga cttcgccccg tacgttgaaa ggcaacgatt tgacgtaggc ctgtacgtct
     6301 gagcagaaga ttctgtatcg tagattaatt ccgtgtgtaa tgccaagcga aacctttatt
     6361 ggacgaacta tcaagagaca ctgaaagtgg ttgtccgcgg ttgacatcaa gtccacctgc
     6421 aaagccgctc ctcggacatt tagcgatggt attgatttcg ggtaacaacg gtaagcttcc
     6481 ggtaatatct cgacccggaa gtctgcatgt gagttacgcg atcctattct gaacaggata
     6541 agtgcgggct atttccttgg ccttggccgt gtcatatcgt agttccttct tttgacagga
     6601 tccatgagcg tggaataggc atcggtcgca tgggcgaata tagtgtggag ttgctgctga
     6661 acctcttccg aagcgttcat ataaatgtca gggtggaact cacgcgcgaa agagtgaaat
     6721 gcttttttga tctcttcatt tgacgcaaaa tcatatatac cgaggagttt ataaaaattc
     6781 atagattttt gcaaatcgta gtaatattgt atcttctcaa gcaatttggg cggtatcggg
     6841 atcctggtct tatcgggctt aataaccggt ctgtcaggat ttacctgtct atctggagac
     6901 ggtttggatt ttgatacaaa ggggtatccc ctattggccg cgaattcttc aaagacttcg
     6961 atgtgtctgg tgtcaaagcc ttcgaggttc tctaggaggc gggatatgtc tggagaggcc
     7021 tgtgcaatgg cagcacggta atgggcctcg gcagcgctgt gttcgatgtc caacgatacc
     7081 ttcactgcct gctcaagtga gggcgaagca gctgagagaa cagatgttac catttcaagc
     7141 gtggcattga taactccggg gtcaagggaa atctcgccga aaatcttggt attttgcgca
     7201 actagccgct tttcatactt cacaaggttg gcgtgtgcgg tctcatcaat gcttatacgg
     7261 taaaagaggg aagcagcttc agcatcttga gcataaatcc tactgaacca ttcatagagt
     7321 tctgatatct tggcttccag tctttcaagg agatcgagta ccctcattat atacatcgcg
     7381 ctacctccag aacaatatag ttcaggcgag accctggagg gtagtttacc ataatgagat
     7441 ttcggctaaa agaacagttg gcagagacaa tggttggtac aggtaataat acggcagagg
     7501 caggggacag gagatattcc ttatctatcg aaaatggaaa ccggtgaagc ctgctgtgag
     7561 aaaggttgtg acgaacatct gtttcatgca agcaagcatt tcatcccata ctatgctcca
     7621 agaagacctt tgggaagcgt ccaacaaatt gttcaccttc ttcctgtatc aagcacacct
     7681 aatttcccta cgcaagtaaa gaatacaact gaaaatcatc acaataattc aagtcatcga
     7741 ctctgagggt aaaagagcat tgtgaagata gtaggctgcg atagtaacgt atcttattta
     7801 taatagctta tggatattga ggtcattgaa atctataaac tcttgccaag gatcgattgt
     7861 ggacagtgtt cttcaaagac ctgcatggct tttgccaaag caataaagga agactgcggg
     7921 gtactttctg aatgtgtgcg cctcaatgaa tctggtctca tgatgattga ggctttgttc
     7981 tgtcgcggcc aatgactaat ttgtcatgtc cgtttactgc ttctataatc gtttttcatg
     8041 ccttgccatc aactcatgcc gtcatgatta gtttcccctg ttttttttgc gataatttgc
     8101 gatccgtact gcctacactc atagatagat tccctttcga ctgccatgga aagctccttt
     8161 ttttaattgc tttaattata gtcacagaga tttgttaaaa tatttctaat gactcttttt
     8221 catgcagttg tcaacaacga cttgtcagcg ttcttagcgg tgtcccagat gccaattggt
     8281 aagccctcgt ctcggtgaga tcttagcttc tgcctcggca gcccatttct ttatgtctgc
     8341 aagttggaat ggcttctcga ataccctgat tccaagttct gttgctctgg ctctgtcctc
     8401 ctcttgaagc gctgcggcca ttaatgcaag attcttcacc ttgcatccct tagatttttg
     8461 ttgctctaag aaatctagcc ctctcatgaa gggcatattg aggtcagtaa taatcaaatc
     8521 cgcacaggct tcgttgagag cgcatgtaca tgcaaggacc tcacagatcg gacaggagcg
     8581 cggatgtgga aatgtaaaca cttcaaaacc tctcttgttg aaaatgcgcc acaacacatc
     8641 cctgatgaat gcgtcgtcgt caaagatcag gattctataa gtgatgtctt gattcacttg
     8701 ccactttcct ttctaccaaa cattatttat tagctcctcc tttcttaatt tattggtaat
     8761 acgccttagt gtgtcccatt agtcttgaac cctctattga ttattggtgc acacacattg
     8821 ccgattaaga gggctgtaag ggagttctcc acctggggca caaacacgtg gaaaagaaac
     8881 gacaggaccc ctacaacaca cccgtacgcg attctcgctt tattcgccac cggggcggat
     8941 ttatgttctg taagcacaaa tagcacaaaa aatggattaa tcaagttaag actatcaacg
     9001 agattcaggt gactatgaat gccatagatg acatatagtg ggaccgtagc cagcataaaa
     9061 ctcaaaacga gatgccagcc tttcattcta agaacaatac acaacccaag gggaatgatt
     9121 aaataaatat tggttgtggc ccaccatacg atgaatcctt ccgggtaaaa gagtcgtagc
     9181 gacagtaacc cgaaggctgc aggattaaag acattacgcc ctgatatgcg aatgaggtgc
     9241 ttagaaagaa tggcaagtgc cggtgctatt agatagataa acctgttctc caattcgggc
     9301 tcaatcagag cggcaataat cagacccgtt ataagtgcac tatttggaaa cctgta
//
LOCUS       DATKOU010000150         6648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3282857, whole genome shotgun sequence.
ACCESSION   DATKOU010000150 DATKOU010000000
VERSION     DATKOU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282857"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="rplI"
                     /locus_tag="VMU21_06815"
     CDS             <1..379
                     /gene="rplI"
                     /locus_tag="VMU21_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HUO77273.1"
                     /translation="EGYGRNFLIPKKIAVEANIKNVRALGHEKKKIEEKVKKIKSGAQ
                     QLSERVSALTLTLHAKAGEEEKLFGSITTMDIAEALKKEGIDIERKRILLDEPIKRLG
                     SYSIHIKIHPELTTHLSVQVVAE"
     gene            481..1845
                     /gene="dnaB"
                     /locus_tag="VMU21_06820"
     CDS             481..1845
                     /gene="dnaB"
                     /locus_tag="VMU21_06820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HUO77274.1"
                     /translation="MRDTDLALDKVPPQNIEAEQSILGAVLLENEALSKTLEIISPED
                     FYRESHRKIFNAMLDLFEKNEAIDLITVTDGLKRSNDLDAVGGVTYLSSLVTHVPTAA
                     NVRYHSKIVKEKALLRALLRSATEIAARVYESSLEADEMVDQAERTIFDISDKRTKTS
                     FYTLKDVIKSSFEMIEHLYDKKEAITGIPSGFGDLDALTTGFQPGDLVVIGGRPSMGK
                     TALGLNIAQHVGLEMREPIAIFSLEMSKEQLALRMLCSEAMVDSNSVRKGFIRKEDWH
                     KLTSAAGKLAEAPIFIDDSSSITVLEMRAKARRLKMEHGGLSLVIVDYLQLMRSRGGF
                     ERREQEISEISRSLKGLAKELRVPVIALSQLNRGVEQRHDRRPTLADLRESGAIEQDA
                     DVIIFLYRDRVYNKENTSDKAEVIIAKQRNGPTDTVHLTFLSHCTRFVSHADTEYHES
                     EEVF"
     gene            1842..2447
                     /locus_tag="VMU21_06825"
     CDS             1842..2447
                     /locus_tag="VMU21_06825"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HUO77275.1"
                     /translation="MIGVILAGGENSRIPALKGFLSIEGKAIIERSIETLTGILGQTV
                     ISTNMPEKYFCFGLPLVGDIKREKGPIIGILSVLTATREDSIFVVACDMPFVSEKLIR
                     YMVASFEDKMKRNTHVDAVIPVFEGKTEPLFGVYSRTVMKTIEGMLHSGRKGLIAMLE
                     DMNVHYITDEEVRTTDPKGASFVNINTMEDYERIGGKTCLV"
     gene            2435..2650
                     /locus_tag="VMU21_06830"
     CDS             2435..2650
                     /locus_tag="VMU21_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUO77276.1"
                     /translation="MFGLGMQELMVILIIVLVLFGASRLPEIGKGIGQAIKNFKKATS
                     EPDEIDITPKKSVDEKKEEKGEESRKS"
     gene            2729..3841
                     /gene="alr"
                     /locus_tag="VMU21_06835"
     CDS             2729..3841
                     /gene="alr"
                     /locus_tag="VMU21_06835"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HUO77277.1"
                     /translation="MLRGAIAEIDLNAITHNLGIVKAITRDRPVIAVVKADAYGHGAV
                     EVSKRLEDEGIALLAVAFIDEGVKLREAGIRSPILVLFDKYDSAAFFEYHLIPAIHDL
                     QTARRLSEEARKRENPLDVHLKIDTGMGRLGLCDKGEMEQVMTISKMGYLNVRGLMSH
                     FSDSDISDKSFALAQIKRFQGVRELITRGTGSPLFCHMANSAATISLSDAYFDAVRPG
                     LMLYGYSPFGDKGALSSGNTLKPAMTVKAKILALRKFQKGVPVSYGRTFITTRESVVA
                     VLSVGYADGYSRHFSNAIDVIVKGKRVPVVGRVCMDLTMVDVTGIEGVEEGEEAVLLG
                     TQGSQEVNAWEMAGKAATIPYEILTALGNKSRRVYV"
     gene            3855..4283
                     /locus_tag="VMU21_06840"
     CDS             3855..4283
                     /locus_tag="VMU21_06840"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HUO77278.1"
                     /translation="MKDRTGKEVNGMRIPFLTQISWYLIFAMFIIGIAPRVDAAMAPS
                     SLIASSPFERAADLAEIQRVLEMKVVKERLENLGFSAEEVRARLDQLSDHQVHQFAQQ
                     IDDLRVGKDSAGAVIIVLIVIVLIIVAILYFTGRKVVVTQ"
     gene            4358..4897
                     /locus_tag="VMU21_06845"
     CDS             4358..4897
                     /locus_tag="VMU21_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HUO77279.1"
                     /translation="MQRYLYICCILLVSMSGCTTVNPVPDEQIKGDAHIIRNVPFHPQ
                     EDFQCGPASLAAVLNYWGISVTPEQVAKDIYSSSAKGTLNIDMLLYAQSRGLAASQYP
                     GGLDDLREKINSEEPLIVLVDYGFSIIQANHFMVIIGYNDRGVIANSGRDEMRFIPVE
                     DFLKSWEKTKFWTLLITRK"
     gene            5007..5501
                     /locus_tag="VMU21_06850"
     CDS             5007..5501
                     /locus_tag="VMU21_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77280.1"
                     /translation="MKDKRSKTFCGYFYFVVFLFLALCSLLFAGCSLPRIIVLEDPLS
                     PEEHLNLGVAYEKKGEWESAIKEYEAASKKLPMAYTYLGNVYFQKNEFQVAEEYYRKA
                     IKKDPENADAYNNLAWLYFTKKEQLEEAEQLALKAMELNPSKKDIYQDTLDQIKAART
                     HPSK"
     gene            complement(5512..5940)
                     /locus_tag="VMU21_06855"
     CDS             complement(5512..5940)
                     /locus_tag="VMU21_06855"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUO77281.1"
                     /translation="MPVRNAEAVWEGNLLKGKGKMKLGSGAFEGAYSFSSRFENGLGT
                     NPEELIGAAHAGCFSMALSLMLEQAGYTPERVHTTAKVHVDKVGDGFKITVIELETQG
                     KIPGINEKTFLEKAEAAKKGCPVSQALSGTEIRLKATLVK"
     gene            complement(6064..>6648)
                     /locus_tag="VMU21_06860"
     CDS             complement(6064..>6648)
                     /locus_tag="VMU21_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77282.1"
                     /translation="IMGARGLKSIKSFLLGSVTRSVAINSPMPILVTKPLKGERPERM
                     RVLFATDGSSSSLATAAFLGSLPFPESTELVLMNVAWSAASDIPTRFAMEVDDKIKEH
                     VARARSIEMEASENIIDQTKPYLGKRFTKITGTSKGGDPSTEIIGEAERINADLIAVG
                     SRGLQGVRGMLGSVSRRVLGHAHCPVLIGKERGG"
BASE COUNT         1755 a         1509 c         1786 g         1598 t
ORIGIN      
        1 cgaggggtat ggaaggaatt tccttatccc taaaaagatt gcagttgaag cgaatatcaa
       61 gaatgtcagg gccttgggac atgaaaagaa gaaaatagag gagaaggtaa agaaaatcaa
      121 gagtggagcg cagcagctgt ccgagagggt ttctgccttg accctcactt tgcacgcgaa
      181 agcaggagaa gaggaaaaac tcttcggttc cattaccaca atggacattg cagaagcgct
      241 caagaaggaa ggaattgaca ttgagagaaa aaggattctc cttgatgagc ctatcaagag
      301 acttggttcc tattctatac acataaagat tcatcccgaa ctcacgaccc atctgagtgt
      361 tcaggtagtt gcggagtgag gatacgccgg gaaccggtga gcatccgtgt ggcgcggctt
      421 gggccgtttg gcagttaatt gttttaactt tttcatgttc tgtcctgcat tggtggaaag
      481 atgagggata ccgatttagc ccttgacaag gttcctccac aaaatatcga ggccgaacaa
      541 tccatcctcg gggccgtcct cctggagaac gaggcccttt cgaagaccct cgaaatcatc
      601 tctcccgaag atttttacag ggaatcccat cggaagatat ttaatgcaat gcttgacttg
      661 ttcgagaaga acgaggcaat tgatctcatc actgttaccg atggccttaa gagaagcaat
      721 gacctggatg cagtgggggg cgtcacctac ctttccagcc tggtgaccca tgtccccacc
      781 gcagccaatg tgaggtacca ttcaaagatt gtcaaggaaa aggcccttct cagggcgctc
      841 ctcaggtctg ccaccgaaat cgctgcacgg gtgtatgaat cctccctcga ggctgatgaa
      901 atggtggatc aggcggaaag aacgatcttc gacatttccg ataaaaggac aaaaacatct
      961 ttttacaccc tcaaagatgt gataaaaagc agttttgaga tgatcgagca tctctacgac
     1021 aagaaagaag ccatcaccgg gattccctcg ggattcggtg acctggatgc gctcactaca
     1081 gggtttcagc cgggtgatct cgttgttatc ggcggaagac cgtccatggg caagacagcc
     1141 cttggcctca acatcgccca gcatgtaggg ctcgaaatga gggagcctat tgccatcttc
     1201 agcctcgaaa tgtcgaagga gcagctcgca ttgaggatgc tctgttccga ggcgatggtg
     1261 gattcaaaca gcgtgagaaa gggattcatc aggaaggagg actggcataa gttaaccagt
     1321 gcggcgggaa agcttgccga ggccccgatc tttatagacg actcctccag catcactgta
     1381 ctcgagatgc gggcaaaggc aaggcgtctc aagatggagc atggcggctt aagtcttgtg
     1441 atcgtcgatt acctgcagct gatgcggagc agaggcggat ttgaaaggcg ggagcaggag
     1501 atatcagaga tatcccgctc ccttaaggga ctggcaaagg aactgagggt tcccgtaatt
     1561 gcgttgagcc agcttaacag aggagtggaa cagaggcacg acagaaggcc taccctggcc
     1621 gacctcaggg aatcgggtgc cattgagcag gatgcagatg tgattatctt cctctacaga
     1681 gacagggtct ataacaagga aaatacctct gacaaagcag aagtgattat cgcaaagcag
     1741 cgtaacggtc ctacggatac cgtgcatctc acctttcttt cccattgcac gaggtttgtg
     1801 tcccatgcgg atacagagta tcacgaatcc gaggaggtct tttgatcggg gtaatcctcg
     1861 caggcggtga gaacagcaga atccctgccc ttaagggctt tctgagcatt gaaggaaagg
     1921 cgattatcga acggagcata gagaccctca ccgggatact cggacaaaca gtaataagta
     1981 cgaatatgcc cgagaaatac ttctgtttcg gtctccccct cgtcggggac atcaagaggg
     2041 aaaaggggcc gatcataggg atattgtcgg tcctcacagc aaccagagaa gattcgattt
     2101 ttgtggtcgc ctgcgatatg ccgtttgtca gtgagaagct catacgctac atggtggcgt
     2161 cttttgagga caagatgaaa cgaaacacac atgtggatgc ggtcattcct gtgtttgaag
     2221 gaaaaacaga gcctctcttt ggtgtgtaca gcagaactgt gatgaaaacc atagagggca
     2281 tgctccacag tgggaggaag gggttgattg caatgctgga agatatgaac gtgcattata
     2341 ttacagacga ggaagtgagg accacggacc cgaagggcgc ttcttttgta aatataaata
     2401 ccatggaaga ttatgaaaga attggaggga agacatgttt ggtttaggga tgcaggaact
     2461 tatggtgatc ctgatcatcg ttctcgttct ctttggcgct tcacggctgc ctgaaatcgg
     2521 caagggaatt ggtcaggcca tcaagaactt caagaaggca acatccgagc ctgatgagat
     2581 agacattacg ccgaaaaagt ctgtggacga aaagaaagag gagaagggtg aggaatccag
     2641 gaaatcgtga acttttggca ttggtgtggg acatcgaaga gcgatagggg gttcatgggg
     2701 caacgatgtt tctcctatga gttaggaagt gcttaggggg gctatagcag agatagacct
     2761 caatgccata acgcataacc tcggtatcgt gaaagccatt acgagggaca ggcctgtcat
     2821 tgcggtggta aaggcggatg cctatggaca tggtgctgtc gaagtctcaa agagacttga
     2881 ggatgagggg attgcactcc ttgcagtggc atttatcgac gagggagtaa aactcaggga
     2941 agcgggtatc cgttcaccta tactcgtcct gtttgacaaa tacgactctg ctgctttttt
     3001 tgaatatcat cttatccccg cgatccatga tctccagaca gcgagaaggc tgtcagaaga
     3061 agccagaaaa agagaaaatc ccctggatgt tcatctcaag attgacacag ggatgggaag
     3121 actcggtctt tgtgacaagg gtgagatgga acaggtcatg accatctcga aaatggggta
     3181 tctgaatgtg aggggtctca tgagccattt ctctgattct gatatttcgg ataagtcctt
     3241 cgcccttgcg cagataaaaa ggttccaagg ggtgagggag ttgatcacac gggggaccgg
     3301 tagccccctt ttctgtcata tggccaacag cgcagcaacg atatccctct cggatgcata
     3361 ttttgatgcg gtgagaccag gtcttatgct gtacggatac tcgccgtttg gagacaaagg
     3421 agcgctctct tccggaaaca cgctcaagcc cgcgatgact gtcaaggcaa agatactggc
     3481 cctgagaaaa tttcagaagg gagtcccggt gagctacgga aggaccttta ttaccacgag
     3541 ggaaagtgtg gtcgcggtgt tatcggttgg ctatgctgac ggatacagca gacatttctc
     3601 aaatgcaata gatgtcatag taaaggggaa aagggttccc gtggtgggga gggtgtgtat
     3661 ggacctgacc atggttgatg tcacagggat tgaaggggtg gaggagggcg aagaagcggt
     3721 acttctcggt acacagggta gccaagaggt gaacgcttgg gagatggcgg ggaaggcagc
     3781 aacaatcccc tatgagatcc tgactgcact gggaaacaag tctcgcaggg tttatgtata
     3841 atacaggagc tatagtgaaa gacagaacag gaaaggaggt gaacgggatg aggatacctt
     3901 tcttaacgca gatatcttgg tatctgattt ttgccatgtt tattataggc atcgcaccaa
     3961 gagtggatgc agccatggca ccatcctctc tcatcgcttc ttcccccttt gagagggctg
     4021 ctgaccttgc cgagatacag agggtgctgg agatgaaggt ggtgaaagag cgactggaga
     4081 accttgggtt cagcgctgag gaggtcaggg cgagacttga ccagcttagt gatcatcagg
     4141 tgcaccagtt tgcccagcag atagacgacc tgagagtcgg aaaggattct gccggtgccg
     4201 tgatcattgt gctcattgta attgtgctta taatagttgc tatcctttat ttcacgggac
     4261 gcaaggtcgt agtgacgcag tgatgaatgg ttatggcgaa tgcgtgaggg agagtagcag
     4321 gcctttctcc cctcacgcat aaccatttca agggtctatg caaagatacc tgtacatctg
     4381 ctgcatcctt cttgtgtcca tgtctggctg caccacggtc aatcctgtcc cagacgaaca
     4441 gataaaaggc gatgcgcata ttatacgcaa cgtccccttt catccccaag aagatttcca
     4501 atgcggccct gcgtctcttg ccgcggtgct aaattattgg ggcatttcgg tgaccccaga
     4561 gcaggtggca aaggatatat acagcagctc agcaaaaggc accctgaata tcgatatgct
     4621 tctctatgcg cagtcgcgag ggcttgcagc atcccaatac ccggggggac tcgatgatct
     4681 cagagagaag ataaactctg aagaacccct cattgttctc gtggattatg gtttttctat
     4741 aatccaggca aaccatttta tggttatcat cggctataat gatcgcggag taatcgccaa
     4801 ctcaggaaga gatgaaatga ggtttatccc tgtggaggac ttccttaaat catgggagaa
     4861 gacaaaattc tggacactcc tcataacgag aaagtgaata gtgagcagtc aatagtgaga
     4921 agtaaaaagc aggattctca actgcctgac gactcgacca atgacgactt aaggtacttg
     4981 agagcgatca gtgtgaatgg tgaaatgtga aggacaaaag gagtaaaaca ttttgcggtt
     5041 atttctattt tgtggttttt ctgttccttg ccctttgttc tttgctcttt gccggttgtt
     5101 cgttgccgag gattattgtc cttgaagacc cgctcagtcc ggaggagcac ctgaatcttg
     5161 gtgtggcata tgaaaaaaaa ggcgaatggg agagtgcgat caaggaatac gaggcggcat
     5221 caaagaaatt gccgatggca tatacctatc tcgggaatgt ctattttcag aagaatgagt
     5281 ttcaagtcgc tgaggaatac tacaggaagg cgataaagaa agaccctgaa aatgcagacg
     5341 cctataacaa ccttgcctgg ctctacttta caaagaagga acaacttgaa gaagcggagc
     5401 agcttgcatt aaaggcaatg gagctgaatc cttcaaaaaa ggatatctat caggataccc
     5461 tggaccagat taaggcagcg aggacgcatc cgtcgaaata actttctgag gctatttcac
     5521 caatgtagcc ttcagcctga tctcggtgcc gctcagggcc tgggatacgg gacacccttt
     5581 tttggcggcc tcggcctttt cgaggaatgt cttctcattg atgccgggaa ttttgccctg
     5641 ggtctccagt tcgatcacgg tgatcttgaa accgtctccc actttgtcaa catgcacctt
     5701 tgcagtggtg tgaacgcgtt ctggtgtgta gcctgcctgt tcgagcatta gggacagggc
     5761 catggaaaaa cagcctgcat gcgccgcgcc tatgagttcc tccggattgg taccaaggcc
     5821 gttctcgaag cgggaggaga aggaatacgc gccttcaaat gcaccgcttc caagcttcat
     5881 tttcccctta cctttgagca gattgccctc ccatactgct tctgcgttac gtaccggcat
     5941 gacgaacctc cttatacaga tatggtgaat agaatctcat acagagccct cttccttcat
     6001 attttagggc agatttcctc ccgccttttc tcattatcta gagaccattc aaccgggcaa
     6061 caatcagcct ccacgttctt tcccgattag cacggggcag tgggcatgcc cgaggaccct
     6121 cctggatacg cttcccagca tccccctcac tccctgtagg cccctgcttc ccaccgcaat
     6181 aagatcggcg tttatccttt ctgcttcacc gatgatctct gtagaggggt ctccgccctt
     6241 cgatgtccct gttatctttg taaatctctt gcccaggtac ggtttggtct gatcgatgat
     6301 attttcagat gcttccattt ctattgacct tgcccttgcc acatgttctt tgattttgtc
     6361 atccacctcc atggcaaatc gtgtagggat atcagaggct gctgaccatg caacattcat
     6421 gagcaccaac tcagtacttt cggggaaagg cagagaaccg aggaacgctg cggtcgcaag
     6481 ggaagaggaa gaaccgtcag tggcaaaaag aaccctcatc ctttccggtc tctccccttt
     6541 taggggtttg gttaccagaa tcggcattgg cgagttgatc gccactgacc ttgtcacact
     6601 tccgaggaga aacgacttaa tactcttcaa tccccttgct cccatgat
//
LOCUS       DATKOU010000151         6953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3285856, whole genome shotgun sequence.
ACCESSION   DATKOU010000151 DATKOU010000000
VERSION     DATKOU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3285856"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VMU21_06865"
     CDS             complement(<1..806)
                     /locus_tag="VMU21_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUO77283.1"
                     /translation="MAEERKTTVSEEYLRNIEKKVDSLKLLIDVSTLISSTLELDELM
                     PLVMEKAKNVMDAEACSILFYNRETNKLEFEVAMCDEDNKSEILKKTITLEMGQGIAG
                     WVAENQKPLVIEDVTKDSRFFQGADKMTGFITRAIIAVPLVGRGGLIGVAEIINPARK
                     DYDPEIFELLCRQFAIAIENARYHRDSIERERLKQQLEIASSLQQSFLPQSPILTKGS
                     ITVSAVNISATQVGGDIYDFIETADGKLGVLIGDVSGKGISAALYMAKFI"
     gene            910..2496
                     /locus_tag="VMU21_06870"
     CDS             910..2496
                     /locus_tag="VMU21_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO77284.1"
                     /translation="MKQVEPGDMPGSPLIDIRNVNVTLQGQRILTNVSWQLRKGEHWA
                     VMGANGAGKSTFLKLINGDLWPDHGSGGRRVYHLGGNVQESPVGVREDIAFISPETQD
                     AYVRNQWDLSGKEVIYTGFFHSVWLHQKPDAEQKGYAERVAHLLRLDELRDKRFLAMS
                     EGEARKTLIARALVCRPGILLLDEFVSGLDIPSRGKILRLMERIAQSGTQVICTIHRI
                     NELMPSISHVLFLKEGRILCQGKKEEVLTPENLSVAIKGDYSRSVWGKGRDVPGPVKH
                     PRRNVPYLVKIRNADVYIEGRKILEGINWQIRRDENWAILGRNGAGKTTLLKLIRGDL
                     HPAFGGEVRRFGGLKDLDIRDMKKRIGYVSSELQANYDYPVTGEEVVQSGFFSSIGLY
                     EEVTGRQERIVRKWLKFFRMEAYADKDVRSMSYGEFRKILIIRAMVNDPDMLILDEPC
                     SGLDISTRHDVLTTLEKLSKTRTRIILVTHHLEDLVPSVSHILLMDEGRVIARGKREV
                     ILRKRNLSALFGNVTVPVFG"
     gene            complement(2474..3319)
                     /locus_tag="VMU21_06875"
     CDS             complement(2474..3319)
                     /locus_tag="VMU21_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUO77285.1"
                     /translation="MIPSLKFGDFELYWLRGGRFALDGGAMFGVVPKALWTKKYPSDK
                     DNAIPMIAYPLLLRTPEALVLIESGLGNKLNEKQKKIFRVTEDWMVLDDLKEMSIARD
                     DIGYVILTHFDFDHAGGVIMQNGKGRNEITFPKARHIVQKDEWEDVMSPNIRSINTYW
                     PANNELLRGSNLLQLVEGDKEIVKGVKVMHKGGHTKGFQIVVMESKEETATLLCDLMP
                     THAHFNPLWLMAYDNFPLEAIKQKEELEKTYIGRDAWFTFYHDPFVLACKFDETGNIT
                     QKLAL"
     gene            complement(3546..4028)
                     /locus_tag="VMU21_06880"
     CDS             complement(3546..4028)
                     /locus_tag="VMU21_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HUO77286.1"
                     /translation="MDIFEFAMKMEKDGEGYYRELAKKSSTTGLSNIFTMLAKAEVVH
                     YEIFKKMKENEKVKVADTKILSQVKTVFETMKEEKDMESALTETELYHKAVEAEKRSR
                     EFYLSKAGEVKDADQKEIFHKIADEEKKHYLILQEIVDFVSSPQTWLENPEWYHLEEY
                     "
     gene            complement(4129..>6953)
                     /gene="nifJ"
                     /locus_tag="VMU21_06885"
     CDS             complement(4129..>6953)
                     /gene="nifJ"
                     /locus_tag="VMU21_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HUO77287.1"
                     /translation="RYRLFDYYGHPEAERVMVIMGSGGETVLSTINILNAKGEKLGLV
                     QVRLFRPFDTDAFAQALPSSVKSIAVLDRTKEPGAIGEPLYLDVRTAIGEAMEKGTAR
                     FNSYPKVVGGRYGLGSKEFTPAMAKAVLDNLSEERPRNHFTVGINDDVAGTSLRYDES
                     FTVEGKGAYRAMFYGLGSDGTVGANKNTIKIIGSETDNYAQGYFVYDSKKSGSITTSH
                     LRFGKEKILNPYLLSKADFIACHNPSFLEKYDMLSRAEHGATFLLTTSHKKNEVWDIL
                     PIEVQEQLIAKKMKFYIIDAISLAEEIGLGSRINMIMQTAFFVISGIIPKEEAIKAIK
                     TEIKKTYMKKGDKVLEMNYASVDKALQNIIEVPVPQKVTSSTRVKPPVPEDAPKFVRE
                     VTATIIAGKGDTLPVSAMPADGSWPTGTTQYEKRNIGIHIPVWEPDICIQCGQCSFVC
                     PHATIRIKDYDPALLKNAPPGFKSADAKGKEFEGLKFTVQVAPEDCTGCGSCVFVCPG
                     FKKDAEGKKIPGFKAINMELQEQLRAREVANYGFFLNLPETDPSRYNVSTVKGSQLVK
                     PLFEYSGACAGCGETPYLKLLTQLFGDRLLIANATGCSSIYGGNLPTTPYAKRADGRG
                     PAWSNSLFEDNAEFGLGMRLTADKFRAQALELMERLAQKPLYADARELFDSLRNADQT
                     TQEKIEEQRGRVETLKKRLSKDSSPDAKSLLSLTDYLVNKSIWAVGGDGWAYDIGYGG
                     LDQVLASGKNINILVLDTEVYSNTGGQMSKSTPLAATAQFAAGGKATPKKNIGLIMAT
                     YGNIYVAQIAFGANPAQTVKAFTEAEAYNGPSLIVAYSTCIAQGIDMSKGIEEQKRAV
                     ACSHWPLYRYNPELEAQGKNPLVIDSKEPTMSFEEYAYNENRYRTLKTTNPDVAAALM
                     KQAEADVKRRWKYLKHRASWSPKEQG"
BASE COUNT         1555 a         1740 c         1803 g         1855 t
ORIGIN      
        1 ctgatgaatt ttgccatata cagggctgct gatattccct tgcccgagac atcacctatg
       61 agaacaccca atttgccgtc tgcggtttct atgaaatcat agatatcgcc tcccacttgt
      121 gtggcggaga tattcactgc agagaccgtt atgctcccct ttgtgaggat gggagattgg
      181 ggaaggaagc tctgctggag ggaggaagca atctccaact gctgtttgag cctttccctc
      241 tcgatggagt ccctgtggta tcgtgcgttt tctatggcga tggcaaattg tctgcagagg
      301 agctcaaaga tctcggggtc gtagtccttc cttgcgggat tgatgatctc agcaacgccg
      361 atgagaccgc ccctgcccac aagggggacc gcaataatgg ccctggtgat gaaccccgtc
      421 atcttgtctg caccctggaa gaacctgctg tcttttgtga catcctcgat gacaaggggt
      481 ttttgatttt ccgcgaccca gcctgcgatg ccctgtccca tctcaagcgt tatggtcttc
      541 ttcaggatct cggacttgtt gtcttcatcg cacatggcca cctcgaactc aagcttgttc
      601 gtctccctgt tataaaaaag gatggaacat gcctccgcgt ccatcacatt ttttgccttt
      661 tccatgacga ggggcatgag ttcatctaac tcgagggttg aagatatgag cgtggaaacg
      721 tctatgagga gcttgaggct gtcgaccttt ttttcgatat tcctgaggta ctcctcagaa
      781 acggtggttt ttcgttcctc tgccatcgct cctattaaat accagatatg tgaggaattt
      841 tcaaggagaa aatggcagtt ccatgagtct gtctttgttt ttttgcagag cgcaggttaa
      901 gataaaccca tgaagcaggt ggaaccagga gacatgccgg gcagtcctct tatcgacatc
      961 agaaacgtca atgtcacgct ccaagggcaa aggatattga ccaacgtatc atggcagctg
     1021 agaaaaggtg aacactgggc agtgatggga gctaacggcg caggcaagtc cacattcttg
     1081 aagttaatta atggagatct gtggcctgac catgggagcg ggggccggag ggtctatcat
     1141 ttgggcggca atgtccagga aagcccggtg ggggtgagag aggacattgc gtttatttcc
     1201 cctgagacac aggatgcgta tgtgagaaat caatgggatt tgagcggaaa agaggttata
     1261 tacacagggt ttttccatag cgtctggctc catcagaaac ccgacgctga gcagaagggg
     1321 tatgcggagc gcgtcgccca tttgctgagg ttggacgagc tgagggataa gaggtttctc
     1381 gcaatgtcag agggtgaggc ccgtaagacc ctcatagcaa gggcattggt ttgccgtccc
     1441 gggattctgc tcctagatga atttgtaagc ggccttgata ttccttccag agggaaaata
     1501 ctgcgtctga tggaaaggat cgcacaaagc ggaacgcagg tcatatgcac tatccacagg
     1561 ataaacgagc ttatgccctc catctcccat gtccttttcc tgaaggaagg acggatcctt
     1621 tgccagggga aaaaggaaga agtgctcact ccggaaaatt tgtctgtggc aattaagggt
     1681 gattactccc gttctgtatg gggaaaagga agagatgttc cgggacccgt gaaacacccg
     1741 agacgtaatg tcccctatct ggtgaagatc aggaatgcgg atgtatacat cgagggaagg
     1801 aagatacttg agggaatcaa ctggcagata aggcgtgatg agaactgggc gattctgggc
     1861 aggaatggcg cagggaagac caccctcctc aaactgatac gcggggacct ccacccggcc
     1921 ttcgggggag aggtgcgcag attcggaggt ctgaaggacc tagatatccg ggatatgaaa
     1981 aagaggatcg gctatgtctc ttcagaactc caggcaaatt atgactaccc tgtgacagga
     2041 gaagaggtgg ttcagtccgg atttttttcc agcataggat tatatgagga agtgaccgga
     2101 aggcaggagc ggatcgtgcg gaagtggctg aagtttttcc gtatggaagc ttacgcggac
     2161 aaggatgtcc gcagcatgtc gtatggtgaa ttcaggaaga tactcataat aagggctatg
     2221 gtgaatgacc ctgatatgct catccttgat gagccatgca gtggtcttga tatatcaaca
     2281 agacatgatg tgctcaccac tcttgagaaa ctgtcaaaga caaggacgag aattattctg
     2341 gttacccatc accttgagga cctggtcccc tctgtgagcc atattttgct tatggatgag
     2401 ggaagggtta ttgccagagg aaagagggaa gtgatactca gaaaaaggaa cctgtctgca
     2461 ctctttggca atgttacagt gccagttttt gggtgatgtt tcctgtctcg tcaaacttgc
     2521 aggcaaggac gaaagggtca tggtagaagg tgaaccacgc atcccttccg atataggtct
     2581 tctccaattc ctctttctgc tttattgcct caaggggaaa gttgtcgtag gccatgagcc
     2641 agaggggatt gaagtgggca tgggtgggca tcaggtcaca gaggagagtg gctgtctctt
     2701 cctttgactc cattaccacg atctgaaagc ctttggtatg gcccccttta tgcatgacct
     2761 ttaccccttt gacgatctcc ttgtcccctt ccaccagttg aagcagattg cttcctctca
     2821 ggagttcgtt attcgcaggc cagtacgtat tgatcgagcg gatgttggga ctcatgacat
     2881 cctcccactc atccttctgg acaatgtggc gagccttcgg aaacgtgatc tcattcctgc
     2941 ctttgccgtt ttgcatgatt actcctccgg cgtggtcgaa atcgaaatgg gtgaggatca
     3001 catatccgat atcatccctc gcaatactca tctccttcag gtcatccagt accatccagt
     3061 cctctgttac cctgaatatc ttcttctgct tctcgttcag tttattcccc aggcctgatt
     3121 caatcagcac aagggcctcg ggcgttctga gaagaagcgg gtaggcgatc atgggaatcg
     3181 cattgtcttt gtcggaaggg tatttctttg tccagagggc cttgggaacc accccgaaca
     3241 tggcaccacc gtcaagggcg aatctccctc ccctgagcca atagagttca aaatctccga
     3301 atttaagaga aggtatcatg actattcctg ttcagactaa cccattctat ttatgagtac
     3361 tctcttgcag gagaggcatc cgtctctcgg catgacaatt acccttattg acaccaccgt
     3421 tgtgtgcgca cctaaaaaca gtctgctcct gttttctgtt aatggtccgc atgttcggac
     3481 cggcacataa ccctgagtct tgccgtccga gcggggtacc tctcttaagt ttatgaatta
     3541 gcttgctagt actcttccag gtggtaccat tcaggatttt cgagccaggt ctgggggctg
     3601 gaaacaaaat ccactatttc ctgcaatata aggtagtgct tcttctcttc atcagcgatc
     3661 ttgtggaaga tctctttttg atcagcgtcc ttcacctcac ctgctttgct gagatagaac
     3721 tccctggaac gcttttcagc ctccacggcc ttgtggtaaa gttcagtctc cgtaagagca
     3781 ctctccatat ctttttcttc tttcattgtt tcgaagacag tttttacctg cgataatatc
     3841 tttgtgtcgg caaccttcac cttctcattc tctttcatct tcttgaatat ctcataatgg
     3901 accacttcag cctttgcaag catggtaaaa atgttgctga gtccggttgt tgaggacttt
     3961 ttcgccagtt cacggtagta gccttcccca tccttttcca ttttcatcgc aaactcgaat
     4021 atatccatat atgcctcctc tttcttcata gatgcagttt tgggccgagg cctttgactg
     4081 cggaacgtct ctcaagaaac gttcactgca taaagataca gatgcgcgct acccttgctc
     4141 tttgggagac cacgaagccc tgtgctttag gtacttccat ctcctcttta catccgcctc
     4201 agcctgtttc ataagtgcgg ctgcgacgtc cgggttggtc gtcttcaatg tcctgtagcg
     4261 gttctcatta tatgcgtatt cttcaaagga catggtcggc tccttgctgt cgatcacaag
     4321 agggtttttt ccttgggcct cgagctcagg gttgtatcgg tagagaggcc agtggctgca
     4381 ggcaaccgcc cttttctgtt cttcaatccc cttgctcata tcgatgccct gcgcgatgca
     4441 ggtcgaatac gccacaatca gggagggccc gttatacgcc tctgcctcgg taaatgcctt
     4501 taccgtttgt gcagggttgg cgccaaaggc aatctgggca acgtatatgt ttccgtacgt
     4561 tgccataata agtccgatgt tctttttcgg ggtcgccttc ccgcccgcag caaactgggc
     4621 agtggcggca aggggagttg acttggacat ctgtccgcct gtgttggaat agacctctgt
     4681 gtcgaggacc aggatattaa tattcttccc tgatgcgagg acctggtcca gtcccccgta
     4741 tccgatgtcg tatgcccacc cgtctccgcc cactgcccaa atggatttat tcacgaggta
     4801 gtcggtgagg gagagaaggc tctttgcatc aggggaactg tccttcgaaa gccgcttctt
     4861 caaagtctcc acccgacccc gctgctcctc gatcttctcc tgtgtcgttt gatccgcatt
     4921 acggagtgaa tcgaagagct cccttgcatc ggcgtacagg ggcttttgcg caagcctctc
     4981 catgagctcc agggcctggg ccctgaactt atccgctgtc agccgcatcc cgagaccgaa
     5041 ttctgcgttg tcctcgaaca gggagttgga ccaggcaggc cctctgccgt ccgcccgttt
     5101 tgcataagga gtagtgggca gattgccgcc gtaaatggag gagcatccgg tggcattggc
     5161 gatgagcaac cggtctccga agagctgggt aaggagtttc aggtacggag tttccccgca
     5221 ccctgcacat gcgccgctat attcaaagag aggtttcacg agctggctgc ccttcactgt
     5281 gctaacattg tagcgggaag ggtctgtttc aggaaggttg aggaagaacc cgtaatttgc
     5341 cacctccctg gccctcaatt gttcctggag ttccatgttg atggccttga agccggggat
     5401 ttttttgccc tcagcatctt tcttaaaacc cggacagacg aagacgcagg agccgcagcc
     5461 agtgcagtcc tcgggcgcca cctggacggt gaacttcaga ccctcgaatt cttttccctt
     5521 ggcatctgct gacttgaagc ctggaggagc gttcttcaga agcgcagggt catagtcctt
     5581 aatccttatg gttgcatggg gacagacaaa ggagcactga ccgcactgga tgcagatatc
     5641 aggctcccat acaggaatat ggatgccgat attacgcttt tcatactgcg tagtccctgt
     5701 gggccatgag ccatcagcag gcatggccga aacaggcaag gtatcccctt tgccggcgat
     5761 tattgtcgcg gtcacctctc tgacaaattt tggcgcgtcc tcaggcactg gtggtttcac
     5821 acgcgtagaa ctcgtgacct tttggggaac gggaacctct atgatgttct ggagtgcctt
     5881 atctaccgaa gcatagttca tctcgaggac tttgtccccc ttcttcatat aggtcttctt
     5941 gatctctgtt ttaattgctt tgatcgcttc ctcttttgga ataattccgg aaatgacaaa
     6001 gaatgcggtc tgcatgatca tattgatcct gctcccgaga ccgatctcct cggcaaggga
     6061 tatggcgtcg ataatataaa acttcatctt ctttgcgatg agctgttcct gcacctctat
     6121 cggcaggatg tcccacacct catttttttt gtgtgacgta gtgaggagga aggtagcacc
     6181 atgttcggcc cgggagagca tgtcatattt ttcgaggaac gaggggttgt gacaggcgat
     6241 gaagtcggcc tttgagagga gatagggatt gaggatcttc tccttgccaa agcggagatg
     6301 ggaggtggtg atggaaccag actttttgga atcatagacg aaatacccct gggcataatt
     6361 gtcggtctcc gagccaatga tctttatggt attcttgttt gcgcccacag tgccatctga
     6421 gcccaggccg taaaacatcg cccggtacgc gccctttccc tctacggtga atgattcatc
     6481 atacctgagg ctcgtgcctg ccacatcatc gttgatacca accgtaaagt gatttctcgg
     6541 cctttcctct gagagattgt ccagtactgc ctttgccatg gcaggggtaa attcctttga
     6601 accgaggcca taacggcctc ccacgacttt agggtaggag ttgaaccgcg cggtgccctt
     6661 ttccatggcc tcgccaattg ctgtcctcac atcaaggtaa aggggttctc cgattgcgcc
     6721 gggttccttt gtgcgatcca gtaccgcgat ggatttcaca ctggagggaa gggcctgggc
     6781 aaaggcgtcg gtgtcaaacg gtctgaagag cctcacctga acgagaccca gcttctcgcc
     6841 ttttgcattc aggatattga tggtgctgag cacagtttca ccgcctgaac ccatgatcac
     6901 catcacgcgt tcagcctccg ggtggccgta ataatcaaaa agcctgtatc gtc
//
LOCUS       DATKOU010000152         6663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3669443, whole genome shotgun sequence.
ACCESSION   DATKOU010000152 DATKOU010000000
VERSION     DATKOU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669443"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1210
                     /gene="tuf"
                     /locus_tag="VMU21_06890"
     CDS             11..1210
                     /gene="tuf"
                     /locus_tag="VMU21_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HUO77288.1"
                     /translation="MAKAKFERVKPHVNVGTIGHVDHGKTTLTAAITKVLAIKGQATY
                     RAYDSIDNAPEEKARGITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYMNKTDMVDDPELLDLVELEVRELLSK
                     YQFPGDKIPIIKGSALKAMESGSTDFNAPEFKSIHELMDAVDSYIPTPERPIDKPFLM
                     PIEDVFSISGRGTVVTGRVERGIVKVGEEVEIVGLGATKKTVATGVEMFRKLLDEGRA
                     GDNIGVLLRGIGKDEVERGQVLAKPGSITPHTKFKGEVYILTKEEGGRHTPFFNGYRP
                     QFYFRTTDVTGVAHLPEGVEMVMPGDNISVVVELIGPIAMEKELRFAIREGGRTVGAG
                     VVTEVLE"
     gene            1221..1526
                     /gene="rpsJ"
                     /locus_tag="VMU21_06895"
     CDS             1221..1526
                     /gene="rpsJ"
                     /locus_tag="VMU21_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HUO77289.1"
                     /translation="MNQKIRIKLRAYDHRILDQSVKEIVETAQRTGARISGPVPLPTR
                     VSKYTVLRSPHVDKKSREQFEIRTHKRLIDIYDPTPQTVDALMKLELAAGVDVEIKL"
     gene            1523..2137
                     /gene="rplC"
                     /locus_tag="VMU21_06900"
     CDS             1523..2137
                     /gene="rplC"
                     /locus_tag="VMU21_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HUO77290.1"
                     /translation="MKGILGKKLGMTHIFMEDGRAIPVTVIEAGPCCVIQIKTKENDG
                     YEAVKVGFSEIRKEKKVSKPMSGVFKKAGVKPYRIIKEFKMGDLKVGDLVTVGMFSKG
                     DIVKVSGVSKGKGFQGVMKRHNYAGGPGSHGSMFNRAPGSIGASSYPSRVWKNKGLPG
                     HMGSEKTTVKNLRVVEVRPDQNILMVWGAVPGAAGTYIEIRKEE"
     gene            2139..2768
                     /gene="rplD"
                     /locus_tag="VMU21_06905"
     CDS             2139..2768
                     /gene="rplD"
                     /locus_tag="VMU21_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HUO77291.1"
                     /translation="MPEIELKDANNKGKGKITLPDEIFGLQGRKDILHSSVVNYLANQ
                     RQGTHATKTKGLVSGGGKKPWKQKHTGRARAGSNRSPLWRGGGIIFGPQPRDYSYKLP
                     RNVKKRALGEALSAKLSGGEVTVVESIAVEKPRTKEMVKILKNLDLEGKRVLIVLSQK
                     EETVLLSSRNIPGVKVTRAMDLNPYEILTHEKVLITKKALETLSEGWTQ"
     gene            2765..3055
                     /gene="rplW"
                     /locus_tag="VMU21_06910"
     CDS             2765..3055
                     /gene="rplW"
                     /locus_tag="VMU21_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HUO77292.1"
                     /translation="MKNIHTIIKKPLFTEKGARLKEAENKVLVEVARDANKLDIKKAI
                     EEIFKVKVEKVATVNTEGKWKKQGKSIGKRPDRKKAIVTIKKGEKLDFIEGA"
     gene            3055..3879
                     /gene="rplB"
                     /locus_tag="VMU21_06915"
     CDS             3055..3879
                     /gene="rplB"
                     /locus_tag="VMU21_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HUO77293.1"
                     /translation="MGVKKFNPTSPGRRFQTVHDFKEITTDKPYEPLLKAIKKTGGRN
                     NSGCVTSWQRGGGNKRRYRVIDFKRDKHGVPAVVETIEYDPNRSSRIALLKYKDGERR
                     YIIAPSGLNVGEQVFSGRGVEIKTGNALPLTEIPLGTFVHNIELQPGAGAKLVRSAGM
                     AAQLIAKADDYSQVKLSSGEVRLVPTLCMATIGQVSNPEHENISIGKAGRTRWLGKRP
                     HVRGVAMNPIDHPLGGGEGRSSGGRPPCSPWGKPEGVRTRHNKRTDKFIVKRRGGK"
     gene            3880..4161
                     /gene="rpsS"
                     /locus_tag="VMU21_06920"
     CDS             3880..4161
                     /gene="rpsS"
                     /locus_tag="VMU21_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HUO77294.1"
                     /translation="MPRSLKKGPFVDAKLMKKVTQMMDSGDKKIIKTWSRRSTIIPDF
                     IGFTFAVHNGKKFIPVYVTENMVGHKLGEFSPTRTFKGHSGSAKAEAKA"
     gene            4165..4497
                     /gene="rplV"
                     /locus_tag="VMU21_06925"
     CDS             4165..4497
                     /gene="rplV"
                     /locus_tag="VMU21_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HUO77295.1"
                     /translation="MDAKATLHYARITPRKARRVVDLIRGKKAGEALISLRFMPYRGS
                     QFIEKILKSAMSNAEQKKAVNPEEMVISKAFVNQGPVMKRMEPRAMGRANVIRKRSSH
                     ITLILSGE"
     gene            4511..5173
                     /gene="rpsC"
                     /locus_tag="VMU21_06930"
     CDS             4511..5173
                     /gene="rpsC"
                     /locus_tag="VMU21_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HUO77296.1"
                     /translation="MGQKTHPVGNRLGITRTWDSRWFLKRGYADQLIEDINVRKIIKA
                     KLFHAGVARIEIERMGQKIRVIIHTARPGIIIGKKGAEVEKLRKELEAATGREASIDI
                     KEIRKPEVDSQLVAENIALQLEKRVAFRRAMKKAVASAMRFGALGIKVACAGRLAGAE
                     IARAEWYREGRVPLHTFRADIDFGFTEAKTTYGLIGIKVWIYNGDVLPEVLKSREGQL
                     VA"
     gene            5198..5617
                     /gene="rplP"
                     /locus_tag="VMU21_06935"
     CDS             5198..5617
                     /gene="rplP"
                     /locus_tag="VMU21_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HUO77297.1"
                     /translation="MLMPKKVKFRKMQKGRMDGKAYRGSEISFGEYGLKAMEPGWVSS
                     RQIEAARIAITRHVKRGCKVWIRIFPDKPITKKPAETRMGKGKGSPEYWVAVVKSGRV
                     LYEMSGVTEEVAKAALRLASHKLPISTKFVKREETVV"
     gene            5614..5805
                     /gene="rpmC"
                     /locus_tag="VMU21_06940"
     CDS             5614..5805
                     /gene="rpmC"
                     /locus_tag="VMU21_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HUO77298.1"
                     /translation="MRPSTLTTLTVDELKQKEQELRRELFNLRFRLATGEVENPMRIR
                     AVRKDIARVLTAMTEKQRG"
     gene            5828..6070
                     /gene="rpsQ"
                     /locus_tag="VMU21_06945"
     CDS             5828..6070
                     /gene="rpsQ"
                     /locus_tag="VMU21_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HUO77299.1"
                     /translation="MPKKVYTGKVLSNKMDKTVVVAITRLYQHPVYKKTVKKVTKFKA
                     HDQDNNCKVGDTVSISETRPLSKEKKWIVLEILERA"
     gene            6084..6452
                     /gene="rplN"
                     /locus_tag="VMU21_06950"
     CDS             6084..6452
                     /gene="rplN"
                     /locus_tag="VMU21_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HUO77300.1"
                     /translation="MIQPRSILEVADNSGAKRVQCIKVMGGSHKRYARLGDIVVVSVK
                     EAIPESNIKKGEKAKAVVVRTRKEHRRPDGSYIRFDQNAVVLINPQNEPLGTRIFGPV
                     ARELRWKEFMKIISLAPEVL"
     gene            6465..>6663
                     /gene="rplX"
                     /locus_tag="VMU21_06955"
     CDS             6465..>6663
                     /gene="rplX"
                     /locus_tag="VMU21_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HUO77301.1"
                     /translation="MGLGIKKEDTVLVIAGREKGKKGRVLSVMPAKSSLIIEKINIIK
                     RHMKPSRKYAQGGIIEKEAPIH"
BASE COUNT         2024 a         1225 c         2091 g         1323 t
ORIGIN      
        1 aggagggaaa atggcaaagg cgaaatttga gagggtaaag cctcacgtaa acgtagggac
       61 gattgggcat gtagaccatg ggaagacgac attgacggcg gcgatcacga aggtattggc
      121 gatcaagggg caggcgacgt atcgtgcgta tgattcgata gacaatgcac ctgaggagaa
      181 ggccaggggg atcacgatcg cgacggcgca tgtagagtat gagacggaca agaggcacta
      241 tgcgcatgta gattgtcctg ggcacgcgga ctatgtaaag aacatgatca ctggggcagc
      301 gcagatggac ggggcgatac tggtagtgag tgcagcggac gggccgatgc cgcagacgag
      361 ggagcacata ttgttagcga ggcaggtagg ggtaccgtat atagtggtat acatgaacaa
      421 gaccgacatg gtggatgacc ccgagttatt ggatttagtg gagcttgagg tgagggagtt
      481 attgtcgaag tatcagtttc ctggtgacaa gataccgatc ataaagggaa gtgcgttgaa
      541 ggcgatggag tctgggagca cggattttaa tgcacccgag ttcaagagca tccatgaatt
      601 gatggatgcg gtggacagtt acataccgac gcctgagagg ccgatagaca agccgttctt
      661 gatgccgata gaggatgtat tttcgatatc tgggcgtggg acggtagtga cggggagagt
      721 agagaggggg atagtgaagg taggggagga agtagagatc gtggggttag gggcgacgaa
      781 gaagacggta gcgaccgggg tagagatgtt caggaagctc ttggatgagg gaagggcagg
      841 agacaatata ggggtattgt tgagggggat agggaaggac gaagtagagc gtgggcaggt
      901 attggcgaag cctgggagca tcacgccgca cacgaagttt aagggagaag tgtacatatt
      961 gacgaaggaa gagggaggca ggcacacgcc gtttttcaat ggataccggc cgcagtttta
     1021 tttcaggacg actgacgtga ccggagtggc gcatttacct gagggagtgg agatggtgat
     1081 gcctggggac aatatcagtg tagtggtgga gttgataggc cctatcgcga tggagaagga
     1141 gttgaggttt gccatcaggg aagggggaag gacggttggt gcaggcgtag tgaccgaggt
     1201 cctggagtga gaaaactacc atgaatcaga agataagaat taaattaagg gcttacgacc
     1261 acaggatcct tgaccagtcg gtgaaggaga tcgttgagac tgcccagaga acaggggcga
     1321 gaatttcggg gcctgttccc ttgcctacaa gagtgagtaa gtatacggtc ctgcgttcac
     1381 cccacgtgga caaaaagtcc agggagcagt ttgagataag aacccataaa aggttgatcg
     1441 atatctatga tcctactccc cagacagtgg atgcgttaat gaagctcgag cttgctgccg
     1501 gggtggatgt ggagataaag ttgtgaaagg gatactgggc aaaaaactgg ggatgaccca
     1561 catattcatg gaagacggaa gagcgattcc ggttacggtg atcgaagccg gaccctgttg
     1621 tgtgattcag atcaagacca aggaaaatga cggatatgag gcggtgaaag tcgggttttc
     1681 cgaaataagg aaagaaaaaa aggtgagtaa gccgatgtcc ggagttttta agaaggcagg
     1741 ggttaagcct tatcgcataa taaaagaatt caagatggga gatttgaagg tcggtgacct
     1801 tgttaccgtt gggatgttta gtaaaggcga cattgtgaag gtatcagggg tctcaaaagg
     1861 taaaggtttt cagggagtta tgaagagaca caattatgca ggaggtcccg gctcccatgg
     1921 ctctatgttt aaccgggcac caggttccat tggagcgagt tcataccctt caagggtgtg
     1981 gaagaacaag ggacttcccg ggcatatggg ctctgaaaaa acaacggtga agaacctcag
     2041 ggtggttgag gtaaggcctg atcaaaatat attaatggtg tggggtgcag ttcctggggc
     2101 tgcgggcacc tatatcgaga tcaggaagga agagtaaaat gccagaaata gaattaaaag
     2161 acgcaaacaa taaaggaaag ggcaagatta ccctccctga tgaaattttc gggcttcagg
     2221 gcaggaagga tattctccat tcatcggtgg ttaactacct tgcaaaccag agacagggaa
     2281 cccatgccac caagacaaaa ggtcttgtca gcgggggcgg caagaaaccg tggaagcaga
     2341 agcacacggg aagggcaagg gcaggaagca accgttcccc gctttggaga gggggaggaa
     2401 taatattcgg acctcagccc agggactatt cttataaatt gccaagaaac gtcaagaaaa
     2461 gggcattggg agaggcgctg tcagccaagc tttccggcgg cgaagtaacc gttgtggaga
     2521 gcattgcggt ggaaaagccg agaaccaagg agatggtgaa aattctgaag aaccttgacc
     2581 tggaagggaa acgcgtgctt atcgtgcttt ctcaaaaaga agagacggtc ctcctttctt
     2641 caaggaatat cccgggtgtg aaggtcacaa gggcgatgga ccttaatccc tacgagattc
     2701 tcacccatga gaaggttctc atcacgaaaa aagccctgga aactctctcg gaaggatgga
     2761 cgcagtgaag aatatccata ccattatcaa gaagcccctc tttacagaga agggtgcaag
     2821 actgaaagag gcggagaaca aggttcttgt ggaagtggca agggatgcga acaagctcga
     2881 catcaagaag gcaattgaag agatattcaa agtcaaggtc gaaaaggtgg caacagtgaa
     2941 cactgaggga aaatggaaga aacagggcaa gtccatcgga aagagacctg acaggaaaaa
     3001 ggcaattgtg acaattaaaa agggtgaaaa actcgatttc attgagggtg cataatgggt
     3061 gtaaagaaat ttaatccaac atctccgggg agaaggttcc aaacggtgca tgatttcaaa
     3121 gagatcacga ccgacaaacc ttacgagccg cttttgaagg caataaagaa gaccggaggc
     3181 aggaataact ccggatgtgt cacctcctgg cagaggggcg gaggcaataa gagacgttac
     3241 cgggtgatag atttcaaacg ggacaagcat ggggtgcctg ccgtggtgga aacaatagag
     3301 tacgacccaa acaggtcttc acggatagcc ctcctgaaat acaaggatgg ggagagaagg
     3361 tatattattg cgccctcggg gctgaatgtg ggggaacagg tcttttcggg acggggtgtg
     3421 gaaataaaga ccgggaatgc cctgccgctc acggagatac cccttggcac gtttgtccat
     3481 aatatagaat tgcagcctgg cgctggcgcg aagcttgtcc gcagcgctgg tatggctgct
     3541 cagctcatcg caaaagcaga tgattacagc caggtgaagc tctcctcagg cgaagtccgg
     3601 cttgttccta cgttatgcat ggcaaccata ggacaggtga gcaaccctga gcatgagaat
     3661 atttccatcg ggaaggcggg gaggacccgc tggctcggga agaggcccca tgtaaggggc
     3721 gttgcaatga acccgatcga tcatccgctg ggtgggggtg aggggaggag ttcgggagga
     3781 agacctccgt gctcaccatg gggaaaacct gaaggggtaa gaacaaggca taacaagaga
     3841 acagataaat ttattgtgaa gaggcgagga ggtaagtaaa tgccaaggtc cttaaaaaag
     3901 ggtcctttcg tggatgcaaa gctgatgaaa aaggtcacgc aaatgatgga ttccggagac
     3961 aagaagatca tcaagacatg gtcaaggcgc tccactatta tccctgactt catcgggttt
     4021 acctttgcgg tacacaacgg aaagaaattt atccctgtgt atgttaccga aaatatggta
     4081 gggcataaac tgggtgagtt ttcgcccacc cggactttta aaggccattc tggatctgca
     4141 aaggcggagg caaaggcata atatatggac gcgaaggcta cattacacta tgcaaggatt
     4201 accccgagaa aggcaaggag ggtggttgac cttatccgag ggaaaaaggc cggggaagca
     4261 ctcattagcc tcagattcat gccctacagg ggctctcaat ttattgagaa gatactgaag
     4321 tcagccatgt ccaacgcgga acagaaaaag gcagtgaatc ctgaagagat ggtgataagc
     4381 aaggcgtttg tgaaccaggg accggtaatg aaaagaatgg aaccaagggc tatgggaagg
     4441 gcaaacgtga taaggaagag atcatcccat atcacgctga ttttatcagg ggaataataa
     4501 ggaggtaagt ttgggccaga agacgcatcc ggtaggaaac aggttaggca ttacaaggac
     4561 atgggactca aggtggtttc tgaagagggg gtatgccgat cagctcatcg aggacatcaa
     4621 tgtacgaaag ataataaagg caaagctctt tcatgcggga gtggcccgta ttgaaataga
     4681 aagaatgggg cagaagatca gggtgattat ccatactgcg aggccgggta tcatcatcgg
     4741 caagaagggt gccgaggtgg agaaactgag gaaggaactg gaagccgcca ccggaaggga
     4801 agcctccatt gacattaagg agataagaaa acctgaggtg gactctcagc ttgtggcaga
     4861 gaatattgca ttacagcttg aaaagagagt tgccttcagg agggccatga aaaaggcggt
     4921 ggcatctgcc atgaggttcg gcgccctggg tataaaggtg gcgtgcgcag gacggcttgc
     4981 cggggctgag atcgcaaggg ctgaatggta cagggagggg agagtacctc ttcatacgtt
     5041 cagggctgac attgattttg gttttactga ggcaaagaca acctacggcc ttatagggat
     5101 taaagtctgg atatataacg gcgatgtcct ccctgaagtg ctcaagtcaa gggaaggtca
     5161 actggttgcc tgatgacgac aaaggagtga atgacagatg ttaatgccga aaaaagtcaa
     5221 attcaggaag atgcagaaag gcagaatgga cggcaaggcc tacaggggtt ctgaaatctc
     5281 ctttggtgag tatggactga aggccatgga gccgggatgg gtttcatcgc gccagataga
     5341 ggcagccaga atagccatca cacggcatgt gaaaagggga tgtaaggtgt ggataagaat
     5401 attccctgac aagcccatta caaaaaagcc tgcggaaaca agaatgggaa aagggaaggg
     5461 ttctcctgag tattgggtgg ctgtggtgaa gtccggcaga gtactctatg agatgtctgg
     5521 agtgaccgag gaagtggcaa aggctgccct aaggctggca tcacacaaac tccccatttc
     5581 tacgaaattt gtgaagaggg aggagacggt ggtatgagac cctcaaccct tacgacattg
     5641 actgtggacg aattgaagca aaaggagcag gaactaaggc gggaactgtt caatctcaga
     5701 ttccgtcttg ctaccggcga agtggaaaat cccatgcgta tccgtgcagt gcgaaaggat
     5761 atcgcacggg tcctcaccgc gatgacagaa aagcagcggg gataaggaag agaggaagac
     5821 aacagatatg ccgaagaaag tttatacagg aaaagtgtta agcaacaaaa tggataaaac
     5881 agtggttgtt gcgattacaa ggctctacca gcatcccgtg tataagaaaa cggtgaagaa
     5941 ggtgacaaag tttaaggcgc acgaccaaga caacaactgt aaagtgggag acaccgtgtc
     6001 cattagtgag acgaggcctc tgagtaaaga gaaaaagtgg attgttctgg agatattaga
     6061 acgggcgtga ggggtgagat aagatgatcc aaccaagaag catacttgag gtagctgaca
     6121 attctggagc aaagagggtg cagtgcataa aggtgatggg cggctctcac aagaggtatg
     6181 cgaggcttgg cgacatcgtc gtggtgagtg tgaaagaggc gattccggag agtaacatca
     6241 agaagggaga aaaggcaaag gcggtggtgg tgaggacaag aaaggagcac aggagacctg
     6301 atggatcata tatacggttt gaccagaacg cggttgtgct catcaatccg cagaatgagc
     6361 ccttaggcac caggatattc ggtcccgttg cgagggaact caggtggaag gaattcatga
     6421 agattatatc attggcacct gaggtgctct agtgaggaga taagatgggg ttagggatta
     6481 agaaggaaga tacggttttg gtaattgcag ggagagaaaa aggcaagaaa ggaagggtcc
     6541 tttctgttat gccggcgaag tcgtcgctta ttatagaaaa aataaatatt attaagaggc
     6601 atatgaagcc cagcaggaaa tatgcgcaag gaggcattat cgaaaaggaa gcacctatcc
     6661 ata
//
LOCUS       DATKOU010000153        19076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4443762, whole genome shotgun sequence.
ACCESSION   DATKOU010000153 DATKOU010000000
VERSION     DATKOU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 19076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19076
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4443762"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VMU21_06960"
     CDS             <1..1405
                     /locus_tag="VMU21_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HUO77302.1"
                     /translation="KFALVENFWETGFGMPSFTLLGPKIIRFPFIINSSYPHEILHNW
                     WGNSVFPDSAKGNWSEGLTAYLSDHLIKEQQGSGVEYRETTLQKYADYVVAGRDFPLV
                     DFRSRHSSPSEAVGYGKSLMFFHMLRRELGDRSFIAGLQDFYNKNKFSFASFDDLRTS
                     FEKASGKNLKGEFEQWIERPGAPEIRLSDAKSRPEGEGYLLTAVLEQIQEGKGYRLAV
                     PIVITMEAQEKAYETVVNMENRQLDLKISLPSRPLRIDVDPQFDLFRRLKRDEVPPAI
                     SQALGTKRMVVILPSSEKGHLLSAYRKLAKTLGSSGPDEVEVKLDTEMEKLPPDSAVT
                     IMGWENRFLNSIVSALSPHEVAINQKGIRIEKTEIPRENHSVVLTVRNPQNKDLCWMF
                     IASDKAEALRGLGRKLPHYHKYSYLGFEGDEPENIAKGRWPILDSPMTAIFSSKDGKL
                     VKVGMGMLAPRKPLAEQ"
     gene            complement(1465..1794)
                     /locus_tag="VMU21_06965"
     CDS             complement(1465..1794)
                     /locus_tag="VMU21_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77303.1"
                     /translation="MPRKLNCWEYMKCGRELNGKKVKELGVCPVAVHPYADGINEGIN
                     GGRICWAIVGSYSLYNLKDPCTGQSHLCFECDFHKKVLAEEGIIDPGQLPQKKKMKKP
                     KQSHSKK"
     gene            2005..2967
                     /locus_tag="VMU21_06970"
     CDS             2005..2967
                     /locus_tag="VMU21_06970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO77304.1"
                     /translation="MKTFIHLDGALGDVFLSLPCICVIRVNSDFVHMAGPPDVADLLK
                     EVGYADEITPSGSALFTSLYALEMEQGAMGFLEQFDHAVVFTRRRDSPLALNIEKVIP
                     HTEIIITIPPEGVKTHVAQFRLNQLALGVESVDLRSKLTIPSPYREKAKEFLVRYFGT
                     EGRGPLIAFHPGSGGKRKCWPLNNYFRLVEKLLNHLACRILFLTGPAEEPEVADEILS
                     FAHGHKGVLHVRNEPLIMVGCLLANSDLYVGNDSGITHLAAAVNGRVVVLFGPSDPLL
                     WRPFGTGVQVISAGISEDSLERVGVDEVYARLTSSLREATREYS"
     gene            complement(3005..3859)
                     /locus_tag="VMU21_06975"
     CDS             complement(3005..3859)
                     /locus_tag="VMU21_06975"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HUO77305.1"
                     /translation="MHVEPLDFSHRELLHERLRKLQCPIAEYSFPNLYLFRDAHRYEV
                     ILDKGIFIKGQSYDGYTFLMPTVEIEKMECGYINAMMSSVDFLFPIPETWLKCFSQDE
                     YQCTCKQGDMDYLYTVEQMSTYRGRNLHSKRNLLKQFISTYAHVALPLTNDRLNDALS
                     VLNGWLEDSEQDETQTDYSPCLEALRKYDDLILCGGIYYADNEPAGFIVGEELNEETF
                     ALHFAKARKRFKGIYQYMFNTFARILPPKYRFLNFEQDLDRDTLRLAKSSYQPHSMLK
                     KARVALKR"
     gene            complement(3878..4624)
                     /locus_tag="VMU21_06980"
     CDS             complement(3878..4624)
                     /locus_tag="VMU21_06980"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HUO77306.1"
                     /translation="MPRIFLPLEERPETIHIAGEKARYLINVLRSHVGDEIMVLDGKG
                     SSYNAEITSVKNRELFARVKGAVPYDTESKVNIILIQGVLKGEKMEFLIQKTTELGVN
                     EILPVTTERSQLRETRKGPRWKKIAEDASRQCGRTHVPRIHDLLPFRELFAPSSLYAQ
                     YFRNSRGLLFWEEGGMKLTKARDVLGGCRSLMISIGPEGGFTKEEAYMARSRGFLVTS
                     MGNRILRAETAAIAATALVQYVWGDLDTGI"
     gene            complement(4629..5726)
                     /gene="dnaJ"
                     /locus_tag="VMU21_06985"
     CDS             complement(4629..5726)
                     /gene="dnaJ"
                     /locus_tag="VMU21_06985"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUO77307.1"
                     /translation="MKDYYEILGVPRNATEAEIKKAFRGLALKHHPDRNQGNRDAEEK
                     FKVINEAYSVLSDPEKRAHYDRYGSAEDMGAGFGAFTGAGFGDIFDDIFGDIFGFSSQ
                     RRPRAAKGNDLRYDLDITLEDSAFGTEKTIEVPRWETCSSCSGSGSKPGKGPVTCTNC
                     KGTGHVRFQQGFFSVSKTCGSCHGTGKIITDPCPSCKGHGKVKKVHTISVRIPAGVDS
                     GSRLRMSGEGEPGIYGGPPGDLYIIIDIEEHPLFVRKGNTLYHETTITFPQAVLGAEI
                     EVPTLEGSAHLKVPPGTPPGKAFPLKGKGIPRLGGHGKGDEIIVVNIEIPKHITPRQR
                     ELLEEFAQIDGDKTSKTFTSKLKDIFSGAEK"
     gene            complement(5723..7633)
                     /gene="dnaK"
                     /locus_tag="VMU21_06990"
     CDS             complement(5723..7633)
                     /gene="dnaK"
                     /locus_tag="VMU21_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HUO77308.1"
                     /translation="MAKAIGIDLGTTNSVVAVVQGGEPVVIPNQEGTRTTPSVVAFTE
                     KGERLVGQIAKRQSITNPENTIYSIKRLMGRKYNTPQVDHAKKRLPYKIVEASNGDAH
                     VEIRGKNYSPPEISAMILQKLKQAAEDYLGEPVTDAVITVPAYFDDSQRQATKDAGKI
                     AGLNVLRIINEPTAASLAYGLDKKKEEKISVYDLGGGTFDISVLEIGEGVIEVKSTNG
                     DTYLGGDDFDLRVMDWMVEEFRKEQGIDLKQDRMALQRLKEAAERAKIELSTATETEI
                     NLPFITADATGPKHLLMKLSRAKLEQLTGDLIEKTIEPCKLALADAGLSTKDIDEVIL
                     VGGQTRTPKVQQVVQSFYGKEPNRTVNPDEVVAVGAAIQGAVLKGEVKEVLLLDVTPL
                     SLGIETLGGIFTKIIERNTTIPTKKSQIFSTASDNQPAVTIKIHQGEREMAADNKLLG
                     VFELVGIPPAPRGVPQVEVSFDIDANGILHVSAKDMGTGKEQSIRITASSGLSEDEIK
                     KMTRDAESHADEDKKKKQLAEAKNEADTLVYTVEKSLKEYGDKVTETEKREIEEALEK
                     CRKVKETSTDAAEIKSAVENLTKASYKIAEHIYKAAGAGAGAQTSGPETKAKPEEEVV
                     EAEFEDVDKKEK"
     gene            complement(7635..8264)
                     /gene="grpE"
                     /locus_tag="VMU21_06995"
     CDS             complement(7635..8264)
                     /gene="grpE"
                     /locus_tag="VMU21_06995"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HUO77309.1"
                     /translation="MTHSHILCPQSAEGANEEEMLENEEVTEKQEGETPLPTDSEKGG
                     EKQPLQESLTELNDKYLRLYAEFDNYKKRIQREKEDLIKYSNEDILYHLLPVMDNLEM
                     ALKHSADSVSEGLVKGVEITLREFQRVTEKFGLIPIPAKGKPFDPAVHHAMSQVERTD
                     MEDRTVVEEFRKGYMFSGKVLRPSLVSVSRRPVDETETKIRINENTKEE"
     gene            complement(8264..9289)
                     /gene="hrcA"
                     /locus_tag="VMU21_07000"
     CDS             complement(8264..9289)
                     /gene="hrcA"
                     /locus_tag="VMU21_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HUO77310.1"
                     /translation="MFDERIHKVLWAVVESYIINPDPVGSRFVTKKYAFNLSSATIRN
                     IMADLEDMGFLHQPHTSAGRVPTDKAYRLYVDYLTQKEGTPDATLMDEIIKRLENIRT
                     DINALLRETSRALSTFSHYLGVALPPNPNNTTLKGINLFKYKERQVMIVLITDEGIVK
                     NKVIQLDPDLTQHDLDRISGYLNSEFAGQTLDEIRSSVLREMHQDKALCDTLISRAIR
                     ICREALSFDSGDVFISGFHEVLGLPDFTDIQRIKEISKAIEDKHLIIKLLDMLPQSEG
                     VRVLIGSENPASEMKKLSMVLSSYREGDRPVGTIGIIGPTRMDYMKVITIVDTTAKFI
                     TKILSER"
     gene            complement(9382..10218)
                     /gene="speE"
                     /locus_tag="VMU21_07005"
     CDS             complement(9382..10218)
                     /gene="speE"
                     /locus_tag="VMU21_07005"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HUO77311.1"
                     /translation="MIKFFEKDPYAPIQYVYGVEKVLHKSKSPFQEIVVIENPYFGKM
                     LILDGVVQITERDEFFYHEMLAHVVLHSHPHPRNVVVIGGGDGGAVREILKHDTVRKV
                     YFIEIDEEVINVSRKFFPTVASHVGDTRVEIRCMDGAEFVKGRNSDIDAIIVDSTDIV
                     GFAKSLFTVEFFKSVKDCLTDEGMFVSLSESLHFHKDMVIDVQEAMRLIFPVVDLYTA
                     PLVTYAGNWWTFSVASKKHDPRKMKREFKIDTKYYSDEIHQQCFLPHKMYEKLMKRKL
                     QW"
     gene            complement(10308..11441)
                     /locus_tag="VMU21_07010"
     CDS             complement(10308..11441)
                     /locus_tag="VMU21_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HUO77312.1"
                     /translation="MDITPEFIHKTTWNKVLKYIAASDEVPPYILMDREVVREKVSLI
                     GKHIRNSKVFYAVKANPDREVLHLVNNFGIGFEIASEGELQMLASLSVEPERIITSNP
                     IKTLKFLEQAVSYGIKYYAYDSFGEVEKLSRYAPGCNVYVRLSVPNEGSEWPLSKKFG
                     VELDEAGELLLYAKKTGLNPVGIAFHVGSQCNNVYNWNSAIEKAKDLWEVAEQNGIRL
                     SMLNIGGGYPIHYTKNVVDIETIEKTIDRAIAKKFPKDVEIFIEPGRAVVGDAGIFVA
                     TVIGKARRGDENWLYIDVGVFNGLMESIGGIKYVYIVGSRNDPEKWTIAGPSCDSFDV
                     IDRGVLLPEPEIGNRILILSSGAYTVSYASEFNGFSIPKTILI"
     gene            complement(11491..12156)
                     /locus_tag="VMU21_07015"
     CDS             complement(11491..12156)
                     /locus_tag="VMU21_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77313.1"
                     /translation="MEDAKLIGLNLSVASPEALEILIEDTADHEVFREILASNAHRPE
                     ILKLLIDSPNVPTEIKEAAWKHLSLLSPLPARPRHGDESRKHGLLQKIQQLTVGERIA
                     LALRGGQEIRSILLKDSNKEVVLTVLKNPRITETEVELVAHSRNIPEEALRTIHKNRE
                     WMKNYAINLALVNNPKTPPGIGITLVSGLRLKDIAVLERNKNVPEAIRAIAKKVLQAR
                     KPH"
     gene            complement(12156..12887)
                     /locus_tag="VMU21_07020"
     CDS             complement(12156..12887)
                     /locus_tag="VMU21_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HUO77314.1"
                     /translation="MKERLDILLVEKGMVSSREKARALIMEGNVLVNGMLVTKAGSLV
                     NSDALIELKGEEVPYVSRGGLKLEAALHHFGINLTGLIAMDVGSSTGGFTDCMLQKGV
                     RKVYCIDVGYGQLAWSLRNDPRVVLLERTNIRYLEKERIHDMINVATIDVSFISLKNV
                     LPKVMDFVRKGGEILALVKPQFEVGKGEVGKGGIVREEEKRVAAVDSIREFAEGLGFR
                     TGGVFQSPVLGQKGNHEYFLHLRRP"
     gene            13089..13883
                     /locus_tag="VMU21_07025"
     CDS             13089..13883
                     /locus_tag="VMU21_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77315.1"
                     /translation="MKQLITIFLLVLPYVLSSAAFGQVSGKVLDIPTRPGVTQRFLYL
                     VPVNPKATAILFAGGHGGLQIRDDGSFGWGGGNFLVRTRQLFADNGLAVVVLDAPSDR
                     QSAPFLSGFRQTPEHVADVRAVIAWLKHRAGIPVWLIGTSRGTQSAAFVATQLPVRGS
                     GPDGLVLTSTILTDDKGRPVPEMALEKITIPVLVVHHEQDGCKHCAYSEIPRLMNKLS
                     ATSKKELAPFKGGKNRGDPCEAFAYHGFNGIEEDVIGKIVTWIKAR"
     gene            complement(14172..16061)
                     /gene="dxs"
                     /locus_tag="VMU21_07030"
     CDS             complement(14172..16061)
                     /gene="dxs"
                     /locus_tag="VMU21_07030"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HUO77316.1"
                     /translation="MLIRNIESPADLKKLAKEELPLLAEELRDIIIEQVSCEGGHLAS
                     NLGVVELTIALHYVFDTPTDKIVWDVGHQSYAHKLLTGRYKKFSTLRKYKGLSGFPKM
                     DESPFDAFGTGHSSTSISAAVGIAEARDKRGERFKVIPVIGDGAMTSGLAFEGLNNAG
                     QLKKDLLVILNDNEMSISKNVGALSAYLNRILTGEAFQRLKKETKSFLEHIPKLGAPV
                     SKMAQRAEETLKGLFLPGILFEELGFTYIGPIDGHDIDLLIETFNRIKQLNEPILLHV
                     ITKKGRGYKFSEKDPCVFHGIGPFDMDTGTITSDPTTPTYSEVFGKALTALAQEDIRV
                     VAISAAMREGTGLDIFSEKFPERFYDVGIAEPHAVTFAAGLASHGLRPVVAIYSTFLQ
                     RAYDEIIHDVCLQNLRVIFAIDRSGIVGEDGATHQGVFDISYLRHIPNLLVMAPKDAP
                     ELRGMLRLATRHDGPSAIRYPRGRVKQPLGYGVKETTVEIGRGELLSEGDDIAIIAIG
                     NTVSPSLRAAEMLRQEGISPSVINARFIKPLDEDLILSTASRIKRIVTVEENVLAGGF
                     GSAVLELLNKSGLNDVSIRRIGIEDEFVEHGSQSVLRKKYSLDEEGIYRVCKNHLASL
                     PLDKF"
     gene            complement(16092..16982)
                     /locus_tag="VMU21_07035"
     CDS             complement(16092..16982)
                     /locus_tag="VMU21_07035"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HUO77317.1"
                     /translation="MDLKVYLKEKRGMVDSFLQSYFESCVSPALLNDAMAYSVFAGGK
                     RVRPILALASYEACGGKAEDIVPFASALEFIHTYSLIHDDLPAMDNDDLRRGKPTNHK
                     VFGEGMAILAGDALLTEAFYLISSALLNGTHIRPRSLLKLIREVAMAAGAQGMVGGQA
                     EDILSEDAAMESDNLSFIHTHKTGALITASVRMGGILANAGRRELSALTRYGENIGHA
                     FQIIDDILDVRGESAELGKSTGSDERKKKMTYPGLYGIDLSMNKAESLVESALDALQC
                     FSEKADPLREIALYILKRRN"
     gene            complement(17055..18593)
                     /gene="guaA"
                     /locus_tag="VMU21_07040"
     CDS             complement(17055..18593)
                     /gene="guaA"
                     /locus_tag="VMU21_07040"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HUO77318.1"
                     /translation="MMDHKILVLDFGSQYTQLIARRIRENRVYSEIYPFNASLDKIMS
                     FRPQGIFLSGGPSSVYDSGAPLLDARVFALGIPILGICYGMQLMAHALGGRVAKAQRR
                     EYGRAELVVDDDADLLARMPKDSVAWMSHGDRIERPPKGFVRIAHTDNSPIAAMADRK
                     RSFYALQFHPEVVHTVHGKKIIKNFLFKICGCKPTWTMKSFIETAKREIKTLTGERQV
                     VCGISGGVDSSVTAVLVHEAIGDALTCIFVDNGLLRAGEAKKVEHTLRKSFHMNIRCV
                     DSSSRFLTRLRGVTDPERKRKIIGNEFIRVFEEEAAKIKNAGFLAQGTLYPDVIESTS
                     FKGPSATIKSHHNVGGLLKKMKLKLVEPLRELFKDEVRLLGKELGMPDEIIHRHPFPG
                     PGLAIRCIGDITKERLEILRKADTIVLEEIKKAGLYGSLWQAFAVLLPVKTVGVMGDS
                     RTYDNVIAVRCVESVDGMTADWSKVPYNLLERVSGRIINEVKGVNRVVYDITSKPPGT
                     IEWE"
     gene            complement(18687..>19076)
                     /locus_tag="VMU21_07045"
     CDS             complement(18687..>19076)
                     /locus_tag="VMU21_07045"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HUO77319.1"
                     /translation="LGSLFAGTDESPGETVLYQGRSYKVYRGMGSLGAMEQGARDRYM
                     QEGIEAKKLVPEGVEGRVPNKGPLSQSVHQLIGGLRSGMGYCGCKNLLELRTKTKFIR
                     ITSAGLRESHVHDVIITKEAPNYRIEQ"
BASE COUNT         4296 a         4926 c         4574 g         5280 t
ORIGIN      
        1 aaagtttgcc cttgtggaga atttctggga gaccggcttt ggcatgccct ctttcacgct
       61 cctgggaccg aagatcatcc gtttcccctt catcatcaac tcctcttatc cccacgaaat
      121 actccataac tggtggggaa acagcgtctt tcctgactct gcaaaaggga actggtcaga
      181 aggattgacc gcatacctct ctgaccatct cataaaagag cagcagggga gcggcgtgga
      241 gtaccgcgaa acgaccttgc agaagtatgc ggactatgtg gtggctggaa gggattttcc
      301 ccttgttgat tttcgttccc gccacagctc tccttcagag gcggtgggat acggcaagtc
      361 ccttatgttt tttcatatgc tccgaaggga attgggagat aggagtttca tcgcggggct
      421 tcaggatttc tacaacaaaa acaaattcag ttttgcatct ttcgatgatt tgagaacaag
      481 ctttgaaaag gcatcgggca agaacctgaa gggtgagttt gagcagtgga tagagcggcc
      541 cggcgccccg gagatacgat taagtgatgc caaaagcagg cccgaaggtg aaggatatct
      601 cctcaccgct gttcttgagc agattcagga agggaagggg tatcgcttag ccgtacctat
      661 tgtcattacc atggaggctc aggagaaggc ctatgagact gtggtgaata tggagaacag
      721 gcagcttgat cttaaaatct ctttgccttc ccgtcctttg aggattgatg tggatcccca
      781 gtttgatctt ttccgcaggc tgaaacgaga cgaggtgcct cccgccatat cacaggcatt
      841 ggggacgaaa aggatggtgg taatccttcc ctcttcagaa aaaggccact tgctgagtgc
      901 atatcggaaa ctagccaaaa ccctgggaag ttcaggacct gatgaggttg aggtgaagct
      961 cgacacggaa atggaaaaac tccccccaga cagcgcagtc accatcatgg gatgggagaa
     1021 ccgctttctc aactccattg tatcagccct ttctccccat gaagtggcca taaatcaaaa
     1081 aggcatacgt atcgaaaaaa ctgaaatacc gcgtgaaaat cactccgtgg tgctgactgt
     1141 aagaaaccct caaaataagg acctgtgctg gatgttcatt gcttccgaca aagctgaagc
     1201 cctcagaggg ctcggtcgta agcttcccca ttatcacaaa tacagctatc tgggctttga
     1261 aggcgatgag ccggaaaata ttgcaaaagg ccgatggcct atacttgatt cccccatgac
     1321 agcaattttc tccagcaaag acggtaagct tgtaaaggtc gggatgggaa tgctggcgcc
     1381 acggaagcct ttggcagagc agtaggcaca gatatggcgg aaaggtgaaa tggatgagtg
     1441 aaagcgaagc ggattctcag gatgttattt tttggagtgg ctctgtttag gttttttcat
     1501 cttctttttt tgtggtaatt ggccgggatc gataattcct tcttctgcga gcactttctt
     1561 gtgaaagtcg cactcaaaac aaagatgact ttggccggta caggggtctt taaggttata
     1621 gagggaatat gaacccacaa tggcccagca tatccttccc ccattgattc cctcgttgat
     1681 tccgtctgca tagggatgaa cggctacagg gcataccccc agttccttga cttttttgcc
     1741 attcagctct cttccgcatt tcatatattc ccagcaattc agcttccgtg gcatgagagt
     1801 tcgttacctt ctttttcgtt ttggcgagaa cctttcctga aaatccccta gttaagactt
     1861 ttaacacggc gaggacagac gggtcaaatt aataaatcaa atcaaagtat atgaatttct
     1921 taagaaagga tgaagtatat gcaaacctgt gtgttaccgc gatttcattc ttggtgtgtg
     1981 aactcgtaaa atagagaagt gaatatgaag acattcatcc atcttgacgg tgcactggga
     2041 gatgtgttct tgtcccttcc ctgtatctgc gtcataaggg ttaattcgga ttttgtccat
     2101 atggccggac cacccgatgt tgcggactta ttgaaggagg tcgggtatgc tgatgaaata
     2161 accccttcag ggagcgccct ctttacatcc ctctatgcat tggagatgga gcagggagcg
     2221 atgggctttc ttgagcagtt tgaccatgca gttgtcttta caaggcgaag ggattctccc
     2281 ttggccttga atattgaaaa ggtgatccct cacaccgaga tcattattac cattcctcct
     2341 gagggagtga aaactcacgt ggcacaattc agactgaacc agcttgccct tggagtagaa
     2401 tctgttgacc ttagatcgaa gcttaccatt ccttccccgt atcgcgaaaa agcaaaggaa
     2461 ttccttgttc gttatttcgg tacagaggga agggggcctc tgatagcatt ccatcccgga
     2521 agcggaggca agagaaagtg ctggcccctg aacaactatt tcaggctggt ggaaaagttg
     2581 ctgaatcacc tggcctgccg gattcttttc ctcacaggtc ctgccgaaga acctgaagtg
     2641 gcagatgaaa tccttagctt tgcacacgga cataaagggg ttctccacgt gagaaacgag
     2701 ccactcatta tggttggctg cctcttggca aatagtgatc tctatgtggg aaatgattca
     2761 gggattacgc atctcgctgc tgcagtgaac ggaagggtgg tggtcctctt cgggccttcg
     2821 gatcctcttc tctggaggcc gttcggcaca ggagttcagg tgatctccgc gggaatttct
     2881 gaggattccc tggaaagagt gggcgtggac gaagtatatg caaggctgac ttcttctctc
     2941 agggaggcga caagggaata ctcctgatga gggtgaagct gatttgtgct ggttcgggaa
     3001 gagattatct cttcaacgcg acccgtgctt ttttcagcat ggaatggggc tgataagatg
     3061 atttggccag tctaagggta tccctgtcaa ggtcctgttc aaaattcagg aatctgtatt
     3121 ttgggggcag aatcctggca aaggtattga acatgtattg atagatgccc ttgaacctct
     3181 ttcgcgcctt ggcaaaatgg agtgcaaagg tctcttcatt caactcttca cccacaataa
     3241 atcccgcagg ttcgttatca gcataataga ttcctccgca caggatgagg tcatcgtatt
     3301 tcctgagagc ctcaaggcat ggggagtagt cggtctgggt ttcatcctgt tctgagtctt
     3361 ccagccatcc gttcagtacg gagagcgcat cgttcaggcg gtcattggtc agcggcaatg
     3421 ccacgtgggc ataggtggaa atgaattgtt tgaggagatt tctttttgag tggaggttcc
     3481 tccccctgta cgtgctcatc tgctccactg tgtagaggta atccatatcc ccttgtttgc
     3541 aggtgcactg atactcatcc tggctaaaac atttcagcca ggtctccgga atcggaaaga
     3601 ggaaatccac gctgctcatc attgcattta tatagccaca ttccattttt tcaatctcca
     3661 ccgtgggcat caaaaacgta tatccgtcat aggactgacc ctttatgaaa attcccttat
     3721 caaggatcac ctcgtacctg tgagcatccc tgaagaggta gaggtttggg aagctgtact
     3781 cagctatggg gcattggagt ttcctcagtc tttcatggag cagttcccta tggctgaaat
     3841 caagaggctc aacgtgcatg gtgaagtccg ctctacacta gatccctgtg tcgagatctc
     3901 cccacacata ctgaaccagt gcggtcgctg caatggcagc agtttctgcc ctgaggatac
     3961 ggttccccat ggatgtgacg agaaaacccc ttgatcgggc catgtatgcc tcctcttttg
     4021 taaagccgcc ctcaggtcct atggagatca tcaatgacct gcatcctccc agcacgtccc
     4081 tggccttggt gagcttcatc cctccctctt cccagaagag gagacctctt gagtttctaa
     4141 agtactgtgc atacagagac gaaggggcaa agagctctcg aaaggggagc agatcgtgga
     4201 ttctgggcac atgggtcctg ccgcattggc gtgaggcatc ttctgctatt ttcttccatc
     4261 tgggtccttt ccttgtctcc ctcagctggg accgttcagt ggtcaccggg agtatttcgt
     4321 tgacgcccag ttccgtagtc ttttggatga ggaactccat cttctcaccc ttgagtactc
     4381 cctggataag gatgatattt acctttgatt ccgtgtcata gggaacagcc cccttgaccc
     4441 gggcgaaaag ctctctattt ttgacggagg tgatttcggc attgtaagag gaaccctttc
     4501 cgtccagcac catgatctca tcccccacat gggaccttaa cacattgatg agataccggg
     4561 ctttttcacc tgcaatatga atggtttcag gccgttcttc taatggcagg aatattcgcg
     4621 gcatggagct atttttcagc gcctgaaaaa atgtccttga gcttgcttgt gaatgtcttt
     4681 gatgtcttgt ccccatctat ttgcgcaaat tcctcgagga gttcccgctg acggggagta
     4741 atatgcttcg gaatctcaat atttaccact atgatctcat ccccttttcc atgcccgcca
     4801 agtctgggga tacctttgcc cttgaggggg aacgccttcc ccggaggggt gcctgggggg
     4861 accttcagat gcgcgctacc ttcaagggtg ggaacctcta tctctgctcc caagacagcc
     4921 tgggggaagg tgatggtcgt ctcatggtaa agggtattcc cttttctcac aaagagagga
     4981 tgttcctcaa tgtcgatgat aatgtagagg tcccctggcg gaccaccgta tatacccggt
     5041 tcgccttccc ctgacatcct caggcgcgat ccggaatcca ctcctgccgg gattcttact
     5101 gatatggtgt ggaccttctt cactttccca tggcctttgc atgaagggca ggggtcggtg
     5161 atgatctttc ccgttccatg gcaggagccg catgtcttgg aaacgctaaa aaagccctgc
     5221 tgaaacctca catggccggt gcctttgcaa ttggtgcagg tgacaggacc tttgccgggc
     5281 tttgaaccag aaccgctgca tgaggagcat gtctcccacc gtggcacctc aatggtcttt
     5341 tcggtgccaa aagcggaatc ttcgagggtg atatcgaggt catacctgag atcattcccc
     5401 tttgctgccc tcggccgcct ctggctgcta aaaccgaaga tgtccccgaa aatatcgtca
     5461 aagatatccc cgaagcctgc tcctgtaaag gcgccaaatc cggcgcccat gtcctctgcc
     5521 gacccgtatc tgtcgtagtg ggccctcttt tcaggatcgc tcaggacaga gtacgcttca
     5581 tttatcacct tgaacttctc ttcggcatcc cggttaccct gatttctgtc agggtggtgc
     5641 ttgagtgcga gtcccctgaa cgctttcttt atctctgctt cggtggcatt tctcgggacg
     5701 ccaaggatct catagtaatc tttcatttct ccttcttatc aacgtcctcg aattctgcct
     5761 cgaccacttc ctcttcaggt tttgcctttg tttccggtcc tgaggtctgc gcacctgcgc
     5821 ctgcacccgc agccttgtaa atgtgttccg cgatcttata ggatgccttt gtaaggtttt
     5881 ctactgcgct ctttatctcc gcggcatccg tacttgtctc tttcaccttc ctgcattttt
     5941 ccagggcctc ttcaatctcc ctcttctcgg tttcggtcac cttgtctccg tactccttaa
     6001 gggacttctc cacagtgtat acgagagtgt cggcctcatt ttttgcctct gccaactgtt
     6061 tcttcttctt gtcctcatct gcatgggatt ctgcgtcacg cgtcatcttt ttgatctcgt
     6121 cctctgaaag gccgctggaa gcggttatcc ttatggactg ctccttgccc gtgcccatgt
     6181 cctttgcaga gacatggagg attccgttgg catcaatgtc aaaggagacc tctacctgcg
     6241 gcacgcctct gggtgcaggc ggaatcccta caagttcaaa taccccaagc agcttgttat
     6301 ctgcagccat ctccctctca ccctggtgga tcttgatggt aaccgcgggt tggttatccg
     6361 aggcggtgga gaatatctgg ctcttctttg tgggaatggt ggtgttcctc tcgataatct
     6421 ttgtgaagat accgccgagg gtctcgatac ccagggagag gggagtcaca tccaggagca
     6481 ggacctcttt gacctcgccc ttgagcacag caccctggat tgcagcccct accgccacca
     6541 cctcatccgg attcaccgtc cggttcggtt ccttcccgta gaatgactgc accacctgct
     6601 ggaccttcgg cgtacgggtc tggcctccca cgagtattac ctcatcgatg tctttggtgg
     6661 aaaggcctgc atcagcaagg gctaatttgc aaggttcaat ggtcttctca ataaggtctc
     6721 cggtgagttg ttccagcttt gccctggaga gcttcatgag cagatgcttt gggcccgttg
     6781 catccgcggt aatgaacggc aggtttattt ctgtctcggt ggcggtggaa agttcaattt
     6841 tcgctctttc cgctgcctcc tttaatctct ggagcgccat tctgtcctgc ttcaggtcta
     6901 tgccctgctc ttttctgaac tcctccacca tccagtccat gaccctgaga tcaaagtcgt
     6961 caccaccaag gtaagtgtcg ccgttggtgg acttaacttc tatcacccct tcccctatct
     7021 cgaggacaga gatatcgaag gtccctcctc ccagatcata tacggaaatc ttttcctcct
     7081 tcttcttgtc aagaccatag gcaagggatg ccgcggtagg ctcattgatg attctgagga
     7141 cattgaggcc ggcaattttc cctgcgtcct ttgtcgcctg tctctggctg tcatcaaaat
     7201 atgcgggaac cgtgatgacc gcatcggtga caggttcccc gagataatcc tctgctgcct
     7261 gtttcagctt ctggagtatc atcgcggata tctcgggagg agaatagttc tttcccctta
     7321 tctccacgtg cgcgtcccca ttagaagctt caacgatctt gtagggaaga cgctttttcg
     7381 catggtcaac ctgtggtgta ttgtattttc ttcccatcag ccgctttatg gagtatatgg
     7441 tattttccgg atttgtgatg gactgtctct tcgctatctg gcccaccagc cgttcccctt
     7501 tttcggtgaa cgcaaccaca gagggggttg tcctcgttcc ctcctggttg gggatcacca
     7561 ctggttcacc tccctgaaca actgcgacga ccgagtttgt ggtaccgagg tcgataccga
     7621 tcgcttttgc catgttattc ctcctttgtg ttttcgttta ttctaatttt cgtttccgtc
     7681 tcgtcaactg gtcttcttga aaccgaaacc agcgaaggtc tcaggacctt gccgctaaac
     7741 atatatcctt ttctgaactc ctcaaccaca gtcctatcct ccatgtcagt cctttccacc
     7801 tgggacatgg catgatgcac ggccgggtca aagggtttgc ccttggcagg tatggggata
     7861 aggccgaatt tctcggtaac cctctggaat tccctcaggg ttatctccac accctttaca
     7921 agaccctcgg acacgctatc cgcggaatgc ttcagcgcca tttccaggtt gtccattacc
     7981 gggagaagat gatacaggat gtcttcattg ctatacttta taagatcctc cttctcccgc
     8041 tggatccgct ttttgtaatt atcaaactcg gcatagagcc ggagatactt atcgttcaat
     8101 tccgtgagac tctcctgcaa tggctgcttc tctccccctt tttccgagtc cgtaggcagc
     8161 ggggtctctc cctcttgctt ctctgtcact tcttcgtttt ccaacatttc ctcctcattg
     8221 gcaccctctg cagactgtgg acaaagaata tgagaatggg tgattacctc tccgagagaa
     8281 tcttagtgat gaatttcgca gtagtatcca ctatagtgat caccttcata taatccatcc
     8341 tggtgggacc tataatccct atggtcccta cgggcctgtc accctccctg taagaggata
     8401 ggaccatgct caactttttc atctctgagg cagggttctc agagcctatc agtactctta
     8461 cgccctctga ctgagggagc atgtccagaa gctttataat gagatgcttg tcctcaatgg
     8521 ccttggatat ttcctttatt ctctggatgt ccgtaaaatc cgggagtccc aggacttcat
     8581 gaaaccctga gataaacacg tcaccggagt cgaaggagag cgcctcccgg catattctta
     8641 tggccctgga aataagggta tcacacagcg ccttatcctg gtgcatctct ctcaggacac
     8701 tggaccttat ttcatccagg gtctgtcccg caaattcgga attaaggtat cccgaaattc
     8761 tatccaggtc atgttgcgtc aggtctggat ccagctgtat taccttgttt tttacaattc
     8821 cctcatctgt tatgagcaca atcattacct ggcgctcctt atatttaaag aggttgattc
     8881 ccttgagtgt ggtgttgttg ggattggggg gcaacgctac tcccaggtaa tgggaaaagg
     8941 ttgagagggc cctggaggtc tcccttaaga gtgcattgat atcggtcctg atattctcca
     9001 gcctttttat tatttcatcc atgagagttg cgtccggagt accctctttt tgcgtaaggt
     9061 aatccacata aagccggtat gccttgtctg tgggaacacg gcctgccgag gtgtgtggct
     9121 ggtggaggaa gcccatatcc tcaaggtcgg ccattatatt tcttatggtg gcggaagaga
     9181 gattaaatgc atacttcttg gtgacaaaac gggagcccac aggatcagga tttataatat
     9241 agctttcgac cactgcccag agtaccttat gaatcctttc atcaaacatt ctttatattt
     9301 tccgccctta gcactctcaa gggaagagtg ctaatctaat ttaacaatta cctcatggct
     9361 gtgtcaagat gggttaaccc cttaccattg aagctttctt ttcatgagtt tttcgtacat
     9421 tttgtggggc aaaaagcact gctggtgtat ctcgtcgctg tagtactttg tatctatctt
     9481 gaactctctc ttcattttcc gggggtcatg cttttttgat gcaaccgaga aggtccacca
     9541 gtttccggcg taggtgacca ggggtgcggt ataaaggtcg acaacaggga agatcagccg
     9601 cattgcctct tgcacatcga tgaccatatc cttatgaaaa tggagggatt ccgagaggga
     9661 aacaaacatg ccttcatcgg taaggcagtc cttcacggat ttaaagaact ccactgtaaa
     9721 gaggctcttc gcaaaaccca caatatcggt agagtcgacg atgatcgcgt caatgtcgct
     9781 gttccttcct ttcacgaact ccgctccatc catgcagcgt atctccacgc gcgtatcgcc
     9841 cacatggctg gccactgtgg ggaaaaactt ccttgacaca ttgatgactt cctcgtctat
     9901 ctcgatgaaa taaacttttc tcaccgtatc gtgtttgagg atctccctca ctgccccacc
     9961 atcacctccg ccgatcacca ccacgttcct gggatgagga tgggagtgca acaccacatg
    10021 ggcgagcatc tcatgataaa agaattcatc cctctccgtg atctgtacca ccccgtctag
    10081 gatgagcatt tttccgaagt aggggttttc gatgaccaca atctcctgaa aagggctctt
    10141 gctcttatgg agtacctttt caacgccata gacatactgt atcggtgcgt agggatcttt
    10201 ctcaaaaaat ttgatcatgg aaacctccat ataataaggt taaggtttag gtcgaggaca
    10261 agaagagaat cttctctggg tctcagtctt gcccttagcc agatttttta tataagaatc
    10321 gttttcggga tcgaaaagcc gttaaactca gatgcatagg aaacggtata ggccccgctg
    10381 gagagaataa gaatacggtt gccgatctca ggttcaggaa gcagcacacc acggtctatg
    10441 acatcaaagc tgtcgcagct gggtcctgca atggtccatt tctcaggatc atttctgctt
    10501 cccacgatat acacatactt gattcccccg atgctttcca tcaggccatt gaataccccc
    10561 acatcaatat aaagccagtt ttcgtctccc ctgcgagcct tgccaattac ggtggccaca
    10621 aagattcccg catcacccac tacagccctt cccggctcga tgaaaatctc cacgtccttg
    10681 gggaatttct ttgcgattgc cctgtcaatt gttttctcta tggtttcgat atcaaccaca
    10741 ttcttcgtat agtgaatggg atatcctccc ccgatattga gcatgctcaa ccgtatgccg
    10801 ttttgttctg ccacttccca tagatccttt gccttctcga tagcgctatt ccagttgtag
    10861 acgttgttac actgggaacc cacgtgaaag gcgattccca cagggtttag ccccgtcttt
    10921 tttgcataga ggagcaattc tcccgcttcg tccagttcca cgccgaattt cttgcttaaa
    10981 ggccattcgc ttccctcatt gggaacggaa agccttacat agacattaca tccaggagca
    11041 tagcgtgaaa gtttttccac ttcgccaaaa gagtcatacg catagtactt tatcccgtat
    11101 gagacagcct gttccagaaa tttcagggtc tttatgggat tactcgttat gatccgttcc
    11161 ggttcaacac tcagagaagc gagcatctgg agttcgcctt ctgatgcgat ttcaaaacct
    11221 ataccaaagt tgttgacgag atggaggact tccctgtccg ggtttgcctt cactgcataa
    11281 aatacctttg agttcctgat atgtttccct atcagggaga ccttttccct caccacttct
    11341 ctgtccatca atatatacgg gggtacttcg tcgctggcag cgatatactt caggaccttg
    11401 ttccaggtgg ttttatgaat aaattcggga gttatgtcca ttgaagcctc tcatcactag
    11461 tatttgtcca tcggtgaagt tttgtggttc tcagtggggt ttccttgcct ggagaacttt
    11521 ctttgctatt gcgcgtatgg cctcgggcac attcttgttt ctttcgagga ctgcaatgtc
    11581 cttgagccgc aggccgctca caagggtaat gccgattcct ggcggtgtct tggggttatt
    11641 gaccagtgcc aggttgatgg cgtagttctt catccactcc ctgtttttat ggatggtgcg
    11701 cagcgcttct tcgggaatat tgcgtgaatg ggcaaccagt tccacttcgg tctctgttat
    11761 ccttgggttt ttcagcaccg tgagcacgac ctccttattg gaatccttca gaaggatgga
    11821 acgtatctct tggccgcccc tgagtgcaag ggcgatccgt tcgccaactg taagctgctg
    11881 aatcttctgg agcagaccat gtttgcggct ttcgtcccca tgccttggcc ttgccggtaa
    11941 cggggaaaga aggctgaggt gcttccaggc cgcctctttt atctcggtgg gcacattggg
    12001 actgtctatg agcagtttga gaatttccgg tctgtgtgcg ttggatgcaa gaatttcacg
    12061 aaaaacctca tggtcggctg tatcctcaat cagaatttcc agggcctctg gagaggccac
    12121 cgaaagatta aggccgatta gtttggcatc ttccatcacg gcctccttaa atgcaagaaa
    12181 tattcgtgat tgcccttctg tcccagaaca ggtgattgaa agactccgcc agttctaaag
    12241 cccaatcctt ctgcgaattc ccttattgaa tccaccgcag ccactctttt ttcctcttcc
    12301 ctcacgatgc ctcccttgcc cacctcaccc ttgccgactt caaattgagg ttttaccagg
    12361 gccagtattt ccccgccttt ccttacgaag tccataacct tcgggagaac gtttttgaga
    12421 gagataaatg atacgtcaat agtcgcgacg tttatcatat catgaattct ctctttttcg
    12481 agataccgta tatttgttcg ctccagaagg accaccctgg ggtcattcct gagtgaccag
    12541 gccaattgac cgtaccctac atcaatacag tagaccttcc ttactccttt ctgcaacatg
    12601 cagtcagtga agcctcctgt ggacgacccc acatccatgg cgatgagccc agtgaggtta
    12661 attccaaaat gatggagggc agcctcaagt ttgagtccgc ccctgctcac atagggaacc
    12721 tcctcgccct tcagttcaat aagcgcatct gagttcacca gagaacctgc ttttgtcaca
    12781 agcataccgt tcaccaagac attcccttcc atgatcaatg ctctcgcttt ttcacggctc
    12841 gagaccattc ctttttccac aagcagaata tcaagccgtt ccttcatcgc agtgtataac
    12901 aaaaaacaga gatatgcaga taaataattg tatcaattat atcagacatc aagaccattt
    12961 gttcagttct tgaccgtgtc ttgcatgagg cggctgggat gttcagggtt gtggctttgg
    13021 caaaagggga gggggttcac gaggcaatat gttgcaccgt acggaaaatg atgttagact
    13081 gaaaaaatat gaaacagcta ataacgatat ttctgctcgt tttgccatat gtactcagtt
    13141 ctgcggcctt tgggcaggta tcaggaaagg tgcttgatat acctactcgc ccaggggtga
    13201 cgcagcgatt cctttatctt gtgcctgtaa acccgaaagc cactgccata ctcttcgcgg
    13261 gtgggcatgg tgggctccag atacgtgacg atggaagttt cggatggggg ggaggcaatt
    13321 ttcttgttcg cacacggcaa ctattcgctg acaacgggtt ggccgtggtt gttcttgatg
    13381 caccttctga tcgccagagc gcaccatttc tgagcggctt tcggcaaacc ccggaacacg
    13441 ttgccgatgt gagggccgtg attgcgtggc ttaaacaccg ggccgggatt ccggtatggc
    13501 ttatcggaac aagtcgggga actcaatcag ctgcattcgt tgccacgcag cttcctgttc
    13561 gtggtagcgg ccctgacggt ctggtcttaa catcaacgat attgactgat gacaaaggac
    13621 gccctgtacc ggaaatggca ctagaaaaaa tcaccattcc tgtcttggtg gtacaccacg
    13681 agcaagatgg ctgcaagcat tgcgcataca gcgaaattcc ccgtctaatg aataagttga
    13741 gcgcgacttc taagaaagag ctggcgccct ttaaaggcgg aaagaatcgc ggtgatccat
    13801 gtgaggcatt tgcgtatcat ggctttaatg gaatagaaga agatgtgatt ggaaaaattg
    13861 tcacgtggat taaggctcgc taaccggcca aatttcttat atgtcctgcc gtattgtgcg
    13921 gaaatttatt ctgcggatta gcgcacaaag gtcagtttcg caagagatac aaattgcttc
    13981 gtaaacagtt atctattgcc gtcattggtt ggatgatctt gaggtgtgtg tggactgtct
    14041 gtgccatgtt ggtgctgtgg tgaagcccca cgggcccccc atatgctgaa ggtgggctac
    14101 gtggcgcgca gggatctcat gacggagcag ggcggcacag agcagcatca atatcatgag
    14161 aagaggtgat tctagaactt atcgaggggc agggaggcaa gatggttttt gcatactctg
    14221 taaataccct cttcatcaag ggagtatttt tttctcagga ctgactggga gccgtgctcc
    14281 acaaattcat cctcaatgcc tatccttctt atcgagacgt cattcaatcc tgacttgttc
    14341 agcaattcca gcactgcgga gccgaacccc cctgcaagca cattctcttc aacagtgaca
    14401 attctcttta tccgcgatgc agtggaaagg atgaggtctt cgtccagggg tttgatgaaa
    14461 cgggcgttaa tgacagatgg agaaatgccc tcttggcgta gcatctctgc ggctctcagg
    14521 gagggagaga cggtgttgcc gatcgcaata attgcgatat catccccctc agacaggagt
    14581 tctcctcttc caatttctac agttgtctct ttcacaccat atcctaaggg ctgctttacc
    14641 cttcccctgg gatagcgtat ggcagacgga ccgtcatgac gggttgcaag ccttaacatc
    14701 cctctcagtt ccggtgcatc cttgggagcc atcaccagga gattggggat atgcctcaaa
    14761 taagagatat caaaaacccc ctggtgtgtt gctccgtcct cacccacaat cccggaacga
    14821 tcaatggcaa aaattacacg gaggttctgc aggcacacat catggatgat ctcatcgtat
    14881 gccctctgga ggaatgtgga atagatggct acgacagggc gcaaaccatg actggcaagc
    14941 cctgctgcga aggtgactgc gtggggctct gcgatgccca cgtcataaaa cctttcagga
    15001 aacttttctg aaaagatatc aaggcctgtg ccttcgcgca tcgcagcaga aatggccaca
    15061 acccttatgt cctcttgcgc aagagcggtg agcgcttttc cgaagacttc gctgtacgtg
    15121 ggagtggtgg ggtccgaagt aatagtccct gtatccatgt caaaaggccc tatgccatga
    15181 aatacgcagg gatctttctc ggagaattta tagcctctgc ctttttttgt gatcacatgg
    15241 agcaaaatag gttcgttcag ctgcttaatt ctgttgaagg tctcgatgag aaggtcaatg
    15301 tcatggccgt caatgggtcc tatataggtg aagcccagtt cttcaaaaag gatgcctgga
    15361 aggaaaagtc ccttcagggt ctcttctgct cgctgtgcca tcttcgatac cggggctccc
    15421 aacttcggta tatgttcgag aaagcttttt gtctccttct ttaatctctg gaatgcctca
    15481 cccgtgagaa tccggttaag atatgcagag agagcgccca cattttttga tatggacatc
    15541 tcattgtcgt tcagtatcac gagaaggtcc ttcttgagtt ggcccgcgtt attcaaccct
    15601 tcgaaggcaa gccctgaggt catggctccg tcaccaatca cagggatgac cttgaagcgt
    15661 tcacccctct tatccctggc ttcggctatc ccaacggcag cagaaatgga cgtggagcta
    15721 tggcctgtac cgaatgcatc aaacgggctc tcgtccattt tgggaaagcc ggaaagtcct
    15781 ttgtattttc gtaatgtcga gaatttcttg taacgtccag taagaagttt gtgagcatat
    15841 gattgatgac ctacgtccca gacgatttta tcagtgggcg tgtcgaacac atagtggagc
    15901 gcgatggtta attccaccac accgaggttg gatgcaaggt gtccgccctc gcacgatacc
    15961 tgttcaataa tgatatccct aagctcctct gccagcaagg ggagttcttc tttggccagt
    16021 ttcttaaggt cagccggaga ttctatattt cttatcaaca tgtgcagctc cctcctcttg
    16081 atagaccctg ttcagtttct cctcttaagg atataaaggg cgatttccct taacgggtcg
    16141 gccttttcgg aaaagcattg caaggcatca agggcactct ccacgagcga ctcggccttg
    16201 ttcatcgaaa gatcgatgcc gtaaaggccc gggtaggtca ttttcttttt ccgctcatca
    16261 gagccggtgg atttgccaag ttccgcgctt tcccctctca catcaaggat atcatcgata
    16321 atctgaaagg cgtgaccgat gttctcacca tagcgcgtaa gggcggacaa ttccctcctg
    16381 cccgcattgg cgaggatacc acccattctc acggacgcag tgatgagggc gcctgtctta
    16441 tgggtatgaa tgaaagagag gttgtcgctt tccatggctg catcttcaga gagaatatcc
    16501 tctgcctggc ctcccaccat tccttgggca cctgcagcca tggcgacctc acggatgagc
    16561 ttcaggaggg aacgaggcct tatatgcgtc ccattaagga gggcgctgga aattaagtaa
    16621 aaggcctctg tcagaagggc atctcctgcg aggattgcca ttccctcgcc aaataccttg
    16681 tggtttgtag gtttgccccg tctgaggtca tcattatcca tggcaggaag gtcatcatgt
    16741 atgagggaat aggtgtggat aaactcaagg gccgaagcaa agggaacgat atcttccgcc
    16801 ttacctccgc acgcctcata agaggcgagg gcgagaatgg gcctgaccct tttgcctcct
    16861 gcaaagactg aatacgccat ggcatcgtta aggagcgcag gtgaaacgca cgattcaaaa
    16921 tatgattgca gaaacgaatc aaccattccc cgtttttctt taagatacac ttttaggtcc
    16981 atgttttttc tagctttttg cgcatcgcct ataccttatg gatacgtcta tgagccacga
    17041 gtcccagagc attgtcattc ccactcaata gtgcccggtg gttttgaggt gatgtcgtat
    17101 accaccctgt tcactccttt cacctcattg ataattcttc cggaaaccct ttccagaagg
    17161 ttatagggaa ccttgctcca gtcagcggtc atgccgtcca cactttcgac gcatctcact
    17221 gcgatcacat tgtcatacgt cctgctatca cccatgactc ccacggtctt taccggtaag
    17281 agtacagcaa aggcctgcca cagggagccg tagaggcccg ccttttttat ctcctccagt
    17341 acaatggtgt ctgcctttct gagaatctcc aacctttcct ttgtgatgtc accgatgcac
    17401 cggattgcca ggcccggacc ggggaaggga tggcggtgaa tgatctcgtc cggcatgccc
    17461 agctcctttc ccagcaggcg gacttcgtcc ttgaagagtt ccctcaaggg ctcaacgagc
    17521 ttgagtttca ttttttttag gagtccaccc acattatggt ggctcttgat ggttgcggat
    17581 gggcccttaa acgaggtgct ctctattacg tcagggtata atgtcccctg ggcgaggaac
    17641 cccgcgttct tgatttttgc tgcctcctcc tcaaagaccc ttatgaattc gtttcctatg
    17701 atcttccttt ttctctcagg gtctgttact cccctcaacc tggtgagaaa tctcgatgag
    17761 gaatccacgc accggatgtt catatggaaa gatttcctga gggtatgctc caccttcttc
    17821 gcctcgcccg ccctcaataa tccgttgtcc acaaagatgc aggtgagggc atcaccgatt
    17881 gcttcatgca caagcactgc agtgacggac gaatccaccc ctccgcttat accgcatacc
    17941 acctgtcttt cccctgtcag tgtcttaatc tctctctttg cggtctcaat aaaggatttc
    18001 atggtccatg tgggcttgca gccacatatc ttgaagagaa aattcttgat tattttcttg
    18061 ccatgcactg tatggaccac ttcaggatga aactgaaggg cgtagaaact cctcttcctg
    18121 tcggccatag cagcgatggg cgagttgtct gtatgggcga tcctgacaaa acccttgggg
    18181 ggcctttcta tcctgtcccc atggctcatc caggcaacgc tatcctttgg cattcttgca
    18241 aggaggtctg cgtcgtcgtc aacgacgagt tctgccctgc cgtattccct cctctgcgcc
    18301 tttgctaccc ttcctcctag cgcatgggcc atgagctgca ttccgtagca gatgccaagt
    18361 atgggaatgc cgagtgcgaa tacccgtgcg tccaggaggg gtgctccgga atcatacacg
    18421 ctagaaggtc cccctgaaag gaaaataccc tggggacgaa aggacatgat tttatccagg
    18481 gatgcattga agggatagat ttcggaatat accctgtttt cccttatcct tctggcgata
    18541 agctgggtat attgtgatcc gaaatcaagg actagtattt tatgatccat catgctctcg
    18601 gaatcggata gggtcaatcg gggatcaagg acacatggag agaaccctgt aagcaggtct
    18661 cagtattctt aactcctcgc ttcccgttat tgctctatcc ggtaattcgg tgcctccttt
    18721 gtgatgatca catcatgcac atgactctct ctgagacctg cgctggtgat tctgatgaac
    18781 ttggtttttg ttcttagttc caagagattt ttgcaaccgc aatatcccat tccggatcta
    18841 agcccgccga ttaactggtg tacgctttgg gaaagaggcc ctttattagg aactcttccc
    18901 tcaacgccct cgggaaccag cttctttgcc tcaatgcctt cctgcatgta gcggtctctt
    18961 gctccctgct ccattgcccc cagagaaccc atgcccctgt ataccttgta acttctcccc
    19021 tggtagagca ccgtctcccc aggcgattcg tcagttcccg caaagaggct tcctaa
//
LOCUS       DATKOU010000154         5961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1368923, whole genome shotgun sequence.
ACCESSION   DATKOU010000154 DATKOU010000000
VERSION     DATKOU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368923"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..730)
                     /locus_tag="VMU21_07050"
     CDS             complement(236..730)
                     /locus_tag="VMU21_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO77320.1"
                     /translation="MILKPDNRIIKTDKLGFTFEVGPCSTEDFSALVEMYSIFSPKPA
                     SQGLPPKDPEVCYNWVKRLFSIGMNFLAWRNDRVIGHAALVPHPNGKSGEFIIFVDQN
                     DRNLGVGTEVTRLTLEEARKLAFDSVWLAAENSNLIAIKLYRNFGFQFRDENIDERIM
                     LLKL"
     gene            1534..1704
                     /locus_tag="VMU21_07055"
     CDS             1534..1704
                     /locus_tag="VMU21_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77321.1"
                     /translation="MDLKLSGLEASAVLNALQVYMKELEKSGNEKGILIEKKTVKDLL
                     LRLEDMPSGEVP"
     gene            complement(1906..2223)
                     /locus_tag="VMU21_07060"
     CDS             complement(1906..2223)
                     /locus_tag="VMU21_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HUO77322.1"
                     /translation="MATATKVRERHLELDEDGFLVNIDDWDVHVVEQLAHKEGVKELT
                     GEHWKLIAALRLHYEKYGEAPLCRDILIEAGFSKMDMYKLFPQLGYRSAYKLAGLPKP
                     TEC"
     gene            2456..2710
                     /locus_tag="VMU21_07065"
     CDS             2456..2710
                     /locus_tag="VMU21_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77323.1"
                     /translation="MFKFIFRIIFIFIVLLIAFIALSVYSGGEKFRWFGKKVEQESQK
                     VGEKADQIKQGSGTVIKGIEKTTEKVKEFTGSKSKDEKSR"
     gene            2694..3374
                     /locus_tag="VMU21_07070"
     CDS             2694..3374
                     /locus_tag="VMU21_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO77324.1"
                     /translation="MKSLVEAQEIEKSFYTPAGELRILKGVNLSIGAGEIVGIVGASG
                     VGKSTFLHVLGTLDRPTLGKVFYDGKDIFSMDNAALALFRNRTIGFVFQFHHLLPEFT
                     ALENVMMPGLISFRDRAELKAHAEGILNELGVYERRDHRPGELSGGEQQRVAVARSLI
                     LNPRVVLADEPTGNLDTATGEELFHLLLRLNRERDITFVIVTHNESLSKRCHRVLTMV
                     DGRLENHS"
     gene            complement(3387..4058)
                     /locus_tag="VMU21_07075"
     CDS             complement(3387..4058)
                     /locus_tag="VMU21_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO77325.1"
                     /translation="MIEVTNLIKSYDINGQMLKAVDNVSFTVEKGGMVSIIGHSGSGK
                     TTLLSLIGGLTRPNSGEVIIEGQNIWSMSDDNLSEFRNKKISFIYQFSSLIPTLTSVE
                     NIMLPTAFGTFTEDSEGYARELLKVVGLEDKVSSYPSHLSGGQQRRVAIARAFINRPD
                     ILLADEPTGDLDEETEKEVIDLFHTMNQEKGVTIVIVTHNKDIALQAKKQFKMTNGIL
                     SVMGE"
     gene            complement(4081..4719)
                     /locus_tag="VMU21_07080"
     CDS             complement(4081..4719)
                     /locus_tag="VMU21_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77326.1"
                     /translation="MKKRVRAGINTMALMLLVALLLSPVACKKEEPQSERIPAQAYNP
                     YTTDSVLGEVKQKLEKNPDDVDALFHLADLYERNGQYPEAIETYKHLIKVKPGAGYAY
                     FKMGTAYDRINKPAEAIEAFKKSVQYMPKHAMTYNNMGVAYGKLNKYQDEIAALKKAI
                     QLRPSYSTARFNLGVTYLKIGNKKAAQQEYESLMKFDEGAAEALMKEIKKGS"
     gene            complement(4741..5139)
                     /locus_tag="VMU21_07085"
     CDS             complement(4741..5139)
                     /locus_tag="VMU21_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4418 family protein"
                     /protein_id="HUO77327.1"
                     /translation="MRHWKFGAILFFMGFLVLLTPRYILPTCEYQGLTRMACTYTGTA
                     EMFIGAIVMSAAVGIILSKDHGTLRWSCLTSLAAGISVLLVPEAIGYCHSSEHPCNYG
                     TVPVLRLIGVLIVLLSLTGFFASFKRVGKE"
     gene            complement(5136..5552)
                     /locus_tag="VMU21_07090"
     CDS             complement(5136..5552)
                     /locus_tag="VMU21_07090"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HUO77328.1"
                     /translation="MRRLISLSVLFALLAVSELLYAQPFPVPQGTMCSECGMMVNPDS
                     KFSVELVTTNGKKMFFCDIGDMLRHFRSDRGALKTIYVKDYTSGGWIDGEKALYVLNK
                     RFSTPMSWNIAAFSSESEAKKWGSPVGFSGAFGLVK"
     gene            complement(5560..>5961)
                     /locus_tag="VMU21_07095"
     CDS             complement(5560..>5961)
                     /locus_tag="VMU21_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUO77329.1"
                     /translation="ILLISAVLWVLALLMIGFAFYMIVNERQRELGLLRSMGAKKKHV
                     FRLIISEAMIISTVGGVIGLVAGSAILATFKDLILHSLKLPYLLPSVAMLIELVAGAI
                     IFSSLTGLLSSLVPAVLASRMEPYEAIRKGE"
BASE COUNT         1455 a         1427 c         1470 g         1609 t
ORIGIN      
        1 agaccttcca tgaagacctc aattacgttg agaagttgtg aggggggcgg gtaaatccgt
       61 cccagatgtt ggcgtgatgt acttcctgaa gtccgcaaca gttttggtta ttgatatccg
      121 attgttcttg ctccaactaa tcagatccag gaagaggcaa caggtgttta cttctcacca
      181 taatgcgcgt tctggatctt gtttttcttg gaataggctt tccgtgacaa atttatcata
      241 gtttgaggag catgatcctt tcatcgatat tctcatctct aaactgaaag ccgaaattgc
      301 gataaagttt tattgcgatt aagttggagt tctccgccgc cagccagacc gaatcaaagg
      361 cgagtttccg ggcctcttcg agggttaatc tcgtcacctc tgtgccgacg ccgagatttc
      421 tgtcgttttg gtcaacgaag attatgaact ctcccgactt cccgttcggg tgtggaacga
      481 gcgctgcatg gccgatcacc ctgtcatttc tccaagcaag gaaattcatc cctatagaga
      541 agaggcgttt cacccagttg taacagactt ccggatcttt tggggggagg ccctgagagg
      601 ctggtttggg cgaaaaaatg ctgtacatct ccacaagagc agaaaaatct tcggtactgc
      661 agggacccac ttcaaaagtg aatcccaatt tgtccgtttt gatgatgcgg ttatccggct
      721 taagaatcat aatcgccgta agcagtcaag tatgctaact ccctgctggc tgaaggcaga
      781 catcggcttt ttttcttgat tatctcgggt cagattatga agcgggtgtt agttctgagc
      841 caaaccgcga acgactccat aaacttgcgc aagaactcgc acgtattctc atttacgttc
      901 ttcattttgc tcccccttaa tctccgattg tttatcgcaa agggttcatc cctatcgcta
      961 ttatcagtat agcagagtcg acagatatga cataacagaa gctattcaat ctcggaactt
     1021 tcagaaatct cgcgttgatg gccacaatta ccggacgccc aggcaaggtt ctggacaatc
     1081 ttctggtacg tcgcgctgat cagccattga tggccgcagt atcgagggga ttgctcctaa
     1141 cgagaaaaat gtccgccgtc tccaccgcca aactttggct tgtgcgatgt tgtggctgta
     1201 cttcgcgccc tcaggttttc ccgaaggtgt tttattccag tcgtactctt gatagagttc
     1261 cggttttctg aaaatgtcgg tttgttgctg tgctccgtaa cccaacggcg accggttgcg
     1321 catttgttac agaaagggaa cgacaagact tttgttttct tgaaactaca gactcgtcgg
     1381 tgaagatacc aaaaaacaat tgcaggacgg tgcaatttcc cggcaggaag gcctattttt
     1441 atttagggaa gagcggatta ggccttgaca gtaaccacgc atatggtaat ctgtatatta
     1501 caactcaatt tagaaagaaa ggagaacagg acgatggatt tgaaattgag tgggttggag
     1561 gcatctgcgg tattgaatgc actccaggtg tacatgaagg agttggagaa gtctggtaac
     1621 gaaaagggaa tcctcattga gaaaaagacg gtgaaggatc tacttttgag gctcgaagac
     1681 atgccgtcgg gtgaagtccc ttagtccgcc cgcataaggg catggggcca ggcctgaata
     1741 tgccgctcgc tggctcttat gggctaagag tgatctctta agacagggct gttgcgtttt
     1801 tcgtgattgc cgcacttaga tcagtgtatc gaccccttta cgccctttcc agatttttgg
     1861 tggagggagt aaaggggtct ttccttttct aaccttgtag aagcattaac attcagtcgg
     1921 tttcggcaag ccggccagct tataggcact cctgtatcca agctggggaa atagcttgta
     1981 catatccatt tttgaaaacc ctgcttctat gagtatgtcg cgacacaggg gtgcttcacc
     2041 atatttctca taatggagcc tgagtgcagc gatgagcttc cagtgttcac ctgtcagctc
     2101 tttgactcct tccttgtgag ccagttgttc gactacatgc acgtcccagt catcaatatt
     2161 tacaagaaaa ccatcctcat ccaattccaa atgcctctcg cggacctttg ttgctgttgc
     2221 cataatgaca cctcctttat ttctacatta gccttcatga cagacgaata ctatgattta
     2281 tgtcataaat tcgggaaaat gaacagatac agtccctctg catctattta tggaagaggc
     2341 agatcaggga accctggatg cctcagggag agtttttctt tatattatcc attcttttca
     2401 cttttccttc ccttttctgg taaactcaat gaatatttgc gggaggagtg ggagcatgtt
     2461 caaatttatc ttcaggatca tttttatctt tattgttctg ttaatcgcct ttatcgctct
     2521 ttccgtgtat agcgggggtg agaagttcag gtggttcggg aaaaaagtgg aacaggagag
     2581 ccagaaggtg ggagaaaagg cggatcagat aaagcaggga agcggaactg taatcaaggg
     2641 aatagagaag accacggaga aggtgaagga attcaccggc agtaaaagca aggatgaaaa
     2701 gtctcgttga agcccaggag atagagaagt cattttatac tcccgcagga gagctcagga
     2761 tactcaaggg cgttaacctg tccatcgggg caggggagat tgtggggatt gtgggggcct
     2821 cgggcgtggg gaaaagcacg ttcctccatg tgctgggcac cctggacagg cccacattag
     2881 ggaaggtttt ttacgacggc aaggacatct tttctatgga caatgccgcc cttgccttat
     2941 tccgcaacag gactataggg tttgtgtttc agttccatca cctgctcccc gagttcactg
     3001 ctctggagaa tgtgatgatg cccggtctta tcagttttcg tgaccgtgca gaactgaagg
     3061 cccatgccga gggtattctc aacgagttgg gggtgtacga gagaagagac cacaggccgg
     3121 gggagctttc agggggagaa cagcagaggg tcgctgtagc gaggtccctg atattgaatc
     3181 ccagggttgt gctcgctgat gagcccaccg ggaacctgga cactgccaca ggggaggaac
     3241 tctttcacct tcttttgaga ctgaaccggg aacgggacat cacctttgta atcgttaccc
     3301 ataatgagtc tctttcgaaa aggtgccaca gggtcctcac gatggtggat ggaaggctcg
     3361 aaaatcacag ctgaccgctg atacccctat tcacccatca ccgataaaat accattggtc
     3421 attttgaact gtttttttgc ctggagggcg atatctttat tgtgggtgac gattacaatg
     3481 gtgacaccct tctcctggtt cattgtgtga aaaagatcga tcacctcttt ttctgtctct
     3541 tcatcaaggt cacctgtggg ctcgtctgca aggaggatat caggcctgtt tatgaacgcc
     3601 ctcgcgatgg cgaccctcct ctgctgccct ccggaaagat gtgagggata tgagcttacc
     3661 ttgtcttcca gtcccacgac cttgaggagt tccctggcat acccctcact atcctcggta
     3721 aaggttccga aggcggtggg gagcataatg ttttcgactg aggtgagggt ggggatcagg
     3781 ctcgaaaact ggtagatgaa gcttatcttc ttgtttctga actcagagag gttgtcgtcg
     3841 ctcatggacc agatgttctg cccttcgatg atgacctctc cggaattcgg tcttgtgagg
     3901 cctcctatga ggctcaaaag ggtggtcttt ccgctccctg aatggccgat gatggagacc
     3961 atccctcctt tctcgacggt aaaggagaca ttgtccactg ccttgagcat ctgcccattg
     4021 atgtcatacg atttgataag gtttgtgact tctatcattc cgcctccaat ggccgtattc
     4081 ctaggagcct ttcttgatct ctttcatcaa cgcctcggca gcgccctcat caaacttcat
     4141 cagagactca tattcctgct gcgcggcttt tttattcccg atcttcagat aggtcactcc
     4201 aaggttgaac ctggccgttg agtaggatgg tcttagctgt atcgcctttt tgagggcggc
     4261 aatttcatcc tgatacttgt tcagctttcc atatgcgaca cccatgttat tgtatgtcat
     4321 tgcatgcttt ggcatatact ggacggactt cttgaacgct tcaatagcct ccgcaggctt
     4381 attgatccgg tcataggcag tgcccatctt aaagtaagca tatcccgcgc cgggttttac
     4441 ctttataagg tgtttatacg tttcaatcgc ctcgggatac tgcccgtttc gctcgtacag
     4501 gtctgcgaga tgaaacaggg catccacatc atcaggattc ttctctaact tctgtttcac
     4561 ttcgcccaaa acagagtcag tggtataggg gttataggcc tgggcaggta tcctttcaga
     4621 ttggggctct tccttcttgc atgcaacagg cgaaagaagg agtgcgacca ggagcatcaa
     4681 ggccattgtg tttattcctg cacgaacacg ctttttcatc gttcactctc catggaaagt
     4741 ttactctttt ccgaccctct taaaagaggc gaaaaaacct gtcagtgaca gcaggacgat
     4801 caggacacca atgagtctca atacaggaac ggtcccgtaa ttacaggggt gttcgctgct
     4861 atggcagtag ccgatcgcct cgggcacaag aagcaccgat atgcctgctg ccaggctcgt
     4921 gagacatgac catctcaaag tcccatggtc cttggatagg attattccca cagcagcgct
     4981 catgacgatg gctcctataa acatttctgc tgtgccggta tatgtgcatg ccattctggt
     5041 caacccttga tactcgcacg tcggaaggat atagcgtggc gtcaggagta caagaaaccc
     5101 cataaaaaag aggatcgcac cgaatttcca gtgtctcatt tcaccaatcc gaatgcgccg
     5161 ctgaaaccaa cggggcttcc ccacttcttt gcttctgact cagagctgaa agcggcgatg
     5221 ttccacgaca ttggggttga aaatctcttg ttaaggacat ataatgcctt ttcgccgtca
     5281 atccagccgc cgctcgtata atccttgaca taaatggttt tcagcgctcc cctgtccgac
     5341 ctgaagtgac ggagcatgtc ccctatatca caaaaaaaca tcttcttacc gttggttgta
     5401 accagctcga ccgagaattt cgaatcgggg ttcaccatca tcccgcattc ggagcacatg
     5461 gtaccctggg gaacgggaaa tggctgcgcg tagagaagtt ctgatactgc cagtaatgca
     5521 aagagaacag ataaagaaat cagtctgcgc atctcccctt tactctcctt tccttatggc
     5581 ttcatagggt tccattctgc tggccaaaac cgcaggcacc agggacgaga gaaggccggt
     5641 gagggaggag aatatgatgg cgcctgccac caattctata agcatggcga cagaaggcag
     5701 gagataaggc agtttcaggc tgtggaggat gaggtctttg aatgtcgcga gaatcgcaga
     5761 accggcaacc agtcctatga cacctcctac agtggagata atcatggcct cactaataat
     5821 aagtctgaat acatgtttct tcttagctcc catggacctc aacagaccca gctccctctg
     5881 gcgttcattc acaatcatat agaacgcaaa tcctatcatt agaagggcca gcacccatag
     5941 caccgcactg atgagcagta t
//
LOCUS       DATKOU010000155         7198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_604355, whole genome shotgun sequence.
ACCESSION   DATKOU010000155 DATKOU010000000
VERSION     DATKOU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604355"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VMU21_07100"
     CDS             complement(<1..1502)
                     /locus_tag="VMU21_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUO77330.1"
                     /translation="MDKDAMGKNFNKDTIGITAVVLAIVILLSTREAYPAYREAELFD
                     LGYEQYLSSQSAKAIEAFTLFLGEFPRSSAKDAAMFWVGKSLIQMKRAQEARNIFDEM
                     AREFPESPLNPLAIREIEKLGEADVAKVSEPIVRGTDEGEERHMEALQRQEEWVAGKS
                     AESSAPPTGHTTYGVQVAALSSEEAANRLKKKLKQQGIEATIKKTSFREGTRFHVLTG
                     EFGTKREADLYAIKISKRTGLDAFSIKVERSEGGTSAPLSQKGPLGPNAERETSGQLL
                     IPHKNDSGKSETREPVMTKKDEMVAILIEGTQYTAQQVSTFMNNSFAAILKLGIKEVI
                     WRTGNVYEDFINEQVLYEEAKRAGVIHHDERGHEKTEEKYRLSPEEADYLQRYFAISD
                     LVDKKLKERPAERVVESLIVRYSDGDRREKELLASELQGHAKSGKPFGEIYALYPQRV
                     SYAVTRVETLQERIKNEIVSLEKGEISVFWSKEGYIILKPALQTLSFEPF"
     gene            complement(1457..2440)
                     /locus_tag="VMU21_07105"
     CDS             complement(1457..2440)
                     /locus_tag="VMU21_07105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUO77331.1"
                     /translation="MRICAERWCTRRLPVSPVLASLLMVVIPVVLCFPDNPHGAMPDR
                     VVAIVNNEAITLADYKLFMKYIGLQGKGDGVDEGLLQALIEEKIILYEAKRRGIEISD
                     GEVDAMVEKMGTENALSRDEMEKALEGEGMGVQGFKRLIKEKLMALKLVESEVDSRVR
                     VNEEEIEDFYQTHKRDYLKSPGKVEVTAIFLKLKEGATASEITDLKRKALKIMAQLSE
                     GESFEDLVDRYDDEYLKSTRGRFGEFEKGTLIPLLDKKIFSMNVGETSEPIWLREGVY
                     ILKVVNKTGDIYKPVGEVREQIRAYLSEEKREALLHAWIKTLWERTSIKIQ"
     gene            complement(2585..3340)
                     /locus_tag="VMU21_07110"
     CDS             complement(2585..3340)
                     /locus_tag="VMU21_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HUO77332.1"
                     /translation="MFIVKKIVTPFLLPPGLFIVLLLLSSLWLFQRKNREASAINFFL
                     ALLVWLFSISPVSDAMLRGLEGRFSIPGNPEGDVIILLGGGVNDVVPDLSGIGAPSEE
                     MMGRIITAVRLRRRLHIPIIVSGGIVFKDRKAEAPIAKRFLVDLGVPEHEIIIEENSR
                     DTNENARYAVRICDARGYSRALLVTSASHMKRAVLCFRNAGMEVTPFPSGFKSLGKKH
                     YGWEDFLPDPDELRKASTAMRECIGLLYYRFAY"
     gene            complement(3319..4032)
                     /locus_tag="VMU21_07115"
     CDS             complement(3319..4032)
                     /locus_tag="VMU21_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HUO77333.1"
                     /translation="MRTRNLWPRDIYAAREIQDILRKKVKILPLRRTPAYVAAVDAAF
                     SGNEVIAVSTLSAFPSLECRQDAVWREEIRFPYVPGLLTFREGHAMISALKRLRTPPD
                     VILVDGQGIAHPRGIGIASHIGVILGKPTIGCAKSRLIGEFREPQTRKGSWSHLYYKS
                     KEVGAVLRTRDHVKPLFISPGHMIDIPSSLEIVMQCLSGYRIPEPLRRADSLSRELSR
                     HGGHISETGCAKQCSLSKR"
     gene            complement(4034..5377)
                     /locus_tag="VMU21_07120"
     CDS             complement(4034..5377)
                     /locus_tag="VMU21_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUO77334.1"
                     /translation="MKKKKADSIVRAEYLLSMDDHLSVMIDGAVAVSDGKIACLGKYE
                     EIAAKYESGTVIGGSGRVIIPGLINTHTHAAMVYFRGLADDLPLKEWLEDHIWPAESR
                     WLSPEFVSDAVELACLEMLKAGITTYNDMYFFGDAAGSATKKMGMRAVLGAGIVDFPT
                     VAAKTVDEYIGKAGDFIERWKGDALITPCIAPHSLYACSTETLKKARKLADYHNVPVH
                     IHLSETQWEVQEIISKYGRRPVHYLKDIDFLEKRVLAAHCVWVEEDEIQLLARCGVGV
                     SHCVESNLKLASGIAPVIRMLDAGVKVTFGTDSAASNNDLNIFSELSTAAKLHKAVSG
                     DPTALDSRRALLMATRWGAEVLGLGELTGSIEEGKAADLVVVNLGKPHLTPIYDIYSH
                     IAYSVRASDVESVMVGGKVILKEGQFCYGDESGIMAKATQWAEKICKGRQKRDAQ"
     gene            complement(5460..6479)
                     /locus_tag="VMU21_07125"
     CDS             complement(5460..6479)
                     /locus_tag="VMU21_07125"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HUO77335.1"
                     /translation="MLKRKEKYVVSVVGATGAVGNEMVATLEERNFPAEKLRLFASER
                     SEGKTLQFEGSDIPVETLKEDSFRGIDIALFSAGAERSKIWAPIAAQSGCVVVDNSSQ
                     WRMDPTVPLVVPEVNSHDLKWHKGIIANPNCSTIQMVVVLKPIHDVARIRRVVVTTFQ
                     SVSGTGKKAMDELLQQTTDLLNFREVKCNVYPHQIAFNCLPHIDKFLENGYTKEEMKM
                     VNETKKIMGDDSIRLTATTVRVPVFRGHSESINIETEKKLSADEARGVLSAAAGIVVF
                     DAPEKNVYPLAIQVAGKDETYVGRIREDESIQNGLNLWIVADNLRKGAALNAVQIAEK
                     LLEMA"
     gene            complement(6507..7007)
                     /gene="leuD"
                     /locus_tag="VMU21_07130"
     CDS             complement(6507..7007)
                     /gene="leuD"
                     /locus_tag="VMU21_07130"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HUO77336.1"
                     /translation="MMILKGRAWKFGDDVDTDAIIPARYLNTSDPSELARHVMEDADK
                     DFPKKVQKGDVIVGGKNFGCGSSREHAPIAIKASGVNAVIAKSFARIFYRNAFNIGLP
                     IFESPEASGGIREGDSVEIDGDKGIIRNLTQGTEYTAKAIPQFMQELISAGGLIEWTK
                     KKLAKT"
BASE COUNT         1635 a         2051 c         1493 g         2019 t
ORIGIN      
        1 tcaaaaggct caaatgataa tgtctggagc gcaggtttta gaattatata cccttccttt
       61 gaccaaaaca cactaatttc ccctttctca agcgacacaa tctcgttctt gatcctttcc
      121 tgtaatgttt cgactctggt taccgcatag cttacccttt gcgggtatag tgcgtatatc
      181 tcgccgaagg gtttaccact ctttgcgtga ccctgtagtt ccgaggcaag cagttccttc
      241 tcgcgcctgt ctccgtcgct atatctcact atcagtgact caactactct ctctgcaggc
      301 ctctctttta atttcttatc cacaaggtcg gagatcgcaa aatatctctg gagatagtcg
      361 gcctcctccg gcgatagcct gtatttttct tccgtttttt catgtcccct ctcatcatga
      421 tgtatcaccc ccgccctttt tgcctcttca tagagaacct gctcattaat aaaatcttca
      481 tagacatttc cggttctcca tatgacttcc ttaatcccca gtttgagaat ggcagcgaag
      541 gaattattca taaatgtcga gacctgctgc gcagtgtact gtgtaccttc aatgaggatc
      601 gctaccattt catccttctt cgtcataacc ggctctcttg tttcgctttt cccggaatcg
      661 tttttatgag ggataagaag ttgccctgat gtctctctct ctgcattggg ccccagtgga
      721 cctttttgag ataagggtgc acttgttcct ccctctgatc gttccacttt aatagaaaac
      781 gcatccagcc cagtcctctt gcttattttg attgcataca ggtccgcctc tctttttgtg
      841 ccgaactctc ccgtcaagac gtgaaaacgc gtcccttcac ggaaactcgt cttcttaatg
      901 gtagcttcga ttccctgctg tttcagcttc tttttaagac ggtttgcagc ctcctcacta
      961 cttaatgcag caacctgaac accatacgta gtatgaccag tgggtggcgc ggaagactcc
     1021 gcagacttcc ctgccaccca ttcctcttgg cgctggagcg cttccatatg tctttcttct
     1081 ccttcgtcag ttcctctcac gatgggttcg cttactttcg ccacgtcggc ctccccaagt
     1141 ttttctatct ctcttattgc aagggggttc agcgggcttt ccggaaactc cctggccatc
     1201 tcatcaaaga tatttcttgc ctcttgagca cgcttcatct ggattaagga ctttcctacc
     1261 cagaacattg cagcatcctt tgccgaactc cgggggaatt cccccaagaa caaggtgaat
     1321 gcctcaatcg cctttgcaga ctgggaagaa agatactgtt catatcccag atcaaagagt
     1381 tcagcttccc gataggcagg atacgcctca cgagtactca gcagtatgac tattgcgagc
     1441 actactgccg tgatgcctat tgtatcttta ttgaagttct ttcccatagc gtctttatcc
     1501 atgcatgcaa caatgcctct cttttctcct cagagagata tgcacgaatc tgctccctga
     1561 cctctcccac gggtttgtaa atgtcgccgg ttttgtttac aaccttgagg atatacacgc
     1621 cttccctcaa ccatatgggt tcgcttgtct ctcccacatt catagaaaat atttttttgt
     1681 ccaatagagg gatgagtgtc cccttttcaa attcaccaaa tctccctcgg gtactcttca
     1741 ggtattcatc atcatatcga tccacaagat cctcaaaact ttctccctca ctgagttgtg
     1801 ccattatttt gagtgccttt ctcttcaggt ccgtgatctc actcgccgtt gctccttcct
     1861 tcagtttgag gaagatagcc gtgacctcca ccttgccggg gcttttcaga tagtctctct
     1921 tatgtgtttg gtaaaaatcc tctatctcct cctcgtttac tctcaccctg gaatccacct
     1981 cgctctccac gagtttcagc gccatcaatt tctcctttat gagtctctta aagccctgga
     2041 cacccattcc ctccccttca agcgcctttt ccatttcatc cctggataat gcattctccg
     2101 tccccatttt ttccaccatt gcatcaacct ctccgtcact tatctcaatt ccccttcttt
     2161 tcgcctcata gaggataatc ttctcctcta ttaatgcctg cagcagtccc tcatcgaccc
     2221 catctccttt accttgaagt ccaatatatt tcataaagag tttataatca gcgagcgtga
     2281 tagcctcatt gttcactatg gcaacaaccc tatcaggcat tgccccatgg ggattgtcag
     2341 ggaaacacag cacaaccggg atgaccacca tcaataaaga ggccaagaca ggtgagacag
     2401 gcagtcgtct cgtgcaccat ctctctgcac atattctcat gcatcaccct ctttgggaaa
     2461 aagcagtttg tcaaaccggt agctttgtat agggttaagt gtatcaaaaa aagcgggaaa
     2521 agtgagcagg ttgcgaagga caaccagtgc agccccctca cctccacgcc aaatgaccac
     2581 acatttagta agcgaatctg tagtacagca atccaatgca ctctctcatg gcagtagacg
     2641 cttttctgag ctcatccgga tcgggcagaa aatcctccca tccataatgt tttttcccca
     2701 aagacttaaa ccctgagggg aaaggtgtca cctccatgcc cgcattccgg aagcacagaa
     2761 ccgctcgttt catatggctg gcagaggtca caagaagggc cctggagtat ccccgtgcgt
     2821 cacatattct tactgcatat cgggcatttt cattagtgtc ccggctattt tcctctataa
     2881 taatttcgtg ttcagggacc ccaaggtcca cgagaaagcg tttggctatg ggtgcctctg
     2941 ctttcctgtc tttgaatact atgccccctg acacaataat gggtatatga agccttctcc
     3001 tgagtctcac agcggtaatg atcctcccca tcatctcttc cgaaggagcg ccgattcctg
     3061 agagatcggg taccacgtca tttacgccgc ctccgagaag tataatgaca tctccctcag
     3121 gattcccggg tatggagaat cgcccctcaa gccctctcag catagcatct gagacagggg
     3181 atatggaaaa aagccacaca agaagggcaa ggaaaaagtt aatcgctgaa gcctctctat
     3241 ttttcctctg aaacaaccag agagaagata acaacaacaa tacgataaag agccctggag
     3301 gcagaagaaa gggagttact atctttttga caatgaacat tgctttgcac atcctgtttc
     3361 ggagatatgt ccgccgtgac ggctgagctc tcttgaaagc gaatcagccc tcctgagggg
     3421 ttcgggtatc ctgtagccag agagacattg catgacgatt tccagcgagg aggggatgtc
     3481 aatcatatgt ccgggagaaa tgaaaagtgg cttcacgtga tccctggtcc tcaggactgc
     3541 ccccacctcc ttgcttttgt agtagaggtg tgaccagcta ccctttcgtg tctggggctc
     3601 cctgaactca cctataagcc ttgacttcgc gcacccgatg gtaggcttcc caagaataac
     3661 cccaatgtgg gatgcgatac cgattcccct tggatgtgca atgccctgtc cgtcaaccaa
     3721 tatcacatca ggaggtgtcc tgagcctctt gagcgcactg atcatggcat gtccttctct
     3781 gaaggtgaga agtcccggaa cataaggaaa ccgtatctct tctctccaga ctgcatcctg
     3841 tctgcattcc aatgagggaa aggcagagag agtggaaact gcaatgacct cattgcctga
     3901 gaaggccgca tcaactgcag cgacatatgc aggggttctt ctcaggggaa gaatcttcac
     3961 cttctttctc aggatatcct gaatctccct tgcagcgtag atgtcccttg gccagaggtt
     4021 cctggtgcgc atattattga gcatcccgct tttgtctgcc tttgcatatc ttttctgccc
     4081 attgtgtggc ctttgccatt attccagact catcaccgta gcagaattgt ccttccttca
     4141 gtatcacctt tcctcccacc attaccgact caacgtccga cgcccttacg gagtaagcga
     4201 tgtgggaata gatgtcatag atgggcgtga gatgaggctt cccgaggttc accactacaa
     4261 ggtccgcagc cttgccctcc tcgatgcttc ccgtgagctc cccgagaccg agaacctccg
     4321 cgccccacct cgttgccata aggagagccc tccttgaatc aagggcagtg ggatcacccg
     4381 aaacagcctt atgaagtttt gctgctgtac tcaattcact gaagatgttc agatcattat
     4441 tgctcgcggc gctgtctgtt ccaaaagtga ccttgactcc tgcgtcaagc attcttatca
     4501 cgggtgctat ccctgacgca agtttgaggt tactttccac gcagtgggag acgcccactc
     4561 cgcagcgcgc gagaagctgt atctcatcct cctcaaccca gacacaatgc gctgcgagca
     4621 ccctcttctc cagaaaatcg atgtccttga ggtagtgcac cggccttctt ccatacttcg
     4681 aaattatctc ctggacttcc cactgggtct ctgaaagatg aatgtgcaca ggcacattat
     4741 gataatccgc caatttgcgt gctttcttaa gggtctctgt gctgcatgca taaagggaat
     4801 ggggcgctat gcatggggta ataagggcat ctcccttcca gcgttcaata aaatctccgg
     4861 ccttcccgat gtactcatcc actgtcttgg ctgctacagt cggaaaatcc acaatccccg
     4921 caccgaggac cgctctcatt cccattttct tcgtagcgct ccccgcagcg tctccgaaga
     4981 agtacatatc attataggtg gtgattcctg ccttcagcat ctcgagacag gcaagttcaa
     5041 ccgcgtcaga gacaaactca gggcttagcc atcgcgactc tgcaggccag atatggtcct
     5101 caagccattc ctttaacggg agatcatctg caagacccct gaaatagacc atcgccgcat
     5161 gggtatgggt attgatcaga ccgggaatta ttaccctgcc acttccccct attacggtac
     5221 cggattcata ctttgcagca atctcttcgt atttccccag gcatgcaatt tttccgtcac
     5281 tgacggctac cgctccgtca atcatcaccg atagatggtc atccatcgag agtaggtatt
     5341 cggcacgtac aatgctgtca gccttctttt ttttcataga cacggaggga taatgagaag
     5401 gggggatagg aagggacatc atttctgtat ttccggccct ccggatttcc ccaccaatgt
     5461 tatgccatct ctaaaagctt ttctgctatc tggactgcat tgagggcagc acccttccta
     5521 aggttgtccg caacgatcca gagattgagg ccattctgta tggactcatc ctccctgatg
     5581 cggcccacat atgtttcatc ctttcccgcc acctgtatcg caagggggta gacatttttt
     5641 tcaggggcat caaaaacgac gattcccgcc gctgcggaaa gtactcctct ggcctcgtct
     5701 gcactcagtt tcttctctgt ctcgatatta atgctctctg aatggccgcg aaacacaggg
     5761 acccggaccg tggtggcagt cagccttata gagtcatcgc ccataatctt ctttgtttcg
     5821 ttcaccattt tcatctcttc tttcgtatac ccgttctcaa ggaatttatc gatatggggc
     5881 agacagttga atgcaatctg atggggatag acattgcatt tcacttccct gaaattgagg
     5941 agatccgttg tctgctggag gagttcatcc atggcctttt taccggtccc tgacacggac
     6001 tgaaacgtgg tgaccaccac tcttctgatt ctggccacat catggatcgg tttcagcacc
     6061 actaccatct ggattgtgga acagttggga tttgcgatga tgcccttatg ccatttcaga
     6121 tcatgggaat tgacctctgg aaccacgagg ggaaccgtgg ggtccatcct ccactggctt
     6181 gagttgtcca ccaccacgca tcccgattgc gcggcaatgg gcgcccatat cttcgatcgc
     6241 tccgcacctg ccgagaagag tgcaatatcg atgcccctga acgaatcctc tttcagggtc
     6301 tccacaggga tatcgctccc ctcaaattgg agcgtctttc cctcagatct ctcggaggcg
     6361 aagagtctca gcttctccgc cggaaaattc ctctcctcca aagtagcgac catttcgttt
     6421 cccacagccc ccgtggctcc cactaccgaa accacatact tttctttcct cttaagcatt
     6481 cccgttctcc ttatctctct tcattcttaa gttttagcga gctttttctt ggtccattca
     6541 ataagaccgc ctgcgctgat caattcctgc ataaactggg gaatcgcctt tgccgtgtac
     6601 tcagtgccct gtgtaaggtt cctgattatt cccttatccc cgtcaatctc cacactgtcc
     6661 ccttccctta tacctccgga tgcctcgggc gactcaaaaa tgggaagacc gatattaaag
     6721 gcattacggt aaaaaatcct tgcaaagctt tttgcaataa ccgcattcac ccctgatgct
     6781 ttaatggcaa ttggggcatg ttcccgtgac gaaccgcagc cgaaattctt cccccccaca
     6841 atcacatcac ctttctgcac ctttttggga aaatccttat cagcatcctc catcacatgc
     6901 cttgcgagtt ccgaagggtc tgatgtattc agatatcttg cgggaatgat ggcatctgta
     6961 tccacatcat cgccaaattt ccaggccctg cctttcagta tcatcatgcc tcccgtgctc
     7021 aagaaattat cctatagtat aaacagtcct ctgacccaaa atccacaggc atacgcctta
     7081 ttgtctttcc gcggggttgt tgagcatctc gtgatgaagc gaactgacag ggtgttagag
     7141 agagataatc tccgtttcca ttttctcgct ctcgagcaac gctatgctgg atttccct
//
LOCUS       DATKOU010000156         4280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2527749, whole genome shotgun sequence.
ACCESSION   DATKOU010000156 DATKOU010000000
VERSION     DATKOU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527749"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..930
                     /locus_tag="VMU21_07135"
     CDS             340..930
                     /locus_tag="VMU21_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUO77337.1"
                     /translation="MKGNMKRYIGTVVGKMLLITVVLSCLIVIPGFAMVFAARDSVKP
                     AQAAYEVQEMHPGIFRWMKNNSGMSEEVLSEVYRAAMKNINSDLILAICMVESNFNPT
                     ARSNKGAMGLMGIMPGAWLDELKTKGIVQDKGDLYQISNNIASGAYVLQKYLSSTRTL
                     ERALINYVGGDRDYVRKVMKALGELYLAKWSRPVVV"
     gene            complement(1147..1452)
                     /locus_tag="VMU21_07140"
     CDS             complement(1147..1452)
                     /locus_tag="VMU21_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77338.1"
                     /translation="MRKAMVLIFTVVLLSAVAKVSFSEMRGENMPPSHGMGAKNITPE
                     NFNEAKAGILQKIEERLNKLNEEKACVQAASNADELKKCRPQRPEGPGHKGMQKPSQ"
     gene            1704..2012
                     /locus_tag="VMU21_07145"
     CDS             1704..2012
                     /locus_tag="VMU21_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77339.1"
                     /translation="MDKKEEYIQKLDAQLRDWSAKIDELNDKAEKAKGDIKKEYTRQL
                     EALEALRESALKKLAEMTEASGQAWEELRIGAEKAWHELKTSLDNAMAKFKQPQPPVS
                     "
     gene            2141..2575
                     /locus_tag="VMU21_07150"
     CDS             2141..2575
                     /locus_tag="VMU21_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUO77340.1"
                     /translation="MQIQEPRLSRLGYVYVLLAAGLWALSGPAAKYLFQGGMSPFELV
                     QLRTTISAAMLFVWLVVWKRDLLSIARKDYLYFPLLGVTLATMQFTYLYSISRIQVAA
                     AVLLQYQAPAFIAAYAIFINRKRLSVGTLVSLFGAVTAVILW"
     gene            2569..3036
                     /locus_tag="VMU21_07155"
     CDS             2569..3036
                     /locus_tag="VMU21_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUO77341.1"
                     /translation="MVGAYNLNLLHMNRVGIISGLASAVAFALYSVRSEYGMRTYAPW
                     TVFFYALVFAAVIWNLLYPPFSAFHARYNASAWQWILFIGLFGTVLPFGLYNEGIKRI
                     RSARASITATLEPVMAGVIAYGFFGEMMEPWQIMGAGLVISSILLLQTTQDPA"
     gene            3224..4186
                     /locus_tag="VMU21_07160"
     CDS             3224..4186
                     /locus_tag="VMU21_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUO77342.1"
                     /translation="MMPEDLAELRKLYMGFTSARVVITANNLGFFDCLKKAANAADTA
                     KKLKTDERATEILLDALAGLGLVVKSKDGRYRNAVVSNRYLVKGMRLYQGDIVSHAST
                     MWQNFSCLDKVVRTGRPARQGFDHESFIMGMHNLTVLRTEGLIKAIGLSAVRTMLDLG
                     GGPGTNAMAMAKKGVKATIFDLPETIAIARKVARREGVKGLNYITGDFHADSIGSGYD
                     LILLSQIFHAFSIEENKALLSKCRNALNPGGRVVVQEFPINDARTFPPHSALFSVNML
                     VGTESGRCYPPREMKQWLAETGFRDIVVKNLPETVLVEGRGSRN"
BASE COUNT         1107 a          984 c         1133 g         1056 t
ORIGIN      
        1 tcctcgttat cataatcccg ctcctcttgc tctccatcta ccccacagcc ggccctcctt
       61 caaaaaactg ggggatggca agcatactca ggtagttgac tttttttctt cagataggtt
      121 aaaataaaaa aggtatacat aagaagttcc actcctggct gtcctgataa gcactcgacg
      181 cggcacgttc agcaactaaa gacaccaaca tgagttagga aataaagagc aacagcaggg
      241 attaacttct gctgcattac tgcagcttag gctgtttagg aaaaacgcgt gcagtaaaaa
      301 aaatgagcct aagcgcagag tatgaaggag gtgtgcaaga tgaaaggcaa tatgaagagg
      361 tatataggaa cagtagtggg gaagatgttg ttgattactg ttgtcctctc atgtctgata
      421 gtaatacccg gctttgccat ggtgttcgca gcgagggact cggtcaaacc tgcacaggct
      481 gcatatgagg tgcaggagat gcatccgggt atcttcaggt ggatgaagaa caactcaggg
      541 atgtccgaag aggttctctc agaggtatac cgtgctgcaa tgaagaatat caactccgat
      601 ctgatactgg ctatctgcat ggtagaatcc aatttcaacc ccaccgcgag gagtaacaag
      661 ggcgcaatgg gccttatggg catcatgccc ggggcctggc tggacgagct taagactaag
      721 ggaattgtgc aggacaaagg ggatctctat cagatatcga ataacatagc ctccggcgca
      781 tatgtcctgc agaaatatct ctccagcacc agaacgctcg agagggccct tattaattat
      841 gtgggaggag accgtgatta tgtgcgcaag gtgatgaagg ccctgggaga actgtatctt
      901 gcaaagtggt caagaccggt ggttgtatag tttccccggg gtgaccaggt tctgggctta
      961 agggatagaa aattctttgg tcgggaagtc ctcgaatctc ccggagttcc tcactttagc
     1021 gcttccggtt ttccgaactc ggcagatact atagcgcggg aatctaaaac ccctttcctt
     1081 tccccttaaa aaaagggatt agagaggggt tttaccacac ctttatcgtt cgccgatagg
     1141 gtgctgttac tgcgacggct tttgcatccc cttatgaccg ggtccctcag gcctttgagg
     1201 cctgcatttt ttgagctcat ctgcattgct cgcggcctgg acacaggctt tttcttcatt
     1261 gagtttgttc aggcgctcct caattttttg gaggataccg gcttttgcct cattgaagtt
     1321 ttcgggtgtt atgttctttg ctcccatacc gtgggaaggg ggcatgtttt ctccccgcat
     1381 ttcagaaaac gagacttttg ctactgcact gagcagcact acagtgaaaa tcaaaaccat
     1441 tgcctttctc atgtcttctc acctcctctc ttatgtgcgt gatcttgttt ataaccttag
     1501 acaaatggac acagaaaagg tttaaaggcg actaagaaaa agatctgcat gctcgtaaag
     1561 accgagaata gtaaataata aaacgtggct gaaactcaaa ataaaagacc gtgaattgtg
     1621 aaaggactcg gcaaaggctt gacagtcagg tattttaata tacactggat ttagaaagta
     1681 cgggagaggg gaaggaggga gccatggaca aaaaggaaga atatatccag aaactggatg
     1741 cgcagctcag ggactggagc gcaaagatcg atgaactgaa tgacaaggcc gaaaaggcaa
     1801 agggcgacat caaaaaggaa tacacgaggc agttagaggc actagaagca ttacgggaat
     1861 ccgctctgaa aaagcttgca gaaatgaccg aggcgagcgg gcaggcctgg gaagaactta
     1921 gaataggcgc tgaaaaagcc tggcatgagc tgaagacttc acttgacaat gcaatggcaa
     1981 aattcaagca gcctcaacct ccggtttcct gacatgccaa atggtgcaag ggtttcttaa
     2041 ttccaaagcg ccaaagcaaa agaccgtgag acatgaacag taaagtgcat aactaaagtc
     2101 cacctttcca agttcccgac cattgctgca taatacgggg atgcagattc aggaacctcg
     2161 actttccagg ctcggatatg tgtatgttct ccttgccgcg gggttgtggg ccttatcagg
     2221 ccctgccgcg aagtatctct ttcaaggtgg aatgtcaccc ttcgagcttg tgcagctccg
     2281 caccacaatc tctgcggcca tgctatttgt gtggctggtg gtatggaagc gggatctcct
     2341 cagtatcgca cgaaaggatt atctttattt tcccttgctc ggcgtcacgc ttgcaaccat
     2401 gcagtttacg tacctgtatt ccataagcag gattcaagtt gctgcggcag tgctgcttca
     2461 gtaccaggcg ccggcgttca tcgccgcgta tgcaatcttc atcaatagga aaaggctttc
     2521 cgttggtacc cttgtctcgc tttttggcgc agtcacggct gttatcttat ggtaggggcg
     2581 tataatctga acctcctcca tatgaacagg gtaggcatca tttcaggact ggcctcagcg
     2641 gttgcctttg ccctttattc tgtgaggagc gagtatggta tgcgtaccta tgcaccttgg
     2701 accgtgttct tttacgcact ggtttttgcg gcagttatct ggaatctgct gtatccaccc
     2761 ttttcagcgt tccacgcgcg ttacaatgcc tctgcatggc aatggattct gttcataggt
     2821 ctgttcggga cagtgctgcc ttttggtctt tataatgagg gaatcaagcg tatccgttca
     2881 gcgcgcgcaa gcatcactgc aaccttagag cctgtcatgg caggggtgat tgcgtatggg
     2941 ttttttggtg agatgatgga gccgtggcag atcatgggcg caggccttgt catctcttca
     3001 atactccttc ttcagacaac gcaggatccc gcgtaacaaa taaacgtgaa tcgtgagtgt
     3061 gaatagtaat tggagaatcg tgaaaggtgg gcagaagtac catggttgat aaagcaaaga
     3121 tccattggcg aaatagctct cacccatatc ccacactctc tgacacaggc ttcatgtctt
     3181 cttggtatag tagaagaaaa cattatctga aggaggtaat agtatgatgc ccgaagatct
     3241 ggcagaactg agaaaactct atatgggatt cacctcagcc cgcgtggtga ttaccgcaaa
     3301 taatctgggg ttttttgatt gcctgaagaa ggcggcgaat gcagccgata cggcaaaaaa
     3361 gctgaagacg gatgaacgcg ccacggaaat attgctcgat gcactggccg gcttaggact
     3421 ggttgtcaaa agtaaggatg gcaggtaccg gaacgcggta gtgagcaacc gctatcttgt
     3481 gaagggcatg cgcctctacc agggagacat tgtgagccac gcatccacca tgtggcagaa
     3541 cttttcctgt ctggataagg tggtgaggac cggaagaccc gccagacagg gctttgacca
     3601 cgaatcattc atcatgggca tgcataatct gaccgttctc cgcacagaag gccttatcaa
     3661 ggcaatcggg cttagtgcgg tcaggaccat gcttgacctt ggcggtggcc cgggtaccaa
     3721 tgccatggca atggcaaaaa aaggtgtgaa ggccaccatc ttcgaccttc ccgagaccat
     3781 cgcaatcgcc aggaaggttg cccgccgcga gggagtaaag ggccttaatt acattaccgg
     3841 tgactttcac gctgattcta tcggctcagg gtatgacctc attttgttga gccagatatt
     3901 ccatgccttt tctattgaag aaaacaaggc ccttcttagt aagtgcagga atgccctgaa
     3961 cccaggggga agggtggtgg tgcaggaatt ccccatcaac gatgcacgga cgttcccccc
     4021 ccacagcgcg ctcttctcgg tgaacatgct tgtgggtacc gagagcggcc ggtgttatcc
     4081 acccagagag atgaagcagt ggctggcaga gacggggttc agggatattg tggtaaagaa
     4141 tctgccggag acggtccttg tggaaggaag gggcagccgg aattaactaa cagaggggct
     4201 tcttaagtct tttagaggca gactcctctt ctttccgcgc tcgtccacac ggacaaaggt
     4261 tatggtggtt gagaaggcct
//
LOCUS       DATKOU010000157        11708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5605517, whole genome shotgun sequence.
ACCESSION   DATKOU010000157 DATKOU010000000
VERSION     DATKOU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5605517"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..625
                     /locus_tag="VMU21_07165"
     CDS             158..625
                     /locus_tag="VMU21_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77343.1"
                     /translation="MFVPLLTALHVTVVVLWIGGVAFVTIIIFPMLLKMEDSFEKVLL
                     FQRVENRFAKQARAYAWTAGITGAILLYLTGQHRALFTLNGLGATVMLMVWLVYTLVL
                     TFEKKIFALFFSQPEKLDAAKIFVRLNTFHWLVLGLSLAAVFIGVWTGHGGHF"
     gene            666..1088
                     /locus_tag="VMU21_07170"
     CDS             666..1088
                     /locus_tag="VMU21_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77344.1"
                     /translation="MQLRILFGVLFLLVSFISIGIAEENQESRNLIQREMIALDSALK
                     TTIDSLVLDEPGRIGPAFDEVDKIREQVEYAVEHKVRITLPRNQKRFKEFVRLDNKFH
                     HELQTLLKAAKKNKMGVVQKQTHRLLDACVRCHAIFRK"
     gene            complement(1750..2151)
                     /locus_tag="VMU21_07175"
     CDS             complement(1750..2151)
                     /locus_tag="VMU21_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77345.1"
                     /translation="MKHMIVIIVLSLLVFGVTPICSGAGPGEECATQFGGKCRTVCAS
                     NERSMQGAFLDCTEEEKCCVPGKTSAKHLKRVAGQVTAIDRGEKSITLEGITIMADEG
                     MLSNIKEGDRVTVDYYSRGVHRAIFILREVK"
     gene            2498..5686
                     /locus_tag="VMU21_07180"
     CDS             2498..5686
                     /locus_tag="VMU21_07180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO77346.1"
                     /translation="MKAPEITIIMPAYNHEQFVGEAIESVLSQTFENFELIIINDGST
                     DKTESVIKRYGDPRIRYYHQENKGSCHAHTRGIQLAGGKYISIIDSDDVYHPERLSVL
                     LDTAKNGDLDFLITDLNLIDQHSAIIDDPDHWWIKWYEHLKLAYRTSDSPDKALLSGN
                     FTITTSNFFFNSRLVQEIGAFKPYTYILDYDFAFRAARNRPGSFVFLDDRKLLSYRLH
                     GQNTILKNPLVANYETFYFLVNAIKDVFGPDMEIPVEHLNKIRRYLVKELTLRRKNDI
                     AAVRSDLTGALELKSAELAERSAELAQKSTELEFASQEVTRTVQELREQGRRLSDEEG
                     FNQRLLTEFYFIKNSFSFKIGRLFTLPLRTLRRNPLKPMKAEARDIRDLRTKLDPIVR
                     HVDVISFDIFDTIFERDIDPPDKVKEIVARRVAAILKEAYHITRSPLELLRLRDSIEL
                     KLRRDALKNGSDYECRYSDIVKEMVTVLLGEYDEKLYNEMIEEEIKVEDEVLYVKEGM
                     RELLEELRDRGKRIIAISDMYLDKEHVRRILQLKSLDFFFRDIYVSSESGICKYSGNL
                     FRHVLLRERVLPGQMVHIGDNRISDHRVPMRLGIHTIYLKDASHLKRKYVLKTYNRLA
                     AKNSYWKGRHLLQLVRPFKGREDFFYSYGFSFLGPVYSAFVYGVIETIKKQNIRKVYF
                     LAREGELFLRLFNTFESHFFEKGAAPDTEYVYLTRKSTALASAYQGLSHQKAIIALYN
                     PKQQGLQSICDVFGLPSGEFRDIAGQYGLTDMRRPLYDWHDERFKKLLMDGRLQDIVR
                     RHAGADRELLARYLEQIGFFNDSPVAFVDVGWNATIQKFVQDAFIERADYPHIYGLYL
                     GFIDGIKHAFEPQKNTIQGFFYDDLRNDPAEKILSRFAEIFEEGARAMHPTTIGYRHN
                     PGTGLVEPVFKNDRAYDRTVELLHNGKIAQIQSGALSFSMEFIRAVHLTGYSFEDIRP
                     FLLTLIERCIAFPRSEETKILMDLKHSEDFGYENVMDFTSDRINSLKTILHPGKFVNK
                     IKHSNWPYGTARSSGIPGINLGVRLYDLLWRP"
     gene            5692..5931
                     /locus_tag="VMU21_07185"
     CDS             5692..5931
                     /locus_tag="VMU21_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77347.1"
                     /translation="MNNSYLKKDLDIRRCGLPSVLLVALVHRVTFRIRRGHPLFSPRL
                     FEWAIALAAHRFFPIFHFGYTLRNLVRKIKARLRG"
     gene            complement(5965..6768)
                     /locus_tag="VMU21_07190"
     CDS             complement(5965..6768)
                     /locus_tag="VMU21_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO77348.1"
                     /translation="MSDTGSITDGRTVSFIIANWNHRQLLHECISSIYETAASLSLEI
                     IVVDNASADNSAEHIKKTFPGLVWVQNDINRGYAEAVNQGVKLSTGDFIFLLNNDVTL
                     AEHSVKMLVSFLAANPDAGAVAPRLYYPDGRKQISCRRFPTLPTLLMEYFGIDKMGHY
                     RRWELKEEEHLSTRIIQQPMASALMVKRKCWDSVGPMDEGFPILFNDVDWCYRLYANT
                     PYRIYLFPEAKAVHHKSASVNRLGYKKIVIFYRGLMRFYRKHFPFRSVW"
     gene            complement(7003..7974)
                     /locus_tag="VMU21_07195"
     CDS             complement(7003..7974)
                     /locus_tag="VMU21_07195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO77349.1"
                     /translation="MTESNVNPLVSIIVRTKDRPKLLMRALRSIAAQTYRPIEVVVVN
                     DGGCPLGAAELRSVLGDVSLNYLPLEQNTGRAHAGNVGIGNAKGEYVGFLDDDDEFYP
                     EHLGTLVASLSGSECNVAYAAVEFVDEVLNSEGTRVSSHLKGTFATPFSFDDLLIGNY
                     IPLISLLFRAATLRSFMLDESFELYEDWDMLIRAGEAAPFHFVDKITSIYYQRNDSQI
                     AFKSPPEVLREATVKLYEKHRAKLPLGTVFTMREKLYRKDGGTAQKEDRLRELEVRVA
                     GLESVAHDLRNILQERDSYIQSIHARRGWRLLTRYYRIKQWLLRLIR"
     gene            8263..9402
                     /locus_tag="VMU21_07200"
     CDS             8263..9402
                     /locus_tag="VMU21_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUO77350.1"
                     /translation="MTLFLLTFLLIYGSVHLYAFLKAKAALEFSAATGVFLVLFMLVM
                     GLAPLIVRALERTGLELFARVMAYIGYTWMGVLFLFFSYSLAAEIYRFLIFLGGAIFS
                     RDLSGLSLSPRYAFFLPLFLSLVTSLYGYFEARDIRTERWTLKSPKIPQEIGKLKIVQ
                     ISDVHLGLIVREERLKRILRLVKAENPDILVSTGDLVDGEVCRLNGLEDLLKEIKPRY
                     GKFAVTGNHEFFAGFENAKCFTENAGFTLLRGEGITVQGILNIAGVDDPSGKRYGLAK
                     EISEKILLSGLPQDKFTLFLKHRPIVDQDAKGLFDLQLSGHIHKGQIFPFSIITWLYY
                     PVNSGRMNLTDNAFLYVSRGAGTWGPPIRFLSPPEVAAIELVHGP"
     gene            complement(9414..10832)
                     /gene="mnmE"
                     /locus_tag="VMU21_07205"
     CDS             complement(9414..10832)
                     /gene="mnmE"
                     /locus_tag="VMU21_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HUO77351.1"
                     /translation="MFDDTIAAISTPLGEGGIGIVRLSGRDAIPIAEKLFSSPKNKKL
                     SGSESHTLTYGFIHDPAANTTIDEVLVSIMKAPNTYTKEDVVEINCHGGLLPLRKTLE
                     LVLRNGARLAEPGEFTKRAFLNGRIDLSQAEAVIDLIRAKTDESREAALEQLSGGLSE
                     KIVGLRERIASLCAHIEAYIDFPDEEIEPASMESIQNELRDILNALMLLSKSFEEGRF
                     FREGLKVAIVGRPNVGKSSLLNALLNRERAIVTEIPGTTRDVLEEYLNITGLPVRIMD
                     TAGIRETHEMAEKEGVARSLRAIGDADLVIGIIDGTNALNEEDFVVLEKIKEKKSIVA
                     LNKSDLPAAEDDLEKSLAPYSETVVRISAKTGDGLDLLKEAIIDVGIGHPVKASGERS
                     AIGQLEGNNGVIVTNLRHKVAIDNAFNALTNASAAIQSSLPLEIVAMEMRDGLDRVGE
                     VVGAVTTEDILNRIFSEFCIGK"
     gene            10906..11445
                     /locus_tag="VMU21_07210"
     CDS             10906..11445
                     /locus_tag="VMU21_07210"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HUO77352.1"
                     /translation="MARLFLIFKDFDWDEANIEHILRHNVVPFEVEEACINKPYVRKT
                     LEDRYLIYGTTDSGRYLFIVGIKKERGIFQGNNGKRDDREGKISLQKEKKMKKIPKFK
                     SEKEEAEFWASHDSTDFLDETKEVKVKFVRPKKKLVSLRLDEKTIKELKKLAQKKGIG
                     YLELVRMWVLENLGKTKAA"
BASE COUNT         3003 a         2855 c         2816 g         3034 t
ORIGIN      
        1 gcgacggtaa aagaatgtgc gaagaccctt gtaaaggcag gagcaaaaga agtggttgcg
       61 ataaccctcg cacgttctat gctggactag cgtgctgcac agcgttaccg tttctttcct
      121 gatataatga gggaaatacc ccgggaaagg agataccatg tttgtccctc ttctcaccgc
      181 actacatgtc actgttgtag tcctctggat tgggggcgtg gcctttgtca ccataattat
      241 ctttcccatg cttttgaaaa tggaagactc ctttgaaaag gttctccttt ttcagcgtgt
      301 ggagaacagg tttgcgaaac aggcgagggc atacgcatgg acagcaggaa tcacaggggc
      361 cattttgctc tacctcacag gacagcacag ggcattgttc accctgaatg gattgggagc
      421 aacggtcatg ctcatggtct ggctggtcta tacccttgtg ctgacatttg aaaaaaagat
      481 atttgcactc ttcttcagtc aaccagaaaa gctcgatgcg gcaaaaatct ttgtgagatt
      541 gaataccttc cattggttgg tcctcggatt gagccttgcc gcagtgttta tcggggtatg
      601 gacaggacac ggcggacatt tctaggcata ctattggcaa accagcttgg cgaggagaca
      661 tatccatgca gctaagaatt ctttttgggg ttttattctt gcttgtctct tttatcagca
      721 tcggaattgc tgaggaaaat caggaatcaa ggaatctcat tcagagggag atgattgccc
      781 tcgacagtgc cctcaagacc acaatcgatt ccctggtctt ggacgaaccg ggcaggatcg
      841 ggccggcctt tgatgaggtg gacaagataa gggagcaggt cgaatatgcg gtcgagcaca
      901 aggttaggat cacactgccc aggaaccaga agcgcttcaa agagtttgtc aggttggata
      961 ataaattcca tcatgaactt caaacccttt taaaggcagc aaagaaaaat aagatggggg
     1021 tggtccagaa gcagacacac agactcctcg atgcatgtgt cagatgccat gccatattca
     1081 ggaagtaggg gagttagtat cagagatcac acgatgaggt ggagattgaa aagatcgctt
     1141 tttattctcg tggcgggtat tttagtgaca acaaatgtct tttccgcgca gaagcatgaa
     1201 tgcagttatt gtcatgtcac ttctgataag actgttcggc agcaactcaa agcaccctta
     1261 tcttaattat gtgccgagtg tcatccagac agaaggagcc cgaatgaaca taaggttgat
     1321 cttgtgcctt tcatgaaggt tgtgggactt cctttgagca aggaggggaa gatcacctgt
     1381 gccacatgcc atgaccctca tgacactgga ggatatccca tgctgctgag agcaaggcca
     1441 gcagaacttt gttttaaatg tcatttcggg tagagttatt tgccaattga aggtcagata
     1501 aggaaaccgt caacaagcct tgttgattaa atgaggtttc caaaaactcg taatgaaaca
     1561 aaataaggcc taaagacgcc ttgagagaaa cagagggttt ttgcggtttc caccggcgaa
     1621 gtcaccgcta tttggaaagc gaagctctta acggtttcca ataggaataa gcataagata
     1681 ttgcgcacag gcgactctga gaataattga caaaagagcc gatatcaaaa caagtttctt
     1741 gaacttaact catttaactt ctcgcaatat gaagatcgct ctatgcacac ctcttgagta
     1801 gtaatctacg gtcaccctgt cgccctcttt tatgttggag agcatcccct catctgccat
     1861 tatcgtaatc ccctctagcg ttattgactt ttctccccta tcaattgctg ttacctgacc
     1921 tgcaactcgc tttaaatgct tcgcactggt cttcccgggg acgcaacatt tctcctcttc
     1981 tgtgcagtcg aggaacgcgc cttgcatcga cctttcattg gatgcacaga ctgtcctgca
     2041 ttttcctcca aattgagtag cgcattcctc acctggtccc gctccagaac atatcggggt
     2101 tacgccaaat actaaaagtg acaatacaat aattactatc atatgcttca tacgacatac
     2161 ctcctagcag tgtcggggac acatcccttt tcttgccccc actgcgagag tcagaacgat
     2221 ctccacttaa atcccttctc tgcttctgtt tgcactatac acctttgggg ctgggctttc
     2281 aagtgtggtt ttcaggaagg agttttgcct cgggcatcct taaaggaaaa ggcagatgaa
     2341 ttttataaga gcttttatga gaagtggaat gaggagattc cctttttttg ctgaggtgaa
     2401 caccctgtcc gcatcgttca tctcggcaga cattgctggc gatgttttgt tacaatagag
     2461 cccatagcaa gaaaaaacgt tgaacagata cttcccaatg aaagcccctg agatcactat
     2521 cataatgccc gcctataacc acgagcaatt cgtcggtgag gctattgaaa gcgtcctttc
     2581 ccaaacgttt gagaactttg aattgataat catcaatgac ggttccaccg acaagactga
     2641 atccgtgata aaaagatatg gcgacccgcg aatccggtat tatcatcagg agaacaaggg
     2701 ttcctgccac gctcacaccc gaggcataca gcttgccggg ggaaagtata tctctataat
     2761 agattccgat gatgtctatc atcctgaacg gctttctgtc cttcttgata ctgcgaagaa
     2821 cggcgatctt gactttctga tcaccgatct gaacctgatc gatcagcact ctgcaataat
     2881 tgacgatccg gatcactggt ggatcaaatg gtatgagcac ctcaagttgg catacaggac
     2941 atcagattca ccagacaagg ccttactctc agggaatttc acgattacga cttctaattt
     3001 tttcttcaat tcccggctcg ttcaagagat cggggcattc aaaccctata cgtatatcct
     3061 cgattatgac tttgccttca gggcagcccg gaaccgtccg ggaagtttcg tgttcctcga
     3121 tgacaggaaa ctgctctcct acaggttgca cggccagaat accattctca agaacccgct
     3181 cgtcgcgaat tacgagacgt tctatttcct ggtgaatgcg atcaaggacg tgttcggccc
     3241 ggatatggag atacccgtgg agcatctcaa caagataagg cggtatctgg taaaggagct
     3301 gacgctcaga cggaaaaacg atattgccgc agtcaggagt gacctgacgg gcgcgcttga
     3361 actcaagtcc gcagaactgg cggaaaggtc tgcagaactt gcacagaagt ccactgaact
     3421 cgagtttgcc tcgcaagagg tgacgaggac tgtccaggaa ctccgggagc agggtaggag
     3481 gctcagtgac gaagagggct tcaaccagag gctgctgact gagttctatt ttataaagaa
     3541 ttcctttagc tttaagatcg gaaggctctt caccctgcct ttgcggacgc ttagaaggaa
     3601 ccccctgaag ccgatgaagg cagaggcgcg agacatccgc gaccttcgta cgaagctgga
     3661 ccccattgtc cggcacgtcg acgtcatctc ctttgacatt tttgacacca tctttgaacg
     3721 ggatatcgac cctccggaca aggtaaagga aatagttgct cggcgtgtcg ccgcgattct
     3781 taaggaggcc taccacatca cccgctcgcc tcttgagctt ctcaggctga gggactccat
     3841 agagttgaag ctgagacggg atgcgttgaa aaacgggagt gactatgaat gccgttacag
     3901 cgatatcgtg aaggagatgg ttaccgtact tctcggcgag tatgacgaaa agctctataa
     3961 cgaaatgata gaggaagaaa tcaaggtcga ggatgaggtc ctctatgtaa aggaggggat
     4021 gcgagaactc ttggaggaac tgcgggacag agggaaaagg ataatcgcga tatcggacat
     4081 gtatctggat aaagagcatg tcaggaggat cctgcagctt aagtccctgg actttttttt
     4141 tcgtgatatc tacgtctcat ccgagtcggg gatctgcaag tactccggca atctcttcag
     4201 gcacgtcctg ttacgcgaga gggtcctgcc cggtcagatg gtccacatag gcgacaacag
     4261 aatatccgac catcgtgttc ccatgaggct cggaattcac acgatctatc tcaaagacgc
     4321 gtcacacctc aaaaggaaat atgttttaaa aacatacaac agactggcag cgaagaattc
     4381 ctactggaag ggaagacatc ttcttcagtt ggttcgtcct ttcaagggaa gagaagactt
     4441 cttttactcc tatggtttct ctttcctcgg acctgtctac tcggcgttcg tttacggggt
     4501 catcgaaaca ataaagaaac agaacatcag gaaagtctat ttcctcgcga gggaaggcga
     4561 gcttttcctc cgcctcttca acacgtttga aagccatttc tttgaaaagg gagctgcacc
     4621 ggatacggaa tacgtctacc tcacaagaaa atccaccgca cttgcatctg cctatcaggg
     4681 gctttctcac caaaaggcga ttatcgcgct ctacaatcca aagcagcagg gactgcaatc
     4741 catatgcgac gtcttcgggt tgccctccgg ggagttcagg gacatcgccg gacagtatgg
     4801 cctcaccgac atgagaagac ccctatatga ctggcatgac gagcgcttca agaagctgtt
     4861 gatggacggg aggttacagg atatcgtcag gagacatgcc ggcgcagaca gggaacttct
     4921 cgcacgttat ctcgaacaaa tcggattttt caatgacagc cctgtggctt tcgtggacgt
     4981 aggatggaat gcaacgatcc agaaattcgt ccaggacgct tttatcgaaa gagctgatta
     5041 ccctcacatc tacggcctct acctgggctt cattgatggg atcaagcatg ccttcgagcc
     5101 gcaaaaaaac acgattcagg gcttcttcta tgatgatctc cgtaatgacc ctgccgaaaa
     5161 gatactcagc agatttgcgg agatatttga ggagggagcg cgcgccatgc accccacgac
     5221 gatcgggtat agacataatc cggggacggg actggttgaa cccgttttca agaacgatcg
     5281 ggcatatgac agaacagtag aacttctgca taacggaaag atagcgcaga tccagagtgg
     5341 ggctctcagt ttctcgatgg aattcatcag ggctgtccac cttaccgggt attcctttga
     5401 agacatcagg ccttttctcc tgaccctcat cgagcggtgt atcgcgttcc cgcgaagcga
     5461 agagacaaaa atcctgatgg acctgaagca ttcagaggat ttcggttatg aaaatgtcat
     5521 ggacttcact tcggacagaa taaacagtct taaaaccatc ctccatccgg gcaaattcgt
     5581 gaacaagatc aaacattcga actggccgta cggaacggca aggtcgtccg gcatcccggg
     5641 aatcaacctg ggcgtgagac tgtatgatct gttgtggagg ccctgagaac tgtgaacaat
     5701 tcctatttaa agaaggacct ggatattcgc cgatgcggtc ttccttctgt cctgcttgtt
     5761 gccttggttc atcgggtaac gttcaggatc agacgtgggc atcctctttt ttctccacga
     5821 ctcttcgagt gggcaattgc tttagcagcg caccggttct tccccatatt ccattttggt
     5881 tataccctca ggaacctagt gaggaagatc aaggcaaggc tgcggggcta acaagtcctt
     5941 ccggggtgtt cccgcaacaa gcagttacca gacggaacga aagggaaagt gcttccgata
     6001 gaacctcatg agcccccgat agaatattac tatcttttta tatcccaacc ggttcaccga
     6061 agcgctctta tgatggaccg cttttgcttc ggggaaaaga tagatcctat agggagtatt
     6121 cgcataaagc ctgtagcacc agtcgacgtc attgaaaaga atgggaaagc cttcgtccat
     6181 aggtcccacc gaatcccagc atttcctctt gaccattagg gccgacgcca taggctgctg
     6241 tataatcctc gtactaagat gttcctcttc cttcaattcc caccgccgat aatgccccat
     6301 cttgtcgata ccgaaatatt ccatcaagag cgtcgggagt gtcgggaaac gtctgcatga
     6361 tatctgtttt ctcccgtcgg ggtaatataa cctgggtgca accgctccag cgtcaggatt
     6421 ggcggcaaga aaggagacaa gcatcttcac gctgtgttca gccagggtga catcgttgtt
     6481 cagaagaaag ataaaatccc ctgttgacaa tttgaccccc tggttgactg cctccgcata
     6541 tccgcgattg atatcgttct gtacccacac cagcccagga aaggttttct tgatatgctc
     6601 ggcgctgttg tctgccgacg cattgtccac cacgattatc tcaagagaga gagaggcggc
     6661 agtttcgtat attgaagata tgcactcgtg gagcaattgc cggtggttcc aattggcgat
     6721 gatgaaggaa acggtgcgac catctgtgat cgatccggtg tcactcatct gcatcccccc
     6781 ttcgaccgca ggaagtgaat tctgccacga atgatgatag cctctccagt aattgagatt
     6841 tttttctttt cttaatcaga ttcagagact cttcgctcgg tttccgttaa tagcagatgt
     6901 gccgcctcaa tacttctttg agcctcctgc aaattgagca atcgtcttgc cccctcttta
     6961 taggacattt gctttcatga gcgttcacac ttcgatgagc gcctaccgta tcagccgaag
     7021 aagccactgt tttattctat aatacctggt caaaagtctc cagccccgcc tggcatggat
     7081 gctctgtata tacgaatctc tttcttgcaa aatgttcctg agatcatgtg cgacactttc
     7141 gaggccggcg acgcggacct caagctctct tagacgatct tctttttgcg cagtcccgcc
     7201 atcctttcta tagagtttct ccctcatggt gaacacggtg ccgagaggaa gttttgcccg
     7261 gtgtttctcg tagagtttca cggtcgcctc cctcaatact tcaggagggc tcttgaacgc
     7321 tatctgcgaa tcgttcctct gataatatat tgacgtaatt ttgtcaacaa aatggaaggg
     7381 cgctgcctct ccggccctta tgagcatatc ccaatcctcg tagagctcaa aggactcatc
     7441 gagcatgaag ctcctgaggg tggctgccct gaataacagg gagataagcg gtatgtagtt
     7501 tcctatgaga agatcgtcga atgaaaacgg tgtcgcaaat gttcctttca ggtgactgct
     7561 tacacgggtc ccctcactgt tcagtacttc atccacaaac tcgactgcgg cataggcaac
     7621 attacactct gatccactca aggaagcgac aagggttccc agatgctcag gatagaactc
     7681 gtcgtcatcg tccaggaacc cgacatattc ccctttggcg tttccgatgc ccacgttccc
     7741 tgcatgcgcc ctccccgtgt tctgctccag cggcaggtaa ttcagggata catcgcccag
     7801 gacgcttctc agctctgctg cgcccagggg gcagcccccg tcattcacca ccacgacctc
     7861 tatcggccgg taggtctggg cagcaatgct ccggagcgcc ctcatcagga gcttcggcct
     7921 gtcctttgtc cttacgatta tggaaactaa tgggttgacg tttgactcag tcattttttc
     7981 gggcgttgaa aaggatttta tattttaccc aaaaagggtc ccctattact atgccattcc
     8041 taagaacatg gacaagtatt ctatgacgtc attcgaacag ttctaatgac gattcgcata
     8101 ccgggatctc gccggcaaca aacagggtcc catctcacga ttgacatatc gtcagaccat
     8161 aagaataaac atgctctcct atctggtata attttctcat gcgtaaggct tctttaagga
     8221 aaaatcataa aacgtggctg ggataatcaa cgggagtttt tcatgaccct ttttctgctc
     8281 acctttctcc tcatttatgg gagcgtgcat ctctacgcat tcctgaaggc aaaggctgcc
     8341 ttagagttta gtgctgcaac cggtgtcttt cttgtcctat tcatgcttgt gatgggattg
     8401 gcccccctca ttgtgcgtgc attggagagg accgggctgg aattattcgc gcgggtcatg
     8461 gcatacatcg gctatacatg gatgggagtg cttttccttt tcttctccta ttccctcgca
     8521 gctgaaatct atcgctttct catattttta ggcggggcga ttttcagcag ggacttgtcc
     8581 ggcctgagcc tctcccccag atatgcgttc tttcttcccc tttttctctc actggttacc
     8641 tcactctacg gttattttga ggcgagggac atccgcacag agagatggac actcaaaagc
     8701 cccaagatcc ctcaggagat aggaaagctg aaaattgtcc agatctctga tgtgcatctc
     8761 ggtctgatcg tgagagaaga gagattgaag aggatactcc ggctggtgaa agcagaaaac
     8821 cctgatatcc tggtttccac cggcgacctt gtggacggag aagtatgcag actcaacgga
     8881 cttgaggacc tgctcaagga gatcaagccg agatacggga aatttgcggt aaccgggaat
     8941 catgaatttt ttgcagggtt tgaaaacgca aagtgcttta cagaaaatgc gggcttcacc
     9001 cttctgaggg gagaaggaat tacggttcag ggaattctca acattgcggg agttgatgat
     9061 ccctcaggaa agagatacgg actcgcaaaa gagatttcag agaagatttt gctctcaggg
     9121 ctcccgcagg acaagtttac gctttttttg aaacacagac ccattgtgga ccaggatgca
     9181 aagggacttt tcgatcttca gctctcaggg cacattcata aggggcagat attccccttc
     9241 agcatcatca catggctcta ttatccggtc aattcaggaa gaatgaatct cacggataac
     9301 gcgtttttgt atgtgagcag gggtgcgggc acgtggggac ctcccatccg ctttttgtca
     9361 ccgcctgagg ttgcagccat cgaacttgtc cacgggccct gatcagggga tacttacttc
     9421 ccgatacaga attcgctgaa tatcctgttc aggatatctt ctgtggtgac cgcaccaacg
     9481 acctctccca ccctatcgag accatcacgc atttccatgg caacgatttc cagaggaaga
     9541 gatgattgaa tcgcagcaga ggcattagta agggcattga aggcattgtc aatggcaact
     9601 ttgtgcctga ggttggtgac tatgacccca ttgttcccct ccaattgacc aatagcgctt
     9661 cgctccccgc ttgccttgac aggatgtcca atgcccacat cgatgatcgc ttctttgagg
     9721 aggtcaagtc cgtcacccgt ctttgcagaa atcctcacta cggtctcgga gtagggagca
     9781 agactctttt caagatcatc ttcagcagcg ggcaggtcag acttgttgag tgcaacaatg
     9841 ctttttttct ctttgatttt ttcgagaacc acgaagtctt cttcattcag ggcatttgtt
     9901 ccgtcaatga tgcctattac caaatctgca tcaccaattg ccctgaggct tctcgcaact
     9961 ccttcctttt ctgccatctc atgggtctcc ctgatccctg cagtgtccat tatccttaca
    10021 ggaagaccag taatgttgag atactcctca aggacatccc tcgtggttcc ggggatctcg
    10081 gtcacgattg ccctctcacg gttgagaagc gcattcagga gtgaagactt tcccacattc
    10141 ggtcttccca ctatggcgac cttaagccct tccctgaaga aacggccctc ttcaaagctc
    10201 tttgagagga gcatgagggc attgagaata tcgcggagct cgttctggat gctctccatt
    10261 gatgctggct cgatctcctc atcagggaaa tctatgtatg cctcgatatg agcgcagagg
    10321 gatgcgatcc tttcccttaa tccgacaatt ttctcggaaa gacctcccga gagctgttcc
    10381 agtgctgctt ccctcgactc gtcggtcttt gcccgtataa ggtctatgac tgcctctgcc
    10441 tgcgacaggt ctatcctgcc gttgagaaat gcgcgcttcg tgaattctcc cggttctgcc
    10501 agccgggcgc cgtttcttag taccagttca agggtcttcc ttagaggaag aagaccgcca
    10561 tggcagttta tctccaccac atcttccttt gtataggtgt tcggtgcctt cattattgag
    10621 acaaggactt cgtcgatggt cgtattggct gcagggtcat gtatgaatcc ataagtgagt
    10681 gtgtgtgatt cagatcctga gagtttttta ttttttggag atgaaaaaag tttctccgct
    10741 atagggatgg catccctgcc gcttaatctc acaatgccaa tgcctccctc tcccagggga
    10801 gtagagatag ctgctatggt gtcgtcgaac ataaagtatt atacccaaga agggtaatct
    10861 cttgacacag gctgatataa gagctatctt atagacatct aatagatggc gaggctgttt
    10921 ttgatcttta aggactttga ctgggatgaa gcaaacattg agcatatact gcggcacaat
    10981 gtggttccat tcgaagttga ggaagcctgt atcaacaaac catatgtcag gaagactctt
    11041 gaggaccgct atctgatata tggcacgacc gattccggca ggtatctttt cattgttggc
    11101 atcaaaaagg aacgcggaat ttttcagggc aataacggga agagagatga ccgagaggga
    11161 aagatctctt tacagaagga gaagaaaatg aaaaaaatac caaaattcaa atcagaaaag
    11221 gaagaggctg agttttgggc gtcccatgac agtacagact tcctcgatga aaccaaagag
    11281 gtcaaggtta agtttgtgcg gcccaaaaag aaactggtct cgctcagact tgatgaaaag
    11341 accatcaagg aactgaagaa actcgcacag aagaaaggca tcggctatct ggaactggtg
    11401 aggatgtggg tgctcgaaaa tttggggaaa acgaaagccg cctgatctgc taccagcctt
    11461 gcctgcatgg cagctaaggt gttgtcgtgc ataatgtatt ataagcaatc tctcattgcc
    11521 attattattg acacatcact tgaattcctc taaaatcctt acaaaaagac gcatgcggca
    11581 taaaaaataa tgttatgcat ggggaaaaat agaatgagca atattaagtc gttcgaagat
    11641 ctttttaaaa gcatccaagc gtgcaagctc gccaagactt gctcagatcg taacgtccgt
    11701 caatacta
//
LOCUS       DATKOU010000158         7296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2528614, whole genome shotgun sequence.
ACCESSION   DATKOU010000158 DATKOU010000000
VERSION     DATKOU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528614"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VMU21_07215"
     CDS             <1..537
                     /locus_tag="VMU21_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77353.1"
                     /translation="FAKEKAGVKVEQMADVYKMLVEKLHPLSIGNAYRAYRMAKLLTE
                     RLLSLHMDKKKDSEKMERIIKEITGDITIHAYPIDRDEAKELGLKVDIPKDSVEQLLW
                     QLYEEYATPMKLGQPFHPAELLAGKEMAEIRHVGAFIESAELSHQFTFTGKVQKTIRN
                     NQPVVDMNIDSQSWVKIE"
     gene            542..898
                     /locus_tag="VMU21_07220"
     CDS             542..898
                     /locus_tag="VMU21_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77354.1"
                     /translation="METTLYNKEMLTDTSSVLLGTLGVDEISLTPVNISRFKRLDEWG
                     NMILIFPSWEHSQPKVDVKMVLPLRLTDPLYGREELNHKLQEMATGAPSVEQVWEITK
                     RLPSLSRLLSEERDNE"
     gene            899..1372
                     /locus_tag="VMU21_07225"
     CDS             899..1372
                     /locus_tag="VMU21_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO77355.1"
                     /translation="MPKYFLESSAFIKRYKSEIGSDVVNSLFNNNHDLFYLNLAIVEI
                     RKVFYRLWKYPLHQDDQISENEYKALVSRFATDLLQMQRIVFTEEMIAQTDSILERVW
                     VPNIFDFAQLSAYLIAKQEYPDMIFVCSDERSKLIDAVKQFVPTNDFIIPGRLAK"
     gene            1575..1778
                     /locus_tag="VMU21_07230"
     CDS             1575..1778
                     /locus_tag="VMU21_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77356.1"
                     /translation="MTKAEIKKVQQGLEKWQESHADINLLRKAYREKILDWVVKSMEF
                     EKEPVSMSRLKELLEREKAAHVR"
     gene            2055..2378
                     /locus_tag="VMU21_07235"
     CDS             2055..2378
                     /locus_tag="VMU21_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HUO77357.1"
                     /translation="MKILPFDSPGSHKSGIFKKFSETLAYADWRFQWIHPFKDFNGRV
                     GRILLSAILFKLRLPTAETASVDSQGKKRYLKALHEADAGDLSYLNEIWIERLSDAIK
                     ESEEK"
     gene            2375..2893
                     /locus_tag="VMU21_07240"
     CDS             2375..2893
                     /locus_tag="VMU21_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77358.1"
                     /translation="MSFQETAKWVEEELKAGRNDTVQDFLAYLAEQMIEMNKTKNEEI
                     KGFLKWLAHETGVEIDTLSNKTVIKEYHSNNFEQFLEVLKRNKKKISIDASDRKKQEL
                     LENNFSKSMLVLEPLKEKLKSTDNLIDEIVYRLYGLTDEEIEIVEGGNHKSQKEGGRK
                     APYPVGRGQDNE"
     gene            2929..3150
                     /locus_tag="VMU21_07245"
     CDS             2929..3150
                     /locus_tag="VMU21_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HUO77359.1"
                     /translation="MGTISDELAAKIKSLPDSEKMELIDSILTQLDKPDPEMDRIWAD
                     EARKRWDAYKKGKLKTVPYDQVMAKHRSG"
     gene            3239..4147
                     /gene="ftsY"
                     /locus_tag="VMU21_07250"
     CDS             3239..4147
                     /gene="ftsY"
                     /locus_tag="VMU21_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HUO77360.1"
                     /translation="MMGFFDRLKEGLTKTRKGFIGKVESIFTGRKIDEETLEELEETL
                     ISSDIGVKATTEIVEFLRDKAKKGEIKDSHNVRDFLKEEMAILLGNSQPLVITGEKPF
                     VILTVGVNGVGKTTTIGKLASRFVSEGKTVLLAAADTFRAAAIEQLEIWAKRTDSQIV
                     KHQSGSDPAAVAFDSIEASQSRNVDVVIIDTAGRLHTKSPLMEELKKIRRVIDKALPG
                     APHETLLVVDATTGQNALRQAEMFHNVVGVTGIALTKLDGTAKGGIVFAIRRELGIPI
                     RLIGVGEGVEDLRDFDSKEFVEALFS"
     gene            complement(4101..6002)
                     /locus_tag="VMU21_07255"
     CDS             complement(4101..6002)
                     /locus_tag="VMU21_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HUO77361.1"
                     /translation="MPESENIRDQRTHPQREMGGSRTPDPFLRKTYRSRRFRLLLVLL
                     GSLSILFGVSGGFLYWSLSDLPAVKSLEGYSPVQSSLVYSSDGKLLAELYIERRTFIP
                     YYKIPGHVKKAFVAVEDVRFYSHPGVDVIGIMRALYQDIRTMSIAQGGSTITQQLAKM
                     LFLKPERSIMRKLKEAAISIQIEKHYTKDEILGLYLNQAYFGTRAYGIEAAAQTYFGK
                     PVSALTVGEAALLASLPKAPSLYSPFKYPEKALERRSTVLKMMLHSKFITEAQYEAAE
                     KEPIPVTPHFRKYEAPYFIEMLRQDLETHYGNELYTAGYRIYSTIDFTMQKAAEEAVH
                     NGIISLEKRAKKGVQAALIAMEISTGRIKALVGGSDFWTNQFNRATHALRQPGSAFKP
                     FVYLTAIEHGMSADDLVVDAPITLKGARPGHPWVPKNYDGKYYGTVTLKTALAKSLNA
                     ATIRLAERTGVGNIIETAQRLGIKSNLQPYLPIALGASDVILMDMVSAYGTFATGQYV
                     KPLLYDRIISKDGLVLNELTTETTDLVSSEDVEQMRRLLHAVVEEGTAQKAKELKRTL
                     YGKTGTTNDYTDAWFMGFDDRLVVGVWVGRDDHTPLGAKETGARAALPIWMEFMKKAP
                     LQTPLNQNP"
     gene            6128..>7296
                     /locus_tag="VMU21_07260"
     CDS             6128..>7296
                     /locus_tag="VMU21_07260"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HUO77362.1"
                     /translation="MGKDKITVGIIGLGTVGTGTVRILLDNKAIISERIGFDIVLKRI
                     ADKDVHRDRGIVLPKGMLTQNAEEILNDPEIDIVVELIGGIHPAKEFILGAIQRKKHA
                     VTANKALLATEGNEIFSEADKAGVGVGFEASVAGGIPIIKVLREGLVANNVVAIYGII
                     NGTSNYILTKMTNEKVEFSEALREAQRLGYAEADPTFDIEGIDSAHKLTILSSLAYGI
                     PLSFEEVYKEGITKITSQDIEFASELGYKVKLLAITKASDGGIEMRIHPTMIPSEYLI
                     SKVDGVFNAVYVEGDAVGSTLYYGRGAGDMPTGSAVVADIVDIGRTITSGGSQRAAGI
                     PGVWTKGAKKRKIKKMEDVESMYYFRFSALDKPGVLSKISGILGNYNISIASVIQ"
BASE COUNT         2137 a         1489 c         1898 g         1772 t
ORIGIN      
        1 tttgcgaaag agaaagccgg cgtaaaagtc gaacaaatgg cagatgttta taaaatgctg
       61 gttgaaaaac tgcatccatt gtctatcggg aatgcatacc gtgcatatcg gatggctaag
      121 cttttgactg agaggcttct atctcttcat atggacaaga aaaaagacag tgaaaaaatg
      181 gaaagaatca taaaagaaat tacaggagat attactatac atgcatatcc tattgatcgg
      241 gacgaggcaa aagaactcgg actaaaagta gatattccaa aagactctgt tgagcaactt
      301 ctgtggcaat tgtatgagga gtatgctacg cctatgaaac tcggtcagcc atttcatcct
      361 gccgagctcc tcgccggcaa ggaaatggct gagatacggc atgttggagc atttatagag
      421 agtgcggaat tatctcatca gtttactttt accgggaaag tacaaaaaac tattcgtaat
      481 aatcaaccag tggttgacat gaatattgat tctcagtcat gggttaagat agaataaagt
      541 tatggaaacg acattatata ataaggaaat gctgacggac acgtcatctg tattgttggg
      601 aactttaggc gtagacgaga taagtctaac acctgtgaat atatcaagat ttaaacggtt
      661 agatgaatgg gggaatatga tattaatatt tccctcctgg gagcacagtc agcctaaagt
      721 tgatgtgaaa atggttctgc ctttgcggct tactgatcca ctatacggcc gcgaagagct
      781 aaatcataaa ttacaagaaa tggccaccgg cgctccctcc gtagaacaag tatgggaaat
      841 aacaaaaaga ttgccgtcat tatcgagact cctctcagag gaaagagata atgaataaat
      901 gccaaaatac tttcttgagt caagcgcctt tattaagcgt tacaaatctg aaataggttc
      961 tgatgtagta aacagccttt ttaataataa ccacgattta ttttacttaa accttgcgat
     1021 tgttgagatc cgcaaggttt tttatcggtt atggaaatac cctcttcatc aggacgatca
     1081 aattagcgaa aatgaatata aagccctcgt cagcaggttt gctacggatc tccttcagat
     1141 gcagagaatc gtatttacag aagaaatgat tgcacaaacg gattctattc ttgaaagagt
     1201 ctgggtccct aatatttttg attttgcaca gctttcagct tacttgattg cgaaacaaga
     1261 ataccctgat atgatttttg tctgttcaga tgaacgttca aaattgatag atgcggtgaa
     1321 gcaatttgtg ccgaccaatg actttatcat ccctggacgt cttgcgaaat agaaaggata
     1381 tccagaaagt atgcaaagca aaaaaggggg cgggtacatt atctatcgag cagaggcaag
     1441 caggattatt gaggaagcca aaaagagaac tgaagatatg atattgggag attgagattg
     1501 tggagggaaa agaaaaagcg aaactattgc aatgaaaact aataaatgtc aaaataaaaa
     1561 gaaggaagga gaaaatgaca aaggcagaga taaaaaaagt ccagcaaggg ctggaaaagt
     1621 ggcaggaaag tcacgcagat ataaacttgc tcaggaaggc atatcgagag aagatactgg
     1681 actgggtggt gaagtccatg gagttcgaga aggagccggt gagtatgagc cgcctgaagg
     1741 aactcctcga aagagagaag gcagcacacg tccgttagat gcaacccgct attttgaaac
     1801 ggcgcagggt ataaagacat attctgaagt agcggaaatc cttgccgtat cagtcgcaaa
     1861 aagcatagaa ctaattactg atgggattcc tgaagatatt cacattaccc cagagtggat
     1921 atgcaaaatt catagcgaca tagcaggctc attgttccct gactgggcag ggaggttcag
     1981 agatgtaaat gtccaggtag gctcacatgt ccctccgcca tatttcgaag tccctgttca
     2041 catgcgcttg tattgtgaag atcttgccgt tcgactctcc ggggtctcac aaaagcggga
     2101 tatttaaaaa attctcagag acccttgcct atgcagactg gcggtttcag tggattcatc
     2161 catttaagga tttcaatgga cgtgtcggca ggattctatt atccgcaata cttttcaagc
     2221 taagacttcc gaccgctgag actgcgtcag ttgactctca agggaagaaa cggtacctga
     2281 aggcgctcca tgaggctgat gcaggggatt tatcgtattt gaacgagata tggatagaaa
     2341 gactttcgga cgcaataaag gagagcgaag aaaagtgagt tttcaggaaa cagcaaagtg
     2401 ggttgaagag gaattaaagg ctggcaggaa cgacaccgtc caagattttc ttgcctatct
     2461 tgccgagcaa atgattgaga tgaacaagac gaagaatgaa gaaatcaagg gctttctcaa
     2521 atggctggca catgagaccg gcgtagagat cgacacgtta tcaaacaaaa ctgtgatcaa
     2581 ggaatatcat tcaaataact ttgagcagtt tcttgaagtc ctcaaaagaa acaagaagaa
     2641 gatttccata gacgcttcag accgcaagaa acaggaactt ctcgaaaata atttttcaaa
     2701 aagcatgttg gttctggagc cgttaaaaga aaaacttaag agcaccgata atctgataga
     2761 tgagatagtt tatagattat atggtttgac tgatgaggag attgagattg tggagggggg
     2821 aaatcataag tcgcagaaag aaggtggacg aaaagcacct tatccggtgg gcaggggaca
     2881 ggataacgag taagcataag tggtaacata taacaaagga ggaacattat gggaactatc
     2941 agcgatgaac ttgcagcaaa gattaaatct ttgcctgaca gcgaaaagat ggaactgata
     3001 gattctatct taacgcaact tgacaagcct gatcccgaaa tggaccgcat ctgggcggat
     3061 gaggcacgca aacgctggga tgcatataaa aagggcaaat tgaagacagt gccatatgat
     3121 caggtgatgg ccaagcatcg ctccggatga aggtccactt cctaaaaccc gcccaggatg
     3181 aaataagaaa cgtcaagaag tactttcaga aagaattaat tttccattaa aaggagaaat
     3241 gatgggattt tttgacaggc tgaaggaagg actcaccaag accagaaagg gattcattgg
     3301 gaaagtagag tccatattca ctgggagaaa gatcgatgaa gagacccttg aggaactgga
     3361 agagacgctc atctcctctg atatcggggt aaaggcaact acggagattg tggagtttct
     3421 cagggacaaa gcaaagaaag gtgagataaa ggattctcat aatgtgaggg attttctcaa
     3481 ggaggagatg gcaattcttc tggggaattc ccagcccctt gtcatcacag gagaaaaacc
     3541 ctttgtcatt ctgacggtag gagtgaacgg ggttggcaaa acgaccacta tcggaaaact
     3601 cgcaagcagg tttgtgtcag agggaaaaac cgtcctcctt gccgcagcgg ataccttcag
     3661 ggctgctgcc atagagcagc tcgagatctg ggcaaagagg acagactcac agattgtgaa
     3721 acatcaaagc ggttctgatc ctgctgcggt tgcctttgat tccatagagg cctcacagag
     3781 caggaatgtg gatgtggtga tcatagacac cgcaggcagg ctgcatacaa aaagccctct
     3841 catggaggag ttgaagaaga taaggcgggt gatcgataag gcccttcccg gtgctcccca
     3901 tgaaaccctc cttgtggtgg atgccaccac agggcaaaac gccttaagac aggcagagat
     3961 gtttcacaat gtcgtggggg tgaccggtat cgcgctcaca aagcttgatg gtactgcaaa
     4021 aggagggata gtgtttgcca tcaggagaga gcttgggatt cccatacggc tcatcggcgt
     4081 gggtgaggga gttgaggacc tcagggattt tgattcaaag gagtttgtag aggcgctttt
     4141 ttcataaatt ccatccagat ggggagggct gcgcgggcac ccgtttcctt tgcacctaag
     4201 ggagtatggt catcccttcc cacccagacg cccaccacga gcctgtcatc aaagcccata
     4261 aaccatgcgt cggtgtagtc attcgtggtg cctgttttcc cataaagcgt cctcttaagc
     4321 tcttttgcct tttgggcggt tccctcttcc accaccgcat ggagcagtct cctcatctgc
     4381 tccacatctt cggaggatac aagatctgtc gtttcagtcg tgagttcatt aaggacaagg
     4441 ccgtctttgc tgatgatccg gtcatagagg aggggtttca cgtactgtcc tgtggcgaaa
     4501 gtcccgtatg ctgagaccat atccatcagg atcacatccg acgcgcccag agcaatgggg
     4561 agatacggct ggagattact ctttatgccg agcctctggg ccgtctctat gatgttgccc
     4621 acgccggtcc tttccgcaag ccgtattgtt gccgcattga gggattttgc gagggctgtc
     4681 ttgagggtaa cggtcccata gtattttccg tcgtaatttt tgggaaccca ggggtggcct
     4741 ggtcttgcgc ctttcaatgt aatgggggca tccaccacca gatcatcggc gctcatgccg
     4801 tgttctatcg cggtcaagta cacgaagggt ttgaatgcag agcctggctg cctcagcgcg
     4861 tgagttgcgc ggttaaactg gtttgtccag aagtctgaac ctcccaccaa ggctttgata
     4921 cggcctgtgg agatctccat tgcaatgagt gcagcctgta cccccttctt tgccctcttc
     4981 tccagagaaa tgattccatt gtgcaccgcc tcctctgctg ccttctgcat agtaaagtca
     5041 atagtggagt aaatcctgta ccctgcggta taaagttcat tgccgtagtg cgtctccaga
     5101 tcctgccgca gcatctcgat gaagtagggg gcctcgtatt tgcggaaatg aggagtcaca
     5161 ggaatcggtt ccttctccgc agcctcatac tgtgcctctg tgataaattt gctgtgcagc
     5221 atcattttaa gtaccgtaga tcttctttca agtgcctttt ccggatactt gaaaggagaa
     5281 tagagggagg gggctttggg aagagaggcc agaagggcag cctcacctac cgtgagggcg
     5341 ctcaccggtt ttccgaaata ggtctgcgct gccgcctcta tcccgtatgc cctggtgcca
     5401 aaatacgcct ggttcagata cagtccgaga atctcatcct tggtatagtg cttctctatc
     5461 tggatcgaga ttgcagcctc cttcagcttc ctcatgatgc tcctctccgg tttgagaaaa
     5521 agcatctttg cgagctgctg ggtaatggtg cttcctccct gggcgatact cattgtcctg
     5581 atgtcctggt agagcgctcg catgatcccg atgacatcga ctccgggatg gctgtaaaat
     5641 ctcacgtctt ccacagcaac aaacgccttt ttcacatggc cgggtatctt gtaataggga
     5701 ataaatgtcc ttctctcgat gtagagttct gcaaggagtt tgccatccga ggaatatacc
     5761 agggatgatt gtacagggga atagccctcc agagatttaa ctgccgggag gtcagaaagg
     5821 ctccagtaca ggaagcctcc ggagactcca aagagaatgg ataaggagcc gaggaggacg
     5881 agtagcaatc tgaaacgtct cgaacgatag gtctttcgca gaaagggatc cggagtccgt
     5941 gagcccccca tttccctttg ggggtgcgtt cgctggtcac ggatattctc tgattctggc
     6001 attattttta ttataccgca agtatgatga tgaaaggaga agtggcagcg tgtattgaag
     6061 aagcatgagc gtttcaagca taatatataa aattcttaca taggacagag cgaggagata
     6121 gatcggaatg ggaaaagata agattacggt cggaatcatc ggtttgggta ctgtaggtac
     6181 tggaacagta aggatactgc tggataataa agccattatc tctgaaagga ttggctttga
     6241 tattgttctc aaaagaattg cggacaagga tgtccatagg gacaggggga ttgtacttcc
     6301 caagggcatg ctcacccaaa atgccgagga aatactcaac gatcctgaaa tagacattgt
     6361 ggtagagctt atagggggaa tacatcctgc aaaggagttt atattagggg cgatacagcg
     6421 taagaagcat gcggtgactg ccaacaaggc actccttgcc actgagggaa acgagatatt
     6481 cagcgaggca gataaggctg gagtcggggt ggggtttgag gcatcagtcg ctggagggat
     6541 accgatcata aaggtactca gggaagggct agttgccaat aacgtggttg caatatacgg
     6601 gattataaac gggacctcca attacatcct cacaaagatg accaatgaga aggtggaatt
     6661 ctcagaggca ttaagggaag cccagaggct cggctatgcc gaggctgatc ctacctttga
     6721 tatcgaaggc attgattcag cacataaatt aaccatcctc tcatctctcg catatggaat
     6781 ccccctttcg tttgaagagg tgtacaaaga agggattaca aagataactt cccaggacat
     6841 tgaatttgcc tcggaactgg gatataaggt gaagctcctt gccatcacaa aggcctctga
     6901 tggagggata gagatgagga tacaccctac catgataccc agcgaatatc tcatctcaaa
     6961 ggttgacggt gtctttaatg cagtgtatgt tgagggagac gcagtgggtt cgaccctcta
     7021 ttacggaagg ggggcaggag atatgcccac agggagtgcg gttgtggccg acatagttga
     7081 tatagggaga accataacct caggcggctc ccagagggcc gcgggaattc cgggagtgtg
     7141 gacaaagggg gccaagaaaa ggaaaatcaa gaagatggag gatgtggaga gtatgtacta
     7201 cttcaggttc tctgccttgg ataagccggg ggttctgtca aagatatcag ggattctcgg
     7261 caattacaat ataagcattg catcggtgat tcagaa
//
LOCUS       DATKOU010000159        19027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5609061, whole genome shotgun sequence.
ACCESSION   DATKOU010000159 DATKOU010000000
VERSION     DATKOU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5609061"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..275
                     /locus_tag="VMU21_07265"
     CDS             39..275
                     /locus_tag="VMU21_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HUO77363.1"
                     /translation="MFGGQVEMVKLIKDLKLKVNAEIQKDQVRVRAKKNRRSPDDYGQ
                     TEGRRLRDTSPVHELQIVAAKQSLFSPLPFLISV"
     gene            401..730
                     /locus_tag="VMU21_07270"
     CDS             401..730
                     /locus_tag="VMU21_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77364.1"
                     /translation="MKRRILRIFIICVTMSHLGSSISTAADVKVMYAIGLLDYGENAR
                     LVTTEEFNQTVANYEKFRDRVAKAPTLKAMVQNGWTIVHVEQIRAGDGDPYRELNYLI
                     IFQGGRR"
     gene            complement(979..1575)
                     /locus_tag="VMU21_07275"
     CDS             complement(979..1575)
                     /locus_tag="VMU21_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HUO77365.1"
                     /translation="MIARISVTVFFFLSSLLPALIIGENVLADEAIPFTERGSLKYVT
                     VQINGVPVELVFDTGSNSIVLNSAALQSLGIIEFSDARKIQAHTAGGIVEGYVLTLNS
                     IVIGSIRGYNYDVAYVPSSSENLLGSSFFASYNYYIDEDYKVIRLIPKGSFIFDSPWQ
                     PPPSVELQRTGSGRIEVEMDGKKFIFGEGWQDSEGPAK"
     gene            complement(2643..2786)
                     /locus_tag="VMU21_07280"
     CDS             complement(2643..2786)
                     /locus_tag="VMU21_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77366.1"
                     /translation="MKNESFYLLPIPKEHDIIAGSTTGIIEGNEAEGLPFWFSRKSKK
                     GEK"
     gene            complement(3054..3725)
                     /locus_tag="VMU21_07285"
     CDS             complement(3054..3725)
                     /locus_tag="VMU21_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77367.1"
                     /translation="MNPEESNCLDEVGTFLKQINVNLPKKISAASLSHHSKLLFKAFS
                     LRGVLVHRITDLTEVAIDLYEADKVVPAIIMTRSVFETTAVLYLLYKKIKKAAMTKML
                     GDIDDFLMKSLFGGRVKNAPIESYNILTAINHADKDFSSYRESYDRLSEFVHPNWSGV
                     SGAYSKLNRSTMELYLGKEFGKVPIMISLPLLAGALELFIHYYDELDHYLLKFNKFCD
                     QLHRK"
     gene            complement(3722..4327)
                     /locus_tag="VMU21_07290"
     CDS             complement(3722..4327)
                     /locus_tag="VMU21_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO77368.1"
                     /translation="MADILLGLEEYKTLVEQAPIMIWRSDTKASCDYFNERWLAFTGR
                     SMEQEYGNGWAEGVHLEDFQRCLKIYLEAFKKREIFEMQYRLRRHDGVYRWIFDRGVP
                     FFDPRGDFAGYIGSCVDVTEKVEAQEALHRAQMAEMNQLRGLLPICTSCKQIRDDKGY
                     WNQIEVYIREHSEAEFTHSICPKCAKELYPEIYAKLYPDRS"
     gene            complement(4500..4811)
                     /locus_tag="VMU21_07295"
     CDS             complement(4500..4811)
                     /locus_tag="VMU21_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77369.1"
                     /translation="MKKVIGIVVFALLVFVFASTFSEIGAREAVAQTTGKKSSAKLLM
                     RVSGQVTAVDREKNSLTLEGITIVADEAMLANIKEGDTVTVDYYTRDVHRAISIVPEV
                     K"
     gene            complement(4897..6336)
                     /locus_tag="VMU21_07300"
     CDS             complement(4897..6336)
                     /locus_tag="VMU21_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUO77370.1"
                     /translation="MKPVKPFSFAVAVTFVVLFLGHGMLPDLIIFRNACAETVKIPQE
                     AIDALSKTGQAMAEIAAAARPTIVNVASTDTTKIRETQDPSSQNEYGQPEKPKERKQL
                     SLGSGVIVDENGYILTNKHIIENPDSIKVRLFDKREFKGLLIGADPKTDLAVIKIDAK
                     HLPVLKIGDSDRLRVGETVMAIGNPYGLSQTVTSGIVSATGRADVGIAEYEDFIQTDA
                     AINPGNSGGALVNMRGELIGINTAIFSTTGGHQGIGFAIPSNMAKVVMKSLMLNGKVI
                     RGWFGASIQALTPELARIFNLKDERGALVADVFEGSPAEKAGLKRGDVIIELNNKEID
                     EPLNLRNMVANTLPKTEVTLKIIRKGKVDVVRGTIEELPTETVKSSTAVDNYLKGIYV
                     QNLTPELKKTFDIPKRIAGVAIIDIEDGTPADGVLMKGDVIMEVNNKLINNVKDFGTS
                     VSEIMPKQTVLLLIYRSDSTTYITLSGEQ"
     gene            6582..6791
                     /locus_tag="VMU21_07305"
     CDS             6582..6791
                     /locus_tag="VMU21_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUO77371.1"
                     /translation="MGGLFQPMHLLVILVIALVIFGPGKLPELGSSIGKGIKEFKKAM
                     TGDEKDAVTEIKKEVTQQDSRDGKA"
     gene            complement(7216..8433)
                     /gene="glp"
                     /locus_tag="VMU21_07310"
     CDS             complement(7216..8433)
                     /gene="glp"
                     /locus_tag="VMU21_07310"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HUO77372.1"
                     /translation="MEEAQKRIAAHLIAKEPPRIALPIEESYKRVLAEDIFSPEDLPS
                     FSRSTVDGFAVLSSDTFGATEGLPAYLNVTNEISMGEEPQFILKKGGAAKIPTGGMLP
                     QGSDAVVMLEHAQCADEGLMEVFRPVAPGENVISAGEDVRKGERILTKGRMLRPQDVA
                     ALAGIGITDVMVYEKPKVSIISTGDEIVPAKRALRPGQVRDINSYNLAGLITEHGGLA
                     VKKGIVKDVFDGIQRVVKESLDTCQMVIITGGSSVGIMDMTSKVIDSLGSPGVIFHGV
                     TLKPGKPTIGGVVNGIPVFGLPGHPAAVTVCFDLFIKPVLRILTGVHEKEHMKKKRVL
                     TARIAKNVSSTPGREEHVRVVLEPRGDEVWAVPVLGKSGLIRTLVMADGTVVIPAQVR
                     GLQEGDTVEVLLF"
     gene            complement(8536..9285)
                     /locus_tag="VMU21_07315"
     CDS             complement(8536..9285)
                     /locus_tag="VMU21_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HUO77373.1"
                     /translation="MKTFKLLVVVSFLLLPSLAFAWGPLTHIYLGSEIVSLGSLLPAG
                     VYALIRKYRHDFLYGNLMADIIIGKKFLPEDKNPHSWDVALSLLDSAKTQQQKAFVYG
                     YMSHLAADTVAHGSFTNTKKNIGHTLVELRADSMIDKRYWLQAVRIDRRVQMRNDSFL
                     ENSLERALFSFKTNKRIFKGMLLLSCFNKERVGEFIQKNAVNPSVLTRGNILQLHTES
                     LDRMVDILCNGKSSEVINESPMVCVRYRKAS"
     gene            complement(9282..10742)
                     /locus_tag="VMU21_07320"
     CDS             complement(9282..10742)
                     /locus_tag="VMU21_07320"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HUO77374.1"
                     /translation="MKIIIRDLKEKECMCDALILPLHEGEGVTAYREIESALNGLLSK
                     VISSREFLGKHTQTSLIHTMNRIGPRRILLIGLGKREEVSAEKLRQAGGKALSYLHGL
                     GLKEVAFSPKHIPSVKHSPMPFVEGGLLSLYQFKKYKKEDDTTKGVEKFTLIGKPGPS
                     LKESLRWTVASSSAVNFARDLINAPSNDMTPTMLAKTARSLAGGRVAVKVFEKRDAER
                     LGMGAYLSVARGSMEPPKFIVVKYNGGKGAPVALIGKSITFDSGGISIKPSEGMEKMK
                     YDMAGGAVVLGVLKAASEMGLPVNLVGILPATENLPGGTATKPGDIVTSLSGKTIEIV
                     NTDAEGRMVLADGIGYVKKFKPKVIIDIATLTGACGVALGNEAIAMMGNDSKVMEKLK
                     TIGDEVYERVWQMPLYEEYLEYIRSDVAELKNSGGKTGSLVTAAYFLKEFAGDTPWVH
                     LDIAGTAWTEKDKPYVPKGATGIGVRLLLNLLKEMK"
     gene            10886..11482
                     /locus_tag="VMU21_07325"
     CDS             10886..11482
                     /locus_tag="VMU21_07325"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HUO77375.1"
                     /translation="MATKKSKVTQKKSLKPAKKKITKKVKKTPTNAKVTKKVAPKVPK
                     AKTKGKKTEKRPAVRAVKKILASKNKKISPSKKLTPREKLLQNVKQTLMMQRNILLNE
                     AEEALNLLPGQTIFPDMGDQASAEIDRSFMLRLRGREQRLLKKIEAAIDKIDNGTFGI
                     CEACGEEINIKRLEARPVTNMCIFCKTEQEEEEKLRGA"
     gene            complement(11490..12284)
                     /locus_tag="VMU21_07330"
     CDS             complement(11490..12284)
                     /locus_tag="VMU21_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO77376.1"
                     /translation="MIKIILSALIVIILSSILAPLISPFNPEEINLDELRQSPSLRHP
                     LGTDNKGRDILSRVLYGGRISVSVAIMAAALSMGIGLIVGLISGYIGGKIDTSIMLAV
                     DLILAFPSLLLAIGISVILPPGIYTVMIAIASVGWASFARLIRGHVLSCREAPFIEAA
                     QAMGCSHRRILLVHLLPQCLPLVVVMMGLRLGGYILTEASLSFLGLGAQPPMPTWGSM
                     VSAYRVFIVSAPWMVIFPGLAIAVTSLCFNMLGDALRDKYGLRVAG"
     gene            complement(12277..13209)
                     /locus_tag="VMU21_07335"
     CDS             complement(12277..13209)
                     /locus_tag="VMU21_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO77377.1"
                     /translation="MKFYIIKRFFLLIPLLLGITILTFSLTKALPGDPVFSLVGERAS
                     PEVIESIRRELQTDRSFFVQYFGYVKLLLKGEFGRSYYTNRKVLDDLLDKFPNTLLLA
                     LGAMLIAVPAGISLGFLSAVRRGTAVDKGISSLSVIGLSLPVFWSGLLIMLFLSLKLR
                     LFPPSGMGDWRFLIMPAITLSLPALATLTRVSRTTLMDLLGMPYVRTARAMGIKEYKV
                     KGVHIFKNALIPLVTVVGLDFGSYLNGAVLTETIFGWDGIGRFAMEGIIKRDYPVIMG
                     CIIVGTVVYVLINLCVDILYHYLDPRVRLYEEND"
     gene            13350..14309
                     /locus_tag="VMU21_07340"
     CDS             13350..14309
                     /locus_tag="VMU21_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HUO77378.1"
                     /translation="MKAVLIRQFGPPGVMKIEEVEKPVISRDRLLVRIFYSSVNPVDW
                     KIRNGSLRLISGSRFPMMLGFDIAGEVVDTGSAVTRFKKGDRVFGMLDFRHRGAYAEY
                     ACANEAGLALIPETLDFKEAAAIPLAALTAYQALHYKGRIKNGKTVLINGASGGVGSF
                     AVQIAKASGAAVTAICGTHTTELVKKLGADSVIDYTKHDFRQFSDTYDIIFDVVAKHS
                     FFEVSKNLRGGGYYVTTLPNKPADILSFVLTFPLSFLGLSKRSTFISVRPNGDDLHSL
                     SLLVKEGKIMPLIDREYTLEEINQAHAYSEKGHARGKILIRVS"
     gene            14323..14955
                     /locus_tag="VMU21_07345"
     CDS             14323..14955
                     /locus_tag="VMU21_07345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO77379.1"
                     /translation="MITYFLNALKRLAQKAIIKGAVQKITELSPFISLLKRRKLNTLV
                     EIGTARGGTLYAWCKIAEPDAQIVSIDMPGDPFGGGYSARDIRKFKTYKKENQKMYFM
                     RKDSHRNATKNELIRILNGRKIDFLFIDGDHRYSGVKKDWELYSPLVKQNGLIVLHDI
                     LAHPKVPQCKVDRLWKELKLHYTHREFLDRRDDRGWGQWGGIGVLYYVQH"
     gene            complement(14980..15864)
                     /locus_tag="VMU21_07350"
     CDS             complement(14980..15864)
                     /locus_tag="VMU21_07350"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HUO77380.1"
                     /translation="MGAEAVVLKTEMPDIGMPKRGKVRDIYDLGEHLLLIASDRISAF
                     DVVLPNGIPGKGKVLTQISIFWFRQTEDIVRNHLVATDVDEFPPELRKYRDMIEGRSM
                     LVKKARPMAVECIVRGYLSGSGWKEYQEKGTICGIRIPHGLVESSRLDQPIFTPSTKA
                     EEGHDINISFEETEKIVGKESAEKLKQTSLRIYSKARDIAEKKGIIIADTKFEFGMHH
                     NELMLIDEVLTPDSSRFWSAKGYKPGKGQDSYDKQIVRDYLLTLDWDKTSPGPVLPPD
                     IVSKTVERYREIMKILTK"
     gene            15918..16427
                     /gene="def"
                     /locus_tag="VMU21_07355"
     CDS             15918..16427
                     /gene="def"
                     /locus_tag="VMU21_07355"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUO77381.1"
                     /translation="MALLEIKTYPEKVLKQKASLVEKIDGKLQRLIDDMVETMYAAPG
                     IGLAAPQVGISKQLMVIDVSLREEEKRPLIVLINPEIKHVEGEMESEEGCLSLPGYVT
                     QVKRSERIEVCGLDRTGKPVKIEGEGLLSRALQHEIDHLNGTLLIDRISRIKREFFKK
                     KFNKALSKV"
     gene            16629..17549
                     /gene="fmt"
                     /locus_tag="VMU21_07360"
     CDS             16629..17549
                     /gene="fmt"
                     /locus_tag="VMU21_07360"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HUO77382.1"
                     /translation="MSIIFFGTPEFALPSFWSLINSGEEISLVVTQTDKLKGRGHKLS
                     PPPVKTAALEAGIRVAQPGTLRDDKFIEALRFLKPEFIVVIAYGKILPDAILETPEHG
                     CLNVHASLLPNYRGAAPIAWAILRGDERTGITTMLMDEGLDTGPILLQQETDISRDDT
                     AGSLSVKLARLGASLLIETLKGIRDGSVKPRVQSGEASYAPPLRKEDGLIDWSRSAVE
                     ISNFVRGMQPWPSAYCTLEGARVTILKAEPLEGSGLSGTVIRSSKGELVIGTGKGRIS
                     LLELQPSGKRPMPASAFLLGRKLMEGMVLK"
     gene            17558..18067
                     /locus_tag="VMU21_07365"
     CDS             17558..18067
                     /locus_tag="VMU21_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HUO77383.1"
                     /translation="MRGLTLKVLFPLFMLLGSFSAEKKERLQGLIISLNNKLLGMERP
                     RAKRILLLLPHCLQINECDIRLTYNIYNCKRCGKCEIKDLIQIADDNNLTLFIATGGN
                     LARKIVKDVLPEAIVAVACERDLSSGIADSYPLPVLGIPNERPFGPCVNTRVNLEKVK
                     GAIEFFCKK"
     gene            18082..>19027
                     /locus_tag="VMU21_07370"
     CDS             18082..>19027
                     /locus_tag="VMU21_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HUO77384.1"
                     /translation="MPVIKSMPTTRALALAALRKICEQGKKPKGMIEELSGSLDKRER
                     SFLMELVYGVLRYRDTLDWALHGFLRKPLGLSQDTRNNLRLAAYQILFMRVQDWAAVN
                     EAVELEKPKGRPEVVNGVLRSLLRKPATERINLTPMERKGAVPYIALVTSHPSWLIKR
                     WVKRFGEKGARELAEANNRIPPLTLRANTLRSTREQLLERLSAMGITGEPTPCSPEGI
                     RLTGFPVIKELSSLRDSFIVQDEAAQLITYLLEPRPGERILDACASPGGKTTHIAQMM
                     EDRGEILALEIDEARMPQLKENVSNLNMNSITLLHRDFR"
BASE COUNT         5019 a         4639 c         4438 g         4931 t
ORIGIN      
        1 cctgaaaaga ttataacgta actgcaggtg cttttggtat gtttggcggt caagtggaga
       61 tggtaaagct catcaaggac ctgaagctga aagtgaatgc agagatacaa aaggaccagg
      121 tcagggtaag ggcgaaaaaa aatagacgat ctccagacga ttatggccaa actgaaggaa
      181 gaagacttcg ggatacatct ccagtccacg aactacagat agtcgctgcg aaacaaagtc
      241 ttttctcccc ccttcccttt ttgatcagcg tttaaacatt ttgtatatct ttgtcgtcta
      301 atgtttagag gcataagcga acagatagag gcttgctcaa gagatatagt agctatctca
      361 aaagacgtta tagattgctc aaatacatag gaggtcactt atgaaaagaa ggattctcag
      421 aatattcatt atttgtgtaa ctatgtccca tttaggatca tcaataagca ccgctgcaga
      481 tgtaaaggtg atgtacgcca ttggactttt ggactacggt gaaaatgcga ggcttgtgac
      541 aactgaggaa tttaatcaga ccgtggcaaa ctacgaaaaa tttagagatc gcgttgcaaa
      601 ggccccaacc ttgaaagcga tggtacagaa tggatggacc attgtgcatg tggagcagat
      661 tcgggcagga gatggggatc cgtatagaga gctgaattac ctaatcattt ttcaaggagg
      721 taggcgttga taatgccttc tgtccctaac gcctgagtgt cggcgcggga agctaccttt
      781 gccacaaaat ggggtgttta cgccgcagca gctggatttt atggttaggt tgtctttatg
      841 ccccacgctg accctgcaaa atataatctt atataagtcg ttcatcaagt gcatattccg
      901 gcgtttgagg aaaactaaca aaaaaaggcc tggttacccg ggcccttttt ctaaatgtcc
      961 aaagggcaag cctgaggatt atttcgccgg tccttcactg tcttgccagc cctcaccaaa
     1021 gataaacttt ttaccgtcca tctccacttc gatacgtccg gagccggtgc gttggagctc
     1081 gacagagggc gggggttgcc aggggctatc gaaaataaag ctgcccttcg gaatcagccg
     1141 tatcacctta tagtcttcgt cgatgtaata attatagctt gcgaagaaag aagaacccaa
     1201 aaggttttca ctggatgaag ggacataggc aacgtcataa ttatatcctc ggatacttcc
     1261 gatcacaatg gaattaagcg tgaggacata gccctcaaca atgccgccgg cagtgtgtgc
     1321 ctgaatcttc ctggcgtcgc tgaactctat gatgccgagg ctctgcagtg cagcactgtt
     1381 aagaacaatg ctattagacc cggtatcgaa gacgagttcc acaggcactc catttatttg
     1441 cacggtgaca tacttcagac tgccccgctc agtaaaagga atggcttcat cagccaggac
     1501 gttctcgccg atgatcaacg cgggcaataa agaggaaagg aaaaagaaca cggtcacgga
     1561 aattcttgcg atcacaacgt cctccctatt tgaaacctgt acgctatttg tattttacca
     1621 tgttcggagc aaagtaacaa aacgaaagaa agcctatttg agtctctctt atcataccgc
     1681 tatgactacc ttccaaagga gttgcgctat cctcatcaga ttagatgctg ttccaaccaa
     1741 tagggatgat ggtggccaat atcggcacgc cagttgttgt gttacgctcc gtactcttct
     1801 ggcgataaga catgaaatat gcgccctttg aggatgacta tgaagtgttt aattcaggaa
     1861 gggcatctcc gtgcatgttt cggggcgaaa gcgcactaag cctgaccgca ctgtacgttt
     1921 aacaggttgg tgcgtttgaa agctacggat tcgaaatcgt taccggaacg gtaaccgaac
     1981 tagcattatc tgtaaccttc aaattgacaa ttgtgccatt tgccccggcg cacgcagtgg
     2041 ctgttactgt aaattggcca ttggtcgtta gagtatcggt agaaatagta acgagactcc
     2101 catttgaaga tgcggttaca ctgtaagatg caggagttcc gcccgttacg agaaatgacg
     2161 cggtttgtcc taatgaacaa cttaacgtag ccgctgaggg tgttacggaa atcccgctag
     2221 gagcaatctc cctattggcc tgccaagtgc cagacccgtc cgaacctgtg ctcgtgtatg
     2281 tccctgagag cgtgttccaa tctttgcttg cgctaccagg taaggttgtc gtagtcccat
     2341 ctgagttatt aagagtaata acgaaattgt tcccgtcgaa agtaccagta agtgtggcgt
     2401 tatccgatgt ggttccggaa aagggatcga atcttttgtc ctgtgtgata gaaagctttg
     2461 tctcgcgtac agacacgttg gactgggtaa gggacatatc ccaggttccg ctcactttgc
     2521 cttggtttat atcctcgtag aatgtatacc cgccgcaccc atggagcaat aaagataaga
     2581 tcacaaccaa ctccggccaa ttcattgtgc gataccgttt cattagtctc cgttcgaatt
     2641 cgtcacttct cccctttttt gcttttcctt gagaaccaga atggcaagcc ctcagcctcg
     2701 ttgccttcaa taattccggt tgtactaccc gcaattatat catgttcctt aggtatgggc
     2761 aacaggtaga acgactcatt tttcatctgc ctctttgagg tgtagataat tagcagttat
     2821 caagagtgca tgagcaaaac agaatatcta tagttataag aagcgtttac aacaactgta
     2881 aacaaaaata tttcgtgcca catcttatgg gtaaaaataa tgagcaaggt ctttatcttg
     2941 atcccttgct caagctcaca accttaaaac atggtgttac gtacagataa gagataacgg
     3001 ctaaactgtg cagcgcagtg aaatgaagtc cgcacgagtg atacgttggg tcactatttt
     3061 ctgtgcaatt ggtcacagaa tttattaaac ttgaggaggt aatgatctaa ttcatcgtaa
     3121 tagtgaataa atagttcaag tgcgccagct agtagcggaa gagaaatcat gattggcact
     3181 ttgccaaatt ccttgcctaa atagagttcc attgtgcttc gattcagttt gctatatgcc
     3241 ccactgacac ctgaccagtt agggtgcaca aattcagaga gcctatcata cgattctcta
     3301 taagagctaa agtctttatc tgcgtgatta atagcagtaa gaatattata gctttcaatt
     3361 ggagcgttct tgactctacc gccaaataaa gatttcatta ggaagtcatc aatatctcca
     3421 agcatcttcg tcatcgcagc tttctttatt tttttgtata gcaaatataa gacagcagtt
     3481 gtttcgaata cagagcgtgt cattattatt gccggaacta ctttgtcggc ttcgtaaagg
     3541 tcaatggcga cctcagtcag gtcggtaatc ctgtgtacaa gaacccctct taatgaaaac
     3601 gctttaaata gtaacttaga gtgatgactc aatgaagctg cagatatttt tttaggaagg
     3661 ttgacattta tttgcttcag aaatgtacca acctcatcta agcaatttga ttcttcggga
     3721 ttcaacttcg atccgggtat agctttgcat agatttccgg gtacagttcc ttcgcacatt
     3781 tggggcagat actgtgagtg aactctgctt cggagtgctc cctgatgtac acttcaatct
     3841 gattccaata gcccttgtca tcgcggattt gtttacacga cgtgcagatc ggtaaaagac
     3901 ctctcaattg gttcatctcg gccatctgcg ctctgtgtag cgcctcctga gcttctacct
     3961 tctcggtcac gtcgacacaa ctcccaatgt aaccggcgaa gtcgccccgc ggatcaaaaa
     4021 agggcacgcc acgatcaaat atccaccgat acacgccgtc gtgcctacga aggcggtact
     4081 gcatctcaaa tatttctcgt tttttaaagg cttccaaata aatcttcagg cacctctgaa
     4141 agtcctcaag gtggacccct tccgcccacc cgttaccgta ctcttgctcc atcgagcgcc
     4201 cagtgaatgc cagccacctt tcattaaaat agtcgcatga ggcctttgta tccgagcgcc
     4261 atatcatgat tggagcttgc tcaacaaggg ttttgtattc ttcaagaccg agaaggatgt
     4321 ctgccatatc tgcacctccg ctagctgtgt gataaaacat acaataaatt actgccaata
     4381 tgagaagcgt ccaaaaattt attaagcgaa caacttcagt ctatcagact tcaatgcact
     4441 tgcaaagtaa agttgacgta agtataaagc gatactaaaa caagtttctt gaacttaact
     4501 catttaactt ctggcactat ggagatcgct ctatgcacat ctcttgtgta gtaatctacc
     4561 gtcaccgtgt cgccctcttt tatgttggcg agcatcgcct catctgccac tattgtaata
     4621 ccctctaacg ttagagagtt cttttctcta tcaactgcgg ttacctggcc tgaaactcgc
     4681 attaaaagct tcgcactgct cttcttgcca gtggtctggg cgactgcctc acgtgctcct
     4741 atttcggaga acgtcgaggc aaagacaaat accaatagtg cgaatacaac aatccctatt
     4801 accttcttca taacacacac ctccttagat ctacgtatca ttcaccagcc ctcatagatt
     4861 gcatctgctt taattcacta gttttaggaa gacctctcat tgttctcctg agagtgttat
     4921 atacgttgtg gaatcgcttc tgtatatcag caggagcaca gtctgttttg gcataatttc
     4981 tgaaactgaa gtgccaaagt ctttcacatt atttatcagt ttgttattca cctccataat
     5041 gacatcacct ttcattaaaa ctccatcagc aggggtgcca tcttctatgt cgattatggc
     5101 cacaccggca atgcgtttag gtatgtcgaa ggtctttttc aattcaggag taaggttctg
     5161 gacatagata cccttgagat aattatcaac tgcagttgat gactttactg tctctgtagg
     5221 cagttcctca atagtacccc ttaccacatc tactttccct ttacgtataa tttttaaagt
     5281 cacctcagtt tttggcaatg tattggccac catatttctg aggtttaatg gctcgtcaat
     5341 ctccttgttg ttaagctcta tgatgacatc ccctcttttt aaaccggctt tttcagcggg
     5401 acttccttca aatacatccg caacaagggc tcctctctca tctttcaagt tgaatattct
     5461 tgcaagttcc ggggttaacg cctgtattga tgcgccaaac cagcctctga tgaccttccc
     5521 atttaacatg agacttttca taaccacttt tgccatattg cttggtatgg caaagccaat
     5581 cccctgatgt ccgcccgtag tgctgaatat ggcggtattg atgccaatga gttctcctct
     5641 catgttcacc agggcgccgc ctgagttgcc ggggttgatc gcagcatcag tctggataaa
     5701 atcctcatat tccgcaatac cgacgtccgc ccttcctgtt gcgctcacaa tacccgatgt
     5761 aaccgtctga cttaatccat aaggattccc gatcgccata actgtttcgc ccactctcag
     5821 tctgtcagaa tcacctattt tcaaaaccgg aagatgtttg gcgtctatct taataaccgc
     5881 gaggtctgtt tttggatcag caccgattaa gaggcccttg aactctcttt tgtcaaaaag
     5941 ccgcaccttt atactgtctg gattctcgat aatatgttta ttggtgagta tatagccgtt
     6001 ctcgtccacg ataacgcctg agccaaggct cagttgtttg cgttcctttg gtttttcagg
     6061 ttgcccatac tcattttggg aggaagggtc ttgcgtctcc cttatttttg tagtatctgt
     6121 cgatgccaca tttacaatag tgggtcttgc tgcagcggca atttctgcca ttgcctggcc
     6181 tgttttcgag agtgcatcaa tggcctcttg cggtatcttg accgtttctg cacacgcatt
     6241 acggaaaatg atgaggtctg gcagcatacc atgcccaaga aacaaaacaa caaaagttac
     6301 ggctacggca aaagaaaatg gttttacagg tttcaagtgt ttcctcctgg tgagttttgg
     6361 ccagagtacc aagcagcaca ttttagcgcc tctgacaaat ctcttcttgt tctttcctgc
     6421 ttttcttatt ataaattata cctttcttat tataaccttc aactctatag attaatgcaa
     6481 ataccgccct gtgcttattc catggatctt tcataagtat agagaaacaa tatgacaatg
     6541 tgctaaagta taaaatatat acttaaaggg aggtgaaaga catgggagga ttgtttcagc
     6601 caatgcatct gctggtaatc ttggtgattg cgctggtcat ctttggcccc gggaaattgc
     6661 cggagttggg ctcaagtatt gggaagggca taaaagaatt taagaaggct atgactggtg
     6721 atgaaaagga tgcagttaca gagatcaaga aagaggtgac ccagcaggat tcccgggacg
     6781 ggaaggctta aatagaataa ccattgaaga gagattaacc aaccatatcg cggtggattt
     6841 cttcaagcca tccaaaccac tttaactctc agcaagtcct tcgttagata aaacggcggt
     6901 cctcccatgc tggtgtttgt aaatactacc aatgctacgt gtctgccgtg aaaatccgga
     6961 aggtaaatgg ccattgttgt gaatcctggc ccatcgccgc cgtgacagaa aaatcccccg
     7021 tatccccaat ccggatcgat cataagacct aaaccgtacc cagggttacg aaaaaatgtt
     7081 ggctgaggcg aagtatgctg tccggcgttt actcccctgg aataagaatg gaaaatagtt
     7141 agggcataac aaacgacctc aaccgtatgg cgactacgtc ttatggagaa tagattgtaa
     7201 gtgattagtg tttgactaga agagcaggac ttccacggta tcgccctcct gcagtcccct
     7261 tacctgcgca ggaattacca cagtgccatc ggccatgaca agggtcctta tgagccctga
     7321 tttgcccagc accggaactg cccatacctc gtcccctctt ggttccagga caacccttac
     7381 atgctcttct cttcctggag ttgaggacac attctttgcg atacgagcgg ttagtaccct
     7441 cttctttttc atatgttcct tctcgtgaac accggtgagg attctgagaa caggctttat
     7501 gaagaggtca aaacagacag tgactgctgc gggatggccg ggaagcccga acactggaat
     7561 accattcacc accccgccaa tggtaggctt ccccggtttg agcgtaacac cgtgaaagat
     7621 gacccctggt gaaccaaggc tgtcaataac ctttgaggtc atgtccatga tacccacaga
     7681 actcccccct gtgatgatga ccatctggca tgtatccaga gactccttta ccacacgctg
     7741 tatcccgtcg aaaacatctt tcactatgcc ctttttcact gccagaccgc catgttcagt
     7801 gataagaccg gcaagattgt atgagttgat atcccggacc tgacctggtc tgagggctct
     7861 ctttgcaggg acgatctcat ctccggtgga gatgatcgag accttcggtt tttcatacac
     7921 catcacatca gtaatcccta tgcctgcaag ggccgccaca tcctgaggcc tgagcatacg
     7981 tccttttgtc aatatccttt cgccctttcg tacatcctca ccggcagaaa ttacattttc
     8041 accaggggcc actggtctga atacctccat cagtccctca tctgcgcact gggcatgctc
     8101 cagcatcacc actgcgtcac tgccttgagg gagcatcccc cctgtgggga tctttgccgc
     8161 ccctcccttt tttagaatga attgaggctc ctcgcccatc gaaatttcgt tcgtgacatt
     8221 caggtatgcg ggaagaccct cggtcgcacc gaaggtgtcg cttgagagta ctgcaaatcc
     8281 gtctaccgta gaacgtgaaa aggagggcag gtcttcaggg gaaaagatat cttctgcgag
     8341 aaccctcttg taggattcct ctattggcag tgcaatccgc ggaggttctt tggcaatgag
     8401 gtgcgctgct atgcgtttct gcgcttcctc cacagaaatc gcatcctctc ttccaagcat
     8461 gtcacgcata gattaactcg ggggtataat caggaatccg gaatgaagcc tctccgcgcg
     8521 ggggtcaccc accactcatg aggccttgcg gtatctgacg cagaccatgg gactctcatt
     8581 aatcacttca gaagatttcc cattgcacaa aatatccacc atgcggtcca gggactcggt
     8641 gtggagctgg aggatgttgc ctctggtgag cacagaggga ttgacggcat ttttctggat
     8701 aaattcgccc acccgctcct tgttaaagca ggagagcagg agcattccct tgaagatcct
     8761 cttgtttgtc ttgaaagaga acaaggcgcg ttcaagcgag ttttccagga agctgtcgtt
     8821 cctcatctgg accctcctgt ctattcttac tgcctggagc cagtatctct tgtcgatcat
     8881 actgtcagcc ctgagctcca caagggtatg ccctatgttc ttcttggtgt tggtgaaact
     8941 tccgtgggcc acggtatccg ctgcgaggtg gctcatatac ccgtagacga atgccttctg
     9001 ctgctgggtc ttcgcagaat cgagaaggct taacgccacg tcccagctat gggggttttt
     9061 gtcttcaggg agaaattttt ttccgatgat aatatccgcc atgagatttc cgtaaaggaa
     9121 atcgtgcctg tatttcctta tcagggcata cacccctgca ggcagcaaag aaccaagaga
     9181 gactatctcg ctgccgagat agatgtgggt gagaggtccc cacgcaaagg ccagcgacgg
     9241 taataagaga aaagagacga caaccagtaa cttaaatgtc ttcatttcat ctcctttaat
     9301 aaattcagca ggagcctgac gccgatacct gtagcaccct tgggaacata aggtttgtct
     9361 ttttctgtcc aggctgtgcc ggcgatgtca agatgcaccc acggggtgtc tccggcaaac
     9421 tccttcagga aatatgctgc ggtgacgaga gaccctgtct tccctccgga attcttgagt
     9481 tccgcaacat cacttcttat atactcgagg tattcttcat acaggggcat ctgccacacc
     9541 cgctcgtaca cctcatcacc aatcgtcttt aacttctcca tcactttgct gtcatttccc
     9601 atcattgcta ttgcttcgtt acccagggca accccgcatg cgcctgtcag ggtcgcaatg
     9661 tctattataa cctttggctt aaattttttc acgtatccga taccgtccgc gaggaccatc
     9721 ctcccttcgg catcggtatt cacgatttca atggttttgc ctgacaaaga ggtgacgata
     9781 tcacccggtt ttgtcgctgt ccctccggga agattttctg ttgcgggaag aatgcccaca
     9841 agattcaccg gaagccccat ctcagaggcg gcctttagca cgccaagcac cactgcccct
     9901 cctgccatgt catacttcat cttctccatc ccttcgctgg gctttatcga aatacccccg
     9961 ctgtcaaagg taatggattt tccgataagg gcgactggtg cccccttacc tccgttgtat
    10021 ttcaccacaa tgaatttagg gggttccatg gagccccttg ccacagagag gtaggctccc
    10081 attcccaggc gctctgcgtc tctcttttcg aagaccttta ccgcgaccct gccacccgca
    10141 agagacctgg ctgtcttcgc gagcatggtg ggcgtcatgt cgtttgaagg agcattaatg
    10201 aggtcccgtg caaaatttac cgcagaagac gaggcgactg tccagcgcaa agactcttta
    10261 agcgacggcc caggttttcc tatgagcgtg aacttctcta ccccttttgt ggtatcgtct
    10321 tcctttttat acttcttgaa ttgataaagg gagaggagcc caccttcgac aaaaggcatg
    10381 ggtgagtgtt tgacagaggg aatgtgtttt ggagaaaatg caacctcttt cagcccgagc
    10441 ccatggagat atgaaagggc ctttccccct gcctgtctca gcttttcagc actcacttct
    10501 tccctcttcc ccagaccaat gagcagaatt ctgcgtggtc ctattctgtt cattgtatga
    10561 atgaggctgg tttgagtatg tttccccaga aactcccggg aggatatgac cttgctgaga
    10621 agaccgttca aagcggactc gatctcacga tacgcggtga ccccttctcc ctcatgcaag
    10681 ggtaaaataa gggcatcgca catgcactct ttttccttca ggtcccttat gataattttc
    10741 atagtgacgt aatgcctgat ttaccactgc aagaacaaat atgcatattt ttttatttta
    10801 tcataaatag attttattat cttctgtggt tgactaacat ttacggtttg tggtaatttt
    10861 gaaaattacg ttctgaggag aagacatggc aacaaagaaa tccaaggtga ctcaaaagaa
    10921 gtccctgaaa cctgcgaaaa agaaaatcac aaaaaaagtg aaaaaaacgc ctacaaacgc
    10981 caaagtcaca aaaaaggttg ccccaaaggt gcctaaggcg aaaacaaagg ggaaaaagac
    11041 agagaagaga cctgcagtta gggcggtgaa aaagatactt gcctccaaga acaagaaaat
    11101 ctctccaagc aagaaactca cgccaaggga aaaactgctt caaaacgtaa agcagaccct
    11161 catgatgcaa cgcaacatcc tgctcaacga ggcagaagaa gcgctcaatc tgttgcctgg
    11221 ccagaccatc ttccctgata tgggtgatca ggcaagcgcg gagattgacc ggagcttcat
    11281 gctgcgcctg aggggaagag agcagagact gctcaaaaag atagaggcgg ccattgacaa
    11341 gatcgacaat ggaacgttcg gtatctgcga ggcgtgtgga gaagagataa acatcaagag
    11401 gcttgaggcg cggcctgtga ccaatatgtg catcttctgc aagaccgagc aggaggaaga
    11461 ggaaaaactg aggggagcct aacccttccc tatcccgcta cccttaaccc atacttatct
    11521 ctcagtgcat ctcccagcat attgaagcag agggaggtca ctgcaatggc aagccccgga
    11581 aagatcacca tccaaggcgc tgagacgatg aataccctgt acgcactcac catggaaccc
    11641 caggtcggca tagggggctg ggcgcccagc ccgagaaagc tcaatgacgc ctcggtgagg
    11701 atataccctc caagccgcaa tcccatcatc accaccacga ggggaagaca ttggggaagg
    11761 agatgtacca ggagtattct tctgtggctg caacccattg cctgtgccgc ctcgataaag
    11821 ggcgcctccc tgcacgataa cacatgcccc ctaatgagcc tggcaaagga agcccatccc
    11881 accgaagcaa tagcgatcat gactgtatag atgccgggag gaaggatcac cgatatgccg
    11941 atggcgagaa ggagggaagg aaaggcgagg ataagatcta cggcgagcat tatcgaggta
    12001 tcaatctttc ccccaatata gcccgagatt aagcctacaa taaggccgat acccatggat
    12061 aaggcagccg ccattatggc cacagaaacg gagatcctcc cgccgtaaag gaccctggaa
    12121 agaatatccc ttcccttgtt gtccgttcct aagggatgac gcagactggg agattgtctc
    12181 agctcatcaa ggtttatctc ttcaggatta aagggtgata tgagaggcgc caggatcgac
    12241 gaaaggatta tcacaatgag tgcagaaaga ataatcttaa tcattttcct cgtagagcct
    12301 caccctggga tcgagataat ggtagagaat gtccacacaa agattaatca ggacatacac
    12361 aactgtaccc actataatgc atcccatgat tacgggataa tcccgtttta ttatcccttc
    12421 catggcaaac cttccgatac cgtcccatcc gaatatggtc tccgtaagca ctgcgccgtt
    12481 caggtaactc ccgaaatcca gtcccacaac cgtcacgaga gggataaggg cattcttgaa
    12541 gatatggaca cccttgacct tgtattcctt aatccccatt gccctcgccg tcctcacata
    12601 cggcatgccc agcaaatcca tgagggtagt tctcgaaacc ctggtcagtg tggcaagggc
    12661 gggaagggaa agggtaatcg ctggcatgat gaggaatctc cagtccccca tacctgacgg
    12721 aggaaatagt ctgagtttca gactgagaaa gagcataatc aggagtcctg accaaaaaac
    12781 cggaagactc aaccctatta ctgacagaga agagatgccc ttgtcaacag ccgttcccct
    12841 tctcactgcg gatagaaagc cgaggcttat accggcaggc accgcgataa gcatcgcacc
    12901 caatgcgagg agcaatgtat tggggaattt gtctaacagg tcatccagaa ctttcctgtt
    12961 cgtgtaatac gaacggccga actccccctt cagaagcaat ttaacgtaac cgaaatactg
    13021 aacaaagaag ctcctgtctg tctgcaattc ccgtcgtatg ctctctatca cctcagggct
    13081 tgccctttct cccacaaggc tgaacacggg atcaccagga agcgcctttg tgagggagaa
    13141 ggtaagaatg gtgataccta acaggagagg tatcaggaga aaaaatcttt tgatgatgta
    13201 aaacttaatg gtttcctcct gtaatgagag ttcttaaagg atacccctgg attctaaaca
    13261 aataccatcc ttaaaatcca cttgtattga ttgattcaca gtccttgcat acaatatatt
    13321 attgagtgaa aaggagtgga acagagcgca tgaaggctgt tctcatcagg caatttggtc
    13381 cgcctggggt aatgaagatc gaagaggttg agaagcctgt catatccaga gaccggctac
    13441 tcgttcgcat tttctattcc agcgtgaatc cggtggactg gaagataagg aatggttcat
    13501 taagacttat cagcggttca agatttccca tgatgctcgg ctttgacatt gcgggagagg
    13561 tggtcgatac cgggtccgct gtgacgagat ttaagaaggg cgacagggtt tttggcatgc
    13621 ttgacttcag gcatcgtggg gcctatgccg aatatgcctg tgccaatgag gctggccttg
    13681 ccctcatccc tgaaaccctg gatttcaaag aagctgctgc cattcccctc gctgcactga
    13741 ctgcatatca ggcgttgcac tataaaggca ggataaaaaa cggcaaaact gttcttatca
    13801 atggtgcttc aggaggcgtc ggttcattcg cagtccagat cgcgaaggct tccggcgctg
    13861 cggttaccgc catatgcggc acccacacta ctgaacttgt gaaaaaactt ggagcagata
    13921 gtgtaataga ttatacaaag cacgatttca gacaattctc tgatacgtac gatattattt
    13981 ttgatgtagt ggctaaacat tctttctttg aggtgagcaa gaatctgagg ggtggcggtt
    14041 actatgtcac cacactcccg aacaaaccgg ccgatatcct gagttttgtc ctcactttcc
    14101 ctttatcatt tttggggctc tccaagcggt caacctttat tagtgtccgg ccaaatggcg
    14161 atgatttgca ttcactgagc cttttggtta aagaagggaa gataatgccc ctcattgacc
    14221 gtgagtacac tcttgaggag atcaatcagg cccatgccta tagcgagaaa ggtcatgccc
    14281 ggggtaaaat cctgatccgg gtctcttgaa aaatacctcg gcttgattac atactttctc
    14341 aatgcactca aaaggcttgc ccaaaaagca atcattaaag gagctgtcca gaagataaca
    14401 gaattatccc cattcatcag tcttttgaag agaaggaaat tgaacactct tgtggaaatt
    14461 ggaacggcaa gaggaggtac gttatatgcc tggtgcaaaa tagctgaacc tgatgcgcag
    14521 atcgtcagta tcgatatgcc aggggacccc ttcggaggtg gctattcggc acgggatatt
    14581 cggaagttta aaacgtacaa aaaagagaat cagaagatgt atttcatgag aaaggattca
    14641 cacagaaacg ctacgaagaa tgagttgata aggatactca atggccgcaa gatagatttt
    14701 ctgttcattg atggagatca ccggtactca ggggtaaaga aagactggga attgtattct
    14761 cccctggtga aacagaatgg actcatagtg ttgcatgata tccttgctca tccaaaggtt
    14821 cctcaatgca aagttgatag actatggaag gaacttaaac ttcattatac ccacagggaa
    14881 ttcctcgaca gaagggatga tcgcggctgg ggacagtggg gcggcatagg agtactctac
    14941 tatgtccagc actagggtct cattcaccag cggataacat tacttggtga gtatcttcat
    15001 tatctccctg tatctttcga ctgtcttcga aactatatca ggaggaagta ccggccctgg
    15061 cgaagtcttg tcccagtcca gggtgaggag atagtcacgc acgatctgtt tgtcataact
    15121 gtcctggccc tttcccggct tatacccctt ggcagaccag aaccttgagg agtccggggt
    15181 gagtacctcg tcaatgagca tgagttcgtt gtggtgcata ccgaactcga atttcgtatc
    15241 ggcgatgatg atgcctttct tctcggcaat atcccttgcc tttgaatata tccttaagct
    15301 cgtctgcttc agcttttctg cactctcctt gcccacaatc ttctctgtct cctcaaagga
    15361 tatgttgatg tcatgcccct cttcagcctt tgtggagggg gtaaagatgg gctggtcgag
    15421 ccttgacgat tccacaagtc catggggtat tctgattccg cagatggtgc ctttttcctg
    15481 atattccttc catccgctcc ctgacagata gcctctcact atgcattcca cagccatagg
    15541 ccttgccttc tttaccagca tgctcctgcc ctcgatcatg tccctgtatt tcctgagttc
    15601 cggaggaaac tcatccacgt cagtcgccac aagatggttc ctcactatat cctccgtctg
    15661 tctaaaccag aaaattgata tctgggtcag caccttgccc tttcccggaa tgccgttggg
    15721 aagaaccacg tcaaaggcgg aaatcctgtc ggacgcaata aggaggaggt gctcacccag
    15781 gtcataaatg tccctcactt ttccccgctt tggcatgccg atatccggca tttccgtctt
    15841 taatacaacc gcttccgcac ccatttattc cctcccattg aatcaaaatt acccatgatt
    15901 ttgtgatata atactgtatg gcattgcttg aaatcaaaac atatccggag aaggtgctga
    15961 agcaaaaggc ctctcttgtt gagaagattg acgggaagct ccagcgcctt attgatgata
    16021 tggtggagac catgtatgct gctccgggca tagggctcgc cgccccccag gtgggtattt
    16081 ccaaacagtt aatggtgatt gatgtgagtc tccgggaaga agaaaaaagg cccctgattg
    16141 tccttataaa ccctgagata aagcacgttg agggagagat ggaaagcgaa gagggatgtc
    16201 tgagtcttcc cggatatgta acccaggtga agcgctcaga gaggatagag gtgtgcggtc
    16261 tggacaggac aggtaagcct gtgaagattg agggtgaggg tcttctctcc agggccttgc
    16321 agcacgagat cgaccacctc aacggcacat tgctcatcga ccggataagc agaataaaaa
    16381 gggagttttt caagaagaag ttcaataagg cattgtcaaa ggtttaaccg aaaagatgca
    16441 gagtaacgtc acacttatgc tggccaaacc gtttcagaac ttttataatc gacaaaatat
    16501 ttatatggca catcttcatc tatttaatac catggacatt tccggatctt tcataaggct
    16561 cttttttgaa aggtgccgta taaaagtttt tcgccggcct gcccagcatt cgttttacag
    16621 gagctgattt gtcgatcata ttttttggca cacccgaatt cgcccttccc tctttctggt
    16681 ctttgatcaa ttcgggggaa gagatcagcc tcgtggtaac ccagaccgat aagctcaaag
    16741 gaaggggaca taaactctcc cctccaccgg tgaagaccgc tgcattagag gcagggataa
    16801 gagtcgctca gcctggcaca ttgagagatg acaaattcat tgaggcattg cggttcctca
    16861 agcctgaatt tattgtggta atcgcctatg gtaagatact cccggacgca atcctcgaaa
    16921 cgcccgaaca tggttgctta aatgtgcacg catcccttct gccgaactac agaggtgctg
    16981 cgcctatcgc atgggcgata ctccgtggcg atgaaagaac agggattacc accatgctca
    17041 tggatgaagg gttggacaca ggccctatcc ttttacaaca ggaaacggat atctcccgcg
    17101 atgatactgc gggaagcctg agcgtaaaac tggcccgctt gggtgcttcc ttattaattg
    17161 aaacgctcaa aggaatccgt gatggttctg taaaaccgag ggtccagtcc ggtgaggcaa
    17221 gttatgcgcc acctctgaga aaggaggatg gattgatcga ctggtcaagg agcgctgtgg
    17281 aaatcagcaa ttttgtgagg ggcatgcagc cttggccttc cgcatactgt acgctcgaag
    17341 gtgcaagggt cactatcctg aaggcagagc ccctggaggg gtccggcttg tcagggaccg
    17401 tcataaggag cagtaagggt gagttggtta taggcacggg caaaggtcgt atttccctat
    17461 tggaactcca gccctccggt aagagaccca tgcctgcatc agcctttctc ctggggagaa
    17521 aactcatgga aggaatggtg ttgaaatgat gcgtctgatg agaggcctga cccttaaagt
    17581 tcttttccca ctttttatgc tcctggggtc cttttctgct gagaaaaaag agcggctcca
    17641 gggtctcata atctccctga ataacaaact tctaggcatg gaaaggccaa gggcaaaacg
    17701 gatactcctc ctcctgcccc actgtctcca gataaacgag tgtgatatac gccttaccta
    17761 taacatttat aactgcaaac gctgcggaaa gtgtgaaatc aaggacctga ttcagatcgc
    17821 tgatgacaat aatctgacgc tctttattgc cacaggagga aaccttgcga ggaagatagt
    17881 caaggatgtc cttcctgagg ccattgttgc ggttgcgtgt gaaagggacc tgagcagcgg
    17941 catcgctgat tcctatcctc ttccggttct ggggattccc aacgagcggc ccttcggtcc
    18001 ctgcgtaaat acaagggtga atctggagaa ggtcaaaggc gccatagagt ttttctgcaa
    18061 gaagtagtca tcgggaaagc aatgccagtc ataaaatcca tgcccaccac cagagcactg
    18121 gccctcgcag ctctccgcaa gatatgcgaa caaggcaaga aacccaaagg gatgattgag
    18181 gaactctcgg gctctctgga caagagggaa aggtcctttc tcatggagtt ggtctatggt
    18241 gttttgaggt accgggatac ccttgactgg gcactccacg gatttctcag gaaacctttg
    18301 ggattgagcc aggacacccg gaacaattta cggcttgcag cctaccagat actcttcatg
    18361 cgggtgcagg attgggctgc ggtgaatgaa gcggtggaat tggaaaagcc caaagggagg
    18421 ccggaggtgg tgaatggcgt attgaggagt cttctccgaa agccagccac cgaaagaata
    18481 aaccttaccc ctatggaaag aaaaggcgcc gtcccttata tcgccctcgt cacctctcat
    18541 ccctcatggc tcataaaaag gtgggtgaaa cggttcgggg aaaaaggggc ccgggaactg
    18601 gcagaggcca acaacaggat ccctcccctc accctaaggg caaataccct ccgctccaca
    18661 agggaacaac tcctggaaag gctttccgcc atgggcatca ctggagaacc cacgccatgt
    18721 tcgcccgaag gcataaggct aacaggcttc cctgtgatta aagaactctc cagcctgagg
    18781 gattctttca tagtccagga tgaggcagca cagcttatta cctacctcct tgagccaaga
    18841 cccggggaaa ggatactgga tgcgtgtgcg tccccgggag gaaaaaccac gcatattgct
    18901 cagatgatgg aggacagggg agagatactc gccctggaaa ttgatgaggc aagaatgcca
    18961 cagctgaagg aaaatgtgtc caatctcaat atgaattcaa ttaccttact gcatagggat
    19021 tttcggg
//
LOCUS       DATKOU010000160         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1377602, whole genome shotgun sequence.
ACCESSION   DATKOU010000160 DATKOU010000000
VERSION     DATKOU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377602"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..394)
                     /locus_tag="VMU21_07375"
     tRNA            complement(317..394)
                     /locus_tag="VMU21_07375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(356..358),aa:Glu,seq:ttc)
     gene            complement(481..553)
                     /locus_tag="VMU21_07380"
     tRNA            complement(481..553)
                     /locus_tag="VMU21_07380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Lys,seq:ttt)
     gene            718..2280
                     /locus_tag="VMU21_07385"
     CDS             718..2280
                     /locus_tag="VMU21_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HUO77385.1"
                     /translation="MPSSKKYNMAMLAKIRSASVVGIDAHPVEVEVDITSRGLPHFSM
                     VGLPDVAVKESRDRVRPALKNIGFNFPLKQITVNLAPADLKKEGSSFDLPIAIGIIAA
                     EGAIESGLMDGYLFTGELSLDGRIKPVRGALSMAILAKETGLKGLILPRENAPEAAVV
                     KGVNVFGLESLPGVMEFLTREGEKVPLNIDPEKALEENSLYEEDFSEVKGQEHAKRAL
                     EVAAAGGHNVLTIGPPGSGKTMLSKRLPTILPGMTFEEALESTRIHSVAGLLREGQAL
                     LAMRPFRSPHHTISDVALIGGGQIPKPGEVSLAHNGVLFLDELPEFKRNALEVLRQPL
                     ENGEVTVSRAVASVTYPASFMLVAAMNPCPCGYLSDPKHQCTCSPGQIHRYRRRVSGP
                     LMDRIDIHIEVPAVPYKELSTEISGERSEIIRERVVRARMLQLERFRGDKIYCNGQMK
                     TRHIRKYCKLKDDAQGLLETAMQKLGLSARAYARILKLSRTIADLEAEEDIHSHHIAE
                     AIQYRTLDRGMF"
     gene            complement(2405..>3434)
                     /locus_tag="VMU21_07390"
     CDS             complement(2405..>3434)
                     /locus_tag="VMU21_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HUO77386.1"
                     /translation="GFLVLFDPVKSGIAETIADLHRLGVSLKIITGDNRLVAAHVGQQ
                     VGLLDSEILTGPDLRLMSDEALLKKVNEVDVFSEVEPNQKERIILALKKAKNVVGYMG
                     DGINDASALHTADVGISVDSAVDVAKDAADIVLLEKDLGVLAQGVREGRTTFANTLKY
                     VFMATSANFGNMFSMAGASLFLTFLPLLPKQILLTNLLTDFPEMAIATDNVDKELVES
                     PRRWNMRFIRNFMMTFGLLSSLFDYLTFGVLLFILHASPEEFRTGWFAESVVSASLVV
                     LVVRTRGPFFKSRPGEISVQRNTADRQRCADFAFHSNWRKALWIYPIATLFSCSHGNN
                     CGDLHLCS"
BASE COUNT          866 a          850 c          946 g          772 t
ORIGIN      
        1 tcaacagctt aacctttaag accattgcat gccactggcc cgtgttaggg aagcctcagg
       61 ccggcatgcc aagaatgagg ccttaggcag gtgctgaccc tattattggt gcgccgggag
      121 agttaacaat agggctacgc tctttcaggt gcgggccagc ctggggcctg ctgaccccgc
      181 atggctcagc gccatgcgga ccagctgcct gataatcaga atatggcgag tattatcaag
      241 ggcaggggaa ttgcggcaat tctacggtag tcttagaaaa tgaaaagggg ctacgagtgt
      301 agccccttga attcgttggc gtccccagcg ggattcgaac ccgcgttacc gccttgaaag
      361 ggcgatgtcc taggccgggc tagacgatag ggacatgtaa gaaaagtgat cagcaaagac
      421 ggaaagaaaa cctctaccta ttacctgtca ctctccactt ttcactgaac actcaatcaa
      481 tgagccgcga tggattcgaa ccatcgaccc acgccttaaa agggcgttgc tctgccaact
      541 gagctagcgg ccctctgaat catttaggat acacgcaagg ggaatttatt gtcaatctcg
      601 ccacttgcgg gtgagccgtg aaatagtcag gtggttgagt aggcgggtaa tagctgacta
      661 tttcacgatt tagtggctca actgcttagc gggtttcacc aagaagctga gttgcatttg
      721 ccttcttcta aaaaatataa tatggcaatg ctcgcaaaga tacgaagtgc cagtgtggtg
      781 ggcatcgatg cccaccctgt ggaggttgag gtcgatatca cttccagggg tctgccccac
      841 ttctccatgg tgggtctgcc cgatgttgcg gtaaaggaga gccgtgacag ggtgaggccg
      901 gcgcttaaga atatcgggtt taattttccg ctgaaacaga tcacagtgaa tctcgcccct
      961 gcggacctga agaaagaggg atcttccttt gaccttccca ttgccatagg gatcatcgct
     1021 gcggaggggg cgattgaatc agggctcatg gatggatatc tctttaccgg tgaactttcc
     1081 cttgatggaa ggatcaagcc tgtgaggggt gcgctttcca tggcgatcct cgcaaaggag
     1141 acgggactga agggattaat cctccccagg gaaaatgccc ctgaggctgc ggtggtgaag
     1201 ggggtgaatg tctttggcct ggagagcctt cccggggtaa tggaattcct gaccagagaa
     1261 ggggaaaagg tccccctaaa catagacccc gagaaggccc ttgaggagaa ttccctctat
     1321 gaagaggact tctccgaggt aaaaggccag gaacatgcga agagggcact ggaggttgcg
     1381 gcagcagggg ggcataatgt gctgaccatc ggcccgcccg gttcaggaaa gaccatgctc
     1441 tcaaaaaggc tccccaccat actccccggc atgacctttg aagaggcttt ggaaagcaca
     1501 aggattcata gtgttgcggg acttttaagg gaggggcagg cattgctcgc aatgcggccc
     1561 ttcagatcgc cgcaccacac catctctgat gttgccctga tcgggggtgg tcagatcccg
     1621 aagccgggcg aggtgagcct cgcacacaac ggcgtcctgt ttctggacga actccccgag
     1681 ttcaagagga acgccctgga agttctgagg cagccgctgg aaaacggaga ggtgacggtg
     1741 tccagggcag tggcttcggt gacctatccc gcgtccttta tgctcgtggc tgccatgaac
     1801 ccctgtccct gcggatacct gagcgatccc aagcaccagt gcacctgcag ccccgggcag
     1861 atccaccgtt accggagaag ggtttcgggt ccgttgatgg acaggatcga tattcacatt
     1921 gaggtgcccg cagttcctta taaggagctg tccactgaaa tttcaggaga gaggtccgaa
     1981 attataaggg aaagggtcgt ccgagcacgg atgctccagc tcgaacggtt caggggtgat
     2041 aagatctatt gtaatggcca gatgaaaacg cggcatatca ggaaatactg caaactcaag
     2101 gatgatgcgc aaggcctcct tgagacagcg atgcagaagc ttgggctatc ggcaagggca
     2161 tatgcgcgga ttctgaagct gtcacgcacc atagcagatt tggaagcaga agaagatatc
     2221 cattcgcatc atatcgccga ggcgatccag tacaggacgc tagacagagg aatgttctaa
     2281 tcactggtgc tctgcctttg gtttgcgtag ttattattaa gcttcggaac cccatgccat
     2341 attgttcttg ggagttctta tttttcaaaa ttggactttt ttgtaaaata ccttttttgc
     2401 cgcttcagct gcagagatgt agatcgccac aattattccc atgagaacaa gaaaagagag
     2461 tggcaatggg gtaaatccaa agagccttcc tccaattgga atgaaaggca aaatcagcgc
     2521 aacgctgacg atcagcagtg ttgcgctgaa cagagatttc cccgggtctg cttttgaaaa
     2581 aagggcccct tgtgcggacg acgagcacaa ccagcgatgc tgagacgaca gactccgcaa
     2641 accagccggt cctgaattcc tccggagagg cgtggagaat aaataacaat acgccaaagg
     2701 tgagatagtc aaacagagaa cttaagagac cgaaggtcat cataaagttc cgtataaatc
     2761 tcatgttcca ccgtcggggg ctctcaacca actctttatc cacattgtct gtggcaatag
     2821 ccatctccgg aaaatctgtc agtaaattgg tgagcagaat ttgcttgggc aggagaggaa
     2881 gaaacgtgag aaaaagagat gcccctgcca tactgaacat gttgccgaaa ttggcgcttg
     2941 tcgccatgaa tacatacttg agggtgttgg caaaagtcgt cctcccttcc cgcactccct
     3001 gcgcaagaac tccaaggtct ttttcgagca gtacaatatc ggccgcatcc ttggcaacat
     3061 caacggcgct gtccacagat atgccgacat cagcagtatg gagggccgag gcgtcgttga
     3121 tgccgtctcc catatagccc acgacgtttt ttgccttttt cagggcaagg atgatccgtt
     3181 ctttctggtt aggttcgacc tctgagaaaa catccacttc attcaccttt ttaagcagcg
     3241 cttcatcgct catcagccgg agatccggtc ccgtgaggat ttctgagtcc aatagcccaa
     3301 cctgttgtcc gacatgggcg gcaaccagac gattatcgcc ggtgatgatc ttcagtgaaa
     3361 cgccaaggcg atggagatct gcaatagtct cggcgatgcc tgatttgacc ggatcaaaca
     3421 gtacaaggaa tcct
//
LOCUS       DATKOU010000161        11115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_993993, whole genome shotgun sequence.
ACCESSION   DATKOU010000161 DATKOU010000000
VERSION     DATKOU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993993"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VMU21_07395"
     CDS             <1..305
                     /locus_tag="VMU21_07395"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HUO77387.1"
                     /translation="PTKTALEGEVIHIDHFGNAITNIKVSDLKLLRSASEKGVLRIVA
                     KGKQIPFKQYYSEAEDKGLYALINSMNYLELFIYRGDASSEFNIKVGDTLGVMLVG"
     gene            791..1741
                     /locus_tag="VMU21_07400"
     CDS             791..1741
                     /locus_tag="VMU21_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77388.1"
                     /translation="MARRLLLADDSITIQKVVELVLAEEGYDIKTTGNGEDALSALSS
                     FKPDVVLADIDMPKMNGYQLCEKIKTNPATRNIPVILLAGAFEPLDDELARNVRAEDF
                     IVKPFESQELIDKIHAVLSGETAVKGIAEEEVAVSEVMEGIEPEEDLWTMEEVVSEPL
                     ATEALKEETPVEEVSIEEAFGFAEEAEVGAEKEFLQKPAPARSPERVEVAPPRVEYLS
                     KEEVANIVRREVAEKVSSMLSVDMKDIVLSSLIPSLKDSAEKVLWEVAPDLAEKLLKE
                     MLQGALASLTKEVEKVIWETVPDLAESMIAKEIEKIKSGI"
     gene            1788..4490
                     /locus_tag="VMU21_07405"
     CDS             1788..4490
                     /locus_tag="VMU21_07405"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HUO77389.1"
                     /translation="MTTLEKHYSPQGLEEKWYALWLERGYFSPSPEGSAPYSIVIPPP
                     NVTGSLHMGHALNATLQDVLVRWKRMLGYKALWIPGTDHAGIATQNVVEREIAREGSD
                     RHALGREVFIERVWKWKAEYGGRISHQLKRLGASCDWSRERFTLDEGLSRAVREVFVS
                     LYEEGLIYRDNRLINWCPRCHTALSDLEVEHEEIEGTLTFIRYPLFGGKGNVVVATTR
                     PETMLGDTAVAVNPDDERYQGVIGKNVTLPLTDRKIPIIVDAAVDPAFGTGAVKVTPS
                     HDFNDEAMAKRQKPALPFVTVIGDEGRMMPEAGKRYAGLDRYECRKKVVEDLKASDLI
                     EKEERYRHSVGHCYRCKTIIEPLPTLQWYVNTQSLAKEAMDVVRRGKIRIIPSSWENT
                     YFAWMENIKDWCISRQIWWGHRIPVWYCDECGEVIVSRETPKGCRACKGAALRQDEDV
                     LDTWFSSALWPFSTLGWPDKTQDLTTFYPTSVLVTAFDILFFWVARMIMMGLKFMGEG
                     PFREVYIHAIVRDPQGEKMSKSKGNVIDPLIMVDKFGTDAFRFTLAAFAAQGRDIRFS
                     EERVEGYRHFVNKLWNAARFITMNLKDMDARDLQIIKGQGGPLLTSESVSLDLPGRWI
                     LSRLSVAVQEINDSLKEYRFNDASSAIYQFIWHEFCDWYIEMSKHALYEGGRDKPSTI
                     ACLVSVLDHALRLLHPFMPFVTEEIWQTLRTYLPEGAESIVISPYPESLPRDLRAEEE
                     MSLVMEAITGIRNIRGELNISPATEVKALFKTFRDEVGGILERNLHLIQKLTKANEIT
                     IGKDIDKPKGSATGVRASFEVYVPLEGVLNIEEEVGRMMKEKTKVVESLSFLDKKLTS
                     EDFIKRAPKEVVEKERARYQELVLKDERIEGTIQRLKEMGGKHD"
     gene            4483..4968
                     /locus_tag="VMU21_07410"
     CDS             4483..4968
                     /locus_tag="VMU21_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77390.1"
                     /translation="MIESKAKEIEGEDLEFIEAEFVNIKQSSIRSVEGGHVELQQVGA
                     LSIDGERVEVTQSASAITHGGDVDINQSVSFITAGNTTSLNYSFSSFSLARDFATVNR
                     SAIGLIGAREVHAEHTSAIMLIANKVEGEVTTILDWRSALALGAVVGGALGFLSLFRK
                     R"
     gene            4978..5814
                     /gene="nadC"
                     /locus_tag="VMU21_07415"
     CDS             4978..5814
                     /gene="nadC"
                     /locus_tag="VMU21_07415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HUO77391.1"
                     /translation="MHIPFSVTNILRLALEEDLGNGDLTTTLLIPEEQESRALLIAKG
                     SFIVAGMPFVREVFTLGSPNVGFKIFCDEGDKVKRGEIIAELNGNTRTLLSYERVALN
                     ILQCLSGIATLTNAFVNEVKGTGAMIVDTRKTAPGLRFMEKYAVRVGGGGNHRFGLYD
                     GILIKDNHIEAAGSITSAVRDARKAHHLLRIEVEVENLNDLMEALAAGADVVMLDNMS
                     LTDMKEAVKRAQGKVIIEASGNITIDKVRAVAETGVDLISIGAITHSAPAADISMKIV
                     RQ"
     gene            complement(5890..6867)
                     /locus_tag="VMU21_07420"
     CDS             complement(5890..6867)
                     /locus_tag="VMU21_07420"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77392.1"
                     /translation="MKPTFHHRLVNSQYEDPCLFVRLLRERRAFLFDAGHIDRLSSGD
                     LLKITDVFVTHMHIDHFVGFDTILRALLRRETPLKVYGPKDIIECVEGKLRGYTWNLI
                     EEYPLKLEVFSITDREISHSSFYAENRFKRIDRDVAAFSGIVFQEPPFSVKAAVLSHG
                     IPCLGFALEEEFHINIDKAALHSMGLPVGPWLSELKRMIRNQASSDTLLTINGREYPL
                     SDLITVAMVTSGQKVSYITDISPDENNLNKVVELVRDSDTLYCEAYFLHGDLERAFER
                     HHLTAKITGEIARKSKVKDLVLIHFSPKYFDNPDAIQTEAIGEYRTPQE"
     gene            7005..8003
                     /gene="galT"
                     /locus_tag="VMU21_07425"
     CDS             7005..8003
                     /gene="galT"
                     /locus_tag="VMU21_07425"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HUO77393.1"
                     /translation="MPELRKDPISGGWVIIAVERGKRPTDFASPPVQRKKGGFCPFCP
                     GNEYTTPSEIMAFRPSSTQPNTAGWTLRVMPNKFPALQIYGDLGRSGEGMFDKMNGVG
                     AHEVIIETPDHQLSLASMTLKALEDVLWAYYLRLSDLKKDSRFKYVLIFKNEGDAAGA
                     SLEHSHSQLIALPIVPKLVKEEVDSSRHYFEFKERCIFCDVINQEMDDGKRVIYENSG
                     YIAIAPFAPRAPFETWILPKKHESVFNPPNKSFSALADTLQRVLKQMDRILDTPPYNF
                     VIHTSPFTEEINEYYHWHIELMPKLTNIAGFEWGSGFYINPTLPEEAARFMREAKV"
     gene            8058..9500
                     /gene="glgA"
                     /locus_tag="VMU21_07430"
     CDS             8058..9500
                     /gene="glgA"
                     /locus_tag="VMU21_07430"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HUO77394.1"
                     /translation="MKILIATPEATPYIKTGGLADVTGALLKEYTKMDLQPSIVLPLY
                     RQIREHFKLADTGHRVAVPMGNRTVSGSILSHGRSAYFLECDEFFDRSDLYGTPGGDY
                     PDNAARFTFFARAALEACIALRLEPDVIHCNDWQTGLIPLYMKTLYRSNLFSRTATLM
                     TIHNLGYQGLFDVSSLPLTGLPAEWFNPEGIEFYGKINFLKAGLVASDSITTVSNTYA
                     KEILTPEYGYGLDGFLRKRASVIHGIINGIDTDEWDPFKDTFITAKYGATDFSGKKVC
                     KNDLMRECSLSVHGRDIPLLSFIGRLSAQKGLDILIEAADDIMAAGVNVIILGKGDEE
                     IQDNILSLARRHKGRMFVKIGYDEAFAHRVYAGSDLFVMPSRYEPCGLGQLIAMRYGT
                     IPVARRTGGLEDTITDYKPLRGSGTGFLFDDYRASSLEDCLGRALCAYMDKQRWRKLI
                     SGAMAQDFSWERSAKEYITLYADAIQRKRG"
     gene            9508..>11115
                     /locus_tag="VMU21_07435"
     CDS             9508..>11115
                     /locus_tag="VMU21_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUO77395.1"
                     /translation="MSLRSKLTVFAIGLLLVVAASVTVSFLIFERLSSNFEIMKRSVE
                     EYNLHRGLRSSIVMYLNIPDGWFMSGDRRLRAKYRERLAEVYESFGNLDRFTKEREMI
                     SAIGRDFDEMKRHADAIMSFENPVGNAAVSREVLMLHNGSETLIAELDALYLNSLREL
                     SIRIGQAERVKSEMEFYFTTLFIGSSLTFLLLVLFMRKMIAVPFNDILTATSRITSGE
                     IGYRIASKRSDEFGIIANRFDQMVDELQKTSEENAELYLSTRDQLQKLKAMHELARAI
                     TSTLDLDALLKNIAEDATESFHARGCVIRLLEDDKLMVRASFGVSKELEGMMTLAVGE
                     GLPGKVAAEGRPILVEDLSKMPAEWKIPYLDARSVLNVPLKVGERVIGTWALYDKIGP
                     SGETISFSGEDLTMAEGFASLSAVAIEKAKTFEQELKREREALVAKKRLDVLFESVQG
                     GIITLGKDYTILSANRYMETWIGRPVDEIVGKDCVELVHENKGVCPHCVAQLTFETGE
                     INTITQMSGLNYAELTSYPIKDEHGGIG"
BASE COUNT         2923 a         2473 c         3066 g         2653 t
ORIGIN      
        1 tgcccacaaa gacggcactg gagggcgagg taatccacat agatcacttc ggcaatgcaa
       61 tcacgaacat caaggtgtcg gacctgaaac tcctccgcag cgccagtgag aaaggggtgc
      121 tgagaatcgt ggcaaagggg aaacagattc ccttcaagca atactacagt gaggcggaag
      181 ataaaggtct ttatgccctc atcaacagca tgaattacct tgagctcttc atctaccggg
      241 gggatgcatc ttcagaattc aatatcaagg tgggtgacac cctgggagtg atgcttgtgg
      301 gatagggata aggtctgagg aaatgcacaa tccttggttg ccgttacggg tgttcatttc
      361 gttgactcaa cctcgacgat tattgtaaaa taagtgaaac aatggagggt taagatgccg
      421 atatatgagt atacctgtat aaagtgtaac aaggacttct ctgctttgca aagagtaggt
      481 tgcacggaaa aagacactca atgtccggag tgtggttctc ataatgtgaa gaaaaaagtt
      541 tccgcatttt gctgttcccc tggaggtacg tcatctccat cctcttcagc cccccgcttt
      601 accggaggcg gttgaggcgt ctcctcctgc tgaaccgttg gttcagggaa tcatacgtaa
      661 atacctaaat aagaaaaaat cttgacattt ccccccctat aacaatacaa ttggctgtgg
      721 agcatgcaag ggacgttgtc ccttgactat atcgtgttag aggggtggca tcccccagag
      781 ggaagggaat atggcacgca gacttctcct tgctgatgac agtatcacaa tccagaaggt
      841 ggttgaactt gttctcgctg aagagggata tgatatcaag accacgggta acggcgagga
      901 tgcactctct gccctttctt cctttaaacc cgatgtcgtc ctcgctgaca ttgatatgcc
      961 caagatgaac ggataccagc tgtgcgagaa aataaaaacg aatcccgcaa cgaggaatat
     1021 ccctgttatc ctcctggcag gtgcctttga acctctggac gacgaactgg cgaggaacgt
     1081 gcgtgcagaa gattttattg tgaagccctt tgaatcccag gaactgattg acaaaatcca
     1141 tgcggtcctt tccggggaaa cggcagtaaa gggcattgca gaggaggaag tggctgtttc
     1201 ggaagttatg gagggaatag agccagagga ggatctctgg acaatggaag aggttgtctc
     1261 agagcccctc gctacagagg cacttaagga agagactcct gtcgaggaag tgagcataga
     1321 ggaggccttt gggtttgcgg aggaggctga ggtgggggct gaaaaagagt ttcttcaaaa
     1381 gcctgctcca gcacggtctc cggagagggt ggaggtcgcg cccccccggg tcgagtatct
     1441 gtccaaggaa gaggtcgcca atatcgtcag aagggaggtt gctgagaagg tctcttcaat
     1501 gctctctgtt gatatgaagg atatagtact ttcatccctt atcccctcat taaaggattc
     1561 cgcagagaaa gtcttgtggg aggttgctcc ggaccttgca gaaaagttgc ttaaggagat
     1621 gctccagggc gcccttgcgt ccctcaccaa ggaagtggaa aaagtgatct gggaaaccgt
     1681 tcccgacctc gcagagagca tgattgccaa ggagatagag aagatcaaat ccggcattta
     1741 gcacggtctt tctcctccaa cattgctgag tgattctttc ccctcgtatg acaactttag
     1801 aaaagcatta cagcccccaa ggactagaag aaaagtggta tgccctttgg cttgaaaggg
     1861 gatatttcag tcccagccct gaaggaagtg ccccgtattc catagtaatc ccccccccga
     1921 atgtgacagg ctcattgcat atgggccatg ccctgaatgc cacattacag gacgtgcttg
     1981 tcagatggaa gaggatgctg gggtacaagg ccctctggat acccgggacc gaccatgcag
     2041 ggattgccac gcagaatgtt gtggaaaggg aaatcgcccg cgaggggagt gaccgtcatg
     2101 cgctgggacg ggaagtattt attgaaaggg tatggaaatg gaaggctgaa tatggcggca
     2161 ggataagcca tcagctgaaa aggcttggag cgtcgtgtga ctggtcaagg gagaggttca
     2221 cccttgatga aggtctttcc agggcagtga gagaggtttt tgtgagtctg tatgaggaag
     2281 ggctcattta cagagacaac aggctcatca actggtgtcc caggtgccat accgcgcttt
     2341 cggaccttga ggttgagcat gaagagattg agggtaccct cacctttatc cgttatccgc
     2401 tctttggcgg gaaagggaat gtggtagttg cgacgacgag gccggagacg atgcttgggg
     2461 atactgccgt tgcagtaaat ccggacgacg agcgatatca aggggtcata gggaaaaatg
     2521 ttacgctgcc cttgaccgac agaaagatac ccataatcgt tgacgcagcc gtggacccgg
     2581 cattcggaac aggtgcagtg aaggttacgc cctcccatga ttttaatgat gaggccatgg
     2641 ctaaacgcca gaagccggca ctgccttttg ttaccgtaat tggcgatgag ggaaggatga
     2701 tgccggaggc ggggaagaga tacgcgggct tggaccgata cgaatgcagg aagaaggtcg
     2761 tggaagacct taaggcgtcg gaccttatag aaaaagaaga gcggtacaga cactcggtag
     2821 gccactgtta ccgctgcaag acgatcatag aaccgttacc cacgctgcag tggtacgtga
     2881 acacgcaatc tcttgcgaaa gaagccatgg acgtagtgag gagaggaaag atacggataa
     2941 ttccctcatc ctgggaaaat acctattttg cctggatgga gaatatcaag gactggtgta
     3001 tatccaggca gatatggtgg ggacacagga tccctgtatg gtactgtgat gaatgcggtg
     3061 aagtgattgt ttccagggaa actcctaagg gatgcagagc atgcaagggt gccgctctca
     3121 ggcaggatga ggatgtgctg gatacatggt tctcttccgc actctggccc ttctctaccc
     3181 tcggctggcc tgacaagacc caggacctca ctacctttta ccccaccagt gtgctcgtca
     3241 ccgcctttga tatcctcttc ttctgggttg cgaggatgat catgatgggg ctgaaattta
     3301 tgggtgaggg gccattcagg gaggtttaca ttcatgctat tgtgagggat ccacaaggag
     3361 agaaaatgtc taaatcaaag gggaatgtca ttgatcccct cattatggtc gataaattcg
     3421 gcacagatgc tttccggttt accctagcag ctttcgcagc ccagggaagg gacatccggt
     3481 tctccgagga gcgggtcgag ggataccgcc attttgtgaa caagctatgg aatgcggcac
     3541 gttttatcac catgaacctc aaagacatgg atgcccggga cctgcagatt atcaaggggc
     3601 aggggggtcc tttgcttacc tcggagagtg tctctctgga tcttcccggc agatggatcc
     3661 taagcaggct ttccgtggct gttcaagaga tcaacgactc gcttaaggaa taccggttta
     3721 atgatgcgtc aagcgctatt taccagttca tatggcatga attctgtgac tggtatatag
     3781 agatgtcaaa gcatgcactc tacgaggggg gaagagataa accttctacc atcgcctgcc
     3841 ttgtctctgt tcttgaccac gcgctgcggc ttctccaccc gtttatgccc tttgtaaccg
     3901 aggagatatg gcagacccta aggacctacc ttcctgaagg agctgaaagt atcgtgattt
     3961 ctccctatcc cgaatccctg cctcgagacc tccgggctga agaggagatg tctcttgtta
     4021 tggaagcaat cacaggcatt agaaatatca ggggggaatt gaatatctcc cctgctacag
     4081 aagtgaaggc cctgtttaag accttcaggg acgaagtggg gggaatactt gagagaaacc
     4141 tgcaccttat ccagaagctc acaaaggcga acgagataac cataggaaag gatatagaca
     4201 aaccaaaggg aagtgcaacc ggagtgaggg cctcctttga ggtctatgtt cctctggaag
     4261 gagtattgaa tatagaggaa gaggtcggaa ggatgatgaa agagaaaaca aaggttgttg
     4321 agtcactgag tttcctagac aaaaaactga caagcgaaga cttcataaaa cgggctccga
     4381 aagaggtagt ggaaaaggaa agggcgaggt atcaggaact cgtccttaaa gacgagagga
     4441 tcgagggaac cattcaacgc ttaaaggaaa tggggggaaa acatgattga gtccaaggca
     4501 aaagagattg agggtgagga tctggagttt atagaggcgg aatttgtaaa catcaaacag
     4561 agcagtatca ggtcggtcga agggggccac gtggagttgc agcaggtggg cgcactgagc
     4621 attgatgggg agcgcgttga agtgacacag agtgcgtctg ccatcaccca tggaggtgat
     4681 gtggatatta accagagtgt aagcttcatc acagcgggaa acaccaccag cctcaactat
     4741 tccttctctt cgttttcgct tgcgagggat ttcgctacgg tgaaccggag cgcaataggc
     4801 ttgatcggcg cccgtgaggt gcatgcagag cacacctcgg caatcatgct catcgccaat
     4861 aaggtggaag gggaggttac cactatcctt gactggcgat cggcccttgc cctgggcgcg
     4921 gtggtggggg gagcactggg ttttctctct ctgttcagaa agaggtagcg agcgctgatg
     4981 cacattcctt ttagtgtgac gaatattctc cgccttgccc tggaagaaga cctgggaaac
     5041 ggcgacctca ctacgacact ccttatccct gaggagcagg aatctcgggc actgctgatc
     5101 gcaaagggta gttttattgt tgccgggatg ccatttgtga gagaggtgtt tactctaggc
     5161 agcccgaatg tggggttcaa gattttctgt gatgaagggg ataaggtaaa aagaggtgaa
     5221 attatcgccg agcttaatgg gaacacaagg actcttctct catacgagag ggtggccctt
     5281 aatatactcc agtgcctttc aggaatcgca accctcacca atgcgtttgt caatgaggtg
     5341 aagggaacgg gtgcgatgat tgtagatacg agaaaaacgg ctcccggcct gaggtttatg
     5401 gaaaaatacg ctgtcagggt agggggtggc ggcaatcacc gtttcggtct ttacgacggg
     5461 atactgataa aagataatca catagaagct gcgggcagta tcaccagcgc tgtacgcgat
     5521 gcccgcaagg cccaccatct cctgaggatt gaggtggagg tagagaatct taatgatctt
     5581 atggaagccc tggctgccgg tgctgatgtg gtgatgctgg ataacatgtc tcttacggat
     5641 atgaaagagg cggtgaagcg cgcgcagggc aaggtgatta ttgaggcgtc cggaaatatc
     5701 acaatagaca aagtcagggc agtggcggaa accggggttg atctcatatc cattggcgcc
     5761 attacccatt ctgctcccgc agctgacatc agcatgaaga tcgtaaggca atagcacact
     5821 tcacgctttt taattgactc gtatgggaac tcttgagtgc cgaaaggcga agaagcactt
     5881 accgtgcgct tattcttgag gagttctata ttccccaatg gcctctgtct gaatggcatc
     5941 aggattatca aaatattttg gcgaaaaatg aataagcacg aggtctttca ccttagactt
     6001 gcgagcgatc tctcccgtga tctttgcggt gagatggtgc ctttcaaatg ccctttcgag
     6061 atctccatgg agaaaatatg cctcacagta aagggtatct gaatccctga caagctcaac
     6121 tactttgttg aggttattct catcagggga aatatcggtt atgtaagaga ccttctgccc
     6181 tgaggtgacc atggcaacag tgataaggtc agacagggga tattcccttc catttatggt
     6241 caaaagggtg tcggaactcg cctgattacg gatcattctt tttaattcac tcagccatgg
     6301 gcccaccggg aggcccattg agtgcagtgc agccttatca atgttgatat ggaactcttc
     6361 ttccagagcg aacccaagac aggggatccc atgggagagg acagctgcct ttacgctgaa
     6421 tggaggttcc tggaagacga tgccgctgaa ggctgccaca tcccggtcga ttctcttaaa
     6481 cctattttct gcgtagaaac tggaatggct gatttccctg tcagtaatac tgaatacctc
     6541 aagtttcaag gggtactctt ctataagatt ccacgtgtac cccctcaatt tcccttcaac
     6601 acactctata atgtctttcg gcccgtatac cttcaagggg gtctctcttc ggagcagtgc
     6661 ccggagaatc gtatcaaaac ccacgaaatg gtcaatgtgc atgtgggtca cgaatacgtc
     6721 ggtgatcttc agcaggtcac cggagcttaa acggtcaatg tggccggcgt caaagagaaa
     6781 tgcgcgccgc tcccggagca gccgaacaaa aagacaggga tcttcatact ggctgtttac
     6841 cagtctgtga tgaaaagtgg gtttcatacc ggatgactaa gagattatat actattgtga
     6901 tataatagtg aaatttcaag gcaaaagatc aaaagcagaa atcatttatt caaagcacgc
     6961 agtaaagttt ttaagtatga ttttttcgaa gtacgggagg gtgtatgcct gaattgagga
     7021 aggaccctat ttccgggggc tgggtcatca tcgcggtgga gaggggtaag agaccaaccg
     7081 attttgcttc tcctcctgtg cagaggaaaa aagggggctt ttgtcctttc tgtccgggca
     7141 acgagtacac gacaccctcc gagatcatgg ccttcaggcc ctcttccacc caaccgaata
     7201 ctgccggctg gacattacgg gtgatgccga acaagtttcc cgcactccag atctacggtg
     7261 atctgggcag gagcggggag gggatgttcg ataagatgaa cggagtgggt gcccatgaag
     7321 tcattataga gacacccgac caccagctct cccttgcatc catgaccctt aaggcgctgg
     7381 aggatgtgct ctgggcatat tatctgagac tgagtgacct gaagaaggat tcacgcttta
     7441 agtatgtgct catattcaag aatgagggtg atgcggcagg tgcgtccctt gaacactccc
     7501 actcccagct catcgccttg cccatcgtgc caaaactcgt aaaggaagag gtggattcct
     7561 ccagacacta ttttgaattt aaggaacgat gcatattctg cgatgtgatt aatcaggaga
     7621 tggacgatgg gaaacgggtg atctacgaaa attccgggta tatcgccatt gcacccttcg
     7681 ctccccgggc gcccttcgag acatggatac ttcccaaaaa acacgaatcg gtgttcaacc
     7741 cgcccaataa aagtttttca gcccttgcag ataccctgca gagggttctc aaacagatgg
     7801 acaggatact ggatacccca ccctacaatt ttgttattca tacgtcgccc tttactgagg
     7861 agataaatga atattatcac tggcatatcg aattgatgcc gaagcttacg aacattgcgg
     7921 gatttgagtg ggggtcgggc ttttacataa accccacgct tcctgaggag gcagcacgct
     7981 ttatgaggga ggcaaaggta tagcattcag gatgctgtat aaggattgaa catcaggcgc
     8041 aaggacaaga agttcccatg aagatactca tcgcgacccc agaggcaact ccctatataa
     8101 aaacaggagg gcttgcggat gtcaccggtg cgctccttaa ggagtacaca aagatggacc
     8161 ttcaaccctc cattgttctc cccctctacc ggcagatacg ggagcatttt aagcttgccg
     8221 atacagggca ccgtgtggct gtcccaatgg gaaatcgtac agtaagcggg agcatacttt
     8281 cccatggtcg gtccgcatac ttcctggagt gcgatgaatt ctttgacagg agtgatctct
     8341 acggaacacc aggaggggat taccccgaca atgccgcaag atttaccttc tttgcgaggg
     8401 ctgcactgga ggcgtgcatt gcattgaggc tggaacctga tgtgatacac tgtaatgact
     8461 ggcagacagg cctcatccct ctctatatga aaacgcttta ccgcagtaac ctcttcagca
     8521 ggacagcgac tcttatgacc attcacaatc tcggatatca ggggttgttc gatgtgtcct
     8581 ccctccctct caccggtctc cccgcagaat ggtttaatcc tgagggcatc gagttctacg
     8641 gaaagatcaa ctttctcaag gcaggactcg ttgcttcaga cagcatcacc acggtgagca
     8701 acacctatgc gaaagagatc cttacccctg aatacgggta tggcctggat ggcttcctaa
     8761 gaaaacgtgc gtctgtaatc cacggaatca ttaacgggat agacactgat gagtgggacc
     8821 cttttaagga tacctttatc actgcaaagt atggtgctac tgacttttcc ggaaagaagg
     8881 tgtgcaagaa cgaccttatg agggagtgct ctctttctgt tcatggcagg gatatcccgc
     8941 tcctgtcgtt cataggaagg ctttcggcgc agaaaggact ggatattctt attgaagctg
     9001 ctgatgatat catggctgca ggcgtaaatg taattatcct cggtaagggt gatgaggaaa
     9061 tccaggataa catcctgagc cttgccagga ggcataaggg gagaatgttt gtgaagatag
     9121 gatatgacga ggcattcgcc catcgggtgt atgcggggag tgaccttttc gtgatgcctt
     9181 cacgatatga gccgtgcgga ctgggccagc tcattgccat gagatacggg acgatccctg
     9241 tggcaaggag gacgggcggt cttgaagata cgataacaga ttacaagccc ttaaggggct
     9301 ccggtaccgg atttctgttt gacgattaca gggcttcatc ccttgaggac tgccttggac
     9361 gtgccctgtg tgcttacatg gataaacagc gatggaggaa attaatttca ggcgcaatgg
     9421 cacaggattt ctcatgggag cgctcagcga aagagtatat tactctttat gccgatgcaa
     9481 tacaaaggaa aagggggtag ggatctcatg agtttgcgtt caaaactcac ggtctttgcc
     9541 atcggcttgc tccttgtggt cgcagcctct gttactgtga gctttcttat ctttgagcgg
     9601 ctaagcagca actttgagat aatgaagaga tctgtcgagg agtacaatct tcatagagga
     9661 ctgaggtcat ccattgtcat gtatctgaat atccccgatg ggtggtttat gagcggagac
     9721 aggaggttaa gggcgaaata tcgtgagcga cttgccgaag tgtacgaatc ctttggaaat
     9781 ctcgataggt ttaccaaaga aagggagatg atcagcgcca tagggaggga ttttgatgag
     9841 atgaagcgtc atgcagatgc catcatgtct tttgaaaatc ctgtgggcaa tgctgcggtc
     9901 tcccgtgaag tgctcatgct gcataacggg agtgaaacac tcatagcgga actcgatgca
     9961 ttgtatctca attcattaag agagttatcc attcgcattg gccaggccga acgtgtcaag
    10021 agcgagatgg aattctattt taccaccctc tttatcggaa gttccttgac ctttctgctc
    10081 ctcgtccttt tcatgaggaa gatgatcgcg gttcctttta atgatatctt gaccgcaaca
    10141 agtagaatca cctcaggaga aattgggtat cggatcgctt cgaaaaggag cgatgaattc
    10201 ggtatcatcg ctaatagatt tgaccagatg gtggacgagc ttcagaagac gagtgaagag
    10261 aatgctgagc tctatctttc tacgagggat caattgcaga agctgaaggc aatgcatgaa
    10321 cttgccaggg ccattacctc tacccttgat ctcgatgcac ttttgaaaaa tattgcagaa
    10381 gatgccaccg agagttttca tgcaaggggc tgtgtaatcc ggctccttga ggatgataaa
    10441 ttgatggtaa gggcatcctt tggtgtgtcg aaggagctgg agggcatgat gacccttgct
    10501 gttggcgagg gacttcccgg aaaggttgcc gccgagggaa ggcctatcct tgtggaagat
    10561 ttgtcaaaga tgcccgctga gtggaagata ccgtatctcg atgcacggtc cgtgctcaat
    10621 gtgcctttga aggtaggtga gagggttatc ggcacctggg ccctctatga caaaatcggg
    10681 cccagcggag aaacaatttc cttttcaggg gaagacctaa ccatggcaga agggtttgcc
    10741 tcgctttccg ccgtagccat tgaaaaggca aaaacgtttg aacaggagct gaagagagag
    10801 agagaggccc tggtggcaaa aaagagactg gatgtccttt ttgaaagcgt ccagggaggg
    10861 ataataactt tgggaaagga ttatacgatt ctctctgcga accggtatat ggagacgtgg
    10921 attggaagac ctgtggatga gattgtaggg aaagactgtg ttgaattggt ccatgaaaac
    10981 aaaggggtat gtccccattg cgtagcgcag ctaacctttg agacaggaga gattaacacg
    11041 atcacccaga tgagcggcct gaactatgca gaacttacct cgtatcccat caaagatgaa
    11101 catggcggta tcggg
//
LOCUS       DATKOU010000162        13230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5221210, whole genome shotgun sequence.
ACCESSION   DATKOU010000162 DATKOU010000000
VERSION     DATKOU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 13230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13230
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5221210"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VMU21_07440"
     CDS             <1..748
                     /locus_tag="VMU21_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUO77396.1"
                     /translation="SLFARHFPSVVFYRYLLAIVFRSSAKAKRSLYDSGAWCASSLEV
                     LRALEYVGVNIEITGADNFLTLDGPCVFVANHMSTLETFVLPSIIGPFKSLTFVVKQG
                     LVDYPVFKYVMRSRDPITVRRENPRDDLRAVLEGGAEKLKAGWSLIIFPQSTRYSTFD
                     PKVFNSMGIKLAKRANVPVIPIALKTDAWAEGKHIKDFGRVYPSKKVHFAFGKPLWVK
                     DRGTEEHNEVISFIISKLKEWKGLVADQDL"
     gene            complement(779..2311)
                     /gene="nifA"
                     /locus_tag="VMU21_07445"
     CDS             complement(779..2311)
                     /gene="nifA"
                     /locus_tag="VMU21_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HUO77397.1"
                     /translation="MIRNYNKQVEITALYEISKLLGVSLNLKSNIRGVMKVLSEYLEM
                     KRGTVALRSNNEVSVIAAHGMSEEEIRKGRYRLGEGIIGRVAKLGSPIVIPNIGDEPL
                     FLNKTGARKMIKKENIAFLCVPIKFKDEILGVLSVDRLFGSKGISFEEDLRLLKIIAS
                     LIAQSVKLHREVEREREAFLEEKEKLTRQLKGKYRVENIIGLSDRMQEVFEAIHHVAP
                     SKANVLLRGESGTGKELVAKAVHFMSQRAKGPFIKFNCASIPEGLLESELFGHEKGAF
                     TGAMTARKGRFELAEGGTIFLDEIGDLPLTLQPKILRVLQEREFERVGGERTVKVDVR
                     LITATSRNLEELVSEGKFREDIYYRLNVVPLFLPSLRERKEDIPLLAEYFMKKYNQEN
                     QKSVTIGPDVLDLFTKYDWPGNVRELENTIERMVVMARGKVVAANELPLHIRDYSVRT
                     HYAGTFKEALPSAVEDIEKTSILDALEKTGGVQAKAARVLGITSRQIGYKMKKYNLQR
                     PG"
     gene            2479..3681
                     /locus_tag="VMU21_07450"
     CDS             2479..3681
                     /locus_tag="VMU21_07450"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUO77398.1"
                     /translation="MIEIETYKGTAVTEHRVEIVERKGRGHPDFMCDSIMEAVSIALC
                     REYTKTFGTILHHNIDKGLLAAGKTVKTFGGGRLTKRMELTIGDRATFRVLGKEIPVV
                     DIAIDAAKKWLNRNLRFVDPQKHFTYRVVLAPGSEELTDIFARPGKTMAANDTSAAVG
                     YYPLSPTEEVVLELERYLNSKRFKGNYPETGEDIKVMGLRRSDILDLTVAMPLIARYI
                     HSEKVYFERKERVQGDMMTFLKKYDGFRKIGLHLNTLDQRGRGLGGIYLTLLGTSAED
                     ADSGQVGRGNRVNGLISMNRPMGTEAAAGKNPVSHVGKIYNVLAHEIARMVYQEVEGL
                     KEVYILLLSRIGTPIDNPQMASAHVLTEGKLKVKDISRTITAIIERELANINKFCIKL
                     SKGKYPIC"
     gene            3959..5308
                     /locus_tag="VMU21_07455"
     CDS             3959..5308
                     /locus_tag="VMU21_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUO77399.1"
                     /translation="MDTSAVAASAKRKAHINIVLPLFFASVIAYLDRVNIGYAALTMN
                     KDLGLTAQMFGIGAGVFFFGYILFEIPGALIAEKYSPKMWLARIMLTWGVVSGLMAFM
                     TQTWHFYLLRFLLGAAEASLYPVIYASCIPRWFSAKDRARVIALLLTSLQISGIIGAP
                     LAGWLLGVSIFGLKGWQGLFIIEAIPAIIFTFVIVYWMADCPGEARWLTKDEKEFLTG
                     QFEKEVAAKTSARHYTVLQAFREPEVLKLCATYFLWISGFWGFNYWMPQVLQSISGWS
                     NLAIGWLAAIPMAVSLFVMIYIGHSSSKRSEIRWHGAIGLFIGAIGLGIGVFIKNPWI
                     AFFFVILAAIGTYAPFGVWWSYPTTFLSGTAAAGAISLINSCGNLGGFVGPYITGFIK
                     DTTGSFAGAWVYLAFSLICAAIFILTFKKQLPVHLTDLDETQTENLSKTNHDIAPFL"
     gene            complement(5486..5653)
                     /locus_tag="VMU21_07460"
     CDS             complement(5486..5653)
                     /locus_tag="VMU21_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUO77400.1"
                     /translation="MPAYEYVCKDCSKDFVVFLSIKEFDAKPKIKCPHCGSDNAQRKI
                     TGFFAKTSKKS"
     gene            complement(5728..6945)
                     /gene="dinB"
                     /locus_tag="VMU21_07465"
     CDS             complement(5728..6945)
                     /gene="dinB"
                     /locus_tag="VMU21_07465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HUO77401.1"
                     /translation="MSRIIICADMDAFFASVEQKVNPRLRGKPIGVIGSGARTVITTA
                     SYEARKFGVRTGMNVWEARKACPHLILVIANNARYTFTCRGLEEIYRKFTPDMEIYSV
                     DEAFLDVTTTHHLFGGPEEIGRSIKQEIKEKFGIACTVGIGPNILLAKLASDLGKPDG
                     LKWIKPRNVEGMLKDLPVKELWGIGSKITERLAVLGIRTCGELGRTPAGLLRTKFGIY
                     GEALKAMGMGVCTRSVQREPGDAKSIGHSMTLVRDIGEMREIEAYLLKLSEMVGRRAR
                     MHRYMGRKISLIIRYTDFETFSRQTTVQDLTNDTHVIYEAALRVMDGVRLRDRVRLLG
                     VCLSDLTRDPCQIPLIEEERKRRSVLRAMDSMNDKYGEFTLAWGSYIMQERDAGVISP
                     SWRPSGVKSVELK"
     gene            complement(7068..7574)
                     /locus_tag="VMU21_07470"
     CDS             complement(7068..7574)
                     /locus_tag="VMU21_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77402.1"
                     /translation="MKHVPSNSFLNEIDFLEVVVSRKEVESQNVGIVLKTLQRLIENP
                     KVASKFRENVDIAFHGYDSDHAELFEILEVRDFVYELDKHFPYWLYFLSKYHTGLQCI
                     IYCFLPPYLTEEAKKKIYPQRLQDYLINRGLPAMNHICEFTGCSEKAVEQMTERVIKY
                     IQFGRLKY"
     gene            complement(7666..8514)
                     /locus_tag="VMU21_07475"
     CDS             complement(7666..8514)
                     /locus_tag="VMU21_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HUO77403.1"
                     /translation="MKNKNNGEDDDIALLCTSCGVKNKLKASKLHLNPICGKCKKTLM
                     SLEPPSPDRFDVTHARIEHYRKATEKKYVTAIILGIVSAVIIGVADIIPRSIPFLFAL
                     ALAYIPGYIIGYIIEERRILRVKSEPAYSRFLEYQRVVAAHEERTREIRRQEFKRKDA
                     ESKSERWWETIDGHSFELEVTKILLSKGYDVKHTGSQFGDEGVDLLLRLDNKLIIIQC
                     KAFKDYLNAGVVRELYGTLIHQKADEGWLVVTSGFYEGAWDFAKDKPIRLMTIQQVLR
                     LPVLSS"
     gene            complement(8609..9040)
                     /locus_tag="VMU21_07480"
     CDS             complement(8609..9040)
                     /locus_tag="VMU21_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO77404.1"
                     /translation="MKEFVGGISRNTCQDVRDRIIKLSGGNFDPLAVVEKNSNKYIGR
                     CGFIMNKFVGEVEIHIVLKKEMWGKGLGTEIAEALLSLGFNSLGFRRIIGVVHPDNSQ
                     SKHVLKKLGMTYCNDISSDDYTNGFQVYSVETKAGCYHLMT"
     gene            complement(9167..9481)
                     /locus_tag="VMU21_07485"
     CDS             complement(9167..9481)
                     /locus_tag="VMU21_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HUO77405.1"
                     /translation="MKRGEVWWADLPAPLGRRPVVLLSRDEAYSVRNAVTVAEVTRTI
                     RGIPVEVPLGTEDGLPRKCVVNLDTIITIRKELLVERIALLKSDKMKQIKKAIQFALD
                     ME"
     gene            complement(9478..9735)
                     /locus_tag="VMU21_07490"
     CDS             complement(9478..9735)
                     /locus_tag="VMU21_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77406.1"
                     /translation="MSKAVKLAISISSEDFKTIEAIRVKEGTTRSGVVTEAIRLLRDK
                     TEKERLIRLYEKGYRKHPEHLPEIKAKEKAALEVLSQEDWN"
     gene            complement(9839..10081)
                     /locus_tag="VMU21_07495"
     CDS             complement(9839..10081)
                     /locus_tag="VMU21_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77407.1"
                     /translation="MMHVGETISVIASFGLPYKIRPLRFKWSGKLLEVKEVTYLWTEK
                     EGQKNIYHFSVSDGRALYELSFDAVALLWRLEKLEA"
     gene            complement(10078..10677)
                     /gene="lexA"
                     /locus_tag="VMU21_07500"
     CDS             complement(10078..10677)
                     /gene="lexA"
                     /locus_tag="VMU21_07500"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HUO77408.1"
                     /translation="MRELTDKQKRIVEFLKTYVGEHGYPPTMREIGEHFGFTWPAARG
                     HLAAIEKKGAIRMNPMKSRGIEVIGLRPKDVLSLPVAGRIRAGKPLLALEEIESHIVV
                     DRNLFKESNSFVLRVTGESMVDAGIFHGDYVVVKPQKELRGGDIGVVLIGEEATVKKV
                     YPKRGSITLVPANKMMEPVTYRTDEVSIIGKVIGVIRKL"
     gene            10896..11747
                     /locus_tag="VMU21_07505"
     CDS             10896..11747
                     /locus_tag="VMU21_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUO77409.1"
                     /translation="MLKRILGILLLILCAVSLHLALAASDPGDREGGKDPALFSRMPG
                     FHIYNYKELEFDRREFWVGRDKKQPVEGRYYYVDYYANEGIKIPSGLQIVRNYVNAAK
                     AIGGTLVYEWEDGGTQYVTLKVVKDNSEVWAEVSGASNGMYNVNIIEKQLMKQDVVAT
                     AESLAGNINETGKAAVYGIYFDTGKAEIKQGSEPALAEIVKLLNSDSQLKLYVVGHTD
                     NVGLFEYNIKLSQDRAAAVVKVLIGKYGITSSRLTPFGDGPTAPVASNKSEEGRAKNR
                     RVEMVAQ"
     gene            complement(11870..12193)
                     /locus_tag="VMU21_07510"
     CDS             complement(11870..12193)
                     /locus_tag="VMU21_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77410.1"
                     /translation="MNTEFPLSGDVSQMFKLWAKYLSQQTGFINVNNVVSTNPEIEKN
                     IIEEGASYGKQLGFISEALGMVIRKLELDKLPTDLSEEDRKIVVRFTDMMENIQRIKK
                     EKSKE"
     gene            complement(12457..12711)
                     /locus_tag="VMU21_07515"
     CDS             complement(12457..12711)
                     /locus_tag="VMU21_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HUO77411.1"
                     /translation="MSITICLACQIGIRVASIFDIQVKRIHEYKRELLNVLHIIAPGK
                     AKNWVQTVPGKDWNTIPRLYGPLEPWFDKTLRPREIDLVK"
     gene            12781..>13230
                     /locus_tag="VMU21_07520"
     CDS             12781..>13230
                     /locus_tag="VMU21_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUO77412.1"
                     /translation="MLNLYNNFLFTKCFVDFFQKMQLDGIEAARKFWDSYPYKNIYPP
                     NASDIFEKMIDFYIDLSHVPKEQFDALITRSSRKSRRGFDGFLDILSQRKEDTNAFGW
                     GPSRAYPPGTHIVRQDTPAEAVFLVESGLLKLSRIVHTGSEVIAGLRR"
BASE COUNT         3317 a         3429 c         2847 g         3637 t
ORIGIN      
        1 ttccttattc gcaaggcact tcccttccgt agtcttttat cgttatctcc ttgccattgt
       61 gttcaggtca agcgccaagg caaaacgatc cctctacgac agtggagcat ggtgcgcgag
      121 cagcctggaa gttcttcggg ccctggaata tgtgggagtg aacatcgaaa tcacaggcgc
      181 agataacttc ctcacacttg atggcccctg cgtattcgtg gccaaccata tgagcacact
      241 cgaaaccttt gtccttccca gcatcatcgg acctttcaag agtctcacct ttgtcgtaaa
      301 gcagggcctt gtggactatc ctgttttcaa atatgtaatg cgctcacggg atcccatcac
      361 cgttcgtcgg gagaaccccc gggatgattt gagagctgtg ctggagggag gtgctgaaaa
      421 acttaaggca gggtggtctt taatcatttt cccccagtca acgagatact caacatttga
      481 tcccaaagta ttcaacagta tggggataaa attggccaaa agggcgaatg ttcctgtaat
      541 ccccattgcg ttaaagaccg acgcgtgggc tgaaggaaag catattaaag actttggcag
      601 ggtctatccc tcgaaaaagg tccacttcgc ctttgggaaa cccctatggg taaaggatcg
      661 cggtaccgaa gaacacaacg aggtcatatc cttcatcatc tctaaactga aggaatggaa
      721 ggggctggtg gcggatcagg atctctaata ccattctcta aatgcaccct ctattccctt
      781 agccgggcct ctgaagatta tactttttca ttttatatcc tatctgtctt gacgtgatac
      841 caaggacacg ggctgccttt gcctgaaccc ccccggtctt ttcaagagca tcaagaatac
      901 tcgtcttctc gatatcctca acagctgagg ggagcgcctc tttgaaggta cccgcatagt
      961 gtgtcctcac ggaataatcc cttatgtgta acggcagctc atttgcagca acgaccttcc
     1021 ctctcgccat taccaccatt ctctctatgg tattttcgag ttccctcaca ttcccgggcc
     1081 agtcatactt tgtgaagaga tcaagaacgt ccgggccaat ggtaaccgat ttctggttct
     1141 cctggttata tttcttcata aagtattccg caagaagagg gatgtcctcc ttcctttcgc
     1201 ggagggaggg gaggaacagg ggaaccacat tcagacggta gtagatatcc tccctgaact
     1261 tcccttctga cacgagctct tcaagatttc tgcttgtagc cgtgatgagc ctcacatcga
     1321 ccttcacggt cctttcgcca ccaaccctct cgaattccct ttcctgcaaa accctcaata
     1381 tcttgggctg gagggtgagg ggaaggtcac caatctcatc caggaatatg gtccctcctt
     1441 ctgcaagctc aaaacggccc ttccgtgcgg tcatggcacc ggtaaatgcc cccttctcat
     1501 ggccgaagag ctctgattcc agaagccctt cagggataga ggcgcagttg aattttatga
     1561 agggaccctt cgctctctga ctcataaaat ggaccgcctt tgcaaccagt tccttccccg
     1621 ttccgctctc accccttaac agcacattgg cctttgaggg agctacatga tgaatggctt
     1681 caaagacctc ctgcatcctg tctgacagcc cgatgatgtt ctccaccctg tatttcccct
     1741 tcagctgtcg cgtgagtttt tctttctcct caagaaatgc ctccctctcc ctctcaacct
     1801 ctctgtggag ctttactgat tgtgcaataa gggaggcaat gatcttcaat aacctcaaat
     1861 cctcttcaaa agatatccct ttggaaccaa agagccggtc aacgctcaat acccctaata
     1921 tttcgtcctt gaattttatg ggcacgcaca ggaaggcgat attctccttc ttaatcatct
     1981 tcctcgcacc cgtcttgttg aggaacagtg gttcatcacc gatattggga attacaatgg
     2041 gcgaaccaag cttcgccacc cttccgatga taccctcccc cagcctgtat ctgcccttcc
     2101 tgatctcctc ctccgacata ccgtgggcag cgatgaccga aacctcgtta ttactccgga
     2161 gcgcaacggt cccccgcttc atctccaggt actcagagag gaccttcatt acgcccctga
     2221 tgttcgactt cagattcagg gacacaccaa gaagtttgct gatctcataa agtgcggtaa
     2281 tctccacctg cttattgtag ttacgtatca taagttcagt ttaatgaagc aagacaaaat
     2341 tgtaaagggt aaaacgttct tgtaagaagc ttccctgagg ccgtaatgac gcgagcctgc
     2401 agaaaaaagt gtgcgctcct cgctcttgca cgtgttcatg gagtccacaa cagagtcatg
     2461 ttctgctaag atgtatgtat gatcgagatc gaaacgtata aaggaacagc ggtgaccgaa
     2521 caccgggtcg aaatagtgga gagaaaaggg aggggccatc ctgactttat gtgcgactcc
     2581 ataatggagg ccgtatccat agcgttatgc agggaatata caaagacctt cggcacaatt
     2641 ctccaccata acatcgacaa gggactcctc gctgccggca agacggtgaa aacctttggc
     2701 ggaggaaggc tcacaaaacg tatggaactc accatagggg acagggcaac attcagggtt
     2761 ttgggaaaag aaatacctgt cgttgatatc gctatcgatg ctgcaaaaaa gtggctcaat
     2821 aggaatctta gatttgtgga tcctcaaaaa catttcacct acagagttgt cctcgcgcca
     2881 ggctctgaag agctcaccga tattttcgca agacctggaa agacgatggc tgcaaatgac
     2941 acctctgctg cggtgggata ttatcccttg agtcccacag aagaggttgt gcttgaactg
     3001 gagagatatc tcaactcaaa gaggttcaag ggtaattatc ccgagaccgg ggaagacata
     3061 aaagtcatgg gattaaggag gagcgacatc cttgacctca ccgtcgcaat gcccctcatt
     3121 gcgcgttaca ttcattcaga gaaggtctac tttgaaagga aagagcgcgt gcagggtgat
     3181 atgatgacat tcctcaaaaa atatgacggt ttcaggaaga taggactcca tctcaatacc
     3241 cttgaccaaa gaggcagagg actaggaggc atctacctca cgctcctggg cacatctgca
     3301 gaggatgcgg attcaggcca ggtggggcgc ggcaacaggg taaacggtct catctccatg
     3361 aacaggccta tgggaaccga ggcagctgcg gggaaaaacc cggtgagcca tgtgggaaag
     3421 atctacaacg tgctggccca cgaaatcgca cgcatggtct accaggaagt cgagggcctc
     3481 aaggaagtct atatcctcct cctcagcagg ataggaaccc ccatcgataa tccacagatg
     3541 gcgtcagccc atgtattgac cgaggggaaa ctgaaagtta aagatatctc aagaacaatt
     3601 accgctatca tcgaacggga actggcgaat atcaacaaat tctgcatcaa gctcagcaag
     3661 ggaaaatatc ctatctgctg actcttgtcc gtccatcctt tcactcactc ttttcctcag
     3721 gggcaccacc ttagtgggcg tcactacgcc gcagcatagc gattatttta cgctagttgt
     3781 tgttgacata attactacct cctgaaacca gtcctcgtat cataattata cgtgtcaaac
     3841 tgtttcggct tttgagaact ctcggtgcgt cgcataagag atatttcaat gattgtgata
     3901 ctatcctttt gctgacgaga ggataaattt taattataat cttttcagga gattgtagat
     3961 ggacacttct gcggttgcgg caagcgcgaa acgaaaagcg cacataaata ttgtgctgcc
     4021 gctgttcttt gcctcggtca tagcctatct cgaccgggtg aatatcgggt atgcggcgtt
     4081 gacgatgaac aaagaccttg gtctcactgc acagatgttc ggcataggcg caggcgtctt
     4141 ctttttcggc tacatattgt tcgagatacc cggagcgctg atcgcggaaa agtacagccc
     4201 caaaatgtgg cttgcgcgta tcatgctgac ctggggagtc gtgtcggggc tcatggcgtt
     4261 catgacgcag acatggcact tctatctcct gcggtttctg ctcggtgccg cagaagccag
     4321 tttgtatccc gtgatctatg cttcctgcat acctcggtgg ttcagcgcaa aagaccgcgc
     4381 acgtgtgatt gccctcctcc tgacatccct ccagatatcc ggaattatcg gcgcgcccct
     4441 tgcgggctgg ctgctcggcg tatcgatctt cggattaaaa ggctggcagg gactgttcat
     4501 tatcgaggct atcccagcga tcatattcac ctttgttatt gtctattgga tggcggactg
     4561 tccgggtgag gcaagatggc tgactaaaga cgagaaggaa ttcctgacag ggcagtttga
     4621 aaaagaggtt gcagcaaaaa cttcggccag acattacacg gttttacaag cattcaggga
     4681 accggaagtg ctcaagctct gtgcaaccta tttcttatgg atcagcgggt tctggggctt
     4741 caactactgg atgccacaag tgctacaatc catctccggc tggtccaacc ttgcgattgg
     4801 ctggctcgct gcgatcccga tggctgtctc tctcttcgta atgatctaca tcggacattc
     4861 ttcatcgaaa agaagtgaga ttcgctggca cggtgccatt ggtctcttta tcggcgcaat
     4921 cggtctggga atcggcgttt ttataaaaaa cccatggatc gccttcttct tcgtgatcct
     4981 tgccgcgatc gggacatatg ctccgttcgg tgtgtggtgg tcatatccga cgacatttct
     5041 gtccggaacc gctgccgccg gcgccatcag cttgatcaat tcatgcggca acctgggcgg
     5101 atttgtcgga ccctacatta ccggtttcat caaggatacg accggctctt ttgccggcgc
     5161 gtgggtctat ctcgcattct ctttgatctg cgccgcaatc ttcatcctta cctttaagaa
     5221 gcagttgccc gttcatctca ccgatctcga tgagacacaa acagagaatc tttcgaaaac
     5281 gaatcacgat atcgcgcctt tcctctgagg cttttttgac aaactcccct gccctagcaa
     5341 aattagcctc ttttgactcc catgcaatat tcacctggat catggccgct ctcacaggat
     5401 actcgtctcc tctagtcgtt ggtctttatt ttacagggtg aggggaagtg taggggtaaa
     5461 ggattacagc accatctcga cagtgctatg acttcttgct cgtcttggca aaaaagcctg
     5521 taatcttcct ttgcgcatta tcactcccgc agtgggggca tttaatcttc ggctttgcat
     5581 caaactcctt aatggaaaga aaaacgacaa aatccttgct gcagtcctta cacacatatt
     5641 catatgccgg cataatgacc tcctcgattt ccgtagtgac agcccacaat tcactatatc
     5701 ataaaatgcc cgcaatcaca ccaccattca tttcagctca acacttttca ccccggatgg
     5761 ccgccaggag ggtgatatga ccccggcatc tctttcctgc attatataag aaccccaggc
     5821 aagcgtgaac tccccatatt tgtcattcat ggaatccatg gcgcggagga cggacctgcg
     5881 ttttctttct tcctctatga gggggatctg acagggatcg cgggtaaggt cagagaggca
     5941 tacgcccaga agccgcaccc tgtccctcag ccttaccccg tccatcaccc tgagcgcggc
     6001 ctcataaatc acatgggtat cattggtgag gtcctgcacc gtggtctgcc tcgaaaatgt
     6061 ctcaaagtcg gtatacctga tgataaggga tatttttctc cccatatacc ggtgcattct
     6121 cgccctcctt cccaccatct cgctgagttt gaggagataa gcctctatct ccctcatctc
     6181 cccgatatcc ctcacaaggg tcatactatg cccgatggac ttcgcgtctc cgggctccct
     6241 ctgcacagac ctggtacata cccccatccc cattgccttt aaggcctcgc cgtaaatccc
     6301 gaattttgtt ctcaaaagac ctgcaggggt ccggccgagt tccccgcatg tccttatgcc
     6361 aaggaccgca agcctttccg tgatcttcga gccgatgccc cacaactcct ttaccggaag
     6421 gtccttaagc atgccctcca cattcctggg cttaatccac ttaaggccgt caggctttcc
     6481 gagatcactc gcgagtttgg caaggagaat attgggtcct attcctacag tgcaggcaat
     6541 gccgaacttc tcctttatct cttgttttat ggatctccct atttcttccg gtcccccgaa
     6601 aaggtgatgt gtggtggtga catcaaggaa agcctcgtcc accgagtata tctccatatc
     6661 aggggtgaac ttcctgtata tctcctcaag ccctctgcag gtgaaggtgt acctcgcatt
     6721 attggcgatc acaaggataa gatggggaca ggcctttctg gcttcccaca cgttcatgcc
     6781 tgtcctgaca ccgaattttc tcgcctcgta cgatgccgta gtaatgaccg tccttgcgcc
     6841 tgaaccgatt acccctatgg gtttgcccct gagacgcggg ttcaccttct gctccacaga
     6901 ggcaaaaaaa gcgtccatat ccgcacagat gataatacgt gacataattt caccaattaa
     6961 agagcggtgc ggagagggtt cggaaggata ttcataacac gttccacctc aataattgtc
     7021 gctgtccctg tttgttccct ataaaatgag tcgtcctgtt ttcaaattta gtatttaagt
     7081 ctaccaaatt gaatatattt gataaccctt tcagtcatct gttctactgc tttttcacta
     7141 cagcctgtaa attcgcagat gtgattcatt gcaggtagcc cgcgatttat aaggtagtcc
     7201 tgcaatcgtt gcggatatat tttctttttc gcttcttctg tcaaataggg agggagaaaa
     7261 caataaatta tacattgtaa ccccgtatga tattttgata ggaaatacaa ccagtaagga
     7321 aaatgcttgt ctagttcata aacaaagtcc ctaacttcaa gaatttcgaa taattctgca
     7381 tgatcagaat catatccatg gaaagcaata tcgacatttt cacgaaattt tgatgcgact
     7441 ttaggatttt caatcaacct ctgcaaagtt tttaagacaa tacctacatt ttgagactca
     7501 acttcctttc ttgagaccac aacttccaaa aaatcaattt cattcaaaaa agaatttgag
     7561 ggaacgtgtt tcactcctct ccttcacatt taaagtgctg ccataagttt aaatttagtt
     7621 gttttataac aacttccttt ttttttgaaa atacagggaa atcgactaag aggataaaac
     7681 aggtaatctc aacacctgct gtatcgtcat caatcgaata ggcttatctt tggcaaagtc
     7741 ccaggccccc tcgtaaaacc cggaggttac gaccaaccat ccttcatcag ccttttgatg
     7801 aatcaatgtg ccgtagagct cacgaactac tccagcattc agatagtctt tgaatgcctt
     7861 acactggatg ataattagct tattatcaag tctcaacaga aggtcaaccc cctcgtctcc
     7921 aaattggcta ccagtgtgtt ttacgtcata gcccttgctc aaaaggatct tggttacttc
     7981 cagttcaaag ctatggccgt caatcgtctc ccaccatctt tccgatttcg actcggcatc
     8041 tttccgcttg aactcctgtc tcctgatttc ccgtgtacgc tcctcatgtg cagccacaac
     8101 acgctggtat tcaagaaatc tggaataagc aggttcactc ttaaccctta atatgcgacg
     8161 ctcctcaatg atgtagccaa ttatgtaacc tgggatgtat gccaaggcta aggcaaacaa
     8221 aaaaggaatc gaacgaggaa ttatgtccgc cactcctata atgacagcgc taacgatacc
     8281 aaggataatc gcagtgacat attttttttc agtagccttt ctataatgct cgatacgagc
     8341 atgggttacg tcaaatcgat caggactggg aggttcaagg ctcattaagg tttttttgca
     8401 tttcccgcaa atcggattaa gatgaagctt cgaggctttt agtttatttt ttacaccaca
     8461 ggacgtacaa agaagggcta tgtcgtcatc ttccccgttg tttttatttt tcatggctga
     8521 tattgaagat ctcctcttac agtcatttca tcacgtttgt catttaagct ttcaggactc
     8581 agagatcaac tcaaatgcaa tgtctccttc atgtcattag gtgataacat ccggccttgg
     8641 tttctaccga atagacttgg aacccatttg tatagtcgtc actggatata tcattacagt
     8701 atgtcatgcc aagcttcttc aaaacatgtt tgctctgact attatcagga tgtactaccc
     8761 caattatcct tctaaatccc aacgaattga aacccagaga tagaagtgct tccgcgatct
     8821 cagtccccaa gccctttccc cacatttctt tctttaacac tatgtggatt tctacttcac
     8881 ccacaaattt attcataata aacccgcacc gtccaatata tttattactg tttttttcga
     8941 ccactgccaa ggggtcgaag tttccgccag ataacttgat aatcctatcg cgaacatcct
     9001 gacaagtgtt tcttgatata ccaccaacaa actccttaat ctcggcgttc gtttagatct
     9061 catagtatga atcagcatct gtcaccgaca aaggtcgcag caaaagccta tcagtttcaa
     9121 ttggagtatt gattggcaaa acctcctccg ttttggctct tttgtcctac tccatatcca
     9181 gggcaaattg gatagccttt ttaatctgct tcattttatc gctcttgagc agggcaatcc
     9241 tttccacaag gagttctttg cggatagtga tgattgtatc gaggttcacc acgcatttcc
     9301 tcggcagccc gtcttctgtt ccgagcggca cttctacggg gattccccgt attgtcctcg
     9361 tgacttcagc caccgtcacc gcatttctga cagaataagc ctcatccctt gaaaggagca
     9421 ctaccggacg tctgcctagc ggagccggaa ggtcagccca ccagacctcg ccccgcttca
     9481 attccagtcc tcctgcgaca acacctcaag ggcagctttt tctttggcct ttatttcagg
     9541 caaatgctca ggatgttttc tgtaaccttt ttcgtacaat cttatcagtc tttctttttc
     9601 ggtcttgtct ctcagaagcc tgatcgcctc tgtcacaacg ccgctcctcg tggtgccctc
     9661 tttcactctt atcgcctcaa tggtcttaaa gtcctcactg cttatgctta ttgccagttt
     9721 cacagccttt gacataatta actatcacct ccggtataac ctatttcata ccagagtatg
     9781 accaattgta atataaaaat caatagcaat tttcttatat acgctctgta tatccagttt
     9841 atgcctccag cttttccagt ctccagagga gagcaacggc gtcaaagctc agttcatata
     9901 atgcccttcc atcagagaca gagaagtggt aaatgttctt ctggccctct ttttctgtcc
     9961 agagataggt tacctccttt acttcaagga gtttaccact ccacttaaac ctcagcggcc
    10021 ttatcttata gggaagaccg aaggaggcga tcacgcttat ggtctctccc acatgcatca
    10081 taatttcctt atgacaccta tgactttccc tattatgctc acctcgtcag tccggtacgt
    10141 taccggctcc atcatcttgt tggcagggac gagggttatg gagcctctct ttgggtatac
    10201 cttcttcacc gtagcctctt cgccgatgag cacaacccct atatccccgc cccgtagctc
    10261 cttctggggt ttcaccacca cataatcccc atggaatatc cctgcatcca ccatgctttc
    10321 ccctgtcacc ctcaagacaa aggaattgct ctctttgaaa agattcctgt ccacaacgat
    10381 atggctctct atctcctcaa gggcaagaag gggtttccct gcccttatcc tgcctgcaac
    10441 agggagggac aagacatcct ttggcctcaa accgatgacc tcgatgcccc tggatttcat
    10501 ggggttcatg cggatagccc ctttcttttc aatagcagcg agatgccccc tggcagcagg
    10561 ccacgtgaaa ccgaaatgct cacctatctc cctcatcgtg ggagggtatc cgtgctctcc
    10621 cacataggtt ttaaggaatt ccacaatcct tttctgctta tcagtgagtt cccgcatgat
    10681 caaccactac atttacattt gtatactatt taggaatttt tgtcaaggga ttgaatactc
    10741 taaaccttcc cggccctctc gcctttattt ttttattcta tattcaaatg aactttctgc
    10801 tatgttaaaa tgatggcctt cgagagaaga tatctgtcgg atagaataat acttatgaaa
    10861 gcacttaaaa atatattttg gagggaggct ttaccatgtt gaaacgtatc ttaggaattc
    10921 tgcttctgat cttatgtgca gtttcactac atctagccct ggctgcaagc gaccctggcg
    10981 atcgcgaggg gggcaaggat ccggccctgt tcagccgtat gccgggtttt cacatttata
    11041 actataagga gctcgagttt gatcgccgcg aattttgggt gggtcgcgac aagaaacagc
    11101 cggtcgaggg tcggtattat tatgtggatt attatgccaa cgagggcatc aaaatcccca
    11161 gcggcctgca gattgttcgt aactacgtca atgctgccaa ggctattgga gggacgttag
    11221 tctatgaatg ggaagacggt ggcacccagt atgttaccct aaaggtagtc aaggataatt
    11281 ctgaggtctg ggcagaggtc tcaggagcca gcaacgggat gtataacgtc aacatcatcg
    11341 aaaaacagct tatgaagcag gatgtggtgg ccactgccga aagcttggca ggaaacatca
    11401 atgagactgg gaaggctgct gtctacggca tctatttcga taccggcaag gccgaaatca
    11461 agcaggggtc agagccagcc ctggctgaaa tcgtcaagct gctcaattct gattcccagc
    11521 tcaaacttta tgtggtaggc cacaccgaca atgtcggcct atttgagtac aatatcaaac
    11581 tttcccagga ccgggcagcg gcggtggtga aggttctgat cggtaaatac ggcatcacct
    11641 cctcccgcct tacccctttc ggcgacggcc ccacggctcc cgtggcctcc aacaaaagcg
    11701 aggaaggacg agccaagaac cgcagggtgg aaatggtagc ccagtaattt ccctaggctt
    11761 actggcagtt aaggcagggt tgcccgtgtc aggaaaagag ccaatcatga aaaccttaac
    11821 atgatttaac agaatttggt gggaaagtcg aagggtcaga acctgtgctc tactccttgc
    11881 ttttctcctt cttgattctc tgaatgttct ccatcatatc tgtaaacctg acaacaatct
    11941 tacgatcttc ttcagataga tccgtcggca atttatccaa ttcaagtttc cttatgacca
    12001 tgcctaaagc ttcactaatg aaaccgagtt gttttccata acttgcacct tcctcaatta
    12061 tatttttttc aatttccgga ttggtggata caacattatt gacattaata aatcctgttt
    12121 gctggctcaa gtattttgcc cagagcttaa acatctgtga aacatcacct gatagcggga
    12181 attcagtgtt catggaaagc cccccttttt atgtttacta gaataattat accctaatat
    12241 cttaatccga caaagctgtt tcagcttcaa taggcaacat tccttcctga ggccgacagt
    12301 gttacttttt tgtcacaaca caaggtttct gttggtaata gatggttgcc agatagctca
    12361 tctatgcagg gtacgtgtag aacttagcac ataaggcaga tcagtcagga ggaaaagagc
    12421 attttgccct ttccctcctg acgagcctat tttatatcac ttcaccaagt cgatctctct
    12481 cggcctcaat gtcttgtcga accacggctc caacggacca tagaggcgag ggatcgtgtt
    12541 ccagtccttg ccaggtacgg tctgcaccca gttcttcgcc ttgccgggcg cgatgatatg
    12601 aagcacgttc aggagttctc tcttatattc gtgtatccgt tttacctgaa tgtcgaaaat
    12661 gcttgcaact cgtataccga tttgacaagc aagacatatt gtgatactta tttattagaa
    12721 gctgaattat tttccctgaa taccctggga gctacgttta gcgaagaaat agataaatct
    12781 atgctcaacc tctacaataa ttttcttttt acaaaatgct ttgtcgattt cttccaaaaa
    12841 atgcagcttg atggcataga ggccgcaagg aaattctggg attcatatcc ttacaagaac
    12901 atatatcccc cgaatgcatc ggatattttc gaaaaaatga ttgattttta tatcgatctc
    12961 agccacgtac cgaaagagca gtttgatgcg ctgataacaa ggtcttctcg gaaaagcagg
    13021 cgcggtttcg atggcttctt agacattctt tcccaacgca aagaagacac caatgccttc
    13081 ggctggggac cttcccgggc atatccccca ggtacccata tagtcaggca ggataccccc
    13141 gcagaggccg tctttcttgt ggaaagcgga ctcctgaagc tatcgcggat agtccacaca
    13201 gggagtgaag taatcgccgg tcttcgccgt
//
LOCUS       DATKOU010000163        11797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2916928, whole genome shotgun sequence.
ACCESSION   DATKOU010000163 DATKOU010000000
VERSION     DATKOU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916928"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1190
                     /gene="argC"
                     /locus_tag="VMU21_07525"
     CDS             150..1190
                     /gene="argC"
                     /locus_tag="VMU21_07525"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HUO77413.1"
                     /translation="MIKVAICGGSGYAGAELLRILAAHPHVEVTSVTSEKSPGKRVTD
                     LFPHLSRYKHLIYEVLDRERILPKADLFFMALPHAASQEAVDFFVARGKRVIDLSADY
                     RLKDAKTYEEWYKVPHRFPATLKKAVYGLPELYRKKIAKAHIIANPGCYPTGAVIGLY
                     PAVKKGFIHPDTLVIDSKSGTSGAGRQSDVIFSFCEVNEGFKAYSIGSHRHTPEIEQE
                     LSIAAGKEIRVNFTPHLVPLDRGILTTIYAKLVKSLEVKEIHDAYASFYRKEPFTEVL
                     PLGKFPNVKNVRGTNLCHVGIAVNKRTSTLVVVTSIDNLVKGASGQAVHNMNIIMGFD
                     EETALSAIALCP"
     gene            1150..1362
                     /locus_tag="VMU21_07530"
     CDS             1150..1362
                     /locus_tag="VMU21_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77414.1"
                     /translation="MKKPPSAQSPSVPDTKNFENPKAEEVRLRALYRNIVLRGDVRLP
                     IKAMKALIGPDCAFHLYRSSHRTKRD"
     gene            complement(1359..1586)
                     /locus_tag="VMU21_07535"
     CDS             complement(1359..1586)
                     /locus_tag="VMU21_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HUO77415.1"
                     /translation="MIERSRIEDALNQIRPALQRDGGNVELVDVTPEGVVKVKLVGAC
                     GTCPMSILTLKRGIEATLKQRVPEVASVESV"
     gene            complement(1736..2710)
                     /gene="hemB"
                     /locus_tag="VMU21_07540"
     CDS             complement(1736..2710)
                     /gene="hemB"
                     /locus_tag="VMU21_07540"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HUO77416.1"
                     /translation="MVFPINRPRRLRKNEVIRRMVRETSLSADDFIYPLFAIYGKGVK
                     KEIASMPGCFQESIDELVNTVREVHSLGIPSVMLFGIPEHKDERGSGAYDDKGIVQNA
                     IRAIKDAVPELYVITDVCLCEYTSHGHCGIIEHGDVANDPTLELLAREAVSHAEAGAD
                     MVAPSDMMDGRVEAIRLALDEEGYPETPIMSYAAKYASAFYGPFREAAESTPQFGDRR
                     SYQMDPANRREALKEVSLDIEEGADIVMVKPALSYLDIISDVRDNFDVPVAAYSVSGE
                     YSLIKAAAKLGWIDGERMMMEILTSMKRAGADLILTYFAKEAARILNR"
     gene            complement(2724..3044)
                     /locus_tag="VMU21_07545"
     CDS             complement(2724..3044)
                     /locus_tag="VMU21_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HUO77417.1"
                     /translation="MVTISELAAEKGKEILKAEGKAGWGLRIYTAGGGCCGPSYGMDI
                     DESPAEGDEVLETNGLKVFLDQNTVKSLSGMQIDFVDDGERQGFVLTGGKSAACDSGC
                     SSCG"
     gene            3357..4289
                     /locus_tag="VMU21_07550"
     CDS             3357..4289
                     /locus_tag="VMU21_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO77418.1"
                     /translation="MMQIHQLLFRAAYLGMSHRELWDRVSCAEGILKECTLCPRNCGV
                     DRTSGEVGFCKTGNKPFVSSWGPHFGEEKPLVGRFGSGTIFFGHCNLGCVFCQNWTIS
                     HLGEGEEISFARLAKIMGDLEGMGCHNINLVTPTHQMPMILHSLAIAVEKGLTIPIVY
                     NCGGYESLEALKLLRGIVDIYMPDFKYADSEMTLKYSKVKDYPERAKASLKEMHMQVG
                     DLVMDGRGIARRGLLVRHLVLPEDIAGTEQVVSFIAGEISENTYLNIMDQYSPCYKAS
                     EQPLLNRKITKEEYGSAVRKAQHKGIKRIDGVTG"
     gene            4335..5702
                     /locus_tag="VMU21_07555"
     CDS             4335..5702
                     /locus_tag="VMU21_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUO77419.1"
                     /translation="MFETILIIDNDTENTRRMIEALEARDYLVFTAPNRDAALTMVRK
                     VRPRLIFVNPVMPEGGGLELCSIIHEEEEFKEVPIVVLSTFEGATDPRYASLYGIVDS
                     LKTPCVPEELISKTEDVFLKQPAEAQSVVSEPGEHVEGEGIEIIEPRHKEVGKTDLSS
                     KEFGQEEETKPEGGEEVHEEVSVSDETAGQGKITEERLSAYAMKRSMRTRGTKNALLM
                     PVIGVAAVMILILAAILFYERDSSPDRKVQPPAATKPVQPSQEQTVNAGPAREPQKPE
                     QSGGQGAVPPMPKADIPSPAPGSEPKAAASKPETKVALPNPEPKAATPKPEAKGAAPK
                     PEVKATVPKQETKVAATKLEAKAAVPKPEPKASAMKSGAKPSAKGMYSAQMGAFKSEK
                     NAETLMKKYKEKGYEAFVYKTGTKDTGLFYRVLIGKFNDKKEALQMVKGISAKEGVQA
                     ILFRD"
     gene            5932..7041
                     /locus_tag="VMU21_07560"
     CDS             5932..7041
                     /locus_tag="VMU21_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HUO77420.1"
                     /translation="MCRLAAITSKEYFSPMENILALETMKEGHDGSGLGLTLKDLGDE
                     FKELKEYPIMSGICSKRGIGVLDEFMDKQGFKLNHVWTPRIRKLKGIAGRDYYFARVY
                     DYPEHFRDKSVKDKEDLLMNTRLAIRRIGEPDKSIFVFSFYPDVITLKEVGDPLQLGE
                     FFGLDKDGIKAKIIFAQGRQNTNYAIYLYACHPFFIQGFCSMTNGENTAFVPIREFLM
                     GRGFPGYMGYNSDSEVFTHILHYTVRQLGFPLHYYKDVITPLKTSEIEQRRDKDALRI
                     MKQSLRPLCIDGPNMVIGFTPDGTCFMVHDSKKLRPGVVGGAKGKFALMSEECGVDSA
                     VPKRDKQNDIFPMKYDMVIISPQAKEVRVWNQLHG"
     gene            7020..8681
                     /locus_tag="VMU21_07565"
     CDS             7020..8681
                     /locus_tag="VMU21_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HUO77421.1"
                     /translation="MEPAARLDNNHQLSLREFPYIIRWRDDRCKRCGRCTAVCPVKAI
                     EPAVKVQRFVQSEGPVPAPTAVRKIIQVVEQVTDMERYCTGCGTCTLVCPNEAIEPEY
                     NQQHKFYHYKNKGGEPYKRGGRRNDPSVSTLDRLKFTRISMLTDPALDAGRHEFRVRT
                     LLGRILPPEELPLSVADGKLVLNRKGGKFIPPVREIYPIMIGSMSVGALSPSMWEGLA
                     MGVAYLNEVEGMPVVMCSGEGGMPPRLLKSKYLKYFIIQIASGYFGWDEIIHALPHMV
                     EDPAAIEIKYGQGAKPGDGGLLMAQKVLKLIASIRGVPQFVDLSSPPTHQTKYSIEEA
                     VAKMIQSMSMAWGFRVPVYPKISGTKTARAVLNNLARNPYAAALSIDGEDGGTGAAYN
                     VSMDKMGHPIASNIRDCYLDLVKQGKQNELPLIAAGGIGKKGNLAANAAALIMLGASA
                     VSIGKYIMQAAADCFGDEYNRCNLCNTGKCPRGITTQDPKLYRRLDADKVAERVVEVF
                     KAADVELKKIFAPMGRSTELPIGMSDGLSIEDKAMAERLEINYAC"
     gene            8671..11082
                     /locus_tag="VMU21_07570"
     CDS             8671..11082
                     /locus_tag="VMU21_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO77422.1"
                     /translation="MRVKKEKKQGARGKVQGARKPQASSSVLTVYGDVKGKRVPSRVL
                     EEEIQHAVEEGARELHIIADGQHGIGGRIWPRSASGGGAVKITVEGPVGQRLGSMGMS
                     GTEILVRGSASDDIGWLNCGAKITVLGDVTNGAFNAAAQGILYVQGGGGARCDTMTKH
                     NPRFDPPQSWYFRGVGDSFAEFKAGGIAVVCGVDPRDPENILGYRPCVGMVGGTIYFR
                     GPIQGYSERDVKLLDLTPQDWEWLKTNMKPFLHAIDRMSHYKELTRSSDDWKKLMAYT
                     PQEKRARKWFKISTPEFRKNMWEKEVGQGGIFAEYLDHDLTLLPYITTGKERRNKPLW
                     SNEKYAPPCAYACPTQIPSHKRASLIRQGRLNDALELVLKYSPLPATVCGEICPNLCM
                     QSCTRAHLDKPLEIDMMGTLALDLPAPKKEAATGHTIAVVGGGPAGMSAAWQLALRGH
                     TVDIYESSGSLGGKLELCIPKERLPRKILQKEISRFRELGVNVHLNTTVDKAAFGEIY
                     KNHEIVVVACGAHEPRKIPFPGSKDIISAYDFLRDINLGNAPDLEGKKVVVIGAGNVG
                     MDVASEAYNHGAESVTAVDIQKPAAFGAEMEIAKSKGTQVVWPRLTERFDKNQGKLFF
                     KDGTLMDADFVVMSIGDVPKLDFLPPGIHTEKGWITVNDKYQTSDVKVYAIGDVTGLG
                     LVTHAIGHGRLTADYIHYELMHAPRFPEIKQAISYDKIKTEYYDVCRGDFVTPEVSAN
                     RCMSCATCRDCKMCEATCYWGAISRVEYKDGSYEYVVDDELCIGCGFCAGVCPCGVWE
                     MVENV"
     gene            complement(11107..11721)
                     /locus_tag="VMU21_07575"
     CDS             complement(11107..11721)
                     /locus_tag="VMU21_07575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77423.1"
                     /translation="MGIRDDLKNIIRELLGKTSSSIFLDKSLAILDESADNKESLMAA
                     ADRISNRIALFIDEELAKKVLEQLKAELGRSASPPGEKRRHVRITSSTKAYVTCNGSP
                     HEFYTENLSEGGMYIKTNTPFPIGTKLEITLHLDAGTQIHLKGVVIYIKRPFSDIFEH
                     PSGMAIEFREINDNDRRILRDFIKRASAQDIPENKEETKSKPAP"
BASE COUNT         3065 a         2841 c         3130 g         2761 t
ORIGIN      
        1 agaagggcgc acggaagaga ttccagttct ccaagagata acaattttgt gaccagtccc
       61 gcagtcggct gtctgcagtc tcaataaccc aaagactgag ttctcctgtc tgactccgga
      121 cagaggactg ggggcagagg gtaattcaaa tgataaaagt ggccatatgc ggcggaagcg
      181 ggtacgcagg ggcggaactc ctcaggatat tggcggcaca cccacacgtt gaagtaacct
      241 ctgtaacctc tgaaaaatca ccggggaaaa gggttaccga tctgtttcct catctttccc
      301 gatacaaaca tctcatctac gaggtattgg acagggaaag aatacttcca aaggcggacc
      361 ttttttttat ggcactcccc catgcagcat cccaggaggc ggttgatttt tttgtagcgc
      421 gggggaagag ggttatcgac ctatctgccg actacaggtt aaaggatgca aagacatatg
      481 aggagtggta taaggtccct cacaggtttc ccgcaacgct caagaaggca gtgtacggtc
      541 ttccggaact ctacaggaag aagatcgcga aggcacatat tatcgcaaat cccggctgtt
      601 atcccacagg agcggtaatc ggcctttacc cggcggtaaa aaaagggttc atccatcccg
      661 acactctggt aatcgactct aagtcaggca cctccggtgc ggggaggcag tcagatgtta
      721 ttttttcgtt ctgtgaagtg aacgaaggat tcaaggcata cagtattggc tcacacaggc
      781 atacccctga gatcgaacag gaactctcga ttgctgcagg caaagaaatc agggtaaatt
      841 ttactcccca ccttgttcct ctggacaggg gaatacttac gactatttac gcaaaactcg
      901 taaagtccct ggaggtaaaa gagattcacg acgcctatgc gtcgttctac aggaaggaac
      961 ctttcacaga agttcttccc ctgggaaagt tccccaatgt gaagaacgta agaggtacca
     1021 atctctgcca tgtaggcatt gcagtgaaca agagaacgag cacccttgtt gtggtcactt
     1081 ccatagacaa cctcgtaaag ggtgcatcgg gacaggcagt gcataatatg aacattatca
     1141 tgggttttga tgaagaaacc gccctcagcg caatcgccct ctgtccctga tacaaagaac
     1201 tttgagaatc caaaggccga ggaggtacgc ctcagggctc tctatcggaa cattgtcctt
     1261 aggggcgatg tcaggctgcc catcaaggcc atgaaggcat tgataggccc tgactgcgcg
     1321 ttccatcttt accggtcatc tcacaggaca aagagagatt aaacagactc cactgatgca
     1381 acctcgggca ctctttgttt caacgtcgct tctatgcctc gcttcaaggt taaaatagac
     1441 atggggcatg tgccgcaagc gcccaccagt ttcaccttta ccacaccttc aggtgttaca
     1501 tccacgagtt ccacgttccc gccgtctctc tgcaacgcag gccgtatctg attcaatgca
     1561 tcctcaatcc ttgatcgttc tatcattcct tatcacctcc cgcacgagta ttttctatta
     1621 tacccaaatc ctgagaaact ctccatgatt cacatcataa ccaataggct gaaagatctc
     1681 tgctgaattc ttggtgaggg agagggaacc agaaagggga ccgtattttg ctttactagc
     1741 ggttcagaat ccttgcggct tcttttgcaa agtaagtgag aatgaggtca gcccctgccc
     1801 ttttcatgga ggtgaggatt tccatcatca tacgctctcc gtcaatccat ccaagcttcg
     1861 ctgcagcctt tatcagggaa tattcaccgc tcacactata cgccgccacc ggaacatcaa
     1921 aattgtccct tacgtcagag atgatatcaa ggtaagaaag agcgggcttc accattacga
     1981 tgtctgcccc ttcctcgata tccagagaga cctccttcag ggcctctctc ctgttcgccg
     2041 ggtccatctg gtaagagcgc cggtcaccga actggggggt ggattcagcc gcctcgcgaa
     2101 aagggccata aaaagcagat gcatactttg ccgcatatga cattatgggt gtctcaggat
     2161 atccctcttc atccagggca aggcgaatcg cctcgactct gccatccatc atatctgagg
     2221 gtgcaaccat gtccgcacct gcctctgcgt gtgataccgc ttcccttgca aggagttcca
     2281 gtgtgggatc gttggccaca tcaccatgct ctattattcc gcagtggcca tggctcgtat
     2341 actcgcacag gcagacatcg gtgatcacat ataattcggg aactgcgtct tttattgccc
     2401 ttatggcgtt ctggactatt cccttgtcat cataagcccc tgagcctctt tcgtccttat
     2461 gctctgggat accgaagagc attaccgaag gtataccgag ggaatggacc tccctcactg
     2521 tgttcacgag ttcatctatt gactcctgaa aacatcctgg catggaggca atctcttttt
     2581 tcactccctt gccgtatatg gcaaaaaggg gatatatgaa gtcatcggca gagagtgagg
     2641 tctccctcac cattctcctg attacctcat ttttcctcag cctgcgaggt ctgttgatcg
     2701 gaaacaccat acctcctcta tgattacccg cagctgctac atccagagtc gcacgcggcg
     2761 gatttgccac ccgtgagcac aaacccctgt ctttccccgt catcaacgaa atcaatttgc
     2821 attccgctca ggctctttac ggtgttttgg tccaagaaga ccttgaggcc attcgtttca
     2881 agaacttcgt ctccttccgc agggctttca tcaatgtcca tgccatacga tggaccgcag
     2941 cacccgcccc ctgccgtata tatcctcagt ccccagccag cctttccctc tgccttcagt
     3001 atctccttgc ccttctctgc agccaattca gatatcgtga ccactgcaaa cctccttatg
     3061 attttccaaa gaccacatgg tttttggaag accttttctt ttaaggataa aggaaaggcg
     3121 gatgaaaaag caagaaaaca cgatgaggaa tccactctgc atgctgccaa aagaagagaa
     3181 tgcctcttca tttgaagacg gctgaagagt ctgttatgct tctacaagga gggcacagga
     3241 cataaaggct gaggtcagcg acgctgcaga ggaaattgtc atcatgtccg atgcaatgag
     3301 aaacatcgtt catatgctaa gggaatagcc ggaaaactta ccgggtgaga ggggagatga
     3361 tgcagataca tcaactgctg tttcgggcag cttatcttgg gatgtcgcac agggaactct
     3421 gggatcgggt atcttgtgcg gaggggatac tcaaggaatg tactctctgc ccgagaaatt
     3481 gcggggttga caggacctca ggtgaggtcg gcttctgcaa gaccgggaat aaaccttttg
     3541 tttccagctg gggaccccat ttcggtgaag aaaaaccttt ggtgggaagg ttcggatcag
     3601 gcaccatatt cttcggccat tgtaatctgg gatgtgtatt ttgccagaac tggaccataa
     3661 gccatctggg tgagggagaa gaaatttcct ttgcaaggct tgcgaagatc atgggagatc
     3721 ttgaggggat ggggtgccac aatataaatc tcgtgacccc cacacaccag atgcccatga
     3781 tcctccactc tcttgccatt gcggttgaaa agggtctcac tattcctatt gtgtacaatt
     3841 gcggaggata cgagtctctt gaagcactga agctcctcag gggaatcgtt gacatctata
     3901 tgcctgattt caagtatgcg gactctgaga tgacattgaa atattcgaaa gtcaaagact
     3961 atcccgaaag ggcaaaggct tcactaaaag aaatgcatat gcaggtgggg gacctcgtaa
     4021 tggatgggag ggggattgcc cggaggggtc tcctcgtgag gcaccttgtg ctacccgaag
     4081 atattgcggg gactgagcaa gtggtatctt ttattgctgg ggaaatctca gagaacacct
     4141 accttaacat tatggaccag tattccccat gctataaggc atccgaacaa cccctcctca
     4201 acaggaagat tacgaaggag gagtatgggt ctgctgtaag aaaagcgcag cacaaaggta
     4261 taaaaaggat tgacggtgtg acggggtaaa ggatatttga aatttttctt gaaagttgtt
     4321 aaagtgattc cataatgttt gaaacaatcc tcataatcga caatgatacc gagaacaccc
     4381 ggcgtatgat cgaagccctg gaagcgagag attatctggt gtttaccgct cccaatagag
     4441 atgcagccct caccatggta agaaaagtac gtccccggct gatattcgta aatcctgtca
     4501 tgcccgaagg cggggggctg gagctttgca gcataatcca cgaggaagaa gagtttaagg
     4561 aggttcccat tgtcgtgctc tccacctttg agggagccac agatcccagg tacgcttctc
     4621 tgtacgggat tgttgattcc ctcaagaccc cttgtgtccc cgaagagctt atctcaaaaa
     4681 ctgaggatgt ttttttgaag cagcctgctg aggcgcagtc tgtagtgagc gaacccggag
     4741 aacatgtcga aggggaaggt atagagatca tcgagcctcg ccataaggaa gtcgggaaga
     4801 cggacctctc ttcaaaggag ttcgggcagg aagaagagac aaagccagag gggggggagg
     4861 aagtgcatga agaagtatcg gtctctgacg aaacggcagg acaaggcaag ataaccgagg
     4921 agcgtctcag tgcatacgcc atgaaaagaa gcatgaggac acgaggcaca aaaaacgcat
     4981 tgcttatgcc tgtgattggt gttgccgctg ttatgattct cattttggca gcaattttat
     5041 tttatgaaag agactcttca cctgacagga aagttcaacc acctgctgcg actaaacctg
     5101 ttcagccttc tcaggaacag actgtgaatg ccggccctgc acgagagcct caaaaacctg
     5161 agcagtcagg gggacagggc gcagtgccgc caatgccgaa ggcagatata ccttctcccg
     5221 caccaggttc tgaacccaag gctgctgcgt ctaagccgga aacgaaagtt gctctgccta
     5281 accccgaacc caaggccgct acgcctaaac ctgaggcaaa gggtgccgca cctaaacctg
     5341 aagtaaaggc gactgtgccg aagcaagaaa cgaaagttgc tgcaacaaaa ctagaagcaa
     5401 aagctgcggt gcctaagccg gaacctaagg cctctgcaat gaagtccggg gcaaaaccgt
     5461 cagccaaggg gatgtattct gctcaaatgg gtgcctttaa gagcgagaag aatgccgaga
     5521 ccctcatgaa gaaatataag gaaaagggct atgaagcctt cgtttacaag acgggcacaa
     5581 aggacacagg actcttttat cgggtattaa tcggcaagtt taacgacaag aaagaggctc
     5641 tccagatggt caagggcatc agcgccaaag aaggcgtcca ggcaattctt tttagggact
     5701 gaccgaagag ccgactatct tttctgcggt caatgttttt atctatcaac cttcctcgat
     5761 ctccattttc cagcttttct ggcaagaagt tgacaaagga gcccagcaat tcttattata
     5821 agaagacggt gaacggttgc cgtttaatgg cgtcttatac cacaggggtg ggagagacgc
     5881 tttttttgcc tgaaaaggga tagcagatcg atcattcgtg aggttaagag aatgtgcaga
     5941 ctcgctgcca taacatcaaa ggaatatttc tctcccatgg agaatatcct tgccctggag
     6001 acaatgaagg aaggtcatga cggttccggt cttggtctca ccctcaagga cctgggcgat
     6061 gaatttaagg aactgaaaga atatcccatt atgtccggta tctgctccaa gaggggcatc
     6121 ggggttcttg atgagttcat ggacaagcag ggtttcaaac tcaaccatgt atggacgccg
     6181 aggataagaa aactgaaagg gatagccggc cgtgattact atttcgcgag ggtatacgac
     6241 tatcccgagc atttcagaga caaatctgtg aaggacaagg aagaccttct catgaacaca
     6301 cgtctcgcca tacgcaggat cggcgagccc gataagtcta tatttgtttt ctctttctat
     6361 ccggatgtga tcaccttgaa ggaggtggga gatccccttc agcttggtga gttcttcggg
     6421 cttgataagg acggcattaa ggcaaagatc atatttgcac agggaagaca aaacacaaat
     6481 tatgccatat acctttatgc ctgtcatccc tttttcatcc agggcttttg ttccatgacg
     6541 aacggagaaa acaccgcatt cgtacctata cgggaattcc tcatgggcag ggggttcccc
     6601 ggatatatgg gatacaatag tgacagtgaa gtcttcacgc acatcctcca ctacacggtg
     6661 agacagcttg gttttccgct ccattactat aaggatgtga tcactcccct caagacatca
     6721 gagatcgagc agagaagaga caaggatgcc ctgaggataa tgaaacagtc attaaggcct
     6781 ctgtgtatcg atgggccgaa catggtcatc gggttcactc ctgacggaac ctgttttatg
     6841 gtccacgatt ccaagaagct ccggcccggt gtcgtaggcg gggcgaaggg aaaatttgct
     6901 ctgatgtcag aggaatgcgg agtggacagt gcggttccga agagggataa gcaaaatgac
     6961 atttttccca tgaaatacga catggtgatc atctcgcccc aggcaaagga ggtgagggta
     7021 tggaaccagc tgcacggtta gacaataacc accagctctc tctgagggaa ttcccttata
     7081 tcatccgctg gagggatgac cggtgcaaga gatgcgggag atgcaccgca gtatgcccgg
     7141 taaaggccat cgagcctgcg gtgaaggttc agagatttgt tcagtcagag ggacctgtgc
     7201 ccgctcccac tgcagtgagg aagattatcc aggtagttga gcaggtgacc gacatggagc
     7261 gatactgtac gggctgtggg acctgcacct tggtatgccc caatgaggcc attgagccag
     7321 aatacaacca gcagcacaaa ttctatcatt acaagaataa agggggagag ccttacaaga
     7381 ggggcgggag aaggaatgac ccttcagtat ctacgcttga caggcttaaa tttacaagga
     7441 tctcgatgct cactgatccg gcccttgatg ccggaagaca tgaattccgt gtcagaacgc
     7501 tcctgggaag gatattaccc cctgaggagc tgcctttaag cgttgctgat ggcaagttgg
     7561 tgctgaacag gaagggcgga aaatttatcc ctcctgtgcg ggagatatac cccattatga
     7621 tcggcagtat gtcagtggga gccctttccc cttccatgtg ggaaggtctt gccatgggag
     7681 tagcatacct taatgaagta gagggaatgc ctgtggtgat gtgctcggga gagggcggta
     7741 tgccaccgag gcttttgaaa tcaaagtatc tcaagtactt tatcatacag atcgcttcag
     7801 ggtatttcgg gtgggatgag atcatccatg cgctcccgca catggtggag gaccctgctg
     7861 ccatagagat taagtacggc cagggtgcga aaccaggtga cggagggctt ctcatggccc
     7921 aaaaggtcct gaagctcatc gcgagcatca gaggggttcc ccagtttgtg gacctctcct
     7981 caccgcctac tcaccagaca aagtattcca tagaggaagc ggtggcaaaa atgatccagt
     8041 caatgtctat ggcatggggc ttcagggtgc ctgtgtatcc caagatttca gggacaaaaa
     8101 ccgcacgggc agtattgaat aacctcgcga ggaaccctta tgccgcggca ctctccattg
     8161 acggtgaaga cggcgggacg ggtgcggcat acaacgtatc catggacaaa atggggcatc
     8221 ccatcgcttc aaatatcagg gactgctatc tcgacctcgt caaacagggg aagcagaatg
     8281 aactccccct catcgcggca gggggtatcg gcaaaaaggg gaatcttgct gccaatgcag
     8341 cggcccttat tatgctcggc gcatccgcgg tttccatcgg caagtatatc atgcaggctg
     8401 cggcagactg ctttggagac gaatataacc gctgcaatct ctgtaataca gggaaatgtc
     8461 caagaggaat taccacacaa gatccgaagc tctaccggag attggatgct gataaggtgg
     8521 cggaacgggt ggtggaggtc ttcaaggccg ctgatgtgga gttgaagaag atattcgcgc
     8581 ctatgggcag gagcacagag cttcccatcg gcatgtctga tggattgagc atagaggaca
     8641 aggcaatggc agagcggtta gagataaact atgcgtgtta agaaagagaa gaagcaggga
     8701 gcaaggggca aagtgcaggg ggcaagaaag ccccaggctt caagttccgt gctgactgta
     8761 tacggcgatg tgaagggtaa aagggtccct tcccgcgtcc tggaagagga gatccagcat
     8821 gcggttgagg aaggggcgag ggaacttcat atcattgccg atgggcagca tgggatcggg
     8881 ggccgaatct ggccgcggtc cgcctcaggc ggaggggcag tgaagatcac ggttgaaggt
     8941 cctgtgggcc agaggctggg aagcatgggc atgtccggca ccgagatcct tgtcaggggc
     9001 agcgcttccg atgatatcgg ctggctgaac tgtggcgcga agattactgt ccttggtgac
     9061 gtaaccaatg gtgcatttaa tgcagcggct cagggaatcc tctatgtgca gggaggaggg
     9121 ggagccaggt gcgacaccat gacaaaacat aatccacgct ttgatcctcc ccagtcatgg
     9181 tatttcagag gcgtgggcga ttcctttgcc gaattcaaag caggcgggat cgcggtcgtc
     9241 tgcggggtgg accccaggga ccctgagaat attctcggct accggccctg cgtcgggatg
     9301 gtggggggga ccatatattt caggggaccc atccagggat acagcgagag agatgtgaag
     9361 ctcctcgacc tcacgcccca ggactgggaa tggctcaaga cgaacatgaa accattcctc
     9421 catgccattg acaggatgtc ccattacaag gaactcacgc gctcttctga tgactggaaa
     9481 aagctcatgg catacacccc tcaggaaaag agagcgagga agtggttcaa gatttctacc
     9541 ccggagttca ggaagaacat gtgggaaaaa gaggtgggac agggcggcat ctttgcagaa
     9601 tatctggacc atgaccttac acttttgccc tatattacta ccgggaaaga gaggaggaat
     9661 aagccccttt ggtcaaacga gaaatatgct ccgccctgtg cgtacgcatg cccgacgcaa
     9721 attccctctc acaagagggc ttccctcatc aggcaaggca gactgaatga tgcattggag
     9781 ttggtattga agtacagtcc cctgcctgca acagtgtgtg gggagatatg tcccaacctt
     9841 tgtatgcaga gctgtaccag ggctcacctc gataaacccc tggaaataga catgatggga
     9901 acattggccc tcgacctgcc cgctcccaag aaggaagctg ccacaggaca taccatcgca
     9961 gtggtgggtg gtggccctgc gggtatgagc gctgcatggc agcttgccct aaggggacat
    10021 acggttgata tttatgaatc ctccggttcc cttgggggaa aattggagct atgcatcccg
    10081 aaggaacgcc tcccccgcaa gatccttcaa aaggagattt cacggttcag ggagcttgga
    10141 gtgaatgtgc atttgaacac aacggtggac aaggcagcat ttggagagat atataaaaac
    10201 catgagatcg ttgtggtggc ctgcggcgcc catgagccga gaaagatacc cttccccggt
    10261 tctaaagaca ttatctcagc ctatgacttt ttgagggaca tcaacctggg caatgcccct
    10321 gacctagagg ggaagaaggt ggtggtcatc ggagcaggca atgtgggaat ggatgttgcc
    10381 tccgaggcat ataaccacgg cgcagaatcc gtgacggctg ttgacatcca gaaaccggct
    10441 gcctttggcg cagagatgga gatcgcgaag tcaaagggga cccaggtggt atggcccagg
    10501 ctgaccgagc ggtttgacaa aaaccagggg aaacttttct ttaaggacgg gaccttaatg
    10561 gacgcggatt ttgtggtcat gtccattggt gatgtgccga agctggattt cctgccgccg
    10621 ggcatccaca cagagaaggg ctggatcacg gtaaacgata agtatcagac ctctgatgta
    10681 aaggtgtatg ccattggtga tgtgaccggt ctcgggctcg tgacacatgc gatcggccat
    10741 ggaagactta cggcggatta tatccactat gaactcatgc atgcgccgcg ttttcccgaa
    10801 atcaagcagg cgatatccta tgataagatc aagactgagt attacgatgt ctgccgggga
    10861 gatttcgtca ctcccgaggt ttcggcaaac cggtgtatgt cctgcgccac ctgccgtgac
    10921 tgcaagatgt gcgaggccac ctgttactgg ggcgcaatca gcagggtcga gtacaaggac
    10981 ggctcgtatg agtatgtggt ggatgatgag ctttgcatag gctgcggctt ctgcgcgggc
    11041 gtctgtccct gcggcgtatg ggagatggtc gaaaacgtct agggccattc atcttcatta
    11101 gttgtcttaa ggagcaggtt tagatttcgt ttcttctttg ttctcaggaa tgtcctgagc
    11161 agatgctctc tttataaagt cccttaatat cctgcggtca ttgtcgttaa tttccctgaa
    11221 ctctatcgcc atgccggacg ggtgctcaaa aatgtcgctg aagggacgtt ttatataaat
    11281 aacgacacct ttcaaatgaa tttgagtacc ggcatccaag tgtaatgtta tctccagttt
    11341 tgtacctata gggaaaggcg tattggtctt aatgtacatt ccaccttcag agaggttttc
    11401 agtgtaaaat tcgtgcggtg acccgttgca tgttacatat gctttcgtgg aagaggtaat
    11461 tcttacatgt ctcctttttt ctccgggagg tgaggcactt ctcccaagct ccgccttcag
    11521 ctgttccaaa acctttttcg ccaactcttc atcaatgaat aatgcaatcc tgttgctgat
    11581 cctatcggca gcagccatga gactctcttt attgtctgct gattcgtcca ggatagctaa
    11641 agacttgtcc aggaaaatag agctggaggt ctttcccaga agttctcgaa taatgttctt
    11701 taaatcgtcc ctgattccca tgttacaatc ctacctcttt tattatcctg tagaagagat
    11761 tgatcacctt cacgataaaa atggcccctg ccaggga
//
LOCUS       DATKOU010000164         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2917322, whole genome shotgun sequence.
ACCESSION   DATKOU010000164 DATKOU010000000
VERSION     DATKOU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917322"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VMU21_07580"
     CDS             <1..311
                     /locus_tag="VMU21_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546020.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HUO77424.1"
                     /translation="GEDGNFEGKKARIEEFDKLLPGILRLNPDVLIITGDHSTPARMK
                     GHSWHPVPLLLKSPYVLGGLSSSFSERECVKGELGIFPAIHIMPLALANAGRLKKFGA
                     "
     gene            308..1657
                     /locus_tag="VMU21_07585"
     CDS             308..1657
                     /locus_tag="VMU21_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUO77425.1"
                     /translation="MIDTHCHLDMFDDSDAVIRRAGDAGIEYLITIGSDFESNLKNLV
                     IALNNDPVYASVGIHPHDAKDFSDETYAKIREWTKEKKVIAIGETGLDYHYDHSPRDV
                     QRDVFKKHLDLARETGLPVLIHSREARNDTLEILEKSGISKGVFHCFSGDLEMAEKVM
                     EMGFSISLAGPVTFKNAKELVEVAREIPDDYLLVETDAPYLAPVPLRGKTNEPCYMVH
                     TVRRLAELRGVSPEDIERITTLNAKRLFKIGKLVSRGEIAYKIRDSLYLNLTNRCTNS
                     CSFCVRFHKDYVKGHNLRLSDEPTEEELKEAIGDPSHYREVVFCGYGEPLLRLDIVKS
                     VARWVKEKGGHVRINTNGHGNLIHGRNILTELTGIVDTLSISLDAQDNETYQRICAPT
                     YKNAFQEVVSFIREAKDYVPSVQATVVETAGVDIEKCRKLTEELGVPLRVRKLDVVG"
     gene            complement(1860..2240)
                     /locus_tag="VMU21_07590"
     CDS             complement(1860..2240)
                     /locus_tag="VMU21_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77426.1"
                     /translation="MEEKRKHERFIIEGKDSHCKMLVSTELRLLNISISGAALSVSEK
                     LNMGWEYTLELESEERVVSVSGVVVWAKMTELQKNDRGEMVPRYEVGMRFKDVFNYKG
                     HELTDFIESKVFEKKFKVRLKLGC"
     gene            2612..2746
                     /locus_tag="VMU21_07595"
     CDS             2612..2746
                     /locus_tag="VMU21_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77427.1"
                     /translation="MTFYCGIDLHAKKSHICIIDKDGKKVKEESEEPTGGIGQTVIPN
                     "
BASE COUNT          737 a          664 c          694 g          695 t
ORIGIN      
        1 ttggggagga cggtaatttt gaagggaaaa aggcaaggat cgaggagttt gacaaactcc
       61 ttcccggaat ccttcgtctg aatccggatg tccttatcat taccggcgac cattccaccc
      121 ctgcgcgcat gaagggccat agctggcacc cggtgccact cctcttgaaa tcgccttatg
      181 tgctcggagg attgtcctct tcgttctctg agcgggagtg tgtaaagggt gagttaggca
      241 ttttccccgc catccacata atgcccctgg ccctggcaaa tgcgggaaga ctgaagaagt
      301 tcggtgcatg atcgataccc actgccatct cgatatgttt gatgacagcg acgccgttat
      361 ccgcagggca ggggacgcgg gcatcgagta cctcataacc atcggctctg atttcgagag
      421 caacctcaaa aatcttgtta tcgccctcaa taatgaccct gtgtatgcct ctgtgggcat
      481 ccatccccat gacgcaaagg attttagtga tgagacctat gcgaagatac gggaatggac
      541 aaaagagaaa aaagtgattg ccatcggcga gacaggactt gactaccact atgaccattc
      601 tccccgggat gtgcaaaggg atgtttttaa gaaacatctt gatctcgcac gcgagacggg
      661 gcttcctgtt ctgatccata gccgtgaggc aagaaacgat acactggaga tactggaaaa
      721 gtcgggaatc agcaagggag tattccactg tttctccggt gacctcgaaa tggccgagaa
      781 ggtaatggaa atgggcttct ctatctccct cgcagggccg gtgaccttta agaacgcaaa
      841 agaacttgtg gaggtggcaa gggagattcc tgatgattat ctccttgtcg agacagatgc
      901 cccctatctg gcacccgtgc ctctaagggg gaagacaaac gaaccttgct atatggtcca
      961 tacggtgaga agattggctg agctgagggg agtcagtccc gaagatatcg aaaggattac
     1021 cacactcaat gccaagaggc tcttcaagat cgggaaactc gtatcgaggg gtgagattgc
     1081 gtataagata agggacagtc tctaccttaa tctcaccaac agatgcacca acagctgctc
     1141 cttttgcgtc cggttccata aggattatgt gaaagggcat aacctcagac tttctgatga
     1201 acccacggaa gaagagctga aagaggcgat aggggaccct tcgcactacc gtgaggtcgt
     1261 cttttgcgga tacggcgaac cgctcctgag gctggatatt gtgaaaagcg ttgcccgatg
     1321 ggtaaaggaa aagggcgggc atgtgaggat caataccaac ggccacggga atctcattca
     1381 tgggaggaat atcctcacag aacttaccgg tattgtggac accctttcca tcagtctcga
     1441 tgcccaggac aacgagacct accagaggat ctgcgccccc acttacaaga atgcgtttca
     1501 ggaagtcgtt tcctttatac gggaggcgaa ggactatgta ccctctgtgc aggccactgt
     1561 ggtggagacg gcgggagtgg atatagagaa atgcagaaaa ttgacggagg aattgggagt
     1621 tccgctcagg gtaaggaagc tcgatgtggt tggctgattc atgccctgag agttcgcttt
     1681 gaaataaaca gccctttctt gcgaagtccg ttgacaacat gaaagcctaa ccgcgccgca
     1741 ggtatcttca cttctcatag taactctcac cggcaccctt tttccgttgc caaggagttc
     1801 gtcccgtact ttgcaaggct acgacgcacc gaagatacaa acttacattg agcgggtcct
     1861 tagcacccta gtttcaaacg caccttgaac tttttctcga agactttgga ttcgataaaa
     1921 tcggtgagct catgtccttt gtagttgaac acgtctttaa aacgcattcc tacttcatat
     1981 cgcgggacca tttcaccccg gtcattcttt tggagttcag tcatttttgc ccacacaacc
     2041 acaccagaga ccgaaacaac tctctcttcg gactcgagtt cgagcgtgta ttcccatccc
     2101 atgttgagtt tttcacttac gctgagcgct gcgccgctaa tacttatgtt caggagcctg
     2161 agctcggtag aaacgagcat cttgcaatgg ctgtcctttc cctcaatgat gaacctctcg
     2221 tgtttccttt tctcttccat ggtgaattac tcgtccattc ctttttacga aatctcctta
     2281 tgcctcgaac tacaagctcc ttttcagcat accagaatta tggcggaaag ataagcttct
     2341 gttagagttc actacatatt agagaaaaag aggggtttgt aatcatggat aatgccgttg
     2401 tctttgagga attgcaatgg tgtcctctta cctaaagcac gatgcggtct tatgcagtta
     2461 taaatatgct tccagcggaa aagtcgaggt ttctttcgga acttcacaat agtttcatct
     2521 aaacaaaagt gtttgataat tattgagagc gacctccgtt ggatgtgaca attgttccca
     2581 ttcaacaaat catcactcaa aggaggccgc aatgacattt tactgtggaa tcgatttgca
     2641 tgccaagaag tcccatatct gcattattga caaagatggg aaaaaggtaa aagaggagag
     2701 cgaagaaccg acgggtggaa ttggtcaaac agtgatacca aactgaaacg gctaactgac
     2761 acaatatcgg gcatcagtgg gtttgcgccg
//
LOCUS       DATKOU010000165         4471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_996399, whole genome shotgun sequence.
ACCESSION   DATKOU010000165 DATKOU010000000
VERSION     DATKOU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996399"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..440)
                     /locus_tag="VMU21_07600"
     CDS             complement(84..440)
                     /locus_tag="VMU21_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77428.1"
                     /translation="MRKLDHERRRQKRFVKRCTIAFTSDGQTYRGICRNLSLDGLFIK
                     TRKPLALEKTIEMIVHLPDGSTSKLTGRVTRATKEPYSLILERAGIPSKDGMGIQLIE
                     KDANYLKFLESLITRP"
     gene            complement(475..1449)
                     /locus_tag="VMU21_07605"
     CDS             complement(475..1449)
                     /locus_tag="VMU21_07605"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HUO77429.1"
                     /translation="MQIKKQMADFFRVDKELIIIFLLVAIAGFVFFFVSNQRAFLNFF
                     YFPVLIGAYYFGKRYATHAALLSILLIFIIAYFYPATFVSSSDNQLFRWLDIVTWGGF
                     LLTMGYCMGLLYEKKEKAGREIKSTYQGIIEMLSLLIDSVDKHTQSHSYRVSRVAERI
                     AREMRCPEGEVENIRIAALLHDLGKLGISAEILNKIGTLSVDERETMKNHTRFGADVL
                     APVGGRVLQILPYVLLHHEKYDGSGYHSIAGEGIPLGARIIAVADYYDALLSDRPYRQ
                     GLSPLNARKDIIANAGTHFDPAVIKAFDAVFDKLDTDMPLMPDSLFSH"
     gene            complement(1500..1835)
                     /locus_tag="VMU21_07610"
     CDS             complement(1500..1835)
                     /locus_tag="VMU21_07610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77430.1"
                     /translation="MIKGRYGGPEKRRSPRLAKRFIATIEYEEKVHEIRTIDISEHGV
                     LIPKRIPPPVGTLVKVCLTIRDETSSFEGVVKRHTKCLFNGVETVGIGIEFSSPEYQE
                     FVKDKILIT"
     gene            2372..2641
                     /gene="rpsO"
                     /locus_tag="VMU21_07615"
     CDS             2372..2641
                     /gene="rpsO"
                     /locus_tag="VMU21_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HUO77431.1"
                     /translation="MSLEKEKKQSIIGTYKTHEKDTGSPEVQIAILSERITYLTEHFK
                     THKNDHHSRRGLLKLVSQRRSLLDYLKAKNKGRYEKVVERLGLRR"
     gene            2655..>4471
                     /gene="pnp"
                     /locus_tag="VMU21_07620"
     CDS             2655..>4471
                     /gene="pnp"
                     /locus_tag="VMU21_07620"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HUO77432.1"
                     /translation="MKKVEIEVRGKLLTIETGEIAKQSDGAVVVKYGDTVLLATAVAN
                     KKMREGLDFFPLTMDYQEKAFAAGKIPGGYFKREGRPTEKEILTSRLIDRPMRPLFPE
                     GFYCETQGIVSVLSYGEENVSDVLGIIGMSAALTISDIPFSGPVAAVKVGLLNGELIV
                     NPDLIESENMELNIVVAGTEDAVIMVEGGASEVSEKILLDAIEYGHSEIKRIVAVQKE
                     LRGIAGKPKRPLVEKPVDVELQDSVKAYVIDRIKEHIAIPGKQRRQEALDMLLEDALN
                     HFNAEEKGLTKEIANIFFDIEKELVRKMIINEGIRADGRRPDEIRQITSRVGMLPRAH
                     GSALFVRGETQALVAVTLGTADDEQKIDSLEGEIYKAFMLHYNFPPFSVGEVKPLRSP
                     GRREIGHGALAEKSLKTVIPAKEVFPYTIRIVSDILESNGSSSMATVCGATLALMDAG
                     VPITAPVAGIAMGMVQEGDKTVVLTDILGLEDHLGDMDFKVTGTEAGITAFQMDLKTG
                     GVSKGVMEKALEQARQGRVFIIGKLKEALSAPRESLAPHAPRIYTMQIKQDKIRDVIG
                     TGGKVIRGIIEQTGVKIDINDSGIINIASSDETSASKAM"
BASE COUNT         1263 a          985 c         1089 g         1134 t
ORIGIN      
        1 ccttctgatt tggttctgag ctctctctgc attctatggc aggatttact cggactgttc
       61 cccattgggg tgctttatca tgcttaaggc ctggtaatca atgattccag aaattttagg
      121 taattggcat ctttttctat cagttgaatg cccatcccat cttttgaagg tattcccgct
      181 ctttccagga tcaaactata aggctctttt gtcgctcttg tgactctgcc ggtcaatttt
      241 gatgtcgagc catcgggcag atggactatc atttcaattg tcttctccag ggcgagcggt
      301 tttcttgtct ttatgaaaag gccatccaaa gacaaatttc tacatattcc tctataagtc
      361 tgaccatccg aggtaaatgc aatggtgcaa cgctttacaa atcttttttg ccttctcctt
      421 tcatgatcca atttcctcat tgtggtaatc cttgagccgc tcattaatct ctttctaatg
      481 tgaaaataat gaatcaggca ttaagggcat gtcagtatct agtttatcga aaacagcgtc
      541 aaaagccttt atcacagcgg gatcaaagtg tgtgccggcg tttgcaatga tatcttttct
      601 cgcattgagg ggtgaaagac cttgacgata tggccggtct gacaataagg catcatagta
      661 atccgcaact gcgatgattc ttgctcctag cggaatgccc tcacctgcta tgctatggta
      721 tccacttcca tcatattttt catgatgaag cagaacatag ggcagaatct ggagaaccct
      781 gcctccaaca ggggcgagca catctgcgcc gaatctcgtg tgatttttca tcgtctcccg
      841 ttcgtcgaca gacaatgttc ctatcttgtt aaggatttcg gcgctgatac caagtttccc
      901 caggtcgtga agcagggccg caatacgaat attttccact tctccttcgg gacatctcat
      961 ttctctcgct atcctttcag cgacacggga gactctgtag gagtggctct gggtgtgttt
     1021 gtccacggaa tcgatgagga gagacaacat ttcgattatt ccctggtacg ttgattttat
     1081 ttctctgcct gccttctctt tcttctcata caggagtccc atgcaatacc ccatggtaag
     1141 aagaaatccg ccccatgtga caatgtctaa ccatctgaat aactggttat cggaagaaga
     1201 aacaaaagtt gcgggataaa aataagcaat tatgaaaatc aatagaatgg acagtagtgc
     1261 cgcgtgtgta gcataccgct tgccgaaata ataggcaccg ataagaacag gaaaataaaa
     1321 gaaattaaga aacgcacgtt gatttgagac aaaaaagaaa acaaatcctg caatagcaac
     1381 gagaagaaaa attattatta actctttatc caccctgaaa aaatccgcca tctgtttctt
     1441 aatctgcata acttacctcc aatttacaaa gaagcaattg agacctccta ccagtaaact
     1501 taggtgatca aaatcttatc cttaacaaat tcttgatact cgggtgaaga aaactcgatc
     1561 cctatgccaa ccgtttctac tccgttaaag agacactttg tatgtctttt gacgaccccc
     1621 tcaaatgagg atgtttcatc cctgattgtc agacatactt taacgagagt ccctacaggt
     1681 ggaggtatgc gttttgggat aaggacacca tgttccgaaa tatcaatggt tctaatttca
     1741 tggactttct cttcatattc tatcgttgcg ataaacctct tcgcaagtct cggactcctt
     1801 cttttttctg gaccgccata tcgaccttta atcataatta tttgccctta ttaaggattc
     1861 actatctcaa aatgtaatca taaagtctac tggccaagaa aagctttctg gctattttgt
     1921 tatattcgta cattatttgt ctcttgtctt ttacctgaca agtagcaaat accataccta
     1981 ctgtaagtcc aggatgagag gatgtctgaa aaaaacaaaa tagctcagtc ctgcaaagac
     2041 ttacaaattt cacacgagat ttatagatgc acaggcaaga ataaagcata gcttacaagc
     2101 tgtcctgaaa caagacgatg tgtccggaat ttgcaagata accatgctac tgtttgaatt
     2161 tcccatgagt cgcgagttat gctgaaaccc tctgattgaa cgattactgg gttctgcaag
     2221 catgaagtat tcccctttta gccattggtg ataaagagtg cgggcgggaa atgttattag
     2281 ggcagacaca ggggaaaaac ggaattctca acaacaaatc ggggagatat ggtatagtat
     2341 aggaccaaaa aatatgaaag gtggtgattt gatgtctttg gagaaggaga aaaagcagtc
     2401 cataataggc acctataaga cccacgaaaa agacacgggg tcacccgagg tgcagatcgc
     2461 gatcctgagc gaacggataa cctacctcac agagcatttc aagacacaca agaatgatca
     2521 tcattcacgg cgcggacttc tcaaactggt gagtcagcgg aggagcctcc tggattatct
     2581 caaggcgaag aacaaggggc gatatgagaa ggttgtggaa cggttaggat taaggagata
     2641 ggagaaattc attgatgaag aaagtggaga tagaggtcag gggaaaactg ttaaccatag
     2701 aaacgggtga gattgccaag cagtctgacg gcgccgttgt ggtaaagtat ggcgatacag
     2761 ttttgctcgc caccgcagtt gcgaacaaga agatgagaga gggtctggat ttttttccgc
     2821 tgaccatgga ctaccaggaa aaggcatttg ccgcaggaaa gatccctggt ggatatttta
     2881 agagggaagg gaggcccacc gagaaggaaa tactcacctc ccgtctcatc gaccggccca
     2941 tgaggcccct tttccctgaa gggttttatt gcgagacaca ggggatcgtc tccgtgcttt
     3001 cctacggcga ggagaatgta tctgatgtcc tgggaatcat agggatgtcc gcagcattga
     3061 ccatttctga tatccccttt tccggccccg ttgcggcggt gaaggtcgga ctgttgaacg
     3121 gtgagttgat cgtcaaccct gatctcatag agtcagaaaa catggaactc aatatcgtgg
     3181 ttgccggcac cgaggatgcg gtgatcatgg tagagggggg cgcatcagag gtatcagaga
     3241 agattctttt ggatgccatt gaatatggac attctgagat caagaggatt gttgcggtcc
     3301 agaaagagtt gaggggcatt gcaggaaagc caaagagacc ccttgtggaa aagcctgtgg
     3361 atgtggagct gcaggacagc gtcaaggcgt atgtgatcga ccggattaag gaacacatcg
     3421 ccattccggg caagcagaga agacaggagg cccttgatat gcttctggaa gatgcattga
     3481 accacttcaa tgcagaagaa aaagggctga ccaaggaaat tgcaaatata ttctttgaca
     3541 tagagaaaga gctcgtcagg aagatgatca tcaatgaggg cataagggca gacggcagga
     3601 ggcctgacga gatacgacag atcacctcga gggtcggtat gctcccaagg gcgcacggtt
     3661 ctgccctttt tgtaaggggc gaaacccagg ccctcgttgc ggtgaccctc gggactgctg
     3721 acgatgagca gaagatagat tccctggaag gagagatata taaggccttt atgctccatt
     3781 ataacttccc tcccttcagc gtgggagagg tcaagccatt gcgctcgccc gggcgaaggg
     3841 agatagggca cggggccctt gcggagaaat ccctgaaaac agtgattccc gccaaggagg
     3901 tatttcccta taccataagg attgtctctg acattcttga atccaacggt tcttcatcca
     3961 tggcaacggt gtgcggcgct accctcgcac tcatggacgc tggtgtgccc atcacagccc
     4021 ctgttgcggg cattgccatg ggtatggtcc aggaagggga caaaactgtt gtgctcaccg
     4081 atatcctggg acttgaggac cacctgggcg atatggactt caaagtgacc ggcaccgagg
     4141 ccgggatcac tgcattccag atggatctca agaccggcgg agtgagcaag ggagtgatgg
     4201 aaaaggccct tgaacaggca agacagggga gggtgttcat tataggcaag ctgaaagaag
     4261 cactctccgc gccaagggaa agtctcgccc cccatgcacc aaggatatac accatgcaga
     4321 taaaacagga caagataagg gatgtcatcg gaaccggggg gaaagtaatc cggggaataa
     4381 tagagcagac aggagtgaag atagacatca atgattcagg gataatcaat atcgcctcat
     4441 ccgatgaaac ttctgcttca aaggccatgg a
//
LOCUS       DATKOU010000166         5315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2152661, whole genome shotgun sequence.
ACCESSION   DATKOU010000166 DATKOU010000000
VERSION     DATKOU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152661"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..473)
                     /locus_tag="VMU21_07625"
     CDS             complement(285..473)
                     /locus_tag="VMU21_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77433.1"
                     /translation="MELNTMSYNKCICRKCGTQYATMLNSDAELQNEPCPKCGEKELK
                     LSGPVGLNELRSMFGGGG"
     gene            complement(618..1013)
                     /locus_tag="VMU21_07630"
     CDS             complement(618..1013)
                     /locus_tag="VMU21_07630"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUO77434.1"
                     /translation="MRDATQLFKLLSDETRMRILMLLAHRELCVCQLMGILGVSQPLI
                     SRNLSLLSSAGLLNERREGKLVFYSVKKNSTPMARKILELLRTELKDTARLREDLRSL
                     SDCHEFQIKTGKCDMKTFLSFMEKQRTRK"
     gene            complement(1088..1957)
                     /locus_tag="VMU21_07635"
     CDS             complement(1088..1957)
                     /locus_tag="VMU21_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUO77435.1"
                     /translation="MKEGELGKVYASGETIFNEGSTGEVMYVIQSGKVKITKRTDSGE
                     MTIATLGEGEIFGEMAVFDRMPRSATAIALGDARVLSVDKKKLFTTINRDPTLVFKML
                     ESMSQRIRKLDGELAKLKKEHTEMSRICVDVDETSTLILKEAKHVIPADNGSLMLIDN
                     EGKSLVIAAAFGEEADPKVILSVGEGIAGDVLRTGRAELVNNVSLDSRFIPGGSPITS
                     LLCAPLRCRNYNFGVINMSNHSEKPFTLDDLKMLDALALYASIAIQNAKNFSHLSDAA
                     SEVLRHATMLHMS"
     gene            complement(1954..3297)
                     /locus_tag="VMU21_07640"
     CDS             complement(1954..3297)
                     /locus_tag="VMU21_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77436.1"
                     /translation="MTQTADNLDAYVLEDGAHIGVVGGGPTGSFFSIFALKMARMIGR
                     QIQLTIFEPKDFTKDGPAGCNRCGGIISELLVQALAVEGINLPDSVVRKGINSYNLHT
                     NHGGVYFATPDLERTIATVYRGGGPKGMIQEGKESFDYFLLKDAIKEGATLDPLRVDR
                     IEIRDKKPVLFSKDRELGQFDLVVGAFGVNSTTPKMFEEIGFGYKEPPTVTTAIAELL
                     LGEENVQKHFGNSVHLFLLPDKGMKFAAMIPKGAYVTLCILGKEMHANTVSEFLEKPV
                     VKNVLKDIPYTVGCRCLPKMNVGAPLKPFTDRVVTCGDAGSTRLFKDGLGAAYLMGKA
                     AAKTALFQGVSASHFQEDYYPVYHSIIIDNRYGQFLFTVTDVYRKNKILTEGMLRVVR
                     REQQGPADRRILSSILWNMFTGNERYKNIFPKALDIRMHGNFLREFSQVLTRRAS"
     gene            complement(3409..4863)
                     /locus_tag="VMU21_07645"
     CDS             complement(3409..4863)
                     /locus_tag="VMU21_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77437.1"
                     /translation="MADGITLHILGDYGPFSRMGKSIGYQVTIGQSSFLIDCGAPLFQ
                     QIGGHRLKGIKGLIITHCHDDHKRWFTDLALFNMYAPDFPNRVYLLTSEDVHDELMRA
                     SASALDKSLSKDSKTIIDIPYDEYVDYQMLGPRAKYRIVSLDEGNGKASLFVADRNGN
                     VLGPDRAKIVLNYKTKRPRLLFKDPHYGEWIEPESFYPFSSPVFYEENKNTLKDEEGF
                     TIEALKAPVWHGVTGIGIKIRTAAETLVFSSDTNHNIELWKQLYEEKRDQRLPVSREE
                     FGSSSILYGDINDYIERIWSEERYREAVNAFRDAVVIHDISFGNSVVHTNYEALNKTS
                     LSKDSVVLTHSPDRITSEWVLCRAEKTFRIKGNTFSEKVGNRMYPMNADVYHKEAGRY
                     YVGYKNEHGRYVVYEKSGILRIVFEEESEVPSSGKPLYRVDLYEDISGKYFPKIERSD
                     CIYLQRKDGRVELIEFHDKGSRGSIVENRRHRLL"
     gene            5019..>5315
                     /locus_tag="VMU21_07650"
     CDS             5019..>5315
                     /locus_tag="VMU21_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HUO77438.1"
                     /translation="MKDPRHIKIDISEAGNRLDTFLAGKTGITRSQIQKLIENGDVLV
                     NGAAVSQNYRTKGNVVVSLTIPFEETEGLVPEPIPIEILYKDDAVVVVNKPAGMV"
BASE COUNT         1235 a         1375 c         1297 g         1408 t
ORIGIN      
        1 gagacttttg gtgtagcagc taatctgccg ctccatgaac gcgagacgca ttatatactg
       61 gaagtaacct tgagatagtt tcggctcttg agaactgtcg cttcgtcggt gaagtccaca
      121 tcaaaggcgg attaggtttc aatggggaag ggtgtcaagt ttagagcgaa atctgcagat
      181 agagagggta ttaaaagaca gccgacaaac tcttgatcga aacaagagat gtcgactgcc
      241 caggattttg agagacctga ccggtcctag caactgtagg ggtttcagcc accgcctccg
      301 aacatgctgc gaagctcgtt gagaccgact ggtcctgaaa gtttcagttc tttttctccg
      361 cacttgggac atggctcatt ctgcagttcc gcatcgctgt tcagcatggt tgcatactgg
      421 gttccgcact ttctgcagat gcatttgttg tatgacatgg tgttcaactc catcactaac
      481 ctccgtcgtt ctctattgtt tcattgtttc ttaattataa cataacaaac ccggcatggc
      541 aatcccctgt gctttacgga tgctgagggg ctttttctcc gcgattaagg cggcgggcaa
      601 gaagggcaag ggattcgtca tttccttgtc cgctgttttt ccatgaacga gagaaatgtc
      661 ttcatgtcgc attttcctgt ttttatctgg aactcatggc agtcgctcag agagcgtaag
      721 tcctctctta accttgcagt atctttcagt tctgttctta atagttcgag aattttccgc
      781 gccatcgggg tggagttttt cttcactgag taaaacacca gctttccctc ccttcgctca
      841 ttaaggagcc ccgcactgct gaggagggag aggttccttg aaatcagagg ctgggaaact
      901 cccaggatcc ccatgagctg gcagacacag agctccctgt gggcaaggag cataagaatc
      961 ctcatccttg tttcatcaga caacagttta aaaagctgtg tggcatctct catatagata
     1021 tatttatata tgattatcta atatttgtca agagaaaaag cggctaggga gaaaaagcga
     1081 tgatgcgtca ggacatgtgg agcatggtcg catgtctgag cacttcgctt gccgcgtcac
     1141 tgaggtgcga gaagttcttt gcattctgaa tcgcgatgga cgcatagagt gcgagggcat
     1201 ccagcatctt gaggtcatca agggtgaagg gtttttcgga gtggttactc atgttgatca
     1261 cgccgaaatt gtaattcctg cacctgaggg gagcgcagag cagggaggtg atgggggagc
     1321 cgccgggaat aaaacgtgag tcaagagaga cattgtttac cagctctgcc ctgcctgtcc
     1381 tcagcacatc gcctgcgatt ccttcaccca cagagaggat caccttaggg tcagcctcct
     1441 caccaaaggc tgcggcaatg acgagggatt ttccctcatt gtcaatgagc atgagggaac
     1501 cgttatcagc agggatgaca tgcttcgcct ccttcaatat aagcgtggag gtctcgtcaa
     1561 catcgacaca gatgcgcgac atttcggtgt gctccttttt gagcttcgca agctccccat
     1621 caagtttcct tatcctctgg ctcatggatt ccagcatctt gaatacaagg gtagggtccc
     1681 tgttgatggt ggtgaagagc tttttcttgt ctacgctcag taccctcgca tcacccagtg
     1741 caatggcagt ggcagagcgg ggcatacggt cgaaaactgc catttccccg aagatttccc
     1801 cttccccgag ggtcgcaatg gtcatctccc ccgaatcagt cctttttgtg atcttcacct
     1861 tacccgactg aattacgtac atcacctcac ctgtgctgcc ctcgttgaaa atagtctcac
     1921 cacttgcgta caccttcccc agttcgccct ctttcatgat gccctcctcg tgagaacctg
     1981 ggaaaattcc ctcaagaaat tcccatgcat cctgatgtca agggccttgg ggaatatatt
     2041 cttgtatctc tcgttcccgg taaacatatt ccagaggatt gagctgagga tcctgcggtc
     2101 agcgggaccc tgttgctctc tccggacgac cctgagcatg ccctcagtga ggatcttgtt
     2161 tttccggtac acgtccgtaa cggtgaagag gaactggcca taacggttgt cgatgatgat
     2221 actgtggtaa accgggtaat aatcctcctg aaaatggctg gcgctcaccc cctgaaagag
     2281 cgcggttttg gcagcagcct ttcccatgag atatgcagcc ccgagtccgt ccttaaagag
     2341 ccttgtggag cccgcatcac cgcatgtgac taccctgtcg gtgaagggtt tcaggggcgc
     2401 ccctacgttc atcttcggca aacagcggca tcccacggtg tacgggatgt cctttaacac
     2461 gtttttcact accggttttt ccagaaattc actcacagtg tttgcgtgca tctctttacc
     2521 aaggatacag agggtgacat acgcgccctt gggaatcatg gcagcaaact tcatcccctt
     2581 gtccgggagg aggaagagat ggaccgaatt tccaaaatgc ttttgaacgt tctcttcgcc
     2641 cagaagcagc tctgcgatgg ctgtggtgac cgtgggaggt tccttgtatc caaaaccgat
     2701 ctcttcaaac atctttgggg tggtgctgtt aactccaaat gcccccacca ccaggtcaaa
     2761 ctgccccagt tcccggtcct tcgagaagag aacaggcttc ttgtccctga tctcaatgcg
     2821 gtcaaccctg agcggatcaa gcgtagcccc ctccttgatc gcgtccttga gcagaaaata
     2881 atcaaaactc tccttcccct cctggatcat tcctttcggc ccgccgcccc ggtacactgt
     2941 ggcgatggtc ctttcaaggt caggagttgc gaagtacaca ccgccgtggt ttgtgtggag
     3001 attgtaggaa ttaatgccct ttctcaccac agagtcaggg agattgatgc cctcaacagc
     3061 aagggcttgg acaaggagtt ctgatattat ccccccgcag cggttgcacc ccgcaggtcc
     3121 gtccttggtg aagtctttag gctcgaagat cgtgagctgg atctgtcttc ctatcatcct
     3181 tgccatcttg agcgcaaaga tgctgaaaaa ggaccctgtg gggccgcccc ctaccacccc
     3241 gatatgtgcg ccgtcttcta acacatacgc atcaaggtta tcggctgttt gcgtcactgg
     3301 accccttgta cgaataataa tgggatatgc gcgctactct atctcctaag gctattacag
     3361 tctcccctgc gtgtcaacac agatcttgtc ttcagcctgc gcgtggactt agaggagcct
     3421 gtggcgacgg ttctcaacga tgctccccct gctccccttg tcatgaaact cgatcaattc
     3481 aaccctgccg tcctttcgct gcagatatat gcagtcgcta cgctctatct ttgggaaata
     3541 cttccctgat atgtcttcat agaggtcaac acgataaagg ggctttcccg aggagggcac
     3601 ctctgattct tcttcgaaaa cgatcctgag aatgccggat ttttcataca caacgtacct
     3661 tccgtgctca ttcttatatc cgacatagta tctccccgct tccttatggt atacgtccgc
     3721 attcatggga tacattctgt tccccacctt ctcagaaaag gtgttccctt tgatcctgaa
     3781 ggtcttttct gccctgcata atacccattc cgaggttatc ctgtcaggac tgtgggtcaa
     3841 tacaaccgaa tccttgctga gcgaagtctt gttcagagcc tcgtagttgg tatggactac
     3901 gctgttgcca aaagaaatat catggataac aacagcatca cgaaaggcat tgaccgcttc
     3961 cctgtaccgc tcctcactcc atatcctttc tatatagtca ttgatatcgc catagagaat
     4021 ggaagaagac ccgaattcct ctcttgagac tggcagtctc tggtccctct tttcttcata
     4081 gagctgcttc cacagctcta tgttatggtt agtatcagaa ctgaagacga gggtctctgc
     4141 ggctgtcctg atctttatcc ctatccctgt aacgccatgc cataccggcg ccttgagtgc
     4201 ttctatggtg aagccttcct catccttaag ggtgtttttg ttttcttcgt agaagacggg
     4261 agaggaaaag ggataaaaac tctctggctc gatccattcc ccgtaatgtg ggtccttgaa
     4321 gagaagcctt ggcctttttg tcttgtagtt caatacaatc ttcgccctgt cggggcccag
     4381 gacatttccg ttccggtctg caacaaagag gcttgccttc ccgttccctt cgtccagtga
     4441 gacaatcctg tacttcgcac gcggccccag catctgataa tccacatact catcataggg
     4501 gatatcaata atggtcttgg aatcctttga gaggcttttg tccaacgcag aggcggatgc
     4561 cctcatgagt tcatcatgca cgtcctcaga ggtaaggaga tagaccctgt tgggaaaatc
     4621 aggagcgtac atattaaaaa gggcgaggtc agtgaaccat ctcttatggt catcgtggca
     4681 gtgcgtgatg atgaggccct tgattccctt gagtctgtgg ccccctatct gctggaagag
     4741 cggcgccccg caatcaatga gaaagctgga ttgtcctatg gtcacctggt acccgatgct
     4801 tttccccatc ctggaaaacg ggccgtagtc accaaggata tgtagggtaa tcccatcagc
     4861 cattttgacc tattctagca taaagggtga gcaggttaaa gatgttttca tacgaacagg
     4921 ggagaatggg aaggcctgcc ttgaggtata atgtaccggt aagcttcgcc ttcccatttt
     4981 actgggaagg aacagggtga ttactgtata acaaacagat gaaggacccc aggcacataa
     5041 agattgacat ctcagaggca gggaaccggc ttgatacctt tcttgccgga aaaaccggga
     5101 ttacgcgctc gcagatacag aagctcattg aaaatggaga tgtcttggta aatggagccg
     5161 ctgtcagcca gaactacagg acaaagggca atgtggtggt aagcctgacc ataccctttg
     5221 aagaaacaga aggtcttgtc cctgaaccga tacccatcga aatactttat aaggatgatg
     5281 cggttgtggt ggtgaacaaa cccgcgggta tggtg
//
LOCUS       DATKOU010000167         5298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2154403, whole genome shotgun sequence.
ACCESSION   DATKOU010000167 DATKOU010000000
VERSION     DATKOU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154403"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VMU21_07655"
     CDS             <1..558
                     /locus_tag="VMU21_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HUO77439.1"
                     /translation="EVKAESDICCTSANVVKVIESLPSERIICIPDRNLSMWAQKNTK
                     KQVIAWDGFCHVHDRVTKEDVLKAKRQHPKALLMAHPECRPEVLELANHVTSTSGMLR
                     FAKASDEEEFIVGTELGLMHRLKKDNPHKLFHPLRKDMICPNMKKTTLGSVARALTGM
                     THRISVPEEIRIPAKRALDRMLAIK"
     gene            659..1816
                     /locus_tag="VMU21_07660"
     CDS             659..1816
                     /locus_tag="VMU21_07660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUO77440.1"
                     /translation="MNILRDKIIKKIQSEGPISFETFMDMALYEPSLGYYMRDSAMIG
                     RAGDFYTSPHLHSIFGAMLGRQMEEMWEWMGRPPVFFVVEMGAGMGYLAKDLMASLTE
                     REIFPHLEYRIIEQNPSIKAHQERLLEDFRYKVKWASDLKQMGPVSGCFLSNELLDAF
                     PVKLVEMDHELMEIYVALSSQYLLSKENEEALLENLTFVEVKKLCSAETGDYFKGFSL
                     ELPKGYRTEVNLRVKEWLLKVNERLAEGFVLTIDYGYTAMDYYSEDRNRGTLLCYHRH
                     QVSEDPYRNIGQQDMTAHVNFSSLKKWGDELGLKTVGFSHQGAYLVSLGIGEVMNELY
                     GGCPDVFDVAKIKGLILPQGMGDSHKVMIQYKGKGEPELKGFTLRNQGEKL"
     gene            complement(1791..2567)
                     /locus_tag="VMU21_07665"
     CDS             complement(1791..2567)
                     /locus_tag="VMU21_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HUO77441.1"
                     /translation="MNKVHVHVPYPKIKEYLTYITQKKLNLEIFFPSSSLDSLKKEDI
                     RILMERLDYNPSLSIHAPFMDMSPGAVDSRIRDVTVERFLQVMEIAELLSPQIVVFHS
                     GYEKWKYAHRVDIWLEASLTTWRPLRERAAALNLKIAIENIFEDEPSNLLLLMEELHS
                     EHFGLCFDTGHFNLFTRLPLEEWIKSLKPYIFEVHLHDNDKSRDQHLPIGEGTFNFAA
                     LFSELQGNHCVYTIETHTPERVLKSMERLQEYITASPPGS"
     gene            complement(2564..2863)
                     /locus_tag="VMU21_07670"
     CDS             complement(2564..2863)
                     /locus_tag="VMU21_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HUO77442.1"
                     /translation="MPIYEYQCSGCGKKHEVVQRHNDKPLTTCPDCGGPMTKLISSTS
                     FVLKGTGWYKTDYASGGTKKTAEGDKEKPAKEEKKADTPSESKATSALQTTSKTE"
     gene            2978..3574
                     /gene="coaE"
                     /locus_tag="VMU21_07675"
     CDS             2978..3574
                     /gene="coaE"
                     /locus_tag="VMU21_07675"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HUO77443.1"
                     /translation="MLIVALTGNYGMGKTTALEMFRDLGAATLKTDEVVDTLLKDAGV
                     LKTIKREFGDGVFSGDGDLNREKLSSIVFRDSGMRGRLEKILHPLVFERIEEFLKGLV
                     NKVAGKGVVIIEIPLLFEKGYTERFSKNIVVYADEEAALKRLEELGITRDNAMMRLNA
                     QMPIKEKVKRADFVIDNSGSLEHTRAQVIETYHKLLED"
     gene            3582..4529
                     /locus_tag="VMU21_07680"
     CDS             3582..4529
                     /locus_tag="VMU21_07680"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HUO77444.1"
                     /translation="MIIVRGLENLKEKYRDVALTIGNFDGVHIGHQKILKKVTERARV
                     MAGTAMAVTFDPHPAKVVTPEKGVRILTSFQEKVRLMELHGIEVILCIHFDKEFAGIR
                     PDEFIRDVIVGRIGAREVIVGHNYSFGKGKKGTTGLLRSRSRKYGFIFRVIRSARLYG
                     DVVSSSRIRSLLSRGRVCEASWLLGRPYMMEGTVVKGTGRGGRLLTIPTANISTLNEL
                     VPKEGVYAVKVDLEGKILDGAANLGENPTFGENRVSHEIHILDFSEDVLHKKLKVHFI
                     DRIRDEKTFPDVHALREQIKRDIERAREILKTKKYPTLR"
     gene            complement(4516..>5298)
                     /locus_tag="VMU21_07685"
     CDS             complement(4516..>5298)
                     /locus_tag="VMU21_07685"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77445.1"
                     /translation="YHKDKGLLKCHYCGHTRNAADICGRCRSSRFEMIGAGTQRIAAE
                     IEHHLHVRPLLLDKDVLKEDPVHRDLHHISLGEKMMVGTKVITKRLPRRKSFGLCAFL
                     NPDMQLHLPDFRSAELLFQEIISTSEYVEDAGLLLIQTRIPEHYVFRYVKTYNLNSFY
                     KEELSVRRSLSYPPFSRMVLLTITAKGEIEEMLMKALADTGGTVEIVGPVRTSGKGSG
                     QWKLLLKSASKEYLHNYARSILKSLEKRRGIRITADVDPISM"
BASE COUNT         1417 a         1115 c         1426 g         1340 t
ORIGIN      
        1 gaggtaaagg cggagagcga tatctgctgc acatctgcca atgtggtaaa ggtgatagaa
       61 tctttgccgt ctgaacggat catctgcatt ccggacagaa atctctccat gtgggcgcaa
      121 aagaacacga agaagcaggt aattgcgtgg gatggttttt gtcatgtcca tgacagggtc
      181 acaaaggaag atgtgctgaa ggcgaagaga cagcatccga aagccctttt gatggcgcac
      241 ccggaatgca ggcctgaggt gcttgaactg gcgaaccatg tgacgagcac gtcagggatg
      301 ctgaggtttg caaaggcatc agacgaagag gagttcattg tcgggacaga gcttgggctt
      361 atgcaccgac tgaaaaagga caatccccat aagctgtttc atcccctaag aaaagacatg
      421 atatgtccaa atatgaaaaa aaccacgttg ggaagcgtgg caagggcgct cacagggatg
      481 acgcatcgga taagtgttcc tgaggagata cggattcctg ccaaaagggc gctggacaga
      541 atgcttgcga taaagtgagt gtcgtttctt cttttggcat ctcagtcttg gccagaaatc
      601 caggttttta cttaaaagag ccccttgagc gaggaatgat gttcctttgg cgattctcat
      661 gaatatcctc agagacaaaa tcatcaaaaa aatacaatca gaaggcccca tcagcttcga
      721 gacatttatg gatatggcac tctatgaacc ctcccttggc tattatatga gggattctgc
      781 catgataggc agagcagggg acttctatac cagcccccat ctccactcca tatttggtgc
      841 aatgctgggc aggcagatgg aggagatgtg ggaatggatg ggcagaccgc cggtattttt
      901 cgtggtagag atgggagctg gtatggggta ccttgccaaa gatctcatgg cctctctcac
      961 tgaaagggaa atatttcccc atctcgaata ccgcattatt gaacagaacc cttcaataaa
     1021 ggcacatcag gagagattac tcgaggactt tcggtacaag gtgaagtggg cttctgattt
     1081 gaagcaaatg ggaccggttt cgggctgttt tctctccaac gaacttctag atgcctttcc
     1141 tgtaaagttg gtggaaatgg atcatgagtt gatggagatt tatgtcgcgc tctcttccca
     1201 atatctttta tcaaaggaaa acgaagaggc tcttttagag aacctcacat ttgttgaagt
     1261 caaaaaactc tgcagcgcag aaaccgggga ttattttaag gggttttccc tggaacttcc
     1321 caaggggtat aggacagagg tgaatctgag agtcaaagaa tggctcctga aagtaaacga
     1381 gagacttgcg gagggttttg tactgaccat agactatggg tatacggcga tggactacta
     1441 cagtgaggac agaaacaggg ggacgttgct ttgttaccat cgccaccagg tgagcgagga
     1501 cccttacagg aacatagggc aacaggacat gactgcgcat gtgaatttct cttccctgaa
     1561 aaagtgggga gatgaactgg gattaaagac cgtgggtttc agtcaccagg gggcttacct
     1621 ggtttctctg gggatcggtg aagtgatgaa cgagctgtat ggcggatgtc cggacgtgtt
     1681 tgatgtggca aagattaagg gtctcatcct ccctcagggt atgggtgatt cccacaaagt
     1741 gatgatccag tataaaggga aaggcgaacc ggaattaaag ggatttaccc tcaggaacca
     1801 gggggagaag ctgtaatgta ttcctgcaat ctctccatgc ttttcaagac ccgttctggt
     1861 gtgtgtgttt caattgtgta gacacaatga ttcccctgca attcagagaa aagggcagca
     1921 aagttgaagg tcccctctcc aatgggcaga tgctggtcac gacttttgtc attgtcatgg
     1981 aggtggactt caaaaatata gggtttgagg cttttgatcc attcctccag agggagcctt
     2041 gtgaagaggt taaagtgtcc tgtatcaaaa cataacccaa agtgttctga gtgaagctcc
     2101 tccataagca gaaggaggtt cgacggttca tcctcgaata tgttttcgat ggctatctta
     2161 agatttaagg ctgctgccct ttccctcaga ggtctccatg tggtgaggct cgcctcaagc
     2221 cagatatcca ccctgtgtgc atacttccac ttttcatagc ccgaatgaaa tacgacaatc
     2281 tggggtgaaa ggagttcagc gatctccatt acctggagaa atcgttcaac tgtgacgtcc
     2341 ctaatcctcg aatccaccgc accgggagac atatccataa atggtgcatg gatggacagg
     2401 gaaggattat aatcgagacg ttccattaat atcctgatat cttctttttt gagggaatcc
     2461 agggaggatg aggggaaaaa tatctccaga ttcagcttct tctgcgtgat ataggtgaga
     2521 tattccttta ttttgggata gggaacatgg acatggactt tgttcactct gtcttcgaag
     2581 tggtctgtaa ggcagacgtt gccttgctct ctgagggagt gtctgctttc ttctcctctt
     2641 tggctggctt ttccttatcg ccttccgcgg tcttttttgt gccaccagag gcgtaatcgg
     2701 tcttatacca gcccgttcct ttcaggacga aggaggtact tgaaatgagt tttgtcatgg
     2761 gtccaccaca atcaggacat gtggtgagag gcttatcatt gtgcctctgc accacttcat
     2821 gctttttgcc acaccctgag cactgatatt cgtaaatcgg catacacgga cctccgtatc
     2881 acacacttgt acaataataa cttattcaaa acagcatggt caaatcagcc gagcccaaga
     2941 cttatgccct tttatatttt ctgaggtata ataaccagtg ctcatcgtcg cgttgacagg
     3001 aaattacgga atgggaaaga ccactgcctt ggagatgttc agggatctcg gtgcagccac
     3061 tctgaagact gacgaagtgg tagatacatt gctcaaggat gcaggggtcc tgaagacgat
     3121 caagagagag tttggagacg gggtattttc aggtgacggg gatctcaaca gggaaaagct
     3181 ttcttccatt gtcttcaggg acagcggcat gaggggcaga cttgagaaga ttctccaccc
     3241 ccttgtcttc gaaagaatcg aagagttcct gaaaggtctg gtcaataagg tggctggaaa
     3301 aggtgtggtg attatcgaaa ttcccctgtt gttcgagaag ggttataccg aaaggttctc
     3361 taagaatatc gtggtgtatg cggatgagga agcggcccta aaacgtcttg aggagttggg
     3421 cattaccagg gataatgcca tgatgcggct gaacgcacag atgcccatca aggagaaggt
     3481 taaaagggca gactttgtga ttgacaacag tggttctttg gaacatacga gggcacaggt
     3541 aattgaaacc taccacaaac ttctggaaga ctagggagag aatgattatc gtaagagggc
     3601 tggagaacct caaggaaaag tatagggatg tggcactcac catagggaat tttgacggtg
     3661 tgcatatagg tcaccagaag atactcaaga aagttaccga aagggcgcgg gtcatggcag
     3721 gaacagccat ggctgtaacg ttcgatccac atcctgcaaa ggttgtcacg ccggaaaaag
     3781 gggtgaggat tttgacatcc tttcaggaaa aggtgaggct gatggaactc cacggcatag
     3841 aggtaattct gtgtatccac tttgacaagg agtttgcagg cataagaccg gatgagttta
     3901 tacgtgatgt gattgtgggt aggatcgggg caagggaggt aatcgtcggc cacaattatt
     3961 ccttcggaaa agggaaaaag ggtaccacag gacttttgag aagcaggagc aggaaatacg
     4021 gattcatctt cagggtcatc aggagcgcga ggctctatgg tgatgtggtg agcagcagca
     4081 ggatcagaag ccttctgagc cgggggaggg tctgtgaggc atcatggctc ctgggaaggc
     4141 cttatatgat ggaggggact gtggtaaagg gcacgggaag gggagggagg cttcttacta
     4201 ttcccactgc caatataagt actctcaatg agctcgtgcc aaaggaaggc gtgtatgcgg
     4261 tgaaggtcga cctggagggg aaaatactcg acggcgcggc aaacttaggg gaaaacccca
     4321 ctttcggaga aaacagagtg agccatgaaa tacacattct tgatttttcc gaagatgtcc
     4381 ttcacaaaaa actcaaggta catttcatcg accgcatcag ggatgagaag acgtttcctg
     4441 atgtccatgc cctccgggaa cagatcaaga gagatataga acgtgcgagg gaaatactga
     4501 aaacgaaaaa atatcctaca ttgagatagg atcaacgtca gccgttatcc ttattcccct
     4561 tcttttttca aggcttttca gaatgcttct cgcatagttg tggaggtatt ctttagaggc
     4621 agattttaac aaaagcttcc attgacctga gcctttcccg gaggtcctca ccgggccgac
     4681 tatctccacc gtccctcctg tatcggccag ggctttcatg agcatttcct ctatctcccc
     4741 tttcgcggta atggtcaaaa gcaccatgcg ggaaaagggc ggataggaga gggatctcct
     4801 cacggaaagt tcttccttat aaaaactgtt gaggttgtaa gtttttacat atctgaacac
     4861 atagtgctcc ggtatccttg tctggataag gagcagacca gcatcctcga cgtactctga
     4921 tgtgctgata atctcctgga agagcagttc ggcggacctg aaatcaggaa ggtgaagctg
     4981 catgtcaggg ttgaggaaag cgcaaagacc gaaggatttc cttctgggga gtctctttgt
     5041 aatcactttt gttcctacca tcattttttc cccaagggag atatggtgga ggtctctgtg
     5101 gacgggatcc tctttcagca catccttatc caaaagcagt ggtcttacgt gaagatggtg
     5161 ttcgatttct gccgcgattc tctgtgtgcc ggcgccgatc atctcgaacc gtgaactcct
     5221 acacctcccg catatatctg ctgcgttacg ggtatgcccg caatagtgac atttgagaag
     5281 gcctttgtct ttatggta
//
LOCUS       DATKOU010000168         4892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4081984, whole genome shotgun sequence.
ACCESSION   DATKOU010000168 DATKOU010000000
VERSION     DATKOU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4081984"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VMU21_07690"
     CDS             <1..1247
                     /locus_tag="VMU21_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HUO77446.1"
                     /translation="ELRQQVQQKDSEIQQVRKELQNEGQLKTEALTSLRESQRSFEEQ
                     KALIDAMKKEMTDTFNALSSAALKSSSEDFLRLASEHLGKVVSDTKGKLGEHQAAMDG
                     MIKPLQETLKRYEEQIRSIEEGRHKAYGSLEEQLRALASTHENLQRETSNLVSALRKP
                     QVRGRWGEMQLRRVAELSGMSMHCDFTEQLSVDTEKGRIRPDMVVHLPMEREIVVDSK
                     VSLEAYLDSISASTEDERKTKMEKHAQQVRVHMIKLASKEYWSQFKQSPEFVVLFIPG
                     EAFLSAALESDTSLIEDGIEKRVIIATPTTFIALLRAIAYGWRQEQLTKNAQEISELG
                     RQLYERIGTLVQHFESIGSNLEKAIGAYNKAVGSIEARVLPSVRKFRELGVTAAEEIS
                     VLEQIDQTPRNLNLLPEESQEA"
     gene            1271..1549
                     /locus_tag="VMU21_07695"
     CDS             1271..1549
                     /locus_tag="VMU21_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77447.1"
                     /translation="MSEKMSHEEFVKKAIVSLRKEGYKGIHTVYSGFNEAFKKYFSGE
                     NPVEVTNGLAQEGKIVIRPVKGGVMLYLSEDAPSGKSSADEALKKMGL"
     gene            1602..2285
                     /gene="nadD"
                     /locus_tag="VMU21_07700"
     CDS             1602..2285
                     /gene="nadD"
                     /locus_tag="VMU21_07700"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HUO77448.1"
                     /translation="MTPQNKKKVAVMGGAFNPIHYGHLRAAEEVRIKLGFEKVLFIPS
                     GNPPLKMKDLADANHRYEMTRLSIETNPFFDISDIECRGSRKSYTVETLTALGERHPE
                     NEFFLILGNDSFLDIPAWYQPERLTELANFVVVSRPGFRFSGLSPEIPIAPETLFALD
                     ARALEMHRLRLEGGRELLLLNITPMDISATAVRTLVKAGKSIKYLLPEQVESYIILHK
                     LYREGSDHL"
     gene            2303..2662
                     /gene="rsfS"
                     /locus_tag="VMU21_07705"
     CDS             2303..2662
                     /gene="rsfS"
                     /locus_tag="VMU21_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HUO77449.1"
                     /translation="MAAATSALAKKAKDTLILDLRGLVVFTDYFVICSGESTTQVKAI
                     AEHIEAEFMKNSLKPLSIEGSSYAHWILIDYGDVVVHVFQNETRDYYELEKLWLDAPR
                     IPLERENTSLTGERYHF"
     gene            2662..3978
                     /locus_tag="VMU21_07710"
     CDS             2662..3978
                     /locus_tag="VMU21_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77450.1"
                     /translation="MGKLAVLLFVVILVALGYLAVLNKEPITFALAPKTVYEMPKIAL
                     ILLSSAVGAAVMLLIFFIRDTKRFIEGRQYQRKQKKDMIVQELYSKALNAILADNEEE
                     ARSALESVLKEEPRHMDALLRLGDIAAGDEDHQKAMNYYRTAGEIQPHSLEVLFSLER
                     IMGKTGRTTEALMYLDKILELDPDNLAALYRKRTLLERKEKWDDLISLQRAIIKCEHN
                     EKDREREQINLLGYKYEQGRYHLENGELEKAKKAFKTILRMDMNFVPAYLGLAEAMLR
                     EEDTEDAIAFLEKGFAQTSSPIILARIEDLLINLGEPGRLIMLYKNSILKEPKNQVLR
                     FFMGKLFYRLEMLDDAFDTLIAVDGTSAPYPELHQLLGNIYLRRQQCERAVEEFKKVI
                     DIKKPFLLPYCCNSCGYASLDWSGRCPNCREWNTYEFNLHGICRTQ"
     gene            3991..4653
                     /locus_tag="VMU21_07715"
     CDS             3991..4653
                     /locus_tag="VMU21_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase partner protein"
                     /protein_id="HUO77451.1"
                     /translation="MNTHHSEESPCDPLPVMPHTAPHRVVLDTSLFVNPDARTSLGNT
                     PTEALETFLFLAAQIHILEFYMPPSIFEELLHFVEKDKIPGDLLVILHQKPPKRHELK
                     CPAVLLYELIDDMRERVNKGLRIAEKAVRGAEKNKADEMIQDLRRKYREALREGIIDS
                     KEDVDLLLLAMELDALLITADQGLIRWAEKLGIQWLFPEKFKDYLMGAIKRTELISGQ
                     RE"
     gene            complement(4683..>4892)
                     /locus_tag="VMU21_07720"
     CDS             complement(4683..>4892)
                     /locus_tag="VMU21_07720"
                     /inference="COORDINATES: protein motif:HMM:NF027524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77452.1"
                     /translation="YDEYLDFLVEFIQRLSPEIVLQRLFAMAPDDILIAPKWERTRQE
                     IIRNIKERFELRNAVQGSHCLCYRK"
BASE COUNT         1394 a         1097 c         1292 g         1109 t
ORIGIN      
        1 atgaattaag gcagcaggtt cagcagaaag actctgagat acagcaggtg cggaaagaac
       61 tccagaatga aggacaactg aagaccgagg ccctcaccag cctcagagaa tcccagagga
      121 gctttgagga acagaaagcc ctcattgatg ccatgaagaa agagatgacc gacaccttta
      181 atgccctttc ctcggcggca ctgaagagca gcagtgagga tttcctgagg cttgcctccg
      241 agcacctcgg caaagtggta tcggacacca aagggaaact gggagagcat caggcggcca
      301 tggacgggat gataaaacct ctccaggaga cactcaagag atatgaagaa cagatacggt
      361 ccattgagga aggcaggcat aaggcctacg gaagtctcga agaacaactg agagcgctgg
      421 catcaaccca tgaaaatctc cagagagaga cgagcaatct tgtgtctgca ttgcggaaac
      481 cacaggtaag gggtcgatgg ggtgaaatgc aattaaggcg tgttgctgaa ctctcaggca
      541 tgtctatgca ttgcgatttt accgagcaac tgtccgtaga tactgaaaag ggaaggatta
      601 gacctgatat ggtcgtacat cttcccatgg agagagagat tgttgttgat tcaaaagtgt
      661 cgctcgaagc gtatcttgac tcaatctccg cgtcgacgga ggacgaaaga aaaacaaaaa
      721 tggagaagca cgcgcagcag gtacgggtgc atatgataaa actcgcgtca aaggaatact
      781 ggagccagtt taaacagtca ccagaattcg ttgtgctttt tattccaggg gaagccttcc
      841 tgagtgcagc ccttgaaagc gatacttccc ttatcgagga tggcatagag aagcgggtta
      901 tcattgcaac ccctactacc tttattgctc tgctcagggc tatagcgtat ggatggcggc
      961 aagagcagct cacaaaaaat gcccaggaaa taagcgagtt aggccgccaa ttatatgaaa
     1021 gaatagggac gttggttcaa catttcgaga gtatcggttc caatctcgaa aaagccatcg
     1081 gggcatacaa taaggctgtg ggctccatcg aagcacgggt attgccttct gtgaggaagt
     1141 ttagagaact cggggtgact gctgcagaag agatttctgt tttggaacaa atagaccaga
     1201 caccaagaaa tctgaattta ttgcctgagg aatctcagga agcataaata aaccgtagaa
     1261 ggagggcact atgtctgaga aaatgagtca tgaggagttt gtgaagaagg cgattgtgag
     1321 tttgaggaag gaagggtaca agggcatcca taccgtctat tcgggcttta atgaggcgtt
     1381 caagaagtat ttttccggtg aaaaccctgt ggaggtgacc aatggtctgg cacaggaggg
     1441 gaagatcgtg ataaggccgg ttaagggtgg agttatgctc tacctctctg aagacgcccc
     1501 gtcgggcaag agctcagcgg atgaggcact caagaaaatg ggactctaat actcttaagg
     1561 atttgtgatc atgagatccg ggaagaagga aagcggtttg attgacacca cagaataaga
     1621 aaaaggttgc cgtgatgggc ggcgcgttta atcccattca ctacggacat ctcagggccg
     1681 cagaagaggt gaggataaaa ctcggcttcg aaaaggtcct ctttattccc tcgggcaatc
     1741 cccccctcaa gatgaaagac cttgcagatg ccaaccaccg atacgagatg acgagactct
     1801 ccatagagac aaatcctttt tttgatatct ctgatattga atgcagggga agtcgaaaat
     1861 catacacagt ggaaacgctt acggcgttgg gggaaaggca ccccgagaat gagttcttcc
     1921 tcattttagg aaatgattcg ttccttgaca tccccgcgtg gtatcagccg gaaaggctca
     1981 cggagcttgc caattttgta gtagtttcga gacccggttt ccgtttttcc ggtctttcac
     2041 ctgaaatccc gatagcgcca gagactctat tcgcgcttga tgcccgtgct ctggaaatgc
     2101 atcgcctgcg tctggagggc gggagagaac ttctcctgct caatatcacc cctatggata
     2161 tatcggcaac agccgtgaga acgcttgtaa aagccgggaa gagcataaaa tatctattgc
     2221 cagagcaggt ggaatcctat ataattcttc ataagctgta tagagaaggg agtgatcacc
     2281 tttagaggga aaagataagg ccatcgctgc tgctacgtcg gcgctcgcaa aaaaggcaaa
     2341 ggatacgctc atccttgatc tcagaggatt agtcgtcttt acggattatt ttgtaatctg
     2401 ttccggagaa agtaccactc aggtaaaagc aatagcagag catatcgagg cagagtttat
     2461 gaaaaattcc ctcaagccgt tatcgattga gggatccagc tatgcccatt ggattctcat
     2521 agactatggt gatgtggttg tgcacgtatt ccagaatgag acaagggact attacgaatt
     2581 ggagaaactg tggctcgatg caccgaggat tcctttggag agggaaaaca cctctttaac
     2641 gggtgagagg tatcatttct gatgggtaag ctcgcagtgt tgctttttgt agtcattctc
     2701 gtcgcattgg gctatcttgc ggtactcaac aaagagccta tcacatttgc tctcgccccg
     2761 aaaacggtat acgagatgcc gaaaatcgcc cttatcctgc tctcatccgc agtgggcgct
     2821 gcagtgatgc tgctgatatt cttcattagg gacaccaagc ggttcattga gggcagacaa
     2881 taccaacgca agcagaagaa ggatatgatt gtccaggaac tttactccaa ggccttgaac
     2941 gccattcttg ccgacaacga agaggaggcg cgctctgccc tcgagagtgt cctcaaggaa
     3001 gaaccgcgac atatggatgc cctcttgaga ctcggtgata tcgcggcagg cgatgaagac
     3061 caccagaagg ccatgaacta ttaccgaaca gccggcgaga ttcaacctca cagtctcgaa
     3121 gtgctctttt cccttgaaag gatcatgggg aagaccggca ggaccaccga agcgctcatg
     3181 tacctcgata agattctcga actggacccc gataatcttg ccgccctcta cagaaagcgc
     3241 acgcttttag aaaggaagga aaaatgggat gacctcatct cactccagag ggcgatcatc
     3301 aagtgtgagc acaatgagaa agaccgcgag cgggagcaga taaacctcct ggggtacaag
     3361 tatgagcagg gtcgttacca cctcgaaaac ggtgaactgg aaaaggcaaa aaaggcgttt
     3421 aagaccatat tacgaatgga tatgaacttt gtgccggcat atctcggcct ggcagaggcg
     3481 atgctcaggg aagaggacac ggaagatgcc atagccttcc ttgaaaaggg ttttgcacag
     3541 acgtcctctc ccattatcct ggcacggata gaggaccttc tcataaatct gggggagccg
     3601 ggcaggctca ttatgcttta caagaacagc atattgaagg aaccgaagaa ccaggtcctc
     3661 cgattcttta tgggtaaact cttctacaga ctggaaatgc tggatgatgc ctttgacacc
     3721 ctcattgcgg tggatgggac aagcgcacct tatccggaac ttcatcagct cttgggcaac
     3781 atctacttga gacgtcagca atgtgagagg gcggtggaag aatttaagaa agtaatcgac
     3841 atcaaaaaac ccttcctcct cccttactgc tgcaacagct gcgggtatgc atctcttgat
     3901 tggtcgggca gatgtcccaa ttgcagggag tggaatacct atgagtttaa cctccatggt
     3961 atttgcagga cgcagtgacc gtaatgagtg atgaatacac accatagcga ggaaagcccc
     4021 tgtgatccct tgcccgtgat gccccacacg gcgcctcacc gggttgtttt ggatacaagc
     4081 ctcttcgtga atcctgatgc aaggacaagt cttggcaata ccccaaccga ggcgttggaa
     4141 acgtttcttt ttcttgcggc acagatccat atccttgaat tttatatgcc cccctccata
     4201 tttgaggaac tcttgcattt tgtggaaaag gataagatac ccggggatct cctagtcatt
     4261 ctccatcaaa aacctcccaa gaggcatgaa ctgaaatgtc ccgcggttct cctttatgaa
     4321 ttaatcgacg atatgcggga aagggtgaac aagggtctca gaattgcaga gaaggcggtg
     4381 aggggcgcgg aaaaaaataa ggcggatgag atgattcagg atctgagaag gaaatacagg
     4441 gaggcgctga gggaggggat aatcgacagt aaggaggatg tggatctcct cttgctcgca
     4501 atggaactgg atgccctcct tatcaccgct gaccagggtt tgatacgttg ggcagaaaaa
     4561 ctggggatac aatggctttt ccccgaaaag tttaaagact acctcatggg agcgataaag
     4621 agaacagagt taatctccgg gcagagagaa taattcgtca gcagttgtcg cctgcaccgc
     4681 actcatttcc tgtagcagag acagtgagaa ccctggaccg cgttcctcag ctcaaaccgt
     4741 tccttgatat tcctgataat ttcttgccgc gttctttccc atttgggagc aatgagaata
     4801 tcatccggag ccatggcaaa gagtctctgg aggacaattt cgggagaaag acgctgaatg
     4861 aactccacaa ggaaatccag atattcatca ta
//
LOCUS       DATKOU010000169         5434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2545532, whole genome shotgun sequence.
ACCESSION   DATKOU010000169 DATKOU010000000
VERSION     DATKOU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545532"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VMU21_07725"
     CDS             complement(<1..1075)
                     /locus_tag="VMU21_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HUO77453.1"
                     /translation="MKKNIYLKSTPLDEALGVWEIRLKREGLLRPLAPESVQVSDSLG
                     RVTAEALIAKISSPFYHAAAMDGYAVRFAETFGASETHPVKLRMGEQAFYVDTGDPMP
                     GGFNAVVMIEEVNKIGSGKSEVDGGRAATETADSAEYIEIIEPATPWQHVRTIGEDIV
                     ATELILPENHKIRPMDIGALLAGGHTDIMVRRRPLVAVIPTGDELIEPGTTLSRGSII
                     EYNSRVLGSLVHEWGGEPIRFSIVPDDISKLKEAVRKACGSADLVVVNAGSSAGSEDY
                     TARAIQELGEVVIHGVGIKPGKPVVLGIVRGKPVLGIPGYPVSAFITFHLFAGPLIHL
                     WQGIEKKSPERIRAKLSRQVASSL"
     gene            complement(1231..1680)
                     /locus_tag="VMU21_07730"
     CDS             complement(1231..1680)
                     /locus_tag="VMU21_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77454.1"
                     /translation="MTEWVFVSSPAAKDAHKNLYHFSETSYIITGEDLMESLIGFRGN
                     LSRLHIASTWTIMVGLAIWRARPHRGDMFLLLRNAGISAEELAPFRESEVGDIFPWLY
                     YGKRFDVLRRICQTAKASTERHLKEKDTRVHCHLVVEDIPQIIASSL"
     gene            complement(1677..2942)
                     /locus_tag="VMU21_07735"
     CDS             complement(1677..2942)
                     /locus_tag="VMU21_07735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HUO77455.1"
                     /translation="MPSQGVISPRIVRAEMPVYGGYVICKDGKVVFIRGAIPGELVEI
                     SIHDKKRDYSLAAARHIIEPSPYRRQPPCPVFGTCGGCQLQFMEYEKQVSVKEEILLD
                     SLRRIGDLDITLMPSMVGREFRYRHKAQLKVSPHGAIGFYKEGTREVVDIEECPLMVD
                     EINGFLRRARALDLKGVKELHVSSGDTMTLLVKGTPAEDTVQSLMETGISGIAFENGD
                     SVGKDYVTLDLNGLRYSVTPWSFFQSHWSLNREVVEEVIRQLTPMEKTRVLDLYAGAG
                     NFSLPLSRIAQEVVAVEENSYAVEDGRRNLVLNGIKNCIFVHGRVGKNLMSKKKDAVT
                     KLFEEPTYGCVILDPPRTGLPTDCIKKVMDGGSERVVYVSCNPATLARDVKKMREQYD
                     VESVRMVDFFPNTYHIEALTFLRRKASHA"
     gene            complement(2972..3322)
                     /locus_tag="VMU21_07740"
     CDS             complement(2972..3322)
                     /locus_tag="VMU21_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77456.1"
                     /translation="MKLLIVDDDQHIRRLYKEELEEEGYEVVVASTGSEALELFEKEN
                     PDLVTLDILMPDIDGIKLLRQMKEKKPRVPIIMSTAYDYRDDFAVWASEAYIVKSAEL
                     SDLKETIKKLLGKK"
     gene            complement(3319..>5434)
                     /locus_tag="VMU21_07745"
     CDS             complement(3319..>5434)
                     /locus_tag="VMU21_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO77457.1"
                     /translation="ECAVFILDITDRVLYQEEMIALYREVTQTKEYLESLIENSADAI
                     VTSDLNGVVTSWNRGAERIYGYTEEEAMGKYLPFVPEFLKETEKEYTEKVRNGEVMKD
                     IETFRQKKDSTLIEVSLTLSPIKDASGAVIGVSGISRDISGKKAVEKELIKRNQELSR
                     LFFISSAMRSTLDLNRLLRMILTAVTMGDGLGFNRAVLFLVDEERKVLKGAMGVGPAS
                     YEEAGRVWERLSKEKKTLAEVMQDIEAGIQEGDTFLDRLSTGIEIPLDADSVLALSVK
                     EKQLFNVPDARHEHLSDAVLIQQLGTEAYAVVPLISRDKVIGVLFVDNFFNKKPITEE
                     DMRFLTAFSNQMAAAIEGAKLFAQVSLAEAELENIFRSISDMVYFTDMDYTIKSVNKA
                     VADRIGTSEGKIIGKKCYEVFHGMNEPWQDCPHHKTVEHKKAYIEEVADPRTGETFLS
                     STSPLFDLSGKFLGTVHILRNISELKQIRERLATSERMAALGEVAAKVAHEIRNPLVS
                     IGGFAQRLEHKLDGNLKEYATIIAREVKRLEDILRDILGFVREVRLSMTPVNINTLLR
                     DVLSLVETEIMERGITLETAFAEVPDILLDMDRMREAFLNIVNNAIQAVGNRGRIKIL
                     TYPWNGYIVVEIQDTGKGISEKDLPFIFDPFYTTKPTGTGLGLAITRRIIEEHKGRIE
                     VKTQIEEGTTMKVFLSSGKEEG"
BASE COUNT         1255 a         1541 c         1139 g         1499 t
ORIGIN      
        1 ccagtgatga cgcgacctgt cttgacagtt tcgcccttat tctttcagga gatttcttct
       61 cgattccctg ccagagatgg attaggggac ctgcaaaaag atgaaatgtg ataaatgctg
      121 acaccggata tccgggaatc cccaatacag gtttccctcg cacaattccg aggactaccg
      181 gctttcccgg ttttatcccc acgccatgaa taaccacctc accgagctcc tgtatggctc
      241 ttgcggtgta atcctctgag cctgccgaag atcctgcatt cactaccacg agatcagcac
      301 taccgcaggc cttccttact gcctctttga gcttgcttat atcgtcaggg actatggaaa
      361 atcgtatagg ttccccaccc cattcatgca caagactgcc caaaaccctg gaattgtatt
      421 caataataga ccccctgctc aatgtggtgc caggttctat aagctcgtca cccgtgggta
      481 tgaccgctac taggggcctt cttctcacca ttatgtctgt atgaccccct gcaagcaatg
      541 cccctatgtc catgggccgt attttgtggt tttcggggag tataagttct gtggcgacaa
      601 tatcttcgcc aatggttctc acatgctgcc atggcgttgc aggctctata atctcgatat
      661 attccgctga atcggcagtt tcagttgctg cacgcccacc gtcaacctct gatttcccac
      721 tgcctatttt atttacctcc tctatcatga ccaccgcatt aaagcccccg ggcattgggt
      781 caccggtgtc cacgtagaaa gcctgttcac ccatcctgag cttcacggga tgggtctcgg
      841 aagcgccaaa ggtttcagca aatctcaccg catacccgtc cattgccgct gcatggtaaa
      901 agggcgatga gatctttgca atgagggcct cggcagtcac cctgcccagg gaatcgctga
      961 cctgaacgga ttcaggagca aggggcctaa ggagcccttc cctcttgagc cttatttccc
     1021 atacgcccaa cgcctcgtcc agaggcgtac ttttaagata tatgttcttt ttcatgcctt
     1081 tgtttctctc cccagatcaa ttcactttca caaatgcata ccatgggaat aaaggatata
     1141 tttacttcct tggttcaacc gtattcatac tactccgttg atatccctat gcatacgtca
     1201 cggaatccca cacggagatc gtcagcatct tcaaagactg ctcgcaataa tttgaggaat
     1261 gtcctctacc accagatggc agtgaacgcg tgtgtccttc tccttcaagt gcctctcagt
     1321 gctggccttg gctgtctgac atattcttct gagcacatca aaccttttcc cgtaatagag
     1381 ccatggaaat atatcaccta cttcagattc cctgaaaggg gcgagctctt cagcgcttat
     1441 ccctgcattt ctgagaagaa ggaacatgtc ccccctatgt ggcctcgccc tccatatggc
     1501 aagcccgacc ataatggtcc aggttgaggc tatatgcagt ctgctgagat ttcccctgaa
     1561 ccctatgagg gattccatga gatcctctcc tgtaatgata taggaggtct ctgagaaatg
     1621 atagaggttc ttatgagcgt ccttagccgc aggacttgag acaaagaccc attcagtcat
     1681 gcatgagacg cctttcttcg aagaaacgtc aaggcctcta tatgataggt attggggaaa
     1741 aagtccacca tgcgaaccga ctccacatcg tactgctctc tcattttttt tacatctctt
     1801 gcgagagtag caggattaca ggacacatac actactcgtt cgctgcctcc atccatcacc
     1861 ttctttatgc agtcggtagg aagcccggtt ctcgggggat caagaattac gcagccataa
     1921 gtgggttctt cgaaaagttt tgttactgcg tctttctttt tactcatgag atttttccca
     1981 actcttccgt gaacaaatat gcaattcttt ataccattga gtacgaggtt ccttctgcca
     2041 tcttccaccg cataggaatt ttcctccact gccaccactt cctgtgcaat gcgggaaagc
     2101 gggagtgaaa aatttcctgc acccgcatag agatccaaga cacgtgtctt ctccattgga
     2161 gtgagctgcc gtatcacctc ttcaaccact tctctgttga gggaccagtg gctctgaaaa
     2221 aaactccaag gggtcacaga atacctgagg ccattgaggt cgagtgtgac gtaatccttc
     2281 ccgacggaat caccattctc aaaagcgatt cccgatattc ccgtctccat caaactctgt
     2341 actgtatctt cagccggggt tcccttgacg agaagggtca ttgtatcccc tgatgacaca
     2401 tgtaactcct taactccctt gaggtccaag gcccgcgctc tccttaagaa accattaatc
     2461 tcgtccacca taagagggca ttcctcgata tccaccactt cccgtgttcc ctccttgtaa
     2521 aacccaattg ccccatgagg agaaaccttc aactgggcct tgtgcctgta tctgaattcc
     2581 ctaccgacca tggagggcat gagggtgata tccaagtcac caatccttct cagtgagtcg
     2641 agcagtatct cctccttcac cgacacctgc ttttcgtatt ccatgaactg taactgacag
     2701 ccaccgcagg ttccgaatac tggacagggt ggctgtcttc ggtaaggtga cggctctata
     2761 atgtgtctcg cggcggcgag agagtaatcc ctctttttat cgtgaatgga gatctccaca
     2821 agttctccgg ggatggcccc cctgatgaat acaacctttc catccttaca gattacgtat
     2881 cccccgtaca caggcatttc cgcccttacg atgcgcggtg aaattacacc ctgtgaaggc
     2941 actgctggtt tttcaatttc ctggggcagc gtcatttttt gccaagaagt ttttttatag
     3001 tctcttttaa atcacttagt tctgctgatt ttacgatata cgcctccgag gcccacaccg
     3061 cgaaatcgtc ccgatagtcg tacgcggtcg acatgatgat aggcactctg ggtttctttt
     3121 ccttcatctg ccgcaagagt tttatcccat caatatcggg catgaggata tcaagggtaa
     3181 cgagatcggg attctctttc tcgaacagtt ccagagcctc cgaaccggtc gacgctacca
     3241 caacctcgta tccttcctct tcaagctctt ccttatagag acgtctgatg tgctggtcat
     3301 catccacaat caaaagcttc atccctcctc ctttccactt gagagaaaga ctttcatggt
     3361 agtgccttcc tcgatctggg ttttcacttc tattctcccc ttgtgctctt ctatgatcct
     3421 gcgggtaatg gcaagaccaa ggcctgtgcc tgtgggtttt gtggtataga aaggatcgaa
     3481 aataaagggg agatcttttt ctgatatccc ctttcccgta tcctgtattt ccacaactat
     3541 atatccgttc cagggatagg tgagtatctt gatcctcccg cggtttccca cagcctgtat
     3601 cgcattgttc actatattca aaaacgcctc tctcattctg tccatatcca gaagaatatc
     3661 cgggacctcg gcaaatgcag tctcaagggt tatccctctc tccatgattt ccgtctctac
     3721 cagggagaga acgtccctga gaagggtatt gatattcacg ggagtcatag agagcctgac
     3781 ctccctcaca aacccgagga tatccctcag gatgtcctca agtctcttca cctcccgtgc
     3841 gatgatggtc gcatactctt taagattccc gtcaagctta tgctccaatc gctgtgcgaa
     3901 tccaccgatg gatacaaggg ggttccttat ctcatgggcg acctttgccg caacctctcc
     3961 cagggcggcc attctctccg aagttgccag gcgctccctg atctgcttga gttccgagat
     4021 gtttctcaga atatggacag tccccaaaaa cttccccgag aggtcgaaca atggggaagt
     4081 cgaggaaaga aacgtctctc cggtgcgcgg atctgccacc tcctcgatat atgccttttt
     4141 gtgttcgacc gttttatggt gcggacagtc ctgccatggc tcattcatac catggaacac
     4201 ttcataacat ttctttccta taatcttacc ctcactcgtt cctatcctat cggcaaccgc
     4261 tttgttcaca ctcttaatag tgtaatccat gtccgtaaaa tataccatgt ccgatatgga
     4321 cctgaagata ttctccagtt cagcctctgc cagggaaacc tgtgcaaaca gctttgcgcc
     4381 ctcaatggcc gcagccatct gattggaaaa ggcggtaagg aacctcatgt cttcttccgt
     4441 aataggtttt ttgttaaaaa agttatccac aaaaagcacc ccgatgacct tgtcccggga
     4501 gatgaggggg acaaccgcat acgcctctgt acccagctgc tggatgagca ccgcgtccga
     4561 aaggtgctca tgtctcgcgt cagggacgtt aaataattgc ttttccttta cagaaagggc
     4621 aaggacagaa tccgcatcca aggggatctc gatgcccgtg ctcaggcggt caagaaatgt
     4681 atcaccctcc tgaattcctg cctcgatgtc ctgcatcacc tctgcaaggg tcttcttttc
     4741 cttggagagc ctctcccata ccctgcccgc ctcttcatag ctcgcgggcc cgacacccat
     4801 ggcccctttt aataccttcc tttcttcatc caccaggaag agcactgcac ggttgaaacc
     4861 aagaccatcc cccatagtca ctgcagtcaa tatcatcctc aggagcctgt tgagatccag
     4921 cgtgctcctc atggcagagc ttatgaaaaa aagccttgaa agttcctggt tacgtttgat
     4981 taactccttt tccaccgcct ttttccctga aatatccctg gatatcccgc tcacccctat
     5041 aacagcccct gatgcgtcct taatgggaga aagggtgaga ctcacctcaa taagcgtgct
     5101 atcttttttt tggcggaagg tctcgatgtc cttcatcacc tcgccgttcc tgaccttttc
     5161 ggtatattcc ttctcggtct ctttcagaaa ttcaggtaca aaaggcaggt atttccccat
     5221 tgcctcttct tcagtatatc cgtatatcct ttcggcaccc cgattccagg aggtaaccac
     5281 accattaagg tcggaggtca caatagcatc cgcagaattc tctataagac tctcaaggta
     5341 ttcctttgtc tgtgttacct cccggtagag cgcaatcatt tcctcctggt agagaaccct
     5401 gtctgtgata tcgaggataa agaccgcgca ttcc
//
LOCUS       DATKOU010000170         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2157763, whole genome shotgun sequence.
ACCESSION   DATKOU010000170 DATKOU010000000
VERSION     DATKOU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157763"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2948
                     /locus_tag="VMU21_07750"
     CDS             <1..>2948
                     /locus_tag="VMU21_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HUO77458.1"
                     /translation="FSELALKSKPARIAGRCSVFAKSDMIHLQQIATPVEDIVAGLCF
                     AVARNFKGSIVRGRTIEEPVSFHGGVAANKGMVRAFREVFGFSQFLVPEDFALMGALG
                     AALKDLDEGTVNRFDLSLLRSLIESSQFSESGHRPLVSPGDDFFERHPEDIPQWEAIR
                     KKPEETIKLTTSGDPASTPKIRAYLGIDVGSISTNLAVIDDNGHLLAKRYLMTAGRPI
                     EAVNQGLREIGDELGDRVQIVGVGTTGSGRYMIADYVGADIVKNEITAQATAAVSINE
                     SVDTIFEIGGQDSKYISLKDGVIVDFEMNKACAAGTGSFLEEQAEKLDISVKNEFAGC
                     AFSSETPCRLGERCTVFMENSLMANLQRGAEKNDLLAGLAYSIVQNYINKVVTGKAVG
                     ENIFFQGGVAFNKSVVAAFEKYLGKKITVPPHHDVTGAIGMALIVMRHTQGIAASDSR
                     RTSFKGFALSERSHEISSFECKGCPNVCEINRVKIAEEEGYLFYGGRCERYDVKRQKE
                     PSALPDLFTFREEMLWKEHTKRLSKAGNGQTTAIGIPYLFFFHDYLPFWTTLLWELGF
                     HVEVSPKTNREIVNTGAESVLADTCFPVKVAHGHITHLIEKGVTTLLIPSFINMNDTD
                     EEFQRGYACPLTQSIPYAARVAHPEASILAPIVNLRAGKKSLINEMLRVLKPFKIRRA
                     KLLEAVKTAEKAQKEFSASIETKGAEVFSCLQERSIIIVGRPYNAFESGMNLNIPKKL
                     SDLNVLAIPMDFIPSAGLRISDTWPNMYWRSGQRILRAARFIRDHPHLFAIFIGNFSC
                     GPDSYLLKFFTEEMQGKPFLQIEIDEHSADAGAMTRCEAFLDSIGQRDHMTAHTAKDK
                     EEHRTAARFHRSHPFEKRTVFMPRMTDHAFAIAAAFERCGVPAEVLPESDRDSADIGR
                     RYVSGKECYPCTVTTGDMLKKVFSPDFEPERSAFFMPSGTGPCRFGQYNVFHRMVLSE
                     LGYHNIPILSP"
BASE COUNT          764 a          735 c          781 g          668 t
ORIGIN      
        1 ttcagtgaac tggcattgaa atccaagccg gcgcgcattg cgggcagatg cagcgtcttt
       61 gcaaagtcag acatgatcca cctccagcag atcgctactc ctgtggagga catcgttgcg
      121 ggtctctgct ttgcggtggc aaggaacttt aaaggttcta tcgtaagagg acggacgata
      181 gaggaaccgg tttctttcca tgggggagtt gcggcaaata agggaatggt aagggcattc
      241 agggaggtct tcggcttctc gcagttcctc gttcccgaag acttcgcgct gatgggcgca
      301 ctgggtgctg cgctcaaaga cctggatgag ggaacagtaa accgctttga tctctccctg
      361 ctcaggtcac tcatagaatc atctcaattt tccgaatccg gccacagacc gctcgtctct
      421 cccggcgatg acttcttcga gagacatccc gaagacattc cccagtggga agctatacgt
      481 aaaaagccag aagaaaccat aaaactcaca acatccggtg accctgcgtc aactcccaaa
      541 atcagggcgt acctcgggat cgatgtggga tccataagta ccaatctcgc agtgatcgat
      601 gacaacgggc atctccttgc aaagagatat ctcatgactg cgggaaggcc cattgaggca
      661 gtgaaccagg gcctcaggga gataggagat gaactgggtg acagggttca gatagtgggc
      721 gtaggaacaa ccgggtccgg gagatatatg atcgctgact atgtaggtgc cgacattgta
      781 aaaaatgaga taactgccca ggcaaccgct gccgtctcca taaatgagag tgtggatacc
      841 atctttgaga tcggaggcca ggactcaaaa tatatatccc tgaaggacgg tgttattgtg
      901 gattttgaga tgaacaaggc ctgtgccgca ggtaccggct cttttctcga ggaacaggcg
      961 gaaaagttgg atatctcggt gaagaatgag tttgccggat gtgcgttttc ctcagaaact
     1021 ccctgcagac tgggagagcg gtgtacagtt tttatggaga attccttgat ggctaacctc
     1081 cagaggggcg cagagaagaa cgacctcctt gcaggccttg cgtactccat agtccagaac
     1141 tacatcaata aagttgttac cggaaaagcc gtgggtgaga acatattctt tcagggaggg
     1201 gttgccttca acaaatcagt ggtggcagca tttgaaaaat accttggcaa gaagataacg
     1261 gtgccgccgc accatgatgt gaccggagca ataggcatgg cactcatcgt catgcgacat
     1321 acccagggta tagcagcctc tgactcccgg cgcacttctt tcaaggggtt tgccctttcg
     1381 gagcggtccc atgaaatctc gtcttttgaa tgcaagggat gtcccaatgt atgcgagatc
     1441 aacagggtga agatcgcaga agaagaaggc tatcttttct atggaggcag gtgtgaaaga
     1501 tatgatgtaa aaaggcagaa agaaccttct gcccttccag acctctttac cttcagggag
     1561 gagatgctgt ggaaagaaca taccaaaagg ctctcgaagg ccggcaatgg acaaaccacc
     1621 gcgataggga ttccttacct cttcttcttt catgactatc tccccttctg gacaaccctc
     1681 ctctgggaac ttggattcca tgtggaggtc tcaccaaaga cgaacaggga gatcgtgaac
     1741 accggggccg aaagcgtcct cgctgacaca tgcttccctg tcaaggttgc ccacggccat
     1801 ataacacatc tcatcgaaaa aggggtcact accctgctta tacccagttt tatcaatatg
     1861 aatgacaccg atgaggaatt tcagagaggg tacgcatgcc ctctcacgca gtcgatcccc
     1921 tatgcagccc gggtcgcgca tcctgaggca agtatcctgg cccccattgt gaatttgagg
     1981 gcaggaaaga agtccttgat aaatgagatg ctgagggtcc tcaagccctt taaaataagg
     2041 agggcgaagc tcttggaggc cgtgaagact gcggagaagg ctcagaaaga gttctcggcc
     2101 tcaatagaga caaagggggc tgaggtcttt tcttgccttc aggagagaag catcattatt
     2161 gtcggaagac cctacaatgc ctttgaaagc ggcatgaatc ttaacattcc caaaaaactc
     2221 tctgacctta atgttcttgc aattcccatg gactttattc cttctgcagg cctcaggata
     2281 agtgatacgt ggccgaatat gtactggagg tccggccaga ggatactaag ggctgcacgc
     2341 ttcataaggg accacccgca cctcttcgct atttttatag gaaacttctc gtgcggtccc
     2401 gattcctacc tcctgaaatt ctttactgaa gagatgcagg ggaaaccctt cctccagatc
     2461 gagatagacg aacacagtgc tgacgcggga gccatgacaa gatgcgaggc attccttgac
     2521 agcatagggc agcgggatca catgaccgcc catacggcga aggacaagga ggagcaccgc
     2581 accgccgcgc gcttccatcg cagccatcct tttgaaaaaa ggactgtctt catgccaaga
     2641 atgacagacc acgcgtttgc cattgccgcc gcctttgaac gatgcggcgt gcctgcagag
     2701 gttcttccgg aatcggacag ggattctgct gacataggga ggcggtacgt ctcgggcaaa
     2761 gagtgttacc cctgtactgt caccacagga gacatgctga aaaaggtctt ctccccggat
     2821 ttcgagccag agcggtctgc ctttttcatg ccctcaggaa caggaccctg cagattcggt
     2881 cagtataacg tgtttcaccg gatggtgctt agcgaacttg gctaccataa tatacctatt
     2941 ctttcacc
//
LOCUS       DATKOU010000171        16927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3700338, whole genome shotgun sequence.
ACCESSION   DATKOU010000171 DATKOU010000000
VERSION     DATKOU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 16927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16927
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3700338"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1439
                     /gene="lpxB"
                     /locus_tag="VMU21_07755"
     CDS             258..1439
                     /gene="lpxB"
                     /locus_tag="VMU21_07755"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HUO77459.1"
                     /translation="MIVAGESSGELYGSLLAHTLRAMWPEVRLVGMGGERMRDAGVEL
                     FSGISSSLGLTETLSSIKKLRESFTKASRILREIAPEIVVLIDYPDFNFRIARIAKRH
                     GVKVLYYVSPQVWAWRKGRVKTMGEIADRVAVILPFEEEIYKKAGIPCEFVGHPVMEE
                     IDALEKTNAQEVEHLRPGSGIHTPTVSLLPGSRPHELKTLFPVFTGFVERFTHKFPGV
                     RFLVPLAQNVEVDQFKESISALEGKGVVFMRGDALKSLVSSDMAVIASGTATLQAALL
                     GTPLVVVYRVSPLTYFIGKSVLDVTHISLVNIIAGKEVVPELIQHEATAKNIVAQSRK
                     ILYDDKYRESMISSFRKIRELFSGKQPSRRVAEMIGEMAGWEYGKAGAVSPNNSQRRN
                     A"
     gene            1436..3205
                     /locus_tag="VMU21_07760"
     CDS             1436..3205
                     /locus_tag="VMU21_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO77460.1"
                     /translation="MKDLKKILVLVKPYWGRLIVAGICSLVVSGLNGSLAWLVKPAMD
                     KVFVQKEPAFLVLLSLAVMTAYLGKGVFSFFQSYLMRSAGAKIVRDIRNKLFHHLLYL
                     PMGFLHRDSTGAMVSRIINDAGFVQGLLAYTVKDIFVEGATIMVLVCVAMYRRWDITL
                     ISIVVLPVAFYFVSRLGKRLKKVSLHTQEKIEEITEILTETFSGNKIIKSFCREEDEM
                     RRFRGKNHDFYRELMRSTRIIEGTSLMMEFVGGLGIAFVLWYGGSLVIRGTITAGDFF
                     SFLVAIFMIYTPAKRLVAANNSLQQASAPLDRIDRLLEEETEPDGTIALEGFHNEIAF
                     NHVSFRYQGTDDDALDDISLTVKKGEIIALVGRSGAGKTTFVDLIPRFYTPGGGKIFI
                     DSVDISGVTLKSLRSLIGIVTQDVILFNDTVRANIAYGDARASEEDIVNAAKAAYAHE
                     FILQLPHGYDTVVGERGIRLSGGQKQRLSIARAILKNPPILILDEATSSLDTASEIMV
                     QRALENLMNDRTAFVIAHRLSTVRRATRIIVMDKGKIVESGTHEALLAFDGIYQRLYS
                     LQFDDAMADKLLSESPLITERHS"
     gene            3207..4523
                     /locus_tag="VMU21_07765"
     CDS             3207..4523
                     /locus_tag="VMU21_07765"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HUO77461.1"
                     /translation="MFFAYSLLYTVALLALLPYEYFRRPKSLRMRWLRERCGLTAPFS
                     TTGHSSAIWVHAVSVGEVISAIPLIREIKGRYPSLRIVISTVTDTGQKLARERISDLA
                     YPVYLPFDLALVLKKVIKSLKPRLFIVMETELWPNTFRVLRKEGIPILVVNGRISDRS
                     FKGYKKIRFFIKEVVKWVDMFCMQEEVYKKRIQALGADEDRIMVTGNFKFDITPLDRI
                     PEWSGSLRGPVIVAGSTHEGEEDLVVSVFMRLRNEHSELNLIIAPRHPERFHKVEDML
                     RAQGLPYIKRSEMSPRATDRGQERGEDQRLSGTIIILDTIGELAPAYGMAEVAIMGGS
                     FASHGGHNPLEPAFWGKPVVCGPHMENFPFIQDFYTERAAIKTEEEGLYGVLNELLRS
                     PEKRTTMGKKARLLYDEKAGAVHRAMEILERYLKAELPVAKEESFP"
     gene            complement(4467..7448)
                     /locus_tag="VMU21_07770"
     CDS             complement(4467..7448)
                     /locus_tag="VMU21_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HUO77462.1"
                     /translation="MVFLKKMKMMSLVLLAFVTSMVFLSPLPCFSDELPRIATSLSFD
                     MEKRVMEGKSSIAFPDHGEIRVHTGPLKIKSLRYNHRSLSPVIREGILTLEGEKGGVL
                     DMEYECASNGHGPCSIDTKGIFLTGNWYPSVEGPAYHRLRALVPRYFTAISEAEEIKK
                     EKMPGGNLFTFVFPHPVEDITFLAGPFSIKRETFRGIELYAYFLSEDKRLAKNYLDHT
                     KKYLGLCERKMGKYPFKRFSVVENIFSTGYSVPTFTLLGQDVVRLPFIVNTSLGREIL
                     HQWFGNLVYVDYEKGNWAEGLTTYLSDQLYKEREERGWQYRKQTLIDYDTYVNPENEF
                     PLRDFRSGTDSASRAIGCGKGSMVFHMLKNMVGEERFFKSIRKFINENRFRRASWDDL
                     RTAFEVESEEDLDQFFGQWLNEKGYPSLELSNVEAGPKGRHYAVSFDIVQKGKPYLLD
                     FTVAVKTEAGDTDRVLRLYDEKQDFDILTEGKPEGLVIDEYYDTFRKLTPAETPPVIG
                     RLLNHDKGLIVLPSQGSEEIYSRIVKYFEDRGYGTKKPQEVESKDLKQSPLVVLGYDN
                     PVIKRLFGKMDEPSQGLVLSMRKNPFDPSMIIGVINASSGNEVDAAWEKIPHYGNFSL
                     LAFKEGKNTETRIKESQRGWDVPLGEPVVGIEVSKTKKLPDIINSVSDKRIVYVGERH
                     TSYEHHLTQLETIKGFFRKNPKIAIGMEMFQRPFQPVLDDYIAGKIDEKEFLKSSEYF
                     SRWVFDYHLYRDILTFAREERIPVVALNIKREIVDKVSKNGIDSLTEEEKKELPDSMD
                     MSDEEYRERLREVFEKHESAGSRDFGNFYQSQIIWDEIMAQSIDEYLRKNPDRKMVVI
                     AGSGHLAFKSGIPKRVFRRNSLDYALLLNDESVETGIADFVLFPQPVKMVSAPKLMAL
                     LSEEKGKVTIKGFPDMSVSEKAGMEKGDIIISMDNEPIKGVDDIKIFLLYKKPGDTID
                     VRVLRKRFLFGDRELSFKVTL"
     gene            complement(7513..7944)
                     /locus_tag="VMU21_07775"
     CDS             complement(7513..7944)
                     /locus_tag="VMU21_07775"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HUO77463.1"
                     /translation="MHRVMFLCTANSCRSQMAEGLAREFGKGVLEVYSAGLMAAGVHP
                     RAIAVMKEIGIDISRQRSEEIDEKLLRTMDVVITLCGHAEKYCPRTPPGITRIHWPVE
                     DPVGTLGTEERIMYEFRRARDEIKEKILNFVKEIKDSRKIL"
     gene            8256..8858
                     /locus_tag="VMU21_07780"
     CDS             8256..8858
                     /locus_tag="VMU21_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77464.1"
                     /translation="MAVCIVMAVISLSILWCGAALASKESILSDKNEFGGKTIEVIIE
                     KDGAPPEIQKILASYDEADKMRKTEITYTEAFGNEKGVAKAIEYLKPDGKVAKREYYY
                     TDAFGNKKGVARATEYYYANEKVAKAEFIYTETFANEKGVTKGTEYYDENGKVAKAVF
                     TYSDAFAKEEGVNKRIEYYDSDGKVVKREFYRDNILMRGE"
     gene            complement(8920..9477)
                     /locus_tag="VMU21_07785"
     CDS             complement(8920..9477)
                     /locus_tag="VMU21_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77465.1"
                     /translation="MKQPHILYRKATPSDFEGILLLQHQNLLTTLQGEDLSQGFLSIE
                     FTREQLHKVNSELGIFVAVQGKAVVGYLMAETAEFAIGSPLISNMLKRLKDVVFDDVT
                     LSCCFFVYGPVCIDRQSRGQGILEGLFVIMKETLRADYDVGIAFVSQQNLRSIQAHTA
                     KLGMRVIDEFKFKGERYGTLAFRLK"
     gene            9665..10312
                     /locus_tag="VMU21_07790"
     CDS             9665..10312
                     /locus_tag="VMU21_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HUO77466.1"
                     /translation="MKVMAFNGSPRKEWNTATLLEKTLEGAASQGAKTELIHLYDLNF
                     KGCISCFACKTIGGKSYGKCAVKDDLTNVFNRIEQANAIVLGSPIYWGDVSGEMKSFI
                     ERLMFQYTTYTDPPGSLFSNKIKTGFIYTMNVTEEIMKEWGYAVNFNRYEAALKRIFG
                     ESEYMCCYDTYQFYDYSKVLATRFDPVKKKQRHEEVFPLDCQKAFEMGVRLAGGK"
     gene            complement(10328..10585)
                     /locus_tag="VMU21_07795"
     CDS             complement(10328..10585)
                     /locus_tag="VMU21_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77467.1"
                     /translation="MVLVLCSGEEPWNGLLIPTDHQFHDDILKDFKGMVQHPVLKKWL
                     YLPEASEDFETVAMDLLEAIRLGDQRIGIEPKPRGSRKRKS"
     gene            complement(10894..11307)
                     /locus_tag="VMU21_07800"
     CDS             complement(10894..11307)
                     /locus_tag="VMU21_07800"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUO77468.1"
                     /translation="MNLEMVNSFIKEVGWGFLATSDGNKVGIRPIGSCVWMGNELWAA
                     TTEGTEKIMQLRKIPYAEYCFSDREGKHVRIAGPCTISTNNDDKLKLYEAVPILKNYI
                     KEPTEPKYVVIRMKPERIRFMPQAGKGYEDIVVQK"
     gene            complement(11400..11801)
                     /locus_tag="VMU21_07805"
     CDS             complement(11400..11801)
                     /locus_tag="VMU21_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77469.1"
                     /translation="MTLKAYLESREGKGILSSANSGGMVTSAIYSKPHVFDDGTIAFV
                     MRKKLAYENLSTNPYASYMFIEEGYGYKGIRLLLKKIKEETDSKLIDKMKRQYLTPEE
                     DKAKGPKFLMHFRVDTILPLIGEGDTGIEIK"
     gene            complement(11810..12283)
                     /locus_tag="VMU21_07810"
     CDS             complement(11810..12283)
                     /locus_tag="VMU21_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HUO77470.1"
                     /translation="MEKEPSSRLETVSHVDLNQYMGVWYEIALYPNSFQKGCVGSRAT
                     YTLLDDGKVSVLNECYDGSLNGKLRSAKGKAWVVDKETNAKLKVSFFWFFAGDYWIID
                     LGKDYEYAVIGHPKRKYLWILSRTKEMDGAVYDGILSRLQDKQYDTSKLIRTLQK"
     gene            complement(12737..13201)
                     /locus_tag="VMU21_07815"
     CDS             complement(12737..13201)
                     /locus_tag="VMU21_07815"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HUO77471.1"
                     /translation="MNNKPKPLSHEELKKLRKPIKNVNAEHRETLTRLERLAVWITEK
                     VGSMGFFLIIFAWTIVWLGWNTITPKELRFDPFPAFVLWLFISNVLQIFLMPLIMIGQ
                     NLQGRHSESRAESDFEVNVKAEREIEAILLHLENQNELILKILSKIEADRVK"
     gene            complement(13615..13691)
                     /locus_tag="VMU21_07820"
     tRNA            complement(13615..13691)
                     /locus_tag="VMU21_07820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13655..13657),aa:Arg,seq:tct)
     gene            complement(13817..13894)
                     /locus_tag="VMU21_07825"
     tRNA            complement(13817..13894)
                     /locus_tag="VMU21_07825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13857..13859),aa:Pro,seq:tgg)
     gene            14016..14885
                     /locus_tag="VMU21_07830"
     CDS             14016..14885
                     /locus_tag="VMU21_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HUO77472.1"
                     /translation="MMNILMSGATGFIGGHLSRAFRDKDWKVIPLGRDDLKLPDEDFL
                     ERMEGADVVINMAGASIAERWTEEHKQTMYSSRVDTTKKIVNALEKMSRKPGVFISTS
                     AVGIYDTEGEHTEEDTHYANDFLGKLALDWEHAAHKAGELGVRTVIFRFGVVLGLDGG
                     ALQKMLIPFRMGLGGIIGNGKQPFSWVHIEDLIRAYTTVIENDAYHGVYNLTSPHPTT
                     NAGLTKALGHALHKPAFMHVPAFAFRLQLGEGAKVLLEGQRVLPKRLLESGFTFRFTE
                     IETALEDLMGKER"
     gene            15110..16366
                     /locus_tag="VMU21_07835"
     CDS             15110..16366
                     /locus_tag="VMU21_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77473.1"
                     /translation="MKDDNKTKKELIDELDGMRRRIDELETICRDMTDQKRHEAQLSQ
                     EQKMEVIGQMAGDVAHDFNNILTAIIGYASILQAEANNQQRVYVEQILHSAEKASHLT
                     QGLLSLSRKQLLKARPTNLNESVKGIEKLLSKIIGEDIALRTRFANEPLTVLADEVQL
                     EQVLINLATNAKDAMPRGGTLTIETMLSELNQRYCETHGYGIPGNYALISVTDTGVGM
                     DQKKSERIFEPFFTTKEGGKGTGLGLSVVYGIIKQHNGYINVHSQPGRGTTFNIYLPL
                     ITAEEKKKRPAGSSGFPQGTGTILLAEDDTDVRNLSRWALEEFGYEVIEASDGLEAIR
                     KFTENRDKIQLLLLDVVMPHKNGAEVFDEIRKLKPDIKVLFMSGYPADLLRKEDVLEE
                     GSHFIAKPISPKNLLREVNRVLETEG"
     gene            16457..16753
                     /locus_tag="VMU21_07840"
     CDS             16457..16753
                     /locus_tag="VMU21_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77474.1"
                     /translation="MQLLILLVWLSAICIIFFALYRALLLKRKIPGGLVKQSWKLLYY
                     LIGLLGVGYLTMPLFPGLPDTSRDLIVGIISLAGAIFIVKVINLFYRIIKEVGL"
BASE COUNT         4540 a         3967 c         4001 g         4419 t
ORIGIN      
        1 tgcaatgagc tttctgggtt acagggaggg tgcctttccc gtttctgaga aggcatccag
       61 ggaagtgtta tcattgccca tctatgctga acttggagag ccggatatag agaaaatagc
      121 aggagttata aaaagggttg cccaatagtg gttcatagcc tgttaggaaa aatcccttta
      181 aaggaccaga aagtaattca tactctccgc atgcataagc cggcgtatca atagccctgg
      241 aaactatgaa gacggtcatg atagtggcgg gcgagagttc tggtgaactc tacggttctc
      301 tcctcgctca taccttacgt gccatgtggc ctgaggtgag gctggtgggc atggggggcg
      361 aaaggatgag agacgcggga gttgagctct tctccggcat atccagttcc ctaggcctca
      421 cagaaaccct ctcatccatt aaaaagctcc gcgaatcctt tacaaaggcc tcgaggatat
      481 tacgggaaat cgctccagag atcgtggtac tcattgatta tcctgatttt aactttagga
      541 ttgccaggat tgcgaagagg catggggtga aggtgctcta ttatgtgagt ccgcaggtat
      601 gggcgtggag aaaaggaagg gtaaagacca tgggagagat tgcggacagg gtggcggtga
      661 tcctcccttt tgaagaggag atttataaga aggccggcat tccctgtgaa tttgtgggcc
      721 accctgtgat ggaagagata gatgcgttgg aaaaaacaaa cgcacaggaa gtggaacacc
      781 tcaggccggg atccggaatc catactccaa ccgtttccct tctccccggc agcaggcccc
      841 atgaactgaa gacactcttt cctgtcttta ccggctttgt ggagagattt acccacaaat
      901 ttcccggagt gcggtttctc gtacctcttg cgcagaatgt ggaggttgac caattcaagg
      961 aaagcatctc agcgcttgag ggaaagggtg tcgtgttcat gagaggtgat gccctcaaat
     1021 cccttgtctc atcagacatg gcggtcatcg cctctggtac tgcaaccctt caggccgcgc
     1081 tcctgggaac acccctggtg gtggtgtaca gggtctcccc cctgacttac ttcatcggga
     1141 agagcgtact ggacgtcaca cacatttccc tcgtgaatat tatcgcggga aaagaggttg
     1201 tcccggaact tatccagcat gaggcaaccg caaaaaatat cgtggcacaa tccaggaaga
     1261 ttttgtatga tgataagtac agagagtcca tgatctcttc attcaggaag ataagggagc
     1321 tcttctcagg aaagcagcca tcccgaaggg tggcagaaat gataggggag atggcagggt
     1381 gggagtacgg gaaagccgga gccgtctccc ctaataattc gcagaggcgg aacgcgtgaa
     1441 agacctaaag aagatcctcg tgctggtgaa accctactgg ggacggctca ttgtcgcagg
     1501 catttgcagc cttgtggttt cgggactgaa cgggtcactg gcgtggctcg tcaagcctgc
     1561 aatggacaag gtgtttgtgc aaaaggaacc cgcattcctc gtcctgctga gtctggctgt
     1621 catgaccgcc tatctcggca aaggggtctt ttcttttttt cagtcctatc tcatgaggtc
     1681 ggcgggcgcg aagatcgtaa gggacataag gaacaaatta ttccaccatt tgctttacct
     1741 ccccatgggt ttcctccata gagactccac cggcgccatg gtctccagga taattaacga
     1801 cgcagggttt gtccaggggc tcctcgccta caccgtaaag gacatttttg tggagggcgc
     1861 gaccataatg gtccttgtct gtgtagccat gtacaggcga tgggacatca ccctcatatc
     1921 tatcgtggtg ctgcccgtcg ccttctactt tgtttcaagg ctcggaaaga ggctcaagaa
     1981 ggtcagtctg catacccagg agaaaatcga ggaaattacc gagatcctta ccgagacctt
     2041 ttctggcaac aagatcatta agtccttctg cagagaagag gatgaaatga gaagattcag
     2101 gggcaagaac cacgactttt accgggaact catgcggtcc acaaggatta ttgagggaac
     2161 ttccctgatg atggagttcg taggaggttt gggtatcgca tttgtcctct ggtacggagg
     2221 gagtcttgtg atcagaggga ccattaccgc gggagatttc ttctcctttc tcgtggccat
     2281 cttcatgatc tatacccctg cgaagcgcct tgtggctgca aataattccc tccagcaggc
     2341 aagtgcgccc cttgacagga ttgacaggct tctggaagaa gaaacagaac cggatggcac
     2401 catagcgctg gaagggttcc acaatgaaat tgcgttcaat catgtctcct tcaggtatca
     2461 gggaaccgat gacgatgccc tggatgacat ctcgctcact gtgaagaaag gagagatcat
     2521 cgcccttgtg ggcaggagcg gtgccggcaa gaccaccttt gtggacctca taccgagatt
     2581 ctacacccct ggagggggaa agatattcat agacagcgtt gacatttccg gagtcacgct
     2641 gaaatccctc cgctccctta tcggcattgt gacccaggat gtcatcctct ttaacgatac
     2701 cgtgagggca aatattgctt acggagatgc cagggcatcg gaggaggata ttgtaaacgc
     2761 agcaaaagct gcatacgccc atgagtttat cctccagctt ccccatggct atgacacggt
     2821 ggtaggggaa aggggaatca ggttatcggg aggacagaaa cagaggcttt ccatagcaag
     2881 ggcaatattg aaaaaccctc ccatacttat tctggatgag gccacctcat ctctggacac
     2941 cgcttcggag attatggtac agagagccct cgaaaatctt atgaatgaca ggaccgcctt
     3001 tgtgattgct cacaggcttt ccaccgtgag aagggcgacc aggatcatcg tgatggacaa
     3061 aggcaagatc gttgagtcag gtacacatga ggcactcctc gcgtttgacg gcatttacca
     3121 gaggctgtac agcctgcagt tcgacgatgc aatggcggac aaactgttga gtgaatctcc
     3181 cctgatcacc gaacgacact cctgagatgt ttttcgcgta tagcctcctt tacactgtcg
     3241 ctttgctcgc tctgctgccc tatgaatatt tcaggagacc aaagagcctc aggatgagat
     3301 ggctgagaga aagatgcgga ctgaccgctc ctttttcgac tacaggtcat tcttcagcca
     3361 tatgggtcca tgcggtttcg gtaggagagg tcatttccgc tattccgttg atacgggaaa
     3421 ttaaggggag atacccttcc cttcgcattg tcatttccac ggtgaccgac acgggacaga
     3481 aactcgcccg cgaaaggata tcagaccttg cgtatccggt atacctgccc tttgatctcg
     3541 cacttgtgct caaaaaagtg ataaagagtc tgaaaccccg gctcttcatt gttatggaaa
     3601 cggagttatg gcccaatacc ttccgggtat tgaggaaaga gggaattccc atcctcgtcg
     3661 ttaacgggag aatatctgac cgttccttta aaggatacaa gaaaataagg ttctttatta
     3721 aggaagtggt caagtgggtg gatatgttct gcatgcagga ggaggtttat aagaaaagga
     3781 ttcaggcact gggcgctgac gaagacagga tcatggtaac agggaatttt aagtttgaca
     3841 taacaccatt agatagaatc ccggaatggt caggatccct gagaggtcct gtaatcgtcg
     3901 cgggaagcac ccatgaagga gaggaagatt tggtggtgtc tgtttttatg aggctgagga
     3961 atgagcattc cgaactcaac ctcatcattg cgccccgcca ccctgaaagg tttcacaaag
     4021 tagaagacat gctgagagca caggggctcc cttacatcaa acgctctgaa atgagtccac
     4081 gggcaacaga caggggacag gagagagggg aagaccaacg gctttcggga acgatcatca
     4141 ttctcgacac cattggcgaa ctcgctccgg cctacggtat ggctgaagtg gccatcatgg
     4201 gggggagctt tgcaagccac gggggacaca atccccttga acctgcgttt tggggaaaac
     4261 cggtggtatg cggcccccat atggaaaatt tcccctttat ccaggatttt tatacggaga
     4321 gagcagccat taaaaccgag gaggaaggtc tgtacggcgt tctcaatgag ctgctcaggt
     4381 cccctgaaaa gagaaccacc atgggaaaaa aggcgaggct tctctatgac gagaaggcgg
     4441 gtgcggtgca cagggccatg gaaatattag agcgttacct taaagctgag ctccctgtcg
     4501 ccaaagagga atcttttcct taacacgcgc acgtcaatgg tatcccctgg ctttttataa
     4561 aggaggaata ttttgatatc gtccacacct tttatcggtt cattgtccat ggaaataata
     4621 atgtctccct tctccatccc tgccttttcc gacacgctca tatcggggaa tcccttgatt
     4681 gttacttttc ctttctcttc ggacaagagc gccatgagct tgggtgctga aaccattttt
     4741 accggttgcg ggaagagcac gaaatccgca attcctgttt ccacagattc gtcattgaga
     4801 agtaatgcat aatccagact attccgcctg aataccctct tgggaatgcc ggacttaaag
     4861 gcaagatggc cgctcccggc aatcaccacc atcttccggt cagggttctt tctcaggtat
     4921 tcatcaatgg actgggccat tatctcgtcc cagataatct gggattggta aaaattgccg
     4981 aaatcccttg acccggcgct ttcatgcttt tcgaacacct cccttaacct ctcccgatac
     5041 tcctcgtccg acatgtccat actgtcaggc aactcctttt tttcttcttc ggtaagggag
     5101 tcgatcccgt ttttcgatac cttgtccaca atctccctct taatgttcag ggcaaccacg
     5161 ggtatcctct cctccctcgc aaatgtcagg atatccctgt acaggtggta atcaaatacc
     5221 catcggctaa aatactcaga ggacttcagg aactcctttt catctatttt acccgcgata
     5281 taatcatcga gcaccggctg gaagggccgc tggaacatct ccattccaat ggcaatctta
     5341 ggattcttcc taaaaaatcc tttgatcgtt tcgagctggg tgaggtggtg ttcgtagctt
     5401 gtgtgacgtt cgcccacata cacgatcctt ttgtcagaaa cgctgttgat gatgtcaggc
     5461 aatttctttg tcttcgaaac ctcaatccct accactggtt cccctagagg cacgtcccac
     5521 cctcgttggc tttccttgat ccttgtttcg gtgttcttcc cctccttaaa ggctaagaga
     5581 ctaaaatttc cataatgggg tatcttttcc catgcggcat caacttcatt gcctgaggat
     5641 gcattgatca cgccaatgat cattgaagga tcgaacgggt tcttccgcat actgaggaca
     5701 agcccctgtg acggttcatc catcttcccg aacagcctct ttatgacagg gttgtcatac
     5761 cccagaacaa ccagagggga ctgctttaaa tccttgctct ccacctcctg gggtttcttc
     5821 gtcccatatc ccctgtcctc aaagtacttg acaatccttg aatagatctc ttcactgcct
     5881 tgagacggaa ggacaatcag tcccttgtca tggttcagga gtcttcctat tacaggaggg
     5941 gtttcagcag gagtaagctt tctgaaggta tcgtagtatt cgtcaatgac cagcccttcc
     6001 ggctttccct cagtgaggat atcgaagtcc tgcttctcgt catagagccg aaggacgcgg
     6061 tccgtgtcac ccgcttctgt ctttaccgcg acagtaaaat cgagcaggta cggttttccc
     6121 ttctgcacaa tgtcaaatga gacagcatag tggcggccct ttggtcccgc ctctacattg
     6181 gagagttcca gggagggata ccctttttca ttcagccact gcccaaagaa ttgatcgaga
     6241 tcttcctctg attccacttc aaaggcggtt ctcaaatcat cccatgatgc cctgcgaaag
     6301 cggttctcat tgatgaattt cctgatggac ttgaagaacc tctcctctcc caccatgttc
     6361 ttgagcatgt ggaacaccat cgatcctttg ccacagccta tggccctgga ggcagagtcg
     6421 gtcccggacc tgaaatcccg caggggaaac tcattctcag ggttgacgta ggtgtcataa
     6481 tcgatgagcg tctgtttcct gtactgccat cccctttcct ctcgttcttt ataaagctga
     6541 tcggaaagat acgtagtcag cccctcagcc cagttccctt tttcgtaatc cacgtacaca
     6601 aggtttccga accattgatg aagtatctcg cggccaagag atgtattcac aataaagggc
     6661 agcctcacca catcctggcc aagaagggtg aaggtcggca cggaatatcc ggtggaaaag
     6721 atattctcca caacagaaaa ccttttgaaa gggtatttcc ccatctttct ttcgcagagt
     6781 cccaggtatt tttttgtgtg gtcaagatag ttcttcgcaa gccgtttgtc ctcagagagg
     6841 aaatacgcgt acaattcaat accccgaaaa gtttctcttt tgatactgaa cgggccggca
     6901 agaaaggtta tgtcctctac cgggtgagga aacacgaagg tgaaaagatt tcctcccggc
     6961 attttttcct tttttatctc ttcagcctct gaaatggccg tgaaatacct ggggaccagt
     7021 gccctgagcc tgtgatacgc cggtccttcc acagacggat accagttgcc tgtgagaaat
     7081 atgccctttg tgtctatgct gcatggccca tgcccattcg acgcacattc gtattccata
     7141 tcaaggacgc cgcccttttc cccttccagc gttaagatac cctccctgat cacgggcgag
     7201 agagaacggt ggttatacct cagggacttt attttgagag gaccggtatg gacacgtatt
     7261 tctccatggt cgggaaaggc gattgaggac ttcccctcca tcaccctctt ttccatatca
     7321 aaggacaggg aagtggcgat gcgcgggagt tcatccgaga aacaaggaag gggagaaaga
     7381 aataccatag aggtaacaaa cgcaagcagc accagggaca tcattttcat ttttttaaga
     7441 aagaccatcg tattctctca cacacccagg aaggagaacc ctcatcgaca caaaccattc
     7501 ccgtttcgca tatcagagaa tcttccggga atccttgatc tcttttacga aatttagtat
     7561 cttctccttg atctcgtccc ttgcccttct gaattcatac atgattcgtt cctctgtgcc
     7621 aagggtaccc accgggtctt ccacaggcca gtggatgcgt gttatcccag ggggtgtcct
     7681 cgggcagtat ttctcggcat ggccacacag cgtgatgacg acatccattg ttctcaacag
     7741 cttttcatct atctcctctg acctttgacg tgagatgtcg atgccaatct ctttcatcac
     7801 cgcaatggcc cttgggtgca cccctgcagc catgagaccc gcactgtaca cttctagtac
     7861 tcccttgccg aactcccgtg caaggccttc agccatctgg ctgcggcatg aattggcggt
     7921 gcagagaaac atcactctat gcattctttt tctctcattc agtatcttaa aagaaaaacc
     7981 ccttggaaca gcaaggagaa caggaacagt ggttgagcca gcgttttttt gctctcaacg
     8041 cccttcataa aattatagca catctgctct ttcatctctt cccttgcgga gcagaatcct
     8101 ctttactcga tgtgagattt tagtatagta aaatgagggc cagccttgac tcaactgttt
     8161 acaatctttt cctaaaagtg tatagttctt tcacatagag aattggattt gcagtctctt
     8221 tcagacaaac tgtgtgggag gatcaaggga tgcgcatggc ggtatgtata gtgatggcag
     8281 taatcagtct ttctatccta tggtgcgggg cagcattagc ttcgaaggag agcatccttt
     8341 ctgataagaa tgaatttgga ggaaaaacaa ttgaagtgat catcgaaaag gacggggcac
     8401 ctccagaaat acaaaaaatt ctcgccagct atgacgaagc ggacaagatg cgcaagacag
     8461 agatcaccta taccgaggcc tttggcaatg aaaagggagt ggcaaaggcc atagaatacc
     8521 ttaagcctga cggaaaggtg gcaaagcggg aatattacta taccgacgcg tttggcaaca
     8581 aaaagggagt ggccagggcc acggaatact attatgccaa tgaaaaggtc gcaaaagcgg
     8641 aattcatcta taccgagacc tttgcaaacg aaaagggggt gacgaaaggc accgaatact
     8701 atgatgagaa cggaaaggtg gccaaggcgg tattcactta cagtgatgcc tttgcaaaag
     8761 aagagggagt gaataagaga atagaatatt atgattccga cggaaaagtg gtgaagcggg
     8821 aattttaccg cgataacatc cttatgaggg gagaataatc cccgtgtact gccaggtgtc
     8881 caagaaacta tagggtttct tgcaagatgg tatagactct tatttcaacc taaaagcaag
     8941 ggtaccgtat ctctcaccct taaacttaaa ctcatcaatg accctcatac ccaattttgc
     9001 ggtgtgtgcc tgaatggaac gaaggttctg ctgtgagaca aaggctatcc caacatcata
     9061 atcagccctc agggtctctt tcatgatcac aaacaatcct tcaaggattc cttgtcccct
     9121 tgattgcctg tcaatgcaga caggtccgta gacaaaaaaa caacatgaca aggttacatc
     9181 atcaaagaca acatccttca gccttttgag catattgctt ataaggggcg agccaattgc
     9241 aaattctgct gtttccgcca tgaggtatcc aacgactgcc ttgccctgaa cggcaacgaa
     9301 aataccaagt tcactgttta ccttgtgaag ttgttctctt gtgaactcga tggacaggaa
     9361 tccttgggac aggtcttctc cctggagtgt cgtcagaaga ttttgatgct gaagcaggag
     9421 aatgccctca aagtcagaag gggttgcttt acggtatagg atatggggtt gtttcattga
     9481 ggtttttatt ataatagaat atatggcaaa gaaacctcat aagaagatca ccagcacccc
     9541 gtctctccac gaaataaaag aaaaaagtag aataaaaagc atcctcaaaa agttaataca
     9601 catcaagaag ggacattgaa gaagccagag ataaaaactg aaacaacaaa aaaggagaca
     9661 taacatgaaa gttatggcat ttaatggaag tccaaggaaa gaatggaaca cggcaacatt
     9721 gcttgaaaag actcttgaag gagctgcctc acaaggagca aaaacagaac tcattcatct
     9781 gtatgacctt aactttaaag gatgcataag ctgttttgcc tgtaagacca taggtggtaa
     9841 aagttatggc aaatgtgccg tcaaggatga cctgactaat gtcttcaata ggattgaaca
     9901 ggctaatgcc attgtcttgg gttcccctat ttattggggt gatgtctctg gcgagatgaa
     9961 atcatttata gaacgtctaa tgtttcagta caccacctat actgatcctc caggttcact
    10021 tttctccaat aagattaaga cagggttcat ctataccatg aacgttaccg aggaaatcat
    10081 gaaagaatgg gggtatgctg ttaatttcaa ccgttatgaa gcggctctca aaagaatctt
    10141 cggggagtct gaatatatgt gttgttatga cacctatcag ttttatgatt attccaaggt
    10201 gctggctacc cgttttgatc ctgtgaagaa aaaacagagg catgaagaag tattccctct
    10261 ggattgccaa aaggcatttg aaatgggtgt gaggttggct ggcgggaagt aacaggaagg
    10321 ggtcgggtca ggatttcctt tttcttgatc cccgtggctt cggttcaatc ccaatcctct
    10381 gatcccccag ccttatagcc tcaagcaaat ccatagctac ggtttcaaag tcctctgagg
    10441 cttctggcag atatagccat ttcttcagga cagggtgctg aaccataccc ttgaagtcct
    10501 tcaggatatc gtcatggaac tggtggtcag tcgggatcag taacccattc cagggttctt
    10561 cacctgaaca taaaaccaaa accatcaacc cgtggagata gatagggctc ctaatacaat
    10621 tgcgcttccc ttcttggatt gattagaagc aagggacgca ctataagctg ccgctccaca
    10681 ggttatccat cgtaacaaaa tatagtatcc ataaaataat ggtgcaagtg ctaatagcaa
    10741 cattgctgaa acaatacttc ttaatattat tgatgggggg attcttgaga gtttcatttg
    10801 cttatcactg cgtataacat aagtgtcaag cgccccaaca tcctcagaca atggggaagg
    10861 ctaggttaat cagtgaaaaa ggtatatggg gcattatttt tgcactacaa tatcttcata
    10921 tccctttcca gcttgaggca taaaacggat gcgttctggt ttcatgcgaa taacaacata
    10981 tttgggttca gttggctctt taatatagtt ttttagaatt ggaacagctt catacagttt
    11041 caatttatca tcattattgg tactaatggt gcaaggacct gcaattctaa cgtgctttcc
    11101 ttccctgtca ctgaaacagt attctgcata ggggattttt ctaagttgca tgattttctc
    11161 tgtaccctca gtagttgcag cccacagttc attgcccatc catacacagc ttccaatagg
    11221 tcgaatccct accttattgc cgtcacttgt agcaaggaat ccccatccga cttcttttat
    11281 aaaactatta accatttcta aattcatcgt agcactcctt tcattgcgta attcttatta
    11341 atattatatc tccaaatcaa cgcagaaacc accacgacaa agaagtagcc gtacattttt
    11401 tactttatct caattcctgt atctccctcc ccaatgagcg ggagtatcgt gtccaccctg
    11461 aagtgcataa ggaatttcgg tcccttcgct ttatcttctt caggtgtaag gtactgtctc
    11521 ttcatcttat caatcaactt gctatcggtt tcttctttta tctttttcaa caataatctt
    11581 attcccttat aaccataacc ttcttcaata aacatatagg aagcatatgg attggtcgat
    11641 aggttctcgt aggcaagttt ttttctcata acaaaagcaa ttgtaccgtc atcgaaaacg
    11701 tggggcttcg agtatatcgc tgatgtaacc ataccgcctg agttggcaga cgagaggata
    11761 ccctttcctt ctctgctctc taagtatgct tttagtgtca tgtctcctcc tatttctgca
    11821 atgttctaat cagttttgag gtgtcgtatt gtttgtcctg taagcgggag agaatcccat
    11881 cgtacaccgc tccatccatt tccttcgtac ggctcaaaat ccagaggtat tttctttttg
    11941 gatgtccaat aactgcatat tcgtaatcct tgccaagatc aataatccag tagtctcctg
    12001 caaagaacca aaaaaatgac accttgagct tggcgttagt ttctttgtca acaacccatg
    12061 ctttcccttt tgcagaccgc aacttcccgt tcaatgaccc gtcatagcac tcattcaaga
    12121 cagaaacctt accgtcatcc aacagtgtat aggttgccct gctccccaca cagcccttct
    12181 gaaaactgtt cgggtatagg gcaatctcat accacactcc catatattgg ttgagatcaa
    12241 catgagaaac tgtttccaaa cgtgatgatg gttctttttc catatatttg catcctacca
    12301 caacaagcaa agttgaagcg atgattccaa taaaaaggaa tgatatgtct ttgttcctca
    12361 tgtggatcct ttgcacttga tgacatcaca agcacaaccc ttttgccgta agatttctac
    12421 ctgtcttggt atcgggtcta caaatgcagt ctgagtccga ctccccttgg caagcgcctt
    12481 atccagggga ctcttatgat tgtagcctct tgccagcatc tcctgaacaa tttcctcatg
    12541 aaccctgaac agtgccttca gcttcccttt ccatctcttt gtctcaggat gatcggcata
    12601 cccttgtttc ccctgtgtca gtatgttcca tatcgcatga agttcattat gctcaccaag
    12661 gagatgatta cggcaaagct tatcaggagg gatatcccat atcctcatgt ttttgtattt
    12721 cggtgttggt taaaaattac ttcactctgt cagcttctat tttactgagt atctttagga
    12781 taagttcatt ttgattttca agatgaagca atattgcttc tatttccctc tcggctttta
    12841 cattaacctc aaaatcagac tcggctcttg attctgaatg tcgtccctga agattctgtc
    12901 ctatcataat taagggcatc aggaaaatct gtaacacatt ggatataaaa agccacagga
    12961 cgaaggcagg gaaagggtca aaccgtaatt ccttgggtgt gattgtgttc catccaagcc
    13021 aaacaattgt ccaggcgaag attatcagaa agaaacccat tgagccaacc ttttctgtaa
    13081 tccatacggc taatctctcc agtcgggtca gggtttctct atgctcagca ttgacattct
    13141 ttatcggttt acgcagtttc ttcagttctt catgagacag tggttttggt ttgttattca
    13201 tcgctacctc catgtagtta atgcggtttc ccttttatgg ggtttccttt tttctttctg
    13261 ctattatatg catctttctt accaaccttt caaccctgtt ccccatcctt ttcacccccc
    13321 agaacaaaac atctcacagg ggagagggga atgtcctgcc cagtgtctag aaaaggtctc
    13381 ggagggttgt ggggttggtt tttgagccta ttggctggat atggctttag cttgcactcg
    13441 atccagaggg gtagggaacg atgttttcct tggaaggttg tgggtgcgga ggcaaaaaat
    13501 tgggtgcatt ttgggtgcaa aaaagcacta ttttgccctg tttttcccca ttttccgaaa
    13561 ccaataaaaa aggcttctaa gggtcataac cccttgaaac ccttgtgttt gtaatggcgc
    13621 gcctggagga actcgaatcc ccgacccact gcttagaagg cagttgctct atccaactga
    13681 gctacaggcg caacaaaaag ttatcagtga cgaggaacag tgatcaatga caatcaaaat
    13741 cacatcatct aagtccacga acctgttcac tcacaactaa agactttcac tgcaaattgt
    13801 acggagcaaa gggtgctggt cggggcgaga ggattcgaac ctccgacccc ctgcgcccaa
    13861 ggcaggtgcg ctagccaggc tgcgctacgc cccgaactta ttatcttaat gaattcaaag
    13921 accaaaaatc aaatgaccaa atcctgcgag cgcacatcat aaagattcaa agttcactta
    13981 tataaagagt tctgataaga tgaacggaag aggtaatgat gaatattctc atgagcggcg
    14041 ccacaggctt catcgggggc catctttcga gggcatttcg agacaaggac tggaaggtta
    14101 tccccctcgg gcgtgatgat ctgaagcttc ctgatgaaga ctttctggaa agaatggaag
    14161 gcgctgatgt ggttatcaac atggcggggg cttccattgc agagagatgg acagaggaac
    14221 acaaacagac tatgtattcc agcagggttg atactacaaa aaaaatcgtg aacgcattgg
    14281 agaaaatgag caggaagccg ggcgtgttca tttccacctc tgccgtcggt atctacgata
    14341 cggaggggga gcacactgaa gaagatacac attatgccaa tgatttcttg gggaagctgg
    14401 cccttgactg ggagcacgca gcacataagg caggagaact gggagtgaga acggtaatct
    14461 tccgctttgg ggtagtgctc gggctcgatg gcggagcgct ccagaagatg cttataccct
    14521 tcagaatggg attgggcggt attatcggga atgggaagca gccattctca tgggtgcata
    14581 tcgaggacct catcagggca tacaccaccg tcatagagaa cgatgcctac cacggggtat
    14641 ataatctcac atcgcctcat ccgaccacga atgcaggttt gacaaaggcg ctgggacatg
    14701 ccctccataa gcctgctttc atgcatgttc ccgccttcgc attcaggctc caattgggtg
    14761 aaggcgccaa ggttctgctt gagggccaga gggtcctccc caaacggctt ctggagagcg
    14821 gctttacctt ccggttcaca gagatcgaaa cagcattgga agacctgatg ggtaaagaaa
    14881 gataattacc cttagtagta ttccaggtca tatcgtctta tttccggtca cctgttgagc
    14941 cacctgctat tcttgcccac atattcctga atacgagaga caatcactaa ccacttacag
    15001 aataagaata tttctgattt ttcagatgtt ttgtcatcag gtactgacaa cgggcatggc
    15061 gtatgctatt atagcgcaca ataagctatt ggtagaggag cctatttgga tgaaagacga
    15121 caataagaca aaaaaggaac tcattgacga attggatgga atgagacgga gaattgatga
    15181 actcgaaaca atctgccgtg atatgacaga tcagaagaga cacgaggctc aactcagcca
    15241 agaacagaag atggaggtta tcgggcagat ggcaggggac gttgcccatg attttaataa
    15301 tatccttacc gcgataatag gctatgcgag catcctgcag gcagaagcga acaatcagca
    15361 gagagtttat gtggaacaaa tactgcactc agcagaaaag gcttctcacc tcacacaggg
    15421 tcttcttagc ctcagcagaa aacagctact caaggcaaga cctactaacc ttaacgagag
    15481 tgtgaaaggg atagaaaaac tactgtcgaa aattatcggc gaagatatag cactcaggac
    15541 tcgctttgca aatgaacctc tcacggtatt ggcagatgag gtgcagctag agcaggtatt
    15601 gattaacctt gctacgaacg cgaaagatgc catgccacgc ggaggaacgc tgaccattga
    15661 gacaatgctg tcggaattga atcaaaggta ctgcgagaca catgggtatg gaataccggg
    15721 gaactatgcc cttatttctg tcactgatac gggggtaggc atggatcaaa agaaaagtga
    15781 gaggatattc gagcccttct ttaccacaaa agaaggaggg aaagggaccg ggctcggact
    15841 ctcggtagtg tacgggatta tcaagcagca taatggttat atcaatgtgc attctcaacc
    15901 gggaagggga actacgttta atatatattt gccgctcatt accgcggagg agaaaaagaa
    15961 aagaccagcc gggagttctg gctttcctca gggtacggga acaatacttt tggcagagga
    16021 cgacacggac gtaaggaacc tctcgagatg ggcacttgag gaattcgggt atgaggtcat
    16081 tgaagcttcg gatgggctgg aagcaatacg taagtttact gagaataggg ataaaatcca
    16141 gcttctcctg cttgatgtcg tgatgcctca caagaatggg gcagaggtat ttgatgagat
    16201 aagaaaatta aaacctgata taaaggtgct ttttatgagt ggctatccgg cggatctgct
    16261 tcgcaaggag gacgttcttg aggaaggatc acattttatc gcaaaaccca tttcgccaaa
    16321 gaatcttctg agagaggtga atagggtttt agagacagaa ggctaactgt gtcatacatg
    16381 acgggagaag caataatcaa aagggtctct aaggaactcc ccaaaaaaga atcatcggga
    16441 tgaagagagg gaaacgatgc aactgctcat tttattagtc tggctgtcag caatctgcat
    16501 aatcttcttt gcattgtaca gagccttgct cctgaaaagg aagattcccg gagggctcgt
    16561 caagcaatcc tggaagctcc tttactactt gataggcctc ttgggggtag gatacctcac
    16621 catgccatta ttccccgggc tgcccgatac gtcgagagac ctcatcgtgg ggattatttc
    16681 cctggcaggg gcaatcttta tcgtaaaggt aatcaatctc ttttacagga taataaaaga
    16741 ggtgggattg tgacatggca accagagacg atctaaaaaa cattatccgg gaacaccttg
    16801 aaaagacatc cagctctatt ttcctggaca agtcgttggc tatcctgaac gaatcagcag
    16861 acagcaagga gagcctcatg gctgctgctg acaggatcag taagaggatt gcattgttca
    16921 tcgatga
//
LOCUS       DATKOU010000172         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4473971, whole genome shotgun sequence.
ACCESSION   DATKOU010000172 DATKOU010000000
VERSION     DATKOU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4473971"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..425)
                     /locus_tag="VMU21_07845"
     CDS             complement(237..425)
                     /locus_tag="VMU21_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HUO77475.1"
                     /translation="MCDLNAYIYQEGKEELYLENVNSAKSEEGKVYLRNLFGEQKVFE
                     GIIQEISLSGHRILLTKG"
     gene            715..1869
                     /gene="trpB"
                     /locus_tag="VMU21_07850"
     CDS             715..1869
                     /gene="trpB"
                     /locus_tag="VMU21_07850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HUO77476.1"
                     /translation="MYGGRFVPETLMPALVELEVTYREARADREFQERLTQLQKTYIG
                     RPTPLYFARRLTESLGGARVYLKREDLAHTGAHKINNAIGQALLAKKMGKKRLIAETG
                     AGQHGVATATGAALVGLECEIYMGSEDMQRQALNVFRMKLLGATVSEVPVGSRTLKDA
                     INEALRDWTTNVRTTHYVMGTVFGPHPFPMMVRDFQSVIGKETRKQILDAEGRVPDYL
                     VACVGGGSNAMGLFYAFLNDDVRMVGVEAGGKGIDSGEHAARFAGGSLGVFQGTKSYL
                     LQDEDGNVLGTHSVSAGLDYASIGPEHPYLRDTGRVQYTYATDDEALSAFEHLSRMEG
                     ITPALESAHAVAETLKLAPRLPGDSIIVVNLSGRGDKDVQQVARLKGIEL"
     gene            1874..>2270
                     /gene="trpA"
                     /locus_tag="VMU21_07855"
     CDS             1874..>2270
                     /gene="trpA"
                     /locus_tag="VMU21_07855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HUO77477.1"
                     /translation="MNRVEKVFKELKRKSRKAFIPYIMAGDPSPERTMDTVLMLERCG
                     ADIVELGVPFTDPLADGPTIQRAAERALKAGVTLRRVMALVKELRNHSAIPLLLMTYY
                     NPVFKYGEEGFVKDAVRAGIDGVIIPDLPP"
BASE COUNT          564 a          521 c          646 g          539 t
ORIGIN      
        1 caagttccta ttccttttag aaacttgcaa acccttttat gctctttgta aaatcataca
       61 ctgaaagaaa caggaattca acatcttttt ttgctctccg aatttcgatt gccaaatccc
      121 ccataatgct cctcacgata agcatatggg tacaatgttg aaaaagatat ttggcctttg
      181 gtcctatata ctggggcact ggggacgttc cggcagcacg tgaaggccat accggactac
      241 ccctttgtga ggagaatccg gtgaccggag agggaaatct cctggatgat cccctcaaat
      301 actttttgtt cgccgaagag gttcctgagg tataccttgc cctcttccga tttcgcgctg
      361 tttacgtttt cgaggtagag ttcctctttg ccctcctggt aaatatacgc attgagatca
      421 cacatagcac ctccgggaaa tacgtgattc ctgcaacaca tgcgagaaaa gtgtcctttt
      481 ccgttaccct gcagtataaa gaatgggcag aatgatgtca aacgaccaga gaaattatct
      541 gcccccatat ggaccgcgct caattccata gggttaaatt gatttggttt ggtcactgtt
      601 ccccggtatc ctgaacctgt catgcggtgc tcttatgcta taatagctcg tcattgtata
      661 gaattccgga ggggaatcat taagaaactt cctgacaaaa gagggtattt cggcatatac
      721 ggcggccggt ttgtgcctga aacgctcatg cccgcactgg tggaacttga agtgacctac
      781 cgtgaggcaa gggccgatcg tgaatttcag gaaaggctta cgcaactcca gaagacgtat
      841 atcggaagac cgaccccatt gtattttgcg aggagactca ccgagtccct tggaggagcg
      901 agggtgtatc ttaagcgtga agaccttgcg cacaccggcg cacataagat caataatgcc
      961 attggccagg ccctgctcgc gaaaaagatg ggcaagaaga ggctgattgc agaaacgggt
     1021 gcaggacagc acggggtggc caccgccaca ggggcggccc ttgtgggtct tgaatgcgag
     1081 atctacatgg gttcggaaga tatgcagagg caggcattga atgtcttcag gatgaagctc
     1141 ctgggcgcaa cggtgagcga ggtgcctgtg ggatcaagaa cactgaagga tgcaattaat
     1201 gaggcattga gggattggac gaccaatgtg aggaccacac actatgtaat ggggacggtc
     1261 ttcggccccc atccctttcc catgatggtg agggacttcc agtctgtgat aggtaaggag
     1321 acaagaaaac agatactgga tgcagaggga agggtgccgg actatctcgt ggcgtgtgta
     1381 ggcggaggca gcaatgccat gggattgttt tatgcgtttc tcaatgacga tgtgaggatg
     1441 gtcggcgttg aggcgggtgg aaaggggatc gattccgggg agcatgctgc ccgctttgca
     1501 ggagggtctc tgggcgtctt tcagggtacg aaaagctatc tcctgcagga tgaagatggc
     1561 aatgtcctcg gtacgcactc tgtctcggcc ggtcttgatt atgcatctat cggccccgag
     1621 catccttatc tcagggatac ggggcgtgtc cagtacacgt atgccactga tgatgaggca
     1681 ttgtcggcat ttgaacactt aagcaggatg gagggaataa cccctgccct ggagtctgcc
     1741 catgcggtcg cagagacgct caaacttgca ccaagactcc cgggggattc cattatcgtg
     1801 gtgaatcttt caggacgcgg ggacaaggac gtgcagcagg tggcaagact caaggggatt
     1861 gaactgtaac cagatgaaca gggttgagaa ggtatttaag gaactcaaaa ggaaaagcag
     1921 gaaggcgttc atcccttata ttatggcagg agacccttcc cctgaaagga ccatggatac
     1981 ggtcctcatg ctcgagagat gcggtgccga tattgtggag ttgggggttc cctttaccga
     2041 tccccttgcg gacggaccca cgatccagag agcagcggag agggccctga aagccggtgt
     2101 caccctgcga agggtgatgg cgttggtgaa agagttgaga aatcattcgg cgattcctct
     2161 tctgcttatg acgtattata accctgtatt taagtatgga gaagagggat tcgtaaagga
     2221 tgcggtgcgt gcaggcattg atggcgtgat catacccgac ctccccccgg
//
LOCUS       DATKOU010000173         8911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4858561, whole genome shotgun sequence.
ACCESSION   DATKOU010000173 DATKOU010000000
VERSION     DATKOU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4858561"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VMU21_07860"
     CDS             complement(<1..470)
                     /locus_tag="VMU21_07860"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HUO77478.1"
                     /translation="MEAKKVKESSVAIAQVMVPQDVNPSGNVHGGVIMRLIDTTAGVV
                     ASRHARSNVVTVSIDRLDFHHPVFVGDLLVLKGSLNMVGRTSMEIGVRVEAENLMTGQ
                     VRHTASAYLTFVALDEEGKPREVPPLILETEDEVRRNREAKVRRAQRVNERRQEA"
     gene            complement(527..1021)
                     /locus_tag="VMU21_07865"
     CDS             complement(527..1021)
                     /locus_tag="VMU21_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HUO77479.1"
                     /translation="MKLFFPKTLLTVLGIGLLLTVLASYVQAEADKNAKRIFTKHFQE
                     TLFDISKKAEFSIEILLDDKEYKIGKNVIGVVVHNANDKDVEKAAISIDFRNSDTGRP
                     STQTPVVKEKGEGLYIVSDLDLKKEGHWKLTIKVKKDGIEDSVQFLLPDALKTLHPAG
                     KYSP"
     gene            1107..1274
                     /locus_tag="VMU21_07870"
     CDS             1107..1274
                     /locus_tag="VMU21_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77480.1"
                     /translation="MTKYGTMRKRMAYYSWLNMRSLRNGAKELRPSEEHNQKQRLNST
                     TEILFIGKERI"
     gene            1271..2086
                     /locus_tag="VMU21_07875"
     CDS             1271..2086
                     /locus_tag="VMU21_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77481.1"
                     /translation="MSKKVFVGALIFVIVVGGIYFYTLTVVFPSPKNCSVCHFITPFY
                     KQWQTSTHNKVPCLKCHEYSPTKALVGQFMFLSGSYNPRPLTNVPDKNCLQSGCHDKR
                     MIDSKVTFTKRGITFDHKTHFTEMKRGITLHCRSCHSDIVQGSHIKVSMNVCYLCHFK
                     GVPHDQALTGCPSCHSSPTQPIVVMGKTFSHVEALKAGYKCNQCHIEVTKGDGVTPKD
                     KCYFCHVDRTEKYSDVKLIHKKHVTQKQIDCLWCHTKIEHGKIAMAEKIPLRR"
     gene            complement(2375..3391)
                     /gene="aroF"
                     /locus_tag="VMU21_07880"
     CDS             complement(2375..3391)
                     /gene="aroF"
                     /locus_tag="VMU21_07880"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HUO77482.1"
                     /translation="MIIVLKKGCKKKTVESIIKKVKDLGFRAHAIVGVERTVIGAVGD
                     DRDKHLLYALEAIPCVEKLVPILQPYKLASKEIKKEPTIVKVDSALFGGGHFGVVAGP
                     CSVESEKQIIETAIKVKKAGARMLRGGAFKPRTSPYAFQGLEEEGLKLLSKARQESGI
                     PFVTEVINPEDVELVGRYADVLQIGARNVQNFSLLRRVGKFGKPIVLKRGMSTTIQEF
                     LMSAEYILSEGCKEVILCERGIRTFENATRNTLDISAIPVIHERSHLPIIVDPSHATG
                     VSNYVSPLCFAAMACGADGIMVEVHPDPQKALCDGPQSLRVEDFAKLMEGLRKLAPFS
                     GKKL"
     gene            3628..4848
                     /locus_tag="VMU21_07885"
     CDS             3628..4848
                     /locus_tag="VMU21_07885"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HUO77483.1"
                     /translation="MLIVQKYGGTSVANVERIKAVAGRVVKATQEGNQVVVVVSAMAG
                     ETDKLINLAHKISQNPSDREMDLLLSSGERVTSALTAIAIESLGHKAMAFTGRQMGII
                     TDTVHTKARIEKITGERAKKVLDEGYVVVVAGFQGVTEHKDEVTTLGRGGSDLSAVAI
                     AQALNADLCEIYTDVDGVYTTDPNIVPEARKLDKISYEEMLELASLGAKVLQTRSVEF
                     AMNYGVPVVVRSSFNNNPGTLVTKEDKDMEKVAVSGVAYDKNQAKITVTGVPDKPGIA
                     AKLFKGIADANMIVDMIVQNVSSDGKAADISFTVPKTESKKALKMTEAIVRELGAKGV
                     TLRDDIAKISIVGVGMRTHSGVAAKMFDTLAGHGINIVMISTSEIKVSCVIDAKYTEL
                     AVRVLHDAFELEKA"
     gene            4893..6470
                     /gene="cimA"
                     /locus_tag="VMU21_07890"
     CDS             4893..6470
                     /gene="cimA"
                     /locus_tag="VMU21_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HUO77484.1"
                     /translation="MGKAARKIELFDTTLRDGAQTEDISFSVEDKLRIAERLDELGIH
                     YIEGGWPGSNPKDIEFFKKVKKLKLRNSRVVAFGSTYRPKHRVEDDANIKALLNAKTD
                     ILTIVGKTWDFHVREALRITLEENIEIIHTTILYLKKYVDKVFFDAEHFFDGCRDNPR
                     FSMKCVLAAQDAGADCIVLCDTNGGTLPNGVREVTEKVSGALSAPIGIHAHNDAECAV
                     ANSIVAVEAGASQIQGTMNGLGERCGNANICSIIPNLQIKLGLRTVTDEQLMKLREVS
                     RFVNEIANLRHFKRQPFVGDSAFAHKGGIHVSAIVKRPETYEHIRPESVGNSQRVLIS
                     DLAGKSNILRKAAEFHITLDPDAPQVQDIVTKLKDLENQGFQFEGAEASFELLMKKSL
                     GLHKTFFDLIGFRVIVEKRKEKEEALSEATIMVKVGGHIEHTAATGQGPVNALDNALR
                     KALEKFYPQLKEVRLHDYKVRVLAAGKGTSARVRVLVESGDEEIRWGTVGVSENIIEA
                     SWQALVDSIEYKLLRGE"
     gene            6529..7542
                     /gene="ilvC"
                     /locus_tag="VMU21_07895"
     CDS             6529..7542
                     /gene="ilvC"
                     /locus_tag="VMU21_07895"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HUO77485.1"
                     /translation="MKVYYDKDAKMSVLKGKKIVVVGYGSQGHAHANNLKESGLDVTV
                     AETKGSNWEKAEKAGFKVLTAAEAAKKADVIMMLVPDEYAADIYKTEIAPHIKKGAYL
                     GFAHGFNIHFGQIAPPQDVNVFMVAPKGPGHLVRAEYTKGSGVPCLIAVFQNPSKNAK
                     DVALAYASGIGGGRAGIIETSFREETETDLFGEQVVLCGGLTALIQAGYETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYEGGISTMRYSISNTAQYGDLTRGPRVITDETKKEMKKI
                     LTEIQDGAFAREWLLECKVNKPVFNALTRKGEEHPIEQVGAKLRAMMPWLKKGKLVDK
                     SKA"
     gene            7751..8386
                     /locus_tag="VMU21_07900"
     CDS             7751..8386
                     /locus_tag="VMU21_07900"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HUO77486.1"
                     /translation="MIKLAPEGNPFILGFLGLTILVDLFAPWGALLPFVLTLFMVYFF
                     RDPERRIPEGNNVLVSPADGKVILIQKVSEDKFLKADAVEVSIFMSPLDVHVNRAPCE
                     GVVEDVVHTSGRFLSAFRHEASLQNENIAMLLTTRYGRILVRQVAGFLARRAVCRVKT
                     GDSLRKGERYGIIKFSSRLDIYLPKEVQMRVKLGDRVKAGETVIGEMPSGH"
     gene            complement(8492..>8911)
                     /locus_tag="VMU21_07905"
     CDS             complement(8492..>8911)
                     /locus_tag="VMU21_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77487.1"
                     /translation="QNWEKGIDDQLTKLLKMDNPPPGLHTLLYRYLLPLEQEMNQLSL
                     LFIRRVLAKLHVNTTSGVYYLLCFAVVVIAVGKVLPSTPAIDLAVLNSAVAVVTYAVL
                     ELLFVLSFTLQEVREEYTEITDTVAEEEIQNDRTNYN"
BASE COUNT         2338 a         2006 c         2349 g         2218 t
ORIGIN      
        1 gcttcctgtc ttctttcatt aacccgttgc gctcttctta cctttgcctc acggttgcgc
       61 cgaacttcat cctcagtttc caggataagg ggtgggactt ccctcggttt cccctcttcg
      121 tccagggcaa caaaggtcag gtatgcggaa gcggtatgcc tcacctgccc tgtcatgagg
      181 ttttcagcct caacccttac cccgatttcc attgaagtcc tccccaccat atttaagctt
      241 ccctttaaca ctagcaggtc tcccacaaac acagggtgat ggaaatccaa tctatcgatg
      301 gaaacggtca ccacattact gcgcgcatgt ctgctggcta ccacccctgc ggtggtatcg
      361 atgagtctca tgatgacacc accgtgaaca tttcctgaag gattcacatc ctggggtacc
      421 attacctggg caatggcaac gctgctttcc tttacctttt tggcttccat agtaaagtgc
      481 cgacagggtt cattacgcgt tgttcatgca ttgtgagttg taaggcctaa gggctgtatt
      541 tcccggctgg gtggagggtc ttgagagcat ccgggagaag aaattgtacg ctatcctcaa
      601 tgccatcctt cttgaccttt atcgtgagct tccagtggcc ttcttttttc aggtcaaggt
      661 ctgacacgat gtaaaggcct tctcccttct cttttaccac aggggtctga gttgatgggc
      721 ggccggtatc actgttccgg aaatcgatgg agatggccgc cttttccaca tccttatcgt
      781 tggcgttgtg aactacaacg ccgatcacat tcttaccgat cttgtattcc ttgtcatcca
      841 gtaaaatttc gatgctgaac tcagccttct tgctgatatc aaagagagtc tcttggaagt
      901 gtttggtgaa tatgcgctta gcgttcttat cagcttcagc ctggacatac gatgcaagca
      961 cggtgagcag aagacctatt cccaacactg taagtaatgt ctttgggaaa aaaagtttca
     1021 tatgcatctc ctttttgggt ttttggattt tatcttgttg tatgtttatt ttacaccata
     1081 ttgtccaaaa aaataggtgg gaagctgtga caaaatacgg tacaatgagg aaaagaatgg
     1141 cttactattc ctggctgaac atgagaagcc taagaaatgg agcgaaagag ttgaggccat
     1201 ctgaggagca taatcaaaaa caaaggctta atagcacgac tgaaatacta ttcattggaa
     1261 aggagaggat atgagcaaga aagtctttgt gggcgcctta atcttcgtga tagtggtggg
     1321 gggaatttat ttctacacgt tgaccgttgt cttcccttcg ccaaaaaact gcagcgtgtg
     1381 ccatttcatc actccgtttt acaaacagtg gcagacttcc actcacaaca aggttccctg
     1441 cctgaaatgc catgagtatt cacccaccaa ggctctagtg ggtcaattca tgttcttgtc
     1501 cggctcatat aatcctcgtc ctcttaccaa cgtgccggat aagaattgcc tccagtccgg
     1561 atgtcatgat aagaggatga ttgattcaaa ggtgacgttt accaagaggg gcatcacctt
     1621 tgatcacaaa acccatttta cagaaatgaa aagggggata acactccatt gcaggagctg
     1681 ccactccgat atcgtacagg gatcgcatat caaggtttct atgaatgtat gttacctctg
     1741 ccatttcaag ggagtccccc atgatcaggc gctcaccggt tgcccctcct gccattcttc
     1801 tcccacacag cctattgtgg ttatgggaaa aaccttctcc cacgtcgaag cgttaaaggc
     1861 agggtacaaa tgcaatcagt gccacataga agtaacgaag ggtgatggcg tgaccccaaa
     1921 ggacaaatgt tatttctgtc atgtggatag aacggaaaaa tacagtgacg ttaaactcat
     1981 tcataaaaaa catgtaactc agaagcagat agactgcctg tggtgtcata caaagataga
     2041 gcatggaaag atagccatgg cggaaaaaat acctctacgc aggtaatatt tttgctggag
     2101 cgaagaacag aaatggaatt cactaaccca gataatccat aaaagcagga gaggtatccg
     2161 gctcgaacgg caagactcag ggttatatga cggtccgcaa gagcggacct tgacaaaaag
     2221 tgagaacaga atgttcttag atgcgcagag ccaggtatag tcaataaggg ctattgtctt
     2281 gcctggagac ggatgccttt cctataagaa agagactcat gaaaagaatt ggctaagtcg
     2341 aagaggggga cggctttctt tggcgtccct cctgctaaag cttctttccg ctaaagggtg
     2401 cgagcttccg tagtccctcc atgagttttg caaagtcctc gactctcaat gactggggac
     2461 catcgcagag cgccttctgt ggatcggggt gaacttcaac catgatgccg tccgcgccgc
     2521 atgccatggc agcaaaacag agaggggaca cgtaattact tactccagtg gcatgggatg
     2581 gatctacaat gataggcagg tgggaccgtt catgaatcac cggtatcgca gagatatcca
     2641 gtgtgttacg tgtggcattt tcgaaggttc ggatgcccct ttcgcagagg atgacctcct
     2701 tgcagccctc ggagagaata tactccgcag acatgaggaa ttcctgtatg gtagttgaca
     2761 tgccccgttt cagcacaatg ggtttgccga acttgccgac ccttctgaga agcgagaaat
     2821 tctgcacgtt ccttgcccct atctggagca cgtccgcgta tcttcccacc agttccacgt
     2881 cttccggatt gatcacttca gtaacaaagg gaatgccgga ttcttgcctc gcctttgaga
     2941 ggagtttcag gccttcttct tcaaggcctt ggaaggcata gggtgaggtc cttggtttaa
     3001 aagccccgcc cctgagcatt ctggcgcctg cctttttgac ttttatggcg gtctcaataa
     3061 tctgtttttc gctttccact gagcagggcc cagccaccac tccaaaatgt cctccaccaa
     3121 acaaagcact gtctaccttc actatggtgg gttctttctt gatctctttg ctcgcaagct
     3181 tatacggctg gaggatgggg accagctttt ccacacaggg aatcgcctcc agtgcgtaga
     3241 ggaggtgttt gtcacggtca tcgcccacgg ccccaataac cgttctttcg acgccaacga
     3301 ttgcatgggc cctgaaccca aggtccttca ctttttttat gattgactca acggtctttt
     3361 tcttgcagcc cttttttaga acgataatca tatggcctta ctttagaatc ttttattggt
     3421 tcaagtcaat ggaggggcct ttcatgcatt ggggtctcac tgcagaacgg tgctgcggtg
     3481 agtgcttttt gtgaacgaat tgctcttttc caccgggaat aatccttatg aattaaaggg
     3541 aattaattga tgtggcgtgg ctttccgtgt tatagtacat tcacataaat tcagttatat
     3601 acgctttttg aggaggcttc tgcaggcatg ctcatagtcc aaaaatatgg cggcacctct
     3661 gttgcaaatg tggagaggat taaggcggtt gccggtcggg tggtaaaggc tacccaggag
     3721 gggaaccagg ttgtggtggt cgtgtcagct atggcagggg agactgataa gctcatcaac
     3781 cttgcccata aaatttcaca gaacccgtct gatagggaga tggatctcct cctctcttct
     3841 ggtgagcgcg tcacaagcgc cctgaccgcc attgccatcg aatccttggg gcacaaggct
     3901 atggccttca ccggcaggca aatggggatc atcaccgata ccgtgcatac aaaggccagg
     3961 atagagaaga tcacagggga aagggcgaaa aaggtcctgg acgaagggta tgtggtggtg
     4021 gttgctggat ttcaaggagt taccgaacat aaggatgagg tgaccaccct cggaagggga
     4081 ggttctgacc tttctgcagt ggccatcgcc caggcattga atgcagacct ctgcgagatt
     4141 tataccgacg tggacggggt ttacactaca gaccccaata tcgtccctga agcaagaaag
     4201 ctggataaga tctcttatga ggagatgctc gaactggcga gcctgggggc aaaggtgctc
     4261 cagacaaggt ctgtggaatt tgccatgaac tacggggtgc ccgtggtggt caggtcaagc
     4321 ttcaacaata accccggcac gcttgttacc aaggaggata aggatatgga gaaggttgcg
     4381 gtttcagggg ttgcatacga taagaaccag gccaaaatta cggtaacggg tgtcccggac
     4441 aagcccggta tagccgcaaa gctctttaag ggaattgccg atgccaacat gattgtggac
     4501 atgatcgttc agaatgtgag cagtgacggg aaggctgcag acatctcatt caccgtgccc
     4561 aagacggaga gcaaaaaggc actgaaaatg accgaagcga ttgtacggga gctgggcgca
     4621 aaaggggtca cgttaaggga tgatatcgca aagatatcca tcgtgggtgt gggtatgagg
     4681 acccattcag gcgtggcagc aaagatgttt gataccctag cgggccatgg aatcaacatt
     4741 gtaatgatca gcacctctga aataaaggtg tcatgtgtga ttgatgcgaa atacacggag
     4801 ctcgcagtaa gggtcctgca tgatgcgttt gaacttgaaa aggcgtaaag aatgaatctt
     4861 tcgaagggaa tatccggata ctcaataggg ctatgggaaa agcagcgcga aagatagagt
     4921 tatttgatac gaccctgagg gacggggccc agacagagga catatccttt tctgttgagg
     4981 acaagctccg cattgccgag agactggatg agcttggcat ccactacatt gagggaggct
     5041 ggcccggttc caatccaaag gatatagaat tcttcaagaa agtaaagaaa cttaaattga
     5101 ggaactccag ggtcgttgcc tttgggagca cctacaggcc gaaacacaga gtggaggatg
     5161 atgccaatat caaggcactc cttaatgcaa agaccgacat tctcaccatc gtgggaaaga
     5221 cttgggattt tcatgtgaga gaggcgttga ggatcaccct ggaggagaat atcgagatca
     5281 ttcatacaac gattttatat ctcaagaaat atgtagataa ggtcttcttt gatgctgagc
     5341 acttctttga tgggtgcaga gacaacccta ggttttccat gaagtgcgtt ctcgctgccc
     5401 aggatgcggg tgccgactgt atcgtccttt gtgataccaa tggagggact cttcccaatg
     5461 gggtgaggga ggtgacggag aaggtttcgg gagcgctgtc tgcgcccatt ggaatccatg
     5521 cacacaatga tgcggaatgt gcggttgcca attccatcgt cgccgtggag gccggggcat
     5581 cacagatcca ggggaccatg aacgggcttg gtgaacggtg cggaaacgcg aatatctgct
     5641 ccataatccc taaccttcag atcaagctcg gactcaggac agtgactgat gagcaactca
     5701 tgaaattgag ggaggtctcc cgatttgtga acgagatcgc caacctcaga catttcaaaa
     5761 ggcagccctt tgtgggcgac agcgcatttg cccataaagg cggaatccat gtaagtgcca
     5821 ttgtaaaaag gccggagacc tatgaacata tcaggcctga atcggtgggg aattcccaga
     5881 gagtgctcat ttcagacctc gcaggcaaga gcaatatcct gaggaaggct gctgaattcc
     5941 acattaccct ggaccctgat gcgccgcagg tccaggacat tgtgacaaaa ctgaaggacc
     6001 ttgaaaacca gggatttcag tttgaaggtg ccgaggcatc ctttgaactc ctcatgaaaa
     6061 agtctctcgg tcttcacaag acattctttg atcttatcgg ttttcgcgta attgtcgaaa
     6121 agagaaagga gaaagaggaa gcactgagcg aggcgaccat catggtaaag gtgggaggcc
     6181 atattgaaca tactgcggca acaggccagg gcccggtgaa tgccctggac aatgcgctga
     6241 gaaaggccct tgagaaattt tatcctcaac tgaaagaggt aaggcttcat gattacaaag
     6301 tgagggtcct tgctgcaggg aaaggaacct cggccagggt gagggtgctg gtggaatcag
     6361 gggatgagga gatacgatgg ggcaccgtgg gtgtttcgga gaatatcata gaggcatcat
     6421 ggcaggcgct ggttgacagc atcgagtata agcttctgcg gggagaataa gccattagat
     6481 acgtataatt tatctctgga attaaaacca tataaggagg aaggaacaat gaaggtctac
     6541 tacgataagg atgcaaaaat gagtgtgctc aaaggcaaga agatagtcgt tgtggggtac
     6601 ggaagtcagg gacatgctca tgccaacaat cttaaggaga gcggcctgga tgtgacggtg
     6661 gccgagacaa aagggagcaa ctgggaaaag gctgagaaag cggggttcaa agtactcacc
     6721 gccgctgagg ctgcaaaaaa agcggacgtg atcatgatgc tcgttcctga tgaatatgcc
     6781 gcggacatct acaagaccga gatcgccccg cacatcaaga aaggcgctta cctgggattt
     6841 gcccatgggt ttaatattca tttcggccag atagcccctc cgcaggacgt gaatgtcttt
     6901 atggtggcgc caaagggacc gggacacttg gtgagggcag aatatacaaa agggagcggt
     6961 gttccctgtc tgatcgcggt ctttcagaac ccttccaaaa acgcaaagga tgtcgccctt
     7021 gcgtatgcat caggcatagg aggcggacgc gcgggaatca ttgagacctc gttcagggag
     7081 gagacggaaa cggatttgtt tggcgagcag gtagtattgt gcggagggct cacggccctc
     7141 attcaggcag ggtacgagac actggttgaa gcagggtatt cacccgagat ggcatacttt
     7201 gaatgcctcc atgaagtgaa gctcatcgtt gacctcattt acgagggcgg catctctacc
     7261 atgagatatt cgataagcaa taccgcgcag tacggtgacc tgaccagagg accgagggtg
     7321 atcaccgatg aaacgaaaaa agagatgaaa aagatcctca cagaaatcca ggacggagcc
     7381 tttgccaggg aatggttgct ggaatgcaag gtaaacaaac ctgtattcaa tgcattgaca
     7441 cggaagggcg aggaacatcc cattgaacag gtgggggcga agctgagggc aatgatgcca
     7501 tggcttaaga agggcaagct ggtagataaa tcgaaggcct agtggctaag caggagacat
     7561 acagcctcag gatttttata atcgacagga tattgaaatg gcagaacctg gcaccgttag
     7621 gcatgggttt ttctcccgtg ctcaacaggg ttcttttttt aaggtgtcgt ataaaaatcc
     7681 tttgtctgcc tgtctcctgg attacccgtt ttatcttcca tgacctttcc ttgcctgtct
     7741 tgggatgagg atgattaaac tggcccctga aggtaatccc ttcattcttg gtttcctcgg
     7801 tctgacgata ctcgtggatc tcttcgcccc ctggggggcg ctgcttcctt ttgtactcac
     7861 cctttttatg gtctactttt ttagagaccc tgaacggcgc atacctgagg gaaacaatgt
     7921 tcttgtatcg cctgcagatg gaaaggtcat cctgatacag aaagtatctg aagataagtt
     7981 cctcaaagca gacgcggttg aggtgagcat ctttatgtcg cccctggatg ttcatgtaaa
     8041 cagggcgccc tgtgagggag tggtggaaga cgtggtgcat acttccggaa gatttctctc
     8101 tgccttcagg catgaggcat cgctccagaa tgaaaacatc gcgatgcttc ttactacaag
     8161 atacggcaga atcttagtcc ggcaggtggc agggtttctg gcaagaaggg ctgtctgcag
     8221 agtaaagaca ggagactccc tccgcaaagg tgaacggtac ggcatcatca agttcagttc
     8281 gaggctggac atatacctgc caaaggaagt tcagatgagg gtgaaactgg gtgacagggt
     8341 caaggcaggg gagacggtca ttggggaaat gccttcagga cattaagctg tccggcggtt
     8401 tttctgcggt acaggagcaa tcacctgtgc taaagaaacg acggttatta ccttgttgtc
     8461 gaacttgttg ttgtgtgctt tattgttgct attagttata gttggttctg tcattctgta
     8521 tttcttcttc cgctactgtg tcagttatct ctgtgtattc ttccctcact tcttgcaatg
     8581 tgaaggacag tacaaatagc aactcaagaa ctgcgtaagt aacaactgcg acagcactat
     8641 tcaaaactgc taaatctatt gctggtgttg acggtaaaac cttacccact gcaattacca
     8701 caacagcaaa acataggaga taataaacac ctgacgtcgt attaacgtga agtttcgcta
     8761 aaacacgcct aatgaaaagc aaggataact gattcatttc ttgttcgaga gggagtaggt
     8821 atctataaag gagtgtatgc agtcctggag gtgggttatc catcttcagt aacttggtta
     8881 attggtcatc tatgcccttt tcccagttct g
//
LOCUS       DATKOU010000174        19415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1014580, whole genome shotgun sequence.
ACCESSION   DATKOU010000174 DATKOU010000000
VERSION     DATKOU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 19415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19415
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014580"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..713)
                     /locus_tag="VMU21_07910"
     CDS             complement(306..713)
                     /locus_tag="VMU21_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77488.1"
                     /translation="MSNRKTINKKMTNLLTGVFVTVVATLFVMSFAIQVNALESKANL
                     KGEVVAVDDYAKTMTVKSMETVPSSGIGMNDNITFTADKGTYITSCTQNRIFNDINTG
                     AKVAVTYHELDGKLIADVIDIAPVVLAYACYDE"
     gene            1028..1213
                     /locus_tag="VMU21_07915"
     CDS             1028..1213
                     /locus_tag="VMU21_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77489.1"
                     /translation="MVILLCLTLGLAPFHPPHILEKLEMLIKGRLVRPIDWVDLLLHA
                     APWVLLVLKGIVSLRNR"
     gene            1284..1484
                     /locus_tag="VMU21_07920"
     CDS             1284..1484
                     /locus_tag="VMU21_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77490.1"
                     /translation="MAKYTASIDEDTRQRILALPKGVHFSAFMRRAIAVFVEELEKDP
                     GLEPGDFIITRAPGNGSTDKIE"
     gene            1489..2655
                     /locus_tag="VMU21_07925"
     CDS             1489..2655
                     /locus_tag="VMU21_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77491.1"
                     /translation="MEKSRILITCAKGITPFLKEELLQQGFPVLSEGVAGIATEGTFD
                     DTLRLNLLLRTGHRVLYLLRELPARDAEALYRGVSEIPWEKHIAEDGYVCVTSSVESP
                     SIRDSRYANVKCKDAIVDRIKEKRGQRPDSGPDQNRAVVNLYWKDEVCSLYLDTSGEP
                     LSRRGYRKIPLGAPMQETLAAAVIMATGWKGHGHFINPMCGSGTLAIEAALAGLNRPP
                     GLLRDNFGFMHLKEFKEAFWNDLRAQAKREAKKSLQCRIIATDIRREAVEAARKNAAT
                     AGVEHLIEFSSCDYSETSIPEGEGVVILNPEYGERMGKIKELESVYKGIGDFFKQRCK
                     GFKGYLFTGNLDLAKKVGLKAKRRIPFFNGGIECRLLEYELYEGSRKNKNPQIT"
     gene            2689..3498
                     /locus_tag="VMU21_07930"
     CDS             2689..3498
                     /locus_tag="VMU21_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO77492.1"
                     /translation="MCEFCTKHGEGKVWYKNAANYGNDLLSDLNRRTYIENFLSSAFK
                     DGFQTLGRLETIYRKKGRLPRAVESALISKAKEEHFGQVLPIGEISELVRKASTIVRM
                     PCACRWDTEKKEVRCCYAVSYGPEAWYRDIDMSYFGMTPDEGLESLSPDSAIQQMQEM
                     EEQGAIHTIWTMMTPFIGAVCNCAPDECLAMRTLSRIHVETMERAEHVARVDMESCNG
                     CGLCGAVCHFHAIGSATESGRTFARIDPHRCFGCGLCRRACSTGAISLVVR"
     gene            3573..4037
                     /locus_tag="VMU21_07935"
     CDS             3573..4037
                     /locus_tag="VMU21_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77493.1"
                     /translation="MSIRYINDFLYFVIRPDTHKGKDVLIYCSGANVSLFTPITRGRH
                     GIGSSPVVRGLQLVNMGVRDLALSRGATPRALRGNECEGIVPTKDTWYTERLLIENAP
                     ESFPDEMVHYCVNNLLKKIFKASMRELQLPDTLPEANELQLFIETQCRKYGG"
     gene            4052..4489
                     /locus_tag="VMU21_07940"
     CDS             4052..4489
                     /locus_tag="VMU21_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUO77494.1"
                     /translation="MKGKEKKSDLIKKAKTLMKDRDLAEALAAMIEKKRGNLGFLLGE
                     LKERRHRTFTPFVLKGTSIYAEPSALDPKTVELAAVAASSALRCEHCLEAHMERALQE
                     GASLDEILDVILIAGAISESSTLSVSFRKFKQKAGRMQRKESS"
     gene            4523..5869
                     /locus_tag="VMU21_07945"
     CDS             4523..5869
                     /locus_tag="VMU21_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HUO77495.1"
                     /translation="MRKTLTPLLLLFFLSPAFPEEQIPTFTNEDLRKYLTEPLNERET
                     PQQNTNEEALSPPEQKDAAAIIRENRQSIVIIAALDKNGTIYGHGSGFVLSREGAVAT
                     SYHVIRNAADIRIKAGEEVYAAAGIIRADAEHDIAILKAEGSGLHPVRLGRSDSISPG
                     EKVYVIANPRGTGLALSEGILTEIREVAGKGRMLQITAPFTEGYSGSPVFNEQGEVIG
                     MATFIVKGSGDPTAYQKLNFAVPVESIKENASSSSLTPMKDLRTDDYQETSNYWVGLG
                     NRLTGSGKYSEAADAYKRALGIDPSDAEALNGLGVSYMKLKKYKEAIETYKHAIALGS
                     HTAWVYSNLGLAYIEARMYREALEPLTQAVTIMPELEEAHYNLGIAYKNLGMPKEAIA
                     SFKNAISINPGSRDAHFGLGLAFMDVKDKDSALGEYSLLKTLAPSLADMLLKRIRQ"
     gene            6081..6776
                     /locus_tag="VMU21_07950"
     CDS             6081..6776
                     /locus_tag="VMU21_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HUO77496.1"
                     /translation="MKAIILSVSVVVFVLFSVSCSKPDYKKVADDFIKTSTPLKDYTI
                     REVANTNSSLWKAVIVYVKQGRASMPVLLFVSSDGKAVVPNSMVYVNNKPIFTKQLEP
                     ELGRIDFKLTDKDRMVYNAQGKKTVYMFFDPDCPYCKKAKEKLPNYSGEYRVVVKYFP
                     LEQIHPGATLKAIGEQAQWLKKNRKDLTKEADTLKEAKRMVEEDIMEARKAQIEGVPT
                     YVMEDGSIKQGLF"
     gene            6993..8171
                     /locus_tag="VMU21_07955"
     CDS             6993..8171
                     /locus_tag="VMU21_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUO77497.1"
                     /translation="MRKGKGFSGMNRREFLKAGLVGTTSAMLGGNTVAEAIGQYASSE
                     PFVFPKPVYRTLGRTGMKITVVSFGAMLTPEAEVIRTAFEHGVNYVDTARKYMDGKNE
                     GIVAKALKGRRDKVYVATKTLPTSKSKEDIIRDVETSLRTLETDYVDVIQLHNLASKE
                     RIFIPETREALAKLREQGKVRFFGVTTHKNQPEVLNALVDDPDRFFDTALVAYNFKSD
                     NDTKEAIARAAQAKIGIIAMKTQAGGYATDAMGPVSPHQAALKWALQNINITTAIPGM
                     RDMAELREDIAVMGMPFGYADERVLQRYSAEVGPYYCHFCGKCEKTCSKGVEISTINR
                     SLMYAEGYKSPELALSTYREIPVAASASACLDCADCVARCVNGLDIRTKMKRAREVLV
                     "
     gene            8164..9135
                     /locus_tag="VMU21_07960"
     CDS             8164..9135
                     /locus_tag="VMU21_07960"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HUO77498.1"
                     /translation="MFRKRTLDNSHGAQRISLRKYCFISAIILCAVCASAAFCAEESS
                     IEKVLPAPHCAEGWLMEEKVALYTKDTLFERINGESELYFPYGFEALASARYANSKNP
                     EVAVEVDVYKMGSLLDAFGIYANYRRADDGTVRIGAEGFVSPSQLLFYQDRYFVRLQA
                     SGTLSVEQEVFLSCARAVSGNLPRNLSQPRELELFMMPAVKQRSERYISQSLLGYAFF
                     RRGLIADAVLEGEALKVFVVSEDSVNAARKAFDQYREYLKASGQKVQVTETPDKTSVT
                     AVDPLYNNIYIEHFGRYLIGAVNLKKTSAGKQLVEQLRRRLTGDQGS"
     gene            9537..10499
                     /locus_tag="VMU21_07965"
     CDS             9537..10499
                     /locus_tag="VMU21_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77499.1"
                     /translation="MYQIYKNKHFPHFPLSLLAAVILLYFGCALAVEKPHQGEDLLLD
                     AYHKHRAKLEKNDFGFPLYLASSEHSGTLHVDVYGIIDHPFSSVTDMLKVQGNWCDIA
                     SLHPNVKACTYVVFPNESRLTFYIGRKARQSLEDAHQSVYYYKNVEERQGYLDIVLSA
                     AEGPFGTKDHRMRFEAIPLDTGKTFAHVSYTYSAGFPANLAGKIYFATLGRGRTGFTV
                     TGTDGSGNPIYIGGQRGAIERNVVRYYLAIQSFMDTLHSPEESRLTMRLSKWYDYTSR
                     FRKQLYEVEKKDYMEVKTEEYKKQMVLQQRVLPAIQSAPGPSKP"
     gene            complement(10723..10896)
                     /locus_tag="VMU21_07970"
     CDS             complement(10723..10896)
                     /locus_tag="VMU21_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HUO77500.1"
                     /translation="MKVLTCKDVGVDCGYKVRGRTVDEVLKKASEHAKKDHNIKKVTK
                     DYLDSWRTKVHDE"
     gene            complement(10956..11123)
                     /locus_tag="VMU21_07975"
     CDS             complement(10956..11123)
                     /locus_tag="VMU21_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77501.1"
                     /translation="MVLEIDEKEKETLRHALKVLEEELKVERLKADKREFRAALHDEE
                     DSVKRILRKVA"
     gene            complement(11552..13240)
                     /locus_tag="VMU21_07980"
     CDS             complement(11552..13240)
                     /locus_tag="VMU21_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HUO77502.1"
                     /translation="MTQIKLITKEIIVLLLTFVFLCTQALSSERSSSVQISPPGLSLL
                     IKKRIAEGRAPLGTVIGGEQIYAEVLVQSFYKGRNYQPAWSQDGHLIQTESLIMAVEE
                     AYGDGLTPDYYHLGLIRSLVDKVTKEVSPDPTRLADLDILLTDTFLTLGCHLSAGCVN
                     PVTIEAEWFAKRGSVDVSSVLEQALRKKQIREALMRLRPVQASYERLRQVLARYRELS
                     LRGEWPLVSGGSLLKEGSTSDRVAELRKRLSFSGDLAADVAKEGDFFDEKLKQSLIIF
                     QKRHGLKADGVLGSATINALNVPLRQRTRQIELNMERLRWILGNVEQRSIVVNIADFQ
                     LDVMESGKSILSMKVVVGKPYRRTPVFTAKMTHIVINPPWNIPDRIAREEILKKIKND
                     PHYLAEQNIKVLRGWGSRQEEIDPETIDWSEITPKTLPYHFRQEPGPLNPLGRLKFMF
                     PNSFDVYLHDTPAKRLFSENVRTFSHGCTRIEKPIELGEYVMRDNSGWTREVLLNEIE
                     EGTEKKVLISRPLNVHFLYLTAWVDEGGTLQFRNDIYGRDNSLDEALRKKPSLH"
     gene            13385..14731
                     /gene="thrC"
                     /locus_tag="VMU21_07985"
     CDS             13385..14731
                     /gene="thrC"
                     /locus_tag="VMU21_07985"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HUO77503.1"
                     /translation="MPYRAWFQCINEQCKAIYPLNSIIYRCKTCGSLLEVQHDMKALA
                     RRDAKAWMKLFEDRYKSTQWPYGSGVWGKKEWILPKIDDNNIVSLYEGGTNLFWAERF
                     GKIIGLDNLWIKLCGNSHSGSFKDLGMTVLVSMVKQMISEGAPIKAVACASTGDTSAA
                     LAVYCAAAGIQSVVLLPKGKISIAQLVQPIANGALVLSLDTDFDGCMDIVQEITQDET
                     IYLANSMNSLRIEGQKTVGIEIVQQFDWEIPDVIIIPGGNLGNVSALGSGLLMMRDLG
                     LIARLPRIVVAQAERANPLYRSYLKNFETFESIHAEKTLASAIQIGNPVSVQKAIRTL
                     KQFNGIVMDATEQELADAAALGDTTGMFNCPHTGVALAALIKLIKAGKIDRSEHVVVI
                     STAHGLKFTDFKINYHEGTLDFPCQYANKPIDLPARMDAIKEALQQALLKRRNTNV"
     gene            14724..15932
                     /locus_tag="VMU21_07990"
     CDS             14724..15932
                     /locus_tag="VMU21_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUO77504.1"
                     /translation="MSEKKPNAGKWNPATLAIHGLRRVQHAHYAVSMPIVHTSNYYFN
                     DTEEVYEFMKAKSEGRVVREHEYGRYGNPTQEECERKLAAIENAERAVLFSTGMAAVI
                     LTLMTYMRRDGHIIFTNDCYRQTRDFATSLLAEFGLQVSLVDPTAEAIEKAIRPNSNV
                     IFTESPTNPYLRCLDLPAVVKVARQHKVMTVIDATLATPYNIKPLDMGVDIVVHSATK
                     YLGGHNDLLAGVALGKHELLNDLYRMQRMFGATPGPLTCFLLDRGLKTFALRMEHHNA
                     AGLTVARMLESHPKIEKVWYPALSSHPDHNIAREQMKGFGSVITFLLRGGDAETRRFI
                     DALELFLITPSLGGSESLVTQMSTMSFFDYPADYRRQIGMVDNLVRLALGLEDVDDLI
                     TDLRQALDKI"
     gene            complement(16441..19392)
                     /locus_tag="VMU21_07995"
     CDS             complement(16441..19392)
                     /locus_tag="VMU21_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HUO77505.1"
                     /translation="MISLQGLTKPFKDSLETSVEIRVNDFDIAHYLAYMPAEMDFKVL
                     SGRLDANTLLFFTQYKNRAPSITLTGSLQFKDIRIVDKKESPLIHLPLLDISVDSGEL
                     MSRRVLLSKVLLQSPEVHVVRDKTGRVNLQSLIPKKGYVLKRKEESPVLSLYADEIDL
                     TNGTVTFSDLLQEETFRSTLENIQVKIDRFSNARDWKSAITLSLGTEAEEGVKVSGEF
                     TVDPLSSQGRAELAKIPLKKYSPYYRRQILFDIMDGVLDFSTEYAFQQTEKEQAVRLS
                     DLSAHLTSLSLKKRDEKDEFLRIPEIAMKEAAIDLQKREVVVGEFSTEKGFLMVKRGE
                     DGKWNLQTLLPPTPDAKKPAAKQNQNERPWTVTMKKISAQGYGVKGEDLVPTQPVSFS
                     AEIIKLAAEGISTEKNKSGKVSLSLKFGKKGSVSAVGSVVLNPGSANVRLNCNKVDIV
                     PFQGYLSDRVKIVLAEGDFSTSGALTLGYGERGSPKVSYKGELFLAKFWSLDKSNAED
                     FLKWDSLHFGNLRAGYNPFFLNIDDVAMANFYSRIIINEDGSLNVQDVSGAEEANGAV
                     AGENATTTKIPPPENKKAPDKTVTVEKVTLQGGTIDFLDRHVKPNVSANLTEVGGRIS
                     GLSSEENKFADVDLKAKYDNYAPLEITGKINPLREDLYVELKAAFKDMDLSPMSPYGG
                     KYMGYTIEKGKLSLNVEYLVVKRKLTAQNNLYLDQFTLGDKIESPDATKLPVKLAIAL
                     LKNRKGEISLDLPVTGNLDDPKFSLGRIIIKILLNILVKAATSPFALLGAIFGGGEEL
                     SYIEFDYGSASLNEQGLKKLDTLVKALYDRPALRLDIGGYADPEKDGEALREHLFKGK
                     IKAQKLKEMVKKGSTPVPLEEVIVSAEEYPKYLKMAYKEEKFPKPRNIIGIAKDLPVP
                     EMEKLMLTHTQVNDDDLRHLASERALKVKDHILKSGKVEQERIFLVESKALQAEKKEK
                     VKESRVDFKLK"
BASE COUNT         4920 a         4801 c         4883 g         4811 t
ORIGIN      
        1 acctttgtta taccctccca gaggtttccg agtcaagagg agcattgagc taaaaagcgt
       61 tgattcctaa ttgaaagcta tggccgatga cgagttccag aagagaaaga ttacaggaag
      121 aaaatctcat tcaatgtttt agggtgaaag cagtattgcc aggtttagag gggtatccgg
      181 aaacatacag gataaatgtc ctcaatagcg tggaatgtgc atatatcact ttgctgaatt
      241 gcggaggcaa agaaaaagcg ccgtacaatt gcggcgctct ctttttcact caatgcggtt
      301 cttcattatt cgtcataaca cgcgtaggca agcacaactg gcgcgatgtc aataacatca
      361 gcgataagtt tgccatccaa ctcgtgataa gttacggcta cctttgcacc ggtattgatg
      421 tcattgaata tcctgttctg ggtacagctt gttatgtatg tccctttatc cgctgtgaat
      481 gtaatgttat cattcattcc aatgcccgaa gaggggaccg tttccataga ctttaccgtc
      541 attgtttttg catagtcgtc aactgctacc acctctcctt taaggttggc cttgctttcc
      601 agggcgttga cctgaattgc gaaagacatt acgaaaagcg ttgcaacaac tgtaacgaaa
      661 acacctgtca ggagattcgt cattttctta tttatggttt ttctgttaga catgatctgt
      721 acccctttct tccttgtact ttactactac ttgcaaaacc taaggttctg catccttctt
      781 gaatacatca tataactaat tgcagatata tgcaatcttg ctactaagcg ttatagttca
      841 atcctgggcg gaatactggc ataaaaatca taaaagcacc ctttttcaag gacttatttc
      901 ttctttgggg tattaagctc attttttgct gtcctttatt gtgagttcca gtgtctgaat
      961 aaagcgtaat cttcgcctgt ggtatacttt caccgtggaa cggtggttat cgcacgtatc
     1021 atggccagtg gtcatccttc tctgtctgac actcggcctt gcgccctttc atccgcccca
     1081 tattctggaa aaactcgaga tgctcataaa gggccgcctt gtgcggccta tagactgggt
     1141 tgacctcttg ctgcacgccg caccctgggt gcttctcgtc cttaagggga tagtgtccct
     1201 aagaaacaga taggggcacc aagagtgttc aatgataaag aggagaaatt cgcgcttacc
     1261 atttcatcac ataaggagtg gatatggcaa agtacacagc aagcattgac gaagatacgc
     1321 gccagaggat ccttgcttta cccaagggcg tacacttctc agcctttatg cgaagggcga
     1381 ttgccgtctt tgttgaagaa ctcgagaagg accctggcct tgaacccgga gatttcatca
     1441 ttacccgcgc acccggaaac ggttcaacag acaaaataga atagaggcat ggagaaaagc
     1501 aggattctca ttacctgtgc aaaggggata accccttttt tgaaggagga actcctccaa
     1561 cagggatttc ccgtgttgtc cgaaggtgtt gcaggtattg ccacggaggg aacttttgat
     1621 gataccctca ggctgaatct ccttctccgc acaggacacc gcgtcctcta tctcctcagg
     1681 gagcttcctg cccgtgatgc tgaggctctc tatcgcgggg tgtcggaaat cccgtgggaa
     1741 aaacatattg ctgaggacgg ctatgtgtgc gtgacctcca gtgtggaaag tccctccatc
     1801 cgggactcac gctatgcgaa tgtgaagtgc aaggatgcca ttgtggacag gatcaaagaa
     1861 aagcgaggac agcgtcctga ttccggtcct gaccagaaca gggctgtggt gaacctctac
     1921 tggaaggatg aggtctgctc cctgtatctt gatacctcgg gtgaaccact ctcccggcgc
     1981 ggatacagaa agatacctct gggagcgccc atgcaggaga cccttgcagc tgccgtaatt
     2041 atggcgaccg ggtggaaggg ccatggtcat ttcatcaatc ccatgtgcgg cagcggtacg
     2101 cttgccatag aggctgccct ggcaggcctg aacaggcccc ctgggctctt aagggacaat
     2161 ttcgggttta tgcatctcaa ggaatttaag gaagcttttt ggaacgattt acgtgcacag
     2221 gcgaaaagag aggcaaaaaa gagtctgcaa tgcagaatca ttgccactga tatcaggagg
     2281 gaggcagtag aggcggcaag gaagaatgcg gcaactgcgg gtgtggaaca cctgattgaa
     2341 ttctcatcat gtgattattc cgaaacgtct atccctgaag gggaaggagt agtaatcctc
     2401 aaccctgaat atggtgaaag gatgggcaag ataaaagagc ttgagagtgt gtacaaaggc
     2461 atcggtgatt tcttcaagca gaggtgcaag gggtttaagg gatatctttt taccggaaat
     2521 cttgatctgg caaagaaggt agggttgaag gcgaaacgga ggatcccttt ttttaacggt
     2581 ggcatagaat gcagactcct tgagtatgaa ctgtatgagg gcagcaggaa gaataaaaac
     2641 ccgcagataa cgtaaaataa caaaaacgca tcaaccaccg gagaagagat gtgtgagttc
     2701 tgtacaaaac acggagaagg gaaggtatgg tacaagaatg ctgccaatta tggcaacgac
     2761 ctgttatctg acctgaaccg ccgcacctat atagaaaact ttttgtcttc tgcgtttaag
     2821 gacggtttcc agacactggg aagacttgag accatctaca ggaaaaaagg ccgtctcccg
     2881 agagcggtgg aaagcgccct catcagtaag gcaaaggaag agcacttcgg tcaggtgttg
     2941 cccattggag aaatcagcga actggtaaga aaggccagca ccattgtccg catgccctgt
     3001 gcctgccgct gggatactga gaagaaagag gtgcgctgct gctatgcagt cagctacggc
     3061 cctgaggcat ggtacagaga catagacatg agttatttcg gcatgacacc ggatgaaggt
     3121 ctcgagtccc tgagtcccga tagtgccata cagcagatgc aagagatgga agagcagggg
     3181 gcgatccata ccatctggac catgatgacc ccctttatcg gcgcagtctg caactgtgct
     3241 cccgatgaat gtcttgccat gaggacactt tcccggattc acgtggagac catggaacgg
     3301 gcagagcatg tggcgagagt tgacatggag tcatgcaacg ggtgcggcct ctgcggcgca
     3361 gtctgccatt ttcatgcaat cggcagtgcc acagagagcg gcaggacctt tgcccggatc
     3421 gatccgcacc gctgctttgg ctgcgggtta tgccgcaggg cgtgcagcac cggagcaatt
     3481 tccctggtgg tacgctgacg ctcaaatgtg gcacactgcg atcaccaggt gggacgcaac
     3541 gcaggaactc aactggtcga ggagggaata ctatgtccat ccgctatata aatgactttc
     3601 tttatttcgt gatacgccct gacacacata aaggcaaaga tgtactgatc tattgttcgg
     3661 gcgcgaacgt atcccttttt acccccatca ccaggggacg gcatggaatc ggttcgagtc
     3721 ctgtcgtgag ggggcttcaa ctggtgaata tgggcgtgag ggaccttgca ctttcaagag
     3781 gggcaactcc cagggctctc cgggggaatg agtgcgaggg gattgttccc acaaaggaca
     3841 cctggtatac tgaacggctg ctgatagaga atgctccgga atcttttcct gatgagatgg
     3901 tacattactg cgtgaacaat cttctaaaga aaatcttcaa agccagcatg cgggaactcc
     3961 aactacctga cactctccct gaggcgaatg aacttcagct gttcatcgag acccagtgca
     4021 ggaaatacgg aggataattg acaagaaggg tatgaaaggt aaagaaaaaa agagcgacct
     4081 cataaagaag gcaaagacat tgatgaaaga cagagacctc gctgaagcgc tggccgccat
     4141 gatcgagaag aaacgcggca acctcggttt tctgcttggc gaactcaagg aaaggaggca
     4201 ccggacgttt accccctttg tccttaaggg aacctccatc tatgccgagc cctctgccct
     4261 ggatccgaag accgttgaac tcgctgcagt ggctgcgtct tcggccctca ggtgtgaaca
     4321 ttgtcttgaa gcccatatgg aacgggcgtt gcaggaggga gcgtccctgg atgaaatact
     4381 ggacgtgatc cttatcgccg gcgcgatttc ggaatcctcc actttgtcgg tctcattccg
     4441 caagttcaag cagaaggcgg gcaggatgca gagaaaggaa tcaagttaag aatacggcct
     4501 gcggaaaaag gctctggaga cggtgagaaa aacattgaca ccacttctgc ttctcttttt
     4561 tctctcccct gcattccctg aggagcaaat cccaactttt accaatgagg atctcaggaa
     4621 gtacctcacc gagcctctga atgagcgtga aacccctcaa cagaatacga atgaagaagc
     4681 cctttcccca ccagaacaaa aagatgcagc tgctatcatt agggaaaata ggcagtccat
     4741 tgtgattata gccgcgcttg acaagaatgg caccatctac ggccacggga gcgggtttgt
     4801 attgagcagg gaaggggcgg tcgcaacgag ctaccatgtc ataagaaatg cagccgatat
     4861 acgcataaag gcaggggagg aggtgtacgc agctgcagga atcatccgtg ctgatgcgga
     4921 acatgatatc gcaatactaa aggcggaagg gagtggcttg cacccggtaa ggctcgggag
     4981 gtccgacagc atatccccag gagaaaaagt gtatgtcatc gcaaaccctc gtggaacggg
     5041 actcgcgttg tcagaaggga tactgacaga gataagagag gttgcaggca aaggaagaat
     5101 gctccagata acggcgccct ttaccgaagg ctacagcggc agtcctgtat ttaacgaaca
     5161 gggagaggta atcggcatgg cgacctttat tgtcaaaggc tcaggagacc ctaccgctta
     5221 ccagaaactg aactttgcgg taccggtgga gagcataaaa gagaatgcct catcctcaag
     5281 ccttactccg atgaaagact tgcggacaga cgactaccag gagacttcca attactgggt
     5341 tggccttggg aacaggctca ctggttcagg aaaatattct gaggctgcgg atgcctacaa
     5401 aagagcatta ggcatcgatc cctctgatgc agaagcactc aacggcctag gtgtttctta
     5461 catgaaactg aagaaatata aagaggcgat agaaacttac aagcatgcca ttgctcttgg
     5521 gtctcacacg gcatgggttt attcaaacct cggccttgcc tatatcgaag caagaatgta
     5581 cagagaggcc ctggagccgc tcacgcaggc tgtgaccatc atgcctgagc tggaagaagc
     5641 acattacaac ctgggtattg cttataaaaa tctgggcatg ccgaaggagg ctatcgcatc
     5701 gttcaagaac gctatcagta taaatcccgg ctcccgggat gctcatttcg gccttgggct
     5761 ggcgtttatg gatgtaaaag acaaagactc ggcattaggc gagtattccc tgctgaagac
     5821 gcttgcccct tccctggcgg acatgctgtt gaagaggatc agacaataaa caaaaaaaac
     5881 accattcatt ccctgttata gttttgttaa agtgcatgcc gagacgaata cgcgtaaaag
     5941 gaagacagat ggttctattt ccacggggtg tattcatagt gggtaatgca ggtaggccaa
     6001 cttgaaaatc ggtatctgtt atgctactct tcaacctgtg tgaaaggaac aatccaatag
     6061 gtcacataag gagaaaaaca gtgaaagcaa ttatcctgtc agtgtcagta gtggtgttcg
     6121 tattgttcag cgtgtcgtgt tccaaaccgg attacaagaa ggttgcggat gatttcataa
     6181 agacctcaac ccctctcaag gactatacca tcagggaagt agcaaataca aactcctctc
     6241 tctggaaagc ggttattgtg tacgtcaagc agggaagggc gagcatgccg gtcctccttt
     6301 ttgtatccag tgatgggaag gcagttgtgc cgaattccat ggtctatgtg aacaacaaac
     6361 cgatatttac gaaacaactc gagcctgagc ttggccggat agatttcaaa ctcacggaca
     6421 aagacaggat ggtgtataac gctcagggaa agaaaacggt ttacatgttt tttgatcctg
     6481 actgccctta ctgcaaaaag gctaaggaga aactcccgaa ctacagtggc gaatacaggg
     6541 tggtggtgaa gtatttccct ctggagcaga tccatccggg agcgaccctg aaagctattg
     6601 gtgagcaggc acagtggctt aagaaaaaca ggaaagacct tacgaaagag gcggataccc
     6661 tcaaggaagc caaaaggatg gtcgaagaag acattatgga agcccggaaa gcacagatag
     6721 agggggtccc cacttacgtg atggaagacg ggagcattaa gcaagggctg ttttagtctc
     6781 tctgttcaac gatgaccaaa caggggtgcc aatgcacccc tgtcgcgttt tttctttagt
     6841 tggcccatct tcgtatcctt tgtcctgttt cgtgagaagg aaactgggca gagccctgaa
     6901 aaaacataat ggtgtttagg gtgttttcat atataatttt gataagggat tggatgctgc
     6961 cataactatg gttttgtcat aaggaggctg ctatgcgcaa aggaaaggga ttctctggaa
     7021 tgaacaggag ggagttttta aaggctggtc tggtagggac cacatcggcc atgcttgggg
     7081 ggaataccgt tgcagaggcc attgggcagt atgcatcatc tgagcctttt gtcttcccca
     7141 aacctgtcta ccggaccctt ggcagaacag ggatgaagat cacagtcgtg agctttgggg
     7201 cgatgctcac acctgaggca gaggtgatac ggaccgcttt tgaacatggc gtgaactacg
     7261 tggatacggc gagaaaatat atggacggca aaaacgaggg aattgttgcc aaggcactga
     7321 agggcaggcg tgacaaggtg tatgtggcaa cgaagaccct gcctacttcc aagtcaaaag
     7381 aagacatcat aagggatgtg gagacaagcc tcagaacact ggagaccgac tatgtggatg
     7441 tcatccaact ccataatctg gctagcaagg agaggatatt cattcccgag acacgggagg
     7501 cccttgccaa actgagagag cagggcaagg tacgcttctt cggcgtcacc acgcacaaga
     7561 accagccaga ggtcctcaac gcccttgtgg acgatcctga ccgcttcttt gacacagcgc
     7621 ttgtggcata caactttaaa agcgataatg acacaaaaga ggcgattgca agggcagccc
     7681 aggcaaaaat cgggatcatt gcgatgaaga cccaggcagg tgggtacgcc accgacgcga
     7741 tggggcccgt cagtcctcac caggcagctc tcaagtgggc gctccagaat ataaatatca
     7801 ccaccgcgat tccgggcatg agagacatgg ctgaactcag ggaagacatc gcggttatgg
     7861 ggatgccttt cgggtatgcc gatgagcgtg tcctacaaag gtacagcgct gaggtggggc
     7921 cctactactg ccatttctgc ggaaaatgcg agaaaacctg ctcaaagggt gtggaaatca
     7981 gtaccatcaa ccgttccctt atgtatgcag aagggtacaa gagccctgaa ctcgctcttt
     8041 ccacatacag ggagatccct gtggcagcct ctgcctcggc atgccttgat tgtgcggact
     8101 gcgtggcacg gtgtgtcaac ggacttgata ttagaacgaa aatgaaacgg gccagggaag
     8161 tccttgttta ggaaacgcac gctcgataat tcccatggcg cacaaagaat atcactgagg
     8221 aaatactgtt tcatctccgc aataatctta tgcgcagtat gcgcctctgc agcgttctgt
     8281 gctgaggaga gttccatcga gaaagtgctt cccgcaccgc attgcgctga aggctggctc
     8341 atggaggaga aagtggccct gtataccaag gataccctgt ttgaacgaat caatggtgaa
     8401 tcagagctct acttccccta tgggttcgaa gcgcttgcct cggcacgata tgcgaacagc
     8461 aagaatcccg aggtagcggt agaggtcgac gtatacaaga tgggctctct tctggacgct
     8521 tttgggatat atgccaatta ccggagggcg gatgatggta cagtaaggat tggcgccgag
     8581 ggatttgtat caccatctca gctgttgttc tatcaggaca ggtattttgt gaggctccag
     8641 gcgtcaggca cgttgagtgt ggaacaagag gtattcctct cgtgtgctcg tgctgtttcg
     8701 gggaatctcc cgcgcaattt aagccagccc agagagcttg agttattcat gatgcctgct
     8761 gtaaagcaga ggagcgaacg gtatatttcc cagagccttc tcgggtatgc ctttttccgc
     8821 aggggtctca tcgctgacgc agttctggaa ggtgaagcgc ttaaggtctt cgtggtgtct
     8881 gaggactctg tgaatgctgc gcgaaaggct tttgatcagt accgtgagta tctgaaggca
     8941 tcggggcaga aggttcaggt gaccgagacg ccggacaaga catccgttac agcggtggat
     9001 ccgctgtaca ataatatcta tatagagcat tttggccgtt acctcatcgg tgcggtgaac
     9061 ctcaagaaaa cttctgcagg aaaacagctc gtggagcagc tgcgcagacg gcttaccggg
     9121 gatcagggca gctgacaagg taggcatttt gcacacccct taatcagcac aaccggaaca
     9181 ttcgctccgc tgcatgaaca tcttttgatg gtatcagcaa agatcctttt tctcttgaaa
     9241 ccaactatcc gcctgcttct ttttgataaa accgtgtaaa atacacccat ggataaaaaa
     9301 gcgctccaaa agaatccatg tcctgactgc accttctgtc agtactgctg tgattcacgc
     9361 tgcgccatgt gccgctctcc gtatcccagg aaaagagaga aagccaagaa acctaagatt
     9421 cataaaccgc tttttctcaa atactaatca cccctgctgg agcgtatttt ttcttgccct
     9481 ccggagttga cttcaggcaa aaaacagggg tattcactat aataaagacg ctccatatgt
     9541 atcagatata caaaaataag cactttccgc actttcccct gtctctcctg gctgcggtca
     9601 ttcttctcta tttcggttgc gcattggctg tggaaaaacc acatcagggg gaggaccttc
     9661 tccttgatgc ctatcacaag caccgggcca aactggaaaa gaatgacttc gggttccccc
     9721 tttatctggc gtcttccgag cattcaggca cactgcatgt ggatgtctac gggattatcg
     9781 accatccttt cagcagcgtt accgatatgc tcaaggttca gggaaactgg tgcgatattg
     9841 catccctcca tcccaatgtc aaggcctgca cctacgtggt attccccaac gagtcgcggc
     9901 taaccttcta tataggtcgc aaggcccgtc aatccctgga agacgcgcat caatccgttt
     9961 actattacaa gaatgtggaa gagcggcagg gttatctgga tattgttctg agtgctgccg
    10021 aaggcccttt tggaacaaaa gatcacagaa tgagatttga ggcaattccc ttggacactg
    10081 ggaaaacctt tgcccatgtg agttacacat acagcgctgg cttccctgcc aacctggcag
    10141 gcaagatcta ctttgcaaca ttgggcaggg gaagaacggg gtttacagtg accggaactg
    10201 acgggagcgg taaccccatc tatatcggag ggcagcgtgg agcaatcgag cgcaacgttg
    10261 tccggtacta tctcgccata cagtccttta tggacaccct gcactctccc gaagagagcc
    10321 gccttaccat gaggctcagc aagtggtatg attatacgag ccggttcagg aaacagctct
    10381 acgaggtgga gaagaaggac tacatggaag tcaagaccga ggagtataag aagcagatgg
    10441 tcctccagca gcgggtcttg cctgcaattc aatcagcacc cggaccctcc aagccttagg
    10501 aatcacacta tactttcaca gggtttccct caccgctggt tcgggacggt cgttttccgg
    10561 ttcggaagtc tgaaaggcgg ggaaatcgag gaattaatac tctccatatg tcgcactttc
    10621 cccctgggtc ggttggtatt gagataagcg gaggggcaaa aaaaaacggc ataaacgaaa
    10681 aagggtcatt tcatgccgtt tagatatata acttcgggag gttcattcat catgaacctt
    10741 ggtgcgccat gaatcaagat agtcttttgt gacctttttt atgttgtggt ctttttttgc
    10801 atgttcagac gcctttttga gaacctcatc tactgttctc ccgcgcacct tatagccaca
    10861 gtcaacccct acatctttgc atgtcaggac tttcatggca tcctcctttc cgcgtggtca
    10921 gaaaattaat tcttaattca ccacacggtt tgcggtcagg ctaccttcct gaggattctc
    10981 tttacagaat cctcttcgtc atgaagtgca gccctaaact cacgcttatc ggcttttaac
    11041 ctttcgacct tcagttcttc ttcaagaact ttaagggcgt gccttaatgt ttccttttct
    11101 ttctcatcta tttccaatac catactctca cctcggttcc tccctcaaag ctaatctgac
    11161 cctttcaggg aagagaaaag atattctcta tcttccctct tctatttatt atattacaaa
    11221 taatatattt tcgcaacaac gaaactaagt cgcaatttcc tcttttggtt tctagtaggc
    11281 ggaaaaggac ataaatctgc accgcccgac gaaaattcag ctttcttccc tcttacttgc
    11341 tttatctcca gatcacgaag atgccatttt atattttatg taagacctgt ttttgggaat
    11401 aagggattca gacactggac aggggaataa gagcttgttt ttgttatggc cgttagtttc
    11461 aaagttctat gactgagctc cgccctgggc tgactgcctg aacgtctcac ctgatgcagc
    11521 gcatattgcg gtggggtaac attgttgacc gtcagtgaag agacggtttc ttgcgaaggg
    11581 cctcatcgag actattgtcc cttccgtaga tgtcatttct gaattgaaga gtgcccccct
    11641 catcaaccca agccgtgagg taaaggaaat ggacatttaa tggacgagaa ataaggactt
    11701 ttttctcagt accttcctct atttcattca ataacacttc ccgcgtccag ccagagttgt
    11761 cccgcatcac atattccccc agttcgatcg gcttttcaat gcgggtgcac ccatgactga
    11821 aggtgcgtac attctccgaa aacagacgct tggcaggagt gtcatggagg tagacatcaa
    11881 atgaattggg aaacataaac ttgagacgtc caaggggatt cagagggccg ggctcctgac
    11941 gaaaatggta tggtaatgtt tttggggtga tctccgacca gtcgattgtc tccgggtcta
    12001 tttcctcttg tcgggatccc cagcccctta gaaccttgat gttttgttcg gcaaggtaat
    12061 gaggatcatt cttgatcttc ttcagtatct cttcccgtgc aattctgtcc gggatattcc
    12121 acggaggatt tattacgatg tgcgtcatct ttgcggtgaa gacaggggtg cgccggtaag
    12181 gcttccctac taccactttc attgagagaa tcgacttccc gctctccata acatccagct
    12241 gaaaatctgc gatgtttaca acgatggaac gctgctccac attaccaagt atccagcgca
    12301 gcctttccat gttcaattcg atctggcgtg tccgttgcct taagggaaca ttgagggcat
    12361 tgatagttgc agagccaagg acaccatcgg ctttaagccc gtgccgtttc tgaaagatga
    12421 tgagcgactg tttcagcttt tcatcaaaaa aatccccttc ttttgccaca tcagccgcaa
    12481 gatcaccgga aaaggaaagc ctctttctca gttctgcaac cctgtccgac gtggaacctt
    12541 ctttcaagag ggaacctcct gaaaccagcg gccattcacc tcgtaatgat agttctctgt
    12601 atcgggcgag gacctgtctg agtctttcat aggaggcttg tacggggcgg agcctcatca
    12661 aagcctctct tatctgtttc ttcctgagtg cttgctcaag gacagaggag acatccacac
    12721 ttccacgctt tgcaaaccat tccgcctcaa tagtcactgg gtttacgcac ccggctgaca
    12781 ggtggcagcc gagagtgaga aatgtatctg tgaggaggat atcgagatcg gcaagacgtg
    12841 ttgggtcagg agatacctct ttcgtcacct tgtcgacaag agatcgtatg aggccgaggt
    12901 gatagtagtc cggcgtcagc ccatcaccat aggcttcctc cactgccatg atgagtgact
    12961 ctgtctggat caaatgacca tcctggctcc acgcaggctg gtagtttctt cctttataga
    13021 agctttgcac aaggacttcg gcgtatatct gctctccacc aataaccgtt cctaaggggg
    13081 ctctcccttc ggctatcctc ttctttatga gaaggctaag ccctggaggc gatatctgaa
    13141 cagacgagct tctttcagaa gaaagagcct gggtgcataa aaagacaaat gtgagaagga
    13201 gaacaataat ttccttggtt atcaatttga tctgtgtcat ctttttatat tttaccaaat
    13261 ccgggattta ttaagatttt tggctttccc ctcttcttca ttgattcccc ctcggagttg
    13321 aataacggcg tattattatg ataatcttcc cttatatcag aacaaattca ctttaagggt
    13381 accaatgcca taccgagcct ggtttcaatg tattaacgag caatgtaaag cgatctatcc
    13441 gctgaactcc atcatctacc gctgcaagac ctgcggatca ctgctggaag tgcagcacga
    13501 catgaaggcc ttggcccgcc gcgacgccaa ggcatggatg aaactctttg aggaccgata
    13561 caagtccacc cagtggcctt acggctcagg cgtgtggggc aaaaaggaat ggattctccc
    13621 gaagatcgat gacaacaaca ttgtctcact ctatgaaggc ggcaccaacc tgttttgggc
    13681 agagcgcttc ggcaagataa tcggccttga caacctctgg atcaagctct gtggcaattc
    13741 ccacagtggc tcttttaagg acctggggat gacggtgctc gtctctatgg ttaagcaaat
    13801 gatcagcgag ggagcaccga tcaaggcagt ggcctgcgcc tccacgggcg acacctcggc
    13861 agcgttggca gtctattgtg ctgctgccgg catccagtct gttgtgctcc tgcccaaagg
    13921 caagatatcc atcgcccagc tggtccagcc cattgccaat ggtgcgctgg tgctgtccct
    13981 ggatacggat tttgacggat gcatggacat tgttcaggag atcacccagg acgagacgat
    14041 ctaccttgca aattccatga actcgctacg cattgagggg cagaagaccg tgggcatcga
    14101 gatcgtccag cagttcgatt gggaaatccc ggatgtgatt attatcccag gcggtaattt
    14161 ggggaatgtc tcggccctgg gcagcggcct gctcatgatg cgcgacctgg gccttattgc
    14221 caggctcccg aggatagtgg ttgcccaggc cgaacgcgcc aatccactct accgctccta
    14281 tctcaagaac ttcgagacct ttgagtctat tcatgctgag aaaacgctgg ccagcgcgat
    14341 tcagatcggg aacccggtga gcgtccagaa ggcgatacgc acattgaagc agttcaacgg
    14401 cattgtcatg gacgccacag agcaggaact ggccgacgct gccgccctcg gcgatactac
    14461 cggcatgttc aactgccctc acacgggcgt ggctctggca gcgctgataa aactgatcaa
    14521 ggccgggaag atagatagat cagaacatgt tgtggtcatc tcaactgcgc atggtctcaa
    14581 gttcaccgat ttcaagatca actatcatga ggggacgctg gacttcccct gccaatatgc
    14641 caacaaacct atcgatttgc ccgcgcgcat ggatgctatc aaggaggccc tgcagcaggc
    14701 cctactgaag aggaggaata cgaatgtctg agaagaagcc aaacgccggg aagtggaatc
    14761 cggcaaccct cgctatccat ggcctgagac gtgttcagca cgcacactac gcggtctcca
    14821 tgcccattgt gcatacctcc aactactact ttaacgacac tgaagaggta tacgagttca
    14881 tgaaggccaa gagcgagggt agggtagtgc gcgagcacga atacggtcgc tacggcaatc
    14941 cgacccagga agagtgcgaa cgcaagctcg ccgctattga gaacgccgag cgagcggtgc
    15001 tgttttccac aggtatggct gccgtaatac tgaccctgat gacctatatg cgccgcgacg
    15061 gacacatcat cttcaccaat gactgctacc gccaaacccg tgactttgcc acaagcctcc
    15121 ttgcagagtt cggcctgcag gtctcgctgg tggaccccac tgcagaggcc atcgaaaaag
    15181 cgatccggcc caactccaat gttatcttta ctgagtcgcc cacgaatccg tatctgcgat
    15241 gtctcgacct tcctgcggtg gtgaaggtag caagacagca caaggtgatg acggttattg
    15301 acgctaccct ggcaacgcct tacaacatca agcccctgga catgggcgtg gacatcgtgg
    15361 ttcattccgc caccaagtac ctcggcgggc ataacgatct gctggcaggc gttgctctcg
    15421 gcaagcatga actgctgaat gacctctacc ggatgcagcg gatgttcggt gctactccgg
    15481 gacccctcac ctgttttttg ctggataggg ggctaaagac ctttgcactc cgcatggagc
    15541 atcacaatgc cgcgggactg acagtggcgc gcatgctgga atcacaccct aagatcgaga
    15601 aggtatggta tcctgctttg tcatcccatc ctgaccacaa cattgcacgt gagcagatga
    15661 agggtttcgg cagtgtgatc accttccttc tgaggggcgg cgatgcggag acgaggagat
    15721 tcatcgatgc actggagctg ttcctcatta cccccagcct cggcgggagc gaaagcctgg
    15781 tgacgcagat gtcgaccatg tcgtttttcg actatcctgc tgactatcgc cgtcagatcg
    15841 ggatggtgga caatctggta aggctcgccc ttggccttga agacgtggac gacctcatta
    15901 cagacctgag acaggcgtta gacaaaatct gaacaaggaa acttacgcac cccaaatgtt
    15961 gtcccgctgg catctgtgac tttgaagcat cttcttcact ttagcaagga taatgcttca
    16021 aacttttttg agtgctctaa gttccttcta agccctttga ttttcctaat acaccacagc
    16081 tattagatat cgcaaccgat gcgtagtttg gtttgaaact gtcaaaatcc ctttttcgtt
    16141 ttcttcctgc agccaacact gatgcttttc taccacatgg caatgtttct ccaagaatta
    16201 atgggtggca ggagtccggt caattaagct ttcatgtagt tagctccaga attgttttgg
    16261 cgacagcaat tggcagacct ttgtttgagg tctcaaaaat ggttttttaa gatcgtacca
    16321 tctcgaatcg agagtgttgc accgcaccct gtgtggaagg actacgcatt tttggccgca
    16381 ctggcctgtg aagacaatga tactgtgtcg aaaatcaagc acctgccgtg gcacatcact
    16441 tcacttcagc ttgaaatcca cgcggctctc ctttactttt tccttcttct cagcctgaag
    16501 ggcctttgat tccactagga atatccgttc ctgttccacc tttccagact tcaggatatg
    16561 gtccttcacc ttgagggcac gctctgaagc caggtgccgt agatcatcgt cattcacttg
    16621 ggtatgagta agcatgagct tttccatctc tggaacaggc aggtccttag ctatcccgat
    16681 gatatttctg ggcttaggaa atttttcttc cttgtaagcc atcttaaggt atttcggata
    16741 ttcctctgca ctcactatca cttcttccag gggcacggga gtcgatcctt ttttgaccat
    16801 ttccttcagt ttctgtgcct tgatctttcc cttgaagaga tgctcgcgga gtgcctctcc
    16861 gtccttctca ggatccgcat atcctccgat atccagtctc aacgcaggcc tgtcgtatag
    16921 ggccttgacc agcgtatcca gtttctttag cccctgctca ttcagcgaag cgctcccata
    16981 atcgaactcg atgtagctca gttcctcccc tcctccgaag atcgctccca ggagggcaaa
    17041 aggagaggtg gcagccttca caaggatgtt caacagtatc ttgatgatta ttctgcccag
    17101 gctgaatttc gggtcatcaa ggttcccggt aaccgggagg tcgagactga tctccccctt
    17161 tctgttcttg aggagggcga tggcaagttt cacagggagt ttagtagcat cggggctttc
    17221 gatcttatct cccaacgtga actggtctag ataaagattg ttttgggcgg taagcttgcg
    17281 tttgacgacg agatactcca cattcagcga gagtttcccc ttctcgatgg tataacccat
    17341 atacttaccg ccgtaaggac tcatggggct gagatccata tccttgaaag ctgccttgag
    17401 ttcgacatag aggtcttccc tcagcgggtt tatcttgccc gtgatctcca ggggtgcgta
    17461 attatcgtac tttgccttca agtcaacatc ggcaaacttg ttctcttcag aagagagccc
    17521 agaaatccgg ccgccaacct ctgtaagatt cgcagaaaca ttaggtttga catgtctgtc
    17581 gaggaaatct atcgtcccgc cctgcagcgt cactttttcg actgtgaccg tcttatcagg
    17641 agctttctta ttctccggcg ggggtatctt cgttgtcgtg gcattctctc cggcaacagc
    17701 accatttgcc tcctcagcgc ccgagacgtc ctgaacgtta agcgagccat cctcattaat
    17761 gataatgcga gaatagaaat tagccatcgc tacatcgtca atattcaaga agaaggggtt
    17821 gtagccagca cggaggttcc cgaaatggag cgaatcccat ttcaggaagt cttcagcgtt
    17881 ggacttgtcc agcgaccaga acttcgcgag gaatagttcc cccttgtatg acaccttagg
    17941 gctcccgcgt tctccatacc ccagagtgag agccccagac gtcgaaaaat ctccttcagc
    18001 aaggacgatc ttcaccctgt cgctaagata tccctgaaaa ggcacgatgt ctaccttatt
    18061 acagttcaat cgcacgtttg ctgaccccgg gttcaggaca acagagccga cggctgaaac
    18121 agaccccttc ttgccgaact tgagagaaag agaaactttg ccgctcttgt tcttttctgt
    18181 cgaaatacct tcagccgcca gtttgataat ctccgcgctg aaactcaccg gctgagtggg
    18241 aacaaggtct tctcctttga cgccatatcc ctgcgcagag atcttcttca tcgttactgt
    18301 ccacggtctc tcgttctggt tctgctttgc ggcaggcttc ttcgcatctg gggtaggtgg
    18361 cagaagggtt tgtaaattcc atttgccatc ctctccccgc ttcaccatca agaaaccttt
    18421 ctctgtggag aactcgccga caaccacttc ccttttctga agatcgatcg ctgcctcttt
    18481 catagcgatt tcgggtattc tgagaaactc atccttctcg tccctcttct tcaggctcag
    18541 tgatgtaagg tgagccgaaa ggtcggaaag tcgcacagcc tgctcctttt ccgtctgctg
    18601 aaatgcgtat tccgtcgaaa aatcaagaac gccgtccatg atgtcaaaca ggatctgcct
    18661 gcgatagtaa ggagaatatt tctttaacgg tatcttcgcg agttctgctc ttccctgtga
    18721 tgaaagcggg tcaacagtaa actcgccgga aaccttgact ccttcctctg cctccgtccc
    18781 aagagacaat gtgattgctg acttccagtc ccttgcgtta ctgaaacggt caatcttcac
    18841 ctgaatattt tccagtgtgg atcggaaggt ctcctcttgc aataaatcag agaaggtcac
    18901 tgtcccgtta gtcagatcga tttcatcggc gtataaggaa agcaccggtg actcctcttt
    18961 ccttttcagg acgtacccct ttttaggaat cagggactga aggttcaccc tccctgtttt
    19021 atccctgaca acgtggacct caggggactg caacaggacc tttgagagaa gaacccttcg
    19081 ggacatcagt tcccctgaat cgaccgagat gtcaagcaac ggaagatgaa tgagagggct
    19141 ttccttttta tcaacaatcc ggatgtcctt gaactgaaga cttcccgtca aggtaatgga
    19201 tggagccctg tttttgtact gtgtgaaaaa aagaagggtg ttcgcgtcca gacgccctga
    19261 taggaccttg aagtccatct ctgctggcat ataagcaaga taatgggcaa tatcaaagtc
    19321 attcacgcga atctcaactg aagtttcgag ggaatcttta aagggcttgg ttaaaccctg
    19381 aagagaaatc aggtgaccgt taacgttcgc ctcaa
//
LOCUS       DATKOU010000175         4586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3318101, whole genome shotgun sequence.
ACCESSION   DATKOU010000175 DATKOU010000000
VERSION     DATKOU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3318101"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VMU21_08000"
     CDS             complement(<1..791)
                     /locus_tag="VMU21_08000"
                     /inference="COORDINATES: protein motif:HMM:NF036766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUO77506.1"
                     /translation="MANTHKVIFQPAGRRGEIEEGKTILEAAQALGVDLEALCGNKKV
                     CGKCKVRIEEGYSEKDNLESSMSHIFPPLPSEDELKHIKPEDGPGIRLACSSEIRGDL
                     KIFVPERSRAGKQVVRKVAREISIALDPAVKKYTLTFKPPTLHDMTTGDYERVLKALE
                     DDYGLRGITFDFLALRTLQDALRGGNWTATFTIWMDREIIKVEPGHVDVCYGLAVDIG
                     TTTCVGYLCDLSTGKVINTESMMNPQVPYGEDVMSRITYAMTSDTG"
     gene            complement(953..1576)
                     /locus_tag="VMU21_08005"
     CDS             complement(953..1576)
                     /locus_tag="VMU21_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HUO77507.1"
                     /translation="MKREGATLVGVGDISEALSREIAHLNRGIALAVNKGLNRDSVAL
                     LKRLQRLAEAWLKGHGFRSLSIPPDSDRIHDKYIAKLYHLVSHKTTATCAGLGWIGKN
                     GLLINKRYGSKLSWATVLTNAPLVPDAPVSESQCGECNLCVKHCPSGAFTGKQWSRGA
                     PLKKIVHYEKCRTLKKERRLFEEKPNCGLCVTICPFSRNERMRGNVK"
     gene            complement(1612..2298)
                     /locus_tag="VMU21_08010"
     CDS             complement(1612..2298)
                     /locus_tag="VMU21_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HUO77508.1"
                     /translation="MADEARKKEILEKLKNAVIQYDEDACKEGAQEALDAGLDANEAI
                     FDGLVAGMEEVGRLFEAQEYFVPEILMCADALYAGLDILKPHCQQMDLGVKGSVVIGT
                     VEGDVHDIGKNLVKMMFDVAGFTVYDLGRDVPLDQFVDEQLRTDSELVCLSAMMTTSM
                     IGMPKVIDKIKAKNPNCMIMIGGAPTSKELADKWGADGYAEDASNALKEAIKMVSFLK
                     KLRDEKAGKK"
     gene            complement(2298..3323)
                     /locus_tag="VMU21_08015"
     CDS             complement(2298..3323)
                     /locus_tag="VMU21_08015"
                     /inference="COORDINATES: protein motif:HMM:NF004889.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HUO77509.1"
                     /translation="MNSRERVLKLFKGEEIDRVPCFSGMGNVTETGINKLGYKFAGIH
                     LDAKQMADTAASTYKMFGFECGVVPVDLCVEAEALGCVINVYPHAEGILYPTIKEKLI
                     HNEDEMEVKIPADLARRGRVPLLKEAIKHLKADIGNEVAIGSYVLGPFTLAGQIMELN
                     DLLKLSFKKADKVAKLLDVMADAIILVAKEFEKAGADYITVREMGATTDVLSPRVFKS
                     LILPPLKKVISEIKINSVLHICGKTNDIVTSMMEAGPTAISVEQKNDVAETRKKLGKD
                     ALIFGNYDPYNVLVSGDENLVRSTMRKCIDDGVSAVWPGCDIWPTVPPQNMVAMLDEI
                     KKHGGKK"
     gene            complement(3351..4307)
                     /locus_tag="VMU21_08020"
     CDS             complement(3351..4307)
                     /locus_tag="VMU21_08020"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF002142.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HUO77510.1"
                     /translation="MFRFKTEQKVFTFSGIKIGGQPGENPPLMISSMFHNKDKIVQDR
                     KGNFDRQRAKEIIKKQEELSAMTGIPGMVAMVANTPEEAKIYIDFYLETTDMPFGIDM
                     WVAEQRAKATEYVAQLGVQNKFLYNSITPWDKDIKGQVQRLKDLGIKHVVVQAFDDQD
                     QSPAGRLKSMESILAQGADAFDTIIVDTSVMNLPSTSFSLIANRIIKEKHGLPCGGAY
                     SNGTHMWHEIKNQWGLDGFKAMDAVVQGMASALWSDFNFCGPAITAPRVFPAVASAHM
                     LLSTFVYDETGEIYENPNLPIRKYFGDFIAKLQEGGGRKKGK"
BASE COUNT          939 a         1329 c         1072 g         1246 t
ORIGIN      
        1 cccgtgtcgc tcgtcatcgc ataggtaatg cgggacatga cgtcttcgcc gtagggcacc
       61 tgggggttca tcatggactc tgtgttgatc accttaccgg tactgagatc acagaggtag
      121 ccgacgcacg ttgtggttcc tatgtccacg gcgagtccgt aacagacgtc tacatggccg
      181 ggctccacct tgatgatctc cctgtccatc cagatggtga aggtcgctgt ccagtttccc
      241 cctctcaggg cgtcctggag ggtcctcagg gcaaggaaat caaaggtaat gcccctgagg
      301 ccatagtcgt cctccagcgc cttgagcacc cgctcgtaat caccggtggt catatcatgg
      361 agcgttggtg gcttaaaggt aagggtatat ttctttacgg cagggtcgag ggctatcgaa
      421 atctcccttg cgaccttgcg gaccacctgt ttccctgccc gtgaccgttc cgggacaaag
      481 atcttcaggt cgccccgtat ttcgctcgaa caggcaagac gtatccccgg tccgtcttcc
      541 ggttttatat gtttcaattc gtcttctgag ggaagagggg gaaagatatg ggacatactc
      601 gactccaaat tgtctttttc ggagtagccc tcctcaattc tcactttgca ttttccacag
      661 accttcttgt tgccgcagag cgcttcaaga tccacaccga gggcctgggc agcctcgaga
      721 atggttttcc cttcctcaat ctctccccgc ctccctgccg gctgaaaaat cactttgtgt
      781 gtattggcca tgaatccatc ctccttaatt tcttgattta cgtttggcaa cttgaattca
      841 ccagagagta aatagaactg agaagcgaac agagaatacc acacagcctt ttcttattct
      901 ttcctatgga tgttcccttc agtcctctat caccctgttt actgcatgct gcctatttca
      961 cgttgcctct catgcgttcg tttctcgaaa agggacatat cgttacgcag agcccacagt
     1021 tgggcttctc ctcaaagagg cgcctctcct ttttcagtgt cctgcacttt tcataatgaa
     1081 cgatcttctt gagcggtgct ccccgggacc attgctttcc tgtaaaagcc ccggacggac
     1141 aatgcttcac gcagagatta cattccccgc actgcgattc gctcacaggg gcgtcaggca
     1201 cgaggggcgc attggtgaga accgttgccc atgagagctt cgagccgtac cgcttattaa
     1261 tgagaagccc gttcttgccg atccacccca gtcccgcgca tgtcgcggtg gtcttatggc
     1321 ttacgaggtg gtagagtttg gcgatgtact tgtcatgtat cctgtcagag tcgggaggga
     1381 tggacaagct cctgaagcca tgtcccttca gccacgcctc ggccagcctc tggagtctct
     1441 tcagaagcgc aacggagtcc ctgttcagtc ccttgttgac ggcaagggct atccccctgt
     1501 tcagatgggc aatctcccgc gagagcgctt ccgagatgtc gcccacaccc acaagggtcg
     1561 caccttccct tttcaactgc tctttcagtg atgaggcatt caatggtacg cttatttttt
     1621 cccggccttt tcgtccctca atttcttgag gaagctcacc atcttgatcg cctctttgag
     1681 ggcattactc gcgtcttccg catacccgtc agcgccccac ttatccgcaa gctcttttga
     1741 ggtgggagca cccccgatca tgatcatgca gttgggattc tttgccttta tcttgtcgat
     1801 gaccttgggc ataccgatca tggaggtggt catcattgcg gacagacaca caagctcaga
     1861 gtctgttctc aactgctcat ccacaaactg gtccagggga acgtccctgc caaggtcata
     1921 tactgtaaac ccggcaacgt caaacatcat cttcacgagg ttcttgccga tgtcatggac
     1981 atctccctcc actgtcccga tgaccacaga gccctttact ccgaggtcca tctgctgaca
     2041 gtgcggcttg aggatgtcga gtcctgcata aagggcatcg gcacacatca ggatctcggg
     2101 gacgaaatat tcctgtgcct caaaaaggcg acccacctct tccattcccg ccacgaggcc
     2161 gtcgaagatc gcttcattgg catcgaggcc tgcatccagg gcctcctgcg caccctcttt
     2221 acaggcgtcc tcgtcgtact gaatgaccgc gttcttgagt ttttcaagaa tctccttctt
     2281 cctcgcttcg tctgccatta ttttttccct ccgtgttttt ttatttcatc aagcatcgca
     2341 accatgttct ggggggggac agtgggccat atgtcgcacc cgggccatac tgcgctcact
     2401 ccgtcatcga tgcacttcct catggtggac ctcacgagat tctcatcacc cgagacgagg
     2461 acgttgtagg ggtcgtagtt gccgaaaatg agggcatcct tacccagttt ctttctcgtt
     2521 tcagccacat cattcttctg ttcaacactg atggccgtgg ggccagcctc catcatagag
     2581 gtcacgatat cgttagtctt tccgcatatg tgaagcactg aattgatctt tatttcactg
     2641 atgacttttt tgaggggcgg aaggatgagg ctcttgaaga ccctcggact caggacgtcc
     2701 gtggtggccc ccatttccct taccgtgata taatccgcgc cagctttttc gaattccttt
     2761 gcgacgagga ttatagcgtc cgccatcaca tcaaggagtt tcgcaacctt gtcagctttt
     2821 ttgaacgaga gcttcagaag gtcattcaac tccataatct gccctgccaa ggtaaaggga
     2881 ccgaggacat acgagccgat tgccacctca ttgccaatat cggccttgag atgctttatg
     2941 gcctccttga gaagaggcac cctgccccgt ctggcgagat cagcgggtat cttcacttcc
     3001 atctcatcct cattatggat gagcttttcc ttgatcgtcg ggtaaaggat tccctctgcg
     3061 tggggataca cattaatcac gcatcccagg gcctctgctt ccacgcagag gtcaacggga
     3121 accaccccgc actcaaatcc gaacatcttg taggtggaag ccgcggtatc ggccatctgc
     3181 tttgcgtcca gatgaatgcc ggcaaacttg tagccaagtt tgttaatccc tgtctcggtc
     3241 acattcccca ttccgctgaa acaggggacc ctgtcgatct cctcaccctt aaacagcttt
     3301 aacactcttt cccttgaatt cattaacgtg gttcctcctt tctctcttcc ttattttcct
     3361 tttttccttc cgccgccttc ctgcagtttt gcgataaaat ccccgaagta tttccgtatg
     3421 ggaaggttcg ggttttcgta tatctcgccg gtctcatcat acacaaatgt ggagagcagc
     3481 atgtgcgcgc tcgccactgc gggaaatacc cgcggtgccg tgatcgcagg accgcagaaa
     3541 ttaaaatcgc tccagagggc ggacgccatg ccctggacca ccgcatccat ggccttgaat
     3601 ccgtcaagcc cccactgatt cttgatctca tgccacatat gggtcccgtt tgagtatgcg
     3661 ccaccgcacg gcaacccgtg tttctccttg atgatcctgt ttgcgatgag ggagaatgag
     3721 gtggacggca ggttcatgac cgaggtgtcg acgataatgg tatcaaacgc atcagcgccc
     3781 tgggcaagaa ttgattccat ggacttgagc ctccctgctg gggactggtc ctggtcatca
     3841 aatgcctgaa ccaccacatg cttgattccc agatccttca gcctctggac ctgtcccttt
     3901 atgtccttat cccatggggt tatgctgtta tagaggaatt tgttctgcac cccgagctgt
     3961 gccacatact cagtggcctt ggccctctgc tccgcaaccc acatgtcgat tccgaagggc
     4021 atatctgtgg tctcgagata aaaatcgata tagatctttg cctcttccgg ggtgtttgcc
     4081 accatggcga ccatgccggg gatcccggtc attgccgaaa gttcttcctg ctttttgatg
     4141 atctccttag ccctctgtct gtcgaaattt cctttcctgt cctggacaat cttgtccttg
     4201 ttatggaaca tagaagaaat catgaggggc ggattctctc ccggctgtcc cccgatcttt
     4261 attcctgaga aagtaaagac cttctgttca gtcttgaatc taaacattcc ctctccttgt
     4321 cggtcgattt gtgatataat gaaatctctg tatttgtctc gggcagcgat tcccttctcc
     4381 gggatcgctg cccttttctt taagtcttta agacgatctg tgctttcgca cccaaactct
     4441 agaacacccc gtggtcaaat ttcggagcag gcagatactc ccacttctcg cctttcctga
     4501 attttgcctc aatatcaaga cacttgagcg catattcgaa ggcatacgga ataccgggac
     4561 cgccaggcac aaaacctgcc aggatc
//
LOCUS       DATKOU010000176         4340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3704100, whole genome shotgun sequence.
ACCESSION   DATKOU010000176 DATKOU010000000
VERSION     DATKOU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3704100"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..875
                     /locus_tag="VMU21_08025"
     CDS             132..875
                     /locus_tag="VMU21_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HUO77511.1"
                     /translation="MQAKSKPFLIARTGFFIALMAGLVALLSGPGTNYGLWNYRTGIS
                     VLRWAAYGGVAAAALSLIGLIASLRIVIVRGFLWALPGVIMGASLIGLMVHWKQVAES
                     VPRIHDITTDTASPPGFVAILRFRQSDENSPAYGGAEVAARQLKAYPDIIPLILPVPP
                     DQAFERALAVARHLGWQIIDADKTTGRIEAVATTFWFGFRDDIVIRLRQKDSGSRADI
                     RSESRVGVSDLGKNAERIRKFLKAMKEWK"
     gene            916..1500
                     /locus_tag="VMU21_08030"
     CDS             916..1500
                     /locus_tag="VMU21_08030"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HUO77512.1"
                     /translation="MGKSRLEAFSDGVIAIIITIMVLELKVPHGESIDALAPLIPVFL
                     SYVLSFVYVGIYWNNHHHMLHTSNKVTGPILWANLHLLFWLSLFPFATGWMGENHFAA
                     APSAFYGVVLLMAAIAYWILQQLIIASQGPDSLLKKAVGSDWKGKVSPLIYLIAIPTA
                     FWSQWISQGLYVLVALVWLVPDRRIENALTSKER"
     gene            1721..2512
                     /locus_tag="VMU21_08035"
     CDS             1721..2512
                     /locus_tag="VMU21_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO77513.1"
                     /translation="MISTSLYVFLFLVGGLGGFLSGLLGIGGGIVMFPVLLYMPPLLG
                     LEPIAVKAITGLTMAQGFFSAFTATFFYKRERLISKGLVLTLGVSLFVSSLTGSLISK
                     MVPDTPILLIFGFLAILAAIMMLIPRDYSSDDMKAGEVSFNRKLAVMTGLLIGVLLGI
                     VGQGGAFIIIPVLLYVLKIPLRIAFGSTLAIGLLSSGAGLTGKVATGQVPFLMAAALL
                     AGAMPMAYIGGSAGKRTDTKVLRWLLACLILLSAIKIWSDILAKF"
     gene            2546..2776
                     /locus_tag="VMU21_08040"
     CDS             2546..2776
                     /locus_tag="VMU21_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HUO77514.1"
                     /translation="MPKTFTLFLSTSPYSYENTLTAVKTAESALEKGHAVNLIASGDG
                     VYCFLKKQKARGITNAEEEFSRLIQKGLKVYL"
     gene            2914..3267
                     /locus_tag="VMU21_08045"
     CDS             2914..3267
                     /locus_tag="VMU21_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HUO77515.1"
                     /translation="MGKIAMILKRSPYGDINAAEAVRHAMGGAAGELSIDLILVDSGV
                     LLAKKGQDDTGTGFTNLEGALKDCIDMGVEVYADKASVREQHLESADIVDGVKIASAA
                     KIAELTLEAKTTMIF"
     gene            3279..3560
                     /locus_tag="VMU21_08050"
     CDS             3279..3560
                     /locus_tag="VMU21_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrH/TusB family sulfur metabolism protein"
                     /protein_id="HUO77516.1"
                     /translation="MLVIVKSAPDTTDGKRGIKLARDMAADVVLLQSGVYFAQLERLE
                     GFCGIAYVLDDDARLRGLKDSEIEKGIRRLNYGGLIDIMAEADGVVGMF"
     gene            3601..>4340
                     /locus_tag="VMU21_08055"
     CDS             3601..>4340
                     /locus_tag="VMU21_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO77517.1"
                     /translation="MAKIVVLGGSFGGLTAALEIKRLLRNKAEITVVSEDDRFVFNPS
                     LPWLAMGWRKAGDITLPIRPILQKKAITFIHGKAKGVDAEGSRVITEASQIPYDYLVI
                     ATGPHLAFEEVPGLGPEKGHTECIFTLDQAERAHEAWEKFLESPGPLVTGSVQGVSCS
                     GPPYEFAFEVDTELRKRKMRHKVPMVFITSEPYVGHFGIGGLMNSKRIMEDEFAKRDI
                     KVIANTAVEEIVPGEVRLKDGTKLPFRLS"
BASE COUNT         1034 a          993 c         1258 g         1055 t
ORIGIN      
        1 cgcaatcttc gggtataatg agcaaagggg gtatcgctct cttttcaggt aagagtcggg
       61 cccccataaa atgatctgtc ggcgtgttta ggggagattt tggcgacctc gaagggagga
      121 agagagtatc tatgcaagcg aaaagtaaac cattcttaat cgcgaggaca ggttttttca
      181 ttgccctgat ggcaggtctt gtcgccctcc tctctggacc cggcacaaac tatgggctat
      241 ggaactaccg gaccggcatc tcggtgctcc ggtgggcagc ttatggaggg gtcgcagcag
      301 cggccctgtc attgatcggg cttatcgcgt ccctccgcat cgttattgtg agggggttcc
      361 tgtgggccct cccgggggta atcatgggcg cgtcccttat aggtctgatg gtacactgga
      421 aacaggttgc agagagtgtg ccgaggatcc atgacatcac tactgacact gcgagtcccc
      481 ccgggtttgt tgcaatcctc aggtttcgtc agagtgatga gaattctcct gcgtatgggg
      541 gagcggaggt tgccgcccgg cagctgaagg catatccgga catcatccct cttatccttc
      601 ctgttcctcc ggatcaggcc ttcgaaaggg cacttgccgt ggcacggcat ttgggatggc
      661 agattatcga cgctgacaaa accacggggc gcattgaagc tgttgcgacg accttctggt
      721 ttgggttcag ggatgacatt gtaatcaggc tcaggcagaa agattcagga agtcgggccg
      781 atattcggtc tgaatcccgt gtcggagtga gtgacctggg aaagaacgcg gaaaggatac
      841 ggaaatttct caaggcgatg aaggagtgga aatgactctt gcctgcggtt ttcacagacc
      901 aggagcgagg cctgaatggg taaaagcagg ttagaagcat tcagcgatgg cgtgatcgcc
      961 atcatcatca ccatcatggt gttggagctc aaggtgcctc atggcgagag catcgatgcg
     1021 cttgcgccat tgatccctgt attcttgagc tatgtgttga gcttcgtcta cgtcggcatc
     1081 tactggaaca accaccacca tatgctgcat acgagcaaca aggtaaccgg gccgatactg
     1141 tgggccaacc tgcatctgtt gttctggcta tcgctgtttc cctttgcaac gggttggatg
     1201 ggagagaacc acttcgccgc ggcgccatcg gccttctatg gtgtcgttct gctcatggcc
     1261 gctatcgcgt actggatatt gcagcaactc atcattgcat cacaaggtcc tgactccctc
     1321 ttaaaaaagg ctgtcggcag tgattggaaa ggaaaggtat cgcccctcat ttacctcatt
     1381 gccattccaa cggcgttttg gtcgcagtgg atctcccaag gtctctacgt tctggttgcg
     1441 ctggtttggc ttgtgccgga ccgacgcatc gagaacgctc ttaccagcaa agaaagataa
     1501 cccgcgcttt cagggaccta aagctcacct atcggaatat gctatactct tttttatatc
     1561 gatagattat gtctatggta agagtcatga ctgcttcgcc cagatcactc catgaagatg
     1621 accgtcttcc tcattctcaa tgtaatctta ctgcgtggtc ctctcacatt cctgattatc
     1681 cccgggaggg acggtaccgg aacaggaggc cggctctgct ttgatatcaa cttcgttata
     1741 tgtatttctc tttcttgtgg gcgggctggg agggtttctc tcaggactgc tcgggatcgg
     1801 aggaggcatc gtgatgttcc ctgttctgct ctatatgcca ccccttttgg gccttgagcc
     1861 aattgccgta aaggcgataa ccggtcttac catggcgcag gggtttttct ctgcctttac
     1921 tgccacattc ttttataaga gggagaggct tataagcaag ggcctcgtgc tcaccctagg
     1981 ggtttctctt ttcgtctcct ccctcacagg ttccctgatc tcaaagatgg tccctgacac
     2041 gccgatatta ctcatattcg gcttcctcgc aatcctcgca gcaataatga tgcttattcc
     2101 aagggattac agttcagatg acatgaaggc cggggaagtg agtttcaaca ggaagcttgc
     2161 ggtcatgacc gggctcctta taggagtgct tttggggatt gtggggcagg gaggcgcatt
     2221 catcattatt ccggtgctgt tatatgtgct gaagataccg ttgcgcatcg cctttgggag
     2281 cacactcgct atcgggcttt tatcatcagg ggccgggctt actggtaagg ttgccacagg
     2341 tcaggtcccc tttctcatgg ctgctgccct tctcgcagga gctatgccca tggcgtatat
     2401 agggggtagt gcgggaaaac ggaccgatac aaaggtgctc cgctggcttc tcgcatgtct
     2461 gatccttctg agcgcgataa agatatggag cgatattttg gctaagtttt gaggtgtcca
     2521 atctaattat gagaaaggag ggcatatgcc aaaaacgttt accttgtttc tgtccacatc
     2581 gccctattcg tacgaaaaca cactgactgc ggtcaagact gctgaatccg cactcgaaaa
     2641 gggtcacgcg gtgaatctca ttgcgtcggg tgacggagtg tactgcttcc tgaaaaagca
     2701 gaaggcaagg ggaattacca atgcggaaga ggagttctct aggctcatac aaaaagggtt
     2761 gaaggtctat ctctgagggg gctgtttaaa cttccgccgt gcggcgcagg atgactatat
     2821 cgaaggtgca gaggtgggtt cccttaaggg tctttttaag acaatggggg aaacagacat
     2881 ctggctgaac atcggagcat agaggaggag aaaatgggca agattgcgat gatattgaaa
     2941 aggtctccct atggtgacat aaacgcggca gaggcggtca ggcatgccat gggaggtgcg
     3001 gcgggcgaat tgagtattga cctgatcctg gtagatagcg gtgtcctcct tgcgaaaaaa
     3061 gggcaggacg acacgggtac gggcttcacg aatcttgaag gtgctttaaa ggattgtatc
     3121 gatatgggtg tggaggtgta tgcagataag gcatcagtga gggaacaaca cctcgagtct
     3181 gcggatattg tggatggggt gaagatagcg agcgctgcaa agatcgcgga actgacgctg
     3241 gaagctaaaa caacgatgat tttttgaggg aggatcagat gcttgtaata gtgaagagtg
     3301 cgcctgacac aacagatgga aaaaggggca taaaattggc cagagacatg gcagctgacg
     3361 tggtgctcct tcagagcggt gtgtatttcg cgcagcttga gaggcttgaa ggcttctgcg
     3421 gcattgcata tgttctggat gatgacgcaa ggctgagggg cttaaaagac agcgagatcg
     3481 aaaagggtat aagaaggctt aactatgggg gccttattga cattatggcc gaagcagacg
     3541 gagtggtcgg aatgttctaa gcaagaaaca gacaggcata aaaacaaatg gagaggtaac
     3601 atggcaaaga tagtggtgct gggcggttct tttggcgggc ttacggcagc tttagaaata
     3661 aaaaggctgc tccgaaacaa ggcggaaatt actgtggtaa gcgaagatga caggttcgtc
     3721 tttaaccctt ccctgccatg gcttgccatg ggatggagaa aagcgggcga catcacatta
     3781 ccgattagac caattctgca gaagaaggcc atcacattca tccatggaaa ggccaagggt
     3841 gtagatgctg agggttccag agtcatcaca gaggcaagtc agattcccta tgactatctc
     3901 gtcatagcaa ccggccccca tcttgcgttt gaagaagtcc cggggctcgg tcctgaaaaa
     3961 ggacatacag agtgcatctt cacccttgat caggcagaga gggcccatga ggcatgggag
     4021 aagttcctcg aaagccccgg tccgcttgtc acaggctctg tccagggggt cagctgttca
     4081 ggtccaccct atgaatttgc ctttgaggta gacacagagc tcaggaagag aaagatgagg
     4141 cacaaggttc ccatggtctt tatcacttca gagccgtatg tgggtcattt cggcatagga
     4201 ggcctgatga actcaaagag gatcatggag gacgagttcg cgaaacggga cataaaggtc
     4261 attgcgaaca ccgcggtgga agagatcgtg cctggcgagg tgaggctgaa ggacggaacg
     4321 aagctcccct tcaggctttc
//
LOCUS       DATKOU010000177         4827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2162469, whole genome shotgun sequence.
ACCESSION   DATKOU010000177 DATKOU010000000
VERSION     DATKOU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162469"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VMU21_08060"
     CDS             complement(<1..676)
                     /locus_tag="VMU21_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HUO77518.1"
                     /translation="MGVGRKIRDWFEIRTGLDEFARVQFREFRVPKNSNVFHTLGLVT
                     VVAYMIQVITGILLLVYYIPHPDFAFKSVQEIMNTVSFGWLLRQVHVVGSSLMVIVVF
                     LHMVSVFFMGGYRKPREMTWVVGGLMFLITLMFCLSGYLLPWTQLSYWATTVVTSIPT
                     AFPYIGDFISRVLRGGEYVTGITLGRFFALHVSILPPLFLFLMVLHIFFVKRTGLSTP
                     PFGLSSE"
     gene            complement(681..1091)
                     /locus_tag="VMU21_08065"
     CDS             complement(681..1091)
                     /locus_tag="VMU21_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HUO77519.1"
                     /translation="MKRRKFLKVLLGLLGSATVVTFAYPFVKFLAPPEGESKTRKLVV
                     KKSEIPIGEAKGFTFNNTPVIVINRPDKGFIALSKVCTHLGCLVTYERGKKILLCPCH
                     AGVYDIEGNVVSGPPPKPLQKFSVRVEGENVVIG"
     gene            1342..1881
                     /locus_tag="VMU21_08070"
     CDS             1342..1881
                     /locus_tag="VMU21_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HUO77520.1"
                     /translation="MEIYENEDAERAELYKLFSDLFMREPTEETLLQAKEMFQMKSDM
                     SPDEIRRDFARIFLSQDLHPSPYESLYNFSVGEKPRLWGKATEEVKAFYESAGLTLDE
                     EATFIPDHLALELLFMNYLVQNGLAEQQRMFLKEHLFWVNEYCDEVKKHAATPFYKEV
                     AHILKEFMLSEQERYGGEN"
     gene            1882..2121
                     /locus_tag="VMU21_08075"
     CDS             1882..2121
                     /locus_tag="VMU21_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HUO77521.1"
                     /translation="MAEPSKEKPLFKQLLEASTVGIQLVLSTFVGFAMGYFLDKFLGT
                     SPWLTGVFLILGIVAGFRELLRVARRQNGTDKKSN"
     gene            2096..2524
                     /locus_tag="VMU21_08080"
     CDS             2096..2524
                     /locus_tag="VMU21_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77522.1"
                     /translation="MELIKKVTKRSVIVIFPLIFLSFLVDWKDERLKFIGLFGNPGLM
                     AVSILIGGIIGLANLKGLVWGIESLLGTHRANTRMVFLSLLRLFILFAVIIILVVMRL
                     INLLGFLIGMTVVFLVLIKEALDMAKEQGKEGPGDSEGNR"
     gene            2625..3185
                     /locus_tag="VMU21_08085"
     CDS             2625..3185
                     /locus_tag="VMU21_08085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUO77523.1"
                     /translation="MLEEYPRKVRLKDGAVLTLRPMTRDDEHGVSMFFSSLPEEVRRY
                     LRNDVTNRKIIARWMRDLNYEKTLPIIAEYEGRIVASSTLHRQTFGWGRHVGEVRIVI
                     DPAFQGRGLGGLLLDEIYRIGASSGLKKLVARIVTKRDDVIKAFEKSGFSHIGVLKRY
                     VKDMNNNYADIAIMVRELKSEIPAQG"
     gene            3196..4389
                     /locus_tag="VMU21_08090"
     CDS             3196..4389
                     /locus_tag="VMU21_08090"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HUO77524.1"
                     /translation="MRDIVIASGVRTAIGSFGGTLKDVPAVELGGIVIREVLRRAKIS
                     HDKVDLVVMGNVLQAGLGQNPARQAAINGGISQYIPSTTVNVVCGSGMESVITAAQKI
                     KAGDAEVVVAGGMENMSQAPYVMTHSRWGQRMGHTQVLDVMIRDGLWCSFGDTHMGIT
                     AENVAKKFSLSREEQDAYAALSQEKAEKAIKAGRFKDEIVPVVVPQKKGDPIPFDTDE
                     YPKFGTTAEKLGKLKPAFDKKGTVTAGNSSGVNDGAAALLVMSADKAYKLGIKPLARI
                     VSYDVRGCEPELMGIGPIFAVQSAIAKVKAELGKEVDLVELNEAFAAQAIACVKTLQI
                     DPDKVNVNGGAIALGHPIGCSGSRVLVTLMYEMQKRDVKVGLAALCVGGGMGFAMIVE
                     RGWYF"
     gene            4447..>4827
                     /locus_tag="VMU21_08095"
     CDS             4447..>4827
                     /locus_tag="VMU21_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77525.1"
                     /translation="MDKLVEILTKKYPRKVVLDDKSEVTFRPLRKDDEKALSDFFRDL
                     PLKDRACLKEDVSDPKVIESWIYNLDYDNVLPIIATHNGTIAGDATLHFNPIGWTKHQ
                     GEIRLTTDINNRGKGLGTMLAQDII"
BASE COUNT         1265 a         1207 c         1317 g         1038 t
ORIGIN      
        1 gctcagagga caacccaaag ggaggggtgg aaagtcccgt gcgttttaca aagaaaatat
       61 gaaggaccat gaggaacaga aagaggggag gcaggatcga gacatgaagg gcaaaaaatc
      121 ttcccagggt tatccctgtc acatattctc cccccctaag gacccttgag atgaaatcac
      181 cgatataagg gaatgcagtc ggaatggagg tgaccaccgt ggtggcccaa taactcagtt
      241 gggtccacgg gaggagatac ccgctgaggc agaacataag ggttatgagg aacatcagcc
      301 cacccacaac ccaggtcatc tctctcggct tcctgtagcc tcccatgaag aacacagaga
      361 ccatgtgcag aaagaccact atcaccatca gactgctgcc cactacatgc acctgcctca
      421 gaagccatcc gaaagatacg gtattcatga tctcctgaac gctcttaaag gcgaagtccg
      481 gatggggaat ataatagacg aggaggagga taccggtgat gacctgaatc atgtacgcaa
      541 caacggtaac gagcccaagg gtatggaaca cattgctatt tttggggacc ctgaattccc
      601 tgaactgaac tctggcaaac tcgtcgagac cagtccttat ctcaaaccag tccctgatct
      661 tcctgcctac tcccatcttc tcatcctatc accacgttct ctccctcaac cctgacagag
      721 aacttctgca ggggtttggg aggaggtcct gaaaccacat ttccctcaat gtcgtacacc
      781 ccggcatggc aggggcagag caatatcttt ttaccccttt catacgtcac aaggcatccc
      841 agatgggtac acacctttga aagggcgata aatcccttat caggccggtt gatcacgatc
      901 acaggggtat tattaaaggt aaaccctttt gcttcaccta tcggtatttc actcttcttc
      961 accaccagtt tcctggtctt tgactcgccc tcgggaggcg caaggaactt tacgaaggga
     1021 tatgcaaaag tgaccacagt tgcagagccc agaaggccca aaaggacttt caggaatttg
     1081 cgtctcttca tttcacctcg ttacgcagga aagaacacaa ttatttttat ccattgtatg
     1141 tgatatcacc caagttgtca aggcaggttc ctgcccttgc aggggcttca gaggcttctc
     1201 tcccttgcac tgccctttgc ccctctcatg gactcctctg aaaaatgccc ctattctccc
     1261 acgaatgatt gcactcctga atcataccta ctataatgtc tgggacaaag ggaaagcgat
     1321 cggaccatgt tggggtgaag agtggaaatc tatgagaacg aagatgccga acgtgcagag
     1381 ttgtataagc tcttctccga tctcttcatg agagagccca cagaggagac cctcctccag
     1441 gcaaaagaaa tgttccagat gaagtccgat atgtcacccg acgagatacg aagggatttt
     1501 gcccgcatct tcctgagcca ggatttgcat ccctcgccct acgaatccct ctacaacttc
     1561 tcggtgggag aaaagccgag actctggggc aaagcaactg aagaggtaaa ggcattctac
     1621 gaatccgcag gcctcaccct cgatgaagag gcaaccttca tcccggacca cctggcgctg
     1681 gaattgctgt tcatgaatta tctcgtgcag aatggtcttg cggaacagca aaggatgttc
     1741 ttaaaggagc atctcttctg ggtgaatgaa tactgcgacg aagttaagaa acatgctgcc
     1801 actccctttt acaaagaagt cgcacatatc ctcaaggaat ttatgctctc cgagcaggag
     1861 agatacggag gggagaactg agtggctgag ccttcaaagg aaaaaccgct gttcaagcag
     1921 cttttggagg caagtaccgt gggcatccag cttgtcctct ccacctttgt cggctttgcc
     1981 atgggatact tccttgacaa gttcctcggt acctctccat ggctcaccgg tgtattcctc
     2041 atcctgggaa tcgttgcagg gtttcgcgag ctcttgagag tagcgaggag gcagaatgga
     2101 actgataaaa aaagtaacta aaaggagcgt cattgttatc ttccccctta tcttcctctc
     2161 attccttgtg gactggaagg acgaacgtct gaagtttatc ggactctttg ggaatcccgg
     2221 cctcatggcg gtgagcattc ttatcggcgg catcatcggc cttgccaatc tcaagggact
     2281 tgtctggggc atcgaaagcc tgctcgggac ccacagggcg aatacaagga tggtattcct
     2341 gagcctgctc cgcctcttca ttctctttgc cgtcattatc atccttgtgg tgatgaggct
     2401 cattaacctc ctcggatttc tcataggcat gaccgtggtc ttcctcgtac tcataaaaga
     2461 ggccctggat atggcaaagg agcaggggaa ggaaggtccg ggagacagtg aggggaaccg
     2521 ctgaggtgtg gatccttaaa cgtacagtta tctgctggct tgaaaactaa aatattatat
     2581 tgacagagac gggtttcctc gacaatcatt aaaaggagaa caaaatgctt gaagaatatc
     2641 ccaggaaagt cagactgaaa gacggcgctg tccttacgct gaggcccatg acaagggacg
     2701 acgaacacgg ggtctccatg ttcttcagtt ccctgccaga agaggtgagg agatatctga
     2761 gaaacgacgt gaccaacaga aagatcatcg cgcgatggat gcgcgatctc aactatgaaa
     2821 agaccctgcc gatcatcgcg gaatatgaag gaaggattgt ggcaagttcg acgctccaca
     2881 gacagacctt tgggtgggga cgccatgtgg gtgaagtacg gatcgtgatt gatcccgctt
     2941 ttcaggggcg cggcctcggc ggactgcttc tcgatgagat ctaccggatc ggcgcaagca
     3001 gcgggctgaa gaaactggtc gcgagaatcg tcaccaaaag ggatgatgtg atcaaggcct
     3061 ttgaaaaatc aggcttcagc catatcgggg tcctgaagag gtatgtcaag gacatgaata
     3121 acaattatgc agatattgca attatggtaa gagaactgaa gtctgaaatc ccggcacaag
     3181 gatgaaggag gctctatgcg tgatatcgta atcgccagcg gtgtgcggac agccatcggc
     3241 agtttcgggg gtaccctgaa agatgtgcct gcggttgagc tgggagggat tgtcatacga
     3301 gaggttctca gacgcgcgaa aatcagccat gacaaggtcg accttgtggt aatggggaat
     3361 gtgctgcagg caggcctggg ccagaaccct gcacgccagg cagccataaa cggcggaatt
     3421 tcccagtata tcccatcaac gacggtaaac gtggtgtgcg gctcgggtat ggagtcggtg
     3481 atcaccgcgg cccaaaagat caaggccggc gatgcagagg tggtggtggc aggcggcatg
     3541 gagaacatga gccaggcccc ttatgtcatg acccattcga gatggggcca gagaatgggt
     3601 cacacccagg tgctcgatgt gatgatccgc gacggactct ggtgctcttt cggtgatacg
     3661 cacatgggaa ttaccgcaga gaatgtcgcg aagaagttca gcctcagccg cgaagagcag
     3721 gacgcgtacg cggcactaag ccaggaaaag gcggaaaagg cgatcaaggc aggccgcttc
     3781 aaagacgaaa ttgtgccggt agtggtgcca cagaaaaagg gggacccgat accctttgat
     3841 actgacgaat acccgaaatt cggaacaacc gccgaaaaac tggggaagct gaaacctgcc
     3901 tttgacaaga agggtacagt gactgcgggg aactcttcgg gggtgaatga cggcgctgca
     3961 gcgctccttg tcatgtccgc tgataaggca tataaactcg gaataaagcc gctggcaagg
     4021 atcgtctcgt acgatgtgag gggctgtgag cctgaactca tgggaatagg tcctattttt
     4081 gccgtacaga gcgccattgc taaggtaaag gccgaactcg ggaaagaggt tgatctcgtg
     4141 gagctcaacg aggcgttcgc agcccaggcc atcgcctgtg tgaagacgct ccagatcgat
     4201 cccgataagg tgaatgtcaa cggcggcgcc atcgccctgg gacatcctat cgggtgcagc
     4261 gggtcgcggg tactggtgac cctaatgtac gagatgcaga aacgtgatgt gaaggtggga
     4321 ttggcagccc tctgcgtggg cgggggaatg gggttcgcaa tgatcgtcga gcgggggtgg
     4381 tatttctgat gcctgcaccc gtgatcgccc ttcaatgtgg gcacaatgga ttctaaggag
     4441 acggcaatgg acaaacttgt ggaaatactg accaaaaaat accccaggaa ggtcgtcctc
     4501 gatgataaga gcgaagtgac cttccggcct cttcgcaagg atgacgagaa ggccctctcg
     4561 gattttttca gggaccttcc cctcaaggac agggcgtgcc tcaaggagga tgtgtcagac
     4621 ccaaaggtga tagagagctg gatttacaac cttgactacg acaatgtcct tcccatcatc
     4681 gccacccaca acgggaccat cgcaggggac gctaccctgc acttcaaccc cattggctgg
     4741 acaaagcacc agggtgagat ccggttgacc actgatatca acaaccgggg aaagggcctc
     4801 gggaccatgc tcgcccagga catcatc
//
LOCUS       DATKOU010000178        15698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4477733, whole genome shotgun sequence.
ACCESSION   DATKOU010000178 DATKOU010000000
VERSION     DATKOU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 15698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15698
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4477733"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VMU21_08100"
     CDS             complement(<1..454)
                     /locus_tag="VMU21_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HUO77526.1"
                     /translation="MPNVVKDALYAFIPIFVAIDIFAIIPIFVSMTEGMPNEKRLTVE
                     RESVITALAVGLTFIAVGEAIFRILSITENDFKIAGGLILLIFAVLDLMGYTKIREQA
                     GKLGVVPLGVPLIVGPAVLATILVLVDNYGIIPTIISFIANLFIVWFSF"
     gene            complement(456..1988)
                     /locus_tag="VMU21_08105"
     CDS             complement(456..1988)
                     /locus_tag="VMU21_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HUO77527.1"
                     /translation="MNKWIVALTVMLPTLIEIIDTSVVNVALDHIRGSLSAGIDESTW
                     TITSYLVSNAIIIPMTGWLSRLFGRKRYLIFSISMFTLSSLLCGSAWNLQSLVLFRIL
                     QGIGGGALQPISQSILLETFPPKQHGMAMAIFGIGIMFGPIVGPLLGGWITDNWSWHW
                     IFFINIPIGILSILMCIFFIVDPPYMKRMKMKIDYWGLAFLAIGLGCLQIVLDKGQRE
                     DWFSSGFITWLTAASVLSLALFIVVEFFAEHPIVNLKTFKNVTFSTGNLVMFFAFFNL
                     FGSIVLLPIFLQTLMGYTATLAGMVLGPGGVATLIAMPVAGRLVTKMNGKILLSFGII
                     VSAYSTYLMSQFNLFADFNTVIWPRIVLGIGMGFLFIPLITLTMAGVRKEDMGNATAI
                     FNLLRNLGGSFGVAFVTTMLARRTQIQQTNLVAHLTPFDPGYQMAAQRASQVLEYSGL
                     NPGLADQGSLGLIYNKFLKEASMVSFNDTFYILSVLMILVLPLVLIMRRPKAGGPVPG
                     MH"
     gene            complement(2007..2432)
                     /locus_tag="VMU21_08110"
     CDS             complement(2007..2432)
                     /locus_tag="VMU21_08110"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HUO77528.1"
                     /translation="MKMDKDLERIIRYFKAREEVSALYLFGSLAVGRGMRGSDIDLAV
                     LIDERKVKKKNCELLKGDYYRASPAFSLHPVDIVVLNTAPPFLKHRILRTGRILFDRN
                     RRLRARFTAHAIIEYLDFKPIEDMFTGAVAGRFRTKFRL"
     gene            complement(2647..3078)
                     /locus_tag="VMU21_08115"
     CDS             complement(2647..3078)
                     /locus_tag="VMU21_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUO77529.1"
                     /translation="MKDGKVFVDTNILVYAYDLSAGDKHRRAVDLMRDLWRGGGGMTS
                     TQVLQEFFVTVTKKIGKPIDVITAKEIVKDFLKWKTVIVDGELILDAIDIHREHQYSF
                     WDSVIIASAVEGGANTLLSEDLSDKHRIKGIVIKNPFKQGL"
     gene            complement(3065..3310)
                     /locus_tag="VMU21_08120"
     CDS             complement(3065..3310)
                     /locus_tag="VMU21_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77530.1"
                     /translation="MERQNVTLSLPKALLKKAKTLAVMKEMSLSDLLRETLEERVKEE
                     TGYQRAKDRQIALMEKGFDLGTKGKPAASREELHERR"
     gene            complement(3574..4713)
                     /locus_tag="VMU21_08125"
     CDS             complement(3574..4713)
                     /locus_tag="VMU21_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HUO77531.1"
                     /translation="MDEIKQPETAQPSRRNHKRNIMAGIVFAFMAIIGVIAVYFYIQY
                     KSIHITTDDAYVDGNIHTIASRVSGTVIKILVNDNQFVKKGDILLELDPADYEVRVNE
                     ALSGLNAENARIPEIDAKIEAARKQVAEMNAMVQTAKANREVQEANLAQTEIDIRRAE
                     TLYKKDAFSKERYEKATTAHKVALAQVKASAEQVKQAETALETQKALVKQAEASRASQ
                     LSVIKQKKAVLETAQLNYGYTGIYAPTDGYVSKKEVELGNRIKEGQPLMAVVPLSDIY
                     VTANYKETQLEKVKPGQKVEIKIDTYPGKTFKGKVDSIMAGTGAVFSLFPPENATGNY
                     VKVVQRIPVKIVLDSNTDPEHVLRIGMSVEPTVVIDKKESRDREK"
     gene            complement(4736..6091)
                     /locus_tag="VMU21_08130"
     CDS             complement(4736..6091)
                     /locus_tag="VMU21_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUO77532.1"
                     /translation="MKKWLVPKRGYKGLSLRIWAVHLISVISTLTSVFVSLSRAEVLT
                     LPQGLRLAQESSRMIKINQEQEKISEGDTLIAKASLLPSINASANQAFFARQPFAVFG
                     SFTVPTSEKNFPSYSMSIQQLLYDFGGTISRYRSSKAILETKKLDTLRVRNFVSLDFA
                     LVYFDLLESEKLLRVAESEVKALEAHLHDAKNLYENGVITKNDLLQAEVRLSDARQKV
                     LNTTNLRAINASRLNTVLSRSLNTDVEVMDLELVAEGYVVLDRQKAFEVAVQERPEVR
                     ILDETMKALDLEEKSRKAEYYPRFFAGASYDYVDNRYLSPEANASLVLGMSLNLFSGG
                     STRAELIKTEYRRRQLLEQKARVEDEVRLEVEKNLLDLSNARERMNVEKDAVGQAEEN
                     LRINMVRYEEGVGTATDVLDAVTLLTTAETNHYRALYDFRRAEASAFYSMGKDLVEVY
                     Q"
     gene            complement(6193..7038)
                     /locus_tag="VMU21_08135"
     CDS             complement(6193..7038)
                     /locus_tag="VMU21_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HUO77533.1"
                     /translation="MRKVAKQVFLLVSALFILGPQGCPGYEHVFRTGDGKTILFHEMI
                     EDIKKVNLVFVGEFHDRSSHHQAQLEIIKALNRSGVPVAIGLEMFRADSQKELDRWVE
                     GSSGQNEFMKAYQSNWTVPWPLYKDIFLYAREHRIPMVGLNVPDEITRKVSREGFSSL
                     GSGDLRHLPPGISCDIDEGYMNFIQRAYRAHYTEGKSFVHFCEAQMVWDKTMAWNLIE
                     FLRNNPQRTVVVLAGTGHSWKRGIPEQVERLSGPTYRVILPEVPDVINPKAVRSIDAD
                     YILLE"
     gene            complement(7035..7790)
                     /gene="mazG"
                     /locus_tag="VMU21_08140"
     CDS             complement(7035..7790)
                     /gene="mazG"
                     /locus_tag="VMU21_08140"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HUO77534.1"
                     /translation="MEKLVEVMAALRRENGCPWDREQTQQSLKPFLVEETYEVLEAID
                     EGNPEKVKEELGDLLFQIIFHCQLAAEKGEFDIHEVMHKISEKMIGRHPHVFGDARYE
                     TPAEVLRQWEERKREEGKNTESILEGIPRELPSLLRAHRIQARAARVGFDWKRVDDVL
                     KKLDEELGEFRQALAGRNQNEIEDELGDIFFVLVNISRFVGVNPEDALRKTISKFISR
                     FRYIEMKAAESGKSLSEMTLEEMDALWDEAKGK"
     gene            8099..9229
                     /gene="hemW"
                     /locus_tag="VMU21_08145"
     CDS             8099..9229
                     /gene="hemW"
                     /locus_tag="VMU21_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HUO77535.1"
                     /translation="MVNSLYIHIPFCMKKCIYCDFLSVPYDKAVAMDYINAVVKELEF
                     RRESAGVLKTAYIGGGTPTTIPTLPLIRLLRTIRDMVGITPDAELTIEANPGTVTGEM
                     ISALSESGINRLSMGIQSFNDNELKLLGRIHDFSEALKSIAAARHAGITNLSIDLIYG
                     IPGQTLQSWTKTVSTAMKISPEHISAYELTPEKNTPLHELISTGKLEKPDEETILGMY
                     SHAMDRFGEAGYSHYEISNFSKPGFQCRHNLNYWNRGQYLGIGAGAHSFIEDKRIRNT
                     GDILEYMALVSQGNLPEEEVLEISRVDAVKESIFLGLRKKEGLDIREFREHLDIDLMK
                     ISDRLIREGLLISDSTHLALTQKGIVISNTVITELFTLLGLG"
     gene            9456..10301
                     /locus_tag="VMU21_08150"
     CDS             9456..10301
                     /locus_tag="VMU21_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HUO77536.1"
                     /translation="MKIAQKRLGASKGRERRVRLVFTAFLAVFLSLSGTLFLFLEWQE
                     AASGPQVYGYKIVNIYPHDPSAFTQGLIYEGGYLYEGTGLYGKSTLRKVELKTGKVTK
                     LLPLSPGYFGEGLTSWKGDLIQLTWREGKGFVYEKESFRLVREFSYPTEGWGITHDDT
                     HLIMSDGSPSLYFLDPGTFIQVRRMEVRDRGTPLSGLNELEYVKGKIYANIWPSERIA
                     IISPQTGRVEGWIDLTGLSGSMGHSPKIDVLNGIAYDRDKDRLFITGKLWPKLFEIKL
                     IPVKP"
     gene            complement(10520..11683)
                     /locus_tag="VMU21_08155"
     CDS             complement(10520..11683)
                     /locus_tag="VMU21_08155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HUO77537.1"
                     /translation="MEIARLNRTSRILSGHLWVFSNELATSPKDFEPGALVELRDRKD
                     NFLGIGYINPHSLIAVRILTRQRETIGPGFFKKRILDALEYRRRLLKDTDSFRAVYSE
                     GDFLPGLIVDKYNDCLSVQFLTAGMDRIRDMILDVLDDVLSPSVIVLRNDSSARLLEG
                     LEREKRVIRGTLDALPVIREDSLLFEIDPLSGQKTGFFLDQRENRKAFAALVGEGRGL
                     DLFCYSGAWGIHLAEKGCELTGVDESESAITQAQKNADVNGLSERCTFKTADVFEFLK
                     SEVIRGARHDFIVLDPPAFVKGKTKVKEALRAYRAINANAMRVLREGGLLATSSCSYH
                     IEREAFIEMLRAAAKEAGRQVRLVELRSQAKDHPVLLSMPETEYLKCAFLEVR"
     gene            11869..12477
                     /locus_tag="VMU21_08160"
     CDS             11869..12477
                     /locus_tag="VMU21_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUO77538.1"
                     /translation="MKQLVLILSAVQIFLMPLQATAQERNNYFSIKAGTISFTDGLRE
                     ANFRTGFDGEIVYGRYLTPNFAVEVGTGYLHDGVYIKQGFNFSQDIQAIPVILTAKVI
                     YPIQPIELFAGAGYSVYFTKYKGFLIDADATTHLLADDNPPVKKATVFGGQFVAGVNY
                     SFSPAFFFGIEGKYIITDAANFKVFSPSLDGYAITGSFGYRF"
     gene            12656..13000
                     /locus_tag="VMU21_08165"
     CDS             12656..13000
                     /locus_tag="VMU21_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77539.1"
                     /translation="MSPAMADIHSFREKLQTLITKFEKDKTHYLSKGYPEAQVRIDFN
                     NPSEKTIHDKLVSLVNLMLDLHKKKAALPPSAEREKIEREIEITDEKIDEIVYGLYEI
                     TEEEWKAIENSK"
     gene            13089..13304
                     /locus_tag="VMU21_08170"
     CDS             13089..13304
                     /locus_tag="VMU21_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77540.1"
                     /translation="MRANQATAEAFFTAFKALKDKEREAFLEKVISDPALREDFIDVA
                     LIEKAKKVKGKSISAKEYFSKRHKERE"
     gene            13301..13561
                     /locus_tag="VMU21_08175"
     CDS             13301..13561
                     /locus_tag="VMU21_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO77541.1"
                     /translation="MSIYQVKLMPEAQKDLDGFSGKLLLRFEKAIVGLYNNPRPLNSV
                     KLSGSVSKYRVRIGDYRILYEIDDSKKVVKVYRLAHRKEAYR"
     gene            13713..15236
                     /locus_tag="VMU21_08180"
     CDS             13713..15236
                     /locus_tag="VMU21_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUO77542.1"
                     /translation="MLLHHLFVRTAKRHGGKLAFIDCTADKRMTYSKALTASLILTEK
                     FRKYEKGFIGIMIPTSPGCGLSFLAALMSGRTPVMINYSTGAQKNALYAQRKCKFKTI
                     ITSQALLERIDCPVVDGMVFIEDIINGISITDKLRAALTAQLPTPFILRSIHRGSEDS
                     DCVILFTSGSEKDPKAVQLTHRNIASNIRDFSTAIHLSHKDRMLANLPYFHVFGLTVN
                     LLTPLYHGMSIISCANPIEYKTICAIVRDQKPTIMVGTPSFLWGYLRKSEAGDFQSVR
                     LMVCGADKCPDALRAEFLYKHGTTLYEGYGATETSPVISVNTPEHNRPGSVGKLLPGV
                     KVRIENYDTGEDCCPGETGRIMVKGDLVMKGYLNDLEETSMRIRHGWYDTGDMGFLDK
                     EGFLWHAGRLKRFVKIGGEMVSLVRVENILEQFLPENVLCCVVELPDPVKGARIVAVV
                     TQRVDEGNIMKKMGEHLPNIALPRQFVVMDELPKMGSGKIDFRHVTEIVCNALYKKQ"
     gene            15349..>15698
                     /locus_tag="VMU21_08185"
     CDS             15349..>15698
                     /locus_tag="VMU21_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HUO77543.1"
                     /translation="MELHTLRIGELRTLLDRKEVAVKDVVDSVYRRIDAVEDRVRAFV
                     TLTRERALEMASEAQRKIDRNLSMPLLGIPLAIKDNMCTRGIRTTCSSKILSQFVPPY
                     ESTVTSRLAEKGYI"
BASE COUNT         4031 a         4059 c         3663 g         3945 t
ORIGIN      
        1 gaaacgaaaa ccaaacaata aagaggtttg cgatgaatga gattatcgtc ggtatgatac
       61 cgtaattgtc aaccagtacg aggatggtcg cgagcaccgc cggtcccacg ataagcggca
      121 cgccaagcgg aacaacaccc agctttcccg cctgttccct tatcttggtg tatcccataa
      181 ggtccagcac tgcgaagata aggagaatga ggccgcccgc gatcttgaag tcattctcgg
      241 tgatactgag gatcctgaat atggcctcgc ccaccgcaat aaatgtgaga cctaccgcaa
      301 gggcggtgat gacggattca cgttcaacgg taagcctttt ctcgttcggc atcccttcag
      361 tcatggacac gaatatgggg ataatggcaa agatgtcgat ggccacaaat atcgggatga
      421 atgcatagag tgcgtccttc accacattcg gcatatcagt gcatccccgg cacaggcccg
      481 ccggcctttg gcctcctcat gatcaggaca agggggagga ccagtatcat cagaacgctc
      541 aggatgtaga aggtatcgtt aaacgagacc atggaagcct ccttcaggaa cttattataa
      601 atgaggccga ggctcccctg gtcagcaagg ccaggattaa gccctgaata ctcaagaacc
      661 tgggatgccc tctgtgcggc catttggtaa ccgggatcaa agggggtgag atgcgccacc
      721 aggttcgtct gttgtatctg ggtccttcgg gcaagcatgg tcgtaacaaa tgcaacgcca
      781 aagctccccc cgaggtttct caggagatta aagatcgctg tggcattacc catatcctcc
      841 ttccttaccc ccgccatggt gagggtaatg agaggtatga agagaaaccc catgccgata
      901 cccagcacaa ttctgggcca gataaccgta ttgaaatcag cgaagaggtt gaactgtgac
      961 atgagatagg tcgaatacgc tgatacgata atcccgaagg aaagaagaat cttcccattc
     1021 atctttgtta ccagcctccc agcaaccggc attgcgataa gcgtcgcgac acctcctggc
     1081 cccagcacca tccctgccag ggtggcggta taccccatga gggtctggag gaaaatgggg
     1141 aggagaacga tgctgccgaa gaggttgaaa aaggcaaaaa acatgacgag attgcctgtg
     1201 ctgaaggtga cgttcttgaa tgttttcagg ttgacaatgg ggtgctcagc aaaaaactcc
     1261 acaacaataa aaagcgcaag ggacaatacg gatgctgcgg tcagccatgt aatgaagcct
     1321 gaggaaaacc agtcttccct ctggcccttg tccaggacta tctggaggca tccaagccct
     1381 atggcaagaa acgcaagacc ccagtaatcg atcttcatct tcatgcgttt catatacggc
     1441 ggatcaacaa taaaaaatat gcacatcagg atcgaaagga taccgatagg aatattaatg
     1501 aagaatatcc agtgccatga ccagttgtcg gtgatccatc cgcccaggag tgggcccaca
     1561 atagggccga acatgatgcc gatgccgaag atagccatgg ccatgccgtg ctgtttgggc
     1621 gggaatgtct cgagcaaaat ggattgggat atgggctgga gtgcgccgcc cccgatgccc
     1681 tggaggatcc tgaagagtac caggctctgg aggttccagg ctgaaccgca gagcagggaa
     1741 ctcagggtga acatggatat ggagaatata aggtagcgct tcctgccaaa gagcctgctg
     1801 agccagcccg tcatgggtat gataatggca ttggatacaa gatacgaggt gatggtccat
     1861 gtggactcat cgatgccggc agagaggctt ccccggatat ggtcaagggc gacattcacc
     1921 accgaggtgt cgatgatctc gataagggtg gggagcatca cggtaagcgc gacaatccat
     1981 ttgttcatag gtcttataag gtcctgttac agcctgaact tcgtcctgaa acggcctgca
     2041 accgcaccgg tgaacatgtc ctcgatgggc ttgaaatcca gatactcgat aatcgcgtgc
     2101 gcagtgaacc tcgccctcag cctcctgttt ctatcaaaga gtatcctccc tgtcctcaga
     2161 atgcggtgct tgagaaaagg cggagcagtg ttgaggacaa caatgtccac agggtgcaaa
     2221 gaaaatgcgg gtgaagcgcg atagtaatcg cctttcagga gttcgcagtt ctttttcttc
     2281 accttccttt catcaatgag gaccgcgagg tcaatatcgc ttcccctcat cccacggcct
     2341 acggccaggc tgccaaagag gtacaaggca gatacctctt ccctggcctt aaaatagcgt
     2401 ataatcctct ccaaatcctt gtccattttc atgcacaaat tatagcacag cggaaaggac
     2461 cagaacgtct ttgcagaaca gcctccccgt aggtagccgg gagagacgtc cctccgtggg
     2521 ataccccgct tttcgaacgg tgcaaaaaca agaaactctt ttgcaattaa tctttacggg
     2581 atttaagact gcgatacgct ttcccgcggg atcgcttatc tccggagaat ccgccttttc
     2641 cctttgtcac agtccctgtt taaacggatt cttaatgaca attcccttga tcctgtgctt
     2701 atccgaaaga tcctcggaaa gaagagtgtt cgctcctccc tcaacagccg atgcgatgat
     2761 cacagagtcc caaaacgagt attgatgctc ccggtgaata tcgatggcat cgaggatgag
     2821 ttccccgtca acaatcacag ttttccattt caggaaatcc ttcactattt ccttcgccgt
     2881 aatgacatca atgggtttcc ctatcttctt cgtgacagtg acgaagaatt cctgcaacac
     2941 ctgcgtgctc gtcattccgc cgcccccccg ccagaggtcc ctcatcaggt ccacggctct
     3001 ccggtgctta tctcctgcag agaggtcata ggcataaacg agaatattgg tatctacgaa
     3061 taccttaccg tctttcatgg agttcttccc ttgacgcagc aggcttccct tttgtcccca
     3121 ggtcgaagcc cttttccata agcgcaatct gcctatcctt tgccctctga tacccggttt
     3181 cctctttcac cctctcttca agggtctccc gcagcagatc gctcagcgac atctccttca
     3241 tcaccgccaa tgtcttggct ttctttaaaa gggctttggg aagcgacagc gtcacattct
     3301 gcctttccat aacagacctc cttccccacg atcatcacat gattatgtgt atcacatatt
     3361 tgcgtgatat gtcaaatact catacacacc gggtctttta agcgatgcaa ccataaacaa
     3421 ctctggtgaa attgatccac ataggggcta aaatcacaga aaacttttct gatgggttcg
     3481 atgatctcga tgagggtcgg gagcatcacg gtaagcgcaa caatcgattt gttcatttaa
     3541 tatgccccga attgagaatt tataagccaa gatctacttt tccctgtctc tcgattcctt
     3601 cttatctatc acgaccgtag gttcgacaga catgccgatc ctcaagacat gctccggatc
     3661 tgtgttcgaa tcaagcacta tcttcaccgg aatccgttgt acgactttga catagttacc
     3721 cgtcgcattt tcagggggaa aaagagaaaa gaccgctccg gtgcctgcca tgatgctgtc
     3781 cacttttccc ttgaatgttt tgccagggta cgtatcaatt tttatctcaa ccttctgtcc
     3841 cggctttacc ttttcgagct gggtctcctt atagttagcg gttacataaa tatcactgag
     3901 gggaacgact gccatgagcg gctgcccttc ctttatccga ttccccagtt caacctcctt
     3961 tttcgacaca tacccgtctg taggcgcata aattcctgta tacccgtaat tgagttgcgc
     4021 ggtttccagc accgctttct tctgttttat caccgacagc tgagaagccc ttgatgcctc
     4081 tgcctgcttc accaaagcct tctgcgtctc caaagcagtc tctgcctgct tcacctgttc
     4141 tgcagaggcc tttacctgtg caagtgccac cttgtgcgcg gtcgtcgcct tttcatatct
     4201 ctccttggag aacgcatctt ttttataaag agtttcagcc ctccgtatat ctatttcagt
     4261 ctgcgcgaga ttggcttcct gaacttcacg atttgctttt gctgtctgca ccatcgcgtt
     4321 catttccgca acctgttttc ttgctgcctc gatttttgca tcaatttcag ggattcttgc
     4381 attctctgca ttcaaccctg ataaagcctc attcaccctc acctcgtaat cagcagggtc
     4441 taattcgagg agaatatccc cctttttaac aaactggttg tcgttcacaa gaatctttat
     4501 gaccgtgcca gaaactcttg acgcaatggt atgaatgttt ccgtccacat aggcatcgtc
     4561 tgtggtgatg tggatgctct tatactggat atagaagtag accgcaataa ctccaatgat
     4621 tgccatgaac gcaaagacaa ttcccgccat gatatttctt ttatgattcc tccgtgaagg
     4681 ctgtgctgtt tcaggttgtt ttatttcatc catatcatcc tcgttgctgc aaaaattatt
     4741 gatacacctc caccagatct tttcccatgg aatagaatgc ggatgcctct gccctccgga
     4801 agtcgtacaa cgccctgtaa tggttggttt ccgcagtggt gagaagggtc accgcatcaa
     4861 ggacatctgt ggcagtgccc actccctcct cgtatctcac catgtttatc ctgaggttct
     4921 cctccgcctg tcccaccgcg tccttctcaa cattcatcct ctcccgcgca ttgctgaggt
     4981 caaggagatt tttctccacc tccagcctca cctcatcctc gacccttgcc ttttgctcca
     5041 gtaattgcct tcgtctatac tctgttttga tcaattcagc tctggtgctc cccccgctga
     5101 agagattcag gctcatcccc aggacaaggg atgcattcgc ctcaggggac aggtatcggt
     5161 tatccacata atcataagaa gctcctgcaa agaatctcgg atagtattca gccttacggg
     5221 atttctcctc caggtcaagg gccttcatgg tttcgtccag tatcctcacc tccggcctct
     5281 cctgaaccgc gacctcaaat gccttctgcc tgtcaaggac aacatatccc tctgcaacga
     5341 gttccaggtc catcacctcg acatcagtat tcagggaacg ggaaagcacg gtgttgaggc
     5401 gcgaggcatt gatggccctg agattcgttg tgttcaggac cttctgacgc gcgtcagaaa
     5461 gcctcacctc tgcctggagc aggtcgttct tggtaatcac cccgttctca tacaggttct
     5521 ttgcatcatg gagatgggcc tcaagcgctt tcacctcgct ctcagccaca cgcagcagtt
     5581 tttcagattc caggagatcg aaatagacga gagcaaaatc aagggataca aagttcctga
     5641 ccctcagggt atcgagcttc ttggtctcaa gtatcgcttt acttgacctg taccgcgaga
     5701 tagtcccccc aaaatcatat agtaactgct ggatgctcat actgtaagaa ggaaagttct
     5761 tctcgcttgt gggcactgta aaagacccga atactgcaaa gggctgacgc gcgaagaacg
     5821 cctggtttgc agacgcatta atggaaggga gcagggaagc ttttgctatg agcgtatcac
     5881 cttcggatat cttctcctgt tcctgattga tctttatcat tctgctgctt tcctgggcga
     5941 gccttaaacc ctgcgggagg gtcaagacct cagcgcggga cagagacaca aagactgagg
     6001 ttaaggtcga gataaccgag atcaaatgaa ctgcccatat cctgagtgat aagcccttat
     6061 acccacgttt tggaactaac catttcttca tttgattgtc cccttcataa atatccccac
     6121 aaactccctg atggtctttc ccgtgtcgct gaagcggtat ctttttctca acatgagtat
     6181 cccttcaacc gtctattcca gcaaaatata gtcagcgtca atagagcgta cggctttggg
     6241 attgatgacg tccgggactt cagggagtat caccctatag gtcgggcctg acagcctctc
     6301 cacctgttcg ggtatccccc tcttccatga gtggcctgtc cctgcaagta cgacgaccgt
     6361 cctttgaggg ttgttcctca gaaattctat gaggttccat gccatggtct tgtcccacac
     6421 catctgggct tcgcaaaaat ggacaaaaga cttcccttcc gtgtagtggg ccctgtaggc
     6481 cctctgtatg aaattcatat acccctcatc aatatcacaa ctgattccgg gaggcaggtg
     6541 cctcagatcc ccgctaccaa gggatgaaaa tccctcgcgg gataccttcc gggtgatctc
     6601 atcaggtacg ttcaggccta ccataggtat gcggtgctct cttgcataga gaaagatatc
     6661 tttgtataaa ggccagggga ctgtccagtt ggactggtac gccttcatga attcattctg
     6721 tcctgagctt ccctctaccc agcggtccag ttccttctgg ctgtctgccc taaacatctc
     6781 gagccctatg gccacaggaa cacccgagcg gttcaatgcc ttaataattt caagttgtgc
     6841 ctggtgatgg ctgctcctgt catggaactc gcccacaaaa acgaggttca ccttcttgat
     6901 atcctcaatc atctcatgga acaggatggt cttcccgtca ccggtcctga atacatgctc
     6961 atagccgggg cacccctgtg gcccgaggat gaacagagca gagaccagaa ggaagacctg
     7021 tttggcgact tttctcattt ccccttcgcc tcatcccata atgcgtccat ctcttcaagg
     7081 gtcatttcag agagactctt ccctgactcc gcagccttca tttcaatata acggaaacgg
     7141 gagatgaact tgctgatggt ctttctcagg gcatcttccg ggttcacccc cacaaacctc
     7201 gagatattca cgaggacaaa gaaaatgtcc cccagctcat cttctatttc gttctggttc
     7261 ctcccggcaa gggcctgtct aaattccccc aattcttcgt caagtttctt aagcacatca
     7321 tcaacccttt tccagtcaaa tccgacccgt gctgccctgg cctgtatcct gtgtgccctc
     7381 agaagggagg gaagttccct gggaatccct tcaaggatgg attccgtgtt cttcccctct
     7441 tctctctttc tctcctccca ttgccttagc acctctgcag gggtttcata ccgggcgtcc
     7501 ccgaatacgt gggggtgcct cccgatcatc ttttccgaaa tcttatgcat gacctcatgg
     7561 atgtcaaact ctcccttttc cgctgcgagc tggcaatgga aaattatctg gaagagaagg
     7621 tcaccaagct cctcctttac cttctcaggg ttgccctcat caatggcctc caatacctca
     7681 taggtctctt cgaccaaaaa gggtttaagg gactgttgtg tctgctccct gtcccacgga
     7741 cagccgttct cacgcctgag tgctgccatg acttcaacga gcttttccaa ttcacccttt
     7801 gacatcatcg aagtatataa tagagtgaga aaaaaggtaa atgacgcgtg atgatcttga
     7861 agcgggaacg gaggacaggc aggagtgctt agcagaggac agacagtccc ggaattttca
     7921 taatcgacag gacattgaga tggccaatct ttattcagtt aacgcaaacg tatcagttcg
     7981 gatctctcag aaggtttttt cttataaagt gtcgcataaa aattcctcac ctgcctgccc
     8041 tctgcttacc tttgtcaccg ctttaagaac ttccctccct atttctgaga tataagcaat
     8101 ggttaattcc ctctatatcc acattccctt ctgcatgaag aagtgcatct actgcgactt
     8161 tctttctgtg ccttacgata aagccgttgc catggactat atcaatgccg tggtcaaaga
     8221 gctggaattc agaagagaga gcgcgggagt attgaaaacg gcctacatcg ggggaggcac
     8281 ccccaccacg atccccaccc ttccactcat caggcttttg agaaccataa gggatatggt
     8341 tggtatcacc cctgatgcgg aactcaccat tgaggccaat cccggtacag tcaccggaga
     8401 aatgatcagt gccctttctg aatcagggat caacagatta agtatgggca ttcagtcatt
     8461 caatgataat gaattaaagc tcctgggaag gatacacgat ttttctgagg ccctcaaatc
     8521 catagcagca gcacgacacg caggtatcac aaatctgtcc atcgacctca tctacgggat
     8581 accaggccag acgctccaaa gctggaccaa gacggtatcg actgctatga agatttctcc
     8641 tgaacatatc tcggcgtatg aacttactcc ggagaaaaat acccctctcc atgaactcat
     8701 ctcaacggga aaactcgaaa aacctgatga agagaccatt ctcgggatgt actcccatgc
     8761 catggacaga tttggtgaag cggggtatag ccactatgag atatccaatt tttcaaaacc
     8821 cgggttccaa tgcaggcaca atctgaacta ctggaacagg gggcagtacc ttggtatcgg
     8881 tgcaggcgcc cacagtttca tcgaggacaa aaggataagg aataccggtg atatcctgga
     8941 atatatggcg cttgtatcgc agggtaatct gcccgaggaa gaagtgctgg agatttcccg
     9001 cgtggatgcc gtaaaggaat ccatattcct cggtctgaga aaaaaagagg ggctggacat
     9061 cagggaattc agggagcatc ttgacatcga ccttatgaaa atatcggatc gattaatccg
     9121 tgagggactg ctcatttcag acagcacaca tttagctctt acgcagaaag gaattgtcat
     9181 ctccaacaca gtgatcaccg agctgtttac cctgctggga ctgggttagt caggattggt
     9241 gaaactaaag aggctcgcca ggtctggggc aagatacgtg aagttattga cgcagccgga
     9301 tatgaggccg cgtgaattca gcaaagcaca gaccgtaatt aggtgagatt gatagataga
     9361 gaggcacagg gggctattgt atcttgcctt ggagcgggcg agcgtcttat catgaagaaa
     9421 cttatgaatt aagcggatta ggctaaaatt tccttatgaa aatagctcaa aaaagacttg
     9481 gagcgtcaaa aggcagagaa aggagggtga ggcttgtttt caccgcattc cttgctgtat
     9541 ttctttcctt atccggaacg cttttccttt tcctggaatg gcaggaggca gccagcggtc
     9601 ctcaggtcta tggatacaaa atcgtgaaca tctaccccca cgatccctcg gcattcaccc
     9661 agggcctgat ttatgaaggc ggttacctct atgaagggac cggcctttat ggaaaatcaa
     9721 ctttgcgaaa ggttgaattg aagacaggca aggtcacgaa actcctccct ctctctccgg
     9781 gttatttcgg cgaggggctc acctcctgga aaggcgatct gattcagctt acgtggaggg
     9841 agggaaaagg ctttgtgtac gaaaaggaga gtttccgcct cgtcagggaa ttctcttatc
     9901 ccaccgaggg ttgggggatt acccatgacg atacgcacct gatcatgagt gacggaagcc
     9961 cttcgctcta tttcctggat cccggaacat ttatacaggt caggaggatg gaggtccggg
    10021 acagaggcac gcctctgagc ggccttaacg agctggagta tgtgaagggg aagatctatg
    10081 ccaatatctg gccaagcgag cgcatcgcca taatctcacc tcaaaccggc cgcgtggagg
    10141 gatggatcga tctcacaggg ttatcaggtt ctatgggcca ctctccgaag attgatgtac
    10201 tgaatggaat cgcctatgac agggacaaag acaggctttt tatcaccggc aagctctggc
    10261 ccaagctctt cgagattaaa ctaattcctg taaaacctta gctcacttta ttcataagct
    10321 tccttatttc gaaaaaggca tccgtctccc ggcaagacaa tggccctctt cgactgtgcc
    10381 gggatctaca cctaacaaca ctctgttctt atgtattgtc atggtccgct cttgcggaca
    10441 agcacataac cctgagtctt gccattggag ccggacacct ctggcgaatt tatgaattat
    10501 tagcactgga agagacggtt tacctcacct caaggaatgc gcatttaaga tactcggtct
    10561 cgggcatgga aagcaggaca ggatggtcct ttgcctggga cctcagttca acaagcctca
    10621 cctgtctgcc tgcttccttt gctgctgctc tcagcatttc aatgaatgct tctctttcga
    10681 tgtgatatga gcaggacgag gtggcaagga gccctccctc cctgagcacg cgcattgcat
    10741 tggcattgat cgccctatag gcccggagcg cttccttcac cttggtcttc cctttcacaa
    10801 atgcaggcgg gtccaggacg ataaaatcat ggcgcgctcc ccggattact tcacttttca
    10861 gaaattcaaa gacatcggct gtcttaaatg tgcagcgttc tgagagaccg ttcacatcag
    10921 cgttcttctg cgcctgcgta atcgcactct cagactcgtc aacccctgtg agttcgcatc
    10981 ctttttctgc caaatgtatt ccccatgcac cagaatagca aaaaaggtcg agccccctcc
    11041 cctctcccac aagagcagcg aaggccttcc tgttttccct ctggtcaagg aaaaaaccgg
    11101 tcttctgtcc tgagagggga tcgatctcaa agagaaggga atcctcccgt atcacaggca
    11161 gtgcatccag ggtccccctg attactctct tttccctttc aagcccttcg agcaaccggg
    11221 ccgaactgtc attcctcagc acaatgacag aaggggacag gacatcatca aggacatcca
    11281 ggatcatgtc gcgtatcctg tccattcccg cggtgaggaa ctggacagag aggcaatcat
    11341 tgtacttgtc cacaatgaga ccgggaagaa aatctccctc actatagacc gccctgaacg
    11401 agtccgtatc ctttagcaat cttcttctat actccagcgc gtcaagtatc ctctttttaa
    11461 agaaaccggg accgatcgtt tccctctgac gcgtgagaat tcttaccgcg atgagtgagt
    11521 gggggttgat ataccctatg ccgagaaagt tgtcttttct atccctcagc tccactaacg
    11581 cacccggctc aaaatccttt ggactcgtcg caagctcatt agagaagacc cagagatggc
    11641 ctgaaagtat cctcgatgtg cggttcaggc gtgcgatttc catggaataa taatgccgca
    11701 ttgaatgaga aaatacaatt gcatcccaag atgcacgttt cggacaccct tttggggttt
    11761 tgacaatctt aatctcgcac taaaaacgcg cgagacttgc ttcctgacac gcatcataag
    11821 caattttgac aacagtatag atttcatgct taaatgaact ttgaagttat gaaacaactc
    11881 gtgcttattc tcagcgctgt ccagatcttt cttatgcctc tgcaggcaac agcccaggaa
    11941 agaaacaact atttttcgat aaaagcagga accatttcct ttaccgacgg tctcagggag
    12001 gctaactttc gcacaggatt cgacggagag attgtgtacg gtcgttatct cactccgaat
    12061 tttgccgttg aggtcgggac tggttatctt catgacgggg tgtacataaa acagggtttt
    12121 aacttttcac aagacattca ggcaataccc gttatactta cagcgaaggt tatttacccc
    12181 attcagccga tagaattgtt tgcgggtgca gggtactctg tgtatttcac aaagtacaaa
    12241 ggttttctga tagacgccga tgcgaccacg caccttttag cggatgacaa tcctcctgtg
    12301 aaaaaggcca cggttttcgg agggcagttt gtggccggtg taaattattc tttctcgccc
    12361 gcctttttct tcggcattga aggaaaatac attatcacag acgcggctaa tttcaaggtc
    12421 ttcagtccaa gtctggacgg ttacgcaata acaggaagtt tcggctacag gttttaataa
    12481 accaagaatt ataagatcga aggacataaa ttaacatgga aattcattat attggaaacg
    12541 tgatgctacc tgtcaaatat accgttgact cataaagtta tgtagccctc attgcataaa
    12601 caagacgaaa cggttgctgt atcgctgaag gcctgtcctt ttgatataat aagccatgtc
    12661 gccggcaatg gctgatattc actccttcag agaaaaactc cagaccttaa tcacgaagtt
    12721 cgagaaggac aaaacccact acctttcaaa aggctatccc gaggcacagg tgaggataga
    12781 cttcaacaat ccttctgaaa aaaccattca cgacaagctc gtctctctcg tcaacctgat
    12841 gctcgacctg cataaaaaga aagccgccct acccccgtcc gccgagcgtg aaaagatcga
    12901 aagagagata gaaattactg atgaaaagat agatgagatt gtttatgggt tgtatgaaat
    12961 aacggaggag gagtggaagg caattgaaaa tagcaaatga acgtgaccgg aagtcaattt
    13021 atgggatcag acagcaacct tgactttagg ttagaataaa actaaacccg aaacaggagg
    13081 tgattctcat gagagcgaat caggcaacgg cagaggcctt tttcactgca ttcaaagcac
    13141 tcaaggacaa agagagagag gcatttcttg aaaaagtcat aagcgatcct gcattaagag
    13201 aagacttcat tgatgtggca ttgattgaaa aggcaaaaaa ggttaagggc aaatctatat
    13261 cggccaagga atatttttct aagcgtcata aagagcggga gtgagcattt atcaagtcaa
    13321 gctaatgccc gaggctcaaa aggaccttga tggcttctct ggcaaactgc tattgaggtt
    13381 tgaaaaggct attgtaggtt tatataacaa tccccgtccg ctcaattctg taaaactcag
    13441 cggcagtgtt tcaaaatatc gcgtcagaat cggcgattac aggattcttt acgagattga
    13501 tgattcaaag aaggtagtca aagtttaccg acttgcccat cgcaaggagg cttacagata
    13561 gtgttgaccg aatgctctag ctccacaaga tgaaaaactc cctatccccg tcctatgatt
    13621 cccatataga catcgtgagt gcccagttca atctgaggtt ttaatgttat gaacaagctt
    13681 ttgtaggacg caggtacaca gcaaggacca tcatgcttct tcatcacctt ttcgttcgga
    13741 ccgctaaaag gcacgggggc aaactcgcgt ttattgattg cacagccgat aagcgcatga
    13801 cctactcaaa ggcgctcact gcctccctca tcctcacaga aaagttcagg aaatatgaaa
    13861 agggctttat cggtatcatg atccccacgt ccccaggatg cggcctctca ttcctcgctg
    13921 ccctcatgag cggccgaact ccggtcatga tcaactattc cacgggagca caaaaaaatg
    13981 cgctctatgc gcaaagaaag tgtaaattca aaaccatcat cacctcgcag gccctgctcg
    14041 aaaggatcga ttgcccggtg gtagacggaa tggtattcat tgaagacatt atcaacggga
    14101 tttccattac agataaactg agggctgcgc tcacggcaca gctcccgact cccttcatcc
    14161 tcaggagcat tcatagggga agtgaagaca gcgattgtgt catcctcttc accagcggaa
    14221 gcgaaaagga ccccaaagcg gtacagctga cccaccgcaa tattgcatca aatatcaggg
    14281 attttagtac ggccatacat ctttcccata aggaccgtat gctcgcgaac ctgccctact
    14341 ttcatgtctt cggccttacg gtaaacctgc tgacacccct ctatcacggc atgtccataa
    14401 tctcctgtgc aaatcccatt gagtacaaaa ccatctgcgc cattgtccgt gaccagaaac
    14461 ccaccattat ggtggggaca cccagcttct tatggggata tctccgcaaa tcagaagcgg
    14521 gtgactttca gagcgtaaga ctcatggtgt gtggagcgga caaatgccct gatgccctgc
    14581 gggcggaatt tctctacaaa cacgggacta ccctttacga agggtatggg gccaccgaga
    14641 catcacccgt gatctcggta aacaccccgg agcacaacag gccggggagc gtggggaagc
    14701 tgcttcccgg agtgaaggtg cgcatcgaga attacgacac gggcgaggat tgctgccccg
    14761 gagaaacggg caggattatg gtaaagggag acctggtaat gaaggggtac ctgaacgatc
    14821 ttgaagagac atccatgcgc atccgtcacg gctggtacga cacaggtgat atgggattcc
    14881 tcgacaaaga gggcttcctg tggcatgcgg gaaggctgaa gaggtttgtg aagattggcg
    14941 gcgaaatggt ttccctggta agggtcgaaa atatccttga gcaattcctt cctgaaaatg
    15001 ttttatgctg tgtggtggag ctgcctgatc ccgtgaaagg agcaaggatc gtcgcagtag
    15061 tgacacagag agtggacgaa gggaatataa tgaaaaagat gggggagcat cttcccaata
    15121 ttgcgcttcc cagacagttc gtggtgatgg atgaacttcc caagatgggg agcggcaaaa
    15181 tagactttag gcacgtgaca gaaatagtat gtaatgctct gtataagaag caatagcctc
    15241 catgtttctg tttcctcatt gcactgactg cgcgctctcc ttttgcattt tcccgaataa
    15301 gagtgctaaa cttttaggat attacctcag cgcccgaaag gaccataaat ggaactccat
    15361 accctcagaa taggtgaact cagaacgctt cttgaccgta aagaggtcgc ggtgaaggat
    15421 gtggtcgatt ctgtatacag gaggattgat gcggtggagg accgcgtcag ggcttttgtg
    15481 accctcacca gggaaagggc tcttgaaatg gcttccgagg cacagaggaa aatagaccgc
    15541 aacctttcaa tgcccctcct cggaatcccc cttgccatta aagacaacat gtgtaccagg
    15601 ggcataagaa caacctgctc ctcaaagatt ctttcgcagt tcgtcccccc gtatgagagc
    15661 acagttactt cgcgattggc tgaaaaggga tatatctt
//
LOCUS       DATKOU010000179         8274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_245536, whole genome shotgun sequence.
ACCESSION   DATKOU010000179 DATKOU010000000
VERSION     DATKOU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245536"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..2738)
                     /gene="polA"
                     /locus_tag="VMU21_08190"
     CDS             complement(96..2738)
                     /gene="polA"
                     /locus_tag="VMU21_08190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HUO77544.1"
                     /translation="MHLYLIDGNSYVYRAFYAIKGLTNSKGFPTNAIYGFTNMLMKII
                     REKKPDGIAVFFDSPVPTERHRIFEGYKAHRPETPGELVQQLPAIRKVISAFQIKMVE
                     MPGYEADDLLCTMAEIAAKKGAEVFLVTSDKDMLQVVGERIKVYDPIKDKVMDEEYVR
                     ERFGVGPERVPEFMALTGDAVDNIPGIKGIGEKTARELLAGVKDLEELLDHPEAIKKE
                     RLRNLVSENTDLVRMSKKLATIDTAVPIDMDVDEIGMREPSWSELLSLFREFEFGSLM
                     KLIPSAKSERQYESILSMEQCKEIFSSLHGSFALDIEATGKDPLRDRIVGLALCREPG
                     KAYYIPFGHSYEGAPEQLDRREVLRMVSGILENPGIPKIGHNLKYDIGMLRQERVEVQ
                     GILYDTMIASYLINPNKPDHNLEDVSFEYLSYRKKSFQEVVGKKRSFSEVPLEEATSY
                     AAEDAALAMELKGALFERLKEEDLDALYFDIEMPLIHVLIDMEEAGFKVDVAKLNDMS
                     KELSREIESIQKRIHFLAGEEFNINSPKQLSKVLFHSLGLKPGKKTKTGFSTELSVLE
                     DLAESHELPGEILNYRSLSKLKSTYTDVLPDLVNLRTGRIHTSFNQTATATGRLSSSE
                     PNLQNIPVRGEWGRRIRDAFIAEQDNLILSADYSQIELRILAHLSKDQGLIDAFMSGV
                     DIHTRTASALFGVAMDKVTSEMRRVAKTVNFGVVYGISPFGLSAALTITPADAKRYID
                     GYFTGHPGVKAYIEQTLSEAKEKGFVLTMFGRKRKVPEIKSQNAITKAQGERLAINSP
                     IQGTAADAIKIAMIRIRERFRHEKVDAKMILQVHDELVFELPGDELDRTTRIVRETME
                     GAVSLLVPLKVDIGYGKSWAEAHG"
     gene            2853..3995
                     /gene="mnmA"
                     /locus_tag="VMU21_08195"
     CDS             2853..3995
                     /gene="mnmA"
                     /locus_tag="VMU21_08195"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HUO77545.1"
                     /translation="MKVIVGMSGGVDSSVTAYLLRKQGHEVEGVSFVLYEARMKNTFS
                     GCCSMESIRDAGETAARLGVEHTAVDLRDEFMTRVIEPFIDAYARGITPNPCILCNRH
                     IKFPHLLRVADERGAEYIATGHYAITESNRQPAVVGQERVCLKKGIDPKKDQSYVLYV
                     LRAEELKRLILPLGEKRKDEVREIARELKLPAARRPESQEICFIGDRNYRTFMHTLVP
                     EERGGPIIDIESRRVVGSHKGIHTYTIGQRKGLGISSQEPLYVVKIDPASHTLYVGPK
                     EAAKMKEFKVDEINWLIKNAELPKCGSAAETQPVEATFSLPSIRASVKVRSMMRDEPA
                     TLSLMPDGRVHVVYDEPQWAPAPGQSAVFYQGDMVLGGGVISEVAA"
     gene            4036..4479
                     /locus_tag="VMU21_08200"
     CDS             4036..4479
                     /locus_tag="VMU21_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HUO77546.1"
                     /translation="MAGKVVSLNISDKKGVRKRPVPEVVLKTAFGIEGDAHASSAWHR
                     QVSLLALESIEKMRGMGLKVNPGDFAENITTEGIDLLSFPIGTHLTIGGNVEVEVSQI
                     GKECHTRCAIFYQAGDCVMPKEGIFVKVLKGGKIKEGDKIVVNSS"
     gene            4518..5021
                     /locus_tag="VMU21_08205"
     CDS             4518..5021
                     /locus_tag="VMU21_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HUO77547.1"
                     /translation="MNYELLMITVAVLTLSDKGSKGEREDTSGPLIGEMLKGMDARVT
                     YYEVLPDEKEVIKEKLREYSLKADLILTTGGTGLSPRDVTPDATLEVIEREIPGIAEI
                     MRAEGMKKTSRSMLSRAVAGTRGVALIINLPGSPRAVRENLGAILDVIPHAIEKIKGD
                     TKECAAP"
     gene            5032..5754
                     /locus_tag="VMU21_08210"
     CDS             5032..5754
                     /locus_tag="VMU21_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HUO77548.1"
                     /translation="MKDVSFPLAFLAGIMSFLSPCVLPLLPSYVSFITGISFEDLTTG
                     ADRSKIRYLTITNSLTFILGFSTVFVALGASSSAVGKFMFEYQEWIRIGGGILIIVFG
                     LFVAGFLKMDFLMRDKKVHLSGKPAGYVGTFLVGMTFAAGWTPCIGPILGTILLYASS
                     KGSAFYGFKLLAVYSLGLALPFFISSLAINSFLSYSKKLMRYMRVIMIISGLLLILFG
                     ILLLTNRVREFTGFLPDFGIKF"
     gene            5833..6303
                     /locus_tag="VMU21_08215"
     CDS             5833..6303
                     /locus_tag="VMU21_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77549.1"
                     /translation="MKNQELRQGGIFTGLPASLLCTLLLVFMGACGGKKEVKQVSQES
                     RISQEAFSVVEQLRTAYLKRDFTVIADYSTTEGYREIVDSLKYFDSVDLTFTPRWVEM
                     EKAKVYVNVSWQGSWKVGKDTFRERGMAILLFEGTPLKLAKIVRGNPFKYPERP"
     gene            complement(6467..6670)
                     /locus_tag="VMU21_08220"
     CDS             complement(6467..6670)
                     /locus_tag="VMU21_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUO77550.1"
                     /translation="MPIFEYKCNACGEDFEKLVFGSQTVQCPKCSSKDIRKKFSRFGM
                     SGVENQGSSGCSSCSSSSCSSCH"
     gene            complement(6683..7888)
                     /locus_tag="VMU21_08225"
     CDS             complement(6683..7888)
                     /locus_tag="VMU21_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUO77551.1"
                     /translation="MKQDEYSEELSRCVRCGSCKAYCPTYDEGLTEAMGARGRLTLLR
                     GLLTHQLEPSPVLSERIFSCIQCGACEKLCPPALHITEMIYHARKLLRPVDQKRKYLR
                     PFIRFLVKRPLLGFRAARILHPLLHPLLSQRDILPFPLSLPYAPLKDDQQVYKPERKI
                     GRVALFTGCSINFLLPSLGISLINVLVHLGYEVVLPKGEVCCGAPLRSLGMEDEAAAL
                     AKKNLTIFSRLNAEAVLSLCPTCVLSLKHHYPGLVGKGLDNVMDISGFLHNKWDPSQP
                     SPLRNFRKVTYHDPCHLNYSLGIKKEPRELIQRAGAELVEPGEEGCCGFGGTFSLTFR
                     DLSRSLREKRVDAYRKTGSEAIITACPGCMLQLSGGMKDIPVFHLVELLEEAICFRTG
                     IPGIIDKVA"
     gene            7966..>8274
                     /locus_tag="VMU21_08230"
     CDS             7966..>8274
                     /locus_tag="VMU21_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HUO77552.1"
                     /translation="MKVGFVYDEVFLQHETPRGHPECKERLITIVSALRDSPLWNKLV
                     HIKPRKADFVDIEMVHQHRYVEKVRQFGGGYIDPDTYISGGSIDAALCAAGAVMETAK
                     Q"
BASE COUNT         1974 a         2042 c         2170 g         2088 t
ORIGIN      
        1 tgactttcct gcccaaactc tatattcccc tctaatattt cctcacaacg aattttctca
       61 taaaaaacaa aatgtgaacc tggcacatcg tgtcgttacc cgtgggcctc tgcccagctt
      121 tttccatatc cgatgtctac cttcaatggc actaataatg aaaccgcgcc ctccatggtc
      181 tcccgaacaa tacgtgtggt tctgtccagt tcatccccgg gcagttcaaa aacgagctca
      241 tcatgaacct gaaggatcat cttcgcatcc actttttcat ggcggaagcg ttcccggatc
      301 cttatcatcg ctatctttat ggcgtcagca gcagtgccct gaatgggtga gtttattgcc
      361 agtctctctc cctgtgcctt ggttatggcg ttctgactct ttatctcggg caccttcctc
      421 ttcctcccga acatggtgag cacgaagcct ttctcctttg cctcactaag ggtttgctct
      481 atgtaggcct ttactcccgg atgccccgtg aaatagccat ctatgtatct cttcgcatct
      541 gccggggtta tggtcagcgc cgccgacagg ccgaagggcg agattccgta cacaaccccg
      601 aagttcactg tctttgcaac tctcctcatt tctgaggtga ccttatccat agctactcca
      661 aagagggctg aggctgtcct tgtatggatg tccactcctg acatgaatgc atcgatgagc
      721 ccctggtcct tgctgagatg tgcgaggatc ctcagttcaa tctgagagta gtcagcagag
      781 aggatgagat tgtcctgctc agcgatgaag gcatccctta tccgtcttcc ccattcgccc
      841 cgtacaggga tgttctggag gttaggttca ctgctgctca gccttcctgt ggcagtggcg
      901 gtctggttga aggaggtgtg aatgcggccg gtacggagat ttaccagatc aggcaggaca
      961 tcggtatagg tggactttaa tttgctcagg ctccggtaat tcagtatctc cccgggaagt
     1021 tcatgggatt ccgcaaggtc ttccaggacg ctcagttctg tggaaaatcc tgtctttgtc
     1081 tttttccccg gcttgagtcc caggctgtgg aacagtacct tgctcagctg ttttggtgag
     1141 ttgatattaa attcttcacc tgcaaggaaa tgaatccttt tctgaatgga ttcaatctcc
     1201 ctggagagct ccttcgacat atcgttgagt ttagccacat ccaccttaaa gcccgcttcc
     1261 tccatgtcaa tcagtacgtg gatgagaggc atctctatat cgaaatagag tgcatcaagg
     1321 tcttcctcct taagtctctc aaaaagcgct ccctttaatt ccatggccag ggccgcatcc
     1381 tcggctgcgt agcttgttgc ctcctccagg ggaacctctg aaaaagatct cttctttccc
     1441 actacttcct ggaatgattt tttgcggtat gagaggtatt caaaggagac atcttcaagg
     1501 ttgtggtccg gtttgttggg gttgatgagg tatgaggcga tcatggtatc gtataatatg
     1561 ccctgcacct ctaccctctc ttgcctgagc atcccgatgt catatttcag gttgtgcccg
     1621 atcttcggga tacccggatt ttctaatatg ccggaaacca ttctgagaac ttctcttctg
     1681 tccagctgtt caggagcccc ttcatatgaa tgaccaaacg gtatataata tgcctttcct
     1741 ggctccctgc ataaggcaag gccgacgatg cggtccctta aagggtcttt ccctgtggct
     1801 tcaatatcaa gagcgaaact accgtgaagg gacgagaaga tctctttgca ctgttccata
     1861 gaaagtatgc tttcgtactg cctttcgctc tttgcagaag ggatcagttt catgaggctt
     1921 ccaaactcga actcgcggaa aagggaaagc aactcagacc atgaaggttc cctcatgcca
     1981 atttcgtcga catccatgtc tatgggcacc gcagtgtcta tggtcgcaag tttcttgctc
     2041 attctcacga ggtctgtatt ttcgctcaca aggttcctga gcctctcctt cttgatagcc
     2101 tcgggatgat caagaagttc ctcaaggtct ttgacccctg cgaggagctc ccttgctgtt
     2161 ttttcgccaa tgccctttat gcccgggatg ttgtccaccg catctcctgt gagcgccatg
     2221 aactcaggga cccgctcagg ccccaccccg aatctctccc tcacatactc ttcatccatg
     2281 accttgtctt tgatagggtc atagactttt atcctttcac ccaccacctg cagcatatcc
     2341 ttgtcgcttg tgaccaggaa gacttcagcc cctttctttg cggcaatctc tgccatggtg
     2401 cagaggagat cgtcagcctc gtatcccggc atttccacca tttttatctg gaaggcggag
     2461 atgacctttc gtatggccgg aagctgctgt accagttctc cgggggtctc gggcctgtgc
     2521 gccttatatc cctcgaatat cctgtgtctt tcggtgggga ccggggagtc aaagaagacc
     2581 gcaataccgt caggtttctt ctctcttatt attttcatga gcatgttggt gaagccgtag
     2641 atggcattag tgggaaatcc cttggaattg gtgaggccct ttatcgcgta aaaggcgcga
     2701 tacacgtatg aatttccgtc gataaggtaa agatgcatgg gaaattatac cacacagccc
     2761 ttttgcagag gattggcaag gtctcgttca atcctcgcag tggtgaaggc gctgcatgga
     2821 aggcgtttta aaggttctgt tatcatacac tcatgaaggt tattgtgggg atgagcggag
     2881 gggttgattc ttcggtgaca gcgtatctgc tcaggaaaca gggacatgaa gtggaaggag
     2941 tgagctttgt cctgtatgag gcgagaatga aaaatacctt ttcgggatgt tgttccatgg
     3001 agtccattcg cgatgcggga gagacggctg cgcgactggg agtggagcac actgccgtgg
     3061 acctgaggga tgagttcatg acccgtgtta tagagccctt tattgatgcc tacgcacgag
     3121 ggatcactcc caacccgtgc atcctctgca acaggcacat taagtttcct catctcttga
     3181 gagtggctga cgagaggggt gctgaataca ttgccacagg ccattacgcc atcacagaaa
     3241 gcaaccggca gccggcagtc gtcggtcagg agagggtttg tttgaaaaaa ggcatcgatc
     3301 cgaagaaaga tcagtcctat gtgctttacg tgctgagggc cgaagaattg aaaaggctca
     3361 tactgccttt gggtgaaaaa aggaaggacg aggtgcggga gattgcaagg gaattgaaac
     3421 tccctgctgc aaggaggccc gagagccagg agatttgctt tatcggggac aggaactatc
     3481 gcacgtttat gcatacgctt gtccctgaag agcgaggggg tcccattatc gacattgaga
     3541 gcaggagggt ggtgggctcc cataagggta ttcataccta taccattggc cagcgtaaag
     3601 gtctcggcat ctcctctcag gagcccttgt atgtggtgaa gatagaccca gcgagtcata
     3661 ccctttacgt cggtccaaag gaagccgcaa agatgaaaga gtttaaggtt gacgaaatca
     3721 attggctgat aaaaaatgct gaactgccaa agtgcggaag tgcggcagaa acacaacccg
     3781 ttgaagcgac tttttctctt ccgagcatcc gggcttctgt gaaggtccgt tccatgatga
     3841 gggacgaacc agccaccctc tccctcatgc ctgacggaag ggtgcatgtg gtgtatgacg
     3901 agccccagtg ggcgccagcg ccgggccaga gcgcggtctt ttaccaggga gacatggtgc
     3961 tgggaggggg agtgatttcc gaagtggcag cttgattatc ttgttactgc caattgcttt
     4021 gtgttacttt agtgtgtggc aggaaaagtt gtttctctca atatcagcga taagaagggt
     4081 gtgaggaaaa ggccggtgcc tgaggtggtg ctgaagaccg ctttcggcat cgagggcgac
     4141 gcccatgcct cttctgcatg gcacagacag gtgagccttc ttgcccttga gagcatagag
     4201 aagatgcggg gtatgggcct taaggtgaac ccgggagatt ttgcagaaaa cattaccaca
     4261 gagggaatag acctgctatc ctttcccatt ggaacacact tgaccattgg agggaatgtt
     4321 gaggtggagg tgagccagat cgggaaggaa tgtcatacgc gatgtgccat attctatcag
     4381 gcaggggact gcgtcatgcc aaaggaaggg atattcgtca aggtactcaa gggtggaaaa
     4441 ataaaggaag gagacaaaat agtggtcaat agctcatagc tcgtagctga tagtcaaaaa
     4501 actacgaact atgaactatg aactatgaac tactgatgat aacggttgcg gtcctcacat
     4561 tgagcgataa gggctcaaaa ggagagaggg aggatacgag cggtcccctg atcggagaga
     4621 tgcttaaagg catggatgcc cgggtaacgt attacgaagt attgcctgac gagaaagagg
     4681 tgattaagga aaaactgagg gaatactccc tgaaggccga tttgatactc actacagggg
     4741 gtacagggct cagcccgagg gatgtgacac cagatgccac ccttgaggtc attgagaggg
     4801 agattccggg aattgcagag atcatgcgcg cagagggaat gaagaagaca tcgaggtcca
     4861 tgctctccag ggctgtggcc ggaaccaggg gagtggcgct tatcatcaac cttcccggaa
     4921 gtcccagggc ggtgagggag aatctcggtg cgatactgga tgttattccc catgccatag
     4981 aaaagataaa aggagacaca aaagaatgcg cagcgccata gggcataacg tatgaaggat
     5041 gtttcttttc ccctcgcatt cctcgcaggg atcatgtcgt tcctgtcgcc ctgcgtgctt
     5101 cccctcctcc cctcttatgt ctcgttcatc accgggatat catttgaaga cctcaccaca
     5161 ggtgcggaca gaagtaaaat acgctatctc accataacca attccctcac cttcatcctc
     5221 ggcttctcaa cagtgtttgt cgccctgggt gcatcatctt ctgcagtagg gaaattcatg
     5281 ttcgaatacc aggaatggat caggatcggc gggggcattc ttatcattgt cttcggcctc
     5341 tttgtcgcgg gatttctcaa gatggatttt ctcatgaggg acaagaaggt ccatctcagc
     5401 ggaaagcccg ccggatatgt ggggacattt cttgtgggga tgacctttgc tgcaggctgg
     5461 acaccttgca tcggtccgat actggggacc atactcctct acgcgagctc gaaggggtca
     5521 gccttctacg ggttcaaact ccttgcagtc tactccctcg gccttgcact ccccttcttt
     5581 atctcatccc tcgcgatcaa cagtttcctc agttattcca aaaagctcat gcgatatatg
     5641 cgcgtgatta tgatcataag cggtctcctc ctgatactct tcggcatctt gctcctcacc
     5701 aacagggtga gggagttcac gggctttctg cccgactttg gtatcaagtt ctgacgctcc
     5761 cggacacgct tgacaatacc gcgggcaatg cttacagtag gctgaaaaaa acgagtgaaa
     5821 tgaggaggta agatgaagaa tcaggaatta aggcaaggcg gcatttttac gggtctaccg
     5881 gcatctcttc tttgcactct gctcttggta ttcatgggag cctgcggggg aaaaaaggag
     5941 gtaaagcagg tatctcagga atccagaatc tcccaggaag ccttttctgt ggtggagcag
     6001 ctgaggaccg cctatctgaa aagggacttc acggtcattg ctgactacag cacgacagag
     6061 gggtatcggg aaatagtgga ctccctgaaa tactttgact cagtagatct gaccttcacc
     6121 ccgagatggg tcgaaatgga aaaggcgaag gtctatgtaa atgtctcatg gcagggcagc
     6181 tggaaggtag ggaaggatac gttccgggag aggggaatgg ccatcctttt gtttgaaggc
     6241 acgcccctga aactcgccaa gattgtgagg ggaaatccct tcaagtatcc tgaaagaccg
     6301 taacgtgtga atcgtgaact gtgaaaagga acaagtggta tatttattat taagcaggtt
     6361 atcagcaaga atacctatat agcttcacag tctgtcatga caaaaccgaa aaccatcatg
     6421 tctcttacgg tctttatcaa ccattcacgt ttcacctttc acggtatcaa tggcagctgc
     6481 tgcaggaact gctgctgcag gagctgcagc ctgaagagcc ttgattttcc acgccactca
     6541 tgccaaagcg cgaaaacttt ttcctgatgt ctttggaact acatttggga cattgtacgg
     6601 tctggctccc gaagaccagc ttctcaaagt cctcaccaca cgcattgcat ttatattcga
     6661 agatgggcat cgcacctccc cgctaggcaa ctttgtctat tataccaggg atgccggtcc
     6721 tgaaacaaat tgcctcttcc aggagttcca cgaggtgaaa taccggtata tccttcatgc
     6781 cgccactgag ctgcagcatg cagccgggac acgcagtgat gatggcttca gatcctgtct
     6841 tcctgtatgc gtcgaccctc ttttccctga gacttctgga aaggtccctg aaggtgaggc
     6901 tgaaggtccc gccaaacccg cagcaccctt cctctcctgg ttccaccagt tccgcgccgg
     6961 cacgctgtat caactccctg ggctctttct ttattcccag ggaataattc aggtggcacg
     7021 ggtcatggta agtcaccttc ctgaaattcc ggagagggga gggttgagac ggatcccatt
     7081 tgttatgcag aaaaccggat atgtccatta cgttatccag tcccttgcct acaagaccgg
     7141 gataatggtg cttaagtgag aggacacacg tgggacagag actgagaaca gcctctgcat
     7201 tcagcctgct gaatatggtg agattcttct ttgccagggc agcagcctca tcctccatgc
     7261 caagacttct caggggggcg ccgcagcaga cctctccctt ggggagcact acctcatacc
     7321 caagatgcac aaggacattt atcagagaga ttcccaggga gggaaggaga aaatttatgc
     7381 tgcagccggt gaatagtgca acgcggccaa tcttcctttc gggtttgtac acctgttggt
     7441 catcttttag aggagcatag gggagggaaa ggggaaaagg aaggatatcc ctttgagaaa
     7501 gaaggggatg caaaaggggg tggagtatcc tcgcggccct gaaccccagc aggggtctct
     7561 tcaccaggaa acgaataaaa ggcctgagat actttctctt ctggtccacg ggcctcaaga
     7621 gttttctggc atgatatatc atctccgtga tatgcagggc gggagggcaa agcttttcac
     7681 acgcgccgca ctggatgcag ctgaatatcc tctcgctgag gacgggagag ggttccagct
     7741 gatgggtcaa caatcccctc aaaagggtaa gccttcccct ggcgcccatg gcctctgtga
     7801 gaccctcatc gtaagtggga caatatgcct tacaactccc gcatcttacg caccgcgaca
     7861 gctcttcact gtattcatct tgcttcatag tgtattctaa tgtatcgtgg cacagccacg
     7921 caaacaggct ttgactaatg atgaatttcc cgatacaatg atgccatgaa ggtggggttt
     7981 gtctatgacg aggtcttcct gcagcatgag acaccgaggg ggcatcctga atgcaaagag
     8041 agactgatca ctatcgtgag tgcccttagg gattcccctc tctggaataa gctagtccac
     8101 atcaagccga ggaaggcaga tttcgtagat attgagatgg tgcatcagca ccgctatgtg
     8161 gagaaggtga ggcaattcgg tggagggtat atagaccctg acacgtacat ttccgggggg
     8221 agtattgatg cggcattgtg tgcggcagga gcggtcatgg agacagcgaa gcaa
//
LOCUS       DATKOU010000180         7526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1022337, whole genome shotgun sequence.
ACCESSION   DATKOU010000180 DATKOU010000000
VERSION     DATKOU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022337"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VMU21_08235"
     CDS             complement(<1..1279)
                     /locus_tag="VMU21_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUO77553.1"
                     /translation="MKRLSSFKAAWVLSLVFALTIFIGSASAEPWKFGVMSDTQWIGA
                     DDGRSPNSVPVDIIKKLNTEFINKDVKFVIEVGDLCDNGSINGEDTRAAFAQELYNAG
                     VGFFPFRGNHDSSQAAAIEFQRIYPQTQTGVMNATPANVFTVPNPDAAVQPFPIVSGS
                     TFTMGSNFSSPQNYIVGGMSYSNLNGLTYSFDYKNVRFVLLDGQAGKDTNGITPGIDP
                     QQPWITAQLSGRPAGTHAFVMSHKGLITENHVDVLFGSDPSKDPDGQNAFFSSLQSNG
                     VRYYIGGHDHMHNRSIITSPDKTSSVQDILCASDSSKFYTPGYPSNDDKYNVPAFGFT
                     RQTQISQELHTVGYYIFTVDGPQVTVDYYSAVPSNVAPAGCTAGSPNCEYLIPTVPAL
                     NFVKAETFGYSFNGHEYLVAQGEPYTAVNETYAG"
     gene            1741..2400
                     /locus_tag="VMU21_08240"
     CDS             1741..2400
                     /locus_tag="VMU21_08240"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HUO77554.1"
                     /translation="MTELLDISGQFPYLGLFVALILGSVGLPLPEDAILMWCGFLISR
                     NTVEPLPALLVVYSGVILSDLLIFSFGRRYGRLIVGHKLFQRILSPGKLSALELKFNR
                     HGALLIFIGRHIFWLRAKIFLTAGIMKMSPRKFLMADAMSALVFVSVMVTAGNIGTGW
                     LPSLKSFIMQPWCVIASVLVIPIGVFVLVRHLIARRQHSCSVMPLCQKELSCVDQLDG
                     L"
     gene            complement(2424..3692)
                     /locus_tag="VMU21_08245"
     CDS             complement(2424..3692)
                     /locus_tag="VMU21_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUO77555.1"
                     /translation="MKHIFVLDTNVLIHDPKSIFRFEDNDIIIPITVIEELDELKKGD
                     GEIPYSARQALKLIGSLCKNGNGRHGDLSRGVALPNGKGGTLRIATDEHSHLSVKDSP
                     DNRIISVAASTAEKNSTHTVAVVSKDTAVRIKAESFGILSQDYKHDKTSIYKEYGRIL
                     SREDCLKRIRSVRYVQSGPDLIRLQGRDTETRIRNGRSLDNISPKNVEQACAIDALTR
                     PDIEVVALTGTAGTGKTLLALSAALHQTTKKSPLYEQVLVGRPTIPLGGNDIGFLPGD
                     IQEKLHPWMQPIFDNLEIIVGTPKGLIKDNMSVRYRSYQYLIDSGILQIEPLTYIRGR
                     SLPRRYFIIDEAQQLRPIDTKTIITRCGENTKIVFTGDLEQVDHPFLDSYSNGLAYLI
                     SKFINEKNFCYLNLSKTARSSVAEQASRLL"
     gene            complement(3878..5089)
                     /locus_tag="VMU21_08250"
     CDS             complement(3878..5089)
                     /locus_tag="VMU21_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO77556.1"
                     /translation="MEIFWIFAGITVLGLIGYGLQIVAVRSYSGSPPNSGEGETLPRS
                     FPAISVLKPLKGLDDNLFDNLESFCVQDYPEYEIIFSLQDYNDPAYKVARKIKDKYPD
                     RDISIIVKHCDEGLNPKVNNLLSAYRIARYEHVLISDSNVLVDKNYLKEIARPMNNHG
                     VGLVSNLIRGIGGRSIGSVFENLHMNSFVMGSVCFLDKFLKMPCVVGKSMLMRKKDLD
                     AIGGLMAFKDILAEDYMIGKMMHESGKKVLLSHYMIKNVNHYWGIRKFINRHTRWGKL
                     RWKIGGPKYFSELMGNTVFMSSLPIIFWEASKTTVSFALLVCLVKTAGDYYMGRRAGS
                     DLNPFLYFLAPVKDIVIGFIWFVPVLSATVVWRGNRFIIGKDSVLLPCPESGFWSWRY
                     RFVNAIRERIA"
     gene            complement(5101..5790)
                     /locus_tag="VMU21_08255"
     CDS             complement(5101..5790)
                     /locus_tag="VMU21_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUO77557.1"
                     /translation="MLLCDFHIHTKYSDGSVELKRTIDLFGQAGFDAIAITDHVVNGD
                     NSFGKLAHRFRLSVTHNNFRRYLSHIKDESERAWQKYKMLVIPGVEISKNHLSADKSA
                     HMLVLDIKEFIPACWSFEEIFLEAKKQNSLTVACHPHHTSELGRDTLFLWNNREKYAR
                     YIDAWEIANRDDVFNVISLKKYPYIANSDFHRTRHLYSWKTLLNCEKDIGSIKQCIRH
                     NKGVAITLFRN"
     gene            complement(6066..7307)
                     /locus_tag="VMU21_08260"
     CDS             complement(6066..7307)
                     /locus_tag="VMU21_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HUO77558.1"
                     /translation="MPRVFPDNFLWGVATSAFQLEGSPYADWASWDSILSEKPDVTNH
                     YSLYKEDLALLKELGVNAYRFSLEWSRIQPREHVWDERALSHSQEIIDILLNNNIEPM
                     VTLHHFTHPLWFITKYPWHEDSSVEHFSRYLDKVLSELHGVRYWITFNEPYVLLLGGY
                     LEGCMPPGIKDVSLGIKALKNILTCHAMAYERIHKKDPHAMVSVAHNMAALAPWKRWN
                     PFDRVLSKIAKYFYNHSLLDAFLTGRLVVKFPFRNALEIVVPIKDKLDFFGVNYYTRI
                     HIRFNPFKKMGVELRHRDIDGYGLTDMGWEIHPYGLEKVLRYASKLKVPLIITENGIA
                     TRDDQRKIKFMRRHVDALERCLNNGIDVRGYFYWSLIDNYEWLQGLDARFGLYTVDFA
                     TLKRKRTNAAAYYSYLIRSRL"
BASE COUNT         1833 a         1785 c         1828 g         2080 t
ORIGIN      
        1 taccggcata ggtttcattc actgcagtat agggctctcc ctgggctaca aggtattcgt
       61 ggccgttaaa actgtaaccg aaggtctctg ccttgacgaa gttaagggcc ggtactgtgg
      121 ggatcaggta ttcacagtta ggagaaccgg cagtgcagcc ggcgggagct acgttggaag
      181 gcactgcaga ataataatcc accgtgacct gtgggccatc gacagtgaag atatagtagc
      241 cgacggtgtg gagttcctgt gatatctggg tctgacgggt gaagccgaat gccggtacat
      301 tgtatttgtc atcgtttgac gggtaaccag gggtgtagaa cttggagctg tcagatgcgc
      361 aaagtatgtc ctgaacgctg gaagtcttat cagggctggt gatgatcgac cggttatgca
      421 tatggtcgtg gccaccgatg tagtagcgta cgccgttgct ttgcaggctg ctgaagaagg
      481 cattctgccc gtccggatcc ttggagggat ctgagccgaa gaggacgtcg acgtggttct
      541 cggtgatcaa acccttgtgg ctcatgacaa aggcatgcgt gccggctggc cggccggaga
      601 gctgggcggt gatccagggc tgctgggggt cgatgcctgg ggtgatgccg ttggtgtcct
      661 tgccggcctg tccgtcgagc aatacaaagc gtacgttttt gtagtcgaaa gaataggtca
      721 acccattcag gttggaataa gacatgccgc caacaatgta attttgaggg ctgctgaagt
      781 tggaacccat ggtgaaagta gaaccggaga caatggggaa aggctgaacc gcagcatcag
      841 ggttcggcac ggtaaaaacg ttcgccgggg tggcgttcat tacaccggtc tgtgtctgcg
      901 ggtagatgcg ctggaactca attgcggcgg cctgcgacga atcatggttg ccgcggaacg
      961 ggaagaagcc gaccccggca ttataaagct cctgggcgaa tgcggcccgc gtatcctcac
     1021 cgttgatgga accgttgtcg cacaaatcgc ccacctcaat gacgaattta acatctttgt
     1081 tgatgaattc ggtattgagc tttttaatga tatcgaccgg taccgagtta gggctcctgc
     1141 catcgtcagc tccgatccac tgcgtgtctg acatgacgcc gaatttccaa ggctcagccg
     1201 atgctgatcc gataaagatt gtgagtgcga agacgagact caacacccat gctgctttga
     1261 aactggatag tcttttcatt gcaaacactc cttttgatag atttttgaga ttgttgcctg
     1321 tcctaatgct tgttcactcc caattaccgc tacttgaacg ctctgccctt cgcggactaa
     1381 gatacaaggc ggtattccct gaactcattc tccttttccc acctcctttc cctttcgatg
     1441 tcctcaccca ttgcaaggaa atccttgctg gttttggctt cctagaatag tggtgccaga
     1501 gcagggtctc ttttgcgtgt ttgaatgagg taccgagttt ttatccctgt gtctgagaat
     1561 acaagacgcg cattacacga ttatgaaaag cccgtgacat tttcatgaag cagaggtcag
     1621 taatgaaaaa agtgcggcga tcactgcgag gcaattaggc agaaaagcac aaaaacagat
     1681 ttagcagaaa gataacctta ctgtaatatc agtgcaatat ttctatggta taaccctttc
     1741 atgactgagc tgttggacat aagcggacag ttcccctatc tcgggctttt cgtggcgctc
     1801 atcctgggga gtgtaggatt gccccttcca gaagatgcga tcctcatgtg gtgcgggttt
     1861 cttatttcca ggaatacggt cgagcccttg ccggcgctgc tggtggtgta ttcaggggtt
     1921 attctcagtg accttctcat attttccttc ggaagaaggt atggccggtt gattgtcgga
     1981 cacaagctat ttcagcggat actctctccc gggaagcttt ctgctcttga actcaagttt
     2041 aacaggcatg gggctcttct tatattcata ggcaggcata ttttttggct cagggcaaaa
     2101 atattcctga cagcaggaat aatgaagatg tccccccgta agttcctcat ggctgatgcc
     2161 atgtccgcac tggttttcgt ttctgtaatg gtcaccgcgg gcaacatcgg aacaggatgg
     2221 cttccctctc ttaaaagctt tattatgcag ccatggtgtg tcatcgcatc agtactagtc
     2281 atccctatag gtgtctttgt acttgtccgg catctcattg caagacgaca acactcctgc
     2341 tctgtgatgc cactgtgtca gaaggaactg tcatgtgtgg atcaactgga tggtctttaa
     2401 cgctgaattg agcgtttcat tgctcataat aatctggacg cctgttcagc cactgatgag
     2461 cgggcagtct tacttaaatt cagataacaa aaattcttct cattaatgaa cttgctaatc
     2521 aagtaagcca atccattcga ataagagtca agaaacgggt gatccacttg ctccaggtcg
     2581 ccggtaaaaa caatttttgt attctcgccg catcgggtaa tgatggtctt ggtgtcaatt
     2641 ggtcgcaatt gctgtgcctc gtcgataatg aaataccgtc tcggcaggga ccgcccccgt
     2701 atataggtga gaggttcgat ttgcagaatg ccggagtcaa tcagatactg ataactcctg
     2761 tagcgcacag acatattatc cttgatcagc ccttttgggg tccccacgat aatctccaga
     2821 ttatcaaaaa ttggctgcat ccagggatga agcttttcct gtatatcgcc ggggagaaaa
     2881 ccgatgtcat ttccgcccaa ggggatggtg ggtctcccta cgagaacttg ttcgtataag
     2941 ggagatttct tggttgtttg gtgaagtgca gcactcaaag caagaagggt ctttcccgtg
     3001 cctgcagttc ccgtaagagc tacaacttca atatcgggtc tggttaaggc atcgatcgcg
     3061 catgcctgtt ccacattttt gggggagatg ttatccagcg atctgccatt ccttatcctc
     3121 gtctctgtat ccctgccctg caatctaatc aaatcggggc ctgactgcac atatctcact
     3181 gaacgtattc tttttaaaca atcctccctg ctcagaatcc ttccatattc tttatagatt
     3241 gaggtcttat catgtttata gtcctgtgac agaatcccga atgactctgc cttgattctt
     3301 accgcggtat cttttgacac gacagccaca gtatgagtgc tgtttttttc tgcggtgctt
     3361 gccgccaccg atattatcct gttatccggt gaatccttca cggacagatg cgaatgctca
     3421 tccgtcgcta ttctcagggt cccccctttc ccattaggga gggccacacc cctcgacaaa
     3481 tcaccatgcc ttccgttgcc gttcttgcat aatgagccaa taagttttaa ggcctgccgg
     3541 gctgagtagg gtatctcccc atcccctttt tttaactcat cgagttcttc aatgactgtg
     3601 atagggatga tgatgtcatt gtcttcgaat ctaaagatgg atttagggtc atgaatgagc
     3661 acattggtat cgagaacaaa gatatgtttc attcactgtc ctcctttttc atggcagttt
     3721 tttgaaaggg ggcttttata aaagaaaggg atgaaagcaa gatgcttttt ggcaaggctt
     3781 tgagggtaaa gatgttgtca tgcctcttct cagcaggcat cagattcgga tttttcaaat
     3841 acaaaagata aaaaaggccg atcctctctc ctcactgtca ggcgatccgt tccctaatcg
     3901 cattcacaaa acgatatctc cacgaccaga agccgctttc aggacaggga agtaatactg
     3961 aatcttttcc aatgataaac cggttacctc tccatacgac cgtagcgctc aacacgggaa
     4021 caaaccagat aaaccctatc acaatgtctt ttaccggcgc aagaaaatag aggaaagggt
     4081 tcaggtctga gccagcgcgt ctgcccatat aataatcgcc agctgttttt acaaggcaga
     4141 cgaggagtgc aaatgacaca gtggttttgg atgcctccca gaaaattatg ggaagtgaag
     4201 acataaatac cgtattaccc attaattcgc tgaagtattt gggcccgcct attttccatc
     4261 tcaacttccc ccatctggta tgcctgttga taaattttct gattccccaa tagtgattca
     4321 cattctttat catgtagtgc gacaggagca cctttttccc gctttcatgc atcatcttgc
     4381 cgatcatata atcttcagcc agtatgtctt tgaacgccat tagaccgcca atggcgtcga
     4441 ggtccttttt cctcatgagc atggatttgc ccactacgca gggcatttta agaaatttgt
     4501 caaggaagca gacgcttccc atgacaaaag agttcatgtg gaggttttca aatacagagc
     4561 cgatggagcg tccgccaatc cctctgataa gattgctcac aaggcccact ccatggttat
     4621 tcatgggcct cgctatctcc ttcaggtaat ttttatctac cagaacattg ctgtcgctaa
     4681 tgagcacatg ctcatacctt gcgattctat acgcagagag caggttattt acctttgggt
     4741 tcaacccttc gtcacagtgc tttacaatga tggagatatc tctgtcaggg tatttatctt
     4801 ttatcttcct ggcaaccttg taagcagggt cgttatagtc ctgaagggag aaaatgattt
     4861 catattcagg atagtcctgg acacagaagc tttcgaggtt gtcgaacaga ttatcatcca
     4921 gtccttttaa cggcttaagt accgatatgg caggaaaaga tcggggaagg gtctcccctt
     4981 cccctgagtt ggggggggaa ccactatagg aacggaccgc tactatctgt agtccgtatc
     5041 ctataaggcc aagaactgtg atgcctgcaa aaatccagaa tatctccatg cgttttcctc
     5101 ctagtttctg aatagggtta tggcaacccc tttgttatgc cgaatacact gcttgatgga
     5161 accaatgtcc ttctcgcaat tcagcaatgt tttccaggag tagagatgcc gggtcctgtg
     5221 aaaatcgctg tttgcaatgt aagggtattt tttgaggctt atcacattga agacgtcgtc
     5281 cctgtttgca atttcccatg catcgatgta tctggcatac ttctctctat tgttccagag
     5341 gaagagcgtg tccctgccca actccgacgt gtgatggggg tggcatgcca cagtgagtga
     5401 attctgtttc ttcgcttcaa ggaaaatctc ctcaaaactc cagcacgcag ggatgaactc
     5461 cttgatatca aggacaagca tatgggctga cttgtcagca ctcaggtggt tcttgcttat
     5521 ctctacaccg gggattacca gcatcttata tttttgccac gcccgttctg attcatcctt
     5581 gatatgggat aaatagcgtc tgaagttgtt gtgcgttacc gacagtctga agcggtgcgc
     5641 aagcttgccg aaggagttgt cgccgttcac gacgtggtcg gtaatcgcaa tggcatcaaa
     5701 accggcctgg ccgaagaggt caatcgtcct ttttaactcc accgaaccgt cagagtattt
     5761 tgtgtggata tggaaatcac acagaagcat tgcctattac aataatgctt tttagttaca
     5821 ggattattaa gggaggatta catttctgtt aaagctgcac tgatcctcgc gggtatgacc
     5881 ttttgagggg ctcgcccgga ttggggcaag ataccacggg ttattggtgc ggccgggtgt
     5941 gaggccgcct gagttaattc atccgaaggc tgtgattagg tgatccctga cgagaaagga
     6001 acagaagggc cattgtatct tgcctaacgg cgggtgagcc cctaagcaaa acatcattaa
     6061 gtgggctaca accggcttct gatgaggtaa gaataatacg cagccgcgtt ggtacgtttt
     6121 cgtttcaaag tggcgaagtc cactgtatag aggccgaatc gtgcatcaag gccctggagc
     6181 cattcatagt tgtcgatgag ggaccagtaa aaatatcccc gcacatcgat cccgttattg
     6241 aggcatcttt caagggcgtc cacatgcctc ctcataaatt taatcttcct ctgatcgtca
     6301 cgcgtggcaa tgccattttc cgtgataatg agggggactt tgagttttga tgcgtatctc
     6361 aggacctttt cgagcccgta cggatgtatc tcccatccca tgtcggtcag cccgtatccg
     6421 tctatatccc tgtgcctcag ctcgaccccc atcttcttga agggattaaa tcgaatatga
     6481 atcctcgtat agtaattgac gccgaagaaa tccagcttgt ccttaatagg gacgactatt
     6541 tccagggcat ttcgaaaagg aaacttaacg acaagtctgc cggttaagaa cgcatccagg
     6601 agggaatggt tataaaaata cttggcgatt tttgaaagaa ccctgtcaaa agggttccat
     6661 ctcttccatg gtgcaagggc ggccatattg tgtgccacgc tcaccattgc atgagggtcc
     6721 ttcttgtgga tcctctcata cgccattgca tgacacgtga gaatattttt caacgccttt
     6781 atacccaacg agacatcttt tatcccgggc ggcatacagc cctcaaggta tccccccagc
     6841 aggagcacgt agggctcatt aaacgtgatc cagtatctca ccccgtggag ttctgaaagc
     6901 actttatcga gataccgtga gaagtgctcc acagaggaat cctcatgcca cggatacttg
     6961 gtgatgaacc agaggggatg cgtgaaatga tgaagcgtaa ccatcggttc gatgttgttg
     7021 ttaaggagta tgtcgatgat ctcctgagaa tgggataagg ccctctcatc ccagacatgt
     7081 tccctgggtt gtatcctgct ccattccaaa gagaagcgat acgcattcac tcccagctct
     7141 ttaagcaaag ccaggtcttc tttatagagg ctgtaatgat tggtcacgtc aggcttttcg
     7201 ctgagtatcg agtcccagga tgcccagtca gcatagggcg acccttcgag ctgaaaggct
     7261 gatgtggcca caccccagag aaagttgtcg ggaaaaaccc ttggcatact actatgaaat
     7321 accatttcag tgattaaagc aatctgcctt tccaggggta ttcatttctt tgaaaaggtg
     7381 ttgggatctt gtgcaccttc cctgtgagat gaccatcgtg caaatatttt agaagttgtc
     7441 ccgtcctttc gttgaagtag aaaggacccc ggttcatatc ctaagctgca acctcctttc
     7501 tgtcaatgga ttttcgctgt aaggca
//
LOCUS       DATKOU010000181        10252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3325173, whole genome shotgun sequence.
ACCESSION   DATKOU010000181 DATKOU010000000
VERSION     DATKOU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325173"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..427)
                     /locus_tag="VMU21_08265"
     CDS             complement(116..427)
                     /locus_tag="VMU21_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77559.1"
                     /translation="MKKSLMLLVMVFVMVSWIGGQGVAHSQKESALSGKVVETMDSGG
                     YTYVLLDNGGKKTWVAMPQMKVVKGSSMSFQPGAEMENFHSKTLNRTFEKIIFSGGPV
                     K"
     gene            665..844
                     /locus_tag="VMU21_08270"
     CDS             665..844
                     /locus_tag="VMU21_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77560.1"
                     /translation="MKIELEGTLIKMTPENDREKTQLNQLWTIIIGCIDEGKKLVPVG
                     QYIPGVKEVAVFNIE"
     gene            952..1458
                     /locus_tag="VMU21_08275"
     CDS             952..1458
                     /locus_tag="VMU21_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77561.1"
                     /translation="MAGVVGAVWGLAGVSLLLGSAIYRLSPLAIEGFSYHFSWHHWIT
                     LVFVVLFMAYSEGYRAFQKGFSPRVAARARYLRDYPRFLHVLCAPIFCMGYFHAPRKR
                     RITSISVTAGIVILVVLVRLIQQPWRGIIDLGVVVGLAWGLISLLVFSVLAFTSKVFP
                     YSPEVPES"
     gene            complement(1516..1992)
                     /locus_tag="VMU21_08280"
     CDS             complement(1516..1992)
                     /locus_tag="VMU21_08280"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HUO77562.1"
                     /translation="MVEMSVKGRVLGTLLLICSMGLLPPYADPEEERWRLAGKSRTDT
                     LWYIDTESISYPSKDVVSVWVRTVPDTTAAGYVEGTESTDDILKNIQGKYFGDYKYTE
                     GLWELDCARSMFRLLYFSVYSKENKIITSPLTPDADWSYIIQGSVGETIQEAVCKR"
     gene            complement(2042..3010)
                     /locus_tag="VMU21_08285"
     CDS             complement(2042..3010)
                     /locus_tag="VMU21_08285"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HUO77563.1"
                     /translation="MKKHALIWALMCMFPVLSSSGAFPFEGPLQVKNQFPLFLILNAP
                     YLERASYEDSFSATLSYSSVFMVKNSPEWSVNLDMELAELNFRLRKNMSDVVQLGIDI
                     PFLSFNSGFMDNFLSSYHNAFGFSDYGRSSRPSNEFLYEVRKNGLIIIQGEGGSVGIG
                     DIRLSAKKVILNNDPVVSLQATIELPTGNASKGYGSGGVDAGLSLLMDKKISEKIEAY
                     FNLGVVFPGNLKGHETVTLKNSIYGGAAVEAGLWEHISLLGQVVFQSSPFPETGISSI
                     DRISALLSIGGRYTSGKNSFEISLTEDPNTAGAPDFTVTIAFKSHL"
     gene            3233..4540
                     /gene="rsxC"
                     /locus_tag="VMU21_08290"
     CDS             3233..4540
                     /gene="rsxC"
                     /locus_tag="VMU21_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HUO77564.1"
                     /translation="MSPLTFKGGIHPPDKKELAKDASIRPARIPKRVIIPLSQHVGAP
                     CKALVSINQDVKKGQIIGEPGGFVSAPVHSSVSGKVIAIGEFPNAMGRMVPSMVIEND
                     GKEEWTTLRDDPDYPNLLPDELKEKVKAAGIVGLGGAAFPTVVKLSPPKEKPVDTVII
                     NGAECEPYLTADYRLMVENPREIVEGLKILMKVLGVNKGFVGIEDNKPEAIEKMKDAV
                     RNEKGIEVFGLEVKYPQGAEKMLIKATTGREVPPRGLPMDVKVVVQNVGTALAVYEAA
                     RYGKPLIERVVTVTGEGIQKPSNLMVRIGTLISSLIEECGGFKDGVGKVISGGPMMGF
                     AVASLDVPVTKGTSGILVIPEEGVLHAEDFEPCIRCGRCIDICPMGLMPSMLSVFAEK
                     GHYEEAKEYNLFDCFECGSCAFVCPSKRPIVQLVRLAKSLVKP"
     gene            4558..5610
                     /locus_tag="VMU21_08295"
     CDS             4558..5610
                     /locus_tag="VMU21_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HUO77565.1"
                     /translation="MEEFKKEHELIVTVSPHVKSEETVSRIMWSVNLSLFPAFAMGTY
                     FFGIKAILVTALCIITAILSEFFVQKSLKRKVTIHDGSAFLTGLLLGMNLPASLWSFA
                     PFTIHVPIIGSFVAVVITKQLFGGLGYNIFNPALIGRAFLLISFPKVMTIWTEPSAAL
                     FALDAKTTATPLGVLKEEGIGKLVEVFGDKMNLYSQLFVGHRAGSIGETSAIALLVGA
                     AFLLFKRYITWHIPLSFLGTAALLAWVFGGKDPQTGKMLFLAGDPIIHLLSGGMLLGA
                     FFMATDYVTCPSVRKGQILFGIGCGALTMLIRLKGGYPEGVMFAILIMNCFAPIIDRG
                     FKTKVFGTVKTRETAQ"
     gene            5607..6200
                     /locus_tag="VMU21_08300"
     CDS             5607..6200
                     /locus_tag="VMU21_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HUO77566.1"
                     /translation="MTAKDIIKIALNLVVIYVIGGLILAAVYAKTSPIMYRNAEEQKK
                     QALKQLIPDADKIEKLGDWSIHDKHAEYFIARKGDETIGYIVQSYGKGYSSYINTLIA
                     VDKDFKVQKINILSHAETPGLGDEIEADSFKGQFKNKDLDHLKVLKTDTTEYIQAISG
                     ATISSRAVTEDAVKNGVKFLMEKVKEGGSESGAAKHG"
     gene            6178..6807
                     /gene="rsxE"
                     /locus_tag="VMU21_08305"
     CDS             6178..6807
                     /gene="rsxE"
                     /locus_tag="VMU21_08305"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HUO77567.1"
                     /translation="MAQQSTVSYWELIKNGIIAENTIFKLALSLCPSIAVTNNLRNGV
                     LMGVAVLFVQTMVNATISVMRKVIHPRIRLPIFMLVISGWVTIVDMSMAAFAPDAYKQ
                     MGLYIQLIVAFASILARAEMFASKNKIAPSMVDGIGMGLGFLFALTVISFFRELLGKG
                     SLWGIPIVNTKPLLIMILPAGGFFAVGILMALFNWIDTRFLKKGTSGAH"
     gene            6825..7418
                     /locus_tag="VMU21_08310"
     CDS             6825..7418
                     /locus_tag="VMU21_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HUO77568.1"
                     /translation="MSGEFTKLFELFIAASLINNFVFTRFLGLCIFFGVSKKMETAIG
                     MSITFTAVMMISASLSWLIFTYVMVPLDITFLKIIVFIGVVAGFVQASDTIMRKTAPG
                     LYYKLGIYLALISTNCIILAVPLINAGEHYSFIQSIAFGFGSGIGFALALVIMASIRE
                     KLELADVPGPFKGLPIAFIITGLIALAFTGFSGLITL"
     gene            7441..8682
                     /locus_tag="VMU21_08315"
     CDS             7441..8682
                     /locus_tag="VMU21_08315"
                     /inference="COORDINATES: protein motif:HMM:NF005503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HUO77569.1"
                     /translation="MESKGMVDRLIRREYASGDYREKRGSFSDTGFFALLLLCVLAFS
                     LSVAYPVYAGVGGGVEAKEHVELLPLLKFTAIFLAGVGLVFGLGLAFAAKRFSVKIDP
                     RVEQVKDNLAHAHCGACGYAGCEQYAEAVVNNPDVPPNLCTPAGARGAEAVALITGKK
                     AEAREPIFARIMCQGGWPKAIKKYKYEGVQDCRAAVLAGGGDKACAYGCLGYGTCARV
                     CPFNAITMNDDHIPVVDITKCTGCRKCEQACPKKVIEVLPASRQVLVACHSRDRGVDT
                     KKNCQVGCIACGKCVKVCPFDAPSISSNLSRIDLDKCEVCGLCVSPCPTNAIRDFIPK
                     RPKAVVLDNCIGCQICMKVCPVNAASGELKKKHAIDQDKCIGCGICTAKCPVQAIDGT
                     FNAHEVFQAAAAKKAAKTTAA"
     gene            8839..9039
                     /gene="rpmE"
                     /locus_tag="VMU21_08320"
     CDS             8839..9039
                     /gene="rpmE"
                     /locus_tag="VMU21_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HUO77570.1"
                     /translation="MKEGIHPDYKEAKVVCACGETFTTQSTKPVIHVDICSKCHPFFT
                     GKQKLMDAEGRVQKFKRKYAKK"
     gene            9118..10005
                     /locus_tag="VMU21_08325"
     CDS             9118..10005
                     /locus_tag="VMU21_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HUO77571.1"
                     /translation="MKNIGGQAVIEGVMMKSPRGWTVAVRDQKGDIHIKREFLRKLPG
                     FLHLPFIRGCVGLYHALAIGMKAIEFSASKAYSTEEEKPMSKTAIASTMGVALLIGIG
                     LFLFLPLYLTKLAGHVVGTITTSSLVFNLTDGVIRVFIFLFYVSVIGLWKDMRRIFEY
                     HGAEHKVIHAYEAGKQLDVENVRIFSPHHPRCGTSFLLIVMVMSILIFSFIPQSWPFG
                     GKFLSRLVLIPVIAGTSYELLRVSAKMKENTLIHLMILPGLMLQRLTTREPDDSQIEV
                     AIKAVNEVLQLEVNNAPEA"
     gene            9989..>10252
                     /locus_tag="VMU21_08330"
     CDS             9989..>10252
                     /locus_tag="VMU21_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HUO77572.1"
                     /translation="MLRKLETIQDKYDELTRLLMSPEILSSPQQYQKYSKEQSDLHPL
                     VEKIGQYRKLLTDIEGADELLRGGDGDMKELAQAELDELRVKRP"
BASE COUNT         2596 a         2329 c         2710 g         2617 t
ORIGIN      
        1 catgtaccaa aaataagaaa gagtatccag agaaaatgga tgaaaacgac accgtttgca
       61 aaaattccgt aaggcatcca ccccggaatg gcataagaag cttatacagg gggtattatt
      121 ttaccggccc ccctgagaag ataattttct caaaggtgcg gttaagggtt ttactgtgaa
      181 aattttccat ctctgcccct ggctgaaaag acatgctcga tcctttcact actttcattt
      241 ggggcatcgc gacccatgtc tttttgcccc cattatcaag caaaacatag gtgtaacccc
      301 cgctatccat ggtttcaacc actttaccgg acagtgcaga ttctttttgg gagtgtgcga
      361 ccccttgtcc gcctatccat gataccatta caaacaccat caccagcaac attaaactct
      421 ttttcatgtt cttaattcct cctgggactg ttggcaattg cagccgtgcg tcctcactta
      481 ggttaacact aatgatttca aaaaatacag tacctcttag acgatggaac agggaaactg
      541 aacaagtggc gtttttcagg agggaccatt ctggaaatgg tcatcttaaa gctctaccga
      601 aaaaaaacaa ttttatatat aattgatacg gaagcattat ccatttctat tcttggaggt
      661 ttatatgaag atcgaacttg aaggcaccct cattaagatg acgccggaaa atgacaggga
      721 gaagacgcaa ctgaaccagc tctggaccat catcatcggc tgcatagatg agggaaagaa
      781 acttgtcccc gtgggccagt acatccctgg tgttaaggaa gtggcagtat tcaatatcga
      841 atagataccg tcgtggtcgt gtagtcatat ctgattgtga aggacaggtt gcgggaccga
      901 gacacggtct tgaagggctt ggtttcggat ccctaaaggg ttgggagcca tatggcaggg
      961 gtagtgggag cagtatgggg attagcaggg gtgtcgctcc tcctcggcag cgcaatctac
     1021 cggttgtcac ccctggccat cgagggcttt tcgtaccatt tttcgtggca ccactggata
     1081 acgttggtat tcgtcgttct gtttatggcc tattccgagg gctaccgtgc gttccagaag
     1141 ggcttttccc cccgtgttgc agcgcgggca agatacctca gggactatcc acgtttcctt
     1201 catgtcctgt gtgcgccaat tttttgcatg ggatatttcc atgctccaag gaagagaaga
     1261 atcacctcca tttcggtaac cgcaggaatc gtcattctcg tcgtcctggt ccgtctcatt
     1321 cagcagccat ggcggggaat aattgatctg ggcgtggtgg tcggtctggc atggggactc
     1381 atttccctgc tcgtcttcag tgttctcgca tttacctcaa aggtgtttcc ctattctccg
     1441 gaagttcccg aatcataaac ggatagcgga caaggtgcac catgccgatc atatggtgat
     1501 ccttatgacg tgagattatc tcttacacac cgcttcctga atggtctctc cgacgcttcc
     1561 ctggattata tatgaccagt ctgcatcagg ggtaagaggg gacgtgatga tcttgttttc
     1621 cttactgtag acagagaagt ataaaagcct gaacatgctc cttgcgcagt ctaattccca
     1681 taacccttca gtatatttgt aatccccaaa atatttcccc tggatatttt ttagtatatc
     1741 atcagtgctc tccgtgccct cgacataccc agcggctgta gtatcgggaa cggttctcac
     1801 ccatacgctc accacgtcct ttgacggata ggatatggac tcagtatcga tataccagag
     1861 ggtgtctgtt ctgctcttgc cagccagcct ccatctctct tcctcagggt ccgcataggg
     1921 agggaggagt cccattgagc agatcagcaa taaggtgccc agaaccctac ccttcacgga
     1981 catttccacc atatgaacat tataactgaa tgaaagctct ccagttcgcc tatggaggtt
     2041 tttagaggtg gcttttgaac gcaatggtta ctgtaaagtc aggtgcaccc gcagtattgg
     2101 gatcctcggt aagggagatt tcgaagctgt ttttccctga ggtatatctt cccccgatgg
     2161 agagcaaggc agagattcgg tctatggacg agatgcctgt ttcggggaag ggggaacttt
     2221 ggaacactac ctgtccgagg aggcttatat gttcccagag tccagcttcg accgccgctc
     2281 ccccgtaaat agagttcttg agcgtgaccg tttcatgccc tttcagattg ccgggaaaaa
     2341 caacaccaag gttaaagtat gcctcgatct tttcagatat tttcttgtcc atgaggagtg
     2401 agagtccggc atcaacgcca ccgctgccgt aacctttgga ggcattgcct gtgggaagct
     2461 ctatagtcgc ctgcaggctc accacaggat cattattcag gatcactttt tttgcgctga
     2521 gacgtatatc cccgattccg acacttccac cctcgccttg tataattatg agcccgtttt
     2581 tgcgcacctc gtagagaaat tcgttgcttg gtctactgct tcgtccgtaa tcagaaaatc
     2641 caaaggcgtt atggtagctg ctgagaaaat tatccatgaa accgctgttg aagctcagga
     2701 agggaatatc gattcccagc tgaacaacgt cgctcatatt ctttctcaat ctgaagttga
     2761 gttccgccaa ttccatatca agattcaccg accattcagg agagtttttc accatgaaca
     2821 cgctcgaata ggaaagggtg gcagagaatg aatcttcgta agacgcgcgt tccagatacg
     2881 gtgcgtttag aataaggaaa agagggaatt gattttttac ctgcaacggt ccttcaaagg
     2941 gaaacgcacc agatgaggag aggactggga acatgcacat gagtgcccat atcaacgcat
     3001 gtttcttcat ttggatatac catagcgagg gatatgtcag gatgtcaata aaagggcgcc
     3061 tttcaatgcg ggtgggcaat cctatctggt caatcgttgg tgtaataagc cattaaaatt
     3121 tcttatagac ctacatgaac cttgacatcg cttaagttac cgtgctatta attaagggct
     3181 ttttggtctt tcgtcatcct gagcaattta tttcgaacag gaggaaccct tcatgagccc
     3241 gctcacattc aagggtggaa tacatccgcc ggacaagaag gaattggcga aagacgcctc
     3301 catcagacca gcacggattc ccaaaagagt gataattcct cttagtcagc atgtgggtgc
     3361 tccctgcaag gctctggtct ccataaacca ggatgtgaag aaagggcaga tcataggaga
     3421 gccgggaggg tttgtttcgg cgcctgtcca ttcatccgtc tctggcaagg taatcgctat
     3481 cggagagttt cccaatgcca tgggaagaat ggtcccttca atggtaattg agaatgacgg
     3541 gaaagaggaa tggaccacgc tcagggacga ccccgattat cccaatctcc tccctgatga
     3601 acttaaggag aaggtcaagg ctgcaggaat tgtgggtctc ggcggcgcag cattccccac
     3661 ggtagtgaaa ctttcaccac ccaaggagaa gcccgtcgac accgtgatca ttaacggagc
     3721 tgaatgcgaa ccctatctca ccgcagatta ccggcttatg gtggagaacc cccgggaaat
     3781 agtcgagggc ctgaagatat taatgaaggt cctgggagtg aataaggggt ttgtgggtat
     3841 tgaggacaac aaaccagaag ccattgaaaa gatgaaagat gcggtaagga atgagaaggg
     3901 catcgaagta tttgggcttg aggtgaaata cccacagggt gctgagaaga tgctcataaa
     3961 ggctactacg ggaagggaag tgccgccaag ggggcttcct atggatgtga aggtggtggt
     4021 acagaatgtg gggacagccc ttgcggttta tgaagcagcg cggtatggta aacctcttat
     4081 agaaagggtc gtgaccgtga cgggcgaagg gatacagaag ccctctaacc ttatggtaag
     4141 aatcgggacc ctcatttcat cgcttattga ggagtgcggt gggtttaaag atggcgtggg
     4201 caaggtgatc tcaggggggc ccatgatggg gtttgccgtt gcttcccttg acgttcctgt
     4261 aaccaaaggc acttctggaa tactcgtcat ccctgaagag ggggtcctcc atgcagagga
     4321 ctttgagccg tgtatacggt gcggcagatg tattgacata tgtcctatgg gtctcatgcc
     4381 gtccatgctg agtgtctttg ccgaaaaggg ccattatgaa gaggcaaagg agtataacct
     4441 ctttgactgt tttgaatgcg gatcctgtgc cttcgtatgt ccgtcaaaga gacccattgt
     4501 gcagcttgtg aggctggcaa aatctttggt gaaaccataa gaagagagtg agagtaggtg
     4561 gaggaattca agaaagaaca tgagctgatc gtcacggtca gcccccatgt gaagagcgaa
     4621 gagaccgtaa gccgtatcat gtggagcgtg aatctcagtc tcttccccgc atttgccatg
     4681 ggcacctatt tttttggtat caaggcgatc ctggtgactg cgctgtgtat aattaccgca
     4741 atcctttcag agttttttgt ccagaaatcg ctgaaaagaa aggtgaccat acatgacggg
     4801 agtgcctttc ttaccggtct tctcctggga atgaacctcc ccgcgtcgct ctggtcattt
     4861 gcccctttta ccatccatgt gcccatcata gggtcatttg tggcagtggt gattaccaag
     4921 cagctcttcg gaggtttggg atacaacatc ttcaaccccg ccctcatcgg cagggcattc
     4981 cttctcatat cttttcccaa ggtaatgacc atatggaccg agccctcagc agcgctgttc
     5041 gcccttgacg cgaaaacaac cgccacgccc ctgggggtgt tgaaagaaga ggggatagga
     5101 aagcttgtgg aggtctttgg agataaaatg aacctctaca gtcaactctt tgtgggccac
     5161 agggcagggt ccatcggtga gacctcagcc atagcattac ttgtaggcgc agcatttctg
     5221 cttttcaaaa gatacatcac atggcatatc cctctatcgt ttcttggaac cgcagcactc
     5281 ttggcgtggg tattcggcgg caaagatccc caaacaggga aaatgctctt tctcgcaggt
     5341 gaccctatta ttcaccttct gagcggtggt atgcttctcg gtgcgttctt catggcaacg
     5401 gattacgtta cctgtccctc ggtgagaaaa ggacagatac tttttggcat cgggtgtggg
     5461 gccctcacca tgctcataag gctgaaaggc ggatacccgg aaggtgttat gtttgcgatc
     5521 ctcattatga actgttttgc acccattatc gacaggggat ttaagacaaa ggtgttcggc
     5581 acggtgaaaa caagggagac tgcacaatga ccgcaaagga cattatcaag atagccctca
     5641 accttgtggt gatctacgtg atcggcggcc ttatccttgc agccgtgtat gcgaagacat
     5701 ctcccatcat gtacaggaac gcggaggagc aaaaaaaaca ggcattgaaa cagctcatcc
     5761 ctgatgcgga caaaatagag aaattgggcg actggtccat ccatgacaaa cacgcagagt
     5821 atttcattgc caggaaggga gacgagacaa tcggttacat tgtgcaatcc tatggcaagg
     5881 gctattcaag ctatatcaat accctcattg cggtggataa agactttaag gtgcagaaga
     5941 ttaatatact cagtcacgca gagaccccgg ggctgggtga tgaaatagaa gctgactcat
     6001 tcaagggaca gttcaagaac aaggacctgg accatttgaa ggttctcaag acagacacga
     6061 ccgagtacat tcaggcaata tctggtgcca ccatttccag cagggcggtg accgaagatg
     6121 cggttaagaa cggggtcaaa ttccttatgg agaaggtgaa agaaggaggc tcagaaagtg
     6181 gcgcagcaaa gcacggttag ttactgggag ctcatcaaga acggcattat cgcggaaaat
     6241 accatattca aacttgccct cagcctctgt ccctccattg cggtgacgaa taatctaagg
     6301 aacggggttc tgatgggagt tgcggttctg tttgttcaga ccatggtgaa tgccactatt
     6361 tcggtcatga gaaaggtcat ccaccctcgc ataaggcttc ccatttttat gctcgtcatt
     6421 tcgggctggg ttaccattgt ggacatgtcc atggcagcct ttgcgccgga cgcgtacaaa
     6481 cagatgggac tctatatcca gttgattgtt gcctttgcat ccatcctcgc gagagctgag
     6541 atgtttgcga gcaagaacaa gattgctcct tccatggttg acggtatcgg tatggggctt
     6601 gggttccttt tcgcactcac cgtaataagc tttttcagag agctcctggg aaagggttca
     6661 ttatggggca tacccattgt gaatacaaag cctctcctta tcatgatttt gcctgcagga
     6721 ggtttctttg cggtgggaat tttaatggca cttttcaact ggattgatac aaggttttta
     6781 aaaaagggca cctcgggtgc ccactgagtt aacggaggga ttccatgagt ggtgaattca
     6841 ccaagctgtt cgagcttttt attgcggcct ctttgatcaa taattttgtt tttacaaggt
     6901 tccttggact ttgtattttc ttcggtgtct cgaagaagat ggagaccgcc atcgggatga
     6961 gcattacctt tactgccgtg atgatgatca gtgcgtccct gagctggctt atttttacct
     7021 atgttatggt tcccctggac atcacctttc tgaaaatcat cgtgtttatc ggtgtggtgg
     7081 ccgggtttgt tcaggcctcg gacaccatca tgaggaagac cgcgcccgga ctttattaca
     7141 agctgggaat ctatctggcc ctgatatcca ccaactgcat tatccttgct gttccgctga
     7201 ttaacgcggg agagcattac agttttattc agagtattgc cttcggcttt ggttccggga
     7261 tcgggtttgc attggccctg gtaatcatgg cgagtatccg tgaaaaactc gagcttgcgg
     7321 atgtgcctgg acctttcaag gggttgccca ttgcgtttat catcaccggc ctcattgctc
     7381 tggcatttac tggcttctca gggttgatta cattataagc acctatgcta gtataaaatt
     7441 atggagagta agggaatggt ggatcgcctg ataagaagag agtatgcgag tggggactac
     7501 agagagaaaa gaggttcctt ttcagacacc gggttttttg cgcttctgct tctttgtgtt
     7561 ttggcgttct ccctgagcgt tgcttacccc gtttatgccg gggtcggagg aggggttgaa
     7621 gcgaaggagc atgtggaact ccttccttta ttgaaattca cggcaatttt cctggcgggt
     7681 gtgggtttgg tattcggcct tgggctcgcc tttgcggcaa aaaggttttc tgtcaagatc
     7741 gaccctaggg ttgagcaggt gaaagacaac cttgcgcatg cacactgcgg tgcgtgcgga
     7801 tatgcgggct gcgagcaata cgccgaggca gtggtgaaca acccggatgt ccccccgaat
     7861 ctctgtaccc cggcaggtgc gaggggcgct gaggcggtgg cacttattac cgggaaaaag
     7921 gctgaggccc gagaacccat attcgcaagg ataatgtgcc agggaggatg gccgaaggct
     7981 ataaaaaaat ataagtatga aggggttcag gactgccgtg ccgcggtact ggcaggcggg
     8041 ggagacaagg catgtgccta tgggtgcctt ggatacggga catgcgcaag ggtttgtccc
     8101 tttaatgcca tcaccatgaa tgatgaccat atcccggtgg tggatatcac taaatgcact
     8161 ggatgcagga aatgcgaaca ggcttgtccc aagaaggtga ttgaggttct tcctgcctca
     8221 agacaggtac tcgttgcctg ccactccaga gatagagggg ttgatacaaa aaagaactgc
     8281 caggtggggt gcatcgcatg cggtaagtgt gtgaaggtgt gtccctttga tgccccttcc
     8341 atttcctcta acctctcaag gattgacctt gacaaatgtg aggtatgcgg gctatgcgtg
     8401 tctccgtgtc ccacgaacgc aataagagat ttcatcccga agagaccgaa ggcggttgtt
     8461 ttggacaact gtatcggatg ccagatctgc atgaaagtat gtccggtgaa cgcagcctcg
     8521 ggtgagttga agaaaaagca tgccatagac caggataagt gcataggctg tggcatatgc
     8581 acggcaaagt gccctgtgca ggccattgac ggaacattta atgctcatga agtatttcag
     8641 gcagctgctg caaagaaggc cgcaaagact acagccgctt aacccccttt ttgataacca
     8701 ttctttgagc agggaataag ggtctgtcct tttgggaagc agatttgctt atcggaatcg
     8761 gtctctgggt tgcatgcata ttgccaaaaa tgtgtttgat gaattaaaat agataaattt
     8821 cgattgagga ggagtgagtt gaaagaagga attcatcccg attataaaga agcgaaggtg
     8881 gtgtgcgcct gcggtgagac ctttacgacg caatccacaa agccggtgat tcatgtggat
     8941 atctgttcga agtgccatcc ctttttcacc gggaaacaga agctcatgga tgcagagggc
     9001 agggtacaga agttcaagag aaagtacgcg aagaagtagc ggagtacata taaaaggtcg
     9061 tcggtctggg tgagtaggct gacggctttt ttttgacaaa aaaggttgga ggatgaaatg
     9121 aagaatatcg ggggccaggc ggttatcgaa ggcgtgatga tgaagtcgcc aaggggctgg
     9181 accgttgcgg taagagacca aaagggcgac atccacataa agagggaatt ccttaggaaa
     9241 ttacccgggt tccttcacct ccccttcata cgggggtgtg tgggtctcta tcatgctctt
     9301 gccattggca tgaaggcaat agagttttcg gcaagcaagg catacagcac agaagaggaa
     9361 aaacccatga gcaagaccgc tatcgcatcc accatgggag ttgccctcct gatcggtatc
     9421 ggccttttcc tattcctgcc attatatctc acaaagcttg cgggacatgt cgtgggcacg
     9481 attaccacaa gctccctggt ctttaatctt acggacgggg tcatcagggt attcattttc
     9541 ttgttctatg tatcggtcat cgggctgtgg aaggacatga ggaggatatt cgagtaccat
     9601 ggggcagaac acaaggtgat ccatgcatac gaggcaggga agcagttgga tgtcgaaaac
     9661 gtacggatct tcagcccaca tcatcccagg tgcggcacca gtttccttct catcgtaatg
     9721 gtaatgagca tcctcatatt ttccttcatt ccacagtctt ggccttttgg ggggaagttc
     9781 ctgtcgagac ttgtcctgat accggtgatt gcagggacat catatgagct gttaagggta
     9841 tctgcgaaga tgaaagaaaa cacgctcatc catttaatga tccttcccgg attaatgtta
     9901 cagaggctta ccacaaggga gcccgacgac tcccagatag aggttgccat taaggcggta
     9961 aatgaggtat tgcagttaga ggtaaacaat gctccggaag cttgagacca tacaggacaa
    10021 gtacgatgaa ttgaccagac tcctcatgag tccggaaatc ctctccagtc cgcagcagta
    10081 ccagaaatac tctaaggaac agtcggatct ccatcccctg gtggagaaga taggacaata
    10141 caggaagctc ctgaccgata ttgagggtgc ggacgagctt ctcaggggcg gggacgggga
    10201 catgaaggaa ctggcgcagg cggaactgga tgaattaaga gtgaagaggc ct
//
LOCUS       DATKOU010000182         6150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5638257, whole genome shotgun sequence.
ACCESSION   DATKOU010000182 DATKOU010000000
VERSION     DATKOU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5638257"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VMU21_08335"
     CDS             complement(<1..638)
                     /locus_tag="VMU21_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUO77573.1"
                     /translation="MSEGVISITILLVLIILFFYSLLRVITRMRKKRGTSQQKEESQV
                     SFIVGTFHELVAKLKDKERELEELRKKAEERADSIESYNEDILQSVPSGVISLDHDLT
                     ITKMNSAAARILQLHVPSAIGKRYDELFMEPLKGILDGKKTIERGELRYITDSGKRLH
                     LGLAITPLLNSVKETIGRLMVFTDLTELKALESQAELRERLSSLGEMAAGMA"
     gene            complement(635..1405)
                     /locus_tag="VMU21_08340"
     CDS             complement(635..1405)
                     /locus_tag="VMU21_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HUO77574.1"
                     /translation="MTILFFIVFLLGSLIGSFLNVCIHRLPRNLSVIFPSSRCPSCNI
                     PIKTYDNIPLLSYLLLGGRCRYCKTRISLRYPLVEALNALMYVLVLWRFGFGWHMPVL
                     FAFCSAMIVITFIDLDFQIIPDTITLPGIPVGLITASLVLQDPFDRSSILGLGGAIIG
                     LFTGGVLFYVIAVLSKGGMGGGDIKMMAMVGSFMGWKSVLLTTFAGSLLGSCVGIFLM
                     VVKGKGRKTKVPFGPFLAAGALISLFYGQEILSLYLRR"
     gene            complement(1402..1716)
                     /locus_tag="VMU21_08345"
     CDS             complement(1402..1716)
                     /locus_tag="VMU21_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77575.1"
                     /translation="MWKRVKDNLDTGIEKIRWFSSLLNERMKIEISLFKLLYQSTEME
                     KKRAELMKTIGGKVFELRNRQERHVLRDPVILEAMDDIERLDAEIEDTKKKASEIGRI
                     EA"
     gene            complement(1809..3899)
                     /gene="recG"
                     /locus_tag="VMU21_08350"
     CDS             complement(1809..3899)
                     /gene="recG"
                     /locus_tag="VMU21_08350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HUO77576.1"
                     /translation="MDRDPSEFPIQYIKGVGPQRAKLLGRLNIQTAGDALVYLPYRYE
                     DRRNIRKICDLAYARMETATGKIISMDIIKLPKSRVKIFALVISDGTGLLKGKWFNQP
                     YMKKNFALGREVILSGVVKRNSYRGTGFEMDNPEYEFTDDDSENLIHTARTVPVYRTT
                     AGMSVRVLRSLMFNVLSTFADHIRDPLPHEILAKYNLPPLGESIYQSHFPGPDADVEE
                     LNRGESAHLKRLSFDELFHLELGIAVMKQGEMREHGIAFQPRGELVNRLMQNLPFRLT
                     RAQERVFREILDDMKKPYPMNRLVQGDVGCGKTVVALMAMLTAVECGYQAALMAPTEI
                     LSEQHYLNIHALVEKLGLGICLLTGTKTERPLDEIRSGRMDVVVGTHALIQEGVTFKK
                     LGLVVIDEQHRFGVMQRALLRKKARNPDVLVMTATPIPRTLAMTVYGDLDYSVIDEIP
                     PNRTPVITRLFSESQRPHIYDAICSEVRKGRQVFVVYPLIEESENSDLKSALMGREAL
                     GKMFPHFRIGLVHGKMKATERESVMASFKNRELDILVCTTVIEVGVDVPNATMMLIVH
                     AERFGLSQLHQLRGRVGRGSEQSYCFLLAYKPHGEEARRRLEIMVKTGDGFRIAEEDL
                     DIRGPGELFGTRQSGMPDLKIANIVRDAILLESARREAFALIEADPHLDNSPLLKKTA
                     ERFWRDKTDIFKTG"
     gene            complement(3918..5054)
                     /gene="nusA"
                     /locus_tag="VMU21_08355"
     CDS             complement(3918..5054)
                     /gene="nusA"
                     /locus_tag="VMU21_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HUO77577.1"
                     /translation="MTKELCFIIDQMSREKGIPKEILLSTLESALLSAARKRYGGRMN
                     VNLRIDPKTCAITVFETKKVVELVANKDEEITPAEANKIASGKSLGDEVEVPIYLQDF
                     GRIAAQTAKQVIFQRVREAERDVIFKEFKDKVGHIISGVVMRKEKNFYFLNIGKTEAV
                     LPQKDSLPGENLKRGDTARVFIEDVRVTPKGPVIYLSRTNPHFVLELFKMEVPEIAEG
                     LVVIKEIVREPGERTKLAVLSKDHTIDPVGACVGMKGTRVQSIVRELRGERIDIIPWT
                     DDPRILISRALSPATVDKIGINEEEKTAMVVVNDQQLSLAIGKKGQNVKLAMKLTGWD
                     IDILSESEYAKMRMEETETALQDHIKKESQKKDVASQNSEDVSE"
     gene            complement(5051..5506)
                     /gene="rimP"
                     /locus_tag="VMU21_08360"
     CDS             complement(5051..5506)
                     /gene="rimP"
                     /locus_tag="VMU21_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HUO77578.1"
                     /translation="MEEKIHALATRVAEECNFEVVDMQLWAKGKRTLLRVFIDKEGGI
                     TLDNCETFSRRLEALLDVEDPITSPYTLEVSSPGLDRPLKNFKDFKKNLGKLVRIVTK
                     EKVNNQSFFVGRLEGANDQSLRLSLTDGKGEVDIPVTIISRANLELELK"
BASE COUNT         1394 a         1622 c         1443 g         1691 t
ORIGIN      
        1 tgggccattc ccgctgccat ctctccaaga ctggagagcc tctcccgcag ctctgcctgg
       61 gattccagcg ccttgagctc ggtgaggtct gtgaatacca tcaacctgcc aatggtctcc
      121 ttcactgaat tcagaagcgg agtgatcgcg agcccgaggt gtaatctttt gcccgagtcg
      181 gtaatgtacc ggagttcccc cctctcgatg gtcttcttgc cgtcaagaat gccttttagc
      241 ggttccatga aaagctcgtc ataacgtttc cctatggcag acggtacatg caactgcagt
      301 atacgtgcag ctgcagagtt catctttgtg atggtgagat cgtggtcaag gcttatgacg
      361 ccgctgggaa cactctggag aatatcctca ttataacttt ctatactatc tgcgcgttcc
      421 tcagcctttt tgcgcagttc ctccagttcc ctctccttgt ctttgagctt tgccacaagc
      481 tcatggaatg tcccgacgat gaaagaaacc tgcgactctt ccttttgttg ggatgtcccc
      541 cttttctttc tcatccttgt gatgaccctg agcagggaat agaaaaagag gattatgagg
      601 accagtaaga ttgttataga gattacacct tcgctcatct tcgtagataa agggaaagta
      661 tctcctgacc gtaaaataag cttatgaggg cgcctgcggc gagaaacggc ccgaagggta
      721 ctttcgtttt cctgcccttt cctttcacga ccataagaaa gatacccaca caggagccaa
      781 ggaggctccc tgcaaatgtg gtgaggagca cggatttcca gcccatgaag gaaccgacca
      841 tggccatcat cttgatatct ccccctccca tacccccttt gctgagtact gcgatcacat
      901 agaataagac accgccagtg aaaagaccga tgatcgcccc acccagtcca aggattgaac
      961 tccgatcaaa agggtcctga agtaccagag atgcagtgat gagaccgacg ggtataccgg
     1021 gaagggttat ggtgtcaggg attatctgaa aatcaaggtc aataaaggtt atgacgatca
     1081 tggcagagca gaatgcgaag aggaccggca tgtgccatcc aaaaccaaag cgccagagca
     1141 ccaatacata cataagggca ttgagggcct ccaccagagg atacctcagc gaaattcttg
     1201 tcttacaata tctgcatctt cctccaagga gaagatagct caggagagga atattgtcat
     1261 atgtcttgat gggtatgttg catgacggac aacgggatga ggggaatatt acggaaaggt
     1321 ttctcggcag acgatggatg cagacgttta aaaaggaacc gatgagagag ccaagcagaa
     1381 agacgatgaa gaacaatatg gtcatgcctc aattctcccg atttctgaag ccttcttttt
     1441 tgtatcctct atctcggcat ccagtctttc aatgtcatcc attgcctcga ggattacggg
     1501 atccctgagc acatgtctct cctgacgatt cctgagttca aagacctttc ccccgatggt
     1561 tttcatcaat tccgctcttt tcttttccat ttctgttgat tggtagagta gtttaaaaag
     1621 ggaaatctct atcttcatcc gttcgttcag taaggacgaa aaccacctga tcttttctat
     1681 ccccgtatca agattgtctt taaccctttt ccacattctt tcctcctttg tgcccgtgct
     1741 atggacggtg gagaggtcct ggatcaattt tccccttttg tggcgaaagg accattgcag
     1801 gaagtccttc agcctgtctt gaaaatatct gtcttgtccc tccagaatct ctccgcagtc
     1861 tttttcaaca agggcgagtt gtccaggtgg ggatctgcct ctatgagggc gaaggcctcc
     1921 ctccgcgcac tctcaagcag tatcgcatcc cggacaatgt tcgcaatctt aagatcaggc
     1981 atacccgact gccgtgtgcc gaacagttca cccggtcccc tgatatcaag atcttcttca
     2041 gcgatcctga acccatcacc tgtcttcacc atgatttcga gccgtctcct cgcctcttca
     2101 ccgtggggct tgtaggcaag caagaagcag tatgactgct cactccctct gccgaccctt
     2161 ccccttaact gatgaagctg ggaaagaccg aacctctctg cgtggacgat gagcatcatg
     2221 gtcgcattgg gaacgtcaac acccacttcg atcaccgtgg tacaaacaag gatgtcgagt
     2281 tctctgtttt tgaacgatgc catcacagac tctctttccg tcgccttcat cttaccatga
     2341 acaagtccaa tcctgaagtg agggaacatt tttcctaggg cctctctgcc catgagtgcg
     2401 gatttaaggt ccgaattctc tgattcttcg ataagcggat acacgacaaa tacctgtctt
     2461 cctttgcgca cttccgagca tattgcgtca tagatgtgcg gcctctggct ttcactgaaa
     2521 agcctcgtaa ttacaggggt tctattgggg gggatctcat caatcacaga gtagtcaagg
     2581 tcaccgtaga cggtcattgc caaggtcctt gggatagggg tggcagtcat gaccaggaca
     2641 tcaggattcc gggccttttt gcggaggagt gccctttgca tgactccgaa acggtgctgc
     2701 tcatcaatta ccacgagtcc gagtttcttg aaagtgaccc cttcctgtat cagggcatgg
     2761 gtccccacca ccacatccat tcggccagag cgtatttcgt ccaacgggcg ttctgtttta
     2821 gttcctgtga gcagacatat gccgagacca agtttttcaa caagtgcgtg gatgttcagg
     2881 tagtgctgtt ctgacaggat ttccgtcgga gccataagcg ctgcctgata tccgcattcc
     2941 acggcagtga gcattgccat gagtgccacg acggtctttc cgcagcccac atctccctga
     3001 accagcctgt tcatgggata gggttttttc atatcatcaa gaatctcacg aaaaaccctt
     3061 tcttgggccc tcgtgagtct gaagggaagg ttttgcatga gcctgttcac cagttcgcca
     3121 cgcggttgaa aggcgatccc gtgctccctc atctctccct gcttcatgac agcgatgccg
     3181 agttccagat gaaaaagttc atcaaaggag agtctcttaa ggtgggcgct ttcgccgcgg
     3241 ttaagctcct ccacgtctgc gtccggacct gggaaatgcg actgataaat actctccccc
     3301 agggggggca gattatactt cgcaaggatt tcatggggaa ggggatctct tatgtgatca
     3361 gcgaaggtcg agaggacatt gaacatcagg gacctcaaga cccgtacgct cattcccgcg
     3421 gttgttctgt agactgggac ggtccttgcg gtgtggatga gattctccga atcatcatca
     3481 gtgaactcat actcgggatt gtccatctcg aatccggttc cccggtagga attcctcttg
     3541 actacgccgc tcagaattac ttccctcccc agggcaaaat tctttttcat gtagggctgg
     3601 ttaaaccatt tgcccttcag taatcctgtt ccgtccgaaa tcacaagtgc gaatatcttt
     3661 acgcggcttt ttggtagctt tatgatgtcc atggagatga ttttccctgt tgcggtctcc
     3721 atccgggcat aggcgaggtc acagattttc cttatattgc gcctgtcttc gtacctgtag
     3781 ggaaggtaca cgagcgcatc tccggcggtc tgtatgttca ggcggcccag taattttgcc
     3841 ctctgcgggc caactccttt tatgtattgt atcgggaatt ctgaggggtc cctatccatc
     3901 agggctgggt aaagcggtca ctccgaaacg tcttcagaat tttgggaggc cacatctttc
     3961 ttctgggatt ctttttttat gtgatcctga agagccgttt ccgtctcttc catcctcatc
     4021 tttgcgtatt cggattcgct caggatatcg atatcccacc cggtaagctt cattgccagc
     4081 ttcacatttt ggcctttttt gcctattgcc agggagagtt gctggtcgtt caccacaacc
     4141 atggcggtct tttcttcctc gtttatccca attttgtcca cggttgcagg gctgagtgcc
     4201 cgggagatga gtatccgggg atcatctgtc cacggaatga tgtctatccg ctcgcccctc
     4261 aactcacgaa cgatggattg aacccttgtt cccttcatcc cgacgcacgc acccacggga
     4321 tcgatggtat ggtccttgga gagaaccgcg agcttggtcc tctctccagg ctcacgaacg
     4381 atctctttta tcaccaccag cccttctgcg atctcaggca cctccatctt gaagagttcc
     4441 aggacgaaat gaggattggt tcgcgagagg taaatcaccg gtccctttgg ggtgaccctc
     4501 acatcctcga taaagaccct ggctgtatcg cctctcttga gattttctcc cggcaaagag
     4561 tctttctggg gaaggaccgc ttcggttttt cctatgttca gaaagtagaa gttcttctct
     4621 tttctcatta ccactccgct gatgatgtga cccaccttgt ccttgaactc cttgaatatc
     4681 acatctcgct ctgcttctct cacccgttga aagatgacct gcttggcagt ctgggcagcg
     4741 attctgccga aatcctgaag gtaaatggga acctccacct cgtcccccag cgactttccg
     4801 gaagctattt tattggcttc agcgggggtt atctcttcgt ccttgttcgc taccagctca
     4861 accaccttct tggtctcgaa cacggtgatg gcgcaggtct tagggtcgat cctgagattc
     4921 acattcatcc tgccgccgta gcgctttctt gcagcagaga ggagggcaga ttccagggtg
     4981 gaaaggagta tctctttcgg gatacccttt tccctgctca tctggtctat gataaaacag
     5041 agttcttttg tcatttcagc tccaactcca gatttgccct tgatattatg gtgacgggaa
     5101 tatcgacctc tcctttgccg tccgtgaggg acagacgcag tgattggtca ttcgctcctt
     5161 ctagtcttcc cacaaagaag ctctggttgt tgactttctc tttagtcaca atcctcacca
     5221 gcttcccaag attttttttg aagtctttga aattcttcaa tggccgatca aggcccggag
     5281 aagatacttc aagtgtataa gggctggtaa tgggatcctc cacgtcaagg agggcttcga
     5341 gccttctgct gaaggtctca caattatcaa gtgtaattcc gccttctttg tcgatgaaga
     5401 cccttaaaag ggtccttttc ccctttgccc acaactgcat atcaacgacc tcaaagttgc
     5461 actcctcggc cacccgggtg gcaagggcat gaattttttc ttccacactc ctgtccataa
     5521 taaaacaaaa gagtggtttg accactcttt ttagataagg tactctctta ttactataat
     5581 aagaatttaa cggtgaaaaa gcaagatcag gggtggaaac ccacccctga atccttaaac
     5641 tatttccctg gcgctggagc cggtgctgga gccggtgccg gtgcttcagc cggtttctct
     5701 ggggccggag ccggtgccat cggagccggt gctggctttg gctcctcagc cttcttgcaa
     5761 cccaccgtaa gggtaaatgc cactaccaac aataatgcaa gtaacagggc taaaattttc
     5821 ttcactgaac agtcacctcc ttcctaatta agactgtgta aaaccataca attattacca
     5881 catatggaag ataatgtcaa gaaaaaattc ctttgttcat ttaagaacgt atgggaattt
     5941 ttgtttccct ttttgtttct gccttaggcc ttgttctgca gaaggacagg gtgaatccac
     6001 aagcaagcaa gaggtggccg ggtttgtttg gacagctgag agcagttttt aagtgataag
     6061 ctgttctgcc tcatgccgct acctgcatct gaggtagtgt actccagtaa acatcatccg
     6121 gggtcctgcg gtcaaggctt tgatgccgac
//
LOCUS       DATKOU010000183        16319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2167710, whole genome shotgun sequence.
ACCESSION   DATKOU010000183 DATKOU010000000
VERSION     DATKOU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167710"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..645)
                     /gene="nuoI"
                     /locus_tag="VMU21_08365"
     CDS             complement(82..645)
                     /gene="nuoI"
                     /locus_tag="VMU21_08365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HUO77579.1"
                     /translation="MTFRQLVKTVFLTEIFQGMALTLKTFFTHPVTRQYPKEKRPAMP
                     GFRGLHGLVRDQQTGEARCVGCGLCAAICPSQCIHIYTADSLDHKKIVERYEIDVLRC
                     VFCGLCVEACPYAAVVLTEHYEYSDYSREVFYMTKDKLLANWDKYMGWEKNMEYFKRF
                     WHPRTDDLGFPKNQAVFKGSASGKETH"
     gene            complement(642..1610)
                     /gene="nuoH"
                     /locus_tag="VMU21_08370"
     CDS             complement(642..1610)
                     /gene="nuoH"
                     /locus_tag="VMU21_08370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HUO77580.1"
                     /translation="MLYVGVILLKIIVIFGVVMLHVAYASYFERKVIGRMQVRLGPMV
                     VGPYGLLQPIADGLKLFFKEDIIPADADKPVFYIAPIISLFAALSSLAVIPLFNGFVI
                     ANINVGLLFILAMSSLSSYGIIMAGWASNSKYSFLGGLRSSAQVISYEIALGLSLVGV
                     MILAGSLNLTDIVRAQEKYPFGMFLIPQIIGCTIFVISGIAETNRAPFDLPEAESELV
                     AGYFVEYSGMRFALFFVAEYMAMIIIAAVGIICFLGGWNGPFVIPYMPFAWFFVKLWV
                     LLFIYYWVRATLPRYRYDHLMNLGWKVLIPLALTNIVITAIIKVLI"
     gene            complement(1796..2206)
                     /locus_tag="VMU21_08375"
     CDS             complement(1796..2206)
                     /locus_tag="VMU21_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUO77581.1"
                     /translation="MALVKGLLQSREYDPNEMIYREGDKGDSLNIIEKGKVKINKTMI
                     QGDQFCIATLRQGDIFGIMSFLDGSDHDATIVSDQKTKLVILRKSDFDTLSRAHPSIA
                     SKILRRLAIHLASIVRNMNSQYIDLTHLMFRKGK"
     gene            complement(2351..3547)
                     /gene="nuoD"
                     /locus_tag="VMU21_08380"
     CDS             complement(2351..3547)
                     /gene="nuoD"
                     /locus_tag="VMU21_08380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HUO77582.1"
                     /translation="MPKTAKGLETKALTISMGPQHPATHGVLRLVLALDGETVVKCTP
                     YVGYLHRGIEKLAEHMTYLNAIPLTDRLDYISSMANNEGYCLAVERLFGIEAPERAKY
                     IRTMVAEMSRISSHILWLGTHALDIGAMTVFLYSFREREWILDLFEMLCGARLTTTYV
                     RIGGVRNDVSQEFLDSLYKFTEQFPSRIAEYETLIDQNRIWLKRTKRIGVIEADEAVS
                     WGLTGPTLRGSGVPYDVRKYAPYDAYDKVEFDVPVGKNGDVYDRYRCRMEEFRQSNRI
                     IKQCIEKLPKGPIMAPEASKVSLPPKDNVLKDMESMIHHFVLITKGPIAAPEGEIYSA
                     VEAPKGELGFYIVSDGTGRPYRMRVRSPSFVHASVIPKLCEGSLVADVIANIGTIDIV
                     LGECDR"
     gene            complement(3531..4142)
                     /locus_tag="VMU21_08385"
     CDS             complement(3531..4142)
                     /locus_tag="VMU21_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HUO77583.1"
                     /translation="MLNISDGAGGDNRGIMAQTLIAQAIAEGLKERYPDEVKEIKEFR
                     GQVSVVVKKGRIREIMEYLHDTPELCMCYLRDICGVDYLGKKEQRFEVVYQLFCISRR
                     YMLRVRAEVPEDDPTIDSVVEIWDGANWHERECYDMLGIRFNGHPDLRRILMPEDWEG
                     YPLRKDYPMKSDLGDKEWNGLKKIFELSEKNKIYEMGEDAKDR"
     gene            complement(4108..4629)
                     /locus_tag="VMU21_08390"
     CDS             complement(4108..4629)
                     /locus_tag="VMU21_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HUO77584.1"
                     /translation="MIIDGTTALVEVEEGVKVIPAANTIITTIDKVVNWGRVSSLWPL
                     TFGLACCAIEMMTTGSAHYDLDRLGVIFRGSPRQSDVVILAGTISKKMVPVIRTVYDQ
                     MPEPRYVMAMGSCACSGGIFDTYSTVQGADTFLPVDVYIPGCPPRPEAFIQGIIKLQE
                     KIHAEYFRWSRWR"
     gene            complement(4677..5000)
                     /locus_tag="VMU21_08395"
     CDS             complement(4677..5000)
                     /locus_tag="VMU21_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HUO77585.1"
                     /translation="MMIILAFGLGAILVGTIFRPRRPYPEKLRPYESGNPPVGEPRSR
                     FSVRFYIIAMLFVIFDVEAVFLYPWAIVFDEIGLYAFVEMMLFITILVVGYIYAWRKD
                     AFRWD"
     gene            5163..6767
                     /locus_tag="VMU21_08400"
     CDS             5163..6767
                     /locus_tag="VMU21_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77586.1"
                     /translation="MVRKSLNSLAAKLIIAVGTLMTLGSIVFGYIFIRYEENIMMNNL
                     MNYANSTADLVKRGVQHSMLSAEREAIEQTLKVIGNGSDIRDISIIDKKGRVLYSSQE
                     SEVGRSVGKNSPTCQGCHTDNAKPLEVLPGSKVWMVVSDATGAKVLKFIIPIYNEPSC
                     STASCHYHSKTQRVLGILETNFSTSTVDATIKQNRIGTFVFGTLFIMMISFCLCVILY
                     KFVSKPVSLLDEGMQKIGKGDLDHIIEIHSNDEMGLLAKTFNSMASDIKRYRENMENW
                     TKALEEEIKKKTSEIMKAHDQLINAEKLASLGRMAAGVAHELNSPLTGIVTFSHLMLK
                     RIPPENKLDIEDLEVIIEQAERCSKIIKGLLGFSRKTTAEKVFVNINALTESTISMVR
                     NQAKFHNIKFSLNFDDSLPQISTDPNQIQQVFINLLINAADAMNERGQVTVFTRTIPD
                     ASTGRQQIEIEFTDTGPGIPEEHLGRVFEPFFTTKPVGKGTGLGLAVSYGIIKKHGGN
                     IFVRSEVGKGASFHVRLPMESSLEQG"
     gene            6822..7181
                     /locus_tag="VMU21_08405"
     CDS             6822..7181
                     /locus_tag="VMU21_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77587.1"
                     /translation="MTLKKVIEILGAEMVITTADMGTEISSVCSSDLMSDLLHFSTTP
                     KSLLVTGLINKQVVRTAEIADIKAIAFVLDKRPDKETIEMARQKGIALMVTRLSRYTA
                     CGRLYAEGLRSCTGEDR"
     gene            7178..7624
                     /locus_tag="VMU21_08410"
     CDS             7178..7624
                     /locus_tag="VMU21_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77588.1"
                     /translation="MTQKKNIEGSFHLTGGDFTNAGGASSEVKSLLTNLGIAPEIVRR
                     AAIATFEGEMNVIIHAVAGVMHYLVTPEHIRVTLNDMGPGIPDIEQAMQEGYSTAPDW
                     VREMGWGAGMGLPNMKKNADEFRIDSIVGEGTTVEILIKIKEDESL"
     gene            7621..7956
                     /locus_tag="VMU21_08415"
     CDS             7621..7956
                     /locus_tag="VMU21_08415"
                     /inference="COORDINATES: protein motif:HMM:NF018753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HUO77589.1"
                     /translation="MNVQAVVDSLSLEVQGAKGNLEREVTGCYISDLLSDVMAHAQAG
                     DLWITLQTHPNIVAVASLKNLAGIIITNKRIPEEETLRKAEDERIPIAVSPSSTFEVG
                     GMLFKMLRC"
     gene            7950..8693
                     /locus_tag="VMU21_08420"
     CDS             7950..8693
                     /locus_tag="VMU21_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUO77590.1"
                     /translation="MLISFSADLHMHTCLSPCAEIDMTPREIVTHARERGLHIIAITD
                     HNSAENVEATSRVASGTGLLVLAGMEISSSEEVHILGLFESPDQARCMQDVVYAHLPS
                     GENDERLYGHQIIVNEDNEVLGFNKRLLIGATDLSAHEIVNLISSFGGISIASHIDRE
                     AFSVLSQLGFIPEDLRFDAVEFSPRIRRDEAEKKFDALRKFPSVSFSDAHHVTDIGKR
                     STTFTMRAPTFYEIKQAFSHSEGRGLFWD"
     gene            8699..9304
                     /locus_tag="VMU21_08425"
     CDS             8699..9304
                     /locus_tag="VMU21_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77591.1"
                     /translation="MEDLSLHILDIAENSIDAGATRVEIVLEEDTEKDVLSMEVRDNG
                     RGMDENTLAMVADPFFTTKTVRRVGLGLPFLKQASGECEGHFSIASEKGKGTTISVSF
                     RRSHIDRKPLGDMGATLMVLIAGNPSIDFALHYRKDAYHYHLETKPLKEELSGVPINS
                     PEILRIIKEDINKGLTEDVRNDANEPESSDPFNHGARKGGT"
     gene            9305..9682
                     /locus_tag="VMU21_08430"
     CDS             9305..9682
                     /locus_tag="VMU21_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77592.1"
                     /translation="MAKPKILIIDDEAIVRVSCKKCLEPEGYDVDSAEDGIAGLRLVE
                     SNTYDLILTDLKMPDMDGIEFLAKIKETHPKAKVIMITGYSTSEHAELAIKSGAYNYI
                     EKPFTPETLVKAVKEALSSTGKS"
     gene            9904..10767
                     /locus_tag="VMU21_08435"
     CDS             9904..10767
                     /locus_tag="VMU21_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUO77593.1"
                     /translation="MGGRILVVDDEPVVVKSCERTLAPEGYTVDTASNGREAIGKLGK
                     DSFDLVITDLKMPDMDGIELIRWIRNSKPGTGVVVITGYPSQESIKEALGLRILDYLP
                     KPFSPNLLIEVTQKAMEFQKAGEVSVAPPPEEYSEEMVKQLNDIVQKYRKKPGSLIPV
                     LQEAQELIGYLPPPVQRHIAKGLRISVSEVHGVVSFYSFFTMKPKGKHNVRVCLGTAC
                     YVKGAEEIVKKLSEHLSIDVGGITEDKRFSLETVRCLGACGLAPVVVVDKDTHGSVAA
                     ISVNEVLKAYE"
     gene            10782..11165
                     /locus_tag="VMU21_08440"
     CDS             10782..11165
                     /locus_tag="VMU21_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUO77594.1"
                     /translation="MPRLTIDDLKKIKEQYKSTITLREGGYRAKVTVHMGTCGIAAGA
                     RKVMEALLDEITKNGVKDVIVTTSGCAGLCSKEPMATVEIIHEAPVKYVELSDEKIRK
                     IFKEHLMNGHVVEDCALVMGSETAY"
     gene            11237..13105
                     /locus_tag="VMU21_08445"
     CDS             11237..13105
                     /locus_tag="VMU21_08445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HUO77595.1"
                     /translation="METYRSSVMLCGGTGCIAGGSLKIKKSLDAELKNRGLENEINVV
                     LTGCNGFCAEGPVMTVYPEDIFYKKVKETDIPLIVEEHFIKGRPVESLMYKEPIKKQT
                     IPLMKEIPFFSLQVLRALRNKGLIDAEKIEEYIARDGYQAAAKALTQMTPEQIIDEVK
                     KSGLRGRGGAGFPTGLKWEESARAKADQKFILCNGDEGDPGAFMDRSIMEGDPHVVLE
                     GMIIGAKAIGATKGYIYVRAEYPLAVHRLKLAIEQARENGLLGDNILGTGFNFDIEIY
                     QGAGAFVCGESTALMRSIEGKRGMPRPRPPQSAVKGLWEKPTVLNNVETYANVPQIIL
                     NGADWFKTLGTEKSTGTKVFALSGAINNIGLIEVPMGIPLRKIIFDIGGGVPKGRKFK
                     AVQLGGPSGGCIPEALLDTPVTYEDIVQTGAIVGSGGMVVMNDLNCMVSVAKFFLEFT
                     VEESCGKCPPCRVGTTVMLDLLTNITEGKGKEGDIELLEELSRDIITTSLCGLGQTAP
                     NPALTTIKYFRDEYESHIREQWCKTGVCKELCTFYIDEGKCKACGLCKKVCPQKAVSG
                     EKKVPHHIDQSLCVQCRSCYEVCKFDSVQIGPRGMREELKQKDAHRLAEATAGKEG"
     gene            13110..15596
                     /locus_tag="VMU21_08450"
     CDS             13110..15596
                     /locus_tag="VMU21_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUO77596.1"
                     /translation="MIELTINGKKIKTERGTTILQAALKNGIYIPNLCYDRRFRPFGG
                     CRLCFVEIEGQRKLFISCNTPVKEGIVVHTETPKLAKVRRTVLELLLVHHPLDCPICD
                     KAGECTLQDLAFKYGPNQSRFLAERKHEPERIDAPIIERNPNRCILCGKCVGVCIEHQ
                     GVGAINFLGRGFKTKISPAFEETLDCEFCGQCIDACPVGALGSKPYKFRSRVWYMDEH
                     KIICPYCGCGCTTNLSLREGKIIRARGKEGVGINDGDLCTRGRFGFDYIYSEKRLTTP
                     LIRKDGELIPTSWEEALEYVAQRLESIKQSHGPSAIGAIGSQRCTMEDNYMLQRFMRV
                     VMGTDNIDSAARFGYAKVQRAAAMAFGTDLPPIKWTAPLEADFILVVESDITSTLPVW
                     GLNFIKAKKDGAQLVVADPKETKLARNSSVWLQIKPGSGIALLNSMAKVILDESLYDL
                     DEELPLEDKIPATPNYEALMKSLAEFTPAAASKLTGIPEDQIISLARSYAKARKRLLA
                     LTLGSSENTKGMNTLLAAANLVLIMGDNPDTLQVPAEYSNTLGMWKVGVRPLSGGKDA
                     HEMLYKPGAVKALYIMGENPLITFKNTSAVERTLGALEFLVVQDIILTETAKLADVVL
                     PASSWAEKDGIFMGATGLLQEIPKLLAETGQSIPDWKIFRNIARLMNRDLGLKDLTEV
                     RSAITDLVTAPSVGEGTPSFNPVSYELSEKPDTEYPLLLITSNLLQHSGALSVLSKNL
                     ESVVSDAYLQVNPKDAQKFNISDEGFVKVISRRGEVYLKAMISNEMPEGVAFAPTHFP
                     HANINALTHPSLNGEAPTDTIKIELAQTKG"
     gene            15727..>16319
                     /locus_tag="VMU21_08455"
     CDS             15727..>16319
                     /locus_tag="VMU21_08455"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUO77597.1"
                     /translation="MIPYKDDNPTTVFPIVTVGLIALNIAIYLWELVSPLGEQHIALA
                     YGAIPRKLLTLEQNQPFHPLLTIFSSMFLHGGFFHLGGNMLYLWIFGNNIEDRLGHVR
                     FMVFYLLSGVIAAYSHAVTEPHSLIPMIGASGAVSGVLGAYFLLFPHARIHTILFFGF
                     FWQLVRVPALIVIGFWAIIQLINGILTEGLLGQGGVA"
BASE COUNT         4402 a         4096 c         4078 g         3743 t
ORIGIN      
        1 ggacaggatt tctccgggta atcacaagca cagacagaat ggttattgca gcagccagat
       61 agaaaaatat ccccgttgcc atcagtgcgt ctccttcccg ctcgccgagc ccttgaatac
      121 tgcctggttc ttgggaaacc cgaggtcatc ggtcctggga tgccagaacc tcttgaaata
      181 ctccatgttc ttttcccatc ccatatattt gtcccagttg gcgagaagct tgtccttggt
      241 catataaaag acctccctgg aataatccga atactcatag tgctcggtca ggaccactgc
      301 ggcgtaagga caggcctcca cgcacagccc gcagaacacg caccgaagca cgtcaatctc
      361 gtacctctcc acgatcttct tgtggtcaag gctgtcagca gtataaatgt ggatgcactg
      421 ggaaggacat atggcggcac agagtccgca tcccacgcac cgggcctccc cggtctgctg
      481 gtcccttaca agcccgtgaa gccccctgaa ccccggcatt gccggcctct tctccttggg
      541 atactgcctt gtcaccgggt gggtgaaaaa ggttttcagt gtcaatgcca tgccctggaa
      601 tatttctgtg aggaagaccg tcttgaccag ctgcctgaat gtcatatgag cacctttata
      661 attgcggtga ttacgatgtt tgtcagggca agggggataa gcaccttcca tccgaggttc
      721 ataaggtggt cataccggta cctgggcaat gtcgctctta cccagtaata gatgaagagg
      781 agcacccaga gcttcacaaa gaaccaggca aagggcatat agggaattac aaacggtccg
      841 ttccatcctc cgaggaagca tatgataccc acagcggcga taatgatcat tgccatgtac
      901 tcggcaacaa agaagagcgc gaatctcatc ccgctgtact ccacaaaata ccctgccacg
      961 agttcgctct cagcctccgg cagatcaaag ggtgccctgt tggtctctgc gatgcctgag
     1021 atgacgaata tcgtacaacc gatgatctgg ggaatcagga acatgccgaa gggatatttt
     1081 tcctgcgccc tgacaatatc ggtgaggttc aaagaaccgg cgagtatcat cacccccaca
     1141 aggctcagtc ccaaagctat ttcgtagctt atcacctgtg ccgaagacct cagtcccccg
     1201 aggaacgaat acttggagtt ggatgcccat cctgccatga tgattccgta agagctcaat
     1261 gacgacatgg caaggataaa gagcagtccc acattaatat tggcaatgac aaatccatta
     1321 aagagcggga tgaccgcaag ggaagacagg gcagcaaaga gggagattat cggcgcaata
     1381 tagaataccg gcttgtcggc atcagcaggg atgatgtctt ccttgaagaa cagtttaaga
     1441 ccgtctgcaa tgggctggag aagcccgtaa ggtcccacca ccatgggacc gagtctcacc
     1501 tgcatacggc ctatgacttt cctctcaaaa tatgaggcat acgcaacgtg cagcatcacc
     1561 acgccgaaga taacgataat cttcagcagt attactccaa cgtagagcat tgtattatag
     1621 gaaccttctc ccatagtgcc ctctcagatc ctccctaggg aaagtgaaag agaacgtgct
     1681 gcgggattct cccttccccg aaaattgcgt gccattaata aaacaaactc agggattctt
     1741 ttgtcaatct tctcatgccc cgtcatgctc cttttcccct tctctcctct gtgcgtcatt
     1801 tgcccttcct gaacatgaga tgggtgagat cgatatattg gctgttcatg ttcctgacga
     1861 ttgaagccag atggatagca agtctccgca atattttgct cgcgattgat ggatgggccc
     1921 gggagagcgt atcaaaatca gatttccgta atatgaccag tttggtcttc tggtcagaga
     1981 caatagtggc atcgtgatcg ctgccgtcaa ggaaggacat gatgccaaat atgtccccct
     2041 gtctgagggt cgcgatacag aactgatccc cctgaatcat ggtcttattt atcttcactt
     2101 tccccttctc aatgatatta agactgtcac ccttgtcacc ctccctatag atcatctcat
     2161 tagggtcata ttccctggac tgcaacaatc ccttgaccaa agccatctct gacccattga
     2221 gatcttggaa aatgtgattt ctctccatct ttatcaggcg tcctcgctct caagaatatc
     2281 cactctgcat caatgccgcg cacagaggaa aaagaacaac ggcacaaaga atatcgtttc
     2341 cgcagcctaa ctacctgtca cattcgccga gcacaatatc gatggtccca atatttgcaa
     2401 tgacatcagc aacgagggaa ccttcgcaca gcttgggtat caccgaggca tgcacaaagg
     2461 acggtgacct caccctcatc cggtaaggcc ttcctgttcc gtcactcaca atataaaacc
     2521 ccagttcccc tttgggggct tccaccgccg agtaaatctc tccttccggt gccgcgatag
     2581 gtcccttggt gataagaaca aaatggtgga tcatggattc catatccttc aatacattgt
     2641 ccttgggggg aagggatacc tttgacgcct cgggcgccat gataggaccc ttcgggagct
     2701 tttcgatgca ctgttttata atcctgtttg actgtctgaa ctcttccatc ctgcatctgt
     2761 accggtcata cacgtccccg ttcttgccca caggcacatc aaactccact ttatcgtagg
     2821 catcatacgg cgcatacttc ctcacatcat aaggaacccc ggatcccctc agcgtcggac
     2881 cggtgagtcc ccagcttact gcctcgtccg cttcaatgac gccaatgcgc ttcgtccttt
     2941 tgagccatat ccggttttga tcgataagcg tttcatactc cgcgatcctg gacgggaact
     3001 gctcagtaaa cttatagaga ctgtcaagga attcctggga aacgtcgttc ctcactcccc
     3061 cgatccgtac gtaggtcgtg gtgagcctcg caccacaaag catctcaaaa agatcgagta
     3121 tccattccct ttccctgaac gaataaagga acacggtcat tgccccgata tccagcgcgt
     3181 gggtcccgag ccacaggata tggctgctta tccttgacat ttccgcaacc atagtcctta
     3241 tgtactttgc cctctcaggg gcctcgatcc caaaaagccg ttccaccgca aggcagtatc
     3301 cttcgttgtt cgccatactt gagatgtaat ccagcctgtc ggtgaggggg atcgcgttga
     3361 ggtaggtcat atgctcagca agcttctcaa tgccacggtg gagatatccc acatagggag
     3421 tgcacttcac caccgtctca ccatccaacg cgagcacgag cctcagcacg ccatgggtcg
     3481 caggatgctg cggtcccatg ctgatggtta atgcttttgt ttctaggcct ttagcggtct
     3541 ttggcatcct ctcccatttc gtatatttta tttttttctg acagttcaaa aatctttttg
     3601 aggccattcc attccttgtc gccaaggtcg ctcttcatcg gatagtcctt ccggagcgga
     3661 tatccttccc agtcctcagg cataagaatc ctcctgaggt cgggatgtcc gttgaaccgt
     3721 atccctaaca tgtcgtaaca ctccctctca tgccagttcg ctccgtccca aatttcgacc
     3781 acgctgtcta tggtggggtc atcctcggga acctcagccc tgacccttaa catataccgg
     3841 cggctgatgc aaaacagctg gtataccacc tcaaagcgct gctccttctt gccgagataa
     3901 tctaccccgc aaatatcccg caggtagcac atgcaaagct caggcgtatc atggaggtac
     3961 tccattatct cccgtatccg ccccttctta accactaccg acacctgacc cctgaactcc
     4021 ttgatctcct tcacctcatc cggataccgt tctttcagtc cttcagcaat ggcttgagct
     4081 atgagtgttt gcgccatgat ccccctatta tctccacctg ctccatctga aatattcagc
     4141 atgtatcttt tcctggagtt tgatgatccc ctggatgaac gcctctggtc ttggcgggca
     4201 accgggtata tacacgtcaa caggcaaaaa ggtatcagcg ccctgcaccg tactgtaggt
     4261 gtcaaatatt ccccctgaac acgcacagct ccccatcgcc atcacatacc ttggttctgg
     4321 catctggtcg tacactgtcc tgattacagg gaccatcttc tttgatatcg tcccggcaag
     4381 gatcaccaca tccgattgcc tgggtgaacc tctaaagatc acccccagcc tgtccaagtc
     4441 gtagtgcgca gatccggttg tcatcatttc aatagcacag caggccaagc cgaaggtcaa
     4501 aggccagagg gaagagaccc tcccccagtt cacgacctta tcgatagtcg tgataatggt
     4561 gttggcagca gggattacct ttaccccttc ctctacttca actaatgcgg tggtaccgtc
     4621 aattatcata tgctcacctt tctattggcc tacggttcat cgctgatggc tcggggctaa
     4681 tcccacctga aggcgtcttt cctccatgca taaatatagc ccactaccaa tatggtaatg
     4741 aagagcatca tctccacaaa tgcatagagc ccaatctcat caaatacgat ggcccatgga
     4801 tagagaaata ccgcttccac atcaaagatt acgaagagca tggcgatgat gtaaaacctt
     4861 accgaaaacc tggaccgcgg ctctcccaca gggggattgc ccgactcgta ggggcgaagc
     4921 ttctcaggat agggccttct gggcctgaat atggtgccca caaggattgc cccaagacca
     4981 aaggccaata taatcatcat aaagatgata accggcagat actcttctgg taaaaaggct
     5041 ctcggcataa aaacgcacta ttttatacct aaagttatca ctggagtcaa gtaaaaaatc
     5101 tcactcagaa attgagcggt caggaatgag aattttgctg ccttttatga taaaattatc
     5161 gaatggtccg gaaatctttg aactccctcg cagccaaact catcatcgca gtgggcaccc
     5221 ttatgacatt aggcagtatc gtgtttggat atattttcat ccggtacgaa gaaaacatca
     5281 tgatgaacaa tctgatgaat tacgcgaatt ctaccgcaga tcttgtgaaa cgcggggtac
     5341 agcacagcat gctttctgcg gagcgggaag ccatagaaca aacactcaag gtcatcggca
     5401 acggttcaga tatcagggat atctcgatta tagataaaaa gggaagggtt ctgtattcct
     5461 cacaagagtc agaggtgggt cgctcggtcg ggaagaactc ccctacctgt cagggttgcc
     5521 atacagacaa cgcaaaaccc ctcgaagtcc ttcctggatc aaaagtctgg atggttgtct
     5581 ctgatgccac tggtgccaag gtcctaaaat tcatcattcc catctataat gagccctcgt
     5641 gttctactgc ctcgtgtcat tatcattcca agacgcagcg tgtcctcggg attctggaaa
     5701 cgaatttcag cacctccaca gtagacgcca ccataaaaca gaaccgcatc gggacctttg
     5761 tgttcggcac tctcttcatc atgatgatat ccttctgcct ttgcgtcatt ctctataagt
     5821 ttgtatcaaa acctgtttct ctcctggatg aaggcatgca gaaaatcggg aagggtgatc
     5881 tcgaccatat tatagagatc cattccaatg acgaaatggg cctgctcgca aagacattca
     5941 attctatggc gagcgatatc aagagataca gggaaaacat ggaaaactgg accaaggcgc
     6001 tggaggagga aatcaagaaa aagacctcag agatcatgaa ggcccatgac cagctcatca
     6061 atgcagagaa gctcgcctca ctcggcagaa tggcagcggg tgtggcacac gaactgaata
     6121 gtccgctgac aggaatagtc acgttttccc atctcatgtt aaagcgcata cctcccgaga
     6181 acaagctgga tatcgaagac ctggaagtca tcattgagca ggcggaacgg tgctcgaaga
     6241 taatcaaggg gctcctcggt ttttcacgaa aaaccacggc cgaaaaggtc ttcgtgaata
     6301 taaacgccct tacagagagc acaatatcca tggtgagaaa tcaggctaag ttccacaata
     6361 taaagttcag tctcaatttc gatgactccc ttccccagat ttcgacggat ccgaaccaga
     6421 tccaacaggt cttcattaat ctccttatca atgcggccga tgcgatgaat gagcgcggac
     6481 aggtcactgt ctttacaaga acgatccctg atgcttccac tggaagacag caaatcgaga
     6541 tagagttcac cgatacagga ccagggattc ccgaagaaca tctcggaagg gtatttgagc
     6601 cgttctttac taccaaaccc gttggtaaag gaacagggct gggacttgcc gtaagttatg
     6661 ggatcatcaa gaagcatggg ggaaatatct ttgtgagaag cgaagtgggc aaaggggcga
     6721 gctttcacgt aagattaccc atggaaagca gtcttgaaca ggggtaagga cttgtcctgt
     6781 agctcacgaa gacgatatat tacatcacat aaaataagag catgaccctt aagaaagtga
     6841 tagaaatcct aggcgctgag atggtaatta ccaccgcaga tatgggcact gaaatttcat
     6901 cggtgtgcag ttcagatctc atgagcgacc tcctccattt ctccaccacc ccaaaatccc
     6961 ttcttgttac cggcttgatc aacaagcagg tcgtcagaac cgcggaaatc gcggacataa
     7021 aggcaattgc ctttgtcctt gacaagagac ctgataagga aaccattgag atggcaaggc
     7081 aaaagggtat tgccctcatg gtaacgaggt tgtcgaggta tactgcatgt ggcagactct
     7141 atgccgaagg gctcaggagt tgcaccggag aagacagatg acacaaaaga aaaatatcga
     7201 agggagtttt catctcacag gaggagattt taccaatgcc ggtggggcat cgagcgaagt
     7261 gaagtccctc cttaccaacc tcggcattgc tcctgaaata gtgagaaggg ctgccatcgc
     7321 gacctttgag ggcgaaatga atgtgatcat ccacgcggtc gctggggtga tgcactatct
     7381 cgtaacccct gagcatatca gggtcaccct gaacgatatg gggccgggaa tccccgatat
     7441 cgagcaggcg atgcaggaag ggtattctac tgccccggac tgggtgagag agatggggtg
     7501 gggtgccgga atggggttgc caaacatgaa aaaaaacgcc gatgaattca ggatagattc
     7561 cattgtcggc gagggaacga ccgtcgagat actcataaag attaaggagg atgaatcatt
     7621 gtgaacgttc aagcggtagt cgacagcttg tccttggaag tgcagggtgc aaagggaaat
     7681 cttgagcgtg aagtcaccgg ctgttacatc agtgatcttc tctccgacgt catggcacat
     7741 gctcaggcag gagatctgtg gattactctt cagacacatc ccaatatcgt cgcagtggca
     7801 tctctcaaaa acctggccgg aattattatt accaacaaga ggatccccga agaagagaca
     7861 ctgagaaaag cggaagatga gcgtattccc attgcggtaa gcccctcatc cacgtttgaa
     7921 gtggggggca tgcttttcaa aatgcttaga tgctgatctc tttttctgca gacctgcata
     7981 tgcatacatg cctttccccc tgtgctgaaa tcgatatgac cccgcgagag atcgtcacgc
     8041 acgcgcggga aagaggtctg cacatcattg ccattactga ccataattct gccgagaatg
     8101 tggaggcaac aagcagggtg gcctcaggga cagggctcct ggttctggcg gggatggaaa
     8161 tttcctcctc agaagaggtg catatcctcg ggctcttcga gtcccctgat caggccaggt
     8221 gcatgcagga cgtagtatat gcacatcttc cctctgggga gaacgatgag agactgtacg
     8281 gacaccagat tatcgtcaat gaggacaacg aggtgctggg atttaacaag agattgctca
     8341 ttggggctac agatctcagt gcgcatgaga tagtaaatct tatcagttct tttggcggga
     8401 tatctatagc cagccatata gaccgggagg cattcagtgt gctctctcag ctcggtttta
     8461 ttcccgagga tctgcgcttt gacgcggttg agttttctcc ccggatacgc agggatgaag
     8521 cggagaagaa gttcgacgca ctgaggaaat tcccgtcggt ctccttttca gatgcacatc
     8581 acgtcactga tatcgggaaa aggagtacca ccttcaccat gagggcaccg actttttatg
     8641 aaataaaaca ggcattcagc catagtgaag ggagaggcct cttctgggat tagcccgcat
     8701 ggaagatcta tcattacata tccttgacat cgctgagaac tcgattgatg cgggtgcaac
     8761 gagagttgaa atcgtactgg aagaagatac tgaaaaggat gtactctcca tggaggtgag
     8821 ggacaacgga cggggcatgg atgaaaatac actcgcgatg gttgccgacc ccttctttac
     8881 aacaaaaact gtgaggaggg ttggccttgg attgccattc ctcaagcagg cttccgggga
     8941 gtgcgaaggc cacttttcca ttgcatcgga gaaaggaaaa ggcacgacca tttcggtgag
     9001 cttcagacgc agccacatag acaggaaacc gctgggggat atgggcgcca cattaatggt
     9061 gctcatcgcg ggtaatccat ccatcgattt cgccttgcat tacagaaagg atgcttacca
     9121 ttaccacctg gagacgaaac cgctcaaaga ggaactgtcg ggcgtaccga taaattcacc
     9181 ggaaatattg aggataatca aggaggatat caacaaagga cttacagaag acgtgaggaa
     9241 tgacgcgaac gaaccggaat cttcagatcc ttttaaccat ggggctcgta agggagggac
     9301 atgaatggct aagccgaaga tcctgatcat tgatgatgaa gcgattgtga gagtaagctg
     9361 caaaaagtgt ctggagccgg aaggctatga tgtcgatagc gcggaagatg gtatcgcagg
     9421 actccggctt gtggaatcaa acacctatga ccttatcctg acagacctca agatgcctga
     9481 catggatggc atcgaattcc ttgccaaaat aaaggagaca catcccaaag caaaggtaat
     9541 catgataacg ggatacagca cgtcggagca tgctgaactg gcaataaagt caggggccta
     9601 caactacatc gagaaaccat ttacccctga aacgttggta aaagcggtaa aagaggccct
     9661 gagcagcacg gggaaatctt aacacagaag accgtttgga ctatcagggg gcgccgcttg
     9721 ataccgtggt tctttgcccc ttattcagca actccgtgct gcccgcatat tctaagctgc
     9781 tcgttttttt atctgcaggt acctcagttt cagagaggta acgggacggt ccggcaatgc
     9841 ttgaaaaagg aaagagcctt tttatatgat agtgcatatg taggtttcaa ggagggagaa
     9901 ggaatgggag gcagaatact tgttgtggat gatgaacccg ttgtcgtgaa gagctgcgag
     9961 cgtaccctgg cccccgaggg gtataccgtg gataccgctt caaatgggag agaagccata
    10021 ggaaaactgg ggaaagacag cttcgatctt gttatcacag acctcaaaat gcctgacatg
    10081 gatggcatcg aacttatcag gtggataagg aattcaaagc ccggtacagg ggtcgtggta
    10141 ataaccggat atccttccca ggaaagcatc aaagaagccc tcggcctcag gatactcgat
    10201 tatctgccta agcccttttc cccaaatctc ctcattgaag ttacgcaaaa ggccatggaa
    10261 ttccagaaag caggagaggt ttcggtggca cctcccccag aggaatactc cgaggagatg
    10321 gtgaaacagc tcaatgacat agtacaaaaa taccggaaaa aaccgggaag cctgatcccg
    10381 gtgcttcagg aagctcagga gctcatcggc tatcttcccc cgcctgtgca gcgccacatt
    10441 gccaagggat tgagaatctc cgtaagcgaa gtccacggag tcgtatcttt ctattccttc
    10501 ttcacaatga aaccaaaagg caagcacaat gtgcgcgtat gccttggaac agcgtgttat
    10561 gtgaagggtg cggaagaaat agtcaaaaaa ttgagcgaac acctctctat tgatgtaggg
    10621 ggcattaccg aagacaagag gttctctctc gaaacagtac gatgcctcgg tgcatgcgga
    10681 ctcgcccctg tggtggtggt ggataaggac acccacggtt cggtggccgc catcagcgtg
    10741 aatgaagtat tgaaggctta cgaataagag gaggaggtac aatgccgcga ctgaccatcg
    10801 atgatctgaa aaagataaag gagcagtata aatcaaccat caccctgaga gagggtggct
    10861 acagggcaaa ggtgacggtc cacatgggca cgtgtggtat tgctgcaggc gcaagaaaag
    10921 ttatggaagc attgcttgat gagattacca agaatggcgt caaagacgtg atcgtaacca
    10981 catcaggatg tgcagggctc tgcagcaaag agcccatggc aaccgttgag atcattcatg
    11041 aagcgccggt gaaatacgtg gaactgagcg acgaaaaaat aagaaaaatc ttcaaagagc
    11101 acctcatgaa cgggcatgtc gtggaagatt gtgcccttgt aatggggagt gaaacggcat
    11161 attaaacggc agcgggtagc agggaccaaa agacggtatt ctgcaccaca cccttaccaa
    11221 tcatagagga ggagcgatgg aaacatatcg ttcgagtgtc atgttatgcg ggggcactgg
    11281 ctgcatcgca ggcggtagtc tcaagataaa aaaatctctc gatgcggagt tgaaaaatag
    11341 agggctcgaa aatgagataa acgttgttct caccgggtgc aacggttttt gcgcagaagg
    11401 gccggtcatg accgtgtacc cagaggacat cttttataag aaggtcaaag aaacggatat
    11461 tccccttatt gttgaggagc atttcatcaa gggaagaccc gtggaaagcc tcatgtacaa
    11521 ggaacccata aagaagcaga ccatccccct tatgaaggaa atacctttct tcagccttca
    11581 ggtgctccgg gccttgagga acaaggggtt gatagatgcg gaaaaaatcg aggaatacat
    11641 cgccagggac gggtatcagg cggctgccaa ggcattgacc cagatgaccc ccgagcagat
    11701 catagatgag gttaagaaat ccggtctccg gggaagaggc ggagcagggt ttcccactgg
    11761 gttaaagtgg gaggagagcg caagggccaa ggcagaccag aagtttattc tctgcaacgg
    11821 tgatgagggt gaccccggtg cgttcatgga caggagtatc atggagggag atccccatgt
    11881 cgtgttagag ggtatgataa tcggcgccaa ggccatagga gcaaccaaag gatatatata
    11941 tgtgagggca gagtaccctc tcgcagttca ccggctcaaa ctcgcgatag agcaggcaag
    12001 ggaaaacgga ttgttgggtg acaatatcct ggggaccggc ttcaactttg atattgaaat
    12061 atatcaaggg gcaggcgcct tcgtgtgcgg tgaatccact gccctcatga ggtccattga
    12121 aggcaaaagg ggcatgccgc ggcccagacc tccccagtca gcggtgaagg gattatggga
    12181 aaaacccact gtccttaata atgtggaaac gtacgcgaat gttccacaga tcattttgaa
    12241 cggtgcggac tggttcaaga ccctcggcac ggaaaaatca accggcacaa aggtctttgc
    12301 cctgagcggc gcaataaaca acatcggcct catcgaagtt cccatgggaa tccctttgag
    12361 gaagatcata tttgatatcg gcggaggcgt gcccaagggg aggaaattca aggcagttca
    12421 gcttggggga ccctcgggag ggtgcattcc cgaagcactc ctcgacaccc cggtcactta
    12481 tgaagatatc gtacaaacag gagccattgt cggatcaggc ggaatggtgg tgatgaacga
    12541 cctcaactgt atggtgagcg tggcgaaatt tttccttgaa tttaccgtag aagaatcatg
    12601 cggcaaatgc cccccttgca gggtcggcac cacggttatg cttgacctgt tgaccaatat
    12661 aactgaaggc aaggggaaag aaggggacat agaactcctt gaggagctct cgcgcgacat
    12721 cattaccaca tctctctgcg gtctcggcca gactgctccg aacccggccc tgaccaccat
    12781 aaaatatttc agggacgaat acgagtccca tataagggaa caatggtgta aaacaggggt
    12841 atgcaaggaa ctctgcacct tttatattga tgagggaaaa tgtaaggcct gcggtttatg
    12901 caaaaaggtc tgcccgcaaa aggcagtctc aggagagaag aaggtgcccc accacattga
    12961 ccagtccctt tgcgtgcaat gtcgctcatg ctatgaggtc tgcaagttcg actcagtgca
    13021 gatagggcca agaggaatga gggaagagtt aaagcagaaa gatgcacatc gactcgcaga
    13081 agcaacggca ggcaaggagg gataaacgca tgatcgaact caccataaac gggaagaaaa
    13141 taaagacaga acggggaacg accatcctgc aggcagccct taagaatggc atctatattc
    13201 ccaacctctg ctatgacaga aggttcaggc ccttcggagg ctgcaggctc tgttttgtgg
    13261 agatcgaggg acagaggaag ctcttcatat cctgcaatac gcctgtcaaa gaaggaatag
    13321 tggtccatac agaaacccca aaactggcaa aagtgagaag gaccgtcctg gaacttctcc
    13381 tcgtacacca ccctctcgac tgccccatct gtgataaggc cggggaatgc actctccagg
    13441 accttgcgtt caagtacggc ccgaaccaga gtaggtttct tgcggagaga aagcatgaac
    13501 ccgagagaat cgacgccccc attatagaga ggaatcccaa cagatgtatt ctgtgcggca
    13561 aatgcgtggg tgtatgcatt gaacatcagg gagtgggagc aatcaatttt ctcggaaggg
    13621 ggtttaagac aaagataagc cctgcttttg aagagaccct ggattgcgaa ttttgcggtc
    13681 agtgcataga cgcgtgtccc gtgggggcac tcggaagcaa accatacaag ttcaggtcaa
    13741 gggtctggta catggatgaa cataagatta tatgccccta ctgcggatgc gggtgcacca
    13801 caaacctcag tctcagggag ggaaagataa ttcgtgccag ggggaaagaa ggcgttggga
    13861 taaatgatgg cgacctttgc accaggggga ggtttggctt tgactatatt tactctgaaa
    13921 agagactgac cacccctttg ataagaaaag acggggaact catccccacc tcctgggagg
    13981 aggccctgga atatgttgct cagcgtcttg aatcaatcaa acaaagccac gggccttcag
    14041 ccattggcgc tatagggtcc cagagatgca ccatggaaga taactatatg ttgcagcgct
    14101 tcatgagggt ggtcatggga acggataaca tcgactccgc ggcccgcttc ggctatgcaa
    14161 aagtgcagag agcagcagcc atggcttttg gaacggatct tccccccata aagtggaccg
    14221 cccctttgga ggcagacttt attctggttg tggagtctga tatcacatcc acccttcctg
    14281 tgtggggttt gaatttcatt aaagccaaga aagatggcgc acagcttgtg gtcgcagatc
    14341 caaaagaaac caagctggca aggaacagct cagtctggtt acaaataaaa ccgggatcag
    14401 gcattgccct ccttaacagc atggcgaaag taatccttga cgaaagcctc tatgaccttg
    14461 acgaggaact gccgctggaa gataagatac ccgccacgcc caattatgag gcactgatga
    14521 aaagccttgc tgaattcact cctgcagctg cgtcaaagct tacgggcatt cccgaggacc
    14581 agatcatcag cctcgcaagg tcctatgcaa aggcccgcaa gaggctcctt gcgttaaccc
    14641 tcggttcctc cgaaaataca aagggcatga ataccctcct tgctgccgca aatcttgtcc
    14701 tcatcatggg tgacaatcct gacacactcc aggtgcctgc cgagtactcc aacacgctag
    14761 gcatgtggaa ggttggtgtc agaccccttt ctggcggcaa ggatgcccat gagatgctgt
    14821 ataagcccgg agcagtaaag gctctctata tcatgggaga aaacccgctc atcacattca
    14881 aaaatacatc cgctgttgaa aggacacttg gtgcacttga gttcctcgtg gtacaggata
    14941 tcattctcac cgaaactgca aaactcgccg acgtcgttct tcctgccagt agctgggctg
    15001 aaaaagatgg catattcatg ggagccactg gccttctcca agagattcca aagctcctcg
    15061 cagaaacggg acaatccata ccggactgga agatattcag aaacatcgca agactcatga
    15121 acagggacct tggcttaaaa gaccttaccg aggtgaggtc agccattaca gatcttgtta
    15181 cggctccatc cgtcggggag ggaactccct cgtttaaccc tgtttcatat gaactgtctg
    15241 aaaaaccgga cacagagtat cccctgctcc tcatcacgtc gaacctgtta cagcactccg
    15301 gtgcgctctc ggtactttca aagaatctgg agtcagtggt ttctgatgcc tatttgcagg
    15361 tgaacccaaa agatgcccag aaattcaaca tctcggacga agggttcgta aaggtcatat
    15421 cacggagggg ggaagtttac cttaaagcaa tgatctccaa tgaaatgcct gaaggagtcg
    15481 cctttgcccc cacgcatttt ccccatgcca acatcaatgc actgacccac ccttccttaa
    15541 atggcgaagc gccgacagac acgatcaaaa ttgagttggc acagacaaaa ggctgaagga
    15601 gaagcaacgc gggatggagg aggaaaagtg aatggatcat gttcctgtcc tgccttcatc
    15661 ccttgaacct ctctttgccg tctttcacct tccttctttt caagcctcat cacagggcaa
    15721 ggtcggttga ttccctataa ggatgacaat cccaccaccg tctttcccat cgtaacggtg
    15781 gggctgatag ctctcaatat cgcaatatac ctatgggagc ttgtatcgcc gctaggagaa
    15841 caacacatag ccttggcata cggtgcaatt ccgcgcaaac tgctcacgct tgagcaaaac
    15901 cagccttttc acccgttgct caccatcttt tcgtcgatgt tccttcacgg agggttcttc
    15961 catctcggag gcaatatgct ttacctttgg atcttcggca ataacattga ggacaggctc
    16021 ggacatgtac ggttcatggt cttttacctt cttagcggtg taatagctgc gtattcccat
    16081 gcagtaactg aaccacattc tttgatcccg atgatcggtg ccagcggagc agtatcagga
    16141 gtccttggcg cctatttcct gctgttcccc cacgcacgca ttcacaccat actctttttt
    16201 ggattcttct ggcaactggt gagggttcct gccttgatag ttataggatt ttgggctatt
    16261 atacaactga ttaatggaat tcttaccgaa gggttattgg gccagggcgg agtcgcatg
//
LOCUS       DATKOU010000184        31617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2562673, whole genome shotgun sequence.
ACCESSION   DATKOU010000184 DATKOU010000000
VERSION     DATKOU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 31617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31617
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562673"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VMU21_08460"
     CDS             <1..262
                     /locus_tag="VMU21_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HUO77598.1"
                     /translation="MWEKVGIIRCAESLSVAGDIISRWLPSVGGASIKREELELKNMI
                     TVASLITEAAMARRGSVGAHYRSDYPARGESWQQHRTWQKPE"
     gene            complement(536..1900)
                     /locus_tag="VMU21_08465"
     CDS             complement(536..1900)
                     /locus_tag="VMU21_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO77599.1"
                     /translation="MDPLVLIVDDEEGIRESVSAILEDERYRVATAGSGEDALVILQE
                     QFPDLIILDVWLPGMDGIQTLQEIAALKRGTPVIMISGHGNIEVAVKATRMGAYDFLE
                     KPLSLERIVLSARRALERSALERENRDLKENLTRKWRLIGDSSPMKTLHAQIEMAAQS
                     NSRVLITGESGSGKELVARLLHAKSTRGERPFVELNCAAMPYELIESELFGHEKGSFT
                     GAFEKKKGKFELADRGTLFLDEIGDMSLQTQSKVLRVIETQEFQRVGGNTTIKVDVRI
                     IAATNKDLTEEVKRGNFRDDLFFRLNVVPIVVPPLREHKEDIPALVDHFIKSLATEYG
                     QPPKRIMADAIKSLQEYNWPGNIRELKNLIERLAIMTPSADIHARDFRLAGRHEGADF
                     FSYGTLKQAREAFERDFILRKLEENNWNISRTAEILDIERSNLHRKIKAYHIQAGEDT
                     APKS"
     gene            complement(1902..3935)
                     /locus_tag="VMU21_08470"
     CDS             complement(1902..3935)
                     /locus_tag="VMU21_08470"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77600.1"
                     /translation="MKKLGMILGGLLLFVILVSTVELHLMRQASLSITTRVTLLILLN
                     VNIIALLTLMFFVAKALTKLYLEKKRKILGSRFKTKIVVVFVILTSIPSVLLFLGASG
                     LVTNYIDRWFTPQFRQPIDSTLVIARALYDTERKKTLEAARSIVSKGTIPMEYTVTHL
                     SELPDNPSDVVTEAFGGEEGTEVISGENGDTVRAVVPDSTGGRRSGVVIVESHIPRDI
                     TRNVEKIKRAYEDYIQLEAWKQPLKLNYQLFLGFFTLIVIFTALWVSLRVARGISEPI
                     QMLAEATGEIASGNLDVDIALKRDDEIGLLINSFNHMVKELKEGKASLQKAYVESDRR
                     RLFMENILENIQSGVISLDADGTILAMNTAACAILNVALENVMGKNYKELLSLLRSDS
                     LQEMIRGIKLRTLGMVEKEIWATIKGKRTMLRVSIMGLRNNASEYLGLLVVFDDLTNV
                     IKAQKALAWQEVARRIAHEVKNPLTPIKLSTERMLKKWERKDEDFPQVFERSMRTIIK
                     EVDSLRNLVDEFSRFEKMPEIHKSSTILAGIINEVVNLYRDYKGFSIRVSLPEDMPPI
                     DMDREQFKRVLINLFENAIQAMHSGGNIEVTVHLNVSSNRVFMDVADNGPGIREEDKE
                     KLFLPYFSTKKEGTGLGLAIANKIIREHMGHLRVRDNEPQGTVFTIEMPIKEG"
     gene            complement(3932..4489)
                     /locus_tag="VMU21_08475"
     CDS             complement(3932..4489)
                     /locus_tag="VMU21_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HUO77601.1"
                     /translation="MARIILSLAFLSLFLMLLNASGAEIIGPDARVTNDEIVVSTGLA
                     LDEKNVDDLKNGISKEITFYIDLFRVWRMWPNEFIAGKKIVKTLRSDPIKKEYTATTF
                     DGATLTEKRFKRFDSMLDWTLTIKDLKLINIKELQSSDYYVKVTVESRLRELPPVIGY
                     LLFFVSEKDFKLSKESPSLSTGTEK"
     gene            4598..5080
                     /locus_tag="VMU21_08480"
     CDS             4598..5080
                     /locus_tag="VMU21_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HUO77602.1"
                     /translation="MELEAIRIEIPEECNIILGQTHFIKTAEDLYEIVATTVPQAKFG
                     IAFTEASGPCLIRTEGNDGMLINCCVAMLKDIGAGHVFCVLLKDAYPINVLTQIKSCQ
                     EVCRVYCATSNPLEVLVASTKQGKGIIGVVDGFPPRGVETSDDRLRRREFLRTIRYKA
                     "
     gene            5165..5596
                     /locus_tag="VMU21_08485"
     CDS             5165..5596
                     /locus_tag="VMU21_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUO77603.1"
                     /translation="MSDEAKSASLLLRVFAKILDFILIAAMAEVIPKAGFYAGLSYLL
                     ISDGLFEGRSIGKLLIGLRVISTAHDGPCSMRESIVRNAPLGAGLLFFKLPWVGWIVI
                     AGVSAVEFLILLGSKDGRRLGDELAKTTVVELVKSKDSQEV"
     gene            5596..6627
                     /locus_tag="VMU21_08490"
     CDS             5596..6627
                     /locus_tag="VMU21_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUO77604.1"
                     /translation="MLFHKVVGMFSNDLAVDLGTANTLVFVKGKGIVCNEPSVVVVRK
                     EDKKTIAVGADAKRMLGKTPSNIIAIRPMKDGVIADFDATGEMLKYFIKKVHNRKSFL
                     SPRVIIGVPSGITQVEQRAVKDAAQASGAREVYLIEEPMAAALGVGLPVGEPSGNMIV
                     DVGGGTTDIAVISLDGIVYSKAVRVGGDKMDDALIAYIKRKYNLLIGDRTAELIKIEI
                     GSACVVDPGVTRTMEIKGRDLISGIPKIILVTETEIREALHEPVSVILDTIKITLENT
                     PPELAADIVDRGIVLAGGGALLRGLDILIREETGLPVIVPDDPLTAVARGVGKMLDEL
                     ELLKKIAIT"
     gene            6627..7409
                     /gene="mreC"
                     /locus_tag="VMU21_08495"
     CDS             6627..7409
                     /gene="mreC"
                     /locus_tag="VMU21_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HUO77605.1"
                     /translation="MSIRRLFPLFLLVVLSLTLMTYQSNKGPIAPFRFLSNPLNYLND
                     MIHSISSSLKEPFRKIMLRDEDMKKLKGQIESLLLEQQRYRDLFLENQRLREILSVKE
                     RERRYVATARIISRGPDLWSNMLVIGKGTREGIAKDMAVITPLGLVGKISVVTDHYAY
                     VLLVTDISFSAAVRIQDTREEAILSGTGTRRCVLQYIPEEALIREGEMVVTTGFDDVF
                     PQELIVGYVSRVSKKGSSIFQQVEVTPSQDLTKLDEVIIVRR"
     gene            7406..7858
                     /locus_tag="VMU21_08500"
     CDS             7406..7858
                     /locus_tag="VMU21_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77606.1"
                     /translation="MKSFIIFLGVALAFLLQTKISFLGVSPDLTAAVAYYFGITGGST
                     KGVLFGSLIGVIEDSVGGNILGPNLLGKGMVGFFSSFMSGSLFRWTPFLGVVSLVILT
                     LTDGSIILLSRAIFDAMPTSMSSGIFTLFMQGLFNGIIGIFIRPRNVD"
     gene            7848..9629
                     /gene="mrdA"
                     /locus_tag="VMU21_08505"
     CDS             7848..9629
                     /gene="mrdA"
                     /locus_tag="VMU21_08505"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUO77607.1"
                     /translation="MSINGNSSEKVLISAYLIIALFFILTIRIWQLQILQGSEYRKLS
                     EENRLRIVRVAAPRGIIFDRNGIPLVKNTPYYFVSISPQNLDGLDISALAELLKMDKD
                     VLSEKVKNRPQQNLYEPIRLKEGLSTKEIALIEARRSDFPSLAVDVDVSRDYLFGSTG
                     AHLIGYLGKPNEFQYSSPDFRDVPSNAFIGQWGVEKLYDRQLRGTSGERIIEMDALGR
                     ELRLIKEKSPVRGDDVKLAMDIKLQKEAEESFGEKTGALVAMKPDTGEILAMVSKPSF
                     DPNLFARGIKYRQWEELNQNKKLPLLNRSLQSQYPPGSTFKIITAIAAIEEGAIDVNT
                     KVTCTGEIVYGKWHFGCWQKHGHGTLSLHRALVESCDVYFYDLGKRLGIDKIASYARM
                     FGLGKETGLRIAKERSGMVPDTKWKQEKRKQPWYLGETFNASIGQGYVATTPFQMAQM
                     MSIVANGGYIYSPSLLMADNKPAPLTKLNIRQETFDEVKKALSGVVNENGGTGWAAKS
                     SMVNICGKTGTAQVVGMKRDSKYLPEMFRDHAWFVAFAPYEAPEIALSVMMEHGGHGG
                     AAAAPIAKRAIEVYMKGQRLENRQTTH"
     gene            9604..10725
                     /gene="rodA"
                     /locus_tag="VMU21_08510"
     CDS             9604..10725
                     /gene="rodA"
                     /locus_tag="VMU21_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HUO77608.1"
                     /translation="MKIDRRLIKNFDWVTSFLIVFLAIIGVMTIYSATRPPLPDGTMP
                     SYYIRQLYWAIMSILALLLMISFDYIWLKRASYVLYGLGMFLLLLVLLLGKTGMGAQR
                     WLNLGVISFQPSEVFKLFYIITISRYLSEMQGKIGLASLLKTFFLLAFVPIIMLIKQP
                     DLGTAIILLLVFLSLVIMKGLQRKAAALVVLISLVSVPFLGNILWEGLKDYQKNRLVA
                     FLEPETDPSGIGYHINQSKVAIGSGQVIGKGYLKGTQGPFRFLPEKHTDFIFAVFGEE
                     WGFAGNVILLFLYLSLILRGLDTASKAKDEFGSLLALGISFMFTIYFFVNVGMTLGIM
                     PVVGVPLPFMSYGGTSLLANFVAAGILINIRTRRFTLFY"
     gene            11012..11087
                     /locus_tag="VMU21_08515"
     tRNA            11012..11087
                     /locus_tag="VMU21_08515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11045..11047,aa:Ala,seq:ggc)
     gene            complement(11207..11986)
                     /locus_tag="VMU21_08520"
     CDS             complement(11207..11986)
                     /locus_tag="VMU21_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77609.1"
                     /translation="MKNTMEFRKKKILIADDSHSFLMYIGIILTRMGFTVIPAENGLE
                     ALKFLRLVEPDVVMFDVTLEKMDGIALLKHIKGHKETSHIPVVIVSADSSKESIQRCR
                     ELGCAWHISKPVKVDKIHEALEECLFSQQGMKRRHVRAPLNEKVSVICNGTLYELYTE
                     SLSEGGVYIRKKDPFPVGSEVEVVLPINGGGSLRVKGVVVYVKGLFGDVFTVPPGMAV
                     EFRGVKEHEVNTLRMYVEQLIAHDIFESQEETVIEPYESNL"
     gene            12188..12787
                     /locus_tag="VMU21_08525"
     CDS             12188..12787
                     /locus_tag="VMU21_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HUO77610.1"
                     /translation="MSPGEEKAWEALGRVDPDDVSTRTGATFDLVSGLYRFKSFGKDF
                     SVSPQDKNIFSDAPESDTLLQKLGFFFNFSVICSFYSAKDIPLTGKLIDPVNMKSGQL
                     FFRGSHVLPLDKVSERYGNDRDGFLKRGLVLGGVGLEYGDASVKLFPLPRVPVVLILW
                     LGDEEFPPRTDILFDSSAEIQLPIDVIWSIAMLSLLVML"
     gene            12973..13713
                     /locus_tag="VMU21_08530"
     CDS             12973..13713
                     /locus_tag="VMU21_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HUO77611.1"
                     /translation="MILLILVLLFSLSCSGKKEVKPDETFDPEKSIPRASDLIDNKEY
                     EDARRILLEVKNRDLTKKYAPLAQLKIADSYVKEEEYDLAVEEYKKFLEMYPDHKDAS
                     YAQYQLAMAYFVQIESPERGYGAALKALEQFEKLKRMYPRNPYREVIELRIDKCKTTM
                     ADYEFTVAEFYFNKGSYQSALGRFKQVLTKFPDYKKEPLVLYQLALSYKKLGDKDKTA
                     EYLNRLIAKYPDDKLAKQAQKELISLTK"
     gene            13661..14353
                     /locus_tag="VMU21_08535"
     CDS             13661..14353
                     /locus_tag="VMU21_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HUO77612.1"
                     /translation="MTNSPNKRKKSSSPSPSKNYYPAFLDITGKRCVVIGGGRVAERK
                     CSPLIRAGAQVVVISPKLTKGLEALKKRRLIRHIGRLYRRGDMQSAFMAIAATDSREI
                     NLKVVADAAIHNALLNVVDNPHLCNFIVPSVLRRGLLTIAISTGGVSPAMARTIRKSL
                     KELYSTELSKYLRLLNTIRTKAMQDIPDKRKRAALLKGLASPKSMRMLFQKGFREARR
                     AALISLHNITPH"
     gene            14479..15228
                     /locus_tag="VMU21_08540"
     CDS             14479..15228
                     /locus_tag="VMU21_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77613.1"
                     /translation="MKKNSIKITSKRGTEFDSEVILGQEKYLVQTEWGGAQKPHITTR
                     VYLKGQVVFTRKTDCSDMMNSSDMPSEVRECMLEQHQSAIATLKAERSVKTKTTSDYL
                     GEVKNFLRTKNKRAALRVLNEALEHYPDDPFILSYYGCLESVANKNYTHGIETCHMAL
                     ENLKKRVPFGQEFFYPVFYLNLGRAYLAAGRKDEAIEAFNRGIQIDGENNDLTWEIRK
                     LGTRRKPAVPFIKRSNPINKYIGMLLHSPRK"
     gene            15357..15794
                     /locus_tag="VMU21_08545"
     CDS             15357..15794
                     /locus_tag="VMU21_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HUO77614.1"
                     /translation="METYKQIGFKLTPQRIAILDYLNDNKGHPCADEIYRAVSEKFPT
                     MSFATVYNTLNALKRRGMLLEITIDPRKKRFDPDSEPHHHLMCIRCKKVVDIHNEFEL
                     TIPLGQRHGFEIMGNHIEFHGLCEQCRETRSEKNSQTFRGGDH"
     gene            15858..16043
                     /locus_tag="VMU21_08550"
     CDS             15858..16043
                     /locus_tag="VMU21_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HUO77615.1"
                     /translation="MSEVKTESREYVCQQCGYIYNPAKGDRKGKIPPGVPFEELPEDW
                     TCPLCGAKKARFSPMLD"
     gene            16062..16496
                     /locus_tag="VMU21_08555"
     CDS             16062..16496
                     /locus_tag="VMU21_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77616.1"
                     /translation="MAYEMRCKCGDKAASFHFKDNILSEQVIRNLYCPRCSSDIQLNP
                     GSMVVDNGWVIEYDMDVVNFMGQKIRTGTITPDSIFDEGYCTWNGLYPGDHIESVKER
                     ECIAVLAKTDPLRYLNEMKSWANERVKRLQQEGWRKAKPEEL"
     gene            16576..17067
                     /locus_tag="VMU21_08560"
     CDS             16576..17067
                     /locus_tag="VMU21_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HUO77617.1"
                     /translation="MVSKTIETAIKMETDAMKFYEEAARKTSHPFGKKMFEGFIKDEV
                     RHLQMLEDILKGLNLTTAKVNPSDGIKTVFSELKDQMMARVKASQDELSAVNIALDFE
                     SAGYEFYKKAAQDATDKKEKELLARLAEEEGEHSSILWNTYSFLRDTGDWFMWEEHGI
                     LEG"
     gene            17133..17627
                     /locus_tag="VMU21_08565"
     CDS             17133..17627
                     /locus_tag="VMU21_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HUO77618.1"
                     /translation="MSKTEQNLKDAFAGESQANRKYLAFAKKAEAEGFTQVARLFRAA
                     AEAETVHAHNHLKELGGIKSTKENLQEAINGESYEFQKMYPQMIKDAESEGNKGALRS
                     FNYANDVEKIHAALYKKALENLGKSPDADMYVCQVCGYTVEGEAPDECPVCKAKKQSF
                     KKID"
     gene            17935..18669
                     /locus_tag="VMU21_08570"
     CDS             17935..18669
                     /locus_tag="VMU21_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77619.1"
                     /translation="MGLAIFSAFPHEIRPLVKNLRAARISKTLPFTLFLAPYASGEVI
                     VVLTGMGTPNAEAALDYVIKEHSPTIILSSGFGGALYADAAIGELIWASRVLLVNDRV
                     RDALDLPDRDHLYDRLSQKVTIQEGSIVTLERWMKKPEIKRALSKSLLFPVCDMETFP
                     LAKATITRGLPFVAIRSITDRSHEEIPPELLCVADKSGNYRLSRSLKVLIGNPSLIPR
                     AIDLGRNSAIASKNLWRAVRALIEVL"
     gene            complement(18676..19533)
                     /locus_tag="VMU21_08575"
     CDS             complement(18676..19533)
                     /locus_tag="VMU21_08575"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HUO77620.1"
                     /translation="MEFFPLLFHTVVLRPYVFVFLGIYLFGCSMHLGLKRALLFFVAG
                     YLIAWVSEYSSIHNGIPYGYYYYIEHTKGLELWVLGVPFMDSLSYIFLAYAGYSMALM
                     VIAPVLYSGRRIYLLETKKVRNSLYARFLGTLFFVYLDIIIDPVALQGHKWFLGQVYG
                     YPEKGVYFGVPISNFIGWFLVGFLMIYVLQKIDRFLDTCTDYAGYKLPWRYLIGPGLY
                     FGILIFNLSVTFAIEEYHMGWVGVFIVLLPIVLGYHILTAKRSSEEIRKAIELHLQDF
                     PLSAVPKMS"
     gene            complement(19533..20537)
                     /gene="hpnH"
                     /locus_tag="VMU21_08580"
     CDS             complement(19533..20537)
                     /gene="hpnH"
                     /locus_tag="VMU21_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HUO77621.1"
                     /translation="MRFPISLYSSLAIAAFRHLIKGKKRFPLVLMLEPTHRCNLACAG
                     CDRIRLQSKEQSQDLSLETCIRAVVESGAPVVTVTGGEPLLYPDLKPLVAELLRMKRY
                     VYLCTNGLLTDSFVDEAYPHPRLTLNFHLDGLEETHDQITCRPGTFKKTIETIKRAKE
                     KGFRVSTNTSVYKTTDTGELERLFELLKSIHVDGILISPAFSYESVEGSIFLSRDEIR
                     EKFGKIASFFNRFPFISSPLYVDFLKGRREMRCVPWGNPTRNPLGWKSPCYLITDRYY
                     DTFDELMEKTPWEKYGTGNDPRCRNCMVHSGYEASVMKTAFLHPGDLIRLLLWNLKKS
                     "
     gene            complement(20738..22639)
                     /gene="shc"
                     /locus_tag="VMU21_08585"
     CDS             complement(20738..22639)
                     /gene="shc"
                     /locus_tag="VMU21_08585"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HUO77622.1"
                     /translation="MLLDRVTEAIGKVHSYYMTQQHEDGYWWYELESNVTMTSEYLML
                     LHFLGLRDEERDRKIVNHILKHQRADGTWSLYWEGKGDLSTSIEAYFALRIAGLSPDD
                     EPLKKARAFILNEGGVEASRVFTKIFLALFGEFDWKAVPSIPVQMNLFPPWFPINIYN
                     FSSWARTTVVPLSVIAEYKPVRPLNDFAGIKELYKEPHKMPPLTPKKLPPFSWKRFFL
                     IQDKLLKTAEALPIRPMREKALKRAEQWILEHQEPCGDWGGIQPPMINSIIALMAMGY
                     EVSSEPIQKGLEALKRFCIENEHELSLQACISPVWDTALTGLALLYSGKERSHPSLIR
                     ACRWLASKQIFTKGDWSMKRPSLNPGGWAFEFENSWYPDVDDTAVVLLLLNRYTDRDF
                     VKEENMESGLQWILGMQGKDGGWGAFDVDNDMKLLNQLPFGDLEAMIDPSTPDLTGRV
                     LELLGLLGYEYTNDTVQRAIAFLKRTQEKDGSWWGRWGVNYLYGTWSVLMGLCAIKED
                     MSSPYVRRAVMCLKNNQNSDGGWGERCESYEDRTCGCRGKSTPSQTAWAILALIAAGE
                     DGCEEVKKGIVYLLERQKPEGTWDESEFTGTGFPRHFYLRYHNYRNCFPLMALGQFCA
                     RLQKSGSQP"
     gene            complement(23039..25684)
                     /locus_tag="VMU21_08590"
     CDS             complement(23039..25684)
                     /locus_tag="VMU21_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HUO77623.1"
                     /translation="MMGRAKDYPARLLVWWVTSVQRHASLVMVITLLITGGIFSYSLT
                     HFNINVDTTSMLSEKLHFRKLEKDFSGVFPKLRDTIVIVLDADTEERARSARTQLAER
                     LRKETGLFKSVYEPGGGSFFEKNGLLYLSVKELDDFADTLASAQPFLALLSQDLSVQG
                     LFSMLTMALGHGEEISLKDLRIKLLFDGMSEAFESVTNNRKYHLSWQGMMFGKELPKQ
                     RHQFIILQPYLDFTKLSSGEAPLETVRRVLKELGFHGEHGVTARITGDVALSHENLTE
                     VRNSVGVATLVSLFLVGCSLYIGLGRSGRLVFASLTTLIIGLIWTTGFAVAFIGSLNL
                     ISITFAVLFIGLGIDYSIQFCLRYRELVVSGFDHGECIGMTAKGVGRSLLVSCIAIAI
                     GFFSFVPTAYAGVAELGLISGMGMFISFFANLTILPALLTLLPLQRRDMPKSSLGGMI
                     LTLPYKYPKAIRSVAIILGLGALFLLPKVYFDYNPLNLYDKKSEAIATVKDLFKDPNS
                     SPWTISILVKDAGEAKALAEKLGNLKEVKMAITLSDFVPEAQPEKLRILSDIRLMMPP
                     TLKNLQVKHPSYEQKLTSLNKLEGALKKSLGSLSYEDASSARRLYEDIGRFQTMLKNS
                     ENGQKVFAALEESLLSSLPSLLERLENLLQASAVDMRSLPQDLVTQYVSADGRYRVQV
                     FPRENILDRNALERFIHSVRTLAPDATDTPVTVYESGRAVVSSFRQAALSALVVITLY
                     LLIELRSISFTITILVPLLLAMLLTAVSSALLDIPLNFANVIIVPLLLGVGVHSGLIF
                     VLRYRTEPPPDGNMLKTSTARAVLFSTLTTIVSTSSLSFSAHRGIASMGMLLVLCLSF
                     MLVSTLILLPALLELLGRRNHTKGN"
     gene            25826..26470
                     /locus_tag="VMU21_08595"
     CDS             25826..26470
                     /locus_tag="VMU21_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO77624.1"
                     /translation="MNVAKQEKSMRMAHWLIVSGIFLYTVLFPLPGKAASAGPAEVIK
                     TFNAALLESMKRADELGYSGRFKLLDPVIRNSFALSYMAGQSLGRYGKTIKKTEQSAL
                     VKTYTEWSIATYASRFDGYSGERFEVVSESPSDQGTVTVISKIIQPDKEEVAFHYKLR
                     KFGGNWRIVDIQISGVSQLALTRSQFTNVIKEKGFDGLIALLKTKIEGLAQGKP"
     gene            26783..27589
                     /locus_tag="VMU21_08600"
     CDS             26783..27589
                     /locus_tag="VMU21_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO77625.1"
                     /translation="MESIMKGSSFGLLHFLGGRSLRIINNLGAVVIFFALSFLKIFRP
                     KQIREIIQQVFYIGARSSTIVMLIGLFTGMVLGLQLYYTLIKFGSTGVLGSVVALSLI
                     RELGPVLTAIMITARAGSAMTAELGILRISEQIDALYTMRIDPLRYLISPRIAASIIS
                     FPLLTAFFDLIGILGGYLTGVVLLDVDKGAYFYRIHSYVELVDIRGGFIKSLVFAVLV
                     STICCYQGYFTHLRTDSYGAKSVSLSTTSGVVFSCVMILISDYVVTSFLM"
     gene            27598..28845
                     /locus_tag="VMU21_08605"
     CDS             27598..28845
                     /locus_tag="VMU21_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO77626.1"
                     /translation="MDTPLIEFREVTKRFNGKTVLDRMNLMIYENQISTIIGKSGTGK
                     SVLLKHIIGLLTPDEGIILFRGKSIHAMKKSERDSYRSKISYMFQNNALFDSMTVFDN
                     IALPLRQTTDLGKKDIEQKVTTRIEQMELSEAVTKFPSELSGGMQKRVALSRALVTDP
                     TIVLFDEPTTGLDPIRKNMILSMIAHYRKMFGFTAILISHDIPDVFFVSDRILILWEG
                     KIVFQGTYEESTRLNHPIIDEFLKSLEGVQDELTGLLSKEMFKARYAITLGRGRVDTS
                     LTAVLFSVHFDLLVNALGPQAAIEAIKKLGEYVNEYFGPVGGFSARHRRGEVLTMLPH
                     IDIEEAMQLVDNFAKGLEQKVLPEIQGLTTAKIGAGECFELFVTAGIIEGTSADDIDQ
                     LIEKAEASQKVIARYQCVKEVSV"
     gene            28845..29318
                     /locus_tag="VMU21_08610"
     CDS             28845..29318
                     /locus_tag="VMU21_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HUO77627.1"
                     /translation="MRKYSAETVVGIFVVFGLLCVAYMTVKLGRVSLWGDDYNTFYAR
                     FSSVSGLRVGSPIEIYGIEVGRVTRLTIDPEKQVALVELKIGKGFKVYDDATATIKTA
                     GLIGDKLVKIDPGGGGDILKPGGTITETTSPTDIEDLISKYAFGDVKKEDKKETK"
     gene            29336..29929
                     /locus_tag="VMU21_08615"
     CDS             29336..29929
                     /locus_tag="VMU21_08615"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUO77628.1"
                     /translation="MKKSLIGIALFLCVIIPLPVFAGVPLDTVKTHVDKVLEVLRDPS
                     LKAESSKKLKKDKICAIAEKMFDFTELSKRTLAQHWSKFTPEQQKEFTSLYKSLLENT
                     YADKILLYTDEKILFTKEVPLTEKTAEVQTMVVRSTQETPIYYRVILKDGTWMVYDVI
                     VEGISIINNYRSQFREILVNNPPEYLLETLRKKVGKA"
     gene            29965..30885
                     /locus_tag="VMU21_08620"
     CDS             29965..30885
                     /locus_tag="VMU21_08620"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HUO77629.1"
                     /translation="MKHICRFVLTVMFVTYPVILWAQAFDEPDAAVHTSPGVVHSDAG
                     LMGFPQPLSLSDIDGQRETPAIIVAQAIAGEEIPARELATHAGKEAPEEGEEEVPLIA
                     DPIAPFNKAMYHVNDKLYFWLLKPVTQVYSHTVPEPFRVVFNNAFDNLWAPSRIVNNL
                     LQLRFKAAGNEFIRFIFNTIAGIGGLGDVAAPALGIKKQEADFGQTLGHYGIGHGFYL
                     VLPVFGPSSVRDGVGLAADRFMYPLSYISTSDLTFGEAAGIYAGEKVNDTSFHIGEYE
                     SFKDSAIDPYVGMRDAFVQYRQKKVEDSKK"
     gene            complement(30909..30984)
                     /locus_tag="VMU21_08625"
     tRNA            complement(30909..30984)
                     /locus_tag="VMU21_08625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30949..30951),aa:Lys,seq:ctt)
     gene            complement(31106..31181)
                     /locus_tag="VMU21_08630"
     tRNA            complement(31106..31181)
                     /locus_tag="VMU21_08630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31146..31148),aa:Arg,seq:ccg)
     gene            complement(31304..31379)
                     /locus_tag="VMU21_08635"
     tRNA            complement(31304..31379)
                     /locus_tag="VMU21_08635"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31344..31346),aa:His,seq:gtg)
BASE COUNT         8353 a         7684 c         7691 g         7889 t
ORIGIN      
        1 tatgtgggag aaggtgggaa tcatacgatg tgcggaatcg ctctccgtgg cgggagatat
       61 catttcccga tggcttccct cagttggagg agcttccatc aagagagaag aactggaact
      121 caagaacatg attaccgttg ccagtctcat tactgaagcg gcgatggcac gaagggggag
      181 cgttggagcg cactaccgct cggactatcc cgccaggggt gagagctggc aacagcatcg
      241 cacatggcaa aagcctgagt aaagaagatg tacggggact acggcttcac ttcccctttt
      301 cataataatg cataattcct tcatatccat ctggttcaat ggaatcaaag gattctttat
      361 gataatgcaa agactgggaa tcgaggggtt tcccagcaaa aaaaggaggc atagaggctg
      421 aaaaagagca gacgaaagaa cctaatatga ttatgataag gtgaggacag gcacgagggt
      481 aatcagcatc gcaaagtggg ccaggaatgc atattcccct gtctcatgca tcatatcatg
      541 attttggagc ggtatcttct ccagcctgaa tatgatatgc ctttatcttc cggtgcaggt
      601 tactcctttc gatgtccagt atctcggcag ttcttgatat attccagtta ttctcttcga
      661 gttttctcaa gatgaagtcc ctttcaaagg cttcccgcgc ttgcttcagc gttccgtaac
      721 tgaaaaaatc ggcaccctca tgtcttccgg cgaggcggaa gtcccttgcg tggatatctg
      781 cagaaggggt catgatcgca agcctttcga tgaggttctt tagttccctt atgtttccgg
      841 gccagttata ttcctgaaga ctctttattg cgtcagccat aatcctcttt ggtggttgtc
      901 cgtactctgt tgcgagagac tttatgaaat gatccacgag ggccgggata tcctccttat
      961 gttcccttag ggggggtacc acaatgggga ctacattaag cctgaagaag agatcatccc
     1021 taaaattccc tctcttcacc tcctccgtga ggtccttgtt tgtcgcagca ataatcctca
     1081 catcaacctt gatagtcgta ttcccaccaa ccctctggaa ttcctgcgtc tcgatcaccc
     1141 tcagcacctt tgactgggtc tggagggaca tatccccgat ttcatccaga aaaagagttc
     1201 ctctgtcagc caactcaaat tttcccttct ttttttcaaa ggcacctgta aacgaaccct
     1261 tttcgtgacc aaatagctca ctctcgataa gttcataagg catcgccgca caattaagct
     1321 ccacaaaagg cctttcgcct ctagtgctct tagcgtggag aagccttgca acaagctcct
     1381 tccctgaccc gctctcaccg gtgatcagca ctctactgtt actctgtgca gccatctcta
     1441 tctgggcatg gagggtcttc atcggagaag agtcaccaat aagcctccat ttccttgtga
     1501 ggttctcctt gagatccctg ttttccctct cgagggcgct cctttccaaa gcacgtctgg
     1561 cactgaggac aatcctttcc agggaaagtg gtttctcaag gaagtcgtac gcccccattc
     1621 tagttgcctt caccgcaacc tcaatatttc cgtgtccgga aatcattatc acgggtgttc
     1681 ctctcttgag ggccgcgatc tcctgcagcg tctgtatgcc gtccattccg ggtaaccata
     1741 catcgaggat tattagatca ggaaactgct cctgcagaat tacaagggca tcttctccgg
     1801 aaccggcggt agcgacacga tacctctcat cctccaaaat cgccgaaacg ctctccctga
     1861 tgccttcctc gtcatcaacg ataagaacta aaggatccaa tctacccctc ctttatcggc
     1921 atctcaatgg tgaagaccgt tccttgaggc tcgttatccc tcaccctcag atgccccata
     1981 tgctccctga taatcttgtt cgcaatggca aggccgagcc ctgtcccttc cttttttgtg
     2041 gagaagtagg gaagaaaaag cttttcttta tcttcttccc gtattcccgg gccgttatca
     2101 gccacgtcca taaacaccct gtttgaagat acattaagat gaactgtcac ctctatatta
     2161 cccccgctgt gcatcgcctg tatggcgttc tcaaaaaggt ttataagaac tctcttgaac
     2221 tgttctctgt ccatatcgat aggtggcata tcctcaggaa gcgacaccct tatggaaaag
     2281 cccttgtagt ccctgtaaag attcactacc tcatttatta taccagcgag gatggtggac
     2341 gatttatgaa tttccggcat tttctcgaag cgagaaaact cgtccactag gtttctcagg
     2401 ctgtcaacct ccttgatgat ggttctcatg gatctctcaa acacctgtgg aaagtcttca
     2461 tcttttcgtt cccatttttt cagcatccgt tctgtggaga gctttatggg ggtgagggga
     2521 ttcttcacct catgggcaat ccttctggcc acttcttgcc acgcgagcgc tttctgtgcc
     2581 tttatcacat tggtaaggtc gtcaaagaca acgagaagac ccagatattc cgaggcgtta
     2641 tttctcaatc ccatgattga gaccctcagc atggtacgct ttcccttaat ggtagcccat
     2701 atctcctttt ccaccatacc cagcgtcctt aattttatcc cgcgaatcat ctcctgaagg
     2761 gaatcggaac ggagtaagga taggagttct ttgtagtttt tccccatcac attctctaac
     2821 gcaacattga ggatcgcgca ggcggcagta ttcatggcga ggatagtgcc gtcagcatcg
     2881 agcgagataa ccccactttg tatgttctcc aagatgttct ccataaatag ccttcttctg
     2941 tcagactcta cgtaggcttt ctggagagat gccttaccct ccttaagttc tttcaccata
     3001 tgattaaacg aattaatgag aagccctatt tcgtcgtctc tcttcagggc aatatccacg
     3061 tccagattcc ctgacgcaat ctctcctgtt gcttcggcaa gcatctggat cggttccgag
     3121 attcccctgg cgaccctgag ggagacccag agtgcggtga agataacgat aagcgtgaag
     3181 aatcctaaaa agagttgata gttaagtttc aggggctgtt tccaagcctc aagttgtata
     3241 tagtcttcat aggcgcgctt gatcttctct acattgcggg taatgtccct cggaatgtgg
     3301 gattccacaa tcacaactcc tgaacgcctt cctcctgtgc tatcaggaac tactgccctt
     3361 acggtgtctc cgttctctcc tgaaatgacc tctgtccctt cctctcctcc aaaggcttct
     3421 gtgaccacat cagagggatt atcgggaagt tcggaaaggt gcgtgaccgt atattccatg
     3481 ggtatagtac cctttgacac aatggagcgt gccgcttcaa gggtcttttt gcgctccgta
     3541 tcataaaggg ctcttgcgat gactaacgta ctgtcaatgg gctgtctgaa ctgtggggtg
     3601 aaccacctgt caatatagtt tgttacgaga ccgctggcac ccaaaaaaag aagcacggac
     3661 ggtatggagg tgaggattac aaacaccacc acaatctttg tcttgaaccg ggagcccagg
     3721 attttccgct tcttttccaa ataaagcttg gtgagcgcct ttgccacaaa gaacatcagg
     3781 gtgaggagcg ctataatgtt cacattgagc agtatgagga gggtaaccct cgtggtgatt
     3841 gagagggaag cctgacgcat gagatgcagt tccacagtgg aaacgagaat cacaaaaaga
     3901 agaagccccc ccagtatcat gccgagtttt ttcatttctc ggtccctgtg ctgagtgagg
     3961 gagactcctt ggagagctta aagtccttct cagaaacaaa aaagaggaga taccctatta
     4021 ccggcgggag ctcccttagc cgtgattcca cggtaacctt cacatagtag tccgatgact
     4081 ggagttcttt tatgttgatg agtttgagat cctttatggt taatgtccaa tccagcatgg
     4141 agtcaaagcg cttgaatctt ttttcggtaa gggtagcacc atcaaaggtc gtcgcagtgt
     4201 attccttttt gatgggatca gaccgtaagg tcttgacgat tttcttcccg gcgatgaact
     4261 cattaggcca catgcgccag accctaaaga gatctatata aaaggtgatt tcctttgata
     4321 taccattttt cagatcatcg acattctttt catcaagtgc caatcctgta cttaccacaa
     4381 tctcatcatt cgtcacccgg gcatcgggtc ctatgatctc tgcaccagag gcattcagaa
     4441 gcatgagaaa gagggataaa aaggccagag aaagaataat cctcgccatt taagacttga
     4501 catctccgga aaaatttatt ttcattatac cataatgaaa aagggagaaa gtatttagca
     4561 gcagccagtt agagaacaaa gaaaggagaa ggtttatatg gaactcgagg caataaggat
     4621 agagataccc gaagaatgca atatcatcct cggccagacc cactttataa aaactgctga
     4681 ggacctctac gagattgttg ccaccaccgt gccccaggcc aaattcggga tcgctttcac
     4741 cgaggcttcc ggtccgtgcc tgataaggac agagggtaac gatggaatgc ttattaactg
     4801 ttgtgtggcc atgttgaagg acataggggc cggccatgtt ttctgtgttc tattaaagga
     4861 cgcatatccc attaatgtac tcactcagat aaagagttgc caggaggtat gcagagtcta
     4921 ttgtgccact tctaatccgc ttgaagttct cgtcgcatcc acaaagcagg gaaaagggat
     4981 tataggggtg gtggatggct ttcccccaag gggtgtagag acctctgacg acaggctcag
     5041 acggagggag ttcctgagga cgatcaggta taaggcatag gccgtacctg atatgcacac
     5101 tcaaggggac ctatcagggt gcaattattg aaaaaaacag ccgtatttgt taatattatg
     5161 ttctatgtct gatgaggcga agagcgccag cctgcttctc agggtgtttg cgaaaattct
     5221 tgatttcatc ctcatcgctg ccatggctga ggtcatacca aaggctggtt tttatgcagg
     5281 gttatcctat ctcctcatca gtgatggtct cttcgaagga aggagcattg ggaagttgct
     5341 gatcggcctg cgagtaatct caacggctca tgacggacca tgctcgatga gagagtctat
     5401 cgtaaggaat gcccctctgg gtgcgggact cttatttttt aagttgccct gggtcggctg
     5461 gattgttatc gcgggtgtgt ctgcggtgga gttcttgata ctcctgggga gcaaagacgg
     5521 gaggcgccta ggagatgaat tggcaaagac cacggtagtt gaactcgtta aaagcaaaga
     5581 cagtcaggag gtataatgct ttttcataaa gtggtgggaa tgttctctaa tgacctggca
     5641 gtggatctgg gcaccgcaaa tacccttgtc tttgtcaagg gaaagggcat tgtctgtaat
     5701 gagccttccg tggttgtggt gaggaaggaa gataagaaaa ccatcgcggt tggtgccgat
     5761 gcaaaaagga tgctgggcaa aacaccttct aatattattg ccatacgacc gatgaaagac
     5821 ggcgttattg cggactttga cgccacgggg gagatgctga aatacttcat caagaaggtc
     5881 cacaacagaa agagctttct ctctccacgg gtaatcatcg gggtaccctc tggtattacg
     5941 caggtggagc aaagggcggt gaaggacgct gcgcaggcat ctggggcacg ggaggtatat
     6001 ctcattgaag agcccatggc cgccgccctc ggcgtaggtc ttcctgtggg agagccctcg
     6061 ggcaacatga tcgtcgacgt agggggaggg accacagaca tcgcggtcat ttcccttgat
     6121 ggcattgtgt atagcaaggc ggtcagggtt ggcggtgata agatggatga cgcattaatt
     6181 gcctacataa aacggaagta taatctgctc ataggtgaca gaaccgcgga gctgataaag
     6241 atcgagatcg gctcggcatg cgtagtagac cccggcgtca caaggactat ggagataaag
     6301 gggagagacc ttatttcggg catccccaaa atcatcctgg taacagaaac cgaaatacga
     6361 gaggccctgc atgaaccggt gagtgtcatc cttgatacaa tcaagataac cttggaaaat
     6421 actccccccg aacttgctgc ggacattgtg gacaggggta ttgtattggc aggaggcggt
     6481 gctttgctga gggggcttga catcctcata agggaggaga ccggacttcc cgtcattgtc
     6541 cctgacgacc ctttgaccgc agtggcaagg ggcgtaggaa aaatgcttga cgaactagaa
     6601 ctcctgaaga aaattgccat aacctaatgt ccattaggag acttttcccc cttttcctgc
     6661 tcgtggtgct ttccttaacc cttatgacct accagagcaa taaaggaccc attgcaccct
     6721 tcaggtttct gagcaatccc cttaactacc tgaatgacat gatccattca atatcctctt
     6781 cgttgaaaga gcctttcaga aaaatcatgc taagggatga agacatgaag aagctcaaag
     6841 gacagataga gagtcttctc cttgagcaac agagataccg tgacctcttc ctcgaaaacc
     6901 agaggctgag agagatcctt tccgtaaagg agagggaaag aaggtatgtc gccactgcga
     6961 ggattatttc aaggggaccg gacctatggt caaatatgct cgtcatcgga aaggggacgc
     7021 gagaaggtat tgcaaaagat atggcggtga tcactcccct cggccttgtg gggaagatct
     7081 ctgtagtgac tgatcattac gcgtatgtac tcctcgtgac cgatatcagt ttttccgctg
     7141 ccgtgagaat ccaggacacc agggaggagg ccatactctc aggaacgggt acgaggcgct
     7201 gcgttctgca atacattcct gaagaggctt tgatccggga aggagagatg gtcgtcacca
     7261 ccgggtttga tgacgtgttt ccccaggagc tcatcgtggg ctatgtgtcc agagtgtcca
     7321 aaaaggggtc cagcatattt cagcaggtag aggttacccc atctcaggac ctcacgaaac
     7381 ttgatgaggt gataatcgtc aggcgatgaa atcgttcatt attttccttg gtgtggcact
     7441 tgcctttctc cttcagacaa agatctcctt cctcggggtt tcccctgatc tcaccgcagc
     7501 ggtggcgtat tattttggga taaccggagg ttcaacgaag ggggttctct tcggctccct
     7561 catcggggtc atcgaggaca gcgtgggagg aaacatcctg ggtccgaacc ttctgggcaa
     7621 aggcatggta ggcttttttt catccttcat gtccggcagc ctgttcagat ggactccttt
     7681 tctgggagtt gtatcccttg ttattcttac cctcacagat ggtagtatta ttctgttatc
     7741 aagggcaata tttgatgcaa tgcccacgtc tatgtccagc gggattttta cgctctttat
     7801 gcaagggctc ttcaatggca tcataggtat atttataagg ccaaggaatg tcgattaacg
     7861 gaaattcatc agaaaaggtc ctcatctcag cgtatttaat cattgcctta tttttcatcc
     7921 tgaccatacg gatttggcag ttgcagattc ttcaagggag tgaatacagg aaattgtcgg
     7981 aggaaaacag actgcgtatt gtgagggttg cggcaccgcg tggaataatc tttgacagaa
     8041 acggcattcc tcttgtgaag aacaccccat actattttgt gtctatcagt ccccagaatc
     8101 ttgacggtct tgacatctcc gcccttgcgg agcttttgaa gatggacaag gatgtcctct
     8161 cggaaaaggt gaaaaatcgc ccccagcaga atttgtatga acccatacgg ctcaaggaag
     8221 gcctctccac gaaagagatc gccctcatag aggccaggcg ttcggatttt cccagcttgg
     8281 cagtggatgt ggatgtgagc agggattatc tctttggaag caccggggcg catcttatcg
     8341 gataccttgg caaaccaaac gaatttcaat atagcagtcc cgatttcagg gacgtccctt
     8401 ccaatgcatt catcggtcag tggggagtcg aaaagctcta tgacaggcag ctgcggggaa
     8461 cctccgggga aaggataata gagatggatg ctctggggag agagttgaga ctcattaagg
     8521 aaaaatcccc tgtgcgggga gacgatgtca agctggcaat ggatattaaa cttcagaaag
     8581 aggccgaaga gtccttcggg gaaaagacag gagcccttgt cgccatgaaa cctgatacag
     8641 gggagatact cgccatggta agcaaacctt cttttgaccc taacttgttt gcgaggggaa
     8701 taaaatacag gcaatgggaa gaactgaatc agaacaagaa actccccctg ctcaacagat
     8761 cccttcagag tcagtatccg cccggctcca cattcaagat cattaccgct attgcggcca
     8821 tagaagaggg agctattgac gtcaatacaa aggttacatg taccggggag atcgtgtacg
     8881 gcaagtggca ttttgggtgc tggcaaaagc atggacacgg aaccttgtca ttgcaccgtg
     8941 ctctggtgga gtcgtgtgat gtctactttt atgatttagg caagagactg gggatagaca
     9001 agattgcctc ttatgcccgg atgttcggac ttggcaagga aacgggcctc cggatagcga
     9061 aagagcgaag cgggatggtc cccgacacaa aatggaagca ggagaagagg aaacagccat
     9121 ggtatctggg tgaaacgttc aatgcatcca tcgggcaggg gtatgtggcc acaacccctt
     9181 tccagatggc tcagatgatg agtatcgttg caaacggagg atatatctat agcccctcac
     9241 tcctcatggc agacaacaag cccgcgcctc tcacaaaact aaacatcagg caggagacct
     9301 tcgacgaggt caaaaaagct ctctcagggg tggtgaatga gaatggggga accggatggg
     9361 cggcaaagtc atccatggtg aatatctgcg gtaagacagg gacagcgcag gtagtgggaa
     9421 tgaaaaggga ttcaaaatat ctccccgaga tgttcaggga tcatgcatgg tttgttgcat
     9481 ttgcgcccta tgaagcaccc gaaatcgccc tttccgtgat gatggaacat gggggtcacg
     9541 gaggggctgc agcagctcct atcgcaaaaa gggcgataga ggtatacatg aagggacaga
     9601 gacttgaaaa tcgacagacg actcattaag aactttgatt gggtaacgtc tttcctcatt
     9661 gtcttcctcg ctatcatagg ggtaatgact atttacagtg ccacacgtcc ccctctcccc
     9721 gacggcacaa tgccgtctta ctacataagg cagctttact gggcgatcat gagcatcctt
     9781 gctctcttgc tcatgataag ttttgattat atctggctaa agagggcttc ttatgtgctc
     9841 tatggcctcg ggatgttcct gttacttttg gtcctcctcc tcgggaagac cggaatgggc
     9901 gcgcaacgct ggcttaatct cggggtcatt tcttttcagc cctctgaagt attcaaatta
     9961 ttctatatca ttaccatatc ccgatacctc agtgagatgc agggcaagat agggcttgcg
    10021 tcattgctca aaacattttt tttgctcgca ttcgttccga taatcatgct gataaaacag
    10081 cctgaccttg gcaccgccat tattctgcta ctcgttttcc tttccctcgt cataatgaag
    10141 gggcttcaga gaaaagcggc cgcactagtg gttctgatca gcctggtctc tgttcctttt
    10201 cttggcaaca tcctctggga gggcctcaag gactaccaga aaaacaggct tgtcgctttt
    10261 ctggagcctg agaccgatcc ttcgggcatc ggctatcaca ttaaccaatc aaaggtggcc
    10321 atagggtcag gccaggtcat aggaaagggc tacttgaaag gtacccaggg accttttcga
    10381 tttctccctg aaaagcatac cgatttcata tttgcagtct ttggggagga atggggcttc
    10441 gcaggaaacg ttattctcct tttcctctat ctttccttga tcctccgtgg ccttgacacc
    10501 gcaagcaaag ccaaggatga atttggaagc ctcttagccc tgggaatatc ctttatgttc
    10561 accatatact tttttgtcaa tgtgggaatg accctcggca ttatgcctgt cgtaggcgta
    10621 ccgcttcctt tcatgagtta tggcggcacg tcactcctcg caaatttcgt cgcggcagga
    10681 atacttatca acatacggac aaggcgattc acgttgttct actagtttct cgtgacagaa
    10741 attgattata tgagaaagcc aggcttgcca atgagtacaa aagttcttta gaaatagtta
    10801 cttacaagtt actaacacct gtgaataact aaattcccgc atttgggagg cgtgggaaaa
    10861 aagattgagg tgttcagcat tgacgccctc aaaacaaacc ctcaatctca aaactgagtt
    10921 gacaagggga gcgtaaaatg tggtatttta tcaggacttg ggctactatc tgtcattcac
    10981 agagttgcat agtatgtcca tgattcttaa aggggctgta gctcagttgg gagagcgctt
    11041 gaatggcatt caagaggtcg tcggttcgat cccgatcagc tccaccatta aatattgctg
    11101 cataggcaca tcacttctca ccatcatttc aattagggat attccccttt agggtgatat
    11161 cgggacttcc tgtttctata atgttgaggt gagaaaacgc ggtaacctaa agattgctct
    11221 catacggttc aataaccgtt tcttcctgac tctcgaaaat gtcatgggcg attaattgtt
    11281 ccacatacat tcttaatgta ttgacctcat gctctttgac tcctctgaat tccactgcca
    11341 taccaggtgg gactgtgaaa acatcaccga aaagcccttt cacatagacc acgacccctt
    11401 tcactcgtaa ggacccgcca ccattgatcg gcaaaaccac ttctacttca gaacctacag
    11461 ggaacgggtc ctttttcctg atatagacgc caccctcaga caggctctct gtataaagct
    11521 catagagggt tccgttacaa atgacactga ctttttcatt caacggagct ctcacatgcc
    11581 tccttttcat tccctgctga gaaaacaaac attcttccaa cgcctcgtgg atcttatcca
    11641 cctttacggg ctttgatata tgccacgcgc atcctaactc cctgcatctt tgtattgact
    11701 ctttgcttga atcagcagat acgataacaa ccgggatatg ggaggtttct ttatgtccct
    11761 taatatgctt cagcagcgca atgccgtcca tcttttctaa ggttacgtcg aacatcacta
    11821 catccggttc tacaagcctg agaaatttca gcgcttccag gccattttca gcagggataa
    11881 ccgtaaaacc cattctcgtc agaataattc ctatatacat caggaatgag tgactgtcgt
    11941 ctgctatgag tatttttttc tttctgaact ccatcgtatt cttcatcggt tttttcccta
    12001 tcgctcaccg tgcattgcaa tggcgggatg aaaggacagc gtcgttgcga gattcccagt
    12061 gcgtggtagt tgcattaagt ttcctttacc aataccagcg cgaaaagttt ccgcacttcg
    12121 ctggtcatcc cccgcccgcc aaaagaagtg gagagatcgc tgcaggagaa ttagtctata
    12181 ataccttatg tcacctggag aagaaaaggc ctgggaggca ctgggaagag ttgatcctga
    12241 tgatgtgagc acgaggacag gggcaacgtt tgaccttgta tcaggtcttt acagattcaa
    12301 atccttcgga aaggatttct ccgtctcacc ccaggacaaa aatatattca gcgatgctcc
    12361 tgaaagtgac acccttctcc aaaaactcgg attttttttc aatttctctg tcatctgctc
    12421 tttttacagc gcgaaggata ttcctttgac aggaaaactc atagaccccg tcaacatgaa
    12481 aagcggtcaa ctctttttcc gcgggtctca tgtccttccc ctggataagg tgagtgaacg
    12541 gtatggcaat gaccgggatg ggtttcttaa gagaggactg gtgctgggcg gcgttgggct
    12601 ggagtatggc gatgcatcgg tgaagctctt tcctctccca agggttcctg tggtgttgat
    12661 actctggctg ggagatgaag aattccctcc gcgcacagat attctgttcg attcctccgc
    12721 tgagattcag ttgcccatag atgtcatatg gtcaattgca atgctcagtc tgctggtcat
    12781 gctttagaaa aggaaccgtt gcccgctgtt cgacctcgtc aacagggaaa cggtgtcaga
    12841 acttcttctc gtgcccatat cactcccctt gacataaaac ttctctgacg gctttaatat
    12901 catatgccat tgcatttttc caagagatac ttacaggaaa acccttggtt ttctttcagg
    12961 gcgggggccc tcgtgattct tcttatcctt gtccttctct tttctctctc ctgctccggg
    13021 aaaaaggagg ttaaaccgga tgaaaccttt gatccagaga agtctattcc acgggcgagc
    13081 gacctcatag ataataagga atatgaagat gcgagaagaa tacttctcga ggtaaagaac
    13141 agggatctca ccaagaaata tgctcccctt gctcagctga aaatcgcaga ctcttatgta
    13201 aaagaggaag agtatgatct cgcggtcgag gagtacaaga aatttcttga aatgtatcca
    13261 gaccataagg atgcatccta tgcccagtac cagctcgcca tggcctattt tgttcagata
    13321 gaaagccctg agaggggata cggtgcagcg ttgaaggccc tggagcagtt tgaaaagctc
    13381 aaacggatgt atcccagaaa cccttaccgg gaagtgattg aactccgaat cgataaatgt
    13441 aaaactacca tggcggatta tgaatttact gtggccgaat tttactttaa taagggctct
    13501 tatcagtccg cgctgggaag gtttaagcag gttctcacaa agtttcctga ttacaaaaag
    13561 gaaccactgg ttctctacca gttggcgctg agctacaaaa agcttgggga caaggataag
    13621 acagcagaat acctgaaccg cctcattgca aaatacccgg atgacaaact cgccaaacaa
    13681 gcgcaaaaag agctcatctc cctcaccaag taagaactac tatccggcat tcctggacat
    13741 caccggaaaa agatgtgtgg tcataggggg aggaagggtc gcggaaagga agtgctcacc
    13801 ccttatcagg gcgggtgcac aggtagtggt aataagcccc aaactcacta agggacttga
    13861 agccttaaaa aaaagacgcc tgataaggca cataggtcgc ctctatagaa gaggagacat
    13921 gcagtctgca ttcatggcca tcgctgccac agattcaagg gagataaact tgaaggtggt
    13981 tgcggatgca gcaattcaca atgcccttct gaacgttgtg gataatcctc acctgtgcaa
    14041 ttttatcgtt ccttcggtcc tgcgtcgcgg tcttttaacc atcgcaattt ccacaggtgg
    14101 tgtgagccca gcaatggcaa gaacgatacg gaaaagcctt aaagagctct acagcaccga
    14161 gttatcgaaa tatctgaggc ttctgaacac catccgtacc aaagccatgc aagatatccc
    14221 ggataagaga aaaagagctg cattgctcaa agggcttgct tccccaaaaa gcatgcgaat
    14281 gctttttcag aagggattcc gagaagcaag aagagctgcc ctcatctccc tgcataatat
    14341 cacccctcat taagacatgg ttctttgtcc tggattttag tataagttga gcgaattccc
    14401 cggggaaacc cgctggatgt aggatgtagt ataattagga tgtagtataa ttaggatgta
    14461 gtataataag ggtggggcat gaagaaaaat tcaatcaaga taacctcaaa gcgcggcaca
    14521 gagtttgatt cagaggtaat cctggggcag gaaaaatacc ttgtacagac cgaatggggt
    14581 ggtgctcaga aaccgcatat caccacccgt gtatatctca agggacaggt agtcttcacg
    14641 agaaaaaccg actgcagtga tatgatgaat tcctcggata tgccaagtga agtgcgggag
    14701 tgtatgctgg agcagcacca gtcagccata gccacactca aagcggaaag gtctgtaaag
    14761 acaaaaacaa cttcagacta tctgggtgag gtaaagaatt tcctcaggac caagaataaa
    14821 cgagccgctc tccgggtttt gaatgaagcc cttgagcatt atcctgacga ccccttcatc
    14881 ctctcttatt acggctgtct cgagtcagtt gcaaacaaga attacacaca tggaatagaa
    14941 acttgtcata tggcactgga gaatctgaag aagagggtgc cctttggaca ggagtttttc
    15001 taccccgtgt tttacctcaa tctcggcagg gcatatcttg ctgcgggtag aaaagatgag
    15061 gccatagaag ctttcaatag gggaatccaa attgacgggg agaacaatga cctcacatgg
    15121 gagataagaa aactcggtac aagaaggaag cctgcagtcc cattcatcaa gaggtccaac
    15181 cccataaata agtatattgg gatgctcctt cactccccaa gaaaataacc tcttttgctc
    15241 ttcgtaacat taagtataat agtaattatc cttattctca tggcgagttt tctgaaagat
    15301 atttaaattg aactatttct tgacaaataa ttattattag tttagaattg ttatatatgg
    15361 aaacttacaa acaaatagga tttaagctca cgccacaaag aatcgcgata ctcgattacc
    15421 ttaacgacaa caaggggcat ccatgcgcag atgaaattta cagagcggtt tcagagaagt
    15481 ttcccacaat gtcatttgct acggtgtata acaccctcaa cgcattgaaa aggcgtggca
    15541 tgctgcttga aataactatt gacccccgca agaaacggtt tgatcctgat tcagaacccc
    15601 accatcactt aatgtgtatt cggtgtaaga aggtggtgga tatccacaat gagtttgagc
    15661 tcacgatacc tctggggcag aggcatggct ttgaaataat gggcaaccac atagaatttc
    15721 atggtctttg cgaacagtgc agggaaacac gcagcgaaaa gaattcccag acctttcggg
    15781 gaggggacca ttagttggtt ttttgatacg aaggaatctt tgattcctca tgagagcaac
    15841 tgcacaggag aaagacaatg tcagaggtaa aaacagaatc aagagagtat gtgtgccagc
    15901 agtgcggata tatctataac cctgcaaagg gtgacagaaa aggaaagatt cccccaggcg
    15961 taccctttga agaactcccc gaggactgga catgccccct ctgcggtgca aaaaaagcaa
    16021 gattttcacc gatgttggat taaggaggaa gaactatgtg catggcatac gaaatgagat
    16081 gtaagtgcgg agacaaggcg gcgagttttc actttaagga caacatcctg tcagagcagg
    16141 tgataagaaa cctctactgt cctcgctgtt cttcagatat acagctcaat cctggctcca
    16201 tggttgtgga taatgggtgg gtcattgaat atgatatgga tgtggtgaac ttcatgggac
    16261 agaagatccg cacagggaca attaccccgg actctatatt tgatgagggc tattgtacat
    16321 ggaatggcct ctatcccggt gaccatatag agagcgttaa ggaacgtgag tgtatcgcgg
    16381 tccttgcaaa aacagatccc ctccgttacc tgaacgaaat gaagtcctgg gcaaatgaac
    16441 gagtgaaaag actgcagcaa gaaggctgga gaaaagcaaa accagaagag ctgtagaacg
    16501 cttaatagta ccatgcgctt ggtccagagg aatagttttc cccgagtttt cgagatgtgt
    16561 actacggagg acattatggt gagtaagaca atagaaaccg caatcaaaat ggaaactgac
    16621 gccatgaagt tttatgagga ggctgcaaga aaaacctctc atccttttgg gaagaagatg
    16681 ttcgagggat tcatcaagga tgaagttcgg catctccaga tgctggagga tattctgaag
    16741 ggactcaatc tcaccacagc taaagtcaac ccgtctgatg ggattaagac tgtattctcc
    16801 gaactgaaag accagatgat ggcccgggtg aaggcatccc aggacgaact ttccgcggtg
    16861 aatattgctc tcgacttcga gagtgcggga tacgaattct ataaaaaagc ggcccaagac
    16921 gcaacggaca aaaaggaaaa ggaactcctt gcaaggcttg cagaggaaga aggggagcat
    16981 tcatctatct tatggaatac ctattccttt cttagggata cgggagactg gttcatgtgg
    17041 gaagagcacg gcatactgga aggataaaag gggatagttt ccctttcacc taaaactcat
    17101 ctatagaaca agaaagaagg aggcagaaca ctatgtcaaa gacagagcag aaccttaagg
    17161 acgcattcgc aggagaatcc caggcaaaca ggaaatacct cgcctttgca aaaaaagcag
    17221 aagcagaggg ctttacgcag gtggcaaggc tcttcagggc agcggcagag gcagaaactg
    17281 ttcacgcgca taaccatctt aaagagcttg ggggcatcaa gagcacaaaa gagaaccttc
    17341 aagaggcaat taatggggaa tcgtacgaat tccagaagat gtatccccag atgataaagg
    17401 atgcagagtc agagggaaat aaaggcgcac tcaggagttt taattatgct aacgatgtag
    17461 agaaaataca tgcagccctt tacaagaagg ccctcgaaaa ccttggaaaa tcccctgatg
    17521 ccgacatgta tgtatgtcag gtctgcggtt atactgtgga aggggaggcc cctgatgaat
    17581 gtcctgtgtg taaggcaaag aaacaatcat tcaagaagat agattgataa agtactgagt
    17641 tcctcaggaa cacgcctcat gtaggtgttt ttcatatcag tacagaggga ggtcggggta
    17701 acggacctcc ttctctcttt tcagcctccg cacgaccggg attaatgaga ggctgttttc
    17761 cggggtattg gtgtatactg gaccatgggg gtaaggagaa aaacgacaac gcactccaca
    17821 atcaggaaga gacgtctgcg agagtctctt gcccaagaac gtttctttaa acattttatt
    17881 catggtatat aagcgtcttg aattgcatct gtctgtgcac gtgaggaggg gttgatgggg
    17941 ttggcaattt tttcagcctt tccccacgag ataagacccc tcgtcaagaa cctcagagct
    18001 gcacgaatat ccaaaacgct cccctttacc ctttttcttg ctccctatgc ctccggggag
    18061 gtgattgtgg tgctgactgg catgggcacc cctaatgcgg aggccgcact ggactacgtt
    18121 atcaaagagc attctcccac cattattctc tcctcagggt ttggcggagc actttacgct
    18181 gacgccgcaa taggagagct catatgggca tccagggttc ttcttgtgaa tgacagggtc
    18241 agggacgccc tggatctccc tgacagggac catctctacg atagactttc tcaaaaagtc
    18301 acgatacagg aaggaagcat cgttaccctt gaacgatgga tgaagaaacc cgaaatcaaa
    18361 cgtgccttat caaaatcact cctctttcct gtttgtgata tggaaacgtt cccccttgca
    18421 aaggccacaa tcacaagggg actgccgttt gtcgcaatcc gttccatcac agacagatct
    18481 cacgaagaga tcccgcctga actgctctgt gtagcggata agtcggggaa ttacaggctt
    18541 tcccgctcac tgaaagtgct tatcgggaat ccgtctctca tcccccgcgc gatcgacctc
    18601 ggaaggaatt ccgccatcgc gtcaaagaac ctctggcgcg cggtgagagc tcttatcgag
    18661 gtactgtaat ggtgatcacg acattttcgg cactgccgac agcggaaaat cctgcaggtg
    18721 aagctctatg gctttgcgaa tttcttcgga agaacgttta gcggtgagaa tatggtaccc
    18781 aagcactatg ggaaggagga cgatgaatac tcccacccac cccatgtgat actcttcgat
    18841 tgcaaaagtg accgagagat taaagatgag tatcccgaag tagagacccg ggcctatcag
    18901 atagcgccag ggaagcttat atcccgcata atcggtgcac gtatcgagga agcgatcaat
    18961 tttttgtaac acatagatca tcaggaaccc caccagaaac catccgatga aattggaaat
    19021 gggaacaccg aaatagaccc ctttctccgg atatccgtat acctgcccga ggaaccattt
    19081 atggccctgc agcgcaacag gatcgataat gatatcgaga tagacgaaga agagtgtacc
    19141 caggaaccgc gcataaagag agttcctgac cttctttgtc tcgaggagat atatccttct
    19201 ccccgaatac aggacaggag caataaccat aagagccatg gaatatcccg catacgcgag
    19261 gaatatgtaa ctcagagaat ccataaaggg cacacccaaa acccagagtt caagcccctt
    19321 ggtgtgctca atgtaataat agtacccgta ggggatacca ttgtggatag aggagtattc
    19381 agacacccat gcgataagat accctgccac aaaaaacagc agggccctct ttagcccaag
    19441 atgcattgaa cagccaaaaa ggtatatccc aagaaacacg aagacataag gtctcaatac
    19501 caccgtatgg aaaagaagag gaaaaaactc cattaggatt ttttcaggtt ccaaaggagc
    19561 agcctgatca ggtccccggg atgcaaaaag gcggtcttca ttactgacgc ctcatagccg
    19621 ctatgcacca tgcagttcct gcatcgcggg tcattccctg tgccgtactt ttcccagggc
    19681 gttttttcca tcagctcatc aaaggtatca tagtagcggt cagtgatgag atagcagggg
    19741 gacttccagc cgaggggatt acgcgtgggg tttccccagg gcacacagcg catttccctc
    19801 ctccctttaa ggaaatccac gtagaggggc gagctgatga agggaaaacg gttaaagaac
    19861 gatgcaatct ttccgaactt ctcccgtatc tcatccctgc tgaggaatat actcccttcc
    19921 acgctctcgt agctgaaggc gggagagatt aaaattccgt ccacatggat gctcttcagg
    19981 agctcaaaaa gccgctccag ctctcctgta tccgtggtct tgtatacaga agtgttcgta
    20041 cttacccgga accccttctc ttttgccctc ttgattgtct cgatagtctt cttaaacgtc
    20101 ccgggcctgc aagtgatctg gtcgtgggtc tcttccaaac cgtcaaggtg aaaattcagg
    20161 gtaagccttg ggtgagggta agcctcatca acaaaggaat cagtgagcag accattcgtg
    20221 cagagataga catatcgctt catcctgagg agttctgcca caaggggctt gaggtcagga
    20281 tataaaagag gttctcctcc tgtaacggtc actacgggcg cccctgattc aaccacagcg
    20341 cgtatgcatg tctcaaggga gaggtcctgg gactgctcct tgctttggag ccgtatcctg
    20401 tcgcatccag cacacgcgag attgcaacgg tgcgtgggct caagcatcag gacaagggga
    20461 aacctctttt tcccttttat taagtgcctg aaggcagcaa tggccaacga tgaatagaga
    20521 ctaatcggaa agcgcatgga ttatcgtttt gtaatcccct atatattatt ttttctcaac
    20581 catttttccc gtatcacact actcctttat ctcctgactt tctcaaaaaa ggcgtgcagc
    20641 ccttttcatc gcgccatcca tgaaactata gggtacgttc agtgcatgca ccgcctttga
    20701 ctagtcaatg aaatcctttg ccatgtcacc cggactttta tggctggctt ccgctttttt
    20761 gcagccttgc acagaactga ccaagggcca tgaggggaaa gcagttcctg taattatggt
    20821 atctcagata gaagtgcctg ggaaatccag tacccgtaaa ttcgctttca tcccatgtcc
    20881 cctcaggttt ttgacgttcc aggaggtata ctatcccttt tttcacctct tcacagccat
    20941 cctcacctgc agcaatcagt gccaagattg cccatgccgt ttgagaagga gtactcttcc
    21001 cgcgacatcc gcacgtccgg tcctcatagg attcgcatct ttccccccat ccgccatcag
    21061 aattctggtt gttcttcaag cacatgacag cccttcttac atagggactg gacatgtctt
    21121 cctttatggc acaaagcccc atcagcactg accaggtccc gtagaggtaa ttcactcccc
    21181 atctgcccca ccatgagcca tccttttcct gcgtccgttt taagaacgca atggcccttt
    21241 gcactgtgtc attcgtatac tcatatccca acaggccaag gagttccaga acccttcccg
    21301 tgagatccgg cgtgcttgga tctatcatcg cttccaggtc tccaaagggg agctggttca
    21361 gcagcttcat gtcattgtcc acatcaaagg ccccccatcc cccatctttt ccctgcattc
    21421 ccagtatcca ctggagacca ctctccatat tttcctcttt aacaaaatct cgatctgtgt
    21481 atctgttgag aagaagtaac acaacggcag tatcatccac atcagggtac cagctatttt
    21541 cgaactcgaa tgcccaccca ccggggttga gggagggcct cttcatactc cagtccccct
    21601 ttgtgaaaat ttgcttggag gcgagccacc tgcatgccct tataagagaa ggatggcttc
    21661 tttcctttcc tgagtagagc aatgccaaac ctgtgagggc agtatcccaa actggagaga
    21721 tgcatgcctg aaggcttagt tcatgctcat tctcgatgca aaaccttttc aaggcttcaa
    21781 gtcctttttg tatcggctcc gacgagactt cataccccat ggccatgagg gcgataatcg
    21841 aattgatcat ggggggctgt attcctcccc aatcaccgca tggttcctga tgttccagta
    21901 tccactgttc agccctcttg agcgctttct ccctcatggg ccttatcgga agagcttccg
    21961 cagtcttcag gagcttatcc tgtatgagga aaaatctctt ccatgaaaaa ggtggcaact
    22021 tctttggcgt gaggggaggc attttatggg gctccttata gagctccttg atccccgcaa
    22081 aatcattcaa tggcctgaca ggcttatact ccgcaatcac cgagagtggt acgaccgttg
    22141 tcctcgccca actcgaaaaa ttatagatgt tgataggaaa ccaggggggg aaaaggttca
    22201 tctgtacagg aattgaggga accgccttcc agtcgaattc gccaaacagg gcaaggaata
    22261 tctttgtaaa aacccttgag gcctccacgc ctccctcatt cagaatgaat gccctcgcct
    22321 ttttcaaagg ctcgtcatct ggagaaagcc ctgctattct gagggcaaaa tacgcttcaa
    22381 tggatgtact gaggtcccct ttcccttccc aatagagaga ccacgttccg tcagccctct
    22441 ggtgtttcag tatatggttt acaatctttc gatccctttc ttcatcccga agacccagaa
    22501 aatggagaag catcaggtat tctgaggtca ttgttacatt ggactcgagt tcataccacc
    22561 agtagccatc ctcgtgctgc tgagtcatgt aatacgaatg caccttccct atcgcttcgg
    22621 ttaccctgtc cagtaacata accggctctg caacggacag aacgttttct tctggatgtc
    22681 tcaacaagac cgcgggtttg gctctggagg attgcccgga atacccgttc ctcatgtttc
    22741 tgaacatagg atgttgtttt gtccttcctc tcactctgta ataggaaaag ctgttaccta
    22801 aaggccaaag acactcaaaa aacgttgccc tggcatatca aactcctgct ccctattggt
    22861 ctgaacaggg ggattacgaa tcacggagaa aatcagccga gacctttatt ccttttcatt
    22921 gaaattttac cacatctgtc cttttttgaa aaccttatga atggtgatca tcaaggctct
    22981 ggcgcacccc tggcccataa atatcctcaa aaaatacggg gatagaatct ccttaccctt
    23041 aattgccttt tgtgtgatta cgtctcccca ggagctccag caaggctggt aaaagaataa
    23101 gggtggagac taacataaag ctcaggcaga gtacgaggag catccccatg ctcgcgattc
    23161 ccctgtgagc ggagaaggaa aggctgctgg tgctgacaat ggtcgtgagc gtgctgaaca
    23221 gcactgcccg ggccgtactg gtcttcaaca tgttcccatc tggaggaggc tctgtgcgat
    23281 aacggagtac aaaaatgagg ccgctatgga cacccactcc caacaaaagc ggcacaatga
    23341 tgacattggc gaaattgagg ggaatgtcaa gcagggccga ggacacggcg gtcagaagca
    23401 ttgcgagcag taaaggaacg agaatcgtga tcgtgaagga gatgcttctc agttcaatga
    23461 gcaggtaaag ggtaatcaca acgagggccg aaagagctgc ttgccggaag gatgagacta
    23521 ccgccctccc tgattcatag acggtcacag gggtgtcagt tgcatcaggt gcgagagtac
    23581 gcaccgaatg aataaaccgc tcaagagcat ttctgtccaa aatgttctcg cggggaaaaa
    23641 cctggacacg ataccgcccg tccgcagaca catactgagt taccagatcc tggggcaagg
    23701 agcgcatatc aacagcgctt gcctggagta agttctccag tcgctcaagg agcgaaggga
    23761 ggctggacaa cagactttct tccagtgctg caaagacctt ttgcccattt tctgaattct
    23821 taagcatggt ttgaaaacgc ccgatatcct catacagacg ccgggcagag ctggcatcct
    23881 catatgacaa tgagccgagt gatttcttaa gcgccccctc aagtttgttc agtgaggtga
    23941 gcttctgctc gtagctgggg tgtttcacct gaaggttctt aagtgtgggg ggcatcatga
    24001 gacgtatatc agaaagaatc ctcagctttt cgggctgagc ttcgggtacg aaatcggaga
    24061 gggttattgc catcttcacc tctttcaggt ttcccagttt ctctgcaaga gcctttgcct
    24121 ctcccgcatc cttgacgagt atcgaaatcg tccatggtga agaattaggg tccttgaaaa
    24181 gatctttcac cgtggcgatc gcttctgact tcttgtcata cagattcaat ggattgtaat
    24241 caaaatatac cttgggaagg agaaacagag cccctaatcc cagtataatg gccacgctcc
    24301 gaatagcctt agggtattta taagggagtg tgaggatcat accccctaac gaggattttg
    24361 gcatatccct gcgttgaagg gggagtaagg tcaggagcgc gggaagtatc gtaaggtttg
    24421 caaagaaact gatgaacata cccatgcctg atatgagacc cagctcagcg accccggcat
    24481 aggcagtggg tacaaaggaa aaaaaaccta tggcgatggc gatgcaactc accagcaggc
    24541 ttctgcccac cccttttgct gtcatgccga tacactcccc atggtcaaag ccggatacca
    24601 ccaattccct gtaacgcagg cagaactgaa tgctgtaatc gataccaagg cctataaaga
    24661 gcaccgcaaa ggtgatggaa atcaggttga ggcttccgat aaatgccacc gcaaatcctg
    24721 tagtccagat gagcccgatt atcaacgttg tgaggctcgc gaacaccaat cgccctgacc
    24781 tcccgagacc tatatacaat gagcagccta caagaaacag agagaccagc gtggcaaccc
    24841 ctacgctatt tcttacctcg gtcaagttct catgggacag ggccacatca ccggtgatgc
    24901 gtgcagtcac cccgtgttct ccatgaaatc cgagttcctt gagcacacgc cgcactgttt
    24961 caagcggcgc ttcacctgaa gacagcttgg tgaaatcgag atagggctgc aggataataa
    25021 attgatggcg ctgctttggc agctccttgc cgaacatcat cccttgccag gagagatggt
    25081 acttgcggtt gttggtcacg ctctcgaagg cttcactcat cccgtcaaaa agaagcttga
    25141 tcctgaggtc cttgaggctg atctcctccc catggcccaa cgccatagtg agcatggaaa
    25201 agagaccctg cacgctcaaa tcttgggaaa gaagtgcgag gaagggctgg gcactggcca
    25261 gggtatcagc aaaatcgtcc aattccttta cactcaggta gagaagaccg tttttttcaa
    25321 aaaaacttcc ccctcccggt tcatagactg atttgaaaag acccgtttct tttcttagac
    25381 gttcggctaa ctgcgtgcgc gctgaccggg cgcgttcctc ggtatcggca tcgagcacga
    25441 tcacaatggt atctctcagc ttcggaaaga ccccggaaaa atccttttcg agctttcgaa
    25501 aatggagttt ttcggaaagc atgctggtgg tatcgacatt gatgttaaaa tgagtgaggg
    25561 aataagagaa tatcccaccg gtgatcagaa gcgtgatcac cattacaagg gatgcatggc
    25621 gctgcacaga ggtaacccac catacgagca gccgtgccgg ataatcctta gctcgtccca
    25681 tcatcctttt tgccttaaat caaaaaaacc tgttctcata atcatacgta ttaagttatt
    25741 atattatgcc aaaaaagaag aagtttctgg tgtgtagaaa attcgcggcg tctttatacc
    25801 actaatacag aatggagcag aaaagatgaa cgtggcaaaa caagagaaaa gtatgcgcat
    25861 ggcgcattgg ctaatcgtat caggtatctt cctgtataca gtactttttc ccctcccggg
    25921 aaaagcggcc tcagcgggtc cggcagaagt gatcaagaca ttcaatgcgg ccctccttga
    25981 gtccatgaag agggcggatg agcttggcta ttcaggccgt ttcaagctgc tggaccctgt
    26041 aatcaggaat tcttttgccc tttcttatat ggcggggcaa tcattgggcc ggtacgggaa
    26101 gacaataaag aaaacggagc aaagcgcgct ggtaaaaacc tataccgagt ggtctatcgc
    26161 tacctatgca agtcgttttg acgggtattc aggggaacgc tttgaggtgg tttctgagtc
    26221 gccatccgac cagggaacgg ttacggtgat cagtaagatt atccagcctg ataaagaaga
    26281 ggtggcgttt cactataagt tgcgcaaatt tggtggcaat tggcgtatcg tggatatcca
    26341 gatctcgggc gtgagccagc tcgcgttgac acgatcacag tttacgaacg tgataaagga
    26401 aaagggcttt gacggtctga ttgcattgct gaaaaccaag atcgagggcc tcgcacaagg
    26461 aaaaccctga tatggcgtcc tgccctgaac gtctttctcg ctggtactcc ctcatccccc
    26521 gttcttcacg taattcactt atccccttgc gcagcctgca tgccaaagca aagaggtttg
    26581 tgaagaatac ggcaaatccg tccgcttgta ctgtttccct ttgtctgtta gaatataacg
    26641 gtggttacaa aagggttgag gggttaagcc cggcgtaatc attgttgtgt tcaagagcac
    26701 gatggtcgcg tagccggctt gtcgcacatt gtgggtatca gggttcctct gaaaaaaaca
    26761 gaacgggaca cggggtatcg taatggaatc gattatgaag ggcagctcct tcgggcttct
    26821 tcatttcctt ggcggcagga gcctgcgtat catcaataat ctcggcgccg tggtaatttt
    26881 ctttgccctc tccttcctga agatcttcag gcccaagcaa atccgtgaga ttattcagca
    26941 ggtcttttat attggcgcca ggtcatccac tatcgtgatg ctcattggtc tcttcaccgg
    27001 catggtcctc ggtcttcagc tctattatac cctgataaaa ttcggctcta caggagtcct
    27061 tggttcggtc gtggccctgt ctctcatcag agagcttggc cctgtcctga ctgccatcat
    27121 gatcactgca cgggcaggct ctgcaatgac cgctgaactg ggaatcctcc gtatctccga
    27181 gcagatagat gccctctaca ccatgcgcat cgacccactt cgctatctca tcagtccgcg
    27241 tatcgccgcc tccattatca gcttcccttt gctcacagcc ttttttgatc tcatcggcat
    27301 cctcggagga tatctcaccg gcgtggtcct cctcgatgtg gataagggcg cctactttta
    27361 tcgcatccat tcctacgtgg agctggtgga catcaggggc gggttcatta aatccttggt
    27421 cttcgcagtg ctcgtgtcga cgatctgctg ctatcaggga tacttcactc atctccgtac
    27481 cgacagttac ggcgcaaaga gtgtgagcct ctcgaccacc tcgggagtgg tattctcctg
    27541 tgtcatgatc ctcatctcag actatgtcgt gacctcattt ctcatgtgag ggttgttatg
    27601 gatacgccgc tgatagagtt cagggaagtc acgaaacgct ttaacggaaa gaccgttctt
    27661 gacaggatga atctcatgat ttacgagaat cagatctcga ccatcattgg caaaagcggt
    27721 acggggaaga gcgtcctcct gaagcatatc atcgggctgc tcacccctga tgaaggcatc
    27781 atcctgttca gggggaaatc cattcatgcc atgaagaaaa gcgaacggga ctcgtacagg
    27841 agcaagatca gttatatgtt tcagaacaat gcccttttcg actccatgac ggtttttgat
    27901 aatattgctc ttcccctcag gcagacaacg gatttaggca agaaagacat tgagcagaaa
    27961 gtgacgacca ggatcgaaca gatggaatta tccgaagcgg ttaccaaatt cccttcagag
    28021 ctctccggcg gaatgcagaa acgggttgcc ctgtcccggg ccctcgtaac agacccgacc
    28081 atcgtgctct ttgatgagcc gaccacaggt ctggacccga tcaggaaaaa catgatcctg
    28141 agcatgatcg cccattacag gaagatgttc ggcttcaccg caatccttat cagtcacgac
    28201 atccctgatg tcttttttgt ctctgaccgg atcctcatcc tgtgggaagg aaagatcgtc
    28261 tttcagggga cctatgagga atccacacga cttaatcatc ccataatcga tgaatttctc
    28321 aagagcctcg agggggtcca ggacgaactc accggccttc tttcaaagga gatgttcaag
    28381 gcaagatatg ccattacttt gggcagggga cgtgtcgata cctctctcac ggccgttctt
    28441 ttcagtgtcc attttgacct cctcgtaaat gcgctcggcc cccaggctgc cattgaggcc
    28501 atcaagaagc tcggggagta tgtgaacgaa tatttcggcc cagtgggagg attctcggca
    28561 cgccacagga ggggtgaagt cctcaccatg ctcccccaca tagacattga agaagccatg
    28621 caattagtgg ataattttgc taaggggctt gagcaaaagg tcctccccga gatacaggga
    28681 ctcaccacag ccaaaatcgg cgccggggag tgcttcgaac tatttgtcac cgcgggaatc
    28741 atcgaaggaa cctcggctga tgacatcgat cagcttatag aaaaagcaga ggcatcgcag
    28801 aaggtaattg cacgatacca gtgtgttaag gaggtcagcg tataatgaga aaatactctg
    28861 cagaaacggt ggtcggcatt tttgtggtct tcggcctcct ctgcgtggcg tatatgaccg
    28921 tgaagctcgg gagggtctct ttatggggag atgattacaa taccttctat gcccgcttta
    28981 gttcagtatc aggattacgg gtcggcagcc ccatcgaaat ctatggcatc gaggtcggac
    29041 gggtaacccg actgaccata gatccggaga agcaggtggc attggtagaa ttgaagatcg
    29101 ggaaaggctt caaggtatac gacgacgcaa ccgcaaccat caagactgcc ggtctcatcg
    29161 gtgacaagct tgtcaagatc gatcccggag gcggcggtga tatcctgaag ccgggaggga
    29221 ccattaccga gaccacctcg cccacggata tcgaggacct catcagcaaa tacgcattcg
    29281 gagatgtaaa gaaagaagat aaaaaagaga ccaaatgagt ctccaggagg acgcaatgaa
    29341 gaaatccctc ataggaattg cactctttct ctgtgtgatc attccgctcc ctgtctttgc
    29401 aggtgtgccc ctcgacaccg taaagacaca cgtggataag gtgttggagg tgctgcgcga
    29461 tccatctctc aaggccgaat cgtccaagaa gcttaagaaa gacaaaatct gcgccatagc
    29521 cgaaaagatg tttgacttca ccgagctctc aaagaggacc ctagcccagc actggtccaa
    29581 gttcacccct gagcagcaga aggagttcac cagtctttac aagtcactcc tcgaaaatac
    29641 ttacgcagac aagattttgt tgtacaccga cgaaaagata ctctttacca aggaggttcc
    29701 actgaccgaa aaaacggccg aggtacagac catggtcgtc agaagtaccc aggagacccc
    29761 catctactac cgggtgattc tgaaggacgg tacctggatg gtgtatgatg ttattgtgga
    29821 agggatcagc atcattaata actatcggag tcagttcagg gagatactcg tgaacaaccc
    29881 ccctgagtac ctgctcgaaa ccctcaggaa aaaggtcggc aaggcctgat acatggataa
    29941 aacctcaaaa aaggagaaga cccaatgaaa cacatatgta ggttcgtact gactgtaatg
    30001 ttcgtgacct atcctgttat tctctgggca caggccttcg atgaaccaga cgcggcagtt
    30061 catacatccc cgggagttgt tcattccgat gccgggctga tgggctttcc ccagccttta
    30121 tctctgtccg atattgatgg ccaacgtgaa acgccagcca tcatcgtggc acaggccata
    30181 gcgggtgaag agatacctgc acgagagtta gcaacccatg caggaaaaga agcgccggaa
    30241 gagggcgaag aagaggtccc tctgatcgct gaccccatag ctccgttcaa caaagcgatg
    30301 taccatgtga atgataagtt atacttctgg cttctcaagc ccgtaaccca ggtatattct
    30361 cataccgtgc ccgaaccctt cagggtcgtg ttcaacaacg catttgacaa cctttgggct
    30421 ccgagcagga ttgtgaataa tctgctccag ctcaggttca aggcagcagg caatgagttc
    30481 atcagattca tcttcaatac cattgccggg atcggtggcc ttggcgatgt tgccgcgcct
    30541 gccctcggca ttaagaagca ggaggctgac tttggccaga ctcttggcca ttacggaata
    30601 ggccacggct tctacctcgt gctgcccgtc tttggacctt cctccgtccg cgacggcgtg
    30661 ggactggctg ctgaccggtt tatgtatccg ctttcctata taagcacgag tgacctgacc
    30721 tttggcgagg cagcaggaat ctatgcgggc gagaaggtaa acgatacgtc attccatatc
    30781 ggagagtatg agtctttcaa agactctgcc atcgatccct atgttggcat gcgtgacgcc
    30841 tttgtccagt atcgccagaa aaaggtggaa gactcaaaga aataggtttt tgttaccctt
    30901 aaagagactg gtgagccgtg gaggattcga acctccgacc cgctgattaa gagtcagctg
    30961 ctctaccaac tgagctaacg gcccattaaa gagagtgaaa agtgatcagc gaacagtaac
    31021 tagttaacca aaaaacaatc ttcccgtcaa cctaaactcg tcacgcaccg ctgatcactt
    31081 cagatattgt taagcaaaac ctttttggcg cgcctgagag gagtcgaacc tccgacaccc
    31141 agctccggag gctggtgctc tatccactga gctacaggcg cgatagaaac agctatcagt
    31201 aataagtgaa tagtgatcag tgaagatcaa attccttacc acagctaacc tgttcaccga
    31261 tgactaagaa ctgttctctc agattttgtg tggcaaaatc agttggggtg ggcgagggga
    31321 ctcgaacccc caacacctgg agccacagtc cagtgctcta accattgaac tacgcccacc
    31381 atgcaattgc acaaccggtg ttctgcaaga ttcaattata ccataatcaa aattccttct
    31441 tctatccagc cacatcctga gagcaggcaa aaaggccttg ccatccccca gttttttgaa
    31501 ggagggccgg ctgtggggta gatggagagc aagaggagcg ggattatgat aacgaggaag
    31561 aagaggatga cacgtggcga ggccaatttt atcagggagg atgcttatgg tttggtc
//
LOCUS       DATKOU010000185         5248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5253839, whole genome shotgun sequence.
ACCESSION   DATKOU010000185 DATKOU010000000
VERSION     DATKOU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5253839"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VMU21_08640"
     CDS             complement(<1..662)
                     /locus_tag="VMU21_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO77630.1"
                     /translation="MEKRYFGHIEIPLKRIHIELTNVCDFNCLFCPKSEMERPYGYME
                     TGLAKRIIDEIGEHGLTEKITFHVMGEPTLHPDFFDILSYTHEKGVKVGLTTNGGGLG
                     GEVGSRLLDHNLHQIDISLQTPDARSFTLRKARALTFDSYLEGILTFFHSYQSRWPAT
                     IFKFRFLNTRFSQKGIEKKVGPVRVISSTEELRNTFRYWVGRIYDILTVQGEQRELAM
                     KR"
     gene            complement(798..2561)
                     /locus_tag="VMU21_08645"
     CDS             complement(798..2561)
                     /locus_tag="VMU21_08645"
                     /inference="COORDINATES: protein motif:HMM:NF010032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HUO77631.1"
                     /translation="MPFDATKLADWQISEEAEKHMPTPEEWREKLGLQKDEMLPMGRL
                     SKIDFLKVINRLKKNPDGKYIEVTAITPTPLGEGKSTTSCGLMEGLGKRGVNVGGALR
                     QPSGGPTMNVKGTAAGGGNSLLIPMTEFSLGLTGDINDIMNAHNLAMVAMTARMQHER
                     NYNDEQLERLTKMRRLDIDPTRVEMGWIMDFCAQSLRHIIIGMGGRQDGYMMQSKFGI
                     AVGSECMAILSVANDLADLKQRLNHITVAFDKSGKPVTTGDLEVGNAMAAFMRNTINP
                     TLMCTAEYNPCFVHAGPFANIAVGQSSIIADRVGLKLFDYHVTESGFAADIGFEKFWN
                     VKCRFSGLKPHVSVLTTTVRALKMHGGGPKVVAGKALPDEYTKENLALVEKGCANMVH
                     MIKVIRKSGINPVVCVNRFYTDTDAEVAIVKKAAEAAGARCAESQHWLKGGDGALELA
                     DAVIDACNEKNDFKYLYPLEMKLRDRVALIAKEVYGADGVSWLPDAEAKAKMLEDDPK
                     YADYATMMVKTHLSLTHDPALKGVPKGWILPIRDVLIYSGAKFLCPCAGTISLMPGTS
                     SNPAFRRVDVDVNTGKVSGLF"
     gene            complement(2667..3122)
                     /locus_tag="VMU21_08650"
     CDS             complement(2667..3122)
                     /locus_tag="VMU21_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUO77632.1"
                     /translation="MLSPELLKEQTLLEDLDDSELKKIASIIKEIPFKKGETVFKEKD
                     ETRGIFLIHSGKVEISKVTPDGWKQTLAILTKGSFFGELSIIEHRHHEANAVALEDTL
                     ILLIRKEDFEKMESEDLLLATRIMKGLIFVLSKNLRRMNERFLKALISY"
     gene            complement(3241..5205)
                     /locus_tag="VMU21_08655"
     CDS             complement(3241..5205)
                     /locus_tag="VMU21_08655"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUO77633.1"
                     /translation="MKKNLLFYFVALLSTSLFVLLQLIDPKIIGEQLESKTYDLRLRL
                     RNLVKAQSPPRDIVIVAIDEKSIKEVGRWPWSRDVMAELVNSISDAKPKAIGIDILFT
                     ERESKVKDEELAEAIKKAGNVVLATAFFVPVEKEISVAPKDVPDFLWDSAFMEVKSQE
                     GIPWKQFAVKPESVMPSLPEFSSAASLGHAYALPDMDGVLRWKIMYLNYGDDCYPQFS
                     LQIARTALGITMKDMVLYAGSGIKLGDRFIPTDLSGRVLVNYWGREHSFHYKSAVDVM
                     KNTAAAKDLRDKIVLIGTSALATYDQKVTPFSGNFPGVEENATVVANILRDNFNRKSP
                     GTVEMIVILLTGIFLGLTLPRLRAILSASLAIGAVIFYVLLSCYLLIYQNLWINLLYP
                     VTNMLTIFTIQTVIRFFHEERKAKEIRQMFSSYVSPKIVKELIENPDKARLGGERRVV
                     TLLFSDIMGFTSLSERRQPEEVVSLLNEYFKEMADIIFRWDGTLDKFVGDEIMALWGA
                     PLEQPNHAELALRCALNMSEKLNELQEMWRQRGSDTFDVGIGLNTGEVIIGNIGATGK
                     KMDYTAIGDHVNLAARVEQLTRQYGTRILITENTYAHVEPLIKKGLLGHFELNELAKV
                     KVKGKENEVKILGLKGLTQREVPPQADVVP"
BASE COUNT         1215 a         1354 c         1290 g         1389 t
ORIGIN      
        1 atacgcttca tggccagttc cctttgctcc ccctggaccg tgagtatgtc gtagatacga
       61 ccgacccaat aacgaaacgt attccgcagc tcttctgtgg aagagataac cctgacagga
      121 cccacctttt tctcaattcc cttctgggaa aagcgtgtat ttaaaaagcg gaatttgaat
      181 atggtggcag gccatcgtga ctggtaggaa tgaaaaaaag tcaggatgcc ttccagatag
      241 gaatcgaaag tgagggccct cgcttttctg agggtgaagg agcgggcatc aggggtctgc
      301 agggagatat ctatctgatg gaggttgtga tcgaggaggc gtgagcccac ctcgccccca
      361 aggccgcccc cattggtggt gagcccgacc ttgactcctt tctcatgggt ataagaaagg
      421 atatcaaaaa aatcaggatg cagtgttggc tctcccatga catggaaagt gatcttctcg
      481 gtgagaccgt gctctccaat ctcatcaatg atacgttttg cgagccctgt ctccatgtac
      541 ccatagggtc tttccatttc tgacttgggg cagaaaaggc agttgaagtc gcacacattg
      601 gtaagttcaa tgtgaatcct cttcaggggg atctcaatat gaccgaaata tcttttttcc
      661 atccggggtc cttgtttgta gctcctgata ttatagcttt acgttccccg gcaaataaag
      721 cgagccacag gtctcataag aaaaagacct ccggaatgtc tttccggagg tctttcaaga
      781 gataaaacag gggcagttta gaacaatccg cttaccttcc cggtattcac atccacgtca
      841 actctcctga aggccggatt ggaactcgtg ccgggcataa ggcttatggt ccctgcacag
      901 gggcagagga actttgcacc ggagtagatc aatacgtccc tgatgggcag gatccagccc
      961 ttgggcactc ccttaagcgc agggtcatgg gtgaggctca ggtgcgtctt gaccatcatg
     1021 gtggcgtagt ctgcatactt gggatcgtct tccagcatct tggccttggc ttcagcgtcc
     1081 ggcagccagg agacaccgtc ggctccatat acttccttag caatgagcgc tacccggtcg
     1141 cgaagcttca tctccagagg gtagaggtac ttgaagtcgt tcttctcatt gcaggcgtcg
     1201 atgaccgcgt ctgccagttc taaggctccg tcaccaccct tgagccagtg ctgggattcg
     1261 gcgcagcggg cgcccgcagc ttcggcggct ttcttgacaa tagccacctc ggcatccgta
     1321 tcagtgtaaa aacggttcac acagacgacc ggattaatac cggatttgcg gataactttg
     1381 atcatgtgga ccatgttggc acagcccttt tctaccaggg caagattctc cttggtgtac
     1441 tcgtctggca gggccttgcc tgccactacc tttggaccgc cgccgtgcat cttgagggca
     1501 cggacagttg tggtgagcac tgatacgtga ggtttcaggc cgctgaaacg gcacttgacg
     1561 ttccagaatt tttcaaaccc aatgtccgca gcaaaacctg actcggtcac atggtagtcg
     1621 aacaatttca ggccgacacg gtcagctatg atagaagatt ggcctacggc gatgttggca
     1681 aaaggccccg catggacaaa gcatgggttg tattcggcgg tgcacatgag agttggattt
     1741 attgtgttgc gcatgaaggc tgccatggca ttgccgactt ccaggtcacc tgtggtgacg
     1801 ggtttaccgc tcttgtcaaa ggcaacagtg atgtggttca aacgctgttt gagatctgcg
     1861 agatcattgg ctactgagag gatggccatg cactcggacc ctacggcaat accgaacttc
     1921 gactgcatca tgtagccgtc ctgccgccct cccatgccga taatgatatg acgaaggctc
     1981 tgggcgcaga agtccatgat ccatcccatt tctacgcggg tagggtcgat gtcgagccgc
     2041 ctcatcttgg tgagccgctc caactgctcg tcgttgtagt tgcgctcgtg ctgcatcctg
     2101 gctgtcatcg cgaccatcgc gaggttgtgc gcgttcatga tgtcgttgat gtcgccggta
     2161 agccccaaag agaactcggt catgggaatc aacagggaat taccgccgcc ggctgccgtg
     2221 cccttcacat tcatggtggg accgcctgat ggctgacgaa gggcccctcc cacattcaca
     2281 ccacgtttgc ccaggccttc catcaaaccg cacgaagtag tgcttttgcc ttctcctaat
     2341 ggggtggggg ttatggcggt cacctcaata tacttgccgt ccgggttttt cttgagacga
     2401 ttaatgacct tgagaaagtc gatcttggac aagcggccca tggggagcat ctcgtctttt
     2461 tgcagcccca gcttctctct ccactcctcc ggagtaggca tgtgcttttc cgcttcttca
     2521 gaaatctgcc agtcagcgag ttttgttgca tcaaatggca tcttttgagt cctcctttta
     2581 tattgtagtt atgagtaatc agcaaacagt atgcagcaga aaagaacgct tttctgaata
     2641 ctggtcaccg tatgttaaat gcttgctcag tagcttatca gggccttcaa aaacctttcg
     2701 ttcatccgcc tgagattctt gctgagtaca aagatgagcc ccttcatgat ccgtgtggcg
     2761 agcaaaaggt cttcactctc catcttctcg aaatcctctt tccgtatgag aaggatgagg
     2821 gtatcttcca gggcaactgc attggcttcg tggtgcctgt gttcaatgat cgacaattca
     2881 ccaaaaaaac tccccttggt caggatcgcc agtgtttgtt tccatccatc aggcgtaacc
     2941 ttggaaatct cgaccttgcc agagtggatg agaaatatcc ccctcgtctc atctttctcc
     3001 ttgaagaccg tctctccctt cttgaatggt atttccttta ttatgctggc tatctttttc
     3061 agttcggaat cgtccaggtc ttccagaagg gtctgttcct ttaatagttc agggctgagc
     3121 ataggcacta atatagtgca aacatctttt tttttcaaga gaaaagtttg gaaacgaggt
     3181 catagtgcat gccatgagga aagacgcaag aatgagtaaa actggttgca gaataacaaa
     3241 tcatggtacg acgtcggctt gcggaggtac ttctcgttgc gttaatccct tgagccccaa
     3301 tatcttcacc tcattttcct ttcccttgac ctttaccttg gcgagttcat taagctcaaa
     3361 atggcctaac aggcctttct tgatcaaggg ctccacatgt gcatacgtat tttcggtaat
     3421 gaggattctt gtgccgtact gtctagtcaa ctgctccacc ctggctgcaa ggttcacatg
     3481 atcaccgatt gcggtgtaat ccatcttctt acccgttgcc ccgatattgc caatgatcac
     3541 ctcaccggta ttgagcccga tgcctacgtc aaacgtatcg cttccccgtt gtctccacat
     3601 ttcttgcagc tcgtttagtt tctccgacat attgagggcg catctcaggg cgagttccgc
     3661 gtgatttggc tgttcgaggg gagcgcccca caatgccata atctcgtccc ccacgaactt
     3721 atcaagagtg ccgtcccacc tgaagataat gtccgccatc tccttgaaat actcattaag
     3781 gagagagacc acctcttcag gctgcctcct ttccgagagg gaggtaaaac ccatgatatc
     3841 cgagaagagg agggttacta cccgtcgctc tcctcctaat ctcgctttgt ccggattctc
     3901 gatgagttct tttacaatct tgggcgagac atagctggaa aacatctggc gtatctcctt
     3961 tgcttttctc tcctcgtgaa agaagcgtat gacggtctga atggtgaaga tggtaagcat
     4021 gttcgtcacc ggatagagga gatttatcca gagattctgg tagatcagaa gataacaact
     4081 gaggaggaca taaaagatta ctgctccaat ggctagggaa gcgctcaaaa ttgctcgcag
     4141 tcttggcagg gttaatccca aaaatatacc ggtaagcagg atcacgatca tctcaactgt
     4201 gcccgggctc ttcctgttga aattgtcccg caggatattt gccacgacag tagcattttc
     4261 ctcaaccccc gggaagttgc ccgaaaaggg agtgaccttc tggtcatagg tggcgagcgc
     4321 ggatgtccct atgagcacga tcttgtccct caagtctttt gccgcagccg tattcttcat
     4381 gacgtccact gctgatttat aatgaaagct gtgctctctg ccccagtagt ttacgagcac
     4441 ccttccgctc agatcagtgg gaataaaacg atctcccagt ttaattcccg aaccagcgta
     4501 gagaaccatg tctttcatag tgattccgag ggcagtcctg gctatctgca gagaaaactg
     4561 gggataacag tcatcaccat aattgagata catgatcttc cacctcagca caccgtccat
     4621 gtcaggtaac gcatacgcgt gaccgaggga tgcagctgag gaaaattcag ggagggaggg
     4681 catcacgctt tcgggcttta ccgcaaattg tttccaggga attccctcct ggctttttac
     4741 ctccataaac gcggaatccc agagaaaatc cgggacatcc ttgggcgcta ccgaaatttc
     4801 tttctctacg ggtacaaaaa aggccgtggc aagaaccaca ttccctgcct tcttaatggc
     4861 ctctgcaagt tcttcatcct tcactttgct ttccctctcg gtgaaaagga tatcgatccc
     4921 tattgccttt ggcttggcat cagagatgct attcacaagc tctgccatca catccctgct
     4981 ccaaggccat cttccgacct cctttatgct tttttcatct atggcaacaa tcacgatatc
     5041 tctcggcgga gattgcgctt tcacaaggtt tctcagccgg agcctcagat cataggtctt
     5101 gctctcgagt tgttcgccaa tgatcttggg atcaataagc tggaggagta cgaataggga
     5161 ggtggaaagc agagcgacga agtaaaatag gaggtttttc ttcattggta atgattatac
     5221 agggttttgg aaatggaaag acaggggg
//
LOCUS       DATKOU010000186         3976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2569723, whole genome shotgun sequence.
ACCESSION   DATKOU010000186 DATKOU010000000
VERSION     DATKOU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569723"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..986)
                     /locus_tag="VMU21_08660"
     CDS             complement(114..986)
                     /locus_tag="VMU21_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUO77634.1"
                     /translation="MKTTIACGLLGAGKTTFIQEVAKHTQEKTVVLVNDFGKVGIDGE
                     IFSADGIESIELPSGCVCCTLKFDLITTLQKITETLKPEHLIIEPSGVASPSAVLEAL
                     DLVKINPVTVVGIVDATEFLELHESQMFGNFFQDQIVNSDIILVNKTDLADEGTIARI
                     VQILENMNPRGVIFPTVKASLNGPLPAASRTESAVRKFETHIHLDTFTANITRTEPLA
                     FYKNLFSEIMEGTFGNVVRAKALVQTDEGPFRLDVSSGRLDCIPFERTVTHSRLVIIG
                     EDLKKEILLLLSKG"
     gene            complement(995..1468)
                     /locus_tag="VMU21_08665"
     CDS             complement(995..1468)
                     /locus_tag="VMU21_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO77635.1"
                     /translation="MGFDELVADLRKGTGLSRAKAAAGLGKLKDKRASGILIEALKDP
                     NAGVRNNAAFALGELDATEAVPHLIALMEDPEEWVRKSAAKALGMLNAAQAVIALDLA
                     TNDPSSVVRKNAIRSLGKIGGDAALLALKRIAADRDRAAAQLAQEALETLKTGSY"
     gene            1605..2120
                     /locus_tag="VMU21_08670"
     CDS             1605..2120
                     /locus_tag="VMU21_08670"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUO77636.1"
                     /translation="MKDDKERDLKEIVARRIEFFKKKPEAAIYKPTVSSKHIGALYTE
                     TKVREHLVKSDYGEAAGGTNLAPNPIELLLSAIAACIEAAFYEFAVHEGFTINALSVD
                     VEGTLDLRGLFMIDDVAAGFREVKYRFTIETPEDEEKIRQFAERVIAHCPVVDSLIHP
                     TSISGEIHVRK"
     gene            2217..2618
                     /locus_tag="VMU21_08675"
     CDS             2217..2618
                     /locus_tag="VMU21_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77637.1"
                     /translation="MSRLIILYSPRGINIAFSLSMAQILLCTHNPILVKDLYGILRDE
                     GFTVEIADHPALAVRMVLQGNYGAVIIDSSAFGLSAEEAVQIIRSISPDIPIIVAGNE
                     TYTGGALSLKTPVDLEEFKKTIHNFISLVKS"
     gene            2638..3687
                     /locus_tag="VMU21_08680"
     CDS             2638..3687
                     /locus_tag="VMU21_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HUO77638.1"
                     /translation="MSMTPREMILKAFEMGKPERVPVTLFGGGMWSIKDYGSTFEALA
                     LNPDKMTDMCVVESERIKSDVVYVGSGYNNFHASSLGKDLGVGIKYREIGAPDLTAHF
                     VHSEEDIAKLKIEDVHKDPVIKTVLEATRRVKEKIGKKYLVTMTCWGPFTLAARFVGE
                     EAFMKNTFKKPAFVEKMVDFCADLLIHLYEPLVNDGSLECLSIADPTASGDLINPKQM
                     ERFAVPPLKKMSDWAKSKGVHTILHICGNTSDRLEMFLKTGCTVIFFDQKVDIAKAKE
                     VLFGKMCFGGNVAPVHVLLNKTAAEVEQACKETIEIAGKDGGYVLVPGCDIPPTIPYE
                     NLKVFHDVAFNWKYK"
     gene            3767..>3976
                     /locus_tag="VMU21_08685"
     CDS             3767..>3976
                     /locus_tag="VMU21_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77639.1"
                     /translation="MGIFDAIKQFLAMGPESHEKEENKMAEKKMTVEEVNEYMKQKCG
                     FVPRMFQIINTVTPDPGRTFADFYAT"
BASE COUNT         1005 a          926 c         1081 g          964 t
ORIGIN      
        1 cacattttct tggaaagaaa cacaatcgca atacaagacg cggtgagtag tatcaggtgt
       61 attcgaaatt taggatactg cgtcttcccg gacggatcct tcagaacctg cgcttaccct
      121 ttcgacagaa gcaggaggat ctccttcttt aaatcctcgc cgatgatcac gagccgactg
      181 tgggtgaccg ttctttcaaa gggaatacaa tcgagtcttc ctgaagaaac gtccagcctg
      241 aagggccctt catcggtctg cacgagggcc tttgccctta ccacatttcc gaaggttccc
      301 tccatgatct cactgaaaag attcttgtaa aatgcgagtg gttcggttct tgttatattc
      361 gcagtgaagg tatcgagatg aatatgggtc tcaaacttcc tgacggctga ttcggtgcgg
      421 gaagcagcgg ggagaggacc attgagggac gctttgaccg tgggaaagat cactccccgg
      481 gggttcatgt tctcgaggat ttgcacgatc ctggcaatgg ttccttcatc cgccaggtct
      541 gtcttgttca caaggatgat gtcagaattc acaatctggt cctgaaaaaa attcccgaac
      601 atctgtgatt catggagttc aaggaattct gtggcatcga caatgcccac tacagtaacc
      661 ggattgatct tcacgagatc aagagcttcg agcacagctg agggggaggc aaccccgctg
      721 ggttcaatga taagatgctc tggcttgagg gtctctgtaa tcttctgcag ggtggtaata
      781 aggtcaaact tgagggtgca acagacacat ccgctgggaa gctctatgga ctcgatacca
      841 tcggcagaga atatctctcc atcgatgccc accttgccga aatcattgac gaggacgacg
      901 gtcttttctt gcgtgtgttt cgcgacctct tgaataaacg ttgtcttccc tgcgcctaat
      961 aatccgcatg caatcgttgt tttcatgaag tgcgtcaata agagcctgtc ttaagcgttt
     1021 ccagtgcctc ctgcgctaac tgggccgctg ccctgtcccg gtctgcggct atccttttaa
     1081 gagcaagcag tgcggcgtct ccccctatct ttccgaggct ccttattgcg tttttcctca
     1141 ccacagagga gggatcattc gtggcgagat cgagggctat gactgcctgg gctgcgttaa
     1201 gcattcccag tgccttggca gcgctcttcc gtacccactc ttcagggtcc tccatgagcg
     1261 caattaagtg gggaacagcc tctgtggcgt ccagttcacc gagtgcaaat gcagcgttat
     1321 tcctcacccc cgcattgggg tctttaaggg cttctatcag gattcccgat gcccttttgt
     1381 ccttaagctt tcccaaaccc gcagctgcct tggccctgct caggcctgtg ccttttctga
     1441 ggtctgccac cagttcatca aatcccatta acccctcccg gttgttccac ggtgctttaa
     1501 aacagagtga actgctatca ttgattactg agaccatata gtctaaaaaa atggtattct
     1561 ttttgcaatc attgagcaag aattataaga aagggggcag agggatgaag gacgataaag
     1621 agagggacct gaaagagatc gtggcaagga ggatcgagtt tttcaagaaa aagccggagg
     1681 ccgcaatcta caaaccgaca gtatcctcca aacacatagg ggctctttat actgaaacca
     1741 aggtaaggga gcatcttgtg aagtctgatt acggagaggc tgcgggggga accaacctgg
     1801 cacccaaccc cattgagcta ctcctctctg caatcgctgc gtgcattgag gctgcatttt
     1861 atgagtttgc agttcatgag gggtttacca tcaatgcgct ctcggtggat gtggagggga
     1921 cactggacct aagggggctg ttcatgatcg atgatgtggc tgccggcttc agggaggtga
     1981 agtacagatt caccattgag acaccggaag acgaagaaaa gatacgtcag tttgcagagc
     2041 gggtgattgc gcactgtcct gtggtggaca gcctcataca cccaacgagt atttctggtg
     2101 aaattcatgt gcgaaagtga gaagtggaag aggcctgagt tacagtagtg tggagaaata
     2161 ccccagcgga ttggggcatt tttccgccaa tgcaccatag gggattttgc aggggtatgt
     2221 cccgtctcat aattttgtat tctccccgtg gcattaatat tgctttttct ctctctatgg
     2281 cgcaaatcct cttatgcaca cataacccca ttctggtaaa ggacctctac gggattctaa
     2341 gggatgaagg cttcactgtc gaaatcgcag accatccggc tcttgcggtg cgcatggtcc
     2401 tccaggggaa ttatggtgca gtgattatcg attcgagtgc cttcggtctt tccgcagaag
     2461 aggcggtcca gatcatcaga agcatttcgc ctgatatacc cattattgtg gcaggaaatg
     2521 agacttatac cggcggggcc ctgagcctga aaacaccggt tgaccttgaa gagttcaaga
     2581 agacaattca taatttcatc agtctggtaa aatcctaggg aacaaaggag gagaacgatg
     2641 tcaatgacac cgagagaaat gattttaaag gcctttgaaa tgggcaaacc ggagagagtg
     2701 ccggtgaccc ttttcggagg ggggatgtgg tcaatcaagg attacggatc gaccttcgaa
     2761 gcgcttgcgt tgaatcccga caagatgacc gatatgtgtg tcgtcgaatc cgagcggatc
     2821 aaaagcgatg tcgtctatgt aggctcaggc tacaacaatt ttcatgcatc gtctctgggg
     2881 aaagacctcg gtgtggggat caaataccgt gagataggcg cccctgactt aaccgcccat
     2941 tttgtccatt ccgaagagga catcgcaaag ctcaagattg aggatgtcca taaggaccct
     3001 gtgatcaaga cggtcctgga agccacccgg agggtaaagg aaaagatagg gaaaaagtat
     3061 ctggtcacca tgacctgctg gggccctttc accctggcag cgcggttcgt gggtgaagag
     3121 gcatttatga agaacacctt caagaaaccc gcctttgtgg aaaagatggt ggatttctgc
     3181 gcagaccttc tcatccatct ctatgagcca ctcgtgaatg acgggtcgct ggagtgtctc
     3241 agtatcgcgg accccaccgc atcgggtgac ctgataaacc cgaagcagat ggagcggttc
     3301 gcggtccctc ccctcaagaa gatgagcgac tgggcaaaat caaagggggt ccacactatt
     3361 ttgcatatct gcggcaatac gagcgaccgt ctcgagatgt tcctgaagac gggatgcacg
     3421 gtaatattct tcgaccagaa ggtggacatc gcaaaggcga aggaggttct tttcgggaag
     3481 atgtgcttcg gagggaatgt ggcccctgtg catgtgcttc tcaataaaac ggctgcagag
     3541 gtggagcagg cgtgtaaaga gacgatcgag attgcgggca aagatggggg gtatgtgttg
     3601 gttcccggct gtgacatacc gccgaccatt ccgtacgaga accttaaagt tttccatgac
     3661 gtggcgttta actggaagta taaatgatat ccacattgac tgcgagggga ggtgaagaac
     3721 gaagggaacg aggagaagga tcaccctgtg ataggggagg tggaatgtgg gaatttttga
     3781 cgcgatcaaa caatttctgg ccatggggcc ggagagccat gaaaaggagg aaaataaaat
     3841 ggcagagaag aaaatgacag ttgaagaagt gaacgagtac atgaaacaga aatgcggctt
     3901 tgtgccgagg atgttccaga ttatcaatac ggtaacccct gaccctggca ggacctttgc
     3961 ggatttttat gcgacc
//
LOCUS       DATKOU010000187        13555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2176949, whole genome shotgun sequence.
ACCESSION   DATKOU010000187 DATKOU010000000
VERSION     DATKOU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13555
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176949"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..194)
                     /locus_tag="VMU21_08690"
     CDS             complement(51..194)
                     /locus_tag="VMU21_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77640.1"
                     /translation="MERHFNESISVLAPLKAKIKATDNLIDQIVYDLYGLTPEEIEIV
                     EGR"
     gene            complement(474..800)
                     /locus_tag="VMU21_08695"
     CDS             complement(474..800)
                     /locus_tag="VMU21_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO77641.1"
                     /translation="MKYEVRITGVAQDDLYSIYRFIANNDSPGKAEHLLDNIERAMAS
                     LERMPARGNHPPEMQRWGILEFREIFFKPYRIIYEIKDKAVFIHAVLDGRRSCEDLLQ
                     QRLLGA"
     gene            complement(797..1081)
                     /locus_tag="VMU21_08700"
     CDS             complement(797..1081)
                     /locus_tag="VMU21_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HUO77642.1"
                     /translation="MKLSTAVKPISHIKSHASEIISEISETGNPVVVTVNGEARVIIQ
                     DIKEYEKDRESMAILKILAMGRKDMREGRHKPIHQTFAELKKRISAAGKP"
     gene            1185..1478
                     /locus_tag="VMU21_08705"
     CDS             1185..1478
                     /locus_tag="VMU21_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77643.1"
                     /translation="MNPYSRLIPRLNGKEIEERFEYYLDLVKKGVAGFIVFGGELETV
                     RHGIRKLHDASHQPLIIASDLKQGPAQAWRKPRKFKRPGRGVKKAEGLCPDFF"
     gene            complement(1458..1661)
                     /locus_tag="VMU21_08710"
     CDS             complement(1458..1661)
                     /locus_tag="VMU21_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77644.1"
                     /translation="MKREYDFSKAVQGKFYRPVEQIEAPVYLDKEVKAFFSKKAAKKR
                     VRLEKIVNAVLRKEMEMLKEIGA"
     gene            complement(1666..1956)
                     /locus_tag="VMU21_08715"
     CDS             complement(1666..1956)
                     /locus_tag="VMU21_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HUO77645.1"
                     /translation="MNFKWDLKKAKANEQKHKITFLEACYVFADKCLLTLLDEEHSSK
                     EERWITIGQSDSGKILVVVHTYRKAKGTETIRIISARKATKNEEKQYCERRG"
     gene            2124..3527
                     /locus_tag="VMU21_08720"
     CDS             2124..3527
                     /locus_tag="VMU21_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HUO77646.1"
                     /translation="MNPYSRLIPRLNGKEIEERFEYYLGLLKKGVAGFIVFGGELETV
                     REGIKKLQGASHQPLIIASDLEQGLGQQIEGGTLFPPAMAAASAIKTARRDVASQLLK
                     RLYTAFAAEARYTGINTILAPVLDINTNPLNPIIATRAFGEDPESVSFFGCEMIRVLQ
                     ENRIMSCGKHFPGHGDTEIDSHITLPVIRKELAALGYHELIPFKRAIHQGVGMVMLGH
                     LSVPSLDPSGKPASISDKVVSYLRNNMTFKGLVITDAMNMGALGGYHENEASLIALNA
                     GVDLLLHPTDPDNTALFLRQKNYQPHPLTLNLTKSHSTVHFDSREHGELSDEISRMAI
                     TIEREYQGKIKKPFVLLVHDEHIKKGDVFLNEMKHAYPAINYRAIFPEEDIPLSAFPE
                     DCDLIVCVFSKIKAWKGTHAVWLRRTIQHLAGRARLFISFGNPYVIKGLENVTKIYAF
                     WDSDSAQKAVAERIIAT"
     gene            complement(3630..3791)
                     /locus_tag="VMU21_08725"
     CDS             complement(3630..3791)
                     /locus_tag="VMU21_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77647.1"
                     /translation="MPEEPKLAEELKKMEYEPLLPVEKKLISYSLIIGAALLGMLVWV
                     SYTFFPGGH"
     gene            complement(3808..5547)
                     /locus_tag="VMU21_08730"
     CDS             complement(3808..5547)
                     /locus_tag="VMU21_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HUO77648.1"
                     /translation="MEEKKQGISEDWLSLWLGLFIFVISLFAFAGQDLLGWGISTKTW
                     TDVSKATSATTKSYVMVKGEITKIDGQKVTLKKADGKEETITVKDTTALKVGDTYEAG
                     ISGFTSLLLTYLFMLVVMLVGAAALRVNIGKFIIGFTIVFWVSYACWLIGNYAYIAAT
                     DAKKAGVPWSLKLTGESGFIVALIAGLAVGNFFPKLSAYLKDVIRPELYIKTAIVLMG
                     ALLGVKAAESFGLATAVMFRGLCAIVEAYLIYWALVYFVARKYFKFSREWAAPLASGI
                     SICGVSAAIATGGAIRARPIVPVMVSSLVVIFAVIELIVLPFMAQTFLWKEPLVAGAW
                     MGLAVKTDGAAVASGAVTDALVRAKALTMEGVNYKEGWLTMTATTVKLFIDIFIGVWA
                     FILAYIWCAKIECKPGEKVGAGEIWNRFPKFVIGYVITFILLLLVCLPATRTVLPVEK
                     EIGPLKEEVAKSEKQIPTTTDPVALAALTEQINAAKDKIKGLEGQIKEPKATLANAKV
                     ATDGSNSFRVMFFLLTFFTIGVVSNFKKLWEEGIGKLAAVYILCLFGFIIWIGLVISW
                     IFFHGVKPPIVTG"
     gene            5951..6445
                     /locus_tag="VMU21_08735"
     CDS             5951..6445
                     /locus_tag="VMU21_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HUO77649.1"
                     /translation="MLLSKPVFPKDLPIHKFPLGTLSLYNIHTDERLEVTYRKASGEY
                     DQEALRALNWVMRCHYTGQVADMDVNVIEFLNMVDKQLGGNNDIHVISGFRSHEYNQL
                     LVERGRHVARNSLHVQGKAIDISIPKVDLVLLKDTALNLRYGGVGYYPRDFVHLDSGR
                     FRTW"
     gene            6658..8094
                     /locus_tag="VMU21_08740"
     CDS             6658..8094
                     /locus_tag="VMU21_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUO77650.1"
                     /translation="MQATPLMSHVDENRKNVVSFLMAYKDLREFIKILEKRALLRRIS
                     VEVDPILEIGEITDRMCKSPGGGKALFFEKVKGSSYPVVTNMFGSFERMSLALEVEKL
                     DDVARRIEELLNQAAPKTLMEKLAMLPKLFEFSKYLPKEVNNGPCHEIIEKENPDLSR
                     FPVLKCWPGDGQPTDEGRFITLPMVFTRDPDSGRPNCGMYRIHIYDKTTTGMHWHIHK
                     DGARHYDRFRSLNKRMPAAIAVGSDPSVIYAASAPLPESIDEMLFAGFLRKEPVEMVK
                     CITSDIEVPANSELVIEGYLEPGEMRIEGPFGDHTGFYSAADYYPVFHATCITHRKDM
                     IYPATIVGKPPMEVVFHNAALISIKKSYPGHAKKIIHGLWGKGQMMFAKLLVIVDDDV
                     DVQNISYTAWRVLNNVDWKRDVVIAEGPLDDLDHSASWPRYGSKMGIDATRKTREEGM
                     MREWPQELSMSEEIKKRVDARWREYGFE"
     gene            complement(8179..8730)
                     /gene="pth"
                     /locus_tag="VMU21_08745"
     CDS             complement(8179..8730)
                     /gene="pth"
                     /locus_tag="VMU21_08745"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUO77651.1"
                     /translation="MWLIVCLGNPGRRYSKTRHNVGFMVADDSASRYGIELKTRESYM
                     IGKGSIEGNKVILMEPLTFMNRSGFAVKEVMKKYPLGPENLIVVHDDLDMETGKLKIR
                     KKGSSGGHRGVDSIIESIGTREFVRVKIGVGREEGVPAEEYVLRKFGKDQIPLIKDAI
                     VRASDAIAMVLRDGVDKAMNEFN"
     gene            complement(8731..9393)
                     /locus_tag="VMU21_08750"
     CDS             complement(8731..9393)
                     /locus_tag="VMU21_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HUO77652.1"
                     /translation="MERITLRAETRDKTGKGVARSLRRQGAVPAVIYRGGSSLPIKID
                     KKELISFIHATAGEQVMVNLQFAGGENKVALMKDYQVDPLKGELLHTDFFEVSLTEEI
                     RVTVYITTTGEPIGVKRDGGILQHGVREIEIQCLPDKIPGHVEVDVSSLEIGKAIHVG
                     DLPIGEGIEILTDPGDLVATVVAPVVEEVAAPAAAAAAPEVAEPEVIKKGKKEEEAGE
                     KA"
     gene            complement(9405..10346)
                     /locus_tag="VMU21_08755"
     CDS             complement(9405..10346)
                     /locus_tag="VMU21_08755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HUO77653.1"
                     /translation="MPEGIKVISGSSHRGLVQEVVDCLGISMCDTTITTFSDGEIMIQ
                     INENVRGTDAFVIQSTCPPVNDNIMKLLLIIDALKRASAGRITAVVPYYGYARQDRKV
                     QPRVPISSKLVADLITAAGANRVLAVDLHAGQIQGFFNIPVDHLYASPVLLDYIRGRN
                     LRDLVIVSPDAGGVERARAFAKRLQCSLAIVDKRRELANVSQVMNVIGDVKGRDTLLL
                     DDMIDTAGTTTQAASALKEHGAHRVFAASTHAVLSGPALDRINSSVIEEVIVVNTIPL
                     DSKKEQCSKLTVLSIAPLLAEAIKRIHEESSISSLFG"
     gene            complement(10431..10505)
                     /locus_tag="VMU21_08760"
     tRNA            complement(10431..10505)
                     /locus_tag="VMU21_08760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10471..10473),aa:Gln,seq:ttg)
     gene            complement(10548..11414)
                     /gene="ispE"
                     /locus_tag="VMU21_08765"
     CDS             complement(10548..11414)
                     /gene="ispE"
                     /locus_tag="VMU21_08765"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HUO77654.1"
                     /translation="MTAKMLTVNAPAKINWFLNVLGRRKDGYHDIVSLMQCITLSDSL
                     VMENSPRVEVLTGAEIPREENLVYKAAKLMREKTGVVSGVKITLHKEIPLAAGLGGGS
                     SDAAATLIGLKRLWNLDCTPRDLAGLGEALGSDVPFFFHGPVSVVQGRGEVVSPVELT
                     KSYHLLLVKPEISISAAWAYAEYDKVSQSREVLTKKHNNIKLFCQALGREDFAFLSSL
                     QGNDLEPLVVRRYPVIAEIKRSLMLRGALFSSMSGSGSSVFGVFDSERHAENAREYMS
                     PHWCRVVRTLIH"
     gene            complement(11596..13296)
                     /locus_tag="VMU21_08770"
     CDS             complement(11596..13296)
                     /locus_tag="VMU21_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77655.1"
                     /translation="MSVFVRMLILALAALTLSCASPGVKDQQRTPEPLPEPPAGAYYE
                     YMLGYEAELSGDWDEALKHYNEALRLDPSSYYLKTQMSYIYLRTGRVQDAIRTMEDVV
                     RADGTYVPALMLLAQMYNSQKRLDEAISLYEKVIKIDPSVSDAYLFLGVLYASQQSYD
                     KAVNILESLNKQDSGVVMASYYLGVIYMETKRYDKAEEYFKKVIDLKPNFEPAYFNLG
                     VIHEMNEDLSQAETYYGKAAELNPHDMRAKERLSQVFIKQKSVDKAIAQLRELSDQDP
                     ANIDVHLRLGLLYLEDKLYDRAIEELRLVLEAKPEDTAARFYLAIALEEIEKYPEAVD
                     ELKKIISLEPKNLDAFAHLGLLYAKLKQFDNAIKTYEEVLSFEKDKPEIYLYLGMTCI
                     EAKDFKKAEEVFKEGLALFGDNEDLNFNLAVLYEKTKRFDEMESYLLKVIGKNPKNAD
                     ALNFLGYSYADRGIHLEEALSLIQKALEIKPGNGYILDSLGWVYFKLGKNEDALRYLL
                     EATEGVKDDPVLYEHLGDVYSSMGNKDAAFKAWRKSMEMGEKEEGLKERVEEKVKGLK
                     "
BASE COUNT         3402 a         3543 c         2952 g         3658 t
ORIGIN      
        1 catagtctgt tgttaggata gcaaaaaatg tccttttcag caacattatg ttacctcccc
       61 tccacaatct cgatctcctc cggtgtgagg ccgtagagat cgtagacaat ctggtctatc
      121 agattatccg tggcctttat ctttgctttt aatggtgcca gcacggatat gctctcattg
      181 aaatgccttt ccaatgcctc ctgaattttc cggcttgttg tatcaacaga tatcttactt
      241 caattatggc atcaaaaata tctgaaactt tttctttttg tgctttccat aaagaagttg
      301 tgggcagatg atgttcgagg tagttgtttg agaaaaggtt ttggttgttc cataggaata
      361 actttgtctg ttcagccata cgctaacgta tcaggatttc tactattttt caagtaatgc
      421 ggaaagcagc aagaatctgc caactgtgta acctgcagac tgaagcgggc gaatcatgct
      481 cccagcagcc gttgctgcaa gaggtcttcg cagctgcgcc tgccgtcaag gacggcgtga
      541 atgaaaaccg ccttgtcttt aatttcgtat ataatgcgat atggcttaaa aaatatctct
      601 ctgaactcca agatccccca gcgctgcatc tcgggcgggt gattgccgcg cgcaggcatt
      661 ctctccagag atgccatggc cctttcaatg ttgtcaagca gatgttcagc cttccccggc
      721 gagtcattgt tcgcgataaa cctgtagatc gaataaaggt cgtcctgtgc aaccccggta
      781 atacggactt cgtatttcac ggctttccgg cagcgcttat tctctttttc aattctgcga
      841 atgtctgatg aatcggtttg tgcctgcctt cacgcatgtc ctttcttccc attgccagta
      901 tcttcagtat tgccatgctc tcgcggtctt tttcatattc ctttatgtcc tggattatta
      961 cccgagcctc tccgtttacg gttacgacaa ccggattgcc ggtctcggat atttcgctga
     1021 ttatctccga tgcgtgagat ttgatatgac tgatgggctt gacggctgtg ctcagtttca
     1081 tcccttcctc cttatggtca gaattaagtc tttattgtag actatcagat attgaggaaa
     1141 aagcaaagcc ctggcagcag gcaggtcttt tgctataata ctccatgaat ccttattccc
     1201 gcttaatccc gcgcctgaac ggcaaagaga tagaagagcg atttgagtat tatctcgatc
     1261 tcgtgaaaaa gggcgttgcg ggtttcattg tattcggcgg tgaacttgag acggtacgac
     1321 atgggattag gaaactccat gatgcttctc atcagccgct cattatcgcc tctgacctta
     1381 agcaggggcc ggcccaggcg tggcgaaaac caagaaagtt caaaaggccc ggaagggggg
     1441 tcaagaaggc ggaaggatta tgccccgatt tcttttaaca tctccatctc tttgcgcagt
     1501 actgcattaa ctattttttc cagcctgacc cttttctttg cagccttttt actgaaaaaa
     1561 gccttcactt ctttgtcaag ataaacaggc gcttctatct gctctaccgg cctgtaaaat
     1621 ttgccctgta cggctttcga aaaatcatat tctcttttca ttcgtttatc ccctcctttc
     1681 acaatattgt ttctcttcat tctttgtggc ttttcttgcc gatataattc ttatagtctc
     1741 tgtgcctttt gcttttcggt acgtatgcac tactaccaat attttaccac tatcagactg
     1801 gccgatggtt atccaccttt cttctttaga agaatgctct tcatccagga gtgtcaataa
     1861 gcacttatct gcaaagacat agcaggcttc taaaaaagtt attttatgtt tctgctcatt
     1921 cgccttagcc tttttcagat cccacttaaa gttcaattat ttgacctttc aggtgtacaa
     1981 aacttctacg aacattaagt cttttcccat taggatggct tattttaaga cacattttat
     2041 acgaaagtga agtatttttc agctgtcgtc ttcttaaaag actcaatagt ctggaagaat
     2101 acgcgcacat gatataatac gccatgaatc cctattcccg cttaatcccg cgactgaacg
     2161 gcaaagagat agaagaacgg tttgagtatt atctcggtct cctgaaaaag ggcgttgcgg
     2221 gtttcatcgt attcggcggt gaacttgaaa cggtacgtga aggaatcaag aaacttcagg
     2281 gtgcttccca tcagccgctc attatcgcct ccgaccttga gcagggactt ggacagcaaa
     2341 ttgagggagg aacccttttc cctccggcca tggccgcggc ttcagcaata aaaaccgccc
     2401 gaagggatgt tgcttcccag ctactgaaga gactttatac tgcattcgct gcggaggcgc
     2461 ggtacacagg aatcaatacc atccttgcgc ctgtgctgga catcaataca aaccctttga
     2521 atcccatcat cgcgacaagg gcattcggag aagatcctga atccgtctca tttttcgggt
     2581 gtgagatgat aagggttctg caggaaaaca gaatcatgtc ctgcggaaag catttccctg
     2641 gacatgggga cacagagatt gactcccaca taaccctccc tgtcatacgt aaggaactcg
     2701 ccgcgcttgg atatcacgaa ctcataccat tcaagagagc aattcatcaa ggggtgggca
     2761 tggtcatgct gggacatctg agtgttccct ctttggatcc ttcaggaaag cctgcatcca
     2821 tttctgacaa agtggtctca tatctgagaa acaatatgac attcaagggg ttggtgatta
     2881 ccgatgcaat gaatatgggc gcattagggg gatatcatga aaatgaggcc tccctcattg
     2941 cactcaatgc aggggtcgat ctcctccttc atcctactga tcctgataac accgcattat
     3001 tcctacgcca gaagaactac cagccgcatc ccttaacgct gaatctcacc aaatctcatt
     3061 ctaccgttca tttcgattct cgcgagcacg gagaactctc cgacgaaatc agtcgtatgg
     3121 ccatcaccat agagagagaa tatcaaggga agataaaaaa gccgtttgtt cttctcgtgc
     3181 atgacgaaca cattaagaaa ggcgacgtct tcttgaatga aatgaagcac gcctatcccg
     3241 cgattaatta ccgtgcaatc ttccctgaag aggacatacc tctgtccgca tttccggaag
     3301 attgcgattt gatcgtgtgc gtgttttcca agataaaggc atggaaagga acacatgcgg
     3361 tctggttgcg caggactatc caacatcttg caggcagggc aaggcttttc atttccttcg
     3421 ggaatcccta tgtgataaag gggctggaaa acgtcacaaa gatatatgcg ttttgggact
     3481 cagattcagc gcaaaaggct gttgctgaga gaatcattgc aacgtgagta atgccacttc
     3541 cctttgacat tgaacaaaag acctcacaca ggaaaggccc tgaacgcata gcgtccaggg
     3601 ccccgcgtca aaacccggaa gactttttgc taatgtccgc cgggaaagaa ggtatagctc
     3661 acccagacaa gcattcccaa cagggcagct ccaattataa gactgtagct aatgagcttc
     3721 ttctccacag gaagcagcgg ctcatattcc atctttttta attcttctgc taattttggt
     3781 tcctcaggca tacatacctc ctttctctta acctgtcacg atcggcggtt ttaccccatg
     3841 gaagaatatc catgagataa ccagccctat ccagatgata aacccgaaga ggcagaggat
     3901 atacactgcc gccagtttcc cgatgccttc ttcccatagt ttcttaaagt tggaaacaac
     3961 tccaatggta aagaaggtca ggagaaagaa catcaccctg aaagaattac tgccatctgt
     4021 tgccaccttc gcatttgcca gggtcgcctt tggttcttta atctgccctt ccaggccctt
     4081 tatcttgtcc tttgcagcat tgatttgttc agtcagcgct gccagcgcca cggggtctgt
     4141 tgtggtcggg atctgtttct ctgacttggc aacttcctct ttcaaggggc ctatctcctt
     4201 ttcaacaggt agtacagtcc tggtagcagg caaacagacc agcagcagca gtatgaacgt
     4261 tatgacatac cctataacaa acttcgggaa cctgttccat atctcgcctg ccccgacctt
     4321 ctcacccggt ttgcactcaa tcttggcgca ccatatatag gcgagtatga atgcccacac
     4381 cccgataaag atatcgatga acagctttac cgttgtggca gtcatcgtca gccagccttc
     4441 cttgtagttt actccctcca ttgtcagtgc ctttgccctt acgagtgcat cagtaactgc
     4501 accgcttgca actgctgcac catcggtctt taccgcaaga cccatccatg cgccggccac
     4561 cagcggctct ttccataaga aggtctgcgc catgaagggg agaacgataa gctcaataac
     4621 ggcaaagata acgaccagag atgacaccat aaccggcact attggccttg cccttatggc
     4681 tccacctgtc gctatggccg ccgacacccc gcatattgat attcctgatg caaggggggc
     4741 agcccattcc ctgctgaact taaagtattt ccttgcgacg aagtaaacca gggcccaata
     4801 gatgagatac gcctcgacga tggcgcaaag ccccctgaac atcactgcag ttgccagtcc
     4861 gaaggactcg gccgccttca caccgagcag cgcacccatc aggacgatgg cagtcttgat
     4921 ataaagctca ggtcttatta catctttcag gtaagctgag agcttgggga aaaagttgcc
     4981 cacagcgaga cctgcgatga gcgcgacaat aaatcctgac tctcctgtca gtttcagtga
     5041 ccatggaact cccgccttct tggcatctgt tgctgcgatg tatgcatagt taccgataag
     5101 ccagcatgca taactcaccc agaagacaat agtgaaccct ataataaact tgccgatatt
     5161 caccctcaaa gccgcagcac cgaccaacat caccacgagc atgaaaagat aggttaagag
     5221 caaagacgtg aacccggata ttcccgcttc atatgtatcc cccaccttga gtgcagtggt
     5281 atcctttacc gtgatggtct cctcttttcc atccgccttt ttgagggtca ccttctgacc
     5341 atcaatcttt gtaatttctc cctttaccat gacataactt tttgttgtgg cagacgttgc
     5401 ctttgaaacg tcagtccatg tctttgttga tattccccat ccgaggagat cctgtcctgc
     5461 aaaagcaaaa agcgagatga cgaaaataaa aagtccaagc cacagggaca gccaatcctc
     5521 gcttatcccc tgtttttttt cttccatacg tcctcctttc ttcatgagtt aaatgcaggt
     5581 gtgcgtgaca ggcttccact aagtcatagt ttcacctccc agctccaaag gtaacggcac
     5641 agtatgtggt acttctcaca atggtggtgt aaatccatgc tgctctgccc ctttcgttaa
     5701 gagacaccag aagctggttt attgttccat tgtagtgaaa gaaaaaggac aattcaacat
     5761 aaaatatgaa tattgcgcaa atctgtcaca cttcggaagg agacttcttg acattcattt
     5821 gcctttctcg ttaaaataac acgtttttta tagattttct gagtggtttc ttggacacaa
     5881 gggttacgca ttgttccata atcgcatagg cagacggacc tttttaaaag catcccttgc
     5941 aggcgcagca atgctccttt caaaacctgt ttttcctaaa gaccttccca tccacaaatt
     6001 tcctcttggg acgctctccc tttacaacat tcacacggat gaaagattgg aagtgacgta
     6061 tagaaaagca tcaggcgaat atgatcagga ggcgctcaga gccttgaact gggtcatgag
     6121 gtgccattat accggacagg tcgcggatat ggatgtcaac gtgattgaat tcctcaatat
     6181 ggtggacaag caactgggag gaaataacga tatccatgta atatccggat tcaggtccca
     6241 tgaatacaac cagctgctag tagaaagggg cagacacgtg gccagaaaca gcctccatgt
     6301 acagggcaag gccattgata tcagtatccc caaggttgac cttgttctat taaaagatac
     6361 tgccctgaac ctcagatatg gtggtgtcgg ttattaccct cgtgatttcg tccacctgga
     6421 ttccgggaga ttcaggacct ggtaatcatc ctgtagtatt tgccgttttc tctcttccct
     6481 ggttccatcc tttctttcat ctctgtgccc cacttccgcg gggcgactca tagttcatgg
     6541 ctcattgttc gttgctatca gctaaactgc taaccgctgc ctctcaattg atgcgcgcag
     6601 cgcctgtgcc ccaacgttaa aacgcacctc cttcacagat aagaaaagag tgcagatatg
     6661 caagcaactc ctctgatgtc tcatgtggat gaaaatcgga aaaacgtggt atcattcctc
     6721 atggcctaca aggatttgag ggaatttata aagatactcg agaagagagc cctcctcagg
     6781 aggattagcg ttgaggttga tcccatactt gagataggcg aaataaccga caggatgtgc
     6841 aaaagtcccg ggggagggaa agcgctcttt tttgaaaagg tgaagggctc ttcctaccct
     6901 gtggtaacaa acatgttcgg ttccttcgaa aggatgagcc ttgcccttga agttgaaaag
     6961 ctggatgatg tggcgaggcg aatagaagaa ctgctgaacc aggcagcccc aaagacattg
     7021 atggaaaagc tcgcaatgct gccaaagctc tttgaattct ctaagtacct tcccaaagag
     7081 gtaaacaacg gcccatgcca tgagattatc gaaaaggaga atcctgacct gagcaggttt
     7141 cctgtcctca agtgctggcc aggtgacggg cagccgactg atgaggggag attcattacc
     7201 ctccccatgg tcttcaccag agaccctgac tcggggcgtc ctaattgtgg aatgtacagg
     7261 atacacatat acgataagac taccaccggt atgcactggc atattcacaa agatggtgca
     7321 agacattatg accgcttcag atctctcaat aagaggatgc ccgctgccat tgcagtcggc
     7381 agtgaccctt cggtaatcta tgcggcgagc gcccccttac ccgaatccat cgatgagatg
     7441 ctcttcgcag gatttctcag gaaagaaccg gtggagatgg tgaaatgtat cacttctgac
     7501 attgaggttc ccgccaacag cgagcttgtg atcgaaggct atctcgaacc gggtgagatg
     7561 aggattgaag gtccgtttgg cgaccatacg ggattttatt cagcggcaga ttattatccc
     7621 gtattccatg cgacctgcat cacccacaga aaggacatga tctaccctgc taccatagtg
     7681 ggcaagcctc ccatggaagt ggtatttcac aacgcagcat tgatctcaat aaaaaagagt
     7741 taccctggcc acgcaaagaa gattatccat ggcctatggg gcaaaggcca gatgatgttc
     7801 gcaaaactcc tggttattgt agatgatgac gtggatgtcc agaatatctc ttacactgct
     7861 tggcgtgtgc tgaacaatgt ggactggaaa agggatgtag tcattgctga gggtcccctc
     7921 gatgacctcg accattcggc aagctggccc cggtacggat caaagatggg cattgacgcc
     7981 acaagaaaaa ccagggaaga gggcatgatg cgcgaatggc ctcaagaact atcgatgtca
     8041 gaagaaatca aaaagcgtgt ggatgccaga tggagagagt acgggttcga ataagcagaa
     8101 ggagattttt cttgctcccc gataccattt ctcaagagtg attccataat tcgctcagtt
     8161 gtcaaagacc gcgagatttt aattgaactc attcatggcc ttatccacac catctcttaa
     8221 taccatggca atcgcatccg aagcccttac gatagcatct ttgattaagg ggatctgatc
     8281 ttttccgaac tttctgagga cgtactcttc tgcaggaaca ccttcttcgc gtccaacccc
     8341 tattttcacc ctgacgaatt ccctcgtccc tatgctctct attatcgaat caacccccct
     8401 atgtccgccc gaggaacctt ttttccgaat tttcagtttc ccggtctcca tgtcaagatc
     8461 gtcatgcacc acaataaggt tctctggtcc cagaggatat ttcttcatga cctctttcac
     8521 cgcaaagccg cttctgttca tgaacgtcaa gggctccatg aggatcactt tattaccctc
     8581 tatggagccc ttgcctatca tataggactc tctcgtcttc agttctattc catacctgct
     8641 cgcactatca tctgcaacca tgaaaccgac attatgtctg gtttttgagt acctcctgcc
     8701 cggattgcca agacaaacaa taagccacaa ttacgccttt tctcccgctt cttcttcctt
     8761 cttgcccttc ttgatcacct caggctctgc aacctcaggg gctgcggctg cggcagcagg
     8821 cgcagcgacc tcttcaacca caggggccac cacggttgca acaaggtctc cgggatcggt
     8881 gagaatctca atcccctccc caatgggtaa atcacccaca tggatcgcct tgccgatttc
     8941 cagggaagag acatccacct ccacgtgtcc agggatctta tcgggaaggc actgtatttc
     9001 tatctccctc actccgtgct gcaggatacc tccgtccctc ttcaccccga taggttcacc
     9061 tgtggtggtg atatacactg taacacggat ctcctcggta agggagacct cgaagaaatc
     9121 cgtatggagc aactcacctt tcagggggtc aacctgatag tccttcatga gggcaacctt
     9181 gttttcacca cctgcgaact gaaggttcac catgacctgc tcacccgcgg ttgcatggat
     9241 gaagctgatg agctcctttt tatctatctt tatgggaagg gaactgcctc cacggtaaat
     9301 aaccgccggg actgctcctt gtctcctcaa cgacctcgca actccctttc ctgttttgtc
     9361 tcgcgtctct gcgcgaaggg taatcctttc cattctctcc tcctctatcc aaaaagtgaa
     9421 cttatcgaag actcctcatg aatcctcttt atcgcctcgg caagcagagg cgcaatgctc
     9481 aataccgtaa gcttgctgca ctgttctttc ttactgtcga ggggtatggt attgaccaca
     9541 ataacctcct caatgacaga actgttgatc ctgtctagtg caggacctga caaaaccgca
     9601 tgcgttgaag ctgcgaatac cctgtgtgcg ccgtgctcct taagtgcgga tgcggcctgc
     9661 gtcgtcgtgc ctgcagtgtc aatcatatcg tcaaggagca gcgtatcacg gcccttgacg
     9721 tcccctatca cattcattac ctgggataca ttggccagtt ctctcctttt gtccacaatg
     9781 gcgagggagc attgcagccg cttggcaaat gccctcgccc tttcgacccc accggcatcg
     9841 ggtgacacga tcacaaggtc cctcaagttc cttccacgga tataatcgag gagaaccggt
     9901 gaggcgtaga ggtggtccac ggggatgtta aagaacccct gtatctgtcc tgcgtgaagg
     9961 tccaccgcaa gcaccctgtt cgcacccgct gcggtaatga ggtctgcaac aagctttgac
    10021 gatatgggta cccttggctg gacctttctg tcctgccgcg cgtatccata ataggggacc
    10081 actgccgtga tccgccctgc agatgcccgc ttgagggcat caatgatcag aagcagtttc
    10141 atgatgttgt cgttgacagg aggacaggtg gattgaatca caaaggcgtc cgttcccctc
    10201 acattttcat tgatctgaat catgatctca ccgtcgctga aggtagtgat ggtagtatca
    10261 cacatggata tgccgagaca gtccacaact tcctgcacca accccctgtg gctgcttcca
    10321 cttatgacct ttattccttc cggcattttc cctccgacat agcgcgacgc gtaacgcaca
    10381 acgcgattct gtttttactt gtcactattt acttgtgact attcactaca tggctggggc
    10441 gggaggactc gaacctccag atgggagatc caaagtctcc tgacttgcca tttgtcgacg
    10501 ccccaagatc aaaaagcaaa atcaaaaatt ggcacactcc tgaatttcta atgaataagc
    10561 gttcttacta ccctgcacca gtgaggtgac atgtattctc tcgcgttttc ggcatggcgt
    10621 tcggaatcaa acacaccaaa caccgaggac ccactcccgc tcatggagga aaagagggca
    10681 cccctcagca tgagactacg cttaatttca gcgattaccg ggtatctcct caccacaagc
    10741 ggctcaaggt catttccttg cagagatgac aggaacgcaa aatcctctct tccaagagcc
    10801 tgacagaaca gtttaatatt attatgtttt tttgtcaaca cctccctcga ctgagaaacc
    10861 ttgtcatatt cggcgtatgc ccatgcagct gaaatactta tctctggctt tacaagcagg
    10921 aggtgatagg atttcgttaa ttccacggga gacacaacct cacctcttcc ttgcaccaca
    10981 gacacgggac catggaaaaa gaatggcaca tctgaaccca gggcctctcc gagccctgca
    11041 aggtcccggg gtgtgcaatc aagattccag agcctcttga gacctatgag agtggcggca
    11101 gcatcgctgc ttcctccccc gagtccggca gccaaaggga tctccttgtg gagggttatt
    11161 ttgactcctg acacaacccc ggttttctct ctcattagtt tggccgcttt atacacaaga
    11221 ttctcttccc tggggatctc cgcacctgtc aagacctcaa cgcgaggaga attctccatg
    11281 acgagggaat cactgagggt aatacactgc ataaggctca caatatcatg atacccgtct
    11341 ttccttcttc ccaatacatt caagaaccag tttatttttg caggagcgtt tacggtaagc
    11401 atcttagccg tcacatcaag gttctcatat ctgatagctg aataaagtta taagcctgca
    11461 gtatcttata gagttatacg tagccagcgt tatgaggctt ccgatgagag tggactccct
    11521 tattgctcat tcatgcaaca ccaccccgaa ttcagtgcgg agtgttatcc gcctctagga
    11581 cattacctga cctacctatt tcaggccctt taccttctct tcaaccctct ctttcagccc
    11641 ttcttctttc tcgcccattt ccatggactt cctccacgcc ttaaaggcag cgtccttgtt
    11701 acccatggaa gagtacacat caccgagatg ctcatataaa acaggatcgt ccttcacacc
    11761 ctcagttgcc tccagcagat accgaagggc gtcttcgttt ttccccagtt tgaaatacac
    11821 ccaaccgagg ctgtcaagga tatagccgtt gccaggtttt atctctaatg ccttttggat
    11881 gagggaaagg gcttcctcaa gatggattcc cctgtcggca tagctgtaac cgagaaaatt
    11941 gagcgcatca gcattttttg gattctttcc gatcaccttc agaagatagc tttccatctc
    12001 atcaaatctc ttcgttttct catacagtac cgcaaggtta aaattgagat cttcattatc
    12061 accaaaaagc gcaagccctt ctttgaatac ctcttctgcc ttcttgaagt cctttgcctc
    12121 tatacaggtc atgccaaggt agaggtaaat ttcgggctta tccttttcaa acgacagcac
    12181 ttcttcgtat gtcttgatcg cattatcgaa ctgcttaagt ttcgcataca aaagaccgag
    12241 gtgagcaaat gcatcaagat tctttggttc gagcgagatt atttttttta attcatcaac
    12301 cgcctcgggg tatttctcta tctcctccag ggcaatggca agataaaacc tggcagccgt
    12361 atcttcgggt tttgcctcca gaacaagcct cagctcctct attgcgcggt cgtaaagctt
    12421 atcctcaaga tacaggagac ccagtcttaa atggacatcg atgttcgcag ggtcctggtc
    12481 actcagttcc ctgagctggg ctatcgcttt atccacactc ttttgtttga tgaagacctg
    12541 acttaacctc tccttagcac gcatgtcgtg gggattcagt tccgctgcct tgccgtaata
    12601 tgtctctgcc tgggacagat cctcattcat ctcgtggatg accccgaggt taaagtacgc
    12661 cggctcaaag ttcggtttca ggtcaataac cttcttaaag tactcctctg ctttatcata
    12721 cctctttgtc tccatgtaaa tgacccccaa ataataagag gccatcacca ccccagaatc
    12781 ttgcttgttt aatgattcca ggatatttac cgccttgtca tagctctgct gtgaggcata
    12841 aagcacccca aggaaaagat atgcatcgct cacagagggg tctatcttga tcaccttctc
    12901 ataaagggat attgcctcgt ccagcctctt ttggctattg tacatttggg cgagcagcat
    12961 gagggcgggg acataggttc cgtctgccct taccacatct tccattgtcc ttatcgcatc
    13021 ctggaccctg cctgtcctca gatagatata actcatttgt gtcttgagat aatacgacga
    13081 gggatcaagc ctaagcgcct cattatagtg ttttagtgcc tcatcccaat cgcccgaaag
    13141 ttcagcctca tatccaagca tatattcata gtatgcccct gcaggcggct cgggaagtgg
    13201 ctccggagtt ctctgctgat ctttaacccc tggcgaggcg caggaaagcg ttaaggcagc
    13261 aagcgccagt atcagcatac gaacaaagac ggacattatt ttctccatcc tgaattataa
    13321 cagatacctc atgaattaga acagaattta ccttttccct cttaaaacat ccgattaacc
    13381 tccccgtagc ataatcattt accaaaagaa gcatctgaaa aagttacgat acattttcga
    13441 cgcagaagtt tgatggatca ttacgccagg gcatattcta tcttcagttg gatcaagggc
    13501 tggaggaggg tacaaggtat gctgggaagt cttcggcgat ttgatgagag tgagg
//
LOCUS       DATKOU010000188         4077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3723902, whole genome shotgun sequence.
ACCESSION   DATKOU010000188 DATKOU010000000
VERSION     DATKOU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3723902"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VMU21_08775"
     CDS             complement(<1..330)
                     /locus_tag="VMU21_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77656.1"
                     /translation="MKIVLATDGSEHSEAAAQFLSRFHFSARDEIIVLHVVSEIPYDD
                     DYHAQIKHVIKKVAPKILHSTQKLLKPVRAQLSSIEEDGIPDTAIIQVAENSGADLII
                     MGARGLKS"
     gene            complement(440..865)
                     /locus_tag="VMU21_08780"
                     /pseudo
     CDS             complement(440..865)
                     /locus_tag="VMU21_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(997..1134)
                     /locus_tag="VMU21_08785"
     CDS             complement(997..1134)
                     /locus_tag="VMU21_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HUO77657.1"
                     /translation="MLDHLRDDLRKLRNPAKAEILSRFFKTVRGEYGEGDVFLGIPVP
                     Q"
     gene            complement(1152..2138)
                     /locus_tag="VMU21_08790"
     CDS             complement(1152..2138)
                     /locus_tag="VMU21_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HUO77658.1"
                     /translation="MPANLPPEYFGAEKRFKEVATSQEKIRALEDLISTVPKHKGTDK
                     LRADLRKKLSQLREEALRKKKSGKGDLYTIEKQGAAQIALVGFPNSGKSSLLKTLTNA
                     NPVIADYPMSTLTPLSGMMHFEDIQFQIVDLPPIGNESTDGWVSGILRIADILMLVID
                     LSEDPLSQADLLMVHLSEWKIPLLKKGERRDEEKGSPSKPLVLVGNKFELSGAEDGMK
                     TVEQKYQSLFPVAGISSKNKQGIEELRRAVFENSRIIRVYSKEPGKEPDLSTPFTLPA
                     GSSVIRLAELIHKDFVRNLKYACIWGSAKFAGQRVQKDYILHDRDVVEFHVR"
     gene            complement(2367..3488)
                     /locus_tag="VMU21_08795"
     CDS             complement(2367..3488)
                     /locus_tag="VMU21_08795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUO77659.1"
                     /translation="MSKKLFTFNEMNQIKLVKFIDKMIGKTLVFLLSEKNLAQEGVSK
                     KILDILFIRPGGIGDAVLLVPAIEAVRRKFPDARIDILCEKRNVDIFGLTKAVNKVYV
                     YDRGLELLQCLRNNYDVVIDTEQWHRLSAVVAYLTKAPKKIGFSTNERRNVFTHQIPY
                     SHDDYEVYSFFHLVEPLIGNGIGFNVNEPFLNITDDIPSPLLAWFKERKDKVVALFPG
                     ASVKERRWGGTRYGKVGKALGEKGYAIVILGSIKEREEAEEIKASVGDHLDLTGEIRL
                     RDIPSVLRRCRALITADSGLLHIAYAVGTPTVSLFGSGIEKKWAPCGDRHIVLNKHLD
                     CSPCTRFGYTPKCRRNVECLALIHVDEVIEAARRTLDAS"
     gene            complement(3455..>4077)
                     /locus_tag="VMU21_08800"
     CDS             complement(3455..>4077)
                     /locus_tag="VMU21_08800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO77660.1"
                     /translation="IAASYVKSGKVLDFGCGTGHFIKRFREAYETWAYDISYNALDST
                     REVAPNARICNSLPSLLQNRFDLVVSIHVLEHLTDPIETLAMFSTILNDAGVLMYVVP
                     DISGIGQRLKKQKWFAYGDLSHVSLYPAEKWLRWTESAGFKILKSGTDGLWDVPYLPV
                     VPVWIQKLLFYPLPALQVITGRLFLPVGWGESLIVVCRKNCSRSMK"
BASE COUNT         1057 a          995 c          922 g         1103 t
ORIGIN      
        1 actcttcaat ccccttgctc ccatgatgat gaggtctgcg ccggaatttt ctgccacctg
       61 tatgatagca gtgtccggta tcccatcttc ttcaatggag ctgagctgtg ccctcaccgg
      121 tttcagaagt ttttgtgttg aatgaagaat ctttggggca accttcttaa tcacatgttt
      181 gatctgggcg tggtaatcat catcataggg gatttcggaa accacatgga gaacaataat
      241 ctcgtcgcgg gcagaaaaat gaaaacgaga caggaattgt gcagcagcct ctgaatgttc
      301 cgatccgtcc gtggccaata ctatcttcat cttcttgcct ttccggtcag tgttccatct
      361 caatcttagc acatattcaa gccacttaca aaaaggatat gcaggaaaaa ggacgtttga
      421 ggacagggga aagtagaaac tattttttca gataggcatt ccttgtcgtt tcatcgaatc
      481 gctcaatggc gtatctcagc atagtccttg gcatgttacg ggaatgtgtt ctcagaaatt
      541 gttcttccgt tcttctgtcc cttttgccga tctcccgcaa catccatccc accgctttgt
      601 gaatgaggtc atttgtatcc ttcaggagta attcggaaat gtgcagggtg tctttaaatt
      661 cattcctcct gataaaggca aaggttgcca taatggcgat cctcctctcc cacaggttat
      721 cagatcccgc aaggcgagag aggagtgccc tgtctttgtc caggaggtaa tcacccagga
      781 tattccctgc ggaaatatcc acaagatccc aactgttgat ccgccttgtg ttccttaaat
      841 agaaatcaac tactgcccgc ctgtccgtgt tctctgcctt atgtacttcg tgatcaggat
      901 aagaagggca acgagcctct cttcatgtat cctgctcgaa agcagcattc ggagagcacc
      961 caggtgaaag tcgcaatact tttttgcaat cctcctctat tgaggcacgg gaatcccgag
     1021 gaaaacatca ccctctccat actctcccct cactgtcttg aagaaccggg agaggatttc
     1081 tgcctttgcc ggattcctga gctttcgtaa gtcatccctc aaatgatcta acattgtctc
     1141 ttgcgggtgc ttcatcttac atgaaattcg accacatccc tgtcatgaag gatataatcc
     1201 ttctgcaccc tctgcccggc gaattttgcg gacccccata tacaggcata tttgaggttc
     1261 ctgacaaaat ccttatggat caactcagca agccgaatga cagaagagcc agcggggagg
     1321 gtgaagggag tactcagatc gggctcctta ccaggctcct ttgaatatac ccttataata
     1381 cgggagttct caaaaactgc gcgccgtaat tcctcaatgc cttgcttgtt ttttgaggaa
     1441 atccctgcaa ccgggaatag tgactggtat ttctgttcaa ccgttttcat tccatcttcc
     1501 gcgccagaaa gctcgaactt gtttccaacc agaaccaagg gcttggatgg ggaccctttc
     1561 tcttcgtccc tgcgttcccc ttttttcaga aggggtatct tccattctga caagtgtacc
     1621 atcagtagat cggcctggct caggggatcc tcagagaggt caatgaccag catgagaatg
     1681 tcagcaattc ttaggatgcc ggaaacccag ccatcggtgg attcatttcc aattggtggg
     1741 aggtccacaa tctgaaactg gatgtcttcg aaatgcatca tcccagagag gggggtgagg
     1801 gttgacatag ggtaatcggc gatcacagga tttgcattgg tgagggtctt aaggagagat
     1861 gacttccctg aattggggaa tcccacaagg gcgatctggg cagcaccctg cttttcgatg
     1921 gtatagaggt ctcccttccc tgacttcttt ttcctgagag cctcttctct tagctgggag
     1981 agctttttcc tcaggtctgc ccggagtttg tccgtacctt tgtgtttcgg aaccgtggag
     2041 attaagtctt caagtgccct tatcttttcc tgagaggtgg cgacctcttt aaagcgcttt
     2101 tcggccccga aatattctgg agggagattc gccggcatac taagaatata ccataaggga
     2161 ggtatgctga aagagtacct tatcccggaa atcagaacaa atgtgcgaaa gatccttgat
     2221 tctatcgcaa gaacaacgtc atcaaacgat aagccaacaa aaggatacac atgaagaaaa
     2281 cggcagccac agttgagaag aggggagtga aatgttgagt cctctggccc cttttgttaa
     2341 ggggttggtt cctaatgaaa agaaacttag gatgcatcca gagtcctcct cgccgcctca
     2401 attacctcgt caacatgtat taaggcgaga cattcaacat ttcttctgca ctttggcgtg
     2461 tacccaaatc ttgtgcaagg actgcagtca agatgcttat tgagcacgat gtgccgatcc
     2521 ccgcagggag cccacttttt ctctatacct gaaccaaaga gagaaaccgt aggggtaccg
     2581 actgcataag cgatgtggag caaaccagaa tctgcagtaa ttaatgccct gcatcttctc
     2641 aggacagaag gaatgtcacg gaggcgtatc tctccggtaa ggtcaagatg atcccccaca
     2701 gatgctttga tctcttcagc ctcctcgcgt tcctttattg agccaagaat aactattgca
     2761 tatccttttt caccgagcgc cttccctact tttccatacc tagtccctcc ccatcttctt
     2821 tcctttacgg atgccccagg gaaaagagcg actaccttgt cttttctctc cttaaaccag
     2881 gcaagaaggg gtgaaggaat gtcatcagta atgttaagaa aaggttcgtt gacattgaat
     2941 ccaatgccat tgcctatcag cggttctacg agatggaaga agctgtatac ctcgtaatcg
     3001 tcatggctgt aagggatctg atgggtaaat acgttccgcc tctcattggt ggaaaaccct
     3061 atctttttgg gagccttagt gagatacgca accactgccg agagcctgtg ccattgctcg
     3121 gtatcaataa caacatcgta gttgttcctc agacactgga gaagttctag ccccctgtca
     3181 tacacgtaaa ccttgttcac cgctttcgta agtccgaaga tatcaacgtt ccgcttttca
     3241 cagaggatat cgatgcgggc atcgggaaac ttccttctca cagcctcgat agccgggaca
     3301 aggagcactg catccccgat gccaccggga cggataaaga gaatgtcgag tatcttttta
     3361 gagactccct cttgagctaa attcttctca gaaagcagaa agactagtgt cttgccgatc
     3421 attttgtcga taaactttac gagtttgatc tgattcattt cattgaacgt gaacaatttt
     3481 ttcgacatac cacaataaga gactcccccc agccgaccgg tagaaaaaga cgtccggtaa
     3541 taacctgcag ggcaggcaac ggatagaaga gcagcttctg gatccaaacg ggcacgaccg
     3601 gtagataagg aacgtcccat aatccatctg ttccgctttt taggatttta aatccggcag
     3661 attcagtcca acgcagccat ttttcggcag gataaagtga gacgtgagag aggtctccat
     3721 aagcaaacca tttttgtttt tttaagcgtt gtccgatacc tgatatatca ggcacaacgt
     3781 acattaatac accagcatca ttgagaatcg ttgaaaacat tgcaagtgtt tcaatcgggt
     3841 cggttagatg ttcaagaaca tgtatggaga caacaagatc aaacctgttt tgcagtaaag
     3901 aagggaggga attacaaatg cgagcattgg gagcgacctc ccttgttgaa tcaagagcat
     3961 tgtacgaaat atcatatgcc catgtttcgt atgcttctct aaatcgcttt atgaagtggc
     4021 ccgtaccgca gccaaaatcc agtaccttgc ctgatttaac ataagatgct gcaattc
//
LOCUS       DATKOU010000189         5333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1037390, whole genome shotgun sequence.
ACCESSION   DATKOU010000189 DATKOU010000000
VERSION     DATKOU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037390"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..607
                     /gene="raiA"
                     /locus_tag="VMU21_08805"
     CDS             83..607
                     /gene="raiA"
                     /locus_tag="VMU21_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HUO77661.1"
                     /translation="MNIIVNGRHLEITPALRSYAEEKLKKFDRYLSHITEATVTLSIE
                     KYRHKAEVLLKANGMLIQAEGITGEIYSAIDEVVEKLEKQVKKYKEKLVSHRKGEGKS
                     APDVIPASPEPEQGRIIKKKRFDMKPMSPDEAAMQMELLDKAFFVFSNHTTGEINVIY
                     RRNDGNFGLIEPAK"
     gene            695..1564
                     /gene="rapZ"
                     /locus_tag="VMU21_08810"
     CDS             695..1564
                     /gene="rapZ"
                     /locus_tag="VMU21_08810"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HUO77662.1"
                     /translation="MSKNMKPMVVIITGLSGSGKSVALRALEDEGFFCVDNLPVTLME
                     SFLSIVSSNGHNTRIGIGVDIREKEFLASFDSVITALRVNYSVQIVFLEADKDALIRR
                     FKETRRPHPLVSPEVSVEAAIDAEHRMLLPLRSASDRVVDTSSYTPHQLRHLIASLFK
                     TTASPDTMSINLISFGFKFGIPQNVDLLLDVRFLPNPNFVPALKAHKGTDREVKDFVL
                     EQPATKDFLERTAALLNFLIPRYVEEGKAYLTIGFGCTGGRHRSPAIVENIAGYIRQT
                     HGIEPAVTHRDME"
     gene            1581..2708
                     /locus_tag="VMU21_08815"
     CDS             1581..2708
                     /locus_tag="VMU21_08815"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HUO77663.1"
                     /translation="MIYLDHNASTPVDPEVADAVHSVLRTNFGNPSSGHDIGLRAKAS
                     MEKARLQVAELIGASPPEVVFTSGGTEANNLAIIGTALKKKKGHIITSLIEHPSVMNP
                     CRHLEAMGFGCTYVGVNHEGRVALEDIRKAIRKDTFLITIMHANNETGVFQPVEEIGA
                     LAREKGISIHTDAAQTVGKVPVNVEKLNVDMLTVATHKFYGPKGIGALYMRKGIELNP
                     LLFGAGHERGLKPGTENVPGIAGLGKACEMAGIYMKDRVSHTKKLTTMLCNGLMGRIS
                     GLRINGHETLRLPNTLNVCIPGVNSTDLLEMIKNNVAASSGSACHAGENKPSAVLMAM
                     GISEADAMSSIRLSTGKDTTEEEIRESVEIIDKAVQALIRS"
     gene            2813..3145
                     /gene="trxA"
                     /locus_tag="VMU21_08820"
     CDS             2813..3145
                     /gene="trxA"
                     /locus_tag="VMU21_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HUO77664.1"
                     /translation="MSEEIITINGNTWEGQVSRSRGIVLVYFWAPWCGHCTRYAPTFE
                     ELAREYAGKITFAKLNCDENPDVASKCQIKGTPTLMIYRDGEQIDSIIGGIPKVQLQA
                     KLDSLLKG"
     gene            complement(3369..4616)
                     /locus_tag="VMU21_08825"
     CDS             complement(3369..4616)
                     /locus_tag="VMU21_08825"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUO77665.1"
                     /translation="MFKKYRLDNGIPLVTERIRNVHSVALGIWVKVGSRYELPEKNGI
                     SHFLEHMFFKGTRKRTAKDIAVDIDSIGGDLNAFTSRETTTFYVKVLDEHLHRGIDLL
                     TDIFLHSTFPETDMEKEKSIIKEEIKMVEDTPDDYIHDLFYLSVWGEEGIGQPILGRR
                     DTIKSFASEDLMSHVRRYYGTKDTVIACAGNFEPEKLLHELNHILGGLRRGSEPKTGN
                     APLFKPVTKVYSKDLSEVHSCIGVKGIAQGSGERYALYLLNAILGAGVSSRLFQEVRE
                     KRGLAYSIYSFIASYFDTGIWGVYAGSAKKRVSEVIEIIVKEMRELKDTITHDEFTRA
                     KDQLKGNLVLGLESTNNRMQNIARQEIYHERYFSPEEIIREIDAVTLREVKELSEKLI
                     GQGSIALTVLGPVQPSQLTSLSL"
     gene            complement(4715..>5333)
                     /locus_tag="VMU21_08830"
     CDS             complement(4715..>5333)
                     /locus_tag="VMU21_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUO77666.1"
                     /translation="TGTEAGITAFQMDLKTGGVSKGVMEKALEQARQGRVFIIGKLKE
                     ALSAPRENLAPHAPRIYTMQIKQDKIRDVIGTGGKVIRGIIEQTGVKIDINDSGIINI
                     ASSDETSASKAMDIIKGIIAEAELGKIYHGKVKRVVDFGAFVEILPGTEGLVHISQIS
                     EKRIAKVTDEIQEGDEVLVKVIEIDKTGRIRLSRKEAMREQADQK"
BASE COUNT         1365 a         1359 c         1293 g         1316 t
ORIGIN      
        1 ataccgtgaa gagcttaaga ttccccccca gggccagaga aagagccatg actaaccata
       61 acacattcgc aaaggaggag ctatgaacat cattgtaaac ggcagacacc ttgaaattac
      121 tccggcactc agaagttatg cagaggaaaa actgaagaaa tttgaccggt acctctcgca
      181 tattaccgag gcgaccgtta ctctttccat agagaagtac cggcataagg cagaggtgct
      241 cctcaaagca aacggaatgc tcattcaggc cgaggggatc acgggagaga tctattccgc
      301 cattgacgag gtggtcgaaa aactcgaaaa acaggtgaag aaatacaagg agaaactggt
      361 ctcccataga aagggcgaag ggaaaagcgc ccctgatgtt attcctgcca gcccagaacc
      421 ggaacaggga aggatcatca aaaagaaacg atttgatatg aaacccatga gtccggacga
      481 ggccgccatg cagatggagc ttctggataa ggcattcttc gtcttctcaa accacaccac
      541 cggagagatt aatgtgattt acaggagaaa cgatggtaac tttggactca tagagcctgc
      601 aaaataaaac atcgcgggtt cttcccagac acttctgtca ggagaacccg aacccctttt
      661 acccttctgc taaaataggg atgagggttt tagtatgagc aagaatatga aaccgatggt
      721 agtgatcata acaggactct ctggttcggg caaatcggtt gccctgaggg cactggaaga
      781 cgaagggttc ttctgcgtgg acaacctgcc tgtgaccctc atggaatcgt ttctctccat
      841 cgtctcctcc aacggacaca acacaaggat agggatagga gtggatatca gggaaaagga
      901 gttccttgct tcctttgact cggtcattac ggcattaaga gtgaactatt cggtacagat
      961 tgtattcctc gaggcagaca aggacgctct catacggaga ttcaaggaga cgagaagacc
     1021 tcatcctctc gtttcgccgg aggtttcggt ggaagccgcc attgacgcag agcaccgcat
     1081 gctccttcct ctgaggagtg catcggacag ggtcgtcgat acttcctcct acacccctca
     1141 ccagctgagg catctcatcg cctcactctt taagaccact gcttcaccag acacaatgtc
     1201 gataaatctc atctccttcg gattcaagtt cgggataccc cagaacgtag acctgctcct
     1261 ggacgtgagg tttctgccga acccgaattt tgttcccgca ctcaaggccc ataagggcac
     1321 ggacagggag gtcaaggact ttgtgctgga acagcccgcc acaaaggatt tcctggaaag
     1381 aactgccgca ttgctgaatt ttctgatccc ccgctacgtg gaagagggaa aggcatacct
     1441 gaccattggc tttggctgca ccgggggaag gcaccgttca cccgcaatcg tggagaatat
     1501 cgccggatac atccgccaga cccacggcat agagcccgct gtcacccata gggatatgga
     1561 atgaagggaa caggattgcc atgatttacc tggaccataa cgcctcaacc cctgttgatc
     1621 ccgaggttgc tgatgccgtg cattcggttc tcaggacgaa cttcgggaat ccctcaagcg
     1681 gacatgacat tggcctgagg gccaaggcat caatggaaaa ggccaggctg caggtcgcag
     1741 aactcatcgg cgcttcccct cctgaagtgg tcttcacttc aggaggcaca gaagccaaca
     1801 accttgccat aataggaacg gctttaaaga agaagaaagg ccacatcatt acctccctta
     1861 tcgaacatcc gtcagtaatg aacccctgca ggcaccttga ggcaatgggg tttgggtgca
     1921 cttatgtggg ggttaaccat gagggcaggg ttgccctgga agatatccgg aaggcaataa
     1981 ggaaagatac ctttctcatt accataatgc atgcgaacaa cgagacagga gtattccagc
     2041 ccgtagagga gataggggca ctggcacggg agaagggtat ctccattcat acggatgctg
     2101 cccagactgt gggaaaagtc cctgtgaatg ttgagaagct gaacgtggat atgctcactg
     2161 tcgcaacgca taagttttac ggtccaaagg gcatcggcgc gctttatatg agaaaaggaa
     2221 tcgaacttaa tcctctcctt tttggtgccg gccatgaacg tggtctcaag cctggcacag
     2281 agaatgtccc cggcattgcg ggtctgggaa aggcgtgcga gatggccggt atctatatga
     2341 aggacagggt ttctcacaca aagaaactca ccactatgct ttgcaatggg ctcatgggaa
     2401 ggatttcggg cctaaggatc aatggccatg aaaccctacg gttgcccaat acattgaatg
     2461 tgtgcatacc cggagttaat tctactgacc tcctagagat gataaagaac aatgtggccg
     2521 catcttcggg ctctgcgtgc catgcgggag agaacaagcc ttctgctgta ctcatggcaa
     2581 tgggaatctc ggaagctgac gccatgtcat ccataagact gagcacagga aaggatacta
     2641 cagaagagga gatcagagag tcggtggaga taatcgataa ggcagtgcag gcgctcatcc
     2701 gctcgtaacc ttcggagcgc gtgagtcctg gctgttaaat aagcctgttt cttttgggtc
     2761 acatatggca caacatgagc cctaaaaact attccagtca gaaggaggtc cgatgtcaga
     2821 agaaattatc acaattaacg gcaatacatg ggaaggacag gtttcacgtt cccgtggaat
     2881 cgtcctcgta tatttctggg ctccgtggtg cggccattgc acgcgatatg cccccacatt
     2941 tgaagagctc gcaagggaat acgcaggaaa gataacattc gcaaaactga actgtgacga
     3001 aaatcctgat gtggcgagca aatgccagat aaaaggcacc cctaccctca tgatttacag
     3061 ggatggtgag cagattgaca gcatcattgg cggaatcccg aaagtgcagc tccaggcaaa
     3121 actggacagc ctgctcaagg ggtgagcctt gtctgttcat cataatggta cccggggaat
     3181 cggaactcgt tgttaaagac ctacccaact aaatcagcag cgataattgc attcctaata
     3241 tatattttgt tttttttctg atcaattctt gcagagttgg tttactttag attttcctgc
     3301 atatttatag tgatacttta tataatagcc ataataactt gataataagc atatttataa
     3361 gataataatt aaaggctaag tgaagtaagc tgggagggct gtacagggcc tagaacagta
     3421 agtgcgatgg aaccctgccc tataagtttc tctgaaagct cttttacctc ccgcagggta
     3481 actgcatcga tctccctgat gatctcctct ggagaaaaat accgttcatg gtagatctcc
     3541 tgccttgcaa tgttctgcat cctgttgttg gtggactcaa gaccgagaac aagattgccc
     3601 ttcagctggt cctttgctct tgtgaactcg tcatgggtaa tagtatcctt cagttccctc
     3661 atctctttca caataatttc gatgacctcc gacaccctct tcttggcgct ccccgcatac
     3721 acaccccaga tccctgtatc aaaatacgag gcgataaaag aatagatgga gtatgcaagc
     3781 cccctctttt ccctcacctc ctggaacagg cgtgagctga cccctgcgcc gagtattgcg
     3841 ttgaggagat agagtgcgta gcgctcgcca cttccctgag caattccctt caccccgatg
     3901 cagctgtgca cctcggagag gtccttactg taaaccttgg ttacgggttt aaacagcggg
     3961 gcattgcctg ttttcggctc agagcctctc ctcaagcctc ccaggatatg gttgagctca
     4021 tggagcagct tctccggctc aaagttccct gcgcatgcga tgaccgtatc ctttgtaccg
     4081 tagtacctcc tcacatggga catgaggtct tcgctcgcaa aggactttat ggtatccctt
     4141 ctgcccaaaa ttggctgacc aatcccctct tctccccaga ccgagagata aaaaaggtca
     4201 tggatgtaat cgtcgggagt gtcctcaacc atctttatct cttccttgat gatgctcttc
     4261 tctttctcca tatccgtttc ggggaatgta gagtggagaa agatatcggt gagcagatca
     4321 atccccctgt ggaggtgttc gtcaaggacc tttacgtaaa aggtagtagt ttcgcgggag
     4381 gtaaaggcat tgaggtcgcc cccgatggaa tcgatatcca ctgcgatatc ctttgcagtc
     4441 cgttttctgg ttcctttgaa aaacatatgc tcaaggaagt gtgatatgcc gttcttttcc
     4501 ggcagttcat acctggaacc aaccttcacc catatcccca gggccacgga atgcacattc
     4561 cgtatccgtt cagtgaccag agggattccg ttatcgagac ggtatttctt gaacatatcc
     4621 tcctcatagc aaaaaaggtt aaggctgagg ttaagactga gaagaatctc agccttgacc
     4681 ctgaccttaa ccttttacga tgtaactgtt atgactattt ctgatcggcc tgttccctca
     4741 tggcctcttt cctgctcagg cggatccttc cggtcttgtc gatctctatc accttcacaa
     4801 gcacctcgtc tccctcctgt atctcgtcag tgaccttggc aatccttttt tccgagatct
     4861 gcgagatgtg gaccagtccc tctgtgccag gcagaatctc cacaaaggcc ccgaaatcca
     4921 cgaccctttt gaccttgccg tgatatatct ttcccagctc tgcctctgca atgatcccct
     4981 taatgatatc catggccttt gaagcagaag tttcatcgga tgaggcgata ttgataatcc
     5041 ctgaatcatt gatgtctatc ttcactcctg tctgctctat gatgccgcgg attacctttc
     5101 ccccggtacc gatcacgtcc cttatcttgt cctgttttat ctgcatggtg tatatccttg
     5161 gtgcatgggg ggcgagattt tccctgggtg cggagagtgc ttctttcagc ttgcctataa
     5221 tgaacaccct cccctgtctt gcctgttcaa gggccttttc catcactccc ttgcttactc
     5281 cgccggtctt gagatccatt tggaatgcag tgatcccggc ctcggtgccg gtc
//
LOCUS       DATKOU010000190         3498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_263123, whole genome shotgun sequence.
ACCESSION   DATKOU010000190 DATKOU010000000
VERSION     DATKOU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263123"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VMU21_08835"
     CDS             <1..865
                     /locus_tag="VMU21_08835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUO77667.1"
                     /translation="LKTVLFERGLLGGQIAISKDVENYPGVEGITGFDLAERMVHHAL
                     SFDLSVIQQEVAGIDAGPDLHSVRLANGDTFHTVALILAAGGSVRKLGIPGEAEYLAS
                     GVSYCATCDGFFFRDKTVVVVGGGDTAVEEALYLAKLTRKVYLVHRKNALRASKILQD
                     RLMAESKIEIIWNTVITEIKGDGKTVNAVSFENTETGEKAAIFADGIFIFIGYSPNNA
                     LIPSGVRMNEQGFVITDEKCATSVPGIFAVGDLRQKFANQIVVAAADGCIAALAAAHY
                     VEMKKAEAQAS"
     gene            complement(977..1609)
                     /locus_tag="VMU21_08840"
     CDS             complement(977..1609)
                     /locus_tag="VMU21_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HUO77668.1"
                     /translation="MELIPIKIENGEERAWELVCGLSREDVCRRTGTVFDEKAEAYLL
                     RSFGIDFLVNPCEMGISCPSGRGSLFLGKLRDFFRLSVLWYMSNAKDIPPTGRLIRPI
                     DVKGGHRFSAGTHVLPVDIIAERFARDKEGFIRKGHEYGAEVLSGFGDASIRFYPLPR
                     VPVSLILWLEDEEFTPRVDLFFDSTCEFQLALSDIVWAVAMMCCVVMIDG"
     gene            1714..2412
                     /locus_tag="VMU21_08845"
     CDS             1714..2412
                     /locus_tag="VMU21_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77669.1"
                     /translation="MPEKYRKIITTQNIGTFLELGESFLNRSDFKIVRADSNEEALAI
                     HRTEKADLIIADLDANVMRGELLCATIRESQGLHRVSLIIIHSGAASDILRISHCRAN
                     AFIEQSADPAMLLDTVHQLMNIPVRETYRAPIGVRVQCSSGIRHSMGYSENISVTGML
                     FDSEETLLKGDMISCWFVLPDSTHVRTDAEIVRVADKASEHDTNQYGIRFLDLAAGCR
                     AAIDSYVRRRLRNP"
     gene            2537..2767
                     /locus_tag="VMU21_08850"
     CDS             2537..2767
                     /locus_tag="VMU21_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77670.1"
                     /translation="MASILGKAENILISLDKAREILIMEHAIDIIYSEKHTVRFSTKD
                     AEGIDHHVSDEELRKLKTACENQGFHRIKIEM"
     gene            complement(2780..3196)
                     /locus_tag="VMU21_08855"
     CDS             complement(2780..3196)
                     /locus_tag="VMU21_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HUO77671.1"
                     /translation="MSIFSVSPAKEKDLKVKMDALNIHEEDIEESFVRSGGKGGQKVN
                     KTSTCVYLKHIPTGTEVKCQEERSQSLNRYRARVILVKKVDELTKGKESQEQQRIERI
                     RRQKRKRSRRAKEKMLAEKRITSEKKKFRSSVPAAD"
BASE COUNT          878 a          871 c          912 g          837 t
ORIGIN      
        1 gctgaagacg gtcctctttg agaggggttt gctgggaggc cagattgcca tatcaaagga
       61 cgtggagaac taccctggag tggagggaat tacaggattt gatctggcag agagaatggt
      121 ccatcacgcc ctgtcgtttg acctgtccgt tattcagcaa gaggttgcgg ggatagacgc
      181 agggccagac ctgcactcag tccgcctcgc aaacggcgat acatttcata ccgttgccct
      241 cattctcgca gcgggcggtt ctgtacgaaa gctcggcatc cccggcgagg cggagtatct
      301 cgcaagcggt gtttcctact gcgcgacctg cgacgggttc ttctttagag acaagactgt
      361 ggttgtggtg ggcggaggcg atacggcggt ggaggaggcg ttgtatttgg caaaactcac
      421 caggaaggtg tacctcgttc acaggaaaaa tgcgctgcgg gcaagcaaga tactccagga
      481 ccgcctcatg gcagaatcca agatcgaaat aatctggaac actgttatca ccgaaatcaa
      541 gggggacggg aaaacggtga atgccgtttc tttcgaaaat accgaaacag gggaaaaggc
      601 ggcaatcttt gctgacggca ttttcatttt tatagggtat tccccgaaca atgcattgat
      661 tccctccggg gtaagaatga atgagcaggg ctttgtaatt actgatgaga agtgtgccac
      721 gagcgttccg ggcatcttcg cggttgggga cctgagacag aagtttgcga accagatcgt
      781 ggttgctgcc gcggatgggt gcattgctgc cctggctgca gcgcattatg tggagatgaa
      841 gaaagcggaa gcccaggcgt cctgaatatc gggtgggtag tcaggaacct ccggaggtaa
      901 aaacatctat gaacctcaca gctgtgctca aaatttatgc aagggaacac ggcgcgtcac
      961 ttttcacgct ttctcgtcag ccgtcgatca tcactacgca gcacatcatg gccactgccc
     1021 agactatgtc cgaaagggca agctgaaact cgcaggtgga atcaaagaaa agatctaccc
     1081 ggggcgtgaa ctcctcatct tccagccaga ggattaggga gaccggaacc cggggaagcg
     1141 gatagaagcg gatagaggcg tcgccaaagc cggagaggac ctctgcgccg tactcatgtc
     1201 ctttgcggat gaacccctct ttgtccctgg cgaatctctc cgcgattatg tcaaccggca
     1261 gcacatgggt gcctgcggag aacctgtggc cgcccttcac atcaatgggc cgtataagcc
     1321 ttccggtggg tggaatgtcc ttggcattgg acatgtacca gagcaccgag agcctgaaaa
     1381 aatccctgag ctttccaagg aagagagagc cacgtcctga agggcaggag atccccatct
     1441 cgcaggggtt tacgagaaag tcgatgccaa aggagcgaag gagataggcc tcggcctttt
     1501 cgtcaaagac cgtccctgtc cgcctgcaca cgtcttcgcg ggaaaggccg cagaccagtt
     1561 cccatgccct ttcctctccg ttttcaatct taatggggat cagctccatg ccctattata
     1621 atataacttc ccgtagagcg cgcaacagtg accccggcac acggtactgg aaaaataacc
     1681 gtcttgccaa taaaatagac taaagggact cccatgccgg aaaaatacag gaaaatcatc
     1741 actacccaaa acattgggac cttcctcgaa ctgggagaga gtttcctgaa ccgttctgac
     1801 ttcaaaatag ttcgtgctga ctcaaacgaa gaggcactcg ccatccacag gacagagaag
     1861 gccgacctca ttatcgccga tcttgatgcg aacgtcatga gaggtgaact gctctgcgcc
     1921 accattagag aaagtcaggg ccttcacagg gtctctctga tcatcatcca ctccggcgcc
     1981 gcttcggata tcctgcggat ttctcactgt agggccaatg ccttcataga acaatctgcg
     2041 gatcctgcaa tgctccttga tacagtgcat cagcttatga atattccggt gagggagacc
     2101 taccgggccc ctatcggcgt cagggttcag tgcagcagcg gaataagaca ttctatgggc
     2161 tactccgaga atatcagcgt cacgggcatg ctctttgatt ccgaagaaac actcctgaaa
     2221 ggtgatatga tatcgtgctg gttcgtgctt cctgactcaa cgcatgtgcg cacggatgcg
     2281 gagatagtcc gggttgcgga taaggcctcg gagcacgata ccaaccagta tggcatcagg
     2341 ttccttgacc ttgccgccgg atgcagggcc gccattgact catatgtgag aagaagactc
     2401 cggaacccat gagctgctca tcttccaggg atgtggtact atggctctgc gggactatcc
     2461 tggttccgtg ctttctgggc ttatacggga atgactcaga tttgcgggtt atgaaacaga
     2521 caaggggagg ttttaaatgg caagtatcct aggtaaagca gaaaatattc tcatcagcct
     2581 cgataaagcg agggaaattt tgattatgga gcatgcgatt gatatcattt attctgaaaa
     2641 acatactgtc cgtttcagta cgaaagacgc ggaaggcata gatcatcatg tctcggatga
     2701 agaattaaga aaactaaaaa ctgcctgtga aaatcaaggc tttcacagaa ttaaaataga
     2761 gatgtaacag gatgtgtcac taatcagcag cagggacaga cgagcggaat tttttctttt
     2821 cggaggtgat cctcttttcc gccagcatct tttcctttgc cctcctggag cgtttcctct
     2881 tctggcgtct tatcctctca attcgctgct gctcctgact ttccttgccc tttgtcaatt
     2941 cgtccacctt tttcaccagg ataaccctcg ccctataccg gttcagggac tgtgaacgct
     3001 cctcctggca cttcacctct gtgccggtag ggatgtgctt cagatagaca caggtggagg
     3061 ttttgttcac cttctgccct ccttttcccc ctgaacggac gaaggactcc tctatatcct
     3121 cttcatggat attcagggca tccatcttga ccttgagatc cttttccttt gccggactga
     3181 cagaaaatat gctcatacag tacattttac catgtagtaa attatcttgg acctgtccgg
     3241 ttggcgtggc cgattactgt tctcagagag actgagaaaa tagtccttga tgaagcgcta
     3301 taggaatcct tttccctttc gaataaatga ctaagcaact gttttcattc gcatactcag
     3361 gcttgccatc ccccagtttt gacgctgggc gaaatctaaa gattcggcga tagcaaaaaa
     3421 gatcttgaca gatgaaagat tctcctgtat cttacttgta gtatagcaag taagaggagg
     3481 aggtgtggct aagaaaac
//
LOCUS       DATKOU010000191        12330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3726642, whole genome shotgun sequence.
ACCESSION   DATKOU010000191 DATKOU010000000
VERSION     DATKOU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12330
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3726642"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VMU21_08860"
     CDS             <1..118
                     /locus_tag="VMU21_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77672.1"
                     /translation="ISVVVELIGPIAMEKELRFAIREGGRTVGAGVVTEVLE"
     gene            126..278
                     /gene="rpmG"
                     /locus_tag="VMU21_08865"
     CDS             126..278
                     /gene="rpmG"
                     /locus_tag="VMU21_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HUO77673.1"
                     /translation="MRDIILFQCTECKNRNYSSMKNKKNTTEKIQLKKYCRHCRRHTM
                     HKETKA"
     gene            371..447
                     /locus_tag="VMU21_08870"
     tRNA            371..447
                     /locus_tag="VMU21_08870"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:405..407,aa:Trp,seq:cca)
     gene            494..679
                     /gene="secE"
                     /locus_tag="VMU21_08875"
     CDS             494..679
                     /gene="secE"
                     /locus_tag="VMU21_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HUO77674.1"
                     /translation="MVEKIKEFFREVKIEIKKVVYPSREELMGSTWVVIFTAIVISVF
                     LGIVDLGLSKIVKAALR"
     gene            684..1208
                     /gene="nusG"
                     /locus_tag="VMU21_08880"
     CDS             684..1208
                     /gene="nusG"
                     /locus_tag="VMU21_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HUO77675.1"
                     /translation="MTKNWYVVHTYSGYEEKVKLSIEDKVGKKDLKEKIARILIPTEK
                     VIELRAGKKRESDRKFYPGYILVEMDLDDETWHLVRSTPRVTGFVGGTKPVPIPEEEV
                     EIILQQLEKGPAPQVKTQFQKAENVRIIDGPFTNFVGYVDEVDMDHGRLKVMVSIFGR
                     QTPVELHFFQVEKA"
     gene            1301..1729
                     /gene="rplK"
                     /locus_tag="VMU21_08885"
     CDS             1301..1729
                     /gene="rplK"
                     /locus_tag="VMU21_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HUO77676.1"
                     /translation="MAKKEVMAQVKLQIPAGKANPAPPVGPALGPHGVNIMEFCKAFN
                     AQTQAMGDTIIPVVLTVYSDRSFTFITKTPPASELIKKAAGIVKGSGTPNKEKVGKLT
                     AAQVMEIAKTKLPDLNAYSVEEAAAIIRGTARSMGVDIVD"
     gene            1759..2451
                     /gene="rplA"
                     /locus_tag="VMU21_08890"
     CDS             1759..2451
                     /gene="rplA"
                     /locus_tag="VMU21_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HUO77677.1"
                     /translation="MGKKMDAASKKVGEGKDYSLEEAVRLVKEASYTKFDETVDMAFN
                     LGIDPKKSDQMVRGTVALPHGVGKKVRVLVFAKGEKEKEALDAGADYVGAEDLVEKIQ
                     KGWMDFDRAVATPDIMGLVGKLGKVLGPRGLMPNPKLGTVTFDVGKAVKEIKAGKVEY
                     KTEKAGIVHVPIGKVSFDAEKLVDNARVVIDAIVKAKPGTSKGKYLRKVALSSTMGPG
                     IRVDVGKSTASK"
     gene            2708..3232
                     /gene="rplJ"
                     /locus_tag="VMU21_08895"
     CDS             2708..3232
                     /gene="rplJ"
                     /locus_tag="VMU21_08895"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HUO77678.1"
                     /translation="MKKEEKGQAIAELKKKFSEAKAVILTDYKGMTVADLSVLRRLLR
                     SADVDYRIVKNTLARIASQDTPISVAKDAFKGPVAIAIGYKDPVMTAKKVIEFSRKNE
                     KLKLSGGVIDGKLYDVNEVKAIAALPPREVLLSMLAGAFQAPSSKMAAALAATVSSFA
                     YAMTALKTKKEAQG"
     gene            3304..3684
                     /gene="rplL"
                     /locus_tag="VMU21_08900"
     CDS             3304..3684
                     /gene="rplL"
                     /locus_tag="VMU21_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HUO77679.1"
                     /translation="MAVTKEQVFEFIDTMTILEMSQFVKEFEERYGVTAAAPVAVAAA
                     PGAAAAAPAVEEKTSFDVILAGAGDKKIQVIKVVRELTGLGLKEAKDLVDGAPKPVKT
                     GVTKEEAESIKAKLEEQGAKIEIK"
     gene            3761..7693
                     /gene="rpoB"
                     /locus_tag="VMU21_08905"
     CDS             3761..7693
                     /gene="rpoB"
                     /locus_tag="VMU21_08905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HUO77680.1"
                     /translation="MDRALRERHNFGKVPHFLPVPNLIEIQSRSYDKFLQKDIPMDSR
                     EEAGLQAAFKSVFPIMDYNETASLELIGYIVGEPKFSIRDCLQKGITYAAPLKIRVKL
                     NLWEAEESGNKRLKESREQEVYIGEMPLMTETGTFIVNGTERVIVSQLHRSPGVFFSH
                     DKGKTHASGRLLYSSRVIPSRGSWLDFEFDTKDILYVRIDRRKKLPATIVLKALGYSN
                     EDILKTFYPVEAVKIKGSTFTRVVSEVLAGIKAVKNIVAPDTKELIAKEGSKITKGAI
                     KKMEASGIKEIPILKEEIIDRITLKDIVDPETGEVLLEGNEAITEEIFNKILTLKVDT
                     LSLLYIDNVKYLSSLRDTLLTDKVNSQSEALIEIYKKLRPGEPPTLNAAKDLFNGLFF
                     DPKRYDLSPVGRLKINKRLDLDISLETRVLTDKDIVKIVHYLLDLRTGKGEVDDIDHL
                     GNRRIRGVGELLENQFRIGLVRMERAIKEKMTLTELETAMPHDIINAKPVMAAVKEFF
                     GSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETP
                     EGPNIGLITSLASYARVNDFGFIEVPYRKITNGRITQEVEYLSAIEGEKYIIAEATSP
                     VDKKGNLMGEAVSARVGGDFKMVTPHEVQYMDVSPKQIVGVSASLIPFLENDDANRAL
                     MGSNMQRQAVPLLQTEAPIVGTGMEYVAARDSGALVLAKRAGTVESVDASRIVVRTSE
                     DTSGVDIYSLVKFQRSNQATCINQKPIVNVGDVVQRGDVLADGSSTDMGELALGKNVL
                     VAFMPWGGYNFEDAILISERLVKDDLLTSVHIEEFEIEARETKIGPEEITRDIPNVGE
                     EALKDLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAEDVKESC
                     LYVPPGIEGVVVDVRIFSRKGTEKDERAKSIEEDDILRLQRDLEEEVRIIKEEKFRKI
                     KELIVGSKVLEDVKEAKTKEVICPAGKKLAEKHLERVKDEQLMRIKIADEDIKEKLDT
                     INNEANQYVRYLETRYDESVDRVKRGDELPPGVNKLVKVYIAMKRKLQVGDKMAGRHG
                     NKGVVAMVLPEEDMPYLPDGTPVDIILNPLGVPSRMNVGQILETHLGWAAKVLGLYAA
                     TPVFEGAKENEIKEMLKRAGIPSSGQITLHDGRTGEPFKRPVTVGYMYILKLHHLVDD
                     KIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQEFLTVKSDDVT
                     GRARMYEAIVKGDATLEPGIPESFHVLIKELQSLGLDVELLEKKSKGE"
     gene            7697..11947
                     /gene="rpoC"
                     /locus_tag="VMU21_08910"
     CDS             7697..11947
                     /gene="rpoC"
                     /locus_tag="VMU21_08910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HUO77681.1"
                     /translation="MGDDVNALFQRPKNPTDFEAIRIKLASPEKIREWSYGEVKKPET
                     INYRTFKPERDGLFCAKIFGPVKDWECLCGKYKRMKHRGVVCDKCGVEVIQSKVRRER
                     MGHIELATPVAHIWFLKGVPSRIGILLDMTMRQLEKVLYFESYIVIDPGDTPLKEKEL
                     LNEEEYKKRASEYGARFKAGMGAEAIRELLRGLALDGLAAELKAKIKDASSIGVKKKL
                     TKRLKVIEAFRQSGNKPEWMIMDAVPVLPPDLRPLVPLEGGRFATSDLNDLYRRVINR
                     NNRLKRLMELKAPSVIIKNEKRMLQEAVDALFDNGRRSRVLKTATKRPLKSLSDMIKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKIMALELFKPFVFNRLEEKGFA
                     TTLKAAKRLVEKERPEVWDALEEVIAEHPVLLNRAPTLHRLGIQAFDPVLVEGKAIKL
                     HPLVCTAFNADFDGDQMAVHVPLSIEAQVEARVLMMSVNNILSPANGKPIVVPTQDMV
                     LGLYFLTKGKGYVYAGPEEVKEAYDEGKMQEQDIITMRTETYLMETTCDKVLAGMRKK
                     SRIEGKPLVYSGPDDVRMAYDAGKLDLHKWINVRMDGQIVETTCGRVLFSEIIPSGVH
                     FKEVNKEITKKELTKIIENTYKKAGKRATVVFLDNLEKLGFDLATKSGISICMADMHI
                     PSKKPELIRVAEQEVMEIQKQYNEGLITHGERYNKVIDIWANVTERIADEMMKELGAE
                     DGKKFSEEELKERRLFNSIFMMADSGARGSTAQIRQLAGMRGLMAKPSGEIIETPITA
                     NFREGLTPLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDVIITEDDCGTSEG
                     ITVTSLVEGGEIIQPLDERIVGRFSVEDIYKPDSKELIVKKNNEITEEIAQKVMESVV
                     DRVKIRSVLTCQTKFGVCAKCYGRDLARGDLVEIGEAVGIIAAQSIGEPGTQLTMRTF
                     HIGGAAKVVVEAVLDSKNPGKAKFININAVRSREGDLVVVNRTGLIAIVDGHGREREK
                     YNLVYGAKLKVKEGDKVGAGQRLVEWDPYSTPILTEVGGRIALGDIAEGVSVKEEVDE
                     VTGLSHKVIIDYPASMRPRISIKDEHGKATLKIPGSTNLARYLLPSGAHILVEKGALV
                     SPGDILAKIPRETTKTKDITGGLPRVAELFEARRPKEQAIVTEIDGVVEFRGAHKGMR
                     VVVVKGGEEQREYHIPKGKHVSVHEGDWVKAGEPLMDGAVNPHSILDILGPNELQSYL
                     VDEVQKVYRLQGVSINDKHIETIVRQMMRKVRVEDTGDTTFLIGEQVDRTLFLEENEK
                     VKGSGGAPAQGRPLLLGITKASLTTESWVSAASFQETTRVLTEAALNAQIDELRGLKE
                     NVIMGRIVLAGSGMQKYRNTFVKRELEPFTAPEPVTEE"
     gene            12074..>12330
                     /locus_tag="VMU21_08915"
     CDS             12074..>12330
                     /locus_tag="VMU21_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HUO77682.1"
                     /translation="MNNESSHDARFIADAMLGRLSRWLRLLGFDTLYYPDIKDSDLMK
                     LAVQEGRCLLTRDTHFLNIRNFRNFLMVHSDNPIEQVAEVL"
BASE COUNT         3434 a         2581 c         3703 g         2612 t
ORIGIN      
        1 tataagtgta gtggtggagc tgataggtcc tattgcgatg gagaaggagt tgaggtttgc
       61 catcagggaa gggggaagga cggttggtgc aggcgtagtg accgaggtcc tggagtgaga
      121 aaactatgag agacataata ctttttcagt gtaccgagtg caagaacaga aattattcca
      181 gtatgaagaa caagaaaaac acgacagaga agattcagct gaaaaaatac tgcaggcatt
      241 gcagacgtca tacaatgcat aaggagacaa aggcgtagag acggtcaacg gtctgatgtt
      301 ggcagtatgc aggcagaaac tcctcatgct gtcgggtgat gaccggggct cgctgcgaaa
      361 aaatagggtt aggccagtag ctctaacggc tagagcgtcg gactccaaat ccgagggttg
      421 ggggttcaaa tccctcctgg cctgccataa taaccagttc acgagagggt gaacagcaag
      481 gggtattacg ggaatggttg aaaaaattaa ggaattcttc cgggaagtaa agatcgagat
      541 taaaaaggtt gtctatccca gcagggagga acttatgggt tccacttggg tggtgatctt
      601 caccgcgatc gtcatatcgg tttttttggg gatagtcgac ctcggtcttt caaagattgt
      661 gaaagccgcg ctaaggtaga aaaatgacga aaaattggta tgtggtacat acgtattcag
      721 gctacgagga aaaagttaag ctcagcatag aggataaggt cgggaagaag gaccttaagg
      781 aaaagattgc aagaatcctt atccctaccg agaaggtgat cgagctcagg gcaggtaaga
      841 agagagaaag cgacaggaaa ttttatcccg gatatatcct tgtggaaatg gatctggacg
      901 acgagacctg gcatttggta aggagtaccc cgagggttac cggttttgtg ggcggaacga
      961 aacccgtgcc aatccctgaa gaagaggtgg agatcatcct tcagcagctc gaaaagggac
     1021 ccgcccctca ggtgaaaacc cagttccaga aagcggaaaa tgtcaggatt atagacggac
     1081 cctttaccaa ctttgtcggc tatgtcgatg aggttgatat ggaccatgga aggttaaaag
     1141 tcatggtcag tatatttgga agacagaccc ctgtagaact tcatttcttc caggtggaga
     1201 aggcatgatt ggctcatggc tcatagcttg tggctcaaaa acggagaaca gcggttgtga
     1261 actatgaact atgaactacg aactatattt ggagggttaa atggctaaga aggaagtaat
     1321 ggctcaggtt aagctgcaga taccagcagg gaaggcaaat cccgccccac cggttggccc
     1381 tgcgttaggc ccccacggcg tgaacatcat ggagttctgc aaggcattta atgcccagac
     1441 acaggcaatg ggtgatacca taattccggt ggtattgacc gtgtattcgg acagatcctt
     1501 cacctttatt accaagacac ctcccgcatc cgagctcatt aagaaggctg caggcattgt
     1561 caagggttcg ggcacaccga acaaggagaa agtggggaag ttgaccgccg cccaggtgat
     1621 ggagatcgca aaaaccaaac tgccggatct gaacgcatac tctgttgaag aggcggccgc
     1681 aattatacgc ggcactgcac gcagcatggg ggtcgatatc gttgactaaa ggaaattagc
     1741 atatttggga ggctagagat gggaaagaaa atggatgctg ccagcaaaaa agtcggggaa
     1801 gggaaagatt attccctgga agaggcggtg aggcttgtga aggaagcctc atacacaaaa
     1861 tttgacgaga ccgttgatat ggcgtttaac cttggaatcg accccaaaaa atccgaccag
     1921 atggtaaggg gtactgtcgc gctgcctcac ggtgtgggaa aaaaggtgag ggtgcttgtg
     1981 tttgcaaagg gtgaaaagga gaaagaggcc ctggatgcgg gtgcggatta tgtgggagca
     2041 gaggacctgg tggaaaagat acagaagggc tggatggact ttgacagggc agtagcgacg
     2101 cccgatatca tgggcctcgt gggaaagctt ggcaaggtgc tgggcccaag gggattgatg
     2161 cccaacccga aactgggaac agtgaccttt gatgtgggaa aggcagtgaa ggagataaag
     2221 gcaggaaagg ttgaatacaa gacagaaaag gcggggattg tgcatgtccc catagggaag
     2281 gtttcatttg atgcggaaaa gcttgtggac aatgcgaggg ttgtcattga cgcgatagtg
     2341 aaggcaaagc ccggaacaag taaaggaaaa tatctcagga aagtcgctct ctcatcaacc
     2401 atgggtccag gaatcagggt tgatgtgggg aaatcaacgg cgagtaaata agagaggcta
     2461 aggccaaggt taagaaaata cttgatctca gcctcaacct tagcggaatg cgcggactta
     2521 gtccgttgaa atatctagtc agagacagta ggtggttact taattgtttg ttctgcccgc
     2581 ctgaggtgat caggacaaac tgcctaccga gacatccttt tgtgtggtgg tactttgagc
     2641 gactgccacg gaacagtgcg aagggtcgag aaaaggggaa aatgggtctc tgggaaagga
     2701 ggatactctg aagaaggaag agaagggaca ggccatagca gaactgaaga agaagttctc
     2761 agaggcaaag gccgtaatct tgactgatta caaaggcatg accgttgcag atctgtccgt
     2821 actgagaagg cttcttcgca gtgcagacgt ggattacagg atcgtgaaga atacccttgc
     2881 ccgtatcgca tcccaggata cgcccatttc cgttgccaag gatgcgttca aggggccggt
     2941 ggccattgcc atcgggtata aggatcccgt aatgaccgca aaaaaagtta tagagttttc
     3001 caggaagaat gagaagctta aactcagcgg cggggtgatt gacgggaagc tctatgacgt
     3061 gaacgaagtg aaggccatcg cagccctgcc gcccagggaa gtactgctgt cgatgctcgc
     3121 cggtgcgttc caggcgccat caagcaaaat ggctgctgcc cttgcggcga cggtgagcag
     3181 ttttgcctat gccatgactg cattgaaaac gaagaaagag gcacagggtt aaggttacgg
     3241 gatggacaga cccttgccct atagtgaacg aatgaacaat gaataatgaa ggaggttgag
     3301 ataatggcag taacaaagga acaggttttt gagtttattg ataccatgac gatccttgag
     3361 atgtcacagt ttgtgaagga gtttgaggaa aggtacgggg tgaccgctgc cgcgcctgtt
     3421 gcggtagcag cagcgcctgg agcggcagct gcggctccgg cggtcgagga gaaaacaagc
     3481 tttgacgtga tactcgccgg cgcaggtgac aagaagatac aggtgatcaa ggtggtgaga
     3541 gagcttaccg ggcttggcct caaagaggca aaagaccttg ttgacggcgc tccgaaaccg
     3601 gtgaagacag gggtcacgaa agaagaggct gagtctatca aggcgaaact cgaagagcag
     3661 ggcgcaaaga tcgagataaa gtaacaacag atgagggtga aggcggagga tttttcttcg
     3721 ccttcaccct catctgaacc ttattcacag aaggagatat atggacaggg ctttgagaga
     3781 gagacataat tttggtaagg ttccccattt cctgcctgtg ccgaacctta tcgagataca
     3841 gagccgctcg tacgataaat tcctccagaa ggacattccc atggacagta gggaggaagc
     3901 agggctccag gcagccttta agagcgtgtt tcccatcatg gactataatg agactgcgtc
     3961 tttggagctc attggataca tcgtcggcga gccaaagttc agtattagag attgcctgca
     4021 aaaggggatc acctatgctg ccccacttaa gataagggtt aaacttaacc tgtgggaagc
     4081 ggaggagagt ggcaataaga ggcttaagga gtccagggag caggaggtgt acatcgggga
     4141 gatgcccctg atgacagaga cagggacgtt tattgtgaac ggcaccgaaa gagttatagt
     4201 gagccaactt caccgttccc ccggtgtttt cttcagccac gataagggga agacccatgc
     4261 cagtgggaga ctcctgtatt cttcaagggt gattccgtca cgggggtcgt ggcttgactt
     4321 tgaattcgac acaaaagaca tcctttatgt gcgcattgac aggcggaaaa agcttcccgc
     4381 aaccattgtg ctcaaggcgc tgggatactc taatgaggat attcttaaaa ccttctatcc
     4441 tgtggaggcg gttaagatta aaggatcgac ctttacaagg gtggtgagcg aggtgttggc
     4501 ggggataaag gcggtaaaga atatcgtggc gcctgacacg aaggaactca tcgcaaaaga
     4561 gggaagcaag atcaccaagg gtgccataaa gaagatggaa gcatccggta tcaaagagat
     4621 ccctatactg aaagaggaga ttatcgacag aatcacactt aaggatatcg tggatcctga
     4681 aacaggcgag gtgctgctgg agggcaacga agccatcaca gaagagatct ttaataagat
     4741 tctcaccctc aaggtggata ccctgagcct tttgtacata gacaatgtca aatatctctc
     4801 ttccttgcgc gacacgctct taacggataa ggtcaatagc cagtctgagg cgcttatcga
     4861 aatatacaag aaactgaggc cgggagagcc gccgaccctt aatgctgcaa aggacctgtt
     4921 taacggactt ttcttcgatc caaagagata tgacctttct cctgtgggga gattaaagat
     4981 caacaagagg cttgatcttg acatttccct cgagaccagg gttcttaccg ataaggatat
     5041 cgttaagatt gtgcattatc ttctcgatct caggacggga aagggagagg tggacgatat
     5101 tgaccatctg ggaaacagac ggatacgggg tgtgggagaa ctgttggaaa accagttcag
     5161 gatcggactt gtgagaatgg agcgtgccat caaggagaag atgaccctca ccgaactgga
     5221 gaccgccatg ccccatgaca ttattaatgc aaagccggtc atggctgcgg tcaaggagtt
     5281 cttcgggtcc agtcagctga gtcagtttat ggatcagacc aatccccttt ctgagattac
     5341 ccacaagagg aggctctctg cccttggtcc gggtggtctt acgagggaac gggcggggtt
     5401 tgaggtgagg gatgtgcatc ctacccacta tggaaggata tgtcccatag agacccctga
     5461 aggtcccaat ataggactga tcacctccct tgcctcctat gcgagggtga atgattttgg
     5521 ttttatcgag gtcccttaca ggaagattac caacgggagg attactcagg aggtcgagta
     5581 tctctccgcc attgagggcg aaaaatatat catcgccgag gctacgtctc ccgttgacaa
     5641 gaaaggtaac ctcatgggcg aagcggtttc tgcgagagtg ggcggggatt tcaagatggt
     5701 gacaccccat gaagtccagt atatggatgt ttcgccgaag cagattgtgg gagtttcggc
     5761 ttcgctgatt ccatttctcg agaatgacga tgcaaacagg gctctcatgg gttctaacat
     5821 gcagagacag gcggtgccgt tgctccagac cgaggcaccc attgtgggga ccggtatgga
     5881 atacgtggca gccagggatt ccggtgccct tgtgcttgca aaaagggcgg gtactgtaga
     5941 aagtgtagat gcatcacgga tcgtggtgag gacgtccgag gataccagcg gtgtggatat
     6001 ttacagtctg gtaaagttcc aacgatcaaa ccaggcaacc tgtatcaacc agaagcccat
     6061 cgtgaatgta ggtgacgtgg tacaaagggg agacgttctt gctgatggtt catcaactga
     6121 tatgggagaa ttggccctgg gcaagaatgt gcttgtggcc ttcatgccat ggggaggata
     6181 taactttgag gatgcgatcc tcatcagtga gagacttgtc aaggatgacc tcctcacctc
     6241 agtgcacata gaagagttcg agatcgaggc gagggaaaca aagatcgggc ctgaagagat
     6301 cacgagggat attcccaatg tgggagaaga agccctcaag gaccttgacg agagcggcat
     6361 tatacgaatc ggtgccgagg tgaagccagg ggatatcctt gtgggaaagg ttacccccaa
     6421 gggcgagacc cagcttaccc ctgaagagaa actgctcagg gcaatttttg gtgagaaggc
     6481 agaggatgtg aaggaaagct gcctctatgt cccgcccggc attgaggggg ttgtggttga
     6541 cgtgaggata ttttcgagga agggtaccga aaaagacgaa cgggcgaaga gtatcgagga
     6601 agatgatatc ctcaggctcc agagggacct tgaggaagag gtaaggatca ttaaagagga
     6661 gaagttcagg aagataaagg aattaattgt gggcagcaag gttctggagg acgtgaagga
     6721 ggctaagacg aaagaggtta tctgtccggc aggaaagaag ctcgctgaga agcatcttga
     6781 gagggtgaag gatgaacagc tgatgcggat aaagatagct gacgaggata ttaaggagaa
     6841 gctggacact attaacaacg aggccaacca atatgtccgt tacctggaaa cgcgctatga
     6901 tgagagcgtt gaccgtgtca aaagaggtga cgagctgccc cctggggtga acaagcttgt
     6961 gaaggtgtat atcgcgatga aacgtaagct ccaggtggga gacaaaatgg caggaaggca
     7021 tgggaataag ggggttgttg ccatggtcct ccctgaagag gacatgccgt atctccctga
     7081 cgggacgccg gtggatatca ttctgaatcc cttgggcgtt ccttcccgga tgaatgttgg
     7141 gcagattttg gaaacccatc tcggctgggc tgcaaaagtg ttgggcctgt atgcagctac
     7201 cccggttttt gagggtgcaa aggaaaatga gataaaggag atgttgaaaa gagcgggtat
     7261 cccctcatcc ggccagataa cccttcatga cggaaggaca ggagaaccgt ttaaaaggcc
     7321 tgtcacggtg ggatatatgt atatactaaa actccaccac ctcgtagacg acaagattca
     7381 tgcgcgttcc ataggtcctt actcccttgt tactcagcag cctctgggag gcaaggcaca
     7441 attcggcggt cagagactgg gcgagatgga ggtgtgggcc cttgaggcat atggtgcagc
     7501 ctacaccctt caggaattcc tgaccgtcaa gagtgatgac gttaccggaa gggcgcggat
     7561 gtatgaggct attgtgaaag gtgatgcgac tctggaacca gggattccgg aatccttcca
     7621 tgtattaatt aaagaactcc agagccttgg ccttgacgta gagcttctgg agaaaaaaag
     7681 caagggggaa taagtcttgg gagacgatgt gaatgccctt tttcagagac caaagaatcc
     7741 gacagatttt gaggcgataa ggatcaaact cgcctcgcct gagaagatca gggaatggtc
     7801 ctacggagag gtgaagaagc cggagaccat taattatcgt accttcaaac ctgagaggga
     7861 cggactattc tgcgcaaaga ttttcggacc tgtgaaggac tgggaatgcc tgtgtggaaa
     7921 gtacaagagg atgaagcaca ggggtgttgt ctgcgataag tgcggtgttg aagtcatcca
     7981 gtcgaaggtg agaagggagc gcatgggcca catagagctt gcgacccctg tggcgcatat
     8041 ctggtttctt aagggagtac cgagcaggat tggaatattg ctcgatatga ccatgagaca
     8101 actggagaaa gtgctctatt tcgaaagcta cattgtcatc gaccccggag acacccctct
     8161 gaaggaaaaa gaactgctta acgaagaaga atacaagaag agggcgtctg agtatggcgc
     8221 acgtttcaag gcgggaatgg gtgcggaggc gatacgtgag ctcctgagag ggcttgcact
     8281 ggatggcctc gcagctgaac tcaaggcaaa aataaaggat gcgtcatcca tcggcgtcaa
     8341 gaaaaaactc acgaagcgac tgaaggtgat tgaggcattc agacagtcag ggaataaacc
     8401 tgagtggatg attatggatg ctgtcccggt actcccccct gacctgagac ccctggttcc
     8461 tctggaggga ggacggtttg ctacgtccga ccttaatgat ctctatcgga gggtaatcaa
     8521 caggaataac agactgaagc ggttgatgga actcaaggcc cccagtgtta tcatcaagaa
     8581 tgagaaaagg atgctccagg aggcggttga cgccctcttt gataacggca gaaggagcag
     8641 ggttcttaag accgcgacga agaggcccct caagtccctc agtgacatga tcaagggaaa
     8701 acaggggagg ttcaggcaga atcttctcgg caagagggtt gactattccg gtcgttcggt
     8761 aatcgtggtg ggacctgaac tgaaactcca ccagtgcggt ctcccgaaga taatggcgtt
     8821 ggagctcttc aagccttttg tttttaacag actcgaagaa aaggggtttg caactacact
     8881 caaagctgcg aagaggctgg tggaaaagga gcgtccggag gtatgggatg ccctggaaga
     8941 ggtgatcgcg gagcatccgg tgctcctgaa ccgtgcccct acccttcacc ggcttggcat
     9001 ccaggcgttt gatcccgtcc ttgtagaggg caaggcaatc aagctccatc ccttggtctg
     9061 cacggccttt aacgcggact ttgacggcga ccagatggcg gtccatgtgc ccctttctat
     9121 agaggcgcag gtagaggcga gggttttgat gatgtcagtg aacaacatcc tctccccagc
     9181 aaacgggaaa cccattgttg tgccgacaca ggatatggta ctgggcctct acttcctcac
     9241 aaagggtaag gggtatgtgt atgcaggacc tgaagaggtt aaggaagcct acgatgaggg
     9301 taagatgcag gaacaggata ttatcacgat gaggaccgaa acctatctta tggaaactac
     9361 gtgtgataag gtccttgcgg gcatgaggaa gaaatcgaga attgaaggga agcctctcgt
     9421 ctattcgggt cccgatgacg tgagaatggc ctatgatgcg ggtaagctgg atctgcataa
     9481 atggatcaac gtgcggatgg atggacagat tgttgaaacg acctgtggca gggtactgtt
     9541 cagcgagatc attccctcgg gggtacattt taaagaggtg aacaaggaga tcacgaagaa
     9601 agaacttacc aaaataatag agaataccta taagaaggcc ggaaagcggg ccacggtggt
     9661 attccttgat aaccttgaga agctggggtt cgaccttgcc acaaagtcag gtatatccat
     9721 ttgcatggct gacatgcata tcccctccaa gaaacctgag ttgatacggg tcgcggaaca
     9781 agaggttatg gagatacaga aacaatacaa cgagggactc atcacgcacg gagagcggta
     9841 taacaaggtg atcgatatat gggcgaatgt gaccgagagg atcgctgatg agatgatgaa
     9901 ggaacttggg gccgaggacg ggaagaagtt ctcggaggaa gagctcaagg agagaaggct
     9961 ttttaacagt atctttatga tggccgactc cggcgcaagg ggaagcactg cccagatcag
    10021 gcagcttgcg ggtatgaggg gacttatggc aaaaccctcg ggcgagatta tcgaaacgcc
    10081 cattaccgcg aacttcaggg aaggtcttac tcccttgcag tacttcatct ctacccatgg
    10141 tgcgaggaag ggacttgcgg acactgccct caagacagca aattcagggt atctcaccag
    10201 aaggcttgtg gatgttgcac aggatgtcat catcaccgag gacgactgcg gcacctcaga
    10261 ggggatcacc gtcacctccc ttgtggaagg aggagagatc atccagccgc tggatgagag
    10321 gatcgtcggc agattctccg tagaagatat ctataagcct gattcaaagg agctaatcgt
    10381 aaagaaaaat aatgagatta ccgaggagat tgcccaaaag gtgatggaat cggtggttga
    10441 cagggtgaag atacggtctg tgctcacatg ccagacgaag ttcggtgtgt gtgccaagtg
    10501 ctacggaagg gatctggcga ggggcgatct tgtggagatc ggtgaggcag tgggaatcat
    10561 tgcggcgcag tccattggtg aaccgggcac ccagctgacc atgagaacct tccatatcgg
    10621 tggtgccgca aaggtagtag tagaggcggt ccttgattcc aagaatccgg ggaaagcgaa
    10681 gttcataaat atcaacgcag tgagaagcag ggaaggtgac cttgttgtag tcaataggac
    10741 cggcctcatt gctattgttg acggacacgg aagagagagg gagaaataca atctcgtata
    10801 tggagcaaaa ctgaaggtga aggaaggcga caaggtcggg gcgggccaga ggctcgtgga
    10861 atgggacccc tactcaacgc ccatactcac cgaggtggga ggaagaatag ccctcggcga
    10921 tattgcggag ggggtgtcgg tgaaggagga ggtcgacgag gttactggac tttctcacaa
    10981 ggtcatcatc gattaccccg cgtccatgag gccgaggatc tccataaaag atgagcatgg
    11041 gaaagcgacc ctgaagatac cgggatctac gaatctcgcg cgatacttat tgccatcggg
    11101 cgcccatatc cttgtggaaa agggagccct tgtctctccg ggcgacattc ttgcaaagat
    11161 acccagagag accacaaaga caaaggatat cacgggtggc cttccaaggg ttgctgagct
    11221 ttttgaagcg agacgaccaa aggaacaggc cattgtcaca gagatcgacg gtgtcgtaga
    11281 attccgtgga gcacataagg gaatgcgggt cgtggtggtg aagggtggag aggagcagcg
    11341 tgagtatcat atccccaagg gcaagcatgt gagcgtccac gaaggtgatt gggtaaaggc
    11401 aggggagcct ttaatggatg gggcggtgaa tccccacagc atcctcgata tcctcggacc
    11461 gaatgaactc cagagttatc tcgtggatga ggtgcagaag gtataccggc tccagggtgt
    11521 ttcgatcaat gacaagcaca tcgaaaccat tgtgcgccag atgatgagaa aggtgagggt
    11581 tgaagatacg ggcgatacga cgttccttat aggagagcaa gttgaccgga cactcttcct
    11641 ggaggaaaat gagaaggtga agggatcggg aggggcgcca gcgcagggaa ggccccttct
    11701 cctcggcatc accaaggctt cccttactac agaaagctgg gtttctgctg catcttttca
    11761 ggagaccaca cgggtactta ccgaggctgc gctgaatgcc cagatagatg aattgagagg
    11821 attgaaggaa aatgtcatca tgggcagaat tgttcttgcc ggttcaggga tgcagaagta
    11881 ccgcaacacc tttgtgaaga gggaactgga gccattcact gcccctgagc ccgtaacgga
    11941 agagtgacgc aaaggcgtgg ggtgctcgtt cctcactgag agcgcggaaa aacagatcgg
    12001 gcgtctcccc tgacatcaag agactgatgt cgagaagacg cccggtattt tattgggcga
    12061 tgagctgttg agcatgaaca atgaatcctc ccatgatgcg cgtttcatcg cggatgccat
    12121 gcttggccgt ctctcccggt ggctgcgact tctcggtttt gataccctct actatccgga
    12181 tataaaggac agcgatctca tgaaattggc ggtgcaggag ggcagatgcc ttctcaccag
    12241 ggatacacac ttcctgaata tccggaattt ccggaacttc cttatggtac actcggataa
    12301 tcccatagaa caggttgcgg aggtgctcag
//
LOCUS       DATKOU010000192         5678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4119629, whole genome shotgun sequence.
ACCESSION   DATKOU010000192 DATKOU010000000
VERSION     DATKOU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119629"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VMU21_08920"
     CDS             <1..758
                     /locus_tag="VMU21_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO77683.1"
                     /translation="IVDGTDSNTAMVALSYATKVIAKYASEQGANTVGAVLFPLDFRT
                     RVWYNPDLKSRNYNVSGVIASIIMITCIMLTSMAIVREREVGTMEQLMVTPIRPVELM
                     LGKTIPFAIIGLLDMFIVTLAGVLWFGITIKGSLLLVLMGTSVYLLSILGIGLFTSTI
                     LKTQQQSLLATMFFNMPAMLLSGFMFPIENMPVIFQYITYLDPLRYFLVIIRGIFLKG
                     NGFDILWPQMAALFVLGTVIITISSFRFRKRLG"
     gene            956..1216
                     /locus_tag="VMU21_08925"
     CDS             956..1216
                     /locus_tag="VMU21_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77684.1"
                     /translation="MGYTNVALKDKIEAMYPEIEKHGITVGLDFNKEKNTYIVTFRKG
                     VHELTTHLEKKDADECMDGIKCVHLGIQIGQFVKNFEIIEKE"
     gene            complement(1228..2154)
                     /locus_tag="VMU21_08930"
     CDS             complement(1228..2154)
                     /locus_tag="VMU21_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HUO77685.1"
                     /translation="MGKWLLAISLFIVAVLALPPISSATTEYARQTRMQCKDCHVEAI
                     GSGTLTNTGQQFLADMKLKGLYRPLSTIQRIVRFWIGYIHLLTAIVWFGTIFYVHILL
                     KPAYAAKGLPKGELFLGWISIIIMSVTGILLTVARIPAWKVLFTTRFGILLSIKIVLF
                     LIMAATAFVVTFIIGPKLRRKLKTRWETDISLGKQDLTAEELHSFDGKEGRPAFVAYK
                     GKIYDVTKSRLWKDGSHARKHHAGRDLTDALKTAPHAEDKLLSMPEVGKLIGAGQKIP
                     KPLPERVFYVLAYINLGFVFLITFIIALWRWW"
     gene            complement(2236..3627)
                     /locus_tag="VMU21_08935"
     CDS             complement(2236..3627)
                     /locus_tag="VMU21_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HUO77686.1"
                     /translation="MTVKYEGQKIAQNFYTFAVLLFLVQVLVGIIAAIQFMKPDFFIL
                     NFNIIRTLHINSLVVWLLVGFMGATYYVITEESETELWNIPLAKFQFWATVLTITLVV
                     LGYIWMGINPQANSLIMGINPLNEGREYIEAPRWADILIVVSVLLFLFNCVMTVLKTK
                     KWTGIQGVLLSGLVFLALMYIPGMFYTKSMVKDQFWWWWVVHLWVEGSWEIIAGALLA
                     FMLMKTIGAKREVLEKWMYIEVGLVLFTGILGTGHHYYWIGTPSYWLWVGGIFSSLEP
                     VPLLFMVWDAFRTTREARNVTNRPGLFYTVAHAIFNFVGAGLWGVIHTLPQVNKWTHG
                     TQLTTAHGHLAFYGAYVLLVIAMIYVTLPSIRGVKEYNPSRAYLSFWWMTISMVFIVL
                     TITGAGMVQTYMERLMGLDYVAVKTTYNFWFWVLRAIFGVGFLIGVGIFVIDYFKLGK
                     EPMPVISAAPEKA"
     gene            complement(3640..4314)
                     /locus_tag="VMU21_08940"
     CDS             complement(3640..4314)
                     /locus_tag="VMU21_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUO77687.1"
                     /translation="MSNKAAKNLFIFGSLFFFVIFLALTFDTMSTLDKRAPEITEKVN
                     AGKMVWHKYDCIGCHTILGNGSYFAPDMTKVVERKPAGYLKKFLMDPKAVNPKAAMPK
                     FGITSEEADNLLTFLDWISKVDTNGWPPKPILAVAAGVGGGELTSGQKIYQAQGCSGC
                     HMINGIGGTTGPELTKVGSRRDGKWLHEHFEDPSKVVANSAMPSYKHLGDQALNELTE
                     YMLTLK"
     gene            4433..4984
                     /locus_tag="VMU21_08945"
     CDS             4433..4984
                     /locus_tag="VMU21_08945"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUO77688.1"
                     /translation="MKKYKIVDKKTEWEGKFLRGVRISYRDSAGILRKWESVERRNCD
                     GIVAVVPFTDEGEVIFIRQFRPPVNNYVIEFPAGLNDRGESTENAARRELLEETGYEA
                     KEMVFLAKGPLSSGSSGEILTAYLARGLRFKGIGQRDETEDIEVLKVPGDQVYERLSR
                     LEAEGNFIDLKVFGLLEMAKRLL"
     gene            5134..5457
                     /locus_tag="VMU21_08950"
     CDS             5134..5457
                     /locus_tag="VMU21_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77689.1"
                     /translation="MNKQDIKRNCWEFKNCGRQTGGEKAKELGVCPATTDEKLDGVHG
                     GRNAGRACWVLAGTMCGGKVQGTFAQKYENCELCDFFRSVKEEEGPAYQLSIVLLNKV
                     RAFEK"
BASE COUNT         1490 a         1322 c         1479 g         1387 t
ORIGIN      
        1 tgattgttga cggtactgat tccaataccg ccatggtcgc cctgagctat gcaacaaagg
       61 taatcgcaaa atatgcgtcg gaacagggag caaatactgt aggcgcagtg ctctttcctc
      121 tggacttcag aacaagggta tggtataacc ctgatcttaa atccagaaac tacaatgttt
      181 ccggagtcat cgcctccatc atcatgatta cctgcatcat gctcacctct atggcaattg
      241 tgcgggaaag agaggtgggc accatggagc agctcatggt gacgcccatc cggcccgtgg
      301 agctcatgct ggggaaaacg atcccctttg ccattatcgg cctcctggat atgttcatcg
      361 tgacactcgc gggggtactc tggttcggga tcaccataaa gggaagcctt ctgcttgtcc
      421 tcatgggcac ctcggtgtat cttctttcga tactcggcat agggcttttc acatccacca
      481 tactcaagac gcagcagcag tcgttgctcg ccacaatgtt ctttaacatg cccgccatgc
      541 tcctttccgg ctttatgttt cccattgaga acatgccggt aatctttcaa tacataacct
      601 atctcgatcc cctcaggtat ttcctcgtga tcatacgggg gattttcctc aaagggaatg
      661 gtttcgatat tctctggccc cagatggctg cgcttttcgt gctgggtacg gtgatcatca
      721 ccataagctc ctttcggttc cgtaagaggc ttggataagg ggaatttcct gtctttgccc
      781 tgtccccaat gcagaacaca agaaagggat ggacaagtcc aagatatcaa ttagaatagg
      841 gatataagaa caataaatcg taaccgttaa aggtgaacgc ttcaggggag ggcagcttct
      901 ttagggtgta gggagaccgc gcaaataaaa ccatttttct gtaaaaggag ggaacatggg
      961 ttataccaat gttgcactga aggataagat agaagcgatg tatcctgaaa tcgaaaaaca
     1021 cggcatcacg gttggtcttg acttcaacaa ggaaaagaat acttacatcg taacgttcag
     1081 aaagggcgtg catgaactca ccacccacct tgagaaaaag gacgctgatg aatgcatgga
     1141 cggcatcaaa tgcgtgcatc tcggaattca gataggacag tttgttaaga atttcgagat
     1201 catcgagaag gagtagcgag acacacgcta ccaccatctc cagagggcga tgatgaaggt
     1261 gatgagaaag acaaagccga gatttatgta cgcaaggaca tagaacaccc tttcgggcag
     1321 gggttttggt atcttctgcc ctgccccgat cagttttccg acctcgggca tggagagtaa
     1381 tttgtcttca gcatggggcg cggtcttgag ggcatcggta aggtcacgtc cggcatggtg
     1441 ttttctggca tgagaaccat ccttccagag cctggacttt gtcacgtcat atatcttccc
     1501 tttataggcg acaaacgcag gtcttccctc cttgccgtca aaactatgca gctcttcagc
     1561 agtcaggtcc tgctttccca ggctgatatc ggtctcccac cgggtcttta acttccgccg
     1621 cagcttgggt cctataataa atgtcaccac aaaggctgtg gctgccatga tgagaaagag
     1681 aactatttta atgctcagga gtatgccgaa gcgtgtggtg aagaggacct tccatgcagg
     1741 gatcctcgca acggtgagga ggatgcctgt aacagacatg atgatgatgg agatccagcc
     1801 gaggaagagc tctcccttgg ggagtccctt tgcagcatag gcaggtttca gaaggatatg
     1861 tacatagaaa atcgtcccga accagacaat ggcggtaagc aggtggatat agcctatcca
     1921 gaaccgcacg attctttgta tggttgaaag gggtctgtaa aggcccttaa gcttcatgtc
     1981 cgcaagaaac tgctgccctg tatttgtgag ggtgccgctg cctatggcct caacatgaca
     2041 gtccttgcac tgcattcttg tctgtctggc gtattctgtc gttgcggaag atatgggagg
     2101 aagggccaac accgccacaa tgaagagaga gatcgctaaa agccatttgc ccatgggaac
     2161 attatacccg gtttgttcta gaaaattaag ggaaggggat ttgaaaaggg gggacagtgt
     2221 cccccctttc gcgtgttatg ccttttcagg ggctgcgctt atgaccggca ttggctcttt
     2281 accaagcttg aagtagtcga tgacaaagat tcctacccca atgaggaagc cgacaccgaa
     2341 gatcgccctc aatacccaga accagaaatt gtaggtggtc ttgaccgcaa catagtcaag
     2401 ccccatgagc ctctccatgt aggtctgtac cattcccgca cccgttatgg tcaggacgat
     2461 gaagaccatg gagatggtca tccaccagaa tgataggtac gcccttgatg gattatactc
     2521 cttgacgccc cttatcgagg gcagcgtaac gtatatcatc gcgatcacaa gcagtacata
     2581 ggcgccatag aatgcgaggt ggccgtgcgc agtggtgagc tgcgtgccgt gagtccattt
     2641 attgacctgg ggcaatgtgt gtattactcc ccataaccct gcgcccacaa aattgaatat
     2701 ggcatgggca acagtgtaga aaagcccggg tctgttcgtg acgtttcgtg cttctcttgt
     2761 tgtcctgaac gcgtcccata ccatgaagag aaggggaacg ggctcaagtg agctgaaaat
     2821 gcctcccacc cataaccagt aagagggggt tccgatccag taatagtgat ggccagtacc
     2881 gaggatgccg gtgaagagga caaggcccac ctcgatgtac atccacttct cgaggacctc
     2941 cctctttgcg ccgatggttt tcatgagcat gaaagcgagg agcgcgcccg cgatgatctc
     3001 ccatgagcct tcgacccaga ggtgaaccac ccaccaccac cagaactggt ctttcaccat
     3061 actctttgta taaaacattc ccggaatgta catgagggca aggaacacaa ggccgctgag
     3121 gagcacgccc tgtatgccgg tccatttctt tgtcttcagc actgtcatga cgcagttaaa
     3181 aaggaaaagg agtaccgaca ccacgatgag gatatctgcc cagcgtggcg cctcgatgta
     3241 ctccctcccc tcattgaggg ggtttattcc catgatgagg ctgtttgcct gggggttgat
     3301 gcccatccat atataaccga ggaccacaag tgtgatcgtg agcactgttg cccagaactg
     3361 gaactttgcc agggggatat tccataattc cgtctcagat tcctcggtaa tgacataata
     3421 tgtggcaccc atgaagccca cgagcagcca caccaccaag gagtttatat ggagagtcct
     3481 tatgatgtta aagttcagaa taaagaaatc gggtttcata aactggatgg ccgcgatgat
     3541 gccgaccaaa acctgtacca gaaagagaag cacagcaaag gtgtaaaaat tctgtgctat
     3601 cttttgacct tcgtatttta cagtcatctt ccgtacctcc tatttaagag tgagcatata
     3661 ttcagttaat tcattcaatg cctggtcgcc aaggtgcttg tagctcggca tggctgaatt
     3721 ggccaccacc tttgaggggt cctcaaagtg ttcgtggagc cacttcccgt ccctcctgct
     3781 tcccaccttt gtgagctcag gtcctgtagt accgcctatg ccgttgatca tgtggcagcc
     3841 agagcagccc tgggcttgat atattttctg tcctgaggta agttctcctc cgccgacgcc
     3901 tgctgctact gcaaggatcg gttttggagg ccatccattg gtgtccacct tcgagatcca
     3961 gtcgaggaat gtgagaaggt tgtctgcctc ttctgaagta atgccgaatt taggcatggc
     4021 tgcctttgga tttactgctt tggggtccat gaggaatttc ttaagatatc ctgcgggttt
     4081 cctttccacc actttggtca tgtcgggtgc aaaatatgac ccgttaccca gaatggtatg
     4141 gcacccgatg cagtcatatt tgtgccagac cattttcccg gcattcacct tttccgttat
     4201 ctctggcgcc cttttgtcca gtgtgctcat ggtgtcaaag gtgagggcaa gaaagataac
     4261 aaagaaaaag aggctgccga agatgaagag attctttgct gccttgttac tcatatacac
     4321 ctcccaatag ttcagcggct tgttgcctat agtcattatt gaaaaaaaag catgacttat
     4381 ctatgattta ggtcataacc ctgtaatatt tagaaaggcc tgtgaaaaat aaatgaaaaa
     4441 atataagatt gtggataaaa agactgaatg ggaagggaaa tttctcaggg gagtcaggat
     4501 aagctacagg gattccgcgg gtattctgag gaaatgggag tcggtggaga gaagaaactg
     4561 tgacggaatc gtagccgtcg ttcccttcac tgatgaggga gaagtgatct ttatcaggca
     4621 gttcaggccc ccggtgaaca actatgtcat cgaatttccg gcaggactga atgacagggg
     4681 ggagagcacc gaaaatgctg cccggcggga gcttcttgaa gagacggggt atgaagcgaa
     4741 ggagatggtg ttcctcgcaa agggaccact ttccagcggc tcctcagggg agattctgac
     4801 cgcctatctc gccaggggat tgagattcaa agggatagga caaagggacg agaccgaaga
     4861 catagaggtc ctgaaagtgc cgggtgatca agtgtacgag cggctgtccc gtctggaggc
     4921 ggaagggaat ttcatcgacc tcaaggtgtt cggactcctg gagatggcaa agaggctgtt
     4981 gtaggtatct aaatcccttc ccgcgttcca agtttcttca tcatattccg cagacctgca
     5041 aaaaatgcat aaaaaaaagc aagaaatgca tagaaatgtt catttggttt tgacatcctt
     5101 tagggagtaa gcctttccta agggaggaga acgatgaata agcaagatat aaagcgaaac
     5161 tgctgggagt tcaaaaactg tgggagacaa actggcggcg agaaggccaa agagcttggc
     5221 gtatgccctg cgacaacaga cgaaaagctg gatggtgttc atggcggaag aaacgcggga
     5281 cgggcatgtt gggtgctggc aggcacgatg tgcggtggaa aggtccaggg cacatttgcc
     5341 cagaagtatg aaaactgtga gctctgtgat ttttttcggt cagtgaaaga agaagaaggt
     5401 ccggcatacc agttgtcgat agtccttctc aataaagtca gggcgtttga aaaataatac
     5461 aggttttgct gtgccttctt tctgtacatc gcacttacgg aactaggccg aggaatgcag
     5521 ggtactatcg aaaacaaata aaacaaatag ggacacaggg catgtcccag gcttgccata
     5581 actatgaaga ttaccatcgt agtcaagaaa atgatcagaa agttctcggg caccgggttc
     5641 tgtgagaaac agacggcaga cggtttttat tgaatctg
//
LOCUS       DATKOU010000193         7589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4888069, whole genome shotgun sequence.
ACCESSION   DATKOU010000193 DATKOU010000000
VERSION     DATKOU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4888069"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VMU21_08955"
     CDS             <1..739
                     /locus_tag="VMU21_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO77690.1"
                     /translation="GRLNREGVMRLAEALQRHLTEPLLDAVAEESDTFTRSFLLSVLV
                     GFKGTILPHAARRINDKRWYVVRNVLYLLRECGGANYMHIVRPMTKHADRRVSMEALK
                     TLLSFNAPGAFSYLRICLESSEPEVRDQAVKLAGTTRVKEAVPYLLQLLEKRDLVGIE
                     LEQKASIIRALGDIGDPRAVDTLERLYRAKSLLFRNAMEELRLEIVRSLRNYPSESVT
                     PLLERGAKSENNEIRKISEEILNREKD"
     gene            741..1832
                     /locus_tag="VMU21_08960"
     CDS             741..1832
                     /locus_tag="VMU21_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUO77691.1"
                     /translation="MSQKKANGFISLIMTALSNCALYSEDHPLVRDFSVKAHGEIGGL
                     YVNDSLALTLLGDSLVFNETPLTDAGPHLHSFTKKLKRKGIEKIVIEKGVTSDEIKKF
                     VAAIASTEKVISSPHISVGMVEVKFGAEGDDVSTIVRENIAKVKALFQDVNKFRRLDM
                     VSLEDAVIAFIATLKREMNVLRVISPVKSYSEYTYVHATNVSVITLFQAEALGLKGEN
                     LHDVGIAALMHDVGKMFVAKEVLEKPGKLDDKEWDQMKQHPAAGAKYLSALPDVPKLS
                     MITAFEHHMKFDGSGYPSTKRQSKKQHIVSQMVAIADFFDALRTERPYRKAMDIATII
                     GLIKEATGKDFNPLLVENFFSALKQIKAA"
     gene            complement(1819..2814)
                     /locus_tag="VMU21_08965"
     CDS             complement(1819..2814)
                     /locus_tag="VMU21_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO77692.1"
                     /translation="MDNSSAMEHDISCSRSSSGYAHHLGTYRRIAQITFILFIFLMPV
                     LNIFRYDSSTKELIVLGQVWSLGLKQGFYADHSISGATHVALHFFLKAILPWVLLLSV
                     FPLLGFLTGRSFCGWLCPEGALFEWADFLTVKLLGRRSLYGKRENDPDKGKPNKPFWL
                     LVAVLSIVLIPLCGGIALTGYFVAPKTVWYQVTHGDFTFGVKAGIIGVAVYMLIGSIF
                     VRHTFCKYVCAAGLMQMLFGWVSPVSLRLKLDTARIGECTDCKKCEKACFMNVLPRKN
                     KRDVSCVNCGACIDACNRELAGRGLFHYSTGDATCLAPAENCALATQALSRAKQL"
     gene            3126..3626
                     /locus_tag="VMU21_08970"
     CDS             3126..3626
                     /locus_tag="VMU21_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HUO77693.1"
                     /translation="MRKTLLVLGILLVVGLGVFLLSYLREPREEGVLALSGNVEVTEY
                     DMGFKFSGRVEKLFTDESLKVTKGDPLAALDSAELESQVSQARAYVKEAEVRLKELKA
                     GSRPQEIEQARANLRFVEAELEKVRKDFERAERLFRQDARFPSLTLQRACTTPVSHSK
                     RVHRNS"
     gene            3722..4054
                     /locus_tag="VMU21_08975"
     CDS             3722..4054
                     /locus_tag="VMU21_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUO77694.1"
                     /translation="MKDTVIYAPISGVVIRKNIELGETVGTGIPVFTLGDLENPWMKV
                     YVKEDKLGLVKLGQKAKVTVDSYPGKVYEGTVTFISSEAEFTPKNVQTKEERVKLVFG
                     VKVRVKRQ"
     gene            4268..5188
                     /locus_tag="VMU21_08980"
     CDS             4268..5188
                     /locus_tag="VMU21_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO77695.1"
                     /translation="MGNVAVRASKLTKLFGTTPAVDALGLEIRKGELFGLVGPDGGGK
                     TTILRLLTAILQPSSGEAWVAGYSVIHEGERIKEKIGYMSQKFGLYEDLTVMENIMFY
                     ADLYAVPAGERPKRIERLLGFSNLTPFTGRLAGKLSGGMKQKLGLACALIHTPEVLFL
                     DEPTNGVDPVSRRDFWRILYDLLKEGVTVVISTSYLDEAERCTRIGLIHKGKILMEDI
                     PTEIRKSLRTNMVEVWTDDSRKAADLIRKGEGIRGVSVYGDRLHIALVNKDLLDSIFQ
                     TLQEEGIIVRDHREILPSIEDVFISMVEKQ"
     gene            5185..6111
                     /locus_tag="VMU21_08985"
     CDS             5185..6111
                     /locus_tag="VMU21_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO77696.1"
                     /translation="MNQIDSAVKVEGLTRRFDDFVAVNGINLDIGKGEIFGFLGPNGA
                     GKSTTIKMLCGLLLPSSGKGSVGGHDIMTGAEEIKKNIGYMSQKFSLYDDLTVKENIR
                     FFSGMYGVRGEKRKKRMEWTLEMAGLNDRRDVIVGELPGGFKQRLALGCAILHEPPIL
                     LLDEPTSGVDPLSRRNFWDLINEMSGSGTTVFVTTHYMDEADYCHRLALIYRGRIIAE
                     GTPMELRERYMKRHVMEIEVESMVEALDILERKGMDAAIFGSLLHATVEDVAQGIADI
                     RKTLESADIKVNRIEHIVPSLEDVFVTLIEVA"
     gene            6141..7253
                     /locus_tag="VMU21_08990"
     CDS             6141..7253
                     /locus_tag="VMU21_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO77697.1"
                     /translation="MKFLRIKAIAKKELIQIWRDPLSLAIALLMPVMQLFIYGYAITF
                     DVNNITTIVYDQDRSSLSRELVAQFRESGYFTLVADVDNYKDIDAYLDSNKARVAITI
                     PYDFSKKVLTGRNAHVEVIIDGSDSNTATIAEGYIAAISLRYPQRVTTKTITPVMDPR
                     TRVWFNPELKSRNFIIPGLIAVIMSVVVALLTSLTVAREWERGTMEQLISTPVKTSEL
                     IIGKLTPYFAIGFMDMILAVLVGVTLFGVPLRGNIVLLVALSSVFLFGTVSFGVLISI
                     IAKGSQSVASQIAILSTMLPAFLLSGFMFAISNMRKPLQVITYIFPARYFVTILKGIF
                     LKGSTLTLLWSNGLMLAIASALVFFLAIKNFHKRMY"
     gene            7256..>7589
                     /locus_tag="VMU21_08995"
     CDS             7256..>7589
                     /locus_tag="VMU21_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO77698.1"
                     /translation="MRERITQMIVKEFLKTLRDRRRWMLLFGAPILQLFLFGYVVTTD
                     VNNISTALYDLDKSQESRELARRLEASGYFVVRYAAQSPGEIRDLIDRGEVLCAIQIN
                     RDFAKDLKR"
BASE COUNT         2000 a         1750 c         2147 g         1692 t
ORIGIN      
        1 gggaaggctg aatagggaag gggtgatgag gctcgcggaa gcactgcaac ggcatctcac
       61 cgaacccctc ctcgatgccg ttgcagaaga atccgatacc tttacccgaa gttttttgct
      121 ctctgtgctt gtcgggttca agggcaccat tctcccccat gctgcgagga gaataaatga
      181 caagcgctgg tatgtggtac gtaacgtact ctatctcctt agagagtgcg gcggtgcgaa
      241 ttatatgcac atagtcaggc cgatgacaaa acatgcggac aggagggtga gcatggaggc
      301 attgaaaacc ctccttagtt tcaatgcgcc cggtgccttt tcctacctga ggatatgtct
      361 ggagtccagt gaaccggaag tgagggatca ggcagtgaaa cttgccggaa ccacaagggt
      421 taaagaggct gtgccatacc tccttcaact cctcgaaaag agagacttgg tgggaattga
      481 attggagcaa aaggcatcca ttataagagc attgggggat ataggagacc cgagggcagt
      541 ggataccctt gagaggcttt atagggcaaa aagtctgctt ttcaggaacg caatggagga
      601 actgaggctt gagatagtca ggagcctccg gaactatccg tctgagtcgg tgacaccact
      661 ccttgagagg ggtgcaaagt ccgaaaacaa tgagattagg aagataagtg aagaaatcct
      721 gaacagggaa aaggattgac atgtcccaga aaaaggcaaa cggattcata tcacttataa
      781 tgactgccct ctccaactgt gccttgtatt cagaggatca tcccttagtc agagactttt
      841 ctgtgaaggc tcacggtgaa ataggaggcc tctatgtaaa tgactctttg gctcttaccc
      901 tcctcgggga cagtctggtc tttaacgaga ctcccctcac cgacgcaggc cctcacctac
      961 acagctttac caagaaactg aagaggaaag ggatagaaaa gatcgtcatt gagaaaggag
     1021 tgacctctga cgaaataaag aagtttgtcg cggcgattgc ttcaacagaa aaggtcatct
     1081 ccagccccca tatttcggta gggatggttg aggtgaagtt cggggctgag ggagacgatg
     1141 tcagtacaat tgtcagggag aacattgcta aggtaaaagc cctctttcaa gacgtgaaca
     1201 agttcagaag actggatatg gtgagtcttg aggatgcggt gatagcgttt attgcaacgc
     1261 tgaaaagaga gatgaacgtg cttcgcgtca taagcccggt gaaatcttac agcgagtaca
     1321 cctatgttca tgccacaaat gtatcggtta tcaccctatt ccaggctgag gccctgggcc
     1381 tgaaggggga gaaccttcat gatgtgggta tcgcggcgct catgcacgat gtggggaaga
     1441 tgttcgtcgc caaggaagta cttgagaaac cggggaaact tgacgacaag gaatgggatc
     1501 agatgaaaca gcatccagcc gcaggcgcaa aatacctctc cgcgcttccc gatgttccaa
     1561 aactctccat gattacggct ttcgagcatc acatgaaatt cgatggcagc ggatatccta
     1621 gcacaaaaag gcagagcaaa aagcagcata tcgtcagcca gatggttgcc atcgctgatt
     1681 tctttgatgc cctgagaaca gaaagacctt atcggaaggc catggatatc gctaccataa
     1741 taggacttat aaaagaggca acgggaaagg actttaatcc gctcctcgtg gagaatttct
     1801 tcagcgcgtt aaagcagatt aaagctgctt agcgcgggag agcgcctgtg tggcaagcgc
     1861 gcagttttcc gcaggggcaa ggcaggtggc atcgccggtg ctgtaatgga aaagacctct
     1921 tccggccagc tccctgttgc acgcatcgat acatgccccg caattcacac acgaaacgtc
     1981 ccttttgttc tttctcggga ggacattcat gaagcatgcc ttctcacact ttttgcagtc
     2041 agtacactct ccaatccttg cggtatccag cttcagccgc agggagacag ggcttaccca
     2101 tccaaagagc atctgcatta gtcctgcggc gcagacatat ttgcagaatg tgtggcgtac
     2161 gaaaatggac cctatgagca tatagacggc aacaccgatg atccctgcct ttacgccaaa
     2221 ggtgaagtcc ccgtgcgtga cctggtacca cactgtcttg ggtgccacaa aatagccggt
     2281 gagggcaatc ccgccgcaaa gggggatgag aacaatgctt aagaccgcaa caaggagcca
     2341 aaaaggctta ttgggttttc ccttatcagg gtcattctct cttttgccgt agaggcttct
     2401 ccttcccaga agcttgactg tcagaaagtc agcccattca aagagcgcgc cttcgggaca
     2461 gagccaaccg caaaaggatc tcccggtaag gaagccgagg agcggaaata ccgataggag
     2521 aaggacccag ggcagtattg ccttcaggaa aaaatggagc gccacatgag ttgctcctga
     2581 aatagagtga tctgcataga agccctgctt caacccgaga ctccatacct ggcccaatac
     2641 gatgagttct tttgttgagg agtcgtaacg gaatatgttg agcacaggca tcaggaatat
     2701 gaagaggata aatgtgatct gtgcgatcct cctgtaggtg cccaggtgat gggcgtagcc
     2761 cgaagatgac ctgctgcagg atatgtcgtg ttccattgct gaagagttat ccatgaattt
     2821 ctcctaccat cattgtgtta agtataaggt accattgcgt gagaaatact ggtatgagat
     2881 aaatcataag ggttttgggg gatgctgcct gccataaatg gaggggcggc aggcagcatg
     2941 ggagaaagat tctataagct tagcttatcc ctttggtgca tgactgtata tgatatagat
     3001 catatagaca gtagacaggg aaaatagtaa aactgttttg tggattaaga gaagaaattg
     3061 tgggataatc tccaaagaat gtgcataaga gggattgaga tagggatatc cacggaggca
     3121 ccaggatgag aaagacgttg ctggtccttg gcattctttt ggtggtgggg cttggggtct
     3181 ttctgttgtc ttatctccgc gaaccccggg aagagggggt gcttgccttg tccggcaatg
     3241 ttgaggtgac cgaatatgat atgggattca agttctcggg aagggtcgag aagctgttca
     3301 cggatgagag cctgaaggtg accaagggag atcccctggc tgcccttgat agcgcggaac
     3361 ttgagagcca ggtcagccag gccagggcgt atgtgaagga ggccgaggtc cgccttaaag
     3421 agcttaaggc cggctcaaga ccccaggaga ttgagcaggc gagggcaaac ctcaggtttg
     3481 tggaagcgga gttagaaaag gtgcggaaag acttcgagag agcggaacgg ctctttcgcc
     3541 aggatgcacg gtttcccagt ttgacgctgc aaagagcatg tacgactccc gtgtcgcaca
     3601 gcaaaagagt acacaggaac tcctgagcat ggtgaaggaa ggcccgcgga aggaagagat
     3661 ccttgctgca gaacagcgtg tcagttaggc tcgggcagcc ttgctcaacg tagaagaaaa
     3721 attgaaggac accgtgatat acgcaccgat ctccggggtg gttataagga agaacatcga
     3781 gttgggtgag accgtgggaa ccggcatccc ggtctttacc ttgggagatc tagagaaccc
     3841 gtggatgaag gtgtatgtga aagaggacaa gcttgggctt gtgaaacttg gtcaaaaagc
     3901 aaaggtcacc gttgattcat atccgggaaa ggtatacgaa gggactgtga ccttcatatc
     3961 gtcagaggct gagtttaccc ccaagaatgt ccagacaaag gaggagaggg tgaagctggt
     4021 gttcggggtg aaggtgaggg tgaaacgtca atgatgagct taagcccagc atgcccgcag
     4081 atgtaaagat actgctcaga tgaaccatgg gcggggaagc tattctccac tatgtgacgc
     4141 catccgtgaa gcccctttct cttcggacaa aatcattagt ccggctgcga tgtattcctc
     4201 tggatagtac acccggcctt tggccgagca tccgctctcg ggcagatgca tataagggcg
     4261 ttcaataatg ggcaacgtag cagtaagagc atcgaagctc acaaaactgt ttggcaccac
     4321 tcccgcagtg gacgccctcg gcctcgagat cagaaaaggt gaactgttcg gtctcgtggg
     4381 acctgacggc gggggaaaga caaccatcct gagacttctc accgcaatac tgcaaccctc
     4441 ttccggagag gcatgggtcg caggatattc tgtgattcat gaaggagaaa ggatcaagga
     4501 gaagataggg tatatgtccc agaagttcgg gctgtacgag gacctcacgg tcatggaaaa
     4561 tatcatgttt tacgctgacc tctatgcagt tcctgcaggc gagcggccaa agaggatcga
     4621 acggctcctt ggattcagca atctcacgcc tttcacaggg aggctcgcgg gaaagctctc
     4681 agggggaatg aagcagaaac tgggcctcgc gtgcgccctt atccataccc cggaggttct
     4741 gtttctggac gaaccaacca atggcgtaga ccctgtatcg aggagggatt tctggaggat
     4801 actgtatgac ctcctgaaag agggggtgac cgtggtgatc tccacttcct atctcgacga
     4861 agcggaaagg tgcacaagga tcggactcat tcacaagggg aagatcctca tggaagacat
     4921 tcccacggag atcagaaaat ccctcagaac gaacatggtg gaggtctgga cagatgactc
     4981 ccggaaggct gcggacctca tcaggaaggg tgagggtatt aggggtgtga gcgtctatgg
     5041 ggaccggctc catatagccc ttgtgaacaa agatctctta gacagcatct tccagaccct
     5101 ccaagaagaa gggatcatag tgagggacca cagggagatc ctgccttcca ttgaggatgt
     5161 cttcatttcc atggtggaaa aacaatgaac cagattgaca gtgcagtaaa agtcgaggga
     5221 ttgacccgca ggttcgacga ctttgtggca gtgaatggaa tcaacctgga cataggcaag
     5281 ggtgagattt tcggattcct cggaccgaat ggcgcgggca agtcaacgac cataaagatg
     5341 ctgtgcgggc tcttgctccc cagcagcggg aaaggctctg tgggtggtca tgacatcatg
     5401 accggggctg aggagatcaa gaagaacatc ggctacatgt cacagaagtt ctcgctctat
     5461 gatgacctca cggtgaagga gaacatacgc ttcttcagcg gcatgtacgg ggttcgcggg
     5521 gagaagagaa aaaagcggat ggagtggaca ctggagatgg ccggacttaa cgataggagg
     5581 gatgtgattg tgggagaact gccgggaggc ttcaaacaga ggcttgctct gggctgtgcc
     5641 atcctccatg agcctcccat acttctcctt gatgagccca cctctggggt tgatcccctt
     5701 tcgaggcgca atttctggga cctcatcaat gagatgtcag ggtccgggac caccgtattt
     5761 gtcaccaccc actatatgga cgaggcggat tactgccaca ggcttgccct tatttaccgg
     5821 gggaggatta tcgctgaagg cacaccgatg gagctgagag agaggtacat gaagcggcat
     5881 gtcatggaga tagaggtcga gagcatggtg gaagccctgg acatattgga gagaaaggga
     5941 atggatgcgg cgatctttgg aagccttctc catgcaacgg tggaagatgt ggcgcagggg
     6001 attgcggata tccggaagac cttggaatcg gcggacatta aagtgaacag gatcgaacac
     6061 atcgtaccat cccttgagga tgtatttgtg acgctgatag aggtggcgtg aggggagagt
     6121 gagtttgggg ttagtctaag atgaaattcc tgaggataaa ggccatcgca aaaaaagagc
     6181 tcattcagat atggagggat cccctgagcc tcgccattgc cctgctcatg ccggtcatgc
     6241 agctctttat ctacggctat gcaatcacct ttgatgtaaa caacatcact accattgtct
     6301 atgatcagga caggagcagc ctgagcaggg agctggtggc ccagtttagg gaatcaggct
     6361 attttaccct cgtggcagat gtggacaact ataaagacat tgatgcctat cttgattcga
     6421 acaaggcgag ggtagccatc accatcccct atgacttctc caaaaaggta ctcacgggga
     6481 ggaatgcaca cgtagaggtg atcatcgatg gaagcgattc aaacaccgcg accattgctg
     6541 aaggatatat cgctgccatc tccctcagat atccccagag ggttaccaca aaaaccatta
     6601 cacccgtaat ggacccccgc acccgcgtct ggttcaatcc tgaactgaaa tcgaggaatt
     6661 tcatcatacc cggtctgatt gcggtcatca tgtccgtggt ggtggcactc ctcacctccc
     6721 ttactgtggc aagagaatgg gaaaggggta ccatggagca gttgatatcc actccggtaa
     6781 agacgtctga gctgatcata gggaagttga caccctattt cgccataggg ttcatggaca
     6841 tgatcctggc ggtacttgtg ggtgtcacgc tgttcggtgt tcctctcagg gggaatattg
     6901 tgctccttgt ggccttgtcc agtgtgttcc tgtttggaac cgtttccttt ggggtgttaa
     6961 tctctattat cgcgaagggg tcgcagagcg tggcaagtca gatcgctata ctctccacca
     7021 tgctccctgc gttcctgctt tcaggtttca tgtttgccat ttcgaacatg cggaagcccc
     7081 tccaagtcat cacctacatc ttcccggcac gatattttgt cacgatcctc aaagggatat
     7141 tcctgaaggg aagcaccctg accctcctct ggagtaacgg actcatgctg gccattgcaa
     7201 gtgccctagt gtttttcctc gcaatcaaga attttcataa gaggatgtac tgaacatgcg
     7261 ggaacgtatc acccagatga tagtgaagga attcctcaag acattgcggg acaggaggag
     7321 gtggatgctg ctcttcggag cacccattct ccagctcttc ctcttcggct acgtggtcac
     7381 cactgatgtc aacaacatat ctaccgcact gtacgatctc gacaaatccc aggaaagcag
     7441 ggaactggca aggaggcttg aggcatccgg atactttgtg gtgcggtacg ctgcccaatc
     7501 tccaggcgaa atcagggacc tcattgaccg tggggaagtg ctctgcgcga tacagatcaa
     7561 ccgggacttt gcaaaggacc tgaagagac
//
LOCUS       DATKOU010000194         6810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1046810, whole genome shotgun sequence.
ACCESSION   DATKOU010000194 DATKOU010000000
VERSION     DATKOU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046810"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..2733)
                     /locus_tag="VMU21_09000"
     CDS             complement(31..2733)
                     /locus_tag="VMU21_09000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO77699.1"
                     /translation="MEKQGEFPAIRVILRDGQSLKVRPIEPQDREKLRDFFYRLSPQT
                     RYFRFGYMKTYLSDDELTYYTEMTPPDTFAYVALMGEKDEERIVAVGRWFLAQDTKTA
                     EIAFVVEDNIQVKGIGTALLEQLADAALAYKIKKFIAVVLPENTRMLEVFEESGFKIQ
                     KRIDEGAYQIIFDLEGQEEYAKRQAYREHIARSAGVRTILFPRSVAVVGASRESGSVG
                     WKIFHNLLYADFAGTIFPVNPRATSVGGVLCYPSVSEVPGDVDLAVIVVPAPKVLEVI
                     DQCGKKGVRGLVIISAGFSETGPEGKERQEVLRGKILSYGMRCIGPNCLGIINTDPGV
                     NLNATFAAVAVPRGTLSIGSHSGALGLALLDYVKSNSLGIAHFASIGNRIDISSNDLL
                     EFWKDDENTRVILLYLESFGNPRKFSRIARSVSRKKPIVAMKSGRSEAGRRAASSHTG
                     ALAASDVAVDALFRQAGVIRVNTIEEMFNVAKGLAHQPLPKGSRLAILTNAGGPGILA
                     ADAAVGWGLTVPTLSDDTRKKLAEFLPKEASLSNPVDIIASATGEHFGKSLGILMDEQ
                     DIDAIIIINVPIRPSEEIADAIREAMSLHQCGKTVLACFMMSEAKGIDLSYDSGKRVP
                     VYLFPEDAVQALSHAYPYAQYRGSDEGHIVAFPDIDEERARKLLQTEGLLEGEGGWLT
                     PDSAMRLLKEYGIPVAYTRAAFSADEAASVARAAGFPVAMKLRSRTITHKTDVGGIFL
                     GLEGEDEVKNAYHELMTRLAERGHISEMEGVVVQPMMEGGQEVIIGMSHYPVFGPLVM
                     VGLGGIHVELLKDVAFSLHPLTDKDPDYMFRQLKGLPLLEGWRGSTPKDTEALKEVLL
                     RFSALIDDFPEIAEMEVNPLMVFNRGNGCMAVDARILFKPSTVQ"
     gene            2855..4084
                     /locus_tag="VMU21_09005"
     CDS             2855..4084
                     /locus_tag="VMU21_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HUO77700.1"
                     /translation="MELFKTETVREPLAYRMCPRTIEEYVGQEHLLGEGKLLRRAVEA
                     DRITSLILYGPPGTGKTALARVIAAKTKAHFEWLNAATAGLDDLRKIIQASRARKAKG
                     VPTIVFLDEIHRFNKLQQDALLPDVEEGNIVLIAATVENPFFYVNSALLSRSQIFELK
                     PLSEEALLGILHNTLRDKERGLGNMEIAADEHALMHIARMSDGDARKALSALEIAVLT
                     TPDEKGKVTITLEIAEESIQKKVVVYDKKGDQHYDTISAFIKSMRGSDPDAAIYYLAK
                     MIYAGEDPRFIARRILICASEDVGEADPMALVIATSVLRAVEFIGMPEARIPLAQATI
                     YIAKAPKSNACYRAIESAMEDMGKEQTLEVPDHLKDSHYPGAKKLGHGEGYKYPHDYG
                     GHVEQDYLPGKKKYYNP"
     gene            complement(4122..4595)
                     /gene="rnhA"
                     /locus_tag="VMU21_09010"
     CDS             complement(4122..4595)
                     /gene="rnhA"
                     /locus_tag="VMU21_09010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HUO77701.1"
                     /translation="MEKTRKPFVEIYADGACSGNPGVGGFGALLRANEKERELSGCEE
                     LTTNNRMELLGVICALEALKRPCSVRITTDSDYVVKGMTKWMDAWIRKNWVNSQKKNV
                     LNRDLWERLLRASQPHEVEWVWIKGHNGHPENERCDRLARKEITRCKQRMKGGEV"
     gene            4746..6542
                     /locus_tag="VMU21_09015"
     CDS             4746..6542
                     /locus_tag="VMU21_09015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HUO77702.1"
                     /translation="MKQGAWNLTPLFESDDDPGIEEKRSLLEQKSYTFINTWQDRKDY
                     LEDPDALRKALDEYEAWNRDYGTDGDEGYYFMLRSQQDQINPALKASYNKIEEFGQKI
                     QNDIQFFHLSIGRIPLEKQTQFLKYHGLKPYRHFLERLFAESRYMLSEAEEKILNLKS
                     PTSYFNWVKMTSGFLSKEERMVLSEEGKKSSQTFSEIMSLMNSKTKKVRDSAAKAFNY
                     IMNTHVDVAEAELNSILANKKVDDELRKMDRPDLARHVSDDIESDIVDSLLDAVSMRF
                     SISSRYYELKAKLMGVKKLKYHERNVEYGDVVKRYPFKKSVRLIEKVLGNLDSEFLEI
                     LREFLQRDQIDIYPRKGKASGAFCAHHLISQPTFILINHTGKLYDVLTLAHELGHGIN
                     NELMRKRQNALNFGAPLSTAEVASTFLEDFVLQELLKEADDELRLALMMNKLNDDVST
                     IFRQVACYRFEQSLHGEFRQKGYLSKEEIGKLFCDNMSAYMGEFIEQSPGSENWWVYW
                     GHIRSFFYVYSYASGLLISRSLQNSVKENPLFIARVKDFLSAGLSDSPRTIFRNLGID
                     IADKGFWEKGLNEIENLLHETTLLARKLGKLK"
     gene            complement(6548..>6810)
                     /locus_tag="VMU21_09020"
     CDS             complement(6548..>6810)
                     /locus_tag="VMU21_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77703.1"
                     /translation="MDQKLAIGIDQVNRLLESMVKTQTAVEQQTSQTDDLLREAINKR
                     FDALSEEILASDQFREELLRLKEDILKEVEKHYQARPAPSKK"
BASE COUNT         1728 a         1818 c         1600 g         1664 t
ORIGIN      
        1 tcctttctta gtgacgaggc ttttcaccac ctactggacg gtagatggtt taaataatat
       61 ccttgcgtca acagccatgc agccgtttcc cctgttaaaa accatgaggg ggtttacctc
      121 catttccgca atctcgggga aatcatcgat aagggcagag aaacgcagaa gcacctcttt
      181 cagcgcctcc gtatccttgg gagtactccc gcgccagccc tcaagcaggg ggagaccctt
      241 cagctgtctg aacatgtaat cagggtcctt atcggtaaga ggatggagtg agaaggcaac
      301 atccttcagg agctctacat ggattccccc cagtcccacc atcacgaggg gcccgaatac
      361 gggatagtgg gacatgccga taatcacttc ctggcctccc tccatcatgg gctgcaccac
      421 aacaccttcc atctcagaga tgtgtcctct ttccgcgagg cgagtcatca gctcatggta
      481 tgcgttcttc acctcgtctt ccccctccag tcccagaaag attcctccca cgtcggtctt
      541 gtgggtgatt gtcctggagc gaagtttcat tgccacagga aaacccgcag cccgtgcaac
      601 actggctgcc tcatcagcac taaacgcagc ccttgtatac gcaaccggta tcccgtactc
      661 cttcaaaagc ctcatcgcac tgtcaggggt taaccagccg ccctctccct caagaagccc
      721 ctcggtctgg agaagcttcc tcgccctctc ttcatcaatg tcgggaaagg cgacgatgtg
      781 tccctcgtct gaaccacggt actgggcata gggataggca tgggagaggg cctgcaccgc
      841 gtcttcagga aaaagataga ccggtacacg ttttcctgaa tcgtaactga ggtcgatccc
      901 ctttgcttct gacatcataa aacaggcgag cacagtcttg ccgcactggt gtaaagacat
      961 tgcctccctg atcgcatccg ctatctcctc agagggtctg atgggtacat taatgataat
     1021 aatcgcgtcg atatcctgtt catccatgag gatccccagg gatttgccga aatgttctcc
     1081 tgttgcagag gcgatgatat ccacaggatt ggagagggac gcctcctttg ggaggaactc
     1141 ggcgagcttt tttcgcgtgt catcagaaag ggtgggtacg gtaagccccc accctaccgc
     1201 agcatccgca gccagaatcc ccggaccgcc cgcattggtg agaatggcga gccgcgatcc
     1261 cttgggtaat ggctgatggg caagtccctt tgccacattg aacatctctt ctatggtatt
     1321 cacccttatc actcctgcct gccgaaaaag ggcatccacc gcaacatctg atgctgcaag
     1381 cgctccggta tgggagctcg ccgcccttct ccccgcctcg ctcctgcctg acttcatggc
     1441 tacgatgggt tttttgcgcg aaacgctcct cgcgatacgg ctgaatttcc ggggatttcc
     1501 aaagctctcc aggtagagga gaatcaccct tgtgttttca tcatccttcc agaactccag
     1561 aaggtcgtta gacgaaatat caatgcggtt accgatgctt gcgaaatgtg cgatgcccaa
     1621 gctattgctt ttcacataat cgaggagtgc gagccccagg gcaccactgt gggatcctat
     1681 gctcagggtt ccccggggaa cagcgacagc cgcaaaggtg gcgttcaggt ttactccagg
     1741 atcggtattg ataatcccca gacaatttgg ccctatgcac cgcattccat aagaaaggat
     1801 tttccccctg agaacttcct gtctctcctt accctccggg cccgtttcac tgaatcccgc
     1861 agatatgata accagtcccc tcaccccttt tttcccacac tggtcgatca cctccaatac
     1921 cttgggggca ggcaccacta tcactgcgag gtccacatcc ccggggacct cgctgaccga
     1981 aggataacac aacacccctc ccactgaagt tgctctggga tttacaggga aaatagtacc
     2041 agcgaagtcg gcataaagaa gattatggaa tatcttccac cccacgcttc cagattccct
     2101 ggatgcgccg accactgcaa cgctccttgg aaataggatt gtgcgtaccc cagcagaacg
     2161 ggcaatgtgt tcacggtatg cctgacgttt cgcgtactct tcctggcctt caagatcaaa
     2221 aataatctgg tatgcgccct catcgattct cttttgtatt ttaaaaccgc tctcctcaaa
     2281 gacctcaagc atgcgggtgt tttccggaag cacaactgcg atgaatttct ttattttgta
     2341 cgcaagggct gcatccgcaa gttgttcgag aagggcagta ccgatcccct tcacctggat
     2401 gttgtcttcc acaacgaagg cgatctctgc ggtctttgta tcctgggcaa gaaaccatct
     2461 gcccaccgcc acaatcctct cctcatcctt ttcccccatg agtgccacgt acgcgaaagt
     2521 gtcaggggga gtcatctctg tataataggt gagttcatcg tcactcaagt aggtcttcat
     2581 atacccgaaa cggaagtaac gcgtctgggg gctcaagcgg taaaaaaagt ccctcagttt
     2641 ttccctgtcc tgaggttcaa tgggtctcac cttgagactc tgtccgtccc tcagtattac
     2701 ccttattgcc ggaaactcac cctgtttttc catgactatg cccttccctt tccttctctt
     2761 ttatcagggg cctcattcat taccacatta tctttaagta tacactcatt cggtgcgtct
     2821 ggacatatct tgtgcaaaac ggttagattg atacatggag ctctttaaga cagaaacggt
     2881 gagagagccc ctcgcgtatc gcatgtgtcc cagaacaatt gaggaatacg tggggcagga
     2941 acatcttctt ggggaaggta agctcctgag gagggcggta gaagccgata gaatcacctc
     3001 cctcatcctt tacggacctc caggtacagg gaagaccgca ctggccaggg tcatagccgc
     3061 aaagacaaag gcccactttg aatggctcaa tgcagccact gcaggcctgg atgaccttag
     3121 aaagatcatc caggcctcac gcgcgcgtaa ggcaaaaggt gtcccaacca ttgtcttcct
     3181 cgacgagata caccggttta acaaactcca gcaggacgcc ctcctccctg atgtggagga
     3241 agggaatatt gtgttgattg ccgcgaccgt tgaaaaccct ttcttttatg tgaacagcgc
     3301 gcttctttcc cggagccaga tctttgaact gaaaccatta tcagaagaag cactcctggg
     3361 tatcctgcat aacaccctac gggataaaga gaggggtctc ggaaacatgg agatagccgc
     3421 tgacgagcac gcccttatgc acattgcgag aatgtctgat ggcgacgcga ggaaggctct
     3481 ctccgcccta gaaattgctg tcctcaccac ccctgacgaa aaggggaagg ttaccattac
     3541 ccttgagatc gctgaagaat ccattcagaa aaaggtggtg gtgtatgaca agaagggtga
     3601 ccagcattac gacaccatct cggcctttat aaagagcatg aggggctctg atcctgatgc
     3661 cgccatttac tatcttgcga aaatgattta tgcgggagaa gaccctcgtt ttattgcgcg
     3721 aaggatactc atatgcgcgt ccgaggatgt gggagaggct gatcccatgg cactggttat
     3781 cgcaacctct gtattaaggg cagtcgaatt cataggaatg cctgaggcga gaatacccct
     3841 tgcacaggcc accatctata tcgcaaaggc ccctaaaagc aatgcctgtt acagggcaat
     3901 agagtcggcc atggaggata tgggaaagga acagaccctt gaggtccccg accatttgaa
     3961 ggattcccac tatccaggcg caaagaaact gggacatgga gaaggataca aatatcccca
     4021 cgactacggc gggcatgtgg aacaggacta cctgccagga aagaagaaat attacaatcc
     4081 ctgacctctc cagaaataaa gaaactcatg aataaggtgg gtcagacctc tcctcccttc
     4141 atcctttgct tacagcgcgt tatctccttc cttgccaacc ggtcgcaccg ttcattttcg
     4201 ggatgcccat tgtgtccctt tatccagacc cattccacct catggggttg tgatgccctc
     4261 aaaagccttt cccacagatc cctgttgagc acgttctttt tctgggagtt cacccagttc
     4321 tttcttatcc atgcatccat ccacttggtc atccccttca ccacataatc ggaatctgtg
     4381 gtaatcctta cgctgcatgg tctcttgagc gcttcaaggg cgcaaatcac tcccagcagc
     4441 tccattctgt tgtttgtagt caactcctcg cagccggaaa gttccctctc tttctcgttc
     4501 gctctcaaga gcgcaccgaa cccgccaacc cccgggtttc cgctgcatgc gccgtccgca
     4561 taaatctcca caaatggttt ccgagtcttt tccaccgttt attattttac attgtttcgc
     4621 atgacagttc caaaccagac aatccttcgg tgctataata ccctatggtt cctcccctga
     4681 gttggttgag gagggaacct taacttcgtt atacccgatg taaggggacc agaaaggaag
     4741 tggacatgaa acaaggcgca tggaacctta cccccctctt tgaaagcgat gacgacccgg
     4801 ggatagaaga aaaaaggagc ctcttagaac agaaaagcta tacattcatc aatacatggc
     4861 aagaccgcaa agattacctc gaggatcctg atgcgctcag aaaggcattg gatgaatacg
     4921 aggcatggaa cagggattac ggcaccgatg gtgacgaagg atactatttt atgctcagat
     4981 cacagcagga ccaaattaat cctgccctta aggcgagcta taacaaaatc gaggagttcg
     5041 gtcagaagat tcagaatgac atccaattct ttcatctcag cataggcaga atccccctgg
     5101 agaagcagac acaattcctt aaatatcatg gattgaagcc ctacagacat ttcctggaaa
     5161 ggctttttgc agaatcgagg tacatgctca gcgaagcaga agagaagata ctgaacctca
     5221 agtcacccac ctcttatttc aactgggtaa agatgacctc cggattcctc tcaaaggaag
     5281 aaagaatggt gctttccgag gagggcaaga agagttcgca aaccttttct gaaatcatga
     5341 gtctgatgaa cagtaagaca aaaaaggtga gggattctgc ggcaaaggct tttaactata
     5401 tcatgaacac acatgttgac gttgcggaag cagagttaaa ctctatcctt gccaataaga
     5461 aggtcgatga tgaacttcgc aaaatggacc ggcctgacct ggccagacat gtgagcgatg
     5521 atatcgaaag tgacattgtt gattccctcc ttgatgcggt ctcaatgcgt ttttccattt
     5581 catcacggta ttacgaactg aaggcaaagc tgatgggagt aaagaagctg aaataccacg
     5641 agagaaacgt ggaatatggc gatgtggtta agcgctatcc ttttaagaaa agtgtaaggc
     5701 tcatcgagaa ggtccttgga aatctcgaca gcgagtttct ggagatcctt cgcgagtttc
     5761 tccagcgtga tcaaattgat atctaccccc gcaagggaaa ggcgagcggc gctttctgtg
     5821 cccatcacct catttcacag ccgaccttca tccttatcaa tcacaccgga aaactgtatg
     5881 atgtgctcac actcgcccat gaactaggtc atggaataaa caatgaatta atgaggaaaa
     5941 gacagaatgc ccttaatttt ggcgccccgc tttccacagc cgaagtggca agcaccttcc
     6001 tggaagattt tgtcctccag gaactcctca aagaggctga tgatgaatta cgcctagccc
     6061 tgatgatgaa caagttgaat gatgatgtat ccaccatatt caggcaggtg gcgtgctata
     6121 gatttgaaca aagtctccat ggggaattca gacaaaaggg ctacttgtcc aaggaagaga
     6181 tcgggaagct cttctgcgac aatatgtcag catatatggg tgaattcatt gaacaatctc
     6241 ctggatcaga aaactggtgg gtgtactggg gacatatccg gagtttcttt tatgtatatt
     6301 cgtatgcgag cggcctcctc atctcaagat cacttcagaa ctccgtaaaa gagaatcccc
     6361 tgttcatcgc gagggtcaaa gattttcttt cagcaggctt gtccgattct cccaggacca
     6421 tcttcagaaa ccttgggatc gatatcgcag acaaagggtt ctgggaaaaa gggctgaacg
     6481 agatcgagaa ccttttgcac gagaccacct tattagccag aaaattagga aagctaaaat
     6541 aaggagctca tttttttgaa ggcgctggtc gggcctgata gtgcttttca acttccttaa
     6601 gaatatcctc cttcagcctt aagagctctt ccctgaactg atcgcttgcg agtatctcct
     6661 cggacagtgc gtcgaacctt ttgtttatcg cctcacgcag gaggtcatcc gtctggcttg
     6721 tctgttgctc aacggcagtt tgggtcttta ccatggattc gaggagccgg tttacctgat
     6781 cgatgccgat ggccagtttt tggtccatta
//
LOCUS       DATKOU010000195         5043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3734417, whole genome shotgun sequence.
ACCESSION   DATKOU010000195 DATKOU010000000
VERSION     DATKOU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3734417"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VMU21_09025"
     CDS             <1..130
                     /locus_tag="VMU21_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HUO77704.1"
                     /translation="LVERFFAWLGWQRRLLTRWEYHAENFLGFVRLASIKILLNRF"
     gene            complement(590..1168)
                     /locus_tag="VMU21_09030"
     CDS             complement(590..1168)
                     /locus_tag="VMU21_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77705.1"
                     /translation="MADTKYCLCCGVDVPFNYVEREDRREITCAYCGFTLDVEKSEKS
                     SDVREGYVLITDDSKNVRDIIVDALKETKIYKNIMTFENGLELTTSFSYLVSRKSPID
                     VAIIDLVMPIMDGFTAANTLRAIEFQYKISATPIIFFSAVQADDSFREKMELLSPAIY
                     LNKGADPEPGSLARRVKQLVEILTTKNRETPH"
     gene            1340..2722
                     /locus_tag="VMU21_09035"
     CDS             1340..2722
                     /locus_tag="VMU21_09035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUO77706.1"
                     /translation="MKIRFLGGVRTVTGTCFLLQTSQLQVLIDCGMIQGADNGDMNRE
                     SFAFHPSQIEYLFLTHAHIDHTGLVPMLVKDGFSGTIFSSSATADLVELMLYDSAHIQ
                     ESDAELMTRKALRAGRRPVSPLYTVEDVKKVLPLFNRVPYGKIGYTGKGIKYRFLDAG
                     HILGSGSLELWFQDGPQEKKLLFSGDIGKKGNPIINDPTRETEADYVVMESTYGNRLH
                     KGMEETINELREAITLTFRKGGNVIIPAFAIGRTQDILYVLNRLVRERRLSPVNVYID
                     SPLAQEATKTYLAHPECYDEEAKRLLENWDKEDTLRLRFTHTVEESMTLNRIKSNAVI
                     IAGSGMCEGGRIKHHLKHNLWRPECSVIFAGFQAEGTLGRRIVDGAKVVRLFGEEVAV
                     KARIYTLGGFSAHADRKELLEWLSAFRNRPVVFVVHGEEETSLEFSEAVKKQYGFTTY
                     VPLKGESFEL"
     gene            complement(2815..3729)
                     /locus_tag="VMU21_09040"
     CDS             complement(2815..3729)
                     /locus_tag="VMU21_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HUO77707.1"
                     /translation="MTERNHPEQGNMQVVERKNLFEKAVLYLKMIKFPHSVFALPFAL
                     TSAFIAARGIPHGRTLFWIVVAMVSARTTAMGLNRIIDRRIDEENPRTALRELPAGQL
                     TVLETSVFVAIAMVTFELSAYMLNPLCFRLSFIALFFIALYSFTKRFTWASHFVLGIT
                     ISAAPVGAWIAVTGGFETGILLLGFSVVFWLAGFDILYAFQDVEFDRRYGLYSLPLRF
                     GITKSLWISRVCHTCTWVLLVCTGIFFGMTFPYYIGILITALLLIYEHSMVKPDDLSK
                     LNRAFFDMNGYISITVFLFTVLSYAVPR"
     gene            complement(3726..4490)
                     /locus_tag="VMU21_09045"
     CDS             complement(3726..4490)
                     /locus_tag="VMU21_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HUO77708.1"
                     /translation="MNPLEIYKLLPKLNCGECTPKTCMSFAVSLGGNPGSLAQCRHIK
                     QENLDAIKSLLAQGEWKDKLIKSLMDEVSKLGLEEIAPDLGCCMRNDTLVVRCIGQEY
                     SMGGDGTISPDTKNKWINILLLHYVRNRGKGEFTGKWVSFSELKGGFIKSSSFLRECE
                     EPLRELMDQDPKGISSMVARLGAAPAQGFPADHAWTMDLLPKVRALFLYRAGDEEFPS
                     SMKILFDSITGQFLDVESLMFVCEGLIHTLRMMRRG"
     gene            4657..>5043
                     /locus_tag="VMU21_09050"
     CDS             4657..>5043
                     /locus_tag="VMU21_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77709.1"
                     /translation="MKFIGLDAGSVSVKLVVLDEKGSKTQGFYARHKGHPLKVSLELL
                     ETVVSGNVQQRGSETPGSDQFSLSVTGSSGRWIANALGIGFTNEVIAQTYATRKLSPS
                     TKTIIELGGEDSKLIVFGEKGIKDFSM"
BASE COUNT         1373 a         1232 c         1190 g         1248 t
ORIGIN      
        1 gctcgttgag aggttctttg cctggctcgg gtggcagcgc agactcctga ctcggtggga
       61 atatcatgca gagaacttcc tcggatttgt gagactcgct tccatcaaaa tactcctaaa
      121 tcgattttga gataggttct aacagctact agaacttaat ccttatcacg agattgaaaa
      181 tgcccgcagg gcccattgtt tttgtccact ttcttaataa gaaaaccaca attgaaccca
      241 tcccttttat gaggaagaaa gaaaatacag tccgcgcact tttccatctg ttcctcctta
      301 atttatagga atatctacag atgcttaagc aggttttatg cctatcaaat cccttgtaaa
      361 tgcatcgatt attcaggata gcttgcaagc ctccagggaa aaccccaaat tgtgattttc
      421 aaaatgtgtg ataaccccaa attaagacct ggcagcaatt catcataata acagaatgcg
      481 gttgtcactt caacagatcg atgataggca aatcagtctt tatacgtgcg agcgtccctt
      541 gctgaaacca caggacgcat gggattactt aacaaatcag cgtgtactct taatgcgggg
      601 tttccctgtt ctttgttgtg agtatctcca caagctgctt aaccctcctc gcgaggctgc
      661 caggttcggg gtcagcacct ttgtttaaat atattgcggg gctgaggagt tccatcttct
      721 ccctgaagga gtcgtctgcc tgcactgcag aaaaaaagat gattggggta gcagaaattt
      781 tgtactgaaa ttctattgcc ctcaaagtat ttgcagcagt aaagccatcc atgattggca
      841 taacgaggtc tataatggcc acatcaatgg gtgacttcct ggagacaagg tacgaaaaag
      901 aggtggtaag ttcaagacca ttctcaaagg tcattatatt cttatagatc ttggtttcct
      961 taagggcgtc aacaataata tcccggacat tttttgaatc atccgtgatg aggacatacc
     1021 cttccctcac atcggaggat ttttctgatt tttccacatc cagcgtaaaa ccgcagtagg
     1081 cacacgtaat ctccctccga tcttccctct ccacataatt gaagggaaca tccactccgc
     1141 agcaaagaca gtacttggtg tctgccatac ccctgttcct atgggcatta tatatcccgt
     1201 ctgcacatat tgcaaggatt tccccgtcag gcgtttttac aagggacaaa aaatctggta
     1261 tgatttgcag agcatccagg aaactatcac agaactgccc gttaatgaac aaaccctttc
     1321 atcatatcag gatgagctcg tgaagatacg cttccttggc ggggtaagga cggtcaccgg
     1381 aacatgtttc cttctccaga cgtcccagct ccaggtcctc atagattgcg gcatgattca
     1441 gggggcggac aacggagaca tgaacagaga gtcctttgcg ttccatccct ctcagattga
     1501 ataccttttt ctcacccacg cacacattga ccacaccggg ctagtgccga tgcttgtgaa
     1561 ggatggattt agcgggacaa tcttctcctc atctgcaacc gcagaccttg tggagctgat
     1621 gctctatgat tcagcacata tccaggagtc ggatgcggaa ttgatgacaa ggaaggcgct
     1681 ccgcgcaggg agacgacccg tttctccttt gtacactgtg gaagacgtta aaaaggtact
     1741 gccccttttc aaccgagttc cctatggaaa aataggatac acagggaagg gaataaaata
     1801 caggtttctg gatgccggac atatcctggg ctccggaagc cttgagctgt ggtttcagga
     1861 cggcccgcag gaaaagaagc ttctcttttc aggtgatatc ggaaagaagg ggaaccctat
     1921 cattaatgac cccacaaggg aaacagaggc tgactatgtg gtgatggagt caacctacgg
     1981 gaacagactc cacaagggga tggaagagac catcaatgaa ctgcgggagg ccataactct
     2041 caccttcagg aaaggcggca atgtcattat cccggcattt gccataggga ggacacagga
     2101 tatcctgtat gtccttaaca ggctcgtgag ggaaagaagg ctgtctcctg tcaatgttta
     2161 catcgacagc ccccttgccc aagaggccac gaaaacttac cttgcccacc ccgaatgcta
     2221 tgacgaagag gcaaaacggc tcctggagaa ttgggataag gaagatactc tcaggcttcg
     2281 ctttacccac acagtggaag agtccatgac actgaacagg atcaagtcca atgcggtcat
     2341 cattgccggg agcggcatgt gtgaaggggg caggataaaa caccatctga aacacaatct
     2401 ctggaggcct gaatgcagcg tcatatttgc gggctttcag gctgagggaa ccctcggaag
     2461 gaggatcgtt gacggtgcaa aggtggtgcg tctcttcgga gaggaggttg cagtaaaggc
     2521 aaggatttat accttggggg gattttcggc ccacgctgac cggaaagaac tcctggaatg
     2581 gctctctgct ttcaggaacc gtcctgtggt ctttgtggtc catggagaag aagagacatc
     2641 cctggaattt tctgaagccg ttaagaaaca gtatgggttt acgacctacg ttcccttaaa
     2701 aggagaatca ttcgaactgt agttcccgga cttctggtgt ttctttacgt ccttcgatac
     2761 cgctccgtga tttcaccagt ctgacatgac aacttatgga tgagtgccac ccaatcaacg
     2821 cgggacggcg taagataaaa cagtgaagag gaatacagtg atgctgatat acccgttcat
     2881 atcaaagaat gccctgttga gcttgctcag gtcatcaggc tttaccatcg aatgttcata
     2941 gatgagcagg agcgccgtaa tgagaatccc gatataataa gggaatgtca tgccaaaaaa
     3001 gattccggtg cacacgagga gcacccatgt gcatgtgtgg cacactcgtg aaatccagag
     3061 cgacttcgta atgccgaacc tgagaggcag ggaataaagg ccatatcttc tgtcaaattc
     3121 cacatcctga aatgcgtaaa ggatatcaaa acccgcaagc cagaacacta cagaaaaacc
     3181 cagaagaaga atccctgtct cgaaaccgcc tgtaaccgca atccaggctc ccaccggcgc
     3241 cgcggagatg gtgatgccga ggacaaagtg ggatgcccat gtaaatctct ttgtaaatga
     3301 atagagggca atgaagaaca atgcaataaa cgacaaccta aaacagagcg ggttcagcat
     3361 atacgctgaa agctcgaagg tcaccattgc gattgccacg aatacagatg tctccaggac
     3421 cgtgagctgc cctgccggta attcacggag tgcggtcctc ggattctcct cgtctattct
     3481 cctgtcaatg atcctgttca gccccattgc agttgtcctc gcagagacca tcgccaccac
     3541 aatccaaaac agggttctcc cgtgggggat gcctctggct gcgataaagg ctgaggtgag
     3601 ggcaaagggc aatgcaaata cagaatgggg gaacttgatc attttgaggt aaagaaccgc
     3661 cttttcaaaa agattctttc gttccaccac ttgcatgttc ccctgttccg gatggttcct
     3721 ttctgtcatc cccgcctcat catcctcaat gtgtggatga gcccctcaca cacaaacata
     3781 agggattcca catccaggaa ctgtcctgta atgctgtcga agagtatctt catagaagag
     3841 ggaaattcct catcacccgc acgataaagg aacaaggccc tcacctttgg gaggaggtcc
     3901 atggtccagg catgatccgc aggaaaccct tgagcgggag cagcgcccaa tcgggccacc
     3961 atagaggaaa tgcctttggg atcctggtcc atgagttccc gaagaggctc ctcacactct
     4021 ctcagaaatg aggatgattt gataaagccc cctttcagtt cagaaaaaga gacccatttc
     4081 ccggtaaact cgcccttccc cctgtttcta acataatgga ggagcagtat gttaatccac
     4141 ttattcttag tatccgggct gatggttccg tccccgccca tggaatactc ctggccgata
     4201 cagcgaacca caagtgtatc gttcctcatg caacatccga gatcaggggc gatttcttca
     4261 agcccgagtt ttgaaacctc atccatgagg gatttaatga gtttatcttt ccattcaccc
     4321 tgtgcgagca gggactttat cgcatcaaga ttctcctgtt taatatgtct gcactgtgca
     4381 agggaaccgg gattaccccc aagcgagacg gcaaaggaca tgcacgtttt gggagtgcat
     4441 tctccgcaat tcagttttgg caatagtttg tagatttcca atggattcat gtgttcattt
     4501 cctttgtcag ttccatcagg tctccccttt ttcgctgtat gccaaaagtt tacctcagcc
     4561 gcaccacaaa agaaaactgg ttcactgttt tcccgtgata tgataaaatt ataacgtagt
     4621 gtctggcaat ccgaatctta ccaccatgga gaatttatga aattcatcgg tcttgacgcg
     4681 ggctctgtaa gtgtcaaact tgtggtgctt gatgaaaaag gtagcaagac acagggattt
     4741 tacgcacgcc ataaaggcca ccccctgaag gtctctctag aacttcttga gaccgtagtc
     4801 tccggcaacg ttcaacaacg tggcagcgaa actcccggct ctgaccaatt ttccctttcc
     4861 gttaccggtt cctcaggaag atggattgcc aatgcacttg gcattggatt tactaacgag
     4921 gtgattgcac agacttatgc cacgagaaag ctctcgccaa gtacaaagac aattatagaa
     4981 ctgggtggtg aagactcaaa actcatcgtg tttggggaaa agggcataaa ggacttttcc
     5041 atg
//
LOCUS       DATKOU010000196        13450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1433419, whole genome shotgun sequence.
ACCESSION   DATKOU010000196 DATKOU010000000
VERSION     DATKOU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433419"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1281
                     /gene="extKL"
                     /locus_tag="VMU21_09055"
     CDS             376..1281
                     /gene="extKL"
                     /locus_tag="VMU21_09055"
                     /inference="COORDINATES: protein motif:HMM:NF040886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome ExtKL"
                     /protein_id="HUO77710.1"
                     /translation="MKGFRIMLVLLMIIALPVAAAAQKAKTIDELATMYDASSCKSCH
                     AEIYAAWEKSHHARPLMGVKGGLMLTPLALKKGEPGSTAFSEDSPKKMTVKNFPCFKC
                     HLPQAVISAEDSVAVELAEALIAKDSAKVAKLQITCIVCHNTKAIIHKRELGEPEKGV
                     LYGLKDVASHGDKTYTKVKKSAIMKDSVFCGQCHGLGPNLEFDQAFQCANLYGSYLHN
                     YVSSGGTHTCQECHMPKVDGKADHLIAPNFNDDKGTSELLAKAINLDVQTIGYEWLRK
                     AATYVPKIVVETKITTTAGHRIPDG"
     gene            1303..1602
                     /locus_tag="VMU21_09060"
     CDS             1303..1602
                     /locus_tag="VMU21_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77711.1"
                     /translation="MVVTAKSMDGKEIGKVEKHYHPQATNCRDTKERYGAQWKVANLR
                     DTSLQPNKTKAETIEFELPAGVRAADVTVDLVYEDVNPDNKYPIHSITKRVSLDK"
     gene            complement(1669..2748)
                     /gene="ribD"
                     /locus_tag="VMU21_09065"
     CDS             complement(1669..2748)
                     /gene="ribD"
                     /locus_tag="VMU21_09065"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HUO77712.1"
                     /translation="MNRAIMLSTRARGMTSPNPMVGAVIVKNGKIIAEGYHRKPGTPH
                     AEALAITAAGEKTRASTLYVSLEPCCHTDKRTPPCTRTIIDSGIKRVVMAMRDPNPKV
                     SGKGMKKLKRAGIEVVTGVLEEKAKQVNEYYIKYITTRRPFVILKVAMTLDGKIATGA
                     GQSKWITGEKSRMMVHRLRSSVDAVMTAIGTVKADDPQFTARIRNGRNPVRILIDPSL
                     EVSPDAKILRVPPETVIVTKNTRNPALLKKANKLFSMGSYIILYKHTLELNWLMKKLG
                     EKGITSIVIEGGSSLNAHALEDGIVDKVMLFIAPKIMGGTASYPAVGGKTLRDLDEAY
                     RLKNTTVKRLGEDLLVEGYLTSSPA"
     gene            2890..3276
                     /locus_tag="VMU21_09070"
     CDS             2890..3276
                     /locus_tag="VMU21_09070"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HUO77713.1"
                     /translation="MSNILLGLITLSFIVAVVVVIFVMIELRGAIRALKEFIKTTENS
                     LKPTLEELQQTLKSLRNVTDNVTGVTEDVRVLSSSVRDVGENVKHVSELVDEVTSSAV
                     VRVSGLRAGVTAAMEVLLKNIFSGRK"
     gene            3369..3689
                     /locus_tag="VMU21_09075"
     CDS             3369..3689
                     /locus_tag="VMU21_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HUO77714.1"
                     /translation="MRHDEECGHSTGSLFLAFLLGGLAGAGAALLLAPKSGNETRQRI
                     RELAEEVKEKTEDYVEQAKGRVSSTVEKGKGYIQEKKSIVTTAVEAGKEAYEKEKERL
                     AKES"
     gene            3689..4057
                     /locus_tag="VMU21_09080"
     CDS             3689..4057
                     /locus_tag="VMU21_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HUO77715.1"
                     /translation="MHETSIALGILDIISDRCIAEGYHSIESVRLRIGRAAGIMPEAL
                     LFSFDVAKEGTLAESAKLIIESVSLGGVCSECNAEFEVEEAYVLSCPECGSAAFKIQR
                     GYEMEIVEMDVKEEKHNISP"
     gene            4074..4715
                     /gene="hypB"
                     /locus_tag="VMU21_09085"
     CDS             4074..4715
                     /gene="hypB"
                     /locus_tag="VMU21_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HUO77716.1"
                     /translation="MKVKVVTRILEANERLAEENRKRFKEAGVFVMNLMGAPGAGKTS
                     LLEKTILELKTRVRIGVIEGDIAGTDDAERIHNTGVPVVQINTGGACHLDANMISEVL
                     PEMPLGELDMVIVENVGNLVCPAEFKVGEDIKAMVLSITEGHDKPLKYPLMFQESAAL
                     VLNKVDLLPYVNVDMQKLKNDAISLNPKLKIFEVSCKTGEGLHRWTEWLNGLK"
     gene            4694..7075
                     /gene="hypF"
                     /locus_tag="VMU21_09090"
     CDS             4694..7075
                     /gene="hypF"
                     /locus_tag="VMU21_09090"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HUO77717.1"
                     /translation="MVERAKITLKGVVQGVGFRPYVYILAESLQLRGYVTNTPDGVTI
                     DIEGEHVSEFIERLPKDPPPLSQIAAISITPLPEYGYGDFRIIESREESSRVPFTLIS
                     PDVSICDDCLEELMDPHDRRYLYPFINCTNCGPRYTIIRSIPYDRPHTTMANFSMCAQ
                     CNAEYHDPHDRRFHAQPNACPQCGPNVTLVNSRQPELDGQVAMEKTIELLKAGRIIAI
                     KGLGGFHLACDATNKTAVEELRERKRRSNKPFALMAPDLLAVERFCTVSEEEAKLLSS
                     RRRPIVLLRKKEAVSLLPEAVSPHNQYLGFMLPYTPLHYLLFHQPFPAREGGMLSGRE
                     ANFSALVMTSGNLSEEPIVRDNEEALEKLQGIVDAFLLHNRDIFMRVDDSVIRVRYEG
                     PTVPCQEGSILSDKRRESTLFLRRSRGYAPEPLALQSNGPEVMGCGADMKNTFTVTKG
                     GYAIPSQHIGDMENYETVQFYEECLENLKRVCRVKPVAMAHDLHPGYLSTQWALRQGV
                     QTYGIQHHYAHIGSVMAEKGLLGKVLGVAFDGTGYGTDGNLWGGEVLLADVDGFERVA
                     HFKYISLPGGERAVKEPWRTAISYILDAAGDEAMEYLEKIGFLEKFGKERTEKIIKIA
                     GARELSPQSSGAGRLFDAVAALTGVCDINTFEGEAAMALESLVSGGTEEDYPVNIILE
                     KSMTIDFSHTIIGIVNDIVNGTEKHLISTKFHNTVSNVILKTVGRLWETFMLEEVVLT
                     GGTFQNLYLLERTTRMLAATGMKVFINEMVPCNDACISLGQAYLVRERIKNKG"
     gene            7156..8220
                     /gene="hypD"
                     /locus_tag="VMU21_09095"
     CDS             7156..8220
                     /gene="hypD"
                     /locus_tag="VMU21_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HUO77718.1"
                     /translation="MKGMLNDIKRMMDLMGRPLKLMEVCGTHTVAIFRHGIRDIIPRE
                     ISLLSGPGCPVCVTSIKDVDSAIAVAKLPGVILGTFGDMMRVPGGVECLADARAEGAD
                     VRVFYSPMDALKLAMDEKDRQVVFFATGFETTSPLIAATLFDAAKRGVTNFFIYSTHK
                     LVPPALRALLGSPDVMVDGFILPGHVSTIIGKKPYEFLASEYKKPGVITGFDADDIIE
                     GIHMLVSQMLHGDAEVEIQYKDVVREEGNPRAVSLIEEVFTPADAYWRGIGTIPQSGL
                     TLREEFKRFDAIGRFSPEVPYHPEPEACSCGDVLRGVKIPLECPLFGTACSPESPVGA
                     CMVSTEGSCAAYYKYGAYRG"
     gene            8213..9208
                     /gene="hypE"
                     /locus_tag="VMU21_09100"
     CDS             8213..9208
                     /gene="hypE"
                     /locus_tag="VMU21_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HUO77719.1"
                     /translation="MDRIVLGHGSGGKLMHELIRDHFSPVFGIAGLGDSAILDMNPQK
                     LAFTTDSYVVSPLFFPGGNIGELAVYGTVNDLAVSGATPLFLTAGFIIEEGFPMEHLR
                     VIVASMAEAAGKANVKIVAGDTKVVNKGKGDGMFINTSGIGIFIGGIESSASRIRAGD
                     TILVSGQIGNHGVAVMSERNGITFDPPVKSDTAPLNSLIESMCEVTREVRFMRDPTRG
                     GLATTLKEVAMESGKCLRIKEKAIPVAPQVKGACDLLGLDPLYVANEGIVMAVVEPSM
                     AKAVLAVMRQHVYGVNAHIIGTVDESPRGMVLFETLAGGTRIIEMLQGEQLPRIC"
     gene            9239..9445
                     /locus_tag="VMU21_09105"
     CDS             9239..9445
                     /locus_tag="VMU21_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77720.1"
                     /translation="MKKKRARLMTAMRRAILPISTKRIPDNASNPAIGTKEALCKDFL
                     PWRKSNPTVKRIMMAIIPGNNFIP"
     gene            9582..10556
                     /locus_tag="VMU21_09110"
     CDS             9582..10556
                     /locus_tag="VMU21_09110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUO77721.1"
                     /translation="MISTKISGKFQTGVVKMFLSLVVLCLFPALSYPAALLDKVVAVV
                     NKEVITWGELYRTMEFETSNEMKALSDAEKHKIFKENEVAFLEKMIDMKLQLQLAKKL
                     DIDASKDEIAEAIEGIRKKYAMDDKEFQESLKKEGFTLEDYKKRLGEQIILSKVVNQQ
                     VKNKIVISEEEIKDYMAKNQGTEYRVRQIFFKKPDKDFDKGAMEAKAEEVLQKLKSGE
                     SFASLAQKYSDDPTAKTGGDLGFIRKELLSKEFLNVLSSMAVGSTSSPFWTEKGLHII
                     YLEEKVDAQNAAEFKELAMRKLLEKHFNEEYRIWIRGLRERAFVEVRL"
     gene            10556..11215
                     /gene="purN"
                     /locus_tag="VMU21_09115"
     CDS             10556..11215
                     /gene="purN"
                     /locus_tag="VMU21_09115"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HUO77722.1"
                     /translation="MPEKLALGVLASGRGSNFQAIVDEIEAGRLNASVKLLIVDDPKA
                     FAVERAKKHSIEYLCLQPREFSSKDAFFLKIVDELKMRGVKLVILAGFMRIVRRPLLD
                     AFPHQVMNIHPALLPSFPGLHGQKQAVDYGVKISGCTVHFVDEGMDTGPVIIQAAVPV
                     SPDDTEESLSARILKLEHRIFPEAIRLYAEGRLRIEGSVVRISGYGLDEGLIVNPPLR
                     G"
     gene            11328..11948
                     /gene="rsmD"
                     /locus_tag="VMU21_09120"
     CDS             11328..11948
                     /gene="rsmD"
                     /locus_tag="VMU21_09120"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HUO77723.1"
                     /translation="MIKISGGIAKGRRIGVKKAFAKKTGHDELRPTSSKVREALFDIL
                     RNELPGSSFLDLYAGTGGVGIEAMSRGAVEVVFVESNTIRAGMIRQILGRLHFMEHSK
                     VIEGNALEFVRREGMREHRYDIVYLDPPYHSEELTEMLPLLGEGRILKEGGVVVVEHF
                     SKTDMPEGIKWLTLRKRYKYGDTSLTAYTLKKGGPEQSTDYLGNKA"
     gene            11945..12442
                     /gene="coaD"
                     /locus_tag="VMU21_09125"
     CDS             11945..12442
                     /gene="coaD"
                     /locus_tag="VMU21_09125"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HUO77724.1"
                     /translation="MKRGIYPGTFDPITNGHLDLVRRALRIFEEIIVAVAPSPKKQPL
                     FTIEERLKLIREAVKHYRNVRVEAFNGLLVGYAKAKEGIAIIRGLRAVSDFEYELQMA
                     LMNRRLDMSVETVFLMPSEEYSFLTSTIVKEAASFGGSVTGLVPDAVGIALREKFRVI
                     ENFVE"
     gene            12455..>13450
                     /locus_tag="VMU21_09130"
     CDS             12455..>13450
                     /locus_tag="VMU21_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77725.1"
                     /translation="MPRTALPIPPYFNPEEVDRVWKVPYQQRAEEARSWAKKYGISPS
                     AADRFKVCVVAVDVQNTFCIPGFELYVAGRSGSGAVDDNRRLSEFIYRNLNAITQICP
                     TMDTHQVMQIFHPIYLVNERGEHPQPFTLISKEDVEKGVWKFNPALCHSFQIGEEYGQ
                     RHLLHYAKRLKEGGKYDLTIWPYHGMLGGIGHALVASVEEAIFFHSVARYSQPDFHVK
                     GDRPFTEHYSVLGPEVMKGPDGETIGQKSDKFFRKLLDFDAVVIAGQAKSHCVAWTID
                     DLLQDIIAKDRRLVEKVYLLEDCTSPVVVPGIMDYTEQADAAFRSFGEAGMHVVRS"
BASE COUNT         3547 a         2897 c         3768 g         3238 t
ORIGIN      
        1 accaaaccat aagcatcctc cctgataaaa ttggcctcgc cacgtgtcat cctcttcttc
       61 ctcgttatca taatcccgct cctcttgctc tccatctacc ccacagccgg ccctccttca
      121 aaaaactggg ggatggcaag ccgcatatgt gttgacaacg actaaaatcg aaagtagaat
      181 ttgacaagat tatctataga tgcatcaggg agagaggttt tcgtctatac ctgaaaaacg
      241 tgtggactta ctactcagtg gcagcaggtt caaataaatc gtgttccctt atgggaatgg
      301 aatggaggtg atgtgcaggc tattgtctag ggagaaaagg aaggcttatg tcatatgtat
      361 gggaaaggag ggtttatgaa gggttttaga attatgctgg ttttgttgat gattattgca
      421 ttgccggtgg cagctgcagc acaaaaggca aagaccattg atgaactggc aacgatgtat
      481 gacgcgtcaa gctgtaagtc ttgccatgca gaaatttatg ctgcgtggga gaaatcccac
      541 catgcgaggc cgttgatggg ggtaaagggc gggctgatgt tgacgcccct tgcactgaag
      601 aagggagaac caggcagcac tgcattttca gaagacagcc cgaagaaaat gactgtcaag
      661 aacttccctt gttttaaatg ccatctcccg caggccgtta tctctgcaga ggattcagtc
      721 gcagttgagt tagctgaagc gcttatcgca aaggattcgg ccaaggtcgc caaactgcag
      781 atcacctgta ttgtttgcca taataccaag gcgattatcc acaagaggga attgggtgag
      841 ccggaaaagg gagtcctgta cggcttgaaa gacgtggctt ctcacgggga caagacctat
      901 acaaaggtaa agaagagcgc gattatgaag gattcggtct tctgtggtca gtgccacggc
      961 ctcggtccga atctcgagtt cgaccaggcg ttccagtgcg ctaacctcta cggaagttat
     1021 ttgcacaact atgtgtcttc gggagggacg catacctgcc aggagtgcca catgccgaag
     1081 gtggatggca aggccgatca ccttattgcc ccgaatttta acgacgataa ggggacttcc
     1141 gaactgcttg caaaggcaat taatcttgat gtgcagacta tcggctatga atggctaaga
     1201 aaagcggcaa catacgtccc aaaaattgtg gttgagacga agatcactac cactgcaggc
     1261 cacagaatac ctgatggctg accctcccat aacagagtgg tcatggtagt gaccgcaaag
     1321 tcaatggatg gtaaggagat agggaaagtg gaaaagcact atcaccctca ggccacaaac
     1381 tgccgcgaca ccaaggagag atacggagca caatggaagg tcgcgaatct ccgtgataca
     1441 agcctgcagc ctaataagac caaggccgag acgattgagt ttgaacttcc ggcaggggtc
     1501 agggccgctg acgtgaccgt agatcttgtg tatgaggatg tcaacccgga taacaaatac
     1561 cccattcact cgataacgaa gagggtaagt cttgacaagt aaacatagac aaggttaagt
     1621 gttttggaga aagcccgccc ttttgggcgg gctttcttat ggtgtgaatc aagctggtga
     1681 ggaggtaagg tatccttcca caagtaaatc ttctcccagt cttttcacgg tagtattctt
     1741 taatctgtaa gcttcatcca agtctctcaa tgtcttgcct ccgactgccg gataagaagc
     1801 agtcccgccc ataatttttg gtgcgataaa gagcatgact ttatcaacaa tgccatcttc
     1861 aagggcatgg gcattgaggg aggacccacc ttctatcaca atagaagtaa ttcccttttc
     1921 tccaagcttt ttcataagcc aattaagttc aagtgtatgt ttatataata ttatataact
     1981 ccccatgcta aataacttat tagctttttt taggagtgcc ggattccttg tattttttgt
     2041 aacaataacg gtctctggag gcaccctgag tatctttgca tcaggagaaa cctccaagga
     2101 tgggtctatt aggattctca cgggattcct gccatttctg atccttgcag tgaactgggg
     2161 gtcatccgct tttaccgtac ctattgctgt cattaccgca tcaacgctgc ttctcaagcg
     2221 atgaaccatc attcgtgact tttccccggt tatccatttt gattgtcctg cccctgtggc
     2281 aatcttgccg tcaagggtca tggcgacttt gagtatcacg aagggtctcc ttgtggtgat
     2341 atattttatg tagtattcgt tcacttgttt tgccttttcc tccagtaccc ccgttaccac
     2401 ctctatcccg gcccgcttca actttttcat tccctttccc gaaacctttg gattcgggtc
     2461 tctcatcgcc atgacaaccc tcttaatccc cgaatcaatg atcgttcttg tacagggagg
     2521 ggtcctttta tcagtatgac agcatggctc aagacttacg tagagggttg atgctcttgt
     2581 tttttcacct gctgcggtga ttgccagggc ctccgcatga ggggttcctg gtttcctgtg
     2641 atatccttct gcaataattt tcccgttctt tacgataact gcacccacca tgggattcgg
     2701 actcgtcatt cctctggccc tggtggagag cataattgcc ctattcataa aaaaagcgtc
     2761 acgcatagtc taaaataaca taaaaatcct ttacatacca aaagaatcct aatacaatac
     2821 ctatgaaaaa aaggtgaaca tcccatctgt tcagtggtat gatataattt actgagacaa
     2881 ggagggaaga tgagcaatat acttttgggt cttatcaccc tttcatttat tgtggctgtt
     2941 gttgtggtta tttttgttat gattgagctg aggggggcaa tcagggcatt aaaagaattc
     3001 ataaagacca cagagaattc cctgaaaccg acccttgaag aattacagca gaccctcaag
     3061 agcctgagaa atgtgacgga caacgtaact ggggtcactg aagatgtgag agtactttcg
     3121 agttctgtca gggacgtggg cgagaatgtg aagcatgtga gcgagcttgt tgatgaggtc
     3181 acctcctctg cggtggtcag ggtctcgggc ctgagggcag gggttactgc agccatggag
     3241 gttcttttga agaatatctt cagtgggaga aagtaagtag agtgtgagaa gttcccgatt
     3301 aattggtttt ttgccgtgat tacggacgat taaatttgcc ttgaggcaac aggaaaggag
     3361 aagagaatat gaggcatgac gaagaatgcg gccatagtac gggttcccta tttcttgcat
     3421 tccttcttgg tggtctggca ggagcgggcg ctgccctgct tctggcgccc aaatcaggga
     3481 atgagacgag gcaacggatt agggagctgg cggaagaagt gaaggagaag acagaagact
     3541 atgttgagca ggcgaaggga agggtcagtt caaccgtcga aaaggggaaa ggctatattc
     3601 aggaaaaaaa gtccattgta accactgcgg ttgaggctgg gaaggaagcg tacgaaaagg
     3661 agaaggaaag gctggcaaaa gagagttaat gcatgagact tccattgcgc tcggcatcct
     3721 ggacattata agcgatcggt gcattgctga agggtatcat tctatcgagt ctgtgaggct
     3781 caggatcgga agggccgcag gcatcatgcc tgaggccctt ctttttagct tcgacgttgc
     3841 aaaagagggt acccttgcgg aaagcgcgaa gctcatcatc gagagtgtct cgcttggggg
     3901 agtatgcagt gaatgcaatg ccgagtttga ggttgaagag gcgtatgtgc tcagttgccc
     3961 tgaatgcggt tccgcggcct tcaagataca gagaggctat gaaatggaaa tcgtcgagat
     4021 ggacgtgaag gaagagaaac acaatatatc gccatgattg atggaggtaa ttgatgaagg
     4081 tgaaagtagt aacacggatt cttgaggcca atgagaggct ggcagaggag aacagaaaac
     4141 ggtttaagga agcgggcgtg tttgttatga accttatggg tgcgccgggt gcaggcaaga
     4201 cgtctctcct cgaaaagacc attctggaac tgaagaccag ggttcgcatc ggggtgatcg
     4261 aaggagatat tgcgggaacc gatgatgcgg aacggattca taatacaggg gtcccggtgg
     4321 tccagatcaa taccggtggt gcatgccatc tggacgcgaa catgataagc gaggtcctgc
     4381 ctgagatgcc gttgggggaa ctggatatgg taatagtaga gaatgtggga aatcttgtct
     4441 gccctgcgga attcaaggtt ggtgaggata ttaaggcaat ggtcttgagt atcactgagg
     4501 gacacgacaa gcctttgaaa taccccctga tgtttcagga gtcagcagcc cttgtgctca
     4561 ataaagtgga ccttctccct tatgtaaatg tggacatgca aaaactgaaa aacgacgcca
     4621 tctcccttaa ccccaaactg aagatttttg aagtttcgtg caagactggt gaggggcttc
     4681 atagatggac tgaatggttg aacgggctaa aataacccta aagggcgttg ttcagggtgt
     4741 ggggttcaga ccgtacgtgt atatcctcgc tgaatccctt cagctcaggg ggtatgtgac
     4801 caacaccccc gatggcgtga ccatagatat agagggggag cacgtctctg aattcataga
     4861 gagacttccg aaggatcccc cgcccctttc tcagatagcc gctatctcca taacccctct
     4921 ccctgaatat ggatatggcg acttcaggat aatcgaaagc agggaggaga gcagccgggt
     4981 ccccttcacc ctaatctcac cagacgtgtc catctgtgat gactgcctgg aggaattgat
     5041 ggatccccat gacaggcgat atctctatcc ctttatcaac tgtaccaact gcggcccgcg
     5101 atatacgatc ataaggtcca ttccctatga cagaccccat accactatgg cgaatttttc
     5161 catgtgtgcc cagtgcaatg ctgagtatca cgacccccat gacaggcgat tccatgcaca
     5221 gcccaatgcg tgtccacaat gcggccccaa tgtgacactc gtgaacagcc ggcaacctga
     5281 actggatggc caagtggcca tggaaaagac aatagaattg ctgaaagcag ggaggattat
     5341 tgccattaaa gggcttggtg ggtttcacct tgcatgcgat gcgacgaaca aaactgcagt
     5401 ggaggagttg agggaacgaa aaagaaggag taataagccg ttcgcattaa tggcccctga
     5461 cttattagca gtggagcgtt tctgcacagt ttcagaagaa gaggcaaaac tcctgtcatc
     5521 ccggcggcgc cccattgtac tcctcaggaa aaaggaggcc gtatccctgc tgcccgaggc
     5581 tgtctctccc cataaccaat atttggggtt catgctgcct tacacacccc tccattatct
     5641 gttgtttcac cagccatttc ctgccagaga aggtgggatg ctcagcggga gggaagccaa
     5701 tttttccgcc cttgtgatga ccagcggcaa tctgtctgag gagccgattg tgagggataa
     5761 tgaggaagcg cttgagaagc ttcaaggtat cgttgacgca tttcttctcc acaacaggga
     5821 cattttcatg agggtggatg attcggtgat acgggtgaga tatgaggggc ctacagtacc
     5881 ttgtcaggaa ggcagtatcc tgtcagataa gcgccgggag tccacccttt tcctgcgccg
     5941 ctcaaggggt tatgcgcctg aaccattggc actccagagt aatggccctg aggtcatggg
     6001 gtgcggcgct gacatgaaga atacgttcac cgtcaccaaa ggggggtatg ccattccaag
     6061 ccagcacatt ggggacatgg agaattatga aactgtgcaa ttttatgagg aatgcctcga
     6121 aaacttaaag cgtgtctgca gggtaaagcc agttgccatg gcgcatgacc ttcatcctgg
     6181 atacctctcc acacaatggg ccttaaggca gggagttcag acttatggaa tacagcatca
     6241 ctatgcacac atcggttctg ttatggcaga gaagggtctg cttggcaaag ttcttggtgt
     6301 agcctttgat ggtacaggat atggtacaga cggaaatctc tggggagggg aggttcttct
     6361 tgcagatgtc gatggatttg agagggtggc ccatttcaaa tatatttccc tcccgggagg
     6421 tgagagggca gtgaaggagc cgtggagaac agccataagc tatatccttg atgctgccgg
     6481 agatgaggcg atggagtatc tggagaaaat aggtttctta gagaaattcg ggaaggagcg
     6541 aacagaaaag atcatcaaga tcgcgggtgc gagggaactt tcccctcagt catccggtgc
     6601 cggaaggctc tttgatgcgg ttgcggcgct tacaggggtg tgcgatataa ataccttcga
     6661 aggggaggcg gcaatggcat tggaatccct tgtctcagga ggtactgagg aggactatcc
     6721 ggtgaatata attttggaga agtctatgac catagacttt tcacacacca ttatcggtat
     6781 cgtcaatgat atagtgaatg gtacggagaa gcacctcatc tcgacaaaat ttcataacac
     6841 ggtctctaat gttatactaa aaacagtggg cagactttgg gagaccttca tgctcgaaga
     6901 ggtggtttta accggaggca cgttccagaa tctctacctc ctggagagaa caacgaggat
     6961 gctggcagca acgggaatga aggtatttat taatgaaatg gttccctgca atgacgcgtg
     7021 tatatcctta ggacaagcct atctcgtcag ggaaagaata aagaataagg gttagggagc
     7081 tttgaagaaa cctaaaagca aaagcgtcca caagattctg tatctctgtt aagaaatcag
     7141 aaccgggggc tgggaatgaa gggaatgctc aatgacataa aaagaatgat ggacctaatg
     7201 ggaaggccgc tcaagctcat ggaagtgtgc ggcacccaca cggttgccat cttcaggcac
     7261 ggtatcaggg atatcatccc cagagaaatc agtctgttga gcggacccgg ctgcccggta
     7321 tgcgtgactt ccattaagga tgtggattct gccatcgccg ttgcgaaact tccgggtgtt
     7381 attctcggaa cattcggaga catgatgcgt gttcccgggg gagtggaatg ccttgccgat
     7441 gcgagggccg aaggtgcgga cgtgagggtt ttctattcgc ctatggatgc gttaaaactt
     7501 gcgatggatg aaaaggacag acaggtggtg ttctttgcca cagggtttga gacgacgtca
     7561 cctctcatcg ctgctacgct ttttgatgct gcgaaacggg gcgtcacaaa ttttttcatt
     7621 tattccaccc ataagcttgt cccccctgcc ctgagggcct tgctcggttc tcctgatgtg
     7681 atggtggatg gatttatcct cccgggacat gtgagcacca taatcggcaa aaaaccttac
     7741 gagttcctgg cgtccgaata taagaagcct ggggtcatca caggcttcga cgcagatgat
     7801 atcattgaag gcatacatat gcttgtcagc caaatgctcc acggtgatgc tgaagtggag
     7861 atacaatata aggatgtagt aagagaagag ggcaatccaa gggcagtttc attaatagag
     7921 gaggtgttta caccagcgga cgcttactgg aggggaatag gaacaatacc gcaaagcggg
     7981 ctcacattga gggaggaatt caaaagattt gacgccatcg gaaggttcag tcccgaagtg
     8041 ccctatcatc ccgagcccga ggcatgttcg tgcggtgatg tgctcagggg tgtaaagata
     8101 cccctggaat gtcctctgtt cggcaccgca tgcagtcctg aaagccctgt gggggcatgc
     8161 atggtgagca ccgaggggag ttgcgctgcg tactataaat atggagcata ccgtggatag
     8221 gatagtcctg ggtcatggaa gcggaggaaa actgatgcac gaactgatca gggatcattt
     8281 ttctcctgtg ttcggcattg ccgggcttgg tgattcggcg atccttgaca tgaacccgca
     8341 gaaacttgcc ttcaccaccg attcatatgt tgtttccccg ctcttcttcc ctggtggcaa
     8401 tatcggtgag cttgcggttt atgggacggt gaatgacctt gcggtctctg gcgccacacc
     8461 cctctttctc accgcagggt ttatcataga agaaggattt ccgatggagc atctgagggt
     8521 tatcgtcgct tcaatggccg aggcagcggg gaaggcgaac gtaaagattg ttgcagggga
     8581 tacaaaggtg gtgaacaaag ggaagggcga cgggatgttc ataaacactt ccggcatcgg
     8641 aatcttcatt ggaggcatag aatcctctgc ctccaggatc agagcgggcg acacaattct
     8701 ggtgagtggt cagatcggca accatggggt tgcggtgatg tcagagagaa acggaatcac
     8761 ctttgatcct cccgtgaaga gcgataccgc gccgctcaac tcactgatag aatccatgtg
     8821 tgaagtgacc agagaggtgc gctttatgag ggatcccacg aggggagggc ttgcaacaac
     8881 cctgaaggag gttgccatgg aatcggggaa atgcttaagg atcaaggaaa aggctattcc
     8941 cgttgctccc caggttaagg gcgcctgtga ccttctgggt cttgatcccc tctatgttgc
     9001 caatgagggg atagtgatgg ctgtggtgga gccttcaatg gcgaaggcgg tgcttgcggt
     9061 gatgaggcag catgtgtatg gagtcaatgc ccatattatc gggactgttg atgaatcacc
     9121 gaggggtatg gttctttttg agaccctcgc ggggggtacc cggatcatag agatgcttca
     9181 gggcgagcag ttgccgcgga tatgttgagc catacgcaag ggtcaggctc tttttgagat
     9241 gaaaaaaaag agggcaaggc tgatgactgc gatgagaagg gcaattttgc ccataagcac
     9301 aaaaagaatt cctgacaatg cgagcaaccc cgccattggt acgaaagagg ctttgtgcaa
     9361 agattttctt ccctggagaa agagtaatcc tacggtgaag agaattatga tggcgattat
     9421 ccctggcaac aacttcattc cctagtctaa aaggtgtgtc gaaaaatgac aagcccgcag
     9481 tgagccatgg ggaatataat tattggttga aattttgtag tcacttaata taggtttaaa
     9541 gcgtaaatga acagcacaga gcaaaaggat aggggaacgg aatgatatcg acaaagattt
     9601 ctgggaaatt ccaaacgggt gtagtgaaga tgtttttgag cctggtggtc ttatgcttgt
     9661 tccctgctct ctcttatcct gcagcccttc tggacaaggt tgtggcggtg gtgaacaaag
     9721 aggtgatcac ctggggtgaa ctatacagga ccatggagtt tgagacgagc aatgaaatga
     9781 aggctttgag tgatgcagaa aagcataaga tatttaagga aaatgaggtg gcttttctcg
     9841 aaaaaatgat agacatgaaa ctccagttac agctcgcaaa gaagctggac attgatgcga
     9901 gcaaggatga gattgcggaa gcgattgagg gaataagaaa aaagtacgca atggacgata
     9961 aggagttcca ggaatccctg aagaaggaag ggtttaccct ggaggattac aagaagcgtc
    10021 tgggggagca gattatcctc agtaaggtgg ttaaccagca ggtgaagaac aagattgtga
    10081 tatctgaaga agagataaag gactacatgg caaagaacca gggtacggag tacagggtgc
    10141 ggcagatctt cttcaagaag ccggacaagg attttgataa aggggcaatg gaggcaaagg
    10201 cagaagaagt gttacagaaa ctgaagagcg gggagtcttt tgcttcactt gcccagaagt
    10261 actctgatga cccgacggcg aaaacaggag gggacctggg ttttatcagg aaagagcttc
    10321 tcagtaagga gttcctcaac gtgctctcct ccatggcagt cggcagtacg agtagcccgt
    10381 tctggacaga gaagggcctt catatcatct atcttgaaga gaaggttgat gcgcagaacg
    10441 ccgctgagtt caaggaactt gccatgagga agcttttgga aaagcatttc aatgaagagt
    10501 atagaatctg gatcaggggc ctgagggaaa gggcatttgt ggaggtgaga ctctaatgcc
    10561 ggagaaattg gcgctcgggg tgcttgcatc gggcaggggc tcaaattttc aggcgattgt
    10621 tgatgagatt gaggccggaa ggcttaatgc ctcagtaaag ctcctcattg ttgatgaccc
    10681 caaggccttt gccgttgaaa gggcgaagaa gcactcaatt gaatacctct gccttcagcc
    10741 cagggagttt tcctcaaaag acgcattttt cttaaagatc gtagatgagc ttaagatgag
    10801 gggcgtaaaa ttggtgatcc tcgcgggatt catgcgtatc gtgagaaggc cccttctcga
    10861 tgcctttccc caccaggtaa tgaatatcca cccagccctt ctcccctctt tccccgggct
    10921 tcacggccaa aagcaggcgg ttgattatgg agtgaagatc agcgggtgta ccgtgcattt
    10981 tgtggatgag ggtatggata ccggaccggt gatcatccag gcagcggtgc cagtgtcacc
    11041 ggacgacaca gaggagtccc tctccgcgag gatcctgaag ctggaacaca ggatatttcc
    11101 tgaggccata aggctctatg cggaggggcg actcaggatt gaggggagcg tagtaaggat
    11161 atcaggctac ggattggatg agggacttat cgtgaacccc ccattaaggg gttagtgagg
    11221 caatgtctga ccctgaactc cttttgtttc caaaaaacag ggaccttgag tccctcgcac
    11281 acattgagat aaccataatg gtcatctgag gatctgaagg gctgcttatg ataaagatat
    11341 ccggtggcat tgcgaaagga agaagaatag gggttaagaa ggccttcgcg aaaaagaccg
    11401 ggcatgatga actgagaccc acatcctcaa aggtgaggga agcccttttt gacattctga
    11461 ggaatgaatt gcccggatcc tcattcctgg acctttatgc agggacgggg ggtgtgggaa
    11521 ttgaggcgat gagcagggga gccgtagaag tggtctttgt tgagtcaaac acgatccgtg
    11581 ctggtatgat acgccagatt ctcggccggc ttcattttat ggagcattcg aaggtgatag
    11641 agggaaacgc ccttgagttt gtgcggagag aagggatgag ggaacaccga tacgacattg
    11701 tgtatctcga tcccccctat cattcagagg aactcactga gatgcttcct ctcctgggag
    11761 aggggaggat tctcaaggaa ggcggtgtgg ttgttgtcga gcatttttct aaaacggata
    11821 tgcctgaagg catcaaatgg cttactctga ggaaacgcta caaatatggt gatacaagcc
    11881 tcacggcata cacactgaag aaagggggcc cagaacaaag cacagactat ctgggaaaca
    11941 aggcatgaag agaggcatct atcccgggac atttgatccc ataaccaacg gacacctcga
    12001 tcttgtgcgg agggcattga ggatattcga ggagattatt gttgcagtgg ctcccagccc
    12061 gaaaaaacag ccgctcttta caattgagga gaggctgaaa ttaataaggg aggccgtgaa
    12121 gcattatcgg aatgtgaggg tagaggcgtt taatggattg cttgtgggtt atgcgaaggc
    12181 aaaggagggt attgccatca tcaggggatt aagggcagta tccgattttg agtatgagct
    12241 ccagatggcc cttatgaacc ggagactcga tatgagtgta gagacggttt tccttatgcc
    12301 gtcagaggag tactcgttcc ttacttctac tattgtcaag gaggcagcct cgtttggggg
    12361 atcggtcacg ggtcttgtcc cggatgcggt gggaatcgcc ctaagggaaa aattcagggt
    12421 catagagaat tttgtggagt agggaggata tcgtatgccg cgcaccgcgc ttcccattcc
    12481 cccttatttc aatccggaag aggttgacag ggtctggaaa gtcccctatc agcagcgggc
    12541 tgaagaggca aggagttggg caaagaaata cggaatatcc ccctctgctg ctgatcgatt
    12601 taaggtctgt gtagtggcgg tggatgtaca gaataccttc tgcatccccg gatttgaatt
    12661 atatgtggcc ggccgttcag gcagcggcgc tgttgacgac aaccggaggc tttcggaatt
    12721 catttaccgt aacctcaatg cgattaccca gatctgccct accatggata cgcaccaggt
    12781 aatgcagatt ttccatccga tctaccttgt caacgagaga ggggaacatc ctcaaccgtt
    12841 cacgctgatt tcgaaggaag atgtggagaa aggcgtgtgg aagttcaatc ctgcactttg
    12901 tcacagtttt cagatcggcg aagagtacgg acagcgccat cttcttcact atgcgaagcg
    12961 actgaaggag ggagggaagt atgatctcac catatggcct tatcacggca tgttgggggg
    13021 aatcgggcat gcccttgttg cctctgtgga ggaagccatt ttttttcatt ccgtggcccg
    13081 ttacagtcag ccggatttcc acgtgaaggg agacaggcct tttaccgaac actactctgt
    13141 tttgggtccc gaggtgatga aaggtccgga tggagagact atcggccaga agagcgacaa
    13201 gttcttcagg aaactcctgg attttgatgc ggtggtcatc gcgggacagg ccaaaagcca
    13261 ctgcgtggca tggacgatag acgaccttct tcaggacatc atcgccaagg acagacgatt
    13321 ggtcgagaag gtatacctcc ttgaggactg cacttcaccc gtggtggtac cggggataat
    13381 ggattacacc gagcaggctg acgctgcgtt caggagtttc ggggaagcgg ggatgcatgt
    13441 ggtcaggtct
//
LOCUS       DATKOU010000197         6708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4895531, whole genome shotgun sequence.
ACCESSION   DATKOU010000197 DATKOU010000000
VERSION     DATKOU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4895531"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VMU21_09135"
     CDS             complement(<1..501)
                     /locus_tag="VMU21_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUO77726.1"
                     /translation="MTLRNARFKCLFFTEIFLLALLFASPLYSQDYIVGERDVLKITV
                     YDNPDLTTVARVSEEGSIFFPFLGEVKVKGLTTPQIAAKISSMLAEGYIVDPQVNVYI
                     EQFRSLKVMLIGEIARPGLYELPVATKLLELLLRAGPVTPNAGDEAIIKRKSPQGEQS
                     ITVDLGK"
     gene            complement(509..1678)
                     /locus_tag="VMU21_09140"
     CDS             complement(509..1678)
                     /locus_tag="VMU21_09140"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUO77727.1"
                     /translation="MKKYIFGTAFFLLGLLIASDSYAYRIGFFEFRPSLAVTEVYSDN
                     IFCTNTDTVSGFRTIFSPGFNIELPMVAVTRDRSRYHFDMQFRLDAEELNKNTFSNGV
                     GYITMAKFGYKMPSTGITLDLYELFHRAHDPYSVNVTPQLDWYHENAVTASLGYEFAS
                     RYKIRFDFTNHILNYDADRVSYRNDTDNTIAGYFYYAFTPKLSAFVEYQGVKFDYNDH
                     ILNNTENYLYGGIEWDVTAKTKGTIKAGYESKKFDDPSLGKYNNFAFSADINHNFDPK
                     NSVMLTAYRRPNSTNLVGTNFYLTTGFLLQYYYRITGKITARGDLSYGNDKYYGLLGG
                     NPRTDNTWSGAIWLFYQIKRWLRVELGYMYTNRNSNVDVYTYKSNQIWLTLKATP"
     gene            complement(1888..2373)
                     /locus_tag="VMU21_09145"
     CDS             complement(1888..2373)
                     /locus_tag="VMU21_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77728.1"
                     /translation="MTKVLITVCIVAILISLIPSPAISQVKDIPPPDTSIRYTLAKEL
                     STGKGVCAVVVDLIKAGMSSAQVVENAILLGHSPCVVVRCAIDAGGELEDVITAAFRA
                     DANSEVILTCCILAGIPIDALARILECRGYPCLWDNVITARAATVGGGGGGIPVSPFR
                     P"
     gene            2439..3239
                     /gene="panB"
                     /locus_tag="VMU21_09150"
     CDS             2439..3239
                     /gene="panB"
                     /locus_tag="VMU21_09150"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HUO77729.1"
                     /translation="MANITVNDFLRKKAERKKISMITAYDYPFALIADEAGMDAILVG
                     DSVGMVVQGLENTLPVTMDEMIYHVKMVSRAVKNAMVIGDMPFMSYHGSREEAVKNAG
                     RFLKEAGASAIKLEGGSEVEGLIRAMTRADIPVMAHIGLTPQAIHRMGGFKVQGRSEE
                     AARRLLEESHLVEDAGAYALLLEAIPMDLAKKVTEELSIPTIGIGAGPYCDGQVLVLH
                     DVLGLFERFVPKFVKRYADLRKIALEAMTAYRDEVEKGLFPLDEHSFK"
     gene            3298..3825
                     /gene="scpB"
                     /locus_tag="VMU21_09155"
     CDS             3298..3825
                     /gene="scpB"
                     /locus_tag="VMU21_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HUO77730.1"
                     /translation="MEDRENKSLLEALLFLSGEPLTTSGLKTITDLDEPGIQRLMNEL
                     VAEYRTRDGGVQIAEIANGYQMVTNSRYASWVKKLVGSESTRLSLAALETLAIIAYKQ
                     PIIKAEVEQVRGVNSDGVIKTLLERRLIKIMGKKEAPGKPLLYGTTKDFLQYFGLKDL
                     AELPTLKDLAREEAL"
     gene            3854..4993
                     /locus_tag="VMU21_09160"
     CDS             3854..4993
                     /locus_tag="VMU21_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUO77731.1"
                     /translation="MRVTPFWIACTCFLLLHAMSAGAEQTYTVKKGDTLYKISKKFKV
                     DLQEIQETNGLDSAKLRPGSTLAIPSKRGNEKSHRGRPSVQETIKPREKFVANTTILA
                     TADTPPSPYETQYHKVKKGETLASVSRKYSLTLNELKGLNNIEKSIRLKPGQKLIVKQ
                     TGPKTYSVRKGDTLVKISKRFHTSPEELMEINELDSGELRPGQKLLLEVWLDPEETKP
                     SPVIADAIISDDLHAMTESTDLNSLSVRDRLILFAKKMLNIPYRFGGSTFMGIDCSGY
                     VQKVFGLLDVSLPRTAREQFHMGESVSKEELSMGDLVFFRTYASFPSHVGIYLGNNLF
                     IHASSKNRKVSIDSLDAPYYFKRFIGAKRVFKDEVSETTAPLEKG"
     gene            complement(5128..5865)
                     /locus_tag="VMU21_09165"
     CDS             complement(5128..5865)
                     /locus_tag="VMU21_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO77732.1"
                     /translation="MIRKSILLVEDEAVVREMIRGALEREYNVLEASKYSEAIDHLKD
                     RIDLALIDYALPDRDGFEVLRAIREVKPALPAIMMTAYSSENIAIKALRVGATDYIKK
                     PLSFAYLMKRVSQILGGDLQAKMNGDAQTREEFIMEGIAAYIEDKYREELSLDQLAAM
                     VCMNRFKFCRAFKKKFGQSFVSYLSGTRVKKAAKLLREHPDLNITEIALAVGYGNVGH
                     FGRVFRTVHGVSPTEYRRCHGGGGDRK"
     gene            complement(6007..>6708)
                     /locus_tag="VMU21_09170"
     CDS             complement(6007..>6708)
                     /locus_tag="VMU21_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUO77733.1"
                     /translation="IVCIGSHDNTLDILSNILKKRHPQFSFSSAHVGSMGGLIALKKG
                     EAHIAGTHLLDEETGLYNVPFIRRLLPERKIVLINLTYREQGFLVKKGNPKGIRGFED
                     LTRDGVVFINRQSGAGTRLLTDKHLRESGIEPSRIVGYEREEYTHMGVASAVLSGIAD
                     TGLAILAAANALDLDFVPVAKERYDLAIPTDFMELEMMRTLLDIIRNDEEFRKTVIAM
                     GGYDLSDMGKVMYGG"
BASE COUNT         1802 a         1646 c         1533 g         1727 t
ORIGIN      
        1 tttaccgagg tcgacggtga tgctctgctc accctgcgga gactttctct ttatgatggc
       61 ctcgtctccc gcattcgggg tgaccggtcc tgccctcaag agcagttcaa gtagctttgt
      121 ggctaccggc agctcataaa gtcctggcct tgcaatctca ccgatcagca tcaccttcaa
      181 gcttctgaat tgctctatat agacattcac ttgaggatcc acgatatacc cttcggcgag
      241 catcgaactt atcttcgctg caatctgggg tgtggtcagg cccttcacct tcacctcacc
      301 caggaaaggg aaaaaaatgc tgccctcttc gctcaccctt gccacagtgg tcaggtcggg
      361 gttatcgtac accgtgatct tcaacacatc cctttcgccg acgatataat cctgggaata
      421 cagcggagat gcaaaaagca gagcaaggag gaaaatctct gtaaaaaaaa gacatttgaa
      481 ccgcgcattc ctcaaagtca cgccctcgct acggtgttgc cttgagtgtg agccatatct
      541 gattactctt gtaagtgtat acatcgacat tcgaatttct attagtatac atatatccga
      601 gctcaactct cagccagcgt ttgatctggt agaagagcca gatggctcca ctccacgtgt
      661 tatccgtcct cggatttcca ccaaggagac cataatattt atcatttccg taacttaaat
      721 ctccccgtgc agtgatcttc ccggttatcc tgtaatagta ctggagcagg aaacctgtag
      781 tcaaataaaa attggtacca accaagttcg tagaatttgg tctcctgtac gcagtgagca
      841 taacagaatt cttgggatca aaattgtggt ttatgtcggc gctaaatgca aagttgttgt
      901 atttgccaag ggacgggtcg tcaaattttt ttgactcata tcctgccttg atcgtcccct
      961 tggtctttgc ggtcacatcc cattcaatgc ctccgtaaag ataattttcc gtgttgttca
     1021 aaatgtggtc gttgtaatcg aatttcacac cttggtactc cacaaatgca gagagcttag
     1081 gagtaaaagc gtaataaaaa tacccggcaa tggtattgtc cgtgtcgttc ctgtagctga
     1141 ctctgtcggc atcataattg aggatatgat tggtaaagtc gaatcttatc ttatagcgac
     1201 tggcgaactc gtatcccagg cttgccgtta cagcattttc atggtaccaa tcaagctgag
     1261 gagtaacatt tacgctatac gggtcatggg ctctatggaa aagttcatac agatccagtg
     1321 taataccagt agaaggcatt ttgtatccga actttgccat tgtgatgtaa cctaccccat
     1381 tgctgaacgt gtttttattg agttcctcag catccagcct gaattgcata tcaaagtggt
     1441 atctgctcct gtcccttgtc accgcaacca taggcagctc tatgttgaac ccagggctga
     1501 agatggtcct gaaaccactg accgtatcgg tattggtaca aaaaatatta tcgctgtaca
     1561 cttctgtcac tgccagagag ggacgaaact cgaagaaacc gattctgtat gcataagaat
     1621 cgctggctat aaggagacca agcaagaaaa atgcagttcc aaatatatat tttttcattc
     1681 caataacctg taagcaagta aaatgccaga tgcatttttt acagaaactt gtttacaaaa
     1741 cacaaaattg tcatagttta caatgaatta gaaaactttt gcaacaacaa aatgaagaga
     1801 aagggaggca gggctgttat ttcacagtaa atcttcgatc aactgtctta ttttcataca
     1861 tagggcaaaa ggacgggtgt ggcttattta cggcctgaag gggcttaccg gaatgccccc
     1921 gccaccaccg cctacagtag cagcacgagc tgttattaca ttgtcccaga ggcaagggta
     1981 accccggcat tccagtatcc tcgccagggc atcaatcggt attccggcca gaatacagca
     2041 ggtgagaatc acctcggagt tcgcgtctgc cctgaatgcc gcggttatca catcctccag
     2101 ttctccccct gcgtctatgg cgcaccttac taccacgcac ggggagtggc ccagcagtat
     2161 agcattttcg actacctggg cggaactcat tcctgctttt atcagatcaa ctaccaccgc
     2221 gcatacacct tttcctgtgg agagttcctt tgcgagagtg tatctgatgg aggtatcggg
     2281 agggggaatg tctttgacct gcgagatcgc aggagaagga ataaggctaa taagaattgc
     2341 cactatacac actgtaatta agacttttgt catcctcata aagtaagaat atcatggcat
     2401 tgacggtttg tcaagaacat tgatacaata gcttcgccat ggccaacatc acggtaaatg
     2461 attttctcag gaaaaaagct gagaggaaaa aaatcagtat gattactgcc tacgactacc
     2521 cctttgccct aattgccgat gaggcaggga tggacgcaat tctcgtgggg gattctgtgg
     2581 gcatggttgt tcagggtctt gaaaataccc tccctgttac catggacgag atgatttatc
     2641 atgtaaagat ggtatcaagg gccgtaaaga atgcaatggt aataggggac atgcccttca
     2701 tgtcctatca cggaagcagg gaagaagcgg tgaaaaacgc ggggagattt cttaaagagg
     2761 cgggtgcgtc tgccataaag cttgagggag gatctgaggt tgagggactt atcagggcca
     2821 tgacaagggc ggatatccct gtcatggcac atatcggact cactccccag gcgatccacc
     2881 gaatgggcgg gtttaaggtc cagggacggt cagaagaggc ggcgagaaga ttgcttgagg
     2941 aatcgcacct ggttgaggat gcaggtgcat atgccctttt gcttgaggca attcccatgg
     3001 atcttgcaaa gaaggttact gaagaacttt caatccctac aataggaatt ggtgcaggcc
     3061 cttattgtga cggacaggtt ctggttctcc atgacgtact gggattgttt gaaagattcg
     3121 taccaaagtt tgtcaagcgc tatgcagacc tcagaaaaat cgcgttggag gccatgactg
     3181 cttataggga cgaggtggaa aaagggcttt tccctctgga cgaacacagc ttcaagtaat
     3241 agattaaata ataattgaaa tctcttaaca tttctgctat attgatacaa gttttgtatg
     3301 gaagataggg aaaataaatc gctgttggag gcactattgt ttctttccgg cgagcctttg
     3361 actacgtcag gcctaaagac cattaccgac ctggacgagc ccgggattca acggctcatg
     3421 aatgagcttg ttgctgagta ccgcacgagg gacggaggcg tccaaattgc ggagatagcc
     3481 aacggctatc agatggtcac caactctcgt tatgcttcat gggtgaaaaa gctcgtgggc
     3541 tcggagtcta cgagattgtc ccttgcggcg cttgagacgc tcgcgattat cgcctataag
     3601 cagcccataa tcaaggcaga ggtggaacag gtcagaggcg taaattctga cggcgtaata
     3661 aagaccctcc tcgagaggag attaataaaa ataatgggca agaaggaggc gcccggcaaa
     3721 ccgcttctgt acggaaccac aaaggatttt ttgcagtact tcggattaaa agaccttgca
     3781 gagttgccaa ccctaaagga ccttgcaagg gaggaggcac tatagaccgc gacacacaga
     3841 gaggaggggc agcgtgaggg taactccttt ttggattgcc tgtacatgtt ttttgctact
     3901 ccatgcaatg agtgcaggtg cggagcagac ctatactgtc aaaaagggcg acactcttta
     3961 caaaatctcg aaaaaattca aggtcgacct gcaggagatt caggaaacca atggcctcga
     4021 ttctgccaaa ctcaggcctg gttcaacctt agcaataccc tccaagagag ggaacgaaaa
     4081 aagccacagg ggaaggccct ctgtgcagga aaccattaaa ccacgtgaaa aattcgtggc
     4141 gaataccaca atcctggcga ctgcggacac acccccctcg ccatatgaaa cccagtacca
     4201 taaggtcaag aagggagaga ccctcgcctc tgtctccagg aagtattcgc tcacattaaa
     4261 tgaactgaag ggattaaaca acattgaaaa atccatcaga ctgaaacccg gccagaaact
     4321 catcgttaaa cagacaggcc cgaagaccta ctcagtaaga aaaggcgaca ccctggtgaa
     4381 gatctcaaaa agatttcata cgtccccaga ggaactgatg gagattaatg aactggattc
     4441 aggtgagtta agacccggac aaaagctcct cctggaggtt tggcttgacc cggaagagac
     4501 aaaaccctcc ccggtaatag ccgacgcgat catttccgat gaccttcatg ctatgaccga
     4561 atccacagat ctcaattcct tgagcgtaag agaccgcttg atactctttg ccaaaaagat
     4621 gctgaatata ccctaccgtt ttggaggaag tacctttatg ggaatcgact gttccggata
     4681 tgtccagaaa gtgttcggcc ttttagacgt atcacttcca aggaccgcac gcgaacaatt
     4741 ccatatgggt gagtcagtaa gcaaggagga actttccatg ggtgatcttg tgttcttcag
     4801 gacctatgcc tcattccctt cccacgtggg gatctatctt ggaaacaacc ttttcataca
     4861 cgcctcttca aaaaacagaa aggtctccat cgacagtctc gatgccccgt attatttcaa
     4921 gcgcttcatc ggcgcaaaga gggtcttcaa ggatgaggtg agcgaaacga ctgccccgct
     4981 tgaaaaaggc tgattcatta ttgccggaag attctgggcg tgatcaaaac cacgcaaagt
     5041 atctctcccg ttccccaaag cgtatatcat gagtatcctt ctcgcacttt gcaaggcggt
     5101 ttcttcgttc tgcccttagc tccagggtta ttttcggtct cctccgcccc cgtggcaccg
     5161 gcgatattca gtgggtgata cgccgtgaac tgttctgaat accctgccga aatgtcccac
     5221 attcccatac cccactgcaa gggcaatctc ggtgatgtta aggtctggat gctctctaag
     5281 gagtttggcc gccttcttaa cccttgtacc actgagatat gaaacaaagc tttgcccgaa
     5341 cttcttctta aatgccctgc agaacttaaa cctgttcata cataccatgg ctgcgagctg
     5401 atcaaggctc agttcctccc tgtatttgtc ttcaatatag gcggcgatgc cttccataat
     5461 aaattcttct cgagtttgtg catcgccgtt catttttgcc tgcagatccc cgccaaggat
     5521 ctgcgatacg cgtttcataa gatatgcaaa gctcaaaggc ttcttgatgt agtccgttgc
     5581 gccaaccctc agcgccttga tcgcgatatt ttcgctgctg tatgcagtca tcataatcgc
     5641 ggggagcgcc ggtttaacct cgcgtatcgc ccttaatact tcgaacccgt cgcggtcagg
     5701 aagggcatag tcaataaggg caagatcgat cctgtctttg agatggtcaa tggcctcgga
     5761 gtactttgat gcctccagca cattatactc ccgctcaagc gcaccccgta tcatctccct
     5821 taccactgct tcgtcttcca ccagaaggat ggatttgcgt atcatatctc ctctttcaac
     5881 ccataggatc actagtggta tctgactttc tcatatcaag aatcaagtct tttttcaaat
     5941 ggtatgaagt gtcctttccc tctctcatcg tactaaaaaa agaaacctaa aatgaagcct
     6001 tcagcttcat cctccgtaca tcacttttcc catatcagag agatcgtatc ctcccatagc
     6061 tatgactgtc tttctaaatt cttcatcatt cctgatgata tccaaaagtg tcctcatcat
     6121 ctccaattcc ataaaatccg tgggtatggc aagatcatag cgctcctttg ccacaggcac
     6181 aaaatccagg tccagcgcat ttgctgcggc cagaatggcg agccctgtat cagcaatgcc
     6241 gctcagtacg gccgaggcaa cacccatatg ggtatactcc tctctttcgt atcccacgat
     6301 tcgtgagggc tcgatgcctg actccctgag gtgtttatca gtgagaagcc ttgtaccggc
     6361 accagattgt ctgttgataa ataccacccc gtccctcgta agatcctcaa atcccctgat
     6421 acccttcggg tttccctttt tcaccaaaaa tccctgttct ctataagtga gattgatgag
     6481 aacaatcttt ctctcgggga gaagcctgcg tatgaacggt acattgtaca gccccgtttc
     6541 ctcatcaaga aggtgagtcc ctgcaatatg cgcttcccct ttcttaagtg ctatcaaacc
     6601 acccatggaa cccacatggg cagaagaaaa ggaaaactga gggtgcctct tcttgaggat
     6661 atttgaaagg atatccagag tattgtcgtg acttccgata cagacgat
//
LOCUS       DATKOU010000198         8312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3744513, whole genome shotgun sequence.
ACCESSION   DATKOU010000198 DATKOU010000000
VERSION     DATKOU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744513"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VMU21_09175"
     CDS             complement(<1..903)
                     /locus_tag="VMU21_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUO77734.1"
                     /translation="MRIVVTSDKCSGCESCVDSCPYTAIVMQEGKAFINEYCQLCRAC
                     LGVCPEGAIKEVLEEGESPAPRESRVEDYSGVWIFAEQRGGKVASVAYELLGAGRRLA
                     DERRSELSAVLLGGSEVEAGELVRWGADRVYLSHDPIYEHFNDEPYAEMLSLLIEEHR
                     PEIVLAGATPIGRSFFPRVAAKLWTGLTADCTSLEIDKETGNLLQVRPAFGGNIMATI
                     LCPNNRPQMATVRPRVMKRGRYDEGKKGDIIHVDAQGLSSRTRVIQTVKEVSEIAVNL
                     QEVDIIVSGGRGLGDAKGFKLLEEL"
     gene            complement(894..1676)
                     /locus_tag="VMU21_09180"
     CDS             complement(894..1676)
                     /locus_tag="VMU21_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HUO77735.1"
                     /translation="MNIVVCIKQVPDTAEVRINPETNTLVREGVPSIINPYDMHAIEA
                     GLQIREKAGGKVTVISMGPPQAESALRDAISMGADEAVLLTDRAFAGSDTWATSYTLA
                     KAVEKLGADIVICGKQAIDGDTAQVGPEMAEFMNIPHISYVKKTEVIEAESIRVQRLM
                     DEGYDVVESSLPVLLTVVKELNEPRLPSLKGKMAARRAEIRKWSSSDLGADADKIGLK
                     GSPTQVKNIFAPEAKKDRKMLEGTLEEQVGALVRELGSLKCV"
     gene            1906..2760
                     /locus_tag="VMU21_09185"
     CDS             1906..2760
                     /locus_tag="VMU21_09185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUO77736.1"
                     /translation="MKAGFIGLGHLGKSVAKRLISEGIELMVWNRTREKASDLGALIA
                     DSPAALVSQVEILFMNLFDSAAVEAVLAGKDGILKVDCTGKIIIDTTTNHFELVAYFH
                     ELLRKHGGFYLESPVLGSVVPASQGALTVLVSGEKNAFDKALPYIEKIGKNIFFLEKP
                     ALATKMKLINNLALGSFMATIAEALAFGESAGINKSKVLDILGTGAGNSMVLNAKREK
                     LLQEDFSTHFSSALIYKDLHYLQDLARALKRPLFTGSTAKELFGMTFSKGMDSLDFSG
                     IYKIMKGF"
     gene            2794..3450
                     /locus_tag="VMU21_09190"
     CDS             2794..3450
                     /locus_tag="VMU21_09190"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HUO77737.1"
                     /translation="MPLSNTPISLKNYFERVTSSIVSLAITDNSTSMLSVRKKGDAIS
                     VRLHRIFLDARHDVIDEIAVFIKRRKGSTPLLRRFVKENLIRVRNAPPKRVKINTQGN
                     YHNLGEIYDSLNRDYFQEKLSCPITWGTGRARYAVRKRTLGSYSRHANIIRINTTLDR
                     KHVPRYFIEFVIYHEMLHADMDPTVKNGRNIVHSREFRGREKQFRDYERALKWEKKKI
                     "
     gene            3547..4314
                     /gene="mtnP"
                     /locus_tag="VMU21_09195"
     CDS             3547..4314
                     /gene="mtnP"
                     /locus_tag="VMU21_09195"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HUO77738.1"
                     /translation="MRGIKVGIIGGSGMDDPRLMKDIKEKRAKTPYGNPSSPLTIGKI
                     NGVDSVVLARHGKRHSIYPTGVNFRANIYALKKEGCTHILATTAVGSLREKIKPGDLV
                     FVDQFIDFTKHRPLTFHDEKVIHTPMSQPFCKELRSLLIASAKELRLRHHKEGTIITI
                     EGPRFSTKAESHMFRAWGADVINMSTVPEVILARELGICYQTIAMSTDYDCWKEGEEP
                     VTWDMIVSIMKKNTDNVRRLILNTIGKIKNTECGCKI"
     gene            4326..4859
                     /locus_tag="VMU21_09200"
     CDS             4326..4859
                     /locus_tag="VMU21_09200"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HUO77739.1"
                     /translation="MSIKSKIRTIPDYPKKGIMFRDITTLIKDPVGFRLVIDNFTQRY
                     IQGDIKFDAIVGIESRGFIIGGALSYTLGKGFIPVRKAGKLPAEKISHEYELEYGTDK
                     IEMHKDAFEKGTRVILVDDLLATGGTALAAAALVEKLGGIVSEMAFVVNLPDVGGYKK
                     LIQKGYKVYCQTEFEGD"
     gene            5352..5732
                     /locus_tag="VMU21_09205"
     CDS             5352..5732
                     /locus_tag="VMU21_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77740.1"
                     /translation="MKKKVLFFIPSLEGGGAEKVMVNILRRADKSKIESVLMLLSPIE
                     QSPYRELLPEHLRVIIVKRNSDTCIEKIKQCVKFIRVVKKENPKRILSMLTHTNILAI
                     MTGISLGIKVTVCEHNTLGELIKT"
     gene            complement(5844..8087)
                     /locus_tag="VMU21_09210"
     CDS             complement(5844..8087)
                     /locus_tag="VMU21_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUO77741.1"
                     /translation="MNLKDRVGSVFLIILFTVPMISCNGRFLSSPQSITFIGSPWYCN
                     LLHMPLAPYPALLPKPYLGIYLSSRNVEQSGFSCKENTFVQVAGVIDGMPAEEAGLKE
                     GDVILSLNGEPTCATGENVIASFRAIVEKQPIDSIVAMDVLRNDQRLTMTARLSAMPV
                     HEQSEAVHPEIGQCPDWRSMLDNALHAQDSYTRYTTTMEGLYEKSNAVHNPGSVDEKE
                     NHPLQLSEVTYMMRHPLSAGEVARELTEKLVIPLHEKDRNIGTVVHETARLIDLEMHQ
                     TERPAEISFPALLRIMEETKNNVERALNNLTPEERTVLQKKALNPWDDNAWNDVLRIS
                     MKVNRAQLFEAFSPLLTFLTRDNLSLLKEDLIHRFGKNSGPILYEAMTPVGKVIVGGP
                     GPNVYREDAALILDLGGDDLYLNNAGGTRPGMPVALVIDWEGNDRYLSRENFSQGAGL
                     LGGSFLIDMGGDDTFVSLDGSQGGGFWGIGLLYHGEGSSTYTGRTYCQGVGQMGIGLL
                     IDQKGDDRYQCSFSGQGLGLFGGVGILIDQGGNDFYQLGGLNPDFRDPSKATVSMGQG
                     FGHGIRADRGIQGVPGGIGVLIDEGGNDTYIADYFAQGASYYYGLGILDDRDGDDQYI
                     SGRYAQGAGIHSSVGVLIDRKGNDFYYASVGVAQGMGHDFGVGYFEDDEGNDQYWGGM
                     LVQGAATNGSIGIFIDLEGNDQRAFLSNGQGYSEGRGGLGIMMTKGGDSLGGAKEVTI
                     KFEVKKE"
BASE COUNT         2141 a         2164 c         1948 g         2059 t
ORIGIN      
        1 caattcttca aggagcttga atcccttcgc gtcaccaagt cctctccccc ctgatacgat
       61 aatatccacc tcctggaggt tcaccgcgat ttccgatact tccttgacgg tctgaatgac
      121 ccttgtgcgt gaagacaatc cttgggcatc aacgtgaata atgtccccct ttttcccctc
      181 atcatatctg ccccgtttca tgaccctcgg tcttactgtt gccatctggg gcctgttgtt
      241 cggacagagg atagtggcca tgatattgcc gccgaacgca ggccgtacct ggagaaggtt
      301 ccctgtttcc ttgtctattt ccagggaggt gcagtccgcg gtaagccctg tccagagctt
      361 tgcggcaacc cttgggaaga atgaacgacc tatgggagtg gcgcctgcaa ggacaatctc
      421 cggacggtgc tcctcgatga ggagtgagag catttcggca taaggctcat cattgaaatg
      481 ttcatagatg ggatcatggg agagatagac cctgtccgca ccccacctga caagctcccc
      541 ggcctctact tcagatccac ccaggagaac cgcggagagc tctgaacgcc gctcatccgc
      601 gagacgcctg cctgcgccca aaagctcgta cgcaacagac gctaccttcc ctcctctctg
      661 ttccgcaaat atccataccc cggaatagtc ttcaacgcgt gattcacggg gagccggaga
      721 ttcgccctcc tccagaacct ccttgatggc tccctcaggg catacaccaa gacatgccct
      781 gcagagctgg cagtattcgt tgataaaagc cttgccctct tgcataacaa tggcggtata
      841 agggcaggaa tcgacacacg actcgcagcc ggaacattta tcgctcgtta ccactatacg
      901 catttcagac tccccagctc cctgaccagc gcccccacct gttcttcaag ggttccctcc
      961 agcatcttcc tgtctttttt cgcttcaggg gcaaaaatat tcttcacctg tgtaggagag
     1021 ccctttaacc cgatcttgtc agcatccgcc cccaagtcag aggatgacca cttccgtatc
     1081 tctgccctcc tggcagccat ttttcctttc agggacggca gtctcggttc attgagctcc
     1141 ttgaccacgg tgaggagaac agggagggat gattccacaa cgtcataccc ctcatccata
     1201 agtctctgga cccttattga ctcagcctct atcacctctg tttttttcac ataggaaata
     1261 tggggaatgt tcataaattc agccatttca ggaccgacct gggcagtatc tccgtcaatg
     1321 gcctgtttgc cgcatataac tatatccgca ccgagctttt ccacggcctt cgcgagggtg
     1381 taggatgtgg cccatgtatc ggagcctgca aatgccctgt cagtgagaag gaccgcctca
     1441 tctgctccca tggatatggc gtccctcagt gctgactctg cctggggcgg ccccatgctg
     1501 attaccgtca ccttcccccc cgccttttcc cttatctgaa gtcctgcctc gatggcatgc
     1561 atgtcgtatg ggttgatgat actgggtacc ccttccctga cgagggtatt cgtctccgga
     1621 ttgatcctca cttcagcagt gtccgggacc tgcttgatgc agacgactat attcatgctc
     1681 ttccatcctc ccgcgtgtat gaatgcatac gtgtacccac tgcagtattg cagaaatgaa
     1741 tattatacgc cttgggcctt cccatgtaaa gaatcctgct ggtggctgcc cttctgctca
     1801 gatcacatcc ttcttatttc ttgtccggtt attttataaa tgagtatcac agaaatgtga
     1861 tagtattaat cattcgactt aatcccacct aaaggaggtg ttcgtatgaa agcagggttc
     1921 atcggcctcg gccatctcgg aaaatccgtg gcgaaaaggc ttatctctga aggaatagaa
     1981 ttgatggtct ggaacaggac cagggaaaag gcatcagacc tcggggcact gatagcagat
     2041 agccctgctg ccctcgtatc ccaagtcgag attctgttta tgaacctctt tgacagtgct
     2101 gcggtagaag cggtccttgc tggaaaagac gggatcctca aggtcgactg tacagggaaa
     2161 attatcatag atactacaac caaccatttt gagctggtcg cgtatttcca tgagctgctc
     2221 aggaaacacg ggggattcta ccttgaatcc cccgtgctcg gcagcgtggt tcccgcgtcc
     2281 cagggagccc tcaccgtcct ggtgagcggt gagaagaatg cttttgacaa agcccttccc
     2341 tacatcgaaa agatcgggaa gaacattttc ttcctggaaa agcctgctct ggcaaccaag
     2401 atgaaactca tcaataacct tgcactcggc tcattcatgg caaccattgc agaggccctt
     2461 gcattcggtg aatccgcggg cattaataaa tcaaaggtgc tggacatcct tggcacaggg
     2521 gcaggcaact ccatggtgct caacgcgaag agggaaaagc tccttcaaga ggacttttca
     2581 acccatttct catctgcatt gatatacaaa gacctccact acctccagga tttggcaagg
     2641 gcattgaaga ggccgctttt taccggaagc actgcaaaag agctcttcgg gatgacattc
     2701 tccaaaggca tggacagcct ggatttttcc gggatataca agatcatgaa ggggttttag
     2761 aacctgacca gacagggccc tgaacaactg cctttgcccc tctcgaatac tccgatttcc
     2821 ctcaagaact attttgagag ggtcacatcc agtattgtct ccctggctat caccgacaac
     2881 tccacgagca tgctttcggt gaggaaaaag ggggatgcca tttctgtcag gctccatcgt
     2941 atcttcctgg atgcccggca cgatgtcatt gatgagattg cagtattcat caagagaagg
     3001 aaagggagca cccctctcct caggagattc gtgaaagaaa acctcattcg agtaagaaac
     3061 gcaccgccca aaagagtaaa gatcaacaca caggggaact accacaatct tggtgagata
     3121 tacgactcgt tgaacaggga ctactttcag gaaaagctct catgtcccat cacctggggt
     3181 actggcaggg caagatacgc ggtaaggaag aggacgctgg gaagttacag caggcatgcc
     3241 aacatcataa ggataaatac aaccctcgac agaaagcacg tgccacgcta tttcattgag
     3301 ttcgtcatat accatgaaat gctccacgct gatatggacc ctactgtgaa aaacggaagg
     3361 aacatcgtgc attcaagaga attcaggggc cgggagaagc aattcaggga ttatgaaagg
     3421 gcattgaaat gggagaagaa aaagatttga tatgtggatg acgaatctgt gaagcttgac
     3481 tttggttggg ggtattatat attttgtaac tcaaccttct ttcacgaagg cacgaaagga
     3541 gattttatgc gcggcataaa ggtgggtatt ataggcggat cagggatgga tgacccgcgc
     3601 cttatgaagg atataaaaga aaagagagcg aagacccctt atggaaatcc ttcctctccc
     3661 ctcaccatcg gcaaaataaa cggagtggat agcgttgtgc tcgcccgcca tggcaaaaga
     3721 cattccatct atcccacagg cgtgaacttc agagcgaaca tttacgccct caagaaagaa
     3781 ggctgcaccc atattcttgc caccaccgcg gtcggttcgc tcagggagaa gataaagccc
     3841 ggagaccttg tgttcgtgga ccagtttatc gacttcacaa agcaccgccc tctcacattc
     3901 catgacgaaa aggtgataca cacgcccatg agtcagccgt tctgtaagga gctgcgctcc
     3961 cttttgatcg cttctgcaaa agaacttagg ctgagacatc ataaggaggg aacaataatc
     4021 accatcgagg gcccgaggtt ctcgaccaag gcagagtccc acatgttcag ggcatggggc
     4081 gcggatgtca taaatatgtc gaccgttcct gaggtgatac tggcaaggga gttgggtatc
     4141 tgctaccaga caatagccat gtcaaccgat tatgactgct ggaaagaggg agaagaaccg
     4201 gtcacgtggg acatgatagt ctccataatg aagaaaaaca cagacaacgt gagaagactg
     4261 attttgaaca caataggaaa aatcaagaat accgaatgcg gatgtaagat atagaggggg
     4321 aaacaatgtc tataaagtca aagataagga ccatccctga ctaccccaag aagggcatta
     4381 tgttcaggga catcaccacg ctcatcaagg accctgtggg gttcagactg gtcatcgaca
     4441 atttcacgca gcggtatatc cagggggata tcaaatttga tgcaatcgtg ggaatagagt
     4501 caagaggctt catcatcgga ggggcccttt cctacaccct gggcaagggg tttatccctg
     4561 tgagaaaagc gggaaagctc cccgcggaaa agataagcca tgaatacgaa cttgaatacg
     4621 ggactgacaa aatcgaaatg cacaaggatg ccttcgagaa ggggacaagg gtgattttag
     4681 ttgatgacct cctcgccaca ggcgggacag cccttgcggc agctgcgctt gtggaaaagc
     4741 tggggggcat cgtctcagag atggcctttg tggtgaacct tcccgacgtc ggcggctaca
     4801 aaaagctcat ccaaaaggga tataaggtgt attgccagac agagttcgag ggagactaag
     4861 gggacggttg cttgttctgg tcatcccagc gactcggtct aggccttaag tcttaattcg
     4921 taaccttcac gccgaactgc gcataggctt tttgtgcgtt ccacctgaga acctgcactg
     4981 tctccgtgct ggcggttaat gcgggatccg acacttttaa gaaaattgcg cttaacaaga
     5041 cttcatccag aaaacgttgc ctttggttct tattgccaca taatacgaat gtcccgaaaa
     5101 gtggcttttt aatttgacac attttttcat tcccgtgtct tatgtttgtg ctatatttat
     5161 ggaatagggg gtattaaggg gggaacttcg tgtaatcatt tgttgaccta aaaatgatca
     5221 gacatgcgga gttctatgct atttctgaaa cccattaatc ttgaagaaat cgacagggca
     5281 agagcccgca ggggctttgg ttttgatgcg tcaatgaagc aatgtgaagt cactctgaac
     5341 ctcatagaag aatgaaaaag aaggtcctgt tttttattcc ctcgcttgaa ggtggcggcg
     5401 cagagaaggt gatggttaat attctcagac gagccgataa aagcaagata gaatcagtcc
     5461 ttatgttgct ctctccaatc gaacaatccc cttaccggga attattgccc gagcatttaa
     5521 gggttattat tgtaaagaga aattcagata cctgtattga aaagatcaag cagtgcgtaa
     5581 agttcattcg cgtcgttaag aaagagaacc caaagaggat attgagcatg ctgactcaca
     5641 ccaacattct tgccataatg acggggatct ctttgggaat taaagtaact gtttgtgagc
     5701 ataacaccct cggtgaactc ataaagacat aggcattgtg aattagtatc tcattgtagt
     5761 agagcttttg gggaatccca aaaggtgcag gacccggagg agattttgaa attccaaaaa
     5821 ctatacagat cttgataggc cttttattcc ttcttcactt cgaacttgat agttacctct
     5881 ttggctcccc ccagagaatc acccccttta gtcatcataa tccccaggcc gcctctcccc
     5941 tcagagtatc cctgtccatt ggagaggaat gcacgctgat catttccctc cagatcaata
     6001 aaaataccta tacttccatt ggtggctgct ccctggacca gcattccgcc ccaatactga
     6061 tcattcccct catcgtcctc aaaatatccc acgccaaaat cgtgtcccat gccttgggct
     6121 acgccgacgg aggcatagta aaagtcattc ccttttctgt cgatgaggac acctaccgac
     6181 gaatgaatcc ctgcaccctg cgcatagcgc cctgagatgt actggtcatc gccatccctg
     6241 tcatccagga tcccaagccc atagtagtag gacgcaccct gagcaaaata atcggcaata
     6301 taggtatcgt ttcccccctc atcaatgaga acccctatcc ctccgggaac tccctgtatc
     6361 cccctgtccg ccctgatacc atggccaaag ccttgcccca tgctcaccgt agcctttgag
     6421 gggtcgcgga aatccggatt taagcctccc aattgataga aatcattacc tccttgatct
     6481 atgagaatgc ccacgcctcc gaaaagaccc aagccttgtc cgctaaagga gcactggtac
     6541 cgatcatctc ccttttgatc aatcagcagc ccaatgccca tctgacctac accctgacaa
     6601 taagtccttc cggtatatgt gctgcttccc tctccatgat aaagaagacc gatcccccaa
     6661 aaacctcctc cctgacttcc atccaatgaa acgaatgtat cgtcaccgcc catgtctata
     6721 aggaagctgc ctcccaggag tcccgcaccc tgggaaaagt tttccctgct gaggtaacga
     6781 tcattccctt cccagtcaat tacaagcgcc accggcatcc ctggcctcgt acctcctgcg
     6841 ttattaaggt aaaggtcatc accgccgagg tccaggatca gggcagcatc ttccctgtat
     6901 acattcggcc ccggaccgcc cacaattacc tttcccacag gggtcatggc ctcatacaaa
     6961 atcggcccgc tgtttttccc aaatctgtga ataaggtctt ctttgaggag cgacagatta
     7021 tcccgggtta aaaaagtgag caagggagaa aatgcctcaa aaagttgtgc gcgattcacc
     7081 ttcatcgaaa ttcttaagac atcattccac gcattgtcat cccatggatt taatgccttc
     7141 ttctgcagta ctgtcctctc ttccggggtc aggttgttta acgccctttc cacattgttc
     7201 tttgtttctt ccatgattcg gagcaacgcg ggaaagctga tttcagcagg tctttcagtc
     7261 tgatgcatct ctaaatcaat gagtcgggcg gtctcgtgta ccacggtgcc tatgttccga
     7321 tccttctcat gaagaggtat aacaagcttc tccgtaagtt cccttgccac ctcccctgct
     7381 gacaggggat gacgcatcat ataggttacc tcgctcaact ggagtgggtg gttctctttt
     7441 tcgtccaccg atcccgggtt atgcacagcg ttggatttct catagagccc ctccatggtt
     7501 gtggtatatc ttgtatacga atcctgcgca tgcagcgcgt tgtcgagcat ggagcgccaa
     7561 tccggacatt ggccgatttc cgggtgtaca gcttcgcttt gttcatggac cggcattgcc
     7621 gaaagcctgg cggtcatagt cagtctctgg tcattacgca gaacatccat ggccacgatc
     7681 gagtcaattg gttgtttttc gactatagcc ctgaatgatg ctatgacatt ttctcctgtg
     7741 gcgcatgtgg gttcaccatt aagagaaaga atcacgtcac cctctttgag ccctgcctct
     7801 tctgcgggca ttccgtctat aacccctgcg acctgcacaa acgtattttc tttacaggaa
     7861 aaacctgatt gctccacatt tctggaagaa aggtaaattc cgagataggg tttcggaagc
     7921 agtgcaggat aaggcgctaa aggcatgtgc agcaggttgc aataccacgg ggaccctatg
     7981 aacgtgatgg attgtggtga agacaggaat cttccgttgc acgaaatcat gggtacggta
     8041 aagaggatga tcaagaaaac actgcccact ctgtccttaa gattcatgta tctcctcatt
     8101 tgtgatcact attgtatacc aggtcgcggc atctttaaat aataattgac agattctgcc
     8161 aaaagttata cattaaatta accattggat cccataaccc cgctgggatg atgagggtat
     8221 tggtacgggg aggattaacc gagcatatcc cttttctccg catcacactg atggggagat
     8281 ccagaaggag gccttagatg aaaagaatac gt
//
LOCUS       DATKOU010000199         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2195064, whole genome shotgun sequence.
ACCESSION   DATKOU010000199 DATKOU010000000
VERSION     DATKOU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195064"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VMU21_09215"
     CDS             complement(<1..646)
                     /locus_tag="VMU21_09215"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HUO77742.1"
                     /translation="MNIDLMSEVLKRVLSRGGDYADIFVEQRRSSSIRLEDGKVEKVV
                     TGIDSGVGIRLISRGKTSYAFTNDSSPDSLTSLADVLSRSLRGVASSSIPDLKRVSPE
                     SDFPIRLLPDEVPMERKVALVQKADHIARSFDKRIRQVSASYCDATQNVLIATSEGLF
                     AEDERTHTLWVIHVIASDDGIIQTGYETAGGNVGFELFRGSIIDELSLKASQRAV"
     gene            772..1554
                     /gene="mtnP"
                     /locus_tag="VMU21_09220"
     CDS             772..1554
                     /gene="mtnP"
                     /locus_tag="VMU21_09220"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HUO77743.1"
                     /translation="MPKIGLITGSGFYEEGMGIRELRKITTPYGEPSDVYRVGEFPEG
                     EFVLLSRHGTPHHIPPHMINYRANIWGMREMGVERIISINAVGGIEGGLAPGDVVIPD
                     QVIDMTHGRAATFYEGDEVVHIDFTTPYCAEVRGAIKASGIESRVSLRDSGTYVCVNG
                     PRLESKAEVQHFLKIGADIVGMTVMPEACLARELQLCFACIAVVTNYAAGISDRKLTT
                     KEVVETMKGSLERIKKLIMETFKHIPEKRGCECPHALKDTTM"
     gene            1584..1811
                     /locus_tag="VMU21_09225"
     CDS             1584..1811
                     /locus_tag="VMU21_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUO77744.1"
                     /translation="MKVTVKLFATLRNFGPVEQEMELAENSTLEDLIKMLKLPEKIPL
                     LKIVNGEHRSMHYSLREGDEIALFPPIAGGS"
     gene            complement(1871..2389)
                     /locus_tag="VMU21_09230"
     CDS             complement(1871..2389)
                     /locus_tag="VMU21_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77745.1"
                     /translation="MGGETTPKEQRINSQECHLEISLRAVPSRIGTSGGKEVPFKRGV
                     LSLRNTGQTVVSGLVPYATLGQEGGSIQDVNEAFSRSKTVESIKPGEAIEWDVYDQLL
                     TAHAGAASKVHMFGYRAVLNWWFELVAWAEYRCPDVATSLQTPITRWKLRWSLAKPPM
                     DEVDLYIEVIKE"
BASE COUNT          644 a          642 c          671 g          629 t
ORIGIN      
        1 taactgccct ctgggaagcc ttgagggaaa gctcgtcgat gatggatccc ctaaaaagct
       61 cgaaaccgac attgccgccc gctgtctcat accccgtttg tattatccca tcgtctgagg
      121 caattacgtg gattacccag agggtatggg tgcgctcatc ttccgcgaag aggccctcag
      181 aggtggctat caggacattt tgggtagcat cacagtaact tgcagacacc tgccgtatac
      241 gtttgtcgaa tgaccgggct atatgatccg ccttctgcac aagggcaacc ttcctctcca
      301 tcggtacttc atcagggagc agcctaatag gaaaatcaga ctctggactt accctcttaa
      361 gatccgggat agaagaggaa gcgacccccc ggagggacct gctcagaacg tctgccagtg
      421 aggtgaggga gtcaggtgaa ctgtcattag tgaaggcata ggaggtcttc ccgcgagata
      481 tgagtctgat gcccacacca ctgtcaatgc cggtcaccac cttctctacc tttccgtctt
      541 caaggcgaat ggagcttgac cttctctgct ccacgaaaat atcggcatag tctcctccgc
      601 gggacaatac cctctttaac acttctgaca ttaggtcgat attcaacgca atggacctct
      661 tttttgttgt ggtactggac ttcgcagccc gtatattata gcatttagaa agatagggaa
      721 tacggctgta tagctacgtc agcagccttg cggaaggaga agagcgagtc tatgccaaag
      781 attgggctta ttacgggcag tggcttttat gaagagggga tgggaataag agagctcagg
      841 aaaataacga ccccatacgg agaaccgtcc gatgtgtacc gtgtaggtga gtttccagag
      901 ggtgaatttg ttcttctttc caggcatggg acgccccacc atatccctcc ccatatgatc
      961 aattaccggg ccaacatctg gggtatgagg gaaatggggg tggaaaggat tatctcaatc
     1021 aatgcagtgg ggggtataga gggaggcctg gcaccagggg atgtcgtgat acctgatcag
     1081 gtcattgata tgacccatgg aagagccgcc acattttacg agggagatga agttgtccat
     1141 atagatttca cgactcctta ctgcgcagag gtgaggggag cgattaaggc atctggcatt
     1201 gaatcccgtg tgagcctgcg tgattccggc acgtacgtat gtgtgaatgg gccgaggctt
     1261 gagagtaaag cggaagtcca gcatttttta aaaattggag cggatatcgt cgggatgact
     1321 gttatgcccg aggcctgtct tgcccgtgag cttcaactct gctttgcgtg catcgcggtg
     1381 gtaaccaact atgcggccgg gatatcggac aggaagctta caacgaaaga ggttgtggag
     1441 acaatgaagg gctctctgga gcgaataaag aagctcatta tggaaacctt taaacacatc
     1501 ccggagaaga ggggttgcga atgcccgcat gcgctgaaag atacaacgat gtaacgggct
     1561 ctccggtata atttatctgc gctatgaagg tcactgttaa actttttgcc accctgagga
     1621 actttggtcc tgtggaacag gagatggagt tggccgaaaa ctcaaccctt gaagacctga
     1681 tcaagatgct gaaactcccc gagaagattc cgttgctgaa gattgtgaat ggtgaacaca
     1741 gaagcatgca ctattccctg cgggaaggag acgagatcgc actctttcct cctattgccg
     1801 gaggttcttg aaggtacaca tatcgcgtga gaaggccgac gagacgaaac aatttacgta
     1861 tgcgcgtgct tcactctttt ataacttcga tatacagatc aacctcatcc atcggaggct
     1921 tggcaaggct ccagcgcagc ttccaccggg tgatcggcgt ctgtagagag gtagcaacat
     1981 ctgggcaccg gtactcagcc caagcgacta attcgaacca ccagttcaag acagccctgt
     2041 atccgaacat atgaaccttg ctggcagcac ccgcatgtgc ggtaagcaac tggtcgtaca
     2101 cgtcccattc gattgcctct ccaggcttga ttgactccac ggtcttggag cgggagaatg
     2161 cctcgttcac atcctgaatc gatccgcctt cctggcccag ggtggcgtaa ggcacaagcc
     2221 cagacaccac tgtctgaccg gtattcctta acgaaagaac ccctctcttg aaagggacct
     2281 ccttaccccc gctagtccca attcttgaag ggaccgcacg caaagagatc tctagatggc
     2341 attcctggct gtttattcgc tgttctttag gtgttgtttc tccccccatg ctctcctcct
     2401 ttccactcat cttttatgga gtggatcaat cagtagttat aactgaaagg tataccagat
     2461 catgtctgga caaccagttt tctttttcca gaaggtcacg tctcacaggt tttttattga
     2521 gaagaatctc tttgcatccc tgccagaact ccaaaggctg acctgagaga aaatccgcca
     2581 tctggg
//
LOCUS       DATKOU010000200        26723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5667736, whole genome shotgun sequence.
ACCESSION   DATKOU010000200 DATKOU010000000
VERSION     DATKOU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 26723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26723
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5667736"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VMU21_09235"
     CDS             complement(<1..346)
                     /locus_tag="VMU21_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77746.1"
                     /translation="MDRPYKAVSGKTYSLFGVDVSTDQYFSTIHAIAESLLVLCPDQQ
                     VLLSHVQKAGGMRFRKSFAYAYDDALIAHIRKNLYEALHTYTRGVQEHLKSIPISQRF
                     DKTIRTREEGYHV"
     gene            complement(473..1495)
                     /gene="dinB"
                     /locus_tag="VMU21_09240"
     CDS             complement(473..1495)
                     /gene="dinB"
                     /locus_tag="VMU21_09240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HUO77747.1"
                     /translation="MRRILHMDMDAFFAAVEEKRHPELRGKPVVIGGSGDPTKRGVVS
                     TASYEARKYGIHSAMPLQTAYKLCPHAIFLPVDYEEYARVSSILKELLKGVSPVMEDV
                     GIDEAFLDISEIQKTSELVAMEIKEKITAHTGLTCSIGIASNKLLAKIASDLQKPDGL
                     TILTADDVESRIWPLPVRKLWGVGPKTEGYLKEMSIHTVGDLAKIPLDTLIEHFGPSY
                     GNYLHDSSLGIDRTPLITDWEPKSASRETTFQADTNSWQTIARSLAELVKDVVDDIKG
                     REYKGKHVTVKIRFSDFQTQTRACTLKEPTDNVELIRKTAFLCLGKFELKKKVRLVGI
                     RVGLSK"
     gene            complement(1718..2863)
                     /locus_tag="VMU21_09245"
     CDS             complement(1718..2863)
                     /locus_tag="VMU21_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HUO77748.1"
                     /translation="MPILLSVPAIALIVLVLWEAFEVILLPRRVTRRYRFTRFFYRNA
                     WRIWSAVFRSISKGGRQETFLSYFGPLSLLLLLSIWAYGLIVGFALLHWSLGSPIRTP
                     EGTAGFSTDLYLSATTFFTLGLGDVTPVVPLARVFTAVEAGLGFGFLALLFAYLPALN
                     QSISRREVNISLLDARAGSPPTAAEMLRRRCHDSDNMETLLQHLSEWERWAAELLESH
                     LSYPVLAYFRSHHDNQSWLAAITAILDTSAFVVSYMEGSCARQAMLTFAMARHAVVDI
                     SLIFGRAPFNPKRDRLTPEELTELRTALASAGLPLREGDEAEKELTELRQMYEPYVNA
                     LSQHFQLPVPPWIPESHRRDNWEMSAWERRKRLQKTERAGKEEDEHF"
     gene            3178..3366
                     /locus_tag="VMU21_09250"
     CDS             3178..3366
                     /locus_tag="VMU21_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77749.1"
                     /translation="MDDSELKDTVELLCPACGAVLTLEVRTEDGWNCQCGDFIPDGLA
                     INPSQGCSCQHKQNCSWR"
     gene            complement(3391..3735)
                     /locus_tag="VMU21_09255"
     CDS             complement(3391..3735)
                     /locus_tag="VMU21_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HUO77750.1"
                     /translation="MRQLGIEGEELAVRFLKKKGYKIVSRNFKSPVGEMDIIAEDGET
                     LVFVEVKTRTDDSFGHPFEAVTHRKREKLRKVALSYLKHSRREVASRFDVLSIETDGD
                     IHKIEHIKDAFE"
     gene            complement(3716..4189)
                     /locus_tag="VMU21_09260"
     CDS             complement(3716..4189)
                     /locus_tag="VMU21_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HUO77751.1"
                     /translation="MIKSHSRQPHKDRGPFKVHAQGPTFGDMERASRLREVLEALYEV
                     NKKFPVIVEGKRDAMALRRLGLAGEVIVLHSGNNLYEFSEDISERFERVVILLDWDEK
                     GEHLSKTIQKHLRGQWEEFSAFREILKILCQKDIKDIEGIPKLLGKLEGDEAAGH"
     gene            complement(4243..5253)
                     /gene="trpD"
                     /locus_tag="VMU21_09265"
     CDS             complement(4243..5253)
                     /gene="trpD"
                     /locus_tag="VMU21_09265"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HUO77752.1"
                     /translation="MIKEAIHLLVQSIHLSEAEMAECMKEIMEGRATEAQVAAFLTAL
                     RIKGETVEEITGAARVMREKAATIKAPEGVLDTCGTGGDMAHTFNISTAAALVTAAAG
                     VPVAKHGNRSVSSQSGSADVLEALGVRIDLPPDKVERSLYETGFGFLFAPLFHPAMKY
                     AVGPRKDIGIRTIFNILGPLTNPAGAKRQILGVFADRLTDTLAAVLGNLGAEDIMVVH
                     GEDGLDEITISDGTKVSRWKAGRVETFYITPEDFGFRKADVQSLSGGDKHENARILHS
                     LFNGEKGPKRDVVLMNTAAALVVADKARSFEEALPMASEVIDSGKATQKLQEIIKITN
                     AL"
     gene            complement(5250..5825)
                     /gene="pabA"
                     /locus_tag="VMU21_09270"
     CDS             complement(5250..5825)
                     /gene="pabA"
                     /locus_tag="VMU21_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HUO77753.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGEEIRVFRNDAITIQEIRELGP
                     ERIVISPGPCTPNEAGISVEVICKFAGKIPLLGVCLGHQSIGAAFGGEIIRAPRLMHG
                     KTSTIHHDGRTIFKGLSNPFEATRYHSLLIRRETLPQCLEITAWTDRDEIMGVRHREF
                     IVEGVQFHPESILTTEGKALLKNFLKLGKRR"
     gene            complement(5826..7337)
                     /gene="trpE"
                     /locus_tag="VMU21_09275"
     CDS             complement(5826..7337)
                     /gene="trpE"
                     /locus_tag="VMU21_09275"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HUO77754.1"
                     /translation="MLYPALNEFKALCAKGNLIPVYREILADMDTPVTAFLKLGGTPS
                     FLLESVVGGEKWARYSFIGSRPSKVIMAWGRRVEIREEGKPPMVFDSDDPLEVLKKEV
                     SRYVPVEVRGIPRFFGGLVGYMGYDMVRFFEKIPDRSREGLGLPDMFFMLTDTMLIFD
                     SLQQKIKVVSNAHIEGKKPDEAYHEAEDKIREIIERLNQTSVTVPPPAVAGRAQGVRE
                     GKKRMNGSFTSSFGTREAFKEAVSKAKEFVMAGDVVQVVLSQRFERELSVDPFNVYRA
                     LRVINPSPYMYYLDTGEARIVGSSPEILARLEGKTVVLRPIAGTRRRGETESEDKELE
                     EELKKDPKEIAEHIMLVDLGRNDVGRVARTGSVKVTELMSVERYSHVMHLVSHVEGEL
                     REGLDAFDVLRATFPAGTVSGAPKVMSMEIIEELEPTRRGPYAGAVGYFSYSGNMDTC
                     ITIRTLIIKDQKVYVQSGAGIVADSDPEGEYGETVNKAIAMMKAVDMAEKDFE"
     gene            7419..7494
                     /locus_tag="VMU21_09280"
     tRNA            7419..7494
                     /locus_tag="VMU21_09280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7452..7454,aa:Gly,seq:gcc)
     gene            7636..7711
                     /locus_tag="VMU21_09285"
     tRNA            7636..7711
                     /locus_tag="VMU21_09285"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7669..7671,aa:Cys,seq:gca)
     gene            complement(7690..9543)
                     /locus_tag="VMU21_09290"
     CDS             complement(7690..9543)
                     /locus_tag="VMU21_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04442 family protein"
                     /protein_id="HUO77755.1"
                     /translation="MVKDFMLHGSIGPVDFFVFMGGANAGNTYFYEEDGPRIRFFSRG
                     NELTLAPEGVHYKGTGGSFCEYMFGVEKPFKDLVKNEVLNRLVMFGAFLDKDERLIFT
                     NDTTGSESFQRLFLLGHAVKNYYFFISSDDRNEPRKRQRHIVRSLGKFLKRTDLVAED
                     NDGELLSGFVKELSEPRSTVFIFKLVQSENLEFYKAFHAAYSLKRALTPEEEARLNGI
                     AANYQIDDYQQERMKIDSMYRHHENRNIVDEYRDILLDIAYKETIQNSEIAKLHRLRT
                     LSIRNNIPGILFDTLDEFLLKDKDVQAIEEPEYLKESRAILENLFFKSPSLKRHIIKE
                     DIARLIWAKHSSHSQSDMGFERVLLDIGKSCDEFAREKNDFSIFEELSAIITYFDRYD
                     NVHSSLSRIAFMEHPELSEDSLRSLVGNKKEFDALDKNLFKEIFISDLLSNRYITSFG
                     KKKIKVLHEHIEKVLRGDASLRDLIVALEGIAEEEKIYKYVRSALKERMHELYTSLNM
                     KKEMMRIRGEIEAELVGKGTNLKLYEQLFNRVLLDLKKESYYLNHLLPAITKNRDMAL
                     REDFLNNSGLDRFYIEGLEKEYLKDDEMGPLILEWEREDKEFSGTGGGERI"
     gene            complement(9747..10127)
                     /gene="queF"
                     /locus_tag="VMU21_09295"
     CDS             complement(9747..10127)
                     /gene="queF"
                     /locus_tag="VMU21_09295"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HUO77756.1"
                     /translation="MLRYGEKAIRKAKLQTWDNPSAQRDYEITISFPEFTCLCPRSGY
                     PDFASITITYVPDKKIVELKALKLYLNSFRDVHTSHEEVTNRIYSELEKVVRPRFLEV
                     TGDFNPRGNVKTLVRVCSEDRTRP"
     gene            10367..11482
                     /locus_tag="VMU21_09300"
     CDS             10367..11482
                     /locus_tag="VMU21_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUO77757.1"
                     /translation="MYVEKESATSAQYVPSPVATKAIDRNISLFTERIKERFSLYLER
                     SGKYLALMKEILKAKNMPEDIAFLPLIESGFNPNAYSVARAVGPWQFIASTARNYGLT
                     IDWWRDERKDPVKSTEAAANYLKDLYEMFGSWNLAMAAYNAGEGKILRAINRTKSDNY
                     WALLNTRHIKKETKEYVPRFIAATLIATNPHDYGFADLDYLPPLRYDEVVLDRPVDLD
                     IAARCAETSLDMIRELNPEIRRWSTPANVSSYALRIPFGKTEIFTENLSEIPEEERFS
                     ISTYTVKKGDTIKTISTKTGIPINVIQELNDNGNGLKTLKAGKTISLPPKEKLCFDRD
                     DREGKRSAKNRRSKGRKQANKKIVVTSLSKKQAYIDD"
     gene            complement(11504..12208)
                     /locus_tag="VMU21_09305"
     CDS             complement(11504..12208)
                     /locus_tag="VMU21_09305"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO77758.1"
                     /translation="MPKAIKKKVTKPVKTEEGMMSIIFRVREMIAERQKLFFLVVVSC
                     VILCLALAGFFMYRSNARERAGALEYEGYKLYYGMFQKQPLQKEGRYQAALEKFRKAY
                     ELRKGPYSLFYVANCYYEMGKDDEALKALKELNERFPDDERFVPLTYYKMAVINLRKG
                     DREAALKLLNTLSNYKTASFKDLALIESGRILESMGQTAEAAQKYEEITKNFPASPFA
                     EEARIKLSGKKTPEKS"
     gene            complement(12208..12867)
                     /locus_tag="VMU21_09310"
     CDS             complement(12208..12867)
                     /locus_tag="VMU21_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HUO77759.1"
                     /translation="MMIDLHCHTLFSDGELLPYELVRRAEAMGYNAMALTDHVDPSTI
                     DFVVPRIVKALKKLREVVSIEVLPGAEITHAPPRLIHELVKEARSLGARVVLVHGETI
                     TEPVAEGTNKAAIEAGADILSHPGLITTEDVLYAKDKGVILEITARKGHSLANGYVAK
                     EAIKFGVPLCLNTDTHGPGDLITEETARRILLGAGVEESRIESVFENSRLLVEKALRR
                     D"
     gene            13144..13794
                     /locus_tag="VMU21_09315"
     CDS             13144..13794
                     /locus_tag="VMU21_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HUO77760.1"
                     /translation="MSIGLFQRISDIDLQYCIKNNLPVVRRPTGGRAILHGDELTYSF
                     SSGNEGFFSGGLLDTYRQLGSALKSALDMLGLDITIKRERAEGRTLTRSPLCFRSVSY
                     GELSWEGKKLVGSAQKRWKDGFLQQGSLPYLIDEENTRKVFSIPSSSDIKKGMIGLRE
                     AVPGIEPRQIKEAIRASFERSFRISLLYSGLSPEEFHLARELQVQKYVSPEYTLQR"
     gene            complement(13694..14734)
                     /locus_tag="VMU21_09320"
     CDS             complement(13694..14734)
                     /locus_tag="VMU21_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77761.1"
                     /translation="MNYTEAIKSGFRIVNKNWQLVLIQVCAMGASFMSFFVVVGVPLA
                     IAFIIFGLDLTELSRIQDALRTFKEPAEILTKYFALVVLVLASLLLYVLVVLVIGIFL
                     LGGSMGVIGRWLKGEQEKFRMKVFIAEGRQRFFPLTGFTTMVGLLFVAVAFILGLFGG
                     AIAAIVSVAREQEATLALFLGIFFSLILFVVGMVLIFLTLSITIYGAAIITLRGMGPF
                     QSLKESIRYLYRSPEGLYLYCVIFCGYIAISFLIIFLSYPIGSVPFIGPLIALVYHFC
                     LYIMQSYLGLVMIAAVFWYYQRATGVLKEPPGPSLPLTTAEECTPEIHTSEPEAPGQG
                     EIPQEKDQNKEG"
     gene            complement(14831..15445)
                     /locus_tag="VMU21_09325"
     CDS             complement(14831..15445)
                     /locus_tag="VMU21_09325"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HUO77762.1"
                     /translation="MTVKVKICGITNVDDALAASDVGVDALGFVFHKESPRHVSPDQA
                     REIVRRLPAFITTVGVFVDMPLGEVEAIMHHASLDVIQLHGHELPEACVLGRRVIKAI
                     RVKELSDLEPLKNYRVSAFLLDTYTQESFGGTGQIFNWDIALDAKQFGKIILAGGLNP
                     DNVDKAVRWVKPYGVDVSSGVEERKGKKDQGKMRLFIERAKGAS"
     gene            complement(15488..16843)
                     /locus_tag="VMU21_09330"
     CDS             complement(15488..16843)
                     /locus_tag="VMU21_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HUO77763.1"
                     /translation="MKETKIVLPDREIPKQWYNIMADMPNLPKPPLHPGTLKPVGPDD
                     LSAIFPMALIEQEVSTQRWIDIPEEVLDIYSLWRPSPLYRAHRLEKALGTPAKIYYKY
                     EGVSPAGSHKPNTSIPQAYYNKKAGIKRIATETGAGQWGSSMALAGSFFGLEVTVYMV
                     KVSYDQKPYRRIAMETWGATVHPSPSTVTNSGRKILELDPESQGSLGIAISEAVEDAA
                     THSDTNYSLGSVLNHVLLHQTVVGLETKKQFALVGDYPDVIIGCCGGGSNLGGASFPF
                     LLDKIHGKDLRVIAVEPISCPTLTKGEFRYDFGDTAGLTPLLMMYTLGHDFMPPGIHA
                     GGLRYHADAPLLCQLYHDRLIDAVAYGQTAVFEAAVTFAKAEGIIPAPETAHAIRATI
                     DEAMRCKEEGKQRTIFFNLSGHGYLDLTAYADYLSGKLEDYAYPDDKIKESLAKLPVI
                     G"
     gene            complement(16898..17689)
                     /gene="trpC"
                     /locus_tag="VMU21_09335"
     CDS             complement(16898..17689)
                     /gene="trpC"
                     /locus_tag="VMU21_09335"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HUO77764.1"
                     /translation="MSILNRITEKKRERLIHAKGAAPLRDLKARIPDTERPRDFRTAI
                     RRDEGHIRFIAEIKKASPSGGIIREDFNLAGIAHIYEEKAVDAISVLTEEDFFLGNIR
                     NVPVVKRAVTRPILRKDFIIDEYQIYESRVYGADALLLIASLLGMSQAEEYFHLCAEL
                     GLFVLFEVHSLDELEKAMRLDADIIGINNRDLNTLSVDLHTTSSLKKEIPSGRIVVSE
                     SGIKTRNDMLSLDTSGVDAVLIGTSLMTSRDIGKKLDELRGLTEQ"
     gene            17807..19381
                     /gene="murJ"
                     /locus_tag="VMU21_09340"
     CDS             17807..19381
                     /gene="murJ"
                     /locus_tag="VMU21_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HUO77765.1"
                     /translation="MDTKGTIARSAGIISLATFISRVLGYVKDMILARYFGATGISDT
                     FFVAFRIPNLLRELFAEGSMSSAFIPVLTEYQTNQGAGEAKRLVRITFTFMLICAGLF
                     CVAGVIFAPQLVALIAPGFLNNPGKFSQTVLLTRIMFPFLLFISLAALVMGALNTRRV
                     FFVPALAPAMLNVTIILVVLTLVPLLQQPILAVAVGVTLGGLVQCGFQLPVFFQRGYD
                     LRTDYRFRHPGLKKMSILMMPATMGMAVAQVNIFVSTILASYLSQGSITYLYYSMRLI
                     QFPIGIFGVAMGMAVLPTLSEHAVRGEMDKLREDFSFALRLLFFITVPAMVGLIALRV
                     PVVNILFQRGMFDYGATVGTADALLFYATGIWAIVGVRIVTATFYSLNDTRTPVKVAA
                     AAVVINIIMSLLLMNPLKHSGLAFANAIASSVNFILLFYFLRRRLGRIDAKRILRSFT
                     KICVASIIMGVLGWRVLHGEMWMASGRVVEKALYLSGTIVVCVGIYLTINFLLKGDEL
                     NYLYDRIKDRLRRSAA"
     gene            19378..20004
                     /locus_tag="VMU21_09345"
     CDS             19378..20004
                     /locus_tag="VMU21_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUO77766.1"
                     /translation="MIVRSIPLGPLEANCFIVADEHSRKAMVIDPGDEPDTIMDVLKT
                     ENLSLEYIICTHAHFDHVGAVPEIKEQTGATIVIHKDEQEIYHGARDMAAFWGYTLDP
                     LPEPDLLAEDGHEITMGSLTFTVLHTPGHSPGGICLFGEGIVITGDTLFAGSVGRTDF
                     HGGDTRKLKQSFQRLMSLPPETVVLAGHGPNSTIGRERSENFFPKLFT"
     gene            20143..20457
                     /locus_tag="VMU21_09350"
     CDS             20143..20457
                     /locus_tag="VMU21_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUO77767.1"
                     /translation="MGNKLYVGNISFQASEEDLKDLFSKAGEVLSAKLITDAATGRAR
                     GFGFIEMSSDAEAQKAISTLNGTSFMDRNIIVSEAKPQERRERGTRDRGGFGSRRPGG
                     RR"
     gene            complement(20486..21334)
                     /locus_tag="VMU21_09355"
     CDS             complement(20486..21334)
                     /locus_tag="VMU21_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HUO77768.1"
                     /translation="MRVHLDCFPCFLRQSIIALRLGTKDTVLQEKILKAVLHEVEQSD
                     TSRPPAYTTTFIHRKIRQLLRGDPFQQIKSEYNQIALALYPFLKKMVQECSDPLWTSS
                     RLAVAGNVIDFGIFTSVDIEGTVKRALHSPFAVDEYHAFKDALSDSEEVLYLLDNAGE
                     IVFDRILIETMVSHGKKVLAVVKGSPVLNDCTKADAIEAGLADVCEIIDNGSDAVGTI
                     IEWTSRDFQRKYAQMRFILSKGQGNFETVSADPKKTIVYLFQSKCEVVSRELGLSLGS
                     MILKRS"
     gene            complement(21331..21825)
                     /gene="purE"
                     /locus_tag="VMU21_09360"
     CDS             complement(21331..21825)
                     /gene="purE"
                     /locus_tag="VMU21_09360"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HUO77769.1"
                     /translation="MKSRVLVMMGSDSDLSVVEETGKILKELGISYEMTIASAHRTPE
                     LVQKLASEAEGKGIEVIIAAAGAAAHLAGVVASHTILPVIGVPLNSSPLQGLDSLLST
                     VQMPPGIPVATMAVGKAGAKNAAIFAAQVIGRKDKGIVKKLVAYRKRTALEIKKKADS
                     IKSL"
     gene            complement(21837..23111)
                     /gene="purD"
                     /locus_tag="VMU21_09365"
     CDS             complement(21837..23111)
                     /gene="purD"
                     /locus_tag="VMU21_09365"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HUO77770.1"
                     /translation="MKVLVIGGGGREHAIVWKLAQSRHVDKIYCSPGNAGIADLAECI
                     DIGTNNFDALVDFVKYEWIDLTIVGPEGPLSRGIVDAFEKQGMRILGPSEKAAQLESS
                     KVFAKDFMKRYGIPTGEYSVFSSYVHAENHVKMKGTPIVIKAEGLAAGKGVFIATDMD
                     EAINALKLIMRDRIFEDAGDRVVVEECLEGEEASFMAFCDGKTIKPMASSQDHKRVFD
                     GDKGLNTGGMGAYSPAPVITRELEGEIMEKVMWPTLRGLKAEGIKYKGILYAGLMIKD
                     NAPYVLEFNCRLGDPETQPVLSRMDTDLVDVAFAITDEKLSEVDIQWKPDSSVCVVIA
                     SGGYPGKYEKGKIIKGLESIKDEKDVYVFHAGTSFENDHVVTSGGRVLGVTGLGTDVG
                     AARDKVYGAIEKIRFEGMHYRKDIAERALKRK"
     gene            complement(23353..24906)
                     /gene="purH"
                     /locus_tag="VMU21_09370"
     CDS             complement(23353..24906)
                     /gene="purH"
                     /locus_tag="VMU21_09370"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HUO77771.1"
                     /translation="MIKRALMSVSNKKGIVDFAKELSGMGVEILSTGGTAKSLREAGV
                     SVVEVSEYTGFPEMLDGRLKTLHPKIHGGLLSRRSNPKDMKDIEEHDIKPIDMVVVNL
                     YPFEETISKPGVSFEEAIENIDIGGPTMLRAASKNFQDVTVVVDPDDYEAILREIKSS
                     KGALSKETKLNLAKKVFAHTARYDTLIADYLTGITEKEPSFPEFFTTTLRRVATLRYG
                     ENPAQKAAIYRERTKGLSLPDAKVLQGKEMSFNNYLDTHSALLLALEFNQKACAIIKH
                     NNPCGVAVGATAAEAYTKAAKTDPVSAFGGVVAFNTEVDEAAAKEMADLFLEVVIAPS
                     FTKGALEVFSHKPNVRLLALPDMLQPDKRRPGSWDIKRIAGGLLLQGWDYSTEDIMAL
                     KAITKRQPTKDEREALSFAWKVCKHVKSNAIVYAFKDRTAGIGIGQTKRVFSAQTGLL
                     NAGEPVKGSVAASDGFFPFRDGIDVLYEAGVTAVVQPGGSVKDEEVIKAADEYSMAMI
                     ITGVRHFRH"
     gene            complement(24975..25658)
                     /gene="radC"
                     /locus_tag="VMU21_09375"
     CDS             complement(24975..25658)
                     /gene="radC"
                     /locus_tag="VMU21_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HUO77772.1"
                     /translation="MANPKIKDWPENERPRERLIKYGTESLSDAQLLAIIIRTGGEGK
                     SVLALAMELLDKFGGLKNIDSASIVDLSLTKGLGKAKIAQLKAAFELGKRLMSEPIGN
                     TPVFSSSNLVYSYFASRFKNAKKETFLCALLDTKNRLVREVKISEGTLTNSLIHPREA
                     FKEAIRESAASVIFVHNHPSGDPGPSRDDIAVTERLKATGDIVGITVLDHVIIGDGTY
                     VSLKEKGIM"
     gene            25947..26108
                     /locus_tag="VMU21_09380"
     CDS             25947..26108
                     /locus_tag="VMU21_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77773.1"
                     /translation="MEKPFVGKLQNEVHYQCDTCYAIKVLKYVVMDHAGNILSICEEC
                     RDRILEESN"
     gene            26467..26703
                     /locus_tag="VMU21_09385"
     CDS             26467..26703
                     /locus_tag="VMU21_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77774.1"
                     /translation="MKESKGAIGISMHSDPDLNVMAAVAVLRDLQDEAIEPDTVIIAH
                     DTLILFAEDILQGSSCPRDEHTGGLLRAVANSAL"
BASE COUNT         6506 a         7058 c         6192 g         6967 t
ORIGIN      
        1 aaacatgata cccttcctct cgtgttcgga tggtcttgtc gaacctctga gagattggga
       61 tgctcttcaa atgttcttga accccccgcg tataggtgtg aagcgcttca tagaggttct
      121 ttcgtatgtg ggcaatgagg gcatcatcat atgcataggc aaagctcttt ctgaacctca
      181 tgccgcctgc tttctggacg tgagaaagaa ggacctgttg atcaggacaa aggactaaaa
      241 ggctttccgc aatggcgtgg atggtgctga agtattgatc agttgataca tcgacaccga
      301 atagagagta agtcttgcct gaaactgcct tgtatggtct atccactttt ctattataac
      361 ccgcaaaaag aacgctacgg aaggagcgaa gcattagctc caataggaaa acgatatcct
      421 tggcgaacaa gcagaagtgt ggattagcgg gtgttagccg ctccgattac attcactttg
      481 acaatcctac cctgataccc accagacgca ctttcttctt taactcaaac ttacccaggc
      541 acaagaacgc tgttttcctg atgagctcta cattgtctgt gggttctttg agtgtgcaag
      601 cccttgtctg tgtctgaaaa tcgctgaatc gtatctttac ggtaacatgc ttccccttgt
      661 attcccttcc cttgatgtca tcaacgacgt cttttactaa ttcggccaga ctcctggcta
      721 tggtctgcca gctgtttgta tctgcttgaa atgtagtctc cctgctcgca gattttggct
      781 cccaatcggt tatcagtgga gtgcggtcaa ttccaagaga tgagtcgtgg agatagttcc
      841 cgtatgacgg cccaaaatgt tcgataagcg tgtccagagg gattttcgcg aggtccccta
      901 cggtgtggat cgacatttct ttgagatagc cctctgtctt gggacccaca ccccaaagct
      961 tcctgaccgg gagaggccaa atacggcttt ctacatcatc agcagtcagt atcgtaagcc
     1021 catcaggttt ttgcaggtcc gatgctatct ttgcaagaag cttgtttgag gcaatgccta
     1081 tcgagcacgt gagaccagtg tgagctgtta tcttttcctt gatttccatt gcaaccagtt
     1141 cagatgtctt ttgaatctct gatatatcaa gaaaggcctc atcaatacct acatcttcca
     1201 ttaccgggct gactcccttg agcagttcct taagaattga tgagaccctt gcgtattctt
     1261 cgtaatcgac agggagaaag atggcatgtg ggcaaagctt atatgccgtt tgcaaaggca
     1321 tggcagaatg aatgccatac tttcgggcct catatgatgc cgttgatacg acccctcttt
     1381 ttgtgggatc tcctgatccc cctatgacca caggtttgcc acgaagttcg gggtgacgct
     1441 tctcttctac cgcagcaaaa aaagcatcca tatccatatg gagaattctc ctcattacag
     1501 attgctgccg ctcatgcaag gacaagatgc cttgagatcc tttgccattc cttatgcatt
     1561 gtaccaggaa atcgcctgtg gtggctttga gtaccaataa tcaattgaat ggcgtatcgt
     1621 gtgtggtgcg gactcgcagg tgcaacgaaa agagaggcat taaatgatgt agtcggaaaa
     1681 gaacataacg tagtcagata tgaaactcta ttttcattta aaaatgttca tcctcttctt
     1741 tacccgccct ttccgttttc tgaagccttt tcctgcgttc ccaggcactc atctcccagt
     1801 tgtctctcct gtgtgattcc ggaatccatg gaggaacagg cagttggaaa tgctgagaaa
     1861 gggcatttac ataaggttca tacatttggc gaagttcggt cagttctttc tccgcctcgt
     1921 ccccttcacg cagcggcagg ccggcagagg ccagggctgt gcgtaattca gtcagttctt
     1981 caggagtcag gcgatctcgc ttcgggttga aaggagcgcg tccaaaaatg agagagatgt
     2041 caacaactgc gtgtcgtgcc atcgcgaagg tgagcatcgc ctggcgtgca catgaaccct
     2101 ccatatacga gacgacgaag gcacttgtat cgaggatggc tgtgatggcg gcaagccacg
     2161 actggttatc atggtgagag cggaaatacg cgagcaccgg atacgagaga tggctttcga
     2221 gcagctctgc agcccagcgc tcccactcgc tgagatgctg gaggagcgtc tccatattgt
     2281 ctgaatcatg gcagcgtctc cgtaacatct ctgcagcggt aggcggcgat ccggcacgtg
     2341 catcgagaag tgagatgttt acctcacgcc tggagataga ttgattgagg gcaggaagat
     2401 acgcgaaaag aagcgcgaga aaaccaaatc caagtcctgc ttcaaccgcg gtaaacactc
     2461 gtgcaagcgg aacgacgggg gtaacgtctc caagcccgag ggtgaaaaaa gtggtagcgc
     2521 tcagatacag gtctgtactg aaaccggcgg tcccttcagg tgttctgatc ggagagccga
     2581 gtgaccagtg caggagcgca aaaccgacaa tcaatccata ggcccagata ctgagcagga
     2641 gtagcagaga aagcggtccg aaataactga gaaaggtttc ctgacgcccg cccttggata
     2701 tcgaacggaa aactgccgac caaattcgcc aggcgtttct gtagaagaaa cgggtgaaac
     2761 ggtatctgcg ggtcacacga cggggcagca atatcacctc gaaggcttcc cagagaacaa
     2821 gaacaataag ggcgatagcg gggacggaca agagaatcgg caccttcacc tcattacgta
     2881 ttaagattaa tcatggatta atcagcatga acatatccca atttcctttc cccaaagtct
     2941 tccctgcgat cctattctgc ttctccctaa ttgaataatt atatatactt gtatcagtat
     3001 ttctcaataa tttcaataca ttttgagtga gggtcaggag ataaaaacct caataacaaa
     3061 ccgcttataa acatcctttg ttgataacat aggcatagcg atggttgagt tcctgtgata
     3121 tgccgaagct cagaactttg gtgccaagag caacgcgtct gttgtataat aaacaaaatg
     3181 gacgactctg aacttaaaga caccgttgag cttctctgtc ccgcatgcgg ggcagtgctt
     3241 accttggagg tgcgcactga ggatgggtgg aactgccagt gtggtgattt tatccctgac
     3301 ggcctggcaa taaacccttc tcagggctgt tcctgccagc acaagcagaa ttgttcgtgg
     3361 agatagaagc gctgcggtta aggggcgatt tcactcaaag gcatctttga tgtgttcgat
     3421 tttgtggatg tctccgtcag tctctatgct cagaacatca aaccttgagg ctacttcacg
     3481 ccgggagtgt ttgagataac tgagggccac cttcctgagc ttttctcgtt ttctatgggt
     3541 tacggcctca aagggatggc cgaatgaatc atctgtcctt gtcttcactt ctacaaacac
     3601 aagggtctct ccgtcctctg cgatgatatc catctctccc acaggcgact tgaagtttct
     3661 cgaaacaatt ttgtatccct tctttttcag gaatctcacc gcgagttcct cgccctcaat
     3721 gcccagctgc ctcatcgcct tcaagctttc ccaggagttt tggtatgccc tcgatgtcct
     3781 taatgtcctt ctggcagagt atcttgagaa tctccctgaa cgctgaaaat tcctcccact
     3841 gtcctctcag gtgcttctgg atcgtcttgc tcaagtgttc gcctttttcg tcccaatcca
     3901 ggaggatgac caccctttca aacctctcag aaatatcctc ggagaattcg taaagattgt
     3961 tcccgctgtg gaggacaatg acctcacccg caaggccgag ccttctcagg gccatggcat
     4021 ccctcttgcc ctcaacgatc acagggaatt tcttgttcac ctcgtagagt gcctccagga
     4081 cctccctcag tcttgacgcc ctttccatgt ctccgaaggt aggtccctgt gcgtgaacct
     4141 tgaatggccc gcggtctttg tggggttgcc tgctgtggga cttgatcatt gctgcctcaa
     4201 gacaaacctc tcattcaaga atttggccat aggcctttgc agctagagcg cattagtgat
     4261 ctttataatc tcctggagtt tttgggtggc cttgcccgaa tcaattacct cggatgccat
     4321 ggggagcgct tcctcaaaac tccttgcctt gtctgcaacc acaagggctg ccgcggtgtt
     4381 catgagcacc acatcccttt tcggtccctt ctcgccattg aaaagggagt ggagtatcct
     4441 cgcattctca tgtttgtcgc cccctgaaag gctttgcaca tctgccttac ggaaaccgaa
     4501 atcttcgggg gtgatataaa aggtctcaac cctccccgct ttccatctcg acacctttgt
     4561 gccatccgaa atagtgatct catccagacc gtcttccccg tgaaccacca tgatatcctc
     4621 tgccccaagg ttgccgagaa ccgctgcgag ggtatcggta agcctgtcgg caaacacacc
     4681 cagaatctgc ctctttgccc ctgcagggtt tgtgaggggg ccgagaatgt tgaatatagt
     4741 cctgatccct atgtccttcc ttggtcccac ggcatatttc atagcaggat gaaaaagagg
     4801 ggcaaagaga aaaccaaacc cagtttcgta gagggaccgc tcaaccttgt caggaggaag
     4861 atcgattctc acgccgaggg cctccagcac atccgcactt ccggactggc tcgatacaga
     4921 gcggtttccg tgttttgcca cagggacgcc ggcagcagca gttaccaaag cggctgcagt
     4981 ggagatgttg aatgtatggg ccatgtcgcc gcccgttccg caggtatcga ggactccctc
     5041 cggagccttt atagttgccg ccttctccct catgaccctc gcagcccctg taatttcttc
     5101 cactgtctcg cctttaatgc ggagggcagt cagaaatgcg gccacctgcg cctcagtggc
     5161 cctcccctcc attatctcct tcatgcactc ggccatctct gcctcagaga gatggatgga
     5221 ctggacaagg aggtgaatgg cctctttgat caccttcttt tccccagctt caggaaattt
     5281 ttcagcaggg ctttcccttc ggtggtgaga atggattccg gatgaaactg gacaccctcc
     5341 acgatgaact ccctgtggcg tactcccatg atctcatccc tgtctgtcca tgcggtgatc
     5401 tcgaggcatt ggggaagggt ctctctccgg atgagaagag agtgatatct cgtcgcctcg
     5461 aagggattgg aaagtccctt aaaaattgtt ctgccgtcgt gatggatcgt ggaggtcttc
     5521 ccgtgcataa gcctgggtgc acgtatgatc tctccgccaa aggccgctcc gatggattga
     5581 tgaccgagac atactccaag gaggggaatc ttccctgcaa acttgcatat gacttcaaca
     5641 gaaattccgg cctcattagg ggtacagggg cccggagata ttacgattct ctcagggccc
     5701 agttcccgaa tctcctgtat ggtgatcgca tcatttctga atactctgat ctcctctcca
     5761 agctctccga gatactggac aaggttgtac gtgaaagagt cataattatc gatcatcaaa
     5821 agcatttatt caaagtcctt ttctgccatg tctactgcct tcatcatcgc tatggcttta
     5881 ttcacggttt ccccatattc cccctctggg tccgaatccg caacaatccc tgctcctgac
     5941 tgaacataga ccttctgatc cttgataatg agcgttcgta tggtaatgca ggtatccatg
     6001 tttccggaat agctgaaata gcccaccgca cctgcataag gccccctcct ggtaggttca
     6061 agctcctcta tgatctccat tgacatcacc ttcggagcgc cgctcactgt accggcagga
     6121 aaagtcgccc tgagcacatc aaaggcatca agcccctccc ttaattcccc ctctacatga
     6181 gagacgagat gcattacgtg gctatatctc tccactgaca tcaactcggt cacctttact
     6241 gagcctgtcc tggcgactct tcccacgtcg ttcctgccga ggtcaacaag catgatatgc
     6301 tcagcaatct ctttggggtc ttttttcagt tcctcctcca actccttatc ctctgactcc
     6361 gtctctccgc gcctcctcgt gcctgcaatg ggtctcagga caaccgtctt cccctccagc
     6421 ctcgccagga tctcagggga tgaaccaacg atccttgcct ctcccgtgtc gaggtaatac
     6481 atatagggtg acggatttat cacccggaga gccctgtata cgttaaacgg gtccaccgac
     6541 aattccctct caaatctctg ggaaagcact acctgcacca catcacccgc catcacaaat
     6601 tcctttgcct tcgaaactgc ctccttgaac gcctccctgg taccaaagga agaggtgaaa
     6661 gaaccgttca tcctcttttt cccctccctg actccctgag ccctgcctgc cactgcgggt
     6721 gggggaacgg tgactgatgt ctggttaagc ctttcgatga tctctctaat cttgtcctca
     6781 gcctcgtggt aggcctcatc cggttttttc ccctcaatat gtgcattcga gacaaccttt
     6841 attttctgtt ggagactgtc gaaaatgagc atggtgtcag tgagcataaa aaacatgtcg
     6901 ggaagtccaa ggccttctct ggacctgtcc ggtatctttt caaaaaatct caccatatcg
     6961 tagcccatat atccgaccag ccccccgaag aacctcggga ttcccctgac ttctacaggg
     7021 acgtaccgtg agacctcctt ctttaagacc tccaggggat catctgaatc gaacaccata
     7081 gggggcttgc cctcttcccg tatctcgacc cttctccccc acgccataat caccttagac
     7141 ggtcttgaac cgatgaagga gtaccttgcc catttctccc cgcccacaac gctctccagg
     7201 agaaaggaag gtgtaccccc cagtttgaga aaggcagtga caggagtatc catatcagcg
     7261 aggatttccc tgtaaacggg aataaggttc ccctttgcac agagggcctt gaattcgtta
     7321 agggcaggat aaagcatatt gacaaaaccc gcaatttttc attagtattc tatatccgct
     7381 cattatatca cagtaagcga gaagagtaaa aaaacactgc gggtgtagct cagctggtag
     7441 agcacaacct tgccaaggtt ggggtcgcgg gttcgaatcc cgtcgcccgc tccataaaga
     7501 acggtgaatt gtggtgagtg aatggctgtc agcatcaaag accaagtcat tgtccactga
     7561 tgcttttgtg gagagttgtt attaaaagga ttttagcaag ttactgttca ctatttactg
     7621 atcacggtag ttgatggcgg cgtacccaag tggctaaggg agaggtctgc aaaaccttta
     7681 tgcagcggtt caaatccgct cgccgcctcc agttcccgaa aattccttat cctccctctc
     7741 ccattcgaga ataaggggac ccatctcgtc gtccttaaga tactcctttt caaggccctc
     7801 gatatagaac cggtcaagcc cgctgttgtt gagaaagtct tccctgagtg ccatatctct
     7861 gttttttgta atcgcgggca ggaggtgatt caggtagtat gactctttct tgagatccag
     7921 gagcaccctg ttgaacaact gttcgtacag cttcaggttc gtccccttcc cgacaagttc
     7981 ggcttcgatt tcaccgcgta tcctcatcat ctccttcttc atgttcagcg aggtatacaa
     8041 ctcatgcatc ctctccttca gggcactacg cacatactta tagatcttct cctcttccgc
     8101 aataccctcc agcgccacta tcagatccct taacgaggcg tctcctctga gaactttctc
     8161 gatgtgctca tgcaatactt tgatcttctt cttgccaaag cttgtgatgt atctgtttga
     8221 gaggaggtca ctaatgaaaa tctccttaaa gagattcttg tccagagcat caaactcttt
     8281 cttgttgccc acgaggcttc tcagggaatc ttccgagagc tccgggtgtt ccataaacgc
     8341 gatcctgctg agagacgaat gcacattgtc gtaccggtca aagtaggtga tgatggcgct
     8401 gagttcctca aagatgctga aatcattctt ttcgcgggca aattcatcgc atgactttcc
     8461 aatatccagg agaacccgct caaaccccat atcgctctgg gaatgggagc tatgtttagc
     8521 ccagatcaac cgcgcgatat cttctttgat aatgtgcctc ttgagtgaag ggcttttgaa
     8581 aaaaagattc tcaagaatgg ccctggactc cttgagatac tcaggttcct caatggcctg
     8641 cacatccttg tccttgagga gaaactcatc gagggtgtca aaaagaatgc cggggatatt
     8701 attccgtatg cttaatgtgc gcaaccggtg gagttttgca atctccgaat tctggatcgt
     8761 ctccttatac gcaatatcga ggaggatatc tctgtattca tccacgatgt tcctgttttc
     8821 gtgatggcgg tacatactgt cgatcttcat tctctcctgc tggtagtcat ctatctgata
     8881 gtttgcggcg attccgttga gacgcgcctc ttcttcgggc gtaagcgccc ttttaaggga
     8941 ataggctgca tggaaggcct tatagaattc cagattttca gactggacaa gtttgaatat
     9001 gaacaccgtc gaacggggct cgctgagctc ttttacaaat ccacttaaca gctcgccgtc
     9061 gttgtcctca gcaacaagat ccgttctttt caggaacttt cccaaagatc tcacaatgtg
     9121 ccgctgtctc ttcctgggtt cattcctgtc gtcagaggag atgaagaaat agtagttttt
     9181 taccgcatga ccgaggagaa aaagtctttg aaaagactct gagccggtgg tatcattggt
     9241 aaatataagc ctctcatcct tgtcaaggaa ggccccaaac ataacgaggc gattcagcac
     9301 ttcattctta acgaggtcct tgaatggttt ttcgacgccg aacatgtatt cgcaaaaact
     9361 tccacccgta cctttgtaat gaacaccttc aggggccaac gtgagctcat tgccccggga
     9421 aaaaaacctt atgcgcggac catcttcctc ataaaaatag gtgtttcccg cgttcgctcc
     9481 tcccataaaa acaaaaaaat ccactggacc gatactgccg tggagcatga aatccttgac
     9541 catataagaa tattaccaca tacagaacga aattcggaac tgttctcatg ggccagaagg
     9601 aatcaggaac acatgtccta cgcaagaaat tccccgacat gcgaaagagg aaatatcaga
     9661 cggcaagggg caaaatcggg tgaacgatta ggaggtcact caaagagccg ttctttttga
     9721 tgaactctgc ttaaaaaaaa cgctgctcag ggtctggtac ggtcttcaga gcaaaccctc
     9781 accaatgtct ttacattacc cctcggatta aagtcccccg tgacctcaag aaaccgtggt
     9841 cttactactt tctccaattc cgagtagatc ctgttggtaa cctcctcatg ggatgtatgg
     9901 acatcccgaa aggaattcaa atagagtttc agcgctttga gttccacaat tttcttgtca
     9961 ggcacatagg ttatggtgat ggaggcgaaa tcaggatacc ctgaacgggg acagaggcag
    10021 gtaaattccg gaaaactgat ggtgatctca taatccctct gcgcagaggg gttatcccat
    10081 gtctgcaatt tcgcctttct tatggctttt tcaccgtacc gaagcatgaa aaaatctaac
    10141 attttatggg aaattttgta aagaaatgca ttccccttga caagagagca tttttcggct
    10201 attaatatac catatggtta ttttgtggtc ttttgtcgtg gtatgcctgt ttctgctttc
    10261 tcccctcaat ctttctgctt ctgaaagcgt ttctgacagt ggaatccaac cagcctcagt
    10321 cggagagacg caaagcaatc cctctcaaga gaaggaactg cccccgatgt atgtggaaaa
    10381 agaaagtgcc acaagcgcac aatatgttcc gagtccggta gcgacaaagg caatcgatag
    10441 aaatatatcc ctcttcaccg agaggataaa ggagagattc tctctttatt tagagagatc
    10501 cgggaaatac ctcgcgttga tgaaagagat attgaaggcg aagaatatgc cggaggatat
    10561 cgcctttctt cccctcatag agagcggctt taatcccaac gcgtattctg tagccagggc
    10621 ggtaggtccg tggcagttca ttgcatccac tgcgaggaac tacggactta ccatcgattg
    10681 gtggagggat gagaggaaag atccggtaaa gtcaactgag gcggccgcga attatcttaa
    10741 ggatctttac gagatgttcg gttcttggaa tcttgccatg gccgcatata atgcgggcga
    10801 aggcaaaatt ctcagggcaa taaaccgaac aaagagcgat aattactggg cattgctcaa
    10861 tacccggcat atcaagaaag aaacaaagga atatgtgccg cgcttcattg cagcaaccct
    10921 catcgcgacc aacccccatg attacggttt tgctgacctg gactatctcc cccccctccg
    10981 gtacgacgaa gtggttctgg atagacccgt tgacctggat attgctgcgc ggtgtgccga
    11041 aacttccctt gacatgatca gggagcttaa tcccgaaatc aggagatgga gcacgcccgc
    11101 taatgtgtct tcttatgcgc tgaggatacc gtttggtaaa acggaaatct ttaccgaaaa
    11161 cctttcagag attcccgaag aagagcggtt ctccatttcg acctataccg tgaaaaaggg
    11221 tgatacaatc aagacgattt cgaccaagac aggaatacct atcaatgtga ttcaggaact
    11281 caatgacaac ggcaatggcc tgaaaaccct taaggccgga aaaacgatct ccctgcctcc
    11341 aaaagaaaaa ctttgctttg accgtgacga cagagagggg aaaagatcgg caaagaacag
    11401 aagatctaaa ggcaggaagc aggcgaacaa gaagattgtt gtcacctcgc taagcaaaaa
    11461 gcaggcatat atagatgact aggatagtga gcccgtaaga attctacgac ttttccggcg
    11521 ttttctttcc agaaagtttt atccgtgcct cttccgcaaa tggtgacgca ggaaagttct
    11581 tcgtaatctc ctcatacttt tgtgccgcct cagcggtctg ccccattgat tcaagaatcc
    11641 gcccggattc tataagagca aggtctttga atgacgcggt cttataattg gacagggtat
    11701 ttaaaagctt aagggcagcc tcccggtcac ctttcctgag attaatcacc gccattttat
    11761 aataggtaag aggaacgaac cgctcgtcat cagggaaccg ctcattaagt tccttgaggg
    11821 ccttcagtgc ctcatcatct ttgcccattt catagtagca attggcaaca taaaagagtg
    11881 aatagggacc cttcctgagt tcgtatgcct ttctgaattt ctccagtgca gcctgataac
    11941 gcccctcctt ctgaaggggt tgtttctgga acatgccata gtacagctta tacccctcat
    12001 attccagggc ccctgccctc tcccttgcgt tggaacggta catgaagaac cctgcgagcg
    12061 ccagacagag tataacacag gaaacgacga ctaaaaaaaa gagcttctgt ctctctgcaa
    12121 tcatctctct tacacggaag atgatactca tcatcccttc ctctgttttg accggctttg
    12181 tgactttctt tttaatagcc tttggcatta atccctcctg agcgcttttt ccacaagcag
    12241 tcttgaattc tcgaagaccg attcgatgcg gctttcttcc actcctgcac cgaggagtat
    12301 cctccgggcg gtctcctcgg tgataaggtc acccggtcca tgagtatcgg tattgaggca
    12361 aaggggaaca ccgaacttaa tagcctcttt tgcaacgtaa ccgttggcaa gagagtgccc
    12421 tttccgtgca gtaatctcga ggattacccc tttatccttt gcataaagga catcctcagt
    12481 ggtgataagc ccgggatggg agaggatatc ggctccggct tcgatggcgg ctttgttcgt
    12541 gccctccgca acaggctctg taatggtctc accatgaaca aggacaaccc tcgcaccaag
    12601 ggaccgggcc tctttcacaa gctcatggat gagtcgcggc ggcgcgtggg ttatctcagc
    12661 accagggagc acctcaatgg agacgacctc cctcaacttc ttgagtgcct tgacaattct
    12721 cggaaccaca aaatcaatgg tggaaggatc cacatgatcc gtgagcgcca ttgcattata
    12781 gcccattgcc tcagcccttc ttacaagttc atacggcaaa agctcgccat cgctgaatag
    12841 agtatgacaa tgcagatcga tcatcataaa aagataccgc aaacaggcat gaaatgtaaa
    12901 gacagaccaa ctgcattgga cagatcagcg tacgtgtgaa ggtaaaccat tcttcacata
    12961 ccctattgac actccctttc acctgcccct atacttcaag cgtatatgac ggaatggaga
    13021 tgtattgatt ccggaccttg ttccgcctct tataacatgg ctctggacga ggctatctcg
    13081 cttagtgtca gaaaagggtc atcccctcca acgctgagac tatacgggtg ggagaggcca
    13141 tccgtgagca tcggcttgtt tcaaagaata agtgacattg accttcaata ctgtatcaag
    13201 aataacctgc ccgtggtgag aagacccacg ggaggaaggg ccatcctaca tggagacgag
    13261 ctcacctaca gtttttcctc tggaaacgaa ggattttttt caggggggct tctggatacc
    13321 taccgacagc tcggcagtgc actaaaatct gcacttgaca tgctcggact ggatatcacc
    13381 ataaagaggg agcgggcaga gggaaggact cttacaagga gcccactttg cttcaggtcc
    13441 gtatcatacg gcgaactcag ctgggagggg aaaaaactag tcggttcagc ccagaaacgc
    13501 tggaaggacg gttttctgca gcagggttct cttccctatc tcatagatga agaaaataca
    13561 agaaaggtct tcagcattcc ttcctcttcg gacataaaaa aaggaatgat cggattgagg
    13621 gaggcggttc caggcatcga gcctagacag attaaggagg ccattcgggc ctcctttgag
    13681 agaagcttcc gaatcagcct tctttattct ggtctttctc ctgaggaatt tcaccttgcc
    13741 cgggagcttc aggttcagaa gtatgtatct ccggagtaca ctcttcagcg gtagtcaaag
    13801 gaagagaggg cccggggggt tccttcaaga ctcctgttgc gcgctggtag taccagaaga
    13861 cagccgcaat catcacaagc cccagatagc tctgcataat atagagacaa aagtggtaca
    13921 caagagctat aagaggtccg atgaatggta cagagcctat ggggtaactc agaaatataa
    13981 tgagaaagct gatggcgatg tatccgcaga aaatcacgca atagagatat agcccttcgg
    14041 gacttctgta gagataacgt atcgactctt taagggattg aaaagggccc atccccctta
    14101 aggtaatgat ggcggcgcca taaatggtaa ttgagagcgt gagaaagata agaaccatcc
    14161 ccaccacaaa gaggatgagg gaaaagaata tacccaggaa aagtgccaat gttgcctctt
    14221 gttctctggc gaccgacaca atggccgcaa tggcacctcc gaagaggccc aaaataaagg
    14281 cgaccgccac aaatagcaaa cccaccattg tggtaaatcc cgtgagagga aaaaacctct
    14341 gccttccctc ggctatgaaa accttcatgc ggaacttctc ctgctcaccc ttgagccatc
    14401 ggccgatgac ccccatggat ccgcccaaca ggaagatccc tatcaccagc acaacaagga
    14461 catagaggag gagacttgcc aacacaagaa ccactaaggc aaagtacttt gtaagtatct
    14521 cggccggttc cttgaaggtt ctcagcgcat cttgaatacg agagagttca gtgaggtcca
    14581 gtccgaatat gataaaggct atggcaagag gcacacccac aacgacaaaa aaactcatga
    14641 aactcgcacc catcgcacat acctgaatga gcacgagctg ccagttttta ttgactatcc
    14701 tgaatccgct ttttattgcc tcggtatagt tcatcctctg tcctgaccga tctccccgct
    14761 gcgtgcggcg attctcccgg aatacttcta ttttagtata acccctcaaa gaaatgaagg
    14821 atgacacacg ttacgaagca ccctttgccc tctcgataaa gagccgcatc tttccttggt
    14881 ctttcttccc ttttctttcc tcaaccccgc tgcttacatc cacaccatac ggcttcaccc
    14941 atcgcaccgc cttgtcgaca ttgtccggat taagcccgcc tgcaagaatt atcttcccaa
    15001 attgctttgc atccaacgcg atatcccagt tgaatatttg ccccgtaccc ccgaaagact
    15061 cctgcgtata ggtgtccagg agaaatgccg agacacggta gttctttaac ggctcaaggt
    15121 cactcagttc ctttacccgt atcgccttta tgactcttct gccgagcaca catgcctcgg
    15181 gtaattcgtg accatgcagt tgtatgacgt caagagacgc atggtgcata atggcctcca
    15241 cctccccgag tggcatgtca acaaacactc ccaccgtagt gatgaaagcg ggaagtctgc
    15301 gtacgatctc cctcgcctgg tcaggggaaa catggcgcgg gctttccttg tgaaagacaa
    15361 acccaagcgc gtcaaccccc acatctgatg cagcgagggc gtcgtccaca ttggtgatgc
    15421 cgcatatctt taccttgact gtcacccttc acccctgtac agagggataa ccgaggtctt
    15481 ttctccgtta cccgataaca ggcaactttg ccagtgactc ctttatcttg tcatccggat
    15541 atgcgtaatc ctccagtttt cccgaaagat aatctgcgta cgcagtgaga tcgaggtagc
    15601 catgaccgct gaggttgaaa aatatggtcc tctgtttgcc ctcttccttg catcgcatgg
    15661 cctcgtcaat ggtcgccctt atcgcatggg cggtctcagg ggcaggaatt atcccctccg
    15721 cctttgcaaa ggtgactgcc gcctcaaata ccgcggtctg cccgtaggca accgcatcga
    15781 tcaaccggtc atggtagagc tggcacaaaa gaggcgcatc cgcgtgatag cgcaaccccc
    15841 cggcatggat tcccggcggc ataaaatcgt gccccaaggt gtacatcatc agcaggggag
    15901 tgagacctgc cgtatcgccg aaatcatacc tgaattcacc cttcgtaagc gttggacacg
    15961 agatgggttc cacggcaatg accctcaggt ccttcccgtg aatcttgtca aggaggaagg
    16021 ggaaactcgc ccctccaaga ttgcttcccc cgccacagca gccgatgatc acatcaggat
    16081 agtcccccac aagtgcaaac tgcttttttg tttccaggcc gaccaccgtc tggtggagaa
    16141 gcacatggtt taacaccgaa ccaagggaat agttggtgtc ggaatgggtt gccgcatcct
    16201 ctaccgcttc tgatatggcg ataccgaggc ttccctgact ctcagggtcc aactcaagga
    16261 tttttcttcc cgagttggtc accgtggaag ggctggggtg cacggtcgcc ccccaggtct
    16321 ccatcgcaat tctcctgtag ggtttctggt cgtagctcac tttcaccatg tataccgtca
    16381 cctcaaggcc aaagaaactc cccgcgaggg ccatggaaga accccactgt cctgctcctg
    16441 tctcggtggc aatcctcttg atcccggctt tcttattata atatgcctgg gggattgaag
    16501 tattcggctt gtgactgccc gcaggactga ccccctcgta cttataataa atctttgcag
    16561 gggtgccaag tgccttctcc agcctgtgcg ccctgtagag aggcgatggc ctccagaggg
    16621 agtagatgtc aagtacctct tcaggaatgt cgatccaccg ctgggtggag acctcctgct
    16681 caatgagtgc catggggaat atggcggaaa ggtcgtcagg ccccaccggc ttaagagtgc
    16741 cggggtgaag gggcggctta gggagattcg gcatgtccgc catgatgtta taccactgct
    16801 tcgggatctc tctgtcaggg agcacaatct tcgtttcctt catctcatcc tccttaccat
    16861 gaatgtaatt cttatgcccg cgcaaagcgg gccattctca ttgttccgtt aagcccctca
    16921 gttcgtcgag tttttttccg atgtcccggg atgtcataag agatgtaccg atgagcaccg
    16981 catcaacacc gcttgtatcc aagcttagca tatcattcct cgtctttatt ccgctctcgc
    17041 tcaccacaat cctgccggac gggatctcct ttttaaggga agacgtggta tgtaggtcca
    17101 cggagagggt gttaaggtcc ctattgttta tgcctatgat gtccgcatcc agacgcatcg
    17161 ccttctccag ctcatcaagg ctgtggacct caaaaagaac gaaaagtcca agttctgcac
    17221 agagatggaa atattcttca gcctgggaca tcccgagcaa agacgcgatg agcagaagcg
    17281 catccgcccc gtataccctc gattcgtaga tctggtactc atcaattatg aaatcttttc
    17341 tcagtatagg tcttgtgacc gcccgtttta ccacaggcac attcctgata ttacccagga
    17401 aaaagtcctc ttcagtcagg acagagattg cgtccaccgc cttttcctca tagatatggg
    17461 cgatgcctgc gaggttgaaa tcttctctta tgatgcctcc tgagggcgaa gccttcttta
    17521 tctctgcgat aaaccggata tgtccctcat cccttctgat ggccgtgcgg aaatcacggg
    17581 gcctctcggt gtcaggaatc cgtgctttca gatcgcgcag gggagccgcg ccttttgcgt
    17641 gaatcagcct ttctctcttt ttctcggtta tcctattgag aatggacacc tatggatttt
    17701 aaggtaaata cggagatttt tacaacacta attatcggtc cttggtgata ctatctgccg
    17761 gcaatccccg ggggtagtgg tatcttttat gatataattg cccttgatgg acacaaaggg
    17821 gacgatcgca agatcagcag gaatcatatc cttagctacc ttcatcagca gggtcctcgg
    17881 ctatgtaaag gacatgatcc tcgcccgcta ctttggcgca acgggcatct ctgatacctt
    17941 ctttgtcgct ttcagaatac ccaatctcct gagggaactc tttgccgaag gatcaatgtc
    18001 atctgcattc ataccggttc tcacagaata ccagacgaat cagggtgctg gggaggcgaa
    18061 acggcttgtg aggatcacct tcaccttcat gctgatatgt gcgggattat tttgtgtggc
    18121 aggcgtcatt tttgcaccgc aactcgttgc cctcattgct cctgggttct taaacaatcc
    18181 cggaaaattt tcccagaccg tacttctcac caggataatg tttccctttc ttctttttat
    18241 cagccttgcc gcacttgtca tgggcgcact taacacacga agagtgtttt ttgtgcctgc
    18301 cctggcacct gccatgctca atgtgaccat tattcttgtg gtattgaccc tggttcccct
    18361 tctgcagcaa cccatccttg cggtggcggt gggggtcaca ctgggcggtc tcgtccagtg
    18421 cgggtttcaa cttcccgtgt tctttcaaag gggatacgac ctccgcaccg attatcggtt
    18481 cagacaccct gggctgaaaa agatgtccat cctcatgatg cccgctacca tgggaatggc
    18541 ggttgcccag gtgaacatct tcgtcagtac catccttgcc tcctatcttt cccagggcag
    18601 tatcacctat ctctattatt ccatgaggct gattcagttt cccataggca tttttggtgt
    18661 ggcgatgggc atggcggtac ttcccacctt atccgagcat gcagtgagag gggagatgga
    18721 caagctcagg gaagacttct ccttcgcgct gcggctcctc tttttcatca ccgttcctgc
    18781 catggtagga ctgattgcat tgagagtgcc ggtggtaaac atcctcttcc agcgtggcat
    18841 gttcgattac ggggccacgg tgggaaccgc ggatgcgctt ctcttttatg ccacagggat
    18901 atgggccatt gtgggagtga gaattgttac cgccaccttc tattccttga acgatacccg
    18961 gacacccgta aaagtcgctg ctgccgcagt ggtgattaac atcatcatga gcctgctcct
    19021 catgaatccc ctcaagcatt ccggtctcgc attcgccaat gccattgcgt cgagtgtcaa
    19081 ctttatcctc ctcttttatt ttttgcggcg ccgcctcggc cgtatcgatg cgaaaaggat
    19141 tctgagatcc ttcacaaaga tttgcgttgc gtcgattatc atgggcgtgc tgggatggcg
    19201 tgtccttcac ggtgagatgt ggatggcgtc cgggagagtg gtggaaaagg ccctctatct
    19261 ttccggcacc attgtggtat gcgtaggtat ctacctgacc atcaatttct tgctcaaagg
    19321 cgatgaattg aattacctgt atgaccgcat taaagaccgg ctgaggagga gtgccgcatg
    19381 attgtcaggt caattcccct ggggccgctg gaagcaaact gcttcatagt agctgacgag
    19441 cattcaagaa aagcaatggt catcgaccct ggggacgagc ctgacacaat catggacgtt
    19501 ctgaagacgg agaacctttc ccttgagtac atcatatgca cccatgcgca ttttgatcat
    19561 gtgggtgcag ttcccgaaat taaggaacag acgggtgcaa ccatcgtgat tcataaggac
    19621 gaacaggaga tttaccatgg tgcaagggac atggcagcat tctggggata tactcttgac
    19681 ccgcttccag aacctgacct ccttgcagaa gatggccatg aaataacaat gggaagtctc
    19741 acctttacgg tgctccatac ccccggtcat agtcctggcg gtatctgtct tttcggcgag
    19801 gggatagtga tcacagggga tacgctcttt gccggctctg tgggaaggac agacttccat
    19861 ggaggagata cccggaagct gaagcagtcc ttccaaaggc tgatgtctct tccccctgaa
    19921 acggtggtgc tcgcaggaca cgggccgaac tcaacaatag ggagggagcg ctcagagaat
    19981 tttttcccga aattatttac ttgacaaaat cattgtgaac tggcatagtt acgtatcatg
    20041 atgtgagcat cgctgcatca ccgggtcccc caaaaatcat tgattcttga ggtgccttta
    20101 cccaggacca aaggtcttgg gattttattt ttaggaggaa ggatgggaaa caaactttat
    20161 gtaggcaaca tttcattcca ggcatctgag gaggacctga aggatctctt ctcaaaggca
    20221 ggagaggttc tctcggcgaa gcttatcact gatgctgcca cgggaagggc acgggggttc
    20281 ggattcattg agatgagttc tgatgcagag gcccaaaagg cgatctccac gctgaacggt
    20341 acgagtttca tggataggaa cattatcgtc agtgaagcga aaccacagga acgaagagag
    20401 aggggcacaa gagatcgcgg aggttttgga agcagaagac cggggggaag aagatagttt
    20461 aggttccaag atctcacgac ataggtcagg atctcttcag aatcatagag cccagggaca
    20521 ggcccagttc tcttgaaacc acttcgcatt tggactgaaa gaggtacact attgtctttt
    20581 taggatctgc tgacactgtc tcaaaatttc cctgcccctt gctgagaatg aaccgcatct
    20641 gcgcatactt cctctgaaaa tctcgggaag tccattcgat aattgtgccc actgcatcgg
    20701 aaccattgtc gattatctca cagacatccg ctaatcctgc ctcaatggca tcagcctttg
    20761 tgcagtcgtt cagcacagga gaacccttta ccactgcaag taccttcttg ccatgagaga
    20821 ccatggtctc tatgagaatc ctgtcaaata caatctctcc ggcattatcc aggagataga
    20881 gcacctcttc tgaatccgag agtgcatcct taaacgcgtg gtactcatcc actgcaaagg
    20941 gactgtggag agcccttttc accgtgccct caatatcaac agaggtgaat attccgaaat
    21001 cgatcacatt tcctgctacg gcaagccttg aggaggtcca aagtggatca ctgcattcct
    21061 gaaccatttt ctttaagaac ggatagaggg caagggcgat ttggttatat tctgacttta
    21121 tctgctggaa ggggtcccct cggagcagtt gccttatttt cctgtggata aatgtggtgg
    21181 tataggcagg aggcctagat gtatcacttt gttcgacctc gtggaggacg gccttcagta
    21241 tcttctcctg aagcaccgtg tccttcgttc caagacgcag ggcgatgatc gattgtctca
    21301 ggaaacaagg gaaacagtca agatggactc tcacaaactc tttatggaat cggccttttt
    21361 cttgatttcg agggctgtcc gcttcctgta cgccactagt tttttcacga tgcccttgtc
    21421 tttcctgcct ataacctgcg ccgcgaatat ggcagcgttc tttgctccgg ccttccctac
    21481 tgccatggtc gccacaggaa taccaggcgg catctggacc gtactgagaa gggaatcaag
    21541 cccctggagg ggagatgaat tgagggggac cccaatcaca ggaaggatag tatgtgaggc
    21601 gacaaccccc gcaagatgcg ctgccgcacc ggcagccgcg atgattacct ctatgccctt
    21661 cccttcagcc tcactcgcga gtttttggac cagctcaggt gtccggtggg cagaggcgat
    21721 ggtcatctca taggagatcc ccagctcttt cagtatcttt cccgtttctt ctactacaga
    21781 cagatcagaa tcacttccca tcattaccaa gactctcgat ttcatactcc tcccccttac
    21841 ttccttttca gtgccctttc cgcaatgtcc ttccggtaat gcattccctc aaagcgaatc
    21901 ttctcaatcg ccccatatac cttatcccta gccgcaccta catcagttcc cagaccggtc
    21961 acccccagaa cccttccccc cgaggtgacc acgtggtcat tttcaaagga ggtccctgcg
    22021 tgaaatacat atacatcctt ttcatctttt atgctctcta gtcccttaat aatcttccct
    22081 ttctcatact tccccggata tcctcctgat gcaatcacca cacagacaga ggaatcaggt
    22141 ttccattgaa tatccacctc cgagagcttc tcatcagtaa tggcaaaggc aacatccaca
    22201 aggtctgtat ccattctcga aagaaccggc tgggtttcgg gatcgcccaa ccggcaatta
    22261 aactcaagga cataaggggc gttatctttt atcataaggc ctgcatagag tattccctta
    22321 tattttattc cctcggcctt cagccctctt aacgtgggcc acatgacctt ctccatgatc
    22381 tccccttcca actccctcgt aataacaggg gcaggactat atgcacccat ccctcctgtg
    22441 ttcaggcctt tgtcgccatc gaacacccgt ttatgatcct gggaactcgc catgggttta
    22501 atcgtctttc cgtcgcagaa tgccataaag gatgcctcct ctccctcgag acattcctcc
    22561 accaccaccc tgtctcccgc atcttcaaat atccggtccc gcattatcag cttgagggca
    22621 ttgatcgctt catccatgtc cgtggcaata aagacgccct tccctgccgc aagaccctca
    22681 gccttaatca cgatgggggt ccccttcatt ttcacatgat tttctgcatg gacatacgac
    22741 gaaaacacgc tgtactctcc tgttgggata ccgtaccgtt tcatgaaatc cttggcaaag
    22801 accttgcttg attcaagctg tgctgctttt tcactcggtc ccagtatcct catcccctgt
    22861 ttttcaaagg catccacaat gccccgggag aggggccctt caggccctac gatggtgaga
    22921 tctatccact catactttac aaaatccacc agggcgtcga aattgtttgt tcctatgtct
    22981 atgcattcgg ccagatctgc gattccggca ttcccaggtg agcagtagat tttgtccaca
    23041 tgccttgact gagccaactt ccatacaatc gcatgttccc ttcccccgcc accgatcacg
    23101 agaactttca tattacccgt tcctttctct cgcattgctc accagccgac acaccgtatt
    23161 acctgccgta tgagtgctcc ggagatgtgc tcccctcatt accagatgtc ttgagaatat
    23221 gatgtgcagt aattatacta tttcggattc ccctgtatct gttccccggt cttctcgcca
    23281 ttccggttcc ggactctttc cggcttcgtc cccagaaatt atgatacctt ttgctttctg
    23341 tcgagaatcc catcagtgtc tgaaatgtct taccccggta attatcatag ccatggaata
    23401 ctcgtccgcc gcctttatca cctcttcatc ctttaccgat ccaccgggct gcaccaccgc
    23461 agtcaccccg gcttcataaa gcacatcgat tccgtcccta aaggggaaga agccgtcaga
    23521 agctgccaca gaacctttaa caggctctcc tgcgttcaag aggccggtct gtgcagaaaa
    23581 aacccttttt gtctgaccga tgccgattcc tgcagtcctg tctttgaatg catacacaat
    23641 ggcattggat ttcacatgtt tacagacctt ccacgcaaag gacagtgcct cacgctcatc
    23701 ctttgtgggc tgacgtttag tgattgcctt aagcgccatg atgtcctctg tggaatagtc
    23761 ccatccctgg agcagtaagc cgccggcaat tctctttatg tcccatgagc cgggccgtct
    23821 tttatcaggc tggagcatat cgggcaaagc aaggagtctc acgttcggtt tgtgggagaa
    23881 gacctcaagg gcacctttgg taaaagaagg ggcgatgacc acctcaagaa agaggtccgc
    23941 catctctttt gcggccgctt catccacttc ggtattgaat gccacaaccc cgccaaaggc
    24001 cgaaacaggg tcggtttttg ccgcctttgt atatgcttca gcagcagttg cacccacagc
    24061 aaccccgcag gggttgttat gttttattat tgcgcaagcc ttctgattaa attccagcgc
    24121 aaggagcagc gcagagtgag tatccaaata gttattaaaa gacatctcct tgccctggag
    24181 gacttttgca tccggaaggg aaagaccctt agtcctctct ctgtagatgg cagccttctg
    24241 ggcagggttc tctccatatc tcaaggtagc gacccttcta agagtggtgg tgaaaaattc
    24301 agggaaagag ggttcctttt cggtgatgcc ggtgagataa tccgcaataa gtgtgtcata
    24361 gcgtgcagta tgggcaaaaa cttttttcgc aagattgagt tttgtctctt tgctcaaagc
    24421 ccctttggat gattttattt cacgaagtat ggcttcatag tcatcgggat ccactactac
    24481 ggtgacgtcc tgaaaattct tcgacgcagc cctgagcatg gtgggaccgc cgatgtcgat
    24541 attttcaatg gcctcttcaa agctcacacc gggcttcgag atggtctcct caaagggata
    24601 gagatttacc acaaccatat ctattggttt gatatcatgc tcttcaatat ccttcatgtc
    24661 tttaggattg cttcttctgg aaaggagccc tccgtgtatt tttgggtgaa gtgttttcaa
    24721 tctgccatcc agcatctccg ggaaccctgt atactcagac acctccacca ctgagacacc
    24781 tgcctcccgg agggattttg ccgtcccacc cgtggagagg atctccactc ccatgcccga
    24841 aagctccttc gcaaaatcaa cgataccttt tttgtttgag acgctcataa gtgctctttt
    24901 gatcatatgt tcctcccctc aggttttata attacacgat gatacagact tgaatgattc
    24961 cgcaaacgat tatatcacat tatccctttt tctttaaggc tcacgtatgt cccatcgccg
    25021 ataatcacat gatcaagaac cgtgatgccc acaatatccc ctgttgcctt cagtctttcg
    25081 gtaaccgcga tgtcatccct gctcggccct gggtctccgc tgggatggtt atggacaaag
    25141 ataactgaag ccgctgactc cctgatagcc tccttaaagg cctcacgggg gtggataagg
    25201 gaattggtga gtgttccctc cgaaatcttc acctcccgca ccaatctgtt ctttgtatca
    25261 aggagtgcgc agagaaaggt ctcctttttt gcatttttga accgggacgc aaaataggaa
    25321 tagacgagat tacttgagga aaatacaggg gtattcccta tgggctcgct catcagacgc
    25381 ttccccagct caaacgctgc cttcaactga gcaatttttg cctttcccag acccttcgtt
    25441 aaggagagat caacgataga ggcactgtca atatttttca atcccccaaa tttatcaagc
    25501 agttccattg caagggcaag aacgcttttc ccctctccac ctgtcctgat gattatcgca
    25561 aggagttggg catctgaaag gctttctgtg ccatacttaa tgagtctttc cctcggcctc
    25621 tcattttctg gccagtcttt aatcttcggg tttgccatct cactcccttc tagtggaagc
    25681 ctattttatc aattttgctt cattttttgt gcaaaacttt ctttgtcgtt ctcctacaaa
    25741 gattatctaa ggggtcggaa tataattccc atgctgttga ttaaaaaaac tattttctta
    25801 tttttcatta tctatcccgc aacctatttt tttgcctgca aaaattgcat aaaaaaaagc
    25861 aaaaaatgta tagaaattcc tttctgattt tgaaatactt acttcattag gatagaatcc
    25921 gaggacatga tcaggataat ttcaccatgg aaaagccatt tgtaggaaag ttgcagaatg
    25981 aagtgcatta ccaatgcgac acctgctatg ccattaaagt cctcaaatat gtggtaatgg
    26041 accatgcagg caacatcttg agtatttgcg aagaatgcag ggacaggatt ttggaagaga
    26101 gtaactgatt cttaaggatt cgagagttga atcagttgaa aactcaagca cttacaacta
    26161 ttttcgccaa tatcctgagc gcacacacat aggcaaagat tgtttgagaa agctaaatcc
    26221 ttgaattctc cccaccagtg aacactccta agacatacca ttaaatctcc tcacaccgca
    26281 ctctgttgtc aacaagtaga attgtccggt tttagagcaa atagattgtc cggtttctgt
    26341 agaggagggc acacgtggat cacggtagcc ctcctgaaag cgatttcacc gcgctcatgc
    26401 cgccttgtgc acatgctctg ggagttcctt tcctcgccta tcatatgagg cgagttttct
    26461 gggtccatga aagagagcaa gggagccatc gggatatcga tgcactctga ccctgacctt
    26521 aacgtaatgg cagcggtagc ggtcctgagg gatctgcagg atgaggccat tgaaccggac
    26581 acagttatca ttgcccacga cacgctcata ctgttcgcag aggatatcct tcaagggagc
    26641 tcctgcccaa gggacgaaca tactggcggg ctcctgaggg cagtggcaaa ctcggcattg
    26701 taggcaggca gatagatacg ggc
//
LOCUS       DATKOU010000201         7070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1062688, whole genome shotgun sequence.
ACCESSION   DATKOU010000201 DATKOU010000000
VERSION     DATKOU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062688"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VMU21_09390"
     CDS             <1..318
                     /locus_tag="VMU21_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUO77775.1"
                     /translation="KADKDKLMQVFVNLLENAVGAGSKRIEITSEKKDNGLGIAVKDD
                     GTGIKPDALERIFEPFFTTKKEGTGLGLPICRKIMEDHGGTMEVLSTAGAGTTFFLTF
                     RGE"
     gene            complement(326..1348)
                     /locus_tag="VMU21_09395"
     CDS             complement(326..1348)
                     /locus_tag="VMU21_09395"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HUO77776.1"
                     /translation="MSRDREIKVLIVDDSPFVRKALLRIFDSEPTISVVGVARNGREA
                     IEKTLSLKPDVLTLDIMMPVVDGIETLKIIMEQSPLPVLVLSQFTHEGAALTLKALEL
                     GAMDFVDKSSKGLMDFLALAAEIVSKVKAIAHNEPKKLVYEPRVLSGYRSQGIVDVVA
                     IGASTGGPPALEMILQKFPGDISFGVLIVQHMPKGFTATLAERLNSICAIRVKEAEDR
                     DKIEPGSALIAPSGLHMKAWKGGCVIRLEGEPLNLIHRPSVDVLFQSVGEVYRERSMG
                     VILTGMGSDGARGMGIIKDKGGATLAQDEETSVIFGMPKVAIKSGVVDKVVSLTEMAE
                     EIMKNA"
     gene            complement(1352..2236)
                     /locus_tag="VMU21_09400"
     CDS             complement(1352..2236)
                     /locus_tag="VMU21_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HUO77777.1"
                     /translation="MTSLSTHEQKTVGMTEDEYALFRDLIEKEFGIVMKGDKRLTLHT
                     KLSHRLSILDIRSYKDYYDFIISEPSKEEFYSFMAHITNNETYFQRELGRITLVAGLL
                     DDIKRQRQKKDQKRITFLSAGCSTGEEAYTLKIMLMETGLFAWGWEVKVIGIDVSRSA
                     VKKARNAVYTKNSFRRLDGTDEFSQKYFDRRGDLYTLKSAYRSQVEFRHGNILDPASW
                     GGIQDIDVIFCRNVLIYMSDNAIGRITENFYRHLADEGYLFIGSSESLLNKTDLLVPE
                     CRESLIIYRKNLKCSPKR"
     gene            complement(2238..3362)
                     /locus_tag="VMU21_09405"
     CDS             complement(2238..3362)
                     /locus_tag="VMU21_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO77778.1"
                     /translation="MEKIQTLLKELNSADWWARNAAIKGLFLHNEKSYLPALEKALKD
                     HENALLRNASMELYVALGARALPSLVALLKGDDPEVRIFAANLLGDIKDRRAVQDLMR
                     AMKDTDSNVRVASTEALGKLGAPEAVAALSEALNDEPWVVMSAIKSLGDIGGEEALQV
                     LYGCLERDEYRGITFDAIEMAGDEEAIENITPFVERDDTREWALKAVVNIAVRAGIRI
                     VPEYFVNLVPHLLALQHSPHPEIRKAAFIALAWSEDLRGIPSFINSLNDDELQEYAIS
                     AILAIREGAVPEIIDALRDPERPQRCMLADLLSMMGEHRALMQFSEDHDPEVRVAVAL
                     AAGTIHSSRTDEILTKMLDDPEDEVRLAAQKASRDLKRVR"
     gene            complement(3429..5795)
                     /locus_tag="VMU21_09410"
     CDS             complement(3429..5795)
                     /locus_tag="VMU21_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUO77779.1"
                     /translation="MKHYFMNMSIAKKFLVAPIVIMVFMILLGGITYRVFYTQQGALK
                     DIYKNRFGTYQVAAQISKDLANVHANIYKVIGWMDARYATEKVEALAKEQVAAMNQTT
                     KTIQEKIASPSTLPEEKKIFQGLLASLGDYKESAQATIDLGSSVTEFATMYMNVADEK
                     FIALNKELDALVAFEKKLSEESYQRSSNNFTTAMVAFLAGFILAILFSAGMMLFMKGL
                     VVSPISKLTEKLKEIAAGEGDLTKRVDISSDDEIGEMGKSLNLFLDHIHGLVSTIRGT
                     TLKTASVTNQIAVSSNQVMEGASTTARSAEQTLTSMEQMAASIAQVAKNTEFLATNVD
                     ETSATIGEMAASIEQVGRNADVMAASVGETSATIEQMTMSMEQSSKSTSSMTESVSDT
                     SMTVENLLSSVEQVSKGTESLKHMVTESSGTIEEMMRTVQEVATKIGDANKLSQNAYK
                     DAEQGGKGIYQSIESLQNIGKTTEKTMGIIENLGKRSEEIGSIVEVINEIADQTNLLA
                     LNAAIEAARAGDAGRGFAVVADEIRKLAERSMEATKEIGSVIKQVQGETAMAVKATEE
                     TYREGKGGMALASSSRDAFNNIIEAVKDTSQIMEGIARSAGELSKATGQVMNYIVEMN
                     SASGEVANAVKAQADGTGSIRNIIQKMNGQVQEVNIATKEQAIGGKQIREALERMKTV
                     VHEVNIAVKEQVKGARQIVESIEMMRSMTQDVANAAAEQKTGGDTVVKAMEGMSHIAS
                     ENLKLSADMKGASEDTLSQVESLQQSVSRFKIETNGNNPSRDQVLANV"
     gene            complement(5851..6324)
                     /locus_tag="VMU21_09415"
     CDS             complement(5851..6324)
                     /locus_tag="VMU21_09415"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HUO77780.1"
                     /translation="MKLKKTVVCIMVTMFLASLAFAAEKGPEKRGTKAEAEAMVKKAI
                     EYIKANGRDKAFAEFSNPSGKFVDKDLYVFVYDLNGKCLAHGANAKMVGKDLINMKDP
                     EGKEYVKERISIAKNQGKGWQDYKFPNPVTKQIEHKTAYIEKFEDIIVGCGVYKS"
     gene            complement(6502..>7070)
                     /locus_tag="VMU21_09420"
     CDS             complement(6502..>7070)
                     /locus_tag="VMU21_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77781.1"
                     /translation="EDAMYIRTKTSLIQTLVMAAPLFIILLIIYSSASGLVYEKGDAI
                     FNEKLHRVMSVIESKYDSLVKAGTENVEGYVKEAQKTLIGDLRANYYKDKSKDVNMFI
                     MDSDGGIVLHPTLQAGAHDMDSIAKTMLSGQARGSLTFQMNGEQRRMQYEYFKPWKWY
                     IGYVVEDSPKYAVIMSFLKVLVDGALVE"
BASE COUNT         1488 a         2056 c         1559 g         1967 t
ORIGIN      
        1 aaggccgata aggataaact gatgcaggtg tttgtgaatc tcctggaaaa cgcagtggga
       61 gcaggcagta aacgtataga gatcacctca gagaaaaagg acaatgggtt agggatagcc
      121 gtaaaggatg acggaacagg cattaagccg gacgctctgg agaggatatt tgagccgttc
      181 ttcaccacaa aaaaggaagg gacaggtctc gggcttccca tatgcaggaa aattatggaa
      241 gaccatgggg ggaccatgga ggttctgagc accgcgggcg ctggcacaac ctttttcctt
      301 actttcaggg gcgagtaatc aggagttacg catttttcat tatctcttcg gccatctcgg
      361 tgagagacac cactttatcc accaccccgc tttttatggc aaccttcggc attccgaata
      421 tcaccgatgt ctcttcatcc tgcgcaaggg tggcccctcc cttgtccttg ataattccca
      481 ttcccctggc accgtcagat cccatgccgg tgagaatgac ccccatggaa cgctccctgt
      541 acacctcacc cacggattga aaaaggacat ccaccgaggg cctgtggatg agattcaggg
      601 gttctccctc cagtcttatg acgcaacccc ctttccatgc cttcatatga aggcccgagg
      661 gcgcaatgag tgcacttccg ggctcaattt tgtccctgtc ttcagcctct ttcacccgaa
      721 tggcacatat gctgttgagc ctctccgcaa gggttgcggt aaaccctttg ggcatatgct
      781 ggacaatgag taccccgaag cttatgtccc cgggaaattt ctgaaggatc atctccagcg
      841 cgggaggacc ccctgttgac gcaccaatgg ccaccacatc cacaatgcct tgcgaacgat
      901 agccggaaag gacacggggc tcgtagacaa gtttcttcgg ttcattgtgg gctatggcct
      961 ttaccttcga gacgatctcc gcagccagtg caaggaagtc catgagcccc ttggacgatt
     1021 tgtccacaaa atccatggca cccagttcca gtgccttgag ggtgagagca gcgccttcat
     1081 gggtgaattg ggaaagcaca agcacaggga gcggactttg ttccataatg atcttcaatg
     1141 tctcgatccc gtcgaccacc ggcatcatga tgtcgagggt aaggacatca ggcttcaggc
     1201 tgagggtctt ttcaatagcc tccctgccat tccgtgcgac ccctactacc gaaatggtgg
     1261 gttctgaatc aaagatcctc aaaagcgctt tccggacaaa tggtgagtca tccacgatca
     1321 ggacttttat ctctcggtca cgggacatgg gctacctctt gggagaacat ttcaggtttt
     1381 ttctgtagat aataaggctc tccctgcatt ccgggacaag gaggtccgtt ttattcagca
     1441 ggctttctga tgaaccaata aagaggtacc cttcatcagc aaggtggcgg tagaaattct
     1501 ccgtaattcg tcctatggcg ttatcactca tatagatgag gacattccgg caaaagatca
     1561 cgtcaatatc ctgtatcccc ccccatgatg cggggtccag aatgttccca tgcctgaact
     1621 ccacctggga ccggtaagcg gatttaaggg tgtataggtc ccctctccgg tcaaaatact
     1681 tttgggaaaa ctcatcggtg ccgtccagtc tcctgaaaga gttttttgta tataccgcat
     1741 ttcttgcctt tttgactgcg ctcctgctca cgtcaatccc tatcaccttc acctcccacc
     1801 cccacgcaaa aagaccggtc tccatgagca taatcttcag ggtatatgcc tcttcaccgg
     1861 ttgagcagcc tgcagacagg aacgtgatcc tcttttggtc tttcttctgc cgttgtcgct
     1921 taatatcatc caggagccct gcaaccagag tgatcctccc cagctccctc tgaaagtacg
     1981 tctcgttgtt cgtgatgtgg gccatgaagc tgtagaactc ctccttcgat ggctctgaga
     2041 taatgaaatc ataatagtcc ttataagacc gtatatccag tatggagagc cggtgcgaaa
     2101 gcttcgtgtg aagggtaagc cttttgtcgc ctttcatgac aataccgaat tccttttcta
     2161 tgaggtccct aaagagggca tactcatctt ctgtcatacc cacggttttt tgttcatgtg
     2221 tgctcagact ggtcacgcta tcgcaccctt ttgaggtcgc ggctcgcctt ttgggcagcc
     2281 agccgtacct cgtcctctgg atcatcgagc attttcgtga gtatctcatc ggttcttgag
     2341 gagtgtatcg taccggcggc aagggccacc gcgaccctga cctcgggatc atggtcttcg
     2401 gaaaactgca tcagcgccct atgctcaccc atcatcgaga gcagatcagc aagcatgcat
     2461 ctctgtggtc gctcgggatc tctcagtgca tcaataatct ccggtaccgc cccttcccta
     2521 atagccagga tggcgctgat ggcatactcc tgaagctcat catcattcag tgagttgatg
     2581 aaggagggaa ttcccctcaa atcctcggac caggccaacg cgataaatgc tgcctttctt
     2641 atttccggat ggggcgaatg ctggagagca agcaggtggg gcaccagatt gacaaagtat
     2701 tcaggtacta tccttatccc tgctcttacg gcaatgttta ccacagcctt cagcgcccac
     2761 tctcttgtat cgtcccgttc cacaaatggg gtaatattct ctattgcctc ttcatcacct
     2821 gccatctcta ttgcatcaaa ggttatgccc ctgtattcat ccctttcaag acatccgtag
     2881 agcacttgga gagcctcctc tcctcctatg tcaccaaggg acttgatggc tgacatgacc
     2941 acccagggtt catcgttcag ggcctcgctc aaagcagcca cggcttcagg tgctccgagc
     3001 tttccaaggg cctcagtgct ggccactctg acattactgt cggtgtcctt cattgccctc
     3061 attaagtctt gaacagctct cctgtcctta atgtccccca gaaggttagc ggcaaagatt
     3121 ctcacctcag gatcatcgcc cttcaaaagg gccacaaggg atggtaatgc acgcgcaccc
     3181 agggcaacgt agagctccat agaagcattt ctcaagagtg cgttctcgtg gtcctttagt
     3241 gccttctcga gagccgggag atagctcttc tcgttgtgga ggaacaggcc ctttattgcc
     3301 gcatttcttg cccaccagtc agcgctgtta agttccttaa gtaatgtctg gatcttttcc
     3361 atattcagat tacaaggcca gcccttgaaa cgggtagcga gggcatcctt gggaatatct
     3421 tcctgcgttc acacatttgc caacacctga tctcttgaag ggttatttcc gttggtctct
     3481 atcttgaacc tgctcaccga ttgctggagg ctctccacct gtgagagtgt atcctctgag
     3541 gcacccttca tgtccgcgga gagtttcagg ttctccgagg cgatgtggct catgccctcc
     3601 atggccttca ccacagtgtc tcctcccgtc ttctgttcag ccgctgcatt ggccacgtcc
     3661 tgggtcatgc tcctcatcat ctcgatggac tctacgatct gacgcgctcc cttcacctgc
     3721 tccttcaccg ctatgttcac ctcatgcacc acagtcttca tccgctcaag ggcctcacgt
     3781 atctgcttgc cccctattgc ctgctccttg gtggcgatgt tcacctcctg tacctgcccg
     3841 ttcatcttct gaatgatgtt ccttattgag ccggtgccat ctgcctgtgc ctttaccgca
     3901 ttggccactt cacccgaggc actgttcatc tccactatat agttcataac ctgccctgtt
     3961 gccttgctca gttctcctgc tgatctcgct atcccctcca tgatctggga agtatccttc
     4021 accgcctcga taatattgtt gaaggcatca cggctgctgg aggcaagtgc catgcccccc
     4081 ttaccctctc ggtacgtctc ctctgtggcc ttcaccgcca tcgctgtctc tccctgtacc
     4141 tgtttgatca ccgaaccaat ctcctttgta gcctccatgg accgctccgc cagcttccga
     4201 atctcatccg ccaccaccgc aaaccctctt cccgcatccc ctgcccttgc cgcctctatg
     4261 gcagcgttca gcgccagcag gttcgtctgg tctgcgattt cattgattac ctcaacaatg
     4321 ctcccgatct cctcggacct cttccctaaa ttctctatga tccccatggt cttctctgtg
     4381 gtcttcccta tgttctgtaa actctcgatg ctctggtaga tccccttgcc cccctgctcc
     4441 gcgtctttgt atgcgttctg gctcaacttg ttcgcgtccc ctatcttggt ggccacctcc
     4501 tgcacggtcc gcatcatctc ctcgatggtc cccgagctct ctgtcaccat gtgcttcagt
     4561 gactctgtgc ccttggatac ctgttccacc gacgacagga ggttctccac ggtcatcgag
     4621 gtgtcactca ctgactcggt catggatgag gtgctcttcg atgactgctc catagacatt
     4681 gtcatttgct cgatggtggc tgaggtctcc ccaaccgagg ctgccatcac gtccgcgttc
     4741 ctccccactt gctcgatgga tgccgccatc tcgccgatgg tggctgaggt ctcatccaca
     4801 ttcgttgcca ggaactcggt gttcttcgcc acctgcgcta tggatgccgc catctgctcc
     4861 atgcttgtga gggtttgctc cgctgacctt gctgtggtgg acgccccttc cattacctga
     4921 ttggagctta ctgcgatctg gttcgtcact gaggcggtct tcaacgttgt cccccggata
     4981 gttgagacaa gtccatgaat atgatcgagg aagagattca ggctctttcc catttctccg
     5041 atttcgtcat cagaagagat atccactctc tttgtgagat caccctctcc tgcggcaatc
     5101 tccttaagct tctctgtgag cttgctgatg ggagatacaa caaggccttt catgaataac
     5161 atcatccctg ccgagaaaag tattgccaga atgaatcctg ccagaaacgc caccattgcc
     5221 gtggtaaaat tgttcgatga acgctggtag ctctcttcac tcagtttttt ctcaaatgcc
     5281 acgagtgcgt caagctcctt attgagtgca atgaacttct cgtctgcgac gttcatgtac
     5341 atcgttgcaa actctgtcac cgaactgcca aggtcaatgg tcgcctgtgc tgattctttg
     5401 tagtccccca gagaagcgag gaggccttga aagatctttt tctcttccgg gagcgtcgaa
     5461 ggagaggcta ttttttcctg gatagttttg gttgtctggt tcattgccgc aacctgttcc
     5521 ttggcaaggg cctccacttt ctcggttgca taccgggcat ccatccaccc gatcacttta
     5581 tagatgtttg cgtggacatt ggcaaggtcc tttgagatct gcgcggcaac ctgataggta
     5641 ccgaaccggt tcttatagat atccttcagt gccccctgtt gggtgtagaa cacacggtat
     5701 gtaatacctc cgagcaggat cataaatacc ataatgacta tgggggccac aagaaatttt
     5761 tttgcaatgc tcatattcat aaaatagtgt ttcataacac ctcctctgat gaatttcatg
     5821 gccggggtga tcgccccggc catgaatctg ttacgacttg tatacgccgc agcccacaat
     5881 gatatcctcg aacttctcga tatacgcggt cttgtgctct atctgcttcg ttacaggatt
     5941 gggaaatttg taatcctgcc atccctttcc ctggttcttg gcaatgctaa tcctctcctt
     6001 cacatactct ttgccctccg ggtccttcat gttgatgagg tctttcccca ccatcttcgc
     6061 attcgcaccg tgggccagac attttccgtt caggtcatac acaaacacat agaggtcctt
     6121 gtccacaaac ttcccactcg gattgctgaa ttccgcaaag gccttatctc tcccattcgc
     6181 ctttatgtat tcgatggctt tcttcaccat cgcctctgct tcagccttcg ttcccctctt
     6241 ttcaggacct ttttccgctg caaatgcaag acttgccaaa aacatagtta ccataataca
     6301 caccactgtt ttcttgagtt tcatcttgtt gtctcctttt cctcattcat ggttcatact
     6361 cggcaaactt ccttgccttc ccataacgtc catatatttt ctgctattac atcacctcct
     6421 taaatcacat gcttgcatat ctttacccgc ctccctcatt tgcctcgata ctctgttctt
     6481 atatacatag catcctccgc ctcattccac taaggcacca tcaactaaga ccttcaggaa
     6541 gctcataatc accgcgtatt ttgggctgtc ttccaccaca taccctatat accacttcca
     6601 tggcttaaag tactcatatt gcatccgtct ctgttcgccg ttcatttgaa aggtaaggga
     6661 cccccgtgcc tgaccagaca gcatcgtctt ggcgatgctg tccatatcat gggcacctgc
     6721 ttgaagcgtt gggtgcagaa cgataccgcc atcgctatcc atgatgaaca tattcacatc
     6781 tttggacttg tccttgtaat agttcgccct caagtccccg atgagggttt tctgcgcttc
     6841 tttgacgtat ccctcaacat tttccgtgcc agcctttacg agggaatcat acttgctttc
     6901 tatgacggac atcaccctat ggagcttttc gttaaagatg gcatcgccct tttcatagac
     6961 caatccagag gcggaagagt agatgatcag caaaatgatg aacaggggag cagccattac
     7021 caatgtttga atcagacttg ttttggtcct tatatacata gcatcctcct
//
LOCUS       DATKOU010000202         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1824296, whole genome shotgun sequence.
ACCESSION   DATKOU010000202 DATKOU010000000
VERSION     DATKOU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824296"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="fabD"
                     /locus_tag="VMU21_09425"
     CDS             <1..878
                     /gene="fabD"
                     /locus_tag="VMU21_09425"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HUO77782.1"
                     /translation="ISENFPEAKEVFEEASSALGYDVAQMAFNGPTEELNKTFRTQPC
                     ILTVSTAIQKLLITKGVKPSVVAGHSLGEYSALVAAGVLPFQDAVKLTEKRGQFMQEA
                     VPEGKGLMAAILGLQRSKVDEICLSLQSGYASSANYNCPGQIVIAGEKEAVEEAIKLA
                     KEAGAKRAMPLAVSVPSHCTLMADASKRLGKLLEGIEFRNPVISIVNNADAMFLNNVE
                     SIKGSLVRQLSSPLLWEDSIRVIADSGVDTFVEVGPGKVLSGLIKRIEETAKTFHVED
                     IKSLEKTLAELTTKS"
     gene            complement(1181..1408)
                     /locus_tag="VMU21_09430"
     CDS             complement(1181..1408)
                     /locus_tag="VMU21_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77783.1"
                     /translation="MKRFSLFLVAVMVMLSLSLIGLPVVTEAAISDKKCVKGGGTVET
                     QDSKSICKGGKYDGKKVKVKKTKQTEQKPQQ"
     gene            2009..>2324
                     /locus_tag="VMU21_09435"
     CDS             2009..>2324
                     /locus_tag="VMU21_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HUO77784.1"
                     /translation="MKKLGFMVLVLLLVAGIAYAKDYEVKKKAGDFDVQVAIDKNPPV
                     VGDNNVKIEIKDSAGKYVSDAKVVVDYGMPAMPGMPAMNYKADAELKGNEYKAKMNLS
                     MSG"
BASE COUNT          605 a          495 c          632 g          592 t
ORIGIN      
        1 agatttccga aaactttccc gaggcaaaag aggtatttga ggaggcatcc agtgccctgg
       61 gctatgacgt cgcgcagatg gccttcaacg ggcctacgga ggagttgaac aagaccttca
      121 ggacccagcc ctgcatcctg accgtgagca ctgcgatcca aaagcttctg ataacgaagg
      181 gcgtcaaacc ctctgtagtg gccggacaca gcctgggaga gtattccgca cttgtggcag
      241 caggtgtctt gccttttcag gacgcggtga agcttacgga aaaaaggggg caattcatgc
      301 aggaggcggt gcctgagggt aaggggctga tggcagcaat cctgggactg cagaggagca
      361 aggtggatga gatatgcctt tcgctccagt cagggtacgc atcttccgct aattacaatt
      421 gtcctggtca gatcgtgatt gcaggagaga aagaggcagt ggaagaggcc ataaaactgg
      481 caaaagaggc aggggcaaaa agggcaatgc ccctggcggt gagtgttccc tcccactgca
      541 cgttgatggc agatgcgtcc aaaagattgg ggaaactact ggagggaatt gaatttagga
      601 atcccgtaat ttccattgtg aataacgcag acgcgatgtt ccttaataac gtagagagta
      661 taaagggttc ccttgtacgt cagctcagca gtcccctgct gtgggaggat tccatacggg
      721 ttattgcgga ttccggggtg gacacctttg tggaagtcgg gccggggaaa gtgctctctg
      781 ggttaatcaa gaggatcgaa gaaacggcaa agacatttca tgtcgaagac ataaagagcc
      841 ttgaaaagac attggcggaa ctaacaacca agagctaata acttttgtta ccagcttctc
      901 ccacccccac cgccgaagcc gccccctgaa gaaccaccac tgaatccgct ccaaccaagt
      961 caagagaaag tcatcatacc atgagagagt tgctgtcata cattgcatgt atcaaggggt
     1021 gagttacagg tggaaagaat ctggggaggc gccactgaat cggttctcgt gtgccgaatg
     1081 aaggaatgcc ccgtgcgtga gacggggcat tccttcatta cgttacgcac ctgagagaac
     1141 accagtgtgg ttctatgggt gtccatctct tttgcccttg ttattgctgc ggtttttgtt
     1201 cggtttgctt ggtcttttta accttcactt tcttgccatc atattttccg cctttgcaga
     1261 tggatttact gtcttgtgtt tcaaccgttc cgccaccctt gacgcacttt ttatcgctga
     1321 tggctgcttc agtgacaacg ggcaatccta tcaggcttag cgagagcatc accataactg
     1381 caacgagaaa taatgagaat cttttcattc ctttcacctc cttcttacgc tcatacgagc
     1441 ttctctcctt tgtttcctgt actcaggata acaacttggt tgtctattat actttgttcc
     1501 attatttttg caaacgcaaa tatccccggg agccctgaat caggtctctt tactgaaagc
     1561 tcacctttgc cggcttcctt tccacgtcag gcgcttccag ttcgaagaac tcctcttgaa
     1621 cgaagacgca atcacattgg aaggaaaaga ctggataagg gtgttgaggt cccggacgac
     1681 cggtacggga gatattagca gattttctgg aacagggtat aaggaatatt tttgtggcga
     1741 acgttttctt catatttgtt tcatcattga atagtagagt gatagctgag gattggaatg
     1801 atcatatgag agggaggtga taaaaaagat ttgagcaggc agtgcgaggg tctcctcagc
     1861 tgttacgttg acaaccatgt ggtgatatga catatttgtc ttggggagct ttgaggacct
     1921 tccggtttta ctagagtggt aataaggata tgaaaaactg ttgaacggga acataaacgt
     1981 tagatacaac ctataaggag gtttgacaat gaaaaaatta gggtttatgg tgttggtcct
     2041 gcttctggtg gcggggattg cgtatgcgaa ggattacgag gtgaagaaga aggcgggtga
     2101 ttttgatgta caggtggcca ttgacaagaa tccgcccgtg gtgggtgaca ataatgtaaa
     2161 gatagagatc aaggattccg caggaaagta cgtgagcgat gccaaggttg tagttgatta
     2221 tggcatgcct gccatgccgg gtatgccggc aatgaattac aaagcggatg cggagctcaa
     2281 gggaaatgag tataaggcca agatgaatct ctccatgtcc ggat
//
LOCUS       DATKOU010000203         3777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5292447, whole genome shotgun sequence.
ACCESSION   DATKOU010000203 DATKOU010000000
VERSION     DATKOU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5292447"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VMU21_09440"
     CDS             <1..513
                     /locus_tag="VMU21_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HUO77785.1"
                     /translation="YCLMPNHIHLLTRPFSEGSLQKMMQGITLCYTQHINRKYRRTGR
                     LWESRYHSCIVDQEAYLWAVIRYIEQNPVRADIVKNAQDYRYSSASAHLGLVYDPVLG
                     EDLFPEIQRDDYTEFMQTGIPAEQIKEIRQSARTGRPLGTKKFVTEMENILGKRLAAL
                     AHGRPRKKQP"
     gene            complement(652..1563)
                     /gene="cysK"
                     /locus_tag="VMU21_09445"
     CDS             complement(652..1563)
                     /gene="cysK"
                     /locus_tag="VMU21_09445"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HUO77786.1"
                     /translation="MRAKTILETIGDTDHVRINRLFSDHVEVWMKLERSNPGGSIKDR
                     IALSMIEDAESKGILNKESIIIEPTSGNTGIGIAMVAAVKGYRLILVMPESMSLERRK
                     LMSIYGAEFVLTPKEKGMKGAVDRATEMARETPNAWMPQQFENQSNTEAHKKFTAKEI
                     LRDFPEGIDYMITGVGTGGHITGCAQILKKEFPKLKVYAVEPALSPVLSGGNPGPHPI
                     QGIGAGFIPRILERKLLDGVIQVSKEEAFEYTRRCASEEGILVGISSGASLAAVAHKI
                     KEAHRGSRILTFCYDTGERYLSVEGLF"
     gene            complement(1790..2260)
                     /locus_tag="VMU21_09450"
     CDS             complement(1790..2260)
                     /locus_tag="VMU21_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HUO77787.1"
                     /translation="MGTQYRLMKSEPSVFSISDLKARPRSTDHWDGVRNYQARNYMRG
                     MKKSDLVLFYHSNCEMPGVVGLAEVVREAYPDHTAFDPLSPYYDAKSLQGNPRWFMVD
                     VQWKEDFRRTVPLGEMRNTPALKGMKVLEKGQRLSVMPVTRQEFEKVCALGRAP"
     gene            complement(2284..3192)
                     /locus_tag="VMU21_09455"
     CDS             complement(2284..3192)
                     /locus_tag="VMU21_09455"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUO77788.1"
                     /translation="MTRKIRKLWKSKPTVEGAGVHLKRAFGYYQVPQLDPFLLLDDFH
                     SDNPRHYLPGFPWHPHRGIETITYVLHGKVEHGDSMGNSGIISPGDVQWMTAGSGIIH
                     QEMPKEEKGKLLWGFQLWANLPSAHKMMDPRYRDVKSSQIPEVTLSSGVTVKIICGRI
                     NGNTGPVKDIVTDPEYIDVSVPAETTFTHRVPSGHTVFAYVVEGEGYFDKERNPFAHE
                     VIGDNYFDFKRDCLCRPETLVLYEQGGEEVVITTEHSQVRLLLVSGRPIGEPVAWYGP
                     IVMNTQEELKTAFEEFEKGTFIKYKT"
     gene            3396..>3777
                     /locus_tag="VMU21_09460"
     CDS             3396..>3777
                     /locus_tag="VMU21_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUO77789.1"
                     /translation="MKRQDLRNIAIIAHVDHGKTTLVDGMLRQSGIFRWNEKVQERVM
                     DNIDLERERGITIMAKNTAVEFMGTKINIVDTPGHADFGGEVERTLMMVDGVLLLVDA
                     SEGPLPQTRFVLKKALELNLPPILV"
BASE COUNT          888 a          970 c          978 g          941 t
ORIGIN      
        1 tactgcctta tgccaaatca tatacatctt cttacaagac ccttttcaga agggtctctt
       61 cagaaaatga tgcaggggat aaccctctgt tatacccagc acataaacag gaaatatcgt
      121 cgcacaggga ggctctggga aagcagatat cactcatgca tcgtagatca ggaagcgtat
      181 ctgtgggcgg ttatccgcta tatagaacag aatccggttc gcgcagacat agtaaagaat
      241 gctcaggatt atcggtattc cagcgcgtct gcccatttgg gccttgttta cgaccctgta
      301 ttgggagaag atctatttcc tgagattcaa agggacgatt acaccgagtt catgcaaaca
      361 gggattcctg cagaacagat taaggaaatc cgtcagagtg caaggacagg aagaccatta
      421 ggaaccaaga aatttgtcac agaaatggag aacatactcg ggaaaagact tgccgctcta
      481 gcccatggaa gaccgaggaa aaaacagcca taacatatca gaaaacggga tgtgtcccca
      541 tttttaaaaa gtgaggctga caagatctgc ccctgcatat agaacgcacg taacgtataa
      601 gatttcaaca cacgcaagaa agcaagttcc acagggcaaa cgtgcaggga ctcagaagag
      661 accctctact gaaagatacc gctccccggt gtcgtagcag aaggtgagga tacggcttcc
      721 tcgatgtgct tcttttattt tgtgagccac tgcggcaagg gaagcccctg aagatatgcc
      781 caccaggatt ccctcttcac tggcacagcg tcgtgtgtac tcgaatgcct cttctttgct
      841 cacctgtatc accccgtcca gaagtttcct ttcgagaata cgcggaatga atccggcgcc
      901 tatgccttgg attgggtggg gtcccggatt tccgccgctg agcacaggtg acagtgcagg
      961 ttccactgca tagactttga gcttgggaaa ctcttttttg aggatctgcg cgcatcctgt
     1021 gatgtgtcct cctgttccca cgccggtgat catataatca atgccttcgg gaaagtccct
     1081 gaggatttcc tttgcggtaa attttttgtg tgcctccgtg ttggactggt tttcgaattg
     1141 ctgcggcatc caggcattgg gcgtttccct tgccatttcg gtggcccggt ccaccgcccc
     1201 cttcatcccc ttctcttttg gggtgaggac gaactccgct ccgtagatgg acatgagttt
     1261 gcgccgctcc agggacatgg actcgggcat gaccaggatg agcctgtatc cctttaccgc
     1321 cgcaaccatg gcaatgccta ttcccgtatt gcctgaggtt ggttcaatga tgatactctc
     1381 tttgttgaga atacccttgc tctcagcatc ttcgatcatt gagagtgcga ttcggtcctt
     1441 gatgctcccg ccgggattgg aacgctccag tttcatccag acctccacat gatcactgaa
     1501 taacctgttg attcttacgt ggtcggtgtc gccaatggtt tcgagtatgg tcttcgccct
     1561 catttcaacc tcctcatggg tcactgcgta atgtttatcg tatttgcctt atgcgtagta
     1621 ttttactgca agctatcctc ttgacacaat ggagcaaccc tgaggcaaag taggctacca
     1681 ggaaagacgg tgaatgagaa tgagggagaa tggtcccaag gcacatagcg cacaagatag
     1741 ggagatggaa aagctttatc ctggaagcat gaaagtcgcc atgaaaattt cagggcgccc
     1801 tgcccaaggc acaaaccttt tcgaactcct gtcttgtgac cggcataacc gagaggcgct
     1861 gacccttttc caggactttc atccctttca gtgccggcgt attccgcatc tcaccaagag
     1921 ggactgtcct ccgaaagtcc tctttccatt gcacatctac catgaaccac cgagggtttc
     1981 cttgcaggga cttcgcgtca taataggggg acaagggatc aaaggccgtg tggtcagggt
     2041 aagcttcgcg cacgacttcg gcaagaccca ctacacccgg catctcgcag ttggaatgat
     2101 aaaagagcac caaatcactt tttttcatcc cgcgcatgta gttcctcgcc tggtagttcc
     2161 tcaccccgtc ccagtggtcg gtggagcggg gacgagcctt cagatcgcta atggagaaga
     2221 ctgagggctc tgatttcatc agccggtact gtgttcccac ctttgttgct tctcccccaa
     2281 cgcttaggtt ttgtatttga tgaaggtgcc tttttcgaac tcctcaaagg ctgtcttcag
     2341 ctcttcctgg gtgttcatga caatgggacc gtaccatgcc acgggttcac cgatgggtct
     2401 gcccgagacg aggaggaggc gcacctgaga gtgttctgtg gtgatgacga cctcttcacc
     2461 cccctgttca taaaggacga gagtttccgg ccggcagagg cagtctctct tgaagtcgaa
     2521 atagttgtca ccgatcactt catgggcaaa gggattccgc tctttatcaa aatagccctc
     2581 accctcgacc acatacgcaa aaaccgtatg gcctgagggt acccgatggg tgaaggtggt
     2641 ctctgcagga acggacacgt cgatatattc tggatcggtg acgatgtctt ttactggtcc
     2701 ggtgttaccg ttgattcttc cgcagatgat cttgactgtt acaccggagg acagagtgac
     2761 ctcagggatc tggctgctct tcacatcccg gtaacggggg tccatcatct tgtgcgctga
     2821 gggaagattg gcccagagct ggaatcccca taaaagtttt cccttctctt ccttaggcat
     2881 ttcctggtga atgatgccgc tccctgcggt catccactgc acatccccgg gagagatgat
     2941 gccgctgttc cccatgctgt cgccgtgctc caccttgcca tggagcacat aggtgatggt
     3001 ctcgatgccc cggtggggat gccagggaaa gcccgggagg taatgtcggg gattgtcgga
     3061 atgaaaatca tcaagcaaaa ggaagggatc cagctggggg acctgataat agccgaacgc
     3121 cctcttgaga tgcacgcccg ccccttccac agtgggcttg ctcttccata gtttcctaat
     3181 cttccttgtc atggccttcc tccaaggtga agatgttttt tttattttat cagcttttca
     3241 tgctgcgcgc atgttgcttt tttctctcct cagcgattaa actattaagg aattcattca
     3301 tcctcagtaa gaacccctga acgctgccct atgaggtatt cttgcctaag agcgtgccgg
     3361 aagttttaat ctaagaggaa acaggagagg tgcaggtgaa acgtcaggat ttacggaaca
     3421 ttgcgattat cgcccatgtg gaccacggca agaccaccct tgtggacgga atgctcaggc
     3481 agtccggtat tttcaggtgg aatgaaaagg tgcaggaaag ggtaatggac aatatcgacc
     3541 tggaacggga gcgcggaatc accattatgg cgaagaatac ggcggtggag ttcatgggaa
     3601 caaagatcaa catcgtggat acgccggggc acgcggactt cggaggcgag gtagagagga
     3661 cactgatgat ggtggacggc gtgctccttc tcgtggatgc atcagaaggc ccgttgcccc
     3721 agacacggtt tgtcctcaag aaggccctgg agctgaacct gccgcccatc ctggtga
//
LOCUS       DATKOU010000204        18526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5293275, whole genome shotgun sequence.
ACCESSION   DATKOU010000204 DATKOU010000000
VERSION     DATKOU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5293275"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(539..955)
                     /locus_tag="VMU21_09465"
     CDS             complement(539..955)
                     /locus_tag="VMU21_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUO77790.1"
                     /translation="MEVKVKYIDGLQFVGEAESGHAVVMDGEQEYGGKNTAVRPTELL
                     LVGLGGCSGMDVVSVLKKKKQDIRGLEIHIKGKRAEEHPKKFTAIDIEFVVTGHSISE
                     EAVKRAVNLSMEKYCSVKATIEGCATITFSYRIVEG"
     gene            complement(1034..1816)
                     /locus_tag="VMU21_09470"
     CDS             complement(1034..1816)
                     /locus_tag="VMU21_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HUO77791.1"
                     /translation="MRVLFIGDIVGTVGRMVVKALVPTLADRYKVDCVIANGENAAGG
                     FGITEKVAEELFSCGIHVITSGNHIWDKKETVSYIGKENRLIRPLNYPPGVPGYGSIL
                     YPLPHGTKIAVLNLSGRVFMPSIDCPFRVGLDEIKRMQNETRFILVDFHAEATSEKIA
                     FGYFVDGKVSAVIGTHTHVQTADEKILPGGTAYITDVGMTGPEVSVIGIEKEQIIERF
                     LYQMPKKFETAKGNGMLSAVVIEMDDKTGKSTVIQRLQLTYP"
     gene            complement(1895..3460)
                     /gene="rny"
                     /locus_tag="VMU21_09475"
     CDS             complement(1895..3460)
                     /gene="rny"
                     /locus_tag="VMU21_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HUO77792.1"
                     /translation="MMFDALYLLITVLIGISLGLLLNLIYKRSLRSQQSALKKEREDI
                     IAEAKKAADVVKKEAAVEAKDVVYQAKSDAEKELRERRHELNQLDRRLRQKEETLERR
                     VSQAEKREHELNKREKEYNSKERGLQDKENHYHQIIKEQMVVLERVSGLSTEDARQEF
                     LRRVEEETRFEASKLAKRLEDEAREQAEKKAKEIVSLTIQRYASDYVADGTVTAVSLP
                     NDEMKGRIIGREGRNIRALEAATGVDLIVDDTPELVTLSAFDPVRREIARLSLERLIA
                     DGRIHPTRIEEIVEKVKKEVDATIKEEGDKAVFDLGLSGIHPEIIKLVGRLKYRTSYG
                     QNMLQHSREVAYLAGTMAAELKVDIKLAKRGGLLHDIGKAVDHEMEGSHQEIGANIAK
                     KYGENQKVINAIFAHHGDEEPTTVESALVAAGDALSAARPGVRRESIENYLKRLEKLE
                     QMAMSYNGVEKCYAIQAGREVRIIVKPEDVTDEMASMLSRDLSKKIESEMTYPGQIKV
                     TVIRESRYVEYAK"
     gene            complement(3705..3894)
                     /gene="ssrS"
                     /locus_tag="VMU21_09480"
     ncRNA           complement(3705..3894)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VMU21_09480"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4053..4856)
                     /gene="amrB"
                     /locus_tag="VMU21_09485"
     CDS             complement(4053..4856)
                     /gene="amrB"
                     /locus_tag="VMU21_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HUO77793.1"
                     /translation="MRRKPAVAGQFYHESSTRLSHQVRQYVADDLPKEHALAILSPHA
                     GLVYSGAVAGEVYSRIIIPETFLLLGPNHTGLGARMSLMPQGEWEIPMGIFQIDEQLG
                     RKILSNSPFVSGDSQAHMFEHSLEVQLPFIAHFSKTLKIVPLTIMSATIDECKNLGEG
                     LAKSIKETDYPVVIVASSDMSHYVSDGVARKKDKMAIDKILSLDPEGLFAVVRKEQIS
                     MCGYLPATIMLFAVKSLGAREARLVRYATSGDVSGDYDHVVGYAGILIV"
     gene            5203..5394
                     /locus_tag="VMU21_09490"
     CDS             5203..5394
                     /locus_tag="VMU21_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77794.1"
                     /translation="MAMYRTKKLVCVLLESLLYKKLSLKEKESLLMKVAENYPFPAEA
                     EEERAIGYESSWKGVIHLH"
     gene            5699..9133
                     /locus_tag="VMU21_09495"
     CDS             5699..9133
                     /locus_tag="VMU21_09495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HUO77795.1"
                     /translation="MHQHSDYVPLHLHTEYSLLDGSIKIDELAELASRYKMPAVAMTD
                     HGNLFGAIEFYKKISKAGIKPIIGCEVYVAPSGHLEKKGVTGGETPFHLILLARDNAG
                     YRNLVTLVSKAYTEGFYYKPRIDKDLLAQYSGGLIALSACLKGEVPSYVNKDMVDKAR
                     QCALEYKHILGSENFYIEIQDNGLKVQEKANKKLIELARELHVGIVATNDCHYLRKED
                     ARAHDILLCIQTGKTESDSNRMRFESNEFYFKSPEEMKRAFSELPDALLSTREIAERC
                     NVEFTLGKSLLPMYTREDGVSPEVFLKQLAAEGLRKKFGNNPPDSYRTRFRTELDIIN
                     RMNYASYFLIVWDFISYARENRIPVGPGRGSVAGSLVAYCLGITEIDPLRYNLLFERF
                     LNPERVSMPDIDVDFCKDRRGEVISYVSGKYGNDHVAQIITFGTMAAKAAIRDVGRSL
                     DVPYAEVDRIAKLIPNTLNISIDEALRGEPQLKALYESNAKVKEILDIAKRLEGLCRH
                     ASTHAAGVVVSPEPLTEYAPLYKNPTDGTITTQFDMGSVEAIGLLKFDFLGLKTLTVI
                     EKTLGYLKAGGREISLHEITLEDPETYRLLSSGQTTGVFQLESPGMRDILIRMQPNRF
                     EDLIALVALYRPGPMAWIDDFIKRKKGEAAISYKMPQLKEFLDETYGIILYQEQVMMI
                     ANKIAKFSMGQADILRRAMGKKKPEEMEKQKESFIKGAVGNGITEKKAAQLFETMAPF
                     AMYGFNKSHSAAYAYIAYQTAYLKAHYPVEFMAATLSTDTDNTDKIVKSIAECRKMSI
                     EILPPDINESNREFKVIGKSIRFGLEAVKGVGSSAIETILAARMEDGPFKTMEDFLRR
                     VDGKKVNKKVIESLIKAGAFDSFGMQRAKALEILSTVLNSDTSVSSLFAQQDIFDEGS
                     ERQGKEWDEMELLRYEKEALGFYITGHPLSRYRESLSAMKVREISHLEDVADKEEVQI
                     AGVISSVKKIKTKGKAEIMAYLTIEDEEGSMEAIVFPDLYRTMLDMFSKESVIVIKGT
                     IDKTEKGSKLITREAYRLDDFLNAVPLRLSKHSPTAQNGMQRMEITLAAHPAAREGLR
                     TLKELISDYPGRCPLSLRIELNGSYALMDTALHVEPSSVLVERIEGLMGKGAVRIR"
     gene            9203..10201
                     /locus_tag="VMU21_09500"
     CDS             9203..10201
                     /locus_tag="VMU21_09500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HUO77796.1"
                     /translation="MKYYLDFEKPIEELETKIEELKRLSDGKDIDITSEVRKLERKAK
                     DLRSEIFSHLTPWQKTLVARHPDRPYTLDYIDLLSEDFIELHGDRRFADDPSVVGGFG
                     RIRNTTFMIVGHQKGRGTKERILRNFGQPHPEGYRKALRLMKLAERFKRPVLTFIDTP
                     GAYPGIGAEERGQAEAISTNLMEMSRLKTPIISVLIGEGGSGGGIALGVADRIYMLEY
                     SVYSVISPEGCAAILWRKSGDLGTAEFTKAADALKLTAQDLFRFKVIDEVIPESSGGA
                     HRDPSGTAEKVADMVMKALEELKTKTPARIVEERYRRLRKIGVFEETAAASSNKGA"
     gene            10541..10951
                     /locus_tag="VMU21_09505"
     CDS             10541..10951
                     /locus_tag="VMU21_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77797.1"
                     /translation="MESRKTFVCKRCRKSPLCFEHMDREYKLCSACAAEERLTLYQNL
                     ATQERNVKSFLRLMQFILTLVVIFYGVKRFFYELVPDPLKVNVFFDYLFLWGAISLAG
                     MALSYVLLISQGQKIREIEDKIRDHKAYTKYLRR"
     gene            complement(11170..11607)
                     /gene="mscL"
                     /locus_tag="VMU21_09510"
     CDS             complement(11170..11607)
                     /gene="mscL"
                     /locus_tag="VMU21_09510"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HUO77798.1"
                     /translation="MLKEFKEFIMRGNVVDMAVGIIIGAAFGKIVTSFVNDILMPTIG
                     LMLGKVDFSNLFINLSGQHYPTVAAAKAAGAATLNYGIFFNTTLDFIIIAFAIFLLIR
                     QVNRMKRPAPAPAPSTKDCPFCLSAVPLKAIRCPHCTSELKSE"
     gene            11863..12057
                     /locus_tag="VMU21_09515"
     CDS             11863..12057
                     /locus_tag="VMU21_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HUO77799.1"
                     /translation="MKEFLSSKKFDFLAKDVVHDESARKELVEKYHRLATPTIVIGKR
                     VFVGFKEHRADIEKLLLSIS"
     gene            complement(12402..13826)
                     /locus_tag="VMU21_09520"
     CDS             complement(12402..13826)
                     /locus_tag="VMU21_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUO77800.1"
                     /translation="MKSLYPLLIGGTWEQSDEVMDVINPYNNQPITKVCRALPEHAER
                     ALHTAHKARPVMASLPSHQKAEILRKVAETIQANREELARTITLENGKPVQESRSEVD
                     RAALTFTIASEETSRIGGEFISLDRNKLSEGRWGITRRFPCGPVFGITPFNFPLNLVV
                     HKIAPAIAAGNPILLKPASKTPLSALMLGEIIMGSGIPKGCLSILPCGSKIAEKIITD
                     ERIKVLSFTGSASVGWQLKKLANEKRVLLELGGNAGVVIDRQCDLEYAIARCVTGSFS
                     YAGQVCISVQRVYVHKDIFDDFVENFTQKVRGLRMGDPLDDANHLGPMIDEENLARVE
                     AWVNEAKAAGAKVITGGKRKNNFYEPTVILDTTSQMKVSCMEVFAPVVTITKIDDFKD
                     GLNAVNESVYGLQAGVFTNNLKNAFLAFEVLEVGGVIVNDVPAYRVDHMPYGGVKMSG
                     FGREGLKYAIEEMTEIRLMALNIR"
     gene            14033..16441
                     /locus_tag="VMU21_09525"
     CDS             14033..16441
                     /locus_tag="VMU21_09525"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HUO77801.1"
                     /translation="MSRKIDLPVTGMTCASCAAAVEKSLRRIDGVRSAAVNFASEKAT
                     VELETPVDLNLLGEAIKAEGYGLLVGRIDFAVRGMTCAACVAAVEKALRGLYGVLKVT
                     VNLAAERASVEYLSTIVDFNDFKKVVGDAGYAAEQITGDFIDREKEQREREFLDIRKR
                     FLISAFLSALIFMGSMVRIPLLSEWPVLFVLATPVQLWAGMRFHRAAWAAFKHRTTNM
                     NTLITVGTFAAYLYSVLATFTPGLFLEGGIAPDVYYDTSAMIITLILLGRMLEAKAKG
                     RTSEAIKGLMRLQPRTARVLRDDGEREILTEEVMAGDIVIMRPGERIPVDGEITEGYS
                     TVDESMLTGESLPLEKSLGDRVFGGTINRSGSFKLRATRVGKGTALGQIIRLVEEAQG
                     SKAPIQRLADKVASIFVPVVISIAVATFVIWFVFGSKPAFTLSLMNFIAVLIIACPCA
                     LGLATPTAIMVGTGKGAEKGILIRDAEALELAHKVQAIVLDKTGTITKGEPEVVDIIP
                     VDGFAMDEVLRYAASAEKLSEHPLGQAIVRKAEEKGIGLSEPKEFLAIPGGGVRAVLD
                     GDSVIIGTLSLLQKEGVNVSSMRGTEEKVSSEGKTPVCVAINKKVGGMFAIADTTKDG
                     SADAIRELREMGIEVAMLTGDHKNTAEAIARIVRVDRVFAEVLPEQKVEVVKALKREG
                     KVTAMVGDGINDAPALAEADVGIAIGTGTDIAMEASDITLIKGDLRSVVEAIKLSKLT
                     LKTIRQNLFWAFFYNVVGIPVAAGLLYAFGGPLLNPMIASAAMAFSSVSVVSNSLRLR
                     RKKL"
     gene            16438..18012
                     /locus_tag="VMU21_09530"
     CDS             16438..18012
                     /locus_tag="VMU21_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HUO77802.1"
                     /translation="MKVVTTQEMREIDRRTIEEFGIPGQVLMERAGMAVASKIRELFA
                     QKKTIVLAGGGNNGGDGIVVARELFNSGWHVKVLLFAKQNKLSPDCLSQFRSAKQAGV
                     PVEIRAGITGGDLHSALVVDALFGTGLNKEVKGNLTEVISLLNRTDSPVLSVDIPSGI
                     SADSGQVMGVAVKADYTVTFGLPKRGHLLYPGAEYTGRLFTENIGFPEELLASDDLTV
                     ELLEEQYISQLIPERPRYSHKGDYGHVLIVAGSRGKTGAAFMAAKACMRAGAGLVTVG
                     VPESLADIVQSRVTEEMTLPLPDEGGGTLSSKASSVLLRFLTERADVLCIGPGIGVSN
                     ETRKLLAELITASRAPMVIDADGINSIDDVTLFGKAHSPVILTPHPGEMARLLNKKTS
                     PLARGSRKSGARELRSHIEQERIATGLSFSQETGAYLLLKGVPTVLAEPGGKAYINPT
                     GNPGMATGGTGDVLTGMVSSFMAQGLNPTDAAILGVYMHGLAGDIAADKKGEHSLIAS
                     DIIDSLPEVFKCLKGA"
     gene            18071..>18526
                     /locus_tag="VMU21_09535"
     CDS             18071..>18526
                     /locus_tag="VMU21_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HUO77803.1"
                     /translation="METLIYPEIFGKKVKAFFTGKVPGADFGRLAQLALLDKKNIYLP
                     IQKHTDKVIMLDSSLDPKIGDAVITSRKDILVGVQAADCVPILLYDRVSGVAGAVHAG
                     WRGTAAEILKKSIHTMMDRYHSSPKEVLVAIGPSIRWCCYHVGYEVIEAV"
BASE COUNT         4765 a         4412 c         4819 g         4530 t
ORIGIN      
        1 caaatcctgt gtacaatccc atacgggcac ctccttaagg ttaagattgg cttctcacct
       61 acgagcatac cgcaatacga tacgctcagg aggtgcccgc tatatgatta tcaaaccatc
      121 acctcgtcgg catcgttcgg cgcagacacc gttgtcttta ttgttgtccg atttcgcttt
      181 gttaatgttt ttgttgttag tccagagcgg gaattgatct tccagctgtt gcatgatcgc
      241 tgtttctctt tatctgcctt tattaattat ctgcgctctg gtggagcgaa acgacaccat
      301 tgcgctttgt ctgtgccaac gattactata caaactgtcg cattttccga aatctgtcta
      361 aactatagca taccgttagg atttttcaaa ttgaaaatca tacaaaaaaa tgcgtaaaat
      421 caattgatat tatgggtaaa aaacaaaatg gcggaaaatg ttctgacatt gtccgaaggg
      481 ttaagtggca ggcaactaca actaaggaat gaaaactgta tcttgaggaa ttccctccct
      541 atccctcaac aatccggtaa ctgaatgtga tggtggcgca gccctcgatg gttgctttta
      601 cagagcaata cttctccatg gagagattca cggccctctt taccgcttcc tcggagatgc
      661 tgtggcccgt caccacaaac tcaatatcga tagcggtgaa tttctttgga tgttcttctg
      721 cgcgtttgcc ctttatatga atctcaagtc cccgaatgtc ctgtttcttc ttcttaagta
      781 ccgagactac atccatgccc gagcagcccc ccagtccgac gaggaggagt tctgtgggtc
      841 tgacagcagt atttttccct ccgtactcct gttcaccgtc cattacgacg gcatggcctg
      901 actctgcttc tcccacaaac tggagaccgt caatatattt taccttaact tccattcaat
      961 tcctcctcgc gcagaaacgg gctatttacc cccccgaacc tgtaaaggtc accggggagt
     1021 ctttccctgt gaattatgga taagttaact gtaaccgctg gatgacggtt gattttccgg
     1081 tcttatcatc catctctatc accactgcgg aaagcatccc gttgccctta gctgtctcga
     1141 attttttggg catctgataa agaaacctct ctattatctg ttccttctca attccgatca
     1201 ccgagacttc aggccccgtc atccccacgt ctgtgatata ggcggtgccg ccaggaagta
     1261 tcttttcatc agcggtctgc acatgggtat gcgtgcctat cactgcgctc acttttccgt
     1321 cgacaaaata gccgaaggct atcttttctg aggtggcctc agcatggaaa tcaacgagga
     1381 taaacctggt ctcgttctgc atccttttga tctcgtccaa gccaaccctg aacggacaat
     1441 cgatgctggg cataaagacc cttcccgaga gattgagtac cgcgatcttg gttccgtgcg
     1501 gaaggggata gagaatactc ccgtagccgg gaacgcccgg aggataattg agaggtctga
     1561 taagacgatt ttcctttccg atataagaga ccgtttcctt cttgtcccaa atgtggttcc
     1621 cgcttgtaat cacatggatt ccgcagctga agagctcttc ggccaccttt tcggtgatac
     1681 caaaccctcc tgcggcattc tcgccgttcg caatgacaca atcaaccttg tagcggtcag
     1741 cgagggtcgg cacgagggct ttcaccacca tcctacccac cgtgcctacg atatcaccaa
     1801 tgaagagaac cctcatgcgg acaaatgtcc acccacgagg tatgcaccat taaaattcca
     1861 cgaaactcca aagcgggctt ggcgcgtcat aggcttattt tgcatattcc acgtatcgtg
     1921 actctcttat taccgttacc tttatctggc cgggataggt catctctgac tcgatctttt
     1981 ttgagaggtc cctggaaagc atggaagcca tctcatcggt cacatcctcg ggtttcacga
     2041 ttattctgac ttccctgccc gcctgaatgg catagcattt ttcgacacca ttataggaca
     2101 tcgccatctg ttccagcttt tcaagacgct ttaaatagtt ttcgatgctt tcccttctca
     2161 cccccggcct tgctgcagaa agtgcatctc cagctgcaac gagggcagac tccactgtgg
     2221 taggttcttc gtcgccatgg tgtgcgaaaa ttgcattgat taccttctgg ttttctccat
     2281 acttctttgc gatgttcgca ccaatttcct ggtgggagcc ctccatttcg tggtccaccg
     2341 ccttccctat atcatggaga aggccccccc tcttcgccag cttgatgtca actttgagtt
     2401 ccgcagccat ggtccctgca aggtatgcaa cttccctgga atgctggagc atgttctgcc
     2461 catatgaggt cctgtatttc agccttccga caagcttgat gatttcggga tgtatgccgg
     2521 agagaccgag gtcaaagacc gctttgtccc cttcttcctt gatggtggca tccacttctt
     2581 ttttgacctt ttccacgatc tcttcgatcc tcgtgggatg tatcctgccg tcagcaatca
     2641 gcctttccag agaaagacgc gcaatttccc tcctcaccgg atcaaaggcg gagagcgtca
     2701 caagttcggg agtgtcatcc acgatgagat caacaccggt agccgcctca agggcacgaa
     2761 tgtttctccc ctctcttccg atgattctcc ccttcatctc gtcgttgggg agactcaccg
     2821 cagttacggt tccatcagca acataatcac ttgcataccg ttggatggtg aggctcacaa
     2881 tctcctttgc tttcttttct gcctgctccc ttgcttcatc ctcaagcctt tttgcaagtt
     2941 ttgaggcctc aaaacgtgtc tcctcctcca ccctcctcag gaattcctgt ctggcgtctt
     3001 cggtgctcag tcccgacacc ctttccagga ccaccatctg ctccttgata atctggtggt
     3061 agtggttttc cttatcctgg agtccgcgtt cttttgagtt gtattccttt tccctcttgt
     3121 taagttcatg ctcccttttc tccgcctgac ttaccctccg ctccaatgtt tcttccttct
     3181 gcctgagcct ccggtccagc tgattcagtt cgtgccggcg ctccctcagc tctttttctg
     3241 catctgactt tgcctggtat acgacatctt ttgcctctac tgcagcttct ttttttacta
     3301 cgtccgctgc cttctttgcc tctgcaatga tgtcttcgcg ctcttttttc agggctgatt
     3361 gctgggaccg gagagaacgt ttgtatatga ggttcaggag aagtccgagt gaaatgccga
     3421 tgagaacggt aatgagcaga tacaatgcat caaacatcat tattattccc cctttcgtaa
     3481 ctgcgttcat gagggagctg cgggcttatg acgtgggctt gtcagtgaga ggatagtacg
     3541 tgggactgtg atgtgttcaa atcgagacct ctatgctgaa caatcacgat cttcacagtc
     3601 gcgtaaagta atgtttctat cgttgaagat agccacagta ccagaatctc tatcctttta
     3661 tctatattct ccacaaaaaa caaagctgtc ataattatcc gcaatataaa cccgccctgc
     3721 cgtgcaagtg aataatcaga tccgcctata atataggtgg gtgtcttgaa ggcgacatca
     3781 ggcttccgaa cacgtgagtg ctcggcatgc acaccgtacc ccttacgcag actcccaaaa
     3841 gatctaattt gccggatcaa cttttttcac ccatcacgaa cagggcaggc taactgccct
     3901 caatcttatc ttaattatat ttataacaga aatggagggg ttagagcaag gggaatttat
     3961 tgcttataga agaaatgaaa acccagggaa taaggatctt ttggttccca tgaaggccat
     4021 acccgcgtcc ggtgacagaa aacgggagaa tactacacaa tgagaatccc tgcgtacccc
     4081 accacatggt cataatcgcc gctcacgtct cctgatgttg catagcggac aagccttgcc
     4141 tcccgggcac cgagggattt caccgcaaag agcataatgg ttgcggggag gtacccgcac
     4201 atggaaatct gctcttttct cacgacggcg aagagacctt ccggatccag ggaaaggatt
     4261 ttatcaattg ccattttatc ctttttccgt gccaccccgt ctgatacata gtggctcata
     4321 tcggaactag ccacaattac gacaggataa tcggtctctt taatggactt tgcgagtccc
     4381 tcgcccaagt tcttgcactc atcaatggtc gctgacatga tggtcagggg tacaattttc
     4441 agggttttcg aaaagtgggc aataaaaggg agctgaacct caagggagtg ttcaaacata
     4501 tgggcctggc tgtcacccga tacaaaaggg gaattggaaa ggatctttct tccgagttgt
     4561 tcatcgatct ggaatattcc catgggaatt tcccactcac cctggggcat taaggacatg
     4621 cgtgcaccaa ggccggtgtg gtttggaccg agtaataaga atgtttccgg aatgatgatc
     4681 cttgaataca cctctccagc cactgcgcct gaatagacga gacctgcatg gggggagaga
     4741 attgcaaggg catgttcctt gggaagatca tccgccacat actgccggac ctgatggctt
     4801 agtctcgttg aggattcgtg ataaaattgt cctgcaaccg ctggttttct cctcatatat
     4861 actccgcagg ggtgattatc aaaaaacacc tttattaaat attatcaggt aaaagaatgg
     4921 aatgcaatta ttgtgagctg gggagacaga tatgcatcaa catgtcaggg gggttcccag
     4981 gataagcgaa tttacaacca tgcattgggt cataaaccgg caggcagtgt aaccctcccc
     5041 atgggtggaa atcaatcgct ttatgacaaa aaacggcaga tgatcggtct catccattgg
     5101 ccattaactc gtaaggccct gcaacaaaaa acatttcttg ggcattgcca aatgaaaagc
     5161 tggtacataa cttgctgctt aatactcgcg gaggtgatca ccatggcaat gtatcggacc
     5221 aaaaagcttg tctgtgtgtt gttggaatcg ctgctgtata agaagttgtc tctgaaagag
     5281 aaagaatcac ttcttatgaa agtggcggag aattacccgt tcccggcaga ggccgaagag
     5341 gaaagggcaa tagggtatga atcaagctgg aagggagtta tccatttgca ttaggaacat
     5401 aaacagcaga aaccttgggt tgacccctgg agaacgaact cacgcacaaa tgggcgcgaa
     5461 gcccaggagg tgaggaatag tcgcccttac tgagcgatgg gattacctaa cgccacgggc
     5521 aggggcatga aaacgagcga atacgtatac cgctcacccc aagggtgtca atggtattcc
     5581 gcatgcgtta aggtttcgcc cctttcccct gtcgcatcct tcttgatttt ccagtgccgt
     5641 agtgaggtga aaggtttttg ggttgaactt gtgtgtccgc atgggttaat atttttgtat
     5701 gcaccaacat tcggattacg ttccgctcca tctccacaca gaatacagcc tgctcgacgg
     5761 ttccattaag attgatgagc ttgcggagct cgcctcccgt tataagatgc cggcagttgc
     5821 catgacagac cacgggaacc tcttcggggc gatagagttt tataagaaga tatcgaaggc
     5881 aggaataaag cctattatag gctgtgaagt ctacgtggct ccctccggcc atctcgagaa
     5941 aaagggggtc acgggtgggg agactccctt tcaccttatc ctcctcgcac gggacaatgc
     6001 agggtacaga aaccttgtaa ccctcgtgtc aaaggcatat accgaggggt tttattataa
     6061 acctaggata gataaggatt tgctcgcaca gtacagtgga ggacttatcg cactgtctgc
     6121 atgtctcaaa ggtgaggtac cgtcctatgt gaacaaggac atggtggata aggcaagaca
     6181 gtgcgccctt gagtacaaac acatccttgg ctctgagaat ttttatatcg agatacagga
     6241 taatggcctc aaggttcagg aaaaggcaaa caaaaagctc attgaactcg cgcgtgaact
     6301 tcatgtcggt atcgtcgcaa ccaatgactg ccactatctg aggaaagaag atgcccgtgc
     6361 acacgatatc ctcctttgca tccagacagg caagacagag agcgacagta acaggatgcg
     6421 ttttgaatcc aatgaattct atttcaagtc gcccgaggaa atgaaaaggg ctttcagtga
     6481 actccccgat gcgctgttgt ccacaagaga gatagccgaa cggtgcaatg tcgaatttac
     6541 cctgggaaag agtttgctcc cgatgtatac gcgggaagat ggggtatctc ccgaagtatt
     6601 cctcaaacaa ctggccgcag aaggtctgag gaagaagttt ggcaataatc ctcccgattc
     6661 atacagaacg agatttagaa cggaactgga tattatcaac aggatgaact atgcgtccta
     6721 ttttcttatt gtgtgggact tcataagtta tgcaagggaa aatagaatac cggtgggtcc
     6781 gggaagaggt tctgttgcgg ggagccttgt tgcatactgt cttggaatca ccgagataga
     6841 tcccctgcgt tacaatctcc tttttgaaag gttcctcaat cctgagaggg tgagtatgcc
     6901 ggatatagat gttgattttt gcaaggacag aagaggtgag gtaatctcgt acgtgtcagg
     6961 gaaatacggc aatgaccatg tggcgcagat tatcaccttc gggacgatgg ctgcaaaggc
     7021 agccatcagg gatgtgggga ggtccctgga tgttccctac gcggaggttg ataggatcgc
     7081 aaaacttatc cccaatacac tgaatatcag tattgacgag gcgctgcggg gagagccgca
     7141 actgaaggca ttatacgaga gtaatgcaaa ggtgaaggaa attcttgata tcgcaaagag
     7201 actggagggg ctctgcagac acgcctcaac ccatgctgca ggcgtagtag tatctccgga
     7261 acctctcacc gagtatgcgc ccctctataa gaatcccact gatggtacca tcacgaccca
     7321 attcgatatg ggttcagtcg aggccatcgg cctcctgaaa tttgatttcc ttggtctcaa
     7381 gaccctcacc gttatcgaaa agacgcttgg gtatctcaag gcggggggca gggaaatctc
     7441 cctccacgaa attacccttg aggatcccga gacatatcgg ctcctcagtt ccggtcagac
     7501 caccggggtc ttccagcttg agagcccggg tatgcgggat atcctcataa ggatgcagcc
     7561 aaaccggttc gaggacctca tagcacttgt ggccctctat cggcccggcc ccatggcatg
     7621 gattgacgac tttatcaagc gaaagaaagg cgaggcagcc atctcctata aaatgccgca
     7681 gttgaaagag ttccttgatg agacctacgg gatcatcctt taccaggaac aggtgatgat
     7741 gattgccaac aagattgcaa agttctctat gggacaggcg gatatcctga gaagggccat
     7801 gggaaaaaag aagcctgagg aaatggagaa acagaaagaa agcttcatta agggtgcggt
     7861 gggcaatgga atcacggaaa agaaggcagc gcagctcttt gaaactatgg ctcccttcgc
     7921 aatgtatgga tttaataaat cccattctgc ggcgtatgcg tatattgcgt atcagaccgc
     7981 ctatttgaag gctcactatc cggtggaatt tatggcggcg acattaagta ctgatacgga
     8041 caacaccgat aagatcgtga agtcgatcgc ggaatgcagg aagatgtcta tagagattct
     8101 gcccccggat atcaatgaga gcaaccggga atttaaggta atcggaaagt ccatacgctt
     8161 tggcctcgaa gcggtaaagg gagtgggctc ttccgcaatt gagaccatac ttgcggcgcg
     8221 gatggaggac ggacctttta aaaccatgga ggattttctc cgcagggtgg atggcaagaa
     8281 ggtgaacaag aaggtaattg agagccttat taaggcagga gcatttgatt cctttggtat
     8341 gcaaagggca aaagcacttg aaatcctcag caccgtactt aacagtgata cctcggtgag
     8401 cagcctgttt gctcagcagg atatttttga tgaagggagt gaaagacaag gaaaagagtg
     8461 ggatgagatg gagctcctcc gctatgaaaa ggaagcactc ggcttttata tcactggaca
     8521 tcccctctcc aggtaccggg aatccctctc cgcaatgaag gtgagggaaa tctcacatct
     8581 tgaggatgtc gccgataaag aggaagtgca gatcgcgggt gtcataagca gcgttaagaa
     8641 aatcaaaaca aaaggaaagg ccgaaattat ggcttacctg actatagagg atgaagaggg
     8701 gagcatggag gctatcgtgt ttccggatct ctacaggacc atgctggata tgttcagtaa
     8761 agagagcgtg attgttataa agggtaccat cgataagact gagaaaggaa gtaagcttat
     8821 cacaagagaa gcgtataggc tcgatgactt cttaaatgct gtgccattgc ggttatccaa
     8881 acattctcct acggcgcaaa atggtatgca gaggatggag ataaccctcg cggcccatcc
     8941 cgcggccagg gaaggcctca ggacgctcaa ggaacttata tccgactatc cgggaagatg
     9001 ccccctctcc ctcaggatag agttgaacgg ctcctatgcc ctgatggata ccgcgcttca
     9061 cgtggaacca tcttctgtgt tggtcgaacg gattgaaggg ctcatgggaa agggtgcggt
     9121 cagaatacgc taggaacgga ccgtacagca caatgaaagc ctcacatcac caaggcctga
     9181 cacgaagggg agattttgtt ccatgaaata ttatctggat tttgaaaaac ctatcgagga
     9241 acttgagaca aagattgagg aactgaaacg cctctctgac ggtaaggata ttgatattac
     9301 ctcagaggta aggaaactgg aaagaaaagc gaaagacctg cgttccgaaa tattttccca
     9361 tctcactccc tggcaaaaga ctctggttgc gagacaccct gacaggcctt ataccctcga
     9421 ttacatcgac cttttgtcag aggactttat tgaactccac ggagacaggc gatttgctga
     9481 tgacccttcc gtggtgggag ggtttgggag gatcagaaat accacgttta tgattgtagg
     9541 acatcagaag ggcaggggca cgaaagaaag gattctgagg aatttcggtc agccacaccc
     9601 ggagggatat aggaaggcat tgcgacttat gaaacttgca gaacgattta agagaccggt
     9661 attgaccttt attgacaccc cgggggcata tcccggaata ggtgcggaag agcggggtca
     9721 ggcagaggca atttccacaa accttatgga aatgtcgaga ctcaagaccc cgattatctc
     9781 cgtgttgatt ggggaaggag gaagcggggg tggcatcgcc ctgggggttg cagatagaat
     9841 ctatatgctt gaatattcgg tatattcagt gatatccccc gaaggctgtg ccgccatttt
     9901 gtggagaaag agcggagacc tgggtactgc ggagttcaca aaagcggcgg atgcgcttaa
     9961 gctcactgcc caggaccttt tcagatttaa ggtaatagat gaggtgattc cggaatcctc
    10021 cggtggcgcc caccgggacc cttccgggac agcggagaag gtagcggata tggtgatgaa
    10081 ggctctggaa gagctgaaga caaaaacgcc cgccaggatt gtggaagaga ggtatagacg
    10141 gttaagaaag attggagtgt tcgaagaaac agcagctgct tcttcaaaca agggggccta
    10201 atgaaaggta cgctgatatt tctcgttttt gtccttgcag tgcttgcatt cctctcaatg
    10261 atttcgggga ggcgggttcc cccgccattt attccacaag acagtcagca catcgttctt
    10321 gacgatccca aagcctgtct ggaatgtcat ggacccggta agggagcgcc attaaagaaa
    10381 gctcatcctc cgaagagaga gtgtctgaaa tgccataaag tgaagggcga aggcaaaagt
    10441 aattaaagag acgaaaagtg agaagtcctt gactcctggg acaaccagcc atgatacgag
    10501 aggaggtaag gcagccacct gcgcaatttg cggaaagcag atggagagcc ggaaaacctt
    10561 tgtatgcaag aggtgcagaa agtcacctct ctgtttcgaa catatggaca gagagtacaa
    10621 actatgttcg gcatgcgctg ccgaggagcg cttaacgctc tatcaaaatc ttgccaccca
    10681 ggaaaggaat gtgaaaagtt ttttgaggct tatgcagttt attcttacct tggtcgttat
    10741 attttatggc gtaaagagat ttttctatga gctggtaccc gatcccttga aggtgaatgt
    10801 gttctttgac tatctcttcc tctggggagc aatctcattg gccggcatgg cactctctta
    10861 cgtactactg atttctcagg gacagaagat cagggaaata gaggataaga ttcgggatca
    10921 caaagcatat accaaatacc tccggcgctg aatcaacaag aacccttcgc cgcccaccaa
    10981 tatccaggtg atgggtgtgt acccacccgg aagacaaagg tattttccaa gaggcccgca
    11041 cttatttgaa ttgagctgaa tccagtgatc ccctgtaagc aaccatgcga tctgtaccac
    11101 ttcccgggga gatgaagacc ttctcttttt tcatgccttt ttccctttat tctctggcag
    11161 tttgatactc tattcagatt tcagttcaga ggtgcagtgc gggcaacgta tagccttgag
    11221 gggaaccgcc gaaagacaaa aaggacaatc ctttgtcgaa ggcgctggtg ccggcgcggg
    11281 ccgcttcatc cggttcacct gacggataag caggaagatc gcaaaggcaa tgatgataaa
    11341 gtccagcgtt gtgttgaaaa aaatgccgta attgagggtt gctgcacccg cggcctttgc
    11401 cgctgccact gtcggatagt gctgtccgga taaattgatg aagagattcg aaaaatcaac
    11461 ctttccaagc ataagcccta tggtcggcat gaggatatca tttacgaacg aggtgacaat
    11521 cttcccaaag gctgcaccga tgataattcc caccgccatg tccaccacgt ttcccctcat
    11581 tataaattcc ttaaactctt ttaacatacc tagtcctcct ttccgggtct ttaacaagtc
    11641 ccgcagcatg tcactataac ctcattatac cgcatgtttc cccgccagga taggggaaca
    11701 ctcagtgtac gtctatgcag ggatcttgac accggaccac tttgccccta atctatggga
    11761 caaagacata ttggtatcaa caggaaataa ggaccacaag aaagagggag aagagtggtg
    11821 ataactattt acacgtcgcc cacatgagct gaatgcaatg cactgaagga gtttctttca
    11881 tcaaaaaagt ttgactttct cgcaaaggat gttgtccatg atgaatcggc aagaaaggaa
    11941 ttggtcgaaa agtaccacag gctcgcaaca cccacgatag tgatagggaa gagagtattc
    12001 gtgggcttta aggagcacag ggcggatatt gaaaaactgc tgttaagcat ttcatgacag
    12061 gggaagaaga aaggaccttg ccagggagac gattggacac tttcttgcct aaattgcata
    12121 agaagcggtc tcttgcctct ctatgggcat aaggagagca gagaccgagt gaaaagcccg
    12181 ggagtgtctt cacgataccg tggtgcggta atcagcaaga gcccttgcac ttacaggttg
    12241 ttgccatttt ctttgagacc ttcttaatct tcacagaatc accaccttta taaaagacca
    12301 gatttaaata cacacacctc gggcaagaaa aatcatatcc gctttccccc ttgatgtcaa
    12361 gcgagggaaa tattaggggg tcctcacctc acaagggaaa ctcatcgtat gttcagggcc
    12421 atgagccgta tctcggtcat ctcctcaatg gcatatttca ggccttccct cccgaatccc
    12481 gacatcttta cccctccata gggcatatgg tcaaccctgt atgccggcac atcattgacg
    12541 atgacaccgc ccacctcaag aacctcgaag gcgaggaagg cattcttcag gttattggtg
    12601 aagacccctg cctgaagccc gtagaccgat tcgttcaccg cattcaggcc gtccttgaaa
    12661 tcatctattt tcgttatggt caccacaggt gcgaagacct ccatgcagct caccttcatc
    12721 tgggaagtag tatccaggat aacggtgggc tcataaaaat tgtttttcct cttccccccg
    12781 gtgatgactt tcgcaccagc agcctttgcc tcattcaccc atgcctccac ccgtgcaagg
    12841 ttctcttcat cgatcatggg accaaggtgg ttagcatcat cgaggggatc acccattctg
    12901 agccccctca ctttttgggt gaagttttcg acgaaatcgt cgaaaatgtc tttgtgcaca
    12961 taaaccctct gcacagagat gcatacctgg cctgcatatg aaaatgagcc ggtcacacac
    13021 cgggctattg catattcgag atcacattgt ctgtcaatga caacacctgc gttccctccc
    13081 aattccagga ggaccctctt ttcgttggcc agctttttca gttgccagcc caccgatgca
    13141 ctaccggtaa aactgaggac cttgatcctc tcgtctgtga tgatcttttc cgcaatctta
    13201 ctgccgcacg gcaagatact cagacagcct ttggggattc cggaacccat gatgatttca
    13261 cccaacataa gggcagagag gggcgttttt gaggccggtt taagaagtat gggattccct
    13321 gcagcgatgg caggagcaat cttgtggact accagattca aagggaaatt aaaaggggtg
    13381 atgccgaaca ccgggccgca ggggaatctc ctggtgatgc cccatcgtcc ctctgacaat
    13441 ttgttcctgt caagactgat gaattctccc cctatccggg atgtttcctc agaggcaatt
    13501 gtgaaggtga gtgcggccct gtccacctcg cttcttgact cctggaccgg tttgccgttt
    13561 tccagggtga tggttcgcgc gagttcctcc ctgtttgctt gaatggtttc ggccaccttg
    13621 cgcaggatct cagccttctg gtgcgagggg agggatgcca tgacaggacg ggccttgtgc
    13681 gcagtatgta atgccctctc agcatgttca ggcaatgccc tgcagacttt ggtaattggt
    13741 tgattgttat agggattgat gacatccatg acctcgtcac tctgttccca tgttcccccg
    13801 ataagcagcg gatataatga tttcatcgtg cacctcctcc ttcactttta gcaaaaagca
    13861 agaataagcg caagaggctc tcccggatgg ggaagcgtac gttgcaacgg taagaagaac
    13921 ccagcactcc acataaacgg tctgtaccag gcagaagcag ccgtttttga tataatgaag
    13981 aaggtactga tcacgcacac cagaagggaa aacgaaggat gaagggatgg gaatgagcag
    14041 gaaaattgac ctgccggtga ccgggatgac ttgtgcatca tgtgcagcgg ctgtcgaaaa
    14101 aagcctgagg agaattgacg gggtgaggag tgccgcagta aattttgcct ctgaaaaggc
    14161 cactgtggaa ctggaaactc ccgtggacct gaaccttctg ggggaggcca taaaggcaga
    14221 ggggtacggg cttcttgtgg gcagaataga ttttgcggtg agggggatga cctgtgccgc
    14281 gtgcgtcgct gcagtggaaa aggcactgag ggggctttac ggcgttctta aagtgacggt
    14341 aaatcttgcg gccgagaggg cttcagttga atacctgtcc actattgtgg atttcaatga
    14401 tttcaagaag gtcgtggggg acgcgggata tgccgcagag cagatcaccg gggattttat
    14461 tgacagggag aaggaacaga gagaaaggga gttccttgat atcagaaagc ggtttctgat
    14521 cagcgcattc ctttccgcgc tcattttcat gggaagtatg gtgaggattc ccctcctttc
    14581 agaatggccg gtactctttg tgctcgcaac gcctgtgcag ctctgggctg gcatgagatt
    14641 tcacagagca gcctgggctg cctttaaaca tagaaccacg aacatgaaca ccctgattac
    14701 ggtggggacc ttcgctgcat acctttatag tgtcctcgcc accttcacgc ctggcctctt
    14761 tctggagggt ggcattgctc ctgacgtcta ttacgatacc tcagccatga tcataaccct
    14821 catactcctc ggaaggatgc tggaggccaa ggccaaggga aggacttcgg aagcgatcaa
    14881 ggggcttatg aggctccagc ccaggactgc gagggtattg agggatgacg gagagaggga
    14941 aatccttact gaagaggtta tggcagggga tatcgtgatc atgaggcccg gagaaaggat
    15001 accggttgac ggagagatca cggagggata ttcaacagtg gatgagtcca tgcttacggg
    15061 tgaaagtctt cccttggaga agagcctggg ggacagggtc tttggaggga caataaacag
    15121 atcgggaagt ttcaaactca gggccacgag ggtgggaaaa gggaccgccc tcggccagat
    15181 cataaggctt gtggaggagg cgcaggggag caaggcgccc attcagcgtc ttgcagacaa
    15241 agtggcatcc atattcgtgc ccgtggtaat ttctatcgcg gttgcgacat tcgtgatatg
    15301 gtttgtattt ggatcaaagc cggcatttac gctaagcctc atgaacttta tcgcggtcct
    15361 cattatcgcc tgcccatgcg cccttgggct tgcaacacca accgccataa tggtgggcac
    15421 agggaagggc gcggagaagg ggatactgat ccgggatgcg gaagcattgg aactagcgca
    15481 taaagtgcag gccattgtgc tcgataaaac cggcaccatc accaagggag agccggaggt
    15541 tgtggatatc atacccgttg acggctttgc catggatgaa gtattgaggt atgctgcgtc
    15601 agcggagaag ctttcggaac atccgttggg tcaggcgata gtcaggaagg ctgaagaaaa
    15661 agggataggc ttaagtgagc cgaaggaatt tctggcaata ccgggcggag gcgtaagggc
    15721 ggtattagat ggcgattcag taatcattgg cactctcagt ctattgcaaa aggaaggcgt
    15781 gaatgtgtcc tcgatgagag ggacagaaga gaaagtatcc tctgagggga agacacctgt
    15841 gtgtgtggcg attaacaaaa aagtgggggg gatgtttgcc attgccgaca caacaaagga
    15901 cggctccgct gacgcaataa gggaactgag ggaaatgggg atcgaggtgg caatgctcac
    15961 cggagaccat aagaataccg cagaggccat tgcgaggatt gtgagagtgg acagggtttt
    16021 tgcggaggtc ctgcctgagc agaaggtgga ggtggtgaag gcgttgaaga gggagggcaa
    16081 ggtcaccgcc atggtgggag acgggatcaa tgatgcccct gcacttgccg aggctgatgt
    16141 gggaattgcc ataggcaccg ggacagacat cgcaatggag gcgtctgata tcacccttat
    16201 caaaggagat ctgaggagtg tggtggaggc cattaaactc agcaaactca ccttgaagac
    16261 catcaggcag aatctctttt gggcattttt ctacaacgtg gtggggatcc ctgttgccgc
    16321 gggacttctc tatgcattcg gcggacccct tttgaaccct atgatcgcct ctgctgccat
    16381 ggcattcagc tcggtatccg tagtgagtaa ctcgcttaga ttgaggagga agaaactatg
    16441 aaggtcgtca ccacacagga aatgagggag atagacagaa ggacgattga ggaattcggt
    16501 attcccggac aggtcctgat ggagagggcc ggcatggcag tggcatctaa gatcagggaa
    16561 ctttttgcgc agaaaaaaac catagtcctt gccggcgggg gaaacaacgg aggtgacggg
    16621 attgttgttg cacgtgaact gtttaattca ggatggcatg tgaaggtctt actctttgca
    16681 aaacagaaca aattaagccc tgactgcctt tcccagttca ggagtgcgaa acaggcgggc
    16741 gttccggtcg aaatcagggc ggggataaca ggaggagacc tccacagcgc gctggtggtg
    16801 gacgcactgt ttgggacagg actgaacaag gaggtaaaag ggaacctgac agaggtcatt
    16861 tcattactga acaggaccga ttctcctgtg ctgagtgtgg acataccctc aggcatatcc
    16921 gctgattccg gccaggtgat gggcgtggcg gtgaaggcgg attataccgt aacattcgga
    16981 cttcccaaga ggggccatct cctctatccc ggagcagagt acacaggaag gcttttcaca
    17041 gaaaacatag gattcccgga agaactcctt gcttcggacg acctgaccgt ggaacttctc
    17101 gaggagcagt atatttccca actgattcct gaaagaccga ggtattccca taagggagat
    17161 tacggtcatg tactcattgt tgctggttcg agaggcaaga cgggcgctgc gttcatggcc
    17221 gcaaaggcat gcatgagagc gggtgcaggg ctggtcacgg tgggtgttcc ggagtccctg
    17281 gcagacattg tgcaatcaag ggtcacggaa gagatgacac tgcctttgcc cgatgaggga
    17341 ggcgggaccc tttcgtcaaa ggcttcatcg gtacttctca ggttccttac agaaagggcc
    17401 gatgtgcttt gcataggtcc ggggattggg gtgtctaatg aaaccaggaa gttgctggca
    17461 gaactcatca ccgcttcaag ggcgcccatg gtgatcgatg ctgacgggat aaactccata
    17521 gatgacgtaa cacttttcgg gaaggcccat tcgcccgtga tcctgacacc ccatccaggt
    17581 gagatggcaa gactcctcaa taaaaaaaca tccccattag ccaggggaag ccggaagtcc
    17641 ggagcaaggg aactcaggtc tcatatcgag caggagagga ttgccactgg cctttcattc
    17701 tcacaggaga caggggccta tcttctgctc aagggtgttc ctacagtatt ggcagagcct
    17761 ggaggaaagg catacattaa tcctacagga aatccaggca tggctacagg ggggaccggt
    17821 gatgtactca ccggtatggt ctcttccttc atggcccagg gcctgaaccc gacagatgct
    17881 gcgatacttg gggtgtatat gcacggactt gccggtgaca ttgctgctga taaaaagggc
    17941 gaacattccc tcatcgcatc cgatatcatc gattctctgc ccgaggtctt caaatgtctg
    18001 aaaggtgcat agcccggatc cgggatgtat cctcatgcga ggaatcctgg tctgttctca
    18061 cataggatga atggaaaccc tgatctatcc tgagatattc gggaagaagg taaaggcatt
    18121 ctttacagga aaagtaccgg gggctgattt tggccgactg gcacaactcg cattattgga
    18181 caaaaagaat atctatttac caattcagaa acataccgat aaagtgatca tgcttgattc
    18241 ctcccttgac cccaaaatag gggatgcggt gattacaagc cggaaagata tccttgtggg
    18301 agtgcaggcc gcggactgcg tgcccatcct cttgtatgac agggtttcag gtgtggcagg
    18361 ggccgtacac gcaggatgga ggggaacagc agcggaaata cttaaaaaga gcatacacac
    18421 aatgatggac cgttatcatt catcgccaaa agaagttctg gttgccatag gaccgagtat
    18481 acgatggtgc tgttatcatg tggggtatga ggttatcgag gcagtg
//
LOCUS       DATKOU010000205         3216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1453920, whole genome shotgun sequence.
ACCESSION   DATKOU010000205 DATKOU010000000
VERSION     DATKOU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453920"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..612
                     /locus_tag="VMU21_09540"
     CDS             304..612
                     /locus_tag="VMU21_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77804.1"
                     /translation="MRARAIRTTVVVLLLFLFTLNCAKPQTMSSWDRHCVACHDGETV
                     LNGMVLMNREGMKAKYKNLEEFVNACEGSGACMNILKHQKKLLTEVGKELGISDSSRK
                     "
     gene            complement(620..955)
                     /locus_tag="VMU21_09545"
     CDS             complement(620..955)
                     /locus_tag="VMU21_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77805.1"
                     /translation="MERERLVHDLIVDRLRGKFSKEYREIEVNPEGSPDIVLASHGLT
                     MAIVEVETDGSITPDKAGKWKEMVQSGSKLILMVPKNAKVKTTELLWKHGIADRVGVG
                     SYEIVITMP"
     gene            1257..1553
                     /locus_tag="VMU21_09550"
     CDS             1257..1553
                     /locus_tag="VMU21_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HUO77806.1"
                     /translation="MDEDEMAREFEPVDLPIDGTLDLHTFHPREVKDLLPHYITLCRE
                     RGILQVRVIHGKGTGALRETVHSLLSRLPEVSSFGLAGEDGGGWGATIVMLKPL"
     gene            complement(1605..1952)
                     /locus_tag="VMU21_09555"
     CDS             complement(1605..1952)
                     /locus_tag="VMU21_09555"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HUO77807.1"
                     /translation="MKEQSTMNFRHPRISREFKTIRAMITLYCHDHHATGRQLCSECR
                     ALLDYAGLRLEKCPFQENKTTCANCAVHCYTRSMREKVKEVMRYSGPRMTYRHPLLAF
                     YHLIDGRKKASAP"
     gene            complement(2083..3129)
                     /locus_tag="VMU21_09560"
     CDS             complement(2083..3129)
                     /locus_tag="VMU21_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77808.1"
                     /translation="MARIVFERIQSGVRFHNPLRDFAEDLHMLEIRKDPLLGDVSIHN
                     PYLKDKAKAFFVECDQDLIRRLVEESGKNCFFCGEGVEKGTPRFTSDFVAEGRLRVGE
                     AVLFPNLFSLGTYHPVVCLCKAHFLKLSEFRPDLLADGLTAAQEFLKIVYQKDPSASF
                     TAVTANYLFPAGASLVHPHLQMLITPVPYSYHRRLIEAGNSYFREHGSSYFTDLIDQE
                     REGSRYVARQGRWNWLVPFSPMGNNEVMAVHDSVPDYGLLSEVDVTDLAHGISRVLSF
                     YETLGHLSFNYAAYSVRDHRAGKGFRCIFKIITRQNLYPNYRNDDYFLQKLLQTELII
                     TPPEELAMALKGHF"
BASE COUNT          874 a          774 c          767 g          801 t
ORIGIN      
        1 cccttcctta aagaggtaaa tgaatagaat aagaatgaga tatgtgttca gaaccgctcc
       61 tcctttattg accccgtgat tttattttag cctaaatcct cctcacacgt cttttgtaaa
      121 tatagaacgt agaccctgat aattgcagtt aagaccagta ccagttacag agttacgaag
      181 aaatgttctc tataaaaccc ctgctctgct tcttataatt attcttgttg cccaaccaga
      241 atctgatata ctataaaaat agctaaggaa gctgattatg tctcgtaaac gaagagctaa
      301 caaatgaggg caagggcaat aagaactact gtggtggtgc ttcttttatt cttgttcaca
      361 ctgaactgcg ccaagcccca gaccatgagt tcgtgggata gacattgcgt ggcatgccat
      421 gatggggaaa ccgtactcaa tggaatggtc ctcatgaaca gagaaggaat gaaagcaaag
      481 tacaagaacc tcgaagaatt cgtgaatgcg tgtgaaggct ccggggcatg catgaatatc
      541 ctgaaacacc agaagaaatt attgacagag gtggggaagg aactggggat aagcgatagt
      601 tcacgaaaat gactggattt tacggcatgg tgatcactat ttcgtaactc cccacaccta
      661 ccctgtccgc aattccgtgt ttccacaaaa gttcggtggt cttcaccttt gcattctttg
      721 gaaccatgag gatcagcttc gagccggatt gaaccatctc cttccacttc cccgccttat
      781 ccggggttat gctcccatcc gtctccacct ccactatggc catggtgagg ccgtggctgg
      841 cgaggacgat gtcgggactt ccttccggat tcacctctat ttccctgtac tcctttgaaa
      901 acttccctct gaggcgatca acaataagat catgcacaag tctctccctt tccataaacc
      961 ctcctttcgg ataatcccag tttttgagca acggcaacta ttacgtggtg taaaaaacct
     1021 cggaaaactt cagcactatt atattccctc gctaaccagg aggcaagtaa gggtaatgaa
     1081 atctcgctcc ttcgcctcac tgagccttgt cacgtgttct tgacttgtgg atcaaaaaag
     1141 cctaccttta acaaaggaaa gaagttgaaa aagaaaaccc tgtcattatg gcgaaaatag
     1201 agtgttgaag cagtattcat cctcgaaagg ggtacaggta acactcggga gtattaatgg
     1261 atgaagatga gatggcaaga gaatttgaac ctgtagatct ccccattgac gggactctgg
     1321 acctccatac ctttcatccc cgggaggtga aagatctcct gccccactac ataaccttgt
     1381 gcagggaaag ggggatactc caggtcagag tcatccacgg gaagggtacc ggggcgctca
     1441 gagagaccgt acattccctt cttagccgtc tgcccgaagt ctcctccttc ggactggcag
     1501 gtgaagatgg aggaggctgg ggagctacga ttgttatgtt aaaaccatta taaacgatac
     1561 ccgatagatg aaacaaccaa gcaggaacgc atttggcgct tgtcttacgg ggcggacgct
     1621 tttttgcggc catcaataag gtgataaaaa gccagtaacg gatgacggta ggtcatgcga
     1681 ggacctgagt accgcatcac ctctttaacc ttctccctca tggaacgggt ataacaatgc
     1741 accgcacaat tggcgcatgt tgtcttgttc tcctgaaagg gacacttttc gagccgaagc
     1801 cctgcataat ccaggagtgc cctgcactca gaacagagct gtctgcccgt cgcatgatgg
     1861 tcatgacaat agagcgttat catggcccgg atcgtcttaa attctctgga gattctggga
     1921 tgtctgaagt tcatcgtgga ttgttccttc actatctgtg gtatctcatt ctttttttga
     1981 tatacactgg agtattgtct tcacggtact agaatgacag gaataatcgt accctcacaa
     2041 tgatttaaat cagggctatt cccccctcac agcaaatacc atttagaaat ggcctttcag
     2101 tgccatagcc agctcttctg gcggggttat gatcagttct gtttggagaa gtttctggag
     2161 aaaatagtca tcattccggt aattgggata aaggttctgt ctggtgatga tcttgaagat
     2221 gcatcgaaag cctttcccgg cgcgatgatc cctgaccgaa tacgcggcat aattaaagct
     2281 caaatggccg agggtctcgt aaaaggagag gacccttgat atgccatgcg caaggtctgt
     2341 tacatccacc tcggaaagga gtccgtaatc aggtactgaa tcgtgaactg ccatgacttc
     2401 gttattgccc atgggagaaa agggtacaag ccagttccac cggccttgcc gggcaacata
     2461 ccgggaacct tccctttctt ggtcgattag gtccgtgaaa taggaagaac cgtgctcgcg
     2521 aaagtaggaa tttcctgcct cgatgagtct cctgtgatat gaatagggga ccggcgtgat
     2581 gagcatctgg agatggggat ggaccagact ggcccctgca ggaaagagat aatttgcggt
     2641 gactgcggta aaggaagcag aggggtcctt ctgatacacg atcttcagaa actcctgagc
     2701 agcagtaagg ccatcagcca aaaggtcggg acggaattca gaaagcttca gaaaatgggc
     2761 cttgcagaga cagaccacgg gatgatacgt tccgagggaa aagagattcg gaaagagaac
     2821 cgcctcgccc acacgcagac gtccttcagc aacaaaatcc gaggtgaacc ttggcgtacc
     2881 cttctcgacc ccttccccac agaagaagca attcttaccg ctctcttcca ccagccgcct
     2941 gataagatcc tggtcacatt caacaaaaaa tgcctttgcc ttgtccttaa gataggggtt
     3001 gtgaatgctt acatctccca ggagcgggtc tttgcggatt tcgagcatgt ggaggtcttc
     3061 agcaaagtcc ctgagaggat tgtgaaacct cactccagat tgaatgcgtt caaatactat
     3121 cctcgccatg gggcctctca tgtatctgga gaaaaggact ttaagttgtt ctcacccagg
     3181 agatcgagcc aggaacaatc ccgtccatca cccgac
//
LOCUS       DATKOU010000206        12768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1075089, whole genome shotgun sequence.
ACCESSION   DATKOU010000206 DATKOU010000000
VERSION     DATKOU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075089"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..949)
                     /locus_tag="VMU21_09565"
     CDS             complement(26..949)
                     /locus_tag="VMU21_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUO77809.1"
                     /translation="MKHKGFIDLHTHGLKSYDTKTADPRDILKTAELHGRTGVVAILP
                     TIYAAPIDRMRENMEAVRKAMEIQRGKGRSRGSASERPITCHNGISVILGLHLEGPFL
                     NPLRAGALDRDFFIQPTMASLEKLIDGYGEIIKIITLAPEMPGALTVIKKCSDKGIKI
                     HMGHSDATYKEAERGKRAGATGVTHIFNAMRPFHHREPGLTGFGLMDEGIYVEVIADG
                     VHLHSDVLKFLFRIKRHDRIILVSDSVKGAFKGRRPLLKERGVLAGSGMTLSESVGVL
                     EKIGIHSDAIIKAGMLNPERYLRTASLMAGQ"
     gene            complement(982..1176)
                     /locus_tag="VMU21_09570"
     CDS             complement(982..1176)
                     /locus_tag="VMU21_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HUO77810.1"
                     /translation="MNVIINIDTCIGCGTCQEICPAVFHLNETIGKAEVIDPDACEYV
                     GCCEAAEENCPVAAITLEED"
     gene            complement(1173..2117)
                     /locus_tag="VMU21_09575"
     CDS             complement(1173..2117)
                     /locus_tag="VMU21_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77811.1"
                     /translation="MKKYKEISLKNIKTYPLSSRKNKVEIELSALPHARGALFRTFME
                     GLPSFLAASDLKAVVRSIVKARRNQRPVVVGMGAHPIKVGVAPLLIDLMKRGIVTAIA
                     ANGACIIHDFELSLTGRTSEDVAREVCTGAFGMARETGRELNSAINRGVKKGYGIGRS
                     VGEYIFLKRKGLSDRSIFAEGYRLDIPVSVHVAMGTDIIHMHPHADGAMIGEGSLRDF
                     RLLASVVADLQGGVYINLGSAVIMPEVFLKALTIARNLGHRVDDITTVNMDFIQHYRP
                     RENVLIRPTLRKGRAYALTGHHEIMFPLLAAALIEEMG"
     gene            complement(2162..2857)
                     /locus_tag="VMU21_09580"
     CDS             complement(2162..2857)
                     /locus_tag="VMU21_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO77812.1"
                     /translation="MFIKLSVVIPCYNEVHTIEKVVKVVRMSPYEDKELIIVDDCSTD
                     GTREKIKSKVEPHVDKVVYHDVNQGKGAALRTGIKEATGDIIIIQDADLEYDPNEYPR
                     LVKPIVEGKADVVYGSRFMGHEPHRVLYFWHRLGNMALTLLSNMFTNLNLTDMETCYK
                     VFKRDIIQSIEIEESRFGFEPEITAKIAKKQCKVFEVGISYYGRTYAEGKKVGWKDGL
                     RAIFCIIKYNLFR"
     gene            3005..3301
                     /gene="rpsT"
                     /locus_tag="VMU21_09585"
     CDS             3005..3301
                     /gene="rpsT"
                     /locus_tag="VMU21_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HUO77813.1"
                     /translation="MPAKPAPKKNLSALKRARQAEKNKLRNKAVKTEIRTVSKKVETA
                     VDKKDKEEAHKAFMEASEVITKAASKGIIHKNTASRKISRLARVANTVLRPEAV"
     gene            complement(3282..4166)
                     /gene="holA"
                     /locus_tag="VMU21_09590"
     CDS             complement(3282..4166)
                     /gene="holA"
                     /locus_tag="VMU21_09590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HUO77814.1"
                     /translation="MSIKHFIVEIGKGLTSPAYFLYAEDAYLLKEAGLMAGELVPQTE
                     RDFSFNVFDLDGIDEIPPFEQILDVANTVPFMGKRRVVVLENVQELAKKEMPLLESYL
                     ENPSPYSLLILLHRGNPKTQWKGLIRMVKAIPLDIRPQEIPLWIREKARKKGLDITHD
                     AIEYLVGVLGPDVGLLSSELEKLALIGKSHIDTQDIISIVRGSGDYDVFDLVNALRNK
                     DAGKVFRVARGLQATQESYGLLGAINWHYSRMATREHGRKAYYARVFQLLNEADIRIK
                     TSGGTFPLEYLLIRLLQA"
     gene            complement(4253..4810)
                     /gene="lptE"
                     /locus_tag="VMU21_09595"
     CDS             complement(4253..4810)
                     /gene="lptE"
                     /locus_tag="VMU21_09595"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HUO77815.1"
                     /translation="MKIKYHCSMVDAEERTIWMAGETMRNGLLILVLVFGFFLCGCGY
                     IIQGKANLPFQTIAINKIVNKTFEPKLEDRMQVALVNELMVEGFVIDSNSAYRINGAI
                     TQFELRTLSEKSGVATEYEVIIKGDFKLVDPSGKAKELRNHGVFIVSFSSSEQLQTVM
                     ALKELATERALRDLSSEIVASIIYQ"
     gene            complement(4807..7362)
                     /gene="leuS"
                     /locus_tag="VMU21_09600"
     CDS             complement(4807..7362)
                     /gene="leuS"
                     /locus_tag="VMU21_09600"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HUO77816.1"
                     /translation="MEERYIPQKVELKWQHYWSENKLFNTDAGKEKEKFYCLEMFPYP
                     SGKIHMGHVRNYAIGDVIARYKRMRGCNVLHPMGWDAFGLPAENAAIKEGVHPAQWTY
                     ENIKYMKTQLNRMGLSYDWDREVTTCSPEYYRWNQWFFLKMFKRGLAYKKSSSVNWCQ
                     SCATVLANEQVIDGKCWRCDSEVIQKELEQWFFRITQYADELLEGCDRLKGWPENVVA
                     MQRNWIGRSEGVEMGFPLEGMDEKIRIFTTRPDTLWGATFICLAASHPLSEKLGMDKE
                     KLKGMKTKYETMDEKEGFFTGRYAINPMNLERIPVYIASFVLMEYGTGAIMSVPAHDQ
                     RDYEFARKYNLPIRIVIQPEQQPDGERLDLGVMREAYEHDGILVNSGQFTGLKSDSAR
                     REIGKYIEERGLGKRVINYKLRDWGISRQRYWGTPIPIIYCKECGIVPVNEDDLPVVL
                     PEDVTFTGKGGSPLLDLPGFLRVTCPRCGVEGRRETDTMDTFVDSSWYFVRYCSGRDE
                     EALIKDHISYWMPVDQYIGGVEHAVLHLLYSRFFTRVLRDMGILTFDEPFDNLLTQGM
                     VCMETLKCPEHGWIFPSEVKDEKCLHCGSPVLRGRVEKMSKSRKNVVDPDHLINRYGA
                     DTSRLFSLFAAPPEKDLEWSEKGVEGAHRFLNKVWGIVYKNKSNMIHSGSEVNQLPVL
                     QASAQSLVRKTHQTIRRVTNDIEREYHFNTAIAGLMELVNELSAVKPQNNGEWSVYRH
                     SVETLLLLLSPFSPHIAEELWEAVGNTPGISLQRWPQWSEDLAKDEEIELVIQINGKV
                     RAKMMIPPGLSDEAIQQRTLGEPRIREMIEDKAVKKIFVVKGKLVNIVTGIDR"
     gene            complement(7686..8345)
                     /locus_tag="VMU21_09605"
     CDS             complement(7686..8345)
                     /locus_tag="VMU21_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUO77817.1"
                     /translation="MPRPLPPQRVKPQLPQRESPAVALPPSAQPQEKAVERGIAPDNR
                     PGSNTQDQALPGQGSPPGSNALTGSGMPGSPRSGGTAPPSSSLRERLFDREVMDKLAR
                     REEKRHDSSITFDTNEFKYESYMMKLKSRIEGIWRYPQDAARRGLHGDLYIKFTIKKN
                     GRLEDVELVRTSGNRSLDEAAMQALKEGEPYWPLPDEWGKDVLTITGHFVYSMYGTYI
                     R"
     gene            complement(8629..9750)
                     /locus_tag="VMU21_09610"
     CDS             complement(8629..9750)
                     /locus_tag="VMU21_09610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUO77818.1"
                     /translation="MLRTEVLVIGGGPAGSTAARFLARNGVDTLLVERNFSFVKPCGG
                     GIPSFLLQEMDLPELSVKRYVDTIKVVSPGGNTLDIPLKGGTIAIIERGEFDNVLRSE
                     AEKSGTRLLQGEFRRFTDLSRTVTAEVATSPEISFSGSSFPRTINVEADYVIAADGVN
                     SRVRTALGIKPSPSFLTLSEKLRNKNTDVCEFWFGSSHAPLCYSWVFPHREGVSVGTG
                     TLEHARITHLWRKFVERTGLHTAGPQRGYKIPLWQGDLYHSGRVLFVGDAAGQVMPST
                     YEGIYYAMKAGELAGSAIANGKIGEYKKLWEKGFGRRFSLMKRLWTYFMASDQRLEKM
                     VQLHKKPEVQEASMNLWLRKDPGKRSLLTYIKIFSKFLS"
     gene            complement(9839..10354)
                     /locus_tag="VMU21_09615"
     CDS             complement(9839..10354)
                     /locus_tag="VMU21_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HUO77819.1"
                     /translation="MVMRVYLSENAFIDVLLSSAEVYKRECLGYLLGYRLEDRFIVEH
                     AFSFQTANRRHKGVVVHEKNHKKVEPILAKFDKLQIIGDFHSHTQFGPTKGLPFPSEE
                     DIKGMKQGQIYIIIAINNNEKTVGWGENRDGTISGSVGNFFFKISAYFLNKKSSKRAR
                     IHCPFPPKFLN"
     gene            complement(10390..10863)
                     /locus_tag="VMU21_09620"
     CDS             complement(10390..10863)
                     /locus_tag="VMU21_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77820.1"
                     /translation="MEIRKILFPTDISEGSAHAVPFVVDLTKHYGAGLYIIHVVYDIA
                     RVTGWHIPHVSMDEMYRDIETNAMKEIEKCCIEELRGYKNIEYKVIKGVPHEEILKFA
                     EENHIDMIIMGTHSRKGLDRVFFGSTAERVVRNAKCPVLTVGPPVHRKTAEAEKK"
     gene            complement(10865..11857)
                     /gene="galT"
                     /locus_tag="VMU21_09625"
     CDS             complement(10865..11857)
                     /gene="galT"
                     /locus_tag="VMU21_09625"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HUO77821.1"
                     /translation="MHELRKDPLLGRWVAVMSDSKSPEEYTIHPENSEETSCVLCPGR
                     EKETPPEIAATREGNKWWTRVIPNFKPVLHVEGDLDRKGMGMYDKMNSIGANEIIIDS
                     PEHNKSPEEMGSEQMVRVIRVYGDRVTDLEKDQRLRYIHIFKNSGRGAGAAYAHPHSE
                     IMATPIIPRLVKEELDGAKQYYAYKERCIFCDIMREELRVGDRIIFETREFLAFSPFA
                     PQFPFEFWVLPKKHACAFQDIGEDEKEDLATMLLTVLRKMRRVLKNSPYNYVIHTAPN
                     RIPRRNHWHTLGDDFHWHMEIIPRLLRTSGVEWGSDFYVLTTSPEDAAKYLREA"
     gene            complement(11857..12735)
                     /locus_tag="VMU21_09630"
     CDS             complement(11857..12735)
                     /locus_tag="VMU21_09630"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HUO77822.1"
                     /translation="MERYWTFFFKKNNYFNVLVKQLSYLHNQYFCYNSGMLLAIDIGN
                     SSINIGFFDRTDLPRTLKIPTHPKRPGGVYKNKIHDFLSKNHMETAIRGAIISSVVPD
                     LTEKVCNAARDLGAKHPFVVSASLPAGLTYGVEKPEEIGSDRIANIVAASELFGSPVL
                     VVDFGTATTITALKNREFLGGAILPGIELMSNCLKKGTAKLPKVDLVPGDGQVPPHAM
                     GKNTTMCILSGIIYGTAGAVERLIYEMELGESCRFKVVVTGGNSFIMRGVLSRDFSLD
                     PDLTLKGLRLIHERNI"
BASE COUNT         3084 a         3442 c         2708 g         3534 t
ORIGIN      
        1 cgtgctgaaa ataaagaatt ttttctcatt gtccggccat gagtgacgca gtgcgcagat
       61 atctttcggg attgagcatt cccgccttaa ttattgcgtc actgtgtatg ccaatctttt
      121 ccaaaactcc taccgactcc gaaagagtca tgccactccc tgccagaaca cccctttcct
      181 taagcaaagg tcttctcccc ttgaaagcac ctttcaccga gtctgacaca agaattatcc
      241 tgtcgtgacg cttgatgcgg aagagaaatt tgagcacatc ggagtgaaga tggactccgt
      301 cagcaatcac ctccacatag attccctcat ccataagccc aaatcctgta agccctggtt
      361 ccctatggtg aaaaggccgc atggcattaa aaatatgggt gactcctgtg gcacccgccc
      421 ttttcccacg ttctgcttct ttataggttg catcagaatg tcccatatga atcttaattc
      481 ccttgtcgga acatttctta atcaccgtga gagcccccgg catttcagga gcaagggtga
      541 ttatctttat tatctctcca tacccgtcaa ttagtttctc aagggaagcc atagtgggtt
      601 gaatgaaaaa gtctctatcc agcgctccgg ccctcaatgg attcagaaag ggtccttcca
      661 gatggagacc taaaatgact gatattccgt tatggcaggt aataggtctt tcggatgcag
      721 aacctcggct gcgtcccttc ccccgttgta tttccatcgc ctttctgact gcttccatat
      781 tttccctcat cctgtctatg ggagcagcat atatagtggg gaggatggca acaactccgg
      841 ttcttccatg tagctcggct gtttttaata tatcgcgggg gtcggcagtc tttgtatcat
      901 agcttttcag tccgtgcgta tggaggtcaa taaatccctt atgcttcatg cgtcattaca
      961 catgtgtgac acgagaaaac actaatcttc ctcgagtgta atagcagcca caggacaatt
     1021 ctcttctgca gcctcgcagc aacctacata ctcacacgca tcagggtcta tgacctctgc
     1081 cttaccgatc gtctcattga ggtgaaatac cgcaggacaa atctcctggc atgtcccgca
     1141 gcctatacac gtatctatgt ttattattac gttcatccca tttcctcgat aagggctgca
     1201 gcgagtagag ggaacattat ctcatgatgc ccggtaagcg cgtatgccct tcccttcctg
     1261 agagtgggtc tgatgagtac attctccctt ggtctgtagt gttgaatgaa gtccatattc
     1321 actgtggtga tgtcatcaac ccggtggccg agattccttg caatggttaa tgccttaaga
     1381 aagacctccg gcatgataac cgcagagcca agattaatgt atacgcctcc ctgcaggtcg
     1441 gccaccaccg aagcgagcag cctgaagtcc ctaaggctcc cctcgccaat cattgccccg
     1501 tctgcgtgcg ggtgcatatg gataatgtcc gtgcccatgg caacatgaac gctgaccggg
     1561 atgtcaagtc tgtatccctc agcaaagatg ctcctgtcac tcaaaccttt tctctttaga
     1621 aagatatatt ccccaaccga tcttccaatg ccgtaccctt tcttcacccc cctgttgatt
     1681 gcactgttta attcccttcc cgtctccctc gccatgccaa acgctccggt acagacttcc
     1741 cgcgcaacat cctctgatgt cctcccagtg agagaaagtt cgaagtcatg aatgatacat
     1801 gcgccattcg ctgcaattgc ggtgacgatc cctctcttca tcaaatcaat gagcaacggg
     1861 gcgaccccga ctttgatagg atgggctccc atccctacta ccacaggcct ctggtttctc
     1921 ctggccttaa caatggaacg caccactgcc tttaggtcag atgcggcaag gaaggagggg
     1981 agaccctcca taaacgttct gaacaacgca ccccgtgcat ggggtaatgc agagagttcg
     2041 atttccacct tgttttttct tgaagacaag ggataggttt ttatgttctt gagggagatc
     2101 tctttatact ttttcatacc ttccattccg cccagggtat cgaattcccg aaaagcatcc
     2161 attaacggaa taaattatat ttgataatac aaaaaattgc acggagacca tctttccatc
     2221 ctactttctt tccttccgcg tatgtcctcc cgtagtagga aatacccacc tcaaacactt
     2281 tacactgctt cttggcgatt tttgcggtaa tttcgggttc aaagccaaat ctgctttcct
     2341 ctatctcgat tgactggata atatcccttt taaatacctt gtagcaggtt tccatatcgg
     2401 tcagattgag atttgtgaac atattcgaca acagagtcaa tgccatattt cctagtctgt
     2461 gccaaaaata cagtacccta tgcggttcat gccccataaa ccgggagccg tacaccacat
     2521 ccgcctttcc ttccactatt ggtttcacaa gtctgggata ctcattggga tcgtattcca
     2581 aatcagcatc ctggattata ataatatctc ctgttgcctc tttgatgccc gttctcagtg
     2641 ctgcgccctt tccttgattc acgtcatgat acaccacctt gtccacgtga ggttcaactt
     2701 tactctttat tttctccctt gtcccgtcag tggaacaatc gtcaacaatg ataagctctt
     2761 tgtcttcgta gggggacatc cgcaccactt tcactacctt ttcaatggtg tgtacttcgt
     2821 tatagcacgg tatgaccaca gagagtttaa tgaacacctc ttgacctttt tctgcattat
     2881 gttatggctt attataccct aagccgcaac ccattaccgt tcctttatca ccattgattt
     2941 ttggcagatg ttgtgttaat attatctttt ttaataaacg aaaattcgtc tcaaggaggg
     3001 aacattgccg gcaaaaccag caccaaaaaa gaacctgtca gcacttaaaa gggctcgtca
     3061 ggcagaaaag aacaagttgc gtaataaagc ggtaaagacc gagatcagga ccgtgagcaa
     3121 gaaagttgaa accgctgttg acaagaagga taaggaagaa gcccataagg cctttatgga
     3181 agcctcagag gtcattacta aggctgcttc aaagggcatc atccacaaga atacggcgtc
     3241 gagaaagata tcccgccttg caagggttgc aaatactgtt cttaggcctg aagcagtctg
     3301 atgaggagat actcgagggg gaatgtccct cccgaggttt ttatccggat gtcagcctca
     3361 ttgagcagtt ggaacaccct ggcataataa gcctttcttc cgtgttccct cgtagccatc
     3421 cggctatagt gccaattaat ggcacccagt agcccgtagg actcctgagt cgcctgaagg
     3481 cctctcgcaa cccgaaatac cttaccggca tccttattcc tcagggcatt cacgaggtca
     3541 aagacatcat aatcgccgct tcctctgaca atacttatta tgtcctgagt atctatatga
     3601 ctcttgccga taagggcaag tttttccaac tcggaagaca ggagacccac atcagggcct
     3661 aggacaccca ccagatactc tattgcatca tgcgtgatat ccagcccttt cttgcgtgcc
     3721 ttttccctta tccacagggg aatctcctgc ggccttatat ccagtgggat ggctttgacc
     3781 attcttatca accctttcca ttgggtcttc ggattccccc tgtgcaaaag tataaggagc
     3841 gagtagggag aaggattttc aaggtaactc tcaagtagcg gcatttcttt ttttgcaagc
     3901 tcctgaacat tctcaagaac aacaaccctc ctttttccca taaaggggac cgtatttgct
     3961 acatcaagga tctgctcaaa gggcggtatc tcatcaattc cgtccaaatc aaaaacgtta
     4021 aaagaaaaat ccctttctgt ctgagggacc aactccccag ccataagacc agcctccttg
     4081 agcagatacg catcttcagc gtataagaaa tacgccggtg atgttagtcc cttgccgatt
     4141 tcgacgataa aatgtttgat gctcatttgc cttcagtatt gatgcaaagg ctaaggaatg
     4201 attatccagg ccctacacgc tcaaaagttc ccgggttttc cctgcgaatc gttcattgat
     4261 atatgatgga cgcgactatt tcgctcgaaa ggtctctcag tgccctttct gtggccagtt
     4321 cctttaatgc cataacagtc tggagttgct cagaactgga aaaagagaca atgaacactc
     4381 catggtttcg caattccttg gccttcccgg atggatccac aagcttaaaa tctcccttta
     4441 tgatgacctc atactcggta gccacacccg acttctcgga aagagttctc aactcgaact
     4501 gagttatggc cccgtttatt ctgtaagcgg aattactgtc tatcacaaag ccctctacca
     4561 ttagttcatt cacaagcgca acctgcatcc tgtcttccag cttcggctca aaggtcttgt
     4621 tcacaatctt attgatcgca atagtctgaa acggcagatt tgcctttccc tggatgatat
     4681 aaccacatcc gcacaagaaa aatccaaaaa ctaagactaa aataagaaga ccgttgcgca
     4741 tggtttcccc cgccatccaa atcgtgcgtt cctctgcgtc aaccattgaa cagtgatact
     4801 tgatcttcat ctgtcaatcc ccgtgacaat atttacaagc ttgcctttca ccacaaaaat
     4861 cttcttgaca gctttatcct caatcatctc ccttatcctc ggttcgccca gcgttctttg
     4921 ctgaatagcc tcatcagaaa gacccggggg gatcatcatc tttgccctga cctttccatt
     4981 tatctggatc acaagctcga tctcctcatc ctttgccaga tcctcactcc attggggcca
     5041 tctctggagc gatatgccag gggtgttgcc taccgcctcc cacaactctt cggcaatatg
     5101 cggggagaag ggagagagca gcaaaaggag cgtctcgacc gaatgtctgt acaccgacca
     5161 ttctccattg ttttggggct taactgctga gagttcattc accagttcca tgagcccagc
     5221 aatggcggta ttgaagtggt actccctctc aatgtcattg gttacccgcc tgatcgtctg
     5281 gtgcgtcttc cgcacaaggc tctgggcaga agcctgaagg accgggagtt gattcacttc
     5341 ggatccggaa tgaatcatgt tggacttatt tttatacacg atcccccaca ccttgttcag
     5401 aaaacggtgt gccccctcca ctcccttttc ggaccactcc agatcctttt caggaggggc
     5461 tgcaaaaaga gaaaaaagcc gcgaagtgtc cgcgccatac ctgttaatga gatggtcggg
     5521 atcaaccacg tttttccttg acttggacat cttctcaacc cttcccctca acaccggact
     5581 gccgcaatgg aggcattttt catccttgac ctcagacgga aaaatccatc cgtgttccgg
     5641 acatttcagg gtttccatac ataccattcc ctgggtgaga agattgtcga atggctcatc
     5701 aaacgtcagt atccccatgt ccctcaggac acgggtaaag aatctcgaat aaaggaggtg
     5761 cagaaccgca tgttcaaccc caccgatata ctgatccaca ggcatccaat aggagatgtg
     5821 gtcctttatc agggcttctt catcccgccc tgaacaatac cggacaaaat accaggatga
     5881 gtcaacgaag gtgtccatgg tgtctgtttc acgtctccct tcaacgccgc atcgaggaca
     5941 tgtcaccctc agaaaaccgg gcagatctag aagcggtgac ccgcctttgc cggtgaacgt
     6001 tacatcttca gggagcacta ccggaagatc atcttcattc accggaacga tgccgcattc
     6061 tttacagtag ataatgggaa tgggggtccc ccaatatctc tgccttgaga tcccccagtc
     6121 gcgaagcttg taattaatca ccctcttacc gagccctctt tcctcaatat acttcccgat
     6181 ctctcttcgc gcactatcgc tcttcaatcc ggtaaattgc cccgaattca ccagtatccc
     6241 gtcatgctcg tatgcctcgc gcattactcc caagtccagc ctttcaccat caggctgctg
     6301 ttcaggctgt attacgattc ttatgggcag gttatatttc cttgcgaatt cgtaatctct
     6361 ctggtcatgg gcagggacgg acataatggc gccggtcccg tactccataa ggacaaaact
     6421 ggcaatgtag acgggtatcc tctcaagatt catcgggttg atggcataac gtccggtaaa
     6481 gaatccctct ttctcatcca tagtttcata ctttgtcttc attcccttga gcttttcctt
     6541 atccataccc agtttttctg aaaggggatg gcttgctgca agacagataa aggtggcacc
     6601 ccagagcgtg tcgggccgag tggtgaatat ccggatcttc tcatccatcc cctctagggg
     6661 gaaccccatc tcgacaccct cacttcgtcc aatccagttt ctctgcatcg ccaccacatt
     6721 ctccggccag cccttgagcc tgtcacatcc ttcaagcagt tcgtcagcgt actgagttat
     6781 cctgaagaac cattgttcca gctccttctg aatcacctca ctgtcacatc tccagcattt
     6841 cccgtcaatc acctgttcat tggcaaggac ggttgcgcat gactggcacc agttgacaga
     6901 ggaggacttt ttataggcaa gccccctctt gaacatctta agaaaaaacc attgattcca
     6961 ccggtaatac tcaggactgc aggtggtcac ctctctgtcc cagtcatagc tgagccccat
     7021 acggtttaac tgtgtcttca tatatttgat gttctcgtac gtccattgcg caggatgcac
     7081 cccctctttt atagctgcat tctctgcagg gaggccaaag gcatcccatc ccatgggatg
     7141 gagcacgttg caaccacgca tgcgtttata tctcgcgatc acatctccaa tggcataatt
     7201 gcgcacatgt cccatgtgta tcttccctga tgggtaaggg aacatttcga gacagtagaa
     7261 tttctccttt tcttttcccg cgtcggtgtt gaatagctta ttctctgacc agtagtgctg
     7321 ccatttcagc tcaaccttct gcgggatgta tctctcttcc aagactttca cctccaaaaa
     7381 cagtaaaaat taccataaac acaggtgcgt tagcaagaat caggacctgt tggcagtgcc
     7441 taaccctgat aattcataaa ctcgccagag gtgtccggct ccaatggcaa tccgcctgag
     7501 gcggaagggt tatgtgcgtg tccgcataag cggtccttgg cgatacatat ggacagagtg
     7561 tcgttacgta tagagagccc gtacagtcgt gaagggccat tgtcctgcca tgagccggaa
     7621 acctctggca ttcgatgaga aacgaataaa ttataccggt ctattgatgt tagaatcgca
     7681 atgtctcatc gtatgtacgt cccgtacata gaatagacga agtggccggt aatggtgagg
     7741 acatctttgc cccactcatc aggcaatggc cagtacggtt caccctcctt gagtgcctgc
     7801 atcgccgctt catcgaggct cctgttccca gaagtcctca ccagctccac atcctctagc
     7861 ctgccgtttt tcttgatggt gaactttata taaaggtcgc cgtgtaatcc cctcctggct
     7921 gcatcctgag gatatctcca tatcccttcg atccttgatt tcagtttcat catatagctt
     7981 tcatacttaa attcgttcgt gtcaaaggtg atgctgctgt cgtgcctctt ttcctctctc
     8041 cttgccagtt tgtccatgac ctctcggtca aagagccttt cgcgaagaga ggatgaaggc
     8101 ggggctgttc ccccactcct tggtgaacca ggcattccac tccccgtcag tgcattgctg
     8161 ccaggggggc ttccctgccc tggcaaggcc tggtcctggg tgttgctccc ggggcggttg
     8221 tccggggcga ttcctctctc aacggccttc tcctgtggtt gagcagaagg gggaagggca
     8281 actgcaggac tttccctctg cggtagttgg ggtttcaccc tttggggcgg taagggtctc
     8341 ggcacgacag ggcctcttcg tggtgtcagg ctcccttgtt tcgctgcccc ggtcgatgga
     8401 gcggcaggca tttccctcct cacttcctcc ggagttatga gtcttgtgat aaagggcttt
     8461 ggtgattctt ttttgctcgg tgggtaaaga aagatcatca caagcaccag gagatgtact
     8521 atcagggaaa atgtcagagg taacttccac tggggcgtca tagaatcaat tccatgtaag
     8581 agatagtatt tcatcgcttt gggttatgtc ttttcttgtt ggttgacgct aactcaagaa
     8641 tttgctgaag atcttgatgt aggtaagaag gcttctcttt cccggatctt ttctgagcca
     8701 gaggttcatg gatgcttcct gaacctctgg tttcttatgc aactgcacca ttttttcaag
     8761 acgttgatca cttgccataa aatatgtcca gagtcttttc attagtgaaa accgtctacc
     8821 gaagcctttt tcccagagct tcttatactc cccaatcttc ccgtttgcga tcgcacttcc
     8881 tgccagctcc cccgccttca tggcatagta tatcccttca taggttgatg gcatcacctg
     8941 gcccgccgca tcacccacaa aaaggactct ccccgaatgg tacaaatctc cctgccacag
     9001 aggtatctta tacccccttt gagggccggc ggtatgcagc cctgtcctct ccacaaattt
     9061 cctccacaag tgtgtgatcc tggcatgttc caaggtacct gtgccgacag aaactccctc
     9121 cctgtgggga aagacccacg aatagcagag cggtgcatgg gatgagccaa accagaattc
     9181 acagacatca gtatttttgt tccgtagctt ttcggacagg gtgagaaatg agggagaagg
     9241 tttgatcccc aatgccgtcc tcacccttga gtttaccccg tctgccgcaa ttacatagtc
     9301 ggcttccaca ttaatggtgc gcgggaatga ggacccggaa aaagagattt caggagaagt
     9361 ggcgacttct gcggtcaccg tcctgctcag atcagtaaaa cgcctgaatt ctccctggag
     9421 aagccttgtc cccgacttct ccgcctcact cctgaggaca ttatcaaact cgccccgttc
     9481 gataatggcg atagtccctc ctttcagcgg gatatcgagg gtattccccc cgggagagac
     9541 aacctttatg gtatccacat atctcttaac agaaagttca ggaagatcca tttcctgcaa
     9601 caggaaagaa gggatgcccc caccgcatgg cttgacaaag gagaagtttc tctccacaag
     9661 aagggtgtcc accccgttcc ttgccaggaa acgtgctgcg gtagaaccag cggggccacc
     9721 ccctattacc aacacctcgg tcctgagcat tactttacaa aagtaaaaga ggggctttca
     9781 gaatcatata cggggtccct cttttcccag agtaggtttc tcaataggta ggacctcact
     9841 aattcagaaa tttcggaggg aaagggcagt gtatccttgc ccgcttcgag gatttcttat
     9901 tgagaaaata ggctgatatc ttaaaaaaga agttccctac agaacccgag atcgttccat
     9961 ccctgttctc gccccatcct acggtctttt cattattatt gatggcaata ataatataga
    10021 tctggccctg tttcattcct ttaatatcct cttcgctcgg aaaggggagc ccttttgtgg
    10081 gaccgaattg cgtgtgggaa tgaaagtccc ctataatctg taacttgtca aatttagcga
    10141 gaattggttc cactttcttg tgattctttt cgtggactac aacgccttta tgccttctgt
    10201 tcgccgtttg aaagctgaat gcgtgctcga cgataaatct gtcctccagc cggtagccga
    10261 gcaaatatcc aaggcactcc ctcttgtaca cctcagcaga actgagcagt acgtcaatga
    10321 aggcattctc tgaaagatag accctcatca ccataccgga gagatcttgc gaaactcctc
    10381 catttccttc tacttcttct ctgcttcagc agttttccgg tgaacgggag gtcctacggt
    10441 aaggacaggg cactttgcat tcctcactac cctctccgcg gtactgccaa aaaagaccct
    10501 gtcgagtccc tttctgctgt gggttcccat aataatcata tcaatatgat tctcttctgc
    10561 gaatttcagt atttcttcat ggggtactcc cttaatgacc ttgtattcga tattcttgta
    10621 tcccctcagt tcctcgatac agcatttctc tatctccttc atggcatttg tctctatgtc
    10681 cctatacatt tcatccatac tcacatgggg gatatgccac cctgtcaccc tcgcgatatc
    10741 gtagacaaca tggatgatat aaagccctgc tccataatgc ttcgtgaggt ccacgacaaa
    10801 gggaaccgcg tgtgcagaac cctcggaaat gtcggtgggg aaaagaatct tcctgatctc
    10861 catatcaagc ctcccttaaa tattttgctg catcctccgg ggatgttgtc aagacgtaaa
    10921 aatccgaacc ccactcaacg ccgcttgtcc tgagaaggcg gggtatgatc tccatgtgcc
    10981 agtgaaaatc atcgccgagg gtatgccaat ggttacgcct ggggatcctg tttggtgcgg
    11041 tatgaattac ataattgtaa ggggaattct tcagcacacg cctcattttc cgtaacactg
    11101 tcaaaagcat ggtggcgaga tcctcctttt cgtcctctcc tatgtcctga aaggcacacg
    11161 catgtttttt gggcaatacc cagaactcaa aggggaattg gggggcaaag gggctgaaag
    11221 caaggaactc tctggtctcg aatatgatcc tgtcgcccac cctgagttcc tcccgcatga
    11281 tatcacagaa tatacagcgt tccttatacg cataatactg cttcgctcca tccaattctt
    11341 ccttaacaag cctaggaata ataggtgtcg ccataatttc ggagtggggg tgagcatagg
    11401 ctgcccctgc accccttccc gaattcttga atatgtggat atatcgtaac ctttgatcct
    11461 tttcgaggtc cgtcaccctg tcaccataga ctcttataac cctgaccatc tgctcacttc
    11521 ccatctcctc aggggatttg ttatgttcag ggctgtctat aataatttcg ttcgctccaa
    11581 tgctgttcat tttgtcatac attcccatgc cttttctgtc aagatcacct tctacatgaa
    11641 gcaccggctt aaaattggga atcacccttg tccaccattt atttccctcc ctcgtggctg
    11701 caatctctgg cggggtctcc ttctccctgc ctgggcacaa gacacatgag gtttcctcag
    11761 agttttctgg atggatcgtg tattcctcgg gagattttga gtcactcatt accgcaaccc
    11821 atctgcccaa caaagggtcc ttcctcaatt catgcatcag atattcctct catggataag
    11881 tcgcaggcct ttaagggtaa gatcaggatc cagagagaag tccctggaga gaacaccgcg
    11941 cattataaag gaattcccgc ctgttacgac aactttgaac ctgcacgatt cccccagctc
    12001 catctcgtat atgagccttt ctacagcccc cgccgtacca tatataatac cagaaagtat
    12061 gcacatagta gtgttttttc ccatggcgtg agggggaacc tgcccatctc ccggaaccaa
    12121 atccaccttg gggagttttg ccgttccttt tttgagacaa ttgctcatta attctattcc
    12181 cggcaatatt gccccaccaa gaaattccct gttcttaaga gcagttatgg tggttgcagt
    12241 gccgaaatcc accacaagca ccggtgagcc gaatagttcg ctggctgcaa ctatattggc
    12301 aatcctgtca cttccaatct cttcaggctt ttccacgcca taagttagtc ctgcaggtaa
    12361 cgaagcgctc accacaaatg gatgtttcgc tccaagatcc cttgccgcat tgcaaacctt
    12421 ctctgtaagg tcgggaacca cagaggatat tatagcgccc cttatggctg tttccatatg
    12481 gttttttgaa aggaaatcat ggattttatt cttgtataca ccacctggtc ttttgggatg
    12541 ggtagggatc ttcagcgttc taggaaggtc tgtccgatca aaaaaaccaa tatttattga
    12601 tgagttacct atatctatgg ccaaaagcat accactatta tagcaaaaat actggttatg
    12661 taagtatgaa agttgtttta ctaacacatt gaaataattg ttttttttaa agaaaaaggt
    12721 ccaatacctt tccaagggac cggcatttat tttaatttgc cgttagga
//
LOCUS       DATKOU010000207         9632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1457100, whole genome shotgun sequence.
ACCESSION   DATKOU010000207 DATKOU010000000
VERSION     DATKOU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457100"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VMU21_09635"
     CDS             complement(<1..467)
                     /locus_tag="VMU21_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO77823.1"
                     /translation="MMIFKYNGLFGRALLALSVVVLIGIPVISSGADDLQKIRVELNN
                     SDWNMRLAAVEKLSNRKDEDTIDLLIYITEMRTEYWPVKIKAITLLGDSGNPRAIEPL
                     LATFNDSFMNWECPSIKSYAAAALGNYKQDPRVLETLIEGVDDKELLTREASI"
     gene            666..2222
                     /locus_tag="VMU21_09640"
     CDS             666..2222
                     /locus_tag="VMU21_09640"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HUO77824.1"
                     /translation="MSIITAYLITFIASFCILVIEIVAGRILAPFVGVSLYTWTSIIG
                     VVLTGISIGAYLGGKMADRFPERKTLGWLLLLSGITTLCIAPMTNLVAGYHFPTTLML
                     RILIVTTLTFFIPSCILGMISPVVVKLTLRDMKTAGNVVGRIYAVSTLGSIIGTFAAG
                     FFLISWMGTRHIILLMGLILILTALVWGSLFKSKKTVFGLLIVIPLFLIWGVYGFAFK
                     APLDAYTNYYKESDYFTLKVKEATASDGKTPLKALVLDNLTHSYVNLKDPLHIEYKYE
                     KIYAEVLKWRFSKDTPFRSLTIGGGGYTLPRYMEVSYPHASIDVVEIDPEITRVVYDY
                     LGLSRNTRIRTFNEDGRWYVMNCKERYDLIFIDAYNDLSIPYHLTTREFAQMLRDILN
                     PEGIILTNIIDNFQRGSFLPSTIRTLREVFGERNVHLISISPHFDKIHSSTFIVLTGK
                     DEVDIRDFESYLKNRHEGKAISAVVPENILNDYLKRTYSVVLRDNYAPVDNLIAPVFE
                     ERFGYNRKER"
     gene            complement(2263..3372)
                     /gene="ychF"
                     /locus_tag="VMU21_09645"
     CDS             complement(2263..3372)
                     /gene="ychF"
                     /locus_tag="VMU21_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HUO77825.1"
                     /translation="MKLAIIGLSNSGKTTVFNALTGQNIETTLYPTVSGEPHFGVVKV
                     PDRRVDTLAGIYKPKKITYATVEYIDYLGLTKGDVEQNRKVFDLIKDADAIVHVVRAF
                     ENEVVSHPLNEINPLKDIETLELELIFGDLEFVEKRLARMEESARKGKKPSEGEKRLL
                     VKCREALEKEIPLRNIHFDEDEGRAMKPLQFISIKPEVVVLNIGESDLNAEKVKNIQK
                     DAETFFGSRDKGSSGARPYASLSLCGKIEMEIAQLSAEEARVFLDDLGIQEPALNKLI
                     HVSYDLLGLISFLTVGEDEVRAWTIQKGTDAHKAAGKIHSDIERGFIRAEVVGYEDFI
                     ASGTMHAAKEKGLLRLEGKTYQVKDGDIINFRFNV"
     gene            complement(3380..3973)
                     /gene="recR"
                     /locus_tag="VMU21_09650"
     CDS             complement(3380..3973)
                     /gene="recR"
                     /locus_tag="VMU21_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HUO77826.1"
                     /translation="MTQGIIENLINELTRLPGIGRKTAQRLAFFILTMPDHEAKAIAQ
                     SITEVKDRARFCRECFNITDSEVCEICRDEGRDRKKICVVEEPSNILVIERSKFYRGL
                     YHVLLGAISPIDGVTPDKLKINELIQRVRSNNVEEVIIATNPNTKGEITAQYIREHLD
                     SHPVKVSRIAYGLPMGSDIEFADEVTLSKALEGRRQM"
     gene            complement(3970..4308)
                     /locus_tag="VMU21_09655"
     CDS             complement(3970..4308)
                     /locus_tag="VMU21_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HUO77827.1"
                     /translation="MSKKMLGDIMREAQKLQTEMQRLQAEAKTKTVEATAGGGMVTVV
                     ANGGGELISIKIERDVVNADDVEMLQDLILAAANEALRRAQQMVNEEMAKLTGGLQIP
                     GLGGLGNIFG"
     gene            complement(4301..5959)
                     /gene="dnaX"
                     /locus_tag="VMU21_09660"
     CDS             complement(4301..5959)
                     /gene="dnaX"
                     /locus_tag="VMU21_09660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HUO77828.1"
                     /translation="MSYLVLARKWRPLGFEDLIGQEPISRILSNAIVQGKIAHAYLFS
                     GPRGVGKTSTARILAKALNCEKGPTSVPCGACASCRGITDGSSIDVTEIDGASNNSVN
                     DIRDLRERVKYAPAGGRYKVYIIDEAHMLSDAAFNALLKTLEEPPSHVVFVLATTVPR
                     KIPATVLSRCQHLPFRKISSQVIKGRLKKISDTEGIQISDSALEMIARASEGSMRDSL
                     TILDQVAAFSADIKESEVKDLLGITDFRSLSELSAAVIRGDRKGIIEKVTELTERGTD
                     LRSFAKDLMHYFRDLLIAKVVQKPEEILDISESELRSINGILTTTTEDQLTLLLSEMI
                     RAESEVRGAFAPRIALEMALIRASFLTVLKPVQEAVKDIESLLAGRVVGEHAQKAHTL
                     PVPHKGKEACDERPVPVSPSEEALPADEALSAPHPAPDSEFQSVFMQKLEAVNHPLAC
                     KLLGAALRIEGDSLVITFNGGSGIHADSVKKNLETLREIAAVILQRTVSVKIEVEKKK
                     VPRKRELKDKILSEPLVKEAIELFEGRIVDVRTTDNMEDGGEHV"
     gene            complement(6046..6144)
                     /gene="ffs"
                     /locus_tag="VMU21_09665"
     ncRNA           complement(6046..6144)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VMU21_09665"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6388..6564)
                     /locus_tag="VMU21_09670"
     CDS             complement(6388..6564)
                     /locus_tag="VMU21_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77829.1"
                     /translation="MKRMMFVVLMLAFSVGCASKDYVRQQIEPLQDKINKLQTCCEQN
                     KKAMKKSFELQQKK"
     gene            complement(6665..6755)
                     /locus_tag="VMU21_09675"
     tRNA            complement(6665..6755)
                     /locus_tag="VMU21_09675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6719..6721),aa:Ser,seq:gga)
     gene            complement(7295..7669)
                     /locus_tag="VMU21_09680"
     CDS             complement(7295..7669)
                     /locus_tag="VMU21_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HUO77830.1"
                     /translation="MYGTQRCVFVNRYQKEYQKATQKLLSDWDRMTTFYSFPNEHWVH
                     LRTTNVVESPFGSVRLRTDAAKRFKKIQNATAMIWKLLQVAEKSFRMLKGYWLLQEVY
                     EGKQFVNGIRTQETKVLERMAA"
     gene            complement(7639..8325)
                     /locus_tag="VMU21_09685"
     CDS             complement(7639..8325)
                     /locus_tag="VMU21_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HUO77831.1"
                     /translation="MSGTVTIRRPRLRGAEGFESKVLPLFKRRSRELGDMLPELYLHG
                     LAKGDFDLALRGLLGSGAPLSSASIDRLKAKWQLEYEQWKQRDLSSLKVVYQWADGIY
                     VKAGLEKDKAALLIIIGALTSGKKVFLACESGYRESKESWSSVLRDLSGRGLSLGKLT
                     IADGHLGIWSALAEIHPRGKEQRCWNHKIRNVLDCFPKRIRGEAAEQLKQVPYAETLK
                     ECTELRDAFL"
     gene            8450..>9632
                     /locus_tag="VMU21_09690"
     CDS             8450..>9632
                     /locus_tag="VMU21_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUO77832.1"
                     /translation="MKGKVRTREICPQCRGKFKIVEGIDIFCERCNTRPKTFFIVLYH
                     DKAKQRISREKDGQILDSYRRAHRLLENIRKDYDDGVFSLSNYLPEEIEEFRGHKLFP
                     KWLGTKTLIAPSSLREYKRYTELYFVPYFGNQDMRQLNAGHIEDFFLSIPKAQEEHGF
                     RQLSLKSLKNISIALHTFCVWLYTRETIKRVPVFPKITPPKPPIDWITRNEQMKILAF
                     INSHHRPIFEFLYYHPVRVGEARALRRKHFKLDIMAVHVCEAFSLKETRSRKNKEPYY
                     LPIAKRFNLDVLKDKLPEAFVFTNKAGRPYTSEGLRKIWHRATKKAGLRIQLKNATRH
                     SGASQSINAGIDLATISKSLGHATLQITKDKYASMNIELLRAVVDRDNVVEFNEHVVS
                     AK"
BASE COUNT         2328 a         2606 c         2085 g         2613 t
ORIGIN      
        1 tgtatggacg cttccctggt gagcaactcc ttgtcgtcca ctccttcaat aagggtctca
       61 agcactcttg gatcttgctt atagtttccc agggctgctg ctgcgtatga ttttatggac
      121 gggcattccc agttcataaa agagtcatta aatgtcgcaa gcaggggttc tatcgccctg
      181 ggatttccgg agtctccgag caatgtgatt gccttaatct ttaccggcca gtattcggtc
      241 ctcatttccg ttatataaat cagcaggtct atggtgtctt catccttcct gttgctcagc
      301 ttttccacag cagcgagacg catgttccaa tcgctgttat tgagctccac ccttatcttc
      361 tgcaagtcat ccgcaccaga cgatattacc ggaatcccga tcagcacaac gacagacagc
      421 gccaagagtg ctctgccaaa aagtccattg tatttgaaaa tcatcatctt tcctcccttt
      481 gaaacgatag gttctttttg gttttattat acatcattat acacgggcag tgaaagcccg
      541 ttagcaatct tcgcagcagt catctgtcgt gcaaggaatt aatacactct gagagagcca
      601 aaccttgcat tcccccgagt ccagtgtgat atgatgtatc ccatatttgt ttgaagggaa
      661 tttgcatgag cattatcaca gcatatctca ttacatttat cgcgagtttc tgcatcctgg
      721 tgatagagat cgtagcaggt cgtatcctgg ccccctttgt aggcgtttcg ctttacacat
      781 ggacaagtat catcggcgtg gtactgaccg ggatcagcat aggtgcgtat ctgggcggaa
      841 agatggcaga caggtttccc gaaaggaaga cccttgggtg gctcctgctc ctctcgggga
      901 ttaccaccct ttgcattgcg ccgatgacta acctcgttgc aggctaccat ttccccacca
      961 cccttatgct gcgcatcctt attgtgacca ccctcacctt tttcattccc agttgcatcc
     1021 tcggcatgat atcccctgtg gtggtgaaac tgaccctcag ggatatgaag actgcgggaa
     1081 atgtggtggg aaggatctat gctgtctcca ccctgggctc catcatcggg acctttgctg
     1141 caggcttctt cctcatctcc tggatgggaa caaggcacat cattctgttg atgggtctga
     1201 tactcatcct tactgcgctg gtatggggct ctctcttcaa atcaaagaag accgtcttcg
     1261 gcctcctgat cgttattccg ctctttctca tctggggtgt ctacggtttc gcgtttaagg
     1321 ctccccttga cgcatacaca aactactaca aggaaagcga ttacttcacc ctaaaggtaa
     1381 aggaagccac tgcctctgat ggaaaaacac cgctgaaggc attggtgctg gacaacctga
     1441 cccattccta tgtgaacctt aaagacccgc tccatatcga atacaagtat gaaaagattt
     1501 atgcagaggt gctcaaatgg aggttttcaa aggatacgcc cttcaggagc ctcaccatag
     1561 ggggaggagg atataccctt ccccggtaca tggaggtttc ttatccccat gcatcaattg
     1621 atgtagtgga gatagacccg gaaattacga gggtggtgta tgactatctc gggttgtccc
     1681 ggaatacaag gatcaggacc ttcaatgagg acggccgctg gtatgtgatg aactgcaaag
     1741 agcggtatga cctcatcttc atcgatgcct ataatgacct ttccatccct taccacctca
     1801 ccacaaggga atttgcccaa atgctcaggg atattctgaa cccagaggga attatcctta
     1861 ccaacatcat tgacaatttc cagagagggt ctttcctccc ctccaccata aggacactga
     1921 gggaggtctt tggcgaaagg aacgtccacc ttatatccat aagccctcac tttgataaga
     1981 tacattccag cacctttatc gtactgaccg gaaaggatga ggtagacatc agggactttg
     2041 aatcctatct caagaatagg catgagggga aggccatatc agcggtagtg ccggaaaaca
     2101 ttttgaacga ctatctaaaa aggacttact ccgtggtgct cagggataac tacgcacctg
     2161 tggacaatct catagcccct gtttttgaag aacgcttcgg ctacaacaga aaagagcgct
     2221 aggcacaaaa gcccaaacga aaacaatcgc tcctttaacc cctcacacat tgaacctgaa
     2281 atttatgata tctccgtctt tgacctggta ggtcttgccc tccagtctca agagtccttt
     2341 ttccttggca gcatgcatgg tgccggacgc aataaagtct tcgtaaccca ccacctcggc
     2401 ccttatgaat cctctctcaa tatctgaatg tatcttcccc gcagccttat gggcatcggt
     2461 ccctttttgt attgtccatg cccttacctc gtcttccccc acggtcagaa aggagataag
     2521 acccagaagg tcatagctca catggatcag cttattgagg gcaggttcct gtatccccag
     2581 atcatcaagg aatactctgg cctcttctgc ggagagttgt gcaatctcca tctcgatctt
     2641 tccgcaaaga gaaagagatg catacggcct ggcgccagat gagcctttgt ccctgctccc
     2701 aaaaaaggtt tcagcgtctt tctgaatatt tttcaccttc tcggcattca aatcgctctc
     2761 cccgatattg agcaccacca cttcaggttt tatggagatg aactggaggg gcttcatcgc
     2821 ccttccctcg tcttcgtcaa agtgtatgtt cctgaggggt atttcctttt ccagggcttc
     2881 cctgcatttc acaaggagcc tcttttcccc ttcactgggc ttcttgccct tcctcgccga
     2941 ctcttccatc cttgcaagcc ttttttccac aaactcaagg tcgccgaata tgagctccag
     3001 ctccagagtc tcgatgtcct taaggggatt gatttcgttg agcggatggg agaccacctc
     3061 attttcaaag gccctcacca catggacgat ggcatccgcg tcttttataa gatcaaatac
     3121 ctttctgttc tgctccacgt ccccttttgt gagccccagg taatcaatat attccacagt
     3181 cgcataggtg atcttcttag gtttataaat acctgcgagc gtatccaccc tcctgtccgg
     3241 caccttcacc acgccgaagt gcggctcgcc actcactgtg ggatagaggg tggtctcgat
     3301 gttttgccct gtgagggcat taaaaacagt ggttttccct gagttggaaa gtccgatgat
     3361 ggctaacttc acgcgctacc tacatctgcc gtcttccctc aagggccttg ctcagtgtta
     3421 cctcatccgc aaattcgata tcactgccca tgggaagacc gtatgcgatt ctgctcacct
     3481 tcacgggatg ggaatcaagg tgttctcgga tatattgggc cgttatctcc ccctttgtat
     3541 tggggtttgt ggcgatgata acctcttcca cattgtttga ccggacccgt tgaatgagtt
     3601 catttatttt gagtttatca ggggtgactc cgtcaatagg ggatattgct cccagcaaga
     3661 catgataaag gccacgataa aatttcgatc gttcgatcac aaggatattg ctcggttctt
     3721 ccaccacaca tatctttttc ctgtccctgc cctcgtccct gcagatctcg cagacttcag
     3781 aatcggtgat attgaaacac tccctgcaga acctcgccct gtccttcacc tcggtgattg
     3841 attgtgcgat ggcctttgct tcgtgatccg gcatggtgag gataaaaaat gccagcctct
     3901 gggctgtctt tcttcctatg ccgggaagcc gcgtgagctc attgataaga ttctcgataa
     3961 tgccctgtgt catccgaata tatttcccag ccctccaagc cccgggatct gcaggccccc
     4021 tgtgagtttc gccatctctt cattgaccat ctgctgtgcc cttctcagtg cttcgttggc
     4081 agcagccagt atcaagtcct ggagcatctc gacgtcatcg gcatttacca catccctctc
     4141 gatctttatg gagatgagct cccctccccc gtttgccacc accgtgacca ttccgccacc
     4201 tgcggtggcc tccacggtct tggtctttgc ctctgcctgc agcctttgca tctctgtctg
     4261 cagtttttgc gcctcacgca tgatatcgcc caacatcttc ttagacatgt tctcctccat
     4321 cttccatatt gtctgtagtt ctcacatcca cgatccttcc ctcaaacagc tcaatggctt
     4381 cctttaccag gggttctgac aatatcttat ccttcagctc cctcttccgc ggaacctttt
     4441 tcttctccac ctcgatcttg actgacactg ttctctgaag tatcacggct gcgatttccc
     4501 tcaatgtttc aagattcttt ttcacagaat cggcatgtat cccggagcca ccattgaagg
     4561 taatcacaag agaatctcct tcgatcctga gggccgcacc caacagcttg catgccagcg
     4621 ggtggttaac cgcctccagc ttttgcataa acacgctctg gaattcactg tctggcgccg
     4681 gatggggagc agacagcgct tcatccgcag gaagtgcttc ttcagaagga gaaacaggaa
     4741 caggcctctc atcacaagct tctttccctt tgtgagggac aggaagcgta tgggccttct
     4801 gagcgtgttc acccaccacc ctgcctgcaa gcaatgactc aatatccttc accgcttcct
     4861 ggaccggctt caggacagtg agaaaacttg ccctaatgag tgccatttcc agtgcgatgc
     4921 gcggcgcgaa ggcgccccga acctctgact cagccctgat catctctgac agcaacaggg
     4981 tgagctggtc ttccgtggtc gtggtgagaa taccgttaat gcttcggagt tcgctttcac
     5041 ttatatctaa tatctcttca ggcttctgta ccaccttggc aatgagcagg tccctgaaat
     5101 agtgcataag atcttttgcg aaggatctga ggtcagtacc cctctcggta agctcggtga
     5161 ccttttcaat aatccccttt ctgtcacccc tgatcaccgc agcagacagt tctgacagag
     5221 acctgaagtc cgtgatcccc aagaggtcct ttacttcgga ttcctttatg tctgcagaga
     5281 aggccgctac ctgatcaagg atggtcaggg aatcccgcat gctcccttcc gaagctctcg
     5341 caatcatttc cagagcggaa tccgatatct gtatcccctc ggtatcagat attttcttaa
     5401 gccgcccctt gatcacctga ctcgaaatct tcctaaaagg gagatgctga cacctggaca
     5461 ataccgtggc cggaatcttc ctgggtacgg ttgttgccag gacaaacacc acatgggatg
     5521 gtggctcctc aagggtcttg aggagcgcgt tgaatgccgc atccgaaagc atatgggcct
     5581 cgtcgatgat ataaacctta tatcgtccac ctgcaggagc gtatttgacc cgctccctga
     5641 gatctctgat gtcattcacg ctgttatttg aggcaccgtc aatctcggtg acatcaatag
     5701 acgatccgtc tgtgatcccc ctgcaggagg cgcaggcacc gcagggcaca gaggtcggtc
     5761 ctttctcgca attcaacgcc tttgccagta ttctggctgt ggatgttttg ccaactcccc
     5821 tcgggccgga aaaaagatac gcatgggcga tctttccctg cacaatggca ttgctgagaa
     5881 tacggcttat gggttcctgc cctatgaggt cttcaaaacc cagcggtctc cattttcttg
     5941 ccaacacaag gtaactcata actctccagt acgcattcgg aaatctcgcg cagcgagtct
     6001 cgagagaaac ccgttaccgg tctcccattg ctggtttcct gcaaccgggg aacagcttgc
     6061 tgcggcacac agaaaaaaat gcttaccgtt gcttccttcc ggacctggcg ggattcacac
     6121 ccttccattg cgcagggcca ttcccaggct gcagaaaaac agaaatgttt tatccgttta
     6181 ttttagcaca gaggcaggta tatttaacac gtgcggactc tatccgtaca aatgacataa
     6241 gacaattaac atgcgcgaaa gcgtttgagc cgttcaaggg attgggcaca aaaaaaacca
     6301 caagcggaca gaacggaagt gcaggccctg aatttcacag gtgaggcatt cctctggctg
     6361 tcacatcctg caccgggaac ctgattgtta tttcttttgc tgtaattcaa aagacttctt
     6421 catagccttt ttgttctgct cacaacaggt ttgcaatttg ttgatcttgt cctgcaaagg
     6481 ctcaatctgt tgtctcacat aatcctttga cgcacatccc actgaaaatg ccaacataag
     6541 aaccacaaac atcatcctct tcattccctc tcacctcctt tttccgttaa agttatgcag
     6601 ccggtctaag aacatgttgc gaccttctgt tcccaatcaa ctatctaaaa tgtggtcgtt
     6661 tccgtggcgg agagagtggg atttgaaccc acggtgcggt attagcgcac acacgatttc
     6721 cagtcgtgct ccttcagccg ctcggacatc tctccaaagc ctaaaaatta caataaactt
     6781 attgatatta ctaaggtttc tatcactcca ctatccggcg tctcctggcg tctccaggcg
     6841 tatttttgtg tccttttgga tgcctttgca gggaatccgc aggaaatttt tatcatcttc
     6901 cctacgatcc tataaaacca cataatgaca aagatgcaat tatctttaat catcccaaca
     6961 gcgaaaccca caacaggaat ctgattcata gctcgtacag tataacataa atccccacgg
     7021 atcattctcc ctcccacatc aatctatcca tgaaggtaat atggggtaaa ttcagggtat
     7081 tgcagagtag tctgatttcg aggatgtgga gccttttagc ccccgattcc tatttactga
     7141 cgaaagacag cggcttgcag agttcttggg ccaattttga cttgtgttga gtgctgcatc
     7201 gctccaaatt aacctaatca aagcatctaa ttgtaatttt tatctttagc aaaaataatg
     7261 ccaggagtct tttcaatagg tgtgtaaaaa gccatcaggc ggccatcctt tccaacacct
     7321 ttgtttcttg agtcctgatc ccattcacaa attgttttcc ttcataaacc tcctgcaata
     7381 gccaataccc cttcaacatc ctgaagcttt tctcagcaac ctgcagaagt ttccagatca
     7441 tagcagtggc attctgaatt ttcttgaatc tctttgccgc gtcagtcctc agcctgactg
     7501 acccaaaagg tgactcaaca acattcgttg tcctaagatg cacccagtgt tcatttggaa
     7561 atgaatagaa ggtcgtcatg cggtcccagt cggacagcag tttctgggtt gccttctgat
     7621 actctttttg ataacggttt acaaaaacgc atctctgagt tccgtacatt ccttgagcgt
     7681 ctcagcatac ggcacctgct tgagctgttc ggctgcttcg ccacgaattc tcttgggaaa
     7741 acagtcaagg acatttctga tcttgtggtt ccaacaccgc tgctctttgc ccctaggatg
     7801 aatctctgcc agtgccgacc atatccccaa atgcccatcg gctatcgtca atttgccgag
     7861 actgagtcct ctgccagata ggtctctcag taccgatgac caggactcct tgctctccct
     7921 gtagccgctc tcgcaggcaa ggaagacttt ctttccactg gtaagagccc cgataatgat
     7981 cagaagtgcc gctttgtcct tttccagccc tgccttcacg tatatcccgt ctgcccactg
     8041 atagactact ttcaacgacg aaaggtcccg ctgcttccac tgctcatatt caagctgcca
     8101 tttcgccttc aaccgatcaa tcgatgccga tgataatgga gctccacttc ccaacaaccc
     8161 acgcagtgca aggtcgaagt ctcctttggc cagcccgtgc aggtacaatt ccggaagcat
     8221 atcgcccaac tccctcgacc tgcgcttgaa taacggcagt actttgctct caaacccttc
     8281 ggctcccctc agacgcggcc tccggatcgt gaccgttccg ctcatcaatg cggttcaggc
     8341 ggtctacatc gtaagaagct ccctcttgct ggcaatgaca ggctccttga cggttcttga
     8401 gtctgcgcgg tatcgttcga atcatgctat cgctcaaaag gatctgtgca tgaagggcaa
     8461 agtcaggaca cgtgagatat gccctcaatg caggggcaaa ttcaaaatcg ttgagggaat
     8521 agatattttt tgcgaaagat gtaatacgcg ccctaagact tttttcattg tcttatacca
     8581 cgataaggcg aaacaacgga taagcagaga aaaagacgga cagattcttg actcatatag
     8641 aagggcgcat aggctcttag agaatatccg taaggattac gacgatgggg tgttctccct
     8701 cagtaactat ctccccgaag aaatagaaga attcaggggt cacaaattat ttccaaaatg
     8761 gctcgggact aaaaccttaa ttgcaccttc atccctgaga gaatacaagc gatacacgga
     8821 gctttacttt gtgccttact tcggaaatca ggacatgagg cagttaaacg cgggccatat
     8881 agaggacttc tttttgtcga taccgaaagc ccaggaagag catggattta gacaactctc
     8941 tttgaagtct ttgaaaaaca tctccatcgc cctgcatact ttctgtgtat ggctctatac
     9001 cagagagact atcaagaggg ttcccgtatt tcctaaaatc actcctccaa aaccaccaat
     9061 tgattggatc acaaggaacg aacaaatgaa aatactcgct ttcatcaatt ctcatcaccg
     9121 acccatcttt gaattcttat attatcatcc cgtacgcgtg ggtgaggccc gggcattgag
     9181 aagaaagcat ttcaagcttg atattatggc ggttcatgtg tgtgaggctt tcagcctaaa
     9241 ggaaaccaga tcaagaaaaa ataaagagcc ttattatctt cccattgcca agaggttcaa
     9301 tttggatgtc ttaaaggaca agttacccga agcgttcgta ttcaccaata aggcaggtag
     9361 gccctacacc tccgagggac tgaggaagat atggcatcgg gcaaccaaga aagcgggact
     9421 ccggatacaa ctgaagaacg ctaccaggca ctcaggggct tcccagtcca tcaatgcggg
     9481 gattgacctt gccacaattt caaagtcact gggtcatgct accttgcaaa tcactaagga
     9541 caaatatgcc tccatgaaca tagaacttct cagggcagtt gtggataggg ataatgtagt
     9601 tgaattcaat gaacacgttg tttctgctaa ag
//
LOCUS       DATKOU010000208        17416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4147714, whole genome shotgun sequence.
ACCESSION   DATKOU010000208 DATKOU010000000
VERSION     DATKOU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 17416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17416
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147714"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..547)
                     /locus_tag="VMU21_09695"
     CDS             complement(152..547)
                     /locus_tag="VMU21_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO77833.1"
                     /translation="MKRILLDTNAYAAFKRNDPVALEIVRSSEHIGINVVVLGELYSG
                     FKGGSREARNRKELEQFLDSPRVEAIPIDEETAEFYAKIYWDLKKKGTPIPSNDIWIA
                     ASAMRHGLALFTYDDHFKSVDGLLLRDGD"
     gene            complement(814..1221)
                     /locus_tag="VMU21_09700"
     CDS             complement(814..1221)
                     /locus_tag="VMU21_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO77834.1"
                     /translation="MTLKGAYLFDSHALLRFFQAGAGHEKVVRLLEQIRKAGARKYLN
                     AINLGEIIYSTKRGFGDQKKIEVLAHIERLGFTVLPVPNSLVFQAAEYKAQYSISYAD
                     CFALASAVEHKASVVTGDPEFRKVEHLVEIVWV"
     gene            complement(1218..1445)
                     /locus_tag="VMU21_09705"
     CDS             complement(1218..1445)
                     /locus_tag="VMU21_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HUO77835.1"
                     /translation="MATVKTLSKGQVVIPAAIRKRHHIEPGTEMQIMEYGGIIYLIPP
                     VKDPVRAACGILPARPSLAGHLLKERKGDFK"
     gene            complement(1547..1903)
                     /locus_tag="VMU21_09710"
     CDS             complement(1547..1903)
                     /locus_tag="VMU21_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO77836.1"
                     /translation="MVLVDTPVWVAHLRKGNRGLESLLNDGLAACHPFIVGELACGNL
                     KNRAEILALLQTLPPAVQAEHEDVMSFMETYHLMGKGLGYIDMHLLASAFLSNIPLWT
                     LDKKLEQTAFLLGKNY"
     gene            complement(1905..2102)
                     /locus_tag="VMU21_09715"
     CDS             complement(1905..2102)
                     /locus_tag="VMU21_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HUO77837.1"
                     /translation="MRTTLNIEDKLLDRAAKLTGVKEKTALVRLGLESLIARESARRL
                     AMLGGTEKRLQAPPRRRTTHT"
     gene            complement(2316..3473)
                     /gene="ftsZ"
                     /locus_tag="VMU21_09720"
     CDS             complement(2316..3473)
                     /gene="ftsZ"
                     /locus_tag="VMU21_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HUO77838.1"
                     /translation="MFEIDEVKGQTAKIKVVGIGGAGGNAINNMIASNLNGVEFIAVN
                     TDTQVLETSLAPVKVQIGACITKGLGAGSNPEVGKQSALEDRASIAERLEGADMVFIT
                     AGMGGGTGTGASPVVASIAREMGALTVAIVTKPFFYEAKIRALNAEEGIRELKNSVDT
                     LIVIPNDRIALVVDKKTPILKGFSVANDVLRQAVQGISDIILKPGLINVDFADVRTIM
                     QNTGKAVMGTGVGNGEGAAVEAAKKAISNPLIEESNIEGARGILFNITGGITLSLSDV
                     QDAATIIYDSAHENATIIIGNVIDPDIDDEVRITVIATGFEEKKEKVELPQIKKWTPM
                     NESLVYKASGRVLAKNLSSGHVMNIPSSDLMPYEDPLDMPTFMRQMPQRGL"
     gene            complement(3496..4752)
                     /gene="ftsA"
                     /locus_tag="VMU21_09725"
     CDS             complement(3496..4752)
                     /gene="ftsA"
                     /locus_tag="VMU21_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUO77839.1"
                     /translation="MTFGTAGNGDFAVGLDVGTTKICAICGDREGEMMRIISVGTSPS
                     HGLRKGIVVDMERTADSVKRAVNAVEDSIGKKVRSVFVGIAGGHIKGFDGHGAIAIKG
                     IVREDDVDRLLDSASAVCVPVDREVLHVIPGGFRIDGDNGIGDPLGMSGERLECRVHI
                     VTGALTSVQNLIRCCEMAGLEVSDIVLEPLASADAVVRQEEKDKGVVLVDIGGGTTDI
                     AVYRDGIVRHTAVLALGGNHITNDLSVGLGIPAEEAERIKKAFGYTMTGMADEREEVE
                     AGDAKGAMRKVPMRYITRVIEPRCEELMTLVKKEMDSVAGGVRGTPGTKPFGGVVLTG
                     GTSLLRGIDLMAKLIFRLPVRIGIPHGVENGGLVSSPEYATGVGLLGYGLEHQGRHAD
                     LWSEKTLTSGGIFERMKYWVKGYLNK"
     gene            complement(4749..5552)
                     /locus_tag="VMU21_09730"
     CDS             complement(4749..5552)
                     /locus_tag="VMU21_09730"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HUO77840.1"
                     /translation="MKRDAKTDRTPKNRKSPNLVSRKGLWKAFFRPRTLGFAVALLGI
                     CAGVFLSFRIYRASASLFPLREVVCHGNLHLGEGELKSMAGLNGGEGLFHISTRGISE
                     KLLKSPWIKNVSIRKEFPYSMLIRIDESSPFAILEMKGRSFLIDEKGRMLEEMKGAIP
                     FLPVITADPFGDRENFMETLHLARVLKDRKVATERGRVEIVAGKGPESISMVLDSVVI
                     KIGQGDYERKLDRLFELEDEIKKRAIAVDYVDLRFANRVVVKPISEVVR"
     gene            complement(5549..6463)
                     /locus_tag="VMU21_09735"
     CDS             complement(5549..6463)
                     /locus_tag="VMU21_09735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUO77841.1"
                     /translation="MVTDKRIGVLMGGISAEREVSLKSGGAVFNALKGRLYDVAAIDV
                     GHDICEVVRRERIGIAFLTLHGGYGEDGSLQGLLDVLGIPFTGSGVLASALAMDKEAS
                     KKVFLYHGVPVPPFVVVSQKAASQQLSAISFELPWVIKPATEGSSIGVSIVKERSSLS
                     MAMEKAAGFGKRIIVEKYIPGKEIQVGILNDRILGAVEVRPKLEFYSYEAKYTAGLTE
                     YILPPEVHPDILRNAEEAALSAHTALNCRGATRVDLIVDEGGNPYVLEVNTIPGMTET
                     SLLPKIAARAGFDFPLLIEEILKEAIAG"
     gene            complement(6450..7394)
                     /gene="murB"
                     /locus_tag="VMU21_09740"
     CDS             complement(6450..7394)
                     /gene="murB"
                     /locus_tag="VMU21_09740"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HUO77842.1"
                     /translation="MTKQLLDERIRGIFGEGGSAGDLFVNEPMKGHTTLRIGGSADLY
                     VIPRNLVSMRNLMVTLKDEHIPVMPLGGGSNVLISDEGVEGAVIATVSFHDIEVIEDY
                     GDEVRLFAEAGTPLQKLVNFCRGRGYKGLEGLAGIPGSVGGAVAGNSGSFGYEIGQKV
                     ESVTVIGREGKIVSIEGKSLSFTYRSSGIPDSTIVLSAILRLGKDDPHDVAKRVEGFL
                     REKTNAQPISQWSAGCVFKNPPQAHAGRLMDEAGCKGMVRGDIEVSALHANFFVNKRS
                     GKASDFLALMDDVRERVLKAFAIALEPEIRIVGRHGDR"
     gene            complement(7401..8795)
                     /gene="murC"
                     /locus_tag="VMU21_09745"
     CDS             complement(7401..8795)
                     /gene="murC"
                     /locus_tag="VMU21_09745"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HUO77843.1"
                     /translation="MFERFRTIHFVGIGGIGMSGIAEVLHNLGYEVTGSDVKESDTIA
                     RLRSLGIKVSIGHTPDNIDDAHVVVTSSAVSHENPEVAAARERSIPVIPRAEMLSELG
                     RLKYGILIAGAHGKTTTTSLIATILAHAGLDPTVVIGGKLMATGSNARLGRGDFIVAE
                     ADESDGSFLTLSPTIAVVTNIDREHMDFFRTMEALKDAFVSFINKVPFYGLSILCAEN
                     EHIREILPRIHRRYITYGFSPQTDIHAINLKKEFMSVSFHVIYKGHDMGRFSVPMPGT
                     HSVLNSLASIAVGMELKLDLQNIREALKGFRGIQRRFEFKGEERGIKFFDDYGHHPEE
                     IRATLRAAKESLGSGGGKDGRLVILFQPHRYTRTKDLLRDFGASFSDADILYVMDIYP
                     AGEKPLEGVNGEAVLNAIRDKGHRSAAYISGHDLLMETLMNALRPNDLFFTIGAGDVW
                     KIGEALLKRMKGEA"
     gene            complement(8788..9900)
                     /gene="murG"
                     /locus_tag="VMU21_09750"
     CDS             complement(8788..9900)
                     /gene="murG"
                     /locus_tag="VMU21_09750"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HUO77844.1"
                     /translation="MKIVLAGGGTGGHLFPGIAIAEEVRRRSESAEVIFIGTEKGIEA
                     SIVPREGYPIRYLGAEGVVGKSLMKKFSSGIKTAISVCEAYRLLRGLRPDAVIGLGGY
                     ASFSPVLVGSFLSIPTVIMEQNTVPGLANRILSRIADIICVTYHESISFFPKNKTYIT
                     GNPVRQGILTVRKESAFELFGLERGKFTVFIFGGSLGAKKINNAVCSVFNYIHDIREK
                     IQFLHQTGKNDYEAVRETYRKWGFKGTLAPFIHRMPEAYAVADLVISRAGATTLAELT
                     AVGRPSILVPYPHAAGHHQEQNAQRLSEMGAARIILDHELDGETLAKNVRELYENPEM
                     RTEMARSCRSLGRPDAAQRVVEIVMSLIKKREGKGV"
     gene            complement(9897..11114)
                     /gene="ftsW"
                     /locus_tag="VMU21_09755"
     CDS             complement(9897..11114)
                     /gene="ftsW"
                     /locus_tag="VMU21_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HUO77845.1"
                     /translation="MVNDCHHDRWLLVITSVLIVFGTVMIYSSTSVVTPLFAKRNITQ
                     FFYFKRHIFTVLMGVIFMYLSFRTSVETLKKIAPYLLAFSFVLLVLVFLPKIGVSAGG
                     ARRWIRLWPSTFQPSELVKLSMVLFLSWYISMPSFKTDSFSSFIIPIAMMGIFQLVFL
                     KQPDFGAAMSLCLLTFAMLVLSGTKLRYLMLLGVLLVPVLIKLLMEPYRFRRILTFVD
                     PWKDPQGSGFQLVQSFIALGSGGLTGIGLGESKQKLSFLPESHTDFIFSLVGEELGFV
                     GVLILVCLFLFLFLRGISIAHRMKDPFGYYLASGLTMMVALQALINFSVTAGLVPTKG
                     LPLPFVSYGGSSLLMNMAAVGILLRLSKGDEAKKIVDKTEVLMEKKKALMSVYGRRPV
                     ITRYGRMDGREHS"
     gene            complement(11117..12562)
                     /gene="murD"
                     /locus_tag="VMU21_09760"
     CDS             complement(11117..12562)
                     /gene="murD"
                     /locus_tag="VMU21_09760"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HUO77846.1"
                     /translation="MENNPVEIKGKRILVFGLARSGVGAANLLAGSGAEVTVTDRKPA
                     DELGEYVRRLSPSVKVCLGEYPASVNGTDLIVVSPGVPLHIEPIAKSREKGIKIIGEL
                     ELAYQMVSSQKTEFLAVTGTNGKSTTTSLLDRMLRKGGLRSMVGGNIGNALTEEITKA
                     IHTQSELKARGKGNPGGYFPDYVVVEVSSFQLESIDEFRPMAATILNITPDHMDRYHS
                     LSDYIDAKCRIFENQGRNDFLLLNADDPLTVEIIKRMRGGSPRGGEIPRIFYFSRKHA
                     VKGTYFRGGAISFDLPDVPPSFTLQPALFKIKGVHNLENAMASAAMALLAGCPPDAVA
                     ETLGEFPGLEHRLEFVRELEGIKYINDSKGTNVGAVEKSLEGFSEPIILIAGGRDKEG
                     NFAQLRPLIKEKVKALVLIGEASGKIKGALGDLTHTVQASHMKEAVETAREMARKGDV
                     VLLSPACASFDMFKDFEDRGRQFKKIVMELS"
     gene            complement(12531..13619)
                     /locus_tag="VMU21_09765"
     CDS             complement(12531..13619)
                     /locus_tag="VMU21_09765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HUO77847.1"
                     /translation="MKKEFYVHPPRRTKFKMSERKALVIVLFLFTAVCGLLSDTVYAD
                     EIRSRAAIVMEASTGRILYGKNPNLPLPPASTTKLMTAMVVLDRIPLGSAVVISEGAA
                     TISRIRAFRAGETVTVETLLNAALIKSANDAAFALAEAVAGTEEKFVELMNQKVIALG
                     MEDTRFINATGLPGYGQHTTAYDLARMLRHALRYPLIKEIINTKASRISTEEGRAIFI
                     RNSNRLLWEDESVLGGKTGYTREAKHCLVCASGQGNETVIAAILGAPSREKLWRESEA
                     LLEKGYKILQGTEEPVMHFTRADYKTSVQPASYSERISEVKEVPHKKTHKKMAKKKTK
                     SAKSGRYTKKYKSDHAYEDGKQSGGDKG"
     gene            complement(13622..14704)
                     /gene="mraY"
                     /locus_tag="VMU21_09770"
     CDS             complement(13622..14704)
                     /gene="mraY"
                     /locus_tag="VMU21_09770"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HUO77848.1"
                     /translation="MLYSLFYSLHEWISPLNVFRYITFRTAIAIMTAMIITFLFAPRV
                     VRKLRGLSVTQQIRGDGPGTHAAKSGTPTMGGILIIVSIVMTILLWGDLKNGYIWVML
                     ASLVGFGAIGLVDDYLKVVRKNHKGLRARYKFGIQIVLALLIGILISMNPKDPYADVL
                     SIPFFKKWLFDLGWFYIPFSMVVIIGSSNAVNLTDGIDGLAIGLVGIAVLANGALVYI
                     SGNKNLAQYLQVLYLPGTGELTVFCGAMLGACLGFLWYNSYPADVFMGDVGSLSLGGS
                     LGTLAVITKHEIVLAVVGGIFVIETLSVILQVASFKLTGKRIFKMAPIHHHFELKGWP
                     EPKVIVRFWIVGIMLALLSLTTLKVR"
     gene            complement(14694..16136)
                     /gene="murF"
                     /locus_tag="VMU21_09775"
     CDS             complement(14694..16136)
                     /gene="murF"
                     /locus_tag="VMU21_09775"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HUO77849.1"
                     /translation="MHSCCGEGTGIITLSEIIEATGGRVILGRADTTVFSGVSIDSRT
                     IKEGELFVALKGERFDGHEFLMNAMEKGRGAIVSTLPAVAFKGKTIVYVRNTLRSLQD
                     IAHYMRMKRGVTVIGVTGTNGKTTTKELIASILERGHRVIKNSGNLNNHIGLPLSLTR
                     IGEGDEYAVMEMGASIKGDIKELCEIAAPEYGVITNIGPGHLEGFGNLESVRDTKLEL
                     FDAVNTIVVNADDGFLMEGISQRNREDGPKVITFGIGNHADVSAKDIVLEERRSLFTL
                     CLKNAECSEIALHVNGKFNIYNALAAASVCSALGVGPGDIEKGIRSFAGVPMRLEMKE
                     LLGAKVISDVYNANPASMEEAIKELVRLRKKRAIAVLGDMFELGAYAEEAHRKLGRWM
                     SQLPVDILIAVGPMMSKAAEEFSADNGHSRRMTITAHNSSEARRILLSVCSEGDTVLI
                     KGSRGMHMEKIMDEAPGSATDFMVRGGNAL"
     gene            complement(16162..>17416)
                     /locus_tag="VMU21_09780"
     CDS             complement(16162..>17416)
                     /locus_tag="VMU21_09780"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HUO77850.1"
                     /translation="CISHNFYGRPSEDIAVIGVTGTNGKTTTSYLIKSILETWEKRAG
                     LIGTIRYIVGDAAYPALHTTPESLEFQGLLSKMASSGCGYVVAEVSSHSLSQKRVDYT
                     RFVVVVFTNLTRDHLDFHLTMADYYKAKERLFTELLPATGTAVINADDEWGRKLMKHL
                     EGRIEHRSNHGTPSLITYGIDRKADITGRDMEDSLSGISLTLSYRDKSFSVVSPLFGA
                     PNAYNILAAASAGIALGVPVEAIQEGIKKVTHIKGRLEKVDVGQDFLCLIDYAHTPDA
                     LERLLSTARAFLKKNAVPGRVIALFGCGGDRDRGKRPVMGEIATRLSDYAIITSDNPR
                     SEDPMEIITQIVSGVSGDGYRVIPNRREAIALGVEMVVPGDIFLIAGKGHEEYQEIKG
                     VRHPFSDREIAEHAIRNKMRGYRAL"
BASE COUNT         3821 a         5136 c         3985 g         4474 t
ORIGIN      
        1 tctttttctc aatcccgagt tctttcctga gtatcttcac caaagcggca ttgatgctgg
       61 tgccttcctg tttcgccttt tctttcaggg ccgtaatcgt attctcatca agtcctctga
      121 tggtcattgc tcccatggca acctccttgt ctcagtcccc atcccgcagc aggagaccgt
      181 ctacactttt gaaatgatcg tcatacgtaa aaagcgctaa tccatgccgc attgctgagg
      241 ctgcaatcca tatgtcattg cttggaatcg gggttccctt cttcttcaaa tcccagtata
      301 tctttgcgta aaactcggct gtttcctcat caataggaat agcctccacc ctcggggaat
      361 ccaaaaactg ctcaagttct tttcggttcc tggcctctct gctgccacct ttaaacccgc
      421 tgtagagttc acccaaaaca accacgttga tcccaatatg ctcggatgac ctgacgattt
      481 caagggcgac agggtcattc ctcttgaagg ctgcataggc attggtgtct agaaggattc
      541 ttttcatggt tttgtcccgt acgcctcgtt aaatgatagc acaatagtat cacgtagtgc
      601 tatcattatt caaatggcat ttgtgtaaag caaaatctct atatgcctta cacaaataac
      661 ttagtgtctg tttagcttgt cacaaaaaac ccttattttt caaaaaaact acttaaatct
      721 tggcgtgtac gaatgacaac aaatcgccct atgactgact gcacctgttt caaccgtcag
      781 gctgtgttga gtggcctgat ccatccctcc ccgtcacacc catactatct ccacaagatg
      841 ctccaccttc ctgaactccg gatcgcctgt tacaaccgac gccttgtgct caacagccga
      901 ggcgagcgcg aagcagtccg cataggagat gctgtactgg gccttgtatt ctgcagcctg
      961 gaagacaagg ctgttcggaa ccggaaggac cgtgaaaccc aggcgctcaa tgtgagcgag
     1021 cacctcgatc ttcttctggt ccccgaaccc cctctttgtc gagtatatga tctctccgag
     1081 atttatggca ttgaggtact tccgtgcgcc tgccttccgt atctgttcga ggagccgcac
     1141 gaccttctca tgtccggccc cggcctgaaa gaacctgagc agggcatgac tgtcgaagag
     1201 atacgcccct ttgagagtca tttaaagtcg cctttgcgct ctttcaggag atggcctgcg
     1261 agagacggcc tggcaggcag gatgccgcag gctgccctga ccggatcttt gacagggggg
     1321 atgagataga ttatccctcc atactccata atctgcattt ccgtccccgg ttcaatatgg
     1381 tgcctcttcc tgatcgctgc gggaatgacg acctgtcctt tggaaagggt ttttaccgtg
     1441 gccatagtat tcctaccttt ctcatattag ttatacgttt tgatagtatt gtataacctc
     1501 tcccgaccgt tgtcaattcc gcgcccgtgc cgggacgtgg gactgattaa tagttctttc
     1561 ccagcaggaa tgcagtctgc tccaatttct tatcgagcgt ccacagggga atattactca
     1621 ggaaagccga agcaagcagg tgcatatcga tgtatcccag gccttttccc ataagatgat
     1681 atgtttccat gaagctcatg acgtcttcgt gttctgcctg gacagccggg ggaagggttt
     1741 gcaggagagc aagtatctcg gccctgtttt tcaggtttcc gcaggcgagt tctcccacaa
     1801 taaagggatg acaggctgca aggccgtcat tgagcaggga ctcaagccct ctatttccct
     1861 tgcgcagatg cgctacccat accggggtgt caacaaggac cattctatgt gtgtgtcgtc
     1921 cttctgcggg gtggcgcctg cagcctcttt tcggttcctc caagcatggc gagcctcctg
     1981 gcgctttccc ttgcgatcag cgattcaagc cccagtctga ccagcgcggt cttttccttc
     2041 acgcctgtca gcttcgccgc cctgtcaagc aatttgtctt caatgttcaa tgttgttctc
     2101 atgcgctgct cctctttgta tgcatattta tatgtattaa tatgcaccat tatgtgtata
     2161 actgtcaaga gggggcaggc tgcttttata gatctggagc gtttcgaccg tcgaggaata
     2221 ttgtgctatt cactgtttac tatttactgg tgttgttctt taaagggggg ggtggggcct
     2281 ccctggggga agggaggccc cgctggcgga gggaactata gtcctctttg gggcatctgc
     2341 ctcatgaagg ttggcatatc aagggggtct tcataaggca taaggtcaga ggacgggata
     2401 ttcatcacat gacctgaact gagattcttg gcaaggaccc ttcccgaggc cttatatacc
     2461 agggactcgt tcatgggcgt ccatttcttt atctggggca gctccacctt ttccttcttc
     2521 tcctcaaatc ccgtggcaat gacggtgatc ctcacttcat cgtcaatatc agggtctatc
     2581 acatttccaa tgattatcgt tgcattctca tgggcagagt catagatgat ggtggcagca
     2641 tcctgcacat cactgaggga gagggtaata ccgcccgtaa tattaaagag gatgcccctc
     2701 gcgccctcaa tattcgactc ctcgatcagg ggatttgata tggccttctt ggcagcctcc
     2761 actgctgcac cttctccgtt tcccactcct gttcccatca ccgctttccc ggtgttctgc
     2821 atgatggtcc tcacatcagc aaagtccaca ttgataagcc ccggtttcag gattatgtct
     2881 gatatgccct ggacagcctg tctgaggaca tcgtttgcaa cgctgaatcc tttaaggatc
     2941 ggggtcttct tgtccaccac gagggcgatc ctgtcattgg gaatcacgat cagggtgtcc
     3001 acgctgttct tgagttccct gatcccctct tccgcattca aggccctgat ctttgcctca
     3061 taaaagaagg gttttgtcac aatggcaacg gtgagggccc ccatttccct tgcgatggag
     3121 gccaccacag gggatgcccc tgtaccggtc cctcctccca ttcctgcggt gataaagacc
     3181 atgtccgcgc cttccagtct ctctgcgatc gatgccctgt cctccagcgc cgactgtttt
     3241 cccacttccg gatttgatcc tgcgcccagt cccttggtaa tgcaggcccc tatttgcacc
     3301 tttacgggtg cgagggatgt ctcgagcacc tgggtgtcgg tattcactgc aatgaactcg
     3361 acgccgttga ggttggaggc tatcatattg tttatggcat tgccgcccgc acctccgatt
     3421 cccactacct tgatctttgc ggtctgtcct ttcacctcat cgatttcaaa catgtcactc
     3481 ctcctttccc tggttttatt tatttagata acccttgacc caatatttca tcctctcgaa
     3541 tatccctcct gaggtgagcg tcttctctga ccacagatca gcatgccttc cctgatgttc
     3601 aagaccgtaa ccaaggagcc ccactcccgt tgcatattcg gggctgctca ccagaccacc
     3661 gttttctacc ccgtggggta tccctatcct cacagggagc ctgaagatga gttttgccat
     3721 gaggtcaatc cccctgagca gggatgtgcc gccggtgagc accactcctc caaaaggctt
     3781 ggtgcccggt gttcctctta ctcctcctgc cacagaatcc atctctttct ttaccagtgt
     3841 cataagctct tcgcacctcg gttctatgac ccgggtgata tatctcatgg gcactttcct
     3901 catcgcgccc ttcgcatcgc ccgcttccac ctcttctctt tcatctgcca tccctgtcat
     3961 cgtataaccg aatgccttct tgatcctttc ggcttcctct gcgggaattc ccagacctac
     4021 tgagagatca ttggtgatgt ggttgccgcc caacgcaagg accgcggtgt gcctcacgat
     4081 cccgtccctg tagacggcga tatccgtggt ccctccgccg atatccacaa gaacaacccc
     4141 tttgtccttt tcctcctgac gcaccaccgc gtcggccgag gccaggggct caagcacaat
     4201 atcgctcacc tcaagccccg ccatttcaca acatcgtatg agattctgta ccgaggtcag
     4261 tgccccggta acgatatgca ccctgcattc cagcctctct ccgctcatgc ccagcgggtc
     4321 ccctatcccg ttgtctccgt caatcctgaa accacccgga attacatgga gaacctcgcg
     4381 gtcaacagga acgcagaccg cactcgcaga gtccaggagc ctgtccacat catcttccct
     4441 gactataccc tttattgcta tggcgccatg gccgtcaaat cccttaatat ggcctcctgc
     4501 gatgcccaca aaaaccgacc tcaccttctt cccgatagaa tcctccaccg cgttcaccgc
     4561 tctctttaca gagtcagcag tacgctccat gtccaccaca atgccctttc tcaacccatg
     4621 ggaaggggac gtgcccacag aaatgatcct catcatttcc ccttcccgat ccccgcaaat
     4681 cgcgcatatc tttgtggtac ccacgtcaag acccaccgca aagtctccgt tccctgctgt
     4741 gccaaaggtc atctcaccac ctcgcttatt ggctttacca ccaccctgtt cgcgaatctg
     4801 aggtcaacat aatccactgc aatggcacgc ttctttatct cgtcttccag ttcaaaaagc
     4861 ctgtcaagct tccgctcata gtccccctgg ccgatcttga tcaccacgct gtccagtacc
     4921 atggaaatac tttcaggacc tttgcctgcc acgatctcca cgcgacctct ctccgtggcg
     4981 accttcctgt ccttcagcac tctggcgaga tggagggtct ccataaaatt ctcacggtcc
     5041 ccgaatgggt cagcagtgat caccggaagg aacgggatgg cccccttcat ctcttcaagc
     5101 atcctcccct tttcatcaat gagaaaagag cggcctttca tttcgagaat ggcaaagggg
     5161 gatgactcat cgatccttat aagcatcgag taggggaact cctttcttat gctcacattc
     5221 tttatccatg gggattttag gagtttttcg gatatccctc ttgtggagat atggaagaga
     5281 ccttcccctc cattgaggcc tgccattgat ttcagttccc cctctccaag gtgcaggttt
     5341 ccgtgacaca ccacttcccg gagaggaaag aggcttgcgc ttgccctgta aatcctgaaa
     5401 ctcaggaaga cccccgcaca gatacccagg agcgccacgg caaagccaag ggtacggggc
     5461 cggaaaaagg ctttccacag ccctttccgg ctcaccaggt tcggtgattt cctgttcttc
     5521 ggtgtacggt ccgtcttcgc gtcccttttc atcctgcgat tgcctctttc aggatctctt
     5581 caatgagtaa tggaaaatca aatcctgctc ttgccgctat ctttgggaga aggctggtct
     5641 ctgtcatccc gggtatggtg ttcacctcaa gcacataggg gttcccccct tcgtccacga
     5701 tgaggtccac ccttgttgct cccctgcagt tcagggcagt atgggcagaa agggctgcct
     5761 cttccgcatt ccgcaggata tcgggatgta cctcgggggg gaggatatac tcagtgagac
     5821 ctgcggtata tttggcctcg taactgtaga attcaagctt gggcctcacc tcaaccgcac
     5881 cgagaatacg gtcattgagg atgcccacct gtatctcctt tccggggata tacttctcca
     5941 caatgatcct tttgccaaaa ccggccgctt tttccatcgc catgctcaag gaagaacgct
     6001 ccttcacgat actcaccccg atactggagc cttctgttgc cggtttaatg acccacggca
     6061 attcaaagct gatagctgac agctgttggc tggcagcctt ttggcttacc actacaaacg
     6121 gcggaacagg aaccccgtga tacaggaaga ccttttttga cgcctccttg tccatggcaa
     6181 gggcagaggc aagaacacct gaaccggtaa agggtattcc cagcacgtcc aggagcccct
     6241 gcagggagcc gtcctcgcca tatcctccgt ggagggtcaa aaaggcgatc cctatcctct
     6301 ccctccttac cacttcacag atatcatgcc ccacgtcaat ggcagccacg tcgtagagcc
     6361 gccccttcaa tgcattgaag accgcacccc cgctcttgag ggagacctcc ctttcagcgg
     6421 aaatcccgcc catcagaaca cctatcctct tatcggtcac catgtcgtcc cactatcctg
     6481 atttccggct ccaatgctat tgcaaacgcc ttcagaaccc tttctctcac gtcatccatc
     6541 agtgcgagga agtccgaggc cttaccactg cgcttgttca caaagaaatt cgcatggagc
     6601 gcgctcacct caatatcgcc ccttaccatg cccttgcatc cagcctcgtc catgagcctc
     6661 ccggcatggg cctgcggtgg gttcttgaat acgcaccctg ccgaccattg ggatatgggc
     6721 tgcgcattcg ttttttccct gagaaatcct tcgaccctct ttgccacgtc atggggatca
     6781 tccttcccca atcgcagaat cgcactgagc actatcgtac tgtcaggaat gccggatgac
     6841 cggtatgtaa aggagagaga cttgccttcg atgctcacaa tcttcccctc tctcccgatg
     6901 actgtcactg actccacctt ctgcccgatt tcatacccga aggaaccgga atttccggcc
     6961 actgcgcctc ccacagaccc cggaatgccc gcgagtcctt ccagaccttt ataccccctg
     7021 cccctgcaaa aattgactag cttctggaga ggggtccctg cctctgcaaa aagtctcacc
     7081 tcatcgccat agtcctcaat gacttcgatg tcatgaaagg agacagtggc aatgaccgca
     7141 ccctctaccc cttcgtcaga tatcagtacg ttacttcctc cgccgagggg catcaccggg
     7201 atatgttcat ccttcagggt taccatgaga ttcctcatcg agaccagatt tcttggtatc
     7261 acgtacaaat ccgcagaacc acctatcctc agggtggtat gtcctttcat cggttcattc
     7321 acaaagagat cgcccgcaga accaccttcc ccgaatatgc cccgtatcct ttcgtccagc
     7381 agctgttttg tcatctcttt ttatgcctca cccttcatcc tcttgagcaa cgcctccccg
     7441 atcttccata catcaccggc accgatggta aagaaaaggt cattgggcct gagcgcgttc
     7501 atgagcgtct ccatcaggag gtcatgaccg gaaatatacg cggcgcttct gtggcccttg
     7561 tcccttattg cgttcagaac cgcctcccca ttcactccct ccaggggctt ttctcccgca
     7621 ggatagatat ccatcacata gaggatgtcc gcgtcagaaa aagaagcacc gaagtcacgg
     7681 agaaggtcct ttgttcttgt gtagcgatgg ggttgaaaga gtatgaccag tctgccgtcc
     7741 ttcccccccc cgctcccgag ggactccttt gcggcccgca acgtggcccg tatctcttcc
     7801 ggatgatgac catagtcatc aaagaacttg atccccctct cctcgccctt gaactcgaat
     7861 ctcctctgta tcccgcggaa tcccttgagc gcctctcgaa tgttctggag gtcgagcttg
     7921 agttccatcc ccacggcgat actggcaagg ctgttaagga cactgtgcgt gcccggcatg
     7981 gggaccgaga accttcccat atcgtgtccc ttatatatca catgaaagct tacggacatg
     8041 aattcctttt tcagattgat tgcatggata tctgtctgcg gagagaagcc ataggtgata
     8101 tatctcctgt ggatcctggg cagtatttcc ctgatatgct cattctccgc gcagagtatg
     8161 gaaagcccgt aaaaggggac cttgttgatg aacgagacaa aggcgtcttt cagggcttcc
     8221 atagtcctga aaaaatccat atgttccctg tcgatgttgg tgaccaccgc aatggtgggc
     8281 gagagggtga ggaaagaacc gtcactctcg tcagcctcgg caacaatgaa atcccccctg
     8341 ccgagtcttg cattgcttcc tgttgccatg agcttgccgc cgatcaccac cgtgggatca
     8401 aggccggcat gggcaagaat cgtcgcaatg agggaggtcg tggtggtctt tccatgcgct
     8461 cccgcgatca gtatcccgta tttcaatctc cccagttcag agagcatttc ggccctgggg
     8521 attacgggga tggatctctc cctcgcggcc gctacctcag ggttttcatg ggaaaccgca
     8581 gaggatgtga ccaccacatg ggcatcatcg atattgtcag gggtatggcc tatggacacc
     8641 tttatcccga gactcctcag ccgggctatg gtatcggact ccttcacatc agagccggtc
     8701 acttcatagc cgaggttatg gagcacctca gcgatcccgc tcatccctat gcccccgatg
     8761 cccacgaaat ggatagtcct gaaacgctca aacacctttc ccctccctct tcttgatcag
     8821 gctcatcaca atctccacga ccctctgtgc cgcgtcaggc ctccccagtg acctgcatga
     8881 ccttgccatc tctgtcctca tctcaggatt ctcatagagc tctctcacat tttttgcgag
     8941 cgtttctccg tccagttcat gatcgaggat aatcctggca gcacccattt cagaaagtct
     9001 ctgcgcgttc tgttcctggt ggtgacccgc tgcatggggg tagggaacga gtattgaagg
     9061 cctccccacc gcggtcaatt cagcgagggt ggtggcgcct gccctcgata tcacaaggtc
     9121 tgccactgca tacgcctccg gcatccggtg gataaacggg gcaagcgttc ccttaaatcc
     9181 ccacttcctg taggtctccc tcactgcttc gtagtcgttc tttcctgtct ggtggaggaa
     9241 ctgtatcttt tcccttatat cgtggatata attgaacacg ctgcataccg cgttattgat
     9301 tttctttgcg ccaagactcc ctccgaatat aaagaccgtg aacttccccc tttcaagacc
     9361 gaagagttca aaggctgatt ccttacgcac agtgagaatg ccctgccgaa ctgggttccc
     9421 tgtgatgtag gtcttgttct tcggaaaaaa agagatactc tcatggtagg tgacacagat
     9481 gatatcggcg atcctgctga ggatcctgtt ggcaagaccg ggaaccgtgt tttgctccat
     9541 aatcaccgtg ggtatcgaaa gaaacgaccc caccagcaca ggactaaagg acgcatagcc
     9601 cccaagccct attactgcgt ccggtcttaa tcccctcagc agcctgtatg cttcgcagac
     9661 tgaaatggct gtttttattc cggaactgaa ttttttcatc aatgacttcc ccaccactcc
     9721 ctcggcaccc aaataccgta tgggatatcc ctcccttggt acgattgagg cctcaatccc
     9781 tttctccgtt ccgatgaata tcacctccgc cgactcactc ctccttctga cctcttccgc
     9841 aatggcgata ccagggaaga gatgtcctcc tgtcccaccg cctgcgagta ctatcttcat
     9901 gaatgctccc ggccatccat cctcccatat cgggttatca caggtcttcg gccatacaca
     9961 gacatcagcg cctttttctt ctccataagg acctctgtct tgtccacgat cttctttgcc
    10021 tcgtctccct tcgacaacct gagcagtatt cccactgccg ccatattcat caaaagggat
    10081 gaaccgccgt aactgacaaa gggaaggggg agcccctttg tgggaacaag tcccgcggta
    10141 acactaaagt tgatgagcgc ctgaagcgcg accatcatcg taagcccgga ggcaaggtaa
    10201 tacccgaagg ggtccttcat cctgtgtgca atggaaatgc ccctcaggaa caaaaatagg
    10261 aaaaggcaga caaggatcag gaccccgaca aatccgagtt cctcacccac aagggaaaag
    10321 atgaagtccg tatgggattc cggcaaaaaa gaaagcttct gtttgctctc tcccaatccg
    10381 atgccggtga ggccaccact tccaagggca ataaaggact gcacaagctg gaatcctgac
    10441 ccctgggggt ccttccacgg atcaacaaat gtgagtatcc tcctgaatcg gtacggttcc
    10501 ataaggagtt ttatcaggac gggaacgagg agaactccga ggagcataag gtaccgcaat
    10561 tttgtccctg acagtacaag catggcaaaa gtgagcagac agaggctcat ggctgcgccg
    10621 aaatcaggct gtttgagaaa taccagctga aagattccca tcatcgcaat gggtatgatg
    10681 aatgaagaga aactgtccgt tttgaacgag ggcatggata tataccatga aaggaacagc
    10741 accattgaaa gtttcaccag ttctgacggc tgaaacgtcg acggccagag tcgtatccac
    10801 ctccgtgcac cgcctgcgct cacccctatt ttcggcagaa agacaagcac aaggagaaca
    10861 aaagagaagg cgagcaggta aggtgcgatc tttttgaggg tctcgacact cgttctgaag
    10921 gaaagataca taaatatgac gcccatgagc accgtgaaga tatgtctctt gaagtaaaaa
    10981 aactgggtga tattcctttt tgcgaaaagg ggggtcacca cagaggtgga gctgtagatc
    11041 atgacagtgc cgaacacgat gagcaccgag gtgatcacca gaagccaccg gtcatggtga
    11101 cagtcattca ccatctctac gataactcca taacgatttt cttgaattgc cttcctctgt
    11161 cttcaaaatc cttaaacata tcaaaactcg cacacgcggg cgaaaggaga accacatcgc
    11221 ccttccgcgc catctctcgt gcggtctcca ccgcctcttt catgtgagag gcctgcaccg
    11281 tatgcgtcag gtcgcccagt gctcccttaa tcttgcctga tgcctcaccg atgaggacca
    11341 gtgctttcac tttctcctta atgaggggtc tcaactgcgc aaaatttccc tccttatctc
    11401 ttcctcccgc aatgaggatt atgggttcgg aaaatccctc cagggatttc tccactgctc
    11461 ccacatttgt gccctttgaa tcatttatat acttaatccc ttccaactcc ctcacaaact
    11521 ccagtctgtg ttcaagtccc gggaactccc cgagggtttc agccactgca tcaggaggac
    11581 atcctgcaag gagagccatg gccgctgagg ccatggcatt ctccaggtta tggacccctt
    11641 taatcttgaa caatgcgggc tgaagggtaa acgagggggg cacatcgggc aggtcaaaag
    11701 aaatggctcc tcccctgaaa tatgttcctt tcaccgcgtg tttccggctg aaataaaata
    11761 tccggggaat ttccccgcct ctagggctgc ctccccgcat cctttttatg atttccactg
    11821 tcagagggtc atctgcattc aagaggagga aatcgttacg tccctggttc tcgaatatcc
    11881 tgcacttggc atcaatataa tcggagaggg aatggtatct gtccatatga tccggggtga
    11941 tgttaaggat tgttgctgcc atgggcctga actcatctat ggattcgagc tgaaagctcg
    12001 atacctcaac gaccacgtag tcaggaaaat atccaccggg attccctttt cccctggcct
    12061 tgagctctga ctgtgtgtgt atcgccttcg taatctcctc ggtcaatgca ttgccgatat
    12121 tcccgcccac catactcctg agcccgccct tcctgagcat ccggtcaagg aggcttgtgg
    12181 tagtggattt tccgtttgtc cctgtcactg caaggaattc tgttttttga ctactgacca
    12241 tttgatatgc cagttcaagc tccccgatta tctttatgcc cttttcacgc gactttgcga
    12301 tgggctcgat gtggaggggc acaccgggac tcactacgat caggtcggtc ccgttcacgg
    12361 aggctggata ttcgcctagg cagaccttca cggaagggga aagccggcgg acatattctc
    12421 ccagttcatc cgcaggtttc ctgtcagtga ctgtaacctc agcccctgac cctgcaagga
    12481 gattcgctgc ccccacgccg ctcctcgcca gaccgaacac gagaattctt ttacccttta
    12541 tctccaccgg attgttttcc atcctcatac gcatgatccg atttgtattt cttggtatat
    12601 ctgccggact tcgccgactt cgttttcttt tttgccatct tcttatgtgt ctttttgtgc
    12661 ggcacttcct tcacctctga tatcctctca ctgtacgacg cgggctgaac agacgttttg
    12721 tagtccgcgc gggtaaaatg catcacaggc tcttccgttc cctgcaggat tttatacccc
    12781 ttttcaagga gcgcttctga ttccctccat aatttctccc tgctcggcgc acccaggatc
    12841 gcggcaatta ccgtttcatt gccctgcccg ctcgcacaca cgaggcagtg cttggcttct
    12901 ctggtatacc cggtcttccc gccaagaacc gactcgtctt cccacagaag ccggttgctg
    12961 ttcctgatga atattgccct cccctcttcc gtggagatac ggctcgcctt tgtattgatt
    13021 atctccttta tgaggggata tctgagggca tgcctcaaca tccttgcaag atcatacgca
    13081 gtggtatgct gcccataccc cggaagcccg gtggcattga taaaacgcgt atcctccatt
    13141 cccagcgcga tcactttctg gttcatcagt tcaacaaact tctcctctgt tccggctacc
    13201 gcttctgcga gggcgaacgc ggcatcattc gcggacttta tgagtgcagc gttcaggagc
    13261 gtctcgaccg tcactgtttc gcctgcccga aacgccctta tgcgtgagat tgtcgcagct
    13321 ccctcgctta tgactaccgc actgccaagg ggtatcctgt caagtaccac catggcggtc
    13381 ataagttttg tggtactcgc aggaggcaat ggaagattgg ggttcttccc gtagagaatc
    13441 cttccggtag aggcctccat cacaatggcc gcacgcgaac ggatctcgtc agcatagacg
    13501 gtatcggaca ggagaccgca gactgcggtg aagagaaaga gcacaataac caatgccttt
    13561 ctctcactca tcttgaattt tgtccgcctc ggcggatgaa cgtaaaactc ttttttcata
    13621 ctcacctcac ctttaatgtt gtaaggctca agagcgccag cattatcccc acaatccaaa
    13681 acctgacaat caccttgggt tcaggccatc cctttaattc gaagtggtga tggatgggcg
    13741 ccatcttgaa aatccttttt cctgtaagct taaatgatgc cacctgaagg attacggaga
    13801 gggtctctat cacaaagatt cctcccacga ccgcgagcac gatctcgtgt tttgtaatta
    13861 ccgcaagcgt gcccagtgat ccgccgagac tgagggaccc cacgtcaccc ataaacacgt
    13921 cagccgggta cgagttatac cagagaaacc cgaggcatgc accgagcatc gctccgcaaa
    13981 agacggtcaa ctcaccggtg ccgggaagat aaaggacctg gagatactgt gcaaggtttt
    14041 tgttcccgga gatatacacc aaggcaccat tggcgagcac cgcgatcccc actaacccga
    14101 tggcgagacc atcgatcccg tcagtgaggt ttaccgcatt ggaagaaccg ataataacca
    14161 ccatggaaaa gggtatgtag aaccatccga ggtcgaagag ccactttttg aaaaaaggga
    14221 tgctcaggac atcggcgtag gggtccttcg ggttcataga gatgagtatt ccgatcagga
    14281 gggcgaggac tatctggatg ccgaatttat acctggcgcg gagcccttta tggttctttc
    14341 tcaccacctt aaggtaatca tcaacaagac cgattgcccc aaatccgaca agggatgcga
    14401 gcatcaccca tatatagcca tttttcaggt ccccccacag gaggatggtc atcacgattg
    14461 agacaatgat gaggatccct cccatggtcg gcgttcctga cttggcagca tgggtgcccg
    14521 gaccatcacc ccgtatctgc tgggtgacgc tcagtcctct cagttttctt actacccggg
    14581 gggcaaagag aaaggtgata atcatggcag tcattatggc aatggcagtt ctgaaggtga
    14641 tgtatctgaa tacgttcaac ggagaaatcc actcatggag actgtagaaa agactataga
    14701 gcatttccac cccgaaccat gaaatcagtg gcgctgcccg gcgcctcgtc cattatcttt
    14761 tccatatgca tacccctgga accttttatc agcacggtat ctccctcact gcagacgctg
    14821 aggaggatcc ttcgcgcctc ggaggagttg tgtgccgtta tggtcatcct ccgagaatgg
    14881 ccgttatccg ctgaaaattc ctcagctgcc ttcgacatca ttggacccac cgcaatgagt
    14941 atatccactg ggagttgtga catccatctt cccagtttcc tgtgcgcctc ttccgcgtac
    15001 gctcccagtt cgaacatatc accgagcact gcaatcgccc gctttttcct tagccttacc
    15061 agttctttta tcgcctcttc catagatgca gggtttgcgt tgtatacatc gctgatgacc
    15121 tttgcaccga ggagttcctt catttcaagc ctcatgggaa cgcccgcaaa agagcgtatt
    15181 cctttctcga tatccccggg tcccaccccc agcgcactgc acacagaggc tgcggcaagg
    15241 gcattataga tattaaactt cccgttcaca tgcaacgcaa tctcactaca ctcagcgttc
    15301 ttcaggcaga gggtgaagag ggagcgcctt tcttcgagga caatatcctt tgcagagaca
    15361 tccgcatgat tgccgatacc gaacgtgata actttcggtc cgtcctccct gttcctttgc
    15421 gagattccct ccatgagaaa gccatcatcc gcattcacta cgatggtgtt caccgcatca
    15481 aagagctcga gttttgtatc tctcacgctc tcgagatttc cgaatccttc caaatgaccg
    15541 ggaccgatat tggtgatgac cccgtattcc ggagcagcaa tttcgcacag ctcttttata
    15601 tcgcccttga tacttgcgcc catctccata accgcatatt cgtctccctc gccaattctt
    15661 gtcagactca atgggagtcc aatatggtta ttgagattcc ccgagttttt tatcaccctg
    15721 tgccctctct ccagtatcga tgcgatgagc tccttggtag tcgtctttcc attggtgccg
    15781 gtgacgccga tcacggtgac ccctcttttc atcctcatgt aatgggcaat gtcctggagg
    15841 gacctcaggg tattcctcac atacacaatg gtctttccct taaaggcaac cgccggtagg
    15901 gtgctcacga tcgctcccct tcccttctcc atggcattca taagaaactc gtggccgtcg
    15961 aatctctcgc ccttgagggc aacaaagagt tccccttcct taatagtccg cgagtctatg
    16021 gataccccgg aaaagaccgt ggtgtcagcg cgtcctaaga tgaccctccc ccctgttgct
    16081 tcgatgatct cgctaagcgt tattattccc gtaccctccc cgcagcatga atgcacaatc
    16141 cacacaccat ggcccgtttc ttcaaagggc tctgtatcct ctcattttgt ttcttatcgc
    16201 gtgctccgcg atttcccggt cactgaacgg atggcgtacc cccttaattt cctggtactc
    16261 ttcatgtccc ttgcccgcaa tgaggaagat gtctcctggc acaaccattt ccacccccaa
    16321 agcgatggct tcccttctgt tggggatgac acgatacccg tccccggata cgccggacac
    16381 gatctgcgtt atgatttcca tagggtcttc actgcggggg ttatcagaag ttattatggc
    16441 ataatcgctc agcctcgtgg caatttcacc cataaccggc cttttccccc tgtcccgatc
    16501 tcccccgcat ccgaagagag cgatcacccg gcccggcacc gcattttttt tcaaaaatgc
    16561 cctggcagtg gaaagaagtc tctcaagggc atcaggggta tgggcatagt caatgaggca
    16621 taggaagtcc tgtcccacgt ccaccttttc cagccttccc tttatgtggg taaccttctt
    16681 gatcccctcc tggattgctt ccacaggaac acccagtgca atacccgcag acgctgccgc
    16741 aaggatgtta tatgcgttcg gggcgccgaa caacggagaa acgacactga aggatttgtc
    16801 cctgtacgag agggtaaggg aaatccctga gagtgaatcc tccatatccc tccccgtgat
    16861 atcagctttc ctgtctattc cgtaggtaat gagggaggga gttccatggt ttgagcggtg
    16921 ttcaattcgc ccttcaagat gcttcattaa cttcctcccc cattcgtcgt ccgcattaat
    16981 gaccgcggtc cctgttgcgg gaagaagttc tgtgaataac cgctcttttg ccttatagta
    17041 gtctgccatt gtcaagtgga agtccagatg gtctctcgtg agattggtga acaccaccac
    17101 tacaaatcgg gtataatcta ccctcttctg cgaaagggaa tgggatgaga cctcggcaac
    17161 cacatacccg cagcctgatg atgccatctt ggaaaggaga ccctgaaact ccagggactc
    17221 cggggtagta tggagcgcag gataggctgc gtccccgaca atgtacctta tcgtaccgat
    17281 gaggcccgcc ctcttttccc aggtctcaag tattgatttg atgagatagc ttgtggtggt
    17341 ctttccattg gttcctgtga ccccgatcac cgcaatatcc tctgacggcc ggccataaaa
    17401 attatgggaa atacac
//
LOCUS       DATKOU010000209        10507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4912879, whole genome shotgun sequence.
ACCESSION   DATKOU010000209 DATKOU010000000
VERSION     DATKOU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4912879"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..478)
                     /locus_tag="VMU21_09785"
     CDS             complement(155..478)
                     /locus_tag="VMU21_09785"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HUO77851.1"
                     /translation="MSETFKKVIELIGKGEIRISNHGYDELAEDGLLVKDVMIGVRDG
                     KVVEDYPDYPKGPCVLVLQRDREGNPIHVVWGISKGTSSPTVLVTAYKPDRSIWSDDY
                     MERRK"
     gene            complement(572..2236)
                     /locus_tag="VMU21_09790"
     CDS             complement(572..2236)
                     /locus_tag="VMU21_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HUO77852.1"
                     /translation="MKKKILINAVQPEEKRVAIVEGDTLVDFYVEVASKEHLKGNIYK
                     GVITRIEPAIQAVFVDFGSRKDGFLQIREVMPRYFQHREEGKRIRVQEVLSKGQELIV
                     QVEKDERDTKGASLTTYLSLAGRYIVMMPGQERVGVSRKIESQEDRDRLKELFTALKP
                     PENMGFILRTAGIDRSKQELSADLKYLTKLWHKIESEGKKVAAPALIYREQDIAVRTV
                     RDYLTSEVTEVLVDDEDALKNVKDFLRKTLPWRRINVKHYKEKKPLFLKHGIEEQIAK
                     INERYLYLPSKGYVVFDKTEALTAIDVNSGRSRKDETVESTALRTNLEAADEIARQMR
                     LRDIGGLVVIDFIDMESSKNRREVEERLKNAMSSDKAHSETTGISKFGILEITRERMR
                     PPYLESIQRKCEHCGGAGVVRTDEMVGVSLLRDIQARASKGGIKGIHCTLPVESMNFI
                     VNTKREDLAMTEKEFGVKIILTADAKQLPGQYKIEEEKEEEIQIEEPEKKEEKKEKAE
                     HPVKKKRFRKRRRFEKHVKTETSETPEETENTGMAVQIETPEEDGG"
     gene            complement(2233..2547)
                     /locus_tag="VMU21_09795"
     CDS             complement(2233..2547)
                     /locus_tag="VMU21_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77853.1"
                     /translation="MEEVNELIKRYGLEEDLEHVIIPIHDKDGHTKRCYLLKRRFMRI
                     VYPGEHYVDYPLAEVIEATVRYPDLLLSDALYLMHKESGAGYEGVPVDTKVREQFDQG
                     EK"
     gene            complement(2657..3127)
                     /locus_tag="VMU21_09800"
     CDS             complement(2657..3127)
                     /locus_tag="VMU21_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HUO77854.1"
                     /translation="MFTLDIPGFGPVSLEHLVSDFTGTLSVDGRLLPGVKDRLNKIAE
                     FLKIHILTADTFGKVRVALRGVRCEISILEGKRHDIQKEKYLNTLGAERVMALGNGKN
                     DRSMLRAAKIGVAVCLEEGCAIEAITSADILVSSPVDALDLLLKPKRCKATLRF"
     gene            3302..4666
                     /locus_tag="VMU21_09805"
     CDS             3302..4666
                     /locus_tag="VMU21_09805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO77855.1"
                     /translation="MNKMNRILIVDDDELMRNTLRDILEANGYPVMVASGGRQAVELF
                     RKEPPSATLLDLRMPEMDGIQTMQELKKLDPDVPIIFVTAHAEIPAAVDAIKRGAYDF
                     IVKPPQYDRLMFTLERAIEKLRLTKEIKRLNTAVDTSLESTLGRSQGMARIIEQIRQV
                     AKSDFSVVLQGETGTGKSIVARTIHDLSERAKRPFVRVDMGVIPEPLAESELFGYERG
                     AFTGAERKKKGFFEVAHGGTIFIDEMENMPPYVQSKLLSVVEEKRIYPLGDTTPLPIE
                     IRIIAASNKNIKQSVLEGTFREDLFFRLSEFMITIPPLRDRTDDIPFLAERFLREASA
                     ELHKKVEDLSEDMLNLLIRYPWPGNIRELKNVIRRAVLLSEEGAIMPEHIEFLIEDES
                     MDHGPKPLMPLKELSAMVLMDVEKKAIKQALEHSQGNKTKAASILQVDYKTLLTKIKQ
                     YNIH"
     gene            4837..5205
                     /locus_tag="VMU21_09810"
     CDS             4837..5205
                     /locus_tag="VMU21_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77856.1"
                     /translation="MKTVLIVDKSTAMRQLLSFALKDAGYWVVSAASREDAVDTLDGT
                     RIEMVISGLNENKEEMAFIREVRSKAEYRLTPIVILTADSRERRSHRATKIGASAFIM
                     KPFTPKQLVDVVRQFSRSSF"
     gene            5225..5563
                     /locus_tag="VMU21_09815"
     CDS             5225..5563
                     /locus_tag="VMU21_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77857.1"
                     /translation="MEVQMLCNAFVNEVVTQITDIREQYLQMERPPYEKELLDTVFKA
                     AETMKKPETFSLLTQFLILFMQPKRFLTLCSGKDSPFTNQPSLSFLIRPASSRMPWAV
                     CLPQLSPDPR"
     gene            5662..7137
                     /gene="lysS"
                     /locus_tag="VMU21_09820"
     CDS             5662..7137
                     /gene="lysS"
                     /locus_tag="VMU21_09820"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HUO77858.1"
                     /translation="MEELNEQIEQRHKTLEELRKAGIEPFGGPYEVTHYASDIMERYR
                     ETPKEILDATPVTATVAGRIVAMRDFGKASFAHIQDSSAKIQVYFRKDILGEKYGIVK
                     KLDIGDIVGITGKLFRTRTNELTLEVDDFTLLTKSLRPLPEKWHGLKDIETRYRQRYV
                     DLIVNPEARKAFEKRRIIIRAMRDFFDSRGFVEVETPMMHQIPGGATARPFKTHHNAL
                     GIDLYLRIAPELYLKRLLVGGYERVYEINKNFRNEGISTKHNPEFTMLEFYMAYKDYF
                     FLMAFTEELMTAIAMKATGSLEVQYGDTLINLTPPWPRIPMYEAMKSRGVPEEIFSDA
                     GYAKEWARKEGLDIETGASHGKIIDEIFKEKVEPELVQPTFIIDYPVELSPLAKKKKD
                     QPKLVERFELFIASREIANAFSELNDPMDQRERFFRQVEARQQGDEEAQWMDEDFVRA
                     LEYGMPPAAGEGIGIDRLTMLLTNSLSIRDVILFPQLKPEQ"
     gene            7219..8448
                     /locus_tag="VMU21_09825"
     CDS             7219..8448
                     /locus_tag="VMU21_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HUO77859.1"
                     /translation="MVLPYQIFVALRYLKSKKKHKGVSFNTAISIGGVALGVMALLVV
                     LAVMSGFHEDLQKKILGVNAHLVVLNFRGTLDNYETVIDKIKAEKEVISASPFVLGQV
                     MVSFGPKGQGVYLRGVDPALEEKTTDIAKHMKEGTIKDLDTQGGLPGIILGRELALRL
                     GVVRSDTITILSPTGEIGPLGMLPKVKSFRVVGIFEVGMFEYDSNLVVTGLKPAQEFF
                     GLKGDVTGLEVRISDIYKAGDVRGRVQKVLGSPYHVRDWMQMNKNLFSALKLEKFAMF
                     VILILIILVASFNIVSTLIMNVIEKQREIAILKTMGATNQGIMAIFVFQGFIIGLIGT
                     AIGLTGGYTLSYILNTYEIIKLPPDIYYLSHLPVKMKLSDFVSVSVSAILISFLSTIY
                     PAWQAAKLNPVEPLRYE"
     gene            complement(8472..9314)
                     /locus_tag="VMU21_09830"
     CDS             complement(8472..9314)
                     /locus_tag="VMU21_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUO77860.1"
                     /translation="MYYILSAIFLWSSLGIVIRLSGAPVHLLIFFSCLVSSCLIGLMF
                     LQQRYRKEIPRGRELSPLLIIGPISLANSFSFYYAYQNTTVASAVLTHYTAPVLVAFL
                     APIFLKERLTIRVFGAVAAATLGLWIMLDVSPQRFFSLIIEGDRTTGGILAGLFSGFC
                     YAVLVILFRVLSPSYHPLVMTFFQNLTIAGILLPFAARGASSGSFLWAYAVMGIVHST
                     IAPVLYFRGFKVVTANRAAILGYLEPVCAILLGVLFLGEGVTYKTVIGGGIILFSGYL
                     TVKG"
     gene            complement(9317..>10507)
                     /locus_tag="VMU21_09835"
     CDS             complement(9317..>10507)
                     /locus_tag="VMU21_09835"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HUO77861.1"
                     /translation="KHLMRRERNMFHAADPQDILDGRITDVYFERTLAILRAKGINPV
                     VKAEFIAKSLPDDWRWAVFAGLEEAMALMKRLSVKVRAMREGTVFYPYEPVMEIEGRY
                     QDFCVYETALLGLICQASGVATKAARFKKRAGERVVISFGARRMHPILAPMIERNAYI
                     GGCDGVSVVKSGEVIGEDPMGTMPHALILCMGSTVEAIKAYDEVLEPKLKRVALIDTF
                     LDEKFEVLNVAEALGEKLFAVRFDTPGSRRGNFFRILEECRWELDMRGYGHVRFYVSG
                     GIKEEDIGVLNPVVDGYGIGTSISNAPVVDYAMDIMEIEGKPLAKRGKWSGSKRVLRC
                     PSCGNRSIVPLKDTPPVCRCGGIAHDILVPVLDRGNPLLDMPSHKAIRDYVLSQMEGV
                     DLSG"
BASE COUNT         2721 a         2753 c         2351 g         2682 t
ORIGIN      
        1 tcttcccgac gaagtgcttc tctcacattg tccagctttt ctgcctcttc aactgaaaga
       61 taaggggacc agccttcgcc cgtttcaatg agttctatgt caacttccgc tacataattc
      121 ccctcatgca caagcttgac atggcgtctc tttttcattt tcttctctcc atataatcgt
      181 ctgaccaaat cgaacgatcg ggtttatatg cagtaaccaa aactgtcgga gaagatgttc
      241 cctttgagat accccataca acatgaatgg gattaccctc tctatctctc tggaggacaa
      301 gaacacatgg tccttttggg taatcggggt aatcttccac gacctttccg tcccttactc
      361 ctatcataac atctttcacc agtaacccat cttcagccaa ctcatcatag ccatggttgg
      421 atattctgat ttcgccttta ccaatcaatt caattacttt tttgaatgtt tcgctcacag
      481 cccattataa gtttatactg caaataacca agttaatgga tgatcttctc ccctatacca
      541 tatagcctac tgatcggctt aattctcaaa ttcatccccc gtcttcttca ggagtttcga
      601 tctgaaccgc catcccggtg ttttcagtct cttcgggtgt ttcgctagtc tcggttttga
      661 cgtgtttttc gaatcgtctg cgtttccgga aacgtttttt ctttacaggg tgttcagctt
      721 tttccttctt ctcttctttt ttctccggtt cctcgatctg tatttcctct tccttttctt
      781 cttcaatctt gtactgcccg ggcagctgct ttgcatctgc agtgaggatg atcttgaccc
      841 caaattcctt ctctgtcatt gccaggtctt ccctctttgt gttcacaatg aagttcatgc
      901 tctccacagg gagtgtgcag tgtatgcctt ttatccctcc ctttgaagcc ctggcctgga
      961 tatctctaag cagcgaaacc ccgaccattt catctgtcct caccactccg gcacccccgc
     1021 agtgttcgca tttcctttgt atcgattcaa gatagggcgg cctcattcgt tcccgcgtta
     1081 tttccaggat accgaacttc gatattccgg tcgtttcact atgggccttg tctgaactca
     1141 tggcattctt cagcctttct tccacttccc gcctgttttt cgaagattcc atatcaatga
     1201 aatcaatcac gaccagccct cctatgtccc tgagtctcat ctggcgtgca atttcatcag
     1261 cagcttcgag gtttgtcctg agggctgtgg attccacggt ctcatctttt ctggaccttc
     1321 ctgaattcac atcaatggcg gtaagggcct ctgtcttgtc aaacaccaca taccctttag
     1381 aggggagata gagatagcgt tcgttaatct tcgcgatttg ttcctcaatg ccatgtttta
     1441 agaaaagggg ctttttttct ttgtaatgct ttacatttat tctgcgccag ggaagggtct
     1501 tccttaagaa atcctttaca ttcttaagtg cgtcttcgtc atccacaagg acttccgtca
     1561 cctcggacgt gagataatcc ctcacagtcc ttactgcgat atcctgctcc ctgtaaatga
     1621 gcgcaggggc cgcgactttt ttcccttcac tttctatctt gtgccaaagt ttggtgagat
     1681 acttcaaatc agcggacaat tcctgtttgc tcctgtcaat gcccgctgtc ctgagaatga
     1741 aacccatatt ctcgggcggc ttcaaagcgg tgaagagttc cttgagacgg tccctgtctt
     1801 cctgtgattc aatctttctc gacacgccga ccctttcctg gcccggcatc atcacgatat
     1861 atcttccggc aagggaaaga tatgtggtca gactcgcacc ctttgtgtct ctctcgtcct
     1921 tttccacctg gacaataagc tcctggccct ttgataagac ctcctgcacc cttatccttt
     1981 tcccttcttc cctgtgctga aaataccggg gcatcacctc ccggatctgg agaaatccgt
     2041 ccttcctgga accgaaatcc acaaagaccg cctggattgc gggctctatt ctcgtgataa
     2101 ctcccttata gatatttccc ttcaggtgtt cctttgatgc cacctccacg tagaagtcaa
     2161 caagggtatc cccctccaca atggccaccc gcttctcttc aggctggact gcattgatca
     2221 atatcttttt tttcattttt ctccttggtc aaattgttct ctcactttcg tgtccacggg
     2281 taccccctca tagccggcac ctgattcctt atgcattaaa tagagcgcat cgctcaacag
     2341 aaggtccgga tatctgaccg tcgcctcaat cacctctgcg aggggataat ccacataatg
     2401 ctctcccggg tatacaatgc gcataaacct tcttttcagc agataacaac gtttcgtatg
     2461 gccgtcctta tcatggatag gaataatcac atgttccaga tcctcctcca agccataccg
     2521 cttgattaat tcattgacct cttccacgtt ctctgcgcca aaagccatgc caaaagtata
     2581 gtcaattcta caggataagg gtgctcttgt aaagaaacag gcttcatggc aaggggaagg
     2641 caaagaggcc caaaaatcaa aatctcaggg ttgctttaca tctcttcggc ttcagcaaaa
     2701 ggtcaagggc atccacagga gaactaacca ggatatcagc cgacgtgata gcttctattg
     2761 cacatccttc ttcaaggcag acagcaacgc cgatcttcgc ggccctaagc atgcttctgt
     2821 cattctttcc gtttcccagt gccatgaccc tttccgcacc cagcgtgttc aaatatttct
     2881 ctttctggat atcatggcgt tttccttcaa ggatactgat ctcgcacctg actcccctca
     2941 gtgcaaccct caccttcccg aatgtatctg cagtcaaaat gtgaatcttc aggaattccg
     3001 ctattttgtt taacctgtcc ttcactccgg gtaaaagtct cccgtccaca gacagggttc
     3061 ctgtaaagtc cgagacaaga tgttccaggc tcacaggacc aaacccgggt atgtcaagtg
     3121 taaacatgct cacaccccct gtcatcgaat attctgttgt ctactttaaa agtatgtcca
     3181 cactttttgc aatatcccat gaaaccttcc aggcaggaca gagccttcat ttaccactcc
     3241 cattccttca gatgttagaa tttcaccctc tattatgata atatgaccat gaggaattcc
     3301 gatgaacaaa atgaatagaa tactgatcgt ggatgatgat gagcttatga gaaacaccct
     3361 gcgggatatc ctggaagcga acggataccc cgtgatggtg gcatccggcg gaaggcaagc
     3421 tgtggagctt ttcagaaaag agcctccctc agcaaccctc cttgacctga gaatgcctga
     3481 gatggacggc atccagacca tgcaggaact gaaaaaactt gatcccgatg tcccgattat
     3541 ttttgtgacc gcccacgctg aaatccccgc tgccgtggat gccatcaagc gcggggcata
     3601 cgatttcatc gtaaaacccc cccaatacga caggctcatg ttcactctgg agagggcgat
     3661 cgaaaaactg cgtcttacca aggagataaa aagactcaac accgcagtgg acacatccct
     3721 cgagtcaacc ctgggcagga gtcagggaat ggcaaggatc atcgaacaga tccgccaggt
     3781 tgcaaaaagt gatttttctg tagtgctcca gggggagacc ggcactggaa agtccatcgt
     3841 tgccaggacc atacatgact taagcgaacg ggcaaagagg ccgtttgtga gggtagacat
     3901 gggcgtcata cctgaacccc ttgcggaaag cgagcttttc ggatatgaaa ggggggcctt
     3961 cacaggcgca gaaaggaaga aaaagggctt cttcgaggtt gcccacggcg gtacgatctt
     4021 catagatgaa atggagaata tgccccccta tgtccagagc aagttgttaa gtgtggttga
     4081 agagaagagg atctaccccc tgggtgatac taccccacta cccattgaga tacgtattat
     4141 cgccgcctcc aataagaaca tcaaacagag cgtcttggag ggaaccttca gggaagacct
     4201 cttcttccgt cttagtgaat tcatgatcac cattccccct ttaagagacc ggactgacga
     4261 catccctttc cttgcggaac gctttttgag agaggcatca gcagagttgc acaagaaggt
     4321 tgaagacctc tctgaagata tgctgaatct tttgatcagg tatccatggc ccggaaatat
     4381 acgggaatta aaaaatgtca tcaggagggc tgtccttctc tctgaagagg gagcgatcat
     4441 gccggaacat attgagttcc tcatcgaaga cgaatccatg gaccacggac caaagcctct
     4501 tatgccatta aaagaactct ctgccatggt attaatggac gtagagaaga aggcaatcaa
     4561 acaggcattg gaacattcgc agggaaacaa gacaaaggct gcgtctatcc ttcaggtaga
     4621 ctacaagacc cttctcacaa agattaagca atataacatt cactgagtat ggagggtgct
     4681 ccatagttag ggaaaccttt ccataattcc tctttcaccc atttccctca tcttccttta
     4741 aattcaacta gttccacata tcattgcatt ggcacattct ttgttatgca gtaaatgatg
     4801 tttcacggcg ccaggaatga aggcagcgtt atgggaatga aaacggtctt aatagtggat
     4861 aagtccactg caatgagaca gttgctctct tttgcattaa aggatgccgg gtactgggta
     4921 gtcagtgctg ccagcaggga agatgccgtg gataccctgg acggtactag aatagagatg
     4981 gtaatcagcg ggctcaatga aaacaaggag gaaatggctt tcataaggga ggtccgcagc
     5041 aaggcggagt acaggttaac ccccatagtc atccttaccg ctgattcacg ggaaaggaga
     5101 agccaccgag caaccaaaat cggtgcgagc gcctttatta tgaaaccctt tactccgaaa
     5161 cagctggtcg atgtggtcag acaattttct cgctcaagct tctgacatgg agaaagaaga
     5221 atacatggag gtacagatgc tttgtaatgc cttcgtgaac gaggtcgtta cccaaattac
     5281 cgacataaga gaacaatact tacaaatgga gaggccccct tatgagaagg aactgcttga
     5341 cactgtcttc aaggctgcgg agactatgaa aaaaccggag actttttccc tcctgacaca
     5401 atttctcatt ttattcatgc agccgaaacg gttcttgacc ttatgctcag ggaaagactc
     5461 acccttcacg aaccaaccat cactctcctt cttgatacgg ccagcctcat caagaatgcc
     5521 atgggccgtc tgccttccgc agctatcccc tgacccccgc tgaagaagct gttttactct
     5581 cgcccgtgtt ccgctccctg cacactcatt cttttgttat ccttgactta gtagaatttt
     5641 aaccattata ctttttacgt tttggaagaa ctcaacgaac agatagaaca gagacacaag
     5701 acccttgaag agctcaggaa agcggggata gagcctttcg gaggaccgta tgaggtgact
     5761 cattatgcct ccgatatcat ggagagatac agagaaaccc cgaaagagat cctggatgcc
     5821 acccctgtca ctgccacagt cgcgggcagg atcgtggcaa tgcgggactt cgggaaggcg
     5881 tcctttgcgc acatacaaga ctcctctgca aagatacagg tctacttcag aaaagatatt
     5941 cttggtgaga aatacggcat cgtaaaaaag ctcgatatcg gagacattgt gggtatcaca
     6001 ggcaagcttt tcaggacaag gaccaatgaa ctcacccttg aggtggatga tttcacgctc
     6061 ctcaccaagt cgctgagacc ccttcccgaa aaatggcatg gcctcaagga tatcgagacc
     6121 aggtacaggc agcgatacgt ggatctgatt gtaaaccccg aggcccgcaa ggcattcgaa
     6181 aagagaagga tcatcataag ggctatgagg gattttttcg attcgagggg gtttgttgaa
     6241 gttgaaacac ctatgatgca ccagattcct ggaggcgcaa ccgccaggcc ttttaagact
     6301 caccataatg ccctcggcat cgatttgtac ctcaggattg cgcctgagct ttacctgaaa
     6361 agactccttg tcgggggata cgagcgcgtc tatgaaatca acaagaattt cagaaatgag
     6421 ggaatatcca caaagcataa cccggaattt accatgctgg aattctatat ggcatacaag
     6481 gattactttt ttcttatggc gttcacagaa gaacttatga ccgccattgc aatgaaggca
     6541 acaggctccc tggaagtcca gtatggcgac acacttataa acctgacgcc gccatggccg
     6601 agaattccca tgtatgaagc gatgaaatcc cgcggggtgc ctgaggagat attctccgac
     6661 gcaggatacg caaaggaatg ggcaaggaaa gaggggcttg acatagagac cggggcttcc
     6721 catggaaaga tcattgatga gatattcaag gagaaggtgg aacctgaact cgttcagccc
     6781 acgtttatca tcgactatcc cgtggaactt tcccccctcg ccaaaaagaa gaaggaccag
     6841 cccaagcttg tcgagaggtt tgagctcttc atcgcatccc gcgagatcgc caatgccttc
     6901 tcagaactca atgaccccat ggaccagcgg gaacggttct tcaggcaggt tgaggccagg
     6961 caacagggtg atgaggaagc gcaatggatg gatgaggact ttgtgcgggc gctggagtac
     7021 ggtatgcccc ctgcggcagg agaagggatc ggtatcgaca ggcttaccat gctcctcacc
     7081 aattctctct ccatcaggga cgtcatactc tttccacagc tcaagcctga gcaataggac
     7141 atgcaaaatt tgacagcagg aattttcaat tgcccgatac tcgcatcttt tattccggat
     7201 gctggattct gaacgtccat ggtccttccc taccaaatat tcgttgccct gagatatctc
     7261 aagtccaaaa aaaaacataa gggcgtttcc ttcaataccg ccatctccat tgggggtgtc
     7321 gcactggggg taatggctct tcttgtggtg cttgctgtca tgagcggatt tcacgaagac
     7381 ctccagaaaa agattcttgg ggtaaatgcc catcttgtcg tactcaattt cagaggcacc
     7441 ctggacaatt atgagacggt catcgacaag ataaaggccg aaaaggaagt tatctccgct
     7501 tccccgttcg tcctcggtca ggtcatggtc tccttcggcc ctaagggaca gggggtatac
     7561 ctcaggggtg tagatcctgc attggaggaa aaaactaccg atatcgccaa acatatgaaa
     7621 gagggaacta ttaaggatct ggatacacag ggcgggctgc ctgggatcat cctcggcagg
     7681 gaacttgccc tgagactggg tgtggtgaga agcgatacca tcactatcct ttctccgaca
     7741 ggagaaatcg ggcccctggg catgctccca aaggtcaagt cattcagggt ggtgggaata
     7801 tttgaagtgg gaatgtttga gtacgattcc aatcttgtgg tgaccggctt gaaacctgcc
     7861 caggaattct tcggcctgaa gggagacgtc acagggttag aggtaaggat atccgatatt
     7921 tataaggcag gggacgtgag ggggagagtt cagaaagtcc tcgggtctcc ctaccatgtg
     7981 agggattgga tgcagatgaa caaaaacctc ttctctgctc taaaactcga aaaattcgcc
     8041 atgtttgtca ttctgatact catcatactt gttgcctcat tcaacattgt gagtaccctt
     8101 atcatgaatg tgatagaaaa acagagagag atcgcgatcc taaagacaat gggtgcaaca
     8161 aaccagggaa taatggccat cttcgtattc cagggattca ttatcggcct tataggaaca
     8221 gccattgggc tcacgggggg atacaccctc tcgtacatcc tcaacacgta cgagatcatc
     8281 aagttgcccc ccgatatcta ctatctcagc catcttccgg tgaaaatgaa actttcggat
     8341 tttgtctctg tgtcggtctc agcgattctc atcagcttcc tctccaccat ctatcctgca
     8401 tggcaggcgg caaagctcaa tcccgttgag cccctgaggt acgaataaag tatcattgat
     8461 ggctgagcat cttaaccctt caccgtaaga taccctgaaa aaagaattat accgcctccg
     8521 attaccgtct tgtacgttac tccctcaccc aaaaagagca ctcccagaag aattgcgcag
     8581 acaggttcca gatagccgag tatcgcggcc ctgttcgctg ttaccacttt gaatcccctg
     8641 aaatagagca ccggggctat ggtcgaatgg actatcccca taaccgcata ggcccagaga
     8701 aaagaaccag aagatgcacc ccttgctgcg aaaggaagga gaatgccagc gatcgtgaga
     8761 ttctggaaga atgtcatgac aagggggtga taggaagggg acaacaccct gaagagaatc
     8821 acgagtactg cgtagcagaa tccagagaag agccccgcca gtattcctcc tgtagtccta
     8881 tctccttcaa tgataaggga gaagaatcgt tggggagaga catccagcat gatccacagg
     8941 ccaagtgtgg cagctgcaac agcaccaaaa acccttatgg tcagccgctc cttgagaaaa
     9001 atgggcgcga ggaacgccac aagcaccggt gcggtataat gggttaatac ggcactcgcc
     9061 actgttgtgt tttggtacgc gtaataaaag gagaatgagt tggcgaggct tatgggtcca
     9121 ataatgagaa ggggggaaag ctcccttccc cttggtatct ccttccggta ccgttgctgc
     9181 aagaacataa gacctatgag acacgaggac acaagacagg agaaaaatat aaggagatga
     9241 accggagctc ctgaaagacg gataacaatg ccaagggagc tccagagaaa gatagcagag
     9301 aggatataat acatgtctat ccgctgaggt caactccctc catctggctc aagacatagt
     9361 ctcttattgc tttgtgagag ggcatatcca gaagagggtt tcctctatcc agaaccggga
     9421 cgagtatgtc atgagcaatt cccccacacc ggcagacagg aggagtatcc ttcaaaggaa
     9481 caatacttct attaccgcag gagggacatc tgaggacacg cttgctcccg gaccactttc
     9541 cccgctttgc aagcggctta ccctcgattt ccataatgtc catggcgtaa tcgaccacag
     9601 gtgcattgct gatggatgta ccgatgccat atccgtcaac cacggggtta agaacaccga
     9661 tatcttcctc ttttatccca ccactcacat agaatcgtac atgtccgtat cccctcatat
     9721 caagttccca cctgcactct tccaagatcc tgaagaagtt gccccgcctc gatccaggcg
     9781 tatcgaacct tacggcaaaa agcttctcgc cgagggcttc agccacattc agaacctcaa
     9841 acttttcatc aagaaaggta tcaatcaatg caaccctttt tagtttcggt tccaatacct
     9901 catcgtatgc ttttatcgct tcaacagtag accccatgca gaggatcaat gcatggggca
     9961 tggtacccat ggggtcttct ccgatgacct cccccgattt taccactgat acaccgtcac
    10021 acccgccgat ataggcgttc ctctcgatca tgggtgcaag gatggggtgc attctcctgg
    10081 caccaaagct gatcactacc ctctcccctg cccgtttctt gaagcgagcc gcctttgtcg
    10141 caacccccga agcctggcat atcagaccaa ggagagcagt ctcgtacaca cagaagtcct
    10201 gatacctccc ttctatttcc ataaccggct cataaggata aaagaccgta ccctccctca
    10261 tcgctctcac tttcacggag agacgtttca tgagtgccat agcctcctca agtccggcaa
    10321 agaccgccca tctccagtca tcgggaagac tcttggcgat gaattccgcc ttcaccacgg
    10381 ggtttattcc ctttgccctc aatatggcaa gggttctttc gaaatacaca tcggtaatcc
    10441 tgccatcaag gatatcctgc ggatccgccg catgaaacat atttctctcc cttctcataa
    10501 ggtgttt
//
LOCUS       DATKOU010000210         9273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4523613, whole genome shotgun sequence.
ACCESSION   DATKOU010000210 DATKOU010000000
VERSION     DATKOU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523613"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1343
                     /locus_tag="VMU21_09840"
     CDS             96..1343
                     /locus_tag="VMU21_09840"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HUO77862.1"
                     /translation="MKSKRISGKHAPVLGALEILKYVGPGFLVTVGFIDPGNWASNMA
                     AGSSYGYSLLWIVTLSTFMLIILQHNAAHLGIATGLCLSEASTHFFKRWISKVFLLTA
                     VFASISTAVAELLGAAIGLQMLFRIPITAGATLSALFSMYMLFSRSYKKLEKWIIVFV
                     SLIGISFLFELSMVRVEWTEALKGWITPSLPSGSLPVVMSVLGAVVMPHNIFLHSEII
                     QSRQWNLEGEHVIRKQLRLEFMDTLFAMIVGWAINSAMILMAASVFFGTSTVTDLPQA
                     QATLRPLLGNAAASIFAVALLLSGFSSSVTAAMAGGSIFAGIFREPFDPSDSHSRTGV
                     IITILGALFLIAFLKNPFQGILWSQIILSIQLPWTILALIVLTSSSRVMGGFANPVSH
                     KILLWTTALVVSLLNVMLLIDLM"
     gene            1452..1829
                     /locus_tag="VMU21_09845"
     CDS             1452..1829
                     /locus_tag="VMU21_09845"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77863.1"
                     /translation="MKLLKIIVLCMVALGLVFSSALAMQHTPEERGKTLFNDPKAFGG
                     QTACSSCHPDGKGLEKAGAKKDWTTPAGKTKTLEEAINLCIVNANKGKALDVKSDQMK
                     DMVAYIKSLGKKEAPKKPKAGGY"
     gene            1999..4020
                     /locus_tag="VMU21_09850"
     CDS             1999..4020
                     /locus_tag="VMU21_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUO77864.1"
                     /translation="MPRDIPVGNGNFLITFDQDYCLRDIYYPYVGQENHTGGHKFRFG
                     LWVEGNFSWINSREWRLTQEYAKESLLTHVTARHEQMAVQLHCNDMVDFKENIYIKAI
                     LLKNFSDKERLFRLFFHHDYHILESAAGDTAYYDPDEKAIIHYKGNRYFLMSGIRDNL
                     QGIDQYATGVKEFHGLEGTWKDAEDGTLQGDPISQGSVDSVLSFSVDVKPGEDKTLYY
                     WITVGKNYAEVSNLNRMLIKQGPEYFMRRTEGYWRAWINKEELDLSSLPQKIQEVYKL
                     SLLVIRTNIDNHGAILAANDSDIQHFARDTYSYMWPRDGAFTAYALDMAGHFGITRRF
                     FEFCLDIISEGKESGGFFLHKYNPDGCLGSSWHPWVHGGERVLPIQEDGTGLVLWASW
                     FHLNRYRDIEFAVNLYENLVIQCADFLVSYRDNKTGLPLPSYDLWEEKRGVHTFTVSA
                     VYGGIRAAEQFANFFGDTERAMAYRIAAEAIKEAMGKYLYSQEHKRFLKTLVFHKDGS
                     LEPDVTIDASLYAPFYFGVFAPRDERVVNTMNAVRDRLWIKTDVGGIARYEGDKYHRV
                     TDNIRDIPGNPWFICTLWLAQWHIAKAETLEELKEALYLIDWVCQRGLPSSVLAEQVH
                     PFTNQPLSVSPLTWSHAALVTTIVEYLDKFKRLSLCTHCGKPLSAHDNT"
     gene            4520..5563
                     /locus_tag="VMU21_09855"
     CDS             4520..5563
                     /locus_tag="VMU21_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO77865.1"
                     /translation="MSVTQQQDFFVQLHLTERCNLRCRHCYQSGTRNQELSLAEIRHV
                     AVEVSEMLDTWTDAYDVPFSPSFNITGGEPFLRQDIFEILQEVGDRGFELYLLTNGIL
                     IDKTKAQRLAELGLKGVQVSIEGPERIHEEIRGGGSFSSSLKGVRHLLDTGLTVTLNV
                     TLSDINADYFMDMVRLSSSLGVQKLGFSRLVPSGRGSGLLNNMLNKDKVSALYRSIGS
                     LNPKGLQVITGDPVASQMSDDDKDDSGSFPTGGCAAGISGLTILPDGTVTPCRRLPIS
                     IGNVRKDALREIWATSPVLESLRNKHSYKGKCGNCHRWAFCRGCRAIAYAYSQSKGEN
                     DFLDEDPQCFIDE"
     gene            5861..6439
                     /locus_tag="VMU21_09860"
     CDS             5861..6439
                     /locus_tag="VMU21_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HUO77866.1"
                     /translation="MSYTAKDYSKLIGMQGFSETLLKNHFTLYQGYVTNTNKVMDALA
                     QMLKEGKTATPEFAELKRRLGWEFNGMRLHEYYFENLGGKGEFPKGGKLERKLSEDFG
                     NYETWEKDFKGTGSMRGIGWCALYQDTTNGKLMNFWINEHDVSHPAGSNPLMIMDVFE
                     HAFMIDYGLKRADYIEAFFKNIDWKSVESRLK"
     gene            6492..6689
                     /locus_tag="VMU21_09865"
     CDS             6492..6689
                     /locus_tag="VMU21_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77867.1"
                     /translation="MFKVKTFGMEIRPLKAMHELAALDDMVNKFFVEHNIKQVVTVSD
                     VATTDDTGETIGIIRVVSYEV"
     gene            6975..7478
                     /locus_tag="VMU21_09870"
     CDS             6975..7478
                     /locus_tag="VMU21_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HUO77868.1"
                     /translation="MRVLIISADSFEDTELLVPYYRVREEGIDVHVASMTKTKIKGKH
                     GYEVEVDRTLKEINPEYYNLLLLPGGKAPEAIRKEKDAIEIARHFFHQKKPVSAICHG
                     PQILITAGLLRGRHATCYKSVAQELRDSGAIYEDKEVVVDGNLVTSRQPSDLPAFLRE
                     TIRMLKQ"
     gene            complement(7738..8979)
                     /gene="mtaB"
                     /locus_tag="VMU21_09875"
     CDS             complement(7738..8979)
                     /gene="mtaB"
                     /locus_tag="VMU21_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HUO77869.1"
                     /translation="MKVSLLTLGCKVNQAESSQIAAGLVAHGCCVVDLDGNPDFCIIN
                     TCSVTSKSDYQSRQLIRRAAKAGSKVIVTGCYSELNEEAVRSMDGVAMVVANNKKEFI
                     TNKLSNITSCKDFKIIQNVKSRHFVKVQDGCNNACSYCVIPSARGRSRSRGINEVIEE
                     INSAAEFYNETVVTGIHLGTYGYDIRPKVSLAILLKTILLKTSVKRIRLSSLEVNEID
                     DELVELIQEERVCKHLHIPLQSGDNRILRLMNRNYCISQFKDRLNSIFQKIPEISIGT
                     DIIVGFPGEGEIEFTNTKSFIEDAPISYIHVFPFSPRKGTKAFEMKPRVSDVIKKQRC
                     EILRSLGKRKKGEYMNQQVGRALDLLIEETDSDGASVGTTGNYLKARAYLDSPRLKDV
                     VYVRVDEARDGMLIGHPIGTS"
     gene            complement(8976..>9273)
                     /locus_tag="VMU21_09880"
     CDS             complement(8976..>9273)
                     /locus_tag="VMU21_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HUO77870.1"
                     /translation="GTKAATMGCQVDDNVKSARLTEILRVQDSITLKLNKRLENSIQE
                     VLVEGPGDADKDMLTGRSRSNKIVNFKGEGLSRGEVVDVKILRVRKHSLDGEAL"
BASE COUNT         2515 a         2108 c         2175 g         2475 t
ORIGIN      
        1 cagagtaata tcaaaaaagg tccgtgatat ccaaatttga acggaaacag tttttatggt
       61 agaacttaag taaaattgag ttcggagaaa cttccatgaa aagcaaaaga atctctggca
      121 aacatgcccc ggtattaggg gcattggaaa tccttaagta tgtagggccg ggatttcttg
      181 tcactgtggg atttattgat ccagggaatt gggcatccaa tatggccgca gggtcaagtt
      241 acgggtactc cctcctctgg atagtcaccc tctccacctt catgcttatc attctgcagc
      301 ataacgctgc ccatctggga attgccacag gactctgtct ttcggaggcc tccacacatt
      361 ttttcaagcg atggattagt aaagtctttc tcctcaccgc tgtctttgcc tccatatcca
      421 ctgcagtagc agaactcctg ggagcagcca taggtctcca aatgctcttc aggataccca
      481 tcaccgcagg agcaaccctt tcagctcttt tcagcatgta catgcttttt tccaggagtt
      541 acaaaaaatt ggaaaaatgg atcatcgttt ttgtgtctct cataggcatt tcattcctgt
      601 tcgaactcag tatggtaagg gtagagtgga ccgaggctct caagggctgg atcactccct
      661 ctttgccttc cggttctctc cctgttgtga tgagcgtgtt gggtgcggtc gtgatgcccc
      721 acaacatttt cctccattcc gagattattc aaagcaggca gtggaatctt gagggcgaac
      781 acgtaataag aaaacagttg agattggaat tcatggatac tctttttgcg atgattgtgg
      841 gatgggcaat aaacagtgcc atgatcctta tggctgcttc cgtcttcttc ggtaccagta
      901 ccgtaacaga cctgccccaa gcgcaagcaa ccttgcggcc gttgttggga aatgctgctg
      961 cctctatttt tgctgttgcc ctccttcttt ccggtttttc ctcctccgtc actgccgcaa
     1021 tggccggagg gagcatattt gcaggcatat tccgtgagcc ttttgacccg tctgacagcc
     1081 actcccgaac aggcgttata atcactattc tgggcgccct tttcctcata gcttttctca
     1141 agaacccgtt ccagggaatc ctctggagcc agattatttt gagtatccag cttccatgga
     1201 ccattctggc ccttattgtc ctcacttcct cttccagggt tatgggagga tttgcgaacc
     1261 ccgtatccca taagattctg ctctggacga ctgctctggt tgtttccctc ctcaatgtta
     1321 tgctccttat tgacttaatg tgaagggtta cgttctctcc ctgtctgtat tgcaagttca
     1381 aagtattttg ctaaaattgt tgtgagagga tttttttgaa tacatcataa caagaaagga
     1441 ggtgaaacag aatgaagcta ctcaagataa tcgtactctg tatggtagcg ctcgggctgg
     1501 tcttctccag cgccttggcc atgcagcata cccctgaaga aaggggaaaa actctcttca
     1561 acgatccaaa ggcattcggg ggccagacag cgtgcagttc atgccatcct gatggcaagg
     1621 gcctggaaaa ggccggcgca aaaaaggatt ggacaacacc tgcgggaaaa accaagaccc
     1681 tggaagaggc gatcaatctc tgcattgtca atgccaacaa aggaaaggcc cttgatgtaa
     1741 aatcagacca gatgaaggat atggttgcgt atatcaagtc cctgggaaag aaggaagcgc
     1801 cgaagaaacc aaaagccgga ggttattaac aagatgaggg gagagaggct cttttttgct
     1861 ctccccattt tattgatgaa catgtctgtt catttttcaa agtatctgcg acgaaagcca
     1921 atgaagaaga tactgtgtgg cttacccttg cttctgcccg tacttgagtt tcctaatcca
     1981 ttatgaggag gtaacacaat gccccgggat attccggtag gaaacggcaa cttccttatc
     2041 acctttgatc aggattattg cctcagggat atttattatc cctatgtggg acaggaaaat
     2101 cataccggtg gccacaaatt caggtttggc ctctgggttg agggaaactt ctcatggata
     2161 aacagcaggg aatggaggct cactcaggaa tatgccaagg agagcctcct gacccatgtt
     2221 accgcgagac acgagcaaat ggctgtacag ctccactgta acgacatggt cgactttaaa
     2281 gaaaatattt atattaaggc catactcctg aagaacttct ccgacaagga aaggctcttc
     2341 cgtctcttct tccaccacga ttaccacatc ctcgaatcag ccgcaggtga taccgcctac
     2401 tatgatcctg atgaaaaggc gatcattcac tacaagggta accgttattt tcttatgagc
     2461 ggaattcggg acaatcttca gggaatagac cagtatgcca caggtgtaaa agaattccac
     2521 gggctggaag gcacgtggaa ggatgcagag gacggaacgc tccagggcga tcctatatcc
     2581 cagggttctg ttgattccgt gctgtccttc tcggttgacg taaagcccgg agaagacaag
     2641 acactgtact attggattac tgtaggcaaa aattatgcgg aagtgagtaa cctgaaccgg
     2701 atgcttatca agcaaggtcc ggaatatttc atgcggcgaa ctgagggtta ctggagggca
     2761 tggatcaaca aggaggaact cgatctttcc tctctccccc aaaagataca ggaagtgtac
     2821 aagctcagcc tgctcgtcat caggacaaac atagacaatc atggcgccat acttgcagcc
     2881 aatgattcag acattcaaca tttcgcaaga gacacctaca gctatatgtg gccaagggac
     2941 ggcgccttta ccgcctatgc gctggatatg gcgggccatt tcgggatcac ccgaagattt
     3001 tttgagtttt gcctggacat tataagtgag ggaaaggaat ccggcggctt tttccttcat
     3061 aaatacaatc ctgatggttg tctcggcagt tcatggcatc cctgggtaca cgggggcgaa
     3121 agagttcttc ccatccagga agacgggaca gggcttgtgc tttgggcttc atggtttcat
     3181 ctcaacaggt atcgggacat agaatttgca gtcaatctgt atgagaatct tgtgattcag
     3241 tgcgcggatt ttcttgtttc ctacagagac aataagaccg gactaccgct cccctcttat
     3301 gacctttggg aagagaagag gggggtacac accttcaccg tttccgctgt atacggggga
     3361 ataagggcag cagagcaatt tgcaaacttc tttggagaca cggagagggc tatggcgtat
     3421 agaatcgcgg cagaggcaat aaaggaagca atgggtaagt atctctacag tcaagagcac
     3481 aagagattcc tcaagaccct tgtgttccat aaggatggct ctcttgaacc cgatgtcact
     3541 atcgatgcga gtctttatgc tccgttctac tttggggtat ttgctccaag ggatgaaagg
     3601 gttgtgaata ccatgaatgc cgtcagggac cgtctctgga taaagaccga tgtagggggc
     3661 attgcgagat atgaaggaga caagtatcac agggtgactg acaatatcag ggatatcccc
     3721 ggaaatccct ggtttatatg cacgctttgg cttgctcaat ggcacattgc caaggcggag
     3781 accctcgaag aacttaagga agcgctgtac cttatcgatt gggtgtgcca gagaggactt
     3841 ccttcttctg tccttgccga acaggtacat cctttcacca atcagcctct ctctgtctct
     3901 cctctcacat ggagccatgc agcgctggta acaacaattg tggaatacct tgataaattc
     3961 aagagattaa gcctttgcac tcattgcggg aaaccgctgt ccgcccacga caatacctag
     4021 gtaaacgatg aggaggtctt taaggaatga gaaaactagc tacgtgagtc gaggaagata
     4081 ttctctttcc tccaaatacc tgtctttagt tatgttgcgc cccgctgaca tgtcaatgcg
     4141 tcctggatct cccgagtaaa gacaagttct acttgacaaa cgaaacagag ctgttatcct
     4201 atagatgaaa cctattagtt ttctaaaagt tattgcttac aaggatggca atgcataatc
     4261 cctctaggag gaaaggaggt gggacaggat gaaaaagaca ttgagcaaaa aggcacttgt
     4321 gaaggaggct gccttctggt ctgacttcag aaaggcaatc aaagagaatc ctggcaagat
     4381 atgccacaag tgaaagagct gaaagagcta atgttgctgg tcaagcaaca agacggtcga
     4441 taagcagata cggtgatggt gagatgaaag ctttccgccc tatctgctta tttttttgtc
     4501 ctggtggtat actttagtta tgtcagtgac acagcaacaa gatttttttg tccagttaca
     4561 cctcaccgag agatgcaacc tcagatgcag gcactgctat cagtcgggca caaggaacca
     4621 ggagctttcc cttgcagaga ttcgacatgt tgcagttgaa gtatctgaga tgcttgacac
     4681 atggacggac gcctatgatg ttcctttttc acccagtttt aatattaccg gtggtgagcc
     4741 ttttctacga caggatattt tcgaaatcct tcaggaagtg ggtgacaggg gatttgagct
     4801 ttacctcctc accaacggca tccttatcga taaaacaaaa gcccaaaggc tcgctgaact
     4861 gggcttaaaa ggcgtgcagg taagcattga aggacctgag aggattcatg aggaaataag
     4921 ggggggaggg agtttttcgt cttccctcaa aggagtgcgg catctcctcg atacgggatt
     4981 gacggtcacc ctgaatgtca ccctttctga tatcaatgca gattatttta tggatatggt
     5041 gagactctca tcttcccttg gcgtccagaa acttggattc tctaggcttg taccttcagg
     5101 gaggggttcg ggactactaa ataacatgct caataaagac aaagtaagtg cactctaccg
     5161 ttcaattggt tctcttaacc ctaaagggct tcaggtcatt acaggagacc ctgtcgcatc
     5221 ccagatgtca gacgatgata aggacgattc aggctccttt cccacaggcg ggtgtgctgc
     5281 aggcatatcg ggattgacga tacttcctga cgggaccgtc accccttgca gaaggttacc
     5341 tatttcgatc ggaaatgtga gaaaggatgc gttaagggag atttgggcaa cgtcgccagt
     5401 gttggagtca ttgaggaata aacacagcta taaaggcaaa tgcgggaact gccaccgctg
     5461 ggccttctgc agaggatgca gggcaatcgc atatgcatac tcccaatcga aaggcgagaa
     5521 tgattttctt gatgaagatc cccagtgctt tatcgatgaa taacagtgcc tataaggttt
     5581 tttccaggct ctaagcaaca tcgtgccggc attgcctgcg gcatagtacc ttagagcctc
     5641 ctttgatacg ctcccagtcc tgatattgaa tatgcgggct tgacattccg cctgtctagt
     5701 ataaactaac ataagacgta tatctcctgt tttcacaatg aggtgaactc tgccgattat
     5761 agggaactga aggaggctta attctgtagc atagtggaaa tgcttatcat ttcctctggc
     5821 tgcataatcc ccagatacta aaaagaagga ggaaaaacag atgtcgtaca cagcaaagga
     5881 ttattccaaa ctcatcggga tgcaagggtt cagtgaaacg ctcctcaaga accactttac
     5941 cctgtatcag ggttatgtga caaacacaaa caaggtaatg gatgccctcg cgcagatgtt
     6001 aaaggaagga aagactgcca cccctgaatt tgccgaactc aagagaaggc taggctggga
     6061 atttaacggc atgaggcttc acgaatacta ttttgagaat cttggcggga agggagagtt
     6121 ccctaaaggg ggaaagcttg aaagaaagct ttcagaggat tttggaaact acgagacatg
     6181 ggaaaaagac tttaaaggaa caggctccat gagaggtata ggctggtgtg ctctctatca
     6241 ggatacgacc aacggaaagc tcatgaactt ctggatcaat gaacatgacg tttcacatcc
     6301 tgcaggatcc aatcccctta tgataatgga cgtatttgag catgccttca tgattgacta
     6361 cggactcaag agagctgatt acatagaggc tttctttaag aacattgact ggaagtctgt
     6421 ggaatctcgg ctcaagtgat tgaataccat aatcttgcca tgaaaaccct gggagaaaag
     6481 gaggaacagg aatgttcaag gtaaagacct ttggtatgga gattcgtcca ctcaaagcga
     6541 tgcatgagct tgcagctctt gatgatatgg tcaacaagtt tttcgttgaa cataatatca
     6601 agcaggtagt cactgtttct gatgttgcaa caacagacga cactggcgag acgataggca
     6661 ttataagggt tgtgagttac gaggtgtagc agccttttca cggctgtccg cctcagtggc
     6721 atgtctctgc cttgagccag tcaatccctc cattgcgtgg gtcgtcactg aaggatcgcg
     6781 cactcctttg cgaagaaccg gatggaggca cgaccttttc cgactccctg agggagatgg
     6841 caagaaggat gagatccctc tgggagagct tttagtgagg gcaatagaaa ttagttgcag
     6901 agtagggggg taagaccacg aggttttcca gaagcccaga ggcgaagaaa caacagaaaa
     6961 ggaggttctt ttgtatgaga gtattgatta taagcgcaga tagctttgag gatacggagc
     7021 tcctcgtccc ctattatagg gtaagggaag agggcattga tgtgcatgtg gcttccatga
     7081 caaaaacgaa gataaagggc aaacacggat acgaggtgga ggttgacagg acattaaagg
     7141 aaataaatcc tgagtactac aaccttctcc ttcttcccgg aggaaaggcc cctgaggcca
     7201 taagaaagga gaaggatgcg atcgagatcg caaggcattt ctttcatcaa aaaaagcctg
     7261 tttctgctat atgccacggg ccccaaattt taataacagc gggcctcctg aggggcagac
     7321 atgcgacatg ctataagtct gttgctcagg aactgaggga ttccggagca atatacgaag
     7381 acaaagaggt ggttgtggac ggcaacctag tgacttccag gcagccctcc gatttgccgg
     7441 cctttctcag agagactata agaatgctca agcagtaata gcctaccagc cagtgcagat
     7501 aaacagggta gttgcctaga caaggcaatc aagacatcct aggatccgct accttgtacg
     7561 ttaattctat ggatttaatt ttacataata tatcttatca gacattcaat ataaacaggt
     7621 ggtgttagca aattgttatt aaaccggttc aaaacatacc cactccttac ggcttcaagg
     7681 ccctttacgt ctttgagcag gatttaggca gaaaggaaat tgataattta caatgagtta
     7741 ggaggttcca atgggatgtc ctatcagcat gccatctctt gcctcatcta cccttacgta
     7801 gacaacatcc ttcagccttg ggctatccaa ataggccctc gcctttaggt agtttccggt
     7861 tgtgccgacg gatgcaccat cactgtccgt ctcctctata aggagatcta gggctcttcc
     7921 aacctgctga ttcatgtatt cccctttttt tcttttgcca agtgatctga gaatctcgca
     7981 tctttgcttc ttaataacgt cactaacacg gggtttcatc tcaaaggcct tagtcccctt
     8041 tcgtggcgaa aatggaaaaa catgaatata ggagatggga gcatcctcaa taaaactttt
     8101 cgtattcgta aattcaattt cgccctcgcc agggaagccc acaataatgt cagtaccgat
     8161 tgaaatttca gggatttttt gaaatattga attaagtcta tctttaaatt gcgagataca
     8221 gtaattcctg ttcatcaatc tgagtattct attgtcccca ctttgaagcg gaatatgaag
     8281 gtgcttacag actctttctt cctggatcag ttccacaagc tcgtcatcta tttcattgac
     8341 ctcgagcgag ctgagcctta ttctttttac actcgttttc agcaaaattg tttttaacaa
     8401 aatagcaagt gacactttag gtcttatatc ataaccatat gttcctaaat gtatcccagt
     8461 tacaacagtt tcattgtaaa actccgctgc agaatttatc tcctcaatta cctcatttat
     8521 tcccctgctt cttgatctgc ctctggcact agggataaca caataactgc acgcattatt
     8581 gcacccgtcc tgaaccttta caaagtgcct gcttttgacg ttttgaatta tcttgaaatc
     8641 tttacatgat gtaatattag ataacttatt agtaataaat tcttttttgt tattagcaac
     8701 taccatggct actccatcca tagatctcac cgcctcttca ttaagttctg aatagcagcc
     8761 agtaacgatg accttagaac cggccttggc tgcccttctt attagttgcc ttgactgata
     8821 gtcacttttg gaagtaactg agcaggtgtt gattatgcaa aagtctggat taccatcaag
     8881 gtctacgaca caacagccat gcgccacaag cccagctgct atctgtgagc tttctgcctg
     8941 gttgaccttg caccccagtg tcaataatga gaccttcaca aagcctctcc gtcaagggag
     9001 tgctttctaa ccctgagaat tttgacatcg accacctcgc ccctggaaag accttcccct
     9061 ttgaaattga ctattttatt agacctgctc cttcccgtaa gcatatcttt gtctgcgtca
     9121 ccaggtcctt cgacaagcac ctcctgaatt gagttctcca gtcttttatt taactttaga
     9181 gtaatggaat cttggactcg aagtatttcc gtaagcctgg cggatttcac attatcgtcc
     9241 acctggcaac ccattgtagc agcctttgtt ccc
//
LOCUS       DATKOU010000211        16339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1460991, whole genome shotgun sequence.
ACCESSION   DATKOU010000211 DATKOU010000000
VERSION     DATKOU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460991"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VMU21_09885"
     CDS             complement(<1..227)
                     /locus_tag="VMU21_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77871.1"
                     /translation="MWPHVLIPVLAVAIGMQNTCPQGWAAKTAFVTCKGSHCPMKEHK
                     PAKTADHADTKKAISNVKQTFVLNIVRPDNTT"
     gene            complement(419..934)
                     /locus_tag="VMU21_09890"
     CDS             complement(419..934)
                     /locus_tag="VMU21_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HUO77872.1"
                     /translation="MEIFTFFWTIFYINLFTIGGGYVMLPLLQREVVDHYHWLSNKEF
                     VESIAIGQVTPGPLTIMNVFIGYKIFGFWGALGAVIFSYLPSIILVTIASHYYLKFKN
                     SWIVKASFQGIRPAVIGLLAAIAITLGRASIVDVPTGLIATGGFAILVFTKVEPTFVI
                     LGSGVAGALLY"
     gene            complement(936..1481)
                     /locus_tag="VMU21_09895"
     CDS             complement(936..1481)
                     /locus_tag="VMU21_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HUO77873.1"
                     /translation="MTVADASVKNILKVFLKIGTFAFGGVYSMLAFFERELVQNRKWL
                     SQEEFAESVVMGQLTPGAPIINTGIFIGYRLKRLKGALATVAGQVLPSFVIVLILSYL
                     YIRYKEIALLKAVLKGVGAAVVALIASVVYSMSGKLLKEYKGILIAIVAFLCLAVFKL
                     NPIGLIVAAGIVGLLMSRGEQ"
     gene            complement(1495..2688)
                     /locus_tag="VMU21_09900"
     CDS             complement(1495..2688)
                     /locus_tag="VMU21_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HUO77874.1"
                     /translation="MVEKSGGVAKDPVTEAQADAGIAVAKTKNLYNRMEWAGAFGDVG
                     TLIPFVVAYITIVKMPPLGLLFMFGITLIASGLYYRTPLPIQPMKAIGAAAIAGGISP
                     AALYGSGLTTGLFWLLAGITGVLRPIAKLATKPVVRGIMLGLGLTFMVDGVNRMKTAP
                     VLAVIALVVTYLLLTNPKIPAMFMLLIIGVVSAVIMNPQIMSELAKIHIGFNLPVFSI
                     PMITWNDLVVGTLLFTIPQIPLTLGNAVIAITAENNELFPDRPVTEKKMAISQGIMNL
                     VAPLFGGIPMCHGAGGMAGHVRFGAKTGGALVILGSILILIALFFSESVSIIFKIFPN
                     AILGVILFFAGSELAIVVRDIGGKKSEFYVMIVVAAVAMWNMGVAFLVGVILDNALRR
                     EWIKN"
     gene            complement(2727..3185)
                     /locus_tag="VMU21_09905"
     CDS             complement(2727..3185)
                     /locus_tag="VMU21_09905"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HUO77875.1"
                     /translation="MKDIFLILLSVIVIAQLIVSLVSRIRQKHIRGYLDLITLTKEQK
                     RQVEEIRRDFLPKVEGIRQSLRLNRLQLNDLLFAEEPDMKTIGEKSGEISALQAELER
                     EVIGHILQEKELLSPEQKRQFCEVIRGEFDKGGLGVHGERRPGPTRNAGK"
     gene            complement(3182..3610)
                     /locus_tag="VMU21_09910"
     CDS             complement(3182..3610)
                     /locus_tag="VMU21_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HUO77876.1"
                     /translation="MNCDYCKEKLSAYIDNELSSEERFLMEEHLKTCPSCAREAETLA
                     QFGLLMGTIPEETPSLAFVQRTVDKAAVIRRRSLWNKFFLNPALSFLRNAVAFVFAHN
                     GYGIHGERSLSSYGYLRTFDDSPPGSFADVYLTVIQGGGN"
     gene            complement(3660..4226)
                     /locus_tag="VMU21_09915"
     CDS             complement(3660..4226)
                     /locus_tag="VMU21_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUO77877.1"
                     /translation="MEEPETKIMVEVAEGNLSAFKTLVELHHKPLISFIARFTGDRDS
                     AEDIAQEVFLRVFKAAKDYKPQAKFKTWLFTIATNLCLNEIRDNKSSCEFVDLSDLHE
                     QEYPIIAPEAFSPQKAAENAELSKAVCKAIRSLPENQRTAILLRQYNDFSYNEISKIM
                     GLSISAIESLIQRARQSLKKSLFPYLKK"
     gene            complement(4412..5575)
                     /locus_tag="VMU21_09920"
     CDS             complement(4412..5575)
                     /locus_tag="VMU21_09920"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HUO77878.1"
                     /translation="MRKTALLVILCLSLVAGAFTSSAEERLAYVPNEGDNNVVVVDLI
                     AEKVIKTLPTGKTPHALAFTKDGKCYVNNRGTKDLTVIDANRLEVIKTIALPATSFQL
                     ALSPDGKTLAVAYKDALKVSLIDTGTDNIMKTIPVGKEPEGAFKGAMMRHSYWSKDGR
                     FLYASDDFNYNIVKIDVADFEIKAAIALPGSNHYIHPSPDGKVLYAVNETTKTGTSVT
                     LINSDTDKIISDIPIPLEPGEKGLGHHGVFTKDGNYFFVCNEGGRTVTVIDTAKMEVV
                     KTLKVGMGAGHPSMSRDGKYIFVIHHNDNIVAVIDTAKQEVVKNILVGNGKKQAHGSY
                     FTPDGKYFYMINAEDNIMNKIDVAKMEVVSKIPVGKSAMYFGMKEGKEFPSTE"
     regulatory      complement(5974..6041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6155..6442)
                     /locus_tag="VMU21_09925"
     CDS             complement(6155..6442)
                     /locus_tag="VMU21_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77879.1"
                     /translation="MKEHEEVEQHKKLPRVGQTVKSKKYGTLWRVMEKREVWQNAPED
                     DPISHQPRLVPGIYLSYWRIKPGVLPGVGRLMGFVYTVYDDTFESNWEIVG"
     gene            complement(6656..7711)
                     /locus_tag="VMU21_09930"
     CDS             complement(6656..7711)
                     /locus_tag="VMU21_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77880.1"
                     /translation="MSKIRFETSDTDLTLMNPFRDFSEEVHTVEVRKDPLLGDISVYN
                     PRLKDKVKFFFGDCDIGLVEKLIDDSAKNCIFCGDMIERSTPRYPPNLVPEGRIKTGE
                     AVLFPNLFPIGKYHSVIRLSKAHFLKLSEFRPDIIENGLKAAQTFVNLVYNNDPSASF
                     VAVNANYLFPAGATLVHPHLQMLITPTAYSYHDRLIRASHSYYQEHGSEFYLDLIDEE
                     KRKGERYVAQSGGWHWMTAFSPMGNNEVVAIHEGKNDFALLSESDLATLAKGISRVLS
                     FYESLGHLSFNYSIFSSRDGSSQKGFRCLLKIISRQNLYPNYRNDDYFLQKLLHSELM
                     INLPEELASGLRPYFQR"
     gene            complement(7769..9040)
                     /gene="eno"
                     /locus_tag="VMU21_09935"
     CDS             complement(7769..9040)
                     /gene="eno"
                     /locus_tag="VMU21_09935"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUO77881.1"
                     /translation="MSKIRKIFAREILDSRGNPTVEVDVVLDNGILGRAAVPSGASTG
                     SKEAVELRDGDKGRFLGKGVQKAVSNVNTIIAPELIGKSPSEQKVIDNLMIKLDGTEN
                     KGRLGANAILGVSLAVAKAAAQDMGKSVFQYIGGNKACRLPIPFFNILNGGKHADNNV
                     DIQEFMIAPIGAKSFREALRMGAETYHNLKAVLKSKGLSTAVGDEGGFAPNLSSNEEA
                     IQVIITAIEKAGYKPGQDIAIVLDPAASEFYQEGKYILKADNSLLSSEQMVTYYERLI
                     SKYPIISIEDGLAEDDWKGWEVLTKQLGNKIQLTGDDIFVTNSKIVAEGIRQGIANSV
                     LIKLNQIGTLSETLETVKLAEKAGYTCVFSHRSGETEDSFLADVAVATNAGQLKTGAP
                     ARSERLAKYNQLLRIEEELGDRAVFRGDLHW"
     gene            complement(9171..9893)
                     /locus_tag="VMU21_09940"
     CDS             complement(9171..9893)
                     /locus_tag="VMU21_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77882.1"
                     /translation="MEHIAERKSLHIYGEGGTGKSALLDQVYDFLKTRGTPIPIYCRS
                     SRTLRSILLQMALFLLSHFSYLESVDKFKRVKEIQCGSDIKKLSIRALRNVVFSYISL
                     ADFCVILDHLEYVTPKINGFLTTLYEKAPVITSSKQSWELSDYSFRGRLDYCLYLIPK
                     LRLDHLRKKDALVLMEILCDNLNIDYSTKRRMFDEVFHISKGNPRAISRIFETARRPE
                     YLRDNRLNLKLIMIDSKIEDIA"
     gene            complement(9976..11760)
                     /locus_tag="VMU21_09945"
     CDS             complement(9976..11760)
                     /locus_tag="VMU21_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HUO77883.1"
                     /translation="MEKLEPATMSDYQRLKVELIRCIDDILGIGAMRGCPCEELREKI
                     ETNTFNLVVVGQFKRGKTSLINALLGEDILPVAVVPLTSVVTIMKYGDALRIVVHFNE
                     GRVAEIKTESLPEYVTERGNPKNEKDVSEVIINYPSPYLRGGVQIIDTPGVGSVYEHN
                     TDVAYRYLPKSDAALFLLSVDQPLSKAELEFLKDVREYSKRIFFLLNKADYFCEADLR
                     ESMEFSKNVLKEAMVTDIRIFPVSAKLALEGKLSGAEDIVEKSLLPQFSEVLNTFLME
                     EKGKILILSVTNNLLRLLSQARFELELEIKSLTTPLEELKEKIQTFEERKKEVLTEKS
                     DFDLLLDGEIKKIMKNVLDEDLNAYGQELRLKAEANLDEYYRENTGLSLKELHKHLEE
                     HIISEVRQAVNAWRGTEDEKLAKTFETACKRFIIKIDETVDSLLKFSSELFAVPYDAV
                     KAEALWSTKSKLYYKFTEQPVGLEIITSSLTLSLPKFIGEKIIIRKMKEYLHRVITIQ
                     LCRIGSDFEKRLTKSKLDFRWEMLQRIEATIEGIGAAIKKGMSQRSKSEKGANERKNE
                     VSDILTRLDAIKDRLMKLREALGVISRF"
     regulatory      complement(11813..11911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(11905..12621)
                     /locus_tag="VMU21_09950"
     CDS             complement(11905..12621)
                     /locus_tag="VMU21_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HUO77884.1"
                     /translation="MVTKGPAKKLTIYVDEMDKFHGKPVYEVLMDIFFKKKIGGASVF
                     RGIAGYGTDGIFHTAKMLELSTSMPVRIEVVDSEDMINRVLPDVYHVVEKGLVEVADT
                     NVIKCCPKAEEKQKEQGEHMKLEGKAKMLRIIISEDDKWEGEPLYEAIVKRLIMSDIA
                     GATVYKAIAGYGPHRRYHKKKTMTVHGELPILITVIDTEDKINKVVPILDDMVGEGIV
                     VLSDVTVIKYTHKHVNPETI"
     gene            complement(12633..13007)
                     /gene="crcB"
                     /locus_tag="VMU21_09955"
     CDS             complement(12633..13007)
                     /gene="crcB"
                     /locus_tag="VMU21_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HUO77885.1"
                     /translation="MMNYLVVFAGGGIGSVARFVLATWIGQRWGRSFPLGTFVINVTG
                     SFLIGLLMTLMAERFMENPEWRLLLVVGGLGGYTTFSTFQYETGRLVADGELVYAAMN
                     IVLSTLVGFMALKLGDVIARSI"
     gene            complement(13029..13454)
                     /locus_tag="VMU21_09960"
     CDS             complement(13029..13454)
                     /locus_tag="VMU21_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77886.1"
                     /translation="MLKKILVAFDGSQQSYKAFDFALEMTKLCPGAAPEVMVISVAQP
                     PEPADIVEVDAIIDSATQHYEGLFKDLKEKAKEKNMEIKTEVVVGHPADQIVKYAKDK
                     NCDMVVVGQTGKSKIESWLLGSVSKRIATYAPCTVTIVK"
     gene            complement(13471..14664)
                     /locus_tag="VMU21_09965"
     CDS             complement(13471..14664)
                     /locus_tag="VMU21_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HUO77887.1"
                     /translation="MHEVWGMAALWIGLALIATLLSIWFRIATALSEIVVGTVAQLII
                     GAFIGTTLGSDVPWIKFLSGTGAIVLTFLAGAELDPVVFKAKLKEASAIGFISFLAPF
                     LGCTAAAYYFLHWTVQASWLAGVALSTTSVAVVYAVMLELGLNKTDFGKAVLAACFVT
                     DLGTVLALGLIFAPFTLKTLIFVGVSAAAFFILPYLTPRFFKKYGDRPSELEAKYLLL
                     FLFGLGSLAVWSDSEAVLPAYIIGMVLAGTVGKDHVLIRRLRTLTFGLLTPFYFIRAG
                     SYVSVPALIAAPVAILVLFLGKMITKIIGVYPATKTFRYAQREGIYTTLLMSTGLTFG
                     SISALFGLTHNIINQQQYSILVFVVIGSAVVPTLIANAFFIPHYLLPKREKKATLETN
                     AVNEE"
     regulatory      complement(14717..14785)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            14895..15746
                     /gene="pssA"
                     /locus_tag="VMU21_09970"
     CDS             14895..15746
                     /gene="pssA"
                     /locus_tag="VMU21_09970"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HUO77888.1"
                     /translation="MVIKHKRGGVEKQPKKGVYLLPNTLTLCGMFCGFFAILSAINGN
                     FLYAAWAIVLANVFDGLDGWIARLTNTTTRFGIELDSLSDLVAFGVAPAVMMYKWALV
                     PFGRWGWAATFLFVACGALRLARFNIQTGMPGSKSFKGMPIPGAATVMASIVIFYYDF
                     WKGVPEQNVFFLVITILLSLLMVSTIRYHGLKEIDFREKKPFWFLIVFVIILFVLFVH
                     PSTAIFIFAMAYLVWGIIENGYLLVKRRRAKSLERQALFEGSSKQGAERSDSTLQEVK
                     DEGHQDI"
     gene            15724..>16339
                     /locus_tag="VMU21_09975"
     CDS             15724..>16339
                     /locus_tag="VMU21_09975"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546793.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HUO77889.1"
                     /translation="MRVIKIFDTTLRDGEQSPGASMNVEEKIQVAKQLARLGVDVVEA
                     GFPIASPGDFEAVKRIASEVKRITVAGLARAREEDIKRAWEAVKDAPQKRIHTFHSTS
                     DIHLKYQFRVSRDEALKRSADMVRLARSLVEDVEFSPMDATRTETNYLLEVLDAVIEA
                     GASTVNIPDTVGYTTPMEFGTMIKAIKERIGERAVISVHCHNDLG"
BASE COUNT         4131 a         4004 c         3796 g         4408 t
ORIGIN      
        1 gttgtgttgt caggcctgac gatgttaagc acgaaggtct gctttacatt cgagatggcc
       61 ttcttggtat ctgcatgatc cgcagttttc gcaggcttgt gctccttcat agggcagtga
      121 gaacccttac atgttacaaa ggctgtcttt gctgcccatc cttgggggca ggtattctgc
      181 atgccgattg cgacggcaag tacagggatt aaaacatgcg gccacataga cctattgtaa
      241 ccttttttgt gaataaaata tgaaggaaga tattgaaata cacatcaaag accatgctca
      301 gaatattaga agagaaataa ccgcaacggt caataccaca tagagaatat cagacttctt
      361 aaccagcgcg gcacaggccg cacagaccaa taagacttcc tttgcgcgaa atgcggcatt
      421 aatatagcaa agcacccgcc acgccagagc cgaggataac gaatgtcggt tctactttcg
      481 taaatacgag tatagcaaaa ccgcccgttg ctatgaggcc ggtaggaaca tcgacaatgg
      541 acgccctgcc cagggtgatc gcgattgcgg caagaagccc gatcacggcg gggcgaatcc
      601 cttgaaagct tgctttgacg atccacgagt ttttaaactt caaataatag tgactggcaa
      661 tcgtcacaag gatgattgaa ggcagatacg agaagataac cgcgccgaga gcgccccaaa
      721 acccgaatat cttgtaaccg atgaacacat tcatgatcgt cagcggtccg ggagttacct
      781 gtccgattgc gatgctttcg acaaattcct tgttgctgag ccagtgataa tgatcgacga
      841 cctcccgctg aaggagcggc agcatcacat agccaccgcc aatggtaaac aagttgatgt
      901 aaaagattgt ccagaaaaaa gtaaagattt ccatttcact gttctcctct ggacatgagc
      961 agaccgacaa tgcctgcggc gacgattagc ccgatcggat tgagtttgaa tacagcgaga
     1021 cacaggaagg cgacgatggc aatcaatatg cctttgtact ccttcagaag ttttccgctc
     1081 atggaataaa caaccgaggc aatgagggcg acgactgcag cgccaacgcc tttcagcacg
     1141 gccttgagta gtgcaatttc cttgtacctg atatacagat aagagaggat caggacgatg
     1201 acaaaagacg gcagaacctg gccggcaaca gtggcaagcg ccccttttaa cctctttaat
     1261 cggtaaccga tgaatatgcc ggtattgatg atgggtgcgc cgggagtcaa ctgacccatg
     1321 acgacgctct cggcgaattc ttcctgactg agccacttcc tgttctgcac gagttcccgt
     1381 tcaaaaaagg caagcatcga atagacaccg ccgaatgcga atgtgccgat tttgagaaat
     1441 acttttaaga tattttttac agatgcgtcc gcgaccgtca tatgattcgc cgtatcaatt
     1501 tttgatccac tctcttctga gtgcattgtc caggatgacc cccacaagaa aagccacccc
     1561 catattccac attgcaacag cggccacgac aatcatcaca taaaactcag acttcttgcc
     1621 tccgatatcc ctcacgacta tcgccagttc cgaacccgcg aagaacagaa ttactcccag
     1681 tatggcattc gggaatatct tgaagataat ggataccgat tcgctgaaaa acagggcaat
     1741 aagtatgagt atgctaccca atataaccag cgccccgccg gtctttgcgc cgaagcgaac
     1801 atgtcctgcc attccgcctg cgccgtgaca catcggaatg ccgccgaaga ggggcgcaac
     1861 caggttcatg atgccttgtg aaatggccat tttcttctcc gtcaccggcc tgtcagggaa
     1921 aagctcgttg ttctcggcag tgattgcaat caccgcgttg cccagggtta acggtatttg
     1981 gggaattgtg aaaagtaatg ttcccacaac gagatcgttc catgtaatca tggggatact
     2041 gaaaaccggc agattgaacc cgatatgaat tttggccaat tccgacatta tttgagggtt
     2101 cataataacg gcggacacaa ccccgatgat caggagcatg aacatagccg gtattttggg
     2161 gtttgtgaga agaaggtacg tgacgaccaa ggcgatcacc gcgaggacgg gcgctgtctt
     2221 catcctgttg actccatcga ccatgaaggt gagccccagc cccagcatga tgccacgcac
     2281 aaccggcttg gttgcaagtt tggctatcgg tctgaggacc cccgtaatac cggcaagaag
     2341 ccagaaaagc cctgtggtga ggcctgatcc ataaagggct gcggggctta tacccccggc
     2401 gatggctgca gccccaatgg ccttcatggg ttggatcgga agaggcgtcc tgtaatagag
     2461 cccggacgct attagagtga tgccaaacat gaagagaagc cctaggggcg gcatcttaac
     2521 gatagtgata taggcaacga caaaaggaat cagagtgccg acatccccga acgcccccgc
     2581 ccattccatc cggttgtaaa gattttttgt ctttgcaacc gcaatacccg catcagcttg
     2641 cgcctcggta accggatcct tagcaacccc accgctcttt tccaccatga aatacctcct
     2701 ttcaaatatg tcattttggg ctaactctat ttcccagcgt ttcgagttgg cccgggtcgc
     2761 cgttctccgt ggacaccgag accgcctttg tcaaactcgc ctcggattac ttcacagaat
     2821 tgtctcttct gctcggggga aaggagttcc ttctcctgga ggatatggcc gatcacttct
     2881 cgctcaagtt ctgcctgaag cgccgagatc tcgccgctct tttccccgat cgtcttcata
     2941 tccggctctt ccgcaaaaag gaggtcgttc aactgaagcc tgttgagtct aagcgactgc
     3001 cggattcctt ccactttcgg caggaagtcc cttcgtatct cctcgacctg tcgcttctgc
     3061 tctttggtca gcgtgataag gtcaagatat cctctaatat gtttttggcg tattcttgag
     3121 accaggctga ctatgagttg agcaataacg attacagaaa gcaggataag aaaaatatct
     3181 ttcaattgcc gcctccttgt ataacagtaa ggtaaacatc ggcgaatgat ccgggaggtg
     3241 agtcgtcaaa agtgcgcaga taaccatatg aagagaggct tctctcgcca tgaataccgt
     3301 atccgttatg ggcaaagaca aaggcgactg cattgcggag aaaggagagc gcgggattga
     3361 gaaaaaactt gttccacaac gaacgccgac gtataacagc ggccttgtca acggttctct
     3421 gtacgaaggc cagcgaaggc gtttcttcag ggatagtccc cattaatagg ccgaactgag
     3481 caagagtttc cgcttcacgg gcgcaggagg gacacgtctt aagatgctcc tccatcaaaa
     3541 agcgctcttc ggaggaaagt tcgttatcga tatatgccga taatttttct ttacagtagt
     3601 cgcagttcat tctatatgtt taaacacgga tatcagggaa aaccttcggt agggcaaaat
     3661 tattttttaa gataggggaa aagtgacttt ttcaggcttt ggcgcgcccg ctggatcagg
     3721 gattcgattg cggaaattga gaggcccatt atcttgctga tctcattgta ggagaagtcg
     3781 ttgtattgcc ttaaaagtat tgcggtcctc tgattttccg ggaggcttcg gattgcctta
     3841 caaacagcct tactcagctc cgcattttcg gccgcttttt ggggagagaa agcctcagga
     3901 gcaataatag gatattcttg ttcgtgcaag tctgataaat caacaaattc gcaactgctc
     3961 ttattatccc ttatttcatt taagcacagg ttcgtagcta tggtaaaaag ccaggttttg
     4021 aattttgcct gcggcttata gtcttttgcg gctttgaata ctcgcagaaa tacttcctgg
     4081 gcgatatcct ccgccgaatc cctatctccc gtaaaccgtg ctatgaagct tatgagtggt
     4141 ttatggtgga gttcaacaag agttttgaat gctgatagat tcccctcagc aacctctacc
     4201 attattttag tttctggctc ttccatcgcg gcttaattct gccttaaacg gcgtaatgaa
     4261 tcaagtgctt tgacagtgct ttgatagcaa taaaaaaggg cgggattgat tacttcccgc
     4321 ccttttcact acaacccgat attacttttt tccaaacggc actgttggga tgttcgtacc
     4381 gtgggatgaa tttgactgtt cttatttcca attattcagt acttggaaac tccttgccct
     4441 ccttcatccc aaagtacatt gccgatttcc cgactggtat cttcgacaca acttccatct
     4501 ttgcaacgtc gatcttattc ataatgttgt cttcagcgtt gatcatatag aagtatttcc
     4561 cgtcaggagt gaagtacgag ccatgcgcct gttttttacc attgccgacc aatatgtttt
     4621 ttaccacttc ctgttttgca gtatctatga ccgcaacgat attgtcattg tgatggatga
     4681 cgaagatata cttgccgtct ctcgacatgc tcgggtggcc tgcacccatg ccgactttca
     4741 gggttttgac cacttccatt ttcgcagtat ctatgacggt tacagtcctg ccgccctcgt
     4801 tgcacacgaa gaaatagttt ccatctttag tgaatactcc atgatgtccg aggcccttct
     4861 ctcctggttc gagagggata gggatatccg aaatgatctt gtctgtatca ctgttgataa
     4921 gagtcaccga tgtgcctgtc ttcgtcgtct cattcaccgc gtagagcact tttccatccg
     4981 gcgaaggatg gatgtaatgg tttgagccgg gcaaggcgat tgcagccttt atctcaaaat
     5041 cagcaacatc gatcttgacg atgttgtagt tgaaatcatc ggaggcatag agaaatctgc
     5101 cgtcctttga ccaatatgag tgcctcatca tagcaccctt gaaggcacct tcaggctcct
     5161 ttccgactgg gatggtcttc attatattgt ccgtccctgt gtcaataagc gaaactttga
     5221 gggcgtcttt gtaggcaaca gcaagggttt ttccgtccgg tgaaagggca agctgaaaag
     5281 atgttgcagg aagcgcaatt gtcttgatga cctcaagcct gtttgcatct attaccgtga
     5341 gatcttttgt ccctctgttg ttcacatagc actttccgtc ctttgtaaac gcaagagcat
     5401 gaggtgtttt ccctgtcgga agagtcttga tcaccttctc cgcgataagg tccacaacaa
     5461 ccacgttgtt gtctccttca ttaggcacat aggctagtct ttcttcagcg gaagatgtga
     5521 aagcgcctgc gactaaactc aggcaaagga taactaacag agcagttttc ctcatctcgt
     5581 ttcctccttt gtggtatgac tgcccgtaag tataagactt ttctctctaa taaatcactg
     5641 ctgagcatgt cttttccagg caggttcact gcttgaaaat gccaacaaag acaatatcta
     5701 caacagggcg aatcaatatc ttagcgttgc tttcttcctt tttggttcaa gaagtaaatc
     5761 aagaccgtca atgattgaag taaccatgac atcggcgaat tttagtgcat ctgaagcgca
     5821 gccttcaact agacagacag ctatgcctat ttaagctgtc ctgagcatca gccggtcatt
     5881 gtttccgttg ccataggcaa taagattttc agcgtcgaga ttcataacat agtgctcctt
     5941 gtggagatgc agggcaatat gatactcttg ttcgtaagag tatgagttat tagcttcgga
     6001 cgcagaagcg gataacggcg aactccatcg cccttaaaac agccgcttgt aacaagctat
     6061 cagaatatga gaggatagtt aaaataaaaa aactcatgcc tttagtgaac gggcaggagt
     6121 ttatagaaca gtctgcattt tcgatgtcca atccctaccc tacaatttcc caattagact
     6181 caaatgtgtc gtcatacaca gtgtaaacga agcccatcag ccttccaact ccaggcagta
     6241 cgcccggctt aatacgccag taagacagat aaattcctgg aactaggcgt ggctgatgtg
     6301 atattggatc gtcttccgga gcattctgcc aaacttcgcg tttttccatt acccgccata
     6361 aagtcccata ctttttgctc tttactgttt gtccaacccg gggtaacttc ttgtgttgtt
     6421 ccacttcttc atgttctttc atcgggatac tcctcctcac gtattcatgc agttgttttc
     6481 aatctaatat aaatatcatt tgatgtcaat agaataacaa gtgatcgaat tgactcacaa
     6541 aaaatgttac ccaagcaaga gactgggaag aacgctgact cataatagat gttaccatat
     6601 ctgtatttat cggggcgaca tttaaagacg aacgccttag cacacttacc tcccatcatc
     6661 gttgaaaata cggtctcaat cctgatgcca attcctcagg aagattgatc atcagctccg
     6721 aatggagcag tttctgcagg aaataatcat cgttccggta gttggggtag agattctgcc
     6781 tgctgattat cttgaggaga cagcgaaagc ccttttgaga agaaccatct ctgctcgaaa
     6841 agatggaata attgaagctc aggtggccga gactctcata aaaggagagt accctggaaa
     6901 tacccttagc cagggtcgcc aaatcgcttt cggaaaggag cgcaaaatca ttttttcctt
     6961 catggatagc aacaacttca ttattgccca tgggagaaaa ggcggtcatc caatgccatc
     7021 caccactttg agccacatat ctttccccct ttcgcttctc ttcatctatc aaatccagat
     7081 agaattcaga gccatgctct tggtagtaag aatggcttgc ccggatgagt ctatcatgat
     7141 atgaatatgc ggtgggggta atcagcatct gcagatgagg gtgcacaagg gtggctccag
     7201 cagggaaaag gtaattggcg ttcacagcca caaacgacgc tgaaggatca ttgttataca
     7261 caaggttgac aaatgtctgt gccgccttca ggccgttctc gattatgtcg ggcctgaatt
     7321 cggaaagttt cagaaaatga gccttggaaa ggcgaataac ggaatggtac tttcctatag
     7381 gaaaaaggtt tggaaacaga acggcctcgc ctgtttttat tcgtccttcg gggacaagat
     7441 tcggcggata tctgggagtg cttctttcga tcatgtcgcc gcaaaaaata caatttttcg
     7501 cactgtcatc tatcaatttt tctacaaggc caatgtcaca gtccccaaaa aagaatttta
     7561 ctttatcttt aaggcgaggg ttgtagacac ttatatcgcc taaaagcggg tctttgcgga
     7621 cctccacggt atgcacttct tcagaaaagt ccctaaaagg attcataagt gttaaatctg
     7681 tatcagaggt ttcaaatctt attttggaca tactgcctcc tatggttttg caaatcgcca
     7741 agttgttaag cgagtaagac gcttcttctc accagtgcag atctcctctg aagactgccc
     7801 tgtcgcccaa ctcttcctca atcctaagca gctgattata ttttgccagc ctctccgagc
     7861 gggcaggcgc tccggtcttt agttgtccgg catttgtggc gacagccaca tccgcgagga
     7921 acgaatcttc agtctcccct gaacggtgcg aaaacacgca ggtataaccg gctttctctg
     7981 ccagtttgac cgtctccagt gtctccgaga gagtccctat ctggttcagc ttgatgagta
     8041 cggaattagc gataccctgc ctgatccctt cggcaactat tttggagttt gtcacaaaaa
     8101 tatcgtcacc ggtaagctga attttgttgc ctaattgttt cgtgagtacc tcccagcctt
     8161 tccaatcgtc ctcggcaagg ccgtcttcga tggaaatgat aggatacttt gagatcagtc
     8221 tctcgtaata agttaccatc tgctctgatg acaggaggct gttatcggcc ttgagaatat
     8281 atttcccttc ctgatagaac tcgcttgccg cagggtcaag cactatcgct atatcctgac
     8341 cgggcttata tccggccttt tcgatggcag taataatgac ctgtatcgcc tcttcattgg
     8401 aggagagatt aggcgcaaag cccccctcgt cgccgacagc tgtgctcaac ccttttgatt
     8461 tcaaaacagc ttttagattg tgatatgtct cagcgcccat ccggagcgct tctctgaaag
     8521 atttagcccc tatgggtgca atcatgaact cctgaatatc cacattgttg tctgcatgtt
     8581 ttccgccatt gaggatatta aagaaaggga tcggcaggcg gcaggctttg ttgccgccga
     8641 tatactgaaa cacagatttt cccatatcct gagctgccgc tttcgcaact gcaagggaaa
     8701 cgcccaatat ggcattggcg cccagcctgc ccttattttc tgtgccgtca agtttaatca
     8761 tgagattgtc tatgaccttt tgctccgaag gactctttcc gatcagttca ggggcaatta
     8821 tcgtattcac attgctgaca gccttctgga cccccttacc taaaaagcgt cccttgtcgc
     8881 cgtctctcag ctcaaccgcc tccttgctcc ccgttgaagc gcccgaagga acggcggcac
     8941 ggcccaatat tccattatcc agaactacgt caacttcaac tgtaggatta ccccttgagt
     9001 ccagtatttc ccttgcaaat atctttctga tctttgacat agccttcctc cttttcaata
     9061 actgtattgg cctgccgggt tccacttctc gaaaaagcgg tccaccttcc caaagccaac
     9121 aaaacttctg tctgttcttc catcacttcc tgtaagctgt ctattatttt tcatgcaata
     9181 tcctcaatct tagaatcaat catgatgagc tttaaattca acctgttgtc tctcagatat
     9241 tccggccttc gtgcggtttc aaatattctc gagatggctc tgggattgcc ttttgaaata
     9301 tggaaaacct catcaaacat tcttcgcttt gttgagtaat cgatattgag attatcgcag
     9361 agaatttcca tcaagacaag ggcatccttc tttctcagat gatcaagcct caacttcggg
     9421 atcaaataaa ggcaataatc aaggcgccct ctgaaggaat agtcactcaa ctcccagctc
     9481 tgtttgcttg aggtgatcac cggtgccttc tcgtaaagtg tggtcaggaa tccgttgatc
     9541 ttcggtgtta catattcaag atggtcgaga atcacacaaa aatccgccaa agagatgtag
     9601 gagaagacaa catttctgag tgcgcggata ctcagcttct tgatgtctga gccgcattgt
     9661 atttctttaa cacgcttgaa cttgtcaacg ctttcaagat aactgaaatg tgaaagaagg
     9721 aagagggcca tttgaagcag aatacttctc aatgtcctgc tgctcctgca atagatcggg
     9781 ataggggtac cccgagtttt caggaaatca tacacttgat cgagaagagc gctcttgccg
     9841 gtacccccct ccccatagat atgaaggctc tttctctctg cgatgtgttc caaaataacg
     9901 gcaaactccc tgtcacgcgc gattaaggat atgtcaggcg aaccacggtg ctttcgggaa
     9961 cgattactcc gcctatcaga acctgctgat aacacccagg gcctctctga gtttcatgag
    10021 cctgtcctta atggcgtcga gccgcgtcag aatatccgag acctcgttct tcctttcatt
    10081 cgcccccttc tcgcttttgg accgctgaga cattcctttc ttgatggcag ctccgatgcc
    10141 ttcgatagtc gcctcgatcc tctggagcat ctcccatctg aaatcgagtt tgcttttggt
    10201 gagccttttt tcgaagtcgg aaccgatccg gcacaattgt atagttatca cacgatggag
    10261 atattccttc attttcctga tgatgatctt ttcgccgata aacttaggaa gtgagagggt
    10321 gagtgacgat gtaattatct caaggcccac cggctgctcc gtgaacttat agtaaagctt
    10381 ggatttcgtt gaccagagcg cctctgcctt gaccgcatcg tagggaactg caaagagttc
    10441 ggatgagaac ttgagcagag aatccacagt ttcatcgatc tttatgataa agcgtttgca
    10501 cgccgtctcg aacgtctttg caagtttctc atcctctgtg cctcgccagg cattgaccgc
    10561 ttgcctgacc tcagagatga tatgttcttc aaggtgctta tggagttctt tgagagacaa
    10621 tccagtgttt tcgcgatagt attcgtcaag attcgcctcg gcctttaaac gcagttcttg
    10681 tccataagca ttcaggtctt catcgagaac gttcttcata atctttttta tctcaccgtc
    10741 caggagaagg tcgaagtcac ttttttccgt caggacctcc ttcttcctct cctcaaatgt
    10801 ctgtatcttc tccttcagtt cctcaagggg cgtggtgagg gattttatct caagttcaag
    10861 ctcgaaccgt gcttgagaga gcagccgaag aaggttgttt gtgacagaga gaatcagtat
    10921 ctttcccttt tcttccatga ggaaagtgtt tagtacttcc gagaactgcg gaagaaggct
    10981 cttttccacg atatcttcag ctcctgagag cttcccttca agggccagtt tcgccgagac
    11041 aggaaatatc cgtatgtcag ttaccatcgc ctctttgagc acatttttcg agaattccat
    11101 ggattcccgg agatcagcct cacagaagta gtcagcttta tttagaagaa agaatatcct
    11161 cttggaatac tcccgcacat ctttaaggaa ttccagttca gccttgctca gaggctggtc
    11221 tacagaaaga agaaaaagcg ctgcgtctga tttaggcaga tatcgatagg caacgtccgt
    11281 attatgctcg taaactgagc cgacgccagg cgtgtctatt atctgaaccc cgcccctgag
    11341 atatggagag ggatagttta tgataacttc tgagacatcc ttctcgtttt tcgggttacc
    11401 cctttccgta acgtattctg gaaggctctc agtcttgatc tcggcaaccc ttccctcatt
    11461 gaaatgcacc acgattctca gggcgtcgcc gtatttcata atggtgacaa cggaggtgag
    11521 agggacgacg gccacgggaa gaatgtcttc tccgagaagt gcattgatca ggctcgtctt
    11581 gcccctcttg aactggccta ccacgacgag attaaaagta tttgtctcga tcttttcccg
    11641 cagctcctca cacgggcagc ctcgcattgc ccctatgccg agtatgtcat ctatgcaccg
    11701 aatcaactcc accttaaggc gttggtagtc ggacattgtt gcgggttcaa gcttttccat
    11761 tgtgggccaa cagcctcctt tcacgctaat aaaaaactcc taccctctgc ttccaagagt
    11821 aggagttatc agtccggttc ggacggttta tccctttaga gattgcgctt ctctaaagga
    11881 gttaaaggcg aactccatcg cctgttagat agtttcagga ttcacatgct tgtgtgtgta
    11941 cttaatgaca gtcacgtctg aaaggactac aatcccttcc cccaccatat cgtcaagaat
    12001 cggtaccact ttgttgatct tatcttcagt atcaataacc gtaatcagga tcggcagttc
    12061 gccgtgcaca gtcattgttt tcttcttgtg gtatcgcctg tgcgggccgt atccggcaat
    12121 ggctttgtat actgtagccc cggcgatatc gctcataata agtcgtttaa cgattgcttc
    12181 gtacaacggt tccccttccc atttatcgtc ttcgcttata atgatgcgaa gcatctttgc
    12241 cttgccttcg agtttcatat gttctccttg ttctttttgt ttctcttccg ccttcgggca
    12301 gcatttgatg acgttcgtgt cggcaacttc cacaagacct ttctccacga catgatatac
    12361 gtcaggcagg accctgttga tcatgtcctc agaatcgaca acttcaatcc ttaccggcat
    12421 gcttgttgaa agctcaagca tcttggcggt atggaatatc ccatcagtgc cgtatcctgc
    12481 gatgcccctg aagacacttg cgcctccgat cttcttcttg aagaagatgt ccatcagcac
    12541 ttcatagact ggcttaccat ggaacttatc catctcgtca acataaatag tcagtttttt
    12601 tgcagggcct ttggtcacca tatatttatc tcctatatgc tccttgcaat cacgtctccg
    12661 agtttcaacg ccatgaaacc tacgagtgtg cttaaaacaa tgttcatcgc tgcgtagacc
    12721 agttccccgt cggcaacaag cctaccggtt tcatactgga acgtcgaaaa ggtagtgtaa
    12781 ccccccaagc ctccgacaac gaggagaagc ctccactcag ggttttccat gaagcgctct
    12841 gccatcaggg tcatcagtaa gccgataaga aagcttccgg tgacgttaat cacaaaggtg
    12901 ccgagaggaa agctccgtcc ccaccgttgg ccgatccagg tagcaagcac gaagcgtgca
    12961 acagaaccga tacctccgcc ggcgaataca acaagatagt tcatcattaa gagagcatcc
    13021 tttaatacct atttaacaat tgtaactgtg cagggcgcat aggttgcaat cctctttgaa
    13081 accgaaccga ggagccagct ttctattttc gattttcctg tttgtccgac aacaaccata
    13141 tcacaattct tgtcttttgc gtatttcacg atctgatcgg caggatggcc cacaacaact
    13201 tcagtcttta tctccatatt tttttctttt gctttttctt tcagatcttt gaacaaccct
    13261 tcgtaatgct gtgttgcgct gtcgatgatt gcatccacct cgacaatatc tgccggctca
    13321 gggggttgtg caacggaaat aaccatcacc tcgggggctg cgccaggaca tagttttgtc
    13381 atttcaaggg caaagtcaaa ggccttgtat gattgctgtg acccgtcaaa ggcaacaagt
    13441 attttcttca gcataagatc ctcctttttt ttattcttcg ttgacagcat ttgtttctaa
    13501 tgttgctttc ttctctcttt ttggcagcag atagtgcgga ataaaaaagg cattggcaat
    13561 aagtgtgggc accacggcgc ttccaatgac aacgaagaca agaatggaat actgctgctg
    13621 atttatgata ttgtgagtga gcccgaagag tgcagagata gatccaaagg tgagtcccgt
    13681 tgacatgaga agagtggtat aaataccttc tcgctgcgca taacgaaagg tcttagttgc
    13741 gggatagacg ccgatgatct tggttatcat cttgcctaag aacaatacaa gaattgcaac
    13801 aggcgctgcg ataagtgccg ggacagaaac ataggagccc gccctgatga aatagaaggg
    13861 tgtaagcagt ccgaaggtca gcgtccgaag gcgcctgatg agcacatggt ccttgcccac
    13921 ggtcccggcg agtaccatgc caatgatata cgcgggtagc actgcttcgc tgtccgacca
    13981 cactgcgagc gatcccaagc cgaaaagaaa cagcaggagg tatttggctt cgagctctga
    14041 cggccggtcc ccgtattttt tgaagaaccg aggtgtaagg taagggagga tgaagaatgc
    14101 tgcagcgctg accccaacaa agatcagggt tttcagggtg aaaggggcga agatgagacc
    14161 cagggcgagg acggtcccga gatcggtgac gaagcaggca gcgagcactg ccttgccgaa
    14221 gtcggtcttg ttgagcccca gttcgagcat caccgcgtaa accacagcaa ccgaggtcgt
    14281 cgaaagggcc acgccggcaa gccagcttgc ctgcacggtc caatgaagga agtagtatgc
    14341 ggccgctgtg cagccaagga aaggcgcaag gaaggagata aaaccaatgg ctgaggcttc
    14401 tttcaatttt gccttaaata cgactgggtc aagctcagca cctgccagaa aggtgagaac
    14461 aatggctcct gtgccagata agaatttgat ccatggcaca tcagagccca gagtcgttcc
    14521 gatgaatgcg ccgatgatca attgggccac tgtgccgacg acgatttccg aaagggctgt
    14581 tgcaatgcgg aaccagatgg aaagtaaagt ggcaattaag gcaaggccta tccagagtgc
    14641 cgccatcccc catacttcat gcataaccca tctcctttcc gggaataaaa aactcccacc
    14701 ctcttgagta tatactgtga gagtaggagt tatcagtccc gataaatcgg gacggttaat
    14761 ggcgaactcc atcgcctgct cacgtaagat aacagagagc cctctgtatg tcaattacct
    14821 acgaggagga aatcacaggg gatagatacg atttctcacc cggatggagg tttttctatt
    14881 ataatatcct atatatggtt atcaaacata aaagaggggg cgttgagaaa cagcccaaga
    14941 agggggttta tctcttgccc aataccctca cgttgtgtgg aatgttttgc ggtttttttg
    15001 ccattctctc cgcaataaac ggcaatttcc tctatgcggc atgggccatc gtccttgcca
    15061 atgtattcga tggtcttgat ggctggatcg cgcggttaac caacaccacg acacggttcg
    15121 gaatcgagct ggattccctt tccgatctcg tggcatttgg tgttgcccct gcagtcatga
    15181 tgtataaatg ggcacttgtg ccctttggga ggtggggctg ggctgctacc tttctttttg
    15241 tggcgtgcgg tgcgctgagg cttgccagat tcaatataca gacaggtatg ccgggatcaa
    15301 agtcgttcaa aggaatgccc ataccggggg cagccacggt gatggcatcc attgtgattt
    15361 tttattatga tttctggaaa ggtgttcctg aacagaatgt gttcttcctc gttatcacta
    15421 ttttactttc tctcctcatg gtaagcacca tccgctatca tggcctgaaa gagatcgatt
    15481 tcagggagaa aaagcccttc tggtttctca ttgtgttcgt gataattctc tttgtgcttt
    15541 ttgtgcatcc ttcgaccgca atatttattt ttgctatggc atacctcgtt tggggtataa
    15601 ttgaaaacgg ttaccttctt gtaaaaagga ggagggcgaa atcactggag cgacaggctt
    15661 tgtttgaggg ctcgtcaaaa cagggtgctg agaggagcga ttcaacgctc caagaggtga
    15721 aggatgaggg tcatcaagat atttgatacg acgcttcgtg acggggaaca gtccccgggg
    15781 gcgtccatga atgttgagga gaagatccag gtggcaaagc agcttgcccg tctgggagtt
    15841 gatgtggtcg aggccggatt ccccattgca tctccaggag attttgaggc tgtgaagaga
    15901 attgcatccg aagtcaaaag gatcactgtg gccgggcttg ccagggccag ggaagaagac
    15961 ataaagaggg cctgggaggc ggtgaaggac gctccccaaa aaagaataca taccttccac
    16021 tccacatcgg acatacacct gaaataccag ttcagagtga gccgtgacga ggcgttaaag
    16081 agatctgcgg atatggtgag attggcgagg agccttgtgg aagatgtaga gttctccccc
    16141 atggatgcga cgaggacaga gacgaattac cttctggagg ttttggatgc ggtgatagag
    16201 gcaggggcct ccacagtgaa tatccctgac accgtgggct ataccacccc gatggaattc
    16261 ggaaccatga taaaagccat taaggaaagg attggggagc gggcggtaat ctcggtacat
    16321 tgccataatg atttaggac
//
LOCUS       DATKOU010000212         4611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3764297, whole genome shotgun sequence.
ACCESSION   DATKOU010000212 DATKOU010000000
VERSION     DATKOU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3764297"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VMU21_09980"
     CDS             <1..692
                     /locus_tag="VMU21_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77890.1"
                     /translation="ETIGADAFQKEEEEAITYLESVKTKALQEGLSCQSMLLYGDEPY
                     QLIVDTAAEKMVDMIIIGRHGRTGLMKVLMGSVATKVIGHSRCKVLVVPKAARIEYRN
                     ILLASDGSEHSIAAASEAIGIAKRCGSNIIALSVAHSEAELKEAKTNVSRVIEMAQKD
                     GIPAEALTPIGKPYDVIIETAGGRAVDLIVMGAYGKTGLRKVFMGSTTEKVIGHTACA
                     VLVVKAELPQS"
     gene            727..1074
                     /locus_tag="VMU21_09985"
     CDS             727..1074
                     /locus_tag="VMU21_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HUO77891.1"
                     /translation="MEPSSARYIEFQGKTIEVDEEGFLKSDKDWSEELANYLSKEDGI
                     ELSKDHWEIINFVRWYYITYQNSPHPRFIIKKLNKRYGSHKYTVKYLFELFRKYPVKR
                     ACKYGGIPFTEGT"
     gene            complement(1428..2564)
                     /locus_tag="VMU21_09990"
     CDS             complement(1428..2564)
                     /locus_tag="VMU21_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUO77892.1"
                     /translation="MNEERHKGGTSLTDLRKLRHQMPRWVLALLVFGVAAVVASRITH
                     LLMMLIAAAIIAYIFSSVICALERLGIKRSVAVILLFVLVTVFVIAADLIFIPDLKQE
                     IMNSYAKFPEFSRQTQDALLFTAEKYPFIESSITKLVDDVFGPSGFLERTLSASEILM
                     QATPFIMALILVPFFVFFLLKDWPGVMKRVMDWIPPSYVETTISVIAEINILVGKYLR
                     GLAGDCFVVGVLASVGLWLIGINYPIMLGILSGVANIVPYLGPIIGCTISSLVALMQY
                     QSFDPVLNVVVLYLAVKLLDDLVIQPLMIGKSVHLHPMLLVITLIVGEKLFGIMGMIL
                     GVPFVTAAQKTAAILLEHRRETHKRVCAERLILHRLGKSPVRPI"
     gene            complement(2584..3072)
                     /locus_tag="VMU21_09995"
     CDS             complement(2584..3072)
                     /locus_tag="VMU21_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUO77893.1"
                     /translation="MVLDNKIVRRYFTWALDTTRESHLRTLSDVPLFTGMGKKLLRKL
                     LIDLIEKKYTAGDIIFHEGESGKAVYVVIEGSVKIIKKSNSESKTLAVLGPGSHFGEL
                     SLIGQCSRFATAVAEKDSLLLIMYKSYFDDLIRGNSAISSRILLNLAEILSKYIQRDQ
                     PE"
     gene            complement(3207..4109)
                     /locus_tag="VMU21_10000"
     CDS             complement(3207..4109)
                     /locus_tag="VMU21_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUO77894.1"
                     /translation="MARPNKLIVILGKLLVSSGLLYFVISKTGADKVISTLKGINPLA
                     FFLAVFIYIFAIYLSSVRWRLLLHEEFELRKLFSLYFIGSFFNHLLPGIIGGDAVKAY
                     YLYKDTGKGAPAIASVFMDRYIGFTALMCVGLTAFPFGLRYFRGSYIEWILPLMVFLF
                     ILGSFVIFGLKVGRRIRLLSGFYDYFSLYKGKKTVILKTFFLSLIVQVVIIFAVYVLS
                     LGLKVDVPLLPLFIFVPIIATISSVPISISGLGVREASFVLLFGSLGIKPAQATAVSF
                     AWFLSVATGSLPGLIEYLRYKKGM"
     gene            complement(4093..>4611)
                     /locus_tag="VMU21_10005"
     CDS             complement(4093..>4611)
                     /locus_tag="VMU21_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO77895.1"
                     /translation="VTGVKLHDYGCSLKAYRRDVIKNLKLYGEMHRFIPAVASWYGVR
                     VAEVETMHHPRLRGKSKYGITRTIKVVLDLITVKFLQSFSTKPLQFFGSIGMAGGAIG
                     FLILFYLSIDKVIFGNPIGGRPLLLLGALLLIVGVEFIGMGLLGEMIVRVYHETQRKP
                     IYVIKKVLGPSK"
BASE COUNT         1364 a         1052 c         1123 g         1072 t
ORIGIN      
        1 atgagaccat tggtgcagat gcttttcaga aagaagagga agaagcaata acatatctcg
       61 aatctgtcaa gacaaaggca ttgcaagagg gattatcctg ccagagcatg cttctttatg
      121 gggatgagcc ttatcaactc atagtggata cagcagctga aaaaatggtc gacatgataa
      181 tcataggaag acatggccga acaggactga tgaaggtatt gatgggtagt gtggctacaa
      241 aagtcattgg tcattcacgc tgcaaggttt tagttgttcc aaaggcagca aggattgagt
      301 acagaaacat attacttgct tcagacggtt cagagcacag catagcagca gcctcagagg
      361 cgattggaat tgcgaaacgc tgtggaagca atattatcgc actttctgta gcacactcgg
      421 aagccgagct gaaagaagca aaaaccaatg tcagcagagt cattgaaatg gcccaaaagg
      481 atggcatacc cgcagaagca ttgacaccta tcggcaagcc ttacgatgtt atcattgaga
      541 cagcaggtgg cagggctgtt gacttaatag ttatgggagc gtatgggaaa acgggattaa
      601 gaaaagtctt tatgggaagc acgacagaaa aggttatcgg ccatacagcc tgcgctgttt
      661 tggttgtcaa ggcagagcta ccccagagtt aaagagttat aattatagac tgtgggaagg
      721 ttaaggatgg agccatcgtc ggcgcggtat atcgaatttc aaggcaaaac aatcgaggtc
      781 gatgaagagg gttttctgaa atctgacaaa gattggtccg aggaattagc gaattacttg
      841 agcaaggaag atgggattga actttcaaaa gatcactggg agattatcaa tttcgtacgg
      901 tggtattaca taacctacca gaacagccct catccgagat ttattatcaa gaaactcaac
      961 aagaggtatg ggtcccacaa atacaccgtc aaatacttat ttgagttgtt ccggaagtat
     1021 cccgtaaaga gagcatgcaa atatggcggc attcctttca ccgaaggcac ctgatacgca
     1081 tcagaattag actggatgtc taatcaatga tatagggaag ggtagaatga agcgcccccg
     1141 gtattttgag ggtatgaggc agccatgtgt tattcctcca gagcacatta attgctgata
     1201 catcactttg gtatattcgg cgcttgcagc tccggctgaa tttcacgcgt tactccacat
     1261 ttctttcgga aacccgcaat agtttcgatt cgcaataact atcaggtcgg tgtactgcgc
     1321 aaaaatacca tgcacattac agggtgaaag acgtatggag caataccaac gctttaggac
     1381 agaatctttc cccgtgtcat ccaaaaaaag gaattgttca agaaacgcta aatcggcctg
     1441 acaggggatt tgccaaggcg atgcaatata agcctctctg cgcacaccct tttgtgtgtc
     1501 tcccggcggt gttccagcag gatggcggca gttttttgtg cagcggtcac gaaggggacc
     1561 ccaagtatca tacccattat gccgaagagt ttctcaccca cgattaatgt gatgacgagg
     1621 agcatgggat ggaggtgcac gctcttgccg atcatcagcg gctgtatcac gaggtcgtca
     1681 aggagcttta cggcgaggta aaggacaacg acattcaata cgggatcaaa gctctggtac
     1741 tgcatcaggg cgacaaggga ggaaatcgtg catccgataa tggggccaag atacggtacg
     1801 atatttgcca caccactcaa aatacccagc atgataggat agttgattcc aatgagccag
     1861 agtcccactg aagccagtac ccctacaaca aaacagtcac cggcaagccc ccggagatac
     1921 tttcccacga ggatatttat ctctgcaatg accgagatag tcgtctccac gtaggaaggc
     1981 ggaatccaat ccatgaccct cttcatcacg cccggccagt ccttgaggag gaaaaagaca
     2041 aagaaaggga cgaggatgag cgccataata aatggtgtcg cctgcatgag aatctctgaa
     2101 gcgctcagtg tccgttcgag aaatccgctc ggaccaaaca catcgtcaac aagttttgtg
     2161 atacttgact caatgaatgg gtacttttct gcagtgaaca agagggcgtc ctgtgtctga
     2221 cgcgaaaact ccggaaactt tgcataggaa ttcatgattt cctgcttaag atcgggaatg
     2281 aagataaggt ctgctgcaat cacgaacacg gtaacgagaa caaaaagcag aatgactgcc
     2341 acgctcctct tgattccaag tctttctaag gcgcagataa ccgaactgaa gatataggca
     2401 ataatcgccg cggctatcag catcatgagg aggtgagtaa tacgcgaggc gacgactgct
     2461 gcgacaccga aaaccaggag cgcaagaacc catcttggca tctggtgacg gagcttgcgc
     2521 agatcggtca gcgaggtgcc acccttatgt ctttcctcat tcatgatgac atcctttcat
     2581 tcttcattcg ggctggtcac gttgaatgta cttagataaa atctcggcga ggttcaatag
     2641 aattctggaa gagattgcac tgttgccccg gatgagatcg tcaaaatatg atttatacat
     2701 gatcaaaagg agcgaatcct tttcagcgac agcagtcgca aaacgggaac actggccaat
     2761 aagggagagc tcaccaaaat gggaccctgg ccccagaaca gcaagtgtct tgctctctga
     2821 attcgacttt ttgattatct tgaccgatcc ctcgatgacg acgtagaccg cctttccgct
     2881 ttctccctca tgaaagatga tatcgcccgc tgtgtatttt ttttcgatga ggtcgataag
     2941 cagtttcctg agtagcttct tgcccatacc tgtaaagagg ggaacgtcac tcagtgtgcg
     3001 gagatgggat tccctggttg tatccagcgc ccaggtaaaa tatctcctca ctattttgtt
     3061 atctaggacc atggattatg gattctaccg cattcacgcg gttcctcgca acatgtattt
     3121 ccattataga taaagcgtaa ccccgaaaga agaatctgaa aatgaaaggg atgggctcac
     3181 tcaggcgcgc gcgaacaaga atatctttac atccccttct tgtatctcag atattctatg
     3241 agacctggga ggcttcctgt ggctacggag agaaaccacg caaaagagac cgcagtcgcc
     3301 tgggccggtt ttatcccgag ggaaccgaaa agtaggacaa aggaggcctc tctcaccccc
     3361 aggcctgata tggagatggg aaccgaggag atggtggcta tgatgggaac gaatataaaa
     3421 agtggaagca gggggacatc gaccttcaat ccaaggctga gcacgtacac ggcaaaaatg
     3481 atgactacct gcacgataag agaaagaaag aacgttttca gtattactgt ctttttccct
     3541 ttatagaggg aaaaatagtc ataaaatcct gacaggagcc ttatcctcct acccaccttg
     3601 aggccgaata taacaaagct ccccaaaata aagaggaata ccataagggg cagaatccat
     3661 tctatatacg accccctgaa gtatctcaat ccaaagggga aggcggttaa tcccacacac
     3721 ataagtgcgg tgaatccgat atacctgtcc ataaataccg aggcaatggc aggcgcgcct
     3781 ttgccggtat ccttgtaaag atagtatgcc tttaccgcgt ctccgccaat aatccccgga
     3841 agaagatggt taaaaaaaga ccctatgaaa tagagggaga agagttttct taactcaaac
     3901 tcttcatgga gcagaagccg ccaccgcacg gaggataggt agatggcgaa aatatagatg
     3961 aaaacagcaa gaaagaacgc gaggggattt atgcccttga gtgtggaaat gaccttatca
     4021 gcacctgtct tcgaaatgac gaaatagagc agtcctgaac tcaccaggag ctttccgagg
     4081 atgactatga gcttatttgg acgggccaag gaccttcttt atgacataga tgggtttacg
     4141 ctgtgtctcg tgatagaccc tcacaatcat ctcacccaga agccccatgc cgataaattc
     4201 cacaccaaca atcaagagca gggctcccag aaggagcaat ggtctccccc cgattggatt
     4261 gccaaaaatg accttatcga tgctcagata aaacaatatc aagaatccta tggccccccc
     4321 ggccataccg atggaaccaa aaaactggag cggttttgtg gaaaaactct ggagaaactt
     4381 gaccgtgata aggtccagaa ccacttttat ggttctcgtg ataccatatt tcgattttcc
     4441 cctgagacgc gggtggtgca tggtctccac ctcggcaacc cttaccccat accagctcgc
     4501 tactgcagga atgaaccggt gcatttcacc atacagtttg agattcttta tgacgtccct
     4561 tctgtatgct tttagggagc atccgtaatc atggagcttg acgcctgtga c
//
LOCUS       DATKOU010000213         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2615071, whole genome shotgun sequence.
ACCESSION   DATKOU010000213 DATKOU010000000
VERSION     DATKOU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615071"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VMU21_10010"
     CDS             complement(<1..1088)
                     /locus_tag="VMU21_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HUO77896.1"
                     /translation="MKKHLVRVILLLAASIMCASSVYAEVKFDYGADFRLRQEIWDDV
                     VTLDTLALTSSGADRNFLRLRTRLWGKADFSNDFGAYARLTNEMKWYGLGPYDSFKYN
                     PDNDKFDPDEIIIDNLYFDAKNLFGAVDLRIGRQDFIGTYGEGFLLMDGTPQDGSRTF
                     YFNAAKANVKVSQNFNFDVTYITDPKTDIYLPSIHPSVTGFPGNAYQFVNNKKVLPAS
                     NEQAALFYGRGKIDNLTVEPYYIYKTEKGLAAGGIDPTRLNLHTFGARALYSIDKWNM
                     GGEFAYQFGEYDDNLDRTGAGGYVFVGRKYDAVMWKPEFELRYVYLSGDKAGTKDKVE
                     TWDPLFSRNPYWNELIIYTLIPETSKYGG"
     gene            complement(1349..1765)
                     /locus_tag="VMU21_10015"
     CDS             complement(1349..1765)
                     /locus_tag="VMU21_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77897.1"
                     /translation="MKTAYAVRVAQKETRIVCSVLMVITVTCGILLTPVIMVSLVTAQ
                     DSKPTAVINGDSNVSVRQTVYLDGTMSSDPNGLGLNFRWTLVSIPEGSTAVLADDTNS
                     QVKFEADAVGIFKVKLVVNNGLVDSTPAYYTITVTK"
     gene            2016..2813
                     /locus_tag="VMU21_10020"
     CDS             2016..2813
                     /locus_tag="VMU21_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUO77898.1"
                     /translation="MSGIVDFHTHAFPDALAERAIKTLEEEGGIKAHLDGRVSSLLSS
                     MDTHGIEKSVVCSIATKSSQFDPILKWCKEIRSERIIPFPSFHPADPDFRQRIIEIKG
                     EGFKGIKFHPYYQDFAIDEERLFAVYEEICRTNLMLVVHTGFDIAFPRVQKADPHKIV
                     EVKQRFPELKLVTTHLGSWEQWKEVEEQLMGKPIFMEISCSLESLDKNRAREIILNHP
                     HNYILFGTDSPWTGQGETLSLFQELNLGAEMENRILRENAANLLNSV"
     gene            2915..>3617
                     /locus_tag="VMU21_10025"
     CDS             2915..>3617
                     /locus_tag="VMU21_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase"
                     /protein_id="HUO77899.1"
                     /translation="MKSKDDKKEKRLLSGNEAIARGAYEAGVKVASAYPGTPSTEILE
                     NLSTYKGIYTEWAPNEKVAVEVALGASFAGVRALASMKHVGLNVAADPLFTAAYTGVR
                     GGLVIITADDPEMHSSQNEQDNRNYAFAAKVPMLEAADASEAKELIKTAYRISEEFDT
                     PVLFRTTTRVSHVKGIVSIGDMETSAVNPGIEKMPEKFVMLPAHARKRRIELEKRMVR
                     LREFAETFPGNRIEWG"
BASE COUNT          967 a          819 c          871 g          960 t
ORIGIN      
        1 ccgccgtact tagatgtctc cggtatgagg gtatagatga tgagctcatt ccagtaaggg
       61 ttcctcgaga agagagggtc ccatgtctcg accttgtctt ttgtgcctgc tttgtcaccc
      121 gagagataga catatctcag ctcgaactca ggcttccaca taacggcatc atacttgcgg
      181 cccacgaaga catatccgcc cgctccggtc ctgtccaggt tgtcatcata ttcgccaaac
      241 tggtaggcaa attcaccccc catattccat ttgtcaatag aatagagagc ccttgcaccg
      301 aaggtgtgca gatttagcct ggtgggatca atgccgcctg ctgctaatcc tttctcggtt
      361 ttgtaaatat agtaaggttc gacagtgaga ttgtcgattt taccccttcc gtaaaacaga
      421 gcggcctgct cgttggaagc cggcagcacc ttcttgttgt tgacaaattg atatgcattg
      481 ccggggaagc cggtgactga tgggtgtatc gaaggcaaat aaatatcggt ctttggatca
      541 gtgatatagg tcacatcaaa attaaagttc tggctcacct tcacatttgc ctttgcggcg
      601 ttaaaataga atgtcctcga accgtcctgc ggcgttccat ccatgagcag gaagccttcg
      661 ccgtatgttc ctataaagtc ctggcgtccg atcctgagat caacagcgcc gaagaggttc
      721 tttgcgtcaa aatagagatt gtcgatgatg atttcgtccg gatcaaactt atcattgtcg
      781 ggattgtact tgaaggagtc ataggggcca agtccgtacc acttcatctc attggtaagt
      841 ctcgcgtatg cgccaaaatc attgctgaaa tccgcttttc cccagagccg cgtcctgagg
      901 cgaagaaagt tccggtccgc gccgcttgat gtaagcgcga gcgtatcgag ggttactacg
      961 tcatcccaga tttcctgtct caatctgaag tccgctccat aatcaaactt gacttctgca
     1021 tagacagatg aggcacacat aatgcttgct gccaatagca gaattactct gaccagatgt
     1081 tttttcatcc ttgttccctc cctttttgaa ttttctctaa aggcttctgc aatgtataat
     1141 tcaggaatcc ctttttcact caacggagac cttcaacaaa agaacgttgt tttattcttt
     1201 gcgagaagaa cagtttttgc caaggaattc cgtgattaga cgaaataata agccgtttgg
     1261 aaccatcagt tcaaatatat tatcctaata aattgataag aaatattaat aaataatata
     1321 gatgagcaga gttacttgca tgtcccgatt atttggtgac tgttatggtg taataagcag
     1381 gggtgctgtc caccaatccg ttgttcacga cgagcttcac cttaaagatt ccgaccgcat
     1441 cagcttcaaa tttaacctga gagttggtgt cgtctgcaag cactgcagtg ctgccttccg
     1501 gaattgatac caatgtccac cggaaattga gtccaagccc attgggatca gagctcatag
     1561 tgccgtcaag gtagacagtc tgcctcacag agacattcga gtccccattt atgacggcag
     1621 taggttttga atcctgtgca gtgactaagg agaccataat cacgggagtc aaaagaatgc
     1681 cacacgttac ggttatcacc atcagaaccg agcacactat cctagtttct ttttgcgcaa
     1741 ctcgtacggc atatgcggtc ttcatggtat tccctcctta aattgcttca tgtttttatt
     1801 ctggattaca tcatatacca aatcacgcca cttttgaagt ttgatgagat tatcatttat
     1861 agaagattgg ccctttatgc agcgttctgg atgggtggca ggtttgatcg gaatacgcgg
     1921 ggactctttg agtcacaagc tacttatgaa ttttactaca acaggcagat taccttataa
     1981 taagtcccta ataaagccat gagaaaggtt gtggaatgag cggaatcgtt gattttcata
     2041 cccatgcatt tcctgatgca ttggcggaga gggcaattaa gaccctggaa gaagaggggg
     2101 ggatcaaggc ccatctggac ggcagggtgt cttcgctcct ctcttccatg gacacgcatg
     2161 gtatcgaaaa aagtgttgtc tgctccattg caacaaagtc gtcccagttt gatcctatac
     2221 tcaaatggtg caaagagata cggtctgaga ggatcatacc tttcccttcc tttcatccag
     2281 cggacccgga ttttcgccag aggattattg agataaaagg ggagggtttc aaggggatta
     2341 agtttcatcc ctattatcag gactttgcca ttgatgagga aagacttttc gcggtttatg
     2401 aggagatttg caggacaaat cttatgttgg tagtccatac aggttttgat atcgcatttc
     2461 caagagtaca gaaggcagac cctcataaga tagtggaggt caagcaaaga tttcctgagc
     2521 tcaaactggt taccacgcac cttggctcct gggaacaatg gaaagaggtg gaggagcagc
     2581 taatggggaa accgatcttt atggagatat cgtgctccct tgaatctctg gataaaaatc
     2641 gtgcacggga aattattctg aaccatcccc ataattatat ccttttcggc acagactctc
     2701 cgtggacagg gcaaggggag accctctcgc tttttcagga acttaacctt ggtgcggaaa
     2761 tggaaaatcg tatcctgagg gaaaatgcgg ctaaccttct gaattcggtc tgattctata
     2821 gaaacctctg cagtcttaag tcaagccaag gcccggatgt tattgaaaat aacaccatgg
     2881 tatgataacc tacacttttg aaattttttg agaaatgaaa tccaaagacg ataagaaaga
     2941 gaaacgattg ctctcaggaa atgaggccat tgcccgggga gcgtatgagg caggagtgaa
     3001 ggtcgcctct gcgtatcccg ggaccccctc aacagagata ctggaaaacc tctccacgta
     3061 taaagggata tacacagaat gggcccccaa tgagaaggtg gctgtggaag ttgcgctggg
     3121 ggcctctttt gcgggagtga gagccttggc ctcgatgaaa cacgttggcc ttaatgttgc
     3181 agctgaccct ctctttaccg ctgcgtatac gggagtgagg ggtggcctcg ttatcattac
     3241 cgctgatgat cccgagatgc atagttccca gaacgagcag gacaacagga attatgcatt
     3301 tgccgcaaaa gtccccatgc tggaagcggc tgacgcttct gaagcaaagg aactgatcaa
     3361 aacggcctac aggataagcg aggagttcga taccccggtg cttttcagga ccaccacgag
     3421 ggtctcccat gtaaagggca ttgtctccat tggtgatatg gagacgtctg ccgtaaatcc
     3481 tgggatcgaa aagatgccgg aaaaattcgt catgcttcct gctcacgccc ggaaaagaag
     3541 gatcgagctg gaaaaacgta tggtcaggct cagggaattt gcagaaactt tcccgggaaa
     3601 cagaattgaa tggggaa
//
LOCUS       DATKOU010000214         8206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2615088, whole genome shotgun sequence.
ACCESSION   DATKOU010000214 DATKOU010000000
VERSION     DATKOU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615088"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VMU21_10030"
     CDS             <1..406
                     /locus_tag="VMU21_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77900.1"
                     /translation="MGVSDVGGDVVAVASQTVKGAVKGAAEVGADVAMVARRTVDGVI
                     EATKEIGGNVEEVAKVAVGGAIEAAGTIGNTAVQAVKDMLVGVVEGVKEIGSAALPKP
                     GMEKGSPRAHSSFAEGEIAEALMKKTKQKKPK"
     gene            504..734
                     /locus_tag="VMU21_10035"
     CDS             504..734
                     /locus_tag="VMU21_10035"
                     /inference="COORDINATES: protein motif:HMM:NF023507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3562 domain-containing protein"
                     /protein_id="HUO77901.1"
                     /translation="MATSCLYDNDTDRRLHSSAIRMLAREFNVPEEEIQALYETMLSS
                     LKESARIKDYLAVLVSRNVKDMIRRGVSSQTS"
     gene            768..902
                     /locus_tag="VMU21_10040"
     CDS             768..902
                     /locus_tag="VMU21_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77902.1"
                     /translation="MRGFGVHPAVQKRCIAMEKQERESSVIIVSFVKCNSRVAQKEPL
                     "
     gene            1211..1951
                     /locus_tag="VMU21_10045"
     CDS             1211..1951
                     /locus_tag="VMU21_10045"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUO77903.1"
                     /translation="MEIVAVANHKGGVGKTTSAVNIAACWGEMGQTVLLVDLDPQGSA
                     SMSFGIENDGTEILQALQKTIALPVVGTQAEGVALVPAGPHLATARQRFSGSIGKELL
                     LRCLKQMQDGWEKVIIDCPPSLGILTMAALWASNHVIIPVEANYLAMSGLRQMVDTVE
                     SVGNDHPGIAIRAVIPCRAHPRRRVHQAIMEQFEQLFPEKVSPIVRENAALAEAPGRG
                     RPVILSAPKSHGAEDYRQVALWLSEHIA"
     gene            complement(2293..2769)
                     /locus_tag="VMU21_10050"
     CDS             complement(2293..2769)
                     /locus_tag="VMU21_10050"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HUO77904.1"
                     /translation="MKKTQVLFVCIHNSARSQMAQAFLNTIAGDRFIAESAGIEAGVL
                     NPLAVEAMAEVGIDISQNTTRRAFDLLKSGRHFQYVITVCDAVNAQKCPVFPGAIQHI
                     HWSFPDPAQLQGTHDEKMKEVRKIRDSIKEKIEWWISETKAGKDPEIEIPPFKFGV"
     gene            complement(3063..3272)
                     /locus_tag="VMU21_10055"
     CDS             complement(3063..3272)
                     /locus_tag="VMU21_10055"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HUO77905.1"
                     /translation="MSIVQMNAQKKIALVAHDNMKKALLEWARYNKELLAQHELYATG
                     STGTLLSRALGVEVHCFKSCPLGGD"
     gene            complement(3470..4234)
                     /locus_tag="VMU21_10060"
     CDS             complement(3470..4234)
                     /locus_tag="VMU21_10060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO77906.1"
                     /translation="MMLPHQKKWILQEFYFRYIHPSALRCLRGAALLEYPREYLDFLN
                     DRTAKHHYGNWDEIYIRIGSTKPDYDLWLEKYAGILKDSTDIPIIDLGCGYGNDTLYL
                     HERGYKVVSCDLSMEALKRLNHFIDKPVIRHFDVLAGLPFDDGAARIIIADLSLHYFY
                     WADTQRIIKDIWRVLSKGGYLLCRVNSIRDNNGAGEGRQLEEDYYEIRGRQKRFFSQG
                     SLEELFRDWYITYLCEYSLYRFGKEKMLWEVAAQKE"
     gene            complement(4596..5960)
                     /locus_tag="VMU21_10065"
     CDS             complement(4596..5960)
                     /locus_tag="VMU21_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUO77907.1"
                     /translation="MNQTDCVESLNPEASSPGSQKSALSDDHRNVRNIFVLDTNVLIH
                     DPNSIFSFDEHDVVIPFVVMEELDRLKKGHGEIAASARTALRIIDQVMEKGSLISGIK
                     MKTGGLLLIGTTLSSAPTVFMDGVTEDNKIIRTALGIVEKQQTDERGNIPSVTLVSKD
                     TTVRIKAEACGLHAGDYESDKTTLFQKYGRVLEESDYTNGIHSIRYLRTGEDIFRLQG
                     SDNHTKIKNGKSLEGISPKNVEQRCAIDALTNPDIEVLALTGSAGTGKTLLALAAGMH
                     QTTKKSPLYEQVVVARPVVPMGNDIGFLPGDVSEKLAPWMQPVFDSLEVIINTPKGHI
                     KDESSASQYRSFQYLIDTGVLQIEALTYIRGRSLPLRYFIVDEAQNLRPIDVKTIITR
                     CGEGTKIVFTGDLNQIDTPYLDAKSNGLAYLISRFINEENFCYLNLSCSVRSRLAEQG
                     ARLL"
     gene            complement(6214..6915)
                     /locus_tag="VMU21_10070"
     CDS             complement(6214..6915)
                     /locus_tag="VMU21_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO77908.1"
                     /translation="MSSKNLFVVDDEADLVELVSYNLKKEGFAVDSASDGEEGLSKIR
                     KKNYDLLILDLMLPGLQGMELCRILRNDPKTAGLPIIMLTAKGEEVDKVLGLEMGADD
                     YITKPFSPRELVARAKAVLRRSPGERSPEQGILMIGGLEIDREKYVVSIKGKPIKPSA
                     TEFKLLLYLVERKGRVFSREQLLDAIWSDEAFVEPRTVDVHIRRLRAQIEDDPANPRY
                     IKTLRGIGYFFAEKE"
     gene            complement(7136..8125)
                     /locus_tag="VMU21_10075"
     CDS             complement(7136..8125)
                     /locus_tag="VMU21_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate dehydrogenase"
                     /protein_id="HUO77909.1"
                     /translation="MKPKVVITHWVHPEVIEFLSQHCEVVPNLTHETLPRGEIIRRSE
                     EAQAIMVFMPDSIDRDFLEACQDLKIVAAALKGYDNFDVDACTRYGVWLTIVPDLLTV
                     PTAELTIGLLIGLSRNMLQGDRYVRSGKFKGWRPEFYGTGLTGSILGIIGMGAVGQEI
                     ARMLLGFEMNVLYNDTVVLTEEENEELGVTYASFEYLLQNSDFVVPMVPLTSATKHMI
                     NKKTIAQMKPGSFLINTGRGSVVDEQAVANALASGHLGGYGADVFQMEDWARIDRPLG
                     VPRELLENVEKTFFTPHLGSAVDSVRREIAMEAARSILQALDNEMPNGAVNQP"
BASE COUNT         1984 a         2072 c         1917 g         2233 t
ORIGIN      
        1 catgggtgtg agtgatgtcg gcggagacgt tgtcgctgtt gccagccaga cagttaaagg
       61 tgcggtaaag ggtgcagcag aagtgggtgc ggatgttgcc atggtggcaa gaaggacggt
      121 tgacggagtt atcgaggcca caaaagaaat tggcgggaat gtggaagagg tggcaaaagt
      181 ggctgtggga ggagcgatcg aagctgcagg caccatcgga aatactgccg tgcaggccgt
      241 aaaagacatg ctcgtgggtg ttgtagaggg cgtaaaggag atcggaagtg ccgctcttcc
      301 gaagccagga atggaaaagg gatcacctag ggcacattcc tcttttgccg aaggggaaat
      361 cgcagaagcc cttatgaaaa agacgaagca gaagaagcca aaatgaacgt catggctccg
      421 cgagttgcag ctggaggcgg gcggcaagac acagattatt gacgaagata cctactgccc
      481 gccttttgat aagaggtggt ataatggcaa cttcctgttt atacgacaat gatacagatc
      541 ggaggctgca ttccagtgca atcagaatgc tcgcaaggga attcaatgtt cctgaggagg
      601 aaatacaagc actttacgag acaatgctca gcagtctgaa ggaaagtgca cggataaaag
      661 actatttggc agtactggtc agccgcaatg tgaaggacat gatcaggagg ggcgttagtt
      721 cgcagacatc gtgaaggcaa cccaactctg tcagacagcg gaggtgcatg aggggattcg
      781 gtgtccatcc tgccgttcag aagcggtgta tcgctatgga aaaacaggaa agggaaagca
      841 gcgttattat tgtctccttt gtgaaatgca attcacgggt tgcacaaaaa gaacctttgt
      901 gagcagaagg cctctctgcc cggtctgtgg taatcccatg catctctata tgagaagcgg
      961 cgagagatac cgctttcgct gctcaaagta tcctcagtgt ggggcattta tagtgatgat
     1021 ggtaaaccgt gaaagtgagt cggataggcc gatcatttcc tgtccctgaa aaaaagaccg
     1081 caaaagattc atgaaaaaac tttttcccct cacggacggg tcacgtagtt gacatttgct
     1141 cttgtctcga agcaaacttt acatattgcc gtgtcttgct gcacctggca aagcggagag
     1201 aaaagaattc atggagatcg tggcagtagc aaaccataag ggtggagtcg ggaagactac
     1261 ctcggcagtc aatattgctg cctgttgggg agagatgggg caaacggttc tccttgttga
     1321 tctcgaccct cagggaagtg caagcatgtc gttcggcata gaaaatgacg gaaccgagat
     1381 ccttcaggca ttacagaaga ccatagcatt gcctgtagtg ggtactcaag ccgaaggcgt
     1441 ggccctggtt cctgcagggc ctcatttggc gaccgccaga cagagattct ccgggtcaat
     1501 aggcaaggaa ctgctgcttc gttgcctgaa acaaatgcag gatggttggg aaaaggtaat
     1561 tatagattgc ccgcccagcc tggggattct caccatggct gctctgtggg caagcaatca
     1621 tgtcattatc cctgtcgaag caaactattt ggcgatgagc ggccttagac agatggtgga
     1681 tactgttgaa tctgtgggaa atgatcaccc tggcatcgcg atcagggcag tgataccctg
     1741 ccgagcccac ccaaggcgca gagttcatca ggcgatcatg gaacagtttg aacagctttt
     1801 cccggagaaa gtctcgccaa tcgtccggga aaacgcggcc ctggcagagg ctcctggacg
     1861 cggtcggccg gtcatacttt ctgcccctaa atcccatggg gctgaagatt accgacaagt
     1921 tgcattatgg ttgtcagaac atatcgctta aatcagataa tagagactat tctctatatt
     1981 tttggttgtc gaaatttaaa tagccatttg gtctcgaaaa tagcctttct gttcctttaa
     2041 ttgcgtatcc cggcgattag gtacacccat tccgtctcgc gccggcgccg ttcctgagta
     2101 atggggcacc tattccgaac gattggggac agttttagag aacctttctt gtcatcgtat
     2161 cacttcgaga cagttcttta tgtcgtgttc accgactttt ccgcactttc aagaaagcga
     2221 aacttcacga agaatacgac tgcaatagga caccttcgaa aaaaccagaa gtgcgtttgc
     2281 ttcagccaga cttcacacgc cgaacttgaa gggtggaatc tcaatttctg ggtccttacc
     2341 tgctttggtt tcagagatcc accactcgat cttctccttg atggaatctc gaatctttct
     2401 gacctctttc attttttcat cgtgagtgcc ctgtaattgg gcagggtcag ggaagctcca
     2461 gtgaatatgc tgtatggcgc ctgggaatac cgggcacttc tgtgcgttaa ctgcatcgca
     2521 gaccgtaatt acatattgga aatgtctgcc gctttttaga agatcaaagg cccgccgagt
     2581 ggtattttgt gagatgtcaa tgccgacctc agccattgcc tcgactgcga ggggattcaa
     2641 aacgcccgcc tcaatacctg cgctttcggc tataaagcgg tcgcccgcga ttgtattcag
     2701 aaaagcctgt gccatctggc tacgcgcgct gttatggatg catacaaaca agacctgtgt
     2761 ctttttcatt cctacctcct tatgttttgt cgatgcgttg taaaggtcac aaacccctca
     2821 tggggtatta caccttattg ccctcccagt tagggtctgc acctcccgac gcatcttatc
     2881 ttcttagaaa actccatgat actgcccctg gaaggcttgc caagtatgaa agcattaagc
     2941 ctctccctgt cctctgccat ggcgttcttc ggcatccttt cgaagacaga tagaagcaag
     3001 gatcttctga agaaataaga atcatcgccg atctcgcctc ctgcaatctc gaccccaatc
     3061 tgctaatctc cgccgagggg gcagctctta aagcagtgta cttcgacgcc caaagccctg
     3121 gaaagcaggg tcccagtgct tccggtagcg tacaattcat gttgagccaa gagttccttg
     3181 ttatatcttg cccactccag aagcgccttt ttcatgttat catgagcaac aagagctatt
     3241 ttcttttgcg cattcatttg aacaatactc ataatccctc catacttttt tgttgtaatt
     3301 gtacaatcgg tattttaagg cgtgatgtca aatgtaacct atccgccatt atgcagtcat
     3361 ctgcccaggg cagtcttcgc acagattccg ctttttaata aactccttct gacgttcacc
     3421 tagactgttg cgtttcgcct tgcacctgtt cgcctttatc gcagtatctc tattccttct
     3481 gcgcggcaac ttcccatagc atcttctctt tcccgaatct gtataaggag tattcacaca
     3541 gatacgtaat ataccaatcc ctaaaaagct cttcgaggct gccttggcta aagaaccttt
     3601 tttgccgccc ccttatttca taatagtctt cctccaactg ccgcccctct cctgctccat
     3661 tattatccct gatggaattc accctgcata aaagatagcc gcctttacta agtaccctcc
     3721 atatgtcctt aataatgcgt tgcgtgtctg cccagtagaa gtaatggagg gagagatccg
     3781 cgataatgat cctggctgca ccatcatcga acggcaggcc agccagcaca tcgaagtgtc
     3841 tgataaccgg cttgtcaata aaatgattca gtcttttaag tgcctccatt gagagatcac
     3901 aggatactac tttgtaccct ctttcgtgaa ggtacagggt atcattgcca tagccgcaac
     3961 cgagatcaat aatcgggatg tctgtagagt ccttcagtat gccagcgtat ttttccaacc
     4021 agagatcata gtcaggcttt gtactgccga tgcgtatata tatttcgtcc cagttcccgt
     4081 aatgatgctt tgctgttctg tcgttaagaa agtcaagata ttcacgggga tattccagca
     4141 gggcagcgcc tctgagacaa cgcagagcgg agggatggat gtagcggaaa tagaactcct
     4201 gtaatatcca ctttttttgg tgtgggagca tcattatatg aggggaagcg agctcatttc
     4261 acctccttca ttataaattt tttgaaagtc ttgcagacag gatagccgga gcacctgaac
     4321 ctgatgacat cactctccag cttataaaca ttcatcggct ttctgcatgc aggacatgcc
     4381 ggtttgcctt taaccggtga cttctttgcc atagggttaa cccgagttcc ccacattaca
     4441 catataaatc gttattttcc cttccttatc ttcccatatc ttaatacctc gctgctgtca
     4501 caggacgggc atctagcttt agtcggttcc accatagttt aatgatcctt tgttaaaaca
     4561 tgatggttga aatgttaaat tcctgtcaca taaattcaca gaagccgtgc cccctgttca
     4621 gcgagccgcg atctcacact gcagctcagg ttcagatagc aaaagttctc ttcattaata
     4681 aacctgctta tgagatatgc cagtccgttg ctttttgcgt cgagatatgg cgtgtcgatc
     4741 tgattcagat cgcctgtgaa gacaatctta gtgccctccc cgcaccttgt gataattgtc
     4801 ttgacatcga tgggccttaa attctgcgcc tcatccacaa tgaaatacct gagcggcagg
     4861 cttctccccc gtatatatgt cagggcctcg atctgcagta cgccggtatc tatcagatac
     4921 tgaaaactcc tgtactgact tgctgagctt tcgtccttaa tatgaccttt cggcgtgttg
     4981 atgatcacct ccaggctgtc gaatactggc tgcatccagg gagcaagttt ctcgctcaca
     5041 tctcccggca ggaagcctat gtcattaccc atgggaacaa ccggtcttgc caccaccacc
     5101 tgctcgtata aaggtgattt cttcgttgtc tggtgcattc ctgcagccag cgcaagaaga
     5161 gttttccctg tgccggctga gcccgtgagt gcaagaactt ctatatccgg atttgttaat
     5221 gcgtctatgg cgcagcgttg ttccacattt ttgggggata tgccttcaag cgacttgccg
     5281 ttcttgatct ttgtgtggtt gtcactgccc tgtaacctga agatatcttc accagtcctc
     5341 aagtatcgta tcgaatgaat gccgttggtg taatccgatt cttccagcac tctgccatac
     5401 ttctggaaaa gcgttgtctt atcgctttca tagtctcctg catggaggcc gcatgcctcc
     5461 gcctttattc tcacggtagt gtctttggac accaaagtga cagacgggat atttcctctt
     5521 tcgtcagtct gctgcttttc cacaatgcca agggccgtcc ttatgatctt gttgtcctct
     5581 gtgacgccgt ccatgaaaac agtaggagcg ctgcttaacg tagtgccgat caaaagcagg
     5641 cctcctgttt tcatcttgat cccggagatc agtgatccct tctccattac ctgatcaatg
     5701 atcctgagcg cagttcgtgc tgacgccgct atctccccgt gccccttttt gagcctgtca
     5761 agctcttcca taacaacaaa cggtattacc acgtcatgct cgtcaaaact gaagatggag
     5821 tttggatcgt gaatcaacac attcgtgtcg agtacgaata tgtttctcac gttccgatga
     5881 tcgtcagaca gggccgactt ctgtgatccc ggcgaagatg cttcggggtt aagagactcc
     5941 acgcagtctg tttggttcat gcaggtcctc cgttctgata ataattattg ttgtgggaat
     6001 taggtgtgcg cgaagcaagg caggaagaac caagaaatta ttcacaagaa catttcttga
     6061 taatatttat gttgtaactc aagctctcta tcattctcct aaaaacattg tgacattgtt
     6121 ctgttacagg attgttacag tctcatgaaa ttttagtgac cgactctatg gcatctcatt
     6181 tgagtttcgt aatatgctta atgatggtac ttttcattcc ttctctgcga agaaatatcc
     6241 gatgccccgg agggttttga tgtatctcgg atttgcagga tcatcctcga tctgggccct
     6301 caaccttcgt atatgcacat caactgtcct gggctccaca aatgcctcgt cactccatat
     6361 tgcgtcaaga agctgctccc tgctgaatac ccttcccttt ctctccacaa ggtagaggag
     6421 gagcttgaat tccgtggcac tgggtttgat gggttttcct ttaatggaga cgacatattt
     6481 ctccctgtcg atctcaagcc cccctatcat cagaattcct tgctcggggg acctctcccc
     6541 gggagacctc ctcagtactg cctttgctct tgccacaagt tccctgggac tgaagggctt
     6601 tgtgatgtag tcatccgctc ccatctccag cccaaggacc ttgtccactt cctccccctt
     6661 tgccgtaagc atgatgatag gcagaccagc agtcttggga tcattcctga gtatcctgca
     6721 gagctccatg ccctggaggc caggcagcat gaggtccagt atcaggaggt cataattttt
     6781 ctttctgatt tttgagagcc cttcttcccc gtctgatgct gaatcgacgg caaagccctc
     6841 tttcttgagg ttataagaga caagttcgac aaggtcagct tcgtcgtcga ctacgaataa
     6901 attctttgag ctcatcagca ataccttatc tggaatcttt gcaggctctc gcgtgacgta
     6961 atgagttccc ccactcgggg cgatgcgacg ctaaaagaat ccattgttat gctcctttca
     7021 tgcggccaat catgcagcaa tctcagatgt atttctgccg acttcattca tccttctcag
     7081 acactcatca ctcactttaa tctctttcct gtttccgccg gatattcgaa ttttctcagg
     7141 gctgattgac ggctccgttc ggcatttcgt tgtccaaggc ctgaaggatg cttcgtgccg
     7201 cttccatagc tatttcacgc cttacagaat caactgcgga accgaggtga ggggtaaaaa
     7261 aagttttctc cacattttct aggagttccc gcggaactcc cagtggacga tcaatccggg
     7321 cccaatcctc catctgaaat acatccgcgc cataaccgcc aagatgccca gaggccaggg
     7381 cattcgcaac tgcctgttcg tccactactg agccgcggcc ggtattgatg agaaaactgc
     7441 cgggtttcat ctgcgcaata gtcttcttat tgatcatatg cttggtggcg gaagtgaggg
     7501 gcaccatggg gacaacaaaa tcactgtttt gaagaaggta ttcgaacgag gcataagtta
     7561 ctcccaattc ttcgttttct tcttctgtga gaaccacagt gtcgttataa agcacattca
     7621 tttcaaatcc caacagcatc ctagctattt cctgaccaac tgcacccatt cctatgatgc
     7681 caagaattga gccggtaaga cccgtcccat agaactccgg tcgccagcct ttaaacttac
     7741 cgctgcgtac ataccggtca ccctggagca tgtttcggga cagacctatg agcagaccta
     7801 tagtcagttc tgcagtggga acggtcaaaa gatcggggac aattgttaac cagacgccat
     7861 acctcgtgca ggcatcgacg tcgaagttgt catagccctt taacgcggca gcaacaatct
     7921 tcaaatcctg acaggcctcc agaaagtctc tatcaatgct gtcgggcata aagaccataa
     7981 tcgcctgcgc ttcttccgac cggcggatta tttcccctct tggcagggtt tcatgagtga
     8041 ggttcggcac aacctcgcaa tgctgactca ggaactcgat gacttccgga tgtacccagt
     8101 gtgtgatgac tactttcggc ttcatgtatt tgctcctttc tactacttga atcgcttacg
     8161 caaggacgag cctaacccgt caacgagggt gaccattaag agaatg
//
LOCUS       DATKOU010000215         4402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1081238, whole genome shotgun sequence.
ACCESSION   DATKOU010000215 DATKOU010000000
VERSION     DATKOU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081238"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VMU21_10080"
     CDS             complement(<1..791)
                     /locus_tag="VMU21_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HUO77910.1"
                     /translation="MKIFKKVLVANRGEIAVRVIRACRELGIRTVAVYSDIEKDSMHV
                     RLADEAVCIGPANSLQSYLNIPGILSAAEITDSEAVHPGYGFLSENPHFAEACATSGI
                     TFIGPSAENIRLGGDKAKARQVMKRKGVPVVPGSDGPVLSEEMAIKVAKKIGFPVILK
                     ASAGGGGKGMRVVNEEKDLEQAFYLAQREALTSFGNSELYVEKYIPDMRHIEVQIMAD
                     DKGNVIHLGERDCSVQRRHQKLIEEAPSPISTEKFRRKIGELGIKA"
     gene            complement(788..1222)
                     /gene="accB"
                     /locus_tag="VMU21_10085"
     CDS             complement(788..1222)
                     /gene="accB"
                     /locus_tag="VMU21_10085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HUO77911.1"
                     /translation="MELDDLKELISFLKDTDITELQVEKEGIKVKIRRQMMLSSLEVH
                     AKPPLTTIERTGESEEDIQRLITITCPIVGTFYRAPSPDAPPFADAGTRVKKGQVICI
                     IEAMKLMNEIESEVDGVIVRALVENGQPVEYGEPLFLVEPVS"
     gene            complement(1222..1782)
                     /gene="efp"
                     /locus_tag="VMU21_10090"
     CDS             complement(1222..1782)
                     /gene="efp"
                     /locus_tag="VMU21_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HUO77912.1"
                     /translation="MISTNEFRKGAKIEYKGEPFEIIDFQHVKMGRGGAIVRTKLKSL
                     KSGSVLEDTFRSGEKFESPHLDERSMQYLYAEGDHHYFMDTETFEQIPLSTSQLGDAK
                     KFLKENMVVKILNYRGDPLTVEISTFVELVVAQTDPGYKGDTASGGSKPATLETGAVV
                     RVPLHINTGDVIRVDTRTSEYIERVK"
     gene            complement(1809..2918)
                     /locus_tag="VMU21_10095"
     CDS             complement(1809..2918)
                     /locus_tag="VMU21_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HUO77913.1"
                     /translation="MFQPFSSRLERLRDSLERKRIHAFLATNIVNVRYLSGFKGSSGA
                     VLITKEKNVFITDFRYQEEAGRELTGIPWELSIEKGDRLRIIKEMISDLGIRTLGFES
                     GVSYEFFQGLSRCGARLSPLTGIVESLRTIKDHGEIRSIKEAVRKAEEAFLDVKPYIR
                     PGRREREIALMLEARLKKRGCNHLPFDIIVASGVNSAMPHAKASDKKLSSGELVVIDW
                     GGEAEGYCSDMTRTFLLRGTGCHGGRNVGVDISRKEEIYQTVYKANRRAVSSVCPGCK
                     SKGIDEVARNIIKNAGYGKFFGHGTGHGVGLEVHESPRITWTRSDTISENMIFTVEPG
                     IYIPGLGGVRIEDMVLVRPKGPEVLTRLPRDLEIL"
     gene            complement(2921..3352)
                     /gene="aroQ"
                     /locus_tag="VMU21_10100"
     CDS             complement(2921..3352)
                     /gene="aroQ"
                     /locus_tag="VMU21_10100"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HUO77914.1"
                     /translation="MKVLVIHGPNLNMLGKREPEVYGTLTLGEINRRIKAVAAETGIK
                     VSILQSNSEGEIIDAIQKADYDALIINPAAYTHTSIAIRDAIAAVARPTIEVHISNVY
                     KREEFRRRSYIAEVSQGQITGFGAESYVLALHALKNILSRG"
     gene            complement(3443..3799)
                     /locus_tag="VMU21_10105"
     CDS             complement(3443..3799)
                     /locus_tag="VMU21_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77915.1"
                     /translation="MSFSEVLKDAVERVDGAVSAMILGSDGIPVEEYATEKLFSLEDL
                     GAEASAMIKDINLAAQELGLGVAREFSIISDRCGIIMRQINPGYYLALVIRPEGNYGK
                     GRYILKTAVPKLEGEF"
     gene            complement(3824..>4402)
                     /locus_tag="VMU21_10110"
     CDS             complement(3824..>4402)
                     /locus_tag="VMU21_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77916.1"
                     /translation="KEEIVFPLESPVEPLPDVEEIEAPAIETHTHEEETVVEPQAISH
                     EEFEKLEDSLSVETPETMPGIDEPIEIPEDQAIDLLEGEAFDDILAEPVEVPQEHPDL
                     SAPRMGEEAKPAGSGADIGAADSMVASGNFFKALEIYREVLSRNPDDKQTLQRILELK
                     TLMKLMGRGDEVVIAKLETFLDGVRRNFGKSQ"
BASE COUNT          994 a         1296 c          910 g         1202 t
ORIGIN      
        1 gcctttatac ccaactcccc aatcttcctt ctaaacttct ctgtggagat cggtgaaggg
       61 gcttcctcga taagtttctg atgcctcctc tgtacggagc agtcgcgctc gcccaggtga
      121 atcacattcc ctttgtcatc cgccattatc tggacctcga tatgcctcat gtccgggatg
      181 tacttttcca catagagctc actgtttccg aaggaagtga gggcctctct ctgcgcaagg
      241 taaaatgcct gctccaggtc cttctcttca ttcacaaccc tcatgccctt cccgccgccc
      301 cctgcagagg ctttcaaaat tacggggaac cctattttct tcgcaacctt aatggccatc
      361 tcctctgaga gcacaggtcc atcactcccc ggtaccaccg gcacaccctt ccgtttcata
      421 acctgcctcg cctttgcctt atcaccccca aggcgtatgt tctcggcaga aggcccgatg
      481 aaagtgattc ccgatgtggc acacgcctcg gcaaaatggg gattctcgga gaggaaacca
      541 tatcccggat gtaccgcttc tgaatcagta atctcggcag cgctgaggat cccggggata
      601 ttgagatagc tctgtaagga gttggcagga ccgatacata ccgcctcatc agcgagcctc
      661 acatgcatcg agtccttttc tatatctgaa tataccgcta cggtccttat cccgagttct
      721 ctgcaggccc ttataaccct gactgcaatc tcaccccggt ttgcgacgag gaccttctta
      781 aagattttca tgataccggc tcaacaagga agagtggctc gccgtactca acaggctgac
      841 cattttccac caaagccctc acaatcaccc cgtccacctc actctcgatc tcgttcataa
      901 gcttcatggc ctcgatgatg cagatcacct gacccttttt caccctcgtt cccgcatccg
      961 caaagggagg ggcatccgga gaaggtgccc ggtagaatgt cccgactata ggacacgtaa
     1021 tggtaatgag cctttggatg tcttcctcgg actcacccgt tctttcgatg gtggtcaagg
     1081 gaggtttcgc gtgcacctca agggaagata acatcatctg cctccttatc ttcaccttta
     1141 ttccctcttt ctccacctgg agttctgtaa tatcggtatc cttcagaaaa gagataagct
     1201 ccttcagatc atccagttcc attattttac cctttcaata tattccgatg tcctggtgtc
     1261 cacccttatc acgtctcctg tattgatatg aagagggact ctcaccacgg caccggtctc
     1321 caatgttgca ggcttggacc ctcccgaagc ggtatcaccc ttatacccgg gatctgtttg
     1381 cgccacgacg agttccacaa aggtagaaat ctctaccgtg aggggatccc ctctatagtt
     1441 gagaatcttt actaccatgt tttccttgag gaacttcttt gcatccccaa gttgggaggt
     1501 actgaggggt atctgttcaa atgtctcagt atccatgaaa taatggtggt ccccctctgc
     1561 atagaggtac tgcattgacc tttcatcaag gtggggtgat tcgaacttct cccccgaccg
     1621 gaaggtatcc tccaggacac tcccgctctt gaggcttttt aactttgtcc tcacaatagc
     1681 tccccctctt cccatcttca catgctgaaa atcgatgatc tcaaaaggtt cgcctttgta
     1741 ttctatcttt gcaccctttc tgaattcatt tgttgatatc aatacaccct cctcctgccc
     1801 tgcaaaattt agagtatctc cagatccctc ggtaagcgtg tcaacacttc aggtccctta
     1861 ggtctcacaa gcaccatgtc ttctatcctc acacccccga gccctggtat atatatcccc
     1921 ggctctacgg taaaaatcat gttttcactt atggtatcac ttcgtgtcca tgtgatcctc
     1981 ggtgactcat gaacctcgag acctacccca tggcccgttc catgaccaaa aaatttaccg
     2041 taccccgcat tttttattat attccttgcc acctcatcaa tacccttgct cttgcagcca
     2101 gggcaaacag aggagaccgc ccttctgttt gccttataga ccgtctggta tatttcctct
     2161 ttccgagaaa tatcaacgcc cacgttgcgt cccccgtgac acccggtacc tctcaaaagg
     2221 aatgtcctcg tcatatctga acaataacct tccgcttccc caccccagtc tatcaccaca
     2281 agttcacctg atgagagctt cttatcgctc gccttggcat ggggcattgc cgagtttacg
     2341 ccggacgcca ctattatatc aaaagggagg tgattgcacc ctcttttttt cagccgtgct
     2401 tccagcatga gggctatctc ccgttccctc cttccgggcc gtatataggg cttcacatca
     2461 aggaatgctt cttctgcctt ccgtactgcc tcttttatgg aacgtatctc tccatggtct
     2521 tttattgtcc tcagggattc tacaatacct gtcagaggac taagccttgc cccgcaccgg
     2581 gaaagcccct gaaaaaattc atacgatacc cccgattcaa aaccgagggt acgtatacca
     2641 agatcagaga tcatttcttt tataatcctt agcctgtcgc ccttttcgat ggaaagttcc
     2701 cagggaattc cagtcaactc cctcccggcc tcctcctgat acctgaagtc cgtgataaac
     2761 acatttttct ccttagtgat caggacagca ccggaggatc ccttgaaacc ggagaggtac
     2821 ctgacattta caatattggt ggcgagaaac gcgtggattc ttttcctttc aagggaatcc
     2881 ctcagcctct caaggcgtga tgaaaaaggc tggaaaatac ctaccccctg gaaaggatat
     2941 tcttcagagc atggagtgcg agaacgtagc tctcagcgcc aaacccggta atctggccct
     3001 gtgatacttc tgcgatgtaa gatctcctcc tgaactcttc cctcttgtac acattggaga
     3061 tgtgaacttc aatggtaggt ctggctacag ccgcaatcgc atcgcggatg gctatgctcg
     3121 tatgggtata tgctgcggga tttattatga gggcgtcata atctgccttt tggatcgcat
     3181 cgatgatctc accctcacta ttggattgca gaatagagac ctttatcccg gtttccgcgg
     3241 ccactgcctt gattctccgg ttaatctctc cgagggtgag cgttccgtat acctcgggct
     3301 ctcttttccc gagcatgttc aggtttggtc catggatgac taatactttc attggcagcg
     3361 ttcttagact atcaagcaaa gatcgtagat ttctgagtcc tcatttcgtc agcagtccac
     3421 agccaaatgc actgggtcat tcttagaatt ctccttccag tttcgggact gctgtcttga
     3481 gaatgtatct acccttgcca tagttgccct cgggccttat caccagggcc aggtagtatc
     3541 cgggattgat ctggcgcatg ataataccgc acctgtctga gataatggaa aactcgcgcg
     3601 caacgccaag gccaagttct tgtgcagcaa gattaatgtc tttgatcatg gctgaggcct
     3661 ctgctccaag gtcctcaagg ctgaaaagtt tctcggtggc atactcctca accgggatac
     3721 catcagagcc gagaatcatg gcagaaactg caccatcaac cctctccact gcgtccttaa
     3781 gaacctcaga aaagctcatc cctccccgat tcctgcaatt accttattgg ctctttccaa
     3841 aatttctcct tactccgtcc agaaatgtct ccagtttggc tatcaccacc tcgtcacccc
     3901 tgcccatgag tttcatgagc gtcttcaact ccaaaatcct ctgcaaggtc tgcttatcat
     3961 caggattccg tgagaggacc tccctgtaga tctcaagcgc cttgaaaaaa ttaccgctgg
     4021 caaccataga atctgcagcg cctatatcag cgccgcttcc cgcaggtttt gcctcttccc
     4081 ccatcctggg ggctgaaaga tccggatgtt cctggggaac ctcaaccggc tcggcaagga
     4141 tatcatcaaa ggcctctccc tctaaaaggt caattgcctg atcttcagga atctcaatgg
     4201 gctcatcgat ccccggcatg gtctccgggg tctcgacaga cagagaatct tctaacttct
     4261 caaactcctc atgggatatc gcctggggtt caactacagt ctcctcttca tgagtatgcg
     4321 tctcaatggc aggggcttca atttcctcaa catcaggaag cggctcaaca gggctctcca
     4381 agggaaagac aatctcctct tt
//
LOCUS       DATKOU010000216         5589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4152055, whole genome shotgun sequence.
ACCESSION   DATKOU010000216 DATKOU010000000
VERSION     DATKOU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152055"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VMU21_10115"
     CDS             complement(<1..274)
                     /locus_tag="VMU21_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUO77917.1"
                     /translation="MAIDHLNMAGRKITKELTEEEKKGMPLYSIGVVSELLGTTDQTL
                     RLYEKHGLITPARRNKNRFYSENDIKWLRCVRDLIHVKKISIEGIKK"
     gene            356..1345
                     /gene="rfaE1"
                     /locus_tag="VMU21_10120"
     CDS             356..1345
                     /gene="rfaE1"
                     /locus_tag="VMU21_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HUO77918.1"
                     /translation="MVGYGVTMNLKGIVKNFRNSKILVIGDLILDRYIWGKVRRISPE
                     APVPVVEVTDDNFMLGGSANVANNIIALGGEATVAGVAGKDRAGDVMRQLLDEKGIES
                     ALFEDTRPTTIKTRVIAHNQQLVRFDKEESKRISGKALENLVAFVQHAAAEHTAVIVS
                     DYKKGVVSRELIRGILKAPRQKRKFLALDPKVGHFHYYKGVSLITPNLMEASQGSGVE
                     IKDEPSLIKAGKILMKRLACGAVLITRGEEGMSLFEKDRIIHIPTVARHVYDVTGAGD
                     TVIAAFTLAYASGATLREAAIIANHAAGIVVGEVGTAVVTPEKLLESLREIRK"
     gene            1528..2643
                     /locus_tag="VMU21_10125"
     CDS             1528..2643
                     /locus_tag="VMU21_10125"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HUO77919.1"
                     /translation="MPKAIALLSGGLDSTLAILVMLRQGIEVTAVTFLNHFGCDISDR
                     SSCSRNPFPAAEQFGFRVKLCHLSSQFLDIVKNPKFGHGKNMNPCIDCRILMLREAKE
                     LMDLTGADFVVTGEVIGQRPMSQKKNTLAMIDKEAGVKGYVLRPLSAKLMTATIPEEK
                     GLVRREMLYDFSGRSRKPQMALARELGLTDYPAPAGGCLLTEPNYSYRLRELLSHTPD
                     PSLNDLHLLRLGRHFRFSPRCKAIVGRDERENGALESFGDKESYLLWVEDAGSPTVLV
                     SGEGAGDFIPLAASLCAGYSDAKHLPEVEVTVVKGRNPLSVKSNELEKNTHHGSQVTD
                     HMLRIRVSPAESELIEAYRIEKPNIKGRNALSVLHDE"
     gene            complement(2684..3523)
                     /gene="hisF"
                     /locus_tag="VMU21_10130"
     CDS             complement(2684..3523)
                     /gene="hisF"
                     /locus_tag="VMU21_10130"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HUO77920.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVENAKFYDEQGA
                     DELIFLDITASHEKRKIILHVVEKTADDVFMPLTVGGGIKNLDDIRDLLRAGCDKVSI
                     NTSAVKDPFFISRAAERFGSQCIVVAIDAKRVAPDLSGATGEEWFDNPSLKDVCLGPH
                     THALAWAISTHGGRTMKLIDAVRWARKMEALGAGEIMLTSMDRDGTKDGYDIELTKAV
                     SEAVTIPVIASGGAGTLEHLYDGLVHGKADAVLAASIFHYREFTVREAKEYLRAKGVL
                     VRL"
     gene            complement(3615..4340)
                     /gene="hisA"
                     /locus_tag="VMU21_10135"
     CDS             complement(3615..4340)
                     /gene="hisA"
                     /locus_tag="VMU21_10135"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HUO77921.1"
                     /translation="MLIIPAIDLKDGKCVRLLQGREDAATVYSEDPVLTARLWESCGA
                     RLLHVVDLDGAFTGSQKNLQAVKRIRESVGMEMEMGGGIRDMEKIDELVGMGIHRVIL
                     GTVAIEKPALLKEACKKYPGKIIAGIDAREGKVAVKGWVEVTTIDAKSLARESERKGV
                     VGIIYTDIARDGMMTGPNIMAVSEIVRTVNIPVIASGGVSSLEDIKRLRDIEGLWGVI
                     TGKAIYSGSLDLREAINLVKSNV"
     gene            4461..5150
                     /locus_tag="VMU21_10140"
     CDS             4461..5150
                     /locus_tag="VMU21_10140"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HUO77922.1"
                     /translation="MHFLSAEALPFAVPETLNYDLTWTGIKAGEASLEIRDEGSEMKI
                     ISTARSAKWVSMFYTVNDVVESMLSKKNPSTGQPVNYKINLREGRNRKNKEVIFDYRN
                     LKALSIDYLDKERAEVEIPARVFDSLSSFYYVRTLNLEVGRSVYVTVFDNKKVWNIEV
                     QVLRKEKIEVPAGEFNTIVIKPLMKSEGVFYRKGDIFIWLTDNEKRVPVMLKTKVKIG
                     FITARLVGGMY"
     gene            5147..>5589
                     /locus_tag="VMU21_10145"
     CDS             5147..>5589
                     /locus_tag="VMU21_10145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO77923.1"
                     /translation="MIPVSVVIVTKDEERNIKDALESVREFEEVVVLDAFSADATVEI
                     CRRYTPRVFQQEWMGYARQKQAAVDLAKKEWVLILDADERVTPQLKRELAGKIGEGIR
                     DGFYLPRKNFFLGKWIRHSGWWPDYTLRLFRKEVSHMEYREVHEKV"
BASE COUNT         1378 a         1361 c         1473 g         1377 t
ORIGIN      
        1 gcttcttgat gccctcaatg cttatctttt tcacatggat gagatctctc acacacctca
       61 gccatttgat atcattctct gagtagaatc tgtttttgtt gcggcgggca ggggtgatga
      121 ggccatgttt ttcgtagagc cgcagggttt ggtccgtggt gcccagcagt tccgagacaa
      181 ccccgatgga atagaggggc atgccctttt tctcttcctc ggtaagttcc ttggtaattt
      241 ttctgccagc catattcagg tgatcgatag ccataattat aactcatttg ttaattgaaa
      301 aatcaagtac aatattccgg gagtgcagaa gggcaattca ttgatttttg aggctatggt
      361 aggttatggt gtaacgatga accttaaggg aatagtgaag aacttcagaa acagcaagat
      421 tctggtcatt ggtgacctca tccttgaccg ttacatatgg ggaaaggtga ggaggatctc
      481 ccctgaggcc cctgttcctg tggttgaagt cacagacgat aattttatgc ttggtggctc
      541 tgccaatgtg gcaaacaaca ttattgccct tggaggggag gctactgtgg cgggggtggc
      601 gggtaaagac agggcagggg atgtcatgag acagctcctt gatgaaaagg ggattgagtc
      661 cgcgctcttc gaagacacga gaccgaccac gataaagacg agggtcatcg cccataatca
      721 gcagctcgta cggttcgaca aagaagagag caagaggatt tcgggaaagg ccctggaaaa
      781 tctcgtggct tttgtccagc atgccgctgc tgaacatacc gcggttattg tctctgacta
      841 caaaaagggt gtggtctccc gtgaactgat caggggaatc ctgaaggccc ccaggcagaa
      901 acgaaaattc cttgcgcttg acccgaaggt cggacatttt cactattaca aaggggtcag
      961 ccttattacc cccaacctta tggaggcttc ccagggttcc ggagtggaga taaaggatga
     1021 gccgtcactc atcaaggcag gcaagatcct tatgaagcgt ctcgcctgtg gcgcagtact
     1081 catcacaagg ggggaagagg ggatgagcct tttcgaaaaa gaccggatca tccatattcc
     1141 cacagtggca agacatgtgt atgacgtcac gggtgcaggg gacactgtga ttgcagcctt
     1201 tacccttgcc tatgcgtcgg gagccactct gcgggaggcg gccattattg ccaatcatgc
     1261 ggcagggatt gtggtgggag aggtgggtac cgcggtagtc acccctgaga aactcctgga
     1321 gtcactgagg gaaatacgta agtagtaaat agtgagaagt gaactgtaaa gtgtatagag
     1381 taaggcacaa taagaagtgg ccttcgcgca agaggaattt cggccaggtc gaaatggcgg
     1441 gaagaaatgg ccatccggtg agaccgtttt atgtttcatc gcttttgcca ttcactattt
     1501 actgctcact attaactgtc ctctgatatg ccaaaggcga tcgcattact ttcgggcggg
     1561 cttgacagta ccctcgccat cctcgtgatg ctgagacagg ggatagaggt tactgcggtc
     1621 acctttctca accactttgg gtgtgacatc agtgaccgtt cctcctgttc cagaaacccc
     1681 tttcctgctg cggagcaatt cgggtttcgt gtgaaactct gccacctgtc atctcaattc
     1741 ctcgatatag tcaagaaccc caagttcggg cacggcaaga atatgaaccc ctgcattgac
     1801 tgcaggattc tcatgttgag agaagcgaag gaattgatgg acctgacagg tgcggatttt
     1861 gtcgtgacag gggaagtgat cggccagagg cccatgagcc agaaaaagaa cacccttgcc
     1921 atgattgata aggaggctgg ggtaaaaggg tatgtgctga ggcccctcag tgcaaagctc
     1981 atgacagcga ccattcctga ggagaaggga ttagtgaggc gggagatgct ctatgacttc
     2041 agtggccgat caaggaagcc gcagatggcc cttgcgaggg aattgggact cactgactat
     2101 ccggcgcctg cgggaggatg tctcctcaca gagcccaact attcataccg cctcagagaa
     2161 ctcctcagcc atactcctga cccgtcgctc aacgacctcc acctgctgag gctgggaagg
     2221 cacttcagat tttctccgcg gtgcaaggcc attgtgggaa gggacgagag ggaaaatggc
     2281 gctctggagt cttttggtga taaggagagc tatctcttat gggtggaaga tgcaggaagt
     2341 cccacagtcc ttgtctcggg tgaaggagcg ggagatttta tcccgcttgc ggcgtcactc
     2401 tgcgcaggct attctgatgc aaagcacctt cctgaggtgg aggtgacggt tgtcaaaggc
     2461 cgcaatccgt taagcgtaaa gagtaatgag ttggaaaaga atacccatca cggatcacaa
     2521 gtcacagatc acatgttgag gataagggtt tctcctgcgg agagcgaact cattgaagca
     2581 tacaggatag aaaagccgaa catcaaaggc cgtaacgcgt taagcgtact acatgacgag
     2641 taaaggatta aacgcgttac ggttcatgca tcacgtgtca tagttacagc cttacaagga
     2701 ctccctttgc cctcaggtat tcctttgcct ctcttacggt aaactccctg tagtggaaaa
     2761 tggacgcagc aaggactgcg tccgccttgc catgcacaag tccgtcatag aggtgctcga
     2821 gggtccccgc acctcctgat gcaataaccg ggatggttac cgcctctgac actgcctttg
     2881 tcaattctat gtcatagccg tccttggtcc catccctgtc catactggtg agcatgattt
     2941 cccctgcgcc cagggcttcc attttccttg cccatcgaac tgcgtcaatg agcttcatgg
     3001 tccttccgcc atgggttgag atggcccagg cgagggcatg ggtgtgcgga ccgaggcaga
     3061 catctttgag cgacgggttg tcaaaccact cttccccagt tgccccggac aggtcagggg
     3121 caaccctctt tgcatcaatg gcaaccacaa tgcactggct tccgaatctc tctgcagccc
     3181 tgctgatgaa gaaggggtcc tttaccgccg aagtgttgat ggagacctta tcgcatcctg
     3241 ccctgaggag gtcccgtata tcgtcaagat ttttgattcc ccctcccacg gtcaagggca
     3301 tgaacacatc atcagcggtc ttttcgacca catggaggat tatcttcctc ttttcatggg
     3361 acgcggtgat gtcgagaaag atgagttcat cagccccctg ctcgtcgtag aattttgcgt
     3421 tctcgacagg gtcccctgca tcccgtaaat ttacgaaact gacccctttt accacccgtc
     3481 cgtccttcac gtcgaggcat gggataatcc tcttagccag catctttgaa ccccgcgata
     3541 agtgaatcgt ggattgtgaa aaataaaaac acagttctta aagatgtctt ttcgctcatt
     3601 cctcttgtta ctccttacac gttactcttt acgaggttaa tggcttctct caggtcaagg
     3661 cttcccgagt atatggcctt accggtgatc actccccaga ggccttctat atcccggagt
     3721 ctcttaatgt cctcaaggga agagactccg ccggaagcga ttacagggat attcaccgtt
     3781 cttactatct cactcaccgc catgatattc ggtcctgtca tcatgccatc tcttgcaata
     3841 tcagtataga ttattccgac cactcccttt ctttctgact ccctcgcaag agactttgcg
     3901 tcaatcgtgg taacctctac ccatcccttt accgcgacct ttccttccct tgcatcgata
     3961 cccgcgataa tcttcccggg gtatttttta caagcctctt tcaggagtgc aggtttttca
     4021 atggcaacgg tcccgagtat gaccctgtga attcccattc ccaccaactc atctatcttt
     4081 tccatatccc tgatgccccc gcccatctcc atctccatgc ctacggactc acgtatcctc
     4141 ttcaccgcct gcaggttctt ctggcttcct gtgaatgcgc cgtcaaggtc aactacatgg
     4201 agtaaccttg ccccgcagga ctcccacagt ctcgcggtga ggacaggatc ctcagaatag
     4261 acagtggctg catcttccct gccctggagg agtctgacgc atttgccgtc ctttaaatct
     4321 attgcaggaa ttatgagcat gtattaatat aacaaaatat gttcccggta tgcaggagca
     4381 gccatgatgg agagagcaga catagaaccc ccagacatag taagagcctg attttcactc
     4441 ttatctcagt tcctctcctt ttgcattttc tctctgctga agccctcccg ttcgccgttc
     4501 cggaaaccct caactatgac cttacatgga cggggatcaa ggcaggagaa gcatccctgg
     4561 agataaggga tgagggaagt gagatgaaga taatttccac tgcaaggtcg gcaaaatggg
     4621 tctctatgtt ctatacagtg aatgatgtgg tggaaagcat gctttcgaaa aaaaacccct
     4681 cgacgggcca gccggtgaat tataaaatca acctgagaga gggaaggaac aggaagaata
     4741 aagaggtgat attcgattac cggaacctga aggccctttc catcgactat ctcgacaagg
     4801 agcgagcaga ggtcgaaatc ccggcccgtg tgtttgattc cctctcaagc ttctattatg
     4861 tgcggacact gaatctggag gtgggcaggt cggtctatgt cacggtcttt gacaataaga
     4921 aggtgtggaa catcgaggtt caggtgttga ggaaggagaa gatagaggtg ccggcgggag
     4981 agtttaatac gattgtgatt aaacccttaa tgaaatcaga aggggttttt taccggaagg
     5041 gggatatatt catctggctc actgacaatg aaaagcgggt cccggtgatg ctcaagacaa
     5101 aggtgaagat aggcttcatc accgcccggc ttgtgggagg aatgtattga tccctgtttc
     5161 tgtggtgata gtcaccaagg acgaggagag gaatatcaag gacgcccttg aaagtgtgag
     5221 ggagtttgaa gaggtcgtgg tcctggacgc cttcagtgca gatgccaccg tggagatatg
     5281 caggagatat acgccaaggg tgttccagca ggagtggatg ggctatgcac gacaaaaaca
     5341 ggcagcagta gatcttgcga aaaaggaatg ggttctgatc cttgatgcag atgaacgggt
     5401 cacccctcaa ctgaagaggg agttggccgg gaaaatagga gaaggtatac gggatggctt
     5461 ttatctgccc aggaaaaact tcttcctggg taaatggata cgccacagcg gatggtggcc
     5521 tgattacacc ctgaggctct tcaggaagga agtatcccat atggagtacc gggaagttca
     5581 tgaaaaagt
//
LOCUS       DATKOU010000217         8362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4917323, whole genome shotgun sequence.
ACCESSION   DATKOU010000217 DATKOU010000000
VERSION     DATKOU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4917323"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1529
                     /locus_tag="VMU21_10150"
     CDS             327..1529
                     /locus_tag="VMU21_10150"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HUO77924.1"
                     /translation="MAKKIVLAYSGGLDTSVAIKWLKETYGAEIIAFCADLGQGEDLN
                     EVKEKALKTGASKVYVEDLREEFVSHYIFPMLRANAVYEGFYLLGTSIARPLIAKKQI
                     EIAKKEGADTVSHGATGKGNDQVRFELTYYALKPDIKVIAPWREWPFKSRQSLIEYSE
                     KHKIPVTATKEKPYSTDRNLFHISYEGGILEDPWAEAPSDMYTMMVPPEKAPDKPAYI
                     ELSFKAGDSFALNGKPMSPALLLETLNSIAGSNGIGRVDIVENRYVGMKSRGVYETPG
                     GTLLHVAHRAIESLTLDREVLHLRDSLMPKYAELVYYGYWISPEREAIQLMVDEIQKD
                     VTGEVRLKLYKGNCLAVGRKSEYSLYHQELATFEEEEIYNQKDAEGFIKLNALRLRIR
                     NLLRNSHD"
     gene            1522..2643
                     /gene="dprA"
                     /locus_tag="VMU21_10155"
     CDS             1522..2643
                     /gene="dprA"
                     /locus_tag="VMU21_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HUO77925.1"
                     /translation="MTNLESWIALSMVPEIGNVTFKKLLSVYGAPEEVFNAPLRTLSR
                     TEGVGEKKAHQIKSFSRWKDVEKQLKLLIQHNARLVTYHSQEYPAMLRQTEDAPVSLY
                     VRGEIKDEDRFAIALVGSRKATPYGKAMAERLSSALAESGFTIVSGMARGIDTIAHTG
                     SLTSGGRTIAVLGSGLDKFYPPENRGLMERITSSGYVVSEFSFGTMPNRENFPRRNRV
                     ISGLSFGVVVVEAASGSGALITASSALEQNREVFAVPGNVTSSNSTGTNELIKKGAKL
                     IQKADDVIEELAPMLKGFVRADKIKGNQGGTAFPRTPVELSDEDRRVCDILTGEPTHI
                     DVLSRELSLSPAKTLSLLLNLELKGIVKQAEGKRFYLMY"
     gene            2701..2880
                     /locus_tag="VMU21_10160"
     CDS             2701..2880
                     /locus_tag="VMU21_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77926.1"
                     /translation="MANRFIKQSEEKDGSLYLDDEELDAIRSLLICILMKNTRLDKDE
                     IYTYIFGEGKTILWH"
     gene            2892..5198
                     /gene="topA"
                     /locus_tag="VMU21_10165"
     CDS             2892..5198
                     /gene="topA"
                     /locus_tag="VMU21_10165"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HUO77927.1"
                     /translation="MKSLVIVESPAKANTINKILGKNFSVKASIGHIKDLPKKEMGVE
                     VEKGFQPHYVTIPGKEKVIRELKKAAKEADRVFLAPDPDREGEAIAWHIASVITDAKS
                     KGGDQKIFRVTFNEITERAVNEAIKNPGKIDLNKVDAQQARRILDRLVGYGLSPLLWK
                     KVRRGLSAGRVQSVAVKLIVDREREIDAFQKEEYWNINLNLEGSAPPPFMSRLYRYHG
                     SPVINREAEGKERFLIRTSEDAGRIAGEIEKKSLSVSTVEKKERKRSPYPPFTTSTLQ
                     QEGARKLRYTAKKTMMLAQQLYEGVELGEEGSVGLITYMRTDSVRVAPEAQQWARDYI
                     GKRFGKEYLPERPPAYKSKGTAQEAHEAIRPTDMRRPPDTIKQFLPKDLFNLYTLIWN
                     RFIASQMCPARLEQTTLIVMDAEKHLEVRASGTVVKFDGFMALYTESKDEIEEEEGGI
                     LPPLKAGDSLKLIEIKPSQHFTQPPPRYTEATLVKTLEEKGIGRPSTYAAILSTIQER
                     KYVHKGEGRFGPTELGVVVNDLLVDKFPELIDIGFTAKMEDELDSIEEGTMKWVEAVK
                     DFYKPFSHALSEATKTLGKIRPADIPTDIMCDKCGQPMVIKWGRHGRFMACSGYPTCK
                     NTRPLEGQEPKEVVTTDEKCEICGSPMVLKSGRFGKFLACSRYPECKTTKPLSTGVNC
                     PEDGGNIVERRTKKGKPFWSCSNYPKCTFATWNKPVPRQCPKCGAPFVFEKKDKTGQT
                     ILFCHKKECGYKETMMEKPVADEAVPIG"
     gene            5282..5977
                     /locus_tag="VMU21_10170"
     CDS             5282..5977
                     /locus_tag="VMU21_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77928.1"
                     /translation="MTRPILIGIGGAHSGSGKTTYASLLFQRLKGWGGIKYTKTDIYC
                     SLVDERDMLMTEGKDTRRMLDAGAERVLWVKSPPSELGDLLPLAMEKLSDLKGVIVEG
                     NSAIEFLVPDIIIFTFGNDSENLKESAKGILAKADVVLSEEAAPGVVPGETKWFKKSS
                     IDNEKFLISITEMVEMKDKIRASLNERSENGRIPCPVARTIAEELKVDYQEVGRTANE
                     LGIKITGCELGCF"
     gene            5977..6792
                     /gene="ccsB"
                     /locus_tag="VMU21_10175"
     CDS             5977..6792
                     /gene="ccsB"
                     /locus_tag="VMU21_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HUO77929.1"
                     /translation="MSVLFFELSLTFYFAATIVSVVELLKGTKTTSQFILVFAVGGFI
                     LHTATIITRYVVAGHLPITSLHEASSFFAWCIVLIFFFLEYRYRVDLLGPFTMPVVFV
                     LMLSSSILPREIKPLSPVLQSYWLGIHTFLAFLGDAAFATAFGIGIMYLIQEHHLKTK
                     RLGGLFQRLPNLQILDEINYKLITLGFPLLTLAIITGALWAESAWGSYWRWDPKEVWS
                     LITWFIYALVLHARLTAGWRGRKAAMLSIVGFISVIFTFFGVNLLLKGLHAFV"
     gene            6789..8066
                     /gene="hemA"
                     /locus_tag="VMU21_10180"
     CDS             6789..8066
                     /gene="hemA"
                     /locus_tag="VMU21_10180"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HUO77930.1"
                     /translation="MNILVIGLNHKTADVEVREKLAFDGQKLEEGLTMLSTLPGIEEA
                     IILSTCNRVEIYSKVKEPGTAFETVKDFLSRFHGLDTAALERSLYLHRNQDAVRHVFR
                     VASSLDSMVVGEPQILGQLKGAFEFALGKKTTGILLNRLMKKTISVAKRVRTETRIAE
                     NAVSISFAAFELARKIFTDLPEKSFMLLGAGEMAELAAKHMINFGVKKVTVANRTYER
                     GCDLARELNGTAVKFEDFLHEAVHTDIIICSTGAPTYVLHKEEMQRVMKERKQKPVFI
                     IDISVPRNIDPGINDLDNVYLYDVDDLQGVVETNILERKKEAEKAEDIIAEEIESFQK
                     WMLSLDSVPTVVALRSKAETIKKEELERLLNRFPSLGEKERKAIEHMASSITNKLIHP
                     PTVALREDTEDKDTLIATIKKLYGINGDDDEKK"
     gene            8063..>8362
                     /locus_tag="VMU21_10185"
     CDS             8063..>8362
                     /locus_tag="VMU21_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HUO77931.1"
                     /translation="MNKKRIVIGTRGSKLALWQAEWVKSELEKLTPGVKVELNKIKTT
                     GDKILDVPLAKVGGKGLFVKEIEEALLSGAADLAVHSMKDVPTDFPDGLHLPVICK"
BASE COUNT         2307 a         1959 c         2264 g         1832 t
ORIGIN      
        1 aaaaacggct atcagaacac aggatataac tggctaataa taaaattaaa agcaggttat
       61 gttacgcata gctgggtgta ctcctcaaaa aagctatgcg ttgtatcgga tgaaacaaac
      121 gacgcctcgc ggtgccattg atggtgcttt atttatcctg catcactggc aggttccgcc
      181 ggaatatgca tagggcgaag aaaacgatcc gccatttctg aatttcatga tcattcagaa
      241 aacccttttc cttccttttt gaaatttgtt tttcatttat gctatggtgt actaatttag
      301 cgaggcagca agcggaggtt tcaattatgg caaagaaaat tgtgctggcg tattcgggag
      361 ggcttgatac ctctgtcgcg ataaagtggt tgaaggagac ctatggagca gagatcatcg
      421 ccttctgtgc ggaccttggc cagggtgaag acctcaacga ggtgaaggaa aaggcgctca
      481 agacaggggc atcaaaggtc tatgtggaag acctgaggga ggaattcgtc tcccactaca
      541 tcttccccat gctcagagca aatgcggtat acgagggctt ctaccttctc ggcacatcca
      601 ttgcaaggcc tctcatcgcg aagaagcaga tagagattgc aaagaaggag ggtgctgata
      661 cggtctccca tggggcaaca gggaagggca acgatcaggt gaggttcgag ctcacgtact
      721 atgcgttaaa acctgatatc aaggtaattg ctccctggag ggaatggccc ttcaagtcac
      781 gccagtcctt aatagagtat tccgagaaac ataaaatccc tgtcaccgcc acaaaggaaa
      841 aaccgtatag cactgacagg aacctcttcc atataagtta tgaaggaggg attctcgaag
      901 atccatgggc tgaagcgcct tcggacatgt acacgatgat ggtaccccct gagaaggccc
      961 ctgataaacc tgcctatatc gaactttcct ttaaggcagg ggattccttt gcactcaacg
     1021 ggaaacccat gtcccctgcc cttcttttgg aaaccctgaa ttcgattgcg ggaagcaatg
     1081 gtatcgggag ggttgatatc gtggaaaaca gatatgtggg gatgaagtcc agaggggtat
     1141 atgaaacccc cggggggact ctcctccacg tagcccaccg ggcaatagag tcccttaccc
     1201 tggacaggga ggtcctccac ctgagggatt ccctcatgcc aaagtatgca gagcttgtgt
     1261 actacgggta ctggatatcc ccggaaaggg aggcgattca gcttatggtg gatgagatac
     1321 agaaggatgt caccggagaa gtgagactta agctctacaa aggaaactgt cttgcagtgg
     1381 gaaggaagtc ggaatattca ttgtatcatc aggagctcgc cacattcgaa gaagaggaaa
     1441 tctataacca gaaggatgca gagggtttca taaaactcaa tgcccttcgt ctgaggatca
     1501 gaaacctgct cagaaatagt catgactaat cttgaatcat ggattgccct ctctatggtc
     1561 ccggaaatag ggaatgtcac tttcaaaaag cttctctccg tatatggagc acctgaggag
     1621 gtcttcaacg ctccccttag aactctctcc cgcactgagg gagtggggga aaaaaaggcc
     1681 caccagatta agagtttttc ccgctggaag gacgtggaaa agcagttgaa acttctcatt
     1741 cagcataatg cgcgtctggt aacgtatcac agtcaggaat atcccgcgat gctccggcag
     1801 acagaagatg cgccggtcag cctttatgtc aggggggaga tcaaggatga agacaggttt
     1861 gccatcgccc ttgtgggctc aagaaaagca acgccctatg gaaaggctat ggcagagagg
     1921 ctgtcatccg cattggcgga atctggattc accattgtga gcggaatggc gaggggcatt
     1981 gacacgatcg cgcatacagg ctcattaacg tctggaggaa ggaccatcgc ggtcctcggc
     2041 tcaggactgg acaaattcta cccgcctgaa aacaggggct tgatggagag gatcacctcc
     2101 tcagggtatg tggtgagcga attctccttc ggcacaatgc ccaacagaga aaacttcccc
     2161 aggagaaaca gggtgataag cggactttcc tttggtgtgg tggtagtgga ggcagcctct
     2221 ggcagtggag cgctgattac tgcgagctca gccctggagc agaaccggga ggtcttcgcc
     2281 gtgcccggga atgtcacatc ctcgaactcg accggcacga atgaactcat taaaaagggg
     2341 gcaaagctca tccagaaggc ggatgatgtc atagaggaac tggcgcccat gctcaaggga
     2401 tttgtgagag cggacaagat caaggggaac caggggggca ctgcgtttcc ccgaacaccg
     2461 gtggaactct ccgatgaaga caggagagtc tgtgatatac tgacaggaga accgacgcac
     2521 attgatgtgc tctccaggga gctttccctc tctccggcca aaacactttc ccttcttctg
     2581 aatctggaac tgaaaggaat tgtgaaacag gctgaaggca agagattcta tcttatgtac
     2641 tgaacagcac ttgcaaataa cagaaggttt gtggaagaat gaattcttcc gcgtgtaggt
     2701 atggcaaaca gattcataaa acaatcagag gaaaaagacg ggtcacttta tctggatgat
     2761 gaggaacttg atgccatacg gtcattactc atatgcatcc tcatgaagaa tactcgtctt
     2821 gacaaggacg aaatctacac ctatattttt ggtgaaggaa agacaattct ctggcactga
     2881 aggaggctat cgtgaaatcc ctcgtaatcg tggaatcgcc tgcaaaggca aatacgataa
     2941 ataagatact gggcaagaac ttctcggtga aggcgtccat aggccatata aaagacctgc
     3001 ccaagaagga gatgggcgtt gaggtggaga aggggttcca gccccactac gtgacaatcc
     3061 ccggcaagga aaaggtcatc agggaattga aaaaggcggc aaaggaggct gacagagttt
     3121 tcctcgcccc ggaccctgac cgcgaaggag aggccatcgc atggcatatc gcatccgtaa
     3181 ttaccgatgc aaaatctaaa gggggagatc aaaagatctt cagggtcacc ttcaatgaga
     3241 tcactgagcg ggcggtgaat gaagcaataa agaatccagg gaagatagac ctcaacaagg
     3301 tagatgccca gcaggcgaga aggatattgg acaggcttgt ggggtatgga ctaagtccgc
     3361 tcctctggaa aaaggtacgg cgaggactga gcgctggaag ggttcagtca gttgcggtaa
     3421 aactcattgt ggacagggaa agggagattg acgcctttca gaaagaggag tactggaata
     3481 tcaatctcaa tctggagggt tcagcgccac cgccgtttat gtccaggctt taccgatacc
     3541 atggttcgcc ggtcatcaat cgcgaggcag agggaaagga acgctttctc atcaggacat
     3601 ctgaggatgc aggacggatt gcgggagaga tagagaaaaa gagcctttcg gtctcaactg
     3661 tcgagaagaa agagagaaag agatcgccct accctccctt caccaccagc acccttcagc
     3721 aggaaggtgc cagaaaactg agatataccg caaaaaagac catgatgctg gcgcagcagc
     3781 tctatgaggg tgttgaactg ggtgaagaag ggtcggttgg cctcatcacc tacatgcgca
     3841 cagactcagt gcgtgttgcg cccgaggcgc agcaatgggc acgggattac atagggaaaa
     3901 ggtttggaaa ggaatatctg cctgaaagac ctcctgcata caaaagcaag ggtactgcgc
     3961 aagaggccca tgaggcaatc aggcctacgg acatgcgcag acccccagat accataaaac
     4021 aatttcttcc aaaggatctt tttaacctgt atacacttat ctggaaccgg ttcattgcga
     4081 gccagatgtg ccctgcaagg cttgaacaga caactctgat cgttatggat gccgaaaagc
     4141 atctggaggt gagggcttcc ggaactgtag tcaagtttga cgggtttatg gctctgtaca
     4201 cggagagcaa ggatgagatt gaggaagagg aaggaggaat actgccgcca ttaaaggcag
     4261 gagactcttt gaagctcatc gagataaaac cctctcagca ttttacccag ccgcctcccc
     4321 gttataccga ggctaccctt gtgaagaccc tggaggaaaa ggggattgga agaccgagca
     4381 cctatgcagc cattctctcc accattcagg agaggaagta cgtacacaag ggagaaggcc
     4441 gcttcggacc cacagaactg ggcgtggtgg tgaacgacct ccttgtggat aaatttcctg
     4501 agctcattga catagggttt accgcaaaga tggaagatga actggacagc atagaagagg
     4561 ggacaatgaa gtgggtcgag gcggtaaagg atttctacaa gcctttcagt catgcccttt
     4621 ccgaagccac aaaaacctta ggaaagataa ggcctgcaga tatccccacc gacattatgt
     4681 gtgacaaatg cgggcagccc atggtaatca agtgggggcg tcacgggaga ttcatggcgt
     4741 gttccggtta ccccacctgc aagaacacac gaccgctgga gggtcaggag ccaaaggagg
     4801 ttgtcacgac agacgagaag tgtgagatct gtggctcccc catggttctc aagtcaggcc
     4861 gcttcgggaa attccttgca tgttcccgat atcctgaatg caagaccaca aagcccctct
     4921 ccaccggggt gaactgtccc gaggacggag gcaatattgt ggaaagacgc acaaaaaagg
     4981 ggaagccttt ctggagctgc agtaattatc cgaaatgcac atttgccacc tggaataaac
     5041 ccgtcccccg gcaatgtcct aagtgcggcg ctccctttgt tttcgaaaaa aaggataaga
     5101 caggccagac gatccttttc tgccacaaga aggagtgcgg atataaggag actatgatgg
     5161 agaaacctgt agcggacgag gctgttccca taggataagg ggtgagggag agaattcctt
     5221 aaccttggcc tcagcctgaa tttattcccc cttctttagt tttctgaatg tatcttttcg
     5281 tatgacgaga cccatactca taggaatcgg aggagcgcac agtggttccg gaaagaccac
     5341 ctacgcatct ttgctctttc agagactcaa gggatggggt ggaatcaaat acaccaagac
     5401 cgacatatac tgctctctcg ttgatgaacg tgacatgctt atgaccgagg gaaaggacac
     5461 caggagaatg ctggacgccg gcgctgagcg ggtactatgg gttaagtcac cgccttccga
     5521 actgggagac ttgctccccc tggcaatgga gaaactctcg gaccttaaag gggttatcgt
     5581 agagggtaac agcgccattg agtttttggt gcctgatatt ataatattta catttgggaa
     5641 tgattctgaa aacttaaagg aaagtgcgaa ggggattctc gcgaaggccg atgtagtgct
     5701 ctctgaagag gcggcacccg gagtggtacc gggagagaca aagtggttta agaaatcgtc
     5761 tatagacaat gagaaattcc tgatctctat aacagaaatg gtcgagatga aagacaagat
     5821 acgggcgtca ctcaacgagc gttccgaaaa tgggagaatc ccctgccctg ttgccaggac
     5881 aatcgccgaa gaactgaagg tggattacca ggaagtgggc aggaccgcca acgagctagg
     5941 cataaaaatc accggctgtg aactcggttg cttctgatga gcgtgctctt ctttgaactc
     6001 agcctcacct tctattttgc tgccaccatc gtgagcgtgg tagaactcct taaagggaca
     6061 aaaaccacat ctcaattcat ccttgtgttt gcggtgggcg gtttcatcct gcataccgcg
     6121 actattatca ccagatacgt ggttgccgga catctcccca tcacaagtct ccatgaggca
     6181 tcgtctttct ttgcatggtg tattgtgctc atcttctttt tccttgaata ccggtaccgg
     6241 gttgacctcc tcggtccctt caccatgccc gtggtattcg tgctcatgct ctcttcttcc
     6301 atcctcccca gggagataaa acccctgagc ccggtgctcc agagctactg gctggggatc
     6361 cacaccttcc tcgccttcct cggtgacgcc gcctttgcaa cggcctttgg aattggcatt
     6421 atgtacctca ttcaggagca ccacctaaag acaaaacgcc tggggggctt gtttcagaga
     6481 cttcccaatc tccagatact ggatgaaata aactataaac tcattaccct gggatttccc
     6541 ttattgaccc tggctatcat caccggtgcg ctgtgggcag agagtgcatg gggaagctac
     6601 tggagatggg accccaagga ggtatggtca ctcatcacct ggtttattta cgcccttgtc
     6661 ctccatgcgc ggcttactgc aggatggcgg gggagaaagg cggccatgct ttccattgtg
     6721 gggttcataa gtgtaatctt cacctttttt ggcgtaaatc tccttcttaa agggttgcat
     6781 gcgttcgtat gaatatcctt gttattggcc tgaatcataa aacggcggat gtggaggtga
     6841 gggaaaagct cgcctttgac ggccagaaac tggaagaggg cctgaccatg ctcagcacgc
     6901 tccccggcat tgaagaggcc atcatcctct ccacctgtaa cagggtagag atctattcca
     6961 aagtgaaaga acccgggacc gcgttcgaga ccgtaaagga ctttctctcc cgctttcacg
     7021 gattggacac tgctgccctt gaacggtcac tctacctcca tagaaaccag gacgcggtgc
     7081 gccatgtatt cagggttgcg tccagccttg attccatggt ggtgggcgaa ccccagattt
     7141 tggggcagct gaagggcgca tttgagtttg ccctggggaa aaagacgacg ggcattctcc
     7201 tgaacaggct gatgaaaaag accatatcgg ttgccaagag ggtgaggacc gagacaagga
     7261 tcgcagaaaa tgcggtctcc ataagtttcg ctgcgtttga gctcgcgaga aagatattca
     7321 ctgacctccc ggaaaagtcc tttatgctcc tgggtgcggg tgaaatggca gaactcgcgg
     7381 caaaacatat gattaatttc ggcgtcaaga aggtaaccgt tgcaaacaga acctatgagc
     7441 gcggctgcga tctcgcaaga gagctgaacg gaacggctgt gaagttcgaa gattttctcc
     7501 acgaggcagt gcataccgat atcatcatct gctccaccgg tgctcccacc tatgtgctcc
     7561 acaaagagga aatgcagcgg gtgatgaagg agagaaaaca gaagccggta ttcattattg
     7621 atatctccgt accccgtaat attgacccgg ggataaacga cctggacaac gtctatctct
     7681 atgatgtgga tgacctgcag ggggttgtgg aaacgaacat cctcgaaagg aaaaaggaag
     7741 cggaaaaggc ggaggatatc atcgcagaag agattgaatc attccagaaa tggatgttat
     7801 cccttgactc tgtgcccacg gtagttgccc tcaggagcaa ggcagagacc atcaagaagg
     7861 aagagctgga acgtcttttg aatagatttc cttccttagg agaaaaggag cgcaaggcca
     7921 ttgagcacat ggcctcgtcc attacgaaca agctcatcca tccccccaca gtggctctca
     7981 gggaagatac cgaggataag gataccctca ttgcgacaat caaaaaactc tacgggataa
     8041 acggagacga cgacgagaaa aaatgaataa aaaaagaatt gtgatcggaa cccgcgggag
     8101 caaactcgcc ctgtggcagg cggaatgggt aaaatcagag ctggagaaac tcaccccggg
     8161 cgtgaaagtg gaattaaaca agatcaagac taccggggac aagatattgg atgtccccct
     8221 tgccaaggtg ggaggtaaag gccttttcgt aaaagagatc gaggaagcgc tcttgtccgg
     8281 ggccgctgac cttgcagtgc acagcatgaa ggatgtgcct actgactttc ctgacggcct
     8341 ccatctgccg gtcatctgta ag
//
LOCUS       DATKOU010000218        29992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1084113, whole genome shotgun sequence.
ACCESSION   DATKOU010000218 DATKOU010000000
VERSION     DATKOU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 29992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29992
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084113"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="hemC"
                     /locus_tag="VMU21_10190"
     CDS             <1..679
                     /gene="hemC"
                     /locus_tag="VMU21_10190"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HUO77932.1"
                     /translation="EDPRDAFISRMQNSEFRIQGFKKLPNAATVGTSSLRRSCQLLSM
                     RPDLKIAQLRGNLDTRLRKLDEGQFDAIILAAAGVKRLGWSHRITEILSTAISLPAIG
                     QGAIGIECRIGDPFINDLIAPLNHPETSTCVKAERALLKKLEGGCQVPIAAHARLVEG
                     RIVMDGLVGSVSGDRIIKGHIQGSTEHSESLGITLAEDILSRGAKEILDEVYERFSAP
                     VDGEISS"
     gene            636..1097
                     /locus_tag="VMU21_10195"
     CDS             636..1097
                     /locus_tag="VMU21_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HUO77933.1"
                     /translation="MNDSLPLLTAKSAVKRIFTLGTDRRSEEDFIEILLSYNIAAIID
                     VRSVPKSRIPTFTRDNLEGLLKREGLEYFFLGRELGGFRKGGYPAYLVTEEFTHGIET
                     LESIAEKRQAVIICAERFPWKCHRKWIARELHQRGWNIEHIIDKGKVWVPK"
     gene            1135..1476
                     /locus_tag="VMU21_10200"
     CDS             1135..1476
                     /locus_tag="VMU21_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77934.1"
                     /translation="MEKKSFLTAVTEVMKGMISHETMMASLQRSCMRGEAENLDLMRQ
                     KETEVDRLIEGMSSIGELNIPEDSMDVLVLRYLRNTQFLACIKEEAIDVSELGNNTLN
                     YWKKTMLYKTP"
     gene            1599..3644
                     /gene="ligA"
                     /locus_tag="VMU21_10205"
     CDS             1599..3644
                     /gene="ligA"
                     /locus_tag="VMU21_10205"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HUO77935.1"
                     /translation="MSEKIPAEIKHEIEKLVKDLNYHNYRYYVLDAPVISDAEYDRLF
                     LRLKELEATSHHVLPDSPTLRVGAPPLEKFAKVKHKEPMLSLDNAFSYDEVKEFDRRI
                     GRLLNTEKEIEYTVEPKYDGLAIELTYREGLLHRASTRGDGYEGEDVTQNIKTIKSIP
                     LRIEGLEIPGEIDIRGEVYMDLDEFEKLNKQREQKGEPPFANPRNAAAGSVRQLDPSI
                     TSSRKLYLACYGIGTVRGIVFKNHSEFIRWLEESRFPTPAMVKLVKKIEGVIDAIREI
                     ERKRNSLPFESDGAVIKVNDFGLQQRLGVKTREPRWAIAYKFQAHQGTTRIKEILGSV
                     GRTGVITPFAVFEPVRIGGVTVSRSTLHNWDEIERKDIRVGDTVVVERAGDVIPHVVM
                     VVKEKRTGKEKPPPIPDKCPACGAKVVREEDEVAVRCVSLHCPAQVQEKIIHFASRGG
                     MDIEGLGEKNVELLYSKGLIKHFEDIYRLTKEDLIELPRFAEKSAQNLIDAIKKSKNA
                     TLAKFLFAIGILHVGEYAAKLLAKNFERIEDLYHVKPDRLMGIRQMGEKISSSISAFF
                     NDQKNIETLKTLKAFGLKLTNPGFSSEKKGERPFEGLTFVITGTLPKSRKEVEDLIEG
                     NGGHSSSSVSASTDYLVAGEDPGSKLEKAKTLGIKTISYDELLTLIKRKTENPGLFD"
     gene            complement(3694..4452)
                     /locus_tag="VMU21_10210"
     CDS             complement(3694..4452)
                     /locus_tag="VMU21_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO77936.1"
                     /translation="MSVLIGLLAGILGGLLGMGGGIVMIPLMVGFFKVTQHRAHGTSL
                     AAMVFIGLVGAATYALQGSVDLLASVLLATTAILTVYRGARHASSLPEWKLRRSFGAF
                     VVLVSILLFLKPFIPPFVYFSEGWKKVFILLAAGSFTGFLSGMLGVGGAVIMIPVMVL
                     LVGLDQHTAQGSSLLTMVPAGIVGAWTHMRYGNIETRLLRGLIPGVFIGTYVGGTLAH
                     FFQDGVLRFIFGIMLLWTGYQYLKTPVSKQVLQA"
     gene            4677..5333
                     /locus_tag="VMU21_10215"
     CDS             4677..5333
                     /locus_tag="VMU21_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUO77937.1"
                     /translation="MPIQLVIFDLDGTLIDSRIDITNALNYALAPYPFAKLSVEDTVK
                     MIGEGITRLIEKLVGKRDTSMKADVTKRFLTYYTQHILDYTKEYPGVRETIEKLNSYQ
                     KAVISNKKETLSRKVLDGLGLLHYFDVIIGSDTTPERKPSPMPILKVLSELHVRPEEA
                     VIVGDSNYDVDAGKAAGISTVAVTYGYRPREVIAHADYLIDRMTDLVPLLRRMEELQG
                     "
     gene            5330..6055
                     /locus_tag="VMU21_10220"
     CDS             5330..6055
                     /locus_tag="VMU21_10220"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HUO77938.1"
                     /translation="MKEITLDSIGDIRLYQSRTGYRFSVDALLLYSFVNVPKAKKIAD
                     LGAGSGVVGLLLAKKYPDAKVSLFELQERLSTLAKKNIALNSLEGRAKVINADIREMK
                     PCSTASGMDCFDIAVSNPPFRKPKTGLISPEEERAIARHEIRLKLPELIRAVHHLLCS
                     KGRFFLIHHPERLTELAGSLKERGMEVKRLRFVHSHMWSVAKMVLVEAVKSGKGGVKV
                     ERPLYIYNEHGGHSEEMIRIYGG"
     gene            6141..6923
                     /locus_tag="VMU21_10225"
     CDS             6141..6923
                     /locus_tag="VMU21_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HUO77939.1"
                     /translation="MIRNTFSILNGIGEKLERRLWMDGILTWDDFIRTNAIRFISPER
                     KPFFDWSLYTASQKLGERDSTFFAKTIRRREHWRLFEIFRGNALYLDIETNGLQPGRG
                     GYVTVVGLYDGYDWKSLVSGENLTADNLRKELSGYKCLVTFYGSSFDIPFLQRTFPDV
                     KFDMPHFDLCFAAKRVGIHGGLKKIETMFAIERNEDVKGMDGYDAVRLWELAKRGSSE
                     AKRLLLSYNKEDTVNLVGIADILYHRLKQSTGIEEYLTCGVT"
     gene            6934..7812
                     /gene="xerD"
                     /locus_tag="VMU21_10230"
                     /pseudo
     CDS             6934..7812
                     /gene="xerD"
                     /locus_tag="VMU21_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
     gene            7906..10401
                     /locus_tag="VMU21_10235"
     CDS             7906..10401
                     /locus_tag="VMU21_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO77940.1"
                     /translation="MKSLGVDELFRCCDEKALNFETTDELPSLEGTIGQARALSAIDF
                     GLSLESSGFNIFLLGENGTGRMSTIKTILKEQSLTKPVPSDWCYVYNFKDPDAALAIS
                     LAPGQAVIFQRDMEELVKTLRVEIPKVFESKEYEKQRSKTMEEFQKKQRDLFSSLEEE
                     AQAKGFSIRKTVSGLLIVPVKKTGEPLTEEEFEGLDADTKKKIEELGKLLQEKLDDVV
                     RAVREGEKLLKDLLLKLEREAALSSVGHLIEDIEKKYRENEKITHYLEDVKEDILAHL
                     EDFKSSEEQPAPLPFMKLPKTEPTFTRYTVNVLVNNREAKGAPCVFESNPAYYNLFGR
                     VEHKFQYGMAVTDFSMIKAGSLHRANGGYIVINVLDLLRNLFAYDALKRAIRNREIKI
                     EDVWEQYRLVSTTSLKPEAIPLDVKVILVGSPYLYYMMYSLDEEYRELFKVKADFDSR
                     MERTDENIQKYALFIATLCKQENLPPFDRSGVTKIVEFGSRLSEHQEKLSSRFSDIAD
                     LIRESTHWAKVAESVVVKAEHVEKAVSEKVYRNNRIQERLQELMQEGTLIVDTSGAKK
                     GQVNGLAVLDLGDYSFGKASRITATTYTGKAGVVNIERETKMSGKIHEKAIMILTHYL
                     GSKYAIKKPLSLSASITFEQLYDMVEGDSATCAEFYALTSSISGVPLAQNVAVTGSMD
                     QTGVVQPIGGVNEKIEGFFELCKLRGLDGSHGVIIPRKNVKNLMLKKDVVDAVREGKF
                     SIYPIEAVEEGLEILTGMSMGQLGEDGTYPEGTFNYLVMKRLTEIFEALKEKKDEKEE
                     PEKKESSGSQEKQKTTKSGKSRKTPKGIFNRKR"
     gene            10398..11279
                     /locus_tag="VMU21_10240"
     CDS             10398..11279
                     /locus_tag="VMU21_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUO77941.1"
                     /translation="MRRLWWGLQAAVFLMCSLPLSFLPVKAGELFGLILCYLWKGRRE
                     IATDNLKKSVALHALSLPYSPEEMIREHFKNLGRSFIEIIKIYYGRGKGILRETTIKG
                     LENFEQAKAKGKGVIFLTGHCGNWELLAIAFSFHVTSSSLVARRLNNPYLNTLLERAR
                     SRFGNRAVYKQGALRAIISTLRSGGNVAILMDQAVLAEEGFVIDFLGRGAWTSKMPAL
                     IARKTAAAVLPVFIRRNGKGHEITIHPEVSLSSLSDYEHAVREDTQRFSRFIEDYIRT
                     NPSQWLWLHRRWKRVPQ"
     gene            complement(11597..12418)
                     /locus_tag="VMU21_10245"
     CDS             complement(11597..12418)
                     /locus_tag="VMU21_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HUO77942.1"
                     /translation="MSAITISRGSYRRGKEIAEKVAHTLGYECISREILLEASQQFNI
                     PEIKLEKAIHDAPSILERFTHGKEKYIAFIRATLLKELQKDNVVYHGLAGHFFLQGVP
                     HVLKVRITADIEDRVREEMKITGFSSPSLTRDLLKKDDEERRKWGLRLYGKDTWNPDL
                     YDMVLHVQTMSVDEATQIIVTASKLPSYQTTPRSQSILDDLCLAAQVEASLVHEFNVA
                     HVTAKEGSIHVAIRASAKEEAAIVQKVTEIAQHVFGVKAVSVDVVEAAFLLGMGR"
     regulatory      complement(12595..12670)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(13283..14119)
                     /gene="rfbD"
                     /locus_tag="VMU21_10250"
     CDS             complement(13283..14119)
                     /gene="rfbD"
                     /locus_tag="VMU21_10250"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HUO77943.1"
                     /translation="MKILVAGSKGMLAHDLIPVLGERHEVIAPQEAEFDITQRASSYE
                     FAKKTSPHVIINCAAYTNVDRAEQERDKAFLVNGTGVQNLALICHEMNIPLCHISTDY
                     VFDGTKSLPYTPFDGTNPINTYGESKLAGEKYIQWIMNEFYIVRTSWLYGKHGNNFVS
                     TILRLAGERKDLRVVDDQRGSPTSTVTLSLALRALLENGAYGVYHFTDETKGGISWFD
                     FAKEIIRISGHHTEVIPIPTRDFPRPAKRPVNSVLDTAVFSTVTGHSPVDWKDALKEY
                     LR"
     gene            complement(14116..15123)
                     /gene="rfbB"
                     /locus_tag="VMU21_10255"
     CDS             complement(14116..15123)
                     /gene="rfbB"
                     /locus_tag="VMU21_10255"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HUO77944.1"
                     /translation="MRLLVTGGCGFIGSSFIRHILSKYPGYRIVNFDALTYAGNPENL
                     KDIQGDARYVFVKGKIEDAALVSDLVQDADCVIHFAAESHVDRSILDAQPFLTTNVMG
                     TYVMLTASRDAGTKRFIHISTDEVYGTLGDEGKFTEDIPLRPNSPYSASKASGDMLVR
                     AYHETYGLPAIIVRPSNNYGPYQYPEKFIPLMITNLLENKTIPVYGEGRNVRDWLFVG
                     DTCSAIDLILHKGRDGEVYNVGGNAERRNIDIARKVLEMMGKDERAITYVANRPGHDY
                     RYALDNSKIERELGWKPSMDIEKRLEATIRWYRDNEWWWKPLKERLSSESKGFWETKK
                     T"
     gene            complement(15123..15581)
                     /locus_tag="VMU21_10260"
     CDS             complement(15123..15581)
                     /locus_tag="VMU21_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HUO77945.1"
                     /translation="MFRDGDIHGVQVRKLKEFHDSRGWLSELYRMDELGDYTPAMGYV
                     SLTRPYFTRGPHEHIEQTDYFCFLGSFSLYLWDNRKGSSTYGNRMVIENADRFIVVVP
                     PGIVHAYRNSGEHEGMVLNFPDRLYAGGGRKEKVDEVRHEDDPGSPFQVA"
     gene            complement(15582..16649)
                     /locus_tag="VMU21_10265"
     CDS             complement(15582..16649)
                     /locus_tag="VMU21_10265"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HUO77946.1"
                     /translation="MKALILSGGKGTRLRPLTYTTAKQLVPVANKPILGYVIDHIRDA
                     GVKDVGVIISPETGDEVRRYIDGGRKWGIHVTYIPQSEPHGLAHAVSTARDYLKEDSF
                     IMYLGDNLLSHGVKDALKKFRHERPQALIFLKEVDNPKQFGVAKLDRNGTILKLIEKP
                     KRPPSNLALVGVYIFSREIHDAISRIKPSFRGELEITDAIQELINMGHEVRSEILTGW
                     WLDTGKKDDLLQANTVVLDEYIKREVNGSVDSRSQITGRVTIERGAVIKNSIIRGPVI
                     IGKETEVTGSFIGPFTSIGEGVIITNSVIEHSVILNNALLSGIERLEDSLVGKNTKVI
                     RKSTLHKALRLMVGDDSIIEV"
     gene            16766..17758
                     /locus_tag="VMU21_10270"
     CDS             16766..17758
                     /locus_tag="VMU21_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUO77947.1"
                     /translation="MKTILVTGCAGFIGSVVSQKLLHSGYAVLGVDNMNDYYDPRLKE
                     WRLKGLSGNPKFRFIEIDIADYQGLRTALEPEKVTYHLGNPVDAVINLAARAGVRASV
                     EDPWAYLDTNTKGTLNLLECCKEFGIRKFVLASTSSIYGLNEMPFRETDNTDRPLAPY
                     SATKKAAEALCYTYHFLHGLDISIPRYFTVYGPAGRPDMSIFRFIRNIDTGKSIPVFG
                     DGKQKRDFTYIDDIAEGTLKCLQPFGYEFFNLGNDHPSELMQVINLIEGHLGKKAVID
                     WFERHPADVPATCADIEKSRRLLHWEPAVGIEEGIRNTVQWYREHREFLLTIKG"
     gene            complement(17755..18405)
                     /locus_tag="VMU21_10275"
     CDS             complement(17755..18405)
                     /locus_tag="VMU21_10275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUO77948.1"
                     /translation="MISLALNKDKIDDALGKAVDILKKGGIIAYPTETFYALGVKFDL
                     PRSLKRLYELKKRPREKAMPVIIGRRTALSGIVSPEWLERIPPFAQALMNRFWPGPLT
                     LLFPAQEGLPEDLTAGTGIIAVRMPGESFALPLTQHAGFPVTATSANLSGTPPADSAE
                     MVIEYFGERVDLVIDGGQTPGGLPSTIVDVSGGRVTVVREGVIMRDEIVRFASQVS"
     gene            18495..19115
                     /locus_tag="VMU21_10280"
     CDS             18495..19115
                     /locus_tag="VMU21_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO77949.1"
                     /translation="MWKERPFAGRGGTMSEERISCEISEEDLRAALREIKTYVDITEE
                     DLKKIYAIALRNAQERTARSIPVSDVMTKDTIAIRTHADIHEAAGLLSEHRISGLPVV
                     DEEGVVLGVVTEADILSMTGMKEGHTFRDVLRHILGEPLPERKRGNSVGEIMNSPAIT
                     TRPDADVRDVARILDEKRIKRLPVVDERNRLVGVISRADIVRAMRK"
     gene            19112..19654
                     /locus_tag="VMU21_10285"
     CDS             19112..19654
                     /locus_tag="VMU21_10285"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HUO77950.1"
                     /translation="MIREYLSKMKGGAKSPPGVGITEVIWSWLGAALGIGICAYLSSS
                     YFEPLDLTLLIGSFGASAVLVYAAIKSPLAQPRNLVGGHVISGLTGVACFNLFGGTLW
                     IAAAMSVSLAIAMMLITKTLHPPGGATALIAVIGGEKIHNLGFLYAFVPAGLGAIVLL
                     IVALLVNNISKNRRYPEYWF"
     gene            19891..21237
                     /locus_tag="VMU21_10290"
     CDS             19891..21237
                     /locus_tag="VMU21_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HUO77951.1"
                     /translation="MKRRILESSVLFISISKWIFLASCVGVMVGFSTTVFLKLLGLGI
                     ASASGFKYYFLLLPFAFFASVILVKYLAPEAEGHGTEKVIEAVHKRAGKINPMVVPVK
                     LAATIITIALGGSAGKEGPCAQIGAGLSSGLADLLKFNDKDRRKLVICGISAGFATVF
                     GTPIAGAIFGVEVLFVGGLLYDVLLPSFVAGIVGFQVSSSLGITYFHEPLNFVPVFSS
                     FFFIKVCVGGIFLGLCSFLFVEMLKYFKELSGKLKMPMQYKGLLGGSTLALLTLLLST
                     RYLGLGLNSIQTSLTGGEIPKPAFFLKMLFTSITLNFGGSGGIVTPIFFVGATAGNMF
                     GSVFGLDPAVFAAIGMVSLLAGAANTPISASIMAIELFGASIGPYAAVSCVISFLMTG
                     HRSVYPSQVLSMRKSSSIAVEKGRVMEDIGKIDVQIRTKSVVGQIAKALKKMRKIT"
     gene            21323..22759
                     /gene="pyk"
                     /locus_tag="VMU21_10295"
     CDS             21323..22759
                     /gene="pyk"
                     /locus_tag="VMU21_10295"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HUO77952.1"
                     /translation="MKLPPHKTKIVCTIGPASESPAMMEEMIIAGMGVARLNFSHGVL
                     EDHGRVIQNLRLAARRTGQRLAIMADLPGPKMRIGQLAREPITLKPGGSFTLTSEEIV
                     GDSNRVSVTFSRLPQVVKAGNTLFLNDGLIQLEVVKVEGNNVICRVVVGGELRSRKGL
                     NLPGIDLGISAFTDRDFQCIKFALEHGVDIISQSFVQSGADVSAVRRSATSLGYSPFI
                     ISKIERSIAFEHIEGILNETDGIMIARGDLGVEIPIEKIALAQKWLMRKANMAGKPVI
                     TATQMLESMIGNPRPTRAEATDVANAILDGTDCVMLSEESAMGRYPLEATSMLARIAA
                     TIEPYHPAHHVREALRRASGQEETIGVKDLIAHSVETAVERISPAAIFVPTHTGATAR
                     NVTRYRLPVWIIAVSSQENTCQCLQLSYGVYPQFEAVHPENWNSYVKDWMSAHELEGN
                     MIVLIEGPSSRHPEANNRMEIIQLDRVV"
     gene            22935..23297
                     /locus_tag="VMU21_10300"
     CDS             22935..23297
                     /locus_tag="VMU21_10300"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77953.1"
                     /translation="MLNRRQCKRFPEHCNTEFASDGITYHAISRDFALSGLFIVTGSP
                     RAPGTLLQIKIRLPDGSTALLLGKVMRFYKTFGGNDVAPVQQAEDGMGIEILEKDLNY
                     LRFIRSLLETPGKHEPDN"
     gene            23316..23642
                     /locus_tag="VMU21_10305"
     CDS             23316..23642
                     /locus_tag="VMU21_10305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO77954.1"
                     /translation="MRDKRQSTRFEKRCETDFLAEGITHRGICLDLSLNGLFIETDFR
                     FSPDTMLNILIHLPDGTISKLKGKVVRCLDNGIGVGIIEKDASYLHYYSRCLLEPEAH
                     APLREP"
     gene            23937..24587
                     /locus_tag="VMU21_10310"
     CDS             23937..24587
                     /locus_tag="VMU21_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HUO77955.1"
                     /translation="MKAMIIESLNIGLPKKERFHGREMLTGICKIPVSGPLLLNKLGF
                     DGDGVGDMKHHGGPDKAVCVYSTDHYPYWQEIMGMKLPVAAFGENLSVANLREDDICI
                     GDIFELGTALVQVSQPRQPCRTLAARYARDDMVRLVVDSGRTGFYLRVLKEGQVEKDS
                     TLILKERDPRRITIAFANQTYHHDNKNCEAIKRILKVPYLSESWKRSFHELAKRCR"
     gene            complement(24611..24943)
                     /locus_tag="VMU21_10315"
     CDS             complement(24611..24943)
                     /locus_tag="VMU21_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77956.1"
                     /translation="MVLDVVVNNRSVSIQIEDPFHPDLDAIMGQIDRAASSHAIDISG
                     FDIRGLIPRMIEGIAGCERGCPANAKGFVARGFKDFTLQYIEGGILSAHASMGDGKGV
                     ALKMFPDF"
     gene            complement(24971..26773)
                     /gene="aspS"
                     /locus_tag="VMU21_10320"
     CDS             complement(24971..26773)
                     /gene="aspS"
                     /locus_tag="VMU21_10320"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HUO77957.1"
                     /translation="MMRDKGCGELRESDIGSVMSLAGWVFRRRDHGGLIFVDLRDRSG
                     LVQVVFSPDVSADAHTQGGDLRAEFVIHVSGEVRRRPEGTVNKNLPTGMVELYANSLT
                     VLNESSPVPFPMEEAAEASEFLRLKYRYLDLRRPELQQNLIIRHKATKVMRDYLDERG
                     FVEVETPMLTKSTPEGARDYLVPSRLNPGQFYALPQSPQLFKQILMVSGLEKYFQIVK
                     CFRDEDLRADRQPEFTQVDLEMSFVEREDIIHLIEGMMKRLFMDVLKVDISVPFERLS
                     FRESMERFGNDKPDLRFSLELKEMADLAKNGSFKVFLDAMASGGRVKAINGKGMAGFS
                     RKEIDTLTEEAKAFGAKGLAWIKVKDGFESPIAKFFPEEILRQMAQRLDSEEGDLMLF
                     VADKDKVVHDVLSRMRLEFGKNLNLMQPVFKFAWILDFPLLEWDEDEGRFQAMHHPFT
                     SPAEDDLETLRSLDLSSPEMYTAQRSQLAAFKAKAYDIVLNGYEIGGGSIRIHRRDVQ
                     KKMFEVLGIGDEDARVKFGFLLDALEFGAPPHGGIALGLDRLVMLMVGASSIRDVIAF
                     PKTQKAVCLMSGAPSGVEQKQLRELSIKTVDAGS"
     gene            complement(26810..27538)
                     /locus_tag="VMU21_10325"
     CDS             complement(26810..27538)
                     /locus_tag="VMU21_10325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HUO77958.1"
                     /translation="MEKRIQKILSELGIASRRKAEELILEGRVTVNGQIATIGMKADP
                     SRDYIKVDGKRVTGSGKEMQKTYLMFHKPRGVMTTLSDPEGRPTVKDFLKGVKYRVFP
                     VGRLDYASEGLLLLTNDGDFSNAILHPSQKIPKTYLVKVKGVMEEENIQKLRQGVKLD
                     DGVTMPARVKKIRQVENNSWIEITIHEGRNRQVRRMIEKVGHLVLKLKRTGVNGLKLG
                     DLKPGEMRLLTPDELRLIKKEIGI"
     gene            27657..28424
                     /locus_tag="VMU21_10330"
     CDS             27657..28424
                     /locus_tag="VMU21_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUO77959.1"
                     /translation="MKREKETKILKTAKPKTASWKDNISFILVEPREPGNIGASARAM
                     KNMGFTHLELVKPRSFMTEETKKMACNAFDILARAEIYGSLNEALEAKTLVVGTTRRL
                     GRTRGLILHLEEGIKKVIASAKKNRVAILFGREDKGLNNREVEECGFLITIPSDPSSP
                     SLNLAQSVLLVAHELSRKTYSGQFPRAVTRPELEILHHHIHDTLKLLEYIPRGNRDLE
                     ARIMRNLKHLLARAGLTDWELNMLHGICGQIEKKMGQ"
     gene            28482..29135
                     /locus_tag="VMU21_10335"
     CDS             28482..29135
                     /locus_tag="VMU21_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HUO77960.1"
                     /translation="MIKTLVCIDRDGTLIYDEKEHLYLGRDNDWKAKVKILPYVIEGM
                     RALKAIPDAAVYMITNQPGVAITDFPLLTLERAHEVCRYILEKIKDIGGRIDGYFLCP
                     HANQAYVKKYTQYHFDKRLVCECSCIKPDLGMIFDALRSEGVTPGSVSIYMIGDRATD
                     VQTALNMGGTGILVPFENQPGEDQEVNKIMDQSCIYIAKNLLDAAEFIALHTRAGGG"
     gene            29299..29745
                     /locus_tag="VMU21_10340"
     CDS             29299..29745
                     /locus_tag="VMU21_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77961.1"
                     /translation="MKHNRYLSFVIGISFFAMLFLPGIMNNVQAQGKQETSVEGRLGE
                     TPTKEKFSEEKREFKSKTQKRLKEVDKKIRELEAESKKAGSKAKEEAKEGLQDLKKKH
                     AELKKEMRTLEAKSKKEWETVKQKVGAAMDELEEAYNKVRNKFKSE"
BASE COUNT         7839 a         7456 c         7697 g         7000 t
ORIGIN      
        1 agaagaccca cgggacgcct ttatctcgag gatgcagaat tcagaattcc gcatccaggg
       61 cttcaaaaaa ctccccaatg ccgcaacagt agggacaagc agcctcaggc gctcatgcca
      121 gttgctcagc atgaggccag acctgaagat tgcccagctc agggggaatc tcgatacacg
      181 attgcgaaaa ctggacgagg ggcagttcga tgcaatcatc ctggctgctg cgggggtgaa
      241 aaggctcggg tggtctcata ggatcacgga aatcctctcc actgcaataa gtctccccgc
      301 aataggtcag ggggccattg gtatcgaatg caggattggt gatcctttta tcaacgacct
      361 tattgcgcca ttgaatcacc cggagacctc cacatgcgta aaggcggaga gggcccttct
      421 gaaaaaactc gaagggggtt gtcaggttcc cattgctgca cacgcacgac tggttgaggg
      481 aagaattgtg atggacggac ttgtgggaag cgtatcgggc gatcgaatca taaagggtca
      541 tatccagggt agcacagaac attcagaatc cctcggcatt acccttgcag aagatattct
      601 ttccaggggt gcaaaggaga tactggacga ggtatatgaa cgattctctg cccctgttga
      661 cggcgaaatc agcagttaag aggatattta ctctcgggac tgacaggagg agcgaggaag
      721 attttatcga aatactcctg tcctacaata tcgcagccat catagatgta aggagtgtcc
      781 cgaaaagcag gattcccacc tttaccaggg acaatcttga agggctcctg aaaagagagg
      841 ggttggagta ttttttcctc ggcagggaac tgggaggatt caggaagggt ggataccccg
      901 catacctcgt gaccgaggag tttacgcatg ggatcgaaac actggaatcc attgcagaga
      961 agagacaagc ggttatcatc tgcgctgaac gctttccctg gaaatgccac aggaaatgga
     1021 ttgccagaga gctgcatcaa aggggatgga atatcgaaca tatcatagat aagggaaagg
     1081 tgtgggttcc caaatgacag ggtgcatgca aagacccggc tgaaaggaga gcatgtggag
     1141 aagaaaagtt tcctgaccgc agttactgaa gtgatgaagg gtatgatctc ccatgagacg
     1201 atgatggcgt ccctgcagcg ctcctgcatg agaggggaag cggagaacct cgatctgatg
     1261 cgtcagaaag agacagaggt cgacaggctc attgaaggga tgtcatccat aggtgaactt
     1321 aatatcccgg aggattccat ggatgttctt gtcctgagat acctcaggaa cacccagttc
     1381 ctggcctgca ttaaggagga ggcgattgat gtctcggaac tgggaaataa cactctcaat
     1441 tactggaaga agacaatgct ctataagacc ccctagaatt acccttttgt catttcagcc
     1501 acccaaatct ctcacaagcc ttgtccacct ttcaaaatac ttaaaatata gtattattga
     1561 atagcgatgt attattcacc cagacagaaa gaaatagaat gtcagaaaag attcctgcag
     1621 agatcaagca cgagattgag aagctcgtaa aagacctcaa ctatcataat tatcgctatt
     1681 acgttctcga cgcccccgtg atttcggacg cggaatatga cagactcttt ctgcgcttga
     1741 aggagcttga ggcgacctcc caccatgtct tgcccgactc tcccaccctg agagtgggtg
     1801 cccctcccct tgagaagttt gcgaaggtga agcataagga gcccatgctg tctctggata
     1861 atgccttctc atacgatgag gtcaaggagt ttgaccgcag gataggaaga ctccttaaca
     1921 cagaaaagga gatcgaatac accgttgaac ctaaatatga cggacttgcc attgaactga
     1981 cttacaggga aggccttctc cacagggcgt ccacaagagg agatggctat gaaggggaag
     2041 acgtcaccca gaatatcaag accatcaagt ccattcccct gaggatagag ggacttgaga
     2101 tccccggtga gatcgatatc cgaggagagg tctatatgga ccttgatgag ttcgagaaat
     2161 taaacaagca gagggaacaa aaaggcgaac ccccattcgc caatccgcgg aatgccgccg
     2221 cagggtctgt gagacagctg gacccttcca tcacctcatc ccgaaagctc taccttgcgt
     2281 gctacggaat aggaacggtg aggggcatcg tgttcaagaa tcattccgag ttcatcaggt
     2341 ggcttgaaga atccaggttt cccactcccg caatggtgaa acttgtgaaa aagattgagg
     2401 gcgttattga tgccattcga gagatagaac ggaaacggaa ttccctcccc ttcgaatcag
     2461 acggtgccgt gataaaggtg aatgacttcg gccttcagca aaggctggga gtgaaaaccc
     2521 gggagccccg ttgggccatt gcgtacaaat ttcaggccca tcaggggacc acacggataa
     2581 aggaaatcct gggcagcgtc ggcaggaccg gtgtgatcac cccttttgcc gtttttgaac
     2641 ctgtccgtat tggcggcgtc accgtctccc gctcaaccct ccataattgg gatgagatcg
     2701 aaagaaagga tataagagta ggagataccg ttgttgtgga gcgggcaggg gatgtcatac
     2761 cccacgtggt catggtagtg aaagaaaaga ggaccggaaa agaaaagccc ccccccattc
     2821 ccgacaaatg tccggcatgc ggtgcaaagg ttgtccggga agaggacgag gtggcggtca
     2881 ggtgtgtttc ccttcattgc cctgcacagg tccaggaaaa gataatccac tttgcgtcaa
     2941 ggggcggtat ggacatcgag ggactgggcg agaagaatgt ggaactcctc tactcaaagg
     3001 gactcattaa acactttgag gacatttaca gattaacaaa ggaagatctg attgagctgc
     3061 caagattcgc cgaaaaatct gcacagaatc tcatagacgc catcaaaaag agcaagaatg
     3121 ccaccttggc caaattcctc ttcgcgatcg gcattctcca tgtgggcgag tatgctgcaa
     3181 agctccttgc aaagaatttc gagagaatcg aagatttata ccatgtcaaa ccggacaggc
     3241 ttatgggcat acgacagatg ggggaaaaga tatcctcctc catatcagcc tttttcaatg
     3301 accagaaaaa catcgagact ctcaaaacac ttaaggcctt tggcttgaaa ttaaccaacc
     3361 cgggcttttc ttctgagaag aaaggagaaa gaccctttga agggctcacc tttgtcatca
     3421 ccggtacatt acccaagtca cggaaggaag tagaggatct catcgagggc aacgggggcc
     3481 actcgtcttc ttctgtgtca gcaagcaccg attaccttgt ggcaggcgaa gacccgggct
     3541 cgaagttgga gaaggcaaag acactgggaa tcaagaccat ttcctatgac gagttattga
     3601 cccttattaa gcggaagacg gaaaatccgg gattgttcga ttgacctact tttcgcaaaa
     3661 aaaagcagct tctgctgccc tcttcttcac tcatcatgct tgcaagacct gtttcgatac
     3721 cggtgttttc agatactgat agcctgtcca gagcagcata atcccaaaaa taaatctcag
     3781 tactccgtcc tggaagaaat gcgcgagggt tccgccaacg taggtcccga taaaaactcc
     3841 gggaatcaat ccccggagga ggcgggtttc tatattgccg taacgcatat gtgtccacgc
     3901 ccccacaatg cctgcaggaa ccatggtcag gagcgaactg ccctgggcag tgtgctgatc
     3961 gagaccgact aaaagaacca tcacaggaat cattatcact gcgcctccga ctcccagcat
     4021 ccctgaaagg aaacctgtga acgaccctgc cgccagcagt atgaagacct ttttccaccc
     4081 ttcggagaag tacacaaagg gagggataaa tggtttgaga aaaagcagta tcgagacaag
     4141 aacgacaaag gccccaaatg acctcctgag cttccactcc ggtagagaac ttgcgtggcg
     4201 ggcgcctcta tagacagtga gaattgcggt tgttgccaac agtacagagg ccaagaggtc
     4261 caccgacccc tgcagcgcat atgttgctgc ccccacgagt cctataaaga ccatggcagc
     4321 aaggctggtg ccgtgggccc gatgctgcgt aactttaaaa aaaccgacca tgagtgggat
     4381 catgacaatt ccacccccca tacccaggag gccgccaagg atacctgcca aaagtccaat
     4441 cagtacgctc atatttttta cttcagaacc ctcgcacagc gaattattta ttatatacct
     4501 tactgatagg tctgccgctg aaatcagtat cgatcttaca aggttcaaac agtgaatgtg
     4561 aagggcatac gtaacaccac ttgctcggct tcaatctctt taaaacatcg caggaattgg
     4621 acgatgatct ctctctccat ggttgttccc gctgcactag tggtataatt tcgtgtatgc
     4681 ctatccaact tgttatcttc gatctcgatg gcacgctcat tgactcacgc attgatatca
     4741 ccaatgcatt gaattacgcc ctggcacctt accccttcgc gaagctctcg gtggaggaca
     4801 ctgtcaaaat gatcggtgaa gggataaccc gtctcatcga gaaactcgtg ggaaagaggg
     4861 acacctcaat gaaggctgat gtcaccaaga ggttcctcac ctactacacc cagcatatcc
     4921 tcgactatac aaaggaatat cccggggtcc gggagaccat cgagaagctc aattcctatc
     4981 aaaaggctgt gatatccaac aaaaaggaaa ccctttcgag gaaggtactg gatggtcttg
     5041 gactcttaca ctattttgat gtcatcatcg gcagtgatac cacaccggag aggaaacctt
     5101 ctcccatgcc gatactcaag gtgctttccg agcttcatgt gcggccggaa gaggcagtaa
     5161 tcgtgggaga cagcaattac gatgtggatg caggaaaggc agcgggcatc agcacagttg
     5221 cggtcacgta cggatacaga ccaagggagg tcatcgccca tgccgattat ctcatcgaca
     5281 ggatgactga tctggtcccg ctgctccgaa gaatggagga gcttcagggg tgaaggagat
     5341 caccctcgac agtatagggg atataagact ctatcagtcc aggaccgggt accggttttc
     5401 tgtggacgca cttctgctgt attcctttgt gaatgtgcca aaagcaaaaa aaatagcaga
     5461 ccttggtgcg ggttcagggg ttgtgggatt gctcctcgcg aagaagtacc cggatgcgaa
     5521 ggtctccctc tttgaacttc aggagagact ctcaaccctc gcgaagaaga acattgccct
     5581 caactcactt gaggggagag ccaaggtaat aaatgctgac ataagagaga tgaaaccctg
     5641 cagcactgct tccggcatgg actgtttcga cattgcggtc tccaacccac ctttcaggaa
     5701 accgaagaca ggactcataa gccctgaaga ggaaagggcc atcgcacggc atgaaataag
     5761 acttaagctc cctgaactga tacgtgctgt ccatcacctc ctctgctcaa agggcaggtt
     5821 tttcctcatc caccatccag agagactgac agagcttgcc ggttcactga aggaaagggg
     5881 gatggaggtg aagaggctca ggtttgtgca ctcccatatg tggtcagttg ccaaaatggt
     5941 gctggtggaa gcggtgaaaa gcggcaaagg gggagtgaag gtcgaaaggc ccctttatat
     6001 ttataatgaa cacggcggac atagcgagga gatgattcgg atttacggag ggtgataaac
     6061 gggaaatcgt atggattaga ataaagagaa caggtataat acagacaacc aggaaaggcg
     6121 tgtgaccaaa ggtacatatc atgatcagaa acacattcag cattcttaac ggcatagggg
     6181 aaaaactgga aagacggctc tggatggatg gcatcctcac atgggatgat tttatccgca
     6241 cgaatgccat ccggtttata agtcccgagc gaaagccgtt ttttgactgg tcactctaca
     6301 ccgcctccca gaagttgggc gaaagggatt ccaccttttt tgcaaagacc ataaggaggc
     6361 gggaacactg gaggcttttc gagattttca ggggaaacgc cctctacctt gatatagaga
     6421 cgaatggtct ccagcccgga aggggcgggt atgtcacggt ggtgggtctc tatgacgggt
     6481 atgactggaa atccctcgtc tcaggagaaa atctcactgc ggataacctc aggaaggagc
     6541 tttcaggtta taagtgcctt gttacgtttt acggttcatc ctttgatatc ccctttctcc
     6601 agaggacgtt ccctgacgtg aagtttgaca tgccccattt cgacctctgt tttgcggcaa
     6661 aaagagtggg gattcacggc ggactcaaaa agatagagac aatgttcgcc attgaaagaa
     6721 acgaggatgt gaaggggatg gacggatatg atgcggtgag gttatgggaa cttgcgaaga
     6781 ggggcagcag cgaggcaaag cggctcctgc tcagctataa caaagaggac accgtgaatc
     6841 ttgtggggat tgctgatata ctttaccacc gtttgaagca gtccacaggg atagaggagt
     6901 atctgacctg tggagttact tagggatttt ctcacgtacc tttcggtgga gaaggggttg
     6961 tcagagaaca ccgtccgttc ctaccactgt gaccttacca cgttcgggac attccttcag
     7021 ttaaaaggca agacattccc cacgtgttcg aggcccgata tcctcgattt cctagacacc
     7081 ctgaaagaca aagcgtatgc tacgtccagc atttgccgtg ttatctcttc ggtcaagggt
     7141 ctctgtaagt accttctcat agagaaggtc attcccgaag acccgactga aaatctccag
     7201 tcgccaaaga aatgggaaag gctgccgaag gcactgagca tcgaccagat gaaggaagtc
     7261 ctttcctctt catcaaatcc aggggaacag gagaagaagt ccatccatgg agcgttgttc
     7321 ttcaggaact ccgccatgct ggagctcctc tattcatcag gcctccgcgt gagcgaactt
     7381 gtgaagctga agctggcgga tatcagtttt gaagggggat ttgtcaggat catggggaag
     7441 ggttcgaagg aaagggtagt gccggtaaac cggcgaaccc tggaacgggt gaagagctac
     7501 ctgcgcgatt tcagaccatt gcttctcagg aaacgtattt cagaatacct tttcgtgacc
     7561 gggaggggag cgcccatgac acggcaacgc ttctggcaga ccataaaggc ttacgggaaa
     7621 ctgctcggca ttgagctctc tccccacacg gtgaggcaca gttttgccac acatctcctt
     7681 gaagggggcg ctgatctcag gtccctacag aagatgcttg gccactcgga catatccact
     7741 acccagatct ataccaaggt aacgatggac agaataaaaa aggtgtatct cgatcatcat
     7801 ccgagggcgt aggcgcacat atacaagtca acgtgaggac agggaaaaga gaagcacata
     7861 attcttcgtc aagtttttca ttcttgatac ttttggaggt gacccatgaa gtctctcggc
     7921 gtggatgagc tgttcaggtg ttgtgatgaa aaggcattga attttgaaac cacagacgag
     7981 cttccgtctt tggagggaac catcggccag gcgcgggcac tcagtgccat cgattttggc
     8041 ctcagtctgg agagctcggg attcaatatc ttcctcctcg gagaaaacgg cacggggaga
     8101 atgtccacta taaagacaat cctcaaagag caatccctta ccaagccagt cccctcagac
     8161 tggtgctatg tctataactt taaggacccc gacgcggccc tggccatttc ccttgccccg
     8221 ggacaggcgg tgatctttca aagggatatg gaagaactcg taaagaccct gagagttgag
     8281 attcccaagg tattcgagtc aaaggaatac gaaaagcaaa gaagcaaaac aatggaagag
     8341 ttccagaaaa agcagaggga tttattcagc agtctcgagg aagaggcaca ggcaaaaggg
     8401 ttttccatac gcaagaccgt gagtggtctt ctcatcgtgc cggtaaagaa gacgggtgaa
     8461 ccccttaccg aggaggagtt tgaaggcctt gatgccgaca ccaagaaaaa gattgaagag
     8521 cttggcaaac tcctgcagga gaagctcgat gatgtggtga gggcagtgag agagggggag
     8581 aagctcctga aggacctcct gctgaaactg gaaagggaag cagccctctc ctcagtcggt
     8641 cacctcatcg aggatattga gaaaaaatac cgggagaatg aaaagattac gcactatctc
     8701 gaagacgtga aagaggatat cctcgcccat ctcgaagact tcaaatcttc cgaagagcaa
     8761 cctgcgccat tacctttcat gaaactcccg aagacagagc ccacattcac gcggtatacg
     8821 gtgaacgtcc tcgtaaacaa cagggaagcc aagggcgctc cctgcgtgtt cgagagtaac
     8881 cccgcatact acaatctctt cggaagggtt gagcacaaat tccagtacgg catggcagtg
     8941 acagattttt ccatgatcaa ggcaggatcc cttcacaggg caaacggcgg atacattgtc
     9001 atcaatgtcc tggatctact caggaacctc tttgcctatg atgccctcaa gcgggcaatc
     9061 agaaacaggg agataaagat agaggacgtt tgggagcagt accggcttgt ctccaccacc
     9121 tccctaaaac ctgaagcaat acccctcgat gtaaaggtca tccttgtggg aagcccttat
     9181 ctctactata tgatgtacag cctcgatgaa gagtacaggg agctcttcaa ggtaaaggct
     9241 gattttgaca gcaggatgga aaggacggat gagaatattc agaagtatgc cctctttatt
     9301 gccacgttgt gcaagcagga aaaccttccc ccctttgaca gatcaggggt gacaaagatt
     9361 gtcgaattcg gttcaaggct ttcggagcac caggagaagc tctcctccag attcagcgac
     9421 attgctgact taataaggga atccacccac tgggcaaagg ttgcagaaag cgtggtggta
     9481 aaggcagagc atgtggaaaa ggcggtcagt gaaaaggtat acaggaataa ccggatccag
     9541 gagaggctcc aggaattgat gcaggaggga acgctgatcg tggacacctc aggcgcaaag
     9601 aagggacagg tcaacgggct tgcggttttg gatctggggg attacagctt cggaaaggca
     9661 tcaaggatca ctgcaaccac ctacacgggg aaggctgggg tggtaaacat agaacgcgag
     9721 acaaagatga gcggaaagat ccatgaaaag gcgatcatga ttctcaccca ttacctggga
     9781 agcaaatatg ccatcaaaaa gcccctgtct ctctcagcat ccatcacttt tgagcagctc
     9841 tatgatatgg tggagggaga cagcgccacc tgcgccgagt tttatgccct cacgagcagc
     9901 atatccggcg ttccccttgc tcagaatgtc gcggtcaccg gctccatgga ccagaccggt
     9961 gtggttcagc ccattggcgg ggtgaacgag aagatcgaag gcttctttga gctctgtaaa
    10021 ctcagggggc ttgacggcag ccacggggtg ataataccca ggaaaaatgt gaagaacctc
    10081 atgctgaaga aggatgtggt tgatgcggtg agagaaggaa agttttccat ttatcccatt
    10141 gaggcggttg aggaggggct agagatactc accggaatgt ccatgggcca gttaggggaa
    10201 gacggaacct atcccgaggg cacctttaat tatcttgtga tgaagcgcct taccgagatc
    10261 tttgaggccc tcaaagagaa gaaggacgaa aaagaagagc cggaaaagaa ggagagttcc
    10321 gggtcccagg agaagcaaaa gaccacaaag tccgggaaga gccgcaagac gcccaaagga
    10381 atattcaacc ggaaaagatg aggagactct ggtggggact ccaggcagcc gttttcctga
    10441 tgtgctccct gcccctgtct tttctccctg tcaaggcagg agaactcttc gggctgattc
    10501 tctgttacct ctggaaaggg aggagagaaa tcgccaccga caatctgaag aagagtgttg
    10561 cccttcacgc cctctcgctc ccgtattctc ctgaagagat gatcagagag catttcaaga
    10621 atctcggacg ttctttcatc gagataataa agatatatta cggcagggga aaggggattc
    10681 tcagggaaac caccataaag ggtctggaga atttcgaaca ggcaaaggca aagggaaagg
    10741 gagttatttt tctcacaggc cattgcggaa actgggagct attagccatc gctttttctt
    10801 ttcacgtgac atcctcctct ctcgttgcac gtcgattaaa caatccctac ctcaatacgc
    10861 ttcttgagcg ggcgcgctcc cgttttggga acagggccgt gtacaaacag ggtgcattac
    10921 gtgccattat ctcaaccctg cgcagtggcg ggaatgtggc aatcctcatg gaccaggcgg
    10981 tactggccga agagggtttt gtgatcgact tccttgggcg aggtgcatgg acctccaaaa
    11041 tgcctgcgct gatcgcacgt aagaccgcgg ccgcagtgct tccggtattc atcaggagaa
    11101 atggcaaggg tcacgaaatc accatacatc ccgaagtcag tctctcatcc ctcagtgact
    11161 atgaacacgc agtgcgggaa gacacacaaa gattctcccg tttcatagaa gactatatac
    11221 gcaccaaccc ttcccagtgg ctttggttgc acaggagatg gaaaagagtg ccccaataag
    11281 aagtgacaag ggaaagacaa ggcaggagag gggatgaggg ataacaaaaa gtttcttgtg
    11341 gagaacgaag aaaagggcaa agctcactga ggaaagcgca tcgcatgatg cagattctca
    11401 ctgaaaaccg ccctcccttc ggctacggag ttcaccggct gatagttatc ataatttgaa
    11461 acaggtgatg attaccgctt gtatcacgta atgaaggggg ggaagaaaag aagtggcgga
    11521 gagactgccc atccgccaag gtttcaaggt tatttatcct attgagggct acgccttaac
    11581 ctgtcagcag gttttattac cttcccatcc ccaacaagaa ggctgcctcc acgacgtcga
    11641 ctgaaacggc cttaacacca aagacatgct gagcaatttc agtgaccttt tgtacgatcg
    11701 ctgcttcctc tttagctgag gccctgatcg ccacatggat gcttccctcc ttagctgtca
    11761 catgtgccac attaaattcg tgcacgagac tcgcctccac ttgggctgcc aagcacagat
    11821 catcgaggat actctgggat cgcggtgttg tctggtagct gggcaacttc gaagctgtaa
    11881 cgatgatctg ggttgcctca tccacagaca tggtctgaac atgcaagacc atgtcataaa
    11941 gatcggggtt ccacgtgtct tttccgtaca gacggagacc ccatttgcgg cgctcctcat
    12001 cgtctttctt caggaggtcg cgcgtaagcg aaggagaaga gaaccctgtt attttcattt
    12061 cctccctaac cctgtcttcg atgtccgcag taatacgcac cttgagcaca tggggtaccc
    12121 cctggaggaa aaaatggcct gcgagcccat gatatacgac attatctttc tgtaattctt
    12181 ttagcagagt cgcccgtata aaggcgatat atttttcctt gccatgggta aatcgttcta
    12241 aaattgatgg tgcatcatga atcgccttct caagttttat ctctggtata ttgaactgct
    12301 gagaggcttc aagaagtatc tcccgtgaga tgcactcata ccctaaggtg tgagcgacct
    12361 tttccgctat ctctttccca cgtctgtaag aaccccgcga aatagttatg gctgacaaac
    12421 tgttcccact ttctccaaaa ctgattgcat tgttccattc atcggctttg ttaccacatt
    12481 attgtaatcg tctgcacctc ttgtactgaa acaacgaaga tatctcattc acccccatag
    12541 gcagcacccc ctcagatgag aaacaaaaag accaagcata ccaaaggttc gtccttcggc
    12601 agctcggcca tcttaactaa gacccgccgc tttccgtccc cttctcacaa agggtctggt
    12661 cttatcgaga gagcctatat ctaatttctc ttataccata cgcaacaaac aagagctatc
    12721 agtctaagac cgattcttgt gtcggtcttc tccctacata acctgagaaa aggcaaactt
    12781 tgatcgttat ttcggccagc aaccaacact atcatggaag gcctaaaagg tgtttgtttc
    12841 agctgatcaa agttatcttt gcgctcctaa ggggtaaaag gaagtttgat gatggagtgt
    12901 gtgctatcgt aacgcacgca taagaggaca gagaggttgt ggtggaagaa ggacctcagt
    12961 acttcgtagg caataaggcc tgtaggattc agcttggtca acttccacca cccttttcgg
    13021 cctgcataag gtaagttggg aatgaaaggt ccgcagaaca aaagaggtta ggtgaaaagg
    13081 taaaagggtt gaagcaccac cacaaccatc ccagctttaa cagagtaaat agagagatgt
    13141 cttgactctc ttgcttagaa gaggaaggac atctcaccag ttggagggtt caactttaag
    13201 cgagaaagtg ggtcattttt caatgagaat ctacatttgg cgcatacaga actttggggc
    13261 aaacgcgact cacggcagga tctcagcgaa ggtattcttt taacgcgtct ttccagtcca
    13321 caggtgagtg ccccgttact gtggaaaaaa ccgcggtatc caggacagag ttgaccggtc
    13381 tttttgcagg ccgcgggaag tctctggtcg gtatgggaat tacctcggtg tgatgccctg
    13441 aaatccgtat gatttctttt gcgaaatcaa accagcttat tccccctttt gtttcatcgg
    13501 tgaagtgata aacgccatac gcaccgttct ccagaagagc ccttaatgca agggaaagtg
    13561 tcaccgtaga tgtgggagaa cccctctggt catccactac cctcaaatcc ttccgctcac
    13621 ccgcaagtct gaggatcgtg gatacaaagt tattgccatg tttgccataa agccagcttg
    13681 tccttacgat atagaattcg ttcatgatcc actgaatgta tttctcaccg gcaagtttcg
    13741 attcaccata ggtatttatg gggtttgtgc catcaaaggg tgtgtaaggc agggattttg
    13801 tgccatcaaa gacataatca gtgcttatat ggcacaaggg tatattcatt tcatgacaaa
    13861 tcagtgcgag gttctgaacg ccggtgccat ttacgagaaa ggccttatcc ctctcctgtt
    13921 ccgccctgtc aacattggta taggcggcac aattgatgat tacatgggga gaagtttttt
    13981 ttgcaaactc atatgaggag gccctttgtg tgatatcgaa ctctgcctcc tgaggggcga
    14041 tgacctcgtg tctttcccca agaaccggaa tgaggtcatg ggcgagcatg cccttagagc
    14101 ccgcgacaag gatcttcaag ttttttttgt ctcccagaat cccttgctct cagaagacaa
    14161 ccgttccttc agaggcttcc accaccactc attgtcccta taccaccgga tagttgcttc
    14221 cagcctcttt tcgatatcca tggagggttt ccatcccagt tccctttcta tcttggagtt
    14281 gtcgagagcg taccggtagt cgtggcccgg cctgttggcc acataggtga tcgccctttc
    14341 atctttcccc atcatctcca gcaccttcct ggcaatatcg atatttctcc tctccgcatt
    14401 cccgcccaca ttatagacct cgccgtctct gcctttgtgc aggatgagat caatggcgct
    14461 gcaggtatcc cccacaaaga gccagtccct cacattcctg ccctcgccgt ataccgggat
    14521 ggttttattc tcaaggaggt tcgtgatcat aaggggtatg aacttttcag ggtattgata
    14581 agggccgtaa ttattggacg gcctgacgat aattgccgga agtccgtagg tctcgtgata
    14641 ggccctcacg agcatatccc ccgatgcctt tgatgctgag tagggtgaat taggcctcag
    14701 gggtatatcc tcggtaaact tgccctcgtc acccagtgtg ccatagacct catccgtgga
    14761 gatatggatg aacctttttg tcccggcgtc cctcgaagcg gtaagcatta cataggttcc
    14821 catcacatta gtcgtcagga acggctgggc atcaagaatg gacctgtcca cgtggctttc
    14881 cgcggcaaaa tgaatgacgc agtcagcatc ctgcacaagg tcgcttacca gggcagcatc
    14941 ctcgatcttc ccttttacaa aaacatatcg cgcatctcct tgaatgtctt tgaggttttc
    15001 gggattcccc gcataggtga gggcatcaaa gttcacaatc ctgtaaccgg ggtacttact
    15061 aagaatgtgc ctgatgaaac ttgaaccgat gaaaccacac cctccggtca caagcagacg
    15121 catcaggcca cctgaaaggg gcttcctgga tcgtcttcgt gtctcacctc atccaccttt
    15181 tccttacgcc cccctcctgc gtaaagcctg tctggaaaat taaggaccat cccctcatgt
    15241 tccccggaat tcctgtaggc atggacaatc cccggaggca ccaccacgat gaaccggtcc
    15301 gcgttttcta tgaccatcct gttcccgtac gttgatgaac ccttcctgtt gtcccagaga
    15361 tagagagaaa acgagcccaa gaaacagaaa tagtcggtct gttcaatatg ttcatggggc
    15421 cccctggtaa aataggggcg ggtgagcgac acatatccca tggccggcgt ataatcacca
    15481 agttcgtcca ttcgatacag ctcggagagc cagccgcgac tgtcatgaaa ttccttaagc
    15541 tttctcacct ggactccgtg gatatctccg tccctgaaca ttcacacctc tattatggaa
    15601 tcgtccccca ccatgagacg gagggccttg tgaagggtgc tcttcctgat gacctttgtg
    15661 ttcttaccca cgaggctgtc ctcgagtctc tcgatacctg acaagagggc gttattcaat
    15721 atgaccgaat gttctatcac ggaattcgtg ataatcaccc cttcaccgat gcttgtgaac
    15781 ggaccgataa aggaaccggt cacttcggtc tcctttccga tgattaccgg ccctctgatg
    15841 atactgtttt ttatgaccgc tcccctctct atggtaaccc tgccagtgat ctgactcctg
    15901 ctgtccacgg acccatttac ctctcttttt atgtactcat caaggaccac ggtgtttgcc
    15961 tggagaagat catccttctt ccctgtatca agccaccagc cggtgaggat ctcgcttctc
    16021 acctcatgtc ccatattgat taattcctgg atcgcgtccg taatctccaa ttctcccctg
    16081 aaagaaggct ttatccggga aatggcatca tggatctcac gtgagaatat gtatacccct
    16141 acaagggcaa gattcgaggg aggccgcttg ggtttctcaa ttaatttcag gatcgtgccg
    16201 ttccgatcaa gctttgccac accaaactgc ttgggattgt ccacctcttt cagaaaaatt
    16261 aaagcttggg gcctctcgtg cctgaatttc ttcagggcat ccttgacccc gtggctcagg
    16321 agattgtcac cgaggtacat aatgaaagag tcctccttaa gataatccct tgccgtgctc
    16381 actgcatggg caagaccatg gggttcggac tgggggatat aggtcacatg aatgccccat
    16441 ttccttccgc cgtcgatgta tcttctcacc tcatcaccgg tctccgggga aataattacc
    16501 cccacatcct taacccccgc gtccctgatg tgatcaatga catagccgag gataggtttg
    16561 tttgcaactg gcacgagctg ttttgccgtg gtgtaggtga ggggcctcag tctggtccct
    16621 ttcccaccgc ttagtatcag tgctttcatg ttctttcgga cccaaaaaaa ttcgttgtat
    16681 tatagcaaaa atagaaggaa aatcgtagaa tacatgggca tgtgatcgta tcacggctaa
    16741 tgacaatata tttcagaggt gaaagatgaa aacaattctg gtgaccgggt gtgcagggtt
    16801 tatcggctcg gtggtttccc aaaagcttct ccattcaggc tatgctgtac tgggtgtgga
    16861 caacatgaat gattattatg acccgagact gaaggaatgg aggctgaagg ggctttccgg
    16921 caacccgaaa ttccgtttca ttgagataga tatcgctgat tatcaggggc tgagaaccgc
    16981 attggagccg gaaaaggtca cgtatcatct gggaaatcct gtggatgcgg taatcaatct
    17041 tgcggcaagg gctggggtga gggcatctgt ggaagacccg tgggcttacc tggacaccaa
    17101 taccaaggga actcttaacc tcctggagtg ctgcaaagag ttcgggatca ggaaattcgt
    17161 gctggcctca acatcaagta tttacggact caacgagatg cccttccggg agaccgataa
    17221 cacagaccgc cccctcgctc cctattcagc aacaaaaaag gcggcagagg cattatgtta
    17281 tacgtaccat tttctacacg gtctggacat aagcatcccg aggtacttca ctgtctacgg
    17341 tccggcaggg agacccgata tgagcatatt caggttcatc aggaatattg acacagggaa
    17401 gtccatcccc gtgtttggtg acgggaaaca gaagagggac ttcacctaca ttgacgacat
    17461 tgcggagggg accctgaagt gtctgcaacc ctttggctac gagttcttta acctcggcaa
    17521 tgaccaccct tctgaactta tgcaggtgat caatctcatc gaaggacacc tcggcaaaaa
    17581 ggcggtgatc gattggtttg agcgtcatcc tgcagacgtc cctgccacat gtgcagacat
    17641 tgagaaatcc aggagactcc tgcattggga gcccgcagtg ggcattgagg aaggcatcag
    17701 gaacacggtg cagtggtacc gtgaacacag ggaatttctt ctcacaataa aaggctaact
    17761 cacttgtgag gcgaaccgca ctatctcgtc tctcatgata acaccctcac ggactacggt
    17821 aacccttcct cctgatacat ccacgatggt ggaagggagt ccgcctggtg tctgacctcc
    17881 atcaataacc aaatctaccc tctcaccaaa gtattctatg accatctcgg cgctgtccgc
    17941 aggtggcgtc cctgacaggt ttgcacttgt tgcggttacg ggaaatccgg catgttgtgt
    18001 cagtggtaat gcaaaagatt ctcccggcat tcttaccgcg ataatgcctg tgccggctgt
    18061 gagatcctcc ggaagccctt cctgtgcagg gaagagcagc gtaagcggcc ctggccagaa
    18121 tctgttcata agcgcctggg cgaatggagg aatgcgttcg agccactcag gtgagacgat
    18181 cccggaaagg gctgtcctcc ttcctataat cacaggcatg gccttttctc tgggacgttt
    18241 ttttaattca tagagccttt tcagcgagcg gggcaggtca aatttcaccc cgagggcgta
    18301 aaacgtttcg gtgggataag cgatgatccc gccctttttc aggatatcaa ccgccttgcc
    18361 gagagcgtca tcgattttgt ctttgttgag cgccaatgag atcatcgagt tttcccacac
    18421 gtcgcgcagg tcctgattgc aaaggggagc ctgttatgat ataaatcata acagatagcc
    18481 ggcagttaag gctaatgtgg aaagaaaggc catttgccgg gagagggggt acgatgtccg
    18541 aggagcgaat atcctgcgaa atttccgaag aagacctgag ggcagcgctc agggagatca
    18601 agacctatgt ggatatcact gaggaggatc tgaaaaagat atacgccatc gcattgagga
    18661 atgcacagga gaggaccgca cgcagtattc cggtgagcga cgtgatgacg aaggacacca
    18721 tcgcaattcg cacgcatgcg gacatacacg aagctgccgg gctcctttca gaacaccgga
    18781 tcagcggcct gcctgtggtg gatgaagagg gtgtggtgtt gggagtggtc acagaggccg
    18841 atatcctttc catgacaggg atgaaggagg gacatacctt cagggacgtc ctgcgccaca
    18901 tactgggaga accgcttccc gagcgaaaaa ggggaaacag cgtgggagag attatgaact
    18961 ctcctgccat aaccacaaga ccggacgcgg acgtaaggga tgtggcaaga atactggatg
    19021 aaaaaaggat caaaagactc cctgtggttg atgagaggaa cagacttgtc ggggtcatct
    19081 caagggcaga tattgtcagg gccatgagaa aatgatcaga gagtatctct caaaaatgaa
    19141 gggcggcgca aagagcccgc caggggtcgg aataacagag gtgatatggt catggctcgg
    19201 tgcggccctt gggataggta tctgcgcgta tctctcttcc agctattttg agcccctgga
    19261 tctgactttg ctcataggct ccttcggagc atcagcggta ctcgtatatg ccgctatcaa
    19321 gagccccctt gcccagccaa ggaaccttgt gggtggtcat gtgatttccg gacttaccgg
    19381 agttgcctgc ttcaacctgt tcggaggcac cctttggata gcggcagcca tgagcgtgtc
    19441 ccttgccatt gcgatgatgc ttatcacaaa gacgctccat cctcccggtg gcgcaacggc
    19501 attaattgca gttatcggcg gtgaaaaaat acataatctt gggttcttgt atgcctttgt
    19561 ccccgcaggt ctcggcgcca tcgttctttt gattgtcgcc cttctggtga ataacatctc
    19621 aaagaacaga agatatcccg agtactggtt ctgagtgaac ctcccgcttt acatgtcaag
    19681 acagaggtca cctgtctgag aaatgcaccg accaatcgtc acaggaaaca ggtcgggctg
    19741 aggaatttac ctgaaagaag tccgcgacag gtttaattta ccacaactga ggagtacccc
    19801 cgcgcacata gtcccatctc acttgcaaag gaaaaaatcc ccaggatatg gctataatga
    19861 atggtcccgg gtgcaaggag agttatctga atgaagagaa gaattcttga aagttctgtc
    19921 ttgtttatca gtatcagtaa atggatcttc ctcgcttcct gcgtaggggt gatggttggc
    19981 ttttcgacca cagtattcct caaactgctc ggccttggaa ttgcatccgc atcaggattc
    20041 aaatactatt ttctccttct tcccttcgca ttttttgcga gtgtcatcct cgtgaagtat
    20101 cttgcgcctg aggcagaggg gcatgggaca gagaaggtga tagaggcggt gcacaagagg
    20161 gcggggaaga taaatcctat ggttgtcccg gtgaaactcg ctgcaacaat cattacgatc
    20221 gccctgggag gctctgcagg caaggagggc ccgtgtgcgc agataggtgc cggcctttca
    20281 tcgggtctcg cagaccttct gaagttcaat gacaaggacc gcagaaaact ggtcatctgc
    20341 gggatcagcg ccggttttgc cacggtattc gggaccccta ttgccggtgc tatcttcggt
    20401 gtggaagtgc tctttgtggg aggattgctc tacgatgtgc tccttccttc tttcgtcgca
    20461 ggcattgtcg gtttccaagt ctcatcgtcg ttgggcatca cctattttca tgagcccctt
    20521 aattttgtcc cggtcttcag cagttttttc ttcataaagg tctgcgtcgg aggaatcttc
    20581 ctcggactct gctcctttct ctttgtggag atgctcaagt attttaagga attgagcggt
    20641 aagcttaaga tgccgatgca atataagggg ctgctgggtg gatcaaccct tgcactcctg
    20701 acccttctcc tttcgacacg gtacctgggg ctcggcctca acagtatcca gacttccctc
    20761 acggggggcg aaataccaaa gcccgctttt tttctcaaaa tgctcttcac ctcgatcacc
    20821 ctcaatttcg gcggcagcgg cggcatcgtg acgccgatct tttttgtggg cgctacagcc
    20881 ggcaacatgt tcggcagcgt ctttggatta gaccctgcgg tcttcgccgc cattggcatg
    20941 gtgagtcttc ttgcgggcgc tgctaacaca cctatttcgg caagcatcat ggccatagag
    21001 ctctttggtg cgtccattgg cccttacgca gcagtttcct gcgtgatcag ctttctgatg
    21061 accgggcata gaagtgtgta tcccagccag gtgctatcga tgaggaagtc ctcatcgata
    21121 gcagtagaaa aaggcagggt catggaggat atcgggaaga tagatgttca gataagaaca
    21181 aaaagtgttg taggtcagat agcaaaggcg ctgaagaaaa tgagaaaaat aacatagaga
    21241 atgcctcttt ttatgtaatt ttctttcgca cgtctctggc agtttatttc ctgcgtgaaa
    21301 agggtaaaat aaggtaatac gtatgaagct gccccctcat aaaacaaaga ttgtctgcac
    21361 tatcggaccc gcctccgagt cgccggctat gatggaagag atgatcattg cagggatggg
    21421 cgtggcgcgg ctcaactttt cccatggcgt tctggaagac cacggaaggg tcatccagaa
    21481 cctcaggctt gccgcgcgca gaaccggaca acgacttgcc atcatggctg acctcccggg
    21541 gcctaagatg cgcatcggcc agctggccag ggagcccata acccttaagc ccgggggctc
    21601 cttcaccctt accagtgaag agattgtggg tgactccaac cgtgtctctg tgaccttctc
    21661 ccgtctcccc caggtggtga aggcaggcaa cacgcttttc cttaatgacg gcctcatcca
    21721 gcttgaggtc gtaaaggttg aaggaaacaa cgtcatctgt cgcgttgtgg tgggaggtga
    21781 gttgcgctca agaaagggtc tgaaccttcc tggcatcgat ctcggcatca gcgccttcac
    21841 ggaccgggac ttccagtgta ttaaatttgc ccttgagcat ggtgtggaca tcatcagcca
    21901 gtcctttgtg cagtccggtg ctgatgtgtc ggctgtaagg cgttcggcaa cgtcccttgg
    21961 gtacagccct tttattatct caaagatcga gcgctccatc gcctttgagc atattgaagg
    22021 gatcctcaat gagaccgacg ggatcatgat tgcccggggc gatctcgggg tggaaatacc
    22081 catagagaag attgcccttg cccagaaatg gctcatgcgc aaggcgaaca tggcagggaa
    22141 gcctgtcatt accgccaccc agatgctcga atccatgatc gggaaccctc gtcccacaag
    22201 ggcagaggcg actgacgtgg cgaatgcgat cctggacggg acggattgtg taatgctctc
    22261 agaggaatcg gccatgggca ggtatcctct tgaagccacc tccatgcttg ccaggatagc
    22321 tgccaccatt gagccctacc accctgccca ccatgtgagg gaggcactca ggagggcctc
    22381 gggtcaggaa gaaaccatcg gggtgaagga cctcattgcg catagtgtcg agacagcggt
    22441 ggagcggatc tctcctgcag ccatttttgt tcctacccac accggagcaa cagcgagaaa
    22501 cgtcacccgt tacaggttgc ctgtctggat cattgcggtg agctcccagg agaacacatg
    22561 ccagtgtctg cagctttcct acggcgtata cccccaattc gaggcagtcc acccggagaa
    22621 ctggaacagt tatgtaaagg actggatgtc cgcgcatgag cttgagggga atatgatcgt
    22681 tctgatagaa ggtccttctt cgcgccatcc cgaggcaaac aaccgtatgg agattatcca
    22741 gctcgaccgt gtagtatagg ctgccaggtt tttggcgttc gattttcgta ctgtaagacc
    22801 gccaaagttt gtcgattctt gaccgtaaaa taaataacaa attgatttta aataagtttt
    22861 ttattggtac agaaaatgct tatcaactcc ataaggaaaa agggtaatat ggaaaattga
    22921 ccttgagaca agccatgctg aacaggagac aatgcaagag atttccggag cactgtaaca
    22981 ctgaattcgc gtctgacggg atcacctatc atgcaatctc gagggacttc gcgttgagcg
    23041 gacttttcat agtgacaggc tcgcccaggg cacccggcac actccttcag ataaagatcc
    23101 gtctgcctga cggctcaact gccctgttac tgggaaaggt catgagattc tacaagacgt
    23161 ttggaggaaa cgacgtggcg ccggttcagc aggcggaaga tggaatgggg atcgagattt
    23221 tagaaaaaga tttgaattat cttcgtttca tcaggtctct gctcgagacg cccgggaaac
    23281 acgaacccga caattgaacg tgctggagga gaaccatgcg agacaaaaga caatctacac
    23341 ggttcgaaaa gagatgtgaa acggacttcc tggccgaggg aatcactcac aggggaatat
    23401 gccttgatct ctccctgaac ggtctgttca tagagacgga ttttcgattt tcccctgata
    23461 ccatgctgaa cattcttatt catctccctg atggtacgat ctcaaaacta aaggggaaag
    23521 tggtgaggtg cctggacaat ggcattgggg tggggattat agaaaaagat gcctcttacc
    23581 tgcattacta cagccgttgt ttgttggagc ccgaggccca tgcgccttta agggagccct
    23641 agacccgata actcataaat tcggcagggg tgtccggctc gaaggacaat ccgcctgagg
    23701 cggagggtta tatgccggtc tgcgagagcg gaccttaaca atactcaggg acagagtgtt
    23761 gataagtgca gagactcccg ttaaggtgag aagggctatt gtcttgcctg aagacggatg
    23821 cctctgccga agcaagaaac tcatgaatga agtcgcccag acctgcctcc cttctcgcga
    23881 ccttctgaat ggtacccacc ttcagtcatc ccgctctgat agaataaaag atatccatga
    23941 aggcgatgat cattgaatca ctcaatatcg gcctgccgaa gaaggagcgg tttcatggca
    24001 gggaaatgct cacagggatt tgcaagatcc cagtatccgg accattgctg ctcaataagc
    24061 tggggtttga cggcgacggc gtgggggata tgaaacacca tggggggcct gacaaggctg
    24121 tatgtgtata tagcacagat cattatccat actggcagga gattatggga atgaaactgc
    24181 cggtcgccgc atttggcgag aacctctcgg ttgcgaatct gcgtgaagac gacatctgca
    24241 taggggatat ctttgaactc ggaacagccc tggtacaggt gagtcagccc cgtcagccgt
    24301 gtagaaccct tgccgcacga tacgcgaggg atgacatggt cagactcgtg gtggactcgg
    24361 gccgtacagg attttactta agggtgctga aagagggcca ggtggaaaaa gacagcacgc
    24421 ttattttgaa agagagagac ccccgcagga taaccatagc attcgccaac cagacctatc
    24481 atcatgacaa caagaattgt gaggctatca aaagaatcct taaagttccc tacctgtccg
    24541 aatcctggaa acgctctttt cacgaacttg caaagagatg ccggtagccg cgctctcaag
    24601 caagcatgga tcaaaaatcg ggaaacatct tgagcgcaac acccttgccg tctcccatag
    24661 atgcgtgtgc tgaaaggatg cccccttcta tatactggag ggtaaagtcc ttgaagcccc
    24721 gtgccacaaa gcccttggca tttgcaggac atccgcgttc gcatcccgct attccttcga
    24781 tcattcttgg gatcagcccc cgtatatcaa agccgctgat gtcaatcgcg tgcgacgagg
    24841 cagcccggtc aatctgaccc atgatcgcgt cgaggtcagg gtgaaagggg tcttcaatct
    24901 gtattgagac cgatctgttg ttgacaacca catcaagcac catagtatct cctcccttgc
    24961 atggtctctc ttaacttccg gcatccactg tctttatcga gagctctctc agctgtttct
    25021 gctccacacc ggacggcgcg ccgctcatga ggcatactgc tttctgggtc ttgggaaacg
    25081 caatgacatc acgtatggac gatgcaccca ccatgagcat cacgagcctg tcaagaccga
    25141 gagcaatgcc tccgtgggga ggagccccga actccagggc gtccaggagg aaaccgaact
    25201 ttacccgcgc atcctcgtct cctattccca ggacctcgaa catctttttc tggacatccc
    25261 ttctgtggat cctgatgctc ccgcccccta tctcataccc gttcagcaca atgtcatagg
    25321 cttttgcctt aaacgcagcg agttgtgaac gttgggcagt gtacatttca ggagaggaga
    25381 gatcgagtga acgcaatgtc tcgagatcat cttcagccgg cgacgtgaac gggtggtgca
    25441 tggcctggaa cctcccctca tcctcatccc attccaggag cgggaaatcc agaatccatg
    25501 cgaacttgaa caccggctgc atgaggttca gattcttccc gaactccagc ctcatgcggc
    25561 tcaggacatc atggaccacc ttgtctttgt cagccacaaa gagcatgagg tccccttcct
    25621 cagaatcaag cctctgggcc atctgcctca atatctcctc agggaagaac ttcgcgatgg
    25681 gggattcaaa gccgtctttc acctttatcc atgccaggcc ctttgcgcca aaagccttcg
    25741 cctcttcggt gagggtgtca atctccttgc gcgaaaaccc ggccatacct ttgccgttta
    25801 tggcctttac cctcccccct gatgccatgg catcaaggaa gaccttgaat gagccgttct
    25861 ttgcaaggtc agccatctcc ttcaattcga gactgaaccg caggtcaggc ttgtcattgc
    25921 cgaacctctc catggactcc ctgaaactga gcctctcaaa aggcaccgaa atatctactt
    25981 taaggacatc catgaaaagc ctcttcatca tcccttcaat gagatgtata atatcttccc
    26041 tctccacaaa ggacatctca aggtcaacct gggtgaactc aggctgtctg tctgccctca
    26101 ggtcttcgtc cctgaaacat ttcactatct ggaaatactt ctccagtcct gagaccatga
    26161 gtatctgctt gaagagctgg ggggactggg gaagggcata gaactgcccg gggttgagcc
    26221 tgctgggaac caggtagtcc ctcgcccctt caggggtcga cttggtgagc atcggggttt
    26281 cgacctcgac gaatccccgt tcgtcaagat aatcacgcat cacctttgtt gccttgtgcc
    26341 tgataataag attctgctgc agctcgggcc tccgcagatc aaggtaccgg tatttcagtc
    26401 tcaggaattc cgaggcctct gcggcctctt ccatgggaaa gggaacaggc gacgactcgt
    26461 tcaggacagt gaggctgttt gcgtagagct ccaccatgcc tgtgggcagg ttcttgttca
    26521 ctgttccttc aggcctcctc ctcacctcgc ccgatacatg gatcacaaat tcagccctga
    26581 ggtccccgcc ctgtgtatgt gcatccgcgg acacatccgg gctgaagacc acctggacca
    26641 aaccgcttct gtccctcagg tcaacaaaga tcaatcctcc gtggtccctt ctcctgaaga
    26701 cccatcctgc gaggctcatc acagagccta tgtcagactc tctcagctcc ccgcatccct
    26761 tatccctcat cataacgcct cctgtctgct catgagtgaa gtcttgtgtc tatatcccga
    26821 tctctttttt tataagcctc agctcatcag gggtgagaag cctcatttcc cctggcttca
    26881 ggtccccaag tttcaggccg tttacccctg tcctctttaa tttcagcacc aggtgcccca
    26941 ccttttcgat catccttctc acctgtctgt tccttccctc gtgtatggtg atctcgatcc
    27001 atgaattgtt ctccacctgg cgtatcttct ttacccttgc aggcatggtg acgccatcat
    27061 cgagcttcac tccctgcctc aatttctgta tgttctcttc ttccatgacg cccttcacct
    27121 tcacaaggta ggtctttggt atcttttgcg acggatggag tatcgcattg ctgaaatccc
    27181 cgtcattcgt caacaagagc aggccttcag aggcgtaatc gagccgtccc acagggaaga
    27241 cccggtattt tacacctttg agaaaatcct ttacagtcgg ccttccctca gggtccgaga
    27301 gggtcgtcat cactccccgg ggcttgtgga acatgagata ggttttctgc atctctttcc
    27361 ctgaaccggt gacacgcttt ccatccacct ttatatagtc cctggacggg tccgccttca
    27421 tcccgatggt cgcaatctgc ccattgaccg tcaccctccc ctcaaggatg agctcctctg
    27481 cctttctcct ggacgcgatt cccagctcag agagtatctt ctggatacgt ttttccatgg
    27541 aactaaatga taccataaaa aggagcgtac aggcacaaaa gggcgctggt attgaatccg
    27601 cattcactgc ataacaaggc atgctttggc cctttttatg gtaatatggg aaactcatga
    27661 agagggaaaa ggagacaaag atcctgaaaa ccgcaaagcc caagaccgcg agttggaaag
    27721 acaacatctc cttcatcctt gtagagccaa gagagccggg gaatatcggc gcctctgcaa
    27781 gggcgatgaa gaacatggga tttacgcacc ttgaactggt gaagccccgc agttttatga
    27841 ctgaggagac aaagaaaatg gcgtgcaatg cctttgatat ccttgccagg gctgagattt
    27901 acgggagtct taatgaagca ctggaggcaa agaccttggt agtgggaacc acaaggaggc
    27961 tcggcaggac aaggggtctt atcctccatc tggaagaggg aattaaaaag gtgatcgcct
    28021 ctgccaaaaa aaacagggtc gccatactgt ttggaagaga ggacaagggg ctgaataacc
    28081 gggaagtgga ggaatgcggc ttcctcatca ccatcccctc agacccctcg tccccttccc
    28141 tcaatctcgc gcagagcgtg ctccttgtgg cgcatgagct gagcaggaaa acttacagcg
    28201 gccagttccc cagagcagta acgcggccag aactggaaat actccaccac cacatccatg
    28261 acaccctcaa gctccttgag tatattccga gggggaacag ggaccttgag gcaaggatca
    28321 tgaggaatct caaacacctc ttggcgaggg cagggcttac tgactgggaa ctgaatatgc
    28381 tccacgggat ttgcgggcag atagagaaaa agatggggca gtaagagagg atagggtaat
    28441 atttaaatga aacgggcccc aactttatct ccaagtgaca aatgataaaa acactcgtat
    28501 gcattgatag agatggaacg cttatctacg atgaaaaaga gcacctctat ctcggaagag
    28561 acaatgattg gaaagctaag gtcaaaatac ttccctatgt aattgagggc atgagggcac
    28621 ttaaagccat tcccgatgct gcagtgtaca tgatcacaaa ccagcccggc gttgcgataa
    28681 ctgatttccc gttgttgact ctggaacggg ctcatgaagt gtgcaggtat attcttgaga
    28741 agataaagga tataggaggt cgtattgacg gatatttcct gtgccctcac gcaaatcagg
    28801 cgtatgtgaa gaaatataca caatatcatt tcgataaaag acttgtgtgc gaatgcagtt
    28861 gcataaagcc ggatcttggg atgatatttg acgctttgcg gtcggaaggg gtcaccccgg
    28921 gcagtgtaag catctatatg atcggagacc gggcaaccga cgtacagaca gcattgaata
    28981 tgggcgggac aggcattctc gtgccttttg aaaaccagcc gggcgaggat caggaggtca
    29041 ataaaatcat ggatcagtcc tgcatctata ttgccaaaaa tctactggat gccgctgaat
    29101 tcatagctct tcataccagg gcaggaggag ggtaaggcgc tgactcgcag gacatgaatc
    29161 gctatcctta actttataac cttcaaaaac ctgagccgtc ccgacaagtg tgaaactgag
    29221 tgaccggcgt tgcataagcc accaaacctg caataataaa aaaaaacgac tctttaatta
    29281 agaggaaagg ggtaacggat gaagcataac agatatttgt cttttgtaat aggcatttcg
    29341 ttttttgcaa tgctgtttct gcctggaatt atgaacaatg ttcaagcgca gggtaaacag
    29401 gaaacttcag tcgaaggaag attaggggag acacccacaa aagaaaagtt ctctgaagaa
    29461 aagagagaat tcaagagtaa aacccaaaaa agacttaaag aagtggacaa gaaaatcagg
    29521 gaacttgaag cagaatcaaa gaaagccggc tcaaaggcaa aggaagaggc caaagagggc
    29581 ctgcaggacc tgaagaagaa acacgctgag ctaaaaaaag aaatgagaac acttgaggca
    29641 aaaagcaaaa aagagtggga aacggtgaag cagaaagttg gcgctgcaat ggacgaactc
    29701 gaggaggcat acaacaaggt gcgcaataaa ttcaagtcgg aataaataaa ccaagaacgg
    29761 ttaatgtgat aaatagcgaa gaggggggcg aggatcttat cactttgtat tggtaacagt
    29821 gagcggggat aaggtgaaag gagaggtaat ggacgtcacc aggaaagtaa cgtgtacctt
    29881 ccggttgcaa gatgtttgtt tagctcgctg aagctttcct gaatataggt gcgttcagag
    29941 caggcgcgca agcaggtgag ccaggaactc tctcatcctt gaatctaaag gt
//
LOCUS       DATKOU010000219        40912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4154797, whole genome shotgun sequence.
ACCESSION   DATKOU010000219 DATKOU010000000
VERSION     DATKOU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 40912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40912
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4154797"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VMU21_10345"
     CDS             complement(<1..375)
                     /locus_tag="VMU21_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77962.1"
                     /translation="MKRKRAIMNRKGDPALTPFMQSILRPFTNIANAITKDRYKSLKL
                     RLFFYIGTLAALPLIGIAMMSYFWVFNILKDEFRAHLRWQIEDSQRSIESFLQEKLSG
                     LRFLANAYSLNELSDDAILHTVF"
     gene            468..3035
                     /locus_tag="VMU21_10350"
     CDS             468..3035
                     /locus_tag="VMU21_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HUO77963.1"
                     /translation="MKLWQHISGLLKAEETHGKKKDEGSVVLTQKFCAFQRLLSSNNA
                     ALELMADMGEKLSGDFLFDRHYVDSAVSAVSERVKDLVENLRIISKGKYQALDERFDE
                     IQSGIQKILTQKKEIAESPYTLSFGEITREKAEMVGSKVANICEIRNRLALPVPDGFG
                     ITAFAFKRFMEQNDFFGKIKEKLDDLSIDDLEALNATSREIQDLIAGGEIPSDLEAAI
                     AASYLSLCEREGSRVKVSVRSSALQEDGEFSFAGQYSTFLNVPFESLFEKYKCVMASL
                     FNPRALFYVKTKGFQESDMVMSVGVLTMVDAGAAGVVYSADPNDPERDVIIISAVKGL
                     GVGLVDGTITPEIYLVSRSGGEIIEKSLSKQSHMLACNSTGGLEEIVLDERHDGKAAV
                     ADEEIKRLARYALSLETHFQGCQDIEWAIDRHKGLCILQSRPLRVLARKASVAVPTQV
                     KGYTVLIDKGAIAFKGIGFGKAYIVRQDEDLEDFPEGAVLVAKHTSPKFVAVMNRASA
                     IVTDVGSATGHMASLAREFQIAALLDTEVATSLIQQGEEITVDAINCNIYKGHVAELE
                     EFAAKRENLFKETRIFKTLEDVLKLISPLYLVNPDDKAFKAESCRTYHDITRFCHEMA
                     IYELFDVTGISADEVGAIRLVAGIPADIYLIDLGGGTSGKILEDTKNLLLKDISSIPF
                     NAFLRGLTAMKWPEPRPADAKGFLGMLATTASIPEEELKRTAEKSFSIISGDYMNFAI
                     RLGYHLSTVEAYAGENINDNYIKFTFKGGGAIIDRRLRRIRLITEILRRLDFSVKVVE
                     DVVNASLMKYRRSSTEKTLEILGRLTVYTKQLDMVMYNDAITDVYIEEFCSAYVTGH"
     gene            complement(3029..3454)
                     /locus_tag="VMU21_10355"
     CDS             complement(3029..3454)
                     /locus_tag="VMU21_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77964.1"
                     /translation="MPEKLLIVDDEPDMLRLLQMIVREKTAYEVTTTNNPLEALGLAR
                     KGGFDLLITDLKMPGLDGIELLKTVREFDADIPIIIITAFATSEAADAAMEKNAFDFI
                     TKPFRKEQILFTIEKAIKWLRTQRENKMLRESLGKEPSQ"
     gene            complement(3470..4567)
                     /locus_tag="VMU21_10360"
     CDS             complement(3470..4567)
                     /locus_tag="VMU21_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO77965.1"
                     /translation="MEADTPMNILLVDDEVDFLNVLSKRLEARGFVIDTATNGNTAIA
                     SAKDKAFDVIVLDLSMPDIDGIETLKRIKKDNPDMQVIVLTGHATVEKSVDAMKEGAV
                     DFLEKPVDLSKLIEKMKVAHEIAQKKKIEQQMAHTEKLASLGTLAAGIAHEINNPLTI
                     ILGFTDLMLEKVPQDSEFYELLKAVERQGQNAKRIVENLLGFARYTEYREEDVDINKN
                     IRSVLAVVESVLTTNRISVNLDLSGSLPIIRGSFGELQQVFFNIINNAIHEMKGGGTL
                     TVASRAVDEGKRVEIQIADTGPGIKQRERAKIFDPFFTTKKVGEGTGLGLSIAYGIID
                     KHGGTISFETRTRDESNAPGTKFIITLPVVK"
     gene            complement(4695..5135)
                     /locus_tag="VMU21_10365"
     CDS             complement(4695..5135)
                     /locus_tag="VMU21_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77966.1"
                     /translation="MKILVPVDGSRYSREALKVAVEFAKTRGAEICLLNVVTDFTDID
                     LEISAGERDRVTQRLESRGESIIQEARRMLEAEKVSADCRSNVVATSITDAIIDVAEK
                     EKMDLIVIGSRGLSKSSRFKLGSVASKVVAYAPCSVYVVKLTSE"
     gene            complement(5146..5895)
                     /locus_tag="VMU21_10370"
     CDS             complement(5146..5895)
                     /locus_tag="VMU21_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77967.1"
                     /translation="MPVRHGKHLSLGIVLAISFLVVLFLIFSPLFSIGEKKVNGLEWS
                     DEMFNRLAKGSSYFIPKVVKSSEKFKGRMFSATIRMDKPEDKPGEAEKRAERSAKVLG
                     VAGAKTELKGSELRIEGDLGQVLEAALQDADTMFKNEGDKIKAKYAYDDEKQMFRQWN
                     NTLPKISKQFEKEKKMEEAKIITDVVKKAIEPSYNFYNVKAVEVKDHIVLMTFLLVFY
                     VAYTMWWGFSIFYVFEGLGLSMKKAKAKKEV"
     gene            complement(5898..7166)
                     /locus_tag="VMU21_10375"
     CDS             complement(5898..7166)
                     /locus_tag="VMU21_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO77968.1"
                     /translation="MNAPRRMFEFLKMASIAHAEWDYEVSMSIVKSRKKLLILFILLL
                     PIVAISLLDAADVIGGKEAYSPAHYSTAIFIVSIAIGLGAGLITGCIGAGGGFIITPA
                     LMAAGVKGIMAVGTDTFHIFAKAIMGTVVHKKLGNVSLGLAVAFVCGSAIGATVGGLI
                     NRTLYNIDPAMSDVFISVVYAVILGFLGTYALVDYLKLRKHSEDAGAHGGGAHGGGAP
                     KTTTGVAIKLQALKIPPMIKFDEDIVPGGKRISGVIMAIGGSIVGFLAAIMGVGGGFV
                     TFPMFVYTFGISTMTTVGTDILQIIFTAGYASITQYAIYGYVFYTLAMGMLLGSLVGI
                     QVGALTTKVVKGIHIRAFYALTILAGFANRITVLPKKLGDLGYVKISKNMADGIEAVG
                     FWVFWIMIGIFGVWLFAKFFGNIKVLREEG"
     gene            complement(7201..7941)
                     /locus_tag="VMU21_10380"
     CDS             complement(7201..7941)
                     /locus_tag="VMU21_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HUO77969.1"
                     /translation="MQIEKTVDRDSEQKLYVQIYTIFKAKIESGEWYCGVRLPSEDEL
                     CRAYNVSKATIRIAIAELVRDGYLRRQQGKGTFVTRSIAHSGMTMRTRLTEDMFGEGV
                     KTRKELMEKGIKEPTEKIKRYLKVEGDFYRVLCKRVVEDEPAYLEESFIPEALFPGIE
                     REDLCSTSFYDLIQEKTLRRISKVLQTIEVTEITGDAAHILEVKEGSPGLLLHRLLIG
                     SDGIPIAYTSLIGSGVKYKIQTEFERIK"
     gene            complement(8013..8297)
                     /locus_tag="VMU21_10385"
     CDS             complement(8013..8297)
                     /locus_tag="VMU21_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77970.1"
                     /translation="MKRLMKMFEHMMVSITFAESGEYDLVKEMIADEWRSVEVSREGS
                     EEAVQPVRGTVQMDKDLRMELKYVLLACLVTLISTYLFCQFLPITPFECW"
     gene            complement(8507..9787)
                     /locus_tag="VMU21_10390"
     CDS             complement(8507..9787)
                     /locus_tag="VMU21_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO77971.1"
                     /translation="MLNILVISDDAKKIYAMLKSHGFVPQKEHQKQKDVSFAYDIALL
                     DLDSQGWQQKLIELRQRMPVIALSAPDVRLAVEAMKLGASDFLGKPLTLASLGDVIKG
                     HGKKILDHKHGFDEIIGISPPMQEVFGLIRKAAVSESNVLITGASGTGKELVARAIHR
                     WSPRSGRAFMTINCSAIPDTLLESELFGFEKGAFTGANYTKKGLLELADGGTVLFDEI
                     GDVSPLFQVKILRVIQEGEIMRIGGSRPQKIDVRIIAATSRNLKNDCQKGIFREDLFY
                     RLNVINIHLPPLRDRIEDVPLLAEHFVKKHATKRKDFLIRGISDDALSILLDHSYPGN
                     ARELENIIERAISFANSPVIMPSDLPSCLAQAAPRKAASSRNLKNALENLERELIWSA
                     LQKSRGNISKAADELGIYRQQLQRKIKQFKIATL"
     gene            complement(9882..10046)
                     /locus_tag="VMU21_10395"
     CDS             complement(9882..10046)
                     /locus_tag="VMU21_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77972.1"
                     /translation="MKEKEGSDVLSASLSRNIERISIPASLSFSLYLGGGTIPKLLIK
                     GKAMFPFYLN"
     gene            10491..11399
                     /locus_tag="VMU21_10400"
     CDS             10491..11399
                     /locus_tag="VMU21_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HUO77973.1"
                     /translation="MSGLQWFMVLFGWFSLVVFVVGAAYKAVKFSKMPLNLRWEVYPV
                     PHEEGEKRHYGGSYMEEMDWAKKPLPSSLAGEIVEMGSEIFFLKKVRVYNPYNLWFFS
                     MAMHWGIYLLFGWVFLLVIGAILKVDALSQITGIVGIVSFVLGAFGCLTLIIKRATNS
                     ELKLYTEPVEYFNLLLLFAIFVTGIMSWRVDPSFAGSRDYVGGAIFFNPTPVPSIVLL
                     NFLLFEIFLIYMPFSKYLHYLAKYFNFHKSLWDDAFKQKGSPKDLKNIEQVGYTVHWA
                     GPHASPDETWLENAQKWLLEKKEEKK"
     gene            11396..13000
                     /locus_tag="VMU21_10405"
     CDS             11396..13000
                     /locus_tag="VMU21_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUO77974.1"
                     /translation="MKNQVSFRDIARLTDEPLINLEVKDLMELPVEEPPMEPPKEKWL
                     ESYDFSLDGHTPFDYPKPKTQEEKEKLVFGFMSGLEKLFNREANWTFLKIFKLSMDYC
                     VKCQTCSEACHTYLASGRQEIYRPTYRSEILRKLYKRYFTPSGKLLKSFVGADVEANY
                     KTVWRLLELAYRCNVCRRCASYCPVGVDNALIAREIRKVFSQELGIAPLELLRKGSRQ
                     HLAKGSTTGMTPAACKATIEFLEEDMEERTGRKIKIPIDKKGADILLMQNAGEFLAWP
                     DNPEAFAILFDAAGINYTLSSEMCGYDAVNYGVWFDDIEAARVGLKHLSIAKDLGVKK
                     IVVGECGHAHKAMCVLLDRIAPGDFALPLLPRESCLPLFWDIVKSGRVKLDPSRNNFP
                     TVLHDSCNVVRLMGIVKPQRNTLSAICAQPYREMNPHGPMNYCCGGGSGFAIMNSLNF
                     PQFRKKVTERMKVKQILEAFNDVLDPSINKMVVAPCSNCKGALRDALHYYGLWEHHRI
                     LYSGLVELIVNAMVDIPPFLKWEWEE"
     gene            complement(13193..13390)
                     /locus_tag="VMU21_10410"
     CDS             complement(13193..13390)
                     /locus_tag="VMU21_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77975.1"
                     /translation="MPRLIAAMVFLAQLLKLCMAVMTGRYDHIGMCACNLIELESAIG
                     EFLVPTSGLMNSSSNYDKKIE"
     gene            complement(13494..13844)
                     /locus_tag="VMU21_10415"
     CDS             complement(13494..13844)
                     /locus_tag="VMU21_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HUO77976.1"
                     /translation="MEPLATRYIEVQGKTIEVDEDGNLKCAEDWCEELAIYLSKEDGI
                     ELSKDHWDVINFVRWYYITYQNCPHPKFIVKNLNKRLGDGKYTVKSLYDLFKEQPVMK
                     ACKYAGIPFTAGCT"
     gene            14161..14490
                     /locus_tag="VMU21_10420"
     CDS             14161..14490
                     /locus_tag="VMU21_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77977.1"
                     /translation="MKSKNMYVIFLFGVIIIVRVLMKKNCWEYSRCGRERGGRNEKEL
                     GICPAAAEERLNGVHGGINAGRACWAVAQVFCDCTRCNFFQLVKEEEGTNYETSLALL
                     KKLTTSK"
     gene            14774..15142
                     /locus_tag="VMU21_10425"
     CDS             14774..15142
                     /locus_tag="VMU21_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77978.1"
                     /translation="MADTPRGTYFFIFYRDLRAHEGRMVKELGAQRANRYREGNAFFE
                     VHLLKVGPPIILGFASESDALRVSSPSTQIQKISIASAPWEKMTSLLSLPADRIVSAE
                     TRTVSPSEREWMYILDCDIR"
     gene            complement(15380..15438)
                     /locus_tag="VMU21_10430"
     tRNA            complement(15380..15438)
                     /locus_tag="VMU21_10430"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(15939..16223)
                     /locus_tag="VMU21_10435"
     CDS             complement(15939..16223)
                     /locus_tag="VMU21_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77979.1"
                     /translation="MGVISLRLKDRDMERLEELSMSERKDKSAIARELLEYGWDFLML
                     KSYKEGKLSLTGLAKKLDISISEAIDLLKELGIEAPIGFDDYLKGFEVFD"
     gene            complement(16223..16711)
                     /locus_tag="VMU21_10440"
     CDS             complement(16223..16711)
                     /locus_tag="VMU21_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO77980.1"
                     /translation="MVFDSSTLILLAKIDLLREVTEDIHIIIPQKVKEECLLKESPDA
                     SAIKALIMEKKIKVEKVGNQEVIRKLQIDFRIETAEAQALWLSRSIGCPLAVDDGPAI
                     KACKVLGLQFATAVHFLLNLRTRKKLELPMALAKLERLSGYGRYNKRIIEDATERLKG
                     VK"
     gene            complement(16832..18022)
                     /locus_tag="VMU21_10445"
     CDS             complement(16832..18022)
                     /locus_tag="VMU21_10445"
                     /inference="COORDINATES: protein motif:HMM:NF023223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HUO77981.1"
                     /translation="MNWTTPADLRAQVERLWNKGFLLASMVDGETLFPRRLTLKKPTS
                     SELADRFEDVRTWIAELLSVRHYRIVMREVRHHVIGSNAVPDEIWIDTPDDALALIGK
                     GRDARRFGELVELTRERQPAILPWLSRRPLKVLELAEDWRHLLNIIDWMRAHPRPGLY
                     LRQIDIQGVHTKFIEAHRAVLSGLLDLVLRPETVDTETGGVNQFCRRYGFLDKPLRIR
                     FRVLDPALALLPGSADQDITVTQDAFSQLDSRVGRVFITENEVNFLAFPNLSDSMVIF
                     GAGYGFEMLAQAKWLHQCAIYYWGDIDTHGFAILDQLRAYFPHAQSFLMDRETLMAHD
                     LLWVIEPQPLSRDLVRLSQEERALYDDLRDNRIGSSIRLEQERIRFGWVVTALVSLDQ
                     QSRI"
     gene            complement(18019..21387)
                     /locus_tag="VMU21_10450"
     CDS             complement(18019..21387)
                     /locus_tag="VMU21_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HUO77982.1"
                     /translation="MNESPDLEFMADDSLSGFRLHRLEVFNWGTFDGRIWTLHLNGRN
                     ALLTGDIGSGKTTLVDAVTTLLVPAQRIAYNKAAGSETRERTLRSYVLGHYKSERSEL
                     SGTARPVALRNHNSYSVVLGVFYNTGFNQTLTLAQVFWMKDPQGQPARFFVGATRALS
                     IETDFSQFGSEIGALRKRLRGAGAEVFDSFPPYGAWFRRRFGIDNEQALELFHQTVSM
                     KSVGNLTDFVRSHMLEPFDVDPRIKALITHFDDLNRAHEAVLKAKQQVALLTPLLADC
                     DRHAEVTVRSEELRACREALKSYFAKLKLGLLDKRIASLAEEWSRQNASVQRMEERKN
                     RQLAEERELRRSIAENGGDRIERLAAEIRQKEDECVKRRSKAARYAELTGSLNIAAAD
                     SAEAFLDQRKTFGELADKASGREAALQNELNEAGVTFAQWRQEHDALSAEINSLKSRR
                     SNIPVEQIAMRTAIASALKLTESDMPFAGELIQVREEEQKWEGAAERLLRNFGLSLLV
                     PDKHYAQVAAWVDRTHLKGRLVYFRVRKAGRSDVSALHQHSLVHKLAIKPDSQYYDWL
                     EREIAHRFDVACCETQDQFRRETRAVTLAGQIKVQGERHEKDDRHRLDDRSRYVLGWT
                     NKAKIAALETKKQQLETRLTGMGSHISSLQKEQRELRSRIETLNKLNEYQDFQELDWQ
                     PLAAEVSGLQQQKRQLETASDLLKELTGRLDALTLEIQETERLLIEGRDKRSRTEQKK
                     TDAETLYAQAQALLDDPLNAHNSARFESLEAMRPEALGEHYLSVESCDNREQEMRTWL
                     QSRIDNEAKNLTRLRDKIIDAMRSYCASFPLETQEVDVSVEAVGEYRLMLEKLNADDL
                     PRFEARFKELLNENTIREVANFQSQLNRERETIKERITHINESLTQIDYNPGRYIVLE
                     AQTTQDADIRDFQGELRACTEGSLTGSEDEQYSEAKFLQVKRIIERFRGREGQSEADR
                     RWTAKVTDVRNWFSFAASERWREDGKEHEHYSDSGGKSGGQKEKLAYTILAASLAYQF
                     GLEWGAVRSRSFRFVVIDEAFARGSDESAQYGLKLFQQMNLQLLIVTPLQKIHIIEPF
                     VASVGFVHSEDGRSSKLRNLSIEEYQAEKAKAQA"
     gene            complement(21380..22021)
                     /locus_tag="VMU21_10455"
     CDS             complement(21380..22021)
                     /locus_tag="VMU21_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HUO77983.1"
                     /translation="MAEQDFGMQPSKDYDLSSVVVPLLKGVLYQESSPDLWNSLLGLQ
                     ARVRDYVSVIGLDLVLDEAEGYAFLRSHSGNGDETETKLPRLVARRSLSFPVSLLLSL
                     LRKKLAEFDASGGDTRLVLSGEDIAELIRVFLPESSNEAKLMDQIDGHINKIVDLGFM
                     RRLKPQSGQKEMFEVRRILKAFVDAQWLAEFDERLADYRTLLQEGRKGSRQDE"
     gene            complement(22024..23469)
                     /locus_tag="VMU21_10460"
     CDS             complement(22024..23469)
                     /locus_tag="VMU21_10460"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HUO77984.1"
                     /translation="MDYSTLDMLRQKHPAWRMLRSDHAPLIASFLHRVFIAPNVRVMT
                     QASLSEALEDELFGLRESLGSDVFPKPAIDYLNDWATNEKGWLRKFYPHGSDEPHFDL
                     TSAAEKAITWIGTLTERAFVGTESRLLTLFTLLKQLSEGSETDPQARISDLRKRHEEI
                     DAEIARIQAGDIQMLSDTALKDRFQQFIAMARELLSDFREVEHNFRGLDRHVRERIAL
                     WEGGKGVLLEEIMGERDAIADSDQGRSFRAFWDFLMSGSRQEELTQLLERVLALPAVS
                     ELKPDARIRRIHYDWLEAGEHTQRTVALLSQQLRRFLDDQAWLENRRIMDILHSIEAK
                     ALDLRDSPPAGEFMQIDDAGADIELPMERPLYTPPVKPLITDVALQPGDEDLDAAALF
                     SQVVIDKADLLRHIRNALQERLRVTLRELVEARPLQHGLAELIAYLQLAGDAFKVIVD
                     EEITDVILWKPSDQDGGRIRQARLPRVIFVR"
     gene            complement(23776..23851)
                     /locus_tag="VMU21_10465"
     tRNA            complement(23776..23851)
                     /locus_tag="VMU21_10465"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23815..23817),aa:Glu,seq:ctc)
     gene            complement(23987..24060)
                     /locus_tag="VMU21_10470"
     tRNA            complement(23987..24060)
                     /locus_tag="VMU21_10470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24026..24028),aa:Gln,seq:ctg)
     gene            complement(24195..24767)
                     /locus_tag="VMU21_10475"
     CDS             complement(24195..24767)
                     /locus_tag="VMU21_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HUO77985.1"
                     /translation="MKVAVFHGSPRIEGNTEILLNEAIRGIREEGHDIKVFKLNFMNI
                     KPCQDCGGCEKNGTCIINDDMDEIYDAIREADRIILASPIFFFGLSAQSKAMIDRCQS
                     FWCEKYLLRKTIPEGPFGRKGLLLLVGGMKKEIGIQCGEATAKAFFRTVSVPEHRTLS
                     FLGVDAKGAILEHPTALRDAYEAGKALVAL"
     gene            complement(24916..26130)
                     /locus_tag="VMU21_10480"
     CDS             complement(24916..26130)
                     /locus_tag="VMU21_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HUO77986.1"
                     /translation="MKAVELKNGVYWVGVIDWAVRDFHGYATPRGTTYNNYLIIDDEI
                     TLLDTVKYDFWDITIKNITSVIDPSKIRNVVINHIENDHATSIDRIMELAPNATIYIT
                     EKGRKGLERFYDLSRWKVKVVKTGDTLSIGKRTLLFIETPMLHWPDSMMTYIKEEKIL
                     ISQDGFGQHIASSVRFDDEFVTCESMSELDDAIVDYYANILMPFGQVLKTKIAEIQKL
                     GLEIEMIAPDHGIIWRTNPGKVLQAYLDMANGKANLSVPIIYDTMWHSTEKMALPIMQ
                     GIKDEGVECKVIKLRATPMSIAIKEFWKSRGCLVGTPTLNNIMYPTVAEFLTHLRGLR
                     PKNRIMGAFGSYGWGGGAVKEAYEEFKRMGLETFDPGLQVLYRPSLEDETKCYDFGKE
                     FAKKVKEYHQKF"
     gene            complement(26215..26631)
                     /locus_tag="VMU21_10485"
     CDS             complement(26215..26631)
                     /locus_tag="VMU21_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HUO77987.1"
                     /translation="MASKELKDKLNDAIAREIQVSVQYMWQHVMIRGINAESVGGVFK
                     TTAITEMKHAEAIAERLDYLGGVPTTKPTPIAIGKTAKEMLKINKKAEEGAIKLYKGI
                     IALAGKEGDVVTRKLFEAILADEEEHHNIFSTLLED"
     gene            complement(26898..27908)
                     /locus_tag="VMU21_10490"
     CDS             complement(26898..27908)
                     /locus_tag="VMU21_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUO77988.1"
                     /translation="MKLDTTLIRGDADKPAVIFIHGLGMDKRIWESPDTSRILGGKFP
                     IHFLVSTKPAPEILKTDTHRGITKRLFFGKPPKHLATLFHDFREQGYTVIAWSQQRPA
                     AEIAVAVSELREVVSMHHEHCRSGIILIGHSRGGLVARNYLKGDAKGIRGIVTLATPH
                     RGSNMALWVKHVAPLITVIDSLISDSEKGTFTYAVKRVFDFLKSKAVRELLPDSPLFR
                     SCDEGPLQGVYYLSFGGSNPTLFSVYKRIMERTHVRDRERWVVRPQTVFSVPDAFERF
                     IPERLFPDEMKKGKGDGLVSVESSRIPSADEHHTLNVNHAEVLFAERVRHKVLDAVRR
                     FG"
     gene            28021..28317
                     /gene="tusB"
                     /locus_tag="VMU21_10495"
     CDS             28021..28317
                     /gene="tusB"
                     /locus_tag="VMU21_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HUO77989.1"
                     /translation="MKLGVLVSDFRNMDDTLSRLKADKLGVILVSNGVYHAAAKEGGK
                     KSWVLEKSPHLYVLSEDLQSRGFSEAEIDTRVKSITYSDLVDLIFNEYEKIIWI"
     gene            28326..28688
                     /locus_tag="VMU21_10500"
     CDS             28326..28688
                     /locus_tag="VMU21_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HUO77990.1"
                     /translation="MAKLTIGVFSSLVGSLSLDFAVKLSEAAVKKGHSVDFWLSGNAT
                     MLSLKGQRTFKDYSALQKPVQELLATGKFQITACEACAEARGYHKEDTMEGCKRQSMD
                     WYLASSFSADRVVHIGGD"
     gene            28690..29103
                     /locus_tag="VMU21_10505"
     CDS             28690..29103
                     /locus_tag="VMU21_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HUO77991.1"
                     /translation="MATKKLAFIVKSLPYKTEASRLALTHAIASQTVEIYLEDGDMVE
                     PVLAFMGDGVLNCLKNQKAMTLYGVTSLEQHIKNSLLLDLKVMICKEDVERFGLSEDA
                     LIMDAEDIGGETNANIAPFNEIMKEMDSSDHLLFF"
     gene            29113..29358
                     /locus_tag="VMU21_10510"
     CDS             29113..29358
                     /locus_tag="VMU21_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HUO77992.1"
                     /translation="MGELSSATPAQTLDVLGRVCPYPLVLTKKAMEKLPSGAVIKVLC
                     DAPASAEDSIPRYAEKQGFKFESVKNEGYWELYIQKK"
     gene            complement(29468..29647)
                     /locus_tag="VMU21_10515"
     CDS             complement(29468..29647)
                     /locus_tag="VMU21_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO77993.1"
                     /translation="MVTVNAATCEGCEECVNVCPQGVFQMSDGKSDPYQTAECVFCES
                     CLGVCPANAITIKEM"
     gene            complement(29754..30227)
                     /locus_tag="VMU21_10520"
     CDS             complement(29754..30227)
                     /locus_tag="VMU21_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO77994.1"
                     /translation="MEIRKILWPTDFSRNAAQALPYVSSLSEKYQAEVHLIFVVEDVH
                     QYDHFYGDASPTFLKEFQEKIIQKGGQQMEKICQESLASCPLYKKHIVVGDPAKEILK
                     FINEEKVDLVVMATHGHGEERGGEMKHFPFGSVSEKVTRNSPAPVLAINPLRQKK"
     gene            complement(30249..31421)
                     /gene="nrfD"
                     /locus_tag="VMU21_10525"
     CDS             complement(30249..31421)
                     /gene="nrfD"
                     /locus_tag="VMU21_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HUO77995.1"
                     /translation="MLEKALVGSRRYWTWLFFLSAIVGIGFINYLYQFKNGLGITGMS
                     RDVSWGLYIGQFTFLVGVAASAVMLVIPYYLHDFKKFGKIVILGEFLAVASVTMCISF
                     IFVDLGQPMRVLNVILHPTPNSILFWDMVVLSGYLFLNIVIGWVTLSSDRKGVKPPSW
                     IKSLIYLSIPWAFSIHTVTAFLYAGLPGRHFWLSAIMAARFLASAFAGGPALLIMLAL
                     IVRKFTKFDPGKEPIQAVGKIVTYAMIANVFFLGLEFFTAFYSQIPGHTHPFKYLYTG
                     LEGHGKLVPLMWISTILAVVSLLLLVVPQWRRSERILAFACGALFISLWIDKGFGLVV
                     GGFIPNPFEGVTEYWPTLRETSIALGVWALGFLVLTLLYKIAISVREETEGIEIEH"
     gene            complement(31414..32238)
                     /locus_tag="VMU21_10530"
     CDS             complement(31414..32238)
                     /locus_tag="VMU21_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO77996.1"
                     /translation="MSIDRRQFLKIAGISTILGVGGAPVINWLRNDRLEASHVLTDEK
                     ALTGKHWAMVIDMSKFETEEDYRRVIHACHSIHNVPNLDNPKDSIKWIWTDTYDHTFP
                     GQENEYVPEEVKHKPFILLCNHCENPPCVRVCPTKATFKRKDGIVMMDQHRCIGCRFC
                     MAACPFGARSFNYKDPRPSLKEENKEYPTRTIGVVEKCTFCFERLSKGLIPACVEESQ
                     KAAKEAGKEPGMFFGDLEDPSSEVRKVLSTRYSIRRKPELGTQPSVFYIIGGAKNA"
     gene            complement(32235..32639)
                     /gene="dsrJ"
                     /locus_tag="VMU21_10535"
     CDS             complement(32235..32639)
                     /gene="dsrJ"
                     /locus_tag="VMU21_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HUO77997.1"
                     /translation="MKLYNGGKIITGLIIFLGLVTFPFFYNIGKANVRPDPKIDTPAI
                     KELKEKKCVESKEFMRAEHMKLLNDWRDSAVRDGARLYVNTEGKAYTISLQNTCMHCH
                     SNKKKFCDECHTYMAVNPYCWNCHIAPKEKET"
     gene            complement(32626..34251)
                     /locus_tag="VMU21_10540"
     CDS             complement(32626..34251)
                     /locus_tag="VMU21_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUO77998.1"
                     /translation="MAKFTAKPNELSNLDYSPPKTGWMDTPVEIREHMFCHAAKPKEL
                     EIVGFPNAREWRPADEDWKLPENWERLLLEKMKDLLDKYRSFKVFMDICVRCGACADK
                     CHFFIGSGDPKNMPVLRAELLRSVYRRNFTAAGKILRKIAGARDLTVNVLKEWWYYFY
                     QCTECRRCSVFCPYGIDQAEITMMGRELTNLLGLNIGWIAGPIAACYMKGNHLGLEPH
                     TITSSIEFFLDDIETITGIKINPTFNRKGAEILFVTPSGDLFANPGTYTAMGYLMLFH
                     ELGLDYTWSTYASEGGNFGFFTTNEMAKRLNYKIYAEAKRLGVKWILGGECGHMWRVI
                     HQYMDSWNGPADFLEEPVSPITGTKFENAKAAKMVHIAEFTADLIKNGKLKLDPNRND
                     HLKVTFHDSCNTARGMGLLEEPRYIVKNVCNHFYEMPEDTIREKTFCCGSGTGLNASE
                     NMELRLRGGFPRANAVKYVHEHYGVNMVANVCAIDRATLTTSIDYWVPGVGVTGVHEL
                     VGNALVMKGEKERTVDIRLEPLPGKEGTADETV"
     gene            complement(34254..35255)
                     /gene="dsrM"
                     /locus_tag="VMU21_10545"
     CDS             complement(34254..35255)
                     /gene="dsrM"
                     /locus_tag="VMU21_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HUO77999.1"
                     /translation="MGSVFAFFAVIILTLIAFIGVKGASLHFLFGVLIPYVAFGTFLV
                     GIIYRVLFWWGSSPVPFCIPTTCGQEKSLPWIKQNKLESPSSTLDVIGRMALEVLLFR
                     SLFRNLKTQLYDGTKLVYGEAKWLWLAGLAFHWSFLFIVVRHMRFFFEKVPVPIKMIE
                     GLDSFLQIGAPLLYITDAVILAAVTYLFIRRVLLPQVRYISLPADYFPLFLIFSIALS
                     GVLMRYFFKVNVTGIKELAIGLISFSYDPKVLEGTGVIFYIHLFLVSALLAYFPFSKL
                     MHMGGIFLSPTRNMANDSRMKRHINPWNYPVKVHSYEEYEEEFREKMKGAGIPVEKE"
     gene            complement(35300..35824)
                     /locus_tag="VMU21_10550"
     CDS             complement(35300..35824)
                     /locus_tag="VMU21_10550"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HUO78000.1"
                     /translation="MTLWDFLAQKKSALVKRWFDIAMEPYPSDTAGLLKNEKKQFTNP
                     VGYTISHGLENILDKLIDEKGPDFEGMVPFLEGIVRIRAVQDLRPSQALSFVFHLRNA
                     IREELASEAKEFSAEMATLESRIDAIALISFDIFVKCREKVYELKSNEVKNRTFRLLQ
                     MANLVSEGQKEQSD"
     gene            complement(35996..36331)
                     /locus_tag="VMU21_10555"
     CDS             complement(35996..36331)
                     /locus_tag="VMU21_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HUO78001.1"
                     /translation="MPMMEFQGKQYEIDEDGYLINLDDWSKELAAHIAKTEDIEMTDA
                     HWEVVNFLRDYYQKYQIAPMIKILVKELGKVMGPEKGNTKYLYELYPGGPAKQACKIA
                     GLPKPTGCV"
     gene            complement(36514..36801)
                     /locus_tag="VMU21_10560"
     CDS             complement(36514..36801)
                     /locus_tag="VMU21_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78002.1"
                     /translation="MPETIRTDTGEFIGGKCECGAVYACDPTGHNVGEAYLDALIYAS
                     GGDWNKVQSLDAESFHEAVRNYDMRTHRMWEIRDMRRDYTGKIILIKVGPK"
     gene            complement(36898..37575)
                     /locus_tag="VMU21_10565"
     CDS             complement(36898..37575)
                     /locus_tag="VMU21_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78003.1"
                     /translation="MRYVSQGIESLRVGLLKEEIRDALFEEDFAKVVQRALENKKVFS
                     ILISFTYDKESVLCWRAIEAMGIAAGAVAERDPSTVRNIVQRLLWSMGEESGGIGWSA
                     AEMLGEIVINTPETCRDIPPILLSFKDEESFLPGVLWAIGRIAGAGMNNGEGAEELVI
                     QSLSHRSPLVRGLALSAVSTFNIMSDKVNFLIRDGERFRVYEDRELVERTVGDVAQRV
                     LDEQSYT"
     gene            complement(37683..39086)
                     /locus_tag="VMU21_10570"
     CDS             complement(37683..39086)
                     /locus_tag="VMU21_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HUO78004.1"
                     /translation="MLNCPRLVIAGLKGGSGKTTIALGLVAALKSHKGLRVIPYKKGP
                     DYIDAGWLAHAAGKPCYNLDPFLMPREKALESFVSHFDGDVAVIEGNRGLYDGVDEEG
                     SYSTAEVSKLLKSPVILVIDCTKMTRTAAALVLGCMQLDTQVSIKGVVLNQISGGRHE
                     AVVRKSIEKYCALPVLGAVPRLRSGDLPERHMGLTPYQEHDDTDRAISVVEGMAIKYL
                     DIEGILKVANEAEPLPDKDRAGFKWNADERNVKIGVIRDTAFQFYYPENFEELEKKGA
                     LIVEINSLEDDLLPDIHALYIGGGFPETHALALARNVKFKNALRSAIEEGLPVYAECG
                     GLMFLGDALVVNGEKYPMAGVFPLMFEMRTRPQAHGYTVVEVEQQNPFYPVGTIMHGH
                     EFHYSAVLNPPGDDMLPTAFRMRRGNGIGGGVDGLLYKNVFATYTHLHAYGAPDWVEA
                     MVRLAKKFSKTRTSLSA"
     gene            complement(39242..>40912)
                     /locus_tag="VMU21_10575"
     CDS             complement(39242..>40912)
                     /locus_tag="VMU21_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUO78005.1"
                     /translation="LTTPLIRREGMLQRASWEEALDHIAESLRWIKEKKGAERIGAIG
                     SPRFSNEDNYMLQKFMRQVLESHNIDSIARFGFAKMQEAVEEVFGLTALSVEQTAPIG
                     AGVVMVIESELDTTHPVWSLNFIRAKHQGTKLIAVQPRRTKLAIHADQWLSVNPSTSE
                     ALLKGIIHLALAQGTHLKKDAPSIPHFNELAASVSSFTPSYVASATGISEKEVVDTAS
                     LFLESSPRVIAMSLGANENEKGKNTVLTAANLLMLTGEGPRGLQIPCEYNNSFGMWKM
                     GVAPDRLPGFQKLTPGGKDAFQMLYEPDRINALFVMGEDPVVNFPNASRVKQMISALD
                     FLVVQDEWLTETAKLAHVVLPTAVWVETDGTYVNAADISQMTRKMLTPPGEAQPAWQA
                     LNELARLFDPSVGKNDFHSLREEIARIEVDAYADLPRLVPTEFRQLESPDEKYPILMI
                     TVTLMQHSGTLSLASRNLRKVKPEGFIEVSEKDAVRYGIQNNRFVTVTSRHGSIVIKA
                     HITSDAKPGVAYVPIHFPLANVNNLTGIAHDGAVPSCAVRIEPISESR"
BASE COUNT        10120 a        10535 c         9403 g        10854 t
ORIGIN      
        1 aaaaaccgta tgaagaattg catcgtcaga caactcatta aggctgtaag cattggcgag
       61 aaatctcaat cctgagagtt tctcctgcaa gaacgactca atagaccgct gtgagtcttc
      121 aatctgccac ctgagatgag cccggaattc atctttaagg atattgaaaa cccaaaaata
      181 gctcatcata gcaatcccga tgagggggag agctgcgaga gtgcctatat agaagaataa
      241 acgtaactta agagacttgt aacggtcttt tgttatcgcg ttagcaatat tcgtgaacgg
      301 ccttaaaatg ctttgcataa agggagtgag agccgggtct ccttttctgt tcattatagc
      361 acgttttcgt ttcatgcaaa acctgaagcc tggcctgttt tgtgctatta tatttttact
      421 ggactttcgt aatacactct ggggcatgca cggaaaaagg agaaaccatg aagctgtggc
      481 aacatatttc aggcctccta aaagccgaag agacacacgg aaaaaagaaa gatgagggca
      541 gtgtggtcct gacacagaag ttctgtgcat ttcagcggct tttgtcgagc aataatgccg
      601 cactcgaact catggccgat atgggagaaa agctctccgg ggatttcctc tttgaccgcc
      661 actatgtgga ctccgccgtg agcgctgttt cggaaagggt caaagacctt gtcgaaaatc
      721 tcagaatcat ctcaaaagga aagtaccagg ctttggatga gaggttcgat gaaattcaat
      781 ccgggattca gaagattctc acccagaaga aagaaattgc cgagagcccc tatacgcttt
      841 ctttcggtga gattaccagg gaaaaggcgg agatggtcgg ttcgaaggtg gccaacatat
      901 gtgagataag aaaccgcctt gcacttcctg ttccagacgg attcggcatt accgcctttg
      961 ccttcaagag gttcatggaa caaaacgatt tcttcggaaa gataaaggag aaactcgatg
     1021 atctgtcgat tgacgatctt gaggcattga atgcgacgag cagggaaata caggacctca
     1081 ttgcgggcgg ggaaatcccc tctgatctcg aggcagctat agccgcgtca tacctaagcc
     1141 tttgcgaaag agaaggctcc agggtaaagg tttccgttcg gagcagcgcc ctccaggagg
     1201 acggagagtt cagttttgcg ggacagtaca gcacgttcct gaatgtcccg ttcgaatcgc
     1261 tcttcgaaaa atacaagtgc gtcatggcga gtctcttcaa cccgcgggcg ctgttttatg
     1321 ttaagaccaa gggctttcag gaatcggaca tggtgatgtc cgtcggtgtg ctcactatgg
     1381 tcgacgcagg ggcagcgggg gtcgtgtatt ccgccgaccc gaatgatcca gaaagggacg
     1441 tgatcatcat cagcgcggtc aagggtctgg gagtgggcct tgttgatggc acgataacgc
     1501 cggaaatata tcttgtttca cgctcgggag gcgagatcat cgaaaagagc ttgtcaaaac
     1561 agtcgcacat gctcgcttgt aactccacgg gaggtctcga agagatcgtc cttgatgaga
     1621 ggcatgacgg aaaagccgcc gtcgccgatg aggagatcaa gaggctggct cgttatgctc
     1681 tctctctcga aacccacttc cagggttgcc aggacattga atgggcaatc gacagacaca
     1741 agggtctttg cattttgcag tcgaggcccc tgcgcgtatt ggccagaaag gcatctgtag
     1801 ctgtgccgac gcaggtgaag ggatataccg ttctcattga caaaggtgct atcgcattca
     1861 agggaatcgg tttcggaaag gcctatatag ttagacagga tgaagatttg gaagatttcc
     1921 ccgaaggtgc ggtactcgtg gccaaacaca cgtccccaaa gttcgttgcg gtcatgaaca
     1981 gggctagcgc tattgtcacc gatgttgggt cagcgacggg ccatatggcg tcgttggcga
     2041 gggagtttca gattgccgca ttactggata cggaggttgc aacatcgctc attcagcagg
     2101 gtgaggaaat aacagtggac gcgatcaact gcaacatata taagggacac gttgcggagc
     2161 ttgaagagtt cgcggcgaag agggaaaacc ttttcaagga gaccaggatt tttaaaacac
     2221 tggaggacgt actgaaactg atatctcctc tctatctggt gaacccggac gataaggcat
     2281 ttaaagccga atcctgccgg acatatcacg acatcacgcg tttctgccac gaaatggcga
     2341 tatatgagtt gtttgacgtc actgggatat ccgccgacga agtgggggcg ataaggcttg
     2401 tggcaggcat acccgccgat atctatctca tcgaccttgg cgggggcacc agcgggaaga
     2461 tattggagga tacgaagaat cttctcctga aggatatcag ctccataccg ttcaatgcct
     2521 tcctgagggg tctgacggcc atgaagtggc ctgagccgag gccggcagat gctaagggat
     2581 ttctcggcat gctcgcgacg acggcttcga ttcccgaaga agaacttaaa cggactgcag
     2641 agaagagctt ttcgatcatc tccggagatt acatgaattt cgccataaga ctcggttatc
     2701 acctgtcgac cgtagaagct tatgcggggg agaacatcaa tgacaactat ataaaattca
     2761 ccttcaaggg tggcggggcg atcatcgacc ggagactgag gaggatcagg ctgataacgg
     2821 aaatactcag gagactggat ttcagcgtaa aagttgtgga ggatgttgtg aatgcttccc
     2881 tgatgaaata caggaggtct tcaacagaaa aaacgctcga aatcctcgga aggcttaccg
     2941 tttatacgaa acagctcgac atggtgatgt acaacgacgc tataacggat gtttacatcg
     3001 aggagttctg ttcggcctac gtcaccggtc actgagatgg ttctttacct aagctctccc
     3061 tgagcatctt gttctccctc tgggtcctca gccacttgat cgccttttcg atggtaaaga
     3121 ggatctgttc ctttctgaat ggtttcgtta tgaaatcgaa ggcgttcttt tccatcgccg
     3181 cgtcggcagc ctcggaagtg gcgaaggcgg tgatgatgat tatcgggata tctgcatcga
     3241 attccctgac ggtcttgaga agctctatac cgtcgagacc gggcatcttc agatccgtga
     3301 tgaggagatc gaatcccccc ttccttgcca gtcccagcgc ctctaaagga ttgttggtag
     3361 tcgtcacctc gtaagccgtt ttttctctta cgatcatttg cagaagccgt agcatgtccg
     3421 gctcgtcgtc cacgatcaat agtttctcag gcatctttcc cctccctgtc tattttacca
     3481 caggcagtgt tatgatgaat tttgttccag gtgcattcga ctcgtctctt gtccttgttt
     3541 caaagcttat cgtgccgccg tgcttgtcta taataccata cgctatcgag agccccaaac
     3601 ccgttccttc tcccaccttc ttggtcgtga aaaaaggatc gaatattttg gcacgttccc
     3661 tttgcttgat tcccggaccg gtgtccgcga tctgtatctc tacacgtttc ccttcgtcta
     3721 cggcccttga ggcaacggtg agcgtaccgc cgcctttcat ttcatgaatg gcattattaa
     3781 tgatattaaa aaaaacctgc tgcagttccc cgaaactccc tcttattatt ggcagacttc
     3841 cggacaggtc gaggttgaca gaaatacggt tagtcgtcag cacactttcg acgacggcga
     3901 gaacagaccg gatattcttg ttaatgtcta cgtcctcttc ccggtattct gtgtatctcg
     3961 cgaaaccgag aaggttttcg acgatcctct ttgcgttctg tccctgcctc tctacggcct
     4021 tgagcagttc gtagaattca gaatcttgag gaaccttttc gagcatgagg tccgtgaaac
     4081 ccagaattat cgtcagcggg ttattgatct cgtgggcgat acctgcggca agtgtaccaa
     4141 gagacgcaag cttttcggtg tgggccatct gctgttcgat cttctttttc tgcgcgattt
     4201 catgtgcaac cttcattttt tctatcagtt tactgaggtc aacaggcttt tccaggaagt
     4261 cgacggcgcc ttctttcatc gcatcgacac ttttctcgac tgttgcatgg cccgtaagga
     4321 cgatgacctg catgtccgga ttatccttct ttatccgctt gagggtttcg attccgtcga
     4381 tgtcgggcat cgagagatca agaacgatga catcgaaggc cttgtccttt gcactagcga
     4441 ttgcggtgtt cccatttgta gcggtatcta tgacgaaacc gcgcgcctca agccttttcg
     4501 acaggacatt aaggaagtct acctcgtcat cgacaagcaa tatgttcata ggggtatctg
     4561 cttccatctg ccctccccga taagatcaaa aaaacagttg atctgcttcc agaattatac
     4621 accgtacgtt aaaaagtgag aagaggggaa ccaatgcctt cccctcttct cactttttca
     4681 taggtaatca gcaattattc agacgtcaac ttcaccacat agaccgagca cggggcatag
     4741 gcaactacct ttgaagcaac acttccgagt ttaaaacggg aagatttgct gagacctctg
     4801 ctgccgataa cgatcaggtc catcttttct ttttctgcaa cgtcaatgat ggcgtcggta
     4861 atggaagtgg cgaccacatt ggatctgcaa tcggcagaga ctttctcggc ctcgagcatc
     4921 cttctcgcct cctgtatgat tgattctcct cgactttcga gccgctgtgt caccctgtcc
     4981 ctttcaccgg ctgaaatttc aaggtctatg tccgtaaagt ccgtaacgac attaagaagg
     5041 catatttcgg caccccgtgt ttttgcgaac tctacagcta ccttcagagc ttctctcgaa
     5101 tatcttgatc cgtcaacagg caccagtatt ttcatattgt cctccttaga cttccttctt
     5161 tgctttggcc ttcttcatgc tcagccctaa cccctcaaat acgtagaata ttgagaatcc
     5221 ccaccacatg gtataggcga cataaaagac cagcagaaat gtcatgagaa cgatatgatc
     5281 tttcacttca acggccttaa cgttgtaaaa gttatatgat ggttcaatcg ccttcttaac
     5341 tacatcagtg attattttgg cctcttccat cttcttttct ttctcgaatt gtttgcttat
     5401 ctttggcaag gtattgttcc actgcctgaa catctgcttc tcatcatcgt atgcatactt
     5461 cgccttgatc ttgtctcctt cattcttaaa catggtatcc gcatcttgta atgctgcttc
     5521 aagaacctgc cccaggtcac cttcgatcct taattcagag cctttcaatt ctgttttagc
     5581 acccgcaact cccaacactt tagccgacct ttcagccctt ttctcagctt ctcctggttt
     5641 atcctcaggc ttgtccatcc ttatcgttgc actaaacatc ctgcccttaa acttctcact
     5701 gctctttaca accttgggga taaaataaga tgagcccttg gcgagcctgt taaacatttc
     5761 gtcagaccac tccaggccgt ttactttctt ttcacctatc gaaaaaagag gtgaaaatat
     5821 caggaataat accacgagga acgaaatagc caagactatg cccaggctta gatgttttcc
     5881 atgtcttacc ggcatttcta cccctcctct ctcaatactt ttatatttcc gaaaaacttt
     5941 gcaaagagcc agacaccaaa gataccgatc attatccaga acacccagaa cccaacggcc
     6001 tctattccat ctgccatatt ctttgatatc ttcacataac cgagatctcc caatttcttg
     6061 ggaagaacgg tgatcctgtt ggcaaaccct gcaaggatag tcaaggcata aaatgctctg
     6121 atatggatac cctttaccac cttcgtcgta agtgctccca cctgaatacc aacgagcgaa
     6181 cctaaaagca ttcccattgc gagagtatag aacacatatc cgtaaatagc atattgtgtt
     6241 atagaggcat aacctgcggt aaagataatc tgcaggatat cggtgcctac ggttgtcatg
     6301 gtggagatgc cgaatgtata aacgaacatc gggaaggtaa caaaaccgcc gccgactccc
     6361 atgatggcag caagaaaacc tacaatagat ccgcctatag ccatgatcac acctgatatc
     6421 cttttgcctc cgggaacgat gtcctcatca aacttaatca tgggggggat ttttaatgcc
     6481 tgtaacttta ttgctacacc tgtcgtggtc ttaggtgcgc ctccgccatg tgcgcctcca
     6541 ccatgtgcgc ctgcatcttc actgtgcttt cttaatttaa ggtaatcgac caaggcatag
     6601 gtgcccagga agccaagtat caccgcatag accacgctta taaatacatc gctcatcgct
     6661 gggtctatat tatagagcgt cctgttaata agacctccca cagtagcgcc tattgcggag
     6721 ccgcaaacaa atgccaccgc taaacctaaa gatacattcc ccagtttctt atggacaacc
     6781 gttcccatta ttgcctttgc aaatatgtgg aaagtatcgg taccgactgc catgattcct
     6841 ttcacaccgg ctgccatgag ggcaggcgtt ataatgaacc ctcctccggc accaatacat
     6901 cctgtgatca gacccgcacc aagaccaatg gctattgata cgataaaaat ggccgttgaa
     6961 tagtgtgcgg gagaatatgc ttccttccct cctatgacat ctgctgcatc taaaagtgaa
     7021 atagccacta taggcaacaa taagatgaaa aggatcagaa gtttctttcg actcttgaca
     7081 atgctcattg aaacttcgta atcccactcc gcatgagcta tagacgccat tttgagaaat
     7141 tcgaacatcc ttctcggggc gttcatttaa gtacctcctg ttattttatt ttgtcgatct
     7201 tcacttgatt ctctcgaatt ccgtctgaat cttatacttt acaccactcc cgataaggct
     7261 cgtataggct atagggattc cgtcagaacc gataagaagc ctgtgcaaga gcaagccggg
     7321 agacccttcc ttcacttcca ggatatgggc tgcatcgcct gttatctctg taacctctat
     7381 ggtctgcagg actttggata tccttctcaa agtcttttcc tgtatcagat cataaaaaga
     7441 agtgctgcat aaatcttccc tttcgatacc cggaaacagg gcctcgggga taaacgactc
     7501 ttcgagatag gcaggctcgt cttcgacaac ccttttgcag aggacacggt agaagtcccc
     7561 ttccaccttt agatacctct taatcttctc tgtcggctcc tttatacctt tttccatgag
     7621 ctctttcctt gtcttcaccc cctctccgaa catatcttcc gtcaatctgg ttctcatcgt
     7681 catgcctgaa tgagcaattg aacgagtcac aaaagtgcct ttaccctgtt gtcttcttag
     7741 atacccatcc ctcaccaact ctgctatcgc tattctgatc gttgccttac taacgttata
     7801 agctctgcaa agttcgtcct ctgaaggcag ccggacgccg caataccact caccactttc
     7861 gatctttgct ttgaaaatcg tgtaaatctg cacatagagt ttctgctcgc tgtccctgtc
     7921 aactgttttt tctatctgca tatatttagt tctttatgtc tttataaagt tattcctcaa
     7981 acaaaaaaaa tatacactct ccgacagctc ttttaccagc attcaaacgg cgtaatcggc
     8041 aaaaactgac aaaagagata agtcgaaata agcgttacaa gacaagcgag caacacatat
     8101 ttcagctcca tgcgaagatc tttatccatc tgaacggtcc cccgcaccgg ctgcaccgcc
     8161 tcttcgctgc cttcgcgcga aacttcgacg ctgcgccatt cgtcggcaat catttccttt
     8221 acaaggtcat attcgcccga ctcggcgaaa gttatcgata ccatcatgtg ctcgaacatc
     8281 ttcatcaacc ttttcatggc gaaccctcct ctaaggagat ccggtattta caaagaatct
     8341 tgacaatcta gaagcaagat acataccgca gatttaactc attgataaaa aaggataaat
     8401 aatttaagtt gggggactta tgcatcacaa agatgcatag aggatcttgt tctcttgtaa
     8461 tatcaagaaa ataagggaaa cttatgcaac aatttatagc agagacctat aatgttgcta
     8521 tcttaaactg ttttattttt ctctggagct gctgacggta aataccgagt tcatcagcgg
     8581 cctttgatat gtttccccgg gacttttgca acgcggacca tatcaattcc ctttcgagat
     8641 tctcaagggc attcttcagg ttcctgctgg atgctgcctt tctgggagcg gcctgcgcaa
     8701 ggcaagaagg aaggtctgaa ggcatgatga caggactgtt ggcaaaggag atagcgcgtt
     8761 cgataatatt ttcaagttcc ctcgcatttc cgggatagga gtggtcgagc aaaatgctca
     8821 acgcgtcatc cgatatgcct cttatcagga aatcttttct cttggtcgca tgcttcttta
     8881 cgaaatgttc tgcgagcagc ggcacatcct caattctgtc tctcagcggc gggagatgga
     8941 tattaatgac gtttagccgg taaaagaggt cctccctaaa gatgcctttt tgacaatcgt
     9001 tcttcaggtt cctgctcgta gccgcaataa tcctcacatc aatcttctgg ggacgcgatc
     9061 ctccgatccg catgatctcg ccttcctgaa tcactctcag tatctttacc tgaaagagcg
     9121 gggagacgtc accgatctca tcgaaaagaa cagtgcctcc gtctgcaagt tcgagcagcc
     9181 cctttttcgt gtaattcgca cccgtgaaag cccctttttc gaaaccgaac agctccgatt
     9241 cgaggagcgt gtccggtatg gcgctgcagt tgatcgtcat gaaggccctt ccgctgcgag
     9301 gactccaccg atgaattgcc cgcgcaacaa gctctttacc ggtgccgctc gctcctgtga
     9361 tgagaacatt actctcgctg acagcggcct tcctgattag accaaagacc tcctgcatcg
     9421 gcgggcttat acctatgatc tcgtcaaaac catgcttgtg gtcgagaatc ttctttccgt
     9481 gacccttgat cacatcaccc aggctggcaa gcgtaagggg tttaccgagg aaatcagagg
     9541 cgccgagttt catcgcttcg acagccaatc taacgtccgg ggccgacaag gcgataacag
     9601 gcatgcgctg acgcagctca atgagtttct gctgccatcc ttgactgtcg aggtctagta
     9661 aggcgatgtc gtaggcaaag gaaacatctt tctgtttctg atgctctttc tggggaacaa
     9721 agccatgtga cttgagcatc gcatagatct tttttgcatc atcggagata accaggatgt
     9781 taagcatacg gatattttaa tacactgtca gcacaaaaac ataaaaaaag gacgtccgat
     9841 agaaactaat ggaaaccatg cgtcggatat agtttttgcg tttaatttaa atagaacgga
     9901 aacatagcct tcccctttat cagaagtttc ggtattgtac cacctcctaa gtatagagaa
     9961 aaagagaggg atgcagggat agatattctt tcaatgtttc ttgaaaggga ggcggataac
    10021 acatcagaac cttctttttc tttcatctgc aacctccctt taagcaatat gcgtattccg
    10081 aaccatcgcg accagtcatt ccggagtaac gcgaccactg attcctggct gagcgcgacc
    10141 acttttaggg tggttccgga acagttagtc gctatgagcg gaatcggcac aacaaattga
    10201 atgacctatt tttgcataaa cctgccgtca ggtgtaaagc aaattctaaa cttttatctt
    10261 atagacataa attttaatga tataagaatt atagtttgac atttagataa aaacgtttaa
    10321 gataatagaa agggagaggc gggaaggtat ctaatagcta acagatctaa tggtggtgcg
    10381 tgtcttggtt ccttttcttc attgcggttg caggaatgtg tgcttcgaaa aatatatcca
    10441 ggaatatact tgtcaaggca aaaactactt aacatagaga ggaggtagtt atgtcagggt
    10501 tacaatggtt tatggtgctg tttggatggt tttcgctcgt ggttttcgtg gtgggagctg
    10561 cgtataaggc cgtaaagttt tctaaaatgc cgttaaacct gcgctgggag gtttatccgg
    10621 ttccccatga agagggggag aaacgtcatt atggcggctc ttacatggag gagatggact
    10681 gggctaaaaa gcccttgccc agctcacttg caggagagat tgtggagatg ggctctgaga
    10741 tattcttcct gaaaaaggtg agagtgtata atccttataa cctgtggttc ttttcgatgg
    10801 cgatgcactg gggtatttat ttacttttcg gctgggtctt cctcctggta ataggggcta
    10861 ttttaaaggt tgatgcctta tcacagatta ccggcatcgt cggcatagtt tcatttgtcc
    10921 tgggagcctt cggttgcctg actcttataa tcaagagggc aacaaacagc gagcttaagc
    10981 tttacacgga acctgtagag tatttcaacc tccttttgct gtttgcaatc tttgtgacag
    11041 gcattatgtc ctggcgtgtg gacccttcct ttgccggctc cagggactac gtaggaggtg
    11101 ccatcttttt taaccccact cccgtaccct cgatcgtact gttgaacttc cttctttttg
    11161 agatcttctt aatctatatg cccttttcga aatatcttca ttacctggca aaatatttca
    11221 atttccacaa gagcttatgg gatgatgcat tcaagcagaa aggctcgccc aaagacttaa
    11281 agaacatcga acaggtcggc tacactgtac actgggcagg tccgcatgcg tctcctgatg
    11341 agacctggct cgaaaatgca cagaagtggc ttttagaaaa gaaggaggaa aagaaatgaa
    11401 aaaccaggtt agttttagag atatagccag gcttacggat gagccactta ttaacctcga
    11461 agtgaaagat ttgatggaac ttcctgtgga agagcctcct atggagcctc ccaaagaaaa
    11521 atggcttgaa tcgtatgatt tttctcttga cggacataca ccttttgatt atccgaaacc
    11581 aaagactcag gaggagaagg aaaaattagt tttcggtttt atgagcggac ttgaaaaact
    11641 ttttaaccgt gaagctaact ggacattcct taagatcttc aagctctcga tggattactg
    11701 tgtgaaatgc cagacctgtt ccgaggcatg tcatacatat cttgcaagcg gcaggcagga
    11761 gatatacaga cctacctaca ggtcggagat attgaggaag ttatacaaga ggtattttac
    11821 cccaagtgga aagctcctta agtcttttgt aggcgctgat gtagaggcca attacaagac
    11881 agtatggagg cttttagaat tggcttaccg ttgcaatgtc tgcagaaggt gtgcctctta
    11941 ttgtcccgta ggagtagaca acgccctgat agcaagggag ataagaaagg tcttcagcca
    12001 ggagcttggg atcgcgccat tagaattgtt gaggaagggg tcgagacagc accttgcgaa
    12061 aggttctact acaggaatga cccctgcggc atgcaaggct actatagagt ttctggaaga
    12121 ggatatggaa gaaagaaccg gcaggaagat taagattcct atagacaaga aaggagctga
    12181 catccttttg atgcagaatg caggagagtt tcttgcatgg cccgataacc ctgaagcgtt
    12241 tgcgatactc ttcgatgctg cgggcattaa ctatacgtta agcagtgaga tgtgcggata
    12301 tgacgcggtg aactatggtg tatggtttga cgatatagag gcggcgagag taggcttaaa
    12361 gcacctgtcg atagcaaagg acctgggtgt taagaagata gtggtagggg aatgcgggca
    12421 tgcccataag gcgatgtgcg tactcctcga ccgcatagcg ccgggagact ttgcccttcc
    12481 acttcttccg cgtgaaagct gtctgccgct cttctgggac atagtaaaaa gtggacgagt
    12541 taaattagac cctagcagga ataacttccc taccgtcctg catgattcct gtaatgtggt
    12601 gaggctgatg ggaatcgtga aaccgcagcg caatacgtta agtgctattt gtgcccagcc
    12661 ttacagagag atgaacccgc atgggccgat gaactattgc tgcggcggcg gcagcgggtt
    12721 tgcgatcatg aattccctca atttcccgca gttcaggaag aaggtgactg agcggatgaa
    12781 ggtaaaacag atactcgagg cattcaatga tgtccttgat ccaagcatta ataagatggt
    12841 tgttgctcca tgctctaact gtaagggcgc cctgagggat gcccttcatt actacggact
    12901 ctgggagcac cacaggatac tctattcggg tctggtggag ctcatagtca atgccatggt
    12961 cgatatcccg ccgtttttga agtgggagtg ggaagagtaa gaggcataat tctgtatagg
    13021 gcaaatcgac cggtcattcc gaagcaggtt gtccactgat tccggggcat tccgtacggc
    13081 gattccgctc tgaagccgtc cactttatta gtgaggccgg aacaggtggc cggtatggat
    13141 cggaacgaag aaaattctat ctgaacgcat agtcttagct gggtgaaatc cgtcattcta
    13201 tcttcttatc ataattagag gaggagttca tcaggccgga tgtagggaca agaaactccc
    13261 ctatggccga ttcaagttcg atcaggttgc aagcacacat gccgatgtgg tcatagcgcc
    13321 ctgtcataac tgccatacac agtttgagca attgtgcgag aaataccatg gcagctatga
    13381 gacgtggcac ttgtgggatc tcattataaa ggcgcttgtc agatgatctc ttctgccggg
    13441 cagaggaatc gcgaatcgct gtggttagac gtccagtcta actattgtat gtatcaggta
    13501 cagcctgcgg tgaaaggaat gccggcatat ttgcatgctt tcattactgg ttgctctttg
    13561 aacaaatcgt agagagattt gacggtgtat tttccgtccc caagcctctt gttgaggttt
    13621 ttgacaataa atttgggatg agggcagttc tggtaggtta tgtaatacca gcgcacgaaa
    13681 ttgataacat cccagtgatc ttttgaaagt tcaatcccat cttctttgct caagtaaatc
    13741 gctaattcct cgcaccaatc ttcggcacat ttcagattac cgtcttcgtc gacttcaatt
    13801 gttttgcctt gaacttcgat atatcgtgtc gccaagggtt ccatcgttat cctccctata
    13861 gctataatta atatataggg tactaattct atatataaca caattcgtta ctaaatgcaa
    13921 tggcatatac atgttgcaat cattttcgac gtagctcctt agattcatca aaacaagatc
    13981 aatactaatt ctgtataatt cgtataacac aatttgttac taaatgcaat ggcgtgtcag
    14041 ggttgttgtg aatcgccttt atagccggga gcctcataag aagtcagaca atttgcgaga
    14101 aagggcgggc tataataatg gaaaacttct tccttttaaa ccgttacagg gcgtttttga
    14161 gtgaagtcaa aaaatatgta tgtaatattt ctatttggag ttataataat tgttagagta
    14221 cttatgaaaa agaattgttg ggaatacagc aggtgtggaa gggaacgagg cggcaggaac
    14281 gaaaaggaac ttgggatatg tccagctgca gcggaggaaa ggctcaatgg tgttcatggc
    14341 ggcataaatg caggcagagc ttgctgggca gttgctcagg tattttgtga ctgtactcgg
    14401 tgcaattttt ttcagttagt aaaagaagaa gaaggtacca attacgaaac atctctggcc
    14461 ttgcttaaga agctaactac tagcaaataa accagcacgc ctctggatat agactaaatg
    14521 taagatgatg gaccaatccc catccaagcc gttggacaaa tatggtttaa cgcgcatagt
    14581 aatcgcggtg cttattggat tcattgtctt atgggctttg gccaatctca ctgctgcagc
    14641 aggcggcgcc aagataccag atgacctcgg tgtcatacat gggattacag agaagacttc
    14701 gtatcaaaca cgaaagtcac ttcgtgccaa acgccagctt cgccctttgg atgccttaga
    14761 atctggaggg cacgtggctg atactccccg aggaacttat tttttcattt tttaccgtga
    14821 tttgagggca catgagggca gaatggttaa ggagttgggt gcccagcgtg caaaccggta
    14881 cagagagggc aatgcctttt tcgaggttca ccttctaaag gttggaccgc ccattatttt
    14941 aggtttcgct tcagaaagcg acgccttgcg agttagcagc ccatcaacac agattcaaaa
    15001 gatatctatc gcatctgccc cgtgggaaaa gatgacctcg ctactatccc ttccagcgga
    15061 tcgtatagtc tctgcagaaa cgcgtaccgt tagtccttca gaaagagaat ggatgtatat
    15121 tttagactgt gatatccgat gattagcagt gcgtagtccg gtcgatccac cggctcgccc
    15181 aaaaccgtgg gctcccggtg gcacactttt gcgagctatc tcaggcaaaa cggggtagac
    15241 ctccacacga tttcaaaact cttaggtcac aaggatatca gaatgactca gagatattct
    15301 cttctgtcgg ttgagaatct aagggaagct atctctgtcc tcgacaaatc aaggtgttac
    15361 aattttgtta tatttttatt ggcggtccca acgggattcg aacccgtgtt ttagccttga
    15421 gagccctaaa tgggccatct tgctaagtgg gccttctacc tgtattttta tgaattatac
    15481 actattttca agaagtttta tcattccatc ctttacggta ttttcaggca ttttacgccc
    15541 ctttggaaat ttttggctac attttggcta cactgaccga ggggcaaatg tcttggtgtt
    15601 gcacgtgcca gcatgacttt tccgccagcc cagaagatct tcagtcagtc aaaatggcta
    15661 cattatcgct acagtgtctg acagaaaaat atttcgactc gcgccgtcaa tttgtcatag
    15721 cagacgtgac cataagtcaa gaatcgcaac tcgtgggcgt tttcgttttg tcctatcttc
    15781 aaagccaaga cagctcaaat tcttctcact ccaatcaagg atggaggtta tcgaaagctc
    15841 gtcgagaata ttgttttttt ccaccacact cttactttaa tcctaattgc tgtggaggaa
    15901 aattcattgc ctcacaggaa gctctcaggg gcgcctctct aatcaaaaac ctcgaaaccc
    15961 ttaaggtagt cgtcaaatcc tataggagct tctatgccca gttctttgag cagatctatg
    16021 gcctcgctta tggatatatc gagcttcttg gccaaaccgg taagcgaaag cttcccctct
    16081 ttgtagctct taagcatgag gaaatcccac ccgtattcga gcagctcgcg ggctatggcc
    16141 gatttatcct tgcgctcact catagaaagc tcctcaagcc tttccatatc cctgtctttt
    16201 agtctcaggc ttatcacgcc catcacttca ctcctttcag cctttctgtt gcatcctcga
    16261 ttattcgctt gttgtacctg ccgtagccgg aaagtctttc taatttggca agagccatgg
    16321 gaagctccag cttcttgcga gtgcggagat tcaatagaaa atgaaccgcc gtcgcaaact
    16381 gtagccccag aactttgcac gcctttatgg caggcccgtc gtcgaccgcg aggggacagc
    16441 cgatgcttct tgaaagccat aacgcctggg cctctgcggt ttcgatcctg aagtctatct
    16501 gaagctttct gatcacctcc tgatttccga ctttttccac cttgatcttt ttctccataa
    16561 taagagcctt gattgctgac gcgtcagggc tctccttcaa aaggcattct tctttcacct
    16621 tctggggaat aatgatgtga atatcttccg tgacctctct caaaaggtcg attttcgcca
    16681 gaagaattag agtggatgaa tcgaaaacca tcctcatgtt tacaatatac tacatatgta
    16741 atcatttttc aaggctgacc gaggggttga ttggctaagt tgccgatact tatggataat
    16801 tcaagaccgg aaaataccgc accgatcttt gtcagattct tgattgttgg tcaagcgata
    16861 ccagcgcggt cacaacccag ccgaacctga tcctttcctg ttcaagcctg atagatgagc
    16921 ctattcgatt atctcgcaaa tcatcataca gtgcgcgctc ttcctggcta agccgaacaa
    16981 gatcacggct caagggctgc ggctcgatca cccacagcaa gtcatgagcc atcaatgtct
    17041 cacggtccat taggaaggac tgcgcatgag gaaagtatgc cctcaactga tcaagaattg
    17101 caaagccgtg ggtatcgatg tcgccccagt agtagatagc acattgatgt aaccacttcg
    17161 cttgcgccag catctcgaat ccgtaccccg cgccgaaaat gaccatgctg tcagacagat
    17221 ttggaaaggc gagaaaattg acttcgtttt cggtgatgaa tacccggccg accctcgaat
    17281 cgagttgact gaatgcgtct tgagtgacag tgatatcctg gtcggcactg cccggcaata
    17341 gggcaagcgc cgggtccagc acgcggaaac ggatgcgcaa aggcttatcc aggaatccat
    17401 aacgtcggca gaattggttt actccaccgg tttcagtatc gacagtttca ggcctcagaa
    17461 caaggtccaa caacccactc aatacagcgc gatgcgcttc gatgaacttt gtatgcacgc
    17521 cctggatatc gatctgacgc aaatataaac ccggacgcgg atgggctctc atccagtcga
    17581 tgatgttcag cagatgccgc cagtcctcag ccaactccag gaccttaagc ggacgtctag
    17641 aaagccaggg cagtatggcc ggctgccgtt ctctggtgag ctcaacaagt tctccgaatc
    17701 tcctggcatc tcgacctttg ccgattagag caagggcatc atcgggagtg tcaatccata
    17761 tttcatcagg aacggcattg ctgcctatga catgatgccg gacttcgcgc atgactatgc
    17821 ggtaatgcct aacacttagc agttcggcaa tccatgtccg cacatcctca aaccgatcag
    17881 ccagttcaga cgatgtgggc tttttgagtg tcaggcgtct ggggaacagc gtttcaccgt
    17941 ccaccattga ggccagcaga aaccctttgt tccacagcct ctccacttgc gcacgcagat
    18001 cggccggcgt cgtccagttc atgcttgggc tttcgccttt tctgcctgat attcctcgat
    18061 ggagaggttc cgaagctttg aggaacgacc gtcctcgcta tgcacaaagc ccacgcttgc
    18121 cacgaagggc tcaatgatat gaatcttctg aagaggggtg acgataagga gctgaaggtt
    18181 catctgttga aagagcttca aaccgtactg cgcggactcg tctgagccac gggcaaaggc
    18241 ttcgtctatt acaacaaagc ggaaggaccg cgagcgcaca gcgccccatt caagcccgaa
    18301 ttgatacgcc aggctcgccg caagtatcgt atatgccagc ttttcctttt gtcctcccga
    18361 tttgccgccg gagtcagagt aatgctcgtg ctcttttccg tcttcccgcc accgctcgga
    18421 ggcggcaaaa ctaaaccagt tacgcacatc tgtgactttt gctgtccagc gccggtctgc
    18481 ctcagattgc ccttcacgtc ctcggaagcg ttcgatgatc ctcttgacct gtaagaactt
    18541 ggcttccgaa tattgttcat cctctgaacc ggtcagactg ccttcggtac atgccctgag
    18601 ctcgccctgg aagtcccgga tatcggcgtc ttgcgtggtt tgcgcctcca gtacgatgta
    18661 acggccggga ttatagtcta tctgggtcag tgattcattg atatgagtga tccgctcttt
    18721 aatggtttcg cgctcacggt taagctgcga ctggaagttt gccacctccc ggatggtatt
    18781 ctcattgaga agctctttga aacgcgcctc aaaacgaggc agatcatcag cgtttaactt
    18841 ctccagcatc aatcgatatt cccctaccgc ctcgacgctg acatccacct cctgtgtttc
    18901 gagcggaaag gacgcacagt atgaccgcat ggcatctatg atcttgtcgc gcagacgcgt
    18961 cagatttttc gcctcattgt cgatgcggct ctgcaaccag gtccgcatct cctgttccct
    19021 gttgtcacat gattccacac ttaagtagtg ttcgccaagt gcctcaggcc tcatggcctc
    19081 caaagattcg aatctagccg aattatgtgc gtttaaaggg tcgtcgagca gcgcttgcgc
    19141 ctgagcatac aatgtctcgg cgtcagtctt tttctgttct gtcctggacc tcttgtccct
    19201 gccctctatg agcaaccgct cggtctcctg gatttcaagc gtaagcgcat ccaacctgcc
    19261 ggtcagctcc ttgagcagat cggaggcggt ttctagttgt ctcttttgtt gctggagccc
    19321 tgaaacctct gcagcaaggg gttgccagtc aagttcctga aagtcctgat attcgttaag
    19381 tttattaagg gtctctatcc tggacctaag ctctctctgc tctttttgca acgagcttat
    19441 gtgactgccc atccctgtta atcgggtttc gagctgctgc ttttttgtct ccagtgcagc
    19501 tatcttggcc ttgttggtcc atccaagaac ataacggctt cggtcatcga gccgatggcg
    19561 gtcatccttt tcgtgcctct caccctggac cttgatctgc cctgcaagcg taacagcgcg
    19621 ggtctcgcgg cgaaactgat cttgtgtttc acagcaggca acatcaaatc gatgtgcgat
    19681 ctccctttcc agccagtcat aatattgaga atcaggcttt atcgcgagct tgtgcaccag
    19741 ggaatgctgg tgcagtgcag agacatcgct ccgaccggcc ttgcgcacgc ggaaatagac
    19801 cagcctgccc tttagatggg tgcggtccac ccaggcggcc acctgcgcat aatgcttgtc
    19861 aggcaccaga agtgaaaggc cgaagttacg aagcagccgt tccgcagcgc cctcccactt
    19921 ttgctcttcc tcgcgcacct gaatcagctc gcctgcaaac ggcatgtccg actcggtcag
    19981 cttcagagcc gaagcgatgg ctgtacgcat tgctatttgt tctaccggaa tgttgcttcg
    20041 cctggactta aggctattga tctcggccga aagcgcgtcg tgttcctgac gccattgtgc
    20101 aaatgttacc cctgcctcgt tcaattcgtt ttgaagcgcc gcctcccggc ctgaggcctt
    20161 atcagccagc tcgccgaagg tctttcgttg atccaggaaa gcttcggcgc tgtcggctgc
    20221 tgcgatgttc aaagaacccg taagctcggc gtaacgcgcc gccttgcttc ggcgtttgac
    20281 gcactcatct tccttttgcc tgatttctgc tgcaagccgc tctatccgat ctccaccgtt
    20341 ttcggctatg cttcgtctaa gctcccgttc ctcggccaat tgcctgttct tgcgctcttc
    20401 catgcgctgt acacttgcat tctgccgcga ccattcttca gcaagggatg cgatgcgctt
    20461 gtcgagtagt ccgagtttaa gtttagcgaa gtagctcttg agagcttcac ggcaggcccg
    20521 tagttcctca ctgcgcacgg taacctccgc atgcctgtcg cagtctgcta acagtggtgt
    20581 gagcaatgca acctgctgtt tagccttcaa aaccgcctca tgcgcacggt taaggtcatc
    20641 gaaatgcgta attagcgctt ttatccgggg atctacgtca aagggctcca gcatatggct
    20701 gcgcacaaaa tcggtaagat tgccgacaga tttcatagac accgtctgat gaaagagttc
    20761 cagcgcctgt tcgttgtcaa tgccgaacct gcggcgaaac catgcgccgt agggcggaaa
    20821 gctgtcaaac acttcagcgc ctgcaccacg caagcgcttt ctcagcgccc cgatctccga
    20881 accgaactga gagaagtccg tctcaatcga taaggcacgt gtcgccccaa caaaaaaacg
    20941 cgcgggctgt ccttgcgggt ctttcatcca aaacacctgc gccaacgtta gcgtctggtt
    21001 aaagccggta ttatagaaaa cgcccagcac cacagaataa ctattgtgat tacgcaatgc
    21061 aaccggcctg gccgtgccgc ttagctcact gcgctccgac ttataatgcc ccaatacata
    21121 ggaacgaaga gtgcgttccc tggtttcgga cccggccgct ttgttgtaag cgatgcgctg
    21181 cgccggcacc aacaaggttg ttaccgcatc cacaagtgtc gttttccccg aaccaatgtc
    21241 cccggtaagc aaggcgtttc tgccgttaag gtgcaatgtc catattcggc catcgaacgt
    21301 gccccagttg aaaacctcca gacgatgcag tcgaaagccc gatagtgaat catctgccat
    21361 gaattccaag tccggagact cattcatcct gccggcttcc cttcctgcct tcctgtaaaa
    21421 gtgtccggta atccgccagc cgttcatcga attctgccag ccactgcgca tcaacaaacg
    21481 ccttgagaat gcgcctcact tcaaacattt ccttctgtcc gctttggggc ttcagtcggc
    21541 gcataaagcc aagatccact atcttgttta tgtggccgtc tatctgatcc atcagcttgg
    21601 cctcgttgct gctctcaggc aggaacactc ttataagttc ggcaatgtct tcgccggaga
    21661 ggacgaggcg cgtatcgccg cctgaggcat cgaactcagc caatttcttg cgcaacaggg
    21721 aaagcaaaag gctcaccgga aaagaaaggg accttcttgc gaccagacgc ggcagttttg
    21781 tttccgtttc atctccgttg ccggaatgtg agcgtaaaaa tgcatagcct tcagcctcat
    21841 ccagcaccag atccagtcct atgacagaca catagtcacg aacacgggcc tgtaagccga
    21901 gcaaagagtt ccacaggtcg ggactgcttt cctgataaag aacccctttt agaagcggca
    21961 caaccactga agacaaatcg tagtcttttg aaggctgcat accaaagtct tgctccgcca
    22021 tctttacctc acaaatataa cacgcggcag gcgggcctgt cttatgcgtc cgccatcctg
    22081 gtcagagggt ttccacagga tgacatcggt gatctcttcg tcaactatca ctttgaaagc
    22141 atctcctgcc aactgaagat aggcgatcaa ttccgcaagc ccatgttgca agggtcgggc
    22201 ctctacgagc tcccgcagag taactcttaa ccgctcttgc agtgcattgc ggatatgcct
    22261 taaaaggtca gccttatcga taaccacctg cgaaaagagc gcggctgcat ccaaatcctc
    22321 atcgcccggt tgaagggcca cgtccgtgat caaaggtttg accggaggag tgtagagcgg
    22381 ccgctccatg ggcaattcga tatcggcccc tgcgtcgtct atctgcatga attctccggc
    22441 aggcggtgag tctctaaggt caagcgcttt tgcctcgatg ctgtgtaata tgtccatgat
    22501 gcggcgattt tcaagccatg cctggtcatc aagaaagcgg cgcagctgtt gggaaagcag
    22561 agccactgtg cgctgtgtat gctcgcccgc ctcaagccag tcatagtgta tgcgccggat
    22621 gcgggcgtcc ggcttgagtt cggaaactgc cggtaaagca agcacccgct caagaagctg
    22681 cgtaagttcc tcctgacggc ttccggacat aaggaagtcc cagaaggcgc ggaagctcct
    22741 accctggtct gaatcggcaa tcgcatcgcg ctcgcccata atctcctcaa gcagcactcc
    22801 ctttccgccc tcccaaaggg cgatgcgctc gcgcacatga cggtcaaggc ctcgaaagtt
    22861 atgctccact tccctgaaat ctgaaagcag ttcacgggcc atggcgataa actgctggaa
    22921 gcggtccttc aaggcagtat cggagagcat ctgaatgtca ccggcctgaa tacgggctat
    22981 ctcggcatct atttcttcgt gacgcttgcg taaatctgaa atgcgcgcct gcggatcggt
    23041 ctcgctgccc tcgctcagct gcttaagaag cgtaaacagc gttaggagcc gcgattccgt
    23101 gccgacaaag gccctttcag tcagcgtgcc gatccaggtg atcgccttct ctgctgcgga
    23161 agtcagatcg aaatgaggtt catccgagcc atgtgggtaa aacttgcgga gccagccctt
    23221 ttcgttggtc gcccagtcgt taagatagtc aatcgccggc ttggggaaga catccgaacc
    23281 aaggctctcg cgcaggccga acaactcgtc ctccagtgct tcagaaagac tggcctgtgt
    23341 catgacgcgc acgtttggag ctataaaaac ccggtgcaga aaactcgcga tgagcggcgc
    23401 atgatcagag cgcagcatcc gccatgccgg gtgtttctgg cgcagcatgt caagggtgga
    23461 atagtccata ctcattcagg aacgctcata tttcgcaata caagttacca tgcttagcag
    23521 acggcaacgt cgtgcagtcc attctctatg cttaactttt tgttttagcc ctgcaagagc
    23581 tattataacc catattctgt aagtgggctg tatatgtgca aaacttgact tttcactttt
    23641 attttcaagg aattttgcca ttttattctt tgcaatattt tccgtagttc accgtatttt
    23701 aggatatttt cgattacagt caacgaaagg aaagaaaagc caactcctac aaccaattga
    23761 tattattata taatttggcg gtcccaacgg gattcgaacc cgtgttttag ccttgagagg
    23821 gccacgtcct aggcctctag acgatgggac ctataaagac agtgaatagt caaaggtgat
    23881 cagtaaacag cataaacaga aaaaacgact attcctgact attcccttgc caactactat
    23941 tcactaatta ctatccactt tcaatgttgc taagcaaaat tgaaagtggc tggggagaga
    24001 ggattcgaac ccctataagc agatccagag tctgccgtcc tgccattgga cgactcccca
    24061 aaaacgattc atcaactggt ggtgagaccc aaccctagca ttcttaacga ccttagttta
    24121 taccataaaa aataatactc tgtaaacact gggtggtcct gggaatgagg ttttcttcta
    24181 ggtgctcact gcaattacaa tgcgacgagg gccttccccg cttcataggc atccctgagt
    24241 gccgtaggat gctcaaggat tgcccccttc gcatccaccc caagaaatga cagtgttctg
    24301 tgttcgggaa cactcactgt cctgaaaaac gcctttgcag tcgcctcccc gcactgtatc
    24361 cctatttctt tcttcatgcc gcccaccagg aggaggagcc cttttctgcc aaaggggcct
    24421 tcgggtatcg tcttcctgag aagatatttc tcacaccaga aggactgaca gcggtctatc
    24481 attgcctttg actgcgcgct cagcccaaag aagaagatgg gagatgcaag gattatcctg
    24541 tcggcctccc tgatggcatc gtagatctca tccatatcat cgttaataat gcatgttccg
    24601 ttcttttcac accccccgca atcctggcat ggcttgatat tcataaaatt gagcttaaag
    24661 acttttatgt catgcccctc ttcccttatg ccccttatcg cctcattgag aagaatctct
    24721 gtatttccct ctatcctcgg gcttccatga aacacagcca ccttcattaa cattccactc
    24781 cctttgttct gccttttaac ccaataccga ttttcaaagt tgcccgcgta tgcttttatg
    24841 agactgaatg cgtgactaca atgaggaacg cttcttgcca ctctcgggaa aagaagctca
    24901 gggatgtatc atccctcaga acttttgatg gtattctttt accttctttg caaactcttt
    24961 cccgaaatca tagcactttg tttcgtcctc caatgagggc ctgtaaagga cctgcagccc
    25021 gggatcaaag gtctcaagcc ccatcctctt aaactcttcg tatgcctcct tcactgcacc
    25081 gccgccccat ccgtaactcc caaatgcccc cataatacgg ttcttcggcc tgagacccct
    25141 cagatgggtc aggaattccg ccacagtggg atacatgata ttattgagcg tcggtgttcc
    25201 cacaagacac cctctggatt tccaaaattc cttaatggcg atactcatgg gcgtagctct
    25261 cagctttatc accttgcact ctaccccctc gtcctttatc ccctgcatga tcggcaatgc
    25321 catcttctct gtactgtgcc acattgtgtc ataaattatg gggacagaga gattcgcctt
    25381 gccattcgcc atgtcgaggt atgcctgtag taccttaccg ggattggtcc tccagatgat
    25441 cccgtggtca ggggcgatca tctcgatctc cagccccagt ttctggattt ccgcaatctt
    25501 tgttttcaga acctgaccaa agggcatgag gatattggca tagtaatcca caatcgcgtc
    25561 gtccagttct gacatggatt cacaggtcac aaactcgtca tcaaaccgta cggaggaggc
    25621 gatgtgctgg ccgaatccat cctgactgat gaggatcttt tcttctttta tatacgtcat
    25681 catagaatca ggccaatgga gcattggcgt ctcgatgaag aggagcgttc gcttcccgat
    25741 cgatagcgta tcgcctgtct tcaccacctt caccttccat cgggaaaggt cataaaaacg
    25801 ttcaagaccc tttctccctt tctccgtgat atagatggtt gcattggggg ccagctccat
    25861 tatcctgtca atactcgttg catgatcgtt ttcgatatgg ttgatcacca cgttccgtat
    25921 cttggatggg tcaataacac tcgttatgtt ttttatcgta atgtcccaga agtcgtattt
    25981 gacggtgtcc agcagggtta tttcgtcatc aatgatgaga taattgttgt aggttgtacc
    26041 gcgaggggtc gcatatccat ggaaatccct caccgcccag tctatgacac caacccagta
    26101 gacaccattc ttaagctcaa ccgccttcat gctttcccct ttctgcagta aaaaaatagc
    26161 tcgctacgcc aacgtcttga acgaccgtcg ggaggcgttt gctatgggtc tctctcagtc
    26221 ttccaacaat gtcgagaata tattgtggtg ttcctcttca tccgcaagta tggcttcaaa
    26281 gagctttctg gtcaccacat caccttcttt acccgcgaga gcaattattc ccttataaag
    26341 ctttattgca ccttcttctg ccttcttatt tatcttgagc atctcttttg ccgtcttccc
    26401 tatcgcaatg ggtgtcggtt ttgtggttgg cactccccca agataatcga gccgctctgc
    26461 gatggcctcc gcatgcttca tttccgtgat cgcggtcgtc ttgaatacgc cgcccaccga
    26521 ctcggcattg atacccctga tcatcacatg ctgccacatg tactggacac ttacctgtat
    26581 ttcccttgcg attgcgtcgt tgagcttgtc cttcagttct ttgctcgcca taagtcttcc
    26641 tccacactct tcattgcggg ccagaaacca gccttctaaa aaaatgcatc gtaaaaaaga
    26701 cgattcttac ctatattact atatttgccg attaacttct atccagatca atgacagaga
    26761 ggttcagtct tattacacca gtccacggtt catttcttta atgtctcctg agcccctttc
    26821 tttccctctt cccaatttca gggatattcc cgaaggcatt gtttcacctc ccacaaaaag
    26881 ttttgtcctg taaatctcta cccaaatctc ctcaccgcat cgaggacctt atgcctcacc
    26941 ctttcagcaa agagtacctc tgcgtggttg acattaaggg tatggtgttc atcagccgac
    27001 gggatccggg aactctccac agacacaaga ccatcaccct tgcccttttt catctcatca
    27061 gggaacagcc tttccgggat gaatctttca aaggcatcag gaactgaaaa aactgtttgt
    27121 ggcctcacca cccatcgttc ccgatccctg acatgtgtcc gttccattat ccttttatac
    27181 acagagaaaa gcgtcggatt gctccctcca aatgagagat aatatacccc ttgcaaagga
    27241 ccttcatcac aagatctaaa gaggggcgag tccggcaata gttcccttac tgctttgctt
    27301 ttgaggaaat caaataccct cttcaccgca taggtgaacg ttcctttttc ggaatcggat
    27361 atcagggaat cgataacggt tatcaggggc gcaacatgtt tcacccaaag ggccatgttg
    27421 ctccccctgt ggggggtggc aagggtaaca atgcctctta tccctttcgc atcacctttt
    27481 aaatagtttc tggccaccaa ccctccccgg ctgtggccta taaggattat tccagatctg
    27541 cagtgttcat gatgcatgct caccacttcc ctaagctctg acaccgcaac ggcgatctcc
    27601 gcagcaggcc tctgctggct ccaggctatg actgtatacc cctgttcccg gaaatcatgg
    27661 aatagagtgg cgagatgttt tggcggcttg ccaaaaaaaa gtcgctttgt aattcctctg
    27721 tgagtgtccg ttttcagtat ctccggtgct ggctttgtgc tcacaaggaa atgaatggga
    27781 aactttccgc ccagaatcct cgacgtatcc ggtgactccc aaattctctt atccattccc
    27841 aggccgtgga taaaaatcac tgcgggctta tccgcatctc ccctgatcag ggtggtgtcc
    27901 agcttcatag gcttagttat aacaaaagct aatgaattta taaaggattt tttatttaca
    27961 atttccttcc ccaagtgata taaaatgaaa ctcccaaaaa ttttcccagg aggtttttct
    28021 atgaaattag gtgtgcttgt gagcgacttc aggaatatgg acgacacatt atcacgatta
    28081 aaggcggata agctcggtgt tatcctcgtt agcaatggag tatatcatgc agctgcaaag
    28141 gagggtggca agaaatcttg ggttctcgaa aaatctcccc atctctatgt gctctctgaa
    28201 gacctgcagt caaggggatt ttctgaggca gagattgaca ccagagtgaa atccataaca
    28261 tacagcgatc tcgttgactt gatattcaac gaatacgaaa aaattatttg gatataggag
    28321 gtatcatggc caaattaacc atcggagttt tttcatcact tgtcggttct ctctccctcg
    28381 atttcgcagt aaagctctcg gaagctgcgg tcaagaaggg gcactcggta gatttctggt
    28441 tatcagggaa cgcaactatg ctttccttaa aaggccagcg gacttttaag gattattctg
    28501 cactccagaa gcccgttcag gagcttttag cgaccggaaa gtttcagata accgcatgtg
    28561 aggcgtgcgc cgaggcgaga gggtaccaca aggaggatac catggagggg tgcaagcgcc
    28621 aaagcatgga ctggtacctc gcaagctcct tcagtgcaga cagggttgtt catataggag
    28681 gtgattaata tggcaacaaa aaaacttgca ttcattgtga aaagtctccc ttataaaacc
    28741 gaggcgtcga ggctcgcatt aacacatgcc attgcgagcc agactgtgga gatttacctg
    28801 gaggacggtg acatggtgga gcctgttctc gcattcatgg gtgacggcgt gttaaactgt
    28861 cttaagaatc aaaaggcaat gactctttat ggagtcacga gtctcgaaca acatataaaa
    28921 aattcattgc tccttgactt gaaggtgatg atctgtaaag aagatgtgga gaggtttggc
    28981 ctgtccgaag atgcattgat catggatgcc gaagatatcg ggggagagac caacgccaac
    29041 atcgcgccat tcaacgagat tatgaaggag atggactctt cagaccatct cctattcttt
    29101 taaggaggaa ctatgggaga actgagcagt gcaacaccgg cacaaacact tgatgtcctt
    29161 ggaagggtat gtccgtaccc cctggttttg acaaagaagg ccatggagaa attaccgagc
    29221 ggagccgtca ttaaggtgtt atgtgatgca cctgcgtcgg ctgaggactc tattccaagg
    29281 tatgcggaga aacaggggtt caagttcgag tcggtgaaga atgagggcta ctgggagctg
    29341 tacatacaga aaaagtaaaa agtccccgga gatttcctcc cggcaacttt catctttctg
    29401 caaatgacga agggggatgc cgtttaaggc atccctcttc gtgttgtttc tgcgatcgtt
    29461 actgatccta catctctttg atcgttattg cattggcagg acagacgccg agacagctct
    29521 cgcaaaacac gcactcagcg gtctgataag gatctgattt gccatcgctc atctgaaata
    29581 caccctgagg gcagacattc acgcactcct cgcagccttc gcatgttgct gcgtttaccg
    29641 tcaccatata cacttcaaaa tcacctcctg atcgttatac ccgctcaaaa cggacttgat
    29701 agtctaaatg aaatctaaac gccaaatcaa tgaaattctg ggaggaagaa ctcttacttc
    29761 ttctgcctga gaggattgat cgcgagcacc ggggcaggag agtttcttgt caccttttca
    29821 ctcacactcc caaaggggaa atgtttcatt tccccacccc tctcctcgcc atgaccatgg
    29881 gtggccatga ccaccaaatc aactttctct tcattgataa acttcaggat ctctttggca
    29941 gggtcgccta caacgatatg ctttttatag agcgggcaac tcgcgagact ttcctggcag
    30001 atcttctcca tctgctgccc acccttctga attattttct cctgaaattc cttaagaaag
    30061 gtggggctgg catcaccata gaaatggtcg tactgatgca catcttccac cacaaaaatg
    30121 agatgcacct cagcctggta cttttcgctc aatgaactca cataaggcaa tgcctgagct
    30181 gcgttcctgg aaaagtctgt tggccagaga atctttctta tttccatgat agtcccttct
    30241 ttgtgcgatt agtgttctat ttctatcccc tcggtctctt cccttaccga gatcgcaatc
    30301 ttgtaaagca aagtaaggac aagaaaccct aaggcccata ccccaagggc aatggatgtc
    30361 tcacggagtg tgggccaata ttccgtcacc ccttcgaagg ggttcgggat aaatccgccc
    30421 accacgagac caaatccctt gtctatccag agggaaataa acaatgcacc gcaggcaaat
    30481 gccaaaatcc gttcggaccg acgccattgg ggaacgacca ggagaagaag ggataccacc
    30541 gcaaggatgg tcgaaatcca catcaggggt acaagctttc catgtccctc aaggccggtg
    30601 taaaggtatt tgaatggatg cgtatgccca ggaatttggc tataaaatgc ggtaaagaat
    30661 tccagtccaa ggaaaaatac attcgcgatc atcgcatagg tgacaatctt acctactgcc
    30721 tggatgggct ctttgcctgg atcgaatttg gtaaattttc tcacgatgag cgccagcata
    30781 atgaggagtg ccgggccacc cgcaaatgct gaagcgagga accgtgccgc cattattgcg
    30841 gagagccaaa aatgtcttcc agggagaccc gcatagagga acgccgtaac cgtatgaata
    30901 ctgaacgccc aggggatgga gagataaatc agagacttta tccatgaagg gggcttcact
    30961 cctttgcggt ctgaactcaa ggtgacccat ccgatcacga tattgaggaa aagatatcca
    31021 ctcagcacaa ccatatccca gaacaggata gagtttgggg ttgggtggag tatcacattt
    31081 aataccctca taggctggcc aagatccaca aagataaatg aaatacacat cgtaactgaa
    31141 gcgaccgcca ggaactctcc caggataaca atcttcccaa atttcttaaa gtcatggaga
    31201 tagtagggta tgacgagcat caccgcggaa gcagcgacac ctacaaggaa ggtgaactgt
    31261 cctatataga ggccccagga gacgtctctg ctcatccctg ttattcccag tccattcttg
    31321 aactgataga ggtagttaat gaaaccgatt cccacaattg cggacaaaaa gaatagccat
    31381 gtccagtatc ttcgacttcc tacaagtgcc ttctcaagca tttttcgcac ctcctatgat
    31441 gtagaaaaca ctgggctgag tcccgagttc aggcttgcgc ctgatggaat acctggtact
    31501 gagaaccttc cttacctcgg aagaggggtc ttcaaggtcg ccaaagaaca tccctggctc
    31561 cttccccgct tcctttgctg ccttttggga ttcctccaca catgccggga taagcccttt
    31621 ggaaagtctc tcgaagcaga aggtgcactt ttccacaaca cctattgtcc ttgtgggata
    31681 ttccttgttc tcctccttga gagacggcct gggatctttg taattgaaac tcctcgcccc
    31741 aaagggacag gccgccatac agaaccggca tccgatacag cgatgctgat ccatcatcac
    31801 aataccgtcc ttccttttga acgtcgcctt ggtggggcag accctcacgc aaggagggtt
    31861 ttcgcagtga ttgcagagaa ggataaaagg tttatgtttt acctcttcag gtacatattc
    31921 attttcctgg cccgggaagg tgtggtcata ggtgtcggtc catatccatt tgatcgagtc
    31981 cttgggatta tcaagattgg gaacattatg gatgctgtga caggcgtgaa tgactctcct
    32041 gtaatcttcc tctgtttcaa acttgctcat gtcgatcacc atggcccagt gtttcccggt
    32101 cagggccttc tcgtcagtca acacatggga ggcctcaaga cggtcattcc tgagccagtt
    32161 gataaccggc gcgcctccca ctccaaggat ggtcgaaatt cccgctatct taaggaattg
    32221 tcttctgtca atactcatgt ctctttctcc tttggcgcga tatggcagtt ccagcaatag
    32281 ggattcaccg ccatataggt gtggcattca tcgcagaact ttttcttgtt tgaatggcag
    32341 tgcatgcagg tattctggag actaatggtg tatgccttcc cttcggtatt cacatatagc
    32401 cttgcgccat cccgtaccgc agagtctctc cagtcattca gcaacttcat gtgctcggcc
    32461 ctcatgaact ccttggactc cacgcacttc ttttccttca gttccttgat ggcaggggta
    32521 tcgatcttcg gatcaggtct cacatttgct ttcccgatat tatagaaaaa ggggaatgtc
    32581 acaagaccga gaaagatgat aagtccggtg attattttgc cgccgttata cagtttcatc
    32641 ggccgttccc tccttcccgg gaagaggctc aagtcggata tcaacagtcc tctccttctc
    32701 acccttcatc acaagggcat tgcccaccag ttcgtgaaca cccgtcactc ctactccggg
    32761 gacccagtaa tcaatggagg tggtgagtgt agccctgtca atggcacaca cattggcaac
    32821 catattcacc ccataatgct cgtggacata tttcaccgca tttgccctcg ggaacccccc
    32881 tcttaacctc agttccatgt tttccgaagc attcaagccg gtaccgcttc cgcagcagaa
    32941 ggtcttttcc cttatggtat cttccggcat ttcatagaaa tggttgcaga cattcttgac
    33001 aatatatcga ggctcttcca gcagtcccat ccccctggct gtgttacagg agtcatggaa
    33061 cgtcacctta aggtggtcat tccggttggg atccagcttc agttttccgt tcttgataag
    33121 gtcagcagta aattccgcga tgtgaaccat cttcgcggcc tttgcgtttt cgaatttcgt
    33181 gccggttatg ggagagaccg gttcttccag aaaatctgca ggcccgttcc agctgtccat
    33241 gtactgatgg ataactctcc acatatggcc gcattctcca ccgagaatcc attttactcc
    33301 cagcctcttg gcctcggcat atattttgta gttcagtctc tttgccatct cattcgtggt
    33361 aaagaagccg aagttccccc cttctgaggc ataggtgctc caggtatagt caagtccaag
    33421 ctcatggaag agcatgagat agcccatagc agtgtaggtg ccgggatttg cgaaaaggtc
    33481 tcctgaagga gtcacaaaga gaatctccgc ccctttcctg ttaaaggtgg ggtttatctt
    33541 gatccctgta atggtctcga tatcgtcaag gaagaattca atgctggaag taatggtatg
    33601 gggctcgaga ccgaggtggt ttcccttcat gtaacaggct gctatgggtc ctgcgatcca
    33661 cccgatattc aaccccagga ggttggtgag ctccctgccc atcatcgtga tctccgcctg
    33721 gtcaatgccg tacggacaga acactgagca gcggcggcac tcagtacact gataaaaata
    33781 gtaccaccat tccttcagca cgtttaccgt gaggtcccgt gcccctgcaa tcttacgcag
    33841 gatcttgccg gcagcagtaa aattcctcct gtatacagac ctgaggagct cagccctgag
    33901 cacaggcata ttcttggggt ctccgctccc gataaagaaa tggcacttgt cagcacacgc
    33961 accacatctc acacagatgt ccataaagac cttaaacgaa cggtacttat ccaggaggtc
    34021 tttcattttc tccaggagca gcctctccca gttttccggg agcttccagt cctcatcagc
    34081 aggcctccat tcccttgcat tggggaagcc cactatttcc agctccttgg gttttgcagc
    34141 gtggcagaac atgtgctccc tgatctccac cggggtgtcc atccacccgg ttttcggagg
    34201 ggaataatct aaatttgata gttcattggg ttttgcggta aactttgcca taattactcc
    34261 ttttccactg ggatcccagc ccctttcatc ttttccctga actcttcctc atattcctca
    34321 tagctatgaa ctttcacagg ataattccag gggttgatat gtcttttcat gcgactgtcg
    34381 ttcgccatgt tcctcgtggg gctgaggaag atcccgccca tatgcatcag cttgctgaaa
    34441 ggaaagtagg ccagaagtgc gctgacgagg aagagatgaa tgtagaaaat aaccccggtc
    34501 ccttcaagca ccttggggtc ataactaaaa ctgattaatc ctatagccag ttccttgatg
    34561 cccgtcacat tcaccttgaa aaaatacctc ataagaaccc ctgaaagcgc aatactgaaa
    34621 ataagaaaga gggggaagta gtctgcgggg agagagatat agcgcacctg cgggagcaat
    34681 actcttctta taaagagata tgtcactgca gcaaggatca ctgcatcagt aatatataaa
    34741 agaggagcac cgatctggag gaaactatcc agcccctcga tcatttttat agggacaggc
    34801 accttctcga aaaagaatct catatgtctg accacgatga aaagaaagga ccagtggaac
    34861 gccagtcctg ccagccaaag ccacttcgcc tctccatata caagctttgt cccgtcatag
    34921 agctgagttt tgaggttcct gaagagggaa cggaaaagaa ggacctccag ggccatcctt
    34981 ccgataacgt caagggtact ggaggggctt tccagcttat tttgcttgat ccatggcaat
    35041 gatttctcct gaccacaggt ggtgggtatg cagaatggca caggcgaact cccccaccaa
    35101 aagaggacgc ggtaaataat gcccacaagg aacgtgccaa aagctacata ggggatgaga
    35161 actccaaaga ggaagtgcag gctggcccct ttcacgccaa tgaaggcgat aagtgtaagt
    35221 ataatcactg caaaaaacgc aaatacggat cccatggtat tacctcttgt tatcctcggt
    35281 taacaacatt atcacctact cagtccgatt gttccttttg cccctcagat accagattcg
    35341 ccatctgaag cagcctgaag gtcctgtttt ttacttcgtt cgacttgagt tcatacacct
    35401 tttccctgca tttcacaaag atatcaaagg agatgagggc aatggcatct atcctggact
    35461 caagtgtcgc catctccgcg gagaattcct ttgcctctga tgccagctct tccctgatcg
    35521 cattcctcag gtgaaacaca aaggagagag cctgcgacgg tctcagatcc tgcacagccc
    35581 tgatccgcac aataccttcg agaaagggaa ccatcccctc aaagtctggc cctttttcat
    35641 caatgagctt gtcaagaatg ttttccaatc catgggaaat tgtgtatcct accggattag
    35701 tgaactgttt tttttcattt ttcaaaaggc ctgcggtatc cgaaggatat ggctccatgg
    35761 cgatatcaaa ccatctcttc acaagagcag attttttctg ggccaggaaa tcccagagag
    35821 tcatggcacc acccatgcgg gaattagtag cggtactttg aggagggtcc tcagagaaac
    35881 agcatacatc gcatcacaca tatggttcta ctctttcccc tcccctttca tttgagcaat
    35941 acaaacttga ggtgagatag gaggccctat ctcaccattc gcagcgcgta tctgtctata
    36001 cacatcctgt cggtttcgga agtccggcaa tcttacatgc ctgttttgca ggcccgcccg
    36061 gatacagctc gtagagatac ttggtattcc ctttttccgg acccataacc tttccaagtt
    36121 cctttaccag aatctttatc atgggtgcaa tctggtactt ctggtaataa tccctaagga
    36181 aattgaccac ctcccagtgg gcgtcggtca tctcaatgtc ttctgttttt gcaatgtgag
    36241 ctgccagttc cttgctccag tcatcgaggt ttataaggta accatcctcg tcaatctcat
    36301 actgtttgcc ctgaaattcc atcattggca taacaaattc ctcctttcta ccttttatct
    36361 catttgattc ttctcactcg aagggagccc ctccttgtat atccgcgatt gataattgct
    36421 gagaaagctc actcacaaga agtgttacac aataatacaa tggaccaccc atggtcaaat
    36481 aaaaataact tattggcaaa tttgtgtcat ttatcatttt ggcccgacct ttatgaggat
    36541 gatcttgcct gtataatccc ttcgcatatc ccttatttcc cacatcctat gcgttctcat
    36601 atcatagtta cgcaccgctt catgaaagct ctcggcatca agggattgca ccttattcca
    36661 atccccgccg gatgcgtaga tcagcgcgtc aaggtatgcc tcgcccacat tatgcccggt
    36721 cgggtcacat gcatagacag ccccgcactc acatttccct ccgatgaatt cccccgtatc
    36781 agtcctgatc gtttctggca taggcagtgt tccgctacaa aagggacatc gagccgattc
    36841 atccttgtct tgtcttagcc ttggcttcaa tcaaccctcc ttattgagat ggatgattta
    36901 tgtgtaactt tgctcgtcca ataccctttg tgcaacatcg cctactgtcc tttcaaccaa
    36961 ttcgcgatcc tcatacaccc tgaagcgctc cccgtcacga atcaggaagt tcaccttgtc
    37021 gctcattatg ttgaacgttg aaacagcaga gagtgcaagc cccctcacca acgggcttct
    37081 gtgtgagagg gactgtatca cgagttcctc agcaccctct ccattgttca tcccggcccc
    37141 tgcaatcctt cctatcgccc agaggactcc cggaagaaag ctttcttcat ccttaaatga
    37201 gaggagtatg ggcgggatat ctctacaagt ttcgggagta ttgatgacaa tctctcctag
    37261 catctcggca gcactccatc caatgcctcc ggactcctcg cccatggacc atagaagacg
    37321 ctggacgata tttctcactg ttgacggatc cctctctgca acagcgcccg cagcaatccc
    37381 catggcttcg atagctctcc agcataaaac gctttcctta tcatacgtga atgagatcaa
    37441 tatactgaag acttttttgt tttccaaggc acgctgcaca acctttgcaa aatcctcttc
    37501 aaagagtgca tctcttatct cttccttcaa cagccccact cgtagactct cgataccctg
    37561 tgatacataa cgcataaatt gtcactccct gtgcgccggt aatacatttt ttcttatgag
    37621 tatctcctca cggttttttt ctcatcaaac ccaccggtct taatggcccc tcaacaaacc
    37681 cttcaggcac tgagcgaagt ccgggttttg ctgaatttct tcgccagcct taccatcgcc
    37741 tcaacccaat ccggtgcacc atacgcatgc aggtgggtgt aagtggcaaa cacattctta
    37801 tacaatagac catccacgcc accgccgatt ccattccctc tcctcatcct gaacgctgta
    37861 gggagcatat catccccagg ggggttcaga acagcagaat aatggaattc atgtccatgc
    37921 atgatggttc ccaccggata aaaaggattc tgttgttcca cctctacaac ggtatatcca
    37981 tgggcctgcg gcctggtcct catctcgaac ataaggggga agacgcctgc catgggatac
    38041 ttctctccat tcaccacaag ggcgtcacca aggaacataa gccctccaca ttcagcatac
    38101 acgggaagcc cttcttcgat agcgctgcga agggcatttt tgaattttac gtttcgcgca
    38161 agggctaagg catgggtttc cgggaacccg ccccctatgt aaagggcatg gatgtcagga
    38221 agcaggtcat cttccagcga gtttatttcc acaatcagag ctcccttttt ctcaagttcc
    38281 tcaaagtttt ccggataata gaactggaat gcggtgtccc gaatcactcc gatctttacg
    38341 ttcctttcat cagcattcca cttgaaccct gcacgatctt tatctggcag cggttctgct
    38401 tcgttcgcga cctttagaat cccttcaatg tcaagatact ttattgccat gccctccacc
    38461 accgatatcg cgcggtccgt atcatcgtgt tcctggtagg gagtcagccc catatggcgt
    38521 tcggggaggt ctccagagcg cagtctcggc accgccccca gcacaggcaa tgcacagtat
    38581 ttctcaatgg attttctgac taccgcctca tgtctgcctc ctgatatctg gttcaggact
    38641 actcccttaa tgctgacttg cgtatccagc tgcatacagc ccaatactaa cgcggcggct
    38701 gttctcgtca tcttagtaca atctatgacc aggatcacgg gagacttcaa cagctttgag
    38761 acctctgcag tactgtatga gccttcttca tccacgccgt catacagtcc tctgttgcct
    38821 tcgattaccg caacatcgcc gtcaaaatgg gagacaaacg attccaatgc cttttctcta
    38881 ggcatgagaa aggggtcaag gttatagcat ggcttgccag cggcatgggc gagccacccc
    38941 gcatctatat agtcaggacc cttcttgtac ggaataaccc ttaatccctt gtgggacttt
    39001 aacgcagcaa caaggcccag ggcaatagtg gttttccctg atccaccctt gagcccggca
    39061 ataaccagtc tgggacaatt aagcatgatt tattataaca ccaaggtgca taaaggagag
    39121 atgacctata gcggacaagg attttcgtaa gtagtacggg aagcagcgtt gagagaacca
    39181 ttctcctatg gggaaacttc aataaaaaag ggggggagga cagcatcctc cccccccttt
    39241 tttatctgct ttcgctaatc ggttcgatac ggaccgcaca gctcgggacc gcaccgtcgt
    39301 gggcaatacc cgtgaggtta ttcacattcg ctaaagggaa gtggatgggg acataggcca
    39361 cgccgggttt tgcgtcactg gtaatgtgcg cttttatcac tattgaaccg tggcgtgagg
    39421 tgacggtaac aaaccggttg ttctggatac cataacggac agcatccttc tcgctcacct
    39481 caataaaccc ttctggcttg acctttctga gatttctcga agcgagggaa agcgtcccgg
    39541 agtgctgcat gagggttacg gtgatcatga gaatggggta cttttcgtcc ggactctcaa
    39601 gctgacgaaa ctcggtaggt accaacctcg gcaaatcagc ataggcgtcc acctcaattc
    39661 ttgctatctc ttccctgagg gaatgaaagt cattcttgcc cacactgggg tcaaagagac
    39721 gcgcaagctc attgagcgcc tgccatgcag gctgcgcctc gccagggggg gtgagcatct
    39781 tccttgtcat ctgcgaaata tcggccgcgt tcacgtatgt cccgtccgtc tctacccaca
    39841 cggcagtggg caacaccaca tgggcaagct tagcggtctc tgtgagccat tcgtcctgga
    39901 caacgagaaa atcgagggcg cttatcatct gcttcacccg gctcgcattg ggaaaattga
    39961 ccactgggtc ttcccccatt acgaacaggg cgtttatccg gtctggttca tagagcatct
    40021 ggaaggcgtc cttgccacca ggggtaagct tctgaaaccc agggagacga tcgggtgcaa
    40081 cccccatctt ccacatgccg aagctattgt tgtattcaca cggtatttga agtccccggg
    40141 ggccttcgcc ggtgagcatg aggagatttg ccgcggtaag caccgtattt ttccccttct
    40201 cgttttcgtt cgccccaagg ctcatcgcaa taacccgtgg agacgattca agaaagagcg
    40261 aggcagtgtc caccacctct ttttccgata tgccggtcgc agatgccaca tatgatggcg
    40321 taaaagaact tacagaagcg gcaagctcat taaagtgggg gattgaagga gcgtccttct
    40381 tgagatgggt tccttgagca agagcgagat ggataatacc cttgagaagc gcctctgaag
    40441 tggacgggtt aacagaaagc cactggtctg catgtatggc taattttgtt ctccttggct
    40501 ggaccgcgat gagcttcgtc ccctggtgct tcgccctgat gaaatttaat gaccatacag
    40561 ggtgggtagt atccaattca gactctataa ccattactac gcctgcacct attggcgcgg
    40621 tctgctcaac agagagagcg gtaaggccaa agacctcttc cacagcctcc tgcatctttg
    40681 cgaacccaaa acgcgcgata gagtcaatgt tgtggctttc cagcacctgg cgcatgaatt
    40741 tctggagcat gtagttgtct tcgttactga accgcggaga accaatggcg ccgatacgct
    40801 ctgccccctt cttctccttt atccatcgga gagactctgc gatatggtca agggcttctt
    40861 cccacgaggc acgctgaagc attccctcgc gtcgaataag aggtgtggtg ag
//
LOCUS       DATKOU010000220        13162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4529730, whole genome shotgun sequence.
ACCESSION   DATKOU010000220 DATKOU010000000
VERSION     DATKOU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4529730"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..470)
                     /locus_tag="VMU21_10580"
     CDS             complement(255..470)
                     /locus_tag="VMU21_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HUO78006.1"
                     /translation="MSQWPSCKARNIFAALLRIGWYIKRDTAGSHRVLSRSGFPDYVF
                     AFHDNDEIGPRMLARIAKRTGLSPIDL"
     gene            complement(467..667)
                     /locus_tag="VMU21_10585"
     CDS             complement(467..667)
                     /locus_tag="VMU21_10585"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HUO78007.1"
                     /translation="MKIEIEKEDDGRWIAEVSDLPGVLAYGKTRDEAIAKVEALALRV
                     IADRLDHGESIPELDELFALSA"
     gene            complement(1024..1581)
                     /locus_tag="VMU21_10590"
     CDS             complement(1024..1581)
                     /locus_tag="VMU21_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78008.1"
                     /translation="MKKIAVLLWNLILIPPVFAGEIIQQDIPTFTDQDLEKYKKISEP
                     DVTPHDRNKTEMSSSSEDTGIREGAVPASEDKISESEKKSIEQEFNRIVAAMISQLRA
                     GNIEAALAFFVESRKDKHRQIFKALKDNNTLKAALEGYMGVEIRWVSDHLAECELTKK
                     ENGEIFAYPINLMETAKGVWKIYDF"
     gene            complement(1883..2407)
                     /locus_tag="VMU21_10595"
     CDS             complement(1883..2407)
                     /locus_tag="VMU21_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78009.1"
                     /translation="MKTIGLVILISLSLCNVGAAQAPAYEKKCTAVVNGETAQFSFPI
                     PQGQTLTWDMNETSDDVMEYSWELCLERSDIQCKYRFGVLHFKFDRSEKKGNIRELIS
                     SSQKSVWDASNWVQTEMKIDATIEHDNLIIRITDQKTFAEIFASNPKMARCKVKTPYK
                     DLNYESKTGIKYNK"
     gene            complement(2505..3419)
                     /locus_tag="VMU21_10600"
     CDS             complement(2505..3419)
                     /locus_tag="VMU21_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78010.1"
                     /translation="MGKRWKRKMKMLFEVIFIILFSFTSALAVSIDDLNKTVVFLRQQ
                     SQKMIPMDNKPAGLYYRYPDSKEYKPIIETSLGTGFLIKYIGRDYLVTAKHVAKNLLS
                     TGEIIINLPNGNTTIITFQQLSDANIIKRAHWFHHPKADISIHPMGYPGAVDAAALPE
                     DLFPKKDADIKLLTPAYIVGFPLGLGVLDKLSPVAKEAKIASKLTSIANPDIPADLHF
                     YLLDQALSQGYSGAPVFYVEDVMSGVMIGNQQMKGGEILHLLGVQSSALSDQTGGKIS
                     VVVPISYIWDIFESSDFQAYIKGVTSKK"
     gene            complement(3750..5513)
                     /locus_tag="VMU21_10605"
     CDS             complement(3750..5513)
                     /locus_tag="VMU21_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78011.1"
                     /translation="MKRILFRRILVSYLIIAPLLFLFLELYLSNVVKSTHVSELKQSL
                     LVQGNLIAEEIPLTLPVDLDDFCKKYKEKTGARITIIDSSGRVIGDSDESSAKMENHA
                     DRPEIRDAAIGNAGSSIRFSKTLGKNLFYLAIPVIHGSEKIFLRLSMPLHEVEVAVAK
                     TRMSVLFASFAVLFIAILMGLFQTRRITKSIEEITAFSREVTTGHLGRRLFLKEKGEI
                     GELAKNINDMAHEMKARLKQSEEERHKVEAILRNLSDGLMLVDTKGRILLCNEAVRKL
                     FAIETEIEGSTVAESLRNAELMEVIDEVIREEGPVSHEISVAHPRELSLMITSTPFYA
                     YSVNEKLSGVVITLHDITRLKHLEEMRKDFVANVSHEIKTPITAIKGFAETLLDGAID
                     DKENARKFLETIKNHSERLNSLVSDLLTLSRIEMGDIDLHKHNVNINNIIDTVFTTLR
                     DKAEAKGLFLKKALPPEPFEISADRDKLIQVLLNLVDNGIKFSDEGGVTVGMEKDGEH
                     TILFVEDRGTGIPRKHLPRLGERFYRVDKARSRELGGTGLGLAIVKHLVKAHGWNMEI
                     ESTQGRGTKVKILTSENSPSA"
     gene            complement(5510..6211)
                     /locus_tag="VMU21_10610"
     CDS             complement(5510..6211)
                     /locus_tag="VMU21_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO78012.1"
                     /translation="MNSRNVLVVDDEADLVELVSYNLKKEGFAVDSASDGEEALSKIR
                     KKKYDLLILDLMLPGLQGMELCRILRNDPKTSGLPIIMLTAKGEEVDKVLGLEMGADD
                     YMTKPFSPRELVARVKAVLRRSPGERPSGEGMLKIGGLEIDRERYGVSIKGKPVKLSA
                     TEFKLLLYLVERKGKVFSREQLLDAIWSDEAFVEPRTVDVHIRRLRSQIEEDPANPKY
                     IKTLRGIGYFFAEKE"
     gene            complement(6211..6492)
                     /locus_tag="VMU21_10615"
     CDS             complement(6211..6492)
                     /locus_tag="VMU21_10615"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HUO78013.1"
                     /translation="MQLARVHLLIRGRVQGVYYRGFTRDIASHLGIRGWVRNLSDGSV
                     EAVFEGNREDIEQAIKHCRMGPPGAGVEDVVEAWEDYKGDLQGFQIRYY"
     gene            complement(6517..7215)
                     /locus_tag="VMU21_10620"
     CDS             complement(6517..7215)
                     /locus_tag="VMU21_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO78014.1"
                     /translation="MLRLEDVHTFYGNAEALRGVEIGVGQGEIACMIGNNGAGKSTTL
                     MAISGVIKPRGGRILFENRVISNLPPHEIVKLGISQVPEGRRIFPRLTVRENLEMGAF
                     LHSSKTRANYERVYSLFPILKEREKQPGGTLSGGEQQMLAIGRALMSNPKMLLLDEPS
                     LGLAPIIVSRIFKTIKEINDEGMTILLVEQNAHAALNLSGKGYVLESGKISAQGTGVE
                     LLRNEQVKKAYLGE"
     gene            complement(7202..7975)
                     /locus_tag="VMU21_10625"
     CDS             complement(7202..7975)
                     /locus_tag="VMU21_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO78015.1"
                     /translation="MYLLEVRGLTKHFGGVKAIEDVSFRVEGGSIASIIGPNGAGKTT
                     LFNCLTGITKPTKGSLSLSGTDITGLPSHEIVKLGIARTFQNIRLFREMTVLENVLIS
                     QHRKINTGIFGTLLKDRAFLSEERAAREKALEYLDFVGLCEAAHIPSLNLPYGGQKRL
                     EIARAVATDPVLLLLDEPTAGMNSQETGEIMEIIMRLQKLGKTVLLIEHDMKVVMGIS
                     ENIVVLDHGVKIAEGVPGAIKRDQMVIEAYLGRQEHAQA"
     gene            complement(7975..9093)
                     /locus_tag="VMU21_10630"
     CDS             complement(7975..9093)
                     /locus_tag="VMU21_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78016.1"
                     /translation="MLRSVLIALWLSLLFLPFRGLKASLILFVILSCSFIVFKYLGAP
                     LRSLRATMQPLGNRVYTKGIALSRLKGSRYLVLLILLGLPFVLENYVLDISILTGIYA
                     ILAIGLNVVVGFAGLLNLGYVAFYAIGAYTYALLYAKMGLGFWVSLPLCIGLTTLSGF
                     VLSVPALRLRGDYLAIVSLGFGEIVRLILNNWDSLTNGPNGISGIPVPSVLGFSLGGL
                     NRYYYLILFCVILTYGVVKRIRSSKIGRAWMAIREDEICASTMGINTTMYKLYALSFG
                     SLWAGLAGALFAAKMQFVSPESFTFMESVLILCMVILGGIGSLPGVVAGAFILIMLPE
                     VLREVQSYRMLALGVGLILMMVFKPQGLLGGPGSRSNE"
     gene            complement(9098..10000)
                     /locus_tag="VMU21_10635"
     CDS             complement(9098..10000)
                     /locus_tag="VMU21_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUO78017.1"
                     /translation="MFFQQLINGLTVGGVYALIALGYTMVYGILELINFAHGEIYMLG
                     AYLGIIFFGFFTASGLTSFSLLLSLLLTFAMAVIFCSAYGFTMEKIAYRPLRNAPRLS
                     PLISAIGVSIFLQNYVMLTQGATDKVFPHLFGTSGFTLFNARVTHLQTAIVLVSLFLM
                     VSLHLFITKTKMGKAMRAVAQDKAMASLLGINIDSIISLTFIIGSGLAAIAGIMIALY
                     YGLVNYSIGYIAGIKAFTAAVLGGIGSIPGAMFGGLFLGFVESMGASYISSEYKDAYA
                     FLVLIVVLLIKPSGIFGKTTEEKV"
     gene            complement(10061..11179)
                     /locus_tag="VMU21_10640"
     CDS             complement(10061..11179)
                     /locus_tag="VMU21_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HUO78018.1"
                     /translation="MTKKLLTLCATFTIMLVGVGGCQKKGEIIKIGVAAPMTGDQAKI
                     GTDLRNGVTLAVEEWNAKGGVLGKKIEVVAVDDQHDPKQAVSVANKLVNERVAGVVGH
                     FNSSCSIPSSDVYNRGGVPMISPGSTNPQLTEKGYRGVFRVCGRDDQQGKVGAEFVIN
                     KLNLKRIAVIHDKTTYGQGLADEFRKFVKGKVEIVYYGGIIQGDKDFKTVLTSVKEKN
                     PQLIYFGGIYPEAGLLVKQARELGMTVPLMSGDGTIDPKFIEIAGVKAAEGTYLTFSP
                     DTRQILTAKAFLENYEKKYGEIGPYSVYAYDAANILLAAVREAGNSEGKAVMDKLHSM
                     EFSGALGKIQFDEKGDVTVSPYVVWVTKDGNFIEYWRP"
     gene            complement(11271..11978)
                     /locus_tag="VMU21_10645"
     CDS             complement(11271..11978)
                     /locus_tag="VMU21_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO78019.1"
                     /translation="MNNMSGGSDSREKKRVLVWEEDILINGIIKANALDISENGMYIV
                     TESDLPSGTVFDMAFKIHGTPIKVKAMVQHSQPGVGMGVKFLELTHAQLLLIRKYVDQ
                     VKEIAAKKAERKILLMDDDAQSRVAFRSKLSLEGYIVIEASNGVETLKHLQDLKPDLV
                     IMDLWMEGMDGFKILQLMRQNSELKNIPVIILSARTIPVDIHRATALGASDFLPKATT
                     TPLNLVEKVRELFSSLG"
     gene            complement(11971..12615)
                     /gene="thiE"
                     /locus_tag="VMU21_10650"
     CDS             complement(11971..12615)
                     /gene="thiE"
                     /locus_tag="VMU21_10650"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HUO78020.1"
                     /translation="MIKSSLLKEVPPFLGGFCLVTDRKLCGLTCEEMTRIALEAGAGW
                     IQYREKEKTRREIYGEAIRLRELTTEYGAALVVNDHTDIARAVGADGVHLGQDDLPLK
                     EARRIMGDRIVGISTHSLPEAMDAERNGADYIGFGPVFVTTTKDAGEPRGLEMLGRIR
                     KAVKIPVVAIGGISEESLPLVLNAGADAVAVASAVLRGDISENVRSFMRIIRDE"
     gene            complement(12602..>13162)
                     /locus_tag="VMU21_10655"
     CDS             complement(12602..>13162)
                     /locus_tag="VMU21_10655"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HUO78021.1"
                     /translation="KNVGTVEFIVDAAGDIYFMEVNTRVQVEHPVTEAVTGIDIIKEQ
                     IKLASGLPLEYRQSAIKPSGHAIECRINAEDPERFIPCPGKITFFYQPGGPGVRVDTA
                     AYSGWAVPSQYDSLIAKLIVHDTTRKDAIEKMKRALNEFIIEGIKTTIPFHKKVFSHK
                     DFISGEFDTGFAEKVSQPEGTLNDKE"
BASE COUNT         3207 a         3520 c         2870 g         3565 t
ORIGIN      
        1 tccctcactt ttccgtttgc ctttttctgc atattacgat atcttttctt catagaaagc
       61 gatctccttt tggtttttgg tgtggtaatc aattctaatt ggggatcgct tcctacttca
      121 acaatttaca ggggcatctt caatatttta tcaggcagag gttactatga cattaaggga
      181 atgttaagat tctgttacag taggaatgtg accaatcctt ttaatcatcg gtgaacttaa
      241 ggcgccactg aggtctataa atcaattgga ctgagaccgg ttctctttgc aatccgagcc
      301 agcatacgcg gccctatctc atcattatca tgaaaggcga acacataatc agggaatcct
      361 gaccttgaaa ggacacggtg ggaaccagcg gtatctcttt ttatgtacca gccaatgcga
      421 aggagtgcgg caaatatgtt acgtgccttg caagatggcc attggctcat gcagacagcg
      481 caaaaagctc atcaagttcc ggaatgctct caccatgatc cagtcgatca gcaataacgc
      541 gcagggccag tgcttcaact ttggcaattg cttcatcgcg tgtcttgccg taagccaaga
      601 cacccggaag atcagaaacc tcggcaatcc agcgaccgtc atcttccttt tcgatctcta
      661 ttttcatatc attcacctct ggtaataaat taatcagaaa acaagattat tttcaagtca
      721 aaaaatacgg aaggcaaaag atcaacacaa ctttggccat attctactgg gatgtgtccc
      781 taaataatgg caattaatct cttaatcaag gggtgcttat gatttattgt ctgttccact
      841 gaacagagag ttcttgccgg gcgaaactat tgagggcttc cgaaagaaag gtggagtaac
      901 aatggccatg agcagccggg ccacaaacga caagagtaac aaaagcccgc caggcttact
      961 cccgatcggt ctcgatggca gtgttgtacg ctatttaata gtatttttat tttccgttcc
     1021 gcgtcagaaa tcatatatct tccaaactcc tttggcagtc tccattaaat tgataggata
     1081 ggcgaatatc tcaccatttt ctttcttagt aagttcgcac tctgccaaat ggtcacttac
     1141 ccacctaatc tccacaccca tataaccttc aagtgcggcc ttcagagtat tattgtcttt
     1201 cagtgcctta aatatttgtc tatgtttatc tttcctcgac tcaacaaaaa aggctaacgc
     1261 agcctctata ttacctgctc gcaattgaga gatcattgct gccactattc tgttgaattc
     1321 ctgttctata cttttctttt ccgattcact gattttatct tcagatgcgg gaacagcgcc
     1381 ttcacggatt ccagtatctt cgctcgaact gctcatctct gttttgttcc tatcgtgcgg
     1441 cgtaacatca ggctcagata ttttcttata tttttccaag tcctgatcag taaaggttgg
     1501 tatgtcctgc tgtattattt ccccagcaaa tacgggagga ataagaataa gattccataa
     1561 aagcactgca attttcttca tcgtaaccct ccttatatat gtctcggaga gcactggtta
     1621 cttctccctc atatcctccg agtactcgtt ttcccaatta ttatcttatt cgtatgaatg
     1681 agttcacacg ccgcgaagcg ggcggcgtgg aacgacaatg ttagacttct gtctcgacta
     1741 ttgtatcacc tgatttgggt ggctgggcta ccgctgacgc cgctgttaca acctcaaggc
     1801 gcagggtgca ccagctcttg aaaacccgtg aagcttaatc cctgtcgcct cgatgttgtt
     1861 gttaactggc ggttcgtatt tgtcatttat tgtatttgat cccagtctta ctctcgtaat
     1921 taagatcttt gtatggggtt ttgactttgc agcgagccat tttgggatta ctggcaaata
     1981 tctcagcaaa ggttttctgg tccgttattc ttataattaa attatcatgt tcgattgttg
     2041 catcaatttt catttctgtt tggacccagt ttgacgcgtc ccacacggat ttctgcgagc
     2101 tggatatcag ttccctaatg ttcccttttt tctcagaacg gtcgaacttg aaatgaagaa
     2161 cgccaaacct atacttacac tgaatatcgg accgctctaa acaaagctcc caagaatatt
     2221 ccataacgtc gtctgatgtt tcattcatgt cccaagtcag tgtttgtcct tggggtatag
     2281 gaaaactaaa ttgtgcagtt tcgccgttaa ctacggcagt acatttcttt tcgtatgccg
     2341 gagcctgagc tgcgcctacg ttacaaagtg agagtgagat cagaatgacg agtccgatag
     2401 ttttcattat ctattatcct ttgcagttaa cacgcgcctg acgcgccgct tttggcgcgt
     2461 cgctgtccag gcgtaggtta tctctcgtgt attgcaggca gttattattt cttggatgtc
     2521 actcctttta tgtaagcttg aaaatcagat gattcaaaaa tatcccatat atatgatatc
     2581 gggacaacga ccgatatttt cccgccggtt tgatcggata atgcgctact ttgcacgcct
     2641 aacaagtgca atatttctcc acctttcatt tgctgattac cgatcataac tcctgacata
     2701 acgtcttcaa cataaaaaac cggagcgccg ctgtaacctt gagagagggc ttgatcaaga
     2761 agatagaagt gaaggtcagc gggaatgtct ggattagcaa tagatgtcag tttgctcgca
     2821 atctttgctt ctttcgcaac agggctaagt ttatctaaaa cacctaagcc caaggggaat
     2881 ccaacaatat atgcgggcgt aagtagttta atgtctgcat cctttttcgg aaacaaatcc
     2941 tcgggtagcg ccgccgcatc cacagcgcca ggatagccca taggatggat agagatatct
     3001 gctttaggat gatgaaacca atgtgccctt ttgataatat tagcgtcaga cagttgctgg
     3061 aaggttatga tggtagtatt gccgtttgga agattaatga taatttcgcc ggtggataaa
     3121 aggttttttg caacgtgctt ggccgttacg agataatctc tgccgatata tttaattaaa
     3181 aagcccgtcc ctagtgatgt ttctattatc ggcttatatt ccttactgtc agggtaacga
     3241 tagtacagcc cggcaggttt gttatccatc ggtatcattt tctgggattg ctgccgtaag
     3301 aaaacaacag ttttgttaag gtcatcgatc gacactgcaa gagcagatgt gaaagaaaat
     3361 agaattataa aaatgacctc gaacagcatt ttcattttgc gtttccatct ttttcccatt
     3421 tttgcgcctt ttgccaccct aagataatgc ggaactaagc tgcccccgaa aggggtctgt
     3481 cttgagtgac tggttctccc cgcgcttcgc gggcagtcac gacagtttag ttccgcattc
     3541 cctccgcgcg aagcgcgggg ggaaccagtt attagatagt ttcttgctaa tactccttct
     3601 tgtggtgtta gtcagccaaa caataccgga ttttttatat tagtcaggta aaactcaact
     3661 ttccccatcg ttgatctcca gataataact tacacttttc ccaggacttt gaaaagagtc
     3721 ggcttcaaaa aaaaatgaat tgtcccttat tatgctgatg ggctattttc tgaggtcaaa
     3781 atcttcacct ttgtccccct gccctgcgtg ctctcgattt ccatattcca tccatgggcc
     3841 tttacaagat gttttacgat agcaaggccg aggccagtgc ctcccagctc ccgtgaacgg
     3901 gccttgtcaa cgcgataaaa cctttcgccg agtctcggca gatgctttct gggaatccct
     3961 gtccctctat cttcgacaaa aagtattgtg tgctctccat ctttttccat tcccacagtc
     4021 actccccctt catcggaaaa ctttatgccg ttgtcgacaa ggttgaggag aacctggatc
     4081 agtttatccc tgtcagcgct tatctcaaag ggctctggag ggagggcttt cttgagaaat
     4141 aacccttttg cctctgcctt gtccctgaga gtggtgaaca cagtatcgat aatattattg
     4201 atattgacat tgtgcttatg caaatcgata tctcccatct ctatcctgga aagggtgagg
     4261 aggtcgctca caagggaatt cagccgctcg ctgtggttct ttatggtctc cagaaatttg
     4321 cgggcattct ctttgtcgtc aatggcccca tccagaagcg tctctgcaaa accctttatt
     4381 gcggtgatgg gggtctttat ttcgtgagag acattggcca caaaatcctt cctcatttcc
     4441 tcaaggtgct tcagccgtgt aatatcatgg agtgttatca cgacccctga cagcttctca
     4501 ttcactgaat acgcataaaa gggagttgac gttatcatca gggagagttc cctgggatgg
     4561 gcgacggaaa tctcatgcga taccggccct tcttccctga tcacctcatc tatcacctcc
     4621 atcaattctg cgttcctgag tgactctgcc acagtgcttc cttcaatctc ggtctcgatc
     4681 gcgaagagtt tcctcaccgc ctcgttgcaa agcagtattc tgccttttgt atcaaccagc
     4741 ataagtccgt cggagagatt cctgaggatc gcctccacct tgtgcctctc ctcctcgctc
     4801 tgcttcagcc ttgccttcat ttcatgtgcc atgtcattga tgttctttgc aagttcacct
     4861 atctcccctt tttcctttaa aaaaagcctc cgtcccagat ggcccgtggt aacctcccgt
     4921 gaaaaggccg tgatctcctc gatggatttg gttatcctcc ttgtctggaa aagccccatc
     4981 aaaatcgcaa tgaacaggac cgcaaaggaa gcgaagagca cactcatacg ggtctttgcc
     5041 accgcaactt ccacctcatg gaggggcatt gataacctga gaaatatttt ttctgagccg
     5101 tgtattaccg gaatggcaag atagaagagg ttctttccaa gggtcttact gaacctgatg
     5161 gaactgcctg cattgcctat agccgcatcc ctgatctcgg gcctgtcagc atgattctcc
     5221 atttttgcgg aagactcatc agagtcgcca atgacccggc ctgaactatc aatgatggtt
     5281 atccttgccc ctgtcttttc cttgtacttc ttgcagaaat cgtccagatc cacaggcagg
     5341 gtcaggggga tctcctctgc gataagattc ccctggacta agagactctg cttcagctcg
     5401 gatacgtggg tgctcttcac tacattggag agataaagct ctaggaagag aaagaggagc
     5461 ggcgcaataa tgaggtatga gacaaggatt cttctgaaga ggatgcgttt cattccttct
     5521 ctgcgaagaa atatccgatg ccccggaggg ttttgatata cttcggattt gcagggtctt
     5581 cctcgatctg ggacctcaat cttcgtatat gcacatccac tgtcctgggc tccacaaatg
     5641 cctcgtcact ccatattgcg tcaagaagct gctccctgct gaataccttt ccctttctct
     5701 ccacaaggta gaggagaagc ttgaattcag ttgcgctgag tttgaccggt tttcctttga
     5761 tggaaacgcc atatctctcc ctgtcgatct caagccctcc tatcttcagc atcccctctc
     5821 cggaaggcct ctccccggga gacctcctca atactgcctt caccctcgcc acaagttccc
     5881 ttggactgaa gggctttgtc atgtagtcat ccgctcccat ctccagcccg aggaccttgt
     5941 ccacttcctc cccctttgcc gtaagcatga tgataggcag accagaagtc ttgggatcat
     6001 tcctgagtat cctgcagagc tccatgccct ggaggccggg cagcatgagg tccagtatca
     6061 ggaggtcata ttttttcttt ctgatttttg aaagcgcctc ctccccgtct gatgctgaat
     6121 ccacggcaaa gccctctttc ttcagattat aagagacaag ctcgacaagg tcagcctcat
     6181 cgtctaccac gagtacattc cgtgaattca tcagtagtac cttatctgga acccctgcag
     6241 gtctccttta tagtcctccc atgcctccac gacatcctca acccctgctc ccggaggacc
     6301 catgcggcaa tgttttatag cctgctctat atcttccctg ttgccctcaa aaaccgcctc
     6361 cacactgcca tcagagagat tccttaccca tcccctgatg ccaaggtgac ttgcgatatc
     6421 acgggtgaat cccctgtaat acactccctg caccctcccc ctgatcaaaa gatgtaccct
     6481 tgccagctgc atacgctgat tataccagaa atcacttcat tcacccaggt aggctttttt
     6541 cacctgctca ttcctgagaa gttccacgcc ggtgccctgc gcgcttatct ttccgctttc
     6601 caatacatac ccttttcctg agaggttcag ggcagcgtgg gcattctgtt ccacaagaag
     6661 gatggtcatt ccctcatcat ttatctcctt tatggtcttg aaaatcctgc tcacaataat
     6721 cggcgccagg cccaatgagg gctcatcaag gagaagcatt ttgggatttg acatgagggc
     6781 cctgccgatg gcgagcatct gctgctctcc gccactcagg gtcccgcccg gctgcttctc
     6841 cctttccttt aagatgggaa agagtgaata caccctttca tagttcgctc tcgttttgct
     6901 gctgtgcagg aatgcaccca tttcaaggtt ctcccttacc gtaaggcggg ggaatatcct
     6961 ccttccctca ggaacctggc ttatccccaa cttcacaatc tcatggggcg ggagattgct
     7021 gatcaccctg ttttcgaaga gaatccgtcc tcccctcggc ttgataaccc ctgagatggc
     7081 catgagggtt gttgatttgc ctgccccgtt attccctatc atgcaggcta tttctccctg
     7141 gcctaccccg atctccactc ccctgagcgc ctcggcattg ccatagaagg tatggacatc
     7201 ttcaagcctg agcatgttcc tgccttccca gatacgcttc gatcaccatc tggtccctct
     7261 tgatagcccc ggggactccc tccgcaatct tcaccccatg gtcgaggacc acaatgttct
     7321 ctgaaattcc catgaccacc ttcatgtcat gctcgatgag gagaaccgtc tttcccagtt
     7381 tttggagcct cataatgatc tccatgatct ctcctgtctc ctgggaattc atccctgcgg
     7441 tcggttcatc cagcagaaga aggacagggt cagtggccac tgcccttgcg atctcgaggc
     7501 gtttctgacc accatagggc aggttgagag agggaatgtg tgcagcttcg cagagaccaa
     7561 cgaagtcgag atactccaag gccttttccc gtgcggccct ctcctctgaa agaaatgccc
     7621 tatccttcag gagggttccg aaaatcccgg tgttgatctt acggtgctgg gaaatgagca
     7681 cattttccag aacggtcatc tccctgaaga gccgtatgtt ctggaatgtt cgcgcaatgc
     7741 ccagtttgac gatttcatgg gaagggagtc ccgtgatatc tgtcccggag agggagaggg
     7801 aaccttttgt gggcttcgtg attccggtaa gacaattgaa gagggtcgtc ttgcctgcgc
     7861 cattaggtcc aatgatactt gcgatggagc ccccttctac tctgaagctc acatcttcaa
     7921 tagcctttac gcctccaaaa tgcttcgtta atccccgaac ctcaagcagg tacattattc
     7981 gttgctcctg ctccccggac cacccagaag tccctggggc ttgaagacca tcatgagaat
     8041 caaccctact ccaagggcaa gcatcctgta ggattgtact tcccttaaga cctcgggaag
     8101 cataataagg ataaaggccc ccgccaccac accgggaagg ctccctatcc ctcccagtat
     8161 caccatgcaa aggatgagca cagactccat aaaggtaaaa ctctcagggg acacaaactg
     8221 catttttgcg gcaaaaaggg cacccgcaag tccggcccag agagacccga aggagagggc
     8281 atagagttta tacatggtgg tgtttatccc catggtggac gcacagatct catcctccct
     8341 tattgccatc catgccctcc cgattttcga ggaccggatg cgcttcacca caccataggt
     8401 cagaataacg caaaaaagaa tcagatagta atacctgttc aatcctccca aggaaaaacc
     8461 gaggacagag ggtacgggaa taccggatat gccgttgggc ccattggtca gggaatccca
     8521 attgttcagt atcaaacgga caatctctcc gaaaccgagg ctcacaatag caaggtagtc
     8581 cccccttaac ctcagggcag ggacagaaag cacgaagccc gaaagggtgg tcagtccgat
     8641 acagaggggc aacgataccc agaagccaag ccccatcttt gcatagagca gggcataggt
     8701 atacgccccg attgcgtaaa aggccacata gcccagattg agaagaccgg caaaacccac
     8761 taccacattc agcccgatgg cgaggatggc atagatgcct gtgagaatgg atatgtcgag
     8821 cacataattt tccaaaacga atggcagccc aagaagtata aggagaacta aatagcgtga
     8881 gccttttaat cgggagagtg cgatcccttt tgtgtatacc ctgttcccaa ggggttgcat
     8941 agtcgcgcgc aatgaccgaa gtggagcccc aaggtatttg aatacgataa acgagcatga
     9001 gagaatgaca aaaagaatga gagaagcctt cagccccctg aagggaagga agaggaggga
     9061 aagccagagg gcaatgagga ccgatctcag cattgtctca caccttctcc tccgttgttt
     9121 tcccaaagat tcctgaaggc ttgatgagca gtactacgat gaggaccaga aacgcatacg
     9181 cgtccttgta ttcgctcgaa atatagctcg cccccatgct ctccacaaaa ccgaggaaca
     9241 gccctccaaa cattgctccc gggatgcttc cgatcccgcc cagcactgca gcggtaaatg
     9301 ccttgatccc ggcgatatat ccaatggaat aattcacgag gccataatag agtgcgatca
     9361 tgatccctgc gatggccgcg agacctgagc cgataataaa ggtcaatgag attatggagt
     9421 caatattaat gcccaggagt gacgccattg ccttgtcttg tgccaccgca cgcatggctt
     9481 tccccatctt cgtttttgtg atgaagaggt ggagggacac catgaggaat aatgagacga
     9541 gtacaatcgc agtttgcaga tgggtgaccc tcgcgttaaa cagggtaaac cctgaggtgc
     9601 caaagaggtg gggaaatacc ttatccgttg ccccctgggt gagcatcaca tagttctgga
     9661 ggaatatgga tacgccgata gcacttatga gaggactgag acgtggcgcg tttctgagcg
     9721 gccggtatgc aatcttctcc atggtgaatc catacgcaga gcagaatatc actgccatgg
     9781 caaaggtgag caggagggac aacagaaggc taaaagaggt taggcccgaa gcagtaaaaa
     9841 agccaaaaaa gatgatgccg aggtaggcgc ccagcatata aatctcacca tgggcgaaat
     9901 tgatgagctc aaggattcca tacaccatcg tatagccaag ggctatgagg gcatagacac
     9961 ctcccacagt aagaccattt atgagctgct gaaaaaacat ggggaaacga ggaattaccc
    10021 ttcaacaaag gagactatca aaaccatgga gagactgtaa ctacggtctc cagtattcaa
    10081 taaagtttcc gtcctttgta acccagacca catagggcga aacggtcacg tccccctttt
    10141 catcaaattg tatctttccc aatgcccctg aaaattccat agagtggagc ttatccatca
    10201 ccgccttgcc ctcgctgttc cctgcttccc tgactgcggc aagaagaata tttgctgcat
    10261 cataggcgta taccgaataa ggaccaatct ccccgtactt cttctcataa ttttccaaga
    10321 acgcctttgc ggtgaggatt tgtcttgtgt caggactgaa ggtaaggtag gttccttcgg
    10381 cagcctttac ccctgcaatc tctatgaact tcgggtctat agtaccgtca ccgctcataa
    10441 ggggaaccgt catacccaat tctctcgctt gttttaccag caggccagcc tcagggtaaa
    10501 tacccccgaa atagataagc tgcgggttct tttcctttac cgaggtgagg actgtcttga
    10561 aatccttgtc tccctggatg attcccccgt aatacacaat ttcaaccttt cctttcacaa
    10621 acttcctgaa ttcatcagca agtccttggc cataggtggt tttatcatga atgaccgcaa
    10681 tcctcttgag gttcagcttg tttattacaa actcagctcc cactttcccc tgctggtcat
    10741 ctctcccgca tacccggaat acgccccggt atcccttttc ggtgagctga gggttagtag
    10801 aaccaggcga aatcatgggg acccctcccc gattatacac atctgatgaa gggatggaac
    10861 agctggaatt gaaatgccca acaacacccg ccactcgctc atttaccagt ttgttcgcta
    10921 cagagaccgc ttgtttagga tcgtgctggt catccactgc caccacttcg atcttcttgc
    10981 ccaaaacgcc tcccttggca ttccattcct caactgcaag ggtcaccccg tttctcaagt
    11041 cagttccaat cttcgcctga tctcctgtca tgggcgcggc aacaccaatc ttgataatct
    11101 cccctttctt ctggcacccc cccacgccta caagcattat cgtgaacgtt gcgcataatg
    11161 tcagcaactt cttagtcatc tcgtcctccc ttaaaggaat accattccta tgattcgtat
    11221 ctcttcacct aagtgcgaaa ggaaagagag gcctgcgtat tgatcccgca tcaacccaat
    11281 gaggaaaata gttcccgtac cttttccaca aggttcagcg gcgtagtggt tgcctttggg
    11341 aggaagtcac ttgccccaag cgccgtagcc ctatggatgt caaccgggat ggtccttgct
    11401 gaaagaatga tcacgggaat attcttgagt tcgctattct gccgcataag ctgcagtatt
    11461 ttaaacccat ccataccctc catccagagg tccataatca ccaggtcagg tttcagatcc
    11521 tgcagatgct tcaatgtctc gactccgttg gacgcctcaa tgacgatata cccctcgaga
    11581 gagagtttgc tcctgaatgc gaccctggac tgggcatcat catccatgag gaggatcttc
    11641 ctttccgcct tctttgcggc aatttccttc acctggtcaa catacttcct gatgagaagg
    11701 agctgggcat gggtcaactc aaggaatttc acccccatac cgacacctgg ctgggagtgc
    11761 tgtaccatgg ccttcacctt aataggcgta ccatgaatct tgaacgccat atcaaaaaca
    11821 gttcccgagg gaaggtccga ctcagtgaca atgtacattc cattctcact gatgtccagg
    11881 gcatttgcct taatgatccc gtttatgaga atatcctctt cccataccag cacccttttc
    11941 ttctccctgc tgtcgctccc tcccgacata ttattcatcc ctgataatcc tcatgaacga
    12001 ccgcacattc tcggatatat cacccctgag caccgcagat gccacggcaa ccgcgtcagc
    12061 tccggcgttc aaaaccaggg gaaggctttc ttcagatatt cccccgatgg ccactacagg
    12121 gattttcacc gctttcctta tcctgcctaa catctccagt cctcttggtt caccggcatc
    12181 tttggtggtc gtaacgaaaa ccgggccaaa gccaatatag tcagcaccgt tgcgctcagc
    12241 atccattgct tcgggaaggg aatgagttga tatacctaca atcctgtctc ccattatcct
    12301 gcgggcttct ttcagtggca ggtcatcctg ccccaaatgt actccgtccg cacccaccgc
    12361 acgagcaatg tctgtatggt cattcaccac taacgcagcg ccgtactcgg tagtgagctc
    12421 tctcagtctg attgcctcac cgtaaatctc ccttcgcgtc ttttctttct cccggtattg
    12481 aatccagcct gctcccgcct ccagggcaat ccttgtcatt tcctcgcagg tcaatccgca
    12541 gagtttccga tccgtaacaa ggcagaatcc gccaagaaaa gggggcacct ctttcaagag
    12601 actactcttt atcatttagg gttccctcgg gctgggaaac cttctccgca aaacccgtat
    12661 caaactcacc acttatgaaa tccttatgac tgaatacttt tttgtgaaaa gggatggtgg
    12721 tctttatccc ttcgatgatg aactcattca gcgcgcgctt catcttctcg atggcgtcct
    12781 ttcttgtggt gtcatgcaca ataagctttg cgataagaga atcgtattgg gaaggcacgg
    12841 cccacccgct gtaagccgcg gtgtctactc tcaccccggg tcctcccggc tggtagaaaa
    12901 aggtgatttt gccaggacag ggaatgaacc gctcagggtc ttccgcgttt atcctgcatt
    12961 caatggcatg gcctgatggc tttatggcgc tttgcctgta ttccagggga agacctgatg
    13021 ccaactttat ctgctctttg atgatatcta tcccggttac ggcctcggtc acgggatgtt
    13081 caacctgcac cctggtattc acctccataa aatagatatc cccggccgcg tcgacaatga
    13141 attcaacggt gcccacattc tt
//
LOCUS       DATKOU010000221         9813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4156146, whole genome shotgun sequence.
ACCESSION   DATKOU010000221 DATKOU010000000
VERSION     DATKOU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4156146"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VMU21_10660"
     CDS             complement(<1..663)
                     /locus_tag="VMU21_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78022.1"
                     /translation="MSTAFVYSDDFHRFNYGPSHPLKTFRLKLTYELIKAYGLLSIPN
                     TRLVEAVIADEDDLVTFHDREYIEILKSANSGIRVAGAGFYGLGSGDNPVFRGLFDWS
                     RLVTGASLQAAEMVDCGEVETAFNISGGLHHAMASRASGFCYLNDPVIAIMSLLKKGR
                     RVVYIDIDAHHGDGVQEAFYASNKVLTISLHETGSALFPGTGFEYETGEGEGQGYSVN
                     IPL"
     gene            complement(660..2282)
                     /locus_tag="VMU21_10665"
     CDS             complement(660..2282)
                     /locus_tag="VMU21_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HUO78023.1"
                     /translation="MNELPNMMDYEKEYRGPSLSAPERFSFPLDVFDGWGERTALCWT
                     DGVMEKKYSFNEMRMRSSKGAGALKRMGIRRGDKVLVMLSNVPEWWEIMLSLMRLNAV
                     PIPATTLLVSKDIEYRLSATEVKAVIASEEDASKVEDAVNGLSVTPLLILIDERQGWD
                     NYIKERDCADMVVGERGSAQEPAIIYFTSGTTGPPKMVVHTHASYPFAHQLTGKYWLD
                     LRPGDIHWNLSDTGWAKAAWSSLFGPWHRGATIFSLHRKGKFDPSFTLEVMQRYPVTT
                     FCGPPTAYRMMVKGASASQLFRFRSMRHFVAAGEPLNKEIIELWKEKTGRYIYDGYGQ
                     TETVNVLANFRCLPVKPGSMGRPVPGFVVDIIDEKGVPMPSETEGDIGIRVHPERPVG
                     LFSKYLGDSEATERTMKGQWYSTGDRAFRDHEGYFWFVGRDDDIILSSGYRIGPFEIE
                     SILIEHPAVRESAVVASPDEIRGEVVKAFIVLTQDYRPSDILAHELREFVKTNAAPYK
                     YPRKIEFVDDLPKTISGKIKRKELKMREYGMS"
     gene            2456..3079
                     /locus_tag="VMU21_10670"
     CDS             2456..3079
                     /locus_tag="VMU21_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HUO78024.1"
                     /translation="MTFSEHEVKGLFKEMGFAVPKGRFFARGEAILPPTDLTFPLVAK
                     ISSSKVSSKSDVGGVKTGIKDKDELSRAVQELMRVEGAEGVLVEEMAPQGVEVIVGGV
                     IDNQFGPVVMFGLGGVFVELFRDVAFGLAPLTPGDALWLVQQTKGHRLLKGYRGKPPL
                     DIAVLIHTIVAVSEIMATGMVKEIDLNPVALYPEGSLILDAKMAVIS"
     gene            3163..5292
                     /locus_tag="VMU21_10675"
     CDS             3163..5292
                     /locus_tag="VMU21_10675"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HUO78025.1"
                     /translation="MSEEEVTVDSLIKKVLSYNPDADVELLRKAYIFSREAHCSQRRI
                     EGTPYIEHPLSVADILTDMKVDVATIAAGLLHDTIEDTETTPRDIKDIFGEEIAFLVD
                     SLTKLSKIEFKTKEEAQAENFRKMLLAMSEDIRVIIIKFADRLHNMRTLQFLPENKRQ
                     RIATETLEIYAPLANRLGIGWLRLEFEDLSFKVLMPELYEELERKVVKRKEEQEGYLR
                     EVTKTVEDRLKEEGIHGRVSGRVKHYYGIYQKMQKQRITFDQVHDVLGLRIITDTNAN
                     CYTIMGLIHSLWTPIPGRFKDYIAMPKSNMYQSLHTSVIGPKGERVEFQIRTKEMHRI
                     AEEGIASHWKYKEKDAVRERDSKYISWLRELIQSQKELPDAREFLEAVKGEVIPEVVY
                     VFTPKGEIRELPVGSTPVDFAYSIHTQVGHKCVGAKVNGRIVPLRYQLKNGDTLEIIT
                     SPTHGPSRDWLKFVVTQRAKGRIKQWIKAEEMKQSIELGLKLLEVEMKKHHLKPSSLK
                     ADEMQAMAKAFSLQSFDDLLVSVGYGKVSAHQVVNRLLPEKEQEELVQRAARPEREQK
                     GITIKGIDHILYHTAKCCFPIPGDVLVGFVTRGRGVTIHRRDCPNLESLAIDDGRIVD
                     VDWKPGKETTTPARLLVETVDRAGMIADLSALISSVNVNISHMQATTTQDEKAHIIFD
                     LAVTDRLQLTGLIQKIAQTEGVLRVKR"
     gene            complement(5482..7425)
                     /gene="feoB"
                     /locus_tag="VMU21_10680"
     CDS             complement(5482..7425)
                     /gene="feoB"
                     /locus_tag="VMU21_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HUO78026.1"
                     /translation="MGQYDQIALIGNPNVGKSVIFGLLTGKYVTVSNYPGTTVEVSHG
                     NIALDSKKFHVVDTPGVNSLIPMSEDEKVTRDILLKERPSLVVQVSDSKNLKRTLLLT
                     LQIAEMGIPMVLDLNMEDEARDRGIIINVQKLKEILDTEVVTTIAPQRKGLKELRNSI
                     LNPSLPKVRIHYPPVIEEYVEKISLLLPEANISKRSVAVMILSGDESLKGWLTAHLPG
                     EVIKEMEALRDECQLKMHGTLSETVNRTRLELAGEIAEKVIKYIPPEGGQLFYHIGRL
                     SMHPLWGIPILLIVLLGLYEFVGVFGAGTLVNFFEKTIFGKYINPMATKVVQLLVPIG
                     FIQEMLVGEYGLVTVALTYAIAIILPITATFFIAFAILEDSGYLPRLAIMSNRVFSLM
                     GLNGKAVLPMVLGLGCGTMAVMTSRILEARRDRIIITFLLALAIPCSAQLGVILGMLS
                     ALSFFATIIWVGCILIVLFLSGYLASKVIKGERTDFFLEIPPIRRPAIANILIKTAGR
                     VEWYLKEAVPLFILGTFILFLLHKLNLLLILEKAASPVVVHLLGLPEKTTESFILGFL
                     RRDYGAAGLFTMAEKGLLTPVQSLVSLITITLFIPCLANFFMIIKEMGSRIAIAMAAI
                     VFPLAVLTGGALNWILTVLHVRL"
     gene            7751..8230
                     /locus_tag="VMU21_10685"
     CDS             7751..8230
                     /locus_tag="VMU21_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HUO78027.1"
                     /translation="MNRRAFLKTMTVGTMAMGMVERAFAAERYFPVKADQSLFENINR
                     VKDPSNKTPLEKSHAPFIKAPAKVKAGEPFTVEVSVGEVVHPMGPAHWIEFITLNIGN
                     EPAGKVDFRSKGYLNPKATFTVVLQKESAPSGKVTLIANQRCNLHGYWESSQDITVT"
     gene            8296..9141
                     /locus_tag="VMU21_10690"
     CDS             8296..9141
                     /locus_tag="VMU21_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HUO78028.1"
                     /translation="MSIGNYKGFLDALGLDEEPMGMYYTNQKPEGGFCPKKGTLPSAS
                     QEAKGEVDFGSLFENFSCVIGNIWLARRKKTLAYFDREHFGCLGGAFYLGFLKPQLDF
                     VAHYVSSGIPGHLEGEHYLESPEVTRSFFKAVDPAPAPARFCVFKPVSQFLGNDTPEV
                     VTFFARAEVIAGLNQLATFVTNDFEAVSSPFGAGCTNIVTWPLKYLSEGKLKAVIGGW
                     DPSDRKFLKTDEITFAIPYEMYKRMITRWPESFLNTKTWTMVKKKIALSKKTWGELPN
                     APSKQ"
     gene            9383..9580
                     /locus_tag="VMU21_10695"
     CDS             9383..9580
                     /locus_tag="VMU21_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78029.1"
                     /translation="MKKAELTLPEIALIAGTRGMLGAGAGLLLADKLNKDQRKAIGWT
                     LLIVGALSTIPLTMEVLNKRR"
BASE COUNT         2571 a         2451 c         2380 g         2411 t
ORIGIN      
        1 cagagggata ttcacagaat atccttgtcc ctctccctcc cctgtctcgt actcgaaccc
       61 ggttccgggg aagagcgcac ttcctgtttc gtgtagtgag atggtgagca ctttattgct
      121 cgcatagaag gcttcctgta ccccgtctcc atggtgggca tcgatatcaa tatagaccac
      181 tctcctgccc tttttcagaa gcgacataat ggcgatgacc ggatcattga gataacaaaa
      241 tccggaagcc cttgaggcca tggcatggtg aagcccccct gatatgttga aggcagtttc
      301 cacttcgcca caatccacca tttccgctgc ctgcaaggat gctcctgtca caaggcgtga
      361 ccagtcgaag agacccctga acacggggtt atcaccggaa ccgaggccgt aaaaccctgc
      421 accggccacc cttattccgc tgtttgcgga cttcagtatt tcgatgtatt ccctgtcatg
      481 aaaggttacc aaatcatcct catccgctat taccgcttca acgagtcttg tgtttggaat
      541 agagaggagg ccgtatgcct taataagttc gtacgtgagt ttcagcctga atgttttcag
      601 aggatgggag gggccatagt tgaaccgatg gaagtcatct gaataaacaa atgccgtgct
      661 catgacatcc cgtattccct catcttcaat tccttcctct ttatcttccc gcttatggtc
      721 tttggcaggt catccacaaa ctcaatcttc cgcggatact tatacggagc tgcattcgtc
      781 ttcacaaatt ccctgagctc atgtgcgagg atgtctgatg gtctgtagtc ctgagtaagg
      841 acgatgaatg cctttaccac ctctcccctt atttcatcag ggcttgccac aaccgctgac
      901 tcccttaccg ccggatgctc gatgagaatg ctctctatct caaagggtcc gattctatag
      961 cctgaagaga ggatgatgtc gtcatccctt cctacaaacc agaagtatcc ctcatgatca
     1021 cggaacgccc tgtcccctgt actgtaccac tgccctttca tcgttctctc cgttgcctcc
     1081 gaatctccga gatacttgct gaacagtccc acgggcctct cgggatgtac ccttattccg
     1141 atatctccct ctgtttcaga aggcatcggg acacccttct catctataat atccaccaca
     1201 aacccgggaa caggccttcc catggacccg ggtttcaccg gaaggcatct gaagttcgcc
     1261 aggacattca ccgtctctgt ctggccgtac ccatcataga tatacctccc tgtcttttcc
     1321 ttccaaagct cgattatctc cttgttgagt ggttctcccg cagcgacaaa atggcgcatg
     1381 gaacggaatc tgaatagctg agaggcagat gcccccttga ccatcatcct gtatgcagtg
     1441 ggaggaccgc agaatgtggt gacaggatac cgctgcatca cctccagtgt aaaggacgga
     1501 tcaaattttc ccttcctatg aagggaaaag attgtggctc ccctatgcca gggtccaaaa
     1561 aggcttgacc atgcagcctt tgcccatcct gtatcagaaa gattccagtg gatatccccc
     1621 ggtctgagat cgagccagta ctttcctgtt aactgatgtg caaaggggta tgaggcgtgg
     1681 gtgtgaacca ccatctttgg gggaccagtt gttcccgagg tgaaataaat tatggcaggc
     1741 tcctgggcag aacccctctc tcccactacc atatcagcac agtcgcgctc cttgatgtaa
     1801 ttatcccacc cttggcgctc atcaatgagt atcaggagag gagtaaccga taaaccattc
     1861 accgcatcct ccaccttgga ggcgtcctcc tctgacgcaa tgaccgcctt tacctcagtt
     1921 gccgagagtc tgtactcaat atccttggaa acgagaaggg ttgtggcagg aatgggaacc
     1981 gcattcagtc tcataaggct gagcataatt tcccaccatt cagggacatt ggagagcatg
     2041 acaaggacct tgtctcccct ccggatgccc atcctcttaa gcgcgcccgc accctttgat
     2101 gacctcatcc tcatctcatt gaatgaatat tttttctcca taaccccgtc cgtccagcaa
     2161 agggctgttc tctcacccca tccatcgaac acatccagag gaaaggagaa tctctcagga
     2221 gctgacagag acggtcccct gtattccttc tcgtaatcca tcatgttagg caattcattc
     2281 atcccaaaat ggtaacatat ttccacctat caggattccc cttctcatat cagaaaaatc
     2341 aggtatactt taaacagcta tgccaacgag caacgaaaac ccctccccct ccacaggccc
     2401 tgagagccct ttcacaaaaa tcctcaatga cttcatgacc aggaacagga ggcgagtgac
     2461 attttcggaa catgaagtga aaggactttt caaagagatg ggattcgctg ttccaaaggg
     2521 gagatttttc gccaggggag aggccatctt gcctccgacg gatctgacct ttccccttgt
     2581 ggcaaagatc tcttcatcaa aggtctcttc aaaaagcgat gtgggcggtg tgaagaccgg
     2641 gattaaagac aaggatgaat taagtcgcgc agttcaagaa ctcatgcggg ttgagggtgc
     2701 agaaggggtt cttgttgaag aaatggctcc tcaaggcgtg gaagtcattg tgggcggggt
     2761 gattgacaac cagttcggtc ctgtggtgat gttcggcctg ggaggggtgt ttgtggaact
     2821 tttcagggat gttgcctttg ggctcgcgcc ccttacgccc ggagacgcac tctggcttgt
     2881 ccagcagacg aaagggcaca gactcctgaa ggggtaccgg gggaaacctc ctttggatat
     2941 cgccgtattg atacatacaa ttgttgccgt ctccgaaatt atggcaaccg ggatggtcaa
     3001 ggagatcgat ttgaaccccg tcgcccttta tccggaagga tccctgatac tcgatgcgaa
     3061 gatggcggtc atttcctgac cccgctccct gctgtttttc ccttaagctt ttgcactaaa
     3121 gccaaatgca tagagccaaa acaaaagttg tataataaaa ccatgtcaga agaagaagta
     3181 accgtagatt ccctcataaa aaaggtgctc tcctataacc cagatgctga tgtggaactc
     3241 ctgagaaagg catatatatt ctcgagggag gctcactgca gccagcggag gattgaaggg
     3301 actccctaca tagagcatcc cctctcggtt gccgatatcc tcaccgacat gaaagtggat
     3361 gtggcgacca ttgccgcagg cctgcttcac gataccatcg aagatactga aacaacccca
     3421 agagacatta aggacatctt tggtgaggag atcgcattcc tggtggattc tctcacaaaa
     3481 ttgagcaaga tcgagttcaa gacaaaggaa gaagcacagg cagagaactt caggaagatg
     3541 cttctcgcca tgtcagagga tataagggtc attatcatta aattcgctga caggctccat
     3601 aatatgagga ctctccagtt ccttcccgaa aacaagagac aacggattgc caccgaaacc
     3661 ctggagatct atgcacccct ggcaaacaga cttggaatag gatggctcag gttggagttc
     3721 gaagacctca gtttcaaggt cctgatgccg gaactctatg aggaactgga aagaaaggtc
     3781 gtaaagagga aagaagaaca ggaggggtac ctgcgggagg ttacaaagac tgtagaggac
     3841 agactcaaag aagagggaat tcacggaagg gtttcaggaa gggtgaaaca ctactacggg
     3901 atctatcaga aaatgcaaaa acagaggatc acctttgatc aggtccatga tgtgctgggg
     3961 ctcaggatca tcaccgatac caatgcaaat tgctatacga tcatgggtct catacattcc
     4021 ctctggacac ccatccccgg aaggttcaag gactatatcg ccatgcccaa atcgaacatg
     4081 taccagtcac tccacacctc tgtcataggg ccaaagggtg aaagggtgga gttccagata
     4141 cggacaaaag aaatgcacag gatcgccgaa gagggaatcg catcccactg gaagtataag
     4201 gaaaaggatg ctgtgcggga aagggacagt aaatacatat cgtggctcag ggagttgata
     4261 cagtcacaga aggaactccc tgacgcgagg gaattccttg aagcggtgaa gggtgaggtt
     4321 atacccgagg tggtgtatgt ctttacgccc aagggcgaga taagggaact gcctgttggc
     4381 tcgacccccg tggattttgc ctatagcatc catacccagg tgggccataa atgcgttggc
     4441 gcaaaggtga acggcagaat tgtccctttg agataccagt taaaaaacgg ggataccctt
     4501 gagatcatca cctcccccac tcatgggccg agcagggact ggcttaagtt tgtggtcacg
     4561 caaagggcaa agggaaggat aaagcagtgg ataaaagccg aggaaatgaa acagagcatc
     4621 gaactggggc taaagctcct tgaggtggaa atgaagaaac accatctcaa accctcttcc
     4681 ctcaaagcag atgagatgca ggcaatggca aaggccttca gcctccagtc ctttgatgac
     4741 cttctcgtat cagtgggata tggaaaggta tccgcccatc aggtggtgaa cagactcctc
     4801 cctgaaaaag aacaggaaga gctggtacag agagcagcca ggccggagag ggagcagaag
     4861 ggcattacca taaaagggat tgaccatatc ctttaccata ccgccaagtg ctgttttcct
     4921 atacctggtg atgtccttgt gggattcgtc acgagaggga ggggcgttac catccacagg
     4981 agagattgcc cgaaccttga aagcctcgcc atcgacgacg ggagaatcgt tgatgtggac
     5041 tggaaaccgg ggaaggagac caccacgccc gcaaggctcc ttgtagaaac agtggacagg
     5101 gcgggaatga ttgcggacct gagcgcatta atatcatcag tgaacgtgaa tatctcccac
     5161 atgcaggcaa ctaccaccca ggacgagaag gcacatatta tttttgacct tgccgtaacg
     5221 gacagactgc agctcacggg tcttattcag aagatcgcac agaccgaggg cgtcctgagg
     5281 gtcaagcgat gacttatttt ttattcagtg tctgcgcgga tttcttttga atcctcgcct
     5341 tatccttatt tccttctggc ttcttcaatc cagcaataag taagacaatt gcgcccgccc
     5401 ccataaggac tctcattaca ggacctgatg caaaaagcac tcttttcaga atactcatat
     5461 ccgccctttc ctgatgattg ctcagagcct cacgtggagc actgtgagaa tccaattcag
     5521 cgctcctcct gtcaggaccg ccaggggaaa tacaatggca gccatggcga tggcaatcct
     5581 tgaccccatc tcctttatga tcatgaagaa attggcaaga caggggataa ataatgttat
     5641 cgtgatgagg ctcacgaggg actgtaccgg ggtgagcaat cccttttctg ccatggtgaa
     5701 gagaccggct gcgccgtaat ccctcctgag gaaaccgagt ataaacgact cagtcgtctt
     5761 ttcagggaga ccgagcaaat gcacaaccac aggcgaagca gccttttcca ggatcagcag
     5821 gaggttcagt ttatggagga ggaaaaggat gaaggtgccg aggataaaca gtggtaccgc
     5881 ttccttgaga taccactcca ccctgcccgc tgtctttatc aatatgttcg ctatggcagg
     5941 ccttcgtatg ggggggatct ccagaaaaaa gtctgtccgc tctcccttaa tcaccttcga
     6001 ggcaagatag ccggaaagaa agaggacaat gaggatgcat cccacccata taatggtcgc
     6061 aaaaaaggac agggcactga gcatcccaag gatcacccca agctgcgcag aacacggtat
     6121 ggcgagggcc aggagaaagg tgatgatgat cctgtctctt ctcgcctcca gtatcctcga
     6181 tgtcatgacc gccatagtcc cgcagcccag ccccagcacc atgggaagca ccgcctttcc
     6241 gttaagcccc ataagactga ataccctgtt gctcataatg gcaagacggg gaaggtaacc
     6301 gctgtcttcc agtatggcaa aagcaatgaa gaaagttgcg gtaatgggga ggattatagc
     6361 tatggcatag gtcagcgcca cggtgacaag gccatattcg cccacaagca tctcctgaat
     6421 gaagccaatg ggaaccagca actgcacaac ctttgtggcc atgggattta tatatttccc
     6481 aaagatcgtt ttttcaaaaa aattaaccaa tgtccctgca ccaaagaccc ccacaaattc
     6541 ataaagaccc aatagcacta tgagcaatat cggaattccc catagcggat gcatactgag
     6601 tcttccaatg tgataaaaaa gctgtcctcc ctcaggaggg atatatttta tgaccttttc
     6661 tgctatttca cctgctagct ccagcctcgt ccggttcacg gtttcactca acgtgccatg
     6721 catcttcagc tgacattcat ccctcagtgc ttccatctct tttatcactt ctcccggcag
     6781 atgagcggtt aaccacccct tcagactctc gtcgccagaa agtatcatga ccgccacgga
     6841 tctcttggat atatttgctt ccgggagcag gagggatatc ttctccacat actcttctat
     6901 tacagggggg tagtgtatac ggacctttgg cagagaagga ttaaggatag aattcctcag
     6961 ttccttaagt ccctttctct ggggagcaat ggtggtgacc acctcggtat ccagtatttc
     7021 cttgagtttc tggacattga tgataattcc acggtctctt gcctcgtctt ccatgttcag
     7081 atcaaggacc atgggaatgc ccatctccgc tatctggagg gtgagaagga gtgtgcgttt
     7141 gaggtttttt gaatcagaca cctggaccac aagggacggg cgctctttca gaagaatgtc
     7201 cctggtgacc ttttcatcct cgctcatggg aatgagactg ttcactccag gggtatccac
     7261 cacatgaaat ttcttgctgt cgagggcaat attgccgtgt gagacctcga ccgttgtccc
     7321 cgggtaatta gagacagtca cgtacttacc ggtgaggagg ccgaatatta cgctcttccc
     7381 tacattgggg ttgccgatga gggcaatttg gtcgtattgc cccatccttc ccccatgccg
     7441 atgtttatgt ttatgttcgt gcatgtgcat gacttagttg agacttagtc tcagttagaa
     7501 gtatatagga gctcgcacat ctctgtcaat gattacaatg gcagggtgcc caatttatag
     7561 ctaaataatt ggataccctg ttgatatata ggtattaata taaagtttct ttgattctta
     7621 cctcatggtg ttcccttgca actttcatgg caaagatatt ttcgaacttg aatagtgtcc
     7681 tgttatgtga tatgagaatt aaaggagcca tttactttct tactaatctg ttaacaagag
     7741 aggagggagt atgaacagaa gggcttttct gaagactatg actgtgggga ctatggcaat
     7801 gggaatggtt gagagggcat ttgctgcaga aagatatttc cctgtgaagg cagatcagag
     7861 cctctttgaa aacatcaaca gggtcaagga tccttcgaac aagaccccgc tggagaagtc
     7921 ccatgcaccg tttattaaag cccctgcaaa ggtcaaggca ggtgaaccat ttactgttga
     7981 ggtaagtgtt ggcgaggtcg ttcatcccat gggaccagct cactggattg agtttataac
     8041 gcttaacatc ggcaatgagc cggctgggaa ggtggacttc cggtcaaaag ggtatcttaa
     8101 tcctaaggca actttcactg tggtacttca aaaagaatct gcgccctccg gcaaagtcac
     8161 cctcatcgcc aatcagcggt gcaaccttca tggctactgg gaaagcagcc aggatattac
     8221 cgttacataa agttttttca ttttattgac agatactcca tgtcgtgcaa gtaaggagga
     8281 ttcgctatca aggagatgtc aatagggaat tataagggtt tcctggatgc cctggggctc
     8341 gatgaagaac caatggggat gtattatacg aatcaaaagc cagagggagg tttttgtccc
     8401 aaaaagggaa cacttccttc agcctcacag gaagcgaagg gagaagttga ttttggttct
     8461 ctatttgaga acttctcctg cgttatcggt aacatctggc ttgcccgcag aaaaaagacc
     8521 ctcgcttatt tcgacagaga gcatttcggc tgtctcggcg gtgcctttta tctcggtttt
     8581 ctgaaacctc aactggattt tgtggcacac tatgtttctt ccggtatccc cggccatctg
     8641 gagggtgagc attatctgga atcaccagag gttacccgca gtttcttcaa agctgttgat
     8701 cccgctccgg ctccggcacg tttttgcgtc ttcaagcctg tgagccagtt tttgggaaac
     8761 gatacgcctg aagtagtgac cttttttgcg agggcagagg tcattgccgg gctcaatcag
     8821 cttgccacat tcgtgaccaa tgattttgag gcagtttctt ctcccttcgg agccggctgt
     8881 accaacatag ttacatggcc gctcaaatac ctatccgagg ggaaactcaa ggcggtgata
     8941 ggaggatggg acccctcaga ccgaaaattc cttaagaccg acgaaatcac ctttgccatt
     9001 ccctatgaaa tgtacaagcg catgataacc cgttggccgg aatcctttct gaacaccaag
     9061 acctggacca tggtaaagaa gaagattgcg ctgagcaaga aaacctgggg agaactgcca
     9121 aacgcgcctt caaaacaata aaccgaaacc tcatcgggat gagtaatcgt ccctctcatc
     9181 tgtctctgct tcttcatgaa tgcgcaagga ttgaccgtgc tgccctagag acaacgcgtt
     9241 ttgggagttc acccattgcc ggcccttacg cgaagtgtat attttcatcc ggcaattgat
     9301 ataatttcta taagacataa aagtttttgg agaaacaaag acacaggtct ctgaaaccaa
     9361 ttgatagatt ttaggaggaa gcatgaaaaa ggcagaactt acattgccgg aaatcgcctt
     9421 aatagccggc acacgcggaa tgctgggtgc aggagccggt cttcttttgg cagacaaact
     9481 caataaggat cagagaaagg ccatagggtg gacactgttg atagtcggag cgctgagcac
     9541 gatccctttg acgatggagg tgctgaataa gaggcggtag aaatctctca ctataccctc
     9601 tcattcgcct tggcttcttt gggagagaac attttggcat tggtttcgcc cccaaacatg
     9661 caccgagata ttcttccttg tttattccag tcaagatatt tttatatggc tttttagtgt
     9721 cacttcccgt aggcggatat tccccatctt tgatgtgaca ttctacccaa cccttgtcat
     9781 tagaaagtcg ctttgaacgc ttttaccttg ctc
//
LOCUS       DATKOU010000222         4245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2624510, whole genome shotgun sequence.
ACCESSION   DATKOU010000222 DATKOU010000000
VERSION     DATKOU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624510"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..984)
                     /locus_tag="VMU21_10700"
     CDS             complement(229..984)
                     /locus_tag="VMU21_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO78030.1"
                     /translation="MKLKRAFRVWQRNFTVYTKLYKSSIALNFVEPVLYLLALGFGLG
                     AFVKDINGVPYMNFIAPGIIASSSMFAAIYECTYGTYVRMTFQKTFDAILATPVNLND
                     LVAGELMWGATKSMLYGTIIMVVISLFGLVDSPALILAIPVLFLSGLIFAEVSLIAVA
                     TVPGIDTFNYFYTLLMTPMFLFSGIFFPLDNLPPVVAKIAFFTPLYHLVNFCRSFSSG
                     KIWAGMGDLAWIVVVAMILAPYPFRLMRRRIIK"
     gene            complement(974..1711)
                     /locus_tag="VMU21_10705"
     CDS             complement(974..1711)
                     /locus_tag="VMU21_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO78031.1"
                     /translation="MPINIVTASGLTKDYGPLRAVEGVDFTITKGECFGFLGPNGAGK
                     TTVMRIIYCFMPPTSGEVKVFGMNVVESPSRIKARIGVMPQDSNLDPDLSVFENLIVY
                     SRYFDIPKKKSAPLAWELLDFVEMREKAHTKIESLSGGMKHRLLLARSLVNNPELLVL
                     DEPTTGLDPHSRHAVWDKLKDLMARDTTLLLTTHYMEEAEKLCDRVAIMDQGKIVTID
                     SPSRLMDLHGGNLEHVYLKLTGRHLET"
     gene            complement(1725..2618)
                     /locus_tag="VMU21_10710"
     CDS             complement(1725..2618)
                     /locus_tag="VMU21_10710"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HUO78032.1"
                     /translation="MVGIEGFIKNASLLDSLTDPWNRLIMVMGGSDTGKTTLVESLVN
                     FLSEQGEVGIVDLDMGQSHIGPPTTIAWGKTARGFQDWERIRVEEFYFTGSLTPMGNL
                     LPSVAGAKLMTERALSFCERIVVDTTGLIAEPAGRVLKQYKIDALCPDIILALERSGE
                     LGHILDSFRFHSRPKVQRLKMPDQVTTKSMVKRSNYRLGKMEHYFRHACTREVSLEEV
                     GIRFTRVPSGLGRMDLKNRVVSFRDKDNRDIALGIIREIELKDNLLSVLTPLGSENKI
                     SAIVIGSTEIDLTNSVMKDRR"
     gene            2814..>4245
                     /locus_tag="VMU21_10715"
     CDS             2814..>4245
                     /locus_tag="VMU21_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78033.1"
                     /translation="MEKKEEKEEKKREKLPIDAKLLTDAVIELNISRRSVSLYPREHP
                     FTRESLEKAFNLLQKLFEIRSSITIGIAKDTLVVDEYTLERKNPVFREFALSIHAKGI
                     AAITFYSGATMDELLEFHQLITAKDVPVGKAFIELAEKKGLTHIKLSPLDISQFSFIE
                     GSFREKASDAKIWEDYIYGLLEGRLADSDAEGVVLGLPPEAIADFINRKMDEDVAEVT
                     YDRVITTYLKRRDDSAIRSEAFSKFISMVQSLSAEIKQQFLKRAFSSSPTDVREAEQL
                     ISELTQEEIERITRLFSEHSSVIPESLRNIVDKLTSAKRERGFFDMLKEGKALVDDVE
                     LDENIVRLFSDDQFKSFVAEGYQAMLERMLKGITLQDSPMLEEVRRECTEEIVDSAAS
                     ELMLELVDLDTTSRDDFLNLLPRLFDLVNEFFETGRFEEISNVYNSLYTPALNGKFGA
                     EASGMVESFFSSKETVSKMMDSLKVWG"
BASE COUNT         1121 a         1024 c         1044 g         1056 t
ORIGIN      
        1 cagtgcgatt acatacagca gcccaagaac cggtgccatt accaacttcc cgtatacatg
       61 ctccctatgt ccttcctgtg ctgtcatttc cttcacccct ttctttcctt attttccctg
      121 ttgattccag actaccatac tgaaaagaca aatataattg gaagaaactc ctagttcaaa
      181 gggaaatgta tcctaatgtg cgatgcaaaa gctggtatga agttctttct attttatgat
      241 ccgtcttctc atgagcctga aggggtaagg ggcaaggatc attgccacca caacaatcca
      301 cgctaaatcc cccatgcctg cccatatctt tcctgatgaa aatgatctgc agaaattcac
      361 caggtggtag aggggtgtga agaaagcgat ctttgccacg accggaggca ggttgtcaag
      421 ggggaagaag atgccggaaa atagaaacat gggcgtcatc agaagggtat aaaaataatt
      481 gaaggtgtcg atccccggaa cagttgcaac tgctataagg gatacctctg caaagatcag
      541 tccgctcaga aaaaggaccg gaatggcgag tatgagggca ggggagtcca cgagaccgaa
      601 gagggagatg acgaccataa ttatggtgcc gtagagcatg ctctttgttg ccccccacat
      661 cagttcccct gccacgagat cattgaggtt cacaggcgta gcgaggatag cgtcaaaggt
      721 cttctggaag gtcattctca cataggtgcc gtaggtgcat tcgtagattg ccgcgaacat
      781 ggaagacgag gcgattatgc caggagcgat aaagttcata taaggcacgc cgttaatatc
      841 tttgacaaac gcacccagcc cgaaacccag agcgagaagg tagagcacag gctcaacaaa
      901 gtttagggct atgctcgatt tgtagagctt agtatacacc gtaaaatttc tctgccagac
      961 cctgaaggcc ctcttaagtt tcaaggtgcc tcccggtcag tttcagatag acatgttcga
     1021 ggttcccgcc atgcaagtcc ataagcctcg aaggggagtc gatggtcaca attttcccct
     1081 ggtccatgat cgccaccctg tcgcagagct tttccgcctc ttccatgtag tgggtggtga
     1141 gaagaagggt cgtatccctt gccatgaggt ctttgagttt gtcccatacc gcatgcctgc
     1201 tgtgaggatc aaggccggta gtaggctcgt ccaggacaag cagttccgga ttgttcacga
     1261 gagagcgggc gaggagaagc ctgtgcttca tgcccccgga gaggctctct atctttgtat
     1321 gggccttttc cctcatctcc acgaaatcta aaagttccca cgccaaggga gccgacttct
     1381 ttttggggat gtcgaagtag cgggagtaga caatcagatt ttcgaataca ctgaggtcag
     1441 gatcaaggtt gctatcctgg ggcataacgc cgattctcgc tttaatccgg ctcgggcttt
     1501 caacgacgtt catgccgaat accttcacct ctccggacgt gggcggcatg aaacagtaga
     1561 tgatgcgcat gacggtggtt tttccagcgc cattgggacc caggaacccg aaacactccc
     1621 cttttgtaat ggtgaaatct acgccctcaa ccgccctgag agggccgtag tcttttgtga
     1681 gaccgcttgc agtaactatg ttgatgggca tggttgtgcg cgcctcagcg tctgtctttc
     1741 atcactgaat tcgttaagtc aatctcagtg cttccgatca caatcgcgga tatcttattc
     1801 tcactgccca agggcgtaag aacagagagg agattgtcct ttaattctat ttcccttatg
     1861 attcccagcg caatatccct gttgtcttta tccctgaaag agacaaccct gttcttgagg
     1921 tccatgcgac caagacctga cggcaccctt gtaaaccgta tccccacctc ttccagagaa
     1981 acctcccgtg tgcaggcatg gcgaaaatag tgctccatct tcccaaggcg ataattgctc
     2041 ctttttacca tcgactttgt agttacctgg tccggcatct ttaatctttg gaccttcggc
     2101 cgtgaatgaa acctgaagct atccaggata tggcccagct ctccggatcg ttccagcgca
     2161 agaatgatat ccgggcaaag cgcatcgatc ttatactgct tcaagacacg tcccgcaggc
     2221 tccgcgataa gccctgtggt atccactaca atcctttcgc agaaggagag cgccctttct
     2281 gtcatcaact ttgcccctgc caccgagggt aacaggtttc ccatgggagt aaggctgccg
     2341 gtaaagtaaa attcttcaac cctgatcctt tcccagtcct gaaatccccg cgctgttttt
     2401 ccccatgcaa tggttgtcgg aggtccaata tgggactgac ccatatctag gtccacaatg
     2461 cccacttctc cttgttcaga aagaaagtta acaagggact cgaccagggt cgtcttgcct
     2521 gtatcagatc ctcccatgac cataatgagc ctgttccaag gatccgtgag tgagtcgaga
     2581 agagaggcat tttttatgaa gccttctatc cctaccatag gtttattttt tcattttcag
     2641 tttatccccg tacaagtggc atgtcaatga gttgttcgcg ccgcaatgct aaattccacg
     2701 gcctctttta cataagaact ttctacttca agagatatac tggtattagc tcataaaata
     2761 tacgataata agaaaccaat acgttgcgtt aagacctctg cgcagccatg gatatggaaa
     2821 agaaggaaga gaaagaagag aagaaaaggg aaaagctgcc cattgatgca aagctcctca
     2881 ctgacgcggt cattgagctg aatatatcga gaaggagtgt aagcctctat ccccgggagc
     2941 atccctttac aagagagtcg ctggagaagg cctttaattt gctccagaag ctgtttgaaa
     3001 tcaggagcag catcaccatc ggtatcgcaa aggatacgct ggtagttgac gaatacacac
     3061 tggaaaggaa aaaccctgtc ttcagggaat tcgctttaag cattcatgca aagggaatcg
     3121 ctgctatcac cttttactca ggcgctacca tggacgagtt gctggaattc caccagctca
     3181 ttactgcaaa agacgttccc gtgggaaagg cctttattga actagcggag aaaaaaggcc
     3241 tcacacacat aaagctctca cctctggata tctcccaatt cagctttatt gaagggagtt
     3301 tcagagagaa agcttctgat gcaaagatat gggaagatta catctacgga ttactggagg
     3361 gaaggcttgc agattcggat gcagagggag tagtccttgg gcttcctcct gaggccatag
     3421 cggattttat aaacaggaag atggatgagg atgtcgcgga ggtgacttat gacagggtga
     3481 tcactacata tctcaagcgg agggatgact ccgctatcag aagtgaggca ttctccaaat
     3541 tcatttctat ggtgcagagt ctcagtgcag agattaaaca gcagttcctc aaaagggcgt
     3601 tcagctcctc tcctacggat gtgcgcgagg ctgaacagtt gatcagcgag ctcactcagg
     3661 aagagatcga gaggataaca aggctcttca gcgaacactc ttcagtgatt cccgaaagcc
     3721 tgagaaatat cgttgataag ctcacctcgg caaaaagaga gaggggtttt ttcgacatgc
     3781 tgaaagaggg aaaggccctt gtggatgacg tagagcttga tgaaaacatc gtaaggctct
     3841 ttagtgacga ccagttcaag agctttgttg ccgagggcta ccaggcaatg cttgagcgga
     3901 tgctaaaggg tattaccctg caggattccc ctatgcttga agaggtaaga agagaatgta
     3961 ctgaggagat agtggacagt gcagcctcag agcttatgct ggaactcgtc gatctggaca
     4021 ccacgagcag ggatgatttt ctcaacctcc ttccaagact tttcgatctg gtgaacgaat
     4081 tttttgagac aggcagattc gaggaaatat ccaatgtcta caactccctt tataccccgg
     4141 cccttaatgg caaattcggg gctgaggcat ccgggatggt ggagtccttc ttttcctcaa
     4201 aggaaaccgt atcaaagatg atggattctc ttaaggtctg gggaa
//
LOCUS       DATKOU010000223         3992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2219123, whole genome shotgun sequence.
ACCESSION   DATKOU010000223 DATKOU010000000
VERSION     DATKOU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219123"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VMU21_10720"
     CDS             <1..356
                     /locus_tag="VMU21_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78034.1"
                     /translation="RSAHNFVPDEGQGVISSLLFPRYGSYWKLDNHMFYRQGMLFVAF
                     MQNTGENSFRKFLISIQQGEAFSMALTSAYNKNLSILWEQFLEETKRRSDKSSAQTAV
                     KPDLLLASVVARATD"
     gene            384..812
                     /locus_tag="VMU21_10725"
     CDS             384..812
                     /locus_tag="VMU21_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUO78035.1"
                     /translation="MKDTLKPGIESEFTFRIPESKTVPALYPESAEFKEMPEVFATGF
                     MVGFLEWACIKAINPHLDWPGEQTVGTHIDVSHIAATPVGFEVTARVKLIVVDGRRLV
                     FEVEAHDGVDLISQGKHERYIITKDKFDLKMKEKITKKGT"
     gene            832..1497
                     /locus_tag="VMU21_10730"
     CDS             832..1497
                     /locus_tag="VMU21_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78036.1"
                     /translation="MTSEVNSFDGVLTKTEGRLIERLSTPNKIQLFLDDLAYSSEVVY
                     RSPRRVLRQRTCQCFDGALFGAAMLRRLGHPALILNLLPNDRDDDHILALFKHRGDWG
                     AVAKSNFVGLRFREPIYRTLRELVLSYFEQYYNVAGEKTLRGYTLPLNLKTFDKFHWM
                     TRDEPLDLIAQRLDEIRRVFILTRPVLTRLSLVDKRSRQAGLLGANKAGLYQPPRRGH
                     SSR"
     gene            1893..2846
                     /locus_tag="VMU21_10735"
     CDS             1893..2846
                     /locus_tag="VMU21_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78037.1"
                     /translation="MHMASYAIHRDLLKIPHFRDSHMHFTKEGRPVSNLELLKIAHRY
                     RQYGIFEVHDMGHKSAPGLGAKKALAGEMRVRSAGWAIFRKAAYGVFLGKGASGKEEI
                     KKTIEEISEAGADFLKVVNSGIVSLRDEGAVTEGGFSREELKIIHGEALEHNLEMRCH
                     ANSEMAIGDAISAGASSIEHGFFMSKENIVAMAEKGISWTPTVYALQSLAPTLQYDER
                     RYLDAVIDRHLSSIHYASSIGCRLRIGTDSGSKGVDHGSSFFDELALFSKAGLTLAQI
                     LSSACVAAEEVERGNFLLVKKDFITTREIGAVYQRGQRIGL"
     gene            complement(2843..3409)
                     /locus_tag="VMU21_10740"
     CDS             complement(2843..3409)
                     /locus_tag="VMU21_10740"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HUO78038.1"
                     /translation="MKTSLGPRTIIYPTPVLVVGTYDRAGKPNVMTAAWGGICCSAPP
                     CVAVSLRKVTYTHGNVTERKAFTLNIPSETHIREADYFGMVSGRDVDKFSVTGLTPVK
                     AEFVDAPYIKEFPLILECALIQTIEIGLHTQFIGEVKDVKVDESVLTDKRVPDIEKIR
                     PMLFTPESRTYFGIGQLLGKAFSIGKKD"
     gene            3737..>3992
                     /locus_tag="VMU21_10745"
     CDS             3737..>3992
                     /locus_tag="VMU21_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO78039.1"
                     /translation="MKKILLAIDGSECAKKAVAYVGEQFSNIKDLKITLLHVLPYLAT
                     SLWDDGHILTEEERNERKKVVDRWMKNQQLKLEPMFTNAAA"
BASE COUNT         1047 a          934 c         1024 g          987 t
ORIGIN      
        1 tcaggagcgc tcacaacttt gtgccggatg aaggacaagg cgtaatttct tcactgttat
       61 tcccgcgata cggcagttac tggaagctcg ataatcacat gttttatcgc cagggcatgc
      121 tgttcgttgc atttatgcaa aacacaggtg aaaactcatt tcggaaattc ttaatctcca
      181 tacaacaggg agaagcattt tcaatggcat tgacgagtgc ttataataag aatctttcga
      241 tactctggga acaattcctg gaggaaacca aaaggagaag tgacaaaagc agcgcccaga
      301 cagcggttaa gcctgatttg cttttggcat cggttgttgc ccgcgccact gattgaggtc
      361 ctcacgtatg aaagggagac aatatgaaag ataccttaaa gcccggaatt gaatcagagt
      421 ttacctttcg cattcccgaa tcaaagactg tccctgcgct ctatcccgaa tcagctgaat
      481 ttaaggagat gccggaagtg tttgcaacgg ggttcatggt cggctttctt gagtgggctt
      541 gcattaaggc aataaatccg catcttgatt ggccgggcga gcagaccgtc ggcacacata
      601 ttgacgtgag tcatattgcc gcaaccccgg taggctttga agtaactgcg agagtaaaac
      661 ttattgtggt tgatggacga cggttggtat ttgaagtgga ggcacatgat ggcgttgatc
      721 tgatctcaca gggaaaacac gaacggtaca taatcactaa agacaaattt gatttgaaga
      781 tgaaagagaa aatcacaaag aaaggcacat aataaattgc tcaagaggga catgacatcg
      841 gaagtcaact catttgatgg ggtcttgacc aagactgaag ggcgactcat tgaacgcctg
      901 agcaccccta acaaaatcca gcttttcctc gatgatctgg cgtacagctc ggaagtggtg
      961 taccgtagtc cgcggcgggt tttgcgccag cggacctgcc agtgctttga tggtgccctg
     1021 ttcggtgcgg caatgctgcg ccgtctcggg catcccgcat taatcctgaa tctcctgccc
     1081 aatgaccgcg atgacgacca tatcctcgcg ttgttcaaac ataggggaga ctggggtgct
     1141 gttgccaaat cgaattttgt gggcctgcgt tttcgcgagc caatttatag aactctgcgg
     1201 gagctggtgc tgtcgtactt cgagcaatac tataacgttg caggggagaa gacccttcgt
     1261 ggctacaccc tccccctcaa ccttaagacc tttgacaaat tccactggat gacaagggat
     1321 gagccactgg atctgatcgc ccagcggctg gacgaaattc gcagggtatt tatcctgaca
     1381 aggccagtgc tcacacggct ctcactcgtt gacaagcgct cacgccaagc cggcctgctg
     1441 ggtgccaata aagccggtct gtatcagccg ccacgccgag ggcattcatc cagatgagca
     1501 gcacgttccc attgccgcct tctcaaacag gagcactgtt tccggcccct tttgtatggt
     1561 atagtaagac atgaggatta tttttgttat tcttatcgtt cttgctgcgg tctatctctt
     1621 tgcagccctt aacctgaggc tgacctatag aaagcttctc tgagggggag tccgtccctc
     1681 cgtgagagag agactcgcaa agaagatgga acagagaggc ctcacacccc ccgaatggtt
     1741 aacaaaggac gcaggaagct gcaaataagt ctcgtctagt cgtctttatg tttccccttc
     1801 ccttttttta atattacctc gatttgctca ttatcacaat ctccagggca gttcccttga
     1861 caaacacagt cggtccatta aaggatataa tcatgcatat ggcttcctat gctattcaca
     1921 gggacctcct caaaataccg catttcaggg attcccacat gcacttcaca aaggaaggca
     1981 ggcctgtctc aaatctggag cttttaaaga tcgctcatag gtacaggcag tatggcatat
     2041 ttgaggtgca tgacatgggg cataagagcg ctcccggatt gggggcaaag aaggcgcttg
     2101 cgggtgagat gagggtgcgg tccgcaggct gggccatatt cagaaaggcg gcgtatgggg
     2161 tcttcctcgg aaaaggtgcc tcgggaaagg aagaaatcaa aaaaaccata gaagagattt
     2221 cggaagcagg agcggacttc ctgaaggtag taaattcagg gattgtctcg ttgagagatg
     2281 agggagcagt cacagaagga ggattttccc gcgaggagtt gaaaatcatt catggggagg
     2341 cgctggagca taatctggag atgaggtgcc atgccaattc agagatggcc atcggtgatg
     2401 ccatatctgc cggcgcatct tccattgaac atgggttctt tatgtcgaag gaaaacattg
     2461 tcgcaatggc tgaaaaggga atctcatgga ctcccaccgt gtatgcgtta cagagcctgg
     2521 cacccacact ccagtacgat gagagaaggt acctcgacgc ggtgattgac cgccacctct
     2581 cctccattca ttatgcgtcg tccattgggt gcaggctgag aatcggcacg gacagcggct
     2641 caaagggggt agaccacggt tcttctttct ttgatgaact ggcgctattc agcaaagcag
     2701 gcctcaccct cgcgcagatt ctgtcttcag cctgtgtggc tgcagaagag gttgaaaggg
     2761 gcaattttct tctggtgaag aaggatttta ttaccacaag agaaataggg gccgtgtatc
     2821 agagagggca gcgcataggg ctttaatcct tcttccctat ggaaaatgcc ttgccgagga
     2881 gctgtcctat cccaaagtag gtgcggcttt ccggggtaaa gagcatgggc cttattttct
     2941 cgatatccgg cactctcttg tccgtgagta cagactcatc aacctttacg tccttcactt
     3001 ccccgataaa ttgggtgtgc agccctattt cgatggtctg gatcagcgca cactcaagga
     3061 taagaggaaa ctccttgatg tagggggcat ccacgaattc tgccttaaca ggagtaaggc
     3121 ctgtgaccga aaacttgtcc acatcccttc ccgaaaccat gccgaaataa tcggcttccc
     3181 tgatatgggt ctctgaaggg atattgaggg taaaggcctt tcgctctgtt acattcccat
     3241 gggtgtaggt aacttttctg agagataccg cgacacaggg aggagcggaa cagcatatcc
     3301 ccccccacgc agcggtcatg acatttggct tgcccgccct gtcataggtt cccaccacca
     3361 acacgggggt gggataaatt atagtgcgtg gacccagcga tgttttcatg aaatcctcca
     3421 aaggcaaata gcctttctta cggatatctt cattcctctc ctccttaggt gtcctgtgcc
     3481 aaatggttgg caggactgcg taatatatcc tgatttcatc ttaatagttt tatgtcgttt
     3541 tttcaatccc ttgactcttc atgaaaagga gtctcagggg attcccattt cattccataa
     3601 gtgaaatgcg cgaaaaaata ccgagccacc attagaaatt gcaaaaattt agcgtacaat
     3661 gtaattaatg gtatagtctg cactgtgaag gtagcgtagg tgatgtgcgc aaaaaacttt
     3721 aacggaggaa aaagagatga agaagatatt gctggctatt gatggttcag agtgtgcgaa
     3781 aaaggcggtg gcctatgtgg gtgagcagtt ttctaatata aaagacctaa agataaccct
     3841 gctccatgtt ctgccctatc tcgccacgtc actctgggac gacggacata tcctcactga
     3901 agaagaaaga aacgagagga agaaggtcgt ggacagatgg atgaaaaacc aacagttgaa
     3961 actcgaaccc atgttcacga atgctgccgc ga
//
LOCUS       DATKOU010000224         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_310560, whole genome shotgun sequence.
ACCESSION   DATKOU010000224 DATKOU010000000
VERSION     DATKOU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310560"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VMU21_10750"
     CDS             <1..889
                     /locus_tag="VMU21_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUO78040.1"
                     /translation="PDGLGTLSMRYLVLSLLMLLSLPLGSLAGYREEFEREFLSKPWA
                     GEQVEEDACIACHAAEKMDPKFKNIPQSWKMSIHYQHNVSCHDCHGGDPKDAEMSMSR
                     QRGFVGTPKYAEVPEFCGKCHVGILKNYLESGHGKALKSTGKGPNCVTCHGSHKEEQY
                     IKKANIDIINERLCSKCHSYERAKIMKQALFLTEKKMGELEDGFSRLKAEGVYVEEDE
                     KGLFSIQAEFRTLFHTVDVSLVKERTDEFANKLEPIETKVKNTFRELRFRRNFSAFIM
                     LLFAGMGIVVFVLSKPPRE"
     gene            1040..1345
                     /locus_tag="VMU21_10755"
     CDS             1040..1345
                     /locus_tag="VMU21_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78041.1"
                     /translation="MMRKSLALLVVLSILLVFSYGCASKDYVKQQVDPLVDRISKLEA
                     GMQSLKDCCAKADAAAAKAEAAAKAAQDAAKAAEAAAKRSQVGAEKATKSFELHQAK"
     gene            complement(1495..2721)
                     /gene="nrfD"
                     /locus_tag="VMU21_10760"
     CDS             complement(1495..2721)
                     /gene="nrfD"
                     /locus_tag="VMU21_10760"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HUO78042.1"
                     /translation="MGYLIKNFFIALRQIFIGNKKYYLWVCFLLFLILIGASAYMTQA
                     AKGLIITGMRDQVSWGFYISNFTFLVGVAAAAVLLVVPAYVYNFKPIKEIVLFGELLA
                     ITAISMCLLFVMIDMGQPLRGWHILPVIGAMNFPVSLLAWDVLVLNGYLAINTCIAFY
                     VLYRLSIGKEYKMSVIGPLIILSIPWAVSIHTVTAFLYNGLAARPFWNASILAPRFLA
                     SAFCSGPAIMLIIFQLIRKIFKDVDIDDRAIFKIAELIAYAMAINLFLLGAETFEVFY
                     SGTIHEYPMQYLYFGLHGKAKLVPYIWFATFLNITAFLLFLLPKTRENFTTLNMACIF
                     VIVGVYIEKGMGLVIPGFVPDTLGEIYEYGPTVPEVLISMGVWATGALIYTLLLKFAI
                     PIYTGKLRFETRQQKA"
     gene            complement(2731..3657)
                     /locus_tag="VMU21_10765"
     CDS             complement(2731..3657)
                     /locus_tag="VMU21_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO78043.1"
                     /translation="MKRRERSRIEIPVNRRTFLKGTALGLAATALPLNKADASFWEAF
                     FQKYYREMNEEEKKKVIQRLEKEYLQKYKKEIKVGDEKALPGVLFGYGLDLSRCIGCR
                     RCVYACVDENNQSKDPQIHWITVLRFKKGEKFTDLEESEKYYNPKEVPEPGYFYMPVQ
                     CQQCENPPCVKACPTQATWKDPDGIVVVDYNWCIGCRYCMAACPYGARKFNWASPNRK
                     PEEINPNTHYLGNRPRYKGVVEKCTFCIQRTRNGRYPACVEACPVGARKFGNLLDPNS
                     EMRYLIEHKRVFRLKEDLNTNPKFYYFFAYGR"
     gene            complement(3665..>4115)
                     /locus_tag="VMU21_10770"
     CDS             complement(3665..>4115)
                     /locus_tag="VMU21_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78044.1"
                     /translation="APPEAAEHEVPKKFFVPPPPFTPGIFPCSQCHQGMPVNRKQRKL
                     EQMHQEILLNHMPGGWCFDCHNPDNRDTLRLANGKIVSFQESYYLCAQCHGTVFRDWK
                     VGLHGKRTGMWDGEKQYLLCVHCHWPHDPVFKAIKPLPPPVRPSDIR"
BASE COUNT         1031 a          981 c         1085 g         1018 t
ORIGIN      
        1 tcctgatggc ctggggacac tatcgatgag atatctcgtc ctctcattgc ttatgctttt
       61 gagccttccc ctgggctctc tcgcaggata ccgtgaagag tttgagaggg agttcctgag
      121 taaaccctgg gcaggggaac aggtggagga agatgcctgc attgcgtgcc atgcggctga
      181 aaagatggac cccaagttta agaacatccc gcagagctgg aagatgagta tccattatca
      241 gcataatgtc tcatgccatg actgtcacgg cggtgatccc aaggatgcgg agatgtccat
      301 gtcgcgccag aggggttttg tgggtacccc gaaatatgcc gaggtccccg agttctgcgg
      361 gaaatgccat gtggggattt taaagaatta tctcgagagc ggacatggaa aggcgctgaa
      421 gtccaccggg aagggaccga attgcgtgac ctgccatggt tcccacaaag aggagcagta
      481 tataaagaag gcaaatatcg acattatcaa tgagcggctc tgttcaaagt gccattctta
      541 cgaaagggcc aagataatga aacaggccct cttcctcacc gaaaagaaaa tgggagagct
      601 ggaggatggt ttcagcaggc tgaaggctga gggggtctat gttgaagaag acgaaaaggg
      661 cctgttcagt atacaggcgg aattccgcac cctctttcat accgttgacg tatcccttgt
      721 gaaagagaga acagatgagt tcgcaaataa gctggagccc attgaaacaa aggtgaagaa
      781 tacctttcgt gagcttcggt tcagaaggaa tttctccgca tttatcatgc tgctctttgc
      841 tggtatgggc attgtggttt ttgttctctc caagcctccc agggaataaa tcttcccccg
      901 aagcgtctga actttaaacc cccaagtgtc cccggcattt tctcgtcctc atttttttga
      961 ttaaaaagta ttgcaactca gtgaatttcg gtgtaatctt ttattaggtg atgaacggtg
     1021 tctggtgaaa ggaggtgaaa tgatgaggaa atctctcgcg ctgttggtgg tgctcagcat
     1081 tttattggtt ttttcctatg gatgtgcttc aaaggattat gtgaaacagc aggttgaccc
     1141 tctcgtggac aggatcagca aactcgaagc agggatgcag agcctgaaag actgctgtgc
     1201 gaaggcggac gctgctgcgg caaaggcaga agcggcggca aaagctgctc aggatgcggc
     1261 aaaggctgca gaggctgcgg cgaaaaggtc ccaggtcggg gctgaaaagg caacaaagtc
     1321 cttcgaatta caccaggcaa agtagcagac ctatagcaga cctaattcaa agggcccatt
     1381 tctctttatg atgggccctt tctctattca attgaatctc tgctctttct gcttctgagg
     1441 atatgatctc tcatgcgtac aaggctgacc gccgtaacta caatccagtg aatgttatgc
     1501 cttttgttgt cttgtctcga atctcagctt gcctgtatag atgggtatgg caaacttaag
     1561 caatagggta tagatgagcg cgcctgtcgc ccagacgccc atggagatga ggacctcggg
     1621 cacggtcggc ccgtattcgt agatctctcc cagagtgtcc ggcacaaagc ccgggattac
     1681 gagccccatg cctttctcga tgtagacgcc gacgatgaca aagatgcagg ccatgttgag
     1741 ggtagtaaaa ttctcccgcg tcttgggaag gagaaagagg agaaaggcag tgatattcag
     1801 gaatgtcgca aaccaaatat aggggacgag ctttgctttt ccgtgaagtc cgaagtagag
     1861 gtattgcatg ggatactcat gaatagtccc tgaatagaag acctcaaagg tctctgcacc
     1921 aaggagaaac aaattaatag ccatggcata ggcgatcagt tcagcgatct tgaagatggc
     1981 acgatcatct atatcaacat ccttgaagat cttcctgatg agctgaaata taatgagcat
     2041 gatcgcagga cctgagcaga atgccgaggc aaggaatcgg ggtgcaagga tggaggcatt
     2101 ccagaatggc cttgccgcaa ggccattgta gaggaaggct gtcaccgtat ggatgctcac
     2161 tgcccagggt atggaaagta ttatcagggg accgataaca gacattttgt actctttccc
     2221 tatggagagc ctgtacagga cataaaaggc aatacaggta ttgatcgcaa ggtagccgtt
     2281 caagacgagc acgtcccagg ccaaaaggga cacaggaaag ttcatcgcgc cgatgaccgg
     2341 caaaatgtgc caccccctga gcggctgacc catatcaatc atcacgaaga ggaggcacat
     2401 ggaaatggcg gtgatggcga ggagttcgcc aaacaagaca atctctttga taggtttaaa
     2461 attgtatacg taggccggga cgacgagcaa aaccgcagca gctgcgactc ccacaaggaa
     2521 cgtgaagttg gagatataga acccccatga tacctggtcc ctcattccgg tgatgatcag
     2581 acccttcgct gcctgggtca tgtatgcgct cgcccctatt aaaatgagga aaagcaggaa
     2641 gcagacccag aggtagtatt tcttgttccc gataaaaatc tgccgcaagg ctatgaaaaa
     2701 gttttttatg agatagccca ttcgctcctc ctatcgtcca tacgcaaaga aatagtagaa
     2761 cttcggattg gtgttcagat cttccttaag cctgaagacc cgtttgtgct ctatgagata
     2821 ccgcatctcg ctgttgggat cgaggaggtt gccgaacttc cgcgcgccca cagggcacgc
     2881 ctccacgcat gccgggtacc ttccgttccg tgttctctgt atgcagaaag tgcatttctc
     2941 tacaacgcct ttataccggg ggcgattgcc gaggtagtgt gtgttcgggt ttatctcttc
     3001 cggctttctg ttcggtgacg cccagttaaa ttttcttgcg ccgtaagggc atgcagccat
     3061 gcagtacctg cagccgatac accaattgta gtccaccacg acaataccgt cagggtcctt
     3121 ccatgtggcc tgtgtcggac aggcctttac gcaaggagga ttttcacact gctgacattg
     3181 aacgggcata taaaagtatc caggctcggg tacttccttg gggttatagt atttctctga
     3241 ctcttcgagg tcagtgaatt tttcaccttt cttaaacctg aggaccgtta tccagtgtat
     3301 ctggggatct tttgactgat tgttctcgtc aacacatgca tagacgcacc ttctgcagcc
     3361 gatgcaccgg gaaaggtcaa ggccgtaacc aaaaaggacc cctggcaagg ccttttcatc
     3421 tcccaccttg atctctttct tatatttctg gaggtactcc ttttcgaggc gctggatgac
     3481 cttttttttc tcctcctcgt tcatctcccg gtaatacttc tggaaaaatg cctcccagaa
     3541 cgaggcgtct gccttattta agggaagggc tgttgcagcc agcccaaggg cggtaccctt
     3601 caagaaggtc cttcgattca cggggatctc aattctgctg cgctctcttc ttttcataga
     3661 cacctcatct gatatcggac ggccttaccg gcggaggcag tggtttaatg gctttgaata
     3721 ccgggtcatg cggccagtgg cagtggacgc aaagcaggta ctgcttttct ccgtcccaca
     3781 tgccggtcct tttcccgtga agacccactt tccagtcacg gaaaaccgtt ccatggcact
     3841 gggcgcagag gtaatatgac tcctggaaac tcacaatttt accatttgca agcctcaggg
     3901 tatccctgtt gtccggattg tggcagtcaa aacaccaccc gcccggcata tgatttaaca
     3961 ggatttcctg gtgcatttgt tcaagtttcc tctgttttct gttcaccggc attccctgat
     4021 ggcactggga gcatgggaat atgccagggg tgaagggggg agggggaaca aagaacttct
     4081 tgggaacctc atgttccgca gcttccggag gagcg
//
LOCUS       DATKOU010000225         7916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4164524, whole genome shotgun sequence.
ACCESSION   DATKOU010000225 DATKOU010000000
VERSION     DATKOU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4164524"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VMU21_10775"
     CDS             complement(<1..917)
                     /locus_tag="VMU21_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HUO78045.1"
                     /translation="MIKHDVKDITLARKGKLRIEWAAHEMPVLKSITERFRKERPLRG
                     IRVAACLHVTTETASLMETLKAGGAEIALCASNPLSTQDDAAASLVKNSGISVFAIKG
                     EDTKTYYRHIRDALSLRPQITMDDGADVVSTLHRDARHLLKGVIGGTEETTTGVIRLK
                     AMAADGALAYPIIAVNDAYTKHLFDNRYGTGQSTMDGIIRSTNRLIAGSVFVVCGYGW
                     CGRGTAMRAKGHGARVIITEIDPLRALEATMDGYELMPISDAARIGDIFVTATGDINV
                     ISKSCFPKMKDGAIVCNTGHFNVEVDIQSL"
     gene            complement(961..2112)
                     /gene="metK"
                     /locus_tag="VMU21_10780"
     CDS             complement(961..2112)
                     /gene="metK"
                     /locus_tag="VMU21_10780"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUO78046.1"
                     /translation="MARKNYYFTSESVTEGHPDKIADQISDSILDSIIGKDPMGRVAC
                     ETLVTTGLAFVAGEITTNCYVDIPSIVRETIRDIGYTRAKYGFDYETCAVITSIHEQS
                     SDIAMGVDPGGAGDQGLMFGFACNETPELMPMPIMLAHKLAMKLTEVRKKDILGYLRP
                     DGKTQVTIEYRDAVPHRIDTVVVSTQHSPDVIMKEMTEDIIEKVVKPVLPPDLFDEEH
                     IKYYINPTGRFVVGGPMGDTGLTGRKIIVDSYGGVGSHGGGCFSGKDPTKVDRSGAYM
                     SRYIAKNLVAAGIADRVEVQIAYAIGLPDPVSILVDTFGTGKVSQETMVKLIKDNFPL
                     SPKGIIDALNLRRPIYRKTAAYGHFGRNERDFTWEKTDKAKILKKEAGL"
     gene            complement(2236..2646)
                     /locus_tag="VMU21_10785"
     CDS             complement(2236..2646)
                     /locus_tag="VMU21_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HUO78047.1"
                     /translation="MIKYINVKSRMRNEFIDITDKIQEVVREEGISHGICYLFVPHTT
                     AGITINEGADTSVQRDIQTALSKLIPVEAEYYHREGNADAHIKSTLVGISKAVFIDEG
                     KLLLGTWQAIYFCEFDGPRHRRVAIKIHKTVKTS"
     gene            complement(2647..3657)
                     /gene="lpxK"
                     /locus_tag="VMU21_10790"
     CDS             complement(2647..3657)
                     /gene="lpxK"
                     /locus_tag="VMU21_10790"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HUO78048.1"
                     /translation="MGPWEFLYYLGYSYKKTLGLKRRKRLPRMVISIGNLTVGGTGKT
                     PAVIALAQEAKKRGHQPCILTRGYKGKAAGPCVISDGSGSLLGVEEAGDEALLMAEKL
                     KQIPVIKGNDRYEAGMFALNASIPKPSLFILDDGFQHWILDRDMDILLIDSTNPFGNR
                     KLLPSGPLREPLTEMKRADIVVITKARSHPTGFPGIEGDLIHEIRGYNRNAPLYNSSH
                     LPVGLKTTSGQDLPLDVISGKTVFGFCGIGNPDSFKHTLAGIHARVRGFMTFRDHHRY
                     RQRDIRRIYDAADECSANWIVTTEKDIMRLKDFPLWDNLVSLQIEFKIEESFYDKVLR
                     EA"
     gene            3772..4578
                     /locus_tag="VMU21_10795"
     CDS             3772..4578
                     /locus_tag="VMU21_10795"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF002360.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HUO78049.1"
                     /translation="MPVKGRGPADSLWSVRMRASRQVKSQKSKVRGPRLDEVHISGAE
                     GLYEFSEVPEITKEYSLRALHHPRGRPDRILITMERVIQKPKTAPLLPSSTVQCASPD
                     AARKVIQELLLTAGVSRKAIHCGIGIVTRRVAMHGAALVLGESAVRVEPDRRRGVRVS
                     RLGILREEERRLCRRLARQGINTATVKEALVLASKVASCRHVLAELCVSDDPDYTTGY
                     VASRRLGYVRVPCIKHTGSSSGGRVFFIKEGVDVKRIVEYLERTPVIVGS"
     gene            4668..5552
                     /locus_tag="VMU21_10800"
     CDS             4668..5552
                     /locus_tag="VMU21_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUO78050.1"
                     /translation="MKSPVILIANPAAKRASEQTIQKAVTLLRSAGHEVHVRLTQERG
                     DAEGWAREAAKDGAPLVIAAGGDGTFNEVINGIANSRTPMAIVPMGTTNVLAKELGVP
                     EDVAVATEIALRGKVHPVSLGRVLCVSHSSPVSRYFCLMAGIGFDGEAVYGVRWSLKR
                     HSGKGAYIISGLKTLMKYSPGDLRFTVNGTAYAGYSAIVGNASKYGGNFKVTPDAMLR
                     DPHLRIVIMHGKRRRDILRYIYGIFRERHLLLKDITYLKGDSVRIEGMARIQIDGDYL
                     GTTPATITVVPDALQLIY"
     gene            5660..6973
                     /locus_tag="VMU21_10805"
     CDS             5660..6973
                     /locus_tag="VMU21_10805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO78051.1"
                     /translation="MACLLLIDDEETITLSLRTFFEKKGYDFINTYTGKEGLDTALKE
                     LPDIVLLDLHLPDMHGMTILREIKAVHPDMVVIIMTGFGEIKEAVEAIKLGAEHYFQK
                     PIDLDELTVVVERNLGIRQLRREVLLSKKTPYPIIGRSRQVQGLMHLISLMAENSSTT
                     VLIEGETGTGKELVARNIHVLSMRADRPFVDINCASIPEHIFESELCGYEAGAFTDAK
                     TTKKGLLEIAEGGTLFLDEIGEMPQPLQAKLLRVIETKTFRRVGGTRDIKVDVRTIAA
                     TNKDLAECVKEGSFREDLYYRLNVMPIKIPPLRERAEDIPMLAEFFLFDTAGNMGRKV
                     GSFDKDALNALCAYSWPGNIRELRNVLERAVILCQGQVITTGSLTLPVSSPEKTPEPL
                     TLSEVERIHIRNILASSGNNRTKAAKVLGISRSTLNEKIKTYQLV"
     gene            7195..7605
                     /locus_tag="VMU21_10810"
     CDS             7195..7605
                     /locus_tag="VMU21_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO78052.1"
                     /translation="MKRVLVVDDEPSILLSLSHLFSNEEVIVITSSRIEDAEEALEHY
                     SFDLVIADIRLSGIDGIEGLELLSYIRKIRPETKVILMTAYGSEVMKESALRRGAYYY
                     YEKPIDMSSLVKKAGDCGIPVVINKRRGGAGCLS"
     gene            7593..>7916
                     /locus_tag="VMU21_10815"
     CDS             7593..>7916
                     /locus_tag="VMU21_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO78053.1"
                     /translation="MSVLKKVLIVDDSKLIHQMYRLVLMRFKDCKLVDAMNGLEAMEI
                     LSRESGFDLILLDINMPVMNGIQFMEKLKETGAHRNIPVIVISTEGKEEDTIRAINLG
                     AWGYIM"
BASE COUNT         1909 a         1966 c         2113 g         1928 t
ORIGIN      
        1 agagactgga tatccacctc tacattaaaa tggcccgtat tgcagacaat tgcaccgtcc
       61 ttcatcttgg ggaaacagga ttttgaaatg acatttatat ctcctgttgc ggtcacgaag
      121 atatcgccta tccgcgccgc atccgaaatg ggcatcagct catacccgtc catggtggct
      181 tcaagggccc tcagggggtc aatctcggta atgatcaccc gcgcgccgtg accctttgcc
      241 ctcatggcag taccccttcc gcaccatccg tatccgcaga ccacgaagac agatcctgca
      301 atgagtctgt tggtggacct gatgatgcca tccatggtgc tttgtcctgt accgtaacgg
      361 ttgtcaaaga ggtgttttgt gtacgcgtca ttcactgcga tgatgggata tgcgagggcg
      421 ccgtctgctg ccatggcctt aagtctgatc acgccggtgg tggtctcttc ggttcccccg
      481 atgaccccct tcagaagatg gcgcgcgtct ctgtggagag tggagaccac atcggcaccg
      541 tcatccatgg tgatctgggg cctcagtgac agggcgtccc tgatatgcct gtagtatgtc
      601 ttggtgtctt ctccctttat ggcaaaaacc gagatgccgg aattcttcac aagggatgca
      661 gccgcgtcat cctgcgtgct caggggattt gatgcgcaga gggcgatttc ggctcctcct
      721 gccttaaggg tctccatgag gctggcggtc tctgtggtca catggaggca ggcagcgacc
      781 cttattcccc tgagaggcct ttcctttctg aaccgttcgg taatgctttt gaggaccggc
      841 atttcatggg ccgcccactc aatccgcagc ttgccttttc ttgcaagtgt tatgtccttt
      901 acatcatgct tgatcattaa tcctccaatt gtcgagctat gagctgtcag ctgtcagcta
      961 ttaaagtcct gcttcttttt tcagtatctt tgccttgtcc gttttttccc aggtaaaatc
     1021 tctctcattg cggccaaaat gaccgtaggc cgcggtcttt ctatagatag gcctccggag
     1081 attcagggcg tcgatgatcc ctttggggga caaggggaaa ttgtctttta tgagtttgac
     1141 catggtctcc tgtgatacct tgcctgtgcc gaaggtgtcc acaaggatag agacaggatc
     1201 gggcagaccg atggcatagg caatctggac ttcgacgcgg tcagcaatcc ctgctgcaac
     1261 aaggttcttg gcgatgtagc gggacatata ggcgccggag cggtccacct tggtggggtc
     1321 ctttccagaa aaacagccgc cgccgtgact gcccactccg ccatagctgt ccacaatgat
     1381 ctttctcccg gtgagccccg tatcacccat gggaccgcct accacgaaac gccctgtggg
     1441 gtttatataa tatttgatat gttcctcatc aaaaagatcg ggcggaagga cgggcttgac
     1501 caccttttca atgatgtcct ctgtcatctc cttcatgatc acatcaggac tgtgctgagt
     1561 tgaaactacc acggtgtcga tcctgtgggg gaccgcatca cggtattcta tggtcacctg
     1621 ggttttgcca tcaggcctga gatatccgag gatgtctttt ttcctcactt cggtgagttt
     1681 catggcgagc ttatgggcga gcataatggg catgggcatc aactcgggtg tttcgttgca
     1741 cgcgaatcca aacatcaacc cctggtcgcc ggcccctcca gggtcaaccc ccatggcgat
     1801 gtccgatgac tgttcgtgaa tggaggtgat taccgcgcat gtttcgtaat caaagccgta
     1861 tttggccctt gtatagccga tgtccctgat ggtctctctc acaatagagg ggatatccac
     1921 atagcagttt gtggtgatct ctcctgcgac aaacgcaagc cctgtggtga caagggtctc
     1981 acacgcaacc ctccccatag ggtccttgcc gataatggag tccaatatgg aatcagaaat
     2041 ctggtcagct attttatctg gatgaccctc tgtgacagac tcagaggtga aatagtagtt
     2101 ctttcttgcc atagtcaaac ctcccgatta tggaatcata tattttaatc gcttttctta
     2161 aaaaatgaaa gcataaaaag agcacatcga gatcgtctct tgcgcaggaa aggaaggaaa
     2221 aggcaggagg aacagctacg atgtctttac ggtcttgtga atctttatgg ccactctcct
     2281 gtgtctcgga ccatcaaatt cgcagaaata tatggcctgc catgtaccga ggagaagctt
     2341 cccttcatct atgaatacag ccttcgagat gcccacaagg gtagatttta tgtgagcgtc
     2401 ggcattccct tccctgtggt agtattctgc ctcaacggga atgagtttgc tcagggcggt
     2461 ctggatatcg cgttgcacgg aggtgtctgc tccctcattg atggtaatac cggcggtggt
     2521 atgggggacg aacaggtaac agattccgtg agatatccct tcttccctca ccacttcctg
     2581 aatcttgtcc gtgatgtcga tgaattcatt ccgcatcctg ctcttcacat tgatatattt
     2641 gatcatctaa gcctccctca gtaccttatc ataaaagctt tcttcgatct taaactcaat
     2701 ctgtaacgaa acaagattat cccatagagg aaaatctttc agtctcatta tatctttttc
     2761 cgtggtaaca atccagtttg cggagcattc gtctgcagca tcatagatgc ggcgtatatc
     2821 ccgctggcgg taacgatgat ggtccctgaa ggtcataaag cccctgaccc gtgcgtggat
     2881 tccggcgaga gtatgcttga aggagtcggg attccctatt ccgcagaaac cgaacactgt
     2941 tttgccggaa attacatcaa gtggcaggtc ttgccctgaa gtggtcttta gaccaacagg
     3001 gaggtggctg ctgttgtaga ggggcgcatt ccggttgtat ccccttatct cgtggatgag
     3061 gtcgccctct atcccgggga accctgtagg gtgcgaacgg gcttttgtga tcacgacgat
     3121 gtccgcccgc ttcatttctg tcaacggctc cctgagtggt ccggagggaa ggagttttct
     3181 gtttccaaag gggtttgtgc tgtcaatcag gaggatatcc atgtccctgt cgagaatcca
     3241 atgctgaaac ccgtcatcga gaatgaagag tgagggtttg gggatggaag cattcaacgc
     3301 gaacatgcct gcttcatacc ggtcattgcc ttttataaca gggatttgtt tcaacttctc
     3361 agccatcaga agcgcctcgt cccctgcctc ttccacaccc agaagtgaac cactcccatc
     3421 gcttattacg catggtcccg ctgcctttcc tttgtacccc cgcgtgagga tgcagggctg
     3481 atgccccctt ttcttcgcct cctgagcgag ggcaatgact gcaggggtct tccccgttcc
     3541 ccccaccgtg agattgccta tgctgatgac catgcggggg agcctctttc ttctcttaag
     3601 acccagggtt tttttgtaag aatagccgag gtagtataaa aattcccatg gacccataat
     3661 cgtccagaat cctatcaaaa gtgataggga aaagcaagcc cggacatagc gtcacgtttc
     3721 ttgactgcgc aggccacatc aatacataat gaacgcataa tgcggaagaa aatgcctgtc
     3781 aaaggtcggg gacccgcgga ttctctctgg agcgtcagga tgagggcctc caggcaggtc
     3841 aaaagtcaaa agtcaaaagt caggggtcct cgactcgacg aagtccatat ctcaggtgca
     3901 gagggactct atgagttttc agaggttcct gaaatcacaa aagaatattc cctgagggca
     3961 ctgcaccatc ccaggggaag gcctgacagg atcctcatta ccatggaaag agtaatacaa
     4021 aaaccgaaaa cggctcctct cttgccaagt tcgacggttc aatgtgcttc gcctgatgcg
     4081 gcccggaagg taatacagga attactgctc actgcaggtg tttctcggaa ggcgattcat
     4141 tgcgggatcg ggattgtgac gcgcagggta gccatgcatg gggcggcatt ggtgctgggt
     4201 gaatcagccg ttcgcgtgga gccggacaga cggagagggg tgagggtatc gcgactgggg
     4261 atactcagag aagaggaaag aaggctttgc aggaggctcg cacggcaggg catcaatact
     4321 gccacagtga aagaggcgct tgtcctcgca tcaaaggtgg cttcgtgccg ccatgtgctg
     4381 gctgaactct gtgtatcgga cgacccggac tacacgacag ggtatgtcgc gtcaagaaga
     4441 ttggggtatg tccgcgtccc ctgtataaaa catacgggga gttccagcgg cgggagggtc
     4501 ttttttataa aagagggagt agacgtaaaa aggatagtcg aatatctcga acgaacccct
     4561 gtcatagtgg ggagttaagg cagcataggg cagccgagtt cagtcacctg agccctttgt
     4621 tccctcgggc tggaaagctt ctctggtaag aggtataatg ggtttcgatg aaatctcctg
     4681 tcatccttat cgcgaaccct gcggcaaaga gggcttcgga acagacgatc caaaaggcgg
     4741 tcacgcttct ccgttccgca ggccatgaag tgcatgttcg gctcacacaa gaaaggggag
     4801 atgccgaagg gtgggcaagg gaggcggcaa aggacggcgc tcccttggtt atcgctgccg
     4861 gaggtgacgg tacctttaac gaggtgataa acggcattgc gaattcccgg acgcccatgg
     4921 ctattgtccc catgggtacc acgaatgtcc ttgcaaagga actgggagtc ccggaagacg
     4981 tggcagtcgc aaccgagatt gccctccggg ggaaggtgca tccagtctcc cttggaaggg
     5041 tattgtgtgt ctcccattcc tcaccagtca gccgttactt ttgtctcatg gccgggatcg
     5101 gctttgatgg agaggcggtc tatggtgtga gatggtccct caagagacac tcgggaaagg
     5161 gcgcgtatat catcagtggc ctgaaaacac tcatgaagta ttcccccggg gacttgcggt
     5221 tcacggtaaa tggaactgcc tatgcaggat actctgcaat cgtggggaat gcctccaaat
     5281 atggcggaaa tttcaaagtc acccctgatg ccatgctccg ggaccctcac ctccgtatcg
     5341 ttattatgca cgggaagaga cgccgtgata tcctgagata catttacgga atattcagag
     5401 aaaggcatct cctgcttaag gatatcacct atctgaaagg cgattcggta aggatagaag
     5461 ggatggcccg tattcagatt gacggggatt atctcggcac cacccccgcc accattactg
     5521 ttgtccctga cgcactgcaa ctgatttatt aacccgccgc cctgggaaat ccagcaagtc
     5581 ctttgtgtac acagcatgag cgaatcctaa tggacagaag tattgacagt ctctagtttt
     5641 atttgagata ctgaataaca tggcatgtct gttactcatc gacgacgaag aaacaataac
     5701 cctcagcctc aggacatttt ttgagaagaa ggggtatgac ttcatcaaca catataccgg
     5761 gaaagagggt ttagataccg cgttaaagga actccctgat atcgtgctgc tggacctcca
     5821 tctgcctgac atgcacggga tgaccattct gagagagatc aaggctgttc atcctgatat
     5881 ggtggtcatc attatgaccg ggttcggtga gataaaggag gcggttgagg cgataaaact
     5941 tggggcagag cactattttc agaaacccat tgacctggac gagctcactg tggtggtgga
     6001 aaggaatctt ggcatacggc agctcaggag agaagtcctg ttgtccaaga aaactcccta
     6061 tcccataatc gggaggagca gacaggtaca gggacttatg caccttatca gtctcatggc
     6121 agaaaactcg tccaccaccg tactcattga aggggaaacg ggtacgggga aggaactggt
     6181 agcgaggaac atccatgtcc tgagcatgag ggctgacaga ccttttgtgg atatcaactg
     6241 cgcctcaatt cctgaacaca tctttgagag cgagttgtgc ggttacgagg caggtgcatt
     6301 cactgatgca aagaccacca agaagggtct tcttgagatt gcggaagggg gcaccctttt
     6361 cctcgatgaa ataggggaga tgccccagcc gttgcaggca aaactcctgc gggtcatcga
     6421 gacgaagacc ttccggaggg tcggcggcac gagggacatt aaagttgatg tgaggaccat
     6481 cgccgccacc aacaaggacc tggcggagtg cgtgaaggaa ggttccttca gggaggacct
     6541 ctattaccgc ctcaatgtaa tgcccatcaa aatacctcct ctgcgggaaa gggccgagga
     6601 catccctatg ctcgcagagt ttttcctctt tgatacagca ggcaacatgg gcaggaaggt
     6661 cggctccttt gataaggatg cgttgaacgc cctttgcgcc tacagctggc ccggtaatat
     6721 aagagagctg cgaaatgtgc tggagagggc cgtgatcctt tgccagggcc aggtgattac
     6781 caccggctcc ctcacccttc ctgtgagctc tcctgaaaaa acacctgaac cacttaccct
     6841 gagcgaagtg gaacgcatcc atatcaggaa catactcgca tccagcggaa acaaccggac
     6901 aaaagcggca aaagtgctcg gaatttcccg ctccaccctc aatgagaaga taaagaccta
     6961 ccagctcgtc tgaccgatta tcgactaacc cgtcctgttt tcatacaatc ataaacccat
     7021 accatgcttt ctcttttccc gctgaatagg gaaggtaata ccagagccct ccttaactcc
     7081 tcgctaaaaa aggatatttt tctgcgtgca tctgcctggg aatagaaaga ttttccttgg
     7141 cattcatttt gcgtataagg aattcgagca aataactcgc aggaggaagc aagcgtgaaa
     7201 agagtcctgg tcgtggatga tgagccttca atcctactca gcctttccca tctcttcagc
     7261 aatgaagaag tgatcgtgat tacgagcagc aggattgagg atgctgaaga ggctttggag
     7321 cattattcct ttgatctcgt cattgcagat atcaggctga gcggaattga cggaatcgag
     7381 ggtcttgagc tcctaagcta tataagaaaa atacgacccg agaccaaggt cattctcatg
     7441 accgcctatg gttctgaggt gatgaaggag tcagccttaa gaaggggggc ctattattac
     7501 tacgaaaaac ccattgatat gagcagcctt gtgaaaaagg ccggggactg cggaatcccg
     7561 gtggtgatca acaagcggag gggaggtgcg ggatgtctgt cttaaagaag gtcttgattg
     7621 tggatgattc caagttaatc catcagatgt acaggctggt gctcatgagg ttcaaggact
     7681 gcaaactggt ggatgcgatg aacgggctcg aggccatgga gatcctttcg agggagagcg
     7741 ggtttgatct cattctcctt gatatcaaca tgccggtaat gaacgggatc cagttcatgg
     7801 aaaagctgaa ggaaacggga gctcaccgga acattccggt gatcgtcatc agcaccgagg
     7861 gaaaagagga ggacaccatc agggcaataa acctcggggc atgggggtat atcatg
//
LOCUS       DATKOU010000226         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1854136, whole genome shotgun sequence.
ACCESSION   DATKOU010000226 DATKOU010000000
VERSION     DATKOU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854136"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="purM"
                     /locus_tag="VMU21_10820"
     CDS             <1..795
                     /gene="purM"
                     /locus_tag="VMU21_10820"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HUO78054.1"
                     /translation="TVGIDLVAMSVNDILTSGAEPLFFLDYFSTGKLEPEKAVMVVDG
                     IATGCREAGCALIGGETAEMPGFYQEGEYDLAGFAVGVVDREKMINGANIGEGDTLIG
                     LASNGLHSNGYSLVRKVFFERGKIDVSTYVPQLKSTLGDELLRPTRIYVKAFNALKER
                     IWIKGAAHITGGGIPGNLPRVLPEGLGAVVREGSWPVPAIFRVIGKEGNVPVNDMKRT
                     FNMGIGYILVVPEKEAGDGVGLLKDAGFDSYVIGTVVKGKKEVRYE"
     gene            788..1627
                     /locus_tag="VMU21_10825"
     CDS             788..1627
                     /locus_tag="VMU21_10825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUO78055.1"
                     /translation="MSRLKGFVKKAFTPITIMLVPHSNSKHFSIRLPSIGIVLSVAFW
                     LIGCIYVISIAVNAVEYRMMKERLNYYSGQFVELNATIVALKNAESEFKMLFSLGTRE
                     RVLENVHTTSDSGSIDIEAMRNQIKDTVENVKDIKEYLKEQKDIYMATPKGWPVTGRI
                     TSPYGIRENPVHGGNDFHSGIDISVESGTPIRATADGVVSFAAWSGGSGNLVGLEHGL
                     GFSTFYAHNKNVVVKVGQRVKRGDVIAYSGSTGSSTGPHCHYEIWKDGRHVNPAKYIE
                     GRS"
     gene            1630..2007
                     /locus_tag="VMU21_10830"
     CDS             1630..2007
                     /locus_tag="VMU21_10830"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUO78056.1"
                     /translation="MFSKNTEKLESFVGTHSHFKGDIKSKGTLRIDGTVDGNIEADWV
                     VLGEKASLKGNVSARGVIVGGKIEGNVAAKEILEIKSKGKVTGDISAPKLSVSEGGIF
                     EGRSTMNREDSNVVELPVKEKLG"
     gene            complement(2124..2300)
                     /locus_tag="VMU21_10835"
     CDS             complement(2124..2300)
                     /locus_tag="VMU21_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78057.1"
                     /translation="MRTTRVLGISPEVYVTVLRKLGEYSEKNNEGKGGGLLDDHPSID
                     SRIEKIQRINFSDD"
     gene            2561..>3534
                     /gene="miaB"
                     /locus_tag="VMU21_10840"
     CDS             2561..>3534
                     /gene="miaB"
                     /locus_tag="VMU21_10840"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HUO78058.1"
                     /translation="MNVHDSQKISGVLTREGYSHAENAKDSDLIIFNTCSIRQKAEQK
                     FFSDLGRIKSLKRKRPDLKIAVAGCIAQQMGERIFRRAPYVDFVFGPQNIHRITDLIQ
                     RKSSGIANDDNPEIADTDLPVLREKKGRAWVSIMYGCDNFCSYCIVPYTRGKERSRPS
                     GNILEEIKGLSREGFKEVTLLGQNVNSYRSDINFAVLLKEINSISGIERIRFVTSHPR
                     DLCEELISAISILDKVCEHIHLPLQSGSERILGLMNRGYTYDEYRKKVDSLRKSIQGI
                     SMTTDIITGFPGETEDDHKMTIKALEDIEFDGIFSFKFSPRPGTKAAT"
BASE COUNT          987 a          709 c          971 g          867 t
ORIGIN      
        1 actgtgggaa tcgatcttgt ggccatgagc gtgaacgaca tattgaccag tggtgctgaa
       61 cccctctttt tcctcgacta tttctccaca ggaaaactgg aacctgaaaa ggctgttatg
      121 gttgtcgacg gaattgccac cggctgcaga gaggccggat gtgcgctcat tggcggggag
      181 accgcggaaa tgcccggatt ctatcaggaa ggggaatatg acctcgcagg gttcgcagtc
      241 ggggttgtgg acagagaaaa gatgatcaac ggtgcgaata taggggaggg tgatacgttg
      301 attggcctcg cgtctaacgg actccacagt aatggctatt ccctggtgag aaaggttttt
      361 tttgagcgcg ggaagataga tgtctccaca tatgtcccgc agttgaagag cacacttggg
      421 gatgagctgc tcaggccgac acggatctat gtaaaggcat tcaatgcatt gaaggaacgc
      481 atatggatca agggggctgc ccatatcact ggtggaggaa ttccgggcaa tctcccgagg
      541 gtattgcctg aagggctggg ggccgtagtg agagaaggtt cgtggccggt acctgccata
      601 ttcagggtaa ttgggaaaga gggaaatgtc cctgtgaatg atatgaaacg gaccttcaac
      661 atgggaatcg gctacatcct cgtggttccc gaaaaggagg cgggggatgg agttgggtta
      721 ttaaaggatg cgggatttga ttcatacgta ataggcactg tagtgaaggg taaaaaggag
      781 gtaaggtatg agtagattga aggggttcgt aaagaaggcg ttcactccca tcacgattat
      841 gttggtaccc cacagcaatt ctaaacattt cagcatcaga ttgccctcca tagggattgt
      901 tctttcggtt gccttttggc tcattgggtg catttacgtt atttccattg cggtcaatgc
      961 cgttgaatat cggatgatga aggagaggct caactattac tcaggacaat ttgttgagct
     1021 taatgccacg atcgttgccc tgaaaaatgc agaatctgaa ttcaagatgt tgttttctct
     1081 cggaacaaga gaaagggtgc tggaaaatgt ccataccacc agtgatagcg gttcaattga
     1141 tatagaggca atgagaaacc agatcaagga taccgtggag aatgtgaagg atatcaagga
     1201 atatctcaag gagcaaaaag atatttacat ggcaacaccc aagggatggc cggtaacagg
     1261 aagaatcacc tctccttacg ggatcagaga gaaccccgta catgggggta atgattttca
     1321 ttcaggcatc gacatctctg tagagtcagg tacccccatc agggcgacgg cggacggggt
     1381 tgtgagtttt gcggcctgga gcgggggaag cggaaatctc gtgggcctgg agcatggcct
     1441 tggcttctcc acgttttatg cccataataa gaatgttgtg gtaaaggttg gacagcgcgt
     1501 aaagagaggc gatgtcattg cgtattcagg gtccacggga agcagcacag gtccccactg
     1561 ccattacgaa atatggaaag acggaagaca tgtgaaccct gcgaagtaca ttgaagggag
     1621 gtcataagta tgttctcaaa aaataccgaa aagcttgaat catttgttgg aacccatagc
     1681 catttcaagg gagatataaa atcaaaagga accctcagga tagacggcac ggttgatggc
     1741 aatattgagg cggattgggt agttctgggg gaaaaggcaa gcctgaaggg gaacgtatca
     1801 gcgaggggcg ttattgtggg gggcaagata gagggaaatg ttgctgccaa agagatactc
     1861 gaaattaagt caaagggaaa ggtgacgggg gatatcagtg cccccaaact cagtgtgtct
     1921 gaagggggca tttttgaagg tcgctccacc atgaacaggg aggactcaaa cgtggtggaa
     1981 cttccagtga aggagaagct gggttagagc caggaagttg ctccggcggc caaagatcac
     2041 cgttcatttc agggctgatt caaagaagtt tttcccgcac ggtgtcgcag ggagacaata
     2101 tcatggtgcc cggcccatcg gccctagtca tcggagaaat tgattctctg tattttctct
     2161 atccttgaat ctattgatgg gtgatcgtcc agcaatccac cgccttttcc ctcgttgttt
     2221 ttttctgaat attctccgag ttttcttaaa acggttacgt atacttctgg agatatcccc
     2281 aggacccttg tggttctcaa cgcttcgcta tgagcctctg tttccagctc ctttgattac
     2341 gcctttttcg ccagaaaaat aaagcggcgg tgaagtgcga ttggcaattt ccttactgag
     2401 caataggcaa ggtgacaata ggtcaagtca tgggcgaagg gatcataaaa tacgcatgta
     2461 tatgtcgcgc agattgtcat cacttgcttc aaatgaagaa ttggtggtat ttttatagat
     2521 gtcccaaaaa aaattccata ttgtaacatt tggctgtcag atgaacgtac atgactccca
     2581 aaagatatcg ggtgttctga ccagggaggg gtatagccat gccgagaacg caaaggattc
     2641 agaccttatt atttttaata cctgcagtat aaggcagaag gctgaacaaa agttcttcag
     2701 cgacctggga aggataaaat cccttaagag gaaaagacct gatctcaaga tcgctgttgc
     2761 gggctgtatt gcgcagcaaa tgggagagcg aatcttcaga agggcgccct atgtggattt
     2821 tgtattcggt ccccagaaca tccacaggat caccgatctt attcaaagga aatcttctgg
     2881 tatcgcgaat gacgataatc ccgaaatcgc ggatacggac ctccccgtgc tgagagagaa
     2941 aaaggggagg gcgtgggtct ccatcatgta tggatgtgat aatttctgca gttattgtat
     3001 tgttccctat acaaggggaa aggagagaag cagaccttcc gggaacatcc tggaagagat
     3061 aaaggggctc tcccgtgaag gctttaagga ggtgacgctt cttgggcaga atgtgaactc
     3121 atacaggagc gatataaact ttgcggtatt acttaaagag attaactcca tctccggcat
     3181 agaaaggata cgatttgtta catcccaccc cagagacctt tgcgaagagt tgatctcagc
     3241 catttccatt cttgataagg tatgtgagca tattcacctt cccctgcagt ctggttcgga
     3301 gagaatcctg gggcttatga acaggggata tacatacgac gaatatcgca aaaaggtaga
     3361 ctcccttaga aagagcattc aaggaatttc catgaccaca gatatcatca ccgggtttcc
     3421 gggagaaaca gaagatgacc ataaaatgac cattaaggcg ttggaagata ttgaatttga
     3481 tggcatattt tcctttaagt tctcaccaag accgggaaca aaggctgcta caat
//
LOCUS       DATKOU010000227        10386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2229727, whole genome shotgun sequence.
ACCESSION   DATKOU010000227 DATKOU010000000
VERSION     DATKOU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229727"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /gene="ftsH"
                     /locus_tag="VMU21_10845"
     CDS             <1..1562
                     /gene="ftsH"
                     /locus_tag="VMU21_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUO78059.1"
                     /translation="LPQLREKNVIITIESAEQGYFWQILVGILPWVLIIGVWILIMKR
                     NQQIQGGPGGLFTFGASKAKLYDTKKPSITFKDVAGMDNVKMELRETIEFLKDPSRFA
                     KLGAKVPKGVLLIGPPGTGKTLLARATAGEAGVPFYSISASEFIEMFVGVGASRVRDM
                     FKKAKDSHPSIIFIDEIDSVGRTRGTGLGGGHDEREQTLNQLLSEMDGFDPHEEVIVM
                     AATNRPDVLDPALLRPGRFDRHIVIDRPAWKERKAILEIHARGKVLADDVNMETLARG
                     TPGMTGADLENLTNEAALVATRKNKEKIDMHDFEEAEDIILMGAVREETISDTEKRIT
                     AYHEAGHTLVAWELPGADPIYKVSIIPRGMAMGVTQLLPQEDRHYYPRTYLMNKLSVA
                     LAGRVAEKIVFNDSSSGAQNDLKEATSLAEKMVAQWGMSDKVGPINLGRGEEHPFLGR
                     ELSLPKRYSEEMAWVMDQEIQKMIIDAESRATEILAGKRHTLDALAEALIKEEMLEKA
                     DVERIIQGSQE"
     gene            complement(1785..2240)
                     /locus_tag="VMU21_10850"
     CDS             complement(1785..2240)
                     /locus_tag="VMU21_10850"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUO78060.1"
                     /translation="MEKELSKQELDHLIVDLLKEQSLCVIATCSDNVPRASTVEYFSI
                     ATSIYILTEGGVKINNVIHNPNVSIAIHAPFSGWQTVRGIQITGIAEIGRKGSIVYEE
                     GLDAFNKRRGLTGTVPDIMSIIKVKPSKIEYLDTALGNKGFKVRHTLVF"
     gene            2596..3306
                     /locus_tag="VMU21_10855"
     CDS             2596..3306
                     /locus_tag="VMU21_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO78061.1"
                     /translation="MLEIKSLAYKAGGKNILEGLSLTVDPAEVHAVLGTNGTGKSTLA
                     YLIMGCEGYRPSSGELVFDGKVINDLKIHERANLGITMAWQEPVRFEGIAVRDYLTLK
                     SKDIDAALHLSMVGLHPELYLHRNLDKSLSGGERKRIELASVLALHPKLAILDEPDSG
                     IDMLSTQDIVNVISAFKEHGSSVLLITHREEIVLIADRASQICNGRIVCSGNPERVAE
                     YYKSRKCFVCDGEACVYV"
     gene            3299..4234
                     /locus_tag="VMU21_10860"
     CDS             3299..4234
                     /locus_tag="VMU21_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HUO78062.1"
                     /translation="MSEFDALLKAFGEAGGDKGALAAEDTAHLVASGHKLLSMKSVEG
                     LEVNAHETLSGISAKVTVKEGIKIANPVHLCFGVLHKKGTQKIKMDVTLEKNSSAHFI
                     AHCIFPKAEKVRHIMDAVVKIGEDAEMRYSETHYHGPYGGTEVVPKSVISIDKNGRYF
                     ADFTLIDGRVGTLDIDYAVEAGENAVTELTARVFGHASDEIKIKESVVLAGENARGLI
                     KTRVALEDESSAEVTGITEGNAAGARGHVDCMEIVKDKAIAKAIPIVNVTNPLAKVTH
                     EAAIGSVDKRQIETLMAHGLTPEEAVDVIVKGILK"
     gene            complement(4419..4994)
                     /locus_tag="VMU21_10865"
     CDS             complement(4419..4994)
                     /locus_tag="VMU21_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HUO78063.1"
                     /translation="MNVVTEAIKKRRSVRSYQSKPIPRDIVRAIIDAGNEAPSAMNSQ
                     PWRFVVIEGAEVKKKLLMAALPNARKIIEMIKESDPVRYEAITKRLSELPDPVYYSAP
                     VIVFVIGSGRYANHSCPLACQNMMLAAHSIGLGSCWVGFGSMVTDDDEVKKILDLKDD
                     ETIFGPILLGYPEGYPERPPKKEPSIKWVEK"
     gene            5239..5982
                     /locus_tag="VMU21_10870"
     CDS             5239..5982
                     /locus_tag="VMU21_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO78064.1"
                     /translation="MKVREIKAKSILSKSQVYDYGLNPYVGCQHGCVYCYAKFMKRFT
                     GHRERWGEFVDVKTNAPELLSREVKKKRIGRVWISGVCDPYQPLEKKYMLTKRCLAIL
                     VENGWPFTVQTKSPLILRDIGIVNRSKDAEVGFTITTADEKIRKLFEPGAPPSMRRIE
                     ALAKLHSEGVRTFAMVAPILPGAEGLVNALKGNVEYVILDRLNYHYADWAYKKYEMHW
                     AMEDSFFSQKGEELKMAFEREGIPCQIVF"
     gene            6626..6916
                     /locus_tag="VMU21_10875"
     CDS             6626..6916
                     /locus_tag="VMU21_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78065.1"
                     /translation="MEAYKGFTGFDISGVSENVAKFIRYSLDTTFENVAKVQEFNEKI
                     VRDTIEAGKKAQVEAEKMVNESIEAGKKGWSEYRKAVEDGFKKVEEMIQPAK"
     gene            7182..7604
                     /locus_tag="VMU21_10880"
     CDS             7182..7604
                     /locus_tag="VMU21_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78066.1"
                     /translation="MNDTKGQQKRCVYCGRYFKPDRRAEERQKACKDRACQAKRKKDS
                     QRRWLESNPGYFAGRYDCLKQWRKEHPDYQRRWRAKRREIQDEIPPSKPIRTIRLVIP
                     AEWFKGEIQDEIRLKRQCGCGFFVAGGGMRDTRSDCRI"
     gene            7657..9087
                     /gene="istA"
                     /locus_tag="VMU21_10885"
     CDS             7657..9087
                     /gene="istA"
                     /locus_tag="VMU21_10885"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HUO78067.1"
                     /translation="MAEDVRQRIQYLRDVMHLSFYQIEDMTGVPRKRASKIYQGDNSG
                     NKASRPCKLKRYRSLIGDWFADYPALKAKQVYGMLKDRGMEVSYSLVVKYTRSFRQKK
                     EKVYHALTFLPGEESQVDWCFINHPSLGKLSCFVLILSHSRYLFAHVFPRASFEFFIE
                     GHLMAFSSMKGTTRSLRFDNLRTVVLRRRPDVEFNPHFLGFCRHYGIEIRLCNPGAGN
                     EKGRVERVIRTMKEMYFNKLQNYSSFEALNSGLHEWVDHKNRTVHRATEQRPIDLLKE
                     ERLKALPEIPWNNVVIHPPVKTTKTGMMIFDTNSYSVPDYLVGKSLSIHSTPDMVRIY
                     DSDKQVASHPRSFQRYKQFLNPLHRSYCRISQKAKMQRIHEVIKNMHPAMSDFLLRNQ
                     TCGEDPCKTAHEIFRLMKGHSRGMLISIASECLKRKSPRLKTFLSYLETGPSETVEIV
                     SPQNGGLLNITYKPRSLEVYDDETES"
     gene            9065..9766
                     /locus_tag="VMU21_10890"
     CDS             9065..9766
                     /locus_tag="VMU21_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78068.1"
                     /translation="MMTKPNLESLLVELGCRTPTKEVPTDRHDIIAEFLKTEMEYRRQ
                     HKIKRLLRASGIKQVKTLSQFDWHFNPKISKEDIMSFVNSQWIESSANLVLIGDTGLG
                     KSHIDSALCYEAILQGHPTTFITAFDLTSKIQKALNPAAKIDYYARIKVLCIDELGYT
                     YHKKEDTDILFQVISKRSEMLPTLVSTNLPPKDWGSIFTGAAASAILDRLSFNGRFLT
                     FEGRSYRLSRKQKLK"
BASE COUNT         2938 a         2285 c         2703 g         2460 t
ORIGIN      
        1 tcctgcccca actgagggaa aaaaatgtga ttatcaccat cgagtccgct gaacagggat
       61 atttctggca gattctggtc gggatactgc catgggtgct gatcatcggt gtctggatcc
      121 tgatcatgaa gaggaatcag cagatccagg gtggcccggg agggctcttt accttcggag
      181 caagcaaggc caaactgtat gatactaaaa aaccgagtat aacgtttaaa gatgttgccg
      241 ggatggacaa tgtaaagatg gagttgaggg agacgataga gtttctgaaa gacccctccc
      301 ggttcgcgaa actcggggca aaagtcccca agggggtgct tctgatcggg cctccgggaa
      361 cgggtaagac cctccttgcg cgcgcaaccg caggggaggc aggggttcct ttttacagca
      421 tcagcgcttc tgagttcata gagatgtttg tgggggtcgg cgcctcgcgc gtcagagaca
      481 tgttcaaaaa ggcaaaggat tcccatccca gcattatttt tatcgatgag atcgattcgg
      541 taggccgcac acgagggacg gggcttggag gcgggcatga tgaaagggaa cagacactga
      601 accagctttt gagtgaaatg gacgggttcg atccccatga agaagtgatt gttatggctg
      661 ccaccaacag gccggatgtg ctcgatcccg cgctcttaag gccgggaagg ttcgaccgac
      721 acattgtgat agacaggccg gcctggaaag aacgaaaagc aatccttgaa atccatgcca
      781 ggggtaaggt gctggcagat gatgtcaaca tggagactct ggcgaggggc acccctggca
      841 tgacaggcgc cgaccttgag aatcttacaa acgaggccgc actcgttgct acgagaaaga
      901 acaaagagaa aatagacatg cacgactttg aggaggctga ggacattatc cttatgggag
      961 cggtgagaga agagaccata agcgatacgg aaaagcggat aaccgcgtat catgaggcag
     1021 gacataccct tgtcgcttgg gaacttcccg gtgctgaccc gatttacaag gtgagcatta
     1081 tcccgcgcgg aatggcaatg ggtgttactc agctcctgcc ccaggaagac agacattatt
     1141 atccgcgtac ctatcttatg aacaaattga gtgtagccct cgcgggaagg gttgcggaaa
     1201 agatcgtttt taacgacagc agcagcggcg cacagaacga cctgaaagag gcaacttcat
     1261 tggctgagaa gatggtggca cagtggggaa tgagcgacaa ggtcggcccg ataaatctcg
     1321 gacgtggaga agaacacccg ttcctgggca gggagttatc tctcccgaag cgctacagcg
     1381 aggagatggc ctgggtcatg gaccaggaaa tccagaagat gattatcgat gcggagtcaa
     1441 gggcgactga aatattggcc gggaagagac atacacttga tgcccttgca gaggccctga
     1501 ttaaggagga gatgcttgaa aaggcagatg tggagcggat aatacagggt tcgcaagaat
     1561 aagaagtcat ccatatcagc ggcgtacgca acctttgcaa tctcgtcatg aatattcttt
     1621 ttctgtgaca tttagttcct cctcttttat aaaaaagtat atttccaggt gatacacttt
     1681 tggggtagcg ctttcaagtg tggataacag gaagggaata ggttcaccgg ttcagattca
     1741 ctgcgaagaa ggtcttggag gtaacacggt catgaaaaat gacctcagaa tacaagagta
     1801 tgtctcactt taaagccttt attccctaac gcagtatcta gatattcaat tttactcggc
     1861 ttaactttta tgatactcat gatatctggt actgtgccgg ttagtcctct gcgtttgttg
     1921 aaggcatcaa gaccttcttc atatacaatt gaacccttcc tccctatctc ggcaatcccg
     1981 gttatctgaa tacctctcac ggtttgccaa ccactaaatg gtgcgtggat agctatggag
     2041 acattggggt tgtgaataac attatttatc ttgacccctc cttcggtaag gatgtagata
     2101 gatgtagcta ttgaaaagta ctctacagtt gatgccctcg gtacattatc tgaacaggtg
     2161 gcaatcacgc acagagattg ttcctttaac aaatcaacta tgagatgatc gagttcttgt
     2221 ttgctaagct ctttttccat attcccgggc cgcatgacgt aacatcatct ccaaagtgaa
     2281 tggattagca gtggagcatt ctctcaccgc aattaaacgg gcacgcggta acacataagg
     2341 caattattct cacccaagac tgttgctttc ccgctgtact cagcacaatt atatcatgct
     2401 tggtacgtgt ggtgtttagg aggaaggacg tgagagctat gatttcttgc ttgtcaatcg
     2461 gcctcactga acgtattgta agggcaaaag gaacaaagaa cttttctctc tattcaaaaa
     2521 gagtcagcgg tgcgatactg gtagtaggaa tatattttgg tgtaacgtat ataacgtata
     2581 ttgagagata tgccaatgct tgaaataaag agtcttgcgt acaaggccgg gggtaaaaac
     2641 atccttgaag gtctgtcatt aacagttgac cccgccgaag ttcacgccgt cttaggcacg
     2701 aacggcaccg gaaaaagcac cctcgcatac ttaatcatgg gctgtgaggg ctaccggcct
     2761 tcatccgggg aactggtgtt tgacgggaag gtaatcaacg accttaagat ccatgagcgg
     2821 gcaaacctgg gaataaccat ggcctggcag gagcctgtga gatttgaggg tattgcagtc
     2881 agagattatc tgacgctcaa aagcaaggac attgatgcgg ccctgcacct cagcatggtc
     2941 ggtcttcatc ctgagctcta tttgcatcgc aatcttgaca agtccctgag cggcggtgag
     3001 agaaagagga ttgagcttgc gtccgtcctc gcactgcatc cgaagctcgc cattctcgat
     3061 gagcccgatt ccgggatcga catgctttct actcaggata tagtcaatgt cattagtgcg
     3121 ttcaaggaac atggttcatc tgtacttttg attacgcacc gggaggagat tgtgttaatt
     3181 gctgacagag catctcaaat atgtaatggc aggatcgtat gctcagggaa tcctgagaga
     3241 gttgccgaat actacaagtc gaggaaatgc tttgtctgcg atggggaggc gtgcgtgtat
     3301 gtctgagttt gatgcattat tgaaggcatt tggagaggca ggaggggata aaggcgctct
     3361 ggctgcagag gatacggccc acctcgtcgc aagtggtcat aaattgctga gcatgaaaag
     3421 cgttgaaggc cttgaggtta atgcacatga aaccctgagt ggcattagcg ctaaggttac
     3481 ggttaaagaa ggtataaaga tagcgaatcc ggtccacctc tgtttcggcg ttctgcataa
     3541 aaaggggacc cagaagatca agatggatgt taccctagaa aagaactcgt cagcccattt
     3601 catagcacac tgcatctttc caaaggcgga aaaagtgcgg catatcatgg acgctgtggt
     3661 taagatcggc gaggacgcag agatgagata ttcggagacc cactatcacg ggccttacgg
     3721 aggcacagaa gtagtcccga aatcagttat cagcatagac aagaatggga gatacttcgc
     3781 agattttacc ctgattgacg gcagggtcgg tactctcgat atcgattacg cggttgaggc
     3841 aggagaaaat gcagtgaccg aattgacggc acgggtcttc ggccatgcca gtgatgagat
     3901 taaaattaag gaaagcgtgg ttctcgcggg cgaaaatgcc cggggactga tcaagacaag
     3961 ggtagcactt gaggatgagt cgtcggctga agtgacaggc atcactgagg gaaatgcagc
     4021 cggcgcaagg ggccatgtcg actgcatgga aatcgtcaag gataaagcca tcgcaaaggc
     4081 tataccgata gtcaatgtca caaatcctct cgcaaaagtc acccacgaag ctgctatcgg
     4141 aagtgtagac aaacgccaga tcgagaccct tatggcacac gggctgacgc ctgaagaggc
     4201 ggtagatgtg atcgtgaaag gcatactgaa atgaacactt caatttatcg gatttgcatc
     4261 gtttagtatc agaacggatg tcagcaggga tgccggtatc tgttcttctt aacttatgag
     4321 aaagtattta ctccatgaag gaatttgcac ggaacacaac aaatgtggtg tccagcctgg
     4381 gcaatggtat ttactgcgca attgcgggag atcctcagtt atttctctac ccacttaatt
     4441 gagggttctt ttttgggagg cctctccgga tatccttcgg gatatccaag aagtataggg
     4501 ccgaatatcg tctcgtcatc cttaaggtca aggattttct tcacttcatc gtcatcggtc
     4561 accatggagc cgaaaccgac ccaacaactc ccaagtccaa ttgaatgtgc tgcgagcatc
     4621 atgttctggc acgccagagg gcaagagtgg ttggcgtatc gccctgatcc tatcacgaaa
     4681 acgatcaccg gggctgagta ataaacaggg tccggcagct cgctcagacg tttcgtaatc
     4741 gcctcgtacc ttactgggtc tgactctttt atcatctcga tgatcttcct ggcgtttggc
     4801 agggcggcca tgaggagttt ctttttcact tcagcgcctt cgatcaccac gaatctccac
     4861 ggctggctgt tcattgccga tggggcttca ttgcctgcat cgatgatcgc tctgacgata
     4921 tctctgggga tcggcttaga ctgataggac ctgaccgatc ttctcttctt tatagcctcg
     4981 gtaacgacgt tcataactcc ctccttggtg ttcaaatagg cttaaacaca ttgccaagat
     5041 tgtttattca cattttacca ttaataaccc agcgggcaaa gaagtggatg cttcaatccg
     5101 gggtctgctg gcaacataac ctgggacaat gtctattaca cgccgatagt tgcagcgaag
     5161 ccgaaccatt gcgaattgcc aggctggcat tttgcgcaat tatagcaaag acctccgggc
     5221 tataataaag tctgaaatat gaaggtcaga gaaataaagg caaagagcat tctgtctaaa
     5281 tctcaggtct atgattatgg cctaaatcct tatgttggct gccagcatgg ttgcgtctac
     5341 tgctatgcga aattcatgaa gaggtttaca ggtcaccggg agagatgggg agagtttgtt
     5401 gatgtaaaaa caaatgcccc tgaacttctg tcccgtgagg taaagaaaaa aagaatcggg
     5461 agggtctgga taagcggtgt gtgcgatccc tatcagcctc tggaaaagaa atatatgctc
     5521 acgaagagat gtcttgcaat cctcgtggaa aacggctggc cttttacagt ccaaaccaaa
     5581 tctcctctta tcctgaggga catcggaata gtgaacagat caaaagatgc cgaggtcgga
     5641 ttcacgataa caacagcgga tgagaagata cgaaagctct ttgaacccgg ggcgccacca
     5701 tcaatgagac gaattgaggc cctggcgaaa cttcactcgg aaggggttag aacatttgcc
     5761 atggtggccc cgatccttcc aggcgccgaa gggctggtga acgcgcttaa aggtaatgtg
     5821 gagtatgtga tccttgacag gctgaactat cattacgcag actgggctta caagaaatat
     5881 gaaatgcatt gggccatgga agacagcttt ttttcccaaa agggagagga gttgaagatg
     5941 gcatttgaaa gggaaggtat tccctgtcag attgtatttt agaattgctg acaaaacgca
     6001 cagagagtga acacaggcaa tgattgcttc atccgaaggc tacgtaagtt acattcccat
     6061 ccacgtctgt catctcatta acgaaaggat atttatccgc agacaataaa gttcttactt
     6121 tctggggaag agcggagggg tgagattcta actcacggtt ctcattgttt ttaaagggtt
     6181 tttggcctcc tccgaaacga atccgaaacg agtctctaaa ccctatctgc ctccacttag
     6241 gggattcaat ctattttgac ctgcccatgc aacctatgac cataataaac ctacaatggt
     6301 gattattatt tgataaatca aatagttatc aatggttcag gtcatgtctg aagaaggagt
     6361 tattttcccc ttgacaaatt acgaaagtaa tgctatagta ttcctaaaga atgacataaa
     6421 gaatatctgg atggtagact ttaggcgctt agagcctatc aaaggaagga gatgcagatg
     6481 aaacagacgc gagagaaaaa aaccgtcgta acccctcaag acgaagggag cgatgaggaa
     6541 taagcataga gcagcaggcg tcgggaaggt gagagtttct caccaagaac aatcaaacat
     6601 cataacaaaa caaggaggac agaaaatgga agcatacaaa ggtttcacag gttttgacat
     6661 cagtggtgta agcgagaacg ttgcgaagtt tatcaggtat tcacttgata cgacattcga
     6721 gaacgtggcg aaggttcagg agttcaatga aaagatagtg agagacacga tcgaagcggg
     6781 caagaaggcc caggtcgagg ccgagaagat ggtgaacgag tccatcgaag ccgggaaaaa
     6841 gggctggagt gaatacagaa aggctgtaga agacggcttc aagaaagttg aagagatgat
     6901 acagcccgcg aagtaagctg caattcagaa ttgtgcaagg ggggatccct tgcacaattc
     6961 tgtcttattt gaccttagac tttctggagc aacaagacca acaaagcaag gaggtaaatg
     7021 tgatgattac gaatcattca gtagatgtag ggggtaattg gaagtgcccc aatcaggata
     7081 acctacctat tcccaattga gttgttattc cagtaatacc gcagtagtct gagcttgcca
     7141 tttattcctc gtagccggcc gcagttttca taagataatg gatgaacgat accaaggggc
     7201 agcaaaagag gtgtgtgtat tgtggccgct atttcaaacc agaccgccgg gcagaggaaa
     7261 gacagaaagc atgcaaggac agagcttgtc aggcaaaacg gaagaaggac agtcaacgga
     7321 ggtggctgga atccaatccc ggttatttcg cgggaagata cgactgccta aagcagtggc
     7381 ggaaggaaca tccggactat cagcgccgat ggcgggcaaa aaggcgtgag atacaagacg
     7441 agataccccc ttcaaagcca ataaggacaa tacgtttggt gataccggcg gaatggttca
     7501 agggtgagat acaagatgag ataaggctga agaggcagtg tggatgcggg tttttcgttg
     7561 ccggaggggg gatgcgagat acaagatcag attgcaggat ctgaagggcc tatttaccat
     7621 agagacagca taagattggc ctggaggaaa acgacaatgg ctgaagacgt aaggcagagg
     7681 atacagtatc ttcgcgatgt gatgcatctg agcttttatc agattgagga tatgacaggg
     7741 gtccccagaa aacgggcatc gaagatatat cagggggaca actccggcaa taaggcaagc
     7801 aggccctgca agctgaagag atatcgctcg ttgataggcg attggttcgc cgattatccg
     7861 gccctgaagg ctaagcaggt ttatggaatg ctgaaagaca gggggatgga agtcagctat
     7921 agccttgtgg tgaagtacac gagatctttc aggcagaaga aggagaaagt ataccatgcc
     7981 ctaacctttc tgcccggaga agagtcccag gttgactggt gctttatcaa ccacccttct
     8041 ttggggaagc tctcctgctt tgttcttatc ctgagccact cgcggtatct atttgcccat
     8101 gtattccccc gcgcctcctt tgagtttttc atagaaggtc atctcatggc cttttcctca
     8161 atgaagggta ctacccgcag tctgaggttc gataacctcc gtaccgtcgt tttaagacgc
     8221 agaccggacg ttgagtttaa tccgcatttc ctgggattct gccgtcatta cggtatcgag
     8281 atacgcctgt gcaatcccgg agccggcaac gagaagggcc gggtcgaaag ggtcattcgg
     8341 acgatgaaag agatgtattt caataagctg caaaactact catcgtttga ggccctcaac
     8401 agcggtctgc acgagtgggt tgaccataag aaccggaccg tccatagggc cactgagcaa
     8461 agacccatcg atctgcttaa ggaagaaagg ctgaaggccc tgcctgagat accgtggaac
     8521 aatgttgtca tccatcctcc agtgaaaaca acaaagaccg gcatgatgat ctttgatacg
     8581 aactcctatt ctgtgccgga ttacctggtc gggaagtccc tgtccataca ctcaaccccg
     8641 gatatggtca gaatatacga ttcggataaa caggtggcct ctcatccaag gtcgtttcag
     8701 cggtacaaac agttcctcaa tcccctgcac cgcagttatt gcaggatatc tcaaaaggcc
     8761 aagatgcagc gcatccatga ggtgataaaa aacatgcacc cggccatgtc cgactttttg
     8821 ctgaggaacc agacctgcgg ggaagacccc tgcaaaacgg cgcatgagat cttcaggctc
     8881 atgaaaggac atagccgtgg catgcttata agcatcgctt cagaatgtct caaaaggaaa
     8941 agcccgaggc tgaagacatt tctctcctat ctggaaactg ggccgtcaga gactgtcgag
     9001 atcgtttctc ctcagaatgg aggtcttctg aacattacgt ataaaccgag aagcctggag
     9061 gtgtatgatg acgaaaccga atcttgaatc cctgcttgtg gaactcggct gcagaacacc
     9121 gacaaaagag gttcccaccg accgccacga tataatcgca gagtttctca aaacggaaat
     9181 ggagtatcga aggcagcata agatcaaaag gctccttaga gcctccggta taaaacaggt
     9241 caaaaccctg agccagttcg actggcactt caacccgaaa ataagcaaag aggatattat
     9301 gtccttcgta aactcccagt ggatcgaaag ctccgctaat ctcgtcctga tcggcgatac
     9361 cggtctgggc aaatcgcata tcgactctgc cctctgctac gaagcgattc tccaaggtca
     9421 tccgactact ttcataacgg ccttcgatct cacttcgaaa atccagaagg ctctaaatcc
     9481 cgccgccaaa atcgattatt acgccaggat caaggtgcta tgtatcgatg aactcgggta
     9541 tacgtatcat aaaaaggagg atacagatat cctcttccaa gtcatctcca aaagatcgga
     9601 aatgctgccc actcttgtat ccactaactt gccgccgaag gactggggct ccatctttac
     9661 cggagccgct gcatcagcca ttcttgatcg gctcagcttt aacgggcggt tccttacttt
     9721 cgaagggagg tcttatcgat tatccagaaa acagaaactc aaataaatgc acttaagata
     9781 gctcgtacag ctctgcgtcc attcgccttt tgtatctcag gtgcttggag aagtgcgtct
     9841 caaccccgga tccccttgcc ccaatattat cctttttggg acagcccaaa ttatccccta
     9901 cagaagaagt tgccctagaa ctgtatgtga gaagtggaaa cgtcgagggg catgatcttg
     9961 acaactggct cgaagcagaa agcattgttt gggacgatat cgagcaagag atgcagtgca
    10021 cgctcgaatt gcggacatag aattcaactc accccattat agtttcccta gtccaaactc
    10081 ccctaactga cagctttaaa caagagctta atacccaaaa taagagatgc gtaaatacaa
    10141 aagggcgata ttaggatttt tatgccgtga tttcgctcag aattcaacca gaacgcttag
    10201 ccgaaaggtt gcgtatatct gcatttagtt atatgatgta ttcaagaaag gtgcagaacc
    10261 gtaggttttg caagtgggag taagtgtaag aaagaaaggg gtgtagatta tgtttagcag
    10321 aaaaatcata aataagaaaa tggtgaatgt tatgacaggc atggtcgtga tggttgttgg
    10381 ggcgct
//
LOCUS       DATKOU010000228         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3385349, whole genome shotgun sequence.
ACCESSION   DATKOU010000228 DATKOU010000000
VERSION     DATKOU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3385349"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1309
                     /locus_tag="VMU21_10895"
     CDS             311..1309
                     /locus_tag="VMU21_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUO78069.1"
                     /translation="MRKAAESVHRKVVVCSLIVVMFFIAVNCVYSAQYANPFLLADVT
                     MVEDTKGRTDWVMIDCRDEKAYAAGHIPGAISLGGACGKVLRDPTFRAKKTEDLEKML
                     GNAGVSADKHVILYADAKLITSASVAFWILEYLGHDKVHFLNGGIEAWEEAGKPLDKA
                     EKKLPPATFKANVMKERIATSEEMAEIGKGELKGENVIDSRTENEHKGADIRALRGGY
                     IPNTTLNVSHVKTYDVDTGRIHTMEELETIFDKLDRGKRTIAYCQTGTRSTLIYLELR
                     LMGFKDPSNYDDSWIIYGSNLNYPVANENWYDFVRVNDTIKAVDELKKAVEELKKR"
     gene            1528..2685
                     /locus_tag="VMU21_10900"
     CDS             1528..2685
                     /locus_tag="VMU21_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUO78070.1"
                     /translation="MDYFLTEEQIMIRDLARQIAEEKVVPVREELDEKEEFPWEIMKV
                     MAQADLFGLFIPEEYGGLGKGCLELCIAVEELSRACLGVSTTYAANALGTFPLLLHGS
                     DEQRKKYLPDIAAGKKLVAFGLTEANAGSDAAGIQTTARRERNEYVLNGTKQWITNGG
                     EAEIYTIIAMTDKDKGPRGASAFVVEKGTPGFHFGKKERKMGIRASVTRELIFDNCRV
                     PKENIIGKEGLGFIVAMKTLDSSRTGVGSQGVGVAQGAFDEAVKFARQRVQFGHPIIS
                     FQAVQHMLADMATEIEAARSLVYSVARYIDSGAKDVSKESAIAKVFATDVGMRVTTNA
                     VQVMGGSGYMREYPVEKMMRDAKILQIYEGTNQIQRNVIGQALIREAAKKR"
BASE COUNT          762 a          602 c          820 g          640 t
ORIGIN      
        1 agaactttcc ccttgaaaaa taactcacac tgcatttcag gaggcacctc agtgtgcctc
       61 ctgaaaaccc atggcacgca tagtatagcc actggtgtca gcttctcctg gcaaaagtcc
      121 ttttaaccgg caaacagctt atacgttatt tcctccctgt agtttctgtg ttgcatttat
      181 ttgaaatctg attatacttt tattaaacaa atgacgcgaa aggggtagga aatacaatag
      241 ggatttggtg tgaacgaagc attctttttt aatcatcata taatcaatcc gaagtaagga
      301 ggtcattatc atgagaaagg cggcagagag cgtgcacagg aaggtggtgg tatgttcact
      361 tatcgtcgtg atgtttttca ttgcggtcaa ttgtgtatac agcgcccagt acgcaaatcc
      421 tttccttttg gcagacgtaa caatggttga ggacaccaag ggaaggactg actgggtgat
      481 gattgattgc cgcgatgaaa aggcctatgc ggcaggccac attcccggag caataagcct
      541 gggaggggcg tgcggcaagg tactcaggga ccccacattt agggcaaaga agacagaaga
      601 cctggagaaa atgttgggga atgcaggagt gagcgcggac aaacatgtga tcctctatgc
      661 cgatgcgaag ctcattacca gtgcttcggt tgcgttctgg attctggaat accttggtca
      721 tgacaaggtg catttcctta atggcggaat agaggcatgg gaggaggcgg gaaaacccct
      781 tgataaggct gaaaagaaac ttcccccggc cacctttaag gcgaacgtga tgaaagagag
      841 gattgcgaca agcgaggaaa tggcagagat aggaaagggt gagctgaagg gtgagaacgt
      901 gatcgattcc aggacagaga atgagcataa gggcgcggat ataagggcgc taagaggggg
      961 atacatccct aacaccactc tcaatgtctc ccatgtaaag acctatgatg tggataccgg
     1021 cagaatacac accatggaag agctggaaac aatctttgac aaacttgaca ggggcaagcg
     1081 taccatagcc tactgccaga caggaacacg ctctacactc atctacctgg agttgcggtt
     1141 gatggggttt aaagaccctt ccaactacga tgactcatgg attatctacg gcagtaatct
     1201 gaattaccct gttgccaatg agaactggta tgactttgtg cgggtgaacg ataccataaa
     1261 ggcggtggac gagctgaaga aagctgtgga ggagttgaag aagcgataac caccgtctgc
     1321 tttccaaacc tctctctttt ctgttacaat gtgcatgcag aatatgcgga gcaagtgcca
     1381 ggcgtttgag caaagggtgt tcttgtgatc ccggctgccc tgtcctgaaa cggatagcga
     1441 gcgaagagaa taaagaagaa ttcaatgcgt gaacgtaccc tgctgtcttg cggcagtgtt
     1501 cttcattact gaagcggagg gttgataatg gactatttct taacagagga acagataatg
     1561 ataagggatc tcgcacgcca gatcgcggag gagaaggtgg tgccggtgag ggaagaactg
     1621 gacgaaaagg aggaattccc gtgggagatc atgaaggtca tggcccaggc tgatctcttc
     1681 ggcctcttta tccctgagga gtacggaggt cttggaaagg gatgcctcga gctttgcatc
     1741 gcggtggaag aactctccag ggcatgtctg ggggtgtcca cgacctatgc tgccaatgcc
     1801 ttgggaacat ttcccctcct cctccacggc tctgatgagc agaggaagaa ataccttccg
     1861 gatatcgctg ccgggaaaaa acttgttgca ttcggtctca ccgaggcgaa tgccggaagt
     1921 gatgccgcag gaatacagac gaccgcaagg cgtgagagga acgagtatgt gctcaacggg
     1981 acaaagcagt ggataaccaa tgggggggag gccgagattt ataccattat cgccatgacc
     2041 gataaagaca agggcccgag gggagcctct gcgtttgttg tcgaaaaagg caccccgggt
     2101 ttccactttg gcaagaaaga gaggaaaatg ggaataaggg catcggtcac gagggagctc
     2161 atttttgata actgccgggt ccccaaggaa aatatcatag ggaaggaagg cctcgggttt
     2221 attgtggcca tgaagaccct ggacagctcc aggaccggcg tcgggtcgca gggcgtgggg
     2281 gttgcgcagg gcgcctttga tgaggcggtg aagttcgcgc ggcagcgtgt tcagttcggc
     2341 catcccataa tcagttttca ggctgtgcag catatgctcg cagacatggc aacagagatt
     2401 gaggctgcga ggtcgcttgt gtattcggtt gcccggtata tagacagcgg cgcaaaggat
     2461 gtttcaaaag agtccgccat tgcaaaggtg tttgccaccg atgtgggaat gcgcgtcacc
     2521 accaatgcgg tgcaggtgat gggtggctcg ggatacatga gggagtaccc ggtggaaaaa
     2581 atgatgcggg acgcaaagat cctgcagatt tatgaaggca caaaccagat acagaggaat
     2641 gttataggtc aggcattgat aagagaggct gcaaaaaaga ggtaacctcc cttgttaatg
     2701 tgcggagtga atgaaaggtt cctctggttt attgtgtggc aggatccttc ttcttctccc
     2761 cggtcaagtg ttcaacaaag tcttcagcgg tcttttctgc ggcaaacgcc atttcttcct
     2821 taag
//
LOCUS       DATKOU010000229         6760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2230349, whole genome shotgun sequence.
ACCESSION   DATKOU010000229 DATKOU010000000
VERSION     DATKOU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2230349"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..503)
                     /locus_tag="VMU21_10905"
     CDS             complement(144..503)
                     /locus_tag="VMU21_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78071.1"
                     /translation="MARVGEIGDYRRIEDFIALAMEGGKVDLEIELRKQLVTQKVPRG
                     VTEDMKDEMDMYLLTADYIFKVGKDMKLVSKVYVFGSSEEPADVIKVNSSIANARLKM
                     DYQRLKDAKITFEEKYF"
     gene            complement(1004..2689)
                     /gene="ettA"
                     /locus_tag="VMU21_10910"
     CDS             complement(1004..2689)
                     /gene="ettA"
                     /locus_tag="VMU21_10910"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HUO78072.1"
                     /translation="MSNEPNKVIYSMIRVSRYYEKKPVLKDISLSYFYGAKIGVIGLN
                     GSGKSSLLRILAGRDREFNGETVLSPGHTVGFLEQEPLLDDKKTVREIVEEGVQKTVN
                     ALKEYHLINEKFAEPMSDDEMAKLIERQGKVQERLEALDAWDLDSRLEMAMDALRCPP
                     GDTVLKVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAETVAWLEHHLQGYPGTII
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKKNRLQQEEKAESERQKTLQR
                     ELDWIRMSPKGRHAKAKARINSYEALLSQDMEKRAKELEIYIPPGPRLGSVVIDADNV
                     SKAYGDTILMEGMTFSLPPGGIVGVIGPNGAGKTTLFRMITGQEKPDSGTFKTGETVR
                     LSYVDQSRDDLDPNKSIWEVISGGDDVIQLGKKQVNSRAYVARFNFSGTDQQKKVSML
                     SGGERNRVHLARMLKEGANVLLLDEPTNDLDVNTMRALEEALENFAGCAVVISHDRWF
                     LDRIATHILAFEGDSRVVWFDGNYSEYESDRKTRLGAAADQPHRIKYRHLSRG"
     gene            2927..3526
                     /locus_tag="VMU21_10915"
     CDS             2927..3526
                     /locus_tag="VMU21_10915"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78073.1"
                     /translation="MSAEGEQETVKDKVAVIDIDNTLWQFCDAFYEELRKINNSFPTP
                     DKWTAFDIWEGYCSEADFFSAIHRIHLQQDSAAYQPYPEAGDFLSTLKEKGYYLIIAS
                     HRSPDARDQTERWLARHGLVYDELHLSSDKTTLFTCTDVVVDDSPYALEKALESGALG
                     TGLLFPWNRPWADNGFRLFQDLNGVLQYILSVSRPPFYR"
     gene            3634..3960
                     /locus_tag="VMU21_10920"
     CDS             3634..3960
                     /locus_tag="VMU21_10920"
                     /inference="COORDINATES: protein motif:HMM:NF018563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HUO78074.1"
                     /translation="MTKMNEFKGVTVQKKANVYFDGKVTSRTILFGDGSKKTLGVMLP
                     GEYEFSTSDREVMEILSGELEVLLPGSAGWKRIEGGTSFEVPANSKFGLRVKTLTDYC
                     CSFLNP"
     gene            3983..5503
                     /locus_tag="VMU21_10925"
     CDS             3983..5503
                     /locus_tag="VMU21_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HUO78075.1"
                     /translation="MILLIHPPVAKPSEPPAGIARLSGMLGSFGVEHRLLDANREGLL
                     HLLRSPLPTENARDTWTRRALRNLQPNLRSIRDPVLYHHPDRYKRAVRDIGRVLARVS
                     PPDKVVSLANYEDQGLSPLKSRDLLTAAEHPELNAFYPYFCSRLEGLFTSQKPMMVGI
                     SLNYLSQALTCFSMIGFIRRQFPGVKVILGGGLVTSWVKNPGWKDPFSGLVDHIVAGP
                     GEYPLLSLLGLHASERKPPRPDYSSLSQEDYLSPGFILPYSASTGCYWSRCAFCPEKA
                     EGNPYLSVPVQQVVEDLRALGQATGPSLIHILDNAISPKLLGSLSENPVGVPWYGFAR
                     VSPRLADPDFCVALKRSGCVMLKLGIESGDQHVLDALQKGISLETASAVLKNLRRVGI
                     GTYVYFIFGTPAESERSAEMTLEFTVQHSGCIDFLNLAIFNMPICEVHSPDVETRRFS
                     EGDLSLYTDFVHPHGWDRRRVRLFLKNKFRRHPAVAGILNNEPPAFTSNHAPFFAK"
     gene            complement(5620..>6760)
                     /locus_tag="VMU21_10930"
     CDS             complement(5620..>6760)
                     /locus_tag="VMU21_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78076.1"
                     /translation="VFSVLLICALCIFPVLEGTSFAVTPKIAAGWDHTVVVKPDGSLW
                     AWGWNQTGQLGDGSPIDRLTPFDSFTPFQIGIDSTWISVATGYDHTVALKPDGSMWAW
                     GLNNFGQLGDGTTTNRLTPVQIGTDTTWVSIAAGYYHTIALKADGSMWAWGLNNLGQL
                     GDGTTTDRLTPVRVGTDNRWRAIATSDVRSLALKSDGTLWVWGCFSDVKLRDSTRTGS
                     LIPVQIGTDTNWVSIAMGYSHNVALKADGSLWAWGLNNLGQMGDGTTTDRLTPVRIGA
                     DTDWVSIAAGGHHSVALKTDGSMWAWGSNYFSELGDGTTTDRLTPVRIGTDITWVSVA
                     TNWHHTAALKSDGTVWTWGWNAHAQLGDDSKTLRQTPVQIQFPQQ"
BASE COUNT         1574 a         1845 c         1576 g         1765 t
ORIGIN      
        1 tacggatttc agtaagattt cagagcaaca gattgctcag atagaatcat tgatcaataa
       61 tcgtcccaga aaatgtatgg gctttaaaac accacttgaa gtcgcttctt cttgtgttgc
      121 acttcgagat tgaatgtggg actttaaaaa tacttttcct cgaatgtaat ctttgcgtcc
      181 ttcaaccgct ggtaatccat cttcaaacgt gcatttgcga tgcttgagtt taccttgatg
      241 acatccgcag gttcctctga agatccaaac acataaacct tggaaacaag tttcatatct
      301 tttccaactt tgaagatata atccgccgta aggaggtaca tatccatttc gtctttcata
      361 tcctcggtaa cccctcgagg aactttctgt gtgacaagct gttttcttaa ttcaatttct
      421 aaatcaactt tccccccttc catagcaagg gcaataaaat cttctatcct tctataatca
      481 cctatttcac caactcttgc catatcaaac tcctcacttt atgtatttaa aatattaaag
      541 gataaaatcc catagactga gcctgctttc cattgttttt tggaaaaact ttcgacggaa
      601 ttgttaccaa ctgatgctat cagaatatgt gattcttgtc aatttaataa atcttaagac
      661 aataaaagtt tcttacggca ggcattttgc gcaaacaaga gatacacaaa aacagcctct
      721 cccatccttt tccccaccct tatttttatt ttcattgtag tactgttaca acttttagat
      781 tatctgcact atctgatagg cgtattgtcc tcatctctga cttattttct agtcctacct
      841 acgcggagtt ctccttgaaa tatttaatgg tttcaccttc tgataactga ggggtcggtg
      901 tacaccacaa catgacacat tgtctctcag gacgaaaacg cgtcccctat ttccgcccac
      961 agacggcttc tgtaatcagc agaaaaagca cccctttggg tttttatccc ctggacaaat
     1021 ggcggtactt gatgcggtgg ggctggtccg cagccgctcc cagtcgtgtc tttctgtcag
     1081 actcgtactc tgaatagttc ccgtcaaacc agaccaccct gctgtcccct tcaaaggcaa
     1141 ggatatgggt tgcgatacgg tcaaggaacc accggtcgtg gctgatgacc acagcgcagc
     1201 cagcaaaatt ctccaatgcc tcttcgaggg cgcgcatggt attcacatcc agatcattgg
     1261 taggttcatc aaggagaagg acattggccc cttccttgag catacgggcc aggtgaaccc
     1321 ggtttctttc acctccggag agcatcgaaa cctttttctg ctgatctgtt cctgaaaaat
     1381 tgaagcgtgc cacataggcc cgtgagttga cctgtttctt gcccaactgg attacatcgt
     1441 ctccgcccga gatcacctcc cagatgcttt tgttcggatc gaggtcatca cggctctggt
     1501 ctacatatga aagtcttacg gtctctcctg ttttgaaggt ccctgaatca ggtttttcct
     1561 ggcccgtgat catccggaaa agggttgtct ttcctgcccc gttgggacca atgaccccca
     1621 cgatccctcc cggcggaagg gagaaggtca tcccctccat gagtatggta tctccatacg
     1681 ccttgctcac attgtcagca tcaataacca cgctgcccag acgggggcca ggcgggatat
     1741 agatttcgag ctcttttgcc cttttttcca tgtcctgact caggagcgcc tcataggaat
     1801 tgatacgcgc cttggctttc gcatggcgcc ccttgggaga catgcgtatc cagtcgagct
     1861 cacgctggag ggtcttctgc cgttcgctct cggccttttc ctcctgctgc agacggttct
     1921 tcttctgctc cagccacgaa gaatagtttc ccttccatgg aattccctct cccctgtcca
     1981 gttccagaat ccaccctgcc acattatcga ggaaatagcg gtcatgggtc acagcgatga
     2041 tagtaccggg atacccctgc agatggtgct ccagccaggc cacagtctct gcatcaaggt
     2101 ggttggtggg ttcatccagg agcaggatgt ccggtttctg gagaaggagt ctgcacaggg
     2161 ccacccggcg tctttctcct ccagagagga ccttcagcac agtatctccc ggaggacagc
     2221 gcagcgcgtc catagccatc tcgagacgtg aatccaggtc ccatgcatcc agggcttcca
     2281 gcctctcctg caccttcccc tgccgttcaa tgagctttgc catctcgtca tctgacatgg
     2341 gttcggcgaa tttctcattg ataagatgat attccttaag ggcattcact gttttctgta
     2401 ccccctcttc cacaatctcc cgcacggtct ttttatcatc cagtaacggc tcctgctcaa
     2461 ggaagcctac ggtatggcct ggggacagga ccgtctctcc attgaactcc ctgtcccttc
     2521 ctgcaaggat gcggaggagg gagcttttcc ctgatccgtt gagaccgatc acgcctattt
     2581 ttgccccata gaaataggaa agggagatat ccttcagcac cggtttcttc tcataatacc
     2641 tgctcacccg gatcatggag taaatgactt tgttgggttc attgctcacg gcagttcaat
     2701 cctttctccg cataggttca tgcacctgtc accactaaaa acatgtgcgg gctaaaagta
     2761 taccataata aagtcaatgc ctctcatgtg tcctggttct tcatatcgct ttcgggagtg
     2821 gcccgtagta tcctatacgt tactccagcg ttcgcactgt ggggctttcg gcgcttcact
     2881 ccctccacag tttatctcgg aatggtgtaa tataaagcta gagtaattgt cagctgaagg
     2941 ggagcaggaa acggttaaag acaaggtagc ggttatcgac attgacaata ccctctggca
     3001 gttttgcgat gccttttatg aggaactgag gaagataaac aacagttttc ccactcctga
     3061 taagtggacc gcattcgata tatgggaggg atattgttct gaagcggatt tttttagtgc
     3121 catccaccgg atacatctcc agcaagatag cgccgcatat cagccctacc ccgaggccgg
     3181 ggattttctc tccacgctaa aggaaaaggg gtattacctc atcatcgcga gccataggtc
     3241 acctgatgca agagaccaga cagaacgatg gctcgcacgc cacggtcttg tgtatgacga
     3301 actccacctt tcttctgaca agaccacatt gttcacgtgc actgatgtgg tggtggacga
     3361 ttcaccatac gcccttgaaa aggcgctgga gagcggtgcg ttgggaacag ggctcctctt
     3421 cccctggaac aggccatggg cagacaatgg cttcaggctt tttcaggacc tcaatggtgt
     3481 cctgcagtac atcctctcgg tgtctaggcc accattttac cgataaagcc ttccagtagt
     3541 attcactctc cacaggcttc ccttttctgc acttctgcac cccgtaagtg gtagaataga
     3601 tacgtatcac cgtgagggga gggataagac gagatgacga agatgaatga attcaaaggc
     3661 gtaaccgtgc agaagaaggc gaatgtctat tttgacggga aagtgacaag caggaccatt
     3721 ctctttggcg atggatcaaa aaaaaccctt ggtgtaatgc tccctggaga atatgagttc
     3781 tccaccagcg acagggaggt tatggagatc ctctcaggtg aactggaggt gcttcttccc
     3841 ggcagcgcag gctggaaaag gatagaggga gggacgtcct ttgaggttcc ggcaaattca
     3901 aagttcgggc tcagagtgaa gacgcttacg gactattgct gttcatttct taacccttga
     3961 atcacctttc acataaagtc cattgatact ccttatccac cccccagttg ctaagccgtc
     4021 cgaaccgccc gcaggcatcg cgaggctctc ggggatgctg ggcagctttg gggttgaaca
     4081 ccggctcctc gatgcgaaca gagaggggct gctccatctc ctccgaagcc ctcttcccac
     4141 tgaaaatgcc cgggatacct ggacaaggag ggccctccgg aatcttcagc ctaaccttcg
     4201 ttccataaga gaccctgtcc tctaccacca cccggaccgt tacaagaggg cggtgagaga
     4261 cataggaagg gtactggccc gtgtctctcc gccagacaag gtggtcagtc tggcgaatta
     4321 cgaagatcag ggactctctc ccttgaagag cagagatctt ctcaccgcag ccgaacatcc
     4381 tgaactgaat gccttttatc cttatttctg ctctagactt gagggtctct tcacgtccca
     4441 aaaacccatg atggtgggta tttccttgaa ctatctgagc caggcattga cctgcttttc
     4501 catgatcggt tttataagac gacaattccc cggagtgaag gtgatcctgg ggggtgggct
     4561 tgtcacctca tgggtgaaga atccgggatg gaaagatccc ttctcaggcc tggtggacca
     4621 tatcgtggct gggccgggcg aatatccgct cctctcgctc cttggcctcc atgcatcaga
     4681 aaggaagccg ccaaggcccg attactcctc tctttcgcag gaggattacc tttcaccagg
     4741 ctttatcctt ccttacagcg cctccacagg gtgttactgg agcaggtgcg cattctgccc
     4801 ggagaaggca gagggaaacc cttatctctc tgttccggtg cagcaggtcg ttgaggattt
     4861 aagggccctc gggcaagcca cagggccctc cctcatccat atcctggaca atgccataag
     4921 tcccaagctc ctcggttctc tttccgaaaa ccctgtgggc gtcccctggt atggatttgc
     4981 ccgtgtcagc cctcgcctcg ctgacccgga tttctgtgtg gcgctgaagc ggtcgggatg
     5041 tgtgatgctc aaactcggta ttgaatcagg agaccagcat gtgctggacg ccctgcagaa
     5101 agggatcagt cttgagaccg catccgctgt tctgaagaat ctcaggagag tagggatcgg
     5161 aacctacgtg tatttcatct tcggcacccc ggcagaaagt gagaggtcag cggaaatgac
     5221 ccttgagttc actgttcagc acagtggctg tatcgatttc ctcaacctcg cgattttcaa
     5281 catgcctata tgcgaagtcc attccccgga tgtcgaaaca cgaagattct ctgagggaga
     5341 tctttccctc tacaccgact ttgtccatcc tcacggctgg gacagaagac gtgtcaggct
     5401 tttcctcaag aataaattcc ggagacaccc ggcagtggca gggatactga acaatgagcc
     5461 gcccgcgttc acctcaaacc acgcgccctt ttttgcgaag tgatatttgc gcactcattc
     5521 agcaggatgt ctcatgaatc tttgtgtttt ttgccaggac ggaaggtatg cgctacagaa
     5581 gagaattttc acatcatccc tttccgctta tccggagtgt cactgctggg ggaattgtat
     5641 ttgaacagga gtctgtctga gggtctttga atcatccccc aattgggcat gagcgttcca
     5701 tccccaggtc cacacagtac catcggactt cagggctgcg gtatgatgcc agtttgtggc
     5761 aacggagacc cacgtgatat cagttccaat ccggacaggg gtcaatctat ctgttgtagt
     5821 gccatcaccc agttcgctga aatagttaga cccccacgcc cacatagacc cgtccgtttt
     5881 cagagccacg ctatgatgac ctcccgcagc gattgagacc cagtcagtgt cagcccctat
     5941 ccgaacagga gtcaatctgt cagtcgttgt gccgtctccc atttggccca gattgttgag
     6001 tccccacgcc cacaatgatc cgtccgcttt gagagctacg ttgtgggagt atcccatggc
     6061 aattgagacc caattagtat cagtccctat ctgaaccgga attaagctgc cagttcttgt
     6121 gctatccctc aacttgacgt cactgaaaca tccccacacc cacaaagtcc cgtctgattt
     6181 tagggccaga ctacgcacat cacttgttgc gatagctctc cacctgttgt cggttcctac
     6241 cctcacaggc gtcaatctgt cagtcgttgt gccgtctccc aattggccca gattgttgag
     6301 tccccacgcc cacatggacc catccgcttt cagggctatg gtgtgataat atcccgcagc
     6361 aattgagacc catgtggtat cggtccctat ttggacggga gtcagtctgt ttgttgtggt
     6421 gccgtcccct aattggccaa aattgttaag gccccacgcc cacatagacc cgtctggttt
     6481 cagggccaca gtgtgatcgt atcccgtagc aacagagatc cacgtactat caattcctat
     6541 ctgaaaagga gtgaagctgt caaaaggagt cagtcgatca attggtgagc catctcccaa
     6601 ttggccagtt tggttccatc cccatgccca caaagaacca tctggcttca ccactaccgt
     6661 gtgatcccat cctgcggcta tcttgggggt tactgcaaaa gaagttccct ctaaaaccgg
     6721 gaagatacag agagcacaga taagcaaaac agagaagaca
//
LOCUS       DATKOU010000230         8076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4937173, whole genome shotgun sequence.
ACCESSION   DATKOU010000230 DATKOU010000000
VERSION     DATKOU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4937173"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VMU21_10935"
     CDS             complement(<1..398)
                     /locus_tag="VMU21_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HUO78077.1"
                     /translation="MTDERPTEFQRFNLTFRIQHVILVVTFLLLAFTGWSLKYPEPAL
                     EYTGWWIRLWGGPKTAGIIHRTAGLIMIVDFIWHVIYLIYHLITGRMKFDPATTIVPV
                     PSDITNAIQNMLYFIGLGKTKPKFARYNYI"
     gene            complement(391..1326)
                     /locus_tag="VMU21_10940"
     CDS             complement(391..1326)
                     /locus_tag="VMU21_10940"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUO78078.1"
                     /translation="MSRALVIALLIVPLIGFAQIASAESFTCLACHGAMKGKIKTDKG
                     SFIEVNIDEERFSKSVHGGIECTVCHKTFKDNPHEAPKGEVPKNVSELASLVSSKAKI
                     DPVAYAACSECHGDIYTQVSQSIHGRNIMEKKQSDGALCNDCHGSPHYVAPAKSKASL
                     VNKWNVVKTCGECHQNEAIAKKYNLGTHIIEKYEESFHGKKHKLGHAGVPTCVDCHGA
                     HGIKKWDDPASPVAWGNRVVMCGKCHPGANKKFVASITHKPLGKDNPIPYFAEKGLII
                     LTVSTIGFVILHVFLEAYADIRDRVFRKGKEDSHD"
     gene            complement(1509..1844)
                     /locus_tag="VMU21_10945"
     CDS             complement(1509..1844)
                     /locus_tag="VMU21_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78079.1"
                     /translation="MKARKEMVRMGTKVGGVLGAIAFLVFGIVPGFYFGSYGILVLMS
                     KLFGGPLEATVLVRLATAAGILVGIVCVASVTIVVGAIFGTVVGYLLEAVSAPAEVKT
                     SEAAAIKAK"
     gene            complement(1915..2232)
                     /locus_tag="VMU21_10950"
     CDS             complement(1915..2232)
                     /locus_tag="VMU21_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78080.1"
                     /translation="MKTKEMARKMAYIGAGAGLVLFAIVGLLPGSFLGGVIGLNIAGS
                     LFGMPVGSALLPRLIVGISMLLGVLVSGIIFIAGSSIIGWLIGYVIDSLKVGKAAPVE
                     VKK"
     gene            2567..4786
                     /locus_tag="VMU21_10955"
     CDS             2567..4786
                     /locus_tag="VMU21_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUO78081.1"
                     /translation="MTSLRKILTTFILLAVSGWIPAADAATKDSGERSRPKIGLVLGG
                     GGAKGAAHIGVLKVLEEQHIPVDYIAGTSMGAIVGALYASGLSADELEKVLTSIDWDD
                     IFSGDPERKDVDWRRKREDYQFLTKLELGVKGGKILMPKGIIKDQKVNVLFETMMLHA
                     SGIDDFDKLPVPFRAVATDLETGEMVVLRSGRLADAARASMSVPGVFPPAEINDRFLI
                     DGGIVRNLPVDVVRQMGADIVIAIDVGKPLANREELQNALSIMNQMLDIMMKKNVQEQ
                     IDSLRPVDIYINPDLGDITSGDFARGKEAAATGEKAARQKIDVLKQYATGEQEYAAFL
                     ARHHRDLVTSVQVASVNIEVKGDTPIPPELIASRLSIKPGDTVDVKTMEHDLDRVYGM
                     KDYERVDLKVRQDGSEYDLAVQATEKSWGPNYLRVGMSLSSDFKGDSSYTLLVDYTRR
                     WVNQLGAEWKTQAQVGNTNSIYSEFWQPLDRTRFFFVMPHAEWRQNLYDVWINNTREA
                     QYRITRYDAGLDAGIQLSVYGEAAVGIVTGKLRAYRSTGQVDLPENEAQIGAITGRVL
                     VDQIDNPNFPNKGYWGRLTLFSSLKQLGADDAYNKVEGIVGAAYTYKRQTFLVSLDAG
                     SYIGPSLPFYDDFSLGGFLKLSGLSLDQLHGQQITLAKIVTYHNMAKSFFGDFYLGGS
                     LETGNVWQVDEKPSFSKLRLAGSVFVGYDTILGPLYLAYGRADGGFSAAYLYLGRIF"
     gene            5042..5326
                     /locus_tag="VMU21_10960"
     CDS             5042..5326
                     /locus_tag="VMU21_10960"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HUO78082.1"
                     /translation="MTTFFMFGKYSSEAVKEIHPERTRKSLKIIKAMGGKVKGMYALL
                     GDYDLVLIVECPDIDTIVKISIELHMMTGIHFSSLPAMPVDYFDALISKK"
     gene            5438..5884
                     /locus_tag="VMU21_10965"
     CDS             5438..5884
                     /locus_tag="VMU21_10965"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HUO78083.1"
                     /translation="MNEIIIVADLGHMKAFKMTRNPLGLESPKIELIRSCAFIEAHAK
                     SSDKFSDSAGRYYVGSGKKGTGAGFGEPHNIESEAEKRFITQFAEDIKTLILKEGCEK
                     WYFAADSGINGRIMEKLDSSIKAKLKKNIIANLTKATKPKILSRFA"
     gene            complement(5958..>8076)
                     /locus_tag="VMU21_10970"
     CDS             complement(5958..>8076)
                     /locus_tag="VMU21_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO78084.1"
                     /translation="HQLSRIIENLHHTEKALRDAQQTLEMKVAQRTAALAKANEELRK
                     EIAERTKAENELFESRERYRELVDGANSVILRWNRQGTILFMNPYGLDLFGYAQKELF
                     GKNLMDTIVSPSSQKDADLGTIINAIFTNPEDFSKNGNICKDGRKVWINWTNKAIFNE
                     AGEHVEILSVGNDVTQRRIIGQQLKESEERLRLALAAAHQGLYDLDLRTGVAKTNAEY
                     ALMLGYDPSTFEETNQKWMDRLHPDDRERVTAVYRGYLDGEIPEYKVEFRQKTRAGDW
                     KWILSLGRIVERDADGRPLRMLGTHTDINDRKQAEEERLDLERKLLHAQKLKSLGVLA
                     GGIAHEFNNLLLTILGNLDLALMKLTPISPAHEYVDRAVQAAHCSAGLAAQMLAYSGK
                     GTFVVQSINLSELVEAISHLLESSLTKTAKLVMNLQRDIPDIDADVAQIQQVVMRLIT
                     NASEAIGDDLGVITLSTGVQECTEAYLQKNRLADKLLPGRFVYLEVSDTGSGMDANTQ
                     QHMFDPFFTTKAVGRGLSMPAISGIVRGHKGGILVESVKGQGTSVRLLFPISASAPEI
                     APGKAARDEMQEESCPIPNHGTVLIVDDEEMVRKVCRAMVEHLGFSVLTAEDGIKAVE
                     IFQARSSEIDCVLLDLSMPKKDGLATYEDIIRIKPDTKVILSSGFHEGEASSRFSAKG
                     LAGFIQKPYRLATLRSTLYNIMF"
BASE COUNT         2018 a         1980 c         2013 g         2065 t
ORIGIN      
        1 tggatatagt tgtatcgagc gaatttgggt ttcgttttgc ccaggccgat gaaatagagc
       61 atgttttgta tcgcatttgt gatatcactg gggacaggaa caatggtggt tgccgggtca
      121 aatttcatcc tcccggtgat aagatggtag atcaggtaaa tgacatgcca gatgaaatcg
      181 acaatcatga tgagtcccgc cgtgcggtgg atgatacccg cggtctttgg tcctccccac
      241 agccgtatcc accagcccgt gtactcaagg gcaggttcag ggtacttgag ggaccagccg
      301 gtaaaggcaa ggagcaggaa tgttacgacc aggatgacgt gctggatcct gaaggtgaga
      361 ttgaatcgtt gaaattctgt cggtctttca tcagtcatgg gagtcctcct tccccttcct
      421 aaacacacgg tccctgatat ccgcatatgc ctcgagaaag acatgcagga ttacgaagcc
      481 gatggtcgaa accgtaagga taatcagacc tttttccgcg aaatagggta tgggattgtc
      541 cttcccaagg ggtttatggg ttatggaagc cacaaacttc ttgttcgctc ccggatggca
      601 ctttccgcac atgacgaccc ggtttcccca cgcgacgggg gatgcggggt catcccattt
      661 cttgatacca tgggcaccgt gacaatcaac acaggtgggc accccagcgt gtcccagctt
      721 atgttttttc ccatggaaac tctcttcata cttttctatt atatgagttc ccagattgta
      781 cttctttgca atggcctcat tttggtggca ttcaccgcag gtctttacca cattccattt
      841 gttcaccaag gaggcctttg atttagccgg tgcaacataa tggggtgagc cgtggcagtc
      901 attgcagagc gcgccgtcag actgtttctt ctccatgatg ttcctgccgt gaatgctctg
      961 tgaaacctgt gtataaatat caccatgaca ttcactgcat gcagcatacg ccacggggtc
     1021 tatttttgcc ttggaagaga cgagacttgc cagttctgaa acattcttgg ggacttctcc
     1081 ctttggcgct tcatggggat tatccttgaa tgtcttatga catacagtgc attcaatccc
     1141 tccgtgaaca gatttggaaa agcgttcttc atcaatattg acttcaatga aggatccctt
     1201 atcagtcttt attttgccct tcatggcgcc gtggcaggca aggcaggtga aactttcggc
     1261 gcttgcgatc tgggcaaaac ctatcagggg gacaatgagt aacgcaatca cgagagccct
     1321 gctcatgaga cctccttttt tttgcctaaa aggtataagg ctattcacaa tagccaagag
     1381 attatacaca aaaaagctgg ttttggaaat aataaaaaaa agcatatctg gaagtttagg
     1441 agagatgggg tcaatagggc aataaaaaag gggctttccc tcctctttta ttgccctgac
     1501 ttaagcgatt attttgcctt aatcgcagcc gcttctgagg tcttgacctc tgccggagcg
     1561 ctcactgcct ccaggaggta tcccacgacc gtaccgaata tggcacccac cacaatggtc
     1621 actgatgcca cacagacgat acccacaaga atgcctgctg cagtggccag cctcaccagc
     1681 accgttgcct ccaagggccc tccgaacagc ttgctcatca gcaccaggat cccgtaactg
     1741 ccaaagtaaa accccggcac tatcccgaac accaggaacg caatcgctcc cagcactcct
     1801 cccacctttg tccccatcct taccatctct tttctcgctt tcatcttcct gtcctccttt
     1861 tctttagttg ttttttttac tatttactat tcactgttta ctttttactg tttattattt
     1921 ctttacctca accggggctg ccttgcccac cttcaaagaa tcaatcacgt atccgataag
     1981 ccagccaata atggatgaac ccgcaataaa gatgatccct gataccagca cacccaggag
     2041 catggagatg cccacaatca gcctgggaag cagggcagaa cccaccggca tgccaaagag
     2101 gctccctgca atattaagtc cgatcacccc gccaaggaaa gagcctggca ataaccccac
     2161 tattgcaaac agaaccagac ccgcccctgc tcctatgtat gccatctttc ttgccatctc
     2221 ttttgttttc atctttatgt cctcctttat tttgacttca ccccatataa agcaccttcc
     2281 atgccagata aaaacatatt gaaattaaaa gaatattata actaaacctt ttgcagaact
     2341 gagaaaacta ttcctttctt ttgcaggtgt gcaaaaaacc aacctctagt ggacctgaga
     2401 tggaccgtcg cgaccatggt gtccaaaaaa actcatgttg atttgtcatt ttgaatggat
     2461 ttaagaacag atatgtttta gaatgtaacg ctatctagga atcgagcaga ggaaaaaata
     2521 atggttataa tttttttaga acagcaaaag gaaatccggg cctgacatga cttcactgag
     2581 gaaaatactg accactttta ttctccttgc tgtttcggga tggattcccg cggctgatgc
     2641 tgctaccaaa gattcaggag aacggtcccg tcctaagatc ggccttgtac ttggtggcgg
     2701 gggtgcaaag ggagctgccc atattggcgt attgaaggtc cttgaagagc agcatatccc
     2761 tgttgactac attgcaggta ccagcatggg cgctattgtg ggtgcgcttt atgcatcagg
     2821 gctgtcagcc gatgaactcg aaaaagtttt gaccagcatt gactgggatg acatcttttc
     2881 cggtgaccct gaacgcaagg atgtagactg gcgcagaaaa cgggaggact accagttcct
     2941 caccaaactc gagttagggg tgaagggtgg aaagatcttg atgcccaagg gcatcatcaa
     3001 agaccagaag gtgaatgtcc tcttcgagac aatgatgctc catgcctcag gtattgatga
     3061 tttcgataaa cttcctgtcc cctttagggc cgtagccaca gacctcgaga ccggcgagat
     3121 ggtcgtgctc aggagtggaa ggcttgctga tgcggcaagg gcgagcatgt cggtccccgg
     3181 ggtctttccc cccgcggaga taaatgaccg ttttctcatt gacggcggta ttgtgcggaa
     3241 cctgccggtg gatgttgtgc gacagatggg tgcagatatc gtcatcgcga tagacgtcgg
     3301 gaagcccctt gcaaaccggg aagaattgca gaatgcgtta tctattatga accagatgct
     3361 cgatatcatg atgaagaaga atgtccagga gcagatcgac agcctcaggc cggtcgacat
     3421 ctacattaat ccggaccttg gtgatatcac ttccggagat tttgcacggg gtaaggaagc
     3481 cgctgctaca ggtgaaaaag ctgcacgtca aaagatcgat gtgttgaaac agtatgcgac
     3541 gggcgagcag gagtatgctg cattccttgc gaggcatcac cgtgacctgg ttaccagtgt
     3601 gcaggtagct tcagtgaata tcgaagtcaa aggagatact cctatccccc ctgagcttat
     3661 tgcaagcagg ctgagtataa agccgggtga cacggtggac gtcaaaacca tggaacatga
     3721 cctcgaccgt gtctatggca tgaaagacta cgagcgggta gacctcaagg tcagacagga
     3781 tggctcagag tatgaccttg cggtgcaggc taccgagaaa tcctgggggc cgaactactt
     3841 gcgcgtgggt atgtcgttaa gtagcgattt caaaggagac agcagctata ccctccttgt
     3901 ggactatacc agacgatggg tgaatcagct tggcgcagaa tggaaaactc aggcgcaggt
     3961 cggcaacacg aacagcatct actcggaatt ttggcagccc cttgaccgta ccaggttctt
     4021 ttttgttatg cctcatgccg agtggaggca gaatctttac gatgtctgga ttaataacac
     4081 gagggaggcc cagtaccgta taacaaggta cgatgccggc cttgatgccg gaatccagtt
     4141 atccgtatac ggagaggcag cagtgggtat tgtgaccggg aaactgaggg cttatcgcag
     4201 cacaggacag gttgatctac ccgaaaacga ggcccagatt ggtgcgatca ccgggcgtgt
     4261 tctcgtggat cagatcgata atccaaattt ccccaataaa ggatattggg gaagactgac
     4321 actcttttca tcactgaagc agctgggtgc ggatgatgca tataacaaag tcgagggcat
     4381 tgtcggggcg gcatatacct ataagaggca gaccttcctc gtcagcctcg atgcaggatc
     4441 gtacatcggc ccgagcctgc ccttctatga tgatttttct ctcggggggt tccttaaact
     4501 ttccggacta agcctggacc agctgcatgg ccagcagata acactcgcca agatcgtcac
     4561 ctatcacaat atggcgaagt catttttcgg cgacttctat ctgggcgggt cactagagac
     4621 cggtaatgta tggcaggtcg atgagaagcc ttctttcagc aaactccgtc tggcgggaag
     4681 tgtcttcgtc ggttatgaca caattctcgg tcccctctat ctcgcctatg ggcgtgctga
     4741 cggaggtttc agtgcggcat atctttacct tggccggata ttctgaaata tcccatgtct
     4801 tctgatcctc gcctctgagt tccctgtaag acagggtgcc tgcaggaaag cagtaaaagt
     4861 cacatttctt tcctaaagat atctcactat gggcttttag tggacagagg gacaatttta
     4921 tggtacttat taagtaagaa gggtgtcctg gaacgctgaa ccttggtttt tataagggag
     4981 gtcaatgtca atcagcagcg gatgaatgga gcagagaagc acacacagga aggaggctcg
     5041 tatgacaaca ttttttatgt ttggcaagta ctcgtctgaa gcggtgaaag aaatccatcc
     5101 tgagcgaacg aggaaatccc tgaaaatcat taaagccatg ggtggaaagg taaaggggat
     5161 gtatgcactc ttgggcgatt atgacctggt gctcatcgtt gaatgtcctg acatagatac
     5221 catagtaaag atttccatag agttgcacat gatgacagga atccattttt ccagcttgcc
     5281 tgcaatgcct gtggactact ttgacgcatt gatctcgaaa aagtaggacc ccgtatcgtg
     5341 aggaaatttg cgcatgagtt gaatggaagg aaaatgaggg ccatgtgcga tgcgtgaaga
     5401 tgttctctct tgaatattcc atggaaagga gaatgcgatg aacgaaataa ttattgttgc
     5461 agacctcgga catatgaagg catttaaaat gaccaggaac cccctcgggc tggagagccc
     5521 gaagattgaa ttgataagga gttgtgcctt tattgaagcc catgcaaaat cttcagataa
     5581 attcagtgac tcagcaggga gatactatgt ggggagcgga aaaaaaggga caggtgcggg
     5641 atttggtgag ccccataata ttgaatcaga agcagaaaaa cggttcataa cccaattcgc
     5701 agaagacata aagaccctta tccttaaaga gggttgtgag aaatggtatt tcgctgcgga
     5761 cagcggcata aacggccgga tcatggaaaa gcttgattct tccataaagg caaagcttaa
     5821 aaagaatatt attgcaaatc tcaccaaggc caccaaaccc aagattctga gccgctttgc
     5881 ctgacgatcg aaggcaagac ctttcaggac aaggggcaca tcgcaaggtg agcatcgagt
     5941 tggggtgttc ccgtatttta aaacattatg ttatacaggg tggaccgaag ggttgccagc
     6001 ctgtagggct tctggatgaa cccggccaaa ccttttgcag agaaccggct ggaggcttct
     6061 ccttcgtgga atccgcttga gagtatcacc tttgtatctg gtttgatacg tatgatatcc
     6121 tcataggtcg ccaagccatc ctttttcggc attgacaggt caagcaacac gcagtcaatc
     6181 tctgatgacc gcgcctgaaa aatttccact gccttgatcc cgtcttctgc ggtgagcacc
     6241 gaaaaaccaa gatgctcaac cattgccctg cacacttttc ggaccatctc ttcatcatcc
     6301 actataagga ccgtcccgtg gttcggtatg ggacaagact cttcttgcat ctcatcacgt
     6361 gcagcttttc caggggcaat ctctggtgca gatgccgaaa tggggaacag gagccttacg
     6421 gaggtacctt gtcccttcac actttccaca aggatgcccc ctttgtgccc gcgaacaatg
     6481 ccagagatcg cgggcatact cagtccacgc cctacggcct ttgtggtaaa aaaagggtcg
     6541 aacatgtgtt gctgggtgtt ggcatccatt cctgagcctg tgtcagatac ctccaggtac
     6601 acgaatcggc cgggcaacag cttatcggcg agcctgttct tctgcaagta cgcctcagta
     6661 cattcctgca cccctgtaga aagcgtaatg accccgagat cgtcccctat cgcctctgag
     6721 gcattggtta tcaatctcat gactacctgt tgaatctggg ccacgtcagc atcgatgtcc
     6781 gggatgtctc tctgcaggtt catgacgagt tttgcggttt tcgtgagaga agactcgagg
     6841 agatgggaga ttgcttccac aagctcactt agattgatac tctgaaccac aaatgtgcct
     6901 ttccccgaat aggcaagcat ctgggccgcg aggccagcgg agcaatgagc tgcctgcacg
     6961 gcacgatcga catactcatg tgcaggggaa atgggagtga gtttcataag cgcgaggtcg
     7021 agatttccaa ggatcgtcag caaaaggttg ttgaactcgt gggcgatccc tccggcaagc
     7081 actccaaggc ttttgagttt ttgggcgtgg agcagtttcc gctccagatc cagacgctcc
     7141 tcctctgcct gtttacgatc gttgatatcg gtatgggtac cgagcatacg aaggggtctg
     7201 ccgtctgcgt cacgttccac tatcctcccg agggaaagga tccatttcca gtctcctgct
     7261 cttgtcttct ggcggaattc cactttatat tccggtatct cgccatcgag gtacccgcga
     7321 tagactgctg tgacgcgctc cctgtcatcg gggtggagac gatccatcca tttctggttg
     7381 gtttcttcaa atgtggatgg gtcatagccg agcatcaatg catactcggc gtttgtcttt
     7441 gctacgccgg tacggagatc cagatcgtac aaaccctgat gcgcagcggc aagcgcaagc
     7501 cgcagccttt cttcgctttc cttcagttgc tgtccgataa ttctccgctg ggtcacatca
     7561 ttgccaaccg agaggatctc cacatgttca cccgcctcgt tgaaaatggc cttatttgtc
     7621 cagttgatcc agaccttacg gccgtccttg caaatatttc cattcttgga gaaatcttcc
     7681 gggtttgtaa aaatcgcatt gattatggtc ccaaggtcag catccttctg tgatgagggc
     7741 gatacgatgg tgtccatcaa gtttttaccg aacagttcct tctgcgcata tccaaagaga
     7801 tcgagaccat agggattcat aaagaggata gttccctgcc tgttccacct gagaataaca
     7861 ctgttggcgc cgtccacaag ctcacggtag cgttccctgc tctcaaaaag ttcgttctct
     7921 gcctttgtcc tctcggcgat ctcttttcga agctcttcat ttgccttggc aagggcggct
     7981 gtccgctgtg ccaccttcat ttcaagggtc tgctgggcat cgcgcaaggc cttttctgtg
     8041 tgatgaaggt tctcgatgat ccttgataac tggtgg
//
LOCUS       DATKOU010000231         5597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3388335, whole genome shotgun sequence.
ACCESSION   DATKOU010000231 DATKOU010000000
VERSION     DATKOU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388335"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VMU21_10975"
     CDS             complement(<1..186)
                     /locus_tag="VMU21_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78085.1"
                     /translation="MKEASTGKKTLGKKGGEKRMKRLIAVMVTVVFVLGFGVLGFAAD
                     MDKCGSCHKGDKALDKIV"
     gene            complement(217..906)
                     /locus_tag="VMU21_10980"
     CDS             complement(217..906)
                     /locus_tag="VMU21_10980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF008602.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUO78086.1"
                     /translation="MRSVWMAVLAILVMAGSVIAEEPIALKSQKDKISYIIGLDIGNN
                     LKRQGAEIDPDTLLRGMKDALSGSKPLLSEDEIREVTTAFRQEMAQKQAEVTKKLAEK
                     NKSEGEAFLGENKKKEGVKILPSGLQYKVITEGSGRSPKESDTVTVNYKGTFVDGTEF
                     DSSYKRGEPATFPVNGVIKGWTEALPLMKEGAKWQLFIPSSLAYGEAGAGNAIGPNAT
                     LIFEVELISIK"
     gene            complement(1234..1587)
                     /locus_tag="VMU21_10985"
     CDS             complement(1234..1587)
                     /locus_tag="VMU21_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HUO78087.1"
                     /translation="MAAKKPAAKKKTVKKVAKKVAKKPAAKKKTVKAKRKPNPAFMKP
                     MKISDTLAKVVGSKPLPRTEVTKKLWQYIKKNKLQDSVNKRNINADENLKAVFGGKKT
                     VSMFEMTKLVNKHLS"
     gene            1831..2082
                     /locus_tag="VMU21_10990"
     CDS             1831..2082
                     /locus_tag="VMU21_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78088.1"
                     /translation="MPRVITYECTQCGSEVVVTESLESELSPIYCCGIEVAEIAAKEK
                     KPARPKKKAVKKVTKKPAPKKRAAAKKKPAVKRKASRKS"
     gene            complement(2098..2499)
                     /locus_tag="VMU21_10995"
     CDS             complement(2098..2499)
                     /locus_tag="VMU21_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HUO78089.1"
                     /translation="MAVRVVCAGTFDHLHPGHISFLNQAKALGSELIVIVARDKTVRR
                     IKGITPDHDEQRRKHDVERAGIADRVVLGNEDEDIFSILHILSPDIVALGYDQRISEE
                     KITKRFPGCGVIRLEPFHPEKFKSSLYRSER"
     gene            2644..2922
                     /locus_tag="VMU21_11000"
     CDS             2644..2922
                     /locus_tag="VMU21_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78090.1"
                     /translation="MKNKHTEQKERSAFPTHSVAIIKEAVEQGASFDQACSLVAIKDP
                     ELREVVFNSALKALVSDMHLSRGMPLKQLAMKLGLSLSRVINAKERIL"
     gene            complement(3006..4709)
                     /locus_tag="VMU21_11005"
     CDS             complement(3006..4709)
                     /locus_tag="VMU21_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUO78091.1"
                     /translation="MSEREREAGQETARDLPEPLALIPICAWCKKIRNERAGWEDIEV
                     YFTREGLGEFTHSICPVCAEKIFSKRVYLESYQTICKAISRSLALDEVLNVIATNVVK
                     VMNVKASSLRLLNKETQQLELVAFHGLSERYAHKGPVAHDGSIVDALAGKPVSIYDIT
                     ADENAKYRKEALDEGIRSILSIPMRFGEEVIGVLRMYTSEPVEYSEEDLKFVAAIAEQ
                     AAIAIVNARVFEKTVSKEKEYLRVFHRVTRAVSSSLQLDEVLNMIVRTLPEVMGLKAA
                     TIRLLDDSGTHLHLVAAHGLSERYLSRGPVDMEENIREALEEKPVAIHDVSKDPRVHY
                     GKEAEEEGIKSMLTLPIIARGKVIGVLRLLTAHLRHFSPQDIDFSAALAEQCGTAIEN
                     ARMYERQYKEAKYLKALQDIAKAVSSTLDVREVMDMIVTRIAHAMNTDAATIRLLDPS
                     QKKLELVASYGLSEKYLNKGPVDAEKSVADALKGNPVAIHNVSSDPRIVYQSEAADEG
                     VRSMLVIPIIFRGRVIGVLRALTKKEHRVFHQDEIEFATSLAEQAAIAIDHAKASSPN
                     V"
     gene            4851..5207
                     /locus_tag="VMU21_11010"
     CDS             4851..5207
                     /locus_tag="VMU21_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78092.1"
                     /translation="MPEGSENRQFSEEDNRAYRKSIEMIRTNLENGVKFDLACEFITV
                     EDRELRELIIDDALKIEIAELHYGKQMPLQEVSKRLGVSVDRLLKASNEMLEDVVQTT
                     EKFSGWGSGDKGPMTH"
     gene            5215..5478
                     /locus_tag="VMU21_11015"
     CDS             5215..5478
                     /locus_tag="VMU21_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HUO78093.1"
                     /translation="MQRVTLFSLSSCPVCKKVKKFLEDNHVSFTLIEVDMLDSGEQWL
                     MTKKLKEYNPQATYPTVVMEEVIRGFDVEALSIKLLGDKPRQS"
BASE COUNT         1312 a         1534 c         1267 g         1484 t
ORIGIN      
        1 cacaatcttg tccagtgcct tgtccccctt gtggcaggaa ccacacttat ccatatcggc
       61 tgcaaagcca aggaccccaa aaccaaggac aaagactacg gtgaccatca ctgcgataag
      121 tctcttcatt ctcttttcac cccctttctt tccgagcgtc ttcttaccag ttgacgcttc
      181 tttcatgtgc tctcgaaatc cctttttctc aatagtttat tttattgaaa tcagctcaac
      241 ctcaaagata agcgttgcat tgggccctat ggcatttccc gcacctgctt ctccgtacgc
      301 aaggcttgaa ggaataaaga gctgccattt tgcaccctcc ttcatcagcg gcagtgcttc
      361 tgtccacccc ttgatcaccc cgtttacagg gaaggttgcc ggttctccac gcttgtaaga
      421 actgtcaaac tcagtcccgt ccacaaaggt gcccttatag ttcactgtca ccgtatcgga
      481 ttccttcggt gacctcccgc tcccttcagt gattaccttg tactgcagcc cgctcgggag
      541 gattttcact ccctcctttt tcttgttttc cccgaggaac gcctctcctt cgctcttgtt
      601 cttctcagcc agtttctttg tcacttctgc ctgtttttgt gccatttcct gcctgaatgc
      661 agtagtgacc tcacggattt catcttcgct caataagggt ttactgccgg acaacgcgtc
      721 cttcatgccc cttaggagtg tatccgggtc aatctctgca ccttgccttt tgaggttgtt
      781 tccaatatcc aggccaatga tataacttat cttatccttc tggcttttca atgctatggg
      841 ttcctccgca ataacgcttc ccgccataac cagaatcgcc agcacagcca tccaaactga
      901 tctcatcgat tctcctttct tcaaaaacac tttcccgcca atcgagctca cctattttat
      961 gcatttgatg cgtggaattc aataccaaaa taaggcctcg tgaatccctg ccccccgaac
     1021 gccaggcctg gtcctgcatt gaagataccc tacggcagag accgcagggt atcttaacgt
     1081 aaggaattgt tttttatatt cgctcgctaa ccctgcgcta agccgaaggg aatgtgctcg
     1141 ctatccgttt caaaaaaaag gcaggtgagg aacgcacctg ccccggactt tgatcctctt
     1201 tatacgcctg tataactcag tactggatgc agactagctg agatgtttgt tcaccagctt
     1261 ggtcatctcg aacatgctga cggtcttctt tcctccaaac actgccttca aattctcatc
     1321 cgcgttgatg ttcctcttgt tcacgctatc ctgcagcttg ttcttcttga tgtattgcca
     1381 taatttcttg gtcacctctg tcctcggaag tggtttgctt cccacgacct ttgccagggt
     1441 gtcactgatc ttcatgggct tcatgaacgc agggttcggt ttccttttgg cctttaccgt
     1501 ctttttcttc gctgcgggct tctttgccac tttctttgcc accttcttca ccgtcttttt
     1561 cttcgctgcg ggcttctttg ctgccatctc tccctccttt acatttttta gcatctcgcg
     1621 tggatgccat agaatacatt gttcaaccaa aaaaatccta ccaaacatca cacacattgt
     1681 caacaacttt ctgccctcca caaaaccgct gtcgcagagt ggaaagatcg aggatccttt
     1741 ccagggagaa ttttcgtaga ttcagacaca caaaaaatga tatagtaaac aatattcaca
     1801 ttatctaacc ttaaccaagg agggagagac atgccaaggg taatcactta tgaatgcacg
     1861 cagtgcggtt cagaggttgt cgtcacagaa tctctggaat ctgaattaag tcccatttat
     1921 tgctgcggaa tagaggtggc agagatcgcc gcaaaggaga agaaacctgc acgtcctaag
     1981 aagaaggctg taaagaaagt gaccaagaag cccgcaccta aaaagagggc cgcagcaaag
     2041 aaaaagcctg cggtaaagag gaaggcgtcc agaaaatcct gagagaaccc gtacgaatca
     2101 ccgttcggac cggtaaaggg aagatttgaa tttctcggga tgaaagggct ccagacgaat
     2161 caccccgcat cccgggaacc tctttgtgat cttttcttca gatatccgct gatcataacc
     2221 aagggccact atgtctgggg agagtatgtg gaggatggaa aagatgtcct catcctcatt
     2281 acccagaacc accctgtcgg cgattcctgc ccgttccaca tcgtgtttgc ggcgctgctc
     2341 atcatggtcg ggagtgatgc cctttatcct cctgaccgtc ttgtcccggg caacaatgac
     2401 gatgagctca ctccccagtg cctttgcctg attgagaaag ctgatatgcc cgggatgaag
     2461 atgatcaaag gtccccgcac acacaaccct taccgccatt cagccaatct ccgttccctc
     2521 atgaggtcac caccttcaac actacgcata ttgtattcaa aaagggcaaa atgatacaat
     2581 atgcataata catacacccc gatgaatgcc tctcctttct gaggacgtcg gacgtcaaat
     2641 accatgaaga acaaacatac tgaacagaaa gagaggtctg ccttccccac gcattccgtt
     2701 gcaattatca aagaagcggt ggagcagggg gcgtcttttg accaggcatg ttcactcgtg
     2761 gcgatcaagg acccggaact cagagaggtt gttttcaaca gcgccttgaa ggcccttgtt
     2821 tccgacatgc acctctccag ggggatgcca ttaaagcagc ttgccatgaa gctggggctg
     2881 tcattgagcc gtgtgataaa cgccaaggag cgtatcctct aggatgggga agtcgctgtt
     2941 tcggataagg tggccggcat cagcggttcc cggggcattg tttcataacg tataaagaca
     3001 aatcctcaca cgttcggtga ggatgccttt gcatggtcta tagcaatagc tgcctgttct
     3061 gcgagggagg tggcaaattc gatctcgtcc tgatgaaaca ccctatgttc tttcttggta
     3121 agtgccctca gcacgccgat caccctcccc ctgaagatta tggggatcac gagcatactg
     3181 cggacaccct catctgccgc ttcagactga tagacaatcc tcgggtctga ggagacattg
     3241 tggatcgcaa ccgggtttcc tttcaaggca tctgccacac tcttttccgc atccaccggt
     3301 cccttgttca gatatttttc gctcagcccg taagaggcaa caagttcaag tttcttctgt
     3361 gaagggtcaa ggagtctgat ggtggccgcg tcggtattca tcgcatgagc tatcctggtc
     3421 acgatcatgt ccattacctc acgcacatcc agtgtggaac tcactgcctt tgcgatatcc
     3481 tgcagggctt taagatactt ggcttcctta tattgcctct cgtacatcct cgcattttct
     3541 atggcagtgc cgcattgctc tgcaagggct gccgaaaaat cgatgtcctg ggggctgaag
     3601 tgtctcaggt gtgccgtgag gagcctcagg acccctatta ccttccccct tgcgatgatg
     3661 ggcagcgtga gcatgctctt tattccttcc tcctcggcct ccttaccgta atgcaccctc
     3721 ggatccttcg agacgtcatg gatcgcgacc ggtttttctt ccagtgcctc tcttatattt
     3781 tcttccatgt ccaccggtcc ccgactgaga tacctctcac tcaacccatg ggctgcgaca
     3841 aggtgaaggt gtgtccctga atcgtccagg agccgtattg ttgctgcctt gagacccata
     3901 acctcgggga gcgttctcac aatcatattc agtacctcat ccagctggag ggatgaactc
     3961 accgccctgg tgacccgatg gaagacccgg aggtactctt tctcctttga aacggtcttt
     4021 tcgaagaccc tcgcgttcac aatagcaatg gctgcctgct ccgcaatggc ggccacaaat
     4081 ttcaggtcct cctccgaata ttcaaccggc tccgaggtat acatcctgag caccccgatc
     4141 acctcttccc caaaacgcat gggtatggag aggatgctcc gtatgccctc atcgagagcc
     4201 tctttcctgt acttcgcatt ctcgtctgcg gtgatgtcgt atatggagac cggcttccct
     4261 gccaatgcgt ccacaatact tccgtcatgc gccacaggtc ccttatgagc ataccgctcg
     4321 ctcagtccat gaaaggcaac gagttccagt tgctgcgttt ccttgttcag gagtctgagg
     4381 gaactcgcct ttacgttcat cactttcacc acattcgtgg cgatgacgtt cagcacctcg
     4441 tcaagggcga gactcctgct gatggccttg caaatcgtct ggtagctttc aagatagacc
     4501 ctcttggaga atatcttttc ggcacagacc ggacagatgg agtgggtgaa ttcacctaat
     4561 ccctccctgg taaaatagac ctctatatcc tcccatcctg cacgctcatt ccttattttc
     4621 ttgcaccatg cgcagatagg aatcaatgcc agaggctccg gaaggtccct cgctgtttcc
     4681 tgtcctgcct ccctctctct ttcgctcaca gaattccccc cacacacaca gtaataagtg
     4741 ttcttattct tttcctgtga agagagcgat gtcaagggat tttatataaa ttcttttaat
     4801 ggtcccattc ccgcaaaggg ccatcgttcc cgtatatgct ataatgattc atgccagagg
     4861 ggagcgagaa cagacaattc agcgaggaag acaaccgtgc ctacaggaag agcatcgaga
     4921 tgatacggac gaaccttgag aacggggtga agttcgacct tgcgtgtgaa tttatcactg
     4981 tggaggacag ggaattaaga gagttgatta ttgatgacgc cctcaagatc gagattgcgg
     5041 aattgcatta cgggaaacag atgcctctcc aggaagtctc gaagcggctc ggtgtttccg
     5101 tggaccgcct tttaaaggca agtaacgaga tgttggaaga tgtggtccag accaccgaga
     5161 aattctccgg ctggggatcc ggtgacaaag gaccgatgac ccactgagcg tgtcatgcag
     5221 cgcgtaaccc tcttctcttt aagcagctgt ccggtctgta aaaaagtcaa aaagttcctt
     5281 gaggacaacc acgtatcctt taccctcatc gaagttgata tgctggacag cggggagcaa
     5341 tggcttatga caaagaaact gaaggaatat aaccctcaag ccacctatcc caccgtagtg
     5401 atggaagagg tcatcagggg tttcgatgtt gaggcgttat ccataaaact tctgggggat
     5461 aaacccagac aatcgtagtg ggagggaaga agtttgtcac gggacaaact ctacgagagc
     5521 ctgaaacgat atgccgggtc acagggcatg gaattgaatg atgacaggga gttcgtattg
     5581 gatatcatcg atggcct
//
LOCUS       DATKOU010000232         3568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2235559, whole genome shotgun sequence.
ACCESSION   DATKOU010000232 DATKOU010000000
VERSION     DATKOU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235559"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VMU21_11020"
     CDS             complement(<1..883)
                     /locus_tag="VMU21_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUO78094.1"
                     /translation="MISKIIEYSARNKFIIFLMVIFLCVWGYWALTKTPLDAIPDLSD
                     TQVIIYTDWAGRSPDLVEDQVTYPITSTLLAAPKVQAVRGFSFLGSSFIYVIFEEGTN
                     IYWARSRVLEYLQSVRNKLPADVNPVLGPDATSVGWGFNYALVDEKRGHDLAQLRSLQ
                     DWNIKLALESVPGVSQVASLGGFVKQYQISIDPNRLSAYNVSLMKVMDAVRKSNNDVE
                     GRVLEMSGVEYMVRGRGYIKSVKDIEDIAVGTNGVGTPVFLRDVAHVQLGPEIRRGLA
                     DLDGKGEVTGGIVVVRFG"
     gene            complement(975..2231)
                     /locus_tag="VMU21_11025"
     CDS             complement(975..2231)
                     /locus_tag="VMU21_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUO78095.1"
                     /translation="MRKGFLITIGAVIFLVGLVIYLSRAGFVKVPVKEGGKEPSVSQQ
                     SPAQPSGEGGGEPSQTKPAAMNGQEKEQKETEEAPTVEIPQEKQRLIGVRTTEVSVRP
                     LQKIIRTVGRVEYDERRLATVNAKVEGWIERLHVDYTGKYVKKGEPLAEVYSPELVAT
                     QQEFLNALRWAKQGSETKSGDMNKMLLKDADAIVQAARERLKLWDISDDQIRRIEDSG
                     KAVRTLTIYSPVSGYVVQKMALQGVRVMPGEKLFDVADLSSIWVISDIYEYELPSTKV
                     GETAQIRLSYFPGKEFSSRIDYVYPTLSGETRTAKVRFSLPNPGGQLKPQMFTNVEVK
                     INLGKKLSIPDDAVIDTGTRQIVYVDKGDGYFEPREVMLGIRTEGFREVLMGLKAGEK
                     VASSATFLIDSEAQLKGVKPLEGHKH"
     gene            complement(2228..>3568)
                     /locus_tag="VMU21_11030"
     CDS             complement(2228..>3568)
                     /locus_tag="VMU21_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUO78096.1"
                     /translation="KCAAGSVRTRGIPDRIRSSSLFVFLPTYRTVRLLLTAYLVLCFS
                     YVFAEDLNLQDLIDEALKNNHEILITESRVAASEYRIPQVKSLPDPMLMFGYQNEGWK
                     KYTYGESIMAWWMFSASQTFPFPGKLSTKGEMAARETEGLRASYEGVRLRTIEKVKEL
                     YYDLLYTYKTIDIINERAALFTRIEDAAIARYSSGKGMQQEVLMAQTEKYMLLEKEQM
                     LKQRIQSLEAMLNSTIGRDINAPLGRPVEIKGTGLETSLNELLTLHVEHSPFVKEKER
                     MVASAQARVRMAELDYYPDFTVNAGYFNRGSDFDPMWSLTTTINIPIFYRTKQRQAVN
                     EARALLSGAEHELDSTKIMLSSTIRDNYSMARTAERLMELYKSGLIPKTYQDFESALS
                     GYGTGRVDEITVITRLKTLLDLEISYWGQFTEREKAIARLQSITGIAGSGDKEK"
BASE COUNT          784 a         1014 c          808 g          962 t
ORIGIN      
        1 ctccgaagcg gacaacaacg atccctccgg tcacctcgcc ttttccgtcg aggtctgcaa
       61 gcccccttcg tatctcgggg ccgagctgaa cgtgcgccac atctcgaagg aatacgggcg
      121 tgcctacgcc atttgttccc accgcaatat cctcaatgtc cttaacgctc tttatatacc
      181 ctcgtcctct caccatatac tctaccccgg acatttccag aacccgtccc tccacgtcgt
      241 tgttgctctt ccgcactgca tccatcactt tcatgagcga gacgttatat gcagagagac
      301 gattggggtc gatggagatc tggtactgct tcacgaagcc gccgaggctg gcaacctggg
      361 agacgcccgg cacgctttcc agggcgagct tgatgttcca gtcctggaga gaccggagtt
      421 gggcaagatc atggcccctc ttctcgtcca caagcgcgta gttgaagccc caccctacgc
      481 tcgtggcatc aggtccgagc acaggattga cgtccgcggg cagcttgttc ctcactgact
      541 ggagatattc gaggacacgg ctccttgccc agtagatatt cgtcccttcc tcgaaaatca
      601 catagatgaa tgagctgccg aggaaggaaa aaccgcgcac tgcctggacc tttggggcag
      661 cgagaagggt agaggttatg ggataggtta cctggtcctc cacgaggtcg gggctcctgc
      721 ctgcccaatc ggtgtatata atcacctgtg tgtcgctcag gtcagggatc gcgtcgaggg
      781 gagttttcgt gagagcccag tatccccaga cgcaaaggaa tattaccatc aggaagatga
      841 taaacttgtt ccgtgcgctg tattcgataa ttttggaaat catatatcag taagaagtga
      901 atagtgaata gtgaaacgtg agaaggaaac aatccctatt ctatttacct ttcactcttc
      961 acgattcacg gcctttagtg tttatgccct tccagaggct ttaccccctt cagttgcgcc
     1021 tctgaatcaa tgaggaatgt ggcggatgaa gcgacctttt cccctgcctt aaggcccatg
     1081 agcacctccc tgaacccttc ggtccgaatg ccgagcatga cctcccttgg ctcaaaatac
     1141 ccgtcaccct tgtccacata cacaatctgt ctcgttcctg tatcgatcac cgcatcgtcg
     1201 ggtattgaga gcttcttccc tagattaatc ttcacctcca cattagtaaa catctgtggt
     1261 ttgagttgtc ctccgggatt ggggagggaa aacctcacct tggcagtcct tgtttcccct
     1321 gatagagtgg gatacacata gtcgattctc gaagagaact ccttcccagg aaaataactg
     1381 agcctgattt gtgccgtttc tcccactttt gttgacggca attcatactc gtagatgtct
     1441 gagattaccc atattgatga gaggtcagcc acgtcaaaga gcttttctcc gggcatcacg
     1501 cgcacgccct gcaacgccat cttttgcacc acatatccac tcacaggact atagatggtc
     1561 aaagtcctta cggccttgcc cgaatcttca attctcctta tctggtcgtc agatatgtcc
     1621 cagagtttca gccgctccct tgctgcctga acaatagcgt cagcgtcctt caaaagcatc
     1681 ttattcatat ccccgctctt ggtctcgctg ccttgcttcg cccacctcag ggcattaagg
     1741 aactcctgct gggttgccac aagctccgga ctatagacct cagcaagtgg ctcgcctttt
     1801 tttacgtact ttcctgtata gtccacatgg agacgctcaa tccagccctc caccttggca
     1861 ttcacagtgg cgagtcttct ctcgtcatat tcaacccttc ccactgtcct tataattttc
     1921 tggagaggcc ttaccgagac ttcggtggtc ctgacaccta tgagccgctg cttttcctgg
     1981 gggatttcca ctgtaggggc ctcttctgtt tctttctgtt ccttctcctg cccgttcatt
     2041 gctgcgggct tggtttggga cggttcgcct cccccctctc ctgagggctg tgctggagat
     2101 tgctgggaaa ctgacggctc cttaccgccc tcctttacgg gaacttttac gaagccagct
     2161 cttgaaagat aaatgacaag tccaaccagg aatatcactg cacctatagt gataagaaat
     2221 cccttcctca tttctcttta tccccgctgc ccgcaatgcc tgtaatcgac tggagtctcg
     2281 caatggcctt ctctctttca gtgaattgcc cccaatacga gatttcgagg tccagaaggg
     2341 tcttcagcct ggtaataacc gttatctcgt ccacccttcc cgtgccgtat cctgagagcg
     2401 cagactcaaa atcctggtat gtcttgggga ttaacccgct tttatacaat tccatcaacc
     2461 tttctgctgt ccgggccatt gagtagttat ccctgatggt tgaagagagc attattttcg
     2521 tggaatccag ttcgtgctca gcccctgata ataaggcccg ggcctcgttt actgcctgtc
     2581 tctgcttcgt tctgtaaaag atagggatat tgatcgtggt agtgaggctc cacatgggat
     2641 caaaatccga ccccctgtta aaatagccgg cattcacagt aaaatcggga tagtagtcga
     2701 gttccgccat cctgaccctg gcttgggctg aggccaccat cctctccttc tctttcacaa
     2761 aaggagaatg ctccacatga agggtgagga gttcatttag acttgtctcc aatccagtcc
     2821 cctttatttc cacaggtctc cccaggggtg cattgatatc cctccctatg gttgagttga
     2881 gcatggcctc taaagactgt atcctctgtt tcagcatctg ctccttttcc aaaagcatgt
     2941 atttttcggt ctgggccatc agcacctcct gctgcatccc ctttcccgac gaatagcggg
     3001 caatggcagc atcttctatt cgcgtgaaga gggccgcccg ttcgttaatg atgtcgattg
     3061 tcttgtaggt gtataagaga tcatagtaga gctcctttac cttctctatg gtccttaacc
     3121 gcactccctc ataggacgcc ctcaaacctt ccgtctccct cgcagccatt tctccctttg
     3181 tggacaattt ccctggaaag ggaaaggtct gggatgctga gaacatccac caggccatga
     3241 tcgattcacc gtaggtatat ttcttccatc cctcattctg atagccgaac atcagcatgg
     3301 gatcgggaag gcttttcact tgcgggattc tgtattctga cgcagccaca cgggattctg
     3361 taataagaat ctcgtgattg tttttcagtg cctcgtcaat aagatcctga agattcagat
     3421 cttcggcaaa gacataagaa aaacacaaca caaggtaagc agtaagcagc agacggactg
     3481 tcctatatgt gggaaggaaa acaaacagag aggaactcct gatcctgtca gggattcccc
     3541 ttgtccgtac gctgcctgcc gcacactt
//
LOCUS       DATKOU010000233        11635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2237963, whole genome shotgun sequence.
ACCESSION   DATKOU010000233 DATKOU010000000
VERSION     DATKOU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237963"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VMU21_11035"
     CDS             <1..2017
                     /locus_tag="VMU21_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HUO78097.1"
                     /translation="PEEGSSPAEPGVNTAEPQPAGAEQQAPPEVKPQGTEQMPSPGAK
                     PEELKQPMPVPRAPIERRKQQPVTQTSQTPSGPPVSFFFDDADVLDVIQTVFADILKV
                     NYVIDQRVKGRVTFRTVTPIPRDEVLSIMKIILRLSGVGFVEEKGLYRILPLTDVPGE
                     LVSAQEGKDPAKVAIELFTFKNLSIKDSMQDIENAIGLSSQSGEIRIIPISRLNAIMV
                     VTATKERMEYVRRWVEAFDTMFAYARPKIMVYPLQNSKAANVSAILQSILSGGTGAAA
                     PGAPGAAPGTAPRATQPGQTPAPGATGTGAAGAPSTPSQPQRAGAAVSVSGSGSGYLI
                     SPDTKIFSDEINNSLIILATPADYAFLEETIKKIDTVQRQVVIEALIVRVDLADNLSF
                     GFSWSFNTDVKISGIQPFKNPINLSGDAQINNPGATVGAGLPTQGFTFVGTDPTGHVR
                     AVITALAHESKAKVLASPHILVSDNREARIQVGQQVPLSTSTTSTPISTGTTTVANTS
                     TSTIQYKDIGIILKVKPQINDSGLVSLELGQEVSSLGDNVVIAGQAFASINKEEVTTN
                     LVALDGETIIIGGLIREDTTKAKDGIPFLSRIPILGNLFSTTTDNVSRNEIIVLLTPH
                     VMKTLVEAEKVTSDYLERYQGTSGKELDEFMNEKGKGSRDGGTKNPR"
     gene            complement(2084..2437)
                     /locus_tag="VMU21_11040"
     CDS             complement(2084..2437)
                     /locus_tag="VMU21_11040"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HUO78098.1"
                     /translation="MSLGMRLVIFILGTSLFLTILELVRKKNFREELSLVWLLIGFGM
                     ILASIADLVIDPLAFSLGIGYPPALVFLIIFFFLVLVVLYFSVVVSDLKGRNKELSQK
                     IALMEYRLNTLRKKD"
     gene            complement(2441..3142)
                     /locus_tag="VMU21_11045"
     CDS             complement(2441..3142)
                     /locus_tag="VMU21_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO78099.1"
                     /translation="MKSYLIVIPAYNEEEHIAGVLDDVKKSNPTADILVVNDGSSDKT
                     SELSRQKGVMVLDIPFNIGYGGAVQTGFRFATEEGYDFVITLDGDAQHDPASVQNLIE
                     AREREGADVIIGSRFLGAPYSMDTARRIGVFIFSRIARLYTGITFTDPTSGFLLLNRT
                     VFSYLSAGDNYPLDYPDVNIIMALHKKKFKVREAPVRMVEKRQGKSMHSGLRPLVYVA
                     RMFLAILMVLLRKED"
     gene            3207..3989
                     /locus_tag="VMU21_11050"
     CDS             3207..3989
                     /locus_tag="VMU21_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO78100.1"
                     /translation="MVNTLEEAKEIFRYRELLRNLVLRDVKIRYKRSALGFLWVMLDP
                     LLMLLVFYVIFAGLFGSVGKYSAYLMSGIIMWQFFSQGTKVASLAFLQNRNLINKVYL
                     PKAIFPLSVVASSLVHFIFSLVPLFIILLSSGTKFGINLALLPFVVGIVFVFSLGMAL
                     TVSTLAVFFHDVVYIYDVVLLGWMYFSAIFYPVSILPQKLQVLMSLNPLYHFISLFRA
                     SLYDPTLLTAEHVLSGSAFALLSFLIGWGIYYGNRDKIIFYL"
     gene            4006..4758
                     /locus_tag="VMU21_11055"
     CDS             4006..4758
                     /locus_tag="VMU21_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO78101.1"
                     /translation="MIALQGVSVKYRLVEERRRSFQAHVINYVKGKRVVKHILWALRD
                     ITLEVGQGESLGIIGPNGAGKSTLLKVISGVMNPSEGMANVRGKTAPLIELGAGFDDE
                     LTGRENIFLNASILGFSRREIEERFDRIVDFSELKEFIDTPLKHYSTGMVARLGFSIG
                     TEANPDILIVDEVLAVGDAHFQKKSRDKILTFKDKGVAILFVSHNMEEVRNLCNQVLW
                     LDHGRVRMLGEPEEVIAEYEKEVAVGGSVRTL"
     gene            4797..5912
                     /locus_tag="VMU21_11060"
     CDS             4797..5912
                     /locus_tag="VMU21_11060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUO78102.1"
                     /translation="MRILHLLYESEGDYFGIGGVGTRAYEIYERLRDRHDITFLCKKY
                     PGAKGGEKRDLKHLFVGVESKNLTKSLLAYAYHAAQYVRRHEDQYDIIIEEFSPAIPT
                     FLHVFTRKPLVLQIQGYTGKLYFRKYNPLYAFTLYTLELLRPRCYDHFIFVKRETATR
                     FRVGSKKHSHIIPNGVAAELLNMPYNEGEYILYLGRIDIYGKGLDILLNAYAQLLEDS
                     PGVRLVIAGDGRDREPFREKIAALPRDVRNNIELLGWVSGDTKNETIRRALFAVFPSR
                     HEVQSIAALEAMASGKAVIVSDIPEFGVVRDAEAGMSFRTGDARSLAQSMKDMVTHNE
                     RGEMGKRGREWVRQFIWDKIALNFERFLNEVAYVSRI"
     gene            5896..6447
                     /locus_tag="VMU21_11065"
     CDS             5896..6447
                     /locus_tag="VMU21_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUO78103.1"
                     /translation="MFREYKPIEWNDEKIKRIWDYTSHFPENYFSYQVGKALIDRLSI
                     FFPNAKNVLDYDCGAGFLIPHLMDKKLEVSALEFSDESLALINSKFRNNRFFKGSYTI
                     EQLKDSAETFDVIMVVEIIEHLNDKYLGQAVHDIKTLPSDNGILIFTTPNDEDLSKSM
                     VSVLNVIMFFTGGNMSGPGIRVV"
     gene            7183..7371
                     /locus_tag="VMU21_11070"
     CDS             7183..7371
                     /locus_tag="VMU21_11070"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HUO78104.1"
                     /translation="MTFPFNLSTITSKISFLNDIVGFLRERKKFWLAPIIIVLLLVSI
                     LMVIGGGSAVAPLVFPLF"
     gene            7580..7870
                     /locus_tag="VMU21_11075"
     CDS             7580..7870
                     /locus_tag="VMU21_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HUO78105.1"
                     /translation="MSPVEVITSVQRRRRWSPEEKRALVEEAEQPGMSISAVARKYGI
                     HPNQLLHWRKLMHIGALSAMRADEEVVPISEVKVLKAKVRELERLLGKKRTR"
     gene            7900..8941
                     /locus_tag="VMU21_11080"
                     /pseudo
     CDS             7900..8941
                     /locus_tag="VMU21_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9066..10172
                     /locus_tag="VMU21_11085"
     CDS             9066..10172
                     /locus_tag="VMU21_11085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUO78106.1"
                     /translation="MKILQLIYESLGNPFGFGGAGVRAYEIYKRLKDRHKVTLLCMKY
                     PGARDGAIEGLRHLFVGTESKSLAKSVFAYTVQAMNFVRHYGDDFDIIVENFLPSTPF
                     FSTLFTRTPVILQVQGIMEKHSFKKFHPFYAIPMHMVERFYPCLYKRFIFVSPVTKEK
                     IMRRLGRKNVQCQVIPNGINDDLLRVTPEDGGYILFFSRIDIYTKGLDILVEAFKKIA
                     SEYPEIRLILAGYEFDRFETLVSGSHLSLKERIEYAGFVAGEEKVRLLSGATCVVLPS
                     RHESSPVSVLEAAACGKAVIVSDIPEMSFVEEKGFGLSFPCCSADRLSEKMRIVLGDG
                     KKRAVMGTRGREFAAQFLWDHIAIDFENVLNHSA"
     gene            10273..11052
                     /locus_tag="VMU21_11090"
     CDS             10273..11052
                     /locus_tag="VMU21_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HUO78107.1"
                     /translation="MLSESEIIPYHLSPPAGERVVVLAPHPDDETFGCGGALSLLVDA
                     KKKIKVLFLTSGDKAESRGKGFSDREHITEYSLLREREAERALGVLGVSEYEFLRFPD
                     REIHEHYQDALQSISKTVEEFSPDTIYAPSLVELNPDHRTAAAMSMEIQRRFMAGPAE
                     CDGRSLVRIVFYEVATPLRPNTLVDITPVYRRKKRAMKRYRSQLKLADYLGHTAALNT
                     VRALTVKGPRYVEAFWCIETPLSSEEIANWLSYREVLAAGY"
     gene            complement(11445..>11635)
                     /locus_tag="VMU21_11095"
     CDS             complement(11445..>11635)
                     /locus_tag="VMU21_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78108.1"
                     /translation="IFTFNLLLIKLFSSLFNLSQPMAENLSYKKTEPQIHKSSLPLKN
                     LSPPFPWFIDKNRAINDS"
BASE COUNT         3138 a         2535 c         3030 g         2932 t
ORIGIN      
        1 accagaggaa ggcagttccc cggctgaacc gggggtaaat accgctgaac cccagccggc
       61 cggagcagag caacaagcgc cccctgaagt taagccgcag gggacagaac aaatgccctc
      121 ccccggagcc aaaccggagg agttgaaaca gccaatgcct gtgccgagag cgccgattga
      181 gcgtaggaaa cagcagccag tgacacagac gtcccaaacg ccctcagggc ctccggtgag
      241 cttctttttc gacgatgcgg atgtcctcga cgtgatccag accgtcttcg cggatatcct
      301 caaggtgaat tatgtcatag accagagggt gaagggcagg gtgacgttca gaaccgtcac
      361 ccccattccg cgtgacgaag ttctttccat aatgaagata atcctgaggc tcagcggcgt
      421 gggctttgtg gaagagaagg ggctttacag aatacttccc ctcactgatg tgcccggcga
      481 actggtgtct gcccaggagg gaaaggaccc ggcgaaagtg gcaatcgagc tcttcacgtt
      541 taagaatctc agcataaagg attctatgca ggacattgaa aatgccatag gattgtcctc
      601 gcaaagcgga gaaatcagaa tcattcccat atcccggctg aatgccatca tggtggtgac
      661 agccacaaag gagcggatgg agtatgtgcg gagatgggtc gaggcctttg atacgatgtt
      721 tgcctacgca cggcccaaga ttatggtcta ccctctgcaa aacagcaagg cagccaatgt
      781 ctccgccata ctccagtcga tactctcagg gggaacaggg gccgctgctc ccggtgctcc
      841 gggtgcggca cccggcacgg ctccaagggc aacccagcca ggacagaccc ccgctcccgg
      901 tgctacgggt acgggcgctg ccggtgctcc ttcgacccct tcccagcccc aaagagcggg
      961 cgcggcagta tcagtatcgg ggtcgggatc gggatacctg atctcaccgg acacaaaaat
     1021 tttttcggat gaaatcaaca attccctgat cattctcgca actcctgctg attacgcctt
     1081 tctcgaagag acgataaaga agatcgacac tgtgcagagg caggtggtga tcgaggcgct
     1141 cattgtgagg gtggatctgg cagataacct gagctttggt ttttcctggt cgtttaatac
     1201 tgacgtgaag atttcgggaa tacagccttt taagaatccc atcaatctca gcggtgatgc
     1261 ccaaatcaat aacccggggg cgacggtggg ggcgggcctc cctacccagg ggtttacctt
     1321 tgtcggtaca gacccgacag gacatgtgag ggcagtgatc accgccctag cccatgagtc
     1381 aaaggcaaag gttcttgcct cgccccacat ccttgtttcg gataaccgtg aagcgaggat
     1441 acaggtgggg caacaggttc ccctctcgac ctcaacaacg agtactccta tatccaccgg
     1501 aacaaccact gttgcgaaca caagcacttc tacgattcaa tataaagaca taggaatcat
     1561 ccttaaggtg aaaccccaga tcaatgacag cggacttgtt tccctcgaac tgggtcagga
     1621 ggtctcctct ctcggagata acgtagtcat tgccggccag gcatttgcat ccataaataa
     1681 ggaagaggtc accaccaatc tcgttgcgct ggacggggaa acaattatta tcggggggct
     1741 cataagggaa gataccacta aggcaaagga cggaatccca tttctgagca ggatccctat
     1801 cctcggcaac ctcttcagca ctaccactga caatgtctcg agaaatgaga tcattgtcct
     1861 tctcacgccc catgtcatga aaactctcgt ggaggcggag aaagtgactt cagattatct
     1921 tgagcggtat cagggcacaa gtggcaagga acttgatgaa tttatgaacg agaaaggtaa
     1981 gggtagccgt gatgggggga ccaaaaatcc caggtagccc ggtgtctttc cctgtgagtg
     2041 gttcaagaat ccttccctct gcttattatc tactgaaacc atatcaatct tttttccgca
     2101 aggtattcag cctgtattcc atgagcgcta tcttctgact caactctttg ttcctgcctt
     2161 tcaggtcgga tacgacaaca gagaagtaca atactacgag aaccaaaaag aagaatatga
     2221 tcaggaagac caatgcgggg ggataaccga tccccagact gaaggcaagc ggatcaatta
     2281 caaggtccgc aatggaagcc agaatcatcc cgaatcctat taacagccag acgagagaga
     2341 gctcttcccg gaaatttttc tttctcacca gttccaggat cgtgagaaac agggaagtgc
     2401 ctaaaataaa aatcactaag cgcataccaa gggacatacg ctaatcctcc tttctgagca
     2461 gcaccatgag aatggccaga aacatcctcg ccacgtacac aagaggcctc aacccggagt
     2521 gcatcgattt gccctgacgc ttttccacca tcctcaccgg ggcctccctc accttgaatt
     2581 tcttcttatg gagcgccatg ataatgttca catcaggata gtccagggga tagttgtcac
     2641 ccgcggaaag ataggaaaac accgttctgt taagcagcag aaatcccgag gtaggatcgg
     2701 taaaggttat tccggtataa agccgggcga ttctggagaa gataaacacc ccaatccttc
     2761 tcgcggtgtc catggaatag ggtgccccca aaaagcggga gccgatgatc acatcagcac
     2821 cctcgcgttc ccttgcctct atgagattct gcaccgaagc agggtcatgt tgtgcgtcgc
     2881 catccagggt gatgacaaag tcataacctt cctctgtcgc gaagcgaaat cctgtctgga
     2941 ccgccccacc ataaccgata ttaaaaggga tatccagaac cataacgcct ttctgtcttg
     3001 acaattcaga tgttttgtca gaagagccgt cgtttacgac caagatgtcg gctgtgggat
     3061 tacttttctt gacatcatcc aggacaccgg cgatatgttc ttcttcatta tacgcaggga
     3121 taacgatcag ataagacttc aagattccac ctatttttca agaatacgcg gcattacgat
     3181 gtgttattat agcaggggag aggactatgg taaatacctt ggaagaggca aaagagattt
     3241 tcagataccg ggaacttctc cgtaatcttg tcctgaggga tgtcaagata cgatacaagc
     3301 gttccgctct cggctttcta tgggtcatgc ttgacccatt gctgatgctg cttgtctttt
     3361 atgtgatatt tgccggactt ttcgggtccg tcgggaaata ctcagcctac ctcatgtcgg
     3421 ggatcatcat gtggcagttt ttttcgcagg ggacaaaagt cgcctcgctg gcttttcttc
     3481 agaaccgcaa cctcataaac aaggtatatc tgccgaaagc tatcttcccc ctctcggtcg
     3541 tagcctcttc gctggtgcat tttatctttt cacttgttcc cctcttcatt atcctcctgt
     3601 cttccggcac aaagttcggt attaaccttg cgttgctgcc ctttgttgtg ggtattgtct
     3661 ttgtattttc tctcggcatg gccctgactg tctcgacgct cgcagttttt tttcacgatg
     3721 tggtctatat ctatgatgtc gtgctgctgg gctggatgta cttttcagcg atattttatc
     3781 cggtctccat tcttccccaa aagcttcaag tgcttatgtc gcttaaccct ctctatcact
     3841 tcattagtct tttcagggca agtctttatg accccaccct tctgacggca gagcatgtcc
     3901 tttcagggtc ggcctttgcc ttactctcct ttttgatcgg ttggggcatt tattacggga
     3961 acagagacaa aataatattt tacctttgag aaaggaagca tttccatgat agcgcttcag
     4021 ggcgtatcgg tcaaatatag acttgttgag gaacgcagga gatcattcca ggcgcacgtt
     4081 atcaattatg taaaggggaa gagggtagta aaacatatcc tgtgggcgct gagggatata
     4141 acattggaag tcggtcaagg ggaatcgtta ggtattatcg ggcccaatgg cgcggggaaa
     4201 agcactttgt tgaaggttat ctccggggtt atgaatccct ccgaaggaat ggccaatgtg
     4261 aggggcaaga ctgcccccct gatcgagctc ggcgcaggtt ttgatgacga gctcacggga
     4321 agagaaaata tcttcttgaa tgcttcgatc ctcggttttt cgaggaggga gatagaggag
     4381 cgctttgaca ggatcgtgga tttttcagaa ttgaaggagt ttatcgatac accgctgaag
     4441 cattattcca ccgggatggt ggcgcgcctc gggttttcca ttggcacgga agcgaatccc
     4501 gatatcctga ttgtggatga ggtactcgca gtcggagacg ctcactttca gaaaaaaagc
     4561 cgagacaaga tactgacttt caaagacaag ggggttgcta tactgtttgt ctcacacaat
     4621 atggaagagg tcaggaacct ctgcaatcag gttttgtggc ttgatcacgg gagagtaagg
     4681 atgctgggag agccggaaga ggtgattgcc gaatatgaaa aagaggtagc ggttggcggc
     4741 agcgtcagga cgctctgaag aagcggccgg gaaggacaat atacctttga ggcatcatgc
     4801 gaattcttca ccttttgtat gaaagcgaag gagattactt cggaatcggc ggagtaggaa
     4861 caagggcgta cgagatttat gaacgactaa gagatcgcca cgatattaca ttcttatgca
     4921 agaagtaccc gggagcaaag gggggagaaa agagagatct gaagcaccta ttcgtaggag
     4981 tggagagcaa aaacctcacg aagagcttgt tggcgtacgc ttatcatgcc gcacaatatg
     5041 tgaggaggca tgaagatcaa tatgacatca tcattgagga attctcacct gctattccca
     5101 cattcttaca tgtcttcaca aggaaaccgc tggtattgca gatacaggga tatacaggga
     5161 aactgtattt cagaaaatat aatccccttt acgcatttac cctttatacc ctggagctgc
     5221 tgagaccccg gtgttatgac cattttattt ttgtgaaacg ggaaactgcc acgagattcc
     5281 gggtgggaag caaaaaacac tcccacatca ttcccaatgg tgtggcggct gaattgctga
     5341 atatgccata caatgagggt gaatacattc tctatcttgg gaggatagat atctacggga
     5401 aaggacttga catcctcctg aatgcatacg cgcaattgct tgaagactcc ccgggggtga
     5461 gattggtcat tgcaggggac ggaagggaca gagaaccctt cagggagaaa attgccgcgt
     5521 tgccacgaga cgtcagaaac aacatagagc tgctgggctg ggtttccgga gatacgaaaa
     5581 atgagacaat aaggagggcg ttatttgcgg tcttcccttc aaggcacgag gttcagtcaa
     5641 ttgcagccct tgaggcaatg gcatctggca aggcggtcat tgtgagcgat atacccgaat
     5701 tcggcgttgt gagagacgcg gaggcgggaa tgtcttttag aacaggggac gcgcgttccc
     5761 tggctcagtc catgaaagat atggtgacac acaatgaacg gggggagatg ggaaagaggg
     5821 ggagggaatg ggtaaggcag tttatctggg acaagatcgc cctgaatttc gaacggtttc
     5881 tcaatgaggt tgcttatgtt tcgagaatat aaaccaattg aatggaatga cgaaaaaata
     5941 aaaagaatat gggattatac gtctcacttt cctgaaaact atttttctta tcaggtcggc
     6001 aaagctttaa tcgatcgctt aagtatattt ttcccaaatg ctaagaatgt gctcgattat
     6061 gattgtggtg ccggtttttt gatccctcat ttaatggata agaagttaga ggtttccgct
     6121 ctggagttct cagatgaatc attggcgctc ataaatagca agttccggaa caatcgtttc
     6181 ttcaagggtt catatacaat agagcaactg aaggattctg ccgaaacatt tgatgtcatt
     6241 atggttgttg aaattattga acatttaaac gataaatatt tgggtcaagc tgtccatgat
     6301 attaaaacgt taccttctga taacggcata ctcatattta ccacccccaa tgatgaagac
     6361 ttatccaagt caatggtatc tgtcctgaat gtgatcatgt ttttcaccgg tggcaacatg
     6421 tcaggacctg gaataagagt agtttaacac tctttttaca atcgaatgga tttgatattg
     6481 cggctatatt taaaacaaat ttttcgaaaa cgaaattact aataggactt gaatcgcttt
     6541 taagcagatt attacagcgc taaatcccta taagttttta attgaggcac atttacaaag
     6601 gctctgtata gatcttcttc agtcggctgc tctttatttt taagaatata gtttgtatct
     6661 gcagacagcc gttgcaaaaa atcgtttacc cccgccaaat atataacagt tttgatatcc
     6721 tgatactgtt cgagcaaata tttaggctgt aaataatttt caacagatgt gtgaccgctt
     6781 ctcccaacat ttccaaccca gtaggaatga gtttttccat tatcattcag taatgcttgt
     6841 agtaaataag gccaggtctt tttttggtca agatataaac actctgttgt gctgccgcca
     6901 attgctcaaa tcttgttttc acattgcttg ggaatttcat cgcctcttat tccatctgaa
     6961 ttagtcagga agtttgaatc tccatatacg ccgggaagga tgccgctttt tatcttaaga
     7021 attctcttaa gattcggttg ccaggcaaaa tatctatttc tgtctttatt aattgccctt
     7081 aaagcgattt cagaaacaag agaggcaaca attatctcta ttgttacaga ggcggcaata
     7141 agctgaattg tttaagattt ttcattcatt aggagaattc aagtgacttt tccatttaat
     7201 ttatctacaa taacatcaaa gataagcttt ttaaatgata ttgttggatt tttaagagaa
     7261 cggaagaaat tctggcttgc tccaataatt attgtccttc ttttagtaag tattctgatg
     7321 gttattggcg gagggtcggc agtagctcca cttgtgttcc ccctatttta aatgcaggca
     7381 aagagcaaag gagaaaagct taaagcccaa cttgttatcg taacaggttt tttgtttctc
     7441 tattttgttt ttaagaaatc tctttttctt tattcttcct taatgaagta gcccccgttt
     7501 agaccggaca caggtacgaa ccatgataag ttgtagggat agatgtagaa ttatacctac
     7561 aaccaagaca aggaggcgta tgagtccggt agaagtcatc acatcagtcc agaggcgtag
     7621 gcgctggagc cctgaggaaa agagagcatt ggttgaggaa gcagagcagc ccggcatgag
     7681 catttcagct gtagcccgga aatacgggat acatcccaac cagctgttgc actggaggaa
     7741 gctcatgcat attggagcgc tttcagcaat gcgggcagac gaagaggttg tccctatctc
     7801 cgaggtcaag gtgttgaaag cgaaggttcg ggagttagag cgtctcttgg ggaaaaagag
     7861 gaccagataa tcggggtttt gaaggaggcc ggagctggta tgcctatagc cgagctatgc
     7921 cgcaagcatg ggataagcga tgcaacctac tacaactgga aggcgaagta cggcggcatg
     7981 acggtcagcg aggcaaagaa gctgaagcag ctggaggatg agaaccgtcg gctgaagcag
     8041 atcgtagcgg atctcagcct tgacaatcag gcactgaaag cggtcatctc aaaaaacttc
     8101 taagacccaa ggcgcggaaa gaagcagcgg tatttgtcat gggaggcttt gggctgagtc
     8161 agcgacgggc cagtagtctt gtcgggttgt gccggaacac tctacggtat caggcaatgc
     8221 ccagcaatga cgaagaactc cgcaagaggc tcagggagct gagcgagaag aagagacggt
     8281 atggatgtga aagactacat ctgtttctca ggcgagaggg tttggtgaag aatcataaga
     8341 ggacagagcg catatatcgt gaagaagggc ttacgttaag gattcgaagg aggaagaaac
     8401 tggatcccag ggaaggattg agttatcgaa ggcctgccgt accaatgagc aatgggcgat
     8461 ggactttgtt tctgatgcat tgagtagcgg caggaggata agactgctgc cgatcattga
     8521 tatgtatacg agggaatgtt tgaggatcga ggtggatacc tccattggcg ggcagagggt
     8581 cgcaacaatt ctcagtcaga taggggctat gaggggatta cccgatcata tcatggttga
     8641 caatggcccg gagttcatca gtaatgcact ggatgcatgg gcttatgagc gtgggattaa
     8701 gctgcagttt attcgcccgg ggaagccggt tgataacgcg tacatggaga gtttcaacgg
     8761 caagttaagg gatgaatgcc tgaatcagaa ctggtttatg agtcttgatc atgctcgtcg
     8821 ggttattgaa gaatggcgta ttgaatataa ccaggagcgg cctcacagtt ccctgggtaa
     8881 tctgacacca aatgaattca ggctggagca agaaaatgta ctggaattct ctaatccata
     8941 gtggcacaat gaatggggat aggtcccttg agtggacgta tgaaatttag tcttcccgat
     9001 gggggggagc acctgctcat cacccgaatg acggggcccc ccgatggtcg acgaaggggc
     9061 gattcatgaa gatcctccag ctcatctatg aaagtctcgg gaacccattc ggattcgggg
     9121 gggccggggt gagggcctat gagatttata aaaggctgaa ggacaggcat aaagttactc
     9181 ttctctgtat gaagtatccc ggggcgaggg acggcgctat tgaagggttg aggcatctct
     9241 ttgtgggaac agagagcaag agcctcgcaa agagcgtttt tgcctatacc gttcaggcga
     9301 tgaactttgt aaggcattat ggcgatgatt tcgatatcat cgtagaaaac tttttacctt
     9361 caacaccttt tttctcaaca ttgttcaccc gtacgcccgt tattttgcag gttcagggca
     9421 taatggaaaa gcattctttc aagaagtttc atcccttcta tgcgattcca atgcatatgg
     9481 ttgagcggtt ctacccgtgt ctttataagc gcttcatctt cgtgtcaccc gtcacaaagg
     9541 agaagataat gaggaggctc ggaaggaaga acgtgcaatg tcaagtaata cccaacggga
     9601 tcaacgacga ccttctgagg gtaacccctg aggacggtgg gtatatcctc tttttcagca
     9661 gaatagatat ctatacaaag ggtctcgaca ttctcgttga ggcattcaaa aagatcgcct
     9721 ccgagtatcc tgaaataagg ctgatccttg ccgggtatga attcgacaga ttcgagaccc
     9781 tggtctcggg ctcccatctt tctctcaagg agaggatcga atatgcgggg tttgtggcgg
     9841 gagaagaaaa agtgaggctc ctgtccggag caacgtgcgt ggtcctgcca tcacgccatg
     9901 aatcctcgcc ggtgagcgtc ctcgaggctg ctgcatgcgg gaaggcggtg atcgtgagcg
     9961 acatcccgga aatgagtttt gtggaagaaa aggggtttgg gctcagtttt ccttgctgtt
    10021 cagcggaccg gctgtcagaa aaaatgagga tagtactggg tgatgggaaa aagagggcag
    10081 tgatggggac acggggcagg gagttcgccg ctcaattttt gtgggaccat attgccatag
    10141 attttgagaa tgtgttaaac catagtgcat aacttttgcg tatgactgat tcaaggcgaa
    10201 tatggacaaa aagcgtatca taaagatggt cctggcgaag acgaggggga aatcggctca
    10261 gggaggcggg tgatgcttag tgaatctgaa ataattccgt accatctctc gcctcccgca
    10321 ggtgaaaggg ttgtggttct cgcccctcat cctgatgatg aaaccttcgg atgtgggggg
    10381 gcactcagcc ttctcgtcga cgcaaaaaag aaaatcaagg ttctctttct caccagcggg
    10441 gataaagcag aatccagggg gaagggattt tcggacagag agcatattac ggaatactcc
    10501 ctgctgaggg aaagagaggc agaaagggcc ctcggggtcc tgggcgtttc tgagtatgaa
    10561 ttcttacgat tccctgacag ggaaatccat gagcattacc aagatgcgct tcagagcatt
    10621 tccaaaacag ttgaggaatt cagtcctgat acaatctatg caccgtcact ggttgaactc
    10681 aatccggacc accgcactgc cgctgccatg tcaatggaga tccagaggag atttatggca
    10741 ggtcctgccg agtgtgacgg aaggtcactg gtacgaatcg ttttctacga ggttgccacc
    10801 cctttaagac ccaataccct tgttgatatc acccccgtat atagaaggaa gaagagggcc
    10861 atgaagagat atcgttcaca gctgaagctt gcggattatc tagggcacac cgctgcatta
    10921 aacaccgtca gggcattgac ggtgaaaggg ccgcgatacg tggaggcatt ctggtgcatc
    10981 gaaacgccat tgagcagtga agagatagcg aactggctga gttacagaga agtgctcgct
    11041 gccgggtact aggcggaccc tcaggaagga cacatgccca agggaatgaa tggtttcgat
    11101 gcgggaatta ctaatctcct ctggggtgta ggggtgagaa tatacatatg ctgcataagc
    11161 tgcacacaca gggcgttgaa cctcgcatga actcaaagat tttgaagaag tcgtatttgg
    11221 cttgtttttt gcttaataag tcactcacat ttctttcttc aaaaacactt gacaagaggg
    11281 gattctctgt ttatacttct tacagactag tttacggact aggattttgc aggccagagc
    11341 gtgggtatgg acctaaaaaa aagcttgaca aggacagtgc ctttcattac aattgaggca
    11401 aatgattagg gcgagggggt gatgagtttt ccaaaaaata atcatcagct atcgttgata
    11461 gccctgtttt tatcaataaa ccatggaaaa ggaggtgata gattttttaa aggaagggag
    11521 gatttgtgaa tttggggttc tgtttttttg tagctgaggt tctcagccat cggctgagag
    11581 agattaaaga gactggagaa tagtttaatc agaaggaggt tgaaagtgaa gataa
//
LOCUS       DATKOU010000234        20196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4181944, whole genome shotgun sequence.
ACCESSION   DATKOU010000234 DATKOU010000000
VERSION     DATKOU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 20196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20196
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4181944"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(584..1081)
                     /locus_tag="VMU21_11100"
     CDS             complement(584..1081)
                     /locus_tag="VMU21_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78109.1"
                     /translation="MSSDPMSDIVMATIRCVMAVIANRSYLKFLALSGYVLFCAMIFM
                     TQPIGAQARDISNINASPDEVMSRLKTRLSLTEEQEAKMRPIIEGSIEKRREIVKNSS
                     DDRKAVKSQLQELKWSTDMQIGKILTEEQMKEYQKLREEQTEKAENNETHSGRKSRGG
                     GSRGF"
     gene            complement(1530..1655)
                     /locus_tag="VMU21_11105"
     CDS             complement(1530..1655)
                     /locus_tag="VMU21_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78110.1"
                     /translation="MKIAPCVKIPCGLAAGFFILTDILKAFIHTVLEYGSDAFIS"
     gene            1748..2953
                     /locus_tag="VMU21_11110"
     CDS             1748..2953
                     /locus_tag="VMU21_11110"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HUO78111.1"
                     /translation="MATGELREDEREPKPHPAASRKHWWIWLTAVCLIAVGLYFYFAQ
                     APAGSAKQGPNVRTVPVVTAAARKGDIRIYLNGLGSVTPLNVVTVKSRVDGQLMKVLF
                     REGQVVSAGDLLAEIDPRPFEVQLTQAEGQMARDQSLLKNAVLDLERYKELSQRDLIP
                     KQQLDTQEALVRQYEGVLKLDQGQIDNAKLQLIYCHITAPISGRVGLRLVDAGNIIHA
                     SDPNGLVVIAQLQPITIIFSIPEDNLTELLRKLKAAKGIPVEAFDREQNQKIASGYLL
                     TVDNQIDPTTGTVRLKAMFPNKENELFPNQFVNARLLLDTKHGTTIVPQAAIQRSPKG
                     AFVYVVKADKTAEVRPVRAGPSEGDDISIDEGLSPGEIVVVEGADRLREGSKVELQGQ
                     AQNNSRKGG"
     gene            2954..6079
                     /locus_tag="VMU21_11115"
     CDS             2954..6079
                     /locus_tag="VMU21_11115"
                     /inference="COORDINATES: protein motif:HMM:NF007798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HUO78112.1"
                     /translation="MNPSRSFIVRPVATTLLMAAILLAGAVAYRLLPVSALPQVDYPT
                     IQVVTFYPGASPDVTASSITAPLERQFGQMPGLRQMTSTSSGGSSVITLQFSLDLSLD
                     VAEQEVQAAINAATTFLPRDLPNPPVYSKVNPADAPILTLALTSNTLPLPQVEDLADT
                     RLAQKISQLSGVGLVSISGGQRPAVRVQANPTVLAAYGLTLEDMRTALAAANVNQAKG
                     GFDGPRQSSIIGANDQLLTSKEYRPLVVAYRNNAPVLLSDVATVIDDAENVNQAAWMN
                     DAPAVIVNIQRQPGTNVIEVVDRVKRLLPQLQSSLPSTVQVSILTDRTTTIRASVKDV
                     QFELMLAVVLVVMVIFLFLRTLAATSVPSVAVPLSLVGTFGIMYLVGFSLNNLSLMAL
                     TIATGFVVDDAIVMIENIARYIEQGEPPLQAALKGSEQIAFTIMSLTVSLVAVLIPLL
                     FMGDVVGRLFREFAITLGVTIVISAVVSLTLTPMMCARLLKHKPEEQQKRLFRVSQQL
                     FDRTIELYGKTLKWVLQHQTPTLLVAVGTLILTVFLYMVVPKGFFPVQDTGLILGISE
                     APQWTSFTAMAEQQQALSRVILKDPAVKSLSTFIGVDGTNTTLNSGRVLINLMPIEER
                     KMSASDIIRRMQPELAKLRGITLYMQPVQDLTVESRVSRTQYQYSLEDPDINELNSWT
                     PRVVDALSRLPELRDVSSDQQDQGLRASLLIDRTTASRLGITPQLIDDTLYDAFGQRQ
                     VSTMFTQLNQYRLILEVRPEFKQNPGKLKDIYIRSSGGGQVPLGAITRTAETTGPLVI
                     SHQGQFPATTISFNLAPGESLGKAIKEIDSITQQLGLPASIRRSFQGTAQAFQSSLAN
                     EPLLILAALITVYIVLGVLYESYIHPITILSTLPSAGVGAILALLICRTEFSVIALIG
                     IILLIGIVKKNGIMMVDFALDAERREGKSPVDAIYQACLLRFRPIMMTTLSALLAALP
                     LALGSGMGAELRRPLGISIIGGLIFSQMLTLYTTPVIYLWFDRLARRLGRVRSENSDD
                     VPDDRAGGTGS"
     gene            6079..9204
                     /locus_tag="VMU21_11120"
     CDS             6079..9204
                     /locus_tag="VMU21_11120"
                     /inference="COORDINATES: protein motif:HMM:NF033617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HUO78113.1"
                     /translation="MNISAPFIKRPVATTLLTVGMVLVGIVAVRLLPVAPLPQVDFPT
                     ISVSAALPGASPETMATSVAAPLERQFAHIAGVTEMTSTSTLGATSITLQFELSRNID
                     GAARDVQAAINAARSYLPAALPSNPTYRKVNPADAPILIIALTSDTVGRGRMYDVAST
                     ILQQKLSQAEGVGQVIVGGGSLPAVRVELNPTALNKYGFGLDDVRTALNSTNANRPKG
                     QLADGTRTWEITTNDQMYKAEEYKPLIVAYRSGAAVRLSDVADVQDSVEDIRNAGLVN
                     GQPAVPVILFRQPGANIIETVDRIKDMLPQLEASIPGAVRLSVVMDRTTTIRASLKHV
                     EHTLVIAGVLVIMVVFAFLRNIRATLIPAVAVPVSLISTFGVMYLFGYSIDNLSLMAL
                     TIATGFVVDDAIVVLENITRHLEKGVPARQAAYLGAREIGFTVLSISISLVAVFIPIL
                     LMGGMLGRLFREFAVTLSAAILVSLVVSLTATPMMCAALLKPETARSHGRLYKAGERV
                     FDWMHSHYEETLSWALRHPRFMLALTLITIVTNIFLFVVVPKGFFPQQDTGRLTGSIQ
                     AAQDISFQAMKEKLTAIVNIIKDDPAVDNVIAFTGGGGGGVTRNTARMFIALKPQGER
                     KLSADQVIARLRKKLVSVPGAPAFLQAVQDVRVGGRISAAQYQYTLQGDDLNELMAWA
                     PRMEQQLRALPGVVDVNSDQQDKGMQSTVVVDRSTASRLGITSQVIDDTLYSAFGQRQ
                     VSIMYTLLNQYHVVMEVEPRFWQRPESLRNIYVHSSNGALVPLSTFSRYEPTATALAV
                     GHQGQFPSVTLSFNLAPGAALGDAVKKIETTTRKMGLPASIRGSFQGTAQVFQSSLAS
                     EPLLILAALVSVYIVLGILYESYIHPITILSTLPSAGVGAIFALLISRTELSVIAIIG
                     IILLIGIVKKNGILMVDFALDAERREGKSSVDAIYQACLLRFRPIMMTTMSALFGAVP
                     LAVGFGVGAELRRPLGIAIVGGLIFSQMLTLYTTPVVYLYLDRFRLWLENKRKKMGWV
                     RRGIVSGPGEM"
     gene            complement(9323..10789)
                     /locus_tag="VMU21_11125"
     CDS             complement(9323..10789)
                     /locus_tag="VMU21_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HUO78114.1"
                     /translation="MELSGKTKIDELLKDYPFLMEFFINRSPKFKMLQSTVMRKTVGK
                     VASLSHVAHIGGIELPLLLKEIAATIKEKTGQDVPIVEESTEAGPIQDAEMRQEVLKD
                     IIRDLHKGVDMAVLKQRFYELIKDIDATEIARMEQKLMEEGMPETEIKRLCDVHVEVF
                     KEALGAQEAPSAPPGHPLHTFMKENRAVEGIVHEIKGIMDNLCDTSDKEVFRQHRKNL
                     GALIERLSDINLHYLRKENQLFPVLEKHGISGPSEVMWAIHDDIRQTLKYARAQISEL
                     QVPLVYGTLKYLLQSINDMIYKEEHILFPMAMEMLAEADWIKVRQGEEEIGYAWVEPE
                     KGWMPGAEVPAEARAAVESGAVDLSTGRLTREQVDLLLTHLPVDISYVDEKDELVYYS
                     ATKERIFPRSPGVIGRKVQKCHPPKSFHVVQKILDEFRAGKRDVAEFWIQMKGRFIHI
                     RYFAVRDSGGKYRGTLEVSQDVTKIRKLEGKQRLLDWD"
     gene            complement(10912..12222)
                     /locus_tag="VMU21_11130"
     CDS             complement(10912..12222)
                     /locus_tag="VMU21_11130"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HUO78115.1"
                     /translation="MDNLVSFTGIFVLMFVAWTMSAHRKVINWRVIVWGLTLQLVFAF
                     FIFVVPVGSRLFLVINDAVVKVLDSAGAGTRFLFGRLALPPGTKNEAGEESLGFFLAF
                     QALPTIVFFSSLMMLLYYIGVMPRLIKLFSTTFTKLMRISGAESLSVSSNIFVGVEAN
                     MTILPFLGGMTRSELCTILSAGMATTASNVLALYVMLLQGRFPTIAGHLISSSILSAI
                     GAIVISKIIMPETETPETLGLEIAPHYQKESTVMEAIINGANEGMKMVVGIMALLLAF
                     LGIVALVDSLLGGLGGRLNNFAGLQMDWSLKGLLGYLFYPLTLAIGVPPSDAFEISKL
                     IGERAVLTEVKSYQDLAVLLSTGGLKNPRSAVLAAYALNGFAHFASLAVFVGGTAALV
                     PKRTKDISAIGFRALIAATLACLMTAAVAGTFFTQGSILLGAPH"
     gene            complement(12255..13034)
                     /locus_tag="VMU21_11135"
     CDS             complement(12255..13034)
                     /locus_tag="VMU21_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO78116.1"
                     /translation="MAEGNAIYVLVAREFKKFIREKSRLVSTLARPLIWLFLVGGGMS
                     RLVSPGMGIVSPGMGISYIQFIFPGILGMTILFSAIFSSISIIWDKEFGLMKEILVAP
                     VSRLSIVLGKALSGTIISVIQAAIILALFPFLGIRLGVLPIIEALVICTLLSFCIASL
                     GIVIATFYESFESFSVIMNFIVMPMFFLSGAMYPVKMLPSILKLLTRINPFTYGIDAL
                     KHAIFREQGVFDFSLFMDIGVLVATSAVFVTISGLLFEKRK"
     gene            complement(13021..14007)
                     /locus_tag="VMU21_11140"
     CDS             complement(13021..14007)
                     /locus_tag="VMU21_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO78117.1"
                     /translation="MPIIRVEHLVKRFGNLTAVNDISFEVEEGTIFGFLGPNGAGKTT
                     TINILCTLLSPTSGKASIYGHDCTTKSPEVRKSIGIVFQDSTLDKDLTAYENLVFHAY
                     LYNVDKDERKQRIEDALRFVDLYDRRNDLVKKFSGGMKRRLEVARGLIHRPKVLFLDE
                     PTLGLDPQTRANLWEFIVALPKEHQVTIFMTTHYMEEAEVCDQIAIIDKGKIIALDTP
                     EKLKKTVGGDVIYIRTTDNLTARREIEKLFHVEVSEQDGELFLTTLKGDACIPEIIRA
                     MGETVVSVRLQRPTLNDVFLKMTGKEIRAEGVSSGDTIKEEVRSYRRRFGRG"
     gene            14035..14232
                     /locus_tag="VMU21_11145"
     CDS             14035..14232
                     /locus_tag="VMU21_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78118.1"
                     /translation="MENDDYYIIEGRMAWPLTWVVILGTLPGVFIAYFIRVLYLPDPG
                     TFRLFVGCVPLFIGGADCFMN"
     gene            complement(14340..16307)
                     /locus_tag="VMU21_11150"
     CDS             complement(14340..16307)
                     /locus_tag="VMU21_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78119.1"
                     /translation="MTEKLRLSLASKLLITSVLIIVGITILFWLPSIVHIQKEELNKN
                     INFAESQTDVIQRALHYGMLTNNREFIKQTIESLASMDNILWIRITDPEGGVHFSSVR
                     GETGARPDSAISNVRDEEGRTSMMKKVRGQRVLEIIQPIHNMHPCYTAKCHAHSKDQK
                     ILGRIETEYSLRSVDAYISKQRYTLAAFGFVFIAVLFVPLYLVVQHFVLSPVVLLKEG
                     IQRVTAGDLSHPIEIDAKDELGMLARSFNSMTKNLQEKSNAVTKELDEYRTSLLHAQK
                     MEAIGTLSAGIAHDFNNILTGILGYSELALDETTHQPVKEHLQRVLELTQNATEFTKQ
                     ILLIGRKLPPARQPTDINRLIEESMRMLRRMVEENIDIRLIPRTGLHMVNADQSQITQ
                     VLMNLVVNARDAMPKGGAIEIRTGESHVDEEYCTYYAYAAQGYFVTITVSDTGEGISE
                     EIKTRIFEPFFTTKVKGKGTGLGLAVSYSIIRAHGGWINFYTEEGKGTAFTIYLPAYG
                     PGPDTVVKSRDISQKQALPRGKECILLVDDEEVIRDLGKSILHGLGYEVITAANGEEA
                     LRIYIKRHDEIALVIMDRVMPKIDGIESYKLLKEVNPRVRVIISSGYAADEALNLKEL
                     GVMGFLDKPYRMAEMANMVRRSIDGGEGESS"
     gene            16607..17506
                     /locus_tag="VMU21_11155"
     CDS             16607..17506
                     /locus_tag="VMU21_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HUO78120.1"
                     /translation="MSLSGEKRLGLSLAVTAAILIAEVAGGLLSNSLALLSDAGHVLT
                     DSFALGLSMVAARISRKPSDYRATYGYQRVGLLAAVINGLSLVAISAFIFFEAYQRIT
                     SPPSINVPLMLLIATLGLLGNVVMALIIGREHEDLNIRSAWLHVLGDTLSSLGVIISG
                     LIIYFTHWVYADPVASALIGIVILSGGIRVVREASSIFMEMTPAAFHPEEVAKKICAM
                     DEVMGVHDVHIWAVAHKHIAFSAHIWVHDQKLSEAEGIRKRIEGMLTEMGISHVMLQF
                     ECAECETNALYCQTHTTDTYHAH"
     gene            17589..>20196
                     /gene="secA"
                     /locus_tag="VMU21_11160"
     CDS             17589..>20196
                     /gene="secA"
                     /locus_tag="VMU21_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HUO78121.1"
                     /translation="MVQTLLKKIFGTKNEREMKRLWPMVDKINALEPQFASLNDAQLK
                     EKTPEFKKRLEEAESVDDILPEAFATVREVSKRTLRMRHFDVQLIGGIVLHEGKIAEM
                     KTGEGKTLVATLPVYLNALTGKGVHVVTVNDYLARRDARWMAPIYHFLGMSVGIIVHG
                     LTDEERQAAYNADITYGTNNEFGFDYLRDNMKYDISHYVQREPHYAIVDEVDSILIDE
                     ARTPLIISGPSEESTGKYYTIDKIIPKLQKDTDFTIDEKARTAILTEEGILKVEPLLG
                     VPNLYDPANIELVHHVLQALKAHTVFKRDVDYVVKDDEVLIVDEFTGRLMPGRRWSDG
                     LHQAVEAKEGVKIESENQTLATITFQNYFRMYQKLAGMTGTADTEAEEFAKIYNLDVL
                     VMPTNKPMIRADHPDMIYKTEKSKFNAVIKEIEELHQKGQPVLVGTISIEKSELLSHL
                     LKKKSIPHSVLNAKYHEREAEIIAQAGRSGAVTIATNMAGRGTDIVLGGNPEGLARDL
                     LKEKKDFTDEEWKQSLQKAEELCIRDREKVLSCGGVHILGTERHEARRIDNQLRGRSG
                     RQGDPGSSRFYLSLEDDLMRIFGSDRISGLMSRLGMDEDVPIENKMVSKAIENAQKKV
                     EAHNFDIRKHLLEYDDIMNKQRSEIYSFRREILQGEGLKEKIFQLIDDTVDEVLQIYC
                     PEERHTEEWDMKGLKDSLYGIFSLSLNASPAGIESLRESLSSQLKEAYERKEADVGSD
                     TMRYLERVLFLQVLDTQWKDHLLAMDHLKEGIGLRGYGQRDPLTEYKKEAFDMFADLT
                     GRISVDTLSRIFKIQIKEEESVKKQHIQPKQVQYNRGVSSESLQPVARGRKVGRNDPC
                     PCGSGKKYKKCCG"
BASE COUNT         4902 a         5252 c         5209 g         4833 t
ORIGIN      
        1 cagcaacgca aagattactc ttatgagttt atgggcggta gcaaacaccg ctcttttaaa
       61 caacaggcct tctgccttcc ttttcagaaa atagtttcta aaggtgctat tgtgctggct
      121 cacgttaacc gccatcagct caatgactct tcggagatga cggttcccct gttctgaact
      181 cctgcttgtt ccctcggaaa cttcgggcag ctttcataaa agactctgaa gcagaatgtt
      241 tctatctccc cgatgctggg aacatgaata gcatccggtg cgacacagat accaaagtat
      301 ccttccttaa aatatggaca gctcacaaac acctccttga cttgaaaagt tgaactccgg
      361 tattttcaag ctcctttgta ttagattcct gattcatgca atagttcctt ctcgattgtc
      421 aaaaaaagga gtgccagggc cacggagggg gatagggggg aatcttttga cccaggcact
      481 ccgagggagg gctttttaaa tccgtttctt cctaatcatg cgtcttgcat gtcatgccgt
      541 tatagcgatt attggacagt tgcctgagta gccaccctat ctgttaaaag ccacgtgatc
      601 caccaccgcg ggacttacgt ccactgtgtg tttcgttgtt ctctgccttt tctgtctgtt
      661 cttccctgag tttttgatac tccttcatct gctcctcagt gagaatcttt ccgatttgca
      721 tatcggtaga ccacttgagc tcctgcagtt gacttttcac tgccttcctg tcatctgaac
      781 tattcttcac tatctcacgg cgcttctcta tgcttccctc gataataggt ctcatctttg
      841 cttcctgctc ttcagtaagg ctgaggcgcg tctttaaccg ggacatgacc tcatcgggag
      901 aagcatttat gttcgatata tctcgcgctt gcgctccgat aggctgcgtc ataaagatca
      961 tggcacaaaa caatacgtaa ccagacaagg caaggaattt aaggtaactc ctattggcta
     1021 tgaccgccat gacgcatctt attgtggcca tcacgatatc gctcattggg tcactactca
     1081 taaaacctcc tctatctgtt cggtaaaaag atagacgatc aagaaaggtg ctcggttaca
     1141 atttcttcaa ggcatgaagt tccggatatt gtgagcgtga aatggctcgt atggagatgg
     1201 ggggggtgct gcctgccatt tcttggaggg gacggcaggc agcacgaggt aattcgaatg
     1261 gattttacat acagaatatg aagagaatat gaatgatcac aagaacagtt atttgtaaga
     1321 ataaaattca gctatggatg aactactcgg ctatgcgacc tacactttcc cgtatttgtt
     1381 gcagtgcaat gtcacttcag ttttgtaacc ttttgtacgt cttcaccgtc ttttaggcag
     1441 gtaaacatgt tttgaaaata tccgatcagg ctcatttcat atcggcattc tggagagttg
     1501 actcagcggg aattatttgt aaacttgagt tatgatatga aggcatcact tccgtactcc
     1561 aacactgtat gaatgaaggc tttaaggatg tcggtgagaa tgaagaaccc tgcggcaaga
     1621 ccgcagggta tcttaacgca aggagcaatt ttcattatat tcgctcaccg gcccgcagca
     1681 agctgcgggg aatgcactcg ctgcccgttt cacgacttgt agtgttcgtg gtgaaaggat
     1741 gaccaaaatg gctaccggag aactcaggga agacgaaagg gaaccgaagc cccatcccgc
     1801 ggcctcgcgt aagcactggt ggatctggct gacagcagtt tgccttatcg ccgtcgggct
     1861 ttacttttat ttcgctcaag cgccggctgg ctccgctaaa caaggtccaa acgtccgcac
     1921 ggtgcctgtg gtgacggcag cagcgagaaa gggcgacatc aggatatatc tcaacgggct
     1981 cggctcggta acgccgctca atgttgttac agtgaagagc cgcgttgacg ggcagctgat
     2041 gaaggtcctt ttccgtgagg gacaggtcgt cagcgcagga gacctgctgg ctgaaataga
     2101 cccccggccc tttgaggtgc agttgaccca ggccgagggg cagatggccc gcgaccagtc
     2161 actcctgaag aacgcagtgc tcgacctgga gcgctataaa gagttgtcgc agcgggattt
     2221 gatccctaag cagcagttgg atacacagga ggcactggta cgtcagtatg aaggagttct
     2281 caaactggac caggggcaaa tcgataacgc caagctgcag cttatttact gccatatcac
     2341 cgcgccgatt agtggacggg tcgggcttcg cctagtggac gccggcaata tcattcacgc
     2401 aagtgatcca aacggtttgg ttgtgatcgc gcaacttcaa cccatcacga taattttttc
     2461 gattcccgag gacaacctga cagagttatt acgcaagctt aaggctgcca agggcatacc
     2521 agtggaggcc tttgatcgcg agcagaatca gaaaatcgcc tccggatacc tgcttacagt
     2581 cgacaatcag atcgatccga caaccggtac ggtccgcctc aaggcaatgt ttccaaataa
     2641 ggagaatgaa cttttcccta accaattcgt caacgcccgc cttcttctgg acacgaagca
     2701 tggtaccact attgtgccgc aagccgcaat acagcgaagc ccaaaagggg cgtttgtgta
     2761 tgtggtaaag gcagacaaga ctgctgaagt gcgtccggtc agagcgggtc cgtctgaggg
     2821 agacgatatc tccatagacg agggcctgtc tcccggcgag atagtcgttg tggagggcgc
     2881 agacagactg cgagagggca gcaaggtcga actgcagggt caggcacaaa acaactcccg
     2941 caagggcggg tagatgaatc cttcgcgtag cttcatagtg cggccagtgg caacaacgct
     3001 gctgatggca gccattcttc tggccggcgc agtggcatac cgactgttgc ctgtctctgc
     3061 cttaccgcag gtggattacc cgacgattca ggtcgtgact ttttatcctg gcgcaagccc
     3121 ggatgtcacc gcttcatcca ttactgcgcc tttggagcgc cagttcgggc agatgccggg
     3181 actgcgacag atgacgtcca caagttccgg cggaagttcg gtaatcacgc tgcaattcag
     3241 tcttgacctg agcctcgatg tcgcagagca ggaagtgcag gcggccatca atgccgcaac
     3301 cacatttctc cccagagacc ttccgaaccc tccagtctat agcaaggtga accctgcaga
     3361 tgcaccgatt ctgactttgg cgttgacttc aaataccttg cccttgcccc aggtggaaga
     3421 tctggcagac acaaggcttg cgcagaagat atcccagtta tcaggcgtgg gactggtcag
     3481 tatcagcgga gggcagaggc cggcggtcag ggtacaggcg aaccccacgg tcctggctgc
     3541 ctatggactt acccttgaag acatgcgcac ggcccttgcg gcggcaaatg tcaatcaggc
     3601 gaagggtggc ttcgacggtc cacgccagtc ctccatcatc ggggcaaacg atcagctcct
     3661 tacgagcaaa gaatacaggc ctctggtcgt tgcctatcgt aacaacgcac cggtattgct
     3721 gtctgatgtg gctactgtca tcgatgatgc agaaaatgtg aatcaggcgg cctggatgaa
     3781 cgatgcccct gcggtgatcg tgaacataca gcggcagccc ggcaccaatg tcatagaagt
     3841 cgtagaccgc gtgaagaggc tgctgcctca attacaaagc tcattgcctt ccacagtgca
     3901 agtatccatt ctcactgacc ggacaacaac catccgcgca tcggtcaaag acgtgcagtt
     3961 tgagctgatg ctggcggtgg tcctggtcgt gatggtaatc tttttgtttc ttcggacatt
     4021 ggcggcaaca tctgtaccca gtgtcgcagt tccattgtcc ctcgtcggca ctttcggaat
     4081 aatgtatctt gtggggttca gcctgaataa cctctcgctg atggcgctta cgatcgcgac
     4141 cggctttgtg gtcgatgacg cgattgtgat gatcgaaaat atcgcgcgct acatagaaca
     4201 gggggagccc cctcttcagg ccgcgctcaa aggttcggaa cagatagcct ttaccattat
     4261 gtccctgacc gtctcgctcg tagcagtgct cattccgctt ctctttatgg gggatgtggt
     4321 gggacggctg ttccgcgagt tcgccatcac cctcggggtc acgattgtca tctcagcggt
     4381 agtgtcgctc actcttacgc cgatgatgtg cgcgagactg ctgaagcata agcctgaaga
     4441 gcagcaaaaa aggcttttcc gggtttcaca gcagctgttt gacaggacca ttgagttata
     4501 cggaaaaacg ctgaaatggg tgcttcagca ccagactccc acgctgttgg tggccgtggg
     4561 aactctgatt ctcacggtat ttctctacat ggtagtgccc aaaggcttct tccctgttca
     4621 ggacacaggt ttgatcctgg gtatatccga agcgccgcaa tggacgtcgt tcaccgcaat
     4681 ggcggagcag cagcaggcgc tcagcagagt gatcctaaaa gacccggcgg tcaaaagtct
     4741 ctccacattc atcggagtgg acgggacgaa cacaaccctc aacagcggac gggtactcat
     4801 taacctaatg ccgatcgaag aacgaaaaat gagtgcgagc gatatcatac gccggatgca
     4861 gccggaactt gcgaaactca gagggatcac gctttacatg cagccggtgc aggaccttac
     4921 ggtcgagagc cgggtgagca ggacacagta ccagtacagc ctcgaagatc ccgacatcaa
     4981 cgaactgaat agctggaccc ccagggtcgt cgatgccctg agcaggcttc cggagcttcg
     5041 cgatgtgagc agtgaccagc aggaccaggg tctgcgtgct tcattgctta tcgaccggac
     5101 cacagcatca cgattaggga tcacccccca gctcattgac gatacccttt acgatgcctt
     5161 cggacagcgt caggtttcta ctatgttcac gcagctgaac cagtaccgcc tcattttgga
     5221 ggtaagacct gaattcaagc aaaaccccgg caaactgaaa gatatctaca tccgcagctc
     5281 tggcggcgga caggtgccgc tgggcgcgat cacgcgcacc gcagaaacca ccggaccgct
     5341 cgtcataagc catcaggggc agttccctgc aacgacaata tcgttcaatc tggcgccggg
     5401 ggagtcgctc ggcaaagcga taaaagagat cgattcgatc acgcagcagc tggggctgcc
     5461 cgcaagcatc cgcaggagtt ttcagggcac agcccaggcg tttcagtcct cgctggccaa
     5521 cgagcctctg ctgatactag ctgcgctgat aaccgtctat attgtgctcg gggtgctgta
     5581 cgaaagttac atccatccga tcactatttt atcgacgttg ccatccgccg gcgttggtgc
     5641 gattctcgcg ctgctcatct gccgcactga attcagcgtt attgcattga tcggcatcat
     5701 tctcctgatc ggtattgtga agaagaacgg cattatgatg gtcgactttg ccctcgatgc
     5761 agaacgcagg gaaggcaaaa gcccggttga tgcaatctat caggcgtgtc ttttgagatt
     5821 ccggccgatc atgatgacca cgctgtccgc cctgctggca gcgcttcctc ttgcattggg
     5881 ctcggggatg ggtgcagagc tgcgccggcc gcttggtatc tctatcatcg gcggtctgat
     5941 cttcagccag atgcttacac tctacacaac gcccgtcatt tatctttggt tcgaccggtt
     6001 ggcgagacga ctaggcaggg tacgctccga aaattccgat gatgttccag atgaccgggc
     6061 cggagggaca ggctcttaat gaatatctcg gcgcctttca ttaagaggcc tgttgcgacg
     6121 acgctcctga ccgtcgggat ggtcttagtc ggaattgttg ccgtccgttt gctgcccgta
     6181 gctccgttgc ctcaggtcga ctttccgacg atctctgtat cagcggcgct gccgggggcg
     6241 agccccgaga ccatggcgac atccgttgct gctccgcttg aacgccagtt tgcccacatc
     6301 gcgggcgtga ccgagatgac ttcaaccagc acccttggag caacttccat caccctgcaa
     6361 ttcgaattga gccgcaacat cgacggcgct gcccgtgatg tccaggctgc gatcaatgca
     6421 gcgcgaagtt acctgccggc ggcccttccg agcaatccga cttaccgaaa ggtcaaccct
     6481 gccgatgccc cgattctcat tatagcgctc acctcggaca cggtgggtag agggcgaatg
     6541 tatgatgtgg cttcgactat tctccagcag aaactttcac aggccgaagg cgtcggccag
     6601 gtgatcgtgg gcggcggctc gctgcctgca gttcgcgtgg agctgaatcc tacggcgcta
     6661 aacaaatacg gcttcggtct tgatgacgtg agaaccgcat taaacagcac taatgcgaac
     6721 cgacccaagg gacaattagc ggacgggacc aggacatggg agatcactac taacgatcaa
     6781 atgtacaagg ccgaagagta taagcccctt attgtcgcat accggtctgg cgctgcggtc
     6841 agactttctg acgttgctga cgtgcaggat tcagtggagg acattcgcaa tgcaggactc
     6901 gttaacggac aaccggccgt gcctgtgatt ctcttccggc agcccggagc aaatatcata
     6961 gagactgtcg accgtatcaa ggacatgctc cctcagttgg aggcatctat ccctggcgct
     7021 gtcaggcttt cggtagtcat ggaccgaact acaactatcc gtgcctcatt gaaacacgta
     7081 gagcatacgc tcgttatcgc gggtgtactc gtgatcatgg ttgtcttcgc ctttctccga
     7141 aatatccggg ccacgcttat cccggctgtt gcggttcccg tatcgcttat cagcaccttc
     7201 ggggtcatgt acctctttgg atacagtatc gataacctgt cccttatggc gctgactatc
     7261 gccacgggct ttgtcgtgga cgacgccatt gttgtccttg aaaatatcac tcgtcacctc
     7321 gaaaaggggg tccccgcccg gcaggccgcc tacctgggcg cgcgggagat cgggtttacc
     7381 gtgctctcaa tcagcatttc gctcgtggcg gtgttcattc ccatattatt aatgggcggg
     7441 atgctgggcc gtctgttccg cgagtttgcg gtaacgcttt cagcggcaat cctggtttcg
     7501 ctcgtggtat cgctcaccgc aacgcctatg atgtgtgcgg cgctcctgaa accggagaca
     7561 gcccgttccc acggtaggct gtataaggcg ggcgaacggg tgttcgactg gatgcacagc
     7621 cattacgaag aaactctgtc atgggccctc cgccatcccc gattcatgct cgccctgacg
     7681 ttgataacca tagtgaccaa tatttttctg tttgttgtcg tgccgaaggg gtttttccct
     7741 caacaggaca ccggacgtct gaccgggtct attcaggctg cacaggatat atcgtttcag
     7801 gccatgaagg agaaactgac ggcaattgtg aacattatca aggacgatcc ggccgtagac
     7861 aacgtgatag cgtttactgg cggcggaggc ggaggtgtaa caaggaacac ggcccgaatg
     7921 ttcatagcgc ttaagccgca gggagaacgg aagctcagtg cggaccaggt gattgcgcga
     7981 ctgcgcaaga aactcgttag cgtaccgggt gcgccggcct tccttcaggc tgtacaggat
     8041 gtgcgcgtgg gaggaaggat aagcgccgca caatatcagt atacgcttca gggagatgac
     8101 ttgaacgagc taatggcgtg ggccccgcgg atggagcagc aattgcgcgc gttgcccggg
     8161 gtagtcgatg taaacagcga tcaacaggat aaagggatgc aatcgacggt agtggtcgac
     8221 aggagtaccg catcacggct cggcataacc tcacaggtga tcgatgatac cctctacagc
     8281 gcttttggtc agcggcaggt ctcgatcatg tatacattgc tgaaccagta tcatgtggtt
     8341 atggaggtag agccccggtt ctggcagcgt cccgaatctc ttcggaacat ttacgtccat
     8401 tcttcgaacg gagcgctggt tccgctgagc acgtttagcc gctatgagcc tactgcaacc
     8461 gcgctcgcgg taggccatca gggacagttt ccatcggtga ccttgtcgtt caatctggca
     8521 ccgggagcag cgcttggtga tgcggtgaag aagatcgaga cgaccactcg gaagatgggt
     8581 ctgccggcaa gtatcagggg cagtttccag ggtacggccc aggtctttca atcttcttta
     8641 gcaagcgaac cactgttgat tttggccgct ctggtctcgg tctatatcgt tctcggcata
     8701 ttatatgaaa gctacattca tcctattact atattgtcaa cgctgccatc ggccggtgtc
     8761 ggcgcgattt tcgcgctgtt gatctcccgc acggaattga gcgtcatagc aatcatcgga
     8821 attattctcc tcatcggcat cgtgaaaaaa aatggcattc tgatggttga cttcgcgctt
     8881 gatgcagaac gcagggaagg caagagttct gttgacgcga tctatcaggc ctgcctgttg
     8941 agatttcgtc cgatcatgat gacgactatg tctgctctgt ttggggccgt gcctcttgcg
     9001 gtaggatttg gcgtcggggc agaactccgg cgtccactcg gtattgctat tgtcgggggg
     9061 ctgatcttca gccagatgct cacgctctat acgactccgg tggtatacct ttaccttgac
     9121 aggtttaggc tttggctgga aaacaagagg aaaaaaatgg gatgggtcag acgaggtatt
     9181 gtttcgggac ctggggaaat gtgatggtgg agagatccgc gattgccggc gaaaaacgga
     9241 aagaatgatc tatatttttg gccgccgcta atctttatcc gcaagccgcg ggaaaaccat
     9301 attctcatta tattttagag gtttaatccc agtcgaggag cctctgcttg ccttccagtt
     9361 tgcgtatttt cgtcacgtcc tggctcactt ccagggtgcc cctgtatttt ccccctgaat
     9421 cgcgcaccgc gaaatacctg atgtggataa acctgccctt catctgaatc cagaactcag
     9481 caacatccct tttccctgcc ctgaattcat ccaatatctt ctgcaccaca tggaagctct
     9541 tgggagggtg gcacttctgc acttttcttc cgatgacccc cgggcttctt gggaaaatcc
     9601 tctcttttgt cgcggaataa tacacaagct catccttctc atccacatac gagatgtcaa
     9661 ccggcaggtg ggtaagcagg agatccacct gttcccgggt cagacggcct gtgctgagat
     9721 ccacagcgcc tgactcaaca gctgctcttg cctcagcagg aacctcagct cctggcatcc
     9781 aacccttttc aggctcaacc catgcatatc ctatctcctc ttcaccctgc cgcaccttta
     9841 tccaatcagc ctcggccagc atctccattg ccatgggaaa gaggatatgc tcttccttat
     9901 aaatcatgtc gttgatgctc tggagcagat atttcagcgt gccatacaca agagggacct
     9961 gaagctcaga tatctgcgcc ctcgcgtatt tcaatgtctg acgtatgtcg tcgtggattg
    10021 cccacatcac ctcagaaggg ccgctgatgc catgtttttc cagcacagga aaaagctggt
    10081 tctccttccg gagatagtga aggttgatgt ccgaaagtct ctctatcagc gctcccagat
    10141 ttttcctgtg ctgacggaag acctctttgt ccgacgtatc gcaaagattg tccatgatac
    10201 ctttaatctc atggacaatc ccttccacgg ccctgttttc cttcataaag gtatggaggg
    10261 gatgtccggg gggtgcagaa ggcgcctcct gggctcccaa cgcctctttg aaaacctcca
    10321 catggacatc gcagagacgc tttatctcgg tctcaggcat tccctcttcc atgagcttct
    10381 gctccatcct tgctatctcg gtggcatcaa tgtcttttat aagctcataa aacctctgct
    10441 tcaggaccgc catgtccaca cccttatgga ggtccctgat aatgtctttt aggacttcct
    10501 ggcgcatctc agcgtcctgt atcggcccag cctccgtgct ctcttccaca atcgggacgt
    10561 cctgtcccgt cttttccttt attgtggcgg ctatttcttt cagcaagagg gggagttcaa
    10621 tgcccccgat gtgggcaacg tgtgacaggg aagcgacttt ccccactgtc tttctcatga
    10681 ccgtactctg gagcatcttg aactttggcg aacgattgat gaaaaactcc atcagaaaag
    10741 gatagtcttt cagcaactcg tcaatcttgg tctttccact cagttccatt cttccccctt
    10801 atctgttctt ccgtatttct tgcctacatt cacactctaa aattatatca gattaatttt
    10861 ttcaatccat tgtgaccgga ttctcgtacc tgcttctgaa aatccggaca atcagtgcgg
    10921 cgcaccaagg agtattgacc cctgagtaaa aaaagtccct gccactgcag cagtcatgag
    10981 acaggcaaga gtcgcggcaa taagcgccct gaagccgatg gctgagatgt cttttgtccg
    11041 tttcgggact agggccgcgg tgcctcccac gaataccgca agagaggcga agtgggcaaa
    11101 cccgttaagg gcataggcag caagcaccgc cgaacgggga ttcttcaggc ctcctgtaga
    11161 gaggagcact gcgaggtcct ggtacgactt cacctcagtg agaaccgctc tttcgccgat
    11221 aagctttgag atttcaaagg catctgaagg gggaacccct atcgcaaggg tgagagggta
    11281 aaagagatag cccaggagac cctttaatga ccagtccatc tggaggccgg cgaagttatt
    11341 cagcctccct cccagacctc caaggaggga atccacaagg gccacgatac cgaggaatgc
    11401 caggagaagg gccatgatcc ctaccaccat cttcatcccc tcattcgcgc cgttgatgat
    11461 cgcttccatc acagtggact ctttttggta atgaggagct atctccagcc caagggtctc
    11521 cggtgtttcg gtctcaggca tgatgatctt cgatatcaca atcgcgccaa tggcagagag
    11581 gatggaggaa gagatgagat gacccgcaat ggtggggaac cgtccctgga ggagcatgac
    11641 gtaaagggcg aggacattcg atgcagtggt agccatgcct gctgaaagta tggtacagag
    11701 ctcagagcgg gtcatgcctc cgagaaaggg aaggatcgtc atattggcct caacacccac
    11761 gaagatgttg ctcgatacgc tgagggattc cgcaccgctt atcctcatca gtttcgtaaa
    11821 cgtcgtcgag aagagcttaa tgagcctggg cattaccccg atatagtaga ggagcatcat
    11881 gagactcgaa aaaaagacaa tagtgggcag ggcctgaaag gcgaggaaaa accccaggga
    11941 ctcttctcct gcctcattct tcgtccccgg cggaagcgca agcctgccga agagaaacct
    12001 ggtccctgca ccagcgctgt cgagcacctt caccaccgca tcattgatca caagaaatag
    12061 tcgcgagcct acgggcacca caaatatgaa aaacgcgaag acaagctgga gtgtcagacc
    12121 ccagacaata acccgccagt tgatcacttt tctatgcgct gacattgtcc acgcaacgaa
    12181 catgaggaca aatatccctg taaaactcac cagattatcc atgaatgtcc ctcaccttat
    12241 ggtactcctt cgccctactt ccttttctca aacaacaacc ctgagatggt cacgaacacc
    12301 gctgaggtgg cgaccaaaac cccgatgtcc atgaaaaggg aaaaatcaaa taccccttgc
    12361 tccctgaaga ttgcgtgctt cagtgcgtcg attccgtacg tgaaagggtt tattcttgtg
    12421 agcagtttca ggattgaggg aagcatcttc accggataca tcgcaccgga aaggaaaaac
    12481 attggcatca caataaagtt catgatcacg ctgaagctct caaaactctc ataaaatgtt
    12541 gcgatcacga ttccaagtga cgcgatacag aaggacagca gcgtacagat caccaatgcc
    12601 tcaatgatcg gtaggacccc gagtctgata ccaaggaaag gaaagagggc aaggattatg
    12661 gctgcctgaa tcaccgaaat tatggtcccg ctcagtgcct ttcccagcac tatggagagc
    12721 ctggaaaccg gtgccacgag tatctctttc atcagcccga actctttgtc ccatattatg
    12781 gatatggaag agaatatcgc actgaagaga atcgtcatcc cgagaatccc tggaaagata
    12841 aattggatgt aggagatacc catgccgggg gagacgattc ccatgcccgg ggagacgagc
    12901 ctcgacatgc cgccacccac gaggaagagc cagatgagcg gtctcgcgag tgtcgagacg
    12961 agcctgctct tttcccttat gaatttcttg aactcccttg ccacaaggac atagatggca
    13021 ttaccctctg ccaaatctcc tcctgtatga gcggacttcc tctttgatgg tgtctccgga
    13081 cgatacccct tcagccctga tttcctttcc ggtcatcttg aggaaaacat cattcagcgt
    13141 tggtctctgg agtctcaccg aaaccactgt ttcgcccata gccctgatga tttcggggat
    13201 acacgcgtct cccttcaagg tggtaaggaa gagttcgccg tcctgctcgg acacctccac
    13261 atggaacagc ttctcgatct cccgccgtgc cgtgagattg tcagttgtcc tgatatagat
    13321 cacgtctccc cccaccgttt tcttcagctt ctccggggtg tcgagggcaa ttatcttccc
    13381 tttgtcgata attgcgatct ggtcacatac ctcagcctcc tccatataat gggtcgtcat
    13441 gaaaatcgtc acttgatgct ctttgggcaa tgcgacgata aactcccaga gatttgccct
    13501 tgtctgggga tcaagcccca gtgtgggctc atcaaggaac agtaccttcg gcctgtggat
    13561 aagtccacgt gccacctcaa gtctccgttt cataccaccg gagaatttct tgacaaggtc
    13621 attcctcctg tcgtagaggt caacaaaacg aagggcatct tcgatccgct gcttcctttc
    13681 atccttatcc acattgtaga gatacgcatg aaataccaga ttttcgtatg cggtcaggtc
    13741 cttgtccagt gttgaatcct ggaagacgat gcctatggat ttccttactt ccggggattt
    13801 cgttgtgcag tcgtgtccat aaatggatgc ttttcctgag gtcggggaga gtaatgtgca
    13861 gagaatattt attgtggtgg tcttccctgc tccattgggg ccgaggaagc caaatatcgt
    13921 gccctcctcc acctcaaagg aaatatcatt taccgcagtg agattcccga accgcttgac
    13981 aagatgctca accctgatga taggcatata agagtttacc agaaaaggac ggggatggaa
    14041 aatgacgact actacattat agaaggacgc atggcatggc cattgacctg ggtggtgatt
    14101 cttggcaccc tgccgggagt attcatcgcc tatttcataa gggtcctcta tcttcctgat
    14161 ccggggacat tcagattatt cgtgggctgt gtgcccctct ttattggggg cgcagactgt
    14221 tttatgaatt aaagaggagt gctgagtcag gcaattaagg gaaaagtgct cctgaaaaaa
    14281 ttcaaggaca gggtgcagag atcggaagac agagcgtatt gctcaaactc gtttgctcct
    14341 caacttgatt caccttctcc cccatcgata ctccttctca ccatgtttgc catttcagcc
    14401 atcctgtaag gcttgtcgag aaatcccatg acgcccaact ccttgagatt cagtgcctca
    14461 tcagccgcat agccactgga aatgatgact ctgacacggg gatttacctc tttcaggagt
    14521 ttatatgatt cgatgccgtc aatcttgggc atgaccctgt ccataatgac aagggcaatc
    14581 tcatcatgcc gtttaatgta aatccgtaag gcttcctcac catttgcggc ggtaatgacc
    14641 tcatacccaa gcccatggag aatggatttg cccaggtccc ttatcacctc ctcatcatcc
    14701 actaagagaa tgcattcttt tcccctcgga agcgcctgtt tctgtgatat gtcccttgac
    14761 ttcactacgg tatcaggccc cggaccatag gcagggagat atattgtgaa cgctgtcccc
    14821 tttccttctt cggtgtaaaa gtttatccag ccaccatggg cccttatgat ggaatagctg
    14881 actgcaaggc caagaccagt gcctttgccc tttaccttcg tggtgaaaaa cggctcaaat
    14941 atcctcgtct ttatctcctc ggaaatacct tctcctgtgt cagatactgt tatggtcaca
    15001 aaataccctt gcgccgcata tgcataatac gtgcagtact cctcatccac atgggattcc
    15061 ccggttctta tctcgatcgc tccgcccttt ggcatagcat ctcgggcatt aaccacgaga
    15121 ttcatcagga cctgtgtgat ctgggattga tccgcattca ccatatgcag gccggtcctt
    15181 ggtattagac gtatgtcgat gttctcctcc accattcgcc tgagcatcct catggactcc
    15241 tcaataagcc ggttaatatc ggtgggttgc cgcgccgggg gaagcttccg cccaatgagc
    15301 aggatctgtt tagtgaattc agtggcattc tgggtaagct caaggaccct ctgtaggtgt
    15361 tccttcaccg gctggtgtgt cgtctcatcc agggcaagct cagagtaccc gagtattccg
    15421 gtaaggatgt tgttgaagtc atgggcgatc ccggctgata aggtaccgat cgcctccatc
    15481 ttctgggcat gaaggagaga tgtcctgtat tcatccaatt ccttcgtaac cgcattactc
    15541 ttctcctgca ggttctttgt catggaatta aagcttctcg cgagcattcc cagttcatct
    15601 tttgcgtcta tttcaatggg atgggagagg tctcctgcgg tgaccctttg aattccctct
    15661 ttgagaagca ccacagggct cagaacgaag tgttgcacaa caagatagag agggacaaaa
    15721 agaacggcga tgaagacaaa accaaatgcc gctaatgtat acctctgctt actgatataa
    15781 gcgtcaacag agcgcaagga gtactccgtc tcgatcctcc ccaatatttt ttggtctttt
    15841 gaatgggcat ggcacttcgc ggtatagcag gggtgcatat tatgtatcgg ttggatgatc
    15901 tcaagcaccc tctgacccct caccttcttc atcatcgacg ttcgaccttc ctcgtctcga
    15961 acgttgctta tggcactgtc cggccttgca cccgtctcac ccctcactga agaaaaatga
    16021 acaccgcctt caggatcagt aatcctgatc catagaatat tgtccatgga agccaacgat
    16081 tcgattgtct gcttaataaa ctctctgtta ttggtgagca ttccatagtg gagggccctc
    16141 tgtataacgt ccgtctgtga ttcggcgaaa tttatgtttt tgttaagttc ttccttttgg
    16201 atatggacaa tggaggggag ccagaagaga atagttatcc cgacgatgat gagtacgctc
    16261 gtaatgagga gtttgcttgc gagagaaagg cgtagttttt ccgtcatcgt accgctctca
    16321 cctcctaaaa ggctagtccg ggacttctgt ataataatac aaaaggatga aaatgcatac
    16381 attcttataa gcaattctca tgccttcaat acatcgaatc catcccagaa aatgcaaaac
    16441 cataattatg cctaaatata tgatataatt aacaaaaaat tatgtctttt caaagcgacc
    16501 attctcaagg ataaggaatg ttgccagaca tttgtcaaac aagtgaccag ggtgtcttgt
    16561 ttccgggcga tttgcctccc cagttgcagg gactaagggt gccatcatga gcctgagcgg
    16621 ggagaagcgt cttggcctga gtcttgcggt aacggctgcg atattgattg ccgaagtggc
    16681 agggggcctc ctgagcaaca gccttgcgct tttgagcgac gccgggcatg tgctcaccga
    16741 cagctttgct ctcgggttaa gtatggtggc tgcgcggatc agcaggaaac cctctgatta
    16801 cagggcaaca tatggctatc agcgcgtagg actcctggct gcagtgatta acggattgag
    16861 cctcgtggcc atttcagcat ttatcttctt tgaggcgtac cagaggatta cctctcctcc
    16921 ctccataaac gttcccctga tgcttctcat cgcaacatta ggactgctcg gcaatgtggt
    16981 gatggcactc attataggaa gggaacatga ggacctgaac atcagaagcg cgtggttgca
    17041 tgtgttgggg gataccctct cctccctggg ggtaatcatc tcagggctta ttatctattt
    17101 tacccattgg gtgtacgcag accctgtggc cagcgcactt atcggtattg tcatcctctc
    17161 aggagggata cgggtggtaa gagaggcctc ctccatattc atggagatga cccccgcagc
    17221 gttccatccg gaagaggtcg caaaaaagat ttgcgcgatg gatgaggtaa tgggggtgca
    17281 cgatgtccat atatgggcag tggcgcataa acacatcgcc ttttctgcac atatatgggt
    17341 tcatgatcaa aaattaagcg aagcggaagg cataagaaaa aggatcgagg ggatgctcac
    17401 cgagatgggt atcagccatg tcatgctcca gtttgagtgc gccgagtgcg aaacaaatgc
    17461 cctatattgc cagacccata ccacagatac ataccacgct cactgagaag acctcccccc
    17521 tcagaatcca gacataagtg ccccaagttt tcttgcaatt atcgggaatt attatttatt
    17581 attaatctat ggtacagaca ctcctcaaaa agatctttgg gaccaagaat gaaagggaga
    17641 tgaagcggct ttggccgatg gtggacaaga tcaacgctct cgaaccccaa tttgcgtccc
    17701 tgaacgatgc acagcttaaa gagaagacgc ccgagtttaa gaaaagactt gaggaggcgg
    17761 agtcggtgga tgatatcctt cccgaggcat tcgcgacagt gagggaggta tcgaagagaa
    17821 ccctcaggat gcgccacttc gatgtccagc tcattggcgg catcgtgctc cacgaaggaa
    17881 agatcgccga gatgaagaca ggagaaggaa agacccttgt ggcaaccctg cccgtctatc
    17941 tcaatgcgct taccggcaag ggcgtccacg tggtcacagt gaatgactac cttgcaagga
    18001 gagacgcgag atggatggct cccatttacc attttcttgg catgagcgtc gggataatcg
    18061 tacacggcct cacggatgag gaaaggcagg cagcctacaa tgcggacatc acctacggaa
    18121 caaacaatga attcgggttt gattatctgc gagacaacat gaaatatgac atatcccact
    18181 acgttcagcg ggagccgcac tacgccatcg tggacgaggt agacagcatc ctcatcgatg
    18241 aggcgagaac accgcttatc atttcagggc catccgagga atccaccggc aaatactaca
    18301 ctattgacaa gatcattcca aaactccaga aagatacgga tttcaccatt gatgagaagg
    18361 cacggacggc gattctcact gaggagggaa tcctgaaggt cgaaccgctg ctgggcgtcc
    18421 cgaacctgta tgacccggcc aatatcgagc ttgtccatca tgtcctccag gcactgaagg
    18481 cccataccgt tttcaagagg gatgtggatt atgtggtaaa ggacgacgag gtcctcatcg
    18541 tggatgagtt cacgggaagg ctcatgccgg ggaggcgatg gagcgatggc cttcaccagg
    18601 cagttgaggc aaaagaaggc gtcaagatcg agagcgagaa ccagaccctt gccaccatta
    18661 cctttcagaa ctacttcaga atgtaccaaa agcttgcggg catgaccgga actgccgata
    18721 cagaagctga ggagtttgca aagatctata atctcgatgt cctcgtaatg cccaccaaca
    18781 aacccatgat cagggcagac catccggata tgatctataa gacggaaaag tccaagttca
    18841 atgcggtcat aaaggaaatt gaagaactcc accagaaagg gcagcccgtg ctggtaggca
    18901 ccatatccat agaaaagtca gagctcctga gccacctgct gaagaaaaag tctatccctc
    18961 actcggtgct caatgcgaaa taccatgaga gagaggctga aatcattgcc caggcgggaa
    19021 ggagcggtgc ggtgaccatc gccacgaaca tggcgggaag aggtacagac attgtcctgg
    19081 gaggtaaccc cgaaggactc gcgagagacc ttttgaagga aaagaaagac ttcaccgacg
    19141 aggaatggaa gcagagcctg cagaaggcgg aagaattatg tatcagggac cgggagaaag
    19201 tgctttcctg cgggggagtc cacatactgg gtacagagag acacgaagca aggagaatcg
    19261 ataatcagtt gaggggccga tcaggcaggc agggggaccc gggatcttcc cggttttacc
    19321 tgtcccttga agatgatctc atgaggatat tcggatcaga cagaatctcg ggcctgatgt
    19381 ccagattggg tatggacgag gatgtgccca tagaaaacaa aatggtctcc aaggcgattg
    19441 agaatgcgca aaagaaggtg gaagcccata attttgatat aagaaaacac ctcctggaat
    19501 acgatgacat catgaacaag cagaggagtg agatctattc tttcaggcgg gaaatcctgc
    19561 agggggaggg cctgaaagaa aaaatattcc aactgatcga cgataccgtg gacgaagtgc
    19621 tccagatata ctgccccgag gagcggcata ctgaggagtg ggacatgaag gggctgaaag
    19681 attcccttta cggcatcttc tcgttatccc tgaatgccag ccctgcaggg atcgagagcc
    19741 tgagggaaag tctctcctct cagctcaagg aggcctatga aaggaaagag gccgatgtcg
    19801 gcagcgatac catgcgttat ctcgagaggg tgctttttct ccaggtgctg gatacgcaat
    19861 ggaaggatca ccttctcgcc atggaccatc tgaaggaagg aatcggactc aggggatacg
    19921 gacagaggga tcccctcaca gaatataaaa aagaggcctt tgacatgttc gccgacctga
    19981 ctggcaggat ctccgtagat accctcagca gaatctttaa gattcagata aaggaagaag
    20041 aaagtgtgaa gaaacagcat atccagccaa agcaggtgca gtataatagg ggcgtgagtt
    20101 cagaatcact gcagcctgtg gcaaggggaa ggaaggtagg aaggaatgat ccctgtccgt
    20161 gcggaagcgg caaaaaatac aaaaaatgct gcggga
//
LOCUS       DATKOU010000235         6477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_334096, whole genome shotgun sequence.
ACCESSION   DATKOU010000235 DATKOU010000000
VERSION     DATKOU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334096"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VMU21_11165"
     CDS             <1..357
                     /locus_tag="VMU21_11165"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HUO78122.1"
                     /translation="RRAGFATIFLLAPTSTGDRIRKVGKASRGFIYYVSMTGITGSRL
                     FLDDSFSESVQNIRRTTGKPVAVGFGVSTPEEAKVVSEISDGVIVGSAIVKKLHDSPD
                     TLESFILELRRAISQQ"
     gene            423..1271
                     /gene="accD"
                     /locus_tag="VMU21_11170"
     CDS             423..1271
                     /gene="accD"
                     /locus_tag="VMU21_11170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HUO78123.1"
                     /translation="MAWFKKTKEQQKIEKKVKIPEGLWVKCENCKEIVYKKEIDKNLR
                     VCPKCNYHFRISARERLKLMVDEGSFVETNSEIGPADPLAFKDSVLYKDRIRENQKRS
                     GLREAVITGEALINGNPMVVAIMDFSFLGGSMGSAVGEKIVRAAEAALEKKIPFITVA
                     SSGGARMQEGIFSLMQMAKVSAAVGRLKESGILYISILSDPTFGGVTASFATLGDIII
                     AEPRSLIGFAGPRVIEQTIKQQLPENFQRAEFLLEHGMIDMVVSRKDMKETLGKIIGH
                     LSPAPQ"
     gene            complement(1240..2499)
                     /gene="murA"
                     /locus_tag="VMU21_11175"
     CDS             complement(1240..2499)
                     /gene="murA"
                     /locus_tag="VMU21_11175"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HUO78124.1"
                     /translation="MDKLIIHGGRRLHGEVLISGAKNAALPIMVASLLAPGKSILRNV
                     PRLMDVLTMGKLLSSLGAGVHYESGTVSVDAGQVAGHEAPYNLVKTMRASVLVLGPLL
                     ARKGIARVSLPGGCAIGARPINLHLMGLEKMGASIELDRGYVIARSKGLKGSVIYLDT
                     PTVTGTENLMMAGSLARGTTMIENAAKEPEVIDLANALTSMGAIVRGAGESTIEVEGV
                     EELKPLSYSIIPDRVETGTFMIMAGITGGDILIKGCNPEHNEALITKLQEAGLVIEKG
                     SNQLQVTGPERPLARDIKTMPYPGFPTDMQAQFMALMSVAQGTSVIRETIFENRFMHV
                     AELKRMGAEIGTEGGVATVRGVKKLKGTEVMATDLRASASLIVAALAAEGKTTIHRVY
                     HLDRGYEKIEEKLKGLGATVERVKDAR"
     gene            complement(2514..3152)
                     /gene="gspM"
                     /locus_tag="VMU21_11180"
     CDS             complement(2514..3152)
                     /gene="gspM"
                     /locus_tag="VMU21_11180"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HUO78125.1"
                     /translation="MERSKTLMIAIPLMVILVGVVVYRYGYVRVRAELKTIKEDQAIS
                     MKTLEKYIALISEKPELEKRLSSLTEKRKADNAKLMEAQTPSLVAAALQDSVNGIVKG
                     RGGTITSERVGKPEDLGKFNVITVSMDVTLPDVSVLSDILSSIEAYTPSLVEKELDVR
                     IRDFRNPKELMVKVDVSAMTGGQPLPSPAQGAKSLPSPVQTQPATPKETAVE"
     gene            complement(3139..4695)
                     /locus_tag="VMU21_11185"
     CDS             complement(3139..4695)
                     /locus_tag="VMU21_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HUO78126.1"
                     /translation="MNGTKAIAIEKLTSAVSSSAARLKALWEPLWKTLSLSLADARIA
                     PAKNVTVCIERGLLSVVYGSRMLSRMKIRGFRLYPLDGRYPSAEGLASSVNVALSELG
                     ASAANITLSIPKAWTLVKRAEFPATVKENIANVISYELDRLTPFSAGDALYDAKLIRE
                     EAGKVTVLVSAAKADLVTPFLDALKEVGAHVTKLTMHLSGMGTLCRYTDKSEDFVFVK
                     MSGKEYEGAVFNGGSMTGTFTGTLEAEEEREKLDILMSEVEPLLERATTQGRPSKLFV
                     FLKDGSSSLREMLKMRVHAPLRILEETDVILGVPVQKIPYQAVGGLIESLWTKAAGPN
                     LLNKGREEKETTPLAGTFILAAILVLLWVIYIFSPVSVEERRLKEINRQIMIRKEEVK
                     KVEKVRKEVDALTSEVSTINDFKAARPTALNIIKELTTILPKSAWLTRIRITGTTVEL
                     EGYASSATELLPILEASKYFTKAEFASPTFRDARLNSERFSIKMEIEGVKKPQEVKKP
                     EGEKPKIGKK"
     gene            complement(4692..5639)
                     /locus_tag="VMU21_11190"
     CDS             complement(4692..5639)
                     /locus_tag="VMU21_11190"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HUO78127.1"
                     /translation="MKSQKGVALLMVLWVLTILMVIVLSFSFMARTETYATLAFKEKM
                     EMKFLAEAGIQRGIEEILYMKQNAQLPQFEGSELWKTDGTPYSGRLGEGEYEVRITDE
                     SGKIDLNTLNETSGIILKTLLTNMGVQDENADTIVDSILDWKDADDLVRLHGAESDYY
                     MSLPNPYKAKNANFDTLEELLLVKGMSYEIFFGTEGKKGLVDLITINSKMSVINVNAA
                     PKEVLMAVPGITPEIADAIISSRQDKKIMNLQEVGIPPQSAAYISIADGNAFAVEAIG
                     HKGEAKKGYPIKAVVTLVTLDSNKKYKYLYYKSPVTASR"
     gene            complement(5693..6355)
                     /locus_tag="VMU21_11195"
     CDS             complement(5693..6355)
                     /locus_tag="VMU21_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUO78128.1"
                     /translation="MRFFANSGIPHLSSRIGFTLLELMISIALIGLIVLIVAGAMRLG
                     YRSVDAGERKIESLERMRASLTLIDSQIQSAIPLTIDEDGSRKYYFKGEKGTLLFPSN
                     HSLWGGQMGYVVVTYSVVPDARGKQTLWISESIIGAENKIEAKLFESLDEISFQYFYK
                     GPTDEQGSWVEQWTDDTDIPEKVRVRLVQGPSELSLVIPMRARGYLAKLPSATVPQIQ
                     GK"
BASE COUNT         1387 a         1706 c         1678 g         1706 t
ORIGIN      
        1 cgacgtgcag gctttgccac catattcctc cttgcgccca cttcgaccgg agaccggatc
       61 aggaaagtgg gaaaggcgtc caggggtttc atctactatg tgtccatgac cgggatcacg
      121 ggctccaggc tcttccttga cgattccttt tctgaatcag tgcagaacat ccgcaggaca
      181 acgggtaagc ccgttgcagt gggctttggc gtctcgaccc cggaggaggc aaaggtcgta
      241 tcagaaattt ctgatggggt gatcgtggga agtgcaattg taaaaaaact tcatgattcc
      301 ccagacaccc ttgaatcgtt tatcctggaa ctgaggaggg caatctcgca acaatgatac
      361 gtgagtagtg atcagtaaga aaatagaact cttcactatt cacatccatg gttcatcaat
      421 ctatggcgtg gttcaagaag acaaaagagc agcagaagat tgaaaagaag gtcaagatcc
      481 ccgagggctt atgggttaaa tgcgaaaact gtaaggagat agtgtataag aaggagatag
      541 acaagaacct cagggtctgt cccaaatgca attaccactt caggatatcc gcacgagaac
      601 ggttaaagct tatggtggac gaggggagtt ttgtggagac aaattccgaa attggtcctg
      661 ccgatcccct ggcattcaag gattcggttc tctacaaaga caggatcagg gagaatcaga
      721 agaggagcgg tctgagagag gcggtgatca ccggtgaggc gctcatcaac ggaaatccga
      781 tggtggtggc catcatggac ttttcttttc ttggggggag tatgggttct gcggtgggtg
      841 agaagattgt gagggcagca gaggcggctt tggagaagaa aattcccttc attacggtgg
      901 cgtcctcagg gggggcaagg atgcaggaag ggatcttttc tctgatgcag atggcgaagg
      961 tgtccgcagc cgtgggtagg ctgaaggaga gcggaatact gtatatctca atcctctctg
     1021 atccgacctt tgggggtgtt accgcaagct ttgcgacgct gggagatatc atcatcgctg
     1081 aaccaaggag ccttataggc tttgcggggc cccgggtaat cgagcagacc ataaagcagc
     1141 agctcccgga aaattttcag agggctgaat ttcttcttga acacggaatg atcgatatgg
     1201 tggtgagcag gaaggacatg aaagagaccc tggggaagat tatcgggcat ctttcacccg
     1261 ctccacagta gcaccaaggc ctttgagttt ttcctcgatc ttctcatatc ccctgtccag
     1321 gtgatagacc ctgtggattg tggtctttcc ctctgcagcg agtgcggcga caataaggga
     1381 ggcgctggcc ctgaggtctg ttgccatcac ctcggttcct tttagttttt taacacccct
     1441 taccgtggcc actccccctt cggtcccgat ttcggcaccc atcctcttca gctcagccac
     1501 atgcatgaac cggttctcga atatcgtctc ccgtattacg cttgtcccct gtgcaactga
     1561 catcagagcc atgaactggg cctgcatatc ggtggggaat ccaggatagg gcattgtctt
     1621 tatgtccctt gcgaggggcc gctcaggccc ggtgacctga agctggttgg accctttctc
     1681 tataaccagt cccgcttcct ggagttttgt aatcagtgcc tcgttgtgtt cggggttgca
     1741 gcccttgata aggatgtccc cccctgtgat tcctgccatg atcatgaagg tacctgtttc
     1801 gaccctgtcg gggattatgg agtatgagag tggtttcagc tcttcgaccc cctccacctc
     1861 tattgtgctc tctcctgcac ccctcacgat ggcacccatg gaggtgaggg cattggcaag
     1921 gtcgatgacc tccggctcct ttgcagcgtt ctcaatcatg gtggttcccc tggcaaggga
     1981 acctgccatc atgagattct cggtcccggt cactgtgggc gtgtccaggt agatgacaga
     2041 gcccttcagt cccttcgagc gggcgatcac gtaccccctg tccagttcta ttgaggcacc
     2101 cattttctca aggcccatga gatggaggtt gatgggtctt gccccaatgg cgcatccgcc
     2161 ggggagagac accctcgcga tgcctttcct ggcaaggagc gggccgagca caagcacgga
     2221 cgcgcgcatg gttttcacaa gattgtaggg ggcttcatgg ccggcgacct gtcctgcatc
     2281 gacactcacc gttcctgatt cgtagtgaac cccggccccg agactggaaa ggagtttccc
     2341 catggtgagt acgtccatga gtctggggac attgcggagg atgctctttc ctggtgcgag
     2401 tagggaggcg accatgatgg gaagtgccgc attctttgcg ccgcttatca gtacttcccc
     2461 gtgaagcctc ctcccgccgt ggataatcag cttatccatt ttggtgtctt cgattattca
     2521 actgcagttt ctttcggtgt ggccggttga gtctggacgg gtgagggaag agatttcgcg
     2581 ccttgggcgg gtgagggaag aggttgcccg ccagtcatgg cagaaacatc cacttttacc
     2641 atcagttctt tcggattcct gaagtccctt atcctcacat caagctcttt ttccacaagt
     2701 gaaggagtgt atgcttctat ggagctaaga atatcactca ggacactcac atccggaagc
     2761 gtcacatcca tggaaacggt gatgacattg aatttaccga gatcctcggg tttgccgact
     2821 ctttcactgg tgatcgtacc gcctctgccc ttcacaatac cattgacgga gtcctggagg
     2881 gctgctgcca ctaaagaagg ggtctgggcc tccatgagtt ttgcattatc cgccttccgt
     2941 ttctctgtga gtgaggagag ccttttttct agttccggtt tctcggagat cagggcaata
     3001 tatttttcca gtgtcttcat gcttatggcc tgatcttcct tgatcgtctt cagctctgcg
     3061 cggactctca catacccgta ccggtagacc acaacgccga ccagtatcac catcaagggg
     3121 atggcgatca tcaatgtttt acttctttcc aattttaggt ttttctcctt cgggtttttt
     3181 cacttcctgg ggctttttca cgccctctat ctccattttt atgctgaacc tctcggaatt
     3241 aagccgcgcg tccctgaagg tgggtgacgc aaactctgcc ttggtgaaat actttgaggc
     3301 ttcaagtatg ggcaagagtt cggtggcaga actcgcatac ccttcaagtt cgacggtggt
     3361 tccggtaatc cttatcctcg tcaaccacgc gctcttcgga aggatagtgg tgagctcctt
     3421 gatgatgttt agggccgttg gcctggccgc cttgaaatca tttatggtgg aaacctcact
     3481 tgtgagtgca tccacctcct ttctcacttt ttcgaccttc ttgacctctt cctttcgtat
     3541 catgatctga cggttgatct ctttgagtct cctctcttcc acactcacag gggaaaagat
     3601 gtagatgacc cacaagagaa cgaggatggc ggcaaggatg aaagtcccgg caaggggcgt
     3661 cgtttccttc tcctcccggc ccttgttgag aaggttgggc ccggctgcct tcgtccacaa
     3721 agactcgatg aggcctccca ccgcctgata aggaatcttt tggacaggga cgcccagaat
     3781 gacatcggtc tcttccagta ttctcagggg ggcatgcact ctcatcttca gcatctctct
     3841 gagggaagaa cttccgtctt tcaggaatac gaagagcttt gaaggcctgc cctgcgttgt
     3901 ggccctctcc agaagcggtt ccacttccga cataagtatg tcgagcttct ccctttcttc
     3961 ctctgcctcc agagttcctg taaatgtgcc cgtcatggag cccccgttga ataccgcacc
     4021 ttcgtattcc ttcccactca tcttcacaaa gacaaagtcc tcacttttat ctgtgtaacg
     4081 gcaaagtgtg cccatgccgg agagatgcat ggtgagcttt gtgacatgag cgcccacttc
     4141 cttgagggcg tcgaggaagg gggtgaccaa atctgccttc gctgcactca ctaacacggt
     4201 gaccttcccg gcctcttccc tgatgagctt tgcgtcatat aatgcatcgc cggcactgaa
     4261 gggagtcagc ctgtccagtt cgtaagagat aacattggcg atattctctt tcaccgtggc
     4321 gggaaattcg gccctcttga ccagtgtcca ggctttgggg atgctcaggg ttatattggc
     4381 cgctgacgct cccagttcac tgagcgccac attcaccgag gaagcgagac cctcggcgct
     4441 tgggtatctg ccatccaaag gatagagcct gaaccctctg attttcatcc tggaaagcat
     4501 ccgtgagcca tagacaacag acagcaggcc cctttcaatg cagaccgtaa cgttctttgc
     4561 aggtgcgatg cgcgcatctg caaggctgag ggagagtgtc ttccagagag gttcccacag
     4621 cgcctttaat cttgctgcag aacttgagac cgcagaggta agcttttcga tggctatggc
     4681 tttggttccg ttcatcggct tgcggtcact ggactcttat agtagagata tttgtatttt
     4741 ttgttgctgt caagggtcac cagggtcacc accgccttga tgggataccc tttttttgcc
     4801 tctcccttgt gcccgatggc ttctacggca aaggcgttgc cgtccgcgat gcttatatat
     4861 gctgcgcttt gggggggaat ccccacttcc tgcagattca ttatcttttt gtcttgcctg
     4921 gaactgatga tggcatccgc aatctcaggg gtaatccccg gaaccgccat caatacctct
     4981 tttggcgctg cgttcacatt gataacgctc attttggaat tgattgtgat aagatccaca
     5041 aggcccttct tcccttcggt tccgaaaaat atctcgtacg acatcccctt caccaggagg
     5101 agttcctcca gggtatcgaa gtttgcgttt tttgccttat aagggttcgg cagggacatg
     5161 tagtagtcgc tttcggcgcc atggagcctg acaaggtcat cagcatcctt ccaatcgaga
     5221 atcgagtcca ctatggtatc ggcgttttca tcctgaacac ccatgtttgt caggagagtt
     5281 tttagtatta taccagaagt ttcattgagg gtgtttaagt ctatcttccc tgactcgtcc
     5341 gtgattctta cctcatattc tccctcaccc aggcgaccgc tgtagggtgt tccgtcggtc
     5401 ttccataatt cactcccttc aaactgtggg agctgggcat tttgcttcat ataaaggatc
     5461 tcctcaatcc ccctctggat tcctgcctcc gcgagaaatt tcatctccat tttttcttta
     5521 aaggcgaggg tggcataggt ttctgtcctc gccataaaag agaaggacag gacgattacc
     5581 atcaggatgg tgagcaccca gagaaccatg aggagggcca cgcccttctg cgacttcatg
     5641 aggggccaca taagaggaat tcgcgagcgc tgtcctttcg tcaatgcaat gctcatttgc
     5701 cctggatctg aggtaccgtt gcagaaggca gctttgccag gtaacctctg gccctcatgg
     5761 gaataaccag ggaaagctca ctgggcccct gtacgagacg cactctcacc ttctcgggga
     5821 tatcggtgtc atctgtccat tgctccaccc agcttccctg ctcatcagtg gggcctttat
     5881 aaaagtactg aaaagagatc tcatccaggg attcaaaaag ttttgcctca atcttatttt
     5941 ccgcaccgat gatactttct gaaatccata gcgtctgttt ccccctggca tcaggaacaa
     6001 cactgtacgt gaccaccaca tatcccatct gcccacccca gagagaatgg ttggagggga
     6061 acagcaaggt ccctttctct cccttaaaat agtatttcct cgagccgtcc tcatcaatgg
     6121 tgagagggat cgccgactga atctgggaat caataagcgt gagggaagcc ctcatccgtt
     6181 ccagggattc gatctttctc tcgcccgcat ccacggagcg gtatcccagt ctcatggctc
     6241 ccgcgacgat gagtacgata aggccgatga gggcgatgga tatcatgagt tccagcaggg
     6301 tgaaccctat tcgggaactc aaatgcggta ttccggaatt cgcaaagaat ctcatcgttc
     6361 gatgacgctc tcttttcgcc ggatcagacc gggtggggac caggtcgggt agtatgcatg
     6421 atgagggctc catatcgtgt gcgccgctat atctgcttgt ttaccagctt cattgtc
//
LOCUS       DATKOU010000236        15812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4560699, whole genome shotgun sequence.
ACCESSION   DATKOU010000236 DATKOU010000000
VERSION     DATKOU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4560699"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VMU21_11200"
     CDS             complement(<1..1775)
                     /locus_tag="VMU21_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUO78129.1"
                     /translation="MSKESLLEGLNPEQREALLYLEGPLLVLACAGSGKTKVIAHKFA
                     HLVKTKKFPYNSILTLTFTNKAAHEMKERISSLTGKDTNGASIGTFHAQCSRLLRKEI
                     QSLGYKPDFTVYDDDDQCTLIRHILREFRFHEALYKGVAAKIGSLKSSLILPEEFLSK
                     GDGFGFDEKLAKVYIRYQDELKRCNALDFDDLIGVTVRLFERHPEILKKYHALFSYIL
                     VDEFQDTNYAQYKLLKLLGSGHNNISVVGDDDQSIYRFRGADVNNVYKFERDFPGAKT
                     IRLEQNYRSTQNILSVASAVISKNPARKPKRLWTGRQGGESVYHCWLNNEDEEARHIA
                     KTIKELYLRGVYEFGDFAVLYRVNIQSRAVEEALRDEGLPYHIVGGISFYHRKEIKDI
                     TAYMRLAINQTDNVSLRRIINCPPRGIGAATLAKIENEAKKKALSLFEVMKSTVKANG
                     VTSAVKERLEGFIKLIERMASKKYKTASDMIQDIIEKSGFEEFVNNDRIQHLNELIAS
                     SAETEVKEFLDKASLFTNMDDSVRGNTVTLMTLHSAKGLEFPVVFILGLEEGVLPYFK
                     ATNEDEINEERRLFYVGMTRAKDIL"
     gene            complement(1873..3699)
                     /gene="glmS"
                     /locus_tag="VMU21_11205"
     CDS             complement(1873..3699)
                     /gene="glmS"
                     /locus_tag="VMU21_11205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HUO78130.1"
                     /translation="MCGIIGYRGKRNAISVVLEGLKRLEYRGYDSAGIAYFSKGHINI
                     KRRKGKVKDLLSLFEADQPSAHTAIGHTRWATHGKPSEENAHPQRSDGIVIVHNGIIE
                     NYLDLKKNLLAEGYVFTSDTDTEVLCHLINKYSKDYLLEDAVRHATKNVKGAYALAVL
                     SEREEGKVVGVKKDSPLCVGLGDGEYFIASDVPAFFNYSKDVIYLNDGEMVVINDDGV
                     MVTTMEGIPVHKEVTSIPWSPSMAEKGGYKHFMLKEMYEQPRAVADTLRGRFSLETGE
                     IYLEEFGLREEDVKSIERIFIVACGTSWHAALIGKYLIEEIARIPVEVDIASEFRYRN
                     PLIVPNDLLIAITQSGETADTLAVQREAKRLGAKVMAICNVVGSSSAREADAVFYTHS
                     GPEIGVASTKAFTSQITALYLFTVALALKKGRTDHDMVMNLLQEILTIPDKIEQILAR
                     NEEMETIAKELFKAKNFLYLGRGIHYPIALEGALKLKEITYIHAEGYPAGEMKHGPIA
                     LIDEEVPVVFLAPKGPLREKVISNIQEVKSRGGKIISLINQGDTDVAQLSHYSISIPD
                     TNSYLTTILLVIPLQLLAYHIGVLRGCDVDQPRNLAKSVTVE"
     gene            complement(3763..5175)
                     /gene="glmU"
                     /locus_tag="VMU21_11210"
     CDS             complement(3763..5175)
                     /gene="glmU"
                     /locus_tag="VMU21_11210"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HUO78131.1"
                     /translation="MNLDCVVLAAGLGTRMKSAIPKVLHTVCGIPMLKAVITAAKTLN
                     PERILVVIGKQGELIKNTIGSHEVSYVLQGEPRGTGHALRCARPALRNSKGVIVVLNG
                     DTPLLKPDTIKKFLHHHKKDKNTISVLSFNARNPEHYGRIIRNASGQVVSIVEHRDAD
                     SGQKEINEVNSGVYAINHDVLDILDKITENPAKGEYYLTDIIAAASRKNLRTSAYCIG
                     TEEEFMGINTREELSRASALMRRNIIQAWIAKGVNFLDAGSVYIHPDVSIGKETTIYP
                     NVCLEGHTKIGKGSVIYPNVRVRNSDIGNGVVIKDSTVIEDSRVKDRASVGPLACLRP
                     GSQVGTAVRIGNFVEIKKSVIGERTKASHLSYIGDAIVGKDVNIGAGTITCNYDGRAK
                     HTTEIEDGVFIGSDTQLVAPVRIGKGAYIGAGSTITRDVPPMSLATSRVCQTNKEGWA
                     QKRQSKAKSEKLKVKKEEKA"
     gene            complement(5172..5786)
                     /locus_tag="VMU21_11215"
     CDS             complement(5172..5786)
                     /locus_tag="VMU21_11215"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HUO78132.1"
                     /translation="MTPIRTILKRRILAGLVVLIPVILTVKVIVWFFMFVDDQLGFMF
                     DKLFDAHIYGLGFLSAVIIVLLVGVVSTNVFGKWTIGVMDNLFLRIPVFKGIYTGLKQ
                     LANAFSPDNTSSFKKFVIVEYPRSGAFAFGFLTKECTLRAEKDHQETYLIAVYIPTNN
                     LYLGEVILFKNQDVFSTDIPIEEGIKIIMSGGIATPSRIQESRV"
     gene            complement(5788..6543)
                     /locus_tag="VMU21_11220"
     CDS             complement(5788..6543)
                     /locus_tag="VMU21_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUO78133.1"
                     /translation="MSGHSKWAQIKHKKAHTDAKRGKTFTKIVKEISVAARIGGGDPS
                     GNPRLRLAIEKAKEVNMPHDNIKRAIMKGTGELPGMSYEEFTYEGYGPGGVAVLIEAL
                     TDNKQRTVSEIRHTLSKHGGNLGEAGCVAWIFQQKGYLLVDKSKVDEDTLMTIALDAG
                     AEDMKNDPKEDNYEVITSPEDLTKVKNALEAANLPVSLAETTMLPKNYVLLEERAAEQ
                     MLKLIELLEENEDVQNVYANFDIPDEVMTKADR"
     gene            complement(6698..8071)
                     /locus_tag="VMU21_11225"
     CDS             complement(6698..8071)
                     /locus_tag="VMU21_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO78134.1"
                     /translation="METILIVEDKDSMARMLQETMETVGYHPLIARDGIEGIRKIREN
                     KIDLVLTDLKLPKKDGMDVLRAAKEENPMLPVIMMTAFGTVDVAVKAIKEGAFDFITK
                     PFDTDHLLHLIRKAMETQRLVTENILLRDEVSSMRGMPTIIGKSEKILDVAKKIQRVA
                     SAKTTVLLLGESGTGKELFARAIHSLSPRKDFPFVPINCAAIPKELLESELFGHEKGA
                     FTGAEAKKLGKFELADKGTIFLDEIGEMALSLQAKIMRIIQEGEIERVGGVKTIPVDV
                     RVIAATNKDIERAVSEGAFREDLYYRLNVFPIRVPPLRERTEDIPLLVDFFTKKYCAE
                     MKTGRKEVSEEVLSLLMDSPWKGNVRELENSIERAVILCDGVTITPEHISLNLIPMNT
                     HLCTLPVLPMDGTLEDTTRVALRLAESERIRKALKETKGNKSRAAELLAVSYKTLLTK
                     IKEYGIE"
     gene            complement(8049..9236)
                     /gene="alaC"
                     /locus_tag="VMU21_11230"
     CDS             complement(8049..9236)
                     /gene="alaC"
                     /locus_tag="VMU21_11230"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HUO78135.1"
                     /translation="MSEFNRIKRLPPYVFAIVNQLKMEARRKGEDIIDLGMGNPDLPT
                     PKHIVDKLCEAAKNPKNHRYSASKGITQLRVAITEWYKRRFDVNLDPETEVVITIGAK
                     EGLSHLAYATLGPGDVVMTPTPAYPIHPYSAILAGAEVRYIPTGKGFDFFEEMEKAYK
                     SSWPKPKLLIINFPHNPTTECIELPFFERIVDFAKENNIMVVHDFAYADLCFDGYLAP
                     SFLQAAGAKDVGVEFFSLTKSYSMAGWRVGFCCGNKEMVGALTKIKSYLDYGMFQPIQ
                     IASIIALRGPQDCVGEIRETYESRRDALIKGLHKAGWNIEPPKATMFVWAEIPEPFKK
                     MGSLEFSKFLIKEACVAVSPGIGFGDGGDAFARFALVENEHRIRQAARGIKKALHGNH
                     PHR"
     gene            complement(9294..10211)
                     /gene="lipA"
                     /locus_tag="VMU21_11235"
     CDS             complement(9294..10211)
                     /gene="lipA"
                     /locus_tag="VMU21_11235"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUO78136.1"
                     /translation="MLRIDDFHHCPSHLDLHRAIRYDAMRLPYWIKTKHFTGLHSTKQ
                     ILRSHGISTVCEQARCPNIGHCFSKPTATFLILGPRCTRQCGFCSVDSASLPLDPPSA
                     DEPGRVARTAQEMGLKYVVITSVTRDDLPDGGALHFAHTIKALKDVLPDAGIEVLIPD
                     FKGDIGALKIVLHSEPDVVNHNVETVPRLYSLVRPQADYGLSLYVLKMAKKQAPHIRI
                     KSGVMVGLGETFEEIVSVMKDLSSAGCDFLTIGQYLRPSKRNLPVVEYVAPGTFIDYK
                     ETALRMGFKGVASSPLVRSSMNAEEMFLS"
     gene            complement(10238..10420)
                     /locus_tag="VMU21_11240"
     CDS             complement(10238..10420)
                     /locus_tag="VMU21_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78137.1"
                     /translation="MAKHAGAYKSEKRRKEVSRQKKREEKLQRRLHKGLDTNPEGEQG
                     LAQSEVSQDEKTSDNA"
     gene            10669..10869
                     /locus_tag="VMU21_11245"
     CDS             10669..10869
                     /locus_tag="VMU21_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HUO78138.1"
                     /translation="MSNGTVKWFNESKGFGFITAEDGSDVFVHYSSIQGDGFKSLAEG
                     DAVSFDIENGPKGPKAINVVKG"
     gene            10935..11321
                     /locus_tag="VMU21_11250"
     CDS             10935..11321
                     /locus_tag="VMU21_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78139.1"
                     /translation="MVHKQNYSARRELNRQNRLATGLLSERFPNVSEIVIHMTYYQKA
                     VNQLLMLRTVHVLPTDSAYFNMECVIKGCIEGGFDLTSVISSMVKTHKKVGKGALVCC
                     GKTDTLAHDHASISYEIEIQYHKKSR"
     gene            11524..12258
                     /locus_tag="VMU21_11255"
     CDS             11524..12258
                     /locus_tag="VMU21_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO78140.1"
                     /translation="MSAWATGKGQTLLQPIFLIEDDTKIARVAKVYLEGAGYRVVHFE
                     KGKDAVNFALNEKPLAVILDLMLPDTSGETVCQELKEIGDFPIIMLTSKSSEEERVAG
                     FALGADDYVVKPFSPRELVYRVKAVLKRAQKKDLTAAEPMNFNGGLLVIDGHTYTVKK
                     KGLLLNLTPTEFKVLYTIASYPGKVFTREELVEKALGYQFEGYERSIDAHIKNIRHKI
                     EDDPKNPSLILTIYGVGYRFSGKRDV"
     gene            12251..13597
                     /locus_tag="VMU21_11260"
     CDS             12251..13597
                     /locus_tag="VMU21_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78141.1"
                     /translation="MFKNLWVKFFLLFLVVSVVALSSALIMRELMVQDFRGFLEGQVE
                     DRAYWITASLESTYEKYGVWANPEIIENTIWALMLGFDMRLHDAEGNSIIDTEGATNT
                     LSSFVRKRIEAISDLRVKDTQDKYQPYALFYAGKEIGRVELKFLRSKKEMTFITRSNR
                     LLFLTLLALGGFAVLLSVIFARRLTRPIHGLTKGVTAIAEGNLRSRVVMSGEDEISRL
                     SAAFNRMAQTLEIQESLKKKLTSHIAHELRTPLSAIRGELEGMIDGFIAMDKDHLQSL
                     YAEIGRLRSILEGIEELSQAEASSLTLNKQIFELRPFLSNIVGRFSRIFQDKGVAIQL
                     QCDDRLRVCADPDKLSQIVINLVSNSLKATDKGGSVRIDATANESEVIIEVIDNGCGI
                     TQEDLPLIFERFYKASEGGLGLGLAIVKELVEAHAGKIEVHSEYRKGATFTLSLPL"
     gene            13681..14016
                     /locus_tag="VMU21_11265"
     CDS             13681..14016
                     /locus_tag="VMU21_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUO78142.1"
                     /translation="MKGGEERMKVLTILLTVAIAVVLIGSAIAVPPGKTVEFAGGPAG
                     KVVFDGKIHADKGLKCNDCHTKIFQMKKSATMKMADMNAGKFCGECHNGTKAFATKDA
                     ANCGKCHKK"
     gene            14232..14552
                     /locus_tag="VMU21_11270"
     CDS             14232..14552
                     /locus_tag="VMU21_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78143.1"
                     /translation="MKRQILMSLVIGLIFLYASAASKAFGADESGPYGGSREGGYGEK
                     KEVLTPGDAERILRQYFAKKNVTIGEIRERELYFEAEVRDKDNKVIDKVIVDKRTGRI
                     RSIY"
     gene            complement(14567..15745)
                     /gene="larC"
                     /locus_tag="VMU21_11275"
     CDS             complement(14567..15745)
                     /gene="larC"
                     /locus_tag="VMU21_11275"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HUO78144.1"
                     /translation="MKIAYFDCSSGISGDMCLGALVDAGLPLKEIQKGLKALPVKGYK
                     LETRKVKRAGIAVTKVDVVIRQSAISGQQPAKRWRDIKKIIKYSSLPDGIKQRGLSIF
                     RRLFEAEGRVHGNEYTRTHLHELGAVDCIVDIFGTLIGLNILEVGKVYSSALNLGMGS
                     VITEHGRLPVPAPATAEILKGVPVYSSDVPVELTTPTGAALVRELAQNYGHIPMMKIE
                     HTGYGAGQKDIPGLPNTLRIFFGEETVLENAEPHPRVLVIETNIDDMNPQCYEYVMDR
                     LFSSGALDVYLTQVIMKKGRPGVLLTVLCGEDRKEEIIDLLFKETTTIGLRFYKVARR
                     ILEREIRRVKTESGTIRVKVSKGLDGTLKFSPEYEDCKGIAEQHKIPLIEVMKRAKPF
                     "
BASE COUNT         3859 a         3815 c         3710 g         4428 t
ORIGIN      
        1 cagaggatat cctttgccct ggtcatcccg acataaaaaa gccggcgctc ttcgttgatt
       61 tcgtcttcat tggtggcttt gaagtaaggc agcacacctt cctcaaggcc gagaataaac
      121 accacaggga attcgagccc ctttgcactg tggagggtca tgagggtgac ggtattccct
      181 ctgaccgaat cgtccatatt ggtgaaaaga gacgccttgt caaggaactc ctttacctcg
      241 gtttccgcag aggaagcgat gagttcattg agatgttgta tcctgtcatt attgacaaat
      301 tcctcaaagc ctgatttctc aatgatatcc tgtatcatgt ccgaggcggt cttatatttc
      361 ttggatgcca tacgttcgat gagtttgata aaaccctcga gcctttcctt tactgccgag
      421 gtgaccccgt ttgctttcac agtcgacttc atcacttcga agagacttaa tgctttcttc
      481 tttgcctcat tctcaatctt ggcgagcgtg gcagcaccaa ttcccctggg agggcaattt
      541 attatcctcc gcaggctcac attatcagtt tgattgatgg cgagacgcat atatgcggta
      601 atatccttta tctccttcct gtgatagaaa cttattccac ccacaatgtg ataggggagc
      661 ccctcatccc tcaatgcctc ttccacggcc cttgattgta tgtttaccct gtagagtact
      721 gcaaaatcgc caaactcgta tacgcccctt agatatagct ccttgatcgt cttggcaata
      781 tgacgagctt cttcgtcttc gttgttcagc cagcaatggt agacactctc ccccccttgc
      841 cgtcctgtcc agagtctctt gggttttctc gcaggattct ttgagatgac cgcactcgca
      901 acgctcaata tgttctgtgt ggagcggtaa ttttgttcga gacggatggt ctttgctcca
      961 ggaaagtctc tctcaaactt atatacgtta ttgacgtcag cacctctgaa tcgataaatg
     1021 ctctggtcat catcacccac cactgagata ttattatggc ctgacccgag cagtttcaaa
     1081 agcttatact gagcatagtt tgtgtcctgg aattcatcaa caagtatgta agagaagagg
     1141 gcatgatatt ttttaaggat ttccgggtgt cgttcaaaaa gccttacggt gactccgatg
     1201 agatcgtcaa agtcaagggc attacatctc ttcaattcat cctggtaccg tatataaacc
     1261 tttgcaagtt tttcatcaaa cccgaagccg tcacctttgg aaaggaactc ctccggcaag
     1321 atcagagaag acttgaggga gcctatcttg gctgccaccc ctttatagag agcctcgtga
     1381 aatctgaatt ctctcaaaat gtgccggatg agggtgcact ggtcgtcatc gtcatagact
     1441 gtaaaatcgg gtttgtaccc gagggattgt atctcttttc tgagaagcct gctgcattgc
     1501 gcatggaatg tcccaataga tgcaccgttc gtgtcctttc cggtgaggga ggatattctc
     1561 tccttcattt cgtgagcagc cttattggtg aaggtgaggg tgagtatgga attgtagggg
     1621 aacttcttcg ttttcacaag gtgcgcgaac ttgtgggcaa tgacctttgt cttgccgctc
     1681 cccgcgcagg caagcaccag cagaggaccc tctaaataga gaagtgcttc acgctgctca
     1741 ggattaaggc cttccaataa actttccttt gacatctcgg ctccttttat actagcaaaa
     1801 caagggcgtg tgcgtcaaaa acagaacccg gaagagagag cagaacaatg ctctgtgttc
     1861 cggccctaag tcttactcga ctgtgacact ttttgccaaa tttcttggtt gatcgacatc
     1921 acagcccctc aatacgccta tatgataagc aagaagttgc agaggaatga cgagcagaat
     1981 agtggtaaga tacgaattcg tgtcgggtat ggaaattgag tagtgagaga gttgtgcgac
     2041 gtccgtatcc ccttgattaa tcagcgagat aatctttccc cccctgcttt taacctcctg
     2101 tatattggaa atgacttttt cacggagtgg tccttttggt gccaggaaga caacaggaac
     2161 ttcctcatca atgagtgcga tgggaccatg tttcatctct cctgcaggat atccttccgc
     2221 atgaatgtaa gtaatctctt tgagcttcaa cgctccttca agggcaatgg gatagtggat
     2281 ccccctgcca aggtagagaa aattctttgc cttgaatagc tcttttgcga tagtctccat
     2341 ctcctcgtta cgtgcaagga tctgctcgat tttgtcagga atggtgagaa tctcctgtag
     2401 gagattcatc accatgtcat ggtcggtccg ccctttcttg agtgcgagtg caacggtgaa
     2461 cagatagagc gccgttatct gggaagtgaa tgccttggtg gatgccaccc ctatctcggg
     2521 gcctgagtga gtataaaaga ccgcatctgc ttcccttgcg gaggaactgc cgaccacatt
     2581 gcatattgcc atgacctttg cgccaagtcg tttggcttcc ctctgtactg caagggtgtc
     2641 agccgtctcc cctgattggg taatggcgat gaggaggtca ttgggcacta tcaggggatt
     2701 tctgtacctg aactctgatg cgatatccac ctccacaggg attcgggcaa tttcttcaat
     2761 aaggtacttt ccaatcaggg ccgcatgcca tgaggtgccg catgccacaa tgaatatcct
     2821 ttcaatggat ttcacatcct cttccctaag accgaattcc tccagatata tctcgccggt
     2881 ttcaagacta aaccttcccc tgagcgtatc tgcgacagcc cttggttgtt cgtacatctc
     2941 tttaagcata aaatgcttgt atccgccctt ttccgccatg gacggactcc atggaattga
     3001 cgtcacctcc ttgtgaaccg ggatcccctc cattgtggtc accatgacgc cgtcatcatt
     3061 tatcaccacc atctcgccat cattaagata gatcacatct ttggaataat tgaagaatgc
     3121 ggggacatcg gatgcgataa aatattctcc gtccccgagt cctacgcaaa gcggactgtc
     3181 ctttttcaca cccacaacct tgccttcttc tctttcgctg agtaccgcca gtgcatatgc
     3241 acccttcaca ttttttgtgg catgcctcac cgcatcctcc aggagatagt ccttggaata
     3301 tttattgatg aggtggcaca gcacctctgt atcggtatct gaggtaaaga cgtatccctc
     3361 ggccagaagg ttcttcttaa ggtcgaggta gttttcgata ataccgttat ggacaataac
     3421 gatgccatca gacctctgag gatgtgcgtt ttcttctgat ggtttaccat gggtcgccca
     3481 tcgagtgtgg ccaatagcgg tatgggctga tggctggtcc gcctcaaaga gactgaggag
     3541 gtcctttacc ttgcctttgc gtctcttgat attaatatgc cccttggaaa agtatgcgat
     3601 ccctgctgaa tcgtagcccc tgtattccag gcgtttgagc ccttcgagga ccacagatat
     3661 ggcatttctt ttccctctat acccgattat tccgcacatt ttacagtgac cagtgaatag
     3721 tgattagtga atagtatcct gcacttgacg cttactttca tactatgctt tttcttcttt
     3781 cttaactttc aacttttcac ttttagcttt tgactgccgt ttttgcgccc atccctcctt
     3841 gtttgtttga cataccctgc tcgtggcaag ggacatggga ggcacatcac gtgtgatggt
     3901 agagcctgcg ccaatatatg cgcctttgcc aatacgcacg ggagcgacaa gttgcgtatc
     3961 gctgccgatg aacacgccat cttctatctc ggtggtatgc tttgccctcc cgtcataatt
     4021 acaggtgatg gtgccggccc cgatattcac gtccttcccc acaatggcat ccccgatata
     4081 gctaagatgg gatgcctttg ttctttcacc tatgaccgat ttctttattt caacaaagtt
     4141 gcctattctg acggcagtcc ctacttgaga accgggtctt agacaagcaa gagggcccac
     4201 agatgcccgg tcctttaccc tggagtcttc tattacggtg gagtctttta ttactacacc
     4261 atttccgata tcactgttcc gaaccctcac attcggataa atgacggagc cttttcctat
     4321 tttcgtatgt ccctcgagac agacgttcgg atagattgtc gtctcctttc cgatggatac
     4381 gtcgggatgg atatataccg agccagcatc aagaaaattt acgcccttgg caatccaggc
     4441 ctgtataatg ttcctcctca tgagcgctga cgccctggaa agttcttccc ttgtatttat
     4501 ccccataaat tcttcttctg tgcctatgca ataggcggag gttcttaggt tcttccttga
     4561 ggcggcggca attatatccg tgaggtagta ttcacccttc gccggattct ccgttatctt
     4621 gtcaaggata tcaaggacat catggtttat ggcatacaca ccgctgttta cttcattgat
     4681 ttctttctga ccggagtctg catccctgtg ctccacgatg gacacgactt gtcctgaagc
     4741 atttctaata atcctgccat agtgttcggg attccttgca ttaaatgaca atacggagat
     4801 ggtgttctta tcttttttgt ggtgatgcag gaattttttt atggtatcgg gcttcaaaag
     4861 aggggtgtct ccgttaagca ccacgatgac acctttagaa ttcctgagag caggtctcgc
     4921 acagcggagg gcatggccgg tgccccttgg ttctccctgg agcacatacg acacctcatg
     4981 ggatcctatt gtgttcttga tgagttctcc ctgtttccca atcaccacga gaatcctttc
     5041 agggtttagt gtctttgctg cagtaataac cgccttgagc atcgggattc cgcataccgt
     5101 gtggagcacc tttggaatgg ctgacttcat tcttgttcca agccctgcgg caaggacaac
     5161 gcagtccaga ttcataccct tgattcctgt atcctcgacg gtgtcgcaat ccctcccgac
     5221 atgatgatct tgatgccttc ttcgatcggt atgtcggtgg agaacacgtc ctggtttttg
     5281 aagaggatta cttcacccag gtagaggttg tttgtgggaa tatacaccgc gataagatac
     5341 gtttcttggt ggtctttctc tgctctcagt gtgcattcct ttgtgaggaa cccgaaggca
     5401 aaagcgccgg accttgggta ttcaacaata acaaacttct taaatgaact ggtgttatcg
     5461 ggtgaaaatg cgttggcgag ctgcttcaat ccggtgtaaa ttcccttgaa cacggggatc
     5521 ctgaggaaca ggttgtccat gacccctatc gtccactttc cgaagacatt cgtcgagact
     5581 acaccgacta agagaacaat aattaccgca gagaggaacc cgagtccgta aatatgggcg
     5641 tcgaacagtt tgtcaaacat gaaaccgagc tggtcatcca caaacatgaa aaaccagaca
     5701 atcaccttga cggtgagtat gacgggaatt aatacgacaa gccctgcaag gattcttctt
     5761 tttaggatgg tacggatggg ggtcatctta cctgtcagcc tttgtcataa cctcgtcagg
     5821 aatatcgaaa ttggcgtata cgttctggac atcttcgttt tcctcgagga gttctatgag
     5881 tttcagcatc tgctccgcag ccctttcctc aaggaggaca taattctttg gaagcattgt
     5941 cgtttctgca agggaaacgg gcaaattcgc agcttcaaga gcgttcttca ccttggtgag
     6001 gtcttcgggt gaggtgatga cttcataatt gtcttccttc ggatcatttt tcatgtcctc
     6061 agcaccggcg tcaagggcaa tagtcatgag ggtatcctcg tcaaccttgc tcttatccac
     6121 gaggagatat cctttttgct gaaatatcca tgctacgcac cccgcttccc ctaagttccc
     6181 cccgtgtttt gacagggtgt gccgaatctc cgagacggtt ctctgtttat tatcagtgag
     6241 ggcctcgatg agtaccgcaa cgcctcccgg cccgtacccc tcataggtga actcctcata
     6301 tgacatacca gggagttccc ccgtaccctt cataatcgcc cttttgatgt tatcgtgggg
     6361 catattcacc tcttttgcct tttcgatggc aagccggagt cttggattac cggaggggtc
     6421 acccccgccg atccttgccg caacagagat ctccttcacg atcttggtaa atgtttttcc
     6481 tctctttgca tcggtatggg ctttcttatg ctttatctgg gcccatttag aatgtcctga
     6541 cattcatgat cctccgtcac tttctgacaa atctcaaata aacatcttag gtcacttaag
     6601 aaaatttgca gaagtggcac tccttagtat cgaatttcca tccacaaagt cgcattttgc
     6661 gaggcattta ttttagcata gaagcctccc ctttgtgtta ctcaatgcca tactctttta
     6721 tcttggtcaa gagggttttg tagcttactg caagaagttc cgcagccctg ctcttgttac
     6781 cctttgtttc ctttaatgcc ttccttattc tttcgctctc tgccaggcga agcgccactc
     6841 ttgtagtatc ttcgagggtg ccgtccatgg gtaacacggg taacgtgcaa aggtgtgtat
     6901 tcatgggaat gaggttcaag gagatatgtt caggggttat agtaacgccg tcacagagga
     6961 ttaccgctcg ttctatagaa ttttccagtt cccttacgtt ccctttccat ggagagtcca
     7021 tgagcagaga caacacttcc tcagagacct cttttcttcc tgtcttcatc tcggcgcagt
     7081 atttttttgt gaagaaatcc actagcaggg ggatatcctc tgtcctttcc cttagcggag
     7141 gtacccttat gggaaagaca ttaagtctgt agtagaggtc ttccctgaat gccccttccg
     7201 aaacagccct ttcgatgtct ttattggtag cagcgatgac ccgcacatcc accggtatgg
     7261 tctttactcc ccccacacgc tcaatttctc cctcctgaat gatcctcatg atcttcgcct
     7321 gaagggaaag ggccatctca ccaatctcgt cgaggaatat ggttccctta tctgccaatt
     7381 cgaacttgcc gagttttttt gcctccgccc cagtgaaagc ccctttctca tgtccaaaaa
     7441 gttctgattc caggagttcc tttggtatgg cagcgcagtt tatggggaca aaggggaagt
     7501 cttttcttgg gctgagagag tgtatggccc ttgcgaacaa ctccttgcca gttccacttt
     7561 ctcccagaag aaggaccgta gtttttgctg atgcaaccct ttggattttc tttgcaacat
     7621 caaggatctt ttcggacttt ccaatgatcg ttggcatgcc tctcattgac gagacctcat
     7681 ccctgagaag gatattctct gtcaccagtc tttgggtctc catagctttt cttataagat
     7741 ggagaagatg gtctgtgtca aagggttttg tgatgaagtc gaatgcaccc tcttttatcg
     7801 cttttacggc tacgtctacg gtaccaaagg cggtcatcat gattacaggc agcatagggt
     7861 tttcttcttt tgcggccctg agcacgtcca tgccatcctt tttcggaagc tttaggtctg
     7921 tgaggacaag gtctattttg ttctccctga tctttctgat gccctctatt ccatccctgg
     7981 caataagtgg atggtacccc acggtttcca tggtctcctg caacatcctg gccatggagt
     8041 ctttgtcttc aacgatgagg atggtttcca tggagtgcct tttttattcc cctggcagcc
     8101 tgcctgatcc tgtgttcgtt ttccacaagg gcaaatctcg caaaggcatc tccgccatct
     8161 ccgaacccga tcccaggtga aacggcgacg catgcctcct tgatgaggaa tttgctgaac
     8221 tcgagagagc ccatcttctt gaaaggttcc ggaatctcgg cccacacaaa catggttgcc
     8281 ttgggtggtt ctatattcca gcctgcctta tgaaggcctt ttatgagcgc atccctccgg
     8341 gattcgtacg tttctctgat ctcccccacg caatcctggg gacccctcaa ggcaataatg
     8401 cttgcgatct gtattggctg gaacatgcca taatccagat aactctttat ctttgtgagc
     8461 gcgcctacca tttctttgtt tccgcagcag aaccccactc tccatcctgc catagaataa
     8521 ctctttgtca gcgagaagaa ttcaactccc acgtctttgg cgccggcagc ctgaagaaaa
     8581 ctgggagcaa ggtatccgtc aaaacagagg tcggcatacg caaaatcgtg gactaccatg
     8641 atgttgtttt ctttcgcaaa gtccacgatc cgctcgaaaa aggggagttc aatacactcg
     8701 gtggtgggat tatgaggaaa atttatgatg agcagcttag gtttcggcca tgatgacttg
     8761 tacgcttttt ccatttcttc aaaaaaatca aacccttttc ccgtaggtat gtacctcacc
     8821 tcagcacctg cgagtatggc gctgtaaggg tgaataggat atgcgggagt gggtgtcatc
     8881 accacgtcac caggtcccag cgtcgcataa gcgagatggc tcaatccctc ttttgctcca
     8941 atggtgatca ccacttctgt ttcagggtcc aggttgacat caaaacgcct cttgtaccat
     9001 tcggttatag ccactctcaa ttgggttatc ccctttgacg ccgaataacg gtgatttttc
     9061 gggtttttgg cagcctcgca tagtttatcc acgatatgtt ttggtgtggg gagatcggga
     9121 ttccccatgc cgagatctat gatatcctcg ccttttctcc tcgcctccat cttcagctgg
     9181 tttactatgg caaatacata agggggaagc cgtttgattc tgttgaactc tgacatccag
     9241 aactcctttt ctaatatgtt cttatatggt atgtgtcaat taatataaca aattcaagac
     9301 aaaaacatct cttctgcgtt catggaactt ctcaccaatg gagaggaagc aaccccctta
     9361 aagcccattc ttaaggcggt ttctttgtaa tcgataaaag tgccgggtgc aacatactct
     9421 acaacaggga gatttctttt tgagggtctc aggtactggc ctatggtgag aaaatcgcat
     9481 cctgctgagg aaaggtcctt cattaccgaa acaatctctt caaaagtctc accaagaccc
     9541 accatgacac ctgattttat gcgaatgtgg ggcgcctgct ttttagccat tttcaggaca
     9601 tataatgaga gcccatagtc tgcctgtggt ctcactaggg aataaagcct tggcactgtc
     9661 tcgacattgt gattcactac atctggctca gaatgcagga ctatcttcaa cgcccctatg
     9721 tctcccttga agtcagggat caagacctct attcctgcgt caggcaggac atcttttaat
     9781 gcttttatcg tgtgcgcaaa gtgcagggct cccccgtccg ggagatcatc cctggtgacc
     9841 gatgtgatca ccacgtactt caggcccatc tcctgtgcag tacgcgcgac cctcccaggt
     9901 tcgtctgctg agggtggatc aaggggaaga gatgcagagt ccactgaaca aaaaccacat
     9961 tgccttgtac agcgagggcc taggataagg aaggtggctg taggtttcga gaagcagtgc
    10021 ccgatgttgg gacatctggc ttgctcgcag accgtggaga ttccatggct tctcaatatc
    10081 tgtttggtgg agtgaagtcc ggtaaagtgc tttgtcttta tccaataggg gagcctcatg
    10141 gcgtcatatc tgattgctcg gtgcagatcc aggtgactgg gacagtgatg aaaatcatct
    10201 atacgcaaca agtcatgcca gctgctgcgt gctcacacta agcattgtca ctcgtctttt
    10261 catcttgcga aacttcgctt tgggcaagtc cctgttcacc ttccgggttg gtgtcgagcc
    10321 ccttgtgaag tctcctctgg agtttttctt cccgtttttt ctgacgggac acctcttttc
    10381 tccgcttttc gcttttgtat gctcccgcat gttttgccaa tgtagccctc cttcatgttg
    10441 gatagaacat tatatcatgg aggagaatgg gaatgctcaa gatcccatag atcacccgtg
    10501 cgacggcagg gaaaggttga ctttttattc acaaacatgt aaccataaaa atgagtttca
    10561 gaagtttttg taactgataa ggccacaaag acttttagct ttgtggcgtt ttttgttgaa
    10621 gtaggtggtc actccggaac ttagtttata cgtgaggagg tacgagaaat gtcgaatggg
    10681 acagtgaagt ggttcaatga atccaaaggg ttcggcttta tcactgctga agacggcagt
    10741 gatgtatttg tccactactc ttccatccag ggcgacggtt tcaaatctct tgccgagggt
    10801 gatgcggtga gctttgatat tgaaaatggg ccaaaaggcc ccaaggccat taatgtagtg
    10861 aaaggttaat aaccaagaaa gtccctccgc gcgcgaaggg actttttcct atcaggggaa
    10921 gagacacaga gaaaatggtg cataaacaga actattcggc caggagggaa ctaaacagac
    10981 agaaccggct tgcgaccgga cttctttctg aacggtttcc caatgtttct gagatagtaa
    11041 ttcatatgac atactatcaa aaggctgtaa atcagcttct catgctgcgt actgtgcatg
    11101 tccttcccac agattccgca tatttcaata tggaatgtgt gataaagggc tgtatcgaag
    11161 gtggctttga ccttacttct gtcatttcgt ccatggtgaa gacccataag aaggtgggaa
    11221 aaggagccct ggtttgttgt ggaaaaaccg atacccttgc tcatgatcac gcgagtatct
    11281 cttatgaaat cgagatacaa tatcacaaaa aatcccgata ggaaatttac ttatcctcct
    11341 cgccacgagt caacccttac actagaacaa aagacccctt agagcttccg gccctccgca
    11401 atttgcagta tactagaggc aaccgaagag caatattgtg catgagagat gatgcgagcg
    11461 gtgcattagg aaggcaaaag catgacgcgg agtgcttgaa ggcgctcaat aacctaaaat
    11521 agaatgagcg cgtgggcgac agggaaaggg cagacattgc ttcaaccaat ttttctcatt
    11581 gaagacgaca caaaaattgc cagggtagca aaggtctatc tcgaaggcgc ggggtacaga
    11641 gtcgtccatt tcgaaaaagg gaaagacgct gtgaattttg cacttaacga gaagcctctt
    11701 gcagttatcc ttgacctcat gctgccggat accagtggtg aaactgtatg tcaggaacta
    11761 aaggaaatag gggatttccc catcattatg ctcacttcaa aatcctcaga agaagagagg
    11821 gtggcagggt ttgcccttgg cgccgatgat tatgtggtaa agcctttcag tccgagggaa
    11881 ttggtctaca gggttaaggc agtgctcaaa agggcgcaga agaaggatct gaccgctgcg
    11941 gagcccatga atttcaatgg cggcctcctt gtcattgacg gccacacgta tactgtgaag
    12001 aagaaagggc tcttgttgaa tcttacgcca acggaattca aggtgctcta taccattgca
    12061 tcctatccgg ggaaagtctt tacaagagag gaactcgtgg aaaaagccct cggttatcag
    12121 tttgaaggat atgagagaag cattgatgcc cacatcaaga atatcaggca caaaattgag
    12181 gatgatccca aaaacccttc tctcatactc accatttatg gtgtgggcta tcgtttctca
    12241 ggaaagaggg atgtttaaga acctctgggt aaaattcttt cttcttttcc tggttgtttc
    12301 tgttgttgcc ttatcctcag ccctgattat gagagaactt atggtgcaag acttcagggg
    12361 gttcctcgaa ggccaggttg aggacagggc atactggata acagcctccc ttgaaagtac
    12421 atatgaaaag tacggggtgt gggcaaaccc ggagattatc gaaaacacga tctgggctct
    12481 catgctcggc ttcgacatga gacttcatga cgctgagggt aattctatca ttgacacaga
    12541 gggcgctacc aatacgcttt cgtcgttcgt gagaaagagg attgaggcaa tttcagatct
    12601 gagggtcaag gatacccagg acaaatacca gccgtatgcg ctcttctatg ccggtaagga
    12661 gatagggaga gttgaattga aattcctgag atctaagaag gagatgacct tcataacccg
    12721 ttcgaacagg ctgctctttc tcacgttgct cgcactggga ggttttgcag ttctcctgag
    12781 cgtcatcttt gcgagaaggc tgacaagacc cattcatggt cttaccaagg gagttaccgc
    12841 gatcgccgag ggaaacctca ggagcagggt ggtcatgagc ggtgaagacg aaataagcag
    12901 gctgtcagct gcgtttaaca gaatggcaca gaccctcgaa atacaggagt ccctcaagaa
    12961 aaaactcact tcacacatag cccatgaact cagaacgccc ctcagtgcaa tacggggaga
    13021 acttgagggg atgattgacg gatttatcgc catggacaag gatcatctcc agtccctcta
    13081 cgcagagatc ggcagactca gaagtatcct tgagggaatt gaggaacttt cccaggctga
    13141 ggcaagtagt ctcaccctca ataaacagat ttttgagctc agacctttcc ttagcaatat
    13201 tgtcgggaga ttctccagaa tctttcagga taagggtgtc gccattcaac tgcaatgcga
    13261 tgaccgtctc agggtttgtg cagatcctga caagctgagc cagatagtca ttaacctcgt
    13321 gagcaattca ctgaaagcca cggataaagg ggggagcgta cgtattgacg ccactgcaaa
    13381 cgaatcagag gtaataatag aggtcattga caacgggtgc ggcataacgc aggaggacct
    13441 tcccctcatc tttgaaaggt tctacaaggc ttctgaggga gggcttggtc tcgggcttgc
    13501 tattgtaaag gaactcgtgg aggctcatgc cggaaagata gaggtacaca gcgaatatag
    13561 aaaaggggcg acctttaccc tttcactccc tctgtaacca ttcacaattc ttcataatcc
    13621 tttcgctgga cgttcacaat tctttgctaa gatgtgagca atgaaaagag aaagaactac
    13681 atgaaaggag gtgaagagag aatgaaggtg ttgaccattt tattgacagt agcaattgcg
    13741 gtggtgctta tcggcagcgc aattgcggtg cctccgggaa agactgttga gtttgcggga
    13801 ggaccagcag gcaaggtcgt atttgatggc aagatccatg cagataaggg gcttaaatgc
    13861 aatgactgcc atacgaagat attccagatg aagaagtctg cgaccatgaa gatggcggat
    13921 atgaacgctg gcaagttctg cggggaatgc cataatggta caaaggcatt tgcaacaaag
    13981 gatgcggcga attgtggaaa gtgccacaaa aagtaattga gaaagcaaca aacagggcga
    14041 ctcttaaagg tcgccttttt tgttgcacaa gaaagataaa ataaacccat aaattaaata
    14101 aagaaccccc tacctacgtg ggaaagttgt caacttccag aattcattga cccgtccagc
    14161 ggtaacctct ataatgttct ctaaggagag tgaaccttat tgccggcatt tcgggaaagc
    14221 gagggaaagt gatgaagagg cagattttga tgagccttgt tataggactg atttttcttt
    14281 atgcaagtgc cgcttctaaa gcttttgggg ctgatgagag tggtccctac ggaggttcac
    14341 gggagggggg atatggggag aaaaaagagg tcttgactcc tggcgatgca gaaagaatac
    14401 tgaggcaata cttcgcaaaa aaaaatgtga cgatagggga gatcagggaa agagagctct
    14461 actttgaggc tgaggtgagg gataaggata ataaggtaat tgacaaagtg attgtggata
    14521 aaaggaccgg cagaataagg tcaatatact gaggccgagc aggaacctaa aaaggttttg
    14581 cgcgtttcat cacttcaata agaggtatct tatgttgttc tgcaattccc ttgcagtctt
    14641 catattcagg ggagaacttg agagttccat caagcccctt cgataccttt acccttattg
    14701 taccggattc ggttttcacc cttcgtatct cacgttcaag gatccttcgt gccactttat
    14761 aaaatcgcaa accaatggtg gtggtctcct tgaagaggag atcgatgatc tcctctttcc
    14821 tgtcttcccc gcagagtaca gtaaggagca caccggggcg cccctttttc atgattacct
    14881 gggtgagata cacatcaagg gcgcctgaac tgaaaagcct gtccataacg tattcgtagc
    14941 attggggatt catgtcatct atattggtct ctataaccaa aacccttggg tgaggttcag
    15001 cgttttcaag tactgtttcc tctccaaaga aaattctgag agtgttcggc aatccgggta
    15061 tatccttctg tcctgcacca taacctgtgt gttcaatctt catcatgggg atatgcccat
    15121 aattttgtgc taattctctc actagggcag cacctgtggg ggtggtgagt tcaacgggga
    15181 catctgatga atagacaggg actcccttga ggatttctgc tgttgccggt gcagggacag
    15241 ggagtcttcc atgttccgtt atgaccgaac ccattccaag attgagcgct gatgaataca
    15301 ccttcccgac ttccagaata ttgagaccga tcagggttcc aaagatgtcc acaatgcagt
    15361 ccactgcgcc aagctcatgg agatgcgttc tggtatactc attgccatga acccttccct
    15421 ctgcctcaaa aagccgtctg aagatgctga gccccctctg tttaattcca tcagggagcg
    15481 atgagtattt tattattttc ttgatgtctc tccatctttt tgcgggctgc tgaccgctga
    15541 ttgctgactg ccggatcacg acatcaacct ttgtaacagc aatcccggct ctcttcacct
    15601 ttcttgtttc gagcttatat cccttgacag gaagagcttt gagtcctttt tgtatctctt
    15661 ttaagggcag acctgcatcc acaagggccc cgaggcacat gtcgccgctt atgcctgatg
    15721 aacagtcaaa atacgcaatc ttcaaattat cctcccatca ctgtcatata atacaaaaca
    15781 ttgcgagttt catccagagt tcatccttga tc
//
LOCUS       DATKOU010000237         7883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1879005, whole genome shotgun sequence.
ACCESSION   DATKOU010000237 DATKOU010000000
VERSION     DATKOU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879005"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VMU21_11280"
     CDS             <1..752
                     /locus_tag="VMU21_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HUO78145.1"
                     /translation="VMMLRAGRAVGGRMPVVISSEAGGTMIHEAIGHGLEADLAQHGL
                     SVYSNKLGQEVASRLVTVIDDATLPHKRGSYLFDDEGTPSRRTVLVDKGILSGYLYDQ
                     LSAMHDGVKSTGNGRRESYQHRPIPRMSNTFIAPGEASPEAVVKSTWNGLFVKKMGGG
                     QVNTVTGDFVFDVQEGYTIESGTIGEPVREATLTGNGPSILRSIDMIASDLGFSIGTC
                     GKDSQGVPVSDAMPTLRISEMVVGGEISRSR"
     gene            899..1783
                     /gene="lpxC"
                     /locus_tag="VMU21_11285"
     CDS             899..1783
                     /gene="lpxC"
                     /locus_tag="VMU21_11285"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HUO78146.1"
                     /translation="MRLQRTLRQEVTFSGIGLHTGRHAVVRLKPAPRDSGIIFLRIDR
                     MAVIRAHIGAVTDTAFATTLGNDNIKIRTVEHLLAALAGIGIDNLIIEVDGPEIPILD
                     GSSTELVSIILQGGIAKQGKKMPCLRITKPIIMEDGHSEIAVVPYEGRRITFRIHFPH
                     HFLGEQELSIELNEETFIKEVAPARTFGFLKDIQYMMANGLAKGGSLNNAVILSDKGV
                     LNSSGLRFKDEFVRHKILDSIGDFSLIGFPIYGHIFAHKAGHSTNLKFIKKVISCSDC
                     WKIVSEAEQPQAQAQLHL"
     gene            complement(1798..2007)
                     /locus_tag="VMU21_11290"
     CDS             complement(1798..2007)
                     /locus_tag="VMU21_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78147.1"
                     /translation="MKNNGDKEGSSEETRSEEDSEEDRKEEIRKVLWDLGLEKGFSSL
                     FFLFAVFLRYETPFSEKIQDKFVTS"
     gene            complement(2044..2598)
                     /locus_tag="VMU21_11295"
     CDS             complement(2044..2598)
                     /locus_tag="VMU21_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HUO78148.1"
                     /translation="MLKSLFSSSIRADVLSLLLNSPDEKFYVREIAKLLRKNPSGIKR
                     ELDNLEKMGIVVSERVANLKYFQAEQDSPLFSELKNLITKSLGLPGAIKALIRASGAK
                     AAFIYGPYAEGEDVDAVALFVIGASESEDMVKKGLKDLQKKFGQKISYTLMDEDEYRT
                     KRKKKDGALRRLLSGKRISLIGRV"
     gene            3569..4096
                     /locus_tag="VMU21_11300"
     CDS             3569..4096
                     /locus_tag="VMU21_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78149.1"
                     /translation="MIRKHALIAGIWLLLVFVACSSQIDKNRFDRNRFDRVNSTVQAL
                     QHSISTSGDYPQLGALIQQLSSEIEALNVSVTSRRERDLLQEYSELLKMYQDGFLLWK
                     YRTEFSSHNFVPKGRIYVGQDVEPIVVKYHLPTETHIFEPTHQIWKSIAEDSIRIIWN
                     NTDAQVKIINTMLNY"
     gene            complement(4239..4751)
                     /locus_tag="VMU21_11305"
     CDS             complement(4239..4751)
                     /locus_tag="VMU21_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUO78150.1"
                     /translation="MEIDRKVKDIIVDIFDFPHVPYWFTVKQAIKIMKVTLPTREKTS
                     YPLGILVFDEKYNLLGMVSMKDILRAKEAELPKSAAVSHMPDGYATASSTSRVEREVT
                     KYAEKAVSEVMTPLKCFVEPDDPVTKAAYLMIDNDLMVMPVLERKKKLVGLVLLTEVF
                     DQLCSEIQKG"
     gene            complement(4793..5257)
                     /locus_tag="VMU21_11310"
     CDS             complement(4793..5257)
                     /locus_tag="VMU21_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO78151.1"
                     /translation="MTKKKDIKVLLVDDERAFVDTLAQRLKMRELNVSTVYDGEQALS
                     YVKGIEPDVMVLDLKMPGLHGIEVLREVKRRYPKMQVIILTGHGTDRDAEEAKRLGGF
                     DFMRKPADIDNLVRRIKEAYNEKIERMMTAVAFAEEGEFDTAQKIITKQDEE"
     gene            complement(5400..6095)
                     /locus_tag="VMU21_11315"
     CDS             complement(5400..6095)
                     /locus_tag="VMU21_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78152.1"
                     /translation="MDSEKERNLRISQMGFVGKMIGGFTHELKNHLAIIKETAGLIED
                     LLVFRKSEEKKDSAEYMSSLDSIALQVDKAVQLADYLNRFAHRMDHPFSTFSVHDAIE
                     ELIALRRRSARERGICLETDFHGAVPAIHSDPSRLYCLLYCLIEEKMLGFDKGSHLII
                     KTEPSDSSIIIHIIPRGKPVESIAERIIDKGICMDEVVQEIIETLKAAVRHEQESVTT
                     ITVPLNLLPVEKK"
     gene            complement(6114..6488)
                     /locus_tag="VMU21_11320"
     CDS             complement(6114..6488)
                     /locus_tag="VMU21_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO78153.1"
                     /translation="MSWTKVLIVDDEVEFAAALSERLLLRDYDTKAVYNVDDAIAAIK
                     QDPPEVLLLDLKMPGMSGIELYRTIKEFNPSIEVIFVTGHACQDGDETIPREAFDCVM
                     KPIDIDELTKKIDKAKQSIIKK"
     gene            complement(6488..>7883)
                     /locus_tag="VMU21_11325"
     CDS             complement(6488..>7883)
                     /locus_tag="VMU21_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78154.1"
                     /translation="RKEFGEIVDLGLINSEGIQVSYAGPYHLKGREYSNQDWFQEVII
                     RGVYISDVFTGYRKMPHFAIAVRKEIPGKHAFWVLRATVDVEALNRFLSSIQLKEYDD
                     AFIINKDGILQTSSRFHGKMLERAALKFPVPAQDLSLIDYKQEGGGRLEVGFARIKQS
                     PWVLGMVVQSTASSRMIELFTKDVGTLYLIIILVIIGSIANSIITSSVVNRVQEAEQR
                     REAAIAETEHTSKLASIGRLAAGIAHEINNPLAVINEKAGLMKDILQLIGDFPNKEKF
                     LSIVFSITDSVDRCRTITQRLLGFARRMDVSVEIIDMNETIKEVIGFLDKEILYRNVR
                     LDMNLSENLPKIESDRGLLQQVFLNIANNAIDALKDGGTLAIATGKKDEDTLQVIIGD
                     NGHGIPKEVLKHIFEPFFTTKEKGKGTGLGLSISYGIMQKLGGTILVESEVDKGSTFT
                     VEVPVKASIDLRGL"
BASE COUNT         1982 a         1936 c         1774 g         2191 t
ORIGIN      
        1 cagttatgat gctccgggcg gggcgggctg tggggggaag aatgcctgtg gtcatctctt
       61 cagaggcagg ggggacaatg atccatgaag cgataggcca tggccttgaa gcagaccttg
      121 cacagcatgg attgtccgtc tactccaata aattaggtca ggaagttgcc tcaagactgg
      181 taacagtcat agacgatgca actctgcccc acaaaagagg ctcttacctg tttgatgatg
      241 aaggtacccc ctccaggcga acagtccttg tggataaagg gatactttcg ggataccttt
      301 atgatcaatt gtccgccatg catgacgggg tgaaatccac cggtaatggt cggagggagt
      361 cttaccagca caggccaata ccaaggatga gtaatacgtt catcgcgcct ggtgaagcat
      421 cgcctgaagc ggtggtgaaa tccacatgga atggcctttt cgtgaaaaaa atggggggag
      481 ggcaggtaaa taccgtcact ggagacttcg tgttcgatgt tcaggaaggc tatacaattg
      541 agagcgggac cattggtgaa cctgtacggg aagccactct tacaggcaac ggcccctcta
      601 tactgcgatc catagatatg atcgcttctg atctgggttt ctccataggg acctgcggaa
      661 aggactccca gggggttccc gtctctgacg caatgcccac cttgagaata agtgagatgg
      721 tggtcggtgg cgagatttct cgctcaaggt gacccgagta atagatgaat ccctaaccca
      781 ttaagtgttg caataattac acacctgaag aatattatca acacccttat gaatcaaaag
      841 tcttttattt aaaaggtttt tttctggcat tttccttgca cactaaattt ataactttat
      901 gcgtttacag aggactttaa gacaggaagt aacctttagc gggatcggcc tgcatacggg
      961 aagacatgcc gttgtgagac tgaagcccgc tccccgggac tccggtatca tcttcctcag
     1021 gatagataga atggcagtaa tacgggcgca tatcggtgcg gtaaccgaca ctgcatttgc
     1081 caccaccctt ggtaatgaca atataaaaat cagaacagtt gagcatctcc tcgctgccct
     1141 ggcaggtata ggaattgata atcttatcat tgaggtagat ggtcctgaga tacccatact
     1201 ggatgggagt tccaccgagc tggtaagtat aatattgcaa ggtggaatcg caaagcaggg
     1261 aaagaaaatg ccctgcttgc ggattacaaa acccataatt atggaggatg gtcattctga
     1321 gattgccgtt gtcccttatg agggaagaag aattaccttc aggattcatt tcccccatca
     1381 tttccttggt gaacaggagc tcagcataga actgaacgag gagacattca tcaaagaggt
     1441 tgcccctgca agaacctttg gttttctgaa ggacattcag tatatgatgg ccaatggcct
     1501 cgcgaaaggc ggctcactaa ataatgccgt catactcagt gacaagggtg tattgaactc
     1561 ttccgggctc aggttcaagg atgagtttgt gagacataaa atcctggact ctatcggaga
     1621 cttttccctg ataggctttc cgatatacgg acacatattt gcacacaagg caggacactc
     1681 cacgaacctt aaatttatta agaaagtcat ctcttgttct gattgctgga agattgtgtc
     1741 agaagctgaa caaccccagg cccaagctca gctccatctc taaaaaagat agtaatatca
     1801 agaagttaca aatttgtctt gaattttttc tgaaaatggg gtttcatatc tgaggaagac
     1861 cgcaaataaa aaaaagaggc tggagaatcc cttctccagc cctaagtccc acaagacttt
     1921 tcttatttct tctttgcggt cttcttcgct gtcttcttcg ctgcgggttt cttcgctgct
     1981 gccttctttg tcgccattat ttttcacccc ctttctatcc ccggaaaatc ggggaagctt
     2041 aaactaaacc ctcccgataa gcgaaatccg ctttccggaa aggagcctgc gcaacgcgcc
     2101 atcctttttc ttcctttttg tcctgtactc atcctcgtcc atcagcgtat agctgatctt
     2161 ctgcccaaat ttcttctgca gatccttgag cccttttttt accatatctt ctgattccga
     2221 agcgccgatc acaaaaaggg caacggcatc cacatcctca ccttctgcgt acggtccata
     2281 aatgaaggcc gcctttgccc cggatgccct gatcaatgcc ttgatcgctc ccggcagtcc
     2341 aagggacttg gtgataaggt tcttgagttc cgagaaaagg ggcgagtctt gttccgcctg
     2401 aaagtatttg aggttggcaa ccctctcgct cactacgatt cccatcttct caaggttgtc
     2461 aagttctcgt ttgataccgg aaggattttt tcggagcagt ttcgcgattt cacggacata
     2521 gaacttctca tctggactgt tcaggagcag tgagaggacg tctgctcgta tggatgatga
     2581 gaacaagctc tttaacatct tgctatagtt atatactaac aaattcaatt tgtcaacaaa
     2641 aaaattacaa ttaagaacag aaaatgcact tttgtgaacg atgcataaaa gtgaacgatt
     2701 gtttttttta tgaaactatc gttagacatt ccgttacttc ttctttctca aacgatagat
     2761 ttattttcat gaacggataa tgcttttttg tgaacgatcg tttttttcgc gtatttctct
     2821 tgtgagaaga aagtgctcac atggagcgtc catgaggtac tccctcatga gggcgcgccc
     2881 ttggcaggcc taaaagatta tctcttcggg aggtagtgaa gcgcgaatag ctcaggagcc
     2941 gattccctgg cgatcctagc gggattcgaa cccgtgttgt cggactatcc ttcccacata
     3001 gaataagtgc ttgcctaaat caattcggat ccaccttagc cgaaatttct cataagggaa
     3061 ctgccccggg gacggagcgg acgcctcttt ggctggagcg agccagggcc cttggcttgt
     3121 taaatatgcc acatgttaac ggtcagggtt atttagagtg tatcagcaag gcgtaacagt
     3181 ctaaatctac aaacgtgatc tggttggtga agttctcgca attgggtgtc ccggcttaca
     3241 tgaggaagta tgatcgctcg ggcaagcgag gagttgtcaa ggaatactcc ggtacaaggt
     3301 ttccgttggc attagatagt cagagaagat ccggcagata gggtttcgtg cagtaatcga
     3361 gcttcttata atgaggtatg cacccgagga aagcctttcg tttcaggatg gtttggtgta
     3421 actattcgcc cggacagaaa ataaggtaca atgaaatata ctgactgaat gaactataca
     3481 tcagaaatag agtctgtgcc gagaaaagga gtgaaaggca catgaaaata atattagcca
     3541 atcccgggct gaagatttcc aaacgcatat gataaggaaa catgctttaa ttgccggcat
     3601 atggttactc ctcgtctttg ttgcctgttc ctcccaaatt gataagaaca gatttgatag
     3661 aaacagattt gacagggtga actctactgt ccaggccctc caacactcca tctcgaccag
     3721 tggcgactac ccgcaacttg gtgcattgat ccagcagctg tcctcagaga tagaagcgct
     3781 gaacgtgagc gtgacatcca ggagagagag ggatctttta caagagtatt cagaactgct
     3841 caagatgtat caggacgggt ttctcttgtg gaaatatcgc acggaattca gcagtcataa
     3901 tttcgttccg aaggggcgta tctatgtggg gcaggatgtg gaacctattg tggtgaaata
     3961 ccaccttccc accgaaacgc atatatttga gcctactcac cagatatgga aaagcatagc
     4021 agaggactca atccggataa tttggaataa tacagatgca caggtgaaaa taataaatac
     4081 catgttgaac tattgatccc cttcaagaac gtctccgtct atcttttcaa tatcctccac
     4141 agggtagtct cggggatacc aaaatctttc ccccttgtca aaagagcggc caatcttgcc
     4201 cggggcttca gattctctga cctggaatta tcagaccatc agcccttctg aatctcgcta
     4261 caaagctggt caaagacttc tgtgagaagc acaaggccta cgagcttctt ttttcgctct
     4321 aagacgggca tcaccatcag atcattatcg atcataagat atgcagcctt ggtaactgga
     4381 tcatccggct cgacgaaaca tttaaggggc gtcatcactt cacttaccgc tttttcggcg
     4441 tactttgtca cttctctttc aacccgggag gtagacgacg ctgttgcgta tccgtcaggc
     4501 atgtgagaga ctgcggcgga ctttggtaat tcggcctctt ttgccctcag gatatccttc
     4561 atgcttacca tgcccagcag gttatatttc tcatcgaaaa caaggatgcc gagaggataa
     4621 gaagtttttt ctctggtggg aagtgtgacc ttcataatct ttatggcctg ctttaccgtg
     4681 aaccagtaag gcacatgagg gaaatcgaag atatcgacga ttatgtcctt tacctttctg
     4741 tcaatttcca tgggtaacac cgacgcacat cgaggaggtt ctacagccgc tgctattctt
     4801 cgtcttgttt cgttataatc ttttgtgctg tgtcgaattc gccttcctcg gcaaaggcta
     4861 ccgcggtcat catcctctcg atcttttcgt tataagcctc tttgattctc cgtacaaggt
     4921 tgtctatgtc cgccggtttc ctcataaaat caaagccgcc gagccttttt gcctcctcgg
     4981 catccctgtc cgtgccatgc cctgtgagga tgatgacctg catcttggga taacgccttt
     5041 tcacctctct cagaacctct atcccgtgta gtcccggcat ttttaagtcc agcaccatta
     5101 cgtcgggctc tattcctttg acataggaga gagcctgttc cccgtcgtat acggtgctca
     5161 cgttcagctc cctcatcttc agtctctgtg cgagagtatc cacaaaagcc ctttcatcat
     5221 cgaccaatag cacctttatg tccttctttt ttgtcatctt tcctcctccg tttcacactt
     5281 cagtcgaatc ttctcgcgcc gctacctaac tataacagcg gccactggcg gtgtcaagat
     5341 attgtataag ttgtgctgag ccttacaccg aagaagttcc tgctacccca tcatgcagct
     5401 tacttttttt caacaggcag caaattgaga ggaacagtaa tcgtcgtcac gctctcctgt
     5461 tcatgtctta ccgctgcctt caatgtttcg ataatctcct gaaccacttc gtccatgcag
     5521 atgcccttgt caataatcct ttcagcaatg ctttcaactg gcttccccct cgggatgatg
     5581 tggataatta tagaactgtc cgaaggctct gtttttatga tgagatggct gcccttatcg
     5641 aatcccagca ttttttcttc gatgaggcaa taaaggaggc agtagagtct tgaagggtcg
     5701 ctgtggatcg ctgggacggc accatgaaaa tccgtctcga ggcagattcc tctctcacgg
     5761 gccgatctcc gtctcaacgc gatgagttcc tcgatcgcat cgtgaacgct gaaagtcgaa
     5821 aaagggtggt ccatcctgtg ggcgaagcga ttcagataat cggcaagctg taccgcttta
     5881 tccacctgca aagcaattga atcgagagag ctcatgtatt cggctgaatc ctttttctcc
     5941 tcggattttc tgaacacaag gagatcctct atcaaaccgg cggtctcctt gattatggcg
     6001 agatgattct tcagctcatg ggtgaaccct cctatcattt ttccgacaaa gcccatctgg
     6061 gaaatcctca aattgcgctc tttttcggaa tccatcgtcc ccccccgaaa cggttacttt
     6121 ttgattatgc tctgctttgc cttatcgatt ttttttgtca gttcgtcgat gtctataggc
     6181 ttcatgacac agtcaaatgc ctctcgtgga attgtttcgt ctccatcctg gcaagcatga
     6241 ccggttacaa agatgacctc tatcgaagga ttaaactcct tgatcgttct gtacagttct
     6301 ataccactca tgcccggcat tttaagatcg agaagaagca cctcaggcgg gtcctgtttg
     6361 attgcggcga tggcgtcatc cacgttgtac accgccttcg tgtcgtaatc ccgcaggagc
     6421 agcctttccg acaatgccgc cgcaaattca acctcatcgt caacgataag cactttagtc
     6481 catgacatta taaccccctt agatcgatgc tggctttgac aggcacttcg actgtgaaag
     6541 tcgatccctt gtcgacctcg ctttcaacaa ggattgtacc ccccagcttc tgcattatcc
     6601 cgtatgatat ggagagaccg agacccgtac ctttcccttt ttctttcgtc gtaaagaagg
     6661 gctcaaaaat atgtttcaag acttctttcg gaataccatg accgttatcc cctataatca
     6721 cctgaagtgt gtcctcatct ttcttccccg tggcaatcgc cagcgtccct ccatccttca
     6781 gggcatctat ggcgttgttc gcaatgttta agaatacctg ttgaagcaat cctctgtcac
     6841 tctcgatctt gggaagattt tccgagagat tcatgtcgag gcggacattc cgataaagaa
     6901 tctctttatc gagaaatccg atgacctctt taatcgtctc gttcatgtca attatctcaa
     6961 cggaaacatc cattctccgg gcaaaaccca ggagtcgctg cgtaatagtc ctgcagcgat
     7021 ccacgctatc agttatcgaa aagacgatcg acaggaactt ttccttattg gggaagtcac
     7081 cgataagctg caggatatcc ttcatgagtc ccgccttttc attaatcacg gcaagtgggt
     7141 tatttatttc atgtgcgatc cctgccgcaa gccttccgat tgaggcaagt ttgcttgtgt
     7201 gttcagtctc cgcaatagct gcttcacgcc tttgctccgc ctcctgcacc cggtttacca
     7261 ctgaagaagt gatgatgctg tttgctatgg aacctataat aaccaatatt attatcaaat
     7321 aaagcgtgcc cacatccttt gtaaaaagct cgatcatcct cgacgaggcg gttgattgaa
     7381 cgaccatccc caatacccag ggggattgtt ttatacgtgc aaatccgacc tcaagacggc
     7441 caccgccctc ttgcttatag tctattaatg acagatcctg agccgggacc ggaaatttca
     7501 gagctgcccg ttcaagcatt ttgccatgaa agcgggaaga ggtctggagg atcccgtctt
     7561 tgtttataat aaaagcgtca tcgtactcct ttagttgaat tgaagaaagg aatctattga
     7621 gggcttccac atctacagtc gcacgtagta cccagaaggc gtgcttgccg ggtatttcct
     7681 ttctcacggc tatcgcaaaa tggggcattt ttcggtatcc ggtgaaaacg tcgctgatat
     7741 agacgccacg gatgattacc tcctgaaacc agtcctggtt cgaatattcc ctgcccttga
     7801 gatgatacgg tcctgcatag gacacctgga tcccctccga gttaattaag ccgaggtcaa
     7861 caatctctcc gaattctttc ctg
//
LOCUS       DATKOU010000238         4960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1507493, whole genome shotgun sequence.
ACCESSION   DATKOU010000238 DATKOU010000000
VERSION     DATKOU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507493"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="VMU21_11330"
     CDS             complement(<1..2390)
                     /locus_tag="VMU21_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HUO78155.1"
                     /translation="MSIKNLEVFFNPKRIAVVGASEDPESPGYFILRNMIGKGFRGVV
                     YPVNPKLESVQGVEAHKTVSAIPHPVDLAVLAVPFEEILPTFEECGRMGVKGVSIICP
                     DFARKVEDDPLFTPRATELLSRYEFRILGPNTIGFIRPSIHLNASLFPKMPKSGSTAF
                     ISQSATLTTALLDRAIDKNFGFSYIISVGEKLDLAFSDLIDFLGVDPKTRAIILYLEN
                     IVRGRKFMTAVRSFARSKPIVVVKSGKFDISAQVALTHSGVLAGEDKIYDAVFKRAGA
                     VRIDEILDLFYLAETLAQEKRPKGKRLAIVTNAGAPSMIAVDTLLKMEGELAVFEEKT
                     VASLRQRLPLLRQPWNPVNLASDASPDDYKTAVENCLGDNNVDGLLVMHVPHFRIRPQ
                     ETAEAIVSAKQINPRIPLFTVWMGGEMVHNAREFFTTKTVPTFVTPEQAVRSFIYLYR
                     YDYNLQLLQETPESILRDFSPDRERVKGIIREARVQKRLILSIHEVKEILLAYGIPVT
                     TTMRVESEEEAVRIADEIGYPVVLKIDSEKIFHKTEKSGVLLDLKSEDSVREAFRKLR
                     ELAAASQDPQAHVLIQQMMTAHGYELAIGAKKDPAFGSAIVFGLGGRLLEALGDYAVG
                     LPPLNQTLARRVMEETKIYKFLLAGDHYRETLRFMEEMLVRFSYLLIDFPEFKEIDID
                     PFFITEQGGAALDAGILLEEELTQHPKGFRSDLCPPHLSICPYPFKYVQEITLENRVP
                     ALIRPIRSEDEPLIYHLFKTVSEETIVFRFNQRLTDMPHERLARYCQLDYERELAFV"
     gene            2633..2709
                     /locus_tag="VMU21_11335"
     tRNA            2633..2709
                     /locus_tag="VMU21_11335"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2667..2669,aa:Pro,seq:cgg)
     gene            2724..3176
                     /locus_tag="VMU21_11340"
     CDS             2724..3176
                     /locus_tag="VMU21_11340"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HUO78156.1"
                     /translation="MKKIIGVIGGRRVDRTLLNEAEKVGTLVAQKEIVLVCGGLSGVM
                     EAVAKGARSAGGTTVGILPQEHKRDANEYIDIPIVTGLGIGRNVIIARTADVLISIGG
                     EYGTLSEIAFALQMGKPVIGIRTWDIQGVKKVENAEEAVAKAFELLES"
     gene            3239..3312
                     /locus_tag="VMU21_11345"
     tRNA            3239..3312
                     /locus_tag="VMU21_11345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3273..3275,aa:Arg,seq:cct)
     gene            3532..3885
                     /locus_tag="VMU21_11350"
     CDS             3532..3885
                     /locus_tag="VMU21_11350"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO78157.1"
                     /translation="MIEAAQEKRRAERYKGRFKIEYALGDQMYRGISSDLSLNGIFIR
                     TRNPLEMGTLITVVLHLPGGSTATLRGKVARVLVTPEGISAISIRNGMGIEIIEKDYN
                     YLRTVMSLVGGMKSN"
     gene            complement(4089..4949)
                     /locus_tag="VMU21_11355"
     CDS             complement(4089..4949)
                     /locus_tag="VMU21_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78158.1"
                     /translation="MKKSARSSILVTLFLILGISVFLGTIGLGLAQTSPSTALSNDDC
                     IKCHAVPPADMMANGGKHKAVGCVGCHTGHRPAVKDNIPKCNMCHMGKPHYELKDCLG
                     CHKNPHTPLMISFASNVTDACVTCHTQQITQLRDQKSKHTALNCSTCHNVHGKIPACM
                     QCHKPHSADMTVADCKKCHKAHMPKAVSYAADIPSKDCGSCHTNAFALLTASSSKHKA
                     LACAFCHQAVHKMVPKCQDCHGVPHAAGMMARFTKCGDCHNTAHDLNHWTPGAKKEEP
                     KEPKEIKKKK"
BASE COUNT         1170 a         1194 c         1325 g         1271 t
ORIGIN      
        1 acaaaggcca gctcacgctc atagtcaagc tggcagtagc gggcgagcct ttcgtggggc
       61 atgtcagtga ggcgctggtt gaatctgaat acgatagtct cctctgagac ggtcttgaag
      121 aggtggtaga tgaggggttc atcttcagaa cgtatcggtc ttatcagtgc gggtacgcga
      181 ttttccaggg taatctcctg cacgtatttg aagggatagg ggcagatgga gagatgcggc
      241 gggcagaggt cgctcctaaa cccttttggg tgttgtgtaa gttcctcttc cagcagaata
      301 cccgcgtcca gggcagctcc cccttgttcg gtgataaaga aggggtcgat atcgatttcc
      361 ttgaactcgg ggaagtcgat gagcagatag gagaatctca ccagcatctc ttccatgaat
      421 ctcagggttt ctctgtagtg atctccggcc aggaggaatt tgtaaatctt tgtctcctcc
      481 atgacacgtc ttgcgagggt ctggttgaga gggggcagtc ccaccgcata atctcccaag
      541 gcttcaagaa gcctcccccc caggccgaag acaatagcag agccgaatgc ggggtccttc
      601 tttgccccaa tggccagttc ataaccgtgc gcggtcatca tctgctggat caggacatgg
      661 gcttggggat cttggctggc tgccgcgagt tccctgagct tcctgaatgc ctccctcacc
      721 gaatcctcgc tcttgagatc gaggagcacc ccggacttct ctgtcttgtg aaaaatcttt
      781 tccgagtcta tcttgagtac tacggggtag ccaatttcat cggcaatgcg aaccgcttcc
      841 tcttcactct ccaccctcat ggtcgtagtc acggggatcc cgtatgcgag gagtatctct
      901 ttgacttcgt gtatactgag gatcagcctc ttttgaaccc gggcctcacg aatgatgcct
      961 ttcacccttt ccctgtccgg ggagaaatct ctcagaatgc tttcgggtgt ttcctgcaaa
     1021 agctgcagat tatagtcata gcggtacaga tagatgaaac tccttaccgc ctgttcgggg
     1081 gtgacaaaag tggggaccgt ttttgtggtg aaaaactccc gggcattatg taccatttcc
     1141 cctcccatcc atacggtgaa caagggaatg cgggggttga tctgctttgc agagacgatg
     1201 gcctctgccg tttcttgggg acggattcta aaatggggaa catgcattac caggagtccg
     1261 tccacattat tatcgccgag acagttttcg actgcggtct tgtagtcgtc aggagaagca
     1321 tcactggcga gattgacagg attccatggt tgtctcagta aagggagcct ctggcgcaag
     1381 ctggctacgg tcttctcttc gaataccgcg agttccccct ccattttcaa aagggtgtcc
     1441 accgcgatca tggagggagc gcctgcgttg gtgacaatgg cgaggcgttt ccctttgggc
     1501 ctcttttcct gggcaagggt ctccgcaaga taaaagagat caaggatctc gtcgattctc
     1561 actgcacccg cccgtttgaa tactgcatca tagattttat cttccccggc aagaacccct
     1621 gagtgggtaa gggccacctg ggcagaaata tcgaacttcc cggatttgac caccacgatg
     1681 ggcttactgc gggcgaaact cctcaccgcg gtcatgaact tacgtcctct cacgatattt
     1741 tcgagataaa ggatgatggc cctggttttg gggtctactc ccagaaagtc gatgaggtca
     1801 gaaaacgcaa ggtccagttt ttcgccgact gagatgatat agctgaagcc gaagttcttg
     1861 tctatggccc tgtccagcag cgcggtggtg agagtggcgc tctgggaaat gaaggctgtg
     1921 gagccgctct tcggcatttt ggggaaaagg cttgcgttca aatgtatgct cggcctgatg
     1981 aacccgatgg tattgggacc cagaatcctg aactcgtatc tcgacaggag ctctgttgcc
     2041 cggggagtaa agagtggatc gtcctccacc ttgcgtgcga aatcgggaca tatgatggac
     2101 acgcctttca cccccattct gccgcactcc tcaaaggtcg gaagaatctc ttcgaagggc
     2161 acggccagga cggcgaggtc cacgggatgg ggaatggcag agactgtttt gtgtgcctca
     2221 accccctgaa cagactccag cttgggattg acgggataca ccacacccct gaagcccttg
     2281 ccgatcatgt ttctcagaat gaaatatccg ggggattccg gatcctcgct tgcgcccaca
     2341 accgctattc tttttggatt gaaaaaaact tcaagattct tgattgacat agcggatctc
     2401 ctttaccgaa attccctcaa ttcacgggtg cgcctgcgga ctccgagcag gccccacgtt
     2461 tcttcttcta ttctacacga ggaaatgcgg catggatagt tccggtcttc atatgcaggt
     2521 ggtgcaaaag aataccatca ggaataaata gcaatttaca aaccgcgtta ttttgaagta
     2581 aaattcaggg gtgtggggtg ggacgcattc ccgcagtctg ccgccatact atcggggcgt
     2641 agcgcagtct ggtagcgcac acggttcggg accgtggagt cgaaggttca aatcctttcg
     2701 ccccgaccat ttttatccag gccatgaaaa aaatcatcgg ggttatcgga ggaaggagag
     2761 tcgataggac gctcctcaat gaagcggaaa aggtggggac ccttgtcgcc caaaaagaaa
     2821 tcgtccttgt ctgcggcggg ctcagcggtg tcatggaagc agtagcaaag ggggcccgat
     2881 cagcgggcgg cactacggtc ggaatattgc cccaggagca taaacgggat gccaacgaat
     2941 acatcgatat cccaatcgtt actggactgg gaatcggaag aaatgttatt atagcgcgca
     3001 ctgcagacgt cctcatctcc attggcggtg aatacgggac gctctctgaa atcgccttcg
     3061 cgctccagat gggaaaaccg gtaatcggca tcaggacatg ggatatccag ggcgtgaaaa
     3121 aggtagagaa tgctgaagaa gctgttgcaa aggcttttga gcttttagaa tcgtgaattt
     3181 ttcttttctg tttttagaac tttaaacttt tcattttcaa ctttgaactc ttttagtcgc
     3241 ccccttagct caggtggata gagcacaggt ttcctaaacc tggtgccgca agttcgaatc
     3301 ttgcaggggg cactttattt ttcaaggagt taccgccatt ccggggggcg tttttcccat
     3361 aagtatttgt cctctccctc acccctttca agaagacact atacgttaca atcctacttt
     3421 gggaggaatg gtccttcccg aataagaaaa ataatacccc tacctgggta ggattgattt
     3481 tattttcact ccagcccaaa atgataaata tatgataccg cagggattaa gatgatagag
     3541 gcagcccagg aaaagagacg tgcagagagg tataaaggac gttttaagat tgaatatgcc
     3601 ttaggcgatc aaatgtatag gggtatatcc agcgaccttt ccctgaacgg gatcttcata
     3661 aggacaagga atcctcttga gatggggaca ctaattaccg tggtacttca tctccctgga
     3721 ggctcgaccg caacattaag gggaaaagtg gcgagagtat tggtaacgcc tgaggggata
     3781 tctgccatat ccataagaaa cggaatggga atcgaaataa tagaaaagga ttataattat
     3841 ctcaggactg tcatgtcatt agttggcggg atgaagtcca attgagcttc tcaccttcat
     3901 ggttcaaatg agcaggtaag aactgtattg attgagtccg gtcataaaga gcagctaagc
     3961 gccccatgag ttccgtccga taaataaact cagccttgat ggcctgacac tttagtgtca
     4021 tagttataca ttgacaaaaa aagggcctgt aatcctacag gcccttatat caatgcacag
     4081 agaatcggct atttcttttt cttgatttct ttgggttcct taggttcttc cttttttgcc
     4141 cctggtgtcc agtggttcaa atcatgagca gtattatggc aatcaccgca tttcgtgaat
     4201 ctggccatca tgcctgcggc gtggggaaca ccatggcagt cctggcactt ggggaccatc
     4261 ttatgcacag cctggtggca gaacgcacag gcgagtgcct tatgcttgga gctgctggcg
     4321 gtgagaagtg cgaaggcatt cgtgtggcac gagccgcagt ctttggaagg aatgtctgct
     4381 gcataagaca cggccttagg catgtgtgcc ttgtggcatt tcttgcaatc cgcaactgtc
     4441 atatcggctg agtggggttt gtggcactgc atgcaggccg gtatctttcc gtgaacgtta
     4501 tgacaggtgg aacagttgag cgcggtgtgt ttgctctttt gatccctgag ctgggtgatc
     4561 tgctgggtat ggcaggtcac gcatgcgtcg gtgacattgc tcgcaaaaga aatcatgaga
     4621 ggggtatgag gattcttatg acagcccagg cagtctttga gttcataatg gggtttgccc
     4681 atgtggcaca tgttgcactt gggaatgtta tcctttaccg caggcctgtg gccggtatgg
     4741 cacccaacac atcctacggc tttgtgtttc cctccattgg ccatcatgtc agcaggaggc
     4801 actgcatgac acttgataca gtcatcgttg gacagggccg tgctgggaga tgtttgggcc
     4861 aatcccaggc ctattgtgcc cagaaaaacc gagattccga gaatcaaaaa taatgtgaca
     4921 agaatactac tgcgtgcact ctttttcatc tctcctcctt
//
LOCUS       DATKOU010000239         4427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5348404, whole genome shotgun sequence.
ACCESSION   DATKOU010000239 DATKOU010000000
VERSION     DATKOU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348404"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2100
                     /locus_tag="VMU21_11360"
     CDS             205..2100
                     /locus_tag="VMU21_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUO78159.1"
                     /translation="MENLITKNDVLKGLNPQQRAAVEYLDSPLFLSAGAGSGKTRVLT
                     HKIAYLVKEKGIKPHRILAITFTKKAANEMAERVERMLGIKPRWISTFHSFCVKILRE
                     DIKDIGLAFDQKFVIYDEDDSIKVLKDVLKRQNRDPKEAPSAREVISRAKQAYRKSIV
                     DHITALPYPASSYAETARAYQEDLARSNAMDYDDLLYFTVHLLSVSPEVRKRWQEKFD
                     YLMIDEFQDTNDIQFDLIKLLGDGKKGLFAVGDFFQCIYTWRGSRPSNIGRFIKEFKA
                     KEMRLEKNYRSTRKILDIANTVVSKVNCGWSETVLELHTDREEEGEVKYVNKPDGMSE
                     SIWIAGKIGELSSYHSYSDIAILIRMTFLSRVLESVFMQYNIPYTIVRGLAFYERAEV
                     KDLLSYLRLISNHRDKAAFERIINSPSRGIGKKGFSLIRGSFKTDWIQALMDTKMPMR
                     QRMNADAFIATIQKHSSSVEERPYVVLMDLVEDLRYFDYLRKEYKDDYEDRVQNVSEL
                     ANVLEKLQEEGKPFSEFMEDSLLSSDQDRIGNEDSVKILTLHAAKGLEWPVVFIPALE
                     EEIFPSAKSIFSESALEEERRLFYVGTTRAKERLYLSSADVRMKFGQTLHAMPSRYIE
                     EIRHHLK"
     gene            2279..2845
                     /gene="lexA"
                     /locus_tag="VMU21_11365"
     CDS             2279..2845
                     /gene="lexA"
                     /locus_tag="VMU21_11365"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HUO78160.1"
                     /translation="MRSRVKGISRFYYQKGRMPSFSEIGEIVGMSSKNAVYKLVNKLE
                     DLKVLEKDKKGRLIPRSIAAPVKMLGTVEAGFPSPAEEELVDTLSLDNLLIQNPEATF
                     LLKVSGDSMSEAGILPGDMVLVDKGETPKSGDIVIAEVDGEWTMKYLKKRGESVVLIP
                     ANPKYQPIRPKKELKIAGVVTAVLRKYR"
     gene            2869..4182
                     /locus_tag="VMU21_11370"
     CDS             2869..4182
                     /locus_tag="VMU21_11370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HUO78161.1"
                     /translation="MSNQPLTIRSWPRAILHLDADAFFASCEQAIHPELKGKPVITGK
                     ERGIVAAASYEAKARGVQRGMSLYEVKKVCPDVVIVPSDYETYSLFSVRMFGILRRFS
                     PDVEEYSIDEAFVDLTGLRRSFHGPYGMIAGNMQETVERELDITVSVGVSLSKVLAKI
                     GSKHNKPHGLTLIPGRDIHLYLEKLPIEKVWGIGPNTAAFLGKFGITTALQFARKDEG
                     FISKHLSKPYREIWDELNGRSVYPVTVESKSSYQSISKTKTFTPPSGDKTFVFAQLSK
                     NLENACIKARRYKLAATKLILFVRTQEFRDSGVELKLSRPSAYPSDLFGPLQKGFDHI
                     YQSNCSYRQTGVVLGDLLSDNNVQYTLFDDTARIEKMSRVYSTLDMLSEKFGKYAVHH
                     ATSLPTKLQTQHEGERGDVPQRTKDLFKGENKRQRLGVPVLHIKV"
BASE COUNT         1307 a         1017 c         1110 g          993 t
ORIGIN      
        1 taaaagaaaa catggataaa acgcccggat tctttcttac ggaaatcggg gatgatcacc
       61 tgaggaacat aaaataaggg atgcccctcc aacttggatt tgtcacgagc aatgtagttg
      121 cctattcttc aacggcggca agataagcgg ttttttttcg gataagaaga aagcctcaga
      181 tccgtataag gaaaacgaac agcaatggaa aaccttatta ccaagaatga cgtgctgaaa
      241 ggactgaacc cacagcagag ggctgcggtg gagtacctcg actcccctct attcctgtca
      301 gcaggggccg gcagcggaaa aaccagggtg ctcacccaca agatagccta tctcgtgaag
      361 gaaaaaggca taaagccgca ccgcatcctt gccataacat tcacgaagaa ggcggcaaac
      421 gaaatggcgg aacgcgtcga gaggatgctg ggcataaagc caagatggat cagcaccttc
      481 cactccttct gcgtaaagat tctcagggag gacatcaaag atattggttt ggccttcgac
      541 cagaaattcg tgatctatga tgaggacgac agcattaagg tcctcaagga tgtactgaaa
      601 aggcagaaca gggacccgaa agaggcgccg tctgcaagag aggtaatctc cagggcaaag
      661 caggcataca gaaaaagcat agttgaccac atcacggccc ttccgtaccc ggcatcgtct
      721 tatgctgaga ccgcgagggc ataccaggaa gacctggcac gctcaaatgc aatggactac
      781 gacgacctcc tgtatttcac cgtccatctg ctctctgtct cccccgaggt gagaaagaga
      841 tggcaggaga aatttgatta cctgatgatt gatgagttcc aggacactaa cgacatacag
      901 tttgatctta taaaactgct gggagacggc aaaaaagggc tctttgccgt aggggacttc
      961 tttcagtgca tctatacctg gagagggtcc cgcccctcaa acattggaag gttcatcaag
     1021 gagttcaagg caaaggagat gaggctggaa aaaaactatc gctccaccag aaagatcctc
     1081 gacatcgcaa acaccgtggt cagcaaggtg aactgcggct ggtctgagac cgtgcttgaa
     1141 ctccacactg acagggaaga agaaggggag gtaaagtacg tcaacaagcc ggacggaatg
     1201 tcggaaagca tctggatagc cgggaagatc ggggaactgt cttcctacca ttcttactca
     1261 gacatcgcaa tactcatacg catgaccttc ctgtccaggg tactggaaag tgtcttcatg
     1321 cagtacaaca tcccctacac cattgtgaga ggactggcgt tctatgagcg ggcagaggtt
     1381 aaggacctgt tgtcgtatct caggttaatc tcaaaccaca gggacaaggc tgcctttgag
     1441 cgtatcataa attccccttc ccgggggata ggcaagaagg gattttcctt aataagaggg
     1501 agcttcaaaa ccgactggat ccaggcgtta atggacacca aaatgccgat gagacagagg
     1561 atgaatgcgg acgcattcat cgcaaccatt cagaaacatt cctcatctgt ggaagaaaga
     1621 ccctatgtgg ttctcatgga ccttgtcgaa gacctcagat attttgacta tctgcggaaa
     1681 gagtacaagg atgactatga agacagggtg caaaacgtat cagagcttgc caacgtgctt
     1741 gaaaagctgc aggaagaggg taaacccttc tctgagttca tggaggattc ccttctttcc
     1801 tcagaccagg acaggatagg gaacgaagac tcagtcaaga tcctcactct ccatgcggcc
     1861 aagggacttg aatggccggt ggtgttcatc cctgcccttg aagaggagat attcccctcg
     1921 gcaaaatcta tattcagcga atcggccctt gaagaggaaa ggagactttt ctatgtggga
     1981 acaacccggg caaaagaaag gctctacctc tcatcagcgg atgtacggat gaaattcggg
     2041 cagaccttgc acgcgatgcc ttcacgttac atagaagaga tacggcacca cctcaaataa
     2101 cagatgggct ggctcgaatc agctacattt ttcaaagagc tgcttcttac aaaacggccg
     2161 cacataccct aacttatcat aaccattaac tatttctata attcttctct tttcccttga
     2221 cagtaaacgt tcgttcactt ataatggggt catgaagaag ggctcacgag aagacaagat
     2281 gcgatccaga gttaagggca tatcccgttt ctattatcaa aagggaagaa tgccaagctt
     2341 ctcggaaata ggagagattg tgggaatgag ctcaaagaac gcagtttata aactcgtcaa
     2401 taaacttgag gacctcaaag ttcttgaaaa agacaaaaag gggaggctta tccctcggtc
     2461 aatcgccgcc cctgtaaaaa tgcttggtac ggtagaggca gggtttccga gtcctgcaga
     2521 ggaagaactt gtggataccc tttcccttga taatctcctc atacagaacc ctgaggctac
     2581 ctttctgctc aaggtgtcgg gcgattcgat gtccgaagcc ggcatactcc ctggagacat
     2641 ggtactcgtt gataaaggag agacacccaa gagcggagac attgtaattg ctgaagttga
     2701 tggtgaatgg accatgaaat acttaaagaa aaggggagaa agtgtggtgc ttataccagc
     2761 aaaccccaag taccagccta tcagaccgaa aaaggaactt aagattgcag gagtagtgac
     2821 agctgttcta aggaaatacc gatagtaaag ttttctcaag gagtcaccat gagtaatcaa
     2881 cctttgacca tacgttcctg gcctagagca atcctccatc ttgatgctga tgcctttttt
     2941 gcatcctgcg aacaggcaat ccatccggaa ttgaaaggaa agcctgtgat aacgggcaag
     3001 gaaagaggga tagttgccgc agctagttac gaggcgaagg caagaggggt acaaaggggt
     3061 atgagccttt acgaggtgaa gaaggtctgc ccggatgtag taatagtacc ctcggactat
     3121 gagacttaca gtctcttttc tgtcaggatg tttggaatcc taaggcgctt ctctccggat
     3181 gtagaggaat attccataga tgaggcattt gtggacctca cgggtcttag gagatctttt
     3241 cacggccctt acgggatgat cgctgggaac atgcaggaga cggtcgaaag ggaactcgat
     3301 attacggtat ccgttggggt aagcctttca aaggtccttg ccaagatcgg ctcaaaacat
     3361 aataaaccac atggccttac cctcataccg ggcagggata tccatcttta tctggagaag
     3421 ctgccgatag aaaaggtctg gggcattgga ccgaacacag cggcattcct cggtaaattt
     3481 ggaataacaa cagctcttca gtttgcaaga aaggacgagg ggttcataag caagcacctc
     3541 tccaaaccat accgggagat ctgggatgaa ctgaatggaa ggtctgtcta tccagtcacc
     3601 gttgaatcaa agagcagcta tcaatccatc agcaagacca aaacctttac cccaccttca
     3661 ggcgataaga cctttgtatt tgcccagctt tctaagaacc ttgagaatgc ctgcataaag
     3721 gcgcgtcgct acaaactggc tgctactaag cttattctct ttgtcagaac gcaagagttc
     3781 agagacagcg gtgtggaact gaaattgagc cgtccgtcag cctatccgtc tgaccttttt
     3841 gggcctttac agaaaggttt tgatcatatt tatcagtcca attgttctta tcgccagaca
     3901 ggggtagtcc ttggtgacct tctatcagac aacaacgtcc agtatactct cttcgacgat
     3961 acagcaagga tagagaagat gtcgagggta tacagtactc tcgatatgct atccgaaaag
     4021 tttggcaaat acgcggtcca tcatgctaca agcctaccga caaagctcca gacccagcac
     4081 gaaggagaaa ggggggatgt accgcaaaga actaaggatc tttttaaggg agagaacaaa
     4141 aggcagcgac taggcgtgcc agtgttgcat ataaaggttt gaatgaatta tgagagcttc
     4201 acctgagaca caacaggtat ttgcagctgc aacagacaag gacttcgtcc cgaaacttgc
     4261 ggcgcatgat aggagcgcga aagatgacca ttcgtcagtc tccactttcc gtatggaggc
     4321 ggccagtctt tatttctttt gataataaat ggacagcggc acgactgggc agacaatata
     4381 gtttcacccc ctactgtgca ataacaaggg ctggagacgg ttgacac
//
LOCUS       DATKOU010000240        16520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1885396, whole genome shotgun sequence.
ACCESSION   DATKOU010000240 DATKOU010000000
VERSION     DATKOU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885396"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VMU21_11375"
     CDS             <1..1081
                     /locus_tag="VMU21_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUO78162.1"
                     /translation="NIIVSRRAAIEEERQKKKVITLTKLKEGSRLEGVVKNITDYGVF
                     VDLGGIDGLLHISDISWGRITHPSEFFAVGDTVEVIVLKYDEELERVTLGYKQKKIDP
                     WLSVGEKYPLGAKVKGKVVSIADYGAFIELEEGVEGLVHVSELDWSARPKHPSKYLSI
                     GESVEAAVLRVDMEERRISLSIKQLKPSPWELVSQRYSVGQKISGKVRSITEFGAFVG
                     LPEGVDGLVHISDISWTKHIKHPSELLKKGQKVEAVVLSLEPEKERIALGMKQMQADP
                     WIKDIPEKYKLGDEVRCKILRLTDFGIFAEIEGEVEGLIYSSEVVKGEELLKEGEEVL
                     VRIIKIDTEDRKIGLSMKHVKGDQG"
     gene            1078..1926
                     /gene="sppA"
                     /locus_tag="VMU21_11380"
     CDS             1078..1926
                     /gene="sppA"
                     /locus_tag="VMU21_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HUO78163.1"
                     /translation="MKKVLLLLLGLLTVLVFLSLMIALLFRGSPMGDKVALVRIEGPI
                     VDSKNPIDEIKEYMKDPSVKAIVLRVDSPGGAVAPSQEIYEEVRKAVAKKKIVVSMGS
                     LAASGGYYISAPASRIVANPGTLTGSIGVIMEIPNLEGLMNKVGVKTEVIKSGRHKDI
                     ASVFRGIGREEREILQGVLDDVHDQFINAVAEGRKMLPDQVKKLADGRIFTGKQAVGI
                     GLVDELGNLEDAIKVAAKLSGIKGEPGVVSKKEKFSFMDIVRGNLPKELSDIFPTVKL
                     KYYFSP"
     gene            2004..2285
                     /locus_tag="VMU21_11385"
     CDS             2004..2285
                     /locus_tag="VMU21_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HUO78164.1"
                     /translation="MTKSALIDKVAEKVEGLTRKQTEIIVDTVFDGIRDALAKGEKIE
                     IRGFGNFRLKQRRPRRARNPKTGEGVDVPSKRVLHFKVGKALKDALNPQ"
     gene            2757..3590
                     /gene="nifU"
                     /locus_tag="VMU21_11390"
     CDS             2757..3590
                     /gene="nifU"
                     /locus_tag="VMU21_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HUO78165.1"
                     /translation="MWEYTEKVKDFFLHPKNVGEIEHPDAVGEVGSMLCGDALKLTLK
                     IDKKTGRIIDARFQTFGCASAIASSSVLTELIKGKTLDEALELTNQDIAEYLGGLPKE
                     KMHCSVMGREALEAAITNYRGETRPVEREEKVICKCFEVSEEKIRRVALENHLSTVEE
                     ITNYTKAGGGCGSCIPAIEAILKDIWSVKPECEAPKAHRKMTNLQRIALIQDVIEKEI
                     RPRLQTDGGDIELIDVDGKRVIVSLRAMCVACPMSDVTVKGIEDKLRELVSEDLVVEA
                     E"
     gene            3587..4759
                     /gene="nifS"
                     /locus_tag="VMU21_11395"
     CDS             3587..4759
                     /gene="nifS"
                     /locus_tag="VMU21_11395"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HUO78166.1"
                     /translation="MKPIYLDNNATTAVAPEVFEEMRPYLQEFYGNPSSMHTFGGQLY
                     RKIEEAREKVAALIGAVPEEIIFTSCGTESDNTALMSAIESYPAKRHVITTRVEHPAV
                     LNFCKHLARKGYRVTFLPVDSLGRLDMDAFSRAVDDDTAVVSVMYANNETGVVFPVRE
                     MGEVLRERKILFHTDAVQAVGKIPIDTRTLPVDMLSLSGHKVHAPKGVGVLYVRKGTR
                     FYPYIIGGHQERGRRAGTENVASLIALGKACELAGSAFRGEVDYMGRLRDKLEKALLQ
                     CCPDVRVNGDTEHRLPNTANISFEYVEGEAILLRMDEYGICASSGSACTSGSLEPSHV
                     LRTMGVSFTAIHGSIRFSLSRYTSDAEIDRVIEVMPGIIKELRGLSPYGRIKMVSG"
     gene            4947..6206
                     /gene="tig"
                     /locus_tag="VMU21_11400"
     CDS             4947..6206
                     /gene="tig"
                     /locus_tag="VMU21_11400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HUO78167.1"
                     /translation="MIKTVEDISATRKRLRIEIPADMIEKEIRDSLEKLRRKTTIPGF
                     RTGKAPINLIEKRFGKEVESDVLERIIPQGYVEALREANITPVASPVLEEKSDFKRRQ
                     PISMTLMVEVMPNIENLKYEDIPLKDIPVTVSDSDVESVLKRQQEEKATYEPSEGPVE
                     MNDLIVVDYSSPGDGVEAKDQIVKVGGSMFPEEFTRKLVGRGKGEELSIEATFPGDHP
                     LENLAGKHLTMKVMIKDIKKVNLPAVDDELAKDIGFQNLEEMRKRIGEEIEKAKRNEV
                     AKIQKAEIIRNLVESHEFDIPESLVERESEMLASAALSHRTEAAEPSEHELDALKQEA
                     RPNAMRNVKASLLIETIGKAQGVSVAEDEVKRAIISLAQRLSVSPEQIMKFYISRDGS
                     LEGLKGSLFEEKVLDLILSKAKLEKGE"
     gene            6208..6798
                     /gene="clpP"
                     /locus_tag="VMU21_11405"
     CDS             6208..6798
                     /gene="clpP"
                     /locus_tag="VMU21_11405"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HUO78168.1"
                     /translation="MSVIPIVIEQTGRSERAYDIYSRLLKDRIIFMGTAIDDAVANTV
                     IAQLLFLQTEDPDKDIHLYVNSPGGIVSSGLAIYDTMQYVKSDIATYCIGQATSMGAL
                     LLAAGTQKKRFALPHSRIMIHQPMGGFQGQATDIEIHAREILRTKDILNQILASHTGQ
                     SIEKIRTDTDRDFFMSGEEAKEYGIVDEVIHSMKKK"
     gene            6814..8061
                     /gene="clpX"
                     /locus_tag="VMU21_11410"
     CDS             6814..8061
                     /gene="clpX"
                     /locus_tag="VMU21_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HUO78169.1"
                     /translation="MAKKEKDETKLKCSFCGKGQDEVKKLIAGPTVYICDECVELCNE
                     IIAEELYAETTTHTLPKLPTPKEIHEFLNDFVIGQEKAKKILSVAVHNHYKRIYSPGA
                     SDVELQKSNILLIGPTGTGKTLLAQTLARLLDVPFTIADATTLTEAGYVGEDVENIIL
                     KLLQASDYEVERAQRGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKIIEGTMAS
                     IPPQGGRKHPQQEFIQVDTTNILFICGGAFVGLDGIVEQRTGKKTLGFGAEVIGAKER
                     KIGNVLSLVEPEDLIKYGMIPEFVGRLPVVALLDELDEGALVRILTEPKNSLIKQYQK
                     LLSLDNVRLRFTDTALAAISKRAIQRKTGARGLRAILEEVMLDVMYEIPSQKAIRECL
                     INEDTILKRERPILIYEKQAESA"
     gene            complement(8353..8814)
                     /locus_tag="VMU21_11415"
     CDS             complement(8353..8814)
                     /locus_tag="VMU21_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78170.1"
                     /translation="MKSLILTFLTLAIAAGMVTAAEMREIELTDGTILTGEIVSLTGG
                     VCTIRSATLGTVRVEESNIRTIRLKDSAGTSANTGGEIMSLQNKMMSDGEIMNMMQGL
                     QNDPEFQEILQDPEIMKAVQAGDVSALVANPKFMRLLNNLAVKDIKNKITR"
     gene            complement(8879..9325)
                     /locus_tag="VMU21_11420"
     CDS             complement(8879..9325)
                     /locus_tag="VMU21_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HUO78171.1"
                     /translation="MKRVGNLISPLVAGLGLEEAVRFERIKKEWSDIFREPLSLHTSP
                     CSLKHGELLITVDSPVWHQQLIFLKGEILKNVSPFGVKDVRFRVGTVRTKTKGAPPNP
                     PQKKIPLDRYSLQQIEETVSGLEDGPLKESIRKTMEKSLSARKVKA"
     gene            complement(9322..9510)
                     /gene="rpmB"
                     /locus_tag="VMU21_11425"
     CDS             complement(9322..9510)
                     /gene="rpmB"
                     /locus_tag="VMU21_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HUO78172.1"
                     /translation="MATCFVCGKGRVMGNNVSHANNRTKRQVLPNLQRTRIMINKGVH
                     REYVCTRCLRSGLVKKAL"
     gene            9759..12239
                     /gene="infB"
                     /locus_tag="VMU21_11430"
     CDS             9759..12239
                     /gene="infB"
                     /locus_tag="VMU21_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HUO78173.1"
                     /translation="MSKLRIYELAKKLNVTHKEVISELLRLGISVKSHASTVEDEIAG
                     KVEAIFRKKQKPAPVAKPKASKPKEAPVKKGKPSEIVPKPVKIQEAPVKAGRPPVVPP
                     KAEKPKIAEPKIEKPVAPRAREEKVEKSAVPIEKPEEKVEAEKKEEEKEYVSPAAEGK
                     TLEEPILPIEEEDLKVPDRFKKDIETEKVEKFKVKPAMQRAFQAVRKIEPKRWHEVKT
                     FKKPGRDRGVQKIAEKPQPQVTAPRKKMLKIQEGTTVKEFAELISVKLPDVIKKFMEL
                     GDMPTINQPVDMDAALLVAEGFGVKLEVAAGEEETVIEETAEDATSLSPRPPVVTIMG
                     HVDHGKTSLLDAIRETKVTETEAGGITQHIGAYKVTLKGKEIVFLDTPGHEAFTSLRA
                     RGAKVTDVVVLVVAADDGVMPQTIEAIDHAKAANVPIVVAINKIDKPEANPTKVKNEL
                     SARGIVSEEWGGQNIFVEVSAKKRIGIESLLEMILLQSDIMELKANAKKMAKGTIIEA
                     RLDRGRGPVATVLVQNGTLRIGDVFLTGISVGKVRALIDDTGNRISEAQPATPVEVIG
                     FSEVPTAGDLFTVVDDEKKARQIALARQQKQRLAEMARAKKLTLDEVYAKIKEGEIRE
                     LNIIIKGDVQGSVEALKEAFEQIIHPQVKVKVIHTSVGGINESDVMLAAASNAIIIGF
                     NVRPESKAHQVAEKEGVDIRHYNIIYEAIDDIRKALEGLLEPTLKEKILGRAEVRQTF
                     QVSRLGTIAGCYVVDGIISRTSDGIRIIRDNIVVYDGKLGSLKRFKEDVREVQTGYEC
                     GITIENYNDLKVGDVLENYVIEKIAAKL"
     gene            12417..12764
                     /gene="rbfA"
                     /locus_tag="VMU21_11435"
     CDS             12417..12764
                     /gene="rbfA"
                     /locus_tag="VMU21_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HUO78174.1"
                     /translation="MLPYKRSQRVSDLLRQEIADIIMRKVKDPRLGFVTVTGVDLTDD
                     LKIARVFVSCLKEEERETTLDILNAAKSFIRSEVGKRVRMKVLPSLEFRVDESVGYGD
                     RIEKLLREIKGNT"
     gene            12766..13713
                     /locus_tag="VMU21_11440"
     CDS             12766..13713
                     /locus_tag="VMU21_11440"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HUO78175.1"
                     /translation="MNIPDRLIQFILQERKFFIATHIRPEGDALGSSLALSLALESMG
                     KETTLYERDPVPALYRFLPGYKRFTHTHSPLAQDGLPLLLLDCNEPERAGLENVVIRR
                     SAVIDHHETIKEFGDIQWIEPHAAATGVMVYDLIRKLGIGITKDMATNLYAAIATDTG
                     TFRFSNTSSDVLRIAAGLVESGADPGVIAGELYETWSKGRFSLLTKVLNTLELTHGIA
                     VTVVTKAMFSETGTSPEDTENFSNFPKMIEEIKVSLFLREIDEGWKVSLRSKGDCNVA
                     EIAVRFHGGGHKNAAGCVIKGDLKTAKTQLIKAIEGIKG"
     gene            13793..14671
                     /gene="truB"
                     /locus_tag="VMU21_11445"
     CDS             13793..14671
                     /gene="truB"
                     /locus_tag="VMU21_11445"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HUO78176.1"
                     /translation="MNVVINLNKPKGISSQQAVTRVKRITGVRKAGHAGTLDPMATGI
                     LLVCTGEATKITRFLSDLDKEYLTVMKLGEKTDTYDSEGTVVQRSEGFSLGKEDIEKV
                     LAGFRGTIEQTPPMFSALKVGGRPLYALARRGITVERAGREITIHGIEITGFSLPFVG
                     MKITCSKGTYIRSLCNDIGEALGIGAHITDLQRTRIGGFRIENSVTIDDLEKSPAAAR
                     NAFVSVDEALGHLREISLDEGDFNRARNGLSVKLYASGLPQESFVRLKDPSGHLFALG
                     RVLENTVKIERILHLC"
     gene            14989..16038
                     /gene="pilM"
                     /locus_tag="VMU21_11450"
     CDS             14989..16038
                     /gene="pilM"
                     /locus_tag="VMU21_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HUO78177.1"
                     /translation="MFGKKGIIGIDIGSSYIKAVQLNETRSGFELELFDMLALPPELI
                     VESSIIDAIRVTESLKELVRQAKIKAKDAVISVSGHSSVIIKRISLPEMSEEELNESI
                     KFEAEQYVPFDIEDVNLDFQILGPKEEAGQMDVILVAVKKDVINEYVSVVKEAGLNAV
                     IVDVDAFALENMYGMNYEIEAGRNVALVNIGASTMNMNILRGGVSVFTRDSSIGSNMH
                     TDALQKEFNVTYEDAERLKKGEPVEKVKPQDAYALIIESSNDIFSEVSRSLEYFRSSA
                     VHAEVNEVILSGGCALVRDFTRLLSQKIGVEVRAADPFRNIAIPKKIDSSTLEEVAPI
                     AAVAVGLATRRPGDR"
     gene            16035..>16520
                     /locus_tag="VMU21_11455"
     CDS             16035..>16520
                     /locus_tag="VMU21_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HUO78178.1"
                     /translation="MIRVNLLPVKRKKKAKPLPAFVIATTLITVLVICALAYLFYYYN
                     STLQATQNRFDSNKQKIAELKEKIKEVDNFEKLNKTIEDRTKIIEQLRKNQSIPVMML
                     DEISKSLPKGVWLNSMEVTGGGSNVSIDGYAFTNPDVVTFVENLKSSKLLTEIYLQES
                     RQ"
BASE COUNT         4531 a         3725 c         4636 g         3628 t
ORIGIN      
        1 gaatattatt gtttcccgcc gggctgccat agaagaggaa aggcagaaaa agaaagtgat
       61 aacccttacc aagttgaaag agggctcgcg actggaaggc gtggtaaaga acatcaccga
      121 ttacggtgtc tttgtggacc tcgggggtat tgatggtctg ctccatatat cggatatctc
      181 gtgggggagg atcactcacc cctcggagtt ttttgcggtg ggtgataccg ttgaggtcat
      241 tgtgttgaag tatgacgagg aactggaaag ggtgaccctg ggatataagc agaaaaaaat
      301 tgacccatgg ttgtcagtgg gtgaaaaata tccgttgggg gcaaaggtga aaggcaaggt
      361 ggtgagcatc gctgattacg gagcatttat tgaactggaa gaaggggtag agggacttgt
      421 ccacgtctct gagctggact ggtcagcaag gccgaagcac ccgtccaaat acctttccat
      481 cggtgaaagc gttgaggcag ccgtcctcag ggtggacatg gaagagaggc gaatatcgct
      541 cagcattaaa cagttaaagc ccagcccatg ggaactcgta tctcaacggt acagtgtcgg
      601 tcagaagatc tcaggcaaag tgagaagcat taccgaattc ggcgcttttg tgggcctccc
      661 tgaaggggtt gacgggcttg tgcacatatc ggacatttca tggacaaagc atataaaaca
      721 tccctcagag ctgctcaaga aaggtcagaa ggtggaggca gtggttctga gccttgagcc
      781 agagaaagag agaatcgcac tggggatgaa gcagatgcag gctgacccgt ggatcaagga
      841 catacccgaa aaatacaagc ttggtgacga agtgaggtgc aagattttga gacttaccga
      901 tttcgggatt tttgcggaga tcgaaggcga ggttgagggt ctgatttact cgtctgaagt
      961 ggtgaagggt gaagaactgt tgaaggaggg agaggaagtc ttggtgagga taataaagat
     1021 cgatacagaa gacaggaaga tcggcctcag catgaaacat gtgaaaggtg atcagggatg
     1081 aagaaggttc tgctcctgct tttgggactt ctcactgtcc tggtgttcct gagccttatg
     1141 attgcacttc tcttcagggg aagccctatg ggtgacaagg tcgccctcgt acgtatcgaa
     1201 gggcccatcg ttgactcgaa gaaccccatc gacgaaatta aggaatatat gaaggaccct
     1261 tcggtgaagg ccattgtatt gagagtggac agcccgggag gtgcggttgc cccttcccag
     1321 gaaatatacg aagaggtgag aaaagcggta gcgaaaaaaa agatcgtggt ttccatgggg
     1381 tcccttgcgg cttccggcgg ttactatatc tctgcacccg cgtcaaggat tgtggcaaac
     1441 cccggcacgc ttaccggctc catcggggta attatggaga ttcctaatct cgagggcctt
     1501 atgaataaag tgggagtgaa gacagaagtg atcaagagcg gcagacataa ggatattgca
     1561 tcggtattca ggggcatagg ccgtgaggaa agggagatac tccagggcgt gctggatgat
     1621 gtgcatgatc agttcattaa tgcggtggct gaagggagaa agatgttgcc cgatcaggtg
     1681 aaaaagcttg ccgacgggag aatattcaca gggaaacagg cagtgggtat agggcttgtg
     1741 gatgagttgg gaaaccttga ggatgccatt aaggtggctg caaaactttc aggaataaaa
     1801 ggtgaaccgg gtgtggtctc aaaaaaggaa aaattctcct ttatggatat cgtgagggga
     1861 aatttaccca aggagttatc agatatcttt ccgactgtaa aattaaaata ttacttttca
     1921 ccatagagaa agggtaagga taaggtcaag aaatctctgt cttaacctga cccttggatc
     1981 ttgaaaattg aagaggagga tgtatgacaa aatcagcact gatcgacaag gtggcggaaa
     2041 aggtggaagg ccttaccaga aagcagaccg agatcattgt agacactgta tttgatggga
     2101 tcagggatgc cctggcaaaa ggcgaaaaga tcgagatacg agggttcgga aacttcagac
     2161 tcaaacaaag gagaccgaga cgtgcgagga accccaagac aggggaagga gtggatgttc
     2221 cctcaaagag ggttctgcat ttcaaggtgg gcaaggcact gaaggacgcg ctcaaccccc
     2281 aatagagcag gtgattgatt ctatttcacc cggaattatg tagtcgcatt gttctgtcca
     2341 aaggctgcag catactgcgg tggcccttca ttttgctggt gttttcgcct acaatgtgtg
     2401 gtaatacatt tcactgattc ctcttgagcg attccgttgt tcattgcata tcgtgagctg
     2461 tctttgtttc ggcagaggca tccgtctccc aggcaagaca atagcccccc tcggccgcaa
     2521 gggggggcag gcaccttaca acactctgtt ccctcgtatt gtcaaagtcc gctcttgcgg
     2581 acaagcacat aacccttccg cctcaggcgg attgccattt tcgccccgcg aatcagccga
     2641 ggctgactag ccggatacct ctgccgaact tatgaattat cagagctgat atgatatggg
     2701 gcctcacagg tgtaggcata tttgacacgt ccctgcattt ctgataagaa taattcatgt
     2761 gggaatatac agaaaaggtg aaggattttt tcctccatcc aaagaatgtg ggcgagatag
     2821 agcatcccga tgcggtaggt gaagtgggca gcatgctttg cggagacgcc ctgaagctca
     2881 ccctgaagat tgacaaaaaa acgggcagga tcattgatgc aagattccag acctttggtt
     2941 gtgcctctgc catcgcgagt tcgtcggtgc ttaccgaact tatcaaaggc aagacattgg
     3001 atgaagccct cgagctcacg aaccaggata ttgcggaata ccttggcgga ctgcccaagg
     3061 aaaagatgca ctgttccgtg atgggcagag aggccctgga ggcagccatc accaattacc
     3121 gcggggaaac cagacctgtg gagagggaag agaaggtcat ctgcaagtgc tttgaggttt
     3181 ctgaagaaaa gattcggagg gtggcattgg aaaatcatct ttccactgtg gaagagatca
     3241 ccaactacac aaaagcgggg ggaggatgcg gctcgtgcat ccctgccata gaggcaatcc
     3301 tgaaggatat ctggtccgta aaacctgagt gtgaagcgcc caaagcccac aggaagatga
     3361 ccaacctcca gcggattgcc cttattcagg atgtgataga aaaggagatc agaccgcgac
     3421 tccagacgga tgggggagac attgaactca ttgatgtgga tggcaagagg gtgatcgtct
     3481 ccctgagggc gatgtgcgtt gcgtgcccga tgagtgacgt gacagtgaaa ggtatcgaag
     3541 acaaattgag agagctggtg agcgaagacc tggtggtgga agcggaatga aaccaatcta
     3601 ccttgacaat aatgcgacga ccgccgttgc ccctgaggtc tttgaggaaa tgcggcccta
     3661 tctccaggaa ttctacggaa acccctcgag tatgcacaca ttcggcggcc agctttacag
     3721 gaagatagag gaggcgaggg agaaagttgc tgccctcatc ggcgcagtgc ctgaagagat
     3781 catctttacc agctgtggca ctgaaagcga taataccgcg ctcatgagcg ccatagaatc
     3841 atatcctgcg aagagacatg tcatcacgac gagagtggaa caccctgcgg ttctgaactt
     3901 ttgcaaacac cttgcaagaa aaggctatag ggtgacgttc cttcctgtgg atagcttggg
     3961 aaggctggat atggatgcct tttccagggc agtggatgac gataccgctg ttgtgtcggt
     4021 aatgtatgcg aataacgaga cgggtgtggt attccctgtc agggagatgg gtgaagtcct
     4081 gagagaaaga aaaatccttt ttcatacaga tgcggtgcag gccgtgggta agatccccat
     4141 agatacaaga accctcccgg tcgacatgct ctccctttcg ggacataagg tgcacgcccc
     4201 caagggggtg ggagtcctct atgtgagaaa agggacccgg ttttatccct atatcatcgg
     4261 tggacatcag gaaaggggaa gaagggcggg aacggaaaat gtggcctccc tcatcgccct
     4321 cgggaaggca tgtgaacttg cggggtcagc cttccgtgga gaggtcgact atatgggcag
     4381 actaagggac aagctcgaaa aggcgttgct ccagtgctgc cctgatgtgc gggtgaacgg
     4441 agataccgaa caccgtttgc ccaataccgc gaatataagc ttcgagtatg tggaagggga
     4501 agctatcctc ctcagaatgg atgaatacgg catctgtgcg tcttcaggtt ccgcatgcac
     4561 ctcaggctct cttgaaccga gtcatgtgct gaggaccatg ggggtatctt ttaccgcgat
     4621 ccatggatcc atacggtttt ccctcagccg gtataccagt gatgctgaga tagacagggt
     4681 gattgaggtg atgcccggca tcatcaagga actgagaggc ctctctccgt acgggagaat
     4741 aaagatggtt tccggttaag accgaagtac gttctttttc tttctttata cctccgtgtg
     4801 ctctgctcac ctgcagagca acagcgtata ttccgcagtg catactgcat gaagggtgag
     4861 tatccccgag gattcatacg ttcccttcca ttcctttttg gaaaaaagtg cttattttat
     4921 gttaaattaa aaaatatgga gacacgatga ttaagaccgt tgaagatata agtgcaacaa
     4981 ggaaacgcct caggatagaa atccccgcag acatgattga aaaagagata cgggattccc
     5041 tcgaaaagct gagacgtaag acgaccatcc ctggattcag gaccggcaag gcgcccatta
     5101 accttattga aaagaggttt ggaaaagagg ttgagtccga tgtgctcgaa cggatcattc
     5161 ctcaaggcta cgtggaagcg ttgcgggagg caaacatcac ccctgttgca agccctgtgc
     5221 tggaagagaa gagcgatttt aagagaaggc agcccatctc catgaccctt atggtggagg
     5281 tgatgccgaa tatcgaaaat ctcaaatatg aggatatacc actcaaggat atccccgtta
     5341 cggtgagcga ttcagacgta gaaagtgtcc tcaagagaca gcaggaagag aaggcaacgt
     5401 acgagccctc tgaaggtcct gttgaaatga atgaccttat tgtggtggat tattcttctc
     5461 ccggagatgg cgtggaagcc aaggatcaga tagtgaaggt ggggggaagc atgtttcctg
     5521 aagagttcac ccggaaactc gttggaaggg ggaagggtga agagctctcg atcgaagcca
     5581 cattcccggg ggatcacccg ttggagaatc ttgccgggaa acacctgacg atgaaggtga
     5641 tgattaagga tatcaagaag gtgaatcttc ccgcagtcga tgatgagctt gcgaaggaca
     5701 tagggtttca gaacctcgag gaaatgagaa aacgcattgg tgaggagata gaaaaggcga
     5761 aaagaaacga ggttgccaag atacaaaagg cggagatcat caggaacctt gtggagtccc
     5821 acgagtttga tatccctgaa tccctggtgg aacgggagag cgaaatgctc gcgtcagcgg
     5881 ctttgtccca tagaaccgag gcggcagagc cctctgagca tgagcttgat gcgcttaaac
     5941 aggaggcacg ccccaatgct atgagaaatg tgaaggcatc actgctcatt gaaaccatcg
     6001 ggaaggcgca gggggtttcg gtggcagaag atgaggtaaa gcgcgcgatc atttcccttg
     6061 cacagagact ttctgtttca cccgaacaga tcatgaagtt ttatatctcc cgggatggat
     6121 ccctggaggg cctcaagggt tcgttgtttg aggagaaggt gcttgacctc atactctcga
     6181 aggcgaaact ggagaaagga gaatagcgtg agcgtaatac ctatcgtaat tgagcagaca
     6241 ggacgttctg agagggcgta tgacatatat tcaaggcttc taaaggacag gatcatcttc
     6301 atgggaacgg caattgacga tgcggttgca aacaccgtaa tcgcgcagct tttgttcctg
     6361 cagactgagg accctgacaa ggatatccac ctctatgtca attcgccggg cgggatcgtc
     6421 tcttcagggc ttgccattta cgataccatg cagtacgtga agtctgatat tgccacctac
     6481 tgcattgggc aggcgacgag catgggggcg ctgctgctgg ctgcgggaac ccaaaagaag
     6541 aggttcgcgt tgccccattc aaggataatg atccatcagc ccatgggtgg ctttcagggt
     6601 caggccaccg atatcgagat ccacgccagg gagatactca gaaccaagga tatcctcaat
     6661 cagatcctcg catcccatac gggtcagtct attgagaaga ttcggacaga cacggacagg
     6721 gactttttca tgtcaggaga agaagccaag gagtacggta ttgtggacga ggttatccat
     6781 agcatgaaga agaagtgaac ggaggagagt agaatggcca agaaagaaaa ggacgaaacg
     6841 aaactcaaat gctccttctg cggcaagggt caggatgagg tgaaaaaact catcgcaggg
     6901 cccacggtct atatctgcga tgagtgcgtg gagctctgta acgagatcat cgctgaggaa
     6961 ctctatgcag aaaccacaac ccatacactg cccaagctcc cgacacccaa agagatacac
     7021 gaattcctga atgattttgt gatcggtcag gaaaaggcca agaagatact ttccgtagca
     7081 gtgcacaatc actataaaag gatctacagt ccgggtgctt ctgacgtgga actgcagaag
     7141 agcaacatcc tccttatcgg cccgacggga accggcaaga cacttcttgc ccagaccctc
     7201 gcaaggctgc tcgatgtccc cttcaccata gcagacgcaa ccacccttac cgaggcaggg
     7261 tacgtgggtg aggatgtgga gaatatcatc ctcaaactcc tccaggcatc ggattacgaa
     7321 gtggagaggg cacagagggg gatcgtgtat attgatgaga tcgataagat cagcagaaag
     7381 tcagaaaacc cctccattac acgggatgtc tccggtgaag gagtacagca ggcccttctc
     7441 aagatcatag aggggaccat ggccagtatt ccgccccagg gagggaggaa acacccccag
     7501 caggagttta tccaggtgga taccaccaac atcctcttca tatgcggcgg tgcttttgtg
     7561 ggactggacg gcatcgtgga gcagaggacc ggcaagaaga cccttggatt tggcgcagag
     7621 gtcatcggtg ccaaggagag gaagatagga aatgtgctga gccttgtgga gcctgaggac
     7681 ctcatcaaat acgggatgat tcccgagttc gtggggaggc tcccggttgt tgcgctcctt
     7741 gatgaacttg atgagggtgc gctggtgagg atactcacag agccaaagaa ctctctcatc
     7801 aagcagtatc aaaagctgct ctcgctcgac aatgtgcggc tcagattcac cgatactgcg
     7861 cttgccgcga tctcgaagcg tgccatacag aggaagacgg gcgcccgggg actgcgcgcc
     7921 atcctcgagg aggtcatgct tgacgtgatg tacgagattc cttcccagaa ggcgatcagg
     7981 gagtgtctta tcaatgagga tacgatactg aaaagggaac gtcccatcct gatttacgaa
     8041 aaacaggcag aatcagcata gccgaatact taggcttttc acgagcacac ttgcataaag
     8101 ggccctgcag tacgctgcag ggccctttat ttcgtggtgt caggtcaggg gaagcgggaa
     8161 gagccaccgc acagtgcagg tgttatgcgg ggtccttccg ttaatcttca ctgttgcaag
     8221 taattgaagc agacttgtta gtcgatggaa tcgttaaaac aattcaaaga gagcgcacgt
     8281 aaagacgcac caggaagaaa actgtgtgtg ctgaggaata taatgtcttc catcgtataa
     8341 acagttcagg tcttatcggg tgattttatt cttaatatcc ttcaccgcca gattattcaa
     8401 aagtctcata aatttcggat ttgctacaag tgcggagaca tcgcctgctt gcaccgcctt
     8461 catgatttca gggtcttgaa gaatttcttg aaattctgga tcgttctgca gaccctgcat
     8521 catattcatg atctcaccat cactcatcat cttgttttga agagacatta tttccccccc
     8581 ggtgtttgca gaagttcccg cagaatcctt caagcggata gtgcggatgt tcgattcttc
     8641 aacccgcacg gttccaaggg tggctgacct gatcgtacag acacctcctg tgagtgaaac
     8701 aatctcgccg gtaaggatgg ttccgtctgt cagctcgatt tctcgcatct cagcggcagt
     8761 taccatacct gcggcgatcg ccagtgtcag gaacgtcaga attaaggatt tcatgctacc
     8821 tcccgatggg attgtcttcg ctccagtcaa aaaaaaagga aaatccagaa gtaaggaatc
     8881 atgctttcac ttttcttgca gagagtgatt tctccatggt cttcctgatg ctctctttca
     8941 gcgggccgtc ttccagtccg gaaaccgtct cttctatctg ctgcagagaa tagcggtcaa
     9001 gggggatttt tttctgcggc gggtttgggg gtgcaccttt tgttttcgtg cgcaccgttc
     9061 ctaccctgaa cctcacgtcc tttaccccga agggactcac gttcttgagt atctcgcctt
     9121 tgaggaagat gagctgctga tgccataccg gtgaatccac tgtgataagg agctcaccat
     9181 gcttcagact gcagggggac gtgtggaggg ataagggctc acgaaatata tcggaccact
     9241 ctttctttat ccgttcgaac ctcaccgctt cttcgagccc cagtcctgcg acaagggggg
     9301 agatgagatt cccgactctc ttcacaaggc ctttttcacc aatccggacc gcaaacaccg
     9361 tgtacagaca tattcgcgat gtaccccctt attgatcata atgcgagtcc tctgaaggtt
     9421 gggcaggacc tgacgctttg tcctgttgtt tgcatgactc acattgttcc ccattaccct
     9481 tcctttaccg cacacaaaac atgttgccat tggtgtgtcc tccaattcgt gaatgtagta
     9541 gtatctggac tgttcaagac agtcagggat tcgggaagcg ggaaaatcag ctgaggcttg
     9601 actccgcgaa tcctgtgcaa gtccacaggg acaattgcat aaaagaagtt ttttatgata
     9661 acattttagt ctaacggtta caagggcacc ttacctgaag gggagcgtga gggcagtcac
     9721 ggtatggtct tcttgcatcg gaatggaggt ttgttgagat gtcaaaactg aggatctacg
     9781 aactcgcgaa gaagctgaac gtcacccaca aagaagtaat ctctgaactc ttaaggctcg
     9841 gcatatcggt caaatctcat gcttccaccg tagaggacga gatcgcggga aaagttgagg
     9901 ctattttcag gaaaaaacag aagcctgctc ctgtcgctaa gccgaaggcg tcaaaaccaa
     9961 aggaagctcc ggtaaagaaa ggaaagcctt ccgagattgt tccaaagccg gtcaaaatcc
    10021 aggaagcccc tgttaaggca ggaaggcctc cggtagtacc cccgaaggct gaaaaaccca
    10081 aaatagcaga accaaagatt gagaaacccg ttgcaccacg cgccagagag gagaaggtag
    10141 aaaagtccgc agtgccgatc gagaagcctg aagagaaagt agaggctgaa aaaaaggaag
    10201 aggaaaaaga atatgtctcc cctgcggcag agggaaaaac ccttgaggag ccaattctcc
    10261 ccatagaaga ggaagacctc aaggttcctg accgattcaa gaaggatatc gagacggaaa
    10321 aggtggaaaa attcaaggtg aaacctgcga tgcagcgtgc atttcaggca gtaagaaaga
    10381 tagagccgaa gaggtggcat gaagtcaaga cattcaagaa gcctggcagg gatagagggg
    10441 tgcagaagat cgcggaaaag ccccagcccc aggtaacggc gccgaggaaa aagatgttga
    10501 agatccagga agggaccacg gtgaaggaat ttgccgaact gatcagtgtg aaactccctg
    10561 acgtgataaa gaagtttatg gaactgggag atatgccgac catcaatcaa cccgtggata
    10621 tggacgctgc gcttcttgtt gctgaagggt tcggggtgaa gctggaggtt gctgcgggag
    10681 aagaggaaac tgtaatcgag gaaacggcgg aggacgccac ttccctctcg ccaaggcctc
    10741 cggtggtgac catcatggga catgtggacc acggaaagac atccctcctc gatgccatca
    10801 gggagacaaa ggtcaccgaa acagaggcgg gcggtattac ccagcacatt ggcgcctata
    10861 aggtgaccct gaagggaaaa gagattgtct ttctcgatac gccaggccac gaagcattca
    10921 cttcattaag ggcgagggga gcgaaggtaa cggatgtcgt ggtgcttgtg gtggctgctg
    10981 atgacggcgt gatgccccag accatcgagg caatcgacca tgcgaaggca gcgaatgtgc
    11041 ccatcgtggt ggccatcaat aagatagaca aacccgaggc aaatcccacc aaggtcaaaa
    11101 atgagctctc ggcgcgcggt atcgtctccg aagagtgggg aggacagaac atctttgttg
    11161 aggtttccgc caaaaagcgt atcggcatag aaagccttct ggagatgatc ctcctccagt
    11221 ccgatatcat ggagcttaag gcaaacgcca agaaaatggc aaaggggacc atcatcgaag
    11281 caaggctgga caggggaaga ggtccggttg ccacggtcct tgtgcagaac gggaccctca
    11341 ggatcgggga tgtcttcctc accggaatca gcgtgggaaa ggttcgggcc ctcatcgatg
    11401 atacagggaa ccggatctct gaagcgcagc cggcaacgcc cgtggaggta ataggttttt
    11461 cagaggtgcc cactgccggc gatctcttta ccgttgtgga tgatgagaaa aaggcccgcc
    11521 agatcgcttt ggcaagacaa caaaaacagc ggctggccga gatggccagg gcgaaaaagc
    11581 tgaccctcga cgaagtctat gcaaaaatta aagaggggga gataagagaa ctcaatatta
    11641 tcataaaagg tgatgtgcag ggatcggttg aggcgctgaa agaggcattt gagcagatta
    11701 tacatcccca ggtcaaggta aaggtgatcc acacctcggt gggcggtatt aatgaatctg
    11761 acgtcatgct cgcagcagcc tccaacgcca tcattatagg tttcaatgtc aggccggaat
    11821 cgaaggccca tcaggtagca gagaaggagg gtgttgacat acggcactac aacatcatct
    11881 atgaagccat cgatgacatc cggaaggcac tggagggact cctggagccg acgctgaagg
    11941 aaaagatact gggcagggca gaggtgagac agaccttcca ggtatcgaga ctggggacta
    12001 tcgcaggctg ttatgtggtg gacggaatca taagccgtac cagcgacggc ataaggatta
    12061 taagggacaa catcgtggtg tatgacggga agttgggttc tctcaagcgg ttcaaggagg
    12121 atgtgaggga agttcagaca ggatacgagt gcggcattac gatagagaac tacaatgatt
    12181 taaaggtcgg cgacgttctc gaaaactatg tcatagaaaa aatagcggcg aaactgtaat
    12241 acaaaacgcg cattactggg gaatgaagtg ccccgaaacg caatagggat gcgctccctg
    12301 tccatgtcag gagaacggag ccaagaagcg aatctgaaga ccgtctcttt ttttcatttg
    12361 acctctgagt actaaaacga gatactgacc gcgtattccg caggcgaaag gagaatatgc
    12421 ttccttataa gcgttcacag agggtaagcg accttctgag acaggagatc gccgacataa
    12481 tcatgagaaa ggtgaaggac ccccgcctcg gctttgtcac agtaacaggc gttgatctaa
    12541 ccgacgacct caagatcgcc agggtgtttg tgagctgcct caaggaggaa gaaagggaaa
    12601 ctaccttgga tatcctgaat gcggcaaaga gcttcatacg ttctgaggtc gggaaaaggg
    12661 tccggatgaa ggtgctcccc tctctcgagt tcagggtgga tgaatccgta gggtatgggg
    12721 acaggatcga gaagctgctg agggagatca agggtaacac ataaattgaa tatccctgac
    12781 agacttatac aattcatcct tcaggaacgt aagtttttta tcgcaaccca cattcgcccg
    12841 gagggtgatg cccttggttc atccctggcc ctctctctgg ccctggaatc catgggcaaa
    12901 gagaccaccc tctacgagcg tgacccggtg ccggcgcttt accggttttt gccgggttat
    12961 aagaggttca cgcacaccca ctcgcccctc gcacaggacg ggctccccct ccttttactt
    13021 gactgcaatg agcctgaaag ggcgggtctt gagaatgtgg tcatccgtcg ttctgcggtc
    13081 atagaccacc atgagacaat aaaggagttc ggggacattc agtggataga gcctcatgct
    13141 gcggcaaccg gggtgatggt atacgacctt atcaggaaac tcgggattgg gattacgaag
    13201 gatatggcga cgaacctcta tgccgccatt gccactgaca caggcacctt cagattcagc
    13261 aatacctcat cagatgtcct caggatcgcc gcaggattgg tggaatcagg ggctgatccc
    13321 ggagtcattg cgggagaatt gtatgagaca tggtccaagg ggaggttcag tctcttgaca
    13381 aaagtactga ataccctgga actcacccat gggattgccg tgactgtggt aacaaaagcg
    13441 atgttctcgg aaacagggac ttcccctgag gacactgaga atttttccaa tttccctaag
    13501 atgattgaag agattaaggt ttcgctcttc ctcagggaaa tagatgaggg gtggaaggtg
    13561 agcctccggt ccaagggcga ttgtaatgtt gcagagatcg ctgtgcggtt ccatggcgga
    13621 ggacataaga acgctgccgg gtgcgtgatc aaaggagatt taaagaccgc aaaaacccaa
    13681 ttgataaaag cgatagaggg gatcaaggga taaagaggaa aagtgaaaat tcctggaaga
    13741 caggccttga atcccaaatc cccgaattcc tgatcccctt ttccacggtc acatgaacgt
    13801 tgtcatcaat ctgaacaagc caaaaggcat atcatcgcaa caggccgtta cgcgggtcaa
    13861 gaggatcacg ggagtgagaa aggccgggca cgcggggacc cttgatccca tggcgacggg
    13921 catattgctt gtctgcaccg gggaagcgac caaaataacg agattcctct ccgaccttga
    13981 caaggaatac ctcaccgtga tgaagctggg tgagaagacg gatacctacg attctgaagg
    14041 gaccgtggtg cagaggagtg aggggttttc attggggaag gaggatatcg aaaaggtcct
    14101 cgcaggattc aggggtacca ttgaacagac cccacccatg ttttcggcgc tcaaggtggg
    14161 cggccggccg ctctacgccc ttgccagaag agggataacg gttgagaggg caggcaggga
    14221 gatcactatt catggaatcg aaatcaccgg attcagtctc ccgttcgtcg ggatgaaaat
    14281 tacgtgctcc aagggtacct acatcaggtc cctctgcaac gatatcggag aggctttggg
    14341 gattggtgcc catatcacgg acctgcaaag gacgcggata ggagggttcc gcattgaaaa
    14401 ttccgtgacc attgatgacc ttgagaaatc ccctgctgct gcaagaaatg catttgtgag
    14461 tgttgatgaa gcgctgggac atctcaggga aatatccctt gatgagggag atttcaacag
    14521 ggccaggaac ggcctatccg tcaaactcta tgcgtccggt ctcccgcaag aaagctttgt
    14581 tcgtttgaag gacccttcgg gtcatctttt tgccctgggc agggttcttg agaacacggt
    14641 gaagatagag aggatccttc acctttgctg acgtaacacc ttttgatcag acaattaatt
    14701 gacaaaatct gtcaagaata ttttataatt cccaggttag cccattgcaa gcagcacgag
    14761 tgtggtcgct ttcccttcca tgaaatacat actggccgct ctagaaatta gcatgatcac
    14821 ttgagaaaaa tcgggcatga aaactgcttt cacgggaaat ttgttggatg gagatggctt
    14881 aacacatgca ttatttaaac cattgtttta aggtttattt tggaacctgt tatttttaat
    14941 gaaaaaaata cttgacaagt ttatcttttt ctggtattca ttaagctgat gtttggtaaa
    15001 aaaggcataa taggaattga cattggctcc agctatatta aggcggtaca gctgaacgag
    15061 acccgttccg gctttgagct tgagctgttc gacatgcttg cgctcccccc cgagcttatt
    15121 gtggaaagct ccattattga tgccatcagg gtaaccgaat ccctcaaaga gctcgtacgg
    15181 caggccaaaa ttaaggcgaa ggatgcggta atctccgtct ccggccactc ttcagtaatc
    15241 attaagagaa tctccttacc tgagatgtcg gaggaggaac tgaacgagtc cataaagttt
    15301 gaggcagagc agtatgtccc ctttgacata gaggatgtga atctcgactt ccagatcctg
    15361 gggccaaagg aagaagcggg acagatggac gttattcttg ttgcggtgaa gaaggacgtg
    15421 atcaacgagt acgtctctgt ggtgaaggag gccggtctga atgcggtaat cgtggatgtg
    15481 gacgcctttg cccttgagaa catgtacgga atgaactacg agattgaggc ggggaggaac
    15541 gtcgcccttg tcaatatcgg agcaagcacc atgaacatga atatccttag gggcggcgta
    15601 tcggtcttta ccagggacag ttccatcggg agcaacatgc acaccgacgc gctccagaaa
    15661 gaattcaacg tcacctatga agacgcggaa cgcctcaaga agggggagcc ggtggaaaag
    15721 gtgaaacccc aggatgccta tgcgctcatt attgaatcat ccaacgatat cttcagcgag
    15781 gtgtcccgct cccttgagta tttcaggagc tccgcggtcc atgctgaggt aaacgaggtg
    15841 atactcagcg gaggctgtgc cctcgtcagg gatttcacga ggctcctttc acagaagata
    15901 ggggtagagg tgagagcggc tgacccattc aggaatatag ccatccccaa gaagattgac
    15961 tcttccactc tggaagaggt agctcccata gccgcagttg ccgtcggtct tgctacacgg
    16021 aggccagggg acagatgata agagtcaatc ttttacccgt caagcggaaa aagaaggcaa
    16081 aaccacttcc cgcctttgtc attgccacta cactgattac cgtgctcgta atctgcgccc
    16141 tcgcctacct cttttactat tacaactcga ctcttcaggc cacacagaat cggtttgact
    16201 caaacaaaca gaagatcgcg gaactgaagg aaaagataaa ggaagtggac aacttcgaaa
    16261 aacttaacaa gaccattgaa gacaggacaa agattataga gcagctcagg aaaaaccaga
    16321 gcattccggt aatgatgctg gacgaaatta gcaagagcct ccccaaaggg gtctggttaa
    16381 attcaatgga ggtgaccggg ggcggcagca acgtgagcat cgatgggtat gcctttacca
    16441 accccgatgt agtgaccttt gtggagaacc tgaagagttc caaactgctg accgaaatat
    16501 acctgcagga gtcaaggcag
//
LOCUS       DATKOU010000241         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4957572, whole genome shotgun sequence.
ACCESSION   DATKOU010000241 DATKOU010000000
VERSION     DATKOU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4957572"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..572
                     /locus_tag="VMU21_11460"
     CDS             78..572
                     /locus_tag="VMU21_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HUO78179.1"
                     /translation="MAEFDLVMYEEEFKRIDEELQKLHQQANAKVLFLVDKNGQLIAS
                     AGDTHNIDTTALASLTAGNIAATGGIARLLGEREFTILFHEGEKDNIHISLIGQRVIL
                     VVIFDQRSSLGLVRLRVKKASESLTRIFDEISRKAEKEKVEGRLDESPFAEISDEDID
                     NLFR"
     gene            583..1170
                     /locus_tag="VMU21_11465"
     CDS             583..1170
                     /locus_tag="VMU21_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HUO78180.1"
                     /translation="MSFINYSSREINCKIVYYGPGLCGKTTNLQYIYRRTTPEQKGKL
                     ISLATETERTLFFDFLPLALGDIKGFRVRFHLYTVPGQVFYAASRKLILKGVDGVVFV
                     ADSQIERMEANMESLEDLKINLAEQGYDLEKLSFTIQHNKRDLPNIAPLDEMNTILNP
                     NGIPWFEGVAMTGQGVFETLKNVAKQVLFELKKNY"
     gene            1199..1516
                     /locus_tag="VMU21_11470"
     CDS             1199..1516
                     /locus_tag="VMU21_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUO78181.1"
                     /translation="MPKYPRNYMMLQKKFPDLMKAHEEVGRLAREAGPLNAKTGNLIQ
                     LAASVALRSEGGVHSHAKRAIQAGASRDEIYHTIALLMNTVGFPTIAAAFSWVNDIVA
                     KKK"
     gene            1638..>3374
                     /locus_tag="VMU21_11475"
     CDS             1638..>3374
                     /locus_tag="VMU21_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO78182.1"
                     /translation="MENDFTIRPGTSAQVKGQPKLGRLLVVDDQVDVLNPLCEILSKF
                     GYDATGVTSASEALKELKNQSFDLLLTDLIMPEMDGVSLLKTASEMDPQIVGIIITGN
                     GTIQTAVEAIKGGAFDYILKPVEFRTLMPILSRAMMVRRLRIENLQLRESHKALIESA
                     RDVIYTLSPGGLVTSLNPAFEIITGWSRAEWIGKPFPSILHPDDVPSALKVFYGALRG
                     EVSPTLELRVRARSGEYLVGEFIMPPLIADGKVIGISGIARDITERKKAEEKLRALME
                     ELSTQKEFSEAIYNNTPSGIIVLDKEGHILKINHPGAEILKISPSEVVGRPLTAIYPE
                     AKEMLTLAAHFGREFLITLADGRTRPVGFTNSPLLDKGGMEKGIIIVFRDLTEVKELQ
                     AELRKKQHFEAMGKVISGVAHEIRNPLFGIQAIGQILERETETPQHQALISALLKETC
                     RVRNLIDELLLYSRPSVLNFIEMDLDIFLSELKEFFKTKNYDAVLSISVPPLVRFKAD
                     KDKLMQVFVNLLENAVGAGSKRIEITSEKRDGGLRIAVKDDGAGIQPDALERIFEPFF
                     TTKKEGTGLGLPI"
BASE COUNT          917 a          802 c          872 g          783 t
ORIGIN      
        1 ccttcaaaaa actgggggat ggcaagccgg ggaatagtat tgatataaat ataataatgt
       61 gctatttttg aggtgctatg gcagaattcg accttgtaat gtacgaggag gagttcaagc
      121 gcattgacga agaactccag aagttacatc agcaggccaa cgcaaaggtc ttgtttttgg
      181 tggacaaaaa cggtcagctt attgcctctg caggcgatac ccacaatatt gataccaccg
      241 cgcttgcatc gttaactgcc gggaatattg ccgccaccgg cggaattgca aggcttctcg
      301 gtgaaaggga gttcaccatt cttttccacg aaggggagaa ggacaatatc cacatctccc
      361 ttatcggtca gagggtcatc cttgtggtga tcttcgatca gaggtcatca ctggggctcg
      421 tgcggttacg ggtaaaaaag gcatccgagt ctctcaccag gatctttgac gaaatatcga
      481 gaaaggcaga aaaggaaaag gtggagggca gattagatga gtctcccttt gcagagataa
      541 gtgatgagga catcgacaac cttttcagat aggtaagctt ccgtgtcttt tattaattac
      601 tcctcccgtg agattaactg caagatcgtc tattacgggc cgggtctctg cggaaagacc
      661 accaatctcc agtatatcta caggaggaca acccctgagc agaagggtaa actcatatca
      721 ctggccacag agaccgagag aacgcttttc tttgattttc ttcccctggc ccttggagat
      781 atcaagggct tcagggtaag gttccacctg tacacggtcc ccggacaggt cttctatgcg
      841 gcgagcagaa aattgattct caagggtgtt gacggtgtgg tatttgttgc agattcccag
      901 atagaacgga tggaggccaa tatggagagc cttgaagacc tgaagatcaa tctcgcagag
      961 cagggatacg acctggagaa actctccttt acaattcagc acaacaaaag agacttgcct
     1021 aatatcgcac ctttggatga aatgaacacg atcctcaatc ccaacggtat cccctggttt
     1081 gagggtgtgg cgatgaccgg acagggtgtt tttgagaccc ttaagaatgt ggcaaaacag
     1141 gtgctcttcg aactgaaaaa gaactattga gagaaagtga ggggagcaag gaggacatat
     1201 gccaaaatac cccaggaatt atatgatgct ccagaagaaa ttccctgatc tcatgaaagc
     1261 gcatgaagaa gtgggaaggc tggcgaggga agcgggcccg ctaaatgcaa aaaccggaaa
     1321 cctcattcaa ctcgctgcct ccgtcgccct gcggtctgaa gggggtgtgc actcccatgc
     1381 gaaaagggcc attcaagcag gggcgtcaag ggatgagatt tatcatacca ttgccctcct
     1441 tatgaacacc gtgggcttcc ccaccattgc agcggctttt tcatgggtga atgatatcgt
     1501 ggcaaaaaag aaatagattc cctgcttcct taacaaaaaa tccgtagctt ttttttagtt
     1561 aatattctat attattttta ttagtatcgt tgtttcacat actacaaatc ccgcacttac
     1621 ataaaagacc tatggaaatg gagaatgact ttacgataag gccggggaca agcgcacagg
     1681 tgaaggggca gccaaaatta gggcgcttgc tggtcgtgga cgatcaagtt gatgttctca
     1741 accccctctg cgagattctg tcaaagttcg gctacgatgc cacgggggtt acctctgcct
     1801 cagaagcact gaaggagttg aagaatcagt cctttgacct gctcctgaca gacctgataa
     1861 tgcctgaaat ggacggtgtt tcccttctca aaaccgcttc ggaaatggat ccccagattg
     1921 tgggcatcat catcaccggg aacggtacga tccagactgc tgtggaggcc ataaagggag
     1981 gcgcctttga ttatattctg aaaccggtgg aattcaggac gctgatgccc attctgtccc
     2041 gtgcgatgat ggtacgccgc ctaaggattg aaaaccttca gttgcgtgag agccataaag
     2101 ctctcattga gagcgcaaga gatgttatct acaccctctc tcccggcggt ttggtgacct
     2161 ccctcaatcc cgcattcgag ataattaccg gctggtcgcg ggctgagtgg atcggcaagc
     2221 cttttccctc catccttcat ccggatgatg ttccttctgc gctcaaggtg ttctatggag
     2281 cgttacgcgg cgaagtctct cccaccctcg aattgcgtgt gcgcgcccgc tcaggagagt
     2341 atctcgtggg agaattcatc atgccgccgc tcatcgcgga cggcaaagta atcggcattt
     2401 cgggtattgc ccgtgatatc accgagcgca aaaaagcgga agagaaatta agggcgctca
     2461 tggaggagtt aagtactcaa aaggagtttt ctgaagccat ttataacaat accccaagcg
     2521 gcataatagt ccttgataag gaaggccata tcctaaagat caatcatccg ggagctgaga
     2581 tcctgaagat ctcaccttcc gaggtcgtgg gcaggccact caccgccatt taccctgagg
     2641 caaaagagat gctcaccctt gccgcccatt tcggcaggga attcctcatc actctggcgg
     2701 acggaaggac gaggcccgtg ggctttacca actccccgct cctggacaaa ggcggaatgg
     2761 agaagggaat tattatcgtc ttccgtgacc ttaccgaggt taaggaactc caggcagagt
     2821 taaggaagaa acagcacttt gaagccatgg gaaaggtgat ttcaggggtt gcccatgaaa
     2881 taaggaaccc tctctttggt atccaggcca tcgggcagat actcgagagg gagaccgaaa
     2941 caccccagca tcaggcgctc atcagtgcac tgctcaaaga aacctgcagg gtgaggaacc
     3001 tcatcgatga actcctcctt tacagcaggc cgtctgtgct caattttatt gaaatggatc
     3061 tcgacatatt cctgtcagaa ctgaaggagt ttttcaaaac aaaaaactac gatgcagtcc
     3121 tctccatcag cgtgcctcca cttgttcgct tcaaggccga taaggacaaa ctgatgcagg
     3181 tgtttgtgaa tctcctggaa aacgcagtgg gagcaggcag taaacggata gagatcacct
     3241 cagagaaaag ggacggcggg ttgaggatag ccgtgaagga tgacggagca ggcattcaac
     3301 ctgatgccct ggagaggata tttgaaccgt tcttcactac aaagaaggaa ggcacaggcc
     3361 tcggactccc cata
//
LOCUS       DATKOU010000242         5394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3410695, whole genome shotgun sequence.
ACCESSION   DATKOU010000242 DATKOU010000000
VERSION     DATKOU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410695"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1363
                     /locus_tag="VMU21_11480"
     CDS             224..1363
                     /locus_tag="VMU21_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HUO78183.1"
                     /translation="MLKRYLLAPGPTPVPSEALLAMAMPILHHRSPDFLPILDTAKKG
                     LQWLCQTKNDVLILCSTGTGGMVGSVNNFFSPGDRALVINAGNFGERWTKICNAYKLE
                     VNEIKLDWGNAVKPEDVEKALKKDPSIKGVFVQASETSTGVYHDIEAIASIVKKHEGT
                     ILVVDAISALVAHDLKMDQWGIDVLIAGSQKGLMLPPGLAFVGISEKAWKCADKAQCP
                     KFYFNFKKERESLAKNQTNFTSPVTLIIGLNECLKLLQKEGLENAFERHKRLAHATRE
                     AVRAIGLKLFSRESHSNSVTAIQSPDGLDGQEIYKNLRVKYGITAAGGQGQAKGKIFR
                     IAHLGYADTFDIITAVAGVEMVLKSMGHPLKLGTGVAIAEELLMK"
     gene            1376..2953
                     /gene="serA"
                     /locus_tag="VMU21_11485"
     CDS             1376..2953
                     /gene="serA"
                     /locus_tag="VMU21_11485"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HUO78184.1"
                     /translation="MKVLISDNLSPRGIEVLKQSGLEVDVKTGMKPEELKACIGEYHG
                     LIIRSATKVTAEIVEAAANLKVVGRAGSGLDNVDKAAASKKGIVVMNTPGGNTITTAE
                     HTIALMFALARQIPQATASMKQGKWEKKRFMGVELFKKTIGIVGIGNIGKHVARRALS
                     LGMIVIGYDPYLSDENAKEMGIEKVDLTTLFKRSDFITIHTPITSETKNLISTKTIQL
                     MKDGVRIINCARGGIVNEAELYEALKKEKVAGAALDVFEKEPPGENPLLTLDSVICTP
                     HLGAATEEAQENVAVAIAEQVVDCLVNGVIRNAVNFPSVPAEQVLRLKPYLTLAEKLG
                     TFASQVFEGGVTEITVEYRGEASQLNTAPVNIALLKGFLTPILDETVNFVNAPVITKE
                     RGIEVKEMKSPDGGDYQSMIILRVKADRKEHRLSGTLLGRTDPRIVRIDDFAVEIVPE
                     GTMLFMYNNDRPGVIGNIGSYLGKCNINIARMHFGREREGGMAISVVNIDSPISESQL
                     EDLKKMPNILSVKVVEL"
     gene            2986..4272
                     /locus_tag="VMU21_11490"
     CDS             2986..4272
                     /locus_tag="VMU21_11490"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HUO78185.1"
                     /translation="MASVVIVGAQWGDEGKGKIVDVLTEKADVVARYQGGHNAGHTVV
                     IKNEKFVLHLIPSGILHKDKLCIIGNGVVIEPSSLIDEIKVLKEKGLEIGKNLLISKT
                     AHLIMPYHVAIDNEHERAKGSRKIGTTGRGIGPAYVDKMGRTGIRVVDLYSPQVFKEK
                     LEANLHEVNYLIENRYKAKGFSLDEIFDLYMEYAEKISPHVADTDIIVNDMVRDGKKI
                     LFEGAQGTLLDVDHGTYPFVTSSSSIAGGACTGLGVGPTKITKVLGVAKAYTTRVGGG
                     PFPTEIHDAVGEALRQKGGEYGATTGRPRRCGWLDLVGLRHALRVNGLTGLAITKLDI
                     LDDLEKIKVCVAYKYGKHLHEDLPKEISLFEEAEPVYEEMEGWKEKTVGITTFKELPG
                     NAQHYVRNMEERLGVNVDMISTGQRREELIMLNEQF"
     gene            4273..4788
                     /locus_tag="VMU21_11495"
     CDS             4273..4788
                     /locus_tag="VMU21_11495"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO78186.1"
                     /translation="MRRKSNRVTKRLEVTFSSGGLQFTGISSDLSASGLFIRTQNGLM
                     PGTIVEIEIYLPQHKLGRLKGIVRRTVKTSLSMIKNGMGVELIERDQNYLDFVKSIRG
                     EDKEQREETDAQSGEGKVRQPEGKANETPDSRIIVCVNCETKNRIPLGRVPLGAKCGR
                     CGTLLYLGDID"
     gene            4752..>5394
                     /locus_tag="VMU21_11500"
     CDS             4752..>5394
                     /locus_tag="VMU21_11500"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HUO78187.1"
                     /translation="MWDALISRGHRLTSSLFSAIYPSTCPLCKNSSDTLLYAPICTRC
                     WESMTRYLGPSCRVCATPLVSEYSTLCGECLSHPPPFSMVLNFGLYSGSLSAAIHLLK
                     FSGLKRLAKPLGKLLTELPIPSMDGIVPVPVTKRTLRTRGFNQALLLSVVLSRHLRIP
                     VYMDMLYKKRDTPAQIGLGAKERARNVKNAFEVRGRLDKPRLVLLDDVMTTGAT"
BASE COUNT         1498 a         1184 c         1514 g         1198 t
ORIGIN      
        1 ccgcctaaga gaaagcctat caggaggatg acaatgccca aaaaaatctt tttcttcatt
       61 tatttattat actcgccaat ttatggagtt gtaaagcaaa cttgaccaaa aagcgtttca
      121 taatatatgc taaacccaaa aatcctgcga agcaaaggca tatacatcta aagattgata
      181 ccttgagctc gttcagggta tgtaggaatt taaggagggt tctatgttaa aacgctatct
      241 gcttgctccc ggaccaacgc ctgtaccgtc tgaggccctg ctcgctatgg caatgcctat
      301 tttgcatcac aggtctccag attttctccc aatcctcgat acggcaaaaa aagggttgca
      361 atggctttgt cagacaaaaa acgatgtgct catcctctgc tcgacgggaa ccgggggaat
      421 ggtggggtct gtgaacaatt tctttagtcc cggggacagg gcactggtga ttaacgcggg
      481 gaactttgga gaacgatgga cgaagatctg caatgcctac aagcttgagg tgaatgagat
      541 aaaactggac tggggaaatg cggtgaagcc tgaggatgtg gagaaggcac tgaagaaaga
      601 tccatccatt aagggggtct ttgtgcaggc atctgagacg tctacgggtg tctatcatga
      661 tatagaggcc atcgcctcca ttgtgaaaaa acatgagggg accattcttg tggtcgatgc
      721 catctctgcc cttgtggccc acgacctgaa gatggaccaa tgggggatcg atgtcttaat
      781 cgcaggttct caaaaggggc tcatgctccc tccgggactt gcatttgtgg gaataagcga
      841 gaaggcctgg aagtgcgctg acaaggcaca gtgtccgaaa ttctatttta acttcaagaa
      901 ggaacgggaa tccctcgcca agaaccagac aaacttcacc tccccggtca ccttaatcat
      961 tggactaaat gagtgcctga agctgttaca gaaagagggg ctggagaacg cctttgaacg
     1021 ccataagagg cttgcccatg ctacgaggga ggcggtaagg gcaataggcc tgaaactttt
     1081 ttccagggaa tctcacagca attcagttac cgccattcaa tcacctgacg gtcttgacgg
     1141 ccaggagata tacaagaatc tcagggtcaa gtacggaatc accgctgcag gaggccaggg
     1201 ccaggcaaag ggaaagatat tcaggatcgc gcatctgggg tatgcggaca cttttgatat
     1261 tatcacggca gttgcagggg ttgagatggt attgaagtcc atgggacatc cgttgaagct
     1321 cgggacaggg gttgcgattg cagaagaact cttgatgaaa tgaccaggag gattgatgaa
     1381 agtactcata agcgataact tgtcccctcg gggaatcgaa gtgctgaaac agtcagggct
     1441 cgaagtggat gtgaagaccg ggatgaagcc tgaagaatta aaggcatgca ttggtgagta
     1501 tcatgggctt atcataagaa gcgcgacaaa ggttactgcg gagatcgtgg aagcagcagc
     1561 caacctgaag gtagtgggca gggccggctc agggcttgac aatgtggata aggcagctgc
     1621 cagcaagaag ggaatcgtgg tgatgaatac gccgggcggg aacaccatta caaccgcaga
     1681 acacaccata gccctcatgt ttgctcttgc acgacagata ccccaggcaa cggcctctat
     1741 gaaacaggga aaatgggaga agaagcgttt catgggggtt gagcttttca aaaagactat
     1801 cgggatcgtc ggcataggga atatcgggaa acacgttgca aggagagccc tgtcgctggg
     1861 gatgattgtc attggctatg acccgtatct cagtgacgag aacgcgaagg aaatggggat
     1921 agaaaaagtg gacttaacga cccttttcaa gcgctccgac tttatcacta ttcatacgcc
     1981 gatcacctcg gaaacgaaaa atctgatcag tacaaagacc atccagttga tgaaagacgg
     2041 ggtaaggatc ataaactgcg cacggggagg tattgtgaat gaggctgagc tttacgaggc
     2101 cctcaagaag gagaaggttg ccggtgctgc cctggatgtg tttgagaaag agcccccagg
     2161 ggaaaacccg cttttgaccc ttgacagtgt catatgcacc ccgcaccttg gagcagccac
     2221 agaagaggca caggagaatg ttgccgtggc catcgcagag caggtggtgg actgtcttgt
     2281 gaatggggtc atcagaaatg cggtgaattt tccctctgtg ccagcggagc aggtcctgag
     2341 gctgaaaccg tacctgaccc ttgcagagaa actgggaact tttgcctccc aggtgtttga
     2401 ggggggtgtg accgaaatca ccgtggaata caggggtgag gcatcccagc tcaataccgc
     2461 gccggtgaat attgcgctcc tgaagggctt cctaacaccc atccttgatg agacagtcaa
     2521 ttttgtgaat gcccctgtaa tcaccaagga gagggggatt gaggtcaagg agatgaagag
     2581 ccctgacgga ggcgattacc agagtatgat tattctcagg gtaaaggcgg accgtaagga
     2641 gcatcgtctt tcgggtaccc tcttggggag gactgatccc aggatcgtga ggatagacga
     2701 ttttgccgtg gagatcgtac cagaggggac catgctcttc atgtataaca acgataggcc
     2761 tggcgtgata gggaacatcg gctcgtatct cgggaaatgt aatatcaata ttgcgagaat
     2821 gcattttgga agagagcgcg aggggggaat ggccatatca gtggtgaata ttgactcgcc
     2881 gataagcgag tcgcaactgg aagatctcaa gaagatgcca aacatccttt cagtaaaggt
     2941 ggtggaactg tgagatgtga aatttacttg tcgaggggtt aggcaatggc atcagtagtg
     3001 atagtgggcg cgcaatgggg agatgagggc aaggggaaaa ttgtagatgt cctcacagaa
     3061 aaggcggatg tggttgccag ataccagggg ggccataatg cgggccatac cgtggtgatc
     3121 aagaacgaaa agttcgtgct ccatcttatc ccttccggca tattacataa agataaactc
     3181 tgcattatcg gaaatggggt agtgatcgag ccctcttctc tcatagatga gataaaagtg
     3241 ttgaaggaga agggcttgga gattggcaag aatctcctta ttagcaagac ggcccatctg
     3301 atcatgccct atcatgtcgc aatcgataat gaacatgaac gggcaaaggg ttcaagaaag
     3361 ataggaacca cgggaagggg aatagggcct gcctatgtgg ataaaatggg gcggaccggg
     3421 atacgggttg tggaccttta ctccccccaa gtctttaaag agaaactgga ggcaaacctt
     3481 cacgaggtga attacctcat cgagaaccgt tacaaggcaa agggatttag ccttgacgag
     3541 atttttgact tgtatatgga atacgcggag aagatttccc cccatgttgc ggacaccgac
     3601 attatcgtga atgacatggt gagggacggc aagaagatcc tttttgaagg ggcacagggt
     3661 acacttcttg atgtcgatca cgggacatac cccttcgtga cctcctcaag ttccattgca
     3721 ggcggcgcgt gcacaggact cggggtgggc cccactaaaa tcacaaaagt cctcggagta
     3781 gcaaaggcat ataccacaag agtggggggc gggccctttc ccacagagat ccacgatgct
     3841 gtcggggaag cactgaggca aaaaggcgga gaatatggtg ccactaccgg tagacccaga
     3901 agatgtggct ggctcgatct ggtggggctg cgccatgccc tcagggtgaa tgggttaaca
     3961 ggcctcgcga ttacaaaact ggatatccta gatgatctgg agaagatcaa agtttgtgtt
     4021 gcctacaagt acgggaaaca tctccatgag gatctcccca aggagatttc cctctttgag
     4081 gaggctgagc ctgtgtacga agagatggag gggtggaaag aaaaaaccgt cggcataacc
     4141 acattcaaag agcttcccgg aaacgcacaa cactatgtca ggaacatgga agaaaggctg
     4201 ggagtcaacg ttgatatgat ctctacaggg caacgcagag aagagcttat catgctcaac
     4261 gagcagttct gaatgcgcag gaaaagcaat agggttacga aacggctcga agttacgttc
     4321 tcttcaggtg gtctgcaatt cacaggaata tccagcgacc tttctgcaag cggacttttt
     4381 ataagaacgc aaaatggcct catgccggga accatagtcg aaattgagat atatctgcct
     4441 cagcacaaat taggccgtct caagggcata gtgaggagga cggtgaaaac ttccttatcc
     4501 atgataaaaa atggtatggg ggtggagttg atcgaaaggg accaaaatta ccttgatttt
     4561 gtgaagagca tccgcggtga agacaaagag caaagggagg agaccgacgc acagagcgga
     4621 gaagggaagg tccgtcagcc tgagggcaag gccaatgaaa cacctgattc cagaattatc
     4681 gtctgtgtga attgtgaaac aaaaaacagg atccccctgg gaagagttcc ccttggggcg
     4741 aagtgcggaa gatgtgggac gctcttatat ctaggggaca tcgactaacc tcttcccttt
     4801 tttctgcaat ttatccctcc acgtgtcccc tatgcaagaa ttcatctgac acccttctct
     4861 acgcaccaat ctgcacgcgc tgctgggaat cgatgacaag gtatttgggg ccgtcatgtc
     4921 gtgtgtgtgc cactcccctc gtctcagaat actcaacgct gtgcggggaa tgtctctccc
     4981 accctccacc cttttcaatg gtcttgaact tcggcctcta ttcaggttcc ctttctgcgg
     5041 cgattcatct cctgaaattc tcaggcctga aaaggcttgc aaaaccattg gggaaattat
     5101 tgacggaact tcccattccc tctatggacg gcattgtccc ggtaccagtg acgaaaagga
     5161 ctttaaggac ccggggattt aaccaggcgc tcttattatc cgtggttctt tcacgacacc
     5221 taagaatacc ggtgtatatg gacatgttat ataagaaaag ggatacaccc gcacagatag
     5281 gccttggagc taaggaacgt gcacgcaacg tcaagaacgc ctttgaagtt aggggtagat
     5341 tggataagcc tcgccttgtc ctgctggatg atgtcatgac cactggggcg acgg
//
LOCUS       DATKOU010000243         5287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1136065, whole genome shotgun sequence.
ACCESSION   DATKOU010000243 DATKOU010000000
VERSION     DATKOU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136065"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VMU21_11505"
     CDS             complement(<1..1020)
                     /locus_tag="VMU21_11505"
                     /inference="COORDINATES: protein motif:HMM:NF022580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HUO78188.1"
                     /translation="MLIKAIERFYDKPMITDLLYPFMFEDNAFLLIDGSLGMIWEIEG
                     INIDGKSDEEIQKASTTFSNFIKSLPVDVPMQIITATWRGLEKDILRVYKQGDLSNEY
                     INEYMTRKIEWHDHGKLHGFAHEGNIHFYPRTIKTYLTIKQKPLFANANLYDKKIMQE
                     TKDKLKRIEMIVDTSLSSGGIKHRRLSPDDLIALMYRMLNPERFLSDMPMAYKGGDLR
                     KFTVFNSPDADQHGWTFEGKKYSVISFANNPALPDDEGTLYTFPNILFREVNGISLYD
                     YTPMMLFTINFVLPSQDSLNRKLNFKRTMAFLHRFNILGDESIDKGIAREESKRLLTA
                     MYGGEK"
     gene            complement(1026..1523)
                     /locus_tag="VMU21_11510"
     CDS             complement(1026..1523)
                     /locus_tag="VMU21_11510"
                     /inference="COORDINATES: protein motif:HMM:NF021212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HUO78189.1"
                     /translation="MARAITTAIILVTVSSCATTNKQTDTPEQILSKAMGEGIRPAGR
                     ISTLPGADRSDLPYVPVVTPPEVARIWIYDHITPSRDLVVGHWIFIKLREEKWYIEEQ
                     WDDKSPGSAKRVPLPPSKPKTEKHTEHTHTGVSQRREAGVQTNTPGKAAAPVPGLTPP
                     PQAGR"
     gene            complement(1477..2667)
                     /locus_tag="VMU21_11515"
     CDS             complement(1477..2667)
                     /locus_tag="VMU21_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HUO78190.1"
                     /translation="MDVAKIAGLIAKHRKLLIFGVIVVIVIYALSTGSQKKAREEQAK
                     GKGNISTEERLKADIEGLKKKIDQMEREKGKAAAKGEEPEKGKKVGPTPLPQEKEKEK
                     IESLKELEKTLKPTSKLPLPNAGTGGPGTPSDIGGLPLKKQEPPRLLKIDISEITRPE
                     GKDGGSPPAQLDMFLPASSFASFTLTSSVYAPETGEQMPVSGVIDKAYIGPNGSSVPL
                     RGCFFLGKARGNTGEGIADIKVIKLACIWPDGRAFEGDIAGYITDTNGDFGLKGRVER
                     HAGTFFTTVGITSFLEGFAAGMSRAQEATTLGTSGLAVETVTNIAGSAAKYGLLKGAA
                     DFATAAKQFFAAQLSSLVPAVVVSAGAKGRVFITSGVNITGGMSALKDGKSYYDSYNL
                     SHSQ"
     gene            complement(2671..3324)
                     /locus_tag="VMU21_11520"
     CDS             complement(2671..3324)
                     /locus_tag="VMU21_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78191.1"
                     /translation="MIRSLLLAAILLLVTGGSLYAQKTVLYTGNPVVVKVAYLNPTEV
                     VFEGEDIASVILGFSPESISLQNTSRALFIQPLVENLTGDIFVVMKDSKSKILSVVST
                     VPDLRDKSVRIIDQVENVSGRIEKINRTGLTPAGLIKAMMLGEELDGVSVSPTKQVII
                     EIPIQLTADTVYDALFLKGYIVDMTDRQSFDIKSITMKGLIAGSMYKDKAYFVIQGE"
     gene            complement(3336..3935)
                     /locus_tag="VMU21_11525"
     CDS             complement(3336..3935)
                     /locus_tag="VMU21_11525"
                     /inference="COORDINATES: protein motif:HMM:NF017152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HUO78192.1"
                     /translation="MPLFVEYGELERFLIKVLTIIVTVESLVIMGLGFLAVKQRVVYV
                     NPSNVIGTTHVGYVPDDSAAYFGMTFIFFLGNVNEYSAKEQYKSAYLLMSPKLQSVMK
                     RTFEGEIAEIKNSDISVQTTPITYKVQGNGDEFTVIIEAIRKSYVYGQNAKKEKLQYT
                     ISCQKAHITKTNPFGLEVTSYENKVVAEGAAIAAGDQQQ"
     gene            complement(3987..4244)
                     /locus_tag="VMU21_11530"
     CDS             complement(3987..4244)
                     /locus_tag="VMU21_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78193.1"
                     /translation="MAWSPKYIDKPLRFLYDPEVWGVSFIAMGILMLFGLNQTLSAAM
                     MIPIGAVVQRTVDHHKPGFLPHIFYRAGMLDIFCPYGKYRF"
     gene            complement(4247..4570)
                     /locus_tag="VMU21_11535"
     CDS             complement(4247..4570)
                     /locus_tag="VMU21_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78194.1"
                     /translation="MKTLSVMIIAILLVGLAVGAVPSHAASSSGGGVDDSGGNSIVDA
                     LKSFAKGGWGKALFFCAMITGIICILFTRHRGFGLIALVFGILLGIYGGLGESLWTLF
                     TSWGA"
     gene            4818..>5287
                     /locus_tag="VMU21_11540"
     CDS             4818..>5287
                     /locus_tag="VMU21_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HUO78195.1"
                     /translation="MGKVHLGINKANPRETVQWETTEVVNGHWIVVGGSGSGKTHRIR
                     DVATQLQTQGMRMHVFDPHGDIDTDPAYTSAVEFSETSPYGINPLAINPSPAYGGVRK
                     RINSLIRTINKYSSKLDAREEAVMRYAMKELYEMHGFDYSNPGTWAQGSKRMPTL"
BASE COUNT         1208 a         1376 c         1269 g         1434 t
ORIGIN      
        1 tttctcgccg ccgtacatgg cggtgagcag cctttttgat tcctccctcg cgatcccttt
       61 gtctatggat tcatcaccga ggatattgaa gcgatgaaga aatgccatgg tccttttaaa
      121 gttcaacttc ctattcaggg aatcctgtga agggagaacg aagtttatcg tgaaaagcat
      181 catgggtgta tagtcataaa ggcttatgcc gttgacctcc cggaaaagta tatttgggaa
      241 ggtataaaga gtgccctcat cgtcgggaag ggcggggttg ttggcgaagc ttatcacgct
      301 gtattttttc ccttcaaatg tccagccgtg ctggtcggcg tccggcgaat tgaagaccgt
      361 gaatttcctg agatctcctc ctttgtaggc catcggcatg tcgctgagga acctttcggg
      421 gttcagcatt cgatacataa gagcaataag atcatccggg ctgaggcgcc tgtgcttaat
      481 gccgcccgac gacagggagg tatcgactat catctcgatg cgcttcaact tgtctttcgt
      541 ctcctgcatg attttcttgt catacaggtt ggcgtttgca aagagaggtt tctgttttat
      601 cgtaaggtag gtcttgatgg ttctcggata gaagtggata ttcccttcat gagcgaaacc
      661 gtgcaatttg ccgtggtcgt gccactctat cttccttgtc atatactcgt ttatgtactc
      721 attgctcagg tcaccttgct tatatacacg gagaatatcc ttttccagcc ccctccatgt
      781 tgcggtgatt atctgcatgg ggacgtccac gggcagagac ttaataaagt tcgagaaggt
      841 ggtggaggcc ttctgtattt cttcatcact tttgccgtca atgtttatgc cctcgatctc
      901 ccagatcatc cccaacgagc catcgatgag caagaatgcg ttgtcctcaa acataaacgg
      961 gtaaaggagg tccgttatca ttggtttgtc atagaatctc tcgatcgcct tgataagcat
     1021 tatcgttatc gtccagcctg gggcggtggg gtcaatccag gcaccggagc ggccgccttg
     1081 cccggtgtgt tcgtctgaac ccctgcctcc cggcgttggc ttactccggt atgagtatgt
     1141 tcggtatgtt tttccgtctt tggtttggac ggagggagag ggactctctt tgccgatccg
     1201 ggggatttat catcccattg ctcctcgatg taccatttct cttctctgag cttgatgaat
     1261 atccagtgtc ccacgacgag gtccctggaa ggcgtaatat ggtcgtatat ccagattctt
     1321 gcaacctcag gtggcgtgac cacaggcaca taagggaggt ctgacctgtc cgcccccggg
     1381 agcgtcgata tccttccggc tggacgaatg ccctcgccca tcgccttact gaggatctgc
     1441 tcgggcgtgt cggtctgttt gtttgtggtt gcgcagctac tgactgtgac taagattata
     1501 gctgtcgtaa tagctcttgc catccttgag cgcactcatg cctcctgtga tattgactcc
     1561 ggatgtaata aatacgcggc ccttggcgcc tgcagaaact accaccgcgg gaacaaggga
     1621 cgaaagctgc gcagcaaaaa actgttttgc agctgttgca aagtcggcag cgcctttcag
     1681 cagaccatat tttgcggctg agccggcgat attggttaca gtttcgaccg ccagccccga
     1741 agtcccaagc gttgtcgctt cctgggccct tgacataccg gcggcgaatc cctcaaggaa
     1801 agaggtgatc cccaccgtcg taaagaatgt acctgcatgc ctttccaccc tgcccttcag
     1861 tccgaaatcg ccgttagtgt ctgttatgta gcctgcgata tcaccttcga aggcgcggcc
     1921 gtcaggccat atacaagcaa gtttgatcac ctttatatct gcaatcccct cccctgtatt
     1981 gccgcgggcc ttgcccagga aaaaacatcc ccgcaggggg acagaagacc cgttcggacc
     2041 tatatatgcc ttatctatca ctcctgaaac gggcatctgc tctccggtct ccggagcata
     2101 gacggatgaa gtgagagtga aggatgcaaa ggagctggcc ggcagaaaca tatctaattg
     2161 ggcagggggg cttcctccgt cttttccttc ggggcgcgtg atttcagata tgtcaatctt
     2221 aagcagtctc ggcggctctt gtttcttgag aggcaatcct cctatatcag agggcgttcc
     2281 ggggccgccc gtgcctgcat taggcagagg caatttggac gtcggtttga gcgtcttttc
     2341 cagttcctta agggactcga ttttttcctt ttctttttcc tggggcaggg gagtgggtcc
     2401 gacctttttt cctttctcag gctcttcacc ctttgcggct gccttaccct tttctctctc
     2461 catctggtca atcttttttt tcaacccctc gatatcagcc ttcagcctct cttctgttga
     2521 aatgttcccc ttcccttttg cctgttcctc cctggctttt ttttgagacc cggttgagag
     2581 tgcatagatg acgattacca ctattacccc gaagatgagt aattttctgt gcttcgctat
     2641 taatcctgct attttagcca catccatgcg tcattcccct tgaatcacga aatatgcctt
     2701 gtccttgtac atcgaaccag ctatgagccc tttcatggtg attgacttaa tgtcaaagct
     2761 ctggcggtcg gtcatgtcta caatgtatcc cttcaggaat aaggcgtcat agacggtatc
     2821 agcggtcaac tgtatgggga tctctattat cacctgtttt gtagggctta ccgagacacc
     2881 gtccagttct tccccgagca tcattgcctt gatcaggccg gcgggtgtaa gtcctgtccg
     2941 atttattttc tctatcctcc ccgagacatt ttcgacctgg tcgataatcc tcactgattt
     3001 gtctcttaag tcggggacgg tagagactac gctcagtatt ttcgatttgc tgtctttcat
     3061 gacgacaaaa atgtctccgg taagattttc tacgaggggc tggataaaca gggctcttga
     3121 agtgttctgc agggatatgc tttcagggga aaaaccgagt attacggaag cgatatcctc
     3181 tccctcgaat acgacctctg taggattcag atacgcaact ttgacgacga cagggtttcc
     3241 ggtatacaga actgtcttct gtgcgtacag actgccgcct gtcacgagca gcaatattgc
     3301 agccaacagc aatgacctta tcatcgtatc agctctcact gctgctggtc tccggcagcg
     3361 attgccgcgc cttcagcaac aaccttgttt tcatatgagg ttacctccag gccgaaagga
     3421 ttcgtcttcg ttatgtgcgc cttttgacaa ctgatcgtgt actgcagctt ctcctttttt
     3481 gcgttttgtc cgtacacata ggattttctg atggcctcta tgatcacggt aaactcgtcc
     3541 ccgttgccct gaactttata ggtgataggc gtcgtctgaa cggaaatatc ggagtttttg
     3601 atttcagcta tttccccctc aaacgtcctc ttcattacgc tctgaagctt gggcgacatg
     3661 agaagatagg ccgatttgta ctgttccttg gctgaatatt cgttgacgtt tcccagaaag
     3721 aagatgaaag tcataccgaa gtaagcagca ctgtcgtcag ggacataacc cacatgtgtc
     3781 gtcccgatta cgttggaggg gttgacatag accactcgct gttttaccgc taaaaagccg
     3841 aggcccatga ttacgagcga ttctactgtc actatgatgg tcagtacctt gataagaaat
     3901 ctctccaact ctccgtattc aacaaaaaga ggcacctaaa gatatctcct tttcaaaaat
     3961 gtgcaccttg aagtttgctt gcggtttcag aaccggtatt tgccgtaagg acagaatatg
     4021 tccaacatac ccgcgcgata aaatatgtgc ggtaagaaac cgggcttgtg gtggtcaacc
     4081 gtccgctgaa ccaccgcacc aatggggatc atcatcgcgg ctgatagagt ctgattcaga
     4141 ccgaaaagca tgagaatccc cattgctatg aatgagacac cccacacctc agggtcataa
     4201 agaaacctca agggtttgtc gatatatttg ggcgaccacg ccattactaa gcaccccagc
     4261 tggtgaagag ggtccagaga gactctccca ggcctccgta tatcccaagg agaataccga
     4321 acacaagggc tatcagtccg aatcccctat gccttgtgaa gagaatgcaa ataatccccg
     4381 tgatcatggc gcagaaaaag agggccttcc cccatccgcc tttcgcaaag gacttaaggg
     4441 cgtcgacaat agagtttcct ccggaatcgt ccacacctcc gccgctactg ctggcggcat
     4501 gagagggtac agcacctacg gcaagaccca cgagcagaat tgcgatgatc attaccgaca
     4561 acgttttcat acaacactct cctttcaaac ctttcttata ttttcttatg agtactgctg
     4621 ctgtgcagcc atttcaagaa atatattatg ttggcccttc cggcagttcc ctggtccctg
     4681 ccgaaagatg ccgtcgaggg catgaaataa tatctatctg gaagtagatg aattccattc
     4741 ttcctccttt cccccccgaa ctgggagcat gcctccatgc tcccctcctt ttctgaacac
     4801 aaccttaata tcaatctgtg ggcaaggttc acttaggaat caacaaggca aacccgagag
     4861 agacagtcca atgggaaaca accgaggtcg tcaacggcca ctggatcgtt gtgggggggt
     4921 cgggttccgg gaaaacacat cgcatacgcg atgtcgcaac ccagcttcag acacagggca
     4981 tgcgcatgca tgtattcgac ccccacggcg atatagatac ggatcccgca tatacatctg
     5041 ctgttgagtt ctcggaaaca agcccttacg gtatcaatcc acttgcaata aacccctctc
     5101 ccgcatacgg aggcgtgaga aagaggatca actccttaat acgcaccatt aataaatact
     5161 cgtcgaaact tgatgcgagg gaagaggcgg tcatgagata tgcgatgaag gaactctatg
     5221 agatgcatgg attcgactac agtaatcccg gtacatgggc gcagggatca aagaggatgc
     5281 ccacgct
//
LOCUS       DATKOU010000244        14565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5734584, whole genome shotgun sequence.
ACCESSION   DATKOU010000244 DATKOU010000000
VERSION     DATKOU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5734584"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..443
                     /locus_tag="VMU21_11545"
     CDS             27..443
                     /locus_tag="VMU21_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78196.1"
                     /translation="MRIIKLSPKDRSMTNRDMVDYYFNEELPNREHGQFLFTKGRIKE
                     EGIAPGEMLVFSYNGDIVYLALSESERHANTGPEASEYPFYFCVDIKTIIEGKGNLKQ
                     LENEIKVNKNIVKTQGWPTIKDSLEIKRIWNKFKAK"
     gene            594..836
                     /locus_tag="VMU21_11550"
     CDS             594..836
                     /locus_tag="VMU21_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HUO78197.1"
                     /translation="MGKTKIAITLDEQYIDQLDTFVSKHIFQNRSQAIQQAVKEKLAR
                     MKRTRLATECSKLDPTFEKAMADEGLTEDLSRWPEY"
     gene            821..1159
                     /locus_tag="VMU21_11555"
     CDS             821..1159
                     /locus_tag="VMU21_11555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HUO78198.1"
                     /translation="MARILRGDIRWADLNLVRGSEQAWLRPVLILSQDIFNERSGTVI
                     AVALTSQPQKAGFPLTLEITTAHLPKKSWVKISQIRTLSVERIGKMMGRVSPEEIERV
                     VEGLNEIIGA"
     gene            1228..1587
                     /locus_tag="VMU21_11560"
     CDS             1228..1587
                     /locus_tag="VMU21_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78199.1"
                     /translation="MKREHWLIIIAAAIGILIWVVVSTVASKSEAWDSPLYFVVGMPL
                     LCVVAGVLALIEPDHPWKWAVVPLAAQAAWMFMTQGFGNLAPLGVLFFGVLAIPLFIA
                     ARIGAFFGKRRAKPTLP"
     gene            1694..1891
                     /locus_tag="VMU21_11565"
     CDS             1694..1891
                     /locus_tag="VMU21_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HUO78200.1"
                     /translation="MKTKLKMIYWKGEKFWVGKLLNHPEIMTQGKTLAELEENMKEAY
                     MLMTMDDVPPKHKVKELSLSV"
     gene            2153..2641
                     /locus_tag="VMU21_11570"
     CDS             2153..2641
                     /locus_tag="VMU21_11570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO78201.1"
                     /translation="MFIEVLTEEQIEIVESLAEEIWTEHYVPIIGKAQVDYMLDRFQS
                     KKSISDQIMSGFLYFLIKEDNQFIGYIGVQPKGDELFLSKIYIKSSERGKGLGGKAIQ
                     FIERLAKEKGLGKIVLTVNKNNRDAIKAYEKLGFKNLGSLIQEIGGGFIMDDYKMEKT
                     LL"
     gene            complement(2636..3994)
                     /locus_tag="VMU21_11575"
     CDS             complement(2636..3994)
                     /locus_tag="VMU21_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HUO78202.1"
                     /translation="MNKLRDALPHIHASTDPEDLICYGFDACGLEELPSAVLWPENTE
                     DIIAIMRYAQAEGIPVVPRGAGTGMSGGAVPLRNSVVLSMEKMNRLLELDKDNLTVLV
                     EPGIINGRLQREVEDQGLFYPPDPSSMNFCTIGGNVAENAGGPRALKYGVTRDYVMEI
                     EAVLPSGDLITAGVKTQKGVVGYDLARLLVGSEGTLAVATKIRLKLLPLPEDVVTLLC
                     LFNDLEASGKAVSMIIASRIIPRTLEFMDREAIRAVEKFQPLGISDTIAALLLIEIDG
                     YPDAIRKEAEKVAGICMKLGADVSMAKDYEAKNRLWECRRRASQALYHISPRKISEDI
                     VVPRSRIPEMLVSLQRLSEETDISIVNFGHAGDGNIHVNLMVDRTNEEEYRRAESLAR
                     EIFRVTLDLGGTISGEHGVGITKSPFIGMEIKKRELELMRGIKKLFDPKNILNPGKIF
                     PS"
     gene            complement(4099..7632)
                     /gene="smc"
                     /locus_tag="VMU21_11580"
     CDS             complement(4099..7632)
                     /gene="smc"
                     /locus_tag="VMU21_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HUO78203.1"
                     /translation="MRIEKIELIGFKSFAEKTTFLLHPGITCIVGPNGAGKSNIVDSF
                     RWVLGEQSAKSLRGEKMEEVIFNGSSTKKPRGMSEVNLILSFDSPEQGNGDSQSLTSV
                     ARRLYRSGESEYLINKRQCRLRDIKDLFLDTGLEIKSYSILEQGRIGEILNSKPLERR
                     FLIEEVAGVMKYKVRKTEALSKLESSRLNIQRINDIVVEVKRQINSLDRQAKKAERYK
                     KLSSEMRSIELKIAKKDYSSLRESLEKITADSVILKEKDASVRAAITTIENAVETKRI
                     TLLDKEKFLDSLVSELQVLEREMAETERGIAVSTTEQNNLTEYTTRLIQQESDLVQKI
                     EGVRDRIKELDTTERNLEQESESLRQELSEKNEGIRSIERDFTQKEELLESKRKEIFR
                     SAEEISHLRNEVGKLLTTLDNLEKNYLSTLKEAETAKEQLASAEALLGDTEGTLIAKN
                     SELLLLNDTKAKIIADIDEYRSKRDQARSSLSTLREDLASSSSRLHSLQEILHEDLSR
                     ETLAEHLRIMASVAEVIDVDEAYEKAIENALSEEINGFIIQSPEEVSAAASVLKEKGL
                     RRTAFIPLEAHSGSRGGAYELPAHMHESVVGRADELVRFHPGYDSFSSVIKGLLDRVV
                     VVRDLSAALSLFTEASMNNKDLAPTIVTMDGETVEPSGAIIVGEGKGILKRKREIREL
                     DSLIEQKKSEMERLALALTEVESALQARQTSLKEIEATVIHAEKEISLLRLTVETQTE
                     EKEKLSRKLAYLTMEGAELLKEKESVKGALAEQETEVGLIDSVKGEAEQLLSRLQDAI
                     SSDRERYEEERSSVTELRLSLNSYKERIESLGKEVESSREMLSDLSRNHETVIKEKSD
                     IDARIMQCTEVISKNNEGLRELVLKADRLKTTIAEQRELIRTESEELVHSEQGAKTHR
                     LALDSLTASIGEADVSLAEHRLKIENISDNIRRNYDIEISSFETEPVLPEDGERLSEL
                     RTKIQELGSVNLGTLEEYEELKTRYEFLTKQQEDLNKSIAELEEAITKINSTTRRKLR
                     EAFEQLNTKFSEVFTLLFGGGKAGLVMTDENNILESGIEIIAQPPGKKLQNITLLSGG
                     EKALTALSLLFASFLIKPSPLCILDEVDAALDESNIERFAKMLRELSSGIQFIVVTHN
                     RVTMEAADYIYGITMEEPGASKVISMQLVDS"
     gene            7893..9077
                     /gene="dsrA"
                     /locus_tag="VMU21_11585"
     CDS             7893..9077
                     /gene="dsrA"
                     /locus_tag="VMU21_11585"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HUO78204.1"
                     /translation="MAKKHQTPLLDELEKGPFPSFVTEIKKAATKSDMAADELGQLEK
                     SYRDQRGYWKHGGIVGVRGYGSGVIGRYSSLPEEFPNVANFHTVRINSISGFFYTSKF
                     LKEMCDIWDKYGSGLTNLHGSTGDLVLLGTTTENLEPIFAEFSSRGFDLGGSGSALRT
                     PSCCVGPARCEWSNINTLDITYSITQEWQDEMHRPAFPYKFKFKTAGCAVDCIASIAR
                     ADCSIIGTWKGKIAIDQAEVANYAKKGTNISEEIVNRCPSRCMSYDGKELKIADEDCI
                     RCMHCIAKMTKALKPTGERGATILIGSKAPFVIGATLSWVIVPFIKMEPPYTEFKDLI
                     RKMWEFWDEHGKNRERIGELIIRKGMREFLEYIGVNPVPQQVKEPRRDPFFFWTEQDL
                     QK"
     gene            9111..10181
                     /gene="dsrB"
                     /locus_tag="VMU21_11590"
     CDS             9111..10181
                     /gene="dsrB"
                     /locus_tag="VMU21_11590"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HUO78205.1"
                     /translation="MSAPQRKTDIGPPHYKDFLPPVIQKNYGDWKYHEVINHGVMVHV
                     ANNGDKLWSVRVASPRLLSTDTIRDFAGMAEKYCGGYLRFTTRNNVELLVSDEKNLEP
                     LQKELKSKKYMIGGIGSGISNIVHTQGWVHCHSACTDASGLVKVLMDEFADYFTTKHT
                     PNKVRLAVACCVNMCGAVHCSDIAVVAIHRKIPVVDHDKVKNVCEIPSTIASCPTGAI
                     SPDPAKKSVKVNPDKCMYCGNCFTVCPPMEIHDPEYDGVAIVVGGKVGNLRSNPKFSK
                     LAVPFIKNNPPRWPEVTAAVRKIFDAYMAGAKKHERVGEWIERIGWEKFFKVTGLPFT
                     FQHIDDFTIARETFRTAATFKW"
     gene            10262..10477
                     /locus_tag="VMU21_11595"
     CDS             10262..10477
                     /locus_tag="VMU21_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78206.1"
                     /translation="MEKEEVKQKIYALVEKSVGKKKLKSSDIQKTVSAEAGISRDEVK
                     DALKDLVDEGKLIYTYFGGSFVEIPPK"
     gene            10931..11791
                     /locus_tag="VMU21_11600"
     CDS             10931..11791
                     /locus_tag="VMU21_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUO78207.1"
                     /translation="MKTRILLVDDDETMVKSSKHALESHGYEVDGEIDAERALEKLKT
                     VSYDLMITELVMLKLDGIRLIRNAKIINPSIGVVVLTGYPSPEAIRHALQVGAIDFLA
                     KPFFPEVLGDVVAKALSYRKTAQVYAAQDEFDMKKINAILQENKNRPERLIPVLQDIQ
                     EITGYLPPSLQKLVSRELNVPISKVSGVVTFYFMFTMTPKGKHKIRICTGTACHVKGA
                     EQNYRRVKGYLGMQDDETMTKDKFFSVETVRCVGACSLAPVTDFDGAIEGLLTPDAIT
                     KIIDQTRRGA"
     gene            11791..13671
                     /locus_tag="VMU21_11605"
     CDS             11791..13671
                     /locus_tag="VMU21_11605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HUO78208.1"
                     /translation="MLIQSESDLERIKEDFELRHHVRINLLLCGGTGCRATKSAAVRD
                     SFEEELKKRGITDTAITLTGCKGFCANGPTLVVDPGGYFYQKVKAEDAPLIVEEHILN
                     NRPIEKLMYLDPATKARVPLMRDIPFFKKQEIRVLRNLGRIDPFSINHYIARDGYQAA
                     VKAILHMTPEQIVSEVRLSGIRGRGGAGFLTGLKWELCAKSPGSLKYIICNGDEGDPG
                     AFMDRGVMESDPHAVIEGMIIAARAIGSRKGYIYVRAEYPLAIETLEAALRQARAYGL
                     LGANLFGTDFSIDIEIYKGAGAFVCGEETALISSIEGDRGNPVPKPPFPVHKGLWERP
                     TVINNVETLANIPQIILRGADWFKSVGTERSTGTKVFALTGDIQNIGLIEIPMGIPLK
                     QIIEDIGGGVPKGRKLKAVQLGGPSGGCIPHDMLDTPVTYEDIVKTGAIVGSGGMVVM
                     SDRSCMVDVAKFFIGFTKDESCGKCVACREGTKVMLTILEDITEGRATLDDLNTLEDL
                     ANDVRKSSLCGLGQTAPNPVLSTIRYFRHEYEEHILNKRCSTGACRELTTFHIDPEKC
                     TGCTACALKCAVTAISGEKKKPHHIDQSRCITCRMCYETCKFGAIHIGPGAHFAGKEV
                     PA"
     gene            13668..>14565
                     /locus_tag="VMU21_11610"
     CDS             13668..>14565
                     /locus_tag="VMU21_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUO78209.1"
                     /translation="MIELTIDGKALSVPKGTSILKAALDHGIFIPHLCYDPRLERHGG
                     CRMCLVELEGQPRLFASCSTPVDNGMKVHTHTPQVNKLRQTMLELMLASHPLDCPTCD
                     KAGECVLQDLAYKYGSTKGRFKSLRNNAPQHENPLVDTNTNRCVLCGKCVRICWEQQG
                     VGALNFQHRGATTIVSTAFNETLNCEFCGQCMDVCPVGALTPRPSKHRSRVWFMETRH
                     AICPFCSVGCTLDLDIHDQTIIRSRGRYPHGVNRGNLCAKGRYGYDFIYTDDRLTTPL
                     IRREGVLQRASWEEALDYIAESL"
BASE COUNT         3694 a         3549 c         3681 g         3641 t
ORIGIN      
        1 aaaaataatg ttcggagaaa taaaacatga ggataataaa attaagcccg aaagatcgca
       61 gcatgacaaa tcgcgatatg gtcgattact attttaatga agaacttccc aatagagaac
      121 acgggcaatt cctttttacc aaaggacgaa ttaaagaaga aggcattgct cctggtgaga
      181 tgctagtttt ttcgtataat ggcgatatag tgtatcttgc cttatcagaa tcagagagac
      241 atgccaatac cggtcccgag gcaagcgaat atccgttcta tttctgtgtg gatatcaaaa
      301 caattattga aggcaaaggc aatttgaaac agcttgaaaa tgaaataaag gtaaacaaaa
      361 atatcgtaaa aactcagggc tggccaacca tcaaagattc acttgaaata aaaaggatat
      421 ggaataaatt caaggcaaaa tgatctccca acaatttgct ccagacgatg caggattaac
      481 cgtttttctt tttagtcctg cgtctgtgga ggggtcggct gatgcaggcg ttatgaagca
      541 aaaattgaca aaatcaaagg tatgatacaa agtaataccg gaggtgtttt catatgggca
      601 aaacaaaaat tgcgattacg ctggatgaac aatacattga tcagttagat acttttgtga
      661 gtaaacatat ttttcagaat cgtagccagg ctattcagca ggcagtaaag gaaaaactgg
      721 cacggatgaa acgcaccaga ttagcaacgg aatgctcgaa gcttgacccg acctttgaaa
      781 aggcaatggc ggatgaagga ttgactgagg atttgagccg gtggcccgaa tactaagggg
      841 cgacattaga tgggctgatc taaatctggt gcggggcagt gaacaggcct ggttacggcc
      901 tgtcctcatt ttaagtcagg atatttttaa tgaacgatca gggacggtga tagctgtagc
      961 tctcaccagt cagccccaga aagcgggatt ccctcttacc cttgaaataa caactgcaca
     1021 tttgcccaag aagtcgtggg taaaaataag tcagattcgg acgctttctg ttgagcgaat
     1081 tggaaaaatg atgggaaggg tttcacctga agaaatagaa cgggttgttg aaggactgaa
     1141 cgagataatt ggggcataac gtctaaggcg tgtgggtgac gctagccgcc gcacagttca
     1201 gccgttgacg caaatctgaa ctcgcacatg aaacgagaac attggctaat tattatagca
     1261 gcagcaatcg gaatcctgat ttgggttgtg gtctccactg tcgcatcaaa gtctgaagcg
     1321 tgggactcgc cattgtattt tgttgtcggc atgcccctgc tttgtgtggt agccggagtt
     1381 cttgctttga ttgaaccgga ccatccctgg aaatgggctg tggtcccttt ggcggcacag
     1441 gcagcctgga tgttcatgac acaggggttt gggaatctcg cgccgttagg agtcttgttc
     1501 tttggagtgc tcgcgattcc gcttttcatc gcagcgcgga ttggagcttt tttcggcaaa
     1561 agaagggcta aacctacatt gccctaacgt gcgtttcgcg ccgcctttgc taaaaaagat
     1621 agactatagt gccgccacca ttataatctt ttgtgctatc ataatttaag aatggcactg
     1681 agaggtatga agaatgaaaa ctaaattaaa gatgatctat tggaaaggtg agaaattttg
     1741 ggtcggaaag ttactcaatc atcctgaaat aatgactcaa ggcaaaacat tggcagaact
     1801 tgaggaaaat atgaaggaag cttatatgct catgaccatg gatgatgttc ctccaaaaca
     1861 taaagtcaaa gaactctctc tttcggtttg aaaagaaaag aattaatcaa gaaaatcacc
     1921 tcctccggct gtgtgttgct gaggcatggt caacggcacg atctctatct caatcccaaa
     1981 accggcaaga agcagccggt accgcgtcac agagaaatag acgaaaacct ttcacggcac
     2041 ataatcaaag aattgacatg acatcgccta caatcagacg ctgaataggt tcgtcttgat
     2101 cttcattcct gctgcagtgt ccgcgctgat aaggctgtag tcatctgaaa ccatgtttat
     2161 tgaagttctg actgaagaac aaatagaaat tgtcgagtct ctggcagaag agatctggac
     2221 cgaacattac gtccccatta tcggcaaagc gcaggtggat tatatgctcg acaggttcca
     2281 gtcaaaaaag tccatttcag atcagatcat gagcggtttt ctctatttcc tcatcaagga
     2341 agacaatcag tttatcggat atataggtgt ccagccgaaa ggtgatgaac tattcctgag
     2401 taaaatatac ataaaatctt ccgaacgtgg caagggtctt gggggaaaag ccattcaatt
     2461 cattgaaaga cttgcgaaag aaaaaggact aggaaaaatt gtactcaccg tgaacaaaaa
     2521 caacagagac gcgatcaagg catatgaaaa gctcggtttt aagaatcttg gttccctcat
     2581 ccaggaaatc ggcggcggtt ttatcatgga cgactataaa atggaaaaaa ccctcttatg
     2641 acgggaaaat cttgccaggg ttcaggatgt ttttcgggtc aaagagcttc ttaatccccc
     2701 tcatcagctc aagctccctc ttctttatct ccatcccgat aaagggagac tttgtgatgc
     2761 ctaccccgtg ctctccggaa atggttccgc caagatcaag ggtcacacgg aagatctccc
     2821 tggcaaggga ttcggcccgc cggtattcct cttcattggt cctgtcaacc ataaggttca
     2881 catggatgtt gccgtcccct gcatggccaa aattcactat ggaaatgtcg gtctcctcag
     2941 agagcctctg caaactgacg agcatctcag gaatcctgct tctggggacc acgatatcct
     3001 cacttatttt cctggggctg atgtggtaaa gggcctggga agcccttctt ctgcattccc
     3061 ataacctgtt tttcgcctcg taatcttttg ccatactcac atccgcaccc aatttcatgc
     3121 agatacctgc caccttctcc gcctccttcc tgatcgcatc agggtaccca tcgatctcta
     3181 tgagaaggag tgcggctatg gtgtcagaaa tcccaagggg ctggaatttt tcgacagcac
     3241 gtatggcttc cctgtccata aactccagag ttctgggaat gatcctcgac gcaataatca
     3301 ttgatacggc cttccccgag gcctcgagat cattaaacag acaaagaagg gtgaccacat
     3361 cctcgggcag gggaaggagc ttcagcctta ttttagttgc caccgccagg gtcccttccg
     3421 agcctacgag caatctcgca agatcatatc ccaccacgcc cttttgggtc ttgacgcctg
     3481 cggtgatgag gtcgccgctg ggcagcaccg cctcgatctc catcacataa tcccttgtga
     3541 cgccgtattt caatgccctt ggccctcccg cgttttccgc tacattaccc ccaatggtgc
     3601 agaaattcat acttgaaggg tcaggcgggt agaaaagacc ttggtcttcc acctccctct
     3661 gcaatctccc gttaatgatc ccgggctcca cgagcacggt gaggttgtct ttgtccagtt
     3721 caaggaggcg gttcatcttt tccatgctca ggaccacaga gttcctgaga gggaccgcac
     3781 ctccgctcat gcctgtgccc gcgccccgtg ggaccacagg gattccctcg gcttgggcgt
     3841 atctcatgat tgcgatgatg tcctctgtat tctccggcca taggactgca gaaggaagtt
     3901 cctccagtcc gcaggcgtca aacccatagc atatgaggtc ctcggggtct gtggatgcgt
     3961 gtatgtgggg caatgcgtct cttaatttat tcatcttgtg ttcattccaa aaagattcca
     4021 gggtgtgggg caatgaatga gtggagtgcg tacatcgtgg tgtcttgcag agtggcccct
     4081 gctctgccca ctctcgtttt atgaatctac aagctgcatg gagattacct ttgaagcgcc
     4141 aggctcttcc atggttattc cgtaaatgta gtccgcagcc tccatggtta ccctgttgtg
     4201 ggtcaccaca atgaactgta tgcctgaaga gagttctctg agcatcttcg cgaaccgctc
     4261 gatattggat tcatccaggg cagcatccac ctcgtcgaga atgcagagag gtgaaggctt
     4321 tatgagaaaa ctcgcaaaga ggagcgacaa ggcagtgagc gccttctctc ctccggaaag
     4381 gagggttatg ttctggagct ttttgcccgg cggctgcgca ataatctcta tgcctgactc
     4441 gaggatattg ttctcatctg tcatcacaag gcctgccttc cctccgccaa acagcaaggt
     4501 gaacacttca gagaacttgg tgttgagttg ttcaaaggcc tccctgagtt ttcttctggt
     4561 cgtactgttt atttttgtga tggcctcttc cagttctgca atggatttat tgagatcttc
     4621 ctgctgcttg gtgaggaatt catagcgcgt cttaagctct tcatattcct caagcgtgcc
     4681 gagattcaca gagccaagtt cctggatctt tgtccttagc tcggaaaggc gttctccatc
     4741 ctcaggaagt acgggttcgg tctcaaaaga ggaaatctca atgtcatagt tcctccgaat
     4801 gttgtctgag atattttcaa ttttcagcct gtgttctgcg agggaaacat cagcctcgcc
     4861 tatggaagcg gtcaatgaat ctaatgcaag gcgatgggtc ttggccccct gctctgaatg
     4921 aacgagttcc tctgactcgg tccgaatgag ttctctctgt tctgcaatcg ttgtcttgag
     4981 acgatcggcc ttgaggacaa gctctcttaa cccctcgttg tttttgctga tcacctccgt
     5041 gcactgcatt attctcgcgt caatatccga tttttctttt atcaccgtct catggttcct
     5101 tgagaggtca gagagcatct cccgggagct ctccacttcc tttcccaggg attcaatccg
     5161 ttccttatag gagttcaggg ataacctcag ttctgtgacg gatgagcgtt cttcctcata
     5221 gcgctccctg tctgaggata tggcatcctg cagacgggag aggagctgtt cagcctcccc
     5281 cttcaccgaa tctatcagac caacctcggt ctcttgctca gcgagggccc cctttacgga
     5341 ttctttttcc ttcagaagtt cagctccctc catagtgagg tatgcaagct ttctgctgag
     5401 tttttctttc tcctcagtct gggtttccac cgtcaatctt aaaagcgata tctctttttc
     5461 cgcatgaatt accgttgcct cgatctcttt cagcgacgtc tgtctcgcct gaagtgccga
     5521 ctccacctct gtgagggcga gcgcaagacg ttccatttct gacttcttct gctcaatgag
     5581 ggagtcaagc tctcttatct ccctctttct cttcaagata ccctttccct cgcccacgat
     5641 gatcgctccc gagggctcca cggtctcacc atccatggtc acaattgttg gtgccaggtc
     5701 cttattattc atgctcgctt cagtaaagag tgaaagtgcg gcagagaggt ctcttaccac
     5761 taccaccctg tcgagaagac ccttgattac cgaagagaat gaatcatagc caggatgaaa
     5821 ccttacaagt tcgtcggccc ttcccacgac ggattcgtgc atgtgagcag gtagttcata
     5881 cgcgccaccc ctactccctg agtgggcctc cagagggatg aaggctgtcc tccgtaaccc
     5941 cttctccttt aagactgagg ctgcagcaga gacctcttca ggggattgaa tgataaaacc
     6001 gtttatttct tctgagaggg cattctctat tgccttttcg taagcctcgt caacatcgat
     6061 gacctcagcg acggatgcca tgatgcgaag gtgctcggcg agcgtctctc ttgaaaggtc
     6121 ctcatgcagg atctcctgga gagaatggag acgggaggaa gaagaggcaa ggtcttcacg
     6181 cagggtggag aggctgcttc tggcctgatc acgttttgaa cggtactcat caatatcagc
     6241 gatgatcttt gcctttgtat cgttgaggag gaggagctcg ctgtttttgg caatcagcgt
     6301 tccctcggta tctcccagga gggcctctgc agatgctagc tgctccttcg cggtctcagc
     6361 ctcttttaac gtggagaggt agttcttctc gagattgtcc agggtagtta acagtttgcc
     6421 gacctcgttc ctgagatggc ttatttcttc tgccgacctg aagatttcct tcctctttga
     6481 ttccagtagc tcttcttttt gggtgaaatc cctctctatg cttctgatgc cttcattctt
     6541 ttcgctgagt tcctgcctga gggattcact ttcctgctca aggttccttt ctgtggtgtc
     6601 cagttccttt atccggtcac ggaccccctc aattttctgg acaagatctg attcctgctg
     6661 gatgagcctc gtcgtatact ctgtgagatt gttctgttct gtggtggata cggcgatacc
     6721 cctctcggtt tcggccatct ccctttccaa cacctgaagc tctgaaacga gggagtccag
     6781 gaatttttcc ttatcaagaa gggtgatccg ctttgtctca accgcgttct ctattgtggt
     6841 aatggctgcc ctcaccgatg catctttttc ctttaagatt acggaatcgg cggtgatttt
     6901 ctccagagac tctctcaggg aggagtaatc tttcttcgcg attttcagtt cgatagacct
     6961 catttcagag gagagtttct tgtagcgttc ggccttcttc gcctgcctgt caagggagtt
     7021 tatctgccgt tttacctcta cgacaatgtc atttattctt tggatattaa gccgcgagga
     7081 ttcgagtttt gagagcgctt cggtctttct caccttgtat ttcatgacgc ctgcgacctc
     7141 ttcgatcaga aaccttctct ccaggggctt tgagttaagg atctctccta ttcttccctg
     7201 ttccaggata gaataactct ttatctccag ccccgtatcc aggaaaaggt ccttgatatc
     7261 cctcaatctg cactgcctct tgttgatcag gtactcgctt tccccggacc ggtagagtct
     7321 ccgcgctacg gatgtgaggc tctgggaatc accgttaccc tgttcgggag aatcaaagga
     7381 caggataagg ttcacctcgg acatgccgcg cggctttttg gtggaggagc cgttaaatat
     7441 gacctcctcc atcttttctc cccgaagact cttcgcactc tgttcgccaa ggacccagcg
     7501 gaatgaatcg acaatattgc tcttccctgc gccattggga cccacgatgc aggttatccc
     7561 cggatggaga aggaacgtgg ttttttcagc aaaggactta aaccctatga gctcaatttt
     7621 ttctatgcgc attgcaattc ggcggcgaaa tatttttgag ggttgtagta taacctctgc
     7681 cggatggtta tgacaagcgt ataatttctg ccccttaaga agtgcagata gttctgaacc
     7741 aggataaata agcaaatatg atttatggat taattttttt aatttgaccg tcaagttaag
     7801 catatattac gcttagacga tttcatttcc agtcctattc tttaaaggta aagtatcaag
     7861 ggtcggaaga atcaaaataa aaggaggatt ttatggcaaa gaagcatcag actccattgt
     7921 tggatgagct ggaaaaaggc ccattcccga gctttgtaac agagatcaag aaagcagcta
     7981 ctaagagcga tatggctgct gatgagctcg gacagctcga aaagtcgtac agagaccagc
     8041 gtggttactg gaagcatggc ggtatcgtgg gtgttcgcgg ttatggttcc ggtgttatcg
     8101 ggagatattc ttcccttccc gaagagttcc ctaatgtggc aaatttccat acagtaagaa
     8161 tcaattccat ttcaggtttt ttctacacat caaagttcct caaagagatg tgtgacatct
     8221 gggataagta tgggagcggt ctcaccaacc tgcacgggtc gacaggtgac ctggttctgc
     8281 tcggaaccac gactgaaaac cttgaaccga tctttgccga attctcctcc cgcggctttg
     8341 acctcggtgg ttctggctct gcattgagga cgccgagctg ctgcgtcgga cctgcccggt
     8401 gtgaatggtc gaacataaac acccttgaca tcacttacag cataacgcag gaatggcagg
     8461 acgagatgca ccgtccggcg ttcccttata aattcaagtt taagacagcg ggatgtgcgg
     8521 ttgactgtat cgcctccatc gcccgtgcgg actgctccat catcggcacc tggaagggca
     8581 agatcgccat tgaccaggcg gaagtcgcca actacgcaaa gaagggcacg aatatttcag
     8641 aagagatcgt gaacagatgc ccgagcaggt gtatgagcta tgacggcaag gaactgaaga
     8701 ttgcagacga agactgcata cgctgcatgc actgtattgc gaagatgaca aaggcgttga
     8761 agccgaccgg ggaaagaggt gcgaccatcc ttatcggcag caaggccccc tttgttatcg
     8821 gagcaaccct ttcctgggtc atagtgccct tcataaagat ggaacccccg tatacggaat
     8881 ttaaagatct gataaggaaa atgtgggaat tctgggatga acacggaaag aacagggaga
     8941 ggatcggcga actcatcatc agaaagggaa tgagggagtt cctcgaatat atcggagtga
     9001 atccagttcc tcagcaggta aaggaaccga gaagagaccc attcttcttc tggacagaac
     9061 aggatttaca aaagtaaaca aacacattta gagataaaag gaggaagatt atgtcagcac
     9121 cacagagaaa aacagatatc gggccacctc attacaagga tttcctgccg cctgtgatcc
     9181 agaaaaatta cggcgactgg aaataccatg aggtcataaa tcacggtgtt atggtccatg
     9241 ttgccaacaa cggggacaaa ctctggtcgg tcagggtcgc atcaccacgg cttctcagca
     9301 ctgatactat cagggatttt gccggaatgg cggaaaagta ttgcggcggt taccttagat
     9361 ttaccacgag aaacaatgtt gagctcctcg tttccgacga aaagaacctc gagccgctcc
     9421 agaaggaatt gaagagcaag aagtacatga ttggaggtat cggttcggga atcagcaata
     9481 tcgtccatac ccaggggtgg gtgcactgcc attcggcatg taccgatgca tcggggttgg
     9541 tgaaggtcct catggacgaa ttcgccgatt attttacgac caagcatacc cccaacaagg
     9601 tgagacttgc ggtcgcctgc tgtgtgaata tgtgcggcgc ggtgcactgt tcggacatcg
     9661 cggtagttgc gattcacaga aaaattccgg tggtagacca tgataaggtc aagaatgtct
     9721 gcgagatccc gtccacgata gcatcctgtc cgacaggcgc aatcagccct gaccctgcaa
     9781 agaagagcgt caaggtgaac ccggacaagt gcatgtactg cgggaactgc tttaccgtat
     9841 gtccgcccat ggagatccat gaccctgaat atgacggtgt tgcgatcgtt gtgggaggta
     9901 aggtcggaaa cctcaggtct aacccgaagt tctcaaaact ggctgttccc tttatcaaaa
     9961 acaatcctcc taggtggccg gaagtcactg cagcagtcag aaagatcttt gacgcttata
    10021 tggcaggggc gaagaaacac gagagggtcg gtgaatggat cgagaggatc ggctgggaaa
    10081 agttctttaa ggtgaccgga ctgcccttta cctttcagca cattgatgac tttacgattg
    10141 cgagggagac cttcaggaca gcggcaacat ttaaatggta atttaagtat aatgtgtata
    10201 gcataaaggg gggatcgcaa gatccctcca attattaaca ttctaatgat gaggtggcag
    10261 aatggagaaa gaagaagtta agcagaagat atacgccttg gtagaaaaat ccgtggggaa
    10321 aaagaaactg aaatcttctg atattcagaa aaccgtttct gcggaagccg gcatttcgag
    10381 agatgaagta aaagacgcat taaaagacct cgtcgacgaa ggaaaactca tttataccta
    10441 tttcggcggt agtttcgtag agatcccgcc aaaatagaag gctcagctcc tgtgatttat
    10501 tgaaaggcag tccattcgat ggatgaactg ccttttttta tattctgttc tgtcagagac
    10561 agtgattaca gtttacttgt tattgatccc gactaatgcg aaagcagagg tatgcctgac
    10621 tcttcatgtg ccggttattg tgtaaacgct gtgagcttcc tgatgctctt ttcgtaacaa
    10681 cttcattgaa caagtcccga catcctttct ccgtcaaaga agaattatcc ggttgtctaa
    10741 acttatgctt tcaagttgac aagcctgcta ttgtccgatt taaggtaaag agagggtatc
    10801 ggggaacaca caataagaaa tcggaaggaa tgcgtctgta tatgagtatg gcaagtatgc
    10861 tcaacaacat aaccaaacca acaatagaac gagctaaaag aggagggcct gtaatgaatg
    10921 ggaggacact atgaaaacac ggattctgct tgttgacgat gacgaaacaa tggtgaaaag
    10981 cagcaagcat gcccttgaga gccatggata cgaagtggac ggcgagatag atgccgaaag
    11041 ggctttggag aagctgaaaa cggtttcgta cgatctcatg atcaccgaac tggtgatgct
    11101 gaaacttgac ggtattcggc tcattcgcaa tgccaagatt atcaacccct ccattggtgt
    11161 ggtagtactc accggctatc cctctccgga agcgatacgg catgcgcttc aggtgggggc
    11221 aattgacttt ctcgccaagc cctttttccc cgaggtcctg ggtgatgtgg tggcaaaggc
    11281 gctttcctac agaaagactg cccaggtgta tgcagctcag gacgaattcg atatgaagaa
    11341 aattaatgcg attctgcagg agaacaagaa ccggcctgag cggctcatcc cggtgctcca
    11401 ggatatccag gagattaccg gctacctgcc cccttcgctg caaaaactgg tgagcagaga
    11461 actgaatgtc ccgattagca aggtctccgg tgtggtcacc ttctacttca tgtttaccat
    11521 gacgcccaaa ggcaagcaca agataagaat ctgtaccggc actgcctgtc acgtgaaggg
    11581 agctgaacag aactaccgga gggtgaaggg ctacctcggc atgcaggatg acgagacaat
    11641 gacaaaggac aagttcttct ccgttgagac ggtgcgatgc gtgggtgcgt gcagtcttgc
    11701 ccctgtgacg gatttcgacg gagcgatcga aggacttctc actccggatg cgataacaaa
    11761 aataatcgat cagaccagga gaggtgcata atgctgatac agagcgaatc ggacctggag
    11821 aggatcaagg aagatttcga gctgcggcat catgtccgta taaatctcct cttgtgcggt
    11881 ggaaccggct gccgggccac aaagagcgct gcagtaagag attccttcga agaggagctg
    11941 aagaaacgag gcatcacgga tacagccata acactcaccg gatgcaaggg gttctgcgca
    12001 aatggtccca cactcgtggt cgatcccgga ggatactttt atcagaaggt caaagccgaa
    12061 gatgccccgc tgattgtgga agagcatata ctgaataatc ggccgataga gaaactcatg
    12121 taccttgacc ccgccacaaa agcgagagtc ccgctgatga gggatatccc gttttttaag
    12181 aaacaggaga tacgcgtcct gcgcaatctc ggtcgtatcg atcctttcag catcaaccac
    12241 tatatcgccc gtgacggcta ccaggcggct gtcaaagcca ttctccatat gacgccggaa
    12301 cagatcgtct ccgaagtgag attgtcaggg attcgcggcc gcggcggtgc gggttttctt
    12361 accggcctga aatgggaact gtgcgcaaag agccctggca gtctcaagta tattatttgt
    12421 aatggtgatg aaggagatcc aggggcgttt atggacaggg gggtgatgga atcagacccc
    12481 catgcggtca tcgagggaat gatcatcgct gcaagggcca taggctcccg caaagggtac
    12541 atctatgtgc gtgccgagta ccccctcgca atagagacac tggaggctgc gctgaggcag
    12601 gcgagagcat acggtctgct cggtgcaaac cttttcggaa ccgatttttc catagacatc
    12661 gagatatata agggcgcagg ggcctttgtg tgcggtgaag agacggcgct gatctcctct
    12721 atcgagggcg atcgtggcaa cccggttccg aagcccccct ttcccgtgca taagggccta
    12781 tgggaaaggc ctaccgttat caacaatgtc gaaaccctgg ctaacatacc ccagataatc
    12841 ctgagagggg cggactggtt caagagtgtc ggaacagaaa gatccaccgg cacgaaggtc
    12901 ttcgctctca cgggggacat ccagaacatc gggctcatag agattccgat gggaatcccg
    12961 ctgaaacaga tcatcgagga cataggcggt ggtgtgccga agggcaggaa actgaaggcc
    13021 gtgcagctcg gcggcccttc gggcggctgc atcccccatg atatgcttga tacaccggtc
    13081 acgtacgaag acatcgtaaa gacgggggcg attgtgggct caggcggcat ggtggtgatg
    13141 agtgaccgga gttgcatggt ggatgtggcg aagttcttca ttgggttcac caaagacgaa
    13201 tcgtgcggga agtgtgttgc ctgcagggaa gggacaaagg tgatgctgac cattcttgaa
    13261 gatatcaccg aggggcgtgc cacgctcgat gacctcaaca cattggagga tcttgcgaac
    13321 gatgtgcgca agtcctccct ttgcggcctg ggtcagacag cccccaaccc ggtactcagc
    13381 acgatccgct atttccggca tgaatatgag gagcacattc tcaataaacg ctgttctacc
    13441 ggtgcatgcc gtgagcttac caccttccat attgacccgg aaaagtgcac gggatgtacc
    13501 gcgtgcgcat tgaagtgcgc tgtgaccgca atctctggag aaaagaagaa accccaccat
    13561 atcgatcaat ccagatgcat cacctgccgc atgtgttacg aaacctgcaa gttcggagcc
    13621 atccatatcg gcccgggcgc ccattttgcg ggtaaggagg tgccggcatg atagagttga
    13681 ccatagacgg taaggctctg tctgttccga aagggacgtc gatacttaag gctgccctag
    13741 accatggcat tttcatcccc catttgtgct atgacccaag gctcgagcgt catggcggat
    13801 gcaggatgtg tctcgtcgaa ctcgagggtc agccccggct ctttgcctcg tgctcgaccc
    13861 ctgttgacaa cgggatgaaa gtacacaccc acacccccca ggtgaataaa cttcgtcaga
    13921 ccatgcttga actgatgctc gccagccatc ccctggactg cccgacatgc gataaagcag
    13981 gcgaatgtgt tctccaggat ctcgcatata agtacgggtc gacaaagggc cgcttcaaat
    14041 cgcttcgtaa caacgcgccg cagcacgaaa accctcttgt ggacaccaac accaaccggt
    14101 gcgtcctttg cggaaagtgc gtgaggatct gctgggagca gcagggcgtg ggcgccctca
    14161 acttccagca tcggggcgcc actaccatag tctccacggc atttaacgag accctcaatt
    14221 gcgagttctg cggtcagtgc atggacgtct gtcccgtagg cgccctaact cccagaccct
    14281 ccaaacaccg ctcgcgcgtc tggtttatgg agacccgcca cgctatttgc cccttctgca
    14341 gtgtgggctg cacccttgac ctggacatcc acgaccagac catcatccgc tcccgtggca
    14401 ggtatcctca tggggtgaac aggggaaacc tctgtgcaaa gggccgctat ggctatgact
    14461 ttatttacac cgatgaccgg ctcaccacac ctctcattcg gcgcgaggga gtgcttcagc
    14521 gagcctcctg ggaagaagca ctcgactata tcgcagagtc tctca
//
LOCUS       DATKOU010000245         5577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4206111, whole genome shotgun sequence.
ACCESSION   DATKOU010000245 DATKOU010000000
VERSION     DATKOU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4206111"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..874
                     /locus_tag="VMU21_11615"
     CDS             86..874
                     /locus_tag="VMU21_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUO78210.1"
                     /translation="MILGKKLFVIMPAYNAEMTLRQTYEELPHEYVDEVILVDDASKD
                     ETAKVAAELGIRTIIHSENKGYGGNQKTCYREALGHNADIVIMVHPDYQYSPRLVAAM
                     ASMIASGHYDVVLGSRILGGTALKGGMPLYKYFANRILTLLENLALGVKLSEYHTGFR
                     AFTREVLETLPLEENSDDFVFDSEMLVQTVWFGFRIGELSCPTRYFKEASSINFRRSV
                     KYGFGVLGSILKYLTGKWGLREYTIFSNSGKKLLTDNGNTRTHL"
     gene            complement(906..1088)
                     /locus_tag="VMU21_11620"
     CDS             complement(906..1088)
                     /locus_tag="VMU21_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78211.1"
                     /translation="MFQITYKPDCYLRHTILIGALRSISSTDGEMTGLGHFTIRKDLI
                     ALSSSADKFILKCRER"
     gene            1142..1870
                     /locus_tag="VMU21_11625"
     CDS             1142..1870
                     /locus_tag="VMU21_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUO78212.1"
                     /translation="MVYKLHSVHPMERALIVNDDQLMSYAISKALQPYHPEIKTVSNG
                     DDAIAEISSCFYPLCFLDVSVPGLHGSDMVRKIRELSPDTKVVVFTPEERPVNGAKDF
                     IGEKIDENACFCLQKPFEISELKAVVQQALNKKEQEPFSLNKKDQKFFSADKRIPRRP
                     LKKCVSYTVIVLEQGKPVSLTLKGDIIDISDKGVGMRTHYPLEPGHYLMFDNETELKS
                     GVVKWISPPDDSYMYRVGVEFLEL"
     gene            complement(1899..3290)
                     /locus_tag="VMU21_11630"
     CDS             complement(1899..3290)
                     /locus_tag="VMU21_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO78213.1"
                     /translation="MKKGKVFLVDDDELIVSMLSRALRGEGYEVNSETVTFTAVVDKI
                     AAWLPDVVLLDLKLPSITGLDILEKIKTNQIDTQVVMLTSDDHVETAIKCMKLGAADY
                     LTKPFNIDEVKIVVNNIVEKRSLKSEVSYLRRFYSEAFRKDIIGESPAMAELKQKAEK
                     VARSNVSTVLITGESGTGKELVARYIHSIMYDESGKGFAPFIAINCTALPETLLESEL
                     FGYEKGAFTDAKAEKKGIFELAHGGTILLDEIGDMKLSLQGKLLRVLEERTIRRIGGQ
                     KEIPIEVTVIATTNRNLSEAVDRGEFRMDLFYRLNIFALSLPPLRERKEDIPLLAQHY
                     LTHFTSQYKNKVIKGFSPRVEQVMYSYRWPGNVREMKNVIERIVVLETSELIMPEHLP
                     REMLGQAVLPPPHASARFVLPEAGISLDELERDLITQALERAKSNKTVAAKLLNITYD
                     SLRYQIKKFGLEE"
     gene            complement(3287..5125)
                     /locus_tag="VMU21_11635"
     CDS             complement(3287..5125)
                     /locus_tag="VMU21_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUO78214.1"
                     /translation="MKKKIIIIFGITTLAFLVSGGYIISTIQNTTSELDTLIKLHQVE
                     ILREHLLIRIKRVQSDLNLRTTRFARGVDVMVQDVAGMEEAVAVCFGCHHSPAVAGRL
                     EGLRNHIEQYKDSLSRVLTIRANAKRLDAEEEHTFKMGEELISEVDTMIALTSTKLTG
                     KTEAALGEIAKTKVMLFILVAIGPFLALALAVLIIRGFTRPVHALLDATRKIKGGDLD
                     YRVRALKDEFGEVAESFNEMAGSLREHLIKVEESETRYRALFERAGDAIFVIDAEGEK
                     KFRITAANPAAAKIHGYTVDELLRLKITDLDAPEAMGQTPLLAQRILRGEWVTEQTYH
                     VKKDGTVFPLEVSAGLMDLGNHKYILAFDRDITERRQAEEALQRTEQLKVVGELAAGL
                     AHEIKNPLSGIKASMEVLSEEAALSEEDRGVLIRVVSEIKRIELLLKGLLSFTRPPKP
                     HFSTVNLNALLDTSLLFSLKNTSYSPDTSGPITVVKKFDEHLPETMADPMQLQQVFMN
                     ILINAADAMREGGTLTVQTGFEASEDAIEILISDTGRGMDREEMENIFLPFFTTKPKG
                     SGLGLPISKRLIEQHGGSLRVESAVGKGSTFRISLPVRRVAGVQFV"
     gene            complement(5125..>5577)
                     /locus_tag="VMU21_11640"
     CDS             complement(5125..>5577)
                     /locus_tag="VMU21_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUO78215.1"
                     /translation="AGYLLPLEYFHHHGVKNYRTYLGETYFAGTHEDAIHDVLEKKAD
                     IGAAKNTVFERMAKDDPRINKELLVLVKSPEVPENGLAVRRDLDETLKQDIKAALLTM
                     DKEAAGQAVLRTFGAQRFIETTDNDYAPVIEYAREIGLNLSTYDYLNN"
BASE COUNT         1279 a         1475 c         1364 g         1459 t
ORIGIN      
        1 catgagcgaa tgaaaagatc tctattttca aaaagtggtc aagcaaggca ttgcacagtc
       61 tatgaacagg taaggggtaa ccttaatgat tctcggtaaa aagctttttg taataatgcc
      121 cgcatataat gcggaaatga cgttgcgtca gacctacgaa gagctcccgc acgagtatgt
      181 ggacgaagtg attcttgttg acgatgcatc caaggatgaa acggctaaag ttgcggcgga
      241 actgggtatt cgtacgatca ttcattctga aaacaaggga tacggcggca accagaagac
      301 ctgctatcgt gaagcactcg gccataacgc tgacattgtc atcatggtgc atccagatta
      361 ccaatattcc cctaggcttg tggctgccat ggcatcgatg atcgcctcgg ggcattatga
      421 tgtcgtcctc ggttcgagaa tactcggggg tacggccctg aaaggaggta tgcctttata
      481 taagtatttt gcaaacagaa ttcttacact ccttgaaaac ctggcccttg gcgtcaaact
      541 ttccgaatac catacggggt tccgggcatt cacacgggag gtgctggaaa cgttaccgct
      601 ggaagagaat tctgatgatt ttgtctttga cagcgagatg ctcgtccaga ctgtgtggtt
      661 cggcttcagg attggtgaac tgagttgtcc cacaagatac tttaaagagg cgtcctctat
      721 aaatttccga aggagcgtaa aatacgggtt cggtgtgctt ggcagcatac taaagtatct
      781 gacaggcaag tggggcttga gggaatatac gatattcagc aacagcggaa aaaaacttct
      841 cacggataat gggaacacga gaactcacct atagtctcgc cctgctgttt ccttcctgac
      901 ttttctcatc tctcccggca cttcagaatg aatttgtcgg ctgatgaaga cagggcgatg
      961 aggtcttttc tgatggtgaa atgaccaagt ccggtcattt caccatcagt cgaggagata
     1021 ctccttaacg cgccaatcag gatggtatgg cgaaggtagc aatccggttt ataggtaatc
     1081 tgaaacaact ttcgtctgct cacaatgcgt actacaattt tgtaaagggg tccttcctga
     1141 ggtggtatat aaattgcact ctgtccaccc gatggagcgg gcacttattg tgaatgatga
     1201 tcagttgatg agctatgcca tttcaaaggc ccttcagcca tatcatcccg agatcaagac
     1261 tgtttccaat ggagatgatg ccattgccga gatcagttcc tgtttttatc cgttatgttt
     1321 cctcgatgtc tctgtcccgg gactgcacgg gtctgacatg gtcagaaaga tcagagagct
     1381 ctccccggat accaaggtgg tcgttttcac tcctgaagaa cgtcctgtca acggcgcaaa
     1441 agacttcatc ggagagaaga ttgatgagaa tgcatgcttt tgccttcaga aaccctttga
     1501 gatttccgag ttgaaagccg tcgtgcagca ggccctaaac aagaaagagc aggaaccttt
     1561 ctccctgaat aagaaagacc agaaattttt ctccgctgac aagaggatcc caagaagacc
     1621 cctgaagaaa tgcgtgagct acacggtcat tgtccttgaa caaggaaagc cggtgagtct
     1681 gaccctcaaa ggcgatatta tcgatatttc cgacaaaggg gtcggcatga ggacacacta
     1741 tccccttgag ccgggacatt atcttatgtt cgacaatgag acagaactaa agtctggtgt
     1801 agtgaagtgg atctccccgc cggacgactc ctacatgtac cgggtggggg ttgagttcct
     1861 ggagttgtaa cgaaccgcag cacgaaaatt ctccctgact attcttccag cccgaatttc
     1921 tttatctggt atctgagcga gtcataggtt atgttcagca atttcgccgc aacggtcttg
     1981 ttgctcttcg cccgctccag ggcctgggta ataaggtctc tttccagttc atcgagggag
     2041 atccctgcct cgggaagaac aaacctggct gaggcatggg gaggcggaag aaccgcttgt
     2101 cccagcatct ccctcggaag atgttcaggc attatcaatt cagaggtctc gagcacgacg
     2161 atccgctcta tgacattctt catctctctc acgttgcccg gccagcgata ggaatacatc
     2221 acctgctcca ccctggggga aaaccccttg atcaccttgt ttttatactg agaggtgaag
     2281 tgggtgagat aatgctgcgc gaggagaggg atgtcctctt tcctctccct gaggggcgga
     2341 agggagagag cgaagatatt cagccggtag aaaaggtcca tcctgaattc tcccctgtcc
     2401 accgcctcgg agaggttcct gttggtggtg gcgatgaccg ttacctctat cggtatttcc
     2461 ttctgccctc ctattctccg tatggtcctc tcctccagca cccgcaagag cttgccctgg
     2521 aggctgagct tcatgtcacc gatctcgtca aggagtatcg tccccccatg tgcaagttcg
     2581 aatattccct ttttttccgc ctttgcatcg gtgaaggctc ccttctcgta cccaaaaagc
     2641 tcgctttcaa gaagggtctc gggcaatgcg gtgcaattga tcgcgatgaa gggagcaaac
     2701 cccttcccgg attcgtcata catgatactg tggatgtaac gcgctacgag ttcttttccg
     2761 gtcccgcttt cacccgtaat aaggaccgtg gacacgttgg agcgcgctac cttttccgcc
     2821 ttttgcttca attccgccat ggcaggagat tcccctatga tgtccttgcg aaacgcctca
     2881 gaataaaatc tcctgaggta actcacctcg ctcttgaggc tcctcttttc gacaatgtta
     2941 ttgacgacga tcttcacctc gtcgatattg aaaggcttgg tgagatagtc cgctgcgccg
     3001 agcttcatac actttatggc ggtttccaca tggtcatcgg aagtgagcat caccacctgg
     3061 gtatcgatct ggtttgtttt tatcttctcg aggatgtcca gccctgtgat ggacggaagc
     3121 ttcaggtcca gcaataccac atcaggcaac cacgcggcga ttttgtccac aacggcagtg
     3181 aaggtgacgg tctctgagtt gacctcgtat ccctcgcctc gtagggccct tgagagcatc
     3241 gagacaataa gttcatcgtc atctacaaga aataccttcc ctttcttcat acgaattgga
     3301 ccccggccac gcgtctcacg ggaagtgaga tcctgaaggt gctccccttc ccgacagcac
     3361 tctccacgcg gaggctcccc ccgtgctgtt cgatgagacg tttcgaaata ggcagcccga
     3421 gaccgctgcc tttgggtttt gtggtaaaaa acggcaggaa gatattttcc atctcctccc
     3481 tgtccattcc cctgccggta tcagaaatca ggatctcgat cgcgtcttcc gaggcctcga
     3541 acccggtctg taccgtcaga gtcccccctt ccctcatcgc atctgccgcg ttaataagga
     3601 tattcatgaa tacctgctgc agttgcatgg gatcagccat ggtttcaggg agatgttcat
     3661 cgaatttttt cacgacggtg atcggccccg atgtatccgg tgaataggat gtatttttca
     3721 aagagaagag aagagaggtg tcgagcaggg cattcaggtt caccgtgctg aaatggggct
     3781 taggcggcct cgtaaaactg aggaggccct tcagcaggag ttctatcctc ttgatttcgc
     3841 tcaccaccct tatcagcact cccctgtctt cttcagacag cgcggcctct tcggagagca
     3901 cctccatgga cgccttgatg cccgagaggg gatttttgat ttcgtgggcc agccctgctg
     3961 ccagttctcc caccaccttc agttgctctg tcctctgaag ggcctcttcc gcctgcctgc
     4021 gctcggtgat gtcacggtca aatgcgagga tatatttatg atttcccaga tccatcaacc
     4081 cggcactcac ctcaaggggg aagacagtgc cgtctttttt aacatggtat gtctgctcag
     4141 tgacccattc acccctgagt atgcgctgag cgagcaaagg ggtctgcccc atcgcctcag
     4201 gggcatcaag gtcggttatt tttagcctga gcagctcatc aacagtgtac ccatggatct
     4261 ttgccgctgc cggattggct gccgtaatgc gaaatttctt ttcaccctct gcatcgatga
     4321 caaaaatggc atcacctgcc cgctcaaaaa gcgcccgata ccgtgtctcg ctctcctcta
     4381 ctttaatgag atgttcccta agtgaacctg ccatctcatt aaaagactcc gccacctccc
     4441 caaattcatc cttcagggct cttacccggt agtccagatc tccgcctttg atcttccgcg
     4501 tggcatcaag gagggcatgc accggcctgg tgaatccccg gataatgagc accgccaatg
     4561 ctaaggcgag aaagggacct atagcgacga ggataaaaag catcactttt gtttttgcga
     4621 tctcgccaag ggccgcttct gtcttgccgg tgagttttgt gctcgtcagg gcgatcatgg
     4681 tgtcaacctc tgaaatcagc tcctctccca tcttaaatgt atgctcctct tccgcgtcca
     4741 atctcttcgc gttcgcccgt atggtgagaa ccctgctcaa tgaatcctta tactgttcaa
     4801 tatgatttct cagtccctca agcctccccg ccaccgccgg agaatgatgg cagccaaagc
     4861 acacagccac cgcttcttcc attccggcca catcctgaac catgacatct acgccccttg
     4921 caaagcgtgt ggttctgagg ttgaggtcgg actggacccg tttgatccgt atcaagaggt
     4981 gctccctcag gatttcgacc tggtggagct tgatgagggt gtccagctct gatgtggtat
     5041 tttggatcgt gctgatgata tacccgcctg atacgagaaa cgccagggtg gtgatgccaa
     5101 agatgatgat gatcttcttt ttcatcagtt attcaggtaa tcataggtgg agagattgag
     5161 accaatctct cttgcatact ctattaccgg cgcgtaatcg ttatcagtgg tttcgataaa
     5221 cctctgtgct ccgaacgttc tcaaaacagc ctggccggcc gcttccttgt ccatggttaa
     5281 gagcgcggcc tttatgtctt gtttgagggt ttcatccagg tcccttctca ccgcaaggcc
     5341 attctcgggc acctcggggg atttcacgag taccaacagc tccttgttga ttctggggtc
     5401 gtctttcgcc atcctctcaa ataccgtgtt ttttgcagcg ccgatatcag ctttcttctc
     5461 gagcacatca tggattgcgt cttcatgggt ccccgcaaaa tacgtctcgc ctaaatacgt
     5521 cctgtaattc ttcacgccat gatggtgaaa atactccagg ggcagaagat accctgc
//
LOCUS       DATKOU010000246        15045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5360400, whole genome shotgun sequence.
ACCESSION   DATKOU010000246 DATKOU010000000
VERSION     DATKOU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 15045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15045
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5360400"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..792
                     /locus_tag="VMU21_11645"
     CDS             439..792
                     /locus_tag="VMU21_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78216.1"
                     /translation="MKLWEKKGMQGWIKRYEKNMTTEKNTIIKWKFQRGITEEEKDAA
                     IKAGLAKISNYAGVKHSSASEIATKTGDIFNTSKFLGHRSGIQNTMKHTERRDIEKLR
                     KLQDEIVIPPTLISN"
     gene            complement(865..1215)
                     /locus_tag="VMU21_11650"
     CDS             complement(865..1215)
                     /locus_tag="VMU21_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUO78217.1"
                     /translation="MATTKDVLERHLKCFGEGALTGILSDYAMDAVFFTPNGPLKGPD
                     AIRPLFEHLLAEFGKPGATFTMKLRSVEGDYAYILWTAQTADNVYELATDTFVVQDGK
                     IVAQSFTGKVTPKG"
     gene            complement(1676..1945)
                     /locus_tag="VMU21_11655"
     CDS             complement(1676..1945)
                     /locus_tag="VMU21_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78218.1"
                     /translation="MQDALVQQAYTAVVEYFVKTGRAPHYTELAGILKVRPEEARQVQ
                     HKAAESSIACWFIKDTDYVESWAPFSNVPTHHRVTIKGEQKWYGQ"
     gene            complement(2341..3180)
                     /locus_tag="VMU21_11660"
     CDS             complement(2341..3180)
                     /locus_tag="VMU21_11660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUO78219.1"
                     /translation="MEIPKRTLGNTGIEVTVMGLGGEGILRTYGYEKEAAQLINTAID
                     LGVNYFESARAYSGSELYYGKALKERRREIFLASKSHARGRRGALNHLHETLKQMMTD
                     HLDLWQVHDMRTEEDIKEVFGPDGALQAFADAKKRGLVRFIGVTGHHDPSIIKRCIQD
                     FDFDTVLIPVNPAEPVYKSFIDEVIPIAQGKGMGIVGMKAYLRGLAQRIPGYGTLEPF
                     FRFALSHPITTAVIGCDSIRQLEENVGFARAFNAMGREEMQGTIDAVSPYARQLMYYK
                     PSN"
     gene            complement(3298..3987)
                     /locus_tag="VMU21_11665"
     CDS             complement(3298..3987)
                     /locus_tag="VMU21_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUO78220.1"
                     /translation="MNTWTRRYERFAVEDMALQAKTLFATEIELLDVSMTGACIIAKK
                     SLKTGDQHLLKLHNKEMAFTLQGVVVWVTFGGSVKNVREEAVPVYRAGVMFKNISSDK
                     LVKLKDFMRTFGIPDGEKVLDEYPPTALRFRINATEGAVLYYYKTSPVKKISLSGMLM
                     EGYNGIKLEWRAPMALFLPNGNAPLKFRGRVASCIEIPGRRSKHYDIGVEFLDMPEDD
                     RSRLSEFLHLL"
     gene            4277..5260
                     /locus_tag="VMU21_11670"
     CDS             4277..5260
                     /locus_tag="VMU21_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78221.1"
                     /translation="MKKLSGLFIITMLTLSLAALFNGCGGGSSPSTSRGGGTDATLTA
                     SNAPQVADVVRQAVKLVAPATLGQVNATSVSPEKRAPLMSIFEKVAPVIGKHATNGKQ
                     SSDILPSENCSGGGSIEVTNISVPDLSGNVKADVNVNGCTIGTETMNGVLKVTVPVGA
                     LTDILHTEKFTIEVTSFTYSDSTTTSLSLTDNFTILAMDFTYDGNSLTGGSVTLGGTV
                     SGTIHGNPVNISCDSLGSHFNVNSSGVAVSVSGRMSTSCLGGWVTLSTNLPVFVPADA
                     NCPTGGEIVVSAGGSSVTMHVEANSKIDIFFNGALVQKYDDCSQVIGLCTG"
     gene            complement(5293..6207)
                     /locus_tag="VMU21_11675"
     CDS             complement(5293..6207)
                     /locus_tag="VMU21_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUO78222.1"
                     /translation="MIYYGSCSWTEKSLIRSREFYPKGVNTAEKRLRYYSSTFDVVEV
                     DSSYYAIPSVEMVSRWAERTPPGFLFHIKAYSLLTGHSADLKTTPPEVRDMLSTDSLK
                     KDRVALRDKKFLQMAFRLFKEALSPLAETHKAGVVVFQYPPYFVYKPQNLEYILLCKD
                     MMEDFQVGVEFRHGSWLTPDRRDGVFSFLRENEITYITADEPQYGNLSTVPFIPQVTT
                     DTAYFRLHGRNKETWLRKGIETSLRYDYLYSDEELKSFVPSLVKIDSTAAKTFVMFNN
                     CHLGYSMKNALRLREMMKEKEGVESEGG"
     gene            6361..7416
                     /gene="pdhA"
                     /locus_tag="VMU21_11680"
     CDS             6361..7416
                     /gene="pdhA"
                     /locus_tag="VMU21_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HUO78223.1"
                     /translation="MPEKIIESFSVKRLDILDEQGEVDTSLMPSLTDDQIKRMYEVML
                     LARTFDQRALSLQREGRIGTYASILGQEASQIGSALALEKTDWVFPSFRENGVYITLG
                     HPPHMILQYWSGDERGLRTTEDKNIFPVCIPVASHLPHAVGAAMAAKYRKDRTAVAAY
                     FGDGGTSKGDFHEALNFAGVFRLPVVFICQNNQWAISVPREKQTSSKTIAQKAIAYGF
                     QGIQVDGNDIFAVYRATKEALDRAKGGEGPTLIECFTYRVSDHTTADDASRYRKKEEV
                     EAWKAKDPLLRLRLYMEKKGLWTAEYQKEVELRARAEVDEAEKKAESIAPPDPRDMFT
                     YTYAALTQRLLREIKDF"
     gene            7418..8380
                     /locus_tag="VMU21_11685"
     CDS             7418..8380
                     /locus_tag="VMU21_11685"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HUO78224.1"
                     /translation="MPKLNMVQAINLALREEMQRDKDVLILGEDVGRDGGVFRVTEGL
                     LEQFGQERVVDTPLAESVIVGAAIGMASYGLRPVAEIQFLGFIYPAMDQISNHSSRLR
                     SRSRGRYSCPLVIRAPYGAGIKAPEHHSESCEALFCHMPGIKVVMPSSPYYAKGLLIS
                     SIRDPDPVVFLESTRLYRLIKEEVPEGEYTVPLEKARLVQEGKDVTVISWGFMLHRVL
                     QAVEGFDAEVIDLMTLNPLDEETIYGSVKKTGRVVIVNEAPRTGSFGSELSASIAEDV
                     MLFLKSPVMRVSAYDTVLPLPRLEDHYMPSVEKIRKAIADVMKY"
     gene            8465..8908
                     /locus_tag="VMU21_11690"
     CDS             8465..8908
                     /locus_tag="VMU21_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO78225.1"
                     /translation="MPATGPDELFEAGLEALARGDSLSAITFFERAVQMEDRPAFSCY
                     LGLCIAKERGQFQLAVTLCEKAKAREPHNPLHYLNLGRVYLIAGKKDDAIRTFREGLG
                     YEQSQPIIDELENLGTRKPSIISSLKRSNPVNKYLGIILKRLGLR"
     gene            complement(8916..9830)
                     /locus_tag="VMU21_11695"
     CDS             complement(8916..9830)
                     /locus_tag="VMU21_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUO78226.1"
                     /translation="MKRMRMHGKAFRLLFLCFIAALLFFSSLSQGYALDHQKSFLWKV
                     QSKSSTVYILGSLHALRREMYPLPGKIEDAFSKSDVLVVEANINEMPLEGIMRMLEGA
                     LYMGNEGLESHLSKETYELAKARLNRFGMPVEFFHKSKPWFLALMITALEMQRLGFDP
                     EYGIDSYFLKKAVGVKSVMELESVTYQMNLLSTLSEGDQESFLLYTLRDLDILSGEMD
                     TLTKAWSTGDTEALESVVSKGVQDDPRMLPIYEKLIYERNNTMTSRIEGFLKTGGRYF
                     VVVGAGHLVGKKGIIELLKKKGYSVEQW"
     gene            complement(9891..10316)
                     /locus_tag="VMU21_11700"
     CDS             complement(9891..10316)
                     /locus_tag="VMU21_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78227.1"
                     /translation="MLEMYSGAVEIKTVIMRGALKNKPKESFIPQERHETIRKEIMDI
                     LEGNSFSAKDISGEVGISVKEVFEHLEHIQRSVSRGDYHLAMTPAECKKCGFVFRKRE
                     KLKKPGKCPVCRGESIQEPLFSLRRTEILHNNNNNGQER"
     gene            10607..10969
                     /locus_tag="VMU21_11705"
     CDS             10607..10969
                     /locus_tag="VMU21_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78228.1"
                     /translation="MFDRKNIKSVLAVIVAALLVTAFAYQASAYSVGDNTSYTYTGKV
                     AEVDNVYKYVTVQTGSNTTFSFKLDDYATVMKCDKIGSLADLKPGDLVTVYYFQENLG
                     GRHIASEVDLTAYGMKSC"
     gene            complement(11232..12143)
                     /locus_tag="VMU21_11710"
     CDS             complement(11232..12143)
                     /locus_tag="VMU21_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78229.1"
                     /translation="MEQGKTRKSYALLSWLVTIMLLCSVTVIGILYKDKITPLEGELK
                     KIQIVSSLRIHLLEAIEAEKNAVLAITDEASKTYADEARSASNKVENARRDLAAIIQQ
                     TGTSKEQETVAEFNSCWIEFRKLDEEILELATQNSNLKAQKLSSTQFYREVAAFEKSL
                     RTLIQHDMRDNRPGFITVHSYESITAILQLFALHKAHIEEPDDQEMDRIERKMKDYLD
                     TAQKALHALRSIPELRGSAELVNAESAYKRIVEINAEVVRLSRLNTNIKSSALSLGKK
                     RLISAKCQEILGALQETIESHRFKATR"
     gene            complement(12239..14092)
                     /locus_tag="VMU21_11715"
     CDS             complement(12239..14092)
                     /locus_tag="VMU21_11715"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HUO78230.1"
                     /translation="MMKKLAISATLVIFLFNQLVLASDFRFSPRPNKTHLIKWKHWER
                     QSLDDAQREGKPILLSLSAVWCHWCHVMDETTYSAIDIIDYINSNFIPVRVDVNMRPD
                     IDNLYNQGGWPSTVVLTPEGEIVQGGNFISQESMIAWLSDALEMLKEDKKGFKEKSET
                     IKKKKRKGTGRQMDGTAPDVSDIARITNMLESSYDKRHGGFGLSQKFPNPDAIDFLLS
                     GYLNKRNPELMTIISTTLENMSEGEIYDRVEGGFFRYSTESDWSAPHYEKMLDLNAAL
                     IRNYASAYMVFGNNKYKKVLNKTITYVEKYLYDKKAGAFYGSQDAGEEYYRRTERAGF
                     KPPYVDSTIYADSNAQMITGLIAAYGATSEKRLIRMAVRTADFLLKNLYSDNDGTYHY
                     YFGQKHLSGHLSDNVLFGLALIDLYNVTGEKRYISKAEDISHLIINKFYDKTNNRFIP
                     YLETTIINPAITSALSDYNTYLSNYRAVIFLNRLYDYNGNEKLKGIIESVNSRLRTVY
                     EQYGPSAALYGTALRWSLETPLVVTIIARDKNVGRFLLQVNKMYIPQKVVKALSLDRE
                     KDRIRTFGYPIEEAAYACSGKRCSPAFTEPDSLIAEIKKFMENLNRKDKMN"
     gene            complement(14246..14923)
                     /locus_tag="VMU21_11720"
     CDS             complement(14246..14923)
                     /locus_tag="VMU21_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78231.1"
                     /translation="MRLRAPGSGPITVAVFAGKERAIRLLEKLQTTGFEASVLTADDI
                     EKEDRAFIVRRFSLGERDLGATAEKGDSISIPFQEIDIILRGTAIIRDVTTDTVKNRS
                     VSPGRAVLSGGLMATKTTKNVREVITEERQGFANLYARDGSTLAFRENTLVYESLGSA
                     LRPSHALNFTYLISELRRRCPDAQYDERLLSRAGQVALLGPSLNPEEHLVVATALLRK
                     VLGTKIG"
BASE COUNT         3845 a         3663 c         3464 g         4073 t
ORIGIN      
        1 ctaaggtgcc accgctgaac tacggtgtta tgccacatta ccctcggaaa tccgcattag
       61 ttgcaacttt tttaaagtga agacttcggt gttatcaaca ctcgcaggca tcctttttcg
      121 ctaaatcaat ttcccttagg ctatcttccc cttaaaggag attcctttga taggacgatg
      181 ataagtcaag gtcgaaagcc ctacagggca gactgcaagt ctgaaccttt acttatctga
      241 gtccgaaaat gcaataggac atgggggaag gtagcctatt tgttataatg caagatgaaa
      301 agcagaaaac cttctcatcc ctcataggaa gataaaatcg accctgcctt cgacaaaatc
      361 ataaagcact acattcaatt cgttgaactt gtcggcttgc ctgccaatac cggctggatc
      421 ggcctaggag atgaggcatt gaaactttgg gaaaagaaag gtatgcaggg atggataaaa
      481 cgttatgaaa aaaatatgac aactgaaaag aacaccatta ttaagtggaa atttcaaagg
      541 ggcatcactg aagaagagaa ggatgcggcg attaaagcag gattagccaa gatatcaaat
      601 tatgcaggcg taaagcattc atctgcttcg gagatcgcaa caaagaccgg ggatatcttt
      661 aatacgtcca agttcctcgg tcatcgcagc ggtattcaaa atacgatgaa gcataccgaa
      721 agaagggaca ttgaaaagct gcgaaagctt caagacgaga tagtcatacc ccctacgctc
      781 atcagtaatt aagacttttt catcccacta cgatgttagt tcattcttgg attgttgggt
      841 agcctccgga atcaaggcgt gtcttcagcc cttgggcgta accttgccgg tgaatgactg
      901 ggcaacgatc ttgccgtcct gtacgacgaa cgtgtcggtt gccagttcgt agacattatc
      961 ggccgtctga gctgtccaaa ggatgtaggc atagtcgcct tcgaccgacc gcagcttcat
     1021 ggtgaacgtg gccccaggtt ttccgaactc cgcaagcagg tgctcaaata gcggtctgat
     1081 ggcgtcgggg cccttgagtg ggccattcgg cgtaaagaaa accgcatcca ttgcgtagtc
     1141 agacagaata cccgtgaggg ccccctcgcc aaagcacttc aagtggcgtt ctagcacgtc
     1201 cttcgtcgta gccatctctt gaatcctcct gcgaacgcta tcctggtgtc cagaagggtc
     1261 cctccttacc tagcttcttt agcgacaaga tcaactcgga tgcatattcc ttcagtcgag
     1321 agagatagtc cggctgtaga cgattcctgt gcagaggccc gctaaacgcc attgcccaat
     1381 tcgacaaggt catgatggac tcagccgaaa cggaatcata aagcgaccaa ttcttcacgt
     1441 gctcttccga ccggaagaga ttcatgcggc ttcaagccgc accccaggtc acttctcctg
     1501 ttaaagcctt ggcgaatgga atattcatgt gtcctacggc cgttggtgga ttgacttcga
     1561 ggatctcctc gtcacgcatg cggatgcgga taggctgccc acaatccagg caactggtgt
     1621 cgatatggat ctccgtacca gggaagagcc agcgcacggc cagcacttcg attcctcact
     1681 gtccgtacca cttttgttcc cccttgatcg ttacacgatg atgtgtcggt acgttggaaa
     1741 aaggcgccca tgactcgaca tagtctgtgt ctttaataaa ccagcaggct atggaggact
     1801 cggccgcctt atgctgcacc tgtctggcct cttccggtcg caccttgaga attcccgcaa
     1861 gctcggtata gtgcggtgcc cgcccagtct tcacaaaata ctccacgaca gccgtgtaag
     1921 cctgttgtac caatgcgtct tgcatatcac tgcctccctc ctgtgtctta taccattctt
     1981 tatgactccc ttctgatatg tgtttatcac aagatcttaa gcttgtcaac cacaacagca
     2041 aaaaatatgc aggggtcgtt tgacggtact acggagttac gaattttgtc gatttccatt
     2101 cgttatcctt agtcttactg tacttctcac tgctgtcagc tacaggaaag agatgtaact
     2161 ccttggcaag gctctagtaa actgttttaa agccactcat attacccttg cagtaacgca
     2221 acagtaacgc aaaatatggc tccttataac ctattgaaat agcgtgataa aaagagggtt
     2281 gtacctcgca aacccttgat tttcttgatg gttataagtc cctaaattta aaagacaaaa
     2341 tcagtttgac ggcttatagt acatgagctg gcgcgcgtag ggcgaaacgg catcaatagt
     2401 cccctgcatc tcttccctgc ccatcgcgtt gaacgcgcgt gcgaatccca cattttcctc
     2461 aagctgcctg atgctgtcac agcctatcac tgccgtcgtt atggggtgcg atagtgcgaa
     2521 tcggaaaaaa ggctccaggg tcccatatcc gggtatcctt tgcgcaaggc ccctgagata
     2581 ggccttcatg ccgacgattc ccatcccctt gccctgggca ataggaatta cctcgtcaat
     2641 aaagctttta tataccggct ctgcgggatt taccggaata agcactgtat caaaatcgaa
     2701 gtcctgaatg cacctcttta tgattgatgg gtcgtggtgg cctgtcacgc caataaacct
     2761 cacaagaccc cttttctttg cgtcagcaaa tgcttgcagg gcaccatcag ggccgaaaac
     2821 ctcctttata tcttcttctg tcctcatgtc atgaacctgc cacaggtcaa gatgatccgt
     2881 catcatctgt ttcagcgttt catggagatg attgagcgcg ccccttctgc cccttgcatg
     2941 ggatttactc gcaaggaaaa tctctcttct tctttcctta agcgctttgc cataatacaa
     3001 ttcactcccc gaatatgccc tcgctgactc gaaataattc actcccaggt cgatcgctgt
     3061 attgatcaat tgagcggcct ctttctcata gccataggtc ctgagaattc cttcacctcc
     3121 gagacccatt acggtaacct cgatacccgt gttgcccagt gtccttttgg gaatttccat
     3181 acaggaatta tatcagacat tccttctgcg ggttattcct gacactttct atctcagatc
     3241 cctgtagggt aaacaattgc ggcacgtgag tggagagcag gacgcctctg tcacttctta
     3301 cagtaaatgg agaaactcgc tcagccttga tctgtcatct tcaggcatat caagaaactc
     3361 aaccccgatg tcataatgct tcgatcttct ccccgggatt tcgatgcagg aggccaccct
     3421 cccgcgaaac ttcagtggcg cgtttccatt cgggaggaaa agcgccatgg gcgccctcca
     3481 ttcgagcttg attccgttat atccttccat aagcattccg ctaaggctga ttttctttac
     3541 cggtgaagtc ttgtaataat agaggactgc gccctcagtt gcgttgatcc tgaacctcaa
     3601 tgcggttggg ggatattcat cgagtacctt ctctccatcg ggaataccga acgttctcat
     3661 gaaatccttg agtttcacca atttatcaga agaaatattc ttaaacataa ctcctgccct
     3721 gtaaaccggg acagcttcct ctctcacgtt cttcacactt ccgccgaaag ttacccagac
     3781 cacaacccct tgaagggtaa aggccatttc tttgttgtga agtttgagga ggtgttggtc
     3841 acctgttttt agactcttct ttgctataat gcacgcccct gtcatgctca catccagaag
     3901 ttcaatctct gtagcaaaga gcgttttggc ttgaagggcc atatcttcta ccgcgaatct
     3961 ctcataccgt cttgtccatg tattcatgag cgaatattct acctgcatga tataccagat
     4021 tctactcttt cagtttatca caacccgcaa taaatgcaag atccttgagg agggactttt
     4081 gcatgacctg cagaatcttg aatgcttacc tggtgttttt tcgaattgct tttgaggaat
     4141 tccgctttat gtgtctcttt attaatgaca aacattttct cattatatat aattgaataa
     4201 ttagttgatc cccaactctg aacaagagaa tgggagttga gatagtagtg tattaactct
     4261 ttgcaaggag gataccatga aaaagcttag tggcctgttc atcatcacga tgttgaccct
     4321 ctccctcgca gcccttttca acggatgcgg tgggggaagc agtccaagca cgtcaagagg
     4381 aggggggacg gatgcaactc ttacggcgtc gaatgctccg caggtagccg acgtggtaag
     4441 gcaggcagtg aaactggtgg cccctgccac attggggcag gtaaatgcca ctagtgtttc
     4501 acctgaaaag agagcgccat tgatgagcat cttcgagaaa gttgcgcctg tcattggaaa
     4561 gcacgcgaca aatggaaaac agtcgagcga tatactaccc agcgagaatt gctccggtgg
     4621 gggaagcatt gaggtaacga atataagcgt ccctgactta tcaggcaatg tcaaagctga
     4681 tgtaaatgtt aacggatgca ctatagggac agaaactatg aacggcgtct tgaaagtgac
     4741 tgtaccggtc ggtgcgctta cagatattct gcacactgaa aagttcacca tcgaggttac
     4801 aagctttacg tactccgact cgactacgac gagtctatct ttgaccgata attttaccat
     4861 actggccatg gatttcacat acgacgggaa tagtctgaca ggcggctcgg tcactcttgg
     4921 agggactgta tcaggtacaa tacatggcaa ccccgttaat atcagctgtg atagcctggg
     4981 gtctcatttt aacgtcaata gttcaggtgt ggctgtatca gtttcaggga ggatgagcac
     5041 ctcatgcctt ggcggctggg tgactttgag tacgaattta cccgtattcg ttccggcgga
     5101 tgccaattgc cctacgggcg gcgaaatagt tgtctccgca gggggaagca gcgttacaat
     5161 gcatgttgag gcaaattcca agatagatat tttcttcaac ggtgctcttg ttcagaaata
     5221 cgatgactgc agccaagtga taggcctctg tacaggatag ctgactaggt taggggatgc
     5281 gaattgcatc ccctaacctc cttcactctc aacaccttcc ttctctttca tcatttccct
     5341 taatcgtagc gcattcttca tggaatagcc taagtggcag ttgttaaaca tcacaaaggt
     5401 ctttgctgcc gtactatcaa ttttcacgag agagggaaca aaactcttca gctcttcatc
     5461 tgagtaaagg taatcatacc tcagggaggt ctcgatcccc ttcctgagcc aggtttcctt
     5521 attccttccg tggagcctga agtaggcggt gtccgtggta acctgcggaa tgaaagggac
     5581 ggttgaaagg ttgccatatt ggggctcgtc agcagtgatg taggtgattt cattttctct
     5641 caaaaaagaa aatacgccat ccctcctgtc aggggtgagc cagcttccgt gcctgaactc
     5701 aaccccgacc tggaaatctt ccatcatgtc tttacaaaga agaatatatt caaggttctg
     5761 gggcttgtac acaaaatagg gggggtactg aaacacaaca actcccgcct tgtgtgtttc
     5821 tgcaaggggg ctgagcgcct ccttgaaaag tctgaatgcc atctggagga attttttgtc
     5881 ccttagggct actcgatctt ttttcaggga gtctgtggag agcatgtctc tgacctcggg
     5941 aggggtagtc ttcaggtcag cgctgtgtcc cgtaagaagg gaatatgcct tgatatggaa
     6001 gagaaagccg ggtggcgtcc tctccgccca acggctcacc atttctacgg agggtattgc
     6061 gtaatagctc gagtccacct caactacatc aaacgtgctt gagtaatatc ttagtctctt
     6121 ttcggcggta ttaacccctt tgggatagaa ctccctgctc ctgatgagac tcttttctgt
     6181 ccatgaacat gagccgtagt agatcatacc ctttgatcaa gcaggatgac cgcgaacatg
     6241 aagatgttct cttcctcttt gctcagacat ttactaatta tagcagaaga tttttattct
     6301 ttttctggtg aaaagcgcaa gcaattgact gatgaatatt tgcatgatat gcttctatta
     6361 atgccggaaa agataattga atcgttcagt gtaaaacgtc tcgatatcct ggatgagcag
     6421 ggggaggtgg atacatccct tatgccttcc cttactgacg atcagataaa gagaatgtat
     6481 gaggtgatgc tcctcgcaag gacatttgac cagcgtgccc tgagcctcca aagggagggg
     6541 aggatcggca cgtatgcttc catcctcgga caggaggcct cacagatcgg aagcgccctc
     6601 gcccttgaga agactgactg ggtctttccc tcattcaggg aaaatggtgt gtacattacc
     6661 ctcggtcatc cgccccatat gatcctgcag tactggtccg gagatgagag gggtctcagg
     6721 acaacagagg ataaaaacat atttccggtc tgcattcctg tggcatcaca tcttccccac
     6781 gcagtgggag cagccatggc agcgaagtac aggaaagaca gaaccgctgt ggccgcatac
     6841 ttcggagacg ggggcacatc caagggcgac ttccatgaag ccctcaattt tgcgggcgta
     6901 ttcaggctgc ctgtggtctt tatctgccag aacaaccaat gggcaatatc agtgcccaga
     6961 gaaaaacaga cttcatccaa gaccattgcc caaaaggcca ttgcctacgg atttcagggc
     7021 attcaggttg atggaaatga tatttttgcg gtatacaggg caacaaagga ggctctggac
     7081 agggcaaagg ggggcgaagg acccaccctc atcgagtgtt tcacctatag ggtctcggac
     7141 cataccactg ccgacgacgc atcaagatac aggaaaaagg aagaggtaga ggcatggaag
     7201 gcaaaggacc ctctcctgag gctgagactg tatatggaga aaaaggggct ctggacagcc
     7261 gagtatcaga aggaagtgga actgagagca agggctgagg tggacgaagc ggagaaaaag
     7321 gccgaatcca ttgcacctcc cgatccgcgg gacatgttca cctacactta tgcggcgctt
     7381 acccagagat tgctgcggga gattaaggat ttctgatatg ccgaagctga acatggtaca
     7441 ggcaattaac cttgccctga gagaagagat gcagagggat aaggatgtcc ttatcctcgg
     7501 agaggacgtg ggaagggatg gaggagtttt cagggtcact gaagggcttc tggaacagtt
     7561 cggtcaggaa agggttgtgg atactcccct tgccgagtct gtcatcgtgg gtgcggccat
     7621 agggatggcg tcatacggac tgaggcctgt tgccgagatc cagtttctcg gatttatcta
     7681 tcctgcaatg gaccagatat ccaatcactc ttcgagactg cgctcccgtt caagaggaag
     7741 atacagttgt ccgcttgtga taagggcccc atacggcgcc ggaatcaagg cacctgagca
     7801 tcactcggag agctgcgagg cgctcttctg tcacatgccg ggcataaagg ttgtgatgcc
     7861 ttcgtctccc tattacgcca agggacttct catctcttcc atcagggacc ccgaccctgt
     7921 ggtattcctc gaatcaacgc ggctctatcg ccttatcaag gaagaggtgc cggagggaga
     7981 atataccgtg cccctcgaaa aagcaaggct ggttcaggaa ggcaaggatg ttacggtaat
     8041 ttcgtgggga ttcatgttac acagggtgct ccaggcagtg gagggctttg atgccgaggt
     8101 aatagatttg atgaccctca accccctgga cgaagagaca atttacggat cggtaaagaa
     8161 gacggggagg gtcgtaatcg tgaatgaagc gcccaggaca gggagcttcg gttcagagct
     8221 gtcggcatcc attgcagagg atgtgatgct cttccttaag tcaccggtca tgagggtgag
     8281 cgcgtatgat acggtacttc ccctccccag gcttgaggat cactatatgc cttctgtgga
     8341 aaagatacgc aaagccattg ctgatgtaat gaaatattag ttctctctct tccgccttgg
     8401 agaacacgct ggcccgttgg gaaaacaact tgcaaaatct tccattatgt agttgaatga
     8461 ttacatgccc gctacaggcc cggatgaact ctttgaagct gggttagaag cccttgcccg
     8521 tggtgattcc ctttctgcca ttaccttctt tgaaagggcc gttcagatgg aagacaggcc
     8581 tgccttctcc tgttatcttg gtctctgtat cgcaaaagaa cggggacagt tccaactggc
     8641 ggtaaccctc tgtgagaagg caaaggcaag agagccccat aacccccttc actatctcaa
     8701 tcttggcagg gtctatctca ttgcagggaa aaaagacgac gcgatacgga ccttccggga
     8761 agggctggga tacgaacaaa gccagccgat cattgatgaa cttgaaaacc tggggacacg
     8821 caagccctcc attatttcct ctctgaagag aagcaatccc gtgaataagt accttgggat
     8881 cattcttaag aggctgggct tgagatgata acggttcacc actgttccac ggaatatccc
     8941 tttttcttca ggagttcaat gattcccttt ttccccacca gatgccctgc ccccaccacc
     9001 acgaaatagc ggcctccggt cttcaggaaa ccttcaatcc ttgatgtcat tgtgttattt
     9061 ctctcataga tgagcttttc ataaattgga agcatacgtg gatcgtcctg tactcccttt
     9121 gatacaactg actcaagggc ttccgtatct ccggtgctcc acgcctttgt cagcgtatcc
     9181 atttctcctg aaaggatatc aaggtccctg agtgtataga ggagaaacga ctcctgatct
     9241 ccctcggaga gtgtgctgag aaggttcatc tgataggtga cgctctccag ttccataacg
     9301 ctttttactc ccacggcttt tttgaggaag tagctgtcaa ttccgtattc cggatcgaac
     9361 ccgagccttt gcatttccag ggccgttatc atgagggcaa gaaaccaggg tttgctctta
     9421 tgaaaaaact caacaggcat cccaaaccta ttcagcctcg cctttgcaag ttcgtacgtt
     9481 tctttggaga gatggctttc caacccctca ttccccatat ataacgcccc ttcaagcatt
     9541 ctcatgattc cttcaagggg catctcgttg atgttagctt ccaccactaa cacatctgat
     9601 ttactaaagg cgtcctcgat cttccccggg agaggataca tctctctcct taatgcatgg
     9661 agagagccca ggatatacac ggtactggat tttgactgaa ccttccagag aaagcttttc
     9721 tggtgatcga gggcataccc ttgagaaaga gaggaaaaaa ataacaaggc agcaatgaaa
     9781 caaagaaaca ggagtcggaa agctttccca tgcattctca tccttttcat cctgcctcac
     9841 ccgttttcat agtcaaagga gccaaaaaga taccttagta cttagtacca tcacctttcc
     9901 tgtccgttat tattattatt atgaagtatt tctgttctcc gtaaagaaaa gaggggttcc
     9961 tgtatggatt caccacgaca tacagggcac ttgcccggtt ttttcaactt ctcccgtttt
    10021 ctgaagacaa aaccgcactt cttgcactca gcaggagtca tcgcaagatg ataatcgccc
    10081 cggctgacac tcctctggat atgttcgaga tgctcaaata cttcctttac ggagatcccg
    10141 acttcgccgg atatgtcttt ggctgagaaa gaattccctt caagaatatc cattatttcc
    10201 ttacggatgg tctcgtgcct ttcctgagga atgaacgact ctttcggctt attcttcaac
    10261 gctccccgca taatcactgt tttgatctca actgctcccg aatacatctc caacaaagtt
    10321 ctaaatcctg tacaaattat atcaattgcc gaatgaaata cctacccaag gactttgcag
    10381 cagctatttt tcgccggaaa gttgaatctc aaagaaaaaa actgagaatt ttcataaaaa
    10441 acacttttct ttcgatttta agcaaggaat cggcctgata gctgccccaa gacaatcagt
    10501 atctaagttg cttatatatg caattggtta tatgatggag atataaggag taagacttaa
    10561 ggttttactc tttgtagcag agacaaaaag aaaggtggtg tagaacatgt ttgacagaaa
    10621 aaacataaaa agcgttttgg cagtgatcgt tgcagcgctc ttagtaacag cgtttgcata
    10681 tcaggcttca gcatactctg ttggcgataa cacgtcatac acctatacag gaaaggttgc
    10741 agaggttgac aatgtttaca aatatgtgac cgtgcaaaca ggatcgaata ccacattcag
    10801 ctttaaactg gatgattatg cgacagtcat gaagtgtgac aagatcgggt ccttagctga
    10861 cctcaagcct ggtgatctgg taacagtata ctatttccag gaaaacttag gcggcagaca
    10921 tatcgcgagc gaggtcgatc tgacggcata cggcatgaag agctgttaat ggctgcacaa
    10981 gagaaaaaaa ggagcgcagg taattctctg tgctcccctg ttctatacac tcaattgttc
    11041 acctgatttg accacattaa tcttctttaa aatctacccg cggtttttga gcgttccacc
    11101 gactgatcga tactttcaag aaactgcaac ttgtgaggat ttacgagaat actgcgcaga
    11161 acgtcgccgt tcagctgcaa cgccttgtta tgttggcctc tatggcaccg ggcggctgtt
    11221 tccgatcgga attatctcgt cgctttaaat cgatgggact caatcgtctc ttgaagagca
    11281 ccaaggattt cctgacattt cgcggagatt aacctcttct ttcccagaga taatgctgat
    11341 gatttaatat ttgtgttcaa tctagacaat ctcaccactt cagcattgat ttccacgatg
    11401 cgtttatatg cagattccgc attgaccaat tccgcactac ctctcaattc aggaatgctg
    11461 cgaagcgcat gaagagcctt ttgggcagtg tctaaatagt ccttcatctt gcgctcaatt
    11521 cggtccatct cctggtcatc aggctcctcg atatgtgctt tatgcaacgc gaacaactgg
    11581 agaatagcag tgattgattc gtaggaatgc acagtgataa agccaggccg attatctcgc
    11641 atatcgtgtt gaatcaaagt tctcaaactt ttctcaaagg ctgccacttc acgatagaac
    11701 tgggtagaag ataatttctg cgccttcaag tttgaattct gagtggcaag ttccaaaatc
    11761 tcttcatcca actttctaaa ttctatccaa cacgagttaa attcagcgac agtctcctgt
    11821 tcttttgaag tgccggtttg ctgaatgatc gctgccaggt cccgcctagc gttttctact
    11881 ttgttcgaag cgcttcgagc ctcgtcagca tacgttttcg atgcttcatc agtaatagcc
    11941 agtacagcat tcttctcggc ctcgatagcc tcaagcaaat gaattctcag tgacgacaca
    12001 atttgaatct tcttcaattc gccctccaag ggcgtgattt tatctttgta aagtatgcca
    12061 atgaccgtaa cactacatag caacatgata gttaccagcc acgataacag tgcgtatgac
    12121 tttctcgttt ttccttgttc catgtccaag actcggcttc ccccataacc taattctgct
    12181 aataacttac actattttgg gttctattaa aagagcgtat tccaggaaga aaagagtatc
    12241 agttcatttt atcttttcga ttaagatttt ccataaactt ctttatttct gcaatcaggc
    12301 tatccggctc ggtaaaagct ggcgagcatc tctttcctga acaagcatat gcggcctcct
    12361 ctatcggata gccaaatgtc ctgattcggt ctttttcccg gtctaatgag agtgctttaa
    12421 ctaccttttg gggaatgtac atcttattga cttgcaataa aaatctgccc acgttcttgt
    12481 cacgggcaat gatcgtgacc acaagtggcg tttccagact ccatcgcaac gccgttccat
    12541 aaagggcagc agaaggtcca tattgttcat agacagttct taaccgggag tttacgctct
    12601 caataatgcc tttcagcttt tcgtttccat tataatcata aagcctgttt aagaatatta
    12661 ctgctctgta atttgacagg taagtgttgt agtctgagag tgcacttgtt atcgccgggt
    12721 tgatgattgt tgtttcaaga tacggaatga accggttgtt tgtcttatca taaaatttgt
    12781 ttatgataag gtgagaaata tcttcagcct tgcttatgta ccttttctcg ccggtcacgt
    12841 tgtataaatc aataagcgcc aacccaaaga gcacgttgtc ggagaggtga ccgctcagat
    12901 gcttctgtcc aaagtaataa tgatatgtac cgtcattatc ggaatagaga tttttcaaga
    12961 gaaagtctgc agttcggacc gccattctga tgagtctctt ttcgctagtg gccccatagg
    13021 cggcaataag gccggttatc atttgggcat tggagtctgc ataaatagta ctatcaacat
    13081 atggcggctt aaacccggct ctctctgttc tcctgtagta ttcttcaccg gcatcctgac
    13141 tgccgtaaaa agctcccgct tttttgtcat agagatattt ctctacataa gtgatggtct
    13201 tattcaaaac cttcttatac ttgttgttgc caaaaaccat ataggctgat gcataatttc
    13261 tgataagcgc tgcgttcagg tccagcattt tctcgtaatg gggagccgac cagtcagact
    13321 ctgtagaata tctgaagaag cctccttcaa cacggtcata aatctcaccc tcagacatgt
    13381 tctcaagagt cgtggaaata attgtcataa gttcggggtt cctcttgttc aaatagcctg
    13441 agaggagaaa atcaattgcg tcgggattag gaaacttttg ggacaatccg aagcccccat
    13501 gtcttttgtc gtatgatgat tccagcatgt tggttatcct ggcaatatca gaaacgtccg
    13561 gagcagtacc gtccatctgc cggcctgtcc cttttctctt cttcttcttt atcgtttcgc
    13621 tcttttcctt gaatcccttt ttatcctctt tgagcatctc taatgcatct gaaagccacg
    13681 caatcatgga ctcctgggaa atgaagtttc ctccctgcac tatttctccc tcaggcgtga
    13741 ggacaacagt gctgggccag cctccctggt tgtagagatt gtctatatcg ggtctcatgt
    13801 ttacatctac ccgtacagga ataaaattgc tattaatgta atcgattata tcaatagcag
    13861 aataagttgt ttcatccatt acgtggcacc agtgacacca tactgcactt agggaaagta
    13921 gtatgggctt accttctctc tgcgcatcgt caagggactg cctctcccag tgcttccact
    13981 ttatgagatg ggtcttgttg gggcgtggag aaaacctgaa atcagaggca aggacaagct
    14041 gattaaaaag aaaaatgaca agggtagcgc ttatggcaag ttttttcatc ataaaaagaa
    14101 atacgtttca gacagataat gtatattacc agatgaaata tatggttttg tttgccatat
    14161 tgcggcgcgg actgcaggaa gaagttgcaa gtttcatcgt tttctttctc gcttacgaaa
    14221 gtggcctttc tttttcttgt atgtgtcatc cgatcttggt gccgagcacc ttccgaagta
    14281 gtgccgtggc tacgacaaga tgctcttcgg gattgagcga aggaccaagg agagcaacct
    14341 gcccagccct gctcaggagc cgttcgtcat actgagcgtc tgggcagcgt ctgcgcagtt
    14401 cggaaataag ataagtgaaa ttcaacgcgt gggacggcct cagcgcagaa ccaagcgatt
    14461 catatacaag agtattttcg cgaaaagcca gggttgagcc atctctcgcg tagagattgg
    14521 caaacccctg tcgttcctcc gttattacct cacgaacatt cttggtagtt ttagtggcca
    14581 tcaagccgcc cgaaagtaca gcgcgtcccg ggcttactga gcggtttttg acagtgtcgg
    14641 ttgtaacgtc acgaatgatc gcagtgccac gcaggatgat atctatttcc tgaaaaggaa
    14701 tgctgatgct gtcgcccttc tccgctgtgg cgccgagatc acgctcgccg agactgaacc
    14761 ttcgcacaat gaatgcacga tcttccttct caatgtcgtc agcagtaagc acggatgcct
    14821 cgaatcctgt ggtctggagc ttttcgagga gtcttatcgc ccgctctttc ccggcgaaaa
    14881 cggcaacggt tatgggacca ctgccaggcg cacgcaagcg cactactgcc tcataaatcg
    14941 ttgctcctat ggcggctgct cagaccttgg cgagtctttc cttatcctca ttcagtccat
    15001 gtattgctac gatatgcatc gttgccgcag acccagcctt cccgt
//
LOCUS       DATKOU010000247        15221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3417676, whole genome shotgun sequence.
ACCESSION   DATKOU010000247 DATKOU010000000
VERSION     DATKOU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15221
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3417676"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VMU21_11725"
     CDS             <1..370
                     /locus_tag="VMU21_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78232.1"
                     /translation="MGVSDVGGDVVAVASQTVKGAVKGAAEVGADVAMVARRTVDGVI
                     EATKEIGGNVEEVAKVAVGGAIEAAGTIGNTAVKAVKDMLVGVVEGVKEVAGSALPKP
                     RQVPSPARKETESPARGKKR"
     gene            380..3463
                     /locus_tag="VMU21_11730"
     CDS             380..3463
                     /locus_tag="VMU21_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HUO78233.1"
                     /translation="MVQPVHTAVKGRARFRVEGLYRSEKLKKEIESRLSSHNDIKGFS
                     INILTGKVLVFYNSDNTPETIAMLIAGVAGESAKTGKPSPPVQRSTVHDQGEWLPAGN
                     IPAEYPHPEAERTKLIQSRRRLRRSVILSEMQTEEAWHLMDATEVLRTFGTSRNAGLS
                     ADLVSVNLRRFGPNILPEAVPRSGLSMFFSQFKSLPVALLGVAAGISVLTGGLADAAV
                     IMGVVLINAAIGYTTESQTEKTIHALKSLVRPSAAVVREGSMKEIGAGELVPGDLLLL
                     RPGSYVAADARLIETNHLSIDESALTGESMPVNKAVSPLTQKDIPLADRTNMVYMGTL
                     VTGGQGIAVVVATGRFTEMGKIQTLVGEARPPETPMERQLDRMGSQLVFICGIVCGMV
                     FITGLLRGYGLLQMLKVSISLAVAAVPEGLPTVATTTLALGIRNMRKHNVLIRHLNAV
                     ETLGSVQTICLDKTGTITLNRMSVVAAYTGMRDFTVSHGQFFQSGEPLSVYFHDELLR
                     LIHLSVLCNESEVIREDGKYVVNGSPTENALIHVALSSGVDVQMLRQKFPVLKIYHRS
                     EKRNYMYTLHETQDDSALIAVKGSPNEVLSLCTWQMIDGQKIPLTDEDRLALETENER
                     MAGEALRVLGVAYSFTGDKPLLINEDIDSTDNLIWLGLVGMADPVRDGVKGLIGEFHE
                     AGIDTVMITGDQSPTAYAIGKELGLSKGEQLEILDSTHLIDIDPEVMKALCKRVHVFA
                     RVSPAHKLRIVQVLQSAGKVVAMTGDGINDGPALKAADIGIAMGHTGTDVAREVADVV
                     IEDDNLETMVIAISQGRTIYNNIRKSVHFLLSTNLSEIMVMFTAIAGGLGQPLSAIQL
                     LWINLMSDIAPGLALALEPPEPDVLSQPPRDPDEPIVRKSDFKRITFEAAMISAGTLG
                     AYGYGIAKYGMGQRASSLAFLSLTTGQLLHAISCRSERHSIFGREKLPPNKYLNMALG
                     GSFAIQLLAMVIPGLRNLLGIAPISVLDGLVIGGSAVLPLLVNEGTKKLPKEVTA"
     gene            3460..4659
                     /gene="metK"
                     /locus_tag="VMU21_11735"
     CDS             3460..4659
                     /gene="metK"
                     /locus_tag="VMU21_11735"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUO78234.1"
                     /translation="MKKDFIFTSESVTEGHPDKLCDQISDAIVDHFLQQDPYSRVIAE
                     CAVSTAIMFIAARFESKASVDFPNIARQVVTQIGYDQNAFNAKTCSIVTSLKELPHDE
                     HCCFDERELSDAEIDAIPTKNQVTIFGFACSQTSALIPLPLWLAHKLARKLTSVRLQK
                     ILPYLAPDGKTQVGIEYRNGKPSRIHSITVIASQDKSSAAGGPDMKRLEDDIRQAVID
                     SVFPDEDIKPDRSTKIFINPDGPFIIGGPSVHSGLTGRKNAIDLYGEYSRQSGAALSG
                     KDPMRIDRIGAYAARYAAKNVVAAGLAEECEIQLSYSIGLSRPVSLQVETFGTGKILD
                     EKISALVERHFDFRLAGIVRQFNLRYLPSILKGGFYRKLAAYGHVGRMDIGLPWELTD
                     KIQQIKG"
     gene            4682..4948
                     /locus_tag="VMU21_11740"
     CDS             4682..4948
                     /locus_tag="VMU21_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78235.1"
                     /translation="MAEIKGIRENLEDLAFLYEDENEKRLHMNAIQMLAMHVGASPVE
                     IEGVYEMVLSRYKKYAKVRDFLPILVSREVKHLINTKRAIRISP"
     gene            5053..5904
                     /locus_tag="VMU21_11745"
     CDS             5053..5904
                     /locus_tag="VMU21_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HUO78236.1"
                     /translation="MKNKSQWIVLALTIAYFFIELIGGLYYHSLALVTDASFMAINVT
                     GQLIALYIGKLALKLPDKTRTFGYERAKVLSGLFNGMLVGFLIFYVFIEAYQKILHSE
                     PLEADKILLIAVIGLFVNAFGLMKLYKHSADMNIKGALLLILNDTLGSVGVITSSILI
                     KYTHFYFIDPLTGVIIGLLAGYPTYFLIKDSVQILMEGNPAKIATDDVEAFLRNNFAD
                     IHSVKDTHIWGLSPEQIILALRIRTNGTVYHRDTVKMMKSQLQNRFGFADMYIELYET
                     KQEPSDR"
     gene            5948..6262
                     /locus_tag="VMU21_11750"
     CDS             5948..6262
                     /locus_tag="VMU21_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78237.1"
                     /translation="MKSNTWTHVFIMLLIVLTSTVCIVLQKKPGEAQPYREKYITLRS
                     SYVDLARSHAQMLEILLDATPDLQKAIPEYRYLGTTALSETLHRQIKELRMKAETLET
                     EQ"
     gene            6340..7068
                     /locus_tag="VMU21_11755"
     CDS             6340..7068
                     /locus_tag="VMU21_11755"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUO78238.1"
                     /translation="MQVISIANHKGGVGKTTSAVNIAACWGEEGKKVLLIDMDPQGSA
                     SMSFGITNDGDGLLHALQRSAALPVVSTQVKDVDVVPSGPNLVVARQLFTGSLGKELL
                     LRCIKQTQGDWERIIIDCPPSLGVLTVTSLWASKYAVVPVETTYLALRGLTQMVESVK
                     RDRPGIEISAIIPCRTHPRRRIHQEILERLEELFPGRVSPVIRENVALAEAPGLGKPV
                     TLSAPRSHGAEDYRNVALWLSNHI"
     gene            complement(7354..8049)
                     /locus_tag="VMU21_11760"
     CDS             complement(7354..8049)
                     /locus_tag="VMU21_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78239.1"
                     /translation="MNAVAVSEIEHHEGTGKVGITVKDGIIGSLKGINEIEAEIVSLV
                     RNTVSNTLRATGSIAHEAVTVSKDVIKGTIQATEEVGTGLILSTKSVAKGIVMGVSDI
                     GGDVVTVASQTVKGAVKGAAEVGADVAMVARRTVDGVIEATKEVGGNVLEVAKAAVGG
                     AIDAAGGIGNTAVRAVKEILVGVVEGVKDVGGAVLPKPRPETGSPKGHATFTEGEAAE
                     AFKARHKKSRSER"
     regulatory      complement(8119..8197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8453..8638)
                     /locus_tag="VMU21_11765"
     CDS             complement(8453..8638)
                     /locus_tag="VMU21_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78240.1"
                     /translation="MKSAALGKYDSNMLGKLQCQSIGDLWSPIHPKKGIVFFKGEGFR
                     LAAEMAEMSLWISSGFC"
     gene            8957..9115
                     /locus_tag="VMU21_11770"
     CDS             8957..9115
                     /locus_tag="VMU21_11770"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HUO78241.1"
                     /translation="MNKHEEIEALARQLFEESGRAEGRDLDNWLEAERVVYSKHQGQE
                     THESGSSS"
     gene            9430..10203
                     /locus_tag="VMU21_11775"
     CDS             9430..10203
                     /locus_tag="VMU21_11775"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HUO78242.1"
                     /translation="MFEAIVLGIVQGITEFFPVSSTAHLILVPWFFHWSGEVNTLMFD
                     VALHAGTLAALLICFYSDWLHMLLKDRKTLAFILIATIPAGIAGVVFKHLIEDQLRSP
                     LIIACSTVVFAVLMLVAEKYSTRDKGQASFKDWVLIGIAQAIALIPGVSRSGVTITAG
                     LFRNLTRESAAKFSFLLSTPVIGGAALLEGRKLLRAPGDYHLDTFAIGFLASFVSGLF
                     AIKFLLRFLRKHPLNIFAYYRFLLAAVIAVTWATTSFLR"
     gene            complement(10237..10626)
                     /locus_tag="VMU21_11780"
     CDS             complement(10237..10626)
                     /locus_tag="VMU21_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78243.1"
                     /translation="MSFWQKMKKDLQKGIGESIVLVKESAAVVQKKAEELSEEGKKRY
                     KLYELQTKVQKEMTRFGQKVYDLSTDGKDPMIDSKVKAMKAGIAKLQDEISELEGKTV
                     TRKRKTAPKGRTRKKPKETNTTTEEEE"
     gene            complement(10650..10991)
                     /locus_tag="VMU21_11785"
     CDS             complement(10650..10991)
                     /locus_tag="VMU21_11785"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78244.1"
                     /translation="MEYYIHYVPGRIRIETPVLHENPGKAEEFHKFIKGIEGIKEVEI
                     HTVTGSALLLFDERTINCEQVIGILEKYGYFCLSKAETSNQYIEKVTEKVLKIAENIV
                     TTSLGEGGFEE"
     gene            complement(11334..11705)
                     /locus_tag="VMU21_11790"
     CDS             complement(11334..11705)
                     /locus_tag="VMU21_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HUO78245.1"
                     /translation="MSYYIHNVPGRLRVKSPAVKRNQDAADEVKKSLSTLQGIATVDI
                     NLTTGSILVNYNSKTIHHEDIVSLLQRKGYFDPSKALDNDQYIHRAASKAGDVVGRAI
                     FGTFLETALEGSALSLLTFLI"
     gene            complement(11686..11889)
                     /locus_tag="VMU21_11795"
     CDS             complement(11686..11889)
                     /locus_tag="VMU21_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78246.1"
                     /translation="MMCGTQFTGNAGKLSVKGKPVCPVCGKSMHVYKLEGEVIRFRCA
                     GYPVCKTYRKFRMKEEKDELLHS"
     gene            complement(12029..12244)
                     /locus_tag="VMU21_11800"
     CDS             complement(12029..12244)
                     /locus_tag="VMU21_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78247.1"
                     /translation="MYKTQGVICQYLKGSPEGAKCGVVNELIRALQDADIQLCMGRHY
                     EVCSIYVSLLLRNAAETIKCNENVTIP"
     gene            complement(12861..13280)
                     /locus_tag="VMU21_11805"
     CDS             complement(12861..13280)
                     /locus_tag="VMU21_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HUO78248.1"
                     /translation="MRLFKKVIDIIVKMMIPLVILALMIGIARIFLDLRKVFNSSTIA
                     GGFDIMVTNILSMFVVIELLKGIIEYFEIHRVKITFITDATLVFILREVMIGVYQHTM
                     GWVEILSISGLLMVIGGLRTVAIVYSPDKGKEAVEHE"
     gene            complement(13576..14244)
                     /locus_tag="VMU21_11810"
     CDS             complement(13576..14244)
                     /locus_tag="VMU21_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HUO78249.1"
                     /translation="MEEAAVNGNNKRIIVMFTLLLCLMANISQGFNPASFDQMDKTRP
                     HQCVGCDLTGIDIDGWEMDDSILRDANMRRANLAHAEFESADLRGADLREANVFMVVF
                     SRANLGKANLSKLDLRSATINDANLSGADLSETNLKDGTLLGADLSSADLHLSVCSGA
                     NLRYTNLRKANFRRAALDNTDLRGADLTEANLEGADLRGAKLTDAIWTDGRKCTAGSE
                     GVCR"
     gene            complement(14716..>15221)
                     /locus_tag="VMU21_11815"
     CDS             complement(14716..>15221)
                     /locus_tag="VMU21_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78250.1"
                     /translation="QILDGTNGSTAADFDGRHFTKAVDTGWTSRPESDDRDCSARHRW
                     PWSFKKSHKPKDDEMPASNILTLWGMADLGSDQTDVYTLSMSYDNHELLPIHLGKGLL
                     GLATEDENGEWVNAVDKNFGGTKKFILGPWKPGYKLGTYGVDPKTKTAWAVINYNADF
                     AVARFDQ"
BASE COUNT         3923 a         3830 c         3488 g         3980 t
ORIGIN      
        1 catgggtgtg agtgatgtcg gcggagacgt tgtcgctgtt gccagccaga cagttaaagg
       61 tgcggtaaag ggtgcagcag aagtgggtgc ggatgttgcc atggtggcaa gaaggacggt
      121 tgacggagtt atcgaggcca cgaaagaaat tggcgggaat gtggaagaag tggcaaaagt
      181 ggctgtgggt ggggcgatcg aagctgcagg cactatcggg aacacagcgg taaaagcggt
      241 gaaggatatg ctcgtcggcg ttgtagaggg cgtaaaagag gtggcaggct cagcgctgcc
      301 aaagccgcga caagtgcctt cgcccgctcg taaggagacc gagagccctg caaggggaaa
      361 aaagaggtaa caaaggggcg tggtccagcc ggtacatacg gcggtaaaag ggagggcaag
      421 atttagagta gaggggcttt atcgctctga aaaactcaag aaagagattg agtcaagact
      481 ctcttcccat aatgatatta aggggttctc gataaatatc cttaccggga aagtccttgt
      541 attttataac tctgacaata ctcccgagac gattgcaatg cttattgcag gagttgcagg
      601 tgagtcggcg aagacaggaa aaccgtcccc accagttcag cgaagtacag ttcatgatca
      661 gggcgagtgg ctccccgcag gcaatattcc ggcagaatac ccgcaccctg aagcggagag
      721 aaccaagctc atacagagca gaaggagatt aagaagaagt gtcatccttt ccgaaatgca
      781 gaccgaggaa gcctggcacc ttatggatgc aacggaggta ctcaggacct tcggcacatc
      841 tagaaatgcc ggactgtccg ccgacctcgt cagtgtaaat ctccggagat tcggacccaa
      901 tattcttcct gaagcagtcc cccgatcggg attgagtatg ttcttcagtc agttcaagtc
      961 cctccctgtg gcacttctcg gtgtcgcggc aggcatttct gtacttaccg gcggcctcgc
     1021 agatgccgca gtgattatgg gcgtagtcct tataaacgca gccatagggt acacaaccga
     1081 gagccagact gaaaaaacta tccatgccct caagagcctc gtcaggccct ctgcagctgt
     1141 ggtacgtgag ggcagtatga aggaaattgg ggctggagag ttagtgccag gagacctcct
     1201 gctgcttaga cccggcagct atgttgcggc agatgcaaga ctcatcgaaa caaatcacct
     1261 gagcattgat gagtctgccc tgacaggtga gagtatgccg gtgaacaaag cggtatctcc
     1321 cctcacgcaa aaggatattc ccctcgctga caggactaac atggtctata tggggaccct
     1381 cgttaccggc ggacagggaa ttgctgtcgt tgtggcaaca gggaggttca ccgagatggg
     1441 caagatccag acccttgtgg gtgaggcaag gccccctgaa acacccatgg agcgccagtt
     1501 ggacaggatg ggcagccagt tggtatttat ctgcggaata gtctgcggca tggtctttat
     1561 caccggactg ctccggggat acggtctttt gcaaatgctc aaggtttcca tatcccttgc
     1621 ggtagctgcg gtccctgaag gtcttcctac agtggcaacc acaaccctcg ccctcggcat
     1681 cagaaatatg cggaagcata atgtcctcat acgacatctt aatgcagtcg agaccctcgg
     1741 ctcggtccag accatatgcc tcgacaagac agggacaatc acccttaaca ggatgtctgt
     1801 ggtcgccgca tataccggca tgagagactt taccgtatct catggacagt ttttccaatc
     1861 cggtgaacct ctgagtgtat atttccatga tgaactctta agactcattc acctatcggt
     1921 cctctgtaat gaaagtgaag tcatccggga ggacggcaaa tatgtcgtca acggctctcc
     1981 caccgagaat gccctgatac acgttgcatt aagttcaggg gttgacgttc agatgctcag
     2041 gcagaaattt cctgtcctga agatttatca ccgttcagag aagcgaaatt acatgtatac
     2101 gctccatgaa actcaggatg attcagcact cattgccgta aagggcagcc cgaatgaggt
     2161 gttatcgtta tgcacctggc aaatgataga cggtcagaag attcctttga ccgatgaaga
     2221 ccgcctcgcc ctggagaccg aaaatgaacg catggcgggc gaggccctcc gtgtgcttgg
     2281 ggtcgcatat tcattcaccg gtgacaagcc gcttctcatc aatgaggaca tcgattctac
     2341 agacaatctc atttggctcg ggcttgtggg catggcagat cctgtcaggg atggagtgaa
     2401 gggattaata ggcgaatttc acgaggccgg aatagacacg gtgatgataa ccggcgacca
     2461 aagcccgaca gcctatgcca ttggaaaaga attaggcctc agcaaaggtg agcagctcga
     2521 aattcttgat tccacccatc tgatcgacat cgaccctgaa gtgatgaagg cactctgcaa
     2581 aagggtgcat gtctttgccc gtgtcagccc tgcccataaa ctccggatag tccaggtcct
     2641 ccagagtgcg ggaaaggtcg ttgccatgac aggcgacggc atcaatgacg gtccggccct
     2701 taaagctgcg gatatcggca tcgccatggg acatacaggc accgatgttg cccgtgaggt
     2761 cgcagacgtc gtcatcgagg atgataacct cgagacaatg gtcattgcca taagccaggg
     2821 aaggaccatt tataacaaca ttaggaagtc agtccatttt ctcttgtcca caaatctcag
     2881 cgagatcatg gtcatgttca ccgccattgc aggaggtctc ggccagcctc tgagtgcaat
     2941 acagttgctc tggatcaacc tcatgtccga tatagcgccg ggcctggcgc ttgccctcga
     3001 gccgccagag cctgatgttc tgagccagcc gccgcgcgac cctgacgagc ccattgtaag
     3061 gaaatcagat ttcaagagaa tcacctttga agcagcaatg atatctgccg ggaccttggg
     3121 ggcttacggg tatggcattg cgaaatacgg catgggccaa agggcaagta gtcttgcgtt
     3181 cctgagcctt accaccggac agctcctcca tgcaatcagc tgtcgttcag agagacacag
     3241 catattcggc agggagaagt tacccccaaa caaatatctc aatatggccc tggggggttc
     3301 ctttgccatc cagcttttag ccatggtgat accggggctg agaaatctcc tgggcatcgc
     3361 cccgatcagt gtacttgatg gtcttgtaat cggaggcagc gccgtgctcc ccttattggt
     3421 gaacgaagga acaaagaaac tcccgaaaga ggtaaccgca tgaaaaaaga cttcatcttt
     3481 acatcagaat ccgtgacaga aggccatcct gacaagctct gcgaccagat aagcgacgcc
     3541 atagttgacc atttccttca gcaggacccc tattcaaggg tcattgccga atgtgcggta
     3601 tcgactgcga tcatgttcat tgccgcaagg tttgaatcca aagcgagtgt ggatttcccg
     3661 aatatagcaa ggcaggttgt aacccagatc gggtatgacc agaacgcttt taatgcaaag
     3721 acatgcagca tcgtgacaag tctcaaggaa ttgccccatg atgagcattg ctgctttgac
     3781 gaaagagaac tgtcagatgc agagatcgat gcaattccca cgaaaaacca ggttaccatc
     3841 ttcggttttg cctgcagtca gacatctgcc cttattcccc ttcccctctg gctggcccac
     3901 aaactggcaa ggaaactaac ctccgtaaga ctccagaaga ttctgcccta tcttgcacct
     3961 gacgggaaga cacaggtggg gatcgaatac aggaacggga agccctccag gattcacagc
     4021 atcacggtca tcgccagtca ggacaagtct tccgcagcag gaggacctga tatgaagagg
     4081 ctcgaagacg atatcaggca ggctgtgata gattctgtat tccctgatga ggacataaaa
     4141 cctgaccgca gcacaaagat atttatcaat cctgacggcc cgtttatcat aggaggtcct
     4201 tcggtccatt ccgggctgac cggaagaaaa aatgcaattg atctgtatgg agaatactcg
     4261 cgacagagcg gagcagccct gagcggtaaa gatcccatgc gcatagacag aataggcgcc
     4321 tatgcagccc gttacgcggc aaagaatgtc gttgctgcag gacttgcaga ggaatgcgaa
     4381 attcagctca gttactccat cggcctttcg aggcctgtga gccttcaggt agagaccttt
     4441 ggcacaggaa agatccttga tgagaaaatc tcagcgcttg ttgagaggca tttcgatttc
     4501 cgccttgcag ggattgtaag acagttcaac ctgcgatacc tgccatcgat tttaaagggt
     4561 ggcttttaca gaaaacttgc ggcttacgga catgtgggca gaatggacat tggtctgcca
     4621 tgggaactta ccgacaagat tcagcagata aaaggttaac cggcgaagtg aggatggtta
     4681 tatggctgag ataaaaggga ttcgcgagaa tttggaggat cttgctttcc tctatgaaga
     4741 tgaaaacgaa aagaggcttc atatgaatgc aatccagatg cttgcaatgc atgttggggc
     4801 gtctcctgtg gagattgaag gagtgtacga aatggttctg agcagatata aaaaatatgc
     4861 gaaggtcaga gactttttgc ccatcctcgt gagtagggaa gtgaagcacc tcatcaacac
     4921 gaagagagca atacgcatca gtccataata aagagaaaaa actgaaagat ataaaccaca
     4981 ttcattggat catgtgcccc tgcgttgacg tgagagtatc catgttgcag ccacacagaa
     5041 agagttcatg gaatgaaaaa taagagccag tggatagtcc ttgcgctcac aattgcctat
     5101 ttcttcatcg aattgatcgg aggtctctac taccacagtc tggcccttgt tactgatgcg
     5161 tcctttatgg ctatcaatgt aacgggtcaa ttgattgccc tttacattgg aaaacttgcc
     5221 ctgaaactcc cggataagac cagaaccttt gggtatgaaa gggcaaaggt attatcaggt
     5281 ttatttaatg gaatgctcgt cggattccta attttctatg tctttatcga ggcatatcag
     5341 aaaatattac attccgaacc cctggaagca gataagattt tattaattgc cgttataggt
     5401 ctctttgtga atgcgtttgg attaatgaag ctttacaaac attccgcaga tatgaatatc
     5461 aaaggtgccc tgctcctcat attaaatgat acccttggct cggtaggggt tatcacctca
     5521 tccattttaa taaaatatac acatttctac ttcatagatc cgctcacagg agtaatcata
     5581 gggctgttgg ccggataccc cacttatttt ctgattaagg atagtgtaca gattctcatg
     5641 gaggggaacc ctgcaaaaat agccactgat gatgtggaag catttctccg taacaatttc
     5701 gcagacatcc acagtgtcaa agacacccac atatggggat tatcgcccga acagattatt
     5761 ctcgctctga ggataagaac caacggaaca gtgtatcaca gggatacggt caaaatgatg
     5821 aaaagtcagt tgcaaaacag attcggcttt gctgacatgt acatcgagtt gtacgaaacc
     5881 aaacaggaac cctcagatcg ctaagggaaa gaaacaccgc cgcttagtaa aggaggcttt
     5941 ggtattcatg aagagcaata cttggaccca cgtattcatc atgctgctga ttgtgcttac
     6001 cagtacagtc tgcattgttc tgcaaaaaaa gccgggtgaa gcacagccat acagggagaa
     6061 atatatcacg ctcaggagtt cctatgtcga tctggcaaga tcacacgcgc agatgctgga
     6121 aattcttttg gacgcaactc cggacttgca gaaggcgatt cctgaatacc ggtatcttgg
     6181 caccactgcg ctctctgaga cactacatcg ccaaatcaaa gaactgagga tgaaggcaga
     6241 gacccttgaa acggaacagt gagccggtca tactctgatc aacggggtcc gatctcttcg
     6301 cagaaaaaac cttctgaatg acagggagaa aaaagaaaga tgcaggtaat ctcaattgca
     6361 aatcataagg gtggagtggg gaaaacgact tctgccgtca atatcgctgc ctgctgggga
     6421 gaggaaggga aaaaggtcct ccttattgat atggaccccc agggaagcgc cagcatgtca
     6481 tttggcatta ccaatgatgg agacggtctc ctccatgccc tgcaaaggag tgcagcgctg
     6541 cccgtggtca gcacacaggt caaagacgtg gacgtggtcc cttcaggtcc caatctcgta
     6601 gtggcacggc agctttttac cggatcattg ggaaaggaac tgctcctccg ttgtataaaa
     6661 cagacacagg gtgactggga aagaataatt atagactgtc cccccagcct tggggtgctc
     6721 accgtcacct ctctgtgggc cagcaaatac gcggtagtgc ctgtagaaac aacctatctg
     6781 gcgctgagag gcctcaccca aatggtagaa tctgtgaaga gagatcgtcc ggggatcgag
     6841 atctctgcca taataccctg ccggacccac ccaaggcgca gaatccacca ggagatattg
     6901 gagcgccttg aagagctctt tccaggaagg gtatcgcctg tgatacgcga aaatgtggcc
     6961 ttggcagaag cgcccggcct tggaaagccg gtcacgctct ctgcccccag gtctcatggg
     7021 gcagaagatt accgcaatgt cgcgttatgg ttatcaaatc atatctgaaa ccagaggaga
     7081 gcatgtgtgc tcagaatata agtatagttg ctgatatttg atccgacgaa cattgaaata
     7141 taaatccagt ctgttccttc tatcttttta attatatttt ctctttggca atacgaaatt
     7201 atcttgcggg catctggatg gctttggaat gcagcttccg atctttatcg ttgtcgtaca
     7261 agcaggcagt tgccataaca accctgtcag gaaaggcgag cagcgcgatt tcgcatcatg
     7321 gtatgatttc tctgctgccc gccttagccg catctaacgt tcacttcgac tttttttatg
     7381 ccttgcctta aaggcctctg ctgcttctcc ttcagtaaaa gtggcatgtc ccttaggtga
     7441 tcctgtttcg ggccgtggct tcggtaaaac ggcacctccg acatccttaa cgccttcaac
     7501 aacccctacc agtatctctt tcaccgctct caccgcagtg ttgccgattc cacccgctgc
     7561 gtcgatcgca cctcccacag ccgcttttgc aacttctagc acgttccccc cgacttcttt
     7621 cgttgcttcg atcaccccgt caactgtcct ccgtgcgacc atagcgacgt ctgcaccaac
     7681 ctctgctgcg ccctttaccg cacccttaac tgtctggctc gcaacagtaa ccacatcacc
     7741 accaatatca cttactccca tgacgatgcc ttttgccacg ctcttggtgc ttaaaataag
     7801 acccgtgcca acctcctctg tggcctggat cgtccctttt atcacatcct ttgagacagt
     7861 tacggcctca tgtgcgatcg agccggtagc ccggagggta ttcgatacgg tgttcctgac
     7921 aaggctcaca atctctgcct cgatctcgtt gatccctttc aggctgccta tgataccatc
     7981 ctttaccgtg attcccacct ttccggttcc ttcatggtgc tctatctcac tgactgctac
     8041 ggcgttcata atccacctct ctttctcatg cttaacgcca ctcaaaaaat aacaaccgaa
     8101 ctgccatatc tcttttcctt cggcagctcg tccatctcat tcctaaagac ccgccgcttt
     8161 ccgcaccctc ttcacaaagg gtttggcttt gtcgggaaat gcccctgttt ctagattagc
     8221 atatccaatc caattgtcaa tttaagatat gttctccctt ataaataggg gctggtcaag
     8281 agcataacac atgcctgaaa ccaaacctgg tactaatgac tacgtcgcgg aataggtagg
     8341 taatgaatgg ttttagtccg ttgcctgagg cgaaactttc ggctctaatt gatgtgacgt
     8401 atgagaagca ttcacgttca gctccgcaca tgcctttcac accttcttag tattagcaaa
     8461 agccagagct tatccacaag ctcatctcag ccatttctgc agcgagtctg aacccctctc
     8521 ctttaaagaa cacaatgccc ttttttgggt ggatgggact ccagagatcc cctatggatt
     8581 gacattgcag ctttcccaac atattgctgt catactttcc cagcgctgca gatttcatac
     8641 aagcttaaca agatcttcat catgcggtaa ttcagatgag ctatgcttga aagcattatt
     8701 acttctgaag gaggtgagaa agtgaacctg catgatctac gagaacaaaa ggacaagcaa
     8761 tatgagatgt gggtattcag ccgcatgaat gacaagaaag tcatgtgtca tctttacaga
     8821 ggtcacaggt gcatcccatc gcatgacgaa agctgcagga gatgtttccg agagtttgct
     8881 gccgcttagc aggagttttg ttataccacg atatagatga tacattgatc attttagtgg
     8941 aaaggaggtg acgaagatga acaagcatga agagattgag gcgctcgcgc gccagctttt
     9001 tgaagagtct ggtcgggcag aaggacgcga ccttgataac tggcttgagg ctgagcgggt
     9061 cgtttattca aagcaccagg gccaggaaac acacgaatcg ggctcgtcct catagacttg
     9121 cgaggacaca agagtccctc tttacaatat ccctctaggg gaattcccgt ttaatagatc
     9181 aaccaggttc caggtgacta cctggaacct ggttgagtga attaatttag agaaagacaa
     9241 gagaagaagc tccagggttc attcacgtct tctttaccta attatgatag acatttttat
     9301 tcctgttcat tgaacgacca taaaaattca tactttttta atattcgctt aacggtactt
     9361 taataaacag ctgttacagt aaagataaca aagaacactt tttaacgcaa aggaggcacg
     9421 tttactcttt tgtttgaagc aatagtgttg ggtattgtgc agggaatcac ggaattcttc
     9481 ccggtcagca gcaccgccca tctcatactc gtcccatggt tctttcattg gagcggtgaa
     9541 gtgaacaccc tcatgttcga cgttgctctc catgccggta ccctagccgc cctgctcatc
     9601 tgtttttaca gtgattggct gcatatgctc cttaaggaca gaaagaccct tgcgtttatt
     9661 ctcatcgcaa caattccggc cggtattgcc ggcgtagtgt ttaagcatct catcgaggat
     9721 caactaagaa gcccgttgat tattgcgtgc tcaacagttg tatttgccgt gttaatgctt
     9781 gtcgcggaga aatacagcac acgggacaaa gggcaagcat cctttaagga ttgggtactc
     9841 ataggaatcg cccaggccat agccctcatc ccgggggttt ctaggtcagg ggtcactatc
     9901 acggccggac tcttcagaaa tctcacaagg gaatcagcag caaaattctc ttttctgtta
     9961 tccactccgg tcattggcgg agcggccctg cttgagggaa gaaagttgct gcgcgcaccc
    10021 ggagactatc accttgatac atttgcaatt ggctttcttg cgtcctttgt ctcaggctta
    10081 tttgccataa aattcctgct tcgctttctc aggaaacatc ctctcaatat ttttgcgtat
    10141 tacagattcc tgcttgctgc tgtcattgct gtgacatggg ccacaacaag ctttcttcgt
    10201 taaccgcccc atagccatta cacatgtgga ttttctctat tcctcttcct cagtagtcgt
    10261 attcgtctcc tttggctttt ttcgcgttct tcctttaggc gctgtttttc ttttcctggt
    10321 taccgttttt ccctccagct ccgaaatctc atcctgcagt tttgctatcc cggctttcat
    10381 cgcttttacc ttgctgtcaa tcatcgggtc ctttccgtcc gtgctcagat catagacctt
    10441 ttgaccaaat cgtgtcatct ctttttgcac ttttgtttgc agttcgtaaa gtttgtaacg
    10501 ctttttcccc tcttcgctga gttcctcagc cttcttctgc acaactgcag cactctcctt
    10561 gacaaggaca atactctccc cgatcccctt ttgcagatcc ttcttcatct tctgccagaa
    10621 actcatacac attcctcctc tggttgaatt tactcctcaa aacccccttc tcccaatgac
    10681 gtcgttacga tattttcagc tatcttcaga accttctcag taaccttctc tatgtactgg
    10741 ttggatgtct ctgcttttga gaggcagaaa taaccgtact tctcaagaat accgatcacc
    10801 tgctcgcagt ttattgtcct ttcgtcaaag agaagcaggg cactgccggt aaccgtgtga
    10861 atctcaacct cttttatccc ctcgatacct tttatgaact tgtgaaattc ttcagccttt
    10921 cccgggttct cgtgcagcac cggagtttcg atccgtatcc tgcccggcac ataatgaata
    10981 taatattcca tttcccgtcc tcccgtctcg gacagcgagg aacagctgta aacaactttt
    11041 attgtagcag ccacagcctt tattctcaat atactttatg tcctaaggca cctatgagaa
    11101 tccactttca acccacaatc tgatgtgatg gtcttgaata gcatcgcaac aaaaccgatt
    11161 ttgtcgtcct ttgcggggaa gtatccaggg gcttgtttgg tttgcgtttg ggaatcaggc
    11221 caccagagac tttagtgatc tgaattatgt tttattgtca ctcaggaaat aaataaggaa
    11281 ggaagtttta tcaggcagtc cgcgaacctt tgcggggaga tagatggaga gatctatatc
    11341 aaaaacgtga gaagcgacaa tgcagaccct tccagtgctg tttccaaaaa ggtaccgaag
    11401 atggctctcc ccaccacatc tcctgctttg gaagcagccc tatgaatgta ctggtcgttg
    11461 tcgagcgctt ttgacgggtc aaaatatcct tttctctgga gcagacttac gatatcctcg
    11521 tggtgtatgg tcttcgaatt gtaattcaca agaatgctcc cggtggtcaa gtttatatcc
    11581 actgtcgcta tgccctgaag ggtgctcaaa ctctttttga cttcatcggc agcatcctga
    11641 tttctcttga ctgccgggct tttcaccctc agtcttcccg gaacgttatg aatgtaataa
    11701 ctcatccttc tcctccttca tcctgaattt tctgtatgtc ttgcacaccg gataccctgc
    11761 acacctgaat cgtatcacct cgccctcaag cttatagaca tgcatgcttt tcccgcatac
    11821 ggggcagacc ggctttccct tcaccgaaag tttccctgca ttaccggtaa attgcgtacc
    11881 acacatcatg cagaggtacc tctgtttgcc ggtccatgca tgtccatatt tgtacaaagc
    11941 atctgaattg caattcgtac atgtgagttt ttcatcgaga tacaatccat tcataagtat
    12001 atttaaccaa atgttcttta cagttttatt aaggaattgt tacattttcg ttacatttta
    12061 ttgtttcagc agcgtttctt aggagcaagg agacataaat tgagcatact tcgtagtgcc
    12121 tccccatgca aagctgaatg tcagcgtcct gcaaggccct tatgagctca ttcaccacac
    12181 cgcattttgc cccttcgggg cttcccttga gatactgaca gatcactccc tgtgtcttgt
    12241 acattatcgc ctcctttctt cctgtttaaa ttatacaatt cggcatgtta caggattgtt
    12301 acaagaagat tacatttcga tgaacaataa aaaagaacct ccagattatc cttaagcgtc
    12361 atgaggaagc tcttctttgc cttcctgcat tacgcaatct ttcgcaatgc cacaccatta
    12421 aagatgaaaa attgagcaat aaacaccttg catttctccc actctccgat caaacagcat
    12481 tttttgtcag cacaatggat catttccggc tcgatacctg ccacttcgca aatcccctgc
    12541 ttgaagcttg aacacatttc ttaatcaccc cctcatacta ttcccctcaa cgtgcacaca
    12601 caggacactc tggattgtga gaaatcaagt ttaccatcga gatgttacag catgatgaaa
    12661 cagaggttaa ggttcgcata caaaacacaa tatggagctt cacggtgcaa catttggcct
    12721 ctgcacgctt cggaagtatt aatgccaaac acctcatatc gccaaaatag ttgcgtcatt
    12781 tccagtggaa cgcaatcagc aaaacacccg caaccagcgc aatagttatg attatggtct
    12841 cgattttctc gctacactct ctactcatgt tctactgcct ccttgccttt gtcaggagaa
    12901 tacacaatcg caacggtcct tagcccgccg atgaccatga gcaggcccga aatggatagt
    12961 atttcgaccc agcccatcgt gtgctgatag acaccgatca ttacctccct gaggataaac
    13021 accaatgtcg catcggtaat aaaggtaatt tttaccctat gtatttcgaa atattcgatg
    13081 attcccttga gaagctctat cacaacaaac atcgaaagaa tattggttac catgatgtca
    13141 aaaccacctg ctatagtcga actattaaag acctttctta agtcgagaaa gatccttgca
    13201 atccctatca tcagagcaag gatcacgagc ggtatcatca tcttaacgat tatgtctatg
    13261 acctttttga atagacgcat atcaataatc aagttaaatg ccggggagtt gaacaagtca
    13321 caaacgcgcc gtatctttac attcaaaact cttcagggca tcgatgggtg atattaacaa
    13381 aaggatgtta cattcttatg aagtacttgt taaattttta ttcggcaaaa ctccgactga
    13441 agattataag gaaggggcct gtgaaaagct cacaagcccc ttccttagga tgagccaaac
    13501 cgtcatctta cagtgcgctt ttctgcttat tactgccacg catatttgcc cctttccctg
    13561 atttaagcgt agtaattatc tgcacacgcc ctcggagcct gccgtacatt ttcgcccatc
    13621 ggtccagatc gcatccgtca gctttgcccc tctgaggtcc gcgccttcca aattcgcctc
    13681 cgtcagatcg gcacccctga ggtccgtatt atcgagagcg gctcttctga aattcgcctt
    13741 gcggagatta gtatatctca gattagcgcc gctgcatacc gacaggtgaa gatctgcgct
    13801 actaaggtct gcgcccaaca gtgtcccatc ttttaaattc gtctcactta gatctgcacc
    13861 gctcaaattc gcgtcgttga ttgtcgcgga acgcagatct aatttgctta gattcgcctt
    13921 gccaagattt gcacggctga atacgaccat aaatacattt gcttctctca gatcagctcc
    13981 gcgaagatca gcgctttcaa attccgcgtg ggcgaggttt gctcgcctca tatttgcgtc
    14041 cctgaggata gaatcatcca tttcccagcc gtcaatgtct attccggtga gatcacatcc
    14101 cacacattga tgcgggcgcg tcttatccat ctgatcgaaa ctggcaggat taaacccttg
    14161 gctgatattt gccatgagac aaaggagcag ggtaaacatg acgattattc ttttattgtt
    14221 tccattgacg gcagcttctt ccatcctgta aatcctcctc aagtgggctt tagttatcgt
    14281 ttttcccgac gcacatgcac gaaatgcagt tattaatttc atcatctaca cacgtgcatt
    14341 ccgtcctggt tttgctattt tcacatcctt cattcatatc gatccatgtc ttatattcgc
    14401 atttatactt atgcccggat ctacatattt cagactgatc agtgtacttg gtttcattat
    14461 tgtcacaatc cgcagctgat ataccgcagc aagagatgat caagccaaaa gaaacaataa
    14521 agaatgttaa tctggatatt aattccacga ttcacctctc taaaagctgt aagtaccgaa
    14581 aaaacaaggc aacacggctt cgcgtcaaag taatcgctgg aaggccgccc gccggaatga
    14641 accggccagg cggcctcttg acaactaaca ctgttaagcc ggacaaagga aatgccgccg
    14701 gattaaagca atttcctatt gatcaaaacg cgcaacagcg aaatccgcat tgtaatttat
    14761 gaccgcccac gcggttttcg tcttcggatc tacaccatat gttcccagct tatagcctgg
    14821 cttccagggg ccgaggatga atttcttggt gccaccaaag tttttatcta cggcattcac
    14881 ccactctccg ttctcatcct cagttgcaag gcccaacagg ccttttccga gatgaatggg
    14941 gagtaattca tgattgtcgt agctcatcga gagagtgtac acgtcggtct ggtcgctccc
    15001 gaggtcagcc atgccccaga gtgtcagaat attacttgcg ggcatttcgt catcctttgg
    15061 tttgtggctc ttcttgaagg accacggcca tctgtgcctg gcactgcagt ctctgtcatc
    15121 actttctggc cttgaggtcc agccggtatc gactgctttg gtaaagtggc ggccgtcgaa
    15181 atctgctgca gtgctgccgt tggtgccgtc aaggatttga g
//
LOCUS       DATKOU010000248         5774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3419803, whole genome shotgun sequence.
ACCESSION   DATKOU010000248 DATKOU010000000
VERSION     DATKOU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419803"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VMU21_11820"
     CDS             complement(<1..1399)
                     /locus_tag="VMU21_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HUO78251.1"
                     /translation="MRMDKLTIKSQEAMQEAQRFAERKGNQELQPEHLLWALLSDEEG
                     ISCQILKKLNVDLTLLQLDVEKEIDRLPKVTGATPLGQLYVSPRLKETLEMAFKEAEH
                     LTDEYVSVEHLFLALLTIGGPCTELLGRFGVSTDKAREVMREIRGTQRVTDPNPEDKY
                     QALSRYSKDLTALAKRGKLDPVIGRDDDIRRIIQVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQRIIAGDVPETLKDKRVVALDMGSLVAGSKYRGEFEERLKAVLKEIEQAEGKII
                     LFIDELHTLVGAGAAEGSIDASNMLKPALARGDLRCVGATTLDEYRKHIEKDPALERR
                     FQPVYIKEPSVEDTISILRGLKERYEVHHGVKIKDSALISAAVLSHRYITDRFLPDKA
                     IDLIDEAGSKLRMEIDSMPVELDEIERKLRQYEIEKQALMKDNSKDAQQKLKKIGKEM
                     AELHEKRDEFRAQWLS"
     gene            complement(1412..1759)
                     /locus_tag="VMU21_11825"
     CDS             complement(1412..1759)
                     /locus_tag="VMU21_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUO78252.1"
                     /translation="MKSQDKKQPLFMISVVSKMLGVHPQTLRLYEREGLVMPQRTKRT
                     RLYSQEDVERLAMIIRLTRELGVNRAGVDIILRLRHRLEILQHEMDEMMQFLEDDLRS
                     DFMKRLKRLFFED"
     gene            complement(1775..2863)
                     /gene="dnaJ"
                     /locus_tag="VMU21_11830"
     CDS             complement(1775..2863)
                     /gene="dnaJ"
                     /locus_tag="VMU21_11830"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUO78253.1"
                     /translation="MPATVNDYYETLGVSKEASQDEIKKAFRKLARKYHPDLNPGDKS
                     SEKKFKEINEAYTVLSDTKKREEYDRFGKTPFEGGGAWYEGAKTPGYEDIFEFGMGDI
                     FGNLFGRTSGAETQHAKGEDILMGLTVSLEEAFTGTQRTIHINREAACQSCHGSGAEA
                     YDTCNKCKGSGMLQSSKGFFRMQQPCPECRGTGKRITRPCRTCGGRGRITSTETVKVK
                     IPAGVDEGSRVKLKGKGNAGIGGGPSGDLYIEITVKPHPIFKREDSDLYVDVPLTFGE
                     AALGAKIEVPTIDGVAVMTIPPGTQGAQKFKLTGKGFISPKTGTRGNQYVTVKIAVPK
                     DLTGKAKEAAEEIEASYKENPRKRLFNR"
     gene            3272..3865
                     /locus_tag="VMU21_11835"
     CDS             3272..3865
                     /locus_tag="VMU21_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HUO78254.1"
                     /translation="MRELFVGIDVAKDTSSAQGIDRNGKGRFSLTFAMDSEGFMQLWK
                     AIAAHSKDLSEVMVAVESTACYHINLYSFFTAKGVSIVVINPLLIANFAKLSLRKIKT
                     DKKDAMTIAQFLLMNRESISQLSLSQDLQDFRDIARERESVCNMLSAHKVAVKRLLQS
                     TFPELERIADLSTRAMQEFIAVFPSARLLNMARPKAI"
     gene            3872..4456
                     /locus_tag="VMU21_11840"
     CDS             3872..4456
                     /locus_tag="VMU21_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HUO78255.1"
                     /translation="MQRKGVGTRLTYTAEDLIRAAKQSIATISPAKELILQGKVRTLQ
                     HLQERYEELTKIMTEYCKASMLEDFEIITSINGISKCTATTFLAEIGCIGNYASYKKL
                     IAFAGIDPTVYQSGRFEGSSRISKRGNRHLRRVIWMMTTCVIQHNATFRAYFTKKKSE
                     GQPFKKAVFATAHKLIRVIFAMLTQRTMFREVCG"
     gene            4642..5088
                     /locus_tag="VMU21_11845"
     CDS             4642..5088
                     /locus_tag="VMU21_11845"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HUO78256.1"
                     /translation="MNPNDPYDLNRFLSAQEGVYERALAELKGGQKRTHWMWFIFPQI
                     DGLGYSPTAKRYSIKSIEETRQYLNHPVLGKRLLECTEAVVALKGGSISEIFGYPDDL
                     KFKSSMTLFEKIAGRGSVFSFALDRYCHGERDATTLGLLEKPIFLE"
     regulatory      5292..5367
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1361 a         1470 c         1358 g         1585 t
ORIGIN      
        1 cgctgagcca ttgggccctg aactcatctc tcttttcgtg gagttcggcc atctcttttc
       61 caatcttttt taacttttgc tgtgcgtcct tcgagttatc tttcataagc gcctgtttct
      121 ctatttcata ctgcctcaat ttccgctcga tctcatccag ctcaaccggc attgaatcta
      181 tctccatcct gagcttagat ccagcctcat cgatcaggtc aatggcctta tcaggaagaa
      241 accggtccgt gatgtacctg tgggacagga cagcggccga gatgagggct gaatccttta
      301 tcttcacccc gtgatgaacc tcatatctct cctttagtcc cctgaggatg gagatggtgt
      361 cctcaacaga gggctcctta atgtagaccg gctggaatct cctctccagg gccgggtcct
      421 tttcaatgtg ttttctgtat tcatcaaggg tggtggcacc cacgcatctc agatcacccc
      481 ttgccaaagc cggcttcagc atattggatg catcaattga gccctctgca gcccctgcac
      541 ccacgagggt gtggagctcg tcaataaaca gtattatctt tccttctgcc tgttcaattt
      601 ctttaagaac agctttcagc ctttcctcaa attcacccct gtatttggag cctgccacaa
      661 gggatcccat gtcaagggca accacgcgct tgtcctttaa ggtttccgga acatcacctg
      721 ctatgattct ttgggcaagc ccttctgcga ttgcggtttt gcccaccccc ggctctccga
      781 taaggaccgg gttatttttc gtccttcggg aaaggacctg tataattctc cttatgtcgt
      841 catcccttcc gatgacaggg tccagtttcc ccctttttgc cagggcagtg aggtccttgc
      901 tataccgtga gagagcctga tacttgtcct caggattggg gtccgtgacg cgctgcgtcc
      961 cccgtatctc cctcatcacc tcccgggctt tatctgtcga aaccccaaac ctcccgagta
     1021 gttcggtgca tgggccacca atggtcaaca gggcgaggaa aaggtgttcc acactcacat
     1081 attcatccgt aagatgctct gcctccttga acgccatttc gagcgtttcc ttgagccttg
     1141 gtgatacata gagctggcca aggggagttg cacccgtgac cttgggcaag cggtctatct
     1201 ccttttccac atccagttgc agcagagtaa gatcaacatt caatttcttg agaatctggc
     1261 aagagattcc ctcttcgtcc gaaagcagtg cccagagaag gtgctcgggc tgaagttctt
     1321 gattgccttt cctttcagca aacctttggg cctcctgcat cgcctcctgg ctctttatgg
     1381 tcaatttatc cattctcata cacttctcct cctagtcttc aaagaatagt ctcttcaacc
     1441 ttttcataaa atcacttctg agatcatcct caagaaactg catcatctca tccatttcat
     1501 gttgaagaat ctctagccga tgcctgagcc tcaggattat gtccactccc gccctgttca
     1561 ctcccaactc cctcgtaagc ctgatgatca tggccagtct ttccacatcc tcctgggagt
     1621 aaaggcgggt ccttttggtc ctttgaggca tgaccaaccc ctcccgctca tagagtctga
     1681 gggtctgtgg gtgtactccc agcatcttcg atacaacgct gatcataaat aaaggttgtt
     1741 ttttatcctg tgatttcata ctcaactccc taggtcatcg gttaaacagc ctctttctcg
     1801 gattttcctt atacgaggcc tcaatctcct ctgctgcttc ctttgcctta ccagtcaaat
     1861 ccttgggaac cgcaattttt accgtcacgt attgattccc ccttgtaccg gttttcggag
     1921 aaatgaaccc ctttcccgta agcttgaatt tctgggctcc ctgggtccct ggaggtatgg
     1981 tcattaccgc aacaccgtcg atggtgggca cctcgatctt tgccccaagt gctgcctctc
     2041 caaaggtcag gggaacatcc acataaagat cgctgtcctc cctcttaaat atgggatgag
     2101 gctttacagt tatttcgata tagagatcgc ctgacgggcc tcctcctatg ccggcattcc
     2161 ccttgccctt aagctttacc cgggaaccct catcaacacc ggcgggtatc tttactttta
     2221 cggtttcagt tgaggttatt ctcccccttc ctccgcacgt cctgcagggc cttgttatcc
     2281 ttttgccggt cccccggcat tcaggacagg gctgctgcat tctgaaaaaa ccctttgatg
     2341 actggagcat accgcttccc ttgcacttgt tgcatgtgtc atacgcctct gcaccgctcc
     2401 cgtgacacga ttgacatgct gcctccctgt ttatgtgtat cgtcctctgg gttccggtaa
     2461 aggcctcttc aagggaaacg gtaagaccca tgaggatgtc ctcacccttg gcgtgctggg
     2521 tttctgcacc tgaggttctt ccgaagagat ttccaaatat gtcacccatc ccgaattcga
     2581 atatgtcttc gtatcccggt gtttttgccc cttcatacca cgcgccgccg ccttcaaagg
     2641 gggtcttacc aaaacggtcg tattcctctc tcttctttgt gtcactcagt actgtatatg
     2701 cttcgttgat ttctttgaac tttttttctg aacttttatc cccagggttc aggtcagggt
     2761 ggtacttccg tgccaacttc ctgaaagcct tcttaatctc atcctgggag gcttccttgc
     2821 tcactccgag tgtttcataa tagtcattca ctgtagcagg catcttcttt gacctctcaa
     2881 ttttttatac ttcatataat acagatttag taggttcttg tcaactctat taagctgaac
     2941 agtaactatt taatcattag ttcaatttta gcacagaact attttgtgtc aaatcgagtg
     3001 gactttttgg taacctgccc aatgtgatct tttcaaggag aaatacgcgt tgcggcttct
     3061 tcctcctacc aacagatatg gtataaagag aaaaagagcg gtaaggcagg agaacgaagt
     3121 ccttcctggg ttagagaacc ctaagtacag tctgttccct gcctccctga gccatgatag
     3181 gatgattggg tctgagagct ctggtaacgc gggaggattg gtggggaggc acaccctgtc
     3241 ttgtcggaaa gccagccaag gaggtaaggc catgcgagag ctttttgtgg ggatcgatgt
     3301 agccaaggac acgtcttcag cacaggggat cgaccgcaac ggcaagggac ggttttccct
     3361 gacctttgcc atggactcag aagggtttat gcagctgtgg aaagcaatcg cggcgcatag
     3421 caaggatctc tccgaggtca tggttgcggt tgaatcgaca gcttgttacc acatcaatct
     3481 ctattccttt ttcacggcaa aaggggtatc catcgtggtg atcaaccccc tgctcattgc
     3541 caactttgcc aaactttccc tgaggaagat caagaccgac aagaaggacg ccatgactat
     3601 cgcccagttt ctcctgatga atcgagagag tatcagccag ctgtcattat cccaggacct
     3661 gcaggacttc cgggatatcg cgagggaacg ggagtccgta tgcaacatgc tgagcgccca
     3721 taaggtcgcg gtcaagcggc ttctgcagag tacctttccc gagttggagc gtattgccga
     3781 tctgtcaacc agagcgatgc aggagttcat cgcggtgttc ccctcagctc ggttgcttaa
     3841 catggcaagg ccaaaagcca tatagaagat attgcagaga aaaggggtgg ggacccggct
     3901 gacttatacg gcagaggacc tgatacgggc ggcgaagcag tccatcgcta caataagccc
     3961 ggcaaaggag ctcatactac agggcaaggt aaggacgctt cagcatctgc aggagcgcta
     4021 tgaggaactg acgaagatca tgactgagta ctgcaaggcg tccatgctgg aggacttcga
     4081 gatcatcacg tcgattaacg gtattagcaa atgtacggcg accacttttc ttgccgagat
     4141 aggatgtatc ggcaactatg cttcatataa gaagctcatt gcctttgccg ggatagatcc
     4201 caccgtgtat caatcaggaa ggtttgaagg cagtagcagg atatcaaaga ggggcaaccg
     4261 gcatctcagg cgggtgatct ggatgatgac gacctgcgtt atccagcata acgccacctt
     4321 tagggcctac ttcacgaaga agaagagcga gggccaaccg ttcaagaaag ctgtctttgc
     4381 aacggcacat aaactgatac gcgttatctt tgcgatgctt actcaaagaa caatgtttag
     4441 ggaggtctgt ggatagatga ctgaacctga taccccaaaa ttacttctca taacttaagc
     4501 tttcgaacgg aaatcattga ggcaagtcac ggtttgttta aaatttatcc ctgcgtcatc
     4561 tggcctaaat ttgtattttt catgatagta taatgaggag gatgagtatt cctgacaaaa
     4621 ccatttcaag aagcaagtga catgaatcct aacgatccat atgatctgaa tcggtttctg
     4681 agcgctcagg aaggcgttta tgaaagagcg ctcgcagagc ttaagggtgg tcagaagcga
     4741 actcactgga tgtggtttat atttccgcag atagatggat tggggtacag cccaactgcg
     4801 aagcgttact ccatcaagag catagaagaa actcggcagt atctgaacca tcctgtgctc
     4861 ggaaaaaggc tgttggagtg cacggaagcg gtggtagctc tcaagggcgg atcaatttca
     4921 gaaatctttg gctatcctga tgatttgaag tttaagtcat cgatgacgct gtttgagaaa
     4981 atagcaggca gaggctctgt gttctcgttc gcccttgata ggtactgcca tggagagcga
     5041 gatgccacga cgctcggatt gttagagaaa cccatttttc tagaatgaga aaactagatg
     5101 caccctaaga catgctacga aatgtcctcc ctatacctga catctgcaat cgtcttatca
     5161 gcgccgaagg tttcaagcag aagtcagtag gataatgacc tcttgtctca acttctaaat
     5221 cagttaccta acctaaaatt ataatttgac agttgcgtct atgtatggta gaagctagtt
     5281 aagaggggaa atcccgacaa agccaaacct tccgagaggg agggacggaa agcggcgggt
     5341 cttacaggag atggccaagc tgccgaagga caaaactgag gtatcctcgg cttttaggca
     5401 aatcccttga aagggagtca gattatgcgg cgttctgctt ttatcgtttt gtccttgatt
     5461 gttattcttg cttttcgggt gtctcacggg gcatggacct cgcttttgat acccctttcg
     5521 gtgtagacaa aataggcaag ctggactgga ttattctcgg tacaagagat gcagacgatt
     5581 acaataatgt ggtaacaaac ccattgaatg gaacaatgta atcagttttg gagaaagggg
     5641 gaacggtatg tcagtcataa ctatttcgcg gggttcttac agtcacggaa aagagatagc
     5701 ggaaaaggtc gctcataccc tggggtacga gtgtatctca cgggatttgc ttcttgaggc
     5761 ctctcagcag ttca
//
LOCUS       DATKOU010000249        10415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1901299, whole genome shotgun sequence.
ACCESSION   DATKOU010000249 DATKOU010000000
VERSION     DATKOU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901299"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..328)
                     /locus_tag="VMU21_11850"
     CDS             complement(74..328)
                     /locus_tag="VMU21_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78257.1"
                     /translation="MTAQEGHREHVYGKLVMAPIVGLLYVIALPFIAIGTVAAVMGKR
                     LAEGLYHVVGNMVSFGWRPLEANLSGKKKERKRREKDSSK"
     gene            complement(533..1897)
                     /locus_tag="VMU21_11855"
     CDS             complement(533..1897)
                     /locus_tag="VMU21_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO78258.1"
                     /translation="MQKSERILIVDDEKVALKNLEHVMKKEGYEVVSTQSGQNALRIL
                     EEQKFDVVLTDLKMEKVDGMQILRRCRELYPDIEVVMITGYATLESAVESMKHGAFYY
                     IAKPFRLDEVRKVVSEAVEKVRLKGENRQLREQIESYQGKVKIITQDAQMQKLLDTAR
                     QIAPTDCNVIITGESGTGKELFARYLHLHSKRSQGPFFAINCGAFTEELLGNELFGHE
                     KGAFTGAATMKKGLVEMASGGTLFLDEITEMSPSMQVKLLRVIQEREVLRLGATEPIS
                     ADVRFIAATNRDLQDAMKSGSFRQDLYFRLNVVSLYVPPLAERKNDIPLLSYYFLKKY
                     SVLMNKEVTEISQEVIDLIMSYDFPGNVRELENVIERGVALSNGRSIEVAHLPEDLRE
                     LSIRTFRKREGKMPSLEEQELSYIQWVLREVGGNKTLAAQILGIDRVSLWRKLKKYGL
                     EASD"
     gene            complement(1900..3387)
                     /locus_tag="VMU21_11860"
     CDS             complement(1900..3387)
                     /locus_tag="VMU21_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUO78259.1"
                     /translation="MSSLRSKITLGYYGTVAIVVVLSLVTFMELRFLGKKIMFGEVIS
                     EFFDTTLEVRRFEKNFFLYGKQDDYAENMKYVSKAQELMERNMEGFGSVATPEQIEVL
                     RADLKQYREFMENYASLPLKTDAQGSIQRTIVEGRIREAGKNIVTAAEEISKTERKNL
                     QSILKKSQGILIFSITALALLAIAMGQMLSRIVVRPLKALEESMRQIAEGRFGMVSIK
                     SEDREIVSLTFAFNRMLKELEVRQRHLVQSEKLASLGTLLSGVAHELNNPLSNISSSC
                     QILMEEIQENDMEYKKELLAQIDEQTDRARNIVRSLLEFSREKEFKKEMLPLRKLFEE
                     TVRFVKGQLPTGISLSLDISDDISIFADKQRIQQAFLNLLKNAIEAIGEEGSVFIKGK
                     KHRAVDKTGETTELYNYMQYRGKCTIGGDTIDVEIRDSGSGIAPEILQRIFDPFFTTK
                     DVGKGSGLGLFIVYEIIEEHDGCIAVKSEPGKGTTFFISLPMKEQ"
     gene            3545..5548
                     /gene="lon"
                     /locus_tag="VMU21_11865"
     CDS             3545..5548
                     /gene="lon"
                     /locus_tag="VMU21_11865"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUO78260.1"
                     /translation="MPIFKKTEGRHHMATIEELRETVLSTKMPPNVRQVVDNELETLS
                     KINPSAAEYAIGLTYIEYLLSLPWNKETEDNLDLHRAEAILNERHYGLNKIKERILEH
                     LAVKTLIMSRQPRILVVDDEEIARKNLEHILKKEDYLVITASNGREALEMMDASDFDV
                     IVTDLKMEEVDGIDLLERATLKSPDTKVIMITAYASIDSAIEAMKKGAFHYVTKPFKL
                     DEVRKVVKDAIEKRLSTLSSRGSVLCFAGPPGTGKTSLGRAIAEALGRQFSRISLGGI
                     KDEAEIRGHRRTYAGAMPGRIIEEIRRSQVINPVIMLDEVDKIGQDFKGDSASALLEV
                     LDPEQNHSFIDHYLDVPFDLSKVMFIVTANVTDTIQDALRDRMEIMEFTGYTEDEKKE
                     IGLKYLVPRQVQEQGLSGHTPQFSPEAMVKIIREYTREAGIRNLEREIASVCRKIATQ
                     VVQQGKETRPVKVTPDLIERYLGPRKYHFEVADEKDRIGVTTGLVRTEAGGDIMFVEA
                     AKMKGNKELMLTGSLGNVMRESAQAALSYTRSNASRLSISDDFFDTHDIHIHVPAGAI
                     PKDGPSAGATIAIALISLFTGRPARRDVAISGELTLSGRILPVGGIKEKILAARRAGV
                     KAVILPIQNKVDLEDLEENVRGDIEIHLVERIEDIIDKVLRSS"
     gene            complement(5584..6012)
                     /locus_tag="VMU21_11870"
     CDS             complement(5584..6012)
                     /locus_tag="VMU21_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78261.1"
                     /translation="MNEGALGPEASLLQRARLHIRGGNRRLRQGKVPAGLVTLYDALI
                     AAMEWYFASPERKNRLVIRNDDNLKDDKVLYRILVRSGVLDGKFDYMAFDTFIEDIIY
                     EKAAEYEYESLAKGIESVMVQLGVMPFNEEEIPPEDPSTF"
     gene            6435..6608
                     /locus_tag="VMU21_11875"
     CDS             6435..6608
                     /locus_tag="VMU21_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78262.1"
                     /translation="MKGFSEKWDRMFSAVTFAEAGEFDTAREIMREQKRDQKRDTQIA
                     RKTNRISAPGIKR"
     gene            6620..7084
                     /locus_tag="VMU21_11880"
     CDS             6620..7084
                     /locus_tag="VMU21_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO78263.1"
                     /translation="MDTLKKKIEEIMSAISFAEEGEFETAREFLKEERRVLLAVRKDQ
                     VDRRTFRYAVNTCKRINAALDILYISPSKAEDPTLQQYLSELTREGINYRLVQRGGCF
                     KQAIIDYTNTIKGILFAVTESSDNLEADCKGIKASKAWKDLKCPLVVVEDSV"
     gene            7139..7396
                     /locus_tag="VMU21_11885"
     CDS             7139..7396
                     /locus_tag="VMU21_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78264.1"
                     /translation="MANSYGSTKKKPIGGMILFGIISFALYAVLLVKQDMVNEYIGRG
                     GFYAFLPILTAFIFSFFHGSFTGKFWTVLGIEAAKRKKEVK"
     gene            7398..8486
                     /locus_tag="VMU21_11890"
     CDS             7398..8486
                     /locus_tag="VMU21_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUO78265.1"
                     /translation="MHDIVSAAANFIQLDVVNVIYLFAVGFVGGLVSGFIGSGGAFVL
                     TPGMMSLGVPGLVAVASNMCHKFPKALVGSIKRAKYGQVDLKLGIVLGISAEAGVLYG
                     AHIQEGIKKAFGDAGSNLYVSVAFVVILAVVGGFILKDAWKTYRSGNMNEGERVTKLA
                     RWVQSVNIPGTMVYFKSLNARISVLFTIPLGFATGMLAATIAVGGFIGVPSMIYVLGA
                     PSLMASATELVIAFVMGLGGSLKYALHGLVDIRLAMIILAGSLFGIQLGAIGTTYVKP
                     FMIKVVMGIIMVIVLFSRGLMVPVYMSQLGLIHTLGETTVKALKSTSFAIMAFALLLG
                     AFIVLRAMWQGRRAEQAATQEALEHGKI"
     gene            8489..9343
                     /locus_tag="VMU21_11895"
     CDS             8489..9343
                     /locus_tag="VMU21_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUO78266.1"
                     /translation="MARYRKLLVAVDGSESSTHALRESFKLATNERSWITVVSVVPPY
                     EGELELVGVRDVKTALKQPCEKALSMASDIAKTERALIKTVCEEGEIYERIVDLADAE
                     NCDLIVMGRKGMSRLEKTLVGSVTARVIGHTQRDVLVVPDNSHIGWERILISTDGSRY
                     SRAAASKAIDFAMSYGGELRIVSVVDVPPEFYAEAPNALDEMIARAKGYTKNLKIEAE
                     SAGIKAETFVGEGEPYRFISDLAIKQKVNTIILGSHGRTGLKRLLMGSVTEKVIGHAP
                     CPVLVARL"
     gene            9581..9721
                     /gene="rpmH"
                     /locus_tag="VMU21_11900"
     CDS             9581..9721
                     /gene="rpmH"
                     /locus_tag="VMU21_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HUO78267.1"
                     /translation="MGTYTTYHPHKIKRKRTHGFLERMSSKGGQKTLQRRRSKGRKRL
                     SV"
     gene            9753..9962
                     /gene="yidD"
                     /locus_tag="VMU21_11905"
     CDS             9753..9962
                     /gene="yidD"
                     /locus_tag="VMU21_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HUO78268.1"
                     /translation="MAKVIILFIKAYQYVFSPLFHGSCRFVPSCSAFSIEALGKYGAI
                     KGMYLSVRRILRCHPFHPGGYDPVQ"
     gene            9920..>10415
                     /gene="yidC"
                     /locus_tag="VMU21_11910"
     CDS             9920..>10415
                     /gene="yidC"
                     /locus_tag="VMU21_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HUO78269.1"
                     /translation="MPSLPSWRLRPGTITMDMDKRTLLAVVLALIVFIGSQYFFSSPA
                     PPPPAQPQKTETKPESSDKGTPAPQVTPLGPAGAEESGPSKDIRIETQLYVAVFSSRG
                     GIIKKFDLKTYKDKDGNPVSLLKAPGVLPPLGLGEKEDFSLSRVNFAVRGTDLILRGS
                     ETGTL"
BASE COUNT         2724 a         2547 c         2442 g         2702 t
ORIGIN      
        1 cattttctcc ctccttgttt tgtgccgtca atgacgacat tttgtttatg aatatctata
       61 cttattcctc tcctcacttg gaggaatcct tttcccttct cttcctctcc ttcttcttgc
      121 ctgaaaggtt tgcctccaaa ggcctccatc caaaagacac catattgccc actacatggt
      181 aaagcccttc tgccaacctc ttgcccatta ccgccgccac ggtcccaatg gcaatgaacg
      241 gcagtgcgat cacatacagc aacccaacta taggtgccat taccaacttc ccgtatacat
      301 gctccctatg tccttcctgt gctgtcattt ccttcacccc tttcttttcc ttatttgccc
      361 tgttgattcc agactaccac accgtaacca caaagataat tggaagaaac tcctagttca
      421 aagggaaatg tatcctatgt gatctgaaga aagttactaa gaacgcgggg cgcaagtgat
      481 aagggaagtg tcaacatcac aagatattaa gaaacgtcct tttcgaagcg actcaatcac
      541 tcgcttccaa tccatacttc ttgagtttcc gccaaaggga caccctgtct atccccagta
      601 tctgtgcggc gagggtcttg ttgcctccca cctccctcaa cacccattga atatatgaca
      661 gctcctgctc ttccagggag ggcatcttcc cttccctctt tctgaatgtc cttatactca
      721 attccctcag gtcctcaggg agatgggcaa cctcaatgct tcttccattg gaaagggcaa
      781 ctcctctctc aatgacattc tcgagttctc tcacattccc tggaaaatca taactcatga
      841 tgagatcaat cacctcctga gatatctcgg taacctcttt attcatgagg actgagtact
      901 tcttcaagaa atagtagctg agaaggggta tgtcattttt cctttctgcg aggggaggaa
      961 cataaagtga gaccacattt aacctgaaat agaggtcctg tctgaaactc ccggatttca
     1021 tggcatcctg aaggtccctg ttggttgcag cgatgaatct cacatccgcg cttatgggct
     1081 ctgttgcacc cagtcgcaag acctctcgtt cctgaattac ccttaagagt tttacctgca
     1141 ttgaaggaga catctcggtt atctcatcca aaaacagcgt acctcctgat gccatctcga
     1201 ccagcccttt tttcatggtt gcagcaccag taaatgcacc cttttcgtgc ccaaaaagct
     1261 cattcccgag aagctcttcc gtaaatgccc cacagttgat ggcaaaaaat ggtccctgag
     1321 agcgtttact gtgcaggtga aggtacctgg cgaaaagctc tttcccggtg ccgctttcac
     1381 ccgtgatgat cacattgcag tcagtgggag caatctgtct tgcagtatcg aggagctttt
     1441 gcatctgggc atcctgggta ataatcttca ccttgccctg atagctctca atctgttccc
     1501 tcagttgcct gttctcgccc ttgagcctca ctttttcaac tgcctcactg accacctttc
     1561 gcacctcatc cagcctgaaa ggctttgcga tgtaatagaa cgcaccatgt ttcatggatt
     1621 ccaccgcaga ttcgagcgtt gcataccctg taatcatgac cacttcaata tcagggtaaa
     1681 gttcacggca tcttctcaat atctgcattc catccacctt ctccatcttt aaatcagtaa
     1741 ggactacgtc gaacttctgc tcttcaagaa tcctgagagc gttctgaccg ctctgtgtac
     1801 tcactacctc gtagccctct ttcttcatca catgttcaag gtttttgagc gccaccttct
     1861 cgtcatccac aataagtatc ctctcactct tctgcatagt tattgctcct tcatcggcaa
     1921 acttatgaaa aacgtggttc cctttcccgg ctcacttttg accgcgatac agccgtcatg
     1981 ctcctcaatg atttcataca cgatgaagag accgaggccg gaacccttcc ccacatcttt
     2041 tgtggtgaag aaagggtcaa agattctttg gaggatctcg ggcgcaattc ctgaaccgct
     2101 atcccttatc tctacgtcga tcgtatcccc ccctatcgtg cacttgccac gatattgcat
     2161 atagttgtaa agttcggtgg tctctcctgt cttatcaaca gccctgtgtt ttttcccttt
     2221 gatgaatacg cttccttcct caccaatcgc ttctatggca tttttgagga gatttaagaa
     2281 cgcctgctga atcctctgtt tgtccgcaaa tatggagata tcgtcagaaa tgtccaggga
     2341 cagggaaatg cccgtgggaa gctgaccttt cacaaacctc acggtctctt caaagagctt
     2401 cctgaggggg agcatttctt tcttgaactc cttctccctg gagaattcca gtaaggagcg
     2461 gacgatgttc ctcgccctgt ccgtctgctc atcaatctgg gcgagcagct ctttcttgta
     2521 ttccatgtca ttttcctgta tttcttccat aaggatttgg caagaggatg agatgttgga
     2581 aagagggttg ttcagctcat gggccacccc cgaaaggagg gtcccaagag atgcaagctt
     2641 ttctgactgg acaagatgtc tttgccttac ctccagttcc tttaacattc tgttgaacgc
     2701 aaaggtgagg gagacaatct ccctatcctc cgacttgatt gataccatgc cgaaccgtcc
     2761 ttctgcgatt tgcctcatgc tctcttccag cgccttgagg ggtctcacca cgatccttga
     2821 aagcatctgt cccatggcga tggctaaaag tgcaagggcg gtaatggaaa aaatgagaat
     2881 cccctgggat ttctttaata tggactgaag atttttccgc tcggtctttg agatttcctc
     2941 tgccgcggtt acgatattct tgcccgcttc ccttattctt ccttccacaa ttgtcctctg
     3001 gatgcttccc tgggcatctg tctttaaggg aagcgacgca tagttctcca tgaactccct
     3061 atattgttta aggtcagctc tcagcacctc tatctgttca ggtgtggcaa cagaaccaaa
     3121 cccttccata ttcctttcca tcaactcctg ggcctttgag acatatttca tattctcagc
     3181 gtagtcatcc tgttttccgt agaggaaaaa attcttctca aatctcctga cttccagtgt
     3241 ggtgtcaaaa aactcggaaa tgacctcgcc gaacattatc tttttcccca ggaacctgag
     3301 ttccataaag gtaaccagtg ataagacaac aacgatcgcc accgtcccat aataccccag
     3361 ggtaattttt gaccttagac tcgacacata tgtcatattt tcattttatc aactgatatt
     3421 gcacaaagca acatttactg aatgttaggc aacatatgac atagcgtatc cttatgacca
     3481 aaaaaagtga gctggaatag aatatgagaa gaatgatatc cttaagagga aaggataatt
     3541 ctccatgccc atctttaaga agacagaagg aaggcatcat atggcaacca tcgaagaact
     3601 gcgggagacg gttctctcta caaagatgcc ccccaatgtc agacaagtgg tcgataacga
     3661 actggagacc ctatcgaaga ttaaccccag tgctgctgag tacgcaatcg gacttaccta
     3721 tattgaatac ctcctgagcc ttccctggaa caaagagacg gaagacaacc tggatctaca
     3781 cagggcagaa gcaatcctta atgaacggca ttatggcctg aacaaaatca aggagcgaat
     3841 actcgaacac ctggcggtta aaaccctgat aatgagcaga cagccccgca tactggtagt
     3901 ggatgacgaa gagattgcca ggaagaacct ggagcatatc cttaagaagg aagactatct
     3961 cgtcattacg gcatccaatg gcagggaggc ccttgagatg atggatgcct ctgatttcga
     4021 tgtcatcgtg accgacctga aaatggaaga ggtggatggc attgatcttc ttgaaagagc
     4081 aactctaaaa tctcccgaca caaaggtaat tatgatcacc gcatatgcct cgatagattc
     4141 tgccattgag gcgatgaaaa agggggcatt tcactacgtt accaaaccct ttaaactcga
     4201 tgaagtgaga aaagtggtga aggacgccat tgaaaaaagg ctgtcaaccc tttcctctag
     4261 aggatctgtg ctctgttttg cgggaccgcc gggaacagga aagacctcct tgggaagggc
     4321 tattgcggag gcactgggta gacaattttc acgtatttca ctggggggaa tcaaagatga
     4381 agcagaaata aggggccacc ggaggaccta tgcgggggcc atgccgggac gtatcatcga
     4441 ggagatccgc cgttcccagg tcatcaatcc tgtcatcatg ctggatgagg tggataagat
     4501 agggcaggac tttaagggcg actctgcttc tgccttgctg gaggtcctcg acccggagca
     4561 aaatcatagc ttcattgacc attaccttga cgtccccttt gacctctcca aggtcatgtt
     4621 cattgtgacc gcaaatgtta ccgatactat ccaggacgcc ctcagggacc gcatggagat
     4681 catggagttc acaggataca cagaggacga aaaaaaagag ataggactaa agtatcttgt
     4741 tccccgtcaa gttcaagagc agggtctgtc aggtcatact ccccaattta gcccggaggc
     4801 tatggtgaaa atcatccggg aatatacgcg tgaggccggc ataagaaacc ttgaaagaga
     4861 gatcgctagt gtctgcagga agatcgcaac acaagttgtt cagcagggca aagaaactcg
     4921 acctgtcaag gtcacgcctg atctcatcga acgatatctc ggtccgagga agtaccactt
     4981 cgaggttgca gatgaaaagg accgcatcgg ggtaaccacg gggctcgtga ggaccgaagc
     5041 aggaggagat atcatgtttg ttgaggcagc caagatgaaa ggaaataagg aactcatgct
     5101 caccggctct ctgggtaatg taatgcgcga gtctgcccag gcggccctga gttataccag
     5161 gagcaatgcc tcgcggttga gtatctccga cgattttttc gatacccatg atatccatat
     5221 ccatgttcct gccggcgcca tccccaaaga cggcccctct gccggagcta ccattgcgat
     5281 agccctcatt tccctcttta ccggcagacc ggccagaagg gatgtagcta tctcgggaga
     5341 acttacccta agcggaagaa tccttcccgt tggggggatt aaggaaaaga tcctggcagc
     5401 acgtcgggca ggggtaaaag cggtaatcct gccgatccaa aacaaggttg acctcgaaga
     5461 tcttgaggaa aatgtgaggg gagatatcga gattcatctg gtggagagga ttgaagatat
     5521 cattgataag gttctgagaa gcagctgatc gttcaaggat gtgatgcgct ttcttctaat
     5581 gtgtcagaat gtcgaaggat cttctggagg gatttcttct tcattaaagg gcatgacccc
     5641 aagctggacc atcactgatt caattccctt tgcgagagat tcgtactcat actccgcagc
     5701 tttctcataa attatgtctt ctataaacgt gtcaaaagcc atatagtcaa acttcccgtc
     5761 aagcacccct gagcgcacca gtatcctgta aagcacctta tcatccttga gattatcatc
     5821 attcctgatg accagacgat tcttcctttc aggtgacgca aaataccact ccattgcggc
     5881 gataagggca tcgtagagag ttacaaggcc ggcaggtacc tttccctgcc gcagccttct
     5941 attgccaccc cggatatgca gtcttgccct ctgaagcaga cttgcctcag gtccaagggc
     6001 tccttcattc atcaatcctg cgtggggcat agtgatcttt aaaggttatc tttcctcaat
     6061 ccgctcaaaa caaggagata aacaatatgc acgatgatta tctatcttat tccttttcct
     6121 ttcacataaa aaaatatcat ggcatatgca gatgtcaatc agcaataccc cataagcctc
     6181 gcgtttcact cttttagcgg ctattgcaat acgcaacatt ctgcatcttt catctttcct
     6241 atggcaaata caccgcttca cgtaccttgc ttctcctttt aaattttgac gttgcaatat
     6301 gcatcaacag ggaatttttg ctgacaaggc acgtgttata ttttgctttt aaattcaaat
     6361 agttatataa atatcatctc ctggcatgag agatgcacta tagaagttaa ctatcacaca
     6421 aggaggataa atacatgaaa gggtttagtg aaaagtggga ccggatgttt tcagcagtaa
     6481 cctttgcaga ggccggggaa tttgacacgg ccagagagat catgagagag caaaagaggg
     6541 atcagaaacg tgacacacag attgcccgga agacaaaccg gatttccgcc cctgggatta
     6601 agagataggg cgcattacca tggacaccct caaaaagaag atagaagaga tcatgtcagc
     6661 tatctcattt gcagaggaag gggagtttga gactgcaagg gaatttctga aagaggaaag
     6721 aagggtgctc ttggcagtcc ggaaggatca ggtggacaga agaacattca gatacgcggt
     6781 caacacatgc aaaaggatta atgcagctct ggatatcctt tatatttccc cttcgaaggc
     6841 tgaagacccc actcttcagc agtacctctc agaactcaca cgcgagggca tcaactaccg
     6901 gctggttcaa aggggcgggt gtttcaagca ggcgattatt gattacacaa acaccatcaa
     6961 gggaattctt tttgcagtta ctgaatcatc agacaatttg gaggctgatt gcaaagggat
     7021 aaaggcctca aaggcatgga aggacctcaa gtgtccgttg gtagtggtcg aagacagtgt
     7081 ttaagtacag cgcaaaaaca gacaccgtat ggcgtcttac attataagga ggtaggaaat
     7141 ggcaaatagt tacggcagta ccaagaagaa acccataggc gggatgatac ttttcggcat
     7201 aatttcattc gcactctatg cagtactctt agtgaagcag gatatggtaa atgagtacat
     7261 tggacggggg gggttctatg catttttgcc catactcacc gcgttcatct tttcgttttt
     7321 tcacggatca tttaccggca agttctggac agtattgggc attgaggccg caaaaagaaa
     7381 gaaggaggta aaataagatg cacgatatag tctctgctgc agcgaatttt atacaactcg
     7441 atgtagtgaa tgttatttat ctctttgcgg tgggatttgt tggtggtctc gtgagcgggt
     7501 ttattggttc aggcggtgcc tttgttctca cgcccggaat gatgagtttg ggagttccgg
     7561 gacttgtggc ggtggcaagc aatatgtgcc ataaatttcc caaagccctg gtgggatcga
     7621 ttaagcgtgc aaagtatggt caggttgatc tcaaattggg gatagtcctg ggtatttctg
     7681 cagaggcagg tgttctctat ggcgcacaca ttcaggaagg cattaaaaag gcatttggtg
     7741 acgcaggttc aaacctttat gtgagcgttg catttgtggt aatccttgct gtcgtgggcg
     7801 gttttatatt gaaggatgca tggaagactt acaggtcagg gaacatgaat gaaggagaaa
     7861 gggttaccaa actggcgcgc tgggtccaat ctgtaaatat cccaggcacc atggtctatt
     7921 ttaagagcct gaacgctaga atatcagtgc tctttaccat acccctcggt tttgctactg
     7981 gcatgctcgc agccaccatt gcggtgggag ggttcatcgg cgtcccttca atgatttacg
     8041 tcctcggagc gcccagcctg atggcctcgg caacagaact agtaatcgcg ttcgttatgg
     8101 gcttgggcgg ctcgcttaag tatgccctgc atggcttagt ggatatacgt ctcgcaatga
     8161 ttatccttgc aggttctctc ttcggcattc agctgggtgc cattgggact acctatgtaa
     8221 agcccttcat gatcaaagtg gtgatgggca ttattatggt gatcgtgctc ttcagccgtg
     8281 ggttaatggt tcctgtctat atgtcacagc tagggttaat acacacatta ggcgaaacca
     8341 cggtgaaggc gttgaaaagc acaagtttcg caataatggc atttgccctg ttgctgggtg
     8401 cattcattgt tctgcgggcc atgtggcagg gacgtcgtgc agagcaggca gcaacgcaag
     8461 aagccctgga acacggcaaa atctaggaat ggctcggtac agaaagctcc ttgtggctgt
     8521 tgacggttcg gaatccagta cccatgcgct cagggagtct ttcaagcttg ccacaaacga
     8581 aagaagctgg atcacggttg tttctgtcgt ccctccttat gagggagaac tggaactcgt
     8641 gggtgtgaga gacgtcaaga cagcactgaa acagccctgc gagaaagccc tttcaatggc
     8701 gagtgatatc gcgaagaccg aaagggcctt aataaagacg gtgtgcgagg aaggggagat
     8761 ctacgagaga attgttgacc tcgcagatgc agaaaactgt gatttgatcg ttatggggag
     8821 aaaagggatg agccgcctcg aaaaaactct tgtcggcagt gtcaccgcaa gggttatcgg
     8881 tcacacccag agagacgtcc tcgtggtgcc ggacaattca cacattggct gggaaaggat
     8941 actcatctcc actgatggct cccgttacag cagggctgct gcgtcaaagg caatcgattt
     9001 cgcaatgtcc tatgggggtg aactgaggat cgtttcggtc gtggatgtcc caccagagtt
     9061 ttacgctgag gctcccaatg ctctggatga aatgattgcc agggcaaaag ggtataccaa
     9121 aaacctcaag attgaggcag agtcagcagg gataaaggca gaaaccttcg tgggagaggg
     9181 tgagccgtac aggtttataa gcgaccttgc cattaaacaa aaggtaaata ccatcatctt
     9241 gggctcgcac ggaaggacag ggttaaagag gctcctcatg ggaagtgtta ctgagaaggt
     9301 cattggccac gcgccatgtc ctgtgcttgt ggcgagacta taggagcgtc taaagaagga
     9361 ctagaagggg cgagtgaaga acatcccgcc ccttcttcat cattgagtct cgtttatatc
     9421 ccagagatat cgttacacca taataacaac cagccgtggc ggtcacatct gatcagcgct
     9481 tttgagagat agccaaactc tcctctcggc agcccgtccc ttttcaatac ggccttgact
     9541 ttatggatac caaaagttta tacttaaccc cgaggaaatt atgggcacgt acaccacata
     9601 ccatccgcac aagataaaga ggaaaaggac tcacggcttc cttgaaagga tgagctcgaa
     9661 aggtggccag aagactttgc agagaagacg atccaagggt cgcaagaggt tgtccgttta
     9721 agtccttgac catcagcaag ccagaacatc atatggccaa agtgattatc ctttttataa
     9781 aggcgtacca atacgtcttt tcacctcttt ttcacggaag ttgcaggttt gtcccgtcct
     9841 gttccgcttt ttccattgag gcactgggca aatacggagc gataaagggc atgtacttat
     9901 ccgttcggcg gatactcaga tgccatccct tccatcctgg cggctacgac ccggtacaat
     9961 aactatggat atggataaga gaacactcct tgcggtcgtc ctcgcactca tcgtctttat
    10021 cgggtcccag tattttttct cttcgccagc accaccgcct cctgcgcaac cacaaaaaac
    10081 agaaacaaag ccggaatcgt ccgataaagg aacgcctgct cctcaggtta ctcctctcgg
    10141 tcctgcaggt gccgaagaat ccggtccctc aaaggatata cgcattgaga cccaacttta
    10201 cgtcgcagtt ttttcttcac gggggggcat catcaagaaa ttcgacctga agacatataa
    10261 ggataaggac gggaatcccg tttctcttct gaaagcccct ggtgtgcttc ctcccctggg
    10321 acttggggaa aaggaagact tctcactgtc cagggttaat ttcgctgtac ggggaaccga
    10381 cctaatattg aggggttcag agacagggac tctca
//
LOCUS       DATKOU010000250         6571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2269690, whole genome shotgun sequence.
ACCESSION   DATKOU010000250 DATKOU010000000
VERSION     DATKOU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269690"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1275
                     /locus_tag="VMU21_11915"
     CDS             307..1275
                     /locus_tag="VMU21_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUO78270.1"
                     /translation="MIAFCMLRRTNSKDTGAEATIGRGEMPKGMTFVTIHKKGKYSLG
                     IKTGKGILDVKAASRLFRKKVPVIIDDVIQRGDQGLTGLVRTALKSKKAKRTFLDESS
                     IEFGPCVTNPEKILLLGFNYRTHAMETNTPVPSSPVLFTKYNNSLNSHNGVIRLPSHV
                     AYKFDYEVELVVVMGRSAHRVSEEEALSCVFGYCIGNDFSARDLQFKTSQFLLGKTCD
                     GFAPIGPYLATADQIPDPHTLAIECYVNGERRQSGNTSDMIFNVAATISYVSQHFTLK
                     PGDILFTGTPEGVIFGMPPEKQVWLKAGDKVTSVIEKLGTLTFTLA"
     gene            complement(1334..1804)
                     /locus_tag="VMU21_11920"
     CDS             complement(1334..1804)
                     /locus_tag="VMU21_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78271.1"
                     /translation="MQRFKLALDAWVRSVQEVMEYWPRTPKEFLERWMESTKNLQFLE
                     KGPEALDAWLKSQRDVLENWGKSQKDFLETWMESTKNFQQVFLDMANLREGSEASTLF
                     NSWFSTFSESCRTLTDEMKNIMDTWETTVEKQMETSGEMARQFFEIIRHERGKE"
     gene            complement(2059..2466)
                     /gene="gspG"
                     /locus_tag="VMU21_11925"
     CDS             complement(2059..2466)
                     /gene="gspG"
                     /locus_tag="VMU21_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HUO78272.1"
                     /translation="MSDRRGFTLVELLVVMVIIGLLATLVAPGLFKQLGKGQQSAAKA
                     QISSIEQALDKFRLDLGRYPTTQEGLSALVTNPGIEGWDGPYVKTGTITDPWKRPYQY
                     QQPGTHGEFDLFSYGRDGNPGGEGEDKDITSWE"
     gene            2566..3000
                     /locus_tag="VMU21_11930"
     CDS             2566..3000
                     /locus_tag="VMU21_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HUO78273.1"
                     /translation="MKYQIGKTGRVVVARFEDREDVLGNLVHIAKKENIRAAAFYLVG
                     GMREARIVVGPEKEEIPPTPVWRELGESHEVMGFGTIFYQGEEPKVHFHGAFGKKDMV
                     KVGCMREKTEAFLVLEAVMIELEGITAVREFDPVSGLTLLKL"
     gene            3240..3752
                     /locus_tag="VMU21_11935"
     CDS             3240..3752
                     /locus_tag="VMU21_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HUO78274.1"
                     /translation="MDILKAVKERRSIRDFQKKGIPQELLDKLIDALLWAPSAGNLQS
                     RKFYFIKDAYVKSEIASAALNQAFIAEASLVIVGCTDSRISSRYGDRGVTLYTIQDVA
                     VSIMGMMLVAHENGLGTVWVGAFREGDVAKILNTPRNLRPVAIIPVGYPSRIPSPPPR
                     VSKKEAIEFR"
     gene            4616..5881
                     /locus_tag="VMU21_11940"
     CDS             4616..5881
                     /locus_tag="VMU21_11940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUO78275.1"
                     /translation="MPYLAARVPPHWHVIHIDEEAEEIDGNIKADVVGITFHTPSAYH
                     AYTLAAHFRSRGICVVMGGPHVTLLPEEAGQYADVVFIGEAEGLWEEFLKGFEAGFYL
                     HAYQQAGVPSLENIPMARKALFHRRDHTGGVLFATRGCPNRCDFCTIAVMYRHGLRTR
                     PIAEVAAEYASFRGKIIIFWDENIAGDMAYAKELFRAIALYRKWWSSQASIQAGRDDE
                     FLEAAARSGCKQLFLGLESISQRSMAEVHKGFNRVEEYSNIIDRIHAHGIAVQAGIVF
                     GFDNDTPEIFKDTIDFLEETGIQNATFNILTPFPGTPLFLRMEAEGRIVTRDWRKYNS
                     RSEVVYQPKRMSAAELLAGFRYANERFYSLLSIAKRLSRSPVQIWWTLPLNLAYGYQW
                     RRVAQAAIQPSCAVDPALRSSSNGCPRPA"
     gene            complement(5949..>6571)
                     /locus_tag="VMU21_11945"
     CDS             complement(5949..>6571)
                     /locus_tag="VMU21_11945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUO78276.1"
                     /translation="GRGTLMNALASCCGKLAGIGGPLRPGVVHRLDRDTSGVMVVALD
                     DGAYYDLVEQFRKRTIHRKYVAMIYGNPRNDSGEITAPIGRSESDRKKMTTRTRRGKE
                     AITRWKVLKRFGMATLIEVRLGTGRTHQIRVHLSSIGYPVLGDRTYGRKTELEIKKRK
                     VFIPRQMLHAEMLGFMHPSSGKYMEFSGSMPEDMREVIDALSAGVQ"
BASE COUNT         1657 a         1585 c         1683 g         1646 t
ORIGIN      
        1 cggcggagga tctcaccatc gacgaattcc tgccctattt tataaaggag ccttaacgca
       61 gcaataagga gattcttacc cttttgcgag gggtctgcct tggactccca gagcgttttc
      121 ttgataaaaa cttctgctct actgaatatg aatgacatgg gctcccgcca tattatagcg
      181 cgcttcagtt aacctgtgtg ggcaaatatt tctatttctt gaagcggaag aggatgtcag
      241 cctaatgcca gcaaggttac atgcgcgttc tctgtttaag ggtattgttg acagggggaa
      301 cctttcgtga tagcattttg catgttgaga agaaccaatt ctaaagacac aggagcagag
      361 gctacgattg gccgtggtga gatgccaaaa ggcatgacct ttgtcaccat ccacaagaaa
      421 ggcaaataca gcctggggat taagaccgga aagggcattc tggatgtcaa agccgcgtcc
      481 aggttgttca gaaagaaggt gccggtcatc atcgatgacg tgatccagcg cggggaccag
      541 gggctgaccg gactggtgcg cacagcgctt aagagcaaga aggctaagag gacattcctg
      601 gatgaaagca gcatagagtt cggcccttgc gtaacaaacc cggaaaagat tctgctgctg
      661 ggcttcaact atcgtacaca cgccatggag accaacacac cggttcccag ctcaccggta
      721 ttgttcacca agtacaataa ttccctcaac agccataatg gcgtgatcag gctgcccagc
      781 catgtggcct acaagttcga ctatgaagtg gagttggtgg tggtcatggg caggagcgcc
      841 caccgggtga gcgaggagga agcgctgtcc tgcgtgttcg gttactgtat cggcaatgat
      901 ttcagcgccc gcgatctcca gttcaagacc agccagttcc tgcttggcaa aacctgtgac
      961 ggttttgcac ctatcggacc gtacctcgcg actgcagacc agatcccaga tcctcacaca
     1021 ctcgccattg agtgctacgt gaacggagag cggcgccagt ccggcaacac gagcgatatg
     1081 attttcaacg tcgctgccac catcagctac gtatcgcagc actttacact taagcccggc
     1141 gatatactct tcaccggcac gccggaaggg gtcatcttcg gtatgccgcc agaaaaacag
     1201 gtgtggctca aggccggcga caaggtcacc tcggtgatcg aaaagctcgg aacgctgacc
     1261 ttcacccttg cctgaggaag ggatcatatt ccttgacccg ctaggaactt tatgagaaga
     1321 taccgctcgt aacctattcc ttccctctct cgtgtcttat gatttcaaaa aattgtcttg
     1381 ccatttcccc gcttgtttcc atctgcttct caacagtagt ttcccacgtg tccataatgt
     1441 tcttcatctc atctgtcaaa gtcctgcaag actcactaaa ggtcgagaac caggagttaa
     1501 aaagagtgga agcctctgat ccttccctga ggttggccat atccaggaaa acctgctgaa
     1561 agttcttcgt ggattccatc catgtttcaa ggaaatcctt ttgggatttc ccccagtttt
     1621 ccaacacatc tctttgcgat tttagccatg catcaagagc ttccggccct ttttccagga
     1681 attgcagatt ttttgtggac tccatccacc tttccagaaa ctcttttggc gtcctcggcc
     1741 aatactccat aacctcctgg actgacctga cccatgcatc cagcgcgagt ttaaagcgtt
     1801 gcatacacgt cacctgcctc gttatttccc atacttaaat aattagcata aaaaggcctc
     1861 cctgtccact atcaaggtgt tctcaaggga aagatgcatt gtcataggat gcaggggatt
     1921 aaagggaatc agagggatga tgggaagcct atcgggaata tccttggcca ttcaccatgt
     1981 tccgtgctca tggacaagac ctgccaagta taacgcctta ggaagaattg atggtgagta
     2041 tgaggtgaag ttgcgggact attcccagct ggtgatgtcc ttgtcctcgc cctcgccgcc
     2101 gggattgccg tccctcccgt aggaaaagag gtcaaactcc ccatgggtgc cgggctgctg
     2161 gtattgatag ggtctcttcc agggatcagt gatcgttcct gttttcacgt agggcccgtc
     2221 ccagccttct atgccgggat ttgtgacaag ggcactcaga ccttcctgcg tggttgggta
     2281 tcttcccaga tccagccgga acttgtccaa tgcctgctcg atgctcgaga tctgcgcctt
     2341 ggcagcggac tgctgccctt tgcccagctg tttgaagagt ccaggtgcaa caagggttgc
     2401 gaggagaccg atgatcacca tgaccaccag cagttccaca agggtgaacc ccctgcggtc
     2461 cgacatgagt aatctcctcc acatcttggc cctcgtatat tgactcagga atttcattgc
     2521 tttaagctaa caataaatcg ctttataagt caatatggag gcattatgaa ataccagatc
     2581 ggaaagacag gaagggttgt ggtggcaagg tttgaagaca gggaggatgt gctcggaaac
     2641 cttgtccaca ttgccaaaaa ggagaatatc agggctgccg ccttttacct tgtgggaggt
     2701 atgcgtgaag cgaggattgt cgtcggtcct gaaaaggagg agatcccccc gacccctgta
     2761 tggagagagc ttggagaaag ccacgaggtg atggggtttg gaaccatatt ctatcagggt
     2821 gaagagccca aggtgcattt tcacggcgcc tttggcaaga aggatatggt gaaggtgggc
     2881 tgcatgaggg agaagacaga ggcattcctc gtccttgagg ctgtgatgat tgagcttgag
     2941 gggataacag cggtgaggga atttgatccg gtatcgggat tgaccctcct caaactctag
     3001 ccacagaaag tggtatgtgg gacaggtaga ggaaaagggt gatgctaggt tataagggcc
     3061 gtccgtttca ggatttttat aatcgacagg atattgatat ggcaaaccga gacgcattga
     3121 gtattaaggt tttgtccgga tctctcaata ggcctttctt acaaggtgtc gtataagaat
     3181 ccttcaccgc acggccctta tatttgtgcg gcaaggacat aaaagttcgg aggtgcacca
     3241 tggatattct caaggcagtg aaggagagaa ggagcataag ggattttcag aagaagggca
     3301 tcccccagga gcttctggac aaacttatcg atgcgttgct atgggcgccc agcgcaggga
     3361 acctccagtc ccggaagttc tatttcataa aagacgccta cgtgaagagt gaaatcgcct
     3421 cagcagctct gaatcaggcc tttatcgcag aagcatccct cgtgattgta ggatgtacag
     3481 acagcaggat ttcatccaga tacggtgacc gcggggtgac cctctacact attcaggatg
     3541 ttgcagtcag tatcatgggc atgatgctcg tggcacatga gaatgggcta ggcacagtgt
     3601 gggtgggtgc gttccgggaa ggggatgtgg caaagatact gaatacgccc cgtaacctca
     3661 ggcctgttgc cattatcccg gtgggttacc cgtcgagaat cccttctcca cctccgaggg
     3721 tgtcaaaaaa agaggcaatc gagttcagat aggtatttct cctacattga ttttttgcga
     3781 tctttctatt taataaacct ttagtatttg ctaaagttga gagagttttc agaatctacg
     3841 acagttagta ttaattgtta gacgtacaaa atgaatacta aactgaagac aattaagagg
     3901 cgactaaaac cgttgatctt aagtttgcct gaaatgatag accgatcatg gttgctgtca
     3961 gcatatctat cgataagaaa ggcatactcc ctgaactatc ataacgactc tttttcaaaa
     4021 ctttccaaag ccgaccaaga cgatatactg ctcaacataa actcgctaag ctctcttaag
     4081 accatgacac gtcacagaga atggattgcg ggttattatt ttaacaacgc catgtttaga
     4141 attgtggcat tagcagagac tggactaaaa gttctctata ccagagaaac taaaaagaag
     4201 gcgcctgagg acaattacgc atataataat ttgttattgc cttggtatga aggccgatac
     4261 gggattttgc ataatctcaa aaatgctcga aagcaagtca ataaatttaa gcatcgtaac
     4321 cgcgacagaa tgagaccaaa gacttcgaga ctcttagaga agctattgac gcttttaatg
     4381 aactactttc attattggaa aaaatataat ggcgtctaac aatcggctcg atagccgacc
     4441 ggaattaaag caagtttgat tttcgcaaag gtaattgccc cggcgcgtca gacttatcgt
     4501 tgacagaaat ggatccctat aacgagctat gccaacactt cttttaataa cagcctcttc
     4561 gcccgaaatt cggagagtga ggaggtcgcg ggtcctcaat tttcagcaga ttactatgcc
     4621 ttatctggcc gccagggttc ccccccattg gcatgtaatc cacattgatg aagaagcgga
     4681 ggaaattgac gggaacatta aagccgatgt ggtcggaatt acctttcata caccaagtgc
     4741 ctatcatgcc tatacccttg ctgcacattt caggtcgcgg ggaatatgtg tggtgatggg
     4801 tggtccgcac gtgacgctat tgcctgaaga ggccggtcaa tatgctgatg tggtattcat
     4861 cggagaggcc gaaggtttat gggaagaatt cctgaagggt tttgaagcgg ggttttatct
     4921 tcatgcgtat cagcaagccg gtgtaccgtc ccttgaaaat attcctatgg cccgcaaggc
     4981 gctatttcat aggcgcgacc ataccggcgg tgttcttttt gcgaccagag gatgccctaa
     5041 ccggtgcgac ttctgcacca ttgccgtgat gtaccgacat gggctacgca cacggcctat
     5101 tgctgaagtc gcggcagaat atgcttcttt cagggggaaa ataattatct tttgggatga
     5161 gaacatcgcc ggggatatgg cgtacgcgaa ggaattgttt cgcgccatcg cgctttaccg
     5221 gaagtggtgg agcagccagg cgagtattca ggcgggtcgg gacgatgaat ttttagaagc
     5281 tgcggcgcgc agcggatgca agcagttgtt tttggggttg gagtctatct cccaacgaag
     5341 catggctgaa gtgcataagg ggttcaaccg ggtggaagag tattccaata ttattgatcg
     5401 catccatgcg cacgggatag ctgtgcaagc agggattgtt tttggctttg ataacgacac
     5461 gccggagata tttaaagaca ccattgattt tttggaagaa accgggatcc agaatgctac
     5521 cttcaatatt ctcacaccat ttcctggcac accgctgttt cttaggatgg aagctgaagg
     5581 gcgtatagta acgagagact ggcgcaaata taatagccgc agcgaggtgg tctatcaacc
     5641 aaagcggatg agcgcggccg aactgttggc tgggtttcga tatgcgaatg agcgctttta
     5701 ttctcttcta agcatagcaa agcggctatc acgatcacca gttcagatat ggtggacatt
     5761 gcccttgaac ttggcgtatg gttatcaatg gcggagggtg gcacaggcag ccatccaacc
     5821 atcgtgtgca gttgaccctg cgcttcgctc ctcatccaac ggctgcccaa ggccagcgta
     5881 gtttctgtct cagaggtggc ttcaggcgaa gggcgcgccg ggtggctgaa acgggaccat
     5941 agcccctctc attgcacacc cgcgcttaat gcgtctatta cttctctcat atcctcaggc
     6001 atggagccgg agaactccat atatttacca gaagaaggat gcataaatcc aagcatctct
     6061 gcatggagca tttggcgcgg gataaagacc tttctcttct taatctccag ctcggtcttt
     6121 ctgccgtatg tcctgtcacc aagcacagga tagccgatag atgagaggtg gaccctgatc
     6181 tggtgcgtcc tccctgtccc cagtctcacc tctattaaag tggccatacc aaagcgcttg
     6241 aggaccttcc atctcgtgat cgcctctttc cccctccttg tcctggtggt cattttcttt
     6301 ctgtcggatt cagaacggcc aatgggagcc gtgatttctc ctgagtcatt cctgggattc
     6361 ccgtatatca ttgccacgta tttcctgtgg atcgtccgtt ttctgaactg ctccacgagg
     6421 tcataatatg ctccatcatc aagggcaacc accatgacac ccgaagtgtc cctatcgagt
     6481 ctgtgcacaa cgcccggtct caaaggcccc ccgatgccgg caagcttacc gcagcaggaa
     6541 gcaagggcat tcatgagagt cccccggccg t
//
LOCUS       DATKOU010000251         8537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3422610, whole genome shotgun sequence.
ACCESSION   DATKOU010000251 DATKOU010000000
VERSION     DATKOU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3422610"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..496
                     /locus_tag="VMU21_11950"
     CDS             137..496
                     /locus_tag="VMU21_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78277.1"
                     /translation="MNSRKNAARVEKLQRRVDAGFVATHFPEVASIVISMTYNQRGIA
                     KSLPRTVNFFPDSYALFRVDCLSKECVDGGFDLTRVITGMIRNHKVAAKGDLCCEGNG
                     SSADHSAIVYEVAIQYI"
     gene            671..1030
                     /locus_tag="VMU21_11955"
     CDS             671..1030
                     /locus_tag="VMU21_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HUO78278.1"
                     /translation="MPAERSRLVYSTDQQTIPLSGNHQRKKVSDKVLKATMPPAQQRV
                     TVRLDRKARCGKSVTVIEGLRMPQEDRVALLKQLKASLGTGGTVKDTSLEIQGDHRDA
                     LMAALENMGYSPKRSGG"
     gene            complement(1003..2040)
                     /gene="dusB"
                     /locus_tag="VMU21_11960"
     CDS             complement(1003..2040)
                     /gene="dusB"
                     /locus_tag="VMU21_11960"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HUO78279.1"
                     /translation="MLSIGHVTVPSSLVLAPMAGISDLPFRLINRSFGCELAFTEMIS
                     ASSLVHGSKNTLKMLSTTGNDRPLGVQILGADPESIKVALDVLSGYAFDLIDLNAACP
                     VKKVVSKGEGAALLREPLKLQKLLKFMVEKSGKPVTLKIRSGWDETQINAAEVALRAQ
                     DAGVSCLFIHGRTRMQGYAGEVDYRIIRTVKDFLRIPVIAGGDALTPHLIARLFQETG
                     CDGVTIARGALGNPWIFSETVEYLERGTVSYRPDVFDITRTMKEHLVLNAAFYGEKTG
                     VVLFRKFFTWYTRGIPVKGLKVSAFRAGTLNEMMQLIDEMQASLSDNYCCLTRRSEPV
                     PFSQPPERFGL"
     gene            complement(2120..2656)
                     /locus_tag="VMU21_11965"
     CDS             complement(2120..2656)
                     /locus_tag="VMU21_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78280.1"
                     /translation="MITFGQFSANFKGTDFKNEELKKFYGSVRKIIILRMAGKMSWSE
                     IYRLEETCQWPVLRRIGDPALSIYKGADKDLLKRFIETISEEKDLREKKSSLDKVKYW
                     SLLQSVIDERTSLFTQVFGFYKEDMEHCEMVAQRYSRIREKRIKTRKKMWKIGIGTGA
                     ATLTGAAALWYISKKDKK"
     gene            2758..3927
                     /locus_tag="VMU21_11970"
     CDS             2758..3927
                     /locus_tag="VMU21_11970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUO78281.1"
                     /translation="MLKKDVVIIGAGAAGLMCALTSGKRKRSVLLLEHTGKIANKVRV
                     SGGGRCNFTNLSVSSDHYASYNPHFCKSALARFTPHDFILLVEKHGIRYYEKEGGQLF
                     CKGSSGELIRMFQRECSDAGVEIATGCRIEAIKKEGCFIVTTNFGTVESSSLVIATGG
                     LSYPKLGATDSGMRIATQFGLKVTRLKPALVPLTLGKGDLKNLGELSGVSLDAIVSCN
                     KSPCGGKILFTHQGLSGPAILQSSLFWEKGNPVTIDLFPGKDPYALLAARRDSKMKIE
                     NLLSAYLPGRFARKWCEFSITPKVMNQYTERELRKIAYRLRNWQILPEGTEGYAKAEV
                     TLGGVDTEELSSRTMEAKKVPGLYFIGEVMDITGYLGGYNLQWAWSSGYAAGQHA"
     gene            4017..4637
                     /locus_tag="VMU21_11975"
     CDS             4017..4637
                     /locus_tag="VMU21_11975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HUO78282.1"
                     /translation="MKTIILASASPRRRDILKKTGLKFTVDVSRCEEKLNSGPKPRAL
                     ARLLSLEKAQSVAARHRNALILAADTFIFFKGEMLGKPHTNGEALRMLRRLNGKVHKV
                     ITGFTIIDTKSGKKISRSAETKVYFKRLTMKELESYAASGEPLDKAGGYAIQGLGAVL
                     IRKIEGDFFNVVGLPLDMVIQSLKEFGVNVPRIQDQLCHGSGTRKR"
     gene            complement(4657..5331)
                     /locus_tag="VMU21_11980"
     CDS             complement(4657..5331)
                     /locus_tag="VMU21_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HUO78283.1"
                     /translation="MFLKVGHRGASAYEIENTIESFSKALELGVNAVELDARQSKDGK
                     LILSHDETLKRLFGSDISMSQATLKELKQLTDNKILTLEEALRFLDSKVDKILVELKE
                     TGYEKKVLDVIMKEKLLNRTIIVSFHEATLAAVRSLHHKIETGLIYARLRNPVDAAKR
                     MGAGYLLPLYRFVHTRDVEKAHENNLKVIVWTINTKKEAREYRAKGVDGIASDKPDIF
                     EWMEQG"
     gene            5603..6154
                     /gene="msrB"
                     /locus_tag="VMU21_11985"
     CDS             5603..6154
                     /gene="msrB"
                     /locus_tag="VMU21_11985"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HUO78284.1"
                     /translation="MVRIIRKLLLAGVIMVMASFVVDAANLEKASTGGKWRTFVKPSK
                     EELKKRLSSMQYKVTQDNGTEPAFNNEYWNNKREGIYVDIVSGEPLFSSLDKYDSGTG
                     WPSFTKPLEPGNIAEKDDRSFFTRRTEVRSTHGNSHLGHVFTDGPKPTGLRYCMNSAA
                     LRFIPKEDLEKEGYGEFLKFFNK"
     gene            6229..6876
                     /locus_tag="VMU21_11990"
     CDS             6229..6876
                     /locus_tag="VMU21_11990"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HUO78285.1"
                     /translation="MSKEAVLHLHGFASSAYSTKAQYLGERFRKLPQIVFLVPDFNPT
                     PSDFKYLTITGMVNRLRQYILDHELGMFSMIGSSMGCLVALHYAQRFGGVKSLLLLAP
                     LLSYSSLPMNGEMLAKWEREGATEVFHYGFNERIPLRYDFHLDGLCYHEQIPPPVPVR
                     IIHGRNDETIPLEHSTKYAAEYPDMVAMKEVDSDHTLLDHLDFIWGQVAFILAST"
     gene            7094..7777
                     /locus_tag="VMU21_11995"
     CDS             7094..7777
                     /locus_tag="VMU21_11995"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78286.1"
                     /translation="MADKQKDTSKRTTPKLEIVLKCDSVGSMEAVAHALSELILPEVS
                     VTPIHQGLGAVSKSDVLLAETASRLIVGFQVDVLPGIGKVLKEHRVELRLYEVIYSLT
                     NDIKAIAEGMIPAVPQEQIIGSARIIALFKSTRKGIIIGCKILDGFLSLGQKFRIISA
                     IGPIYTSTIESLHVGQNVVQKATPGSEVGIKIQDFNKAHIGDLVESFRAVPLKVHPWE
                     PQGQIIRKE"
     gene            complement(7843..8238)
                     /locus_tag="VMU21_12000"
     CDS             complement(7843..8238)
                     /locus_tag="VMU21_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUO78287.1"
                     /translation="MPDLLIDHIGIVNKQEDEAVEFYQNLLGLEKIKESSVPPELSRQ
                     LFSLFQEIRMLVFVRGNLKIEVFLIPGFTPSSPNISHFCLQILHFPELVERLKQAGTK
                     VITGQHGGKTVYFAEDFSGNRIEIKPLSL"
     gene            complement(8251..>8537)
                     /locus_tag="VMU21_12005"
     CDS             complement(8251..>8537)
                     /locus_tag="VMU21_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78288.1"
                     /translation="KPNGETIVVRDPDHVSYPDPSQPYWIIVKDKNEIRTTGEVWVER
                     ALAGNEVVCQDSVVCTGIRCFHKFPHPKSEDCGKVCEGLDRAQSCRPYRI"
BASE COUNT         2163 a         2080 c         2145 g         2149 t
ORIGIN      
        1 gacaaggtca gctttgatac ggaaacgggc ccgaaaggtc ccaaggcaat caacgtcgtc
       61 aaactgtaat aagagggagg aaggcccctt ccggcggagg gggccttctt aatgagagga
      121 gaacatgaac aacagcatga atagcaggaa gaatgcagca agggtagaaa agctccagcg
      181 gagagttgat gcaggctttg tggctacaca cttccctgag gttgcaagca ttgttattag
      241 catgacctac aatcagaggg gaattgcgaa atcgttaccg cgcaccgtga attttttccc
      301 cgacagctat gcgttgttca gggtggactg cttgagcaag gaatgcgtcg acggcgggtt
      361 cgatctcacc cgggtaatca ccgggatgat cagaaaccat aaggtggctg caaaaggaga
      421 tctctgctgt gagggcaacg gctcttccgc cgaccactca gctatagtgt acgaagttgc
      481 catacaatat atataagggc attgccttct tatcatgcga tcagtttgga ggcccaacca
      541 gataccaccg agatacgtag gcgtaagaat tcccggtatg ccccaacgag ccacggaaac
      601 cgggattata aacacatcac aagcccgctg aacagcgcag ggataacccc tcccatgcta
      661 aaattaaccc atgccagccg aaagatcgag attagtctac tccactgacc agcagacgat
      721 ccctctgtca ggcaatcacc aacgaaagaa ggtctctgat aaggttctca aggcaaccat
      781 gcctcctgcg cagcagaggg taacggtccg gctcgaccgg aaagccaggt gcgggaaatc
      841 tgtcacggtc atcgagggac tccggatgcc tcaggaggat agggtagcgc tcctgaaaca
      901 attgaaagcc agtctcggta caggcggaac ggtcaaagat acttcccttg aaatccaggg
      961 cgaccaccgc gatgcgctta tggcagctct agaaaatatg ggctacagtc cgaaacgctc
     1021 cggcggctga gaaaagggaa ctggctcgct ccttctcgta agacagcaat aattgtctga
     1081 caacgaggcc tgcatttcgt cgatcaattg catcatctca tttagggtcc ctgcccggaa
     1141 ggccgatact ttcagtccct ttaccggtat cccccttgta taccatgtga agaatttccg
     1201 aaagagaacg acaccggtct tttccccgta gaatgcggca ttcaacacaa gatgctcttt
     1261 catggttctg gtaatgtcaa acacgtcggg tctgtaagat actgtccccc tctccagata
     1321 ctccactgtc tcagaaaata tccagggatt gccgagggcg cctcttgcta tagttacacc
     1381 gtcgcacccc gtctcctgga acagtctcgc gatgagatgc ggcgtcaagg catctccgcc
     1441 tgcgatcacc ggtattctca agaaatcttt taccgtcctt attatgcggt aatctacctc
     1501 tccagcgtat ccctgcatcc tcgtcctgcc gtgtataaaa aggcaactca cacctgcgtc
     1561 ttgcgcgcgg agggcgacct ctgctgcgtt gatctgtgtt tcgtcccagc cagagcgtat
     1621 cttgagcgtg acaggtttgc cactcttttc gaccatgaac ttcaggagtt tttggagttt
     1681 taagggttct ctgagcaggg cagcaccttc cccctttgac actaccttct ttaccggaca
     1741 tgctgcatta agatctatga ggtcaaatgc atagcccgag agaacatcca gtgccacctt
     1801 gatactttcc ggatcagcac cgagaatctg gactcccaaa ggcctgtcat taccggtggt
     1861 agaaagcatt ttcaaagtat tcttgcttcc atggacgagg gaactggcgc tgatcatctc
     1921 ggtgaaagcc agttcgcatc caaatgacct attgatcagg cgaaagggca gatcgcttat
     1981 cccggccatg ggggcaagga ctagggaaga aggaactgtc acatgtccaa tagagagcat
     2041 ggccatatat tatacatgag atgattggag aaaaaagaga gcggctgatg cgcggaacaa
     2101 ggctcctgga gcaagaatgt tacttcttat ccttcttcga aatataccag agcgcggctg
     2161 cgccggtgag tgttgccgcc ccggtcccta tgccgatctt ccacatcttc ttccgggttt
     2221 tgatcctttt ttcccttatc cttgagtatc tttgcgcaac catttcgcag tgctccatat
     2281 cctctttgta gaaaccgaac acctgagtga aaagacttgt tctctcatct atcactgact
     2341 ggagcaggga ccagtacttt accttgtcaa gagacgattt cttctccctt aagtcctttt
     2401 cttctgaaat ggtctctatg aaccgcttca gcagatcttt gtccgcaccc ttatagatgg
     2461 aaagcgccgg atcgccgatc ctccggagga ccggccattg acatgtctcc tccagcctgt
     2521 agatttcgga ccaggacatt ttccccgcca tcctgagaat gatgattttt cttaccgaac
     2581 catagaactt ttttaattct tcgttcttga aatcagtgcc cttgaagttc gccgaaaatt
     2641 gcccgaacgt tatcaccctc agtctcctcg catatgtgat tgactctatt gtaccatgcc
     2701 cgggggatgg gggaagacat ttttttgcgg gggtattggt ataattattc gggagagatg
     2761 ctgaagaaag acgtcgtaat tatcggggct ggcgcggccg gcctgatgtg tgcactcact
     2821 tcaggcaagc gcaagcggtc ggtgctgctt ctcgaacaca cagggaagat cgcgaacaag
     2881 gtgcgcgttt ccggaggggg gcgatgcaat ttcaccaatc tctctgtgag cagcgatcac
     2941 tatgccagct acaatcccca cttctgcaaa tcagcacttg cccgttttac cccacatgat
     3001 tttatccttt tggtggagaa gcacggtatc aggtattacg agaaggaagg gggtcaatta
     3061 ttctgcaagg ggagctccgg agagcttatt cgcatgtttc agagggagtg ttccgatgcc
     3121 ggggtggaga tcgccacggg ttgccgaatt gaagccataa agaaagaagg ctgtttcatt
     3181 gtcacaacga atttcgggac cgtggaatca tcctctctgg tcattgccac aggtggattg
     3241 tcttacccga agctcggagc gaccgatagc ggcatgcgaa ttgccacaca gttcggcctt
     3301 aaggttacca gactgaaacc cgccctcgtt ccccttactc ttggaaaggg agacctgaag
     3361 aaccttggag aattgagtgg tgtgtccctt gacgcaatag tcagctgcaa taaatccccc
     3421 tgtggaggca aaatcctctt cactcaccag ggactgagcg ggcctgccat cctccagagt
     3481 tccctttttt gggaaaaagg taatcccgtg accattgatc ttttccccgg caaggacccc
     3541 tatgcacttc ttgcggcaag acgtgactct aagatgaaaa tagaaaatct tctctctgca
     3601 tatcttcccg gacgttttgc aagaaaatgg tgcgagttct ccattacgcc aaaggtgatg
     3661 aatcaatata cggagaggga gttaaggaaa attgcataca ggctccggaa ctggcagata
     3721 ttacccgaag gaacagaggg atacgcaaag gcagaggtaa ccctcggagg tgtggatact
     3781 gaagaactct cgtccaggac tatggaggcg aaaaaggtac cgggcctcta ttttatcggc
     3841 gaggttatgg atattaccgg gtatttgggg ggatacaacc ttcaatgggc ctggtcatca
     3901 gggtatgcgg cgggtcaaca tgcataatga gcatctttcg acaagagcag ttttacagga
     3961 tacccttttc ttttgccggg agcggcgttt ctcaagggtc aaatgagaag ggcagtatga
     4021 aaacaattat tctcgcatcc gcatctccaa ggagacggga tattcttaag aaaacaggat
     4081 tgaagtttac cgtggatgtg agccgttgtg aagagaaatt gaactcagga ccgaaaccgc
     4141 gggccctcgc aaggctcctg tcccttgaga aagcacagtc ggttgccgcc aggcacagga
     4201 acgcccttat tcttgcagcg gacacattca tcttctttaa aggagaaatg ctcggcaaac
     4261 ctcacacgaa tggagaggcc ttgaggatgc tgaggagact gaacggtaag gtgcataagg
     4321 tgattacggg attcacgatt attgacacaa aaagcggaaa aaagatatcc cggtccgcgg
     4381 agacaaaggt ctactttaaa agactcacca tgaaagagct agaatcttac gcggcgtcag
     4441 gtgaaccttt ggataaggcg ggaggatacg cgattcaggg actgggtgcg gtccttatca
     4501 ggaagattga aggagatttt ttcaatgtgg taggtcttcc tctggatatg gtcattcaaa
     4561 gcctgaaaga attcggtgtg aatgttcccc ggatccaaga ccagctgtgc catggttcag
     4621 gaacgagaaa acgctaatgt atacagattc atcggcctag ccttgctcca tccattcaaa
     4681 gatatccggt ttgtcgctgg cgatgccgtc cactcctttg gcacggtatt cccttgcctc
     4741 tttttttgta ttgattgtcc acacgatcac cttcaggttg ttttcgtgcg ctttttcgac
     4801 atctcttgta tgaacgaatc tgtacagagg aaggaggtat cctgctccca tcctcttggc
     4861 cgcgtccacg gggttcctca atctcgcata gataaggcct gtttcgattt tgtgatggag
     4921 gcttcgcact gccgcaaggg tagcttcgtg gaaagagacg atgatggtcc tgttgagaag
     4981 tttttctttc atgattacgt ccaggacctt cttttcgtac cctgtctctt tcagttccac
     5041 cagaattttg tcgaccttgc tgtcgaggaa gcgcagggcc tcctccaggg taaggatttt
     5101 attgtctgtc aactgtttca gctcttttag ggtcgcctga gacatagata tatcacttcc
     5161 gaagagccgt ttcagagttt cgtcgtggct gaggatcagt tttccgtctt tagattgccg
     5221 cgcatccagt tcaacggcat tgacacccag ttcgagcgcc ttactgaaac tctcgatggt
     5281 attttcgatc tcgtacgcac ttgctccgcg atgccccact ttcaggaaca tgaaggtttc
     5341 ctttgccggt acggcttatt ccttgtcttc attcaatacc tccgagtcga gtttttttga
     5401 acttcatgag taaaaactgc aaagccgaat actttgcaat ccagacggca acatacgcct
     5461 tcggctctgc tctcaatata gatgaatcac gccccaggaa tcaactttaa ttcttcccgg
     5521 tgtgaaggag tcttcacatt tttttcatca tttcgatgta ttcttaagct aaaagggaag
     5581 ttgggtgcaa ggagaggcag ctttggtacg tattatcagg aaattgcttt tggcaggagt
     5641 gataatggta atggcatctt ttgtcgtgga cgcagcaaat ctcgaaaagg cgagcaccgg
     5701 ggggaaatgg aggacatttg tgaaaccatc aaaggaagaa ctgaagaaga gactcagctc
     5761 tatgcaatac aaggtgaccc aggacaacgg cacagagcca gcctttaata acgaatattg
     5821 gaacaacaaa agggagggga tctatgtgga cattgtttcc ggagagcccc tcttcagctc
     5881 tctggacaaa tacgactcag gcactggttg gccgagcttt acgaagccac tggaaccggg
     5941 caatattgcc gagaaggacg atcggagttt ttttacgagg aggaccgagg tgaggagcac
     6001 gcacggtaat tcccatcttg ggcatgtctt tactgacggg ccgaaaccca caggtctccg
     6061 ttattgtatg aactccgctg ccctcaggtt tatccccaag gaggaccttg aaaaagaggg
     6121 ctacggagag ttcctgaagt tcttcaacaa gtagcatgtg catgatttcc atacctgata
     6181 taatctaggt atcttatcta ctactcattg acaggagagg gcaagaaggt gtccaaagaa
     6241 gcagtgctgc atctccacgg gtttgcgtct tctgcatact caacaaaggc acagtatctg
     6301 ggagaacggt tcaggaaact ccctcagatc gtgttccttg tcccggactt caaccccaca
     6361 cccagtgact tcaaatattt gaccatcacc ggtatggtca accgcctcag acagtatatc
     6421 ctggaccatg agcttggcat gttcagtatg atagggagct ccatgggctg tctggtggcc
     6481 ctccactatg cgcaacggtt tggcggagtg aagagcctcc ttctcctcgc gccgctcctt
     6541 tcttacagtt cactgcccat gaatggagag atgctcgcga aatgggaaag ggaaggggca
     6601 acagaggtgt tccattacgg gttcaatgag aggattccct tgagatacga ttttcacctc
     6661 gacgggctct gttaccatga gcaaattcct cctcctgttc cagtacgcat catccacgga
     6721 aggaacgacg aaaccatacc attggaacac agcacgaaat atgctgcgga atacccggac
     6781 atggtggcaa tgaaagaggt tgattccgat catacgctcc ttgaccatct tgacttcatc
     6841 tggggacagg tcgcatttat tctggcatct acctaagatc taggaataca tggcataaaa
     6901 agttttttct tcgcagataa tttcctggat ctttaccttt tttgaaccta tccctccccg
     6961 gcgcattgct ttatattgaa aatcgtgtct tggggcatgc gtggagggtt aatttgagat
     7021 tccctcagtt acgtggtagc atttaaatgt gatccattca tgtttttgac gtaattgcca
     7081 aagggagcga cgcatggcag ataaacagaa agatacttct aagaggacaa caccgaagct
     7141 cgaaatagtc cttaaatgcg actctgtagg gagcatggaa gcggtggccc atgcgctttc
     7201 ggaactgatt ctccccgagg tttcggtcac tcctattcac cagggactgg gtgcggtcag
     7261 caaatctgat gtgttactgg cagagacagc aagcagactc atcgtcggtt ttcaggtgga
     7321 tgtgctgcca ggaatcggta aggtactcaa agagcaccgc gttgaactgc ggctctatga
     7381 ggtaatttat tcactgacca atgatatcaa ggccatagca gaaggcatga tccctgctgt
     7441 gccacaggag caaattatcg gctctgccag gataatcgcc ttgtttaaga gcacgcgaaa
     7501 agggatcatt ataggatgca agatcttgga cggttttttg tccctcggtc aaaagttccg
     7561 gattatttct gccattggcc ccatttatac gagtactata gagtcacttc atgttggaca
     7621 aaatgttgta cagaaggcaa ctccgggatc agaagtgggg ataaaaatac aggacttcaa
     7681 taaggcacac atcggggacc ttgtggaaag cttccgtgcc gttcccctga aggtccaccc
     7741 ctgggagccc caggggcaaa tcattcgaaa ggaataagaa aaataaagca aaagacgggt
     7801 taggaaatcc gcatagataa cccgttcttt ttgccctgat gcttataaag aaagaggttt
     7861 tatttcgatg cggtttcctg agaaatcttc cgcaaaatag acggttttgc cgccatgctg
     7921 tcccgtaatc actttggttc ccgcctgttt tagtctttcg acaagctccg ggaaatgaag
     7981 aatctggagg caaaaatggg aaatattcgg agatgagggg gtaaagccgg ggatgagaaa
     8041 tacctcgatt ttcagattcc ccctgacgaa cacaagcatt cttatctcct gaaaaaggct
     8101 gaacaactga cgggaaagtt cgggaggtac cgaggattcc tttatctttt ccagtcccag
     8161 aaggttctgg taaaactcca cagcctcatc ttcctgttta ttgacgatgc cgatatggtc
     8221 tatcagaagg tcaggcaatg tcggtacctc tcatatcctg taaggcctgc agctctgggc
     8281 cctgtcaagc ccttcgcaaa ccttgccgca atcctctgat ttcggatggg gaaatttgtg
     8341 aaaacacctt atgcctgtgc agaccacaga gtcctggcag acaacctcat ttcctgccag
     8401 tgccctttct acccagacct ctccggtggt ccggatttcg tttttgtcct tgacgatgat
     8461 ccagtagggt tgtgagggat caggataact gacgtggtcc ggatcacgga ccacgatggt
     8521 ctcgccgttc ggttttt
//
LOCUS       DATKOU010000252        21136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3423055, whole genome shotgun sequence.
ACCESSION   DATKOU010000252 DATKOU010000000
VERSION     DATKOU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 21136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21136
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3423055"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..496
                     /locus_tag="VMU21_12010"
     CDS             125..496
                     /locus_tag="VMU21_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78289.1"
                     /translation="MNNSMNSRKNAARVERQQRKLDAGFMVAQFPEVASIVISMIYNQ
                     RGIQKSLPRVVNFFPSSYALFRVDCLSKECVDGGFDLTQVITGMVRNRKEAAKGDLSC
                     EGDGPSADHSTIVYEVAIQYA"
     gene            620..818
                     /locus_tag="VMU21_12015"
                     /pseudo
     CDS             620..818
                     /locus_tag="VMU21_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            871..1248
                     /locus_tag="VMU21_12020"
     CDS             871..1248
                     /locus_tag="VMU21_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78290.1"
                     /translation="MTYREKRDGRIEKMQQKHDAGLISKRFPEVSSIVVDMEHRRSGI
                     SAILILRTLHFSSGSDAYFHVECLNRDCKDCAEGFDLDQVVGAMVRSHASSREGELDC
                     DGNGLTSRHVNISYKVTIQYNEN"
     gene            1522..2805
                     /locus_tag="VMU21_12025"
     CDS             1522..2805
                     /locus_tag="VMU21_12025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUO78291.1"
                     /translation="MSFISFNLHPQIMAGVKALGYDTPTPIQRQAIPPVLRGHDVMGL
                     AQTGTGKTAAFVLPILERLMKGPRSSVRALIIAPTRELAEQIHKAIGSMGKQTGLRSA
                     AVYGGVSANLQIQKLRAGAEIVVACPGRLLDHINQGTIKLSRMEVLVLDEADRMFDMG
                     FLPDIRKIIKHVPVKRQTLMFSATMPDDIQRLVREVLHAPVTVQVDFSVPADTVAHAL
                     YPVDQHLKTALLLELLHHTDTESVLIFTRTKHRAKRVGQQLEKAGHRAVSLQGNLSQN
                     RRQAALDGFRDGSYRILVATDIAARGIDVSSISHVINYDMPGTADAYTHRIGRTGRAA
                     KTGDAFTFIGYEDEDMVRSIERVLGEKVERRTLEGFDYKKSAPARDTEFARPPRKPQR
                     DQQQRTSGGPLKTRQGASPSAAVHRSFRSSVPSSS"
     gene            3360..4328
                     /locus_tag="VMU21_12030"
     CDS             3360..4328
                     /locus_tag="VMU21_12030"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78292.1"
                     /translation="MKLGEALVKEGLITRQQLELALQRQVQFGGRIGTNLVELQILGE
                     EELTKFLSSYFRIPAVTPEMIASIDEEVMKSLEKEIIEKYKILPFRKERNRLHTAMLN
                     PNNLQEIDELRFVTGFDIIPYVIAELRLLYSLEKLFGIKRELRYISLTDRFNPEAKVE
                     EESIDKVKVTFTEVKETEEIAGILIQAAYKIASRVAVFIMKGGKIVGWKGRGINVDGF
                     AVNEKESREEGLSLFSDVLRRKAYYRGPVLNIKGNEPLIKILSGTPQDALLIPIEIRD
                     KVIALLYIDNGTTSVLNANVGYLSKLATMAGIAFEIIILKKRILEQ"
     gene            complement(4334..4921)
                     /locus_tag="VMU21_12035"
     CDS             complement(4334..4921)
                     /locus_tag="VMU21_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO78293.1"
                     /translation="MTIGLCGSHRTGKTTLAEDISQRTGIPFVKTSTSEVFKEHGLDP
                     SKPMEFSKRLWIQHKILDAAEKAWNSSHGQFITDRTPLDMAAYTLADIQGSTEVDFGE
                     LEGYLGRCIRVTNAFSKLLVLVQPGIPLVYETGKAALNEGYLEHLNFLILGLCNDDRI
                     KGTLLYLPRDITDREARVKKVVEVLDSLKAGIRPR"
     gene            complement(5193..5687)
                     /gene="nrdR"
                     /locus_tag="VMU21_12040"
     CDS             complement(5193..5687)
                     /gene="nrdR"
                     /locus_tag="VMU21_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HUO78294.1"
                     /translation="MKCPFCGNLEDRVMDSRTSKEGESIRRRRECLKCGKRFTSYERV
                     EDIIPMVVKKDGRRETFDRAKILHGLKKACEKRPISIDVLDRVADSIEKKLTGLGVKE
                     IPSAWIGEEVMSSLKETDKVAYVRFASVYRQFRDLNDLMDEVKTLFEHQGPHAQKKAE
                     GKSS"
     gene            complement(5687..6940)
                     /gene="glyA"
                     /locus_tag="VMU21_12045"
     CDS             complement(5687..6940)
                     /gene="glyA"
                     /locus_tag="VMU21_12045"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUO78295.1"
                     /translation="MVVRDIELEQLRRVDPDVYQALEKEKSREREKIVLIASENYASQ
                     AVLEAQGSIFTNKYAEGYPGRRYYGGCEYADEVETLAITRAQELFGAEHVNVQPHSGS
                     QANMAVYLAFLKPGDTILGMSLSHGGHLSHGASVNFSGHLFRSVTYGVDSKTGYIDYN
                     ELRRIAREHKPRMIIAGASAYSRILDFQAFSDISKEVGAFFMADIAHIAGLIAAGLHP
                     SPIPYADFVTTTTHKTLRGPRGGMIMCKSAHAKAIDKMIFPGIQGGPLVHVIAAKAVA
                     LKEALSKEFRTYQQQVVRNAQKLAQGLSARGFKIFSGGTDNHLMLVDLTGENITGKEA
                     EEALDRTGITVNKNAIPYDSRPPAVTSGIRLGTPCVTTRGMAESEMTEIADIISHVLR
                     DNNNQDAMSLLSQRGKTLCTRFPIY"
     gene            complement(6927..7382)
                     /gene="rpiB"
                     /locus_tag="VMU21_12050"
     CDS             complement(6927..7382)
                     /gene="rpiB"
                     /locus_tag="VMU21_12050"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HUO78296.1"
                     /translation="MKIGIGSDHAGFELKQEIISLLKEMGVECVDYGTGGPHSVDYPD
                     FGEMVSREVSRGRIERGILICGTGIGMSIVANKFPGIRASLCNDLFTAKMSRLHNDAN
                     ILVIGGRIVGKDLAKEIVRVWFSTAFEAGRHTKRLDKIVQIEGRIHGSA"
     gene            7490..10042
                     /locus_tag="VMU21_12055"
     CDS             7490..10042
                     /locus_tag="VMU21_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HUO78297.1"
                     /translation="MDFCRFERPSRYINRELNALYKSAEVKVALAFPDIYDVGMSHLG
                     LKILYQVINDIPYASAERVFAPWLDMEAEMKARGIPLASLETRRQLKDFDIVGMSLQY
                     ELSYTTVLNMLSLAKIPLRAEAREGRDPLIIAGGPCTINPMPMSPFMDAFLVGDGEDA
                     VKDIVETYYLWKREGDGKKESLFLGLSRIPGLYVPSIHGNKGGFRVARRYKECLDDAP
                     YPVSPVVPYAPIVHDRITIEVSRGCTMGCRFCQAGMIYRPLRERSPETVLRIAEDSLK
                     NTGYEEISLTSLSAGDYSALLPLVRKMNRKFSQKVVSLSLPSLRVAAVNREVLREIKM
                     VRKTGFTMAPEVATERLRTVINKDFGEEDYERALHALFAEGWENLKLYFMIGLPTERD
                     EDIEAIPEMALKAIRIAKRYSRRYVNLNVGISPFVPKPHTPMQWEGQEAMEKIKTKMN
                     YLRQRLPKKGMNMKGHNPDMSLLEALFSRGDDGLSDLIEKAWSLGCRLDAWTEAFDFT
                     KWLDAAEYTGINVHAHAERNYGENDVLPWDGIESGVRKGFLWEELQKALSCEMTADCR
                     KVCHACGLGCADTFNAPSPGAGAPIHAIELLPELGKELPPAMRNRPLVPGKIRVRVRF
                     SKTGDLRYLSHRELMTAIIRAMRRADIPVAYSQGFHPSPRVSFGPPLNVGMSGKREYF
                     DMELRPGLGINDMKETLNRLLPEGLSIHDMGLIPEGEPSLQSFISRYEYEIICPDTKP
                     MEDFIAKGSFVVRREKTDRGNTDAVDLREMVEEAVIIDTKTMRVVVRDRGDKRVRLNE
                     LTAAIFQLSSGKLDITRLRLFGWRDGWVEPLPPLHEGASGKMEMEQPVSEFR"
     gene            10150..11655
                     /locus_tag="VMU21_12060"
     CDS             10150..11655
                     /locus_tag="VMU21_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HUO78298.1"
                     /translation="MPSEILINVTREERRVGLLEGGQVSEFYVERKRDASLVGNIYKG
                     KVVKILPGMQSAFIDIGLEKAAFLYVADIISDMEDYAPFLEEEDKSNSIELVSRRARQ
                     DLPIEELIQDGQELLVQVSKDPMGSKGARVTSYVTLPGRYLVLMPNLEHIGISRRISD
                     EEERARLRGIVETIKPKGYGLIIRTASEGCTEEELKKDLEFLFLLWENIQKKKDRVVA
                     PCLLYSDLDLAFRSVRDLMSHDVERLVIDDEGEYERIKDFVKSYFPKLLSKIEIYTDR
                     EPIFEAFGVELDISRALGRKVWLKSGGYIVIDQTEAMTVIDVNTGKFVGKEDLEDTIL
                     KTNLEAVKEIAYQIRLRNLGGIIIIDFIDMEKMENREKVFSAFLEAMKRDRAKNTILH
                     ISELGLIQMTRKRVRESLGRILCEPCSYCEGKGFVKSPRSLCYEIFRKVTKLARHGGE
                     KIIVTAHPQVAELLSDEERTGLEDIENRYGVKVIVKEDAKLHQENYEVVEL"
     gene            11682..12485
                     /gene="larE"
                     /locus_tag="VMU21_12065"
     CDS             11682..12485
                     /gene="larE"
                     /locus_tag="VMU21_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HUO78299.1"
                     /translation="MATALNRLITILREMESAVIAYSGGVDSTFLLKAAALSGIRLMA
                     VTGASPTMPRQDFVDAEEMAKTLGIPHTIIETSELEKEAFRMNPPDRCFHCKSELFAK
                     LRCIAESEGYRHVLDGSNLDDLDDWRPGRRAALAHGVRSPLIEAGFKKQDIRDLSLEL
                     NLPTWDKPSSPCLSSRFPYGEPITAEALARVESAENVLRSFGFREFRVRHHGETARIE
                     ILEPDIPRFLDPNIRIPVTRHLRALGYRFVTLDLEGFRSGRLNEAVSGS"
     gene            complement(12512..14143)
                     /locus_tag="VMU21_12070"
     CDS             complement(12512..14143)
                     /locus_tag="VMU21_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HUO78300.1"
                     /translation="MVQGYENASSCWKEILSDVSRNRGLLSFIEKFALDNTSPSTIVF
                     GTSGWRGEIGTDYTFNNVRVVTSAIVRMFKESATPVMKSMGVSDFEEIKRRGIIVGHD
                     NRFLGPDFAREVIGILQKEGIRTWYGGEATTPEFSAAIETLHAACSINLTPSHNPANY
                     AGFKFNPSDGGPAGSEITTEIEEIANKMMDQRIVLPSILPLKTDKIDLTSLYIDYIKE
                     RKTVDLEKIHAFVTEGDCFICIDNVHGATRGRMQTILGNSERVKYLRTEDDFLFGGIA
                     PEPSEKNMAGVEKALGESRARFKLGAIMDPDGDRIRYTDGAMQVPMNYFGAMAFHFLH
                     TYKNIPGVVAKSVGTSNFVNAIAEKLGVAVKETKVGFKNFRPYLLRTSREKAVVAFEE
                     SDGISGFNHTLEKDALFGLLLAIEMMAVTGKNLSEYLDDIMDEFGHYYPDRSGIAVDR
                     SLVGEPLVQRLSAIQEQYRKGTSLTIGGIRRTVKDVITVDGTKLVFDDGSWLMIRPSG
                     TEPKVRFYIESRTEQGKKSVFETAERMTKEALGMV"
     gene            14351..15319
                     /gene="pdxA"
                     /locus_tag="VMU21_12075"
     CDS             14351..15319
                     /gene="pdxA"
                     /locus_tag="VMU21_12075"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HUO78301.1"
                     /translation="MKRLAVTIGDPGGIGPEVVLKALSSVADECLPIIMGDLSVIEEA
                     VRAFGITQFIKTISDAEDASPSPEILWCMDMKSLKSYARGKPAAENGKACVDYIRKAV
                     WLAMERKVDGIVTAPVSKEALKLAGFPWPGHTEMLAEFTGTKEYAMMLVGGPLKVILV
                     TIHTALKDVPALVTKERVLGTILLARKACAMLGIKEPKIAVAGLNPHAGESGVLGNEE
                     IKEIVPAIMRAKEQGIAVQGPYAPDVVFHKAYKGDVDIVVCMYHDQGLIPLKMIAFEK
                     GVNVTVGLPIIRTSPDHGTAYDIAWKGIADPSSMREAMRLALRLTV"
     gene            15374..16456
                     /locus_tag="VMU21_12080"
     CDS             15374..16456
                     /locus_tag="VMU21_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HUO78302.1"
                     /translation="MLFEPFSFSGLNFKNRLVRSATFEKRADEDGFVTEFLVELYEEL
                     TMGGIGLMITGNALVHPSGRSVPQMLCIHSDIYIHELRKLTTAVRGLGGLIALQLVHG
                     GRQSFPILLGGHDPIAPSAVYDTSTKIMPRAMTDTEIWEMIDAFADAARRGRIAGFDA
                     VQIHGAHGYLVSEFLSPHTNRRDDYWGGDEERRFHFLEEVYKAMRKEVGEDYPLMIKM
                     NADDLIEGGLKVEEAARAAMRLEELGIDAIEVSGGMYESDERTAKPDITSPDDEAYFR
                     DAGKVFKERLSVPVILVGGMRSRSVMEDVIEKGEADLISLARPLIREPDLPNKLKEGK
                     AKADCISCNGCMEFERFDVVKCVQLH"
     gene            16895..17791
                     /locus_tag="VMU21_12085"
     CDS             16895..17791
                     /locus_tag="VMU21_12085"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HUO78303.1"
                     /translation="MRSFKEIKHDYRFTEDDQKRLVALQPLMAEHTEEIISTLNLWFM
                     GTKGALTFFTSETLKSHVFKTQKAWFEELFSGTYENRFYEQLMKIGAAHVRRNVDVHY
                     LQRAANIIKNTCTGILSRGEQPVAEIASNIISVGKIIDISMNVIVSAYIEEEIRLYSP
                     VYKVKSALLTFSERFSQATNIMLVFALIGLTFGVGWLFLRDVLNIFSGDIEHGIISSL
                     GSMLLLWLMIELMNTEISHLKGGKFHISIFVGVALVTMIRETMIATLRHEKPESVYYL
                     IAAILVVGFVYWIVAKTEDRHK"
     gene            complement(17998..18300)
                     /locus_tag="VMU21_12090"
     CDS             complement(17998..18300)
                     /locus_tag="VMU21_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78304.1"
                     /translation="MKRKMYATVRRYEGVPDPANAAKQVDEKFVPFISSLPGFVEYYW
                     IDLRDGAMISITVFKTLADAIHANEQARVWVKENLSSVLPNPPRIEAGTIVAHKGS"
     gene            complement(18484..19731)
                     /locus_tag="VMU21_12095"
     CDS             complement(18484..19731)
                     /locus_tag="VMU21_12095"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUO78305.1"
                     /translation="MVYSYVETSTSKIPFHYGWVIVFTGTLCIFACLGFGRFALGMLL
                     PSMTSTLNLTYSQMGFISTANFFGYLTSVLISGFWAIRIRSRKLIFIALLLVGISMIL
                     VSQARSFRYVLVLYFLTGIGSGAANVPVMSLVSHWFASTIRGKAAGFIVIGSGFAIII
                     SGKLIPFINSLAGLEGWRISWLTLGSIVLVIAFIGLIFIRNTPEEKGLRPIGIDDTAP
                     FYSQIIDKMKMNIYKRGVLYHLGIIYFLFGFAYVIYATFIVTTLVQERGFSEAVAGNF
                     WSWVGFLSLFSGPVFGTLSDKLGRKAGLIIVFFLQMLAYILVATKLPGAFLYLSIGLY
                     GIVAWSIPSIMAAAVGDYVGAHKAGQAFGFVTFIFGFGQIIGPAMAGILAERTGSFSG
                     SFSMAAALAAIAIVLTAFLKKPR"
     gene            19840..20271
                     /locus_tag="VMU21_12100"
     CDS             19840..20271
                     /locus_tag="VMU21_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HUO78306.1"
                     /translation="MIIDEARDTFRDYVRKKGLRNTRQRDEILRAFLSEGKHITIDDL
                     YAIVKKENPEIGYATVHRNLKLMCECGLADEIKVGNKKTRYEQKLGHAHHDHLICLKC
                     GKFIEVKDEKIERLQDKLAEGKGFIPMRHKLEIYGVCKKCK"
     gene            20379..>21136
                     /locus_tag="VMU21_12105"
     CDS             20379..>21136
                     /locus_tag="VMU21_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HUO78307.1"
                     /translation="MFKRLFLSAVIVLGTAGAAFAAIPLITDDTGTQGRGNFQLELFG
                     EYGHDREGIITTKTSDVSATLTYGLIETVDIVLGIPYQAWSSDNSESLVKGNGIADLS
                     LEAKWRFYEKEGLSLALKPGFTIPTGDTEKGLGAGRPTYHLLFIATKQMKPWEFDMNV
                     GYLYNENTASERKNIWSASLDAQVEVVKDLKLALDVGLATNPDSSSDTPPAYVLGGLI
                     WSLRENLDIGLGVKVGLTKPETDISVRGGITWRF"
BASE COUNT         5276 a         5164 c         5674 g         5022 t
ORIGIN      
        1 gacaaggtca gctttgatac ggaaacgggc ccgaaaggtc ccaaggcaat caacgtcgtc
       61 aaactgtaat aagagggagg aaggcccctt ccggcggagg gggccttctt aatgagagga
      121 gaacatgaac aacagcatga acagcaggaa gaatgcagca agggtagaaa gacagcagcg
      181 gaagctcgac gcaggtttta tggttgccca attcccggag gttgcaagca tcgtcatcag
      241 catgatatac aatcagaggg ggatacagaa atcattaccc agggtcgtca atttctttcc
      301 gagcagttat gcgttgttca gggtggactg cttgagcaag gaatgcgtcg acggcgggtt
      361 cgatctcacc caggtcatta ccggaatggt cagaaaccgc aaagaggccg ccaagggaga
      421 tctcagttgc gagggcgacg gtccctcagc cgaccactcg acgattgtct acgaagttgc
      481 catacaatac gcgtagaagc gctgcttttt aatcggcaat caatggggag gagaaccagg
      541 cgtacgtatc ggcatttcct caatggctga aatgtaagtg tacttcggga tttcacccaa
      601 cacgaaagga gtgagcgcaa tgaccaaagg aaaagtaaaa tggttcaacg attccaaagg
      661 cttcggcttt attacgagag atgacggttc cgatgtcttt gtccaccatg cttcgatcca
      721 aagcaacggg ttcaaaactc ttgccgaagg cgatatggtg agctttgagg ttgaaagggg
      781 caaagggtcc gaaggcaatc aacgtcgtca aactgtaacc aacctgagaa gtccctctgc
      841 ggcgcagtgg cttcatctga aaggcgaata atgacatacc gcgaaaagcg tgatggaagg
      901 attgaaaaaa tgcagcagaa gcatgacgca ggccttattt ccaagcgctt tcctgaagtt
      961 tcgagcatag tcgttgacat ggaacaccgc aggagcggaa taagtgcgat ccttatttta
     1021 cgaactctcc atttttcgtc cggcagtgat gcgtatttcc atgtcgaatg cctgaataga
     1081 gattgcaaag attgtgcgga ggggtttgat ttggaccagg tcgttggtgc gatggtcagg
     1141 agtcatgcat catcgagaga gggcgagttg gattgtgacg ggaatggtct cacttccagg
     1201 catgtgaata tcagctataa ggtcactata cagtataatg aaaattaaaa aaacttacga
     1261 agtatccggc aatggcagga tcaggattta tccgtcattg tatgagaaaa aagaggggaa
     1321 taagttcgga tgttttctga gaaagttatt gcggttcatc cttcggtagc gaggccatgt
     1381 tctcaaaagc cctgccgctt ttcccagtct tagagaggca aaaagggttt ggctttctcc
     1441 gaataaagtc tatcctcccg tttctcaatt gccggctttc aggaaataag tcctgataac
     1501 ctattacaga aagaaggaca catgagtttt atttcgttca atctccatcc gcagatcatg
     1561 gccggcgtca aggcgctcgg ctatgacaca ccaaccccta tacagcggca ggccatcccg
     1621 cccgtcctcc ggggacacga tgttatgggc cttgcccaga ccggaacagg caaaacagca
     1681 gcctttgtgc tgccgattct ggagcggttg atgaagggac cacgcagtag cgtccgcgcc
     1741 ctcatcatag cccctacccg cgaactggca gagcagatcc acaaggcgat cggcagtatg
     1801 ggaaagcaga caggcctccg gagcgcagcc gtttacggag gcgtaagcgc gaatctgcag
     1861 atacaaaagt tgcgcgccgg ggccgagatc gttgttgcct gtcccggacg cctgctcgat
     1921 cacattaatc agggcacaat caagctttcc cgaatggaag tgcttgtgct ggatgaggca
     1981 gaccgcatgt tcgatatggg gttcctgcct gatatccgga agatcatcaa acacgtgccg
     2041 gtcaagcgtc aaacgctgat gttctccgca accatgcccg atgatatcca gcggctggtc
     2101 cgcgaagtcc ttcacgcacc ggtcacggtg caggtcgatt tctcggtacc ggccgatacg
     2161 gtggcgcatg ccctttatcc tgtggatcag cacctcaaaa ccgcgcttct tctcgaactc
     2221 ctgcatcata cagacaccga gtcggtcctt atcttcacgc gtaccaaaca ccgtgccaag
     2281 cgcgtggggc agcaactgga aaaggccggc caccgggccg tgtcgctgca gggaaacctg
     2341 tcgcagaaca ggcgacaggc ggcgctggac ggattccgtg atggctctta ccggatcctt
     2401 gttgcaaccg acatcgcggc ccgtggcatc gatgtttcga gcatatctca cgtcatcaac
     2461 tatgacatgc ccggcaccgc cgatgcatac acgcaccgga tcggccgcac cggccgcgcc
     2521 gcaaagaccg gcgatgcctt cacgttcatc ggttatgaag acgaggacat ggtgcgcagc
     2581 atcgaacgcg tcctcggtga gaaggtggaa cggcgtactc ttgagggctt cgactataag
     2641 aagtcggctc cagcgcgcga taccgagttt gcccgcccgc cgcgtaagcc gcagcgcgac
     2701 cagcagcaga gaacgtctgg cggaccgctt aagacacgac aaggcgccag tccgtcagca
     2761 gcggtccatc ggtccttccg ttcttctgtt ccctcttcgt cgtgacgtga aggaatgcct
     2821 ggagcttttg acggttcttc tctatcggtg cttttcgacc tgataaccgg tctggtagtt
     2881 gttttgggtc tcttcccata agcggctcca atcgtttagg agaggccctt gatttttttg
     2941 aagtgagcaa gtcgcccaga gtgtctccag gacatttccg gaggacatag ccgagaaaag
     3001 cgattatggt tggaatgtgc tttagctcct gctccgcccc gtgctcccaa ttccagaccg
     3061 tgttttcgga gacccctatc atgacagcaa cttcgatttg cagcagccct aagtcacggc
     3121 gtctgtttct gatgtggtct cccagggtct ggggtttcaa tgggtatcct ctaggtggta
     3181 taatcgggcg tgggattgca agtctatgtt ggatactcga aaaaggttac tcgacccctg
     3241 tccccgcgga tacctgagcg acccaaaaca ccagtgcacc tgcagcccca ggcagaccca
     3301 ccgttaccgg acaagggttt cgcgtcctct cctggacagg ggcgtgtttt aggaggatta
     3361 tgaaactggg tgaggcgttg gtgaaggagg gccttatcac gcggcagcaa ctggaactcg
     3421 ccctccagag gcaggtgcag ttcggcggca ggatcggcac caatctggtg gagctccaga
     3481 tcctggggga agaggagctc accaagttcc tgagcagcta tttcaggatt ccggccgtta
     3541 cccctgagat gattgcttcc attgacgagg aagtgatgaa atccctcgaa aaggagatta
     3601 tcgaaaaata caagatcctc cccttcagga aagagcggaa caggctccat actgccatgc
     3661 tcaatcccaa caatctgcag gagatcgatg agctgagatt cgtgactggg tttgacatca
     3721 tcccctatgt gattgcagaa ctgaggctcc tctattccct cgaaaagctt tttgggataa
     3781 agcgggagct gaggtatatc agcctgacgg accggttcaa tcccgaggcc aaggtggagg
     3841 aggagtccat cgacaaggtg aaggttacgt tcacagaggt gaaggagacc gaagagatcg
     3901 caggcatact gatacaggca gcgtataaga tcgcgtccag ggtcgcggtc tttatcatga
     3961 aagggggaaa gatcgtcggc tggaagggaa gggggatcaa tgtggatggc tttgcggtca
     4021 acgaaaagga gtcaagggag gaagggctct cgctattttc tgatgtgctg aggcgaaagg
     4081 cttactacag gggtccggtg ctgaatatca agggcaacga acccctgata aaaatacttt
     4141 ccggaacccc tcaggatgcg ctcctcatac ccattgagat cagggacaag gtaatcgccc
     4201 ttctttatat cgacaacggc accacctctg tgctcaatgc caatgtgggg tatctctcaa
     4261 agctcgccac catggcggga atcgccttcg aaatcattat tctgaaaaag aggatcctgg
     4321 agcagtagct cgttcagcgg gggcgtatac cggccttgag gctatccagt acctccacga
     4381 cctttttcac acgtgcttct ctatcggtga tgtcccgggg gaggtagaga agggtgccct
     4441 taatccggtc atcattgcag agaccgagga tgaggaagtt caggtgttca agatacccct
     4501 cattaagcgc tgcctttcct gtttcgtaca caaggggaat tcccggttgc accaggacca
     4561 gtagcttaga aaatgcgttg gtgaccctga tacaacggcc gagatatccc tccagttccc
     4621 caaaatcaac ctcggtggat ccctgaatat ctgcgagggt gtacgcagcc atatcaaggg
     4681 gggtcctgtc ggtaataaac tgcccatggc tgctgttcca ggctttttcc gcagcatcca
     4741 gtatcttatg ctgaatccag agcctcttgc tgaactccat gggtttggag gggtccaggc
     4801 cgtgctcctt gaagacctca gatgtgctgg tcttgacaaa ggggatcccg gttctttggg
     4861 agatatcctc agccaaagtg gtcttgccgg tcctgtggct tccgcataat cctatggtca
     4921 tggctattgc gggtagtgta atggttattt tcgaaaaagg gaagattagg ggcttactac
     4981 ccggtcttat gatatctaac ccacataatt catccgcttc tcacttactg agagagacgt
     5041 ctgtctctcc gtaagtgaat agccctttgt agcgaacggt ttcctagtga acctaagatc
     5101 agtctgttcc gagatatttc ctgaacaggc atctaacccg gactctcacg ttcgagccga
     5161 acaccccaat cagtaagtat gaataatttg ggctaagagg acttcccttc agcctttttc
     5221 tgggcgtggg gtccctggtg ttcaaagagg gttttcactt cgtccatgag gtcattaagg
     5281 tccctgaact gcctgtacac agaggcgaac ctcacatacg cgaccttgtc cgtctccttg
     5341 agggaagaca tcacttcctc tccgatccat gcgctgggta tttccttaac gcccagtccg
     5401 gtgagctttt tctctatgga atcggcgacg cggtccagga catcgatgct tatgggtctt
     5461 ttctcacatg cctttttcag gccatggagt attttggccc tatcaaaggt ctcccgcctg
     5521 ccgtcttttt ttaccaccat gggaatgata tcctccacac gctcatagga ggtgaacctc
     5581 ttcccgcact tcaggcactc tcgtctcctg cgtatggatt ctccttcctt gctcgtgcgg
     5641 gaatccatga ccctgtcttc caggttgccg cagaaaggac acttcatcag taaatgggaa
     5701 aacgcgtaca gagggttttc ccacgctggc tgaggagact catggcatcc tggttgttgt
     5761 tgtctctgag cacatgagaa atgatgtccg cgatttcggt catctccgat tcagccattc
     5821 ccctcgtggt gacacaggga gtcccgaggc gtatgccgct ggtgaccgca gggggcctcg
     5881 aatcataagg gatggcattc ttgtttacag tgatccctgt cctgtcaagg gcctcttcag
     5941 cctctttccc tgtaatattt tctccggtga gatcaaccag catgagatgg ttatcggttc
     6001 cgcccgaaaa gatcttaaat ccccttgcag acagcccctg cgcaagcttt tgtgcgtttc
     6061 ggaccacctg ctgctggtag gtcctgaatt ctttgctcag ggcctccttt aatgccaccg
     6121 cctttgcggc gatcacatgg accaggggtc cgccctgtat gccgggaaat atcatcttgt
     6181 cgatggcctt ggcgtgtgcg gatttgcaca taatcatgcc gccccttgga cccctgagtg
     6241 ttttgtgcgt ggtggtggtg acgaaatccg cgtaggggat gggcgaagga tgcagccctg
     6301 cagcaataag ccccgcgata tgggcgatat ccgccataaa aaaagccccg acctcttttg
     6361 agatatcaga gaatgcctgg aagtccagta tcctcgaata tgcactggca ccggcgatga
     6421 tcatcctggg cttatgttcc cgcgcgattc tcctcagttc attataatcg atatacccag
     6481 ttttactgtc aacgccatac gtgacgctcc tgaagagatg tcccgagaag ttcaccgaag
     6541 cgccgtggga aaggtgaccg ccgtggctga ggctcatgcc caggatagtg tccccgggtt
     6601 tcagaaacgc cagatagacc gccatatttg cctgggaccc cgaatggggc tgcacattca
     6661 catgttcggc gccaaagagc tcctgcgccc gagtgatggc gagggtctct acctcgtccg
     6721 cgtattcaca tccgccataa tatctcctgc cggggtatcc ttcagcgtac ttgttagtga
     6781 agattgagcc ctgtgcctcc aggaccgcct gactggcgta gttctcggaa gcgatgagga
     6841 caatcttttc tctctccctg ctcttctctt tctctagggc ttggtaaacg tcagggtcaa
     6901 cacgtctgag ctgttccagt tctatatcac gcactaccat gtatccgtcc ctctatctgc
     6961 acgatcttat ccagtctctt tgtatgccgc cctgcctcaa aggcagtcga aaaccatacc
     7021 cgcacaatct cctttgcaag gtctttcccc acaatccttc ccccgatcac caggatattt
     7081 gcatcgttgt gcaacctgct catctttgcg gtgaaaagat cattgcagag tgaggcccgt
     7141 atcccgggga acttgttcgc tacaatggac atccctatgc cggtaccgca gatgaggata
     7201 cccctctcga ttctccccct gctcacctcc cgggacacca tttccccaaa gtcagggtag
     7261 tccactgagt ggggaccacc ggttccgtag tcaacgcatt ccacgcccat ttctttgagc
     7321 agggaaatga tctcctgctt aagctcaaaa ccggcatggt cagatccaat tccaattttc
     7381 atcgcagccc tgagttctta tcttatccga gaggttttct ctaagtcaag aaaagcaggg
     7441 ggaagaggct ttattccagt attgttacgg cttatggtat tcttaaagta tggatttctg
     7501 ccgttttgaa aggcccagca gatatataaa ccgggagttg aacgcgcttt acaaaagcgc
     7561 ggaggtgaag gttgccctgg catttcctga tatctatgat gtgggcatgt cccatctcgg
     7621 tctgaagata ctctaccagg taatcaacga catcccctat gcctctgcag agagggtctt
     7681 cgccccatgg cttgatatgg aggcagagat gaaggcaagg ggaatccccc ttgcctcgct
     7741 cgagaccagg aggcaattga aggattttga tatcgtggga atgagcctcc agtacgaact
     7801 ctcttatacc acggtactga atatgctctc attggcaaag atccctttga gggctgaagc
     7861 cagggaaggc agggatcccc tgatcatcgc cgggggtccc tgcaccatta accccatgcc
     7921 catgtcaccc tttatggatg cctttctcgt cggagacggg gaagatgcgg taaaagatat
     7981 tgtggagacg tattatcttt ggaagaggga aggggatggg aagaaagagt cccttttcct
     8041 tggcttatca cgaatcccgg ggctgtatgt tccctccatc cacgggaaca agggtggatt
     8101 ccgggttgca cggcggtaca aggaatgcct ggatgatgca ccgtatcctg tttctccagt
     8161 ggttccttat gcccccatag tgcatgacag gataaccatc gaggtatcga ggggctgtac
     8221 catgggctgc aggttctgcc aggcagggat gatctacagg cctttgaggg agaggagccc
     8281 tgaaacggta ctccggattg ctgaggattc cctgaagaat acaggatacg aggagatatc
     8341 cctcacctcg ctcagcgcgg gtgactatag tgcccttctg cctctggtga gaaagatgaa
     8401 caggaagttc tcccagaagg tggtctctct ctcgctgcct tcgctgcgtg tagctgcggt
     8461 gaaccgggag gtcctgcgtg agataaaaat ggtgaggaaa accggattca ccatggcgcc
     8521 tgaggttgcc accgagaggc tcaggacagt catcaataag gacttcgggg aggaggatta
     8581 tgaaagggcg ttgcatgccc tttttgccga ggggtgggag aatcttaaac tctattttat
     8641 gatcggcctg cctactgaaa gggatgagga tattgaggca atccccgaaa tggcattgaa
     8701 ggccataaga attgcaaaga ggtattcccg cagatatgtc aacctgaacg tggggatatc
     8761 accctttgtg ccgaaacccc atacacccat gcagtgggaa gggcaggagg cgatggagaa
     8821 gataaagaca aagatgaatt acctgcgcca gaggctcccg aagaagggga tgaacatgaa
     8881 gggccataac cctgacatga gtctcctcga ggctcttttc tcgagggggg atgacggatt
     8941 gtccgatctg atagaaaagg catggtccct gggctgcagg cttgatgcat ggacagaggc
     9001 atttgatttt acaaaatggc ttgatgccgc agaatatacg gggatcaatg tccatgctca
     9061 cgcggaaagg aactatggtg aaaacgatgt ccttccttgg gacgggatag agagcggggt
     9121 caggaaaggg tttctctggg aggagttgca gaaggccctc tcctgtgaga tgacggccga
     9181 ctgcaggaag gtgtgccatg cctgcggact ggggtgcgcc gatacattca atgccccttc
     9241 acctggggca ggagccccaa tccacgccat cgagcttctg ccggagcttg gaaaagaatt
     9301 gccccctgca atgcggaatc gaccgcttgt ccctggtaaa atcagggtcc gggtccggtt
     9361 ttcgaagacg ggggatttac gctacctttc ccacagggaa ctgatgaccg ccattattcg
     9421 tgcgatgaga agggcggata tcccggttgc gtattctcag ggttttcacc cttcgcccag
     9481 ggtatccttt ggcccgcccc tcaatgtggg tatgagcgga aagagggagt actttgatat
     9541 ggagctgagg ccgggccttg ggattaatga tatgaaggaa acccttaacc gcctgctccc
     9601 tgaaggactg agcattcacg atatgggact tataccagag ggggagcctt ccctccagag
     9661 cttcatatcg aggtatgaat atgagataat atgccctgat acgaagccaa tggaggattt
     9721 tattgcgaag gggtcctttg tggtccggag ggaaaagacg gacagaggca atactgatgc
     9781 cgtagatttg agggagatgg tggaagaggc ggtcattatt gataccaaaa ccatgagagt
     9841 tgtggttagg gacagaggag acaaaagggt gaggctgaac gaactcactg ccgcgatttt
     9901 tcagttgagt tccggaaagc ttgatattac aaggctccgt ctttttggtt ggcgtgatgg
     9961 atgggtcgaa cccttgcccc ccttgcatga gggagcatcc gggaaaatgg agatggaaca
    10021 acctgtctca gaattccggt aatccctggt gtattgacag ggagcctgtg tcttcagggt
    10081 cccatatacc gggattttca ttctcttatt ggttacgaaa atatatcact ctatagaggg
    10141 agaagaagaa tgcccagcga gatacttatt aatgtgaccc gtgaggagag aagggtgggg
    10201 ctgctggaag gaggccaggt cagtgagttt tatgtggaaa ggaagaggga tgcgagcctt
    10261 gtgggaaata tctataaagg aaaagtagtg aagatactcc cgggaatgca atccgccttt
    10321 atcgacatag gcctggaaaa ggcagcattc ctctatgttg cggacatcat aagcgatatg
    10381 gaggactatg cgccctttct cgaggaggag gataagagca attccataga gctcgtttcc
    10441 aggagggcac ggcaggacct tcccatcgag gagcttatcc aggacggtca ggaattgctg
    10501 gtgcaggtct ccaaggaccc gatggggagc aaaggggcac gggtgacgtc ctatgtaacc
    10561 cttccaggac gctatctcgt gctcatgccg aaccttgagc atatcggcat ctccaggcga
    10621 atatctgatg aggaggagcg ggcaaggttg cggggaattg tcgaaaccat caagccgaaa
    10681 gggtatgggc ttatcataag aaccgccagc gaggggtgta ccgaagagga gctcaagaaa
    10741 gaccttgagt ttttgttctt actgtgggag aacatccaga aaaagaagga cagggtagtg
    10801 gctccgtgcc tcctttacag cgatcttgac ctcgcattca gaagcgttcg tgaccttatg
    10861 agccatgatg tggagaggct ggtgatagat gacgaggggg aatacgaaag gatcaaggat
    10921 tttgtgaaga gctatttccc gaagctcctt tccaagatag agatctatac cgacagggaa
    10981 cccatctttg aagcctttgg ggtggagttg gacatatcca gggcgctggg aagaaaggtg
    11041 tggctcaagt ccggcggcta tattgtgatc gaccagacag aggccatgac cgtgattgat
    11101 gtgaacactg gaaagtttgt gggcaaagag gacctggagg ataccattct caagacgaat
    11161 ctggaggcgg tgaaagagat cgcgtatcag atcagactga gaaacctggg agggatcatc
    11221 atcatagact tcatagatat ggaaaagatg gagaacaggg aaaaggtctt cagtgcattt
    11281 ctggaggcga tgaaaagaga tagggcaaag aacaccatac tccatatttc ggagctgggg
    11341 ctcatccaga tgacgagaaa aagggtgagg gagagcctgg gacggatact gtgcgagcca
    11401 tgttcctatt gtgaaggaaa agggttcgta aaatccccca gaagcctctg ctatgagatc
    11461 ttcaggaagg ttaccaaact ggcgaggcat gggggggaga agatcattgt gaccgcgcat
    11521 ccccaagtgg cagaactcct ctcggacgag gagaggacgg ggctggaaga cattgaaaac
    11581 aggtacgggg tgaaggttat cgtgaaggag gatgccaaac tccaccagga aaactatgag
    11641 gtggtggaac tatagaaccg gcactcggca gcaatgactc catggcaacg gcactgaatc
    11701 gcctcattac tattctcaga gagatggaga gcgctgtcat tgcctattcc ggaggtgtgg
    11761 acagcacctt tcttcttaag gctgcggcat tgtccggcat acgattgatg gcagtgaccg
    11821 gcgcctctcc cactatgccc cggcaggatt tcgtggatgc agaggagatg gccaaaaccc
    11881 tgggaatccc gcacaccatc atcgaaactt cggaactgga gaaggaggct ttccggatga
    11941 atcctcctga ccggtgcttc cattgcaaga gtgaactctt cgcaaagctg agatgtattg
    12001 cagaatccga ggggtaccgg catgtccttg atggaagcaa tctggatgac cttgacgact
    12061 ggagacccgg caggagggca gccctggccc acggggtgag gagccctctc atcgaggcag
    12121 gatttaaaaa acaggacata cgagacctct cccttgaact gaatcttccc acatgggaca
    12181 aaccttcttc cccatgcctt tcgtcgagat ttccctatgg ggaacctatc accgcagagg
    12241 cgctcgcaag ggtggagtca gcagagaatg tcctgaggtc ctttggtttc agagaattca
    12301 gggtgagaca ccatggagag actgcgcgga tagagatcct ggagcctgat attcccagat
    12361 tccttgaccc caatatacgc attcctgtga caaggcacct cagggcactc ggatacagat
    12421 ttgttacctt ggatcttgag ggcttcagaa gcggaagatt gaacgaagcg gtctccggtt
    12481 cctaatcttt tcagcattaa ccccttctca ctcataccat ccctaatgcc tcttttgtca
    12541 ttctctcagc cgtctcgaag acagacttct tgccctgctc tgtccttgac tctatgtaga
    12601 acctcacctt cggctctgta ccggaaggcc ttatcatgag ccatgagccg tcatcgaaca
    12661 cgagcttcgt gccatctacg gtaatgacgt ccttcacagt cctccggatt cctccaatag
    12721 taagagacgt tcccttcctg tactgttcct gtatcgcaga gagcctctgc accagaggtt
    12781 cccccaccag agagcggtca accgcgatgc cggaacggtc tgggtaatag tgtccaaatt
    12841 catccataat gtcatcgaga tattcgctca ggttcttgcc ggtgaccgcc atcatctcaa
    12901 tggcgagaag gagtccgaag agggcatctt tttcgagcgt gtggttaaat cctgatatgc
    12961 cgtcagattc ttcaaaggca acaaccgcct tttctctgga tgtcctgaga agataaggcc
    13021 tgaagttctt gaacccaacc tttgtttcct ttaccgccac tcccagtttt tccgcgatgg
    13081 cattcacaaa gttggatgtc cccaccgact ttgccaccac ccctggaata ttcttatacg
    13141 tgtggaggaa atggaacgcc attgccccga aatagttcat gggtacctgc atggcaccgt
    13201 cggtgtaccg tatacggtca ccgtccgggt ccatgatcgc gccaagtttg aatctggccc
    13261 tgctctcccc cagggccttt tcgacccctg ccatgttctt ttccgaaggc tctggcgcaa
    13321 tgccgccaaa gaggaaatca tcctctgtcc tgagatattt gaccctctcg ctatttccca
    13381 gtatagtctg catccgtccc cttgttgcgc catgcacatt atcgatacag atgaagcaat
    13441 ccccttctgt gacgaatgca tggatctttt caagatcaac ggtttttctt tcctttatat
    13501 aatcgatgta gagtgaagtg aggtcgatct tatctgtctt aaggggcagg attgagggaa
    13561 gcacaatgcg ctggtccatc atcttgttgg caatctcctc gatctctgtg gtgatttctg
    13621 accccgcagg acccccgtct gaggggttaa acttgaaccc tgcgtagttt gcagggttgt
    13681 gggaaggggt gaggttaatg gaacacgccg catggagcgt ctcaatggct gcagagaatt
    13741 ccggggtggt ggcttctcct ccgtaccatg tccggattcc ctccttctgg agaatgccta
    13801 tcacctccct ggcaaaatca ggaccgagga atctgttgtc atggcccacg ataatgccgc
    13861 gtctcttaat ctcctcaaaa tccgacaccc ccatggactt catcaccggg gtagcggatt
    13921 ctttgaacat cctcacaatg gccgaagtta ccacgcgcac attgttgaac gtgtagtcag
    13981 ttcctatctc cccccgccag cccgatgtgc cgaacacgat cgttgaggga gaggtattgt
    14041 ccagtgcaaa tttttctata aaagaaagaa gtcccctgtt tctcgaaaca tcagaaagga
    14101 tctccttcca gcaggaagaa gcattctcgt acccctgaac catattcccc tccatccatt
    14161 gcagctcagg aaattatacc agcttccctt tggcagctgc catacctatc ttcccctgat
    14221 gaatattcac caggaggtat agtatcccct tgcactactt aaaaaaaagg aacaatcagg
    14281 tattattaat tataatggaa tgccgcatag tgcggaatac cggataaccc gggaagaaga
    14341 cgtatagcaa atgaaaagac ttgccgtcac aataggagac cctgggggga tagggcctga
    14401 agttgtcctg aaggccctgt catcagtggc agatgaatgt ctgcccatta tcatgggcga
    14461 tctgtccgtg atagaggagg cggttagggc ttttggaatc acccaattca taaagactat
    14521 cagtgatgcc gaggatgcgt cgccctctcc tgaaattctc tggtgcatgg acatgaagtc
    14581 actgaagagt tatgccaggg ggaagcctgc tgctgaaaac gggaaggcct gcgtggatta
    14641 cataaggaag gcggtctggc ttgccatgga gagaaaggtg gacggtattg tcaccgcgcc
    14701 cgtatcaaag gaggcattaa aactcgccgg ctttccatgg cccggccata ccgagatgct
    14761 cgcggagttc accgggacaa aggagtatgc gatgatgctt gtgggaggac ctctcaaggt
    14821 gattctggtg acgatccata ctgcattaaa agatgttccc gctcttgtaa caaaagaaag
    14881 ggtcctgggg actattctcc ttgcaaggaa ggcgtgcgcc atgctcggaa ttaaagagcc
    14941 aaagattgct gtggcgggat tgaatcccca tgcaggagag tcgggggtat tggggaacga
    15001 agaaataaag gagattgtcc ctgccattat gcgggcaaaa gagcaaggca ttgcagttca
    15061 aggcccctat gctcctgatg tcgtctttca taaggcatat aagggggatg tggacatcgt
    15121 ggtctgtatg tatcacgatc aggggttgat ccccctgaag atgatagctt ttgaaaaagg
    15181 ggtgaatgtg accgtgggac tccccatcat ccggacatcc cctgatcacg ggactgcata
    15241 tgacattgca tggaaaggga ttgcggatcc atccagcatg agagaggcca tgaggctggc
    15301 cctgagactc acagtatgat gcggtatgcg ggttgtggga aaagaccggg tatcgtgaga
    15361 gaaggaggga cctatgttgt ttgaaccttt ttcctttagt ggccttaatt ttaagaaccg
    15421 ccttgtccgt tctgccacat ttgaaaaaag agcggatgag gatggttttg tgaccgaatt
    15481 tcttgtggaa ctctacgaag agctcaccat ggggggaatc gggctcatga tcaccgggaa
    15541 cgccctggtc catccctcgg gaagatccgt cccccagatg ctctgcattc atagcgatat
    15601 ctacatccac gagctcagga aacttaccac cgcagttcgt ggattgggcg gtctgattgc
    15661 tctccaactt gtgcacggag gcaggcagag tttccccata ttattgggag gccatgatcc
    15721 catcgccccc tcggcagtat acgacacttc cacaaagatc atgccacggg caatgactga
    15781 cacagagata tgggagatga tcgatgcctt tgctgatgcg gcaagaaggg gaaggattgc
    15841 cggctttgat gcagtgcaga ttcatggtgc ccacggttat cttgtgagcg aatttctttc
    15901 tccccataca aacagacgtg atgactactg gggaggggat gaagagcgga gattccactt
    15961 cctggaagag gtctataagg cgatgagaaa ggaggtgggt gaggattatc ccctaatgat
    16021 aaaaatgaat gcggatgatc ttattgaagg tggattgaag gttgaggaag cggcgagggc
    16081 ggccatgcga ctggaagaac ttggtattga cgccattgag gtgagcggcg ggatgtatga
    16141 atcggatgag aggacggcaa agccggatat tacgagccca gatgacgagg cgtatttcag
    16201 ggatgccggc aaggtattca aggaaaggct gtctgtaccg gtaatccttg taggtggcat
    16261 gaggtcgagg tctgtgatgg aggatgtgat cgagaaggga gaggctgacc tcatctccct
    16321 tgcgaggcct ctgataaggg aacctgacct ccccaataaa ttgaaggaag ggaaggcaaa
    16381 ggctgactgt atctcgtgca atgggtgcat ggaattcgag aggttcgatg tggtgaagtg
    16441 cgtgcagctc cactgattct ggtcacaccg ttccaatttt cagcattatt atggtgtagg
    16501 gaatacttat gactcaacca ttctgaccgt atcaggccgg gtttcagctt tttcctgcaa
    16561 ctcttctgaa tgacgacatg atatgctgat gtgtgtcttt cagaatttta agatggtgca
    16621 agaattatta tcgacaatcg ataatgggga cgtattatgc atccgattaa gcccaaaaac
    16681 tgctcagttt atgaagaatg tgcttctatg cgtttaccgc tatcacaatt aattaatact
    16741 gtaacgagag gcattaaaaa aagttgctcc cataaaaggt gtcttcccag cctaagtctc
    16801 ttcagcctac agtctcttca gcctatggtt gactgaatca ttgtccttcg ctattattat
    16861 gaccaagagg aaaaagacct gacgggaact atcaatgcgc tcattcaagg aaatcaagca
    16921 cgattaccgc ttcacggaag acgatcaaaa gcgcctcgtg gcgcttcagc ctcttatggc
    16981 agagcatacc gaggagatca taagcacgct caacctctgg ttcatgggca ccaaaggcgc
    17041 cttgaccttc ttcacgtccg agacgcttaa aagccatgtc ttcaagaccc agaaggcgtg
    17101 gtttgaggag ctcttttccg ggacttatga aaaccggttt tacgagcaac tcatgaagat
    17161 aggagcggcc catgtgcgcc gaaatgtcga cgtccactac ctgcaacgcg cagccaacat
    17221 catcaagaac acatgcaccg ggattctctc caggggagaa cagccagtgg cggagatcgc
    17281 aagcaatatc atcagtgtcg gaaagatcat cgacatcagc atgaacgtga tcgtgagcgc
    17341 atatatagag gaggagatac gcctctactc ccccgtctat aaagtgaaaa gcgctcttct
    17401 caccttttct gaacgctttt cccaagccac taatatcatg ctggtatttg cgctcatcgg
    17461 tctgactttc ggcgtcggct ggctttttct ccgggacgtg ctcaatatct tctccggtga
    17521 tattgagcat ggcattattt cttcccttgg gtccatgctc ctcctctggc ttatgataga
    17581 actcatgaat accgagatct cgcatctgaa gggtggcaaa ttccatataa gcattttcgt
    17641 cggtgttgcg cttgtgacga tgatccggga gacgatgatc gccaccctcc gccatgaaaa
    17701 accggagtct gtgtattacc taatagctgc tatcctcgtg gtcggcttcg tatactggat
    17761 cgtcgccaag accgaagatc gccacaaatg accatttgac ttcagtaagc cttgcggcca
    17821 acacgacttt cccccatgac tcttgtatcg gctggtgaac gttgcgttac cccacacttc
    17881 ctcaataaat ccgtattagg ttcaattcga acacccgtca ttttgttagt ataaacgcat
    17941 gtcctcgacc cttccggaca ttgtccgtct aatggcaact accccctctc ggtcttacta
    18001 gctcccctta tgcgcgacga tggtcccggc ctcaatcctg ggcgggttcg gaaggactga
    18061 actcagattc tctttcaccc aaactctcgc ttgctcgttt gcatgtatag catccgcaag
    18121 agttttgaaa accgtaatgg atatcatcgc accgtctctc aggtctatcc agtaatattc
    18181 aacgaacccg ggcagcgagg agataaacgg cacgaacttc tcatctacct gttttgcggc
    18241 atttgctgga tcaggaactc cttcatatcg ccgcaccgtc gcatacatct tacgtttcat
    18301 tacgtcctcc ttctgaagct tttttgagct tgagttgtat gatgtcaaaa tcaagcagct
    18361 atttctatac atttcttgct tttttttgtg cattttttgc aggtcttgca ggatatggta
    18421 gagaaaacct caacgagaaa cgtactgaac tactgttctc ttaatgagcg ggggaagggg
    18481 ttctcagcgg ggttttttca gaaatgcggt gagcacgatt gcaattgctg ccaaggctgc
    18541 tgccatggaa aagcttcccg aaaagctccc tgtcctttcc gcaagaattc ctgccattgc
    18601 agggccgatg atctggccga aaccgaagat aaaggtgaca aacccgaatg cctgtcctgc
    18661 cttgtgtgcg cccacataat cgcccactgc cgcagccatg atcgaaggga tgctccacgc
    18721 aacaattcca tacaacccga tggaaaggta aaggaatgca ccggggagtt ttgtcgccac
    18781 aagtatgtaa gcgagcatct ggaggaaaaa gacgatgatc agaccagcct tccgtcccaa
    18841 cttatctgaa agcgtgccga acacggggcc tgagaagaga ctcagaaatc ccacccatga
    18901 ccagaaattg cctgccacgg cctcggaaaa gcccctttcc tgcaccaggg tggtcacgat
    18961 aaaggtggcg taaataacat aggcgaagcc gaagagaaag tagatgatcc ccaaatggta
    19021 aagtacgcct cttttataga tattcatctt cattttgtcg atgatctgtg aataaaaggg
    19081 agcggtgtca tcaatgccga tgggcctgag ccccttctct tcgggagtat tccggatgaa
    19141 aataaggcct ataaaagcga tgaccagtac tattgatcct aacgtgagcc agcttatcct
    19201 ccacccctca agacctgcga gggaattgat gaaggggatg agctttcccg aaataataat
    19261 ggcaaagccg cttccgatga cgatgaatcc cgctgccttt ccccttatgg tactcgcgaa
    19321 ccagtgggag accaggctca tcacagggac attggcagct ccgctcccga taccagtcag
    19381 gaagtagagc acgagcacat acctgaaact ccttgcctgg ctcaccagta tcatggagat
    19441 tccgacaagg agcagagcaa tgaagatgag ctttcttgag cggatgcgta ttgcccaaaa
    19501 gccgcttatc agcacagagg taagatagcc gaaaaaattt gcggtgctga tgaaccccat
    19561 ctgtgaatag gtgaggttca gggtcgaggt catggagggt aggagcatac ccagggcaaa
    19621 cctgccaaag ccgagacatg caaagatgca gagtgtgccg gtaaagacaa tgacccaccc
    19681 gtaatggaaa gggatctttg aggtcgaggt ttccacatag gaatatacca ttgtttttga
    19741 aaaatgcgaa agagaagggc gaaatataaa aaaagcttga caaagaacta ttcgccctgt
    19801 tattgttgat attagtaatc aatattaaaa gagcaattga tgattattga tgaggcaaga
    19861 gatacctttc gggactatgt aaggaagaag ggattgcgca atacccggca gagggatgag
    19921 atcttgaggg cattcctttc tgagggcaag catattacca ttgatgatct ttatgcaatt
    19981 gttaagaagg agaatcccga gatcgggtac gctacggtac atcggaacct gaaattgatg
    20041 tgcgagtgcg gtctggccga cgagataaag gtcggcaaca aaaagacacg atatgagcaa
    20101 aaacttggac atgcgcatca tgaccatctc atctgcctga aatgcgggaa atttatcgag
    20161 gtcaaagatg aaaagatcga acggcttcag gataaacttg ccgaagggaa agggtttatt
    20221 cccatgaggc ataagttaga gatatacggt gtatgcaaaa agtgcaaata acattttttt
    20281 ggcctattaa tgataatgat tatcattatc attaactcat agcaacagag gacatgcaga
    20341 gatagattct agacagtaca gaacgtagga aactaagggt gtttaaaagg ttatttttgt
    20401 cagcagtaat agtgctaggt acggcagggg cagcctttgc tgccattccc cttataaccg
    20461 atgatacggg cactcagggg agagggaatt ttcagcttga gctctttggg gaatacggac
    20521 atgatcgcga aggaataatc acgacaaaga cgagcgacgt ctctgcaaca ctgacctatg
    20581 gactcattga gaccgttgac attgttttag gtatccccta ccaggcgtgg agttccgata
    20641 attctgagtc tttggtaaaa gggaacggca tcgctgatct gtcgctcgag gccaagtgga
    20701 ggttttacga aaaagagggg ctaagccttg cactcaaacc cggcttcaca atccctacag
    20761 gggataccga aaaggggctc ggtgcaggga ggcctaccta tcacctcctt ttcattgcca
    20821 caaagcaaat gaaaccatgg gaatttgata tgaacgttgg ctacctctat aacgaaaaca
    20881 cggccagcga gcgaaagaac atatggtctg cctctctgga tgcacaggtt gaggttgtca
    20941 aggacctcaa gcttgcattg gatgtcggcc tcgcgacaaa ccctgacagt tcatcagaca
    21001 cgcctccggc atatgtgctc ggcgggctga tctggtcgct cagagagaac cttgacattg
    21061 gtctgggtgt gaaggtcggt ttaaccaaac ctgaaacgga tatctctgtc cgtggcggca
    21121 taacatggag atttta
//
LOCUS       DATKOU010000253         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1904838, whole genome shotgun sequence.
ACCESSION   DATKOU010000253 DATKOU010000000
VERSION     DATKOU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904838"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VMU21_12110"
     CDS             complement(<1..553)
                     /locus_tag="VMU21_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO78308.1"
                     /translation="MALLVLLVTYLAVDRAYLGFLPDPFKAFRISSRIRELKQVVSIN
                     PYDGRSLKELGMCLVEKGDYQNAMRFFAKAEEKMSDDAEFNYYYGISTARSGDVAAGR
                     QIFEEAVKASPTLKYGEPYLMMAEAYMNNADYEGALSLLERFENIHSSSSRGLYRMGL
                     VKLKLGCRNEGVEYLKKSVQAFKA"
     gene            complement(632..754)
                     /locus_tag="VMU21_12115"
     CDS             complement(632..754)
                     /locus_tag="VMU21_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78309.1"
                     /translation="MKFKERLAYAYDDALIAHIPKNLHEALHTYTRGDEGNGPN"
     gene            1076..1273
                     /locus_tag="VMU21_12120"
     CDS             1076..1273
                     /locus_tag="VMU21_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78310.1"
                     /translation="MKHKHDFESIYHPNPSSEVKDHGILAVEIRKCKGCQKEIPFVLI
                     KNKWLPLFEDLGSDDQDILLA"
     gene            1647..2537
                     /gene="prpB"
                     /locus_tag="VMU21_12125"
     CDS             1647..2537
                     /gene="prpB"
                     /locus_tag="VMU21_12125"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:NF008455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HUO78311.1"
                     /translation="MTRPLSPGARFRAAVREEKPLQVVGAVNAYAARLAEHAGFRALY
                     ISGGGVAASSLGVPDLGISTMDDVLTDVRRITDITELPVLVDIDTGWGGAFNIGRTIR
                     SMIKFGAAAVHIEDQVQAKRCGHRPGKAIVSKGEMVDRIKAAVDAKTDPDFVIMARTD
                     ALAVEGLHSAIDRASACVEAGADMIFPEAITELNMYREFARAVKVPILANITEFGSTP
                     LYTVEELRQADVSLILYPLSAFRAMSKAALSVYDAIRKEGTQKNVMGLMQTRTELYEH
                     LDYHAFEQKLDNIFSKEEQE"
     gene            2534..>2792
                     /locus_tag="VMU21_12130"
     CDS             2534..>2792
                     /locus_tag="VMU21_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HUO78312.1"
                     /translation="MSTGTITEQTAMKVKKSVALSGVVAGNTAVCTVGRTGNDLHYRG
                     YDIVELAKGATFEEVSYLLIHGDLPSQSELKGYMNKLKALRG"
BASE COUNT          737 a          670 c          672 g          713 t
ORIGIN      
        1 aagctttgaa ggcttgtact gatttcttca gatattctac gccttcattc ctgcaaccga
       61 gctttagttt cacaagtccc atcctgtaca accctcttga actggaagaa tggatattct
      121 caaacctctc aagcagggac agagcgccct catagtctgc gttattcata taagcctcag
      181 ccatcataag atagggttct ccatatttca aagtgggtga ggcctttacc gcttcttcga
      241 agatttgcct tcccgcagca acgtcgccgc ttcgagcagt agatataccg taataatagt
      301 taaactcagc atcatccgac atcttttctt ctgcctttgc aaaaaatctc atggcatttt
      361 gataatcccc tttttcaacc aagcacatgc cgagttcttt taaagaccgc ccgtcatagg
      421 gattgatcga gacaacttgt ttgagttctc taatccttga agagattctg aaggccttga
      481 aaggatcagg caaaaagccc agatatgctc tatccactgc aagataagtt acaagaagaa
      541 cgagaagcgc cacaatcgga ctcccggtca ccaagtacaa gatataaaaa ataaataatt
      601 ttcccatttt cccttttctt gcttcaggcc tttagtttgg cccgtttccc tcgtcccccc
      661 tcgtataggt gtgaagcgct tcgtggaggt tctttggtat gtgggcaatg agggcatcat
      721 catatgcata ggctaacctc tctttgaact tcatgccacc tgttttttgg acgtgagaaa
      781 ggagggcctg ttggtcagga caatggacta aaacgctttc cgcaatggcg cggatggtgc
      841 tgaagtattg atcagttacc ttgactctga atagagcgta agtcttgcct gaaactgact
      901 tgtatggtct atccactttt ctattataac ccgcagaaag gacccaacgg aaggaggcct
      961 ctggagcgac caaaggatcc ttccgcaaat cgtaccgcac agttcagtat tctgtgcacg
     1021 catagctcga caagatatgt gaattagagt atgcttgaca aaggaggtca gggcaatgaa
     1081 acataaacac gattttgagt ccatttatca tcccaaccct tcatctgaag tgaaagacca
     1141 tggcatcctt gcggtcgaga taagaaaatg caaagggtgc cagaaggaaa taccatttgt
     1201 gttaattaaa aacaaatggc tccctctctt tgaggatctg ggctctgatg atcaggacat
     1261 acttctggcc tgagtgcggg atttagagat ataatattgc gtgcaataat ttgcatttaa
     1321 cgcaagttat tgcgtataaa tgagcataag aattaaattt tccattttcc cgattttcct
     1381 tcttacggcg cttatttatt attgcttaca aagtgttaca gcctattctt cttatttttt
     1441 tctgcaatac tgactttggt ataacatctg gcacgagacg tgatagaggg gtttgctgtt
     1501 tcgggaaaaa cctggtgaaa tcccaaagca ccaggaagca cgattaaaaa aacaagaatg
     1561 cactgcgggg gaggtagttt cttgaaaaag atcattgccg catatccttg ccagaaaatt
     1621 catttgagca tacggaggat ccatgtatga ctagaccatt atcaccaggg gcccgtttcc
     1681 gggctgcggt gcgcgaagaa aaacctctgc aagtggtggg tgccgtcaac gcttatgcag
     1741 cccgtctcgc cgaacatgcg ggcttcaggg cgctctatat ctcgggaggc ggggtggctg
     1801 cgagttctct gggtgttccg gatcttggca tatccaccat ggacgacgtc cttactgatg
     1861 tcagacgtat caccgatatc accgaactcc ctgtgctggt ggacatcgat accggctggg
     1921 gcggcgcctt taacatcggc cgcaccataa ggtccatgat aaagttcggc gccgcggcag
     1981 tgcatatcga ggaccaagtc caggcaaaac ggtgcggtca tcgcccgggg aaggccatag
     2041 tgagcaaggg agagatggtg gaccgtatca aggcagcggt ggacgcaaag accgatcctg
     2101 actttgtgat catggcaagg accgacgccc ttgcggtgga aggccttcat tctgccattg
     2161 accgggcctc tgcctgtgtt gaggcagggg ctgacatgat tttccctgag gccataacag
     2221 aactgaacat gtatagggaa ttcgcccggg cggtgaaggt gcctatactt gccaatatca
     2281 ccgaattcgg gtccacccca ctctacacag tggaggaact gcgccaggcg gatgtctcac
     2341 ttatcctcta tcccctttcg gccttcaggg ctatgtccaa ggctgccctt tctgtatatg
     2401 acgcgatccg caaagagggg acccagaaga atgtgatggg tcttatgcag acccgcactg
     2461 agctatatga acatcttgac tatcacgcgt ttgaacaaaa acttgacaac atcttctcaa
     2521 aggaggaaca ggaatgagca caggaacaat cacggaacag actgcaatga aagtaaaaaa
     2581 atctgttgcc ctctcgggtg tggtggcagg caataccgct gtctgcaccg tgggacgcac
     2641 aggcaatgac ctccattacc gcgggtacga catcgttgag ctggcaaagg gcgccacctt
     2701 tgaagaggta tcctatctgc tcatccatgg agatctgccc agccaatcgg aattgaaagg
     2761 ctatatgaat aaattaaagg cccttcgcgg ac
//
LOCUS       DATKOU010000254        12964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1528510, whole genome shotgun sequence.
ACCESSION   DATKOU010000254 DATKOU010000000
VERSION     DATKOU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528510"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VMU21_12135"
     CDS             <1..577
                     /locus_tag="VMU21_12135"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO78313.1"
                     /translation="HASIELVETDVAFGPRTAVICDAHSLPFENASVDGVVAQAVLEH
                     VADPYKCVEEIYRVLKKKGLVYAETPFMIPGHFEQYDFTSFTHLGHRRLLRRFDEIDS
                     GAVCGPGMALAWSYRSFLTSFTTSKIVRKFIYTFAAFTSFFFKYFYSFLIDKPGTIDA
                     AAVTYFLGQKSDAVLSDRKLLKQYRGLARHG"
     gene            complement(1228..2247)
                     /locus_tag="VMU21_12140"
     CDS             complement(1228..2247)
                     /locus_tag="VMU21_12140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO78314.1"
                     /translation="MNFRKMVSFIYDSLFRREQLHFWRNYSRAVEGFYKIHFLHAAKS
                     SGLLSALESPTDLAGLATKLKTERIDLLEVLCELGVKCGYMYRADDRFALSCDLMKLL
                     KANGLDAILAYTEEISTLQGTALNDLPSNLHSFSPVTYLEGKGILIARSSILFEHDLK
                     KLIRAAMPQTGAVRILEIGCGSGIYLFHASEINSEVSGIAIDKEDDAIKLASQNLSRW
                     KLSGKFQLIKQDILAFESEGKTFDVITLYNNIYYFKEEEQIKLFNKLRGLLIPGGKLV
                     IAAAMQGGHIRSVVLDLLVRSSIPRGSLPTKDNLIKQLSSCGFNKINAMSLNPSGSYI
                     GIIAQ"
     gene            complement(2275..2910)
                     /locus_tag="VMU21_12145"
     CDS             complement(2275..2910)
                     /locus_tag="VMU21_12145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUO78315.1"
                     /translation="MDSVIIRNASKKDLEGIMDVELDWPEEQRAGSDKFVARLDKCPQ
                     GFFVAEVDGKIVGTSTSCVIRYDPFHPEVHKSWDIVTNDGYIHRYEEIENPNALYIVS
                     IGIKRNYRAKGIFQGFAKAQVDLAIKVGLPYVLAGAIMPGYDSYCKKYGDITAEEYGF
                     TRIGHRLADPFLEVWRGLDFHVPDNRHIMTDYYSDTRSRNYSALVVYEIKK"
     gene            complement(2903..4357)
                     /locus_tag="VMU21_12150"
     CDS             complement(2903..4357)
                     /locus_tag="VMU21_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78316.1"
                     /translation="MGTLEIIKKFIESNWIIERGAREVLERLPVRLRYGISYGPTFRS
                     WLGFLKESEQWNEDRLEAYQVEQLRDLLMHAGGNVPYYRKLFGEYGFKPERTQSLDDL
                     KVLPYTDKEIVRDNIEGFLAENVPRKSLFRKATSGSTGIPLALYSDKAAEEKHWATVV
                     HAWSKVGYTPGSRILTIWGNITRGGKDNLPFKRYANQLIISHHYFDSERWIKQYLLMI
                     RQFRPEIIFSLPSTLSSFSNYINEGLIPPLDGIKACIVVNEALYSWQRKLIEENLNTR
                     VFSTYGMVEKNVYASGCVSSSRYHVYPQYGVPEVFQVGDGIFEIVGTGLINYAQPLVR
                     YRTFDRGVLNATKCPACNSLYKSLNLIEGRLGEVLIDKNGKIHTPIDVGVDSKAFEDI
                     KLFQFYQDTPGKILLRLVKKRACSDSIAIRINEELMTVLGLWGKENKMEIEIVFVDEV
                     NRSPSGKFTIVEQKLDVRRFLNTKEVTASCASHG"
     gene            5042..6325
                     /locus_tag="VMU21_12155"
     CDS             5042..6325
                     /locus_tag="VMU21_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HUO78317.1"
                     /translation="MNLFKNVIASPPPSVVWTNTRSGRRSKDPNQLSFDDFLPSIAQS
                     VSPAKVSPQSAMETAFSRNYRIKETDLIGEGSLKQKYRQNVEAIRLLKLIESEGREAS
                     SEEKSILVRYSGWGGIPQVFDRYNDDWEKEYEELEGLLTEDEYDSAKASTPNAHYTPP
                     LIIKFIYDVLSRMGFRGGRILEPAMGVGHFFGMLPLGIAFESRLCGIEIDTVSGRISK
                     LLYPDSDIRITGFEDAKLPDNFFDLALSNVPFGNYRVHDPRYDRYRFSIHDYFFAKAI
                     DVVRPGGMIAFITSMFTLDKKESRLRKYLFERADMVGAVRLPNAAFKGIANTSVTSDI
                     ILLQKRQEGTIPASCSWIETKPYLIKKGMEEEINEYFIDNPHMMLGKLAVGKQMYGRN
                     EVTLNPDGKPLQVALKEAISHFPEGLLNCFTGSTR"
     gene            6453..7247
                     /locus_tag="VMU21_12160"
     CDS             6453..7247
                     /locus_tag="VMU21_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUO78318.1"
                     /translation="MEDKDEQLSIDVPEVFRTEDIDLDKIITNGDMKESVCANILKSL
                     SSMKRVIQPVLVIEEGETYRLVFGQRRVLAARKAGHSKILATIFPAGTPEEVLSMCVL
                     VENMNRSPNPASEAEALSKVMTAYKWSKADVRRNLGIPARHVSSRLKLLDRLIPEFFQ
                     RLKEGSISFSLAKNLCNLSEEKQRDLLRKERITLEEAEDAVREASVGNLIPDELFRMP
                     KGAEEEDISAEDHLREAITCLERAIATTGNGKKEKMEEALKLLKEV"
     gene            7251..7919
                     /locus_tag="VMU21_12165"
     CDS             7251..7919
                     /locus_tag="VMU21_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78319.1"
                     /translation="MKEPRSITGITRGCGLRKPGGLYVCSGLSPYGSPLENFIVDPPL
                     PYNSGPFRAPIVFEKEGRKHIMMWVGAEYYPYCSDFIEEVRKYGASKRVPNNFPIEEL
                     ERGSMLFLVHPKAMVADFEYLPMVEYCPKADPSHWAANEEYCLGHSYQIAEPNVGINL
                     RKLGDTEYTVYPHTMSAAEYEFSPGIFLRLPITHFDHIVHKGKTNPKITSKRAKLPIN
                     LEKE"
     gene            7970..8383
                     /locus_tag="VMU21_12170"
     CDS             7970..8383
                     /locus_tag="VMU21_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78320.1"
                     /translation="MNPFSHEHGMKESIETFFRALYGLAELQSTRKNSATSDPKECSA
                     FISLMTELGSLVKDWDTVAEDLLERNDYTVQLWTDKKSRAKVLVCLQEICPGRYLPAF
                     FVPVPAGEDITEILDDVARRISAGAMHEEQVSAAP"
     gene            8521..8901
                     /locus_tag="VMU21_12175"
     CDS             8521..8901
                     /locus_tag="VMU21_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78321.1"
                     /translation="MSQGQQQIEGHSAKTGERGMKHTRKRRANDDTPLIDHIIAAMKE
                     YKAITIKIAEELGFEHRGEPSSDEVKRYLLDIPMDPDDYALLHQIGGKPTLCHAILEA
                     VGAADHEIEEASGLAAFAYDAVGV"
     gene            8939..9163
                     /locus_tag="VMU21_12180"
     CDS             8939..9163
                     /locus_tag="VMU21_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78322.1"
                     /translation="MTLSKILPREIRIALYGVVLDTDTKKLKERVREMRQMRQRRKAV
                     LSLCSQCWFKDYCLIRNGLDEKRTAIEKAA"
     gene            9199..9483
                     /locus_tag="VMU21_12185"
     CDS             9199..9483
                     /locus_tag="VMU21_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78323.1"
                     /translation="MPGSTALRQKERRKVMRKELREIEKMLHPCPVFDGIEVLHEHRG
                     RRIVFTAWREDTQIRGKEVAFPRKSVLYGPADFYEANKRDFQWLVAGSSG"
     gene            9557..9973
                     /locus_tag="VMU21_12190"
     CDS             9557..9973
                     /locus_tag="VMU21_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HUO78324.1"
                     /translation="MIISEHKGEKIDGPGKVADIIRALLAAEHETDREREHFWAIGLN
                     TKNRIKYIELVSLGTLSNSLIHPRETYRLAVMNAAAQLIIAHNHPSGDVAPSRDDIAI
                     TERLKAAGEIMGITLLDHVILGSDGTFTSMKEAGHL"
     gene            10044..10394
                     /locus_tag="VMU21_12195"
     CDS             10044..10394
                     /locus_tag="VMU21_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78325.1"
                     /translation="MEDWRHFLLYAKNYYLKTETISDLLKILSHETGLDPRSVKLWDV
                     YEVLAGRFVKYTRGDQKKDFFMRMFSEGDSVKIGDVVLQLLAALSVQKVRDGETVLID
                     LGEPDPSVLPLNKY"
     gene            10411..10806
                     /locus_tag="VMU21_12200"
     CDS             10411..10806
                     /locus_tag="VMU21_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78326.1"
                     /translation="MSSANAMKDLTARVEEGIKVTKAAIARGDALRVGDCLRHFAALN
                     APIDKIYEAIEECPVSLIEEAGYCLIVKSALLGREIILGKDIPWQEVISLLQKGLTGK
                     DLMTVLETRELFEGKVVLTDSPSLFDTPA"
     gene            10952..11488
                     /locus_tag="VMU21_12205"
     CDS             10952..11488
                     /locus_tag="VMU21_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78327.1"
                     /translation="MNKTMNASAETGQFIVLDLFNPDIVNVECNEKGETLYFDTEEKA
                     MEHAEDVQDPLVIELSLGGANVVRSTHVLLNVVLFPENEQSDDIPSGKENHIYLNGKY
                     ISSRCNDEYDDYTVTWEGYCGILNGLKMAIPGLTCKDILIDDVRLTEILGENWREYYE
                     EGSLWARVERQLGVQVIE"
     gene            11621..11836
                     /locus_tag="VMU21_12210"
     CDS             11621..11836
                     /locus_tag="VMU21_12210"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HUO78328.1"
                     /translation="MNPRIISNPNICGGEPCIKGTRIPVHVILSHLAAGEDYETILKH
                     FPRINKEDILACLEYASFLATEKTVAA"
     gene            11833..12183
                     /locus_tag="VMU21_12215"
     CDS             11833..12183
                     /locus_tag="VMU21_12215"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HUO78329.1"
                     /translation="MKIIVDESVSYGLVAVLRTTGHKVIAIAESATSGTVDEEIYNLV
                     IQEGAILISRDFHFTNALRFPPERTRGIIYIRHGNLMALEEIEIVQRFLSAHRHDEYN
                     GRLATLYKDSVKIR"
     gene            12346..12657
                     /locus_tag="VMU21_12220"
     CDS             12346..12657
                     /locus_tag="VMU21_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78330.1"
                     /translation="MSYSIDSLSGESLSVGCYGEVYYSEVDAQNTCGLEFCCIGTLHT
                     QTEIEGALFVNEVRLSFNNRKIEDDAGAIHKVDRHSSVRRQYADGVCSLKGKDTFVID
                     D"
     gene            12661..12792
                     /locus_tag="VMU21_12225"
     CDS             12661..12792
                     /locus_tag="VMU21_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78331.1"
                     /translation="MFSKNMQPFTFSPVGFDYLADCSNGQLRGKAIFNAYNPVAKAM"
BASE COUNT         3564 a         3051 c         3116 g         3233 t
ORIGIN      
        1 tcatgcatcg attgagctcg tcgaaaccga tgtcgccttc ggccccagga cagctgtaat
       61 ctgtgatgct cacagcctcc catttgagaa cgcttcagtt gatggggtcg ttgctcaggc
      121 cgtgctcgag cacgttgccg atccatataa atgtgtcgaa gagatatacc gcgtgctcaa
      181 aaaaaagggc cttgtgtatg cagaaacacc ttttatgatc ccgggacatt ttgaacagta
      241 cgactttacc agctttactc acctcggtca ccgccgcctg ttgcgccggt tcgatgagat
      301 agacagcggc gcggtctgcg ggccggggat ggcgcttgca tggtcctata gaagtttcct
      361 cacgagcttc accacatcga agatagtgcg gaagttcatt tatacattcg cggccttcac
      421 ttctttcttc ttcaagtact tttactcttt tcttatagat aaacccggca cgattgacgc
      481 cgcagcagtc acttatttcc tcgggcaaaa gagcgatgcc gttctctctg accggaaact
      541 tctgaagcaa taccgggggc tcgcaagaca tggataaatg acttgtcgac ctcattccaa
      601 cctgatgctc aacctcctcc acgtcgcggc tgttcataaa cggtacgttt ggaagcgttt
      661 agtaggagtg gcactcggca cgctgttcca ttcacggtaa tgttactttc caagagaatc
      721 aaacatcatt gacaatttgc gtcacgtgtg agatacagga agcagccgaa aaattaattt
      781 ggcgctctta taagatcttt ttggagcaag gtacgggata aacactcgtt tacgaaaaaa
      841 ggatgtctat cgcaaccttc cttcctgtat tcgaacccgt gcaatccctt tcagaggtgc
      901 agtgttaagc gggaataatt ttttaatcaa aactaatgcg atgagggctt cgtgtagtaa
      961 cccgcagcct ataagcgctt agcaaagcgg agaaatcttc cagaaaactc atcaggcgga
     1021 gacattctgc tcatgctggg caatcaatct ctgcacaaga tagagcttcc ataaaaaatg
     1081 agggacgtta cctcgaagcc aatactgcaa cagatgatga ttccacaagc gtcttaaggt
     1141 catagttcaa gataatggat attcggtaaa gccttcttcg ggaatgttct gcctacttca
     1201 ccttctcctt gagttctttc atcgctctta ttgagcaatg atcccaatat aactgccgct
     1261 aggattcaag ctcattgcgt tgatcttatt gaatccacag gaggacaact gtttgattag
     1321 gttatctttt gtcggaaggg aacctcttgg gatagatgat ctcacgagca gatcaagcac
     1381 aactgacctg atgtgtccac cctgcatcgc cgctgcgatt acaagctttc ctccaggtat
     1441 caggagaccc cggagcttat tgaatagttt tatttgttct tcctccttaa agtaatagat
     1501 attattatag agtgtaatga catcaaatgt tttcccttca ctctcgaatg ccaatatatc
     1561 ttgcttgatg agctgaaact tgcccgacaa tttccatctg ctaaggtttt gtgaagcgag
     1621 ttttatagcg tcatcttctt tgtcaatagc tatgccgctg acttcggaat ttatttctga
     1681 agcatgaaag agatagattc ccgaaccaca acctatctca agtattctta ccgctcctgt
     1741 ttgaggcata gcagccctaa tgagcttttt caagtcatgt tcgaaaagta tagaagagcg
     1801 tgcaatcaaa ataccttttc cctcgagata tgttacgggg gaaaaactgt gaagattaga
     1861 aggcagatca tttaatgcag taccctggag cgtgcttatc tcctctgtat atgcaagaat
     1921 agcgtcaagg ccattagcct tgagcaactt cattaggtca caacttaatg caaatctatc
     1981 atccgcccta tacatatatc cacatttaac acctaattcg cataatacct ctaaaagatc
     2041 aatccgttca gttttaagtt ttgttgccag accagcaaga tctgtcgggc tctcgagtgc
     2101 gctgagcagg ccagaagatt tagctgcatg aagaaaatga atcttataga acccctcaac
     2161 ggctcttgag tagtttctcc agaaatgcaa ctgctccctt ctgaaaagag agtcataaat
     2221 gaaagaaacc atttttctaa aattcatatc tgcattccag gaactgctaa gtcgtcattt
     2281 tttgatttca tagaccacta aggcagaata attcctgctc ctagtatcac tataataatc
     2341 ggtcattata tgcctattgt ccggcacatg aaagtcaagc cctctccata cctcaagaaa
     2401 cggatcagca agtctatgcc caattctagt aaagccatac tcctcagccg tgatatcccc
     2461 gtatttctta caataggaat cgtatccggg cataattgcg cccgctaaga catagggcaa
     2521 acctacttta atcgctaaat ccacctgtgc tttggcaaag ccctggaaaa ttcctttagc
     2581 gcgataattt ctttttattc ctatcgaaac gatgtaaagg gcattaggat tttcaatctc
     2641 ttcataacga tggatatatc catcgtttgt tacaatgtcc catgatttat ggacttcggg
     2701 atggaatggg tcgtatctga ttacacaact cgtagatgtc cccacaattt taccgtccac
     2761 ctctgccaca aaaaaacctt gaggacattt atcaagtctt gcgacgaact tgtcactccc
     2821 tgccctctgt tcctccggcc agtccagttc cacatccatg atcccctcaa ggtctttctt
     2881 tgaagcattc cttataatca cactatccat gcgaagcaca cgacgcagta acctcttttg
     2941 tgttcagaaa ccttcgcaca tctaatttct gctcaacaat agtaaattta ccagagggcg
     3001 atctgttcac ctcatccacg aaaacgatct ctatctccat cttattttcc tttccccaga
     3061 ggccgagtac tgtcatcaat tcttcgttta ttcttatggc aatagaatcg ctacatgccc
     3121 gcttctttac aagcctcagt aaaattttcc ctggcgtatc ctggtaaaat tgaaacagtt
     3181 taatatcctc aaatgctttt gaatcaaccc ccacatctat tggagtgtgt attttgccgt
     3241 tcttatctat aagtacttca cccagtctcc cctcaatcag gttaagagac ttatagaggg
     3301 aattacatgc cggacatttt gtggcattta agactcctct atcaaaagtt ctataccgca
     3361 ctaaaggttg cgcataattg ataagccccg ttccgacaat ttcaaagatg ccgtctccga
     3421 cctgaaaaac ttctggcact ccgtactggg ggtaaacatg atatctcgac gaactcacgc
     3481 atccggatgc atagacattc ttttcgacca ttccatatgt tgaaaagacc ctggtattca
     3541 ggttttcttc aatgagtttc ctctgccatg aatagagggc ctcgttcact acgatacaag
     3601 ctttaatgcc gtcaaggggt ggtatcagtc cctcatttat ataatttgag aaggaagata
     3661 aggttgaagg aaggctaaat ataatctcag gtctaaactg ccttatcata agaagatatt
     3721 gcttaatcca tctttcacta tcgaaataat ggtgggagat gatcaattga tttgcatatc
     3781 ttttaaatgg aagattatcc tttccgcccc ttgtaatgtt tccccaaatg gtcaatatcc
     3841 ttgaaccggg agtatatccc accttgctcc atgcgtggac aaccgtcgcc cagtgttttt
     3901 cttcagccgc cttgtctgag tacagagcaa ggggtattcc ggtagatccg ctcgtagcct
     3961 ttctgaataa tgattttctg ggaacatttt ctgcaagaaa tccctcgatg ttgtccctca
     4021 cgatctcttt gtccgtataa gggagcacct tcaagtcatc caggctctgg gttctttccg
     4081 gcttaaatcc gtattctccg aaaagcttcc tatagtatgg cacattcccc cccgcatgca
     4141 tcagtagatc cctcaactgc tccacttgat atgcctcaag cctgtcctca ttccattgtt
     4201 cggattcctt caaaaatccg agccaagatc tgaatgtcgg tccataggaa atgccgtacc
     4261 taagcctcac cggaagcctc tcaaggactt ctctcgctcc cctctcgata atccagttag
     4321 attcaatgaa tttcttaatg atttctaatg tacccatctt ctttgctttg tctgcgttat
     4381 tttgttatat gaaaggtatc atatttagga tagtttaaat gcacttcttc gaatattata
     4441 gctcaacaga tgggggagcg tcgcctttcg taaaaacatg ggcgaatcct gccccaaggg
     4501 ctatcagcaa gaaatgtgta ctacaacatg aacctttcaa tctcattttt acgggggaga
     4561 tagatggctt ctatcggaaa gtatcactgt gtcaaagcag caccagtctc aacttgtcgg
     4621 tgttagtgat acagaacaat ttgagagaga agtatttctt tccgaaggaa acaagcgggc
     4681 tcgactttgt cgttaatatc ctccagatca agtattagca actacattcc aagtttgtcc
     4741 ccctcaagtc ttacggctgt ttttaaagag tagaatcaat gcccgttcaa taatccgtat
     4801 cagaggatac tcgctcagcc ttgaatatct attcactaag agctgagatc cggtcattca
     4861 gaagctccgc tcatttatcc tatcaaggtc aggttttttc aaataaggcg ctgtcatagt
     4921 agccgcgtcg taaataagat ggcgccttat ttattaacaa gttcatcaca ttaagctgcc
     4981 accgtgtcct tttcattaaa tttcagcatt aagaaacctt ccatcaataa aaggaggatc
     5041 attgaatctt ttcaagaatg ttattgcctc acccccgcct tctgtcgtat ggacaaatac
     5101 acgcagcgga aggcggagta aagacccaaa ccagctatcc tttgacgatt tcctcccttc
     5161 cattgctcag tccgtttcgc cggcaaaggt atcgcctcag tctgccatgg aaacagcttt
     5221 cagccgcaac tacagaatta aggagaccga ccttatcggg gaaggctcgt tgaaacagaa
     5281 gtacaggcag aacgtcgagg cgatccgtct cctcaaattg atcgagagcg agggacgcga
     5341 ggcgagttca gaggagaagt caatattggt caggtattcg ggctggggag gaatccccca
     5401 ggtcttcgac cgctacaacg acgattggga gaaggaatac gaggaacttg agggtctcct
     5461 tacagaggac gagtacgatt cggcgaaggc gagcacgccg aacgcccact acactccacc
     5521 tctcatcatc aagttcatct atgacgtgct gtcgagaatg ggcttcaggg gaggaaggat
     5581 tctcgagcct gccatgggcg taggacactt ctttggaatg ttgcccctgg gcatcgcttt
     5641 cgagagcagg ctctgcggta ttgagatcga cactgtgtcg ggaaggatat cgaaacttct
     5701 ttaccccgac tccgatataa gaatcacggg ttttgaagat gcaaagcttc ctgacaattt
     5761 cttcgacctc gccttatcga atgtcccctt cgggaattac agggttcatg acccacgata
     5821 cgacagatac aggttcagca tacacgatta cttcttcgcg aaggctatag atgttgtcag
     5881 acccgggggc atgatcgcat tcatcacgag catgttcaca cttgataaaa aagaaagccg
     5941 cctccggaag tatctcttcg agcgggcgga tatggtcggc gctgtccgcc ttccgaacgc
     6001 cgcgtttaag ggcattgcca acacctctgt tacgagcgac atcatcctgc tccagaaaag
     6061 acaggaaggg acgatccctg cttcctgctc ctggatcgag accaagcctt accttatcaa
     6121 gaagggaatg gaagaagaga taaacgagta ttttatcgac aatccccata tgatgctcgg
     6181 caaactcgct gtcggcaagc agatgtacgg gagaaacgag gttaccctga atcccgatgg
     6241 gaaaccgctc caggttgcgc tgaaggaggc catcagtcat tttccagagg gcctgctcaa
     6301 ctgcttcaca ggcagtacga gataggaaag cagcttgacc tcgacaagag agagtcaacc
     6361 ggaggactca gcgatgaaga agttatggag gcggcgtaag ggcaggggac ggcctgccct
     6421 tttccttgcc tcaatcagta aggaggaagt gaatggaaga taaagacgaa cagttgtcca
     6481 tcgatgtgcc ggaagtcttc aggacggaag atatcgacct ggacaaaata attacgaatg
     6541 gagacatgaa agaatccgtt tgcgcgaaca tcctcaaatc cctgtcgtcg atgaagaggg
     6601 tcattcagcc cgtactcgtc atcgaggagg gagaaactta caggctcgtt ttcggacagc
     6661 ggcgggtgct ggcggcgaga aaggcgggtc acagcaagat actcgcaacg atctttcctg
     6721 caggaacgcc cgaagaggtt ctttccatgt gcgtccttgt ggagaacatg aatcgttctc
     6781 cgaaccccgc atcagaggcc gaggcccttt caaaggtgat gacggcatac aagtggagca
     6841 aagcggacgt cagaagaaat ctcggcatac cggccaggca cgtatcatcg aggctgaagc
     6901 tcctcgacag gttgataccc gaattcttcc agcgactaaa ggaaggaagc atatcctttt
     6961 ctcttgcgaa gaatctctgc aatctgtccg aagaaaagca gagggacctt ctcaggaagg
     7021 agaggataac cctcgaagag gcagaggacg cggtaaggga agcgagtgtc ggtaacctta
     7081 tacctgacga gctattcaga atgccgaaag gcgctgagga agaagacatc agcgctgagg
     7141 accatctgag ggaagcgatc acctgtctcg aaagggcgat cgccaccaca gggaacggga
     7201 aaaaagaaaa aatggaagag gctctgaagc ttctcaagga ggtttgaaaa atgaaggaac
     7261 cgagaagcat aacgggcatc acccggggtt gcggactgag aaagcccgga ggtctttacg
     7321 tatgcagcgg cctcagtcct tacggctctc ccctcgagaa cttcatcgta gaccctcctc
     7381 ttccatataa ttccgggccg ttcagggccc ccatcgtctt tgagaaagag ggcaggaaac
     7441 acatcatgat gtgggtggga gcagagtact atccctattg ctcggacttt atcgaagagg
     7501 tgagaaaata cggtgcctca aagagggtcc ctaacaattt ccccatcgag gagcttgaaa
     7561 ggggatcgat gctttttctg gtgcatccaa aagcaatggt cgcagacttc gagtatcttc
     7621 ccatggtcga gtattgtcca aaagcagatc cttcgcactg ggccgcgaac gaggaatatt
     7681 gcctcggtca ttcctaccag atagcggaac ccaacgtggg aatcaattta aggaagctgg
     7741 gagacaccga gtacacggtt tatccccaca ccatgtcggc cgcagaatac gaattctcac
     7801 cggggatatt cctgaggctt ccaatcacgc acttcgatca catcgtgcac aagggcaaaa
     7861 ctaacccgaa gataacgtca aagagggcga aactgccgat caatctggag aaggaatagt
     7921 ctgtgcggag gttccggcaa ccagataaga gcagaaaggg aggaaaccaa tgaatccatt
     7981 ttctcatgag cacggcatga aagagagcat agagacgttc ttcagggcac tatacgggct
     8041 ggccgagctc cagtcaacga ggaagaacag cgcaacttcc gacccgaaag aatgttccgc
     8101 cttcatatct cttatgaccg aactcggcag tcttgtaaaa gactgggata ctgtggcaga
     8161 ggatcttctc gaaagaaacg actacacggt tcagttgtgg acagacaaga agagtcgtgc
     8221 aaaggtcctg gtctgcctgc aggagatatg tccgggaaga tatttgccgg cgttctttgt
     8281 ccccgttccc gccggcgagg acatcaccga gattctcgat gatgttgctc ggaggatatc
     8341 ggccggggca atgcatgaag aacaggtctc cgcagcgccc taaactgctt tcgacccgaa
     8401 atgcttcgca ggggaagcac tccaatatgc agttcctaaa acatgagatt aagaaggctg
     8461 cttattttga gaaatgcttc cccgtatcct tttcaggaat tccttatcag ggaggtacat
     8521 atgtctcaag gacagcaaca gattgaaggg cattctgcaa agaccggtga aaggggaatg
     8581 aaacatacac ggaagcgcag ggcgaatgac gatactcctt taatcgatca catcatcgcg
     8641 gcaatgaagg agtataaggc tattacgata aagatagccg aagagttggg cttcgaacat
     8701 cggggagaac cttcatctga tgaagtgaaa aggtatcttc tcgatattcc catggatcct
     8761 gacgactatg cgcttctgca ccagatcggc ggaaagccca ccctctgcca cgccattctc
     8821 gaagctgtcg gcgcagccga ccatgaaata gaagaggcga gtggactcgc ggcgttcgcc
     8881 tacgacgcag tcggggtata ggacggtgag ctccagccgg aggtcaaaag gaggaattat
     8941 gacgctttcg aagatactcc ccagagaaat aaggatagct ctctacggag tggtcttgga
     9001 taccgataca aaaaagctta aagaaagagt acgggagatg aggcagatga ggcagagaag
     9061 gaaggcagtc ttgagcctgt gcagtcaatg ctggttcaag gactactgcc tcatacgcaa
     9121 tggtcttgat gagaagagaa cggcaattga gaaggccgct tgatcatctg tcgccgaacc
     9181 cttatgaaag gaggcactat gccaggcagc acggcattgc gtcaaaaaga gcgccggaaa
     9241 gtcatgcgaa aagaactcag ggagatagag aaaatgctcc atccctgtcc ggtcttcgat
     9301 ggcattgagg tgctccatga acacaggggc cgccgcatcg tcttcaccgc atggagagaa
     9361 gacacgcaaa tcaggggtaa agaagttgca tttccgagaa agtccgtgct ctacggaccg
     9421 gccgacttct atgaggcgaa caagagagac ttccaatggc ttgttgccgg gtcgtccggg
     9481 tagtctgact gatttcaggg agaggtaaaa accagttcct ctccctgaat gaccaaccaa
     9541 aaagaggaaa ccaagaatga taatcagtga gcacaaaggc gagaaaatag acgggcccgg
     9601 caaagtggca gacatcatcc gggcgctcct tgccgcagaa cacgaaaccg accgggagcg
     9661 ggagcatttc tgggccatcg ggctaaatac aaaaaatcgc ataaagtaca tcgaacttgt
     9721 cagtttaggc actctcagca attcgctgat tcacccccgc gagacatacc gactggccgt
     9781 aatgaacgcg gcggctcagt tgatcatagc tcacaaccat ccatccggcg atgtggcccc
     9841 gagccgcgac gacatagcaa taacggaacg tcttaaagca gcgggtgaga ttatggggat
     9901 cactcttctt gaccatgtga tattggggag cgacggcacg tttacaagta tgaaggaggc
     9961 gggccattta taggagttat gaagaccgaa gcgcagttac tcccaataat cttaaagaaa
    10021 ggaccaagca cagaggagaa cctatggaag actggagaca ttttctgctg tatgcgaaga
    10081 actactacct taaaacggag acaatatccg atctcttaaa gatattgtca cacgagacgg
    10141 ggctcgaccc aagaagcgtg aagctctggg acgtgtatga ggtcttggcc ggacgcttcg
    10201 tgaaatacac taggggagat cagaagaagg actttttcat gcggatgttc tcggaggggg
    10261 attccgtgaa gatcggcgat gtcgtcctgc agttgctcgc ggcattgagc gtccagaagg
    10321 tcagggatgg cgagaccgtt ctcattgatt tgggcgagcc cgatccgtct gtactgccgt
    10381 taaataaata ctgaaggaaa ggagatgcac atgtcttctg cgaatgcaat gaaggatctg
    10441 accgcgaggg tggaggaagg cataaaggtg acgaaagccg caattgcccg cggcgacgct
    10501 ttgcgtgtag gtgactgcct cagacacttc gccgccctca atgcccccat agacaaaata
    10561 tacgaagcca tcgaggagtg tccggtaagt ctcatagaag aagcgggata ttgcctaatc
    10621 gtgaagagtg cgctgctggg aagggaaatc atcctgggca aggatatccc gtggcaggaa
    10681 gtgatctctc ttctacaaaa gggtctaaca gggaaggacc tgatgactgt ccttgagact
    10741 cgtgagcttt tcgaggggaa ggttgtcctg acggattcac cgagcctgtt tgacacgccg
    10801 gcctgagaat cacatgacgg aaactcttag ggcaaaactc tggatgccgg aggtgaaaca
    10861 ggacgttacg aacaataaat taatccgaca gaaggggccg gaagagcttt gacgcttggc
    10921 ggcttcttct gtttttaaag gaggtgattg aatgaacaag acaatgaacg cttccgcaga
    10981 aacggggcaa ttcatagtgc ttgatctctt caacccagat atcgtcaatg ttgaatgtaa
    11041 tgaaaagggc gaaactctct actttgacac cgaggagaag gccatggagc acgcggaaga
    11101 cgtccaggat ccccttgtga tcgagctctc actcggaggc gcaaatgtag tgaggagcac
    11161 tcatgttctc ctcaatgttg ttctcttccc tgaaaacgag cagtcagatg acattccctc
    11221 cggaaaggag aaccacattt atctcaacgg aaagtatatc tccagcaggt gcaatgacga
    11281 atacgatgac tacacggtaa cgtgggaagg ttactgtggc atcttaaatg gcctcaagat
    11341 ggcgattccg ggactgactt gcaaggacat tctgatagat gatgtgagat tgactgaaat
    11401 cctcggcgaa aactggagag aatactatga ggaagggtcc ctatgggcaa gggttgagcg
    11461 gcaactcggc gtgcaggtta ttgaataata gaattggaaa ggcaaaaacc ttcgcaggga
    11521 tattgcgagc cttgcctccg attacgatcc gtttattgtc ctgaaagtga tattcacttg
    11581 tgctaaaata gtgaaaatga ataactcaaa ggagtaattt atgaatccac gtattatatc
    11641 gaatccaaat atttgcgggg gagagccgtg cattaagggg acgcgtattc cggttcacgt
    11701 aattctcagc catctcgcag caggtgaaga ttatgagacg atccttaaac atttcccgcg
    11761 aattaacaaa gaagatatcc ttgcgtgtct tgaatatgca tccttccttg ctacagagaa
    11821 aacagtagct gcatgaagat cattgtcgat gagagtgtga gctatggatt agtagcggtt
    11881 ttgagaacga ctgggcacaa ggttattgcc atagccgaat ctgcgacttc cggcacagtc
    11941 gatgaagaaa tttacaatct cgtcattcaa gaaggagcga ttcttatcag cagagacttt
    12001 catttcacca atgccttaag atttccgccc gaaagaacaa gaggaattat ttatattcgg
    12061 catggaaacc tgatggcgtt ggaagaaatc gagattgtcc agagatttct gtccgctcac
    12121 cggcacgatg aatataacgg tcgtttggca accctgtata aggacagtgt gaagattcgt
    12181 taagacgact cttttagatt tacctgcact ttctttgtct ggcctgagtt cgttgggaac
    12241 tctattttgg cggagtcgag attgttcacc ttgacatcat aagcgggctc gagatgacga
    12301 cttcgtacct cgcttctgga gggttctttt gatggtggtt atacgttgtc ttattcgatc
    12361 gacagtctct ccggagaaag tctctccgtc ggatgttatg gcgaggttta ctattccgaa
    12421 gtcgacgccc aaaatacctg tgggctcgaa ttttgctgca ttggtactct ccatacacaa
    12481 acagagatag aaggagccct ttttgtaaat gaggtccgcc tgtcctttaa taatagaaag
    12541 atcgaggatg atgccggggc gattcacaaa gtggatcgtc attcttccgt ccgtcgtcag
    12601 tatgctgacg gtgtctgcag ccttaaagga aaggatacgt tcgtcataga cgactgagga
    12661 atgttttcta aaaatatgca gccttttact ttcagccctg taggattcga ctaccttgcc
    12721 gattgctcga acggccagtt gagaggaaag gccatattca atgcgtacaa tccggtagca
    12781 aaggcgatgt agcaccgccc tgcggaaaga tttctccttc caagctatgc gagaaatatg
    12841 gttgcaggcc ctgttgaaga gctccgcagt cctcaacaat gactctttct gttcggcgga
    12901 actgagaagt tttgctttga ccgtcaatat cattaattag ttataagttg tcgagcttat
    12961 ttgt
//
LOCUS       DATKOU010000255         4983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3062417, whole genome shotgun sequence.
ACCESSION   DATKOU010000255 DATKOU010000000
VERSION     DATKOU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3062417"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VMU21_12230"
     CDS             <1..592
                     /locus_tag="VMU21_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUO78332.1"
                     /translation="PQNKKRTLLIDKELTQANIIIGHAGIRRENPDYYAVSVMNYILG
                     GGGFSSRLMQTVRDEMGLAYDIHSFFAPNKEGGLFEVGVQTKNESSKTVISEIYTQMN
                     RIKKDKVSDQELEDAKAYLTGSFPRKLDTNRKIADFLVAIEFYELGLDYVDKYPQYIR
                     AVTGDDVLRVAGKYLDTLGVVTVVVGKQSKISLDNQ"
     gene            592..1140
                     /locus_tag="VMU21_12235"
     CDS             592..1140
                     /locus_tag="VMU21_12235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUO78333.1"
                     /translation="MCVVCPSWLSFILYNPLRKIITNRQKVLEESGISHDSVVLEVGA
                     GNGFFTEVLAEHGKKIYSVELQRGMAGKLKKRVARFGYRVSMIECDIATCGIGDNVAD
                     VCLMYYSFHEVKDKAGAVRTISSALKEGGSLSVYEPTIEVNKKDMLNTARMFENAGFR
                     KEGERNGLFTRFVKLRRIGGGA"
     gene            1411..1483
                     /locus_tag="VMU21_12240"
     tRNA            1411..1483
                     /locus_tag="VMU21_12240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1444..1446,aa:Ala,seq:cgc)
     gene            complement(1493..1858)
                     /locus_tag="VMU21_12245"
     CDS             complement(1493..1858)
                     /locus_tag="VMU21_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78334.1"
                     /translation="MMRWFRIMVLSGFIVFITGGCASLAAVNKASETIKPSGIHEDCM
                     ELLPGQILEYSFEASKPLNFNVHYHEDHDVVYGITKDGVSGDKGTFRCEKKQYYCLMW
                     TNPGSEPVSLTYSYTTKKK"
     gene            2275..3540
                     /gene="leuC"
                     /locus_tag="VMU21_12250"
     CDS             2275..3540
                     /gene="leuC"
                     /locus_tag="VMU21_12250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HUO78335.1"
                     /translation="MTITEKILAAHAGKSEVVPGELINAKVDFILANDITAPIALQEF
                     KKIGAKDVFDRNRVAFIPDHFTPQKDIKAAEQCKQLKDFSKEYHLGLYFEVGRMGIEH
                     ALLPEQGLVVPGDVIIGADSHTCTYGALGAFATGVGSTDVGAAMATGECWFKVPESMK
                     FVYFGKLNKWVGGKDLILHTIGDIGVDGALYRSMEFEGEAIRSLPMYGRLTMCNMAIE
                     AGGKSGIIVPDNVTEEYVRGRAKRPYTMYTSDPGSRYVEIREYDCAKIPLTVSCPSLP
                     SNTKPAAELSHIAIDQVVIGSCTNGRLEDLREAARVIKGKKVNPNVRLIVIPATQRIY
                     KESMKEGLLEIFIDAEAVVSTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGHPES
                     EVYLSNPAVAAASAVLGRIGIPEEVIAHE"
     gene            3533..4417
                     /gene="hisG"
                     /locus_tag="VMU21_12255"
     CDS             3533..4417
                     /gene="hisG"
                     /locus_tag="VMU21_12255"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HUO78336.1"
                     /translation="MNNRKTILKLGLPKGSLQESTLRLFKKAGYHITVSARSYYPVID
                     DPEIEAMLIRAQEMARYVEDGVLDCGLTGHDWVLEQNADVVEVAELRYAKEGFRPVRW
                     VVAVPADSKIKSLRDLNGKRVATELVGFTKRYFRSKGIKAEVDFSWGATEVKPPYLAD
                     AIVEITETGTSLKANNLRIVESIIESTTRFIANKKAWHDKGKRQKMENIALLLKGALA
                     AEEKVGLKMNVPERSFKKVLSLLSAMHSPTVASLSDKGWYAIDVVINEKTVRDIIPKL
                     KSAGASGIVEYTLNKVIP"
     gene            complement(4640..4963)
                     /locus_tag="VMU21_12260"
     CDS             complement(4640..4963)
                     /locus_tag="VMU21_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78337.1"
                     /translation="MVSQDMRKVIMFPDVTTAYILRMKQLKKSPINPLWFISRVYPME
                     KRDTGKATREIIITKNPEMPSPEKSTSKTDEIGRGDKERGSPKQRKRPKTVKFRAPAA
                     ERIKE"
BASE COUNT         1269 a         1083 c         1378 g         1253 t
ORIGIN      
        1 gcctcaaaac aagaagagga ccctcctcat agacaaggaa ctgacccagg caaacatcat
       61 tatcggtcat gcggggatac gaagagaaaa tcctgactat tatgcggtct ctgtgatgaa
      121 ctatattctc ggaggaggcg gattttcttc caggctgatg cagacagtga gggacgagat
      181 ggggctcgcc tacgatattc atagtttttt cgctcccaat aaagaggggg ggctcttcga
      241 ggtgggggtc cagacaaaga acgaatcttc aaagacggtg atcagcgaga tttatacgca
      301 gatgaacaga ataaagaaag ataaggtctc tgatcaggaa ctggaggatg caaaggcata
      361 cctcacaggg agttttccca gaaaattgga tacaaacaga aagattgcgg acttccttgt
      421 tgccattgag ttttatgaac tcggcctgga ctatgtggac aaatatcccc agtatataag
      481 ggctgtgacc ggtgatgacg tgctgagggt tgccgggaaa tacctcgaca cgctgggtgt
      541 ggtgaccgtg gtggtgggaa aacagtcaaa gatttcctta gataaccagt aatgtgtgtt
      601 gtctgcccct cgtggctttc ctttatcctc tataatcctt taagaaagat aattactaac
      661 cggcaaaagg ttcttgagga atccgggatc agtcatgatt ccgtagtgct cgaggttggc
      721 gctggcaacg gattttttac tgaagttctt gcggaacatg ggaagaaaat ctattctgtt
      781 gaactgcagc gtggaatggc gggaaagctc aaaaagagag tggcgagatt cggctacagg
      841 gtgagcatga ttgaatgcga tatcgccaca tgcgggatcg gagataatgt cgcggacgtt
      901 tgcctcatgt attacagttt ccatgaggtg aaggacaagg ccggggccgt cagaacgatc
      961 agcagcgcgc ttaaggaggg agggtcactt tctgtctatg agccgacgat tgaggtgaat
     1021 aagaaagata tgctgaacac tgcgaggatg ttcgagaacg caggttttag aaaagagggg
     1081 gagaggaacg gccttttcac acgatttgtc aagctgagga ggattggtgg tggagcctag
     1141 cccacttcat ccttgagtgt ctttgtttct ggagaggcat ctgtctcccg gcaagacaat
     1201 agcccttctt gaatgtagtg gggctacaca cctaagaata ctctgttctt atgcattgtc
     1261 aaggtccgct cttgcgtaca cgcacataac cctgagtctt gcctttggag ccggacgcct
     1321 cagccgaatt tttcaattat caagcctaga atgatttatt tggaaaaatt tagtaaaata
     1381 gattgcctca gtcagtagaa gaccacgaga gggcggttag ctcagctggg agagcgccac
     1441 gttcgcaacg tggaagtcgg gggttcgaat cccctaccgt ccactttccc ctctatttct
     1501 tttttgtggt gtaagaatat gtgaggctga ccggctcaga acccgggttg gtccacataa
     1561 ggcaataata ttgctttttt tcacaacgga acgttccttt gtctccggag accccgtcct
     1621 ttgtgattcc atagacgaca tcatggtctt cgtggtagtg aacattgaag ttaagcggct
     1681 ttgaggcctc gaaggaatat tccaggattt gaccgggtag caactccata cagtcctcat
     1741 ggatgcccga tggtttaatc gtttcgctcg ctttattcac ggcagcgagg gaagcgcacc
     1801 ctccagtgat gaatacgata aaaccggaca ggaccataat acgaaaccat ctcatcatgt
     1861 cagcctcctt atgtacacaa aacatctttc ttatctatta tatatgatct cgtggcaata
     1921 gtcgctcatt gaacggactt tggcatagag cagacagcca ggaggagacg tatgaaactg
     1981 aagccgcagc ttatcattgg tttggcactg agccttttgt ccgcgtatta gtggggagcc
     2041 attggtaaag cggtgcctct cagcagcgtc cctttgttgt accaatatac tttccgcctt
     2101 tacccttatt ttgattactt catcaccgta gcggggatgt tccttttttg ccggggactg
     2161 aggaaactca gagagtggtg gcccttcgca ggggtaccgg gtaggaagtc ttgtgaatat
     2221 tgagcgagga gaggtataat aatttttcaa aaaatggtga gcagagggaa aacaatgacg
     2281 atcactgaaa agatacttgc agcgcatgcg gggaaatcag aggtagttcc tggggagctc
     2341 atcaatgcga aggtggattt catcctcgca aacgatatca ccgcgcctat cgctctccag
     2401 gagtttaaga agataggggc aaaggatgtg tttgacagga accgcgtggc ctttatcccc
     2461 gatcatttta cgccacaaaa ggatatcaaa gctgctgaac agtgcaagca actaaaggac
     2521 ttctcgaagg aataccatct tggactctat tttgaagtgg ggaggatggg cattgagcat
     2581 gcgctgttgc ctgaacaggg gctggtggtg cccggggatg tcattatagg ggctgacagc
     2641 catacatgca cctatggtgc gctgggcgct tttgcgaccg gggtggggtc taccgacgtg
     2701 ggcgcagcca tggcaacagg ggagtgctgg tttaaggtcc ctgagtcaat gaaatttgtc
     2761 tatttcggaa aactcaacaa atgggtcgga ggcaaggacc tgatactcca caccataggt
     2821 gatatcgggg ttgatggtgc cctctacagg agcatggaat ttgaaggtga ggccatacgc
     2881 agtctcccta tgtatggacg gctcactatg tgcaatatgg ccattgaggc aggaggcaaa
     2941 agcggtatta ttgttccgga caacgtaaca gaggagtacg tgcggggaag ggcaaagagg
     3001 ccctatacca tgtatacatc tgatcccggt tcgcggtatg tggaaataag agagtacgac
     3061 tgcgcgaaaa tccctttgac cgtgtcatgt ccttctctgc cctccaacac caagcctgct
     3121 gcagagctct cgcatatcgc catagatcag gtggtgatcg gctcatgcac caacggccgg
     3181 ctcgaggacc tgagagaggc tgcccgggtg ataaagggga aaaaggtgaa tcccaacgtg
     3241 cggttgattg tgataccggc aacgcagcgg atctacaaag agtcgatgaa agaggggctg
     3301 cttgagatat ttatcgatgc ggaggcggtg gtttccaccc ccacatgcgg gccgtgtctc
     3361 ggcgggcata tgggaattct cgcaaagggg gaaagggcgc tggcgaccac aaacagaaac
     3421 tttgtgggga gaatgggaca tcctgagagt gaagtgtatc tctcaaatcc cgcggtggcg
     3481 gcagcgagtg cggtgctagg gaggatcggg ataccggagg aggtaattgc tcatgaataa
     3541 cagaaagaca attctgaaac ttgggctccc aaaagggagc ctccaggaat caacgttgag
     3601 gcttttcaaa aaagccggat atcacattac cgtatccgcg aggtcgtatt acccggtcat
     3661 tgacgatccc gagatagagg ccatgctcat ccgggctcag gaaatggcgc ggtatgtgga
     3721 agacggtgtc ctggattgcg ggctgaccgg acatgactgg gtccttgaac agaatgcgga
     3781 tgtggtggag gttgctgaat tgaggtatgc gaaagagggt ttcagacccg ttagatgggt
     3841 tgttgctgtt cccgcggatt caaagataaa gagtctcagg gacctcaatg ggaaaagggt
     3901 tgcaacggaa cttgtagggt ttacgaaaag atatttcagg tccaagggga tcaaggcaga
     3961 ggtcgatttt tcatggggcg cgaccgaggt gaagcctcct tacctggctg atgccattgt
     4021 ggagatcacc gagacaggga cttcattaaa ggcgaacaac ctgaggattg tggagtcgat
     4081 cattgagtcc accacaagat ttattgccaa caaaaaagcg tggcatgata aagggaaaag
     4141 gcagaaaatg gaaaatatcg cattgctcct gaagggcgcc cttgccgcgg aagaaaaagt
     4201 tggcctcaag atgaatgtgc ctgagaggtc gttcaagaaa gtattaagtc tgctctctgc
     4261 catgcattca ccgacagtgg cttccctttc agataagggt tggtatgcca tcgatgtggt
     4321 gatcaacgaa aaaacagtga gagacataat cccgaaatta aagagcgcgg gagcttcagg
     4381 cattgtcgaa tacacgttga ataaagtaat accgtaactg accgaacctg cttacaatgc
     4441 cgggatatgt atccgcgtac gcgaaaacac aactccagac tgtcaaggct gtctttctct
     4501 ctgttgcgta aggctgtttg tctgtgataa ggtaaaagct gctttctatc atgtaccctg
     4561 aaaagtttct ctccataggt gtgctgtgtt tccttttggg gtcggttgtc tccctgctgc
     4621 cgcttcagag gacaaaaacc tactccttta tcctctctgc cgccggcgcc ctgaatttta
     4681 ccgtcttcgg tctcttcctc tgcttcgggc ttcccctttc cttgtctcct cttccgattt
     4741 catcggtctt tgaggtgctc ttttccgggg atggcatttc aggatttttt gtgattatta
     4801 tttctcttgt ggcctttccg gtatcccttt tctccatggg gtatacgcgc gaaatgaacc
     4861 acagggggtt gatgggactc ttcttcaatt gtttcattct tagtatgtac gctgtggtaa
     4921 catcagggaa cattatcacc tttctcatat cctgggagac catgtctgtt gtctcttatt
     4981 tcc
//
LOCUS       DATKOU010000256         7756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1147947, whole genome shotgun sequence.
ACCESSION   DATKOU010000256 DATKOU010000000
VERSION     DATKOU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147947"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VMU21_12265"
     CDS             <1..221
                     /locus_tag="VMU21_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78338.1"
                     /translation="EWSTKPLQLHTLPAPHLLDGLDRLRVVHAVEAERVDRGLQRQTS
                     HGRRDASNLLICAMPEYPAFMASHEILK"
     gene            393..1079
                     /locus_tag="VMU21_12270"
     CDS             393..1079
                     /locus_tag="VMU21_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HUO78339.1"
                     /translation="MLGEILKEKREGLGLDLKEVAHNLRIQYEYLKAIENNDLEKLPP
                     DVYTRAYIREYARFLNIDSGTLLDEYAKQQMSAHAVIQTEPPPVQKKFAFPRRVLLIP
                     LVIIIVVIFVIYFPRISPVKKTGEVPVAGTPSTVYEEAQVSPPHVSPVSTHNMLTVNA
                     VETTWLRIEIEEGMSEEVLMKPGETKQWTSTKGFSIKVGNAGGVRLVLNDKDMGIPGA
                     KGQVVKLTLP"
     gene            1148..1912
                     /gene="thiD"
                     /locus_tag="VMU21_12275"
     CDS             1148..1912
                     /gene="thiD"
                     /locus_tag="VMU21_12275"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HUO78340.1"
                     /translation="MKSVLTIAGSDPTGGAGLQADLKVFRAFGVHGLSVPAVLTAQNT
                     SGVGRLLSIDRDFFFAQIDLLLDDITPDAMKTGMLYSKWIVETVADRTRAHSLGNLVI
                     DPVTVSSSGTSLVEEGTLDRLRDLLFPLARVITPNIYEASVLTGMHVEDEKDMEKAAR
                     RLKEAGPEVVIITGGHLERLTLDLYFDGNTFHRLEGPKIKGEYHGTGCAFSAAIAACL
                     SLGYTPFESARRAKDFVTEAIKKSYHLGKGMGLLNV"
     gene            1934..3289
                     /gene="radA"
                     /locus_tag="VMU21_12280"
     CDS             1934..3289
                     /gene="radA"
                     /locus_tag="VMU21_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HUO78341.1"
                     /translation="MSKVKTFYQCQACGYSSPKWLGKCPDCGAWNTMVEEKSSPATRH
                     SAQAPLYKPQPLSSISGGKEQRVTTGMNELDRVLGGGIVDGSVILVGGDPGIGKSTLL
                     IQTGANFSERAGKVLYVSGEESPEQIKLRADRLSIHSDNVVLLPETNLEGIIQTAASL
                     SPGLIIIDSIQTMYTEELTSAPGSVGQVRECAAKLMSFAKRSQIPLFLIGHVTKEGAI
                     AGPRVLEHIVDTVLYFEGERGHAYRILRTVKNRFGSTNEIGVFQMTDSGLLPIENPSE
                     LFLSERPQNVSGSTVVASMEGTRPLLIELQALVSPTTFGMPRRTCIGVDFNRVNLLVA
                     VLEKIAGLHLGGMDIFVNVVGGIKIVEPAVDMGVVAAVASSLREIPIHPRTVLFGEVG
                     LSGEIRAVSQVDSRVKEAAKIGFERAILSKTNSARLKDSHGLEIIGASNVEEFLEVIF
                     G"
     gene            3282..3965
                     /locus_tag="VMU21_12285"
     CDS             3282..3965
                     /locus_tag="VMU21_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78342.1"
                     /translation="MAEHKGKNGVVLFLGIRTGRALSLSASFILCAVLSLLFTSCATK
                     KLEIPTYEGVDPKVILEEREHIKMLESTFSIEFEKEGSVVKGEGALRLTPDSLDLQVY
                     SFGFLVAEVSSNSTATSSNPPIDRNKLSLLVEGLRNSFFWWSVKNPDIRDDHGTYRVS
                     NSWKKVFIDKKTMMPLRQTIELDEGRQLDVKYEGPAFLDGVWFPSKMRIALSHQSVNL
                     TIETLSLKE"
     gene            complement(4098..4925)
                     /locus_tag="VMU21_12290"
     CDS             complement(4098..4925)
                     /locus_tag="VMU21_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO78343.1"
                     /translation="MDYLEYYGLKEHPFTNVVDNKFYYGSPQYRDALTKLKYAIDTKK
                     GLAVVVGEIGAGKTTLARRLLEELDENRYEATLLVIIHSSVSSEWLLRKFAIQLGVGD
                     VKEDSKLEMLGQIYRRLLEINQSGRTAVLLMDEVQMLKSREIMEEFRGLLNMEMPGGK
                     MINLIFFGLSDLEEVLALDEPLKQRVAMKISLKPFSVDDTRDYIKHRLRIAGCVHEVF
                     TDNAIREVYGYSTGLPRLINTICDNALLEGYLFKATTIDESVIRAVAIDLGLNGGKP"
     gene            complement(4928..7243)
                     /locus_tag="VMU21_12295"
     CDS             complement(4928..7243)
                     /locus_tag="VMU21_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO78344.1"
                     /translation="MADKSIIVKEAQKYLARGQIDKAIIEWEKLVKEAPDGNVYNTVG
                     DLYFKKGNKKSAVDFFHKAATFFREGGFSLKALALYKKVINIDPLDAGAFTALGELSE
                     EKGLVTDAIKYYLTAADILSRDANKERFVDIYERILSLAPSNFPLRDKVAGLFLKEGL
                     TSHAIKEYLHLARLCADKEDREQERLCFMKILDIQPDNKNALTGMSSVYEKKGDVKQA
                     IEFMKKAIALHHDDPHLSVRCALLYKESGAYDEAISYLSPVIQAKPTDTGAITLMGDI
                     HLARGDREKAWESYKTIVDSLTLESRLDEAIDLMKQFKDIDPVTIGKMLISLYRQKDD
                     MDTAFSEILIVADLLLDSGLQDEAVEYYREALAIHPDDMQIKRTLAELEMGALGVAPS
                     GTEGERSTEDLFTDADIFIKYGLHDEARAMLEGLKVKEPGNTETHRRLKSLYREMHDS
                     EQAITECLILAELYGRNGALEMRDAALREASEINPDDPRLLERASARSEEASPAQVSE
                     EPPSQSLDDYTEEIAEAEFYARQGLKDDALRIYHRLLALFPQESALREKILSLEGEIP
                     KPSIEGRGLSVEAGEWTRREDFVSPEAEIFEADELHEKGEPSLDTNVLDIFEEFKKGL
                     ERELEAEDFETHYNLGIAYKEMGLIDDAIKEFQTSKNDPKCSARSMTMLGICYMEKGL
                     FPLAIEAFKGALNTLTTRDESYWSAKYDLASAYEKNGNLKEAFQIFSEIYGVDSKFRQ
                     VNEKLDHLKPLLAKEEQTPQQKEKKDRVSYI"
     gene            complement(7332..7649)
                     /locus_tag="VMU21_12300"
     CDS             complement(7332..7649)
                     /locus_tag="VMU21_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78345.1"
                     /translation="MYDARFAQKKSNLRIGEIAKAKGWLSDDDVQRILIMQEDTLEKF
                     GDIAVRGKYLSAEHLEELIKEQEDTYIFFGEALVLIGAITEKDLIEQLKEFNKAKFFT
                     QDA"
BASE COUNT         1920 a         1869 c         1866 g         2101 t
ORIGIN      
        1 cagaatggag caccaagccc cttcagctcc acacgctgcc cgcgccgcat ctgctggacg
       61 gcctcgaccg cctgcgcgtc gtgcatgcag tcgaggcaga acgcgtcgac cgtggtctcc
      121 agcggcaaac ttcccacgga agaagggatg catcaaacct tcttatctgc gctatgccag
      181 aataccctgc ctttatggca tcccatgaaa tcctgaaata attacctaaa ggacttaccg
      241 atcctatgcc tgtcaccacc actctcttca tttgagatta tgataccata aggattatat
      301 ctcccgatgc gttgagtgga ctgccctttt cccttaattt atgctatatt ttttatgtag
      361 tgcatatgaa tctctgtgtc cgagtttgac aattgctggg agaaatttta aaggaaaaac
      421 gtgaagggtt ggggcttgac ctcaaagagg ttgcccataa tctgaggatt caatacgaat
      481 acctcaaggc catagaaaat aatgatctag aaaaactccc acccgatgtc tatactcggg
      541 cctacataag ggaatacgca agattcctca atattgactc cgggactctt ctcgatgagt
      601 atgcgaaaca acagatgagt gcgcatgcag tgattcagac tgagcctcct cccgtgcaga
      661 agaaatttgc tttccctcgg agagtgcttt taatacctct tgtcataatt attgtcgtca
      721 tctttgtcat ctatttcccc aggataagcc ctgtcaagaa aacaggtgag gttcccgtgg
      781 ccggaacccc ctcgactgtt tatgaggaag cgcaggtatc acctccccat gtaagtcccg
      841 tatcaacaca caacatgctc accgtgaacg ctgtagagac cacatggctt cggattgaaa
      901 tagaagaagg gatgtccgaa gaggtactca tgaagccagg ggaaacaaag cagtggacat
      961 ccacaaaggg attcagtatc aaggtgggaa atgcaggcgg ggtcaggttg gttttaaacg
     1021 acaaagatat gggcatccca ggagcaaagg ggcaggtagt aaagcttacg cttccctaac
     1081 aaattcttta tgttcataac ccgcagaaaa ggggcagaga ataacgcaga ggtgcgatag
     1141 ataagaaatg aaatcagttt tgaccattgc aggttctgat cccacagggg gtgccggact
     1201 gcaggcggac ctgaaggtct tcagggcctt cggagttcac ggattgtctg tccctgctgt
     1261 ccttaccgct cagaacacct ccggggtggg aagacttctt tccattgaca gggatttttt
     1321 ctttgcacag atagatcttc ttctcgatga tatcactcct gacgcaatga aaacaggaat
     1381 gctgtatagc aaatggattg tggaaacggt tgccgacagg acaagggctc attctctggg
     1441 caatctggtt attgacccag ttaccgtttc ttcatcaggg acgagtcttg tcgaagaggg
     1501 aactctcgac aggctaaggg atctcctttt tcccctcgca agagtcatca ccccgaacat
     1561 ttatgaggca tccgttctca cggggatgca cgttgaggat gaaaaggaca tggagaaggc
     1621 tgccaggagg ttaaaagagg caggtcccga ggtagtgatc atcaccggtg gacatctgga
     1681 acgattaacg ctggatcttt actttgacgg gaataccttt catagattag agggacccaa
     1741 gatcaaggga gagtatcatg gtacaggctg tgccttctca gcggccattg cagcttgtct
     1801 ctcattgggt tacactccct ttgaatcggc gagaagggca aaagatttcg tgaccgaagc
     1861 tattaagaag tcataccatc tgggaaaagg tatggggttg ctgaacgtat gagctccccg
     1921 ttgcgtatat gtgatgtcta aagtaaagac cttctatcaa tgtcaggctt gcggatacag
     1981 cagtccgaaa tggcttggca agtgtcctga ctgtggggca tggaacacca tggtggagga
     2041 gaaatcctct ccggccacac gtcattcagc acaagctccc ctttacaagc cccaacctct
     2101 cagttccata agcggaggga aagaacagag ggtaacgaca gggatgaatg aactggatag
     2161 ggtcctcggc ggcggtatcg tggatggttc ggtgatcctc gtggggggtg acccggggat
     2221 aggtaaatca accctcctta ttcagacagg ggcaaatttc tctgaaaggg cggggaaagt
     2281 gctttatgtg tcgggcgaag aatctcctga acagattaag ctcagggctg acaggctttc
     2341 catacattca gacaatgtcg ttcttctccc agagacaaac ctggagggga tcatacagac
     2401 tgccgcttca ctctcacccg gcttgattat catagactca attcagacaa tgtacacaga
     2461 ggaactcacc tcagcccctg gttctgtggg ccaggtgagg gagtgtgcag cgaaattgat
     2521 gtcctttgcg aagagatcgc agataccgct gtttcttata ggccatgtaa ctaaggaagg
     2581 tgcgattgcg gggccacgtg tccttgaaca tattgttgat acggtgcttt attttgaagg
     2641 agagagaggg catgcatacc ggatacttcg cacagtgaaa aataggtttg gttctacaaa
     2701 cgagattgga gtgttccaga tgacggattc cggcctcctg ccgatcgaaa atccttcgga
     2761 gcttttcctc tccgaaaggc cacagaatgt gtcgggttcc accgtggtgg cgagcatgga
     2821 aggaacacgg cctctgctaa tagaactcca ggccctggtc tccccaacca ccttcggcat
     2881 gccgaggagg acatgcatag gtgtggattt taacagggtc aatcttctgg tcgcggtgct
     2941 tgagaaaatc gcgggccttc atcttggcgg aatggatatc tttgtcaacg tagtgggggg
     3001 gatcaagatt gttgaacctg ctgttgacat gggcgttgta gcggccgtcg catcctctct
     3061 cagggagatt cccattcatc cccggaccgt gctttttgga gaggtcggat tatcagggga
     3121 aataagggcg gtgagccagg tggattccag ggttaaggag gccgcaaaga taggttttga
     3181 gagggcgata ctttcaaaaa caaatagtgc acggctgaag gatagccacg ggcttgaaat
     3241 cataggggcg agtaatgttg aggaatttct ggaggttatc tttggctgag cacaaaggga
     3301 aaaacggcgt ggttctattc ctgggcataa ggaccgggcg tgccctttct ttgtctgcct
     3361 cctttattct gtgtgcagtt ctttctctcc ttttcacatc atgtgccaca aaaaagctgg
     3421 agatccccac gtatgagggg gttgatccaa aagtaatact tgaggaaagg gaacatatca
     3481 aaatgcttga atctaccttc tccatagaat ttgagaaaga gggaagcgtt gtgaagggcg
     3541 agggggcgct caggcttaca cctgattccc tcgatctcca agtctattcc ttcggttttc
     3601 tcgtggcaga ggtctcctca aacagtacag caaccagcag caatcccccc attgacagaa
     3661 acaaactctc tctgctcgtg gaagggctta gaaatagctt tttctggtgg tctgtgaaga
     3721 accctgacat cagggatgat cacggcacgt accgtgtctc aaattcctgg aaaaaggttt
     3781 tcatcgataa gaaaaccatg atgcctttga ggcagacaat agagctggat gaaggaaggc
     3841 aattggatgt aaagtacgag ggaccggcgt tcctggatgg agtatggttt ccttcaaaaa
     3901 tgagaatagc actttcacac caatcggtca atctgacaat agagaccctt tcattaaaag
     3961 aataatgcaa ggactaaagt gtgaaattca ggaggataac ccctcgaatc gagtgcatga
     4021 atgtgttacc ccgaaatggc ttcaaggcct atgatatttt ggaaaaatgt cattcgggga
     4081 ttcccgggct cccttgtcta aggtttgccc ccatttaacc cgaggtcaat tgccactgcc
     4141 ctgatcacag actcatctat agtcgttgcc ttaaagaggt atccttcaag aagggcattg
     4201 tcacaaatgg tattaatgag ccttgggagt ccggtggagt atccgtacac ttcccttatg
     4261 gcattgtccg taaatacttc atggacgcat cctgcaatcc ttaatcgatg ctttatatag
     4321 tcccttgtat catctacgga aaacggcttg agcgaaatct tcatcgccac ccgctgtttc
     4381 aagggttcat ccagggccag gacttcttcc aggtcagaaa gtccgaaaaa gataaggttg
     4441 atcatttttc ctcctggcat ttccatatta aggaggcctc tgaactcctc cattatctcc
     4501 ctggacttga gcatctgaac ctcatccatg agcagcaccg cagttctgcc gctctgattg
     4561 atttccaata gccttctgta gatctgccca agcatctcca gcttgctatc ctctttcacg
     4621 tctccgacac cgagctgaat cgcaaatttc ctgagaagcc attctgagct cacggacgag
     4681 tgaataatga ccaggagggt cgcctcgtaa cggttctcat cgagctcttc aagaagcctc
     4741 cttgcaaggg tagtctttcc tgcgccaatt tcaccgacta ctaccgcaag gccctttttg
     4801 gtgtctattg cgtatttgag ttttgtgagg gcgtccctgt actgggggct cccgtaataa
     4861 aacttattgt ccaccacatt ggtaaacggg tgttccttca ggccgtaata ttcaagatag
     4921 tccatgttta gatataagaa actctgtcct ttttctcttt ctgttgcggt gtctgttctt
     4981 cctttgcgag gaggggcttg aggtggtcaa gcttctcatt gacctgcctg aattttgaat
     5041 ccacgccata tatctcgctg aatatctgga acgcctcttt caggttgcca tttttttcgt
     5101 aggcagatgc gagatcatac tttgcgctcc aatacgactc atcacgcgtg gtgagggtat
     5161 tcagagcccc tttaaatgcc tctatggcca ggggaaacag acctttttcc atataacata
     5221 tgccgagcat ggtcatggat ctggcggaac acttcggatc gttcttggac gtctgaaatt
     5281 cctttatggc gtcatctatc agccccatct ctttataggc gataccgagg ttatagtgcg
     5341 tctcaaaatc ctctgcctct aattccctct caagtccctt tttaaactcc tcgaagatat
     5401 cgagcacatt ggtatcaagg gacggttctc ccttctcgtg gagctcatcc gcttcaaaaa
     5461 tttctgcttc aggtgagaca aaatcctcgc gacgagtcca ttcgccggcc tcgacagaaa
     5521 gacctctgcc ctctatactt ggtttgggaa tttctccttc gagagataaa atcttttcgc
     5581 gcaatgcgct ctcctggggg aagagggcaa gaagtctatg gtatatcctt aaggcatcat
     5641 ccttcaggcc ctgcctcgca tagaattcgg cttcggcaat ttcctcagta taatcatcga
     5701 gactttgcga agggggctct tcagagactt gtgcaggtga agcctcttca ctcctcgctg
     5761 atgccctctc caggagtctg ggatcgtcag gatttatttc ggaggcctct ctaagggcag
     5821 cgtcacgcat ttccaatgcc ccatttctcc catacagttc agcgaggatc agacattctg
     5881 tgatcgcctg ttcactgtca tgcatttccc tataaagaga tttgagtctt cgatgcgtct
     5941 cggtgttgcc aggctctttt accttgaggc cttcgagcat tgcccgtgcc tcatcatgca
     6001 acccatattt tataaatatg tcagcatcag tgaagaggtc ttcagtcgac ctctcccctt
     6061 ccgtacccga gggtgctacc cccagcgctc ccatttccag ttctgcaagt gttctcttga
     6121 tctgcatgtc atccggatgt atggcgaggg cttccctata atactcgaca gcttcatcct
     6181 gaagcccgct gtccaaaagg aggtctgcta caataaggat ctcagaaaat gccgtatcca
     6241 tatcgtcttt ctgcctatat agggatatga gcattttccc tatggttaca ggatcgatat
     6301 ccttgaactg cttcatgagg tctatggcct catcaagtct gctctcaagg gtgagagaat
     6361 ccactattgt tttatacgat tcccaagcct tttccctgtc acctctggcg agatggatat
     6421 ctcccattag tgtgattgcc cccgtatctg tcggttttgc ctggatgacg ggtgagaggt
     6481 agctgatcgc ttcgtcgtag gcaccgctct ctttataaag gagggcgcat ctcactgata
     6541 aatgcggatc gtcgtggtgc aacgcgatcg cctttttcat gaattcaata gcctgtttca
     6601 catcgccctt tttctcatac acagaactca tgccggtcaa tgcattctta ttgtcaggtt
     6661 ggatatcaag aatcttcata aaacataaac gctcctgctc ccggtcctcc ttgtcagcgc
     6721 aaaggcgggc aagatggaga tattccttta tggcatgtga ggtaaggcct tccttgagaa
     6781 acagtccggc gaccttgtcc ctcaagggga aattagatgg ggcgagcgaa agtattctct
     6841 catagatgtc cacaaatctc tctttgtttg cgtccctcga gaggatatcc gctgcagtga
     6901 gatagtattt tatcgcatcg gtgacaaggc ccttttcctc gcttaattcg cccaaagcag
     6961 taaaggctcc tgcgtctaag gggtcaatat taataacttt tttgtaaagg gcaagggcct
     7021 tgagcgaaaa acctccttct ctgaaaaagg ttgcggcctt atggaagaaa tccaccgcag
     7081 atttcttgtt ccctttttta aagtagagat ctcctacggt attgtaaaca ttaccgtccg
     7141 gggcctcttt gacaagtttt tcccattcaa tgatggcctt gtcaatctgg cctctcgcaa
     7201 gatatttttg ggcctctttt acaattatgc ttttgtctgc catgggctat tactgctact
     7261 tgaaaagtat catacatcag taaaaggtgt caataagcaa agattttttg ctgtgcgcat
     7321 tagataaggg attatgcatc ctgcgtgaaa aacttagcct tattgaattc cttgagttgt
     7381 tcgatgagat ccttttcggt gatcgctcca atcagaacca gggcctctcc aaaaaagata
     7441 taggtgtctt cctgttcctt gatgagctcc tctaggtgct ccgccgaaag atacttgccc
     7501 cttaccgcaa tatcgccaaa tttctctaat gtgtcttctt gcataatgag tatcctctgg
     7561 acgtcgtcgt cgctgagcca gccttttgcc tttgcgatct ccccaattct caggttactt
     7621 ttcttctggg caaatctcgc atcatacatg tcctgttctg atatcaatcc tttctgaagg
     7681 aggaagaggc caaagtttaa tacatttctt cccatgagaa aattatatca catgagagaa
     7741 aacactttgg caggag
//
LOCUS       DATKOU010000257        10002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4980992, whole genome shotgun sequence.
ACCESSION   DATKOU010000257 DATKOU010000000
VERSION     DATKOU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4980992"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..2033)
                     /gene="metG"
                     /locus_tag="VMU21_12305"
     CDS             complement(51..2033)
                     /gene="metG"
                     /locus_tag="VMU21_12305"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HUO78346.1"
                     /translation="MKENFFVTTPIYYVNDVPHIGHAYTTIAADILARRNRLLGKQVF
                     FLTGTDEHGQKVEKAAAEKGRTPKEHADIMVENFKGLWRKLNVSLDAFIRTTDTEHMK
                     TVQGLMQMLWDRGEIEKRSYSGWYCTPDERFWTEKELVDGSCPDCGRPVEQIHEENYF
                     FLMSKYQKSLIDYIEGNPSYILPETRRNEVMGFLKNNTLGDLCISRPVQRLSWGIPLP
                     FDADFVTYVWFDALVNYFSATRYLAPDAHKARTGEFWWPASHHIIGKDILITHAIYWS
                     TMLMALNLPLPENIFAHGWWTVEGRKMSKSLGNVVDPHAMVERYGVDAFRYFLFREVT
                     FGLDGDFSEEAFVRRINTDLANDLGNLLSRFLTMAEKYFEGRIHVPSQGESTHPLNDF
                     GAECVRALTSAYDEGKWSRLNFNTILEHLWSVVEAANNHIAQKEPWKLAKNNPDELKE
                     VLMNIWNALRLAALSLYPFMPGTAEEMWRHLGLKSLTDEVDQSFSSPQAGGGRYPMIF
                     TWAWKPAYEIRVFKGNQLFPRIETGGKPRMETASVGREEKEKSTDNYIPIEDFAKVEL
                     KIGRVVAAERVERSDKLIKLRVDIGEERQIVAGIGKAYDPEYLVGKEIVVVANLKPAK
                     LMGVESRGMLLAASDEDGKPSILILDREVKPGTRVK"
     gene            complement(2030..3025)
                     /locus_tag="VMU21_12310"
     CDS             complement(2030..3025)
                     /locus_tag="VMU21_12310"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HUO78347.1"
                     /translation="MSDVVGIRFKTCGKIYDFEVNGLEVKRGEAVVVESELGLSIGNV
                     AVERHTLESAAKELKKVLRKATEDDFKQKTDNEGLEGEARAYCLERIMARGLPMKLVC
                     TEVTLDRKRIIFYFAADGRIDFRELVKDLAAKFKTRIEMRQIGVRDEAKIVGGLGICG
                     RELCCRTFLTSFEPISIKMAKSQELVLNANKLSGVCGRLMCCLSYEYSENGEGCPRCK
                     EPEVAAGTDEKTVLEGAESPSEPVEEAISLSAEPSGEERRSRSARPFQSDVLSARRDT
                     YDQRRGEITPPMGGDAVKTEQGQGAGEGAGPEQKTPEGEKKFHRRKRRHRRRFHK"
     gene            complement(3065..4165)
                     /locus_tag="VMU21_12315"
     CDS             complement(3065..4165)
                     /locus_tag="VMU21_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HUO78348.1"
                     /translation="MALKDVMGQGRAVGIILGTLRNNRVPSAYLFTGESGIGKKFAAI
                     NLAKAVNCLQRAIGSQSLFITQENSGESSEGIPVSGDIPIDACDECSTCRKIESFTHP
                     DLLVISPEKGEIRVSEIRAVEDVLSLKPYEGRRKVVIIDDAHTMNQSAANAFLKTLEE
                     PPEESLLVLVTPHPDMLAETIRSRCSRIAFTPLSPDMCEEVIKKVHSKDSKRSEDISG
                     LPTMVRLCMGRPGLAISSDRVRERERCITLLENMAGGDGETWADREEMEQWLDMACIL
                     LRDMALTKIVQESPEEGALSPTDARNVLLNADIQEFISEMTTPNAVKDILGAYEKLIV
                     LKGVLGFNLNKAVTWNYVSSIMQNLVLLRRTR"
     gene            complement(4489..5049)
                     /locus_tag="VMU21_12320"
     CDS             complement(4489..5049)
                     /locus_tag="VMU21_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HUO78349.1"
                     /translation="MDKGRKLVSSVCILAMVCAVSLSGGCETKAQTGALSGAGAGALI
                     GGLANMHGSWGATALLGAGIGAGVGYLIGNEADKKDAAKRQAARKEELRPLAGTTWQV
                     VSITPKPEKSFKSTVSHFRTDGTVVTTKTNMDGKVLKETENYRVVGSTLILSKPDYVV
                     NAKFIIKGNKLTIDYGNGSVVMKRVS"
     gene            5285..6349
                     /gene="corA"
                     /locus_tag="VMU21_12325"
     CDS             5285..6349
                     /gene="corA"
                     /locus_tag="VMU21_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HUO78350.1"
                     /translation="MKGHITKRSKKSGLPPGTLVHIGEKKADASRITVLDYNEANFQE
                     AEFSTIEECFLFKDKPSVTWINVEGLHQVEIVQKLGDCYGFHPLVLEDILNTQQRPKI
                     EDYGDYFYVVLKMLHNEKSPEIVAEQISIILGQNYVISFQEGMEGDVFDPIRERLRSK
                     KGRIRGMGADYLAYSLIDAIVDNYFVILEKMGERIEDLEEELVKVPTTETLQGIHHLK
                     REMIFLRKAVWPLREVINSAGKGESPLIKDSTRIYLRDVYDHTIQVIDTIESGRDMLS
                     GMLDIYLSSLSNRMNEIMKFLTVIGTIFIPLTFIAGVYGMNFEFMPELKWRWGYFVLL
                     GVMGGIGIGMILYFKKKNWL"
     gene            complement(6419..7462)
                     /gene="selD"
                     /locus_tag="VMU21_12330"
     CDS             complement(6419..7462)
                     /gene="selD"
                     /locus_tag="VMU21_12330"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7421..7423),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HUO78351.1"
                     /translation="MEKRLTELSSCAGUASKFSPSDLSYVLGQLPAVTDKNVLVGLSN
                     ADDAGVYRITDDIAVVFTADYFTPIVDDPYWFGAIAAANSLSDVYAMGGKPLVALNLA
                     SFPGEAEFLPYAKRIMQGGVDKMTEAGVSIIGGHTIKDKEPKYGFAVLGRIHPDRIYD
                     NSKAKRGDSLILTKPIGTGVIATGIKAGMCSDARIEEITKSMATLNKRACEIMTEVGV
                     STATDITGFGLIGHLNEVLTASKCRARLNSKRVPFFKEAIRVADMGKIPAGTRSNEKT
                     YEPFVEWSAEVADTKRILMNDAQTSGGLLIFVPGERRAKLTTALESAGILAAYIGDIV
                     DEGVKDARSIFVD"
     gene            complement(7528..7797)
                     /locus_tag="VMU21_12335"
     CDS             complement(7528..7797)
                     /locus_tag="VMU21_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78352.1"
                     /translation="MIIRNEDVKGLMVEVPEGHTHLRVSLVLHDGSEMVFQEATVANL
                     VRAYITLKTHPRKNRVRLKGKRLDQRKEGFAEWQLLGAEGEDETA"
     gene            8052..>10002
                     /locus_tag="VMU21_12340"
     CDS             8052..>10002
                     /locus_tag="VMU21_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78353.1"
                     /translation="MAYGRVLAAIFLFSFTSLAFEIALTRLFSISLWYHFGFMVISIA
                     MLGVGASGTVLSLFPKLKNLSSDKPISHTIGMYGLLLGSTMALSYVISNILPFDPVRL
                     PWDKTQILYILLYYLILSTPFFFFGLTISSAFSALSERAGLLYGADLLGAGTGSLGFL
                     MLTARMSTEHAVVILSSMALAGAFITGKGKTRIAAVLFLIVNAFLFSMPDLITPRMSA
                     YKGLQSALNYPGAVYLRTYNDPFSRVDIFESPAVRFAPGLSLTYLDSLPRQIGLSIDG
                     SDINAVTDAGSTESLRFLSYLPSALPYVMARRDDVLILEPKGGLHVVLAGYYGAQNIY
                     KVDSNPLVIKVIKDTLGDFSQGIYESNTRDGLGRAWLKAKKTKFDLIDMPLMGASPSG
                     SFGIAEDYRFTVEAFKEYLGHLKETGILSIHLFILPPPRVELRLLSTLATSMEEMGIK
                     EVDKRIISFRSWGTVSILARTVPFTPQEIETVRQFSRQRRFDLIHLPGIREEETNNYV
                     RTPSNEYFLAFKQILDPETRKSFEDNYLFDIRPVRDENPFFHYYLRITNLEKIYRIMG
                     RKWQYFIEEGYLLPAVFVQVLLLSILLISLPAISKLKAKVEVKMKKTNKPILNIDLFY
                     FAFLGLGFMFVEIALIQKMILPLENP"
BASE COUNT         2383 a         2702 c         2204 g         2713 t
ORIGIN      
        1 gtgtttgtta atggtacagg tttccggagc attcctgaaa aagagtctca ttacttcacc
       61 cttgttcccg gcttcacttc cctgtccaga atgaggattg atggctttcc gtcttcgtca
      121 ctggctgcca ggagcattcc ccgggactcc acccccatga gcttcgcggg cttgaggttc
      181 gctaccacta ctatctcctt acccacaaga tattcagggt catatgcctt tccaatccct
      241 gcaacgatct gcctttcctc tccgatatcg actctgagct ttattagctt atcggatctt
      301 tcgacccttt ccgcggccac taccctgcca atcttaagtt ctacctttgc aaaatcctct
      361 atcgggatat aattgtctgt ggatttctcc ttctcttccc tgccgactga agctgtctcc
      421 attcttggtt ttcctcctgt ttcgatccta gggaaaagct ggttcccctt aaagaccctg
      481 atttcatacg ccggcttcca tgcccaggta aaaatcatgg gatatcgtcc accaccagcc
      541 tgcggcgagg aaaaactttg gtcaacctcg tcagtcaatg atttcagtcc cagatgtctc
      601 cacatctcct ccgcggtacc cggcatgaaa gggtaaaggg aaagggcggc gagtctgagg
      661 gcattccaga tattcatcag aacttccttg agctcatcag gattattctt ggcaagtttc
      721 catggttctt tctgggcaat atggttattt gcagcctcga cgacactcca caggtgttcc
      781 agtatggtat taaaattcag gcgtgaccat ttcccttcat cataggcgct ggtgagtgcc
      841 cttacgcatt cagccccaaa atcattcaaa ggatgggtgc tctctccctg ggaaggcaca
      901 tgaattctac cctcaaaata cttctctgcc atagtaagga accgggagag gaggtttcca
      961 aggtcattgg cgaggtcagt gttgatacgc cgaacaaagg cctcttcaga aaaatccccg
     1021 tccagaccga aggtcacttc cctgaacaag aagtatctga atgcgtccac tccatatctc
     1081 tcaaccatag catgggggtc caccacattt cccagggatt tggacatctt tctcccctct
     1141 acagtccacc acccgtgggc gaagatattt tcgggaagag gcaggttaag tgccatgagc
     1201 attgtggacc agtagatggc atgggtgata aggatgtcct tgccgatgat gtggtgggat
     1261 gcaggccacc agaattcacc tgtccttgcc ttgtgcgcat caggagcaag gtaacgcgtg
     1321 gcggagaaat agttcacgag ggcgtcaaac cagacatagg tgacaaagtc tgcatcaaaa
     1381 gggagaggaa ttccccagga gagcctttgc actggtctcg aaatgcagag gtctccaagg
     1441 gtgttgttct tgagaaatcc catgacctca tttctgcggg tctcgggaag tatatatgag
     1501 gggttgccct caatatagtc aatgagtgat ttctgatatt ttgacatgag gaaaaaatag
     1561 ttttcttcgt gaatctgctc aacgggcctt ccgcagtccg gacagctgcc gtcaaccagt
     1621 tccttttctg tccagaatct ctcgtcaggg gtgcaatacc agccggagta agaccttttc
     1681 tctatctctc ccctgtccca gagcatttgc ataagcccct gaactgtctt catgtgctcg
     1741 gtatcggtag tacggatgaa ggcatcgagg gagacattga gttttctcca tagccccttg
     1801 aagttttcca ccattatatc ggcatgctct tttggtgttc gccctttttc cgcagcagct
     1861 ttctcgacct tctgcccatg ttcatcagtg cctgtgagga aaaaaacctg tttcccaagc
     1921 agcctgttac gcctcgcgag gatgtctgct gcaatggtgg tatatgcatg cccgatatgg
     1981 ggaacgtcgt ttacgtaata aataggtgta gtgacaaaaa agttttcttt catttatgga
     2041 atcttctcct gtgtcttctt ttgcgtctat ggaatttttt ctcgccttcc ggggttttct
     2101 gctcagggcc tgcaccttcc cctgctcctt gcccttgttc agtcttaacc gcatcccctc
     2161 ccatgggtgg ggttatttct ccacgtctct ggtcataggt gtcccttcgt gcagaaagga
     2221 catctgactg aaacggtctg gcactcctcg atcttctttc ctcaccggaa ggttcagcac
     2281 tcaaagaaat ggcttcctcg accggttcag aaggactttc tgcaccctca agcacagtct
     2341 tctcatctgt tcctgcggca acctcaggct ccttacaccg gggacatccc tccccatttt
     2401 cactatattc atagctcaga caacacataa gtctcccaca gacaccagaa agcttattgg
     2461 cattcaggac aagctcctgg ctttttgcca tcttgatgga tatgggttca aatgaggtga
     2521 gaaacgtcct gcagcacaac tcccttccgc atatccccag tccacccaca atcttcgcct
     2581 cgtcccttac cccgatctgc ctcatctcta tccgcgtttt gaattttgcc gcaagatcct
     2641 tcacaagctc cctgaaatct atcctgccat ccgcagcaaa ataaaagatg atacgcttcc
     2701 tgtccagggt cacctccgta cataccagtt tcatagggag gcccctcgcc attatccttt
     2761 cgagacagta cgccctggct tctccttcaa gaccttcatt atcagtcttc tgtttaaagt
     2821 cgtcttccgt ggcttttctc aggaccttct tcagctcttt cgcagcactt tcgagagtgt
     2881 gcctttccac cgccacatta ccaatgctca atcccagttc agattccacc acaacggctt
     2941 ccccacgttt cacctcaagg ccattcacct caaagtcgta tatcttgcca catgtcttaa
     3001 accttatccc taccacgtca gacatccagc ctccggaaat ccagcaaagt caagaggcgc
     3061 atcctcatcg cgtccttctc agcagaacca gattttgcat tatgctcgat acatagttcc
     3121 aggtaactgc cttgttcaga ttgaaaccca aaaccccttt cagaacgatg agcttctcat
     3181 atgcgcccag tatatccttt accgcattcg gtgttgtcat ttcagaaata aactcctgaa
     3241 tatcggcgtt gagcaatacg tttcttgcat ctgtagggga aagagcgcct tcttcaggag
     3301 actcttgcac aatcttcgtg agcgccatat ccctcaacaa aatacatgcc atgtcaagcc
     3361 actgctccat ttcctcacgg tctgcccatg tttcaccatc tcccccagcc atgttctcga
     3421 gaagggtaat gcatcgctcc ctctccctca ctctgtcaga ggaaatggca agacccggcc
     3481 ttcccatgca gagcctaacc atcgttggca atccggatat atcctcagat ctcttactat
     3541 ccttgctgtg gactttcttt atgacctcct cgcacatatc aggtgaaagg ggcgtaaagg
     3601 ctattcggga gcaacgggag cgtatggtct ctgccagcat gtccggatgc ggcgttacca
     3661 atacgagcag gctttcttca ggaggctctt caagggtctt aagaaaggca tttgcagcgg
     3721 actggttcat ggtatgggca tcatcaatga tcacaacctt tcttctcccc tcataaggtt
     3781 ttaatgagag gacatcttcc acagccctta tctcgctcac gcggatctct cccttttccg
     3841 gagaaatcac caggaggtca ggatgtgtga acgactcaat ctttctgcag gtactgcatt
     3901 cgtcgcaggc gtctattggt atatccccgg aaaccggaat cccttcagat gattctccgc
     3961 tgttttcctg ggtgatgaac aatgactggg aaccaatggc tctttgcagg cagtttaccg
     4021 cctttgcaag gttaatggca gcaaatttct ttccgatccc cgactctcct gtaaaaagat
     4081 atgcggaggg cactcggttg ttcctgaggg tcccgaggat gatacccact gccctgccct
     4141 gtcccataac atccttaagt gccataacct attttaccat aagacaaatt ttaattatgg
     4201 agaaccctgt ggcagcaggc aggtaaaaac gctttccttt catttcattt cacaatcttt
     4261 tttcaatatt cttcaaccgg agaataagga aagttcttgc acaacctctc ctgtttaggg
     4321 atacggttgt gctcctgaca aagcataagg cgaaagcgat gctgcctctc cgcccttctg
     4381 ctggtgagca ttagccttat aggaccactc cccggcttca gcccggagag cggtcctatt
     4441 gttttcataa ccgtttcaag acatggggtg gattggtgtg gagaatcatc agctgacacg
     4501 tttcatgaca acgcttccat ttccgtaatc tatggttagc ttattccctt tgatgatgaa
     4561 ctttgcgttc accacatagt caggcttaga aaggatgagc gtagatccta ccacacggta
     4621 gttttctgtt tctttgagta ctttcccatc catatttgtc tttgtggtca cgacagttcc
     4681 atccgtcctg aaatgcgaca cagttgactt aaacgacttc tctggtttag gtgtaatgct
     4741 gaccacctgc caggtagtgc ctgccaaagg tctgagctct tctttacgcg ctgcctgacg
     4801 cttcgctgca tccttcttgt ccgcttcatt cccaatgaga taccccactc cggctccaat
     4861 gcccgctccc agcaaggctg ttgcccccca ggatccatgc atgttcgcaa gcccgccaat
     4921 gagggcgccc gctcccgcac cactgagtgc gcctgtctgg gcttttgttt cgcaaccacc
     4981 actcagtgat accgcacaca ccattgccaa aatacatacg ctactcacta atttcctgcc
     5041 cttgtccatt ccctcctcct ttcttatagt tctttttctg atcccttaca atgaagggct
     5101 gaaaagacta ccctcatagt catttgtatc cattatacct cgtttcagcc tgtaggaaaa
     5161 ctgccaggga atgtctcggc cttgccccat gggaaaactg agtgaagctt ttgaggctgt
     5221 gaatccccgt tgtcttggaa gcagtgatgt gatacaatgc gtgcagcgcg ggaggtgctt
     5281 cagtatgaaa ggacatatca ccaaaaggtc aaagaagagc ggtcttcccc ctgggaccct
     5341 tgttcatatt ggtgaaaaga aggcggatgc aagcaggatc acggtcctgg attacaatga
     5401 agcgaatttt caggaagcgg aattctccac gattgaggag tgttttctct ttaaagacaa
     5461 accctctgta acgtggatca atgtcgaagg ccttcatcag gtcgaaatcg tacagaagct
     5521 tggggattgc tatggctttc acccccttgt gctggaagac atccttaaca cccagcagcg
     5581 gccgaagata gaggattacg gagactactt ctatgtcgta ctcaagatgc tccacaacga
     5641 aaagagccct gagattgttg cggagcagat aagtatcatc ctcggccaga attatgtgat
     5701 ctccttccag gagggcatgg aaggggatgt ctttgacccc ataagggaga ggctaaggag
     5761 caaaaaaggc cgcatcaggg gtatgggggc cgattacctt gcctattcct tgatagacgc
     5821 catcgttgac aattactttg tgatcctcga aaaaatgggg gaaaggattg aagatctgga
     5881 agaagaactg gtgaaggtcc cgaccacaga aacccttcag gggatccacc atctgaaaag
     5941 agagatgatc ttcttgcgaa aggctgtctg gcccctccgg gaagtaatta atagcgcagg
     6001 gaaaggggag tcccctctga tcaaggattc caccaggatc tatttgaggg atgtctatga
     6061 ccataccatc caggtgattg ataccattga gtcgggcagg gatatgctct cgggcatgct
     6121 cgatatctac ctgtcaagct tgagcaacag gatgaatgag ataatgaaat tcctcaccgt
     6181 catcggcacc atattcatac ccctgacctt cattgcaggg gtgtacggga tgaattttga
     6241 gtttatgcct gaactcaaat ggcggtgggg ctatttcgtt ttgctcgggg tcatgggtgg
     6301 gataggcatt ggcatgatcc tctacttcaa aaagaaaaat tggctttaac cctgacagat
     6361 tcgaaagagg tatccggctc caatggcaag aagaggaatt gagagaaggt aagcggcact
     6421 aatccacaaa aatactcctt gcgtccttca ccccttcatc cacaatatca cctatatatg
     6481 ctgcaaggat acctgcgctc tccagggctg tggtgagttt tgcccgtctc tctcccggca
     6541 caaagatgag aagaccccct gatgtctggg catcgttcat caggattctt ttggtatccg
     6601 ccacctcagc agaccattcc acaaagggtt catacgtctt ctcgttactc ctcgtacctg
     6661 caggtatttt ccccatgtcg gcaaccctta tcgcctcttt gaaaaacggc acgcgttttg
     6721 aattaagacg agcccggcat ttactcgcag tgagcacctc attgagatga ccgatgaggc
     6781 cgaatccggt tatgtctgtt gcagtgctca ctcccacttc agtcataatc tcacaggccc
     6841 tcttattcag agttgccatg gacttggtaa tctcttcaat tctggcatcg ctacacatgc
     6901 cggcctttat cccggtagcg atcactccgg tcccaatggg ttttgtgagt atcagggaat
     6961 cccctctctt tgcctttgaa ttatcataaa tcctgtcagg gtggattctc ccgagaacgg
     7021 caaatccata cttgggctcc ttatccttaa tggtatgtcc cccgataatg gatacccctg
     7081 cttcggtcat tttatcgaca cctccctgca ttatcctctt cgcgtaagga aggaactcgg
     7141 cctcaccagg aaatgaggca aggttcagtg caaccaacgg cttacctccc atggcgtaca
     7201 cgtccgagag tgaattagcc gcggcaatag caccgaacca atagggatcg tccactatag
     7261 gggtaaaata atccgcggta aatacaacgg cgatatcgtc agtgatacgg tatacccctg
     7321 cgtcatcagc attggacaaa cccaccagca cattcttgtc ggtcacggca ggcagctgtc
     7381 ccagcacgta ggacaggtcc gaaggactga attttgatgc tcaacccgcg caacttgaaa
     7441 gttcggtcag cctcttttcc attgttctcc tttcctgctc gtttctataa aaaatattag
     7501 taagtttccc tgtatttgta aagctgttta tgcggtctca tcctcccctt cagccccaag
     7561 gagctgccac tcggcaaaac cttctttcct ctggtcaagt cttttccctt tcagtctcac
     7621 cctgttcttg cgaggatgtg tcttgagcgt gatatatgcc cttacaaggt ttgccacagt
     7681 ggcctcctga aagaccattt cagaaccatc atgaaggaca agggataccc tcagatgcgt
     7741 gtggccctca ggcacctcaa ccattaaccc tttcacatcc tcattcctta ttatcatcca
     7801 cagtcctcct ataaaggatc ctcagcctat tatagcccca catgcacttt tttgccctcc
     7861 agcccgttct cttcatgggt tttctttctt ataggagagg catcagccgg atacctctcc
     7921 tgtgtttatg aattatccag gccagagcac cgccaaaggc ttgacaacac cccacacata
     7981 aggtaaaaga aaattatgaa gagattacca acccttgctg gtaagggtta gagaaccgct
     8041 cactgggccc aatggcatat ggaagagtac ttgccgcgat ttttctcttt tccttcacat
     8101 cccttgcatt tgagatagcc ctcacaagac tcttttccat ctccctctgg tatcatttcg
     8161 ggtttatggt cataagtatt gcgatgctcg gcgtcggtgc cagcgggacg gtcctttcgc
     8221 tctttccgaa actcaagaat ctatcgtcgg acaagcctat ttcccatacg atcgggatgt
     8281 acggtctact cctgggctca accatggcat taagttatgt aatatccaat atattacctt
     8341 ttgaccctgt gaggcttcca tgggacaaga cccagatact gtacattttg ctctattacc
     8401 tcatcctctc cacgccattt tttttcttcg ggctgaccat ttccagcgca ttctctgccc
     8461 tcagcgaaag ggccgggctc ctctacgggg ctgatctcct tggtgccggg acaggctccc
     8521 tcggcttcct tatgctcaca gccagaatga gcaccgaaca tgctgttgtt attttgtcgt
     8581 ccatggcact cgcaggtgct ttcataacag gcaaaggaaa gacaagaatc gcagcagtgc
     8641 tgtttcttat agtgaatgca ttcctcttca gcatgcctga cctcatcacc ccaagaatgt
     8701 cagcctacaa aggccttcag tcagccctca actaccctgg agcagtatac ctaaggacat
     8761 ataatgaccc cttttcccgc gtcgatatat ttgaaagtcc ggcagtgagg tttgcgcctg
     8821 gtctcagcct cacctacctc gattctctcc ccaggcaaat aggtctttcc atagatggaa
     8881 gcgacatcaa tgcagtcacc gatgcgggca gcaccgaatc actcaggttc ctctcctatt
     8941 tgccctctgc ccttccctat gtcatggcga gaagagatga tgtcctcatc ctcgaaccaa
     9001 aaggcggact tcacgtagta ttggccgggt attatggtgc acaaaatatt tataaagtag
     9061 actccaatcc cctcgtcatc aaggtgataa aggataccct gggagatttt tcccaaggca
     9121 tctatgagag caatacaagg gacggtctgg ggagggcctg gttgaaggcg aaaaaaacca
     9181 aattcgacct tatcgatatg cccttgatgg gagcctcacc atccggctct tttgggatcg
     9241 ctgaggatta tcgcttcacc gtagaggcat tcaaggaata tctgggccat ctaaaggaaa
     9301 caggaatctt gagtattcat cttttcatcc tccctccgcc acgggtagaa ctgagactcc
     9361 tcagcactct cgccacatcc atggaagaga tggggataaa ggaagtggac aagaggataa
     9421 tctccttcag gagctggggc acggtgagca ttcttgccag gacagtgccg ttcacccctc
     9481 aagaaataga aaccgtcaga cagttctcaa ggcaaaggag gttcgacctt attcaccttc
     9541 cgggcataag ggaagaggag acgaataact acgtgagaac accttccaat gaatactttc
     9601 tcgcatttaa gcaaattctt gatccggaaa cgcgtaaatc ctttgaggat aattacctct
     9661 ttgatatccg tccggtgcgg gatgaaaatc cgttcttcca ttattatctg aggatcacga
     9721 acctcgaaaa gatttaccgt ataatgggca gaaagtggca gtattttata gaagaggggt
     9781 atctcctgcc tgcggtgttt gttcaggtat tgttattgag cattttactt atatctctcc
     9841 cggcgattag taagctcaag gccaaggttg aggttaagat gaaaaaaaca aataaaccca
     9901 tcctcaacat tgacctgttc tactttgcct tcctcggtct gggattcatg ttcgtggaga
     9961 tcgccctgat tcagaaaatg atactgccgc tggagaaccc tt
//
LOCUS       DATKOU010000258         6126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1150985, whole genome shotgun sequence.
ACCESSION   DATKOU010000258 DATKOU010000000
VERSION     DATKOU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150985"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1212)
                     /gene="pheA"
                     /locus_tag="VMU21_12345"
     CDS             complement(136..1212)
                     /gene="pheA"
                     /locus_tag="VMU21_12345"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HUO78354.1"
                     /translation="MSELKNLRGEIDAIDEEILNLLNRRARIVIEVAGIKRKENTRFY
                     SPEREKEILQRLISINKGPFPNSAIKPLFREILSASLSLEQPLKVAYFGPVATFTHLA
                     AMRYFGSSSVLIPAEGIKDVFESVYSGQTEYGVVPVENSNEGVVSYTLDMFMDYDLKI
                     SGEVLLKISHNLLSRSGDKTKIKKIYSHPQPTAQCRKWLERNVPGIPILESTSTARAA
                     EMASREEDAAAIASEVAAKIYDLHFVERHIEDYKDNFTRFFVISLEPSKRTGRDKTSI
                     MFSTKDRPGALFTILEPFKQAKINLTKIESRPSKRKAWEYIFFVDMGGHMDDKKVKKA
                     IEEVKEGCLYLKILGSYPAGGTND"
     gene            complement(1413..1718)
                     /locus_tag="VMU21_12350"
     CDS             complement(1413..1718)
                     /locus_tag="VMU21_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78355.1"
                     /translation="MGLRNLSEAIILQSASDLLGTPRDKDALGFFGGEGFRLCAEMAK
                     MDHDDKIKLLTMLTECISECKNHKSHPDKKLDLPDKKSSKSLTLPRRRRKSAVMNVA"
     gene            complement(1877..4819)
                     /locus_tag="VMU21_12355"
     CDS             complement(1877..4819)
                     /locus_tag="VMU21_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HUO78356.1"
                     /translation="MEDRIRKTGAKIYSSMEGEVPFLFNKKKWMGRLMEWAMKDETFR
                     FQLFRYIDVLPSLRSDALIVKVLKEYFTEAAHTPMILRKGIERISRSSFVPYIAGKVI
                     RAGVESIARQFIAGRGPSDALKPFNTLWRAGFAFSVDLLGEMVVSDREALEYTGNYLS
                     LLDFLHPKVTGWETDPLLERDDKGPIPRIDISLKVSSLYSQMDPIDFEVSIERAKENL
                     RQIFRKAREVGASVCLDMEHYYFKDLILEVFKSILDEFEDYPFAGIALQAYLKDTKED
                     LLSLIEWTKARGRKISVRLVKGAYWDYETAVNRQKGWPVPVFLNKGETDFNYEELTRI
                     LFENSEFIRPAIATHNLRSVSNAIALAESLGLPKDFFEFQMLYGMGEPLRKALRDMGY
                     RVRVYTPVGELIPGMAYLVRRLLENTSNESFVRQFFAEGKPLEELIKAPKIETAVIAE
                     RREEGIFRNQPPMDFSQAENRERMKDSLKKMKNDFNRRYPLSIGNQEIFTDREIVSNN
                     PANPREVIGRVSLATKKEAERAIEEAKRAWDKWRKRVPRERAEYLFRVAEEMRKMRSE
                     LIALEIYEVGKTWKDADGDVSEAIDYCEYYGREMIRLGTPMRLGDYPGEENEYHYEPK
                     GVGVVISPWNFPLAIPAGMISAGIVAGNCIIFKPSGLSPVTGWKLSDIFRFAGIPPGV
                     LQFLPGPGNEVGEFLVSHPGIDFVAFTGSKDVGLDIVKRAGETRSGQRNIKKIIAEIG
                     GKNAIIIDETADMDEALTGVLESFLGYQGQKCSACSRVIVIGEVFDEFCGRLRGAAES
                     IRIGPPEEPGTFMGPVISEEALKRIGRYRERGMNEGKPLLLRRAEGEGYFIGPAIFGD
                     VDHGSPIAQEEIFGPVLVVMRARDMDEAIDIANDSSYALTGGIFSRSPANIRKAEEGF
                     RAGNLYINRKITGALVGRQPFGGSGMSGVGSKAGGPDYLLQFMNPRSISENTLRRGFA
                     YSVRSPKK"
     gene            complement(4908..>6126)
                     /locus_tag="VMU21_12360"
     CDS             complement(4908..>6126)
                     /locus_tag="VMU21_12360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HUO78357.1"
                     /translation="KTLIYSADRVTEIKESAKLGIHAEIARLDFPVIMQRMKGAVESG
                     RNSIMEAIKDSEHLDFYHGEGHFVRDYTLEVNGEKIKGKKIFIAAGARPIIPPIKGLE
                     GVEFLNNENILELGKNPESLLIIGGGYVAVEYGHFFAAMGTKVTMVQRNERLLPNEEP
                     EITDLLIKEMGKRMKIQTNAEVIELTQCVDGYTVVTADRNAGARRPITVERIMVATGR
                     RSNAGLLRVEHTGVETDEANFIRVNDHLETNKRNIWALGDIIGKQMFTHAGDREAEVA
                     WHNATHKKKMKMDFSAVPHAVFTHPQIASIGLTEEQAKKEHEILVGKAKYADTVMGTA
                     MVEEEGFAKAVVEKGTKKILGFHIIGPEASLLIQEVVNAVANKNDISYITDSMHIFPA
                     LSELIPQTLNNLE"
BASE COUNT         1391 a         1672 c         1314 g         1749 t
ORIGIN      
        1 gccttgggcg atggtccgag agggttctcg ttggatgcga gtttgattga acccgagatg
       61 ccaagctccc tctccagctc ttccaccggt ttcccgggaa cataaggcct tacggcagag
      121 acatactccg ggggcctaat cattggtccc ccctgcagga taagaaccga ggatcttcag
      181 gtagaggcag ccctctttca cctcctctat ggcctttttg actttcttgt catccatatg
      241 tccccccata tccacaaaaa atatgtattc ccacgccttc ctcttggatg gtcgcgattc
      301 gatctttgtg agatttatct tcgcctgttt gaaaggttca aggatggtga agagagcacc
      361 gggcctgtcc ttggtcgaga acatgatgga cgttttatcc ctgcccgtcc tctttgaagg
      421 ctccaagcta atcacgaaga accgtgtaaa gttgtcctta taatcctcta tatggcgctc
      481 tacaaagtgc aggtcataga tctttgccgc gacttcgctt gcaatggcgg cagcatcctc
      541 ctcccttgag gccatctctg ccgcccttgc agtgctggtc gattcgagga tcggtattcc
      601 gggcacattc ctctccagcc acttgcggca ttgggcggtg ggctggggat gggaataaat
      661 tttctttatc ttcgtcttat ccccgctcct ggacagaagg ttatgggata tcttcagcag
      721 cacctcaccc gagatcttca ggtcataatc cataaacata tcaagggtat agctcaccac
      781 gccctcgttg gaattctcta cgggcacgac gccgtattcc gtctgtcctg agtatacgga
      841 ttcaaacacg tccttgatgc cttcggcggg aataaggacc gaggacgagc cgaaatacct
      901 catggcagca aggtgtgtga atgtggcaac agggccgaaa taggctacct tcaaaggctg
      961 ttcaagggaa agtgaggcgg agaggatttc tctaaaaagc ggtttgatag cactgttggg
     1021 aaacggaccc ttgttgatgg agataagcct ctgcaagatc tccttttcac gctccgggga
     1081 ataaaagcga gtattctctt ttctcttgat gcctgcaacc tcgatgacga ttctggccct
     1141 cctgttgaga aggttcagta tctcttcgtc tatcgcgtcg atctcgcccc tcagattttt
     1201 cagctcactc atcgtctttt cccctttgtt tttgtgatta gaatattaat aatacagatt
     1261 ttttcttaat tttttcaagg acttcagata gtgaaaggag gaagttccaa aacaatgtga
     1321 tgcactgttc gccggaaacg gcgattaaaa ttcaggatca aacagagaac caataaaaag
     1381 aaggcccgca cacagagggg gaaggatatt cttcaggcta cattcatcac tgcgctcttt
     1441 ctgcgccggc gaggaagggt caagcttttc gaggatttct tatccggcag atccagtttc
     1501 ttatccggat gactcttgtg atttttgcat tcggaaatgc attccgtgag catggtgagg
     1561 agcttgatct tatcgtcgtg atccatcttt gccatctctg cacagagcct gaagccttct
     1621 cctccgaaga atccaagagc atctttgtca cgcggggtac ccaaaaggtc tgatgcggat
     1681 tgaaggatta ttgcctctga aagatttctc agacccatga catcctcctc ccacacaggt
     1741 gattatgata actataccca agagagatga taaagatata tcagcgtaaa taggattttt
     1801 ttgtaagaat tcaggaatag ggatttgtag ggtttttccc tacaaggaat gctttgttcg
     1861 ttaaactcct tcagccttac tttttggggg acctgacaga atacgcaaag ccccttctca
     1921 gggtattttc gctaatactc cttggattca taaactgaag aaggtagtcc ggcccccctg
     1981 ccttggaacc gacccctgac attcccgacc ctccaaaggg ctgcctcccc acaagggcac
     2041 cggtgatttt cctgtttatg tacagattcc ctgccctgaa tccctcttcc gccttcctga
     2101 tattggcagg gctcctcgaa aatatacctc ctgtcagtgc ataagaggaa tcattggcga
     2161 tatcaatagc ctcatccatg tcccttgccc tcataacaac aagcactgga ccgaatatct
     2221 cttcctgcgc aatgggagag ccgtggtcca catccccgaa tattgcaggc cctataaaat
     2281 aaccttctcc ctctgccctt ctgaggagga ggggcttccc ctcgttcatc cctctctctc
     2341 tgtatctgcc aattcttttc aatgcctctt cggaaatcac cggccccata aaggtgcctg
     2401 gctcttccgg agggcctatc cttatgctct ccgccgcccc cctgagcctg ccgcagaatt
     2461 catcaaagac ctcacctatt acgatcaccc ttgagcaggc cgaacacttc tgtccctgat
     2521 atccaaggaa agattctaaa acccctgtta acgcctcatc catatcggca gtttcatcaa
     2581 taatgatagc gttttttcca cctatctctg caatgatctt cttgatattc ctttgaccgg
     2641 agcgggtctc tcctgcacgc ttcactatat ccagacccac atcctttgag ccggtaaaag
     2701 cgacgaagtc tattccaggg tgagatacga gaaattctcc cacctcattt cccggacctg
     2761 gaaggaattg caggaccccg gggggaattc ctgcgaatct gaagatgtcc gagagcttcc
     2821 atcctgtaac aggggaaagg ccagaaggtt tgaaaatgat acagttccct gcaacgattc
     2881 ctgctgatat cattcctgcg ggtatggcaa gggggaaatt ccagggagat atcacgaccc
     2941 ctactccctt tggttcgtaa tgatattcgt tttcctcacc cggatagtca ccaagccgca
     3001 ttggggttcc gagccgtatc atctccctcc catagtattc gcaataatct atggcctcgc
     3061 tcacatcacc gtctgcatcc ttccatgtct tgccgacctc atatatctca agggctataa
     3121 gctcagaccg catttttctc atctcctctg caactctaaa aaggtattca gccctctccc
     3181 tgggaaccct cttcctccac ttatcccatg cccttttcgc ctcttcaatg gccctttcag
     3241 cctccttctt tgtggcaagt gatacgcggc ctattacctc ccggggattt gccgggttgt
     3301 tagatacgat ttcacggtcc gtaaatatct cctgattccc tatggaaagg gggtatcttc
     3361 tgttaaaatc atttttcatc ttttttagac tgtccttcat tctttccctg ttttcggcct
     3421 gggaaaaatc catggggggc tggtttctga atatcccttc ttcacgacgt tcggcaataa
     3481 ccgctgtttc aatcttcgga gcctttatca gttcctccaa tggttttccc tctgcaaaga
     3541 actgcctcac aaaagactca ttggaggtat tttctaaaag ccttctcacc agatatgcca
     3601 tccctgggat gagttctccc acaggggtat acaccctcac tctatatccc atatcccgca
     3661 aggcttttct caggggctca cccatgccat agagcatctg aaactcgaag aagtccttgg
     3721 ggagtcccag agattcagct aacgcaattg cattgctcac gctcctcaga ttatgtgtcg
     3781 cgatggcagg gcgtatgaac tcagaatttt caaacagtat ccttgtgagc tcttcgtagt
     3841 taaagtctgt ctcgcccttg ttcaggaaca caggcacagg ccatcccttc tggcggttta
     3901 ctgctgtctc ataatcccag tatgcccctt ttacaaggcg aacggatatt tttcttcccc
     3961 tcgccttcgt ccactcaata aggctcagga ggtcttcctt tgtgtccttc agatatgcct
     4021 ggagggctat ccctgcaaag ggatagtctt caaactcatc aagaatgctt ttgaagacct
     4081 caaggataag gtccttgaag taatagtgct ccatatcgag acagacggac gctccgacct
     4141 cccgggcctt tctgaatatc tgcctcaagt tctccttggc cctctcgatg gagacttcaa
     4201 agtctatagg atccatctgg gagtaaagag aagatacctt gagcgagata tcaatccttg
     4261 gaatgggtcc cttgtcatcc ctttcaagga gggggtctgt ttcccagccc gtgaccttgg
     4321 ggtgaagaaa gtccaggagc gagaggtagt tcccggtata ctccagtgcc tcgcggtcac
     4381 tcaccaccat ctcacccagc aggtcgacac tgaatgcaaa accagccctc cagagcgtgt
     4441 taaacggttt cagtgcgtcc gagggacccc ttcccgcgat gaactgtcgt gctatggatt
     4501 ccacgcctgc cctgatcacc ttaccggcga tgtaagggac aaagctgctc ctcgagatgc
     4561 gttctatacc cttccttagt atcatgggag tatgtgccgc ttccgtaaaa tattctttca
     4621 gtacctttac gataagggcg tcactccgga gggaaggcag gacatcgata tacctgaaga
     4681 gctggaacct gaatgtctca tctttcatag cccattccat tagccttccc atccacttct
     4741 tcttattaaa gaggaaggga acttctcctt ccatggatga ataaatcttt gcccctgtct
     4801 ttcttatacg atcttccaaa tcattatgcg ggttcacaca gagacaccaa agacctttat
     4861 ttcctttgag agaaagatag cctgaacagt tctcactccc atgagtgcta ttcgagatta
     4921 ttaagcgtct gcgggataag ctctgagagt gcggggaata tatgcatgct gtccgtgata
     4981 tatgagatat cattcttgtt tgctactgca ttcaccactt cctggatgag taaggaagct
     5041 tcgggtccta tgatatggaa gccaagaatt ttctttgtcc ctttttcgac cactgccttt
     5101 gcaaacccct cttcctccac cattgcagta cccattaccg tatccgcata ttttgccttt
     5161 cctacgagaa tctcatgctc tttcttcgcc tgctcttcgg taagcccgat agatgcaatc
     5221 tggggatgtg taaatactgc gtgggggaca gcgctgaaat ccatcttcat tttcttctta
     5281 tgggtggcat tgtgccacgc cacttctgcc tctctgtcgc ctgcatgagt gaacatctgt
     5341 ttgccgataa tgtcacccag tgcccatatg ttccttttgt ttgtttcgag atgatcgttc
     5401 actcttatga aattggcctc atcggtctct actcccgtat gttcgaccct taacaaacca
     5461 gcgtttgacc tcctccctgt tgcgaccatg atacgttcta cggtaatagg ccttcgtgca
     5521 cctgcatttc tgtccgcagt aaccacggta tagccatcga cacactgtgt aagttctatt
     5581 acctctgcgt ttgtctgaat tttcatacgt ttccccattt cctttatcag caggtctgta
     5641 atctccggct cttcattggg aagcaacctc tcgttcctct gcaccatcgt cacttttgta
     5701 cccatagccg caaaaaagtg gccgtactcc acggccacgt aaccgccgcc gatgatgagt
     5761 aggctctcgg ggtttttccc aagttcaagg atgttctcat tgttcaagaa ctccacgccc
     5821 tccagtcctt taatgggtgg aatgattggt ctggctcctg cagcgataaa tatcttcttt
     5881 ccctttatct tctcgccatt cacctcaagg gtgtagtccc tcacaaaatg tccttctccg
     5941 tgatagaaat cgagatgctc cgaatctttt attgcctcca taatggagtt ccggcctgat
     6001 tccactgcgc ctttcatccg ctgcattatg acgggaaaat ccaaacgggc tatttccgca
     6061 tgaatcccca gtttcgctga ttcctttatt tctgttaccc tgtcagcaga atatatcagt
     6121 gtcttt
//
LOCUS       DATKOU010000259         8135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3070938, whole genome shotgun sequence.
ACCESSION   DATKOU010000259 DATKOU010000000
VERSION     DATKOU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070938"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VMU21_12365"
     CDS             complement(<1..553)
                     /locus_tag="VMU21_12365"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HUO78358.1"
                     /translation="MRIDREFAFRLLEGARKRGADLAEVYLKSSRSLSVEVRDQRTDA
                     LESSTDFGYSLRVIRDKRLGFSFATAQGDADSVIARAVEASRWTVQDEWLDLPGPSEQ
                     TSLDIFDEAIASMDEGEAIEKALKIEKAALDADARIRKVRKASASFSQRDRLIMNSKG
                     LEKAYSATACVAQIMVVAENGSDS"
     gene            complement(543..914)
                     /gene="queD"
                     /locus_tag="VMU21_12370"
     CDS             complement(543..914)
                     /gene="queD"
                     /locus_tag="VMU21_12370"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HUO78359.1"
                     /translation="MFELMIETTFSAAHQLRGYRGKCEYLHGHNWKVQVHVSAEKLNE
                     IDIAMDFHELKRLTHEVTAPLDHTFLNDVFPFTEKNPSSENIAKWIYDSLKKKITDDH
                     IQVTAVSVWESDTASATYYED"
     gene            complement(980..1501)
                     /gene="hpt"
                     /locus_tag="VMU21_12375"
     CDS             complement(980..1501)
                     /gene="hpt"
                     /locus_tag="VMU21_12375"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HUO78360.1"
                     /translation="MVVGKPLFTVEQIQQRVRELAGRISEEYEGKNLLAVGILRGAFM
                     FMSDLVRAIRVPLTVDFIIASSYLKTDTTGDVKVHYDIREDVTDKDVLFVEDIVDTGI
                     TLNQLRERILARHPRSLRICSFLDKRERRKVDVPLDYVGFEIPNQFVVGYGLDYENKF
                     RNLPYIAVFKKSV"
     gene            complement(1557..3047)
                     /locus_tag="VMU21_12380"
     CDS             complement(1557..3047)
                     /locus_tag="VMU21_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HUO78361.1"
                     /translation="MAHCKCGASLTKRKFLDSCKNGYGPVSCLSAEIPYTSPERLYPL
                     FSGSGSFVFESVKGPEKIARYSFIGFDPYLSMRAKEKKIAVRSGTSQSISYAHPLKLL
                     GELLEGYAQKPVDGLPPFQGGAAGLLSYDFSRYLERLPDTTIDDLQIPDAHFFFIDRL
                     ISFDHKTRKAWAIVCPGVRRKACVDAGLWYEEAESVLDEICRRIESTAWEDASHTIHP
                     VRRGIEIIHGMKKKEYMDMVIRAKEYIAAGDIFQANLSHRISAHLGEASPWELYRILS
                     AMNPSPFAGFADFGEYWIVSSSPERLVKVKDGTVETRPIAGTRPRGKDMREDELMRAE
                     LLLNEKERAEHIMLIDLERNDLGRVSMYGSVEVDELMITEDYSHVMHIVSNVRGKLMH
                     RKTCLDVIRAVFPGGTITGVPKVRCMEIIDELEPVRRGPYTGSLGYLGFSGVMDMNII
                     IRSFVVKNGHAYVQAGAGIVADSDPEREYHETIKKAEALIASLGRC"
     gene            complement(3058..3777)
                     /gene="pyrF"
                     /locus_tag="VMU21_12385"
     CDS             complement(3058..3777)
                     /gene="pyrF"
                     /locus_tag="VMU21_12385"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HUO78362.1"
                     /translation="MPVKEKIILALDVSDPAYAIEIVDRFKDYVGVFKVGLELFISGG
                     PGVVKEINKRGKKVFLDLKFHDISHTVIKAALAATRLRVYMFNLHASGGLDMMRTCRE
                     AVVDLCLKENIQRPRMLGVTVLTGLTQEVLKGELGIQHSLRTHVKELSRLSMHAGLDG
                     VVASGHEVAMIRNHFGKDFLIVTPGIRTSWSPPDDQRRTMTPREAIREGADYIVLGRA
                     VLNQADPFKALELITTEILTA"
     gene            complement(3779..4327)
                     /gene="dcd"
                     /locus_tag="VMU21_12390"
     CDS             complement(3779..4327)
                     /gene="dcd"
                     /locus_tag="VMU21_12390"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HUO78363.1"
                     /translation="MVRNDRWIRDMAPKGMIQPFHESQVSEGVISSGVSSYGYDMRIS
                     DEFKIFTNINTTVVDPKNFDPRSFVDFKGDICIVPPNSFALGRSVEYFRIPRDTLVIC
                     LGKSTYARCGIVVNVTPLEPEWEGHVTIEISNTTPLPAKIYANEGIAQLIFLVASEVC
                     GVSYADKSGKYQAQRGITLPRM"
     gene            4443..5030
                     /locus_tag="VMU21_12395"
     CDS             4443..5030
                     /locus_tag="VMU21_12395"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HUO78364.1"
                     /translation="MAELELPNAEELEEVRSKGFTRRVALITAIFAVMLAISSLGGNN
                     AMKEMILAQQQSSDQWSFYQAKVVREHLYKSQKLRIEADLLERGAMMKPNVRAHYEST
                     LKKLNEEEARYREEKKEIEQEAKKLEHERDMNRSKDPYFDYAEVLLQIAIVMASISIL
                     ALSRPVFYFAVVVAAIGALMSLNGFFLLFRIPFLH"
     gene            complement(5134..5322)
                     /locus_tag="VMU21_12400"
     CDS             complement(5134..5322)
                     /locus_tag="VMU21_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78365.1"
                     /translation="MIMAQCPACTTHRSHTETFDKILSALVNELKERKLSGVDDVDEL
                     AMALSVLVADALKIDIYQ"
     gene            complement(5420..5848)
                     /locus_tag="VMU21_12405"
     CDS             complement(5420..5848)
                     /locus_tag="VMU21_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HUO78366.1"
                     /translation="MKTKRIIILCIIVACSMLCVSMVIADQKGEELAAKAASKWLALV
                     DEGKYSESWDEAAQLFKGAVTKEQWKESVSSARKPLGKLVSRTLKSKTYATSLPGAPD
                     GEYVVIQYTTSFENKKAAVETVTPMLDKDGKWKVSGYYIK"
     gene            6079..6906
                     /gene="lsrF"
                     /locus_tag="VMU21_12410"
     CDS             6079..6906
                     /gene="lsrF"
                     /locus_tag="VMU21_12410"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HUO78367.1"
                     /translation="MDFGMKNRLSKIFKPKTGKTVMLAVDHGYFQGPTTGLRDMKGIT
                     PLISHADCLFVTRGILRTSIDPGTDTSICLRVSGGPSILGELSREDITTSMEEALRLN
                     VSGVGMSIFVGAEDEERTIANLGRLINEAERCGMPVLAITAVGKEMVRDSRYIGLACR
                     IAAEIGAHIVKTYYCDDFHKVVEGCPVPIVIAGGKKIPEMDALLMTEGAIKAGASGVD
                     MGRNIFQSDNSVGMIKAVRAIVHKGASAKEAFEIYQKTKRPPGAGGEQIPDESLEKV"
     gene            6954..7982
                     /locus_tag="VMU21_12415"
     CDS             6954..7982
                     /locus_tag="VMU21_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUO78368.1"
                     /translation="MKVAVYYSNDDVRIEERPTPAISDGELLVRMMASGICGTDVMQW
                     YRQKKGPRVLGHEMAGEIVDAGSRVEKFRIGDRVFVSHHVPCYRCNYCSEGNYTACES
                     LHTGNYDPGGFSEYIRVPEQNVKFGTFLLPNGMTYEDAAMIEPLACVVAGQRELGLRE
                     RHTLLVIGCGISGLLHIQMAKAAGSMVVATDINGFRLDKARELGADYVFPADDFSADK
                     LRVINKERLAERVVVCAGVMQAVESAIASVDRKGKILFFAVPKEDIPLPSLRFWRDEI
                     TVAFSYGAAPGDLQKAMELISSGTVNVRKMITHGVPLSEIQTGFRLVSEARESLKVVV
                     VPDKSFYT"
BASE COUNT         2002 a         2155 c         1889 g         2089 t
ORIGIN      
        1 gactgtcact gccattctct gcaacaacca ttatttgagc aacgcaggca gtagcagaat
       61 acgccttttc gagccctttt gaattcatga ttaacctatc tctctgggaa aaggacgcag
      121 aggcctttct cacctttctg atccgtgcat cagcatcaag agcggccttc tctatcttaa
      181 gtgctttctc gatggcctcc ccttcatcca tggacgcaat ggcctcatca aagatgtcaa
      241 gagatgtctg ctccgaaggc ccgggaaggt cgagccactc gtcctgcaca gtccatcgtg
      301 atgcctcaac agcgcgcgct ataaccgaat ccgcatctcc ctgtgccgtg gcaaacgaaa
      361 agccaagtct cttgtcccta atgacccgga gggaatatcc gaaatccgtg gaggattcca
      421 gggcatctgt cctctggtct ctcacctcca cggaaagact gcgtgaagac ttaaggtaca
      481 cctcggccag gtctgccccc cttttccttg ctccttcgag aagccggaag gcgaactcgc
      541 ggtcaatcct catagtatgt tgcagaggca gtatcagact cccatacgct caccgcggtt
      601 acctggatgt ggtcatcggt gatctttttc ttaagggaat catagatcca tttagcaata
      661 ttttccgagg acggattctt ctcagtgaag ggaaacacgt cgttcaggaa tgtgtggtca
      721 aggggcgcgg tcacctcatg agtgagacgc ttcagttcat ggaagtccat ggcaatatcg
      781 atctcgttga gcttctccgc agagacatgc acctgcactt tccagttgtg accatgcagg
      841 tattcacact ttcccctata ccccctcagc tgatgtgctg ctgaaaacgt cgtctcgatc
      901 ataagttcga acatatcaac cctttctcat atagactttc ttccagagac gcgataccct
      961 ctattctgcg tcagcagtgt cagaccgact ttttgaagac cgcgatgtac ggaaggttcc
     1021 tgaatttatt ttcgtaatcc aggccatacc cgaccacaaa ttgattgggt atctcaaacc
     1081 ccacatagtc cagggggaca tccaccttcc ttcgctctct tttatccaga aatgagcata
     1141 ttctaagact tctcgggtgc cttgcgagta tccgctccct cagctgattc agggttattc
     1201 ccgtatcaac gatatcctcc acaaacagga catccttgtc cgtcacatcc tcccttatgt
     1261 cgtaatgtac cttcacgtca cctgtggtat ctgtcttcag ataacttgac gcaatgataa
     1321 aatccacagt gagcggaacc ctgatcgctc ttaccaggtc cgacatgaac ataaaggcac
     1381 ctctcaggat tcccactgcg agcaggttct tcccctcata ctcctcagaa atcctccctg
     1441 ccaactctct cactctctgc tgtatctgtt ctacggtaaa gagcggttta cccactacca
     1501 tcaattcttc ctcccttggt atatgatttg attatattcc taaatcaccc ggcaactcaa
     1561 cacctcccaa gtgaagcgat cagtgcttca gccttcttaa tagtctcatg gtactccctc
     1621 tccggatcag aatccgcaac tataccagcc ccagcctgta catacgcatg tccattcttg
     1681 accacaaaag acctgatgat gatattcata tccataaccc cggaaaaccc cagatatccc
     1741 agagaaccgg tatacggacc cctcctcaca ggctccaatt catctatgat ctccatacat
     1801 ctcaccttgg ggacccccgt gatggtacct ccggggaaaa ccgcccgtat gacatccagg
     1861 cacgtcttcc tgtgcataag ctttcccctt acgttggata cgatatgcat aacgtgggag
     1921 tagtcttccg tgatcataag ttcatccact tcaacgcttc cgtacattga aaccctcccc
     1981 aggtcgttcc gctcaagatc gatgagcatg atgtgttctg cccgctcctt ctcgttcagg
     2041 agcaactctg ccctcatcaa ttcatcttct ctcatgtcct ttccccttgg tctcgttccc
     2101 gcaattggtc tagtttccac tgtaccgtcc ttgaccttta ccagtctctc gggagaagaa
     2161 ctcacgatcc agtactctcc aaagtcggca aaaccggcaa aaggggaagg gttcattgcg
     2221 ctgagtatcc ggtaaagttc ccatggactc gcctctccta aatgcgcgga gatcctgtgg
     2281 gaaaggtttg cctggaatat atcacccgca gcaatatatt cctttgccct tatcaccata
     2341 tccatatact ctttcttttt cattccgtga ataatctcaa ttccccgcct gacagggtgg
     2401 atagtatgcg acgcgtcttc ccacgcggta gattctatcc ttctgcatat ttcgtcaagg
     2461 acagactcag cctcctcgta ccataaccct gcatcaacgc acgcctttct ccgcacaccc
     2521 ggacacacaa tagcccacgc ctttctcgtc ttatggtcaa aggagatcag tctgtctata
     2581 aagaaaaaat gggcatcagg aatctggagg tcatcaatag tggtgtcggg aagcctttcc
     2641 agatagcggg aaaaatcgta actaaggagc ccggctgccc cgccctggaa agggggaagt
     2701 ccatcaacag gtttttgcgc gtagccttcc aaaagttcac ccagcagctt caggggatgt
     2761 gcgtaggata ttgactgtga cgttcctgat cgtactgcaa tttttttctc ttttgcacgc
     2821 atgctgagat acggatcaaa tccgataaag gagtaccggg cgatcttttc gggtcctttc
     2881 acactttcga agacaaagga gccgctccct gagaaaaggg gataaagtct ttcgggtgaa
     2941 gtgtagggga tctccgcaga gagacaggac actggtccgt agccattctt gcaggaatcc
     3001 aggaactttc tcttggtgag ggatgcaccg catttacaat gtgccacgtg tgccctctca
     3061 ggcagtaagg atttcggtgg taatcaattc aagggcctta aaaggatccg cctgattgag
     3121 cacagccctt ccaaggacga tatagtcagc cccttccctt atcgcctccc ggggtgtcat
     3181 ggtgcggcgc tggtcatcag gagggctcca cgaggttctt atgccagggg tcacgatgag
     3241 aaaatccttc ccgaaatggt ttctgatcat tgccacttca tgccctgatg ccaccacgcc
     3301 gtcaagaccc gcatgcatgg aaagtctgga gagttccttc acatgggtcc tcaaactgtg
     3361 ctggattccc agttcaccct tcaggacctc ctgggtcaat ccggtcagca cagtcacccc
     3421 cagcatcctc ggcctctgga tgttctcctt cagacagaga tccaccaccg cttccctgca
     3481 tgtgcgcatc atgtccagcc cgcctgaggc atggagattg aacatgtata ctctcagccg
     3541 ggtcgcggcc aatgcggcct tgatgacggt gtgggaaatg tcatggaact tgaggtcgag
     3601 aaagaccttc tttccccttt tgttgatttc cttcaccact ccaggccctc ctgatatgaa
     3661 tagctcgaga cccaccttga acacgcccac ataatccttg aagcgatcca cgatctctat
     3721 tgcatacgcc ggatctgaaa catcgagggc gagaataatc ttttccttca ccggcatgct
     3781 acatccttgg gagggtaatg cccctctgtg cctgatactt tcctgatttg tctgcatatg
     3841 aaaccccgca cacctctgac gcgacaagga atataagttg cgcaatgcct tcgttcgcgt
     3901 agatcttcgc aggcagaggc gtggtatttg atatctcgat cgtcacatga ccctcccatt
     3961 caggttcaag gggggtgacg ttcaccacaa ttccgcatct cgcataggtt gattttccca
     4021 ggcatattac gagcgtatcc ctcggaattc tgaaatactc cacagagcgg ccaagggcaa
     4081 aggaattggg agggacaatg catatatccc ccttgaaatc cacaaagctc ctgggatcaa
     4141 agttttttgg gtcaactacc gtggtattga tgtttgtaaa tatcttaaat tcatcggaga
     4201 tcctcatatc gtacccgtaa gaactcacgc ccgaggatat cacgccctca ctcacctggc
     4261 tttcatgaaa aggctggatc atgccctttg gggccatatc ccttatccac cggtcattcc
     4321 tcaccatatg agccctccat ttttggaaaa gattaccata aaaacttcct ggatgacaag
     4381 gaatatcatc tgtagtagaa taatgaaaat tcacatgagg gaaaaggagg caatgcagaa
     4441 ccatggccga gttagaactc ccaaatgccg aagagcttga ggaagtgaga tcaaagggtt
     4501 ttacaaggcg agttgccctt atcactgcaa tattcgcagt catgcttgcc atctcctccc
     4561 ttggcgggaa taatgcgatg aaggagatga tcctcgccca gcagcagtca tccgaccagt
     4621 ggtcctttta ccaggcaaag gtggttcgtg aacatctcta caaaagccag aaacttcgca
     4681 ttgaagcaga cctccttgaa cggggagcaa tgatgaaacc caatgtgcgt gcacactatg
     4741 aatccacact caaaaagctt aacgaggaag aggctcgcta tcgcgaggaa aagaaagaga
     4801 tagagcagga agcaaagaaa ctggaacatg agcgtgacat gaatcggagc aaggaccctt
     4861 acttcgacta tgcggaagtc ctcctccaga tagcaattgt catggcatcg atctcgattc
     4921 tcgccctttc acgcccggtc ttttatttcg ccgttgttgt tgctgcaatt ggggctctaa
     4981 tgtctcttaa cggtttcttt ctgcttttca gaatcccttt tttgcactag accaaggcta
     5041 tgcgaggcat tcgatccaga gcaatcatat tctccctttt cccttgttgc tctctttctc
     5101 ttttggctcg tcagatgcat ggctttacct cagctactga taaatatcaa tcttcagggc
     5161 atctgcaacc aggacggaaa gtgccatagc cagctcatcc acatcgtcaa cgcccgaaag
     5221 tttccgttcc tttagttcat tgacaagagc agacaaaatc ttgtcaaatg tttctgtatg
     5281 actgcggtgc gtcgtacacg caggacactg agccataatc atctccgcct tttgaatacc
     5341 ctctattttc tatttttcaa aatctgaact atcatccgag cgtattcatc taatctgctc
     5401 tctgccaaac attccctgat tacttaatgt agtaccccga caccttccac ttaccgtcct
     5461 tgtccagcat gggagtgacg gtctcgaccg cagctttttt gttctcaaag gaggtagtat
     5521 actgaatcac gacatactct ccatcagggg cgccaggaag ggatgtggcg tatgtctttg
     5581 acttgagtgt ccttgagaca agcttgccaa ggggctttct cgcgctgctg acggactctt
     5641 tccactgctc tttggtgacc gctcccttga acaattgcgc agcctcgtcc cagctctcgg
     5701 aatattttcc ctcgtctact aatgccagcc attttgaagc agcttttgca gcaagctctt
     5761 ctcctttttg atcagctatt accatagaaa cgcacagcat actgcacgcg acgatgatgc
     5821 acaggataat tattctcttt gtcttcatac tcctcctctt ttcttgcaat agatacccta
     5881 gtatactttt ttcgtctcct gatatccaat atcttccgct tgttagataa ggggcctagt
     5941 tttatcgact cattggcgaa atttaatgat atcgattaca atagtgaacc gttttcatga
     6001 tgcaacaacg tccgaaatat aaaccagagg ccaaatgctc aaagggctta cccgacggcg
     6061 atataaggag gagataatat ggattttgga atgaaaaaca ggctgtcaaa gatttttaaa
     6121 cccaaaaccg gaaagaccgt tatgcttgcg gttgaccacg gctattttca gggccccacc
     6181 acaggattga gggacatgaa aggcatcacc cctcttattt cccatgcaga ctgtcttttt
     6241 gtcacaaggg gaatattaag gacttccatt gatccaggaa ccgatacgtc catctgtctt
     6301 agggtctccg gcgggccgag catcctgggt gagctgtcac gcgaggatat caccacatcc
     6361 atggaagagg ccctcaggct caatgtctct ggagtgggaa tgtccatttt tgtaggagca
     6421 gaagacgagg aaaggaccat tgcaaatctc gggaggctga tcaatgaagc ggaaaggtgc
     6481 gggatgcctg tgcttgccat caccgctgtg ggcaaggaga tggtgagaga ctcgcgctat
     6541 atcggcctcg cgtgcaggat tgccgcagag ataggcgcgc acattgtcaa gacatactac
     6601 tgcgatgatt tccataaagt agttgaggga tgccctgtgc ccattgtcat tgcaggggga
     6661 aagaagatac ccgagatgga cgccctttta atgacagaag gcgcgatcaa ggccggcgct
     6721 tcaggtgttg acatggggag gaatattttc cagagcgaca actcggtggg tatgataaaa
     6781 gcggtgaggg ccattgttca caagggcgcc tcggcaaaag aggcatttga gatttatcag
     6841 aagacaaaga gacccccggg agcaggagga gagcaaatcc cggacgaaag cctggaaaag
     6901 gtataaggta taatgaagat tcacaaatct ttaacctcag tctgtcttgt tctatgaaag
     6961 tggcggtgta ttactctaac gatgacgtga ggatagagga gcgccctacc cctgccatat
     7021 cagatggtga acttttagtg aggatgatgg cgtcgggcat ctgcggcact gatgtgatgc
     7081 agtggtacag gcaaaaaaag ggtccccgtg tgttaggcca cgagatggca ggagagatcg
     7141 ttgatgcggg gagcagggtt gaaaagttta gaattggcga cagggtcttt gtgtcccacc
     7201 atgtgccctg ctaccgctgc aactactgca gcgaaggcaa ctacactgcg tgcgaatcgc
     7261 tccataccgg aaattatgat ccaggcggct tttctgaata cataagggtt cctgagcaga
     7321 atgtgaagtt cgggacattc cttctcccga atggaatgac ctacgaagac gctgcaatga
     7381 tcgaacccct tgcatgcgtg gtggcgggtc aaagagagtt agggctcagg gaaaggcata
     7441 ccctccttgt cataggctgc ggcatttcag ggctcctgca catccagatg gccaaagcgg
     7501 cgggatctat ggtcgtggca acagatatta atggcttcag gctggataag gccagggaat
     7561 tgggcgcaga ttacgtgttc cctgctgatg acttttctgc ggacaagctg agggttatca
     7621 ataaggaacg acttgcagag cgcgtggtcg tatgcgcggg agtaatgcag gcagtagaga
     7681 gcgccatcgc ttccgttgac cgcaagggga aaatactttt ttttgcagtg ccaaaggagg
     7741 acatcccttt gccatccctc agattctgga gagatgagat caccgtggca ttctcctacg
     7801 gtgccgcacc cggagacctc cagaaagcaa tggagctcat tagcagcggg acggtcaatg
     7861 taagaaagat gataacccat ggggtccctc tctcggaaat tcagactggc ttcagactgg
     7921 tctctgaggc gcgggagtct ctgaaagtag ttgtcgtgcc tgacaagtcc ttttacacat
     7981 aaatccaccc ccgtatcttg acccctcagg cgtttttggt ataccgtatt ccatgttttc
     8041 tgcaaatcgt tttctgagaa tcttttctct cctcatcggc ctggcaggtc tttgaccagg
     8101 gtgatattgt cgagatagac tcccttgaaa acatt
//
LOCUS       DATKOU010000260         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_1153791, whole genome shotgun sequence.
ACCESSION   DATKOU010000260 DATKOU010000000
VERSION     DATKOU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153791"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VMU21_12420"
     CDS             <1..938
                     /locus_tag="VMU21_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HUO78369.1"
                     /translation="HLLQASLRSVLGDHVKQAGSFVSPERFRFDFTHFSGLEESEVAS
                     LETMVNETILENIPVNTSVMDIKSAIDSGAMALFGERYGDSVRVVSVPGVSSELCGGT
                     HCKATGDIGLFAIVSEGSVSSGIRRIEGFTGGTAFQYLKEKALELKKIDALLKTDHAY
                     PKIERLLSELKALEREREEVKAKAAVKDASAIIEGARRVNGIVVISHRVDDLEQKDLR
                     TLADTLRDKIGSGIIFITSVRDGQASMLAVVTKDLVGRFNAGEILKEVATMTGGRGGG
                     KAEMAQGGTNKLDTLDSAMESVYGIIRERSSAAIG"
     gene            1006..1332
                     /locus_tag="VMU21_12425"
     CDS             1006..1332
                     /locus_tag="VMU21_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78370.1"
                     /translation="MTFFDVVRNALLVGLGVQEKVNEFVNELVKKGELSESQGAKLLR
                     EWTEKADKGTEQFSKGLSDMISKTMEKMTLPSKDDIEKLNRKIQALSVRVKKLEGVKG
                     AETEEE"
     gene            1338..3035
                     /locus_tag="VMU21_12430"
     CDS             1338..3035
                     /locus_tag="VMU21_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HUO78371.1"
                     /translation="MDLFRLSRTYRSAKRLQQIVNVLLKHGFGRVIDQIQLGRYIPFR
                     KRLRTFGQWPILKGPTVPERLRIAFGELGPSFIKLAQILSSRPDLITAQFANEFKKLQ
                     DEVPPFPVAEAKKIISEELKVPLEDIFREFKDEPVAAASIAQVHLAKLADGSEVIVKV
                     QRPGIREQIETDITILTALARLLDRNVPESRFFNPVAIVDEFSKTLRRELDFIGEAKN
                     CLRFKKNFEENPDIYIPRIYGELITERVLVMERIEGVRIDDIPGITALGLDRKRLVKV
                     SVDAYFKMILEDGFFHADPHPGNLLVMPSGMLAFVDFGIVGRVTPEMKATMAKTFLAL
                     IHRDFDKLIDQYVELGYVPEDIDLDRFRREFKTDLLYYLEPLYGLSLKEINFAQYLDV
                     ITHLALKHRMQIPSDLLLINKAMLIMENIGRELDPSFDFIAFAEPYAIRLAREKLSPS
                     RMYEHGAKGLEELGDFLVLFPKQIKRIIRKVLRDDIHMKLTHIGLDRLITDMDRSSNR
                     ITFGMIISAILLGSAIMQSTGAGVKIFGMSVLGLLSFALAFLLGIWLLISIIKSGRL"
BASE COUNT          792 a          732 c          850 g          696 t
ORIGIN      
        1 cccatctcct gcaggcatca ctgaggtctg tgctgggtga ccatgtgaag caggcaggct
       61 cttttgtctc gcccgagcgg ttccgttttg actttaccca cttttcgggc cttgaggagt
      121 ctgaagtcgc ttcactcgaa accatggtga atgaaacaat ccttgagaac atcccggtca
      181 acacgtccgt aatggacatc aagagtgcta ttgattccgg tgctatggcc ctcttcgggg
      241 agcggtacgg ggattcggtc cgcgtagttt ctgtgcccgg tgtgagctct gaactttgtg
      301 ggggaaccca ctgcaaggcg acgggagata tcgggctttt cgccatagtc tctgagggaa
      361 gtgtctcgtc aggaatcaga aggatagagg gatttacggg tggtacggcc tttcagtatc
      421 tcaaagaaaa ggccctcgaa ctgaagaaaa tcgatgcatt gttaaaaaca gaccacgcat
      481 acccaaagat agaaagactt ctctctgaac tgaaggcgct cgaaagggag cgggaggagg
      541 tgaaggccaa ggcagcggtg aaggacgcct cggccatcat cgagggggcg cgcagggtga
      601 atgggatagt ggtgatttcc cacagggttg acgatcttga gcagaaggac ctcagaactc
      661 tcgcagacac ccttcgggac aagatcggct cgggtatcat cttcatcacc tcggtaagag
      721 acggccaagc atcaatgctc gcagtcgtca ccaaagacct ggtcggcaga ttcaatgcag
      781 gggagatact taaggaagtt gcgacgatga cagggggtcg cgggggagga aaggcagaaa
      841 tggcccaggg aggcacgaac aagctggata ccctggacag tgcaatggaa tcggtgtacg
      901 gcatcatacg ggagcgctct tctgcggcca tcggatgatc gcccttcatt ctctatcaca
      961 gaagaagcgg agactgatgt tcaatggtct caaaggaggt gtcttatgac tttcttcgat
     1021 gtagtgagaa atgctttgct cgtaggtctt ggtgttcagg aaaaggtgaa tgaattcgtg
     1081 aacgaattgg taaaaaaagg tgaactgagc gaatcccagg gcgcaaagct cctgagggaa
     1141 tggacagaga aggcggataa gggcaccgag cagttcagca agggcctgtc agacatgatt
     1201 tccaagacca tggaaaagat gactctcccg tcaaaggatg acatcgagaa actgaacagg
     1261 aagatccagg ccctctctgt acgcgtcaag aaactagagg gtgtgaaggg ggcggaaaca
     1321 gaagaggaat aacccctgtg gatctcttta gactctcccg cacctaccgg tctgcaaaga
     1381 ggctccagca gattgtaaat gtgctcctca agcacggctt tggccgtgtc atagaccaga
     1441 ttcagctggg aaggtatatc ccgttcagaa agcggctcag aacctttgga cagtggccca
     1501 tattgaaagg tcccactgtg ccggaaagac tgaggattgc ctttggggag cttggtccga
     1561 gttttattaa gcttgcgcag atactctcct caaggcctga cctcatcacc gcgcaatttg
     1621 ccaacgaatt caagaaactc caggatgagg tgccgccctt ccctgtggct gaagcaaaga
     1681 agataatctc tgaagagctc aaggttcctc tcgaagatat attccgggaa tttaaagatg
     1741 aaccagttgc cgccgcgtcc atcgcgcagg tgcatctggc aaaacttgcg gatggctctg
     1801 aggtgattgt gaaggtgcag cgtcccggta tacgggagca gattgagacc gacatcacca
     1861 ttctcaccgc gctggcccgc ctcttggacc ggaatgtgcc agaaagcaga ttctttaatc
     1921 ccgtggctat agtggatgag ttttccaaga cactgaggag ggaactggat ttcattggag
     1981 aggcaaagaa ctgtttaaga ttcaaaaaga atttcgagga gaaccctgac atttatatcc
     2041 ccaggatcta tggtgagctt atcacagaaa gggtcctggt catggagcgg attgaggggg
     2101 tgagaatcga tgacatcccg ggtatcaccg cactgggcct ggaccgcaag agactcgtaa
     2161 aggtgagtgt ggatgcctat ttcaagatga tccttgaaga cgggttcttc catgcagacc
     2221 cccaccccgg aaacctgctc gtaatgccaa gcggtatgct cgcattcgtg gacttcggca
     2281 ttgtgggacg cgtgacccct gagatgaagg ccaccatggc caagacgttc ctcgcactca
     2341 tccacaggga cttcgataag ctcattgacc agtatgtcga actcggatac gttcctgagg
     2401 acattgatct tgacaggttc agaagggagt tcaagacaga ccttctttat taccttgagc
     2461 cattgtacgg tctctctctc aaggagatca actttgcaca gtaccttgac gtgataacac
     2521 acctcgcctt aaagcacaga atgcagatcc cctccgacct cctgctcatc aacaaggcaa
     2581 tgctgattat ggagaatatc ggcagagagc tggacccttc ttttgatttt atcgcttttg
     2641 ccgagcccta tgccatcagg cttgcaaggg aaaagctcag cccgtcaagg atgtacgagc
     2701 acggtgccaa gggcctggag gaattgggtg acttcctcgt ccttttcccg aaacagataa
     2761 agcgcatcat tagaaaagta ctgagagacg acattcatat gaagctgacc cacataggac
     2821 ttgaccggct gatcactgat atggaccgat caagcaacag gatcaccttt ggcatgatta
     2881 tcagtgcaat acttcttggc tctgccataa tgcagtcaac aggcgcagga gtaaaaatct
     2941 tcggcatgtc ggtactgggg ttgctcagtt ttgccctcgc ctttcttctg ggcatatggc
     3001 ttctcatatc catcataaaa tcgggaaggc tctaggtcct gaagcaggtt ctgagggacc
     3061 ttttcatgtt
//
LOCUS       DATKOU010000261        10811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5382001, whole genome shotgun sequence.
ACCESSION   DATKOU010000261 DATKOU010000000
VERSION     DATKOU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5382001"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VMU21_12435"
     CDS             complement(<1..665)
                     /locus_tag="VMU21_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78372.1"
                     /translation="MKKYLAILLGVVFVLGFAASAFAIHAEIPAESQATVAKGSTQIT
                     IGGDIRVRGEYQSNTSKFDNDVADHPTFYDQRVRLSIDAKVTNNTEGFVMIEAANGKQ
                     STGYQWGGNDVPSQGSQGIYQYGDSKRGELNLLEAWIQYKGSGLLGIPAGIKVGHMPL
                     ALGNKVFFDHTLFGDDAIVLFADPTKEMHIVALTAKFMEGNNSNNDDANAYVGVFDYR
                     TKE"
     gene            1147..1704
                     /locus_tag="VMU21_12440"
     CDS             1147..1704
                     /locus_tag="VMU21_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO78373.1"
                     /translation="MKTWKYDFDIAKHTKAIELHPESYGAYNNRGIAYQKKGQHDPAI
                     SDFTKAIRLNPTFVPAYINRGNAHQEVGEYESSVADLNKAIELDPDNSMAYNNRGFTL
                     MLMGRYEDAERDIRRSLELSPNNIYALNSMAELSALRNNAPEACAWLTLAIEKGYNNW
                     SYIRSSRTYDNIRDSLCFKKIISRI"
     gene            1756..2889
                     /locus_tag="VMU21_12445"
     CDS             1756..2889
                     /locus_tag="VMU21_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HUO78374.1"
                     /translation="MPNRSGGKTEFLSALQRAHRIPLKIIHLSILKELVATFLLSVIC
                     LNFILMMEKVLRLSRVLSGVGASLSDMIRIILYLQPQLMILTIPMSLLLSTLLTYGRL
                     NTDNELTILRTAGMSFRHISRPVFILGTASFVMGLLVSFSLGPYGAIQMRKSIATIVT
                     QKAPLAIEAGIFNTSFKDIVILVRDKPAADEMKGIFIYDNRNAQEPKVLVAKEGRVYA
                     DKEYNLSLYMRDGYIHITRNEGSTELFFDGYNLSLNLAMESSSRKNSEMTPFELLALA
                     RKNEPKEGIPFLLEFHRRLSLPSLCLLLMFLGPPLSLLSGKSGRLGGLSLGIGVFTLF
                     YIMLVYGENMARTGVVPHVIGAWTPVLIIAVSSLLAFMKVESR"
     gene            2889..4013
                     /locus_tag="VMU21_12450"
     CDS             2889..4013
                     /locus_tag="VMU21_12450"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HUO78375.1"
                     /translation="MGILQRYYLREFTTLFAIVGIGLGLVFSLMDLINKINDFIPHKP
                     AMADLLLYAGLTLPQYLLYLMPMASLLSGLFVFGQAGKRRETIAIKASGGSMKTLLLP
                     FVYLGIVLSIVSFLMGEFIVPDCSRQTHLLRDTLSKKENILTSKEGTLWLRTNDAIVK
                     IDLFLPGKGTIRGVTIMRMENDMLIERTEAESAEWRPALGSMGSPEAADSHKAGGATR
                     GVWQLRGVTQYTIQSGKITKHKELQTDAIASPEIFSKDVQQPEEMNVRELTAYTQRLK
                     SAGFRNTKLLVDIQSRISYPLINAIMLVLGIALALREVVGGGLVTAAIGIFISLFYWV
                     AYTTSLSLGYTGILPPIPAAWLMPLLFGGMAWHLFRKIPE"
     gene            complement(4119..4778)
                     /gene="deoC"
                     /locus_tag="VMU21_12455"
     CDS             complement(4119..4778)
                     /gene="deoC"
                     /locus_tag="VMU21_12455"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HUO78376.1"
                     /translation="MDKSSVAQLIDHTLLKPDATLAEIRKVCEEAVRFGFHSVCVNPF
                     FLPETTAILSGSGVKVTTVIGFPLGMTLTNVKVYEAMESVLRGSEELDIVMNIGMAKS
                     GKWRDVQKDISDVISATKGVVHKVIIEACYLTREEKGEASRMVLEAGAEFVKTSTGFG
                     PGGATVEDIYLIRSVVKDRCAIKASGGVGSLRQVREFFAAGATRIGTSSGVAIMKEMQ
                     D"
     gene            complement(4874..5764)
                     /locus_tag="VMU21_12460"
     CDS             complement(4874..5764)
                     /locus_tag="VMU21_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HUO78377.1"
                     /translation="MYVYLNDRLVPRAEATVSVFDHGFLYGDGVYETMRVYDDVVFML
                     DEHLMRLFRSATLIGLTIPLDAERIRAAIYDTLSANSLKNAYVRLTISRGYGPIGLDP
                     DLCSTPTFVVIAEEMKDYPKAFYVKGISVIIAKTKRNLSDAINPQIKSLNFLNNILAK
                     IEAKREDAYEAIMLNGSGYLTEGTISNVFFYRDDILCTPSIGCGLLDGITRTVILDLA
                     VREGLMVREGEFGKKDIHAAHEVFLTNTTMEVMPVFQVDEVRYNVGDVGKLLRKAYRE
                     EVNAYVRNMKGRGPSLWGYE"
     gene            5837..6454
                     /locus_tag="VMU21_12465"
     CDS             5837..6454
                     /locus_tag="VMU21_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HUO78378.1"
                     /translation="MKLFPKEIDFFEIFEKAATNLTKATSLLVSLLENFNNIEARAKE
                     IYELEQDGDMLTHDIMKKLNRTFITPIDREDIHALASSMDDILDLIWGGVDRLVVFKI
                     TSPTQEVIEIAKDLHKTTEVVQKTINELRSKNYSHVQEHCIEINRLENRIDRIFRDAL
                     GKLFDDMKDPLLIIKWKEIYEHLEDASDRCEDVANVLESIVLKNA"
     gene            6447..7442
                     /locus_tag="VMU21_12470"
     CDS             6447..7442
                     /locus_tag="VMU21_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HUO78379.1"
                     /translation="MHDTYFLLISVILLATIFDFINGFHDTANAIATSVSTRVLSPKV
                     AVSMAAVLNMVGALSGTAVAKTVGVGLVETYSITQITVVSALISAIVWDIITWYFGLP
                     TSSSHAILSSIVGAAIATAGTGVILQKGIYKVLLGLIISPVIGLALGFLLMVFLIWLF
                     RRSAPSLVSTLFGRLQILSAAYMAFSHGSNDAQKTMGIITMALVSYYKLPDFHVPFWV
                     IVLCATAMAMGTAVGGWRIIKTLGMRLVHLKPIHGFAAETAAATVIEIASRIGLPLST
                     THIISSTIMGVGASKRLSAVRWGIGGNIVLAWILTLPACGLLAWAVCRGLSLIVQ"
     gene            complement(7520..8434)
                     /locus_tag="VMU21_12475"
     CDS             complement(7520..8434)
                     /locus_tag="VMU21_12475"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HUO78380.1"
                     /translation="MVMEDHKLKVFCTVAETKSFSKASEIIHLTQPAVSLQIQALEEL
                     YETKLFDRSSSMVSLTSAGEVLYRYAKEILALYAAAEKTIGELTGLVKGSISVGASST
                     IGNYVLPSVIADFRRTHPKIKIHLFVGNTKRVIELLNSGNIDFGIVEGDVARQKLVVE
                     KLLKDELVVIVPAMHPWAKKKEVSIYDVIKEPFILREEGSGTRQIIEKYFNKHGISTH
                     DMKASMMLGSTVAIKEAVESGMGISIVSRWAARKESKCGSLKLVSIKEENLFREFSLI
                     SQKSAIGSHAVDEFLTYLKGYSFEKLIS"
     gene            complement(8561..9265)
                     /locus_tag="VMU21_12480"
     CDS             complement(8561..9265)
                     /locus_tag="VMU21_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HUO78381.1"
                     /translation="MREDMKGLVEVQGIDSEIARKNAIIEAIPKKVSSVEQPLHEARL
                     LYEKTKQRYEAHVKKKKEKEGLLDEVNEKIKKLRGRTSEIKTNKEYQAHLKEIESGEK
                     EQRGVEDEVLSLMEAVDTAQKELKRDDGKVKIEEEKIEAFKKKLEEDVAEIEKDLDAL
                     KLQREQLVKGIEGELYHLYVQLLETKRGLAVVETRDEVCQGCYMNIPPQLFVEIKKNE
                     KIIQCPQCNRILYWKA"
     gene            complement(9301..9373)
                     /locus_tag="VMU21_12485"
     tRNA            complement(9301..9373)
                     /locus_tag="VMU21_12485"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9338..9340),aa:Ile,seq:cat)
     gene            complement(9487..>10811)
                     /gene="rpoD"
                     /locus_tag="VMU21_12490"
     CDS             complement(9487..>10811)
                     /gene="rpoD"
                     /locus_tag="VMU21_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HUO78382.1"
                     /translation="ERERLFAITEEIKGLYRRRKLYLEHLSRITRERDAFDKKRSGAG
                     KTKKTTGKKGSSKKPKNLGTTLDSQRLKYMRFLEGNREKIVEKVGSLKLKDDVIYVFA
                     EQLERIIQEIEDLNKKMFLLEKRLKRQRGVKTRVQPKGKVSSSKTRKSPSLKNRESLM
                     KKYRDYQEERKELERSRGMGYAEMKRAIKVFDDGRAEVSDAKSEMIEANLRLVISIAK
                     RYIGKGLSFPDLIQEGNIGLMRAVEKFEYQRGYKFSTYATWWIRQAITRALADQSRTI
                     RIPVHMVEVIGRITKATRELVQELGDEPSSEDIADRVNMPVEKVKAIQKITKEPISLE
                     TPIGEEEDSHLRDFIEDKATLSPLDIAINDDLKLQIEKVLCTLNPKEAKIIRKRYGIG
                     EDVPHTLEELGQEFDVTRERIRQIEVKAIRKLKHPARSKWLRTFIESP"
BASE COUNT         2533 a         2917 c         2350 g         3011 t
ORIGIN      
        1 aactccttgg tcctgtaatc aaagacaccg acataagcgt ttgcatcgtc gttgttcgag
       61 ttattcccct ccatgaactt ggcggtgagg gcaacgatgt gcatctcttt cgtcggatct
      121 gcaaaaagca cgatcgcgtc atcaccaaac agggtgtggt caaagaacac cttgttgccc
      181 aatgcaaggg gcatatggcc gaccttgata cctgcaggga tcccgagcag gcctgagccc
      241 ttgtactgaa tccatgcctc aaggaggttc agttctcccc tcttgctgtc gccatactga
      301 tagataccct gagaaccctg gctggggacg tcattcccac cccattgata ccctgtagac
      361 tgctttccgt ttgctgcctc aatcattaca aagccctcgg tgttgtttgt caccttagcg
      421 tcgatcgaca gccttaccct ctgatcataa aaagtagggt gatcggccac atcgttgtca
      481 aattttgatg tgttcgactg gtactcaccc cttaccctga tgtcgccgcc gatggtgatc
      541 tgggtactgc cctttgccac tgtcgcctgg ctttctgccg gtatctcggc atggatggca
      601 aaggcgctgg ctgcaaagcc aagaacgaag actacgccta aaagaatcgc taaatatttt
      661 ttcacttcct aaccctcctg tggttagttc agtattgatc cgtcaaagac ggattgtaaa
      721 ctgatacatt cctgcctcgt ctttgtcttg ttttctcgct ctcaccccct ttttgcacag
      781 attcaggctg catgttcact caaacgcagc ctcacaaatc tgcaggttat tctttgcttt
      841 cgaaaaatat atcacttcat gactccgttg tcaagggaaa aaggcttttc cgtttattta
      901 ttacctacag ataactttgc atagacacaa catacacagt atacagcaat atacatacca
      961 ttgggaaatg cctcgaattt aaaagttttc actagataga ggagtagtga atctgtggta
     1021 attttgcaat agggtgtgca gaagatacac cacatttttt catgggtcgt atttatggat
     1081 tagatatgtt agatattgtt gtattatgct cagacgaaaa ggtaaaatta atccgggtca
     1141 tttcgtatga aaacatggaa atatgatttt gacatcgcga aacacaccaa agccatagaa
     1201 ttacatcctg aaagttacgg ggcgtataac aacagaggaa tagcgtacca aaaaaagggg
     1261 caacacgacc ctgccatttc tgatttcacc aaagccattc ggctgaaccc aacattcgta
     1321 cccgcatata tcaacagggg aaatgcccat caggaggtgg gagaatatga atcctctgtt
     1381 gctgacctga ataaggcgat agaactggac ccggacaaca gtatggccta caataaccgt
     1441 ggattcaccc ttatgctcat gggacgatac gaggacgcgg aaagggacat aagaagatcg
     1501 ctggaactga gcccgaacaa tatttacgca ctgaacagta tggctgagct ttctgcttta
     1561 aggaataatg cgccggaggc ctgtgcatgg ttaactctgg ccattgaaaa gggatacaat
     1621 aactggtcct atatcaggtc ttcaaggact tatgacaata tccgggactc cctctgcttc
     1681 aagaaaatca tatccagaat atgaatgtac ctctcttcaa tcccggggtg ctcaaaacgg
     1741 agaggcacta ccactatgcc taatcgctca gggggaaaaa ctgaattctt gagtgccctc
     1801 caacgtgcac accgaattcc cctgaaaatc atccacctgt cgatcctgaa ggaattagtc
     1861 gccacctttt tgctgagcgt catatgcctg aatttcattc ttatgatgga aaaggtattg
     1921 agattaagca gggtactctc aggggtgggt gcgtccctct ccgatatgat aagaataatc
     1981 ctttatcttc agccccagct gatgatactc acgattccca tgtcactcct cctctccacc
     2041 ctgctcacct acgggagact caacacagac aacgaactca ccatcctcag gactgcgggg
     2101 atgtctttca ggcatatttc ccggccggta ttcatcctgg ggaccgcctc ctttgtcatg
     2161 ggcctgctgg taagtttctc gctgggcccg tacggggcga tccagatgag gaaaagcatc
     2221 gcaaccatcg tgacccaaaa ggcccccctg gccatagagg caggcatatt caacacctcc
     2281 ttcaaggaca tcgtcatcct ggtacgggac aaaccggcag ctgatgaaat gaaaggaata
     2341 ttcatctatg acaacaggaa tgcacaggag ccgaaggtcc tcgtggcaaa ggaaggcagg
     2401 gtatacgctg ataaggaata caacctttct ctctacatga gggacgggta tatccacatc
     2461 acaagaaacg aaggctcgac cgaactattt ttcgacgggt acaatctttc attgaatctt
     2521 gccatggaaa gctcctcgag gaagaacagt gaaatgacac cctttgagct cctcgcactg
     2581 gcgaggaaaa atgagccaaa ggaggggatt cccttccttt tggaatttca caggcggctc
     2641 tcccttccct ctctctgtct gctcctcatg ttccttggtc cgcccctttc cctgctctcg
     2701 ggaaaatcgg gcaggctcgg aggactttct ttgggtattg gcgtcttcac tctcttttac
     2761 ataatgctcg tgtatggaga gaacatggcg agaacagggg tcgtccccca tgtgataggg
     2821 gcatggacac cggtacttat catcgcggta tcgtccctcc tcgcgtttat gaaggtggag
     2881 tcaaggtaat gggaatactt cagagatact atctcagaga attcactacg ctctttgcca
     2941 ttgtcggcat cggtcttggt cttgtgttca gtctcatgga tcttatcaac aagatcaacg
     3001 acttcatacc ccacaaacct gccatggctg atctcctcct ctatgcaggt ctgaccctcc
     3061 cgcaatatct gctgtatctc atgcccatgg catccctctt gagcggtctg ttcgtctttg
     3121 gccaggcagg gaagaggaga gagaccattg ccatcaaggc ttccggagga agcatgaaaa
     3181 cccttctcct cccctttgtg tatctgggaa tagtgttgag tattgtgagt ttcctcatgg
     3241 gggagtttat cgtccctgat tgttcgagac agacacatct cctcagggat accctctcaa
     3301 agaaagagaa tatactcacc tccaaggagg gtacactctg gctccggacg aatgatgcga
     3361 tcgtaaaaat tgacctcttc cttcccggta aagggaccat caggggcgtc accatcatga
     3421 gaatggaaaa tgatatgctc attgagagaa ctgaggcgga gtccgccgag tggaggcctg
     3481 cgctgggctc catggggtct ccagaggctg ccgatagcca caaggccgga ggtgcgacga
     3541 ggggagtctg gcagctcaga ggggttaccc agtacacgat ccaatcgggg aaaatcacga
     3601 aacataaaga gctgcagaca gatgctattg cttcaccgga gatcttcagc aaagacgtgc
     3661 agcagccgga ggagatgaac gtgagggaac tcactgcgta tacacaaagg ttgaagagcg
     3721 cgggctttag aaacaccaag ctccttgtgg atattcagtc gaggatttcc tacccgctca
     3781 ttaacgcgat tatgctcgta ctcggaatcg ccctcgccct cagggaggtt gtgggtgggg
     3841 gactcgtgac cgcagccata ggtatattca taagcctctt ctattgggtc gcgtacacca
     3901 catccctttc gttgggatac acgggaatcc tccctccaat acccgccgca tggctcatgc
     3961 cgcttctttt cggcgggatg gcatggcatc tcttcaggaa aattccggaa taacaggccc
     4021 tgaccggttc ttcgggaagg agaacccctc agcgtttctc cgggactcca attcgggatc
     4081 gcgaatcttg ttattatgga gatcatccat tggtaatttc aatcctgcat ttctttcata
     4141 atcgcaaccc ccgacgatgt gcctatcctt gttgcccccg cagcaaagaa ttccctcacc
     4201 tgccttagag accctactcc ccctgacgcc ttaatggcgc acctgtcttt taccactgat
     4261 cgaataaggt aaatatcctc caccgtcgca ccgccgggac cgaatcctgt agaggtcttc
     4321 acaaattccg cccctgcttc aagaaccatt cgggaagcct cacccttttc ctccctcgtc
     4381 agatagcatg cctcgataat tactttgtgg acaactccct tcgtcgccga aattacatca
     4441 gagatgtctt tctgaacatc ccgccatttt cctgatttcg ccatccctat attcatgacg
     4501 atgtcgagtt cctcgcttcc gcgtaaaaca gactccattg cctcatacac cttcacgttg
     4561 gtaagcgtca tacccaaagg aaatcctatt accgtcgtga ccttcacccc gcttcccgaa
     4621 aggatagcgg ttgtctctgg cagaaagaat ggattcacgc ataccgaatg aaatccgaat
     4681 ctgaccgctt cttcacatac ttttcttatt tcagctaaag ttgcatctgg cttcaggagg
     4741 gtgtgatcaa ttaattgtgc aacgcttgat ttatccattt tttcttcgtg aagctcatag
     4801 gtccctgtgt ccgaacgctt tgaaagaagg catgatacct accattcatc cccttgtact
     4861 ttcctgccga ttcttactcg tatccccata aggatggtcc tcttcccttc atattcctta
     4921 cgtaggcatt gacctcctcc cggtaagcct tccgcaaaag ctttcccaca tctcccacgt
     4981 tatatctcac ctcatccacc tggaagaccg gcattacctc catagtggta ttggtgagaa
     5041 acacttcgtg tgcggcatgg atatccttct tgccgaactc gccctccctc accataagcc
     5101 cttccctcac tgcgaggtca aggataactg ttctcgtgat cccgtcaagg agtccgcagc
     5161 cgatggacgg cgtacagaga atgtcatcac ggtaaaagaa gacgttgctg atggtccctt
     5221 cggtgagata tcctgagcca ttgagcatta tggcctcgta cgcgtcttct ctctttgcct
     5281 ctatctttgc aaggatatta ttaagaaaat taagagactt gatctgtggg tttatggcat
     5341 cgctcaaatt cctttttgtc tttgcgatga tgacgctgat ccctttcaca tagaatgcct
     5401 tcgggtaatc cttcatctct tcagcgatca ccacaaaagt cggtgtgctg cacaggtcgg
     5461 ggtcaagccc gatgggcccg tatccgcgag aaatggtgag tctcacatac gcattcttca
     5521 gggaatttgc actgagggta tcatagattg ccgccctgat cctctccgcg tccaaaggga
     5581 tggtgaggcc tatgagggtg gcagagcgga aaagcctcat aagatgctca tccagcataa
     5641 agaccacatc gtcgtatacc ctcatggtct catacacacc gtcgccgtac aggaagccat
     5701 ggtcaaagac cgagaccgtg gcctccgctc tcggaacaag cctgtcattc aggtacacat
     5761 acatcgctga aattatacca ttaactcccc ttatttgact ttctttcctg attcgtatat
     5821 atagtaaaca ttcggaatga aactctttcc aaaagaaatc gattttttcg agatttttga
     5881 gaaggctgcc accaacctca caaaagccac ctccctcctc gtttcgctcc tggagaattt
     5941 caacaacatc gaagcaaggg caaaggagat ttatgaactc gagcaggacg gagacatgct
     6001 cacccacgat atcatgaaga aactcaacag gacgtttatt actcccatcg acagggaaga
     6061 catccatgcc cttgcatcga gtatggatga cattctcgac cttatatggg gtggggttga
     6121 caggcttgtg gtgtttaaaa tcacctcacc tacccaggag gtgattgaga ttgctaagga
     6181 tctccacaag accactgaag tggtgcaaaa aaccatcaat gagctgcgct caaaaaatta
     6241 ctctcacgtt caggagcatt gcatcgagat caataggctc gaaaacagga ttgacagaat
     6301 cttcagggac gccttgggaa agctctttga tgatatgaaa gatcctctcc tcatcatcaa
     6361 gtggaaagaa atttacgaac acctcgaaga tgcatccgac cggtgtgaag atgtggcaaa
     6421 tgttttagaa agtatagtcc tcaaaaatgc atgacaccta cttcctgctc atctcggtaa
     6481 tacttctcgc aacgatcttt gatttcataa acggttttca cgacaccgcc aatgccatcg
     6541 ccacatccgt ctcaacacgg gttctgtccc cgaaggtggc ggtatccatg gcagcagtgc
     6601 ttaacatggt gggtgcgctt tcgggaaccg cggtggccaa aacagtgggg gtgggccttg
     6661 tggaaaccta ctccatcaca caaatcaccg tcgtttccgc ccttatctcc gccattgtat
     6721 gggatatcat tacgtggtat ttcgggcttc ccacgtcctc aagccatgcg atcctctcaa
     6781 gcatcgtggg agctgcgatc gctactgcgg gaaccggcgt catccttcaa aaagggatct
     6841 ataaggtact tctcggcctg atcatttctc ctgtcatcgg attggccctc ggattcctcc
     6901 tcatggtatt cctcatctgg ctgttcagga gatctgcccc ttcgctggtg agcactctct
     6961 ttggcaggct tcagatcctt tcagcggcat atatggcctt cagccacgga agcaacgatg
     7021 cccaaaagac catgggcatt atcaccatgg ccctggtaag ctattataaa ctccctgact
     7081 ttcatgtgcc tttttgggtc atcgtcctct gcgcaacggc aatggccatg ggaaccgcgg
     7141 tagggggatg gaggatcatc aagaccctgg ggatgcgtct tgtgcatctc aagcccattc
     7201 acgggtttgc tgccgagacc gcggcagcca cagtgataga gattgcttcg agaataggcc
     7261 ttcccctttc gaccactcat atcatttcct ccacgatcat gggagtaggg gcatcaaaga
     7321 ggctctcggc ggtgagatgg ggaataggag ggaatatcgt gctcgcatgg atccttacac
     7381 tgcctgcatg tggacttctt gcatgggcgg tgtgcagggg gctaagtctg attgttcaat
     7441 aacgcttgtt gctctcctcc cgtgccagga gacctttatc cgttggacta taccattcag
     7501 gactatgaat cgtatctggc tacgaaatga gtttttcgaa agagtaaccc ttaaggtagg
     7561 ttaagaattc atccactgcg tgggatccga tcgcgctctt ctgggatatc aaagagaact
     7621 ccctaaagag gttttcctcc ttgatactca ctaatttaag gctcccgcac ttgctctctt
     7681 tcctcgccgc ccaccgcgag acaatggaga tgcccatgcc gctctccacc gcttccttga
     7741 tcgccacagt gcttcccagc atcatggatg ccttcatgtc atgggtactt atgccgtgtt
     7801 tgttaaaata tttctctatg atctgccttg ttcctgaccc ctcctccctc aggataaatg
     7861 gttccttaat cacgtcataa atggagacct ctttcttctt cgcccatgga tgcatcgcag
     7921 ggacgatcac cacaagttcg tctttgagga gcttttccac tacgagtttc tgccttgcca
     7981 catcaccttc aacaatcccg aaatcgatat tccccgagtt caagagttct ataaccctct
     8041 tcgtattccc tacaaagaga tgtatcttga tcttcggatg tgttctccga aaatcagcta
     8101 tcacagaggg caaaacataa ttgccgatgg tcgagctcgc ccccacgctt atacttccct
     8161 tgacaagacc tgtcagctcc ccgatagttt tttccgctgc tgcatagagg gcgaggatct
     8221 cctttgcata ccgatagagg acctctccag ctgaggtgag ggacaccatg ctcgatgatc
     8281 ggtcaaacag tttcgtctcg tagagttcct caagggcctg tatctgtaaa ctgaccgcgg
     8341 gttgggtgag gtggatgatc tcggatgcct ttgaaaagct ctttgtctct gcgacggtgc
     8401 agaacacttt tagtttgtga tcttccataa ccattagttt tgcttattgt actaattatt
     8461 tttttaatag tcaagtaatg cggtgggcat gccttttcgg tcaaatcctg gatatctcct
     8521 tcttttgtga gcgttgcaac gggtgagagc cctcttcttg tcaggctttc cagtaaagta
     8581 tccgattaca ctgcgggcat tgaataatct tttcgttctt ctttatttct acaaagagct
     8641 ggggaggaat attcatataa catccttggc atacctcatc cctggtttcc accacggcaa
     8701 gccccctttt tgtctcaagg agctggacat aaagatgata gagttcgccc tcaataccct
     8761 tcacaagctg ttccctttga agcttgagcg catccagatc tttttcgatc tccgcaacgt
     8821 cctcctccaa cttcttctta aaggcctcga tcttctcttc ctcaatcttg acctttccat
     8881 cgtctctctt tagctccttt tgtgccgtat ccactgcctc catcaaggac agcacctcat
     8941 cctcaactcc ccgctgttct ttttctcccg actctatctc cttcagatgc gcctgatatt
     9001 ccttattcgt ttttatctct gatgtccggc ccctcagttt ctttatcttt tcatttacct
     9061 cgtccaatag cccttccttc tctttcttct tctttacatg cgcttcatat ctctgttttg
     9121 ttttttcata caacaatcgt gcttcatgta atggttgctc aacggatgag accttcttgg
     9181 gaatggcttc aatgatcgcg ttctttcttg caatctcgga atctatcccc tgaacctcta
     9241 caagcccttt catgtcttct ctcacacacc ctccacccta ctcgcctgcc aattttttaa
     9301 tgggcccacc aggactcgaa cctgggacca accggttatg agccggtagc tctaccaact
     9361 gagctatggg ccctcataag aacttcttac actgcggaca tcgcactcca gacctgggat
     9421 gagcctcttg aaagactgag ggctatgccc cggctctttt actataaact cgcaatgttt
     9481 tttcggtcaa gggctttcaa taaaagtcct taaccatttg ctgcgtgccg gatgtttaag
     9541 ttttcggatg gccttaactt ctatctgtct gatccgctcc cttgttacat caaactcttg
     9601 tccaagttct tcaagggtat gggggacatc ttcgcctatg ccgtatctct ttctaataat
     9661 cttcgcctct ttcgggttaa gggtgcagag caccttttct atctgaagtt taagatcgtc
     9721 gttgattgca atgtcaaggg gggaaagggt tgccttgtct tcaataaagt ccctgagatg
     9781 gctgtcctcc tcctctccta tgggggtctc aagagatatg ggctccttcg tgatcttctg
     9841 tattgccttc actttttcca ctggcatgtt caccctgtcc gcaatgtcct cagaagatgg
     9901 ctcatctccc agctcctgta cgagttccct tgtggccttc gtgatacgtc ctatcacctc
     9961 caccatatgc acaggaatcc ggattgttct tgactggtca gccagggccc ttgtaatcgc
    10021 ctgcctgatc caccaggtgg cgtaggtgct gaatttatac cccctctggt attcaaattt
    10081 ctctaccgcc ctcattaatc cgatgttgcc ctcctgaata agatcgggaa aactcagccc
    10141 ctttccaatg tatctctttg caatactgat cacgagcctg agattggcct ctatcatctc
    10201 actcttcgca tcagatacct ccgccctgcc atcatcaaat acctttattg ccctcttcat
    10261 ttcggcatag cccattcctc gtgagcgctc cagttctttt ctctcctcct ggtaatcccg
    10321 gtactttttc attagggact ctctattctt taaagaagga gattttctgg tctttgagga
    10381 actgaccttc ccttttggtt gcacccttgt tttcacgccc ctctgcctct taagtctttt
    10441 ctccaataaa aacatcttct tattgagatc ttcgatctct tgtattatcc tctccagctg
    10501 ttcggcaaag acatatatta catcgtcttt aagctttagg cttcccacct tctctactat
    10561 tttctccctg ttcccctcca ggaacctcat gtatttgagt ctttgggagt caagggtcgt
    10621 cccgagattc tttggtttct tactagatcc ctttttcccc gttgtcttct tcgtttttcc
    10681 cgctcccgac cgcttcttgt cgaatgcgtc cctctcccgg gtgatcctgg agaggtgctc
    10741 gagataaagc tttcttctcc gatacaatcc ttttatctct tcggtaatgg caaagagcct
    10801 ttccctctcg t
//
LOCUS       DATKOU010000262         7743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5757174, whole genome shotgun sequence.
ACCESSION   DATKOU010000262 DATKOU010000000
VERSION     DATKOU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5757174"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2471
                     /locus_tag="VMU21_12495"
     CDS             <1..2471
                     /locus_tag="VMU21_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUO78383.1"
                     /translation="GTLEGRQQAFTIQATGQLFNAEGYRPLIVAYRNGKPVRLEELGR
                     VIDSVENNKVANWYNDDRAVVLAVQRQPGTNTVEVVDSIKKLLPVFRVQIPPSVNLNI
                     LYDRSVSIRESVNDVKVTLFLALCLVVLVIFLFLRNLSATIIPSLALPFSIIGTFAGM
                     YLMGFSVDNISLMALTLSVGFVVDDAIVMLENIVRHIEKGENVFEAALRGSREIGFTI
                     LSMTLSLVAVFIPVLFMGGILGRILHEFAVTISMAILISGFVSLTLTPMLCSRFIRPH
                     SAAERHGRLYMFMERFFQGMLHTYERTLKLVLDYRLTTLAVTIILTVATGFLFTKIPM
                     GFLPSEDTGSIFAFTEAAQGISFEDMVKHQQKLAEIVGKNPNVDSFMSAVGASGISVA
                     NNTGRIFMRLKPRSERKMSADEIIQELRPKVSTMPGIQMFMQNLPAIRIGGQLTKSQY
                     QFTLQSPDPDELYRRAAELEGKMRALTQLQDVTSDMQIKNPQVNIEINRDKASALGVT
                     ATQIEDALYYAYGSRQVSTIFAPNNQYQVILELEPKYQGDPSALSLLYIRSDKGQLVP
                     LNTVANLTQNIGPLTVNHLGQLPAVTISFNLKPEVALGDAVTVVDKLAHATLPQTIST
                     SFQGTAQAFQSSMKGLWILLIMALLVIYIVLGILYESFIHPLTILSGLPAAMFGALVT
                     LLLFHKDLNIYAFVGVIMLLGIVKKNAIMMIDFALEAQRTQGKAPLDAIYEGALVRFR
                     PIMMTTMAALMGTLPIAIGFGAGADARRPLGLAVVGGLVVSQLLTLYITPVVYYYMDR
                     FQQKVSALLGKRKKAKGESMVQTG"
     gene            2613..3944
                     /locus_tag="VMU21_12500"
     CDS             2613..3944
                     /locus_tag="VMU21_12500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUO78384.1"
                     /translation="MRSFITLIICMACFLPVRGGAEEIIQKGELLSLQRCIEIGLMKN
                     PTIMAAKGTIGVNESLVGQAKSNYYPQVSWTGGYSRILPVSGRSFTGTQGGTFGTGDQ
                     GTSSSSSAFDQYTTSFSLSQLIYDFGKTSTQVQISRLNTDASRSTFENTSEQVILSVK
                     QTYYAVLQAKYNKLVLADTVKQTEQHLEQAKGFYEVGTKPKFDVIKAEVDVSNAKLNL
                     IKAENDFRIAVVNLNNAMGIPDAPDYTLDENLSFEKYGTTFEEALSKAYENRPDLKSI
                     VAQRQAAEVGIDSAKTGYYPLLTGNASYNWAGERIDQMDHGWNVGAVISFPIFSGFLT
                     KYQVQQARANLNVLKANEESLRQTVFLEVQQAYLTLRAAEEAIPTAKLGVGQAQENLD
                     IANGRYAAGVGNPVEVTDAEVSLANARLQYIQALYADKVAQANLEKAMGMR"
     gene            3941..5104
                     /locus_tag="VMU21_12505"
     CDS             3941..5104
                     /locus_tag="VMU21_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUO78385.1"
                     /translation="MRKILIVGAGILVIVAAVAAFLLFGNKAGETKYKTEKVTKGDII
                     TSVTATGTVNAVTTVLVGTQVSGTISKIYVDFNSPVKKGQLIAQIDPALFEAQVAQSK
                     ANLVAAQANVEKADATLADAKRTRERNAELFSKNLIAKSDFDTADTNYETAKASVNAA
                     KAAVAQADAALKNSQTNLGYTRIVSPVDGTVVSRNVDVGQTVAASFQTPTLFTIAQDL
                     TKMQIDTSVDEADIGKVKVGQEVEFTVDAYADTTFKGKVGQVRIAPITVQNVVTYDVV
                     ITVDNPDIKLKPGMTANVSIIVAEKKDTLKIPNAALRFRPAGMTKTKQSQKGAGVWTL
                     EKGKPKRLPVNIGISDGNFTELLAGELREGQDVVIEETVSKTKGPAPAGPRMF"
     gene            5106..5798
                     /locus_tag="VMU21_12510"
     CDS             5106..5798
                     /locus_tag="VMU21_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUO78386.1"
                     /translation="MSLIEVRELSKVYRLGDIEVNALMDVSTTIAKGEFVAIMGPSGS
                     GKSTFMNVLGCLDQPTGGQYLLEGIDVGKLNRDELAGIRNKKIGFVFQGFNLLSRTSA
                     MENVEIPLLYDGHHARQRRERAMAALKSVGLEGREHHHPNQLSGGQQQRVAIARALVN
                     DAPIILADEPTGNLDTKTSVEIMELFVKLNTESSITIILVTHEPDIATYSRRQIKFLD
                     GRIVSDEMTGNA"
     gene            5795..7024
                     /locus_tag="VMU21_12515"
     CDS             5795..7024
                     /locus_tag="VMU21_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO78387.1"
                     /translation="MINLPSTFRISLRALRVNKMRSALTMLGIIIGVGAVIAMLAVGT
                     GASRKIAEQIASMGSNLLIILPGSTTSGGVRMGAGTQPTLTLNDAEEIQKESSAVAEV
                     APVLNGVAQIVYGHQNWSTGVTGTTPGMLEVRDWPLASGRPFTQQDVKSATKVCLLGQ
                     TVVDNLFGSMDPLGQIIRIKKIPFTVIGVLATKGQSAQGQDQDDTIYVPVTTAQKKLF
                     GTSFPGMVRTIMVKAKSAEDLGPAEKQINELLRQRHHIGPKQDNDFTVRNLTQIMQTA
                     EQSTKVMTLLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRLQF
                     IIEALTLSLIGGIIGIIIGVSGSKILSSLAGWATIVSPLSVFMAFGFSGLVGIFFGFY
                     PAYKASNLNPIDALRFE"
     gene            complement(7323..>7743)
                     /locus_tag="VMU21_12520"
     CDS             complement(7323..>7743)
                     /locus_tag="VMU21_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUO78388.1"
                     /translation="AAVAPLGNPRGFIPVDGNYRHTKYKNIFSVGVAVAIAPPEQTPV
                     PTGVPKTGYMTVKMAKAASLAIVSDITGSVPPDEIPMEVLCLMDMGGTAVYMKASPVV
                     PPRQDSDLREGIMYKWMKIGFERYFLYKMKHGFSNWP"
BASE COUNT         1933 a         1946 c         2117 g         1747 t
ORIGIN      
        1 caggtacact ggagggaagg caacaggcat ttaccataca ggcgaccgga cagcttttca
       61 atgcagaagg ctaccgtccc cttatcgttg cctatcggaa cggcaagcca gtccgcctgg
      121 aagaactggg cagggtcatc gacagcgtcg aaaacaacaa ggtggcgaac tggtataacg
      181 atgaccgcgc agtggtgctg gccgtccagc gtcagcccgg caccaacact gtggaagtag
      241 tggacagcat caagaaactg ctgcccgtct tccgtgtcca gataccccct tcggtcaacc
      301 tgaacatcct ttatgatcgc tcagtatcca ttcgtgagtc tgtcaacgac gtcaaggtta
      361 cacttttcct tgccttgtgc ctcgtggtgc tggttatttt tctcttcctg cgcaacctct
      421 ctgctaccat tatcccgagc cttgcactcc ctttctccat cattggcact tttgcaggca
      481 tgtacctcat ggggttcagc gttgataata tctccctcat ggccctgacg ctctcggtgg
      541 ggtttgtggt ggacgatgcc atcgttatgc tcgagaacat tgtgcgccat atagagaaag
      601 gggagaatgt ttttgaggct gcactccggg ggtcaaggga aatagggttc accattttat
      661 ccatgaccct ttcccttgtg gcggtcttta tcccggtgct cttcatgggc gggatccttg
      721 gccgtattct ccacgaattt gcagtgacca tcagcatggc gatcctgata tcagggtttg
      781 tctccctgac ccttaccccc atgctttgca gccgcttcat ccggccgcat tcggctgctg
      841 aacggcacgg ccgcctctat atgttcatgg aacgcttttt ccagggaatg ctgcacacct
      901 atgaacgcac tctcaagctg gtccttgact atcgcctgac caccctggca gtcactatta
      961 tccttactgt ggcgacaggt tttctcttca cgaaaattcc catggggttc ctgccatcgg
     1021 aagatacggg gtcaatcttt gcctttactg aagctgccca gggcatttcc tttgaagaca
     1081 tggtgaaaca ccagcaaaaa ctggcggaga ttgttggcaa gaaccctaat gtggattcct
     1141 ttatgtccgc tgtaggggcg agcgggatca gtgtggcaaa caatacaggc cgcattttca
     1201 tgcgtctgaa accaagaagt gaacggaaaa tgagcgcgga cgaaattatt caggagctgc
     1261 ggccaaaggt gtcaacgatg cctggtatcc agatgttcat gcaaaacctt cctgccatcc
     1321 gcatcggagg ccagctgacc aagagccagt accagtttac cctgcagagc cccgaccctg
     1381 acgagcttta ccggagggct gccgaactgg agggtaagat gcgggcattg acgcaactcc
     1441 aggatgtgac cagtgacatg cagatcaaga acccccaggt gaatatagag ataaaccgcg
     1501 acaaggcctc cgcgcttggt gtcaccgcga cgcagattga ggatgccctc tattacgctt
     1561 atggctcccg ccaggtttcg accatttttg ctcccaacaa ccagtaccag gtgattctgg
     1621 aactggagcc gaagtaccag ggggatccct cggccctgtc attgctctat atccgatcag
     1681 acaaggggca actggtgccg ttgaatactg tggcgaatct gacccagaac atcggtcctc
     1741 ttacagtgaa tcaccttggc cagcttcctg cagtaactat ctcctttaac ctcaagccgg
     1801 aagtggcact gggggatgca gtgacggtgg ttgacaaact ggcgcacgcg accctgcctc
     1861 aaaccataag caccagcttc caggggaccg cccaggcgtt ccaatcttcg atgaaaggac
     1921 tctggatact gcttatcatg gccctcctcg tgatttacat tgtgctggga atcctctacg
     1981 agagcttcat ccatcccctc accatccttt caggcctccc cgctgcgatg ttcggagcat
     2041 tggtcaccct gttgctcttt cacaaggatt tgaatatcta tgcctttgta ggagtgatca
     2101 tgctgctagg gatagtcaag aagaatgcga tcatgatgat cgactttgcg ttggaggcac
     2161 aacggaccca aggaaaggcg cccctggatg caatctatga aggtgcgctc gtccgtttcc
     2221 gtcctatcat gatgaccacc atggcagcac ttatggggac tctgcccatc gccatcggtt
     2281 tcggcgccgg agctgatgcc cgccgccctt tgggtctcgc ggttgtgggt ggactggtgg
     2341 tttcccagct tcttacgctt tatataactc ccgtggtgta ttactatatg gacagattcc
     2401 agcagaaggt gagtgccttg ctcggaaaaa ggaagaaagc taagggtgag agtatggtac
     2461 agacagggta gtatggtgat tctgtcccca cttgctaaga tacggagtcg tgacgggcat
     2521 ctaaaccctt tttatggaca aggcgtctac ttggtagtat ctttttgcat tttgaaacag
     2581 catgaattga ataaaggttc aggaggacat ttatgaggtc atttattacg ctcattatat
     2641 gtatggcatg ttttcttccc gtgagaggag gggcggaaga gatcattcag aaaggagaac
     2701 tgctcagtct gcagcgctgc atcgaaatcg gcctgatgaa aaaccccacc attatggctg
     2761 caaaaggtac gataggtgtg aatgagagtc tggttgggca agcgaagtcg aattactatc
     2821 cccaggtaag ctggacaggc ggatatagca ggatattgcc ggtctccggc cgctccttca
     2881 ccggtactca gggaggcacc ttcggaacgg gtgaccaggg cacttccagt tcgtccagcg
     2941 catttgatca atacacaacc agcttctctc tcagccagct catctacgac tttggaaaga
     3001 cctcgacgca ggtccagata tcacgactga atactgatgc ttcccggtcg accttcgaaa
     3061 acacgtcgga gcaggtaatc ctgagcgtta aacagacata ttacgcggtc ttacaggcaa
     3121 agtataacaa gctggttctc gcagataccg tgaagcagac cgagcagcac cttgagcagg
     3181 caaagggctt ttatgaggtg gggacgaaac ccaaatttga tgtgataaag gcagaggttg
     3241 acgtgagcaa tgcaaagctg aatctcataa aggccgaaaa cgacttcagg atagcggtgg
     3301 tgaacctgaa caatgccatg ggcatccctg atgcacccga ctatacgctc gacgaaaacc
     3361 tctcttttga gaaatacggg acgaccttcg aagaagctct ctcaaaggcg tatgaaaaca
     3421 gacctgacct gaaatccatc gtcgcccagc ggcaggcggc tgaagttggg atcgactccg
     3481 caaagaccgg ttattatccc ctgctgaccg gtaacgcttc gtataactgg gcaggggaaa
     3541 gaattgacca aatggatcat ggctggaatg tgggtgcggt catctctttt cccatcttca
     3601 gcggctttct gaccaaatac caggtgcagc aagcgagagc gaacctgaat gtcctcaagg
     3661 cgaatgaaga gtcgttgagg caaaccgtgt tccttgaggt gcagcaggca tatctcactc
     3721 tcagggctgc agaagaggcg attcccactg caaagctggg cgtggggcag gcgcaggaga
     3781 acctggacat cgcaaacggc aggtatgcag caggggtggg gaaccccgta gaggtgaccg
     3841 atgctgaggt atcgctggcg aacgcacgat tacaatatat ccaggcgctc tatgctgata
     3901 aggttgccca ggcaaatctc gagaaggcca tggggatgag atgaggaaga tactcattgt
     3961 tggcgctggt attcttgtca tcgtcgcagc cgttgcagca ttccttctct ttgggaacaa
     4021 ggcaggcgag acgaagtata agaccgagaa ggtcaccaag ggtgatatca tcacctccgt
     4081 gactgcaaca gggacggtga atgccgtgac cacggtcctt gtgggtaccc aggtatccgg
     4141 gacgataagt aaaatatatg tggatttcaa ttcgccggtg aaaaagggac agttgattgc
     4201 ccagatcgat cctgcattgt ttgaggcaca ggttgcccag tcaaaggcga atctcgttgc
     4261 tgcgcaggca aacgtggaga aggccgacgc aacgcttgcg gatgcaaaga gaacaaggga
     4321 gaggaacgca gaactcttct cgaaaaacct tattgcaaag agcgatttcg ataccgcgga
     4381 cacgaattat gaaacggcaa aggcatcggt gaacgcggca aaggccgcag tggcccaggc
     4441 agatgccgca ttgaaaaatt cacagaccaa tctcggatac acaaggatag tttctcccgt
     4501 ggacgggact gtcgtatcga ggaatgtgga tgtgggtcag accgtggcgg caagcttcca
     4561 gacaccgact ctgtttacca tagcgcagga cctcacaaag atgcagatcg ataccagtgt
     4621 ggacgaagca gacatcggca aggtgaaagt gggacaggaa gtggaattca cggtagatgc
     4681 atatgcggac accaccttta aggggaaggt ggggcaggta aggattgccc ccataaccgt
     4741 acagaacgtg gtcacctatg acgtagtgat caccgtggac aaccctgata taaaactgaa
     4801 gccaggcatg accgccaatg tatccatcat tgtcgcggaa aagaaagaca ccctgaaaat
     4861 ccccaacgcc gcgctcagat tcagacctgc ggggatgaca aaaacgaagc agtcccagaa
     4921 gggcgcaggc gtctggacac tagaaaaggg gaaacccaag cgtttgcctg taaacatcgg
     4981 cataagtgac gggaatttta cagagctttt ggcaggagaa ctcagggaag gtcaggatgt
     5041 ggtcatagaa gaaacggtgt caaaaacaaa aggaccggcg cctgcgggtc caagaatgtt
     5101 ctaacgtgtc tcttatcgaa gtgcgtgaac tcagcaaggt ctacaggctc ggagatattg
     5161 aggtcaacgc gctcatggat gtctcgacaa ccatcgcaaa aggtgaattc gtggcaatca
     5221 tgggaccctc gggctcgggc aagtcaacct tcatgaatgt cctgggttgt cttgatcagc
     5281 ctacaggagg acagtatctc cttgagggca ttgatgtggg caaactcaac agagacgagc
     5341 ttgcaggcat acggaacaag aagatcggct tcgtgttcca ggggttcaat ctcctttcac
     5401 ggacatcggc catggagaac gtggagattc ccctgctcta tgacgggcac catgcaaggc
     5461 agagaagaga gcgggccatg gcagccctga aaagcgtggg tctggagggg agggagcatc
     5521 accacccgaa ccagctttca gggggccagc agcagcgggt tgcgatagcg cgcgcccttg
     5581 tgaatgatgc gcccataatc cttgcggatg aacctaccgg caacctggac acgaagacga
     5641 gcgtcgagat catggagctt tttgtaaagc tcaacaccga gtcgagtata accataatac
     5701 tggtgactca cgagccggat atcgcaacct acagcaggcg ccagattaaa ttcctggacg
     5761 gtcgtattgt gagcgatgaa atgacgggga acgcatgatc aatcttccct ccacattcag
     5821 gatatccctg agggcgctca gggtgaacaa gatgcgatct gcccttacca tgctcggaat
     5881 tattatcggg gtgggagcgg taatcgccat gcttgcggtc ggcaccgggg cgagcaggaa
     5941 gatagccgag cagattgcga gcatgggaag caatctcctt atcatccttc cgggttcgac
     6001 cacttccgga ggggtgagga tgggagcggg cacacagccc acccttaccc ttaatgacgc
     6061 ggaagagata cagaaggaga gttctgcagt ggccgaggtt gcccctgtgc tcaacggagt
     6121 cgcacagatc gtgtacggcc atcagaactg gtcaaccggt gtgacgggga caacaccagg
     6181 gatgctggag gtgcgggact ggccacttgc ctcgggaaga ccctttaccc agcaggatgt
     6241 gaaaagcgct accaaggtct gtcttttggg acagaccgtg gtggacaatc tcttcgggag
     6301 catggatcct ctgggccaga tcataaggat taagaagatc ccttttactg tcatcggtgt
     6361 tctggcgaca aaggggcagt cggcccaggg ccaggaccag gacgatacca tctatgtccc
     6421 agtgaccacg gcacaaaaga agcttttcgg gacctctttc ccgggaatgg taaggaccat
     6481 tatggtgaag gcaaagagcg ctgaagacct cggacctgcc gaaaagcaga ttaatgaatt
     6541 acttagacaa aggcatcaca tcggcccaaa acaggataat gattttacgg tccggaacct
     6601 gactcagatc atgcagaccg cggagcaatc cacaaaggta atgacactct tgctgggtgc
     6661 tattgcgtcg gtctctcttc ttgtgggagg aataggcatc atgaacatca tgctcgtctc
     6721 ggttaccgag cggacaaggg agatcgggat acgcatggca gtgggtgcaa agacatggga
     6781 catcaggctc cagttcatta tcgaggcact caccctgtca ttgatcggag gcattatcgg
     6841 aattatcatc ggggtgtctg gctcaaaaat actctcgtcc cttgcagggt gggcgaccat
     6901 cgtctcgccc ctttctgttt ttatggcctt cggattttcg ggcctggtcg gaatcttttt
     6961 tgggttctat cctgcctaca aggcgtcaaa cctcaatccc attgatgcat tgagattcga
     7021 ataacctgct tctttgctcg gcatgtcaga gggccaatct tgaaacttca caaattcgaa
     7081 agaggtatcc ggctcgaaat agcaagactc agagttatgt acttgtccgc aggagcggac
     7141 cttgacaata aataagaaca gtgtgttgtt aggtgtgcag acccctatac agtcaaggag
     7201 ggctattgtc ttgcctagag aggcggatgc ctcttccgaa taatggaaag tcatgaatac
     7261 aatgggctaa attgattact tcttttatcg ttagcaaact ctgtctatta tccggtcttg
     7321 gcttacggcc agttgctgaa accgtgcttc attttgtaga ggaaatatcg ttcgaaccct
     7381 atcttcatcc acttgtacat gattccttcc ctgagatccg aatcctgtct tgggggaact
     7441 acagggctcg ccttcatata gaccgcggtg cctcccatgt ccataaggca gaggacttcc
     7501 attggaattt cgtcgggtgg cacgcttcct gtaatgtctg atacgattgc gagtgaagcg
     7561 gcctttgcca tcttgaccgt catatacccc gtcttgggca cacctgtagg caccggcgtc
     7621 tgttccgggg gagcaatagc aaccgcgact cccacagaga agatattctt gtattttgta
     7681 tgtctgtagt tcccgtcaac aggaataaag cccctcgggt tgccaagagg ggcaacggca
     7741 gcc
//
LOCUS       DATKOU010000263         7813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_375338, whole genome shotgun sequence.
ACCESSION   DATKOU010000263 DATKOU010000000
VERSION     DATKOU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375338"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VMU21_12525"
     CDS             complement(<1..615)
                     /locus_tag="VMU21_12525"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HUO78389.1"
                     /translation="MCDGHTDKNLSNRRREVRAVSNALPASAFVRTAPFATFSINSAF
                     VITFPSLKDLSTTTCLKLQNSFVCYLPLVSDKTIDLHLKTLEEALASDEMDITEIDDE
                     GSIPKLKFANRGAGTILILQGSIIKANKQDRAVKTTFVVHPHAVVEGDVFCIEASRWH
                     SVRGQKGYKPDRNLSADICMDFSKSKSSQQDIWRKIEEKRRRMQV"
     gene            complement(619..1065)
                     /locus_tag="VMU21_12530"
     CDS             complement(619..1065)
                     /locus_tag="VMU21_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78390.1"
                     /translation="MGKISQWQPKVCGGACFATLLLTQKVSYSKWNKWLMQNKDKYYP
                     LVEADASKRSSYERCLARWLVAEEIPYVFEPWVFKPDTGVYYLPDFYIMRVPCFLETK
                     GVWESGAFPTAKAFGEFTPIPYYVLTLPFLKHIKAQPKKEDKLSHV"
     gene            1640..2758
                     /locus_tag="VMU21_12535"
     CDS             1640..2758
                     /locus_tag="VMU21_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HUO78391.1"
                     /translation="MAFFNYVTKEITLKVVYYGPGLSGKTTNLQYLYSVLDPSSRGKF
                     ISLATESDRTLFFDFLPVELGKIKDFSVRFQLYTVPGQIRYNATRKLVLKGADAVVFV
                     ADSQTDLKEQNIESFANMRENLLSNNVNPDEIPVVIQYNKRDLSNVLSTEELRSDLNK
                     KDYPEIEAVAVDGKGVEETFRLITKILLKDIIKKHKVDIALPKESRISVPVPEKTVSE
                     KGLGAKDAISLRIPDKTTVQELPHLKDPISLKVPEKSPDQEVSQVKDTISLKIPEKEV
                     FRIDDLSIPSTTLKQREKEPPESNASPHEAFSSMTTTMLPEINRSLVALRETLTTLSA
                     EIRESGKRQAEMVRLLEEISTSMAQNKRKRRWFNSISL"
     gene            2776..3540
                     /locus_tag="VMU21_12540"
     CDS             2776..3540
                     /locus_tag="VMU21_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUO78392.1"
                     /translation="MNIKELISNLQKFYLLSLQGLLGTVRKPFYLRDTIEQMDYAGAG
                     SFFIIILVSLFIGMALSLQLAAELSVLGLKMYTGKVVGISIIREIGPVAAALVFTGRV
                     GSGIASELGSMVLGHQVDTLRVFGVDPIKKLVTPRMLSAIVMLPALTIIGDAASLFGG
                     YYIAVFMSHQSGHVYWSSVRDVLIFENVFAGSVKPFIFGFLIASISSYMGLSTKGGAI
                     GLRSATTRAVVLSIIMIIVADFILTRILLYILGFSV"
     gene            3537..4274
                     /locus_tag="VMU21_12545"
     CDS             3537..4274
                     /locus_tag="VMU21_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUO78393.1"
                     /translation="MIVFDKVWLLYGTRDILKDLSFSIHDNERVAILGESGEGKTTIL
                     KLIMGLVRPDRGNIYVDGEDITSKTEKELRPVRMKFSIVFQEGALFDSLNVKENVAFC
                     LREYTTMSEEEIDEKVRGLLKTVGVGHAMELMPEELSGGMHRRVAIARSLAGSEPHMF
                     LYDEPTSGLDPINAENICNLIRDLSKNGTGFLIVTHKIHDALKVANRFMFLKNGMIIF
                     DGDSDALLNSPIPEVRIFIKELGVHGT"
     gene            4307..5368
                     /locus_tag="VMU21_12550"
     CDS             4307..5368
                     /locus_tag="VMU21_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUO78394.1"
                     /translation="MFDMKKQLMWSKLKVGLVVTLALLVLLFTVFLAGSIEDIFSPKI
                     ALRAQIEDVRGLRKGAPVWISGIEVGSVKNIRLSGEYGTIVTFSVNRSTLKYIKKDSL
                     ASVLTMGLLGDKYVELSAGSPGAEPIQPGDMIKGASQIDLKNVMEVGTTSIQKMTDFL
                     TKLDHLVTKIEKGEGTISKFISDPSLYDNLKESTKALSSTLKEIRDARGTVRLLIDDP
                     TLYNRMVAASSSLEQFSKSVNESSGTLKKFIEDPALYNRMLTAASSVEEFSRKLNEGK
                     GTIKKLAEDAELYDNLNKASLQLSSVLERIDKGEGVAGSLLRDKELATELKESIVSLN
                     ELIKDIKEHPKKYFKFSVF"
     gene            complement(5434..6084)
                     /locus_tag="VMU21_12555"
     CDS             complement(5434..6084)
                     /locus_tag="VMU21_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HUO78395.1"
                     /translation="MDKERIDEALELLWHLEEEGNLELEHFRLSSDDPDLEELIGALK
                     SDDFITIEDSAVKLTVRGRLLSRGLIRRHRLAERLFTDVFELRGDQVETDACKIEHIL
                     SEELTESVCTFLGHPPTCPHGKPIPKGECCKKYKVEITPVVSRLSDFEVGSKGRIVFI
                     IPSETARLGRLSSIGIAPGSTVKLLQKMPSCVIQIDETTVAVDPDIAKEILVKKIV"
     gene            complement(6110..6721)
                     /locus_tag="VMU21_12560"
     CDS             complement(6110..6721)
                     /locus_tag="VMU21_12560"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HUO78396.1"
                     /translation="MDIYRYDESFRNEGFSRVAGIDEAGRGPLAGPVVASAVILPSMK
                     RIKGLRDSKKVPEKERKSLFQDLLCCAMDIGVGIVDAESIDRMNILRATRLAMEMAVR
                     DLSSRPDFLLIDAVELPSVDIRQISPFKGESKSASISAASIIAKVVRDGIMEDYDLMY
                     PEYGFAKHKGYSTREHVEKLLLHGPCEIHRKSFEKVMSLELPL"
     gene            complement(6725..7096)
                     /gene="rplS"
                     /locus_tag="VMU21_12565"
     CDS             complement(6725..7096)
                     /gene="rplS"
                     /locus_tag="VMU21_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HUO78397.1"
                     /translation="MNLMNAIEEGYKKEVPPFQVGDTVKVFVKVVEGDKERIQPFEGI
                     VIARRGSTIRETFMVRKVSYGIGVERIFPVHSPAIDRIEVLKRGSVRRAKLYYLRAKK
                     GKAAKIKDREKERLAVGQTTE"
     gene            complement(7093..>7813)
                     /gene="trmD"
                     /locus_tag="VMU21_12570"
     CDS             complement(7093..>7813)
                     /gene="trmD"
                     /locus_tag="VMU21_12570"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HUO78398.1"
                     /translation="TIFPSIFDAYLSESILKRAVEKGIVEFKVHQLRDYTVDKHRTVD
                     DYPYGGGPGMVMKQEPFFRAVKSIKADGAETFALMMSPQGRPFDQEMALELSKEKRRL
                     LFLCGRYEAIDERVRESLVDREVSIGDYVLTGGELPALVIIDSIVRLLPGALGDEYSA
                     KEESFSWGILDYPHYTRPPEFEGMKVPDVLLSGNHKEILLWRRKEALRRTLLRRPELI
                     QRTLLTDEDYRLLSEIKEEER"
BASE COUNT         2050 a         1967 c         1761 g         2035 t
ORIGIN      
        1 aacctgcatt ctcctgcgtt tctcctctat tttcctccaa atatcctgtt ggctcgattt
       61 ggattttgaa aaatccatgc atatatcggc actcaagtta cgatcaggct tatatccttt
      121 ttgtccccgt acggagtgcc atcttgaagc ctcaatacaa aagacatcac cctctaccac
      181 tgcatgagga tgcacaacaa aggtggtctt tacagccctg tcctgtttgt ttgccttgat
      241 gatgctcccc tgaaggatca ggatagttcc agccccacgg ttggcgaatt tcagcttcgg
      301 gatacttccc tcatcgtcaa tttccgtgat gtccatttca tcagaggcaa gggcttcctc
      361 caatgtcttg aggtgtaagt ctattgtttt atccgacacc aaaggcagat aacagacaaa
      421 agagttttga agctttaggc acgtagttgt agataaatct ttaagggagg ggaaggttat
      481 gacaaaagcc gaattaatcg agaaggtagc aaagggggca gttcttacaa aggcggatgc
      541 gggcagggca ttggataccg ccctaacctc acgtcttcgg ttcgataagt ttttgtcagt
      601 gtgtccatcg catatttgct atacatgact tagcttgtcc tccttcttcg gttgcgcttt
      661 gatgtgcttg agaaatggca atgtcagcac ataataggga attggtgtga actcaccgaa
      721 cgcctttgct gtcgggaacg ccccagattc ccacacgcct ttcgtttcta ggaaacacgg
      781 aacacgcatt atatagaagt ctggaagata ataaacacca gtgtcgggtt taaaaaccca
      841 cggctcaaac acataaggta tctcctctgc tactaaccat cgggcgagac aacgctcata
      901 ggaactccgc ttgcttgcat ctgcctcgac aaggggataa tacttatcct tattctgcat
      961 aagccacttg ttccatttgg aataggaaac cttttgagtg aggagcaatg tggcaaagca
     1021 agcacctccg cacactttcg gctgccactg ggaaatcttc cccatttcat aggtgttctt
     1081 aaaagtatct ccgcacactt cgcatttgct gatcacctga tttctccttt taaacgtttt
     1141 tgactaatct gtttcataaa gaagttacaa gaatatctta tatcaattct acctcaaaaa
     1201 gttttaacgt cctattcggg taatggctct cacgacccat ccttctcaaa atcccctcag
     1261 aacaaaacat cccacagggg aaaggggaat gtcctgccag gtgtctagaa aaggtcgcgg
     1321 tgggttgtgg ggctggttat tgaggctgtt gggttagtct tccgtgatgc agaaggctgc
     1381 ggggatttga ggggtaaatt acctctcaat cattagcctg aacaccgttt cttctacctg
     1441 aatatttagt atagattttc tccttttttt ctaccatttt agaatttggt agaagaaaac
     1501 aggattaaat gaagaatttc gtcataccat gtaagttacg taacttcgtg acaattataa
     1561 atttatattc gtatatgcag atagaactaa atgttaagga actggtttcg gaaaaacggt
     1621 gtttatgcta taatttagag tggctttttt caattacgta acaaaagaaa ttaccttaaa
     1681 ggtcgtatat tacggtcccg gtctgagcgg aaagaccaca aatctccagt atctctattc
     1741 cgtcctcgac ccttcatcca ggggtaaatt tatctccctt gccacagagt ctgacaggac
     1801 tctgtttttt gattttctcc ccgttgaatt aggaaagata aaggacttct cggtgaggtt
     1861 ccagctttac accgttcccg gacagatacg atataatgcc acgagaaaac tcgtattgaa
     1921 gggtgccgat gctgtggtat tcgtggccga ttcccaaacg gatttgaagg agcagaatat
     1981 tgagagtttt gcgaatatgc gggaaaatct cctctccaac aatgtgaacc ctgatgagat
     2041 ccccgtggtg attcagtata acaaacgtga tctttccaat gtcctttcca cagaagaact
     2101 cagaagtgac ctgaacaaaa aggactatcc ggaaatcgaa gcggttgccg ttgacggcaa
     2161 gggggttgag gagacattcc gtctcatcac aaaaatactc ctgaaagata tcattaagaa
     2221 gcacaaggtg gacattgccc tccccaaaga gtcgcgtatc tcggtacctg ttcctgaaaa
     2281 aactgtttca gaaaagggtc ttggggcaaa ggatgccata tcgctgagga ttccggacaa
     2341 aacaacagtt caggaactcc cccacctaaa ggaccccata tccctgaagg ttcctgaaaa
     2401 atcgccggac caggaagtgt cccaagttaa ggacaccata tccctgaaga ttcccgagaa
     2461 agaggtattt agaattgatg atttaagcat accgtcaacg accttgaaac agagagaaaa
     2521 ggaaccccct gaaagtaacg catctcccca cgaagcgttc agttccatga caacaaccat
     2581 gctccctgaa attaaccggt cacttgttgc cctgcgagaa accctgacca ctctctccgc
     2641 agagataaga gaatcaggaa aacgacaggc agaaatggta agattgcttg aggaaatcag
     2701 cacctccatg gcccagaaca agaggaaaag acggtggttt aactccattt ctctttaaga
     2761 acctccccac gcataatgaa cataaaggaa ctgataagca acctccagaa gttttacctc
     2821 ctctctctcc aagggctcct gggaaccgtg aggaagccat tctacctgag agacaccatt
     2881 gaacagatgg actacgcagg agccggatcc ttctttatca ttattcttgt atcccttttt
     2941 atagggatgg ccctttccct gcagctggct gcagaacttt ccgtcctcgg cctcaagatg
     3001 tacacaggaa aggtcgtggg catttccatc atcagggaga taggtcctgt agccgctgcc
     3061 ctcgtgttta ccggaagagt tggctcggga attgcctctg aactcggttc aatggtgctc
     3121 ggccaccagg tcgatactct aagggtcttt ggcgtggacc ctataaaaaa gcttgtcacc
     3181 ccccgcatgc tgagtgccat tgtgatgctg cctgccctca ctattatcgg cgatgccgcc
     3241 tccctctttg gaggatatta catcgcggtc tttatgagcc atcagagcgg tcatgtatac
     3301 tggagctctg tccgagacgt cctcatcttt gaaaatgtct ttgcaggttc ggtgaaaccg
     3361 ttcatcttcg ggttcctcat tgcgtccatc agcagttata tgggtctttc cacaaagggc
     3421 ggggccatcg gtctcagaag cgcgaccacc cgtgcggtgg tgctctccat tatcatgatc
     3481 atcgttgctg actttatcct gacaaggata ttgctctaca tcctggggtt ctccgtatga
     3541 tcgtatttga caaggtgtgg ctcttatacg gaacgcggga catcctgaag gatttgagct
     3601 tttccattca cgataacgaa cgggtggcaa ttcttgggga gagcggagaa ggaaaaacca
     3661 ccatcctcaa gcttatcatg gggcttgtaa gacctgatcg cgggaacata tacgtcgatg
     3721 gagaagatat cacttctaag acggaaaaag aactcaggcc ggtgagaatg aaattcagca
     3781 tcgtcttcca ggaaggtgcc ctctttgatt ccctcaatgt caaggagaat gttgcatttt
     3841 gtttgaggga atacactacc atgtcagagg aggagattga cgaaaaggtg aggggattgc
     3901 tgaaaacggt aggggtagga catgccatgg agctcatgcc cgaagaattg agcggaggga
     3961 tgcaccggag ggttgccatt gcacgatccc ttgcgggttc agaaccgcat atgtttctct
     4021 acgacgaacc cacgtcaggc cttgatccta taaatgcgga aaacatttgc aaccttatcc
     4081 gcgatctctc aaagaacggc acagggtttc tcatcgttac ccacaagatt catgacgcat
     4141 taaaggttgc aaacaggttt atgtttctca aaaatggtat gataatattc gatggcgaca
     4201 gtgatgcgct cctcaattcc cctataccgg aggttagaat attcatcaag gaactgggcg
     4261 tccatggtac gtgaacggtc agtactgtcc ttgaaggatg gcgcatatgt ttgatatgaa
     4321 aaaacagctc atgtggtcaa aactgaaggt cggtctggtg gtgacccttg ccctcctcgt
     4381 cctcttgttc accgtttttc tcgcaggcag tattgaagac atcttctctc ctaaaatagc
     4441 attgcgcgca cagatagagg atgtgagggg tttgcggaaa ggtgctccgg tctggatatc
     4501 agggattgag gtggggtcgg taaagaatat caggctgagc ggagagtacg gaacaatcgt
     4561 cactttttcc gtgaatagaa gcaccctaaa atatatcaag aaggattccc ttgcaagcgt
     4621 cctcaccatg ggactgctgg gcgataaata tgtggagctg tccgcaggtt cacccggggc
     4681 tgaacccata cagcccggag acatgataaa gggcgcatca cagattgatc tgaagaatgt
     4741 gatggaggtc ggtaccacat ccatacagaa gatgactgac ttcttaacaa aactggacca
     4801 tcttgtcaca aagatagaaa agggagaagg gaccatttct aaatttatca gcgacccttc
     4861 gctttatgac aatctcaagg agtccacaaa ggcattgtcg tcgaccctga aagagataag
     4921 ggatgcgcgg gggacagtaa gattgctgat tgatgaccct acgctctata acagaatggt
     4981 ggctgcttca tcctctcttg agcaattcag caaatccgtc aatgagagtt cgggaacatt
     5041 gaagaagttc atagaagacc ccgcgctcta taacagaatg ctcactgctg cctcctctgt
     5101 ggaagagttc agcaggaagc tcaatgaagg aaaagggacg atcaagaaac tagctgaaga
     5161 cgcagaactg tatgacaacc tgaataaggc atcactgcaa ctctcctccg tgctggagcg
     5221 catagacaag ggagaagggg tagcggggag tcttttgaga gacaaggaac ttgccaccga
     5281 actaaaggaa agcattgtct ctctgaacga actgattaag gacataaagg aacatccgaa
     5341 gaaatatttc aaattcagcg tattctgagt ctcccgtatg ccaggtaccg gaacagctgc
     5401 ccgtcccatg atggcatctt ggccgagtta cccttaaacg atctttttta ccagaatttc
     5461 ctttgcgata tccgggtcta cagcaacagt ggtctcatcg atctgaatga cacaggaggg
     5521 cattttctgt aaaagcttga cggtactgcc aggggctatc cctatagaac tgagccttcc
     5581 gagtcttgcg gtttcggacg gaatgatgaa tactattctc ccctttgagc ccacctcaaa
     5641 atccgaaagc cgcgaaacca ccggagtgat ctccactttg tactttttgc agcactcacc
     5701 ttttggtatg ggcttgccgt gggggcatgt ggggggatga cccaaaaatg tgcagacgct
     5761 ctcagttaat tcctcactca gtatgtgttc tatcttgcat gcatccgtct caacctggtc
     5821 cccgcgcaat tcaaacacat cggtgaaaag tcgctctgca agacggtggc gtcgtataag
     5881 tccccgggaa agaagacgtc ccctaacagt gagtttgact gcagaatctt ctatagtgat
     5941 gaagtcatca ctcttgagtg caccgatcag ttcttcgagg tcaggatcgt cagaactcaa
     6001 tctgaagtgt tcaagctcaa gatttccctc ttcctcaagg tgccaaagga gttcaagcgc
     6061 ctcatctatc ctttctttat ccatccgccc tcttgacact cttcagccct cacaggggaa
     6121 gctccagaga catgaccttc tcgaaactct ttctgtggat ctcgcacggc ccgtggagca
     6181 ggagcttctc gacatgttcc cttgtggaat accccttgtg ttttgcaaaa ccgtattcag
     6241 ggtacataag gtcataatcc tccataatac cgtcccttac caccttggca ataatggaag
     6301 cagcagatat ggacgcactt tttgattcac ccttgaaagg ggaaatctgc ctgatgtcta
     6361 ctgagggcag ttcaacggca tcgataagga gaaaatcagg tcttgaggag aggtctctca
     6421 ctgccatctc catggcaagc cttgttgccc tgagtatgtt cattctgtca atgctctccg
     6481 catccacgat ccccaccccg atgtccattg cgcaacacaa caggtcctga aagagtgatt
     6541 tcctctcttt ctcgggaact ttttttgagt ccctcaaccc tttgatcctc ttcatggaag
     6601 ggaggataac cgctgaagca accacaggac cggcgagggg gcccctcccc gcctcatcga
     6661 tacccgcgac acgggagaac ccttcatttc tgaaggattc atcataccgg tagatgtcca
     6721 ttgactattc tgtcgtctgt cccaccgcca gcctctcttt ttccctatcc ttgatctttg
     6781 ccgccttccc cttcttcgcc ctcagataat agagtttcgc ccttctcacc gaaccccttt
     6841 taagcacctc tatcctgtca atagcagggg aatgcacggg gaatatcctc tccaccccga
     6901 ttccataaga cacctttctc accatgaacg tttccctgat agtgctgcca cgtcttgcga
     6961 tcacaatacc ttcgaagggc tgaatcctct ccttgtctcc ctcaaccacc ttcacaaaga
     7021 ccttcaccgt atcccccacc tgaaaggggg gcacttcttt cttgtatccc tcttcaattg
     7081 cattcatcag gttcatcttt cttcctcctt aatttccgac agtaatcggt aatcttcatc
     7141 agtgagcagc gtccgctgta taagttcagg tctcctgaga agtgtcctcc ttaacgcctc
     7201 tttccttctc cacaggagta tctctttgtg atttccggaa agcaatacat cgggcacctt
     7261 catcccctca aattcaggag gtcttgtgta atgggggtaa tccagtatcc cccacgaaaa
     7321 cgattcctcc ttcgcagaat attcgtcacc cagcgctccg ggcaaaagcc tcacaatgct
     7381 gtctattata accaaagcag gcaattctcc gccagtcaaa acataatcac caatggacac
     7441 ctcccgatca accagtgact cccgcacacg ttcatcaatg gcctcgtatc ttccgcagag
     7501 aaacaaaagc ctcctctttt ccttcgataa ttcaagggcc atctcctgat caaaaggcct
     7561 cccctggggg ctcatcataa gggcaaaggt ctcggcccca tccgccttta tggatttcac
     7621 cgccctgaag aaaggctcct gcttcatcac cataccgggc cctcccccgt acggataatc
     7681 atccaccgtc ctgtgcttat caacggtata atctctgagc tggtggacct tgaactcaac
     7741 aattcctttc tccacggccc tcttcaggat actttcggag agatacgcat caaatatact
     7801 ggggaatatg gta
//
LOCUS       DATKOU010000264         3373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_5386169, whole genome shotgun sequence.
ACCESSION   DATKOU010000264 DATKOU010000000
VERSION     DATKOU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5386169"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1191)
                     /locus_tag="VMU21_12575"
     CDS             complement(join(62..930,930..1191))
                     /locus_tag="VMU21_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611730.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HUO78399.1"
                     /translation="MSKRPRRNHSAAFKAKVAVEALKDGQSLVELAERFQVHPNQITE
                     WKKQLLERAEEVFSKGKKSESGPDLKELHAKIGQLAMENDFLSGRARAHSRAERKEMI
                     DRGHPLSLTRQSEVLELSRSSLYYEAVPISARDLELMRLIDELHLKHPFMGSRSIRDQ
                     LQDMGHQVGREHVSTLMNKMGIAALYRKPRLSKPHPDHKVYSYLLRGLEISRANHVWA
                     TDITYIPMARGFCYLVAIMDWASRKVMAWRLSNTLDASFCTEALEEAIQKYGTPDIFN
                     TDRGSQFTSDAFIGILENHGIDISMDGKGRWLDNVFVERLWRSVKYEDVYLKGYSSIA
                     EARQGLKEYFEFYNHRRRHQSLDRRTPDDVYWSTLPQMQVAA"
     gene            complement(1418..1591)
                     /locus_tag="VMU21_12580"
     CDS             complement(1418..1591)
                     /locus_tag="VMU21_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78400.1"
                     /translation="MKLFLALVMILFAVGCASKDYVRQQLEPLQDKINKLQTCCEQNK
                     KAMKKSFELQQKK"
     gene            1770..2885
                     /locus_tag="VMU21_12585"
     CDS             1770..2885
                     /locus_tag="VMU21_12585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUO78401.1"
                     /translation="MDTPLHYGSWKIATIMGIPIRVHFSWLLIFGLITWSLSTLYFPK
                     AAPQLPELSYWVGGSIAALLLFVSVAFHELSHSLVALRYRLPIVNITLFIFGGVSQMK
                     GEPPNPKAELMIAIAGPFSSFILAVFFSLINSLVTYPVAKALFSYLVQLNIVLGVFNL
                     IPGFPMDGGRVVRASLWRKTNDFFYATRRASRYGQNIALFFIIFGLLSLFAGFPGALW
                     LMIIGWFLHTAAHATYQQANLQETLSGIKVRDIMVRDVVIIPSGESVEHVVNEYFLRY
                     GYGGFPVMDVANFIGFITLKDIKNIPKEQWRDTSISKIVVPHQKRWEVSVDDDALKAL
                     EVMISEDKGRLVVKEKERVVGLITRNGITSYIQIMGR"
     gene            2974..>3373
                     /locus_tag="VMU21_12590"
     CDS             2974..>3373
                     /locus_tag="VMU21_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUO78402.1"
                     /translation="MEIKRVLEESSHYIMNTYERFPVVLRKGRGITVWGSDGKEYIDF
                     LGGIAVNVLGHCHPRVVIALQKQAQRLLHVSNLYHNEPQIKLAKLLVEHSFADKVFFC
                     NSGAEANEAAIKLARKYAKENMPGNRFEIIT"
BASE COUNT          787 a          844 c          804 g          938 t
ORIGIN      
        1 agaggtggcc gggtttgttt ggacagctga gagcagtttt taagtgataa gctgttctgc
       61 ctcatgccgc tacctgcatc tgaggtagtg tactccagta aacatcatcc ggggtcctgc
      121 ggtcaaggct ttgatgccga cgcctgtgat tgtagaattc aaagtattct ttgagtccct
      181 gccgagcttc ggcaatcgag ctgtagcctt tcaaatatac gtcttcatat ttcacactcc
      241 gccagaggcg ttcgacgaac acattgtcca gccagcggcc cttgccgtcc atgctgatgt
      301 cgataccatg attttcgagg attccaataa atgcatccga ggtgaactgg ctgccccggt
      361 cagtgttgaa gatatccggc gtcccatact tttgtatagc ttcttcaagc gcctctgtgc
      421 agaaggaggc atccagcgtg ttggacagcc gccatgccat gaccttccgg ctggcccagt
      481 ccataatagc cacgaggtaa cagaatcccc tcgccatcgg gatatacgtt atgtcagtcg
      541 cccaaacgtg attggccctg ctgatctcca agcctctaag cagatacgaa tagaccttgt
      601 ggtcaggatg cggcttcgac aacctgggct tacggtacag ggcagctatg cccatcttat
      661 tcatcaacgt gctgacatgc tcgcggccga cctggtgccc catatcctga agctggtccc
      721 ggatgctcct gcttcccata aacgggtgtt tcagatgaag ctcgtcgata agtctcatca
      781 attcaagatc acgggcgctg atcggtacag cctcgtagta aagactcgat cgagacaact
      841 caaggacctc gctttggcgg gtcaacgaca atggatgtcc ccggtcgatc atttctttgc
      901 gctcggctcg gctatgcgcc cgagcgcgac cgataaaaaa tcgttctcca ttgccaactg
      961 tccgatcttg gcgtgcaact ccttgaggtc ggggcctgat tcggatttct tccccttgga
     1021 gaatacctct tctgcgcgct ccagcagctg ttttttccac tccgtgatct ggttgggatg
     1081 cacctggaat ctctcggcca actctacaag gctctgtccg tctttaagcg cttccacggc
     1141 aacctttgct ttgaatgctg cgctgtggtt ccttctgggt cttttactca ttctgctgct
     1201 cctttcggtc tctgacaggg ccatcagcca atttcggctt cgccatttgc cctgtatggg
     1261 gcgatctcga catgactcaa tgtctgatac cccctgtttc tactttagag cagctttacc
     1321 acttatgtca ctgtccagtt tttcccggcc agctctgcat aactgcaaag gagcccaaat
     1381 tgtgtaaact atttaggccc ctttccctaa ggtggtccta tttcttttgc tgtaattcaa
     1441 aagacttctt catagccttt ttgttctgct cgcaacaggt ttgcaatttg ttgattttat
     1501 cctgcagagg ctctaactgc tgccgcacat aatcctttga agcacatcct actgcaaata
     1561 atatcataac gagcgcaaga aaaagtttca taatctcacc tccttcatca agtgtgaagt
     1621 ttatgaaata ataatacacc aagaaaatac agctttcaag cataaagcac atcgtatcat
     1681 ctgaggggtc tcgatctcca atgtcccctg ttcacatatt atggtgacga tggtagtata
     1741 tctctagggc gtcttctcca gggtgactta tggacacacc gttacattac ggttcatgga
     1801 aaatcgcaac aattatgggt atccccataa gggttcattt ttcgtggctt cttatctttg
     1861 gcctcatcac gtggtcacta tccactctct attttcctaa ggctgcaccc caactgccgg
     1921 aactttccta ttgggtgggt ggctctattg ctgccttact cctgtttgta tctgtggcgt
     1981 tccatgagct ctcccattcc cttgtggcat tgaggtacag actcccaata gtgaatatca
     2041 cgctttttat attcggcgga gtatcgcaga tgaagggaga acctccgaac ccaaaggctg
     2101 agctcatgat agccattgca ggaccgtttt ccagtttcat cctggctgta ttcttttctc
     2161 tcatcaacag ccttgtaaca tatcctgttg ccaaggcact tttttcgtat ctcgtacagc
     2221 tgaatattgt gctcggcgtg ttcaatctca tccccggatt tcccatggat gggggcaggg
     2281 tggtgagggc atcgctctgg agaaagacaa acgacttttt ttacgccaca agaagggcct
     2341 ccagatatgg tcagaatatt gcgctctttt tcatcatctt tggtctcttg tccctgtttg
     2401 ccggattccc gggtgccctg tggctcatga ttataggctg gtttctccac actgccgcgc
     2461 acgcgactta tcaacaggca aaccttcagg agaccctcag cggtataaag gtgagggaca
     2521 ttatggtgag agatgtggtg atcataccct ccggtgaaag cgtggagcat gtggtgaatg
     2581 aatatttcct gagatacggc tacggcggat ttccggtgat ggacgtggca aatttcatcg
     2641 ggtttataac tctaaaggat atcaagaata tacccaagga acaatggcgc gacacaagta
     2701 tatccaagat cgtggtgccc catcagaaga gatgggaggt ctccgtggat gatgacgcac
     2761 taaaggcctt agaggttatg atcagcgagg ataaaggcag attggtagta aaggaaaagg
     2821 aaagggtagt cggtttaatt acgaggaacg gaatcacaag ttatatccag ataatgggga
     2881 gataacggtc ctcttattga agtaagacgg ttctcctcca cgaaacccct gttattattt
     2941 gatttttctt gtctcgttcc gttattattg actatggaaa tcaagagagt tctcgaggaa
     3001 tcaagccact atatcatgaa cacctatgaa cggtttcccg ttgttctgag aaagggcagg
     3061 gggataactg tctgggggtc ggacggaaag gaatatatcg actttttggg tggcattgcg
     3121 gtgaacgtcc tcggccactg ccatccccgg gtcgtgatcg cgctccagaa acaggcccag
     3181 aggctcctcc atgtctctaa tctctatcat aacgaaccgc aaataaaact cgcaaagctc
     3241 ctggtggaac attctttcgc ggacaaggtc tttttttgca attcaggggc agaggcgaat
     3301 gaggcggcca taaagcttgc gaggaaatat gcaaaagaaa atatgccggg aaaccgtttt
     3361 gagattatca ctg
//
LOCUS       DATKOU010000265         8244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2292836, whole genome shotgun sequence.
ACCESSION   DATKOU010000265 DATKOU010000000
VERSION     DATKOU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292836"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VMU21_12595"
     CDS             <1..245
                     /locus_tag="VMU21_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HUO78403.1"
                     /translation="QKVVLVDDVLFTGRSIRAALDALMDIGRPANIQLAVLIDRGHRE
                     LPIKADFVGKNIPTAVNENVEVSLEEEGKEDRVTIE"
     gene            514..1437
                     /locus_tag="VMU21_12600"
     CDS             514..1437
                     /locus_tag="VMU21_12600"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HUO78404.1"
                     /translation="MKLSSKDLLGIKDLSKDEIILVLDTASGFKDVLRRDIKKVPALR
                     GKTAVNLFFEPSTRTKTSFELAEKRLSLDVVNFAVTTSSVVKGESIIDTALNVQALGA
                     EFMVIRHPSSGVPHLVSKHIHASVINAGDGINEHPTQALLDAFTMKEKMGRLEGLVVA
                     IVGDVLHSRVAKSDCYLLTKFGAEVRLIGPPTLVPLEMESVGAKVFHDMEEGLRDADV
                     VMMLRIQMERQDRGFFPSTLEYARYWGLTTERLSLAKKEAIVMHPGPMNRGVEIVSEI
                     ADSPRSVILEQVTNGLAVRMAVLYLLAGIKE"
     gene            1434..2732
                     /locus_tag="VMU21_12605"
     CDS             1434..2732
                     /locus_tag="VMU21_12605"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HUO78405.1"
                     /translation="MNLLIKNGHIIDPSQGIDGVGDLVIENNRIKEISLERKGKNSAE
                     GSASGLQGFRTIDASGLCVLPGLIDMHTHLREPGFEYKETIKTGTMAAVKGGFSSVCA
                     MPNTNPVNDNSSVTDFIIRKAIQEGACTVYPIGAITKGQKGEELAEMGIIYAEGCVAF
                     SDDGRPVMSSLVMRRALEYSKAFNVPIISHCEDLDLSEGGVMNEGLLSVTLGLRGVPP
                     EAEEVMVARDIALAGLTKGRLHIAHVSTAGSVRLIREAKARGILISAETCPHYFSITE
                     KAVEGYNTNAKVNPPLRGQEDVDAIKKGLKDGTIDVIATDHAPHHRDEKLREFDHAPS
                     GISGLETAMGLSLRLFEQGVLTLSQLVEKMAKNPAVILGIDKGTLKPGADADITILDL
                     NKEFRVDPGEFVSKGKNTPFEGWVLKGIPVITICKGKVHE"
     gene            2725..3843
                     /gene="carA"
                     /locus_tag="VMU21_12610"
     CDS             2725..3843
                     /gene="carA"
                     /locus_tag="VMU21_12610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HUO78406.1"
                     /translation="MNKAMLVLSDGTAFEGVSFGAEGETLGEVVFNTSMTGYQEILTD
                     PSYKGQIVTMTYTQIGNYGVNEEDTESRGGIKAEGFIVKEACRFPSNWRSSMSLIEYL
                     KKYDIVGIQGIDTRGLTRHLRNHGAQMGIISTTDLRPENLLKKVRAHPGISAFDLVKD
                     VSTPEVYSWTEGCWKWCAVQKGETAHKVVVYDFGVKFNILRNLAEAGFGVTVVPAQTP
                     AEKVLEMNPDGIILSNGPGDPQTVTYAIENAKKLMGKKPIFGICLGHQILGLAMGGRT
                     YKLKFGHHGGNHPVKDISTGRVEITSQNHNYCVDIVSVGGKARLTHKNLYDGTEEGME
                     HVELPVFSVQHHPEAGPGPNDSVHLFRRFREMIERSTC"
     gene            3837..4361
                     /locus_tag="VMU21_12615"
     CDS             3837..4361
                     /locus_tag="VMU21_12615"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HUO78407.1"
                     /translation="MLKEITSDIYHLLHPKMAFFLTSADKKGNPNVMTCAWATPVSEE
                     PPLVVVCVAKESYTAELIKQTKEFVINIPTKKLLKALWVCGKTSGRDVDKFKKARLKG
                     IGAIRVKPFVVEGCIGYIECRLWKAVDAGECYAFFGKVLSAYGDDNYIEKGLWTKRAE
                     IPLHLGGSRIVYFK"
     gene            4369..4995
                     /locus_tag="VMU21_12620"
     CDS             4369..4995
                     /locus_tag="VMU21_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78408.1"
                     /translation="MSAEAYQIEKSENGVVVRTTSFRTERRSVLHSGIFNRELASSLA
                     AGAVMVMVGFFFAHHFRITVVHFIAVAFVFAVLFILFRIYAFREAVLETTFDWERKTI
                     TISLKRTMGSRHRSYPMDALAGIRIHHLSMKPENLDAVRLVEKVAVQHGTVIPGFGKV
                     EDFYSVELDFREKKIVIFSAKDQKGAESFVNDLRSSLAGFLPSVVWET"
     gene            4995..5273
                     /locus_tag="VMU21_12625"
     CDS             4995..5273
                     /locus_tag="VMU21_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78409.1"
                     /translation="MGAVYDELYISTMREMDVCAGAIRKLKRVIEDMETKYNLKTPEF
                     VERFSKGGMKDNKDFMEWRGSYERLKHWEGRLKEFKDILQNAKDESVR"
     gene            5276..>8244
                     /gene="carB"
                     /locus_tag="VMU21_12630"
     CDS             5276..>8244
                     /gene="carB"
                     /locus_tag="VMU21_12630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HUO78410.1"
                     /translation="MPKRTDLRKILLIGSGPIVIGQACEFDYSGTQACKALREEGYKV
                     VLVNSNPATIMTDPEMADAVYIEPLTPEIIELIIEKERPDALLPTMGGQTALNLAVEL
                     AESGVLGKYGVELIGAKLPAIKKAEDRELFKEAMEKIGLEVPRSAYIGSVKQGLDSIE
                     HIGFPAILRPSFTLGGTGGGIAYNMEEYMELLERGLKLSPVHQILVEESVIGWKEYEL
                     EVMRDMKDNVVIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQRMRDASIAIIREIG
                     VDTGGSNIQFALSPENGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGLSLDE
                     IPNDITKETPASFEPTIDYVVTKFPRFAFEKFPEADATLTTQMKSVGEAMSIGRTFSE
                     SLQKALRSLEIGSYGFEELQVSASELKARLKIPNAERIWYVTQALRNGMPVNEIYKLT
                     NIDPWFLHHITQILELENEIKSYARGIKEEKTKSLAPYLLPKDLLTEAKVKGFSDRTI
                     AGLLKVPEKDIRHLRKQWNLHAVYKMVDTCAAEFEAYTPYLYSTYEKPFSRIAHVAGR
                     KEEEESGSMPYASSVENESHPTHKRKVIILGSGPNRIGQGIEFDYCCVHAVFALKELG
                     YETIMVNCNPETVSTDYDTSDRLYFEPLTIEDVLSIIEVEKPDGVIVQFGGQTPLKLA
                     VPLEKEGVKILGTSPDSIDRAEDRKRFKELLHTLNLRQAESDTAMSPEEALEIAGKIG
                     YPVMVRPSYVLGGRAMEIVFDENSLADYMRRAVKASPEHPVLVDKYLEDAIEVDVDAL
                     SDGIDVIVGGVMEHIEEAGIHSGDSACSLPPYSLSNSIVDEIKRQTKALANELKVIGL
                     MNIQFAVKEGEIYILEVNPRASRTVPFVSKATGVPLAKMAAKIMGGMTLKELGLTSEK
                     EITHIAVKEAVFPFDRFHGVDTILGPEMKSTGEVMGIDEDFGLAYAKAEESSHNKLPL
                     SGNIFISVKDKDKPGTC"
BASE COUNT         2208 a         1822 c         2404 g         1810 t
ORIGIN      
        1 accagaaggt ggtactcgtg gatgatgtcc ttttcaccgg gaggtctata agggctgcgc
       61 tggatgccct tatggatatc ggcaggccgg caaacataca actcgcggta ctgatcgaca
      121 gggggcaccg ggagcttccc ataaaggctg attttgtggg aaagaatatc cccactgccg
      181 ttaatgaaaa tgtggaggtg agccttgaag aggaaggaaa ggaagacagg gtgaccatag
      241 aatgagcccc gttagacctc tttggcatta tacttatgtt ctcaaaagtg aaaccagtgg
      301 aaaactttac gctggtgcta caaataattt agagcagcgg ctgaagcaac ataataatgg
      361 aatggttttt tcaaccaaat atatgagacc attacagttg atttatttta aggcttgctt
      421 aaacaaggac gatgcttatc gtagagagat atatttaaag tcaggaatgg gaaagcgcta
      481 tttcaggaac cgcctcagag gaggtctaac ggggtgaagc tctcttcaaa ggacctgctg
      541 ggaatcaagg acctctcaaa ggatgagatc atccttgtcc tggatacagc ctcagggttt
      601 aaggatgtgc tcaggaggga tattaagaag gtcccggccc tgaggggaaa aactgcggtg
      661 aacctctttt ttgagccctc aacccggaca aaaacctcct ttgagctggc tgaaaaacgg
      721 cttagcctgg acgtggtgaa cttcgcagtg accacaagca gcgtggtgaa aggggagagc
      781 attatcgata ctgcccttaa tgtgcaggcc ctgggcgctg agtttatggt catacgccac
      841 ccatcatcag gggtgccgca tctcgtttca aagcatatcc atgcctctgt gatcaatgcg
      901 ggtgacggga tcaacgagca tccgacccag gcgctccttg atgcttttac catgaaggaa
      961 aagatgggga ggcttgaagg cctcgttgtg gcaatcgtgg gagatgttct tcatagcagg
     1021 gtggcgaaat ctgactgtta tctcctcaca aaattcggcg ctgaggtgag gctcattggt
     1081 cctcccaccc ttgtccctct ggagatggaa tcagtggggg ctaaggtctt ccatgatatg
     1141 gaagaaggcc tccgggatgc tgatgtggtg atgatgctca ggatccagat ggagaggcag
     1201 gacagggggt ttttcccttc aactctggag tacgccagat actggggact taccacagaa
     1261 aggctttccc tggcaaaaaa agaggctatt gtcatgcacc cgggacccat gaacagggga
     1321 gtggaaattg tgtcggagat tgcggacagt ccgaggtcag tgattcttga acaggtcacc
     1381 aacggcctcg cggtgcggat ggctgtttta tacctccttg cggggataaa ggaatgaatt
     1441 tacttattaa gaatggccat atcatagacc cgtcccaggg gattgacgga gtcggagatc
     1501 tggtcataga gaataacagg ataaaggaaa tcagtctgga gcgaaagggc aagaatagcg
     1561 cggaggggag tgcctccggg cttcagggct tccggaccat tgatgcctca gggctctgcg
     1621 tacttcccgg gctgatcgat atgcataccc acctgagaga gccgggattc gaatacaagg
     1681 agaccataaa aacagggact atggctgcag tgaagggtgg tttcagctcg gtctgcgcaa
     1741 tgcccaatac gaatccggtg aacgacaatt caagcgtgac ggattttatc atcagaaagg
     1801 cgatccagga aggggcatgc accgtctatc ccatcggtgc catcaccaag ggacagaagg
     1861 gtgaggagct tgcggagatg ggcattatat acgctgaagg gtgtgtggca ttttctgatg
     1921 acggaaggcc ggtgatgagc agcctggtca tgcggagggc actggagtat tccaaggcat
     1981 ttaatgtccc cattatttca cactgcgaag acctcgacct ctctgaggga ggggtgatga
     2041 atgagggtct cttatccgtg accctcgggc tccggggagt tcctccggag gccgaagagg
     2101 tgatggttgc cagagatatc gcccttgcag gcctcacaaa gggaaggctc catatcgccc
     2161 atgtttctac cgccggctct gtgagattga tcagggaagc caaagcacgg ggaatactga
     2221 ttagcgcgga gacatgtccc cattatttca gtattacgga aaaggcagtg gaggggtata
     2281 acaccaatgc aaaggtcaat cctcccttga ggggacagga ggatgtggat gcaataaaga
     2341 agggattaaa ggacggcacc attgatgtga ttgccactga ccatgccccg caccacaggg
     2401 acgaaaaact gcgggaattt gaccatgccc cttcgggcat atcagggctg gagaccgcaa
     2461 tggggctgag cctgagactt tttgagcaag gggtgctgac cctctcacag ctcgttgaaa
     2521 aaatggcgaa gaatcctgcc gtcattcttg gcatagacaa gggtacgctg aagcccggcg
     2581 ctgacgcgga tattacgatt ctggatctga ataaggaatt cagggttgat cccggggagt
     2641 tcgtttccaa agggaagaat acgccctttg aaggatgggt gctgaaaggc attccggtga
     2701 ttaccatatg caagggaaag gtacatgaat aaggcaatgc ttgtgctatc agacggaacg
     2761 gcctttgagg gtgtatcctt tggagcagag ggagaaaccc tgggtgaggt ggtctttaat
     2821 acctccatga ccggatatca ggaaatcctt accgatccct cctacaaggg acagatcgtc
     2881 actatgacct acactcaaat aggaaattac ggggtgaatg aagaagatac ggagtccaga
     2941 ggaggaataa aggcagaggg ttttatcgtg aaagaggcgt gtcgctttcc gtccaactgg
     3001 cggtcatcca tgagccttat cgaatattta aaaaaatatg acattgtggg tattcagggc
     3061 attgatacta ggggattgac aagacatttg aggaatcacg gcgcacagat gggaattatt
     3121 tccaccaccg atctccgacc agagaacctt ttgaaaaagg tgagggccca ccccggtata
     3181 tcggcctttg atctggtgaa ggacgtgagc actccggagg tctattcctg gacagaaggg
     3241 tgctggaagt ggtgcgcggt ccaaaagggt gagactgcgc acaaagtggt ggtgtatgat
     3301 tttggcgtga agttcaatat cctgagaaac ctggcagagg cgggttttgg ggtgacggtg
     3361 gtacctgcac agactcctgc agaaaaggtt ctggaaatga accctgacgg gattatcctg
     3421 agcaatgggc cgggagaccc ccagaccgtg acctatgcca tcgagaatgc aaaaaagctc
     3481 atgggaaaga agccgatctt cgggatatgt ctcggccacc agatattggg gctcgccatg
     3541 ggaggcagga catacaagct taaattcggc catcacggcg gcaatcaccc tgtgaaggat
     3601 atttccacag ggcgggtcga gatcacctcc caaaaccata actattgtgt ggatatcgtg
     3661 agtgtggggg gaaaagcaag gctgacgcac aaaaatctct atgacgggac agaagagggg
     3721 atggagcatg tggagctgcc tgtattctcg gtacagcacc atcccgaggc aggaccagga
     3781 ccgaacgatt ctgtgcatct gttcaggaga ttcagggaga tgatagagag aagtacatgt
     3841 taaaggaaat cacttcggat atttaccatc tcctccatcc caagatggca ttctttctca
     3901 cgagcgcgga caagaagggg aatcccaatg tcatgacctg tgcatgggca acgccggtga
     3961 gcgaagagcc tcctcttgtg gtcgtttgtg ttgcaaagga gagctatact gcggagctca
     4021 tcaaacagac aaaggagttt gtgatcaata ttcccacaaa gaagctcttg aaggcactat
     4081 gggtctgcgg caagacctcc ggacgggatg tggacaaatt caagaaggcc cgattgaagg
     4141 gtattggcgc tataagggtg aaaccttttg tggtagaggg atgcatcggg tatatagaat
     4201 gcagactgtg gaaggctgtg gatgcgggcg agtgttatgc attctttgga aaggtcctgt
     4261 ccgcatacgg tgatgacaac tacatcgaaa aaggtttatg gacgaagagg gcggaaatcc
     4321 ccctgcacct ggggggaagc aggatcgtct atttcaaata gcggcaaagt gtcggcggag
     4381 gcctatcaga ttgagaaaag cgagaatggt gtggtggtga gaaccacttc cttcaggaca
     4441 gagcgcagga gtgtgctcca tagcggcatc tttaaccgcg agcttgcatc cagccttgcg
     4501 gccggtgccg tgatggtcat ggtgggcttt ttttttgccc atcactttag gataaccgtt
     4561 gttcatttca tagcagtggc cttcgtgttt gcggtcctct tcatcctctt caggatatat
     4621 gcattcaggg aagcggtcct tgaaaccact tttgattggg aaaggaagac catcacgatt
     4681 tcactgaaaa gaaccatggg gagcaggcac cggtcatatc ccatggatgc gctggctggt
     4741 atcaggatcc atcacctctc aatgaagcca gaaaatttgg atgcagtgag gcttgtggag
     4801 aaggttgcgg tccagcatgg caccgtgata cctgggtttg gaaaggtgga agatttttac
     4861 tccgtggaac tggattttcg agaaaagaag attgttatat tcagcgccaa agaccagaag
     4921 ggggctgaat cttttgtgaa tgacctgaga agctctctgg ctggattcct tccttctgtg
     4981 gtgtgggaga cctaatgggt gcggtatatg atgagctcta tatctcgacc atgcgggaaa
     5041 tggatgtctg cgcaggtgcc atccggaagc tgaaaagagt aatcgaagac atggagacaa
     5101 aatacaatct gaagacccct gagttcgtgg agagattcag caaaggcgga atgaaagaca
     5161 acaaggattt tatggagtgg cgtggcagtt acgagaggct gaagcactgg gaggggcggt
     5221 tgaaggaatt taaagacatc ctccaaaatg caaaagatga atccgtgagg tgaatgtgcc
     5281 gaagagaacc gacctcagga agatactatt aataggctcc ggccctatcg tcatcggcca
     5341 ggcatgcgag tttgattact cgggaacgca ggcctgcaag gcgctgcggg aagagggcta
     5401 caaggtggtg ctcgtgaatt ccaaccccgc caccataatg acagatcccg agatggcaga
     5461 cgcggtctat atcgagcccc tcaccccgga gataatcgaa ctgattattg agaaagagag
     5521 acctgacgcc cttctcccca ccatgggcgg acagaccgcc ttgaaccttg ctgtggagct
     5581 tgccgaatca ggagttctcg gaaaatacgg tgtggaactc ataggcgcaa agctccctgc
     5641 cataaaaaag gcggaggata gggaactgtt caaagaggcc atggaaaaga tagggcttga
     5701 ggtacccagg agcgcgtata tagggagtgt gaagcagggg ctggattcca ttgagcacat
     5761 agggtttccc gccatcctga ggccatcctt cacccttgga gggaccgggg gaggcattgc
     5821 gtacaacatg gaagaatata tggaactcct ggaacgggga ctgaaactca gtcctgtcca
     5881 tcagatcctt gtggaggaat cggtcatcgg ctggaaggaa tacgaactgg aagtgatgcg
     5941 ggatatgaag gacaatgtgg tgattatctg ctctatagag aaccttgatc cgatgggaat
     6001 tcatacgggg gactccatta ccgtggcccc tgcccagacc ctcaccgaca aggaatacca
     6061 gaggatgcga gatgcatcca ttgcgatcat ccgtgagata ggggtggata cgggtggttc
     6121 caatatccag ttcgccctca gtcccgaaaa cggcaggatg gtggtcatcg agatgaaccc
     6181 gagggtatcc aggagttctg cactggcaag taaggcaaca ggattcccca ttgcaaagat
     6241 tgccgcgaag cttgcggtgg gactcagcct ggacgaaatc cctaatgaca tcaccaagga
     6301 gacccccgca tcctttgagc cgactattga ttatgtggtg accaagttcc cccgtttcgc
     6361 cttcgagaaa ttccctgagg cagacgccac ccttaccaca cagatgaaat cggtgggtga
     6421 ggcaatgtct atcggaagga cattcagcga atctcttcag aaggcgttaa gaagcctcga
     6481 gatcgggagc tatgggtttg aagaactcca ggtcagtgca agtgaactca aggcaaggct
     6541 gaagataccc aatgcagaaa ggatatggta tgtgacccag gcactcagga atggcatgcc
     6601 ggtaaatgaa atctataaac tcaccaacat agacccatgg tttcttcatc acataacgca
     6661 gattctggag ttggagaatg agataaagag ttatgcaagg gggataaagg aggaaaagac
     6721 caagagcctc gcaccgtatc tgttacccaa ggacctcttg acagaggcaa aggtgaaggg
     6781 tttctctgac aggaccattg caggactcct gaaggtcccg gagaaggaca tacgccatct
     6841 gaggaaacag tggaacttac atgcggtcta taagatggtg gacacctgtg ctgcagagtt
     6901 tgaggcatat accccgtacc tctattcgac gtatgagaaa cccttctccc gcatagcgca
     6961 cgtggcaggg cgcaaagagg aagaggagtc tggctctatg ccttacgcgt cctctgtgga
     7021 gaacgaatcg catcccacac acaagagaaa ggtgattatc ctcggctcag gccccaacag
     7081 gatagggcag ggaatcgaat tcgattactg ctgtgtccat gcggtctttg cccttaagga
     7141 actgggatat gaaaccataa tggtaaactg taatcccgag acggtgagca ctgattacga
     7201 tacctccgac aggctctatt tcgagcccct cactattgag gatgtcctca gtatcataga
     7261 ggtcgaaaag cctgacggag tgattgtgca gttcggaggc cagacccctc tcaagcttgc
     7321 ggttcctctg gaaaaggaag gggtgaagat actgggaacc tcccctgatt ccatagaccg
     7381 ggcagaggac aggaagagat tcaaggaact cctccatacg cttaacctca ggcaggctga
     7441 aagtgatact gccatgtccc cggaagaggc cctggagatc gcggggaaga tcggctatcc
     7501 ggtgatggtg agaccttcct atgtgctggg gggaagggca atggaaatcg tgttcgacga
     7561 aaattccctc gcggattata tgagacgcgc ggtgaaggcg tcccctgaac atcctgtgct
     7621 tgtggacaag tatctggagg acgcgattga ggtggatgtg gacgcccttt cggacggtat
     7681 tgacgtaatc gtcgggggcg taatggagca tatcgaagag gcaggcatcc attcagggga
     7741 ctctgcctgc tcattgcctc cctattccct cagtaattcc attgtggatg agatcaagag
     7801 acagacaaag gccctggcaa atgagttaaa agtcatcggt cttatgaata tccagtttgc
     7861 ggtaaaggag ggagagatat acatcctgga ggtgaatccc agggcatcaa gaactgtgcc
     7921 ctttgtctca aaggcaaccg gcgtacccct ggcaaagatg gctgcaaaaa tcatgggagg
     7981 gatgaccttg aaggaacttg gtcttacctc agagaaggag ataacccata tcgcggtgaa
     8041 agaggcggtg tttccttttg acaggtttca tggcgtagat accatcctcg gtccggagat
     8101 gaagtccact ggtgaggtga tggggattga tgaggatttt ggtctcgcgt atgcaaaggc
     8161 agaagaatcc tcacataaca aacttcccct ttcagggaac attttcatca gcgtgaagga
     8221 caaggacaaa cccggtacgt gcca
//
LOCUS       DATKOU010000266        22061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_2295005, whole genome shotgun sequence.
ACCESSION   DATKOU010000266 DATKOU010000000
VERSION     DATKOU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295005"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VMU21_12635"
     CDS             <1..285
                     /locus_tag="VMU21_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUO78411.1"
                     /translation="FQQAVIDVLVRKTEWAVTREGIHRVTVSGGVGANSALRKELARL
                     AKEKGIQLFIPSASLCTDNAAMIAAAGYYHVRAHDIAPMDMNPVAYLPLS"
     gene            326..2365
                     /locus_tag="VMU21_12640"
     CDS             326..2365
                     /locus_tag="VMU21_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78412.1"
                     /translation="MKGYIIIIGILAVIISALITLNIFFQQTLQMEMAEQFNKQQLLL
                     ANAEASNIQSYVNGLKDELSHIAVVVSGLKVKEKRDFSRLVTEFFKGKDMIKTDITFI
                     GSSGEVFFTKGKTSLETMNVKTMLEKTRGLCSEGMLVEQDSKRLRIAAPICRGDSFEG
                     AALLSLGIQDVAEEFLSPLKSGSRGYAWMMDGKGDLLYHPTQPGMVGKNLYKTDASCF
                     KCHRTFEAEKKIIEGKGDAYGRYVAPSGEDKVLAFSTASIGNSKWIVAVSAPYSEVTR
                     TIQRSMRFYSWLIISIFITTSIVSVVLIFFYRKRTKTEELEKHQKELEKYAEDLERKV
                     HKRTEELSAEKEKLNTIVSAIGSGIILLDNNGRIQWTNRTMQEMAGEDITGKYCEEIC
                     ADCTILGSYSVDEMQTEIVVNLFGRSDKYFQVTSVPIKGADGGVHGYIRLVQDITEMK
                     RMEEQMMHSEKLASLGRLTAGIAHEIGNPLTSVFSFVQILKDMEQDGFKKESLETIYF
                     HINRIAEILKQLSGFSKTPPVELKAWKVNSLIEASLSLIQYDKRAKDVTIVRDLGPDI
                     PEITTDGNQLSQVIVNLVLNAVDAMPQGGTLTIGSRVRNGSIVIDFTDTGVGIPKENL
                     GRIFDPFYTTKEKGTGLGLAVSYSIIKKLNGSLTVESEFGKGTKFVITLPIEGAK"
     gene            2362..3720
                     /locus_tag="VMU21_12645"
     CDS             2362..3720
                     /locus_tag="VMU21_12645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUO78413.1"
                     /translation="MKAKILVVDDEKDICKVLEFLLKSEGYEVSVAYNGEEALEKLKR
                     DHFDVVITDLKMDKVDGVAVLQKTKELSSDTAVVIMTAFASVESAVNIMKKGASDYIV
                     KPFLNEEIKITLRRILEQRRLVEENIALRQQLSQRMGYTDFVASSESMIRILETLEKV
                     IPTKSNILILGESGTGKGLIAEMVHRNSPRRDKPFISINCSAIPEGLLESELFGYKKG
                     AFTGAVSDKTGLITMADQGTLFLDEIGDMPMSLQSKILKVLESSEVTPLGDTRGRTVD
                     VRLVTATNHDLETCIKERTFREDLYYRLNVIEIKIPPLRERKEDIPLLVNHFLRRFSE
                     DNKKAIKGVESNAMGILMQYRWPGNARELRNVIERAVVLCQGDFITPDDFPEKLKREE
                     PALLGQGETSSLKLSLSEYEKNLILNVYHSQNNNKEETAKALGVDLATLYRKLKKYGI
                     EE"
     gene            3717..4010
                     /locus_tag="VMU21_12650"
     CDS             3717..4010
                     /locus_tag="VMU21_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78414.1"
                     /translation="MKKGFYIGSLCGGILGVIVALSMDLVLGGALGSGWREAVAHDLG
                     ALTGKTFGINSVVVLAGVAIVVGIIGAFGALVGGIFGVMLARLFSFLTKGDEA"
     gene            complement(4418..5074)
                     /locus_tag="VMU21_12655"
     CDS             complement(4418..5074)
                     /locus_tag="VMU21_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUO78415.1"
                     /translation="MSKIRVLIADDHTLVREGIAAFLKLSDDIEVVAEASDGLEAIEK
                     VKKFTPDVVLMDIAMPKLGGLEATVEIKKMNPDTKILVLTQYDDKEYISRFLKAGVSG
                     YLLKKAVGSELVSAIRAVNKNEFYLFSSIASKVVEGYLGKDKEAAMEDPYEKLTDREK
                     QVLKLVAEGYTHKEIGDMLSISTKTVIAHQTNMTEKLGISTRAGLIKFAIQRGIIKID
                     "
     gene            complement(5067..6893)
                     /locus_tag="VMU21_12660"
     CDS             complement(5067..6893)
                     /locus_tag="VMU21_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HUO78416.1"
                     /translation="MRRRITFSVVLSIFAILTALGIISYLSVHDSIQRSLQSRLTLAN
                     IIGKYFDYILETNLTRLYDISISGKIDFKDNDWESKKKALKTAYEYSIFTDRIFLTDL
                     QGNVVMTYPHREGGNVNLLNIPYVSRTLSERKPVISDVYTIEPTQRKVIFALVPLKSK
                     EGTIIGVAGGEINPTSYIFTHVIKSVSAGPSTNIELVDSHGTIIASNDPKRILTSSDH
                     NRFLGNLIAQKKSTVGTCHRCHSEEASHTTRTSDMLAFAPLSMAPWGISVREPQDIVF
                     SPSTTLRRGFFILSVISVITALLLGIGLSRGIVRPVQALITATQRIAKGNLTDPVTVS
                     SRDEIGILAKSFDTMRMKLSESLESIRRHNLELEHRVQERTQQIRLSRQKVESLLKKI
                     ISSQEEERRRIARGLHDEILQDLSAFLIKMDICKLYPEQLSMDKLEEMRGIALKSLDD
                     IHHVIQNLRPSVLDDLGLGAAVKWLLDKNLSEKGIRYYLTIDAEMEERFDPQVEITLF
                     RIIQEAIINIARHARAENVIVILRAQDHSVFVDIEDDGEGFDVHSMLRYTSDGGRGLG
                     LLGMKERTSLLDGKLEICSAPGSGTKISVRVPLGKPIEEANV"
     gene            complement(7040..7798)
                     /locus_tag="VMU21_12665"
     CDS             complement(7040..7798)
                     /locus_tag="VMU21_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HUO78417.1"
                     /translation="MMAEDTTAREKRIRRFCTPRIAEHWLAMLTFGTLVATGLAQSFF
                     SLDISRWIILKLGGIDNIRLLHRSTGAVFVLATLSHVVIAATGVVLRRWQPSMVINTS
                     DFMDAIHDIRYYLSMENSPAMCDRYTYKQKFEYWGILTGSFLMMVTGLVLWFPVLVAR
                     FFPGELIPVAKALHSNEALVIVLLIAVWHIYNSIFSPEVFPLDTSIFTGYISRERMIR
                     EHPIELARREGVQVEEIIEQGKAMHEMGSEEALP"
     gene            complement(7811..9277)
                     /locus_tag="VMU21_12670"
     CDS             complement(7811..9277)
                     /locus_tag="VMU21_12670"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUO78418.1"
                     /translation="MKGRMGIIVLLHFFLAATFSAEPAIARETVLSRDLTALSDDARS
                     CLGCHGKQGAVKTFENGESVSAYVDAKKFQASAHSFLTCAQCHTEFSANNHPSRRFRS
                     KKQYQITSSLACRHCHPDEQIKGKPIHANLLRGEKEGKLSLCTECHGAHYIMPVGGGK
                     VFAGEEQYCLRCHGHGLSMTFKDGGPQSLFIDKSLLEGSVHNKLSCWDCHYGFSSEDH
                     PRRTFGSKREYVIASSDSCRRCHFDKYSKTLESIHYATLSKGNLKAPVCIDCHGSHSI
                     AHGSKDRAVTTKGCQQCHPGIYSIYAASVHGKALIDEHNQDVPVCIDCHTAHNMTQTH
                     TLDYRERIPEICSSCHATKAVVEKYGLSTDVVKTYLTDFHGVTLGFYKRQRETLEKPA
                     RPIAVCTDCHGTHNIISTRGTDAALLKANLAKRCQQCHKDAPKNFPDAWLSHYEPNMR
                     SAPLVFIVNLVYKIFIPILAVGLVLQILLHIWRYAVDR"
     gene            complement(9491..10744)
                     /gene="nrfD"
                     /locus_tag="VMU21_12675"
     CDS             complement(9491..10744)
                     /gene="nrfD"
                     /locus_tag="VMU21_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HUO78419.1"
                     /translation="MNNEAVARPEQNFRAWFMDKLLMGMTWPEYRKSLITPFNIVAAL
                     ILSVGLPLMAYRFIFGLAAITDASHDYPWSLFLCWGLFSGVPLSATGFVMGTAYYIFG
                     FRRYAPLLRLALLAGLLGYFFAVVYLLMDLGRPWRIYYPMIIDFGTASVLFLVAWHVA
                     LYLSVQILEFSPSILEWVKSRRVRRWAMNITIFLTIAGIVLSTLHQSALGAMYLLMPG
                     KVHPLWYSSYIPIFFLSSSIFAAISMIIVVSTLSARFLRHRCDNHFLSSVDSLTVGLA
                     KGAAIAIYVYIVLKVIGIAHDMNWHYLNTPYGYWFLVEMLGFVLLPGLLFSLAVKKNS
                     AGIARFAAVFAVVGVVLNRMNFTLITFNWNLPHHLHEIIPRWREFAVVLSIATIHILI
                     FRWILNRMPVLSEEPGYPDAHYRQE"
     gene            complement(10737..11717)
                     /locus_tag="VMU21_12680"
     CDS             complement(10737..11717)
                     /locus_tag="VMU21_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUO78420.1"
                     /translation="MGKSSQNPDEHNGEKKTPRTITRRCFLGTVGALSTTALIGTGSK
                     ASAAGNFEGWPNRFGVLTDLTACVGCRSCEKACNEVNNLPSPKVPFDDKSVFNEPRRP
                     NQNAYTVVNQYANPKDKDKPIYRKLQCNHCEEPACANSCPIHAYNKTPEGAVLYREDF
                     CFGCRYCMAACPFNVPAFDYWSALEPKIMKCTMCYPRIKNGGIPGCVEACPAGALTFG
                     KRDLLLKLAAEKIAKNPDKYVDHIYGQREAGGTSWIYISGVPFGELGFPTNLPNKPLA
                     EETKGFLGAVPVILTVWPALFGMCYAALRHRDQIEKEKSANEKKEEVTHE"
     gene            complement(11732..12196)
                     /locus_tag="VMU21_12685"
     CDS             complement(11732..12196)
                     /locus_tag="VMU21_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUO78421.1"
                     /translation="MKRGLVLFFVITLSLFFAVSLYAGSSGKPPDVVSLDTMKDKYEA
                     VPFTHKKHADMISDCGKCHHQHGNNAADPCKDCHAVTPSVFKQSVTAGFLPCRNCHTS
                     PNPQKPHLVSLKVAYHSKCFECHRGMGNIGTDPKGCTELCHAIKGTKLSSSK"
     gene            complement(12302..12697)
                     /locus_tag="VMU21_12690"
     CDS             complement(12302..12697)
                     /locus_tag="VMU21_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78422.1"
                     /translation="MNTLFDFMTHIKGVEYILALLFIAGYILYSEVLKPRPFKTMIDT
                     GKDDIEHIRKTGFRNTLKVIGKIAAAPFIGIAYVVVLPFAFFAALGSAAMNGVMNLAG
                     KTAVFGWRPTEAYFTGKKKKKEEKKEEKN"
     gene            complement(12758..13123)
                     /locus_tag="VMU21_12695"
     CDS             complement(12758..13123)
                     /locus_tag="VMU21_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78423.1"
                     /translation="MLKYKGGQKVGKGTYWNFSNGTRVDIPDEGILPGNGRATYRRFP
                     PGIVLLAGPVIGLFYVIALPFMAVGTVLALVGKKLFSSMFNVLGNLVSFGWRPSEAHL
                     AGKRKKHKGMGGDQKKKEE"
     gene            complement(13436..13969)
                     /locus_tag="VMU21_12700"
     CDS             complement(13436..13969)
                     /locus_tag="VMU21_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78424.1"
                     /translation="MNVRKRVFGFLYFAVTIAILIGVLKVTNWLPTVLQEGIIRTYSS
                     IEEVKSRLKVRDIYIPSYFPQKFKWPPSRILAQSKPFVAVVMEFRNVETGETDLIISQ
                     AASRDFAPDKKIVISQIKERVNYPLNGRPSILEVGVCKNDVPCSRISWNEGSNEIRVE
                     MKSAPFELIKIAESMIH"
     gene            complement(13980..14930)
                     /locus_tag="VMU21_12705"
     CDS             complement(13980..14930)
                     /locus_tag="VMU21_12705"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HUO78425.1"
                     /translation="MAQAFCHSVIAALIVDRAIHAWRISDPLIRQRFSLIVMVSPILC
                     FPVYQIVNPDRGSLSFRLEALFDITRWLNMELWAKFPLSILFILILSITTLVFLFQEM
                     IPIVKHTIETWSAPEEVTTHDDPVIRKALELIPGEKPLISLIDDDDELILFSTTVKTP
                     TIFLSTGLIRGLSVDEIQAAIAHEIAHIRRNRRPLLIIIYLIRVLMFFNPVVLIEFRR
                     IVQEEEKVCDDIAVSLTKKPHALAEILEKFNKKTEALPLDKMKNLSALSTVVEDYSHN
                     IHMESRIIRLEKGTGLQTGGQWGRFFVTVVLIFVINYFIV"
     gene            15099..15809
                     /gene="ccsA"
                     /locus_tag="VMU21_12710"
     CDS             15099..15809
                     /gene="ccsA"
                     /locus_tag="VMU21_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HUO78426.1"
                     /translation="MVTAVYALPFYLLGLWKRVFLYVGLILQSMYIISRGLHLGRLPL
                     VGPHDTLIFLSAAIVAFSIPFNRGTKGDPALSRGISFTAMVFTASGILWQPHNAPLPP
                     VLKTLWFELHVVLAFFSYALFGIGAALGAFSLREKDVYHEALQYRAILIGYCLFSLSM
                     ILGGIWAYLAWGTYWLWTPKELWTSIVWLFYTLYLHARLMQGWAGRPLASLGILGFLV
                     VMFTYLGVSLFMKSSHTF"
     gene            15813..16550
                     /locus_tag="VMU21_12715"
     CDS             15813..16550
                     /locus_tag="VMU21_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78427.1"
                     /translation="MAVRAVIGFFVSLKTSLWLLCLVTVLLFAGAFIMPGEKAVQSIH
                     SMPLLEWMREQPVKSTWWLWSSMGLLLVLAANTLVCSIDSIAKKRKVTQWMLLISPQI
                     VHIGFLFMLIAHLVSAVGGFKTFAVAAEGTSLEMPDNSVLQIKGIAMSFDSYGYLYDW
                     SVDVEYRDNGQVLKKERLLPNKPVFLKGVGVYVKDLRAFPKKMVLLEVSREPGAVWAL
                     VGGILFMTGTIALLVLKMKREEIQTQP"
     gene            16992..17255
                     /locus_tag="VMU21_12720"
     CDS             16992..17255
                     /locus_tag="VMU21_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HUO78428.1"
                     /translation="MKFANVRELKNKTSEILRKAEKEAVIITSKGKPRAMVTAISEED
                     FEDYLLETSPGLQSTLAEALAEYHAKGGVPLDDYLAKRKKRRG"
     gene            17248..17529
                     /locus_tag="VMU21_12725"
     CDS             17248..17529
                     /locus_tag="VMU21_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HUO78429.1"
                     /translation="MDKFEVILSPHAVKDLDGFSDSVCKKIAYALKTLTENPFPRGKL
                     IRKLKGKNADFYRLRVDKYRVFYVIESGRVVVLRVLSKKDAERYIRNLN"
     gene            complement(17657..19105)
                     /locus_tag="VMU21_12730"
     CDS             complement(17657..19105)
                     /locus_tag="VMU21_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUO78430.1"
                     /translation="MRIFCLQYKELLQPYNQWWDNPEEALKTLPTFRRPIFDEIFRGL
                     KETPQVISITGPRRVGKSTLIKQVVKSLIAESVSPSHIIYYSMDDPALYRSGIDHASF
                     FDALMDYARNRTGEQVLYLFLDEIQRFERWELFLKKYYDLHYPVRFTISGSASSPIFR
                     KSRESLLGRIKDYHMLPFSFREFLLYKNRADEQMVGRLEEIYKTGEAVMGMLTKHPEY
                     ADLKKVTIPAVATDLRSSIDAGLSEFLMEGGFPEVWTLPDWETKQSYLFDNHVEKVIL
                     EDLVLAVELRKPERLKRFYISLLETPGREINWQQLSQELNINRATIEKYFPMLEMTDL
                     IHHVEKFTKSPLKVRRGNVKCYLVDLALRNAVLRLQETLLQDTNTLGLYAENLVFNAL
                     RKWEGTVSLSYYREKTYEVDFIVHTGAGTYLPFEVKYKENIENAELRILRNICDKLKC
                     SKGIVVTKKWDDFGVKENLFYMPLPHFLLLFD"
     gene            complement(20061..20270)
                     /locus_tag="VMU21_12735"
     CDS             complement(20061..20270)
                     /locus_tag="VMU21_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HUO78431.1"
                     /translation="MYKYEVIVYWSDEDEAYIAEVPELPGCMADGLTYEAAIKNAQVV
                     ISEWIETAKKLKRDIPKPKGRLAYA"
     gene            complement(20263..20523)
                     /locus_tag="VMU21_12740"
     CDS             complement(20263..20523)
                     /locus_tag="VMU21_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HUO78432.1"
                     /translation="MGKDTLDKILSGLSDKNIRFEDLKKVITDFGFGCRTKGDHHIFA
                     KEGVAEIINLQPLKDGKAKPYQVRQVRNLILKYKLHKGAKNV"
     gene            20710..20907
                     /locus_tag="VMU21_12745"
     CDS             20710..20907
                     /locus_tag="VMU21_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78433.1"
                     /translation="MQTTAKKTAKHFRLDVSMIKSAQKILGSKTETEAIETALSEIIY
                     QERMRKFIEQTAGKYKFEGVN"
     gene            20904..21323
                     /locus_tag="VMU21_12750"
     CDS             20904..21323
                     /locus_tag="VMU21_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUO78434.1"
                     /translation="MKTKAVPDTSFLVEHFRKETVQEAFLNLTRYYHITFSSVVLMEL
                     LAGAFDPKERKLIDQIARNFTVLSVTERQWFRSGEVMLRLRRDKKIDRERVKSLLADI
                     LIAVSVRDIGAVLITRNAKDFKLIRGVCDFKYLVVEY"
BASE COUNT         5470 a         5274 c         5548 g         5769 t
ORIGIN      
        1 tttcagcagg cggtcattga tgtgttggtg aggaagactg agtgggcggt aaccagggag
       61 gggatacacc gggtgaccgt atcaggtggt gtgggagcaa atagcgcact gagaaaggaa
      121 ttggcgcgtc tcgcaaagga gaaggggatt caattattta ttccctccgc atctctctgc
      181 acggataacg cggcaatgat cgccgctgcc ggctattatc atgtgagggc acatgatatt
      241 gcccccatgg acatgaatcc tgtggcgtat ctcccgctct cctgatccct gtaccagggc
      301 tctctgtttc tgttatgata aaacggtgaa aggctacatc attatcatag gcatactcgc
      361 cgtcatcatc tcagcgctca ttacattaaa catctttttt caacagactc tccagatgga
      421 aatggccgag cagttcaata aacagcagct gcttctggcc aatgcggagg cttcaaacat
      481 acagtcctat gtcaatggtc tcaaggatga attgtcgcat atcgcggtgg tggtgtcggg
      541 actgaaggta aaagagaaga gggatttttc ccggctcgtc accgagttct tcaagggaaa
      601 ggacatgatc aagacagaca ttacgttcat cgggtcgtca ggagaagttt tctttaccaa
      661 ggggaaaact tctctggaaa caatgaacgt aaagacaatg ctggaaaaaa caaggggatt
      721 gtgctcggaa ggtatgttgg tagaacagga cagtaagaga ctccggattg cggcaccgat
      781 atgccgggga gactcttttg agggcgcggc gctcctttcc ctgggcatcc aggatgtggc
      841 agaggagttc ctgagtcccc ttaagtcggg ttcacgggga tatgcctgga tgatggacgg
      901 caaaggcgat cttctctacc atccaaccca gcccggcatg gtgggtaaga atttatataa
      961 gactgatgcc tcgtgcttca aatgtcatag gacatttgaa gcggaaaaga agattatcga
     1021 agggaagggc gatgcgtacg gcaggtatgt tgcgccatcg ggtgaggaca aggtccttgc
     1081 cttttccaca gcgtccatcg gcaactcgaa atggattgtg gcggtgtctg ccccctattc
     1141 agaagtcacc aggacgatac agaggtcaat gaggttctat tcctggctta tcatatccat
     1201 ctttattact acaagtattg tgtcggtggt acttattttc ttttatagga agaggacaaa
     1261 gaccgaggaa ttggaaaaac accaaaaaga actggagaag tacgcggagg acctcgagcg
     1321 gaaggtgcac aagagaacag aagagctttc tgctgagaag gaaaaactca ataccattgt
     1381 gagtgccata gggagcggta ttattctcct cgacaataac gggcggatac aatggacaaa
     1441 ccggaccatg caggagatgg caggggaaga catcaccggc aagtactgtg aagagatatg
     1501 tgcggattgt accattcttg gttcctattc tgtggacgaa atgcagacag aaatcgtcgt
     1561 gaatctcttc ggaaggagtg ataaatattt tcaggtaacc agcgtgccga tcaaaggggc
     1621 tgacgggggt gtccacgggt atatcaggct tgtacaggat attaccgaga tgaaaaggat
     1681 ggaagagcag atgatgcact cagagaaact cgcatccctt ggaaggctga ccgcaggtat
     1741 tgcccacgag atcgggaacc cgcttacctc ggtattttct tttgtccaga tattaaaaga
     1801 catggagcag gatgggttca agaaggagag ccttgagacc atctactttc atatcaacag
     1861 gatagcggaa atccttaagc agctttccgg tttctcgaag acgccccctg tggagcttaa
     1921 ggcatggaag gtcaacagtc tcattgaggc ttccctttcc ctcattcagt acgacaagag
     1981 ggcgaaggat gtcaccattg tgagagacct gggccctgat atcccggaga tcactactga
     2041 tggaaatcaa ctctcgcagg tgatcgtgaa cctcgttctg aatgcggtag acgccatgcc
     2101 ccaaggcggg acgctcacca tagggagcag ggtgagaaat ggcagcatag tcattgattt
     2161 cacagatacg ggagtgggta tccccaagga gaatctcggc aggatattcg atcctttcta
     2221 cactacgaaa gagaagggaa cggggttggg gctcgcggtg agctacagca tcatcaagaa
     2281 attgaacggt agcctcacgg tagagagtga attcggaaag gggaccaaat tcgtgattac
     2341 cctcccaata gaaggtgcta aatgaaggca aagatactgg tagtagatga cgaaaaggat
     2401 atctgcaagg tcctcgaatt tctcctgaaa agtgaaggat acgaggtgtc tgttgcctac
     2461 aatggggagg aggccctgga aaaactgaaa agggaccact ttgatgtggt tatcaccgat
     2521 ctaaaaatgg ataaggtgga cggcgttgcg gtgctccaaa agacaaagga attgagttcc
     2581 gacactgcgg tggtgatcat gaccgccttc gcgtctgttg aatctgcggt caacatcatg
     2641 aagaaagggg cgtccgatta tatcgtgaag ccctttctta acgaggagat caagattacc
     2701 ctcagaagga tactggaaca gaggaggctc gttgaagaaa atatcgccct cagacaacag
     2761 cttagccaac gcatgggata tacggatttt gtggcgagct cggagtcaat gatccggatt
     2821 cttgagaccc tggaaaaggt catcccgacc aagagtaaca tcctcatcct tggtgagagc
     2881 ggtacaggga aggggctcat tgccgaaatg gttcatcgca acagcccccg gagggacaaa
     2941 cccttcattt ccatcaactg ttccgccatc cctgaaggcc ttcttgagtc agagctgttt
     3001 gggtataaga aaggggcctt taccggtgcg gtgtctgaca agacaggact tatcaccatg
     3061 gcagatcagg gaacgctttt cctcgatgag atcggtgata tgcccatgag ccttcagtca
     3121 aaaatactga aggttcttga atcttctgag gttaccccac tgggtgacac tcgggggaga
     3181 acggtggatg tgaggttggt caccgccacc aatcatgatc ttgaaacgtg tatcaaggaa
     3241 aggacattta gggaggacct ctactatcgg ttgaatgtca tagagataaa gattccccct
     3301 ctgagggaaa ggaaagagga tatccccctt ctcgtgaatc attttctcag acggttttct
     3361 gaagacaaca agaaagcaat aaagggggtg gagagcaacg caatggggat tctcatgcag
     3421 tatcgctggc ccggcaatgc cagggaactg aggaatgtga tcgagagggc ggtggtgtta
     3481 tgccagggag atttcatcac ccctgatgat tttccggaaa agctcaagag agaggagccc
     3541 gctcttttgg gacaggggga gacgtcatcc ctgaaactct ccctctcaga atatgagaag
     3601 aacctcatac tgaatgtgta ccattcgcaa aataacaaca aggaagaaac cgcgaaggcg
     3661 ctgggcgttg atctcgcaac cctctacagg aaattgaaga aatacggaat agaggagtga
     3721 agaagggatt ttatatcgga tccctttgcg ggggaattct gggagtgatt gttgcgctga
     3781 gcatggacct tgttctcggc ggcgcactcg gcagcggctg gagggaagcg gtcgctcatg
     3841 acctcggggc cctcaccgga aagacctttg ggattaactc agtggtggtg cttgcggggg
     3901 tggcgattgt ggtaggtatc atcggcgcat ttggcgcgtt ggtgggaggg atattcgggg
     3961 tgatgctcgc caggcttttc tccttcctca caaaaggaga cgaggcataa ttttacggac
     4021 acgtttcatg gcgagggatg tcaaccagcg gtccccccgc acttctgcgg ccttcagggt
     4081 ccaaagcttg gaagagggtt gttgcctacc aggttaacga gaggaggcac gcagtgtctg
     4141 actggaggcg gttaaacttg cacttgatta ccatacactg ttctgaacgg tagcaaaaac
     4201 ctccttttat taccacatgc accctgattc ctgtgttttc tcgatgagga caggggatat
     4261 ggagctttcc cgtcacctca gaatagtgct tctccgaata cacgctcctc atgccatatt
     4321 atacaatttt cgccgcggct atatgtgtgt cagacggagc ggggttgctc ttacaggaac
     4381 ggccaggagt ggaagatacg tgaaaggttg tttcacctca gtctatcttt ataatccctc
     4441 tttggatggc aaacttgata agccctgccc ttgtgctgat gccgagcttt tcggtcatgt
     4501 tggtctggtg cgcgatgacg gtcttggtgc tgatcgagag catatcgccg atctccttat
     4561 gcgtataccc ctctgccacg agtttgagta cctgcttttc cctgtcagtc agtttctcat
     4621 aaggatcttc catcgctgct tctttgtcct tccccaaata tccttcaacc acttttgatg
     4681 caatggaaga aaagaggtaa aactcattct tattcactgc tcttattgct gagacgagtt
     4741 cgcttcccac cgccttctta aggagatatc ccgaaactcc tgccttgaga aagcgtgaga
     4801 tgtactcctt gtcatcatat tgtgtgagga cgagtatctt ggtgtccgga ttcatcttct
     4861 ttatctcgac agttgcctcc agcccgccca gcttgggcat cgcgatatcc ataagaacca
     4921 cgtccggggt gaattttttc accttctcga ttgcttccag accatcggat gcctctgcaa
     4981 cgacctctat atcgtcggag agtttcaaaa atgcagcgat cccctccctt accagcgtat
     5041 ggtcatcagc aatcaacacc cgtattttag acatttgcct cctcgatagg ctttcccaaa
     5101 ggaacccgca cacttatttt tgttccgctc ccgggagctg aacatatctc caatttcccg
     5161 tccaggaggg atgtcctctc cttcatcccc agcagtccga gcccgcgtcc gccatcgctg
     5221 gtatatcgta acatggaatg gacatcaaat ccttctccgt catcttctat atcaacaaag
     5281 accgaatgat cctgcgccct cagtatcaca ataacattct ctgccctcgc atgtcgcgcg
     5341 atgttaatga tggcctcctg gatgatcctg aacagggtga tctcaacttg cgggtcgaat
     5401 ctctcttcca tttctgcatc tatagtgaga tagtaccgga tccccttttc gctcaagttt
     5461 ttgtccagga gccatttgac ggcggcgccg agtcccagat cgtcaaggac cgacggcctc
     5521 agattctgga taacatgatg gatgtcgtcg aggctcttta atgcaatgcc cctcatctct
     5581 tccagcttgt ccatactgag ctgctcaggg taaagcttgc agatatccat ttttatgaga
     5641 aatgccgata agtcctgcag gatctcatca tggagtccgc gggcgatcct tcgcctctcc
     5701 tcttcctgcg aggagatgat ttttttgagc aggctttcca ccttttggcg gctcagtcga
     5761 atctgctgtg ttctctcctg aacgcgatgt tccagttcaa ggttatgtct ccggatactc
     5821 tcaagggact ccgaaagttt catcctcatt gtgtcgaagc tcttggcaag gattccaatt
     5881 tcatccctgc tggataccgt caccgggtcc gtgagatttc cctttgcaat tctctgggtt
     5941 gcagtgatga gggcctgtac cggcctcaca atccccctgc tcaagcctat cccgaggagc
     6001 agcgccgtaa ttacagagat gacgctgaga ataaagaagc ccctgcgcag ggtggttgag
     6061 ggagaaaaga cgatatcctg cggttctctt accgaaattc cccatggggc catggagagg
     6121 ggagcaaagg cgagcatatc gctcgtcctc gtggtgtgag aggcttcctc ggagtggcat
     6181 cgatgacagg ttcctacagt gcttttcttc tgggcaatga gatttcccag aaatctattg
     6241 tgatcgctgg atgtgaggat tcgtttggga tcattggaag cgatgatcgt accatggcta
     6301 tccacaagct ctatattcgt acttgggcct gcggatacgg acttgataac atgggtgaat
     6361 atataactcg tcggattgat ctctcctcct gcgacaccga tgatagttcc ctccttgctt
     6421 tttaaaggaa caagggcaaa aatgaccttt ctctgtgtag gctcgatggt atagacatcc
     6481 gaaatcaccg gtttgcgttc cgaaagggtc ctgctcacgt aggggatgtt taacagattc
     6541 acgtttcccc cctcccggtg cggataggtc attactacgt ttccctggag gtcagtgaga
     6601 aaaatcctgt cagtaaaaat agagtattca tatgcggtct tgagagcctt tttctttgat
     6661 tcccagtcgt tgtccttaaa gtctattttg ccggaaatag agatatcata gaggcgtgtg
     6721 aggttggtct cgagaatata gtcaaagtat tttccaataa tatttgcgag ggtgaggcgg
     6781 ctttgtaaag accgctggat gctgtcatga acactgagat agctgataat gccaagggcg
     6841 gtgaggatgg cgaatatgct taggacaacc gaaaatgtga ttcttcttcg catgaatata
     6901 gtttaactta taatgagaag aatttgggga aagtcaaggg atgaatgggg gacacataac
     6961 gcgggtcagc tcagggagca aggggtgaaa aaatattcca aaccgagaat gagctgagat
     7021 catctgcaga gaagaagact taaggcaagg cttcttccga gcccatttca tgcattgcct
     7081 tcccctgttc gatgatctcc tccacctgca ccccttccct ccgcgcgagc tcgatgggat
     7141 gttcacgtat catcctttcc ctcgagatat atccggtaaa gatgcttgtg tcaaggggaa
     7201 atacctcggg gctgaatatg gaattgtaga tatgccaaac cgcgatgagg aggactatca
     7261 ccagcgcctc attggagtgc agcgcctttg ccacgggaat aagttcgcca gggaaaaacc
     7321 gggcaaccag gaccggaaac cacaggacaa gccccgtgac catcatgaga aagctgccgg
     7381 tgagaattcc ccagtattca aacttttgtt tataggtata acgatcacac attgcagggg
     7441 agttttccat gctgaggtaa tacctgatgt cgtgtatcgc atccataaaa tcgcttgtat
     7501 tgatcaccat tgacggctgc catcttctca gcacgacccc tgtggccgca atgaccacgt
     7561 gggagagcgt tgcaaggaca aacaccgcgc cggtagagcg gtggagcaac ctgatgttgt
     7621 cgattcctcc cagcttcagg ataatccagc gggaaatatc aagggagaag aagctttgcg
     7681 cgagccctgt ggctacgagt gttccgaagg tgagcatcgc gagccagtgc tccgcaatac
     7741 gcggggtgca gaacctcctt atcctctttt ctcgagcggt agtatcctct gccatcatat
     7801 ccctcctttt ctacctgtcc actgcgtaac gccatatatg caggagaatc tgcaagacga
     7861 gccctactgc gagaatgggg ataaagatct tgtacaccaa attcacgatg aagacgagcg
     7921 gggcgctcct catgttcggc tcatagtgag agagccaggc atcaggaaag ttctttggcg
     7981 catccttatg gcactgctgg cacctctttg caaggttggc cttcaggaga gccgcatcag
     8041 tgcctcttgt gctgatgata ttgtgggtgc cgtggcaatc ggtacacacg gctatgggac
     8101 gtgcgggttt ctcaagcgtc tcccgctgcc tcttataaaa tcccagtgtg accccgtgaa
     8161 agtccgtgag ataggtcttc accacatccg tggagaggcc atatttctcc acaaccgcct
     8221 tggttgcatg gcaactgctg cagatctcgg gtattctctc gcggtaatca agcgtgtggg
     8281 tttgagtcat gttatgtgcc gtatggcaat cgatacagac cggcacatcc tgattatgtt
     8341 catcaatgag tgcctttccg tgaacacttg ctgcatagat agagtagatc cccgggtggc
     8401 actgctggca ccctttggta gtcactgccc tatccttgct cccatgtgcg atggagtggg
     8461 agccgtggca gtcgatacag acaggggcct tcaggttccc cttgctgagt gtggcgtaat
     8521 ggatactctc aagggtcttt gagtatttgt caaaatggca gcgtctgcag ctgtcggagg
     8581 aggcaatgac gtattctctc ttagatccaa aggttctccg gggatggtct tcggatgaaa
     8641 acccgtagtg gcagtcccag cagctgagct tgttatgcac cgaaccctcc aaaagcgatt
     8701 tgtcgatgaa aagtgattgt ggtcctccat ctttaaatgt catgctcagg ccatgtccgt
     8761 ggcatctcag gcaatattgc tcctcaccgg cgaacacctt tccccctcca accggcataa
     8821 tgtagtgagc accgtgacat tcggtgcaca gagagagttt accttccttt tctccgcgta
     8881 acaaatttgc atggatgggt ttccccttga tctgttcgtc agggtggcaa tgcctgcatg
     8941 cgagggatga ggtaatctga tactgctttt ttgatctaaa tctccttgag ggatgattat
     9001 ttgcggaaaa ttcagtatga cactgtgcgc aggtcagaaa gctgtgggcg gaggcctgaa
     9061 attttttggc atccacgtat gccgaaaccg attcgccgtt ttcaaatgtc tttactgccc
     9121 cctgcttccc gtggcatccc agacaagacc gtgcatcatc agagagtgcc gtgagatctc
     9181 tggagagcac agtctctctg gcgattgcag gctcagcaga aaaggtggct gccaggaaaa
     9241 agtgcagcag gacgattatc cccatcctcc ctttcataaa cacccccttc cgaaagatta
     9301 aggcatgtga aaaggaagcc gaaaagtgtc cgagtcgata gagctgcgat cgccctatgc
     9361 cgggttctgt gcagtaaacg atacaaagga gagggcaggg gggtatctcc ccggctcaac
     9421 ccccctccct tcatcacaag ggagggaggg gggtacaggt gggtaccccg ccttattttc
     9481 cgcacgttct tcattcttgt ctataatgag cgtcggggta ccccggttct tcgctgagga
     9541 ccggcatccg gttcaatatc cagcggaaaa tgagaatgtg gatggttgca atagagagga
     9601 caactgcaaa ttcccgccag cgcggaataa tctcgtgaag atggtggggg agattccagt
     9661 tgaaggttat taatgtgaaa ttcattctgt taagaacaac ccctaccacc gcaaacacgg
     9721 cggcgaatct ggctatgccg gcgctgtttt tcttgaccgc aaggctgaac agcagaccgg
     9781 gcagaagcac gaaacctagc atctcgacaa ggaaccagta gccatacggt gtgttgaggt
     9841 aatgccagtt catatcgtgt gctatgccga tgactttcag tacgatatag acgtaaatag
     9901 cgatagcggc gcccttagcg agtccgaccg tcaggctgtc gacgctgctc aggaaatgat
     9961 tatcgcacct gtgcctcagg aacctggcag agagtgtact tacgactatt atcatggaga
    10021 tggcagcaaa gatgctggag ctcaggaaga aaatcggtat gtaggaggaa taccacaggg
    10081 gatgtacctt gcccggcatg agcaggtaca tcgcaccaag ggcagactga tggagcgtcg
    10141 agaggacgat acccgctatg gtcaagaaga tggtgatgtt cattgcccat ctcctgaccc
    10201 gccttgactt gacccattcc agaatcgaag gactgaattc caagatctga acggacaggt
    10261 acagagccac atgccatgcg acgaggaaga ggacagaggc ggtaccgaaa tcgatgatca
    10321 tcgggtagta tatgcgccag ggcctgccga ggtccatgag aagatagacg accgcgaaga
    10381 aataacccag gagtcctgcg agcaaggcga gtcttagcag cggggcatat ctcctgaacc
    10441 cgaagatata ataggctgtg cccatcacaa atccggtagc agagagaggg acaccgctga
    10501 acagtcccca gcagagaaag aggctccagg gataatcgtg ggatgcgtcg gtgatcgctg
    10561 caagtccaaa aatgaaccga taggccatga ggggaaggcc tacactcaat atgagtgcgg
    10621 caacgatgtt gaagggtgta atcagtgatt tcctgtattc aggccaggtc atacccatca
    10681 ggagcttgtc catgaaccag gccctgaaat tctgttctgg tctggctaca gcttcattat
    10741 tcatgtgtta cctcctcttt tttttcattc gcggacttct ctttttcgat ctggtctcga
    10801 tggcgcaggg ctgcatagca catgccgaaa agggccggcc atacggtcag tatcaccggg
    10861 actgcaccga ggaacccctt cgtctcttca gcgagcggtt tgttgggaag gtttgtcggg
    10921 aagcccaact cgccgaacgg taccccagag atatagatcc agctcgttcc acccgcctca
    10981 cgctggccat agatatggtc aacatactta tcgggattct ttgcgatctt ttccgctgcc
    11041 agcttgagca ataaatccct tttcccaaag gtaagggctc ctgccgggca ggcctctaca
    11101 catccaggta tgccgccgtt cttgattctt ggataacaca tggtgcactt catgatcttt
    11161 ggctctaagg cactccagta gtcaaaggca ggcacattaa agggacaggc tgccatgcag
    11221 tacctgcagc cgaagcagaa atcttccctg tacaggaccg cgccctcagg ggttttatta
    11281 taggcatgga tagggcagga gtttgcgcat gccggttcct cgcagtggtt gcactgcagt
    11341 ttgcggtaga taggcttgtc tttatccttc gggttggcat actgattcac cacagtatac
    11401 gcattttgat tgggccttct cggttcattg aagacagatt tatcgtcaaa cggaactttt
    11461 ggagagggga gattgttcac ctcgttgcag gccttttcgc aactccggca gcctacacat
    11521 gcggtcagat cggtgagcac tccaaaacga ttgggccatc cttcaaagtt acctgctgcg
    11581 gatgccttgg agccggtccc aatgagagcg gtggtgctga gggcgcctac ggtacccaaa
    11641 aagcatctgc gcgtgattgt gcgcggggtc tttttctccc cattgtgttc atcagggttt
    11701 tgagacgact ttcccataaa aacctccttt attattttga tgagctcagt tttgttccct
    11761 tgatggcatg gcacagttcg gtacacccct tcgggtcagt gccgatgttg cccatacccc
    11821 tgtggcattc aaaacacttg ctatggtatg cgacctttaa actcacgagg tgtggctttt
    11881 gagggttcgg tgaggtgtgg cagtttctgc agggcaggaa gccggcggtc accgattgct
    11941 taaataccga aggcgtcacc gcatggcagt ctttacaggg gtcggcagcg ttgtttccat
    12001 gttgatggtg gcacttcccg caatccgaaa tcatgtccgc atgtttcttg tgagtaaatg
    12061 gtaccgcctc atacttgtcc ttcatagtat cgagggatac gacatccggt ggctttcccg
    12121 aactaccggc atagagggag acggcgaaga acagggaaag ggtgataacg aaaaacagta
    12181 ctaaacctct tttcatttag gagcctcctt tttgttagtg taattcccgg cgaattgata
    12241 acgtccggca tccatccatc gggacttgct taggattctt gagtggtaca cgcttgacat
    12301 gctagttttt ctcttccttc ttctcttctt tctttttctt cttgccggta aaatatgcct
    12361 ctgtgggtct ccagccaaat actgccgtct tccccgcgag gttcatcact ccattcatcg
    12421 cggcagaacc cagggcagca aaaaaggcaa aaggaagcac gaccacatag gctatgccga
    12481 tgaacggagc tgccgcaatc ttcccaatga cctttagcgt gttcctgaat ccggtcttcc
    12541 tgatgtgctc aatatcgtct tttcccgtgt cgatcatggt cttgaaaggc cttggtttca
    12601 atacctctga atacaggata tatcctgcaa tgaagaggag tgccaaaatg tactccaccc
    12661 cttttatgtg tgtcatgaaa tcaaacagcg tattcataat taccctcctt tctctttagt
    12721 cattcctcta cggcacttta cgaataacta tctataccta ttcctccttc tttttttggt
    12781 ctccccccat tcccttgtgt ttctttctct taccggcaag atgtgcctca ctcggcctcc
    12841 acccgaatga cacaagattg ccgaggacgt tgaacatgct tgaaaataat ttttttccga
    12901 ccagcgccag gacagtccct actgccatga aaggcagtgc gatgacataa aacagtccga
    12961 ttacaggacc tgcaaggagc acaatgcctg gaggaaatcg tctgtatgtt gccctgccat
    13021 ttcccgggag tatcccttcg tccgggatat ctaccctcgt tccgtttgag aagttccagt
    13081 aagttccctt ccctaccttc tgtcctcctt tgtacttaag catcccactc accccctttc
    13141 ttattgcctc tgcgctgttc caaagttata tataacaaat gcaggggatg aatataattg
    13201 gaagaagttc ctattttctt tgggaattta tcctatatgt ctgaaaaaaa agaaaaaaga
    13261 tccctcgaaa tgcaggctgg gtggagtatt ttggccttac tgcgggattc ttgctgcagt
    13321 tttgtggttg ggcagggtgg aaaggatgta cgcacccttc cggacgggaa tgaaaggaaa
    13381 ggggatactg ccgctaagat ggcgttaaat cagggaaacc tctgggttca ttgtgtcaat
    13441 ggatcatgct ttcagcgatc ttaatcagtt caaagggagc ggatttcatt tccacgcgga
    13501 tctcattgct cccttcattc catgaaatcc tgctgcaggg cacgtcattc ttgcatacgc
    13561 ccacttccag tatggatggc cttccgttca acgggtagtt caccctctcc ttgatctgcg
    13621 aaatcactat cttcttgtcc ggggcaaagt ccctgcttgc agcctgggag ataatgaggt
    13681 cggtctctcc tgtctccaca tttcgaaatt ccatcaccac ggcgacaaag ggctttgact
    13741 gtgccaggat tcttgagggc ggccatttaa acttttgggg aaagtaagac gggatgtaaa
    13801 tatccctcac cttcagtctt gatttgactt cctcaatgct gctgtacgtc cttataatac
    13861 cctcctggag caccgtaggc agccagttgg taaccttgag cactccgatg aggattgcga
    13921 tggtcaccgc aaaataaaga aagccgaata ccctctttcg cacgttcacc ccttttccct
    13981 catacaataa aatagtttat cacgaagatg agcactaccg tgacaaaaaa cctcccccat
    14041 tgcccgcctg tttggagtcc ggttcctttt tcgagccgga taatcctgct ttccatatgg
    14101 atgttgtggc tatagtcctc aaccactgtg ctcaacgctg aaaggttctt cattttatcc
    14161 agaggaaggg cctctgtctt cttgttgaat ttttcgagga tttcggccag ggcatgtggt
    14221 ttcttagtca aggacactgc aatgtcgtcg cataccttct cttcttcctg aacaattctc
    14281 ctgaattcga taagcaccac ggggttaaag aacatgagta cccggatcaa atagatgatt
    14341 atcagtaagg gacgtctgtt ccttctgatg tgggcaatct catgggcaat ggctgcctgt
    14401 atctcatcaa cactcagtcc ccttatcagt ccggtggaca aaaaaattgt gggagtcttc
    14461 actgtggtgg aaaaaaggat cagttcgtca tcatcatcaa tgagagagat aaggggtttt
    14521 tcccctggga ttagttccaa tgccttacgg atgacaggat cgtcgtgggt ggttacttct
    14581 tcaggtgcag accaggtttc aatggtgtgc tttacaatag ggatcatctc ctggaacagg
    14641 aagacgaggg tggtgataga gaggatcagg atgaaaagga tacttaacgg aaactttgcc
    14701 cacaattcca tatttagcca cctggttatg tcgaagagag cctctagcct gaaagagagt
    14761 gagcccctgt cagggtttac tatctggtag accggaaagc agaggatagg ggataccatg
    14821 acaatgagac tgaacctctg gcgtataagg ggatcactga tgcgccatgc atggatggcc
    14881 ctgtccacaa tgagggcagc gatgacggaa tggcagaatg cctgggccat atacatgccg
    14941 gcgtaggaat taagaaagtg tgagaaattc atgttcatgg atttaaagga ttttttatca
    15001 tagcaaattc gtatctgttt tgacactcac acatctttta aggattgcat ttgtgtcaca
    15061 ctcctttcac gccgcttttc ctgtaaaata ggtctcttat ggtcacagcc gtttacgcgt
    15121 tgcctttcta tctcctgggt ctctggaaga gagtctttct ctatgttggc ctcatccttc
    15181 agagcatgta tatcatttcc cgggggcttc atctgggaag actccccctt gtgggtcctc
    15241 atgacaccct catctttctc tctgcggcaa tagttgcgtt ctccattcct ttcaacaggg
    15301 gaacgaaagg agaccctgca ctctccaggg gaatttcatt tacggccatg gtatttaccg
    15361 cttcgggaat cctctggcag ccgcataacg cgcctcttcc acccgtactc aagaccctgt
    15421 ggtttgaact tcatgttgtt ctcgcatttt tctcctatgc gctctttggg ataggagcgg
    15481 cactcggggc cttttctctc agggaaaagg acgtttatca tgaagccctc caataccgtg
    15541 ccatactcat tggttactgt ctgttttccc tgtccatgat tttgggcggc atatgggcat
    15601 atcttgcatg gggcacttac tggctctgga ccccgaagga actctggaca tccatcgtct
    15661 ggctcttcta tacgctctat ctccatgcaa ggctgatgca gggatgggcg gggagaccat
    15721 tggcatccct cggcatctta ggttttcttg tggtaatgtt cacctatctc ggagtgagcc
    15781 tttttatgaa gagctcacat accttttgag ccatggcagt aagggccgtg ataggattct
    15841 tcgtatccct aaagacatct ctatggcttc tctgtcttgt taccgtattg ctcttcgcag
    15901 gtgcctttat catgccggga gaaaaggcag ttcagtctat ccattccatg cccctccttg
    15961 aatggatgag ggagcagcct gtaaagagca catggtggtt gtggtcttcc atggggctcc
    16021 ttcttgttct ggccgcaaac acccttgtgt gcagcatcga ctccatagca aaaaagagaa
    16081 aagtcacgca atggatgctc ctgatctcac ctcagatagt ccatataggg ttcctcttca
    16141 tgcttatagc tcatctggtg agcgctgtgg gaggattcaa gacctttgca gtcgcggcag
    16201 aggggacatc cctggagatg ccggacaact cggtattaca gattaaagga atcgccatgt
    16261 cgttcgattc ctatgggtat ctgtatgact ggtcggttga tgttgaatac cgggacaacg
    16321 ggcaggtgct gaaaaaagag aggctcttgc cgaacaagcc ggtgtttctt aagggggttg
    16381 gtgtctatgt aaaggacctt cgggccttcc ccaagaagat ggtgcttctg gaggtgagca
    16441 gagagccagg cgccgtctgg gcactggtgg gaggtatcct ctttatgacc ggcaccatcg
    16501 cgcttcttgt gttgaagatg aagagggaag agattcagac acagccttag ggataatctc
    16561 cagccaatct tatccatgaa attctttctt ataggagagg catccggctc ttggcaagag
    16621 aatagccctt attctgtagg aagggtgata cgccttacac cgctttgttc ttatggaatg
    16681 tcaaggtccg cttgtgcgaa ccggcacata accccgactc ttgccgttcg ggccagatac
    16741 ctctcctgag ttaataaata tttgagaaat aacccagaca aattgacaga gattattgac
    16801 cgggtgtgga gttgaacaga agctttctca aggattctcg tcattttgag ggggatgaat
    16861 ggggattcga tcaaaccgca ataaagaagt gtccacgatt cctgcgaaac tgtatttttc
    16921 ttatgggtca ttgactatat ctaaaggctg tgtcatagta gctataaatg tagctacatg
    16981 gaggtgagtc aatgaaattt gccaacgtca gagaactgaa gaacaagact tcggagatat
    17041 taagaaaggc ggaaaaagaa gccgtaatca ttacgtctaa aggcaagccg agggctatgg
    17101 tgaccgctat ctccgaggaa gatttcgagg actatttgct cgaaacaagc ccgggccttc
    17161 agagcacgct tgccgaggcc ctggctgaat atcacgccaa aggcggcgtt ccgctcgatg
    17221 actatcttgc aaaaaggaaa aagaggcgtg gataagttcg aggttattct ctccccccac
    17281 gcagtaaagg atctggacgg ctttagcgat agcgtctgta agaaaatagc atatgcgctt
    17341 aagacactca ccgaaaaccc tttcccgagg ggcaagctga taaggaagct caaagggaag
    17401 aacgccgatt tctatagact cagggtggat aagtacaggg tgttttatgt tattgaatcc
    17461 ggcagagtgg tagtcttaag ggtcttgagc aaaaaggatg ctgaacgcta tatccgcaat
    17521 ctgaactgaa aaagcagtcg tgaagagggc tcgcaataaa cgcatcatat tgcacacgtc
    17581 gaatcatgaa agcatttacg cccaattcca caatcttcac ccgattgaga cgagggctgg
    17641 attatctcat tgggtgttaa tcgaagagaa ggaggaaatg ggggagaggc atataaaaga
    17701 gattctcttt cacgccgaaa tcatcccatt tttttgtaac aactatcccc tttgaacatt
    17761 tcaacttgtc gcagatattc ctgaggattc gtagttctgc gttctcgata ttttctttat
    17821 actttacttc gaaaggcaaa tatgttccgg cgccagtatg gacaatgaag tcgacctcat
    17881 aggtcttttc gcggtaataa ctaagagata cggtgccctc ccatttcctg agggcattga
    17941 aaacgagatt ctctgcataa agcccgagcg tatttgtgtc ctgcagcaat gtctcctgca
    18001 accgcaggac cgcattgcgg agagcgaggt caacgagata gcacttcaca tttccccttc
    18061 gtaccttgag cgggcttttg gtaaacttct ccacgtgatg gatcaagtcc gtcatttcga
    18121 gcatagggaa atatttttca atagtcgctc tgtttatgtt cagttcctgg gacagctgct
    18181 gccagttgat ctcacggccc ggggtctcaa gtaacgaaat ataaaaccgt ttcaatcttt
    18241 ctggtttccg caattccacc gccagcacca gatcctcaag gatcactttc tccacatggt
    18301 tatcaaataa gtaggattgc ttcgtttccc agtcgggtaa cgtccacacc tcagggaacc
    18361 ctccttccat gagaaattca ctaagaccgg catcgatgct ggagcgaaga tcagtagcga
    18421 cagcgggaat tgtcaccttc ttcaggtcgg cgtattcagg gtgtttcgtt aacatcccca
    18481 tcactgcttc accagttttg tatatctcct ctagtctgcc aaccatttgt tcatcagccc
    18541 gattcttgta aagcaggaac tctctgaacg aaaaaggcag catgtggtaa tctttgatgc
    18601 ggccaagaag gctttccctg ctctttctga agatgggtga tgatgctgaa ccggagatag
    18661 taaacctgac gggatagtga aggtcataat acttcttcag aaaaagctcc catcgttcaa
    18721 agcgctggat ctcgtcgagg aaaaggtaga gcacttgttc gccggtgcgg ttccttgcat
    18781 aatccattag agcgtcgaaa aaactagcgt gatctatccc ggaacggtat agtgcgggat
    18841 catccataga gtagtagatg atatgcgaag ggctcacgct ttctgcaatc aggctcttca
    18901 caacctgctt gatgagagta ctttttccaa ccctcctggg tccggttatt gagatgactt
    18961 gcggtgtctc cttcaatccc cgaaatattt catcaaatat cggacggcgg aatgtcggaa
    19021 gagtcttcaa ggcctcttca gggttatccc accattggtt atatggctga agcaactctt
    19081 tgtactgcaa gcaaaaaatc ctcactttcc ttttatgcta cttatgtata gcatatttgg
    19141 aaaaatatgt aaagaactaa ttatttcaac atgttatagg tagaatatat gggaagagca
    19201 gcctgcttta attcttttga ctcggttagt cagcagtgat tacgcgttat gatggcaaag
    19261 atttcagaat cgtttgtaat taaaaaaatt ggatctctcc ctgattgttc accaagaagg
    19321 attaggaaaa gttaccgaat tagtgaaccg ggtacatgtg tccccaatgt tcccccagag
    19381 aaattctctc gtcgttttct tccctcaaat taataggcgc tgaccctatt tttctatccc
    19441 agatttacgg acagagcgaa gcggcgtcgt agaatcggga ctgtccccgg ctaaagaatt
    19501 cggatatgga gagtgggggt atctggtggg gggtatcctc tttatgaccg gcacgattgc
    19561 acttcttatg ttgaagatga agagggaaga gacacggaca cagccttagg gataatctcc
    19621 agcccatctt atccatgaat ttctttctaa taggagaggc atccgcctct cggcaagaca
    19681 atagccctta ttctgtaggg gggggtgata cgccttacac tgctttgttc ttatggaatg
    19741 tcaaggtccg cttgtgcgaa ccggcacata accccgactc ttgccgttcg agccggatac
    19801 ctctcctgag ttaataaata tttagccagt cctctatttg gcggctggct tcagcaaata
    19861 acgttttttt acttcaatgg gtttcattgt ccaggtttgc acctttttct tggcatcacc
    19921 attatttgcc tcaataattc acaaaaaaga agtggcatcg atacctcctg aaaatttctt
    19981 gccccttact ctatcatggt cgtcataaag ataccatagt ttttagacag gctctgtgtc
    20041 cctatttgtt agttttattc ttatgcatat gccaatctgc cctttggttt cggaatatcg
    20101 cgcttcagtt ttttcgccgt ttcaatccac tctgagataa caacctgggc gttctttatg
    20161 gcagcctcat aggttagccc gtctgccatg cacccgggca gctcagggac ttcggcgata
    20221 tacgcttcgt cctcgtcgct ccagtatacg atcacttcat acttatacat tctttgcccc
    20281 cttatggagt ttatacttca gtatcagatt cctcacctgc ctgacctgat atggctttgc
    20341 cttcccgtct ttaagcggtt gaagattgat aatctcggca acaccttcct ttgcgaaaat
    20401 atgatgatcg cctttcgtgc ggcaaccaaa accaaaatca gtaatgacct tcttcaggtc
    20461 ctcgaacctg atattcttgt cggaaagccc cgacaaaatc ttgtctaatg tgtctttacc
    20521 catctaccca ttgataagta taccaaaagt tgctagttaa aaccctgccc cctttcttga
    20581 ttccgccggt tgactatgga acttcggact tctgaacttc gaaacgggtt gtgtccctgc
    20641 ttatttaact cccttgactt gtagccacag tatcatataa tgtatataca aatagggcaa
    20701 ggaggaacca tgcagacgac agcgaagaag acggccaaac attttagatt ggatgtgtca
    20761 atgatcaaga gcgctcagaa gatcctcggc tccaagactg agaccgaggc aatcgaaacc
    20821 gcgctttccg aaattatcta tcaggaaagg atgaggaaat ttatcgaaca gaccgccggt
    20881 aaatacaagt tcgaaggcgt caattgaaaa caaaggctgt gcctgatact tccttcctcg
    20941 tggaacattt cagaaaagaa acggttcagg aagcctttct gaatctcacc agatattacc
    21001 acattacgtt cagttccgtg gtgcttatgg aattgctcgc cggggccttc gatccaaagg
    21061 agaggaagct tattgaccag atcgcgcgaa attttactgt cctgtcggta actgagcgtc
    21121 agtggtttcg ctccggcgaa gtcatgctga ggctccgcag ggataaaaag atcgacagag
    21181 aacgtgtgaa gagtctcctg gcggacatct tgattgccgt gtcagttcgg gatatcggtg
    21241 cggtactcat cacgaggaat gcaaaggact tcaaacttat ccggggtgta tgtgatttca
    21301 agtatctcgt tgttgaatac taaaatgtcg ccttataagg cgaaagggcg catggcagag
    21361 aggttcaggc aatccatccc tattcttcag caggtgcggc ggcacgttaa ttcttgccgt
    21421 ctctctacca cggctctttt tcctgatgga cattgggcag cgggagagga acccgttaca
    21481 aaggcagata tgcggaataa tcatccttag ggaagattca agacctgacc gcgtttcctt
    21541 ctccacacat gagccgttaa gaaaatagaa tgtgtcccta gcaatattta ggtcactagc
    21601 ggtggtggtg aggttgagaa tcgccacttc tatgccatta gcttcattgc gtacttgcaa
    21661 gtgttccccg cataacagga tctcacagaa gccaacgcgg gtttcgacaa aacgaaaccg
    21721 ttgagttcgc cccgtagtat gtcacctctg gcctctccga cgttgtgacc ccgtcagatc
    21781 cctctttaat aggtcttgtt tcctgtgtgc gaaatgctgg ttgtaacatt cttttatcgc
    21841 tattccgctc tccgttccct cgtctgccgc ctccgctgag attgtacctt acaattgtag
    21901 tatgcagact ctggcaaaaa ccaaccgttt aacaacttgt aaggaggtat cagtatgact
    21961 atagaagaag ccatagaact ctttagaaag catcaaaatg ttaccgttaa gaagagtacc
    22021 ctcaagagct acggaaagtt tctggaacgg ttcaaagcaa g
//
LOCUS       DATKOU010000267        11581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3445636, whole genome shotgun sequence.
ACCESSION   DATKOU010000267 DATKOU010000000
VERSION     DATKOU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3445636"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VMU21_12755"
     CDS             <1..280
                     /locus_tag="VMU21_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78435.1"
                     /translation="MGTKRTPPPTPPKTATMPRRNVTTNNTSGQTHQGIAESGELGIG
                     GGSVDAHTEAANKWRMHNAATPMRYAVVFALGRVIFNSLDTISMVLPP"
     gene            694..1608
                     /locus_tag="VMU21_12760"
     CDS             694..1608
                     /locus_tag="VMU21_12760"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HUO78436.1"
                     /translation="MLDKTEKIWMDGEFVDWDNAKVHVLSHSLHYGLGVFEGIRCYDT
                     EKGPAVFKLKEHVKRLFGSAHIFLLEIPFSEREIEEAIVRTVKVNRVRECYIRPLVYI
                     GPGAMGVYAKGNPIRVSIAVWPWGAYLGEEGITKGIRIKVSSFIRGHVNSNLTRGKVC
                     GYYVNSQLAKKEAVSCGYDEALLLDTEGYVSEGSGENIFIVRDGILKTTPLTSILEGI
                     TRDSIMELARKEGIRAVEERFTRDEIYIADEAFFTGTAAEVTPIREVDGRIIGEGKPG
                     KITKKLQSIFFDVVKGRNKHYDSWLTYV"
     gene            complement(1678..2010)
                     /locus_tag="VMU21_12765"
     CDS             complement(1678..2010)
                     /locus_tag="VMU21_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUO78437.1"
                     /translation="MKGGEKRMKLLTILLTIAIAVMFIGSAMAVPPGKTVDWDTASGK
                     VVFDGKAHADKGLKCNDCHTKIFPMKKSTKMTMADMNAGKFCGECHNGTKAFATKDAA
                     NCGKCHKK"
     gene            2737..3924
                     /locus_tag="VMU21_12770"
     CDS             2737..3924
                     /locus_tag="VMU21_12770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUO78438.1"
                     /translation="MLSERARKIKPSPTLAMDAKAKALKAAGYDIVNFGVGEPDFDTP
                     ENIKEAAIRAVKEGFTKYTPVGGIDPLKDAIIEKFRKDNDLEYTREEIIASCGAKHSL
                     YNIAEALYGPGDEVIIPSPYWVSYPDQVLLNDAIPVIVKTHESDGFMLNPDALESHIT
                     EKTKAIILNSPSNPTGLTYDRRTLENIAEVVLKHNLVVISDEIYEKLVYEGAEHVSIA
                     SLGGDIKKRTLVVNGLSKSHAMTGWRIGFAAGPREIIKAMTNIQSQSTSNPNSIAQKA
                     AVEALTGPQDFIERMRNEFDRRRKFLVSELNAIPEVSCLSPTGAFYAFPNISGLFGKV
                     DGKPVFSSSDLALYLLEDANVALVPGDAFGNDSHIRLSYATSMENLKKGVERIRAAVG
                     KLK"
     gene            3935..4843
                     /gene="era"
                     /locus_tag="VMU21_12775"
     CDS             3935..4843
                     /gene="era"
                     /locus_tag="VMU21_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HUO78439.1"
                     /translation="MKGEDTAKDFRSGYVAIIGRPNVGKSTLLNSVLGEKVSIVTSRP
                     QTTRRRIIGIRTTGRSQIIFIDTPGFHKPQHKLGEFMVKEARESLKDVDIIVFMVEPR
                     SPGHGDTFIIERLKELGKTCPVLLLINKIDLVKKSELLPIMDEYHRCHPFDAIFPLSA
                     LNAEDVKLLVQDVEERLPHGPRYYPDDVVTDQYERFVVGEMIREKVMEVTEEEVPHSV
                     AVEIIQWTEREDGLLLLSANIYIERKGQKGIIIGKGGARLKAIGSAARADIEKFLARK
                     VFMKLWVKVKEGWRSDTRVLKDLGLH"
     gene            4856..5296
                     /locus_tag="VMU21_12780"
     CDS             4856..5296
                     /locus_tag="VMU21_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HUO78440.1"
                     /translation="MKVEGLLFDPRSSMYILLLKELEGNNTLPIWIGKPEADSIALAL
                     GGVMTPRPLTHDLIKNIVAGLKVKVTRIVITEILDNTYYALIHVADGKKEVPIDSRPS
                     DAVAVALRVNAPIFVEEGIMEKRSTDELEEWLKNLKPEDFGNVM"
     gene            5376..6122
                     /gene="recO"
                     /locus_tag="VMU21_12785"
     CDS             5376..6122
                     /gene="recO"
                     /locus_tag="VMU21_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HUO78441.1"
                     /translation="MLHRTEAVIFKTTAFSEADLIVTAFTLDYGLVNTFAKSSRKIKS
                     RFGSSLEPLTYAKVSFWGREESTLPRLTQSDIIKPFQSLRERNGNFFRVMEIIELTLG
                     LLAERETNRECFHLLLTTLNRIENSGSKNGAVFNTILYKIRLLDMVGYGPRLEGCARC
                     GTSGYNFYISHGSIICGTCAEGMDNPMRLSLGLIRLYDSLRKWDISKIDRIKMTGTLL
                     AELSEVLNSHIQYTIAKPLKTKAFLTGLKK"
     gene            6254..6781
                     /locus_tag="VMU21_12790"
     CDS             6254..6781
                     /locus_tag="VMU21_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78442.1"
                     /translation="MIVSAEIADFFCPNETCPDHGKRGMGNIILYNRYGRDHRRLLKC
                     RTCNFKFSERRNTFFFGLHTEESKIKEVITYLLDGKSFRETAVSAGIDKDTVLRIWKR
                     FVAYCEESMDGLLKEFNIRLEDLITLLYQRRGHPRKPCGSAISSLHKGSGTFWETLCG
                     DEEIAPRKGLCSEHE"
     gene            6774..7214
                     /locus_tag="VMU21_12795"
     CDS             6774..7214
                     /locus_tag="VMU21_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUO78443.1"
                     /translation="MSKWDPFRDLLFIQERVNRLFEDAFGAGESGGDVTLLGTWSPAV
                     DIYETETEFLVKAEIPEVKQSEIDIRVKGNTLSIEGERRPRRSMREGYHRVERVYGRF
                     QRTFLLPGSVDQERIAATLRDGILKIVLPKKEETVKKHIEIDHI"
     gene            7293..8396
                     /locus_tag="VMU21_12800"
     CDS             7293..8396
                     /locus_tag="VMU21_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HUO78444.1"
                     /translation="MGKIRVAIAGIGNCASSLIQGVDYYRRLGKGHSDRTLGLMHYNL
                     GGYKPFDIEFIAAFDIDKRKVGRPLKEAVLAKPNCTKLFNPHVSDYSVKVEMGEVLDG
                     VAPHMKDYPEERRFVVSERKPCDVPKVLRRSGADVLVSYMPVGSEKATAFYAEACLKA
                     GIGFINCVPVFIASDTQWARRFKERNIPIIGDDIKSQIGATIIHRMLTRLFEDRGVKL
                     DNTYQLNVGGNTDFLNMLNNGRLKSKKISKTEAVQSQLHTPLNNEHVHIGPSDYIPWL
                     YDNKVCFLRMEGRGFGHIPINIELRLSVEDSPNSAGVVIDAIRCLKIAKDRNVGGVLI
                     SPSSYFMKHPITQFSDEEARDMVEEFISGKRER"
     gene            8400..8963
                     /locus_tag="VMU21_12805"
     CDS             8400..8963
                     /locus_tag="VMU21_12805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUO78445.1"
                     /translation="MLSARLGHFLDKPLGPFAKKIAIHPNVLTIAGFVITVMAALVIP
                     VNLRLGGLLILLGGICDLGDGILARTNGRVSNFGAFLDSLLDRYSDSFLFLSIAWYLA
                     RMGDSTGAFLSIGTMVGAFLVSYARARGEGLGIQSQVGIMERPERIILLIVATLTGWL
                     APVLLVMLVLTHITVIQRAYVVWKAEQ"
     gene            9209..9409
                     /locus_tag="VMU21_12810"
     CDS             9209..9409
                     /locus_tag="VMU21_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HUO78446.1"
                     /translation="MAQGTVKWFNDSKGFGFITSEDGSDVFVHHTSIQGNGFKSLAEG
                     EKVSFDIEKGPKGPKAVNVVKL"
     gene            9606..10712
                     /locus_tag="VMU21_12815"
     CDS             9606..10712
                     /locus_tag="VMU21_12815"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HUO78447.1"
                     /translation="MLDQKSALPLLIIKGKKIGLPIIQGGMGVGVSLHPLARAVAREG
                     GVGIVSSACLDRLVSKRNGKKVNTYEAAYEEISLARSAGGFAGINIMLALGSTYEESV
                     KGALDAGADMIISGAGLPLSLPAIQAPGDTALVPIVSSARALDLICRKWERLRYRPDA
                     VVLEGPLAGGHLGFKLDEITLESNKLETLLPPVKDVARKYGDFPVIVAGGIFTHGDIL
                     RFLKMGADGVQMGTRFLATEESSATPEYKQAVLNAKEEDIIVATKPASPCGLPFRVIK
                     QCLSYVKERKPKCDKGYVLLKDAEGKYSICAAKQSNETNFCICNGLLSSAGYNSDVEK
                     PLYTVGTNACRVDKIVSVKTLMDELAGRVAVPAL"
     gene            complement(10734..>11581)
                     /locus_tag="VMU21_12820"
     CDS             complement(10734..>11581)
                     /locus_tag="VMU21_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HUO78448.1"
                     /translation="VSVRPVGQRELGTRTEVKNINSFKFVEKALEYEIKRQIRLLEEG
                     EKVIQETRLWDSSKGITESMRGKEEAHDYRYFPEPDLVPMTPTDEWVNEIRASLPELP
                     DKKRGRFISEYGLPEYDADMLVAERSTAEWFEEAVKMGGKPKNISNLILTEVFKYLKD
                     QGKDINETNLKPIHLVSLSGLVETAVVSATMAKSQVLYEIFNTGKMPEEIIKEKGLVQ
                     ISDSGAIEKAIDEVIAKNPKEAERFRAGDEKLISFFVGQVMKSTKGKANPQMVNEILK
                     KKLDG"
BASE COUNT         3030 a         2591 c         3048 g         2912 t
ORIGIN      
        1 gatggggaca aaaaggacgc cgcctcccac ccctcccaaa actgccacaa tgccgagaag
       61 aaatgtcacc acaaataata caagcggcca gacccaccag gggatcgctg aatcagggga
      121 actgggaatt ggcggaggat cggttgatgc gcataccgaa gcggcgaata aatggagaat
      181 gcacaatgca gccactccaa tgagatatgc tgttgtcttc gcattgggtc gcgtgatctt
      241 taattctctc gataccatat cgatggtcct tcctccttga gtttatccaa ttttttcgaa
      301 atcagtctga aatttacaac ggtcgctcct ttcacgaatc ttcatgcggg caccatgggt
      361 gctgtcagaa tcgttgaaga gcctgtggac agcgaattca ggcagccctt tgcgcagaca
      421 gggatccttc acagaagccc cttatggtct cgggaattcc gctgtatgat attataacga
      481 caggaagaga taaaaattaa tttttttaat gggattatta ggtgaacatt aaagtactcc
      541 cctgcccatt gattcgttgt gaaacaagta tcggactcga aaagtgagag aggtgttgct
      601 tcatggtgca gaaatttctc atgtttcccc cgcgcagctt cagcctaaga acagttgaca
      661 cttgtgtggc tccactggta taaatataca ccaatgcttg ataaaaccga gaaaatctgg
      721 atggacggcg agtttgtgga ttgggataat gccaaggtgc atgtcctgag ccattctctc
      781 cattatgggc ttggggtatt tgaaggtatc aggtgctacg acacagaaaa aggtccagcc
      841 gtcttcaagc tcaaagagca tgtgaagagg cttttcgggt cggcgcacat ttttttgctg
      901 gagattcctt tcagcgagag ggaaattgag gaagcgattg tcaggacggt caaagtaaat
      961 agggtcaggg aatgttatat aaggcccctg gtctatatcg gacctggtgc aatgggggtt
     1021 tatgccaagg gcaatcccat cagggtttcc attgcggtat ggccatgggg ggcctatctt
     1081 ggggaagagg gaatcaccaa gggaatcagg ataaaggtat catcgttcat acgagggcat
     1141 gtcaactcca acctcacaag gggaaaggtt tgcggctatt acgtgaattc ccagctcgca
     1201 aagaaggagg cggtatcgtg tggttacgat gaagccctgc tgctcgatac ggaggggtat
     1261 gtctcagaag ggagcgggga aaacatcttt atcgtaagag atgggatact caaaacgacc
     1321 cccctcactt ccattttgga gggtattacg agggacagca ttatggaact cgctaggaag
     1381 gagggtatcc gggcggttga ggagcggttt acaagggatg agatctatat cgccgatgag
     1441 gcattcttta cagggactgc agccgaggtg actcctataa gagaggtgga tggaaggatc
     1501 ataggggagg gcaaaccagg gaaaatcaca aaaaagttac agtccatatt ttttgatgtg
     1561 gtgaagggac ggaataagca ttatgactcg tggcttactt atgtttagaa agaactgaat
     1621 tggatgtaaa aaaaggcccg gcttcccggg ccttttttta ttagtttttt attctcacta
     1681 tttcttgtgg cactttccac agttggctgc atcttttgtt gcaaatgcct ttgtaccgtt
     1741 atggcactcg ccgcagaact tgccagcgtt catatccgcc atcgtcatct ttgtggattt
     1801 cttcattggg aatatctttg tatggcagtc gttgcattta agacccttgt cagcatgggc
     1861 tttgccatca aatacgacct tgccagatgc cgtgtcccaa tccacggtct ttccgggagg
     1921 aactgccatc gcgctgccga taaacatcac cgcaattgct attgttaata aaatggtcaa
     1981 cagtttcatt ctcttttcac cccctttcat tcttgagtgc tgtcatcctg acaggttgcc
     2041 tgaataaggt aattgcgaaa taaacaatta atcaaagcac tgaactataa taaatctctg
     2101 cgttattgtc aagcccccag taaacccgcg aaagggttac tttcatggaa tcgatacaac
     2161 cctagaaggg tcactcatgc agttccgtat acgacgatct tcaaaaattg ctcaccaagg
     2221 caggaaaaga aaataccccg tcaagagcat cttccggagc cgcagcgact tttcgcgtac
     2281 tatctgaagg tgtgcttatt ttttcttatg actggcgcaa aattttgatt taccactggc
     2341 cgaattcttg cacttctttc cttctttggt ctttgccgca catttcggtg tttttgctgt
     2401 tgccatagtg tcctccctgc tttttctttc actctaccat aagatggcat taatttaaag
     2461 tacgcttttg tgtgtattaa aaatttttat gtgggaagac gtgctggggt tgtgtcagca
     2521 ggataaatag tgcagcaaga aatgtggcga gtatggtcat gagcgataca gtaatgggcg
     2581 ccaatacaac ccactctttc tcctgtcagg cagccatgga gaatcaagga ggttgtcctc
     2641 ggcgcatggt tatttgaaca atagagaagc ggatgtgctc ctttattaaa gacattcaaa
     2701 tggtttacaa tagcaaaaat tctagggagg gtcattatgc tttctgaaag agcgaggaag
     2761 atcaagccat ccccgacact ggcaatggat gcaaaggcga aagcattgaa ggctgcaggg
     2821 tatgatatcg tgaactttgg tgtgggtgaa cctgactttg atacacctga aaatattaaa
     2881 gaagctgcca taagggcagt gaaagaagga ttcacgaaat atacccctgt gggcggcatc
     2941 gatcccctga aggatgcgat tatcgaaaag ttcaggaagg acaatgacct ggagtacaca
     3001 agggaagaaa tcatcgcatc atgcggtgca aagcacagtc tttataatat tgcggaggct
     3061 ctgtacgggc caggtgacga ggtgattatc ccttcccctt actgggtttc ttacccagat
     3121 caggttctcc tgaatgatgc gatccctgta attgtgaaaa cccacgagag tgacggattc
     3181 atgctcaacc ctgacgcgct ggaatcccat atcactgaga agacaaaggc catcatcctc
     3241 aactcgccct cgaatcccac gggcctcacc tatgatagga ggacccttga aaacatcgcg
     3301 gaggttgtcc ttaagcataa tctcgtggtg atctccgacg agatctatga gaaactcgta
     3361 tacgaaggtg ctgagcatgt gagtatcgcc tctctggggg gtgacatcaa aaaaaggacc
     3421 cttgtggtaa acggcctctc aaaatcgcat gccatgaccg gctggcgcat agggtttgct
     3481 gcgggcccaa gagagatcat taaggccatg accaacatac agagccagtc aacatccaat
     3541 cccaattcca ttgcgcaaaa ggcagcagtt gaagccttaa ccggcccgca ggacttcatc
     3601 gagagaatga gaaacgaatt tgacagaagg agaaaattcc ttgtctcgga gctgaatgcg
     3661 atccctgagg tgagctgcct gagtcccacc ggtgcctttt atgccttccc gaatatttct
     3721 ggactcttcg gaaaggtgga cggaaagccc gtgttttctt cctctgacct cgcattatac
     3781 cttcttgagg atgctaatgt ggctctcgtt cccggtgatg cctttgggaa tgacagccat
     3841 atccgactct cctacgcaac ttccatggag aatctgaaga aaggggtgga gagaataagg
     3901 gctgcagtgg gaaaacttaa gtgacgggcg agcagtgaag ggcgaagaca ccgccaaaga
     3961 ctttcgttca ggatatgtgg ccataatcgg cagaccgaac gtagggaaat caacgctcct
     4021 caacagtgtt ctcggggaaa aggtttccat tgtgacttcc cggccccaaa ccacccggag
     4081 gaggattatc gggatcagga caacaggacg cagtcagatc atttttatcg acaccccggg
     4141 cttccataag ccacagcata aactgggaga atttatggtg aaagaggcga gggagtcact
     4201 gaaggacgtg gacatcattg tcttcatggt agaaccaagg agtcctggtc atggtgacac
     4261 attcatcatc gagcggctta aagaactggg aaagacatgt cctgttcttc tcttgattaa
     4321 caaaatagac ctcgtgaaaa aatccgaact tctcccgatc atggacgagt atcaccggtg
     4381 ccaccccttt gacgccattt tccctctctc cgctttaaat gccgaggatg tgaagcttct
     4441 cgtgcaggat gtggaggaaa gacttcccca tggcccgagg tactatcccg atgacgtcgt
     4501 tacggatcag tatgagagat ttgtggtggg tgaaatgata agggaaaagg taatggaagt
     4561 aactgaggaa gaggtgcccc attccgttgc cgtggagatt attcagtgga ccgagagaga
     4621 ggacggactg ctccttttat cggcgaatat ttatattgaa aggaaggggc aaaaaggtat
     4681 tataatagga aaaggcggag cgagactcaa ggcgataggg tctgcagcaa gggctgatat
     4741 tgaaaagttc cttgccagga aagtcttcat gaaattatgg gtgaaggtga aggaaggctg
     4801 gcgcagtgat acacgggtgc tgaaagatct ggggctgcac tagatgctgg tgcgtatgaa
     4861 ggttgaaggc ttgctctttg atccaagaag cagtatgtat atcctgctcc ttaaggaatt
     4921 ggaggggaat aacaccctgc ccatatggat agggaaaccc gaggcagatt ccattgctct
     4981 cgcccttggc ggagtgatga ccccgagacc actcactcat gaccttatca agaatatcgt
     5041 cgcgggactc aaggtgaaag tgaccaggat cgtaattacg gaaatccttg acaataccta
     5101 ttatgcactc atacacgttg ccgatggcaa gaaggaggtt cccattgatt cgaggccaag
     5161 tgatgccgtg gctgttgccc tcagggtcaa tgcgcccata tttgttgagg aaggcattat
     5221 ggaaaagagg agcacggatg aactggaaga gtggctcaag aatctaaagc ccgaagactt
     5281 tggaaatgtt atgtagtgaa ttcggaatga agagactggg gccgaaacat tctgggactt
     5341 ctccattttt ttcgagaaag taatgtcgct gacttatgtt acaccgcact gaagccgtca
     5401 tcttcaagac cacagcattt tctgaggcag atcttattgt caccgccttt accctagatt
     5461 acggactggt aaataccttt gccaagagca gccgaaaaat aaagagcaga ttcggaagca
     5521 gtctggaacc cctcacgtac gccaaggttt ccttttgggg cagggaagag agtacccttc
     5581 ctcggctgac acagtcagat atcatcaagc cttttcagtc tttgcgggag cggaacggaa
     5641 acttctttcg ggtcatggag atcatagaac tcaccctcgg tcttttggca gagcgggaga
     5701 caaacaggga atgctttcat ctcctgctta ccacgctcaa cagaatcgag aacagcggct
     5761 caaaaaatgg cgcggttttc aatacgattc tttacaagat aaggctcctt gatatggtcg
     5821 ggtacggacc gaggcttgaa ggatgtgccc gctgcggcac gtcagggtat aatttttaca
     5881 tttcccatgg ctccataatc tgcgggacct gcgcagaggg catggataac cctatgaggc
     5941 tttccctcgg tctgatacgg ttgtatgatt ccctgagaaa atgggacatc tcaaaaatag
     6001 acagaataaa gatgaccgga acactccttg ccgagctttc ggaggtgctc aactctcata
     6061 tccagtatac catcgcaaaa cctctgaaga ccaaggcatt tctcaccgga ttgaaaaagt
     6121 aacgaagggc ggcgaccatg ccgcagcaga acttttctcg cctcagtccg tgcgtgatgc
     6181 ccgtatgggc atgtccccac tttattgaat atagtccttg acacgcacag gtatattagg
     6241 atataaaatc tgcatgatag tttcagcgga aatcgcagat ttcttttgtc ctaatgagac
     6301 atgccctgat cacggcaaaa gggggatggg caatatcatt ctttacaacc gttacggaag
     6361 ggatcacagg agactgctca aatgcaggac ctgtaatttt aagttctctg agaggcgcaa
     6421 taccttcttt ttcggcctcc acactgagga gagcaagatc aaggaggtta tcacttacct
     6481 gctggatggc aagagcttca gggaaactgc agtgagcgca gggattgaca aggataccgt
     6541 gcttcgcata tggaagaggt ttgttgcgta ttgcgaggaa tccatggatg gcctgctcaa
     6601 ggaatttaac atcagacttg aggaccttat caccctgctg taccaaagaa gagggcaccc
     6661 gagaaagccc tgtggttcag cgatatcaag tctccataaa gggtcaggaa ctttttggga
     6721 aacactctgt ggagatgaag agattgctcc cagaaagggg ctttgcagcg aacatgagta
     6781 aatgggaccc cttcagggac cttcttttca tccaggaaag agtgaacagg ctctttgagg
     6841 atgcctttgg cgccggcgaa agtggaggcg atgttactct gctggggacc tggtcgcccg
     6901 cggtggatat ctatgagacc gagacggaat ttctcgtgaa ggcggagatt cctgaggtga
     6961 aacaatccga gatcgatatc agggtcaagg ggaataccct tagcatcgaa ggcgagagaa
     7021 ggccgcgtcg ctccatgagg gaagggtacc acagggttga gcgagtctac gggaggttcc
     7081 agaggacatt tctccttccg gggtctgttg accaggagag gatagcggcg accctcaggg
     7141 acgggatcct gaagatcgtg cttccgaaaa aggaagaaac ggtcaaaaag catattgaaa
     7201 ttgatcatat ataagctcat atctgctatc aaagcgcctc tctgtgtgtt tgatggcatg
     7261 gtatcatcat cggatcactg aaaggaagac atgtgggaaa gatacgcgta gccattgcgg
     7321 gaatagggaa ttgcgcgagt tcattgattc agggggtgga ttactacaga aggcttggta
     7381 aaggtcattc agacaggacc ctggggctta tgcattacaa cctcggggga tacaagcctt
     7441 ttgatatcga gtttattgct gcgtttgata tcgacaagag aaaggtcggc agaccgctga
     7501 aagaagcggt attggcaaag cccaactgta caaagctctt taatccccac gtctcagatt
     7561 attctgtaaa ggtagagatg ggagaagtac ttgacggcgt ggctccccat atgaaggatt
     7621 atccagaaga gaggagattt gttgtctcgg aaaggaagcc gtgcgacgtc cccaaggtgc
     7681 tgcggcggtc tggtgcggat gtactggtga gctatatgcc ggtcggctcg gaaaaggcca
     7741 cggccttcta tgcggaggcc tgtctgaagg ccggtatcgg attcatcaac tgtgtccccg
     7801 tatttattgc ctctgatacg cagtgggcaa gacgttttaa agagagaaac atccccatta
     7861 tcggcgatga tattaagagc cagatcggtg cgactattat ccacaggatg ctgacgaggc
     7921 ttttcgagga taggggagtg aagctggaca atacctatca actcaatgtg ggggggaaca
     7981 ccgatttcct caatatgctc aataacggca gattgaagtc aaaaaagata tctaagacag
     8041 aggcagtaca atcacagctc catactcctc tgaacaatga acacgtgcac atcggtccat
     8101 cagactatat cccgtggctc tatgataata aggtatgttt cctgaggatg gaggggaggg
     8161 ggtttggaca tatccccatc aatattgaac taaggctgtc cgtggaggat tcacccaaca
     8221 gtgcaggggt agtcatcgac gcgatacgct gtctcaagat cgctaaggac agaaatgtgg
     8281 gaggggtgct catttctcct tcttcgtatt tcatgaagca ccctatcaca cagttttctg
     8341 acgaagaggc cagggatatg gtggaagagt tcatatcagg gaaaagggag cgatgagggg
     8401 tgctgagtgc gcgattaggc cattttctcg ataagcccct cggccctttc gcaaaaaaga
     8461 ttgcgattca tcccaatgtc ctcaccattg ctggatttgt cattaccgtc atggctgccc
     8521 ttgtcattcc tgtaaacctc agactcggcg gattgctgat acttcttgga gggatctgtg
     8581 atctgggaga cggtatcctt gccaggacaa acggtagggt gagcaacttc ggggcgttcc
     8641 ttgactccct cctcgacagg tattctgatt cctttctctt tctctccatc gcctggtatc
     8701 ttgcccggat gggagactct acgggagcct tcctgagcat cggcactatg gtgggtgcgt
     8761 tcctggtgag ctatgcaagg gcgaggggag aaggtcttgg aatccagagt caggtgggga
     8821 taatggaaag gcccgaaagg ataatcctcc ttatcgttgc cacactcacc gggtggctcg
     8881 cacctgtgct cttggtaatg cttgtcctta cccacatcac cgtaattcag cgggcgtatg
     8941 tggtctggaa ggcagagcaa taaggtgcgt cttctccgtg aggggatggc ataagcgcag
     9001 gagcatattc aagaaaagta taagcaatgt atacacgtta atcgtgtgag gaaattctct
     9061 tgacgaatgg tgtgaggaca tggtatcatt acttcagaat tttagaggtt tttgcgttca
     9121 caaaaggccc caaagacttt taactttgcg gccttttgtt tgtttgggcc ttctgagatt
     9181 taatttacag agaaggaggg agaatgaaat ggcacaagga actgtgaagt ggttcaacga
     9241 cagcaagggc tttggcttca ttaccagtga agacggcagc gatgtctttg tccaccatac
     9301 gtccattcag ggcaatggct ttaagagtct tgccgagggt gagaaggtca gctttgatat
     9361 cgaaaagggc ccgaagggtc ccaaagcagt caatgtggtg aaactctaat tcacggcgga
     9421 agccccctct gatgggggct tcttttctaa gagggaaatg aatcgcagga agaataaggc
     9481 acggatgcgg ctgagaccaa gcgatcatga gatacggtgt tcaaaagagt aacgtcggaa
     9541 aagcgaagag tcttcttatc tgagtatcct gcttaattct caacctacaa ctattttgga
     9601 gttatatgtt agatcaaaaa tcagcgttac cgctgcttat cattaaaggg aaaaaaatag
     9661 gtctgcccat tatccagggc ggaatgggag ttggagtttc actgcatccc cttgcccgcg
     9721 ctgttgcgag agaagggggc gtaggtattg tatccagcgc ctgtctcgac cggctcgtct
     9781 cgaaaaggaa tggcaaaaag gtgaacacct atgaggctgc ctacgaggag atctcactgg
     9841 ccaggagtgc gggaggtttt gcaggcatca acatcatgct ggcgctcggc agcacttatg
     9901 aagagtcggt gaagggtgcc ctggatgccg gtgcggatat gattatctcc ggggcagggt
     9961 tacctcttag tctccccgcg atacaggctc cgggcgatac tgcgcttgtc cccattgtat
    10021 cgtccgcgag ggcactcgac ctgatatgca ggaaatggga aaggctcagg tacaggcctg
    10081 atgcggttgt gctggaaggc cctctggcag ggggacatct cggctttaag ctcgatgaga
    10141 taactctgga atccaataaa ctggaaaccc tcctcccccc tgtgaaggat gtggcaagga
    10201 aatacggcga cttccctgtt attgtggcag gaggaatttt cactcatggc gatatccttc
    10261 ggttccttaa aatgggagct gacggggtcc agatgggtac gaggtttctc gctaccgagg
    10321 agagcagcgc aactccggaa tataagcaag cggtgctgaa cgcaaaagag gaggatatca
    10381 ttgttgccac caagcctgct tctccgtgcg gacttccttt cagggtaatt aagcagtgcc
    10441 tatcgtatgt caaggagagg aaaccgaagt gtgataaggg gtatgtgctt cttaaggatg
    10501 ctgagggaaa atacagtatt tgtgcggcaa agcagagcaa tgagacaaat ttctgcatct
    10561 gcaacgggct cttaagttcc gcggggtata attctgatgt ggaaaagccg ctctataccg
    10621 tgggcaccaa tgcctgcaga gttgataaaa tagtttctgt aaagaccctc atggatgaac
    10681 ttgcaggcag ggtcgcggta cctgctctgt aacggacact tgctttcagt ctcttagcca
    10741 tcaagctttt tcttcagaat ctcattcacc atttgggggt ttgcctttcc cttggtcgac
    10801 ttcatcacct gacccacgaa gaagctgatg agtttttcgt cacctgccct gaatctctcc
    10861 gcttctttgg ggttttttgc gatgacttcg tcaatggcct tttcaatggc gcctgaatcg
    10921 cttatctgca caagaccttt ctccttaatg atttcttcgg gcattttgcc agtattgaaa
    10981 atttcatata gtacttgtga tttcgccata gtagcgctta caacagctgt ttccacaagg
    11041 ccagacaagc tcacaaggtg gatgggcttc aagtttgttt cattgatgtc cttgccttga
    11101 tccttaagat atttgaagac ctctgtaaga atcaagttac ttatattctt aggtttacct
    11161 cccatcttta ccgcttcttc aaaccactca gctgttgatc tttcagcgac caacatatca
    11221 gcgtcatact cagggaggcc atattcggaa ataaaacgcc ctcttttctt gtccggcagt
    11281 tcgggcaggg atgccctgat ctcattaacc cattcatccg tgggcgtcat aggcaccagg
    11341 tccggttcag ggaaataccg gtagtcatgt gcctcctcct tgccgcgcat ggattcggtt
    11401 atcccctttg atgagtccca cagcctggtc tcttggataa ccttttcccc ttcctccagg
    11461 agacgtatct ggcgttttat ctcgtattcc agcgcctttt ccacgaactt gaaggaattg
    11521 atattcttca cctcggtcct tgtgcccaat tccctctgac ccacaggccg tacagagaca
    11581 t
//
LOCUS       DATKOU010000268         5625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3450116, whole genome shotgun sequence.
ACCESSION   DATKOU010000268 DATKOU010000000
VERSION     DATKOU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3450116"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VMU21_12825"
     CDS             <1..482
                     /locus_tag="VMU21_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HUO78449.1"
                     /translation="DFLNAGFLMLMPFTLNIGKYVDMLDVTQEKETLLNFLRMEKWIF
                     DSPGQAGECLRQFIRDCYQGNKLVKGELKVGEKLVDLKDITMPLLNIYASGDHLVPPA
                     ATRPLNDLVGSTDKSLYEFKGGHIGVFVGSKSQKELAPAISKWLHERANGETVKKNP"
     gene            668..1411
                     /locus_tag="VMU21_12830"
     CDS             668..1411
                     /locus_tag="VMU21_12830"
                     /inference="COORDINATES: protein motif:HMM:NF006072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUO78450.1"
                     /translation="MELAGKKVIITGGARGMGKRFAVDLKNLGAKPYVVDVVQENLDA
                     LKNETGIPGQVVDVSNEKSVEAFFESYTSEHGAPDVLINNAGITADALFIKQKGDEIT
                     KFPFSNWEKVINVNLTGVFLCAREAAFHMVKHKVNGVIINISSISRAGNLGQTNYSAT
                     KSAVDSMTVTWAKELSRYKIRVGAIAPGYINTEMVAKIRPDVLEKVIQSIPLGRLGEM
                     GEISQAAQFIIRNDFFTGRIMEVDGGMRI"
     gene            1475..2656
                     /locus_tag="VMU21_12835"
     CDS             1475..2656
                     /locus_tag="VMU21_12835"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HUO78451.1"
                     /translation="MEAYIVGGVRTAVGRFNGSLAGLDAIELGSAVIKEIIKRTSIKG
                     EEVEEVIMGNVLQAGLGQNPARQAAIRGGLPENIPSFTVNKVCGSGLKSVALAGQAIA
                     SGESSAIIAGGMEHMTSAPYVVKSARWGQRLGNTEMIDTLINDALWDMFYECHMGMTA
                     ENVAEKYHVTRKEQDEFAALSQKKTEQAIKEGRFKDEIVPLRLKQPKGEDKIFDTDEH
                     PRFGTTVENLAKLQPAFKQEGTVTAGNASGINDGAAAVLVVSKERMKELKPEWAFKIK
                     GSSSAALDPAYMGLGPINACKQLVGRARCTVNDLDLIEINEAFASQSIQVHREMGWDM
                     TRVNVLGGAIALGHPVGASGTRILVTLLYEMVRRDVNLGLASLCIGGGQGIAMIVERV
                     R"
     gene            3200..3952
                     /gene="tpiA"
                     /locus_tag="VMU21_12840"
     CDS             3200..3952
                     /gene="tpiA"
                     /locus_tag="VMU21_12840"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HUO78452.1"
                     /translation="MRKPLIAANWKMNKSIPEAREFTRLFLPSVSGIVDADIVIAPPF
                     TSLFAVAEKLTGTNVELSAQNAFYEEKGAFTGEISPRMVKDAGCSHVIIGHSERRQYF
                     QETDEIINKKIKTCQKHGLNVIFCIGESLAEREAGKTYDVLQGQIMDGLKEVNVQKLA
                     VAYEPVWAIGTGRSATPDQAQEAHHYIRTQLAKIYGAYADSIRILYGGSVTPENVDSL
                     MACPDIDGALVGGASLNPESFERIVKFKRRTI"
     gene            3949..4332
                     /gene="secG"
                     /locus_tag="VMU21_12845"
     CDS             3949..4332
                     /gene="secG"
                     /locus_tag="VMU21_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HUO78453.1"
                     /translation="MITLLLIIHILVCFFLIFIVLIQSSKGAELGAAFGGSSQTLFGS
                     RGAATLFSKLTTISAVVFMLSSLFLSMADIRSGSVIKKTVPIEQKAGAPAQQGPLQAP
                     QQGPVQAPMQNAPVQSPQQPPQPVK"
     gene            complement(4591..5523)
                     /locus_tag="VMU21_12850"
     CDS             complement(4591..5523)
                     /locus_tag="VMU21_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUO78454.1"
                     /translation="MEPTEPIARYRRFLKRRNYSVHTVRNYMYVLGQFFAWLSVPLQQ
                     ATFKETDAYMDYLLRRRRKPKTINCHLNCIHRFYEYLTEDEAIPLANPVRKNYRLRLP
                     RPLPRHLRDEQVAVLFKEMNNARDRAMFMLMLRCGLRVQEVARLTLDAIEHRSRQLCV
                     FNGKGGKDRIVYLSEDAQTALEEYMAKRRWSKERRVFLVRKGPLRGKPISVRGIQKRI
                     EYYAKTTGINVSCHHLRHTMATQLLNADADLVTIQDLLGHSKITTTQCYCRVSNLKVQ
                     RDYHKAINIVLQRTQPIRQNNSQWSKKRLSEHSI"
BASE COUNT         1455 a         1266 c         1520 g         1384 t
ORIGIN      
        1 gcgattttct gaacgcaggc ttcctgatgc ttatgccctt taccctcaac atcggaaaat
       61 acgtggacat gcttgatgtg acacaagaga aggaaaccct tctcaatttc cttcggatgg
      121 aaaaatggat atttgatagt ccgggtcagg cgggggaatg cctgaggcag tttatcaggg
      181 actgctatca gggaaataag cttgtgaagg gtgagttaaa ggtgggtgaa aagttggttg
      241 atcttaagga tatcacaatg cctcttctca acatatacgc gtctggcgac cacctggtcc
      301 ctcctgcggc aacaaggccg ctcaatgacc ttgtggggag cactgataaa tcgctgtatg
      361 aattcaaggg cggtcatatt ggtgtctttg tgggttcaaa atcacaaaag gagctggctc
      421 cggccatatc aaaatggctt catgaacggg cgaacgggga aacagtaaaa aaaaatccct
      481 aagacagcaa agtaccatag caaagcccaa gaacggcacc agaaaccaag ggagccgaac
      541 taagaagacc agccattagg cgggtactca caggaataca cgtcaacaaa gccgggcaag
      601 tattagggaa aaagatacaa aaggggagtc actacttccc aaaaaactat taaagggaga
      661 tatcatcatg gaacttgcag ggaaaaaagt cattataact ggcggtgcac ggggaatggg
      721 gaaaaggttt gcagtggatc ttaagaatct gggggcaaaa ccctatgtgg tggacgtggt
      781 tcaggaaaac cttgatgccc tgaagaacga gacaggtatc cccggacagg tggttgatgt
      841 gagcaacgaa aagagcgtag aagcgttctt tgaaagctac acctctgaac acggcgcgcc
      901 ggacgtattg attaacaatg cgggtattac cgctgatgcc ctttttatca agcaaaaggg
      961 ggatgaaatt accaaatttc ccttttccaa ctgggaaaag gtgattaatg tgaacctcac
     1021 cggggtgttt ctctgcgcga gggaagctgc ctttcacatg gtgaagcata aggtgaatgg
     1081 ggtgatcatt aatatttcct ccatcagccg tgcggggaat cttgggcaga ccaattattc
     1141 agccacgaaa tctgcggttg attccatgac cgtgacatgg gcgaaggagc tttcgcggta
     1201 caagattagg gttggagcca ttgcgccggg gtatatcaat accgagatgg tcgcaaagat
     1261 cagaccagat gtgctcgaaa aggtcatcca gagcatccct ctcgggagac tgggtgaaat
     1321 gggggaaata tcccaggcgg ctcaatttat tataagaaac gatttcttta ccggccggat
     1381 catggaagtg gacggcggta tgagaatctg attttttgtg gcagagtcat ttattcttgg
     1441 tgtttcataa actcgtaata gaggaggggt aactatggaa gcgtatatcg tgggtggcgt
     1501 gagaaccgcg gtcgggagat tcaacggatc ccttgcaggg ctggacgcca ttgaactcgg
     1561 ctcggcagtg attaaggaaa tcatcaagag aacgtcaata aagggtgagg aagttgaaga
     1621 ggtgattatg gggaatgtgc tccaggcagg gttgggacag aaccctgcgc gacaggctgc
     1681 gatccgcgga ggtctcccgg aaaatatacc ctcatttacc gtgaacaagg tgtgcggctc
     1741 gggattgaag tccgttgccc ttgcgggtca ggccatcgcc tcgggagaat ccagtgccat
     1801 tatcgcgggc ggcatggaac acatgaccag cgcaccctat gtcgtgaagt ctgcgcggtg
     1861 gggacagcgc ctgggaaata cggaaatgat agataccctc attaatgacg cgctgtggga
     1921 catgttctat gagtgccaca tgggaatgac cgctgaaaac gtagcagaga aatatcatgt
     1981 taccagaaag gaacaggatg agtttgctgc cctttcccag aaaaagaccg agcaggcaat
     2041 taaggagggc aggtttaagg acgagatagt tcctttgaga cttaagcagc ccaaaggtga
     2101 ggataagatt tttgatactg atgaacaccc ccgttttggt acgaccgtgg aaaaccttgc
     2161 aaagcttcag ccggcgttca agcaggaggg gactgtaact gcggggaatg cctctggcat
     2221 caatgacggg gcggctgccg tactcgtggt ttcaaaggaa aggatgaagg aactaaagcc
     2281 cgaatgggct tttaagatca agggatcatc atccgctgcc ctggatcctg catatatggg
     2341 gctcggtccc atcaatgcgt gcaagcagct ggtgggaagg gcacgctgta cggtgaatga
     2401 tcttgatctc attgagatca atgaggcatt tgcttcccag tcgatccagg tccacaggga
     2461 gatgggatgg gacatgacca gggtgaatgt tcttggcggc gccattgccc tgggacatcc
     2521 tgtgggagca agtgggacaa ggatccttgt aaccctgctc tacgaaatgg tacgacggga
     2581 tgtgaatctg ggactcgcct ccctctgtat aggcggggga caaggaattg cgatgattgt
     2641 tgaaagggtg agataaaagt tcagggtctg gtcaaagttc agagaccctt gcgccttgtg
     2701 gcaagcgtta caaccagacc gaggacgcca aagtgagtgt ccgggcgcct aaagggttac
     2761 gcgcaatgga aaatcaagaa aacttaagtc agacagtttg agcggggaga cccgtgatcg
     2821 ccggactcct ctcttaagcc ctcacttctg agatttattc ttctgaacca tttgaactac
     2881 tgcaaccact gccactacga gaacaacaac acagaatgcc accataatga tattcagaga
     2941 catagcgttc cctccttcga cgtaatcttt acaggcgtat acacttaagt tagcgattat
     3001 tttctgattt gtcaatgtga gatgaaactt cttgctgctg gttatcagtc tgagacggtg
     3061 tcatgttggg tccaatcagc ggcgtatcaa cgcatttacg caggtaactg tccggagtgg
     3121 acaattagac cgggtatcgg aaccacccgt tattccttga catttccctt acagatagga
     3181 ttaaatattt aattctatta tgcgcaaacc gttgatagca gccaactgga agatgaataa
     3241 gagcataccc gaggcaaggg agtttacccg tctgtttttg ccttctgttt cgggcattgt
     3301 agatgcggat attgtcattg ctcccccttt tacttcactt tttgcagtgg cagaaaaatt
     3361 gacaggaaca aacgtggagc tttctgccca gaatgccttc tatgaagaaa agggagcttt
     3421 tacgggagag atatctcccc gcatggtaaa ggacgcaggg tgcagccatg tgattatcgg
     3481 acattcagag agaaggcagt actttcaaga gacggatgag ataatcaaca aaaaaataaa
     3541 gacctgccag aagcacggtt tgaatgtaat cttctgcata ggcgagtcac ttgcggaaag
     3601 ggaagcgggc aagacgtatg acgtgctaca aggacagatc atggacggtc ttaaagaggt
     3661 gaatgtccag aaacttgctg tggcgtatga acctgtgtgg gccatcggca cgggcaggag
     3721 cgcgacacct gatcaggcgc aggaggcaca ccattacatt agaactcaat tagccaagat
     3781 atacggcgcg tatgcagatt ccatcaggat attgtacgga gggagtgtta ctcctgaaaa
     3841 tgtggacagc ctcatggcat gtcctgacat agacggcgcc ctggtgggtg gtgcgagcct
     3901 taaccctgaa agttttgaaa gaatcgtaaa attcaaaagg aggaccatat gattacacta
     3961 ctattgatta tccatatttt agtctgcttc ttcttgatat tcattgtgct gatacagagc
     4021 agcaagggcg ctgagctcgg tgctgccttt ggaggttcca gccagacact cttcggaagc
     4081 cgcggggctg caacactctt tagcaagctc acgacgattt ctgctgtggt atttatgctc
     4141 tcatctcttt tcctttccat ggcggatata aggagcggct ccgtcatcaa gaagacagtg
     4201 cccattgaac agaaggcagg cgcacctgcc caacagggac ctctgcaggc accccagcag
     4261 ggaccggtcc aggctccgat gcagaatgcg ccggtgcaat ctccacagca gcctccccag
     4321 cctgtgaaat aacacgtttt caatatcttg tgagggatag attgaattgt taggaacctg
     4381 ctctcttata gagagcaggt tcctaggatt ttgagtctcg gattacgcct ggcatgttcc
     4441 cagagcaagg ctgaatggta ttaatgtgcg ttcttgggat gtagggttcc tcattttact
     4501 gcaaaggtgc accccgcagc gtatccattc tttgtttgtt tcacccagct atgcgtaaca
     4561 taacctgctt ttaattttat tattagccag ttatatactg tgttctgata gccgtttttt
     4621 gctccattgg gagttattct gccttatcgg ttgagttctc tgcaagacaa tgttaatggc
     4681 tttgtggtag tctctctgca ctttgagatt ggagaccctg caatagcact gggtcgtagt
     4741 gatcttgctg tggcccagga gatcctgtat ggtcacgaga tcggcatcag cattcaatag
     4801 ctgggttgcc atcgtatgtc tgagatgatg acaggataca ttgatccctg ttgtcttcgc
     4861 atagtactcg atccttttct ggataccacg gaccgagatc ggtttccctc ttaatggtcc
     4921 cttccgcact aagaatactc ttctctcttt tgaccatctg cgctttgcca tatattcttc
     4981 taaggcagtc tgcgcatcct cactgagata gacgatcctg tccttgccac ccttgccatt
     5041 gaagacgcac agctgtctgc tgcgatgttc tattgcgtcc agcgtcagcc gtgcaacctc
     5101 ctggacccgg aggccacacc ggagcatgag catgaacatg gcccggtccc tggcgttatt
     5161 catctctttg aagagcacgg caacctgttc gtccctgaga tgtcggggca gcggccgggg
     5221 aagccgcaac cggtagttct ttctgaccgg attggcaagg ggtattgctt catcttcggt
     5281 caggtattcg taaaacctat ggatacagtt aagatgacag ttgatggtct tgggctttct
     5341 tctccttctc aggaggtagt ccatgtacgc gtcggtctct ttgaaggtcg cctgctgcag
     5401 aggaaccgat agccatgcaa agaactgacc gagcacatac atatagttcc ttacggtgtg
     5461 taccgagtag ttccttcgtt tgaggaatct ccggtacctg gcaatcggct cagtaggttc
     5521 catgaatgcc tccctgctcg ataaccgcca tggcacggaa gtactcctcc tcacggcttt
     5581 tgtcggtaag gcgtgcatag cgccgggtca tctctatctc acggt
//
LOCUS       DATKOU010000269         8510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3454398, whole genome shotgun sequence.
ACCESSION   DATKOU010000269 DATKOU010000000
VERSION     DATKOU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454398"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VMU21_12855"
     CDS             <1..409
                     /locus_tag="VMU21_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78455.1"
                     /translation="STTRPPPQTEAARRWSQSIISATLKSPLSEACPDALAVMDISIE
                     KPSISNRNPRESRYGLPAFNVIDKSMRVAMRMSQTDPKWVLRITVGSIGKAAETGWPL
                     ISEARISNSATLLATITPKQIPNNRHMEARIFP"
     gene            complement(930..1892)
                     /locus_tag="VMU21_12860"
     CDS             complement(930..1892)
                     /locus_tag="VMU21_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78456.1"
                     /translation="MTYRTKALTTVFSSGCVLCIALAIILLLPASSWAHGFAGERFFP
                     TTFQVDDPFISDEFSILINRVKQPGDEPNMLTQIDIDYSKRILPNFGLEFHEAYVHLG
                     SNGDGSANGWDNLGIGAKWQFLTNAPHELILSVGTDFDLGGTGAHQIADSFTTISPAL
                     FFGKGLGDLPESVKYLRPFAITGVIGPNFPTRSKNVLVDPDTGETDIERNPTTLTWAF
                     TLQYSLIYLQSFVKDIGLGAPFKRMILVTEFPMETCMSADCKGKITGTVNPGVVWCGK
                     YMEFGVAAQIPINSRSGHSVGILGLLHLYVDDLWPKGIGGPIFH"
     gene            complement(1935..3197)
                     /locus_tag="VMU21_12865"
     CDS             complement(1935..3197)
                     /locus_tag="VMU21_12865"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUO78457.1"
                     /translation="MKAYFCLLSFKRVIILAAVAFMALCNIALGWAWASEIGTYSWSK
                     EEISVIRSLWIGSLPPLPKDPSNAYADNPKARALGKRLFFDPRFSGNLQVSCATCHRP
                     DMNFDDDLPLAHGMGSTTRRTMPLLGVAYNTWFFWDGRKDSLWSQALGPIESNVEHGF
                     TRTAYAYLISKNYRNEYEEIFGKLPDITNAVATPATEDAVALKTWVSMTPDKREEVNR
                     IYANIGKAIAAYVRTIVPGPSRFDRYVEALQKGDTATMAISLTPEELNGLGLFIGKAK
                     CTNCHSGPLFTNSDFHNTGVPTPARMPYDKGRADGIRKVLSDEFNCLGRYSDAGPHDC
                     AELRFLDTKTDKYDGAFKTPTLRNVAERPPYMHAGQFTTLKEVLEFYRQSESHELGHR
                     TLSDEELRQLEAFLRSLSGPIITADSHQ"
     gene            complement(3211..3897)
                     /locus_tag="VMU21_12870"
     CDS             complement(3211..3897)
                     /locus_tag="VMU21_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HUO78458.1"
                     /translation="MVIKEKELKVGVNTLDIIVHDKSDKDVAGAEVTVTPWMPEMGHG
                     VYEKPVVRERGGGLYSVENIILIMGGRWELKIKIRKGDMEDSAVFDFPEVKVSQTSSA
                     DEHKATYSSAPADVDTSTVRMSAKKLFKVSYVSETVPIPVGRIIAWKLNVNTADGRPV
                     KDAVISINGDMPEHGHGLPTQPEVVKGTKDGDYIVQGLKFSMPGWWVITFTVKRQDLD
                     DTVTFNLVVQ"
     gene            complement(4175..4927)
                     /locus_tag="VMU21_12875"
     CDS             complement(4175..4927)
                     /locus_tag="VMU21_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78459.1"
                     /translation="MKSKVTLVSLLTVVVAVFLVVSGLVYAEEMKHKHGGSADMGMHH
                     FHLLMNHGITMVTEGSNMAMLAEMKMAPGVDKKSLQHGQHMIKEGKDLITRALNGPEM
                     TAMMKQHAQDPLMDYTHQLGESMLKLTDILQKMSMEDMSSPDMMAMHHMHMMINHAVQ
                     MAADGANLIMLGQMGMAGDIDKLTIDHGKAMLSDAKSMVNEMMEGKEMKDMHAKGTTP
                     EKSPMMSMSHKQAEAATKIIDLLAKMPAASSK"
     gene            complement(5100..5495)
                     /locus_tag="VMU21_12880"
     CDS             complement(5100..5495)
                     /locus_tag="VMU21_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78460.1"
                     /translation="MKIKALVLLIAFLPLKAEVAYGKGYEVKKEVGGYIVVMKIDRNP
                     PVAGDNNVSIEVTDAKTLCACQANVAIEYFRPAMPGMPAQRYNVNMTFKRGRHIGKIS
                     LTLPGPWNIAVKITTGDKTWTTNFVLDVE"
     gene            complement(5581..6033)
                     /locus_tag="VMU21_12885"
     CDS             complement(5581..6033)
                     /locus_tag="VMU21_12885"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HUO78461.1"
                     /translation="MSKGIFALPSIKVLILSFILSFLPACSEQPSYPAPLQMGKDVVV
                     DISRIPSEVPEFFTYHYHGKKINFFVVKANNAFLSFLDACVSCYAEKRGYRFDGGYVI
                     CRACNLKYPVSEIERGFGSCYPIRLQGHVQNGKYYISRSLLEGQVDKF"
     gene            complement(6195..7685)
                     /locus_tag="VMU21_12890"
     CDS             complement(6195..7685)
                     /locus_tag="VMU21_12890"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HUO78462.1"
                     /translation="MASIKKCLMAIFLIAILSVALPPEVFAAGAADGVENPKSCKQCG
                     MDRTVFTRSRMLIVYADGTTVGVCSLHCAAAEMRQNRDKQVKSLMVADYVTQELIDAK
                     TATWVVGGNKQGVMTSLPKWAFAKGEDAQKFVKENGGEVTIFAEAMGLAEEEISGEAA
                     TTHTHTHAGHDMGLGAQMLFNPAMGDQIYHTHPAGMWMFNYKFMHMDMNGLRDGTTNV
                     SQENVGFMRGKPYNYMMIPTDMTMDMYMFMAMYGITDRLTVMAMANYLWMNMSMLMDM
                     GPMSMKPVKEEPPMTVNGFSDTELRAMYKINKYLVGSLGLIFPTGSIDNEFTTMGRTY
                     RNPYDMQLSSGTYDLSPALTYNGLSDDAKWNWGAQVQCTWHIGENNNGWSFGDNFKVT
                     SWLQRALGPVTSWLRLAYNDTGKIRGYDPEIAKLLDPNPMKGASMPDADPNNYGGQRL
                     DGAIGLSFQKGPFSIGFEGGIPLYQNLNGLQLKTSWFLNAGLQIMF"
BASE COUNT         2214 a         2178 c         1859 g         2259 t
ORIGIN      
        1 aagcactaca agacctcctc cccagacgga agcagcaaga aggtggagcc agtccattat
       61 ttctgcaaca ctgaaatctc ccttatcaga ggcatgccct gatgcgctcg cagtcatgga
      121 tataagcatt gagaaaccca gcataagtaa caggaatcca cgcgaatcac ggtacggcct
      181 ccccgccttc aacgtaatcg ataaaagtat gagagttgca atgcgaatga gccagaccga
      241 cccaaagtgg gtcttgagaa ttaccgttgg gagtatagga aaagcagcag aaacgggttg
      301 gccgcttatc tctgaagcac gcatcagcaa ctcagcaaca ctgcttgcga ccattacacc
      361 taaacagata ccgaataacc gccacatgga ggcgaggata tttccgtgag agggaaactc
      421 ggcacctgcc aaaggagtaa aaacgaagag acggcagacg agagccccga tgcaaaaggt
      481 caaagaaatg agttggaacc attcaggaac gatgtggaga aaagcattca tctgacgtta
      541 acctccttat ttgattgtaa atgaataatc ccccatggtc ctatgtgtat ccctcgccac
      601 aacattccag aacactcgat aggtacccgg agctagtttg ggcacactaa cctccagaag
      661 tgttgggtcg gacggattca cacgtccgtc cctcttgtct accatcttac cgtcggcgct
      721 gtgaaccata atcgaagaaa aggctggctc cagttcgctg tcaaaccata tgcggactcg
      781 gtcaggagaa gcagatattg tcgcacctac ctttgggtca gagtggtcgg gaaacgcatg
      841 ccccgtcgcg acctccggca ttatgcacag aaaagaaagg gaaaagagtg ccactacagt
      901 cctgattttc atatgctgca aggttttctt tagtgaaaaa ttggtccccc tattcccttg
      961 ggccacaggt catcaacata caaatgaagc aggccgagga tccccacact atgccctgat
     1021 ctggagttga tgggaatctg tgccgcaaca ccaaactcca tatacttgcc gcaccagacg
     1081 actcccgggt tcacggtccc ggtaatcttc cctttacagt ctgcgctcat gcaggtttcc
     1141 atgggaaatt cagtcacaag tatcatccgc ttaaagggag ctccaagccc gatatccttt
     1201 acaaaggatt gaaggtatat aaggctgtac tgtaaggtga atgcccacgt caaagtggtc
     1261 ggattacgct ctatatcggt ttctccggtg tcaggatcca caagcacgtt cttgctcctt
     1321 gtcggaaaat taggaccgat aaccccggtg atcgcaaatg gccggagata tttcacggat
     1381 tccggcaagt cgcctaatcc ttttccaaaa aaaagcgcag gggaaatcgt cgtaaaagaa
     1441 tcggctatct ggtgagcccc ggttccgccg aggtcgaagt cagtgccgac tgaaaggatc
     1501 agctcatggg gcgcattcgt gagaaattgc cattttgcgc ctataccgag attatcccag
     1561 ccgtttgccg aaccatctcc gtttgagccg aggtgcacgt atgcctcatg gaattcgagt
     1621 ccgaagttag gcaggatacg cttggaatag tcgatatcta tttgagtcaa catgttaggc
     1681 tcatctccgg gctgcttaac acggttaata agaatcgaaa actcatctga aatgaacggg
     1741 tcgtcaacct gaaatgtggt aggaaaaaac ctctcacccg caaacccatg ggcccatgaa
     1801 gaggccggta gcaggagaat aatcgccaaa gcgatacaaa gtacacagcc tgatgagaat
     1861 accgttgtta aagcctttgt tctgtaagtc atgcttccct ccttctcata aatttgtgaa
     1921 ttcatacacc atgttcactg atgactatcc gcagttatta taggaccgct cagagagcgc
     1981 aaaaatgcct caagctgtct caattcctcg tcacttaatg tgcggtgacc aagttcatgg
     2041 ctttctgatt gacggtaaaa ttccaggacc tcctttaggg tagtgaattg gcccgcatgc
     2101 atgtaggggg gcctttcggc aacatttctc agggtaggag ttttaaaagc tccgtcatat
     2161 ttatctgttt tagtgtctag gaatcgcagc tcagcacaat cgtgaggccc ggcatcacta
     2221 tatctgccaa gacagttgaa ctcatccgag agaaccttcc tgataccatc tgcacgtcct
     2281 ttatcgtaag gcattcttgc gggagtgggc acacctgtgt tgtgaaagtc gctgttggta
     2341 aagagaggtc cgctatggca attggtgcat tttgccttac cgataaaaag cccgagacca
     2401 tttagttcct cgggggtcag agatatggcc attgtagccg tatctccttt ttgaagggcc
     2461 tcaacgtacc tgtcaaaccg agaaggtccg gggacaatag tccgcacata agcggcaatt
     2521 gctttgccga tgttcgcata aatgcgattg acctcctccc tcttgtcagg tgtcattgac
     2581 acccatgttt taagagcaac agcgtcctca gttgcaggag ttgccactgc atttgtaata
     2641 tccggcaatt tcccgaaaat ttcttcatat tcgttccgat aattcttgct gatcaaataa
     2701 gcatatgctg ttctggtaaa gccatgctct acgttgcttt caatcggccc caaagcttgt
     2761 gaccagaggc tgtcttttct tccatcccag aaaaaccatg tgttatacgc aacgccaagc
     2821 aaaggcatcg ttctccttgt cgttgacccc atgccatgtg ccaggggaag gtcatcgtcg
     2881 aaattcatgt caggtcgatg gcaggtggca caggaaactt ggaggtttcc actaaaccga
     2941 ggatcgaaga agagtctttt gccgagggct ctcgctttag gattatcggc ataggcgttg
     3001 gaaggatctt tcggcagggg aggaagagac cctatccaga gcgagcgaat aaccgatatt
     3061 tcctctttgc tccatgagta agtaccaatc tctgaggccc acgcccagcc aagcgcaata
     3121 ttacacaaag ccatgaacgc tacagccgcg agtattatca cacgcttaaa gctcaacaga
     3181 cagaaatatg ctttcatgtg aacctttctg ctattggacc acaagattaa atgtaacagt
     3241 gtcatccaag tcctgtctct tgactgtaaa cgttatcacc caccatcccg gcatgctgaa
     3301 tttgaggcct tgaacaatat aatccccgtc ctttgtcccc ttgacaacct caggttgagt
     3361 tggaaggccg tggccatgct ccggcatgtc accgttaata ctaattacag catcctttac
     3421 aggacgcccg tctgctgtat taacattcaa tttccaggcg atgattctcc cgacgggaat
     3481 cggtacagtc tcgctcacat aggagacctt gaagagtttc ttcgccgaca tgcgtaccgt
     3541 ggaggtatca acatcagcag gggctgaaga atacgtcgct ttatgttcat ccgctgatga
     3601 tgtttgagag actttaactt caggaaaatc aaagactgcg ctgtcttcca tatccccctt
     3661 cctaatcttt atttttaatt cccagcgccc tcccatgatc agtataatgt tttccacact
     3721 atagagccct ccgccccttt ctctgacaac cggtttttca taaacgccat gccccatttc
     3781 aggcatccag ggagtaaccg ttacttcagc accagccaca tccttgtcgc tcttgtcatg
     3841 cacgatgata tcgagagtgt ttaccccgac tttcagttcc ttttccttga tcaccatttc
     3901 cacagagaac aaccccttct cggtcacttt aaagaggctt tgctcataat gctttgtata
     3961 cgaccctgca ctgggcgggt ctgtgtgatg ggcaagccct acagaaggga ttaacaacgc
     4021 gagaataaac acgattacag ctctcatggt cgcaacctcg attcaaagaa ataaagagat
     4081 gttattatct cccgttcaat gtttattgat atgtgagttc cttctaggga aacaaggcag
     4141 ggcttagaag ccccgccctg ttccctcaat attattattt agagctcgcc gcaggcattt
     4201 ttgcaagcaa atcaatgatt ttcgttgctg cctcagcctg cttgtgggac atgctcatca
     4261 ttggtgattt ctcaggcgtt gtaccctttg cgtgcatgtc cttcatttcc ttgccctcca
     4321 tcatttcatt gaccatagac ttcgcatcac tcaacattgc tttaccatgg tcgatggtaa
     4381 gtttgtcgat atctcctgcc atgcccatct ggccgagcat tataagattg gcaccatcag
     4441 cagccatctg aactgcatgg ttaatcatca tatgcatgtg atgcattgcc atcatatcgg
     4501 gagatgacat gtcctccatg gacatcttct gaaggatatc cgtcagcttg agcattgact
     4561 cacccaactg atgagtatag tccatgagag ggtcctgggc gtgttgtttc atcatggctg
     4621 tcatctcagg accattcaga gcacgagtaa taaggtcctt gccttccttg atcatgtgct
     4681 gcccgtgctg cagggatttt ttgtcaactc ctggagccat tttcatttcc gcaagcattg
     4741 ccatattgga gccctcagta accatcgtaa tgccatgatt cataagcaag tggaaatggt
     4801 gcatgcccat gtcagcactt ccgccatgct tatgcttcat ctcctcggca tacaccagac
     4861 cggaaaccac taaaaaaaca gcgaccacca cagtgagcaa agataccaac gtaaccttac
     4921 ttttcatcgc aatacctcct gttaccttaa aattaatgag acgtcttacg acaatacctt
     4981 tttcctttta ccacatgaat taacaaagaa acaccgcacc cctgtatttc tctgtgaaaa
     5041 cctcctttcc ccaatagtca cattgtctta acggattgca cttcataccc aatgcatcat
     5101 tattcgacat ccaggacgaa gtttgttgtc caggtcttat ctcctgttgt tattttcact
     5161 gctatattcc agggaccggg caaagttaag cttatctttc ctatgtgtct tcctctcttg
     5221 aaagtcatgt taacgttata gcgttgagca ggcattcccg gcatcgcagg cctgaaatat
     5281 tcgattgcca cgttcgcctg acacgcgcat aacgtttttg catcggtgac ctctatactc
     5341 acattgttgt cacccgcaac aggtgggttc cgatcaatct tcataaccac gatgtatccg
     5401 cccacttcct tttttacttc atatcctttt ccataggcga cctcagcctt caaaggaaga
     5461 aacgcgatca gcaaaacaag tgccttaatt ttcataattc agtctctcgt cctttcactt
     5521 gtcacttccc gtgataaccg caaatcgcga gaaagtcata gatgcccaat ggctttttct
     5581 tcaaaacttg tccacctgtc cttctaagag cgaacggctg atatagtatt ttccgttctg
     5641 cacgtgtcct tgaagcctta tgggataaca actcccgaac cctctttcta tctcagacac
     5701 cggatatttc aaattgcacg ctctgcaaat cacatatcct ccgtcaaagc gatatcctct
     5761 tttttctgca taacaactca cacatgcgtc aaggaacgaa agaaatgcat tgttagcctt
     5821 aaccacaaag aagttgatct tcttcccgtg atagtgatat gtaaaaaact cagggacctc
     5881 agagggaatc ctgcttatgt caaccacaac atccttgccc atctggagcg gtgcgggata
     5941 ggagggctgc tcagaacatg cagggagaaa tgacaagata aaagacagga tgagcacctt
     6001 gatgctaggt agcgcgaata ttcctttgct catttgttaa ccatatctaa gagccacaag
     6061 agacaccagc aagaagcttc atttctttct gccttcaaag gtcttatgac ttgataaagc
     6121 atatcataga atcctaaagg cctcaattaa agcacgataa cccatccata acttacccag
     6181 tggcaaatcg gagtttaaaa cattatctgc aatcctgcat taagaaacca ggaagttttc
     6241 agctgcaaac catttaggtt ctgatagaga ggtataccgc cttcaaaacc tatgctgaag
     6301 ggtcctttct gaaaacttag ccctatagcg ccatcgagtc gttgtcctcc gtagttgttg
     6361 gggtctgcgt caggcatcga tgcccctttc ataggattag gatcgaggag ctttgcaatc
     6421 tctgggtcat accctctaat ttttccggtg tcgttatagg caagtctcag ccaggaagtc
     6481 accgggccta aggcacgctg cagccaacta gttactttga aattatcgcc gaagctccag
     6541 ccattgttat tctccccgat atgccaggta cactgaacct gggcccccca attccacttt
     6601 gcatcgtcac tcaggccatt atatgtgagc gccggactta gatcatatgt gcctgagcta
     6661 agctgcatgt cgtaagggtt gcgatatgtc cttcccatcg tggtaaactc gttgtcgatg
     6721 ctgccggtcg gaaagatcag accaaggctg ccgactaaat acttgttgat cttgtacatt
     6781 gccctcaatt cggtgtcact aaaaccgttg accgtcatag ggggctcctc cttgacaggt
     6841 ttcatcgaca tcggtcccat gtccatcagc atactcatgt tcatccacag gtagttcgcc
     6901 atggccatca ctgtaagccg gtcggtgatg ccgtacatcg ccatgaacat atacatatcc
     6961 atagtcatat cggtgggaat catcatataa ttataaggct tgcctcgcat gaagccgaca
     7021 ttctcctggc tgacattcgt ggtaccgtcg cggagcccat tcatatccat gtgcatgaat
     7081 ttatagttga acatccacat tccggcaggg tgcgtatgat atatctggtc acccatggca
     7141 gggttaaaca gcatctgggc gccgagcccc atatcatgtc ccgcatgtgt gtgcgtgtgc
     7201 gtcgtcgcag cctctccgct gatttcctct tcggccaatc ccattgcttc cgcgaagatc
     7261 gttacttccc ctccattttc tttcacaaat ttctgtgcat cctctccctt ggcaaacgcc
     7321 cattttggca gggaggtcat gacgccctgc ttgttgcctc cgaccaccca ggttgcagtt
     7381 ttcgcatcta tcagctcctg ggttacgtaa tcggcaacca tgagcgactt gacctgtttg
     7441 tccctatttt gtctcatctc cgcagctgcg cagtggagac tgcagacccc tacagtggtc
     7501 ccatcggcat agacgatgag catcctgctc cgggtaaaca cggtccgatc catcccacat
     7561 tgcttgcaac ttttgggatt ttcaacccca tccgctgcgc cggcggcaaa gacttccggt
     7621 ggaagtgcca cactaagaat cgcgatgagg aagattgcca tcaagcattt ctttatggaa
     7681 gccaatatgt ttcccctcct gtttgcagaa atcttacttt cctcctcaat agttttttca
     7741 gcgtactgaa agctcgccgt ttctaagagc atccgtaaaa aggtggctcc actccggatt
     7801 tatttttcaa taatttccga agcggagccg agtttaaagg ggaggaaagt ctgaagaaga
     7861 actctcctca tacaggctgc acgccagtat ttctgaaaaa actggctcga agacttcggt
     7921 actgttgatt aagacttggg agggtgttct aagtcagagg cgattaaaga tggcttgctt
     7981 gaaggatcaa tgattctggt atatccgcaa agccccaaag ggcataattt acagagggat
     8041 tcctgaagag cggggatgtc ggcgttaacg gacacggcac tgccggatgc gtgacatacg
     8101 tcaagagtta cgatatacga atgatcgtct aaaggagaaa ttgaaataga caaaggggaa
     8161 agaattgaaa aggaaaggac aatcaaaatc agaatggatt gtatcttcat acgaatagag
     8221 tttaaatata aaaatcatcc ctgtcaacat aaaattacag ttttaatttg ttggttatcc
     8281 ggccttcatt gcttattcat ctcccgccct tgttattgca cagcaggagg aaatcgatag
     8341 gtatgaaaac ttgaaatcgg tacggtgtac agtcatctat gcacagacct ccttgaacat
     8401 tgtcctttga gtaagcatcg cgaagataac acgaacgagc ttatgcgccg ttgcaaagac
     8461 cgctttcttg aacggttgcc cctcgcttct ctttttcatg aagtaggccg
//
LOCUS       DATKOU010000270        11348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3454597, whole genome shotgun sequence.
ACCESSION   DATKOU010000270 DATKOU010000000
VERSION     DATKOU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454597"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VMU21_12895"
     CDS             complement(<1..359)
                     /locus_tag="VMU21_12895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUO78463.1"
                     /translation="MTVSVVVPVYNEEDNVETLHKRLRASLGRLGQEYEIIYVDDGST
                     DKTLSLLEQMQAHDPAVIVLSLRRNFGQTAAFAAGFDFARGDVVVTMDGDLQNDPDDI
                     PKLLELIKDNDLVSGWR"
     gene            complement(359..2383)
                     /locus_tag="VMU21_12900"
     CDS             complement(359..2383)
                     /locus_tag="VMU21_12900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUO78464.1"
                     /translation="MPEWIQQLDTALFFFLNKDCQNWLFDLIMPVFSHRMNIIVLPFL
                     LLFFIKEKKGAMVIFAISFFSILLADGTSHALKEMIERARPCNSFQNVHLLVGCSQSF
                     SLPSGHATNSFAFAVPFLFMTRDRLRYVFLGIAVFVSLSRVFVGVHYPSDVITGAVVG
                     ILSSTAVVYLYRWAEKRFREKPHTTVMCIFLLTLSIFRIYYILYGPLDLSPDEAHYWE
                     WSRRLDLSYYSKGPMIAYLIALSTSLFGNNVLGVRFLAVVFSVLSSIFLYKLGKEMYH
                     ETVGTASALLFQLIPLYSAFGVIFTIDSPFIFFWIVSLYLFWKATGQDTSGHSYRGYW
                     LLLGVSLGLGLLTKYTMAFFYLCAFLFLILSSHKRVLLKAISPYLALVISILIFSPVL
                     IWNAGHDWVTFRHTAGQAHLADGLQISFKSLTEFMGSQVGVVTPILFVLIVIALWRIK
                     GKVRRDFLLWFSLPVILFFVVKSVQGKVQANWAMTGYITGLIAFSEVYMSRWKEHGDL
                     LKKIIIAGIVLSFMVAAIAHYPSKFHVPVTLDPSSRLRGWKELGRQVSDLHDEMRKGG
                     RVFILSDSYQISSELAFYGKGHPITYCVNLGRRMNQYDLWPDFYNLITSDAIFVTIGG
                     AELHPGIRDAFHHYEKRFLRVYDKDRLLREYSVFLCHGFKGMKREAAGSY"
     gene            complement(2634..3896)
                     /locus_tag="VMU21_12905"
     CDS             complement(2634..3896)
                     /locus_tag="VMU21_12905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUO78465.1"
                     /translation="MASPQTVSRNPWFFVPTLYFGEGLPYVLINTVSLILYKKMGVDN
                     TDIAFWTSWLYLPWVIKMFWAPILDLTWTKRTWVLAAQGAIALNIGFIAYAVGKPDFF
                     FLSLGAFMIGAFISATYDTATDGYYMLALAEKEQAFFTGLRSGFYRLAMIFGSGLLVY
                     FAGGVETATGSIRASWMAVLGISSGIFIIFWLYHYAVLPYAPTDHPRSAADAIQKPTF
                     KQVFSAYFRQEKVRVLVAFILFYRLGEAMLLKLVTPFLLDRRTDGGLGLATSEVGLVY
                     GTVGIIALVLGGVLGGWLISQYGLRRCTWPLVITMHMPDLFFVYMAWAQPSLAMVYPL
                     VALEQFGYGMGFAVFTVYLMYSAKGLYKTSHYAISTGIMAIGMMLPGFISGWVQQKIG
                     YPLFFVIVCLLTIPGMLTIPLLPKREKT"
     gene            4258..5127
                     /locus_tag="VMU21_12910"
     CDS             4258..5127
                     /locus_tag="VMU21_12910"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUO78466.1"
                     /translation="MAYRELSAKQTTRKRTSDRRIAIEHKMRKAFVGGKGQVKKSKRI
                     KIQAAMLMCYVLLSILLASADDFEDGTKAYRGGDYKTAYQLFLKAAEQGNARAKTYLR
                     MMCANGQGVEKGCKESAKSDRLVTKKGQAGAQPNLGALYDKEQGVPQDEKEAVKSYRL
                     AAEQGDARAQVALGVRYETGDGVQRDYKEALKWYHLAADQGYALAQTYLGVLYKTGER
                     VPQDYREAAKWFRLATDQGDALAKCNLSALYASGRGVSRNMSIAKQLAKEGINAGEKL
                     CQEVWKKYNLANY"
     gene            complement(5378..6097)
                     /locus_tag="VMU21_12915"
     CDS             complement(5378..6097)
                     /locus_tag="VMU21_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUO78467.1"
                     /translation="MLIEGPHLIEMALASRTGIKRVFFTDSSYSKYPGLFKQLSSNDA
                     ELFETTEHVLGHLSDTETPQGIAAIASFNFLDLEELSLGLNPLAVICDSIQDPGNLGT
                     IIRTADAAGADAVIILPGTCDPLLPKVVRATAGSVFTIPLVFVESRVLLQWLKEKAIT
                     LFVTDVNASRSIYETDLRKPLAFALGNEAKGVNESLRREGEVFVKIPILGRAESLNVA
                     TSAAICLYEAVRQRGALASVW"
     gene            6290..6913
                     /gene="nth"
                     /locus_tag="VMU21_12920"
     CDS             6290..6913
                     /gene="nth"
                     /locus_tag="VMU21_12920"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HUO78468.1"
                     /translation="MLKKQVKSLRIPWLEDMASTGRDPFKILISCILSLRTQDRTTGL
                     ASERLFKLASTPEDMSGLSPRQIEKAIYPVGFYRVKAKTIKTLSREIFLRYNSRVPDE
                     IDELLTLKGVGRKTANLVVTLGYDKPGICVDTHVHRISNRWGLVRTKTPHETEFALRV
                     ILPQKYWKEINGLLVAFGQSICKPLSPLCSTCALHRYCPKLGVTRHR"
     gene            complement(6976..7851)
                     /gene="dapA"
                     /locus_tag="VMU21_12925"
     CDS             complement(6976..7851)
                     /gene="dapA"
                     /locus_tag="VMU21_12925"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HUO78469.1"
                     /translation="MLEGSIVAIVTPFKGGKVDEKALSELVEWHVSSGTNAIVPCGTT
                     GESATLEYEEHYRVIEIVIKTANKRIPVIAGTGANATDETIMITKKAKKLGADAALLV
                     APYYNKPTQEGLYRHYKSVAEAVDIPIVLYNVPGRTAVNILPSTVARLLEIENIVAIK
                     EATGDMKQVSEVIRLCGDRLTVLSGDDFTTFPLLALGGKGVISVTANIAPREVSEMCR
                     LWKEKKYEKARALHYKLEPLNSTMFIETNPIPAKTAVAMMGKIREEFRLPLCEMSEAN
                     REKLKKTIKDFGLIK"
     gene            complement(8015..8902)
                     /gene="dapF"
                     /locus_tag="VMU21_12930"
     CDS             complement(8015..8902)
                     /gene="dapF"
                     /locus_tag="VMU21_12930"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HUO78470.1"
                     /translation="MKIEFTKMHGLGNDFILIDCRETKIPNLSKLSERLCDRRFGIGA
                     DQLLTLYPSRVADFRMGIFNADGSEVEMCGNGIRCLAKYIWDRGLSEKRAIQVETLAG
                     IIRPERVNGLIRVDMGEPILEPEKIPVDIRQRSAVSSQQLAGQPRAKNSKFVMDYPLQ
                     IGDREFRVTCVSMGNPHAVIVTDSLSEFPVAYYGPAIEHHPLFPKRTNVEFIEVLNRS
                     EIRMKVWERGAGETMACGTGACAAAVAAHLQGLTERSVTTHLGGGDLFIDWDKDAHVY
                     MTGPAAEVFTGTINLDSLG"
     gene            complement(8911..10164)
                     /gene="lysA"
                     /locus_tag="VMU21_12935"
     CDS             complement(8911..10164)
                     /gene="lysA"
                     /locus_tag="VMU21_12935"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HUO78471.1"
                     /translation="MHFFRYRNGELYAEDVPVREIAEAHGTPLYIYSHKTLLRHFRAY
                     ANAFKGIPHIICFALKANSNSAILRLFAERGGGADVVSGGEVFRALRAGIPSRKIVYA
                     GVGKTEEEIAYALKSKILMFNVESEQELMEIDRVAGTLKVKAPISLRVNPDIDPETHP
                     YISTGLRKHKFGIPIEDAFEYYKLAKHLRNTHIVGIHKHIGSQITKVSPFVEALKKTL
                     LLVDELNGHGVHLQYLDIGGGLGITYKDEEAPVPQTLARNLIPMLKGRRLTLIMEPGR
                     SIVGNAGILVTRVLYLKKGEEKDFVIVDAGMNDLIRPSFYDAYHQVLPVKKNRRRNML
                     ADVVGPICESGDFLAKEREIPVVMQGEYLAVMSAGAYGFTMSSNYNSRPRVAEVLVKE
                     QDFALVRRRETYKDLISGEIVPSFI"
     gene            10290..10694
                     /locus_tag="VMU21_12940"
     CDS             10290..10694
                     /locus_tag="VMU21_12940"
                     /inference="COORDINATES: protein motif:HMM:NF018621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HUO78472.1"
                     /translation="MPRLRGHHLICLHFFNGEGYNPEFVGNLKDVLEKTKSGDVEISP
                     GADDICEGCPYLKQFRCRYTEDADEGIREMDKTALELLKEEEGAKVKWHEVRKRIPEL
                     FPSWYRIYCTTCGWKRACERDASYRQLRASLS"
     gene            10701..11078
                     /locus_tag="VMU21_12945"
     CDS             10701..11078
                     /locus_tag="VMU21_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HUO78473.1"
                     /translation="MKAKKNIIIVGDRVLIEQDEKTDKTTSGLYLPPTVKEKERVLGG
                     YIIRVGPGYPVHDPNVVIEEPWSSHKRMDLRYLPLQASEGDYAIFLKEAAVEIEFEGK
                     KYLIVPHSAILALVRTEITEGES"
BASE COUNT         3007 a         2781 c         2783 g         2777 t
ORIGIN      
        1 tttctccagc cgctcacgag gtcattgtcc tttataagct caaggagctt ggggatatcg
       61 tcagggtcgt tctgcaaatc accatccatg gtaaccacca catcacccct ggcaaagtca
      121 aaacccgcgg caaaggcagc cgtttgtccg aaattcctcc tcaggctgag cacgattacc
      181 gcggggtcgt gtgcctgcat ctgttcaagg agggaaaggg tcttgtctgt gctcccatcg
      241 tccacataga tgatctcata ttcctgacca agacggccaa ggctcgccct gagccttttg
      301 tggagggtct caacattatc ctcctcatta tagaccggaa ccacaaccga tacggtcatt
      361 aataactccc tgccgcctct cgcttcatcc ctttaaaccc atggcataaa aaaacggaat
      421 attccctcaa gagtctgtcc ttatcataaa ccctcagaaa acgcttttcg taatggtgaa
      481 aggcgtctct gatccccggg tgcagttctg caccgcctat tgtaacaaaa atggcgtcgg
      541 aagtgataag gttgtaaaaa tcaggccaga gatcatattg gttcattctc ctcccgagat
      601 tcacacaata ggtaatggga tgcccctttc catagaacgc aagttcactg gatatctggt
      661 aactgtcgga aaggataaac acccttcccc ccttccgcat ctcgtcatga agatcactca
      721 cctgccggcc gagttctttc caccctctga gtctcgatga cgggtcaagc gttacaggaa
      781 catgaaattt cgacgggtaa tgggcaatgg cggccaccat gaaggacagg acaatgccag
      841 caataatgat ttttttaaga aggtctccgt gttccttcca tctgctcatg tatacctctg
      901 aaaatgcaat aagaccggtg atatatccgg tcatcgccca gtttgcctgg acctttccct
      961 ggacgctctt cacgacaaaa aacaaaatta ccgggagaga gaaccagagg aggaagtccc
     1021 ttctcacttt ccctttaatt ctccataggg caataacgat aagcacgaag agaatgggcg
     1081 ttactactcc cacttgagaa cccataaatt cagtgaggct tttgaatgat atctgcaaac
     1141 cgtcagccag atgcgcctga cctgcggtat gtctgaacgt cacccagtcg tgccctgcgt
     1201 tccagatgag tacgggtgag aagatgagta tgctgatcac tagggcgaga tagggagaga
     1261 tagccttaag gaggaccctt ttatgcgaag agagaataag aaaaaggaat gcacagagat
     1321 agaaaaaggc cattgtatat tttgtgagca ggcctaatcc cagggagaca ccgaggagca
     1381 gccaatatcc ccgatatgaa tgtccggagg tgtcctgacc cgttgccttc cagaaaaggt
     1441 acagtgacac gatccagaaa aaaatgaacg gcgaatcaat ggtgaagatc actccaaagg
     1501 cggaataaag gggtatgagc tgaaaaagga gggcggaggc cgttcccaca gtttcatgat
     1561 acatctcctt gcccagtttg tagagaaata tgctgctcag gacggaaaag acgaccgcaa
     1621 ggaatctcac gccaagcaca ttgttcccga aaagagatgt ggagagagcg ataagatacg
     1681 cgatcattgg tcctttggag taatagctta aatcgagcct ccgcgaccat tcccaatagt
     1741 gggcctcatc gggactgaga tcgagggggc cgtagagaat gtaatagatg cggaaaatac
     1801 tgagagtgag gagaaagata cacatgacag ttgtatgagg tttctctcga aatcgtttct
     1861 ctgcccacct atagagatac accacggccg tggatgagag gatgcccacc acggctcctg
     1921 taataacatc agaggggtaa tgcaccccca cgaataccct cgaaagagat acgaacaccg
     1981 ctatccccag aaagacatat ctaaggcggt ctcgtgtcat gaaaaggaaa gggaccgcaa
     2041 aggcaaagga attcgtggcg tggcctgagg ggagggagaa ggattgtgaa cagcctacca
     2101 ggagatgcac attctggaag ctattgcatg gcctcgccct ttcaatcatc tcctttaagg
     2161 catggcttgt tccgtccgca agcaggatcg aaaaaaaaga tatcgcaaag atcaccatcg
     2221 cccccttctt ttcctttata aagaacagga gaaggaaggg aagcacaata atattcatcc
     2281 tgtgcgaaaa cacgggcatg ataaggtcga acaaccagtt ctggcaatct ttgttcagga
     2341 aaaaaaagag ggcagtatcc agctgttgta tccattcagg catgctgtat aatatcacag
     2401 tgaatgtttc cctaactcac tttattcatt gatttctttg cttcctgaga ggcatccgtc
     2461 tcaccttgca agacaatagc tcttctcgac tgtactgggc tttgcatacc taacaatacc
     2521 ctgtgtttat gtatcgttaa ggtctgcacc tgcagactgg cacataaccc ttccgcctca
     2581 ggcggattgc cgtccgagcc agataccttt cacgaattga tgaattacta aggctaagtt
     2641 ttttcccttt ttggaaggag cgggatagtc agcatgccgg ggatagtgag aaggcagaca
     2701 attacgaaga agaggggata cccgatcttt tgttgaaccc agccggagat gaacccgggg
     2761 agcatcatac cgatggccat gatacccgtg gagatggcat aatgggaggt cttgtacagg
     2821 cctttggcgg agtacatgag atagaccgta aagaccgcga atcccatgcc gtaaccgaac
     2881 tgctccagtg caaccagcgg atagaccatc gcaagggagg gctgcgccca tgccatgtac
     2941 acgaagaaca agtctggcat gtgcatggtg atcacgagcg gccaggtgca acggcggagt
     3001 ccatactgcg agatgagcca tcccccgagc acgcctccca gcaccagcgc gataatgccc
     3061 acagtaccat agaccaggcc gacctctgat gtagcaagcc cgagccctcc atcggttcgt
     3121 ctgtccagca gaaacggcgt gaccagcttg agcagcatgg cctcgcctaa acgatagaac
     3181 agtataaaag ccacgagcac tctcaccttt tcctgccgga aataggcgga aaaaacctgt
     3241 ttgaaagtgg gtttttgaat agcatctgcc gctgaacggg gatggtcagt cggcgcgtac
     3301 ggaaggactg cataatgata gagccaaaag atgataaaga ttccgctact gatacccagg
     3361 accgccatcc agctggcacg gatactgccc gttgctgttt cgacacctcc tgcgaaatag
     3421 accagtaatc ccgagccgaa aatcattgcc agtcgataaa agcctgatcg aagcccggtg
     3481 aaaaaggcct gctccttttc cgcaagggcc agcatatagt agccgtcagt ggcagtatca
     3541 taggttgcag agatgaatgc gccgatcatg aacgctccaa gggataggaa aaaaaagtcc
     3601 ggtttgccga ccgcataggc aatgaagcca atgttcaacg cgatggctcc ttgggccgca
     3661 agcacccagg tgcgcttggt ccatgtgaga tcgaggatcg gtgcccagaa catctttata
     3721 acccagggga ggtacagcca gcttgtccag aaggcaatgt ccgtgttgtc cactcccatc
     3781 ttcttataga gtatgagtga aactgtgttg atgaggacgt agggcaggcc ttcaccaaag
     3841 tagagcgtgg gaacgaagaa ccatggattg cgggatacgg tttgtggtga cgccatgcag
     3901 ggaaggatat cagattacga aggaatcatc cacttgaaaa ataaaacatt tatccctata
     3961 ttccttccgc gcggattacg gaagcaggtg ttgctcagag actccgtttt cattaggtaa
     4021 ggccttcgcc ccatactcca catggctatt gtatgcagtc caccgacgaa ccaacgtggt
     4081 tcaagaagac gcaaccatga agattattga tagaagcgat tcgctgttat caacttggaa
     4141 cgtatacata ttgcagatat tgagacgagt atgaaaagaa ggttatgata taagaaacgg
     4201 ttcataaaat atgattcgat ttctacttat tgcatccgag agaggaaaag tctattggtg
     4261 gcctatagag agctgtccgc aaagcaaaca accagaaaaa gaacaagtga tagacgaatc
     4321 gcgattgaac ataaaatgag aaaggctttt gtggggggaa aaggacaagt taaaaagtca
     4381 aaaaggataa agattcaagc cgcgatgtta atgtgttatg tccttctaag catcttgttg
     4441 gcctctgcag atgattttga ggatggaaca aaagcatata ggggcgggga ctacaaaact
     4501 gcataccagt tatttttgaa agccgctgag cagggaaatg ctagggcaaa aacctatctt
     4561 cgcatgatgt gcgctaatgg acagggagtg gagaagggtt gcaaggaatc tgcaaaaagt
     4621 gaccgtcttg tcactaaaaa gggacaggct ggtgcacaac ctaacctcgg tgctctgtac
     4681 gataaagaac aaggagtacc gcaagacgaa aaagaagctg tgaagtcata ccgtcttgcc
     4741 gctgagcagg gagatgctag ggcacaagtc gctcttggtg ttaggtacga gacaggcgac
     4801 ggtgtgcagc gggattacaa agaagctctg aagtggtacc atctagctgc tgaccagggg
     4861 tatgctttgg cacaaaccta tcttggtgtt ttgtacaaga ctggcgagag agttccacag
     4921 gactataggg aagctgcaaa gtggtttcgt cttgccactg accagggaga tgctttggcg
     4981 aaatgtaatc tttcagctct ttacgccagc ggtaggggag tgagcagaaa tatgtctatc
     5041 gcaaaacaac ttgccaaaga aggcataaat gcaggagaaa agttatgcca ggaagtctgg
     5101 aaaaagtata acctggcgaa ttattagtca aacaatcacg aaatagttct aagtttttgt
     5161 gggtgccacg gcacgtcccc tatagatttc cgtttgcagg gtttagttat cagaactgca
     5221 ttaaaggtgt tgactccagg aaggaaatct cgccgcatgt tttgggctga agccgattat
     5281 cccccggtgg ggtggagggc actccccaag actgtacaga aaaaactgga cgattatctg
     5341 tgtcacaact taccatttac tattcacttt tcacacttca ccataccgaa gcgagtgctc
     5401 ccctctgcct gaccgcctca taaagacaga tggccgcgga tgttgcaaca ttgaggcttt
     5461 ctgccctgcc cagtattggt atcttcacga agacttctcc ttccctcctc agagattcat
     5521 ttactccctt cgcctcattc cccagtgcaa aggccagtgg ctttcgaagg tctgtctcat
     5581 aaatggacct cgatgcattg acatcagtta caaaaagggt aatggccttt tccttcagcc
     5641 actgaagcaa caccctggat tctacaaaga caagaggaat ggtgaatacg cttcccgccg
     5701 tggcacgtac cacctttggc agtaaggggt cacatgtccc agggagaata atgaccgcat
     5761 cagctcccgc agcatcagcg gtcctgatta tggtgccaag attacccgga tcctggatgc
     5821 tgtcacagat aaccgcaagg ggatttagac ccaatgagag ttcttcgaga tcaaggaaat
     5881 taaaggatgc gatagctgca ataccctgcg gtgtctcggt atcggagagg tgtccaagca
     5941 cgtgctcggt cgtttcgaag agttctgcat cattgctcga cagttgcttg aacaaccccg
     6001 gatatttcga gtaagaactg tctgtaaaaa agaccctttt tatacctgtg cgggaagcaa
     6061 gcgccatctc gatgagatgc ggtccttcta taagcatgtc gtgacgcctg ctgattttcc
     6121 tatcccttat ctttagggcc tctttgatgt gcgggttcga cgggcttgtt atctttttgt
     6181 atctcataat taagatagta agtgtcagtt cattatacat aattgcggat cgatcggtta
     6241 taatgcccac tcacatatga agcatttccc tatttatagg atatggccct tgctgaaaaa
     6301 acaggtgaaa agcctccgga taccatggct ggaagatatg gcatcaaccg gaagggaccc
     6361 cttcaaaata ctcatctcct gcatcttaag tttaagaacg caggacagga caacagggct
     6421 cgcttcagaa cggctcttta agttggcatc aacaccagaa gacatgtcag ggctttcacc
     6481 ccggcagata gaaaaggcca tatatcctgt tggcttttac cgagtaaaag caaagacgat
     6541 caaaactctg agcagagaaa tcttcctgag gtacaattcc cgtgtgccgg atgagattga
     6601 cgaattgctg acactgaagg gagtcgggag aaaaaccgcc aaccttgtgg taacccttgg
     6661 atatgacaaa cccggcatat gtgtggacac ccatgttcac aggatttcaa accgatgggg
     6721 gctggtaagg acaaagactc cccatgagac cgagtttgca ctcagggtaa tacttcccca
     6781 gaagtactgg aaagagatca atggattatt ggttgccttt ggccagagca tatgcaaacc
     6841 cctctctccc ctctgcagca cctgtgccct gcatagatat tgtccgaagc ttggggtgac
     6901 gcgacatcgt taacacgaat aagaagccct ctctgctgag agggcctgga actcgactat
     6961 cccgttttca ctctcctatt taatcaatcc gaaatccttt atggttttct tgagcttctc
     7021 cctgttcgcc tcagacattt cacagagggg aagacggaat tcctctcgta tcttccccat
     7081 catagcaacg gctgtttttg ccggaatggg atttgtttct atgaacatgg tggagttgag
     7141 gggctccagc ttgtaatgaa gggcccttgc cttttcatat ttcttctcct tccagagcct
     7201 gcacatctcg gagacctcgc ggggcgctat attcgccgtg accgaaatga cccccttgcc
     7261 tcccagtgcg agcaggggga aggtggtaaa gtcatcaccg gaaaggacgg tcagtctgtc
     7321 accgcatagt ctgatgacct cgctcacctg cttcatatcg cctgttgcct ctttaatggc
     7381 gacgatgttc tcgatctcca aaagtcttgc cacagtggaa gggagaatat tgaccgcggt
     7441 cctgccaggg acattataaa ggacaatggg gatgtccaca gcctctgcca cgcttttgta
     7501 gtgccggtaa agaccttcct gggttggctt gttgtaatag ggggctacga ggagtgccgc
     7561 atccgcaccg agtttctttg ccttcttggt gatcattatg gtctcatccg ttgcatttgc
     7621 tccggtcccc gcgattacgg gaatacgttt atttgccgtc ttgatcacga tctcgatcac
     7681 cctgtagtgc tcctcgtatt caagggtcgc ggattcccct gtggtaccgc agggcacaat
     7741 ggcatttgtg ccgctggata catgccactc taccagttca gacaatgcct tctcatcaac
     7801 ctttccccct ttgaatgggg tcacaattgc tactatagaa ccttccagca tagtgtcctc
     7861 ctcactgaat atagaaaatt tctcggttgc ctatttaagg aatgagcgac cgtccgcgct
     7921 ccttccctca ccatgttcat ttttccggca cattactctt acacggtatc tcttcttatc
     7981 tgcccccccc gtgatgtgta tcacccctcc tccctcatcc caatgagtca aggttgatcg
     8041 tacctgtgaa tacctcagcc gcgggtcccg tcatgtacac atgggcatcc ttgtcccagt
     8101 caataaacaa atcaccaccg cccagatgag tggtgacgct cctctctgtg aggccctgga
     8161 ggtgagcggc aaccgcggcc gcgcatgccc ctgtaccgca tgccatggtc tcccctgcac
     8221 ccctttccca cactttcatt cgtatctctg agcgattcag gacctcaata aattccacat
     8281 ttgtcctctt cgggaagaga ggatgatgct ctattgcagg cccgtagtat gccaccggga
     8341 attccgatag actatcagta acaatcaccg catgggggtt tcccatagag acacaggtga
     8401 ccctgaattc cctgtcacca atctgaagag gataatccat aacgaacttt gagtttttgg
     8461 ctcttggttg tcccgctaac tgttgactgc tgactgctga ccgttgacga atatccaccg
     8521 gtatcttttc cggctcaaga atcggctcgc ccatatccac cctgataagg ccatttaccc
     8581 tttctggtct tataatgccc gcgagggttt ctacctggat ggccctcttt tcagaaaggc
     8641 ccctgtccca gatatatttt gcgagacatc tgatgccgtt gccgcacatc tcaacctcgc
     8701 ttccatctgc attgaaaata cccatcctga aatcggcgac gcgtgaagga taaagggtga
     8761 ggagttggtc agcccctatg ccaaaccttc tgtcgcagag tctctcggag agctttgaga
     8821 gattgggaat tttagtttcc ctgcaatcta tgaggatgaa gtcattcccc agcccgtgca
     8881 ttttggtaaa ctctattttc atcttgtcct tcaaataaac gaggggacga tctcgccgct
     8941 gatgaggtcc ttgtaggtct ctctcctgcg cacgagagca aaatcctgtt cttttaccag
     9001 tacctctgca accctcggcc tgctgttgta atttgaactc atggtaaatc catatgcacc
     9061 ggcggacatc accgcgaggt attcaccttg cattacaacg gggatctccc tctcctttgc
     9121 gagaaaatcg ccggactcgc atatggggcc caccacatca gcgagcatgt tccgtcttcg
     9181 gttttttttc accggcagta cctgatgata ggcatcgtag aaggagggcc ttataagatc
     9241 attcatcccc gcatccacta tcacgaaatc cttttcctcc ccttttttca ggtacaagac
     9301 tctcgtaacc agtatccccg cgttgcccac aatagacctg ccgggttcca taatgagggt
     9361 gagccttcgc cccttaagca tcggtataag gttccttgca agggtctggg gaacaggggc
     9421 ttcttcgtct ttataggtaa tgcccagccc acctcctata tcaagatact gtaaatgtac
     9481 accatggcca ttgagttcat ccacaaggag aagcgtcttt ttcagcgcct ccacaaaggg
     9541 ggaaaccttg gtgatttgag aaccaatatg cttgtgaatg cccacgatgt gtgtgttcct
     9601 gagatgtttt gcaagtttat agtactcaaa ggcatcctca atagggatac caaatttgtg
     9661 ttttctcagg cccgtcgata tatatggatg cgtctcagga tcgatgtcag gattcaccct
     9721 gagcgaaatt ggcgccttaa ccttcaaggt ccctgcaacc ctgtcaattt ccatgagttc
     9781 ctgttctgac tcgacattga acatcagaat ctttgacttc agggcatagg cgatctcctc
     9841 ttctgttttc ccgacccccg catacacgat cttgcgtgaa ggaatgcctg ctcttaaggc
     9901 cctgaacact tcccctcctg agaccacatc agcacctcca ccgcgttctg caaacagtct
     9961 cagtattgcc gaattggaat ttgccttgag tgcaaagcag atgatatggg ggatgccctt
    10021 aaaggcatta gcgtaagccc tgaaatgcct gaggagagtc ttatgactgt aaatataaag
    10081 gggcgttccg tgtgcctcgg cgatctccct tacggggacg tcttccgcgt atagttcacc
    10141 gtttcggtat ctaaaaaagt gcacgtgggt ctccttgatt tatctctatc ttttcccctt
    10201 agatcaaggt attatataat aaaaaatcag tgaggacaac cgcacaaaaa atcattcgtc
    10261 ttctcctcac cctttatgcg tgaggtgcga tgccgcgtct gagaggacat catctcatct
    10321 gcctccattt ttttaatggc gaggggtata atccagagtt tgttgggaat ctcaaagacg
    10381 tgctggagaa aacaaaatca ggggacgtgg aaatatctcc gggagcagat gatatctgtg
    10441 aaggatgtcc atatctgaag cagttcaggt gccgctatac tgaagatgcg gatgagggaa
    10501 taagggagat ggataagacg gccctggagt tgcttaaaga agaggaaggc gcaaaggtca
    10561 aatggcatga ggtacggaag aggattcctg agctctttcc ttcctggtat aggatatact
    10621 gtactacctg tgggtggaag cgggcatgtg agcgggacgc ttcatatcgg caattgagag
    10681 cttccctttc ttaggattcg atgaaagcaa agaagaatat cattatcgta ggtgacaggg
    10741 ttctcataga acaggacgag aagaccgaca agacgacctc ggggctctac ctccctccca
    10801 ctgtcaagga aaaggagagg gtcttgggcg gctatatcat tagagtcggt cccggctatc
    10861 cggtccatga ccctaacgtg gtgattgaag aaccatggtc ttctcacaaa aggatggatt
    10921 taagatatct tcccctccag gcttctgaag gtgactatgc catcttcctc aaggaggccg
    10981 cagtagaaat cgagtttgaa gggaagaaat atctcatagt tccccattca gcaatcctcg
    11041 ccctggtccg gacagagata accgaagggg aatcttgatg caactctatg aacttcttag
    11101 gaaggagctg ggaggggtaa acaaccgtaa aggagctttc tgattccctg agactggagg
    11161 caagtaccag aggaacaaga ctgctgaacc tctataagaa aagacttgcg agaaaaactg
    11221 atgagaggag aaaaggagga agagtctggg tgtatgagaa agtgtaacat cagagggcag
    11281 acatggaaat aatcgtttat atcttttttg gactgattct cgggggagcc gtggggtggt
    11341 tttttgcg
//
LOCUS       DATKOU010000271        16593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_3454938, whole genome shotgun sequence.
ACCESSION   DATKOU010000271 DATKOU010000000
VERSION     DATKOU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454938"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1940
                     /gene="thrS"
                     /locus_tag="VMU21_12950"
     CDS             168..1940
                     /gene="thrS"
                     /locus_tag="VMU21_12950"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HUO78474.1"
                     /translation="MCIISNCVAGGAEITIKSEEDIETYRHSTSHIMAHAVKDLFPDA
                     KLAIGPAIEDGFYYDFDISRPLTPEDLEKIEGKMAEIIKDNKPFNRKILSRKEAIELF
                     RKQGEEYKVELLNEITDEQVSVYEEGGFVDLCRGPHVSSTGVVRAFKLLSIAGAYWRG
                     NEKNRMLQRIYGTSFGDAKALKEYLVFLEEVKKRDHRRLGKELDLFSISDEIGPGLIL
                     WHPNGAIIRREIEDFWREEHSKAGYQILFTPHIARLNLWQKSGHWDFYRENMYSPMEI
                     EGMEYELKPMNCPFHLYIYKSSLRSYRDLPIRYAELGTVYRYERSGVLHGLLRVRGFT
                     QDDAHIFCREEQIEDEILKVLDFTLFILKTFGFDEYDVYLSTRPEKYVGTLENWERST
                     EALKRALEVKGLSYSIDPGEGVFYGPKIDIKVKDSLGRAWQCSTIQVDFNNPQQFDVS
                     YRGSDGKEHKPIMIHRALMGSLERFFGILIEHYAGAFPVWLAPVQASVLTIAERHGEY
                     ATQAAERLKAEGIRTGVNLDNEKIGHKIRGATIRKVPYLGIIGDKEIAEGTLSVRRRN
                     GEDLGAMTQENFIAMIKEDIRLRR"
     gene            1969..2451
                     /gene="infC"
                     /locus_tag="VMU21_12955"
     CDS             1969..2451
                     /gene="infC"
                     /locus_tag="VMU21_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HUO78475.1"
                     /translation="MRVNERIFAKTVRLIDADGQQAGVVPVKEALRMAEEKELDLVEV
                     APNSVPPVCKIMDFGKYKYQLAKKHSSGKKIEVKEVKLRPQIGEHDLELKARNIRRFL
                     DDGNKAKVTMFFRGRELVRPDLGMKVFERLLHYLDGKFSTEQPPKMEGKNITMVVAPK
                     "
     gene            2464..2661
                     /gene="rpmI"
                     /locus_tag="VMU21_12960"
     CDS             2464..2661
                     /gene="rpmI"
                     /locus_tag="VMU21_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HUO78476.1"
                     /translation="MPKIKTHRGAAKRFKLTGTGKVKRSKANKSHILTGKPAKRMRGL
                     RTATLVAKVDQKNIKTLIPYM"
     gene            2682..3041
                     /gene="rplT"
                     /locus_tag="VMU21_12965"
     CDS             2682..3041
                     /gene="rplT"
                     /locus_tag="VMU21_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HUO78477.1"
                     /translation="MPRAKGGFKTRRRRKKILERAKGYYGAKSRLYRIATEAVDKALQ
                     YAYRDRKNKKREFRSLWIIRINAAARAMGLTYSQFMSGAKKAQIGLDRKTLAHMAYHD
                     PQAFSQITETVKKKLAS"
     gene            3126..4139
                     /gene="pheS"
                     /locus_tag="VMU21_12970"
     CDS             3126..4139
                     /gene="pheS"
                     /locus_tag="VMU21_12970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HUO78478.1"
                     /translation="MADITEIKKAFLDELSAAGTVSDLQLLRVKYFGKKGVLTAQLKL
                     LPTISSEERPAFGKLINEFKTFIEEKTDFKESELKHAELDKKLVSEAIDITLPGNYVP
                     PGSMHPINQVLKEILGIFVGMGFDIKVGPEVELDYYNFEALNMPKDHPARDMQDTFYI
                     TDEVLLRTHTSPVQVRIMEKTKPPLKFVAPGKVYRCDADVSHTPMFHQVEGLMVDSDI
                     SFSDLKGVLELFIRRMFNKDIPVRFRPSFFPFTEPSAEVDIGCILCAGSGCRVCKGSG
                     WLEVLGAGMVNPRVFEMVGYDPDIYTGFAFGMGVERITMLRYSIDDIRMFYENDIRFL
                     RQF"
     gene            4236..6296
                     /gene="pheT"
                     /locus_tag="VMU21_12975"
     CDS             4236..6296
                     /gene="pheT"
                     /locus_tag="VMU21_12975"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HUO78479.1"
                     /translation="MRVPIEWLKEFVEFSLSPEELALKLTMIGLEVESLEKAYDDTVL
                     EVNVTPNRPDCLSILGIAREVSAMINSPLRFPECGIQAESGPSSVAVVIADEDLCLRY
                     AGRDIEDVAIGPSPEWMKRRLEQCGMRPINNIVDITNYVLMEMGHPLHAFDKEKLKGG
                     RIRVARAIPGSRVTTLDGSVRDIPDGSLLIWDAERPVAVAGVMGGAESEVEEHTKQIF
                     LESAYFLPASVRRTSKALGLKTESAYRFERGTDIEFLEKALDRAAFLMATLAGGRVSK
                     KVDVYPKPFEPPKITVRFERVNKILGTSIIPDDMLDIMKRLGIEVLQADTKAFTITPP
                     SYRRDMEREIDVVEEVARFHGYEKIPVTVPKIHTSGGGTDRRHEHTSTVKTALVVEGF
                     TEAVNYSFMNYQALDMLSLGSDDPRRRTLTLRNPLNEDESCLRTTLVPSLVKNLVVNV
                     STGSRDVRLFEISRVYIDQGESLPKEEHHLGVLYFKEKGPSLWKDETPDFFLVKGVVE
                     SLMRGLRIQGYTFEPSTEPFLHPGKSCDIKVSGVRIGFLGLLHPQIVEKLGLKVSRKD
                     IVLLEIDLDLLFNFVPKTATYTPIPKYPGIERDVALIVDESLPAFTIIAEIRAYPSEL
                     MEDVVLFDFYKGKNIPEGKKSLAFTVRYRAKDRTLTDSEIEELHAGLVTYVTTRTGGV
                     VRGA"
     gene            complement(6388..6591)
                     /locus_tag="VMU21_12980"
     CDS             complement(6388..6591)
                     /locus_tag="VMU21_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HUO78480.1"
                     /translation="MADTSIKVEGMSCQHCVMRVTKALQGLSGIQDLDVQIGEVKLRF
                     DEHLLKKEDIVKAIENAGYKVTR"
     gene            complement(6673..7410)
                     /locus_tag="VMU21_12985"
     CDS             complement(6673..7410)
                     /locus_tag="VMU21_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78481.1"
                     /translation="MGNWLDFNAGKTYLKFVPSRARYYIRCPYFLYLSNNKEIYIPYS
                     QIARQKGNFFEKIVLNRISEKTGYEISKAKQVTDLLKRGGLYTLNKQVDTEFYVTENV
                     VFKVGMLKPDLILSEKAKNSIEITIIEIKHSDDLKVYHYLQAYIYKLTLEKLLSDKIK
                     MPIHIRVSMIHREKGCYPQDEYESDFSSFKERLVTMNLESFIVENYTDIESEIVLSQA
                     LQNIASLQANTAECNACPGAGQCEHAN"
     gene            complement(7395..10229)
                     /locus_tag="VMU21_12990"
     CDS             complement(7395..10229)
                     /locus_tag="VMU21_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HUO78482.1"
                     /translation="MTNTLIIGPQENLIERTASLIGPLRKDYSHNLVVFPGRRPAHFL
                     RRELAQRGKGSFIPPLILSMDGFMDYVTEAVHPMRHMETVDAIAILYQIHRSAPEPLG
                     GESFLTLDSFFSLGIKLFADIEELYIEGVPPAMVKEIQPFTDQMIPKHALARLQSLHF
                     FYKTFYEKISQLGMATRSMRYRMAAERIPESGIDRFEKVIFAGFYALTRCEKDLFRKL
                     LPSEKAIFIFQDGPGLRERLSELGIETKDVQPSHPEPEIHFYSSPDTHGQVFALGSIL
                     REKSEKKGLPDEKTTIVLPSSETLFPLLRQGMTCIDKDDFNISLGYPLHRTPVFGFLN
                     NLMELIGSMEDDRFYVPDYMKFILHPYMKNIYCKGNAEITRIMFHTIEEILQKSRTRM
                     FATLSDIENNEKILNSITEKLRQEEEGMTRAEIREHLRMVHLNTIEKFLSFRDIGDFA
                     AKCTELLTFVFSNSTARLHPLFYPFSESFIRALDTVSKSLMKDMSFTDRTSYFTFFRK
                     YIMTGHVPFPGTPLKGVQILGFLETRNIRFDTVFILDANEEVIPDTSKEDTLLPFKAQ
                     EILKLPTYLDRDKLSAYYFYNLVKGAKEVHIFFVENDTKERSRFVESLLWERQKRDRS
                     TRTSDYVQAVQYKVKLGNDTPAGIPKTDRVVSFLKDFTYSATVLDTYLNCPLQFYYSY
                     VLDLGKKEELTGEIERADVGKFVHRVLSSYFSKRLNRVLKEADIDLEEMDNLIDDLFS
                     KEYGSNPTGAVYLLKAQIKEHFREYLSKYRIPLIKKKKVTIIGVEEPIEATLNSYKLK
                     GRLDVVEKRDGNICIVDYKSGSYAKNLKINFNKLDPGSRDSWDEAIGSLQLPFYLILY
                     SERHGIKIEDLRGMFMLLGRSLINREIELPLFDTDDESSTFDGLRTVILGLLEEIVDP
                     DVPFGAASNRKTSCPKCDFQYVCGTQWVTG"
     gene            complement(10226..13309)
                     /locus_tag="VMU21_12995"
     CDS             complement(10226..13309)
                     /locus_tag="VMU21_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUO78483.1"
                     /translation="MEVPIIEKDSELKFPHFTVLKASAGSGKTHMLTKRFVQFLLSER
                     IPRNSLRNILAITFSNNAAKEMREKIIDWLKLLHFGDPELTEQMLESMSMDREKLAER
                     AGQMMEGILWDYADFEVRTIDSFMTSIFKTSAIDFGFNTEFDILMNNDALMEYSFDLF
                     LRGVREGTREAELLLSIISAIQEGKQGQEAYMWDPSSVLLEEVKSLYGKLSATGKNPM
                     IEDYSGAFDDIKDEISTATEGLEKVILKSGLVKRANSSFDTVLDLVKEGRFADLIGKG
                     LKNPPVNKPGKGQSPLQTSFDQIVGQWENAASLINKYIALLVRSRSMPYLKVYESFRE
                     IVESVKRRQGKIFIEDISRNLAEYLNEEIVPDIYFRIGETIYHFLIDEFQDTSLIQWH
                     NLFPLFENALSQGGSLFVVGDTKQAIYGFRNADYTIMREAERTNPFPSARHYVKELTT
                     SRRSLKKILDFNEKVFKDIVAGNDKYKEAGTRSGLTDYAQCARDGSSQGYCEVVLQER
                     DDETPPERRVLQDLVAELRSRGFSYGDIAILTRRNEDVVKTTLWLNEKNIDFISYSNL
                     DIRRRKITGEIVALLNFLDSPMDDLSFSTFINGDIFGALLRADHPEITPERLGEFLFG
                     YRESPPLYKSFQKEFSILWEKCFAGLFKASGYFPIYDLVTEAYAVFRVFETLGDEEAA
                     LVKILEAVKEFEGAGYNNIRDFLDFAGDSDSGESEWNMDVPKTLDAVKVMTIHKAKGL
                     GFPAVIVLLYEGRNRGFDYIIEEEENGVRLLKINKKTMVSDPTFERLYEEEATKETVN
                     RLNSLYVGFTRAKEELYVIGVKGRSETFPFDLLPASDFAPTAKPDRCDIPTAKSSGVF
                     SVRHHPKQIEAEVRTDEMISVEERARGEFIHRVLYHVEYLHDDMESVLEDIIRKVNVE
                     TASKSAVEGVKELLLRLFGRKEIATYFDPHPGREVKREQEFSDEKGHLFRMDRVVIDP
                     DRITILDFKTGGEGWADEKYKAQMRKYMKIAKDIYPGRRLEGKIVYIDLGEIRTL"
     gene            13477..13752
                     /locus_tag="VMU21_13000"
     CDS             13477..13752
                     /locus_tag="VMU21_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HUO78484.1"
                     /translation="MKTLTALDLRKKLGSVLDDVSMKGEEVIISRGNKPLAVIISVDE
                     YEEKIQKKHREKRLVDVAASMDRWKKKHLKETKIDTVKGVREIREGR"
     gene            13749..14153
                     /locus_tag="VMU21_13005"
     CDS             13749..14153
                     /locus_tag="VMU21_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUO78485.1"
                     /translation="MIVLDASVVLKWIFGDEDRGEKAKLFRENHASGKDAIAVHNLFF
                     YEIANVLSTKTTLSYKDASDAFSLIWAFDLEVFDFGRNEYLSSIRLSKLYGITLYDAS
                     YIELAHRLKCRFITADRKLHQKVKGAGGIELL"
     gene            14536..15269
                     /locus_tag="VMU21_13010"
                     /pseudo
     CDS             14536..15269
                     /locus_tag="VMU21_13010"
                     /inference="COORDINATES: protein motif:HMM:NF016442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
     gene            15269..16039
                     /locus_tag="VMU21_13015"
     CDS             15269..16039
                     /locus_tag="VMU21_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HUO78486.1"
                     /translation="MLTELKKESQTTLHRLIHGDNRSLSFIADESVHLVVTSPPYWSL
                     KRYNEQRGQLGHIADYERFISELSKVWKEVYRVLVPGGRLVCVVGDVCLSRKEHGRHL
                     VMPLHADITVICRKLGFDNLNPIIWHKISNANFEANDGTKFLGKPYEPNAIVKNDIEF
                     ILMQRKPGGYRKPTQEQRRLSMIDKDDFAEWFRQFWNITGASTRNHPAPFPFELAYRL
                     VRMFSFWGDTVLDPFLRNRHNHACRHQDREKQHRRGDR"
     gene            16026..16220
                     /locus_tag="VMU21_13020"
     CDS             16026..16220
                     /locus_tag="VMU21_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78487.1"
                     /translation="MEIDEEYCRMALHRLRTEGRDLFREAEIEFLRMDDNGVAIVVQE
                     EPAVYGGKEKKKAAMRKRVK"
     gene            complement(16396..>16593)
                     /locus_tag="VMU21_13025"
     CDS             complement(16396..>16593)
                     /locus_tag="VMU21_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUO78488.1"
                     /translation="FGGPLEATVLVRLATAAGILVGIVCVASVTIVVGAIFGTVVGYL
                     LEAVSAPAEVKTSEAAAVKAK"
BASE COUNT         4342 a         3771 c         4170 g         4310 t
ORIGIN      
        1 gcattaaata agcttgcggt cagaagaagg caggagtctt cataaaggga agccattgtg
       61 gaagggtatc ctttcttcat tcttcataaa caatagccat gtagtgcgca tgtcataatc
      121 caacctggat ctgttaaaga ataaggtgtt gaaaatcaag aagaaagttg tgtatcatat
      181 cgaattgcgt agccggaggt gctgaaatta ccataaaatc tgaagaggac atcgagactt
      241 accgccatag cacgtctcat atcatggccc acgcggtgaa ggatctcttt ccggatgcca
      301 aattggctat cggtcctgca atagaagacg ggttttacta tgactttgat atcagcagac
      361 cgttaactcc cgaagacctg gagaaaattg aagggaagat ggccgaaatc atcaaagata
      421 ataagccctt caaccggaag attctttcgc ggaaagaagc cattgagctt tttagaaagc
      481 agggcgaaga gtataaggtt gagctcctca atgagattac cgacgagcag gtctcggtct
      541 atgaagaggg tggatttgta gacctctgcc gcggacccca tgtttcctca acaggggtgg
      601 tcagggcatt caaactcctg agcattgcag gggcatactg gagagggaac gaaaaaaaca
      661 ggatgttgca gcgtatatac ggaacctcct ttggggacgc gaaagcgctc aaggaatatc
      721 ttgtgtttct tgaggaggtc aagaagagag accatcgaag gcttggcaaa gagcttgatt
      781 tgttcagcat aagtgatgaa atcggtccgg gtttgattct gtggcatccc aatggcgcca
      841 ttatcagacg ggaaatagag gatttttggc gtgaagagca ctccaaggcc gggtatcaga
      901 ttctctttac tccccatatc gcaagactga atctctggca gaagagcgga cactgggact
      961 tttaccgcga aaatatgtat tcccctatgg agattgaagg catggagtat gaattgaagc
     1021 ccatgaactg cccctttcat ctctacattt ataagagttc tctgaggagc tatcgagatc
     1081 tccctatcag gtatgctgaa ctgggcactg tgtaccgtta cgaacgttcg ggtgttttgc
     1141 acggattgtt gagggtgagg ggctttaccc aggacgatgc ccatatattc tgccgggaag
     1201 agcagatcga ggatgagatc ctaaaggtac ttgattttac actctttata ttgaagacgt
     1261 ttggatttga tgagtacgac gtctaccttt ccacaaggcc ggagaaatat gtgggcacgc
     1321 ttgagaattg ggagcgttcc acagaggccc tcaaacgtgc gcttgaggtg aaagggcttt
     1381 cctattccat agatcctggt gaaggggttt tctacggacc gaagatcgat atcaaggtga
     1441 aggattctct cgggagggca tggcagtgca gtactattca ggtggatttc aacaacccgc
     1501 aacagtttga tgtgagctat agaggcagtg acgggaaaga gcacaaacct atcatgatcc
     1561 acagggctct catggggtcg ctggagaggt tctttggcat cctcattgaa cactacgcag
     1621 gtgcctttcc tgtctggctt gcaccggtcc aggccagcgt gctcaccatt gcggagagac
     1681 acggcgagta tgcaacccag gctgcagaga ggttgaaggc cgaagggata aggaccgggg
     1741 taaaccttga caatgaaaag atcggtcata agataagggg agccactatc agaaaggtcc
     1801 cgtatttggg tataataggc gataaggaaa tcgcggaagg aaccctttct gtgagaagac
     1861 ggaacgggga agatcttgga gcgatgacac aggaaaattt tatcgcgatg atcaaagaag
     1921 atatcagact caggaggtga tagtataagt aaggacatcg gaaaagagat aagggtaaac
     1981 gagaggatct ttgccaagac ggtcaggctg attgacgcag atgggcagca ggcaggggtg
     2041 gtgccggtaa aagaagcgtt acgcatggca gaagagaaag agctcgatct cgtcgaggtc
     2101 gcaccgaatt cggtcccgcc ggtatgcaag ataatggatt tcggcaaata caaatatcag
     2161 ttggccaaaa agcatagctc agggaaaaag atagaggtga aagaggtgaa actgagaccc
     2221 cagataggcg aacatgatct tgaattgaag gcaaggaaca taaggcgttt cctggatgac
     2281 ggaaataagg ccaaggttac catgttcttc aggggaaggg aattggtgag gcctgatctc
     2341 ggcatgaagg tctttgagag attgctgcac tatcttgatg gaaaattcag tactgaacag
     2401 ccgccaaaaa tggagggcaa aaatataacc atggtggttg caccaaagtg acaggaggtt
     2461 tcgatgccca aaatcaagac gcatagagga gctgcaaaga gatttaagct tacagggacg
     2521 gggaaggtca agcgtagcaa ggccaacaaa agtcatatcc tcaccggaaa gccggcgaag
     2581 aggatgcgag gtctgaggac tgcgacgctg gttgcaaagg tcgatcagaa aaatatcaag
     2641 acactcatcc cttatatgtg agagtcgaga aggaggaata gatgccaagg gcaaagggtg
     2701 gttttaagac aagaagaaga aggaaaaaga tactggaaag ggccaagggc tactacggag
     2761 cgaaaagtcg tctctacagg attgccacag aggccgttga caaggccctg cagtatgcgt
     2821 acagggaccg aaagaacaag aaacgggaat tcagatccct ctggatcatc aggattaacg
     2881 ccgcggcaag ggccatggga ctcacctaca gtcagtttat gtcaggagcg aagaaggcac
     2941 agatcgggct tgacagaaaa acgctggccc atatggccta tcatgatccc caggcattca
     3001 gccagattac agagaccgta aagaagaagc tggcgtcata aatcagcgcg ggcgataacg
     3061 atcatttcaa ggccctcctc aatctcccta tgcgagagaa tgaaggaggg tagctttttt
     3121 tcttcatggc agatataacc gaaatcaaaa aggcatttct tgatgaactc agtgctgcag
     3181 ggacagtgag tgacctccag cttctgaggg tgaagtactt tggtaagaaa ggggtactca
     3241 ctgcgcagct taaacttctc cccaccatat cctcggagga aagacccgcg tttggaaagc
     3301 ttattaacga attcaagaca ttcatagagg aaaagacgga tttcaaggaa tcggaactaa
     3361 agcacgcaga gctcgacaaa aagcttgttt ccgaagccat tgatatcaca ctgccaggga
     3421 actatgtccc tcccggaagc atgcatccca tcaaccaggt tcttaaagag atcctgggta
     3481 tttttgtggg tatggggttt gatatcaaag tgggccctga ggttgaactg gactattata
     3541 attttgaagc cctcaacatg ccgaaagacc atcctgccag ggacatgcag gacaccttct
     3601 atatcaccga cgaggtgctt ctgagaaccc acacctcgcc tgtacaggtc aggatcatgg
     3661 agaaaacaaa gcctcccctg aaatttgtcg caccggggaa ggtctatcgg tgcgatgcgg
     3721 atgtgtcaca caccccgatg tttcatcagg tggaagggct gatggtggat agcgatattt
     3781 cgttcagcga tcttaagggc gtgctcgaac tctttattcg ccggatgttc aacaaggaca
     3841 tccccgtgag gttcaggccg agcttcttcc cttttacaga gccgagtgct gaggtggata
     3901 tcggatgcat cctctgcgca ggaagcggat gcagggtgtg caaagggtcg ggatggctcg
     3961 aggtgctggg cgcagggatg gtgaatccca gggtatttga aatggtggga tatgatcccg
     4021 acatatacac cggctttgcc tttggtatgg gcgtggaaag aatcaccatg ctcaggtatt
     4081 ccatagacga tataagaatg ttctacgaaa acgatatacg ctttctgagg cagttttgaa
     4141 tgctacagag atcaagctca gccccaaggg tacgactgag agggattgtg tataccttat
     4201 tccatcctgt cattattttt ttataaaggt gtgaaatgcg tgttcccata gaatggttaa
     4261 aggagtttgt ggagttttcc ttaagccctg aagaactggc tcttaaattg accatgatag
     4321 gacttgaggt cgaatccttg gaaaaggcgt acgatgacac ggtccttgag gtaaatgtga
     4381 cgccgaacag gcctgattgc ctcagcatcc tgggtatcgc acgggaagtc tcggccatga
     4441 tcaattcgcc cttgcgattt cccgagtgtg gaatccaagc agagagcgga ccatcctccg
     4501 tggccgtagt aatcgctgat gaagaccttt gccttcggta tgcagggagg gatatcgagg
     4561 acgtagcaat agggccctct cctgaatgga tgaagaggag gctcgaacaa tgcggcatga
     4621 ggcctatcaa taacattgtt gacatcacaa actatgtgct gatggagatg ggacatcctc
     4681 tgcatgcctt tgacaaggag aaattgaagg gcggcaggat cagggttgcg cgggccattc
     4741 ccgggagcag ggtaaccact cttgatggat cggtgcgcga tatccctgat ggcagcctcc
     4801 tcatatggga cgcagaacga ccggtagcag tggcgggagt aatgggcggt gcggagtcag
     4861 aagttgagga acacacaaaa cagattttcc tcgaaagtgc ttattttctc cctgcatctg
     4921 tgcggaggac ctcaaaggcc cttggactga agaccgagtc tgcgtaccgg tttgaaaggg
     4981 gcactgatat tgagttcctt gagaaggcac tcgacagggc tgcattcctg atggcgacgc
     5041 ttgcaggggg aagggtctcg aagaaagtcg atgtgtaccc aaagcctttt gagccaccga
     5101 agataacggt cagatttgag agggtgaata agattctcgg tacctccatt atcccagatg
     5161 atatgctgga catcatgaag cggcttggga ttgaggttct tcaagcggat accaaggcct
     5221 ttacgattac ccctccgtca tatcggagag atatggaacg ggagattgat gtggtggaag
     5281 aggtggcgcg tttccatggc tatgaaaaga ttccggtgac ggtcccaaag attcatacat
     5341 cagggggagg cacggataga cgccatgagc atacgtctac ggtaaaaact gcactggtag
     5401 tcgaagggtt tacggaggcg gttaattaca gcttcatgaa ttatcaggcc ctcgatatgc
     5461 tttctcttgg cagtgacgac ccgcggcgca ggactcttac gttacgcaat cccctcaatg
     5521 aggatgagtc ctgcttaagg actaccctgg tgccgtccct tgtcaagaat cttgtggtca
     5581 atgtctccac aggaagcagg gatgtgagac tttttgaaat ctcccgggta tatatagatc
     5641 agggggaaag cctcccgaag gaagagcatc atctgggagt cctctatttc aaggaaaagg
     5701 ggccatctct gtggaaggac gaaacccctg attttttcct ggtaaaaggg gtggtggagt
     5761 ccctcatgcg cgggctaagg attcagggct acacgttcga accttccact gagccctttc
     5821 tgcatcctgg aaagtcctgt gatataaagg tgtcgggcgt gcggatcgga tttcttggat
     5881 tgcttcatcc ccagattgtg gaaaagctcg gtctcaaggt ctcacgaaaa gacatcgttc
     5941 ttcttgagat tgaccttgac ctccttttca actttgtgcc gaaaaccgcc acgtataccc
     6001 ctatccctaa atatccagga atcgaacggg atgtcgccct tattgttgac gaatctcttc
     6061 ccgcatttac aataattgcg gagataaggg cctatccctc cgaactcatg gaagatgtag
     6121 tgcttttcga tttctataag gggaagaaca ttcctgaagg gaagaagagc ctcgcattta
     6181 ctgtccgcta cagggcaaag gataggacac tcactgattc agagatagaa gaacttcatg
     6241 cggggctggt cacctatgtg accaccagaa ccgggggcgt tgtccggggg gcataacgcc
     6301 cgagaaactg cggggcctgc agggaaacag attcaggctg aggctgagga aatcatagga
     6361 cgttgccatg agcctgccct taaggtctca gcgcgtgact ttatacccgg cattctcaat
     6421 tgcctttacg atgtcctctt tttttagtaa gtgttcatcg aacctgagct tgacctctcc
     6481 gatctgcaca tccagatcct gaatgccact gaggccctgt aatgcctttg tgaccctcat
     6541 cacgcaatgc tgacagctca ttccttcgac cttaatactg gtatcggcca tatatcctcc
     6601 tctggcagcc ttgtctcctt gatatctagc gactattcta taagaagaca gttgagaaag
     6661 acaacgtgcc cttcaattag catgttcaca ttgacctgcc cctgggcatg cgttacactc
     6721 tgcggtgttt gcctgtaatg aagctatatt ttggagagct tggctcaata caatttcgga
     6781 ttctatatct gtataattct caacgataaa agattcaagg ttcatcgtta caagccgttc
     6841 tttaaaactc gaaaaatctg attcatattc gtcttgagga tagcatccct tttcccggtg
     6901 tatcatgctc actctgatat gaatgggcat ttttatttta tctgacaata atttctccaa
     6961 cgtcagcttg taaatgtaag cctgcaaata gtgataaacc tttaagtcat cagaatgctt
     7021 tatctctatt atggttattt caatactgtt ttttgccttt tctgacagaa tgaggtccgg
     7081 cttaagcatc cccaccttga aaacaacatt ttcagtaaca taaaattccg tatccacttg
     7141 tttgtttaga gtatagagac cacccctttt gagaagatca gttacttgct tagccttgct
     7201 tatctcatat cccgttttct cagagattct gttgagaaca attttttcaa aaaagtttcc
     7261 cttctgtctc gcaatttgag aataaggtat atagatttct ttattattgg aaagataaag
     7321 aaagtatgga catctaatat aatatctcgc ccgtgagggc acaaacttaa gatatgtctt
     7381 gcctgcgttg aaatctaacc agttacccat tgtgttccac aaacgtactg gaagtcgcac
     7441 ttcggacaac tggtctttct gttcgatgcc gctccaaatg gaacatctgg atcaactatc
     7501 tcttccagaa gacccaaaat cactgttctt aatccgtcga atgtcgaact ctcgtcgtct
     7561 gtatcgaaca agggaagttc aatctctctg tttatcaagg accggccaag gagcataaac
     7621 atccctctca ggtcctctat ttttatgccg tggcgctcag aatataaaat aagataaaaa
     7681 ggaagctgga ggcttccgat cgcttcatcc caggaatccc tgctgccagg atcaagcttg
     7741 ttgaagttga tctttaggtt ctttgcatag gaaccgcttt tataatcaac tatgcagata
     7801 ttaccatctc ttttttcgac tacgtccagc cgacctttga gcttgtagga atttaaagtc
     7861 gcttcgatag gctcttccac tccgataatc gtcactttct tctttttgat aagaggtatg
     7921 cggtacttgc tgagatactc tctaaaatgt tctttgatct gtgctttcag aaggtaaacg
     7981 gccccagtag ggttagagcc gtattcctta ctgaaaagat cgtcgatcaa attatccatc
     8041 tcctccaaat cgatatccgc ttcttttaga actctgttca gcctttttga gaaatatgat
     8101 gaaaggacgc ggtggacgaa cttgccgaca tcggctcttt cgatttctcc tgtgagttcc
     8161 tccttcttcc cgagatcaag tacgtagctg tagtagaact gaagggggca attcagatat
     8221 gtgtcgagga ccgtcgcact atatgtgaaa tcttttaaga agctgactac cctgtcggtt
     8281 ttaggaatgc cggcaggtgt atcgttgccg agctttactt tgtattggac agcctgcaca
     8341 tagtcacttg tcctggtaga tctgtccctt ttctgtcttt cccataggag actctctaca
     8401 aagcgtgatc tctccttggt atcattttcc acaaagaaaa tgtggacttc cttggcgcct
     8461 tttacgagat tgtagaaata gtaggccgac agcttatccc tgtccaaata ggtcggcagt
     8521 ttcaggattt cttgcgcctt gaaaggcaga agcgtatctt ctttggaggt gtcagggatc
     8581 acctcttcat tcgcatcgag aataaaaaca gtgtcaaacc tgatgtttct cgtttcaagg
     8641 aaaccgagga tctggacacc cttaagcgga gtgccgggaa agggcacgtg accagtcata
     8701 atgtattttc taaaaaatgt gaaatagctt gtcctatccg tgaacgacat gtccttcatc
     8761 aaggatttgg agactgtgtc cagggctctt atgaaggact ccgaaaaagg gtagaagagg
     8821 ggatgaaggc gggccgtact gttgctgaag acaaatgtga gaagttcggt gcattttgcg
     8881 gcaaaatccc cgatatccct gaaggagaga aatttctcaa ttgtgttgag atggaccatc
     8941 cttaagtgtt ccctgatctc cgccctggtc atgccttctt cttcctgacg aagcttttca
     9001 gtgatgctgt tgagtatctt ttcgttgttt tctatgtcag acagtgttgc gaacatcctg
     9061 gtcctggatt tttggagtat ctcctcaatg gtgtgaaaca taatcctcgt gatctcggcg
     9121 tttcccttgc agtaaatatt cttcatgtaa gggtgtagta tgaacttcat atagtcaggg
     9181 acgtaaaacc ggtcatcttc catggaacca atcaattcca tgagattatt aagaaagccg
     9241 aataccggcg tcctgtgcaa tggatatccg agagagatgt tgaagtcgtc cttgtctata
     9301 catgtcatcc cctgacggag aaggggaaag agggtctccg aagacggcag tactattgta
     9361 gttttttcgt cgggcaatcc tttcttctca gatttttccc tgaggatgct tccaagggcg
     9421 aagacctggc catgggtatc ggggctgctg tagaaatgga tttccggttc aggatgagag
     9481 ggttggacat ccttggtctc tattcccagt tccgacagtc gctctctcag accaggacca
     9541 tcctggaata tgaagattgc tttttcgctc ggtaacagct tcctgaagag atccttctcg
     9601 cacctggtca acgcatagaa tcccgcgaat atcacctttt caaacctgtc aatgccgctt
     9661 tcggggatcc tctccgctgc catgcggtat ctcatggatc ttgtcgccat acccaattgg
     9721 gaaatcttct cgtaaaaagt cttgtagaaa aaatgcagtg attgaagcct cgccagtgca
     9781 tgcttgggta tcatttgatc agtgaagggc tggatttcct ttaccatggc aggaggcacc
     9841 ccctcaatat agagttcctc aatgtccgcg aaaagcttta ttccaagaga aaagaagcta
     9901 tcgagagtga ggaagctttc tccaccgagg ggttccgggg cgctcctgtg aatctggtag
     9961 aggatggcaa tggcatcgac tgtctccata tgcctcatgg ggtgcacggc ttccgtaacg
    10021 taatccatga atccgtccat ggacagaatt aagggaggta tgaagctacc ctttcccctt
    10081 tgtgctaact cccttcgaag gaaatgcgcc ggtcttctgc ccggaaacac gacaaggttg
    10141 tgagagtagt cttttctgag agggcctata aggcttgctg tcctctcgat aaggttttcc
    10201 tgtgggccta tgatcagcgt attggtcata atgtcctgat ctctccgagg tctatataaa
    10261 cgattttgcc ttccaggcgt ctaccaggat aaatgtcctt cgcgatcttc atatattttc
    10321 tcatttgtgc cttgtatttc tcatcagccc agccttctcc cccggtcttg aaatccagga
    10381 tcgtaatcct gtccgggtca atcacgactc tatccatcct gaagagatgt cctttctcat
    10441 ccgagaattc ctgctccctt ttcacctctc tcccgggatg gggatcgaag taggtcgcta
    10501 tctccttgcg tccgaagagg cgaagaagaa gctctttcac tccctccacg gctgactttg
    10561 atgctgtttc gacgttgact tttctgatga tgtcctcaag cacgctttcc atgtcatcat
    10621 gcagatattc gacgtggtaa agaaccctgt gaatgaattc tcccctcgcc ctctcttcga
    10681 cgctgatcat ctcatccgtt ctgacctctg cttctatctg tttggggtga tgtctcacac
    10741 tgaagacccc ggaagatttt gcagttggaa tgtcacatct gtcaggtttt gccgtgggtg
    10801 caaagtcgct tgcgggaagg agatcgaatg gaaaagtctc gcttctgccc ttcacaccga
    10861 tgacgtagag ctcttctttt gcccgcgtga atccgacata aaggctgtta agcctgttca
    10921 cagtttcctt tgtcgcttcc tcttcatata gcctctcgaa agtgggatcg ctgaccatgg
    10981 tcttcttatt gatcttgagc agccgcaccc cattttcttc ctcctctatg atgtagtcaa
    11041 agcccctgtt gcggccttca tagagaagga caatgactgc ggggaatccg aggccctttg
    11101 ccttgtgaat cgtcatcact ttgacagcgt ccagggtctt ggggacgtcc atattccact
    11161 cggactcgcc gctgtcgctg tcgcctgcaa aatctaggaa gtccctgatg ttgttatagc
    11221 cggccccctc gaactccttg accgcttcga gtatcttcac aagtgccgcc tcctcgtccc
    11281 ccagtgtctc aaagaccctg aagacagcgt aagcctctgt cacaaggtcg tagatcggaa
    11341 aataccctga ggccttgaaa aggccggcga agcatttctc ccataaaata gagaactctt
    11401 tctggaatga cttgtagaga ggagggctct ctctatatcc aaaaagaaac tccccgagtc
    11461 tctccggagt aatctcagga tggtccgcac gtagcaaggc gccgaagata tctccgttta
    11521 tgaaggtaga aaaagagagg tcatccatgg gcgaatcaag gaagttcagc agcgccacta
    11581 tctcgccggt tatcttcctt ctccgtatgt cgaggttgct gtaagaaatg aagtcgatgt
    11641 tcttctcgtt cagccagagg gtggtcttta cgacgtcttc atttctccgg gttagtatgg
    11701 ctatatcacc atagctgaac ccccgcgatc taagttctgc gacaaggtcc tgtaagacgc
    11761 gccgttctgg aggggtttcg tcatcccttt cctgcagcac cacttcgcag tatccctgcg
    11821 agctgccgtc tctggcgcat tgggcgtagt cggttagccc gcttctggtc ccagcctcct
    11881 tgtatttgtc atttcccgcc acgatatcct tgaatacctt ctcattgaag tcaaggatct
    11941 tcttgaggct tctgcgactt gtcgtaagtt cttttacgta atgtctggcg gaagggaagg
    12001 ggttggtgcg ctcggcctct ctcatgatcg tgtagtcggc gtttcggaag ccgtagatcg
    12061 cctgtttggt gtcgcccacg acgaagaggc ttccaccctg agacagggcg ttctcgaaaa
    12121 gggggaaaag gttgtgccat tgaatgaggg aagtgtcctg gaattcgtca atcaggaagt
    12181 ggtagattgt ctcgccgatc ctgaaataga tgtcgggaac gatttcctcg ttaaggtatt
    12241 cggcaagatt tctgcttatg tcttcgatga atatcttccc ttgacgtctc ttgacggatt
    12301 cgacgatttc tctgaatgat tcatatacct tgagatacgg catggaacgg gagcggacga
    12361 ggagggcaat gtatttgttg ataagactgg cggcgttctc ccactgtccg acaatctgat
    12421 cgaatgaggt ctgaagaggg gattggcctt ttcccggctt attgaccggt gggttcttaa
    12481 gcccctttcc gatgagatcc gcgaaccttc cctccttcac gagatcgagg accgtatcga
    12541 aagaagagtt tgcccgtttt acaagccctg acttgaggat cactttttca aggccctcag
    12601 tggcggtact tatttcatct tttatatcat caaaggcccc tgagtagtcc tctatcatgg
    12661 ggttctttcc ggtcgctgag agctttccgt agagactttt cacctcttcg aggaggactg
    12721 atgaagggtc ccacatatag gcttcctgtc cctgtttccc ttcctggatc gctgagatta
    12781 tactgagaag gagttctgcc tctcttgtgc cctctcggac gcccctcaag aacaaatcaa
    12841 aagagtattc catgagcgca tcattgttca taaggatatc aaactcagtg ttgaatccga
    12901 agtcgatggc ggaggtcttg aagatcgatg tcatgaagct gtctatggtc ctcacctcga
    12961 aatcggcgta atcccatagt attccctcca tcatctgccc ggctcgctcg gccagtttct
    13021 cccggtccat cgacatggat tcaagcatct gttcggtcaa ttccgggtcg ccgaaatgaa
    13081 gcaattttag ccagtcgatg atcttctccc tcatctcctt ggcggcattg ttcgagaagg
    13141 ttatggcaag aatatttcgc aggctgttcc ttggtattct ttcggagagt aagaactgaa
    13201 cgaacctctt ggtgagcatg tgggtcttac ctgagcctgc ggaggccttc agcacagtaa
    13261 aatgagggaa tttcagctca gaatcttttt ctataatagg gacttccact gtatattaat
    13321 agtacgctca agagatttca ttttgcaacc cgggactgaa caggagttcc cccaaaagtt
    13381 gttgactttc agcaaaaact gtcaattctt tcgagtgtca attttctatt gacatagcaa
    13441 ctatcatagt ctattttata gtaaaaggag ggcggcatga aaactttaac ggcgctcgat
    13501 ttaagaaaaa agctcggttc tgttcttgac gatgtatcga tgaaaggcga ggaggtaatc
    13561 atatcaaggg gaaataagcc ccttgccgta ataatctccg ttgatgaata cgaagaaaaa
    13621 atccagaaga aacacagaga gaagagactc gtggatgtag cggcaagcat ggacagatgg
    13681 aagaagaaac atcttaaaga aacaaagata gatactgtaa aaggcgtcag ggaaatcaga
    13741 gagggcagat gatagtcctc gatgcatccg ttgtccttaa atggatattc ggggatgagg
    13801 acagggggga aaaggcaaaa cttttcaggg aaaaccacgc ttcaggcaaa gatgcgatag
    13861 ctgttcacaa cctttttttt tatgagatag caaatgtcct ttcaaccaaa acaactctga
    13921 gttataagga tgcttccgat gccttctccc tcatttgggc attcgatttg gaggtctttg
    13981 atttcggacg aaatgaatac ctcagttcaa tacgcctttc aaagctctat ggcataaccc
    14041 tctacgatgc atcatatatc gaacttgcac atagattgaa atgcaggttc attactgccg
    14101 atcggaaact ccatcaaaag gttaagggag caggcggaat tgaacttctg taattcttgt
    14161 caagagcttt ccacacccgc tggaaaagag tccttgtctt taacatggca taacgtttga
    14221 ccccaaaggg tacaagaaat tacttcaaat tgccaaaact atcgataatt taatacaata
    14281 tggccagcag atgttcttct tttacaagtt atgagctatg agcttttttg ggagggtaac
    14341 ggcaacttga ataatgagtt aacattcctc agtggcagat cagacaccgt cattcttgaa
    14401 ctattcagaa gaaacgcgga tgatgatgga tcaaatatac tcgcaccctt tatagctacc
    14461 ttctggcgaa agcttttctg gtattgttag cggtgcatcc ttgcggcata accgcgaaga
    14521 agcatagaat agccgatgga gataattata aagaatctta acgaaagagt ggctaaggcg
    14581 gtctctcatt actggttgac ccgtgaaaat caggccagaa aacaggaagg atcgggacgt
    14641 gcggaccaag ggttgagaag cgccgtaaca ggcggcgcac agatggatga ctttatcggc
    14701 ttgttgactg aaataatagt tgaggccgga atcgatgagc agcatgtttt tcataagaag
    14761 aatcttgaac tacccggttt tttcagaccg acaaaagaat gggacctcct ggttgttaag
    14821 gatggtcagt tgatcgttgc gcttgaagca aaatcgcaag ttggcccttc ttttggcaac
    14881 aatttcaata accgcactga ggaagctatg ggcagtgccc ttgacctttg gacagcatac
    14941 agagagggtg cttttaacaa aaaaatacgg ccatggctcg gtatatcttt ctgcttgaag
    15001 actgcaaaga atctcaaagc gcggtaagag tgaaagaacc acattttagt gtctttgagg
    15061 aattcagggg cgcgtcatat gcaagaagat atgagctttt ctgcaggaag cttgtaaggg
    15121 agcggcacta caatgcttcg gcctttcttc tctccgacag gaaatccggt aaaaaaggca
    15181 acttcagaga acctgctgaa gacctcacat ccgaacttta catacggtcg ctcgtggcgc
    15241 acgttgctgc acacagtaga ggacgttgat gctcaccgaa ctaaaaaaag aaagtcaaac
    15301 taccttgcat cgtcttatcc acggcgacaa ccgctcacta tcctttattg ctgatgaatc
    15361 cgtacacctt gtcgttactt cgccgcctta ctggtcactg aagagataca atgaacaacg
    15421 gggccagctc ggacacatcg ccgattatga gcgttttatc tcggaattgt cgaaagtctg
    15481 gaaagaggtc tatcgggttc ttgtccccgg cggtaggctt gtctgtgttg tgggagatgt
    15541 ctgtttgtcc cgaaaggagc acggaaggca cctcgtaatg ccgctccacg cggatataac
    15601 cgtcatatgc cgcaaactag gttttgacaa tcttaatccg attatctggc acaagatttc
    15661 gaacgcgaat ttcgaggcaa acgacggcac aaagtttctg gggaagccct atgaacccaa
    15721 cgccatcgtg aagaatgaca tcgagtttat cctgatgcag cgtaagcccg ggggatacag
    15781 gaagccgacg caggagcaac gcagactaag tatgatcgat aaggatgatt ttgccgaatg
    15841 gttccggcag ttttggaaca ttaccggagc gtccacaaga aatcatccgg ccccctttcc
    15901 ttttgagctt gcttaccgac tcgtgaggat gttttccttc tggggcgata ccgtgcttga
    15961 tcctttttta cggaacaggc acaaccatgc ttgccgccat caagacaggg agaaacagca
    16021 tcggcgtgga gatcgatgaa gaatattgcc gcatggcgct ccatcgcctc cgcaccgaag
    16081 gccgcgattt gtttcgtgaa gccgaaattg aatttttgag gatggacgac aacggagtcg
    16141 ccattgttgt tcaggaggag cctgcggttt atgggggaaa agagaagaag aaggcagcaa
    16201 tgaggaagcg ggtaaaatga gaaaaagaaa agaggaagat tgcggcgtct tgaaacataa
    16261 gaaaagtacg ccggataaaa actgatcttt caagccctct gtttcgcttt accagtgagc
    16321 ggccataaaa ataaacgtcc ggaagggaag tactgtcacc ctccgaacgt cttgctttct
    16381 ctcgtaatgg tatttctatt ttgccttaac tgcagccgct tctgaggtct tgacctctgc
    16441 cggagcactc acagcctcca ggaggtatcc cacgactgta ccgaatatgg cacccaccac
    16501 gatggtcact gatgccacac agacgatacc cacaagaatg cctgctgcag tggccagcct
    16561 caccagcacc gttgcctcca agggccctcc gaa
//
LOCUS       DATKOU010000272         9397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4614363, whole genome shotgun sequence.
ACCESSION   DATKOU010000272 DATKOU010000000
VERSION     DATKOU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4614363"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VMU21_13030"
     CDS             <1..1194
                     /locus_tag="VMU21_13030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HUO78489.1"
                     /translation="STYVRVDIEKLDRILNTISELNLARSATNRIAAEMMDTFGHSAL
                     VSDIFKISQTFERRIADITEQVLEIRMIPIGQIFSRLGQVIRRYSRDSGKQIELALYG
                     EDTEIDKYLAEEIIDPLMHIVRNAIDHGIETAEERKRAGKAEAGSIVLKAFQRGNHVV
                     IEVGDDGSGIDLEQVRNTALGKGLITPGAELNDREILDFIFMPGFSTKSTVSEISGRG
                     VGMDVVKEKVSSLGGFVDVETVKGKATTFVLTVPITLAILKALIVRIGPERFAIPLTS
                     LSESLLIEHRSIQTIEGKEVYNLRGEMLPIVGIAKMFGLEADVAEKSYAVVVGYGERR
                     IGLSVDELIGQHEIVIKSLGDYFRGMRGFAGAAEIGKHEVILVIDVESIIEEALSKQK
                     VIHHV"
     gene            1187..2017
                     /locus_tag="VMU21_13035"
     CDS             1187..2017
                     /locus_tag="VMU21_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUO78490.1"
                     /translation="MYEVFYGFRTRPFSKTPDPRFLFMSRTHEEALARLQYAVEEREI
                     AVLTGEIGCGKTTLTRALMDSLDDKYRVMFILNPRLTSFDFVKTISERLQCSISSPHK
                     SDLLEAIYAKLYEEYRSGITPVMIIDEAHHIPYRETFEEIRLLTNFQMDDTNLLGLVL
                     VGQPDLRRKLELPSLRALKQRIGLFCHLDTIGEDEIRSYIDHRTAVGGRNTPLFTEGA
                     LRSLYRCSRGIPRVINSLANMSLLEGFAKDAHVIDEGIVYSAAKEIGLNGYCKDKEKA
                     "
     gene            1986..2600
                     /locus_tag="VMU21_13040"
     CDS             1986..2600
                     /locus_tag="VMU21_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUO78491.1"
                     /translation="MDIAKIRKKLKESGQEDMAKPGAEPEATPHREEGHTLTAPPPSG
                     EASREKEAAETAQKEEKGEDFLVELLTFTLGNEEYAFRIDEIHEIIRPQRVTMIPKTG
                     SHLRGITSLRGKIIPVVDLRRLLSLNGNTVEEAKQKILILKGPKGPFGAIIDRVVGVI
                     RPSGSKIVETPPHLPEAQMRFIEGVAVVDGRFISIIRTEEAASI"
     gene            2621..5881
                     /gene="phnD"
                     /locus_tag="VMU21_13045"
     CDS             2621..5881
                     /gene="phnD"
                     /locus_tag="VMU21_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HUO78492.1"
                     /translation="MQNARFHKPHGIKNKIIWKFIVPTTVIVILVSCMSVFVVAGYVR
                     EQIRGRAHDHIQAMIGQVIESLNVTNTLELEKVRASMRVLMSEGLALGTPTLGESTSA
                     GPETVQNLFLGSRPQANNFVLVDHVKSLMGGTATLFVKKENDFVRVSTNVMKDDGSRA
                     VGTLLDPKGKAIAALREGKAFYGQVDLLGRPYITGYEPMYDKKNTLIGVWYVGYPVAT
                     FSHLGEIISSTKILDNGFIALVDDRGNVRFKSKDGSEDIVGKIVKQTSSAESGAWEIV
                     RKPFEPWGFQVVAAYPVTDITRRMTKAMGIVILCGLAVTCFLIVAFFFLVRKIVIAPL
                     RAIGAAATGISEGDLTFAVHVKSEDELGRLSDLLSESFRSLGRMLQRLRELSARISGV
                     AEEVEKESRKVVRSSEVETEAITNISHAVEELNATAMEIAESTEGLAASVEETSASVA
                     QMLSSINNVNDNIHELTTAVETTSSSIEELSATIKEVASNAAELAETSEESLSAVSEI
                     TSAIKEVEISAKESSRLSEKVTSDAATFGMTSVEKTMEGMKKIKASVEHTAEFIKRLG
                     GRSDEIGKILTVIDEVTDQTTLLALNAAILAAQAGEHGKGFSVVADEIKDLAERTTFS
                     TQEIASLIQSVQQEVKNAVDAMQQGLRSVDEGFSLSSEAATSLKKIVDSSKRSSQMTL
                     SIERATTEQSKAAKLVSEAVGRVRDMTDQIAKATAEESTGILHIMKAIEKMRDAALGV
                     NKATEEQSMSSRQISQSMEFVSERSRQIAKSLSEQKVGTKQILNAVEGVKEVPEENRA
                     LAFRISSTLENLHKDSEILKVEMERFRFTEEQGATLKLGIVPLESPAAMLKKFGPLSE
                     YLGKKTGKTAEIKVAVDFESTIRDIGEGVTQLCFMGAATYLWANKQYGVNVLVKALRK
                     GKPYTHSVIITRTDADIQSLDELKGRTFAFGDRQAATSYVVPRAMLKDAGVDINDLRY
                     YNFLGHNDAVASAVLKGDYDAGGVRESTAYAFQDQGIRFLKFSDEVPEFNICYHRSLD
                     ERSLSLIKTTLISLDVSTPEGAAILKSIDKDYTGFVEADDSDYDSIREKMSKLEIL"
     gene            5989..6471
                     /locus_tag="VMU21_13050"
     CDS             5989..6471
                     /locus_tag="VMU21_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUO78493.1"
                     /translation="MAKVAVVYAGQEEFGIDINKVVEILKAQRIHLIPQLPSFLSGMV
                     NIRGEVIPLVDLRKRFGIHPSSASERIIVVRTGQEKVGLLVDGVREIVNLEPHEISAP
                     PSVFKGLRTEYLIGIGKKGESIIILLNLDNLLTSEEKIQLQEASKTVEVSRAGDEKTS
                     "
     gene            6455..8425
                     /locus_tag="VMU21_13055"
     CDS             6455..8425
                     /locus_tag="VMU21_13055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUO78494.1"
                     /translation="MKRLLSDEEIEARRKAVLELKGKSPEESVPPLLKAMEDVSWRVR
                     KTASEILMDGYAPDYYIRGLIQLLYLEENAGARNSAIETLVRLGKKVTPLLIEGFHTL
                     NRDVRKFIIDVIGEVKDRRALPLLLKALKDEDDNVRASAVEHLGQMGDLSVVDALIEI
                     LESGDLWTAFPAADALGRIGDKKAVPSLLNALSVKALREPALKGLGHLSAPETLQHII
                     PFLMDPSKTIQEETVRTIGVFYHKGVSADFISGIMNKLCGPEIIAKLVSHAWSKKTDV
                     RVTAILLLGLMQDERALGPLLELSTEESLVEDVKRALTFIGKSKPESLLPLFQADSLY
                     QRRFITEVAVDVASPLYYPVFETLLEDEDGHMRALAAMGLSRIHDPRAVGPIEKLLSD
                     PYEDVQEAAVIALSDLGEGIETGEIIHYLKDKNPILRKNAALLLGRLEATESVNALGF
                     ALKDGDVSVRQAVVEALSSMKTEESVKYLVFALTDEKAQIRASACLSLGSMGSDRALE
                     PLILLLLTDSDDTVRVSAARSLGMIGERGAVNHLIDVLGDRNGFVVVTAMESLGKLGG
                     DEAKGALIRMLNSEEREIKRTAIRALSSFEGSEGIILPYLNDPDWATRTAAVKALSAR
                     ANERVKHEIEKSYEREEDPAVRRTIEEYFDVR"
     gene            8415..9287
                     /locus_tag="VMU21_13060"
     CDS             8415..9287
                     /locus_tag="VMU21_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HUO78495.1"
                     /translation="MFDREEAIPLREVEFRLLRDLIKEYCGLFFDDGSMFLLEKRLSR
                     RLKVHQLNDFRDYYRFLLYDRKRDEELTCVIDLLTVNETFFFREQNQLKAFSDEILPE
                     LRETNRESKRIRIWSAGCSTGEEPYTIAMLALEYGYLYGWDIEILGSDINQRVLTTAR
                     RGVYRKNSFRSTGRSFVDKYFAEENGSFTISEEVKRLVSFSCLNLLDPFKAKFVGKMD
                     VIFCRNVLIYFDQNARKKVVDTFYDSIVEGGYLLLGHAESLMNISTSFALRHLKNDMV
                     YQKPILQERTAHVR"
BASE COUNT         2513 a         2162 c         2717 g         2005 t
ORIGIN      
        1 agtacctatg tgagggttga tatcgagaag ctcgacagga tactgaacac cattagcgaa
       61 ctcaatctcg cgagatcggc gacaaacaga atcgctgcgg aaatgatgga caccttcggt
      121 cattctgccc ttgtgagcga tatcttcaag atctcccaga cctttgagcg taggattgcc
      181 gatatcaccg agcaggtgct ggaaataagg atgattccca taggacagat attctcaagg
      241 ctcggtcagg tcatacggcg gtactcgcgc gattcaggga agcagataga gctcgctctt
      301 tacggggaag acacagagat tgataagtac ctcgcggaag agatcattga ccccctgatg
      361 catatcgtga ggaatgccat tgatcacggc atagagacag cggaagagag aaaaagggca
      421 gggaaagcgg aagccggtag catcgtgctg aaagcatttc agaggggaaa ccatgtggtg
      481 atcgaggtcg gggatgacgg ttcggggatt gatcttgaac aggtcaggaa cactgcactg
      541 gggaaagggc tcatcacccc gggggctgaa ctcaacgacc gtgaaatact tgacttcata
      601 ttcatgccgg ggttcagtac caagtccact gtaagcgaaa tttcggggag aggcgtgggg
      661 atggatgtgg tgaaggaaaa ggtgtcgagc cttggtggtt ttgtggatgt ggagacagta
      721 aagggaaagg cgacaacctt tgtcctcacc gtgcctatca cccttgcgat actgaaggca
      781 ctcatcgtga ggataggtcc tgaacgcttt gccatccccc tcacgtcctt gtcagagagc
      841 ctgcttattg agcacaggag catccagacc atcgagggaa aggaagtata taaccttagg
      901 ggagagatgc tgcccatcgt gggcatcgca aagatgttcg gactggaagc cgacgttgct
      961 gagaagtcct atgcggtggt ggtgggctac ggagaacgga ggataggtct ttcggtggat
     1021 gagcttatcg ggcagcacga gattgtcatt aaatccttgg gcgattattt cagggggatg
     1081 cgtgggtttg cgggcgctgc ggagataggc aaacacgagg tcattttggt catcgatgtg
     1141 gagtccatca tagaagaggc tctctccaag cagaaggtga tacaccatgt atgaagtctt
     1201 ttacggattc aggacaagac ccttcagcaa gacgcctgat ccgcggtttc tctttatgtc
     1261 gagaacacac gaagaagctc tcgcaaggct ccagtatgca gtggaagaac gggagatcgc
     1321 ggtgctcacc ggcgagattg gctgcggaaa gacgaccctc acaagggcgc ttatggattc
     1381 ccttgatgac aagtacaggg tcatgttcat ccttaaccca aggctgacct cctttgattt
     1441 cgtaaagacc atcagtgagc gactccaatg cagcatctcc tctcctcaca agagtgacct
     1501 ccttgaagcc atctatgcaa agctctatga ggagtaccgg tccgggataa cccctgtgat
     1561 gattattgat gaggcacacc acatccctta ccgtgagacc ttcgaggaga tacggctgct
     1621 gacaaacttc cagatggatg atacaaacct cctgggtctt gttctcgtgg gacagccgga
     1681 cctgaggaga aagctggaac tgccctcgct tcgcgcattg aagcagagga ttggactctt
     1741 ctgccatctg gataccatcg gcgaagacga gataaggtcg tatattgacc acaggaccgc
     1801 ggttggcggc aggaataccc ctcttttcac tgagggcgca cttcgctccc tgtatcggtg
     1861 ttcccggggg atcccccggg tgattaacag cctcgcgaat atgtcccttc tggaaggctt
     1921 tgcgaaagac gcgcacgtaa tcgatgaagg tatagtatac tctgctgcaa aggaaatcgg
     1981 gcttaatgga tattgcaaag ataaggaaaa agcttaagga aagcggccag gaagacatgg
     2041 caaagccggg cgccgaacct gaggcaactc cgcataggga agaaggtcat acgcttaccg
     2101 cgccgcctcc ttcgggggaa gcttcgcgag aaaaagaggc agccgagaca gcccaaaaag
     2161 aggagaaggg tgaggatttt ctcgtcgagt tgctcacctt tacgctgggc aatgaagaat
     2221 atgccttcag gattgatgaa atacatgaga tcatacggcc ccagagggta accatgatac
     2281 caaagaccgg gtcccatctc cgggggatta cttcactaag gggaaagatc atccctgtgg
     2341 ttgacctgag aagattgctg tcccttaatg ggaacaccgt ggaagaagca aaacagaaaa
     2401 tacttatact gaaagggccg aaggggccgt ttggcgccat tattgacagg gtggtagggg
     2461 taatcaggcc gtcgggttcg aaaatcgtcg agaccccgcc gcacctgcca gaggcccaga
     2521 tgagatttat tgagggggtt gccgtcgtgg atggcaggtt catatccatc attcgcacag
     2581 aggaagctgc gagtatatga gagtgcgcag accataaaaa atgcagaatg cgagatttca
     2641 taagcctcat ggcataaaaa ataagataat ctggaagttc atcgtcccca caacagtaat
     2701 tgtcattctt gtgtcgtgta tgagcgtgtt cgtcgttgcg ggatacgtac gtgagcaaat
     2761 tcgcggacgc gcgcatgacc acatacaagc catgatagga caggtcatcg agagtcttaa
     2821 tgtgacgaac acccttgaac tcgagaaagt ccgtgcctcg atgcgggtgt tgatgagcga
     2881 gggacttgcg ctaggcacac ccacccttgg cgaatcaact tccgcgggac ccgagactgt
     2941 ccagaatctt tttctcggga gcaggccgca ggcgaataat tttgtgctgg ttgaccatgt
     3001 gaagtcatta atggggggga ccgcgacact ttttgtgaaa aaagaaaacg attttgtgcg
     3061 ggtgagcacg aatgtgatga aagatgacgg ttcccgtgcg gtcggcacgc tccttgaccc
     3121 caagggcaag gccatcgcag cgctacgcga aggtaaggca ttctatgggc aggttgacct
     3181 tctcggaaga ccgtatatca ccggatacga accgatgtac gacaagaaga atacactcat
     3241 aggagtctgg tacgtgggat atcctgtagc cacgttttcc catttgggag agataatctc
     3301 cagcacgaag atccttgata acggattcat tgcccttgtg gacgaccgcg ggaatgtgcg
     3361 cttcaagtca aaagacggtt ccgaagacat tgtggggaaa atcgtgaagc agacatcatc
     3421 ggctgaatcg ggggcatggg aaatagtgag aaagccgttt gaaccatggg ggtttcaggt
     3481 tgtcgctgca tatcccgtca ctgatatcac caggagaatg accaaggcaa tgggcatcgt
     3541 catactgtgt ggccttgcgg tcacctgttt cctgatagtc gccttctttt tccttgtgcg
     3601 aaagatcgtg atcgcaccgc tcagggccat aggggctgct gccacaggaa tttctgaagg
     3661 tgacctcacc tttgcagtgc atgtgaagag cgaagatgaa ctgggcaggt tgagcgatct
     3721 tctgagtgag tcattcagat cattgggaag aatgctccag agattaaggg agctttcggc
     3781 aaggatttcg ggggtggcgg aagaggtaga aaaagaatcg agaaaggtag taagaagctc
     3841 ggaagtggaa actgaggcta tcaccaacat atcccacgca gtggaggagc tcaatgcaac
     3901 cgctatggag attgcggaga gcacagaggg cctcgcggcc tcggtggagg agacctctgc
     3961 ttcggtagca cagatgctct cgagcatcaa taatgtgaac gacaatatcc atgaactcac
     4021 cactgcagtg gaaacgacct catcttccat agaagagctc tcggcaacca taaaggaggt
     4081 ggcgtcaaac gctgcggaac ttgccgagac atcagaggag agcctctccg cagtctcgga
     4141 aatcacgtcg gcaatcaaag aggtggaaat cagtgcaaaa gagtcctccc ggctttcaga
     4201 aaaggtcacc agtgacgcgg ccacctttgg catgacctct gtcgagaaga ccatggaggg
     4261 catgaagaag atcaaggcct ctgtggaaca taccgcagaa tttatcaaac ggctcggggg
     4321 aaggtcggat gagataggga agatacttac ggtaatcgac gaggtcacgg atcagaccac
     4381 actccttgcg ctcaatgcgg cgatattagc agcacaggca ggggaacacg ggaagggatt
     4441 ttcggtggtg gctgatgaga ttaaggacct cgcggaacgc actacgtttt ccacacaaga
     4501 aatcgcttcc cttatccagt ctgtccagca ggaggtgaag aacgcggtgg atgcaatgca
     4561 acagggactc aggtcagtgg atgagggatt cagcctttca agtgaagcgg cgacttcgct
     4621 taagaaaata gtggacagct caaagaggtc atcccagatg acgctgtcca tagaaagggc
     4681 cactacagag cagtcaaagg ctgcaaagct cgtctcagag gctgtgggac gggtgagaga
     4741 catgacggac cagatcgcaa aggccactgc tgaggaatcg accggaattc tccacatcat
     4801 gaaggcgata gagaaaatga gagatgcggc gctgggggtg aacaaggcaa cggaagagca
     4861 gagtatgagc agccgtcaga tttcccagtc catggaattt gtgtccgagc gtagccgtca
     4921 gatagcaaag tccctctcag agcaaaaagt gggaacaaaa cagatactca atgcggtgga
     4981 gggggttaag gaagttcccg aggagaaccg cgcactggca ttcaggatca gtagcaccct
     5041 cgagaacctt cacaaggatt ccgagatact caaggtcgag atggagcgct tcagatttac
     5101 ggaagagcag ggcgcgacac tgaaactagg cattgtccct cttgagtccc ctgcagccat
     5161 gctcaagaaa ttcggtcccc tcagcgagta tctcggcaag aaaacgggaa aaaccgcgga
     5221 aatcaaggtg gcggtggatt ttgaaagcac cattcgggat ataggagagg gtgttaccca
     5281 gctgtgtttt atgggagcag caacctacct ctgggccaat aagcaatatg gggtgaatgt
     5341 actggtaaag gcactcagga aggggaagcc gtacacgcat tctgtgatca tcaccaggac
     5401 tgacgcggac atacaatccc tcgacgaact taaaggaaga acctttgcat tcggtgatcg
     5461 ccaggcggca acaagctatg ttgtcccaag ggcaatgctt aaggatgcgg gcgttgacat
     5521 caatgacctc cgctattata acttcctcgg acataatgac gccgtcgcaa gtgcggtatt
     5581 gaagggtgat tacgacgccg ggggcgtgag ggagtccacc gcatacgcct tccaggatca
     5641 agggattaga ttcctcaagt tctcagacga ggtccctgag tttaatatat gttatcaccg
     5701 ttccctggat gaaaggtctc tctctttgat aaaaactacc ctcatttccc tggacgtctc
     5761 cacacctgaa ggcgcggcga tcctgaaatc cattgataaa gactatacag gttttgttga
     5821 ggccgatgac agcgactatg acagtatcag agaaaaaatg tcaaaactgg agatccttta
     5881 gaggaaggga gaaggtgagc gactcaagag gagcctgaag gcacacgtaa ggggctcaag
     5941 agcgtcatct gtgattggga gatcggggcg aacagggatg gtgaagagat ggcaaaggta
     6001 gccgtagtat atgcaggaca agaggagttt ggaatcgaca tcaataaggt ggtggagatt
     6061 ctcaaggcac aacggatcca tctcatcccc cagctcccct cttttctttc ggggatggtc
     6121 aatatcaggg gagaggtgat cccccttgtc gatctaagga agcgttttgg tatacacccc
     6181 tcatcagcaa gtgagcgcat cattgtggtg agaacaggcc aggaaaaggt ggggctcctt
     6241 gtggacggcg taagggagat cgtcaatctc gagccccatg agatcagcgc gcctccttcc
     6301 gtgtttaagg ggctgaggac cgaatatctt atcggtatcg ggaaaaaagg tgaaagcatc
     6361 atcattcttc tgaatctcga taacctgttg acttcagagg aaaagataca gcttcaagaa
     6421 gcgtcaaaga cagttgaggt gagccgtgca ggagatgaaa agacttctta gtgacgaaga
     6481 gatagaggct cggagaaaag cggtcctgga actcaaaggg aaatcgccgg aagaatcagt
     6541 tccccccctc cttaaggcca tggaagacgt aagctggagg gtgaggaaga ccgcgtccga
     6601 gatccttatg gacgggtacg caccggatta ttatatacgc ggccttatcc agctcctcta
     6661 cctggaggaa aacgcagggg caaggaattc cgccatagag acgcttgtac gactggggaa
     6721 aaaagtcacc cccttgttga tcgagggttt tcataccctg aacagggacg tgaggaaatt
     6781 catcattgac gtcataggag aggtgaaaga cagaagggca ttgcccttgc tgttaaaggc
     6841 gttgaaggat gaggatgaca atgtgagggc atccgcagtg gagcaccttg gccaaatggg
     6901 ggatctgtcg gtagtggacg ctctcataga aatactggaa agcggcgacc tatggacagc
     6961 ctttcccgct gctgacgccc ttggaaggat aggggataag aaagctgtac cctccctcct
     7021 taatgccctt tcggtgaagg cattgaggga gccggcgctc aaggggctcg gccatttgtc
     7081 agctcccgag accctgcagc acatcatccc ctttctcatg gacccttcca agacgatcca
     7141 ggaagagact gtgagaacca tcggggtctt ctaccataaa ggcgtctcag ctgattttat
     7201 ttcagggata atgaataagc tttgcggacc tgagattatc gccaagctgg tgtcccatgc
     7261 atggtctaag aaaaccgatg tgagggtgac cgcaatactc ctgcttggac tcatgcagga
     7321 tgagcgtgca cttggccccc tccttgaact ctccactgaa gagagtctgg ttgaagatgt
     7381 aaaacgcgcc ctcacattca tagggaaaag caagcctgaa tcccttctcc ctctcttcca
     7441 ggcggacagc ctgtatcaga ggagattcat cactgaggtt gcggtggatg tggcatcccc
     7501 tctgtattat cctgtttttg aaaccctcct tgaagacgag gacggccata tgagggcact
     7561 cgcagccatg ggtctttcga gaattcatga tccgagggcc gttggtccca tcgagaagct
     7621 gctctctgac ccgtatgagg atgtccagga ggcagccgtg atcgcactga gcgacctcgg
     7681 ggaaggcatc gagacggggg aaatcatcca ttacctgaaa gataagaacc ccatactcag
     7741 aaaaaatgcc gccctgcttc tcgggagact ggaggcaacg gagagcgtga atgccctggg
     7801 ttttgccctt aaggatgggg atgtttctgt cagacaggcg gtggttgagg ccctttcttc
     7861 catgaagacg gaagaatccg ttaaatacct cgtgttcgcc ctcactgatg aaaaagccca
     7921 gataagggct tccgcctgcc tgagtcttgg ctccatggga agcgacaggg cgctggagcc
     7981 tctcatcctc ctccttctca ctgattccga tgacacggta agggtttccg cagcccggtc
     8041 tctggggatg ataggggaga gaggggcagt gaaccatctt atcgatgtgc tcggtgaccg
     8101 taatgggttt gtggtagtaa cggccatgga atctttgggc aagctgggag gcgatgaggc
     8161 caagggtgcg ctcatcagga tgctcaactc tgaggaaaga gaaataaaaa gaacggccat
     8221 acgagccctt tcctcatttg aggggagtga ggggatcatc ctcccgtacc tcaatgatcc
     8281 ggactgggcc acaaggactg cagcggtaaa ggccctcagt gcgcgggcaa atgaacgggt
     8341 gaaacatgag atcgagaagt cctacgaaag ggaagaagac cctgcggtga gaaggacgat
     8401 tgaggagtat ttcgatgttc gatagggaag aagccatacc cctcagagag gttgagttca
     8461 ggcttcttag agatctgata aaagagtact gcgggctttt ctttgacgac ggatcaatgt
     8521 ttctcctgga gaaaagactc tcgaggagac tgaaagtcca tcagctgaat gacttcaggg
     8581 attactaccg cttcctcctt tacgacagga aaagggacga ggagctcacg tgtgtcatag
     8641 acctcctcac cgtgaacgag acatttttct ttcgggaaca gaaccagttg aaggcgttca
     8701 gcgatgagat actgccggag ctcagggaaa cgaacaggga gtcaaagaga atacggatat
     8761 ggtccgcagg gtgttccacc ggagaagagc cctataccat cgcgatgctc gccctggaat
     8821 acggatatct ctatggatgg gatatcgaga tactcgggag cgatatcaat caaagggtat
     8881 tgaccacggc gaggcgcggg gtatacagga aaaactcctt caggtcaacg gggaggtctt
     8941 tcgttgacaa atatttcgca gaagagaacg gttctttcac catatcggag gaggtaaaac
     9001 gccttgtgag tttcagctgc ctgaacctcc tggacccctt caaagcgaaa tttgtgggca
     9061 agatggatgt aatcttctgc aggaatgttc tgatctactt tgaccagaat gcgaggaaaa
     9121 aagtggtgga caccttttat gacagcattg tggaaggcgg ctatctgctc cttggtcacg
     9181 cagaatctct catgaacatt tccacctctt ttgccctcag gcacctcaag aacgatatgg
     9241 tttaccagaa accgatactc caggagcgta ccgcgcatgt ccgataaaat aggcgtcctt
     9301 attgtggatg attctgcgta cagcaggcag accatcaagg gaatgctcga gacggataag
     9361 ggaatcgagg tggtgggaat cgcatcagac gggatcg
//
LOCUS       DATKOU010000273         4324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Thermodesulfovibrionales bacterium isolate SMAG_U14772
            k141_4617142, whole genome shotgun sequence.
ACCESSION   DATKOU010000273 DATKOU010000000
VERSION     DATKOU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938277
            Sequence Read Archive: SRR8879131
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfovibrionales bacterium (soil metagenome)
  ORGANISM  Thermodesulfovibrionales bacterium
            Bacteria; Nitrospirota; Thermodesulfovibrionia;
            Thermodesulfovibrionales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 01:52:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,569
            CDSs (with protein)               :: 2,569
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Thermodesulfovibrionales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4617142"
                     /isolate="SMAG_U14772"
                     /isolation_source="soil"
                     /db_xref="taxon:2814272"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.5008 N 80.2398 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VMU21_13065"
     CDS             complement(<1..618)
                     /locus_tag="VMU21_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUO78496.1"
                     /translation="MTPFITTYLGYLIYQREKLLKKVVTEKAKEDAILGAIGEGISIQ
                     DTHFRILYQNQLHKEMVGDHIGECCYKAFQNREEVCEGCHLAMTFNDGKTHKKEQQIV
                     TDKGTVYLEITASPLTDLSGKIISGIDVVRDVTERRLMEEKLRQSEKKYRELVETASS
                     IILTWDTAGNVTFLNDFAERFFGFSKEELLGRNVVGTIVPPAESSG"
     gene            complement(1026..1469)
                     /gene="rimI"
                     /locus_tag="VMU21_13070"
     CDS             complement(1026..1469)
                     /gene="rimI"
                     /locus_tag="VMU21_13070"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HUO78497.1"
                     /translation="MKQVFIRDMQEEDIQSVLKIEKMSFSFPWAERSFFNEVRKPRSL
                     TKVAVVDNEIAGYLCAEYVLDEGHVLDLAVHPDYRRLGIATALVEQILEELKKRACRF
                     LYLEVRTSNYLAKRLYQGFGFRIVGTRKKYYVSPTEDAVIMMLEV"
     gene            complement(1580..2287)
                     /gene="tsaB"
                     /locus_tag="VMU21_13075"
     CDS             complement(1580..2287)
                     /gene="tsaB"
                     /locus_tag="VMU21_13075"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HUO78498.1"
                     /translation="MKILAIETSTMLGGIAIIGESEGLIFESRLNVRTAHSERLMTEI
                     DHALKQGGMNISEIDVFGIAIGPGSFTGLRIGLSTVKGFSYSTGKPVVSVPTLEAFAW
                     NFPYSPYPVCTMFDARKKEVYAAVFRWRDGGFERMVNETSVRVSEMVKSLEGQVLFAG
                     EGAILYRQVITDILGDLAIFPPLHHMVPSPSNVAHLGLKKALRGEFSEPAGLVPFYLR
                     KSEAELKIDSRIGTRNP"
     gene            complement(2319..3605)
                     /locus_tag="VMU21_13080"
     CDS             complement(2319..3605)
                     /locus_tag="VMU21_13080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUO78499.1"
                     /translation="MNNPQEHIRQERFSALYVRDFRIFWIGQLISFSGTWMQSTAQGW
                     LVYSLTKSPLYLGIVATAASLPVLLFTLIGGVVADRLNKRTLLIITQTFSMVPALSLA
                     ILINLEVVTVWQIVFFAALLGTVNAFDVPSRQSFLVDMVQKGRLMNAIALNSAAFNGA
                     RIMGPVIAGITIAAIGVAACFYVNAVSFLAAIVALLMIKTRSNGDGEKSMKSMGEDRR
                     LHLSVVLDDLSEGLRYVKSEKDVFRIMLLVALFSLFGIPFVTLLPVFAADILMVGPKG
                     LGILAGSSGVGSLAAALFIAFRGEIEKKGRFMSGAGVLFGVSLLFFSLSGDYHVSMVI
                     LVLAGLGMVSFLAVANTFIQLTVPDVLRGRVMSVYALVFLGIAPMGNAVMGAIANEVG
                     TDKAVSIGSSLCLFAVLLSWKHLKTMDKTAQVTQGP"
     gene            complement(3609..4052)
                     /gene="dtd"
                     /locus_tag="VMU21_13085"
     CDS             complement(3609..4052)
                     /gene="dtd"
                     /locus_tag="VMU21_13085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HUO78500.1"
                     /translation="MKALIQRVSEASVKVNGDTVGSIGRGILLFVGIEKGDGEKDIHY
                     LINKIANLRIFGDAEGKMNLSVRDIKGSILAVSQFTLSADCKKGNRPSFDNAETLQRA
                     EQLYSLFVRTLSDSGIPVSKGNFGAHMEVLLVNDGPVTFLLDSRR"
BASE COUNT         1121 a         1124 c          990 g         1089 t
ORIGIN      
        1 gccggacgat tcggccgggg gaacgattgt gcccaccaca tttctgccga gcagctcttc
       61 ttttgagaat ccgaagaacc gttctgcaaa atcgttcagg aaggtgacgt ttcccgccgt
      121 gtcccaagtc aggatgatcg aactcgctgt ctctaccagt tcacggtact tcttttcact
      181 ttgccgcagc ttttcctcca tcagcctgcg ctcggtgacg tccctgacca cgtcaatgcc
      241 agagataatt ttccccgata aatcggttaa cggagatgct gtaatttcaa ggtatactgt
      301 tcctttgtcg gtaactatct gctgttcctt tttgtgagtc tttccgtcat tgaaggtcat
      361 tgcaagatga catccttcac agacctcttc cctgttctga aaggccttat aacagcattc
      421 ccctatgtgg tctcccacca tctccttatg gagttgattt tgatagagaa tcctgaaatg
      481 tgtatcttga atgctgatcc cttctcctat tgccccaaga attgcgtctt ccttggcctt
      541 ctctgttact acctttttga gaagtttttc tctctgataa atgagatacc ctaaataggt
      601 agttataaat ggcgtcaaga aaataacgac atggaaaata gtttgttccg aatatttcgc
      661 cgaaagtaaa tgcgttaaca gggcggatcc gtattcccgg tattcataaa aaagatagtt
      721 gcccagcaag aaccaaccta tcagtgcaat tataattttt aacatctcac caacccattt
      781 ccgaaaattc tgttcaagtc cttcttatta tgcaatttta gcataaaatt actcgcttaa
      841 gattgcttga tttaccctca accagagttt cctcttgcct gatcataggg tggcaagtca
      901 ccgtgcgcat ttctgtcctg acttatcata ttctgaagtt tacagaactg atgagtatcc
      961 gccgacgggc tcacaaaaaa gggcacggct cattacatga cgcttcatca caaagggcaa
     1021 aaatactaca cctccagcat catgatcacc gcatcttcgg tgggtgagac atagtacttt
     1081 tttctggtac ccacaattct gaagccgaag ccctggtaga gccttttcgc gagatagttt
     1141 gacgtcctga cctcaaggta taagaacctg cacgccctct tcttaagttc ttcaaggatt
     1201 tgttccacaa gggctgtggc gatccccaac ctccggtaat cgggatggac tgcaaggtca
     1261 aggacatggc cttcgtccag cacatattca gcgcacagat atccggcaat ttcattatcc
     1321 accaccgcaa cctttgtgag agaacggggc ttacgcacct cattaaagaa ggacctctct
     1381 gcccagggaa atgaaaatga cattttctct atcttgagaa cggactggat gtcctcctcc
     1441 tgcatgtccc ttatgaagac ctgtttcatt ttttttgtta agggtaatcc cacgaagtca
     1501 gatgtagcca ttccagaaga agaagccggg gagagaggcc acaacgagat gcacacctgc
     1561 acctcttcgg acatatttat tatggattcc tggtccctat tcgcgaatct atcttcagtt
     1621 ctgcctctga ctttctcagg taaaagggga cgaggcccgc tggctcagaa aattctcccc
     1681 ttaacgcctt cttcaggcca aggtgggcaa cgtttgacgg agaggggacc atatggtgca
     1741 ggggagggaa tatggcaagg tctcccaaga tatccgtaat gacctgccgg taaaggatgg
     1801 ctccttcgcc tgcaaaaagc acctgccctt caagggactt taccatctcg gataccctta
     1861 ccgatgtctc atttaccatc ctctcaaaac cgccatctct ccacctgaat accgctgcat
     1921 agacctcctt cttccttgca tcgaacattg tacagacagg ataagggctg taagggaagt
     1981 tccatgcaaa ggcctccagg gtcgggacag agacgacggg cttacccgtc gaataggaga
     2041 accccttcac agtactcaga ccgatcctca gccctgtaaa tgaaccgggg cctatggcaa
     2101 ttccaaacac atcgatctct gaaatgttca tgcctccctg tttcagcgca tggtctattt
     2161 ctgtcatcag cctttccgag tgggcggttc tcacattgag cctcgactca aagataaggc
     2221 cctctgattc gccgattatg gcgatacctc ccagcatggt ggatgtttcg atggcgagaa
     2281 tcttcattcc gaaagcctaa tacatcatgg actgtttttc aagggccttg cgtaacttga
     2341 gcggttttat ccattgtttt gaggtgcttc caggagagga gtaccgcaaa aagacagagg
     2401 gacgagccta tggagacggc cttgtctgtt cccacctcat tcgcgatggc tcccataacc
     2461 gcatttccca tgggcgcaat accgaggaaa accagggcat atacactcat cacccttccc
     2521 ctcaggacat cagggacagt gagctgaata aacgtatttg ccacggcaag aaaactgacc
     2581 atcccgagtc cggcaaggac gaggataacc atcgaaacat ggtaatctcc ggagagggag
     2641 aaaaagagaa gcgaaacacc gaagagcact cctgccccgg acatgaacct tcccttcttc
     2701 tctatctcgc ctctgaacgc gataaagagc gcggccgcaa gggaacccac tcccgaggaa
     2761 ccggccagaa tgcccagccc cttgggccca accataagaa tgtccgctgc aaacacggga
     2821 agaagggtga caaagggtat accaaagagg ctgaacaagg caacaaggag cataatcctg
     2881 aagacatcct tttcgctttt cacataccgg agtccctcgg agaggtcgtc caaaacaaca
     2941 gataggtgaa ggcggcgatc ctcacccatg gacttcatgg atttttcccc gtcaccattg
     3001 ctcctggttt ttatcataag gagtgcaacg atggctgcga gaaaactcac tgcattcacg
     3061 taaaagcatg ccgccacccc aatggctgct atggtgattc cggcaatgac aggacccatg
     3121 atacgcgccc cgttgaaggc tgcggagttc agtgcaatgg cattcatgag acggcccttc
     3181 tggaccatat ccacaagaaa agactgcctc gaaggtacat caaaggcatt cactgtgccc
     3241 agcagtgctg caaaaaaaac gatctgccag acggtgacca cctccagatt gatgagaatc
     3301 gcaagggaaa gggcgggtac catagagaag gtctgggtga tgatgagaag cgtcctcttg
     3361 ttcagtctgt ccgcaaccac gcctccgatg agggtgaaga ggagaacggg cagagatgct
     3421 gcggtggcaa caataccgag atataaaggg gacttggtga gggaatacac aagccatccc
     3481 tgggcagtcg actgcatcca tgtcccggag aaggagatga gctgtcctat ccagaagatg
     3541 cggaaatccc tcacatagag ggcagaaaat ctttcctggc gtatatgttc ctgagggtta
     3601 ttcatgcatc acctgcgtga atcaaggaga aatgttacgg gaccgtcgtt caccagaagc
     3661 acctccatat gtgcgccgaa gtttcctttg gagaccggaa tcccggagtc tgaaagcgtc
     3721 cggacaaaaa ggctatagag ttgttccgca cgctggagtg tctccgcatt gtcaaaggaa
     3781 gggcgatttc cctttttgca gtcagcggaa agggtgaact gggaaactgc caggatgctc
     3841 ccctttatgt ccctcacgga aaggttcatc ttgccctcag catcaccaaa aatcctgagg
     3901 tttgcaatct tattgataag gtagtggatg tccttctccc cgtccccctt ttcaatcccc
     3961 acaaagagga gaattcccct gccgatactt cccaccgtat cgccgtttac cttcaccgat
     4021 gcctctgaaa cccgttgaat cagcgctttc atttcggttc tatgcctgtt ccttaaccgt
     4081 gatatcaaaa actgtcttgt cattttatca cacaatgtgt actaattaag gagtgttttc
     4141 cagttgcgag aggtttgttg tcgtcaaagg atatttcttt tgacgacaac gggcaaaggc
     4201 atactatttt cttcctggag tcaacactgt aattttcttg ccacagcata aggtttctct
     4261 tggcattaga tggacagaga gaaacttacc tgtcaaggtt acttgcagta attaataatg
     4321 ccgg
//
